BLASTP 2.2.25+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 21,062,489 sequences; 7,218,481,314 total letters Query= Rv0397A Rv0397A Conserved protein 476394:476642 forward MW:8504 Length=82 Score E Sequences producing significant alignments: (Bits) Value gi|15839780|ref|NP_334817.1| hypothetical protein MT0407.1 [Myco... 166 2e-39 gi|345462034|ref|YP_004837048.1| hypothetical protein Rv4003 [My... 145 4e-33 gi|307206943|gb|EFN84787.1| Segmentation polarity homeobox prote... 35.4 4.4 >gi|15839780|ref|NP_334817.1| hypothetical protein MT0407.1 [Mycobacterium tuberculosis CDC1551] gi|148821593|ref|YP_001286347.1| hypothetical protein TBFG_10402 [Mycobacterium tuberculosis F11] gi|167970789|ref|ZP_02553066.1| hypothetical protein MtubH3_23220 [Mycobacterium tuberculosis H37Ra] 32 more sequence titlesLength=82 Score = 166 bits (420), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 82/82 (100%), Positives = 82/82 (100%), Gaps = 0/82 (0%) Query 1 MHALRLVGLAILTAIAPIAVLIGSSPAHADTDIGQPCSPEGAKLWGNPGPIYCERTADGQ 60 MHALRLVGLAILTAIAPIAVLIGSSPAHADTDIGQPCSPEGAKLWGNPGPIYCERTADGQ Sbjct 1 MHALRLVGLAILTAIAPIAVLIGSSPAHADTDIGQPCSPEGAKLWGNPGPIYCERTADGQ 60 Query 61 LQWVSIPAWALCVAFCDRPGGP 82 LQWVSIPAWALCVAFCDRPGGP Sbjct 61 LQWVSIPAWALCVAFCDRPGGP 82 >gi|345462034|ref|YP_004837048.1| hypothetical protein Rv4003 [Mycobacterium tuberculosis H37Rv] Length=71 Score = 145 bits (365), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 70/71 (99%), Positives = 71/71 (100%), Gaps = 0/71 (0%) Query 12 LTAIAPIAVLIGSSPAHADTDIGQPCSPEGAKLWGNPGPIYCERTADGQLQWVSIPAWAL 71 +TAIAPIAVLIGSSPAHADTDIGQPCSPEGAKLWGNPGPIYCERTADGQLQWVSIPAWAL Sbjct 1 MTAIAPIAVLIGSSPAHADTDIGQPCSPEGAKLWGNPGPIYCERTADGQLQWVSIPAWAL 60 Query 72 CVAFCDRPGGP 82 CVAFCDRPGGP Sbjct 61 CVAFCDRPGGP 71 >gi|307206943|gb|EFN84787.1| Segmentation polarity homeobox protein engrailed [Harpegnathos saltator] Length=340 Score = 35.4 bits (80), Expect = 4.4, Method: Composition-based stats. Identities = 20/51 (40%), Positives = 26/51 (51%), Gaps = 5/51 (9%) Query 34 GQPCSPEGAKLWGNPG-PIYCERTADGQLQWVSIPAWALCVAFCDRP-GGP 82 G+ + E A+ N G P + T +GQ QW PAW C + DRP GP Sbjct 180 GESLNGESAQSGSNGGTPATQQNTTNGQCQW---PAWVYCTRYSDRPSSGP 227 Lambda K H 0.322 0.140 0.482 Gapped Lambda K H 0.267 0.0410 0.140 Effective search space used: 176962912513 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects Posted date: Oct 14, 2012 4:13 PM Number of letters in database: 7,218,481,314 Number of sequences in database: 21,062,489 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 11 Window for multiple hits: 40