BLASTP 2.2.25+


Reference:
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database
search programs", Nucleic Acids Res. 25:3389-3402.



Reference for composition-based statistics:
Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.



Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
           15,229,318 sequences; 5,219,829,388 total letters



Query= Rv1814

Length=300
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

gi|15841284|ref|NP_336321.1|  sterol desaturase-related protein [...   615    2e-174
gi|15608951|ref|NP_216330.1|  membrane-bound C-5 sterol desaturas...   615    2e-174
gi|289554412|ref|ZP_06443622.1|  membrane-bound C-5 sterol desatu...   615    3e-174
gi|306779814|ref|ZP_07418151.1|  membrane-bound C-5 sterol desatu...   613    1e-173
gi|254823254|ref|ZP_05228255.1|  C-5 sterol desaturase [Mycobacte...   523    2e-146
gi|342861287|ref|ZP_08717935.1|  C-5 sterol desaturase [Mycobacte...   520    8e-146
gi|296164798|ref|ZP_06847358.1|  C-5 sterol desaturase [Mycobacte...   520    1e-145
gi|240173100|ref|ZP_04751758.1|  membrane-bound C-5 sterol desatu...   520    2e-145
gi|118466987|ref|YP_882088.1|  C-5 sterol desaturase [Mycobacteri...   506    2e-141
gi|183982698|ref|YP_001850989.1|  membrane-bound C-5 sterol desat...   484    6e-135
gi|118618451|ref|YP_906783.1|  membrane-bound C-5 sterol desatura...   481    5e-134
gi|41407623|ref|NP_960459.1|  hypothetical protein MAP1525 [Mycob...   479    2e-133
gi|336457575|gb|EGO36580.1|  sterol desaturase [Mycobacterium avi...   479    3e-133
gi|145224004|ref|YP_001134682.1|  sterol desaturase-like protein ...   459    2e-127
gi|315444336|ref|YP_004077215.1|  sterol desaturase [Mycobacteriu...   456    2e-126
gi|333990550|ref|YP_004523164.1|  membrane-bound C-5 sterol desat...   453    1e-125
gi|120404150|ref|YP_953979.1|  sterol desaturase--like protein [M...   452    2e-125
gi|118470601|ref|YP_887969.1|  C-5 sterol desaturase [Mycobacteri...   449    3e-124
gi|183984597|ref|YP_001852888.1|  membrane-bound C-5 sterol desat...   430    1e-118
gi|108799830|ref|YP_640027.1|  sterol desaturase-like protein [My...   428    6e-118
gi|126435473|ref|YP_001071164.1|  sterol desaturase-like protein ...   427    1e-117
gi|226363499|ref|YP_002781281.1|  hypothetical protein ROP_40890 ...   422    3e-116
gi|312137812|ref|YP_004005148.1|  integral membrane protein [Rhod...   421    1e-115
gi|325676085|ref|ZP_08155767.1|  C-5 sterol desaturase [Rhodococc...   419    3e-115
gi|229492567|ref|ZP_04386370.1|  C-5 sterol desaturase [Rhodococc...   405    5e-111
gi|326383892|ref|ZP_08205576.1|  fatty acid hydroxylase [Gordonia...   404    9e-111
gi|226303825|ref|YP_002763783.1|  hypothetical protein RER_03360 ...   403    2e-110
gi|343928716|ref|ZP_08768161.1|  hypothetical protein GOALK_120_0...   371    7e-101
gi|111021138|ref|YP_704110.1|  C-5 sterol desaturase [Rhodococcus...   371    9e-101
gi|262204643|ref|YP_003275851.1|  fatty acid hydroxylase [Gordoni...   357    2e-96 
gi|319949067|ref|ZP_08023163.1|  hypothetical membrane protein [D...   327    1e-87 
gi|311743305|ref|ZP_07717112.1|  C-5 sterol desaturase [Aeromicro...   315    4e-84 
gi|291454322|ref|ZP_06593712.1|  C-5 sterol desaturase [Streptomy...   313    3e-83 
gi|300784689|ref|YP_003764980.1|  sterol desaturase [Amycolatopsi...   308    7e-82 
gi|84494268|ref|ZP_00993387.1|  hypothetical protein JNB_05719 [J...   296    3e-78 
gi|291191880|gb|ADD82987.1|  unknown [Streptomyces platensis]          275    6e-72 
gi|315505770|ref|YP_004084657.1|  fatty acid hydroxylase [Micromo...   273    3e-71 
gi|302867826|ref|YP_003836463.1|  fatty acid hydroxylase [Micromo...   272    5e-71 
gi|225791059|gb|ACO31263.1|  unknown [Streptomyces platensis]          270    3e-70 
gi|326329354|ref|ZP_08195679.1|  C-5 sterol desaturase (Sterol-C5...   264    2e-68 
gi|302524753|ref|ZP_07277095.1|  predicted protein [Streptomyces ...   261    1e-67 
gi|338532042|ref|YP_004665376.1|  hypothetical protein LILAB_1194...   254    1e-65 
gi|108759252|ref|YP_635021.1|  hypothetical protein MXAN_6907 [My...   253    2e-65 
gi|291302624|ref|YP_003513902.1|  fatty acid hydroxylase [Stackeb...   253    2e-65 
gi|256389551|ref|YP_003111115.1|  fatty acid hydroxylase [Catenul...   251    7e-65 
gi|29832988|ref|NP_827622.1|  sterol desaturase-related protein [...   248    7e-64 
gi|302550498|ref|ZP_07302840.1|  fatty acid hydroxylase superfami...   248    8e-64 
gi|297198749|ref|ZP_06916146.1|  C-5 sterol desaturase [Streptomy...   246    3e-63 
gi|328881514|emb|CCA54753.1|  sterol desaturase-related protein [...   245    6e-63 
gi|54023597|ref|YP_117839.1|  hypothetical protein nfa16290 [Noca...   244    1e-62 


>gi|15841284|ref|NP_336321.1| sterol desaturase-related protein [Mycobacterium tuberculosis 
CDC1551]
 gi|148823027|ref|YP_001287781.1| membrane-bound C-5 sterol desaturase erg3 (sterol-c5-desaturase) 
[Mycobacterium tuberculosis F11]
 gi|167967391|ref|ZP_02549668.1| membrane-bound C-5 sterol desaturase erg3 (sterol-c5-desaturase) 
[Mycobacterium tuberculosis H37Ra]
 37 more sequence titles
 Length=314

 Score =  615 bits (1587),  Expect = 2e-174, Method: Compositional matrix adjust.
 Identities = 300/300 (100%), Positives = 300/300 (100%), Gaps = 0/300 (0%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI
Sbjct  15   MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  74

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
            ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA
Sbjct  75   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  134

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE
Sbjct  135  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  194

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT
Sbjct  195  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  254

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTIDKSNAAASLVTSR  300
            KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTIDKSNAAASLVTSR
Sbjct  255  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTIDKSNAAASLVTSR  314


>gi|15608951|ref|NP_216330.1| membrane-bound C-5 sterol desaturase erg3 (sterol-c5-desaturase) 
[Mycobacterium tuberculosis H37Rv]
 gi|31793003|ref|NP_855496.1| membrane-bound C-5 sterol desaturase erg3 (sterol-c5-desaturase) 
[Mycobacterium bovis AF2122/97]
 gi|121637716|ref|YP_977939.1| membrane-bound C-5 sterol desaturase erg3 [Mycobacterium bovis 
BCG str. Pasteur 1173P2]
 35 more sequence titles
 Length=300

 Score =  615 bits (1586),  Expect = 2e-174, Method: Compositional matrix adjust.
 Identities = 300/300 (100%), Positives = 300/300 (100%), Gaps = 0/300 (0%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI
Sbjct  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
            ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA
Sbjct  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE
Sbjct  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT
Sbjct  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTIDKSNAAASLVTSR  300
            KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTIDKSNAAASLVTSR
Sbjct  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTIDKSNAAASLVTSR  300


>gi|289554412|ref|ZP_06443622.1| membrane-bound C-5 sterol desaturase erg3 [Mycobacterium tuberculosis 
KZN 605]
 gi|289439044|gb|EFD21537.1| membrane-bound C-5 sterol desaturase erg3 [Mycobacterium tuberculosis 
KZN 605]
Length=304

 Score =  615 bits (1586),  Expect = 3e-174, Method: Compositional matrix adjust.
 Identities = 300/300 (100%), Positives = 300/300 (100%), Gaps = 0/300 (0%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI
Sbjct  5    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  64

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
            ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA
Sbjct  65   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  124

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE
Sbjct  125  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  184

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT
Sbjct  185  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  244

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTIDKSNAAASLVTSR  300
            KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTIDKSNAAASLVTSR
Sbjct  245  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTIDKSNAAASLVTSR  304


>gi|306779814|ref|ZP_07418151.1| membrane-bound C-5 sterol desaturase erg3 [Mycobacterium tuberculosis 
SUMu002]
 gi|306788917|ref|ZP_07427239.1| membrane-bound C-5 sterol desaturase erg3 [Mycobacterium tuberculosis 
SUMu004]
 gi|306793253|ref|ZP_07431555.1| membrane-bound C-5 sterol desaturase erg3 [Mycobacterium tuberculosis 
SUMu005]
 gi|308327274|gb|EFP16125.1| membrane-bound C-5 sterol desaturase erg3 [Mycobacterium tuberculosis 
SUMu002]
 gi|308334540|gb|EFP23391.1| membrane-bound C-5 sterol desaturase erg3 [Mycobacterium tuberculosis 
SUMu004]
 gi|308338366|gb|EFP27217.1| membrane-bound C-5 sterol desaturase erg3 [Mycobacterium tuberculosis 
SUMu005]
Length=314

 Score =  613 bits (1581),  Expect = 1e-173, Method: Compositional matrix adjust.
 Identities = 299/300 (99%), Positives = 299/300 (99%), Gaps = 0/300 (0%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI
Sbjct  15   MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  74

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
            ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA
Sbjct  75   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  134

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE
Sbjct  135  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  194

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT
Sbjct  195  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  254

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTIDKSNAAASLVTSR  300
            KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPP WEPRTIDKSNAAASLVTSR
Sbjct  255  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPDWEPRTIDKSNAAASLVTSR  314


>gi|254823254|ref|ZP_05228255.1| C-5 sterol desaturase [Mycobacterium intracellulare ATCC 13950]
Length=311

 Score =  523 bits (1346),  Expect = 2e-146, Method: Compositional matrix adjust.
 Identities = 252/299 (85%), Positives = 274/299 (92%), Gaps = 3/299 (1%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            MRDPVL AIP FLLLL LEWTAARKLE I   A    RP SGA+ TRDS+ SISMGLVS+
Sbjct  15   MRDPVLLAIPFFLLLLTLEWTAARKLEHIAADAA---RPPSGAHRTRDSLTSISMGLVSV  71

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
            ATTAGWK+LALLGYAAIYAY+APW LS  RWYTWVIAI+GVDLLYY+YHRIAHRVRLIWA
Sbjct  72   ATTAGWKTLALLGYAAIYAYVAPWHLSVARWYTWVIAILGVDLLYYAYHRIAHRVRLIWA  131

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            THQAHHSSEY+NFATALRQKWNNSGEILMW+PLPL+G+PPWMVF S+S+NLIYQFW+HTE
Sbjct  132  THQAHHSSEYYNFATALRQKWNNSGEILMWIPLPLLGIPPWMVFFSFSVNLIYQFWIHTE  191

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            RID+LPR FEFVFNTPSHHRVHHGMD VYLDKNYGGI IIWDRLFG+FQPELFRPHYGLT
Sbjct  192  RIDKLPRPFEFVFNTPSHHRVHHGMDKVYLDKNYGGIFIIWDRLFGTFQPELFRPHYGLT  251

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTIDKSNAAASLVTS  299
            K+VDTFNIWKLQTREYVAI RDWR+ATRLRDRLGYVFGPPGW PRT  +++AAA+LVTS
Sbjct  252  KQVDTFNIWKLQTREYVAIARDWRTATRLRDRLGYVFGPPGWAPRTAGQTDAAAALVTS  310


>gi|342861287|ref|ZP_08717935.1| C-5 sterol desaturase [Mycobacterium colombiense CECT 3035]
 gi|342131187|gb|EGT84468.1| C-5 sterol desaturase [Mycobacterium colombiense CECT 3035]
Length=300

 Score =  520 bits (1340),  Expect = 8e-146, Method: Compositional matrix adjust.
 Identities = 249/299 (84%), Positives = 270/299 (91%), Gaps = 0/299 (0%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            MRDPVLFAIP FLLLL LEWTAARKLE +   A    RPASGA+ TRDSVASISMGLVS+
Sbjct  1    MRDPVLFAIPFFLLLLTLEWTAARKLEHLTAEAADTARPASGAHRTRDSVASISMGLVSV  60

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
            ATTAGWK+LALLGYAAIYAY+APW LSA  WYTWV+AI+GVDLLYY+YHRIAHRVRLIWA
Sbjct  61   ATTAGWKTLALLGYAAIYAYVAPWHLSARHWYTWVVAILGVDLLYYAYHRIAHRVRLIWA  120

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            THQAHHSSEY+NFATALRQKWNNSGEILMW+PLPL+G+PPWMVF S+SLNLIYQFW+HTE
Sbjct  121  THQAHHSSEYYNFATALRQKWNNSGEILMWIPLPLLGIPPWMVFFSFSLNLIYQFWIHTE  180

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            RID+LPR FEF+FNTPSHHRVHHGMD VYLDKNYGGI I+WDRLFG+FQ ELFRPHYGLT
Sbjct  181  RIDKLPRPFEFLFNTPSHHRVHHGMDKVYLDKNYGGIFIVWDRLFGTFQAELFRPHYGLT  240

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTIDKSNAAASLVTS  299
            KRVDTFNIW LQTREYVA+ RDWRSATRLRDRLGYVFGPPGW PR    + A A +VTS
Sbjct  241  KRVDTFNIWTLQTREYVAMARDWRSATRLRDRLGYVFGPPGWLPRAARHAEAGAPVVTS  299


>gi|296164798|ref|ZP_06847358.1| C-5 sterol desaturase [Mycobacterium parascrofulaceum ATCC BAA-614]
 gi|295899852|gb|EFG79298.1| C-5 sterol desaturase [Mycobacterium parascrofulaceum ATCC BAA-614]
Length=312

 Score =  520 bits (1338),  Expect = 1e-145, Method: Compositional matrix adjust.
 Identities = 250/299 (84%), Positives = 272/299 (91%), Gaps = 4/299 (1%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            MRDPVL AIP FLLLL LEWTAARKLES+    T + RP SGAYLTRDS+ASISMGLVS+
Sbjct  17   MRDPVLLAIPFFLLLLTLEWTAARKLESL----TKEARPVSGAYLTRDSLASISMGLVSV  72

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
             TTA WK+LAL GYAA++AY+APW LSA RWYTWVIA+VGVDLLYY+YHRIAHRVRLIWA
Sbjct  73   GTTAAWKTLALFGYAALFAYVAPWHLSAARWYTWVIALVGVDLLYYAYHRIAHRVRLIWA  132

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPL+G+PPWMVF S+S++LIYQFWVHTE
Sbjct  133  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLLGIPPWMVFFSFSISLIYQFWVHTE  192

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            RID+LPR FEFVFNTPSHHRVHHGMD +YLDKNYGGILI+WDRLFGSFQ ELFRPHYGLT
Sbjct  193  RIDKLPRPFEFVFNTPSHHRVHHGMDQLYLDKNYGGILILWDRLFGSFQAELFRPHYGLT  252

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTIDKSNAAASLVTS  299
            KRVDTFNIWKLQTREYVAI  DWRSATRLRDRLGYVFGPPGW PRT D+ +AA  + +S
Sbjct  253  KRVDTFNIWKLQTREYVAIAHDWRSATRLRDRLGYVFGPPGWAPRTTDRVDAAVPVASS  311


>gi|240173100|ref|ZP_04751758.1| membrane-bound C-5 sterol desaturase Erg3 [Mycobacterium kansasii 
ATCC 12478]
Length=312

 Score =  520 bits (1338),  Expect = 2e-145, Method: Compositional matrix adjust.
 Identities = 247/285 (87%), Positives = 268/285 (95%), Gaps = 0/285 (0%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            +RDP LFAIP FL LL LEWTAARKLE IE AATG+PRPA+GAYLTRDSVASISMGLVSI
Sbjct  12   LRDPALFAIPFFLFLLALEWTAARKLERIEAAATGRPRPAAGAYLTRDSVASISMGLVSI  71

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
            ATTA WK LALLGYAAIYAY+APW LSA +WYTWVIAI GVDLLYY+YHRIAHRVRLIWA
Sbjct  72   ATTAAWKGLALLGYAAIYAYVAPWHLSAGQWYTWVIAIAGVDLLYYTYHRIAHRVRLIWA  131

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPL+G+PPWMVF ++S++LIYQFWVHTE
Sbjct  132  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLLGVPPWMVFFAFSVSLIYQFWVHTE  191

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            RI++LPRWFEF+FNTPSHHRVHHGMD +YLDKNYGGILIIWDRLFGSFQ E+FRPHYGLT
Sbjct  192  RINKLPRWFEFIFNTPSHHRVHHGMDQIYLDKNYGGILIIWDRLFGSFQAEVFRPHYGLT  251

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPR  285
            K+V+TFNIWKLQT EY+AI RD RSATRLRDRLGYVFGPPGW+PR
Sbjct  252  KQVNTFNIWKLQTYEYLAIARDCRSATRLRDRLGYVFGPPGWQPR  296


>gi|118466987|ref|YP_882088.1| C-5 sterol desaturase [Mycobacterium avium 104]
 gi|118168274|gb|ABK69171.1| C-5 sterol desaturase [Mycobacterium avium 104]
Length=308

 Score =  506 bits (1302),  Expect = 2e-141, Method: Compositional matrix adjust.
 Identities = 243/299 (82%), Positives = 267/299 (90%), Gaps = 6/299 (2%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            +RDPVL AIP FLLLL LEWTAARKLE +        RPA GA+ TRDS+ SISMGLVS+
Sbjct  15   LRDPVLVAIPFFLLLLTLEWTAARKLEHLTA------RPAPGAHRTRDSLTSISMGLVSV  68

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
            ATTAGWK+LAL GYAAIYAYLAPW L A RWYTW I I+GVDLLYY+YHRIAHRVRLIWA
Sbjct  69   ATTAGWKTLALFGYAAIYAYLAPWHLPATRWYTWAIPILGVDLLYYAYHRIAHRVRLIWA  128

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            THQAHHSSEY+NFATALRQKWNNSGEILMW+PLPL+G+PPWMVF S+SLNLIYQFW+HTE
Sbjct  129  THQAHHSSEYYNFATALRQKWNNSGEILMWIPLPLLGIPPWMVFFSFSLNLIYQFWIHTE  188

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            RID+LPR FEFVFNTPSHHRVHHGMD VYLDKNYGGILI+WDRLFG+FQ ELFRPHYGLT
Sbjct  189  RIDKLPRPFEFVFNTPSHHRVHHGMDKVYLDKNYGGILIVWDRLFGTFQAELFRPHYGLT  248

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTIDKSNAAASLVTS  299
            K+VDTFN+WKLQTREYVAI RDWRSA+RLRDRLGYVFGPPGW PR+  ++ A A +VTS
Sbjct  249  KQVDTFNVWKLQTREYVAIARDWRSASRLRDRLGYVFGPPGWAPRSAGRTAAGAPVVTS  307


>gi|183982698|ref|YP_001850989.1| membrane-bound C-5 sterol desaturase Erg3 [Mycobacterium marinum 
M]
 gi|183176024|gb|ACC41134.1| membrane-bound C-5 sterol desaturase Erg3 [Mycobacterium marinum 
M]
Length=302

 Score =  484 bits (1247),  Expect = 6e-135, Method: Compositional matrix adjust.
 Identities = 245/301 (82%), Positives = 266/301 (89%), Gaps = 2/301 (0%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            MRDPVLFAIP FL LL +EW AARKLE +E  AT  PRPASG+Y TRDSVASISMGLVS+
Sbjct  1    MRDPVLFAIPFFLFLLTVEWAAARKLEKLEAGATETPRPASGSYHTRDSVASISMGLVSL  60

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
             T A WK+LALLGYAAIY Y+APW LS  RWYTWVIAIVGVDLLYY+YHRIAHRVRLIWA
Sbjct  61   VTIAFWKTLALLGYAAIYTYVAPWHLSPGRWYTWVIAIVGVDLLYYTYHRIAHRVRLIWA  120

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            THQAHHSSEYFNFATALRQKWNNSGE+LMWVPLPL+GLPPWMVF  +S++LIYQFWVHTE
Sbjct  121  THQAHHSSEYFNFATALRQKWNNSGEVLMWVPLPLLGLPPWMVFFGFSVSLIYQFWVHTE  180

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            RI++LPRWFEF+FNTPSHHRVHHGMD +YLDKNYGGILIIWDRLF SFQ E  RPHYGLT
Sbjct  181  RINKLPRWFEFIFNTPSHHRVHHGMDHMYLDKNYGGILIIWDRLFRSFQAETARPHYGLT  240

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTID--KSNAAASLVT  298
            K VDTFNIWKLQTREYVAI RDWRSATRLRDRLGY FGPPGW+P T    ++  AA++ T
Sbjct  241  KPVDTFNIWKLQTREYVAIARDWRSATRLRDRLGYAFGPPGWQPSTTATPRAEDAAAVTT  300

Query  299  S  299
            S
Sbjct  301  S  301


>gi|118618451|ref|YP_906783.1| membrane-bound C-5 sterol desaturase Erg3 [Mycobacterium ulcerans 
Agy99]
 gi|118570561|gb|ABL05312.1| membrane-bound C-5 sterol desaturase Erg3 [Mycobacterium ulcerans 
Agy99]
Length=320

 Score =  481 bits (1239),  Expect = 5e-134, Method: Compositional matrix adjust.
 Identities = 242/301 (81%), Positives = 265/301 (89%), Gaps = 2/301 (0%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            MRDPVLFAIP FL LL +EW AARKLE +E  AT  PRP SG+Y TRDSVA+ISMGLVS+
Sbjct  15   MRDPVLFAIPFFLFLLTVEWAAARKLEKLEAGATETPRPVSGSYHTRDSVATISMGLVSL  74

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
             T A WK++ALLGYAAIY Y+APW LS  RWYTWVIAIVGVDLLYY+YHRIAHRVRLIWA
Sbjct  75   VTIAFWKTMALLGYAAIYTYVAPWHLSPGRWYTWVIAIVGVDLLYYTYHRIAHRVRLIWA  134

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            THQAHHSSEYFNFATALRQKWNNSGE+LMWVPLPL+GLPPWMVF  +S++LIYQFWVHTE
Sbjct  135  THQAHHSSEYFNFATALRQKWNNSGEVLMWVPLPLLGLPPWMVFFGFSVSLIYQFWVHTE  194

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            RI++LPRWFEF+FNTPSHHRVHHGMD +YLDKNYGGILIIWDRLF SFQ E  RPHYGLT
Sbjct  195  RINKLPRWFEFIFNTPSHHRVHHGMDQMYLDKNYGGILIIWDRLFRSFQAETARPHYGLT  254

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRT--IDKSNAAASLVT  298
            K VDTFNIWKLQTREYVAI RDWRSATRLRDRLGY FGPPGW+P T    ++  AA++ T
Sbjct  255  KPVDTFNIWKLQTREYVAIARDWRSATRLRDRLGYAFGPPGWQPSTTATPRAEDAATVTT  314

Query  299  S  299
            S
Sbjct  315  S  315


>gi|41407623|ref|NP_960459.1| hypothetical protein MAP1525 [Mycobacterium avium subsp. paratuberculosis 
K-10]
 gi|41395976|gb|AAS03842.1| hypothetical protein MAP_1525 [Mycobacterium avium subsp. paratuberculosis 
K-10]
Length=294

 Score =  479 bits (1234),  Expect = 2e-133, Method: Compositional matrix adjust.
 Identities = 242/299 (81%), Positives = 265/299 (89%), Gaps = 6/299 (2%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            MRDPVL AIP FLLLL LEWTAARKLE +        RPA GA+ TRDS+ SISMGLVS+
Sbjct  1    MRDPVLVAIPFFLLLLTLEWTAARKLEHLTA------RPAPGAHQTRDSLTSISMGLVSV  54

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
            ATTAGWK+LAL GYAAIYAYLAPW L A RWYTW IAI+GVDLLYY+YHRIAHRVRLIWA
Sbjct  55   ATTAGWKTLALFGYAAIYAYLAPWHLPATRWYTWAIAILGVDLLYYAYHRIAHRVRLIWA  114

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            THQAHHSSEY+NFATALRQKWNNSGEILMW+PLPL+G+PPWMVF S+S+NLIYQFW+HTE
Sbjct  115  THQAHHSSEYYNFATALRQKWNNSGEILMWLPLPLLGIPPWMVFFSFSVNLIYQFWIHTE  174

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            RID+LPR FEFVFNTPSHHRVHHGMD VYLDKNYGGILI+WDRLFG+FQ ELFRPHYGLT
Sbjct  175  RIDKLPRPFEFVFNTPSHHRVHHGMDKVYLDKNYGGILIVWDRLFGTFQAELFRPHYGLT  234

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTIDKSNAAASLVTS  299
            K VDTFN+W LQTRE VAI RDWRSA+RLRDRLGYVFGPPGW PR+  ++ A A +VTS
Sbjct  235  KHVDTFNVWTLQTRESVAIARDWRSASRLRDRLGYVFGPPGWAPRSAGRTAAGAPVVTS  293


>gi|336457575|gb|EGO36580.1| sterol desaturase [Mycobacterium avium subsp. paratuberculosis 
S397]
Length=308

 Score =  479 bits (1232),  Expect = 3e-133, Method: Compositional matrix adjust.
 Identities = 241/299 (81%), Positives = 265/299 (89%), Gaps = 6/299 (2%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            +RDPVL AIP FLLLL LEWTAARKLE +        RPA GA+ TRDS+ SISMGLVS+
Sbjct  15   LRDPVLVAIPFFLLLLTLEWTAARKLEHLTA------RPAPGAHQTRDSLTSISMGLVSV  68

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
            ATTAGWK+LAL GYAAIYAYLAPW L A RWYTW IAI+GVDLLYY+YHRIAHRVRLIWA
Sbjct  69   ATTAGWKTLALFGYAAIYAYLAPWHLPATRWYTWAIAILGVDLLYYAYHRIAHRVRLIWA  128

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            THQAHHSSEY+NFATALRQKWNNSGEILMW+PLPL+G+PPWMVF S+S+NLIYQFW+HTE
Sbjct  129  THQAHHSSEYYNFATALRQKWNNSGEILMWLPLPLLGIPPWMVFFSFSVNLIYQFWIHTE  188

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            RID+LPR FEFVFNTPSHHRVHHGMD VYLDKNYGGILI+WDRLFG+FQ ELFRPHYGLT
Sbjct  189  RIDKLPRPFEFVFNTPSHHRVHHGMDKVYLDKNYGGILIVWDRLFGTFQAELFRPHYGLT  248

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTIDKSNAAASLVTS  299
            K VDTFN+W LQTRE VAI RDWRSA+RLRDRLGYVFGPPGW PR+  ++ A A +VTS
Sbjct  249  KHVDTFNVWTLQTRESVAIARDWRSASRLRDRLGYVFGPPGWAPRSAGRTAAGAPVVTS  307


>gi|145224004|ref|YP_001134682.1| sterol desaturase-like protein [Mycobacterium gilvum PYR-GCK]
 gi|145216490|gb|ABP45894.1| sterol desaturase-related protein [Mycobacterium gilvum PYR-GCK]
Length=306

 Score =  459 bits (1181),  Expect = 2e-127, Method: Compositional matrix adjust.
 Identities = 223/295 (76%), Positives = 251/295 (86%), Gaps = 1/295 (0%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLE-SIETAATGQPRPASGAYLTRDSVASISMGLVS  59
            MR+PVLFAIP FLLLL +EWTAARKLE ++E  A     PA+GAY TRDS AS+SMGLVS
Sbjct  11   MREPVLFAIPFFLLLLAIEWTAARKLEHAVEGGADEDGTPAAGAYHTRDSWASLSMGLVS  70

Query  60   IATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIW  119
              T + WK  ALLGYAAIYAYLAPWQLS  +WYTWVIAIVGVDLL+Y YHR AHRVRL+W
Sbjct  71   TVTMSVWKFFALLGYAAIYAYLAPWQLSPTQWYTWVIAIVGVDLLFYVYHRTAHRVRLVW  130

Query  120  ATHQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHT  179
            ATHQAHHSS YFNFATALRQKWNNSGEILMW+PLPL+G+PPWMVF ++S++LIYQFW+HT
Sbjct  131  ATHQAHHSSRYFNFATALRQKWNNSGEILMWIPLPLLGVPPWMVFVAFSISLIYQFWIHT  190

Query  180  ERIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGL  239
            E I +L R FEFVFNTPSHHRVHHGMDP+YLDKNYGGILIIWDRLFG+FQPE+FRPHYGL
Sbjct  191  EHIGKLWRPFEFVFNTPSHHRVHHGMDPLYLDKNYGGILIIWDRLFGTFQPEVFRPHYGL  250

Query  240  TKRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTIDKSNAAA  294
            TK VDTFNIWKLQT EY AI RD R+A  +R +LGY+FGPPGW+P  +      A
Sbjct  251  TKPVDTFNIWKLQTHEYAAIWRDARAARGVRAKLGYIFGPPGWQPAQVQTPAPVA  305


>gi|315444336|ref|YP_004077215.1| sterol desaturase [Mycobacterium sp. Spyr1]
 gi|315262639|gb|ADT99380.1| sterol desaturase [Mycobacterium sp. Spyr1]
Length=306

 Score =  456 bits (1173),  Expect = 2e-126, Method: Compositional matrix adjust.
 Identities = 222/295 (76%), Positives = 250/295 (85%), Gaps = 1/295 (0%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLE-SIETAATGQPRPASGAYLTRDSVASISMGLVS  59
            MR+PVLFAIP FLLLL +EWTAARKLE ++E  A     PA+GAY TRDS AS+SMGLVS
Sbjct  11   MREPVLFAIPFFLLLLAIEWTAARKLEHAVEGGADEDGTPAAGAYHTRDSWASLSMGLVS  70

Query  60   IATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIW  119
              T + WK  ALLGYAAIYAYLAPWQLS  +WYTWVIAIVGVDLL+Y YHR AHRVRL+W
Sbjct  71   TVTMSVWKFFALLGYAAIYAYLAPWQLSPTQWYTWVIAIVGVDLLFYVYHRTAHRVRLVW  130

Query  120  ATHQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHT  179
            ATHQAHHSS YFNFATALRQKWNNSGEILMW+PLPL+G+PPWMVF ++S++LIYQFW+HT
Sbjct  131  ATHQAHHSSRYFNFATALRQKWNNSGEILMWIPLPLLGVPPWMVFVAFSISLIYQFWIHT  190

Query  180  ERIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGL  239
            E I +L R FEFVFNTPSHHRVHHGMD +YLDKNYGGILIIWDRLFG+FQPE+FRPHYGL
Sbjct  191  EHIGKLWRPFEFVFNTPSHHRVHHGMDQLYLDKNYGGILIIWDRLFGTFQPEVFRPHYGL  250

Query  240  TKRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTIDKSNAAA  294
            TK VDTFNIWKLQT EY AI RD R+A  +R +LGY+FGPPGW+P  +      A
Sbjct  251  TKPVDTFNIWKLQTHEYAAIWRDARAARGVRAKLGYIFGPPGWQPAQVQTPAPVA  305


>gi|333990550|ref|YP_004523164.1| membrane-bound C-5 sterol desaturase Erg3 [Mycobacterium sp. 
JDM601]
 gi|333486518|gb|AEF35910.1| membrane-bound C-5 sterol desaturase Erg3 [Mycobacterium sp. 
JDM601]
Length=299

 Score =  453 bits (1166),  Expect = 1e-125, Method: Compositional matrix adjust.
 Identities = 222/296 (75%), Positives = 249/296 (85%), Gaps = 13/296 (4%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            MRDPVLFAIP FLLLL LEWTAARKL  +E           G+Y  RD+  SISMGLVS+
Sbjct  15   MRDPVLFAIPFFLLLLTLEWTAARKLADLE----------RGSYRARDAWTSISMGLVSV  64

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
            ATTAGWK LALLGYAAIY YLAPW LS  +WYTWVIAI+GVDLLYY+YHRIAHRVRLIWA
Sbjct  65   ATTAGWKFLALLGYAAIYTYLAPWHLSPRQWYTWVIAILGVDLLYYTYHRIAHRVRLIWA  124

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            THQAHHSS+Y+N ATALRQKWNNSGE+L+W+PLPL G+PPWMVF ++S+NLIYQFWVHTE
Sbjct  125  THQAHHSSQYYNLATALRQKWNNSGEVLLWIPLPLFGIPPWMVFAAFSVNLIYQFWVHTE  184

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            RID+LPR  EF+FNTPSHHRVHHGMD  YLDKNYGGILI+WDRLFGSF PE FRPHYGLT
Sbjct  185  RIDKLPRVIEFIFNTPSHHRVHHGMDQEYLDKNYGGILIVWDRLFGSFAPERFRPHYGLT  244

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTIDKSNAAASL  296
            K+VDTFNI KLQ  EY +I+ D R+A   RDRLGYVFGPPGW+P +   S A+A+L
Sbjct  245  KQVDTFNIVKLQIHEYASIIADVRAARSWRDRLGYVFGPPGWQPAS---SRASAAL  297


>gi|120404150|ref|YP_953979.1| sterol desaturase--like protein [Mycobacterium vanbaalenii PYR-1]
 gi|119956968|gb|ABM13973.1| sterol desaturase-related protein [Mycobacterium vanbaalenii 
PYR-1]
Length=305

 Score =  452 bits (1164),  Expect = 2e-125, Method: Compositional matrix adjust.
 Identities = 222/286 (78%), Positives = 250/286 (88%), Gaps = 3/286 (1%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            MR+PVLFAIP FLLLL +EWTAARKLE   TA  G   PA GAY TRDS AS+SMGLVS 
Sbjct  11   MREPVLFAIPFFLLLLTIEWTAARKLE--HTAPDGG-SPAVGAYHTRDSWASVSMGLVST  67

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
             T + WK LALLGYAA+YAY+APWQLSA +WYTWVIA+VGVDLL+Y YHR+AHRVR++WA
Sbjct  68   VTMSVWKFLALLGYAALYAYVAPWQLSATQWYTWVIALVGVDLLFYVYHRMAHRVRIVWA  127

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            THQAHHSS YFNFATALRQKWNNSGEILMW+PLPL+G+PPWMVF ++S++LIYQFW+HTE
Sbjct  128  THQAHHSSRYFNFATALRQKWNNSGEILMWIPLPLLGVPPWMVFFAFSVSLIYQFWIHTE  187

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            RI +L    EF+FNTPSHHRVHHGMD +YLDKNYGGILIIWDRLFG+FQPE+FRPHYGLT
Sbjct  188  RIGKLWLPVEFIFNTPSHHRVHHGMDKLYLDKNYGGILIIWDRLFGTFQPEVFRPHYGLT  247

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRT  286
            K VDTFNIWKLQT EYVAI RD R+A  LR +LGY+FGPPGWEP T
Sbjct  248  KPVDTFNIWKLQTHEYVAIGRDVRAARGLRAKLGYIFGPPGWEPST  293


>gi|118470601|ref|YP_887969.1| C-5 sterol desaturase [Mycobacterium smegmatis str. MC2 155]
 gi|118171888|gb|ABK72784.1| C-5 sterol desaturase [Mycobacterium smegmatis str. MC2 155]
Length=307

 Score =  449 bits (1155),  Expect = 3e-124, Method: Compositional matrix adjust.
 Identities = 217/292 (75%), Positives = 251/292 (86%), Gaps = 3/292 (1%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            MRDPV+FAIP FLLLL +EWTAAR+LE ++ A     R  +GA+ TRD+  S+SMGLVS+
Sbjct  15   MRDPVMFAIPFFLLLLTIEWTAARRLEHVDPAEGD--RAPAGAFHTRDAWTSVSMGLVSV  72

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
            ATTAGWK LALLGYA +YAY+APWQL A +WYTWVIA++GVDLL+Y+YHR+AHRVRLIWA
Sbjct  73   ATTAGWKLLALLGYAVLYAYVAPWQLPADKWYTWVIALLGVDLLWYTYHRVAHRVRLIWA  132

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            THQAHHSSEYFNFATALRQKWNNSGEI+ W+PLPL+G+PPW+VF ++S+NLIYQFWVHTE
Sbjct  133  THQAHHSSEYFNFATALRQKWNNSGEIVAWIPLPLLGIPPWVVFAAFSVNLIYQFWVHTE  192

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            RI +LPR  EFVFNTPSHHRVHHG D +YLD+NYGGILIIWDRLFG+FQ E+FRP YGLT
Sbjct  193  RIGKLPRPIEFVFNTPSHHRVHHGSDELYLDRNYGGILIIWDRLFGTFQEEVFRPRYGLT  252

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEP-RTIDKSN  291
            K V T+NIWKLQT EYVAI  D RSA R  DRLGYVFGPPGW P RT + + 
Sbjct  253  KPVGTYNIWKLQTHEYVAIAHDVRSARRWTDRLGYVFGPPGWTPARTCETTE  304


>gi|183984597|ref|YP_001852888.1| membrane-bound C-5 sterol desaturase Erg3_1 [Mycobacterium marinum 
M]
 gi|183177923|gb|ACC43033.1| membrane-bound C-5 sterol desaturase Erg3_1 [Mycobacterium marinum 
M]
Length=313

 Score =  430 bits (1106),  Expect = 1e-118, Method: Compositional matrix adjust.
 Identities = 198/286 (70%), Positives = 237/286 (83%), Gaps = 0/286 (0%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            ++DPV+   P  +LL++LEW AARKL +    A    R A GA+   D++AS+S GLVS+
Sbjct  15   LQDPVVLTAPFLILLIVLEWIAARKLLTTSAPAADDSRNAPGAHFGPDTIASLSTGLVSL  74

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
             T A WK++A +GYAAIY Y+APW LS H+WYTWVIA++G+DL+Y   HRIAHRVRLIWA
Sbjct  75   VTGATWKTIAAIGYAAIYTYVAPWHLSPHQWYTWVIAVLGLDLIYCVDHRIAHRVRLIWA  134

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
             HQ HHSSEYFN ATA+R +WN SGEI+M+  LPL+G+PPW+VF SWS+NL YQFWVHTE
Sbjct  135  AHQPHHSSEYFNLATAVRVEWNKSGEIIMFAILPLLGVPPWVVFFSWSINLTYQFWVHTE  194

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            RI +LPRW+E++FNTPSHHRVHHGMD +YLDKN+GGILIIWDR+ G+FQ E FRPHYGLT
Sbjct  195  RIGKLPRWYEYLFNTPSHHRVHHGMDQMYLDKNFGGILIIWDRMLGTFQAEEFRPHYGLT  254

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRT  286
            K V TFNIWKLQT EY AIVRDWRSATRLRDRLGYVFGPPGW+P+T
Sbjct  255  KPVHTFNIWKLQTNEYAAIVRDWRSATRLRDRLGYVFGPPGWKPQT  300


>gi|108799830|ref|YP_640027.1| sterol desaturase-like protein [Mycobacterium sp. MCS]
 gi|119868940|ref|YP_938892.1| sterol desaturase-like protein [Mycobacterium sp. KMS]
 gi|108770249|gb|ABG08971.1| sterol desaturase-related protein [Mycobacterium sp. MCS]
 gi|119695029|gb|ABL92102.1| sterol desaturase-related protein [Mycobacterium sp. KMS]
Length=312

 Score =  428 bits (1100),  Expect = 6e-118, Method: Compositional matrix adjust.
 Identities = 214/284 (76%), Positives = 248/284 (88%), Gaps = 4/284 (1%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            +R+PVLFA+P FLLLLILEWTAARKLE +E+      +P +GAY+ RD+  S++MGLVS+
Sbjct  19   LREPVLFAVPFFLLLLILEWTAARKLEHLESG----DQPPAGAYVARDAWTSLAMGLVSV  74

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
             T A WK  AL+GYAA+YAY+APWQL +++WYTWVIAIVGVDLL+YSYHRIAHRVRL+WA
Sbjct  75   GTMAVWKFGALIGYAALYAYVAPWQLPSNQWYTWVIAIVGVDLLFYSYHRIAHRVRLVWA  134

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            THQAHHSSEY+NFATALRQKWNNSGEI+MW+PLPL+G+PPWMVF  +S++LIYQFWVHTE
Sbjct  135  THQAHHSSEYYNFATALRQKWNNSGEIVMWLPLPLLGVPPWMVFAGFSISLIYQFWVHTE  194

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            RI  + R  EFVFNTPSHHRVHHG DP YLDKNYGGILIIWDRLFG+FQPELFRPHYGLT
Sbjct  195  RIGTMWRPIEFVFNTPSHHRVHHGRDPEYLDKNYGGILIIWDRLFGTFQPELFRPHYGLT  254

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEP  284
            K V+T+NIW+LQT EY AI RD R A R RDRLGY FGPPGW+P
Sbjct  255  KPVNTYNIWRLQTHEYAAIARDVRGARRWRDRLGYAFGPPGWKP  298


>gi|126435473|ref|YP_001071164.1| sterol desaturase-like protein [Mycobacterium sp. JLS]
 gi|126235273|gb|ABN98673.1| sterol desaturase-related protein [Mycobacterium sp. JLS]
Length=312

 Score =  427 bits (1098),  Expect = 1e-117, Method: Compositional matrix adjust.
 Identities = 213/284 (75%), Positives = 248/284 (88%), Gaps = 4/284 (1%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            +R+PVLFA+P FLLLLILEWTAARKLE +E+      +P +GAY+ RD+  S++MGLVS+
Sbjct  19   LREPVLFAVPFFLLLLILEWTAARKLEHLESG----DQPPAGAYVARDAWTSLAMGLVSV  74

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
             T A WK  AL+GYAA+YAY+APWQL +++WYTWVIAIVGVDLL+YSYHRIAHRVRL+WA
Sbjct  75   GTMAVWKFGALIGYAALYAYVAPWQLPSNQWYTWVIAIVGVDLLFYSYHRIAHRVRLVWA  134

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            THQAHHSSEY+NFATA+RQKWNNSGEI+MW+PLPL+G+PPWMVF  +S++LIYQFWVHTE
Sbjct  135  THQAHHSSEYYNFATAVRQKWNNSGEIVMWLPLPLLGVPPWMVFAGFSISLIYQFWVHTE  194

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            RI  + R  EFVFNTPSHHRVHHG DP YLDKNYGGILIIWDRLFG+FQPELFRPHYGLT
Sbjct  195  RIGTMWRPIEFVFNTPSHHRVHHGRDPEYLDKNYGGILIIWDRLFGTFQPELFRPHYGLT  254

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEP  284
            K V+T+NIW+LQT EY AI RD R A R RDRLGY FGPPGW+P
Sbjct  255  KPVNTYNIWRLQTHEYAAIARDVRGARRWRDRLGYAFGPPGWKP  298


>gi|226363499|ref|YP_002781281.1| hypothetical protein ROP_40890 [Rhodococcus opacus B4]
 gi|226241988|dbj|BAH52336.1| hypothetical membrane protein [Rhodococcus opacus B4]
Length=322

 Score =  422 bits (1085),  Expect = 3e-116, Method: Compositional matrix adjust.
 Identities = 197/291 (68%), Positives = 233/291 (81%), Gaps = 0/291 (0%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            + +PV  AIP F+L L +EW AAR LE   T   G+ RP  G Y  RD+ AS++MG VSI
Sbjct  17   LHEPVTLAIPAFVLFLAIEWFAARHLEDAATDPDGRSRPVRGGYERRDARASVAMGAVSI  76

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
             TT  WK LAL+GY+AI+ YLAPW L A  WYTWVI I+GVD+L+Y+YHR++HR+RLIWA
Sbjct  77   LTTTFWKLLALIGYSAIWVYLAPWHLPADAWYTWVILILGVDVLFYAYHRMSHRIRLIWA  136

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            THQAHHSSEYFNF+TALRQKWNNS EI MW+PLPL+G+PPWMVF  +S+NL+YQF+VHTE
Sbjct  137  THQAHHSSEYFNFSTALRQKWNNSAEIFMWIPLPLIGIPPWMVFIGFSVNLVYQFFVHTE  196

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            RID+LPR  EFVFNTPSHHRVHHG DP YLD+NY GILI+WDR+FG+FQPE  RP YGLT
Sbjct  197  RIDKLPRPVEFVFNTPSHHRVHHGCDPEYLDRNYAGILIVWDRMFGTFQPETHRPTYGLT  256

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTIDKSN  291
            K V T+NIW LQT EY AI RDWR A+  RDRLGYVFGPPGW P    +++
Sbjct  257  KPVGTYNIWTLQTHEYAAIGRDWRRASSWRDRLGYVFGPPGWTPSGTREAD  307


>gi|312137812|ref|YP_004005148.1| integral membrane protein [Rhodococcus equi 103S]
 gi|311887151|emb|CBH46460.1| putative integral membrane protein [Rhodococcus equi 103S]
Length=320

 Score =  421 bits (1081),  Expect = 1e-115, Method: Compositional matrix adjust.
 Identities = 201/291 (70%), Positives = 239/291 (83%), Gaps = 1/291 (0%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            + DPV  AIP F++ L LEW AARKLE  E    G+  P +  Y  RD+ ASISMGLVSI
Sbjct  16   LGDPVTLAIPAFVVFLGLEWFAARKLEETEIR-DGRAYPPTAGYEFRDARASISMGLVSI  74

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
            ATTA WK LAL+GY+AI+ YLAPW L A+ WYTWV+ +VG+DLLYY+YHR+AHR+RLIWA
Sbjct  75   ATTAFWKFLALIGYSAIWVYLAPWHLPANAWYTWVLLLVGIDLLYYTYHRMAHRIRLIWA  134

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            THQAHHSSEYFNFATALRQKWNNSGEILMW+PLPL+G+PPWM+F  +S++L+YQF+VHTE
Sbjct  135  THQAHHSSEYFNFATALRQKWNNSGEILMWIPLPLIGIPPWMIFVGFSVSLVYQFFVHTE  194

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            R+DRLPR  E+VFNTPSHHRVHHG DP YLD+NYGGILI+WDR+FG+F+ E  RP YGLT
Sbjct  195  RVDRLPRPIEYVFNTPSHHRVHHGCDPEYLDRNYGGILIVWDRMFGTFRAEQQRPTYGLT  254

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTIDKSN  291
            K V T++IWKLQT EY AI RD R+A   RDRLGYVFGPPGW P    +++
Sbjct  255  KPVGTYDIWKLQTHEYAAIGRDIRAAQSWRDRLGYVFGPPGWAPSGAGQAH  305


>gi|325676085|ref|ZP_08155767.1| C-5 sterol desaturase [Rhodococcus equi ATCC 33707]
 gi|325553125|gb|EGD22805.1| C-5 sterol desaturase [Rhodococcus equi ATCC 33707]
Length=320

 Score =  419 bits (1077),  Expect = 3e-115, Method: Compositional matrix adjust.
 Identities = 200/291 (69%), Positives = 239/291 (83%), Gaps = 1/291 (0%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            + DPV  AIP F++ L LEW AARKLE  E    G+  P +  Y  RD+ ASI MGLVSI
Sbjct  16   LGDPVTLAIPAFVVFLGLEWFAARKLEETEIR-DGRAYPPTAGYEFRDARASILMGLVSI  74

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
            ATTA WK LAL+GY+AI+ YLAPW L A+ WYTWV+ +VG+DLLYY+YHR+AHR+RLIWA
Sbjct  75   ATTAFWKFLALIGYSAIWVYLAPWHLPANAWYTWVLLLVGIDLLYYTYHRMAHRIRLIWA  134

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            THQAHHSSEYFNFATALRQKWNNSGEILMW+PLPL+G+PPWM+F  +S++L+YQF+VHTE
Sbjct  135  THQAHHSSEYFNFATALRQKWNNSGEILMWIPLPLIGIPPWMIFVGFSVSLVYQFFVHTE  194

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            R+DRLPR  E+VFNTPSHHRVHHG DP YLD+NYGGILI+WDR+FG+F+ E  RP YGLT
Sbjct  195  RVDRLPRPIEYVFNTPSHHRVHHGCDPEYLDRNYGGILIVWDRMFGTFRAEQQRPTYGLT  254

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTIDKSN  291
            K V T++IWKLQT EY AI RD R+A   RDRLGYVFGPPGW P +  +++
Sbjct  255  KPVGTYDIWKLQTHEYAAIGRDVRAAQSWRDRLGYVFGPPGWAPSSAGQAH  305


>gi|229492567|ref|ZP_04386370.1| C-5 sterol desaturase [Rhodococcus erythropolis SK121]
 gi|229320553|gb|EEN86371.1| C-5 sterol desaturase [Rhodococcus erythropolis SK121]
Length=313

 Score =  405 bits (1040),  Expect = 5e-111, Method: Compositional matrix adjust.
 Identities = 188/285 (66%), Positives = 228/285 (80%), Gaps = 0/285 (0%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            + DPV  AIP F++ L +EW AAR LE     A G+ RPA G Y + D+  S+ +GLVSI
Sbjct  16   LHDPVTLAIPAFVIFLAIEWFAARALEDHPDDADGRARPAQGGYRSVDARTSVLLGLVSI  75

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
            AT+  WK LAL+GY+AI+ YLAPW LS   WYTWVI ++G+D+LYY+YHRIAHRVR+IWA
Sbjct  76   ATSTFWKVLALIGYSAIWVYLAPWHLSPTAWYTWVILLLGIDVLYYAYHRIAHRVRVIWA  135

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            THQAHHSSEYFNF+TALRQKWNNSGEI+MW+PLP +G+PPWMVF  +S++L+YQF+VHTE
Sbjct  136  THQAHHSSEYFNFSTALRQKWNNSGEIIMWLPLPFLGIPPWMVFVGFSVSLVYQFFVHTE  195

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            R+ +L R  EF+FNTPSHHRVHHG D  YLD+NY GILIIWDR+FG+F  E  RP YGLT
Sbjct  196  RVGKLWRPIEFIFNTPSHHRVHHGSDAEYLDRNYAGILIIWDRMFGTFAAETHRPTYGLT  255

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPR  285
              V T+NIWKLQTREY AI RDW+++T  R R GYVFGPPGW P+
Sbjct  256  TPVGTYNIWKLQTREYAAIARDWKASTSWRQRAGYVFGPPGWAPK  300


>gi|326383892|ref|ZP_08205576.1| fatty acid hydroxylase [Gordonia neofelifaecis NRRL B-59395]
 gi|326197351|gb|EGD54541.1| fatty acid hydroxylase [Gordonia neofelifaecis NRRL B-59395]
Length=298

 Score =  404 bits (1038),  Expect = 9e-111, Method: Compositional matrix adjust.
 Identities = 196/284 (70%), Positives = 225/284 (80%), Gaps = 3/284 (1%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            MR+P + AIP F+LLL LE  AA KLE  E    G  RPA GAY  RD+ ASI MG+VSI
Sbjct  11   MREPTVLAIPFFVLLLALETYAASKLEHDEN---GSGRPAKGAYDGRDARASIWMGVVSI  67

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
             T+A WK  AL GY A++AY+APWQ+    WYTWVI  V +D L+Y  HRIAHRVRL+WA
Sbjct  68   GTSAAWKFAALFGYTALFAYVAPWQMPVDAWYTWVIGFVALDFLFYWEHRIAHRVRLVWA  127

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            THQAHHSSEYFNFATALRQKWNNS  I+MW PLPL+G PP +VF  +SLNLIYQFW+HTE
Sbjct  128  THQAHHSSEYFNFATALRQKWNNSMGIIMWAPLPLLGFPPAVVFAVYSLNLIYQFWIHTE  187

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            RID++ R FEFV NTPSHHRVHHG DP YLD+NYGG  I+WDRLFGSFQPEL RP+YGLT
Sbjct  188  RIDKMWRPFEFVMNTPSHHRVHHGSDPEYLDRNYGGTFIVWDRLFGSFQPELARPNYGLT  247

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEP  284
            K V T+NIWKLQT EYV+I RD RSA+ +R +LGY+FGPPGW P
Sbjct  248  KPVGTYNIWKLQTHEYVSIGRDVRSASSIRAKLGYIFGPPGWAP  291


>gi|226303825|ref|YP_002763783.1| hypothetical protein RER_03360 [Rhodococcus erythropolis PR4]
 gi|226182940|dbj|BAH31044.1| conserved hypothetical membrane protein [Rhodococcus erythropolis 
PR4]
Length=313

 Score =  403 bits (1035),  Expect = 2e-110, Method: Compositional matrix adjust.
 Identities = 187/285 (66%), Positives = 227/285 (80%), Gaps = 0/285 (0%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            + DPV  AIP F++ L +EW AAR LE     A G+ RPA G Y + D+  S+ +GLVSI
Sbjct  16   LHDPVTLAIPAFVIFLAIEWFAARALEDHPDDADGRARPAEGGYRSVDARTSVLLGLVSI  75

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
            AT+  WK LAL+GY+AI+ YLAPW LS   WYTWVI ++G+D+LYY+YHRIAHRVR+IWA
Sbjct  76   ATSTFWKVLALIGYSAIWVYLAPWHLSPTAWYTWVILLLGIDVLYYAYHRIAHRVRVIWA  135

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            THQAHHSSEYFNF+TALRQKWNNSGEI+MW+PLP +G+PPWMVF  +S++L+YQF+VHTE
Sbjct  136  THQAHHSSEYFNFSTALRQKWNNSGEIIMWLPLPFLGIPPWMVFVGFSVSLVYQFFVHTE  195

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            R+ +L R  EF+FNTPSHHRVHHG D  YLD+NY GILIIWDR+FG+F  E  RP YGLT
Sbjct  196  RVGKLWRPIEFIFNTPSHHRVHHGSDAEYLDRNYAGILIIWDRMFGTFAAETHRPTYGLT  255

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPR  285
              V T+NIWKLQTREY AI RDW+++   R R GYVFGPPGW P+
Sbjct  256  TPVGTYNIWKLQTREYAAIARDWKASKSWRQRAGYVFGPPGWAPK  300


>gi|343928716|ref|ZP_08768161.1| hypothetical protein GOALK_120_01440 [Gordonia alkanivorans NBRC 
16433]
 gi|343761465|dbj|GAA15087.1| hypothetical protein GOALK_120_01440 [Gordonia alkanivorans NBRC 
16433]
Length=309

 Score =  371 bits (953),  Expect = 7e-101, Method: Compositional matrix adjust.
 Identities = 186/285 (66%), Positives = 226/285 (80%), Gaps = 1/285 (0%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLE-SIETAATGQPRPASGAYLTRDSVASISMGLVS  59
            MR+P + AIP F+ +L +EW AA KLE  +E    G+  P  GAY TRD+ AS+ MGLVS
Sbjct  9    MREPTVLAIPFFVTMLAIEWFAAAKLEHDLEDGLDGEGGPLPGAYETRDARASVLMGLVS  68

Query  60   IATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIW  119
            I T+A WK L LL Y A++ Y+APW L   +WY+WVIA V VD +YY  HR++HRVR++W
Sbjct  69   IGTSAFWKLLGLLAYTALFTYVAPWHLPMDQWYSWVIAFVAVDFIYYWVHRVSHRVRIVW  128

Query  120  ATHQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHT  179
            ATHQAHHSSEYFNFATALRQKWN S  I+MW+PLPL+G+PP +VF ++SLNLIYQFW+HT
Sbjct  129  ATHQAHHSSEYFNFATALRQKWNISAAIVMWLPLPLLGVPPALVFFAYSLNLIYQFWIHT  188

Query  180  ERIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGL  239
            E IDR+ R FE+VFNTPSHHRVHHG DP YLD+NYGG+ IIWDR+FGSF PE+ RP YGL
Sbjct  189  EHIDRMWRPFEYVFNTPSHHRVHHGRDPEYLDRNYGGVFIIWDRMFGSFTPEVRRPRYGL  248

Query  240  TKRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEP  284
            TK VDT+NIW+L+T EYVAI RD +S +  R RLGY+FGPPGW P
Sbjct  249  TKPVDTYNIWRLETHEYVAIARDVKSTSSWRARLGYIFGPPGWTP  293


>gi|111021138|ref|YP_704110.1| C-5 sterol desaturase [Rhodococcus jostii RHA1]
 gi|110820668|gb|ABG95952.1| probable C-5 sterol desaturase [Rhodococcus jostii RHA1]
Length=252

 Score =  371 bits (952),  Expect = 9e-101, Method: Compositional matrix adjust.
 Identities = 172/237 (73%), Positives = 201/237 (85%), Gaps = 0/237 (0%)

Query  55   MGLVSIATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHR  114
            MG VSI TT  WK LAL+GY+AI+ Y+APW L A  WYTWVI I+GVD L+Y+YHR+AHR
Sbjct  1    MGAVSILTTTFWKLLALIGYSAIWVYVAPWHLPADAWYTWVILILGVDALFYAYHRMAHR  60

Query  115  VRLIWATHQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQ  174
            +RLIWATHQAHHSSEYFNF+TALRQKWNNS EILMW+PLPL+G+PPWMVF  +S+NL+YQ
Sbjct  61   IRLIWATHQAHHSSEYFNFSTALRQKWNNSAEILMWIPLPLIGIPPWMVFIGFSVNLVYQ  120

Query  175  FWVHTERIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFR  234
            F+VHTERID+L R  EFVFNTPSHHRVHHG DP YLD+NY GILI+WDR+FG+FQPE  R
Sbjct  121  FFVHTERIDKLARPVEFVFNTPSHHRVHHGCDPEYLDRNYAGILIVWDRMFGTFQPETHR  180

Query  235  PHYGLTKRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTIDKSN  291
            P YGLT  V T++IWKLQTREY AIVRDWR A+  RD+LGYVFGPPGW P    +++
Sbjct  181  PTYGLTTPVGTYSIWKLQTREYGAIVRDWRRASSSRDKLGYVFGPPGWAPSGTREAD  237


>gi|262204643|ref|YP_003275851.1| fatty acid hydroxylase [Gordonia bronchialis DSM 43247]
 gi|262087990|gb|ACY23958.1| fatty acid hydroxylase [Gordonia bronchialis DSM 43247]
Length=323

 Score =  357 bits (915),  Expect = 2e-96, Method: Compositional matrix adjust.
 Identities = 184/286 (65%), Positives = 222/286 (78%), Gaps = 4/286 (1%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQP--RPAS--GAYLTRDSVASISMG  56
            MR+P + AIP F+++L LEW AA KLE  ET    Q   R +S  G Y  RDS AS+SMG
Sbjct  9    MREPTVLAIPFFVVMLTLEWLAAWKLEHTETERPSQREVRASSEPGTYNWRDSRASLSMG  68

Query  57   LVSIATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVR  116
            LVSIAT+AGWK L LL Y A++AY+APW L A  W+TW+IA V +D L+Y  HR+AHRVR
Sbjct  69   LVSIATSAGWKLLGLLAYTALFAYVAPWHLPAGAWWTWLIAFVALDFLFYWEHRLAHRVR  128

Query  117  LIWATHQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFW  176
            L+WATHQAHHSSEYFNF TALRQKWNNS  ++MW+PLPL+G+PP +VF  +S NL+YQFW
Sbjct  129  LVWATHQAHHSSEYFNFTTALRQKWNNSAGLVMWLPLPLLGVPPAVVFAVYSANLVYQFW  188

Query  177  VHTERIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPH  236
            +HTER+ +L    EFVFNTPSHHRVHHG D  YLD+NYGG  I+WDR+FG+F  E  RP 
Sbjct  189  IHTERVGKLWAPIEFVFNTPSHHRVHHGSDTEYLDRNYGGTFIVWDRMFGTFTAEAKRPT  248

Query  237  YGLTKRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGW  282
            YGLTK VDT+NIW+LQT EYVAI RD R+A   R+R+GY+FGPPGW
Sbjct  249  YGLTKPVDTYNIWRLQTHEYVAIARDVRAARGFRNRMGYLFGPPGW  294


>gi|319949067|ref|ZP_08023163.1| hypothetical membrane protein [Dietzia cinnamea P4]
 gi|319437286|gb|EFV92310.1| hypothetical membrane protein [Dietzia cinnamea P4]
Length=312

 Score =  327 bits (839),  Expect = 1e-87, Method: Compositional matrix adjust.
 Identities = 177/283 (63%), Positives = 212/283 (75%), Gaps = 10/283 (3%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            + +PVL AIP F+L + +EW +AR LE+ E           G Y  RD+V S+ MG  S 
Sbjct  18   LHEPVLLAIPVFVLFVAIEWVSARVLEARE----------PGGYQPRDAVTSLIMGASSQ  67

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
            A     K + L+ YAA++ YLAPW L    WYTWV+AI+G D LYY YHR AHR+RL+WA
Sbjct  68   AFWLLGKLVGLVLYAAVWVYLAPWHLPPDAWYTWVLAILGTDFLYYWYHRGAHRIRLVWA  127

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            THQAHHSSEY+NFATALRQKWNNSGEI+ W+PLPL+G+PPWMVF  +S++L+YQF+VHTE
Sbjct  128  THQAHHSSEYYNFATALRQKWNNSGEIIAWLPLPLLGVPPWMVFTGFSVSLVYQFFVHTE  187

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            RI RLP   EFVFNTPSHHRVHHG D  YLD+NYGGILIIWDRLFG+F+PE  RP YGLT
Sbjct  188  RIGRLPAPIEFVFNTPSHHRVHHGSDTEYLDRNYGGILIIWDRLFGTFRPETHRPTYGLT  247

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWE  283
              V++ N + LQ  EYVAI RD R A   R+RLGYVFGPPGW+
Sbjct  248  TPVNSHNPFWLQLHEYVAIWRDLRGAGSWRERLGYVFGPPGWK  290


>gi|311743305|ref|ZP_07717112.1| C-5 sterol desaturase [Aeromicrobium marinum DSM 15272]
 gi|311313373|gb|EFQ83283.1| C-5 sterol desaturase [Aeromicrobium marinum DSM 15272]
Length=289

 Score =  315 bits (808),  Expect = 4e-84, Method: Compositional matrix adjust.
 Identities = 151/290 (53%), Positives = 198/290 (69%), Gaps = 9/290 (3%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            +++PV +A P FLL +++E  A + L+  +             Y  +D+  SISMG+ S+
Sbjct  8    LKNPVTWAAPFFLLTILIELAALKWLDHDDNVT---------GYALKDARTSISMGIGSV  58

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
                 +K L+  G+ A+++YLA W+L    W+ WV+  +G+DL YY +HR +HRVR+ WA
Sbjct  59   FFLTVFKVLSFFGFVAVFSYLALWELPTDTWWYWVVLFLGLDLGYYFHHRFSHRVRIGWA  118

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
             HQAHHSSEY NF TALRQKWN   E   W+PLPL+G  PW ++ ++  NLIYQF+VHTE
Sbjct  119  GHQAHHSSEYMNFGTALRQKWNPWFEFFFWLPLPLLGFAPWTLYVAFGFNLIYQFFVHTE  178

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
             +DRLPR  E V NTPSHHRVHHG DP YLDKNY GI IIWDR+FG+F  E  RP YGLT
Sbjct  179  LVDRLPRPVELVMNTPSHHRVHHGSDPEYLDKNYAGIFIIWDRIFGTFAAEKQRPTYGLT  238

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTIDKS  290
             +VDT+N+ +LQ  EYV I RD R A  LR +LGYVFGPPGW+P+ + + 
Sbjct  239  YKVDTYNLMRLQYGEYVRIARDVRGAKSLRTKLGYVFGPPGWQPQEVPQK  288


>gi|291454322|ref|ZP_06593712.1| C-5 sterol desaturase [Streptomyces albus J1074]
 gi|291357271|gb|EFE84173.1| C-5 sterol desaturase [Streptomyces albus J1074]
Length=306

 Score =  313 bits (801),  Expect = 3e-83, Method: Compositional matrix adjust.
 Identities = 152/302 (51%), Positives = 200/302 (67%), Gaps = 3/302 (0%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASG---AYLTRDSVASISMGL  57
            +  P+L+A P F L   +E  +   L+  E    G  RP       +   DS  ++ MG+
Sbjct  2    INTPLLYATPFFALFAGIELASFALLDRAERREPGAERPPGDRPKGHSWPDSRTNLVMGM  61

Query  58   VSIATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRL  117
             S+ +TA  K+  L+ +  +YA LAPW L    W++WV+ ++ VDL +Y+ HR +HRVR+
Sbjct  62   GSLVSTAVLKAATLVLFVYLYAALAPWHLPTDTWWSWVLLLIAVDLAWYTNHRFSHRVRI  121

Query  118  IWATHQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWV  177
             WA HQAHHSSEYFN  TA+RQKWN   E + W PLPL+G  PW ++ ++S NL+YQF+ 
Sbjct  122  GWAGHQAHHSSEYFNLTTAVRQKWNPWSEAIFWAPLPLLGFAPWTIYVAFSFNLVYQFFT  181

Query  178  HTERIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHY  237
            HTE + +LPR  EFV NTPSHHRVHH  DP YLDKNYGGILI+WDRLFG+FQ E  RP Y
Sbjct  182  HTEAVGKLPRPVEFVLNTPSHHRVHHASDPEYLDKNYGGILIVWDRLFGTFQEETHRPTY  241

Query  238  GLTKRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTIDKSNAAASLV  297
            GLTK V+T+N++ LQ  EY  I+ D RSA RLRD+LGYVF PPGW+P   D +    +L 
Sbjct  242  GLTKPVNTYNVFTLQYHEYGNILADVRSAPRLRDKLGYVFRPPGWKPTPPDGAGDGGALA  301

Query  298  TS  299
             +
Sbjct  302  PA  303


>gi|300784689|ref|YP_003764980.1| sterol desaturase [Amycolatopsis mediterranei U32]
 gi|299794203|gb|ADJ44578.1| sterol desaturase [Amycolatopsis mediterranei U32]
 gi|340526112|gb|AEK41317.1| sterol desaturase [Amycolatopsis mediterranei S699]
Length=286

 Score =  308 bits (789),  Expect = 7e-82, Method: Compositional matrix adjust.
 Identities = 147/287 (52%), Positives = 201/287 (71%), Gaps = 10/287 (3%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            +RDPVLFAIP FLL + +E  A   L   +             Y   D+  S+ MG V++
Sbjct  8    LRDPVLFAIPVFLLFVTIEIVAVHVLGHDDGIV---------GYSVADTRTSMLMGTVAV  58

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
               A ++ + L+ +AA++  LAP +L    W+ WV+ ++G +L++Y+YHR +HRVRL+WA
Sbjct  59   GVNALFRLVMLVVFAALFE-LAPVKLDPRDWWAWVLMLLGQELVFYAYHRASHRVRLLWA  117

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
             HQ HHSSE++NF+TALRQKW    ++  W  L L G+PPWM+    S++L+YQF+VHTE
Sbjct  118  GHQVHHSSEHYNFSTALRQKWTPYFQLPFWSVLALCGIPPWMILTGLSIDLVYQFFVHTE  177

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            ++ +LPRWFE+VFNTPSHHRVHHG DP YLD NYGGILI+WDRLFGSF PE  RP YGLT
Sbjct  178  KVGKLPRWFEYVFNTPSHHRVHHGSDPEYLDANYGGILIVWDRLFGSFVPEGRRPTYGLT  237

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTI  287
            K ++T+++ K+   EY +I+RD R+A   R+R GYVFGPPGW+P  +
Sbjct  238  KNIETYHLLKVGFHEYGSILRDLRTARTWRERAGYVFGPPGWQPEHV  284


>gi|84494268|ref|ZP_00993387.1| hypothetical protein JNB_05719 [Janibacter sp. HTCC2649]
 gi|84383761|gb|EAP99641.1| hypothetical protein JNB_05719 [Janibacter sp. HTCC2649]
Length=305

 Score =  296 bits (758),  Expect = 3e-78, Method: Compositional matrix adjust.
 Identities = 148/284 (53%), Positives = 193/284 (68%), Gaps = 4/284 (1%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
              DPV FAIP F L ++LE  A + L+  +      P+     Y + D+ +++   + S+
Sbjct  12   FHDPVTFAIPFFALFIVLELLALKYLDDHDDELGENPK---KGYSSPDTASNLGSAVGSV  68

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
                G +  AL  Y A++  +APW + A   +TWV  ++ VDLL+YSYHR +HR+R++WA
Sbjct  69   IINLGARFAALFLYFALWN-IAPWHMDASNPWTWVFTLLAVDLLWYSYHRASHRIRILWA  127

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
             HQAHHSS YFN++ ALRQKWN  GE+L W PLPL+G+PPWM+F ++SLNLIYQFWVHTE
Sbjct  128  AHQAHHSSVYFNYSVALRQKWNPWGELLFWTPLPLLGVPPWMIFFAFSLNLIYQFWVHTE  187

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
             I +L   FEF+FNTPSHHRVHH     YLDKNY GILIIWDR+FG++  E   P YGLT
Sbjct  188  TIPKLWGPFEFIFNTPSHHRVHHASQKQYLDKNYAGILIIWDRIFGTYAEEEDIPVYGLT  247

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEP  284
              V T N ++LQ  E++A VRD R A   RDR GY+F PPGW+P
Sbjct  248  VPVTTHNPFRLQYGEFLAAVRDVRGARSWRDRAGYLFAPPGWKP  291


>gi|291191880|gb|ADD82987.1| unknown [Streptomyces platensis]
Length=300

 Score =  275 bits (703),  Expect = 6e-72, Method: Compositional matrix adjust.
 Identities = 147/296 (50%), Positives = 201/296 (68%), Gaps = 10/296 (3%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            +RDP++FA+P   LL+ +E  A R          G  + A G +  RD+ AS+  GL ++
Sbjct  9    LRDPIVFALPAVGLLVAIEMLALR---------FGDDKDAKG-FDARDTRASVLTGLGAL  58

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
              +   ++ AL  YA +Y YLAP+ L A  W+TWV+   GV+ L Y YHR AH +R+IWA
Sbjct  59   VVSTLLRAAALGLYAVVYTYLAPFHLPADAWWTWVVLFFGVEFLLYWYHRSAHMIRIIWA  118

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
             HQ HHSSEYFN +TALR+KW    E L+W+PLPL+G+PP +VF   S +LIY  + HTE
Sbjct  119  GHQVHHSSEYFNTSTALRRKWAQWFEKLVWLPLPLLGVPPVLVFTMHSFHLIYGLFAHTE  178

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            ++ +LPR  EF+F TPSHHRVHHG +P YLDKN+G ILIIWDR+FG+FQPE  RP YGLT
Sbjct  179  KVGKLPRPLEFLFVTPSHHRVHHGTEPEYLDKNFGSILIIWDRMFGTFQPEGRRPTYGLT  238

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTIDKSNAAASL  296
            K+++T++IWK+Q  E+  + R+ R A   R RLGY+FG PGW P +  + + + +L
Sbjct  239  KQINTYSIWKIQVHEFATMAREVRGADTWRHRLGYLFGRPGWRPESEKQQDTSPAL  294


>gi|315505770|ref|YP_004084657.1| fatty acid hydroxylase [Micromonospora sp. L5]
 gi|315412389|gb|ADU10506.1| fatty acid hydroxylase [Micromonospora sp. L5]
Length=275

 Score =  273 bits (697),  Expect = 3e-71, Method: Compositional matrix adjust.
 Identities = 142/284 (50%), Positives = 186/284 (66%), Gaps = 11/284 (3%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            M   VL+A+P FLLL+++E  + R L          P      Y  RD+  S+SMGL S 
Sbjct  1    MIPAVLYAVPAFLLLIVIEAVSYRFL----------PDDDERGYELRDTTTSLSMGLGSQ  50

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
                 WK L +  +AA++  +AP  LS   W+TWVI     DL YY +HR  H VR++WA
Sbjct  51   IIGFPWKLLTVGLFAALWT-VAPVHLSPGDWWTWVILFFADDLAYYWFHRSHHEVRVLWA  109

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            +H  HHSS Y+NF+TALRQ W     +  W+PL L+G+PPWM+F   S++L+YQF++HTE
Sbjct  110  SHVVHHSSVYYNFSTALRQSWTPMTSLPFWLPLALLGIPPWMIFLQQSVSLLYQFFLHTE  169

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            RI+ LPR  E++FNTPSHHRVHHG +  YLD+NYGGILI+WDRLFGSF+PE     YGLT
Sbjct  170  RINVLPRPIEWIFNTPSHHRVHHGSNTEYLDRNYGGILIVWDRLFGSFEPERATARYGLT  229

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEP  284
            K + T+N  ++ T E+ AI  D R A   R RLGY+FG PGW+P
Sbjct  230  KNIGTYNPLRVATHEFAAIWSDVRRADSWRHRLGYLFGRPGWQP  273


>gi|302867826|ref|YP_003836463.1| fatty acid hydroxylase [Micromonospora aurantiaca ATCC 27029]
 gi|302570685|gb|ADL46887.1| fatty acid hydroxylase [Micromonospora aurantiaca ATCC 27029]
Length=275

 Score =  272 bits (695),  Expect = 5e-71, Method: Compositional matrix adjust.
 Identities = 142/284 (50%), Positives = 186/284 (66%), Gaps = 11/284 (3%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            M   VL+A+P FLLL+++E  + R L          P      Y  RD+  S+SMGL S 
Sbjct  1    MIPAVLYAVPAFLLLIVIEAVSYRFL----------PDDDERGYELRDTTTSLSMGLGSQ  50

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
                 WK L +  +AA++  +AP  LS   W+TWVI     DL YY +HR  H VR++WA
Sbjct  51   IIGFPWKLLTVGLFAALWT-VAPVHLSPGDWWTWVILFFADDLAYYWFHRSHHEVRVLWA  109

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            +H  HHSS Y+NF+TALRQ W     +  W+PL L+G+PPWM+F   S++L+YQF++HTE
Sbjct  110  SHVVHHSSVYYNFSTALRQSWTPMTSLPFWLPLALLGIPPWMIFLQQSVSLLYQFFLHTE  169

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            RI+ LPR  E++FNTPSHHRVHHG +  YLD+NYGGILI+WDRLFGSF+PE     YGLT
Sbjct  170  RINVLPRPIEWIFNTPSHHRVHHGSNIEYLDRNYGGILIVWDRLFGSFEPERATARYGLT  229

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEP  284
            K + T+N  ++ T E+ AI  D R A   R RLGY+FG PGW+P
Sbjct  230  KNIGTYNPLRVATHEFAAIWSDVRRADSWRHRLGYLFGRPGWQP  273


>gi|225791059|gb|ACO31263.1| unknown [Streptomyces platensis]
Length=300

 Score =  270 bits (689),  Expect = 3e-70, Method: Compositional matrix adjust.
 Identities = 144/296 (49%), Positives = 198/296 (67%), Gaps = 10/296 (3%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            +RDP++FA+P   LL+ +E  A R          G  +   G +  RD+ AS+  GL ++
Sbjct  9    LRDPIVFALPAVGLLVAVEMLALR---------FGDDKDVKG-FNARDTRASVLTGLGAL  58

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
              +   ++ AL  YA +Y YLAP+ L A  W+TWV+   GV+ L Y YHR AH +R+IWA
Sbjct  59   VVSTLLRAAALGLYAVVYTYLAPFHLPADAWWTWVVLFFGVEFLLYWYHRSAHMIRIIWA  118

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
             HQ HHSSEYFN +TALR+KW    E L+W+PLPL+G+PP +VF   S +LIY  + HTE
Sbjct  119  GHQVHHSSEYFNTSTALRRKWAQWFEKLVWLPLPLLGVPPVLVFTMHSFHLIYGLFAHTE  178

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            ++ +LPR  EF+F T SHHRVHHG +P YLDKN+G ILIIWDR+FG+FQPE  RP YGLT
Sbjct  179  KVGKLPRPLEFIFVTASHHRVHHGTEPEYLDKNFGSILIIWDRMFGTFQPEGRRPTYGLT  238

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTIDKSNAAASL  296
            K+++T++IWK+   E+  + R+ R A   R R+GY+FG PGW P +  + + + SL
Sbjct  239  KQINTYSIWKIHVHEFATMAREVRGADTWRHRMGYLFGRPGWRPESEKQQDTSPSL  294


>gi|326329354|ref|ZP_08195679.1| C-5 sterol desaturase (Sterol-C5-desaturase) [Nocardioidaceae 
bacterium Broad-1]
 gi|325952929|gb|EGD44944.1| C-5 sterol desaturase (Sterol-C5-desaturase) [Nocardioidaceae 
bacterium Broad-1]
Length=296

 Score =  264 bits (674),  Expect = 2e-68, Method: Compositional matrix adjust.
 Identities = 144/285 (51%), Positives = 188/285 (66%), Gaps = 10/285 (3%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            +++P+ +AIP F L +++E  A + L+  +  A          Y+ +D+  S+ MGL SI
Sbjct  8    LKNPLTYAIPFFALSILIELAALKWLDHDDNLA---------GYVFKDARTSVLMGLGSI  58

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
             +   +K L    YAAI+AY + WQ+    W+   I++V VDL YY  HR  HR R+ WA
Sbjct  59   VSMTVFKVLTFAVYAAIFAYASLWQIP-ETWWGLAISVVVVDLAYYWNHRFVHRTRVGWA  117

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
             HQAHHSSEY  FATALRQKWN   E   ++PLP +G+ P  ++ ++S+NLIYQF+VHTE
Sbjct  118  AHQAHHSSEYMTFATALRQKWNPWFEFFFFLPLPFLGISPAAIYIAFSINLIYQFFVHTE  177

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
             I RL R  E V NTPSHHRVHHG DP YLDKNY GILIIWDRLFG+FQPE+ RP YGLT
Sbjct  178  TIGRLWRPLELVLNTPSHHRVHHGSDPEYLDKNYAGILIIWDRLFGTFQPEVQRPTYGLT  237

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPR  285
             +VDT+N+ +L+   +  +  D RSA   R +L YVF PPGW P 
Sbjct  238  YKVDTYNLLRLEYGAFRELWNDVRSADGWRLKLSYVFRPPGWTPE  282


>gi|302524753|ref|ZP_07277095.1| predicted protein [Streptomyces sp. AA4]
 gi|302433648|gb|EFL05464.1| predicted protein [Streptomyces sp. AA4]
Length=284

 Score =  261 bits (666),  Expect = 1e-67, Method: Compositional matrix adjust.
 Identities = 128/284 (46%), Positives = 182/284 (65%), Gaps = 10/284 (3%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
             +DP+   +P   L+L+     A  L++++     + +     YL +D+  S++ GL+ +
Sbjct  3    FKDPIKRGLPAISLILV-----AEALDALQDQKGDERK----RYL-KDTATSVAQGLIGL  52

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
              ++  ++L  + Y  +Y ++AP +        W+I  + VD L Y YHR AH  RL+WA
Sbjct  53   GFSSALRALMTIPYTYLYEHVAPSKRKLQSPRDWLILFIAVDFLVYVYHRNAHERRLLWA  112

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
             HQ HHSS+Y N +TA R+KW+   E L+W PLPL+G PP  VF   + +L+Y  + H +
Sbjct  113  GHQVHHSSDYVNVSTAFRRKWSQWFEKLVWAPLPLLGYPPQAVFYMHAYHLVYGVFAHMD  172

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            +I+R+PRWFE VF +PSHHRVHHG++P YLDKNYG ILIIWD+LFG+FQ E  RP YGLT
Sbjct  173  KIERMPRWFEKVFVSPSHHRVHHGVEPKYLDKNYGSILIIWDKLFGTFQEEEERPTYGLT  232

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEP  284
            K V T N+  LQT E+  + RD R A   R+R+ Y+FGPPGWEP
Sbjct  233  KPVPTENVVGLQTFEFKDLWRDVRKARNARERVKYLFGPPGWEP  276


>gi|338532042|ref|YP_004665376.1| hypothetical protein LILAB_11945 [Myxococcus fulvus HW-1]
 gi|337258138|gb|AEI64298.1| hypothetical protein LILAB_11945 [Myxococcus fulvus HW-1]
Length=301

 Score =  254 bits (650),  Expect = 1e-65, Method: Compositional matrix adjust.
 Identities = 129/283 (46%), Positives = 175/283 (62%), Gaps = 10/283 (3%)

Query  3    DPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSIAT  62
            D +  AIP F L ++ E    +KL         + RP +G  L +D+ AS+SMGL ++A 
Sbjct  8    DLITLAIPVFALTVVAEALWVKKLRD-------EGRPVAGHTL-KDTAASLSMGLGNVAI  59

Query  63   TAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWATH  122
               WK +A  GY A+Y  L P ++       WV+  +  DL YY++HR+ H  R  WA+H
Sbjct  60   NVLWKGVAFAGYLALYQ-LTPLRM-GFGVLAWVLLFLADDLCYYAFHRVHHESRFFWASH  117

Query  123  QAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTERI  182
              HHSS+++N +TALRQ W      + W PL L+G  P M+    S++L+YQ+W+HTE I
Sbjct  118  VVHHSSQHYNLSTALRQTWTPPTSFVFWAPLALLGFHPVMIVAQQSISLLYQYWIHTEAI  177

Query  183  DRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLTKR  242
             RLPR  E+V NTPSHHRVHH  +P YLDKNY GILIIWDRLFG+F+PE  +P YGLTK 
Sbjct  178  GRLPRPLEWVLNTPSHHRVHHASNPRYLDKNYAGILIIWDRLFGTFEPEGEKPVYGLTKN  237

Query  243  VDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPR  285
            ++T+N  ++   E+ AI RD      L+ RL YVF  P W+P 
Sbjct  238  LETYNPVRIAFHEFAAIARDAAKPGSLKQRLSYVFRNPAWKPE  280


>gi|108759252|ref|YP_635021.1| hypothetical protein MXAN_6907 [Myxococcus xanthus DK 1622]
 gi|108463132|gb|ABF88317.1| conserved hypothetical protein [Myxococcus xanthus DK 1622]
Length=301

 Score =  253 bits (647),  Expect = 2e-65, Method: Compositional matrix adjust.
 Identities = 131/284 (47%), Positives = 176/284 (62%), Gaps = 14/284 (4%)

Query  3    DPVLFAIPCFLLLLILE--WTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            D +  AIP F L +I E  W   ++ E          RP  G +  +D++ASISMGL ++
Sbjct  8    DLITPAIPVFTLTVIAEALWVKKQRDEG---------RPMVG-HTWKDTIASISMGLGNV  57

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
            A    WK +A  GY A+Y +L P ++       WV+     DL YY++HR+ H  R+ WA
Sbjct  58   AINVLWKGVAFAGYLALY-HLTPLRMGTGV-LAWVLIFFADDLCYYAFHRVHHESRIFWA  115

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            +H  HHSS+++N +TALRQ W      + W PL L+G  P M+    S++L+YQ+W+HTE
Sbjct  116  SHVVHHSSQHYNLSTALRQTWTPPTSFVFWAPLALLGFHPVMIVVQQSVSLLYQYWIHTE  175

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
             I RLPR  E+V NTPSHHRVHH  +P YLDKNY GILIIWDRLFG+F+PE  +P YGLT
Sbjct  176  AIGRLPRPLEWVLNTPSHHRVHHASNPRYLDKNYAGILIIWDRLFGTFEPEGEKPVYGLT  235

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEP  284
            K ++TFN  ++   E+ AI RD      L+ RL YVF  P W+P
Sbjct  236  KNLETFNPVRIAFHEFAAITRDAAKPGSLKQRLSYVFRNPAWKP  279


>gi|291302624|ref|YP_003513902.1| fatty acid hydroxylase [Stackebrandtia nassauensis DSM 44728]
 gi|290571844|gb|ADD44809.1| fatty acid hydroxylase [Stackebrandtia nassauensis DSM 44728]
Length=291

 Score =  253 bits (646),  Expect = 2e-65, Method: Compositional matrix adjust.
 Identities = 140/293 (48%), Positives = 182/293 (63%), Gaps = 21/293 (7%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            M D V+FA+P F+L +++E        S   A     R     Y  RD+  S++MG  S 
Sbjct  1    MVDAVIFAVPFFVLFVVIE------ALSFHFAPDDDER----GYELRDAATSMTMGAGSQ  50

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQL-----SAHRWYTWVIA--IVGV----DLLYYSYH  109
                 WK  ++  YAA Y +LAP+ L     +A     W+IA  I+G+    DL YY +H
Sbjct  51   VVGFVWKFASIFVYAAAYKFLAPFDLDVVENAAIASSPWLIAAVIIGLFFADDLAYYWFH  110

Query  110  RIAHRVRLIWATHQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSL  169
            R  HR+R++WA+H  HHSS+Y+NF TALRQ W     +  W+PL L+G PPW++F   S+
Sbjct  111  RGHHRIRVLWASHVVHHSSQYYNFTTALRQSWTPMTTLPFWLPLALIGFPPWLIFLQQSI  170

Query  170  NLIYQFWVHTERIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQ  229
            +L YQF++HTER+D+L R  E V NTPSHHRVHHG D  YLDKNYGGILIIWDR F +F+
Sbjct  171  SLGYQFFLHTERVDKLWRPVEAVMNTPSHHRVHHGSDDQYLDKNYGGILIIWDRAFRTFE  230

Query  230  PELFRPHYGLTKRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGW  282
            PE  R  YGLT+ ++TFN  ++   EY AI RD   A   R R GYVFGPPGW
Sbjct  231  PEGARVTYGLTRNIETFNPLRVAFHEYAAIWRDVIHARSWRARAGYVFGPPGW  283


>gi|256389551|ref|YP_003111115.1| fatty acid hydroxylase [Catenulispora acidiphila DSM 44928]
 gi|256355777|gb|ACU69274.1| fatty acid hydroxylase [Catenulispora acidiphila DSM 44928]
Length=289

 Score =  251 bits (642),  Expect = 7e-65, Method: Compositional matrix adjust.
 Identities = 127/284 (45%), Positives = 169/284 (60%), Gaps = 12/284 (4%)

Query  3    DPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSIAT  62
            D    AIP ++L + LE  + R            P P    Y  +D+  S++MG+ SI  
Sbjct  9    DLTTLAIPFYVLFMALELLSLRFF----------PDPVEQGYTVKDTATSLTMGIGSIVC  58

Query  63   TAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWATH  122
              GW  + +   A ++A LAPW + A     W++  V  D  YY  HR  HRVR++WA+H
Sbjct  59   NLGWGLVTVAAVAGVHA-LAPWHI-ASSLTGWLVLFVAYDFFYYWEHRANHRVRILWASH  116

Query  123  QAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTERI  182
              HHSS ++N +TALRQ W   G  +  +PL L+G P +M+F + ++NL+YQFW+HTERI
Sbjct  117  VVHHSSRHYNLSTALRQTWTGVGNTIFLLPLALVGFPSYMIFATAAMNLLYQFWIHTERI  176

Query  183  DRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLTKR  242
              + R  EFV NTPSHHRVHHG +  YLDKNYGG+ I++DRLF SF+PE     YGLTK 
Sbjct  177  ATMWRPIEFVMNTPSHHRVHHGSNQGYLDKNYGGVFIVFDRLFRSFEPEGEPVEYGLTKN  236

Query  243  VDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRT  286
            + TFN  ++   EY AI RD R A   R R GYV   PGW P +
Sbjct  237  IGTFNPLRVAAHEYAAIARDVRGAAGWRARFGYVLRGPGWSPAS  280


>gi|29832988|ref|NP_827622.1| sterol desaturase-related protein [Streptomyces avermitilis MA-4680]
 gi|29610109|dbj|BAC74157.1| putative sterol desaturase-related protein [Streptomyces avermitilis 
MA-4680]
Length=289

 Score =  248 bits (634),  Expect = 7e-64, Method: Compositional matrix adjust.
 Identities = 135/285 (48%), Positives = 178/285 (63%), Gaps = 14/285 (4%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            + D VL++IP F+LL ++E  + R            P   +  Y T+D+  S+ MGL S+
Sbjct  4    LPDVVLWSIPAFVLLTVIEIVSVRI----------HPEEDAEGYETKDAATSVGMGLGSL  53

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
                 WK   +  Y AIY  L P ++    W+T  + ++  D  YY  HR  H +R++WA
Sbjct  54   VFDLLWKIPIVAVYTAIYE-LTPLRVPV-LWWTVPLMLLAQDFFYYWSHRGHHVIRILWA  111

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGL-PPWMVFCSWSLNLIYQFWVHT  179
             H  HHSS  FN  TALRQ W +      +VPL  +G+ P  + FCS S+NL+YQFW+HT
Sbjct  112  CHVVHHSSRKFNLTTALRQPWTSLTVWPFYVPLIALGVHPAALAFCS-SVNLVYQFWIHT  170

Query  180  ERIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGL  239
            ERID+LPR FEFVFNTPSHHRVHH     YLD+N+GGILI+WDR+FGSF PE  RP YGL
Sbjct  171  ERIDKLPRAFEFVFNTPSHHRVHHASQGGYLDRNFGGILIVWDRIFGSFVPETERPVYGL  230

Query  240  TKRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEP  284
            TK + T+N  ++ T EYVAI +D R+A   R+R G VF  PGW+P
Sbjct  231  TKNIATYNPLRVATHEYVAIAKDLRAARGWRERAGRVFRGPGWQP  275


>gi|302550498|ref|ZP_07302840.1| fatty acid hydroxylase superfamily protein [Streptomyces viridochromogenes 
DSM 40736]
 gi|302468116|gb|EFL31209.1| fatty acid hydroxylase superfamily protein [Streptomyces viridochromogenes 
DSM 40736]
Length=314

 Score =  248 bits (633),  Expect = 8e-64, Method: Compositional matrix adjust.
 Identities = 139/303 (46%), Positives = 183/303 (61%), Gaps = 17/303 (5%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            + D VL++IP F+LL ++E  + R            P   +  Y  +D+  S+ MGL S+
Sbjct  4    LPDVVLWSIPAFVLLTVVEMVSCRL----------HPDEDAEGYEAKDAATSVGMGLGSL  53

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
                 WK   +  Y A+Y  L P ++    W+T  + ++  D  YY  HR  H +R++WA
Sbjct  54   VFDFLWKIPIVAVYTAVYE-LTPLRVPV-LWWTVPLMLLAQDFFYYWSHRGHHVIRILWA  111

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGL-PPWMVFCSWSLNLIYQFWVHT  179
             H  HHSS  FNF TALRQ W +      ++PL   G+ P  + FCS S NL+YQFW+HT
Sbjct  112  CHVVHHSSRKFNFTTALRQPWTSLTVWPFYLPLIAAGVHPAALAFCS-SANLVYQFWIHT  170

Query  180  ERIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGL  239
            ERID++PRWFEFV NTPSHHRVHH     YLD+N+GGILI+WDRLFGSF  E  RP YGL
Sbjct  171  ERIDKMPRWFEFVLNTPSHHRVHHASQGGYLDRNFGGILIVWDRLFGSFVAETTRPVYGL  230

Query  240  TKRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEP---RTIDKSNAAASL  296
            TK +DT+N  K+ T EYV+I +D R+A   R+R G VF  PGW P   R    S AA+ +
Sbjct  231  TKNIDTYNPLKVATHEYVSIAKDLRAARDWRERAGRVFRGPGWGPAPARAEVASPAASPV  290

Query  297  VTS  299
            V S
Sbjct  291  VES  293


>gi|297198749|ref|ZP_06916146.1| C-5 sterol desaturase [Streptomyces sviceus ATCC 29083]
 gi|197715368|gb|EDY59402.1| C-5 sterol desaturase [Streptomyces sviceus ATCC 29083]
Length=288

 Score =  246 bits (628),  Expect = 3e-63, Method: Compositional matrix adjust.
 Identities = 134/291 (47%), Positives = 178/291 (62%), Gaps = 14/291 (4%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            + D V+++IP F+LL ++E  + R            P   +  Y  +D+  SI MGL S+
Sbjct  4    LPDVVVWSIPAFVLLTVIEIISVRI----------HPDEDAAGYEAKDTATSIGMGLGSL  53

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
                 WK   +  Y A+Y +  P ++    W+T  + ++G D  YY  HR  H +R++WA
Sbjct  54   FFDFFWKIPIVAVYTALYEF-TPLRVPV-LWWTVPLMLLGQDFFYYWSHRGHHVIRILWA  111

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGL-PPWMVFCSWSLNLIYQFWVHT  179
             H  HHSS  FN  TALRQ W        +VPL   G+ P  + FCS S NL+YQFW+HT
Sbjct  112  CHVVHHSSRKFNLTTALRQPWTTLTVWPFYVPLVAAGVHPAALAFCS-SANLVYQFWIHT  170

Query  180  ERIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGL  239
            ERID++PRWFEFVFNTPSHHRVHH     YLD+N+GGILI+WDRLFGSF  E  RP YGL
Sbjct  171  ERIDKMPRWFEFVFNTPSHHRVHHASQGGYLDRNFGGILIVWDRLFGSFVAETERPVYGL  230

Query  240  TKRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEPRTIDKS  290
            TK ++TFN  K+ T EYVAI++D + A   R+R G VF  PGW P  + ++
Sbjct  231  TKNINTFNPIKVATHEYVAILKDIKRARSWRERAGRVFRGPGWAPAPLPET  281


>gi|328881514|emb|CCA54753.1| sterol desaturase-related protein [Streptomyces venezuelae ATCC 
10712]
Length=284

 Score =  245 bits (626),  Expect = 6e-63, Method: Compositional matrix adjust.
 Identities = 131/282 (47%), Positives = 176/282 (63%), Gaps = 12/282 (4%)

Query  3    DPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSIAT  62
            D VL++IP F+LL +LE  + R            P   +  Y T+D+  SI MGL S+A 
Sbjct  6    DVVLWSIPAFVLLTVLEMISHRI----------HPDEDAAGYETKDAATSIGMGLGSLAF  55

Query  63   TAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWATH  122
             A WK   +  Y A+Y  L P ++    W+T  + ++  D LYY  HR  H +R++WA H
Sbjct  56   DALWKIPVVALYTAVYE-LTPLRVPV-LWWTIPLMLLAQDFLYYWQHRGHHVIRVLWACH  113

Query  123  QAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTERI  182
              HHSS  FN +TALRQ W        ++P+  +G+ P  V   +S+NL+YQFW+HTERI
Sbjct  114  VVHHSSRKFNLSTALRQPWTGVTSWPFYLPMIALGVHPAAVAFCYSVNLVYQFWIHTERI  173

Query  183  DRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLTKR  242
            D+LPR FE+V NTPSHHRVHH     YLD+N+GGILI+WDR+FGS+  E  RP YGLTK 
Sbjct  174  DKLPRPFEYVLNTPSHHRVHHASQGGYLDRNFGGILIVWDRMFGSWTGETERPVYGLTKN  233

Query  243  VDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEP  284
            ++T+N  ++ T EY AI RD R+A   R+R G VF  PGW+P
Sbjct  234  IETYNPLRVATHEYAAIARDLRAAHDWRERAGRVFRGPGWQP  275


>gi|54023597|ref|YP_117839.1| hypothetical protein nfa16290 [Nocardia farcinica IFM 10152]
 gi|54015105|dbj|BAD56475.1| hypothetical protein [Nocardia farcinica IFM 10152]
Length=274

 Score =  244 bits (623),  Expect = 1e-62, Method: Compositional matrix adjust.
 Identities = 134/284 (48%), Positives = 174/284 (62%), Gaps = 12/284 (4%)

Query  1    MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSI  60
            M D +  AIP F++ L+LE  +   L          P      Y  RD+V S+ MGL ++
Sbjct  1    MFDLIAHAIPVFVVCLVLEAVSFHLL----------PDDDELGYHARDTVTSLLMGLGNV  50

Query  61   ATTAGWKSLALLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWA  120
                GWK + +    A     +P  L      TWV+     D +YY YHR  H VRL WA
Sbjct  51   VINIGWKLVVVA-VYAAVYLASPLHLPTDHPLTWVLLFFADDFVYYWYHRTHHTVRLFWA  109

Query  121  THQAHHSSEYFNFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTE  180
            +H AHHSS +FN +TALRQ W     +  W+PL L+  PPWMV    S++L+YQF++HTE
Sbjct  110  SHVAHHSSRFFNLSTALRQTWTPFTALPYWLPLALV-FPPWMVLLQQSISLLYQFFIHTE  168

Query  181  RIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPELFRPHYGLT  240
            R+  L R  E+VF+TPSHHRVHHG + VYLD+NYGGILI+WDRLFG+F+PE  R  YGLT
Sbjct  169  RVGTLWRPIEWVFDTPSHHRVHHGANDVYLDRNYGGILIVWDRLFGTFEPEGERVRYGLT  228

Query  241  KRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPGWEP  284
            K +DTFN  ++ T E+VAI RD R+A   R R G++F  PGW P
Sbjct  229  KNIDTFNPVRVATHEFVAIWRDVRAARDWRTRFGHLFRGPGWAP  272



Lambda     K      H
   0.326    0.139    0.473 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Effective search space used: 505145727648


  Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
    Posted date:  Sep 5, 2011  4:36 AM
  Number of letters in database: 5,219,829,388
  Number of sequences in database:  15,229,318



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40