BLASTP 2.2.25+
Reference:
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database
search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
15,229,318 sequences; 5,219,829,388 total letters
Query= Rv2128
Length=67
Score E
Sequences producing significant alignments: (Bits) Value
gi|15609265|ref|NP_216644.1| transmembrane protein [Mycobacteriu... 131 3e-29
gi|333988975|ref|YP_004521589.1| transmembrane protein [Mycobact... 80.9 6e-14
gi|118616243|ref|YP_904575.1| hypothetical protein MUL_0384 [Myc... 62.4 2e-08
gi|326334043|ref|ZP_08200272.1| hypothetical protein NBCG_05476 ... 49.7 1e-04
gi|183984784|ref|YP_001853075.1| hypothetical protein MMAR_4816 ... 43.5 0.009
gi|254502139|ref|ZP_05114290.1| putative transporter, CPA2 famil... 40.4 0.095
gi|118590724|ref|ZP_01548125.1| probable glutathione-regulated p... 39.7 0.15
gi|328545017|ref|YP_004305126.1| glutathione-regulated potassium... 38.1 0.39
gi|307941670|ref|ZP_07657025.1| glutathione-regulated potassium-... 37.7 0.53
gi|118471535|ref|YP_887036.1| hypothetical protein MSMEG_2701 [M... 35.8 1.9
gi|227821000|ref|YP_002824970.1| glutathione-regulated potassium... 34.7 5.3
gi|108757577|ref|YP_635069.1| glutathione-regulated potassium-ef... 33.5 9.6
>gi|15609265|ref|NP_216644.1| transmembrane protein [Mycobacterium tuberculosis H37Rv]
gi|31793308|ref|NP_855801.1| transmembrane protein [Mycobacterium bovis AF2122/97]
gi|121638010|ref|YP_978234.1| putative transmembrane protein [Mycobacterium bovis BCG str.
Pasteur 1173P2]
72 more sequence titles
Length=67
Score = 131 bits (330), Expect = 3e-29, Method: Compositional matrix adjust.
Identities = 67/67 (100%), Positives = 67/67 (100%), Gaps = 0/67 (0%)
Query 1 MLRRGESIIRNRYASKPPLYGMAMVFLAMAVVAVTAYFRMGWWSIIGYAAAAIIGVIGFA 60
MLRRGESIIRNRYASKPPLYGMAMVFLAMAVVAVTAYFRMGWWSIIGYAAAAIIGVIGFA
Sbjct 1 MLRRGESIIRNRYASKPPLYGMAMVFLAMAVVAVTAYFRMGWWSIIGYAAAAIIGVIGFA 60
Query 61 LAFRDLS 67
LAFRDLS
Sbjct 61 LAFRDLS 67
>gi|333988975|ref|YP_004521589.1| transmembrane protein [Mycobacterium sp. JDM601]
gi|333484943|gb|AEF34335.1| transmembrane protein [Mycobacterium sp. JDM601]
Length=60
Score = 80.9 bits (198), Expect = 6e-14, Method: Compositional matrix adjust.
Identities = 39/57 (69%), Positives = 45/57 (79%), Gaps = 0/57 (0%)
Query 11 NRYASKPPLYGMAMVFLAMAVVAVTAYFRMGWWSIIGYAAAAIIGVIGFALAFRDLS 67
NRYA+KPPLYGM +VF A+A+VAVTAY GWWS++GYA AAI V G LAFRD S
Sbjct 4 NRYAAKPPLYGMVLVFCAIAIVAVTAYLHGGWWSVLGYAFAAITAVAGGVLAFRDFS 60
>gi|118616243|ref|YP_904575.1| hypothetical protein MUL_0384 [Mycobacterium ulcerans Agy99]
gi|118568353|gb|ABL03104.1| conserved hypothetical transmembrane protein [Mycobacterium ulcerans
Agy99]
Length=80
Score = 62.4 bits (150), Expect = 2e-08, Method: Compositional matrix adjust.
Identities = 31/56 (56%), Positives = 38/56 (68%), Gaps = 0/56 (0%)
Query 12 RYASKPPLYGMAMVFLAMAVVAVTAYFRMGWWSIIGYAAAAIIGVIGFALAFRDLS 67
+ +K PL GM+MVF AMA+VAVTAY GW+SIIGY AA + GF FRD+
Sbjct 5 DFKAKTPLIGMSMVFGAMALVAVTAYLHAGWYSIIGYVIAAAVAAFGFIFTFRDIP 60
>gi|326334043|ref|ZP_08200272.1| hypothetical protein NBCG_05476 [Nocardioidaceae bacterium Broad-1]
gi|325948192|gb|EGD40303.1| hypothetical protein NBCG_05476 [Nocardioidaceae bacterium Broad-1]
Length=74
Score = 49.7 bits (117), Expect = 1e-04, Method: Compositional matrix adjust.
Identities = 33/61 (55%), Positives = 46/61 (76%), Gaps = 0/61 (0%)
Query 7 SIIRNRYASKPPLYGMAMVFLAMAVVAVTAYFRMGWWSIIGYAAAAIIGVIGFALAFRDL 66
++ R+ Y + P+ GM MVFLA+A+V VTAY +GWWS+IGYAAAA+I V+G L F+D+
Sbjct 3 ALARDPYRPRTPIIGMVMVFLALAIVGVTAYLHLGWWSLIGYAAAAVIAVVGGWLLFKDV 62
Query 67 S 67
Sbjct 63 P 63
>gi|183984784|ref|YP_001853075.1| hypothetical protein MMAR_4816 [Mycobacterium marinum M]
gi|183178110|gb|ACC43220.1| conserved hypothetical transmembrane protein [Mycobacterium marinum
M]
Length=80
Score = 43.5 bits (101), Expect = 0.009, Method: Compositional matrix adjust.
Identities = 32/55 (59%), Positives = 39/55 (71%), Gaps = 0/55 (0%)
Query 13 YASKPPLYGMAMVFLAMAVVAVTAYFRMGWWSIIGYAAAAIIGVIGFALAFRDLS 67
+ +K PL GM+MVF AMA+VAVTAY GW+SIIGYA AA + GF FRD+
Sbjct 6 FKAKTPLIGMSMVFGAMALVAVTAYLHAGWYSIIGYAIAAAVAAFGFIFTFRDIP 60
>gi|254502139|ref|ZP_05114290.1| putative transporter, CPA2 family [Labrenzia alexandrii DFL-11]
gi|222438210|gb|EEE44889.1| putative transporter, CPA2 family [Labrenzia alexandrii DFL-11]
Length=596
Score = 40.4 bits (93), Expect = 0.095, Method: Compositional matrix adjust.
Identities = 23/48 (48%), Positives = 30/48 (63%), Gaps = 1/48 (2%)
Query 15 SKPPLYGMAMVFLAMAVVAVTAYFRMGWWSIIGY-AAAAIIGVIGFAL 61
S PPL+ A++FL+ VVAV R+G S++GY AA A IG G L
Sbjct 5 SAPPLFAEAIIFLSATVVAVPIAKRLGLGSVVGYLAAGAAIGPHGLQL 52
>gi|118590724|ref|ZP_01548125.1| probable glutathione-regulated potassium-efflux system transmembrane
protein [Stappia aggregata IAM 12614]
gi|118436700|gb|EAV43340.1| probable glutathione-regulated potassium-efflux system transmembrane
protein [Stappia aggregata IAM 12614]
Length=597
Score = 39.7 bits (91), Expect = 0.15, Method: Compositional matrix adjust.
Identities = 22/46 (48%), Positives = 29/46 (64%), Gaps = 1/46 (2%)
Query 17 PPLYGMAMVFLAMAVVAVTAYFRMGWWSIIGY-AAAAIIGVIGFAL 61
PP + ++VFLA VVAV R+G S++GY AA A+IG G L
Sbjct 7 PPFFAESIVFLAATVVAVPIAKRLGLGSVVGYLAAGAVIGPHGLKL 52
>gi|328545017|ref|YP_004305126.1| glutathione-regulated potassium-efflux system transmembrane protein
[Polymorphum gilvum SL003B-26A1]
gi|326414759|gb|ADZ71822.1| Probable glutathione-regulated potassium-efflux system transmembrane
protein [Polymorphum gilvum SL003B-26A1]
Length=591
Score = 38.1 bits (87), Expect = 0.39, Method: Compositional matrix adjust.
Identities = 18/37 (49%), Positives = 25/37 (68%), Gaps = 0/37 (0%)
Query 17 PPLYGMAMVFLAMAVVAVTAYFRMGWWSIIGYAAAAI 53
PPL+ A++FLA VVAV R+G S++GY AA +
Sbjct 7 PPLFTEAIIFLAATVVAVPLAKRLGLGSVVGYLAAGV 43
>gi|307941670|ref|ZP_07657025.1| glutathione-regulated potassium-efflux system protein KefB [Roseibium
sp. TrichSKD4]
gi|307775278|gb|EFO34484.1| glutathione-regulated potassium-efflux system protein KefB [Roseibium
sp. TrichSKD4]
Length=570
Score = 37.7 bits (86), Expect = 0.53, Method: Compositional matrix adjust.
Identities = 21/46 (46%), Positives = 28/46 (61%), Gaps = 1/46 (2%)
Query 15 SKPPLYGMAMVFLAMAVVAVTAYFRMGWWSIIGY-AAAAIIGVIGF 59
+ PP + ++VFLA VVAV R+G S++GY AA A IG G
Sbjct 5 TAPPFFAESIVFLAATVVAVPIAKRLGLGSVVGYLAAGAAIGPFGL 50
>gi|118471535|ref|YP_887036.1| hypothetical protein MSMEG_2701 [Mycobacterium smegmatis str.
MC2 155]
gi|118172822|gb|ABK73718.1| hypothetical protein MSMEG_2701 [Mycobacterium smegmatis str.
MC2 155]
Length=67
Score = 35.8 bits (81), Expect = 1.9, Method: Compositional matrix adjust.
Identities = 20/53 (38%), Positives = 30/53 (57%), Gaps = 0/53 (0%)
Query 13 YASKPPLYGMAMVFLAMAVVAVTAYFRMGWWSIIGYAAAAIIGVIGFALAFRD 65
+ + PLYG+ +V A+ + +TA GW ++GY A IGV GF + RD
Sbjct 6 HPQRTPLYGVLLVVAAVLLCWLTASAFTGWQMVVGYLLAVAIGVFGFVMTLRD 58
>gi|227821000|ref|YP_002824970.1| glutathione-regulated potassium-efflux system protein [Sinorhizobium
fredii NGR234]
gi|227339999|gb|ACP24217.1| glutathione-regulated potassium-efflux system protein [Sinorhizobium
fredii NGR234]
Length=618
Score = 34.7 bits (78), Expect = 5.3, Method: Compositional matrix adjust.
Identities = 17/40 (43%), Positives = 25/40 (63%), Gaps = 0/40 (0%)
Query 15 SKPPLYGMAMVFLAMAVVAVTAYFRMGWWSIIGYAAAAII 54
+ P LY A++ L AVVA + R+G +I+GY AA I+
Sbjct 3 TTPILYTQALMLLGGAVVAAPLFKRLGLGTILGYLAAGIV 42
>gi|108757577|ref|YP_635069.1| glutathione-regulated potassium-efflux system [Myxococcus xanthus
DK 1622]
gi|108461457|gb|ABF86642.1| glutathione-regulated potassium-efflux system [Myxococcus xanthus
DK 1622]
Length=595
Score = 33.5 bits (75), Expect = 9.6, Method: Compositional matrix adjust.
Identities = 21/41 (52%), Positives = 27/41 (66%), Gaps = 1/41 (2%)
Query 22 MAMVFLAMAVVAVTAYFRMGWWSIIGY-AAAAIIGVIGFAL 61
A+VFLA AV+AV R+G S++GY AA A+IG G L
Sbjct 6 QALVFLAAAVLAVPLSRRLGLGSVLGYLAAGAVIGPWGLKL 46
Lambda K H
0.334 0.141 0.441
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 129524807608
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Sep 5, 2011 4:36 AM
Number of letters in database: 5,219,829,388
Number of sequences in database: 15,229,318
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40