BLASTP 2.2.25+


Reference:
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database
search programs", Nucleic Acids Res. 25:3389-3402.



Reference for composition-based statistics:
Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.



Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
           15,229,318 sequences; 5,219,829,388 total letters



Query= Rv0100

Length=78
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

gi|254233495|ref|ZP_04926821.1|  conserved hypothetical protein [...   155    2e-36
gi|15607242|ref|NP_214614.1|  hypothetical protein Rv0100 [Mycoba...   154    4e-36
gi|340625136|ref|YP_004743588.1|  hypothetical protein MCAN_01031...   152    1e-35
gi|183980289|ref|YP_001848580.1|  hypothetical protein MMAR_0257 ...   113    8e-24
gi|118619897|ref|YP_908229.1|  hypothetical protein MUL_4857 [Myc...   111    3e-23
gi|15828083|ref|NP_302346.1|  hypothetical protein ML1995 [Mycoba...   106    1e-21
gi|317506389|ref|ZP_07964198.1|  hypothetical protein HMPREF9336_...  92.0    2e-17
gi|169627765|ref|YP_001701414.1|  hypothetical protein MAB_0662 [...  90.5    8e-17
gi|169629210|ref|YP_001702859.1|  putative phenyloxazoline syntha...  36.6    1.3  
gi|226306183|ref|YP_002766143.1|  hypothetical protein RER_26960 ...  36.2    1.7  
gi|158314872|ref|YP_001507380.1|  phosphopantetheine-binding prot...  35.4    3.0  
gi|298293285|ref|YP_003695224.1|  phosphopantetheine-binding prot...  34.7    4.4  
gi|117168626|gb|ABK32290.1|  JerD [Sorangium cellulosum]              34.7    4.6  
gi|345012844|ref|YP_004815198.1|  beta-ketoacyl synthase [Strepto...  34.7    5.0  
gi|229918248|ref|YP_002886894.1|  beta-lactamase domain protein [...  34.3    6.1  
gi|345011738|ref|YP_004814092.1|  acyl transferase [Streptomyces ...  34.3    6.8  
gi|20150009|gb|AAM12911.1|  MmpII [Pseudomonas fluorescens]           34.3    7.0  
gi|37521510|ref|NP_924887.1|  polyketide synthase [Gloeobacter vi...  34.3    7.2  
gi|308174884|ref|YP_003921589.1|  isochorismatase [Bacillus amylo...  33.9    8.3  
gi|328554838|gb|AEB25330.1|  isochorismatase [Bacillus amylolique...  33.9    8.7  
gi|305665878|ref|YP_003862165.1|  putative thymidine kinase [Mari...  33.5    9.9  


>gi|254233495|ref|ZP_04926821.1| conserved hypothetical protein [Mycobacterium tuberculosis C]
 gi|308231462|ref|ZP_07663864.1| putative phosphopantetheine attachment site [Mycobacterium tuberculosis 
SUMu001]
 gi|308372803|ref|ZP_07429942.2| hypothetical protein TMEG_00526 [Mycobacterium tuberculosis SUMu005]
 11 more sequence titles
 Length=82

 Score =  155 bits (392),  Expect = 2e-36, Method: Compositional matrix adjust.
 Identities = 78/78 (100%), Positives = 78/78 (100%), Gaps = 0/78 (0%)

Query  1   VRDRILAAVCDVLYIDEADLIDGDETDLRDLGLDSVRFVLLMKQLGVNRQSELPSRLAAN  60
           VRDRILAAVCDVLYIDEADLIDGDETDLRDLGLDSVRFVLLMKQLGVNRQSELPSRLAAN
Sbjct  5   VRDRILAAVCDVLYIDEADLIDGDETDLRDLGLDSVRFVLLMKQLGVNRQSELPSRLAAN  64

Query  61  PSIAGWLRELEAVCTEFG  78
           PSIAGWLRELEAVCTEFG
Sbjct  65  PSIAGWLRELEAVCTEFG  82


>gi|15607242|ref|NP_214614.1| hypothetical protein Rv0100 [Mycobacterium tuberculosis H37Rv]
 gi|15839480|ref|NP_334517.1| hypothetical protein MT0109 [Mycobacterium tuberculosis CDC1551]
 gi|31791278|ref|NP_853771.1| hypothetical protein Mb0103 [Mycobacterium bovis AF2122/97]
 62 more sequence titles
 Length=78

 Score =  154 bits (389),  Expect = 4e-36, Method: Compositional matrix adjust.
 Identities = 77/78 (99%), Positives = 78/78 (100%), Gaps = 0/78 (0%)

Query  1   VRDRILAAVCDVLYIDEADLIDGDETDLRDLGLDSVRFVLLMKQLGVNRQSELPSRLAAN  60
           +RDRILAAVCDVLYIDEADLIDGDETDLRDLGLDSVRFVLLMKQLGVNRQSELPSRLAAN
Sbjct  1   MRDRILAAVCDVLYIDEADLIDGDETDLRDLGLDSVRFVLLMKQLGVNRQSELPSRLAAN  60

Query  61  PSIAGWLRELEAVCTEFG  78
           PSIAGWLRELEAVCTEFG
Sbjct  61  PSIAGWLRELEAVCTEFG  78


>gi|340625136|ref|YP_004743588.1| hypothetical protein MCAN_01031 [Mycobacterium canettii CIPT 
140010059]
 gi|340003326|emb|CCC42444.1| conserved hypothetical protein [Mycobacterium canettii CIPT 140010059]
Length=78

 Score =  152 bits (385),  Expect = 1e-35, Method: Compositional matrix adjust.
 Identities = 76/78 (98%), Positives = 77/78 (99%), Gaps = 0/78 (0%)

Query  1   VRDRILAAVCDVLYIDEADLIDGDETDLRDLGLDSVRFVLLMKQLGVNRQSELPSRLAAN  60
           +RDRILAAVCDVLYIDEADLIDGDETDLRDLGLDSVRFVLLMKQLGVNRQSELPSRLAAN
Sbjct  1   MRDRILAAVCDVLYIDEADLIDGDETDLRDLGLDSVRFVLLMKQLGVNRQSELPSRLAAN  60

Query  61  PSIAGWLRELEAVCTEFG  78
           PSIAGWLRELEA CTEFG
Sbjct  61  PSIAGWLRELEAACTEFG  78


>gi|183980289|ref|YP_001848580.1| hypothetical protein MMAR_0257 [Mycobacterium marinum M]
 gi|183173615|gb|ACC38725.1| conserved hypothetical protein [Mycobacterium marinum M]
Length=83

 Score =  113 bits (283),  Expect = 8e-24, Method: Compositional matrix adjust.
 Identities = 58/70 (83%), Positives = 62/70 (89%), Gaps = 0/70 (0%)

Query  1   VRDRILAAVCDVLYIDEADLIDGDETDLRDLGLDSVRFVLLMKQLGVNRQSELPSRLAAN  60
           VR RIL AVCDVLYIDE DLIDGD TDLRDLGLDSVRFVLLMK+LGV+R+SELPSRLA N
Sbjct  5   VRQRILLAVCDVLYIDETDLIDGDATDLRDLGLDSVRFVLLMKRLGVDRESELPSRLAEN  64

Query  61  PSIAGWLREL  70
            SI GW+ EL
Sbjct  65  LSIEGWVSEL  74


>gi|118619897|ref|YP_908229.1| hypothetical protein MUL_4857 [Mycobacterium ulcerans Agy99]
 gi|118572007|gb|ABL06758.1| conserved hypothetical protein [Mycobacterium ulcerans Agy99]
Length=79

 Score =  111 bits (278),  Expect = 3e-23, Method: Compositional matrix adjust.
 Identities = 56/70 (80%), Positives = 62/70 (89%), Gaps = 0/70 (0%)

Query  1   VRDRILAAVCDVLYIDEADLIDGDETDLRDLGLDSVRFVLLMKQLGVNRQSELPSRLAAN  60
           +R+RIL A CDVLYIDE DLIDGD TDLRDLGLDSVRFVLLMK+LGV+R+SELPSRLA N
Sbjct  1   MRERILLAACDVLYIDETDLIDGDATDLRDLGLDSVRFVLLMKRLGVDRESELPSRLAEN  60

Query  61  PSIAGWLREL  70
            SI GW+ EL
Sbjct  61  LSIEGWVSEL  70


>gi|15828083|ref|NP_302346.1| hypothetical protein ML1995 [Mycobacterium leprae TN]
 gi|221230560|ref|YP_002503976.1| hypothetical protein MLBr_01995 [Mycobacterium leprae Br4923]
 gi|13093637|emb|CAC30950.1| conserved hypothetical protein [Mycobacterium leprae]
 gi|219933667|emb|CAR72092.1| conserved hypothetical protein [Mycobacterium leprae Br4923]
Length=78

 Score =  106 bits (264),  Expect = 1e-21, Method: Compositional matrix adjust.
 Identities = 49/71 (70%), Positives = 63/71 (89%), Gaps = 0/71 (0%)

Query  2   RDRILAAVCDVLYIDEADLIDGDETDLRDLGLDSVRFVLLMKQLGVNRQSELPSRLAANP  61
           R+RIL AVC+VLYI E+DL+DGDETDLRDLGLDSVRF LLMKQLG+++++E+ S+L  N 
Sbjct  6   RERILTAVCEVLYIAESDLVDGDETDLRDLGLDSVRFTLLMKQLGLSQEAEMQSKLMDNF  65

Query  62  SIAGWLRELEA  72
           SIA W+R+LE+
Sbjct  66  SIANWVRQLES  76


>gi|317506389|ref|ZP_07964198.1| hypothetical protein HMPREF9336_00568 [Segniliparus rugosus ATCC 
BAA-974]
 gi|316255318|gb|EFV14579.1| hypothetical protein HMPREF9336_00568 [Segniliparus rugosus ATCC 
BAA-974]
Length=79

 Score = 92.0 bits (227),  Expect = 2e-17, Method: Compositional matrix adjust.
 Identities = 45/66 (69%), Positives = 58/66 (88%), Gaps = 0/66 (0%)

Query  1   VRDRILAAVCDVLYIDEADLIDGDETDLRDLGLDSVRFVLLMKQLGVNRQSELPSRLAAN  60
           +R+++LAA+C+VLYI E DL+DGD TDLRDLGLDSVRFVLLM++LGV+R S+L SRL  +
Sbjct  1   MREKVLAALCEVLYIAEDDLVDGDATDLRDLGLDSVRFVLLMQRLGVDRGSDLWSRLGES  60

Query  61  PSIAGW  66
            S+AGW
Sbjct  61  LSVAGW  66


>gi|169627765|ref|YP_001701414.1| hypothetical protein MAB_0662 [Mycobacterium abscessus ATCC 19977]
 gi|169239732|emb|CAM60760.1| Conserved hypothetical protein [Mycobacterium abscessus]
Length=71

 Score = 90.5 bits (223),  Expect = 8e-17, Method: Compositional matrix adjust.
 Identities = 44/63 (70%), Positives = 52/63 (83%), Gaps = 0/63 (0%)

Query  9   VCDVLYIDEADLIDGDETDLRDLGLDSVRFVLLMKQLGVNRQSELPSRLAANPSIAGWLR  68
           +C+VLYI EADL +GD TDLR+LGLDSVRFVLLMKQLGV R SEL  RL ++ S+AGW +
Sbjct  1   MCEVLYISEADLFEGDSTDLRELGLDSVRFVLLMKQLGVTRGSELQKRLVSDLSVAGWAQ  60

Query  69  ELE  71
            LE
Sbjct  61  ILE  63


>gi|169629210|ref|YP_001702859.1| putative phenyloxazoline synthase MbtB [Mycobacterium abscessus 
ATCC 19977]
 gi|169241177|emb|CAM62205.1| Putative phenyloxazoline synthase MbtB [Mycobacterium abscessus]
Length=1155

 Score = 36.6 bits (83),  Expect = 1.3, Method: Compositional matrix adjust.
 Identities = 26/71 (37%), Positives = 35/71 (50%), Gaps = 2/71 (2%)

Query  2   RDRILAAVCDVLYIDEADLIDGDETDLRDLGLDSVRFVLLMKQLGVNRQSELPSRLAANP  61
           RD I   V D++ +   D+ DGD  DL  LGLDS+R + L             ++LAA P
Sbjct  6   RDEIKLTVADLIGLTAQDISDGD--DLITLGLDSIRMMTLAGGWRKRGSRVTFAQLAAEP  63

Query  62  SIAGWLRELEA  72
           S+  W   L A
Sbjct  64  SVDSWYALLRA  74


>gi|226306183|ref|YP_002766143.1| hypothetical protein RER_26960 [Rhodococcus erythropolis PR4]
 gi|229490894|ref|ZP_04384729.1| isochorismatase [Rhodococcus erythropolis SK121]
 gi|226185300|dbj|BAH33404.1| hypothetical protein RER_26960 [Rhodococcus erythropolis PR4]
 gi|229322284|gb|EEN88070.1| isochorismatase [Rhodococcus erythropolis SK121]
Length=76

 Score = 36.2 bits (82),  Expect = 1.7, Method: Compositional matrix adjust.
 Identities = 26/70 (38%), Positives = 41/70 (59%), Gaps = 4/70 (5%)

Query  2   RDRILAAVCDVLYIDEADLIDGDETDLRDLGLDSVRFVLLMKQL-GVNRQSELPSRLAAN  60
           RD+I+  V +VL +    L  GD+ DL D GLDSVR + L+++  G    ++  + LA  
Sbjct  7   RDQIVRDVAEVLSMRAEQL--GDDADLGDEGLDSVRLMTLVERWRGAGADADF-AELAEE  63

Query  61  PSIAGWLREL  70
           P+I  W++ L
Sbjct  64  PTINAWVQVL  73


>gi|158314872|ref|YP_001507380.1| phosphopantetheine-binding protein [Frankia sp. EAN1pec]
 gi|158110277|gb|ABW12474.1| phosphopantetheine-binding [Frankia sp. EAN1pec]
Length=84

 Score = 35.4 bits (80),  Expect = 3.0, Method: Compositional matrix adjust.
 Identities = 22/42 (53%), Positives = 31/42 (74%), Gaps = 2/42 (4%)

Query  3   DRILAAVCDVLYIDEADLIDGDETDLRDLGLDSVRFVLLMKQ  44
           +R+LA V DVLYI EA+ +D D  DLR  G+DSVR + L+++
Sbjct  6   NRVLADVADVLYI-EANDVDQD-ADLRAQGMDSVRLMELVER  45


>gi|298293285|ref|YP_003695224.1| phosphopantetheine-binding protein [Starkeya novella DSM 506]
 gi|296929796|gb|ADH90605.1| phosphopantetheine-binding protein [Starkeya novella DSM 506]
Length=95

 Score = 34.7 bits (78),  Expect = 4.4, Method: Compositional matrix adjust.
 Identities = 25/69 (37%), Positives = 41/69 (60%), Gaps = 8/69 (11%)

Query  1   VRDRILAAVCDVLYIDEADLIDGDETDLRDLGLDSVR---FVLLMKQLGVNRQSELPSRL  57
            R+++ A +  +L+ D  + IDG+++ L DLGLDS+R    VLL  + G+  +    S  
Sbjct  19  TREKMRADIAKMLHEDPEE-IDGEDS-LIDLGLDSMRAMNLVLLWSEGGLELEF---SEF  73

Query  58  AANPSIAGW  66
           A NP++ GW
Sbjct  74  AENPTLDGW  82


>gi|117168626|gb|ABK32290.1| JerD [Sorangium cellulosum]
Length=1880

 Score = 34.7 bits (78),  Expect = 4.6, Method: Compositional matrix adjust.
 Identities = 26/74 (36%), Positives = 39/74 (53%), Gaps = 5/74 (6%)

Query  9     VCDVLYIDEADLIDGDETDLRDLGLDSVRFVLLMKQLGVNRQSELPSRLA---ANP-SIA  64
             V +VL +     +  D+  LRDLGLDS+  V L  +LG   ++ LP+ LA     P ++A
Sbjct  1730  VAEVLQLPGPAHVPADQ-PLRDLGLDSLMTVELRNRLGARAETTLPTTLAFDYPTPRALA  1788

Query  65    GWLRELEAVCTEFG  78
              +L  L  +  E G
Sbjct  1789  SYLETLLGISDENG  1802


>gi|345012844|ref|YP_004815198.1| beta-ketoacyl synthase [Streptomyces violaceusniger Tu 4113]
 gi|344039193|gb|AEM84918.1| Beta-ketoacyl synthase [Streptomyces violaceusniger Tu 4113]
Length=5479

 Score = 34.7 bits (78),  Expect = 5.0, Method: Compositional matrix adjust.
 Identities = 24/58 (42%), Positives = 33/58 (57%), Gaps = 1/58 (1%)

Query  1     VRDRILAAVCDVLYIDEADLIDGDETDLRDLGLDSVRFVLLMKQLGVNRQSELPSRLA  58
             V D +L++   VL  D+A  IDG E   +DLG DS+  V L  +L     +ELP+ LA
Sbjct  3766  VLDVVLSSAAAVLGHDDASAIDG-ERAFQDLGFDSLNVVRLRNRLRELTGAELPTTLA  3822


>gi|229918248|ref|YP_002886894.1| beta-lactamase domain protein [Exiguobacterium sp. AT1b]
 gi|229469677|gb|ACQ71449.1| beta-lactamase domain protein [Exiguobacterium sp. AT1b]
Length=466

 Score = 34.3 bits (77),  Expect = 6.1, Method: Composition-based stats.
 Identities = 15/42 (36%), Positives = 28/42 (67%), Gaps = 0/42 (0%)

Query  11   DVLYIDEADLIDGDETDLRDLGLDSVRFVLLMKQLGVNRQSE  52
            D+  I  AD +D  +TDL+ +GLD +RF++ +++L  +  +E
Sbjct  321  DITLIASADDVDQVQTDLQSIGLDRLRFIVPVEELDASLLTE  362


>gi|345011738|ref|YP_004814092.1| acyl transferase [Streptomyces violaceusniger Tu 4113]
 gi|344038087|gb|AEM83812.1| Acyl transferase [Streptomyces violaceusniger Tu 4113]
Length=3456

 Score = 34.3 bits (77),  Expect = 6.8, Method: Compositional matrix adjust.
 Identities = 22/57 (39%), Positives = 28/57 (50%), Gaps = 1/57 (1%)

Query  1     VRDRILAAVCDVLYIDEADLIDGDETDLRDLGLDSVRFVLLMKQLGVNRQSELPSRL  57
             +RD + A   DVL  D AD +  D    RDLG DS+  V L  ++G      LP  L
Sbjct  1503  LRDLVRAQAADVLAHDSADAVASDRA-FRDLGFDSLTAVELRNRIGTATGLRLPVSL  1558


>gi|20150009|gb|AAM12911.1| MmpII [Pseudomonas fluorescens]
Length=2076

 Score = 34.3 bits (77),  Expect = 7.0, Method: Compositional matrix adjust.
 Identities = 24/71 (34%), Positives = 37/71 (53%), Gaps = 3/71 (4%)

Query  3     DRILAAVCDVLYIDEADLIDGDETDLRDLGLDSVRFVLLMKQLGVNRQSEL-PSRLAANP  61
             D ++A VC +L     D ID   TDL D G DSV    L+ Q+      EL P  +  + 
Sbjct  1544  DELVALVCQLLKTVAGD-ID-PHTDLHDFGFDSVLLTQLLAQISSTYGVELDPGSVLEDA  1601

Query  62    SIAGWLRELEA  72
             ++AG + +++A
Sbjct  1602  TVAGLVAQVQA  1612


>gi|37521510|ref|NP_924887.1| polyketide synthase [Gloeobacter violaceus PCC 7421]
 gi|35212507|dbj|BAC89882.1| gll1941 [Gloeobacter violaceus PCC 7421]
Length=963

 Score = 34.3 bits (77),  Expect = 7.2, Method: Compositional matrix adjust.
 Identities = 28/67 (42%), Positives = 34/67 (51%), Gaps = 6/67 (8%)

Query  2    RDRILA----AVCDVLYIDEADLIDGDETDLRDLGLDSVRFVLLMKQLGVNRQSEL-PSR  56
            RDR+ A     V DVL +  A   +  E  L DLGLDS+  V L      + Q  L P+R
Sbjct  626  RDRLAAYVQKQVMDVLKLHSAQTFE-PERSLSDLGLDSLMLVELRDHFTRDLQVTLSPAR  684

Query  57   LAANPSI  63
            L  NPSI
Sbjct  685  LLRNPSI  691


>gi|308174884|ref|YP_003921589.1| isochorismatase [Bacillus amyloliquefaciens DSM 7]
 gi|307607748|emb|CBI44119.1| isochorismatase [Bacillus amyloliquefaciens DSM 7]
 gi|328913200|gb|AEB64796.1| isochorismatase [Bacillus amyloliquefaciens LL3]
Length=306

 Score = 33.9 bits (76),  Expect = 8.3, Method: Compositional matrix adjust.
 Identities = 22/72 (31%), Positives = 38/72 (53%), Gaps = 2/72 (2%)

Query  1    VRDRILAAVCDVLYIDEADLIDGDETDLRDLGLDSVRFVLLMKQLGVNRQSELPSRLAAN  60
             ++R+   +  +L    +D++D  + DL D GLDSVR + L++Q   +        LA N
Sbjct  225  TKERMREQIAAILQESPSDILD--QEDLLDRGLDSVRIMSLVEQWRRDGAEVTFVELAEN  282

Query  61   PSIAGWLRELEA  72
            P++  W R L +
Sbjct  283  PTLEEWWRLLSS  294


>gi|328554838|gb|AEB25330.1| isochorismatase [Bacillus amyloliquefaciens TA208]
 gi|341829114|gb|AEK90365.1| isochorismatase [Bacillus amyloliquefaciens XH7]
Length=306

 Score = 33.9 bits (76),  Expect = 8.7, Method: Compositional matrix adjust.
 Identities = 22/72 (31%), Positives = 38/72 (53%), Gaps = 2/72 (2%)

Query  1    VRDRILAAVCDVLYIDEADLIDGDETDLRDLGLDSVRFVLLMKQLGVNRQSELPSRLAAN  60
             ++R+   +  +L    +D++D  + DL D GLDSVR + L++Q   +        LA N
Sbjct  225  TKERMREQIAAILQESPSDILD--QEDLLDRGLDSVRIMSLVEQWRRDGAEVTFVELAEN  282

Query  61   PSIAGWLRELEA  72
            P++  W R L +
Sbjct  283  PTLEEWWRLLSS  294


>gi|305665878|ref|YP_003862165.1| putative thymidine kinase [Maribacter sp. HTCC2170]
 gi|88710653|gb|EAR02885.1| putative thymidine kinase [Maribacter sp. HTCC2170]
Length=214

 Score = 33.5 bits (75),  Expect = 9.9, Method: Compositional matrix adjust.
 Identities = 29/79 (37%), Positives = 39/79 (50%), Gaps = 9/79 (11%)

Query  4    RILAAVCDVLYIDEADLIDGDE--TDLRDLGLDSVRFVL--LMKQLGVNRQSELPSRLAA  59
            RILA  CDV+ IDEA   D DE  T   DL    VR ++  L      N    +P+ +A 
Sbjct  81   RILADTCDVIGIDEAQFFD-DEIVTVCNDLANRGVRVIVAGLDMDFKGNPFGPMPALMAT  139

Query  60   NPSIAGWLRELEAVCTEFG  78
                A ++ ++ AVCT  G
Sbjct  140  ----AEYVTKVHAVCTRTG  154



Lambda     K      H
   0.323    0.141    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Effective search space used: 129734191374




  Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
    Posted date:  Sep 5, 2011  4:36 AM
  Number of letters in database: 5,219,829,388
  Number of sequences in database:  15,229,318



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40