BLASTP 2.2.25+
Reference:
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database
search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
15,229,318 sequences; 5,219,829,388 total letters
Query= Rv2297
Length=150
Score E
Sequences producing significant alignments: (Bits) Value
gi|15609434|ref|NP_216813.1| hypothetical protein Rv2297 [Mycoba... 309 8e-83
gi|340627305|ref|YP_004745757.1| hypothetical protein MCAN_23231... 308 2e-82
gi|254365077|ref|ZP_04981123.1| hypothetical protein TBHG_02235 ... 306 9e-82
gi|313116830|ref|YP_004037954.1| hypothetical protein Hbor_32880... 38.5 0.37
gi|20090229|ref|NP_616304.1| thioredoxin reductase (NADPH) [Meth... 34.3 5.7
gi|343427455|emb|CBQ70982.1| related to phosphatidic acid-prefer... 34.3 5.8
>gi|15609434|ref|NP_216813.1| hypothetical protein Rv2297 [Mycobacterium tuberculosis H37Rv]
gi|15841788|ref|NP_336825.1| hypothetical protein MT2354 [Mycobacterium tuberculosis CDC1551]
gi|31793475|ref|NP_855968.1| hypothetical protein Mb2319 [Mycobacterium bovis AF2122/97]
77 more sequence titles
Length=150
Score = 309 bits (792), Expect = 8e-83, Method: Compositional matrix adjust.
Identities = 150/150 (100%), Positives = 150/150 (100%), Gaps = 0/150 (0%)
Query 1 MAMEMAMMGLLGTVVGASAMGIGGIAKSIAEAYVPGVAAAKDRRQQMNVDLQARRYEAVR 60
MAMEMAMMGLLGTVVGASAMGIGGIAKSIAEAYVPGVAAAKDRRQQMNVDLQARRYEAVR
Sbjct 1 MAMEMAMMGLLGTVVGASAMGIGGIAKSIAEAYVPGVAAAKDRRQQMNVDLQARRYEAVR 60
Query 61 VWRSGLCSASNAYRQWEAGSRDTHAPNVVGDEWFEGLRPHLPTTGEAAKFRTAYEVRCDN 120
VWRSGLCSASNAYRQWEAGSRDTHAPNVVGDEWFEGLRPHLPTTGEAAKFRTAYEVRCDN
Sbjct 61 VWRSGLCSASNAYRQWEAGSRDTHAPNVVGDEWFEGLRPHLPTTGEAAKFRTAYEVRCDN 120
Query 121 PTLMVLSLEIGRIEKEWMVEASGRTPKHRG 150
PTLMVLSLEIGRIEKEWMVEASGRTPKHRG
Sbjct 121 PTLMVLSLEIGRIEKEWMVEASGRTPKHRG 150
>gi|340627305|ref|YP_004745757.1| hypothetical protein MCAN_23231 [Mycobacterium canettii CIPT
140010059]
gi|340005495|emb|CCC44655.1| hypothetical protein MCAN_23231 [Mycobacterium canettii CIPT
140010059]
Length=150
Score = 308 bits (788), Expect = 2e-82, Method: Compositional matrix adjust.
Identities = 149/150 (99%), Positives = 149/150 (99%), Gaps = 0/150 (0%)
Query 1 MAMEMAMMGLLGTVVGASAMGIGGIAKSIAEAYVPGVAAAKDRRQQMNVDLQARRYEAVR 60
MAMEMAMMGLLGTVVGASAMGIGGIAKSIAEAYVPGVAAAKDRRQQMNVDLQARRYEAVR
Sbjct 1 MAMEMAMMGLLGTVVGASAMGIGGIAKSIAEAYVPGVAAAKDRRQQMNVDLQARRYEAVR 60
Query 61 VWRSGLCSASNAYRQWEAGSRDTHAPNVVGDEWFEGLRPHLPTTGEAAKFRTAYEVRCDN 120
VWRSGLCSASNAYRQWEAGSRDTHAPNVVGDEWFEGLRPHLPTTGEAAKFRTAYEVRCDN
Sbjct 61 VWRSGLCSASNAYRQWEAGSRDTHAPNVVGDEWFEGLRPHLPTTGEAAKFRTAYEVRCDN 120
Query 121 PTLMVLSLEIGRIEKEWMVEASGRTPKHRG 150
PTLMVLSLEIGRIEKEWM EASGRTPKHRG
Sbjct 121 PTLMVLSLEIGRIEKEWMAEASGRTPKHRG 150
>gi|254365077|ref|ZP_04981123.1| hypothetical protein TBHG_02235 [Mycobacterium tuberculosis str.
Haarlem]
gi|134150591|gb|EBA42636.1| hypothetical protein TBHG_02235 [Mycobacterium tuberculosis str.
Haarlem]
Length=148
Score = 306 bits (783), Expect = 9e-82, Method: Compositional matrix adjust.
Identities = 148/148 (100%), Positives = 148/148 (100%), Gaps = 0/148 (0%)
Query 3 MEMAMMGLLGTVVGASAMGIGGIAKSIAEAYVPGVAAAKDRRQQMNVDLQARRYEAVRVW 62
MEMAMMGLLGTVVGASAMGIGGIAKSIAEAYVPGVAAAKDRRQQMNVDLQARRYEAVRVW
Sbjct 1 MEMAMMGLLGTVVGASAMGIGGIAKSIAEAYVPGVAAAKDRRQQMNVDLQARRYEAVRVW 60
Query 63 RSGLCSASNAYRQWEAGSRDTHAPNVVGDEWFEGLRPHLPTTGEAAKFRTAYEVRCDNPT 122
RSGLCSASNAYRQWEAGSRDTHAPNVVGDEWFEGLRPHLPTTGEAAKFRTAYEVRCDNPT
Sbjct 61 RSGLCSASNAYRQWEAGSRDTHAPNVVGDEWFEGLRPHLPTTGEAAKFRTAYEVRCDNPT 120
Query 123 LMVLSLEIGRIEKEWMVEASGRTPKHRG 150
LMVLSLEIGRIEKEWMVEASGRTPKHRG
Sbjct 121 LMVLSLEIGRIEKEWMVEASGRTPKHRG 148
>gi|313116830|ref|YP_004037954.1| hypothetical protein Hbor_32880 [Halogeometricum borinquense
DSM 11551]
gi|312294782|gb|ADQ68818.1| hypothetical protein Hbor_32880 [Halogeometricum borinquense
DSM 11551]
Length=754
Score = 38.5 bits (88), Expect = 0.37, Method: Composition-based stats.
Identities = 24/70 (35%), Positives = 32/70 (46%), Gaps = 0/70 (0%)
Query 81 RDTHAPNVVGDEWFEGLRPHLPTTGEAAKFRTAYEVRCDNPTLMVLSLEIGRIEKEWMVE 140
R+T + GD+ GLRP L + E K +E DN L +EI IE + +E
Sbjct 265 RETRYSSSWGDQHTRGLRPRLNESPEVVKDVDKFEHTFDNDVLGTKQIEIYLIESKKELE 324
Query 141 ASGRTPKHRG 150
G P RG
Sbjct 325 ERGINPDRRG 334
>gi|20090229|ref|NP_616304.1| thioredoxin reductase (NADPH) [Methanosarcina acetivorans C2A]
gi|19915221|gb|AAM04784.1| thioredoxin reductase (NADPH) [Methanosarcina acetivorans C2A]
Length=315
Score = 34.3 bits (77), Expect = 5.7, Method: Compositional matrix adjust.
Identities = 20/65 (31%), Positives = 30/65 (47%), Gaps = 1/65 (1%)
Query 86 PNVVGDEWFEGLRPHLPTTGEAAKFRTAYEVRCDNPTLMVLSLEIGRIEKEWMVEASGRT 145
P++ G E E R H G K VR + +V S + G +E + ++ A+G
Sbjct 62 PSISGLELMEKYRTHAQEVGVKTKITEVLSVRTEGAKKIV-STDSGDLEAKTLIIATGAN 120
Query 146 PKHRG 150
PKH G
Sbjct 121 PKHLG 125
>gi|343427455|emb|CBQ70982.1| related to phosphatidic acid-preferring phospholipase A1 [Sporisorium
reilianum SRZ2]
Length=983
Score = 34.3 bits (77), Expect = 5.8, Method: Composition-based stats.
Identities = 16/50 (32%), Positives = 28/50 (56%), Gaps = 2/50 (4%)
Query 57 EAVRVWRSGLCSASNAYRQWEAGSRDTHAPNVVGDEWFEGLRPHLPTTGE 106
EA++ SG +S+ +Q ++ ++ H P D WF GLR ++P G+
Sbjct 81 EAIQSALSGFADSSHPNKQSKSSAKSDHKP--AKDSWFGGLRQYIPFAGK 128
Lambda K H
0.318 0.131 0.399
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 129459908798
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Sep 5, 2011 4:36 AM
Number of letters in database: 5,219,829,388
Number of sequences in database: 15,229,318
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40