BLASTP 2.2.25+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,229,318 sequences; 5,219,829,388 total letters Query= Rv2016 Length=191 Score E Sequences producing significant alignments: (Bits) Value gi|148823232|ref|YP_001287986.1| hypothetical protein TBFG_12051... 389 2e-106 gi|15609153|ref|NP_216532.1| hypothetical protein Rv2016 [Mycoba... 388 2e-106 gi|289574693|ref|ZP_06454920.1| conserved hypothetical protein [... 385 1e-105 gi|15841499|ref|NP_336536.1| hypothetical protein MT2072 [Mycoba... 385 1e-105 gi|254232187|ref|ZP_04925514.1| hypothetical protein TBCG_01969 ... 385 2e-105 gi|340627028|ref|YP_004745480.1| hypothetical protein MCAN_20391... 369 2e-100 gi|294996955|ref|ZP_06802646.1| hypothetical protein Mtub2_21223... 252 2e-65 gi|289746036|ref|ZP_06505414.1| conserved hypothetical protein [... 245 2e-63 gi|294996952|ref|ZP_06802643.1| hypothetical protein Mtub2_21208... 141 4e-32 gi|260787595|ref|XP_002588838.1| hypothetical protein BRAFLDRAFT... 35.8 2.9 gi|335049952|ref|ZP_08542933.1| NifU-like protein [Megasphaera s... 35.8 3.0 gi|290967833|ref|ZP_06559386.1| NifU-like protein [Megasphaera g... 35.4 3.7 gi|311743953|ref|ZP_07717759.1| threonine synthase [Aeromicrobiu... 34.3 8.9 >gi|148823232|ref|YP_001287986.1| hypothetical protein TBFG_12051 [Mycobacterium tuberculosis F11] gi|167970454|ref|ZP_02552731.1| hypothetical protein MtubH3_21458 [Mycobacterium tuberculosis H37Ra] gi|253798931|ref|YP_003031932.1| hypothetical protein TBMG_01969 [Mycobacterium tuberculosis KZN 1435] 18 more sequence titlesLength=203 Score = 389 bits (998), Expect = 2e-106, Method: Compositional matrix adjust. Identities = 191/191 (100%), Positives = 191/191 (100%), Gaps = 0/191 (0%) Query 1 MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS 60 MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS Sbjct 13 MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS 72 Query 61 NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEV 120 NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEV Sbjct 73 NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEV 132 Query 121 RIAVGYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD 180 RIAVGYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD Sbjct 133 RIAVGYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD 192 Query 181 LGEDSGASGER 191 LGEDSGASGER Sbjct 193 LGEDSGASGER 203 >gi|15609153|ref|NP_216532.1| hypothetical protein Rv2016 [Mycobacterium tuberculosis H37Rv] gi|31793196|ref|NP_855689.1| hypothetical protein Mb2039 [Mycobacterium bovis AF2122/97] gi|121637900|ref|YP_978123.1| hypothetical protein BCG_2033 [Mycobacterium bovis BCG str. Pasteur 1173P2] 40 more sequence titles Length=191 Score = 388 bits (997), Expect = 2e-106, Method: Compositional matrix adjust. Identities = 191/191 (100%), Positives = 191/191 (100%), Gaps = 0/191 (0%) Query 1 MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS 60 MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS Sbjct 1 MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS 60 Query 61 NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEV 120 NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEV Sbjct 61 NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEV 120 Query 121 RIAVGYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD 180 RIAVGYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD Sbjct 121 RIAVGYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD 180 Query 181 LGEDSGASGER 191 LGEDSGASGER Sbjct 181 LGEDSGASGER 191 >gi|289574693|ref|ZP_06454920.1| conserved hypothetical protein [Mycobacterium tuberculosis K85] gi|289539124|gb|EFD43702.1| conserved hypothetical protein [Mycobacterium tuberculosis K85] Length=191 Score = 385 bits (990), Expect = 1e-105, Method: Compositional matrix adjust. Identities = 190/191 (99%), Positives = 190/191 (99%), Gaps = 0/191 (0%) Query 1 MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS 60 MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS Sbjct 1 MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS 60 Query 61 NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEV 120 NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEV Sbjct 61 NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEV 120 Query 121 RIAVGYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD 180 RIAV YRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD Sbjct 121 RIAVCYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD 180 Query 181 LGEDSGASGER 191 LGEDSGASGER Sbjct 181 LGEDSGASGER 191 >gi|15841499|ref|NP_336536.1| hypothetical protein MT2072 [Mycobacterium tuberculosis CDC1551] gi|13881741|gb|AAK46350.1| hypothetical protein MT2072 [Mycobacterium tuberculosis CDC1551] Length=213 Score = 385 bits (990), Expect = 1e-105, Method: Compositional matrix adjust. Identities = 190/191 (99%), Positives = 190/191 (99%), Gaps = 0/191 (0%) Query 1 MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS 60 MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS Sbjct 23 MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS 82 Query 61 NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEV 120 NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQ AFPGLEEV Sbjct 83 NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQLAFPGLEEV 142 Query 121 RIAVGYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD 180 RIAVGYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD Sbjct 143 RIAVGYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD 202 Query 181 LGEDSGASGER 191 LGEDSGASGER Sbjct 203 LGEDSGASGER 213 >gi|254232187|ref|ZP_04925514.1| hypothetical protein TBCG_01969 [Mycobacterium tuberculosis C] gi|254364834|ref|ZP_04980880.1| hypothetical protein TBHG_01973 [Mycobacterium tuberculosis str. Haarlem] gi|124601246|gb|EAY60256.1| hypothetical protein TBCG_01969 [Mycobacterium tuberculosis C] gi|134150348|gb|EBA42393.1| hypothetical protein TBHG_01973 [Mycobacterium tuberculosis str. Haarlem] gi|323719419|gb|EGB28547.1| hypothetical protein TMMG_01284 [Mycobacterium tuberculosis CDC1551A] Length=203 Score = 385 bits (989), Expect = 2e-105, Method: Compositional matrix adjust. Identities = 190/191 (99%), Positives = 190/191 (99%), Gaps = 0/191 (0%) Query 1 MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS 60 MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS Sbjct 13 MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS 72 Query 61 NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEV 120 NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQ AFPGLEEV Sbjct 73 NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQLAFPGLEEV 132 Query 121 RIAVGYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD 180 RIAVGYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD Sbjct 133 RIAVGYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD 192 Query 181 LGEDSGASGER 191 LGEDSGASGER Sbjct 193 LGEDSGASGER 203 >gi|340627028|ref|YP_004745480.1| hypothetical protein MCAN_20391 [Mycobacterium canettii CIPT 140010059] gi|340005218|emb|CCC44371.1| hypothetical protein MCAN_20391 [Mycobacterium canettii CIPT 140010059] Length=191 Score = 369 bits (946), Expect = 2e-100, Method: Compositional matrix adjust. Identities = 180/191 (95%), Positives = 186/191 (98%), Gaps = 0/191 (0%) Query 1 MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS 60 MTELGDK LAALV IRDTR DIADMR WRPGWFPTMHSRCLS+LIHDRIWAHLVTLIAS Sbjct 1 MTELGDKLLAALVSAIRDTRVDIADMREWRPGWFPTMHSRCLSSLIHDRIWAHLVTLIAS 60 Query 61 NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEV 120 +PGT+IK+KGATREIVVGAHLRLRIKRHHAGDEISTYPT+TAIEFWQQGSQPAFPGLEEV Sbjct 61 DPGTNIKEKGATREIVVGAHLRLRIKRHHAGDEISTYPTQTAIEFWQQGSQPAFPGLEEV 120 Query 121 RIAVGYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD 180 RIAVGYRWDPDTR+IGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD Sbjct 121 RIAVGYRWDPDTRDIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD 180 Query 181 LGEDSGASGER 191 LGEDSGASGER Sbjct 181 LGEDSGASGER 191 >gi|294996955|ref|ZP_06802646.1| hypothetical protein Mtub2_21223 [Mycobacterium tuberculosis 210] gi|339294943|gb|AEJ47054.1| hypothetical protein CCDC5079_1864 [Mycobacterium tuberculosis CCDC5079] gi|339298566|gb|AEJ50676.1| hypothetical protein CCDC5180_1839 [Mycobacterium tuberculosis CCDC5180] Length=130 Score = 252 bits (643), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 125/126 (99%), Positives = 125/126 (99%), Gaps = 0/126 (0%) Query 66 IKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEVRIAVG 125 KDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEVRIAVG Sbjct 5 FKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEVRIAVG 64 Query 126 YRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGDLGEDS 185 YRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGDLGEDS Sbjct 65 YRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGDLGEDS 124 Query 186 GASGER 191 GASGER Sbjct 125 GASGER 130 >gi|289746036|ref|ZP_06505414.1| conserved hypothetical protein [Mycobacterium tuberculosis 02_1987] gi|289686564|gb|EFD54052.1| conserved hypothetical protein [Mycobacterium tuberculosis 02_1987] Length=134 Score = 245 bits (625), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 118/119 (99%), Positives = 119/119 (100%), Gaps = 0/119 (0%) Query 1 MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS 60 MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS Sbjct 13 MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS 72 Query 61 NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEE 119 NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGL+E Sbjct 73 NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLDE 131 >gi|294996952|ref|ZP_06802643.1| hypothetical protein Mtub2_21208 [Mycobacterium tuberculosis 210] Length=79 Score = 141 bits (356), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 67/67 (100%), Positives = 67/67 (100%), Gaps = 0/67 (0%) Query 1 MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS 60 MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS Sbjct 1 MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS 60 Query 61 NPGTSIK 67 NPGTSIK Sbjct 61 NPGTSIK 67 >gi|260787595|ref|XP_002588838.1| hypothetical protein BRAFLDRAFT_99538 [Branchiostoma floridae] gi|229274008|gb|EEN44849.1| hypothetical protein BRAFLDRAFT_99538 [Branchiostoma floridae] Length=1243 Score = 35.8 bits (81), Expect = 2.9, Method: Composition-based stats. Identities = 22/64 (35%), Positives = 34/64 (54%), Gaps = 3/64 (4%) Query 117 LEEVRIAVGYRW--DPDTREIGAPLLSLRDGKDHV-IWVVELDEPAAGVKITWTPIEPTL 173 L +R+ +G W DPDTR +G +L+ +H + VVELD P + + T +E Sbjct 1005 LRSLRLKIGEWWRADPDTRGLGNLTXTLQSISEHTKLEVVELDFPRNDISVEHTGVEVLR 1064 Query 174 PSID 177 +ID Sbjct 1065 NTID 1068 >gi|335049952|ref|ZP_08542933.1| NifU-like protein [Megasphaera sp. UPII 199-6] gi|333761859|gb|EGL39385.1| NifU-like protein [Megasphaera sp. UPII 199-6] Length=94 Score = 35.8 bits (81), Expect = 3.0, Method: Compositional matrix adjust. Identities = 27/76 (36%), Positives = 37/76 (49%), Gaps = 5/76 (6%) Query 52 AHLVTLIASNPGTSIKDKGATREIV--VGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQG 109 A L TL+A S++ G EI+ LR+R+ +G +T T EF Q Sbjct 5 AQLETLLAEKIRPSLQAHGGNVEIISYTDGILRIRLTGRCSGCPSATLTTE---EFINQI 61 Query 110 SQPAFPGLEEVRIAVG 125 Q AFP + EVR+A G Sbjct 62 VQTAFPDVREVRLAAG 77 >gi|290967833|ref|ZP_06559386.1| NifU-like protein [Megasphaera genomosp. type_1 str. 28L] gi|290782192|gb|EFD94767.1| NifU-like protein [Megasphaera genomosp. type_1 str. 28L] Length=94 Score = 35.4 bits (80), Expect = 3.7, Method: Compositional matrix adjust. Identities = 27/76 (36%), Positives = 37/76 (49%), Gaps = 5/76 (6%) Query 52 AHLVTLIASNPGTSIKDKGATREIV--VGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQG 109 A L TL+A S++ G EI+ LR+R+ +G +T T EF Q Sbjct 5 AQLETLLAEKIRPSLQAHGGNVEIISYTDGILRIRLTGRCSGCPSATLTTE---EFINQI 61 Query 110 SQPAFPGLEEVRIAVG 125 Q AFP + EVR+A G Sbjct 62 VQTAFPDVREVRLAAG 77 >gi|311743953|ref|ZP_07717759.1| threonine synthase [Aeromicrobium marinum DSM 15272] gi|311313083|gb|EFQ82994.1| threonine synthase [Aeromicrobium marinum DSM 15272] Length=363 Score = 34.3 bits (77), Expect = 8.9, Method: Compositional matrix adjust. Identities = 23/78 (30%), Positives = 34/78 (44%), Gaps = 7/78 (8%) Query 30 RPGWFPTMHSRCLSNLIHDRIWAHLVTLIASNPGTSIKDKGATREIVV----GAHLRLRI 85 R G P +HS LS L+H +W + + NP S KD+G T I V GA + Sbjct 33 REGGTPLVHSAWLSGLVHGDVW---LKVEGDNPTGSFKDRGMTAAISVAVGEGAKAVVCA 89 Query 86 KRHHAGDEISTYPTRTAI 103 + ++ Y R + Sbjct 90 STGNTSASMTAYAARAGL 107 Lambda K H 0.320 0.138 0.443 Gapped Lambda K H 0.267 0.0410 0.140 Effective search space used: 189364005308 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects Posted date: Sep 5, 2011 4:36 AM Number of letters in database: 5,219,829,388 Number of sequences in database: 15,229,318 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 11 Window for multiple hits: 40