BLASTP 2.2.25+


Reference:
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database
search programs", Nucleic Acids Res. 25:3389-3402.



Reference for composition-based statistics:
Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.



Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
           15,229,318 sequences; 5,219,829,388 total letters



Query= Rv3373

Length=213
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

gi|31794556|ref|NP_857049.1|  enoyl-CoA hydratase [Mycobacterium ...   431    3e-119
gi|15610509|ref|NP_217890.1|  enoyl-CoA hydratase [Mycobacterium ...   429    9e-119
gi|289444971|ref|ZP_06434715.1|  LOW QUALITY PROTEIN: enoyl-CoA h...   429    1e-118
gi|298526854|ref|ZP_07014263.1|  conserved hypothetical protein [...   428    2e-118
gi|340628354|ref|YP_004746806.1|  putative enoyl-CoA hydratase EC...   427    5e-118
gi|297636034|ref|ZP_06953814.1|  enoyl-CoA hydratase [Mycobacteri...   419    1e-115
gi|167966833|ref|ZP_02549110.1|  putative enoyl-CoA hydratase ech...   413    9e-114
gi|289555652|ref|ZP_06444862.1|  enoyl-CoA hydratase echA18_1 [My...   372    2e-101
gi|308370243|ref|ZP_07420778.2|  LOW QUALITY PROTEIN: enoyl-CoA h...   326    1e-87 
gi|289763556|ref|ZP_06522934.1|  enoyl-CoA hydratase echA18 [Myco...   299    2e-79 
gi|240172692|ref|ZP_04751351.1|  putative enoyl-CoA hydratase [My...   275    4e-72 
gi|324999509|ref|ZP_08120621.1|  enoyl-CoA hydratase [Pseudonocar...   222    3e-56 
gi|221633735|ref|YP_002522961.1|  enoyl-CoA hydratase [Thermomicr...   157    7e-37 
gi|121534275|ref|ZP_01666099.1|  Enoyl-CoA hydratase/isomerase [T...   138    6e-31 
gi|344329229|gb|EGW40577.1|  enoyl-CoA hydratase/isomerase family...   137    1e-30 
gi|254479680|ref|ZP_05092977.1|  enoyl-CoA hydratase/isomerase fa...   137    1e-30 
gi|338814860|ref|ZP_08626835.1|  enoyl-CoA hydratase/isomerase [A...   135    6e-30 
gi|150015211|ref|YP_001307465.1|  3-hydroxybutyryl-CoA dehydratas...   134    6e-30 
gi|304317109|ref|YP_003852254.1|  enoyl-CoA hydratase/isomerase [...   133    1e-29 
gi|20807045|ref|NP_622216.1|  3-hydroxybutyryl-CoA dehydratase [T...   133    2e-29 
gi|119896971|ref|YP_932184.1|  putative carnitinyl-CoA dehydratas...   132    2e-29 
gi|168186033|ref|ZP_02620668.1|  3-hydroxybutyryl-CoA dehydratase...   132    3e-29 
gi|1903327|emb|CAB07495.1|  crotonase [Thermoanaerobacterium ther...   132    3e-29 
gi|121535320|ref|ZP_01667133.1|  Enoyl-CoA hydratase/isomerase [T...   132    3e-29 
gi|150016904|ref|YP_001309158.1|  3-hydroxybutyryl-CoA dehydratas...   132    4e-29 
gi|118444510|ref|YP_878945.1|  3-hydroxybutyryl-CoA dehydratase [...   131    6e-29 
gi|339323428|ref|YP_004682322.1|  CaiB/baiF CoA-transferase famil...   130    1e-28 
gi|254517612|ref|ZP_05129668.1|  3-hydroxybutyryl-CoA dehydratase...   130    1e-28 
gi|194289756|ref|YP_002005663.1|  enoyl-CoA hydratase/isomerase [...   130    1e-28 
gi|71909622|ref|YP_287209.1|  enoyl-CoA hydratase/isomerase [Dech...   130    1e-28 
gi|302875799|ref|YP_003844432.1|  Enoyl-CoA hydratase/isomerase [...   129    2e-28 
gi|187932888|ref|YP_001884608.1|  3-hydroxybutyryl-CoA dehydratas...   129    2e-28 
gi|188027000|emb|CAQ53134.1|  3-hydroxybutyryl-CoA dehydratase [C...   129    2e-28 
gi|338975489|ref|ZP_08630840.1|  enoyl-CoA hydratase [Bradyrhizob...   129    2e-28 
gi|110803399|ref|YP_699562.1|  3-hydroxybutyryl-CoA dehydratase [...   129    2e-28 
gi|144897474|emb|CAM74338.1|  Enoyl-CoA hydratase/carnithine race...   129    2e-28 
gi|225182057|ref|ZP_03735488.1|  Enoyl-CoA hydratase/isomerase [D...   129    3e-28 
gi|83310414|ref|YP_420678.1|  enoyl-CoA hydratase/carnithine race...   129    3e-28 
gi|169347081|ref|ZP_02866023.1|  3-hydroxybutyryl-CoA dehydratase...   129    3e-28 
gi|168216734|ref|ZP_02642359.1|  3-hydroxybutyryl-CoA dehydratase...   129    3e-28 
gi|18311283|ref|NP_563217.1|  3-hydroxybutyryl-CoA dehydratase [C...   129    3e-28 
gi|311107586|ref|YP_003980439.1|  enoyl-CoA hydratase [Achromobac...   129    3e-28 
gi|332284823|ref|YP_004416734.1|  enoyl-CoA hydratase [Pusillimon...   129    4e-28 
gi|56477517|ref|YP_159106.1|  enoyl-CoA hydratase/carnithine race...   129    4e-28 
gi|182419479|ref|ZP_02950731.1|  3-hydroxybutyryl-CoA dehydratase...   129    4e-28 
gi|134299635|ref|YP_001113131.1|  enoyl-CoA hydratase/isomerase [...   129    4e-28 
gi|302527841|ref|ZP_07280183.1|  cyclohexa-1,5-dienecarbonyl-CoA ...   129    4e-28 
gi|338195379|gb|EGO87669.1|  3-hydroxybutyryl-CoA dehydratase [Cl...   128    4e-28 
gi|333978267|ref|YP_004516212.1|  3-hydroxybutyryl-CoA dehydratas...   128    4e-28 
gi|227485263|ref|ZP_03915579.1|  3-hydroxybutyryl-CoA dehydratase...   128    4e-28 


>gi|31794556|ref|NP_857049.1| enoyl-CoA hydratase [Mycobacterium bovis AF2122/97]
 gi|121639300|ref|YP_979524.1| putative enoyl-CoA hydratase echA18 [Mycobacterium bovis BCG 
str. Pasteur 1173P2]
 gi|224991797|ref|YP_002646486.1| putative enoyl-CoA hydratase [Mycobacterium bovis BCG str. Tokyo 
172]
 12 more sequence titles
 Length=296

 Score =  431 bits (1108),  Expect = 3e-119, Method: Compositional matrix adjust.
 Identities = 213/213 (100%), Positives = 213/213 (100%), Gaps = 0/213 (0%)

Query  1    MRRRAMTKMDEASNPCGGDIEAEMCQLMREQPPAEGVVDRVALQRHRNVALITLSHPQAQ  60
            MRRRAMTKMDEASNPCGGDIEAEMCQLMREQPPAEGVVDRVALQRHRNVALITLSHPQAQ
Sbjct  1    MRRRAMTKMDEASNPCGGDIEAEMCQLMREQPPAEGVVDRVALQRHRNVALITLSHPQAQ  60

Query  61   NALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYN  120
            NALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYN
Sbjct  61   NALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYN  120

Query  121  ESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIATDDARFGIPLGKLGVTTGF  180
            ESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIATDDARFGIPLGKLGVTTGF
Sbjct  121  ESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIATDDARFGIPLGKLGVTTGF  180

Query  181  TEADTVARLIGPAALKYLLFSGELIGIEEAARW  213
            TEADTVARLIGPAALKYLLFSGELIGIEEAARW
Sbjct  181  TEADTVARLIGPAALKYLLFSGELIGIEEAARW  213


>gi|15610509|ref|NP_217890.1| enoyl-CoA hydratase [Mycobacterium tuberculosis H37Rv]
 gi|148663236|ref|YP_001284759.1| enoyl-CoA hydratase EchA18 [Mycobacterium tuberculosis H37Ra]
 gi|148824582|ref|YP_001289336.1| enoyl-CoA hydratase echA18 [Mycobacterium tuberculosis F11]
 27 more sequence titles
 Length=213

 Score =  429 bits (1104),  Expect = 9e-119, Method: Compositional matrix adjust.
 Identities = 213/213 (100%), Positives = 213/213 (100%), Gaps = 0/213 (0%)

Query  1    MRRRAMTKMDEASNPCGGDIEAEMCQLMREQPPAEGVVDRVALQRHRNVALITLSHPQAQ  60
            MRRRAMTKMDEASNPCGGDIEAEMCQLMREQPPAEGVVDRVALQRHRNVALITLSHPQAQ
Sbjct  1    MRRRAMTKMDEASNPCGGDIEAEMCQLMREQPPAEGVVDRVALQRHRNVALITLSHPQAQ  60

Query  61   NALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYN  120
            NALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYN
Sbjct  61   NALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYN  120

Query  121  ESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIATDDARFGIPLGKLGVTTGF  180
            ESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIATDDARFGIPLGKLGVTTGF
Sbjct  121  ESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIATDDARFGIPLGKLGVTTGF  180

Query  181  TEADTVARLIGPAALKYLLFSGELIGIEEAARW  213
            TEADTVARLIGPAALKYLLFSGELIGIEEAARW
Sbjct  181  TEADTVARLIGPAALKYLLFSGELIGIEEAARW  213


>gi|289444971|ref|ZP_06434715.1| LOW QUALITY PROTEIN: enoyl-CoA hydratase echA18 [Mycobacterium 
tuberculosis T46]
 gi|339633381|ref|YP_004725023.1| enoyl-CoA hydratase [Mycobacterium africanum GM041182]
 gi|289417890|gb|EFD15130.1| LOW QUALITY PROTEIN: enoyl-CoA hydratase echA18 [Mycobacterium 
tuberculosis T46]
 gi|339332737|emb|CCC28457.1| putative enoyl-CoA hydratase ECHA18 (enoyl hydrase) [Mycobacterium 
africanum GM041182]
Length=214

 Score =  429 bits (1104),  Expect = 1e-118, Method: Compositional matrix adjust.
 Identities = 213/213 (100%), Positives = 213/213 (100%), Gaps = 0/213 (0%)

Query  1    MRRRAMTKMDEASNPCGGDIEAEMCQLMREQPPAEGVVDRVALQRHRNVALITLSHPQAQ  60
            MRRRAMTKMDEASNPCGGDIEAEMCQLMREQPPAEGVVDRVALQRHRNVALITLSHPQAQ
Sbjct  1    MRRRAMTKMDEASNPCGGDIEAEMCQLMREQPPAEGVVDRVALQRHRNVALITLSHPQAQ  60

Query  61   NALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYN  120
            NALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYN
Sbjct  61   NALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYN  120

Query  121  ESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIATDDARFGIPLGKLGVTTGF  180
            ESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIATDDARFGIPLGKLGVTTGF
Sbjct  121  ESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIATDDARFGIPLGKLGVTTGF  180

Query  181  TEADTVARLIGPAALKYLLFSGELIGIEEAARW  213
            TEADTVARLIGPAALKYLLFSGELIGIEEAARW
Sbjct  181  TEADTVARLIGPAALKYLLFSGELIGIEEAARW  213


>gi|298526854|ref|ZP_07014263.1| conserved hypothetical protein [Mycobacterium tuberculosis 94_M4241A]
 gi|298496648|gb|EFI31942.1| conserved hypothetical protein [Mycobacterium tuberculosis 94_M4241A]
Length=213

 Score =  428 bits (1101),  Expect = 2e-118, Method: Compositional matrix adjust.
 Identities = 212/213 (99%), Positives = 213/213 (100%), Gaps = 0/213 (0%)

Query  1    MRRRAMTKMDEASNPCGGDIEAEMCQLMREQPPAEGVVDRVALQRHRNVALITLSHPQAQ  60
            MRRRAMTKMDEASNPCGGDIEAEMCQLMREQPPAEGVVDRVALQRHRNVALITLSHPQAQ
Sbjct  1    MRRRAMTKMDEASNPCGGDIEAEMCQLMREQPPAEGVVDRVALQRHRNVALITLSHPQAQ  60

Query  61   NALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYN  120
            NALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYN
Sbjct  61   NALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYN  120

Query  121  ESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIATDDARFGIPLGKLGVTTGF  180
            ESLAVCLRALTTMPIPVIAAV+GLAVGGGCELATACDVCIATDDARFGIPLGKLGVTTGF
Sbjct  121  ESLAVCLRALTTMPIPVIAAVQGLAVGGGCELATACDVCIATDDARFGIPLGKLGVTTGF  180

Query  181  TEADTVARLIGPAALKYLLFSGELIGIEEAARW  213
            TEADTVARLIGPAALKYLLFSGELIGIEEAARW
Sbjct  181  TEADTVARLIGPAALKYLLFSGELIGIEEAARW  213


>gi|340628354|ref|YP_004746806.1| putative enoyl-CoA hydratase ECHA18 [Mycobacterium canettii CIPT 
140010059]
 gi|340006544|emb|CCC45730.1| putative enoyl-CoA hydratase ECHA18 (enoyl hydrase) (unsaturated 
acyl-CoA hydratase) (crotonase) [Mycobacterium canettii 
CIPT 140010059]
Length=214

 Score =  427 bits (1098),  Expect = 5e-118, Method: Compositional matrix adjust.
 Identities = 212/213 (99%), Positives = 212/213 (99%), Gaps = 0/213 (0%)

Query  1    MRRRAMTKMDEASNPCGGDIEAEMCQLMREQPPAEGVVDRVALQRHRNVALITLSHPQAQ  60
            MRRRAMTKMDEASNPCGGDIEAEMCQLMREQPPAEGVVDRVALQRHRNVALITLSHPQAQ
Sbjct  1    MRRRAMTKMDEASNPCGGDIEAEMCQLMREQPPAEGVVDRVALQRHRNVALITLSHPQAQ  60

Query  61   NALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYN  120
            NALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYN
Sbjct  61   NALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYN  120

Query  121  ESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIATDDARFGIPLGKLGVTTGF  180
            ESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIA DDARFGIPLGKLGVTTGF
Sbjct  121  ESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIAADDARFGIPLGKLGVTTGF  180

Query  181  TEADTVARLIGPAALKYLLFSGELIGIEEAARW  213
            TEADTVARLIGPAALKYLLFSGELIGIEEAARW
Sbjct  181  TEADTVARLIGPAALKYLLFSGELIGIEEAARW  213


>gi|297636034|ref|ZP_06953814.1| enoyl-CoA hydratase [Mycobacterium tuberculosis KZN 4207]
 gi|339296199|gb|AEJ48310.1| putative enoyl-CoA hydratase echA18 [Mycobacterium tuberculosis 
CCDC5079]
 gi|339299802|gb|AEJ51912.1| putative enoyl-CoA hydratase echA18 [Mycobacterium tuberculosis 
CCDC5180]
Length=208

 Score =  419 bits (1078),  Expect = 1e-115, Method: Compositional matrix adjust.
 Identities = 208/208 (100%), Positives = 208/208 (100%), Gaps = 0/208 (0%)

Query  6    MTKMDEASNPCGGDIEAEMCQLMREQPPAEGVVDRVALQRHRNVALITLSHPQAQNALNL  65
            MTKMDEASNPCGGDIEAEMCQLMREQPPAEGVVDRVALQRHRNVALITLSHPQAQNALNL
Sbjct  1    MTKMDEASNPCGGDIEAEMCQLMREQPPAEGVVDRVALQRHRNVALITLSHPQAQNALNL  60

Query  66   ASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYNESLAV  125
            ASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYNESLAV
Sbjct  61   ASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYNESLAV  120

Query  126  CLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIATDDARFGIPLGKLGVTTGFTEADT  185
            CLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIATDDARFGIPLGKLGVTTGFTEADT
Sbjct  121  CLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIATDDARFGIPLGKLGVTTGFTEADT  180

Query  186  VARLIGPAALKYLLFSGELIGIEEAARW  213
            VARLIGPAALKYLLFSGELIGIEEAARW
Sbjct  181  VARLIGPAALKYLLFSGELIGIEEAARW  208


>gi|167966833|ref|ZP_02549110.1| putative enoyl-CoA hydratase echA18 [Mycobacterium tuberculosis 
H37Ra]
 gi|254552476|ref|ZP_05142923.1| putative enoyl-CoA hydratase [Mycobacterium tuberculosis '98-R604 
INH-RIF-EM']
 gi|294995857|ref|ZP_06801548.1| enoyl-CoA hydratase [Mycobacterium tuberculosis 210]
 gi|297733033|ref|ZP_06962151.1| enoyl-CoA hydratase [Mycobacterium tuberculosis KZN R506]
 gi|313660365|ref|ZP_07817245.1| enoyl-CoA hydratase [Mycobacterium tuberculosis KZN V2475]
Length=205

 Score =  413 bits (1061),  Expect = 9e-114, Method: Compositional matrix adjust.
 Identities = 205/205 (100%), Positives = 205/205 (100%), Gaps = 0/205 (0%)

Query  9    MDEASNPCGGDIEAEMCQLMREQPPAEGVVDRVALQRHRNVALITLSHPQAQNALNLASW  68
            MDEASNPCGGDIEAEMCQLMREQPPAEGVVDRVALQRHRNVALITLSHPQAQNALNLASW
Sbjct  1    MDEASNPCGGDIEAEMCQLMREQPPAEGVVDRVALQRHRNVALITLSHPQAQNALNLASW  60

Query  69   RRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYNESLAVCLR  128
            RRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYNESLAVCLR
Sbjct  61   RRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYNESLAVCLR  120

Query  129  ALTTMPIPVIAAVRGLAVGGGCELATACDVCIATDDARFGIPLGKLGVTTGFTEADTVAR  188
            ALTTMPIPVIAAVRGLAVGGGCELATACDVCIATDDARFGIPLGKLGVTTGFTEADTVAR
Sbjct  121  ALTTMPIPVIAAVRGLAVGGGCELATACDVCIATDDARFGIPLGKLGVTTGFTEADTVAR  180

Query  189  LIGPAALKYLLFSGELIGIEEAARW  213
            LIGPAALKYLLFSGELIGIEEAARW
Sbjct  181  LIGPAALKYLLFSGELIGIEEAARW  205


>gi|289555652|ref|ZP_06444862.1| enoyl-CoA hydratase echA18_1 [Mycobacterium tuberculosis KZN 
605]
 gi|289440284|gb|EFD22777.1| enoyl-CoA hydratase echA18_1 [Mycobacterium tuberculosis KZN 
605]
Length=186

 Score =  372 bits (955),  Expect = 2e-101, Method: Compositional matrix adjust.
 Identities = 186/186 (100%), Positives = 186/186 (100%), Gaps = 0/186 (0%)

Query  28   MREQPPAEGVVDRVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAV  87
            MREQPPAEGVVDRVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAV
Sbjct  1    MREQPPAEGVVDRVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAV  60

Query  88   VLRGAGDKAFAAGADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVG  147
            VLRGAGDKAFAAGADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVG
Sbjct  61   VLRGAGDKAFAAGADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVG  120

Query  148  GGCELATACDVCIATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGI  207
            GGCELATACDVCIATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGI
Sbjct  121  GGCELATACDVCIATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGI  180

Query  208  EEAARW  213
            EEAARW
Sbjct  181  EEAARW  186


>gi|308370243|ref|ZP_07420778.2| LOW QUALITY PROTEIN: enoyl-CoA hydratase echA18_1 [Mycobacterium 
tuberculosis SUMu002]
 gi|308324893|gb|EFP13744.1| LOW QUALITY PROTEIN: enoyl-CoA hydratase echA18_1 [Mycobacterium 
tuberculosis SUMu002]
Length=164

 Score =  326 bits (836),  Expect = 1e-87, Method: Compositional matrix adjust.
 Identities = 164/164 (100%), Positives = 164/164 (100%), Gaps = 0/164 (0%)

Query  50   ALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNT  109
            ALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNT
Sbjct  1    ALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNT  60

Query  110  RMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIATDDARFGI  169
            RMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIATDDARFGI
Sbjct  61   RMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIATDDARFGI  120

Query  170  PLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAARW  213
            PLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAARW
Sbjct  121  PLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAARW  164


>gi|289763556|ref|ZP_06522934.1| enoyl-CoA hydratase echA18 [Mycobacterium tuberculosis GM 1503]
 gi|289711062|gb|EFD75078.1| enoyl-CoA hydratase echA18 [Mycobacterium tuberculosis GM 1503]
Length=189

 Score =  299 bits (765),  Expect = 2e-79, Method: Compositional matrix adjust.
 Identities = 151/151 (100%), Positives = 151/151 (100%), Gaps = 0/151 (0%)

Query  63   LNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYNES  122
            LNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYNES
Sbjct  39   LNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYNES  98

Query  123  LAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIATDDARFGIPLGKLGVTTGFTE  182
            LAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIATDDARFGIPLGKLGVTTGFTE
Sbjct  99   LAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIATDDARFGIPLGKLGVTTGFTE  158

Query  183  ADTVARLIGPAALKYLLFSGELIGIEEAARW  213
            ADTVARLIGPAALKYLLFSGELIGIEEAARW
Sbjct  159  ADTVARLIGPAALKYLLFSGELIGIEEAARW  189


>gi|240172692|ref|ZP_04751351.1| putative enoyl-CoA hydratase [Mycobacterium kansasii ATCC 12478]
Length=294

 Score =  275 bits (702),  Expect = 4e-72, Method: Compositional matrix adjust.
 Identities = 148/208 (72%), Positives = 168/208 (81%), Gaps = 1/208 (0%)

Query  6    MTKMDEASNPCGGDIEAEMCQLMREQPPAEGVVDRVALQRHRNVALITLSHPQAQNALNL  65
            M   +E   P GGD+EA +  +MR +PPA  V+DRVA+Q HRNV + TL  PQA NALNL
Sbjct  1    MAPTEEIITP-GGDLEAVISAMMRSEPPAAQVIDRVAVQIHRNVVVTTLCKPQAHNALNL  59

Query  66   ASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYNESLAV  125
            A+WRRLK L DDLA ES LR VV+RGAGD AFAAGADI EFP TRM+A DAA YNES+A 
Sbjct  60   AAWRRLKLLFDDLARESDLRVVVIRGAGDAAFAAGADISEFPRTRMTAVDAATYNESVAA  119

Query  126  CLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIATDDARFGIPLGKLGVTTGFTEADT  185
            CLRA+T +P+PVI AVRGLAVGGGCEL  ACDV IA DDARFGIP+GKLGVT G TE+DT
Sbjct  120  CLRAVTALPVPVIGAVRGLAVGGGCELLAACDVRIAADDARFGIPIGKLGVTLGLTESDT  179

Query  186  VARLIGPAALKYLLFSGELIGIEEAARW  213
            VA LIGPAALKYLLFSGEL+ +E+AARW
Sbjct  180  VAGLIGPAALKYLLFSGELVDVEQAARW  207


>gi|324999509|ref|ZP_08120621.1| enoyl-CoA hydratase [Pseudonocardia sp. P1]
Length=286

 Score =  222 bits (566),  Expect = 3e-56, Method: Compositional matrix adjust.
 Identities = 121/190 (64%), Positives = 153/190 (81%), Gaps = 0/190 (0%)

Query  23   EMCQLMREQPPAEGVVDRVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGES  82
            E+ +++RE PPAE VVDRVA+Q   +VA++TLS P+A+NAL+LA+W+RL+ +  +L G  
Sbjct  9    ELGRVLREDPPAELVVDRVAVQDVDDVAVVTLSKPEARNALSLAAWQRLRTVFTELGGRD  68

Query  83   GLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVR  142
            GLRAVV+RGAG  A AAGADIKEFP TR+ AA A  YNES+A  LRA++ +P+PVIAA+ 
Sbjct  69   GLRAVVVRGAGPDALAAGADIKEFPQTRLGAAAATGYNESIARALRAVSAVPVPVIAAIH  128

Query  143  GLAVGGGCELATACDVCIATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSG  202
            GLAVGGGCELA ACDV IA+  ARFGIP+GKLGVT G+TE   VAR+IGPA LKYLLFSG
Sbjct  129  GLAVGGGCELAAACDVRIASTAARFGIPIGKLGVTLGYTETSAVARVIGPAELKYLLFSG  188

Query  203  ELIGIEEAAR  212
            +++G  +A R
Sbjct  189  DIVGAADAHR  198


>gi|221633735|ref|YP_002522961.1| enoyl-CoA hydratase [Thermomicrobium roseum DSM 5159]
 gi|221155424|gb|ACM04551.1| enoyl-CoA hydratase [Thermomicrobium roseum DSM 5159]
Length=261

 Score =  157 bits (398),  Expect = 7e-37, Method: Compositional matrix adjust.
 Identities = 83/172 (49%), Positives = 110/172 (64%), Gaps = 0/172 (0%)

Query  41   VALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAG  100
            + L+R   VA I L  P+  NAL  A W  L+ ++  LA +  +R V +RGAG++AF+AG
Sbjct  4    IRLERTGPVAHIVLDRPETLNALTAAMWHELRGIVRRLASDHSVRVVAIRGAGERAFSAG  63

Query  101  ADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCI  160
            ADI+EFP+ R   A A  Y+  ++  L A+   P PVIA +RGLAVGGG ELA ACD+ +
Sbjct  64   ADIREFPSRRTGLARARAYDRLVSAALAAIQEAPQPVIAVIRGLAVGGGLELAAACDIRL  123

Query  161  ATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAAR  212
            A  DARFG+P+G+LGV  G  E   + RL+ P  L  L+F GELI  EEA R
Sbjct  124  AAQDARFGLPIGRLGVMPGLGETRALLRLLPPGKLIELVFRGELIDAEEAWR  175


>gi|121534275|ref|ZP_01666099.1| Enoyl-CoA hydratase/isomerase [Thermosinus carboxydivorans Nor1]
 gi|121307045|gb|EAX47963.1| Enoyl-CoA hydratase/isomerase [Thermosinus carboxydivorans Nor1]
Length=261

 Score =  138 bits (347),  Expect = 6e-31, Method: Compositional matrix adjust.
 Identities = 74/164 (46%), Positives = 103/164 (63%), Gaps = 2/164 (1%)

Query  49   VALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPN  108
            + +IT++ P+A NALN A+ R L  LLD +A +  ++ V++ G+G+KAF AGADI E  +
Sbjct  15   IGIITINRPKALNALNGATMRELNELLDVIAQDPSVKVVIITGSGEKAFVAGADITEMQS  74

Query  109  TRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIATDDARFG  168
              MSA +   + +        L  +P PVIAAV G A+GGGCELA ACD+ IA+D A+FG
Sbjct  75   --MSAIEGRNWGKLGQAVFDKLENLPQPVIAAVNGFALGGGCELAMACDIRIASDKAKFG  132

Query  169  IPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAAR  212
             P   LG+T GF     + RL+G    K LLF+G++I   EA R
Sbjct  133  QPEVTLGITPGFAGTQRLPRLVGKGRAKELLFTGDMIDAAEAYR  176


>gi|344329229|gb|EGW40577.1| enoyl-CoA hydratase/isomerase family protein [Desulfosporosinus 
sp. OT]
Length=260

 Score =  137 bits (344),  Expect = 1e-30, Method: Compositional matrix adjust.
 Identities = 71/170 (42%), Positives = 110/170 (65%), Gaps = 2/170 (1%)

Query  43   LQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGAD  102
            L+++  VA++T++ P+A NALN  +   L  +LD+L  +S ++AV+L G+G+KAF AGAD
Sbjct  8    LEKNGAVAVLTINRPKALNALNSDTLAELSTVLDELGRDSSVKAVILTGSGEKAFVAGAD  67

Query  103  IKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIAT  162
            I +  +   +A +  ++ +      R L  +P PVIAA+ G A+GGGCELA ACD+ IA 
Sbjct  68   ISQMKD--FNALEGRQFAQLGQATFRKLELLPQPVIAAINGFALGGGCELAMACDIRIAG  125

Query  163  DDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAAR  212
            ++A+FG P   LG+T GF     + RL+G      LLF+G++I ++EA R
Sbjct  126  ENAKFGQPEVTLGLTAGFGGTQRLPRLVGTGLASELLFTGDIIDVQEAYR  175


>gi|254479680|ref|ZP_05092977.1| enoyl-CoA hydratase/isomerase family protein [Carboxydibrachium 
pacificum DSM 12653]
 gi|214034379|gb|EEB75156.1| enoyl-CoA hydratase/isomerase family protein [Carboxydibrachium 
pacificum DSM 12653]
Length=263

 Score =  137 bits (344),  Expect = 1e-30, Method: Compositional matrix adjust.
 Identities = 71/172 (42%), Positives = 108/172 (63%), Gaps = 2/172 (1%)

Query  41   VALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAG  100
            + ++  + +A IT++ P+A NALN  +   LK +L+ L  + G++ +V+ GAG+KAF AG
Sbjct  9    IEVKIEKGIATITINRPKALNALNTETLEELKNVLEVLQNDDGVKVIVITGAGEKAFVAG  68

Query  101  ADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCI  160
            ADI E  +  MS  +A ++ E      R +  M  PVIAAV G A+GGGCELA ACD+ I
Sbjct  69   ADISEMKD--MSVFEAKKFAELGQKVFRKIELMKKPVIAAVNGYALGGGCELALACDIRI  126

Query  161  ATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAAR  212
            A+ +A+FG P   LG+  GF     + R++G +  K L+++G++I  EEA R
Sbjct  127  ASRNAKFGQPEVGLGIIPGFGGTQRLPRIVGVSKAKELIYTGDMIDAEEALR  178


>gi|338814860|ref|ZP_08626835.1| enoyl-CoA hydratase/isomerase [Acetonema longum DSM 6540]
 gi|337273165|gb|EGO61827.1| enoyl-CoA hydratase/isomerase [Acetonema longum DSM 6540]
Length=260

 Score =  135 bits (339),  Expect = 6e-30, Method: Compositional matrix adjust.
 Identities = 69/169 (41%), Positives = 104/169 (62%), Gaps = 2/169 (1%)

Query  44   QRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADI  103
            ++   +  +TL+ P+A NALN A  + +  L+D +A +  ++ V++ G+G+K+F AGADI
Sbjct  9    EKDGGIGFVTLNRPKALNALNAALMKEISHLMDQIAADPTVQVVIITGSGEKSFVAGADI  68

Query  104  KEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIATD  163
             E     MSA +   + +        +  +P PVIAAV G A+GGGCELA ACD+ IA++
Sbjct  69   TEM--QPMSAVEGRNWGKFGQATFDKIEKLPQPVIAAVNGFALGGGCELAMACDIRIASE  126

Query  164  DARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAAR  212
             A+FG P   LG+T GF     ++RL+G    K LLF+G+LI  +EA R
Sbjct  127  KAKFGQPEVSLGITPGFGGTQRLSRLVGKGRAKELLFTGDLIDAQEAYR  175


>gi|150015211|ref|YP_001307465.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium beijerinckii NCIMB 
8052]
 gi|20162438|gb|AAM14582.1|AF494018_1 crotonase [Clostridium beijerinckii]
 gi|149901676|gb|ABR32509.1| Enoyl-CoA hydratase/isomerase [Clostridium beijerinckii NCIMB 
8052]
Length=261

 Score =  134 bits (338),  Expect = 6e-30, Method: Compositional matrix adjust.
 Identities = 70/173 (41%), Positives = 108/173 (63%), Gaps = 2/173 (1%)

Query  40   RVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAA  99
             V L++  ++A++T++ P+A NALN  + + L  +LDDL  ++ + AV++ GAG+K+F A
Sbjct  5    NVILEKEGHLAIVTINRPKALNALNSETLKDLNVVLDDLEADNNVYAVIVTGAGEKSFVA  64

Query  100  GADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVC  159
            GADI E  +  ++     E+        R L  +  PVIAA+ G A+GGGCELA +CD+ 
Sbjct  65   GADISEMKD--LNEEQGKEFGILGNNVFRRLEKLDKPVIAAISGFALGGGCELAMSCDIR  122

Query  160  IATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAAR  212
            IA+  A+FG P   LG+T GF     +AR++GP   K L+++ +LI  EEA R
Sbjct  123  IASVKAKFGQPEAGLGITPGFGGTQRLARIVGPGKAKELIYTCDLINAEEAYR  175


>gi|304317109|ref|YP_003852254.1| enoyl-CoA hydratase/isomerase [Thermoanaerobacterium thermosaccharolyticum 
DSM 571]
 gi|302778611|gb|ADL69170.1| Enoyl-CoA hydratase/isomerase [Thermoanaerobacterium thermosaccharolyticum 
DSM 571]
Length=259

 Score =  133 bits (335),  Expect = 1e-29, Method: Compositional matrix adjust.
 Identities = 67/174 (39%), Positives = 108/174 (63%), Gaps = 2/174 (1%)

Query  39   DRVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFA  98
            + V L +   +ALI ++ P+A NALN  + + L  +LD +  +  ++ +++ G+G+KAF 
Sbjct  4    NNVLLNKDDGIALIIINRPKALNALNYETLKELDSVLDIVENDKEIKVLIITGSGEKAFV  63

Query  99   AGADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDV  158
            AGADI E  N  M+  +A +++       R +  +  PVIAAV G A+GGGCEL+ ACD+
Sbjct  64   AGADIAEMSN--MTPLEAKKFSLYGQKVFRKIEMLSKPVIAAVNGFALGGGCELSMACDI  121

Query  159  CIATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAAR  212
             IA+ +A+FG P   LG+  GF+    + RLIG +  K L+F+G++I  +EA +
Sbjct  122  RIASKNAKFGQPEVGLGIIPGFSGTQRLPRLIGTSKAKELIFTGDMINSDEAYK  175


>gi|20807045|ref|NP_622216.1| 3-hydroxybutyryl-CoA dehydratase [Thermoanaerobacter tengcongensis 
MB4]
 gi|20515532|gb|AAM23820.1| Enoyl-CoA hydratase/carnithine racemase [Thermoanaerobacter tengcongensis 
MB4]
Length=260

 Score =  133 bits (334),  Expect = 2e-29, Method: Compositional matrix adjust.
 Identities = 69/172 (41%), Positives = 108/172 (63%), Gaps = 2/172 (1%)

Query  41   VALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAG  100
            + ++  + +A IT++ P+A NALN  +   L+ +L+ L  + G++ +V+ GAG+KAF AG
Sbjct  6    IEVKIEKGIATITINRPKALNALNTETLEELENVLEVLQNDDGVKVIVITGAGEKAFVAG  65

Query  101  ADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCI  160
            ADI E  +  MS  +A ++ E      R +  +  PVIAAV G A+GGGCELA ACD+ I
Sbjct  66   ADISEMKD--MSVFEAKKFAELGQKVFRKIELVKKPVIAAVNGYALGGGCELALACDIRI  123

Query  161  ATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAAR  212
            A+ +A+FG P   LG+  GF     + R++G +  K L+++G++I  EEA R
Sbjct  124  ASRNAKFGQPEVGLGIIPGFGGTQRLPRIVGVSKAKELIYTGDMIDAEEALR  175


>gi|119896971|ref|YP_932184.1| putative carnitinyl-CoA dehydratase [Azoarcus sp. BH72]
 gi|119669384|emb|CAL93297.1| putative Carnitinyl-CoA dehydratase [Azoarcus sp. BH72]
Length=260

 Score =  132 bits (333),  Expect = 2e-29, Method: Compositional matrix adjust.
 Identities = 72/172 (42%), Positives = 104/172 (61%), Gaps = 1/172 (0%)

Query  40   RVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAA  99
            ++ + R   VA ++LS+P   NA+N   WR+L+    +L  + G+R +VLRGAGD+AFAA
Sbjct  4    QILVDRGDAVATVSLSNPGKLNAVNAGMWRQLRAAFAELGADPGVRCIVLRGAGDEAFAA  63

Query  100  GADIKEFPNTRMSAADAAEYNESL-AVCLRALTTMPIPVIAAVRGLAVGGGCELATACDV  158
            G DI+EF   R +  DA  Y+E L A  L A+   P+P +AA+RG  VGGG E+A  CD+
Sbjct  64   GGDIEEFRTVRATVDDALHYHEELVAAALNAIRDCPVPTVAAIRGACVGGGLEIAGCCDL  123

Query  159  CIATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEA  210
             IA   +RFG P+ KLG +    E   +  L+GPA +  +L  G ++   EA
Sbjct  124  RIAGASSRFGAPINKLGFSMYPGEMAGLLALVGPAVVLEILLEGRILDAAEA  175


>gi|168186033|ref|ZP_02620668.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium botulinum C str. 
Eklund]
 gi|169295872|gb|EDS78005.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium botulinum C str. 
Eklund]
Length=261

 Score =  132 bits (333),  Expect = 3e-29, Method: Compositional matrix adjust.
 Identities = 72/173 (42%), Positives = 106/173 (62%), Gaps = 2/173 (1%)

Query  40   RVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAA  99
             V LQ+  ++A++T++ P+A NALN  +   L  +LD++  +  + AV++ GAG+KAF A
Sbjct  5    NVLLQKEGSLAIVTINRPKALNALNTETLNELDLVLDEIENDDNVYAVIVTGAGEKAFVA  64

Query  100  GADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVC  159
            GADI E  +   S  +  ++        R L T+  PVIAAV G A+GGGCEL+ ACD+ 
Sbjct  65   GADISEMKDK--SVLEGRKFGLLGNKIFRKLETLGKPVIAAVNGFALGGGCELSMACDIR  122

Query  160  IATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAAR  212
            IA++ A+FG P   LG+T GF     +ARL+G    K L++S + I  EEA R
Sbjct  123  IASEKAKFGQPEVSLGITPGFGGTQRLARLVGMGMAKELIYSAKNINAEEAFR  175


>gi|1903327|emb|CAB07495.1| crotonase [Thermoanaerobacterium thermosaccharolyticum DSM 571]
Length=259

 Score =  132 bits (332),  Expect = 3e-29, Method: Compositional matrix adjust.
 Identities = 66/174 (38%), Positives = 107/174 (62%), Gaps = 2/174 (1%)

Query  39   DRVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFA  98
            + V L +   +ALI ++ P+A NALN  + + L  +LD +  +  ++ +++ G+G+K F 
Sbjct  4    NNVLLNKDDGIALIIINRPKALNALNYETLKELDSVLDIVENDKEIKVLIITGSGEKTFV  63

Query  99   AGADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDV  158
            AGADI E  N  M+  +A +++       R +  +  PVIAAV G A+GGGCEL+ ACD+
Sbjct  64   AGADIAEMSN--MTPLEAKKFSLYGQKVFRKIEMLSKPVIAAVNGFALGGGCELSMACDI  121

Query  159  CIATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAAR  212
             IA+ +A+FG P   LG+  GF+    + RLIG +  K L+F+G++I  +EA +
Sbjct  122  RIASKNAKFGQPEVGLGIIPGFSGTQRLPRLIGTSKAKELIFTGDMINSDEAYK  175


>gi|121535320|ref|ZP_01667133.1| Enoyl-CoA hydratase/isomerase [Thermosinus carboxydivorans Nor1]
 gi|121306106|gb|EAX47035.1| Enoyl-CoA hydratase/isomerase [Thermosinus carboxydivorans Nor1]
Length=259

 Score =  132 bits (332),  Expect = 3e-29, Method: Compositional matrix adjust.
 Identities = 70/174 (41%), Positives = 104/174 (60%), Gaps = 2/174 (1%)

Query  39   DRVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFA  98
            D + ++R   +A+I ++ P+  NALNLA+   L R++  L  +  + A+++ G+G+KAF 
Sbjct  3    DNIVVERDGAIAVIIVNRPKEMNALNLATMLELDRIIRSLEDDQSVGALIITGSGEKAFV  62

Query  99   AGADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDV  158
            AGADI E  +  M A  A E+          +   P PVIAAV G A+GGGCELA ACD+
Sbjct  63   AGADISEMAD--MGAMQAKEWARLGQQVFSRIENFPRPVIAAVNGYALGGGCELAMACDI  120

Query  159  CIATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAAR  212
             +A++ A+FG P   LG+  GF     + RL+G    K L+FSG++I  +EA R
Sbjct  121  RLASEKAKFGQPEVNLGIIPGFGGTQRLTRLVGKGQAKLLIFSGDIIDAQEALR  174


>gi|150016904|ref|YP_001309158.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium beijerinckii NCIMB 
8052]
 gi|149903369|gb|ABR34202.1| Enoyl-CoA hydratase/isomerase [Clostridium beijerinckii NCIMB 
8052]
Length=261

 Score =  132 bits (331),  Expect = 4e-29, Method: Compositional matrix adjust.
 Identities = 69/173 (40%), Positives = 107/173 (62%), Gaps = 2/173 (1%)

Query  40   RVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAA  99
             V L++  ++A++T++ P+A NALN  + + L  +L+DL  +S +  V++ GAG+K+F A
Sbjct  5    NVILEKEGHLAIVTINRPKALNALNSETLKDLDAVLEDLEKDSNMYTVIVTGAGEKSFVA  64

Query  100  GADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVC  159
            GADI E  +  ++     E+        R L  +  PVIAA+ G A+GGGCELA +CD+ 
Sbjct  65   GADISEMKD--LNEEQGKEFGILGNNVFRRLERLDKPVIAAISGFALGGGCELAMSCDIR  122

Query  160  IATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAAR  212
            IA+  A+FG P   LG+T GF     +AR++GP   K L+++ +LI  EEA R
Sbjct  123  IASVKAKFGQPEAGLGITPGFGGTQRLARIVGPGKAKELIYTCDLINAEEAYR  175


>gi|118444510|ref|YP_878945.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium novyi NT]
 gi|118134966|gb|ABK62010.1| enoyl-CoA hydratase/isomerase family protein [Clostridium novyi 
NT]
Length=261

 Score =  131 bits (330),  Expect = 6e-29, Method: Compositional matrix adjust.
 Identities = 71/172 (42%), Positives = 107/172 (63%), Gaps = 2/172 (1%)

Query  41   VALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAG  100
            V LQ+  ++A++T++ P+A NALN  +   L  +LD++  +  + AV++ GAG+KAF AG
Sbjct  6    VLLQKEGSLAIVTINRPKALNALNTETLNELDLVLDEIENDDNVYAVIVTGAGEKAFVAG  65

Query  101  ADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCI  160
            ADI E  +   S  +A ++        R + T+  PVIAAV G A+GGGCEL+ +CD+ I
Sbjct  66   ADISEMKDK--SVLEARKFGLLGNRIFRKVETLGKPVIAAVNGFALGGGCELSMSCDIRI  123

Query  161  ATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAAR  212
            A++ A+FG P   LG+T GF     +ARL+G    K L++S + I  EEA R
Sbjct  124  ASEKAKFGQPEVSLGITPGFGGTQRLARLVGMGMAKELIYSAKNINAEEALR  175


>gi|339323428|ref|YP_004682322.1| CaiB/baiF CoA-transferase family protein [Cupriavidus necator 
N-1]
 gi|338170036|gb|AEI81090.1| enoyl-CoA hydratase EchA [Cupriavidus necator N-1]
Length=259

 Score =  130 bits (328),  Expect = 1e-28, Method: Compositional matrix adjust.
 Identities = 69/170 (41%), Positives = 101/170 (60%), Gaps = 0/170 (0%)

Query  41   VALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAG  100
            + ++R   VA ITL++P   NA++L+ W+ L   +  L+ +  LR VVLRGAGDKAFAAG
Sbjct  5    ILIERDAQVATITLNNPDKLNAMDLSMWQGLTEAIQGLSADDTLRCVVLRGAGDKAFAAG  64

Query  101  ADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCI  160
            ADI EF   R +AA A +Y E     +RA+     P IA ++G  VGGG E+A+ CD+ I
Sbjct  65   ADIAEFDTMRANAAQAQQYGEITNATMRAIAECRHPTIALIQGACVGGGLEIASMCDLRI  124

Query  161  ATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEA  210
              + +RFG+P+ +LG+   + E   +  L GPA    ++  G +    EA
Sbjct  125  CGESSRFGVPVNRLGLVMSYGELGGLVALAGPATALEIVLEGRVFDAAEA  174


>gi|254517612|ref|ZP_05129668.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium sp. 7_2_43FAA]
 gi|226911361|gb|EEH96562.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium sp. 7_2_43FAA]
Length=260

 Score =  130 bits (327),  Expect = 1e-28, Method: Compositional matrix adjust.
 Identities = 71/175 (41%), Positives = 105/175 (60%), Gaps = 2/175 (1%)

Query  38   VDRVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAF  97
            +  + L++  N+A++T++ P+A NALN  + + L  +L+DL  +S +  V+L GAG+KAF
Sbjct  3    LKNIILEKEENLAIVTINRPKALNALNSETLKDLDVVLEDLENDSNIYCVILTGAGEKAF  62

Query  98   AAGADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACD  157
             AGADI E  +  +S  +  E+        R L  +  PVIAAV G A+GGGCELA ACD
Sbjct  63   VAGADISEMKD--LSEDEGKEFGLLGNRVFRRLEKLNKPVIAAVSGFALGGGCELAMACD  120

Query  158  VCIATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAAR  212
            + IA++ ARF  P   LG+T GF     + RL+G    K L+++   +  EEA R
Sbjct  121  IRIASEKARFAQPEVGLGITPGFGGTQRLPRLVGEGKAKELIYTCATVKAEEALR  175


>gi|194289756|ref|YP_002005663.1| enoyl-CoA hydratase/isomerase [Cupriavidus taiwanensis LMG 19424]
 gi|193223591|emb|CAQ69596.1| putative Enoyl-CoA hydratase/isomerase [Cupriavidus taiwanensis 
LMG 19424]
Length=259

 Score =  130 bits (327),  Expect = 1e-28, Method: Compositional matrix adjust.
 Identities = 69/170 (41%), Positives = 101/170 (60%), Gaps = 0/170 (0%)

Query  41   VALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAG  100
            + ++R   VA ITL++P   NA++L+ W+ L   +  L+ +  LR VVLRGAGDKAFAAG
Sbjct  5    ILIERDAQVATITLNNPDKLNAMDLSMWQGLTEAIQGLSADDTLRCVVLRGAGDKAFAAG  64

Query  101  ADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCI  160
            ADI EF   R +AA A +Y E     +RA+     P IA ++G  VGGG E+A+ CD+ I
Sbjct  65   ADIAEFDTMRANAAQAQQYGEITNATMRAIAECRHPTIALIQGACVGGGLEIASMCDLRI  124

Query  161  ATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEA  210
                +RFG+P+ +LG+   + E   +  L GPA    ++  G +   +EA
Sbjct  125  CGRSSRFGVPVNRLGLVMSYGELGGLVALAGPATALEIVLEGRVFDADEA  174


>gi|71909622|ref|YP_287209.1| enoyl-CoA hydratase/isomerase [Dechloromonas aromatica RCB]
 gi|71849243|gb|AAZ48739.1| Enoyl-CoA hydratase/isomerase [Dechloromonas aromatica RCB]
Length=258

 Score =  130 bits (327),  Expect = 1e-28, Method: Compositional matrix adjust.
 Identities = 63/163 (39%), Positives = 101/163 (62%), Gaps = 0/163 (0%)

Query  48   NVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFP  107
             +A +TL++P+  NA+NL+ W++L   +  +  +S +R VV+RGAG+ AFAAG D++EF 
Sbjct  11   EIATLTLNNPEKLNAINLSMWQQLADRMGKITADSSIRCVVIRGAGNDAFAAGGDLEEFV  70

Query  108  NTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIATDDARF  167
              R +   A  Y++ +AV L A+   P P +A + G  +GGG E+A  CD  IA + +RF
Sbjct  71   TARTTLEQALHYHDQVAVALNAIADCPHPTLALISGACIGGGLEIAGVCDFRIAGESSRF  130

Query  168  GIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEA  210
            G P+ +LG +    E + + RL+GPA +K +L  G ++   EA
Sbjct  131  GAPINRLGFSMYPGEMEGLLRLVGPAVVKEILLEGRILHAREA  173


>gi|302875799|ref|YP_003844432.1| Enoyl-CoA hydratase/isomerase [Clostridium cellulovorans 743B]
 gi|307689234|ref|ZP_07631680.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium cellulovorans 743B]
 gi|302578656|gb|ADL52668.1| Enoyl-CoA hydratase/isomerase [Clostridium cellulovorans 743B]
Length=260

 Score =  129 bits (325),  Expect = 2e-28, Method: Compositional matrix adjust.
 Identities = 69/173 (40%), Positives = 105/173 (61%), Gaps = 2/173 (1%)

Query  40   RVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAA  99
             V L++  ++A++T++ P+A NALN  + + L     D+  ++ + AVVL GAG+KAF A
Sbjct  5    NVLLEKEGHIAVVTINRPKALNALNSDTLKDLDAAFTDIENDADIYAVVLTGAGEKAFVA  64

Query  100  GADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVC  159
            GADI E     M+      + +      R + T+  PVIAA+ G A+GGGCE++ ACD+ 
Sbjct  65   GADITEMK--EMTVIQGRTFGQLGNRVFRKIETLSKPVIAAINGFALGGGCEISMACDIR  122

Query  160  IATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAAR  212
            IA+  ARFG P   LG+T GF     +ARL+G    K L+++G+++  EEA R
Sbjct  123  IASTTARFGQPEVGLGITPGFGGTQRLARLVGMGMAKELIYTGKVVKAEEALR  175


>gi|187932888|ref|YP_001884608.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium botulinum B str. 
Eklund 17B]
 gi|187721041|gb|ACD22262.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium botulinum B str. 
Eklund 17B]
Length=260

 Score =  129 bits (325),  Expect = 2e-28, Method: Compositional matrix adjust.
 Identities = 66/173 (39%), Positives = 108/173 (63%), Gaps = 2/173 (1%)

Query  40   RVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAA  99
             V L++  ++A++T++ P+A NALN  + + L  +L++L  +S + AV+L GAG+K+F A
Sbjct  5    NVILEKEGHLAIVTINRPKALNALNSETLKDLDTVLENLENDSNIYAVILTGAGEKSFVA  64

Query  100  GADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVC  159
            GADI E  +  ++     E+ E        L  +  PVIAA+ G A+GGGCEL+ +CD+ 
Sbjct  65   GADISEMKD--LNEEQGREFGELGNKVFLRLENLDKPVIAAISGFALGGGCELSMSCDIR  122

Query  160  IATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAAR  212
            IA++ A+FG P   LG+T GF     +AR++G    K ++++ ++I  EEA R
Sbjct  123  IASEKAKFGQPEAGLGITPGFGGTQRLARIVGLGKAKEMIYTCDIIKAEEAYR  175


>gi|188027000|emb|CAQ53134.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium saccharobutylicum]
Length=261

 Score =  129 bits (325),  Expect = 2e-28, Method: Compositional matrix adjust.
 Identities = 66/173 (39%), Positives = 108/173 (63%), Gaps = 2/173 (1%)

Query  40   RVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAA  99
             V L++  ++A++T++ P+A NALN  + + L  +++DL  +S + +V+L GAG+K+F A
Sbjct  5    NVILEKEGHLAIVTINRPKALNALNSETLKDLDTVIEDLEKDSNIYSVILTGAGEKSFVA  64

Query  100  GADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVC  159
            GADI E  +  ++     E+        R L  +  PVIAA+ G A+GGGCELA +CD+ 
Sbjct  65   GADISEMKD--LNEQQGKEFGILGNNVFRRLEKLDKPVIAAISGFALGGGCELAMSCDIR  122

Query  160  IATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAAR  212
            IA++ A+FG P   LG+T GF     +AR++G    K ++++ ++I  EEA R
Sbjct  123  IASEKAKFGQPEAGLGITPGFGGTQRLARIVGLGKAKEMIYTCDIIKAEEAYR  175


>gi|338975489|ref|ZP_08630840.1| enoyl-CoA hydratase [Bradyrhizobiaceae bacterium SG-6C]
 gi|338231233|gb|EGP06372.1| enoyl-CoA hydratase [Bradyrhizobiaceae bacterium SG-6C]
Length=263

 Score =  129 bits (325),  Expect = 2e-28, Method: Compositional matrix adjust.
 Identities = 63/172 (37%), Positives = 99/172 (58%), Gaps = 0/172 (0%)

Query  39   DRVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFA  98
            D+V  ++  NV  +  ++P+ +NA++L  W     L+   A +  +R VVL GAGDKAF 
Sbjct  5    DKVISRKDGNVGYVIFNNPERRNAMSLEMWEACTALMTQYAQDESIRVVVLAGAGDKAFV  64

Query  99   AGADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDV  158
            AGADI +F + R S     +YN+++     ++   P P IA +RG  VGGG  +A+ CD+
Sbjct  65   AGADISKFGDERASEEGVRKYNDAVEAAYASVHEFPKPTIAMIRGFCVGGGMGIASCCDL  124

Query  159  CIATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEA  210
             IA++DARF +P  KLG+  G+     +  ++GP+  K + F+  L    EA
Sbjct  125  RIASEDARFAVPAAKLGLGYGYPGVKRLMDVVGPSFTKEIFFTARLFDAMEA  176


>gi|110803399|ref|YP_699562.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium perfringens SM101]
 gi|110683900|gb|ABG87270.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium perfringens SM101]
Length=260

 Score =  129 bits (325),  Expect = 2e-28, Method: Compositional matrix adjust.
 Identities = 65/175 (38%), Positives = 104/175 (60%), Gaps = 2/175 (1%)

Query  38   VDRVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAF  97
            ++ V  ++  N+ ++T++ P+A NALN  + + L   +D +  +  +  V+L GAGDKAF
Sbjct  3    LNNVIFEKEGNIGVLTINRPKALNALNSETLKDLDTAIDHIEKQDDIYVVILTGAGDKAF  62

Query  98   AAGADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACD  157
             AGADI E  +  ++  +  E+        R L  +  PVIAA+ G A+GGGCE++ ACD
Sbjct  63   VAGADISEMKD--LNEEEGKEFGLLGNKVFRRLENLDKPVIAAINGFALGGGCEISMACD  120

Query  158  VCIATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAAR  212
            + IAT  A+F  P   LG+T GF     + R++GP   K L+++G++I  +EA R
Sbjct  121  IRIATTKAKFAQPEVGLGITPGFGGTQRLPRIVGPGKAKELIYTGDMIKADEALR  175


>gi|144897474|emb|CAM74338.1| Enoyl-CoA hydratase/carnithine racemase [Magnetospirillum gryphiswaldense 
MSR-1]
Length=259

 Score =  129 bits (325),  Expect = 2e-28, Method: Compositional matrix adjust.
 Identities = 69/172 (41%), Positives = 96/172 (56%), Gaps = 0/172 (0%)

Query  39   DRVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFA  98
            D + + R   +A +T++ P   NALNL  WR+L     DL+ E  +RA+VLRGAG KAFA
Sbjct  3    DEILVSRDGAIATVTINRPDRMNALNLPVWRKLTEAFLDLSTECDVRAIVLRGAGTKAFA  62

Query  99   AGADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDV  158
             GADI+EF   R +A  A  Y+  +   L A+   P PVIA + G  VGGG ELA  CD+
Sbjct  63   PGADIEEFDTLRANADQAKAYDVIMRGALSAVKNCPQPVIAMIYGPCVGGGLELACCCDM  122

Query  159  CIATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEA  210
             +A    +FG+P+ K+ V   + E   +  + GPA    +L    +   EEA
Sbjct  123  RLAARSGKFGVPINKISVVMAYPEIAVIRAVAGPAVAAEILLEARIFDAEEA  174


>gi|225182057|ref|ZP_03735488.1| Enoyl-CoA hydratase/isomerase [Dethiobacter alkaliphilus AHT 
1]
 gi|225167270|gb|EEG76090.1| Enoyl-CoA hydratase/isomerase [Dethiobacter alkaliphilus AHT 
1]
Length=261

 Score =  129 bits (324),  Expect = 3e-28, Method: Compositional matrix adjust.
 Identities = 66/174 (38%), Positives = 107/174 (62%), Gaps = 2/174 (1%)

Query  39   DRVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFA  98
            + +  ++  ++A +T++ P+A NALN  + + L ++  +LA +  + AV+L GAG+KAF 
Sbjct  4    ENILFEKDGSIATVTINRPKALNALNADTLKELDQVFTELAADDTVSAVILTGAGEKAFV  63

Query  99   AGADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDV  158
            AGADI    +  ++A +A  + +        +  +P PVIAA+ G A+GGGCELA+ACD+
Sbjct  64   AGADISFMKD--LTAVEARAFAQLGQAAFNKIENLPQPVIAAINGFALGGGCELASACDI  121

Query  159  CIATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAAR  212
             +A++ A+FG P   LGV  GF     + RL+G    K LL++G++I   EA R
Sbjct  122  RLASESAKFGQPEVNLGVPPGFAGTQRLPRLVGKGRAKELLYTGDMIDAAEAYR  175


>gi|83310414|ref|YP_420678.1| enoyl-CoA hydratase/carnithine racemase [Magnetospirillum magneticum 
AMB-1]
 gi|82945255|dbj|BAE50119.1| Enoyl-CoA hydratase/carnithine racemase [Magnetospirillum magneticum 
AMB-1]
Length=259

 Score =  129 bits (324),  Expect = 3e-28, Method: Compositional matrix adjust.
 Identities = 68/173 (40%), Positives = 98/173 (57%), Gaps = 0/173 (0%)

Query  39   DRVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFA  98
            D + L R   +A +TL+ P   NALNL  WR L    + ++ +  +  V+LRGAG KAFA
Sbjct  3    DVIQLVREGAIATVTLNRPDRMNALNLPMWRGLAEAFETISADRSIHVVILRGAGTKAFA  62

Query  99   AGADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDV  158
             GADI+EF   R +A  A  Y+  +   L  +   P PVIAA+ G  VGGG ELA  CD+
Sbjct  63   PGADIEEFDTLRANAEQAKAYDLVMRKALDTVRACPQPVIAAIWGPCVGGGLELACCCDI  122

Query  159  CIATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAA  211
             ++    +FG+P+ K+ V   + E   + R+ GPAA   +L  G ++  +EAA
Sbjct  123  RLSAKSGKFGVPINKISVVMAYPELAQIRRVAGPAAALEILLEGRIMDADEAA  175


>gi|169347081|ref|ZP_02866023.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium perfringens C str. 
JGS1495]
 gi|169296764|gb|EDS78893.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium perfringens C str. 
JGS1495]
Length=260

 Score =  129 bits (324),  Expect = 3e-28, Method: Compositional matrix adjust.
 Identities = 65/175 (38%), Positives = 104/175 (60%), Gaps = 2/175 (1%)

Query  38   VDRVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAF  97
            ++ V  ++  N+ ++T++ P+A NALN  + + L   +D +  +  +  V+L GAGDKAF
Sbjct  3    LNNVIFEKEGNIGVLTINRPKALNALNSETLKDLDTAIDHIEKQDDIYVVILTGAGDKAF  62

Query  98   AAGADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACD  157
             AGADI E  +  ++  +  E+        R L  +  PVIAA+ G A+GGGCE++ ACD
Sbjct  63   VAGADIAEMKD--LNEEEGKEFGLLGNKVFRRLENLDKPVIAAINGFALGGGCEISMACD  120

Query  158  VCIATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAAR  212
            + IAT  A+F  P   LG+T GF     + R++GP   K L+++G++I  +EA R
Sbjct  121  IRIATTKAKFAQPEVGLGITPGFGGTQRLPRIVGPGKAKELIYTGDMIKADEALR  175


>gi|168216734|ref|ZP_02642359.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium perfringens NCTC 
8239]
 gi|182381211|gb|EDT78690.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium perfringens NCTC 
8239]
Length=260

 Score =  129 bits (324),  Expect = 3e-28, Method: Compositional matrix adjust.
 Identities = 65/175 (38%), Positives = 104/175 (60%), Gaps = 2/175 (1%)

Query  38   VDRVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAF  97
            ++ V  ++  N+ ++T++ P+A NALN  + + L   +D +  +  +  V+L GAGDKAF
Sbjct  3    LNNVIFEKEGNIGVLTINRPKALNALNSETLKDLDTAIDHIEKQDDIYVVILTGAGDKAF  62

Query  98   AAGADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACD  157
             AGADI E  +  ++  +  E+        R L  +  PVIAA+ G A+GGGCE++ ACD
Sbjct  63   VAGADIAEMKD--LNEEEGKEFGLLGNKVFRRLENLDKPVIAAINGFALGGGCEISMACD  120

Query  158  VCIATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAAR  212
            + IAT  A+F  P   LG+T GF     + R++GP   K L+++G++I  +EA R
Sbjct  121  IRIATTKAKFAQPEVGLGITPGFGGTQRLPRIVGPGKAKELIYTGDMIKADEALR  175


>gi|18311283|ref|NP_563217.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium perfringens str. 
13]
 gi|110799215|ref|YP_696975.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium perfringens ATCC 
13124]
 gi|168213212|ref|ZP_02638837.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium perfringens CPE 
str. F4969]
 gi|182625533|ref|ZP_02953304.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium perfringens D str. 
JGS1721]
 gi|18145966|dbj|BAB82007.1| 3-hydroxybutryl-CoA dehydratase [Clostridium perfringens str. 
13]
 gi|110673862|gb|ABG82849.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium perfringens ATCC 
13124]
 gi|170715197|gb|EDT27379.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium perfringens CPE 
str. F4969]
 gi|177909221|gb|EDT71686.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium perfringens D str. 
JGS1721]
Length=260

 Score =  129 bits (324),  Expect = 3e-28, Method: Compositional matrix adjust.
 Identities = 65/175 (38%), Positives = 104/175 (60%), Gaps = 2/175 (1%)

Query  38   VDRVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAF  97
            ++ V  ++  N+ ++T++ P+A NALN  + + L   +D +  +  +  V+L GAGDKAF
Sbjct  3    LNNVIFEKEGNIGVLTINRPKALNALNSETLKDLDTAIDHIEKQDDIYVVILTGAGDKAF  62

Query  98   AAGADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACD  157
             AGADI E  +  ++  +  E+        R L  +  PVIAA+ G A+GGGCE++ ACD
Sbjct  63   VAGADIAEMKD--LNEEEGKEFGLLGNKVFRRLENLDKPVIAAINGFALGGGCEISMACD  120

Query  158  VCIATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAAR  212
            + IAT  A+F  P   LG+T GF     + R++GP   K L+++G++I  +EA R
Sbjct  121  IRIATTKAKFAQPEVGLGITPGFGGTQRLPRIVGPGKAKELIYTGDMIKADEALR  175


>gi|311107586|ref|YP_003980439.1| enoyl-CoA hydratase [Achromobacter xylosoxidans A8]
 gi|310762275|gb|ADP17724.1| enoyl-CoA hydratase/isomerase family protein 20 [Achromobacter 
xylosoxidans A8]
Length=263

 Score =  129 bits (323),  Expect = 3e-28, Method: Compositional matrix adjust.
 Identities = 73/177 (42%), Positives = 104/177 (59%), Gaps = 2/177 (1%)

Query  35   EGVVDRVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGD  94
            + V  RV L+R  ++A +TLSHP   NA+ +  W  L+ +   +A +  LR V++RG G 
Sbjct  3    DNVAGRVLLEREGDLARVTLSHPGRLNAITVGMWHELRDVFTRVAQDDALRCVIVRGEGG  62

Query  95   KAFAAGADIKEFPNTRMSAADAAEYN-ESLAVCLRALTTMPIPVIAAVRGLAVGGGCELA  153
              FAAGADI+EFP  R   A    Y+ E LA  L+A+   P PV+A + G+ VGGG E+A
Sbjct  63   N-FAAGADIREFPAQRGDMAGVQRYHREVLAPALQAVALCPHPVVAQIEGVCVGGGLEIA  121

Query  154  TACDVCIATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEA  210
            + CD+ IA + ARFG+P+ +LG      E   +  L+G AA   +L  G + G EEA
Sbjct  122  SQCDLRIAGESARFGVPINRLGFPMAPDEMSGLLALVGRAATLAILLEGRVFGAEEA  178


>gi|332284823|ref|YP_004416734.1| enoyl-CoA hydratase [Pusillimonas sp. T7-7]
 gi|330428776|gb|AEC20110.1| enoyl-CoA hydratase [Pusillimonas sp. T7-7]
Length=259

 Score =  129 bits (323),  Expect = 4e-28, Method: Compositional matrix adjust.
 Identities = 60/172 (35%), Positives = 97/172 (57%), Gaps = 0/172 (0%)

Query  39   DRVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFA  98
            D + +Q    +  + L+ P   NA+    WRRL  +++ L+ +  +R +++RGAG+K+F+
Sbjct  3    DLILVQHDGPIVTVVLNRPDKLNAMTRPMWRRLGEVIEQLSADDAVRCIIMRGAGEKSFS  62

Query  99   AGADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDV  158
             G DI EF   R + A A EY   +   +RALT    P++A + G+ VGGG E+A  CD+
Sbjct  63   PGNDIAEFETERSNKAQAIEYGHVMHSAVRALTQCKHPLVAQIHGICVGGGLEIAALCDI  122

Query  159  CIATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEA  210
             I  + +RFG P+  LG+   + E +++ RL GP     +L  G + G  EA
Sbjct  123  RICGESSRFGAPIKNLGLVMAYPELESIVRLTGPVIALEILLEGRVFGAAEA  174


>gi|56477517|ref|YP_159106.1| enoyl-CoA hydratase/carnithine racemase [Aromatoleum aromaticum 
EbN1]
 gi|56313560|emb|CAI08205.1| predicted Enoyl-CoA hydratase/carnithine racemase [Aromatoleum 
aromaticum EbN1]
Length=260

 Score =  129 bits (323),  Expect = 4e-28, Method: Compositional matrix adjust.
 Identities = 71/172 (42%), Positives = 100/172 (59%), Gaps = 1/172 (0%)

Query  40   RVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAA  99
            R+       VA + LS+P   NA++ A W  L    + +A + GLR V+LRG GDKAFAA
Sbjct  4    RIVFSSGDGVATLVLSNPGKLNAIDSAMWIALHETFEGIAADDGLRCVILRGDGDKAFAA  63

Query  100  GADIKEFPNTRMSAADAAEYNESL-AVCLRALTTMPIPVIAAVRGLAVGGGCELATACDV  158
            G DI+EF   R +  DA  Y++ L A  L A+   P+P +AA++G  VGGG E+A  CD+
Sbjct  64   GGDIEEFLTVRATVDDALHYHDGLVAAALDAIRACPVPTVAAIQGACVGGGLEIAGCCDL  123

Query  159  CIATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEA  210
             IA + ARFG P+ +LG +    E   +  L+GPA L  +L  G ++   EA
Sbjct  124  RIAGESARFGAPINRLGFSMYPGEMAGLLDLVGPAVLLEILLEGRILTAREA  175


>gi|182419479|ref|ZP_02950731.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium butyricum 5521]
 gi|237666419|ref|ZP_04526404.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium butyricum E4 str. 
BoNT E BL5262]
 gi|182376810|gb|EDT74382.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium butyricum 5521]
 gi|237657618|gb|EEP55173.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium butyricum E4 str. 
BoNT E BL5262]
Length=261

 Score =  129 bits (323),  Expect = 4e-28, Method: Compositional matrix adjust.
 Identities = 67/175 (39%), Positives = 110/175 (63%), Gaps = 2/175 (1%)

Query  38   VDRVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAF  97
            ++ V L++  ++A++T++ P+A NALN A+ + L  +L+DL  ++ + AV+L GAG+K+F
Sbjct  3    LENVILEKEGHLAIVTINRPKALNALNSATLKDLDTVLEDLENDTNIYAVILTGAGEKSF  62

Query  98   AAGADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACD  157
             AGADI E  +  ++ A   E+ E        L  +  PVIAA++G A+GGGCE++ ACD
Sbjct  63   VAGADIAEMKD--LNEAQGKEFGELGNKVFLRLENLNKPVIAAIQGFALGGGCEISMACD  120

Query  158  VCIATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAAR  212
            + IA++ A FG P   LG+T GF     +AR++G    K ++++   I  +EA R
Sbjct  121  IRIASETALFGQPEVGLGITPGFGGTQRLARIVGLGKAKEMIYTARNIKADEAYR  175


>gi|134299635|ref|YP_001113131.1| enoyl-CoA hydratase/isomerase [Desulfotomaculum reducens MI-1]
 gi|134052335|gb|ABO50306.1| Enoyl-CoA hydratase/isomerase [Desulfotomaculum reducens MI-1]
Length=260

 Score =  129 bits (323),  Expect = 4e-28, Method: Compositional matrix adjust.
 Identities = 65/174 (38%), Positives = 107/174 (62%), Gaps = 2/174 (1%)

Query  39   DRVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFA  98
            + + L++  N+AL+T++ P+A NALN      L + +D ++ +  +  V+L+GAG+KAF 
Sbjct  4    NTILLEKEGNIALLTINRPKALNALNPEVLTELGQAVDAISADDEIFVVILKGAGEKAFV  63

Query  99   AGADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDV  158
            AGADI    +  M+A +   + +      + L  MP P+IAA+ G A+GGGCELA ACD+
Sbjct  64   AGADITAMKD--MTALEGRNFGQLGQEVFKKLEYMPKPIIAAINGFALGGGCELAMACDI  121

Query  159  CIATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAAR  212
             +A++ ++FG P   LG+T GF     + RL+G    K L+++ ++IG  +A R
Sbjct  122  RVASEKSKFGQPEVGLGITPGFAGTQRLPRLVGKGRAKELIYTADIIGAADAHR  175


>gi|302527841|ref|ZP_07280183.1| cyclohexa-1,5-dienecarbonyl-CoA hydratase [Streptomyces sp. AA4]
 gi|302436736|gb|EFL08552.1| cyclohexa-1,5-dienecarbonyl-CoA hydratase [Streptomyces sp. AA4]
Length=262

 Score =  129 bits (323),  Expect = 4e-28, Method: Compositional matrix adjust.
 Identities = 70/170 (42%), Positives = 99/170 (59%), Gaps = 1/170 (0%)

Query  36   GVVDRVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDK  95
            G+ D +   R   VA +T S P+  NA+    W  +  +  ++  +  L+ +VL GAG +
Sbjct  3    GMTDGLEFTREGAVARLTFSRPEKMNAITRGMWAAIPGVAAEVEADPELKVLVLTGAG-R  61

Query  96   AFAAGADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATA  155
             F+AGADI EF   R +A  AA+Y+E++   + ALT +  P IA V+G  +GGGC+L+ A
Sbjct  62   HFSAGADIGEFRELRSTAEAAAKYDEAVDRAVAALTALRKPTIAMVQGNCIGGGCQLSVA  121

Query  156  CDVCIATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELI  205
            CD   A DDARFGI   KLG+   F     +  L+GPA  KY L SGEL+
Sbjct  122  CDFRFAADDARFGITPAKLGIVYHFASTRQLVSLVGPAHAKYFLLSGELV  171


>gi|338195379|gb|EGO87669.1| 3-hydroxybutyryl-CoA dehydratase [Clostridium botulinum C str. 
Stockholm]
Length=206

 Score =  128 bits (322),  Expect = 4e-28, Method: Compositional matrix adjust.
 Identities = 69/173 (40%), Positives = 104/173 (61%), Gaps = 2/173 (1%)

Query  40   RVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAA  99
             V L++  N+A++T++ P+A NALN  +   L  ++D++A +  +  V+L GAG+KAF A
Sbjct  5    NVLLEKENNIAIVTINRPEALNALNTDTLNELDLVVDEVAKDDDILVVILTGAGEKAFVA  64

Query  100  GADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVC  159
            GADI E  +   +  +  ++        R + T+  PVIAAV G A+GGGCEL+ ACD+ 
Sbjct  65   GADISEMKDK--NVMEGRKFGLLGNKIFRKVETLEKPVIAAVNGFALGGGCELSMACDIR  122

Query  160  IATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAAR  212
            IA+  A+FG P   LG+T GF     +AR IG    K L++S + I  +EA R
Sbjct  123  IASQKAKFGQPEVSLGITPGFGGTQRLARHIGMGMAKELIYSAKNINADEAFR  175


>gi|333978267|ref|YP_004516212.1| 3-hydroxybutyryl-CoA dehydratase [Desulfotomaculum kuznetsovii 
DSM 6115]
 gi|333821748|gb|AEG14411.1| 3-hydroxybutyryl-CoA dehydratase [Desulfotomaculum kuznetsovii 
DSM 6115]
Length=260

 Score =  128 bits (322),  Expect = 4e-28, Method: Compositional matrix adjust.
 Identities = 66/174 (38%), Positives = 111/174 (64%), Gaps = 2/174 (1%)

Query  39   DRVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFA  98
            + + L+R  ++A++T++ PQ  N L+  +   +++ +D+L  +S +R +++ GAG+KAF 
Sbjct  4    NNILLERDGSLAVLTVNRPQVLNTLDAETLLEIEQAVDELEKDSSVRVIIITGAGEKAFV  63

Query  99   AGADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDV  158
            AGADI     ++++  +A E+ +     L  +  +  PVIAAV G A+GGGCELA ACD+
Sbjct  64   AGADIVFM--SKLTPFEAREFAQLGQRVLSKIEYLSKPVIAAVNGYALGGGCELAMACDI  121

Query  159  CIATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAAR  212
             +A+++A+FG P  KLG+  GF     + RL+ PA  K +LF+G++I  E A R
Sbjct  122  RVASENAKFGQPEVKLGLIAGFGGTQRLTRLVNPAIAKEILFTGDMIDAETAKR  175


>gi|227485263|ref|ZP_03915579.1| 3-hydroxybutyryl-CoA dehydratase [Anaerococcus lactolyticus ATCC 
51172]
 gi|227236723|gb|EEI86738.1| 3-hydroxybutyryl-CoA dehydratase [Anaerococcus lactolyticus ATCC 
51172]
Length=259

 Score =  128 bits (322),  Expect = 4e-28, Method: Compositional matrix adjust.
 Identities = 69/170 (41%), Positives = 102/170 (60%), Gaps = 3/170 (1%)

Query  41   VALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAG  100
            V  + + ++A IT++ P+A NALN      L  LLD++     LR  +L G G ++F AG
Sbjct  6    VKYEVNGHIAKITINRPEALNALNSEVLDELNTLLDEVRANKDLRVAILTGEG-RSFVAG  64

Query  101  ADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCI  160
            ADIKE   + ++  +A E+        R L ++PIPVIAA+ G A+GGGCE+A +CD  I
Sbjct  65   ADIKEM--STLTPVEAKEFGYKGLHVFRKLESLPIPVIAAINGFALGGGCEIAMSCDFRI  122

Query  161  ATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEA  210
            A+D A FG P   LG+T GF     + RL+GP   KYL+++ + I  ++A
Sbjct  123  ASDKALFGQPEVGLGITPGFAGTQRLQRLVGPGYAKYLIYTAKNIKADKA  172



Lambda     K      H
   0.321    0.135    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Effective search space used: 251148961304


  Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
    Posted date:  Sep 5, 2011  4:36 AM
  Number of letters in database: 5,219,829,388
  Number of sequences in database:  15,229,318



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40