BLASTP 2.2.25+
Reference:
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database
search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
15,229,318 sequences; 5,219,829,388 total letters
Query= Rv0076c
Length=129
Score E
Sequences producing significant alignments: (Bits) Value
gi|15607218|ref|NP_214590.1| hypothetical protein Rv0076c [Mycob... 265 2e-69
gi|308370307|ref|ZP_07421011.2| membrane protein [Mycobacterium ... 257 4e-67
gi|308232629|ref|ZP_07416705.2| membrane protein [Mycobacterium ... 213 9e-54
gi|183980252|ref|YP_001848543.1| hypothetical protein MMAR_0219 ... 157 4e-37
gi|240168828|ref|ZP_04747487.1| hypothetical protein MkanA1_0591... 130 5e-29
gi|41410414|ref|NP_963250.1| hypothetical protein MAP4316 [Mycob... 114 4e-24
gi|296167173|ref|ZP_06849580.1| conserved hypothetical protein [... 98.2 4e-19
gi|254777626|ref|ZP_05219142.1| hypothetical protein MaviaA2_235... 90.9 5e-17
gi|333990645|ref|YP_004523259.1| hypothetical protein JDM601_200... 83.2 1e-14
gi|118463988|ref|YP_884391.1| hypothetical protein MAV_5279 [Myc... 77.0 8e-13
gi|342862319|ref|ZP_08718960.1| hypothetical protein MCOL_25633 ... 73.6 1e-11
gi|254819426|ref|ZP_05224427.1| hypothetical protein MintA_05854... 70.9 6e-11
gi|118468606|ref|YP_884994.1| hypothetical protein MSMEG_0583 [M... 56.6 1e-06
gi|288961172|ref|YP_003451511.1| glyceraldehyde 3-phosphate dehy... 37.4 0.79
gi|288957812|ref|YP_003448153.1| glyceraldehyde 3-phosphate dehy... 37.4 0.82
gi|334338550|ref|XP_001379964.2| PREDICTED: adenylate cyclase ty... 33.9 7.3
>gi|15607218|ref|NP_214590.1| hypothetical protein Rv0076c [Mycobacterium tuberculosis H37Rv]
gi|15839455|ref|NP_334492.1| hypothetical protein MT0082 [Mycobacterium tuberculosis CDC1551]
gi|31791253|ref|NP_853746.1| hypothetical protein Mb0078c [Mycobacterium bovis AF2122/97]
64 more sequence titles
Length=129
Score = 265 bits (676), Expect = 2e-69, Method: Compositional matrix adjust.
Identities = 129/129 (100%), Positives = 129/129 (100%), Gaps = 0/129 (0%)
Query 1 MPAVTTPSNHWGDERRKLSHQPPVRGQILGRRQARRLSQHFARVGVEAPPKRLQEMLLGA 60
MPAVTTPSNHWGDERRKLSHQPPVRGQILGRRQARRLSQHFARVGVEAPPKRLQEMLLGA
Sbjct 1 MPAVTTPSNHWGDERRKLSHQPPVRGQILGRRQARRLSQHFARVGVEAPPKRLQEMLLGA 60
Query 61 PAADEEWTDVKFALIVTQLNHEKRVAKFHRLQRRATHSLICLGLVLVALNFLICLAYIFF 120
PAADEEWTDVKFALIVTQLNHEKRVAKFHRLQRRATHSLICLGLVLVALNFLICLAYIFF
Sbjct 61 PAADEEWTDVKFALIVTQLNHEKRVAKFHRLQRRATHSLICLGLVLVALNFLICLAYIFF 120
Query 121 SLTQHAAAL 129
SLTQHAAAL
Sbjct 121 SLTQHAAAL 129
>gi|308370307|ref|ZP_07421011.2| membrane protein [Mycobacterium tuberculosis SUMu003]
gi|308376299|ref|ZP_07438319.2| membrane protein [Mycobacterium tuberculosis SUMu008]
gi|308378539|ref|ZP_07482919.2| membrane protein [Mycobacterium tuberculosis SUMu009]
gi|308332454|gb|EFP21305.1| membrane protein [Mycobacterium tuberculosis SUMu003]
gi|308351552|gb|EFP40403.1| membrane protein [Mycobacterium tuberculosis SUMu008]
gi|308352237|gb|EFP41088.1| membrane protein [Mycobacterium tuberculosis SUMu009]
Length=126
Score = 257 bits (657), Expect = 4e-67, Method: Compositional matrix adjust.
Identities = 125/126 (99%), Positives = 126/126 (100%), Gaps = 0/126 (0%)
Query 4 VTTPSNHWGDERRKLSHQPPVRGQILGRRQARRLSQHFARVGVEAPPKRLQEMLLGAPAA 63
+TTPSNHWGDERRKLSHQPPVRGQILGRRQARRLSQHFARVGVEAPPKRLQEMLLGAPAA
Sbjct 1 MTTPSNHWGDERRKLSHQPPVRGQILGRRQARRLSQHFARVGVEAPPKRLQEMLLGAPAA 60
Query 64 DEEWTDVKFALIVTQLNHEKRVAKFHRLQRRATHSLICLGLVLVALNFLICLAYIFFSLT 123
DEEWTDVKFALIVTQLNHEKRVAKFHRLQRRATHSLICLGLVLVALNFLICLAYIFFSLT
Sbjct 61 DEEWTDVKFALIVTQLNHEKRVAKFHRLQRRATHSLICLGLVLVALNFLICLAYIFFSLT 120
Query 124 QHAAAL 129
QHAAAL
Sbjct 121 QHAAAL 126
>gi|308232629|ref|ZP_07416705.2| membrane protein [Mycobacterium tuberculosis SUMu001]
gi|308371575|ref|ZP_07425377.2| membrane protein [Mycobacterium tuberculosis SUMu004]
gi|308372800|ref|ZP_07429915.2| membrane protein [Mycobacterium tuberculosis SUMu005]
gi|308373880|ref|ZP_07433978.2| membrane protein [Mycobacterium tuberculosis SUMu006]
gi|308213360|gb|EFO72759.1| membrane protein [Mycobacterium tuberculosis SUMu001]
gi|308336243|gb|EFP25094.1| membrane protein [Mycobacterium tuberculosis SUMu004]
gi|308339832|gb|EFP28683.1| membrane protein [Mycobacterium tuberculosis SUMu005]
gi|308343826|gb|EFP32677.1| membrane protein [Mycobacterium tuberculosis SUMu006]
Length=106
Score = 213 bits (541), Expect = 9e-54, Method: Compositional matrix adjust.
Identities = 105/106 (99%), Positives = 106/106 (100%), Gaps = 0/106 (0%)
Query 24 VRGQILGRRQARRLSQHFARVGVEAPPKRLQEMLLGAPAADEEWTDVKFALIVTQLNHEK 83
+RGQILGRRQARRLSQHFARVGVEAPPKRLQEMLLGAPAADEEWTDVKFALIVTQLNHEK
Sbjct 1 MRGQILGRRQARRLSQHFARVGVEAPPKRLQEMLLGAPAADEEWTDVKFALIVTQLNHEK 60
Query 84 RVAKFHRLQRRATHSLICLGLVLVALNFLICLAYIFFSLTQHAAAL 129
RVAKFHRLQRRATHSLICLGLVLVALNFLICLAYIFFSLTQHAAAL
Sbjct 61 RVAKFHRLQRRATHSLICLGLVLVALNFLICLAYIFFSLTQHAAAL 106
>gi|183980252|ref|YP_001848543.1| hypothetical protein MMAR_0219 [Mycobacterium marinum M]
gi|183173578|gb|ACC38688.1| conserved hypothetical membrane protein [Mycobacterium marinum
M]
Length=125
Score = 157 bits (398), Expect = 4e-37, Method: Compositional matrix adjust.
Identities = 73/120 (61%), Positives = 92/120 (77%), Gaps = 0/120 (0%)
Query 4 VTTPSNHWGDERRKLSHQPPVRGQILGRRQARRLSQHFARVGVEAPPKRLQEMLLGAPAA 63
+TT SN WGDERR+LSHQ P+R + GR QARRLS+ FA VGV P+RL+EM G PA+
Sbjct 1 MTTSSNPWGDERRRLSHQLPMRDHLFGRSQARRLSRRFAGVGVRISPQRLREMHAGMPAS 60
Query 64 DEEWTDVKFALIVTQLNHEKRVAKFHRLQRRATHSLICLGLVLVALNFLICLAYIFFSLT 123
D E T++ FALI Q+ EKRVAK HRLQR+ H+L+ LG+V+VALNFL+C+ Y+ FSLT
Sbjct 61 DSEMTNINFALIAIQITREKRVAKLHRLQRKCRHALLSLGMVIVALNFLLCIGYLLFSLT 120
>gi|240168828|ref|ZP_04747487.1| hypothetical protein MkanA1_05915 [Mycobacterium kansasii ATCC
12478]
Length=106
Score = 130 bits (328), Expect = 5e-29, Method: Compositional matrix adjust.
Identities = 62/105 (60%), Positives = 78/105 (75%), Gaps = 0/105 (0%)
Query 24 VRGQILGRRQARRLSQHFARVGVEAPPKRLQEMLLGAPAADEEWTDVKFALIVTQLNHEK 83
+R GR QARRLS+ FARVGVE P RL+EML+G P +D+E T V FALI ++NHE
Sbjct 1 MRDHFFGRHQARRLSRRFARVGVEIPSGRLREMLVGMPVSDDEMTSVSFALIAIRINHEN 60
Query 84 RVAKFHRLQRRATHSLICLGLVLVALNFLICLAYIFFSLTQHAAA 128
RVAK RLQRR +LI +GL LVALNFL+C+ Y+F +LTQ A++
Sbjct 61 RVAKVRRLQRRCRQALISVGLALVALNFLLCIGYLFLTLTQQASS 105
>gi|41410414|ref|NP_963250.1| hypothetical protein MAP4316 [Mycobacterium avium subsp. paratuberculosis
K-10]
gi|41399248|gb|AAS06866.1| hypothetical protein MAP_4316 [Mycobacterium avium subsp. paratuberculosis
K-10]
Length=126
Score = 114 bits (285), Expect = 4e-24, Method: Compositional matrix adjust.
Identities = 63/126 (50%), Positives = 85/126 (68%), Gaps = 0/126 (0%)
Query 4 VTTPSNHWGDERRKLSHQPPVRGQILGRRQARRLSQHFARVGVEAPPKRLQEMLLGAPAA 63
+ TPS WGD+R +L+ Q +R L RR+ARRL F RVGV P+RL+EML GAP A
Sbjct 1 MMTPSYPWGDDRGQLAQQLRLRYHTLRRRRARRLCHSFERVGVRTAPERLREMLAGAPLA 60
Query 64 DEEWTDVKFALIVTQLNHEKRVAKFHRLQRRATHSLICLGLVLVALNFLICLAYIFFSLT 123
E +V FALI QLN E RVA++ RL++R SL+ GL++V +NFL+C+AY+ +L
Sbjct 61 AHEVINVNFALIAIQLNREARVARYRRLRQRGARSLMFAGLIMVVINFLMCMAYLLLNLA 120
Query 124 QHAAAL 129
+ AA L
Sbjct 121 EPAAPL 126
>gi|296167173|ref|ZP_06849580.1| conserved hypothetical protein [Mycobacterium parascrofulaceum
ATCC BAA-614]
gi|295897495|gb|EFG77094.1| conserved hypothetical protein [Mycobacterium parascrofulaceum
ATCC BAA-614]
Length=93
Score = 98.2 bits (243), Expect = 4e-19, Method: Compositional matrix adjust.
Identities = 47/93 (51%), Positives = 63/93 (68%), Gaps = 0/93 (0%)
Query 37 LSQHFARVGVEAPPKRLQEMLLGAPAADEEWTDVKFALIVTQLNHEKRVAKFHRLQRRAT 96
+ Q+F RVGV P R+QEML GAP A E DV FALI +L+ E R A++ RL+R+ T
Sbjct 1 MCQNFERVGVRTAPDRVQEMLAGAPLAAHEVVDVHFALIALRLDRETRAARYKRLKRQGT 60
Query 97 HSLICLGLVLVALNFLICLAYIFFSLTQHAAAL 129
S++ GLVLV LNFL+C+AY+ +L + A L
Sbjct 61 RSMMFAGLVLVVLNFLMCMAYLLLNLAEQGAPL 93
>gi|254777626|ref|ZP_05219142.1| hypothetical protein MaviaA2_23556 [Mycobacterium avium subsp.
avium ATCC 25291]
Length=86
Score = 90.9 bits (224), Expect = 5e-17, Method: Compositional matrix adjust.
Identities = 43/86 (50%), Positives = 60/86 (70%), Gaps = 0/86 (0%)
Query 44 VGVEAPPKRLQEMLLGAPAADEEWTDVKFALIVTQLNHEKRVAKFHRLQRRATHSLICLG 103
+GV P+RL+EML GAP A E +V FALI QLN E RVA++ RL++R SL+ G
Sbjct 1 MGVRTAPERLREMLAGAPLAAHEVINVNFALIAIQLNREARVARYRRLRQRGARSLMFAG 60
Query 104 LVLVALNFLICLAYIFFSLTQHAAAL 129
L++V +NFL+C+AY+ +L + AA L
Sbjct 61 LIMVVINFLMCMAYLLLNLAEPAAPL 86
>gi|333990645|ref|YP_004523259.1| hypothetical protein JDM601_2005 [Mycobacterium sp. JDM601]
gi|333486613|gb|AEF36005.1| conserved hypothetical protein [Mycobacterium sp. JDM601]
Length=101
Score = 83.2 bits (204), Expect = 1e-14, Method: Compositional matrix adjust.
Identities = 49/101 (49%), Positives = 63/101 (63%), Gaps = 0/101 (0%)
Query 29 LGRRQARRLSQHFARVGVEAPPKRLQEMLLGAPAADEEWTDVKFALIVTQLNHEKRVAKF 88
+GR ARR+S+ FA VGV+ P RL+EM+ GAP A E D FAL T + E+R+A+
Sbjct 1 MGRWGARRMSRRFAGVGVQIPAARLREMMAGAPPASAESIDYTFALAATAIQREQRLARA 60
Query 89 HRLQRRATHSLICLGLVLVALNFLICLAYIFFSLTQHAAAL 129
R +RR LI GL L ALN LIC+ Y+F SL H A+
Sbjct 61 RRNRRRLVQLLIIAGLTLAALNLLICMGYLFVSLALHEPAM 101
>gi|118463988|ref|YP_884391.1| hypothetical protein MAV_5279 [Mycobacterium avium 104]
gi|118165275|gb|ABK66172.1| conserved hypothetical protein [Mycobacterium avium 104]
gi|336459782|gb|EGO38696.1| hypothetical protein MAPs_46730 [Mycobacterium avium subsp. paratuberculosis
S397]
Length=74
Score = 77.0 bits (188), Expect = 8e-13, Method: Compositional matrix adjust.
Identities = 37/74 (50%), Positives = 51/74 (69%), Gaps = 0/74 (0%)
Query 56 MLLGAPAADEEWTDVKFALIVTQLNHEKRVAKFHRLQRRATHSLICLGLVLVALNFLICL 115
ML GAP A E +V FALI QLN E RVA++ RL++R SL+ GL++V +NFL+C+
Sbjct 1 MLAGAPLAAHEVINVNFALIAIQLNREARVARYRRLRQRGARSLMFAGLIMVVINFLMCM 60
Query 116 AYIFFSLTQHAAAL 129
AY+ +L + AA L
Sbjct 61 AYLLLNLAEPAAPL 74
>gi|342862319|ref|ZP_08718960.1| hypothetical protein MCOL_25633 [Mycobacterium colombiense CECT
3035]
gi|342130176|gb|EGT83504.1| hypothetical protein MCOL_25633 [Mycobacterium colombiense CECT
3035]
Length=77
Score = 73.6 bits (179), Expect = 1e-11, Method: Compositional matrix adjust.
Identities = 34/77 (45%), Positives = 52/77 (68%), Gaps = 0/77 (0%)
Query 53 LQEMLLGAPAADEEWTDVKFALIVTQLNHEKRVAKFHRLQRRATHSLICLGLVLVALNFL 112
+++ML GAP E +V FALI +L+ E R A++ RL++R SL+ GLV+V LNFL
Sbjct 1 MRQMLEGAPLGAHELMNVNFALIAIRLDRELRAARYKRLKQRGARSLMFAGLVMVVLNFL 60
Query 113 ICLAYIFFSLTQHAAAL 129
+C+AY+ +L + +A L
Sbjct 61 VCMAYLLLNLAEQSAPL 77
>gi|254819426|ref|ZP_05224427.1| hypothetical protein MintA_05854 [Mycobacterium intracellulare
ATCC 13950]
Length=74
Score = 70.9 bits (172), Expect = 6e-11, Method: Compositional matrix adjust.
Identities = 34/74 (46%), Positives = 49/74 (67%), Gaps = 0/74 (0%)
Query 56 MLLGAPAADEEWTDVKFALIVTQLNHEKRVAKFHRLQRRATHSLICLGLVLVALNFLICL 115
ML GAP A E ++ FALI +L+ E R A++ RL++R SL+ GLV+V LNFL+C+
Sbjct 1 MLEGAPLAAHEMINLNFALIAIRLDRESRAARYKRLRQRGARSLMFAGLVMVVLNFLVCM 60
Query 116 AYIFFSLTQHAAAL 129
AY+ +L + A L
Sbjct 61 AYLLLNLAEPTAPL 74
>gi|118468606|ref|YP_884994.1| hypothetical protein MSMEG_0583 [Mycobacterium smegmatis str.
MC2 155]
gi|118169893|gb|ABK70789.1| probable membrane protein [Mycobacterium smegmatis str. MC2 155]
Length=86
Score = 56.6 bits (135), Expect = 1e-06, Method: Compositional matrix adjust.
Identities = 29/83 (35%), Positives = 49/83 (60%), Gaps = 0/83 (0%)
Query 44 VGVEAPPKRLQEMLLGAPAADEEWTDVKFALIVTQLNHEKRVAKFHRLQRRATHSLICLG 103
+GV RL+E+ GAPAA E DV+FA + T+L H++++A+ R +R LI +
Sbjct 1 MGVGIAATRLRELADGAPAAAGELADVEFAFVATELRHDEQLARIRRGKRACVSCLITVA 60
Query 104 LVLVALNFLICLAYIFFSLTQHA 126
+ ++ + LI + + FSL H+
Sbjct 61 VGVLMIVSLISMVLLLFSLILHS 83
>gi|288961172|ref|YP_003451511.1| glyceraldehyde 3-phosphate dehydrogenase [Azospirillum sp. B510]
gi|288913480|dbj|BAI74967.1| glyceraldehyde 3-phosphate dehydrogenase [Azospirillum sp. B510]
Length=335
Score = 37.4 bits (85), Expect = 0.79, Method: Compositional matrix adjust.
Identities = 22/68 (33%), Positives = 37/68 (55%), Gaps = 7/68 (10%)
Query 54 QEMLLGAPAADEEWTDVKFALIVTQLNHEKRVAKFHRLQRRATHSLICLGLVLVALNFLI 113
Q++L+ APA DE+ T +V +NH+K A+ H++ A+ + CL V LN L+
Sbjct 117 QKVLISAPATDEDIT------VVYGVNHDKLTAE-HKIVSNASCTTNCLAPVAFVLNNLV 169
Query 114 CLAYIFFS 121
+ F +
Sbjct 170 GIEKGFMT 177
>gi|288957812|ref|YP_003448153.1| glyceraldehyde 3-phosphate dehydrogenase [Azospirillum sp. B510]
gi|288910120|dbj|BAI71609.1| glyceraldehyde 3-phosphate dehydrogenase [Azospirillum sp. B510]
Length=335
Score = 37.4 bits (85), Expect = 0.82, Method: Compositional matrix adjust.
Identities = 22/68 (33%), Positives = 37/68 (55%), Gaps = 7/68 (10%)
Query 54 QEMLLGAPAADEEWTDVKFALIVTQLNHEKRVAKFHRLQRRATHSLICLGLVLVALNFLI 113
Q++L+ APA DE+ T +V +NH+K A+ H++ A+ + CL V LN L+
Sbjct 117 QKVLISAPATDEDIT------VVYGVNHDKLTAE-HKIVSNASCTTNCLAPVAFVLNNLV 169
Query 114 CLAYIFFS 121
+ F +
Sbjct 170 GIEKGFMT 177
>gi|334338550|ref|XP_001379964.2| PREDICTED: adenylate cyclase type 1-like [Monodelphis domestica]
Length=1094
Score = 33.9 bits (76), Expect = 7.3, Method: Composition-based stats.
Identities = 23/67 (35%), Positives = 32/67 (48%), Gaps = 2/67 (2%)
Query 52 RLQEMLLGAPAADEEWTDVKFALIVTQLNHEKRVAKFHRLQRRATHSLICLGLVLVALNF 111
R L+ A + E D+ F Q H++R K+H+LQ S + L LVL AL
Sbjct 559 RYISRLIEARQTESEMADLNF--FTLQYKHKEREEKYHQLQDEYFTSAVVLSLVLAALFG 616
Query 112 LICLAYI 118
L+ L I
Sbjct 617 LVYLLII 623
Lambda K H
0.327 0.137 0.421
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 128283502052
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Sep 5, 2011 4:36 AM
Number of letters in database: 5,219,829,388
Number of sequences in database: 15,229,318
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40