BLASTP 2.2.25+
Reference:
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database
search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
15,229,318 sequences; 5,219,829,388 total letters
Query= Rv0064A
Length=79
Score E
Sequences producing significant alignments: (Bits) Value
gi|307082526|ref|ZP_07491639.1| antitoxin [Mycobacterium tubercu... 154 3e-36
gi|167970592|ref|ZP_02552869.1| hypothetical protein MtubH3_2217... 154 6e-36
gi|121635975|ref|YP_976198.1| antitoxin [Mycobacterium bovis BCG... 152 2e-35
gi|336118170|ref|YP_004572939.1| hypothetical protein MLP_25220 ... 46.6 0.001
gi|315446138|ref|YP_004079017.1| hypothetical protein Mspyr1_463... 43.1 0.014
gi|152966079|ref|YP_001361863.1| hypothetical protein Krad_2114 ... 42.0 0.031
gi|269957932|ref|YP_003327721.1| hypothetical protein Xcel_3162 ... 39.7 0.14
gi|134102275|ref|YP_001107936.1| hypothetical protein SACE_5827 ... 39.7 0.17
gi|333025633|ref|ZP_08453697.1| hypothetical protein STTU_3137 [... 39.3 0.17
gi|87124165|ref|ZP_01080015.1| hypothetical protein RS9917_11156... 37.7 0.54
gi|86740206|ref|YP_480606.1| hypothetical protein Francci3_1501 ... 36.2 1.6
gi|87300650|ref|ZP_01083492.1| hypothetical protein WH5701_04360... 35.8 2.1
gi|330465117|ref|YP_004402860.1| hypothetical protein VAB18032_0... 35.4 2.9
gi|333989357|ref|YP_004521971.1| hypothetical protein JDM601_071... 35.0 3.5
gi|337766708|emb|CCB75419.1| conserved protein of unknown functi... 34.7 4.8
gi|54026211|ref|YP_120453.1| hypothetical protein nfa42400 [Noca... 34.7 5.1
gi|325570811|ref|ZP_08146494.1| hypothetical protein HMPREF9087_... 34.3 5.6
gi|154246135|ref|YP_001417093.1| hypothetical protein Xaut_2192 ... 34.3 5.9
>gi|307082526|ref|ZP_07491639.1| antitoxin [Mycobacterium tuberculosis SUMu012]
gi|308379686|ref|ZP_07487139.2| antitoxin [Mycobacterium tuberculosis SUMu010]
gi|308380887|ref|ZP_07491354.2| antitoxin [Mycobacterium tuberculosis SUMu011]
gi|308356182|gb|EFP45033.1| antitoxin [Mycobacterium tuberculosis SUMu010]
gi|308360249|gb|EFP49100.1| antitoxin [Mycobacterium tuberculosis SUMu011]
gi|308367720|gb|EFP56571.1| antitoxin [Mycobacterium tuberculosis SUMu012]
Length=83
Score = 154 bits (390), Expect = 3e-36, Method: Compositional matrix adjust.
Identities = 79/79 (100%), Positives = 79/79 (100%), Gaps = 0/79 (0%)
Query 1 MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEAIEILEQALASTA 60
MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEAIEILEQALASTA
Sbjct 5 MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEAIEILEQALASTA 64
Query 61 SPGISRETIEASRRELRGG 79
SPGISRETIEASRRELRGG
Sbjct 65 SPGISRETIEASRRELRGG 83
>gi|167970592|ref|ZP_02552869.1| hypothetical protein MtubH3_22173 [Mycobacterium tuberculosis
H37Ra]
gi|253796981|ref|YP_003029982.1| antitoxin [Mycobacterium tuberculosis KZN 1435]
gi|254548996|ref|ZP_05139443.1| antitoxin [Mycobacterium tuberculosis '98-R604 INH-RIF-EM']
39 more sequence titles
Length=79
Score = 154 bits (388), Expect = 6e-36, Method: Compositional matrix adjust.
Identities = 79/79 (100%), Positives = 79/79 (100%), Gaps = 0/79 (0%)
Query 1 MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEAIEILEQALASTA 60
MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEAIEILEQALASTA
Sbjct 1 MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEAIEILEQALASTA 60
Query 61 SPGISRETIEASRRELRGG 79
SPGISRETIEASRRELRGG
Sbjct 61 SPGISRETIEASRRELRGG 79
>gi|121635975|ref|YP_976198.1| antitoxin [Mycobacterium bovis BCG str. Pasteur 1173P2]
gi|224988448|ref|YP_002643135.1| hypothetical protein, antitoxin [Mycobacterium bovis BCG str.
Tokyo 172]
gi|121491622|emb|CAL70080.1| Conserved hypothetical protein, antitoxin [Mycobacterium bovis
BCG str. Pasteur 1173P2]
gi|224771561|dbj|BAH24367.1| hypothetical protein, antitoxin [Mycobacterium bovis BCG str.
Tokyo 172]
gi|341599991|emb|CCC62659.1| conserved hypothetical protein, antitoxin [Mycobacterium bovis
BCG str. Moreau RDJ]
Length=79
Score = 152 bits (384), Expect = 2e-35, Method: Compositional matrix adjust.
Identities = 78/79 (99%), Positives = 78/79 (99%), Gaps = 0/79 (0%)
Query 1 MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEAIEILEQALASTA 60
MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAE IEILEQALASTA
Sbjct 1 MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEVIEILEQALASTA 60
Query 61 SPGISRETIEASRRELRGG 79
SPGISRETIEASRRELRGG
Sbjct 61 SPGISRETIEASRRELRGG 79
>gi|336118170|ref|YP_004572939.1| hypothetical protein MLP_25220 [Microlunatus phosphovorus NM-1]
gi|334685951|dbj|BAK35536.1| hypothetical protein MLP_25220 [Microlunatus phosphovorus NM-1]
Length=76
Score = 46.6 bits (109), Expect = 0.001, Method: Compositional matrix adjust.
Identities = 22/49 (45%), Positives = 31/49 (64%), Gaps = 0/49 (0%)
Query 1 MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEAI 49
M ++Q++D+PE+ RRRA A QSLQ Y+ TKL+E R AE +
Sbjct 1 MPSVQIKDVPEETHRVLRRRAAEAHQSLQEYLLTKLVEDARVPTMAEVL 49
>gi|315446138|ref|YP_004079017.1| hypothetical protein Mspyr1_46320 [Mycobacterium sp. Spyr1]
gi|315264441|gb|ADU01183.1| hypothetical protein Mspyr1_46320 [Mycobacterium sp. Spyr1]
Length=76
Score = 43.1 bits (100), Expect = 0.014, Method: Compositional matrix adjust.
Identities = 17/37 (46%), Positives = 26/37 (71%), Gaps = 0/37 (0%)
Query 1 MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLI 37
M ++Q++D+P+D R RA A QSLQ Y+R++LI
Sbjct 1 MPSVQIKDVPDDTHRVLRERAARAHQSLQEYLRSRLI 37
>gi|152966079|ref|YP_001361863.1| hypothetical protein Krad_2114 [Kineococcus radiotolerans SRS30216]
gi|151360596|gb|ABS03599.1| conserved hypothetical protein [Kineococcus radiotolerans SRS30216]
Length=76
Score = 42.0 bits (97), Expect = 0.031, Method: Compositional matrix adjust.
Identities = 17/38 (45%), Positives = 26/38 (69%), Gaps = 0/38 (0%)
Query 1 MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIE 38
M ++Q++D+P++ RRRA A QSLQ Y+ KL+E
Sbjct 1 MPSVQIKDVPDETHTVLRRRAALAHQSLQEYLLAKLVE 38
>gi|269957932|ref|YP_003327721.1| hypothetical protein Xcel_3162 [Xylanimonas cellulosilytica DSM
15894]
gi|269306613|gb|ACZ32163.1| conserved hypothetical protein [Xylanimonas cellulosilytica DSM
15894]
Length=77
Score = 39.7 bits (91), Expect = 0.14, Method: Compositional matrix adjust.
Identities = 17/36 (48%), Positives = 26/36 (73%), Gaps = 0/36 (0%)
Query 1 MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKL 36
M+T+QVR++P+DV ++RA AGQSL Y+ +L
Sbjct 1 MSTLQVRNVPDDVGRVLKQRAARAGQSLSEYVLAQL 36
>gi|134102275|ref|YP_001107936.1| hypothetical protein SACE_5827 [Saccharopolyspora erythraea NRRL
2338]
gi|291003762|ref|ZP_06561735.1| hypothetical protein SeryN2_04502 [Saccharopolyspora erythraea
NRRL 2338]
gi|133914898|emb|CAM05011.1| hypothetical protein SACE_5827 [Saccharopolyspora erythraea NRRL
2338]
Length=76
Score = 39.7 bits (91), Expect = 0.17, Method: Compositional matrix adjust.
Identities = 21/51 (42%), Positives = 29/51 (57%), Gaps = 0/51 (0%)
Query 4 IQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEAIEILEQ 54
+Q+RD+PEDV T + RA AGQS ++R L + AE + LEQ
Sbjct 5 VQIRDVPEDVYRTLKIRAVEAGQSYSEFLRGLLTQAASRPPMAEMVRRLEQ 55
>gi|333025633|ref|ZP_08453697.1| hypothetical protein STTU_3137 [Streptomyces sp. Tu6071]
gi|332745485|gb|EGJ75926.1| hypothetical protein STTU_3137 [Streptomyces sp. Tu6071]
Length=75
Score = 39.3 bits (90), Expect = 0.17, Method: Compositional matrix adjust.
Identities = 26/75 (35%), Positives = 44/75 (59%), Gaps = 4/75 (5%)
Query 1 MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEAIEILEQALASTA 60
MAT+Q+R+L ++ RRRA +G+SLQ Y+R ++ E R + E+L+ L+ A
Sbjct 1 MATVQIRNLDDEAYAILRRRAAESGRSLQEYLRIEVEELAR---RPSMDEVLDD-LSDHA 56
Query 61 SPGISRETIEASRRE 75
++ + I A +RE
Sbjct 57 VSDVTMDEIVAIQRE 71
>gi|87124165|ref|ZP_01080015.1| hypothetical protein RS9917_11156 [Synechococcus sp. RS9917]
gi|86168734|gb|EAQ69991.1| hypothetical protein RS9917_11156 [Synechococcus sp. RS9917]
Length=74
Score = 37.7 bits (86), Expect = 0.54, Method: Compositional matrix adjust.
Identities = 21/53 (40%), Positives = 30/53 (57%), Gaps = 0/53 (0%)
Query 3 TIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEAIEILEQA 55
T+ +R +P+DVAE R A+ AG L T+ +L E R D AE ++ L A
Sbjct 4 TLYIRHVPDDVAERLERLASRAGLPLSTFALQELSETARRADNAELLQALPSA 56
>gi|86740206|ref|YP_480606.1| hypothetical protein Francci3_1501 [Frankia sp. CcI3]
gi|86567068|gb|ABD10877.1| hypothetical protein Francci3_1501 [Frankia sp. CcI3]
Length=78
Score = 36.2 bits (82), Expect = 1.6, Method: Compositional matrix adjust.
Identities = 19/39 (49%), Positives = 27/39 (70%), Gaps = 1/39 (2%)
Query 1 MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEG 39
MATI VR++PE+ + RA AG+SLQ Y+ +L+EG
Sbjct 1 MATIHVREVPEETLTILKVRAARAGRSLQQYV-LQLLEG 38
>gi|87300650|ref|ZP_01083492.1| hypothetical protein WH5701_04360 [Synechococcus sp. WH 5701]
gi|87284521|gb|EAQ76473.1| hypothetical protein WH5701_04360 [Synechococcus sp. WH 5701]
Length=74
Score = 35.8 bits (81), Expect = 2.1, Method: Compositional matrix adjust.
Identities = 20/53 (38%), Positives = 28/53 (53%), Gaps = 0/53 (0%)
Query 3 TIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEAIEILEQA 55
T+ +R +P+DVAE R A AG L T+ +L E R D A+ + L A
Sbjct 4 TLYIRHVPDDVAERLERLANRAGLPLSTFALRELSETARRADNADLLNALPSA 56
>gi|330465117|ref|YP_004402860.1| hypothetical protein VAB18032_05670 [Verrucosispora maris AB-18-032]
gi|328808088|gb|AEB42260.1| hypothetical protein VAB18032_05670 [Verrucosispora maris AB-18-032]
Length=84
Score = 35.4 bits (80), Expect = 2.9, Method: Compositional matrix adjust.
Identities = 17/38 (45%), Positives = 26/38 (69%), Gaps = 1/38 (2%)
Query 1 MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIE 38
M +Q+RD+PE+V + +A A GQSLQ Y+ +L+E
Sbjct 1 MVALQIRDVPEEVRDALAAQAKARGQSLQAYL-LELVE 37
>gi|333989357|ref|YP_004521971.1| hypothetical protein JDM601_0717 [Mycobacterium sp. JDM601]
gi|333485325|gb|AEF34717.1| conserved hypothetical protein [Mycobacterium sp. JDM601]
Length=76
Score = 35.0 bits (79), Expect = 3.5, Method: Compositional matrix adjust.
Identities = 20/49 (41%), Positives = 29/49 (60%), Gaps = 3/49 (6%)
Query 4 IQVRDLPEDVAETYRRRATAAGQSLQTYMRTKL---IEGVRGRDKAEAI 49
IQ++D+PE+V + RA A GQS Q Y+R+ L + R R EA+
Sbjct 5 IQIKDVPEEVRDALAARAEARGQSTQAYLRSLLEREYQAQRNRQLLEAL 53
>gi|337766708|emb|CCB75419.1| conserved protein of unknown function [Streptomyces cattleya
NRRL 8057]
Length=76
Score = 34.7 bits (78), Expect = 4.8, Method: Compositional matrix adjust.
Identities = 24/79 (31%), Positives = 38/79 (49%), Gaps = 4/79 (5%)
Query 1 MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEAIEILEQALASTA 60
M + +RD+P+D +T + RA AG+SLQ Y + AE + L +
Sbjct 1 MTALTIRDVPDDQIQTLKVRAAQAGKSLQAYFLDLIARETSKPTMAEMVARLNRE----T 56
Query 61 SPGISRETIEASRRELRGG 79
+ G+S E + A+ E R G
Sbjct 57 TAGVSTEDVLAAIDEARTG 75
>gi|54026211|ref|YP_120453.1| hypothetical protein nfa42400 [Nocardia farcinica IFM 10152]
gi|54017719|dbj|BAD59089.1| hypothetical protein [Nocardia farcinica IFM 10152]
Length=154
Score = 34.7 bits (78), Expect = 5.1, Method: Compositional matrix adjust.
Identities = 24/74 (33%), Positives = 33/74 (45%), Gaps = 0/74 (0%)
Query 5 QVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEAIEILEQALASTASPGI 64
Q DLP + + RRA A+G L Y+R +LI R +A+ + A I
Sbjct 80 QFYDLPAETLAVFARRAAASGTPLGEYVRRELIASARRTTVEDALAEFAEVSAGAPELNI 139
Query 65 SRETIEASRRELRG 78
E I A+ R RG
Sbjct 140 DMEAIAAAVRYARG 153
>gi|325570811|ref|ZP_08146494.1| hypothetical protein HMPREF9087_2783 [Enterococcus casseliflavus
ATCC 12755]
gi|325156321|gb|EGC68503.1| hypothetical protein HMPREF9087_2783 [Enterococcus casseliflavus
ATCC 12755]
Length=390
Score = 34.3 bits (77), Expect = 5.6, Method: Composition-based stats.
Identities = 22/78 (29%), Positives = 39/78 (50%), Gaps = 6/78 (7%)
Query 1 MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEAIEILEQALASTA 60
M I+++ P+ ++T Q + TY++T L+ G+ +EAIE + ALA T
Sbjct 111 MPAIEIQSRPDAFSKTI------VTQQVNTYLQTLLLFKEEGKTVSEAIEQAQTALAVTG 164
Query 61 SPGISRETIEASRRELRG 78
+S E + ++ L G
Sbjct 165 QVELSTEYSQRMKKMLTG 182
>gi|154246135|ref|YP_001417093.1| hypothetical protein Xaut_2192 [Xanthobacter autotrophicus Py2]
gi|154160220|gb|ABS67436.1| conserved hypothetical protein [Xanthobacter autotrophicus Py2]
Length=74
Score = 34.3 bits (77), Expect = 5.9, Method: Compositional matrix adjust.
Identities = 23/70 (33%), Positives = 37/70 (53%), Gaps = 2/70 (2%)
Query 1 MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEAIEILEQALAST- 59
MA + +R++P++ YR RA G SL+ +R L+E R E + + E LA T
Sbjct 1 MAQVLIRNIPDETLNVYRERAKRNGISLEQEIRN-LLERNRPYTPEERVAVSEYFLARTK 59
Query 60 ASPGISRETI 69
SP ++ + I
Sbjct 60 PSPPLTLDEI 69
Lambda K H
0.313 0.128 0.330
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 129292541152
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Sep 5, 2011 4:36 AM
Number of letters in database: 5,219,829,388
Number of sequences in database: 15,229,318
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40