BLASTP 2.2.25+
Reference:
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database
search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
15,229,318 sequences; 5,219,829,388 total letters
Query= Rv0492A
Length=109
Score E
Sequences producing significant alignments: (Bits) Value
gi|31791673|ref|NP_854166.1| hypothetical protein Mb0503c [Mycob... 218 2e-55
gi|296168102|ref|ZP_06850168.1| conserved hypothetical protein [... 186 8e-46
gi|118619655|ref|YP_907987.1| hypothetical protein MUL_4562 [Myc... 184 4e-45
gi|240171895|ref|ZP_04750554.1| hypothetical protein MkanA1_2145... 177 6e-43
gi|284042663|ref|YP_003393003.1| hypothetical protein Cwoe_1199 ... 69.7 2e-10
gi|170028174|ref|XP_001841971.1| glycerol kinase [Culex quinquef... 36.2 1.7
>gi|31791673|ref|NP_854166.1| hypothetical protein Mb0503c [Mycobacterium bovis AF2122/97]
gi|57116741|ref|YP_177623.1| hypothetical protein Rv0492A [Mycobacterium tuberculosis H37Rv]
gi|121636409|ref|YP_976632.1| hypothetical protein BCG_0534c [Mycobacterium bovis BCG str.
Pasteur 1173P2]
72 more sequence titles
Length=109
Score = 218 bits (556), Expect = 2e-55, Method: Compositional matrix adjust.
Identities = 109/109 (100%), Positives = 109/109 (100%), Gaps = 0/109 (0%)
Query 1 MSFLLDPPLLFVCGVLIERRLPVDRRDAAEAAALGVFFGASFGLYHNVPGLGMLWRPFRA 60
MSFLLDPPLLFVCGVLIERRLPVDRRDAAEAAALGVFFGASFGLYHNVPGLGMLWRPFRA
Sbjct 1 MSFLLDPPLLFVCGVLIERRLPVDRRDAAEAAALGVFFGASFGLYHNVPGLGMLWRPFRA 60
Query 61 QNGRDFMWNSGVFSVDVARAEWPLHAMAAAIFATYPFFIKLGRRLGRRI 109
QNGRDFMWNSGVFSVDVARAEWPLHAMAAAIFATYPFFIKLGRRLGRRI
Sbjct 61 QNGRDFMWNSGVFSVDVARAEWPLHAMAAAIFATYPFFIKLGRRLGRRI 109
>gi|296168102|ref|ZP_06850168.1| conserved hypothetical protein [Mycobacterium parascrofulaceum
ATCC BAA-614]
gi|295896909|gb|EFG76537.1| conserved hypothetical protein [Mycobacterium parascrofulaceum
ATCC BAA-614]
Length=111
Score = 186 bits (473), Expect = 8e-46, Method: Compositional matrix adjust.
Identities = 91/109 (84%), Positives = 97/109 (89%), Gaps = 0/109 (0%)
Query 1 MSFLLDPPLLFVCGVLIERRLPVDRRDAAEAAALGVFFGASFGLYHNVPGLGMLWRPFRA 60
MSFLLDPPLLF GVLIERRLP DRRD AEAA LGVFFG SFGLY+NVPGLG+LWRPFRA
Sbjct 3 MSFLLDPPLLFASGVLIERRLPADRRDVAEAATLGVFFGGSFGLYNNVPGLGVLWRPFRA 62
Query 61 QNGRDFMWNSGVFSVDVARAEWPLHAMAAAIFATYPFFIKLGRRLGRRI 109
+NGRDFMWNSGVFSV+ A A+WPLHA A AIFATYPFFIK+GRRL R I
Sbjct 63 RNGRDFMWNSGVFSVNTAEADWPLHAAAGAIFATYPFFIKMGRRLARLI 111
>gi|118619655|ref|YP_907987.1| hypothetical protein MUL_4562 [Mycobacterium ulcerans Agy99]
gi|183980842|ref|YP_001849133.1| hypothetical protein MMAR_0818 [Mycobacterium marinum M]
gi|118571765|gb|ABL06516.1| conserved hypothetical membrane protein [Mycobacterium ulcerans
Agy99]
gi|183174168|gb|ACC39278.1| conserved hypothetical membrane protein [Mycobacterium marinum
M]
Length=113
Score = 184 bits (467), Expect = 4e-45, Method: Compositional matrix adjust.
Identities = 89/109 (82%), Positives = 96/109 (89%), Gaps = 0/109 (0%)
Query 1 MSFLLDPPLLFVCGVLIERRLPVDRRDAAEAAALGVFFGASFGLYHNVPGLGMLWRPFRA 60
MSFLLDPPLLF CGVLIER L DRRD AEAAALGVFF SFGLY+NVPGLG+LWRPFRA
Sbjct 1 MSFLLDPPLLFACGVLIERHLDEDRRDLAEAAALGVFFAGSFGLYNNVPGLGLLWRPFRA 60
Query 61 QNGRDFMWNSGVFSVDVARAEWPLHAMAAAIFATYPFFIKLGRRLGRRI 109
+NGRDFMWNSGVF+VD +A+WPLHA A IFATYPFFIKLGRRLG R+
Sbjct 61 RNGRDFMWNSGVFNVDTDQAQWPLHAAAGGIFATYPFFIKLGRRLGSRL 109
>gi|240171895|ref|ZP_04750554.1| hypothetical protein MkanA1_21453 [Mycobacterium kansasii ATCC
12478]
Length=109
Score = 177 bits (448), Expect = 6e-43, Method: Compositional matrix adjust.
Identities = 86/108 (80%), Positives = 89/108 (83%), Gaps = 0/108 (0%)
Query 1 MSFLLDPPLLFVCGVLIERRLPVDRRDAAEAAALGVFFGASFGLYHNVPGLGMLWRPFRA 60
MSFLLDPPLLF G LIERR+P DRRD EAA LGVFF SFGLY+NVPGLG LWRPFR+
Sbjct 1 MSFLLDPPLLFASGALIERRVPEDRRDLIEAAVLGVFFAGSFGLYNNVPGLGFLWRPFRS 60
Query 61 QNGRDFMWNSGVFSVDVARAEWPLHAMAAAIFATYPFFIKLGRRLGRR 108
QNGRDFMWNSGVF VD A A WPLHA A IF TYPFFIKLGRR GRR
Sbjct 61 QNGRDFMWNSGVFDVDTAEAGWPLHAAAGGIFGTYPFFIKLGRRFGRR 108
>gi|284042663|ref|YP_003393003.1| hypothetical protein Cwoe_1199 [Conexibacter woesei DSM 14684]
gi|283946884|gb|ADB49628.1| hypothetical protein Cwoe_1199 [Conexibacter woesei DSM 14684]
Length=117
Score = 69.7 bits (169), Expect = 2e-10, Method: Compositional matrix adjust.
Identities = 48/115 (42%), Positives = 61/115 (54%), Gaps = 8/115 (6%)
Query 1 MSFLLDPPLLFVCGVLIER--------RLPVDRRDAAEAAALGVFFGASFGLYHNVPGLG 52
MSFL+DP LL+ G R R A A + VF+G S GLY N
Sbjct 1 MSFLIDPALLYAGGRTYRRLAADADAAAPDPARDAAVAVAFMTVFWGVSVGLYLNHGWTK 60
Query 53 MLWRPFRAQNGRDFMWNSGVFSVDVARAEWPLHAMAAAIFATYPFFIKLGRRLGR 107
+W RA++GRD+M NSGV +D ARA H ++A IFATYP ++ LG R R
Sbjct 61 PIWWICRARSGRDWMLNSGVLRIDDARAGRRTHLVSALIFATYPLWLWLGMRQRR 115
>gi|170028174|ref|XP_001841971.1| glycerol kinase [Culex quinquefasciatus]
gi|167871796|gb|EDS35179.1| glycerol kinase [Culex quinquefasciatus]
Length=508
Score = 36.2 bits (82), Expect = 1.7, Method: Composition-based stats.
Identities = 18/42 (43%), Positives = 26/42 (62%), Gaps = 3/42 (7%)
Query 11 FVCGVLIERR-LPVDRRDAAEAAALGVFF--GASFGLYHNVP 49
F+C +L + LPV+R D A+A+ALG F G + G + N P
Sbjct 429 FICQMLADTAGLPVERSDTADASALGAVFVAGINLGFFRNKP 470
Lambda K H
0.333 0.146 0.484
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 129509500864
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Sep 5, 2011 4:36 AM
Number of letters in database: 5,219,829,388
Number of sequences in database: 15,229,318
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40