BLASTP 2.2.25+
Reference:
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database
search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
15,229,318 sequences; 5,219,829,388 total letters
Query= Rv1893
Length=72
Score E
Sequences producing significant alignments: (Bits) Value
gi|15609030|ref|NP_216409.1| hypothetical protein Rv1893 [Mycoba... 139 1e-31
gi|240170540|ref|ZP_04749199.1| hypothetical protein MkanA1_1460... 115 2e-24
gi|41407715|ref|NP_960551.1| hypothetical protein MAP1617 [Mycob... 110 5e-23
gi|254822530|ref|ZP_05227531.1| hypothetical protein MintA_21554... 110 5e-23
gi|342859542|ref|ZP_08716195.1| hypothetical protein MCOL_11703 ... 107 5e-22
gi|118616861|ref|YP_905193.1| hypothetical protein MUL_1142 [Myc... 106 9e-22
gi|296164914|ref|ZP_06847470.1| conserved hypothetical protein [... 103 8e-21
gi|15828094|ref|NP_302357.1| hypothetical protein ML2022 [Mycoba... 98.2 3e-19
gi|4582350|emb|CAB40293.1| hypothetical protein MLCB561.11 [Myco... 96.7 9e-19
gi|333991121|ref|YP_004523735.1| hypothetical protein JDM601_248... 94.0 7e-18
gi|333921982|ref|YP_004495563.1| hypothetical protein AS9A_4330 ... 50.1 1e-04
gi|296140165|ref|YP_003647408.1| hypothetical protein Tpau_2464 ... 42.7 0.018
gi|256423262|ref|YP_003123915.1| amidase [Chitinophaga pinensis ... 39.3 0.17
gi|298706849|emb|CBJ25813.1| Glycosyltransferase, family GT49 [E... 37.7 0.62
gi|299136689|ref|ZP_07029872.1| Amidase [Acidobacterium sp. MP5A... 36.2 1.6
gi|91790145|ref|YP_551097.1| heavy metal translocating P-type AT... 34.7 5.0
gi|28211049|ref|NP_781993.1| ferric enterobactin transport ATP-b... 34.7 5.0
>gi|15609030|ref|NP_216409.1| hypothetical protein Rv1893 [Mycobacterium tuberculosis H37Rv]
gi|15841363|ref|NP_336400.1| hypothetical protein MT1943 [Mycobacterium tuberculosis CDC1551]
gi|31793085|ref|NP_855578.1| hypothetical protein Mb1926 [Mycobacterium bovis AF2122/97]
78 more sequence titles
Length=72
Score = 139 bits (350), Expect = 1e-31, Method: Compositional matrix adjust.
Identities = 72/72 (100%), Positives = 72/72 (100%), Gaps = 0/72 (0%)
Query 1 MSFNPKDAVDAVRDIAANAVEKASDIVENAGHIIRGDIAGGASGIVKDSIDIATHAVDRT 60
MSFNPKDAVDAVRDIAANAVEKASDIVENAGHIIRGDIAGGASGIVKDSIDIATHAVDRT
Sbjct 1 MSFNPKDAVDAVRDIAANAVEKASDIVENAGHIIRGDIAGGASGIVKDSIDIATHAVDRT 60
Query 61 KEVFTGKTDDEG 72
KEVFTGKTDDEG
Sbjct 61 KEVFTGKTDDEG 72
>gi|240170540|ref|ZP_04749199.1| hypothetical protein MkanA1_14600 [Mycobacterium kansasii ATCC
12478]
Length=72
Score = 115 bits (288), Expect = 2e-24, Method: Compositional matrix adjust.
Identities = 60/70 (86%), Positives = 63/70 (90%), Gaps = 0/70 (0%)
Query 1 MSFNPKDAVDAVRDIAANAVEKASDIVENAGHIIRGDIAGGASGIVKDSIDIATHAVDRT 60
M+ NPKDAVDA +DIA NAVEKASDIVENAG IIRGDIAGGASGIV+ SIDIATHAVDR
Sbjct 1 MAINPKDAVDAAKDIATNAVEKASDIVENAGDIIRGDIAGGASGIVQSSIDIATHAVDRV 60
Query 61 KEVFTGKTDD 70
KEVFTGK DD
Sbjct 61 KEVFTGKDDD 70
>gi|41407715|ref|NP_960551.1| hypothetical protein MAP1617 [Mycobacterium avium subsp. paratuberculosis
K-10]
gi|118464696|ref|YP_881995.1| hypothetical protein MAV_2808 [Mycobacterium avium 104]
gi|254775288|ref|ZP_05216804.1| hypothetical protein MaviaA2_11541 [Mycobacterium avium subsp.
avium ATCC 25291]
gi|41396068|gb|AAS03934.1| hypothetical protein MAP_1617 [Mycobacterium avium subsp. paratuberculosis
K-10]
gi|118165983|gb|ABK66880.1| conserved hypothetical protein [Mycobacterium avium 104]
gi|336457554|gb|EGO36560.1| hypothetical protein MAPs_22260 [Mycobacterium avium subsp. paratuberculosis
S397]
Length=76
Score = 110 bits (276), Expect = 5e-23, Method: Compositional matrix adjust.
Identities = 57/69 (83%), Positives = 63/69 (92%), Gaps = 0/69 (0%)
Query 1 MSFNPKDAVDAVRDIAANAVEKASDIVENAGHIIRGDIAGGASGIVKDSIDIATHAVDRT 60
M+FNPKDAVDA RDIA NAVEKASDIVE+A IIRGDIAGGASGIV++SIDI T+AVDRT
Sbjct 1 MAFNPKDAVDAARDIATNAVEKASDIVEHASDIIRGDIAGGASGIVQNSIDIGTYAVDRT 60
Query 61 KEVFTGKTD 69
KEVFTG+ D
Sbjct 61 KEVFTGRHD 69
>gi|254822530|ref|ZP_05227531.1| hypothetical protein MintA_21554 [Mycobacterium intracellulare
ATCC 13950]
Length=73
Score = 110 bits (276), Expect = 5e-23, Method: Compositional matrix adjust.
Identities = 55/69 (80%), Positives = 63/69 (92%), Gaps = 0/69 (0%)
Query 1 MSFNPKDAVDAVRDIAANAVEKASDIVENAGHIIRGDIAGGASGIVKDSIDIATHAVDRT 60
M+FNPKDAVDA RDIA NAVEKASDIVE+A IIRGD+AGGASGIV++SIDI T+A+DRT
Sbjct 1 MAFNPKDAVDAARDIATNAVEKASDIVEHASDIIRGDLAGGASGIVQNSIDIGTYALDRT 60
Query 61 KEVFTGKTD 69
KEVFTG+ D
Sbjct 61 KEVFTGRQD 69
>gi|342859542|ref|ZP_08716195.1| hypothetical protein MCOL_11703 [Mycobacterium colombiense CECT
3035]
gi|342132674|gb|EGT85894.1| hypothetical protein MCOL_11703 [Mycobacterium colombiense CECT
3035]
Length=73
Score = 107 bits (268), Expect = 5e-22, Method: Compositional matrix adjust.
Identities = 54/67 (81%), Positives = 61/67 (92%), Gaps = 0/67 (0%)
Query 1 MSFNPKDAVDAVRDIAANAVEKASDIVENAGHIIRGDIAGGASGIVKDSIDIATHAVDRT 60
M+FNPKDAVDA RDIA NAVEKASDIVE+A IIRGD+AGGASGIV +SIDI T+A+DRT
Sbjct 1 MAFNPKDAVDAARDIATNAVEKASDIVEHASDIIRGDLAGGASGIVANSIDIGTYALDRT 60
Query 61 KEVFTGK 67
KEVFTG+
Sbjct 61 KEVFTGR 67
>gi|118616861|ref|YP_905193.1| hypothetical protein MUL_1142 [Mycobacterium ulcerans Agy99]
gi|183982792|ref|YP_001851083.1| hypothetical protein MMAR_2786 [Mycobacterium marinum M]
gi|118568971|gb|ABL03722.1| conserved secreted protein [Mycobacterium ulcerans Agy99]
gi|183176118|gb|ACC41228.1| conserved secreted protein [Mycobacterium marinum M]
Length=72
Score = 106 bits (265), Expect = 9e-22, Method: Compositional matrix adjust.
Identities = 54/70 (78%), Positives = 61/70 (88%), Gaps = 0/70 (0%)
Query 1 MSFNPKDAVDAVRDIAANAVEKASDIVENAGHIIRGDIAGGASGIVKDSIDIATHAVDRT 60
M+ NPKDAV+A RDIA NAVEKASDIVENA IIRGD++GGASGIV++SIDIATHAVD+
Sbjct 1 MAINPKDAVNAARDIATNAVEKASDIVENASDIIRGDVSGGASGIVQNSIDIATHAVDKV 60
Query 61 KEVFTGKTDD 70
KEV GK DD
Sbjct 61 KEVLIGKDDD 70
>gi|296164914|ref|ZP_06847470.1| conserved hypothetical protein [Mycobacterium parascrofulaceum
ATCC BAA-614]
gi|295899756|gb|EFG79206.1| conserved hypothetical protein [Mycobacterium parascrofulaceum
ATCC BAA-614]
Length=74
Score = 103 bits (257), Expect = 8e-21, Method: Compositional matrix adjust.
Identities = 54/73 (74%), Positives = 63/73 (87%), Gaps = 2/73 (2%)
Query 1 MSFNPKDAVDAVRDIAANAVEKASDIVENAGHIIRGDIAGGASGIVKDSIDIATHAVDRT 60
M F PKDAVDA RDIA NAVEKASDIVE+A IIRGDI+GGASGIV++SIDI T+AVDRT
Sbjct 1 MGFKPKDAVDAARDIATNAVEKASDIVEHASDIIRGDISGGASGIVQNSIDIGTYAVDRT 60
Query 61 KEVFTGK--TDDE 71
+E+F+G+ DDE
Sbjct 61 REMFSGRDELDDE 73
>gi|15828094|ref|NP_302357.1| hypothetical protein ML2022 [Mycobacterium leprae TN]
gi|221230571|ref|YP_002503987.1| hypothetical protein MLBr_02022 [Mycobacterium leprae Br4923]
gi|13093648|emb|CAC30977.1| conserved hypothetical protein [Mycobacterium leprae]
gi|219933678|emb|CAR72119.1| conserved hypothetical protein [Mycobacterium leprae Br4923]
Length=174
Score = 98.2 bits (243), Expect = 3e-19, Method: Compositional matrix adjust.
Identities = 48/67 (72%), Positives = 58/67 (87%), Gaps = 0/67 (0%)
Query 1 MSFNPKDAVDAVRDIAANAVEKASDIVENAGHIIRGDIAGGASGIVKDSIDIATHAVDRT 60
M+F+PK+AV+A RDIA N VEKASDIVEN II+GDIAGGA+ IV++SIDIATHAVD+
Sbjct 101 MTFDPKNAVNAARDIATNFVEKASDIVENVSDIIKGDIAGGANDIVQNSIDIATHAVDKA 160
Query 61 KEVFTGK 67
KE+F GK
Sbjct 161 KEIFIGK 167
>gi|4582350|emb|CAB40293.1| hypothetical protein MLCB561.11 [Mycobacterium leprae]
Length=74
Score = 96.7 bits (239), Expect = 9e-19, Method: Compositional matrix adjust.
Identities = 48/67 (72%), Positives = 58/67 (87%), Gaps = 0/67 (0%)
Query 1 MSFNPKDAVDAVRDIAANAVEKASDIVENAGHIIRGDIAGGASGIVKDSIDIATHAVDRT 60
M+F+PK+AV+A RDIA N VEKASDIVEN II+GDIAGGA+ IV++SIDIATHAVD+
Sbjct 1 MTFDPKNAVNAARDIATNFVEKASDIVENVSDIIKGDIAGGANDIVQNSIDIATHAVDKA 60
Query 61 KEVFTGK 67
KE+F GK
Sbjct 61 KEIFIGK 67
>gi|333991121|ref|YP_004523735.1| hypothetical protein JDM601_2481 [Mycobacterium sp. JDM601]
gi|333487089|gb|AEF36481.1| conserved hypothetical protein [Mycobacterium sp. JDM601]
Length=76
Score = 94.0 bits (232), Expect = 7e-18, Method: Compositional matrix adjust.
Identities = 46/67 (69%), Positives = 58/67 (87%), Gaps = 0/67 (0%)
Query 1 MSFNPKDAVDAVRDIAANAVEKASDIVENAGHIIRGDIAGGASGIVKDSIDIATHAVDRT 60
M+ PKDA +A RDIA +AVEKASDIVE+AG IIRGD++GG + IV+DS++IATHAV+RT
Sbjct 1 MAITPKDAFNAARDIATHAVEKASDIVEDAGDIIRGDVSGGVNAIVQDSMEIATHAVERT 60
Query 61 KEVFTGK 67
KEVFT +
Sbjct 61 KEVFTDR 67
>gi|333921982|ref|YP_004495563.1| hypothetical protein AS9A_4330 [Amycolicicoccus subflavus DQS3-9A1]
gi|333484203|gb|AEF42763.1| hypothetical protein AS9A_4330 [Amycolicicoccus subflavus DQS3-9A1]
Length=92
Score = 50.1 bits (118), Expect = 1e-04, Method: Compositional matrix adjust.
Identities = 23/61 (38%), Positives = 40/61 (66%), Gaps = 0/61 (0%)
Query 5 PKDAVDAVRDIAANAVEKASDIVENAGHIIRGDIAGGASGIVKDSIDIATHAVDRTKEVF 64
P + VDA++ IA +++K+ +IVE A ++G+++GG I+K S DIAT+A + +
Sbjct 11 PTNTVDAMKHIAQTSLDKSGEIVEGAADTLKGNVSGGIGKIIKSSTDIATNAAGKGTRIL 70
Query 65 T 65
T
Sbjct 71 T 71
>gi|296140165|ref|YP_003647408.1| hypothetical protein Tpau_2464 [Tsukamurella paurometabola DSM
20162]
gi|296028299|gb|ADG79069.1| hypothetical protein Tpau_2464 [Tsukamurella paurometabola DSM
20162]
Length=87
Score = 42.7 bits (99), Expect = 0.018, Method: Compositional matrix adjust.
Identities = 22/51 (44%), Positives = 32/51 (63%), Gaps = 0/51 (0%)
Query 6 KDAVDAVRDIAANAVEKASDIVENAGHIIRGDIAGGASGIVKDSIDIATHA 56
KD +DA ++A N+V ++ +IVE A +RGD+ GG I + DIAT A
Sbjct 7 KDNIDAAVNVATNSVARSGEIVEGAAQALRGDVKGGIGKIATSATDIATTA 57
>gi|256423262|ref|YP_003123915.1| amidase [Chitinophaga pinensis DSM 2588]
gi|256038170|gb|ACU61714.1| Amidase [Chitinophaga pinensis DSM 2588]
Length=475
Score = 39.3 bits (90), Expect = 0.17, Method: Compositional matrix adjust.
Identities = 29/72 (41%), Positives = 40/72 (56%), Gaps = 8/72 (11%)
Query 2 SFNPKDAVDAVRDIAANAVEKASDIVENAGHIIRGDIAGGASGI---VKDSIDIATHAVD 58
+FNP A++A+ IA NA+E A ENA ++RG+ G G+ VKDSID A
Sbjct 43 AFNP--AINAIVTIADNAMEVAKK-AENA--VLRGNELGPLHGVPFTVKDSIDTANILTQ 97
Query 59 RTKEVFTGKTDD 70
R +F G+ D
Sbjct 98 RGSPIFKGRIPD 109
>gi|298706849|emb|CBJ25813.1| Glycosyltransferase, family GT49 [Ectocarpus siliculosus]
Length=1541
Score = 37.7 bits (86), Expect = 0.62, Method: Composition-based stats.
Identities = 23/72 (32%), Positives = 36/72 (50%), Gaps = 6/72 (8%)
Query 7 DAVDAVRDIAANAVEKASDIVENAGHII------RGDIAGGASGIVKDSIDIATHAVDRT 60
+ +DA D+AA+ ++ A D+ E+ G + GD+AGGA + D++D VD
Sbjct 1118 EGLDAAGDLAADGLDAAGDLAEDIGEGMGDGLEAAGDLAGGAVDAITDAVDGVADDVDMY 1177
Query 61 KEVFTGKTDDEG 72
DDEG
Sbjct 1178 AGAGPRDLDDEG 1189
>gi|299136689|ref|ZP_07029872.1| Amidase [Acidobacterium sp. MP5ACTX8]
gi|298601204|gb|EFI57359.1| Amidase [Acidobacterium sp. MP5ACTX8]
Length=469
Score = 36.2 bits (82), Expect = 1.6, Method: Composition-based stats.
Identities = 27/72 (38%), Positives = 37/72 (52%), Gaps = 8/72 (11%)
Query 2 SFNPKDAVDAVRDIAANAVEKASDIVENAGHIIRGDIAGGASGI---VKDSIDIATHAVD 58
+ NPK V+A+ IA A+E A E ++RGD G G+ VKDSID A
Sbjct 37 AVNPK--VNAIVTIADGALESAK---EAEAAVLRGDELGPLHGVPFTVKDSIDTANVLTQ 91
Query 59 RTKEVFTGKTDD 70
R +F G+T +
Sbjct 92 RGSPIFKGRTPE 103
>gi|91790145|ref|YP_551097.1| heavy metal translocating P-type ATPase [Polaromonas sp. JS666]
gi|91699370|gb|ABE46199.1| Heavy metal translocating P-type ATPase [Polaromonas sp. JS666]
Length=760
Score = 34.7 bits (78), Expect = 5.0, Method: Composition-based stats.
Identities = 18/36 (50%), Positives = 23/36 (64%), Gaps = 0/36 (0%)
Query 11 AVRDIAANAVEKASDIVENAGHIIRGDIAGGASGIV 46
A+ A AV A +I E AGH ++G IAGGA+G V
Sbjct 508 AIVQAVAGAVPGAENITEIAGHGLQGQIAGGAAGRV 543
>gi|28211049|ref|NP_781993.1| ferric enterobactin transport ATP-binding protein fepC [Clostridium
tetani E88]
gi|28203488|gb|AAO35930.1| ferric enterobactin transport ATP-binding protein fepC [Clostridium
tetani E88]
Length=397
Score = 34.7 bits (78), Expect = 5.0, Method: Composition-based stats.
Identities = 20/72 (28%), Positives = 45/72 (63%), Gaps = 5/72 (6%)
Query 1 MSFNPKDAVDAVRDIAANAVEKASDIVENAGHIIRGDIAGGASGIVKDSIDIATHAVDRT 60
+S ++A ++++DI ++KA +++N +I D A I KD++ + ++A+++
Sbjct 304 LSVLKENAFESIKDIN---IKKAKSMIDNVSFVI--DSAYKIGEINKDNVQLISYALEQG 358
Query 61 KEVFTGKTDDEG 72
K+VF+ + +DEG
Sbjct 359 KKVFSFRKEDEG 370
Lambda K H
0.312 0.131 0.357
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 127392703088
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Sep 5, 2011 4:36 AM
Number of letters in database: 5,219,829,388
Number of sequences in database: 15,229,318
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40