BLASTP 2.2.25+
Reference:
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database
search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
15,229,318 sequences; 5,219,829,388 total letters
Query= Rv1494
Length=100
Score E
Sequences producing significant alignments: (Bits) Value
gi|15608632|ref|NP_216010.1| hypothetical protein Rv1494 [Mycoba... 200 4e-50
gi|15840957|ref|NP_335994.1| hypothetical protein MT1541 [Mycoba... 198 2e-49
gi|121637426|ref|YP_977649.1| hypothetical protein BCG_1557 [Myc... 198 2e-49
gi|289574177|ref|ZP_06454404.1| antitoxin [Mycobacterium tubercu... 197 5e-49
gi|330914961|ref|XP_003296856.1| hypothetical protein PTT_07053 ... 35.8 2.4
gi|115345649|ref|YP_771830.1| ABC transporter, putative [Roseoba... 35.0 3.9
gi|255599200|ref|XP_002537175.1| Fusaric acid resistance protein... 34.3 6.8
gi|242060394|ref|XP_002451486.1| hypothetical protein SORBIDRAFT... 33.9 8.4
>gi|15608632|ref|NP_216010.1| hypothetical protein Rv1494 [Mycobacterium tuberculosis H37Rv]
gi|31792690|ref|NP_855183.1| hypothetical protein Mb1531 [Mycobacterium bovis AF2122/97]
gi|148661288|ref|YP_001282811.1| hypothetical protein MRA_1504 [Mycobacterium tuberculosis H37Ra]
66 more sequence titles
Length=100
Score = 200 bits (509), Expect = 4e-50, Method: Compositional matrix adjust.
Identities = 99/100 (99%), Positives = 100/100 (100%), Gaps = 0/100 (0%)
Query 1 VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDE 60
+PFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDE
Sbjct 1 MPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDE 60
Query 61 QLDAAYAAAIHDNPAYPYESEAERSAARARRNARQQRSAQ 100
QLDAAYAAAIHDNPAYPYESEAERSAARARRNARQQRSAQ
Sbjct 61 QLDAAYAAAIHDNPAYPYESEAERSAARARRNARQQRSAQ 100
>gi|15840957|ref|NP_335994.1| hypothetical protein MT1541 [Mycobacterium tuberculosis CDC1551]
gi|13881163|gb|AAK45808.1| hypothetical protein MT1541 [Mycobacterium tuberculosis CDC1551]
gi|323719943|gb|EGB29055.1| antitoxin [Mycobacterium tuberculosis CDC1551A]
Length=100
Score = 198 bits (504), Expect = 2e-49, Method: Compositional matrix adjust.
Identities = 98/100 (98%), Positives = 99/100 (99%), Gaps = 0/100 (0%)
Query 1 VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDE 60
+PFLVALSGIISGV DHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDE
Sbjct 1 MPFLVALSGIISGVHDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDE 60
Query 61 QLDAAYAAAIHDNPAYPYESEAERSAARARRNARQQRSAQ 100
QLDAAYAAAIHDNPAYPYESEAERSAARARRNARQQRSAQ
Sbjct 61 QLDAAYAAAIHDNPAYPYESEAERSAARARRNARQQRSAQ 100
>gi|121637426|ref|YP_977649.1| hypothetical protein BCG_1557 [Mycobacterium bovis BCG str. Pasteur
1173P2]
gi|224989901|ref|YP_002644588.1| hypothetical protein JTY_1532 [Mycobacterium bovis BCG str. Tokyo
172]
gi|121493073|emb|CAL71544.1| Hypothetical protein BCG_1557 [Mycobacterium bovis BCG str. Pasteur
1173P2]
gi|224773014|dbj|BAH25820.1| hypothetical protein JTY_1532 [Mycobacterium bovis BCG str. Tokyo
172]
gi|341601445|emb|CCC64118.1| hypothetical protein BCGM_1525 [Mycobacterium bovis BCG str.
Moreau RDJ]
Length=100
Score = 198 bits (503), Expect = 2e-49, Method: Compositional matrix adjust.
Identities = 98/100 (98%), Positives = 99/100 (99%), Gaps = 0/100 (0%)
Query 1 VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDE 60
+PFLVALSGII GVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDE
Sbjct 1 MPFLVALSGIILGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDE 60
Query 61 QLDAAYAAAIHDNPAYPYESEAERSAARARRNARQQRSAQ 100
QLDAAYAAAIHDNPAYPYESEAERSAARARRNARQQRSAQ
Sbjct 61 QLDAAYAAAIHDNPAYPYESEAERSAARARRNARQQRSAQ 100
>gi|289574177|ref|ZP_06454404.1| antitoxin [Mycobacterium tuberculosis K85]
gi|339631563|ref|YP_004723205.1| hypothetical protein MAF_15180 [Mycobacterium africanum GM041182]
gi|289538608|gb|EFD43186.1| antitoxin [Mycobacterium tuberculosis K85]
gi|339330919|emb|CCC26590.1| hypothetical protein MAF_15180 [Mycobacterium africanum GM041182]
Length=100
Score = 197 bits (501), Expect = 5e-49, Method: Compositional matrix adjust.
Identities = 98/100 (98%), Positives = 99/100 (99%), Gaps = 0/100 (0%)
Query 1 VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDE 60
+PFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDE
Sbjct 1 MPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDE 60
Query 61 QLDAAYAAAIHDNPAYPYESEAERSAARARRNARQQRSAQ 100
QLDAAYAAAIHDNPAYPYESEAERSAARA RNARQQRSAQ
Sbjct 61 QLDAAYAAAIHDNPAYPYESEAERSAARAWRNARQQRSAQ 100
>gi|330914961|ref|XP_003296856.1| hypothetical protein PTT_07053 [Pyrenophora teres f. teres 0-1]
gi|311330822|gb|EFQ95050.1| hypothetical protein PTT_07053 [Pyrenophora teres f. teres 0-1]
Length=1230
Score = 35.8 bits (81), Expect = 2.4, Method: Composition-based stats.
Identities = 18/53 (34%), Positives = 28/53 (53%), Gaps = 5/53 (9%)
Query 52 KRWEALHDEQLDAAYAAAIHDNPAY-----PYESEAERSAARARRNARQQRSA 99
K W D+Q D DNP+Y PYE+ +++A +AR +A+Q +A
Sbjct 123 KTWCRGEDDQEDKLQEMTQQDNPSYYPYRAPYETHKDKAARKARESAKQSGTA 175
>gi|115345649|ref|YP_771830.1| ABC transporter, putative [Roseobacter denitrificans OCh 114]
gi|115292970|gb|ABI93422.1| ABC transporter, putative [Roseobacter denitrificans OCh 114]
Length=631
Score = 35.0 bits (79), Expect = 3.9, Method: Compositional matrix adjust.
Identities = 27/73 (37%), Positives = 36/73 (50%), Gaps = 10/73 (13%)
Query 3 FL-VALSGIISGVR--DHSMTVRLDQQTRQR---LQDIVKGGYRSANAAIV----DAINK 52
FL VA S ++ G+ + +MT L Q+T QD +GG R A A + AI
Sbjct 182 FLTVAGSAVVIGLTLLNQAMTRGLAQKTLHHDAAAQDFAEGGKRGAEAVVAMGMHTAITT 241
Query 53 RWEALHDEQLDAA 65
RW LHD+ L A
Sbjct 242 RWRGLHDDALAAG 254
>gi|255599200|ref|XP_002537175.1| Fusaric acid resistance protein fusA precursor, putative [Ricinus
communis]
gi|223517231|gb|EEF25208.1| Fusaric acid resistance protein fusA precursor, putative [Ricinus
communis]
Length=450
Score = 34.3 bits (77), Expect = 6.8, Method: Compositional matrix adjust.
Identities = 14/21 (67%), Positives = 16/21 (77%), Gaps = 0/21 (0%)
Query 54 WEALHDEQLDAAYAAAIHDNP 74
WEALHDEQL+ AA+ DNP
Sbjct 32 WEALHDEQLNRLVNAALSDNP 52
>gi|242060394|ref|XP_002451486.1| hypothetical protein SORBIDRAFT_04g002725 [Sorghum bicolor]
gi|241931317|gb|EES04462.1| hypothetical protein SORBIDRAFT_04g002725 [Sorghum bicolor]
Length=604
Score = 33.9 bits (76), Expect = 8.4, Method: Composition-based stats.
Identities = 17/57 (30%), Positives = 33/57 (58%), Gaps = 2/57 (3%)
Query 4 LVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKG-GYRSANAAIVDAINKRWEALHD 59
L A+ G+I+ +R+ +R Q +++ +DI++ G R +A +D N RW + +D
Sbjct 270 LQAIDGVINNIRESVKYIRGSQSRKEKFEDIIEELGIRCRSAPQIDVAN-RWNSTYD 325
Lambda K H
0.317 0.128 0.357
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 129239199826
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Sep 5, 2011 4:36 AM
Number of letters in database: 5,219,829,388
Number of sequences in database: 15,229,318
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40