BLAST Search Results

BLASTP 2.2.25+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,229,318 sequences; 5,219,829,388 total letters Query= Rv3536c Length=261 Score E Sequences producing significant alignments: (Bits) Value gi|15610672|ref|NP_218053.1| hydratase [Mycobacterium tuberculos... 520 7e-146 gi|167969150|ref|ZP_02551427.1| hypothetical hydratase [Mycobact... 501 5e-140 gi|183984991|ref|YP_001853282.1| 2-keto-4-pentenoate hydratase [... 452 3e-125 gi|118619282|ref|YP_907614.1| 2-keto-4-pentenoate hydratase [Myc... 451 7e-125 gi|240172825|ref|ZP_04751484.1| 2-keto-4-pentenoate hydratase [M... 444 5e-123 gi|289759692|ref|ZP_06519070.1| hydratase [Mycobacterium tubercu... 436 2e-120 gi|342861423|ref|ZP_08718071.1| 2-keto-4-pentenoate hydratase [M... 433 1e-119 gi|118464113|ref|YP_879905.1| 2-keto-4-pentenoate hydratase [Myc... 433 1e-119 gi|342862362|ref|ZP_08719002.1| 2-keto-4-pentenoate hydratase [M... 431 4e-119 gi|254822618|ref|ZP_05227619.1| 2-keto-4-pentenoate hydratase [M... 431 7e-119 gi|41406629|ref|NP_959465.1| hypothetical protein MAP0531 [Mycob... 430 1e-118 gi|296166565|ref|ZP_06848995.1| 2-oxopent-4-enoate hydratase [My... 429 3e-118 gi|333992321|ref|YP_004524935.1| 2-keto-4-pentenoate hydratase [... 413 1e-113 gi|108801624|ref|YP_641821.1| 4-oxalocrotonate decarboxylase [My... 399 2e-109 gi|126437608|ref|YP_001073299.1| 4-oxalocrotonate decarboxylase ... 399 2e-109 gi|118469647|ref|YP_890166.1| 2-keto-4-pentenoate hydratase [Myc... 397 8e-109 gi|145222112|ref|YP_001132790.1| 4-oxalocrotonate decarboxylase ... 396 2e-108 gi|120406184|ref|YP_956013.1| 4-oxalocrotonate decarboxylase [My... 392 3e-107 gi|54022429|ref|YP_116671.1| putative hydratase [Nocardia farcin... 385 5e-105 gi|169627727|ref|YP_001701376.1| hydratase/decarboxylase [Mycoba... 379 2e-103 gi|241992733|gb|ACS73884.1| HsaE [Rhodococcus ruber] 374 1e-101 gi|226363858|ref|YP_002781640.1| 2-hydroxypenta-2,4-dienoate hyd... 374 1e-101 gi|111021505|ref|YP_704477.1| 2-hydroxypentadienoate hydratase [... 372 2e-101 gi|226304228|ref|YP_002764186.1| 2-hydroxypenta-2,4-dienoate hyd... 364 8e-99 gi|238481727|gb|ACR43930.1| 2-hydroxypenta-2,4-dienoate hydratas... 363 1e-98 gi|343925859|ref|ZP_08765374.1| putative 2-hydroxypenta-2,4-dien... 341 5e-92 gi|269127678|ref|YP_003301048.1| 2-oxopent-4-enoate hydratase [T... 333 1e-89 gi|262200906|ref|YP_003272114.1| 2-oxopent-4-enoate hydratase [G... 333 2e-89 gi|226365269|ref|YP_002783052.1| 2-hydroxypenta-2,4-dienoate hyd... 315 3e-84 gi|111025803|ref|YP_708223.1| 2-hydroxypenta-2,4-dienoate hydrat... 313 2e-83 gi|21321306|dbj|BAB97166.1| 2-hydroxypenta-2,4-dienoate hydratas... 311 5e-83 gi|111022762|ref|YP_705734.1| 2-keto-4-pentenoate hydratase [Rho... 308 7e-82 gi|319948624|ref|ZP_08022748.1| 2-oxopent-4-enoate hydratase [Di... 299 3e-79 gi|289763714|ref|ZP_06523092.1| LOW QUALITY PROTEIN: hypothetica... 258 6e-67 gi|297154942|gb|ADI04654.1| 2-oxopent-4-enoate hydratase [Strept... 246 2e-63 gi|331698069|ref|YP_004334308.1| 2-oxopent-4-enoate hydratase [P... 245 4e-63 gi|83590612|ref|YP_430621.1| 4-oxalocrotonate decarboxylase [Moo... 241 1e-61 gi|302388959|ref|YP_003824780.1| 2-oxopent-4-enoate hydratase [T... 238 5e-61 gi|284041910|ref|YP_003392250.1| 2-oxopent-4-enoate hydratase [C... 238 6e-61 gi|333924103|ref|YP_004497683.1| 2-oxopent-4-enoate hydratase [D... 238 7e-61 gi|323702337|ref|ZP_08114002.1| 2-oxopent-4-enoate hydratase [De... 238 9e-61 gi|78042951|ref|YP_360112.1| hydratase/decarboxylase family prot... 235 4e-60 gi|297530341|ref|YP_003671616.1| 2-oxopent-4-enoate hydratase [G... 234 6e-60 gi|261419701|ref|YP_003253383.1| 2-oxopent-4-enoate hydratase [G... 233 2e-59 gi|78044351|ref|YP_360156.1| hydratase/decarboxylase family prot... 232 5e-59 gi|167784118|gb|AAZ76889.2| 2-hydroxypenta-2,4-dienoate-hydratas... 231 6e-59 gi|302547426|ref|ZP_07299768.1| 2-oxopent-4-enoate hydratase [St... 231 7e-59 gi|121534036|ref|ZP_01665862.1| 4-oxalocrotonate decarboxylase [... 231 1e-58 gi|239937272|dbj|BAH79101.1| 2-hydroxypenta-2,4-dienoate hydrata... 229 3e-58 gi|258654208|ref|YP_003203364.1| 2-oxopent-4-enoate hydratase [N... 229 4e-58 >gi|15610672|ref|NP_218053.1| hydratase [Mycobacterium tuberculosis H37Rv] gi|15843148|ref|NP_338185.1| 2-keto-4-pentenoate hydratase [Mycobacterium tuberculosis CDC1551] gi|31794712|ref|NP_857205.1| hydratase [Mycobacterium bovis AF2122/97] 75 more sequence titles Length=261 Score = 520 bits (1340), Expect = 7e-146, Method: Compositional matrix adjust. Identities = 261/261 (100%), Positives = 261/261 (100%), Gaps = 0/261 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH Sbjct 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA Sbjct 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR Sbjct 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA Sbjct 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 Query 241 RAGDEFVADFTGLGLVRLSFE 261 RAGDEFVADFTGLGLVRLSFE Sbjct 241 RAGDEFVADFTGLGLVRLSFE 261 >gi|167969150|ref|ZP_02551427.1| hypothetical hydratase [Mycobacterium tuberculosis H37Ra] Length=363 Score = 501 bits (1289), Expect = 5e-140, Method: Compositional matrix adjust. Identities = 252/255 (99%), Positives = 252/255 (99%), Gaps = 0/255 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH Sbjct 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA Sbjct 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR Sbjct 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA Sbjct 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 Query 241 RAGDEFVADFTGLGL 255 RAGDEFVADFT G Sbjct 241 RAGDEFVADFTRAGF 255 >gi|183984991|ref|YP_001853282.1| 2-keto-4-pentenoate hydratase [Mycobacterium marinum M] gi|183178317|gb|ACC43427.1| 2-keto-4-pentenoate hydratase [Mycobacterium marinum M] Length=261 Score = 452 bits (1162), Expect = 3e-125, Method: Compositional matrix adjust. Identities = 223/261 (86%), Positives = 243/261 (94%), Gaps = 0/261 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 ML D+TRD++AADLAQAERSR PI LT ++P+IDVVDAY+IQLINIR+RVAEG RVVGH Sbjct 1 MLADSTRDKIAADLAQAERSRQPITPLTPSYPDIDVVDAYQIQLINIRRRVAEGGRVVGH 60 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSSP+MQQMMGVDEPDYGHLLD+M+VFED PV+ + YL PRVEVEVGFILAADLPGA Sbjct 61 KVGLSSPVMQQMMGVDEPDYGHLLDNMRVFEDAPVRTANYLYPRVEVEVGFILAADLPGA 120 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 CTEDDVLAATEALVP+IELIDTRIKDWQIKICDTIADNASAAGFVLGAARV PA++DV+ Sbjct 121 DCTEDDVLAATEALVPSIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVSPAEVDVK 180 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 AIDA LTRNGE+ AEGRSDAVLGNPATAVAWLA KVE FGVRLRKGDIVLPGSCTFAV+A Sbjct 181 AIDAVLTRNGELAAEGRSDAVLGNPATAVAWLARKVEGFGVRLRKGDIVLPGSCTFAVDA 240 Query 241 RAGDEFVADFTGLGLVRLSFE 261 AGDEFVADFTGLG VRLSF+ Sbjct 241 NAGDEFVADFTGLGSVRLSFQ 261 >gi|118619282|ref|YP_907614.1| 2-keto-4-pentenoate hydratase [Mycobacterium ulcerans Agy99] gi|118571392|gb|ABL06143.1| 2-keto-4-pentenoate hydratase [Mycobacterium ulcerans Agy99] Length=314 Score = 451 bits (1159), Expect = 7e-125, Method: Compositional matrix adjust. Identities = 223/261 (86%), Positives = 243/261 (94%), Gaps = 0/261 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 ML D+TRD++AADLAQAERSR PI LT ++P+IDVVDAY+IQLINIR+RVAEG RVVGH Sbjct 54 MLADSTRDKIAADLAQAERSRQPITPLTPSYPDIDVVDAYQIQLINIRRRVAEGGRVVGH 113 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSSP+MQQMMGVDEPDYGHLLD+M+VFED PV+ + YL PRVEVEVGFILAADLPGA Sbjct 114 KVGLSSPVMQQMMGVDEPDYGHLLDNMRVFEDAPVRTANYLYPRVEVEVGFILAADLPGA 173 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 CTEDDVLAATEALVP+IELIDTRIKDWQIKICDTIADNASAAGFVLGAARV PA++DV+ Sbjct 174 DCTEDDVLAATEALVPSIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVSPAEVDVK 233 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 AIDA LTRNGE+ AEGRSDAVLGNPATAVAWLA KVE FGVRLRKGDIVLPGSCTFAV+A Sbjct 234 AIDAVLTRNGELAAEGRSDAVLGNPATAVAWLARKVEGFGVRLRKGDIVLPGSCTFAVDA 293 Query 241 RAGDEFVADFTGLGLVRLSFE 261 AGDEFVADFTGLG VRLSF+ Sbjct 294 NAGDEFVADFTGLGSVRLSFQ 314 >gi|240172825|ref|ZP_04751484.1| 2-keto-4-pentenoate hydratase [Mycobacterium kansasii ATCC 12478] Length=261 Score = 444 bits (1143), Expect = 5e-123, Method: Compositional matrix adjust. Identities = 221/261 (85%), Positives = 240/261 (92%), Gaps = 0/261 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 ML ATRDELAA+LAQAERSR+PI LTAAHP++DVVDAYEIQLINIRQRVAEGARV+GH Sbjct 1 MLTVATRDELAAELAQAERSREPIAPLTAAHPDMDVVDAYEIQLINIRQRVAEGARVLGH 60 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSS MQQMMGVDEPDYGHLLD+MQVFEDTPV+A+RYL PRVEVEVGFILA DLPGA Sbjct 61 KVGLSSKAMQQMMGVDEPDYGHLLDEMQVFEDTPVKAARYLYPRVEVEVGFILAGDLPGA 120 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 CTEDDVLAATE+LVPAIELIDTRI DW+I +CDTIADNAS+AGFVLGAARV PAD+DV+ Sbjct 121 DCTEDDVLAATESLVPAIELIDTRITDWKIALCDTIADNASSAGFVLGAARVSPADIDVK 180 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 AIDA L RNGEVVA GRSDAVLGNP TAVAWLA KV+SFGVRLR+GDIVLPGSCT A++A Sbjct 181 AIDATLIRNGEVVAAGRSDAVLGNPVTAVAWLARKVDSFGVRLRQGDIVLPGSCTRAIDA 240 Query 241 RAGDEFVADFTGLGLVRLSFE 261 AGDEFVA+F GLG VRLSFE Sbjct 241 HAGDEFVAEFAGLGSVRLSFE 261 >gi|289759692|ref|ZP_06519070.1| hydratase [Mycobacterium tuberculosis T85] gi|289715256|gb|EFD79268.1| hydratase [Mycobacterium tuberculosis T85] Length=218 Score = 436 bits (1120), Expect = 2e-120, Method: Compositional matrix adjust. Identities = 217/218 (99%), Positives = 218/218 (100%), Gaps = 0/218 (0%) Query 44 LINIRQRVAEGARVVGHKVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSP 103 +INIRQRVAEGARVVGHKVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSP Sbjct 1 MINIRQRVAEGARVVGHKVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSP 60 Query 104 RVEVEVGFILAADLPGAGCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAA 163 RVEVEVGFILAADLPGAGCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAA Sbjct 61 RVEVEVGFILAADLPGAGCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAA 120 Query 164 GFVLGAARVPPADLDVRAIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRL 223 GFVLGAARVPPADLDVRAIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRL Sbjct 121 GFVLGAARVPPADLDVRAIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRL 180 Query 224 RKGDIVLPGSCTFAVEARAGDEFVADFTGLGLVRLSFE 261 RKGDIVLPGSCTFAVEARAGDEFVADFTGLGLVRLSFE Sbjct 181 RKGDIVLPGSCTFAVEARAGDEFVADFTGLGLVRLSFE 218 >gi|342861423|ref|ZP_08718071.1| 2-keto-4-pentenoate hydratase [Mycobacterium colombiense CECT 3035] gi|342131323|gb|EGT84604.1| 2-keto-4-pentenoate hydratase [Mycobacterium colombiense CECT 3035] Length=261 Score = 433 bits (1114), Expect = 1e-119, Method: Compositional matrix adjust. Identities = 215/261 (83%), Positives = 236/261 (91%), Gaps = 0/261 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 ML RDELAADLAQAERS +PI LTAAHP+IDVVDAYEIQLINIRQRVAEGARV+GH Sbjct 1 MLSREVRDELAADLAQAERSGEPIAPLTAAHPDIDVVDAYEIQLINIRQRVAEGARVLGH 60 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSS +QQMMGVDEPDYGHLLD+MQ+FEDTPV+A+RYL PRVEVEVGF+L ADLPGA Sbjct 61 KVGLSSKAIQQMMGVDEPDYGHLLDEMQLFEDTPVKANRYLYPRVEVEVGFVLNADLPGA 120 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 GCTEDDVLAATEALVP+IELIDTRI DWQI++CDTIADNAS+AGFVLGAARV P D+DV+ Sbjct 121 GCTEDDVLAATEALVPSIELIDTRITDWQIELCDTIADNASSAGFVLGAARVSPQDIDVK 180 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 IDA L NGEVVAEGR+DAVLGNP TAVAWLA KV+ FGVRLRKGD+VLPGSCT A++A Sbjct 181 GIDAVLRCNGEVVAEGRTDAVLGNPVTAVAWLARKVDGFGVRLRKGDVVLPGSCTRAIDA 240 Query 241 RAGDEFVADFTGLGLVRLSFE 261 R GDEFVA+FTGLG VRLS E Sbjct 241 RPGDEFVAEFTGLGSVRLSLE 261 >gi|118464113|ref|YP_879905.1| 2-keto-4-pentenoate hydratase [Mycobacterium avium 104] gi|254773582|ref|ZP_05215098.1| 2-keto-4-pentenoate hydratase [Mycobacterium avium subsp. avium ATCC 25291] gi|118165400|gb|ABK66297.1| 2-keto-4-pentenoate hydratase [Mycobacterium avium 104] gi|336458419|gb|EGO37393.1| 2-keto-4-pentenoate hydratase [Mycobacterium avium subsp. paratuberculosis S397] Length=261 Score = 433 bits (1114), Expect = 1e-119, Method: Compositional matrix adjust. Identities = 215/261 (83%), Positives = 236/261 (91%), Gaps = 0/261 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 ML ATRDELAA+LAQAERS +PI LTAA+PEIDVVDAYEIQLINIRQRVAEGARV+GH Sbjct 1 MLSVATRDELAAELAQAERSGEPIPPLTAAYPEIDVVDAYEIQLINIRQRVAEGARVLGH 60 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSS +QQMMGVDEPDYGHLLDDMQ+FEDTPV+ +RYL PRVEVEVGFIL ADLPGA Sbjct 61 KVGLSSLAIQQMMGVDEPDYGHLLDDMQLFEDTPVKTNRYLYPRVEVEVGFILNADLPGA 120 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 GCTEDDV+AATEA VPAIELIDTRI DW+I++CDTIADNAS+AGFVLGAARV P D+D++ Sbjct 121 GCTEDDVMAATEAFVPAIELIDTRITDWKIELCDTIADNASSAGFVLGAARVSPQDIDIK 180 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 IDA L RNGEVVAEGR+DAVLGNP TAVAWLA KV+ FGVRLRKGD+VLPGSCT A++A Sbjct 181 GIDAVLRRNGEVVAEGRTDAVLGNPVTAVAWLARKVDGFGVRLRKGDVVLPGSCTKAIDA 240 Query 241 RAGDEFVADFTGLGLVRLSFE 261 GDEFVADF GLG VRLSFE Sbjct 241 HPGDEFVADFAGLGSVRLSFE 261 >gi|342862362|ref|ZP_08719002.1| 2-keto-4-pentenoate hydratase [Mycobacterium colombiense CECT 3035] gi|342130033|gb|EGT83367.1| 2-keto-4-pentenoate hydratase [Mycobacterium colombiense CECT 3035] Length=261 Score = 431 bits (1109), Expect = 4e-119, Method: Compositional matrix adjust. Identities = 214/261 (82%), Positives = 236/261 (91%), Gaps = 0/261 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 ML ATRDELAA+LAQAERS +PI LTAAHP+IDVVDAYEIQLINIRQRVAEGARV+GH Sbjct 1 MLSVATRDELAAELAQAERSGEPIAPLTAAHPDIDVVDAYEIQLINIRQRVAEGARVLGH 60 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSS +QQMMGVDEPDYGHLLD+MQ+FEDTPV+A+RYL PRVEVEVGF+L ADLPGA Sbjct 61 KVGLSSKAIQQMMGVDEPDYGHLLDEMQLFEDTPVKANRYLYPRVEVEVGFVLNADLPGA 120 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 GCTEDDVLAATEALVP+IELIDTRI DWQI++CDTIADNAS+AGFVLGAARV P D+DV+ Sbjct 121 GCTEDDVLAATEALVPSIELIDTRITDWQIELCDTIADNASSAGFVLGAARVSPQDIDVK 180 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 I+A L NGEVVAEGR+DAVLGNP TAVAWLA KV+ FGVRLRKGD+VLPGSCT A++A Sbjct 181 GIEAVLRCNGEVVAEGRTDAVLGNPVTAVAWLARKVDGFGVRLRKGDVVLPGSCTRAIDA 240 Query 241 RAGDEFVADFTGLGLVRLSFE 261 GD FVADF GLG VRLSFE Sbjct 241 HPGDNFVADFAGLGSVRLSFE 261 >gi|254822618|ref|ZP_05227619.1| 2-keto-4-pentenoate hydratase [Mycobacterium intracellulare ATCC 13950] Length=261 Score = 431 bits (1107), Expect = 7e-119, Method: Compositional matrix adjust. Identities = 215/261 (83%), Positives = 236/261 (91%), Gaps = 0/261 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 ML ATRDELAADLAQAERS +PI LTAA+P+IDVVDAYEIQLINIRQRVAEGARV+GH Sbjct 1 MLSVATRDELAADLAQAERSGEPIAPLTAAYPDIDVVDAYEIQLINIRQRVAEGARVLGH 60 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSS +QQMMGVDEPDYGHLLD+MQ+FEDTPV+A+RYL PRVEVEVGFIL ADLPGA Sbjct 61 KVGLSSKAIQQMMGVDEPDYGHLLDEMQLFEDTPVKANRYLYPRVEVEVGFILNADLPGA 120 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 GCTEDDVLAATEALVP+IELIDTRI DW+I++CDTIADNAS+AGFVLG ARV P D+DV+ Sbjct 121 GCTEDDVLAATEALVPSIELIDTRITDWKIELCDTIADNASSAGFVLGEARVSPRDIDVK 180 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 IDA L NGEVVAEGR+DAVLGNP TAVAWLA KV+ FGVRLRKGD+VLPGSCT A++A Sbjct 181 GIDAVLRCNGEVVAEGRTDAVLGNPVTAVAWLARKVDGFGVRLRKGDVVLPGSCTRAIDA 240 Query 241 RAGDEFVADFTGLGLVRLSFE 261 GDEFVADF GLG VRLSFE Sbjct 241 HPGDEFVADFAGLGSVRLSFE 261 >gi|41406629|ref|NP_959465.1| hypothetical protein MAP0531 [Mycobacterium avium subsp. paratuberculosis K-10] gi|41394978|gb|AAS02848.1| hypothetical protein MAP_0531 [Mycobacterium avium subsp. paratuberculosis K-10] Length=261 Score = 430 bits (1105), Expect = 1e-118, Method: Compositional matrix adjust. Identities = 214/261 (82%), Positives = 235/261 (91%), Gaps = 0/261 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 ML ATRDELAA+LAQAERS +PI LTAA+PEIDVVDAYEIQLINIRQRVAEGARV+GH Sbjct 1 MLSVATRDELAAELAQAERSGEPIPPLTAAYPEIDVVDAYEIQLINIRQRVAEGARVLGH 60 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSS +QQMMGVDEPDYGHLLDDMQ+FEDTPV+ +RYL PRVEVEVGFIL ADLPGA Sbjct 61 KVGLSSLAIQQMMGVDEPDYGHLLDDMQLFEDTPVKTNRYLYPRVEVEVGFILNADLPGA 120 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 GCTEDDV+AATEA VPAIELIDTRI DW+I++CDTIADNAS+AGFVLGAARV P D+D++ Sbjct 121 GCTEDDVMAATEAFVPAIELIDTRITDWKIELCDTIADNASSAGFVLGAARVSPQDIDIK 180 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 IDA L RNGEVVAEGR+DAVLGNP TAVAWLA KV+ FGVRLRKGD+VLPGSCT A++A Sbjct 181 GIDAVLRRNGEVVAEGRTDAVLGNPVTAVAWLARKVDGFGVRLRKGDVVLPGSCTKAIDA 240 Query 241 RAGDEFVADFTGLGLVRLSFE 261 GDEFVADF GLG V LSFE Sbjct 241 HPGDEFVADFAGLGSVCLSFE 261 >gi|296166565|ref|ZP_06848995.1| 2-oxopent-4-enoate hydratase [Mycobacterium parascrofulaceum ATCC BAA-614] gi|295898051|gb|EFG77627.1| 2-oxopent-4-enoate hydratase [Mycobacterium parascrofulaceum ATCC BAA-614] Length=261 Score = 429 bits (1102), Expect = 3e-118, Method: Compositional matrix adjust. Identities = 215/261 (83%), Positives = 234/261 (90%), Gaps = 0/261 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 ML ATRDELAADLAQAERSR+PI LTA HPEIDVVDAYEIQLINIRQRVAEGARV+GH Sbjct 1 MLSVATRDELAADLAQAERSREPIAPLTAVHPEIDVVDAYEIQLINIRQRVAEGARVLGH 60 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSS +QQMMGVDEPDYGHLLD+MQ+FEDT V+ASRYL PRVEVEVGF+L ADLPGA Sbjct 61 KVGLSSKAIQQMMGVDEPDYGHLLDEMQLFEDTRVKASRYLWPRVEVEVGFLLNADLPGA 120 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 CTE DVLAATEALVP+IELIDTRI DW+I++CDTIADNAS+AGFVLG ARV P D+DV+ Sbjct 121 ECTEGDVLAATEALVPSIELIDTRITDWKIELCDTIADNASSAGFVLGEARVAPRDIDVK 180 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 IDA L NGEVVA GR+DAVLGNP TAVAWLA KVE FGVRLRKGD+VLPGSCT A++A Sbjct 181 GIDAVLRCNGEVVATGRTDAVLGNPVTAVAWLARKVEGFGVRLRKGDLVLPGSCTRAIDA 240 Query 241 RAGDEFVADFTGLGLVRLSFE 261 AGD+FVADFTGLG VRLSFE Sbjct 241 HAGDDFVADFTGLGCVRLSFE 261 >gi|333992321|ref|YP_004524935.1| 2-keto-4-pentenoate hydratase [Mycobacterium sp. JDM601] gi|333488289|gb|AEF37681.1| 2-keto-4-pentenoate hydratase [Mycobacterium sp. JDM601] Length=261 Score = 413 bits (1062), Expect = 1e-113, Method: Compositional matrix adjust. Identities = 204/261 (79%), Positives = 226/261 (87%), Gaps = 0/261 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 ML RDELAA+LAQAERSR PI LTAAHP+IDVVDAYEIQLINIRQR+AEGARVVGH Sbjct 1 MLSAQIRDELAAELAQAERSRVPISPLTAAHPDIDVVDAYEIQLINIRQRIAEGARVVGH 60 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSS MQQMMGVDEPDYGHLL++MQVFEDTPV+AS YL PRVEVEV FIL ADLPGA Sbjct 61 KVGLSSEAMQQMMGVDEPDYGHLLNEMQVFEDTPVKASNYLYPRVEVEVAFILGADLPGA 120 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 CTE+ VLAATEA PAIELID+RI DW+IK+CDTIADNAS+AGFVLG RV P D+D++ Sbjct 121 DCTEEHVLAATEAFAPAIELIDSRITDWKIKLCDTIADNASSAGFVLGKQRVKPGDIDIK 180 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 IDA LT+NGEVVA+GRSDAVLGNP TAVAWLA KVESFGVRL+ GDIVLPG+ T A++ Sbjct 181 GIDASLTKNGEVVAKGRSDAVLGNPVTAVAWLARKVESFGVRLKAGDIVLPGTATRAIDV 240 Query 241 RAGDEFVADFTGLGLVRLSFE 261 GD+ VADF+GLG VRL FE Sbjct 241 HPGDDCVADFSGLGSVRLVFE 261 >gi|108801624|ref|YP_641821.1| 4-oxalocrotonate decarboxylase [Mycobacterium sp. MCS] gi|119870778|ref|YP_940730.1| 4-oxalocrotonate decarboxylase [Mycobacterium sp. KMS] gi|108772043|gb|ABG10765.1| 4-oxalocrotonate decarboxylase [Mycobacterium sp. MCS] gi|119696867|gb|ABL93940.1| 4-oxalocrotonate decarboxylase [Mycobacterium sp. KMS] Length=261 Score = 399 bits (1026), Expect = 2e-109, Method: Compositional matrix adjust. Identities = 202/261 (78%), Positives = 226/261 (87%), Gaps = 0/261 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 ML R++LAADLAQAERSR P+ LT HP+IDVVDAYEIQLINIRQ+VAEGARV+GH Sbjct 1 MLSSEVREQLAADLAQAERSRVPMKPLTDGHPDIDVVDAYEIQLINIRQKVAEGARVIGH 60 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSS MQ+MMGVDEPDYGHLLDDMQV+ED PV++S YL PRVEVEVGFILA DLPGA Sbjct 61 KVGLSSEAMQKMMGVDEPDYGHLLDDMQVYEDKPVKSSNYLYPRVEVEVGFILADDLPGA 120 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 GCTE+DVLAAT A PAIELIDTRI DW+IK+CDTIADNAS+AG+VLG ARV P D+D++ Sbjct 121 GCTEEDVLAATAAFAPAIELIDTRITDWKIKLCDTIADNASSAGWVLGEARVSPKDIDIK 180 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 +IDA LT NGE VAEGRSDAVLGNP TAVAWLA KVESFGVRL+ GDIVLPGSCT A++A Sbjct 181 SIDAVLTNNGETVAEGRSDAVLGNPVTAVAWLARKVESFGVRLKAGDIVLPGSCTRAIDA 240 Query 241 RAGDEFVADFTGLGLVRLSFE 261 G FVADF GLG V+L+FE Sbjct 241 PPGSHFVADFAGLGSVQLAFE 261 >gi|126437608|ref|YP_001073299.1| 4-oxalocrotonate decarboxylase [Mycobacterium sp. JLS] gi|126237408|gb|ABO00809.1| 4-oxalocrotonate decarboxylase [Mycobacterium sp. JLS] Length=261 Score = 399 bits (1025), Expect = 2e-109, Method: Compositional matrix adjust. Identities = 203/261 (78%), Positives = 226/261 (87%), Gaps = 0/261 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 ML R++LAADLAQAERSR P+ LT HP+IDVVDAYEIQLINIRQ+VAEGARV+GH Sbjct 1 MLSSEVREQLAADLAQAERSRVPMKPLTDGHPDIDVVDAYEIQLINIRQKVAEGARVIGH 60 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSS MQ+MMGVDEPDYGHLLDDMQV+ED PV++S YL PRVEVEVGFILA DLPGA Sbjct 61 KVGLSSEAMQKMMGVDEPDYGHLLDDMQVYEDKPVKSSNYLYPRVEVEVGFILADDLPGA 120 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 GCTE+DVLAAT A PAIELIDTRI DW+IK+CDTIADNAS+AG+VLG ARV P D+D+R Sbjct 121 GCTEEDVLAATAAFAPAIELIDTRITDWKIKLCDTIADNASSAGWVLGEARVSPKDVDIR 180 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 AI+A LT NGE VAEGRSDAVLGNP TAVAWLA KVESFGVRL+ GDIVLPGSCT A++A Sbjct 181 AIEAVLTNNGETVAEGRSDAVLGNPVTAVAWLARKVESFGVRLKAGDIVLPGSCTRAIDA 240 Query 241 RAGDEFVADFTGLGLVRLSFE 261 G FVADF GLG V+L+FE Sbjct 241 PPGSHFVADFAGLGSVQLAFE 261 >gi|118469647|ref|YP_890166.1| 2-keto-4-pentenoate hydratase [Mycobacterium smegmatis str. MC2 155] gi|118170934|gb|ABK71830.1| 2-keto-4-pentenoate hydratase [Mycobacterium smegmatis str. MC2 155] Length=261 Score = 397 bits (1020), Expect = 8e-109, Method: Compositional matrix adjust. Identities = 204/261 (79%), Positives = 224/261 (86%), Gaps = 0/261 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 ML D R LAADLAQAERSR PI LT +P+IDVVDAYEIQLINIRQR+AEGA+VVGH Sbjct 1 MLSDEVRARLAADLAQAERSRQPITPLTDGNPDIDVVDAYEIQLINIRQRIAEGAKVVGH 60 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSS MQ+MMGVDEPDYGHLLDDMQV+ED PV+++ YL PRVEVEVGFILA DLPGA Sbjct 61 KVGLSSKAMQEMMGVDEPDYGHLLDDMQVYEDQPVKSANYLYPRVEVEVGFILADDLPGA 120 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 GCTE+DVLAAT A PAIELIDTRI +WQIK+CDTIADNAS+AG+VLG ARV P D+D+R Sbjct 121 GCTEEDVLAATAAFAPAIELIDTRITNWQIKLCDTIADNASSAGWVLGEARVSPKDIDIR 180 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 IDA L NGEVVAEGRSDAVLGNP TAVAWLA KVESFGVRL+ GDIVLPGSCT A++A Sbjct 181 NIDAVLKNNGEVVAEGRSDAVLGNPVTAVAWLARKVESFGVRLKAGDIVLPGSCTRAIDA 240 Query 241 RAGDEFVADFTGLGLVRLSFE 261 AG +FVADF GLG V L FE Sbjct 241 PAGSDFVADFAGLGSVHLRFE 261 >gi|145222112|ref|YP_001132790.1| 4-oxalocrotonate decarboxylase [Mycobacterium gilvum PYR-GCK] gi|315442552|ref|YP_004075431.1| 2-keto-4-pentenoate hydratase [Mycobacterium sp. Spyr1] gi|145214598|gb|ABP44002.1| 4-oxalocrotonate decarboxylase [Mycobacterium gilvum PYR-GCK] gi|315260855|gb|ADT97596.1| 2-keto-4-pentenoate hydratase [Mycobacterium sp. Spyr1] Length=261 Score = 396 bits (1017), Expect = 2e-108, Method: Compositional matrix adjust. Identities = 203/261 (78%), Positives = 227/261 (87%), Gaps = 0/261 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 ML R++LAADLA+AE+SR PI LT AHP+IDVVDAYEIQLINIR+RVA+GAR++GH Sbjct 1 MLPAEVREKLAADLAEAEQSRKPISPLTDAHPDIDVVDAYEIQLINIRRRVADGARIIGH 60 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSS MQ+MMGVDEPDYGHLL++MQVFED PV + RYL PRVEVEVGFIL+ DLPGA Sbjct 61 KVGLSSEAMQKMMGVDEPDYGHLLEEMQVFEDVPVGSGRYLYPRVEVEVGFILSDDLPGA 120 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 GCTEDDVLAAT A PAIELIDTRI +WQIK+CDTIADNAS+AG+VLG ARV P D+D+R Sbjct 121 GCTEDDVLAATAAFAPAIELIDTRITNWQIKLCDTIADNASSAGWVLGEARVSPKDVDIR 180 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 AIDA LT NGEVVAEGRSDAVLGNP TAVAWLA KVE+FGVRL+ GDIVLPGSCT A++A Sbjct 181 AIDAVLTNNGEVVAEGRSDAVLGNPVTAVAWLARKVEAFGVRLKAGDIVLPGSCTRAIDA 240 Query 241 RAGDEFVADFTGLGLVRLSFE 261 G FVADFTGLG VRL FE Sbjct 241 PPGSRFVADFTGLGSVRLDFE 261 >gi|120406184|ref|YP_956013.1| 4-oxalocrotonate decarboxylase [Mycobacterium vanbaalenii PYR-1] gi|119959002|gb|ABM16007.1| 4-oxalocrotonate decarboxylase [Mycobacterium vanbaalenii PYR-1] Length=261 Score = 392 bits (1007), Expect = 3e-107, Method: Compositional matrix adjust. Identities = 202/261 (78%), Positives = 223/261 (86%), Gaps = 0/261 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 ML R++LAADLA+AERSR PI LT P IDVVDAYEIQLINIRQRVAEGARV+GH Sbjct 1 MLSVEVREKLAADLAEAERSRVPISPLTDGQPGIDVVDAYEIQLINIRQRVAEGARVIGH 60 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSS MQ+MM VDEPDYGHLLD+MQVFE+ V+++ YL PRVEVEVGFIL+ DLPGA Sbjct 61 KVGLSSEAMQKMMNVDEPDYGHLLDEMQVFENVAVKSANYLYPRVEVEVGFILSDDLPGA 120 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 GCTEDDVLAAT A PAIELIDTRI +WQIK+CDTIADNAS+AG+VLG ARV P D+D++ Sbjct 121 GCTEDDVLAATAAFAPAIELIDTRITNWQIKLCDTIADNASSAGWVLGEARVSPKDVDIK 180 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 IDA LT NGEVVAEGRSDAVLGNP TAVAWLA KVESFGVRL+ GDIVLPGSCT A++A Sbjct 181 NIDAVLTNNGEVVAEGRSDAVLGNPVTAVAWLARKVESFGVRLKAGDIVLPGSCTRAIDA 240 Query 241 RAGDEFVADFTGLGLVRLSFE 261 AG FVADFTGLG VRL FE Sbjct 241 PAGSHFVADFTGLGSVRLDFE 261 >gi|54022429|ref|YP_116671.1| putative hydratase [Nocardia farcinica IFM 10152] gi|54013937|dbj|BAD55307.1| putative hydratase [Nocardia farcinica IFM 10152] Length=261 Score = 385 bits (988), Expect = 5e-105, Method: Compositional matrix adjust. Identities = 196/260 (76%), Positives = 216/260 (84%), Gaps = 0/260 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 ML DA R ELA +L +AE R I L A HP+IDVVDAYEIQLINIR+R+ GARVVGH Sbjct 1 MLSDAVRTELADELWRAETERVAIDPLVARHPDIDVVDAYEIQLINIRRRLDTGARVVGH 60 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSS MQQMMGVDEPDYGHLL +M+VFED PV+A RYL PRVEVEVGF+L ADLPGA Sbjct 61 KVGLSSLAMQQMMGVDEPDYGHLLAEMEVFEDVPVEAGRYLFPRVEVEVGFVLGADLPGA 120 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 CTE DVLAAT A PAIELID+RIKDW I +CDTI+DNAS+AGFVLG RV PAD+D++ Sbjct 121 DCTEADVLAATVAYAPAIELIDSRIKDWNIGLCDTISDNASSAGFVLGPQRVAPADIDIK 180 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 AIDA LTRNGEVVAEGRSDAVLG+P AVAWLA KV SFGVRLRKGDIVLPGSCT A++A Sbjct 181 AIDAVLTRNGEVVAEGRSDAVLGDPVIAVAWLARKVASFGVRLRKGDIVLPGSCTRAIDA 240 Query 241 RAGDEFVADFTGLGLVRLSF 260 R GD F A+F GLG VRL F Sbjct 241 RPGDTFHAEFAGLGSVRLQF 260 >gi|169627727|ref|YP_001701376.1| hydratase/decarboxylase [Mycobacterium abscessus ATCC 19977] gi|169239694|emb|CAM60722.1| Probable hydratase/decarboxylase [Mycobacterium abscessus] Length=261 Score = 379 bits (973), Expect = 2e-103, Method: Compositional matrix adjust. Identities = 185/261 (71%), Positives = 216/261 (83%), Gaps = 0/261 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 ML RD +AADLA+AER+R I T HP+ DVVDAYEIQL+NIR R+A+GA+VVGH Sbjct 1 MLSSEVRDSIAADLAEAERTRVAIAPPTDTHPDFDVVDAYEIQLMNIRSRLADGAKVVGH 60 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSS MQ+MMGV EPDYGHLL DM++FED AS++L PRVEVEVGFILAADLPG Sbjct 61 KVGLSSEAMQKMMGVHEPDYGHLLADMELFEDVAASASKFLFPRVEVEVGFILAADLPGE 120 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 CTEDDVLAAT A VP+IE++D+RI +WQIKICDTIADNAS+AGFV+G RV P D+D++ Sbjct 121 DCTEDDVLAATAAYVPSIEVVDSRITNWQIKICDTIADNASSAGFVVGKQRVSPKDIDIK 180 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 +IDA LT NGE +AEGRSDAVLGNP T+VAWLA KV SFGVRLR GD+VLPGSCT A +A Sbjct 181 SIDAVLTCNGEKLAEGRSDAVLGNPVTSVAWLARKVASFGVRLRAGDVVLPGSCTRAFDA 240 Query 241 RAGDEFVADFTGLGLVRLSFE 261 GDEF+A+F GLG VRL+FE Sbjct 241 HPGDEFLAEFAGLGSVRLAFE 261 >gi|241992733|gb|ACS73884.1| HsaE [Rhodococcus ruber] Length=261 Score = 374 bits (959), Expect = 1e-101, Method: Compositional matrix adjust. Identities = 187/260 (72%), Positives = 211/260 (82%), Gaps = 0/260 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 ML R LA +L AER R P+ L A HP+IDVVDAYEIQLINIR+R+A GARVVGH Sbjct 1 MLPTQLRTRLADELEAAERDRTPVDPLVARHPDIDVVDAYEIQLINIRRRLAAGARVVGH 60 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSS MQQMMGVDEPDYGHLLDDMQVFED PV SRY PRVEVEV F+L DLPGA Sbjct 61 KVGLSSLAMQQMMGVDEPDYGHLLDDMQVFEDRPVDTSRYCFPRVEVEVAFVLGKDLPGA 120 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 C E+DVL AT A P+IELID+RIKDW+I + DTIADNAS+AG+VLG RV P+D+D++ Sbjct 121 DCAENDVLDATVAFAPSIELIDSRIKDWKIGLADTIADNASSAGWVLGTQRVAPSDIDIK 180 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 IDA LTRNGE +AEGRSDAVLGNP TAVAWLA KV+SFGVRL+ GDIVLPGSCT A++A Sbjct 181 GIDAVLTRNGERIAEGRSDAVLGNPVTAVAWLARKVDSFGVRLKAGDIVLPGSCTRAIDA 240 Query 241 RAGDEFVADFTGLGLVRLSF 260 R GDEF A+F GLG V L+F Sbjct 241 RPGDEFHAEFAGLGSVSLTF 260 >gi|226363858|ref|YP_002781640.1| 2-hydroxypenta-2,4-dienoate hydratase [Rhodococcus opacus B4] gi|226242347|dbj|BAH52695.1| 2-hydroxypenta-2,4-dienoate hydratase [Rhodococcus opacus B4] Length=261 Score = 374 bits (959), Expect = 1e-101, Method: Compositional matrix adjust. Identities = 188/261 (73%), Positives = 211/261 (81%), Gaps = 0/261 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 ML R ELA L AER R PIG LTA HP+IDVVDAYEIQLINIR+R+ +GA+VVGH Sbjct 1 MLSTQLRTELADRLDAAERGRAPIGPLTADHPDIDVVDAYEIQLINIRRRLQDGAKVVGH 60 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSS MQQMMGVDEPDYGHLL DM+VFED PV +R+ PRVEVEV F+L ADLPGA Sbjct 61 KVGLSSLAMQQMMGVDEPDYGHLLADMEVFEDQPVDTARFCFPRVEVEVAFVLGADLPGA 120 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 GCTE DVL AT A P+IELID+RI DW+I + DTIADNAS+AGFVLG RV PAD+D++ Sbjct 121 GCTEQDVLDATVAFAPSIELIDSRITDWKITLPDTIADNASSAGFVLGKNRVRPADIDIK 180 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 AIDA LT NGE AEGRSDAVLGNP TAVAWLA KVESFGVRL+ GDIVLPGSCT A++A Sbjct 181 AIDAVLTSNGETKAEGRSDAVLGNPVTAVAWLAQKVESFGVRLKAGDIVLPGSCTRAIDA 240 Query 241 RAGDEFVADFTGLGLVRLSFE 261 GD F A+F+GLG V L F+ Sbjct 241 HPGDHFRAEFSGLGTVSLQFK 261 >gi|111021505|ref|YP_704477.1| 2-hydroxypentadienoate hydratase [Rhodococcus jostii RHA1] gi|110821035|gb|ABG96319.1| 2-hydroxypentadienoate hydratase [Rhodococcus jostii RHA1] Length=261 Score = 372 bits (956), Expect = 2e-101, Method: Compositional matrix adjust. Identities = 186/261 (72%), Positives = 210/261 (81%), Gaps = 0/261 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 ML R ELA L AER R PIG LTA HP+IDVVDAYEIQLINIR+R+ +GA+VVGH Sbjct 1 MLSTQLRTELANQLDAAERGRAPIGPLTADHPDIDVVDAYEIQLINIRRRLQDGAKVVGH 60 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSS MQQMMGVDEPDYGHLL DM+VFED PV +R+ PRVEVEV F+L ADLPGA Sbjct 61 KVGLSSLAMQQMMGVDEPDYGHLLADMEVFEDQPVDTARFCFPRVEVEVAFVLGADLPGA 120 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 GCTE DVL AT A P+IELID+RI DW+I + DTIADNAS+AGFVLG RV P D+D++ Sbjct 121 GCTEQDVLDATVAFAPSIELIDSRITDWKITLPDTIADNASSAGFVLGKNRVRPEDIDIK 180 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 +IDA LT NGE AEGRSDAVLGNP TAVAWLA KVESFGVRL+ GDIVLPGSCT A++A Sbjct 181 SIDAVLTSNGETKAEGRSDAVLGNPVTAVAWLAQKVESFGVRLKAGDIVLPGSCTRAIDA 240 Query 241 RAGDEFVADFTGLGLVRLSFE 261 GD F A+F+GLG V L F+ Sbjct 241 HPGDHFRAEFSGLGSVSLQFK 261 >gi|226304228|ref|YP_002764186.1| 2-hydroxypenta-2,4-dienoate hydratase [Rhodococcus erythropolis PR4] gi|229494163|ref|ZP_04387926.1| 2-hydroxypentadienoate hydratase [Rhodococcus erythropolis SK121] gi|158605229|gb|ABW74857.1| 2-hydroxypentadienoate hydratase [Rhodococcus erythropolis] gi|226183343|dbj|BAH31447.1| 2-hydroxypenta-2,4-dienoate hydratase [Rhodococcus erythropolis PR4] gi|229318525|gb|EEN84383.1| 2-hydroxypentadienoate hydratase [Rhodococcus erythropolis SK121] Length=261 Score = 364 bits (934), Expect = 8e-99, Method: Compositional matrix adjust. Identities = 183/260 (71%), Positives = 209/260 (81%), Gaps = 0/260 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 ML R ELA L AER R PI L A +P+IDVVDAYEIQLINIR+RVA GA+VVGH Sbjct 1 MLSTQIRGELADHLDSAERDRAPISPLIATYPDIDVVDAYEIQLINIRRRVAGGAKVVGH 60 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSS MQQMMGVDEPDYGHLL +M+ FED PV SR+ PRVEVEV FIL ADLPGA Sbjct 61 KVGLSSLAMQQMMGVDEPDYGHLLAEMEEFEDRPVDTSRFCFPRVEVEVAFILGADLPGA 120 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 GCTE DV+ AT A P+IELID+RIKDW+I + DTIADNAS+AGF+LG RV P+D+D++ Sbjct 121 GCTEQDVIDATVAYAPSIELIDSRIKDWKIGLSDTIADNASSAGFILGKERVRPSDIDIK 180 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 AIDA LT NGE +AEGRSDAVLGNP TAVAWLA KV++FGVRL+ GDIVLPGSCT A++A Sbjct 181 AIDAVLTCNGERIAEGRSDAVLGNPVTAVAWLAQKVDTFGVRLKAGDIVLPGSCTRAIDA 240 Query 241 RAGDEFVADFTGLGLVRLSF 260 GD F A+F+GLG V L F Sbjct 241 HPGDNFRAEFSGLGSVSLQF 260 >gi|238481727|gb|ACR43930.1| 2-hydroxypenta-2,4-dienoate hydratase [Rhodococcus sp. CECT 3014] Length=261 Score = 363 bits (933), Expect = 1e-98, Method: Compositional matrix adjust. Identities = 183/260 (71%), Positives = 209/260 (81%), Gaps = 0/260 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 ML R ELA L AER R PI L A +P+IDVVDAYEIQLINIR+RVA GA+VVGH Sbjct 1 MLSTQIRGELADHLDSAERDRAPISPLIATYPDIDVVDAYEIQLINIRRRVAGGAKVVGH 60 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSS MQQMMGVDEPDYGHLL +M+ FED PV SR+ PRVEVEV FIL ADLPGA Sbjct 61 KVGLSSLAMQQMMGVDEPDYGHLLAEMEEFEDRPVDTSRFCFPRVEVEVAFILGADLPGA 120 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 GCTE DV+ AT A P+IELID+RIKDW+I + DTIADNAS+AGF+LG RV P+D+D++ Sbjct 121 GCTEQDVIDATVAYAPSIELIDSRIKDWKIGLSDTIADNASSAGFILGKERVRPSDVDIK 180 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 AIDA LT NGE +AEGRSDAVLGNP TAVAWLA KV++FGVRL+ GDIVLPGSCT A++A Sbjct 181 AIDAVLTCNGERIAEGRSDAVLGNPVTAVAWLAQKVDTFGVRLKAGDIVLPGSCTRAIDA 240 Query 241 RAGDEFVADFTGLGLVRLSF 260 GD F A+F+GLG V L F Sbjct 241 HPGDNFRAEFSGLGSVSLQF 260 >gi|343925859|ref|ZP_08765374.1| putative 2-hydroxypenta-2,4-dienoate hydratase [Gordonia alkanivorans NBRC 16433] gi|343764210|dbj|GAA12300.1| putative 2-hydroxypenta-2,4-dienoate hydratase [Gordonia alkanivorans NBRC 16433] Length=262 Score = 341 bits (875), Expect = 5e-92, Method: Compositional matrix adjust. Identities = 164/254 (65%), Positives = 204/254 (81%), Gaps = 0/254 (0%) Query 7 RDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGHKVGLSS 66 R+++AA+L QAE S I + T +HP++DVVDAYEIQLINIR+R+ GARV GHKVGL+S Sbjct 8 REQIAAELWQAEVSATAIDRPTDSHPDLDVVDAYEIQLINIRKRLEAGARVAGHKVGLAS 67 Query 67 PIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGAGCTEDD 126 MQ+MMGVDEPDYGHLLD+MQ FE+TP+ A + PRVEVEVGFIL DLPG GCT +D Sbjct 68 EAMQKMMGVDEPDYGHLLDEMQYFEETPIDAKKLCFPRVEVEVGFILGEDLPGEGCTNED 127 Query 127 VLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVRAIDAKL 186 V+AA E +VP+IELID+RIKDW+I +CDTIADNAS+ G++LG RVP ++D IDA L Sbjct 128 VIAAVEWVVPSIELIDSRIKDWKITLCDTIADNASSCGWILGQQRVPIGEIDTGDIDAVL 187 Query 187 TRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEARAGDEF 246 RNGE+VA+G S AVLG+P AV+WLA KV+SFGVRLRKGD++LPG+ T A++ +GD F Sbjct 188 HRNGEIVAKGNSSAVLGHPLNAVSWLARKVDSFGVRLRKGDVILPGTATRAIDISSGDHF 247 Query 247 VADFTGLGLVRLSF 260 VA+F GLG V L F Sbjct 248 VAEFAGLGSVTLDF 261 >gi|269127678|ref|YP_003301048.1| 2-oxopent-4-enoate hydratase [Thermomonospora curvata DSM 43183] gi|268312636|gb|ACY99010.1| 2-oxopent-4-enoate hydratase [Thermomonospora curvata DSM 43183] Length=260 Score = 333 bits (854), Expect = 1e-89, Method: Compositional matrix adjust. Identities = 171/261 (66%), Positives = 200/261 (77%), Gaps = 1/261 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 ML + R E A L AE R PI LT ++P ID VDAYEIQLINIR+R+AEGA V GH Sbjct 1 MLTEEQRREAAGRLRDAELQRAPIPPLTESYPGIDAVDAYEIQLINIRRRLAEGAVVKGH 60 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSS MQQMMGVDEPDYGHLL DM++ D PV+ +RY PRVEVEVGFIL ADLPG Sbjct 61 KVGLSSKAMQQMMGVDEPDYGHLLGDMELRSDEPVEVARYCYPRVEVEVGFILGADLPGE 120 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 CTE DVLAATE +VPA+ELID+RI DW+I + DTIADNAS+AG+V+G V P LD+ Sbjct 121 TCTEADVLAATERVVPALELIDSRIIDWKIALADTIADNASSAGYVIGEG-VSPQGLDLT 179 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 AI+A L R+GE V GRSDAVLGNP T+VAWLA KV SFGVRL+ G +VLPGSCT A++ Sbjct 180 AIEAALYRDGEFVVSGRSDAVLGNPVTSVAWLARKVASFGVRLQAGHLVLPGSCTRAIDV 239 Query 241 RAGDEFVADFTGLGLVRLSFE 261 AG F A+F+GLG V L+F+ Sbjct 240 AAGQTFTAEFSGLGAVSLTFK 260 >gi|262200906|ref|YP_003272114.1| 2-oxopent-4-enoate hydratase [Gordonia bronchialis DSM 43247] gi|262084253|gb|ACY20221.1| 2-oxopent-4-enoate hydratase [Gordonia bronchialis DSM 43247] Length=262 Score = 333 bits (853), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 163/256 (64%), Positives = 200/256 (79%), Gaps = 0/256 (0%) Query 5 ATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGHKVGL 64 A R+++AADL QAE + I + T AHP++DVVDAYEI LINIR+R+ GA+V GHKVGL Sbjct 6 AIREQIAADLWQAEVTAVAIDRPTDAHPDVDVVDAYEIALINIRKRLDAGAKVAGHKVGL 65 Query 65 SSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGAGCTE 124 +S MQ+MM VDEPDYGHLLD+MQ FE TP+ S+ PRVEVEVGFIL ADLPG GCT Sbjct 66 ASEAMQKMMNVDEPDYGHLLDEMQYFESTPIDRSKLCYPRVEVEVGFILGADLPGEGCTN 125 Query 125 DDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVRAIDA 184 +DV+ A E +VP+IELID+RIKDW+I +CDTIADNAS+ G++LG RVP + +D IDA Sbjct 126 EDVIEAVEWVVPSIELIDSRIKDWKITLCDTIADNASSCGWILGEQRVPISAIDTGDIDA 185 Query 185 KLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEARAGD 244 + RNGEVVA+G S AVLG+P AV+WLA KV FGVRLRKGD++LPG+ T A++ AGD Sbjct 186 TIHRNGEVVAKGNSSAVLGHPLNAVSWLARKVAGFGVRLRKGDVILPGTATRAIDIDAGD 245 Query 245 EFVADFTGLGLVRLSF 260 FVA+F GLG V L F Sbjct 246 HFVAEFAGLGSVTLDF 261 >gi|226365269|ref|YP_002783052.1| 2-hydroxypenta-2,4-dienoate hydratase [Rhodococcus opacus B4] gi|226243759|dbj|BAH54107.1| 2-hydroxypenta-2,4-dienoate hydratase [Rhodococcus opacus B4] Length=262 Score = 315 bits (808), Expect = 3e-84, Method: Compositional matrix adjust. Identities = 157/261 (61%), Positives = 191/261 (74%), Gaps = 0/261 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 ML DA R A L AER R PI LT P+IDVVDAYEI L+NIR+R+A G V GH Sbjct 2 MLSDAQRLSAAHLLRNAERDRAPIDPLTTVFPDIDVVDAYEIALLNIRERLAAGETVYGH 61 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSS +MQ+MMGVDEPDYGHLL M E TP+ +R+ PR+EVE+G++L LPG Sbjct 62 KVGLSSEVMQKMMGVDEPDYGHLLSSMVYAEGTPIDTARFCYPRIEVEIGYVLGETLPGE 121 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 GCTE DVLAATE +VP+IELID+RI DW+I + DTIADNAS+AG +LGA RV PA++D+ Sbjct 122 GCTEADVLAATEYIVPSIELIDSRITDWKIGLADTIADNASSAGVILGAGRVEPANVDIG 181 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 I A L R E +A G + AVLGNP AVAWLA KV SFGVRL G ++LPGSCT A++A Sbjct 182 GISAVLFRGDEEIARGEASAVLGNPTKAVAWLARKVASFGVRLEAGHVILPGSCTRAIDA 241 Query 241 RAGDEFVADFTGLGLVRLSFE 261 R GD F A+F+ +G V + F+ Sbjct 242 RPGDLFRAEFSDIGSVSVEFK 262 >gi|111025803|ref|YP_708223.1| 2-hydroxypenta-2,4-dienoate hydratase [Rhodococcus jostii RHA1] gi|110824782|gb|ABH00065.1| 2-hydroxypenta-2,4-dienoate hydratase [Rhodococcus jostii RHA1] Length=268 Score = 313 bits (802), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 162/267 (61%), Positives = 194/267 (73%), Gaps = 7/267 (2%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 M+ D R E A LA AE +R PI L A +P IDVVDAYEIQL+ IR+RV GAR+VGH Sbjct 1 MIDDQARQEAAEMLASAENTRQPIDPLVARYPGIDVVDAYEIQLLGIRRRVGRGARIVGH 60 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSS +MQ MM VDEPDYGHLLDDM + E P+ Y +PRVEVEVGFIL ADLPG+ Sbjct 61 KVGLSSKVMQDMMNVDEPDYGHLLDDMMLTESVPIAVRAYCAPRVEVEVGFILGADLPGS 120 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 GC EDDV+ AT A+VP+IELID+RI +WQIKI DTIADNAS+AG VLG R+ P+ +D+ Sbjct 121 GCVEDDVIRATTAIVPSIELIDSRIANWQIKIEDTIADNASSAGVVLGRNRLDPSSVDLT 180 Query 181 AIDAKLT------RNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSC 234 + ++ +A+GRSDAVLGNPAT+VAWLA KV +FGV LR GD+VLPGS Sbjct 181 DVGVEVHVADATDPTPRFLAKGRSDAVLGNPATSVAWLANKVSAFGVSLRAGDLVLPGSL 240 Query 235 TFAVEARAGDEFVADF-TGLGLVRLSF 260 T A+E GD F A F GLG+V +F Sbjct 241 TRALEVHPGDTFTASFGGGLGMVTATF 267 >gi|21321306|dbj|BAB97166.1| 2-hydroxypenta-2,4-dienoate hydratase [Rhodococcus sp. RHA1] Length=268 Score = 311 bits (798), Expect = 5e-83, Method: Compositional matrix adjust. Identities = 161/267 (61%), Positives = 193/267 (73%), Gaps = 7/267 (2%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 M+ D R E A LA AE +R PI L A +P IDVVDAYEIQL+ IR+RV GAR+VGH Sbjct 1 MIDDQARQEAAEMLASAENTRQPIDPLVARYPGIDVVDAYEIQLLGIRRRVGRGARIVGH 60 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSS +MQ MM VDEPDYGHL DDM + E P+ Y +PRVEVEVGFIL ADLPG+ Sbjct 61 KVGLSSKVMQDMMNVDEPDYGHLFDDMMLTESVPIAVRAYCAPRVEVEVGFILGADLPGS 120 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 GC EDDV+ AT A+VP+IELID+RI +WQIKI DTIADNAS+AG VLG R+ P+ +D+ Sbjct 121 GCVEDDVIRATTAIVPSIELIDSRIANWQIKIEDTIADNASSAGVVLGRNRLDPSSVDLT 180 Query 181 AIDAKLT------RNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSC 234 + ++ +A+GRSDAVLGNPAT+VAWLA KV +FGV LR GD+VLPGS Sbjct 181 DVGVEVHVADATDPTPRFLAKGRSDAVLGNPATSVAWLANKVSAFGVSLRAGDLVLPGSL 240 Query 235 TFAVEARAGDEFVADF-TGLGLVRLSF 260 T A+E GD F A F GLG+V +F Sbjct 241 TRALEVHPGDTFTASFGGGLGMVTATF 267 >gi|111022762|ref|YP_705734.1| 2-keto-4-pentenoate hydratase [Rhodococcus jostii RHA1] gi|110822292|gb|ABG97576.1| 2-keto-4-pentenoate hydratase [Rhodococcus jostii RHA1] Length=261 Score = 308 bits (788), Expect = 7e-82, Method: Compositional matrix adjust. Identities = 155/261 (60%), Positives = 187/261 (72%), Gaps = 0/261 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 ML DA R A L AE+ R PI LT P IDVVDAYEI LINIR R G V GH Sbjct 1 MLSDAQRLSAAHLLRNAEQDRAPIDPLTTVFPGIDVVDAYEIALINIRARREAGETVYGH 60 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGLSS +MQ+MMGVDEPDYGHLL M E TP+ +R+ PR+EVE+G++L LPG Sbjct 61 KVGLSSEVMQKMMGVDEPDYGHLLSSMVYTEGTPIDTARFCYPRIEVEIGYVLGETLPGE 120 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 GCTE+DVLAATE +VP+IELID+RI DW+I + DTIADNAS+AG +LGA R PA++D+ Sbjct 121 GCTEEDVLAATEYIVPSIELIDSRITDWKIGLADTIADNASSAGVILGAGRGEPANVDIG 180 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 I A L + E +A G + AVLGNP AVAWLA KV SFGVRL G ++LPGSCT A++A Sbjct 181 GISAVLFQGEEEIARGEASAVLGNPTKAVAWLARKVASFGVRLEAGHVILPGSCTRAIDA 240 Query 241 RAGDEFVADFTGLGLVRLSFE 261 R GD F ADF+ +G V + F+ Sbjct 241 RPGDVFRADFSDIGSVSVEFK 261 >gi|319948624|ref|ZP_08022748.1| 2-oxopent-4-enoate hydratase [Dietzia cinnamea P4] gi|319437705|gb|EFV92701.1| 2-oxopent-4-enoate hydratase [Dietzia cinnamea P4] Length=264 Score = 299 bits (765), Expect = 3e-79, Method: Compositional matrix adjust. Identities = 154/260 (60%), Positives = 195/260 (75%), Gaps = 0/260 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 ML + TR LA L AE PI LT HP++D DA+EIQLINIR+++ GA V GH Sbjct 4 MLTEETRATLAQRLWDAETDVSPIAPLTDDHPDMDADDAFEIQLINIRRKLDSGAVVTGH 63 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGL+S MQ+MMGVDEPDYGHLLD M+ E TP++A+R+ PRVEVEVGFIL AD+P Sbjct 64 KVGLASKAMQEMMGVDEPDYGHLLDTMEYPEGTPIEAARFCFPRVEVEVGFILGADIPPE 123 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 GC++++ AA E +VP+IELID+RI+DW+IK+ DTIADNAS+ G+V+G RV AD+DV Sbjct 124 GCSQEEAAAAIEWVVPSIELIDSRIRDWKIKLPDTIADNASSCGYVIGQQRVRLADIDVA 183 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 AI+A L +NGE +A GRSDAVLGNP +V WLA V FGVRLRKGD++LPG+ A++A Sbjct 184 AIEATLYKNGEFLASGRSDAVLGNPLNSVGWLASTVAKFGVRLRKGDVILPGTAIRAMDA 243 Query 241 RAGDEFVADFTGLGLVRLSF 260 GD F A+F GLG V ++F Sbjct 244 TPGDTFRAEFAGLGDVSMAF 263 >gi|289763714|ref|ZP_06523092.1| LOW QUALITY PROTEIN: hypothetical hydratase [Mycobacterium tuberculosis GM 1503] gi|289711220|gb|EFD75236.1| LOW QUALITY PROTEIN: hypothetical hydratase [Mycobacterium tuberculosis GM 1503] Length=155 Score = 258 bits (659), Expect = 6e-67, Method: Compositional matrix adjust. Identities = 131/131 (100%), Positives = 131/131 (100%), Gaps = 0/131 (0%) Query 131 TEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVRAIDAKLTRNG 190 TEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVRAIDAKLTRNG Sbjct 25 TEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVRAIDAKLTRNG 84 Query 191 EVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEARAGDEFVADF 250 EVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEARAGDEFVADF Sbjct 85 EVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEARAGDEFVADF 144 Query 251 TGLGLVRLSFE 261 TGLGLVRLSFE Sbjct 145 TGLGLVRLSFE 155 >gi|297154942|gb|ADI04654.1| 2-oxopent-4-enoate hydratase [Streptomyces bingchenggensis BCW-1] Length=264 Score = 246 bits (629), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 133/259 (52%), Positives = 169/259 (66%), Gaps = 0/259 (0%) Query 2 LRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGHK 61 + DAT E A L A +R P+ L +P + + DAYEIQL+ IR+RV GAR+ GHK Sbjct 1 MTDATLTEAADTLLAAYDTRVPVEPLVDKYPGLTLADAYEIQLVQIRRRVEAGARIKGHK 60 Query 62 VGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGAG 121 VGLSSP MQ+ MGV +PDYGHLLDDM E+ PV ASR+L PRVE E+ F+L L G G Sbjct 61 VGLSSPAMQRQMGVGQPDYGHLLDDMFWLENAPVPASRFLQPRVEPEIAFVLKKSLTGPG 120 Query 122 CTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVRA 181 T + AA + ++PA+ELID+RI+DW+I I DTIADNAS+ G VLG+ D+D+R Sbjct 121 VTVAEAAAAVDFVLPALELIDSRIRDWRIGIIDTIADNASSGGVVLGSTPARLTDVDLRL 180 Query 182 IDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEAR 241 L RNG++V G S AVLG+P T++ WLA V + GV L G +VLPGS T +V Sbjct 181 AGCTLHRNGDLVGTGASGAVLGSPLTSLVWLANTVGTRGVVLEAGHVVLPGSITASVPVA 240 Query 242 AGDEFVADFTGLGLVRLSF 260 AGD A + GLG V F Sbjct 241 AGDTVTAAYAGLGSVTARF 259 >gi|331698069|ref|YP_004334308.1| 2-oxopent-4-enoate hydratase [Pseudonocardia dioxanivorans CB1190] gi|326952758|gb|AEA26455.1| 2-oxopent-4-enoate hydratase [Pseudonocardia dioxanivorans CB1190] Length=269 Score = 245 bits (626), Expect = 4e-63, Method: Compositional matrix adjust. Identities = 133/253 (53%), Positives = 166/253 (66%), Gaps = 0/253 (0%) Query 9 ELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGHKVGLSSPI 68 +LAA L AE+ P+ ++ HP DV DAY IQL +R RVA+G V GHKVGL++ + Sbjct 10 QLAAQLWDAEQRSVPVEPISRRHPGADVDDAYRIQLEGVRLRVAQGDSVRGHKVGLTARV 69 Query 69 MQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGAGCTEDDVL 128 MQQ GVD PD+GHLL M E P+ ++PRVE E+ F+L L G G T DVL Sbjct 70 MQQQFGVDRPDFGHLLGSMFHPEGEPLPVDALIAPRVEPELAFVLDQPLQGPGVTVADVL 129 Query 129 AATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVRAIDAKLTR 188 AAT ++PA+E+ID+RI+DWQI I DT+ADNAS+A VLG R P A LD R I L R Sbjct 130 AATAFVLPALEIIDSRIEDWQIGIVDTVADNASSARVVLGGVRTPIAGLDPRLIGVVLRR 189 Query 189 NGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEARAGDEFVA 248 NGE+V G S AVLGNPA AVAWLA + G LR GD+++PG+CT AV+ R GD A Sbjct 190 NGEIVETGASGAVLGNPAQAVAWLANTIAPGGGVLRAGDLIMPGTCTRAVDMRPGDTVRA 249 Query 249 DFTGLGLVRLSFE 261 DF LG V ++F Sbjct 250 DFEHLGHVGVTFS 262 >gi|83590612|ref|YP_430621.1| 4-oxalocrotonate decarboxylase [Moorella thermoacetica ATCC 39073] gi|83573526|gb|ABC20078.1| 4-oxalocrotonate decarboxylase [Moorella thermoacetica ATCC 39073] Length=257 Score = 241 bits (614), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 122/238 (52%), Positives = 160/238 (68%), Gaps = 0/238 (0%) Query 23 PIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGHKVGLSSPIMQQMMGVDEPDYGH 82 PI LT +P++ V DAY IQL I ++A G++V+G K+GL+S MQQ++GV+EPDYGH Sbjct 19 PIEPLTGTYPDLTVEDAYRIQLAGIETKLARGSKVIGKKIGLTSKGMQQLLGVNEPDYGH 78 Query 83 LLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGAGCTEDDVLAATEALVPAIELID 142 LLD M + E P + L PRVE E+ FIL L G G T DV ATE ++PA E++D Sbjct 79 LLDSMLLLEGEPCRREELLWPRVEGELAFILKDPLKGPGVTIADVYRATEGIMPAFEIVD 138 Query 143 TRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVRAIDAKLTRNGEVVAEGRSDAVL 202 +RI+DW+IK+ DTIADNAS+A VLG+ VP DLD+R I L +NGE+V+ G AV Sbjct 139 SRIRDWKIKLPDTIADNASSARLVLGSRMVPIKDLDLRLIGMVLEKNGEMVSSGAGAAVW 198 Query 203 GNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEARAGDEFVADFTGLGLVRLSF 260 G+PA AVAWLA K+ +F + L G+I+L G+ T A A AGD F F GLG + L F Sbjct 199 GHPAAAVAWLANKLAAFDIALEAGEIILSGAVTAAENASAGDVFTVSFYGLGSLSLKF 256 >gi|302388959|ref|YP_003824780.1| 2-oxopent-4-enoate hydratase [Thermosediminibacter oceani DSM 16646] gi|302199587|gb|ADL07157.1| 2-oxopent-4-enoate hydratase [Thermosediminibacter oceani DSM 16646] Length=257 Score = 238 bits (608), Expect = 5e-61, Method: Compositional matrix adjust. Identities = 126/251 (51%), Positives = 170/251 (68%), Gaps = 0/251 (0%) Query 10 LAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGHKVGLSSPIM 69 +A +L +AE +R I LT+ +P + V DAY IQL + R+ +G RV+G K+GL+S M Sbjct 6 IATNLLEAEETRKAIEPLTSTYPNLTVEDAYRIQLAGVEMRLKQGRRVIGKKIGLTSKAM 65 Query 70 QQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGAGCTEDDVLA 129 Q ++GV EPDYGHLLD+M + E P + ++ PRVE E+ F+L L G G T DV Sbjct 66 QDLLGVKEPDYGHLLDNMLLLEGEPCRREDFIWPRVEGELAFVLKDTLKGPGVTIADVFR 125 Query 130 ATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVRAIDAKLTRN 189 ATE ++PAIE++D+RI+DW+I + DTIADNAS+A FVLG+ VP DLD+R + L +N Sbjct 126 ATEGVMPAIEIVDSRIRDWKISLADTIADNASSARFVLGSRMVPIKDLDLRLLGMVLEKN 185 Query 190 GEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEARAGDEFVAD 249 GEVV+ G AV G+PA AVAWLA K+ +F + L G+IVL G+ T AV+A AGD F Sbjct 186 GEVVSTGTGAAVWGHPAAAVAWLANKLAAFDIALEAGEIVLSGAITAAVDAAAGDVFTVS 245 Query 250 FTGLGLVRLSF 260 F GLG + L F Sbjct 246 FYGLGTLNLRF 256 >gi|284041910|ref|YP_003392250.1| 2-oxopent-4-enoate hydratase [Conexibacter woesei DSM 14684] gi|283946131|gb|ADB48875.1| 2-oxopent-4-enoate hydratase [Conexibacter woesei DSM 14684] Length=263 Score = 238 bits (608), Expect = 6e-61, Method: Compositional matrix adjust. Identities = 122/242 (51%), Positives = 161/242 (67%), Gaps = 0/242 (0%) Query 20 SRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGHKVGLSSPIMQQMMGVDEPD 79 SR PI LT + + DAYE+QL+N+R++VA ARV GHKVGL+S MQQ +GV EPD Sbjct 19 SRTPIAPLTERWDGMTIADAYEVQLLNVRRQVAASARVRGHKVGLASKAMQQQVGVHEPD 78 Query 80 YGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGAGCTEDDVLAATEALVPAIE 139 +GHLL DM +E+ + A R+++PRVEVE F+L L G G T DV+ A + ++P++E Sbjct 79 FGHLLHDMFAYEEEALAADRFIAPRVEVEPAFVLGRSLAGPGVTVADVVRAVDCVLPSLE 138 Query 140 LIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVRAIDAKLTRNGEVVAEGRSD 199 ++D+R+ DW+I+I DT+ADNAS+ VLG + D D R + A L+ NGE+ G + Sbjct 139 IVDSRVADWRIRIEDTVADNASSGALVLGGRPLRLVDFDPRTVGASLSINGEIRQTGSTA 198 Query 200 AVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEARAGDEFVADFTGLGLVRLS 259 AVLGNP TAVAWLA KV +FGV L +GD+VLPGSCT GD ADF LG V Sbjct 199 AVLGNPLTAVAWLANKVATFGVELAEGDVVLPGSCTRMEPIAPGDVVRADFDRLGHVAFH 258 Query 260 FE 261 E Sbjct 259 VE 260 >gi|333924103|ref|YP_004497683.1| 2-oxopent-4-enoate hydratase [Desulfotomaculum carboxydivorans CO-1-SRB] gi|333749664|gb|AEF94771.1| 2-oxopent-4-enoate hydratase [Desulfotomaculum carboxydivorans CO-1-SRB] Length=257 Score = 238 bits (607), Expect = 7e-61, Method: Compositional matrix adjust. Identities = 117/251 (47%), Positives = 169/251 (68%), Gaps = 0/251 (0%) Query 10 LAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGHKVGLSSPIM 69 +AA+L +AE ++ PI LT +P+++V DAY+IQL + ++ G +VVG K+GL+S M Sbjct 6 IAAELLRAEATQTPIDTLTDTYPDLNVEDAYQIQLAQVDMQLKTGHKVVGKKIGLTSKGM 65 Query 70 QQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGAGCTEDDVLA 129 QQ++GV+EPDYGHL + M + E P + + + PR+E E+ F+L L G G DV Sbjct 66 QQLLGVNEPDYGHLFEHMLLMEGEPCRRDQLIWPRIEGELAFVLKDRLKGPGVNIADVYR 125 Query 130 ATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVRAIDAKLTRN 189 AT ++PAIE++D+RI+DW+IK+ DT+ADN S+A FVLG+ VP +LD+R I L +N Sbjct 126 ATAGIMPAIEIVDSRIRDWKIKLPDTVADNGSSARFVLGSRMVPVEELDLRHIGMVLEKN 185 Query 190 GEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEARAGDEFVAD 249 GE+V G AV G+PA AVAWLA K+ ++ + L G+I+L G+ T AV+A AGD F Sbjct 186 GEMVNNGAGAAVWGHPAAAVAWLANKLAAYDIALEPGEIILSGAITAAVDANAGDVFTVS 245 Query 250 FTGLGLVRLSF 260 F LG + L F Sbjct 246 FHSLGTLNLKF 256 >gi|323702337|ref|ZP_08114002.1| 2-oxopent-4-enoate hydratase [Desulfotomaculum nigrificans DSM 574] gi|323532643|gb|EGB22517.1| 2-oxopent-4-enoate hydratase [Desulfotomaculum nigrificans DSM 574] Length=257 Score = 238 bits (606), Expect = 9e-61, Method: Compositional matrix adjust. Identities = 117/251 (47%), Positives = 169/251 (68%), Gaps = 0/251 (0%) Query 10 LAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGHKVGLSSPIM 69 +AA+L +AE ++ PI LT +P+++V DAY+IQL + ++ G +VVG K+GL+S M Sbjct 6 IAAELLRAEATQTPIDTLTDTYPDLNVEDAYQIQLAQVDMQLKMGHKVVGKKIGLTSKGM 65 Query 70 QQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGAGCTEDDVLA 129 QQ++GV+EPDYGHL + M + E P + + + PR+E E+ F+L L G G DV Sbjct 66 QQLLGVNEPDYGHLFEHMLLMEGEPCRRDQLIWPRIEGELAFVLKDRLKGPGVNIADVYR 125 Query 130 ATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVRAIDAKLTRN 189 AT ++PAIE++D+RI+DW+IK+ DT+ADN S+A FVLG+ VP +LD+R I L +N Sbjct 126 ATAGIMPAIEIVDSRIRDWKIKLPDTVADNGSSARFVLGSRMVPVEELDLRHIGMVLEKN 185 Query 190 GEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEARAGDEFVAD 249 GE+V G AV G+PA AVAWLA K+ ++ + L G+I+L G+ T AV+A AGD F Sbjct 186 GEMVNNGAGAAVWGHPAAAVAWLANKLAAYDIALEPGEIILSGAITAAVDANAGDVFTVS 245 Query 250 FTGLGLVRLSF 260 F LG + L F Sbjct 246 FHSLGTLNLKF 256 >gi|78042951|ref|YP_360112.1| hydratase/decarboxylase family protein [Carboxydothermus hydrogenoformans Z-2901] gi|77995066|gb|ABB13965.1| hydratase/decarboxylase family protein [Carboxydothermus hydrogenoformans Z-2901] Length=262 Score = 235 bits (600), Expect = 4e-60, Method: Compositional matrix adjust. Identities = 122/259 (48%), Positives = 171/259 (67%), Gaps = 2/259 (0%) Query 4 DATRDELAAD-LAQAERSRDPIGQ-LTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGHK 61 D T ++AA+ L +A + R + Q LT +P+I + DAY+IQLIN+ ++ A G +VVG K Sbjct 2 DKTALQVAAEALWKACQERKALEQPLTEIYPDITITDAYQIQLINVDKKKALGRKVVGKK 61 Query 62 VGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGAG 121 +GL+S MQQM+GV EPDYGH+ DDM E+ + S+ L P++E E+ F+L L G G Sbjct 62 IGLTSRAMQQMLGVHEPDYGHVTDDMIGDEEVAISRSKLLQPKIEAEIAFVLKDRLVGPG 121 Query 122 CTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVRA 181 T VL AT ++P E+ID+RIKDW+IKI DTIADNAS+A VLG+ +P ++++ Sbjct 122 VTLTKVLQATAGVIPVFEIIDSRIKDWKIKIQDTIADNASSAMVVLGSKLIPVDQVNLKY 181 Query 182 IDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEAR 241 + L +NGE++ AVLG+PA AVAWLA K+ FG+ L G+I+L GS T A E Sbjct 182 VGLVLEKNGEIIDTAAGAAVLGHPALAVAWLANKLSEFGIALEPGEIILSGSLTKAYEVT 241 Query 242 AGDEFVADFTGLGLVRLSF 260 D FVA+F LG V++ F Sbjct 242 GNDHFVANFGPLGSVKVKF 260 >gi|297530341|ref|YP_003671616.1| 2-oxopent-4-enoate hydratase [Geobacillus sp. C56-T3] gi|297253593|gb|ADI27039.1| 2-oxopent-4-enoate hydratase [Geobacillus sp. C56-T3] Length=259 Score = 234 bits (598), Expect = 6e-60, Method: Compositional matrix adjust. Identities = 122/253 (49%), Positives = 162/253 (65%), Gaps = 0/253 (0%) Query 8 DELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGHKVGLSSP 67 D+ A L AER R P+ LT + V DAY+IQL I+Q+V EG R+VG K+GL+S Sbjct 5 DDCAELLLAAERERRPLSPLTTLDSALTVYDAYQIQLRTIKQKVKEGRRIVGKKIGLTSK 64 Query 68 IMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGAGCTEDDV 127 MQQ++GVD+PDYGHLLDDM V V R L P+VE E+ F+L DL G T DV Sbjct 65 AMQQLLGVDQPDYGHLLDDMAVENGGVVSWERVLQPKVEAEIAFVLKRDLVGPRVTVIDV 124 Query 128 LAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVRAIDAKLT 187 L AT+ +VPA+E++D+RI DW I++ DTIADNAS+ +VLG R+ +D+ I L Sbjct 125 LLATDYIVPALEIVDSRIADWNIRLEDTIADNASSGLYVLGEKRLSVHAVDIAQIGMALY 184 Query 188 RNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEARAGDEFV 247 RNG++ G A LG+PA VAWLA K+ +G L+ G+++L G+ + AV A GD F Sbjct 185 RNGKLANTGVGAAALGHPAFCVAWLANKLYEYGKELKAGEVILSGALSAAVSAEPGDVFF 244 Query 248 ADFTGLGLVRLSF 260 A F LG VR+ F Sbjct 245 ARFAHLGEVRVEF 257 >gi|261419701|ref|YP_003253383.1| 2-oxopent-4-enoate hydratase [Geobacillus sp. Y412MC61] gi|319766519|ref|YP_004132020.1| 2-oxopent-4-enoate hydratase [Geobacillus sp. Y412MC52] gi|261376158|gb|ACX78901.1| 2-oxopent-4-enoate hydratase [Geobacillus sp. Y412MC61] gi|317111385|gb|ADU93877.1| 2-oxopent-4-enoate hydratase [Geobacillus sp. Y412MC52] Length=259 Score = 233 bits (595), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 121/253 (48%), Positives = 161/253 (64%), Gaps = 0/253 (0%) Query 8 DELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGHKVGLSSP 67 D+ A L AER R P+ LT + V DAY+IQL I+Q+V EG R+VG K+GL+S Sbjct 5 DDCAELLLAAERERRPLSPLTTLDSALTVYDAYQIQLRTIKQKVKEGRRIVGKKIGLTSK 64 Query 68 IMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGAGCTEDDV 127 MQQ++GVD+PDYGHLLDDM V V R L P+VE E+ F+L DL G T DV Sbjct 65 AMQQLLGVDQPDYGHLLDDMAVENGGVVSWERVLQPKVEAEIAFVLKRDLVGPRVTVIDV 124 Query 128 LAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVRAIDAKLT 187 L AT+ +VPA+E++D+RI DW I++ DTIADNAS+ +VLG R+ +D+ I L Sbjct 125 LLATDYIVPALEIVDSRIADWNIRLEDTIADNASSGLYVLGEKRLSVHAVDIAQIGMALY 184 Query 188 RNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEARAGDEFV 247 RNG++ G LG+PA VAWLA K+ +G L+ G+++L G+ + AV A GD F Sbjct 185 RNGKLANTGVGATALGHPAFCVAWLANKLYEYGKELKAGEVILSGALSAAVSAEPGDVFF 244 Query 248 ADFTGLGLVRLSF 260 A F LG VR+ F Sbjct 245 ARFAHLGEVRVEF 257 >gi|78044351|ref|YP_360156.1| hydratase/decarboxylase family protein [Carboxydothermus hydrogenoformans Z-2901] gi|77996466|gb|ABB15365.1| hydratase/decarboxylase family protein [Carboxydothermus hydrogenoformans Z-2901] Length=262 Score = 232 bits (591), Expect = 5e-59, Method: Compositional matrix adjust. Identities = 118/248 (48%), Positives = 164/248 (67%), Gaps = 1/248 (0%) Query 14 LAQAERSRDPIGQ-LTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGHKVGLSSPIMQQM 72 L +A + R + Q LT +P+I + DAY+IQLIN+ ++ A G +VVG K+GL+S MQQM Sbjct 13 LWKACQERKALEQPLTEIYPDITIGDAYQIQLINVDKKKALGRKVVGKKIGLTSRAMQQM 72 Query 73 MGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGAGCTEDDVLAATE 132 +GV EPDYGH+ DDM E+ + S+ L P++E E+ F+L L G G T VL AT Sbjct 73 LGVHEPDYGHVTDDMIGDEEVAISRSKLLQPKIEAEIAFVLKDRLVGPGVTLTKVLQATA 132 Query 133 ALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVRAIDAKLTRNGEV 192 ++P E+ID+RIKDW+IKI DTIADNAS+A VLG+ +P ++++ + L +NGE+ Sbjct 133 GVIPVFEIIDSRIKDWKIKIQDTIADNASSAMVVLGSKLIPVDQVNLKYVGLVLEKNGEI 192 Query 193 VAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEARAGDEFVADFTG 252 + AVLG+PA AVAWLA K+ FG+ L G+I+L GS T A E D FVA+F Sbjct 193 IDTAAGAAVLGHPALAVAWLANKLSEFGIALEPGEIILSGSLTKAYEVTGNDHFVANFGP 252 Query 253 LGLVRLSF 260 LG V++ F Sbjct 253 LGSVKVKF 260 >gi|167784118|gb|AAZ76889.2| 2-hydroxypenta-2,4-dienoate-hydratase [Geobacillus stearothermophilus] Length=256 Score = 231 bits (590), Expect = 6e-59, Method: Compositional matrix adjust. Identities = 116/252 (47%), Positives = 167/252 (67%), Gaps = 0/252 (0%) Query 9 ELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGHKVGLSSPI 68 + A L +AE++R + LTA P I V AY++QL+NI++++ G R+VG K+GL+S Sbjct 3 QWANHLIEAEKNRKGVEPLTALDPGITVAAAYQVQLVNIQRKMDAGQRIVGKKIGLTSLA 62 Query 69 MQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGAGCTEDDVL 128 MQ+++GV+EPDYGHLLD+M V + + L P+VE E+ F+L DL G TE DVL Sbjct 63 MQKLLGVNEPDYGHLLDEMMVENGRKLPYEKVLQPKVEAEIAFVLKKDLTGPRVTELDVL 122 Query 129 AATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVRAIDAKLTR 188 AT+ +VPA+E++D+RI W IK+ DTIADNAS+ FVLG +V LD+ I L + Sbjct 123 QATDYIVPALEIVDSRIAGWNIKLQDTIADNASSGFFVLGEKQVAIDSLDLSRIGMVLYK 182 Query 189 NGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEARAGDEFVA 248 NG+++ G A LGNPAT VAWLA K+ +G+ L+ G+++L G+ + A++A GD F A Sbjct 183 NGDIMNTGVGAAALGNPATCVAWLANKLFEYGMALKAGEVILSGALSAAIDAEPGDYFQA 242 Query 249 DFTGLGLVRLSF 260 F LG V + F Sbjct 243 RFAHLGEVSVRF 254 >gi|302547426|ref|ZP_07299768.1| 2-oxopent-4-enoate hydratase [Streptomyces hygroscopicus ATCC 53653] gi|302465044|gb|EFL28137.1| 2-oxopent-4-enoate hydratase [Streptomyces himastatinicus ATCC 53653] Length=260 Score = 231 bits (590), Expect = 7e-59, Method: Compositional matrix adjust. Identities = 129/259 (50%), Positives = 167/259 (65%), Gaps = 0/259 (0%) Query 2 LRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGHK 61 + DA R E L +AER PI LT P ++V DAY +Q NI +R+A GA VVGHK Sbjct 1 MTDAQRAEAGRLLREAERDVTPIDPLTELMPGLEVADAYAVQRDNIARRLAAGATVVGHK 60 Query 62 VGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGAG 121 VGL++ MQQ++GVDEPD+GHLLDDM + V A+RY +PR+E E+ F LA L G G Sbjct 61 VGLTAAAMQQLLGVDEPDFGHLLDDMVHRDGGSVPAARYCAPRIEPEICFRLAEPLRGPG 120 Query 122 CTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVRA 181 T +DVLAATEA+ PA+E++D+RI+DW+I + DT+ADNAS+AG V GA D+ A Sbjct 121 VTVEDVLAATEAVAPALEIVDSRIRDWKITLADTVADNASSAGLVWGAWTPLVEAPDLAA 180 Query 182 IDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEAR 241 + A L +GE VA G VLG+PA AVAWL + FG L G +VLPG+ T A Sbjct 181 VVADLLVDGERVASGSGSEVLGHPAAAVAWLVNTLAEFGTALEPGHLVLPGTMTGAPFVS 240 Query 242 AGDEFVADFTGLGLVRLSF 260 AG A F+ LG V ++F Sbjct 241 AGQRIEARFSDLGPVSVTF 259 >gi|121534036|ref|ZP_01665862.1| 4-oxalocrotonate decarboxylase [Thermosinus carboxydivorans Nor1] gi|121307547|gb|EAX48463.1| 4-oxalocrotonate decarboxylase [Thermosinus carboxydivorans Nor1] Length=260 Score = 231 bits (588), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 119/253 (48%), Positives = 161/253 (64%), Gaps = 0/253 (0%) Query 8 DELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGHKVGLSSP 67 ++LA +L QAE +R PI LTA +P+I DAY++QL+ + R ++G VVG K+GL+S Sbjct 7 EQLAKELYQAETTRTPIEALTARYPDITNEDAYQVQLVGQKLRESDGHVVVGRKIGLTSK 66 Query 68 IMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGAGCTEDDV 127 MQ +GV EPDYG++ D M E V + ++P+VE E+ F+L DL G G T V Sbjct 67 AMQAALGVFEPDYGYITDRMMALEGDAVSMNELIAPKVEAEIAFVLKRDLAGPGVTVAQV 126 Query 128 LAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVRAIDAKLT 187 L AT ++PA+E+IDTRIKDW+IKI DTIAD AS +L +P LD+R + L Sbjct 127 LEATAGIMPALEIIDTRIKDWKIKIQDTIADGASIGKVILSGKLLPVGALDMRYMGMVLE 186 Query 188 RNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEARAGDEFV 247 +NGE+VA G AVLG+PA AVAWLA K+ +G+ L+ GDI++ GS T A AGD Sbjct 187 KNGEIVATGAGAAVLGHPANAVAWLANKLSQYGIILKAGDIIMSGSLTAACPVAAGDNIR 246 Query 248 ADFTGLGLVRLSF 260 A F +G V F Sbjct 247 ATFDHIGSVSARF 259 >gi|239937272|dbj|BAH79101.1| 2-hydroxypenta-2,4-dienoate hydratase [Paenibacillus sp. JJ-1b] Length=260 Score = 229 bits (584), Expect = 3e-58, Method: Compositional matrix adjust. Identities = 116/253 (46%), Positives = 165/253 (66%), Gaps = 0/253 (0%) Query 9 ELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGHKVGLSSPI 68 + A L QA + LTA E+ V DAY++QLINI ++V G+ +VG K+GL+S Sbjct 7 KYAEHLEQATLQGKGVEPLTAIDAELTVHDAYQVQLINIDKKVKSGSVIVGKKIGLTSLA 66 Query 69 MQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGAGCTEDDVL 128 MQQ++ VD+PDYGHLLDDM V + SR L PRVE E+ F+L DL G + DVL Sbjct 67 MQQLLKVDQPDYGHLLDDMVVENGGVIPFSRMLQPRVEAEIAFVLKQDLIGPNVSALDVL 126 Query 129 AATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVRAIDAKLTR 188 AT+ ++PA+E++D+R+ DW+IK+ DTIADNAS+ +VLG V P +D+ + + L + Sbjct 127 LATDYVLPALEIVDSRVADWKIKLQDTIADNASSGLYVLGGKPVKPDAIDLPLVGSVLYK 186 Query 189 NGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEARAGDEFVA 248 NGE++ G A LG+PA VAWLA K+ FG+ L+ G+++L G+ + AV A+ GD F A Sbjct 187 NGEIMNTGVGAAALGDPAVCVAWLANKLFEFGISLKAGEVILSGALSAAVHAKPGDHFTA 246 Query 249 DFTGLGLVRLSFE 261 LG V +SF+ Sbjct 247 RLAHLGEVSVSFQ 259 >gi|258654208|ref|YP_003203364.1| 2-oxopent-4-enoate hydratase [Nakamurella multipartita DSM 44233] gi|258557433|gb|ACV80375.1| 2-oxopent-4-enoate hydratase [Nakamurella multipartita DSM 44233] Length=267 Score = 229 bits (583), Expect = 4e-58, Method: Compositional matrix adjust. Identities = 117/261 (45%), Positives = 168/261 (65%), Gaps = 0/261 (0%) Query 1 MLRDATRDELAADLAQAERSRDPIGQLTAAHPEIDVVDAYEIQLINIRQRVAEGARVVGH 60 M D+ +LA +L +A+R+ PI LT +P++ V DAY IQ INI +RVA G +++G Sbjct 1 MTADSLHQQLADELWEADRTAKPISPLTERYPDLVVEDAYAIQTINIDRRVAAGEKIIGR 60 Query 61 KVGLSSPIMQQMMGVDEPDYGHLLDDMQVFEDTPVQASRYLSPRVEVEVGFILAADLPGA 120 KVGL+S MQQ++GV+EPD+G L D M + + P+ + + PRVE E+ F++ DL G Sbjct 61 KVGLTSAPMQQLLGVNEPDFGVLTDAMFIEDGDPIDLNDLVQPRVEAELAFVMETDLAGP 120 Query 121 GCTEDDVLAATEALVPAIELIDTRIKDWQIKICDTIADNASAAGFVLGAARVPPADLDVR 180 G T L A +PA+E++D+R+ DW+IK+ DT++DNAS+ V+G +P DLD+R Sbjct 121 GVTTAGALRAIAGALPAVEIVDSRVADWKIKLVDTVSDNASSGLLVVGGRMLPVNDLDLR 180 Query 181 AIDAKLTRNGEVVAEGRSDAVLGNPATAVAWLAGKVESFGVRLRKGDIVLPGSCTFAVEA 240 + ++R+GEV+ G A LGNPA VAWLA K+ S G L+ GDIVLPG+ V Sbjct 181 TLGVVVSRHGEVIDTGAGAAALGNPARCVAWLANKLGSLGSGLKAGDIVLPGAVHKMVPV 240 Query 241 RAGDEFVADFTGLGLVRLSFE 261 ++GD F ADF LG V + F Sbjct 241 QSGDVFRADFAHLGPVTVRFS 261 Lambda K H 0.319 0.136 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Effective search space used: 388543189928 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects Posted date: Sep 5, 2011 4:36 AM Number of letters in database: 5,219,829,388 Number of sequences in database: 15,229,318 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 11 Window for multiple hits: 40