BLASTP 2.2.25+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 17,799,605 sequences; 6,109,862,990 total letters Query= Rv2395A Length=71 Score E Sequences producing significant alignments: (Bits) Value gi|15841910|ref|NP_336947.1| hypothetical protein MT2466 [Mycoba... 136 1e-30 gi|167968724|ref|ZP_02551001.1| hypothetical protein MtubH3_1206... 124 5e-27 gi|255261649|ref|ZP_05340991.1| pyruvate carboxylase [Thalassiob... 35.0 3.8 gi|379754602|ref|YP_005343274.1| unnamed protein product [Mycoba... 34.7 5.7 >gi|15841910|ref|NP_336947.1| hypothetical protein MT2466 [Mycobacterium tuberculosis CDC1551] gi|254232533|ref|ZP_04925860.1| hypothetical protein TBCG_02339 [Mycobacterium tuberculosis C] gi|289570542|ref|ZP_06450769.1| conserved hypothetical protein [Mycobacterium tuberculosis T17] gi|13882180|gb|AAK46761.1| hypothetical protein MT2466 [Mycobacterium tuberculosis CDC1551] gi|124601592|gb|EAY60602.1| hypothetical protein TBCG_02339 [Mycobacterium tuberculosis C] gi|289544296|gb|EFD47944.1| conserved hypothetical protein [Mycobacterium tuberculosis T17] gi|358232583|dbj|GAA46075.1| hypothetical protein NCGM2209_2706 [Mycobacterium tuberculosis NCGM2209] Length=71 Score = 136 bits (343), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 70/71 (99%), Positives = 71/71 (100%), Gaps = 0/71 (0%) Query 1 LTMTASVAKVTAARPEPSAAWAEARRRVRQRREDMLRHPAFLSKQLPAEPADDDGVAAVY 60 +TMTASVAKVTAARPEPSAAWAEARRRVRQRREDMLRHPAFLSKQLPAEPADDDGVAAVY Sbjct 1 MTMTASVAKVTAARPEPSAAWAEARRRVRQRREDMLRHPAFLSKQLPAEPADDDGVAAVY 60 Query 61 DIAIARRRRPA 71 DIAIARRRRPA Sbjct 61 DIAIARRRRPA 71 >gi|167968724|ref|ZP_02551001.1| hypothetical protein MtubH3_12065 [Mycobacterium tuberculosis H37Ra] gi|294994497|ref|ZP_06800188.1| hypothetical protein Mtub2_08273 [Mycobacterium tuberculosis 210] gi|379028688|dbj|BAL66421.1| hypothetical protein ERDMAN_2632 [Mycobacterium tuberculosis str. Erdman = ATCC 35801] Length=65 Score = 124 bits (311), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 64/65 (99%), Positives = 65/65 (100%), Gaps = 0/65 (0%) Query 7 VAKVTAARPEPSAAWAEARRRVRQRREDMLRHPAFLSKQLPAEPADDDGVAAVYDIAIAR 66 +AKVTAARPEPSAAWAEARRRVRQRREDMLRHPAFLSKQLPAEPADDDGVAAVYDIAIAR Sbjct 1 MAKVTAARPEPSAAWAEARRRVRQRREDMLRHPAFLSKQLPAEPADDDGVAAVYDIAIAR 60 Query 67 RRRPA 71 RRRPA Sbjct 61 RRRPA 65 >gi|255261649|ref|ZP_05340991.1| pyruvate carboxylase [Thalassiobium sp. R2A62] gi|255103984|gb|EET46658.1| pyruvate carboxylase [Thalassiobium sp. R2A62] Length=1147 Score = 35.0 bits (79), Expect = 3.8, Method: Compositional matrix adjust. Identities = 25/74 (34%), Positives = 37/74 (50%), Gaps = 16/74 (21%) Query 7 VAKVTAARPEPSAAWAEARRRVRQRR-----------EDMLRHPAFLSKQLPAEPADDDG 55 + KVTA P P AA A R +R+ R E++L+HP FLS + + DD Sbjct 396 LEKVTAWAPTPEAAIARMDRALREFRIRGVSTNIAFVENLLKHPTFLSNEYTTKFIDD-- 453 Query 56 VAAVYDIAIARRRR 69 A++D ++RR Sbjct 454 TPALFDF---KKRR 464 >gi|379754602|ref|YP_005343274.1| unnamed protein product [Mycobacterium intracellulare MOTT-02] gi|378804818|gb|AFC48953.1| hypothetical protein OCO_25900 [Mycobacterium intracellulare MOTT-02] Length=71 Score = 34.7 bits (78), Expect = 5.7, Method: Compositional matrix adjust. Identities = 17/45 (38%), Positives = 27/45 (60%), Gaps = 0/45 (0%) Query 1 LTMTASVAKVTAARPEPSAAWAEARRRVRQRREDMLRHPAFLSKQ 45 +T+ A+ + T + WA A+RR R+R E M RHP+F ++Q Sbjct 1 MTIVANTQEGTVIFLQSHPVWAAAQRRERERSEAMRRHPSFRTRQ 45 Lambda K H 0.320 0.128 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Effective search space used: 149645439300 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects Posted date: Apr 10, 2012 4:41 PM Number of letters in database: 6,109,862,990 Number of sequences in database: 17,799,605 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 11 Window for multiple hits: 40