BLASTP 2.2.25+
Reference:
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database
search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
15,229,318 sequences; 5,219,829,388 total letters
Query= Rv1089A
Length=34
Score E
Sequences producing significant alignments: (Bits) Value
gi|31792282|ref|NP_854775.1| endo-1,4-beta-glucanase [Mycobacter... 72.4 2e-11
gi|240169119|ref|ZP_04747778.1| glycoside hydrolase family prote... 37.7 0.55
gi|240171553|ref|ZP_04750212.1| endo-1,4-beta-glucanase [Mycobac... 34.7 4.6
gi|9651813|gb|AAF91283.1|AF233376_1 cellulase 12A [Streptomyces ... 34.7 4.7
gi|29726916|pdb|1OA4|A Chain A, Comparison Of Family 12 Glycosid... 34.7 4.8
>gi|31792282|ref|NP_854775.1| endo-1,4-beta-glucanase [Mycobacterium bovis AF2122/97]
gi|57116825|ref|YP_177638.1| endo-1,4-beta-glucanase [Mycobacterium tuberculosis H37Rv]
gi|121637020|ref|YP_977243.1| putative cellulase celA2a [Mycobacterium bovis BCG str. Pasteur
1173P2]
45 more sequence titles
Length=34
Score = 72.4 bits (176), Expect = 2e-11, Method: Compositional matrix adjust.
Identities = 34/34 (100%), Positives = 34/34 (100%), Gaps = 0/34 (0%)
Query 1 MNGAAPTNGAPLSYPSICEGVHWGHLVGGHQPAY 34
MNGAAPTNGAPLSYPSICEGVHWGHLVGGHQPAY
Sbjct 1 MNGAAPTNGAPLSYPSICEGVHWGHLVGGHQPAY 34
>gi|240169119|ref|ZP_04747778.1| glycoside hydrolase family protein [Mycobacterium kansasii ATCC
12478]
Length=819
Score = 37.7 bits (86), Expect = 0.55, Method: Compositional matrix adjust.
Identities = 17/23 (74%), Positives = 20/23 (87%), Gaps = 0/23 (0%)
Query 2 NGAAPTNGAPLSYPSICEGVHWG 24
NG+APTNGAPL YPSI +G H+G
Sbjct 640 NGSAPTNGAPLGYPSIYDGWHYG 662
>gi|240171553|ref|ZP_04750212.1| endo-1,4-beta-glucanase [Mycobacterium kansasii ATCC 12478]
Length=521
Score = 34.7 bits (78), Expect = 4.6, Method: Compositional matrix adjust.
Identities = 15/24 (63%), Positives = 20/24 (84%), Gaps = 0/24 (0%)
Query 2 NGAAPTNGAPLSYPSICEGVHWGH 25
+G+APTNGAPLSYPS+ G H+ +
Sbjct 346 DGSAPTNGAPLSYPSVYLGCHYSN 369
>gi|9651813|gb|AAF91283.1|AF233376_1 cellulase 12A [Streptomyces sp. 11AG8]
Length=371
Score = 34.7 bits (78), Expect = 4.7, Method: Compositional matrix adjust.
Identities = 14/24 (59%), Positives = 20/24 (84%), Gaps = 0/24 (0%)
Query 2 NGAAPTNGAPLSYPSICEGVHWGH 25
+G+ PTNGAP SYPS+ +G H+G+
Sbjct 76 DGSVPTNGAPKSYPSVYDGCHYGN 99
>gi|29726916|pdb|1OA4|A Chain A, Comparison Of Family 12 Glycoside Hydrolases And Recruited
Substitutions Important For Thermal Stability
Length=222
Score = 34.7 bits (78), Expect = 4.8, Method: Compositional matrix adjust.
Identities = 14/24 (59%), Positives = 20/24 (84%), Gaps = 0/24 (0%)
Query 2 NGAAPTNGAPLSYPSICEGVHWGH 25
+G+ PTNGAP SYPS+ +G H+G+
Sbjct 45 DGSVPTNGAPKSYPSVYDGCHYGN 68
Lambda K H
0.316 0.138 0.484
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 132547865944
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Sep 5, 2011 4:36 AM
Number of letters in database: 5,219,829,388
Number of sequences in database: 15,229,318
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40