BLASTP 2.2.25+


Reference:
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database
search programs", Nucleic Acids Res. 25:3389-3402.



Reference for composition-based statistics:
Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.



Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
           15,229,318 sequences; 5,219,829,388 total letters



Query= Rv2054

Length=237
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

gi|15609191|ref|NP_216570.1|  hypothetical protein Rv2054 [Mycoba...   478    2e-133
gi|289447674|ref|ZP_06437418.1|  conserved hypothetical protein [...   477    7e-133
gi|339632094|ref|YP_004723736.1|  hypothetical protein MAF_20690 ...   477    8e-133
gi|340627065|ref|YP_004745517.1|  hypothetical protein MCAN_20771...   476    1e-132
gi|183983033|ref|YP_001851324.1|  hypothetical protein MMAR_3032 ...   413    1e-113
gi|118617792|ref|YP_906124.1|  hypothetical protein MUL_2272 [Myc...   413    1e-113
gi|240173215|ref|ZP_04751873.1|  hypothetical protein MkanA1_2813...   409    2e-112
gi|296165146|ref|ZP_06847695.1|  dienelactone hydrolase family pr...   377    8e-103
gi|333990745|ref|YP_004523359.1|  hypothetical protein JDM601_210...   377    1e-102
gi|342859769|ref|ZP_08716422.1|  hypothetical protein MCOL_12858 ...   375    3e-102
gi|15827757|ref|NP_302020.1|  dienelactone hydrolase [Mycobacteri...   370    9e-101
gi|254775104|ref|ZP_05216620.1|  hypothetical protein MaviaA2_105...   367    6e-100
gi|118472341|ref|YP_886784.1|  dienelactone hydrolase [Mycobacter...   365    3e-99 
gi|41407901|ref|NP_960737.1|  hypothetical protein MAP1803 [Mycob...   362    2e-98 
gi|254819614|ref|ZP_05224615.1|  hypothetical protein MintA_06804...   362    3e-98 
gi|126435120|ref|YP_001070811.1|  dienelactone hydrolase [Mycobac...   320    8e-86 
gi|108799466|ref|YP_639663.1|  dienelactone hydrolase [Mycobacter...   320    1e-85 
gi|118467084|ref|YP_881637.1|  dienelactone hydrolase [Mycobacter...   315    5e-84 
gi|333918339|ref|YP_004491920.1|  hypothetical protein AS9A_0666 ...   276    2e-72 
gi|333919407|ref|YP_004492988.1|  hypothetical protein AS9A_1739 ...   269    2e-70 
gi|312138239|ref|YP_004005575.1|  carboxymethylenebutenolidase [R...   269    3e-70 
gi|325674853|ref|ZP_08154540.1|  dienelactone hydrolase [Rhodococ...   268    5e-70 
gi|134101850|ref|YP_001107511.1|  putative carboxymethylenebuteno...   245    3e-63 
gi|300790960|ref|YP_003771251.1|  carboxymethylenebutenolidase [A...   233    1e-59 
gi|326332773|ref|ZP_08199034.1|  putative carboxymethylenebutenol...   230    1e-58 
gi|54024910|ref|YP_119152.1|  putative hydrolase [Nocardia farcin...   223    2e-56 
gi|296393433|ref|YP_003658317.1|  dienelactone hydrolase [Segnili...   212    4e-53 
gi|317506550|ref|ZP_07964345.1|  dienelactone hydrolase [Segnilip...   210    1e-52 
gi|148272701|ref|YP_001222262.1|  putative carboxymethylenebuteno...   199    3e-49 
gi|312199662|ref|YP_004019723.1|  carboxymethylenebutenolidase [F...   191    1e-46 
gi|170782159|ref|YP_001710492.1|  putative hydrolase [Clavibacter...   190    2e-46 
gi|118465396|ref|YP_879769.1|  dienelactone hydrolase [Mycobacter...   189    4e-46 
gi|111019264|ref|YP_702236.1|  carboxymethylenebutenolidase [Rhod...   186    3e-45 
gi|297163413|gb|ADI13125.1|  putative carboxymethylenebutenolidas...   186    4e-45 
gi|226361394|ref|YP_002779172.1|  carboxymethylenebutenolidase [R...   183    2e-44 
gi|156742170|ref|YP_001432299.1|  dienelactone hydrolase [Roseifl...   182    4e-44 
gi|148656803|ref|YP_001277008.1|  dienelactone hydrolase [Roseifl...   179    4e-43 
gi|226308781|ref|YP_002768741.1|  carboxymethylenebutenolidase [R...   177    9e-43 
gi|111224276|ref|YP_715070.1|  putative carboxymethylenebutenolid...   176    2e-42 
gi|229489128|ref|ZP_04382994.1|  dienelactone hydrolase family pr...   171    7e-41 
gi|163841096|ref|YP_001625501.1|  carboxymethylenebutenolidase [R...   164    1e-38 
gi|258653114|ref|YP_003202270.1|  dienelactone hydrolase [Nakamur...   163    2e-38 
gi|116671815|ref|YP_832748.1|  dienelactone hydrolase [Arthrobact...   154    1e-35 
gi|262200320|ref|YP_003271528.1|  dienelactone hydrolase [Gordoni...   144    1e-32 
gi|220913809|ref|YP_002489118.1|  dienelactone hydrolase [Arthrob...   141    6e-32 
gi|325001844|ref|ZP_08122956.1|  carboxymethylenebutenolidase [Ps...   135    4e-30 
gi|184201845|ref|YP_001856052.1|  hypothetical protein KRH_21990 ...   131    9e-29 
gi|336117290|ref|YP_004572057.1|  putative hydrolase [Microlunatu...   129    5e-28 
gi|254429403|ref|ZP_05043110.1|  dienelactone hydrolase family [A...   123    2e-26 
gi|110834479|ref|YP_693338.1|  dienelactone hydrolase family prot...   123    3e-26 


>gi|15609191|ref|NP_216570.1| hypothetical protein Rv2054 [Mycobacterium tuberculosis H37Rv]
 gi|15841542|ref|NP_336579.1| hypothetical protein MT2114 [Mycobacterium tuberculosis CDC1551]
 gi|31793237|ref|NP_855730.1| hypothetical protein Mb2080 [Mycobacterium bovis AF2122/97]
 72 more sequence titles
 Length=237

 Score =  478 bits (1231),  Expect = 2e-133, Method: Compositional matrix adjust.
 Identities = 237/237 (100%), Positives = 237/237 (100%), Gaps = 0/237 (0%)

Query  1    MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN  60
            MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN
Sbjct  1    MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN  60

Query  61   MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALV  120
            MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALV
Sbjct  61   MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALV  120

Query  121  LSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPA  180
            LSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPA
Sbjct  121  LSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPA  180

Query  181  DIKSYPGAGHSFANKLPGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLRAGSPGEP  237
            DIKSYPGAGHSFANKLPGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLRAGSPGEP
Sbjct  181  DIKSYPGAGHSFANKLPGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLRAGSPGEP  237


>gi|289447674|ref|ZP_06437418.1| conserved hypothetical protein [Mycobacterium tuberculosis CPHL_A]
 gi|289420632|gb|EFD17833.1| conserved hypothetical protein [Mycobacterium tuberculosis CPHL_A]
Length=237

 Score =  477 bits (1227),  Expect = 7e-133, Method: Compositional matrix adjust.
 Identities = 236/237 (99%), Positives = 236/237 (99%), Gaps = 0/237 (0%)

Query  1    MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN  60
            MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN
Sbjct  1    MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN  60

Query  61   MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALV  120
            MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALV
Sbjct  61   MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALV  120

Query  121  LSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPA  180
            LSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPA
Sbjct  121  LSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPA  180

Query  181  DIKSYPGAGHSFANKLPGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLRAGSPGEP  237
            DIKSYPGAGHSFANKLPGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLR GSPGEP
Sbjct  181  DIKSYPGAGHSFANKLPGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLRTGSPGEP  237


>gi|339632094|ref|YP_004723736.1| hypothetical protein MAF_20690 [Mycobacterium africanum GM041182]
 gi|339331450|emb|CCC27139.1| conserved hypothetical protein [Mycobacterium africanum GM041182]
Length=237

 Score =  477 bits (1227),  Expect = 8e-133, Method: Compositional matrix adjust.
 Identities = 236/237 (99%), Positives = 236/237 (99%), Gaps = 0/237 (0%)

Query  1    MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN  60
            MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN
Sbjct  1    MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN  60

Query  61   MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALV  120
            MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALV
Sbjct  61   MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALV  120

Query  121  LSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPA  180
            LSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPA
Sbjct  121  LSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPA  180

Query  181  DIKSYPGAGHSFANKLPGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLRAGSPGEP  237
            DIKSYPGAGHSFANKLPGQPLVRIAGFGYNEA TEDAWRRVFEFFGQHLRAGSPGEP
Sbjct  181  DIKSYPGAGHSFANKLPGQPLVRIAGFGYNEAVTEDAWRRVFEFFGQHLRAGSPGEP  237


>gi|340627065|ref|YP_004745517.1| hypothetical protein MCAN_20771 [Mycobacterium canettii CIPT 
140010059]
 gi|340005255|emb|CCC44409.1| conserved hypothetical protein [Mycobacterium canettii CIPT 140010059]
Length=237

 Score =  476 bits (1224),  Expect = 1e-132, Method: Compositional matrix adjust.
 Identities = 236/237 (99%), Positives = 236/237 (99%), Gaps = 0/237 (0%)

Query  1    MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN  60
            MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN
Sbjct  1    MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN  60

Query  61   MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALV  120
            MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALV
Sbjct  61   MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALV  120

Query  121  LSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPA  180
            LSPRGFGATAPFYGTPLPRHLSETLN ACPIVASFGTRDPLGIGAANRLRKVTAAKNIPA
Sbjct  121  LSPRGFGATAPFYGTPLPRHLSETLNEACPIVASFGTRDPLGIGAANRLRKVTAAKNIPA  180

Query  181  DIKSYPGAGHSFANKLPGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLRAGSPGEP  237
            DIKSYPGAGHSFANKLPGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLRAGSPGEP
Sbjct  181  DIKSYPGAGHSFANKLPGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLRAGSPGEP  237


>gi|183983033|ref|YP_001851324.1| hypothetical protein MMAR_3032 [Mycobacterium marinum M]
 gi|183176359|gb|ACC41469.1| conserved hypothetical protein [Mycobacterium marinum M]
Length=260

 Score =  413 bits (1062),  Expect = 1e-113, Method: Compositional matrix adjust.
 Identities = 199/233 (86%), Positives = 215/233 (93%), Gaps = 0/233 (0%)

Query  1    MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN  60
            MTTI+ID PAGPIDALLG+P G+GPWPGVVVVHDA+GY PDN+ ISERIA AGY+ LTPN
Sbjct  25   MTTIDIDTPAGPIDALLGVPTGEGPWPGVVVVHDAIGYAPDNEAISERIAAAGYLALTPN  84

Query  61   MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALV  120
            MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAM ECSG+VGI GFCMGGQFAL+
Sbjct  85   MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMAECSGQVGIAGFCMGGQFALI  144

Query  121  LSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPA  180
            LSPRGFGA+APFYGTPLPRHLSETL+GACPIVASFG RDPLG+GA +RLR+VT AK IPA
Sbjct  145  LSPRGFGASAPFYGTPLPRHLSETLDGACPIVASFGNRDPLGLGAPSRLREVTQAKQIPA  204

Query  181  DIKSYPGAGHSFANKLPGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLRAGS  233
            DIK YPGAGHSFANKLP QPL+RI GFGYNEAATEDAW RVF FFG+HL AG+
Sbjct  205  DIKGYPGAGHSFANKLPRQPLLRITGFGYNEAATEDAWGRVFAFFGEHLAAGT  257


>gi|118617792|ref|YP_906124.1| hypothetical protein MUL_2272 [Mycobacterium ulcerans Agy99]
 gi|118569902|gb|ABL04653.1| conserved hypothetical protein [Mycobacterium ulcerans Agy99]
Length=236

 Score =  413 bits (1061),  Expect = 1e-113, Method: Compositional matrix adjust.
 Identities = 199/236 (85%), Positives = 216/236 (92%), Gaps = 0/236 (0%)

Query  1    MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN  60
            MTTI+ID PAGPIDALLG+P G+GPWPGVVVVHDA+GY PDN+ ISERIA AGY+ LTPN
Sbjct  1    MTTIDIDTPAGPIDALLGVPTGEGPWPGVVVVHDAIGYAPDNEAISERIAAAGYLALTPN  60

Query  61   MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALV  120
            MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAM ECSG+VGI GFCMGGQFAL+
Sbjct  61   MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMAECSGQVGIAGFCMGGQFALI  120

Query  121  LSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPA  180
            LSPRGFGA+APFYGTPLPRHLSETL+GACPIVASFG RDPLG+GA +RLR+VT AK IPA
Sbjct  121  LSPRGFGASAPFYGTPLPRHLSETLDGACPIVASFGNRDPLGLGAPSRLREVTQAKQIPA  180

Query  181  DIKSYPGAGHSFANKLPGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLRAGSPGE  236
            DI+ YPGAGHSFANKLP QPL+RI GFGYNEAATEDAW RVF FFG+HL AG+ G 
Sbjct  181  DIEGYPGAGHSFANKLPRQPLLRITGFGYNEAATEDAWGRVFAFFGEHLAAGTSGN  236


>gi|240173215|ref|ZP_04751873.1| hypothetical protein MkanA1_28131 [Mycobacterium kansasii ATCC 
12478]
Length=233

 Score =  409 bits (1050),  Expect = 2e-112, Method: Compositional matrix adjust.
 Identities = 196/233 (85%), Positives = 214/233 (92%), Gaps = 0/233 (0%)

Query  1    MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN  60
            MTTI+ID PAG IDALL +P G+GPWPGVVVVHDAVGY PDN+ IS+RIA+AGY+ LTPN
Sbjct  1    MTTIDIDTPAGTIDALLSVPTGEGPWPGVVVVHDAVGYAPDNEAISQRIAQAGYMALTPN  60

Query  61   MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALV  120
            MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAM ECSGRVGI GFCMGG FAL+
Sbjct  61   MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMAECSGRVGIAGFCMGGGFALI  120

Query  121  LSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPA  180
            LSPRGFGA+APFYGTPLPRHLS+TL+GACPIVASFG RDPLG+GAA+RLR VT AK I A
Sbjct  121  LSPRGFGASAPFYGTPLPRHLSDTLDGACPIVASFGGRDPLGLGAADRLRSVTQAKGITA  180

Query  181  DIKSYPGAGHSFANKLPGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLRAGS  233
            DIK YPGAGHSFANKLPGQPL+RI+GFGYNE ATEDAWRRVF FFG+HL +G+
Sbjct  181  DIKVYPGAGHSFANKLPGQPLIRISGFGYNETATEDAWRRVFAFFGEHLGSGT  233


>gi|296165146|ref|ZP_06847695.1| dienelactone hydrolase family protein [Mycobacterium parascrofulaceum 
ATCC BAA-614]
 gi|295899490|gb|EFG78947.1| dienelactone hydrolase family protein [Mycobacterium parascrofulaceum 
ATCC BAA-614]
Length=239

 Score =  377 bits (968),  Expect = 8e-103, Method: Compositional matrix adjust.
 Identities = 177/233 (76%), Positives = 200/233 (86%), Gaps = 0/233 (0%)

Query  1    MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN  60
            MTTIEID P GPIDALL LP GQGPWPGVVV+HDA GY  D +  ++RIA+AGY+ +TPN
Sbjct  7    MTTIEIDTPDGPIDALLDLPTGQGPWPGVVVIHDAFGYAHDKESTNKRIAQAGYIAITPN  66

Query  61   MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALV  120
            MYARGGR RCITRV +EL T+RGRALDDILAARDHL AMPECSG+VGI GFCMGGQFALV
Sbjct  67   MYARGGRLRCITRVMKELQTQRGRALDDILAARDHLKAMPECSGQVGIAGFCMGGQFALV  126

Query  121  LSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPA  180
            +SP+GFGA+APFYG PLPRHL +TL+GACPIVASFG RDPLG GA  +LR+  A KNI A
Sbjct  127  MSPKGFGASAPFYGAPLPRHLDKTLDGACPIVASFGARDPLGRGAPEKLRETIAKKNITA  186

Query  181  DIKSYPGAGHSFANKLPGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLRAGS  233
            D+K YPG GHSFAN+LP QPL+RI GFGY+ AATEDAWRRVF FFG+HL AG+
Sbjct  187  DVKVYPGVGHSFANELPAQPLLRITGFGYDRAATEDAWRRVFAFFGEHLAAGT  239


>gi|333990745|ref|YP_004523359.1| hypothetical protein JDM601_2105 [Mycobacterium sp. JDM601]
 gi|333486713|gb|AEF36105.1| conserved hypothetical protein [Mycobacterium sp. JDM601]
Length=233

 Score =  377 bits (967),  Expect = 1e-102, Method: Compositional matrix adjust.
 Identities = 179/231 (78%), Positives = 203/231 (88%), Gaps = 0/231 (0%)

Query  1    MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN  60
            MTTI++  P GPI+ALL +P G GPWPGVVVVHDA+GY  D K ISERIA AGY+ ++PN
Sbjct  1    MTTIDVATPDGPIEALLDIPEGPGPWPGVVVVHDAIGYAADMKAISERIADAGYLAVSPN  60

Query  61   MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALV  120
            +Y+RGGRARCITRVFRELLT+RGRALDDILAARDHLL MPECSG+VGI GFCMGGQF+LV
Sbjct  61   LYSRGGRARCITRVFRELLTQRGRALDDILAARDHLLGMPECSGKVGIAGFCMGGQFSLV  120

Query  121  LSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPA  180
            LSP+GFGA+APFYGTPLPRHL ETL+GACPIVASFG RDPLG GA +RLR+V    +IP 
Sbjct  121  LSPKGFGASAPFYGTPLPRHLEETLSGACPIVASFGGRDPLGRGAPDRLRRVVEHHDIPH  180

Query  181  DIKSYPGAGHSFANKLPGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLRA  231
            D+K+YPGAGHSFAN LP QPL+RIAGFGYNEAAT DAW RVF FFG+HL+A
Sbjct  181  DVKTYPGAGHSFANNLPAQPLLRIAGFGYNEAATADAWARVFAFFGEHLQA  231


>gi|342859769|ref|ZP_08716422.1| hypothetical protein MCOL_12858 [Mycobacterium colombiense CECT 
3035]
 gi|342132901|gb|EGT86121.1| hypothetical protein MCOL_12858 [Mycobacterium colombiense CECT 
3035]
Length=235

 Score =  375 bits (963),  Expect = 3e-102, Method: Compositional matrix adjust.
 Identities = 175/233 (76%), Positives = 203/233 (88%), Gaps = 0/233 (0%)

Query  1    MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN  60
            MTTI+ID P GPIDALL  P GQGPWPGVVV+HDA+GY  D + I++RIA AGY+ LTPN
Sbjct  1    MTTIQIDTPDGPIDALLSTPSGQGPWPGVVVIHDAIGYGRDKQSINDRIAAAGYLALTPN  60

Query  61   MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALV  120
            +Y+RGGR RCI+RV +EL T+RGRALDDILAARDHL AMP+CSG+VGI GFCMGGQFALV
Sbjct  61   LYSRGGRIRCISRVMKELQTQRGRALDDILAARDHLTAMPQCSGQVGIAGFCMGGQFALV  120

Query  121  LSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPA  180
            +SP+GFGA+APFYGTPLPR+L +TL+GACP+VASFG RDPLG GA  +L K  AAKNI A
Sbjct  121  MSPKGFGASAPFYGTPLPRNLDKTLDGACPVVASFGGRDPLGRGAPEKLSKTIAAKNITA  180

Query  181  DIKSYPGAGHSFANKLPGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLRAGS  233
            D+K+YPG GHSFAN LPGQPL+RIAGFGY+  ATEDAWRRVF FFG+HLRAG+
Sbjct  181  DVKTYPGVGHSFANDLPGQPLLRIAGFGYDHEATEDAWRRVFAFFGEHLRAGA  233


>gi|15827757|ref|NP_302020.1| dienelactone hydrolase [Mycobacterium leprae TN]
 gi|221230234|ref|YP_002503650.1| putative dienelactone hydrolase [Mycobacterium leprae Br4923]
 gi|13093309|emb|CAC30394.1| possible dienelactone hydrolase [Mycobacterium leprae]
 gi|219933341|emb|CAR71538.1| possible dienelactone hydrolase [Mycobacterium leprae Br4923]
Length=232

 Score =  370 bits (950),  Expect = 9e-101, Method: Compositional matrix adjust.
 Identities = 175/231 (76%), Positives = 197/231 (86%), Gaps = 0/231 (0%)

Query  1    MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN  60
            MT I+IDAP GPIDALL +PPG  PWPGVVV+HDA+GY PD +  +  IA AGYV +TPN
Sbjct  1    MTKIQIDAPDGPIDALLSVPPGPEPWPGVVVIHDAIGYEPDKESTNNHIAMAGYVAITPN  60

Query  61   MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALV  120
            +Y+RG RARCITRV RELLTKRGRA DDILA RD+LLAMP+CSGRVGI GFCMGG+FALV
Sbjct  61   LYSRGSRARCITRVMRELLTKRGRAFDDILATRDYLLAMPKCSGRVGIAGFCMGGRFALV  120

Query  121  LSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPA  180
            +SP+GF A+APFYGTPLPR+LSETLNGACPIVASFG RDPLGIGA  RLR+ T  ++I  
Sbjct  121  MSPKGFDASAPFYGTPLPRNLSETLNGACPIVASFGGRDPLGIGAPKRLRQATQTRHITT  180

Query  181  DIKSYPGAGHSFANKLPGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLRA  231
            DIK YP AGHSFANKLPGQPL+RI GFGYN+AATEDAW RVF FF +HLR 
Sbjct  181  DIKVYPDAGHSFANKLPGQPLMRITGFGYNQAATEDAWSRVFAFFDKHLRT  231


>gi|254775104|ref|ZP_05216620.1| hypothetical protein MaviaA2_10581 [Mycobacterium avium subsp. 
avium ATCC 25291]
Length=233

 Score =  367 bits (943),  Expect = 6e-100, Method: Compositional matrix adjust.
 Identities = 177/231 (77%), Positives = 196/231 (85%), Gaps = 0/231 (0%)

Query  1    MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN  60
            MTTI+ID P GPIDALL  P GQGPWPGVVV+HDA GY  D + I++RIARAGY+ LTPN
Sbjct  1    MTTIQIDTPDGPIDALLSTPAGQGPWPGVVVIHDAFGYGRDKQSINDRIARAGYLALTPN  60

Query  61   MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALV  120
            MYARGG  RCITRV +EL  +RGRALDDILAARDHL AMPEC+GRVGI GFCMGG+FALV
Sbjct  61   MYARGGLVRCITRVMKELAAQRGRALDDILAARDHLQAMPECTGRVGIAGFCMGGRFALV  120

Query  121  LSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPA  180
            +SP+GFGA+APFYGTPLP +L E L GACP+VASFG RD  G GAA +LRKVTA KNI A
Sbjct  121  MSPKGFGASAPFYGTPLPGNLDEVLEGACPVVASFGGRDLTGKGAAEKLRKVTADKNITA  180

Query  181  DIKSYPGAGHSFANKLPGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLRA  231
            DIK YP AGHSFAN+LP QPL+RIAGFGYNE ATEDAWRRVF FFG+HL A
Sbjct  181  DIKVYPEAGHSFANELPAQPLLRIAGFGYNEEATEDAWRRVFSFFGEHLAA  231


>gi|118472341|ref|YP_886784.1| dienelactone hydrolase [Mycobacterium smegmatis str. MC2 155]
 gi|118173628|gb|ABK74524.1| dienelactone hydrolase family protein [Mycobacterium smegmatis 
str. MC2 155]
Length=236

 Score =  365 bits (937),  Expect = 3e-99, Method: Compositional matrix adjust.
 Identities = 167/234 (72%), Positives = 199/234 (86%), Gaps = 0/234 (0%)

Query  3    TIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPNMY  62
            TI I  P GPIDALL  P G GPWPGVV++HDA+GY PDN+L+SER+A+AGY+ +TPN+Y
Sbjct  2    TITISTPDGPIDALLSTPTGSGPWPGVVIIHDAIGYGPDNELVSERVAQAGYLAITPNLY  61

Query  63   ARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALVLS  122
            +RGGRARCITR+FR+LLT+RGRALDDILAARDHL ++PEC+G VG+VGFC GGQFAL++ 
Sbjct  62   SRGGRARCITRMFRDLLTQRGRALDDILAARDHLKSLPECTGTVGVVGFCSGGQFALIMG  121

Query  123  PRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPADI  182
            P+GF A+APFYGTPLPRHL  TL+ +CPIVASFG RDPLGIGA  RLR+V  AKNIPADI
Sbjct  122  PKGFAASAPFYGTPLPRHLDRTLDASCPIVASFGRRDPLGIGAPARLRRVVEAKNIPADI  181

Query  183  KSYPGAGHSFANKLPGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLRAGSPGE  236
            K YP AGHSFAN LP QPL+R+ GFGY+EAAT DAW RVF FF +HL+  + G+
Sbjct  182  KVYPSAGHSFANILPAQPLLRVTGFGYDEAATTDAWTRVFAFFDEHLKPKTAGQ  235


>gi|41407901|ref|NP_960737.1| hypothetical protein MAP1803 [Mycobacterium avium subsp. paratuberculosis 
K-10]
 gi|41396255|gb|AAS04120.1| hypothetical protein MAP_1803 [Mycobacterium avium subsp. paratuberculosis 
K-10]
 gi|336462070|gb|EGO40915.1| dienelactone hydrolase-like enzyme [Mycobacterium avium subsp. 
paratuberculosis S397]
Length=233

 Score =  362 bits (930),  Expect = 2e-98, Method: Compositional matrix adjust.
 Identities = 175/231 (76%), Positives = 194/231 (84%), Gaps = 0/231 (0%)

Query  1    MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN  60
            MTTI+ID P GPIDALL  P GQGPWPGVVV+HDA GY  D + I++RIARAGY+ LTPN
Sbjct  1    MTTIQIDTPDGPIDALLSTPAGQGPWPGVVVIHDAFGYGRDKQSINDRIARAGYLALTPN  60

Query  61   MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALV  120
            MYARGG  RCITRV +EL  +RGRALDDILAARDHL AMPEC+GRVGI GFCMGG+FALV
Sbjct  61   MYARGGLVRCITRVMKELAAQRGRALDDILAARDHLQAMPECTGRVGIAGFCMGGRFALV  120

Query  121  LSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPA  180
            +SP+GF A+APFYGTPLP +L E L GACP+VASFG RD  G GA  +LRKVTA KNI A
Sbjct  121  MSPKGFSASAPFYGTPLPGNLDEVLEGACPVVASFGGRDLTGKGAPEKLRKVTADKNITA  180

Query  181  DIKSYPGAGHSFANKLPGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLRA  231
            DIK YP AGHSFAN+LP QPL+RIAGFGYNE ATEDAWRRVF FFG+HL A
Sbjct  181  DIKVYPVAGHSFANELPAQPLLRIAGFGYNEEATEDAWRRVFSFFGEHLAA  231


>gi|254819614|ref|ZP_05224615.1| hypothetical protein MintA_06804 [Mycobacterium intracellulare 
ATCC 13950]
Length=235

 Score =  362 bits (929),  Expect = 3e-98, Method: Compositional matrix adjust.
 Identities = 173/233 (75%), Positives = 195/233 (84%), Gaps = 0/233 (0%)

Query  1    MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN  60
            MTTI+ID P GPIDALL  P GQGPWPGVVV+HDA GY  D +  ++RIARAGY+ LTPN
Sbjct  1    MTTIQIDTPDGPIDALLSTPSGQGPWPGVVVIHDAFGYGRDKQSANDRIARAGYLALTPN  60

Query  61   MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALV  120
            MYARGGR RCI+RV +EL T+RGRA  DILAARDHL A+PECSG+VGI GFCMGGQFALV
Sbjct  61   MYARGGRIRCISRVMKELQTQRGRAFTDILAARDHLKAIPECSGQVGIAGFCMGGQFALV  120

Query  121  LSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPA  180
            +SP+GFGA APFY TPLPR+L +TL+GACPIVAS G RDPLG GA  +LRK  A KNI A
Sbjct  121  MSPKGFGAAAPFYSTPLPRNLDKTLDGACPIVASLGGRDPLGRGAPEKLRKTIADKNITA  180

Query  181  DIKSYPGAGHSFANKLPGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLRAGS  233
            D+K YPGAGHSFAN+LP Q L+RI GFGYNE ATEDAWRRVF FFG+HL AG+
Sbjct  181  DVKVYPGAGHSFANELPAQGLLRITGFGYNEDATEDAWRRVFAFFGEHLAAGA  233


>gi|126435120|ref|YP_001070811.1| dienelactone hydrolase [Mycobacterium sp. JLS]
 gi|126234920|gb|ABN98320.1| dienelactone hydrolase [Mycobacterium sp. JLS]
Length=228

 Score =  320 bits (821),  Expect = 8e-86, Method: Compositional matrix adjust.
 Identities = 153/227 (68%), Positives = 185/227 (82%), Gaps = 0/227 (0%)

Query  4    IEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPNMYA  63
            + +D P GPIDA L +P G GPWPGVVVVHDA+GY PD +  + RIA AG+V +TPN+Y+
Sbjct  1    MRVDTPDGPIDAALSVPEGDGPWPGVVVVHDAIGYGPDVEATAARIAAAGFVNITPNLYS  60

Query  64   RGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALVLSP  123
            RGGRARCITRVFRE L +RGRA DD+LAARDHLLA+P+CSG VGI GFCMGGQFAL+L P
Sbjct  61   RGGRARCITRVFREALQQRGRAFDDVLAARDHLLALPQCSGAVGIAGFCMGGQFALLLGP  120

Query  124  RGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPADIK  183
              FGA+APFYGTPLP+H+ ETL+ +CP+VASFG RDPLG  A  +L+++  AK +P+D+K
Sbjct  121  GNFGASAPFYGTPLPKHIDETLDASCPVVASFGRRDPLGKDAGPKLQEMLRAKGVPSDVK  180

Query  184  SYPGAGHSFANKLPGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLR  230
             YP AGHSFAN+LP QPL+RIA FGY+  AT+DAWRRVF FF  HL 
Sbjct  181  VYPDAGHSFANQLPAQPLMRIAQFGYHADATDDAWRRVFAFFDTHLN  227


>gi|108799466|ref|YP_639663.1| dienelactone hydrolase [Mycobacterium sp. MCS]
 gi|119868579|ref|YP_938531.1| dienelactone hydrolase [Mycobacterium sp. KMS]
 gi|108769885|gb|ABG08607.1| dienelactone hydrolase [Mycobacterium sp. MCS]
 gi|119694668|gb|ABL91741.1| dienelactone hydrolase [Mycobacterium sp. KMS]
Length=228

 Score =  320 bits (820),  Expect = 1e-85, Method: Compositional matrix adjust.
 Identities = 152/227 (67%), Positives = 185/227 (82%), Gaps = 0/227 (0%)

Query  4    IEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPNMYA  63
            + +D P GPIDA L +P G GPWPGVVVVHDA+GY PD +  + RIA AG+V +TPN+Y+
Sbjct  1    MRVDTPDGPIDAALSVPEGDGPWPGVVVVHDAIGYGPDVEATAARIAAAGFVNITPNLYS  60

Query  64   RGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALVLSP  123
            RGGRARCITRVFRE L +RGRA DD+LAARDHLLA+P+CSG +GI GFCMGGQFAL+L P
Sbjct  61   RGGRARCITRVFREALQQRGRAFDDVLAARDHLLALPQCSGAIGIAGFCMGGQFALLLGP  120

Query  124  RGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPADIK  183
              FGA+APFYGTPLP+H+ ETL+ +CP+VASFG RDPLG  A  +L+++  AK +P+D+K
Sbjct  121  GNFGASAPFYGTPLPKHIDETLDASCPVVASFGRRDPLGKDAGPKLQEMLRAKGVPSDVK  180

Query  184  SYPGAGHSFANKLPGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLR  230
             YP AGHSFAN+LP QPL+RIA FGY+  AT+DAWRRVF FF  HL 
Sbjct  181  VYPDAGHSFANQLPAQPLMRIAQFGYHADATDDAWRRVFAFFDTHLN  227


>gi|118467084|ref|YP_881637.1| dienelactone hydrolase [Mycobacterium avium 104]
 gi|118168371|gb|ABK69268.1| dienelactone hydrolase family protein [Mycobacterium avium 104]
Length=203

 Score =  315 bits (806),  Expect = 5e-84, Method: Compositional matrix adjust.
 Identities = 151/201 (76%), Positives = 170/201 (85%), Gaps = 0/201 (0%)

Query  31   VVHDAVGYVPDNKLISERIARAGYVVLTPNMYARGGRARCITRVFRELLTKRGRALDDIL  90
            ++HDA GY  D + I++RIARAGY+ LTPNMYARGG  RCITRV +EL  +RGRALDDIL
Sbjct  1    MIHDAFGYGRDKQSINDRIARAGYLALTPNMYARGGLVRCITRVMKELAAQRGRALDDIL  60

Query  91   AARDHLLAMPECSGRVGIVGFCMGGQFALVLSPRGFGATAPFYGTPLPRHLSETLNGACP  150
            AARDHL AMPEC+GRVGI GFCMGG+FALV+SP+GFGA+APFYGTPLP +L E L GACP
Sbjct  61   AARDHLQAMPECTGRVGIAGFCMGGRFALVMSPKGFGASAPFYGTPLPGNLDEVLEGACP  120

Query  151  IVASFGTRDPLGIGAANRLRKVTAAKNIPADIKSYPGAGHSFANKLPGQPLVRIAGFGYN  210
            +VASFG RD  G GA  +LRKVTA KNI ADIK YP AGHSFAN+LP QPL+RIAGFGYN
Sbjct  121  VVASFGGRDLTGKGAPEKLRKVTADKNITADIKVYPEAGHSFANELPAQPLLRIAGFGYN  180

Query  211  EAATEDAWRRVFEFFGQHLRA  231
            E ATEDAWRRVF FFG+HL A
Sbjct  181  EEATEDAWRRVFSFFGEHLAA  201


>gi|333918339|ref|YP_004491920.1| hypothetical protein AS9A_0666 [Amycolicicoccus subflavus DQS3-9A1]
 gi|333480560|gb|AEF39120.1| hypothetical protein AS9A_0666 [Amycolicicoccus subflavus DQS3-9A1]
Length=236

 Score =  276 bits (706),  Expect = 2e-72, Method: Compositional matrix adjust.
 Identities = 132/236 (56%), Positives = 171/236 (73%), Gaps = 7/236 (2%)

Query  1    MTTIEIDAPAGPIDALLGLPP---GQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVL  57
            MT I+  APAGP+   L LP    G  P+PGVVVVHDA+G   D     +RIA  GYVVL
Sbjct  1    MTDIQFAAPAGPMQGHLFLPSQADGATPYPGVVVVHDAIGMTDDTLESCQRIADKGYVVL  60

Query  58   TPNMYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQF  117
             P++Y+RGG+ARC+  V ++LL  RGR +DD+LAAR+HL+   +C+G VGI+GFCMGG F
Sbjct  61   APDLYSRGGKARCVPLVMKDLLLHRGRTVDDLLAAREHLVERADCTGAVGIIGFCMGGGF  120

Query  118  ALVLSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKN  177
            A++++P+GF A+APFYG  LPRHL+E L G+CP+VASFG RDPL IG   +L +      
Sbjct  121  AILMAPKGFDASAPFYGV-LPRHLTEALEGSCPMVASFGGRDPLLIGGEKKLVRALEKNG  179

Query  178  IPADIKSYPGAGHSFANKL---PGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLR  230
            +  D+K+YP  GHSFANK+   P  PL+R+ GF YN+AATEDAW RVF FFG+HL+
Sbjct  180  VEHDVKTYPKVGHSFANKIDLGPATPLLRVTGFAYNDAATEDAWHRVFAFFGEHLK  235


>gi|333919407|ref|YP_004492988.1| hypothetical protein AS9A_1739 [Amycolicicoccus subflavus DQS3-9A1]
 gi|333481628|gb|AEF40188.1| hypothetical protein AS9A_1739 [Amycolicicoccus subflavus DQS3-9A1]
Length=231

 Score =  269 bits (688),  Expect = 2e-70, Method: Compositional matrix adjust.
 Identities = 132/229 (58%), Positives = 162/229 (71%), Gaps = 1/229 (0%)

Query  1    MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN  60
            M  I IDAPAG ID     P G+GPWPGVVVVHD +G   D + I+ R A AG++ + P+
Sbjct  1    MDDIRIDAPAGTIDGAFEKPDGEGPWPGVVVVHDLLGLSTDARNITRRFADAGFLAVAPD  60

Query  61   MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALV  120
            +YARGG+ RC+ RV RE+L +RG A+DDILAARD L A P+CSG+VG+VGFC+GG FAL+
Sbjct  61   LYARGGKVRCVQRVMREMLMRRGNAVDDILAARDALTARPDCSGKVGVVGFCLGGGFALL  120

Query  121  LSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPA  180
            +SP+GFGA APFY + +P      + G C IVAS+G RDPL  G A RLR V   K I  
Sbjct  121  MSPKGFGAAAPFYPS-IPPLYDSVVTGGCAIVASYGKRDPLNPGNAGRLRTVLERKGIEH  179

Query  181  DIKSYPGAGHSFANKLPGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHL  229
            DIK Y  AGHSFAN +P QP+ RI GFGY+  ATEDA+ R F FF +HL
Sbjct  180  DIKVYANAGHSFANDVPVQPVARIVGFGYDAEATEDAFARTFAFFHKHL  228


>gi|312138239|ref|YP_004005575.1| carboxymethylenebutenolidase [Rhodococcus equi 103S]
 gi|311887578|emb|CBH46890.1| putative carboxymethylenebutenolidase [Rhodococcus equi 103S]
Length=238

 Score =  269 bits (687),  Expect = 3e-70, Method: Compositional matrix adjust.
 Identities = 130/240 (55%), Positives = 171/240 (72%), Gaps = 7/240 (2%)

Query  1    MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN  60
            M+ + +    GPIDA+L  P G GPWPGVVVVHDA+G   D + I+ R+A  GY+ LTP+
Sbjct  1    MSMVSVPTGQGPIDAVLETPDGTGPWPGVVVVHDALGIGRDIRDITARVAAHGYLTLTPD  60

Query  61   MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALV  120
            +Y RGGR  C+   FR+L+ +RGRA+DD+L+ARD L+A P+C+GRVG++GFCMGG FALV
Sbjct  61   LYTRGGRTWCVVGTFRDLIRRRGRAVDDLLSARDLLVARPDCTGRVGVIGFCMGGGFALV  120

Query  121  LSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPA  180
            L+P GF A APFYG PLPR + E L G+CPIV S+G RDP+  GA  +L        +  
Sbjct  121  LAPEGFDAAAPFYG-PLPRDIEEELGGSCPIVGSYGARDPMLRGAGRKLDSALTRLGVEH  179

Query  181  DIKSYPGAGHSFANKL-----PGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLRAGSPG  235
            D+++YPG GHSFAN+L      GQ L+R+ G GY++A ++DAWRRVF FFG HL  G+ G
Sbjct  180  DVQTYPGVGHSFANRLATGRYTGQ-LLRVTGMGYDDATSQDAWRRVFGFFGSHLSGGAEG  238


>gi|325674853|ref|ZP_08154540.1| dienelactone hydrolase [Rhodococcus equi ATCC 33707]
 gi|325554439|gb|EGD24114.1| dienelactone hydrolase [Rhodococcus equi ATCC 33707]
Length=238

 Score =  268 bits (685),  Expect = 5e-70, Method: Compositional matrix adjust.
 Identities = 129/240 (54%), Positives = 170/240 (71%), Gaps = 7/240 (2%)

Query  1    MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN  60
            M+ + +    GPIDA+L  P G GPWPGVVVVHDA+G   D + I+ R+A  GY+ LTP+
Sbjct  1    MSMVSVPTGQGPIDAVLETPDGTGPWPGVVVVHDALGIGRDIRDITARVAAHGYLTLTPD  60

Query  61   MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALV  120
            +Y RGGR RC+   FR+L+ +RGRA+DD+L+ARD L+A P+C+GRVG++GFCMGG FALV
Sbjct  61   LYTRGGRTRCVVGTFRDLIRRRGRAVDDLLSARDLLVARPDCTGRVGVIGFCMGGGFALV  120

Query  121  LSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPA  180
            L+P  F A APFYG PLPR + E L G+CPIV S+G RDP+  GA  +L        +  
Sbjct  121  LAPERFDAAAPFYG-PLPRDIEEALGGSCPIVGSYGARDPMLRGAGRKLDSALTRLGVEH  179

Query  181  DIKSYPGAGHSFANKL-----PGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLRAGSPG  235
            D+++YPG GHSFAN+L      GQ L+R+ G GY++  ++DAWRRVF FFG HL  G+ G
Sbjct  180  DVQTYPGVGHSFANRLATGRYTGQ-LLRVTGMGYDDVTSQDAWRRVFGFFGSHLSGGAEG  238


>gi|134101850|ref|YP_001107511.1| putative carboxymethylenebutenolidase precursor (dienelactone 
hydrolase) (DLH) [Saccharopolyspora erythraea NRRL 2338]
 gi|291009865|ref|ZP_06567838.1| putative carboxymethylenebutenolidase precursor (dienelactone 
hydrolase) (DLH) [Saccharopolyspora erythraea NRRL 2338]
 gi|133914473|emb|CAM04586.1| putative carboxymethylenebutenolidase precursor (dienelactone 
hydrolase) (DLH) [Saccharopolyspora erythraea NRRL 2338]
Length=236

 Score =  245 bits (626),  Expect = 3e-63, Method: Compositional matrix adjust.
 Identities = 127/238 (54%), Positives = 160/238 (68%), Gaps = 9/238 (3%)

Query  1    MTTIEIDAPAGPIDALLGLP----PGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVV  56
            M T +I  P G I A +  P     G GPWPGVVVVHDA G   D + I+ER A AGY+ 
Sbjct  1    MATAQISTPNGMIPAYVATPLPAVSGPGPWPGVVVVHDAAGLSDDIRAIAERFATAGYLA  60

Query  57   LTPNMYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQ  116
            + P++YARGG ARC+  V R+L   RG+A DD+ AAR HL    +C+G+VG+ GFCMGG 
Sbjct  61   VAPDLYARGGFARCVRSVMRDLQQARGQAFDDVDAARSHLTGRDDCTGKVGVAGFCMGGG  120

Query  117  FALVLSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAK  176
            FA++ + RGF A+AP+YG  LP   S  L+GACPIVASFG +DP+  GAA +L    +A+
Sbjct  121  FAIIGASRGFDASAPYYGQ-LPSDPS-VLDGACPIVASFGAKDPMLRGAAAKLESELSAR  178

Query  177  NIPADIKSYPGAGHSFANKLPGQP---LVRIAGFGYNEAATEDAWRRVFEFFGQHLRA  231
             I  D+K YP AGHSFAN+LP  P   L R+AGFGY+   +EDAWRRV  FF +HL A
Sbjct  179  GITHDVKEYPDAGHSFANRLPLGPFNVLARVAGFGYHHETSEDAWRRVQNFFAEHLTA  236


>gi|300790960|ref|YP_003771251.1| carboxymethylenebutenolidase [Amycolatopsis mediterranei U32]
 gi|299800474|gb|ADJ50849.1| carboxymethylenebutenolidase [Amycolatopsis mediterranei U32]
 gi|340532656|gb|AEK47861.1| carboxymethylenebutenolidase [Amycolatopsis mediterranei S699]
Length=238

 Score =  233 bits (595),  Expect = 1e-59, Method: Compositional matrix adjust.
 Identities = 118/224 (53%), Positives = 151/224 (68%), Gaps = 6/224 (2%)

Query  11   GPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPNMYARGGRARC  70
            G I   L  P G GP PGVV++HDA G   D K I++R AR GY+ L+P++Y+RGG  RC
Sbjct  10   GTIGGYLAEPAGAGPHPGVVLIHDAFGLSQDTKNITDRFAREGYLALSPDLYSRGGMFRC  69

Query  71   ITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALVLSPRGFGATA  130
            + RVF ++   RGRA DDI A+R  L A P+CSGR+GI GFCMGG FALV + RGF A+A
Sbjct  70   VRRVFSDMFANRGRAFDDIEASRALLAARPDCSGRIGIAGFCMGGGFALVAASRGFEASA  129

Query  131  PFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPADIKSYPGAGH  190
            P+YG  LP++LS  L+ ACP+VASFG +D    GAA +L  V   + IP D+K YP A H
Sbjct  130  PYYGQ-LPKNLS-VLDDACPVVASFGKKDFGLRGAAAKLETVLTERGIPHDVKEYPDASH  187

Query  191  SFANKL----PGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLR  230
             FANK+    P   L+++ GF Y+  A EDAW RV  FFG+HL+
Sbjct  188  GFANKIDTAAPVNLLLKVVGFTYHHEAAEDAWSRVTSFFGEHLQ  231


>gi|326332773|ref|ZP_08199034.1| putative carboxymethylenebutenolidase [Nocardioidaceae bacterium 
Broad-1]
 gi|325949472|gb|EGD41551.1| putative carboxymethylenebutenolidase [Nocardioidaceae bacterium 
Broad-1]
Length=233

 Score =  230 bits (586),  Expect = 1e-58, Method: Compositional matrix adjust.
 Identities = 118/231 (52%), Positives = 153/231 (67%), Gaps = 7/231 (3%)

Query  4    IEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPNMYA  63
            I+  APAGP    L +P G GPWPGVVVV D +G   D + I +RIA  GY+ LTP +Y 
Sbjct  2    IDYAAPAGPTRGYLAVPEGDGPWPGVVVVQDLMGVSADVQRIGDRIAANGYLALTPELYH  61

Query  64   RGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALVLSP  123
            RG +  C+    + L+T +G A+DD++AARDHL+A P C+G+VGIVGFCMGG F L+L+P
Sbjct  62   RGPKVGCVVSTMKSLVTGKGTAVDDLIAARDHLVADPRCTGKVGIVGFCMGGGFTLLLAP  121

Query  124  RG-FGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPADI  182
            RG F A AP YG  LPR LS  L+ +CP+VASFG +D L  GA +++    AA ++P D+
Sbjct  122  RGIFDAAAPNYGV-LPRDLS-ALSSSCPMVASFGAKDRLLPGAVHKVEAALAAGDVPRDL  179

Query  183  KSYPGAGHSFANKL----PGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHL  229
            K YP  GHSF N      P   L R+ G+ Y+E   EDAWRR+F FF +HL
Sbjct  180  KEYPEVGHSFMNDWGFPRPVPTLERVVGYNYSEPEAEDAWRRIFAFFDEHL  230


>gi|54024910|ref|YP_119152.1| putative hydrolase [Nocardia farcinica IFM 10152]
 gi|54016418|dbj|BAD57788.1| putative hydrolase [Nocardia farcinica IFM 10152]
Length=235

 Score =  223 bits (567),  Expect = 2e-56, Method: Compositional matrix adjust.
 Identities = 117/237 (50%), Positives = 151/237 (64%), Gaps = 8/237 (3%)

Query  1    MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN  60
            M TI++ AP GPI+ALL  P   GPWPG+V++HDA+ +  D +     +A  GY+V+ PN
Sbjct  1    MQTIDLPAPDGPIEALLARPDTPGPWPGIVIIHDALEFGADIRTHVTMLAERGYLVIAPN  60

Query  61   MYARGGRARCITRVFRELLTKRGRA------LDDILAARDHLLAMPECSGRVGIVGFCMG  114
            +++RG +ARCI  V R L    GR       + DILAARDHLLA P+C+GR  + GFCMG
Sbjct  61   LFSRG-KARCIRAVMRALWFTGGRNHAEAVPVRDILAARDHLLADPDCTGRTAVAGFCMG  119

Query  115  GQFALVLSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTA  174
            G FAL+ +P GF A APFY   L       L GACP+VAS+   DP  IGAA +L +   
Sbjct  120  GGFALLTAPHGFDAAAPFY-PGLYGDYRTLLEGACPVVASYAGLDPSLIGAAGKLDRALT  178

Query  175  AKNIPADIKSYPGAGHSFANKLPGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLRA  231
               +P DIK+YP   H FAN    + L+R+ G G++EAAT DAWRR+F FF QHL A
Sbjct  179  DLGVPHDIKTYPRTTHGFANTFGTERLLRVVGLGHDEAATADAWRRIFAFFDQHLTA  235


>gi|296393433|ref|YP_003658317.1| dienelactone hydrolase [Segniliparus rotundus DSM 44985]
 gi|296180580|gb|ADG97486.1| dienelactone hydrolase [Segniliparus rotundus DSM 44985]
Length=248

 Score =  212 bits (539),  Expect = 4e-53, Method: Compositional matrix adjust.
 Identities = 118/244 (49%), Positives = 153/244 (63%), Gaps = 13/244 (5%)

Query  2    TTIEIDAPAGPIDALLGLP--PGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTP  59
            +TI++ APAGPI   L  P  P  GP PGVV++HD  G V D + I++++A  GY+V  P
Sbjct  6    STIDVQAPAGPISVELFTPHNPPHGPVPGVVMIHDISGPVADLRRIAQKVADQGYLVALP  65

Query  60   NMYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFAL  119
            ++YARGG+++C+    R +L+ RG A DDI A R  L A P C+GR  I GFC+GG FAL
Sbjct  66   DLYARGGKSKCLVGTVRAMLSGRGSAFDDIAALRAALAADPRCTGRTAITGFCLGGGFAL  125

Query  120  VLSPR-GFGATAPFYGT-----PLPRHLSETLNGACPIVASFGTRDP-LGIGAANRLRKV  172
            + +PR  F  +APFY +      LP+   E L  ACP+VAS G RDP LG  A   L + 
Sbjct  126  LFAPREQFDVSAPFYPSNMIRGKLPKGFEELLERACPVVASLGGRDPLLGKKAGTVLERA  185

Query  173  TAAKNIPADIKSYPGAGHSFANKLPGQP---LVRIAGFGYNEAATEDAWRRVFEFFGQHL  229
             A   +P D+K YP AGHSFAN+ P +P   L R+AGFGY   + EDAW RVF FF +HL
Sbjct  186  LARHGVPHDVKVYPDAGHSFANQFP-EPVGTLARVAGFGYEHQSAEDAWERVFRFFEEHL  244

Query  230  RAGS  233
               S
Sbjct  245  LPSS  248


>gi|317506550|ref|ZP_07964345.1| dienelactone hydrolase [Segniliparus rugosus ATCC BAA-974]
 gi|316255147|gb|EFV14422.1| dienelactone hydrolase [Segniliparus rugosus ATCC BAA-974]
Length=741

 Score =  210 bits (535),  Expect = 1e-52, Method: Compositional matrix adjust.
 Identities = 108/241 (45%), Positives = 148/241 (62%), Gaps = 11/241 (4%)

Query  2    TTIEIDAPAGPI--DALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTP  59
            +T+++ APAG I  +      P +G  PGVV++HD +G V D + +++++A  GY+V  P
Sbjct  499  STLDVPAPAGSIAVEVFTAQKPSEGSVPGVVMIHDILGVVDDVRRLAQKVADQGYIVALP  558

Query  60   NMYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFAL  119
            ++YARGG+ +C+    R +LT  G A DDI A R  L A P C+GR+ I GFC+GG +AL
Sbjct  559  DLYARGGKRKCLLSTARSMLTGEGTAFDDITAVRHALAADPRCTGRIAITGFCLGGGYAL  618

Query  120  VLSP-RGFGATAPFYGT-----PLPRHLSETLNGACPIVASFGTRDP-LGIGAANRLRKV  172
            + +P + F   APFY +      LPR   E L  +CP+VAS G+RDP LG  A   L   
Sbjct  619  LFAPSKQFDVAAPFYPSNVTRGKLPRKFEELLEHSCPVVASLGSRDPMLGKKAGPVLEHA  678

Query  173  TAAKNIPADIKSYPGAGHSFANKLPGQ--PLVRIAGFGYNEAATEDAWRRVFEFFGQHLR  230
             +   IP D+K Y GAGHSFAN+ PG    L ++ GF Y   A EDAW+RVF FF +HL 
Sbjct  679  LSTHGIPNDVKVYQGAGHSFANQFPGTIGTLAKVVGFSYAHEAAEDAWQRVFSFFEEHLV  738

Query  231  A  231
            A
Sbjct  739  A  739


>gi|148272701|ref|YP_001222262.1| putative carboxymethylenebutenolidase [Clavibacter michiganensis 
subsp. michiganensis NCPPB 382]
 gi|147830631|emb|CAN01567.1| putative carboxymethylenebutenolidase [Clavibacter michiganensis 
subsp. michiganensis NCPPB 382]
Length=247

 Score =  199 bits (505),  Expect = 3e-49, Method: Compositional matrix adjust.
 Identities = 108/241 (45%), Positives = 143/241 (60%), Gaps = 12/241 (4%)

Query  1    MTTIEIDAPAGPIDA------LLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGY  54
            MT  EI  PA   DA      +L +P G+GPWP VVVVH+A G     +  +ER+A AG+
Sbjct  1    MTLTEIPLPAASPDASPGLHGVLAVPEGEGPWPAVVVVHEAFGVTDVMRRQAERLAEAGF  60

Query  55   VVLTPNMYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMG  114
            + L P++++ GG  RC+   FR L    GRA  D+ +AR  LLA  +C+GRVG++GFCMG
Sbjct  61   LALMPDLFSAGGARRCLVATFRTLAAGEGRAFVDVESARRALLARADCTGRVGVIGFCMG  120

Query  115  GQFALVLSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTA  174
            G FAL  + RGF A++  YG  LP  L   L+GACPIVAS+G RD    G+A+RL     
Sbjct  121  GGFALAAASRGFDASSVNYGM-LPEDLDGVLDGACPIVASYGGRDRQLAGSADRLEAALV  179

Query  175  AKNIPADIKSYPGAGHSFANKLPG-----QPLVRIAGFGYNEAATEDAWRRVFEFFGQHL  229
            A+ I  D++ Y  AGHSF N         +P++R  G G   A+  DAWRR+  F   HL
Sbjct  180  ARGIDHDVREYDAAGHSFLNDAEMGPRALRPVLRAVGMGPEPASAADAWRRIDAFLDAHL  239

Query  230  R  230
            R
Sbjct  240  R  240


>gi|312199662|ref|YP_004019723.1| carboxymethylenebutenolidase [Frankia sp. EuI1c]
 gi|311230998|gb|ADP83853.1| carboxymethylenebutenolidase [Frankia sp. EuI1c]
Length=234

 Score =  191 bits (484),  Expect = 1e-46, Method: Compositional matrix adjust.
 Identities = 99/214 (47%), Positives = 135/214 (64%), Gaps = 5/214 (2%)

Query  20   PPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPNMYARGGRARCITRVFRELL  79
            P G GPWPG+V+VHDA G   + +  ++R+A AGYV + PN+Y+ GG  RC+    R  +
Sbjct  23   PAGSGPWPGIVIVHDAFGLTDNARKHADRLAAAGYVTVVPNLYSAGGL-RCVRATIRAAV  81

Query  80   TKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALVLSPRGFGATAPFYGTPLPR  139
               G A  D+ A R +L A  +C+ R+G+VGFCMGG FAL+ + RGF A+AP YG     
Sbjct  82   AGTGAAHADLEAVRAYLAARDDCTRRIGVVGFCMGGGFALMAATRGFDASAPNYGMLPKG  141

Query  140  HLSETLNGACPIVASFGTRDPLGI-GAANRLRKVTAAKNIPADIKSYPGAGHSFANKLP-  197
             L E L GACP+VAS+G RD LG+ GAA ++     A   P D+K YP AGHSF ++ P 
Sbjct  142  DLEELLAGACPVVASYGARD-LGLRGAAAKIETALTAVGAPHDVKEYPKAGHSFMDRYPV  200

Query  198  -GQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLR  230
                + ++ G  ++  A EDAWRR+  FFG+HLR
Sbjct  201  LSGAVGKVIGMHHDGPAAEDAWRRILAFFGEHLR  234


>gi|170782159|ref|YP_001710492.1| putative hydrolase [Clavibacter michiganensis subsp. sepedonicus]
 gi|169156728|emb|CAQ01888.1| putative hydrolase [Clavibacter michiganensis subsp. sepedonicus]
Length=254

 Score =  190 bits (482),  Expect = 2e-46, Method: Compositional matrix adjust.
 Identities = 103/240 (43%), Positives = 142/240 (60%), Gaps = 12/240 (5%)

Query  1    MTTIEIDAPA-GP-----IDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGY  54
            +T  EI  PA GP     +  +L +P G+GPWP VVVVH+A G     +  +ER+A AG+
Sbjct  3    VTLTEIPLPAAGPGASPGLHGVLAVPDGEGPWPAVVVVHEAFGVTDVMRRQAERLAEAGF  62

Query  55   VVLTPNMYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMG  114
            + L P++++ GG  RC+   FR L    GRA  D+ +AR  LL   +C+GRVG++GFCMG
Sbjct  63   LALMPDLFSAGGARRCLVATFRTLAAGEGRAFVDVESARRLLLDRADCTGRVGVIGFCMG  122

Query  115  GQFALVLSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTA  174
            G FAL  + RGF A++  YG  LP  L   L+GACP+VAS+G RD    G+A+RL     
Sbjct  123  GGFALAAASRGFDASSVNYGM-LPEGLDGVLDGACPVVASYGGRDRQLAGSADRLEAALT  181

Query  175  AKNIPADIKSYPGAGHSFANKLPG-----QPLVRIAGFGYNEAATEDAWRRVFEFFGQHL  229
               I  D++ YP AGH+F N         +P++R  G G   A+  DAW R+  FF +HL
Sbjct  182  EHGIAHDVREYPAAGHAFLNDAETGPRALRPVLRAVGMGPEPASAADAWSRIDTFFSEHL  241


>gi|118465396|ref|YP_879769.1| dienelactone hydrolase [Mycobacterium avium 104]
 gi|118166683|gb|ABK67580.1| dienelactone hydrolase family protein [Mycobacterium avium 104]
Length=240

 Score =  189 bits (479),  Expect = 4e-46, Method: Compositional matrix adjust.
 Identities = 99/241 (42%), Positives = 138/241 (58%), Gaps = 12/241 (4%)

Query  1    MTTIEI------DAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGY  54
            MT I++       A + P+   L  P G GPWPGVV++H+  G     +  ++R+A  GY
Sbjct  1    MTDIDLTDLAAAHAGSQPLRGYLATPSGTGPWPGVVLIHEVFGIDDVMRRHTDRLAGLGY  60

Query  55   VVLTPNMYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMG  114
            + L  ++++ GG ARC+      ++  RGRA  DI AARD+L    +C+G+VG++GFCMG
Sbjct  61   LTLAVDLFSAGGAARCLISTMTAMIRGRGRAFADIAAARDYLAGSADCTGKVGVIGFCMG  120

Query  115  GQFALVLSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTA  174
            G FAL+ +  G+ A A  YG  LPRHL   L GACPIV S+G RDP   GA  +L +   
Sbjct  121  GGFALLTANTGYDAAAVNYGQ-LPRHLDTALAGACPIVGSYGGRDPSLRGATRKLNEALT  179

Query  175  AKNIPADIKSYPGAGHSFANKLPG-----QPLVRIAGFGYNEAATEDAWRRVFEFFGQHL  229
               I  D+K YP A H+F N         +PL+R+AG G    + +DAW R+  FF  HL
Sbjct  180  KAGIEHDVKEYPAASHAFLNDDEAGPRILRPLLRVAGIGPEPDSAQDAWNRIAAFFATHL  239

Query  230  R  230
             
Sbjct  240  H  240


>gi|111019264|ref|YP_702236.1| carboxymethylenebutenolidase [Rhodococcus jostii RHA1]
 gi|110818794|gb|ABG94078.1| probable carboxymethylenebutenolidase [Rhodococcus jostii RHA1]
Length=256

 Score =  186 bits (471),  Expect = 3e-45, Method: Compositional matrix adjust.
 Identities = 100/240 (42%), Positives = 144/240 (60%), Gaps = 12/240 (5%)

Query  1    MTTIEIDAPAGPIDALLGL------PPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGY  54
            M+ I++   A   D  L L      P G+GPWPGVV+VH+A G     +  ++R+A AG+
Sbjct  17   MSEIDLTDLAARHDGSLNLHGYLARPSGEGPWPGVVMVHEAFGLDEVMRRQADRLAAAGF  76

Query  55   VVLTPNMYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMG  114
            + L P++++ GG  RC+    R +L+ +GRA  DI AAR  L    +C+G++G++GFCMG
Sbjct  77   LTLAPDLFSAGGARRCLVSTMRAMLSGKGRAYGDIEAARQWLSGSSDCTGKIGVIGFCMG  136

Query  115  GQFALVLSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTA  174
            G FAL+    GF A AP YG  LPR L   + GACP+VAS+G +D     AA +L     
Sbjct  137  GGFALMTLRTGFDAAAPNYGV-LPRDLDAAVAGACPVVASYGGKDFALRNAAAKLESALT  195

Query  175  AKNIPADIKSYPGAGHSFAN-KLPG----QPLVRIAGFGYNEAATEDAWRRVFEFFGQHL  229
               +  D+K YP AGH+F N ++ G    +PLV++AG   +  A  DAW+R+  FFG+HL
Sbjct  196  KAGVAHDVKEYPTAGHAFLNDEVAGPKVLRPLVKVAGMVPDPEAAADAWKRIDGFFGEHL  255


>gi|297163413|gb|ADI13125.1| putative carboxymethylenebutenolidase [Streptomyces bingchenggensis 
BCW-1]
Length=250

 Score =  186 bits (471),  Expect = 4e-45, Method: Compositional matrix adjust.
 Identities = 105/235 (45%), Positives = 138/235 (59%), Gaps = 10/235 (4%)

Query  5    EIDAPAGPIDALLG---LPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPNM  61
             + APAG    L G    P G GPWPGVV VH+A+G        +ER++RAGY+ L P++
Sbjct  11   HLSAPAGGSRRLTGHLARPEGPGPWPGVVAVHEALGVDDVMLRQAERLSRAGYLALMPDL  70

Query  62   YARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALVL  121
            + +GG  RC+    R  L+  GRA  DI AAR  L    EC+G VGI+GFCMGG FAL+ 
Sbjct  71   FTQGGVRRCLVPTMRASLSGHGRAYQDIAAARTFLAESTECTGAVGIIGFCMGGAFALMS  130

Query  122  SPR-GFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPA  180
            +   GF   +  YG  LP+ L ETL+GACP+VAS+G RD +  GAA +L        +  
Sbjct  131  AGSGGFDVASANYGM-LPKELDETLSGACPVVASYGGRDRMLKGAAAKLDSALERLGVVH  189

Query  181  DIKSYPGAGHSFANKL-----PGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLR  230
            D+K YP AGH+F N       P +PL R+ G G +  A  DAW+R+  FF  HL+
Sbjct  190  DVKEYPQAGHAFLNDAEVGPRPLRPLFRVTGMGPHPEAAADAWQRIETFFDTHLK  244


>gi|226361394|ref|YP_002779172.1| carboxymethylenebutenolidase [Rhodococcus opacus B4]
 gi|226239879|dbj|BAH50227.1| putative carboxymethylenebutenolidase [Rhodococcus opacus B4]
Length=240

 Score =  183 bits (465),  Expect = 2e-44, Method: Compositional matrix adjust.
 Identities = 100/240 (42%), Positives = 143/240 (60%), Gaps = 12/240 (5%)

Query  1    MTTIEIDAPAGPIDALLGL------PPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGY  54
            M+ I++   A   D  L L      P G+GPWPGVV+VH+A G     +  ++R+A AG+
Sbjct  1    MSEIDLTDLAARHDGSLNLHGYLARPSGEGPWPGVVMVHEAFGLDDVMRRQADRLASAGF  60

Query  55   VVLTPNMYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMG  114
            + LTP++++ GG  RC+    R +L+ +GRA  DI AAR  L    +C+G++G++GFCMG
Sbjct  61   LTLTPDLFSAGGARRCLVSTMRAMLSGKGRAYGDIEAARQWLSGSSDCTGKIGVIGFCMG  120

Query  115  GQFALVLSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTA  174
            G FAL+    GF A AP YG  LPR L   + GACP+VAS+G +D     AA +L     
Sbjct  121  GGFALMTLRTGFDAAAPNYGV-LPRDLEAAVAGACPVVASYGGKDFALPNAAAKLESALT  179

Query  175  AKNIPADIKSYPGAGHSFAN-KLPG----QPLVRIAGFGYNEAATEDAWRRVFEFFGQHL  229
               +  D+K YP AGH+F N +L G    +PL+++ G   +  A  DAW R+  FFG+HL
Sbjct  180  RAGVVHDVKEYPTAGHTFLNDELAGPKVMRPLMKVTGMVPDPEAAADAWTRIDGFFGEHL  239


>gi|156742170|ref|YP_001432299.1| dienelactone hydrolase [Roseiflexus castenholzii DSM 13941]
 gi|156233498|gb|ABU58281.1| dienelactone hydrolase [Roseiflexus castenholzii DSM 13941]
Length=252

 Score =  182 bits (461),  Expect = 4e-44, Method: Compositional matrix adjust.
 Identities = 98/238 (42%), Positives = 138/238 (58%), Gaps = 9/238 (3%)

Query  1    MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN  60
            M  + I AP G + A L  P G GPWPGVVV+HDA+G   D    ++ +A  G++   P+
Sbjct  16   MADVVIHAPDGALHAYLATPDGTGPWPGVVVIHDALGMRSDTHRQADWLAGEGFLTAAPD  75

Query  61   MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALV  120
            ++  G    C+  + R+ LT +G     I A RD L    +C+GR+G++GFCMGG FAL+
Sbjct  76   LFDGGTVITCLRSIIRDYLTWQGMMFRHIAATRDWLAQRNDCTGRIGVIGFCMGGGFALL  135

Query  121  LSP-RGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIP  179
            L+P  GF A++  YG  LP  L   + GACPIVA++G +D +  GAA RL +   A  I 
Sbjct  136  LAPGHGFAASSVNYGI-LPDDLERAVTGACPIVANYGAKDAMLRGAAGRLERALTALGID  194

Query  180  ADIKSYPGAGHSFANKL---PGQPLVRIA----GFGYNEAATEDAWRRVFEFFGQHLR  230
             D+K YP AGH+F N         L+R+A      GY+E +  DA +R+  FF +HLR
Sbjct  195  HDVKEYPDAGHAFLNNHDPREVHALLRVAWHVTQMGYHEPSARDARQRIVTFFNRHLR  252


>gi|148656803|ref|YP_001277008.1| dienelactone hydrolase [Roseiflexus sp. RS-1]
 gi|148568913|gb|ABQ91058.1| dienelactone hydrolase [Roseiflexus sp. RS-1]
Length=237

 Score =  179 bits (453),  Expect = 4e-43, Method: Compositional matrix adjust.
 Identities = 98/237 (42%), Positives = 138/237 (59%), Gaps = 9/237 (3%)

Query  1    MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN  60
            M  I I AP G ++A L  P G GPWPGVVV+HDA+G   D +   E +A  G++ + P+
Sbjct  1    MADIVIPAPDGALNAYLATPDGNGPWPGVVVIHDALGMRRDTRQQVEWLASEGFLAIAPD  60

Query  61   MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALV  120
            ++  G    C+  + R+ LT +G     I AARD L    +C+G +G++GFCMGG FAL+
Sbjct  61   LFDGGTVITCLRLIIRDYLTWQGMMFRHIAAARDWLAQRDDCTGNIGVIGFCMGGGFALL  120

Query  121  LSP-RGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIP  179
            L+P  GF A++  YG  LP+ +   L GACPIVAS+G +D    GAA RL +      I 
Sbjct  121  LAPGHGFAASSVNYGV-LPKEIERVLAGACPIVASYGAKDITLRGAAQRLEQALTTLGIE  179

Query  180  ADIKSYPGAGHSFANKLPGQP---LVRIAGFG----YNEAATEDAWRRVFEFFGQHL  229
             D+K YP AGH+F N+   +    L+RI        Y+E +  DA +R+  FF +HL
Sbjct  180  HDVKEYPDAGHAFLNEHDPRDFPALMRIVWRATQTTYHEPSARDARQRIIAFFKRHL  236


>gi|226308781|ref|YP_002768741.1| carboxymethylenebutenolidase [Rhodococcus erythropolis PR4]
 gi|226187898|dbj|BAH36002.1| putative carboxymethylenebutenolidase [Rhodococcus erythropolis 
PR4]
Length=239

 Score =  177 bits (450),  Expect = 9e-43, Method: Compositional matrix adjust.
 Identities = 94/223 (43%), Positives = 132/223 (60%), Gaps = 7/223 (3%)

Query  12   PIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPNMYARGGRARCI  71
            P+ A+L  P   GPWPGVV++H+A G     +  ++R+A AG++ L  ++Y+ GG  RC+
Sbjct  18   PLRAILETPTTPGPWPGVVMIHEAFGLEEVMQRQAKRLASAGFLTLAVDLYSAGGAKRCL  77

Query  72   TRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALVLSPRGFGATAP  131
                  +L   G+A  DI  AR  L+  P+C+G++G++GFCMGG FA+V S   F A + 
Sbjct  78   VPTMTSMLRGHGKAFTDIEVARSWLVESPDCTGKIGVIGFCMGGGFAMV-SAGDFDAASV  136

Query  132  FYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPADIKSYPGAGHS  191
             YG  LPR L E +  ACPIV SFG +D    GAA +L    +   I  D+K YP AGH+
Sbjct  137  NYGQ-LPRKLDEAVVDACPIVGSFGGKDFSLRGAAAKLETALSNAGIENDVKEYPTAGHA  195

Query  192  FANKLPG-----QPLVRIAGFGYNEAATEDAWRRVFEFFGQHL  229
            F N         +PL RI G G +  A +DAW+R+  FFG+HL
Sbjct  196  FLNDAEAGPKVLRPLERILGIGPDPVAAQDAWKRIDSFFGRHL  238


>gi|111224276|ref|YP_715070.1| putative carboxymethylenebutenolidase [Frankia alni ACN14a]
 gi|111151808|emb|CAJ63528.1| Putative carboxymethylenebutenolidase precursor (Dienelactone 
hydrolase) (DLH) [Frankia alni ACN14a]
Length=262

 Score =  176 bits (447),  Expect = 2e-42, Method: Compositional matrix adjust.
 Identities = 95/216 (44%), Positives = 128/216 (60%), Gaps = 10/216 (4%)

Query  25   PWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPNMYARGGRARCITRVFRELLTKRGR  84
            P PGV+V+H+A G     + I++R+A  GY+ L P++++ GG  RC+    R + + RGR
Sbjct  48   PRPGVIVLHEAWGLDETIRAIADRLAGLGYLALAPDLFSAGGARRCLVATVRAMSSGRGR  107

Query  85   ALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALVLSPRGFGATAPFYGTPLPRHLSET  144
            A DDI AAR  L A+P C+GRVG++GFC+GG  AL+ + RGF   A  YG PLPR L  +
Sbjct  108  AFDDIEAARRWLTALPACNGRVGVIGFCLGGGLALLSAGRGFEVAAVNYG-PLPRELDAS  166

Query  145  LNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPADIKSYPGAGHSFANKLPG------  198
            L  ACP+VAS+G RD    GAA RL        +P D+K YP AGH+F +  P       
Sbjct  167  LRAACPVVASYGGRDRTLRGAAGRLSAALGRAGVPHDVKEYPTAGHAFLSARPDGTPSGP  226

Query  199  ---QPLVRIAGFGYNEAATEDAWRRVFEFFGQHLRA  231
                P++R AG G    A  DAW R+  F  +HL A
Sbjct  227  RVLHPVLRAAGIGPEPLAAADAWVRIERFLTEHLGA  262


>gi|229489128|ref|ZP_04382994.1| dienelactone hydrolase family protein [Rhodococcus erythropolis 
SK121]
 gi|229324632|gb|EEN90387.1| dienelactone hydrolase family protein [Rhodococcus erythropolis 
SK121]
Length=238

 Score =  171 bits (434),  Expect = 7e-41, Method: Compositional matrix adjust.
 Identities = 92/223 (42%), Positives = 129/223 (58%), Gaps = 7/223 (3%)

Query  12   PIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPNMYARGGRARCI  71
            P+ A+L  P   GPWPGVV++H+A G     +  ++R+A AG++ L  ++Y+ GG  RC+
Sbjct  17   PLRAILETPTTPGPWPGVVMIHEAFGLEEVMQRQAKRLAAAGFLTLAVDLYSAGGAKRCL  76

Query  72   TRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALVLSPRGFGATAP  131
                  +L   G+A  DI  AR  L+   +C+G++G++GFCMGG FA+V S   F A + 
Sbjct  77   VPTMTSMLRGHGKAFTDIEVARSWLVESTDCTGKIGVIGFCMGGGFAMV-SAGDFDAASV  135

Query  132  FYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPADIKSYPGAGHS  191
             YG  LPR L E +  ACPIV SFG +D    GAA +L        I  D+K YP AGH+
Sbjct  136  NYGQ-LPRKLDEAVVDACPIVGSFGGKDFSLRGAAAKLETALDNAGIENDVKEYPTAGHA  194

Query  192  FANKLPG-----QPLVRIAGFGYNEAATEDAWRRVFEFFGQHL  229
            F N         +PL RI G G +  A +DAW+R+  FF +HL
Sbjct  195  FLNDAEAGPKVLRPLERIVGIGPDPVAAQDAWKRIDSFFERHL  237


>gi|163841096|ref|YP_001625501.1| carboxymethylenebutenolidase [Renibacterium salmoninarum ATCC 
33209]
 gi|162954572|gb|ABY24087.1| carboxymethylenebutenolidase [Renibacterium salmoninarum ATCC 
33209]
Length=248

 Score =  164 bits (414),  Expect = 1e-38, Method: Compositional matrix adjust.
 Identities = 83/225 (37%), Positives = 124/225 (56%), Gaps = 7/225 (3%)

Query  13   IDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPNMYARGGRARCIT  72
            ++  L  P G+GPWPGVV++H+A G     +  ++R+A AGY+ L  ++++ GG  RC+ 
Sbjct  20   LNGYLATPAGEGPWPGVVMIHEATGLDDVQRRHADRLAGAGYLTLAADLFSDGGARRCLV  79

Query  73   RVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALVLSPRGFGATAPF  132
               R +   +GRA  DI A+R  LL    C+G+ G++GFCMGG FAL+ +  G    +  
Sbjct  80   STMRAMRAGQGRAFADIEASRQWLLKSELCNGQTGVIGFCMGGGFALLTAKMGHHVASVN  139

Query  133  YGT--PLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPADIKSYPGAGH  190
            YG   P    L E + G CP++AS+G +D    GAA +L        +P  +  YP AG 
Sbjct  140  YGQLPPKTEMLDEAMAGTCPLIASYGAKDRTLRGAAGKLEIALNKAGVPHQVTEYPQAGQ  199

Query  191  SFANKLPG-----QPLVRIAGFGYNEAATEDAWRRVFEFFGQHLR  230
            +F N  P      QPL+R+ G G        AWRRV ++F  +L+
Sbjct  200  AFLNDEPTGPKLIQPLIRVMGIGPEPEPAATAWRRVEDYFALYLK  244


>gi|258653114|ref|YP_003202270.1| dienelactone hydrolase [Nakamurella multipartita DSM 44233]
 gi|258556339|gb|ACV79281.1| dienelactone hydrolase [Nakamurella multipartita DSM 44233]
Length=275

 Score =  163 bits (412),  Expect = 2e-38, Method: Compositional matrix adjust.
 Identities = 92/222 (42%), Positives = 129/222 (59%), Gaps = 7/222 (3%)

Query  21   PGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPNMYARGGRARCITRVFRELLT  80
            P  GPWPGVVVVH+A G   D +   +  A AGY+ L P++Y      RC+    R+L  
Sbjct  49   PDPGPWPGVVVVHEATGPTDDMQEQCDWFAAAGYLALMPDLYHGRSMVRCVKGTVRQLFA  108

Query  81   KRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALVLSPR-GFGATAPFYGTPLPR  139
            +RG   D + +AR +L    +C+G VG+ G+C+GG FAL+L+ R G+ A A  YG PLP 
Sbjct  109  QRGPVFDQLESARAYLAGRDDCTGAVGVAGYCLGGGFALLLAGRGGYDAGAVNYG-PLPP  167

Query  140  HLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPADIKSYPGAGHSFANK----  195
            +L E L G+CP+VASFG RD    GA   L        +P D++ YP A H+F ++    
Sbjct  168  NLDEVLAGSCPLVASFGGRDRSLRGARATLTAAAERAGVPLDVREYPAARHAFISRNVVP  227

Query  196  LPGQPLVRIAGFGYNEAATEDAWRRVFEFFGQHLRAGSPGEP  237
             P   + ++ G GY+  +  +A RR+  FF +HLRA SPG P
Sbjct  228  SPLTTIGKVFGVGYDHDSAAEAKRRILAFFDEHLRA-SPGPP  268


>gi|116671815|ref|YP_832748.1| dienelactone hydrolase [Arthrobacter sp. FB24]
 gi|116611924|gb|ABK04648.1| dienelactone hydrolase [Arthrobacter sp. FB24]
Length=249

 Score =  154 bits (388),  Expect = 1e-35, Method: Compositional matrix adjust.
 Identities = 82/219 (38%), Positives = 127/219 (58%), Gaps = 6/219 (2%)

Query  17   LGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPNMYARGGRARCITRVFR  76
            L  PPG GP+P V+++H+A G    ++  ++R+A AGY+ L  ++++ GG  RCI    R
Sbjct  31   LAEPPGPGPFPAVLMIHEAFGLNDISRRQADRLAAAGYLTLAVDLFSNGGMRRCIVSTMR  90

Query  77   ELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALVLSPRGFGATAPFYGTP  136
             + +  G+A  D+  A+  L      +G+VG++GFCMGG FAL+L+ +GF A A  YG  
Sbjct  91   SMASGSGQAFTDLAIAQAWLEKSGRTNGKVGVIGFCMGGGFALLLANKGFDAAAVNYGR-  149

Query  137  LPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPADIKSYPGAGHSFANKL  196
            LP++  + L GACP+V +FG  D    GAA +L        I  DIK + GAGH+F N  
Sbjct  150  LPKNPEDALAGACPVVGNFGNADRSLPGAAAKLEATLENLGIEHDIKEFDGAGHAFMNDA  209

Query  197  PG-----QPLVRIAGFGYNEAATEDAWRRVFEFFGQHLR  230
                   +PL R+ G   +  A  +AW+R+ + F ++L+
Sbjct  210  EEGPRLLRPLFRVMGIKPDPDAAAEAWQRIEDHFARYLK  248


>gi|262200320|ref|YP_003271528.1| dienelactone hydrolase [Gordonia bronchialis DSM 43247]
 gi|262083667|gb|ACY19635.1| dienelactone hydrolase [Gordonia bronchialis DSM 43247]
Length=245

 Score =  144 bits (362),  Expect = 1e-32, Method: Compositional matrix adjust.
 Identities = 94/224 (42%), Positives = 120/224 (54%), Gaps = 10/224 (4%)

Query  4    IEIDAPAGPIDALLGLPPG-QGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPNMY  62
            I IDA     DAL+  P    GP P V++VHDA G   D +     +A  GY+ L P ++
Sbjct  10   ITIDAEPDSFDALIARPAEPSGPRPSVLLVHDATGDEVDTERNLRIVAGQGYLALAPLLF  69

Query  63   ARG-GRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALVL  121
              G  +  CI    R L    G A   +  AR  L    + +G V IVGFCMGG FAL+ 
Sbjct  70   TTGPNKLGCIVSAMRSLAVGHGPAFAILERARVTLADDAQSTGDVAIVGFCMGGGFALLE  129

Query  122  SPRGFGATAPFYG--TPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIP  179
                + A APFYG  T   R  ++T    CP+VASFG RDPL      RLR+V     I 
Sbjct  130  GDARYVAAAPFYGSLTSYRRVRADT----CPVVASFGQRDPLLPFGERRLRRVLDKHGIT  185

Query  180  ADIKSYPGAGHSFANKLPGQP--LVRIAGFGYNEAATEDAWRRV  221
            +DIK+YPG GHSFAN++   P  ++RIAG  Y+   + DAW RV
Sbjct  186  SDIKTYPGVGHSFANQIDAVPDRVLRIAGMHYDADVSADAWGRV  229


>gi|220913809|ref|YP_002489118.1| dienelactone hydrolase [Arthrobacter chlorophenolicus A6]
 gi|219860687|gb|ACL41029.1| dienelactone hydrolase [Arthrobacter chlorophenolicus A6]
Length=244

 Score =  141 bits (356),  Expect = 6e-32, Method: Compositional matrix adjust.
 Identities = 88/222 (40%), Positives = 127/222 (58%), Gaps = 9/222 (4%)

Query  17   LGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPNMYARGGRARCITRVFR  76
            L +P G+GP+PGVVV+H+A G     +  ++R+A AGY+ L  ++Y+  G  RC+    R
Sbjct  23   LAVPEGEGPFPGVVVIHEAFGLDDQTRRHADRLALAGYLALALDLYSDVGARRCLVGTMR  82

Query  77   ELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALVLSPRGFGATAPFYGTP  136
             +  + GR   DI  AR  L      +GR G++GFCMGG FAL+ +  GF A +  YG  
Sbjct  83   AMAARTGRPFTDIATARTWLANSDLSNGRTGVIGFCMGGGFALLAARDGFQAASVNYGR-  141

Query  137  LPRH---LSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPADIKSYPGAGHSFA  193
            LP+    L E L GACPIVA++G  D    GAA RL        I   +K +PGAGHSF 
Sbjct  142  LPKEQEDLVEALRGACPIVANYGGADRSLKGAAARLDSALDQLGIEHSVKEFPGAGHSFL  201

Query  194  NKLPG-----QPLVRIAGFGYNEAATEDAWRRVFEFFGQHLR  230
            N         +PL+R+ G G +  +  +AWRR+ ++F ++L+
Sbjct  202  NDEEMGPALLRPLMRVMGVGPDPESAPEAWRRIEDYFARYLK  243


>gi|325001844|ref|ZP_08122956.1| carboxymethylenebutenolidase [Pseudonocardia sp. P1]
Length=236

 Score =  135 bits (341),  Expect = 4e-30, Method: Compositional matrix adjust.
 Identities = 91/244 (38%), Positives = 121/244 (50%), Gaps = 26/244 (10%)

Query  3    TIEIDAPA-----GPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVL  57
            T+E D        G +   L  P    P P +V++++A G  P+ + I+  +AR GY VL
Sbjct  2    TVEADVAVPSVNGGTLPGFLARPETGEPGPALVMIYEAFGMTPEMRRIARELAREGYTVL  61

Query  58   TPNMYARGG-RARCITRVFRELLTKRGRALDDILAARDHLLAMPECSG-RVGIVGFCMGG  115
             P+++ARG  +  C+      + + RG AL D  +AR  L   P   G R+G +GFCMGG
Sbjct  62   IPDLFARGKVKPLCVASTMATMASGRGAALGDCESARRWLAGQPTVDGDRIGAIGFCMGG  121

Query  116  QFALVLSPRG-FGATAPFYGTPLPRHLSETLNGACPIVASFGTRD-PLGIGAANRLRKVT  173
             FA++LS  G +    PFYG    R     +   CP+VASFG RD     G   RL    
Sbjct  122  GFAMLLSQTGLYRVAVPFYGRSGTR-----VEPVCPVVASFGGRDAQFADGYPERLEADL  176

Query  174  AAKNIPADIKSYPGAGHSFANKLPG--------QPLVRIAGFGYNEAATEDAWRRVFEFF  225
            A + IP D+K YP AGHSF  +  G         PL       Y+E A  DA RRV EF 
Sbjct  177  AERGIPHDVKVYPDAGHSFMTRTEGVLGAAARRSPL----HAAYDEPAATDATRRVLEFL  232

Query  226  GQHL  229
              HL
Sbjct  233  RTHL  236


>gi|184201845|ref|YP_001856052.1| hypothetical protein KRH_21990 [Kocuria rhizophila DC2201]
 gi|183582075|dbj|BAG30546.1| hypothetical protein [Kocuria rhizophila DC2201]
Length=192

 Score =  131 bits (329),  Expect = 9e-29, Method: Compositional matrix adjust.
 Identities = 79/189 (42%), Positives = 103/189 (55%), Gaps = 11/189 (5%)

Query  51   RAGYVVLTPNMYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVG  110
            R G     P++Y+ GG  RC+    R L+T  G+AL DI  +R  LLA PE +  VGI+G
Sbjct  9    RVGIRDRRPDLYSDGGARRCLVATMRSLMTGTGKALVDIETSRQWLLAQPETTEAVGIIG  68

Query  111  FCMGGQFALVLSPRGFGATAPF-YGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRL  169
            FCMGG FAL+   R   A A   YGT     + E +  ACP V S+G RD    GAA +L
Sbjct  69   FCMGGGFALLTCDRDRYAVASVNYGT-----VPEDVGHACPAVGSYGARDLQNRGAAQKL  123

Query  170  RKVTAAKNIPADIKSYPGAGHSFAN-KLPG----QPLVRIAGFGYNEAATEDAWRRVFEF  224
                 A ++  DI+ YP AGH+F N  +PG      L  +AGFG      E AWRR+ ++
Sbjct  124  EAKLDAASVGYDIQEYPSAGHAFLNDSMPGPAALASLWHVAGFGGTREDREHAWRRIEDY  183

Query  225  FGQHLRAGS  233
            F  HL A +
Sbjct  184  FAAHLGASA  192


>gi|336117290|ref|YP_004572057.1| putative hydrolase [Microlunatus phosphovorus NM-1]
 gi|334685069|dbj|BAK34654.1| putative hydrolase [Microlunatus phosphovorus NM-1]
Length=234

 Score =  129 bits (323),  Expect = 5e-28, Method: Compositional matrix adjust.
 Identities = 82/235 (35%), Positives = 125/235 (54%), Gaps = 7/235 (2%)

Query  1    MTTIEIDAPAGPIDALLGLPPGQGPWPGVVVVHDAVGYVPDNKLISERIARAGYVVLTPN  60
            M T+  D   G   A+   P G G WPGVV++H+  G     +  ++R+A AGY+V+ P+
Sbjct  1    METVTEDVTIGDRMAVSARPAGAGRWPGVVMLHEGYGINEVLRRQAQRLASAGYLVVAPD  60

Query  61   MYARGGRARCITRVFRELLTKRGRALDDILAARDHLLAMPECSGRVGIVGFCMGGQFALV  120
            +   G R   +      L  ++G   + I  +R  ++   EC+G+VG++GF +GG +AL 
Sbjct  61   LLGDGQRYDALKSALESLTARQGTPFEVIAQSRTWVIDRRECNGKVGVIGFGLGGGYALA  120

Query  121  LSPRGFGATAPFYGTPLPRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPA  180
            ++  GF A A  Y   +P    E L G+CPIV S+G +D L  G   +L        I  
Sbjct  121  VAGSGFDAAAVNYAV-IPEDAIELLRGSCPIVGSYGAKDRLS-GGITKLAAALEEHQIDY  178

Query  181  DIKSYPGAGHSFANKLPG-----QPLVRIAGFGYNEAATEDAWRRVFEFFGQHLR  230
            D+K Y  AGHSF N +P      +P+++IA  G    + + AW R+  FFG+HLR
Sbjct  179  DLKVYRRAGHSFLNDVPVGPVLLRPMMKIASVGPEPESAKHAWGRIESFFGRHLR  233


>gi|254429403|ref|ZP_05043110.1| dienelactone hydrolase family [Alcanivorax sp. DG881]
 gi|196195572|gb|EDX90531.1| dienelactone hydrolase family [Alcanivorax sp. DG881]
Length=249

 Score =  123 bits (308),  Expect = 2e-26, Method: Compositional matrix adjust.
 Identities = 77/208 (38%), Positives = 113/208 (55%), Gaps = 16/208 (7%)

Query  29   VVVVHDAVGYVPDNKLISERIARAGYVVLTPNMYAR-GGRARCITRVFRELLTKRGRALD  87
            V+ +HD  GY  D + I++R+A  GY VL P++Y   G +A C+ +  +   T +G A  
Sbjct  33   VIAIHDIFGYTDDIERIAQRLADNGYPVLVPDLYDLPGSKALCVVKTLKAHETGKGPAFA  92

Query  88   DILAARDHLLAMPEC-SGRVGIVGFCMGGQFALVLSPRG-FGATAPFYGTPLPRHLSETL  145
             + A R+ LLA  E    +VG++GFCMGG+FA++ + +      APFYG  +P+  +ETL
Sbjct  93   QLEACREWLLAQSEEPVTQVGVMGFCMGGRFAVLYASQAPVQVVAPFYGG-IPKK-AETL  150

Query  146  NGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPADIKSYPGAGHSFAN--------KLP  197
             G CP V  +G +D L      RL        +P DI+SY   GHSF N        KL 
Sbjct  151  RGICPAVGGWGKQDMLYGDHGERLASHLETLGVPHDIRSYDDVGHSFMNDHQTFTFKKLG  210

Query  198  GQPLVRIAGFGYNEAATEDAWRRVFEFF  225
              P +R     ++  A+ED+W+RV  FF
Sbjct  211  PLPPLRSK---FDAGASEDSWQRVLAFF  235


>gi|110834479|ref|YP_693338.1| dienelactone hydrolase family protein [Alcanivorax borkumensis 
SK2]
 gi|110647590|emb|CAL17066.1| dienelactone hydrolase family protein [Alcanivorax borkumensis 
SK2]
Length=247

 Score =  123 bits (308),  Expect = 3e-26, Method: Compositional matrix adjust.
 Identities = 80/216 (38%), Positives = 115/216 (54%), Gaps = 19/216 (8%)

Query  24   GPWP---GVVVVHDAVGYVPDNKLISERIARAGYVVLTPNMYAR-GGRARCITRVFRELL  79
            GP P    V+ +HD  GY  D + I+ R+A  GY VL P++Y   G +A C+ +  +   
Sbjct  25   GPQPHKAAVIAIHDIFGYTDDIERIATRLADNGYPVLVPDLYDLPGSKALCVVKTLKAHE  84

Query  80   TKRGRALDDILAARDHLLAMP-ECSGRVGIVGFCMGGQFALVLSPRG-FGATAPFYGTPL  137
            T +G A   + A R  LLA   E   +VG++GFCMGG+FA++ + +      APFYG  +
Sbjct  85   TGKGTAFVQLEACRKWLLAQTDEPVSQVGVMGFCMGGRFAVLYASQAPVQVVAPFYGG-I  143

Query  138  PRHLSETLNGACPIVASFGTRDPLGIGAANRLRKVTAAKNIPADIKSYPGAGHSFAN---  194
            P+  +ETL G CP V  +G +D L      RL     +  +P DI+SY   GHSF N   
Sbjct  144  PKK-AETLRGICPAVGGWGKQDMLFGDHGERLASHMESLGVPHDIRSYDDVGHSFMNDHQ  202

Query  195  -----KLPGQPLVRIAGFGYNEAATEDAWRRVFEFF  225
                 KL   P +R     ++  A+ED+W+RV  FF
Sbjct  203  TFTFKKLGPLPPLRSK---FDAGASEDSWQRVLAFF  235



Lambda     K      H
   0.322    0.142    0.445 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Effective search space used: 322714888716


  Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
    Posted date:  Sep 5, 2011  4:36 AM
  Number of letters in database: 5,219,829,388
  Number of sequences in database:  15,229,318



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40