BLASTP 2.2.25+
Reference:
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database
search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
15,229,318 sequences; 5,219,829,388 total letters
Query= Rv0612
Length=201
Score E
Sequences producing significant alignments: (Bits) Value
gi|15607752|ref|NP_215126.1| hypothetical protein Rv0612 [Mycoba... 392 1e-107
gi|308231600|ref|ZP_07413056.2| hypothetical protein TMAG_02492 ... 356 1e-96
gi|307083110|ref|ZP_07492223.1| hypothetical protein TMLG_03359 ... 342 1e-92
gi|333989262|ref|YP_004521876.1| hypothetical protein JDM601_062... 171 5e-41
gi|260907600|ref|ZP_05915922.1| hypothetical protein BlinB_19847... 119 2e-25
gi|31791792|ref|NP_854285.1| hypothetical protein Mb0626 [Mycoba... 50.1 2e-04
gi|15840010|ref|NP_335047.1| hypothetical protein MT0638.1 [Myco... 49.3 4e-04
gi|336115309|ref|YP_004570076.1| hypothetical protein BCO26_2632... 48.1 7e-04
gi|255280369|ref|ZP_05344924.1| hypothetical protein BRYFOR_0570... 47.0 0.001
gi|289441999|ref|ZP_06431743.1| conserved hypothetical protein [... 47.0 0.002
gi|229542672|ref|ZP_04431732.1| hypothetical protein BcoaDRAFT_5... 45.1 0.007
gi|337769103|emb|CCB77816.1| putative FMN-dependent monooxygenas... 41.2 0.095
gi|124006831|ref|ZP_01691661.1| hypothetical protein M23134_0653... 38.5 0.57
gi|317038352|ref|XP_001402108.2| pH-response regulator protein p... 34.7 7.1
gi|134074717|emb|CAK38902.1| unnamed protein product [Aspergillu... 34.7 7.4
gi|310791717|gb|EFQ27244.1| NACHT and TPR domain-containing prot... 34.7 8.5
>gi|15607752|ref|NP_215126.1| hypothetical protein Rv0612 [Mycobacterium tuberculosis H37Rv]
gi|15840014|ref|NP_335051.1| hypothetical protein MT0642 [Mycobacterium tuberculosis CDC1551]
gi|31791795|ref|NP_854288.1| hypothetical protein Mb0629 [Mycobacterium bovis AF2122/97]
60 more sequence titles
Length=201
Score = 392 bits (1008), Expect = 1e-107, Method: Compositional matrix adjust.
Identities = 200/201 (99%), Positives = 201/201 (100%), Gaps = 0/201 (0%)
Query 1 VLGPIRQPRLTVRPGRLPGMIAGVAAKRMNREQFFRAASGLDEDRLRKALWNLYWRGTAN 60
+LGPIRQPRLTVRPGRLPGMIAGVAAKRMNREQFFRAASGLDEDRLRKALWNLYWRGTAN
Sbjct 1 MLGPIRQPRLTVRPGRLPGMIAGVAAKRMNREQFFRAASGLDEDRLRKALWNLYWRGTAN 60
Query 61 MRERIEAELASAGRARPARKIKPPADPDIVGWEVDEFVSLARSGAYLGGDRRVSPRERSR 120
MRERIEAELASAGRARPARKIKPPADPDIVGWEVDEFVSLARSGAYLGGDRRVSPRERSR
Sbjct 61 MRERIEAELASAGRARPARKIKPPADPDIVGWEVDEFVSLARSGAYLGGDRRVSPRERSR 120
Query 121 WRFTFKRLAAEAQDALRAEDAEPAASALEQLIDLAREADGYDYFRSDDPVAAAGFVVSDV 180
WRFTFKRLAAEAQDALRAEDAEPAASALEQLIDLAREADGYDYFRSDDPVAAAGFVVSDV
Sbjct 121 WRFTFKRLAAEAQDALRAEDAEPAASALEQLIDLAREADGYDYFRSDDPVAAAGFVVSDV 180
Query 181 AAAGHPHFREFAAEIGAAIPP 201
AAAGHPHFREFAAEIGAAIPP
Sbjct 181 AAAGHPHFREFAAEIGAAIPP 201
>gi|308231600|ref|ZP_07413056.2| hypothetical protein TMAG_02492 [Mycobacterium tuberculosis SUMu001]
gi|308369992|ref|ZP_07419888.2| hypothetical protein TMBG_03473 [Mycobacterium tuberculosis SUMu002]
gi|308370461|ref|ZP_07421579.2| hypothetical protein TMCG_03818 [Mycobacterium tuberculosis SUMu003]
15 more sequence titles
Length=182
Score = 356 bits (913), Expect = 1e-96, Method: Compositional matrix adjust.
Identities = 182/182 (100%), Positives = 182/182 (100%), Gaps = 0/182 (0%)
Query 20 MIAGVAAKRMNREQFFRAASGLDEDRLRKALWNLYWRGTANMRERIEAELASAGRARPAR 79
MIAGVAAKRMNREQFFRAASGLDEDRLRKALWNLYWRGTANMRERIEAELASAGRARPAR
Sbjct 1 MIAGVAAKRMNREQFFRAASGLDEDRLRKALWNLYWRGTANMRERIEAELASAGRARPAR 60
Query 80 KIKPPADPDIVGWEVDEFVSLARSGAYLGGDRRVSPRERSRWRFTFKRLAAEAQDALRAE 139
KIKPPADPDIVGWEVDEFVSLARSGAYLGGDRRVSPRERSRWRFTFKRLAAEAQDALRAE
Sbjct 61 KIKPPADPDIVGWEVDEFVSLARSGAYLGGDRRVSPRERSRWRFTFKRLAAEAQDALRAE 120
Query 140 DAEPAASALEQLIDLAREADGYDYFRSDDPVAAAGFVVSDVAAAGHPHFREFAAEIGAAI 199
DAEPAASALEQLIDLAREADGYDYFRSDDPVAAAGFVVSDVAAAGHPHFREFAAEIGAAI
Sbjct 121 DAEPAASALEQLIDLAREADGYDYFRSDDPVAAAGFVVSDVAAAGHPHFREFAAEIGAAI 180
Query 200 PP 201
PP
Sbjct 181 PP 182
>gi|307083110|ref|ZP_07492223.1| hypothetical protein TMLG_03359 [Mycobacterium tuberculosis SUMu012]
gi|308367186|gb|EFP56037.1| hypothetical protein TMLG_03359 [Mycobacterium tuberculosis SUMu012]
Length=189
Score = 342 bits (878), Expect = 1e-92, Method: Compositional matrix adjust.
Identities = 176/188 (94%), Positives = 177/188 (95%), Gaps = 0/188 (0%)
Query 1 VLGPIRQPRLTVRPGRLPGMIAGVAAKRMNREQFFRAASGLDEDRLRKALWNLYWRGTAN 60
+LGPIRQPRLTVRPGRLPGMIAGVAAKRMNREQFFRAASGLDEDRLRKALWNLYWRGTAN
Sbjct 1 MLGPIRQPRLTVRPGRLPGMIAGVAAKRMNREQFFRAASGLDEDRLRKALWNLYWRGTAN 60
Query 61 MRERIEAELASAGRARPARKIKPPADPDIVGWEVDEFVSLARSGAYLGGDRRVSPRERSR 120
MRERIEAELASAGRARPARKIKPPADPDIVGWEVDEFVSLARSGAYLGGDRRVSPRERSR
Sbjct 61 MRERIEAELASAGRARPARKIKPPADPDIVGWEVDEFVSLARSGAYLGGDRRVSPRERSR 120
Query 121 WRFTFKRLAAEAQDALRAEDAEPAASALEQLIDLAREADGYDYFRSDDPVAAAGFVVSDV 180
WRFTFKRLAAEAQDALRAEDAEPAASALEQLIDLAREADGYDYFRSDDPVAAAGF
Sbjct 121 WRFTFKRLAAEAQDALRAEDAEPAASALEQLIDLAREADGYDYFRSDDPVAAAGFRRVRC 180
Query 181 AAAGHPHF 188
G P
Sbjct 181 GGGGPPTL 188
>gi|333989262|ref|YP_004521876.1| hypothetical protein JDM601_0622 [Mycobacterium sp. JDM601]
gi|333485230|gb|AEF34622.1| conserved hypothetical protein [Mycobacterium sp. JDM601]
Length=256
Score = 171 bits (433), Expect = 5e-41, Method: Compositional matrix adjust.
Identities = 105/161 (66%), Positives = 120/161 (75%), Gaps = 5/161 (3%)
Query 24 VAAKRMNREQFFRAASGLDEDRLRKALWNLYWRGTANMRERIEAELASAGRARPARKIKP 83
+A RMNREQFF A SG D+ LRKALWNLYWRGTA++R RIE ELA G RPA IKP
Sbjct 1 MAVDRMNREQFFSAMSGHDDAALRKALWNLYWRGTADVRRRIETELA--GDIRPA--IKP 56
Query 84 -PADPDIVGWEVDEFVSLARSGAYLGGDRRVSPRERSRWRFTFKRLAAEAQDALRAEDAE 142
P DP V V EFV+LAR+GAYLG DRRVSP+ER+RWRFTFK+LA A +AL A +
Sbjct 57 EPLDPQAVHDAVTEFVALARAGAYLGRDRRVSPKERTRWRFTFKQLATAAVEALHAGEPT 116
Query 143 PAASALEQLIDLAREADGYDYFRSDDPVAAAGFVVSDVAAA 183
PA +AL LIDL +A YFRS+DPV+AAGFVVSD AAA
Sbjct 117 PAITALTLLIDLILDARDTYYFRSEDPVSAAGFVVSDAAAA 157
>gi|260907600|ref|ZP_05915922.1| hypothetical protein BlinB_19847 [Brevibacterium linens BL2]
Length=299
Score = 119 bits (298), Expect = 2e-25, Method: Compositional matrix adjust.
Identities = 78/163 (48%), Positives = 103/163 (64%), Gaps = 10/163 (6%)
Query 24 VAAKRMNREQFFRAASGLDEDRLRKALWNLYWRGTANMRERIEAELASAGRARPARKIKP 83
+ AK+ +R QFFR SG D D L K LW LYWRG A RER+E EL + +
Sbjct 1 MVAKKFDRTQFFRTTSGFDRDDLEKVLWTLYWRGDARTRERVE-ELIDPSQV----TVTA 55
Query 84 PADP--DIVGWEVDEFVSLARSGAYLGGDRRVSPRERSRWRFTFKRLAAEAQDALRA--- 138
PA P ++V V EF +LAR+ AYL DRRVSP+ER+RWRFT+K A++ AL A
Sbjct 56 PAPPSAEVVRRNVKEFAALARARAYLARDRRVSPKERTRWRFTYKDHFAQSFAALSAGTG 115
Query 139 EDAEPAASALEQLIDLAREADGYDYFRSDDPVAAAGFVVSDVA 181
E+ PA A+ LI LA E +G+DYFRS+DP+ A+ V+S++
Sbjct 116 EEIRPAVEAVSTLITLACETEGFDYFRSEDPIEASKVVISEMV 158
>gi|31791792|ref|NP_854285.1| hypothetical protein Mb0626 [Mycobacterium bovis AF2122/97]
gi|57116759|ref|YP_177628.1| hypothetical protein Rv0609A [Mycobacterium tuberculosis H37Rv]
gi|121636528|ref|YP_976751.1| hypothetical protein BCG_0656 [Mycobacterium bovis BCG str. Pasteur
1173P2]
27 more sequence titles
Length=75
Score = 50.1 bits (118), Expect = 2e-04, Method: Compositional matrix adjust.
Identities = 26/35 (75%), Positives = 28/35 (80%), Gaps = 2/35 (5%)
Query 152 IDLAR--EADGYDYFRSDDPVAAAGFVVSDVAAAG 184
+ LAR EA GYDYFRSDDPVAAAGFVVS V + G
Sbjct 22 VSLARRCEAHGYDYFRSDDPVAAAGFVVSAVWSCG 56
>gi|15840010|ref|NP_335047.1| hypothetical protein MT0638.1 [Mycobacterium tuberculosis CDC1551]
gi|13880153|gb|AAK44861.1| hypothetical protein MT0638.1 [Mycobacterium tuberculosis CDC1551]
Length=57
Score = 49.3 bits (116), Expect = 4e-04, Method: Compositional matrix adjust.
Identities = 26/35 (75%), Positives = 28/35 (80%), Gaps = 2/35 (5%)
Query 152 IDLAR--EADGYDYFRSDDPVAAAGFVVSDVAAAG 184
+ LAR EA GYDYFRSDDPVAAAGFVVS V + G
Sbjct 4 VSLARRCEAHGYDYFRSDDPVAAAGFVVSAVWSCG 38
>gi|336115309|ref|YP_004570076.1| hypothetical protein BCO26_2632 [Bacillus coagulans 2-6]
gi|335368739|gb|AEH54690.1| hypothetical protein BCO26_2632 [Bacillus coagulans 2-6]
Length=337
Score = 48.1 bits (113), Expect = 7e-04, Method: Compositional matrix adjust.
Identities = 24/83 (29%), Positives = 41/83 (50%), Gaps = 1/83 (1%)
Query 93 EVDEFVSLARSGAYLGGDRRVSPRERSRWRFTFKRLAAEAQDA-LRAEDAEPAASALEQL 151
E+ +F+ A Y+ +R VS +ER +WRF K + Q + + + A LE+L
Sbjct 71 EITQFIQNAYKQNYIAPNRYVSKKERPKWRFKVKSYIKQLQQVPVEGNEGKRATDLLEKL 130
Query 152 IDLAREADGYDYFRSDDPVAAAG 174
++ GY F +D+P + G
Sbjct 131 YEMLCYGCGYYIFNTDNPFRSIG 153
>gi|255280369|ref|ZP_05344924.1| hypothetical protein BRYFOR_05702 [Bryantella formatexigens DSM
14469]
gi|255268834|gb|EET62039.1| hypothetical protein BRYFOR_05702 [Bryantella formatexigens DSM
14469]
Length=344
Score = 47.0 bits (110), Expect = 0.001, Method: Compositional matrix adjust.
Identities = 37/144 (26%), Positives = 63/144 (44%), Gaps = 3/144 (2%)
Query 42 DEDRLRKALWNLYWRGTANMRERIEAELASAGRARPARKIK--PPADPDIVGWEVDEFVS 99
D + KA Y + T + +E I+ + R A K K + + E+++F+
Sbjct 14 DRSYVEKAFAESYKQLTKSQKEEIDPVIIDILEGREAEKKKKGSAVSFEKLEQEIEDFIE 73
Query 100 LARSGAYLGGDRRVSPRERSRWRFTFKRLAAEAQDAL-RAEDAEPAASALEQLIDLAREA 158
A + Y +R + +R +WRF K E + L +E+ + A L +L L EA
Sbjct 74 NAYAQNYFAPNRVIPKSQRPKWRFMVKNFIKELEKILVESENYDRAVKLLTELYKLICEA 133
Query 159 DGYDYFRSDDPVAAAGFVVSDVAA 182
Y F +DD + G+ D+ A
Sbjct 134 CNYYLFSTDDAFRSIGWSQPDLFA 157
>gi|289441999|ref|ZP_06431743.1| conserved hypothetical protein [Mycobacterium tuberculosis T46]
gi|289568544|ref|ZP_06448771.1| conserved hypothetical protein [Mycobacterium tuberculosis T17]
gi|289749108|ref|ZP_06508486.1| conserved hypothetical protein [Mycobacterium tuberculosis T92]
gi|289414918|gb|EFD12158.1| conserved hypothetical protein [Mycobacterium tuberculosis T46]
gi|289542298|gb|EFD45946.1| conserved hypothetical protein [Mycobacterium tuberculosis T17]
gi|289689695|gb|EFD57124.1| conserved hypothetical protein [Mycobacterium tuberculosis T92]
Length=75
Score = 47.0 bits (110), Expect = 0.002, Method: Compositional matrix adjust.
Identities = 25/35 (72%), Positives = 27/35 (78%), Gaps = 2/35 (5%)
Query 152 IDLAR--EADGYDYFRSDDPVAAAGFVVSDVAAAG 184
+ LAR EA GYDYFRS DPVAAAGFVVS V + G
Sbjct 22 VSLARRCEAHGYDYFRSVDPVAAAGFVVSAVWSCG 56
>gi|229542672|ref|ZP_04431732.1| hypothetical protein BcoaDRAFT_5242 [Bacillus coagulans 36D1]
gi|229327092|gb|EEN92767.1| hypothetical protein BcoaDRAFT_5242 [Bacillus coagulans 36D1]
Length=340
Score = 45.1 bits (105), Expect = 0.007, Method: Compositional matrix adjust.
Identities = 23/83 (28%), Positives = 40/83 (49%), Gaps = 1/83 (1%)
Query 93 EVDEFVSLARSGAYLGGDRRVSPRERSRWRFTFKRLAAEAQDA-LRAEDAEPAASALEQL 151
E+ +F+ A Y+ +R VS +ER +WRF K + Q + + + A L+ L
Sbjct 71 EITQFIQNAYKQNYIAPNRYVSKKERPKWRFKVKSYIKQLQQVPVEGNEGKRATDLLDAL 130
Query 152 IDLAREADGYDYFRSDDPVAAAG 174
++ GY F +D+P + G
Sbjct 131 YEMLCYGCGYYIFNTDNPFRSIG 153
>gi|337769103|emb|CCB77816.1| putative FMN-dependent monooxygenase [Streptomyces cattleya NRRL
8057]
Length=349
Score = 41.2 bits (95), Expect = 0.095, Method: Compositional matrix adjust.
Identities = 55/162 (34%), Positives = 75/162 (47%), Gaps = 24/162 (14%)
Query 3 GPIRQPRLTVRPGR--LPGMIAGVAAKRMNREQFFRAASGLDEDRLRKALWNLYWRGTAN 60
GP + +LTV P R +P IA + K N EQ A G AL L + +
Sbjct 146 GPGKPLKLTVHPVREYIPLYIAAIGPK--NLEQTGEIADG--------AL--LIFPSAEH 193
Query 61 MRERIEAELASAGRARPARKIK----PPADPDIVGWEVDEFVSLAR--SGAYLGG--DRR 112
+ E A L AGR R R ++ P P VG +VD L R + Y+GG R+
Sbjct 194 LAETALAPL-RAGRERAGRTLEGFDVCPTLPIAVGEDVDALADLFRPYTALYVGGMGSRK 252
Query 113 VSPRERSRWRFTFKRLAAEAQDALRAEDAEPAASAL-EQLID 153
+ R R +++ AAE QD A D E AA+A+ +LID
Sbjct 253 QNFYNRLAQRMGYEKAAAEIQDKYLAGDKEGAAAAVPRELID 294
>gi|124006831|ref|ZP_01691661.1| hypothetical protein M23134_06531 [Microscilla marina ATCC 23134]
gi|123987512|gb|EAY27221.1| hypothetical protein M23134_06531 [Microscilla marina ATCC 23134]
Length=336
Score = 38.5 bits (88), Expect = 0.57, Method: Compositional matrix adjust.
Identities = 22/83 (27%), Positives = 38/83 (46%), Gaps = 1/83 (1%)
Query 93 EVDEFVSLARSGAYLGGDRRVSPRERSRWRFTFKRLAAEAQDALRAE-DAEPAASALEQL 151
E + F+ A+ G Y+ ++ V ++RS+WRF K+L R + D L L
Sbjct 65 ETEVFILNAKEGNYIKPNQMVPKKDRSKWRFLVKQLYKALSKHNRPDKDLGLQVQLLSGL 124
Query 152 IDLAREADGYDYFRSDDPVAAAG 174
+ +A+ YF + P + G
Sbjct 125 YGVLCQAESLSYFTTQSPFNSVG 147
>gi|317038352|ref|XP_001402108.2| pH-response regulator protein palF/RIM8 [Aspergillus niger CBS
513.88]
Length=739
Score = 34.7 bits (78), Expect = 7.1, Method: Compositional matrix adjust.
Identities = 25/90 (28%), Positives = 41/90 (46%), Gaps = 5/90 (5%)
Query 37 AASGLDEDRLRK-----ALWNLYWRGTANMRERIEAELASAGRARPARKIKPPADPDIVG 91
A + LD D++R+ A+ GT + + ++A+ + A P +PPA PD
Sbjct 469 AGNILDTDQIRREKGVVAVMFEVVIGTRDSQRGVKAKERTPSTAAPVDPNQPPAGPDGET 528
Query 92 WEVDEFVSLARSGAYLGGDRRVSPRERSRW 121
W D+ + G Y+ + PRE S W
Sbjct 529 WTTDQSPTPNAEGEYVAQEDYGFPREPSHW 558
>gi|134074717|emb|CAK38902.1| unnamed protein product [Aspergillus niger]
Length=759
Score = 34.7 bits (78), Expect = 7.4, Method: Compositional matrix adjust.
Identities = 25/90 (28%), Positives = 41/90 (46%), Gaps = 5/90 (5%)
Query 37 AASGLDEDRLRK-----ALWNLYWRGTANMRERIEAELASAGRARPARKIKPPADPDIVG 91
A + LD D++R+ A+ GT + + ++A+ + A P +PPA PD
Sbjct 489 AGNILDTDQIRREKGVVAVMFEVVIGTRDSQRGVKAKERTPSTAAPVDPNQPPAGPDGET 548
Query 92 WEVDEFVSLARSGAYLGGDRRVSPRERSRW 121
W D+ + G Y+ + PRE S W
Sbjct 549 WTTDQSPTPNAEGEYVAQEDYGFPREPSHW 578
>gi|310791717|gb|EFQ27244.1| NACHT and TPR domain-containing protein [Glomerella graminicola
M1.001]
Length=1442
Score = 34.7 bits (78), Expect = 8.5, Method: Composition-based stats.
Identities = 29/96 (31%), Positives = 44/96 (46%), Gaps = 12/96 (12%)
Query 93 EVDEFVSLARSGAYLGGDRRVSPRERSRWRFTFKRLAA-------EAQDALRAEDAEPAA 145
E D +S+ S ++ R S R + +FK+LA E LR+E EP
Sbjct 1138 EYDNAISIQESICFVEYKARGSLAVRVEYIASFKQLARAYALKALEVDQVLRSETVEPWV 1197
Query 146 SALEQLIDLAREADGYDYFRSDDPVAAAGFVVSDVA 181
LEQL++ R+ Y S+ P+ AGF ++ A
Sbjct 1198 RKLEQLMEQQRK-----YQNSNVPLHMAGFDSNEAA 1228
Lambda K H
0.321 0.136 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 217214446392
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Sep 5, 2011 4:36 AM
Number of letters in database: 5,219,829,388
Number of sequences in database: 15,229,318
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40