BLASTP 2.2.25+
Reference:
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database
search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
15,229,318 sequences; 5,219,829,388 total letters
Query= Rv3224A
Length=62
Score E
Sequences producing significant alignments: (Bits) Value
gi|31794404|ref|NP_856897.1| hypothetical protein Mb3252 [Mycoba... 121 3e-26
gi|148824425|ref|YP_001289179.1| hypothetical protein TBFG_13252... 119 2e-25
gi|340628204|ref|YP_004746656.1| hypothetical protein MCAN_32431... 41.2 0.054
gi|254819141|ref|ZP_05224142.1| hypothetical protein MintA_04404... 39.7 0.15
gi|5305776|gb|AAD41809.1|AF144091_3 unknown [Mycobacterium smegm... 36.6 1.4
gi|118470403|ref|YP_886279.1| YbaK/ebsC protein [Mycobacterium s... 36.6 1.4
gi|326381836|ref|ZP_08203529.1| hypothetical protein SCNU_02782 ... 35.8 2.2
gi|183981355|ref|YP_001849646.1| hypothetical protein MMAR_1332 ... 35.8 2.4
gi|15827348|ref|NP_301611.1| hypothetical protein ML0799 [Mycoba... 35.8 2.5
gi|118618029|ref|YP_906361.1| hypothetical protein MUL_2547 [Myc... 35.0 3.7
gi|283458263|ref|YP_003362882.1| hypothetical protein RMDY18_123... 34.7 4.9
gi|120402776|ref|YP_952605.1| ybaK/ebsC protein [Mycobacterium v... 33.5 9.8
>gi|31794404|ref|NP_856897.1| hypothetical protein Mb3252 [Mycobacterium bovis AF2122/97]
gi|57117076|ref|YP_177946.1| hypothetical protein Rv3224A [Mycobacterium tuberculosis H37Rv]
gi|121639113|ref|YP_979337.1| hypothetical protein BCG_3253 [Mycobacterium bovis BCG str. Pasteur
1173P2]
45 more sequence titles
Length=62
Score = 121 bits (304), Expect = 3e-26, Method: Compositional matrix adjust.
Identities = 61/62 (99%), Positives = 62/62 (100%), Gaps = 0/62 (0%)
Query 1 VRRSASTCGWKTPTRRGTSRPSDSKTLILELPDERAVAIVPVPSKLSLKAAGGPRGAQSG 60
+RRSASTCGWKTPTRRGTSRPSDSKTLILELPDERAVAIVPVPSKLSLKAAGGPRGAQSG
Sbjct 1 MRRSASTCGWKTPTRRGTSRPSDSKTLILELPDERAVAIVPVPSKLSLKAAGGPRGAQSG 60
Query 61 HG 62
HG
Sbjct 61 HG 62
>gi|148824425|ref|YP_001289179.1| hypothetical protein TBFG_13252 [Mycobacterium tuberculosis F11]
gi|148722952|gb|ABR07577.1| conserved hypothetical protein [Mycobacterium tuberculosis F11]
Length=62
Score = 119 bits (298), Expect = 2e-25, Method: Compositional matrix adjust.
Identities = 60/62 (97%), Positives = 61/62 (99%), Gaps = 0/62 (0%)
Query 1 VRRSASTCGWKTPTRRGTSRPSDSKTLILELPDERAVAIVPVPSKLSLKAAGGPRGAQSG 60
+RRSASTCGWKTPTRRGTSRPSDSKTL LELPDERAVAIVPVPSKLSLKAAGGPRGAQSG
Sbjct 1 MRRSASTCGWKTPTRRGTSRPSDSKTLSLELPDERAVAIVPVPSKLSLKAAGGPRGAQSG 60
Query 61 HG 62
HG
Sbjct 61 HG 62
>gi|340628204|ref|YP_004746656.1| hypothetical protein MCAN_32431 [Mycobacterium canettii CIPT
140010059]
gi|340006394|emb|CCC45574.1| putative uncharacterized protein [Mycobacterium canettii CIPT
140010059]
Length=70
Score = 41.2 bits (95), Expect = 0.054, Method: Compositional matrix adjust.
Identities = 19/22 (87%), Positives = 21/22 (96%), Gaps = 0/22 (0%)
Query 1 VRRSASTCGWKTPTRRGTSRPS 22
++RSASTCGWKTPTR GTSRPS
Sbjct 1 MQRSASTCGWKTPTRLGTSRPS 22
>gi|254819141|ref|ZP_05224142.1| hypothetical protein MintA_04404 [Mycobacterium intracellulare
ATCC 13950]
Length=152
Score = 39.7 bits (91), Expect = 0.15, Method: Compositional matrix adjust.
Identities = 21/32 (66%), Positives = 23/32 (72%), Gaps = 0/32 (0%)
Query 25 KTLILELPDERAVAIVPVPSKLSLKAAGGPRG 56
KTLI+ LP E AVAIVPVP +LSLKA G
Sbjct 43 KTLIVALPGELAVAIVPVPWRLSLKAVAAALG 74
>gi|5305776|gb|AAD41809.1|AF144091_3 unknown [Mycobacterium smegmatis str. MC2 155]
Length=185
Score = 36.6 bits (83), Expect = 1.4, Method: Compositional matrix adjust.
Identities = 17/25 (68%), Positives = 21/25 (84%), Gaps = 0/25 (0%)
Query 25 KTLILELPDERAVAIVPVPSKLSLK 49
KTL++ LP AVA+VPVP+KLSLK
Sbjct 75 KTLVIALPKGLAVAVVPVPTKLSLK 99
>gi|118470403|ref|YP_886279.1| YbaK/ebsC protein [Mycobacterium smegmatis str. MC2 155]
gi|118171690|gb|ABK72586.1| YbaK/ebsC protein [Mycobacterium smegmatis str. MC2 155]
Length=163
Score = 36.6 bits (83), Expect = 1.4, Method: Compositional matrix adjust.
Identities = 17/25 (68%), Positives = 21/25 (84%), Gaps = 0/25 (0%)
Query 25 KTLILELPDERAVAIVPVPSKLSLK 49
KTL++ LP AVA+VPVP+KLSLK
Sbjct 53 KTLVIALPKGLAVAVVPVPTKLSLK 77
>gi|326381836|ref|ZP_08203529.1| hypothetical protein SCNU_02782 [Gordonia neofelifaecis NRRL
B-59395]
gi|326199262|gb|EGD56443.1| hypothetical protein SCNU_02782 [Gordonia neofelifaecis NRRL
B-59395]
Length=168
Score = 35.8 bits (81), Expect = 2.2, Method: Compositional matrix adjust.
Identities = 19/34 (56%), Positives = 26/34 (77%), Gaps = 1/34 (2%)
Query 25 KTLILELPDER-AVAIVPVPSKLSLKAAGGPRGA 57
KTL+++L D R VA++PVP+KLSLK+A GA
Sbjct 59 KTLVVDLGDGRLGVAVIPVPAKLSLKSAAKALGA 92
>gi|183981355|ref|YP_001849646.1| hypothetical protein MMAR_1332 [Mycobacterium marinum M]
gi|183174681|gb|ACC39791.1| conserved hypothetical membrane protein [Mycobacterium marinum
M]
Length=151
Score = 35.8 bits (81), Expect = 2.4, Method: Compositional matrix adjust.
Identities = 17/23 (74%), Positives = 20/23 (87%), Gaps = 0/23 (0%)
Query 25 KTLILELPDERAVAIVPVPSKLS 47
KTLI+ LP E AVA+VPVPS+LS
Sbjct 43 KTLIIALPGELAVAVVPVPSRLS 65
>gi|15827348|ref|NP_301611.1| hypothetical protein ML0799 [Mycobacterium leprae TN]
gi|221229826|ref|YP_002503242.1| hypothetical protein MLBr_00799 [Mycobacterium leprae Br4923]
gi|13092897|emb|CAC30308.1| conserved hypothetical protein [Mycobacterium leprae]
gi|219932933|emb|CAR70893.1| conserved hypothetical protein [Mycobacterium leprae Br4923]
Length=135
Score = 35.8 bits (81), Expect = 2.5, Method: Compositional matrix adjust.
Identities = 17/26 (66%), Positives = 21/26 (81%), Gaps = 0/26 (0%)
Query 24 SKTLILELPDERAVAIVPVPSKLSLK 49
SKTLI+ LP E A+AI+ PS+LSLK
Sbjct 22 SKTLIIALPRELAIAILSAPSRLSLK 47
>gi|118618029|ref|YP_906361.1| hypothetical protein MUL_2547 [Mycobacterium ulcerans Agy99]
gi|118570139|gb|ABL04890.1| conserved hypothetical membrane protein [Mycobacterium ulcerans
Agy99]
Length=111
Score = 35.0 bits (79), Expect = 3.7, Method: Compositional matrix adjust.
Identities = 16/23 (70%), Positives = 20/23 (87%), Gaps = 0/23 (0%)
Query 25 KTLILELPDERAVAIVPVPSKLS 47
KTL++ LP E AVA+VPVPS+LS
Sbjct 3 KTLVIALPGEFAVAVVPVPSRLS 25
>gi|283458263|ref|YP_003362882.1| hypothetical protein RMDY18_12300 [Rothia mucilaginosa DY-18]
gi|283134297|dbj|BAI65062.1| uncharacterized conserved protein [Rothia mucilaginosa DY-18]
Length=169
Score = 34.7 bits (78), Expect = 4.9, Method: Compositional matrix adjust.
Identities = 20/54 (38%), Positives = 30/54 (56%), Gaps = 0/54 (0%)
Query 6 STCGWKTPTRRGTSRPSDSKTLILELPDERAVAIVPVPSKLSLKAAGGPRGAQS 59
++ G + + G S KTL++ + AVAIVPV KL++KAA G +S
Sbjct 42 TSFGEEAAKKLGASEEQVFKTLLIVHEKDFAVAIVPVSGKLNIKAAAAALGWKS 95
>gi|120402776|ref|YP_952605.1| ybaK/ebsC protein [Mycobacterium vanbaalenii PYR-1]
gi|119955594|gb|ABM12599.1| ybaK/ebsC protein [Mycobacterium vanbaalenii PYR-1]
Length=178
Score = 33.5 bits (75), Expect = 9.8, Method: Compositional matrix adjust.
Identities = 16/25 (64%), Positives = 20/25 (80%), Gaps = 0/25 (0%)
Query 25 KTLILELPDERAVAIVPVPSKLSLK 49
KTL++ LP VA++PVPSKLSLK
Sbjct 69 KTLVVALPKGLGVAVLPVPSKLSLK 93
Lambda K H
0.312 0.129 0.384
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 131656912128
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Sep 5, 2011 4:36 AM
Number of letters in database: 5,219,829,388
Number of sequences in database: 15,229,318
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40