BLASTP 2.2.25+
Reference:
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database
search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
15,229,318 sequences; 5,219,829,388 total letters
Query= Rv1946c
Length=150
Score E
Sequences producing significant alignments: (Bits) Value
gi|15609083|ref|NP_216462.1| lipoprotein [Mycobacterium tubercul... 297 3e-79
gi|31793138|ref|NP_855631.1| lipoprotein [Mycobacterium bovis AF... 296 7e-79
gi|289574629|ref|ZP_06454856.1| lipoprotein lppG [Mycobacterium ... 295 1e-78
gi|15609080|ref|NP_216459.1| hypothetical protein Rv1943c [Mycob... 43.9 0.009
gi|317501220|ref|ZP_07959425.1| cell division protein FtsW [Lach... 34.7 4.3
gi|153814620|ref|ZP_01967288.1| hypothetical protein RUMTOR_0083... 34.7 4.3
gi|238061142|ref|ZP_04605851.1| diguanylate cyclase/phosphodiest... 34.7 4.6
gi|315501454|ref|YP_004080341.1| GAF sensor-containing diguanyla... 34.3 6.6
gi|302869993|ref|YP_003838630.1| diguanylate cyclase [Micromonos... 34.3 6.7
>gi|15609083|ref|NP_216462.1| lipoprotein [Mycobacterium tuberculosis H37Rv]
gi|15841417|ref|NP_336454.1| hypothetical protein MT1997 [Mycobacterium tuberculosis CDC1551]
gi|148661754|ref|YP_001283277.1| putative lipoprotein LppG [Mycobacterium tuberculosis H37Ra]
40 more sequence titles
Length=150
Score = 297 bits (761), Expect = 3e-79, Method: Compositional matrix adjust.
Identities = 150/150 (100%), Positives = 150/150 (100%), Gaps = 0/150 (0%)
Query 1 MIRGSAVSGLLMPSVNGGTAGSVACVQCLFLPKVAVDLINLSGIQCFARIEHVAHAQAHP 60
MIRGSAVSGLLMPSVNGGTAGSVACVQCLFLPKVAVDLINLSGIQCFARIEHVAHAQAHP
Sbjct 1 MIRGSAVSGLLMPSVNGGTAGSVACVQCLFLPKVAVDLINLSGIQCFARIEHVAHAQAHP 60
Query 61 FVVLVGKPAQHGARIGAVAGAILTGDVIVSHDGELYRAVTALRQNGPRPHASRRLHAPAL 120
FVVLVGKPAQHGARIGAVAGAILTGDVIVSHDGELYRAVTALRQNGPRPHASRRLHAPAL
Sbjct 61 FVVLVGKPAQHGARIGAVAGAILTGDVIVSHDGELYRAVTALRQNGPRPHASRRLHAPAL 120
Query 121 CSARSRRGHLRPSCWLPPPRFAGRQSLVAR 150
CSARSRRGHLRPSCWLPPPRFAGRQSLVAR
Sbjct 121 CSARSRRGHLRPSCWLPPPRFAGRQSLVAR 150
>gi|31793138|ref|NP_855631.1| lipoprotein [Mycobacterium bovis AF2122/97]
gi|121637851|ref|YP_978074.1| putative lipoprotein [Mycobacterium bovis BCG str. Pasteur 1173P2]
gi|224990335|ref|YP_002645022.1| putative lipoprotein [Mycobacterium bovis BCG str. Tokyo 172]
gi|31618729|emb|CAD94683.1| POSSIBLE LIPOPROTEIN [Mycobacterium bovis AF2122/97]
gi|121493498|emb|CAL71972.1| Possible lipoprotein [Mycobacterium bovis BCG str. Pasteur 1173P2]
gi|224773448|dbj|BAH26254.1| putative lipoprotein [Mycobacterium bovis BCG str. Tokyo 172]
gi|341601878|emb|CCC64552.1| possible lipoprotein [Mycobacterium bovis BCG str. Moreau RDJ]
Length=150
Score = 296 bits (758), Expect = 7e-79, Method: Compositional matrix adjust.
Identities = 149/150 (99%), Positives = 149/150 (99%), Gaps = 0/150 (0%)
Query 1 MIRGSAVSGLLMPSVNGGTAGSVACVQCLFLPKVAVDLINLSGIQCFARIEHVAHAQAHP 60
MIRGSAVSGLLMPSVNGGTAGSVACVQCLFLPKVAVDLINLSGIQCFARIEHVAHAQAHP
Sbjct 1 MIRGSAVSGLLMPSVNGGTAGSVACVQCLFLPKVAVDLINLSGIQCFARIEHVAHAQAHP 60
Query 61 FVVLVGKPAQHGARIGAVAGAILTGDVIVSHDGELYRAVTALRQNGPRPHASRRLHAPAL 120
FVVLVGKPAQHGARIGAVAGAILTGDVIVSHDGELYR VTALRQNGPRPHASRRLHAPAL
Sbjct 61 FVVLVGKPAQHGARIGAVAGAILTGDVIVSHDGELYRTVTALRQNGPRPHASRRLHAPAL 120
Query 121 CSARSRRGHLRPSCWLPPPRFAGRQSLVAR 150
CSARSRRGHLRPSCWLPPPRFAGRQSLVAR
Sbjct 121 CSARSRRGHLRPSCWLPPPRFAGRQSLVAR 150
>gi|289574629|ref|ZP_06454856.1| lipoprotein lppG [Mycobacterium tuberculosis K85]
gi|289539060|gb|EFD43638.1| lipoprotein lppG [Mycobacterium tuberculosis K85]
Length=150
Score = 295 bits (756), Expect = 1e-78, Method: Compositional matrix adjust.
Identities = 149/150 (99%), Positives = 149/150 (99%), Gaps = 0/150 (0%)
Query 1 MIRGSAVSGLLMPSVNGGTAGSVACVQCLFLPKVAVDLINLSGIQCFARIEHVAHAQAHP 60
MIRGSAVSGLLMPSVNG TAGSVACVQCLFLPKVAVDLINLSGIQCFARIEHVAHAQAHP
Sbjct 1 MIRGSAVSGLLMPSVNGDTAGSVACVQCLFLPKVAVDLINLSGIQCFARIEHVAHAQAHP 60
Query 61 FVVLVGKPAQHGARIGAVAGAILTGDVIVSHDGELYRAVTALRQNGPRPHASRRLHAPAL 120
FVVLVGKPAQHGARIGAVAGAILTGDVIVSHDGELYRAVTALRQNGPRPHASRRLHAPAL
Sbjct 61 FVVLVGKPAQHGARIGAVAGAILTGDVIVSHDGELYRAVTALRQNGPRPHASRRLHAPAL 120
Query 121 CSARSRRGHLRPSCWLPPPRFAGRQSLVAR 150
CSARSRRGHLRPSCWLPPPRFAGRQSLVAR
Sbjct 121 CSARSRRGHLRPSCWLPPPRFAGRQSLVAR 150
>gi|15609080|ref|NP_216459.1| hypothetical protein Rv1943c [Mycobacterium tuberculosis H37Rv]
gi|15841414|ref|NP_336451.1| hypothetical protein MT1993 [Mycobacterium tuberculosis CDC1551]
gi|31793135|ref|NP_855628.1| hypothetical protein Mb1978c [Mycobacterium bovis AF2122/97]
81 more sequence titles
Length=125
Score = 43.9 bits (102), Expect = 0.009, Method: Compositional matrix adjust.
Identities = 20/28 (72%), Positives = 21/28 (75%), Gaps = 0/28 (0%)
Query 33 KVAVDLINLSGIQCFARIEHVAHAQAHP 60
+ AVDLIN S QCFARIEHVA QA P
Sbjct 11 RCAVDLINSSSDQCFARIEHVASDQADP 38
>gi|317501220|ref|ZP_07959425.1| cell division protein FtsW [Lachnospiraceae bacterium 8_1_57FAA]
gi|331090034|ref|ZP_08338923.1| hypothetical protein HMPREF1025_02506 [Lachnospiraceae bacterium
3_1_46FAA]
gi|336439081|ref|ZP_08618699.1| hypothetical protein HMPREF0990_01093 [Lachnospiraceae bacterium
1_1_57FAA]
gi|316897396|gb|EFV19462.1| cell division protein FtsW [Lachnospiraceae bacterium 8_1_57FAA]
gi|330402496|gb|EGG82065.1| hypothetical protein HMPREF1025_02506 [Lachnospiraceae bacterium
3_1_46FAA]
gi|336016937|gb|EGN46712.1| hypothetical protein HMPREF0990_01093 [Lachnospiraceae bacterium
1_1_57FAA]
Length=455
Score = 34.7 bits (78), Expect = 4.3, Method: Composition-based stats.
Identities = 27/82 (33%), Positives = 41/82 (50%), Gaps = 6/82 (7%)
Query 21 GSVACVQCLFLPKVAVDLINLSGIQCFARIEHVAHAQAHPFVVLVGKPAQHGARIGAVAG 80
G+VA LFL I ++GI C + V+H + PF+V++G G + AV
Sbjct 230 GAVASAGVLFLTDNLSTAIIVAGITCI--LIFVSHPKTKPFLVIIGI----GIAVAAVGI 283
Query 81 AILTGDVIVSHDGELYRAVTAL 102
AIL+ V S + L R ++ L
Sbjct 284 AILSVTVANSDNFRLQRVISWL 305
>gi|153814620|ref|ZP_01967288.1| hypothetical protein RUMTOR_00834 [Ruminococcus torques ATCC
27756]
gi|145848114|gb|EDK25032.1| hypothetical protein RUMTOR_00834 [Ruminococcus torques ATCC
27756]
Length=485
Score = 34.7 bits (78), Expect = 4.3, Method: Composition-based stats.
Identities = 27/82 (33%), Positives = 41/82 (50%), Gaps = 6/82 (7%)
Query 21 GSVACVQCLFLPKVAVDLINLSGIQCFARIEHVAHAQAHPFVVLVGKPAQHGARIGAVAG 80
G+VA LFL I ++GI C + V+H + PF+V++G G + AV
Sbjct 260 GAVASAGVLFLTDNLSTAIIVAGITCI--LIFVSHPKTKPFLVIIGI----GIAVAAVGI 313
Query 81 AILTGDVIVSHDGELYRAVTAL 102
AIL+ V S + L R ++ L
Sbjct 314 AILSVTVANSDNFRLQRVISWL 335
>gi|238061142|ref|ZP_04605851.1| diguanylate cyclase/phosphodiesterase [Micromonospora sp. ATCC
39149]
gi|237882953|gb|EEP71781.1| diguanylate cyclase/phosphodiesterase [Micromonospora sp. ATCC
39149]
Length=844
Score = 34.7 bits (78), Expect = 4.6, Method: Compositional matrix adjust.
Identities = 20/61 (33%), Positives = 31/61 (51%), Gaps = 6/61 (9%)
Query 25 CVQCLFLPKVAVDLINLSGIQCFARIEHVAHAQAHP--FVVLVGKPAQHGARIGAVAGAI 82
++ F PKV + L G++C AR EH AH P FV + A+H ++G + +
Sbjct 589 ALEVYFQPKVTLRDRRLVGVECLARWEHPAHGDVAPEDFVAV----AEHTGQLGRLTEVV 644
Query 83 L 83
L
Sbjct 645 L 645
>gi|315501454|ref|YP_004080341.1| GAF sensor-containing diguanylate cyclase/phosphodiesterase [Micromonospora
sp. L5]
gi|315408073|gb|ADU06190.1| diguanylate cyclase/phosphodiesterase with GAF sensor [Micromonospora
sp. L5]
Length=840
Score = 34.3 bits (77), Expect = 6.6, Method: Compositional matrix adjust.
Identities = 22/69 (32%), Positives = 34/69 (50%), Gaps = 6/69 (8%)
Query 26 VQCLFLPKVAVDLINLSGIQCFARIEHVAHAQAHP--FVVLVGKPAQHGARIGAVAGAIL 83
++ F PKV + L G++C AR EH AH P FV + A+H ++G + +L
Sbjct 586 LEVYFQPKVTLRDRRLVGVECLARWEHPAHGSVAPDDFVAV----AEHTGQLGRLTEFVL 641
Query 84 TGDVIVSHD 92
+ S D
Sbjct 642 RESLRRSRD 650
>gi|302869993|ref|YP_003838630.1| diguanylate cyclase [Micromonospora aurantiaca ATCC 27029]
gi|302572852|gb|ADL49054.1| diguanylate cyclase [Micromonospora aurantiaca ATCC 27029]
Length=840
Score = 34.3 bits (77), Expect = 6.7, Method: Compositional matrix adjust.
Identities = 22/69 (32%), Positives = 34/69 (50%), Gaps = 6/69 (8%)
Query 26 VQCLFLPKVAVDLINLSGIQCFARIEHVAHAQAHP--FVVLVGKPAQHGARIGAVAGAIL 83
++ F PKV + L G++C AR EH AH P FV + A+H ++G + +L
Sbjct 586 LEVYFQPKVTLRDRRLVGVECLARWEHPAHGSVAPDDFVAV----AEHTGQLGRLTEFVL 641
Query 84 TGDVIVSHD 92
+ S D
Sbjct 642 RESLRRSRD 650
Lambda K H
0.325 0.138 0.432
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 129459908798
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Sep 5, 2011 4:36 AM
Number of letters in database: 5,219,829,388
Number of sequences in database: 15,229,318
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40