BLASTP 2.2.25+
Reference:
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database
search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
15,229,318 sequences; 5,219,829,388 total letters
Query= Rv1914c
Length=135
Score E
Sequences producing significant alignments: (Bits) Value
gi|15609051|ref|NP_216430.1| hypothetical protein Rv1914c [Mycob... 273 4e-72
gi|323719577|gb|EGB28701.1| hypothetical protein TMMG_01174 [Myc... 248 1e-64
gi|254550930|ref|ZP_05141377.1| hypothetical protein Mtube_10796... 243 8e-63
gi|118618349|ref|YP_906681.1| hypothetical protein MUL_2941 [Myc... 178 2e-43
gi|183982824|ref|YP_001851115.1| hypothetical protein MMAR_2820 ... 174 3e-42
gi|240172358|ref|ZP_04751017.1| hypothetical protein MkanA1_2378... 122 2e-26
gi|317507100|ref|ZP_07964861.1| hypothetical protein HMPREF9336_... 82.0 2e-14
gi|148265072|ref|YP_001231778.1| hypothetical protein Gura_3033 ... 80.5 7e-14
gi|296395124|ref|YP_003660008.1| hypothetical protein Srot_2743 ... 79.3 2e-13
gi|343921194|gb|EGV31918.1| hypothetical protein ThidrDRAFT_1803... 64.3 6e-09
gi|170577352|ref|XP_001893973.1| Immunoglobulin I-set domain con... 35.8 2.0
gi|21752415|dbj|BAC04191.1| unnamed protein product [Homo sapiens] 35.0 3.2
gi|312067154|ref|XP_003136609.1| hypothetical protein LOAG_01021... 34.3 5.8
gi|242054577|ref|XP_002456434.1| hypothetical protein SORBIDRAFT... 34.3 6.7
gi|90023210|ref|YP_529037.1| hypothetical protein Sde_3570 [Sacc... 33.9 7.4
>gi|15609051|ref|NP_216430.1| hypothetical protein Rv1914c [Mycobacterium tuberculosis H37Rv]
gi|15841386|ref|NP_336423.1| hypothetical protein MT1965 [Mycobacterium tuberculosis CDC1551]
gi|31793107|ref|NP_855600.1| hypothetical protein Mb1949c [Mycobacterium bovis AF2122/97]
76 more sequence titles
Length=135
Score = 273 bits (699), Expect = 4e-72, Method: Compositional matrix adjust.
Identities = 135/135 (100%), Positives = 135/135 (100%), Gaps = 0/135 (0%)
Query 1 MVLSRTSTGRVILVPTQLRFDRWFLPLAVPLGLGPKNSELWVGAGSLHVKMGWAFAADIP 60
MVLSRTSTGRVILVPTQLRFDRWFLPLAVPLGLGPKNSELWVGAGSLHVKMGWAFAADIP
Sbjct 1 MVLSRTSTGRVILVPTQLRFDRWFLPLAVPLGLGPKNSELWVGAGSLHVKMGWAFAADIP 60
Query 61 LTSITKAEATNARVYAAGVHFGFGRWLVNGSRKGLVALTIDPPEQAKMWKKSMTVRELWV 120
LTSITKAEATNARVYAAGVHFGFGRWLVNGSRKGLVALTIDPPEQAKMWKKSMTVRELWV
Sbjct 61 LTSITKAEATNARVYAAGVHFGFGRWLVNGSRKGLVALTIDPPEQAKMWKKSMTVRELWV 120
Query 121 SVTDPDALVTACTAK 135
SVTDPDALVTACTAK
Sbjct 121 SVTDPDALVTACTAK 135
>gi|323719577|gb|EGB28701.1| hypothetical protein TMMG_01174 [Mycobacterium tuberculosis CDC1551A]
gi|339294849|gb|AEJ46960.1| hypothetical protein CCDC5079_1770 [Mycobacterium tuberculosis
CCDC5079]
gi|339298474|gb|AEJ50584.1| hypothetical protein CCDC5180_1747 [Mycobacterium tuberculosis
CCDC5180]
Length=122
Score = 248 bits (634), Expect = 1e-64, Method: Compositional matrix adjust.
Identities = 121/122 (99%), Positives = 122/122 (100%), Gaps = 0/122 (0%)
Query 14 VPTQLRFDRWFLPLAVPLGLGPKNSELWVGAGSLHVKMGWAFAADIPLTSITKAEATNAR 73
+PTQLRFDRWFLPLAVPLGLGPKNSELWVGAGSLHVKMGWAFAADIPLTSITKAEATNAR
Sbjct 1 MPTQLRFDRWFLPLAVPLGLGPKNSELWVGAGSLHVKMGWAFAADIPLTSITKAEATNAR 60
Query 74 VYAAGVHFGFGRWLVNGSRKGLVALTIDPPEQAKMWKKSMTVRELWVSVTDPDALVTACT 133
VYAAGVHFGFGRWLVNGSRKGLVALTIDPPEQAKMWKKSMTVRELWVSVTDPDALVTACT
Sbjct 61 VYAAGVHFGFGRWLVNGSRKGLVALTIDPPEQAKMWKKSMTVRELWVSVTDPDALVTACT 120
Query 134 AK 135
AK
Sbjct 121 AK 122
>gi|254550930|ref|ZP_05141377.1| hypothetical protein Mtube_10796 [Mycobacterium tuberculosis
'98-R604 INH-RIF-EM']
Length=135
Score = 243 bits (619), Expect = 8e-63, Method: Compositional matrix adjust.
Identities = 123/135 (92%), Positives = 123/135 (92%), Gaps = 0/135 (0%)
Query 1 MVLSRTSTGRVILVPTQLRFDRWFLPLAVPLGLGPKNSELWVGAGSLHVKMGWAFAADIP 60
MVLSRTSTGRVILVPTQLRFDRWFLPLAVPLGLGPKNSELWVGAGSLHVKMGWAFAADIP
Sbjct 1 MVLSRTSTGRVILVPTQLRFDRWFLPLAVPLGLGPKNSELWVGAGSLHVKMGWAFAADIP 60
Query 61 LTSITKAEATNARVYAAGVHFGFGRWLVNGSRKGLVALTIDPPEQAKMWKKSMTVRELWV 120
LTS AAGVHFGFGRWLVNGSRKGLVALTIDPPEQAKMWKKSMTVRELWV
Sbjct 61 LTSPPACGRAPRGFEAAGVHFGFGRWLVNGSRKGLVALTIDPPEQAKMWKKSMTVRELWV 120
Query 121 SVTDPDALVTACTAK 135
SVTDPDALVTACTAK
Sbjct 121 SVTDPDALVTACTAK 135
>gi|118618349|ref|YP_906681.1| hypothetical protein MUL_2941 [Mycobacterium ulcerans Agy99]
gi|118570459|gb|ABL05210.1| conserved hypothetical protein - truncated [Mycobacterium ulcerans
Agy99]
Length=122
Score = 178 bits (452), Expect = 2e-43, Method: Compositional matrix adjust.
Identities = 86/117 (74%), Positives = 98/117 (84%), Gaps = 0/117 (0%)
Query 16 TQLRFDRWFLPLAVPLGLGPKNSELWVGAGSLHVKMGWAFAADIPLTSITKAEATNARVY 75
TQLRF+RWFLPL+VPLGLGPKN + V AG+LHV+MGWAFAADIP+TSI A TNARV+
Sbjct 3 TQLRFERWFLPLSVPLGLGPKNCAVRVEAGNLHVRMGWAFAADIPVTSIKSAALTNARVF 62
Query 76 AAGVHFGFGRWLVNGSRKGLVALTIDPPEQAKMWKKSMTVRELWVSVTDPDALVTAC 132
AAGVH+ GRWLVNGS KGLVALTI+PP QAK S+ VR LW+SVTDPDAL+ AC
Sbjct 63 AAGVHYSGGRWLVNGSGKGLVALTIEPPAQAKAVFMSVRVRSLWISVTDPDALIAAC 119
>gi|183982824|ref|YP_001851115.1| hypothetical protein MMAR_2820 [Mycobacterium marinum M]
gi|183176150|gb|ACC41260.1| conserved hypothetical protein [Mycobacterium marinum M]
Length=122
Score = 174 bits (442), Expect = 3e-42, Method: Compositional matrix adjust.
Identities = 84/118 (72%), Positives = 96/118 (82%), Gaps = 0/118 (0%)
Query 16 TQLRFDRWFLPLAVPLGLGPKNSELWVGAGSLHVKMGWAFAADIPLTSITKAEATNARVY 75
TQLRF+RWFLPL+VPLGLGPKN + V AG+LHV+MGWAFAADIP+ SI A TNARV+
Sbjct 3 TQLRFERWFLPLSVPLGLGPKNCAVGVEAGNLHVRMGWAFAADIPVASIKSAALTNARVF 62
Query 76 AAGVHFGFGRWLVNGSRKGLVALTIDPPEQAKMWKKSMTVRELWVSVTDPDALVTACT 133
AAGVH+ GRWLVNGS KGLVAL I+PP QA S+ VR LW+SVTDPDAL+ ACT
Sbjct 63 AAGVHYSGGRWLVNGSGKGLVALMIEPPAQATAVFMSVRVRSLWISVTDPDALIAACT 120
>gi|240172358|ref|ZP_04751017.1| hypothetical protein MkanA1_23788 [Mycobacterium kansasii ATCC
12478]
Length=108
Score = 122 bits (305), Expect = 2e-26, Method: Compositional matrix adjust.
Identities = 60/107 (57%), Positives = 77/107 (72%), Gaps = 0/107 (0%)
Query 29 VPLGLGPKNSELWVGAGSLHVKMGWAFAADIPLTSITKAEATNARVYAAGVHFGFGRWLV 88
+PLG GPK+SE+ V G+L VK GW F A+IPL SI A+ N RVY+ G H GRWLV
Sbjct 1 MPLGCGPKHSEVRVQGGTLRVKFGWGFNAEIPLASIKDAKPNNERVYSWGAHGFRGRWLV 60
Query 89 NGSRKGLVALTIDPPEQAKMWKKSMTVRELWVSVTDPDALVTACTAK 135
NGS KG+V LT+DPP +AK+ +T++ L+VSVTDPDAL+ +AK
Sbjct 61 NGSSKGIVELTVDPPTRAKVMGVPVTLKTLYVSVTDPDALIAEVSAK 107
>gi|317507100|ref|ZP_07964861.1| hypothetical protein HMPREF9336_01232 [Segniliparus rugosus ATCC
BAA-974]
gi|316254594|gb|EFV13903.1| hypothetical protein HMPREF9336_01232 [Segniliparus rugosus ATCC
BAA-974]
Length=122
Score = 82.0 bits (201), Expect = 2e-14, Method: Compositional matrix adjust.
Identities = 45/116 (39%), Positives = 64/116 (56%), Gaps = 1/116 (0%)
Query 18 LRFDRWFLPLAVPLGLGPKNSELWVGAGSLHVKMGWAFAADIPLTSITKAEATNARVYAA 77
+R+DRW+ LA +GPK + + + LHV+ GW+F D+PL +I+ A R
Sbjct 5 MRYDRWYQLLATVFWMGPKRTVIRIVDDVLHVRHGWSFRIDVPLANISSARIYGKRPLGW 64
Query 78 GVHFGFGRWLVNGSRKGLVALTIDPP-EQAKMWKKSMTVRELWVSVTDPDALVTAC 132
GVH WLVNGSR G+V + D P + AK +R L +S+TDPD + A
Sbjct 65 GVHAFQNGWLVNGSRDGIVIVQFDAPIKPAKAPLFRWPIRSLAISLTDPDGFLAAL 120
>gi|148265072|ref|YP_001231778.1| hypothetical protein Gura_3033 [Geobacter uraniireducens Rf4]
gi|146398572|gb|ABQ27205.1| hypothetical protein Gura_3033 [Geobacter uraniireducens Rf4]
Length=135
Score = 80.5 bits (197), Expect = 7e-14, Method: Compositional matrix adjust.
Identities = 46/129 (36%), Positives = 73/129 (57%), Gaps = 2/129 (1%)
Query 5 RTSTGRVILVPTQ--LRFDRWFLPLAVPLGLGPKNSELWVGAGSLHVKMGWAFAADIPLT 62
R+ GR L P + ++FD W+ L+ L L P +S + V + + V+MGWAF A P
Sbjct 2 RSFIGRRPLSPQRFPIQFDPWYGILSSALFLRPSSSYVEVNSEEIRVRMGWAFRACFPRA 61
Query 63 SITKAEATNARVYAAGVHFGFGRWLVNGSRKGLVALTIDPPEQAKMWKKSMTVRELWVSV 122
++ A T+ R + GVH GRWLVNGS +G++ + + P ++ + + +R+L VSV
Sbjct 62 AVALAAETHGRPLSRGVHGFAGRWLVNGSGQGILTIDLTPTQRGYVMGFPVRLRQLMVSV 121
Query 123 TDPDALVTA 131
+P L A
Sbjct 122 AEPATLAAA 130
>gi|296395124|ref|YP_003660008.1| hypothetical protein Srot_2743 [Segniliparus rotundus DSM 44985]
gi|296182271|gb|ADG99177.1| hypothetical protein Srot_2743 [Segniliparus rotundus DSM 44985]
Length=167
Score = 79.3 bits (194), Expect = 2e-13, Method: Compositional matrix adjust.
Identities = 47/115 (41%), Positives = 62/115 (54%), Gaps = 1/115 (0%)
Query 18 LRFDRWFLPLAVPLGLGPKNSELWVGAGSLHVKMGWAFAADIPLTSITKAEATNARVYAA 77
+R+DRW PL+ G+GPK + + V L VK GWAF D+PL +I A R A
Sbjct 45 MRYDRWCRPLSTVFGMGPKRAVIRVDDNMLRVKHGWAFQIDVPLGNIASARLYGKRPLAW 104
Query 78 GVHFGFGRWLVNGSRKGLVALTIDPP-EQAKMWKKSMTVRELWVSVTDPDALVTA 131
GVH WLVNGSR G+V + P + K + VR + VS+ PDA + A
Sbjct 105 GVHGAEDGWLVNGSRDGVVIVRFATPVKPVKAPLGAWPVRCVLVSLEKPDAFLAA 159
>gi|343921194|gb|EGV31918.1| hypothetical protein ThidrDRAFT_1803 [Thiorhodococcus drewsii
AZ1]
Length=128
Score = 64.3 bits (155), Expect = 6e-09, Method: Compositional matrix adjust.
Identities = 40/109 (37%), Positives = 62/109 (57%), Gaps = 0/109 (0%)
Query 18 LRFDRWFLPLAVPLGLGPKNSELWVGAGSLHVKMGWAFAADIPLTSITKAEATNARVYAA 77
+RFD W+ L+ L L P S + + + V+MGWAF+A P T+I + N +
Sbjct 8 IRFDGWYEFLSTLLLLPPSTSYVSISRDLVEVRMGWAFSARFPRTAIASVASLNRPPVSR 67
Query 78 GVHFGFGRWLVNGSRKGLVALTIDPPEQAKMWKKSMTVRELWVSVTDPD 126
GVH GRWLVNGS +G++ L + P ++ + + +REL VS+ P+
Sbjct 68 GVHGFAGRWLVNGSGRGILTLDLKPAQRGYVMGFPVRLRELQVSLERPE 116
>gi|170577352|ref|XP_001893973.1| Immunoglobulin I-set domain containing protein [Brugia malayi]
gi|158599677|gb|EDP37190.1| Immunoglobulin I-set domain containing protein [Brugia malayi]
Length=4791
Score = 35.8 bits (81), Expect = 2.0, Method: Composition-based stats.
Identities = 15/36 (42%), Positives = 22/36 (62%), Gaps = 0/36 (0%)
Query 54 AFAADIPLTSITKAEATNARVYAAGVHFGFGRWLVN 89
AF +DIP T+IT+ E+ N +V G F +W +N
Sbjct 551 AFLSDIPATTITEGESLNVKVIITGDPTPFTKWYIN 586
>gi|21752415|dbj|BAC04191.1| unnamed protein product [Homo sapiens]
Length=158
Score = 35.0 bits (79), Expect = 3.2, Method: Compositional matrix adjust.
Identities = 18/49 (37%), Positives = 27/49 (56%), Gaps = 9/49 (18%)
Query 40 LWVGAGSLHVK----MGWAFAADIPLTSITKAEATNARVYAAGVHFGFG 84
+W GA +LHVK + W A+ IP+ AE+ N ++A +H G G
Sbjct 13 IWTGAENLHVKISCSLDWLMASVIPV-----AESRNLYIFADELHLGMG 56
>gi|312067154|ref|XP_003136609.1| hypothetical protein LOAG_01021 [Loa loa]
gi|307768228|gb|EFO27462.1| hypothetical protein LOAG_01021 [Loa loa]
Length=5884
Score = 34.3 bits (77), Expect = 5.8, Method: Composition-based stats.
Identities = 13/36 (37%), Positives = 22/36 (62%), Gaps = 0/36 (0%)
Query 54 AFAADIPLTSITKAEATNARVYAAGVHFGFGRWLVN 89
+F +DIP T++T+ E+ N +V G F +W +N
Sbjct 1467 SFLSDIPATTVTEGESLNVKVIVTGDPTPFTKWYIN 1502
>gi|242054577|ref|XP_002456434.1| hypothetical protein SORBIDRAFT_03g036240 [Sorghum bicolor]
gi|241928409|gb|EES01554.1| hypothetical protein SORBIDRAFT_03g036240 [Sorghum bicolor]
Length=1074
Score = 34.3 bits (77), Expect = 6.7, Method: Composition-based stats.
Identities = 13/33 (40%), Positives = 22/33 (67%), Gaps = 0/33 (0%)
Query 101 DPPEQAKMWKKSMTVRELWVSVTDPDALVTACT 133
DP ++A++WK+ M E + + +PDA+ ACT
Sbjct 297 DPDDEARLWKEHMNQIEATMVLLEPDAVARACT 329
>gi|90023210|ref|YP_529037.1| hypothetical protein Sde_3570 [Saccharophagus degradans 2-40]
gi|89952810|gb|ABD82825.1| type II secretion system protein N [Saccharophagus degradans
2-40]
Length=256
Score = 33.9 bits (76), Expect = 7.4, Method: Compositional matrix adjust.
Identities = 35/107 (33%), Positives = 47/107 (44%), Gaps = 26/107 (24%)
Query 43 GAGSLHVKMGWAFAADIPLTSITKAEA----TNARVYAAGVHFGFGRWLVN---GSRKGL 95
G GS+ V +A++ SI K +A NARV+A G F G + N R GL
Sbjct 141 GTGSIEV-----ISAEVTPQSIQKMDARVSWQNARVFADGTWFSLGSYAANVKENGRGGL 195
Query 96 VA--LTIDPPEQAKM---------WKKSMTVRELWVSVTDPDALVTA 131
A +D P Q K+ WK + TV+ L P+ LV A
Sbjct 196 AADVFDLDAPFQTKLNADWMANQGWKLNGTVKPL---SNAPELLVQA 239
Lambda K H
0.321 0.134 0.429
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 129391415580
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Sep 5, 2011 4:36 AM
Number of letters in database: 5,219,829,388
Number of sequences in database: 15,229,318
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40