BLASTP 2.2.25+
Reference:
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database
search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
15,229,318 sequences; 5,219,829,388 total letters
Query= Rv1116A
Length=91
Score E
Sequences producing significant alignments: (Bits) Value
gi|15840554|ref|NP_335591.1| hypothetical protein MT1148 [Mycoba... 179 9e-44
gi|167969252|ref|ZP_02551529.1| hypothetical protein MtubH3_1498... 177 3e-43
gi|340626131|ref|YP_004744583.1| hypothetical protein MCAN_11271... 177 4e-43
gi|254550114|ref|ZP_05140561.1| hypothetical protein Mtube_06599... 131 3e-29
gi|298525144|ref|ZP_07012553.1| pe family protein [Mycobacterium... 90.9 6e-17
gi|15841102|ref|NP_336139.1| PE family protein [Mycobacterium tu... 90.9 6e-17
gi|183982467|ref|YP_001850758.1| PE family protein [Mycobacteriu... 77.8 5e-13
gi|118617271|ref|YP_905603.1| PE family protein [Mycobacterium u... 77.8 5e-13
gi|240168031|ref|ZP_04746690.1| PE family protein [Mycobacterium... 58.2 4e-07
gi|183982472|ref|YP_001850763.1| PE-PGRS family protein [Mycobac... 43.1 0.015
gi|118617277|ref|YP_905609.1| PE-PGRS family protein [Mycobacter... 42.0 0.030
gi|183982473|ref|YP_001850764.1| PE-PGRS family protein [Mycobac... 41.2 0.058
gi|183983477|ref|YP_001851768.1| PE-PGRS family protein [Mycobac... 38.5 0.34
gi|240173029|ref|ZP_04751687.1| PE-PGRS family protein [Mycobact... 37.0 0.96
gi|183982975|ref|YP_001851266.1| PE-PGRS family protein [Mycobac... 36.6 1.2
gi|339477613|gb|EGP92704.1| Hypothetical protein MYCGRDRAFT_6505... 35.8 2.2
gi|310766712|gb|ADP11662.1| Mrr restriction system protein (EcoK... 35.0 3.6
gi|240169291|ref|ZP_04747950.1| PE family protein [Mycobacterium... 33.9 7.2
gi|183980146|ref|YP_001848437.1| PE family protein [Mycobacteriu... 33.9 7.7
gi|240170011|ref|ZP_04748670.1| PE-PGRS family protein [Mycobact... 33.9 7.7
>gi|15840554|ref|NP_335591.1| hypothetical protein MT1148 [Mycobacterium tuberculosis CDC1551]
gi|31792310|ref|NP_854803.1| hypothetical protein Mb1147c [Mycobacterium bovis AF2122/97]
gi|57116831|ref|YP_177639.1| hypothetical protein Rv1116A [Mycobacterium tuberculosis H37Rv]
50 more sequence titles
Length=91
Score = 179 bits (455), Expect = 9e-44, Method: Compositional matrix adjust.
Identities = 91/91 (100%), Positives = 91/91 (100%), Gaps = 0/91 (0%)
Query 1 MGALGTVRGLQDSNTAFVGALHSGNLLGATGAVLQAPGNAVNGFLFGQTSISQSIDVSPE 60
MGALGTVRGLQDSNTAFVGALHSGNLLGATGAVLQAPGNAVNGFLFGQTSISQSIDVSPE
Sbjct 1 MGALGTVRGLQDSNTAFVGALHSGNLLGATGAVLQAPGNAVNGFLFGQTSISQSIDVSPE 60
Query 61 YGYELVAVSDPVGGTAGSARAGHGYVHADLR 91
YGYELVAVSDPVGGTAGSARAGHGYVHADLR
Sbjct 61 YGYELVAVSDPVGGTAGSARAGHGYVHADLR 91
>gi|167969252|ref|ZP_02551529.1| hypothetical protein MtubH3_14980 [Mycobacterium tuberculosis
H37Ra]
Length=91
Score = 177 bits (450), Expect = 3e-43, Method: Compositional matrix adjust.
Identities = 90/91 (99%), Positives = 90/91 (99%), Gaps = 0/91 (0%)
Query 1 MGALGTVRGLQDSNTAFVGALHSGNLLGATGAVLQAPGNAVNGFLFGQTSISQSIDVSPE 60
MG LGTVRGLQDSNTAFVGALHSGNLLGATGAVLQAPGNAVNGFLFGQTSISQSIDVSPE
Sbjct 1 MGVLGTVRGLQDSNTAFVGALHSGNLLGATGAVLQAPGNAVNGFLFGQTSISQSIDVSPE 60
Query 61 YGYELVAVSDPVGGTAGSARAGHGYVHADLR 91
YGYELVAVSDPVGGTAGSARAGHGYVHADLR
Sbjct 61 YGYELVAVSDPVGGTAGSARAGHGYVHADLR 91
>gi|340626131|ref|YP_004744583.1| hypothetical protein MCAN_11271 [Mycobacterium canettii CIPT
140010059]
gi|340004321|emb|CCC43463.1| conserved hypothetical protein (fragment) [Mycobacterium canettii
CIPT 140010059]
Length=91
Score = 177 bits (450), Expect = 4e-43, Method: Compositional matrix adjust.
Identities = 90/91 (99%), Positives = 91/91 (100%), Gaps = 0/91 (0%)
Query 1 MGALGTVRGLQDSNTAFVGALHSGNLLGATGAVLQAPGNAVNGFLFGQTSISQSIDVSPE 60
MGALGTVRGLQDSNTAFVGALHSGNLLGATGAVLQAPGNAVNGFLFGQTSISQSIDVSPE
Sbjct 1 MGALGTVRGLQDSNTAFVGALHSGNLLGATGAVLQAPGNAVNGFLFGQTSISQSIDVSPE 60
Query 61 YGYELVAVSDPVGGTAGSARAGHGYVHADLR 91
YG+ELVAVSDPVGGTAGSARAGHGYVHADLR
Sbjct 61 YGHELVAVSDPVGGTAGSARAGHGYVHADLR 91
>gi|254550114|ref|ZP_05140561.1| hypothetical protein Mtube_06599 [Mycobacterium tuberculosis
'98-R604 INH-RIF-EM']
Length=118
Score = 131 bits (330), Expect = 3e-29, Method: Compositional matrix adjust.
Identities = 66/66 (100%), Positives = 66/66 (100%), Gaps = 0/66 (0%)
Query 1 MGALGTVRGLQDSNTAFVGALHSGNLLGATGAVLQAPGNAVNGFLFGQTSISQSIDVSPE 60
MGALGTVRGLQDSNTAFVGALHSGNLLGATGAVLQAPGNAVNGFLFGQTSISQSIDVSPE
Sbjct 1 MGALGTVRGLQDSNTAFVGALHSGNLLGATGAVLQAPGNAVNGFLFGQTSISQSIDVSPE 60
Query 61 YGYELV 66
YGYELV
Sbjct 61 YGYELV 66
>gi|298525144|ref|ZP_07012553.1| pe family protein [Mycobacterium tuberculosis 94_M4241A]
gi|298494938|gb|EFI30232.1| pe family protein [Mycobacterium tuberculosis 94_M4241A]
Length=309
Score = 90.9 bits (224), Expect = 6e-17, Method: Compositional matrix adjust.
Identities = 49/72 (69%), Positives = 55/72 (77%), Gaps = 0/72 (0%)
Query 3 ALGTVRGLQDSNTAFVGALHSGNLLGATGAVLQAPGNAVNGFLFGQTSISQSIDVSPEYG 62
AL T+ LQ+S TAF GA+ SGNLLGA GA+LQAPGNAV GFLFGQT+ISQSI G
Sbjct 198 ALTTMTALQNSGTAFSGAVQSGNLLGAAGALLQAPGNAVTGFLFGQTAISQSIPGPSNLG 257
Query 63 YELVAVSDPVGG 74
YE V +S PVGG
Sbjct 258 YESVGISVPVGG 269
>gi|15841102|ref|NP_336139.1| PE family protein [Mycobacterium tuberculosis CDC1551]
gi|31792833|ref|NP_855326.1| PE family protein [Mycobacterium bovis AF2122/97]
gi|57116896|ref|YP_177825.1| PE family protein [Mycobacterium tuberculosis H37Rv]
69 more sequence titles
Length=310
Score = 90.9 bits (224), Expect = 6e-17, Method: Compositional matrix adjust.
Identities = 49/72 (69%), Positives = 55/72 (77%), Gaps = 0/72 (0%)
Query 3 ALGTVRGLQDSNTAFVGALHSGNLLGATGAVLQAPGNAVNGFLFGQTSISQSIDVSPEYG 62
AL T+ LQ+S TAF GA+ SGNLLGA GA+LQAPGNAV GFLFGQT+ISQSI G
Sbjct 199 ALTTMTALQNSGTAFSGAVQSGNLLGAAGALLQAPGNAVTGFLFGQTAISQSIPGPSNLG 258
Query 63 YELVAVSDPVGG 74
YE V +S PVGG
Sbjct 259 YESVGISVPVGG 270
>gi|183982467|ref|YP_001850758.1| PE family protein [Mycobacterium marinum M]
gi|183175793|gb|ACC40903.1| PE family protein [Mycobacterium marinum M]
Length=313
Score = 77.8 bits (190), Expect = 5e-13, Method: Compositional matrix adjust.
Identities = 44/72 (62%), Positives = 51/72 (71%), Gaps = 0/72 (0%)
Query 3 ALGTVRGLQDSNTAFVGALHSGNLLGATGAVLQAPGNAVNGFLFGQTSISQSIDVSPEYG 62
A+ T LQ S TAF GA+ SG+LLGA GA+L APG+AVNGFLFGQT+ISQ + G
Sbjct 198 AITTAAALQASGTAFSGAVQSGDLLGAAGALLTAPGSAVNGFLFGQTAISQMVPAPDGSG 257
Query 63 YELVAVSDPVGG 74
Y V VS PVGG
Sbjct 258 YTSVDVSVPVGG 269
>gi|118617271|ref|YP_905603.1| PE family protein [Mycobacterium ulcerans Agy99]
gi|118569381|gb|ABL04132.1| PE family protein [Mycobacterium ulcerans Agy99]
Length=313
Score = 77.8 bits (190), Expect = 5e-13, Method: Compositional matrix adjust.
Identities = 44/72 (62%), Positives = 51/72 (71%), Gaps = 0/72 (0%)
Query 3 ALGTVRGLQDSNTAFVGALHSGNLLGATGAVLQAPGNAVNGFLFGQTSISQSIDVSPEYG 62
A+ T LQ S TAF GA+ SG+LLGA GA+L APG+AVNGFLFGQT+ISQ + G
Sbjct 198 AITTAAALQASGTAFSGAVQSGDLLGAAGALLTAPGSAVNGFLFGQTAISQMVPAPDGSG 257
Query 63 YELVAVSDPVGG 74
Y V VS PVGG
Sbjct 258 YTSVDVSVPVGG 269
>gi|240168031|ref|ZP_04746690.1| PE family protein [Mycobacterium kansasii ATCC 12478]
Length=399
Score = 58.2 bits (139), Expect = 4e-07, Method: Compositional matrix adjust.
Identities = 34/65 (53%), Positives = 40/65 (62%), Gaps = 0/65 (0%)
Query 10 LQDSNTAFVGALHSGNLLGATGAVLQAPGNAVNGFLFGQTSISQSIDVSPEYGYELVAVS 69
LQ+S AF A+ SGN L A G + AP NAVN FLFG +I+QS+ GY VA S
Sbjct 292 LQNSAMAFGNAISSGNPLAAAGTLFFAPVNAVNSFLFGHETITQSMAAPAGLGYSDVAFS 351
Query 70 DPVGG 74
PVGG
Sbjct 352 VPVGG 356
>gi|183982472|ref|YP_001850763.1| PE-PGRS family protein [Mycobacterium marinum M]
gi|7630282|gb|AAF65168.1|AF201682_2 PE-PGRS homolog MAG24-1 [Mycobacterium marinum]
gi|183175798|gb|ACC40908.1| PE-PGRS family protein [Mycobacterium marinum M]
Length=638
Score = 43.1 bits (100), Expect = 0.015, Method: Compositional matrix adjust.
Identities = 24/55 (44%), Positives = 30/55 (55%), Gaps = 0/55 (0%)
Query 2 GALGTVRGLQDSNTAFVGALHSGNLLGATGAVLQAPGNAVNGFLFGQTSISQSID 56
G + + S TAFV A SGN+L AT AVL AP NG GQ++ +ID
Sbjct 518 GPVDAIDAFGTSATAFVTAAQSGNVLAATAAVLDAPAVVANGLFNGQSTFPLTID 572
>gi|118617277|ref|YP_905609.1| PE-PGRS family protein [Mycobacterium ulcerans Agy99]
gi|118569387|gb|ABL04138.1| PE-PGRS family protein [Mycobacterium ulcerans Agy99]
Length=556
Score = 42.0 bits (97), Expect = 0.030, Method: Compositional matrix adjust.
Identities = 24/65 (37%), Positives = 39/65 (60%), Gaps = 2/65 (3%)
Query 12 DSNTAFVGALHSGNLLGATGAVLQAPGNAVNGFLFGQTSISQSID--VSPEYGYELVAVS 69
S T F+ A+ +G++ GA GA++ AP NGFL G+ +I+ + +SP G E + +
Sbjct 437 QSATTFIDAVQAGDVAGAFGAIVDAPAVIANGFLNGEETITLDLPTYLSPISGTESLTSA 496
Query 70 DPVGG 74
P+GG
Sbjct 497 IPIGG 501
>gi|183982473|ref|YP_001850764.1| PE-PGRS family protein [Mycobacterium marinum M]
gi|7630283|gb|AAF65169.1|AF201682_3 PE-PGRS homolog MAG24-2 [Mycobacterium marinum]
gi|183175799|gb|ACC40909.1| PE-PGRS family protein [Mycobacterium marinum M]
Length=556
Score = 41.2 bits (95), Expect = 0.058, Method: Compositional matrix adjust.
Identities = 24/65 (37%), Positives = 39/65 (60%), Gaps = 2/65 (3%)
Query 12 DSNTAFVGALHSGNLLGATGAVLQAPGNAVNGFLFGQTSISQSID--VSPEYGYELVAVS 69
S T F+ A+ +G++ GA GA++ AP NGFL G+ +I+ + +SP G E + +
Sbjct 437 QSATTFIDAVQAGDVAGAFGAIVDAPAVIANGFLNGEGTITLDLPTYLSPISGTESLTSA 496
Query 70 DPVGG 74
P+GG
Sbjct 497 IPIGG 501
>gi|183983477|ref|YP_001851768.1| PE-PGRS family protein [Mycobacterium marinum M]
gi|183176803|gb|ACC41913.1| PE-PGRS family protein [Mycobacterium marinum M]
Length=533
Score = 38.5 bits (88), Expect = 0.34, Method: Compositional matrix adjust.
Identities = 22/69 (32%), Positives = 36/69 (53%), Gaps = 0/69 (0%)
Query 4 LGTVRGLQDSNTAFVGALHSGNLLGATGAVLQAPGNAVNGFLFGQTSISQSIDVSPEYGY 63
+ T+ G T GA+ +GN +GA GA+L P ++GFL G+T + + VS
Sbjct 395 IATLDGFATGLTVLGGAVATGNGVGAVGALLDMPAYVLDGFLNGETVVELRVPVSETVTI 454
Query 64 ELVAVSDPV 72
L ++ P+
Sbjct 455 PLTPLTPPI 463
>gi|240173029|ref|ZP_04751687.1| PE-PGRS family protein [Mycobacterium kansasii ATCC 12478]
Length=521
Score = 37.0 bits (84), Expect = 0.96, Method: Compositional matrix adjust.
Identities = 19/55 (35%), Positives = 32/55 (59%), Gaps = 0/55 (0%)
Query 3 ALGTVRGLQDSNTAFVGALHSGNLLGATGAVLQAPGNAVNGFLFGQTSISQSIDV 57
A+ T S +A +GA+ +G++LGA A++ AP NGFL G ++ ++ V
Sbjct 397 AVTTANAFGSSASAVIGAMQTGDMLGAVTALIDAPAVVANGFLNGHATLPLALSV 451
>gi|183982975|ref|YP_001851266.1| PE-PGRS family protein [Mycobacterium marinum M]
gi|183176301|gb|ACC41411.1| PE-PGRS family protein [Mycobacterium marinum M]
Length=443
Score = 36.6 bits (83), Expect = 1.2, Method: Compositional matrix adjust.
Identities = 21/45 (47%), Positives = 28/45 (63%), Gaps = 0/45 (0%)
Query 3 ALGTVRGLQDSNTAFVGALHSGNLLGATGAVLQAPGNAVNGFLFG 47
AL T++G S AF A+ SG++ GA GA++ AP NGFL G
Sbjct 326 ALSTLQGFGISLNAFTTAVQSGDIAGALGALVDAPAVLANGFLNG 370
>gi|339477613|gb|EGP92704.1| Hypothetical protein MYCGRDRAFT_65051 [Mycosphaerella graminicola
IPO323]
Length=1023
Score = 35.8 bits (81), Expect = 2.2, Method: Compositional matrix adjust.
Identities = 28/83 (34%), Positives = 40/83 (49%), Gaps = 13/83 (15%)
Query 7 VRGLQDSNTAFVGALHSGNLLGATGAVLQAPGNAVNGFLFGQTSISQSIDVSPEYGYELV 66
V G++ S A+V A+ LLG A++ A G+ G +FG S+S+ I VSP Y +
Sbjct 132 VTGIEGSWEAYVSAIVHDPLLGVEEAMVIA-GSDRRGSVFGLYSVSEQIGVSPWYYW--- 187
Query 67 AVSDPVGGTAGSARAGHGYVHAD 89
A S H +HAD
Sbjct 188 ---------ADSPPQKHSSIHAD 201
>gi|310766712|gb|ADP11662.1| Mrr restriction system protein (EcoKMrr) [Erwinia sp. Ejp617]
Length=329
Score = 35.0 bits (79), Expect = 3.6, Method: Composition-based stats.
Identities = 20/48 (42%), Positives = 27/48 (57%), Gaps = 0/48 (0%)
Query 25 NLLGATGAVLQAPGNAVNGFLFGQTSISQSIDVSPEYGYELVAVSDPV 72
N LG+T V Q P AVN L+G+ + + I PE + AVSDP+
Sbjct 129 NPLGSTLTVFQLPEYAVNELLYGERAGAVIISPPPEITDDEEAVSDPL 176
>gi|240169291|ref|ZP_04747950.1| PE family protein [Mycobacterium kansasii ATCC 12478]
Length=328
Score = 33.9 bits (76), Expect = 7.2, Method: Compositional matrix adjust.
Identities = 18/43 (42%), Positives = 29/43 (68%), Gaps = 0/43 (0%)
Query 13 SNTAFVGALHSGNLLGATGAVLQAPGNAVNGFLFGQTSISQSI 55
S T+FVGA+ +G+ +GA A+ AP + V+GFL G ++S +
Sbjct 220 SATSFVGAVSTGDPVGAATALFGAPAHIVDGFLNGHQTLSTQL 262
>gi|183980146|ref|YP_001848437.1| PE family protein [Mycobacterium marinum M]
gi|183173472|gb|ACC38582.1| PE family protein [Mycobacterium marinum M]
Length=324
Score = 33.9 bits (76), Expect = 7.7, Method: Compositional matrix adjust.
Identities = 17/45 (38%), Positives = 25/45 (56%), Gaps = 0/45 (0%)
Query 13 SNTAFVGALHSGNLLGATGAVLQAPGNAVNGFLFGQTSISQSIDV 57
S TA GAL +G+ +GA + P N NGFL G ++S + +
Sbjct 216 SGTAIFGALQAGDTMGAISTLADTPANIANGFLNGSQTLSMQLSL 260
>gi|240170011|ref|ZP_04748670.1| PE-PGRS family protein [Mycobacterium kansasii ATCC 12478]
Length=382
Score = 33.9 bits (76), Expect = 7.7, Method: Compositional matrix adjust.
Identities = 18/48 (38%), Positives = 29/48 (61%), Gaps = 0/48 (0%)
Query 3 ALGTVRGLQDSNTAFVGALHSGNLLGATGAVLQAPGNAVNGFLFGQTS 50
+L T+ G + A GA+ +G++ GA GA++ AP NGFL G+ +
Sbjct 265 SLSTLGGFGTAMAALTGAVQTGDVAGALGALIDAPAFVANGFLNGEVT 312
Lambda K H
0.315 0.134 0.385
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 128725229700
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Sep 5, 2011 4:36 AM
Number of letters in database: 5,219,829,388
Number of sequences in database: 15,229,318
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40