BLASTP 2.2.25+
Reference:
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database
search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
15,229,318 sequences; 5,219,829,388 total letters
Query= Rv2665
Length=93
Score E
Sequences producing significant alignments: (Bits) Value
gi|15609802|ref|NP_217181.1| hypothetical protein Rv2665 [Mycoba... 181 3e-44
gi|315441603|ref|YP_004074480.1| RNA polymerase sigma factor, si... 78.2 4e-13
gi|258655053|ref|YP_003204209.1| hypothetical protein Namu_4947 ... 43.5 0.009
gi|336117532|ref|YP_004572300.1| hypothetical protein MLP_18830 ... 43.1 0.013
gi|258651520|ref|YP_003200676.1| hypothetical protein Namu_1284 ... 43.1 0.013
gi|111026944|ref|YP_708922.1| hypothetical protein RHA1_ro11117 ... 43.1 0.014
gi|120401572|ref|YP_951401.1| hypothetical protein Mvan_0551 [My... 42.7 0.019
gi|296169419|ref|ZP_06851041.1| conserved hypothetical protein [... 40.4 0.079
gi|289444360|ref|ZP_06434104.1| conserved hypothetical protein [... 38.9 0.23
gi|308232250|ref|ZP_07415430.2| hypothetical protein TMAG_03193 ... 38.9 0.24
gi|15609948|ref|NP_217327.1| hypothetical protein Rv2811 [Mycoba... 38.9 0.26
gi|289448470|ref|ZP_06438214.1| conserved hypothetical protein [... 38.5 0.31
gi|289575509|ref|ZP_06455736.1| conserved hypothetical protein [... 36.2 1.4
gi|226349348|ref|YP_002776462.1| hypothetical protein ROP_pROB01... 36.2 1.9
>gi|15609802|ref|NP_217181.1| hypothetical protein Rv2665 [Mycobacterium tuberculosis H37Rv]
gi|15842204|ref|NP_337241.1| hypothetical protein MT2739 [Mycobacterium tuberculosis CDC1551]
gi|31793837|ref|NP_856330.1| hypothetical protein Mb2684 [Mycobacterium bovis AF2122/97]
42 more sequence titles
Length=93
Score = 181 bits (459), Expect = 3e-44, Method: Compositional matrix adjust.
Identities = 93/93 (100%), Positives = 93/93 (100%), Gaps = 0/93 (0%)
Query 1 MIVVRTAEAAEQALTEGQLVCPRRGCGDTLRRWRYGRRRHVRSLGSQVIDVRPQRVRCRR 60
MIVVRTAEAAEQALTEGQLVCPRRGCGDTLRRWRYGRRRHVRSLGSQVIDVRPQRVRCRR
Sbjct 1 MIVVRTAEAAEQALTEGQLVCPRRGCGDTLRRWRYGRRRHVRSLGSQVIDVRPQRVRCRR 60
Query 61 CESTHVLLPAALQPRLGRGGGGQLRPGVWCTGR 93
CESTHVLLPAALQPRLGRGGGGQLRPGVWCTGR
Sbjct 61 CESTHVLLPAALQPRLGRGGGGQLRPGVWCTGR 93
>gi|315441603|ref|YP_004074480.1| RNA polymerase sigma factor, sigma-70 family [Mycobacterium sp.
Spyr1]
gi|315265258|gb|ADU01999.1| RNA polymerase sigma factor, sigma-70 family [Mycobacterium sp.
Spyr1]
Length=192
Score = 78.2 bits (191), Expect = 4e-13, Method: Compositional matrix adjust.
Identities = 38/75 (51%), Positives = 50/75 (67%), Gaps = 2/75 (2%)
Query 1 MIVVRTAEAAEQALTEGQLVCPRRGCGDTLRRWRYGRRRHVRSLGSQVIDVRPQRVRCRR 60
MIV+R + AE L + + CP CG TL +W Y R R VR+ G+ + VRP+R+RCR
Sbjct 1 MIVIRRPQDAEAHLADAVMCCPH--CGGTLAKWGYARERTVRAPGAATVTVRPRRLRCRN 58
Query 61 CESTHVLLPAALQPR 75
C +TH+LLP ALQPR
Sbjct 59 CTTTHILLPTALQPR 73
>gi|258655053|ref|YP_003204209.1| hypothetical protein Namu_4947 [Nakamurella multipartita DSM
44233]
gi|258558278|gb|ACV81220.1| hypothetical protein Namu_4947 [Nakamurella multipartita DSM
44233]
Length=197
Score = 43.5 bits (101), Expect = 0.009, Method: Compositional matrix adjust.
Identities = 26/62 (42%), Positives = 34/62 (55%), Gaps = 6/62 (9%)
Query 11 EQALTEGQLVCPRRGCGDTLRRWRYGRRRHVRSLGSQVIDVRPQRVRCRRCESTHVLLPA 70
E LT G + CP C L W + RRR VR +G+ ++P+R RC C THVLLP
Sbjct 12 ESRLTAGGVPCPV--CPGVLTPWGWARRREVRGVGT----LQPRRARCSLCLVTHVLLPV 65
Query 71 AL 72
+
Sbjct 66 TV 67
>gi|336117532|ref|YP_004572300.1| hypothetical protein MLP_18830 [Microlunatus phosphovorus NM-1]
gi|334685312|dbj|BAK34897.1| hypothetical protein MLP_18830 [Microlunatus phosphovorus NM-1]
Length=205
Score = 43.1 bits (100), Expect = 0.013, Method: Compositional matrix adjust.
Identities = 30/73 (42%), Positives = 37/73 (51%), Gaps = 6/73 (8%)
Query 1 MIVVRTAEA-AEQALTEGQLVCPRRGCGDTLRRWRYGRRRHVRSLGSQVIDVRPQRVRCR 59
M+ V A E L G+L CP GC +LR W + R R V L + RP+R RC
Sbjct 1 MLTVEADRAQVESRLAGGRLACP--GCAASLRPWGWARPRGVWGLPGLL---RPRRARCP 55
Query 60 RCESTHVLLPAAL 72
C THVLLP +
Sbjct 56 GCGVTHVLLPVTV 68
>gi|258651520|ref|YP_003200676.1| hypothetical protein Namu_1284 [Nakamurella multipartita DSM
44233]
gi|258652264|ref|YP_003201420.1| hypothetical protein Namu_2051 [Nakamurella multipartita DSM
44233]
gi|258653877|ref|YP_003203033.1| hypothetical protein Namu_3745 [Nakamurella multipartita DSM
44233]
gi|258654633|ref|YP_003203789.1| hypothetical protein Namu_4521 [Nakamurella multipartita DSM
44233]
gi|258554745|gb|ACV77687.1| hypothetical protein Namu_1284 [Nakamurella multipartita DSM
44233]
gi|258555489|gb|ACV78431.1| hypothetical protein Namu_2051 [Nakamurella multipartita DSM
44233]
gi|258557102|gb|ACV80044.1| hypothetical protein Namu_3745 [Nakamurella multipartita DSM
44233]
gi|258557858|gb|ACV80800.1| hypothetical protein Namu_4521 [Nakamurella multipartita DSM
44233]
Length=197
Score = 43.1 bits (100), Expect = 0.013, Method: Compositional matrix adjust.
Identities = 27/65 (42%), Positives = 34/65 (53%), Gaps = 6/65 (9%)
Query 8 EAAEQALTEGQLVCPRRGCGDTLRRWRYGRRRHVRSLGSQVIDVRPQRVRCRRCESTHVL 67
+ E LT G + CP C L W + RRR VR +G +RP+R RC C THVL
Sbjct 9 DLVESRLTGGGVPCPV--CPGVLAPWGWARRRDVRGVGL----LRPRRARCSSCLITHVL 62
Query 68 LPAAL 72
LP +
Sbjct 63 LPVTV 67
>gi|111026944|ref|YP_708922.1| hypothetical protein RHA1_ro11117 [Rhodococcus jostii RHA1]
gi|110825483|gb|ABH00764.1| conserved hypothetical protein [Rhodococcus jostii RHA1]
Length=218
Score = 43.1 bits (100), Expect = 0.014, Method: Compositional matrix adjust.
Identities = 26/62 (42%), Positives = 32/62 (52%), Gaps = 4/62 (6%)
Query 11 EQALTEGQLVCPRRGCGDTLRRWRYGRRRHVRSLGSQVIDVRPQRVRCRRCESTHVLLPA 70
E L+ G + CP G L W + R R V + + V RP+R RCR C THVLLP
Sbjct 12 ESRLSRGDMSCPS-CTGGVLAGWGFARPRPVAGMAAPV---RPRRARCRGCAVTHVLLPV 67
Query 71 AL 72
L
Sbjct 68 TL 69
>gi|120401572|ref|YP_951401.1| hypothetical protein Mvan_0551 [Mycobacterium vanbaalenii PYR-1]
gi|120401588|ref|YP_951417.1| hypothetical protein Mvan_0570 [Mycobacterium vanbaalenii PYR-1]
gi|120402692|ref|YP_952521.1| hypothetical protein Mvan_1687 [Mycobacterium vanbaalenii PYR-1]
7 more sequence titles
Length=199
Score = 42.7 bits (99), Expect = 0.019, Method: Compositional matrix adjust.
Identities = 28/69 (41%), Positives = 33/69 (48%), Gaps = 4/69 (5%)
Query 11 EQALTEGQLVCPRRGCGDTLRRWRYGRRRHVRSLGSQVIDVRPQRVRCRRCESTHVLLPA 70
E L+ G + CP G L W + R R V L VRP+R RCR C THVLLP
Sbjct 12 ESRLSGGAIACPS-CVGGVLGGWGFARSRQVEGLDH---PVRPRRARCRSCLVTHVLLPV 67
Query 71 ALQPRLGRG 79
+ R G
Sbjct 68 TVLLRRAHG 76
>gi|296169419|ref|ZP_06851041.1| conserved hypothetical protein [Mycobacterium parascrofulaceum
ATCC BAA-614]
gi|295895921|gb|EFG75614.1| conserved hypothetical protein [Mycobacterium parascrofulaceum
ATCC BAA-614]
Length=192
Score = 40.4 bits (93), Expect = 0.079, Method: Compositional matrix adjust.
Identities = 25/62 (41%), Positives = 32/62 (52%), Gaps = 3/62 (4%)
Query 8 EAAEQALTEGQLVCPRRGCGDTLRRWRYGRRRHVRSLGSQVIDVRPQRVRCRRCESTHVL 67
+ E+ L G+L CP C L RW + R R +R V + P+R RC C THVL
Sbjct 37 DVVERRLAAGELSCP--ACSSVLARWGWARPRQLRGRDGSV-RLCPRRSRCTGCGVTHVL 93
Query 68 LP 69
LP
Sbjct 94 LP 95
>gi|289444360|ref|ZP_06434104.1| conserved hypothetical protein [Mycobacterium tuberculosis T46]
gi|289417279|gb|EFD14519.1| conserved hypothetical protein [Mycobacterium tuberculosis T46]
Length=175
Score = 38.9 bits (89), Expect = 0.23, Method: Compositional matrix adjust.
Identities = 27/70 (39%), Positives = 35/70 (50%), Gaps = 4/70 (5%)
Query 1 MIVVRT-AEAAEQALTEGQLVCPRRGCGDTLRRWRYGRRRHVRSLGSQVIDVRPQRVRCR 59
M+ V + E+ L G+L CP CG L W R R +R V ++ P+R RC
Sbjct 1 MVTVEADVDQVERRLAAGELSCP--SCGGVLAGWGRARSRQLRGPAGPV-ELCPRRSRCT 57
Query 60 RCESTHVLLP 69
C THVLLP
Sbjct 58 GCGVTHVLLP 67
>gi|308232250|ref|ZP_07415430.2| hypothetical protein TMAG_03193 [Mycobacterium tuberculosis SUMu001]
gi|308369866|ref|ZP_07419331.2| hypothetical protein TMBG_02945 [Mycobacterium tuberculosis SUMu002]
gi|308371136|ref|ZP_07423946.2| hypothetical protein TMCG_02057 [Mycobacterium tuberculosis SUMu003]
21 more sequence titles
Length=215
Score = 38.9 bits (89), Expect = 0.24, Method: Compositional matrix adjust.
Identities = 25/61 (41%), Positives = 32/61 (53%), Gaps = 3/61 (4%)
Query 11 EQALTEGQLVCPRRGCGDTLRRWRYGRRRHVRSLGSQVIDVRPQRVRCRRCESTHVLLPA 70
E+ L G+L CP CG L W R R +R V ++ P+R RC C THVLLP
Sbjct 25 ERRLAAGELSCP--SCGGVLAGWGRARSRQLRGPAGPV-ELCPRRSRCTGCGVTHVLLPV 81
Query 71 A 71
+
Sbjct 82 S 82
>gi|15609948|ref|NP_217327.1| hypothetical protein Rv2811 [Mycobacterium tuberculosis H37Rv]
gi|31793986|ref|NP_856479.1| hypothetical protein Mb2834 [Mycobacterium bovis AF2122/97]
gi|121638690|ref|YP_978914.1| hypothetical protein BCG_2829 [Mycobacterium bovis BCG str. Pasteur
1173P2]
41 more sequence titles
Length=202
Score = 38.9 bits (89), Expect = 0.26, Method: Compositional matrix adjust.
Identities = 27/72 (38%), Positives = 36/72 (50%), Gaps = 4/72 (5%)
Query 1 MIVVRT-AEAAEQALTEGQLVCPRRGCGDTLRRWRYGRRRHVRSLGSQVIDVRPQRVRCR 59
M+ V + E+ L G+L CP CG L W R R +R V ++ P+R RC
Sbjct 1 MVTVEADVDQVERRLAAGELSCP--SCGGVLAGWGRARSRQLRGPAGPV-ELCPRRSRCT 57
Query 60 RCESTHVLLPAA 71
C THVLLP +
Sbjct 58 GCGVTHVLLPVS 69
>gi|289448470|ref|ZP_06438214.1| conserved hypothetical protein [Mycobacterium tuberculosis CPHL_A]
gi|289421428|gb|EFD18629.1| conserved hypothetical protein [Mycobacterium tuberculosis CPHL_A]
Length=202
Score = 38.5 bits (88), Expect = 0.31, Method: Compositional matrix adjust.
Identities = 27/72 (38%), Positives = 36/72 (50%), Gaps = 4/72 (5%)
Query 1 MIVVRT-AEAAEQALTEGQLVCPRRGCGDTLRRWRYGRRRHVRSLGSQVIDVRPQRVRCR 59
M+ V + E+ L G+L CP CG L W R R +R V ++ P+R RC
Sbjct 1 MVTVEADVDQVERRLAAGELSCP--SCGGVLAGWGRARSRKLRGPAGPV-ELCPRRSRCT 57
Query 60 RCESTHVLLPAA 71
C THVLLP +
Sbjct 58 GCGVTHVLLPVS 69
>gi|289575509|ref|ZP_06455736.1| conserved hypothetical protein [Mycobacterium tuberculosis K85]
gi|289539940|gb|EFD44518.1| conserved hypothetical protein [Mycobacterium tuberculosis K85]
Length=202
Score = 36.2 bits (82), Expect = 1.4, Method: Compositional matrix adjust.
Identities = 26/72 (37%), Positives = 35/72 (49%), Gaps = 4/72 (5%)
Query 1 MIVVRT-AEAAEQALTEGQLVCPRRGCGDTLRRWRYGRRRHVRSLGSQVIDVRPQRVRCR 59
M+ V + E+ L G+L CP CG L W R +R V ++ P+R RC
Sbjct 1 MVTVEADVDQVERRLAAGELSCP--SCGGVLAGWGRAGSRQLRGPAGPV-ELCPRRSRCT 57
Query 60 RCESTHVLLPAA 71
C THVLLP +
Sbjct 58 GCGVTHVLLPVS 69
>gi|226349348|ref|YP_002776462.1| hypothetical protein ROP_pROB01-01110 [Rhodococcus opacus B4]
gi|226245263|dbj|BAH55610.1| hypothetical protein [Rhodococcus opacus B4]
Length=195
Score = 36.2 bits (82), Expect = 1.9, Method: Compositional matrix adjust.
Identities = 17/26 (66%), Positives = 19/26 (74%), Gaps = 0/26 (0%)
Query 50 DVRPQRVRCRRCESTHVLLPAALQPR 75
D P RVRCR C +TH+LLP ALQ R
Sbjct 51 DRAPTRVRCRDCGATHILLPTALQVR 76
Lambda K H
0.327 0.141 0.468
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 127811470620
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Sep 5, 2011 4:36 AM
Number of letters in database: 5,219,829,388
Number of sequences in database: 15,229,318
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40