BLASTP 2.2.25+
Reference:
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database
search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
15,229,318 sequences; 5,219,829,388 total letters
Query= Rv1724c
Length=139
Score E
Sequences producing significant alignments: (Bits) Value
gi|15608862|ref|NP_216240.1| hypothetical protein Rv1724c [Mycob... 288 1e-76
gi|340626729|ref|YP_004745181.1| hypothetical protein MCAN_17351... 287 4e-76
gi|289753814|ref|ZP_06513192.1| conserved hypothetical protein [... 286 5e-76
gi|254364561|ref|ZP_04980607.1| hypothetical protein TBHG_01681 ... 285 2e-75
gi|330817203|ref|YP_004360908.1| hypothetical protein bgla_1g232... 36.6 1.2
gi|110597193|ref|ZP_01385482.1| transcription antitermination fa... 36.2 1.6
gi|294673017|ref|YP_003573633.1| hypothetical protein PRU_0242 [... 35.4 2.6
gi|221486941|gb|EEE25187.1| conserved hypothetical protein [Toxo... 35.4 2.7
gi|221506628|gb|EEE32245.1| conserved hypothetical protein [Toxo... 35.4 2.8
gi|242399492|ref|YP_002994917.1| Glutamate synthase beta chain-r... 35.4 3.0
gi|20089624|ref|NP_615699.1| hypothetical protein MA0739 [Methan... 35.4 3.1
gi|220909077|ref|YP_002484388.1| hypothetical protein Cyan7425_3... 35.4 3.2
gi|237831827|ref|XP_002365211.1| hypothetical protein, conserved... 35.4 3.2
>gi|15608862|ref|NP_216240.1| hypothetical protein Rv1724c [Mycobacterium tuberculosis H37Rv]
gi|15841186|ref|NP_336223.1| hypothetical protein MT1765 [Mycobacterium tuberculosis CDC1551]
gi|31792912|ref|NP_855405.1| hypothetical protein Mb1753c [Mycobacterium bovis AF2122/97]
42 more sequence titles
Length=139
Score = 288 bits (738), Expect = 1e-76, Method: Compositional matrix adjust.
Identities = 139/139 (100%), Positives = 139/139 (100%), Gaps = 0/139 (0%)
Query 1 MVGNEENELQDLRNLRRPCFSRAEAPIGVYNGEQAIIVYDLRPVPHWPKYWIQALAKHFQ 60
MVGNEENELQDLRNLRRPCFSRAEAPIGVYNGEQAIIVYDLRPVPHWPKYWIQALAKHFQ
Sbjct 1 MVGNEENELQDLRNLRRPCFSRAEAPIGVYNGEQAIIVYDLRPVPHWPKYWIQALAKHFQ 60
Query 61 RQLKPSPKIDISLLDDRIRFSVFVSTDVSAKDLCKLDDAVYNAVRNAGRAIENEQAALDH 120
RQLKPSPKIDISLLDDRIRFSVFVSTDVSAKDLCKLDDAVYNAVRNAGRAIENEQAALDH
Sbjct 61 RQLKPSPKIDISLLDDRIRFSVFVSTDVSAKDLCKLDDAVYNAVRNAGRAIENEQAALDH 120
Query 121 KLAEVRKRRMDTWDESYFR 139
KLAEVRKRRMDTWDESYFR
Sbjct 121 KLAEVRKRRMDTWDESYFR 139
>gi|340626729|ref|YP_004745181.1| hypothetical protein MCAN_17351 [Mycobacterium canettii CIPT
140010059]
gi|340004919|emb|CCC44067.1| hypothetical protein MCAN_17351 [Mycobacterium canettii CIPT
140010059]
Length=139
Score = 287 bits (734), Expect = 4e-76, Method: Compositional matrix adjust.
Identities = 138/139 (99%), Positives = 138/139 (99%), Gaps = 0/139 (0%)
Query 1 MVGNEENELQDLRNLRRPCFSRAEAPIGVYNGEQAIIVYDLRPVPHWPKYWIQALAKHFQ 60
MVGNEENELQDLRNLRRPCFSRAEAPIGVYNGEQAIIVYDLRPVPHWPKYWIQALAKHFQ
Sbjct 1 MVGNEENELQDLRNLRRPCFSRAEAPIGVYNGEQAIIVYDLRPVPHWPKYWIQALAKHFQ 60
Query 61 RQLKPSPKIDISLLDDRIRFSVFVSTDVSAKDLCKLDDAVYNAVRNAGRAIENEQAALDH 120
RQLKPSPKIDISLLDDRIRFSVFVSTDVSAKDLCKLDDAVYNAVRNAGRAIENEQ ALDH
Sbjct 61 RQLKPSPKIDISLLDDRIRFSVFVSTDVSAKDLCKLDDAVYNAVRNAGRAIENEQVALDH 120
Query 121 KLAEVRKRRMDTWDESYFR 139
KLAEVRKRRMDTWDESYFR
Sbjct 121 KLAEVRKRRMDTWDESYFR 139
>gi|289753814|ref|ZP_06513192.1| conserved hypothetical protein [Mycobacterium tuberculosis EAS054]
gi|289694401|gb|EFD61830.1| conserved hypothetical protein [Mycobacterium tuberculosis EAS054]
Length=139
Score = 286 bits (733), Expect = 5e-76, Method: Compositional matrix adjust.
Identities = 138/138 (100%), Positives = 138/138 (100%), Gaps = 0/138 (0%)
Query 1 MVGNEENELQDLRNLRRPCFSRAEAPIGVYNGEQAIIVYDLRPVPHWPKYWIQALAKHFQ 60
MVGNEENELQDLRNLRRPCFSRAEAPIGVYNGEQAIIVYDLRPVPHWPKYWIQALAKHFQ
Sbjct 1 MVGNEENELQDLRNLRRPCFSRAEAPIGVYNGEQAIIVYDLRPVPHWPKYWIQALAKHFQ 60
Query 61 RQLKPSPKIDISLLDDRIRFSVFVSTDVSAKDLCKLDDAVYNAVRNAGRAIENEQAALDH 120
RQLKPSPKIDISLLDDRIRFSVFVSTDVSAKDLCKLDDAVYNAVRNAGRAIENEQAALDH
Sbjct 61 RQLKPSPKIDISLLDDRIRFSVFVSTDVSAKDLCKLDDAVYNAVRNAGRAIENEQAALDH 120
Query 121 KLAEVRKRRMDTWDESYF 138
KLAEVRKRRMDTWDESYF
Sbjct 121 KLAEVRKRRMDTWDESYF 138
>gi|254364561|ref|ZP_04980607.1| hypothetical protein TBHG_01681 [Mycobacterium tuberculosis str.
Haarlem]
gi|289554504|ref|ZP_06443714.1| hypothetical protein TBXG_02254 [Mycobacterium tuberculosis KZN
605]
gi|298525222|ref|ZP_07012631.1| conserved hypothetical protein [Mycobacterium tuberculosis 94_M4241A]
27 more sequence titles
Length=138
Score = 285 bits (729), Expect = 2e-75, Method: Compositional matrix adjust.
Identities = 137/138 (99%), Positives = 138/138 (100%), Gaps = 0/138 (0%)
Query 2 VGNEENELQDLRNLRRPCFSRAEAPIGVYNGEQAIIVYDLRPVPHWPKYWIQALAKHFQR 61
+GNEENELQDLRNLRRPCFSRAEAPIGVYNGEQAIIVYDLRPVPHWPKYWIQALAKHFQR
Sbjct 1 MGNEENELQDLRNLRRPCFSRAEAPIGVYNGEQAIIVYDLRPVPHWPKYWIQALAKHFQR 60
Query 62 QLKPSPKIDISLLDDRIRFSVFVSTDVSAKDLCKLDDAVYNAVRNAGRAIENEQAALDHK 121
QLKPSPKIDISLLDDRIRFSVFVSTDVSAKDLCKLDDAVYNAVRNAGRAIENEQAALDHK
Sbjct 61 QLKPSPKIDISLLDDRIRFSVFVSTDVSAKDLCKLDDAVYNAVRNAGRAIENEQAALDHK 120
Query 122 LAEVRKRRMDTWDESYFR 139
LAEVRKRRMDTWDESYFR
Sbjct 121 LAEVRKRRMDTWDESYFR 138
>gi|330817203|ref|YP_004360908.1| hypothetical protein bgla_1g23250 [Burkholderia gladioli BSR3]
gi|327369596|gb|AEA60952.1| hypothetical protein bgla_1g23250 [Burkholderia gladioli BSR3]
Length=127
Score = 36.6 bits (83), Expect = 1.2, Method: Compositional matrix adjust.
Identities = 21/55 (39%), Positives = 30/55 (55%), Gaps = 1/55 (1%)
Query 27 IGVYNGEQAIIVYDLRPVPHWPKYWIQAL-AKHFQRQLKPSPKIDISLLDDRIRF 80
I + E ++IV PV WP +AL A+HF+ L PSP++ I R+RF
Sbjct 24 INLARHEHSVIVLPAAPVMAWPVDAFEALEARHFELLLDPSPEVVIFGSGARLRF 78
>gi|110597193|ref|ZP_01385482.1| transcription antitermination factor NusB [Chlorobium ferrooxidans
DSM 13031]
gi|110341384|gb|EAT59849.1| transcription antitermination factor NusB [Chlorobium ferrooxidans
DSM 13031]
Length=191
Score = 36.2 bits (82), Expect = 1.6, Method: Compositional matrix adjust.
Identities = 16/50 (32%), Positives = 32/50 (64%), Gaps = 0/50 (0%)
Query 83 FVSTDVSAKDLCKLDDAVYNAVRNAGRAIENEQAALDHKLAEVRKRRMDT 132
F STD S+K + + DA++N ++ G+ +N + +DH A+V+K +++
Sbjct 142 FTSTDKSSKFVNGILDAIFNELKAEGKVHKNGRGLIDHSTAKVQKPEIES 191
>gi|294673017|ref|YP_003573633.1| hypothetical protein PRU_0242 [Prevotella ruminicola 23]
gi|294472325|gb|ADE81714.1| conserved hypothetical protein [Prevotella ruminicola 23]
Length=513
Score = 35.4 bits (80), Expect = 2.6, Method: Compositional matrix adjust.
Identities = 25/76 (33%), Positives = 37/76 (49%), Gaps = 7/76 (9%)
Query 55 LAKHFQRQLKPSPKIDISLLDDRIRFSVFVSTDVSAKDLCKLDDAVYNAVRNAGRAIENE 114
L KHF R P PKI ++ DD++R ++ TD+ + L DA+ GR E
Sbjct 59 LTKHFSRLQMPVPKI-LAASDDQLR---YLQTDLGSMSLF---DAIRGGREAGGRYTLKE 111
Query 115 QAALDHKLAEVRKRRM 130
Q L + E+ K +M
Sbjct 112 QELLRRTIRELPKMQM 127
>gi|221486941|gb|EEE25187.1| conserved hypothetical protein [Toxoplasma gondii GT1]
Length=1108
Score = 35.4 bits (80), Expect = 2.7, Method: Composition-based stats.
Identities = 28/94 (30%), Positives = 44/94 (47%), Gaps = 1/94 (1%)
Query 19 CFSRAEAPIGVYNGEQAIIVYDLRPVPHWPKYWIQALAKHFQRQLKPSPKIDISLLDDRI 78
C + + + GE +IV D RPV + +QA +QR + ++SLL D++
Sbjct 1008 CLAESASVRTAGEGEYEVIVSDDRPVGAGVFWGMQAGEAFWQRPGSDAGLWEVSLLRDQV 1067
Query 79 RFSVFVSTDVSAKDLCKLDDAVYNAVRNAGRAIE 112
+ V DV K+L K N RN +A+E
Sbjct 1068 PLDMHVGGDVRVKNLMKELTECLNR-RNQKKALE 1100
>gi|221506628|gb|EEE32245.1| conserved hypothetical protein [Toxoplasma gondii VEG]
Length=1108
Score = 35.4 bits (80), Expect = 2.8, Method: Composition-based stats.
Identities = 28/94 (30%), Positives = 44/94 (47%), Gaps = 1/94 (1%)
Query 19 CFSRAEAPIGVYNGEQAIIVYDLRPVPHWPKYWIQALAKHFQRQLKPSPKIDISLLDDRI 78
C + + + GE +IV D RPV + +QA +QR + ++SLL D++
Sbjct 1008 CLAESASVRTAGEGEYEVIVSDDRPVGAGVFWGMQAGEAFWQRPGSDAGLWEVSLLRDQV 1067
Query 79 RFSVFVSTDVSAKDLCKLDDAVYNAVRNAGRAIE 112
+ V DV K+L K N RN +A+E
Sbjct 1068 PLDMHVGGDVRVKNLMKELTECLNR-RNQKKALE 1100
>gi|242399492|ref|YP_002994917.1| Glutamate synthase beta chain-related oxidoreductase, containing
2Fe- 2S and 4Fe-4S clusters [Thermococcus sibiricus MM 739]
gi|242265886|gb|ACS90568.1| Glutamate synthase beta chain-related oxidoreductase, containing
2Fe- 2S and 4Fe-4S clusters [Thermococcus sibiricus MM 739]
Length=963
Score = 35.4 bits (80), Expect = 3.0, Method: Composition-based stats.
Identities = 20/71 (29%), Positives = 37/71 (53%), Gaps = 5/71 (7%)
Query 29 VYNGEQAIIVYDLRPVPHWPKYWIQALAKHFQRQLKPSPKIDISLLDDRIRFSVFVSTDV 88
+Y+ + +++DLRP HW K + +H +R+ P++ + LLD IR S F +
Sbjct 522 IYDEDLYRVLFDLRPYNHWKKV-TEKDYEHVERK----PRVKVKLLDPEIRKSNFKEVEP 576
Query 89 SAKDLCKLDDA 99
+ + L +A
Sbjct 577 TMDEETVLTEA 587
>gi|20089624|ref|NP_615699.1| hypothetical protein MA0739 [Methanosarcina acetivorans C2A]
gi|19914545|gb|AAM04179.1| hypothetical protein (multi-domain) [Methanosarcina acetivorans
C2A]
Length=219
Score = 35.4 bits (80), Expect = 3.1, Method: Compositional matrix adjust.
Identities = 23/73 (32%), Positives = 40/73 (55%), Gaps = 6/73 (8%)
Query 3 GNEENELQDLRNLRRPCFSRAEAPIGVYNGEQAIIVYDLRPVPHWPKYWIQALAKHFQRQ 62
G E+ L+ LRN++ + A V +G+ A+ + D+ V H Y++ A K + RQ
Sbjct 19 GGMEDPLEFLRNIK------SVAVATVDDGKPAVRMSDVMLVEHEKLYFLTARGKPYYRQ 72
Query 63 LKPSPKIDISLLD 75
LK +P+I + +D
Sbjct 73 LKKNPEIALVGMD 85
>gi|220909077|ref|YP_002484388.1| hypothetical protein Cyan7425_3708 [Cyanothece sp. PCC 7425]
gi|219865688|gb|ACL46027.1| hypothetical protein Cyan7425_3708 [Cyanothece sp. PCC 7425]
Length=207
Score = 35.4 bits (80), Expect = 3.2, Method: Compositional matrix adjust.
Identities = 14/33 (43%), Positives = 23/33 (70%), Gaps = 0/33 (0%)
Query 12 LRNLRRPCFSRAEAPIGVYNGEQAIIVYDLRPV 44
L+N P FS A+ P+G+ N ++ +IV +LRP+
Sbjct 135 LKNGELPVFSAAQLPVGLTNDDKVMIVGELRPI 167
>gi|237831827|ref|XP_002365211.1| hypothetical protein, conserved [Toxoplasma gondii ME49]
gi|211962875|gb|EEA98070.1| hypothetical protein, conserved [Toxoplasma gondii ME49]
Length=1108
Score = 35.4 bits (80), Expect = 3.2, Method: Composition-based stats.
Identities = 28/94 (30%), Positives = 44/94 (47%), Gaps = 1/94 (1%)
Query 19 CFSRAEAPIGVYNGEQAIIVYDLRPVPHWPKYWIQALAKHFQRQLKPSPKIDISLLDDRI 78
C + + + GE +IV D RPV + +QA +QR + ++SLL D++
Sbjct 1008 CLAESASVRTAGEGEYEVIVSDDRPVGAGVFWGMQAGEAFWQRPGSDAGLWEVSLLRDQV 1067
Query 79 RFSVFVSTDVSAKDLCKLDDAVYNAVRNAGRAIE 112
+ V DV K+L K N RN +A+E
Sbjct 1068 PLDMHVGGDVRVKNLMKELTECLNR-RNQEKALE 1100
Lambda K H
0.321 0.136 0.416
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 131443546824
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Sep 5, 2011 4:36 AM
Number of letters in database: 5,219,829,388
Number of sequences in database: 15,229,318
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40