BLAST Search Results

BLASTP 2.2.25+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,229,318 sequences; 5,219,829,388 total letters Query= Rv3024c Length=367 Score E Sequences producing significant alignments: (Bits) Value gi|15610161|ref|NP_217540.1| tRNA-specific 2-thiouridylase MnmA ... 732 0.0 gi|339633032|ref|YP_004724674.1| tRNA (5-methylaminomethyl-2-thi... 730 0.0 gi|167970222|ref|ZP_02552499.1| tRNA (5-methylaminomethyl-2-thio... 728 0.0 gi|340628016|ref|YP_004746468.1| putative tRNA [Mycobacterium ca... 724 0.0 gi|289755139|ref|ZP_06514517.1| tRNA (5-methylaminomethyl-2-thio... 721 0.0 gi|289571223|ref|ZP_06451450.1| LOW QUALITY PROTEIN: tRNA (5-met... 694 0.0 gi|240169595|ref|ZP_04748254.1| tRNA (5-methylaminomethyl-2-thio... 640 0.0 gi|118617523|ref|YP_905855.1| tRNA-specific 2-thiouridylase MnmA... 624 8e-177 gi|342858154|ref|ZP_08714809.1| tRNA-specific 2-thiouridylase Mn... 618 4e-175 gi|41409155|ref|NP_961991.1| tRNA (5-methylaminomethyl-2-thiouri... 618 5e-175 gi|205821882|sp|A0QJE5.2|MNMA_MYCA1 RecName: Full=tRNA-specific ... 617 9e-175 gi|254820453|ref|ZP_05225454.1| tRNA-specific 2-thiouridylase Mn... 617 1e-174 gi|254776291|ref|ZP_05217807.1| tRNA-specific 2-thiouridylase Mn... 615 3e-174 gi|15827913|ref|NP_302176.1| tRNA-specific 2-thiouridylase MnmA ... 615 3e-174 gi|183981704|ref|YP_001849995.1| tRNA (5-methylaminomethyl-2-thi... 613 2e-173 gi|296171256|ref|ZP_06852660.1| tRNA (5-methylaminomethyl-2-thio... 612 4e-173 gi|336459166|gb|EGO38113.1| tRNA (5-methylaminomethyl-2-thiourid... 608 3e-172 gi|118467226|ref|YP_883033.1| tRNA-specific 2-thiouridylase MnmA... 607 1e-171 gi|120403098|ref|YP_952927.1| tRNA-specific 2-thiouridylase MnmA... 584 7e-165 gi|145224833|ref|YP_001135511.1| tRNA-specific 2-thiouridylase M... 578 7e-163 gi|333991381|ref|YP_004523995.1| tRNA (5-methylaminomethyl-2-thi... 577 9e-163 gi|126434444|ref|YP_001070135.1| tRNA-specific 2-thiouridylase M... 568 6e-160 gi|108798844|ref|YP_639041.1| tRNA-specific 2-thiouridylase MnmA... 566 1e-159 gi|118473593|ref|YP_886701.1| tRNA-specific 2-thiouridylase MnmA... 551 8e-155 gi|169630437|ref|YP_001704086.1| tRNA-specific 2-thiouridylase M... 533 2e-149 gi|54026238|ref|YP_120480.1| tRNA-specific 2-thiouridylase MnmA ... 511 6e-143 gi|325675868|ref|ZP_08155552.1| tRNA (5-methylaminomethyl-2-thio... 511 9e-143 gi|226305865|ref|YP_002765825.1| tRNA uridine-sulfurtransferase ... 506 2e-141 gi|111023428|ref|YP_706400.1| tRNA-specific 2-thiouridylase MnmA... 504 7e-141 gi|226365929|ref|YP_002783712.1| tRNA-specific 2-thiouridylase M... 504 1e-140 gi|333920880|ref|YP_004494461.1| tRNA-specific 2-thiouridylase m... 499 3e-139 gi|312140513|ref|YP_004007849.1| tRNA (5-methylaminomethyl-2-thi... 492 4e-137 gi|298526491|ref|ZP_07013900.1| tRNA (5-methylaminomethyl-2-thio... 483 2e-134 gi|289575733|ref|ZP_06455960.1| LOW QUALITY PROTEIN: tRNA (5-met... 479 4e-133 gi|296140603|ref|YP_003647846.1| tRNA(5-methylaminomethyl-2-thio... 471 1e-130 gi|262203144|ref|YP_003274352.1| tRNA(5-methylaminomethyl-2-thio... 470 1e-130 gi|326381518|ref|ZP_08203212.1| tRNA-specific 2-thiouridylase Mn... 463 2e-128 gi|343926874|ref|ZP_08766367.1| tRNA-specific 2-thiouridylase Mn... 450 1e-124 gi|237785842|ref|YP_002906547.1| tRNA-specific 2-thiouridylase M... 449 3e-124 gi|340794771|ref|YP_004760234.1| tRNA 5-methylaminomethyl-2-thio... 436 3e-120 gi|68536405|ref|YP_251110.1| tRNA (5-methylaminomethyl-2-thiouri... 431 1e-118 gi|205822051|sp|A4FMT5.2|MNMA_SACEN RecName: Full=tRNA-specific ... 430 2e-118 gi|257054918|ref|YP_003132750.1| tRNA-specific 2-thiouridylase M... 430 2e-118 gi|256380038|ref|YP_003103698.1| tRNA-specific 2-thiouridylase M... 427 2e-117 gi|319949296|ref|ZP_08023374.1| tRNA-specific 2-thiouridylase Mn... 424 9e-117 gi|296119548|ref|ZP_06838106.1| tRNA (5-methylaminomethyl-2-thio... 424 1e-116 gi|260577537|ref|ZP_05845477.1| probable tRNA (5-methylaminometh... 423 2e-116 gi|302865761|ref|YP_003834398.1| tRNA (5-methylaminomethyl-2-thi... 422 5e-116 gi|255324545|ref|ZP_05365662.1| tRNA-methyltransferase [Coryneba... 421 7e-116 gi|291004692|ref|ZP_06562665.1| tRNA-specific 2-thiouridylase Mn... 421 7e-116 >gi|15610161|ref|NP_217540.1| tRNA-specific 2-thiouridylase MnmA [Mycobacterium tuberculosis H37Rv] gi|15842587|ref|NP_337624.1| tRNA-specific 2-thiouridylase MnmA [Mycobacterium tuberculosis CDC1551] gi|31794202|ref|NP_856695.1| tRNA-specific 2-thiouridylase MnmA [Mycobacterium bovis AF2122/97] 66 more sequence titles Length=367 Score = 732 bits (1889), Expect = 0.0, Method: Compositional matrix adjust. Identities = 367/367 (100%), Positives = 367/367 (100%), Gaps = 0/367 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV Sbjct 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD Sbjct 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG Sbjct 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL Sbjct 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 Query 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV Sbjct 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 Query 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGASGLS 360 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGASGLS Sbjct 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGASGLS 360 Query 361 TGGNPGA 367 TGGNPGA Sbjct 361 TGGNPGA 367 >gi|339633032|ref|YP_004724674.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase TRMU [Mycobacterium africanum GM041182] gi|339332388|emb|CCC28101.1| putative tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase TRMU [Mycobacterium africanum GM041182] Length=367 Score = 730 bits (1885), Expect = 0.0, Method: Compositional matrix adjust. Identities = 366/367 (99%), Positives = 367/367 (100%), Gaps = 0/367 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV Sbjct 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD Sbjct 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG Sbjct 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 LAVANKPDSHDICFIPSGNT+AFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL Sbjct 181 LAVANKPDSHDICFIPSGNTRAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 Query 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV Sbjct 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 Query 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGASGLS 360 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGASGLS Sbjct 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGASGLS 360 Query 361 TGGNPGA 367 TGGNPGA Sbjct 361 TGGNPGA 367 >gi|167970222|ref|ZP_02552499.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Mycobacterium tuberculosis H37Ra] Length=367 Score = 728 bits (1880), Expect = 0.0, Method: Compositional matrix adjust. Identities = 366/367 (99%), Positives = 366/367 (99%), Gaps = 0/367 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMAL TAPGTLRTGSRGCCSKEDAADARRV Sbjct 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALWTAPGTLRTGSRGCCSKEDAADARRV 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD Sbjct 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG Sbjct 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL Sbjct 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 Query 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV Sbjct 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 Query 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGASGLS 360 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGASGLS Sbjct 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGASGLS 360 Query 361 TGGNPGA 367 TGGNPGA Sbjct 361 TGGNPGA 367 >gi|340628016|ref|YP_004746468.1| putative tRNA [Mycobacterium canettii CIPT 140010059] gi|340006206|emb|CCC45380.1| putative tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase TRMU [Mycobacterium canettii CIPT 140010059] Length=367 Score = 724 bits (1868), Expect = 0.0, Method: Compositional matrix adjust. Identities = 363/367 (99%), Positives = 364/367 (99%), Gaps = 0/367 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV Sbjct 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD Sbjct 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLR AAFPIGDTPKRQIRAEAARRG Sbjct 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRRAAFPIGDTPKRQIRAEAARRG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQR+GL Sbjct 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRKGL 240 Query 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 GIAGPGPNGR RYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV Sbjct 241 GIAGPGPNGRARYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 Query 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGASGLS 360 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAG SGLS Sbjct 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGTSGLS 360 Query 361 TGGNPGA 367 TGGNPGA Sbjct 361 TGGNPGA 367 >gi|289755139|ref|ZP_06514517.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase TrmU [Mycobacterium tuberculosis EAS054] gi|308375963|ref|ZP_07445668.2| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase trmU [Mycobacterium tuberculosis SUMu007] gi|308377199|ref|ZP_07441475.2| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase trmU [Mycobacterium tuberculosis SUMu008] gi|289695726|gb|EFD63155.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase TrmU [Mycobacterium tuberculosis EAS054] gi|308344583|gb|EFP33434.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase trmU [Mycobacterium tuberculosis SUMu007] gi|308348530|gb|EFP37381.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase trmU [Mycobacterium tuberculosis SUMu008] Length=361 Score = 721 bits (1861), Expect = 0.0, Method: Compositional matrix adjust. Identities = 361/361 (100%), Positives = 361/361 (100%), Gaps = 0/361 (0%) Query 7 MSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRVADVLGI 66 MSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRVADVLGI Sbjct 1 MSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRVADVLGI 60 Query 67 PFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFDTVATGH 126 PFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFDTVATGH Sbjct 61 PFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFDTVATGH 120 Query 127 YARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRGLAVANK 186 YARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRGLAVANK Sbjct 121 YARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRGLAVANK 180 Query 187 PDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGLGIAGPG 246 PDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGLGIAGPG Sbjct 181 PDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGLGIAGPG 240 Query 247 PNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQVRAHGET 306 PNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQVRAHGET Sbjct 241 PNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQVRAHGET 300 Query 307 VSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGASGLSTGGNPG 366 VSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGASGLSTGGNPG Sbjct 301 VSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGASGLSTGGNPG 360 Query 367 A 367 A Sbjct 361 A 361 >gi|289571223|ref|ZP_06451450.1| LOW QUALITY PROTEIN: tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase trmU [Mycobacterium tuberculosis T17] gi|289544977|gb|EFD48625.1| LOW QUALITY PROTEIN: tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase trmU [Mycobacterium tuberculosis T17] Length=347 Score = 694 bits (1792), Expect = 0.0, Method: Compositional matrix adjust. Identities = 347/347 (100%), Positives = 347/347 (100%), Gaps = 0/347 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV Sbjct 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD Sbjct 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG Sbjct 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL Sbjct 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 Query 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV Sbjct 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 Query 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEV 347 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEV Sbjct 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEV 347 >gi|240169595|ref|ZP_04748254.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Mycobacterium kansasii ATCC 12478] Length=363 Score = 640 bits (1651), Expect = 0.0, Method: Compositional matrix adjust. Identities = 317/356 (90%), Positives = 337/356 (95%), Gaps = 0/356 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVH+ALSTAP TLRTGSRGCCSKEDA+DARRV Sbjct 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHLALSTAPSTLRTGSRGCCSKEDASDARRV 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVLGIPFYVWDFAEKF EDVI+DFVSSYARGETPNPCVRCNQ+IKF+ALSARA+ALGFD Sbjct 61 ADVLGIPFYVWDFAEKFNEDVIDDFVSSYARGETPNPCVRCNQRIKFSALSARALALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 VATGHYARLS GRLRRAVDRDKDQSYVLAVLTA+QLRHA FPIGDTPK QIRAEAARRG Sbjct 121 AVATGHYARLSDGRLRRAVDRDKDQSYVLAVLTARQLRHALFPIGDTPKPQIRAEAARRG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 LAVA+KPDSHDICFIPSG+T+AFLG RIGVRRG+VVDADG VLA+HDGVH FTIGQR+GL Sbjct 181 LAVADKPDSHDICFIPSGDTRAFLGARIGVRRGLVVDADGAVLATHDGVHEFTIGQRKGL 240 Query 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 GI GPGP+GRPRYVTAIDA+TATV VGDV+DLDV LTGRAPVFTAGAAPSGPVDC VQV Sbjct 241 GIPGPGPDGRPRYVTAIDAETATVRVGDVSDLDVHALTGRAPVFTAGAAPSGPVDCAVQV 300 Query 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGA 356 RAHGETV+A AEL+GD L V+LHAPLRGVARGQT VLYRPDP GDEVLGSATIAG+ Sbjct 301 RAHGETVAATAELVGDELLVRLHAPLRGVARGQTAVLYRPDPGGDEVLGSATIAGS 356 >gi|118617523|ref|YP_905855.1| tRNA-specific 2-thiouridylase MnmA [Mycobacterium ulcerans Agy99] gi|166235069|sp|A0PPW5.1|MNMA_MYCUA RecName: Full=tRNA-specific 2-thiouridylase mnmA gi|205822174|sp|B2HIE6.2|MNMA_MYCMM RecName: Full=tRNA-specific 2-thiouridylase mnmA gi|118569633|gb|ABL04384.1| tRNA (5-methylaminomethyl-2-thiouridylate)- methyltransferase TrmU [Mycobacterium ulcerans Agy99] Length=363 Score = 624 bits (1609), Expect = 8e-177, Method: Compositional matrix adjust. Identities = 304/360 (85%), Positives = 330/360 (92%), Gaps = 0/360 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 MKVLAAMSGGVDSSVAAARMVDAGH+VVGVH+ALSTAPG LRTGSRGCCSKEDA+DARRV Sbjct 1 MKVLAAMSGGVDSSVAAARMVDAGHDVVGVHLALSTAPGALRTGSRGCCSKEDASDARRV 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVLGIPFYVWDFAE+FKEDVI+DFVSSYARGETPNPC+RCNQ+IKF+AL A+A+ALGFD Sbjct 61 ADVLGIPFYVWDFAERFKEDVIDDFVSSYARGETPNPCIRCNQRIKFSALYAKALALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 V TGHYARLS GRLRRAVD+DKDQSYVLAVLTA+QLRHA FPIGDTPK QIR EA+RRG Sbjct 121 VVVTGHYARLSEGRLRRAVDQDKDQSYVLAVLTAEQLRHAMFPIGDTPKPQIREEASRRG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 LAVA+KPDSHDICFIPSGNT+ FLGERIGVRRG VVDA G VLA+HDGVHGFTIGQR+GL Sbjct 181 LAVADKPDSHDICFIPSGNTQTFLGERIGVRRGTVVDAAGAVLATHDGVHGFTIGQRKGL 240 Query 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 GI GPGP+GRPRYVTAIDA+T TV VGDV DL V LTGRAP+FTAG AP+GP++C VQV Sbjct 241 GIPGPGPDGRPRYVTAIDAETGTVRVGDVADLQVHALTGRAPIFTAGTAPTGPLECAVQV 300 Query 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGASGLS 360 RAHGET SAVAEL+GD L V+LH+PLRGVARGQTLVLYRPDP GDEVLGSATI S S Sbjct 301 RAHGETTSAVAELVGDELSVRLHSPLRGVARGQTLVLYRPDPDGDEVLGSATITATSACS 360 >gi|342858154|ref|ZP_08714809.1| tRNA-specific 2-thiouridylase MnmA [Mycobacterium colombiense CECT 3035] gi|342133858|gb|EGT87038.1| tRNA-specific 2-thiouridylase MnmA [Mycobacterium colombiense CECT 3035] Length=358 Score = 618 bits (1594), Expect = 4e-175, Method: Compositional matrix adjust. Identities = 299/357 (84%), Positives = 330/357 (93%), Gaps = 0/357 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 MKVLAAMSGGVDSSVAAARMVDAGH+VVGVH+ALS+APGTLRTGSRGCCSKEDA+DARRV Sbjct 1 MKVLAAMSGGVDSSVAAARMVDAGHDVVGVHLALSSAPGTLRTGSRGCCSKEDASDARRV 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVLGIPFYVWDFAE+FK DVI++FVSSYARGETPNPCV CNQ+IKF+ALSA+AVALGFD Sbjct 61 ADVLGIPFYVWDFAERFKADVIDEFVSSYARGETPNPCVVCNQKIKFSALSAKAVALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 TVATGHYARLS GRLRRAVD KDQSYVLAVL+A+QLR AAFPIGDTPK QIRAEAARRG Sbjct 121 TVATGHYARLSNGRLRRAVDHGKDQSYVLAVLSAEQLRRAAFPIGDTPKPQIRAEAARRG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 L VA KPDSHDICFIPSGNT+AFLGERIGVRRG VV+ADG VLA HDGVHGFT+GQR+GL Sbjct 181 LTVAEKPDSHDICFIPSGNTRAFLGERIGVRRGAVVNADGTVLAEHDGVHGFTVGQRKGL 240 Query 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 GIAGPGP+GRPRYVTAIDA+T+TVHVG+ DL+++ + GRAPVFT+G PSGPV+C VQV Sbjct 241 GIAGPGPDGRPRYVTAIDAETSTVHVGEAADLEIREMVGRAPVFTSGVTPSGPVECAVQV 300 Query 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGAS 357 RAHGET A+AEL+ D L V+L AP+RGVARGQTLVLYRPDP GDEVLGSATIAG + Sbjct 301 RAHGETADAIAELVDDELVVRLRAPMRGVARGQTLVLYRPDPEGDEVLGSATIAGTA 357 >gi|41409155|ref|NP_961991.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Mycobacterium avium subsp. paratuberculosis K-10] gi|81571098|sp|Q73VF7.1|MNMA_MYCPA RecName: Full=tRNA-specific 2-thiouridylase mnmA gi|41397975|gb|AAS05605.1| hypothetical protein MAP_3057c [Mycobacterium avium subsp. paratuberculosis K-10] Length=358 Score = 618 bits (1593), Expect = 5e-175, Method: Compositional matrix adjust. Identities = 304/357 (86%), Positives = 330/357 (93%), Gaps = 0/357 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 M+VLAAMSGGVDSSVAAARMVDAGH+VVGVH+ALSTAPGTLRTGSRGCCSKEDA+DARRV Sbjct 1 MRVLAAMSGGVDSSVAAARMVDAGHDVVGVHLALSTAPGTLRTGSRGCCSKEDASDARRV 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVLGIPFYVWDFAEKF+ DVI++F+S+YARGETPNPCV CNQ+IKF+ALSA+AVALGFD Sbjct 61 ADVLGIPFYVWDFAEKFQADVIDEFLSAYARGETPNPCVTCNQKIKFSALSAKAVALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 VATGHYARLSGGRLRRAVD+DKDQSYVLAVL+A+QLRHAAFPIGDTPK QIR EAARRG Sbjct 121 AVATGHYARLSGGRLRRAVDKDKDQSYVLAVLSAEQLRHAAFPIGDTPKPQIREEAARRG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 LAVA KPDSHDICFIPSGNT+AFLGERIGVRRG VVDADG VLA HDGVHGFTIGQR+GL Sbjct 181 LAVAEKPDSHDICFIPSGNTRAFLGERIGVRRGAVVDADGTVLAEHDGVHGFTIGQRKGL 240 Query 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 GIAGPGP+GRPRYVTAIDADTATV VG+ +DLDV+ + GRA VFT+G APSGP +C VQV Sbjct 241 GIAGPGPDGRPRYVTAIDADTATVRVGEASDLDVREMLGRAVVFTSGTAPSGPFECAVQV 300 Query 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGAS 357 RAHGET AVAEL+GD L V+L APLRGVA GQTL LYR DP GDEVLGSATIAG S Sbjct 301 RAHGETADAVAELVGDELVVRLRAPLRGVAPGQTLALYRRDPDGDEVLGSATIAGTS 357 >gi|205821882|sp|A0QJE5.2|MNMA_MYCA1 RecName: Full=tRNA-specific 2-thiouridylase mnmA Length=358 Score = 617 bits (1591), Expect = 9e-175, Method: Compositional matrix adjust. Identities = 304/357 (86%), Positives = 329/357 (93%), Gaps = 0/357 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 M+VLAAMSGGVDSSVAAARMVDAGH+VVGVH+ALSTAPGTLRTGSRGCCSKEDA+DARRV Sbjct 1 MRVLAAMSGGVDSSVAAARMVDAGHDVVGVHLALSTAPGTLRTGSRGCCSKEDASDARRV 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVLGIPFYVWDFAEKF+ DVI++FVS+YARGETPNPCV CNQ+IKF+ALSA+AVALGFD Sbjct 61 ADVLGIPFYVWDFAEKFQADVIDEFVSAYARGETPNPCVTCNQKIKFSALSAKAVALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 VATGHYARLSGGRLRRAVD+DKDQSYVLAVL+A+QLRHAAFPIGDTPK QIR EAARRG Sbjct 121 AVATGHYARLSGGRLRRAVDKDKDQSYVLAVLSAEQLRHAAFPIGDTPKPQIREEAARRG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 LAVA KPDSHDICFIPSGNT+AFLGERIGVRRG VV+ADG VLA HDGVHGFTIGQR+GL Sbjct 181 LAVAEKPDSHDICFIPSGNTRAFLGERIGVRRGAVVNADGTVLAEHDGVHGFTIGQRKGL 240 Query 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 GIAGPGP+GRPRYVTAIDADTATV VG+ DLDV+ + GRA VFT+G APSGP +C VQV Sbjct 241 GIAGPGPDGRPRYVTAIDADTATVRVGEAADLDVREMLGRAVVFTSGTAPSGPFECAVQV 300 Query 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGAS 357 RAHGET AVAEL+GD L V+L APLRGVA GQTL LYR DP GDEVLGSATIAG S Sbjct 301 RAHGETADAVAELVGDELVVRLRAPLRGVAPGQTLALYRHDPDGDEVLGSATIAGTS 357 >gi|254820453|ref|ZP_05225454.1| tRNA-specific 2-thiouridylase MnmA [Mycobacterium intracellulare ATCC 13950] Length=358 Score = 617 bits (1590), Expect = 1e-174, Method: Compositional matrix adjust. Identities = 302/357 (85%), Positives = 330/357 (93%), Gaps = 0/357 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 MKVLAAMSGGVDSSVAAARMVDAGH+VVGVH+ALS++PGTLR+GSRGCCSKEDA+DARRV Sbjct 1 MKVLAAMSGGVDSSVAAARMVDAGHDVVGVHLALSSSPGTLRSGSRGCCSKEDASDARRV 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVLGIPFYVWDFAEKF+ DVI++FVSSYARGETPNPCV CNQ+IKF+ALSARAVALGFD Sbjct 61 ADVLGIPFYVWDFAEKFQADVIDEFVSSYARGETPNPCVVCNQKIKFSALSARAVALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 TVATGHYARLSGGRLRRAVD DKDQSYVLAVL+A+QLRHAAFPIGDTPK QIRAEAARRG Sbjct 121 TVATGHYARLSGGRLRRAVDHDKDQSYVLAVLSAEQLRHAAFPIGDTPKSQIRAEAARRG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 LAVA KPDSHDICFIPSG+T+AFLG RIGVRRG VV+ADG VLA HDGVHGFTIGQR+GL Sbjct 181 LAVAEKPDSHDICFIPSGDTRAFLGSRIGVRRGAVVNADGTVLAEHDGVHGFTIGQRKGL 240 Query 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 GIAGPGP+GRPRYVTAIDA+TATV VGDV +L+V+ LTGRAPVFT+G PSGP++C VQV Sbjct 241 GIAGPGPDGRPRYVTAIDAETATVRVGDVAELEVRELTGRAPVFTSGVTPSGPIECAVQV 300 Query 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGAS 357 RAHGET AVAEL+G L V+L PLRGVA GQTLVLYRPDP GDEV+GSA IA S Sbjct 301 RAHGETADAVAELVGGELVVRLRVPLRGVAPGQTLVLYRPDPDGDEVIGSAVIAATS 357 >gi|254776291|ref|ZP_05217807.1| tRNA-specific 2-thiouridylase MnmA [Mycobacterium avium subsp. avium ATCC 25291] Length=358 Score = 615 bits (1587), Expect = 3e-174, Method: Compositional matrix adjust. Identities = 304/357 (86%), Positives = 328/357 (92%), Gaps = 0/357 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 M+VLAAMSGGVDSSVAAARMVDAGH+VVGVH+ALSTAPGTLRTGSRGCCSKEDA+DARRV Sbjct 1 MRVLAAMSGGVDSSVAAARMVDAGHDVVGVHLALSTAPGTLRTGSRGCCSKEDASDARRV 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVLGIPFYVWDFAEKF+ DVI+ FVS+YARGETPNPCV CNQ+IKF+ALSA+AVALGFD Sbjct 61 ADVLGIPFYVWDFAEKFQADVIDAFVSAYARGETPNPCVTCNQKIKFSALSAKAVALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 VATGHYARLSGGRLRRAVD+DKDQSYVLAVL+A+QLRHAAFPIGDTPK QIR EAARRG Sbjct 121 AVATGHYARLSGGRLRRAVDKDKDQSYVLAVLSAEQLRHAAFPIGDTPKPQIREEAARRG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 LAVA KPDSHDICFIPSGNT+AFLGERIGVRRG VV+ADG VLA HDGVHGFTIGQR+GL Sbjct 181 LAVAEKPDSHDICFIPSGNTRAFLGERIGVRRGAVVNADGTVLAEHDGVHGFTIGQRKGL 240 Query 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 GIAGPGP+GRPRYVTAIDADTATV VG+ DLDV+ + GRA VFT+G APSGP +C VQV Sbjct 241 GIAGPGPDGRPRYVTAIDADTATVRVGEAADLDVREMLGRAVVFTSGTAPSGPFECAVQV 300 Query 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGAS 357 RAHGET AVAEL+GD L V+L APLRGVA GQTL LYR DP GDEVLGSATIAG S Sbjct 301 RAHGETADAVAELVGDELVVRLRAPLRGVAPGQTLALYRRDPDGDEVLGSATIAGTS 357 >gi|15827913|ref|NP_302176.1| tRNA-specific 2-thiouridylase MnmA [Mycobacterium leprae TN] gi|221230390|ref|YP_002503806.1| tRNA-specific 2-thiouridylase MnmA [Mycobacterium leprae Br4923] gi|3122976|sp|O33099.1|MNMA_MYCLE RecName: Full=tRNA-specific 2-thiouridylase mnmA gi|254811455|sp|B8ZS29.1|MNMA_MYCLB RecName: Full=tRNA-specific 2-thiouridylase mnmA gi|2414533|emb|CAB16422.1| hypothetical protein MLCB637.07 [Mycobacterium leprae] gi|13093466|emb|CAC30660.1| probable tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Mycobacterium leprae] gi|219933497|emb|CAR71802.1| probable tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Mycobacterium leprae Br4923] Length=358 Score = 615 bits (1587), Expect = 3e-174, Method: Compositional matrix adjust. Identities = 305/357 (86%), Positives = 325/357 (92%), Gaps = 0/357 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 MKVLAAMSGGVDSSVAAARMVDAGH+VVGVH+ALST GTLRTGSRGCCSKED +DARRV Sbjct 1 MKVLAAMSGGVDSSVAAARMVDAGHDVVGVHLALSTVSGTLRTGSRGCCSKEDVSDARRV 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVLGIPFYVWDFAEKF+EDVI++FVS+YARGETPNPCV+CNQ+IKF+ALS RA+ LGFD Sbjct 61 ADVLGIPFYVWDFAEKFQEDVIDNFVSAYARGETPNPCVQCNQRIKFSALSVRALMLGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 TVATGHYARLSGGRLRRAVDR KDQSYVLAVLTAQQLRHAAFPIGDT KRQ+RAEAARRG Sbjct 121 TVATGHYARLSGGRLRRAVDRGKDQSYVLAVLTAQQLRHAAFPIGDTLKRQVRAEAARRG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 L VA+KPDSH ICFIPSGNT+AFL E IG+RRG VVDADG VLA HDGVHGFT+GQR+GL Sbjct 181 LLVADKPDSHGICFIPSGNTRAFLSECIGIRRGSVVDADGTVLAEHDGVHGFTVGQRKGL 240 Query 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 GI GPGPNGRPRYVTAIDADTATV+VGD DL V TL GRAPVFTAGAAP GPV CVVQ+ Sbjct 241 GIVGPGPNGRPRYVTAIDADTATVYVGDAADLGVHTLIGRAPVFTAGAAPLGPVQCVVQI 300 Query 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGAS 357 RAHGET AV ELIGD LFV+L PLRGVARGQTLVLYR DP GDEVLGSATIA S Sbjct 301 RAHGETAVAVVELIGDELFVRLCEPLRGVARGQTLVLYRLDPDGDEVLGSATIASMS 357 >gi|183981704|ref|YP_001849995.1| tRNA (5-methylaminomethyl-2-thiouridylate)- methyltransferase TrmU [Mycobacterium marinum M] gi|183175030|gb|ACC40140.1| tRNA (5-methylaminomethyl-2-thiouridylate)- methyltransferase TrmU [Mycobacterium marinum M] Length=357 Score = 613 bits (1580), Expect = 2e-173, Method: Compositional matrix adjust. Identities = 298/354 (85%), Positives = 324/354 (92%), Gaps = 0/354 (0%) Query 7 MSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRVADVLGI 66 MSGGVDSSVAAARMVDAGH+VVGVH+ALSTAPG LRTGSRGCCSKEDA+DARRVADVLGI Sbjct 1 MSGGVDSSVAAARMVDAGHDVVGVHLALSTAPGALRTGSRGCCSKEDASDARRVADVLGI 60 Query 67 PFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFDTVATGH 126 PFYVWDFAE+FKEDVI+DFVSSYARGETPNPC+RCNQ+IKF+AL A+A+ALGFD V TGH Sbjct 61 PFYVWDFAERFKEDVIDDFVSSYARGETPNPCIRCNQRIKFSALYAKALALGFDVVVTGH 120 Query 127 YARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRGLAVANK 186 YARLS GRLRRAVD+DKDQSYVLAVLTA+QLRHA FPIGDTPK QIR EA+RRGLAVA+K Sbjct 121 YARLSEGRLRRAVDQDKDQSYVLAVLTAEQLRHAMFPIGDTPKPQIREEASRRGLAVADK 180 Query 187 PDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGLGIAGPG 246 PDSHDICFIPSGNT+ FLGERIGVRRG VVDA G VLA+HDGVHGFTIGQR+GLGI GPG Sbjct 181 PDSHDICFIPSGNTQTFLGERIGVRRGTVVDAAGAVLATHDGVHGFTIGQRKGLGIPGPG 240 Query 247 PNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQVRAHGET 306 P+GRPRYVTAIDA+T TV VGDV DL V LTGRAP+FTAG AP+GP++C VQVRAHGET Sbjct 241 PDGRPRYVTAIDAETGTVRVGDVADLQVHALTGRAPIFTAGTAPTGPLECAVQVRAHGET 300 Query 307 VSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGASGLS 360 SAVAEL+GD L V+LH+PLRGVARGQTLVLYRPDP GDEVLGSATI S S Sbjct 301 TSAVAELVGDELSVRLHSPLRGVARGQTLVLYRPDPDGDEVLGSATITATSACS 354 >gi|296171256|ref|ZP_06852660.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Mycobacterium parascrofulaceum ATCC BAA-614] gi|295894224|gb|EFG73981.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Mycobacterium parascrofulaceum ATCC BAA-614] Length=358 Score = 612 bits (1577), Expect = 4e-173, Method: Compositional matrix adjust. Identities = 303/357 (85%), Positives = 325/357 (92%), Gaps = 0/357 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 MK+LAAMSGGVDSSVAAARMVDAGH+VVGVH+ALS+APG LRTGSRGCCSKEDA+DA RV Sbjct 1 MKILAAMSGGVDSSVAAARMVDAGHDVVGVHLALSSAPGALRTGSRGCCSKEDASDASRV 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVLGIPFYVWDFAE+F+ DVI+DFVSSYARGETPNPCVRCNQ+IKF+ALSA+A+ALGFD Sbjct 61 ADVLGIPFYVWDFAERFQADVIDDFVSSYARGETPNPCVRCNQRIKFSALSAKALALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 TVATGHYARLSGGRLRRAVD DKDQSYVLAVLTA+QLRHA FPIGDTPK QIRAEAARRG Sbjct 121 TVATGHYARLSGGRLRRAVDHDKDQSYVLAVLTAEQLRHATFPIGDTPKAQIRAEAARRG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 LAVA KPDSHDICFIPSG+T+AFLGERI VRRG VV ADG VLA HDGVHGFT+GQR+GL Sbjct 181 LAVAAKPDSHDICFIPSGDTRAFLGERIAVRRGAVVGADGAVLAQHDGVHGFTVGQRKGL 240 Query 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 GIAGPGP+GRPRYVT IDADTATV VGD DLDV L GR PVFTAG PSG V+C VQV Sbjct 241 GIAGPGPDGRPRYVTEIDADTATVRVGDAADLDVLELRGREPVFTAGRPPSGLVECAVQV 300 Query 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGAS 357 RAHGET AVAEL+GD L V+L PLRGVARGQTLVLYRPDP GDEVLGSATIAG + Sbjct 301 RAHGETAPAVAELVGDELIVRLRVPLRGVARGQTLVLYRPDPDGDEVLGSATIAGTA 357 >gi|336459166|gb|EGO38113.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Mycobacterium avium subsp. paratuberculosis S397] Length=352 Score = 608 bits (1569), Expect = 3e-172, Method: Compositional matrix adjust. Identities = 299/351 (86%), Positives = 324/351 (93%), Gaps = 0/351 (0%) Query 7 MSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRVADVLGI 66 MSGGVDSSVAAARMVDAGH+VVGVH+ALSTAPGTLRTGSRGCCSKEDA+DARRVADVLGI Sbjct 1 MSGGVDSSVAAARMVDAGHDVVGVHLALSTAPGTLRTGSRGCCSKEDASDARRVADVLGI 60 Query 67 PFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFDTVATGH 126 PFYVWDFAEKF+ DVI++F+S+YARGETPNPCV CNQ+IKF+ALSA+AVALGFD VATGH Sbjct 61 PFYVWDFAEKFQADVIDEFLSAYARGETPNPCVTCNQKIKFSALSAKAVALGFDAVATGH 120 Query 127 YARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRGLAVANK 186 YARLSGGRLRRAVD+DKDQSYVLAVL+A+QLRHAAFPIGDTPK QIR EAARRGLAVA K Sbjct 121 YARLSGGRLRRAVDKDKDQSYVLAVLSAEQLRHAAFPIGDTPKPQIREEAARRGLAVAEK 180 Query 187 PDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGLGIAGPG 246 PDSHDICFIPSGNT+AFLGERIGVRRG VVDADG VLA HDGVHGFTIGQR+GLGIAGPG Sbjct 181 PDSHDICFIPSGNTRAFLGERIGVRRGAVVDADGTVLAEHDGVHGFTIGQRKGLGIAGPG 240 Query 247 PNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQVRAHGET 306 P+GRPRYVTAIDADTATV VG+ +DLDV+ + GRA VFT+G APSGP +C VQVRAHGET Sbjct 241 PDGRPRYVTAIDADTATVRVGEASDLDVREMLGRAVVFTSGTAPSGPFECAVQVRAHGET 300 Query 307 VSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGAS 357 AVAEL+GD L V+L APLRGVA GQTL LYR DP GDEVLGSATIAG S Sbjct 301 ADAVAELVGDELVVRLRAPLRGVAPGQTLALYRRDPDGDEVLGSATIAGTS 351 >gi|118467226|ref|YP_883033.1| tRNA-specific 2-thiouridylase MnmA [Mycobacterium avium 104] gi|118168513|gb|ABK69410.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Mycobacterium avium 104] Length=352 Score = 607 bits (1564), Expect = 1e-171, Method: Compositional matrix adjust. Identities = 299/351 (86%), Positives = 323/351 (93%), Gaps = 0/351 (0%) Query 7 MSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRVADVLGI 66 MSGGVDSSVAAARMVDAGH+VVGVH+ALSTAPGTLRTGSRGCCSKEDA+DARRVADVLGI Sbjct 1 MSGGVDSSVAAARMVDAGHDVVGVHLALSTAPGTLRTGSRGCCSKEDASDARRVADVLGI 60 Query 67 PFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFDTVATGH 126 PFYVWDFAEKF+ DVI++FVS+YARGETPNPCV CNQ+IKF+ALSA+AVALGFD VATGH Sbjct 61 PFYVWDFAEKFQADVIDEFVSAYARGETPNPCVTCNQKIKFSALSAKAVALGFDAVATGH 120 Query 127 YARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRGLAVANK 186 YARLSGGRLRRAVD+DKDQSYVLAVL+A+QLRHAAFPIGDTPK QIR EAARRGLAVA K Sbjct 121 YARLSGGRLRRAVDKDKDQSYVLAVLSAEQLRHAAFPIGDTPKPQIREEAARRGLAVAEK 180 Query 187 PDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGLGIAGPG 246 PDSHDICFIPSGNT+AFLGERIGVRRG VV+ADG VLA HDGVHGFTIGQR+GLGIAGPG Sbjct 181 PDSHDICFIPSGNTRAFLGERIGVRRGAVVNADGTVLAEHDGVHGFTIGQRKGLGIAGPG 240 Query 247 PNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQVRAHGET 306 P+GRPRYVTAIDADTATV VG+ DLDV+ + GRA VFT+G APSGP +C VQVRAHGET Sbjct 241 PDGRPRYVTAIDADTATVRVGEAADLDVREMLGRAVVFTSGTAPSGPFECAVQVRAHGET 300 Query 307 VSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGAS 357 AVAEL+GD L V+L APLRGVA GQTL LYR DP GDEVLGSATIAG S Sbjct 301 ADAVAELVGDELVVRLRAPLRGVAPGQTLALYRHDPDGDEVLGSATIAGTS 351 >gi|120403098|ref|YP_952927.1| tRNA-specific 2-thiouridylase MnmA [Mycobacterium vanbaalenii PYR-1] gi|166235070|sp|A1T6X1.1|MNMA_MYCVP RecName: Full=tRNA-specific 2-thiouridylase mnmA gi|119955916|gb|ABM12921.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Mycobacterium vanbaalenii PYR-1] Length=357 Score = 584 bits (1506), Expect = 7e-165, Method: Compositional matrix adjust. Identities = 291/356 (82%), Positives = 316/356 (89%), Gaps = 0/356 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 M+VL AMSGGVDSSVAAARMVDAGH+VVGVH+ALS+APGTLRTGSRGCCSKEDA DARRV Sbjct 1 MRVLVAMSGGVDSSVAAARMVDAGHDVVGVHLALSSAPGTLRTGSRGCCSKEDAGDARRV 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVLGIPFYVWDFA++FKEDVI+DFV SYARGETPNPCVRCN++IKF+ALSARA+ALGFD Sbjct 61 ADVLGIPFYVWDFADRFKEDVIDDFVESYARGETPNPCVRCNERIKFSALSARALALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 +ATGHYARL+ GRLRRAVD DKDQSYVLAVLTA+QLRHA FPIGDTPK IR EAARRG Sbjct 121 ALATGHYARLTDGRLRRAVDHDKDQSYVLAVLTAEQLRHAVFPIGDTPKPAIREEAARRG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 LAVA+K DSHDICFIPSG+T+AFLG RIGVRRG VVDA G VLA HDGVHGFTIGQR+GL Sbjct 181 LAVADKADSHDICFIPSGDTRAFLGARIGVRRGSVVDATGAVLAEHDGVHGFTIGQRKGL 240 Query 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 GI GPGP+GRPRYVT IDA+T TVHVGDVTDL+V +L G APVFT+G AP PV+C VQV Sbjct 241 GIPGPGPDGRPRYVTGIDAETGTVHVGDVTDLEVTSLLGEAPVFTSGVAPDAPVECAVQV 300 Query 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGA 356 RAHG V AVAEL AL V L PLRGVA GQTLVLYR DP GDEVLGSATI+ A Sbjct 301 RAHGGVVDAVAELRDGALEVSLRTPLRGVAPGQTLVLYRSDPDGDEVLGSATISAA 356 >gi|145224833|ref|YP_001135511.1| tRNA-specific 2-thiouridylase MnmA [Mycobacterium gilvum PYR-GCK] gi|315445164|ref|YP_004078043.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Mycobacterium sp. Spyr1] gi|189040505|sp|A4TDZ4.1|MNMA_MYCGI RecName: Full=tRNA-specific 2-thiouridylase mnmA gi|145217319|gb|ABP46723.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Mycobacterium gilvum PYR-GCK] gi|315263467|gb|ADU00209.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Mycobacterium sp. Spyr1] Length=357 Score = 578 bits (1489), Expect = 7e-163, Method: Compositional matrix adjust. Identities = 287/353 (82%), Positives = 313/353 (89%), Gaps = 0/353 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 M+VL AMSGGVDSSVAAARMV AGH+VVGVH+ALS+APGTLRTGSRGCCSKEDA DARRV Sbjct 1 MRVLVAMSGGVDSSVAAARMVAAGHDVVGVHLALSSAPGTLRTGSRGCCSKEDAGDARRV 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVLGIPFYVWDFA++FKEDVI+DFV SYARGETPNPCVRCN++IKF+AL++RA+ALGFD Sbjct 61 ADVLGIPFYVWDFADRFKEDVIDDFVESYARGETPNPCVRCNERIKFSALASRALALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 +ATGHYARL+ GRLRRAVD DKDQSYVLAVLTA+QLRHA FPIGDTPK QIR EAAR G Sbjct 121 ALATGHYARLADGRLRRAVDHDKDQSYVLAVLTAEQLRHAVFPIGDTPKAQIREEAARLG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 LAVA+K DSHDICFIPSG+TKAFLG RIGVRRG VVDA G VLA HDGVHGFTIGQR+GL Sbjct 181 LAVADKADSHDICFIPSGDTKAFLGARIGVRRGNVVDAAGTVLAEHDGVHGFTIGQRKGL 240 Query 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 GI GPGP+G PRYVT IDA T TVHVGDV DL+V +L G APVFT+G AP+GP++CVVQV Sbjct 241 GIPGPGPDGSPRYVTGIDAQTGTVHVGDVADLEVTSLQGDAPVFTSGVAPTGPLECVVQV 300 Query 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATI 353 RAHG AVAEL GD L V L PLRGVA GQTLVLYR DPAGDEVL SATI Sbjct 301 RAHGGVADAVAELRGDTLEVSLRTPLRGVAPGQTLVLYRRDPAGDEVLASATI 353 >gi|333991381|ref|YP_004523995.1| tRNA (5-methylaminomethyl-2-thiouridylate)- methyltransferase TrmU [Mycobacterium sp. JDM601] gi|333487349|gb|AEF36741.1| tRNA (5-methylaminomethyl-2-thiouridylate)- methyltransferase TrmU [Mycobacterium sp. JDM601] Length=358 Score = 577 bits (1488), Expect = 9e-163, Method: Compositional matrix adjust. Identities = 286/355 (81%), Positives = 317/355 (90%), Gaps = 0/355 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 MKV+AAMSGGVDSSVAAARMVDAGH+VVGVH+ALS PGTLRTGSRGCCS+ED+ DARRV Sbjct 1 MKVVAAMSGGVDSSVAAARMVDAGHDVVGVHLALSRNPGTLRTGSRGCCSREDSDDARRV 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVLGIPFYVWDF+E+F+E+VI+DFV SYARGETPNPCVRCN++IKF+A++ARA+ALGFD Sbjct 61 ADVLGIPFYVWDFSEQFQEEVIDDFVESYARGETPNPCVRCNEKIKFSAVAARALALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 VATGHYARL+ GRLRRAVD DKDQSYVLAVLTA+QL HAAFP+GDTPK IRAEAA RG Sbjct 121 MVATGHYARLADGRLRRAVDADKDQSYVLAVLTAEQLSHAAFPVGDTPKPAIRAEAAERG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 LAVA+KPDSHDICFIP+G+T+ FLG IGVR G VVDA G VLA HDGVHGFTIGQR+GL Sbjct 181 LAVASKPDSHDICFIPTGDTRTFLGSHIGVRPGAVVDAGGEVLAEHDGVHGFTIGQRKGL 240 Query 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 GIAGPGP+GRPRYVT IDA TATV VG V DLDV LTGRAPVFT+G A GPV+C VQV Sbjct 241 GIAGPGPDGRPRYVTGIDAATATVTVGTVADLDVLELTGRAPVFTSGGAWHGPVECEVQV 300 Query 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAG 355 RAHGETV AVAEL+G+ L V+L PLRGVARGQTL YRPDPAGDEV+ SATIAG Sbjct 301 RAHGETVGAVAELVGEELQVRLREPLRGVARGQTLACYRPDPAGDEVIASATIAG 355 >gi|126434444|ref|YP_001070135.1| tRNA-specific 2-thiouridylase MnmA [Mycobacterium sp. JLS] gi|166235066|sp|A3PXL7.1|MNMA_MYCSJ RecName: Full=tRNA-specific 2-thiouridylase mnmA gi|126234244|gb|ABN97644.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Mycobacterium sp. JLS] Length=359 Score = 568 bits (1463), Expect = 6e-160, Method: Compositional matrix adjust. Identities = 283/358 (80%), Positives = 310/358 (87%), Gaps = 0/358 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 M+VL AMSGGVDSSVAAARMVDAGH+VVGVH+ALS PGTLRTGSRGCCSKEDA DARRV Sbjct 1 MRVLVAMSGGVDSSVAAARMVDAGHDVVGVHLALSATPGTLRTGSRGCCSKEDAGDARRV 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVL IPFYVWDFA++FKEDVI+DFV+SY RGETPNPCVRCN++IKF+AL+ RA+ALGFD Sbjct 61 ADVLDIPFYVWDFADRFKEDVIDDFVASYERGETPNPCVRCNEKIKFSALAGRALALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 +ATGHYARLS GRLRRAVD DKDQSYVLAVLTAQQLRHA FPIGDTPK QIRAEAA RG Sbjct 121 ALATGHYARLSDGRLRRAVDADKDQSYVLAVLTAQQLRHAVFPIGDTPKAQIRAEAAERG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 LAVA+KPDSHDICFIPSG+T+AFLG RIGVRRG VVDA G LA H+GVHGFTIGQR+GL Sbjct 181 LAVADKPDSHDICFIPSGDTRAFLGARIGVRRGAVVDAGGTKLAEHEGVHGFTIGQRKGL 240 Query 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 GIAGPGP+G+PRYVT IDA T TV VG DLDV LTG PVFT+GAA GPV+C VQV Sbjct 241 GIAGPGPDGQPRYVTGIDAATGTVRVGGAEDLDVSRLTGERPVFTSGAAFGGPVECQVQV 300 Query 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGASG 358 RAHG AVA L V+L APLRGVA GQT+V+YRPDP GDEVL SATI+GA+G Sbjct 301 RAHGGLADAVASHDAGVLSVELRAPLRGVAAGQTMVIYRPDPEGDEVLASATISGAAG 358 >gi|108798844|ref|YP_639041.1| tRNA-specific 2-thiouridylase MnmA [Mycobacterium sp. MCS] gi|119867959|ref|YP_937911.1| tRNA-specific 2-thiouridylase MnmA [Mycobacterium sp. KMS] gi|123179026|sp|Q1BAU9.1|MNMA_MYCSS RecName: Full=tRNA-specific 2-thiouridylase mnmA gi|166235067|sp|A1UE63.1|MNMA_MYCSK RecName: Full=tRNA-specific 2-thiouridylase mnmA gi|108769263|gb|ABG07985.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Mycobacterium sp. MCS] gi|119694048|gb|ABL91121.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Mycobacterium sp. KMS] Length=359 Score = 566 bits (1460), Expect = 1e-159, Method: Compositional matrix adjust. Identities = 283/358 (80%), Positives = 309/358 (87%), Gaps = 0/358 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 M+VL AMSGGVDSSVAAARMVDAGH+VVGVH+ALS PGTLRTGSRGCCSKEDA DARRV Sbjct 1 MRVLVAMSGGVDSSVAAARMVDAGHDVVGVHLALSATPGTLRTGSRGCCSKEDAGDARRV 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVL IPFYVWDFA++FKEDVI+DFV+SY RGETPNPCVRCN++IKF+AL+ RA+ALGFD Sbjct 61 ADVLDIPFYVWDFADRFKEDVIDDFVASYERGETPNPCVRCNEKIKFSALAGRALALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 +ATGHYARLS GRLRRAVD DKDQSYVLAVLTAQQLRHA FPIGDTPK QIRAEAA RG Sbjct 121 ALATGHYARLSDGRLRRAVDADKDQSYVLAVLTAQQLRHAVFPIGDTPKAQIRAEAAERG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 LAVA+KPDSHDICFIPSG+T+AFLG RIGVRRG VVDA G LA H+GVHGFTIGQR+GL Sbjct 181 LAVADKPDSHDICFIPSGDTRAFLGARIGVRRGAVVDAGGTKLAEHEGVHGFTIGQRKGL 240 Query 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 GIAGPGP+G+PRYVT IDA T TV VG DLDV LTG PVFT+GAA GPV+C VQV Sbjct 241 GIAGPGPDGQPRYVTGIDAATGTVRVGGAEDLDVWRLTGERPVFTSGAAFGGPVECQVQV 300 Query 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGASG 358 RAHG AVA L V+L APLRGVA GQT+V+YRPDP GDEVL SATI+GA G Sbjct 301 RAHGGLADAVASHDAGVLSVELRAPLRGVAAGQTMVIYRPDPEGDEVLASATISGADG 358 >gi|118473593|ref|YP_886701.1| tRNA-specific 2-thiouridylase MnmA [Mycobacterium smegmatis str. MC2 155] gi|166235065|sp|A0QUW3.1|MNMA_MYCS2 RecName: Full=tRNA-specific 2-thiouridylase mnmA gi|118174880|gb|ABK75776.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Mycobacterium smegmatis str. MC2 155] Length=356 Score = 551 bits (1419), Expect = 8e-155, Method: Compositional matrix adjust. Identities = 279/353 (80%), Positives = 307/353 (87%), Gaps = 0/353 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 M+VL AMSGGVDSSVAAARMVDAGH+VVGVH+ALS+APGTLRTGSRGCCSKEDA+DARRV Sbjct 1 MRVLVAMSGGVDSSVAAARMVDAGHDVVGVHLALSSAPGTLRTGSRGCCSKEDASDARRV 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 AD+LGIPFYVWDFA++FK+DVI+DFV SYARGETPNPCVRCN++IKF+AL+ARA+ALGFD Sbjct 61 ADILGIPFYVWDFADRFKDDVIDDFVESYARGETPNPCVRCNERIKFSALAARALALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 VATGHYARLS GRLRRAVD DKDQSYVL VLTA+QLRHA FPIGDTPK QIRAEAA RG Sbjct 121 AVATGHYARLSDGRLRRAVDADKDQSYVLGVLTAEQLRHALFPIGDTPKPQIRAEAAERG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 LAVA KPDSHDICFIP+G+TKAFLG RIGVR G VVD G VLA HDGVHGFTIGQR+GL Sbjct 181 LAVAEKPDSHDICFIPTGDTKAFLGARIGVRPGAVVDGSGTVLAEHDGVHGFTIGQRKGL 240 Query 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 GIAGPG +GRPRYVTAIDA+T TV VG DL+V LTG PVFT+G P GP++C VQV Sbjct 241 GIAGPGADGRPRYVTAIDAETGTVRVGPAEDLEVWELTGERPVFTSGVEPGGPIECQVQV 300 Query 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATI 353 RAHG VAEL L + L APLRGVA GQT+VLYRPDP GDEV+ SATI Sbjct 301 RAHGGITDTVAELRDGKLELSLRAPLRGVAPGQTMVLYRPDPDGDEVIASATI 353 >gi|169630437|ref|YP_001704086.1| tRNA-specific 2-thiouridylase MnmA [Mycobacterium abscessus ATCC 19977] gi|229486416|sp|B1MDW3.1|MNMA_MYCA9 RecName: Full=tRNA-specific 2-thiouridylase mnmA gi|169242404|emb|CAM63432.1| Probable tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Mycobacterium abscessus] Length=356 Score = 533 bits (1372), Expect = 2e-149, Method: Compositional matrix adjust. Identities = 263/353 (75%), Positives = 297/353 (85%), Gaps = 0/353 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 MK+LAAMSGGVDSSVAAARMVDAGHEVVGVH+ALS APGTLRTGSRGCCSKEDAADARRV Sbjct 1 MKILAAMSGGVDSSVAAARMVDAGHEVVGVHLALSAAPGTLRTGSRGCCSKEDAADARRV 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 AD+LGIPFYVWDFA++FKEDVI+DFV +YA G TPNPCV+CN++IKFAAL+ RAVALGFD Sbjct 61 ADMLGIPFYVWDFADRFKEDVIDDFVEAYAEGRTPNPCVKCNEKIKFAALADRAVALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 VATGHYARL GRLRRAVD DKDQSYVL VLT +QLR A FP+GD+PK IRAEA +RG Sbjct 121 AVATGHYARLQDGRLRRAVDADKDQSYVLGVLTPEQLRRAMFPVGDSPKPDIRAEAEQRG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 L VANKPDSHDICFIPSG+T+AFLG RIGVRRG VVDADG VLA+H GVH FTIGQR+GL Sbjct 181 LLVANKPDSHDICFIPSGDTQAFLGARIGVRRGNVVDADGSVLATHAGVHEFTIGQRKGL 240 Query 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 G+ GP +GRPRYVT+IDA+TATV VG V DL++ G V+T+G P P++C VQV Sbjct 241 GLVGPAADGRPRYVTSIDAETATVRVGTVEDLEIWEFGGEPVVWTSGRVPGQPIECQVQV 300 Query 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATI 353 RAHG V A+ E + + V+L LRGVA GQT+VLYRPD GDEVLGSA I Sbjct 301 RAHGSVVDAIVEPGAERIHVRLRTALRGVAPGQTVVLYRPDAEGDEVLGSAVI 353 >gi|54026238|ref|YP_120480.1| tRNA-specific 2-thiouridylase MnmA [Nocardia farcinica IFM 10152] gi|81602523|sp|Q5YRS5.1|MNMA_NOCFA RecName: Full=tRNA-specific 2-thiouridylase mnmA gi|54017746|dbj|BAD59116.1| putative tRNA methyl transferase [Nocardia farcinica IFM 10152] Length=372 Score = 511 bits (1317), Expect = 6e-143, Method: Compositional matrix adjust. Identities = 262/357 (74%), Positives = 298/357 (84%), Gaps = 0/357 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 M+VLAAMSGGVDS+VAAAR VDAGH+VVGVH+ALS PGTLRTGSRGCCSKEDA DARR Sbjct 2 MRVLAAMSGGVDSAVAAARAVDAGHDVVGVHLALSATPGTLRTGSRGCCSKEDAGDARRA 61 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVLGIPFYVWDFA++FKEDVI+DFV++YA GETPNPC+RCN++IKF+AL+ RAVALGFD Sbjct 62 ADVLGIPFYVWDFADRFKEDVIDDFVAAYAAGETPNPCLRCNEKIKFSALADRAVALGFD 121 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 V TGHYARLS G LRRAVD DKDQSYVLAVLTA+QL A FP+GDTPK QIRAEAA RG Sbjct 122 AVVTGHYARLSDGVLRRAVDADKDQSYVLAVLTAEQLARAMFPVGDTPKPQIRAEAAERG 181 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 LAVANKPDSHDICFIPSG+T+AFLG RIG+R G VVDADG VLA H+GVHGFTIGQR+GL Sbjct 182 LAVANKPDSHDICFIPSGDTRAFLGARIGIRPGAVVDADGQVLAEHEGVHGFTIGQRKGL 241 Query 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 G+ GP +G+PRYVT ID + TV VG DL V+ + V+T+G P GP+DCV QV Sbjct 242 GLPGPAADGKPRYVTDIDPASGTVRVGSAEDLQVRQVRAERAVWTSGRTPEGPIDCVAQV 301 Query 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGAS 357 RAHG T A AE + L V+LH PL GVA+GQ +VLYRPDP GDEVLGS TI+G + Sbjct 302 RAHGGTAPATAEAVDGGLVVRLHEPLTGVAKGQAVVLYRPDPLGDEVLGSGTISGTA 358 >gi|325675868|ref|ZP_08155552.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Rhodococcus equi ATCC 33707] gi|325553839|gb|EGD23517.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Rhodococcus equi ATCC 33707] Length=372 Score = 511 bits (1315), Expect = 9e-143, Method: Compositional matrix adjust. Identities = 263/368 (72%), Positives = 299/368 (82%), Gaps = 11/368 (2%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 M+VLAAMSGGVDS+VAAAR V+AGH+VVGVH+ALSTAPGTLRTGSRGCCSKEDA DA+R Sbjct 1 MRVLAAMSGGVDSAVAAARAVEAGHDVVGVHLALSTAPGTLRTGSRGCCSKEDAGDAKRA 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVLGIPFYVWDFA++FKEDVI+DFV SYA GETPNPC+RCN++IKFAAL+ RA ALGFD Sbjct 61 ADVLGIPFYVWDFADRFKEDVIDDFVESYAAGETPNPCLRCNEKIKFAALADRAYALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 VATGHYARL G LRRAVD DKDQSYVLAVLTA QL A FP+GDTPK QIR EAA RG Sbjct 121 AVATGHYARLEDGELRRAVDADKDQSYVLAVLTADQLSRAMFPVGDTPKEQIREEAAARG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDAD-GVVLASHDGVHGFTIGQRRG 239 LAVANKPDSHDICFIPSG+T+AFLG RIG+R G VVDAD G VLA H+GVHGFTIGQR+G Sbjct 181 LAVANKPDSHDICFIPSGDTRAFLGARIGIRPGAVVDADTGEVLAQHEGVHGFTIGQRKG 240 Query 240 LGIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQ 299 LG+ GP +G+PRYVTAI+ +T TV VG LDV T+T ++T+G AP GP++C+VQ Sbjct 241 LGVQGPAADGQPRYVTAIEPETGTVRVGSAARLDVWTITAERAIWTSGQAPEGPIECIVQ 300 Query 300 VRAHGETVSAVAELI----------GDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLG 349 VRAHG T AVAE + + VQL PL GVA+GQ +VLYRPD GD V+G Sbjct 301 VRAHGGTARAVAEAVRGERSDGEGRDGGIVVQLSEPLTGVAKGQAVVLYRPDEDGDVVIG 360 Query 350 SATIAGAS 357 S TIAG S Sbjct 361 SGTIAGTS 368 >gi|226305865|ref|YP_002765825.1| tRNA uridine-sulfurtransferase [Rhodococcus erythropolis PR4] gi|229493398|ref|ZP_04387187.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Rhodococcus erythropolis SK121] gi|259495836|sp|C0ZXK1.1|MNMA_RHOE4 RecName: Full=tRNA-specific 2-thiouridylase mnmA gi|226184982|dbj|BAH33086.1| probable tRNA uridine-sulfurtransferase [Rhodococcus erythropolis PR4] gi|229319714|gb|EEN85546.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Rhodococcus erythropolis SK121] Length=370 Score = 506 bits (1304), Expect = 2e-141, Method: Compositional matrix adjust. Identities = 261/362 (73%), Positives = 298/362 (83%), Gaps = 7/362 (1%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 M+VLAAMSGGVDS+VAAAR V AGH+VVGVH+ALSTAPG LRTGSRGCCSKEDA DARR Sbjct 1 MRVLAAMSGGVDSAVAAARAVAAGHDVVGVHLALSTAPGALRTGSRGCCSKEDAGDARRA 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVLGIPFYVWDFA++FKEDVI+DFV+SYA GETPNPC+RCN++IKF AL+ RAVALGFD Sbjct 61 ADVLGIPFYVWDFADRFKEDVIDDFVASYAAGETPNPCLRCNEKIKFTALADRAVALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 VATGHYARL G LRRAVD DKDQSYVL VLTA QL A FPIGDTPK QIR EAA RG Sbjct 121 AVATGHYARLEDGVLRRAVDADKDQSYVLGVLTADQLSRAMFPIGDTPKEQIREEAAERG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDAD-GVVLASHDGVHGFTIGQRRG 239 LAVANKPDSHDICFIP+G+T+AFLG RIGVR G VVDAD G VLA+HDGVHGFTIGQR+G Sbjct 181 LAVANKPDSHDICFIPTGDTRAFLGARIGVRPGSVVDADSGEVLAAHDGVHGFTIGQRKG 240 Query 240 LGIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQ 299 LG+ GP +GRPRYVTAI+ +T TV VG +LDV ++ + ++T+G AP GPV+C+VQ Sbjct 241 LGVEGPAGDGRPRYVTAIEPETGTVRVGSAKNLDVWAISAQRAIWTSGQAPEGPVECMVQ 300 Query 300 VRAHGETVSAVAELIGD-----ALFVQLHAPLRGVARGQTLVLYRPDPA-GDEVLGSATI 353 VRAHG AVAE + D + + L PL GVA+GQ +VLYRPD GD+VLGS TI Sbjct 301 VRAHGGLAQAVAEAVDDGTSGGGISISLREPLTGVAKGQAVVLYRPDSELGDQVLGSGTI 360 Query 354 AG 355 +G Sbjct 361 SG 362 >gi|111023428|ref|YP_706400.1| tRNA-specific 2-thiouridylase MnmA [Rhodococcus jostii RHA1] gi|122955129|sp|Q0S2J4.1|MNMA_RHOSR RecName: Full=tRNA-specific 2-thiouridylase mnmA gi|110822958|gb|ABG98242.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Rhodococcus jostii RHA1] Length=364 Score = 504 bits (1299), Expect = 7e-141, Method: Compositional matrix adjust. Identities = 256/356 (72%), Positives = 295/356 (83%), Gaps = 1/356 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 M+VLAAMSGGVDS+VAAAR V AGH+VVGVH+ALS PGTLRTGSRGCCSKEDA DARR Sbjct 1 MRVLAAMSGGVDSAVAAARAVAAGHDVVGVHLALSAEPGTLRTGSRGCCSKEDAGDARRA 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVLGIPFYVWDFA++FKEDVI+DFV+SYA GETPNPC+RCN++IKFAAL+ RA+ALGFD Sbjct 61 ADVLGIPFYVWDFADRFKEDVIDDFVASYAAGETPNPCLRCNEKIKFAALADRAIALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 VATGHYA+L G LRRAVD DKDQSYVL VLTA+QL A FP+GDTPK +IR EAA RG Sbjct 121 AVATGHYAQLENGVLRRAVDADKDQSYVLGVLTAEQLSRAMFPVGDTPKDRIREEAAERG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDAD-GVVLASHDGVHGFTIGQRRG 239 LAVANKPDSHDICFIPSG+T+AFLG RIGVR G VVDAD G VLA H+GVHGFTIGQR+G Sbjct 181 LAVANKPDSHDICFIPSGDTRAFLGARIGVRPGSVVDADSGEVLAEHEGVHGFTIGQRKG 240 Query 240 LGIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQ 299 LG+ GP +G+PRYVT+I+ DT TV VG +L+V +T V+T+G+ P GPV+C VQ Sbjct 241 LGVQGPAADGQPRYVTSIEPDTGTVRVGSARNLEVDAITADRAVWTSGSTPVGPVECTVQ 300 Query 300 VRAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAG 355 VRAHG AVAE + + + L PL GVA+GQ +VLYR DPAGD VLGS TI+G Sbjct 301 VRAHGGLAEAVAEAVDGGIAISLREPLTGVAKGQAVVLYRTDPAGDIVLGSGTISG 356 >gi|226365929|ref|YP_002783712.1| tRNA-specific 2-thiouridylase MnmA [Rhodococcus opacus B4] gi|254811458|sp|C1B1S2.1|MNMA_RHOOB RecName: Full=tRNA-specific 2-thiouridylase mnmA gi|226244419|dbj|BAH54767.1| putative tRNA uridine-sulfurtransferase [Rhodococcus opacus B4] Length=364 Score = 504 bits (1297), Expect = 1e-140, Method: Compositional matrix adjust. Identities = 257/356 (73%), Positives = 294/356 (83%), Gaps = 1/356 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 M+VLAAMSGGVDS+VAAAR V AGH+VVGVH+ALS PGTLRTGSRGCCSKEDA DARR Sbjct 1 MRVLAAMSGGVDSAVAAARAVAAGHDVVGVHLALSAEPGTLRTGSRGCCSKEDAGDARRA 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVLGIPFYVWDFA++FKEDVI+DFV+SYA GETPNPC+RCN++IKFAAL+ RA+ALGFD Sbjct 61 ADVLGIPFYVWDFADRFKEDVIDDFVASYAAGETPNPCLRCNEKIKFAALADRAIALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 VATGHYA+L G LRRAVD DKDQSYVL VLTA+QL A FP+GDTPK +IR EAA RG Sbjct 121 AVATGHYAQLENGVLRRAVDADKDQSYVLGVLTAEQLSRAMFPVGDTPKDRIREEAAERG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDAD-GVVLASHDGVHGFTIGQRRG 239 LAVANKPDSHDICFIPSG+T+AFLG IGVR G VVDAD G VLA H+GVHGFTIGQR+G Sbjct 181 LAVANKPDSHDICFIPSGDTRAFLGAHIGVRPGSVVDADSGEVLAEHEGVHGFTIGQRKG 240 Query 240 LGIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQ 299 LG+ GP +G+PRYVT+I+ +T TV VG DL+V +T V+T+G APSGPV+C VQ Sbjct 241 LGVQGPAADGQPRYVTSIEPETGTVRVGSARDLEVHAITADRAVWTSGRAPSGPVECTVQ 300 Query 300 VRAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAG 355 VRAHG AVAE + + + L PL GVA+GQ VLYR DPAGD VLGS TI+G Sbjct 301 VRAHGGLAEAVAEAVDGGIRISLREPLTGVAKGQAAVLYRTDPAGDIVLGSGTISG 356 >gi|333920880|ref|YP_004494461.1| tRNA-specific 2-thiouridylase mnmA [Amycolicicoccus subflavus DQS3-9A1] gi|333483101|gb|AEF41661.1| tRNA-specific 2-thiouridylase mnmA [Amycolicicoccus subflavus DQS3-9A1] Length=375 Score = 499 bits (1285), Expect = 3e-139, Method: Compositional matrix adjust. Identities = 260/363 (72%), Positives = 302/363 (84%), Gaps = 1/363 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 M+VLAAMSGGVDS+VAAAR V AGH+VVGVH+ALSTAPG LRTGSRGCCS+EDA DARR Sbjct 1 MRVLAAMSGGVDSAVAAARAVAAGHDVVGVHLALSTAPGALRTGSRGCCSREDAGDARRA 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 AD+LGIPFYVWDFA+KFKEDVI+DFV+SYA GETPNPC+RCN++IKF+AL+ RA ALGFD Sbjct 61 ADILGIPFYVWDFADKFKEDVIDDFVASYAAGETPNPCLRCNEKIKFSALAERAYALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 V TGHYA+L G LRRAVD +KDQSYVLAVLTA+QL A FP+GDTPK +IR EAAR G Sbjct 121 AVVTGHYAQLRDGVLRRAVDAEKDQSYVLAVLTAEQLARAMFPVGDTPKPEIRNEAARLG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDAD-GVVLASHDGVHGFTIGQRRG 239 L+VANKPDSHDICFIP+GNT+AFLG RIGVR G VVD++ G VLA H+GVHGFT+GQR+G Sbjct 181 LSVANKPDSHDICFIPTGNTQAFLGARIGVRPGKVVDSESGAVLAEHEGVHGFTVGQRKG 240 Query 240 LGIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQ 299 LG+ GP +G+PRYVT ID +T+TV VG DL+V T+T R ++T+GAAP G VDCVVQ Sbjct 241 LGLPGPAADGKPRYVTGIDPETSTVTVGSAADLEVWTITARDVIWTSGAAPEGAVDCVVQ 300 Query 300 VRAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGASGL 359 VRAHG T +A AE G + + L +PL GVARGQ VLYR DPAGD VLGS TIAG S Sbjct 301 VRAHGGTAAATAEPEGSGMRITLASPLTGVARGQAAVLYREDPAGDIVLGSGTIAGTSAQ 360 Query 360 STG 362 +G Sbjct 361 RSG 363 >gi|312140513|ref|YP_004007849.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase trmu [Rhodococcus equi 103S] gi|311889852|emb|CBH49169.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase TrmU [Rhodococcus equi 103S] Length=362 Score = 492 bits (1267), Expect = 4e-137, Method: Compositional matrix adjust. Identities = 253/358 (71%), Positives = 289/358 (81%), Gaps = 11/358 (3%) Query 11 VDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRVADVLGIPFYV 70 +DS+VAAAR V+AGH+VVGVH+ALSTAPGTLRTGSRGCCSKEDA DA+R ADVLGIPFYV Sbjct 1 MDSAVAAARAVEAGHDVVGVHLALSTAPGTLRTGSRGCCSKEDAGDAKRAADVLGIPFYV 60 Query 71 WDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFDTVATGHYARL 130 WDFA++FKEDVI+DFV SYA GETPNPC+RCN++IKFAAL+ RA ALGFD VATGHYARL Sbjct 61 WDFADRFKEDVIDDFVESYAAGETPNPCLRCNEKIKFAALADRAYALGFDAVATGHYARL 120 Query 131 SGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRGLAVANKPDSH 190 G LRRAVD DKDQSYVLAVLTA QL A FP+GDTPK QIR EAA RGLAVANKPDSH Sbjct 121 EDGELRRAVDADKDQSYVLAVLTADQLSRAMFPVGDTPKEQIREEAAARGLAVANKPDSH 180 Query 191 DICFIPSGNTKAFLGERIGVRRGVVVDAD-GVVLASHDGVHGFTIGQRRGLGIAGPGPNG 249 DICFIPSG+T+AFLG RIG+R G VVDAD G VLA H+GVHGFTIGQR+GLG+ GP +G Sbjct 181 DICFIPSGDTRAFLGARIGIRPGAVVDADTGEVLAQHEGVHGFTIGQRKGLGVQGPAADG 240 Query 250 RPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQVRAHGETVSA 309 +PRYVTAI+ +T TV VG LDV T+T ++T+G AP GP++C+VQVRAHG T A Sbjct 241 QPRYVTAIEPETGTVRVGSAARLDVWTITAERAIWTSGQAPEGPIECIVQVRAHGGTARA 300 Query 310 VAELI----------GDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGAS 357 VAE + + VQL PL GVA+GQ +VLYRPD GD V+GS TIAG S Sbjct 301 VAEAVRGERSDGEGRDGGIVVQLSEPLTGVAKGQAVVLYRPDEDGDVVIGSGTIAGTS 358 >gi|298526491|ref|ZP_07013900.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Mycobacterium tuberculosis 94_M4241A] gi|298496285|gb|EFI31579.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Mycobacterium tuberculosis 94_M4241A] Length=286 Score = 483 bits (1243), Expect = 2e-134, Method: Compositional matrix adjust. Identities = 264/289 (92%), Positives = 267/289 (93%), Gaps = 6/289 (2%) Query 82 INDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFDTVATGHYARLSG---GRLRRA 138 +NDFVSSYA G PCVRCNQQIKFAALS + +GF GH A G GRLRRA Sbjct 1 MNDFVSSYAAGGNSEPCVRCNQQIKFAALS-QGCGVGFRY--GGHRATTPGCRVGRLRRA 57 Query 139 VDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRGLAVANKPDSHDICFIPSG 198 VDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRGLAVANKPDSHDICFIPSG Sbjct 58 VDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRGLAVANKPDSHDICFIPSG 117 Query 199 NTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGLGIAGPGPNGRPRYVTAID 258 NTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGLGIAGPGPNGRPRYVTAID Sbjct 118 NTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGLGIAGPGPNGRPRYVTAID 177 Query 259 ADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQVRAHGETVSAVAELIGDAL 318 ADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQVRAHGETVSAVAELIGDAL Sbjct 178 ADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQVRAHGETVSAVAELIGDAL 237 Query 319 FVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGASGLSTGGNPGA 367 FVQLHAPL GVARGQTLVLYRPDPAGDEVLGSATIAGASGLSTGGNPGA Sbjct 238 FVQLHAPLCGVARGQTLVLYRPDPAGDEVLGSATIAGASGLSTGGNPGA 286 >gi|289575733|ref|ZP_06455960.1| LOW QUALITY PROTEIN: tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase trmU [Mycobacterium tuberculosis K85] gi|289540164|gb|EFD44742.1| LOW QUALITY PROTEIN: tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase trmU [Mycobacterium tuberculosis K85] Length=367 Score = 479 bits (1232), Expect = 4e-133, Method: Compositional matrix adjust. Identities = 243/244 (99%), Positives = 244/244 (100%), Gaps = 0/244 (0%) Query 124 TGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRGLAV 183 TGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRGLAV Sbjct 124 TGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRGLAV 183 Query 184 ANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGLGIA 243 ANKPDSHDICFIPSGNT+AFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGLGIA Sbjct 184 ANKPDSHDICFIPSGNTRAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGLGIA 243 Query 244 GPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQVRAH 303 GPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQVRAH Sbjct 244 GPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQVRAH 303 Query 304 GETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGASGLSTGG 363 GETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGASGLSTGG Sbjct 304 GETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGASGLSTGG 363 Query 364 NPGA 367 NPGA Sbjct 364 NPGA 367 >gi|296140603|ref|YP_003647846.1| tRNA(5-methylaminomethyl-2-thiouridylate)-methyl transferase [Tsukamurella paurometabola DSM 20162] gi|296028737|gb|ADG79507.1| tRNA(5-methylaminomethyl-2-thiouridylate)-methyl transferase [Tsukamurella paurometabola DSM 20162] Length=365 Score = 471 bits (1211), Expect = 1e-130, Method: Compositional matrix adjust. Identities = 248/355 (70%), Positives = 284/355 (80%), Gaps = 2/355 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 M+VLAAMSGGVDS+VAAAR V+AGH+VVGVH+ALS APGTLRTGSRGCCSKEDA DARR Sbjct 1 MRVLAAMSGGVDSAVAAARAVEAGHDVVGVHLALSEAPGTLRTGSRGCCSKEDAGDARRA 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVLGIPFYVWDFA++F+EDVI+DFV++YA GETPNPC+RCN++IKF+AL+ RA ALGFD Sbjct 61 ADVLGIPFYVWDFADRFREDVIDDFVAAYAAGETPNPCLRCNEKIKFSALAERAYALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 VATGHYARL GG LRRAVD DKDQSYVLAVLTA+QL A FP+GDTPK IR EAARRG Sbjct 121 AVATGHYARLQGGELRRAVDPDKDQSYVLAVLTAEQLSRAMFPVGDTPKPLIREEAARRG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVD-ADGVVLASHDGVHGFTIGQRRG 239 LAVA+KPDSHDICFIPSG+T+AFLG RIGVR G VVD A G L H GVH FTIGQR+G Sbjct 181 LAVADKPDSHDICFIPSGDTQAFLGARIGVRPGAVVDAATGERLGEHGGVHEFTIGQRKG 240 Query 240 LGIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQ 299 LG+ GPGP+G PRYVTAIDA + V VG LDV + V+T+G+ P+ P++ +VQ Sbjct 241 LGLPGPGPDGAPRYVTAIDAASGEVRVGRGEQLDVTRIVADGAVWTSGSVPTQPIEAMVQ 300 Query 300 VRAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDP-AGDEVLGSATI 353 VRAHG A GD + + L PLRGVA GQ VLY PD GD V+GS TI Sbjct 301 VRAHGGLAPVTARAEGDRIVMDLAEPLRGVAPGQAAVLYAPDQDRGDLVVGSGTI 355 >gi|262203144|ref|YP_003274352.1| tRNA(5-methylaminomethyl-2-thiouridylate)-methyl transferase [Gordonia bronchialis DSM 43247] gi|262086491|gb|ACY22459.1| tRNA(5-methylaminomethyl-2-thiouridylate)-methyl transferase [Gordonia bronchialis DSM 43247] Length=373 Score = 470 bits (1210), Expect = 1e-130, Method: Compositional matrix adjust. Identities = 247/363 (69%), Positives = 292/363 (81%), Gaps = 6/363 (1%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 M+VLAAMSGGVDS+VAAAR V+AGH+VVGVH+ALSTAPG LRTGSRGCCS+EDA+DARR Sbjct 1 MRVLAAMSGGVDSAVAAARAVEAGHDVVGVHLALSTAPGALRTGSRGCCSREDASDARRA 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVLGIPFYVWDFA++FK+DVI++FV++YA G+TPNPC+ CN+++KFAAL+ +AVALGFD Sbjct 61 ADVLGIPFYVWDFADRFKDDVIDEFVAAYAAGQTPNPCLTCNEKVKFAALAEKAVALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 +ATGHYARL+ G LRRAVD DKDQSYVLAVLT +QL A FP+GDTPK +IRAEAARRG Sbjct 121 ALATGHYARLADGELRRAVDADKDQSYVLAVLTRKQLGRAMFPVGDTPKPEIRAEAARRG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVD-ADGVVLASHDGVHGFTIGQRRG 239 L+VANKPDSHDICFIPSG+T+AFLG RIG+R G VVD A G LA H+GVHGFTIGQR+G Sbjct 181 LSVANKPDSHDICFIPSGDTRAFLGARIGLRPGAVVDAATGDRLADHEGVHGFTIGQRKG 240 Query 240 LGIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQ 299 LGI P +G+PRYVT ID D+ TV VG DL+V +++ R V+T+G P GPVDCVVQ Sbjct 241 LGIDAPAADGKPRYVTDIDPDSGTVTVGTAADLEVWSISARRAVWTSGHTPDGPVDCVVQ 300 Query 300 VRAHGETVSAVAELIG----DALFVQLHAPLRGVARGQTLVLYRPDPA-GDEVLGSATIA 354 VRAHG A A + + ++L PL GVARGQ VLY PD GD VLG IA Sbjct 301 VRAHGGLAPARATPVDRDGEPGIEIELTEPLTGVARGQAAVLYLPDAQRGDLVLGCGRIA 360 Query 355 GAS 357 S Sbjct 361 ATS 363 >gi|326381518|ref|ZP_08203212.1| tRNA-specific 2-thiouridylase MnmA [Gordonia neofelifaecis NRRL B-59395] gi|326199765|gb|EGD56945.1| tRNA-specific 2-thiouridylase MnmA [Gordonia neofelifaecis NRRL B-59395] Length=362 Score = 463 bits (1192), Expect = 2e-128, Method: Compositional matrix adjust. Identities = 248/362 (69%), Positives = 289/362 (80%), Gaps = 5/362 (1%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 M+VLAAMSGGVDS+VAAAR V+AGHEVVGVH+ALSTAPG LRTGSRGCCS+EDA DARR Sbjct 1 MRVLAAMSGGVDSAVAAARAVEAGHEVVGVHLALSTAPGALRTGSRGCCSREDADDARRA 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 AD+LGIPFYVWDFA++FKEDVI++FV++YA GETPNPC+ CN++IKF+AL+ RAVALGFD Sbjct 61 ADLLGIPFYVWDFADRFKEDVIDEFVAAYAAGETPNPCLSCNEKIKFSALAERAVALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 + TGHYARL+ G LRRAVD DKDQSYVLAVL QQL A FP+GDTPK +IR EA RRG Sbjct 121 ALVTGHYARLADGELRRAVDADKDQSYVLAVLDHQQLSRAMFPVGDTPKPRIREEAERRG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVD-ADGVVLASHDGVHGFTIGQRRG 239 LAVANKPDSHDICFIP+G+T+AFLG RIGVR G VVD A G LA HDGVHGFTIGQR+G Sbjct 181 LAVANKPDSHDICFIPTGDTRAFLGARIGVRPGAVVDGATGDTLAEHDGVHGFTIGQRKG 240 Query 240 LGIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQ 299 LGI P +G PRYVT ID D+ TV VG DL+V +++ V+T+G P GPV+C+VQ Sbjct 241 LGIEAPAADGHPRYVTGIDPDSGTVTVGTAADLEVTSISANRAVWTSGRTPEGPVECIVQ 300 Query 300 VRAHGETVSAVAELI----GDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAG 355 VRAHG AVA + G + + L PLRGVARGQ VLY PD AGD+V+G I G Sbjct 301 VRAHGGLAQAVATPVDTPDGPGIQIDLREPLRGVARGQAAVLYVPDAAGDQVIGCGRITG 360 Query 356 AS 357 + Sbjct 361 SE 362 >gi|343926874|ref|ZP_08766367.1| tRNA-specific 2-thiouridylase MnmA [Gordonia alkanivorans NBRC 16433] gi|343763234|dbj|GAA13293.1| tRNA-specific 2-thiouridylase MnmA [Gordonia alkanivorans NBRC 16433] Length=372 Score = 450 bits (1158), Expect = 1e-124, Method: Compositional matrix adjust. Identities = 245/367 (67%), Positives = 285/367 (78%), Gaps = 6/367 (1%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 M+VLAAMSGGVDS+VAAAR VDAGHEVVGVH+ALSTAPG LRTGSRGCCS+EDA+DARR Sbjct 1 MRVLAAMSGGVDSAVAAARAVDAGHEVVGVHLALSTAPGALRTGSRGCCSREDASDARRA 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVLGIPFYVWDFA++FK+DVI++FV +YA G TPNPC+ CN++IKFAAL+ +A+ALGFD Sbjct 61 ADVLGIPFYVWDFADRFKDDVIDEFVEAYAAGRTPNPCLTCNEKIKFAALADKAIALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 +ATGHYA+L G LRRAVD DKDQSYVLAVLT +QL A FPIGDTPK +IRAEAARRG Sbjct 121 ALATGHYAQLVDGELRRAVDHDKDQSYVLAVLTREQLSRALFPIGDTPKAEIRAEAARRG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDA-DGVVLASHDGVHGFTIGQRRG 239 LAVA+KPDSHDICFIPSG+T+AFLG +IG+R G VVDA G LA H+GVHGFTIGQR+G Sbjct 181 LAVADKPDSHDICFIPSGDTRAFLGAKIGIRPGAVVDASSGERLADHEGVHGFTIGQRKG 240 Query 240 LGIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQ 299 LGI P +G+PRYVT ID + TV VG DL V +T V+T+G P P VVQ Sbjct 241 LGIDAPAADGKPRYVTEIDPASGTVTVGTAEDLQVWGITATRAVWTSGITPDEPFVAVVQ 300 Query 300 VRAHG--ETVSAVAELIG--DALFVQLHAPLRGVARGQTLVLYRPDPA-GDEVLGSATIA 354 VRAHG V+A + A+ + LH PL GVARGQ VLY PD GD+V+G IA Sbjct 301 VRAHGGLAPVTATPTTVAGEPAIDIALHEPLTGVARGQAAVLYLPDAEHGDQVVGCGRIA 360 Query 355 GASGLST 361 S T Sbjct 361 STSSQPT 367 >gi|237785842|ref|YP_002906547.1| tRNA-specific 2-thiouridylase MnmA [Corynebacterium kroppenstedtii DSM 44385] gi|259495826|sp|C4LJJ9.1|MNMA_CORK4 RecName: Full=tRNA-specific 2-thiouridylase mnmA gi|237758754|gb|ACR18004.1| tRNA (5-methylaminomethyl-2-thiouridylate)- methyltransferase [Corynebacterium kroppenstedtii DSM 44385] Length=372 Score = 449 bits (1155), Expect = 3e-124, Method: Compositional matrix adjust. Identities = 223/360 (62%), Positives = 263/360 (74%), Gaps = 1/360 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 M+V+AAMSGGVDSSVAAAR+V GHEV+GVH+AL++ P TLR GSRGCCS ED+ DARRV Sbjct 1 MRVMAAMSGGVDSSVAAARLVAEGHEVIGVHLALASTPTTLRVGSRGCCSLEDSGDARRV 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 AD LGIPFYVWDF+++F+EDV++ F++SY GETPNPC+RCN++IKF AL R +ALGFD Sbjct 61 ADALGIPFYVWDFSDRFREDVVDPFINSYEMGETPNPCLRCNEKIKFQALLDRGIALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 VATGHYARL G LRRA+D +KDQSYVL VL +QL H FP+GDT K IR EA G Sbjct 121 AVATGHYARLKDGELRRAIDDNKDQSYVLGVLNDEQLAHCLFPLGDTRKPDIRKEAEAAG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 L A+KPDSHDICFIP GNTKAFLG IGVR G + D DG V+ HDG++GFTIGQR+GL Sbjct 181 LVTASKPDSHDICFIPDGNTKAFLGNHIGVRSGDLKDQDGNVIGHHDGIYGFTIGQRKGL 240 Query 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 G+ GP P+G+PRYVT IDADT TV VG DL V L + +C VQV Sbjct 241 GLPGPAPDGKPRYVTDIDADTGTVTVGTRDDLRVGRLYADR-LIRLDEHEGSTQECQVQV 299 Query 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGASGLS 360 RAHG V A + + LH+PL GVARGQ V+YRPDP GD VLGS TI +S Sbjct 300 RAHGGVVDATVTIDDGVATIDLHSPLTGVARGQAAVIYRPDPDGDIVLGSGTICDTVSVS 359 >gi|340794771|ref|YP_004760234.1| tRNA 5-methylaminomethyl-2-thiouridylate-methyltransferase [Corynebacterium variabile DSM 44702] gi|340534681|gb|AEK37161.1| tRNA 5-methylaminomethyl-2-thiouridylate-methyltransferase [Corynebacterium variabile DSM 44702] Length=380 Score = 436 bits (1121), Expect = 3e-120, Method: Compositional matrix adjust. Identities = 216/354 (62%), Positives = 264/354 (75%), Gaps = 0/354 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 M+V+AAMSGGVDSSVAAAR + GHEV+GVH+ALS +P +R GSRGCCS ED+ADARRV Sbjct 11 MRVVAAMSGGVDSSVAAARALAEGHEVIGVHLALSQSPEAVRAGSRGCCSLEDSADARRV 70 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 D LGIPFYVWDF+++FK DVI++FV SY GETPNPC+RCN++IKF AL R++ALGFD Sbjct 71 CDSLGIPFYVWDFSDRFKADVIDNFVESYEIGETPNPCLRCNEKIKFEALLDRSIALGFD 130 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 V TGHYA+L G LRR +D +KDQSYVL VLT +QL H FP+GDT K IRAEA G Sbjct 131 AVVTGHYAQLHDGVLRRGIDDNKDQSYVLGVLTDEQLAHCMFPVGDTVKPAIRAEAKDLG 190 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 + VA+KPDSHDICFIP G T+AFLG +IG+R G+V +G LA HDGV+GFTIGQR+GL Sbjct 191 MGVASKPDSHDICFIPDGRTQAFLGTKIGMRPGIVQSVEGETLAEHDGVYGFTIGQRKGL 250 Query 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 G+ GP +G+PRYVT I+A T TV VG DL + +T A ++C VQV Sbjct 251 GLPGPAADGQPRYVTDINASTGTVTVGRKRDLRIGHITADRLKRLDPAKHGETLECEVQV 310 Query 301 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIA 354 RAHG V A A L DA+ ++LH PL+GVARGQ V+Y PD GD ++GS TI+ Sbjct 311 RAHGGVVPATATLTSDAVELELHEPLQGVARGQAAVVYLPDAEGDILIGSGTIS 364 >gi|68536405|ref|YP_251110.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Corynebacterium jeikeium K411] gi|123650729|sp|Q4JUL5.1|MNMA_CORJK RecName: Full=tRNA-specific 2-thiouridylase mnmA gi|68264004|emb|CAI37492.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Corynebacterium jeikeium K411] Length=395 Score = 431 bits (1107), Expect = 1e-118, Method: Compositional matrix adjust. Identities = 222/384 (58%), Positives = 270/384 (71%), Gaps = 18/384 (4%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 M+V+AAMSGGVDS+VAA+R ++AGHEV+GVH+ALS +P +R GSRGCCS ED+ADARRV Sbjct 1 MRVVAAMSGGVDSAVAASRALEAGHEVIGVHLALSQSPEAVRAGSRGCCSLEDSADARRV 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 AD LGIPFYVWDF+++FK DVI+DFV SYA GETPNPC+RCN++IKF AL R++ALGFD Sbjct 61 ADKLGIPFYVWDFSDRFKADVIDDFVDSYAIGETPNPCLRCNEKIKFEALLDRSIALGFD 120 Query 121 TVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRG 180 V TGHYARL G +RR VD +KDQSYVL VLT +QL H FP+GDT K +IR EA G Sbjct 121 AVVTGHYARLHDGVMRRGVDANKDQSYVLGVLTDEQLAHCMFPVGDTIKPEIREEAKDHG 180 Query 181 LAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGL 240 VA+KPDSHDICFIP G T+AFLG++IG+R G++ D DG +A HDGV+GFTIGQR+GL Sbjct 181 FGVASKPDSHDICFIPDGQTQAFLGKKIGLRPGLMKDQDGSTVAEHDGVYGFTIGQRKGL 240 Query 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 G+ G +G+PRYVT IDA T TV +G DL V +T A +C VQV Sbjct 241 GLPREGLDGKPRYVTDIDAATGTVTIGQRDDLRVGGITADRLKRLDPAVHGREFECEVQV 300 Query 301 RAHGETVSAVAELIGDA------------------LFVQLHAPLRGVARGQTLVLYRPDP 342 RAHG V A A L+ D L + LH PL+GVARGQ V+Y+PD Sbjct 301 RAHGGVVPATARLVDDPERTTPAGRVKKEDESPWRLELDLHEPLQGVARGQAAVVYQPDA 360 Query 343 AGDEVLGSATIAGASGLSTGGNPG 366 GD +LGS TI + S G G Sbjct 361 DGDILLGSGTIRATAPWSDAGAAG 384 >gi|205822051|sp|A4FMT5.2|MNMA_SACEN RecName: Full=tRNA-specific 2-thiouridylase mnmA Length=362 Score = 430 bits (1106), Expect = 2e-118, Method: Compositional matrix adjust. Identities = 222/359 (62%), Positives = 269/359 (75%), Gaps = 5/359 (1%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 M+VLAAMSGGVDS+VAAAR V+AGHEVVGVH+ALS PGTLRTG+RGCC+ ED+ DARR Sbjct 1 MRVLAAMSGGVDSAVAAARAVEAGHEVVGVHLALSAKPGTLRTGARGCCTIEDSHDARRA 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 AD+LGIPFYVWDFAE+F E+VI FV YA G TPNPC+ CN++IKF AL +A+ALGFD Sbjct 61 ADILGIPFYVWDFAERFTEEVIETFVGEYAAGRTPNPCLTCNEKIKFEALLEKAMALGFD 120 Query 121 TVATGHYARLSGGR----LRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEA 176 V TGHYARL+ LRR+ D KDQSYVLA LTA+QL H+ FP+GD+ K Q+R EA Sbjct 121 AVCTGHYARLTVEDGVPVLRRSRDEGKDQSYVLASLTAEQLGHSMFPLGDSLKSQVRQEA 180 Query 177 ARRGLAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDAD-GVVLASHDGVHGFTIG 235 A RGLAVA KPDSHDICFIP G+TK FL ++G + G +VDA+ G VL H GVHGFT+G Sbjct 181 AERGLAVAKKPDSHDICFIPDGDTKKFLESKLGQKPGQLVDAETGAVLGEHTGVHGFTVG 240 Query 236 QRRGLGIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVD 295 QR+GLGI P P+GRPRYV +++ + TV VG L V + + P++ + GP + Sbjct 241 QRKGLGIDAPAPDGRPRYVLSLEPVSGTVKVGSADHLGVTEIDAKRPIWPSQRELDGPTE 300 Query 296 CVVQVRAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIA 354 CVVQVRAHG T AVAE+ + ++L PLRGVA GQ +VLYRP+ GD VLGSA IA Sbjct 301 CVVQVRAHGGTADAVAEVADGGMSIRLRRPLRGVAPGQAVVLYRPEEEGDLVLGSALIA 359 >gi|257054918|ref|YP_003132750.1| tRNA-specific 2-thiouridylase MnmA [Saccharomonospora viridis DSM 43017] gi|256584790|gb|ACU95923.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Saccharomonospora viridis DSM 43017] Length=363 Score = 430 bits (1105), Expect = 2e-118, Method: Compositional matrix adjust. Identities = 231/360 (65%), Positives = 271/360 (76%), Gaps = 6/360 (1%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 M+VLAAMSGGVDS+VAAAR VDAGH+VVGVH+ALS PGTLRTGSRGCCS ED+ DARR Sbjct 1 MRVLAAMSGGVDSAVAAARAVDAGHDVVGVHLALSAKPGTLRTGSRGCCSLEDSNDARRA 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 AD+LGIPFYVWDFAE+F E+V+ F+ YA G TPNPCV CN++IKF AL +AVALGFD Sbjct 61 ADILGIPFYVWDFAERFTEEVVETFIGEYAAGRTPNPCVTCNEKIKFEALLEKAVALGFD 120 Query 121 TVATGHYARLS----GGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEA 176 V TGHYARLS LRR+ D KDQSYVLA L +QLRHA FP+GD+ K ++RAEA Sbjct 121 AVCTGHYARLSVVDGVPELRRSADESKDQSYVLASLKPEQLRHAMFPLGDSRKPEVRAEA 180 Query 177 ARRGLAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDAD-GVVLASHDGVHGFTIG 235 A RGL VA KPDSHDICFIP G+TKAFL +R+G R G +VDA+ G VL H GVHGFTIG Sbjct 181 AERGLPVARKPDSHDICFIPDGDTKAFLEKRLGQRPGDLVDAETGAVLGRHTGVHGFTIG 240 Query 236 QRRGLGIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVD 295 QR+GLGI P P+G+PRYV A++ + TV VG L V + G P++ + +GP + Sbjct 241 QRKGLGIDAPAPDGKPRYVLALEPVSGTVKVGSGDKLAVNRIEGERPIWPSERPLAGPTE 300 Query 296 CVVQVRAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPD-PAGDEVLGSATIA 354 CV QVRAHG T AVAE++ D + V L PLRGVA GQ +VLYR D AGD VLGSA IA Sbjct 301 CVAQVRAHGGTAPAVAEVVDDRVVVHLREPLRGVAPGQVVVLYREDAEAGDVVLGSAKIA 360 >gi|256380038|ref|YP_003103698.1| tRNA-specific 2-thiouridylase MnmA [Actinosynnema mirum DSM 43827] gi|255924341|gb|ACU39852.1| tRNA(5-methylaminomethyl-2-thiouridylate)-methyl transferase [Actinosynnema mirum DSM 43827] Length=362 Score = 427 bits (1097), Expect = 2e-117, Method: Compositional matrix adjust. Identities = 226/359 (63%), Positives = 270/359 (76%), Gaps = 8/359 (2%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 M+VLAAMSGGVDS+VAAAR V+AGH+V GVH+ALS PGTLRTGSRGCC+ ED+ DARR Sbjct 1 MRVLAAMSGGVDSAVAAARAVEAGHDVTGVHLALSAKPGTLRTGSRGCCTIEDSHDARRA 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVLGIPFYVWDFAE+F EDV+ DFV+ YA G TPNPC+RCN++IKF AL +A+ALGFD Sbjct 61 ADVLGIPFYVWDFAERFTEDVVEDFVAEYAAGRTPNPCLRCNERIKFEALLDKAIALGFD 120 Query 121 TVATGHYARLS----GGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEA 176 V TGHYARL LRR+ D KDQSYVLA LT +QL HA FP+G + K ++RAEA Sbjct 121 AVCTGHYARLDVVDGSVELRRSADEGKDQSYVLASLTREQLAHAMFPLGGSTKVEVRAEA 180 Query 177 ARRGLAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDAD-GVVLASHDGVHGFTIG 235 ARRGL+VA KPDSHDICFIP G+T+ FL +R+G R GV+VD + G VL H GVHGFT+G Sbjct 181 ARRGLSVAAKPDSHDICFIPDGDTQGFLTKRLGQRAGVLVDDETGAVLGQHVGVHGFTVG 240 Query 236 QRRGLGIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVD 295 QR+GLGI P P+GRPRYV +++ + TV VG L V+ + PV SGPV+ Sbjct 241 QRKGLGIDAPAPDGRPRYVLSLEPVSGTVRVGAAEKLAVREIVAVRPVSRVDW--SGPVE 298 Query 296 CVVQVRAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDP-AGDEVLGSATI 353 CV QVRAHG T A+AEL+G+ L V+L PL GVA GQ + +YR DP GD VL SATI Sbjct 299 CVAQVRAHGGTAPAIAELVGEELVVRLREPLSGVAPGQAVAVYREDPEGGDLVLASATI 357 >gi|319949296|ref|ZP_08023374.1| tRNA-specific 2-thiouridylase MnmA [Dietzia cinnamea P4] gi|319437034|gb|EFV92076.1| tRNA-specific 2-thiouridylase MnmA [Dietzia cinnamea P4] Length=366 Score = 424 bits (1091), Expect = 9e-117, Method: Compositional matrix adjust. Identities = 232/360 (65%), Positives = 266/360 (74%), Gaps = 15/360 (4%) Query 11 VDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRVADVLGIPFYV 70 +DS+VAAAR V AGHEVVGVH+ALS+ P TLRTGSRGCCS+EDA DARRVAD+LGIPFYV Sbjct 1 MDSAVAAARAVAAGHEVVGVHLALSSDPATLRTGSRGCCSREDAGDARRVADLLGIPFYV 60 Query 71 WDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFDTVATGHYARL 130 WDFAE+F+ DVI+DFV SYARGETPNPC+RCN++IKF AL R +ALGFD VATGHYARL Sbjct 61 WDFAERFRADVIDDFVESYARGETPNPCLRCNERIKFEALLERGMALGFDAVATGHYARL 120 Query 131 S------GGR---LRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRGL 181 S G R LRRAVD KDQSYVL VLT +QL H+ FP+GD+ K Q+RAEA GL Sbjct 121 STEELPDGSRRPVLRRAVDAAKDQSYVLGVLTPEQLAHSMFPLGDSLKSQVRAEAEAAGL 180 Query 182 AVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDAD-GVVLASHDGVHGFTIGQRRGL 240 VA KPDSHDICFIPSG+T+AFLG RIGVR G VVDAD G L HDG+HGFTIGQR+GL Sbjct 181 PVATKPDSHDICFIPSGDTRAFLGARIGVRPGAVVDADTGDELGRHDGIHGFTIGQRKGL 240 Query 241 GIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV 300 G+ GP +GRPRYVTAIDAD+ TV VG L +T +P + DC VQ+ Sbjct 241 GVQGPAADGRPRYVTAIDADSGTVTVGGADHLLSVAMTATSPYWLVDDDIR-ETDCHVQI 299 Query 301 RAHGETVSAVAELIGD----ALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGA 356 RAHGE A IGD L + L P+RGVA GQ VLYR +P GD VLGS TI+ + Sbjct 300 RAHGEATPAHVRRIGDGADATLEITLDEPVRGVAPGQAAVLYRSEPDGDRVLGSGTISAS 359 >gi|296119548|ref|ZP_06838106.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Corynebacterium ammoniagenes DSM 20306] gi|295967431|gb|EFG80698.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Corynebacterium ammoniagenes DSM 20306] Length=361 Score = 424 bits (1089), Expect = 1e-116, Method: Compositional matrix adjust. Identities = 219/358 (62%), Positives = 258/358 (73%), Gaps = 6/358 (1%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 M+VL AMSGGVDSSVAAAR V+AGHEVVGVH+AL R +RGCCS ED+ADARR+ Sbjct 1 MRVLVAMSGGVDSSVAAARAVEAGHEVVGVHLALHKDAQQTREKARGCCSLEDSADARRI 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 D LGIPFYVWDF+E+FKE VI DFV SYARGETPNPC+RCN++IKFAAL + +ALGFD Sbjct 61 CDKLGIPFYVWDFSEEFKEAVITDFVDSYARGETPNPCLRCNEKIKFAALLRKGMALGFD 120 Query 121 TVATGHYARL-SGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARR 179 VATGHYA L G +RR++D +KDQSYVL V+TA++L + FPIGDTPK QIR EA R Sbjct 121 AVATGHYATLDKDGYMRRSLDENKDQSYVLGVITAEELSYCYFPIGDTPKPQIREEAKRH 180 Query 180 GLAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRG 239 G + A+KPDS+DICFIP GNT+AFLG RIG+R G++VD DG L HDG +TIGQR+G Sbjct 181 GFSTASKPDSYDICFIPDGNTQAFLGARIGMRPGMIVDQDGTELREHDGAWNYTIGQRKG 240 Query 240 LGIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQ 299 L I P +GRPRYVT IDA T TV VG L V ++ + A G DC VQ Sbjct 241 LDIKAPAADGRPRYVTGIDAQTGTVTVGSREALKVTRISADRLKYLHPAM-DGEFDCDVQ 299 Query 300 VRAHGETVSAVAEL--IGDALFVQLHAPLRGVARGQTLVLYRPDP--AGDEVLGSATI 353 VRAHG VS AE+ DA+ + L+ PL GVA GQ VLY P P GD V+GS TI Sbjct 300 VRAHGSVVSCHAEVDRDADAMILTLNEPLSGVAPGQAAVLYFPSPDELGDIVIGSGTI 357 >gi|260577537|ref|ZP_05845477.1| probable tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Corynebacterium jeikeium ATCC 43734] gi|258604341|gb|EEW17578.1| probable tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Corynebacterium jeikeium ATCC 43734] Length=389 Score = 423 bits (1087), Expect = 2e-116, Method: Compositional matrix adjust. Identities = 218/378 (58%), Positives = 264/378 (70%), Gaps = 18/378 (4%) Query 7 MSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRVADVLGI 66 MSGGVDS+VAA+R ++AGHEV+GVH+ALS +P +R GSRGCCS ED+ADARRVAD LGI Sbjct 1 MSGGVDSAVAASRALEAGHEVIGVHLALSQSPEAVRAGSRGCCSLEDSADARRVADKLGI 60 Query 67 PFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFDTVATGH 126 PFYVWDF+++FK DVI+DFV SYA GETPNPC+RCN++IKF AL R++ALGFD V TGH Sbjct 61 PFYVWDFSDRFKADVIDDFVDSYAIGETPNPCLRCNEKIKFEALLDRSIALGFDAVVTGH 120 Query 127 YARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRGLAVANK 186 YARL G +RR VD +KDQSYVL VLT +QL H FP+GDT K +IR EA G VA+K Sbjct 121 YARLHDGVMRRGVDANKDQSYVLGVLTDEQLAHCMFPVGDTIKPEIREEAKDHGFGVASK 180 Query 187 PDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGLGIAGPG 246 PDSHDICFIP G T+AFLG++IG+R G++ D DG +A HDGV+GFTIGQR+GLG+ G Sbjct 181 PDSHDICFIPDGQTQAFLGKKIGLRPGLMKDQDGSTVAEHDGVYGFTIGQRKGLGLPREG 240 Query 247 PNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQVRAHGET 306 +G+PRYVT IDA T TV +G DL V +T A +C VQVRAHG Sbjct 241 LDGKPRYVTDIDAATGTVTIGQRDDLRVGGITADRLKRLDPAVHGREFECEVQVRAHGGV 300 Query 307 VSAVAELIGDA------------------LFVQLHAPLRGVARGQTLVLYRPDPAGDEVL 348 V A A L+ D L + LH PL+GVARGQ V+Y+PD GD +L Sbjct 301 VPATARLVDDPERTTPAGRVKKEGESPWRLELDLHEPLQGVARGQAAVVYQPDAEGDILL 360 Query 349 GSATIAGASGLSTGGNPG 366 GS TI + S G G Sbjct 361 GSGTIRATAPWSDAGAAG 378 >gi|302865761|ref|YP_003834398.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Micromonospora aurantiaca ATCC 27029] gi|315502317|ref|YP_004081204.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Micromonospora sp. L5] gi|302568620|gb|ADL44822.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Micromonospora aurantiaca ATCC 27029] gi|315408936|gb|ADU07053.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Micromonospora sp. L5] Length=358 Score = 422 bits (1084), Expect = 5e-116, Method: Compositional matrix adjust. Identities = 223/358 (63%), Positives = 270/358 (76%), Gaps = 2/358 (0%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 M+VLAAMSGGVDS+VAAAR V+AGH+V GVH+AL+ P T RTG+RGCC+ ED+ DARR Sbjct 1 MRVLAAMSGGVDSAVAAARAVEAGHDVTGVHLALARNPQTYRTGARGCCTLEDSRDARRA 60 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 ADVLGIPFYVWD A++F +DV++DFV+ YA G TPNPC+RCN++IKFAA+ RAVALGFD Sbjct 61 ADVLGIPFYVWDMADRFHDDVVDDFVAEYAAGRTPNPCLRCNEKIKFAAVLDRAVALGFD 120 Query 121 TVATGHYARL-SGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARR 179 V TGH+ARL + G LRR+VD KDQSYVLAVLT QL + FP+GD+ K ++RAEAARR Sbjct 121 AVVTGHHARLGADGLLRRSVDAAKDQSYVLAVLTRAQLDRSIFPLGDSTKAEVRAEAARR 180 Query 180 GLAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDA-DGVVLASHDGVHGFTIGQRR 238 GLAVA+KPDSHDICFI G+T+ FL ER+G G VVDA G V+ SH G + +T+GQRR Sbjct 181 GLAVADKPDSHDICFIADGDTRGFLAERLGEAPGQVVDALTGAVVGSHSGAYQYTVGQRR 240 Query 239 GLGIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVV 298 GL + P P+GRPRYV +I T TV VG L+V + PV+T G P GPV+C V Sbjct 241 GLHLDRPAPDGRPRYVLSITPTTNTVTVGPAEALEVSDVRATRPVWTGGPRPDGPVECEV 300 Query 299 QVRAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGA 356 Q+RAHG+ V A E+ D L L P+RGVA GQ +V YRPDPAGD VLGSATI A Sbjct 301 QLRAHGDVVPAAVEVDADGLRASLRRPVRGVAAGQAIVAYRPDPAGDVVLGSATITAA 358 >gi|255324545|ref|ZP_05365662.1| tRNA-methyltransferase [Corynebacterium tuberculostearicum SK141] gi|255298451|gb|EET77751.1| tRNA-methyltransferase [Corynebacterium tuberculostearicum SK141] Length=365 Score = 421 bits (1083), Expect = 7e-116, Method: Compositional matrix adjust. Identities = 213/362 (59%), Positives = 258/362 (72%), Gaps = 6/362 (1%) Query 1 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRV 60 M+VL AMSGGVDSSVAAAR V+AGHEV+GVH+AL R +RGCCS ED+ADARR+ Sbjct 5 MRVLVAMSGGVDSSVAAARAVEAGHEVIGVHLALHKDAQQTREKARGCCSLEDSADARRI 64 Query 61 ADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFD 120 D LGIPFYVWDF+E+FKE+VI DFV SYARGETPNPC+RCN++IKF AL + +ALGFD Sbjct 65 CDKLGIPFYVWDFSEEFKEEVITDFVDSYARGETPNPCLRCNEKIKFRALLQKGMALGFD 124 Query 121 TVATGHYARL-SGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARR 179 VATGHYA + G +RR++D +KDQSYVL V++A++L H FPIGDTPK QIR EAA Sbjct 125 AVATGHYATIDEDGYMRRSLDENKDQSYVLGVISAEELEHCFFPIGDTPKPQIREEAAAH 184 Query 180 GLAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRG 239 G + A KPDS+DICFIP GNT+AFLG RIG+R G++VD +G L HDG +TIGQR+G Sbjct 185 GFSTAKKPDSYDICFIPDGNTQAFLGARIGLRPGMIVDQEGTELKEHDGAWNYTIGQRKG 244 Query 240 LGIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQ 299 L I P +GRPRYVT IDA T TV VG DL V ++ + G +C VQ Sbjct 245 LDIKAPAADGRPRYVTDIDAATGTVTVGAREDLAVTSIQADLLKYLHPGM-DGDFECEVQ 303 Query 300 VRAHGETVSAVAEL--IGDALFVQLHAPLRGVARGQTLVLYRPDP--AGDEVLGSATIAG 355 VRAHG V +A + D + ++L PL GVARGQ VLY P P GD V+GS TI G Sbjct 304 VRAHGSVVPCIAHVDREADEMRLELKEPLSGVARGQAAVLYLPSPDELGDIVIGSGTICG 363 Query 356 AS 357 + Sbjct 364 TN 365 >gi|291004692|ref|ZP_06562665.1| tRNA-specific 2-thiouridylase MnmA [Saccharopolyspora erythraea NRRL 2338] Length=356 Score = 421 bits (1083), Expect = 7e-116, Method: Compositional matrix adjust. Identities = 217/353 (62%), Positives = 263/353 (75%), Gaps = 5/353 (1%) Query 7 MSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRVADVLGI 66 MSGGVDS+VAAAR V+AGHEVVGVH+ALS PGTLRTG+RGCC+ ED+ DARR AD+LGI Sbjct 1 MSGGVDSAVAAARAVEAGHEVVGVHLALSAKPGTLRTGARGCCTIEDSHDARRAADILGI 60 Query 67 PFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFDTVATGH 126 PFYVWDFAE+F E+VI FV YA G TPNPC+ CN++IKF AL +A+ALGFD V TGH Sbjct 61 PFYVWDFAERFTEEVIETFVGEYAAGRTPNPCLTCNEKIKFEALLEKAMALGFDAVCTGH 120 Query 127 YARLSGGR----LRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRGLA 182 YARL+ LRR+ D KDQSYVLA LTA+QL H+ FP+GD+ K Q+R EAA RGLA Sbjct 121 YARLTVEDGVPVLRRSRDEGKDQSYVLASLTAEQLGHSMFPLGDSLKSQVRQEAAERGLA 180 Query 183 VANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDAD-GVVLASHDGVHGFTIGQRRGLG 241 VA KPDSHDICFIP G+TK FL ++G + G +VDA+ G VL H GVHGFT+GQR+GLG Sbjct 181 VAKKPDSHDICFIPDGDTKKFLESKLGQKPGQLVDAETGAVLGEHTGVHGFTVGQRKGLG 240 Query 242 IAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQVR 301 I P P+GRPRYV +++ + TV VG L V + + P++ + GP +CVVQVR Sbjct 241 IDAPAPDGRPRYVLSLEPVSGTVKVGSADHLGVTEIDAKRPIWPSQRELDGPTECVVQVR 300 Query 302 AHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIA 354 AHG T AVAE+ + ++L PLRGVA GQ +VLYRP+ GD VLGSA IA Sbjct 301 AHGGTADAVAEVADGGMSIRLRRPLRGVAPGQAVVLYRPEEEGDLVLGSALIA 353 Lambda K H 0.320 0.136 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Effective search space used: 694383994300 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects Posted date: Sep 5, 2011 4:36 AM Number of letters in database: 5,219,829,388 Number of sequences in database: 15,229,318 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 11 Window for multiple hits: 40