BLASTP 2.2.25+


Reference:
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database
search programs", Nucleic Acids Res. 25:3389-3402.



Reference for composition-based statistics:
Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.



Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
           15,229,318 sequences; 5,219,829,388 total letters



Query= Rv2385

Length=306
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

gi|15841897|ref|NP_336934.1|  esterase, putative [Mycobacterium t...   615    2e-174
gi|31793562|ref|NP_856055.1|  putative acetyl hydrolase MBTJ [Myc...   613    9e-174
gi|340627396|ref|YP_004745848.1|  putative acetyl hydrolase MBTJ ...   611    4e-173
gi|254365160|ref|ZP_04981206.1|  acetyl hydrolase mbtJ [Mycobacte...   611    4e-173
gi|240169930|ref|ZP_04748589.1|  acetyl hydrolase MbtJ [Mycobacte...   434    7e-120
gi|183983679|ref|YP_001851970.1|  acetyl hydrolase MbtJ [Mycobact...   425    4e-117
gi|118618914|ref|YP_907246.1|  acetyl hydrolase MbtJ [Mycobacteri...   423    1e-116
gi|254822292|ref|ZP_05227293.1|  LipK [Mycobacterium intracellula...   419    3e-115
gi|254774614|ref|ZP_05216130.1|  LipK [Mycobacterium avium subsp....   416    2e-114
gi|41408295|ref|NP_961131.1|  LipK [Mycobacterium avium subsp. pa...   414    1e-113
gi|336458109|gb|EGO37092.1|  esterase/lipase [Mycobacterium avium...   404    1e-110
gi|118464610|ref|YP_881022.1|  alpha/beta hydrolase [Mycobacteriu...   403    2e-110
gi|342857781|ref|ZP_08714437.1|  alpha/beta hydrolase [Mycobacter...   402    3e-110
gi|311743425|ref|ZP_07717231.1|  alpha/beta hydrolase domain prot...   267    2e-69 
gi|333920083|ref|YP_004493664.1|  alpha/beta hydrolase fold-3 dom...   264    1e-68 
gi|296141191|ref|YP_003648434.1|  alpha/beta hydrolase fold-3 pro...   258    7e-67 
gi|169631131|ref|YP_001704780.1|  putative lipase/esterase [Mycob...   247    2e-63 
gi|333990246|ref|YP_004522860.1|  acetyl hydrolase MbtJ [Mycobact...   246    5e-63 
gi|262200152|ref|YP_003271360.1|  alpha/beta hydrolase [Gordonia ...   211    1e-52 
gi|262203681|ref|YP_003274889.1|  alpha/beta hydrolase [Gordonia ...   207    1e-51 
gi|296169339|ref|ZP_06850967.1|  alpha/beta hydrolase domain prot...   199    5e-49 
gi|343926313|ref|ZP_08765822.1|  putative esterase [Gordonia alka...   199    6e-49 
gi|326383978|ref|ZP_08205662.1|  alpha/beta hydrolase fold-3 doma...   192    6e-47 
gi|145225184|ref|YP_001135862.1|  alpha/beta hydrolase domain-con...   191    2e-46 
gi|169629162|ref|YP_001702811.1|  putative esterase/acetyl hydrol...   187    1e-45 
gi|108800159|ref|YP_640356.1|  alpha/beta hydrolase fold-3 [Mycob...   187    2e-45 
gi|325001073|ref|ZP_08122185.1|  putative lipase/esterase [Pseudo...   172    5e-41 
gi|183983135|ref|YP_001851426.1|  esterase/lipase [Mycobacterium ...   161    9e-38 
gi|269128648|ref|YP_003302018.1|  alpha/beta hydrolase fold-3 dom...   159    7e-37 
gi|319948633|ref|ZP_08022757.1|  Alpha/beta hydrolase fold-3 doma...   158    1e-36 
gi|333919971|ref|YP_004493552.1|  alpha/beta hydrolase domain-con...   151    1e-34 
gi|295675448|ref|YP_003603972.1|  Alpha/beta hydrolase fold-3 dom...   149    5e-34 
gi|187922642|ref|YP_001894284.1|  alpha/beta hydrolase fold-3 dom...   148    8e-34 
gi|269126738|ref|YP_003300108.1|  alpha/beta hydrolase fold-3 dom...   139    4e-31 
gi|296161587|ref|ZP_06844392.1|  Alpha/beta hydrolase fold-3 doma...   139    5e-31 
gi|219957628|gb|ACL67845.1|  lipolytic enzyme [uncultured bacterium]   139    6e-31 
gi|223365892|pdb|3FAK|A  Chain A, Structural And Functional Analy...   137    2e-30 
gi|112434063|gb|ABI18351.1|  esterase/lipase [uncultured bacterium]    137    2e-30 
gi|154251800|ref|YP_001412624.1|  alpha/beta hydrolase domain-con...   137    2e-30 
gi|167561564|ref|ZP_02354480.1|  esterase, putative [Burkholderia...   136    3e-30 
gi|219957636|gb|ACL67849.1|  lipolytic enzyme [uncultured bacterium]   136    4e-30 
gi|91781781|ref|YP_556987.1|  putative lipase/esterase [Burkholde...   136    5e-30 
gi|307728425|ref|YP_003905649.1|  putative lipase/esterase [Burkh...   135    5e-30 
gi|167568797|ref|ZP_02361671.1|  esterase, putative [Burkholderia...   135    1e-29 
gi|115352848|ref|YP_774687.1|  alpha/beta hydrolase domain-contai...   134    2e-29 
gi|60892969|gb|AAX37295.1|  lipase/esterase [uncultured bacterium]     134    2e-29 
gi|170699668|ref|ZP_02890705.1|  Alpha/beta hydrolase fold-3 doma...   134    2e-29 
gi|171319380|ref|ZP_02908489.1|  Alpha/beta hydrolase fold-3 doma...   134    2e-29 
gi|45775276|gb|AAS77236.1|  lipase/esterase [uncultured bacterium]     132    6e-29 
gi|154251326|ref|YP_001412150.1|  alpha/beta hydrolase domain-con...   132    7e-29 


>gi|15841897|ref|NP_336934.1| esterase, putative [Mycobacterium tuberculosis CDC1551]
 gi|253798538|ref|YP_003031539.1| acetyl hydrolase mbtJ [Mycobacterium tuberculosis KZN 1435]
 gi|254232521|ref|ZP_04925848.1| acetyl hydrolase mbtJ [Mycobacterium tuberculosis C]
 gi|13882166|gb|AAK46748.1| esterase, putative [Mycobacterium tuberculosis CDC1551]
 gi|124601580|gb|EAY60590.1| acetyl hydrolase mbtJ [Mycobacterium tuberculosis C]
 gi|253320041|gb|ACT24644.1| acetyl hydrolase mbtJ [Mycobacterium tuberculosis KZN 1435]
 gi|339295284|gb|AEJ47395.1| esterase, putative [Mycobacterium tuberculosis CCDC5079]
 gi|339298903|gb|AEJ51013.1| esterase, putative [Mycobacterium tuberculosis CCDC5180]
Length=327

 Score =  615 bits (1586),  Expect = 2e-174, Method: Compositional matrix adjust.
 Identities = 306/306 (100%), Positives = 306/306 (100%), Gaps = 0/306 (0%)

Query  1    MVLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVY  60
            MVLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVY
Sbjct  22   MVLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVY  81

Query  61   GPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRF  120
            GPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRF
Sbjct  82   GPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRF  141

Query  121  PTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARPPAAVVLFSPL  180
            PTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARPPAAVVLFSPL
Sbjct  142  PTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARPPAAVVLFSPL  201

Query  181  IDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQV  240
            IDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQV
Sbjct  202  IDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQV  261

Query  241  GGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIRST  300
            GGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIRST
Sbjct  262  GGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIRST  321

Query  301  TARGDL  306
            TARGDL
Sbjct  322  TARGDL  327


>gi|31793562|ref|NP_856055.1| putative acetyl hydrolase MBTJ [Mycobacterium bovis AF2122/97]
 gi|57116980|ref|YP_177876.1| putative acetyl hydrolase MBTJ [Mycobacterium tuberculosis H37Rv]
 gi|121638264|ref|YP_978488.1| putative acetyl hydrolase mbtJ [Mycobacterium bovis BCG str. 
Pasteur 1173P2]
 67 more sequence titles
 Length=306

 Score =  613 bits (1581),  Expect = 9e-174, Method: Compositional matrix adjust.
 Identities = 306/306 (100%), Positives = 306/306 (100%), Gaps = 0/306 (0%)

Query  1    MVLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVY  60
            MVLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVY
Sbjct  1    MVLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVY  60

Query  61   GPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRF  120
            GPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRF
Sbjct  61   GPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRF  120

Query  121  PTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARPPAAVVLFSPL  180
            PTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARPPAAVVLFSPL
Sbjct  121  PTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARPPAAVVLFSPL  180

Query  181  IDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQV  240
            IDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQV
Sbjct  181  IDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQV  240

Query  241  GGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIRST  300
            GGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIRST
Sbjct  241  GGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIRST  300

Query  301  TARGDL  306
            TARGDL
Sbjct  301  TARGDL  306


>gi|340627396|ref|YP_004745848.1| putative acetyl hydrolase MBTJ [Mycobacterium canettii CIPT 140010059]
 gi|340005586|emb|CCC44748.1| putative acetyl hydrolase MBTJ [Mycobacterium canettii CIPT 140010059]
Length=306

 Score =  611 bits (1576),  Expect = 4e-173, Method: Compositional matrix adjust.
 Identities = 305/306 (99%), Positives = 305/306 (99%), Gaps = 0/306 (0%)

Query  1    MVLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVY  60
            MVLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVY
Sbjct  1    MVLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVY  60

Query  61   GPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRF  120
            GPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRF
Sbjct  61   GPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRF  120

Query  121  PTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARPPAAVVLFSPL  180
            PTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARPPAAVVLFSPL
Sbjct  121  PTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARPPAAVVLFSPL  180

Query  181  IDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQV  240
            IDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQV
Sbjct  181  IDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQV  240

Query  241  GGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIRST  300
            GGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAM YVAQFIRST
Sbjct  241  GGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMAYVAQFIRST  300

Query  301  TARGDL  306
            TARGDL
Sbjct  301  TARGDL  306


>gi|254365160|ref|ZP_04981206.1| acetyl hydrolase mbtJ [Mycobacterium tuberculosis str. Haarlem]
 gi|134150674|gb|EBA42719.1| acetyl hydrolase mbtJ [Mycobacterium tuberculosis str. Haarlem]
Length=306

 Score =  611 bits (1576),  Expect = 4e-173, Method: Compositional matrix adjust.
 Identities = 305/306 (99%), Positives = 305/306 (99%), Gaps = 0/306 (0%)

Query  1    MVLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVY  60
            MVLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVY
Sbjct  1    MVLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVY  60

Query  61   GPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRF  120
            GPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRF
Sbjct  61   GPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRF  120

Query  121  PTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARPPAAVVLFSPL  180
            PTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARP AAVVLFSPL
Sbjct  121  PTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARPSAAVVLFSPL  180

Query  181  IDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQV  240
            IDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQV
Sbjct  181  IDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQV  240

Query  241  GGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIRST  300
            GGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIRST
Sbjct  241  GGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIRST  300

Query  301  TARGDL  306
            TARGDL
Sbjct  301  TARGDL  306


>gi|240169930|ref|ZP_04748589.1| acetyl hydrolase MbtJ [Mycobacterium kansasii ATCC 12478]
Length=333

 Score =  434 bits (1117),  Expect = 7e-120, Method: Compositional matrix adjust.
 Identities = 223/301 (75%), Positives = 246/301 (82%), Gaps = 2/301 (0%)

Query  2    VLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVYG  61
            ++  ITGAIPP+  WG+W +R++IA L+GTFGPSLAGTRVE VNSVL DGRRV GEWV+G
Sbjct  23   IMPTITGAIPPERAWGMWLTRQLIARLLGTFGPSLAGTRVEYVNSVLADGRRVRGEWVFG  82

Query  62   PHNNAINAGPGG--GAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYR  119
            PH  A +    G  G IYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVF V+YRLAPRYR
Sbjct  83   PHMEATHRSSAGTAGVIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFCVNYRLAPRYR  142

Query  120  FPTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARPPAAVVLFSP  179
            FPTAA DVRA WDWL    GL  E +V+ ADSAGGHL+VDMLLQ +VAA PPA++VLFSP
Sbjct  143  FPTAADDVRAGWDWLPEASGLPPERIVVVADSAGGHLSVDMLLQRDVAANPPASLVLFSP  202

Query  180  LIDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQ  239
            LIDLTF L A+RE  RPDP VRA  AAR V LYY G+DP HHRL LDVAGGP LP TLIQ
Sbjct  203  LIDLTFTLSAARERLRPDPAVRAAAAARVVGLYYDGIDPTHHRLTLDVAGGPVLPNTLIQ  262

Query  240  VGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIRS  299
             G AEILEADARQL ADIRAAGG C+LQVWPDQMHVFQALPRM PEAAKAM Y  +FI +
Sbjct  263  AGAAEILEADARQLAADIRAAGGSCDLQVWPDQMHVFQALPRMAPEAAKAMAYACEFIAN  322

Query  300  T  300
            +
Sbjct  323  S  323


>gi|183983679|ref|YP_001851970.1| acetyl hydrolase MbtJ [Mycobacterium marinum M]
 gi|183177005|gb|ACC42115.1| acetyl hydrolase MbtJ [Mycobacterium marinum M]
Length=331

 Score =  425 bits (1093),  Expect = 4e-117, Method: Compositional matrix adjust.
 Identities = 223/297 (76%), Positives = 250/297 (85%), Gaps = 0/297 (0%)

Query  1    MVLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVY  60
            +++ PIT AIPP+  WGIW +R++IA LMGT GPSL+GT V+ VNSVLPDGRRVVGEWV+
Sbjct  22   IMMPPITAAIPPERAWGIWMTRQLIARLMGTLGPSLSGTHVKPVNSVLPDGRRVVGEWVH  81

Query  61   GPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRF  120
            GPH     A    GAIY+VHGSGYTMCSPRTHRRLT+WLSSLTGLPVFSVDYRLAPR+RF
Sbjct  82   GPHLPTGPAPNRSGAIYFVHGSGYTMCSPRTHRRLTAWLSSLTGLPVFSVDYRLAPRHRF  141

Query  121  PTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARPPAAVVLFSPL  180
            PTAA DVRA WDWL   CGL+ E MV+ ADSAGGHL+VD+LLQP+VAARPPA++VLFSPL
Sbjct  142  PTAADDVRAGWDWLPGACGLSPERMVVVADSAGGHLSVDLLLQPDVAARPPASLVLFSPL  201

Query  181  IDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQV  240
            IDLTF L A+RE  +PDP VRA  AAR V LY+ G+DP HHRL+LDVAGG PLP TLIQ 
Sbjct  202  IDLTFGLSAARERTKPDPAVRAATAARVVGLYHAGIDPTHHRLSLDVAGGSPLPSTLIQA  261

Query  241  GGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFI  297
            G AE+LEADARQL ADIR AGG CELQVWPDQMHVFQALPRMTPEAAKAM Y + FI
Sbjct  262  GAAEMLEADARQLAADIRTAGGRCELQVWPDQMHVFQALPRMTPEAAKAMAYASAFI  318


>gi|118618914|ref|YP_907246.1| acetyl hydrolase MbtJ [Mycobacterium ulcerans Agy99]
 gi|118571024|gb|ABL05775.1| acetyl hydrolase MbtJ [Mycobacterium ulcerans Agy99]
Length=331

 Score =  423 bits (1088),  Expect = 1e-116, Method: Compositional matrix adjust.
 Identities = 222/297 (75%), Positives = 249/297 (84%), Gaps = 0/297 (0%)

Query  1    MVLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVY  60
            +++ PI  AIPP+  WGIW +R++IA LMGT GPSL+GT V+ VNSVLPDGRRVVGEWV+
Sbjct  22   IMMPPIIAAIPPERAWGIWMTRQVIARLMGTLGPSLSGTHVKPVNSVLPDGRRVVGEWVH  81

Query  61   GPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRF  120
            GPH     A    GAIY+VHGSGYTMCSPRTHRRLT+WLSSLTGLPVFSVDYRLAPR+RF
Sbjct  82   GPHLPTGPAPNRSGAIYFVHGSGYTMCSPRTHRRLTAWLSSLTGLPVFSVDYRLAPRHRF  141

Query  121  PTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARPPAAVVLFSPL  180
            PTAA DVRA WDWL   CGL+ E MV+ ADSAGGHL+VD+LLQP+VAARPPA++VLFSPL
Sbjct  142  PTAADDVRAGWDWLPGACGLSPERMVVVADSAGGHLSVDLLLQPDVAARPPASLVLFSPL  201

Query  181  IDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQV  240
            IDLTF L A+RE  +PDP VRA  AAR V LY+ G+DP HHRL+LDVAGG PLP TLIQ 
Sbjct  202  IDLTFGLSAARERTKPDPAVRAATAARVVGLYHAGIDPTHHRLSLDVAGGSPLPSTLIQA  261

Query  241  GGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFI  297
            G AE+LEADARQL ADIR AGG CELQVWPDQMHVFQALPRMTPEAAKAM Y + FI
Sbjct  262  GAAEMLEADARQLAADIRTAGGRCELQVWPDQMHVFQALPRMTPEAAKAMAYASAFI  318


>gi|254822292|ref|ZP_05227293.1| LipK [Mycobacterium intracellulare ATCC 13950]
Length=334

 Score =  419 bits (1077),  Expect = 3e-115, Method: Compositional matrix adjust.
 Identities = 209/299 (70%), Positives = 236/299 (79%), Gaps = 5/299 (1%)

Query  2    VLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVYG  61
             LRPIT  IPP+  WG+W SR+IIA +MGTFGPSLAGTRVE V++ LPDGRRV GEWVYG
Sbjct  23   TLRPITSVIPPERAWGLWLSRQIIARIMGTFGPSLAGTRVEPVDTRLPDGRRVKGEWVYG  82

Query  62   PH---NNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRY  118
            P    +    +    GAIYYVHGSGY +CSP+THRRLTSWLSSLTGLPVF +DYRLAP++
Sbjct  83   PRTPTSETERSSTTAGAIYYVHGSGYAVCSPKTHRRLTSWLSSLTGLPVFCIDYRLAPKH  142

Query  119  RFPTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARPPAAVVLFS  178
            RFPTAA DVRA WDWL   CGL  +H+VIA DSAGGHL+VD+LLQP V    PAA+VLFS
Sbjct  143  RFPTAADDVRAGWDWLVDTCGLPPKHIVIAGDSAGGHLSVDLLLQPGV--EHPAAMVLFS  200

Query  179  PLIDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLI  238
            PL DL+F L  +RE  RPDP   A  A R V LY++GVD  H RL LDVAGGP LPPTLI
Sbjct  201  PLYDLSFELALARERIRPDPATSAANAVRLVGLYHSGVDLTHQRLTLDVAGGPALPPTLI  260

Query  239  QVGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFI  297
            Q GGAE+L+ DARQL ADIR AGG CELQ+WP Q+HVFQALPRMTPEAAKAM YVA+FI
Sbjct  261  QAGGAEMLQEDARQLAADIRTAGGRCELQIWPHQVHVFQALPRMTPEAAKAMAYVARFI  319


>gi|254774614|ref|ZP_05216130.1| LipK [Mycobacterium avium subsp. avium ATCC 25291]
Length=311

 Score =  416 bits (1069),  Expect = 2e-114, Method: Compositional matrix adjust.
 Identities = 208/298 (70%), Positives = 235/298 (79%), Gaps = 5/298 (1%)

Query  3    LRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVYGP  62
            +RPIT  IPP+  WG+W SR+IIA +MGTFGPSLAGTRVE V++ LPDGRRV GEWVYGP
Sbjct  1    MRPITSVIPPERAWGLWLSRQIIARIMGTFGPSLAGTRVELVDTRLPDGRRVKGEWVYGP  60

Query  63   H---NNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYR  119
                + A  +    GAIYYVHGSGY +CSP+THRRLTSWLSSLTGLPVF +DYRLAP++R
Sbjct  61   RTPTSEAERSSTTAGAIYYVHGSGYAVCSPKTHRRLTSWLSSLTGLPVFCIDYRLAPKHR  120

Query  120  FPTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARPPAAVVLFSP  179
            FPTAA DVRA WDWL   CGL  +H+VIA DSAGGHL+VD+LLQP V    PAA+VLFSP
Sbjct  121  FPTAADDVRAGWDWLVDTCGLLPKHIVIAGDSAGGHLSVDLLLQPGVEH--PAAMVLFSP  178

Query  180  LIDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQ  239
            L DLTF L  +RE  RPDP  RA  A R V  Y++GVD  H RL LDVA GP LPPTLIQ
Sbjct  179  LYDLTFELAKARERIRPDPATRAANAIRLVGRYHSGVDLTHERLTLDVARGPALPPTLIQ  238

Query  240  VGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFI  297
             GGAE+L+ DA QL ADIR AGG CELQ+WP Q+HVFQALPRMTPEAAKAM YVA+FI
Sbjct  239  AGGAEMLQEDAHQLAADIRTAGGRCELQIWPHQVHVFQALPRMTPEAAKAMAYVARFI  296


>gi|41408295|ref|NP_961131.1| LipK [Mycobacterium avium subsp. paratuberculosis K-10]
 gi|41396651|gb|AAS04514.1| LipK [Mycobacterium avium subsp. paratuberculosis K-10]
Length=334

 Score =  414 bits (1064),  Expect = 1e-113, Method: Compositional matrix adjust.
 Identities = 207/299 (70%), Positives = 237/299 (80%), Gaps = 5/299 (1%)

Query  2    VLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVYG  61
             LRPIT  IPP+  WG+W SR+I+A +MGTFGPSL+GTRV+ V++ LPDGRRV GEWV+G
Sbjct  23   TLRPITSVIPPERAWGLWLSRQIVARIMGTFGPSLSGTRVQSVDTRLPDGRRVKGEWVFG  82

Query  62   PHN-NAINAGPGG--GAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRY  118
            P    + ++ P     AIYY+HGSGY +CSP+THRRLTSWLSSLTGLPVF VDYRLAPR+
Sbjct  83   PRTPTSEHSRPSSIAEAIYYIHGSGYAVCSPKTHRRLTSWLSSLTGLPVFCVDYRLAPRH  142

Query  119  RFPTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARPPAAVVLFS  178
            RFPTAA DVRA WDWL + CG+  E MVIA DSAGGHL+VD+LLQP V    PAA+VLFS
Sbjct  143  RFPTAADDVRAGWDWLINTCGVLPEQMVIAGDSAGGHLSVDLLLQPGVEH--PAAMVLFS  200

Query  179  PLIDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLI  238
            PL DLTF L  +RE  RPDP  RA  A R V LY++GVD  H RL LDVA GP LPPTLI
Sbjct  201  PLYDLTFELAKARERIRPDPTTRAANAIRLVGLYHSGVDLTHERLTLDVARGPALPPTLI  260

Query  239  QVGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFI  297
            Q GGAE+L+ADA QL ADIR AGG CELQ+WP Q+HVFQALPRMTPEAAKAM YVA+FI
Sbjct  261  QAGGAEMLQADAHQLAADIRTAGGRCELQIWPHQVHVFQALPRMTPEAAKAMAYVARFI  319


>gi|336458109|gb|EGO37092.1| esterase/lipase [Mycobacterium avium subsp. paratuberculosis 
S397]
Length=305

 Score =  404 bits (1037),  Expect = 1e-110, Method: Compositional matrix adjust.
 Identities = 202/291 (70%), Positives = 232/291 (80%), Gaps = 5/291 (1%)

Query  10   IPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVYGPHN-NAIN  68
            IPP+  WG+W SR+I+A +MGTFGPSL+GTRV+ V++ LPDGRRV GEWV+GP    + +
Sbjct  2    IPPERAWGLWLSRQIVARIMGTFGPSLSGTRVQSVDTRLPDGRRVKGEWVFGPRTPTSEH  61

Query  69   AGPGG--GAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRFPTAATD  126
            + P     AIYY+HGSGY +CSP+THRRLTSWLSSLTGLPVF VDYRLAPR+RFPTAA D
Sbjct  62   SRPSSIAEAIYYIHGSGYAVCSPKTHRRLTSWLSSLTGLPVFCVDYRLAPRHRFPTAADD  121

Query  127  VRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARPPAAVVLFSPLIDLTFR  186
            VRA WDWL + CG+  E MVIA DSAGGHL+VD+LLQP V    PAA+VLFSPL DLTF 
Sbjct  122  VRAGWDWLINTCGVLPEQMVIAGDSAGGHLSVDLLLQPGVEH--PAAMVLFSPLYDLTFE  179

Query  187  LGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQVGGAEIL  246
            L  +RE  RPDP  RA  A R V LY++GVD  H RL LDVA GP LPPTLIQ GGAE+L
Sbjct  180  LAKARERIRPDPTTRAANAIRLVGLYHSGVDLTHERLTLDVARGPALPPTLIQAGGAEML  239

Query  247  EADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFI  297
            +ADA QL ADIR AGG CELQ+WP Q+HVFQALPRMTPEAAKAM YVA+FI
Sbjct  240  QADAHQLAADIRTAGGRCELQIWPHQVHVFQALPRMTPEAAKAMAYVARFI  290


>gi|118464610|ref|YP_881022.1| alpha/beta hydrolase [Mycobacterium avium 104]
 gi|118165897|gb|ABK66794.1| alpha/beta hydrolase fold domain protein [Mycobacterium avium 
104]
Length=305

 Score =  403 bits (1035),  Expect = 2e-110, Method: Compositional matrix adjust.
 Identities = 202/291 (70%), Positives = 232/291 (80%), Gaps = 5/291 (1%)

Query  10   IPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVYGPHN-NAIN  68
            IPP+  WG+W SR+I+A +MGTFGPSL+GTRV+ V++ LPDGRRV GEWV+GP    + +
Sbjct  2    IPPERAWGLWLSRQIVARIMGTFGPSLSGTRVQSVDTRLPDGRRVKGEWVFGPRTPTSEH  61

Query  69   AGPGG--GAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRFPTAATD  126
            + P     AIYY+HGSGY +CSP+THRRLTSWLSSLTGLPVF VDYRLAPR+RFPTAA D
Sbjct  62   SRPSSIAEAIYYIHGSGYAVCSPKTHRRLTSWLSSLTGLPVFCVDYRLAPRHRFPTAADD  121

Query  127  VRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARPPAAVVLFSPLIDLTFR  186
            VRA WDWL + CG+  E MVIA DSAGGHL+VD+LLQP V    PAA+VLFSPL DLTF 
Sbjct  122  VRAGWDWLINTCGVPPEQMVIAGDSAGGHLSVDLLLQPGVEH--PAAMVLFSPLYDLTFE  179

Query  187  LGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQVGGAEIL  246
            L  +RE  RPDP  RA  A R V LY++GVD  H RL LDVA GP LPPTLIQ GGAE+L
Sbjct  180  LAKARERIRPDPATRAANAIRLVGLYHSGVDLTHERLTLDVARGPVLPPTLIQAGGAEML  239

Query  247  EADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFI  297
            +ADA QL ADIR AGG CELQ+WP Q+HVFQALPRMTPEAAKAM YVA+FI
Sbjct  240  QADAHQLAADIRTAGGRCELQIWPHQVHVFQALPRMTPEAAKAMAYVARFI  290


>gi|342857781|ref|ZP_08714437.1| alpha/beta hydrolase [Mycobacterium colombiense CECT 3035]
 gi|342135114|gb|EGT88280.1| alpha/beta hydrolase [Mycobacterium colombiense CECT 3035]
Length=305

 Score =  402 bits (1034),  Expect = 3e-110, Method: Compositional matrix adjust.
 Identities = 201/291 (70%), Positives = 231/291 (80%), Gaps = 5/291 (1%)

Query  10   IPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVYGPH---NNA  66
            IPP+  WG+W SR+IIA +MGTFGPSLAGTRVE V+  LPDGRRV GEWVYGP    ++ 
Sbjct  2    IPPERAWGLWLSRQIIARIMGTFGPSLAGTRVESVDVRLPDGRRVKGEWVYGPRTPTSDT  61

Query  67   INAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRFPTAATD  126
              +    GAIYYVHGSGY +CSP+THRRLTSWLS+LTGLPVF +DYRLAP++RFPTAA D
Sbjct  62   ERSSVSEGAIYYVHGSGYAVCSPKTHRRLTSWLSALTGLPVFCIDYRLAPKHRFPTAADD  121

Query  127  VRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARPPAAVVLFSPLIDLTFR  186
            VRA WDWL +  G+  + +VIA DSAGGHL+VD+LLQPEV    PAA+VLFSPL DLTF 
Sbjct  122  VRAGWDWLVNTRGVPPKQIVIAGDSAGGHLSVDLLLQPEVEH--PAAMVLFSPLYDLTFE  179

Query  187  LGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQVGGAEIL  246
            L  +RE  RPDP  RA  A R V LY++G++  H RL LDVAG P LPPTLIQ GGAE+L
Sbjct  180  LALARERIRPDPATRAANAVRLVGLYHSGIELTHQRLTLDVAGRPALPPTLIQAGGAEML  239

Query  247  EADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFI  297
            + DARQL ADIR AGG CELQVWP Q+HVFQALPRMTPEAAKAM YVA+FI
Sbjct  240  QEDARQLAADIRTAGGRCELQVWPHQVHVFQALPRMTPEAAKAMAYVARFI  290


>gi|311743425|ref|ZP_07717231.1| alpha/beta hydrolase domain protein [Aeromicrobium marinum DSM 
15272]
 gi|311312555|gb|EFQ82466.1| alpha/beta hydrolase domain protein [Aeromicrobium marinum DSM 
15272]
Length=347

 Score =  267 bits (682),  Expect = 2e-69, Method: Compositional matrix adjust.
 Identities = 147/302 (49%), Positives = 187/302 (62%), Gaps = 11/302 (3%)

Query  2    VLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVYG  61
             LRP +  + P G  G+  +R IIA  M + G     T + ++    PDG+ V+GEWV  
Sbjct  31   TLRPFS-QVAPQGRLGVAITRAIIASSMHSLGQGAPDTDITRIRHRSPDGQ-VIGEWVRP  88

Query  62   PHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRFP  121
                  +A      I Y+HGS +  CSPRTHR+L S +++ +G P F+V YR APR+RFP
Sbjct  89   AAARRTDA-----VILYLHGSAFVACSPRTHRKLVSRIAAASGTPAFTVQYRRAPRHRFP  143

Query  122  TAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQ-PEVAARPPAAVVLFSPL  180
             A+ D   A+ WL H      + +V+A DSAGGHL VD+ L+      + P A+VL SPL
Sbjct  144  AASDDALRAYRWLVH---QGHQRIVVAGDSAGGHLAVDLALELARTGEQQPEALVLLSPL  200

Query  181  IDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQV  240
             D +F L A+REL + DP++ A RA   V  Y  GVDP HHRL L V   P LPPTLIQ 
Sbjct  201  YDTSFSLAAARELVQRDPMISAARARTLVDHYTVGVDPDHHRLRLAVREAPALPPTLIQA  260

Query  241  GGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIRST  300
            GGAE+L ADA QL ADI AAGG CELQVWP QMHVFQA+PRM PEA +A+  V +F+R  
Sbjct  261  GGAEMLAADAEQLAADITAAGGTCELQVWPGQMHVFQAMPRMAPEAGRAVGVVGRFVRRQ  320

Query  301  TA  302
             A
Sbjct  321  LA  322


>gi|333920083|ref|YP_004493664.1| alpha/beta hydrolase fold-3 domain-containing protein [Amycolicicoccus 
subflavus DQS3-9A1]
 gi|333482305|gb|AEF40865.1| Alpha/beta hydrolase fold-3 domain protein [Amycolicicoccus subflavus 
DQS3-9A1]
Length=301

 Score =  264 bits (675),  Expect = 1e-68, Method: Compositional matrix adjust.
 Identities = 148/307 (49%), Positives = 194/307 (64%), Gaps = 15/307 (4%)

Query  2    VLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVYG  61
            +LR +T  +P + P G+W +R++++  MG  GP L GTRVEQV S       V GEWV G
Sbjct  1    MLRNLTNVVPLNNP-GVWFARQLVSVSMGVLGPPLRGTRVEQVRSGA-----VRGEWVRG  54

Query  62   PHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRFP  121
            P     +      AI+Y+HGSGY +CS RTHR + S LS  +G PVF+VDYRLAP + FP
Sbjct  55   PGVTQSDR-----AIFYIHGSGYVVCSARTHRGIASRLSQASGAPVFTVDYRLAPEHPFP  109

Query  122  TAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARP-PAAVVLFSPL  180
             AA D+  A+ WL    G  ++ +V+A DSAGGHL VD+ ++      P P+A VLFSPL
Sbjct  110  AAAQDIETAYRWLLG-QGYVSDQIVVAGDSAGGHLAVDLFIENARTQTPQPSAAVLFSPL  168

Query  181  IDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHH-RLALDVAGGPPLPPTLIQ  239
            +DL+  L  ++E  R DP++ A+ A + V+LY TG  PA H RL L +A G  LPP L+Q
Sbjct  169  MDLSLSLAEAKERTRRDPMISAENARKLVSLY-TGRHPADHPRLKLGLAAGTALPPLLVQ  227

Query  240  VGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIRS  299
             GG E+L ADA  L A +  AGG CEL++WP QMHVFQALPR+ PEA  A+    QFI  
Sbjct  228  AGGEEMLSADAEYLRALVTQAGGSCELEIWPGQMHVFQALPRLVPEARPALRRAGQFITR  287

Query  300  TTARGDL  306
              +  DL
Sbjct  288  AWSSRDL  294


>gi|296141191|ref|YP_003648434.1| alpha/beta hydrolase fold-3 protein domain-containing protein 
[Tsukamurella paurometabola DSM 20162]
 gi|296029325|gb|ADG80095.1| Alpha/beta hydrolase fold-3 domain protein [Tsukamurella paurometabola 
DSM 20162]
Length=328

 Score =  258 bits (659),  Expect = 7e-67, Method: Compositional matrix adjust.
 Identities = 149/296 (51%), Positives = 186/296 (63%), Gaps = 13/296 (4%)

Query  6    ITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVYGPHNN  65
            ++G IP +   G+W +R ++A +MG  G S  GTR   V +       V G+WV GP   
Sbjct  32   VSGLIPMNQS-GVWVARGLVAAIMGALGSSPRGTRATAVRTPT-----VPGDWVLGPGVR  85

Query  66   AINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRFPTAAT  125
                  G  A+YY+HGS Y +CS RTHR L + LS++TGLPVF VDYRLAP + FP AA 
Sbjct  86   F-----GRRAVYYIHGSAYAICSARTHRSLAARLSAVTGLPVFVVDYRLAPEHPFPAAAD  140

Query  126  DVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQ-PEVAARPPAAVVLFSPLIDLT  184
            D+ A +DWL    G AA  +V+A DSAGGHL VD+++Q     A  PAAV LFSPLIDLT
Sbjct  141  DIAAGFDWLL-TQGYAAGDIVLAGDSAGGHLAVDLVVQRAREEASQPAAVALFSPLIDLT  199

Query  185  FRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQVGGAE  244
            F L A +E  R DP + A  A R V LY  G+   H RL LD+A    LPP LIQ GGAE
Sbjct  200  FGLAAEQERTRRDPAITAAAARRLVDLYTAGIPDDHPRLRLDLAFADRLPPFLIQAGGAE  259

Query  245  ILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIRST  300
            +L ADAR LD ++R  GG   L++WP  MHVFQALPR+TPEA  A+  V  F+ +T
Sbjct  260  MLSADARYLDRELRENGGTSTLEIWPGLMHVFQALPRLTPEADNALLRVRDFLAAT  315


>gi|169631131|ref|YP_001704780.1| putative lipase/esterase [Mycobacterium abscessus ATCC 19977]
 gi|169243098|emb|CAM64126.1| Putative lipase/esterase [Mycobacterium abscessus]
Length=674

 Score =  247 bits (630),  Expect = 2e-63, Method: Compositional matrix adjust.
 Identities = 152/309 (50%), Positives = 191/309 (62%), Gaps = 16/309 (5%)

Query  1    MVLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVY  60
            M LRP+   +P     G+ ASRR++A  M    P     R+++V      G R  GEWV+
Sbjct  373  MGLRPLIERVPMSR-LGVAASRRLVATAMTAGSPMPTNVRIDRVRE---QGLR--GEWVH  426

Query  61   GPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRF  120
             P ++       G  I+Y+HGSGY +CS RTHRRL + LS  +GLP FS+DYRLAP + F
Sbjct  427  APDSDR-----DGPVIFYIHGSGYVVCSSRTHRRLVARLSQASGLPAFSLDYRLAPEHPF  481

Query  121  PTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPE-VAARPPAAVVLFSP  179
            P AA DVRAA+ WL    G AA+ +VIAADSAGGHL VD++ +   +    P A+VLFSP
Sbjct  482  PAAADDVRAAYQWLLS-TGHAAKDIVIAADSAGGHLAVDLIAENHRMGVAQPGALVLFSP  540

Query  180  LIDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHH-RLALDVAGGPPLPPTLI  238
            L DLT  L A RE   PDP++ A  AAR +   YT   P    RL L +  G  LPPTLI
Sbjct  541  LADLTLGLSAERERDAPDPIISAA-AARRLVDCYTAAQPVDSPRLRLALGPGVMLPPTLI  599

Query  239  QVGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIR  298
            QVGG E++ ADA  L+  I  AGG CELQVWP Q+HVFQALPR + EA  A+   A+FI 
Sbjct  600  QVGGREMMVADAEHLELMITRAGGACELQVWPGQVHVFQALPRFSSEATAAVREAARFIT  659

Query  299  ST-TARGDL  306
            +T  AR +L
Sbjct  660  ATLAARSEL  668


>gi|333990246|ref|YP_004522860.1| acetyl hydrolase MbtJ [Mycobacterium sp. JDM601]
 gi|333486214|gb|AEF35606.1| acetyl hydrolase MbtJ [Mycobacterium sp. JDM601]
Length=291

 Score =  246 bits (627),  Expect = 5e-63, Method: Compositional matrix adjust.
 Identities = 150/282 (54%), Positives = 176/282 (63%), Gaps = 12/282 (4%)

Query  17   GIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVYGPHNNAINAGPGGGAI  76
            G+W  RR+IA +M  FG    GTR+  V      G  + GEWV GP    +  G   G  
Sbjct  5    GVWFGRRLIATIMAAFGSMPGGTRIVPVT-----GPGLRGEWVLGP---GVEFGKRAG--  54

Query  77   YYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRFPTAATDVRAAWDWLAH  136
            YYVHGS Y +CS RTHR+L +  S  TGLP+F VDYRLAP +RFP AA DV AA+ WL  
Sbjct  55   YYVHGSAYVICSARTHRKLVARWSQATGLPMFVVDYRLAPEHRFPAAADDVEAAYRWLL-  113

Query  137  VCGLAAEHMVIAADSAGGHLTVDMLL-QPEVAARPPAAVVLFSPLIDLTFRLGASRELQR  195
              G AA  +VI ADSAGGHL  DMLL   E     PAAVVLFSPLIDL+  L   +E+ R
Sbjct  114  AQGYAASDIVIGADSAGGHLACDMLLAHAEDPHFRPAAVVLFSPLIDLSLGLALQQEVVR  173

Query  196  PDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQVGGAEILEADARQLDA  255
             DP + A  A R V+LY  G D A  RL LD A    LPPTLIQVG  E+L ADAR LDA
Sbjct  174  RDPAMSARAARRMVSLYMRGHDQADPRLRLDFARADRLPPTLIQVGEYEMLSADARYLDA  233

Query  256  DIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFI  297
            ++R AG    L++WP  +HVFQALPR+ PEAA A+   A FI
Sbjct  234  ELRRAGATSTLEIWPGMVHVFQALPRLAPEAASALRRAAAFI  275


>gi|262200152|ref|YP_003271360.1| alpha/beta hydrolase [Gordonia bronchialis DSM 43247]
 gi|262083499|gb|ACY19467.1| Alpha/beta hydrolase fold-3 domain protein [Gordonia bronchialis 
DSM 43247]
Length=361

 Score =  211 bits (537),  Expect = 1e-52, Method: Compositional matrix adjust.
 Identities = 123/285 (44%), Positives = 159/285 (56%), Gaps = 4/285 (1%)

Query  18   IWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVYGPHNNAINAGPGGGAIY  77
            I A R  +   +    P  AGTR   +     DG  V GEWV G    A    P    IY
Sbjct  78   IRALRPSVNQTLVAMSPVPAGTRATGIRERF-DGGEVRGEWVTGARVAAGPIHPDQPIIY  136

Query  78   YVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRFPTAATDVRAAWDWLAHV  137
            Y+HGSGY +CS RTHR L + LS+ TG+  FS+DYR+ P YRFP A  D    + WL   
Sbjct  137  YLHGSGYVICSTRTHRGLVARLSNRTGMSAFSLDYRMGPEYRFPAAGDDAIRGYRWLLS-  195

Query  138  CGLAAEHMVIAADSAGGHLTVDMLLQPEVAARP-PAAVVLFSPLIDLTFRLGASRELQ-R  195
             G  AE +V+A DSAGGHL +D+L     A  P P A+VLFSPL D TF L    +   R
Sbjct  196  QGFRAEQIVVAGDSAGGHLALDLLSANHAAGTPQPGAMVLFSPLYDPTFSLAIEHQRSGR  255

Query  196  PDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQVGGAEILEADARQLDA  255
             DP++ A  A R + LY    DP   R+ + +     LP TLIQ GG E++ ADAR +  
Sbjct  256  RDPIIEAIAAQRILRLYTRSADPDDPRMRIRLTADMDLPDTLIQFGGLEVMGADARAIHR  315

Query  256  DIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIRST  300
            ++  +G   ELQ WPDQ HVFQ  PR TPE+ +A+   A+F+ ++
Sbjct  316  ELSNSGARVELQEWPDQGHVFQMFPRFTPESRRALAVAAEFMTAS  360


>gi|262203681|ref|YP_003274889.1| alpha/beta hydrolase [Gordonia bronchialis DSM 43247]
 gi|262087028|gb|ACY22996.1| Alpha/beta hydrolase fold-3 domain protein [Gordonia bronchialis 
DSM 43247]
Length=328

 Score =  207 bits (528),  Expect = 1e-51, Method: Compositional matrix adjust.
 Identities = 137/304 (46%), Positives = 174/304 (58%), Gaps = 18/304 (5%)

Query  2    VLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVYG  61
             LRP+   +     + +  SR ++A  M    P     RV+++N+         GEWVY 
Sbjct  20   TLRPLNEVL---SSFQVPVSRALVAAAMLVGSPPPYRVRVDRLNTGT-----FRGEWVYP  71

Query  62   PHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRFP  121
            P     +A      I YVHGSGY +CS RTHR L + LS LTG+PVFSVDYRLAP +RFP
Sbjct  72   P-----DARASRQMILYVHGSGYAICSARTHRGLVARLSRLTGMPVFSVDYRLAPEHRFP  126

Query  122  TAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQ-PEVAARPPAAVVLFSPL  180
             AA DV AA+ WL  + G  AE++ +A DSAGGHL +D+L +      R P AV L SPL
Sbjct  127  AAADDVEAAYSWLQDI-GYRAENITVAGDSAGGHLILDLLAENARHGRRQPRAVFLMSPL  185

Query  181  IDLTFRLGASRELQR--PDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLI  238
            +DL+  L   RE QR  PDP++ A  A R V  Y  G      R+A+ +     LP T+I
Sbjct  186  VDLSLGLARRRE-QRAGPDPLISARAAHRLVRNYTRGQPDDLARIAVTLDRSDDLPRTMI  244

Query  239  QVGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIR  298
              GGAE+L  DAR+    +  AG  CELQVW  QMHVFQALP + PEAA A+   A FI 
Sbjct  245  HAGGAEVLVDDAREAHRMLIEAGADCELQVWLGQMHVFQALPTLIPEAAPALAVAADFIV  304

Query  299  STTA  302
             + A
Sbjct  305  GSEA  308


>gi|296169339|ref|ZP_06850967.1| alpha/beta hydrolase domain protein [Mycobacterium parascrofulaceum 
ATCC BAA-614]
 gi|295896015|gb|EFG75703.1| alpha/beta hydrolase domain protein [Mycobacterium parascrofulaceum 
ATCC BAA-614]
Length=322

 Score =  199 bits (506),  Expect = 5e-49, Method: Compositional matrix adjust.
 Identities = 128/299 (43%), Positives = 173/299 (58%), Gaps = 12/299 (4%)

Query  2    VLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVYG  61
            +LRP+T A+P +  +G+    R++   +    P   G RVEQV++V   G RV G+W+  
Sbjct  24   ILRPLTQALPSNQ-YGLAVMDRVLRAALIASRPR-RGLRVEQVDTVFA-GDRVRGDWIVA  80

Query  62   PHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRFP  121
            P     +  P G  I Y+HG  ++MCSP+THR L   LS+ +  PVF+V YRLAPRY +P
Sbjct  81   P-----DVDPDGPPILYIHGGAFSMCSPQTHRGLLGELSAASLRPVFAVRYRLAPRYPYP  135

Query  122  TAATDVRAAWDWLAHVCGLAAEHMV-IAADSAGGHLTVDMLLQPEVAAR--PPAAVVLFS  178
             AA D   A+ WL      A+   V IA DSAGG L V   L   +A R  PP  ++L S
Sbjct  136  AAADDALRAYRWLTGSEESASNRTVAIAGDSAGGQLAVATSLG-ALAHRSPPPDGMLLMS  194

Query  179  PLIDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLI  238
            P++DLT +L  +RE +R DP   A  A R+++LY     P   R+ +  A    LPPTLI
Sbjct  195  PVVDLTCQLAMARETRRRDPFASARSATRALSLYVGDAHPTDPRVNVLAADLTGLPPTLI  254

Query  239  QVGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFI  297
            QVGG E+L  D+RQL   +RAAG    LQV+  Q+HVFQAL R+ PEA  A+     F+
Sbjct  255  QVGGREMLLDDSRQLAERMRAAGASVRLQVFRGQIHVFQALFRLLPEARHALHLSGAFL  313


>gi|343926313|ref|ZP_08765822.1| putative esterase [Gordonia alkanivorans NBRC 16433]
 gi|343763942|dbj|GAA12748.1| putative esterase [Gordonia alkanivorans NBRC 16433]
Length=353

 Score =  199 bits (505),  Expect = 6e-49, Method: Compositional matrix adjust.
 Identities = 119/271 (44%), Positives = 154/271 (57%), Gaps = 6/271 (2%)

Query  32   FGPSLAGTRVEQVNSVLPDGRRVVGEWVYGPHNNAINAGPGGGAIYYVHGSGYTMCSPRT  91
              P  +GT V  V      G+ + GEWV G    A    PG   IYY+HGSGY +CSPRT
Sbjct  78   LNPPPSGTSVVPVRETFRGGQ-IRGEWVTG--RRAAEPRPGSRVIYYLHGSGYVVCSPRT  134

Query  92   HRRLTSWLSSLTGLPVFSVDYRLAPRYRFPTAATDVRAAWDWLAHVCGLAAEHMVIAADS  151
            HR L + L +LT L  FS+DYRL P + +P A  D    + WL    G  AE +VIA DS
Sbjct  135  HRGLVARLGNLTELSAFSLDYRLGPEHPWPCAGNDAIRGYRWLL-AQGYRAEDIVIAGDS  193

Query  152  AGGHLTVDMLLQPEVAARP-PAAVVLFSPLIDLTFRLGASRELQR-PDPVVRADRAARSV  209
            AGGHL +D+L        P P ++VLFSPL D TF L    +     DP++ A  A + +
Sbjct  194  AGGHLALDILAVNHATRTPQPGSMVLFSPLYDPTFDLAVDNQRSGVHDPIINAVTARKIL  253

Query  210  ALYYTGVDPAHHRLALDVAGGPPLPPTLIQVGGAEILEADARQLDADIRAAGGICELQVW  269
             LY    DP H R+ + +  G  LP TLIQ G  E++ ADAR   A+I  AGG+  +Q W
Sbjct  254  RLYTRTADPDHPRMRIRLTPGMTLPKTLIQYGALEVMGADARATHAEIVGAGGVSVIQAW  313

Query  270  PDQMHVFQALPRMTPEAAKAMTYVAQFIRST  300
            PDQ HVFQ +PR++ EA +A+   A+FI  T
Sbjct  314  PDQGHVFQLMPRLSFEARQAVETAARFITVT  344


>gi|326383978|ref|ZP_08205662.1| alpha/beta hydrolase fold-3 domain-containing protein [Gordonia 
neofelifaecis NRRL B-59395]
 gi|326197437|gb|EGD54627.1| alpha/beta hydrolase fold-3 domain-containing protein [Gordonia 
neofelifaecis NRRL B-59395]
Length=331

 Score =  192 bits (488),  Expect = 6e-47, Method: Compositional matrix adjust.
 Identities = 110/250 (44%), Positives = 147/250 (59%), Gaps = 8/250 (3%)

Query  53   RVVGEWVYGPHNNAI-NAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVD  111
            +V GEW++      + +       +YY+HGSGY +CSPRTHR L + L+   G+  FS+D
Sbjct  78   QVRGEWIHTDGGLGVPDPASPASIVYYLHGSGYVICSPRTHRGLVARLTRRAGIGAFSLD  137

Query  112  YRLAPRYRFPTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARP-  170
            YRL P YR+P+A  D    + WL    G  AE +V+A DSAGGHL +D+L        P 
Sbjct  138  YRLGPEYRWPSAGDDAVRGYRWLL-AQGFDAEQIVVAGDSAGGHLALDLLAANHRDGVPQ  196

Query  171  PAAVVLFSPLIDLTFRLGASRELQRP---DPVVRADRAARSVALYYTGVDPAHHRLALDV  227
            P A+ LFSPL D TF+L  +R+L+     DP++    AAR + LY  G DP H R+ + +
Sbjct  197  PRAMALFSPLYDPTFQL--ARDLESRGVRDPIIDVTGAARFLGLYTAGADPDHPRMRIRL  254

Query  228  AGGPPLPPTLIQVGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAA  287
                 LPPTLIQVG  EI+  DAR +   +  AGG+ ELQ W  Q HVFQ  P  TPE+ 
Sbjct  255  TPDMDLPPTLIQVGSREIMADDARTIHRRLLDAGGLSELQEWQGQGHVFQMFPYFTPESG  314

Query  288  KAMTYVAQFI  297
            KA+   A+FI
Sbjct  315  KAVRAAAEFI  324


>gi|145225184|ref|YP_001135862.1| alpha/beta hydrolase domain-containing protein [Mycobacterium 
gilvum PYR-GCK]
 gi|145217670|gb|ABP47074.1| Alpha/beta hydrolase fold-3 domain protein [Mycobacterium gilvum 
PYR-GCK]
Length=320

 Score =  191 bits (484),  Expect = 2e-46, Method: Compositional matrix adjust.
 Identities = 121/302 (41%), Positives = 169/302 (56%), Gaps = 11/302 (3%)

Query  2    VLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVYG  61
            VLRP+T  IP +   G     R++ G +    P  A + VE+V++    G RV G+WV  
Sbjct  23   VLRPLTQVIPAND-HGFAVMDRVLRGTLVVSRPRRAIS-VEKVDTPFA-GERVRGDWV--  77

Query  62   PHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRFP  121
                A N       I Y+HG  ++MCSP THR L S LS+    PVF+V YRL PRY FP
Sbjct  78   ---KAANVASDAPPILYIHGGAFSMCSPETHRGLISELSAAARRPVFAVRYRLVPRYPFP  134

Query  122  TAATDVRAAWDWLAHVCGLAAEH--MVIAADSAGGHLTVDMLLQPEVAARP-PAAVVLFS  178
             AA D   A+ WL     + A    + +A DSAGG L     L       P P A++L S
Sbjct  135  AAADDALIAYRWLTEGSAITASSGTVALAGDSAGGQLAAATALGAREHRLPTPDAMLLMS  194

Query  179  PLIDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLI  238
            P++DLT +L   R+L+R DP   A  A+R++ LY  G DPA  R+++  A    +PPTLI
Sbjct  195  PVLDLTCQLAMDRDLRRRDPFASAISASRTIGLYVAGADPADPRISVLDADLTDMPPTLI  254

Query  239  QVGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIR  298
            QVGG E+L  D+R     ++ AG   +LQV+  Q+HVFQA+ R+ PEA +A+    +F+ 
Sbjct  255  QVGGREMLLDDSRVFAKRLQTAGVSSQLQVFRGQIHVFQAMFRLLPEAREALRLGGEFLA  314

Query  299  ST  300
            ++
Sbjct  315  TS  316


>gi|169629162|ref|YP_001702811.1| putative esterase/acetyl hydrolase [Mycobacterium abscessus ATCC 
19977]
 gi|23573432|gb|AAN38719.1| putative esterase/acetyl hydrolase [Mycobacterium abscessus]
 gi|169241129|emb|CAM62157.1| Putative esterase/acetyl hydrolase [Mycobacterium abscessus]
Length=321

 Score =  187 bits (476),  Expect = 1e-45, Method: Compositional matrix adjust.
 Identities = 118/305 (39%), Positives = 171/305 (57%), Gaps = 11/305 (3%)

Query  2    VLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVYG  61
             LRP+   +P +  +G+    R++   +    P   G  V +V++V   G  V G+W+  
Sbjct  25   TLRPLAQLMPSNA-YGLAVMDRVLRTALVASRPR-RGVTVRKVDTVFAGGP-VRGDWITT  81

Query  62   PHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRFP  121
            P   AIN  P    + Y+HG  Y+MCSP THR L   L+S +G P+F+V YRLAPRY +P
Sbjct  82   P---AIN--PHANPLLYIHGGAYSMCSPETHRGLLGELASASGRPIFAVKYRLAPRYPYP  136

Query  122  TAATDVRAAWDWLAH--VCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARP-PAAVVLFS  178
             AA D   A+ WL      G     + +A DSAGG LT+   L       P P A++L S
Sbjct  137  AAADDALNAYRWLTSGGSPGSCQRTVAVAGDSAGGQLTMATALGARAVGLPLPDAMLLMS  196

Query  179  PLIDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLI  238
            P++DL   L  +RE++R DP   A  AAR++ LY  G D    RL++  A    +PP LI
Sbjct  197  PVLDLRCELARAREIRRRDPFASAQSAARALDLYVGGADRGDPRLSVLDADLTSMPPILI  256

Query  239  QVGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIR  298
            QVGG E+L  D+R+L   +++AG   E+QV+  Q+HVFQA+ R+ PEA  A+    +F+ 
Sbjct  257  QVGGREMLIDDSRRLAERLQSAGSHVEIQVYRGQIHVFQAMFRILPEARDAIHRAGRFLE  316

Query  299  STTAR  303
            ++  R
Sbjct  317  ASEVR  321


>gi|108800159|ref|YP_640356.1| alpha/beta hydrolase fold-3 [Mycobacterium sp. MCS]
 gi|119869287|ref|YP_939239.1| alpha/beta hydrolase domain-containing protein [Mycobacterium 
sp. KMS]
 gi|126435781|ref|YP_001071472.1| alpha/beta hydrolase domain-containing protein [Mycobacterium 
sp. JLS]
 gi|108770578|gb|ABG09300.1| Alpha/beta hydrolase fold-3 [Mycobacterium sp. MCS]
 gi|119695376|gb|ABL92449.1| Alpha/beta hydrolase fold-3 domain protein [Mycobacterium sp. 
KMS]
 gi|126235581|gb|ABN98981.1| Alpha/beta hydrolase fold-3 domain protein [Mycobacterium sp. 
JLS]
Length=318

 Score =  187 bits (475),  Expect = 2e-45, Method: Compositional matrix adjust.
 Identities = 118/305 (39%), Positives = 171/305 (57%), Gaps = 11/305 (3%)

Query  2    VLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVYG  61
             LRP+   +P +  +G+    R++   +    P   G  V +V++V   G  V G+W+  
Sbjct  22   TLRPLAQLMPSNA-YGLAVMDRVLRTALVASRPR-RGVTVRKVDTVFAGGP-VRGDWITT  78

Query  62   PHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRFP  121
            P   AIN  P    + Y+HG  Y+MCSP THR L   L+S +G P+F+V YRLAPRY +P
Sbjct  79   P---AIN--PHANPLLYIHGGAYSMCSPETHRGLLGELASASGRPIFAVKYRLAPRYPYP  133

Query  122  TAATDVRAAWDWLAH--VCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARP-PAAVVLFS  178
             AA D   A+ WL      G     + +A DSAGG LT+   L       P P A++L S
Sbjct  134  AAADDALNAYRWLTSGGSPGSCQRTVAVAGDSAGGQLTMATALGARAVGLPLPDAMLLMS  193

Query  179  PLIDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLI  238
            P++DL   L  +RE++R DP   A  AAR++ LY  G D    RL++  A    +PP LI
Sbjct  194  PVLDLRCELARAREIRRRDPFASAQSAARALDLYVGGADRGDPRLSVLDADLTSMPPILI  253

Query  239  QVGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIR  298
            QVGG E+L  D+R+L   +++AG   E+QV+  Q+HVFQA+ R+ PEA  A+    +F+ 
Sbjct  254  QVGGREMLIDDSRRLAERLQSAGSHVEIQVYRGQIHVFQAMFRILPEARDAIHRAGRFLE  313

Query  299  STTAR  303
            ++  R
Sbjct  314  ASEVR  318


>gi|325001073|ref|ZP_08122185.1| putative lipase/esterase [Pseudonocardia sp. P1]
Length=319

 Score =  172 bits (437),  Expect = 5e-41, Method: Compositional matrix adjust.
 Identities = 118/299 (40%), Positives = 160/299 (54%), Gaps = 7/299 (2%)

Query  3    LRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVYGP  62
            +RP+   +P D    I ++RR++               +  V +V P G  + GEWV  P
Sbjct  8    VRPLVELVP-DTDIVIRSTRRVLRAFTDVVCRPHPAASIRPVRAVAPSGFEIRGEWVLPP  66

Query  63   HNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRFPT  122
                     G G ++YVHGSGY + S RTHR +TS L+  TG PVF+ DYRLAP +R+P 
Sbjct  67   GVGRGLRDLGRGVVFYVHGSGYAVASARTHRAITSRLAVRTGRPVFACDYRLAPTHRYPA  126

Query  123  AATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARPPAAVVLFSPLID  182
            AA DV AA+DWL    G +A   VIA DSAGG L++ +  +       PA V+LFSPL D
Sbjct  127  AADDVAAAFDWLL-AQGASAATTVIAGDSAGGQLSIALATELTARGTCPAGVLLFSPLTD  185

Query  183  LTFRLGASRELQ---RPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQ  239
            L+  L  +R+ +     DP++      R +ALY  G + A  R  +  A    L P  + 
Sbjct  186  LS--LAEARKWEDGHSRDPLISVRHLERLLALYLDGTERASARTRVTAASLTGLSPVQVH  243

Query  240  VGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIR  298
              G+E L  DAR L A +  AGG  EL  WP Q+HVFQAL    PEA +++   A F+R
Sbjct  244  ASGSEFLCGDARHLAALVERAGGDVELLEWPGQLHVFQALACFVPEATRSLREAAGFVR  302


>gi|183983135|ref|YP_001851426.1| esterase/lipase [Mycobacterium marinum M]
 gi|183176461|gb|ACC41571.1| esterase/lipase [Mycobacterium marinum M]
Length=321

 Score =  161 bits (408),  Expect = 9e-38, Method: Compositional matrix adjust.
 Identities = 114/305 (38%), Positives = 165/305 (55%), Gaps = 11/305 (3%)

Query  2    VLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVYG  61
             LRP++  IP          R +   L+G+        R          G ++ G+W+  
Sbjct  25   TLRPLSQLIPSSAHGLAVMDRVLRMALVGSRPRRSVAVRTIDTEFA---GNQIRGDWITS  81

Query  62   PHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRFP  121
            P   A++  P    + Y+HG  Y+MCSP THR L   L+S +G P+F+V YRLAPRY FP
Sbjct  82   P---AVD--PHAVPLLYIHGGAYSMCSPATHRGLLGELASASGRPIFAVRYRLAPRYPFP  136

Query  122  TAATDVRAAWDWL--AHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARP-PAAVVLFS  178
             AA D   A+ WL            + +A DSAGG LT+   L       P P +++L S
Sbjct  137  AAADDALNAYRWLVTGQPSASGERGVAVAGDSAGGQLTMATALGARSDGLPLPDSMLLMS  196

Query  179  PLIDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLI  238
            P++DLT  L  +REL+R DP   A  AAR++ LY  G D  + R+++  A    +PP LI
Sbjct  197  PVLDLTCELARARELRRRDPFASARSAARALDLYVAGADHRNERISVLDADLRSMPPILI  256

Query  239  QVGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIR  298
            QVGG E+L  D+R L   +R+AG   E+QV+  Q+HVFQA+ R+ PEA +A+     F++
Sbjct  257  QVGGREMLIDDSRHLADRLRSAGSSVEIQVYRGQIHVFQAMFRILPEAREAIHRAGNFLK  316

Query  299  STTAR  303
            ++  R
Sbjct  317  ASAHR  321


>gi|269128648|ref|YP_003302018.1| alpha/beta hydrolase fold-3 domain-containing protein [Thermomonospora 
curvata DSM 43183]
 gi|268313606|gb|ACY99980.1| Alpha/beta hydrolase fold-3 domain protein [Thermomonospora curvata 
DSM 43183]
Length=317

 Score =  159 bits (401),  Expect = 7e-37, Method: Compositional matrix adjust.
 Identities = 125/305 (41%), Positives = 154/305 (51%), Gaps = 17/305 (5%)

Query  3    LRPITGAIPPDGPWGIWASRRII--AGLMGTFGPSLAGTRVEQVNSVLPD----GRRVVG  56
            LRPI   +P   P GI  +R  +  A L+    P   G RV  V   L D    GR + G
Sbjct  10   LRPIVHRLP-GHPVGIRTARTAVEAASLLVGHHP---GARVRGVRIPLEDADGDGRHLAG  65

Query  57   EWVYGPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAP  116
            E +  P    +    G  A+ Y+HG GY +CSPR+HR +TS L+  T LPVF   YRL P
Sbjct  66   ELLV-PEKGQV----GDAAVLYLHGGGYIICSPRSHRPITSRLAVDTQLPVFVPHYRLLP  120

Query  117  RYRFPTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARP-PAAVV  175
             + FP    D  AA+ WL    G  A  +VIA DSAGGHL   +  +      P PA +V
Sbjct  121  EHPFPAPLEDALAAYRWLLEE-GYPASRIVIAGDSAGGHLAASLTGEICRTGLPTPAGLV  179

Query  176  LFSPLIDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPP  235
            L+SP +DLT  L    + Q  D  + A    R   L     +    RLAL       +PP
Sbjct  180  LYSPWVDLTCELSLEYDAQVRDAYLSAHTVRRIGRLVMGQAELNDPRLALLECSWNGVPP  239

Query  236  TLIQVGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQ  295
             LIQVGGAE L  +A +L A +  AG  C LQVW  Q HVFQ L R  PEA  AM   A+
Sbjct  240  VLIQVGGAEALRPEAERLAAALAEAGTDCRLQVWEGQFHVFQLLNRFLPEARAAMDETAR  299

Query  296  FIRST  300
            FIRS 
Sbjct  300  FIRSV  304


>gi|319948633|ref|ZP_08022757.1| Alpha/beta hydrolase fold-3 domain protein [Dietzia cinnamea 
P4]
 gi|319437714|gb|EFV92710.1| Alpha/beta hydrolase fold-3 domain protein [Dietzia cinnamea 
P4]
Length=359

 Score =  158 bits (399),  Expect = 1e-36, Method: Compositional matrix adjust.
 Identities = 92/230 (40%), Positives = 124/230 (54%), Gaps = 3/230 (1%)

Query  76   IYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRFPTAATDVRAAWDWLA  135
            IY+VHG G+T  SP +H+ L S ++S TG+PVF  DYRLAP +  P A  D  A W+WL 
Sbjct  131  IYHVHGGGFTFGSPWSHKALASRIASETGVPVFLPDYRLAPEHPHPAATEDTIAGWEWLI  190

Query  136  HVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARP-PAAVVLFSPLIDLTFRLGASRELQ  194
             + G  AE++V+A DSAGG+L + ++   E    P PA VVL SP +DL F     R+  
Sbjct  191  EM-GFPAENIVVAGDSAGGNLALQLVAHLERTRAPMPAGVVLLSPWVDLDFTDMTERDRA  249

Query  195  RPDPVVRADRAARSVALYYTGVDPAHHRLA-LDVAGGPPLPPTLIQVGGAEILEADARQL  253
            R DP +    A      Y  GVDP    ++ ++    P  PP LIQ GG EI      ++
Sbjct  250  RKDPFLALGLAELCRRQYAPGVDPDDPAISPINYEPSPDWPPFLIQAGGQEIFRGGIERM  309

Query  254  DADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIRSTTAR  303
              +    G   E Q+WPDQ HVFQ    + PEA  A+  +  F+R    R
Sbjct  310  AENFAEHGVRHEFQLWPDQFHVFQVFHPLVPEARVAVRDIGSFVRGCLRR  359


>gi|333919971|ref|YP_004493552.1| alpha/beta hydrolase domain-containing protein [Amycolicicoccus 
subflavus DQS3-9A1]
 gi|333482192|gb|AEF40752.1| Alpha/beta hydrolase domain protein [Amycolicicoccus subflavus 
DQS3-9A1]
Length=329

 Score =  151 bits (381),  Expect = 1e-34, Method: Compositional matrix adjust.
 Identities = 104/270 (39%), Positives = 148/270 (55%), Gaps = 15/270 (5%)

Query  39   TRVEQVNSVLPDGRRVVGEWVYGPHNNAINAGPGGGAIY-YVHGSGYTMCSPRTHRRLTS  97
            TR EQV     DG+ V GEWV  P      AGP  GA+  Y+HG G+ + SP +HR LTS
Sbjct  63   TRAEQVRVRFKDGQ-VRGEWVERP-----GAGPRDGAVLLYIHGGGFVVGSPLSHRGLTS  116

Query  98   WLSSLTGLPVFSVDYRLAPRYRFPTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLT  157
             LS+ T  PVFSVDYR AP + +P A  DV  A+ WL    GL+A+ +V+  DSAGGHL 
Sbjct  117  ELSARTKRPVFSVDYRRAPEHPYPAAVDDVLRAYAWLID-SGLSAQQIVVCGDSAGGHLA  175

Query  158  VDMLLQPEVAARP-PAAVVLFSPLIDLTFRLG----ASRELQRPDPVVRADRAARSVALY  212
            + +  + + A  P PA VV  SP++D T  +      S   + P     A RA   +  +
Sbjct  176  LGLPPRADRAGLPVPAGVVALSPVVDPTMAMSRRWLTSNHPKAPAYFTDAGRAL--MEPH  233

Query  213  YTGVDPAHHRLALDVAGGPPLPPTLIQVGGAEILEADARQLDADIRAAGGICELQVWPDQ  272
            + GV P+   L L       +PP L+Q   AE+L  DA     ++ AAGG  +L ++  +
Sbjct  234  WRGVAPSDPELLLTNDDLTVMPPVLVQASDAELLTGDAEHYIENLNAAGGNGKLSLYHRR  293

Query  273  MHVFQALPRMTPEAAKAMTYVAQFIRSTTA  302
            +HVFQ   R++  A++A+  +A+F+    A
Sbjct  294  IHVFQVAHRVSRTASQALDEIARFVDEVIA  323


>gi|295675448|ref|YP_003603972.1| Alpha/beta hydrolase fold-3 domain protein [Burkholderia sp. 
CCGE1002]
 gi|295435291|gb|ADG14461.1| Alpha/beta hydrolase fold-3 domain protein [Burkholderia sp. 
CCGE1002]
Length=322

 Score =  149 bits (376),  Expect = 5e-34, Method: Compositional matrix adjust.
 Identities = 97/251 (39%), Positives = 129/251 (52%), Gaps = 4/251 (1%)

Query  54   VVGEWVYGPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYR  113
            +VGEW+   H     AG     + Y HG GY  CSPRTHR LT  L++     +FS+DYR
Sbjct  60   LVGEWLERVHGG--TAGGAEATVLYCHGGGYYFCSPRTHRSLTFALATRADARLFSLDYR  117

Query  114  LAPRYRFPTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARP-PA  172
            LAP +RFP A  D  AA+  L  + G+A   +VIA DSAGG L +  L+    A  P PA
Sbjct  118  LAPEHRFPAALDDATAAYRQLLAM-GIAPSSIVIAGDSAGGGLALATLVALRDAGAPMPA  176

Query  173  AVVLFSPLIDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPP  232
              +LFSP  DL     + R     DP+       R+  +Y     P H   +   A    
Sbjct  177  GGLLFSPWTDLATTGASLRTNDSVDPMFCGASIERAAKVYLGDAPPTHPYASPVYADLRG  236

Query  233  LPPTLIQVGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTY  292
            LPP  +Q G  E+L  DAR++  +  AAG  CE +VW +  HV+       PEA +A+  
Sbjct  237  LPPLFLQAGSTEVLLDDARRVADNAHAAGVDCECEVWKNLPHVWPMFAPFMPEANRALDR  296

Query  293  VAQFIRSTTAR  303
             A F+R TT+R
Sbjct  297  AAAFVRRTTSR  307


>gi|187922642|ref|YP_001894284.1| alpha/beta hydrolase fold-3 domain-containing protein [Burkholderia 
phytofirmans PsJN]
 gi|187713836|gb|ACD15060.1| Alpha/beta hydrolase fold-3 domain protein [Burkholderia phytofirmans 
PsJN]
Length=318

 Score =  148 bits (374),  Expect = 8e-34, Method: Compositional matrix adjust.
 Identities = 101/254 (40%), Positives = 133/254 (53%), Gaps = 14/254 (5%)

Query  56   GEWVY-GPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRL  114
            GEW+      +A NAGP    + Y HG GY  CSPR+HR +   L++    PVFS+DYRL
Sbjct  62   GEWLERADRASAANAGP---TVLYCHGGGYYFCSPRSHRSIVFGLATRADGPVFSLDYRL  118

Query  115  APRYRFPTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARP-PAA  173
            AP +RFP A  D  AA+  L    G+A E +VIA DSAGG L +  L+    A  P PA 
Sbjct  119  APEHRFPAALDDATAAYRQLI-ANGIAPESIVIAGDSAGGGLALATLVALRDAGDPMPAG  177

Query  174  VVLFSPLIDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLA----LDVAG  229
             +LFSP  DL     + R     DP+       R+  +Y  G  PA H  A     D+ G
Sbjct  178  GLLFSPWTDLAATGASIRANDNLDPMFSGSAIERAGKIYL-GDTPATHPYASPIYADLHG  236

Query  230  GPPLPPTLIQVGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKA  289
               LPP  I  G  E+L  D+R++  + RAAG  CEL+VW    HV+       PEA +A
Sbjct  237  ---LPPLFIMAGSTEVLLDDSRRVADNARAAGVDCELEVWNKMPHVWPIFTPFIPEANRA  293

Query  290  MTYVAQFIRSTTAR  303
            + +   F+R  T+R
Sbjct  294  LDHAGAFVRRVTSR  307


>gi|269126738|ref|YP_003300108.1| alpha/beta hydrolase fold-3 domain-containing protein [Thermomonospora 
curvata DSM 43183]
 gi|268311696|gb|ACY98070.1| Alpha/beta hydrolase fold-3 domain protein [Thermomonospora curvata 
DSM 43183]
Length=323

 Score =  139 bits (351),  Expect = 4e-31, Method: Compositional matrix adjust.
 Identities = 111/314 (36%), Positives = 143/314 (46%), Gaps = 34/314 (10%)

Query  3    LRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLA---GTRVEQVNSVLPDGRRVVGEWV  59
            LRPI   +P    W   A R  +AGL+   G  +    G R  +V ++         EWV
Sbjct  28   LRPIIARLP----WTPLALR--MAGLLDYAGVLIVRPRGARTVKVTAL-----DCAAEWV  76

Query  60   YGPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYR  119
            YG   +          I Y HG G+  C  RTHRR+ + +S   G+P  SV YR+ P   
Sbjct  77   YGAGVSPTTRK----VILYFHGGGFVACGLRTHRRMIARISRAAGMPALSVAYRMPPNVT  132

Query  120  FPTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARP-PAAVVLFS  178
              T   D   A+ WL    G   E +V+A DSAGG+L+  M L    A  P PA +   S
Sbjct  133  IATVLEDCVNAYRWLLEQ-GYEPEDIVVAGDSAGGYLSFAMPLHAMRAGLPRPAGIAALS  191

Query  179  PLIDLTFRLGASRELQRP----DPVVRADRAARSVALYYTGV---DPAHHRLALDVAGGP  231
            P  DL       R++  P    DP + A R    V L + GV   DP    +  DV G  
Sbjct  192  PFTDLDV----DRKIAHPNAPIDPYIPAPRIRDMVRLVFPGVELTDPWLSPVHADVRG--  245

Query  232  PLPPTLIQVGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMT  291
             LPPTLIQ G  E+L  DA  +   + AAG  C LQ+W  Q HVFQ    +  E   A+ 
Sbjct  246  -LPPTLIQAGSIEVLLGDAETMARHLAAAGVPCTLQIWEGQGHVFQVFADICREGLAAIA  304

Query  292  YVAQFIRSTTARGD  305
             +  F R  TA GD
Sbjct  305  EIGAFARQVTAAGD  318


>gi|296161587|ref|ZP_06844392.1| Alpha/beta hydrolase fold-3 domain protein [Burkholderia sp. 
Ch1-1]
 gi|295888231|gb|EFG68044.1| Alpha/beta hydrolase fold-3 domain protein [Burkholderia sp. 
Ch1-1]
Length=318

 Score =  139 bits (350),  Expect = 5e-31, Method: Compositional matrix adjust.
 Identities = 101/257 (40%), Positives = 132/257 (52%), Gaps = 6/257 (2%)

Query  49   PDGRRVVGEWVYGPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVF  108
            PD   + GEW+    + A  AG G   + Y HG GY  CSPRTHR L   L++     +F
Sbjct  55   PDDAPLRGEWIER-ADGAPVAGTGP-TVLYCHGGGYYFCSPRTHRSLVFGLATRANAAIF  112

Query  109  SVDYRLAPRYRFPTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAA  168
            S+DYRLAP +RFP A  D  AA+  L    G+A E +VIA DSAGG L +  L+    A 
Sbjct  113  SLDYRLAPEHRFPAALNDTTAAYRRLIG-GGIAPESIVIAGDSAGGGLALATLVALRDAG  171

Query  169  RP-PAAVVLFSPLIDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDV  227
             P PA  +LFSP  DL     + R     DP+      A +  +Y  G  PA H  A  V
Sbjct  172  DPLPAGGLLFSPWTDLAATGASIRTNDGFDPMFSGPAIALAGKIYL-GDTPATHPYASPV  230

Query  228  -AGGPPLPPTLIQVGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEA  286
             A    LPP  I  G  E+L  D++++  + RAAG  CEL+VW    HV+       PE 
Sbjct  231  YADLHGLPPLFIMAGSTEVLLDDSQRVADNARAAGVDCELEVWKKMPHVWPIFAPFIPEG  290

Query  287  AKAMTYVAQFIRSTTAR  303
             +A+   A F+R  T+R
Sbjct  291  NRALGRAAGFVRRATSR  307


>gi|219957628|gb|ACL67845.1| lipolytic enzyme [uncultured bacterium]
Length=307

 Score =  139 bits (350),  Expect = 6e-31, Method: Compositional matrix adjust.
 Identities = 102/259 (40%), Positives = 130/259 (51%), Gaps = 12/259 (4%)

Query  50   DGRRVVGEWVYGPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFS  109
            D   V GEW+  P      A P    IYY+HG GY + S  +HR++ S LS   G    +
Sbjct  50   DAGGVPGEWIAAP-----GAAPER-VIYYLHGGGYVIGSINSHRQMVSHLSRAAGARALA  103

Query  110  VDYRLAPRYRFPTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAAR  169
            +DYRLAP   FP A  D  AA+ WL    G+    +VIA DSAGG LTV  L+    A  
Sbjct  104  IDYRLAPENPFPAAVEDATAAYRWLLS-TGVDPARVVIAGDSAGGGLTVATLVALRDAGD  162

Query  170  P-PAAVVLFSPLIDLTFRLGASRELQRP-DPVVRADRAARSVALYYTGVDPAHHRLALDV  227
            P PAA V  SP +D+   LG S   +   DP+++          Y  G DP     A   
Sbjct  163  PLPAAAVCLSPWVDME-GLGESMTTKADLDPMIQPGDILEGAKAYLGGADPRTPLAAPLY  221

Query  228  AGGPPLPPTLIQVGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAA  287
            A    LPP LI VG +E L  D+ +L    +AAG    LQVW + +HVFQ    M PE  
Sbjct  222  ADLTGLPPLLIHVGTSETLLDDSTRLAERAKAAGVNVNLQVWDEMIHVFQFFAAMLPEGQ  281

Query  288  KAMTYVAQFIRSTT--ARG  304
            +A+  + +FIR  T  ARG
Sbjct  282  QAIDRIGEFIREHTGAARG  300


>gi|223365892|pdb|3FAK|A Chain A, Structural And Functional Analysis Of A Hormone-Sensitive 
Lipase Like Este5 From A Metagenome Library
 gi|224510938|pdb|3G9T|A Chain A, Crystal Structure Of Este5, Was Soaked By P-Nitrophenyl 
Butyrate For 5sec
 gi|224510939|pdb|3G9U|A Chain A, Crystal Structure Of Este5, Was Soaked By P-Nitrophenyl 
Butyrate For 5min
 9 more sequence titles
 Length=322

 Score =  137 bits (346),  Expect = 2e-30, Method: Compositional matrix adjust.
 Identities = 87/244 (36%), Positives = 121/244 (50%), Gaps = 8/244 (3%)

Query  57   EWVYGPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAP  116
            EWV  P   A      G AI Y+HG GY M S  THR +   +S  +      +DYRLAP
Sbjct  70   EWVRAPGCQA------GKAILYLHGGGYVMGSINTHRSMVGEISRASQAAALLLDYRLAP  123

Query  117  RYRFPTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARP-PAAVV  175
             + FP A  D  AA+ WL    G   +H+ I+ DSAGG L + +L+       P PA+ +
Sbjct  124  EHPFPAAVEDGVAAYRWLLDQ-GFKPQHLSISGDSAGGGLVLAVLVSARDQGLPMPASAI  182

Query  176  LFSPLIDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPP  235
              SP  D+T    + +     DP+V      +  A Y  G D  H   + + A    LPP
Sbjct  183  PISPWADMTCTNDSFKTRAEADPMVAPGGINKMAARYLNGADAKHPYASPNFANLKGLPP  242

Query  236  TLIQVGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQ  295
             LI VG  E+L  D+ +LDA  +A G    L++W D +HV+ A   M PE  +A+  V +
Sbjct  243  LLIHVGRDEVLLDDSIKLDAKAKADGVKSTLEIWDDMIHVWHAFHPMLPEGKQAIVRVGE  302

Query  296  FIRS  299
            F+R 
Sbjct  303  FMRE  306


>gi|112434063|gb|ABI18351.1| esterase/lipase [uncultured bacterium]
Length=297

 Score =  137 bits (345),  Expect = 2e-30, Method: Compositional matrix adjust.
 Identities = 88/247 (36%), Positives = 122/247 (50%), Gaps = 8/247 (3%)

Query  57   EWVYGPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAP  116
            EWV  P   A      G AI Y+HG GY M S  THR +   +S  +      +DYRLAP
Sbjct  57   EWVRAPGCQA------GKAILYLHGGGYVMGSINTHRSMVGEISRASQAAALLLDYRLAP  110

Query  117  RYRFPTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARP-PAAVV  175
             + FP A  D  AA+ WL    G   +H+ I+ DSAGG L + +L+       P PA+ +
Sbjct  111  EHPFPAAVEDGVAAYRWLLDQ-GFKPQHLSISGDSAGGGLVLAVLVSARDQGLPMPASAI  169

Query  176  LFSPLIDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPP  235
              SP  D+T    + +     DP+V      +  A Y  G D  H   + + A    LPP
Sbjct  170  PISPWADMTCTNDSFKTRAEADPMVAPGGINKMAARYLNGADAKHPYASPNFANLKGLPP  229

Query  236  TLIQVGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQ  295
             LI VG  E+L  D+ +LDA  +A G    L++W D +HV+ A   M PE  +A+  V +
Sbjct  230  LLIHVGRDEVLLDDSIKLDAKAKADGVKSTLEIWDDMIHVWHAFHPMLPEGKQAIVRVGE  289

Query  296  FIRSTTA  302
            F+R   A
Sbjct  290  FMREQWA  296


>gi|154251800|ref|YP_001412624.1| alpha/beta hydrolase domain-containing protein [Parvibaculum 
lavamentivorans DS-1]
 gi|154155750|gb|ABS62967.1| Alpha/beta hydrolase fold-3 domain protein [Parvibaculum lavamentivorans 
DS-1]
Length=299

 Score =  137 bits (345),  Expect = 2e-30, Method: Compositional matrix adjust.
 Identities = 92/230 (40%), Positives = 130/230 (57%), Gaps = 10/230 (4%)

Query  75   AIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRFPTAATDVRAAWDWL  134
            A  Y+HG G+   SPRTHR LT  L++  G+PV++VDYRLAP + FP    D  AA+  L
Sbjct  72   AFLYIHGGGFVAGSPRTHRPLTRRLATDIGVPVYAVDYRLAPEHCFPAGLDDCVAAYRAL  131

Query  135  AHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARP-PAAVVLFSPLIDLTFRLGASREL  193
                G+AA  ++   DSAGG+LT+ + L+ +    P PAA++  SP  D+T   GASR  
Sbjct  132  LER-GIAARSILAGGDSAGGNLTLALALRLKAEGLPLPAALICLSPATDMTGS-GASRHS  189

Query  194  Q-RPDPVVRADRAARSVALYYTGV---DPAHHRLALDVAGGPPLPPTLIQVGGAEILEAD  249
                D +   +     + +Y  GV   DP    L  D+AG    PPTL QVG  E+L  D
Sbjct  190  NAEADAMFVPEMMDSLMPVYCPGVEERDPYVSPLFGDLAG---FPPTLFQVGDREMLRDD  246

Query  250  ARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIRS  299
            + ++   I+AAGG   L++WP   HV+Q    M PEA +A+T++ +F R 
Sbjct  247  SVRMADAIKAAGGEATLEIWPKVWHVWQLNADMLPEAREAITHIVRFARQ  296


>gi|167561564|ref|ZP_02354480.1| esterase, putative [Burkholderia oklahomensis EO147]
Length=321

 Score =  136 bits (343),  Expect = 3e-30, Method: Compositional matrix adjust.
 Identities = 96/249 (39%), Positives = 122/249 (49%), Gaps = 9/249 (3%)

Query  56   GEWVYGPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLA  115
            GEW+    + A    P    + Y HG GY  CSP+THR L   L+   G+  FS+DYRLA
Sbjct  63   GEWLER-ADAAPGRTPQSRMLLYFHGGGYYFCSPQTHRPLVFALTKHAGVRSFSLDYRLA  121

Query  116  PRYRFPTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARP-PAAV  174
            P + FP A  D  AA+  L    G  AE +V   DSAGG L +  L+    A  P PA  
Sbjct  122  PEHPFPAAVDDALAAYRQLV-AAGTPAESIVFGGDSAGGGLALATLVALRDAGDPLPAGA  180

Query  175  VLFSPLIDLTFRLGASRELQRPDPVVRADRAARSVALYY---TGVDPAHHRLALDVAGGP  231
            VLFSP  DL       R     DP+       R+  LY     G  P    L  D  G  
Sbjct  181  VLFSPWTDLAATGDTLRAHDGLDPMFAGAALGRAAHLYLGDTPGTHPYASPLYADFKG--  238

Query  232  PLPPTLIQVGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMT  291
             LPP  IQ G  E+L  D+R++    RAAG   EL++WP+  HV+Q      PE+A+A+ 
Sbjct  239  -LPPLFIQAGSTEVLLDDSRRVADKARAAGVHVELEIWPEMPHVWQIYVPFVPESARALE  297

Query  292  YVAQFIRST  300
              A F+R T
Sbjct  298  RAAAFVRRT  306


>gi|219957636|gb|ACL67849.1| lipolytic enzyme [uncultured bacterium]
Length=307

 Score =  136 bits (343),  Expect = 4e-30, Method: Compositional matrix adjust.
 Identities = 101/267 (38%), Positives = 131/267 (50%), Gaps = 12/267 (4%)

Query  42   EQVNSVLPDGRRVVGEWVYGPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSS  101
            E V     D   V GEW+  P      A P    IYY+HG GY + S  +HR++ S LS 
Sbjct  42   EDVKREPVDAGGVPGEWIAAP-----GAAPER-VIYYLHGGGYVIGSINSHRQMVSHLSR  95

Query  102  LTGLPVFSVDYRLAPRYRFPTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDML  161
              G    ++DYRLAP   FP A  D  AA+ WL    G+    +VIA DSAGG LTV  L
Sbjct  96   AAGARALAIDYRLAPENPFPAAVEDATAAYRWLLS-TGVDPTRVVIAGDSAGGGLTVATL  154

Query  162  LQPEVAARP-PAAVVLFSPLIDLTFRLGASRELQRP-DPVVRADRAARSVALYYTGVDPA  219
            +    A  P PAA V  SP +D+   LG S   +   DP+++          Y  G DP 
Sbjct  155  VALRDAGDPLPAAAVCLSPWVDME-GLGESMTTKADLDPMIQPGDILEGAKAYLGGADPR  213

Query  220  HHRLALDVAGGPPLPPTLIQVGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQAL  279
                A   A    LPP LI VG +E L  D+ +L    + AG    ++VW + +HVFQ  
Sbjct  214  TPLAAPLYADLTGLPPLLIHVGSSETLLDDSTRLAERAKTAGVDVSIEVWDEMIHVFQFF  273

Query  280  PRMTPEAAKAMTYVAQFIRSTT--ARG  304
              M PE  +A+  + +FIR  T  ARG
Sbjct  274  ATMLPEGQQAIDRIGEFIREHTGAARG  300


>gi|91781781|ref|YP_556987.1| putative lipase/esterase [Burkholderia xenovorans LB400]
 gi|91685735|gb|ABE28935.1| Putative lipase/esterase [Burkholderia xenovorans LB400]
Length=318

 Score =  136 bits (342),  Expect = 5e-30, Method: Compositional matrix adjust.
 Identities = 97/251 (39%), Positives = 128/251 (51%), Gaps = 8/251 (3%)

Query  56   GEWVYGPHNNAI-NAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRL  114
            GEW+     + +  AGP    + Y HG GY  CSPRTHR L   L++     +FS+DYRL
Sbjct  62   GEWIERADGSPVAGAGP---IVLYCHGGGYYFCSPRTHRSLVFGLATRANAAIFSLDYRL  118

Query  115  APRYRFPTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARP-PAA  173
            AP +RFP A  D  AA+  L    G+A E +VIA DSAGG L +  L+    A  P PA 
Sbjct  119  APEHRFPAALNDTTAAYRRLIG-GGIAPESIVIAGDSAGGGLALATLVALRDAGDPMPAG  177

Query  174  VVLFSPLIDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDV-AGGPP  232
             +LFSP  DL     +       DP+      A +  +Y  G  PA H  A  V A    
Sbjct  178  GLLFSPWTDLAATGASIHGNDGLDPMFSGPAIAPAGKIYL-GDTPATHPYASPVYADLHG  236

Query  233  LPPTLIQVGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTY  292
            LPP  I  G  E+L  D++++  + RAAG  CEL+VW    HV+       PE  +A+  
Sbjct  237  LPPLFIMAGSTEVLLDDSQRVADNARAAGVDCELEVWQKMPHVWPIFAPFIPEGNRALDR  296

Query  293  VAQFIRSTTAR  303
             A F+R  T+R
Sbjct  297  AAGFVRRATSR  307


>gi|307728425|ref|YP_003905649.1| putative lipase/esterase [Burkholderia sp. CCGE1003]
 gi|307582960|gb|ADN56358.1| putative lipase/esterase [Burkholderia sp. CCGE1003]
Length=321

 Score =  135 bits (341),  Expect = 5e-30, Method: Compositional matrix adjust.
 Identities = 103/269 (39%), Positives = 138/269 (52%), Gaps = 8/269 (2%)

Query  38   GTRVEQVNSVLPDGRRVVGEWVYGPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTS  97
            G R+ +++     G+ + GEW+        +  P    + Y HG GY  CSPRTHR +  
Sbjct  46   GWRLRELDGA--GGKSLRGEWLERTGGEPASGTPA--LVLYCHGGGYYFCSPRTHRSIVF  101

Query  98   WLSSLTGLPVFSVDYRLAPRYRFPTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLT  157
             L++    PVFS+DYRLAP +RFP A  D  AA+  L    G+    +VIA DSAGG L 
Sbjct  102  GLATRANAPVFSLDYRLAPEHRFPAALDDATAAYRQLI-AEGVPPRSIVIAGDSAGGGLA  160

Query  158  VDMLLQPEVAARP-PAAVVLFSPLIDLTFRLGASRELQRPDPVVRADRAARSVALYYTGV  216
            +  L+    A  P PA  +LFSP  DL     +  +    DP+      AR+ A  Y G 
Sbjct  161  LSTLVALRDAGDPLPAGGLLFSPWTDLAASGASIVDNDGADPMFSGPAIARA-AKVYLGD  219

Query  217  DPAHHRLALDV-AGGPPLPPTLIQVGGAEILEADARQLDADIRAAGGICELQVWPDQMHV  275
             PA H  A  V A    LPP  +  G  E+L  D+R++ A  RAAG  CEL+VW    HV
Sbjct  220  MPATHPYASPVYADLRGLPPLFMMAGSTEVLLDDSRRVAAHARAAGVDCELEVWSKMPHV  279

Query  276  FQALPRMTPEAAKAMTYVAQFIRSTTARG  304
            +       PEA +A+   A+FIR  T+RG
Sbjct  280  WPIFAPFIPEANRALDRAAEFIRHATSRG  308


>gi|167568797|ref|ZP_02361671.1| esterase, putative [Burkholderia oklahomensis C6786]
Length=321

 Score =  135 bits (339),  Expect = 1e-29, Method: Compositional matrix adjust.
 Identities = 97/249 (39%), Positives = 122/249 (49%), Gaps = 9/249 (3%)

Query  56   GEWVYGPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLA  115
            GEW+    + A    P    + Y HG GY  CSPRTHR L   L+   G+  FS+DYRLA
Sbjct  63   GEWLER-ADAAPGRTPQSRMLLYFHGGGYYFCSPRTHRPLVFALTKHAGVRSFSLDYRLA  121

Query  116  PRYRFPTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARP-PAAV  174
            P + FP A  D  AA+  L    G  AE +V   DSAGG L +  L+    A  P PA  
Sbjct  122  PEHPFPAAVDDALAAYRQLV-AAGTPAESIVFGGDSAGGGLALATLVALRDAGDPLPAGA  180

Query  175  VLFSPLIDLTFRLGASRELQRPDPVVRADRAARSVALYY---TGVDPAHHRLALDVAGGP  231
            VLFSP  DL       R     DP+       R+  LY     G  P    L  D  G  
Sbjct  181  VLFSPWTDLAATGDTLRAHDGLDPMFAGAALGRAARLYLGDTPGTHPYASPLYADFKG--  238

Query  232  PLPPTLIQVGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMT  291
             LPP  IQ G  E+L  D+R++    RAAG   EL++WP+  HV+Q      PE+A+A+ 
Sbjct  239  -LPPLFIQAGSTEVLLDDSRRVADKARAAGVHVELEIWPEMPHVWQIYVPFVPESARALE  297

Query  292  YVAQFIRST  300
              A F+R T
Sbjct  298  RAAAFVRRT  306


>gi|115352848|ref|YP_774687.1| alpha/beta hydrolase domain-containing protein [Burkholderia 
ambifaria AMMD]
 gi|115282836|gb|ABI88353.1| Alpha/beta hydrolase fold-3 domain protein [Burkholderia ambifaria 
AMMD]
Length=320

 Score =  134 bits (337),  Expect = 2e-29, Method: Compositional matrix adjust.
 Identities = 92/244 (38%), Positives = 120/244 (50%), Gaps = 4/244 (1%)

Query  56   GEWVYGPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLA  115
            GEW+     +A+     G  + Y HG GY  CS +THR L   L+   G+  FS+DYRLA
Sbjct  63   GEWL--ERTDAVATRGPGRTLLYFHGGGYYFCSTKTHRPLVFGLTKRAGVRAFSLDYRLA  120

Query  116  PRYRFPTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARP-PAAV  174
            P  RFP A  D  AA+  L  + G   E +V   DSAGG L +  L+       P PA  
Sbjct  121  PENRFPAALDDALAAYRQLLAL-GTPPESIVFGGDSAGGGLALATLVALRDRGEPLPAGA  179

Query  175  VLFSPLIDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLP  234
            +LFSP  DL    G  R    PDP+       ++  LY       H   +   A    LP
Sbjct  180  ILFSPWTDLAGTGGTMRSNDGPDPMFAGAALPKAAKLYLGDASATHPYASPFYADFTGLP  239

Query  235  PTLIQVGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVA  294
            P  IQVG  E+L  D+R++    +AAG   E++VWPD  HV+Q    M PEA  A+   A
Sbjct  240  PLFIQVGSTEVLLDDSRRVADKAKAAGVPVEIEVWPDMPHVWQLYAPMVPEARDALDRAA  299

Query  295  QFIR  298
             F+R
Sbjct  300  TFLR  303


>gi|60892969|gb|AAX37295.1| lipase/esterase [uncultured bacterium]
Length=296

 Score =  134 bits (337),  Expect = 2e-29, Method: Compositional matrix adjust.
 Identities = 106/301 (36%), Positives = 143/301 (48%), Gaps = 26/301 (8%)

Query  4    RPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVYGPH  63
            +PITG+        I  +R     + G F    A  + E+VN+       V  EWV  P 
Sbjct  16   QPITGSP------SIEETRAGFEKMAGMFSVE-ADVKSERVNA-----GGVKSEWVTAPG  63

Query  64   NNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRFPTA  123
             +A      G A+ Y+HG GY + S  THR L   +S      V  +DYRLAP + FP A
Sbjct  64   ADA------GRAVLYLHGGGYVIGSINTHRSLAGRISRAAKARVLVIDYRLAPEHPFPAA  117

Query  124  ATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARP-PAAVVLFSPLID  182
              D  AA+ W+    GL    + +A DSAGG LTV  L+    A  P PAA V  SP +D
Sbjct  118  VEDSVAAYRWMLS-TGLKPSRIAVAGDSAGGGLTVATLVAIRDAKLPVPAAGVPLSPWVD  176

Query  183  LTFRLGASRELQRPDPVVRADRAARSVALYYTGVD---PAHHRLALDVAGGPPLPPTLIQ  239
            +     + +     DP+V+ D   +  A Y  G D   P    L  D+AG   LPP LIQ
Sbjct  177  MEGLGDSMKSKAAADPMVQKDGLLQMAAAYLAGKDPRTPLAAPLYADLAG---LPPLLIQ  233

Query  240  VGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIRS  299
            VG AE L  D+ +L    R AG    L+ W + +HVFQ    +  E  +A+  +  FIR+
Sbjct  234  VGTAETLLDDSTRLAERARKAGVKVTLEPWENMIHVFQIFAPILDEGQQAIEKIGAFIRA  293

Query  300  T  300
             
Sbjct  294  N  294


>gi|170699668|ref|ZP_02890705.1| Alpha/beta hydrolase fold-3 domain protein [Burkholderia ambifaria 
IOP40-10]
 gi|170135424|gb|EDT03715.1| Alpha/beta hydrolase fold-3 domain protein [Burkholderia ambifaria 
IOP40-10]
Length=320

 Score =  134 bits (337),  Expect = 2e-29, Method: Compositional matrix adjust.
 Identities = 92/244 (38%), Positives = 121/244 (50%), Gaps = 4/244 (1%)

Query  56   GEWVYGPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLA  115
            GEW+   H +A+ A   G  + Y HG GY  CS +THR L   L+   G+  FS+DYRLA
Sbjct  63   GEWL--EHTDALAARGPGRTLLYFHGGGYYFCSTKTHRPLVFGLTKRAGVRSFSLDYRLA  120

Query  116  PRYRFPTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARP-PAAV  174
            P  RFP A  D  AA+  L  + G   E +V   DSAGG L +  L+       P PA  
Sbjct  121  PENRFPAALDDALAAYRQLLAL-GTPPESIVFGGDSAGGGLALATLVALRDRGEPLPAGA  179

Query  175  VLFSPLIDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLP  234
            +LFSP  DL       R    PDP+       ++  LY       H   +   A    LP
Sbjct  180  ILFSPWTDLAGTGDTMRSNDGPDPMFAGAALPKAAKLYLGDASATHPYASPFYADFTGLP  239

Query  235  PTLIQVGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVA  294
            P  IQVG  E+L  D+R++    +AAG   E++VWPD  HV+Q    M PE+  A+   A
Sbjct  240  PLFIQVGSTEVLLDDSRRVADKAKAAGVPVEIEVWPDMPHVWQLYAPMVPESRDALDRAA  299

Query  295  QFIR  298
             F+R
Sbjct  300  AFLR  303


>gi|171319380|ref|ZP_02908489.1| Alpha/beta hydrolase fold-3 domain protein [Burkholderia ambifaria 
MEX-5]
 gi|171095416|gb|EDT40389.1| Alpha/beta hydrolase fold-3 domain protein [Burkholderia ambifaria 
MEX-5]
Length=320

 Score =  134 bits (336),  Expect = 2e-29, Method: Compositional matrix adjust.
 Identities = 92/247 (38%), Positives = 121/247 (49%), Gaps = 4/247 (1%)

Query  56   GEWVYGPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLA  115
            GEW+     +A+ A   G  + Y HG GY  CS +THR L   L+   G+  FS+DYRLA
Sbjct  63   GEWL--ERTDAVAARGPGRTLLYFHGGGYYFCSTKTHRPLVFGLTKRAGVRSFSLDYRLA  120

Query  116  PRYRFPTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARP-PAAV  174
            P  RFP A  D  AA+  L  + G   E +V   DSAGG L +  L+       P PA  
Sbjct  121  PENRFPAALDDALAAYRQLLAL-GTPPESIVFGGDSAGGGLALATLVALRDRGEPLPAGA  179

Query  175  VLFSPLIDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLP  234
            +LFSP  DL    G  R    PDP+       ++  LY       H   +   A    LP
Sbjct  180  ILFSPWTDLAGTGGTMRSNDGPDPMFAGAALPKAAKLYLGDASATHPYASPFYADFTGLP  239

Query  235  PTLIQVGGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVA  294
            P  IQ G  E+L  D+R++    +AAG   E++VWPD  HV+Q    M PE+  A+   A
Sbjct  240  PLFIQAGSTEVLLDDSRRVADKAKAAGVPVEIEVWPDMPHVWQLYAPMVPESRDALDRAA  299

Query  295  QFIRSTT  301
             F+R  T
Sbjct  300  AFLRRVT  306


>gi|45775276|gb|AAS77236.1| lipase/esterase [uncultured bacterium]
Length=296

 Score =  132 bits (333),  Expect = 6e-29, Method: Compositional matrix adjust.
 Identities = 101/283 (36%), Positives = 135/283 (48%), Gaps = 26/283 (9%)

Query  34   PSLAGTRV--EQVNSVLP----------DGRRVVGEWVYGPHNNAINAGPGGGAIYYVHG  81
            PS+A TR   EQ+ ++ P          +   V  EWV  P  +A      G A+ Y+HG
Sbjct  22   PSIAETRAGFEQMAAMFPVEADVKSEPVNAGGVKSEWVTAPGADA------GRAVLYLHG  75

Query  82   SGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRFPTAATDVRAAWDWLAHVCGLA  141
             GY + S  THR L   +S      V  +DYRLAP + FP A  D  AA+ W+    GL 
Sbjct  76   GGYVIGSINTHRSLAGRISRAAKARVLVIDYRLAPEHPFPAAVEDSVAAYRWMLS-TGLK  134

Query  142  AEHMVIAADSAGGHLTVDMLLQPEVAARP-PAAVVLFSPLIDLTFRLGASRELQRPDPVV  200
               + +A DSAGG LTV  L+    A  P PAA V  SP +D+     + +     DP+V
Sbjct  135  PSRIAVAGDSAGGGLTVATLVAIRDAKLPLPAAGVPLSPWVDMEGVGDSMKSKAAADPMV  194

Query  201  RADRAARSVALYYTGVD---PAHHRLALDVAGGPPLPPTLIQVGGAEILEADARQLDADI  257
            + D        Y  G D   P    L  D+AG   LPP LIQVG AE L  D+ +L    
Sbjct  195  QKDGLIEMAKAYLGGKDPRTPLAAPLYADLAG---LPPLLIQVGTAETLLDDSTRLAERA  251

Query  258  RAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIRST  300
            R AG    L+ W + +HVFQ    +  E  +A+  +  FIR+ 
Sbjct  252  RKAGVKVTLEPWENMVHVFQIFAPILDEGQQAIDKIGAFIRAN  294


>gi|154251326|ref|YP_001412150.1| alpha/beta hydrolase domain-containing protein [Parvibaculum 
lavamentivorans DS-1]
 gi|154155276|gb|ABS62493.1| Alpha/beta hydrolase fold-3 domain protein [Parvibaculum lavamentivorans 
DS-1]
Length=310

 Score =  132 bits (332),  Expect = 7e-29, Method: Compositional matrix adjust.
 Identities = 86/226 (39%), Positives = 116/226 (52%), Gaps = 2/226 (0%)

Query  73   GGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRFPTAATDVRAAWD  132
            G  + Y HG GY + S  +HR +TS L++ +   V SVDY LAP + FP A  D   A+ 
Sbjct  71   GPVVLYFHGGGYLVGSSVSHRHVTSRLAAASKGRVLSVDYALAPEHPFPAAVNDGLKAYR  130

Query  133  WLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARP-PAAVVLFSPLIDLTFRLGASR  191
            WL    G A E + I  DSAGG LTV  LL    A  P PA+ VL SP  DLT    +  
Sbjct  131  WLLDK-GHAPERISIGGDSAGGGLTVATLLAAREAGLPMPASAVLISPWTDLTCATESYN  189

Query  192  ELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQVGGAEILEADAR  251
                 DP+++      + A Y  G DP     + + A    LPP LI VG  E+L  D+R
Sbjct  190  SCADLDPMIQGAGIRETAAAYLNGADPRDPLASPNFADLTGLPPMLIHVGTDEVLLDDSR  249

Query  252  QLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFI  297
             L    +AAG   EL+VW   +HV+ A  +M PE  +A+  +  ++
Sbjct  250  DLAKRAKAAGVEAELEVWEGMIHVWHAFYQMLPEGEQALDRLGAYL  295



Lambda     K      H
   0.322    0.138    0.437 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Effective search space used: 518193349062


  Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
    Posted date:  Sep 5, 2011  4:36 AM
  Number of letters in database: 5,219,829,388
  Number of sequences in database:  15,229,318



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40