BLASTP 2.2.25+


Reference:
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database
search programs", Nucleic Acids Res. 25:3389-3402.



Reference for composition-based statistics:
Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.



Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
           15,229,318 sequences; 5,219,829,388 total letters



Query= Rv2016

Length=191
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

gi|148823232|ref|YP_001287986.1|  hypothetical protein TBFG_12051...   389    2e-106
gi|15609153|ref|NP_216532.1|  hypothetical protein Rv2016 [Mycoba...   388    2e-106
gi|289574693|ref|ZP_06454920.1|  conserved hypothetical protein [...   385    1e-105
gi|15841499|ref|NP_336536.1|  hypothetical protein MT2072 [Mycoba...   385    1e-105
gi|254232187|ref|ZP_04925514.1|  hypothetical protein TBCG_01969 ...   385    2e-105
gi|340627028|ref|YP_004745480.1|  hypothetical protein MCAN_20391...   369    2e-100
gi|294996955|ref|ZP_06802646.1|  hypothetical protein Mtub2_21223...   252    2e-65 
gi|289746036|ref|ZP_06505414.1|  conserved hypothetical protein [...   245    2e-63 
gi|294996952|ref|ZP_06802643.1|  hypothetical protein Mtub2_21208...   141    4e-32 
gi|260787595|ref|XP_002588838.1|  hypothetical protein BRAFLDRAFT...  35.8    2.9   
gi|335049952|ref|ZP_08542933.1|  NifU-like protein [Megasphaera s...  35.8    3.0   
gi|290967833|ref|ZP_06559386.1|  NifU-like protein [Megasphaera g...  35.4    3.7   
gi|311743953|ref|ZP_07717759.1|  threonine synthase [Aeromicrobiu...  34.3    8.9   


>gi|148823232|ref|YP_001287986.1| hypothetical protein TBFG_12051 [Mycobacterium tuberculosis F11]
 gi|167970454|ref|ZP_02552731.1| hypothetical protein MtubH3_21458 [Mycobacterium tuberculosis 
H37Ra]
 gi|253798931|ref|YP_003031932.1| hypothetical protein TBMG_01969 [Mycobacterium tuberculosis KZN 
1435]
 18 more sequence titles
 Length=203

 Score =  389 bits (998),  Expect = 2e-106, Method: Compositional matrix adjust.
 Identities = 191/191 (100%), Positives = 191/191 (100%), Gaps = 0/191 (0%)

Query  1    MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS  60
            MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS
Sbjct  13   MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS  72

Query  61   NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEV  120
            NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEV
Sbjct  73   NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEV  132

Query  121  RIAVGYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD  180
            RIAVGYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD
Sbjct  133  RIAVGYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD  192

Query  181  LGEDSGASGER  191
            LGEDSGASGER
Sbjct  193  LGEDSGASGER  203


>gi|15609153|ref|NP_216532.1| hypothetical protein Rv2016 [Mycobacterium tuberculosis H37Rv]
 gi|31793196|ref|NP_855689.1| hypothetical protein Mb2039 [Mycobacterium bovis AF2122/97]
 gi|121637900|ref|YP_978123.1| hypothetical protein BCG_2033 [Mycobacterium bovis BCG str. Pasteur 
1173P2]
 40 more sequence titles
 Length=191

 Score =  388 bits (997),  Expect = 2e-106, Method: Compositional matrix adjust.
 Identities = 191/191 (100%), Positives = 191/191 (100%), Gaps = 0/191 (0%)

Query  1    MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS  60
            MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS
Sbjct  1    MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS  60

Query  61   NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEV  120
            NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEV
Sbjct  61   NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEV  120

Query  121  RIAVGYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD  180
            RIAVGYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD
Sbjct  121  RIAVGYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD  180

Query  181  LGEDSGASGER  191
            LGEDSGASGER
Sbjct  181  LGEDSGASGER  191


>gi|289574693|ref|ZP_06454920.1| conserved hypothetical protein [Mycobacterium tuberculosis K85]
 gi|289539124|gb|EFD43702.1| conserved hypothetical protein [Mycobacterium tuberculosis K85]
Length=191

 Score =  385 bits (990),  Expect = 1e-105, Method: Compositional matrix adjust.
 Identities = 190/191 (99%), Positives = 190/191 (99%), Gaps = 0/191 (0%)

Query  1    MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS  60
            MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS
Sbjct  1    MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS  60

Query  61   NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEV  120
            NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEV
Sbjct  61   NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEV  120

Query  121  RIAVGYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD  180
            RIAV YRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD
Sbjct  121  RIAVCYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD  180

Query  181  LGEDSGASGER  191
            LGEDSGASGER
Sbjct  181  LGEDSGASGER  191


>gi|15841499|ref|NP_336536.1| hypothetical protein MT2072 [Mycobacterium tuberculosis CDC1551]
 gi|13881741|gb|AAK46350.1| hypothetical protein MT2072 [Mycobacterium tuberculosis CDC1551]
Length=213

 Score =  385 bits (990),  Expect = 1e-105, Method: Compositional matrix adjust.
 Identities = 190/191 (99%), Positives = 190/191 (99%), Gaps = 0/191 (0%)

Query  1    MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS  60
            MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS
Sbjct  23   MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS  82

Query  61   NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEV  120
            NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQ AFPGLEEV
Sbjct  83   NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQLAFPGLEEV  142

Query  121  RIAVGYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD  180
            RIAVGYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD
Sbjct  143  RIAVGYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD  202

Query  181  LGEDSGASGER  191
            LGEDSGASGER
Sbjct  203  LGEDSGASGER  213


>gi|254232187|ref|ZP_04925514.1| hypothetical protein TBCG_01969 [Mycobacterium tuberculosis C]
 gi|254364834|ref|ZP_04980880.1| hypothetical protein TBHG_01973 [Mycobacterium tuberculosis str. 
Haarlem]
 gi|124601246|gb|EAY60256.1| hypothetical protein TBCG_01969 [Mycobacterium tuberculosis C]
 gi|134150348|gb|EBA42393.1| hypothetical protein TBHG_01973 [Mycobacterium tuberculosis str. 
Haarlem]
 gi|323719419|gb|EGB28547.1| hypothetical protein TMMG_01284 [Mycobacterium tuberculosis CDC1551A]
Length=203

 Score =  385 bits (989),  Expect = 2e-105, Method: Compositional matrix adjust.
 Identities = 190/191 (99%), Positives = 190/191 (99%), Gaps = 0/191 (0%)

Query  1    MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS  60
            MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS
Sbjct  13   MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS  72

Query  61   NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEV  120
            NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQ AFPGLEEV
Sbjct  73   NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQLAFPGLEEV  132

Query  121  RIAVGYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD  180
            RIAVGYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD
Sbjct  133  RIAVGYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD  192

Query  181  LGEDSGASGER  191
            LGEDSGASGER
Sbjct  193  LGEDSGASGER  203


>gi|340627028|ref|YP_004745480.1| hypothetical protein MCAN_20391 [Mycobacterium canettii CIPT 
140010059]
 gi|340005218|emb|CCC44371.1| hypothetical protein MCAN_20391 [Mycobacterium canettii CIPT 
140010059]
Length=191

 Score =  369 bits (946),  Expect = 2e-100, Method: Compositional matrix adjust.
 Identities = 180/191 (95%), Positives = 186/191 (98%), Gaps = 0/191 (0%)

Query  1    MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS  60
            MTELGDK LAALV  IRDTR DIADMR WRPGWFPTMHSRCLS+LIHDRIWAHLVTLIAS
Sbjct  1    MTELGDKLLAALVSAIRDTRVDIADMREWRPGWFPTMHSRCLSSLIHDRIWAHLVTLIAS  60

Query  61   NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEV  120
            +PGT+IK+KGATREIVVGAHLRLRIKRHHAGDEISTYPT+TAIEFWQQGSQPAFPGLEEV
Sbjct  61   DPGTNIKEKGATREIVVGAHLRLRIKRHHAGDEISTYPTQTAIEFWQQGSQPAFPGLEEV  120

Query  121  RIAVGYRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD  180
            RIAVGYRWDPDTR+IGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD
Sbjct  121  RIAVGYRWDPDTRDIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGD  180

Query  181  LGEDSGASGER  191
            LGEDSGASGER
Sbjct  181  LGEDSGASGER  191


>gi|294996955|ref|ZP_06802646.1| hypothetical protein Mtub2_21223 [Mycobacterium tuberculosis 
210]
 gi|339294943|gb|AEJ47054.1| hypothetical protein CCDC5079_1864 [Mycobacterium tuberculosis 
CCDC5079]
 gi|339298566|gb|AEJ50676.1| hypothetical protein CCDC5180_1839 [Mycobacterium tuberculosis 
CCDC5180]
Length=130

 Score =  252 bits (643),  Expect = 2e-65, Method: Compositional matrix adjust.
 Identities = 125/126 (99%), Positives = 125/126 (99%), Gaps = 0/126 (0%)

Query  66   IKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEVRIAVG  125
             KDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEVRIAVG
Sbjct  5    FKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEEVRIAVG  64

Query  126  YRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGDLGEDS  185
            YRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGDLGEDS
Sbjct  65   YRWDPDTREIGAPLLSLRDGKDHVIWVVELDEPAAGVKITWTPIEPTLPSIDFGDLGEDS  124

Query  186  GASGER  191
            GASGER
Sbjct  125  GASGER  130


>gi|289746036|ref|ZP_06505414.1| conserved hypothetical protein [Mycobacterium tuberculosis 02_1987]
 gi|289686564|gb|EFD54052.1| conserved hypothetical protein [Mycobacterium tuberculosis 02_1987]
Length=134

 Score =  245 bits (625),  Expect = 2e-63, Method: Compositional matrix adjust.
 Identities = 118/119 (99%), Positives = 119/119 (100%), Gaps = 0/119 (0%)

Query  1    MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS  60
            MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS
Sbjct  13   MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS  72

Query  61   NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLEE  119
            NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGL+E
Sbjct  73   NPGTSIKDKGATREIVVGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQGSQPAFPGLDE  131


>gi|294996952|ref|ZP_06802643.1| hypothetical protein Mtub2_21208 [Mycobacterium tuberculosis 
210]
Length=79

 Score =  141 bits (356),  Expect = 4e-32, Method: Compositional matrix adjust.
 Identities = 67/67 (100%), Positives = 67/67 (100%), Gaps = 0/67 (0%)

Query  1   MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS  60
           MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS
Sbjct  1   MTELGDKFLAALVGTIRDTRFDIADMRNWRPGWFPTMHSRCLSNLIHDRIWAHLVTLIAS  60

Query  61  NPGTSIK  67
           NPGTSIK
Sbjct  61  NPGTSIK  67


>gi|260787595|ref|XP_002588838.1| hypothetical protein BRAFLDRAFT_99538 [Branchiostoma floridae]
 gi|229274008|gb|EEN44849.1| hypothetical protein BRAFLDRAFT_99538 [Branchiostoma floridae]
Length=1243

 Score = 35.8 bits (81),  Expect = 2.9, Method: Composition-based stats.
 Identities = 22/64 (35%), Positives = 34/64 (54%), Gaps = 3/64 (4%)

Query  117   LEEVRIAVGYRW--DPDTREIGAPLLSLRDGKDHV-IWVVELDEPAAGVKITWTPIEPTL  173
             L  +R+ +G  W  DPDTR +G    +L+   +H  + VVELD P   + +  T +E   
Sbjct  1005  LRSLRLKIGEWWRADPDTRGLGNLTXTLQSISEHTKLEVVELDFPRNDISVEHTGVEVLR  1064

Query  174   PSID  177
              +ID
Sbjct  1065  NTID  1068


>gi|335049952|ref|ZP_08542933.1| NifU-like protein [Megasphaera sp. UPII 199-6]
 gi|333761859|gb|EGL39385.1| NifU-like protein [Megasphaera sp. UPII 199-6]
Length=94

 Score = 35.8 bits (81),  Expect = 3.0, Method: Compositional matrix adjust.
 Identities = 27/76 (36%), Positives = 37/76 (49%), Gaps = 5/76 (6%)

Query  52   AHLVTLIASNPGTSIKDKGATREIV--VGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQG  109
            A L TL+A     S++  G   EI+      LR+R+    +G   +T  T    EF  Q 
Sbjct  5    AQLETLLAEKIRPSLQAHGGNVEIISYTDGILRIRLTGRCSGCPSATLTTE---EFINQI  61

Query  110  SQPAFPGLEEVRIAVG  125
             Q AFP + EVR+A G
Sbjct  62   VQTAFPDVREVRLAAG  77


>gi|290967833|ref|ZP_06559386.1| NifU-like protein [Megasphaera genomosp. type_1 str. 28L]
 gi|290782192|gb|EFD94767.1| NifU-like protein [Megasphaera genomosp. type_1 str. 28L]
Length=94

 Score = 35.4 bits (80),  Expect = 3.7, Method: Compositional matrix adjust.
 Identities = 27/76 (36%), Positives = 37/76 (49%), Gaps = 5/76 (6%)

Query  52   AHLVTLIASNPGTSIKDKGATREIV--VGAHLRLRIKRHHAGDEISTYPTRTAIEFWQQG  109
            A L TL+A     S++  G   EI+      LR+R+    +G   +T  T    EF  Q 
Sbjct  5    AQLETLLAEKIRPSLQAHGGNVEIISYTDGILRIRLTGRCSGCPSATLTTE---EFINQI  61

Query  110  SQPAFPGLEEVRIAVG  125
             Q AFP + EVR+A G
Sbjct  62   VQTAFPDVREVRLAAG  77


>gi|311743953|ref|ZP_07717759.1| threonine synthase [Aeromicrobium marinum DSM 15272]
 gi|311313083|gb|EFQ82994.1| threonine synthase [Aeromicrobium marinum DSM 15272]
Length=363

 Score = 34.3 bits (77),  Expect = 8.9, Method: Compositional matrix adjust.
 Identities = 23/78 (30%), Positives = 34/78 (44%), Gaps = 7/78 (8%)

Query  30   RPGWFPTMHSRCLSNLIHDRIWAHLVTLIASNPGTSIKDKGATREIVV----GAHLRLRI  85
            R G  P +HS  LS L+H  +W   + +   NP  S KD+G T  I V    GA   +  
Sbjct  33   REGGTPLVHSAWLSGLVHGDVW---LKVEGDNPTGSFKDRGMTAAISVAVGEGAKAVVCA  89

Query  86   KRHHAGDEISTYPTRTAI  103
               +    ++ Y  R  +
Sbjct  90   STGNTSASMTAYAARAGL  107



Lambda     K      H
   0.320    0.138    0.443 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Effective search space used: 189364005308


  Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
    Posted date:  Sep 5, 2011  4:36 AM
  Number of letters in database: 5,219,829,388
  Number of sequences in database:  15,229,318



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40