BLASTP 2.2.25+
Reference:
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database
search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
15,229,318 sequences; 5,219,829,388 total letters
Query= Rv0961
Length=115
Score E
Sequences producing significant alignments: (Bits) Value
gi|15608101|ref|NP_215476.1| integral membrane protein [Mycobact... 224 2e-57
gi|148660741|ref|YP_001282264.1| putative integral membrane prot... 221 3e-56
gi|167967734|ref|ZP_02550011.1| hypothetical integral membrane p... 210 5e-53
gi|340625974|ref|YP_004744426.1| putative integral membrane prot... 203 7e-51
gi|313657797|ref|ZP_07814677.1| putative integral membrane prote... 179 1e-43
gi|289749489|ref|ZP_06508867.1| integral membrane protein [Mycob... 97.1 9e-19
gi|296140479|ref|YP_003647722.1| hypothetical protein Tpau_2785 ... 40.4 0.083
gi|334128953|ref|ZP_08502829.1| sulfatase [Centipeda periodontii... 35.8 2.3
>gi|15608101|ref|NP_215476.1| integral membrane protein [Mycobacterium tuberculosis H37Rv]
gi|15840386|ref|NP_335423.1| hypothetical protein MT0989 [Mycobacterium tuberculosis CDC1551]
gi|31792150|ref|NP_854643.1| integral membrane protein [Mycobacterium bovis AF2122/97]
42 more sequence titles
Length=115
Score = 224 bits (572), Expect = 2e-57, Method: Compositional matrix adjust.
Identities = 114/115 (99%), Positives = 115/115 (100%), Gaps = 0/115 (0%)
Query 1 VRVPSQWMISSRVTVAWNIVGYLVYAALAFVGGFAVWFSLFFAMATDGCHDSACDASYHV 60
+RVPSQWMISSRVTVAWNIVGYLVYAALAFVGGFAVWFSLFFAMATDGCHDSACDASYHV
Sbjct 1 MRVPSQWMISSRVTVAWNIVGYLVYAALAFVGGFAVWFSLFFAMATDGCHDSACDASYHV 60
Query 61 FPAMVTMWIGVGAVLLLTLVVMVRNSSRGNVVIGWPFVGLLALGLVYVAADAVLH 115
FPAMVTMWIGVGAVLLLTLVVMVRNSSRGNVVIGWPFVGLLALGLVYVAADAVLH
Sbjct 61 FPAMVTMWIGVGAVLLLTLVVMVRNSSRGNVVIGWPFVGLLALGLVYVAADAVLH 115
>gi|148660741|ref|YP_001282264.1| putative integral membrane protein [Mycobacterium tuberculosis
H37Ra]
gi|297730469|ref|ZP_06959587.1| hypothetical protein MtubKR_05226 [Mycobacterium tuberculosis
KZN R506]
gi|298524454|ref|ZP_07011863.1| conserved hypothetical protein [Mycobacterium tuberculosis 94_M4241A]
29 more sequence titles
Length=113
Score = 221 bits (563), Expect = 3e-56, Method: Compositional matrix adjust.
Identities = 112/113 (99%), Positives = 113/113 (100%), Gaps = 0/113 (0%)
Query 3 VPSQWMISSRVTVAWNIVGYLVYAALAFVGGFAVWFSLFFAMATDGCHDSACDASYHVFP 62
+PSQWMISSRVTVAWNIVGYLVYAALAFVGGFAVWFSLFFAMATDGCHDSACDASYHVFP
Sbjct 1 MPSQWMISSRVTVAWNIVGYLVYAALAFVGGFAVWFSLFFAMATDGCHDSACDASYHVFP 60
Query 63 AMVTMWIGVGAVLLLTLVVMVRNSSRGNVVIGWPFVGLLALGLVYVAADAVLH 115
AMVTMWIGVGAVLLLTLVVMVRNSSRGNVVIGWPFVGLLALGLVYVAADAVLH
Sbjct 61 AMVTMWIGVGAVLLLTLVVMVRNSSRGNVVIGWPFVGLLALGLVYVAADAVLH 113
>gi|167967734|ref|ZP_02550011.1| hypothetical integral membrane protein [Mycobacterium tuberculosis
H37Ra]
gi|254549942|ref|ZP_05140389.1| hypothetical protein Mtube_05683 [Mycobacterium tuberculosis
'98-R604 INH-RIF-EM']
gi|297633484|ref|ZP_06951264.1| hypothetical protein MtubK4_05146 [Mycobacterium tuberculosis
KZN 4207]
Length=108
Score = 210 bits (535), Expect = 5e-53, Method: Compositional matrix adjust.
Identities = 108/108 (100%), Positives = 108/108 (100%), Gaps = 0/108 (0%)
Query 8 MISSRVTVAWNIVGYLVYAALAFVGGFAVWFSLFFAMATDGCHDSACDASYHVFPAMVTM 67
MISSRVTVAWNIVGYLVYAALAFVGGFAVWFSLFFAMATDGCHDSACDASYHVFPAMVTM
Sbjct 1 MISSRVTVAWNIVGYLVYAALAFVGGFAVWFSLFFAMATDGCHDSACDASYHVFPAMVTM 60
Query 68 WIGVGAVLLLTLVVMVRNSSRGNVVIGWPFVGLLALGLVYVAADAVLH 115
WIGVGAVLLLTLVVMVRNSSRGNVVIGWPFVGLLALGLVYVAADAVLH
Sbjct 61 WIGVGAVLLLTLVVMVRNSSRGNVVIGWPFVGLLALGLVYVAADAVLH 108
>gi|340625974|ref|YP_004744426.1| putative integral membrane protein [Mycobacterium canettii CIPT
140010059]
gi|340004164|emb|CCC43302.1| putative integral membrane protein [Mycobacterium canettii CIPT
140010059]
Length=115
Score = 203 bits (516), Expect = 7e-51, Method: Compositional matrix adjust.
Identities = 112/115 (98%), Positives = 114/115 (99%), Gaps = 0/115 (0%)
Query 1 VRVPSQWMISSRVTVAWNIVGYLVYAALAFVGGFAVWFSLFFAMATDGCHDSACDASYHV 60
+RVPSQW ISSRVTVAWNIVGYL+YAALAFVGGFAVWFSLFFAMATDGCHDSACDASYHV
Sbjct 1 MRVPSQWTISSRVTVAWNIVGYLLYAALAFVGGFAVWFSLFFAMATDGCHDSACDASYHV 60
Query 61 FPAMVTMWIGVGAVLLLTLVVMVRNSSRGNVVIGWPFVGLLALGLVYVAADAVLH 115
FPAMVTMWIGVGAVLLLTLVVMVRNSSRGNVVIGWPFVGLLALGLVYVAADAVLH
Sbjct 61 FPAMVTMWIGVGAVLLLTLVVMVRNSSRGNVVIGWPFVGLLALGLVYVAADAVLH 115
>gi|313657797|ref|ZP_07814677.1| putative integral membrane protein [Mycobacterium tuberculosis
KZN V2475]
Length=92
Score = 179 bits (454), Expect = 1e-43, Method: Compositional matrix adjust.
Identities = 91/92 (99%), Positives = 92/92 (100%), Gaps = 0/92 (0%)
Query 24 VYAALAFVGGFAVWFSLFFAMATDGCHDSACDASYHVFPAMVTMWIGVGAVLLLTLVVMV 83
+YAALAFVGGFAVWFSLFFAMATDGCHDSACDASYHVFPAMVTMWIGVGAVLLLTLVVMV
Sbjct 1 MYAALAFVGGFAVWFSLFFAMATDGCHDSACDASYHVFPAMVTMWIGVGAVLLLTLVVMV 60
Query 84 RNSSRGNVVIGWPFVGLLALGLVYVAADAVLH 115
RNSSRGNVVIGWPFVGLLALGLVYVAADAVLH
Sbjct 61 RNSSRGNVVIGWPFVGLLALGLVYVAADAVLH 92
>gi|289749489|ref|ZP_06508867.1| integral membrane protein [Mycobacterium tuberculosis T92]
gi|289690076|gb|EFD57505.1| integral membrane protein [Mycobacterium tuberculosis T92]
Length=80
Score = 97.1 bits (240), Expect = 9e-19, Method: Compositional matrix adjust.
Identities = 56/62 (91%), Positives = 57/62 (92%), Gaps = 0/62 (0%)
Query 44 MATDGCHDSACDASYHVFPAMVTMWIGVGAVLLLTLVVMVRNSSRGNVVIGWPFVGLLAL 103
MATDGCHDSACDASYHVFPAMVTMWIGVGAVLLLTLVVMVRNSSRGNVVIGWP +G L
Sbjct 1 MATDGCHDSACDASYHVFPAMVTMWIGVGAVLLLTLVVMVRNSSRGNVVIGWPLLGCWRL 60
Query 104 GL 105
L
Sbjct 61 AL 62
>gi|296140479|ref|YP_003647722.1| hypothetical protein Tpau_2785 [Tsukamurella paurometabola DSM
20162]
gi|296028613|gb|ADG79383.1| hypothetical protein Tpau_2785 [Tsukamurella paurometabola DSM
20162]
Length=134
Score = 40.4 bits (93), Expect = 0.083, Method: Compositional matrix adjust.
Identities = 24/70 (35%), Positives = 35/70 (50%), Gaps = 1/70 (1%)
Query 32 GGFAVWFSLFFAMATDGCH-DSACDASYHVFPAMVTMWIGVGAVLLLTLVVMVRNSSRGN 90
GG +FSLFF M +GC + C V A T+W GV L++ L+ + + R
Sbjct 45 GGICAFFSLFFGMNLNGCQANRDCPQWDQVLRATWTVWGGVTLALVVALIGTIVCAVRRR 104
Query 91 VVIGWPFVGL 100
V WP +G+
Sbjct 105 YVSYWPLIGM 114
>gi|334128953|ref|ZP_08502829.1| sulfatase [Centipeda periodontii DSM 2778]
gi|333385980|gb|EGK57205.1| sulfatase [Centipeda periodontii DSM 2778]
Length=673
Score = 35.8 bits (81), Expect = 2.3, Method: Composition-based stats.
Identities = 22/61 (37%), Positives = 31/61 (51%), Gaps = 2/61 (3%)
Query 17 WNIVGYLVYAALAFVGGFAVWF--SLFFAMATDGCHDSACDASYHVFPAMVTMWIGVGAV 74
W ++GYL Y AL F+ G AV SL ++ TDG A +A V ++ W+ G
Sbjct 147 WLLLGYLPYLALCFLAGNAVLSLPSLSYSTVTDGAGQYAVNALVFVLSIVLFYWLRYGGT 206
Query 75 L 75
L
Sbjct 207 L 207
Lambda K H
0.331 0.140 0.465
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 131043835296
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Sep 5, 2011 4:36 AM
Number of letters in database: 5,219,829,388
Number of sequences in database: 15,229,318
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40