BLASTP 2.2.25+
Reference:
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database
search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
15,229,318 sequences; 5,219,829,388 total letters
Query= Rv0454
Length=116
Score E
Sequences producing significant alignments: (Bits) Value
gi|15607595|ref|NP_214968.1| hypothetical protein Rv0454 [Mycoba... 235 2e-60
gi|340625479|ref|YP_004743931.1| hypothetical protein MCAN_04521... 228 2e-58
gi|699244|gb|AAA63007.1| u1740u [Mycobacterium leprae] 45.1 0.003
gi|307106116|gb|EFN54363.1| hypothetical protein CHLNCDRAFT_5318... 34.3 5.6
gi|116623836|ref|YP_825992.1| hypothetical protein Acid_4748 [Ca... 33.9 7.4
>gi|15607595|ref|NP_214968.1| hypothetical protein Rv0454 [Mycobacterium tuberculosis H37Rv]
gi|31791632|ref|NP_854125.1| hypothetical protein Mb0462 [Mycobacterium bovis AF2122/97]
gi|121636368|ref|YP_976591.1| hypothetical protein BCG_0493 [Mycobacterium bovis BCG str. Pasteur
1173P2]
21 more sequence titles
Length=116
Score = 235 bits (599), Expect = 2e-60, Method: Compositional matrix adjust.
Identities = 115/116 (99%), Positives = 116/116 (100%), Gaps = 0/116 (0%)
Query 1 LKQDFGLDVPQAGNAQNFDGVPEWVQVGVVTFVYRMQMHHVTRPVGAPGSGLAGDSTPVQ 60
+KQDFGLDVPQAGNAQNFDGVPEWVQVGVVTFVYRMQMHHVTRPVGAPGSGLAGDSTPVQ
Sbjct 1 MKQDFGLDVPQAGNAQNFDGVPEWVQVGVVTFVYRMQMHHVTRPVGAPGSGLAGDSTPVQ 60
Query 61 GRQRVWDLVAGRLTHAPRSSVQAMRPTMFTSAPQRHGIPARGRWWLGYQERSRAWP 116
GRQRVWDLVAGRLTHAPRSSVQAMRPTMFTSAPQRHGIPARGRWWLGYQERSRAWP
Sbjct 61 GRQRVWDLVAGRLTHAPRSSVQAMRPTMFTSAPQRHGIPARGRWWLGYQERSRAWP 116
>gi|340625479|ref|YP_004743931.1| hypothetical protein MCAN_04521 [Mycobacterium canettii CIPT
140010059]
gi|340003669|emb|CCC42792.1| conserved hypothetical protein [Mycobacterium canettii CIPT 140010059]
Length=115
Score = 228 bits (581), Expect = 2e-58, Method: Compositional matrix adjust.
Identities = 114/116 (99%), Positives = 115/116 (99%), Gaps = 1/116 (0%)
Query 1 LKQDFGLDVPQAGNAQNFDGVPEWVQVGVVTFVYRMQMHHVTRPVGAPGSGLAGDSTPVQ 60
+KQDFGLDVPQAGNAQNFDGVPEWVQVGVVTFVYRMQMHHVTRPVGAPGSGLAGDSTPVQ
Sbjct 1 MKQDFGLDVPQAGNAQNFDGVPEWVQVGVVTFVYRMQMHHVTRPVGAPGSGLAGDSTPVQ 60
Query 61 GRQRVWDLVAGRLTHAPRSSVQAMRPTMFTSAPQRHGIPARGRWWLGYQERSRAWP 116
GRQRVWDLVAGRLTHAPRSSVQAMRP MFTSAPQRHGIPARGRWWLGYQERSRAWP
Sbjct 61 GRQRVWDLVAGRLTHAPRSSVQAMRP-MFTSAPQRHGIPARGRWWLGYQERSRAWP 115
>gi|699244|gb|AAA63007.1| u1740u [Mycobacterium leprae]
Length=115
Score = 45.1 bits (105), Expect = 0.003, Method: Compositional matrix adjust.
Identities = 28/89 (32%), Positives = 47/89 (53%), Gaps = 5/89 (5%)
Query 8 DVPQAGN---AQNFDGVPEWVQVGVVTFVYRMQMHHVTRPVGAPGSGLAGDSTPVQGRQR 64
D P+A + AQ+ D V VQ+ + F+Y + +H + +GA LA + P++G +R
Sbjct 25 DEPEAAHTAEAQDVDDVSAQVQIRIAAFIYGVPVHDMGSRIGAARFRLARELMPIEGSKR 84
Query 65 VWDLVAGRLTHAPRSSVQAMRPTMFTSAP 93
V DLV L H +S+ + P+ + P
Sbjct 85 VRDLVTRYLAHV--TSIVSPAPSCIGAEP 111
>gi|307106116|gb|EFN54363.1| hypothetical protein CHLNCDRAFT_53186 [Chlorella variabilis]
Length=3414
Score = 34.3 bits (77), Expect = 5.6, Method: Compositional matrix adjust.
Identities = 21/59 (36%), Positives = 29/59 (50%), Gaps = 2/59 (3%)
Query 32 FVYRMQMHHVTRPVGAPGSGLAGDSTPVQGRQRVWDLVAGRLTHAPRSSVQAMRPTMFT 90
FV RM ++ R PGS L GD +PV + +A + P+S + MRP M T
Sbjct 685 FVPRMLRRYILRSHPPPGSSLVGDGSPVH--DSIGKALASKAAAPPQSLSRVMRPCMDT 741
>gi|116623836|ref|YP_825992.1| hypothetical protein Acid_4748 [Candidatus Solibacter usitatus
Ellin6076]
gi|116226998|gb|ABJ85707.1| hypothetical protein Acid_4748 [Candidatus Solibacter usitatus
Ellin6076]
Length=492
Score = 33.9 bits (76), Expect = 7.4, Method: Compositional matrix adjust.
Identities = 19/51 (38%), Positives = 28/51 (55%), Gaps = 0/51 (0%)
Query 52 LAGDSTPVQGRQRVWDLVAGRLTHAPRSSVQAMRPTMFTSAPQRHGIPARG 102
LAGD P + +D++ R+ PRS++QAMR T + P G+ RG
Sbjct 379 LAGDFEPPAEHRSRYDILPVRVGTDPRSAMQAMRGTGYYKVPSLKGVWYRG 429
Lambda K H
0.321 0.136 0.453
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 130541267802
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Sep 5, 2011 4:36 AM
Number of letters in database: 5,219,829,388
Number of sequences in database: 15,229,318
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40