BLAST Search Results

BLASTP 2.2.25+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,229,318 sequences; 5,219,829,388 total letters Query= Rv3089 Length=503 Score E Sequences producing significant alignments: (Bits) Value gi|15610226|ref|NP_217605.1| chain-fatty-acid-CoA ligase [Mycoba... 1029 0.0 gi|254365716|ref|ZP_04981761.1| fatty-acid-CoA ligase fadD13 [My... 1027 0.0 gi|298526561|ref|ZP_07013970.1| substrate-CoA ligase [Mycobacter... 1026 0.0 gi|340628080|ref|YP_004746532.1| putative chain-fatty-acid-CoA l... 1023 0.0 gi|289571294|ref|ZP_06451521.1| fatty-acid-CoA ligase fadD13 [My... 977 0.0 gi|183982607|ref|YP_001850898.1| fatty-acid-CoA ligase FadD13 [M... 779 0.0 gi|289759214|ref|ZP_06518592.1| fatty-acid-CoA ligase fadD13 [My... 575 6e-162 gi|240171805|ref|ZP_04750464.1| AMP-dependent synthetase and lig... 443 3e-122 gi|218781879|ref|YP_002433197.1| AMP-dependent synthetase and li... 432 6e-119 gi|88706228|ref|ZP_01103935.1| long-chain fatty-acid-CoA ligase ... 429 7e-118 gi|254515530|ref|ZP_05127590.1| feruloyl-CoA synthetase [gamma p... 425 7e-117 gi|254482082|ref|ZP_05095324.1| AMP-binding enzyme, putative [ma... 404 3e-110 gi|218782659|ref|YP_002433977.1| AMP-dependent synthetase and li... 380 3e-103 gi|218780924|ref|YP_002432242.1| AMP-dependent synthetase and li... 369 6e-100 gi|302341943|ref|YP_003806472.1| AMP-dependent synthetase and li... 361 1e-97 gi|290956861|ref|YP_003488043.1| acid-thiol ligase [Streptomyces... 353 4e-95 gi|218781330|ref|YP_002432648.1| AMP-dependent synthetase and li... 353 5e-95 gi|226312168|ref|YP_002772062.1| acyl-CoA synthetase [Brevibacil... 350 3e-94 gi|326328680|ref|ZP_08195020.1| O-succinylbenzoate-CoA ligase [N... 345 1e-92 gi|345011968|ref|YP_004814322.1| O-succinylbenzoate-CoA ligase [... 343 5e-92 gi|297203702|ref|ZP_06921099.1| O-succinylbenzoate-CoA ligase [S... 341 2e-91 gi|9971912|gb|AAG10474.1|AF279106_36 predicted acid--CoA ligase ... 338 1e-90 gi|311029638|ref|ZP_07707728.1| long-chain-fatty-acid--CoA ligas... 338 1e-90 gi|88319806|emb|CAH10130.1| feruloyl-CoA synthetase [Streptomyce... 337 4e-90 gi|333370466|ref|ZP_08462468.1| long-chain-fatty-acid-CoA ligase... 335 1e-89 gi|68510506|gb|AAY98502.1| putative feruloyl-CoA synthetase [Rho... 332 7e-89 gi|316932178|ref|YP_004107160.1| AMP-dependent synthetase and li... 332 9e-89 gi|39933820|ref|NP_946096.1| putative acid-CoA ligase [Rhodopseu... 330 3e-88 gi|91978271|ref|YP_570930.1| AMP-dependent synthetase and ligase... 330 5e-88 gi|290956085|ref|YP_003487267.1| substrate-CoA ligase [Streptomy... 329 5e-88 gi|192289242|ref|YP_001989847.1| AMP-dependent synthetase and li... 328 1e-87 gi|89098400|ref|ZP_01171284.1| acyl-CoA synthase [Bacillus sp. N... 328 1e-87 gi|288553808|ref|YP_003425743.1| AMP-dependent synthetase and li... 328 2e-87 gi|159900315|ref|YP_001546562.1| AMP-dependent synthetase and li... 327 4e-87 gi|119963319|ref|YP_946756.1| O-succinylbenzoate-CoA ligase [Art... 327 4e-87 gi|138894249|ref|YP_001124702.1| long-chain-fatty-acid--CoA liga... 324 2e-86 gi|196248155|ref|ZP_03146857.1| AMP-dependent synthetase and lig... 323 5e-86 gi|90417333|ref|ZP_01225259.1| putative fatty-acid--coa ligase t... 323 5e-86 gi|90416827|ref|ZP_01224757.1| acyl-CoA synthetase [marine gamma... 323 6e-86 gi|301061414|ref|ZP_07202184.1| AMP-binding enzyme [delta proteo... 322 9e-86 gi|86751180|ref|YP_487676.1| AMP-dependent synthetase and ligase... 322 1e-85 gi|297531137|ref|YP_003672412.1| O-succinylbenzoate-CoA ligase [... 322 1e-85 gi|254391251|ref|ZP_05006456.1| feruloyl-CoA synthetase [Strepto... 322 1e-85 gi|114799539|ref|YP_762100.1| feruloyl-CoA synthetase [Hyphomona... 321 2e-85 gi|329937490|ref|ZP_08287048.1| O-succinylbenzoate-CoA ligase [S... 321 2e-85 gi|294816287|ref|ZP_06774930.1| Feruloyl-CoA synthetase [Strepto... 321 2e-85 gi|296269589|ref|YP_003652221.1| AMP-dependent synthetase and li... 320 2e-85 gi|56419203|ref|YP_146521.1| long-chain-fatty-acid--CoA ligase [... 320 3e-85 gi|302546432|ref|ZP_07298774.1| O-succinylbenzoate-CoA ligase [S... 320 3e-85 gi|291334294|gb|ADD93956.1| predicted acid CoA ligase fadD13 [un... 320 3e-85 >gi|15610226|ref|NP_217605.1| chain-fatty-acid-CoA ligase [Mycobacterium tuberculosis H37Rv] gi|15842659|ref|NP_337696.1| substrate--CoA ligase [Mycobacterium tuberculosis CDC1551] gi|31794268|ref|NP_856761.1| chain-fatty-acid-CoA ligase [Mycobacterium bovis AF2122/97] 70 more sequence titles Length=503 Score = 1029 bits (2660), Expect = 0.0, Method: Compositional matrix adjust. Identities = 503/503 (100%), Positives = 503/503 (100%), Gaps = 0/503 (0%) Query 1 MKNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALL 60 MKNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALL Sbjct 1 MKNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALL 60 Query 61 MPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRA 120 MPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRA Sbjct 61 MPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRA 120 Query 121 QADPPGTVTDWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHES 180 QADPPGTVTDWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHES Sbjct 121 QADPPGTVTDWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHES 180 Query 181 VHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQFDATKVWSLI 240 VHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQFDATKVWSLI Sbjct 181 VHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQFDATKVWSLI 240 Query 241 VEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVVQG 300 VEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVVQG Sbjct 241 VEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVVQG 300 Query 301 YALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGEGEVVIKSDILLK 360 YALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGEGEVVIKSDILLK Sbjct 301 YALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGEGEVVIKSDILLK 360 Query 361 EYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVIIG 420 EYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVIIG Sbjct 361 EYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVIIG 420 Query 421 VPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSEQQIVEYCGTRLARYKLPKKVIFAEAI 480 VPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSEQQIVEYCGTRLARYKLPKKVIFAEAI Sbjct 421 VPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSEQQIVEYCGTRLARYKLPKKVIFAEAI 480 Query 481 PRNPTGKILKTVLREQYSATVPK 503 PRNPTGKILKTVLREQYSATVPK Sbjct 481 PRNPTGKILKTVLREQYSATVPK 503 >gi|254365716|ref|ZP_04981761.1| fatty-acid-CoA ligase fadD13 [Mycobacterium tuberculosis str. Haarlem] gi|134151229|gb|EBA43274.1| fatty-acid-CoA ligase fadD13 [Mycobacterium tuberculosis str. Haarlem] Length=503 Score = 1027 bits (2655), Expect = 0.0, Method: Compositional matrix adjust. Identities = 502/503 (99%), Positives = 502/503 (99%), Gaps = 0/503 (0%) Query 1 MKNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALL 60 MKNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALL Sbjct 1 MKNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALL 60 Query 61 MPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRA 120 MPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRA Sbjct 61 MPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRA 120 Query 121 QADPPGTVTDWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHES 180 QADPPGTVTDWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHES Sbjct 121 QADPPGTVTDWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHES 180 Query 181 VHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQFDATKVWSLI 240 VHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQFDATKVWSLI Sbjct 181 VHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQFDATKVWSLI 240 Query 241 VEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVVQG 300 VEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVVQG Sbjct 241 VEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVVQG 300 Query 301 YALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGEGEVVIKSDILLK 360 YALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGEGEVVIKSDILLK Sbjct 301 YALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGEGEVVIKSDILLK 360 Query 361 EYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVIIG 420 EYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVIIG Sbjct 361 EYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVIIG 420 Query 421 VPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSEQQIVEYCGTRLARYKLPKKVIFAEAI 480 VPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSEQQIVEYCGTRLARYKLPKKV FAEAI Sbjct 421 VPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSEQQIVEYCGTRLARYKLPKKVTFAEAI 480 Query 481 PRNPTGKILKTVLREQYSATVPK 503 PRNPTGKILKTVLREQYSATVPK Sbjct 481 PRNPTGKILKTVLREQYSATVPK 503 >gi|298526561|ref|ZP_07013970.1| substrate-CoA ligase [Mycobacterium tuberculosis 94_M4241A] gi|298496355|gb|EFI31649.1| substrate-CoA ligase [Mycobacterium tuberculosis 94_M4241A] Length=503 Score = 1026 bits (2654), Expect = 0.0, Method: Compositional matrix adjust. Identities = 502/503 (99%), Positives = 502/503 (99%), Gaps = 0/503 (0%) Query 1 MKNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALL 60 MKNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALL Sbjct 1 MKNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALL 60 Query 61 MPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRA 120 MPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRA Sbjct 61 MPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRA 120 Query 121 QADPPGTVTDWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHES 180 QADPPGTVTDWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHES Sbjct 121 QADPPGTVTDWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHES 180 Query 181 VHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQFDATKVWSLI 240 VHSAASSWASTIDVRYRDRLLLPLPMFHVAAL TVIFSAMRGVTLISMPQFDATKVWSLI Sbjct 181 VHSAASSWASTIDVRYRDRLLLPLPMFHVAALATVIFSAMRGVTLISMPQFDATKVWSLI 240 Query 241 VEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVVQG 300 VEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVVQG Sbjct 241 VEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVVQG 300 Query 301 YALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGEGEVVIKSDILLK 360 YALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGEGEVVIKSDILLK Sbjct 301 YALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGEGEVVIKSDILLK 360 Query 361 EYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVIIG 420 EYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVIIG Sbjct 361 EYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVIIG 420 Query 421 VPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSEQQIVEYCGTRLARYKLPKKVIFAEAI 480 VPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSEQQIVEYCGTRLARYKLPKKVIFAEAI Sbjct 421 VPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSEQQIVEYCGTRLARYKLPKKVIFAEAI 480 Query 481 PRNPTGKILKTVLREQYSATVPK 503 PRNPTGKILKTVLREQYSATVPK Sbjct 481 PRNPTGKILKTVLREQYSATVPK 503 >gi|340628080|ref|YP_004746532.1| putative chain-fatty-acid-CoA ligase FADD13 [Mycobacterium canettii CIPT 140010059] gi|340006270|emb|CCC45446.1| putative chain-fatty-acid-CoA ligase FADD13 (fatty-acyl-CoA synthetase) [Mycobacterium canettii CIPT 140010059] Length=503 Score = 1023 bits (2644), Expect = 0.0, Method: Compositional matrix adjust. Identities = 499/503 (99%), Positives = 501/503 (99%), Gaps = 0/503 (0%) Query 1 MKNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALL 60 MKNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALL Sbjct 1 MKNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALL 60 Query 61 MPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRA 120 MPNSVEFCCLFYGAAKLGAVAVP+NTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIR Sbjct 61 MPNSVEFCCLFYGAAKLGAVAVPVNTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRT 120 Query 121 QADPPGTVTDWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHES 180 QADPPGTVTDWIGADSLAERLR AAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTH+S Sbjct 121 QADPPGTVTDWIGADSLAERLRPAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHDS 180 Query 181 VHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQFDATKVWSLI 240 VHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQFDATKVWSLI Sbjct 181 VHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQFDATKVWSLI 240 Query 241 VEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVVQG 300 VEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVVQG Sbjct 241 VEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVVQG 300 Query 301 YALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGEGEVVIKSDILLK 360 YALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGEGEVVIKSDILLK Sbjct 301 YALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGEGEVVIKSDILLK 360 Query 361 EYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVIIG 420 EYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVIIG Sbjct 361 EYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVIIG 420 Query 421 VPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSEQQIVEYCGTRLARYKLPKKVIFAEAI 480 VPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSEQQIVEYCGTRLARYKLPKKVIFAEAI Sbjct 421 VPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSEQQIVEYCGTRLARYKLPKKVIFAEAI 480 Query 481 PRNPTGKILKTVLREQYSATVPK 503 PRNPTGKILKTVLREQYSATVPK Sbjct 481 PRNPTGKILKTVLREQYSATVPK 503 >gi|289571294|ref|ZP_06451521.1| fatty-acid-CoA ligase fadD13 [Mycobacterium tuberculosis T17] gi|289545048|gb|EFD48696.1| fatty-acid-CoA ligase fadD13 [Mycobacterium tuberculosis T17] Length=503 Score = 977 bits (2525), Expect = 0.0, Method: Compositional matrix adjust. Identities = 482/503 (96%), Positives = 486/503 (97%), Gaps = 0/503 (0%) Query 1 MKNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALL 60 MKNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALL Sbjct 1 MKNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALL 60 Query 61 MPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRA 120 MPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRA Sbjct 61 MPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRA 120 Query 121 QADPPGTVTDWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHES 180 QADPPGTVTDWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHES Sbjct 121 QADPPGTVTDWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHES 180 Query 181 VHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQFDATKVWSLI 240 VHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQFDATKVWSLI Sbjct 181 VHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQFDATKVWSLI 240 Query 241 VEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVVQG 300 VEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVVQG Sbjct 241 VEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVVQG 300 Query 301 YALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGEGEVVIKSDILLK 360 YALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGEGEVVIKSDILLK Sbjct 301 YALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGEGEVVIKSDILLK 360 Query 361 EYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVIIG 420 EYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVIIG Sbjct 361 EYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVIIG 420 Query 421 VPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSEQQIVEYCGTRLARYKLPKKVIFAEAI 480 VPGVSEVAVIGLPDEKWGEIAA + + + + RLARYKLPKKVIFAEAI Sbjct 421 VPGVSEVAVIGLPDEKWGEIAAPSSLPTRTRSASSRSSSTAEPRLARYKLPKKVIFAEAI 480 Query 481 PRNPTGKILKTVLREQYSATVPK 503 PRNPTGKILKTVLREQYSATVPK Sbjct 481 PRNPTGKILKTVLREQYSATVPK 503 >gi|183982607|ref|YP_001850898.1| fatty-acid-CoA ligase FadD13 [Mycobacterium marinum M] gi|183175933|gb|ACC41043.1| fatty-acid-CoA ligase FadD13 [Mycobacterium marinum M] Length=502 Score = 779 bits (2012), Expect = 0.0, Method: Compositional matrix adjust. Identities = 374/501 (75%), Positives = 422/501 (85%), Gaps = 4/501 (0%) Query 1 MKNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALL 60 MKNIG ML+QRA +SPRLQAYVEPST+VRM Y ++N L NRCA VL ALG+ KGDRVALL Sbjct 1 MKNIGAMLKQRAHISPRLQAYVEPSTNVRMDYTELNMLTNRCASVLRALGVGKGDRVALL 60 Query 61 MPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRA 120 MPNSV FCCLFYGAAKLGAVAVP+NTRLAAPE+ FIL+DSGS V+IY PV+ AI+ Sbjct 61 MPNSVAFCCLFYGAAKLGAVAVPLNTRLAAPELGFILADSGSTVLIYHQSLEPVVTAIKT 120 Query 121 QADPPGTVTDWI----GADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVH 176 + V W+ G +LA+RL +A A EP + GG DNLFIMYTSGTTG+PKGVVH Sbjct 121 ATERSHNVISWVPLSDGEGALAQRLETADAGEPICDSGGSDNLFIMYTSGTTGNPKGVVH 180 Query 177 THESVHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQFDATKV 236 TH+SVH+AASSW+ T+DVRY+DRLLLPLPMFHVAALTTVI AMRGVTL+SMPQFD K Sbjct 181 THDSVHTAASSWSLTVDVRYQDRLLLPLPMFHVAALTTVIMCAMRGVTLVSMPQFDPAKA 240 Query 237 WSLIVEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIE 296 WSLIV+ERV + GAVPAILNFMR VPEF ELDAP+FR FITGGAPMPEALI++YAAKN++ Sbjct 241 WSLIVDERVSLAGAVPAILNFMRLVPEFTELDAPEFRCFITGGAPMPEALIRMYAAKNMQ 300 Query 297 VVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGEGEVVIKSD 356 VVQGYALTESCGGGTLLL EDAL K GSAGRATMF DV VR DDG+I E G+GEVVIKSD Sbjct 301 VVQGYALTESCGGGTLLLGEDALAKVGSAGRATMFADVRVRTDDGMIAERGQGEVVIKSD 360 Query 357 ILLKEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIES 416 LLKEYWNRP+ATRDAF WFRTGDIGEID +GYLYIKDR+KDMIISGGEN+YPAEIE+ Sbjct 361 FLLKEYWNRPDATRDAFHGDWFRTGDIGEIDSDGYLYIKDRIKDMIISGGENIYPAEIEN 420 Query 417 VIIGVPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSEQQIVEYCGTRLARYKLPKKVIF 476 VI G+PGV+EVAVIGLPD+KWGEIA AIVV+DQ VSE IVE+C RLARYKLPK++IF Sbjct 421 VITGIPGVAEVAVIGLPDQKWGEIACAIVVSDQGAVSEAGIVEFCAARLARYKLPKRLIF 480 Query 477 AEAIPRNPTGKILKTVLREQY 497 +PRNP GK+LK LRE Y Sbjct 481 TATLPRNPAGKVLKRELRELY 501 >gi|289759214|ref|ZP_06518592.1| fatty-acid-CoA ligase fadD13 [Mycobacterium tuberculosis T85] gi|289714778|gb|EFD78790.1| fatty-acid-CoA ligase fadD13 [Mycobacterium tuberculosis T85] Length=279 Score = 575 bits (1482), Expect = 6e-162, Method: Compositional matrix adjust. Identities = 279/279 (100%), Positives = 279/279 (100%), Gaps = 0/279 (0%) Query 1 MKNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALL 60 MKNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALL Sbjct 1 MKNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALL 60 Query 61 MPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRA 120 MPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRA Sbjct 61 MPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRA 120 Query 121 QADPPGTVTDWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHES 180 QADPPGTVTDWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHES Sbjct 121 QADPPGTVTDWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHES 180 Query 181 VHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQFDATKVWSLI 240 VHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQFDATKVWSLI Sbjct 181 VHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQFDATKVWSLI 240 Query 241 VEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGG 279 VEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGG Sbjct 241 VEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGG 279 >gi|240171805|ref|ZP_04750464.1| AMP-dependent synthetase and ligase [Mycobacterium kansasii ATCC 12478] Length=513 Score = 443 bits (1140), Expect = 3e-122, Method: Compositional matrix adjust. Identities = 236/514 (46%), Positives = 333/514 (65%), Gaps = 15/514 (2%) Query 3 NIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLMP 62 N+G LR+RA ++P L+A V+ + R T+A+++ A+R A VLT G+ KGDRVA+L+P Sbjct 2 NVGSHLRKRAELNPALEALVDDTAGRRFTFAELDERADRAARVLTGPGLGKGDRVAVLLP 61 Query 63 NSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAI--RA 120 +F +FYGAA+ G V VP+N RL A E++F+L DSG+ V+++ A V+ + R Sbjct 62 TCHQFVEVFYGAARAGLVVVPLNWRLVANELAFMLRDSGATVLVFDAEYDAVVAELHERG 121 Query 121 QADPPGTVTDW--IGADSLA-----ERLRSAAADEP-AVECGGDDNLFIMYTSGTTGHPK 172 D VT W +GAD A + L AA +P +V G D LFIMYTSGTTG PK Sbjct 122 GNDDGTPVTRWLRVGADGPAWAADFDALVDQAAPQPVSVVADGADPLFIMYTSGTTGLPK 181 Query 173 GVVHTHESVHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQFD 232 G VHTH+SV + +++D+R+RDR L+ LP+FHVAAL ++ RG T++ + QFD Sbjct 182 GAVHTHDSVQWCLLTMLASVDMRFRDRYLISLPLFHVAALAPLVGCVYRGATIVMLRQFD 241 Query 233 ATKVWSLIVEERVCIGGAVPAILNFMRQV--PEFAELDAPDFRYFITGGAPMPEALIKIY 290 ++W + +E++ AVPA+LNFM PE E A R+ ++G +P+P +LIK Y Sbjct 242 PKRIWEVFRDEQITTTLAVPAMLNFMLPTYQPELRESLA--LRWIMSGASPVPPSLIKSY 299 Query 291 AAKNIEVVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHG-EG 349 A EV Q Y LTESCG G ++ +DA+ GS G+A TD+ + + GV G G Sbjct 300 ADLGFEVHQVYGLTESCGPGCVIGPDDAMSHIGSTGKAFFHTDIRIVDEHGVDVASGVAG 359 Query 350 EVVIKSDILLKEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENV 409 E++I+ ++ YWNRPEAT + +GW TGDI D +G++YI+DR+KDMIISGGENV Sbjct 360 EILIRGRHIMAGYWNRPEATAETIVDGWLHTGDIAVRDADGFVYIQDRIKDMIISGGENV 419 Query 410 YPAEIESVIIGVPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSEQQIVEYCGTRLARYK 469 YPAEIE V++ PG+++VAV+G+P KWGE A+VV E+ E ++ +C +LARYK Sbjct 420 YPAEIEDVLLSHPGIADVAVVGIPSAKWGESPLAVVVRADPELDEAAVLVHCEGKLARYK 479 Query 470 LPKKVIFAEAIPRNPTGKILKTVLREQYSATVPK 503 LPK+ +F +AIPR PTGK LK VLREQ+S P+ Sbjct 480 LPKRAVFVDAIPRTPTGKALKRVLREQFSVAAPE 513 >gi|218781879|ref|YP_002433197.1| AMP-dependent synthetase and ligase [Desulfatibacillum alkenivorans AK-01] gi|218763263|gb|ACL05729.1| AMP-dependent CoA ligase/synthetase [Desulfatibacillum alkenivorans AK-01] Length=509 Score = 432 bits (1112), Expect = 6e-119, Method: Compositional matrix adjust. Identities = 220/509 (44%), Positives = 316/509 (63%), Gaps = 15/509 (2%) Query 2 KNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLM 61 N+G++L +RA + P +A+V+ +D+R++Y + NA ANR ADVL G+ KGDRV +LM Sbjct 3 NNVGYLLTKRADMLPDKEAFVDVQSDIRLSYKEYNANANRFADVLQKKGVKKGDRVGILM 62 Query 62 PNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRAQ 121 NS +F +F+ AK+GA+ VP+N RL E++FIL DSG+K++++G + IR Sbjct 63 MNSHQFMEMFFAIAKIGAICVPLNWRLVPDELTFILKDSGTKLLVFGDEFKDGVAVIRDN 122 Query 122 ADPPGT-VTDWI------GADSLAERLRSAAAD----EPAVECGGDDNLFIMYTSGTTGH 170 A GT V W+ A + A L + AD EP + DD +FIMYTSGTTG Sbjct 123 A---GTDVETWLHSGAAEDAPAFASNLETLKADASVAEPELGAFEDDPVFIMYTSGTTGL 179 Query 171 PKGVVHTHESVHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQ 230 PKG VHTH+++ A + ST ++R DR +PLP+FHV AL + + G+T+I++ Sbjct 180 PKGAVHTHKTLTWAVINMDSTWELRQSDRFFVPLPLFHVGALMPSVMAVYTGLTIIALKA 239 Query 231 FDATKVWSLIVEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIY 290 FD + W I E+V VP ++ M VPE D FR+ GAP+P +L++ Sbjct 240 FDPSLAWKTIEAEKVTNSLMVPTVMGAMLMVPEKDTCDVSSFRWSSIAGAPVPVSLLEAC 299 Query 291 AAKNIEVVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDV-AVRGDDGVIREHGEG 349 I + Q + LTE+ G G L+ + RK GSAG+ +F D V +D + + G Sbjct 300 KKIGINLEQLFGLTEAAGPGCFLIEDAVERKPGSAGKGCLFIDARVVDANDKPVPPNTPG 359 Query 350 EVVIKSDILLKEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENV 409 E+++K ++ YWNRPE T NGW TGD+ +DDEG++YI DR+KDMIISGGEN+ Sbjct 360 ELILKGKNMMVGYWNRPEDTEKTLKNGWLHTGDVATMDDEGFVYIVDRIKDMIISGGENI 419 Query 410 YPAEIESVIIGVPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSEQQIVEYCGTRLARYK 469 YPAEIE V+ G+PGV++VAVIG P EKWGE+ A++V E++E+ ++++C ++ARYK Sbjct 420 YPAEIEKVLAGMPGVAQVAVIGKPHEKWGEVPMAVIVPSDKELTEESVLQFCDGKIARYK 479 Query 470 LPKKVIFAEAIPRNPTGKILKTVLREQYS 498 LPK V F EA+P PTGK+ K VL Q S Sbjct 480 LPKAVTFVEALPMTPTGKVQKRVLERQLS 508 >gi|88706228|ref|ZP_01103935.1| long-chain fatty-acid-CoA ligase [Congregibacter litoralis KT71] gi|88699622|gb|EAQ96734.1| long-chain fatty-acid-CoA ligase [Congregibacter litoralis KT71] Length=526 Score = 429 bits (1102), Expect = 7e-118, Method: Compositional matrix adjust. Identities = 229/506 (46%), Positives = 313/506 (62%), Gaps = 9/506 (1%) Query 3 NIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLMP 62 NIG L R+ VSP+ + + + R ++ + N AN+ L+ +G+ GDRVALL Sbjct 17 NIGHFLTTRSRVSPQQEMLYDIKAERRFSFREANHRANQLCHALSGMGLEPGDRVALLAY 76 Query 63 NSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRAQA 122 N +F F+G AK G V +P+N RL A E++FIL D G+K +I+ + + +R + Sbjct 77 NGHQFIESFFGPAKAGLVIMPLNWRLTADELAFILKDGGAKALIFDTDFTHLAEDLRGRD 136 Query 123 DPPGTVTDWIGADSLA-------ERLRSAA-ADEPAVECGGDDNLFIMYTSGTTGHPKGV 174 V WI + A ERL + ADEP G +DNLFIMYTSGTTG PKGV Sbjct 137 VAGSDVEHWIAVGAEAPDFARHYERLLAQQPADEPEFLAGPEDNLFIMYTSGTTGLPKGV 196 Query 175 VHTHESVHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQFDAT 234 VHTHE+V A + +T D+R DR LL LP+FHV AL +I + RG +L+ + FD Sbjct 197 VHTHETVFWAIITMVNTGDIRGTDRYLLLLPLFHVGALAPMIGAVYRGNSLVILRDFDPL 256 Query 235 KVWSLIVEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKN 294 KVW L ER+ AVPA+LNFM QVP + + D R I G AP+P A I Y Sbjct 257 KVWQLFESERIDTSLAVPAMLNFMLQVPGYEQYDHSSVRNIICGAAPVPVATINAYIDLG 316 Query 295 IEVVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGE-GEVVI 353 IE+ Q Y LTES G G L++ ED+L GSAGRA T+ + G GE GE+++ Sbjct 317 IEIHQVYGLTESGGPGCLIVGEDSLTHVGSAGRAFFHTEAKIVDAHGETVPAGETGEILL 376 Query 354 KSDILLKEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAE 413 + ++KEYWNRP+AT + +GW TGDI +D EG++ I DR KDMIISGGENVYPAE Sbjct 377 RGRHMMKEYWNRPDATAETLQDGWLHTGDIATMDAEGFVTICDRKKDMIISGGENVYPAE 436 Query 414 IESVIIGVPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSEQQIVEYCGTRLARYKLPKK 473 IE+V++ GV++ AVIGLP EKWGE A++VA +++++++ +C +LAR+K P Sbjct 437 IENVLMQHDGVADAAVIGLPSEKWGESPLAVIVAADEALTDRELMTFCQGKLARFKQPTA 496 Query 474 VIFAEAIPRNPTGKILKTVLREQYSA 499 V F ++IPRNP+GKILK +LR+Q+ A Sbjct 497 VRFVDSIPRNPSGKILKRLLRDQFLA 522 >gi|254515530|ref|ZP_05127590.1| feruloyl-CoA synthetase [gamma proteobacterium NOR5-3] gi|219675252|gb|EED31618.1| feruloyl-CoA synthetase [gamma proteobacterium NOR5-3] Length=515 Score = 425 bits (1093), Expect = 7e-117, Method: Compositional matrix adjust. Identities = 229/504 (46%), Positives = 308/504 (62%), Gaps = 9/504 (1%) Query 3 NIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLMP 62 NIG L RA ++PR + + R T+A+ NA N+ L+ +G+ GDRVA+L Sbjct 6 NIGQFLATRARMNPRQEMLYDVKAQRRFTFAESNARGNQLCRALSGMGLEPGDRVAVLAY 65 Query 63 NSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRAQA 122 N +F F+G AK+G V +P+N RL A E++FIL D G+K +I+ + + +RA+ Sbjct 66 NGHQFVESFFGPAKVGLVIMPLNWRLTADELAFILKDGGAKALIFDTDFTALAEDLRARG 125 Query 123 DPPGTVTDWIGADSLA-------ERLRSAA-ADEPAVECGGDDNLFIMYTSGTTGHPKGV 174 V WI S A ERL + DEPA +DNLFIMYTSGTTG PKGV Sbjct 126 ALGSDVEHWIAIGSEAPDFARHYERLLAQQPVDEPAGLAAAEDNLFIMYTSGTTGLPKGV 185 Query 175 VHTHESVHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQFDAT 234 VHTHE+V A + +T D+R DR LL LP+FHV AL +I + RG +++ + FD Sbjct 186 VHTHETVFWAIITMVNTGDIRGTDRYLLLLPLFHVGALAPMIGAVYRGNSMVILRDFDPQ 245 Query 235 KVWSLIVEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKN 294 KVW L ER+ AVPA+LNFM QVP + D R I G AP+P A I Y Sbjct 246 KVWELFESERIDTSLAVPAMLNFMLQVPGREKYDHSSVRNIICGAAPVPVATINAYIDLG 305 Query 295 IEVVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGE-GEVVI 353 IE+ Q Y LTES G G L++ ED+L GSAGRA T+ + G GE GE+++ Sbjct 306 IEIHQVYGLTESGGPGCLIVGEDSLAHVGSAGRAFFHTEAKIVDGRGNTVPTGEAGEILL 365 Query 354 KSDILLKEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAE 413 + ++KEYWNRP+AT + +GW TGDI +DDEG++ I DR KDMIISGGENVYPAE Sbjct 366 RGRHIMKEYWNRPDATAETLQDGWLHTGDIARMDDEGFITICDRKKDMIISGGENVYPAE 425 Query 414 IESVIIGVPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSEQQIVEYCGTRLARYKLPKK 473 IE+V++ GV++ AVIGLP EKWGE AI++ + + I+ +C +LAR+K P Sbjct 426 IENVLMQHEGVADAAVIGLPSEKWGESPLAIIIPKDPSLDDAAILSFCQGKLARFKQPTA 485 Query 474 VIFAEAIPRNPTGKILKTVLREQY 497 V F ++IPRNP+GKILK +LR+ + Sbjct 486 VRFVDSIPRNPSGKILKRLLRDTF 509 >gi|254482082|ref|ZP_05095324.1| AMP-binding enzyme, putative [marine gamma proteobacterium HTCC2148] gi|214037772|gb|EEB78437.1| AMP-binding enzyme, putative [marine gamma proteobacterium HTCC2148] Length=531 Score = 404 bits (1037), Expect = 3e-110, Method: Compositional matrix adjust. Identities = 216/523 (42%), Positives = 301/523 (58%), Gaps = 27/523 (5%) Query 1 MKNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALL 60 + N+G +L +RA ++P +A+VE + R T++++N +N+ A VL GI GDRVA L Sbjct 14 LTNVGHLLTKRAAITPHREAFVEWERNRRFTFSELNTRSNQIASVLLTQGIKPGDRVATL 73 Query 61 MPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAI-- 118 + N +EF ++ AK+GAV VP+N RL + E+S+IL DS + +++ A +D + Sbjct 74 LKNGIEFVESYFAIAKIGAVMVPVNWRLVSVEISYILKDSAASTLLFDAEFDNTVDELQS 133 Query 119 ---------------RAQADPPGTVTDWIGADSLAERLRSAAADEPAVECGGDDNLFIMY 163 R +A+ P DW A E EP GDDNLFIMY Sbjct 134 GKRGELPCQHWLRCSRGEAETP----DW--AQDYDELTAKGDISEPTTGASGDDNLFIMY 187 Query 164 TSGTTGHPKGVVHTHESVHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMRGV 223 TSGTTGHPKG VH+H+ + + + ST D+R DR LL LPMFHV L RG Sbjct 188 TSGTTGHPKGAVHSHDGMLWSQLTSMSTSDMRGDDRFLLALPMFHVGCLNPTSLLVHRGA 247 Query 224 TLISMPQFDATKVWSLIVEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMP 283 T + M D +++ I E++ I AVPA+L FM PE + D R+ TG AP+P Sbjct 248 TGVIMRDLDMPGMFNCIDAEKISIFMAVPALLQFMLHAPEREQCDISSVRWIATGAAPVP 307 Query 284 EALIKIYAAKNIEVVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAV---RGDD 340 +L+ +A + + Q Y LTESCG GTLLL EDA K GS GR M T++ + RG Sbjct 308 VSLLDAWAKLGVSIHQAYGLTESCGPGTLLLPEDAASKVGSCGRPQMHTELKIIDARGKT 367 Query 341 GVIREHGEGEVVIKSDILLKEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKD 400 + GE+++ ++KEYWN P+AT +A +GW TGDI D EG++ I DR KD Sbjct 368 IPMGSDEPGELLLAGRHMMKEYWNNPKATAEALIDGWLHTGDICTWDVEGFITICDRKKD 427 Query 401 MIISGGENVYPAEIESVIIGVPGVSEVAVIGLPDEKWGEIAAAIVVADQN-EVSEQQIVE 459 M+ISGGEN+YPAE+E+V+ P V E AVIG+P KWGE A+VVA + + + + Sbjct 428 MVISGGENIYPAELENVLAACPDVQEAAVIGIPSVKWGETPLALVVAATGASPTNETLQQ 487 Query 460 YCGTRLARYKLPKKVIFAEAIPRNPTGKILKTVLREQYSATVP 502 +C LA YK+P+ + +PRNP+GK+LK LREQY P Sbjct 488 WCKDHLAGYKVPQLYKIVDHLPRNPSGKLLKPKLREQYPGPAP 530 >gi|218782659|ref|YP_002433977.1| AMP-dependent synthetase and ligase [Desulfatibacillum alkenivorans AK-01] gi|218764043|gb|ACL06509.1| AMP-dependent CoA ligase/synthetase [Desulfatibacillum alkenivorans AK-01] Length=503 Score = 380 bits (976), Expect = 3e-103, Method: Compositional matrix adjust. Identities = 209/505 (42%), Positives = 304/505 (61%), Gaps = 15/505 (2%) Query 3 NIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLMP 62 NIG L +RA SP ++A V +R TY ++N +NR A+ + + GI GDRVA L Sbjct 4 NIGSFLTKRAQHSPNMEALV--IGGLRFTYKELNERSNRLANAMKSAGIEPGDRVAYLGL 61 Query 63 NSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRAQA 122 N EF L++G KLGA+ VP+N RLA PEV +I+++ + V+ G PVIDAI+ Sbjct 62 NETEFFDLYFGLGKLGAILVPVNFRLAPPEVLYIINNCEASKVVVGTDFFPVIDAIKGDL 121 Query 123 DPPG------TVTDWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVH 176 ++ +W A S ++ L S + DEP G DD L I+YTSGTTG PKG Sbjct 122 CTNSIYALGDSIPEW--AQSYSDFLASGSPDEPVHVGGDDDTLTILYTSGTTGKPKGAEL 179 Query 177 THES-VHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQFDATK 235 TH H A + A+ DV ++L+PLP+FH+ AL V G+T++ FD + Sbjct 180 THAGYFHEAVNLRATLGDVG--TKMLMPLPLFHIGALAPVPHCVQFGMTMVFQRAFDPAE 237 Query 236 VWSLIVEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNI 295 L+ E + G+VP +L F+R VP+F D R + AP+P LIK +A K + Sbjct 238 FLQLLATENISWFGSVPQVLMFLRSVPQFETFDWSSIRMALVYAAPVPVTLIKEFAEKGM 297 Query 296 EVVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGV-IREHGEGEVVIK 354 V Q Y +TE G ++ ++ A+ KAGS G A D+ + D G + GE+++ Sbjct 298 NVRQLYGMTECTGPAAVIDADKAIVKAGSTGPAMFHCDIKLVDDKGEEVPTGELGELLLL 357 Query 355 SDILLKEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEI 414 + +K YWN PEAT +GW +GD+ ++D++GYLYI DR KDMIISGGEN+YPAE+ Sbjct 358 TTHPMKGYWNNPEATASTIIDGWIHSGDMAKMDEDGYLYILDRKKDMIISGGENIYPAEV 417 Query 415 ESVIIGVPGVSEVAVIGLPDEKWGE-IAAAIVVADQNEVSEQQIVEYCGTRLARYKLPKK 473 E ++ P +++V VIG+ DEKWGE + A IV+ + +++ +++E+C +LAR+K PK+ Sbjct 418 EDTLLSHPAIADVGVIGVQDEKWGEAVKAVIVLNKEQSLTQDELIEWCRDKLARFKTPKQ 477 Query 474 VIFAEAIPRNPTGKILKTVLREQYS 498 V+FAE IPR PTGKILK +LR+QY+ Sbjct 478 VVFAEEIPRTPTGKILKRILRDQYN 502 >gi|218780924|ref|YP_002432242.1| AMP-dependent synthetase and ligase [Desulfatibacillum alkenivorans AK-01] gi|218762308|gb|ACL04774.1| Long-chain-fatty-acid CoA ligase (AMP-forming) [Desulfatibacillum alkenivorans AK-01] Length=505 Score = 369 bits (948), Expect = 6e-100, Method: Compositional matrix adjust. Identities = 206/501 (42%), Positives = 289/501 (58%), Gaps = 9/501 (1%) Query 3 NIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLMP 62 N+G +L RA +SP L+A V D R TYAQ NA ANR A L A KGDR+A+L Sbjct 4 NLGHILANRAFLSPNLEACV--GADYRYTYAQENARANRFASALKANDFKKGDRIAVLCK 61 Query 63 NSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRAQA 122 N+ C GAAK+G VA +N RL APE+ +IL++ G+ ++IY APV+D ++ Sbjct 62 NNEHIICALMGAAKMGVVAAILNWRLTAPELEYILNNCGADMLIYDDAFAPVVDELKGNI 121 Query 123 DPPGTVTDWI-GADS-LAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHES 180 V+ + G D + L + +EP++E GGDD IMYTSGTTG PKG + +H + Sbjct 122 PARLFVSKFAKGKDQDFEDFLAQGSEEEPSIEAGGDDPCVIMYTSGTTGKPKGAMISHNN 181 Query 181 VHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQFDATKVWSLI 240 A+ T+ Y++R LL P+FH+ L+ V + +G+T + MP FD ++ I Sbjct 182 AFWASLGLTHTLPWGYKERYLLVAPLFHIGGLSPVFANIHKGLTTVFMPDFDPVGMYKTI 241 Query 241 VEERVCIGGAVPAILNFMRQVPE--FAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVV 298 +ER+ VP +L M VP + D +F+ G +P+P +LI +Y K ++ Sbjct 242 QDERINFMMTVPLMLMAMAMVPPDVVEKFDLSSLNFFVCGASPVPPSLIHLYNEKGFKIA 301 Query 299 QGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAV--RGDDGVIREHGEGEVVIKSD 356 Q Y TE G T E + K SAG+ V V G D + GE+ + Sbjct 302 QVYGATEYTGAITFWTHEIGMDKCASAGKPVFHGQVKVCKPGSDEEVPAGEVGELCLFGP 361 Query 357 ILLKEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIES 416 + YWN P+A+ +A G +R+GD+G ID++G +Y+ DRLKDMIISGGEN+YPAEIE Sbjct 362 QVFLGYWNNPKASEEALTGGCYRSGDLGRIDEDGCVYVIDRLKDMIISGGENIYPAEIEQ 421 Query 417 VIIGVPGVSEVAVIGLPDEKWGEIAAAIVVADQNE-VSEQQIVEYCGTRLARYKLPKKVI 475 V+ P V+E AV G PDEKWGEI A V E ++E+ I++ C LA +K K+V Sbjct 422 VLQSHPAVAEAAVTGRPDEKWGEIPVAHVAVRPGETITEEDILQVCRDNLAGFKRVKEVH 481 Query 476 FAEAIPRNPTGKILKTVLREQ 496 F EA+P+N TGKILKTVLRE+ Sbjct 482 FVEALPKNSTGKILKTVLREE 502 >gi|302341943|ref|YP_003806472.1| AMP-dependent synthetase and ligase [Desulfarculus baarsii DSM 2075] gi|301638556|gb|ADK83878.1| AMP-dependent synthetase and ligase [Desulfarculus baarsii DSM 2075] Length=503 Score = 361 bits (927), Expect = 1e-97, Method: Compositional matrix adjust. Identities = 199/506 (40%), Positives = 294/506 (59%), Gaps = 15/506 (2%) Query 3 NIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLMP 62 NIG ++ +RA +SP + ++ R ++AQ+N+ N+ A L GI GDRVA+L Sbjct 4 NIGALISERARLSPDREGFI--GEGYRYSFAQVNSRVNQLAAYLAGRGIGFGDRVAVLCK 61 Query 63 NSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRAQA 122 N+ + AAKLGA+AV +N RL PE+ +IL D G+ ++Y PV+D +RA+ Sbjct 62 NNEHIGVALFAAAKLGAIAVMLNWRLQGPELEYILGDCGATTLLYDVDFLPVVDGLRAKI 121 Query 123 DPPGTVTDWI-----GADSLAERLRSAAAD-EPAVECGGDDNLFIMYTSGTTGHPKGVVH 176 V ++I G D+ E+ ++ D EP + GGDD IMYTSGTTG PKG V Sbjct 122 ----PVKNYIRRGGQGPDADYEQALASQPDAEPTLRGGGDDGAVIMYTSGTTGRPKGAVL 177 Query 177 THESVHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQFDATKV 236 TH ++ + + T+ Y R L+ P+FH+ L V+ + G T MP+FD KV Sbjct 178 THHNLLWQTMAISHTVRWYYDHRFLVVAPLFHIGGLAPVVTNVHEGSTSYFMPEFDPVKV 237 Query 237 WSLIVEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIE 296 W +I EE++ +VP ++ + V + +D+ G A +P++LI+ + A + Sbjct 238 WKVIAEEKITTMMSVPLMVQALYMVAQKMPVDSSSLLNITCGAAAVPKSLIQAFLAMGVR 297 Query 297 VVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGD-DGVIREHGE-GEVVIK 354 V Q Y TE G T E + S G+A T + + G G+ GE+ +K Sbjct 298 VQQVYGATEVSGSATFWTHEMDPERCDSQGKADFHTQIRIADPASGQTLPTGQVGEIWVK 357 Query 355 SDILLKEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEI 414 I+ KEYW +P+AT +A +GW+RTGD+G +DD+GY+++ DRLKDMIISGGEN+YPAE+ Sbjct 358 GPIVFKEYWGKPQATAEAKQDGWYRTGDMGRMDDKGYVFVVDRLKDMIISGGENIYPAEL 417 Query 415 ESVIIGVPGVSEVAVIGLPDEKWGEIAAA-IVVADQNEVSEQQIVEYCGTRLARYKLPKK 473 E+VI P V+EVAV+G DEKWGEI A + + + ++ ++I + C LA +K K+ Sbjct 418 EAVIASHPAVAEVAVVGRHDEKWGEIPVAYLALKPEAKLEAKEIFDLCRKNLAAFKCVKE 477 Query 474 VIFAEAIPRNPTGKILKTVLREQYSA 499 V F EA+PRNP GKILKT LR+ A Sbjct 478 VRFVEALPRNPVGKILKTTLRQMVDA 503 >gi|290956861|ref|YP_003488043.1| acid-thiol ligase [Streptomyces scabiei 87.22] gi|260646387|emb|CBG69482.1| putative acid-thiol ligase [Streptomyces scabiei 87.22] Length=494 Score = 353 bits (906), Expect = 4e-95, Method: Compositional matrix adjust. Identities = 205/475 (44%), Positives = 278/475 (59%), Gaps = 9/475 (1%) Query 30 MTYAQMNALANRCADVLTALGIAKGDRVALLMPNSVEFCCLFYGAAKLGAVAVPINTRLA 89 ++Y ++ A R A L AL + GDRVA L N + A LGAV VP+NTRLA Sbjct 15 LSYGELAARVTRLAHALRALEVGHGDRVAYLGANHPALAETLFAANTLGAVFVPLNTRLA 74 Query 90 APEVSFILSDSGSKVVIYGAPSAPVIDAIRAQADPPGTVTDWIGADSLAERLRSAAADEP 149 APE++FIL D+G+ ++Y A V++A+R + V ++ S E L + A D+P Sbjct 75 APELAFILRDAGAAALLYDAELTDVVEALRDDVE----VKHYVEVGSAYEELLANAPDDP 130 Query 150 AVECGG-DDNLFIMYTSGTTGHPKGVVHTHESVHSAASSWASTIDVRYRDRLLLPLPMFH 208 E G D+ I YTSGT+G PKGV+ TH ++ + +D+ D L+ PMFH Sbjct 131 LDETVGLDEVCMIQYTSGTSGRPKGVMLTHANIAWNCFNMLLDVDIVSDDVALVVAPMFH 190 Query 209 VAALTTVIFSA-MRGVTLISMPQFDATKVWSLIVEERVCIGGAVPAILNFMRQVPEFAEL 267 AAL TV ++G T + M FD +V +I RV VPA+ M + P +A Sbjct 191 TAALNTVFLPGFLKGGTSVLMRGFDPERVLDVIAGHRVTRMFGVPAMYQAMARSPRWATA 250 Query 268 DAPDFRYFITGGAPMPEALIKIYAAKNIEVVQGYALTESCGGGTLLLSEDALRKAGSAGR 327 D R AP+P+ALI Y + + +Q Y LTE+ L ++D++RKAGSAG Sbjct 251 DLSSVRILQCAAAPVPKALIHTYQDRGLTFLQAYGLTETAPAALGLRAQDSIRKAGSAGT 310 Query 328 ATMFTDVAVRGDDGVIREHGE-GEVVIKSDILLKEYWNRPEATRDAFDNG-WFRTGDIGE 385 FTDV V D GE GEV+I+ ++K YW PEAT+DAF G WFR+GD Sbjct 311 PCFFTDVRVVRPDLTDVAPGEVGEVIIEGLNVMKGYWQWPEATKDAFFQGSWFRSGDAAT 370 Query 386 IDDEGYLYIKDRLKDMIISGGENVYPAEIESVIIGVPGVSEVAVIGLPDEKWGEIAAAIV 445 +D+EGY+ I DR+KDM ISGGENVYPAE+E ++ P V+E AVIG+PDE+WGE+ A+V Sbjct 371 VDEEGYVTIVDRIKDMYISGGENVYPAEVEQLLHQHPAVTECAVIGVPDERWGEVGRALV 430 Query 446 VADQN-EVSEQQIVEYCGTRLARYKLPKKVIFAEAIPRNPTGKILKTVLREQYSA 499 V EVS I++ RLA+YKLPK V+ +A+PRN TGKILK LR QY + Sbjct 431 VPRAGAEVSPGDIMDSLSGRLAKYKLPKSVVLVDALPRNATGKILKARLRRQYGS 485 >gi|218781330|ref|YP_002432648.1| AMP-dependent synthetase and ligase [Desulfatibacillum alkenivorans AK-01] gi|218762714|gb|ACL05180.1| AMP-dependent CoA ligase/synthetase [Desulfatibacillum alkenivorans AK-01] Length=508 Score = 353 bits (905), Expect = 5e-95, Method: Compositional matrix adjust. Identities = 200/484 (42%), Positives = 278/484 (58%), Gaps = 16/484 (3%) Query 27 DVRMTYAQMNALANRCADVLTALGIAKGDRVALLMPNSVEFCCLFYGAAKLGAVAVPINT 86 D+R+ + N N+ A +L GI KGDRVA LM NS F + + +K+GA+ VP+N Sbjct 24 DLRLNKREFNERINQLAHLLQKEGIRKGDRVAALMANSNVFLEILFACSKIGAIMVPLNF 83 Query 87 RLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRAQADPPGTVTDWI-----GA--DSLAE 139 RLA PE+ FI++D ++ Y V DA++ D TV + I GA D E Sbjct 84 RLAPPELEFIINDCEPSLLFYSPEFTAVRDALQ---DKIPTVRERICEMAGGAPNDGEYE 140 Query 140 RL---RSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHESVHSAASSWASTIDVRY 196 L S A P E DD FIMYTSGTTG PKG V +H + A + A T Sbjct 141 SLIAPMSTAEPTPESEVVMDDPQFIMYTSGTTGKPKGAVLSHGNTQWNAINAAVTYGSDN 200 Query 197 RDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQF-DATKVWSLIVEERVCIGGAVPAIL 255 D ++ P+FH+ AL A+ G +++ +F D V +I E+RV + +P + Sbjct 201 NDVVMCCAPLFHIGALNCSATPALYGGASLTIQRFFDPVGVLKMIQEDRVTVMFGIPVMY 260 Query 256 NFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVVQGYALTESCGGGTLLLS 315 FM Q+PEF D +Y + GG+P P ALI+ Y K + QGY +TE+ + L Sbjct 261 LFMSQMPEFPNTDFSTVKYLLAGGSPCPRALIETYQKKGVLFAQGYGMTETAPAISALRK 320 Query 316 EDALRKAGSAGRATMFTDV-AVRGDDGVIREHGEGEVVIKSDILLKEYWNRPEATRDAFD 374 E+AL+K GS G+ + T+V V + + H GEVV++ I++ EYW RPEAT + Sbjct 321 EEALKKIGSCGKPLLHTEVKVVDAQNNELAPHEMGEVVVRGPIVMLEYWKRPEATANTIV 380 Query 375 NGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVIIGVPGVSEVAVIGLPD 434 +GW TGD+G D+EGYLY+ DR KDM ISGGENVYPAE+E ++ P +++ VIG+ D Sbjct 381 DGWMHTGDMGYFDEEGYLYLMDRKKDMYISGGENVYPAEVEDALMSNPKIADAGVIGVAD 440 Query 435 EKWGEIAAAIVVADQNE-VSEQQIVEYCGTRLARYKLPKKVIFAEAIPRNPTGKILKTVL 493 EKWGE+ AI+V E +SE +++ +C +LA YK PKKV F + +PR TGKILK L Sbjct 441 EKWGEVGMAILVKTPGEDLSEDEVISWCREKLAGYKCPKKVAFVDELPRTMTGKILKKDL 500 Query 494 REQY 497 R QY Sbjct 501 RAQY 504 >gi|226312168|ref|YP_002772062.1| acyl-CoA synthetase [Brevibacillus brevis NBRC 100599] gi|226095116|dbj|BAH43558.1| probable O-succinylbenzoate--CoA ligase [Brevibacillus brevis NBRC 100599] Length=508 Score = 350 bits (898), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 203/504 (41%), Positives = 284/504 (57%), Gaps = 17/504 (3%) Query 1 MKNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVL-TALGIAKGDRVAL 59 M I + + +RA ++P A + + + +TY QMN R A+ L L + KGDR+ + Sbjct 1 MHGIAYWIEKRARITPTRIAVI--ANEQSLTYQQMNEQIQRVANYLRHELQVKKGDRIGI 58 Query 60 LMPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIR 119 L NS+E+ L + AKLGA+AVP N RL E+ + DSG +V+ A I+ I+ Sbjct 59 LSQNSLEYVLLLFAIAKLGAIAVPFNIRLTPAELDYQFEDSGIRVLCAQPEFAATIELIK 118 Query 120 AQAD-------PPGTVTDWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPK 172 A+A PG + W D +E + + D A + GDD I YTSGTTG PK Sbjct 119 AKASVAHMIWLEPGELP-W-SQDGPSESSQHSRID--ADDGSGDDPYIICYTSGTTGKPK 174 Query 173 GVVHTHESVHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIF-SAMRGVTLISMPQF 231 G V T E++ + + +D+ D ++ LP+FH+ + F + + G ++ +F Sbjct 175 GAVLTQENMFWNSVHNVAALDLTSEDCSIVLLPLFHIGGIGLFAFPTWLAGGRVVVAGKF 234 Query 232 DATKVWSLIVEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYA 291 D K LI RV I VPAI +RQ P FA R+F GGAP P LI+ + Sbjct 235 DPDKAIQLIETHRVTIVMGVPAIHEAIRQSPLFATTSFDSVRWFYNGGAPCPMELIQHFQ 294 Query 292 AKNIEVVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGE-GE 350 + + QGY LTE+ ++ EDA RKAGS G+ MF +V + DDG GE GE Sbjct 295 ERGLPFGQGYGLTETSPTVFMIAKEDARRKAGSIGKPVMFCEVRLISDDGKDVGQGEIGE 354 Query 351 VVIKSDILLKEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVY 410 +++K ++KEYWNRPE T + +GW TGD+ D+EG+ YI R KDMIISGGEN+Y Sbjct 355 LLVKGPNVMKEYWNRPEETANTIRDGWLYTGDLARFDEEGFAYIVGRRKDMIISGGENIY 414 Query 411 PAEIESVIIGVPGVSEVAVIGLPDEKWGEIAAAIVVADQNEV-SEQQIVEYCGTRLARYK 469 P E+E VI + GV E AV+G+PDEKWGE+A A +V V +E I E+C RLA+YK Sbjct 415 PLELEQVIGELDGVQEAAVVGVPDEKWGEVAKAFIVPKSGAVITEAHIKEHCEQRLAKYK 474 Query 470 LPKKVIFAEAIPRNPTGKILKTVL 493 +PK +F +PRN TGKI+K L Sbjct 475 IPKSFVFLTELPRNATGKIVKQAL 498 >gi|326328680|ref|ZP_08195020.1| O-succinylbenzoate-CoA ligase [Nocardioidaceae bacterium Broad-1] gi|326328683|ref|ZP_08195022.1| O-succinylbenzoate-CoA ligase [Nocardioidaceae bacterium Broad-1] gi|325953493|gb|EGD45494.1| O-succinylbenzoate-CoA ligase [Nocardioidaceae bacterium Broad-1] gi|325953641|gb|EGD45641.1| O-succinylbenzoate-CoA ligase [Nocardioidaceae bacterium Broad-1] Length=512 Score = 345 bits (885), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 200/504 (40%), Positives = 295/504 (59%), Gaps = 11/504 (2%) Query 2 KNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLM 61 IG +RA ++P +A+V+ D +TYA+++ L A L A G+A GDRVA L Sbjct 4 SGIGSWPARRARMTPGARAFVQ--DDAVVTYAEVDRLVEAVARGLRARGVAAGDRVAFLG 61 Query 62 PNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRAQ 121 NS+E + A+LGAV +P+NTRLA PE+++IL+D+ ++I+ + A A Sbjct 62 LNSIELAVTLFATARLGAVFLPLNTRLAPPELAWILTDAEPALLIHADDFDEAVSA-EAV 120 Query 122 AD---PPG--TVTDWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVH 176 AD P T G D+LA + A+ VE G DD I YTSGT+G PKGV+ Sbjct 121 ADLGLPSHRFTAAGGHGLDALAVQPAGERAERIDVEVGLDDVFMIQYTSGTSGRPKGVML 180 Query 177 THESVHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIF-SAMRGVTLISMPQFDATK 235 TH ++ A + +D+R + L+ P+FH AAL V+ + ++G T + +FD K Sbjct 181 THGNIAWNAFNLLVDVDIRSDEIALVTAPLFHTAALNQVLLPTFLKGGTSLIAARFDPAK 240 Query 236 VWSLIVEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNI 295 + I ERV + V ++ + Q P +A D R ++GGAP+P L+ + ++ + Sbjct 241 AIATIERERVTLLFGVTSMYQALAQEPTWASADLDSLRSALSGGAPIPRTLLDTWQSRGL 300 Query 296 EVVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGE-GEVVIK 354 ++QGY LTE+ G T+L + D L K GSAG FTDV V DG G+ GEV + Sbjct 301 HIIQGYGLTEASPGTTMLRAGDGLEKLGSAGTPCFFTDVRVVAPDGEDATPGQPGEVHVS 360 Query 355 SDILLKEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEI 414 + YW+ AT FD W RTGD+ +DD+G+LY+ DR+KDM ISGGENVYPAE+ Sbjct 361 GPNVTPGYWHNGAATEAVFDGPWLRTGDVAVLDDDGFLYVVDRVKDMFISGGENVYPAEV 420 Query 415 ESVIIGVPGVSEVAVIGLPDEKWGEIAAAIVVADQNE-VSEQQIVEYCGTRLARYKLPKK 473 E + P V+E AVIG+PDE+WGE+ A+VV + +++ ++++ RLARYK+PK Sbjct 421 EQAVYTHPDVAEAAVIGVPDERWGEVGRAVVVRRPDSALTQDDLLKHLDGRLARYKIPKA 480 Query 474 VIFAEAIPRNPTGKILKTVLREQY 497 V+F + IP N +GK+LK+ +RE Y Sbjct 481 VVFVDEIPHNASGKLLKSRMRELY 504 >gi|345011968|ref|YP_004814322.1| O-succinylbenzoate-CoA ligase [Streptomyces violaceusniger Tu 4113] gi|344038317|gb|AEM84042.1| O-succinylbenzoate-CoA ligase [Streptomyces violaceusniger Tu 4113] Length=508 Score = 343 bits (879), Expect = 5e-92, Method: Compositional matrix adjust. Identities = 202/514 (40%), Positives = 290/514 (57%), Gaps = 22/514 (4%) Query 1 MKNIG---WMLRQRATVSPRLQAYV--EPSTDVRMTYAQMNALANRCADVLTALGIAKGD 55 M+N G W R RA +P A + E STD Y ++ R A L A GI +GD Sbjct 1 MRNEGLGSWPAR-RARKTPHRTALIHGERSTD----YRTLHTRTTRLAHALRARGIGRGD 55 Query 56 RVALLMPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVI 115 R+A L PN + + A LGAV VP+NTRLA PE+++ L+DSG+K +I+G A ++ Sbjct 56 RIAHLGPNHPAYLETLFAAGLLGAVFVPLNTRLATPEIAYQLADSGAKALIHGPAHAGLV 115 Query 116 DAIRAQADPPGTVTDWIGADSLAERLRSAAA---DEPAVECGGDDNLFIMYTSGTTGHPK 172 A+ A G T D+ + L +A+A DEP DD IMYTSGTTG PK Sbjct 116 AALPADT---GVRTCLEIGDAYEQALAAASAEPIDEPVTP---DDICLIMYTSGTTGRPK 169 Query 173 GVVHTHESVHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAM-RGVTLISMPQF 231 G + TH +V A + D+ +R L+ P+FH A L + + +G T + + F Sbjct 170 GAMLTHANVTWNAVNVLVDHDLFADERALVSAPLFHTAGLNMITLPVLLKGGTCVLVEAF 229 Query 232 DATKVWSLIVEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYA 291 D LI R+ VP + + + P ++ D R GG+P+P LI Y Sbjct 230 DPETTLELIERHRITFMFGVPTMFEQVARHPHWSNADLSSLRILTCGGSPVPTPLIAAYQ 289 Query 292 AKNIEVVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGE-GE 350 + + +QGY +TE+ G L +E A KAGSAG F+DV V D + GE GE Sbjct 290 QRGLTFLQGYGMTEAAPGALFLDAEHAAGKAGSAGVPHFFSDVRVVRPDLTPVDTGETGE 349 Query 351 VVIKSDILLKEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVY 410 VV++ ++ YW PE TR F +GWFR+GD +D++GY+YI DR+KDMIISGGEN+Y Sbjct 350 VVVRGPHVMSGYWGLPEETRATFADGWFRSGDAARVDEDGYVYIVDRIKDMIISGGENIY 409 Query 411 PAEIESVIIGVPGVSEVAVIGLPDEKWGEIAAAIVV-ADQNEVSEQQIVEYCGTRLARYK 469 PAEIE +++ PG++E AVIG+PD+ WGE+ A+VV A E+ +++ RLA+YK Sbjct 410 PAEIEDLLLTHPGIAECAVIGVPDDTWGEVPRAVVVPAAGTELDPGEVLASLSGRLAKYK 469 Query 470 LPKKVIFAEAIPRNPTGKILKTVLREQYSATVPK 503 +PK V+ A+ +PR +GK+LK+ +R +Y P+ Sbjct 470 IPKSVVIADELPRTASGKLLKSRVRHRYGTPSPQ 503 >gi|297203702|ref|ZP_06921099.1| O-succinylbenzoate-CoA ligase [Streptomyces sviceus ATCC 29083] gi|197711750|gb|EDY55784.1| O-succinylbenzoate-CoA ligase [Streptomyces sviceus ATCC 29083] Length=501 Score = 341 bits (874), Expect = 2e-91, Method: Compositional matrix adjust. Identities = 192/507 (38%), Positives = 288/507 (57%), Gaps = 14/507 (2%) Query 1 MKNIG---WMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRV 57 M+N G W R RA +P A + D TYA ++ R A L A GI +GDR+ Sbjct 1 MRNEGLGSWPAR-RARKTPHRTALIH--GDTTFTYAALHTRVTRLAHALRARGIRRGDRI 57 Query 58 ALLMPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDA 117 A L PN + + A LGAV VP+NTRLA PE+ + LSDSG++ ++Y A ++ Sbjct 58 AYLGPNHPSYLETLFAAGTLGAVFVPLNTRLAGPEIGYQLSDSGARALVYSPSHAGLVAG 117 Query 118 IRAQADPPGTVTDWIGADSLAERLRSAAADEPAVE-CGGDDNLFIMYTSGTTGHPKGVVH 176 + D V ++ + E+L + EP E DD IMYTSGTTG PKG + Sbjct 118 LPGSTD----VRTYVEVGAEYEQLLADPPAEPIDEPVAPDDTCIIMYTSGTTGRPKGAML 173 Query 177 THESVHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAM-RGVTLISMPQFDATK 235 TH ++ A + D+ +R L+ P+FH A L + + +G T + + FD Sbjct 174 THGNLTWNAINVLVDTDLIADERALVSAPLFHTAGLNMLTLPVLLKGGTCVLVEAFDPNA 233 Query 236 VWSLIVEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNI 295 + LI + R+ VP + + + P +A+ D R GG+P+ LI Y + + Sbjct 234 TFDLIEQHRITFMFGVPTMFEQVARHPRWADADLSSLRILTCGGSPVSTPLIAAYQERGL 293 Query 296 EVVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGE-GEVVIK 354 +QGY +TE+ G L +E A+ KAGSAG F+DV V D + GE GEVV++ Sbjct 294 TFLQGYGMTEASPGTLFLDAEHAISKAGSAGVPHFFSDVRVVRPDLAPVDTGETGEVVLR 353 Query 355 SDILLKEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEI 414 ++ YW PE T +F +GWFR+GD +D++GY++I DR+KDMIISGGEN+YPAEI Sbjct 354 GPHVMPGYWGLPEETAASFADGWFRSGDAARVDEDGYVFIVDRIKDMIISGGENIYPAEI 413 Query 415 ESVIIGVPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSE-QQIVEYCGTRLARYKLPKK 473 E +++G P ++E AVIG+PD+KWGE+ A+VV + V + +++ RLA+YK+PK Sbjct 414 EDLLLGHPDIAECAVIGVPDDKWGEVPRAVVVPREGAVPDPDEVLASLAGRLAKYKIPKS 473 Query 474 VIFAEAIPRNPTGKILKTVLREQYSAT 500 V+ A+ +PR +GK+LK+ +R++Y + Sbjct 474 VVVADELPRTASGKLLKSRVRKRYGSN 500 >gi|9971912|gb|AAG10474.1|AF279106_36 predicted acid--CoA ligase fadD13 [uncultured marine gamma proteobacterium EBAC31A08] Length=504 Score = 338 bits (868), Expect = 1e-90, Method: Compositional matrix adjust. Identities = 192/492 (40%), Positives = 283/492 (58%), Gaps = 7/492 (1%) Query 11 RATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLMPNSVEFCCL 70 +A P A E + TY +++ ++R A L + GI KGDR+A+L N EF L Sbjct 12 QANTRPNKVAIRELDNNKIYTYGELDKRSSRLASHLQSSGIKKGDRIAILSLNCSEFFEL 71 Query 71 FYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRAQADPPGTVT- 129 + K+GA+ +P+N RL PE+S+IL+DS K +IY ++ ++ + + + Sbjct 72 EFACGKIGAIEIPLNWRLTKPELSYILNDSEPKTLIYDNQFEEMVKELKEECNISEIIAL 131 Query 130 DWIGADSLAER-LRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHESVHSAASSW 188 D +S E+ L +A+ E +DN+ IMYTSGTTGHPKG + TH+ + Sbjct 132 DQFDQESDYEKVLSNASGIYYQEEVDLEDNIMIMYTSGTTGHPKGAMITHKMQLFNVINL 191 Query 189 ASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMR-GVTLISMPQFDATKVWSLIVEERVCI 247 + V L+ LP+FH + + G LI + +F+ KV S+I + Sbjct 192 GISAAVSPESVHLVVLPLFHTGGMNCYSNPILHAGGELILLKEFEPGKVLSIIGSSDYGV 251 Query 248 GG--AVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVVQGYALTE 305 AVPA FM P+F + +Y GGAP EA++K Y +K + + QG+ +TE Sbjct 252 THLFAVPAPYQFMMNHPDFESTNLSGVKYAGVGGAPCAEAILKTYISKGVSMQQGWGMTE 311 Query 306 SCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGE-GEVVIKSDILLKEYWN 364 + G T L S +A RK GSAG+ + T+V V GDDG GE GE+ IK + YW Sbjct 312 TSPGATGLESSEAERKIGSAGKPLLHTEVKVVGDDGNELPAGEVGEIYIKGPNITPGYWK 371 Query 365 RPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVIIGVPGV 424 + EATRD+F++GW +TGD DDEG+LYI DR KDM ISGGENVYPAE+++VI +P + Sbjct 372 KEEATRDSFEDGWLKTGDAAYFDDEGFLYIVDRWKDMYISGGENVYPAEVZNVIYQLPQI 431 Query 425 SEVAVIGLPDEKWGEIAAAIV-VADQNEVSEQQIVEYCGTRLARYKLPKKVIFAEAIPRN 483 +EV VIG+ KWGE A V + +E++ ++++++C LA+YK+P+KV F A+PRN Sbjct 432 AEVGVIGIDSPKWGETGKAFVALKPDHELTAEEVIDHCLKNLAKYKIPEKVEFIAALPRN 491 Query 484 PTGKILKTVLRE 495 TGK+LK LR+ Sbjct 492 ATGKVLKRTLRD 503 >gi|311029638|ref|ZP_07707728.1| long-chain-fatty-acid--CoA ligase [Bacillus sp. m3-13] Length=524 Score = 338 bits (867), Expect = 1e-90, Method: Compositional matrix adjust. Identities = 197/505 (40%), Positives = 280/505 (56%), Gaps = 23/505 (4%) Query 16 PRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLMPNSVEFCCLFYGAA 75 P +A + +VR TY + N NR A+ L G+ KGDRV+ L+ N+ E A Sbjct 17 PDKEALYDVKKNVRYTYKEWNVEVNRLANALLKEGVKKGDRVSTLLFNTEELATTMLACA 76 Query 76 KLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRAQADPPGTVTDWI--- 132 K+GAV PIN RL EV+FIL+D+ KVV+Y PVI +I A+ + G WI Sbjct 77 KIGAVFNPINFRLQPEEVAFILTDAKPKVVMYEKALEPVIASIAARFEETGF---WIIDG 133 Query 133 ----GADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHESVHSAASSW 188 + S ERL+SA+ +E + +D IMYTSGTTG PKGVVH H + + Sbjct 134 EQTSYSFSYHERLKSASTEEVTINVLENDTYAIMYTSGTTGRPKGVVHRHRDMAEQSLIV 193 Query 189 ASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMR-GVTLISMPQFDATKVWSLIVEERVCI 247 S + D L+ PMFH A L + + G + + F+ KV LI EE+V Sbjct 194 ISATKLETEDIGLVTAPMFHCAELHCAVIPRIHVGGKNVILHHFEPKKVLQLIQEEKVTK 253 Query 248 GGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIK-IYAAKNIEVVQGYALTES 306 A P + N + Q F E + + G APM AL++ + I +VQ Y +TE Sbjct 254 FFAAPTMWNMLLQ-ENFNEYNLSSLNLGLYGAAPMAPALVRACHDLLGISLVQAYGMTEM 312 Query 307 CGGGTLLLSEDALRKAGSAGRATMFTDVAV--------RGDDGVIREHGEGEVVIKSDIL 358 T L +D LRKAGSAG+A + ++ + D ++ GE+++K + Sbjct 313 GPAITFLSEKDQLRKAGSAGQACLNHEIRIVRPNEDGPSDPDDMLPAGEAGEILVKGPCI 372 Query 359 LKEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVI 418 + Y+NR EAT A GW+ +GDIG +D+EG+LY+KDR+ DMIISGGEN+YP E+E V+ Sbjct 373 MSAYFNREEATAAAMYKGWYHSGDIGYLDEEGFLYVKDRVDDMIISGGENIYPREVEDVL 432 Query 419 IGVPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSEQQIVEYC--GTRLARYKLPKKVIF 476 P V +VA++GLPD++WGE A VV V+E+++ +C + LARYK P+K +F Sbjct 433 YEHPAVLDVAIVGLPDDRWGETVTAFVVKKNENVTEEELDAFCLNSSELARYKRPRKYMF 492 Query 477 AEAIPRNPTGKILKTVLREQYSATV 501 +A+PRN +GKI K VLR+Q V Sbjct 493 VDALPRNASGKIQKFVLRKQMEELV 517 >gi|88319806|emb|CAH10130.1| feruloyl-CoA synthetase [Streptomyces sp. SCC 2136] Length=514 Score = 337 bits (863), Expect = 4e-90, Method: Compositional matrix adjust. Identities = 194/503 (39%), Positives = 281/503 (56%), Gaps = 12/503 (2%) Query 1 MKNIG---WMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRV 57 M+N G W R RA +P A + T V TYA + R A L G+ +GDR+ Sbjct 8 MRNEGLGSWPAR-RARKTPHRTALIHGDTTV--TYAGLYERTTRLAHALRDSGVRRGDRI 64 Query 58 ALLMPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDA 117 A L PN + + A LGAV VP+NTRLA PE+++ L+DSG+K ++YG ++ Sbjct 65 AYLGPNHPSYLETLFAAGTLGAVFVPLNTRLAGPELAYQLTDSGAKALVYGPTFGGLVAG 124 Query 118 IRAQADPPGTVTDWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHT 177 + +D V D L + A DEP + DD IMYTSGTTG PKG + T Sbjct 125 LPGNSDVRTYVEVGAEYDQLLDMASDEAIDEPVIP---DDTCIIMYTSGTTGRPKGAMLT 181 Query 178 HESVHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAM-RGVTLISMPQFDATKV 236 H ++ A + DV +R L+ P+FH A L + + +G T + + FD Sbjct 182 HGNLTWNAVNVLVDQDVITDERALVSAPLFHTAGLNMLTLPVLLKGGTCVLVEAFDPAAT 241 Query 237 WSLIVEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIE 296 + LI R+ VP + + + P +A+ D R GG+P+P LI Y + + Sbjct 242 FDLIERHRITFMFGVPTMFEHVARHPRWADADLSSLRMLSCGGSPVPTPLIAAYQERGLT 301 Query 297 VVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGE-GEVVIKS 355 +QGY +TE+ G L +E A+ KAGSAG F+DV V D + GE GEVV++ Sbjct 302 FLQGYGMTEASPGVLFLDAEHAVTKAGSAGVPHFFSDVRVVRPDLAPADIGETGEVVVRG 361 Query 356 DILLKEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIE 415 ++ YW P+ T F +GWFR+GD ID++GY+ I DRLKDMIISGGEN+YPAEIE Sbjct 362 PHVMPGYWGLPDETAAVFHDGWFRSGDAARIDEDGYVTIVDRLKDMIISGGENIYPAEIE 421 Query 416 SVIIGVPGVSEVAVIGLPDEKWGEIAAAIVVADQN-EVSEQQIVEYCGTRLARYKLPKKV 474 ++ P + E AVIG+PDEKWGE+ A+VV ++ + +++ RLA+YK+PK V Sbjct 422 DQLLAHPDIVECAVIGVPDEKWGEVPRAVVVPREDVALDPDEVLASLAGRLAKYKIPKSV 481 Query 475 IFAEAIPRNPTGKILKTVLREQY 497 + A+ +PR +GK+LK+ +R++Y Sbjct 482 VLADELPRTASGKLLKSRVRKRY 504 >gi|333370466|ref|ZP_08462468.1| long-chain-fatty-acid-CoA ligase [Desmospora sp. 8437] gi|332977814|gb|EGK14571.1| long-chain-fatty-acid-CoA ligase [Desmospora sp. 8437] Length=552 Score = 335 bits (859), Expect = 1e-89, Method: Compositional matrix adjust. Identities = 187/506 (37%), Positives = 286/506 (57%), Gaps = 17/506 (3%) Query 1 MKNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALL 60 M IG +LR RA +SP ++A V S + R+ Y + N + N+ A L I KGDR+A+L Sbjct 44 MAVIGNLLRDRARLSPDMEAIV--SGNRRIRYREYNHMVNQLAHYLLESRIEKGDRIAIL 101 Query 61 MPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYG------APSAPV 114 N ++ AAK+GA+ VP+N R+ E+ +IL D +++ Y P Sbjct 102 CKNQYPMPLIYLAAAKIGAITVPLNWRMKTDELRWILEDCRPRILFYDDDFKQILPLLHE 161 Query 115 IDAIRAQADPPGTVTDWIGADSLAERLRSA-AADEPAVECGGDDNLFIMYTSGTTGHPKG 173 +D + Q V D E L SA EP VE +D I+YTSGTTG PKG Sbjct 162 LDFLEQQI----RVGDGETIHPFFEDLYSARPGGEPKVEVHEEDPALIIYTSGTTGRPKG 217 Query 174 VVHTHESVHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQFDA 233 VV+ H ++++A + +T+D+RY+DR L P+FH++ + ++ MRG TL+ QF Sbjct 218 VVNNHANIYAAGVANTNTLDLRYKDRFLFVTPLFHISGMMFMLAPIMRGFTLVLETQFHP 277 Query 234 TKVWSLIVEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAK 293 K+W L+ E++ +VP ILN+M + + E+D P R + GG+ +P LI++ Sbjct 278 LKIWELLQAEQITGMMSVPVILNYMMEGLKVQEVDVPSLRTILCGGSLVPAPLIRVMFEL 337 Query 294 NIEVVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVA-VRGDDGVIREHGE-GEV 351 VVQ Y TE+ G T + + + S G A ++ + G HGE GE+ Sbjct 338 GYHVVQVYGATETSGAITYWMPDMGIETCNSVGPAIFHAEIKIIDPSTGEALPHGEIGEI 397 Query 352 VIKSDILLKEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYP 411 + +S +L YWNRP+ T NGW+ TGD+G +++ G+L+I DRLKD++I+GGE V+P Sbjct 398 ICRSPVLFAGYWNRPQETEKVLKNGWYHTGDLGWMEENGFLHIVDRLKDVVITGGEKVFP 457 Query 412 AEIESVIIGVPGVSEVAVIGLPDEKWGEIAAAIVVADQNEV-SEQQIVEYCGTRLARYKL 470 A++ESV+ + GV+E AV+G+ E WGE+A A VV + V SE+ ++ + LA + L Sbjct 458 AQVESVLQQLEGVAETAVVGVRHEVWGELARAYVVLKEEAVLSEEDLLAHARKHLADHNL 517 Query 471 PKKVIFAEAIPRNPTGKILKTVLREQ 496 V+F + +P+N GK+LK VLRE Sbjct 518 -HDVVFVKELPKNSMGKVLKFVLREH 542 >gi|68510506|gb|AAY98502.1| putative feruloyl-CoA synthetase [Rhodococcus aetherivorans] Length=511 Score = 332 bits (852), Expect = 7e-89, Method: Compositional matrix adjust. Identities = 206/510 (41%), Positives = 283/510 (56%), Gaps = 18/510 (3%) Query 7 MLRQRATVSPRLQAYVEP---STDVR---MTYAQMNALANRCADVLTALGIAKGDRVALL 60 ML Q P +A P + D R +TY +++ R A L A GI GDRVALL Sbjct 1 MLNQGTGTWPHRRARQAPDAVALDFRGTTVTYRELDERVTRLAHALRARGIGPGDRVALL 60 Query 61 MPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRA 120 N + + + A LGAV VP+N RL PEV + L+DSGS V+I+ A A V DA Sbjct 61 SANHPAYLEVLFAAGLLGAVFVPLNARLTTPEVEYCLADSGSAVLIHSAALADVADAAAT 120 Query 121 QADPPGTVTDWIGADS--------LAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPK 172 A + D E + + AD + +D FIMYTSGTTG PK Sbjct 121 AAGTAAGTARRVVLDGAPDDRAVGYKEIIATGPADRLDLPVTHNDPCFIMYTSGTTGRPK 180 Query 173 GVVHTHESVHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIF-SAMRGVTLISMPQF 231 GVV TH + A +D L P+FH AL + + ++G T+I F Sbjct 181 GVVLTHGNEVFAVMKAIVDLDFLSDQVALDAAPLFHTDALNLIALPTLLKGGTVIVEEGF 240 Query 232 DATKVWSLIVEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYA 291 ++ LI +RV VP +L+ M P + D R +I AP+P ++ Y Sbjct 241 KPGRILHLIETKRVTYSFGVPTMLDAMSTHPNWNRTDLSSIRRWIVAAAPVPPRTLQTYT 300 Query 292 AKNIEVVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGE-GE 350 A+ + + QGY LTE+ G +L +DA RK G+AG FTDV V DG G+ GE Sbjct 301 ARGVRLCQGYGLTETGPGALILTPKDAERKLGTAGAPHFFTDVRVVDPDGPEVAPGDRGE 360 Query 351 VVIKSDILLKEYWNRPEATRDAFD-NGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENV 409 + I +++ YWNRP+AT +AF +GWFR+GD+G D++G++ I DRLKDMIISGGEN+ Sbjct 361 IQISGPNVMRRYWNRPDATAEAFTGDGWFRSGDVGVPDEDGFVTIVDRLKDMIISGGENI 420 Query 410 YPAEIESVIIGVPGVSEVAVIGLPDEKWGEIA-AAIVVADQNEVSEQQIVEYCGTRLARY 468 YPAE+E+ I+ +PGV AV G+PDEKWGE+ AA+ +AD ++E ++ + G RLARY Sbjct 421 YPAEVEAAILDLPGVLGCAVFGVPDEKWGEVGRAAVTLADGATLTEDELFAFLGERLARY 480 Query 469 KLPKKVIFAEAIPRNPTGKILKTVLREQYS 498 K+PK ++ + IPRN TGKI K LREQY+ Sbjct 481 KIPKSLLVLDEIPRNATGKIRKDKLREQYA 510 >gi|316932178|ref|YP_004107160.1| AMP-dependent synthetase and ligase [Rhodopseudomonas palustris DX-1] gi|315599892|gb|ADU42427.1| AMP-dependent synthetase and ligase [Rhodopseudomonas palustris DX-1] Length=508 Score = 332 bits (851), Expect = 9e-89, Method: Compositional matrix adjust. Identities = 199/499 (40%), Positives = 280/499 (57%), Gaps = 10/499 (2%) Query 10 QRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLMPNSVEFCC 69 A V P A V+ +T ++Y M+ +R A L LG+ +GDRVA+L N+VE Sbjct 11 HHAEVRPERIAVVDLATSRNISYRAMDERVDRLAAHLATLGVGRGDRVAVLALNTVETLE 70 Query 70 LFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRAQADPPGTVT 129 + + ++GAV VP+N RL E+S+I+ D+ +V+ + AP+ ++A P + Sbjct 71 VQFACFRIGAVFVPLNVRLTVHELSYIVGDAAPRVLAHDDELAPMAAELKAACGVPHLLA 130 Query 130 DWIGADS-LAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHESVHSAASSW 188 ++ LA R AA EP DD IMYTSGTTG PKG + TH A + Sbjct 131 FGPAYEAALAASPRLGAA-EPVTF---DDVSTIMYTSGTTGKPKGAMITHGMTFINAVNL 186 Query 189 ASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMR-GVTLISMPQFDATKVWSLIVEERVCI 247 I V R L LP+FH L + G T + M FD S+I + V + Sbjct 187 GIPIFVSQRTVFLCVLPLFHTGGLNCYTNPVLHAGGTTLLMRTFDPGAALSIIGDPSVGL 246 Query 248 GG--AVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVVQGYALTE 305 VP+I FM Q P F+ D + GGAPMP L+KI+ + +VQGY +TE Sbjct 247 THFFGVPSIYQFMCQHPAFSATDLSRLQIAGVGGAPMPVPLLKIWQDRGCALVQGYGMTE 306 Query 306 SCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGE-GEVVIKSDILLKEYWN 364 + +L ++DA RKAGSAG+ + D+ + G DG + GE GE+ +K + YWN Sbjct 307 TSPAVLMLDADDAARKAGSAGKPVLHADLKIVGPDGEPVKPGEMGELWVKGPNITPGYWN 366 Query 365 RPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVIIGVPGV 424 RP+A R +F +GW TGD +DDEG+ YI DR KDM ISGGENVYPAE+E V+ +P + Sbjct 367 RPDANRSSFTDGWLHTGDAARVDDEGFYYIVDRTKDMYISGGENVYPAEVEDVLYQLPEI 426 Query 425 SEVAVIGLPDEKWGEIAAAIV-VADQNEVSEQQIVEYCGTRLARYKLPKKVIFAEAIPRN 483 +E AVIG PD +WGE A+V + +E+SE +++ +C RLAR+K P++V F EA+PRN Sbjct 427 AEAAVIGAPDPQWGETGVAVVALKPGHELSEAKLLSHCRERLARFKCPQRVTFVEALPRN 486 Query 484 PTGKILKTVLREQYSATVP 502 TGK+ K LRE+ A P Sbjct 487 ATGKVHKPTLRERILAREP 505 >gi|39933820|ref|NP_946096.1| putative acid-CoA ligase [Rhodopseudomonas palustris CGA009] gi|39647667|emb|CAE26187.1| putative acid-CoA ligase [Rhodopseudomonas palustris CGA009] Length=508 Score = 330 bits (847), Expect = 3e-88, Method: Compositional matrix adjust. Identities = 192/492 (40%), Positives = 274/492 (56%), Gaps = 8/492 (1%) Query 10 QRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLMPNSVEFCC 69 A V P A V+ ++ +++Y M+ +R A L ALG+ +GDRVA+L N+VE Sbjct 11 HHAEVRPERIAVVDLASSRKISYRAMDERVDRLAAHLAALGVGRGDRVAVLALNAVETLE 70 Query 70 LFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRAQADPPGTVT 129 + + +LGA+ VP+N RL E+S+I+ D+ +V+ + AP+ ++A P + Sbjct 71 VQFACFRLGAIFVPLNVRLTVHELSYIVGDAAPRVLAHDDELAPMAKELKAACSVPHLLA 130 Query 130 DWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHESVHSAASSWA 189 ++ A EP DD IMYTSGTTG PKG + TH A + Sbjct 131 FGAAYEAALAASPRLGASEPVTL---DDVSTIMYTSGTTGKPKGAMITHGMTFINAVNLG 187 Query 190 STIDVRYRDRLLLPLPMFHVAALTTVIFSAMR-GVTLISMPQFDATKVWSLIVEERVCIG 248 + R L LP+FH L + G T + M FD S+I + V + Sbjct 188 IPAFISQRTVFLCVLPLFHTGGLNCYTNPVLHAGGTTLLMRAFDPGAALSIIGDPSVGLT 247 Query 249 G--AVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVVQGYALTES 306 VP+I FM Q P FA D + GGAPMP L+KI+ + +VQGY +TE+ Sbjct 248 HFFGVPSIYQFMCQHPAFAATDLSRLQIAGVGGAPMPVPLLKIWQERGCALVQGYGMTET 307 Query 307 CGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGE-GEVVIKSDILLKEYWNR 365 +L ++DA RKAGSAG+ + D+ + G DG + GE GE+ +K + YWNR Sbjct 308 SPAVLMLDADDAARKAGSAGKPVLHADLKIVGPDGDPVKPGEMGELWVKGPNITPGYWNR 367 Query 366 PEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVIIGVPGVS 425 P+A R +F +GW TGD +DDEG+ YI DR KDM ISGGENVYPAE+E V+ +P ++ Sbjct 368 PDANRTSFTDGWLHTGDAARVDDEGFYYIVDRTKDMYISGGENVYPAEVEDVLYQLPEIA 427 Query 426 EVAVIGLPDEKWGEIAAAIV-VADQNEVSEQQIVEYCGTRLARYKLPKKVIFAEAIPRNP 484 E AVIG PD +WGE A+V + E+SE +++ +C RLAR+K P++V F EA+PRN Sbjct 428 EAAVIGAPDPQWGETGVAVVALKPGQELSEAKLLAHCRERLARFKCPQRVSFVEALPRNA 487 Query 485 TGKILKTVLREQ 496 TGK+ K LRE+ Sbjct 488 TGKVHKPTLRER 499 >gi|91978271|ref|YP_570930.1| AMP-dependent synthetase and ligase [Rhodopseudomonas palustris BisB5] gi|91684727|gb|ABE41029.1| AMP-dependent synthetase and ligase [Rhodopseudomonas palustris BisB5] Length=508 Score = 330 bits (845), Expect = 5e-88, Method: Compositional matrix adjust. Identities = 194/502 (39%), Positives = 282/502 (57%), Gaps = 18/502 (3%) Query 8 LRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLMPNSVEF 67 + A V P A ++ +T R+TY ++ +R A L LG+ +GDRVA+L N+ E Sbjct 9 INHHAEVRPDRTALIDLATQRRLTYRALDDRVDRLASHLATLGVGRGDRVAVLAANTTET 68 Query 68 CCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRAQADPPGT 127 + + +LGA+ VP+N RL E++FIL D+ +V+ + SA + ++ + T Sbjct 69 LEVQFACFRLGAIFVPLNIRLTVHELAFILGDATPRVLTHDQDSAVMAHELQTKC----T 124 Query 128 VTDWI--GAD---SLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHESVH 182 + + GA +LA R AA+ + DD IMYTSGTTG PKG + TH Sbjct 125 IAHLLAFGAPYEAALANSPRLGAAEAVTL----DDVSTIMYTSGTTGKPKGAMITHGMTF 180 Query 183 SAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMR-GVTLISMPQFDATKVWSLIV 241 A + V R L LP+FH L + G T+I M FD +I Sbjct 181 INAVNLGIPAFVSQRTVFLCVLPLFHTGGLNCYTNPVLHAGGTVILMRAFDPGAALKIIG 240 Query 242 EERVCIGG--AVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVVQ 299 + + + VP+I FM Q P FAE D + GGA MP L++I+ + +VQ Sbjct 241 DPAIGLTHFFGVPSIYQFMCQHPAFAETDLSRLQLAGVGGASMPVPLLRIWQDRGCALVQ 300 Query 300 GYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGE-GEVVIKSDIL 358 GY +TE+ +L S+DA RKAGSAG+ + D+ + G DG G+ GE+ +K + Sbjct 301 GYGMTETSPAVLMLDSDDAARKAGSAGKPVLHADLKIVGVDGEPVNPGDMGELWVKGPNI 360 Query 359 LKEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVI 418 YWNRP+A R +F +GW TGD +DDEG+ YI DR KDM ISGGENVYPAE+E V+ Sbjct 361 TPGYWNRPDANRTSFTDGWLHTGDAARVDDEGFYYIVDRTKDMYISGGENVYPAEVEDVL 420 Query 419 IGVPGVSEVAVIGLPDEKWGEIAAAIV-VADQNEVSEQQIVEYCGTRLARYKLPKKVIFA 477 +P V+E AVIG PD +WGE AI+ + + +++ E +++ +C RLAR+K P++V+F Sbjct 421 YQLPEVAEAAVIGAPDPQWGETGVAIIAIKNGHDLPETKLLAHCRERLARFKCPQRVVFV 480 Query 478 EAIPRNPTGKILKTVLREQYSA 499 EA+PRN TGK+ K LRE++ A Sbjct 481 EALPRNATGKVHKPTLRERFLA 502 >gi|290956085|ref|YP_003487267.1| substrate-CoA ligase [Streptomyces scabiei 87.22] gi|260645611|emb|CBG68702.1| putative substrate-CoA ligase [Streptomyces scabiei 87.22] Length=503 Score = 329 bits (844), Expect = 5e-88, Method: Compositional matrix adjust. Identities = 195/505 (39%), Positives = 285/505 (57%), Gaps = 15/505 (2%) Query 1 MKNIG---WMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRV 57 M+N G W R RA +P+ A + D +TY +++ R A L A G+ +GDRV Sbjct 1 MRNEGLGSWPAR-RARKTPQRIALIH--GDTSITYGELHERTTRLAHALRAAGVRRGDRV 57 Query 58 ALLMPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDA 117 A L PN + + A LGAV VP+NTRLA PE+++ LSDSG+K ++Y AP Sbjct 58 AYLGPNHPSYLETLFAAGTLGAVFVPLNTRLAGPEIAYQLSDSGAKALVY----APGFTG 113 Query 118 IRAQADPPGT-VTDWIGADSLAERLRSAAADEPAVE-CGGDDNLFIMYTSGTTGHPKGVV 175 + A GT V ++ + E L + A EP + DD IMYTSGTTG PKG + Sbjct 114 LVAGLPGDGTDVRTYLEIGAEYEALLAGAETEPIDQPVTADDTCIIMYTSGTTGRPKGAM 173 Query 176 HTHESVHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAM-RGVTLISMPQFDAT 234 TH ++ A + DV +R L+ P+FH A L + + +G T + + F Sbjct 174 LTHGNIIWNAVNVLVDQDVITDERALVSAPLFHTAGLNMLTLPVLLKGGTCVLVESFVPD 233 Query 235 KVWSLIVEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKN 294 + LI R+ VP + + + + P +AE D R GG+P+P LI + + Sbjct 234 ATFDLIERHRITFMFGVPTMFDQIARHPRWAEADLSSLRMLSCGGSPVPTPLIARFQERG 293 Query 295 IEVVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDV-AVRGDDGVIREHGEGEVVI 353 + +QGY +TE+ G L +E A+ KAGSAG F+DV VR D + GEVV+ Sbjct 294 LTFLQGYGMTEAAPGTLFLDAEHAVSKAGSAGVPHFFSDVRVVRPDMTPVDVDEPGEVVV 353 Query 354 KSDILLKEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAE 413 + ++ YW PE T F +GWFR+GD ID++GY++I DR+KDMIISGGEN+YPAE Sbjct 354 RGPHVMPGYWGLPEETAAVFADGWFRSGDAARIDEDGYVFIVDRIKDMIISGGENIYPAE 413 Query 414 IESVIIGVPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSE-QQIVEYCGTRLARYKLPK 472 IE ++ P + E AVIG+PD+KWGE+ A+VV + V + +++ RLA+YK+PK Sbjct 414 IEDQLLAHPDIVECAVIGVPDDKWGEVPRAVVVPREGCVLDADEVLAALAGRLAKYKIPK 473 Query 473 KVIFAEAIPRNPTGKILKTVLREQY 497 V+ A+ +PR +GK+LK +R++Y Sbjct 474 SVVIADELPRTASGKLLKARVRKRY 498 >gi|192289242|ref|YP_001989847.1| AMP-dependent synthetase and ligase [Rhodopseudomonas palustris TIE-1] gi|192282991|gb|ACE99371.1| AMP-dependent synthetase and ligase [Rhodopseudomonas palustris TIE-1] Length=508 Score = 328 bits (841), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 192/492 (40%), Positives = 273/492 (56%), Gaps = 8/492 (1%) Query 10 QRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLMPNSVEFCC 69 A V P A V+ +T +++Y M+ +R A L ALG+ +GDRVA+L N+VE Sbjct 11 HHAEVRPERIAVVDLATSRKISYRAMDERVDRLAAHLAALGVGRGDRVAVLALNAVETLE 70 Query 70 LFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRAQADPPGTVT 129 + + +LGA+ VP+N RL E+S+I+ D+ +V+ + AP+ ++A P + Sbjct 71 VQFACFRLGAIFVPLNVRLTVHELSYIVGDAAPRVLAHDDELAPMATELKAACSVPHLLA 130 Query 130 DWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHESVHSAASSWA 189 ++ A EP DD IMYTSGTTG PKG + TH A + Sbjct 131 FGPAYEAALAASPLLGASEPVTL---DDVSTIMYTSGTTGKPKGAMITHGMTFINAVNLG 187 Query 190 STIDVRYRDRLLLPLPMFHVAALTTVIFSAMR-GVTLISMPQFDATKVWSLIVEERVCIG 248 + R L LP+FH L + G T + M FD S+I + V + Sbjct 188 IPAFISQRTVFLCVLPLFHTGGLNCYTNPVLHAGGTTLLMRAFDPGAALSIIGDPSVGLT 247 Query 249 G--AVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVVQGYALTES 306 VP+I FM Q FA D + GGAPMP L+KI+ + +VQGY +TE+ Sbjct 248 HFFGVPSIYQFMCQHAAFAATDLLRLQIAGVGGAPMPVPLLKIWQERGCALVQGYGMTET 307 Query 307 CGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGE-GEVVIKSDILLKEYWNR 365 +L ++DA RKAGSAG+ + D+ + G DG + GE GE+ +K + YWNR Sbjct 308 SPAVLMLDADDAARKAGSAGKPVLHADLKIVGPDGAPVKPGEMGELWVKGPNITPGYWNR 367 Query 366 PEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVIIGVPGVS 425 P+A R +F +GW TGD +DDEG+ YI DR KDM ISGGENVYPAE+E V+ +P ++ Sbjct 368 PDANRTSFTDGWLHTGDAARVDDEGFYYIVDRTKDMYISGGENVYPAEVEDVLYQLPEIA 427 Query 426 EVAVIGLPDEKWGEIAAAIV-VADQNEVSEQQIVEYCGTRLARYKLPKKVIFAEAIPRNP 484 E AVIG PD +WGE A+V + E+SE +++ +C RLAR+K P++V F EA+PRN Sbjct 428 EAAVIGAPDPQWGETGVAVVALKPGQELSEAKLLAHCRERLARFKCPQRVSFVEALPRNA 487 Query 485 TGKILKTVLREQ 496 TGK+ K LRE+ Sbjct 488 TGKVHKPTLRER 499 >gi|89098400|ref|ZP_01171284.1| acyl-CoA synthase [Bacillus sp. NRRL B-14911] gi|89086949|gb|EAR66066.1| acyl-CoA synthase [Bacillus sp. NRRL B-14911] Length=519 Score = 328 bits (841), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 204/517 (40%), Positives = 289/517 (56%), Gaps = 25/517 (4%) Query 4 IGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLMPN 63 IG +L P +A + + R TY++ N NR A L A G++KGDRV++ + N Sbjct 5 IGRILDLAVMKFPAKEAVYDVRKNRRYTYSEWNLRINRVAHALAAEGVSKGDRVSVFLYN 64 Query 64 SVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRAQAD 123 + E +F+ AK+GAV PIN RL A E ++IL D+ KV+IY PV+ A+ Q Sbjct 65 TEELAAVFFACAKIGAVFNPINFRLQAEEAAYILRDASPKVLIYEEALEPVVSALEGQF- 123 Query 124 PPGTVTDWIG------ADSLAERLRSAAADEPAV-ECGGDDNLFIMYTSGTTGHPKGVVH 176 P T +I A S E++ S A+D P + DD+ IMYTSGTTG PKGV+H Sbjct 124 -PATAFWYIDEEPPSYAASFDEKV-SQASDSPVFADVSEDDDYAIMYTSGTTGRPKGVIH 181 Query 177 THESVHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMR-GVTLISMPQFDATK 235 TH + + + + + D+ L+ PMFH A L + G I + QF+ K Sbjct 182 THRHMAEQSLTVIAATKLEPSDKGLVTAPMFHCAELHCAFLPRVHVGAGNIILHQFNPKK 241 Query 236 VWSLIVEERVCIGGAVPAILNFMRQVPEFAE-LDAPDFRYFITGGAPMPEALIKIYAAK- 293 V LI EE + A P + N + Q A LD+ + + G APM AL+K+ K Sbjct 242 VLQLIEEEGITKFFAAPTMWNMILQEDLMAYCLDS--LKLGLYGAAPMAPALVKLCREKL 299 Query 294 NIEVVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVA-VRGDDG------VIREH 346 I +VQ Y +TE T L D L KAGSAG+A + ++ VR +D I Sbjct 300 GILLVQAYGMTEMGPAITFLSEHDQLSKAGSAGQACLNHEIRIVRPNDNGPSNPEDILPA 359 Query 347 GE-GEVVIKSDILLKEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISG 405 GE GE+++K ++K Y+N E T A GW+ +GDIG +D++GYL+++DR+ DMIISG Sbjct 360 GEAGEIIVKGPSMMKGYYNMKETTEKALYKGWYHSGDIGALDEDGYLWVRDRVDDMIISG 419 Query 406 GENVYPAEIESVIIGVPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSEQQIVEYC--GT 463 GEN+YP EIE V+ GV + AVIG PD++WGE A VV VSE+++ +C + Sbjct 420 GENIYPREIEDVLFEHSGVLDAAVIGQPDDRWGETVTAFVVKKDPSVSEEELDSWCKESS 479 Query 464 RLARYKLPKKVIFAEAIPRNPTGKILKTVLREQYSAT 500 RLA YK P+K IF E++PRN +GK+ K VLR + S + Sbjct 480 RLANYKRPRKYIFCESLPRNASGKLQKFVLRSELSQS 516 >gi|288553808|ref|YP_003425743.1| AMP-dependent synthetase and ligase [Bacillus pseudofirmus OF4] gi|288544968|gb|ADC48851.1| AMP-dependent synthetase and ligase [Bacillus pseudofirmus OF4] Length=491 Score = 328 bits (840), Expect = 2e-87, Method: Compositional matrix adjust. Identities = 183/495 (37%), Positives = 268/495 (55%), Gaps = 22/495 (4%) Query 6 WMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLMPNSV 65 W+ + RA ++P A ++ ST R TY ++N A R A +L GI KGDR+ALL PN + Sbjct 7 WLCK-RAQLTPWNIAIIDGSTKERWTYQELNERAERLAGMLQQRGIKKGDRIALLSPNHI 65 Query 66 EFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRAQADPP 125 + L + A KLGA+ VP+N RL++ E+++I SD +V ++D Sbjct 66 SYFDLLFAATKLGAIFVPLNWRLSSTELAYIFSDCEPSLVFVDEIFISLVDGGVPSVQLN 125 Query 126 GTVTDWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHESVH--- 182 +WI + AA E D L ++YT GTTG PKGVV TH+SV Sbjct 126 QEYKEWI----------AKAAPCKKEEISDSDPLLLIYTGGTTGLPKGVVLTHQSVFFNM 175 Query 183 -SAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMR-GVTLISMPQFDATKVWSLI 240 + +SW T D D+ + LPMFH L + + G T+I QFD K LI Sbjct 176 INTITSWGLTSD----DKTITSLPMFHTGGLNALSLPILHIGGTVILSDQFDGEKTIQLI 231 Query 241 VEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVVQG 300 E + + VP + + + + P F E F++GGAP + K + K + +G Sbjct 232 EEFQCTVILLVPTMYHLLIKAPSFKEATFRSVHTFLSGGAPCSYVIYKAFQKKGLAFKEG 291 Query 301 YALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGE-GEVVIKSDILL 359 Y LTE+ + +A+ K GS G+ ++ +V + ++G +H E GE+ I+ + Sbjct 292 YGLTEAGPNNFFIDPSEAVSKRGSVGKPMLYNEVKIIKENGEEADHDEVGEICIRGLHVF 351 Query 360 KEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVII 419 YW +PE T + NGW TGD+G D EGY YI R KDMII+GGENVYP E+E V+ Sbjct 352 DSYWQKPEITTNTVINGWLHTGDLGRRDKEGYFYIVGRKKDMIITGGENVYPLEVEHVLN 411 Query 420 GVPGVSEVAVIGLPDEKWGEIAAAIVVADQN-EVSEQQIVEYCGTRLARYKLPKKVIFAE 478 P VSE AV+GL DEKWGE+ +A +V +N +SE++++ +C L RYK+PK++ F E Sbjct 412 EHPAVSEAAVVGLADEKWGEVVSAFIVVKKNFTLSEEELLAHCQRTLGRYKIPKRITFVE 471 Query 479 AIPRNPTGKILKTVL 493 +P+ P GK+ K L Sbjct 472 ELPKTPVGKVDKKKL 486 >gi|159900315|ref|YP_001546562.1| AMP-dependent synthetase and ligase [Herpetosiphon aurantiacus DSM 785] gi|159893354|gb|ABX06434.1| AMP-dependent synthetase and ligase [Herpetosiphon aurantiacus DSM 785] Length=499 Score = 327 bits (837), Expect = 4e-87, Method: Compositional matrix adjust. Identities = 183/499 (37%), Positives = 271/499 (55%), Gaps = 9/499 (1%) Query 4 IGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTA-LGIAKGDRVALLMP 62 IG L +R ++P A V+ R +Y Q+N+ ANR A L G+ KGDRVA+L Sbjct 3 IGDWLGKRELLTPERLALVDDRDGERYSYRQLNSRANRLAASLRQRFGVGKGDRVAILAK 62 Query 63 NSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRAQA 122 N + + + KLGA+ VP+N RL E+ ++L DS S +++Y + AP++ +R+Q Sbjct 63 NQIGYLDALFATGKLGAILVPLNWRLTEHELIYMLKDSASSILLYDSQFAPLLPTLRSQT 122 Query 123 DPPGTVTDWIGADSLAERLRSAAADEPAVECGG-DDNLFIMYTSGTTGHPKGVVHTHESV 181 + + ++L + A+D P E DD I+YTSGTTG PKG V +H + Sbjct 123 ----PIKQCVQFGPEYDQLLTQASDLPISESVDLDDPHLILYTSGTTGAPKGAVLSHRVL 178 Query 182 HSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMR-GVTLISMPQFDATKVWSLI 240 + + D+ D ++ P+FH L + + G T++ M +++ + LI Sbjct 179 VWNSLNTNVGWDLHADDVSIIHTPLFHTGGLNVLTLPILHAGGTMVLMQEWNPERCLQLI 238 Query 241 VEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVVQG 300 +E V I AVP + + Q P F + + R+ I GG+P P LI+ Y +NI QG Sbjct 239 EQEHVTIFFAVPTMFEMLLQAPNFVQTNLSSLRFCIAGGSPCPIPLIEAYQQRNIPFRQG 298 Query 301 YALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGV-IREHGEGEVVIKSDILL 359 Y LTE L EDA+RKAGS G+ D + + G + + GE+++ + Sbjct 299 YGLTEVSVNCFTLNPEDAIRKAGSVGKPIFHLDARIVDEAGRDVPTNSIGELILYGPTVC 358 Query 360 KEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVII 419 YW P AT A GWF TGD+ +D EGY YI DR KDM ISGGENVYPAE+E+V+ Sbjct 359 NGYWRNPVATAQALQKGWFYTGDLARVDAEGYFYIVDRKKDMYISGGENVYPAEVENVLY 418 Query 420 GVPGVSEVAVIGLPDEKWGEIAAAIVVAD-QNEVSEQQIVEYCGTRLARYKLPKKVIFAE 478 P V E AVIG+PD +WGE+ A+VV ++ E ++ +C RLA YK PK + F Sbjct 419 QHPAVQECAVIGIPDSRWGEVGRALVVLRPSTQLDEPTLIAFCRERLASYKTPKSIYFLP 478 Query 479 AIPRNPTGKILKTVLREQY 497 +P N +GK++K LR+ + Sbjct 479 ELPHNASGKVVKPELRKLF 497 >gi|119963319|ref|YP_946756.1| O-succinylbenzoate-CoA ligase [Arthrobacter aurescens TC1] gi|119950178|gb|ABM09089.1| O-succinylbenzoate-CoA ligase [Arthrobacter aurescens TC1] Length=516 Score = 327 bits (837), Expect = 4e-87, Method: Compositional matrix adjust. Identities = 197/519 (38%), Positives = 285/519 (55%), Gaps = 25/519 (4%) Query 1 MKNIG---WMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRV 57 M N G W+ R+R + T +TYA+++ +R A+ L G+AKGDRV Sbjct 1 MDNNGVGSWLHRRRIKSGTKAAVISGGHT---VTYAELSDRTDRLANALKGRGVAKGDRV 57 Query 58 ALLMPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVI-- 115 A L N F F+ LGA+ VP+NTRLAAPE+ F L DSG++++I GA + VI Sbjct 58 AYLGENHPSFVETFFACGLLGAIFVPLNTRLAAPELQFQLQDSGARLLI-GAATLEVIAA 116 Query 116 ------DAIRAQADPPGTVTDWI------GADSLAERLRSAAADEPAVECGGDDNLFIMY 163 D P TD G + E L +A A DD I+Y Sbjct 117 AAVADTDVSHRLVVAPDDGTDGSVVKLPSGVEHYGEVLDAAPATPLDESVTLDDAAMILY 176 Query 164 TSGTTGHPKGVVHTHESVHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAM-RG 222 TSGTTG PKG + TH ++ + +D+ D L+ P+FHVA+L + + +G Sbjct 177 TSGTTGKPKGALLTHGNITWNCINTVVDMDLSRNDVALMISPLFHVASLDMGLLPMLLKG 236 Query 223 VTLISMPQFDATKVWSLIVEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPM 282 T++ +FDA + L+ + +V VP + P ++ D GG+ + Sbjct 237 ATVVLESKFDAGRALQLVAQHKVTTLNGVPTTFQMLCDHPGWSTADLTSLEKLTCGGSAV 296 Query 283 PEALIKIYAAKNIEVVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGV 342 P +++ Y + I Y +TE+ G T+L + KAGSAG FTDV + G Sbjct 297 PGRVLEAYEQRGIGFTSCYGMTETAPGTTMLPVSRSKEKAGSAGLPHFFTDVRIAEPLGG 356 Query 343 IREHGE-GEVVIKSDILLKEYWNRPEATRDAF-DNGWFRTGDIGEIDDEGYLYIKDRLKD 400 I GE GE+ I ++KEYWNRPEAT +++ D+ WFR+GD+G D +G+L++ DR+KD Sbjct 357 IAALGEVGEIQISGPNVIKEYWNRPEATAESYSDSSWFRSGDMGYQDGDGFLFVSDRIKD 416 Query 401 MIISGGENVYPAEIESVIIGVPGVSEVAVIGLPDEKWGEIAAAIVVADQN-EVSEQQIVE 459 MIISGGEN+YPAE+E+ I +P V VAVIG+ D+KWGE+ AIV +N ++E+Q+ Sbjct 417 MIISGGENIYPAEVEAAIAELPAVGSVAVIGVDDQKWGEVPRAIVTLRENASLTEEQLRS 476 Query 460 YCGTRLARYKLPKKVIFAEAIPRNPTGKILKTVLREQYS 498 + RLARYK+PK V+F E +PR +GKI K LR+QY+ Sbjct 477 HLEGRLARYKIPKSVVFVEEMPRTASGKIRKMDLRKQYA 515 >gi|138894249|ref|YP_001124702.1| long-chain-fatty-acid--CoA ligase [Geobacillus thermodenitrificans NG80-2] gi|134265762|gb|ABO65957.1| Long-chain-fatty-acid-CoA ligase, putative [Geobacillus thermodenitrificans NG80-2] Length=516 Score = 324 bits (831), Expect = 2e-86, Method: Compositional matrix adjust. Identities = 200/522 (39%), Positives = 288/522 (56%), Gaps = 31/522 (5%) Query 1 MKNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALL 60 M N+ L Q A P AYV R TY Q+NA ++ AD L LGI +GD VALL Sbjct 2 MMNLTARLAQMAKQLPDKDAYV--FMGERCTYRQLNAAISQFADGLAQLGIRQGDHVALL 59 Query 61 MPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRA 120 + NS +F YGA +LGA +PIN E+S+IL + K I AP+ + Sbjct 60 LGNSPQFVVSLYGALRLGATVIPINPIYTPEEISYILHNGDVKAAIGLDLLAPLFAEAKK 119 Query 121 Q---------ADPPGTVTDWIG----ADSLAERLRSAAADEPAVECGGDDNLFIMYTSGT 167 + + P I S AE L + + D E DD I+YTSGT Sbjct 120 RLPLLKYAIVCETPQGKEKGISLSGEMKSFAEVLAAGSPDFTGPELKDDDVAVILYTSGT 179 Query 168 TGHPKGVVHTHESVHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSA-MRGVTLI 226 TG PKG + TH++++S A A + + DR++ LPMFHV LT + + M G T++ Sbjct 180 TGKPKGAMLTHKNLYSNAQDTADYLGINENDRVIAALPMFHVFCLTVALNAPLMNGGTVL 239 Query 227 SMPQFDATKVWSLIVEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEAL 286 MP+F +++++L EE+ I VP + NF+ Q E + D R I+GGA MP AL Sbjct 240 IMPKFSPSQLFALAREEKATIFAGVPTMYNFLYQHEEGSADDLRTLRLCISGGASMPVAL 299 Query 287 IKIYAAK-NIEVVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIRE 345 ++ + K N+ V +GY L+E+ T D RK GS G T +V ++ V+ E Sbjct 300 LENFEKKFNVIVSEGYGLSEASPV-TCFNPLDRPRKPGSIG--TSIKNV----ENKVVNE 352 Query 346 HGE-------GEVVIKSDILLKEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRL 398 +GE GE+V++ ++K Y+ PE T A +GW TGD+ +D++GY YI DR Sbjct 353 YGEEVPIGEVGELVVRGPNVMKGYYKMPEETAAALRDGWLHTGDLARMDEDGYFYIVDRK 412 Query 399 KDMIISGGENVYPAEIESVIIGVPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSEQQIV 458 K+MII GG NVYP E+E V+ P V E AVIG+PD +GE+ A VV E++E+Q++ Sbjct 413 KEMIIVGGYNVYPREVEEVLYSHPDVVEAAVIGVPDPDYGEMVRAFVVTKNPELTEEQLL 472 Query 459 EYCGTRLARYKLPKKVIFAEAIPRNPTGKILKTVLREQYSAT 500 YC RLA+YK+P ++ F E +P+N TGKIL+ L+E+ +A+ Sbjct 473 AYCRERLAKYKVPGEIDFLEELPKNATGKILRRALKERLTAS 514 >gi|196248155|ref|ZP_03146857.1| AMP-dependent synthetase and ligase [Geobacillus sp. G11MC16] gi|196212939|gb|EDY07696.1| AMP-dependent synthetase and ligase [Geobacillus sp. G11MC16] Length=514 Score = 323 bits (828), Expect = 5e-86, Method: Compositional matrix adjust. Identities = 199/520 (39%), Positives = 287/520 (56%), Gaps = 31/520 (5%) Query 3 NIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLMP 62 N+ L Q A P AYV R TY Q+NA ++ AD L LGI +GD VALL+ Sbjct 2 NLTARLAQMAKQLPDKDAYV--FMGERCTYRQLNAAISQFADGLAQLGIRQGDHVALLLG 59 Query 63 NSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRAQ- 121 NS +F YGA +LGA +PIN E+S+IL + K I AP+ + + Sbjct 60 NSPQFVVSLYGALRLGATVIPINPIYTPEEISYILHNGDVKAAIGLDLLAPLFAEAKKRL 119 Query 122 --------ADPPGTVTDWIG----ADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTG 169 + P I S AE L + + D E DD I+YTSGTTG Sbjct 120 PLLKYAIVCETPQGKEKGISLSGEMKSFAEVLAAGSPDFTGPELKDDDVAVILYTSGTTG 179 Query 170 HPKGVVHTHESVHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSA-MRGVTLISM 228 PKG + TH++++S A A + + DR++ LPMFHV LT + + M G T++ M Sbjct 180 KPKGAMLTHKNLYSNAQDTADYLGINENDRVIAALPMFHVFCLTVALNAPLMNGGTVLIM 239 Query 229 PQFDATKVWSLIVEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIK 288 P+F +++++L EE+ I VP + NF+ Q E + D R I+GGA MP AL++ Sbjct 240 PKFSPSQLFALAREEKATIFAGVPTMYNFLYQHEEGSADDLRTLRLCISGGASMPVALLE 299 Query 289 IYAAK-NIEVVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHG 347 + K N+ V +GY L+E+ T D RK GS G T +V ++ V+ E+G Sbjct 300 NFEKKFNVIVSEGYGLSEASPV-TCFNPLDRPRKPGSIG--TSIKNV----ENKVVNEYG 352 Query 348 E-------GEVVIKSDILLKEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKD 400 E GE+V++ ++K Y+ PE T A +GW TGD+ +D++GY YI DR K+ Sbjct 353 EEVPIGEVGELVVRGPNIMKGYYKMPEETAAALRDGWLHTGDLARMDEDGYFYIVDRKKE 412 Query 401 MIISGGENVYPAEIESVIIGVPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSEQQIVEY 460 MII GG NVYP E+E V+ P V E AVIG+PD +GE+ A VV E++E+Q++ Y Sbjct 413 MIIVGGYNVYPREVEEVLYSHPDVVEAAVIGVPDPDYGEMVRAFVVTKNPELTEEQLLAY 472 Query 461 CGTRLARYKLPKKVIFAEAIPRNPTGKILKTVLREQYSAT 500 C RLA+YK+P ++ F E +P+N TGKIL+ L+E+ +A+ Sbjct 473 CRERLAKYKVPGEINFLEELPKNATGKILRRALKERLTAS 512 >gi|90417333|ref|ZP_01225259.1| putative fatty-acid--coa ligase transmembrane protein [marine gamma proteobacterium HTCC2207] gi|90330918|gb|EAS46181.1| putative fatty-acid--coa ligase transmembrane protein [marine gamma proteobacterium HTCC2207] Length=508 Score = 323 bits (827), Expect = 5e-86, Method: Compositional matrix adjust. Identities = 188/504 (38%), Positives = 283/504 (57%), Gaps = 18/504 (3%) Query 4 IGWMLRQRATVSPRLQAYV-EPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLMP 62 +G+ + +RA +SP +A E T T+ + + A +L A G+ +GDRV + Sbjct 9 LGYTILRRAALSPERKALTFEGKTS---TFGEFGQRIRKLAALLRAGGVCRGDRVGYIGL 65 Query 63 NSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRAQA 122 N F Y + LGA+ VP+N RL PE+ +I +D+G ++ ++D R Sbjct 66 NHPAFLEALYACSCLGAIFVPLNFRLTGPEMRYITNDAGIHTMLADNGLRSLVDQERDNL 125 Query 123 DPPGTVT------DWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVH 176 ++ +W +SL L+ + E + D+ FIMYTSGTTG PKG + Sbjct 126 VCQRYISIEDKAANW---ESLESLLQDSQPLELIEQVDADEVAFIMYTSGTTGLPKGAML 182 Query 177 THESVHSAASSWASTIDVRYRDRLLLPLPMFHVAALT-TVIFSAMRGVTLISMPQFDATK 235 TH ++ +++ A D L P+FH+ L T + S RG+ ++ + FDA + Sbjct 183 THGNLFWNSANTAYGEDFMGTTTLTCA-PLFHIGGLNVTTLPSLARGIEVVLIRSFDAAE 241 Query 236 VWSLIVEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNI 295 V + + + +V P + M Q FA D F+ GGAP+P LI +Y+ +++ Sbjct 242 VLAALEKYQVSTMFGAPTMFLMMSQHDSFASTDLSHIGAFVVGGAPVPVPLINLYSDRDV 301 Query 296 EVVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVA-VRGDDGVIREHGEGEVVIK 354 QGY LTE+ T+L + AL K GSAGR MFTDV V G++ + GEV ++ Sbjct 302 RFCQGYGLTETSPFATMLETSMALEKVGSAGRPPMFTDVRIVDGENNPLPAGEHGEVCVR 361 Query 355 SDILLKEYWNRPEATRDAFD-NGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAE 413 +LK YWNRPEAT DA D GWF +GDIG D++GYL++ DR+KDM+ISGGEN+YPAE Sbjct 362 GPNVLKGYWNRPEATADAIDAQGWFHSGDIGYFDNQGYLFLCDRVKDMVISGGENIYPAE 421 Query 414 IESVIIGVPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSE-QQIVEYCGTRLARYKLPK 472 +ES++ G V EVAVIGLPDEKWGE A+ V Q+ + +++ E+ LA+YKLP Sbjct 422 VESILYGHSSVVEVAVIGLPDEKWGEAVTAVAVLKQDATLDLEELREFASQSLAKYKLPS 481 Query 473 KVIFAEAIPRNPTGKILKTVLREQ 496 ++ F + +PRNP GK+ K ++++ Sbjct 482 RLYFLDELPRNPAGKVQKFKIKDE 505 >gi|90416827|ref|ZP_01224757.1| acyl-CoA synthetase [marine gamma proteobacterium HTCC2207] gi|90331580|gb|EAS46816.1| acyl-CoA synthetase [marine gamma proteobacterium HTCC2207] Length=512 Score = 323 bits (827), Expect = 6e-86, Method: Compositional matrix adjust. Identities = 188/483 (39%), Positives = 276/483 (58%), Gaps = 18/483 (3%) Query 31 TYAQMNALANRCADVLTALGIAKGDRVALLMPNSVEFCCLFYGAAKLGAVAVPINTRLAA 90 TYA ++ + A VL+ LG+++GDRV + N F Y + LGA+ VP+N RL Sbjct 36 TYADLSDRIRKLAAVLSQLGVSRGDRVGYMGLNHPCFLEAVYACSCLGAIFVPLNFRLTP 95 Query 91 PEVSFILSDSGSKVVIYGAPSAPVIDAIRAQADPPGTV---TDWIGADSLAERLRSAAAD 147 E FI+ DSG ++V+ ++D + + + +D G SL L +AA Sbjct 96 SEAGFIIDDSGIQIVLADDACTAILDKQKQELSVQHYLAIESDRPGWQSLENLLATAAPI 155 Query 148 EPAVECGGDDNLFIMYTSGTTGHPKGVVHTHESVHSAASSWASTIDVRYRDRLLLPL--- 204 E DD IMYTSGTTG PKG + TH ++ W +T++V + ++ Sbjct 156 ETVTAIEADDVALIMYTSGTTGLPKGAMLTHGNIF-----W-NTVNVSLLEESMVGASLT 209 Query 205 --PMFHVAALT-TVIFSAMRGVTLISMPQFDATKVWSLIVEERVCIGGAVPAILNFMRQV 261 P+FH+ L T S +RGV ++ FDA V I + +V P + M Q Sbjct 210 CAPLFHIGGLNVTTHISLVRGVAVVLHRSFDAGAVLHDIEKYQVSTMFGAPTMFTMMSQH 269 Query 262 PEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVVQGYALTESCGGGTLLLSEDALRK 321 FA D F G AP+P L+ IYA++ + QGY LTE+ T+L S+ A K Sbjct 270 EAFASTDFSSVISFNVGSAPVPLPLLNIYASRGVTFCQGYGLTETSPYVTVLGSKFATSK 329 Query 322 AGSAGRATMFTDVAVRGDDGVIREHGE-GEVVIKSDILLKEYWNRPEATRDAFD-NGWFR 379 GSAG++ MFT V + G +GE GE+ IK ++K YWNRPEAT +A D +GWF Sbjct 330 IGSAGQSLMFTSVRIVDGRGQTVANGERGEIWIKGPNVMKGYWNRPEATAEAVDEDGWFH 389 Query 380 TGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVIIGVPGVSEVAVIGLPDEKWGE 439 +GD+G DD+ +L+I DR+KDM+ISGGEN+YPAE+ESV+ ++EVAVIG+PD+KWGE Sbjct 390 SGDVGYFDDDNFLFICDRIKDMVISGGENIYPAEVESVLFEHSAIAEVAVIGVPDDKWGE 449 Query 440 IAAAIVVADQNEVSE-QQIVEYCGTRLARYKLPKKVIFAEAIPRNPTGKILKTVLREQYS 498 + A+VV + + +++ + G +LARYKLP+K+ +A+PRNP GK+ K +L++Q + Sbjct 450 LLVAVVVLHEGTTLDLEELQGFVGGKLARYKLPRKLHLVDALPRNPAGKVKKFILKQQMT 509 Query 499 ATV 501 V Sbjct 510 EAV 512 >gi|301061414|ref|ZP_07202184.1| AMP-binding enzyme [delta proteobacterium NaphS2] gi|300444453|gb|EFK08448.1| AMP-binding enzyme [delta proteobacterium NaphS2] Length=510 Score = 322 bits (825), Expect = 9e-86, Method: Compositional matrix adjust. Identities = 196/513 (39%), Positives = 283/513 (56%), Gaps = 31/513 (6%) Query 4 IGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLMPN 63 IG L +RA ++P +A V+ + R TY +N NR A L LG+ GDR+++L N Sbjct 7 IGSWLTKRAVLTPDSEAVVD--GERRFTYRALNGRVNRLARALQGLGVKSGDRLSILSTN 64 Query 64 SVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRAQAD 123 VE+ AKL + VP+N RL E++F+++DSG++++I+ + DA D Sbjct 65 CVEYVETIMATAKLSVMLVPLNWRLTPTELAFMVNDSGARILIF---HPDLSDAAVQLKD 121 Query 124 PPGTVTDWI------GADSLAERLRSAAADE---PAVECGGDDNLFIMYTSGTTGHPKGV 174 G + G E L A +E P D + IMYT+GTTG PKG Sbjct 122 KTGLEKLLVLRFEGNGHGDAYELLLKAQGNEEPIPDTHPDLDTSHIIMYTAGTTGQPKGA 181 Query 175 VHTHESVHSAASSWAS---TIDVRYR--DRLLLPLPMFHVAALTTVIFSAM-RGVTLISM 228 V + AS W + T+D+ + DR L LPMFH+ + + G T++ Sbjct 182 V-----LSQGASFWNAVNLTVDMGFSSADRNLAVLPMFHIGGIGLFTLPMLYMGGTVVIQ 236 Query 229 PQFDATKVWSLIVEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIK 288 F+ + L+ E + + V AI F+ Q P F R ++GGAP+P +L+ Sbjct 237 KTFEPERTLELLETENITLFFGVAAIFLFLIQHPNFKREAFDKVRVVMSGGAPLPVSLVH 296 Query 289 IYAAKNIEVVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAV---RGDDGVIRE 345 YA K+I + QG+ ++E+ L A++KAGS GRA M + V R D + Sbjct 297 EYADKSIILQQGFGMSEAAPSIATLEKGLAIKKAGSIGRALMHVEARVVDARMKD--VPT 354 Query 346 HGEGEVVIKSDILLKEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISG 405 GE+VI+ LLKEYWNRP+AT++AF GWF TGD+ +D +G L I +R KDM ISG Sbjct 355 DAVGELVIRGPNLLKEYWNRPDATKEAFAGGWFHTGDLARMDPDGDLTIVERKKDMFISG 414 Query 406 GENVYPAEIESVIIGVPGVSEVAVIGLPDEKWGEIAAAIVVADQNE-VSEQQIVEYCGTR 464 GENVYPAE+E I +P +SE AVIG+ DEKWGE+ A+VV + E ++E ++ + R Sbjct 415 GENVYPAEVEDAIYQLPQISETAVIGIKDEKWGEVGKAVVVLKKGEMLTEDAVLTHLKER 474 Query 465 LARYKLPKKVIFAEAIPRNPTGKILKTVLREQY 497 LA+YK+PK V+F +PRN GK+LK VLRE + Sbjct 475 LAKYKIPKSVVFTGPLPRNAAGKVLKNVLREHH 507 >gi|86751180|ref|YP_487676.1| AMP-dependent synthetase and ligase [Rhodopseudomonas palustris HaA2] gi|86574208|gb|ABD08765.1| AMP-dependent synthetase and ligase [Rhodopseudomonas palustris HaA2] Length=508 Score = 322 bits (825), Expect = 1e-85, Method: Compositional matrix adjust. Identities = 190/498 (39%), Positives = 277/498 (56%), Gaps = 14/498 (2%) Query 8 LRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLMPNSVEF 67 + A V P A ++ +T R+TY ++ +R A L +LG+ +GDRVA+L N+ + Sbjct 9 ITHHAEVRPDRIALIDLATQRRLTYRALDDRIDRLASHLASLGVGRGDRVAVLAANTTDT 68 Query 68 CCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRAQADPPGT 127 + + +LGA+ VP+N RL E+SFI+ D+ V+ + + A + ++++ Sbjct 69 LEVQFACFRLGAIFVPLNVRLTVHELSFIVGDAAPLVLAHDSDFAAMAGDLQSKCGIAHL 128 Query 128 V---TDWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHESVHSA 184 + + A S A RL A A DD IMYTSGTTG PKG + TH Sbjct 129 LQFGAPYEAALSAAPRLARAEA------VTLDDVSTIMYTSGTTGKPKGAMITHLMTFIN 182 Query 185 ASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMR-GVTLISMPQFDATKVWSLIVEE 243 A + + R L +P+FH L + G T+I M FD + LI + Sbjct 183 AVNLGIPAFITQRTVFLCVMPLFHTGGLNCYTNPVLHAGGTVILMRAFDPGETLRLIGDA 242 Query 244 RVCIGG--AVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVVQGY 301 + + VP+I FM Q P FA+ D + GGAPMP L++I+ + +VQGY Sbjct 243 GIGLTHFFGVPSIYQFMCQHPAFADTDLSRLQMAGVGGAPMPVPLLRIWQERGCTLVQGY 302 Query 302 ALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGE-GEVVIKSDILLK 360 +TE+ +L S+DA RKAGSAG+ + D+ + G DG GE GE+ +K + Sbjct 303 GMTETSPAVMMLDSDDAARKAGSAGKPVLHADLKIVGPDGEPVAPGEMGELWVKGPNITP 362 Query 361 EYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVIIG 420 YWNRPEA R +F +GW TGD +D EG+ YI DR KDM ISGGENVYPAE+E V+ Sbjct 363 GYWNRPEANRTSFTDGWLHTGDAARVDGEGFYYIVDRTKDMYISGGENVYPAEVEDVLYQ 422 Query 421 VPGVSEVAVIGLPDEKWGEIAAAIV-VADQNEVSEQQIVEYCGTRLARYKLPKKVIFAEA 479 +P ++E AVIG PD +WGE AI+ V +++ E ++ +C RLAR+K P++V+F EA Sbjct 423 LPEIAEAAVIGAPDPQWGETGVAIIAVKTGHDLPEAKLHTHCRERLARFKCPQRVVFVEA 482 Query 480 IPRNPTGKILKTVLREQY 497 +PRN TGK+ K LRE++ Sbjct 483 LPRNATGKVHKPTLRERF 500 >gi|297531137|ref|YP_003672412.1| O-succinylbenzoate-CoA ligase [Geobacillus sp. C56-T3] gi|297254389|gb|ADI27835.1| O-succinylbenzoate-CoA ligase [Geobacillus sp. C56-T3] Length=514 Score = 322 bits (824), Expect = 1e-85, Method: Compositional matrix adjust. Identities = 195/520 (38%), Positives = 284/520 (55%), Gaps = 31/520 (5%) Query 3 NIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLMP 62 N+ L Q A P +AYV R TY Q++ +R AD L LG+ +GD +ALL+ Sbjct 2 NVTTRLAQIAKQLPDKEAYV--FMKERCTYQQLDEAISRFADGLARLGVRRGDHIALLLG 59 Query 63 NSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIY----------GAPSA 112 NS +F YGA +LGA +PIN E+S+IL + K VI Sbjct 60 NSPQFVIGLYGALRLGATVIPINPIYTPEEISYILHNGDVKAVISLDLLAPLFVEAKKRL 119 Query 113 PVIDAIRAQADPPGT---VTDWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTG 169 P+++ P G ++ G S AE L + D E G DD I+YTSGTTG Sbjct 120 PLLEHAIVCETPEGKEKGISLSEGMKSFAEVLADGSPDFTGPELGDDDVAVILYTSGTTG 179 Query 170 HPKGVVHTHESVHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSA-MRGVTLISM 228 PKG + TH++++S A A + + DR++ LPMFHV LT + + M G T++ M Sbjct 180 KPKGAMLTHQNIYSNAQDTADYLGINENDRVIAALPMFHVFCLTVALNAPLMNGGTVLIM 239 Query 229 PQFDATKVWSLIVEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIK 288 P+F K++ L EE+ I VP + NF+ Q + D R I+GGA MP AL++ Sbjct 240 PKFSPAKLFQLAREEKATIFAGVPTMYNFLYQYEGGSADDLRTLRLCISGGASMPVALLE 299 Query 289 IYAAK-NIEVVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHG 347 + K N+ + +GY L+E+ T D RK GS G T +V ++ V+ E+G Sbjct 300 NFEKKFNVIISEGYGLSEASPV-TCFNPLDRPRKPGSIG--TSIKNV----ENKVVNEYG 352 Query 348 E-------GEVVIKSDILLKEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKD 400 E GE+V++ ++K Y+ PE T A +GW TGD+ +D++GY YI DR K+ Sbjct 353 EEVPIGEVGELVVRGPNVMKGYYKMPEETSAALRDGWLHTGDLARMDEDGYFYIVDRKKE 412 Query 401 MIISGGENVYPAEIESVIIGVPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSEQQIVEY 460 MII GG NVYP E+E V+ P V E AVIG+PD +GE A VVA E++E +++ Y Sbjct 413 MIIVGGYNVYPREVEEVLYSHPDVVEAAVIGVPDPDYGEAVRAYVVAKNQELTEAELIAY 472 Query 461 CGTRLARYKLPKKVIFAEAIPRNPTGKILKTVLREQYSAT 500 C RLA+YK+P + F + +P+N TGKIL+ L+E+ +++ Sbjct 473 CRERLAKYKVPSAIDFLDELPKNATGKILRRALKERLASS 512 >gi|254391251|ref|ZP_05006456.1| feruloyl-CoA synthetase [Streptomyces clavuligerus ATCC 27064] gi|326444618|ref|ZP_08219352.1| substrate-CoA ligase [Streptomyces clavuligerus ATCC 27064] gi|197704943|gb|EDY50755.1| feruloyl-CoA synthetase [Streptomyces clavuligerus ATCC 27064] Length=521 Score = 322 bits (824), Expect = 1e-85, Method: Compositional matrix adjust. Identities = 197/506 (39%), Positives = 277/506 (55%), Gaps = 16/506 (3%) Query 2 KNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLM 61 + IG +R+RA +P A V D+R TYAQ + R A L LG+ +GDRV L Sbjct 4 QGIGSWIRRRARKTPHRCAVVH--RDLRYTYAQWDERTTRLAHHLRGLGVRRGDRVGFLG 61 Query 62 PNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRAQ 121 N + A +LGAV VP+NTRLAAPE+ L+DSG+ ++++ AP DA A+ Sbjct 62 ANHPALLETLFAAGQLGAVLVPLNTRLAAPELRHCLTDSGTSLLVH----APAFDAF-AR 116 Query 122 ADPPGTVT--DWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHE 179 P G D D+LA R DE DDN IMYTSGTTG KGVV +H Sbjct 117 DCPVGRTVPVDDTYEDALA-RADGTPLDESVSR---DDNAVIMYTSGTTGRAKGVVLSHG 172 Query 180 SVHSAASSWASTIDVRYRDRLLLPLPMFHVAALT-TVIFSAMRGVTLISMPQFDATKVWS 238 ++ + + D+ + LL P+FH AAL T + ++G TL+ F T+ Sbjct 173 NITWNSVNVLVDADLASDEVTLLSAPLFHTAALNMTCLPVLLKGGTLVLEESFSPTRTLD 232 Query 239 LIVEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVV 298 LI E V + VPA+ + F D R + GGAP+P ALI++Y + + + Sbjct 233 LIEEHGVTLMFGVPAMFQQIAAAERFDAADLSSVRTMLCGGAPVPPALIRLYKDRGLRFL 292 Query 299 QGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGE-GEVVIKSDI 357 QGY LTE+ G LL +E+AL KAGSAG F D+ + G E GE GE++++ Sbjct 293 QGYGLTEASPGVCLLDAENALTKAGSAGLPHFFNDIRIVDRAGDDVERGERGELLVEGPT 352 Query 358 LLKEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESV 417 + YW E AF +GW +GD+ D++GY+ + DR KDMIISGGEN+ P E+E Sbjct 353 VTSGYWGPSEENEAAFADGWLHSGDVVTQDEDGYVTVVDRAKDMIISGGENISPVEVEKA 412 Query 418 IIGVPGVSEVAVIGLPDEKWGEIAAAIVVADQNE-VSEQQIVEYCGTRLARYKLPKKVIF 476 + P V++ AVIG+PD WGE+ A++V + ++I+ + RLARYK+P V+ Sbjct 413 LFEHPAVADCAVIGVPDATWGEVGCAVIVRTPGAGATGEEILAFLDGRLARYKIPHAVVL 472 Query 477 AEAIPRNPTGKILKTVLREQYSATVP 502 +A+PRN TGK+ K LRE + T P Sbjct 473 TDALPRNSTGKVAKHRLREICATTAP 498 >gi|114799539|ref|YP_762100.1| feruloyl-CoA synthetase [Hyphomonas neptunium ATCC 15444] gi|114739713|gb|ABI77838.1| feruloyl-CoA synthetase [Hyphomonas neptunium ATCC 15444] Length=522 Score = 321 bits (823), Expect = 2e-85, Method: Compositional matrix adjust. Identities = 195/486 (41%), Positives = 276/486 (57%), Gaps = 26/486 (5%) Query 31 TYAQMNALANRCADVLTALGIAKGDRVALLMPNSVEFCCLFYGAAKLGAVAVPINTRLAA 90 ++A+++ +N+ A+ L LG+ GDRV L N+ + L +G AK V +NTRLA Sbjct 35 SFAELDRRSNQIANGLIRLGLQPGDRVGYLAKNTDAYYELLFGCAKSRTVMTGVNTRLAP 94 Query 91 PEVSFILSDSGSKVVIYGAPSAPVIDAIRAQADP-------PGTVTDWIGADSLAERLRS 143 PEV FILSD+ ++V+ G +ID I+A+ G DW D S Sbjct 95 PEVKFILSDAKARVLFVGKEFYAMIDQIKAELPDLVRIITLDGDREDW---DYFPSWRDS 151 Query 144 AAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHESVHSAASSWASTIDVRYRDR---L 200 A+ P ++ G+D++ +YTSGTTG PKGV T+++ + A + A ++ D + Sbjct 152 KPAEPPGLDVQGEDDVIQLYTSGTTGLPKGVQLTNDN-YRAFFTQAGMLEWSSYDAGEAI 210 Query 201 LLPLPMFHVAALTTVIFSAMRGVTLISMPQFDATKVWSLIVEERVCIGGAVPAILNFMRQ 260 + +P FHVA + + ++++G + + + D + LI E R+ VPA++ + Q Sbjct 211 MNAMPQFHVAGVNVGVLASLQGAKTVILREIDPQAILRLIPEHRIAHAFWVPAVILMLTQ 270 Query 261 VPEFAELDAPDFRYFITGGAPMPEALIK----IYAAKNIEVVQGYALTESCGGGTLLLSE 316 P E D + G +P+ EAL++ I A+ Q Y LTE+ G GT L E Sbjct 271 QPNIRETDFSSLKQVFYGASPISEALLRTAVEIMGAR---FTQLYGLTETVGAGTFLPPE 327 Query 317 ---DALRKAGSAGRATMFTDVAVRGDDGVIREHGE-GEVVIKSDILLKEYWNRPEATRDA 372 + K S G V V DG GE GE+VIKS ++K YWNRPEAT+DA Sbjct 328 AHDPSWGKLRSCGVPWPGAVVRVVDGDGKPVPTGEVGEIVIKSGFVMKGYWNRPEATQDA 387 Query 373 FDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVIIGVPGVSEVAVIGL 432 NG+F TGD G D++G+LYI DR+KDMI+SGGENVYPAE+E+ I G PGV++VAVIG+ Sbjct 388 VRNGFFHTGDAGYFDEDGFLYIHDRVKDMIVSGGENVYPAEVENAIFGAPGVADVAVIGV 447 Query 433 PDEKWGEIAAAIVVADQNE-VSEQQIVEYCGTRLARYKLPKKVIFAEAIPRNPTGKILKT 491 PDEKWGE AIVV E S + I+ + +A YK PK V F EA+PRNP+GKIL+ Sbjct 448 PDEKWGEAVKAIVVKKPGEDPSPESIIAWAKDHIAAYKAPKSVDFIEALPRNPSGKILRK 507 Query 492 VLREQY 497 LRE Y Sbjct 508 DLREPY 513 >gi|329937490|ref|ZP_08287048.1| O-succinylbenzoate-CoA ligase [Streptomyces griseoaurantiacus M045] gi|329303366|gb|EGG47253.1| O-succinylbenzoate-CoA ligase [Streptomyces griseoaurantiacus M045] Length=512 Score = 321 bits (823), Expect = 2e-85, Method: Compositional matrix adjust. Identities = 200/506 (40%), Positives = 278/506 (55%), Gaps = 11/506 (2%) Query 2 KNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLM 61 + IG +RA +PR A V D +TY ++ R A L ALG+A+GDRVA L Sbjct 4 QGIGSWPARRARKTPRRTAIVH--EDTHLTYRDLHERVLRLAHALRALGVARGDRVAYLG 61 Query 62 PNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRAQ 121 PN F + LGAV VP+NTRLAAPE+++ LSDSGS V+++ A A A A+ Sbjct 62 PNHPSFLETLFATGVLGAVFVPLNTRLAAPELAYNLSDSGSTVLVHAAGHAGTAAAAGAE 121 Query 122 ADPPGTVT---DWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTH 178 A +T GA E L A AD G D+ IMYTSGTTG PKG V +H Sbjct 122 APALRRITLGPAAEGALGYEELLAGAPADPLDEPVGPDEPCMIMYTSGTTGRPKGAVLSH 181 Query 179 ESVHSAASSWASTIDVRYRDRLLLPLPMFHVAALT-TVIFSAMRGVTLISMPQFDATKVW 237 ++ + + D+ + L+ P+FH A L T + + ++G ++ FDA V Sbjct 182 ANITWNSVNVLVDTDLAGDEVTLVVAPLFHTAGLNMTCLPTLLKGGRVVLHGAFDAGHVL 241 Query 238 SLIVEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEV 297 + RV VP + + M P + + D R GGAP+P I+ Y A+ + Sbjct 242 EAVAAHRVTYMFGVPTMYDAMAAHPRWRDADLSSLRSVSCGGAPVPARTIETYLARGLAF 301 Query 298 VQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGE-GEVVIKSD 356 QGY +TE+ G L E+A KAGSAG FTD V G DG GE GE++++ Sbjct 302 SQGYGMTEASPGVLFLDREEATAKAGSAGVPHFFTDTRVTGPDGGPAAPGEKGEILVRGP 361 Query 357 ILLKEYWNRPEATRDAF-DNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIE 415 ++ YW RP T AF ++GW RTGD+ D++GY YI DR+KDM +SGGENVYPAE+E Sbjct 362 NVMSGYWGRPADTAAAFTEDGWLRTGDVARTDEDGYAYIVDRVKDMFVSGGENVYPAEVE 421 Query 416 SVIIGVPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSEQQIVEYCGT---RLARYKLPK 472 ++G P V E AVIG+PD WGE+ A+VV + + E G RLA+YK+P+ Sbjct 422 DALLGHPAVRECAVIGVPDAVWGEVGHAVVVLEPGADAGADGAEILGRLHGRLAKYKIPR 481 Query 473 KVIFAEAIPRNPTGKILKTVLREQYS 498 V F +A+PR +GKI+K+ +R ++ Sbjct 482 TVAFVDALPRTASGKIVKSAVRRAHA 507 >gi|294816287|ref|ZP_06774930.1| Feruloyl-CoA synthetase [Streptomyces clavuligerus ATCC 27064] gi|294328886|gb|EFG10529.1| Feruloyl-CoA synthetase [Streptomyces clavuligerus ATCC 27064] Length=527 Score = 321 bits (823), Expect = 2e-85, Method: Compositional matrix adjust. Identities = 197/506 (39%), Positives = 277/506 (55%), Gaps = 16/506 (3%) Query 2 KNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLM 61 + IG +R+RA +P A V D+R TYAQ + R A L LG+ +GDRV L Sbjct 10 QGIGSWIRRRARKTPHRCAVVH--RDLRYTYAQWDERTTRLAHHLRGLGVRRGDRVGFLG 67 Query 62 PNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRAQ 121 N + A +LGAV VP+NTRLAAPE+ L+DSG+ ++++ AP DA A+ Sbjct 68 ANHPALLETLFAAGQLGAVLVPLNTRLAAPELRHCLTDSGTSLLVH----APAFDAF-AR 122 Query 122 ADPPGTVT--DWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHE 179 P G D D+LA R DE DDN IMYTSGTTG KGVV +H Sbjct 123 DCPVGRTVPVDDTYEDALA-RADGTPLDESVSR---DDNAVIMYTSGTTGRAKGVVLSHG 178 Query 180 SVHSAASSWASTIDVRYRDRLLLPLPMFHVAALT-TVIFSAMRGVTLISMPQFDATKVWS 238 ++ + + D+ + LL P+FH AAL T + ++G TL+ F T+ Sbjct 179 NITWNSVNVLVDADLASDEVTLLSAPLFHTAALNMTCLPVLLKGGTLVLEESFSPTRTLD 238 Query 239 LIVEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVV 298 LI E V + VPA+ + F D R + GGAP+P ALI++Y + + + Sbjct 239 LIEEHGVTLMFGVPAMFQQIAAAERFDAADLSSVRTMLCGGAPVPPALIRLYKDRGLRFL 298 Query 299 QGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGE-GEVVIKSDI 357 QGY LTE+ G LL +E+AL KAGSAG F D+ + G E GE GE++++ Sbjct 299 QGYGLTEASPGVCLLDAENALTKAGSAGLPHFFNDIRIVDRAGDDVERGERGELLVEGPT 358 Query 358 LLKEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESV 417 + YW E AF +GW +GD+ D++GY+ + DR KDMIISGGEN+ P E+E Sbjct 359 VTSGYWGPSEENEAAFADGWLHSGDVVTQDEDGYVTVVDRAKDMIISGGENISPVEVEKA 418 Query 418 IIGVPGVSEVAVIGLPDEKWGEIAAAIVVADQNE-VSEQQIVEYCGTRLARYKLPKKVIF 476 + P V++ AVIG+PD WGE+ A++V + ++I+ + RLARYK+P V+ Sbjct 419 LFEHPAVADCAVIGVPDATWGEVGCAVIVRTPGAGATGEEILAFLDGRLARYKIPHAVVL 478 Query 477 AEAIPRNPTGKILKTVLREQYSATVP 502 +A+PRN TGK+ K LRE + T P Sbjct 479 TDALPRNSTGKVAKHRLREICATTAP 504 >gi|296269589|ref|YP_003652221.1| AMP-dependent synthetase and ligase [Thermobispora bispora DSM 43833] gi|296092376|gb|ADG88328.1| AMP-dependent synthetase and ligase [Thermobispora bispora DSM 43833] Length=509 Score = 320 bits (821), Expect = 2e-85, Method: Compositional matrix adjust. Identities = 203/510 (40%), Positives = 289/510 (57%), Gaps = 25/510 (4%) Query 1 MKNIG---WMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRV 57 M+N G W R RA +SP A+V D +TYA+ + R A L A G+A GDRV Sbjct 1 MRNAGLGSWPAR-RARMSPGRTAFV--YEDRAVTYAEFHERVMRLASRLRAAGVAPGDRV 57 Query 58 ALLMPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDA 117 A L N V F + LGA+ VP+N RLAAPE+ ++L +SG+ ++IY A + A Sbjct 58 AYLGKNHVAFAESMFATHALGAIFVPLNFRLAAPEIDYMLENSGAVLLIYAPECAQTVRA 117 Query 118 I--------RAQADPPGTVTDWIGADSLAERLRSAAADEPA-VECGGDDNLFIMYTSGTT 168 + R PG ++ E S EP V DD I+YTSGTT Sbjct 118 LTGRHALRERVALGEPGP------GEAQYEAWLSEGVPEPIDVPVALDDTALILYTSGTT 171 Query 169 GHPKGVVHTHESVHSAASSWASTIDVRYRDRLLLPLPMFHVAALT-TVIFSAMRGVTLIS 227 G PKG + +H ++ + + +DV + L+ P+FHVAAL T++ + ++G + Sbjct 172 GRPKGAMLSHANLVWNCFNLLANVDVASDEVTLISAPLFHVAALNQTLLPTFLKGGCSVI 231 Query 228 MPQFDATKVWSLIVEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALI 287 MP +D + + LI + RV V + + P +A+ D R ++GGAP+P +LI Sbjct 232 MPSWDVDRCYDLIEKYRVTWMFGVTTMFAALASSPRWAQADLSSVRTLMSGGAPIPVSLI 291 Query 288 KIYAAKNIEVVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHG 347 + Y + + QGY LTE+ G T L + +++RKAGSAG FT+V V D E G Sbjct 292 RTYQERGLVFCQGYGLTETSPGATFLEAGESVRKAGSAGVPVFFTNVRVVRPDLTDVEPG 351 Query 348 E-GEVVIKSDILLKEYWNRPEATRDAF-DNGWFRTGDIGEIDDEGYLYIKDRLKDMIISG 405 E GEV+I+ + K YW PEAT AF + GWF +GD+ +D+EGY+YI DRLKDM ISG Sbjct 352 EPGEVIIQGPNVTKGYWGNPEATAAAFSEGGWFHSGDVATVDEEGYIYIVDRLKDMYISG 411 Query 406 GENVYPAEIESVIIGVPGVSEVAVIGLPDEKWGEIAAAIVVADQN-EVSEQQIVEYCGTR 464 GENVYPAE+E VI P V++VAV+G+PDEKWGE+ A +V V+ +++ E+ R Sbjct 412 GENVYPAEVEGVIFEHPAVAQVAVVGVPDEKWGEVGCAFIVTRPGMTVTAEELREFLRPR 471 Query 465 LARYKLPKKVIFAEAIPRNPTGKILKTVLR 494 LA+YK+P F + +P+ +GKI KT LR Sbjct 472 LAKYKIPVYFEFVDDLPKTGSGKIQKTALR 501 >gi|56419203|ref|YP_146521.1| long-chain-fatty-acid--CoA ligase [Geobacillus kaustophilus HTA426] gi|56379045|dbj|BAD74953.1| long-chain fatty-acid-CoA ligase [Geobacillus kaustophilus HTA426] Length=514 Score = 320 bits (821), Expect = 3e-85, Method: Compositional matrix adjust. Identities = 196/520 (38%), Positives = 284/520 (55%), Gaps = 31/520 (5%) Query 3 NIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLMP 62 N+ L Q A P +AYV R TY Q++ +R AD L LG+ +GD +ALL+ Sbjct 2 NVTTRLAQIAKQLPDKEAYV--FMKERCTYQQLDEAISRFADGLARLGVRRGDHIALLLG 59 Query 63 NSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVI----------YGAPSA 112 NS +F YGA +LGA +PIN E+S+IL + K VI Sbjct 60 NSPQFVIGLYGALRLGATVIPINPIYTPEEISYILHNGDVKAVIGLDLLAPLFVEAKKRL 119 Query 113 PVIDAIRAQADPPGT---VTDWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTG 169 P+++ P G V+ G S AE L + D E G DD I+YTSGTTG Sbjct 120 PLLEHAIVCETPEGKEKGVSLSEGMKSFAEVLADGSQDFTGPELGDDDVAVILYTSGTTG 179 Query 170 HPKGVVHTHESVHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSA-MRGVTLISM 228 PKG + TH++++S A A + + DR++ LPMFHV LT + + M G T++ M Sbjct 180 KPKGAMLTHKNIYSNAQDTADYLGINENDRVIAALPMFHVFCLTVALNAPLMNGGTVLIM 239 Query 229 PQFDATKVWSLIVEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIK 288 P+F K++ L EE+ I VP + NF+ Q + D R I+GGA MP AL++ Sbjct 240 PKFSPAKLFQLAREEKATIFAGVPTMYNFLYQYEGGSADDLRTLRLCISGGASMPVALLE 299 Query 289 IYAAK-NIEVVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHG 347 + K N+ + +GY L+E+ T D RK GS G T +V ++ V+ E+G Sbjct 300 NFEKKFNVIISEGYGLSEASPV-TCFNPLDRPRKPGSIG--TNIKNV----ENKVVNEYG 352 Query 348 E-------GEVVIKSDILLKEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKD 400 E GE+V++ ++K Y+ PE T A +GW TGD+ +D++GY YI DR K+ Sbjct 353 EEVPVGEVGELVVRGPNVMKGYYKMPEETSAALRDGWLHTGDLARMDEDGYFYIVDRKKE 412 Query 401 MIISGGENVYPAEIESVIIGVPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSEQQIVEY 460 MII GG NVYP E+E V+ P V E AVIG+PD +GE A VVA E++E +++ Y Sbjct 413 MIIVGGYNVYPREVEEVLYSHPDVVEAAVIGVPDPDYGEAVRAYVVAKNPELTEAELIAY 472 Query 461 CGTRLARYKLPKKVIFAEAIPRNPTGKILKTVLREQYSAT 500 C RLA+YK+P + F + +P+N TGKIL+ L+E+ +++ Sbjct 473 CRERLAKYKVPSAIDFLDELPKNATGKILRRALKERLASS 512 >gi|302546432|ref|ZP_07298774.1| O-succinylbenzoate-CoA ligase [Streptomyces hygroscopicus ATCC 53653] gi|302464050|gb|EFL27143.1| O-succinylbenzoate-CoA ligase [Streptomyces himastatinicus ATCC 53653] Length=492 Score = 320 bits (821), Expect = 3e-85, Method: Compositional matrix adjust. Identities = 197/498 (40%), Positives = 286/498 (58%), Gaps = 20/498 (4%) Query 3 NIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLMP 62 IG R+RA +P A V D TY +++A R A L A GI +GDRVA L P Sbjct 9 GIGSWPRRRARKTPGRIAIVH--GDDTQTYRELDATVTRFARALRARGIGRGDRVAYLGP 66 Query 63 NSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRAQA 122 N F + +GAV VP+NTRLAAPE++++LSDSG++++++ P A+A Sbjct 67 NHPSFLHALFATCAVGAVFVPLNTRLAAPELAYMLSDSGARLLLHD----PHCAETAAEA 122 Query 123 DPPGTV-TDWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHESV 181 P V TD + +R A AD DD IMYTSGTTG PKG V +H ++ Sbjct 123 GPGIAVATDHLVDGDGDDRADEAMAD--------DDPCMIMYTSGTTGRPKGAVLSHANI 174 Query 182 HSAASSWASTIDVRYRDRLLLPLPMFHVAALT-TVIFSAMRGVTLISMPQFDATKVWSLI 240 + + D+ + L+ P+FH AAL T + + ++G ++ P FDA ++ LI Sbjct 175 TWNSLNVLVDTDLASDEITLVTAPLFHTAALNMTCLPTLLKGGRVVIEPGFDAERMLDLI 234 Query 241 VEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVVQG 300 + V VP + + + P +A D R + GG+P+P ALI +Y + + +QG Sbjct 235 EGQGVTCLFGVPTMFAAVAESPRWATADLSSVRILMCGGSPVPRALIDVYLRRGLSFIQG 294 Query 301 YALTESCGGGTLLLSEDALRKAGSAGRATMFTDVA-VRGDDGVIREHGE-GEVVIKSDIL 358 Y +TE+ G LL E + KAGSAG FTDV VR D GE GE+++ + Sbjct 295 YGMTEASPGTLLLDREMSAVKAGSAGVPHFFTDVRLVRSDLTGEAAPGEPGEILVHGPHV 354 Query 359 LKEYWNRPEATRDAF-DNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESV 417 ++ Y+ P+AT +A + GWFR+GD+G D++GY++I DR+KDMIISGGENVYPAE+E+ Sbjct 355 MRGYYGLPDATAEALLEGGWFRSGDVGVRDEDGYVFIVDRVKDMIISGGENVYPAEVENA 414 Query 418 IIGVPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSE-QQIVEYCGTRLARYKLPKKVIF 476 + P V E AVIG+PD +WGE+ A+VV ++ Q ++++ RLA+YK+PK V F Sbjct 415 LYDHPDVVECAVIGMPDARWGEVGKAVVVLRAGAATDPQDLLDHLAGRLAKYKVPKAVEF 474 Query 477 AEAIPRNPTGKILKTVLR 494 A+PR +GKI K+ LR Sbjct 475 RTALPRTASGKIRKSALR 492 >gi|291334294|gb|ADD93956.1| predicted acid CoA ligase fadD13 [uncultured marine bacterium MedDCM-OCT-S09-C199] Length=514 Score = 320 bits (821), Expect = 3e-85, Method: Compositional matrix adjust. Identities = 184/488 (38%), Positives = 275/488 (57%), Gaps = 13/488 (2%) Query 20 AYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLMPNSVEFCCLFYGAAKLGA 79 A V+ T +TY + A+R A+ L + G+ KGDRV LL PN E + +K+GA Sbjct 21 AIVDLDTQEEVTYGALEDRASRLAEWLQSQGVIKGDRVTLLTPNCPEVFEAQFACSKIGA 80 Query 80 VAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRAQADPPGTVT--DWIGADSL 137 + +P+N RL PE+ +IL DS KV+IY A A + + + P ++ A + Sbjct 81 ICIPLNWRLTVPELEYILGDSAPKVLIYDAKFADAAEQLISLCSVPNSLMLDAHDAACAY 140 Query 138 AERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHESVHSAASSWASTIDVRYR 197 + L + VEC DD IMYTSGTTGHPKG + T A + + Sbjct 141 KQTLAALTGAYQGVECTLDDVAMIMYTSGTTGHPKGAMITFGMNFYNAVNLGIPAGISSN 200 Query 198 DRLLLPLPMFHVAALTTVIFSAMR-GVTLISMPQFDATKVWSLIVEERVCIGG--AVPAI 254 L+ LP+FH + + G ++ M FD + +++ + + + VPA Sbjct 201 SIQLVVLPLFHTGGMNCYANPILHAGGRILLMRDFDPGRALAVLGDASLGVTHFFGVPAP 260 Query 255 LNFMRQVPEFAELDAPDFRYFIT---GGAPMPEALIKIYAAKNIEVVQGYALTESCGGGT 311 FM Q P+F DA D +T GGAP EA+++ + + + ++QG+ +TE+ GGT Sbjct 261 YQFMMQHPDF---DATDLSRIVTAGVGGAPCAEAILRGWIDRGVPLIQGWGMTETSPGGT 317 Query 312 LLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGE-GEVVIKSDILLKEYWNRPEATR 370 L + D +RK GSAG+A + T++ + DDG GE GE++I+ + YWN+ AT Sbjct 318 ALDAADVMRKIGSAGKALLHTELKIIDDDGATLPWGEVGELLIRGPNITPGYWNKAGATA 377 Query 371 DAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVIIGVPGVSEVAVI 430 D+F +GW +TGD DDEG++YI DR KDM ISGGENVYPAE+E+V+ + V+E A+I Sbjct 378 DSFVDGWLKTGDAARFDDEGFIYIVDRWKDMYISGGENVYPAEVENVLYQLEQVAEAAII 437 Query 431 GLPDEKWGEIAAAIVVADQNE-VSEQQIVEYCGTRLARYKLPKKVIFAEAIPRNPTGKIL 489 G+PDE+WGE A++V + + ++ +C LA++K+P V F EA+PRN TGK+L Sbjct 438 GVPDERWGETGKAVLVLKPGQALDADAVIGHCLANLAKFKVPSSVEFIEALPRNATGKVL 497 Query 490 KTVLREQY 497 K LR+QY Sbjct 498 KRTLRDQY 505 Lambda K H 0.319 0.136 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Effective search space used: 1086623926964 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects Posted date: Sep 5, 2011 4:36 AM Number of letters in database: 5,219,829,388 Number of sequences in database: 15,229,318 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 11 Window for multiple hits: 40