BLASTP 2.2.25+
Reference:
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database
search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
15,229,318 sequences; 5,219,829,388 total letters
Query= Rv0514
Length=99
Score E
Sequences producing significant alignments: (Bits) Value
gi|15607655|ref|NP_215028.1| transmembrane protein [Mycobacteriu... 194 5e-48
gi|121636432|ref|YP_976655.1| putative transmembrane protein [My... 192 2e-47
gi|118463663|ref|YP_883764.1| transmembrane protein [Mycobacteri... 81.6 4e-14
gi|118619691|ref|YP_908023.1| transmembrane protein [Mycobacteri... 77.8 5e-13
gi|240173365|ref|ZP_04752023.1| hypothetical protein MkanA1_2889... 72.0 3e-11
gi|254821054|ref|ZP_05226055.1| putative transmembrane protein [... 72.0 3e-11
gi|342858981|ref|ZP_08715635.1| transmembrane protein [Mycobacte... 71.6 3e-11
gi|296168173|ref|ZP_06850194.1| transmembrane protein [Mycobacte... 55.1 4e-06
gi|333989151|ref|YP_004521765.1| transmembrane protein [Mycobact... 53.1 1e-05
gi|120401866|ref|YP_951695.1| putative transmembrane protein [My... 52.8 2e-05
gi|145220667|ref|YP_001131345.1| hypothetical protein Mflv_0061 ... 47.0 0.001
gi|118472625|ref|YP_885363.1| transmembrane protein [Mycobacteri... 43.5 0.011
gi|84993817|gb|ABC68329.1| OrfB [Mycobacterium fortuitum] 36.2 1.7
gi|108797663|ref|YP_637860.1| hypothetical protein Mmcs_0683 [My... 34.3 6.8
>gi|15607655|ref|NP_215028.1| transmembrane protein [Mycobacterium tuberculosis H37Rv]
gi|15839907|ref|NP_334944.1| hypothetical protein MT0535 [Mycobacterium tuberculosis CDC1551]
gi|31791696|ref|NP_854189.1| transmembrane protein [Mycobacterium bovis AF2122/97]
73 more sequence titles
Length=99
Score = 194 bits (492), Expect = 5e-48, Method: Compositional matrix adjust.
Identities = 98/99 (99%), Positives = 99/99 (100%), Gaps = 0/99 (0%)
Query 1 VIARYRAGAELFLACAALAGSAASWSRTRSTVAVAPVIDGQPVTLSVVYHPQPLVLTLLL 60
+IARYRAGAELFLACAALAGSAASWSRTRSTVAVAPVIDGQPVTLSVVYHPQPLVLTLLL
Sbjct 1 MIARYRAGAELFLACAALAGSAASWSRTRSTVAVAPVIDGQPVTLSVVYHPQPLVLTLLL 60
Query 61 ATIAGVLSVVGTARLRRARAGLNAHPDGLNQRPPGGWCH 99
ATIAGVLSVVGTARLRRARAGLNAHPDGLNQRPPGGWCH
Sbjct 61 ATIAGVLSVVGTARLRRARAGLNAHPDGLNQRPPGGWCH 99
>gi|121636432|ref|YP_976655.1| putative transmembrane protein [Mycobacterium bovis BCG str.
Pasteur 1173P2]
gi|224988904|ref|YP_002643591.1| putative transmembrane protein [Mycobacterium bovis BCG str.
Tokyo 172]
gi|121492079|emb|CAL70542.1| Possible transmembrane protein [Mycobacterium bovis BCG str.
Pasteur 1173P2]
gi|224772017|dbj|BAH24823.1| putative transmembrane protein [Mycobacterium bovis BCG str.
Tokyo 172]
gi|341600448|emb|CCC63118.1| possible transmembrane protein [Mycobacterium bovis BCG str.
Moreau RDJ]
Length=99
Score = 192 bits (488), Expect = 2e-47, Method: Compositional matrix adjust.
Identities = 97/99 (98%), Positives = 98/99 (99%), Gaps = 0/99 (0%)
Query 1 VIARYRAGAELFLACAALAGSAASWSRTRSTVAVAPVIDGQPVTLSVVYHPQPLVLTLLL 60
+IARYRAGAELFLACAALAGSA SWSRTRSTVAVAPVIDGQPVTLSVVYHPQPLVLTLLL
Sbjct 1 MIARYRAGAELFLACAALAGSAGSWSRTRSTVAVAPVIDGQPVTLSVVYHPQPLVLTLLL 60
Query 61 ATIAGVLSVVGTARLRRARAGLNAHPDGLNQRPPGGWCH 99
ATIAGVLSVVGTARLRRARAGLNAHPDGLNQRPPGGWCH
Sbjct 61 ATIAGVLSVVGTARLRRARAGLNAHPDGLNQRPPGGWCH 99
>gi|118463663|ref|YP_883764.1| transmembrane protein [Mycobacterium avium 104]
gi|254777072|ref|ZP_05218588.1| putative transmembrane protein [Mycobacterium avium subsp. avium
ATCC 25291]
gi|118164950|gb|ABK65847.1| putative transmembrane protein [Mycobacterium avium 104]
gi|336460519|gb|EGO39414.1| hypothetical protein MAPs_40360 [Mycobacterium avium subsp. paratuberculosis
S397]
Length=86
Score = 81.6 bits (200), Expect = 4e-14, Method: Compositional matrix adjust.
Identities = 53/80 (67%), Positives = 61/80 (77%), Gaps = 0/80 (0%)
Query 1 VIARYRAGAELFLACAALAGSAASWSRTRSTVAVAPVIDGQPVTLSVVYHPQPLVLTLLL 60
+IA RA EL LA AALAGSA SW R+ +VAVAP+ DGQP T SVVY PQ L+LTL+L
Sbjct 1 MIAGSRAVVELVLAVAALAGSAVSWVRSHHSVAVAPIADGQPFTTSVVYDPQLLLLTLVL 60
Query 61 ATIAGVLSVVGTARLRRARA 80
T AGVL+VVG RLRRAR+
Sbjct 61 LTSAGVLAVVGITRLRRARS 80
>gi|118619691|ref|YP_908023.1| transmembrane protein [Mycobacterium ulcerans Agy99]
gi|183980872|ref|YP_001849163.1| transmembrane protein [Mycobacterium marinum M]
gi|118571801|gb|ABL06552.1| transmembrane protein [Mycobacterium ulcerans Agy99]
gi|183174198|gb|ACC39308.1| transmembrane protein [Mycobacterium marinum M]
Length=94
Score = 77.8 bits (190), Expect = 5e-13, Method: Compositional matrix adjust.
Identities = 55/85 (65%), Positives = 62/85 (73%), Gaps = 0/85 (0%)
Query 4 RYRAGAELFLACAALAGSAASWSRTRSTVAVAPVIDGQPVTLSVVYHPQPLVLTLLLATI 63
R RA EL LA A+ +AASW +T STVAVAPV GQPVT SV+YHPQ LVLTL LAT+
Sbjct 4 RNRAVIELALALVAVWATAASWLQTTSTVAVAPVTAGQPVTTSVIYHPQQLVLTLALATL 63
Query 64 AGVLSVVGTARLRRARAGLNAHPDG 88
AGVL+VVG RLRRAR +A D
Sbjct 64 AGVLAVVGVCRLRRARQSPSAPADN 88
>gi|240173365|ref|ZP_04752023.1| hypothetical protein MkanA1_28891 [Mycobacterium kansasii ATCC
12478]
Length=93
Score = 72.0 bits (175), Expect = 3e-11, Method: Compositional matrix adjust.
Identities = 58/87 (67%), Positives = 67/87 (78%), Gaps = 1/87 (1%)
Query 1 VIARYRAGAELFLACAALAGSAASWSRTRSTVAVAPVIDGQPVTLSVVYHPQPLVLTLLL 60
+ AR+RA AEL +A AA+ +AASW TRSTV V PV DGQPVT+SVVYHPQ LVLTLLL
Sbjct 1 MTARHRAMAELTVALAAVVATAASWLHTRSTVVVGPVADGQPVTVSVVYHPQLLVLTLLL 60
Query 61 ATIAGVLSVVGTARLRR-ARAGLNAHP 86
AT+AG+ +VVG ARLRR AR A P
Sbjct 61 ATVAGIFAVVGAARLRRSARPSAGAEP 87
>gi|254821054|ref|ZP_05226055.1| putative transmembrane protein [Mycobacterium intracellulare
ATCC 13950]
Length=86
Score = 72.0 bits (175), Expect = 3e-11, Method: Compositional matrix adjust.
Identities = 54/81 (67%), Positives = 63/81 (78%), Gaps = 0/81 (0%)
Query 1 VIARYRAGAELFLACAALAGSAASWSRTRSTVAVAPVIDGQPVTLSVVYHPQPLVLTLLL 60
+IA YRA EL +A AALAGS SW+R+ TVAVAP+ +GQP T SVVY PQ L+LTL+L
Sbjct 1 MIASYRALVELAVAVAALAGSGYSWTRSHHTVAVAPIAEGQPFTTSVVYDPQLLLLTLVL 60
Query 61 ATIAGVLSVVGTARLRRARAG 81
T AGVL VVG ARLRRAR+G
Sbjct 61 LTSAGVLVVVGAARLRRARSG 81
>gi|342858981|ref|ZP_08715635.1| transmembrane protein [Mycobacterium colombiense CECT 3035]
gi|342133222|gb|EGT86425.1| transmembrane protein [Mycobacterium colombiense CECT 3035]
Length=86
Score = 71.6 bits (174), Expect = 3e-11, Method: Compositional matrix adjust.
Identities = 46/78 (59%), Positives = 55/78 (71%), Gaps = 0/78 (0%)
Query 3 ARYRAGAELFLACAALAGSAASWSRTRSTVAVAPVIDGQPVTLSVVYHPQPLVLTLLLAT 62
ARYRA E LAC AL S +W + TV VAP+ DGQP T S+ Y PQ L+LTL+L T
Sbjct 3 ARYRATLEFGLACVALVCSGLTWWHSHHTVTVAPIADGQPSTTSLSYDPQLLLLTLVLLT 62
Query 63 IAGVLSVVGTARLRRARA 80
AGVL+V+GTARL RAR+
Sbjct 63 SAGVLAVLGTARLLRARS 80
>gi|296168173|ref|ZP_06850194.1| transmembrane protein [Mycobacterium parascrofulaceum ATCC BAA-614]
gi|295896851|gb|EFG76480.1| transmembrane protein [Mycobacterium parascrofulaceum ATCC BAA-614]
Length=87
Score = 55.1 bits (131), Expect = 4e-06, Method: Compositional matrix adjust.
Identities = 35/50 (70%), Positives = 39/50 (78%), Gaps = 0/50 (0%)
Query 24 SWSRTRSTVAVAPVIDGQPVTLSVVYHPQPLVLTLLLATIAGVLSVVGTA 73
SWSR+ TVAVAP+ DGQP T S VY PQ L+LTLLL AGVL+VVGTA
Sbjct 27 SWSRSHHTVAVAPIADGQPFTTSTVYDPQLLLLTLLLLIGAGVLAVVGTA 76
>gi|333989151|ref|YP_004521765.1| transmembrane protein [Mycobacterium sp. JDM601]
gi|333485119|gb|AEF34511.1| transmembrane protein [Mycobacterium sp. JDM601]
Length=92
Score = 53.1 bits (126), Expect = 1e-05, Method: Compositional matrix adjust.
Identities = 42/67 (63%), Positives = 47/67 (71%), Gaps = 0/67 (0%)
Query 6 RAGAELFLACAALAGSAASWSRTRSTVAVAPVIDGQPVTLSVVYHPQPLVLTLLLATIAG 65
R AEL LA AA G A SW R V VAPVIDG+P T SV YHP+ L+L LLLAT+AG
Sbjct 8 RGCAELTLAGAAALGCAVSWMHVRYLVLVAPVIDGEPATTSVTYHPRMLLLALLLATVAG 67
Query 66 VLSVVGT 72
VL+V GT
Sbjct 68 VLAVTGT 74
>gi|120401866|ref|YP_951695.1| putative transmembrane protein [Mycobacterium vanbaalenii PYR-1]
gi|119954684|gb|ABM11689.1| putative transmembrane protein [Mycobacterium vanbaalenii PYR-1]
Length=83
Score = 52.8 bits (125), Expect = 2e-05, Method: Compositional matrix adjust.
Identities = 40/69 (58%), Positives = 48/69 (70%), Gaps = 0/69 (0%)
Query 9 AELFLACAALAGSAASWSRTRSTVAVAPVIDGQPVTLSVVYHPQPLVLTLLLATIAGVLS 68
A+L A A GS SW +TV VAPV+DG+P T SVVY+ L L+LLLAT AGVL+
Sbjct 5 AQLGAALLAGVGSVLSWLAASATVTVAPVLDGEPETTSVVYNAPLLTLSLLLATAAGVLT 64
Query 69 VVGTARLRR 77
V+G ARLRR
Sbjct 65 VLGVARLRR 73
>gi|145220667|ref|YP_001131345.1| hypothetical protein Mflv_0061 [Mycobacterium gilvum PYR-GCK]
gi|315442383|ref|YP_004075262.1| hypothetical protein Mspyr1_07270 [Mycobacterium sp. Spyr1]
gi|145213153|gb|ABP42557.1| putative transmembrane protein [Mycobacterium gilvum PYR-GCK]
gi|315260686|gb|ADT97427.1| hypothetical protein Mspyr1_07270 [Mycobacterium sp. Spyr1]
Length=84
Score = 47.0 bits (110), Expect = 0.001, Method: Compositional matrix adjust.
Identities = 39/79 (50%), Positives = 50/79 (64%), Gaps = 0/79 (0%)
Query 2 IARYRAGAELFLACAALAGSAASWSRTRSTVAVAPVIDGQPVTLSVVYHPQPLVLTLLLA 61
++R RA AE+ L A G A+W + V VAPV+ G+P T S Y+ L L+LLLA
Sbjct 1 MSRRRALAEVLLGALAAVGCVAAWLGAATPVTVAPVLPGEPSTSSYTYYAPMLTLSLLLA 60
Query 62 TIAGVLSVVGTARLRRARA 80
T+AGVL V+G ARLRR A
Sbjct 61 TLAGVLVVLGIARLRRTSA 79
>gi|118472625|ref|YP_885363.1| transmembrane protein [Mycobacterium smegmatis str. MC2 155]
gi|118173912|gb|ABK74808.1| putative transmembrane protein [Mycobacterium smegmatis str.
MC2 155]
Length=76
Score = 43.5 bits (101), Expect = 0.011, Method: Compositional matrix adjust.
Identities = 33/55 (60%), Positives = 40/55 (73%), Gaps = 1/55 (1%)
Query 24 SWSRTRSTVAVAPVIDGQPVTLSVVYHPQPLVLTLLLATIAGVLSVVGTAR-LRR 77
SW +R+TV VAPV+ G+PVT SV Y L L LLLAT+AGVL+V+G A LRR
Sbjct 22 SWVASRATVEVAPVLPGEPVTYSVTYSAPMLTLALLLATVAGVLAVLGVANALRR 76
>gi|84993817|gb|ABC68329.1| OrfB [Mycobacterium fortuitum]
Length=75
Score = 36.2 bits (82), Expect = 1.7, Method: Compositional matrix adjust.
Identities = 35/74 (48%), Positives = 47/74 (64%), Gaps = 0/74 (0%)
Query 4 RYRAGAELFLACAALAGSAASWSRTRSTVAVAPVIDGQPVTLSVVYHPQPLVLTLLLATI 63
+ RA EL +A A G SW +T+ VAPV++G+P T ++ Y LVL L LA +
Sbjct 2 KRRAVVELAVAAIAAVGCVLSWIAAATTIEVAPVLEGEPATTAISYSSPLLVLALALAGL 61
Query 64 AGVLSVVGTARLRR 77
AGVL+V+G ARLRR
Sbjct 62 AGVLAVLGIARLRR 75
>gi|108797663|ref|YP_637860.1| hypothetical protein Mmcs_0683 [Mycobacterium sp. MCS]
gi|119866750|ref|YP_936702.1| hypothetical protein Mkms_0696 [Mycobacterium sp. KMS]
gi|126433287|ref|YP_001068978.1| hypothetical protein Mjls_0676 [Mycobacterium sp. JLS]
gi|108768082|gb|ABG06804.1| putative transmembrane protein [Mycobacterium sp. MCS]
gi|119692839|gb|ABL89912.1| putative transmembrane protein [Mycobacterium sp. KMS]
gi|126233087|gb|ABN96487.1| hypothetical protein Mjls_0676 [Mycobacterium sp. JLS]
Length=78
Score = 34.3 bits (77), Expect = 6.8, Method: Compositional matrix adjust.
Identities = 40/75 (54%), Positives = 46/75 (62%), Gaps = 0/75 (0%)
Query 3 ARYRAGAELFLACAALAGSAASWSRTRSTVAVAPVIDGQPVTLSVVYHPQPLVLTLLLAT 62
AR RA EL LA AA G SW+ APV+ G+P T+ VY LVL LLLAT
Sbjct 3 ARSRALFELALAAAAAVGCVLSWAAAGRPATAAPVMPGEPETVVTVYSAPLLVLALLLAT 62
Query 63 IAGVLSVVGTARLRR 77
+AGVL V+G ARLRR
Sbjct 63 VAGVLLVLGVARLRR 77
Lambda K H
0.323 0.135 0.419
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 129711308684
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Sep 5, 2011 4:36 AM
Number of letters in database: 5,219,829,388
Number of sequences in database: 15,229,318
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40