BLASTP 2.2.25+


Reference:
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database
search programs", Nucleic Acids Res. 25:3389-3402.



Reference for composition-based statistics:
Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.



Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
           15,229,318 sequences; 5,219,829,388 total letters



Query= Rv3723

Length=254
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

gi|15610859|ref|NP_218240.1|  transmembrane protein [Mycobacteriu...   502    2e-140
gi|340628697|ref|YP_004747149.1|  hypothetical protein MCAN_37451...   502    2e-140
gi|298527200|ref|ZP_07014609.1|  conserved hypothetical protein [...   499    1e-139
gi|15828260|ref|NP_302523.1|  hypothetical protein ML2337 [Mycoba...   297    8e-79 
gi|118619470|ref|YP_907802.1|  hypothetical protein MUL_4318 [Myc...   255    3e-66 
gi|183985211|ref|YP_001853502.1|  hypothetical protein MMAR_5242 ...   255    4e-66 
gi|240171033|ref|ZP_04749692.1|  putative transmembrane protein [...   253    2e-65 
gi|145221871|ref|YP_001132549.1|  hypothetical protein Mflv_1279 ...   251    7e-65 
gi|296166817|ref|ZP_06849235.1|  conserved hypothetical protein [...   248    4e-64 
gi|254818501|ref|ZP_05223502.1|  hypothetical protein MintA_01184...   244    1e-62 
gi|120406479|ref|YP_956308.1|  hypothetical protein Mvan_5536 [My...   234    6e-60 
gi|342859927|ref|ZP_08716579.1|  hypothetical protein MCOL_13645 ...   234    7e-60 
gi|254773384|ref|ZP_05214900.1|  hypothetical protein MaviaA2_017...   224    9e-57 
gi|336460785|gb|EGO39671.1|  hypothetical protein MAPs_37260 [Myc...   224    1e-56 
gi|41406422|ref|NP_959258.1|  hypothetical protein MAP0324 [Mycob...   224    1e-56 
gi|118470427|ref|YP_890506.1|  hypothetical protein MSMEG_6288 [M...   214    8e-54 
gi|126437842|ref|YP_001073533.1|  hypothetical protein Mjls_5279 ...   202    5e-50 
gi|118465404|ref|YP_879665.1|  hypothetical protein MAV_0378 [Myc...   202    5e-50 
gi|108801874|ref|YP_642071.1|  hypothetical protein Mmcs_4911 [My...   200    2e-49 
gi|333992653|ref|YP_004525267.1|  hypothetical protein JDM601_401...   160    1e-37 
gi|169627388|ref|YP_001701037.1|  hypothetical protein MAB_0283c ...   152    4e-35 
gi|341878512|gb|EGT34447.1|  CBN-TAG-279 protein [Caenorhabditis ...  47.8    0.002 
gi|255284218|ref|ZP_05348773.1|  glycogen synthase [Bryantella fo...  37.7    1.5   


>gi|15610859|ref|NP_218240.1| transmembrane protein [Mycobacterium tuberculosis H37Rv]
 gi|15843344|ref|NP_338381.1| hypothetical protein MT3826 [Mycobacterium tuberculosis CDC1551]
 gi|31794895|ref|NP_857388.1| transmembrane protein [Mycobacterium bovis AF2122/97]
 76 more sequence titles
 Length=254

 Score =  502 bits (1293),  Expect = 2e-140, Method: Compositional matrix adjust.
 Identities = 254/254 (100%), Positives = 254/254 (100%), Gaps = 0/254 (0%)

Query  1    MGRKVAVLWHASFSIGAGVLYFYFVLPRWPELMGDTGHSLGTGLRIATGALVGLAALPVV  60
            MGRKVAVLWHASFSIGAGVLYFYFVLPRWPELMGDTGHSLGTGLRIATGALVGLAALPVV
Sbjct  1    MGRKVAVLWHASFSIGAGVLYFYFVLPRWPELMGDTGHSLGTGLRIATGALVGLAALPVV  60

Query  61   FTLLRTRKPELGTPQLALSMRIWSIMAHVLAGALIVGTAISEVWLSLDAAGQWLFGIYGA  120
            FTLLRTRKPELGTPQLALSMRIWSIMAHVLAGALIVGTAISEVWLSLDAAGQWLFGIYGA
Sbjct  61   FTLLRTRKPELGTPQLALSMRIWSIMAHVLAGALIVGTAISEVWLSLDAAGQWLFGIYGA  120

Query  121  AAAIAVLGFFGFYLSFVAELPPPPPKPLKPKKPKQRRLRRKKTAKGDEAEPEAAEEAENT  180
            AAAIAVLGFFGFYLSFVAELPPPPPKPLKPKKPKQRRLRRKKTAKGDEAEPEAAEEAENT
Sbjct  121  AAAIAVLGFFGFYLSFVAELPPPPPKPLKPKKPKQRRLRRKKTAKGDEAEPEAAEEAENT  180

Query  181  ELAAQEDEEAVEAPPESIESPGGEPESATREAPAAETATAEEPRGGLRNRRPTGKTSHRR  240
            ELAAQEDEEAVEAPPESIESPGGEPESATREAPAAETATAEEPRGGLRNRRPTGKTSHRR
Sbjct  181  ELAAQEDEEAVEAPPESIESPGGEPESATREAPAAETATAEEPRGGLRNRRPTGKTSHRR  240

Query  241  RRTRSGVQVAKVDE  254
            RRTRSGVQVAKVDE
Sbjct  241  RRTRSGVQVAKVDE  254


>gi|340628697|ref|YP_004747149.1| hypothetical protein MCAN_37451 [Mycobacterium canettii CIPT 
140010059]
 gi|340006887|emb|CCC46076.1| putative conserved transmembrane protein [Mycobacterium canettii 
CIPT 140010059]
Length=254

 Score =  502 bits (1292),  Expect = 2e-140, Method: Compositional matrix adjust.
 Identities = 253/254 (99%), Positives = 254/254 (100%), Gaps = 0/254 (0%)

Query  1    MGRKVAVLWHASFSIGAGVLYFYFVLPRWPELMGDTGHSLGTGLRIATGALVGLAALPVV  60
            MGRKVAVLWHASFSIGAGVLYFYFVLPRWPELMGDTGHSLGTGLRIATGAL+GLAALPVV
Sbjct  1    MGRKVAVLWHASFSIGAGVLYFYFVLPRWPELMGDTGHSLGTGLRIATGALIGLAALPVV  60

Query  61   FTLLRTRKPELGTPQLALSMRIWSIMAHVLAGALIVGTAISEVWLSLDAAGQWLFGIYGA  120
            FTLLRTRKPELGTPQLALSMRIWSIMAHVLAGALIVGTAISEVWLSLDAAGQWLFGIYGA
Sbjct  61   FTLLRTRKPELGTPQLALSMRIWSIMAHVLAGALIVGTAISEVWLSLDAAGQWLFGIYGA  120

Query  121  AAAIAVLGFFGFYLSFVAELPPPPPKPLKPKKPKQRRLRRKKTAKGDEAEPEAAEEAENT  180
            AAAIAVLGFFGFYLSFVAELPPPPPKPLKPKKPKQRRLRRKKTAKGDEAEPEAAEEAENT
Sbjct  121  AAAIAVLGFFGFYLSFVAELPPPPPKPLKPKKPKQRRLRRKKTAKGDEAEPEAAEEAENT  180

Query  181  ELAAQEDEEAVEAPPESIESPGGEPESATREAPAAETATAEEPRGGLRNRRPTGKTSHRR  240
            ELAAQEDEEAVEAPPESIESPGGEPESATREAPAAETATAEEPRGGLRNRRPTGKTSHRR
Sbjct  181  ELAAQEDEEAVEAPPESIESPGGEPESATREAPAAETATAEEPRGGLRNRRPTGKTSHRR  240

Query  241  RRTRSGVQVAKVDE  254
            RRTRSGVQVAKVDE
Sbjct  241  RRTRSGVQVAKVDE  254


>gi|298527200|ref|ZP_07014609.1| conserved hypothetical protein [Mycobacterium tuberculosis 94_M4241A]
 gi|298496994|gb|EFI32288.1| conserved hypothetical protein [Mycobacterium tuberculosis 94_M4241A]
Length=254

 Score =  499 bits (1285),  Expect = 1e-139, Method: Compositional matrix adjust.
 Identities = 253/254 (99%), Positives = 253/254 (99%), Gaps = 0/254 (0%)

Query  1    MGRKVAVLWHASFSIGAGVLYFYFVLPRWPELMGDTGHSLGTGLRIATGALVGLAALPVV  60
            MGRKVAVLWHASFSIGAGVLYFYFVLPRWPELMGDTGHSLGTGLRIATGALVGLAALPVV
Sbjct  1    MGRKVAVLWHASFSIGAGVLYFYFVLPRWPELMGDTGHSLGTGLRIATGALVGLAALPVV  60

Query  61   FTLLRTRKPELGTPQLALSMRIWSIMAHVLAGALIVGTAISEVWLSLDAAGQWLFGIYGA  120
            FTLLRTRKPELGTPQLALSMRIWSIMAHVLAGALIVGTAISEVWLSLDAAGQWLFGIYGA
Sbjct  61   FTLLRTRKPELGTPQLALSMRIWSIMAHVLAGALIVGTAISEVWLSLDAAGQWLFGIYGA  120

Query  121  AAAIAVLGFFGFYLSFVAELPPPPPKPLKPKKPKQRRLRRKKTAKGDEAEPEAAEEAENT  180
            AAAIAVLGFFGFYLSFVAELP PPPKPLKPKKPKQRRLRRKKTAKGDEAEPEAAEEAENT
Sbjct  121  AAAIAVLGFFGFYLSFVAELPAPPPKPLKPKKPKQRRLRRKKTAKGDEAEPEAAEEAENT  180

Query  181  ELAAQEDEEAVEAPPESIESPGGEPESATREAPAAETATAEEPRGGLRNRRPTGKTSHRR  240
            ELAAQEDEEAVEAPPESIESPGGEPESATREAPAAETATAEEPRGGLRNRRPTGKTSHRR
Sbjct  181  ELAAQEDEEAVEAPPESIESPGGEPESATREAPAAETATAEEPRGGLRNRRPTGKTSHRR  240

Query  241  RRTRSGVQVAKVDE  254
            RRTRSGVQVAKVDE
Sbjct  241  RRTRSGVQVAKVDE  254


>gi|15828260|ref|NP_302523.1| hypothetical protein ML2337 [Mycobacterium leprae TN]
 gi|221230737|ref|YP_002504153.1| hypothetical protein MLBr_02337 [Mycobacterium leprae Br4923]
 gi|3150101|emb|CAA19153.1| hypothetical protein MLCB2407.13c [Mycobacterium leprae]
 gi|13093953|emb|CAC31853.1| putative membrane protein [Mycobacterium leprae]
 gi|219933844|emb|CAR72435.1| putative membrane protein [Mycobacterium leprae Br4923]
Length=250

 Score =  297 bits (761),  Expect = 8e-79, Method: Compositional matrix adjust.
 Identities = 163/253 (65%), Positives = 186/253 (74%), Gaps = 9/253 (3%)

Query  1    MGRKVAVLWHASFSIGAGVLYFYFVLPRWPELMGDTGHSLGTGLRIATGALVGLAALPVV  60
            MG KVA LWHASFSIGAGVLYFYFVLPRWPEL+G+T H+LGT LRI TG L GLAALPVV
Sbjct  1    MGYKVATLWHASFSIGAGVLYFYFVLPRWPELIGETTHTLGTTLRIVTGVLFGLAALPVV  60

Query  61   FTLLRTRKPELGTPQLALSMRIWSIMAHVLAGALIVGTAISEVWLSLDAAGQWLFGIYGA  120
            FTLLR++  ELG PQLALS+R WSI+AHVL+G LIV TAI E+WLS D A Q LFGIYGA
Sbjct  61   FTLLRSQTSELGIPQLALSIRTWSIVAHVLSGVLIVSTAIGEIWLSSDTAVQCLFGIYGA  120

Query  121  AAAIAVLGFFGFYLSFVAELPPPPPKPLKPKKPKQRRLRRKKTAKGDEAEPEAAEEAENT  180
            AAA AVLGF  FYLSFVA LPPP PKP+K KK  QRR+RR+K  K +EAE E A+  E+ 
Sbjct  121  AAASAVLGFVAFYLSFVAALPPPSPKPVKLKKANQRRIRRRKGRKDNEAEDEEADAGEHN  180

Query  181  ELAAQEDEEAVEAPPESIESPGGEPESATREAP----AAETATAEEPRGGLRNRRPTGKT  236
            E       E V +     E+P   PES  ++ P    A +T   +EPR GL NRRPTGK 
Sbjct  181  EAETPAQAEEVTS-----ENPQPAPESGAQKEPDELLANKTEETDEPRRGLHNRRPTGKV  235

Query  237  SHRRRRTRSGVQV  249
            SH+RRR RSG+ V
Sbjct  236  SHQRRRARSGIAV  248


>gi|118619470|ref|YP_907802.1| hypothetical protein MUL_4318 [Mycobacterium ulcerans Agy99]
 gi|118571580|gb|ABL06331.1| conserved membrane protein [Mycobacterium ulcerans Agy99]
Length=271

 Score =  255 bits (652),  Expect = 3e-66, Method: Compositional matrix adjust.
 Identities = 143/170 (85%), Positives = 156/170 (92%), Gaps = 1/170 (0%)

Query  1    MGRKVAVLWHASFSIGAGVLYFYFVLPRWPELMGDTGHSLGTGLRIATGALVGLAALPVV  60
            MGRKVA+LWHASFSIGAGVLYF+FV+PRWPELMGDT HSLGTGLRI  GAL GLAALPVV
Sbjct  1    MGRKVAMLWHASFSIGAGVLYFFFVVPRWPELMGDTAHSLGTGLRITAGALFGLAALPVV  60

Query  61   FTLLRTRKPELGTPQLALSMRIWSIMAHVLAGALIVGTAISEVWLSLDAAGQWLFGIYGA  120
            FTLLRTRKPELGTP LALS+R WSI+AHVLAG LI+GTAISE+WLSLD+ GQWLFGIYGA
Sbjct  61   FTLLRTRKPELGTPALALSIRTWSIVAHVLAGVLIIGTAISEIWLSLDSVGQWLFGIYGA  120

Query  121  AAAIAVLGFFGFYLSFVAELPPPPPKPLKPKKPKQRRLRRKKTAKGDEAE  170
            AAAIAVLGFF FYLSFVAELPPPPPKP+K K+PKQRR RRKK  +GD+ +
Sbjct  121  AAAIAVLGFFAFYLSFVAELPPPPPKPIKVKEPKQRR-RRKKGGEGDDVD  169


>gi|183985211|ref|YP_001853502.1| hypothetical protein MMAR_5242 [Mycobacterium marinum M]
 gi|183178537|gb|ACC43647.1| conserved membrane protein [Mycobacterium marinum M]
Length=280

 Score =  255 bits (652),  Expect = 4e-66, Method: Compositional matrix adjust.
 Identities = 144/169 (86%), Positives = 155/169 (92%), Gaps = 1/169 (0%)

Query  1    MGRKVAVLWHASFSIGAGVLYFYFVLPRWPELMGDTGHSLGTGLRIATGALVGLAALPVV  60
            MGRKVA+LWHASFSIGAGVLYF+FVLPRWPELMGDT HSLGTGLRI  GAL GLAALPVV
Sbjct  1    MGRKVAMLWHASFSIGAGVLYFFFVLPRWPELMGDTAHSLGTGLRITAGALFGLAALPVV  60

Query  61   FTLLRTRKPELGTPQLALSMRIWSIMAHVLAGALIVGTAISEVWLSLDAAGQWLFGIYGA  120
            FTLLRTRKPELGTP LALS+R WSI+AHVLAG LI+GTAISE+WLSLD+ GQWLFGIYGA
Sbjct  61   FTLLRTRKPELGTPALALSIRTWSIVAHVLAGVLIIGTAISEIWLSLDSVGQWLFGIYGA  120

Query  121  AAAIAVLGFFGFYLSFVAELPPPPPKPLKPKKPKQRRLRRKKTAKGDEA  169
            AAAIAVLGFF FYLSFVAELPPPPPKP+K K+PKQRR RRKK  +GD+ 
Sbjct  121  AAAIAVLGFFAFYLSFVAELPPPPPKPIKVKEPKQRR-RRKKGDEGDDV  168


>gi|240171033|ref|ZP_04749692.1| putative transmembrane protein [Mycobacterium kansasii ATCC 12478]
Length=286

 Score =  253 bits (647),  Expect = 2e-65, Method: Compositional matrix adjust.
 Identities = 137/162 (85%), Positives = 148/162 (92%), Gaps = 0/162 (0%)

Query  1    MGRKVAVLWHASFSIGAGVLYFYFVLPRWPELMGDTGHSLGTGLRIATGALVGLAALPVV  60
            M RK AV+WHASFSI AG+LYFYFVLPRW ELMG+T H+LGT LRI  GAL+GLAALPVV
Sbjct  1    MRRKWAVVWHASFSIAAGLLYFYFVLPRWFELMGETSHALGTTLRIVAGALIGLAALPVV  60

Query  61   FTLLRTRKPELGTPQLALSMRIWSIMAHVLAGALIVGTAISEVWLSLDAAGQWLFGIYGA  120
            FTLLRTRKPELGTPQLALS+R WSI+AHVLAG LI+GTAISE+WLSLD+AGQWLFGIYGA
Sbjct  61   FTLLRTRKPELGTPQLALSIRTWSIVAHVLAGVLIIGTAISEIWLSLDSAGQWLFGIYGA  120

Query  121  AAAIAVLGFFGFYLSFVAELPPPPPKPLKPKKPKQRRLRRKK  162
            AAAI VLGFF FYLSFVAELPPPPPKPLKPK  KQRR+RRKK
Sbjct  121  AAAIGVLGFFAFYLSFVAELPPPPPKPLKPKATKQRRIRRKK  162


>gi|145221871|ref|YP_001132549.1| hypothetical protein Mflv_1279 [Mycobacterium gilvum PYR-GCK]
 gi|315446394|ref|YP_004079273.1| hypothetical protein Mspyr1_49040 [Mycobacterium sp. Spyr1]
 gi|145214357|gb|ABP43761.1| conserved hypothetical protein [Mycobacterium gilvum PYR-GCK]
 gi|315264697|gb|ADU01439.1| hypothetical protein Mspyr1_49040 [Mycobacterium sp. Spyr1]
Length=245

 Score =  251 bits (641),  Expect = 7e-65, Method: Compositional matrix adjust.
 Identities = 152/251 (61%), Positives = 177/251 (71%), Gaps = 10/251 (3%)

Query  1    MGRKVAVLWHASFSIGAGVLYFYFVLPRWPELMGDTGHSLGTGLRIATGALVGLAALPVV  60
            MGR  AV+WHASFSI AGVLYF+FVLPR PEL+GDT H+LGT LRI  GAL+GLAALPVV
Sbjct  1    MGRTAAVIWHASFSIAAGVLYFFFVLPRTPELLGDTSHTLGTALRIVAGALIGLAALPVV  60

Query  61   FTLLRTRKPELGTPQLALSMRIWSIMAHVLAGALIVGTAISEVWLSLDAAGQWLFGIYGA  120
            FTLLRTR+PE GTP+LAL++R  SI  HVLA ALIVGTAISE+WLS+D+AGQWLFGIYGA
Sbjct  61   FTLLRTRRPEFGTPKLALNLRTASIALHVLAAALIVGTAISEIWLSVDSAGQWLFGIYGA  120

Query  121  AAAIAVLGFFGFYLSFVAELPPPPPKPLKPKKPKQRRLRRKKTAKGDEAEPEAAEEAENT  180
            AAAI +LG F FYL+FVAELPPPPPKPLK  K      R  +  KGD +E  A  E    
Sbjct  121  AAAIGLLGIFAFYLAFVAELPPPPPKPLKATK------RTGRGRKGDGSEDSADVEGSEG  174

Query  181  ELAAQEDEEAVEAPPESIESPGGEPESATREAPAAETATAEEPRGGLRNRRPTGKTS--H  238
            E+   E  + V          G + E A   A  ++   A++  G LRNRRPTGK +   
Sbjct  175  EVLESERTDGVTDADVDTGENGVKAEDAP--AAVSDADDADDADGKLRNRRPTGKGTGLG  232

Query  239  RRRRTRSGVQV  249
            RR+RTR GV V
Sbjct  233  RRKRTRGGVAV  243


>gi|296166817|ref|ZP_06849235.1| conserved hypothetical protein [Mycobacterium parascrofulaceum 
ATCC BAA-614]
 gi|295897833|gb|EFG77421.1| conserved hypothetical protein [Mycobacterium parascrofulaceum 
ATCC BAA-614]
Length=273

 Score =  248 bits (634),  Expect = 4e-64, Method: Compositional matrix adjust.
 Identities = 159/254 (63%), Positives = 180/254 (71%), Gaps = 15/254 (5%)

Query  1    MGRKVAVLWHASFSIGAGVLYFYFVLPRWPELMGDTGHSLGTGLRIATGALVGLAALPVV  60
            MGRK+AV WH  FS  AG LYF+FVLPRWPELMGDT H+LGT LRIATGAL GLAALPVV
Sbjct  1    MGRKIAVPWHLLFSFAAGALYFFFVLPRWPELMGDTTHTLGTALRIATGALFGLAALPVV  60

Query  61   FTLLRTRKPELGTPQLALSMRIWSIMAHVLAGALIVGTAISEVWLSLDAAGQWLFGIYGA  120
            F L R RKPELGTPQLAL++R+ SI+ HVLAG LI+GTAISE+WLSLDAAGQ+LF IYGA
Sbjct  61   FNLSRVRKPELGTPQLALNLRVASIVCHVLAGVLIIGTAISEIWLSLDAAGQYLFAIYGA  120

Query  121  AAAIAVLGFFGFYLSFVAELPPPPPKPLKPKKPKQRRLRRKKTAKGDEAEPEAAEEAEN-  179
            AAA+AVLGFF FYLSFVAELPPPPPKP+K KKPK RRLRRKK A+ DEAE   A+E ++ 
Sbjct  121  AAAVAVLGFFAFYLSFVAELPPPPPKPIKEKKPKTRRLRRKKGAEADEAEDTEADEVDDA  180

Query  180  ----------TELAAQEDEEAVEAPPESIESPGGEPESATREAPAAETAT----AEEPRG  225
                       E   +  E A    PE++ +   E          AE        E  + 
Sbjct  181  EAAEATEAVEAEDTEEAAETAETEQPETVTAEAPEATEEPPAEETAEAGEETEHTEAAQA  240

Query  226  GLRNRRPTGKTSHR  239
             LRNRRPTGK SHR
Sbjct  241  RLRNRRPTGKGSHR  254


>gi|254818501|ref|ZP_05223502.1| hypothetical protein MintA_01184 [Mycobacterium intracellulare 
ATCC 13950]
Length=276

 Score =  244 bits (622),  Expect = 1e-62, Method: Compositional matrix adjust.
 Identities = 166/254 (66%), Positives = 192/254 (76%), Gaps = 18/254 (7%)

Query  1    MGRKVAVLWHASFSIGAGVLYFYFVLPRWPELMGDTGHSLGTGLRIATGALVGLAALPVV  60
            MGRKVA+LWHASF+I AG LYF+FVLPRWPELMGDT HSLGT LRI TGALV LAALPVV
Sbjct  1    MGRKVAILWHASFTIAAGFLYFFFVLPRWPELMGDTAHSLGTALRIVTGALVALAALPVV  60

Query  61   FTLLRTRKPELGTPQLALSMRIWSIMAHVLAGALIVGTAISEVWLSLDAAGQWLFGIYGA  120
            FTLLRTRKPELGTPQ+ALS+R+ SI+AHVLAG LIVGTAISE+WLSLD AG+WLFGIYGA
Sbjct  61   FTLLRTRKPELGTPQMALSIRVASIVAHVLAGVLIVGTAISEIWLSLDVAGRWLFGIYGA  120

Query  121  AAAIAVLGFFGFYLSFVAELPPPPPKPLKPKKPKQRR---LRRKKTAKGDEAEPEAAEEA  177
            AAAIA+LGFF FYLSFVAELPPPPPKP+K KKPK+ +   LRRKK  + DEA    A+EA
Sbjct  121  AAAIALLGFFAFYLSFVAELPPPPPKPIKAKKPKKAKKGGLRRKKGEEADEAGE--ADEA  178

Query  178  ENTELAAQEDEEAVEAPPESIE------------SPGGEPESATREAPAAE-TATAEEPR  224
            ++ + A + +E       E+ E                +  + T  AP A+ T  +EEPR
Sbjct  179  DSADEAGEAEEADAVTEAEASEPDETDAATDAAAEAAPDEPAETEVAPVADATEESEEPR  238

Query  225  GGLRNRRPTGKTSH  238
              L+NRRPTGK SH
Sbjct  239  TKLQNRRPTGKGSH  252


>gi|120406479|ref|YP_956308.1| hypothetical protein Mvan_5536 [Mycobacterium vanbaalenii PYR-1]
 gi|119959297|gb|ABM16302.1| conserved hypothetical protein [Mycobacterium vanbaalenii PYR-1]
Length=252

 Score =  234 bits (598),  Expect = 6e-60, Method: Compositional matrix adjust.
 Identities = 151/252 (60%), Positives = 181/252 (72%), Gaps = 5/252 (1%)

Query  1    MGRKVAVLWHASFSIGAGVLYFYFVLPRWPELMGDTGHSLGTGLRIATGALVGLAALPVV  60
            MGR VAV+WHASFSI AGVLYF+FVLPR PEL+GDT  +LGT LR+  GAL+GLAALPVV
Sbjct  1    MGRTVAVIWHASFSIAAGVLYFFFVLPRTPELVGDTSPTLGTVLRVVVGALIGLAALPVV  60

Query  61   FTLLRTRKPELGTPQLALSMRIWSIMAHVLAGALIVGTAISEVWLSLDAAGQWLFGIYGA  120
            FTLLRTR+PE GTPQ+AL++R  SI  HVL+G LIVG A++E+W+SLD+AGQWLFGIYGA
Sbjct  61   FTLLRTRRPEFGTPQMALNLRTTSIALHVLSGVLIVGAAVAEIWISLDSAGQWLFGIYGA  120

Query  121  AAAIAVLGFFGFYLSFVAELPPPPPKPLKPKKPKQRRLRRKKTAKGDEAEPEAAE-EAEN  179
            AAA+A+LG   FYL+FVAELPPPPPKPLK KK K RR R       + A+ E A+ E E 
Sbjct  121  AAAVALLGVLAFYLAFVAELPPPPPKPLKVKKRKSRRGRSDAAEDTETADAETADAEVEE  180

Query  180  TELAAQEDEEAVEAPPESIESPGGEPESATREAPAAETATAEEPRGGLRNRRPTGKTS--  237
            TE A  E E          ++   EP     + PA++  T E   G L+NRRPTGK +  
Sbjct  181  TETADAETESVTPDADADADADAEEPVPTAEDDPASD--TPESGDGKLQNRRPTGKGAVL  238

Query  238  HRRRRTRSGVQV  249
             RR+RTR GV V
Sbjct  239  GRRKRTRGGVAV  250


>gi|342859927|ref|ZP_08716579.1| hypothetical protein MCOL_13645 [Mycobacterium colombiense CECT 
3035]
 gi|342132305|gb|EGT85534.1| hypothetical protein MCOL_13645 [Mycobacterium colombiense CECT 
3035]
Length=276

 Score =  234 bits (598),  Expect = 7e-60, Method: Compositional matrix adjust.
 Identities = 135/162 (84%), Positives = 150/162 (93%), Gaps = 0/162 (0%)

Query  1    MGRKVAVLWHASFSIGAGVLYFYFVLPRWPELMGDTGHSLGTGLRIATGALVGLAALPVV  60
            MGRKVA+ WHASFSI  G LYF+FVLPRWPELMGDT H+LGT +RI TGALV LAALPVV
Sbjct  1    MGRKVAIPWHASFSIAVGFLYFFFVLPRWPELMGDTAHTLGTAMRIVTGALVALAALPVV  60

Query  61   FTLLRTRKPELGTPQLALSMRIWSIMAHVLAGALIVGTAISEVWLSLDAAGQWLFGIYGA  120
            FTLLR+RKPELGTPQLALS+R+WSI+AHVLAG LI+GTAISE+WLSLDAAG+WLFGIYGA
Sbjct  61   FTLLRSRKPELGTPQLALSIRVWSIVAHVLAGVLIIGTAISEIWLSLDAAGRWLFGIYGA  120

Query  121  AAAIAVLGFFGFYLSFVAELPPPPPKPLKPKKPKQRRLRRKK  162
            AAAIA+LG F FYLSFVAELPPPPPKP+K KKPK+RR+RRKK
Sbjct  121  AAAIALLGIFAFYLSFVAELPPPPPKPIKAKKPKERRVRRKK  162


>gi|254773384|ref|ZP_05214900.1| hypothetical protein MaviaA2_01711 [Mycobacterium avium subsp. 
avium ATCC 25291]
Length=277

 Score =  224 bits (571),  Expect = 9e-57, Method: Compositional matrix adjust.
 Identities = 118/140 (85%), Positives = 128/140 (92%), Gaps = 0/140 (0%)

Query  1    MGRKVAVLWHASFSIGAGVLYFYFVLPRWPELMGDTGHSLGTGLRIATGALVGLAALPVV  60
            MGRKVA+ WHASFSI AGVLYF+FVLPRWPELMGDT HSLGT LRI  G LV LAALPVV
Sbjct  1    MGRKVAIPWHASFSIAAGVLYFFFVLPRWPELMGDTTHSLGTALRIVAGVLVALAALPVV  60

Query  61   FTLLRTRKPELGTPQLALSMRIWSIMAHVLAGALIVGTAISEVWLSLDAAGQWLFGIYGA  120
            FTLLRTRKPELGTPQLALS+R+ SI+AHVLAG LI+GTA+SE+WLSLDAAG+WLFGIYGA
Sbjct  61   FTLLRTRKPELGTPQLALSLRVASIVAHVLAGLLIIGTAVSEIWLSLDAAGRWLFGIYGA  120

Query  121  AAAIAVLGFFGFYLSFVAEL  140
            AAAIA+LG F FYLSFVAEL
Sbjct  121  AAAIALLGIFAFYLSFVAEL  140


>gi|336460785|gb|EGO39671.1| hypothetical protein MAPs_37260 [Mycobacterium avium subsp. paratuberculosis 
S397]
Length=277

 Score =  224 bits (570),  Expect = 1e-56, Method: Compositional matrix adjust.
 Identities = 118/140 (85%), Positives = 128/140 (92%), Gaps = 0/140 (0%)

Query  1    MGRKVAVLWHASFSIGAGVLYFYFVLPRWPELMGDTGHSLGTGLRIATGALVGLAALPVV  60
            MGRKVA+ WHASFSI AGVLYF+FVLPRWPELMGDT HSLGT LRI  G LV LAALPVV
Sbjct  1    MGRKVAIPWHASFSIAAGVLYFFFVLPRWPELMGDTTHSLGTALRIVAGVLVALAALPVV  60

Query  61   FTLLRTRKPELGTPQLALSMRIWSIMAHVLAGALIVGTAISEVWLSLDAAGQWLFGIYGA  120
            FTLLRTRKPELGTPQLALS+R+ SI+AHVLAG LI+GTA+SE+WLSLDAAG+WLFGIYGA
Sbjct  61   FTLLRTRKPELGTPQLALSLRVASIVAHVLAGLLIIGTAVSEIWLSLDAAGRWLFGIYGA  120

Query  121  AAAIAVLGFFGFYLSFVAEL  140
            AAAIA+LG F FYLSFVAEL
Sbjct  121  AAAIALLGIFAFYLSFVAEL  140


>gi|41406422|ref|NP_959258.1| hypothetical protein MAP0324 [Mycobacterium avium subsp. paratuberculosis 
K-10]
 gi|41394771|gb|AAS02641.1| hypothetical protein MAP_0324 [Mycobacterium avium subsp. paratuberculosis 
K-10]
Length=277

 Score =  224 bits (570),  Expect = 1e-56, Method: Compositional matrix adjust.
 Identities = 118/140 (85%), Positives = 128/140 (92%), Gaps = 0/140 (0%)

Query  1    MGRKVAVLWHASFSIGAGVLYFYFVLPRWPELMGDTGHSLGTGLRIATGALVGLAALPVV  60
            MGRKVA+ WHASFSI AGVLYF+FVLPRWPELMGDT HSLGT LRI  G LV LAALPVV
Sbjct  1    MGRKVAIPWHASFSIAAGVLYFFFVLPRWPELMGDTTHSLGTALRIVAGVLVALAALPVV  60

Query  61   FTLLRTRKPELGTPQLALSMRIWSIMAHVLAGALIVGTAISEVWLSLDAAGQWLFGIYGA  120
            FTLLRTRKPELGTPQLALS+R+ SI+AHVLAG LI+GTA+SE+WLSLDAAG+WLFGIYGA
Sbjct  61   FTLLRTRKPELGTPQLALSLRVASIVAHVLAGLLIIGTAVSEIWLSLDAAGRWLFGIYGA  120

Query  121  AAAIAVLGFFGFYLSFVAEL  140
            AAAIA+LG F FYLSFVAEL
Sbjct  121  AAAIALLGIFAFYLSFVAEL  140


>gi|118470427|ref|YP_890506.1| hypothetical protein MSMEG_6288 [Mycobacterium smegmatis str. 
MC2 155]
 gi|118171714|gb|ABK72610.1| conserved hypothetical protein [Mycobacterium smegmatis str. 
MC2 155]
Length=293

 Score =  214 bits (546),  Expect = 8e-54, Method: Compositional matrix adjust.
 Identities = 145/274 (53%), Positives = 180/274 (66%), Gaps = 37/274 (13%)

Query  1    MGRKVAVLWHASFSIGAGVLYFYFVLPRWPELMGDTGHSLGTGLRIATGALVGLAALPVV  60
            MGR VAV WH + ++ A  L+F+FV+PRWPELMG+T  +LGT LRI TG LV L ALPVV
Sbjct  1    MGRTVAVTWHVASTLIAAGLFFFFVIPRWPELMGETSPTLGTVLRIVTGVLVALTALPVV  60

Query  61   FTLLRTRKPELGTPQLALSMRIWSIMAHVLAGALIVGTAISEVWLSLDAAGQWLFGIYGA  120
            FTLLRTR+PELGTP+LAL +R  SI+ HVLAG LIVGTAI+E+W+SLD+AG WLFGIYGA
Sbjct  61   FTLLRTRRPELGTPELALRLRTTSIVFHVLAGVLIVGTAIAEIWVSLDSAGSWLFGIYGA  120

Query  121  AAAIAVLGFFGFYLSFVAELPPPPPKPLKPKKPKQRRLRR--KKTAKGDEAEPEAAEEAE  178
            AAA+A+LG FGFYL+F+AELPPPPPKP+KPK    RR  R  K TA  DE + +AA+E E
Sbjct  121  AAALALLGAFGFYLAFIAELPPPPPKPVKPKVNAVRRNTRAAKDTADADEIDKDAADEDE  180

Query  179  --------------------------NTELAAQEDEEAVEAPPESIESPGG--------E  204
                                      + E A Q D+E  +A  E+ + P          E
Sbjct  181  AGEGEDATERAEDSADEPSDDTAVEASDEDADQTDDETEDATSENNDEPRAAEPESKAVE  240

Query  205  PESATREAPAAETATAEEPRGG-LRNRRPTGKTS  237
            PES   EA + + A  ++  GG LRNRRP+ K +
Sbjct  241  PESTAVEAESLDGADTDDSSGGKLRNRRPSNKAN  274


>gi|126437842|ref|YP_001073533.1| hypothetical protein Mjls_5279 [Mycobacterium sp. JLS]
 gi|126237642|gb|ABO01043.1| conserved hypothetical protein [Mycobacterium sp. JLS]
Length=252

 Score =  202 bits (513),  Expect = 5e-50, Method: Compositional matrix adjust.
 Identities = 140/241 (59%), Positives = 166/241 (69%), Gaps = 13/241 (5%)

Query  1    MGRKVAVLWHASFSIGAGVLYFYFVLPRWPELMGDTGHSLGTGLRIATGALVGLAALPVV  60
            M R VAV+WHASFS+ A VLYF+FVLPRW ELMG T  +LGT LRI T AL+GLAALPVV
Sbjct  1    MRRSVAVIWHASFSVIAAVLYFFFVLPRWFELMGSTSPTLGTVLRIVTAALIGLAALPVV  60

Query  61   FTLLRTRKPELGTPQLALSMRIWSIMAHVLAGALIVGTAISEVWLSLDAAGQWLFGIYGA  120
            FTLLRTR+PE GTPQLAL +R  SI  HVLAG LI+G AISE+WL LD+AG WLFGIYGA
Sbjct  61   FTLLRTRRPEFGTPQLALRLRTVSIALHVLAGVLILGAAISEIWLDLDSAGPWLFGIYGA  120

Query  121  AAAIAVLGFFGFYLSFVAELPPPPPKPLKPKKPKQRRLRRKKTAKGDEAEPEAAEEAENT  180
            AAAIA+LG F FYL+FVAE  PPPP      K + RR RR    K  EA+PE   ++E+T
Sbjct  121  AAAIALLGIFAFYLAFVAE-LPPPPPKPLKPKAQTRRGRR----KAQEADPEDTADSEDT  175

Query  181  ELAAQEDEEAV---EAPPESIESPGGEPESATREAPAAETATAEEP---RGGLRNRRPTG  234
                 E+ E     +A  ES+++  G  ++   +      A  EEP    GGLRNRRP+G
Sbjct  176  TEETTEEAERSESDDAVTESVDT--GSADTDATDETEETEAADEEPVASSGGLRNRRPSG  233

Query  235  K  235
            K
Sbjct  234  K  234


>gi|118465404|ref|YP_879665.1| hypothetical protein MAV_0378 [Mycobacterium avium 104]
 gi|118166691|gb|ABK67588.1| conserved hypothetical protein [Mycobacterium avium 104]
Length=277

 Score =  202 bits (513),  Expect = 5e-50, Method: Compositional matrix adjust.
 Identities = 117/140 (84%), Positives = 127/140 (91%), Gaps = 0/140 (0%)

Query  1    MGRKVAVLWHASFSIGAGVLYFYFVLPRWPELMGDTGHSLGTGLRIATGALVGLAALPVV  60
            MGRKVA+ WHASFSI AGVLYF+FVLPRWPELMGDT HSLGT LRI  G L  LAALPVV
Sbjct  1    MGRKVAIPWHASFSIAAGVLYFFFVLPRWPELMGDTTHSLGTALRIVAGVLAALAALPVV  60

Query  61   FTLLRTRKPELGTPQLALSMRIWSIMAHVLAGALIVGTAISEVWLSLDAAGQWLFGIYGA  120
            FTLLRTRKPELGTPQLALS+R+ SI+AHVLAG LI+GTA+SE+WLSLDAAG+WLFGIYGA
Sbjct  61   FTLLRTRKPELGTPQLALSLRVASIVAHVLAGLLIIGTAVSEIWLSLDAAGRWLFGIYGA  120

Query  121  AAAIAVLGFFGFYLSFVAEL  140
            AAAIA+LG F FYLSFVAEL
Sbjct  121  AAAIALLGIFAFYLSFVAEL  140


>gi|108801874|ref|YP_642071.1| hypothetical protein Mmcs_4911 [Mycobacterium sp. MCS]
 gi|119871027|ref|YP_940979.1| hypothetical protein Mkms_5000 [Mycobacterium sp. KMS]
 gi|108772293|gb|ABG11015.1| conserved hypothetical protein [Mycobacterium sp. MCS]
 gi|119697116|gb|ABL94189.1| conserved hypothetical protein [Mycobacterium sp. KMS]
Length=251

 Score =  200 bits (509),  Expect = 2e-49, Method: Compositional matrix adjust.
 Identities = 138/238 (58%), Positives = 163/238 (69%), Gaps = 8/238 (3%)

Query  1    MGRKVAVLWHASFSIGAGVLYFYFVLPRWPELMGDTGHSLGTGLRIATGALVGLAALPVV  60
            M R VAV+WHASFS+ A VLYF+FVLPRW ELMG T  +LGT LRI T AL+GLAALPVV
Sbjct  1    MRRSVAVIWHASFSVIAAVLYFFFVLPRWFELMGSTSPTLGTVLRIVTAALIGLAALPVV  60

Query  61   FTLLRTRKPELGTPQLALSMRIWSIMAHVLAGALIVGTAISEVWLSLDAAGQWLFGIYGA  120
            FTLLRTR+PE GTPQLAL +R  SI  HVLAG LI+G AISE+WL LD+AG WLFGIYGA
Sbjct  61   FTLLRTRRPEFGTPQLALRLRTVSIALHVLAGVLILGAAISEIWLDLDSAGPWLFGIYGA  120

Query  121  AAAIAVLGFFGFYLSFVAELPPPPPKPLKPKKPKQRRLRRKKTAKGDEAEPEAAEEAENT  180
            AAAIA+LG F FYL+FVAE  PPPP      K + RR RR    K  EA PE   ++E+T
Sbjct  121  AAAIALLGIFAFYLAFVAE-LPPPPPKPLKPKAQTRRGRR----KAQEAAPEDTADSEDT  175

Query  181  ELAAQEDEEAVEAPP--ESIESPGGEPESATREAPAAETATA-EEPRGGLRNRRPTGK  235
            E   +E E +    P  ES+++  G+ ++A                 GGLRNRRP+GK
Sbjct  176  EETTEEAERSESDDPVTESVDTESGDTDAADETEETEAADEEPVASSGGLRNRRPSGK  233


>gi|333992653|ref|YP_004525267.1| hypothetical protein JDM601_4013 [Mycobacterium sp. JDM601]
 gi|333488621|gb|AEF38013.1| conserved hypothetical protein [Mycobacterium sp. JDM601]
Length=248

 Score =  160 bits (406),  Expect = 1e-37, Method: Compositional matrix adjust.
 Identities = 132/259 (51%), Positives = 158/259 (62%), Gaps = 25/259 (9%)

Query  1    MGRKVAVLWHASFSIGAGVLYFYFVLPRWPELMGDTGHSLGTGLRIATGALVGLAALPVV  60
            M R  A +WHAS S  A +LYF FVLPRW EL G + H LGT +RI  G LV   ALPVV
Sbjct  1    MSRTAATVWHASLSTLAAILYFLFVLPRWWELTGFSPHVLGTVVRIFLGLLVAGTALPVV  60

Query  61   FTLLRTRKPELGTPQLALSMRIWSIMAHVLAGALIVGTAISEVWLSLDAAGQWLFGIYGA  120
             TL   RKPE GTPQL L++   SI+ HV+AG LI GTAISE+WLSLD AG WLFGIYGA
Sbjct  61   ATLRHARKPEYGTPQLTLTLLTGSIVGHVVAGTLIAGTAISEIWLSLDTAGVWLFGIYGA  120

Query  121  AAAIAVLGFFGFYLSFVAELPPPPPKPLKPKKPKQRRLRRKKTAKGDEAEPEAAEEAENT  180
            AAAIA+LG   FYLSFVA +PPPPPKP+KPK  KQ  LRR +  K  + +  AA+  E+ 
Sbjct  121  AAAIALLGIGAFYLSFVAAMPPPPPKPIKPKSEKQSTLRRWRAKKAKKPDAGAADGVEDD  180

Query  181  ELA-----------AQEDEEAVEAPPESIESPGGE--PESATREAPAAETATAEEPRGGL  227
            +             A ED E+ EA  E++E+       ESA  EAP   +  A+E     
Sbjct  181  DTDEDADEDHDGHDADEDAESGEA-LETVEADSATEVTESAGEEAPELASTGAQE-----  234

Query  228  RNRRPTGKTSHRRRRTRSG  246
               RP+GK     RR + G
Sbjct  235  -KLRPSGK-----RRAKKG  247


>gi|169627388|ref|YP_001701037.1| hypothetical protein MAB_0283c [Mycobacterium abscessus ATCC 
19977]
 gi|169239355|emb|CAM60383.1| Conserved hypothetical protein [Mycobacterium abscessus]
Length=216

 Score =  152 bits (385),  Expect = 4e-35, Method: Compositional matrix adjust.
 Identities = 97/205 (48%), Positives = 128/205 (63%), Gaps = 16/205 (7%)

Query  2    GRKVAVLW----HASFSIGAGVLYFYFVLPRWPELMGDTGHSLGTGLRIATGALVGLAAL  57
            GR+ A +     HA+ S+ AG+L+F+FVLPRWPEL G TGH+ GTG+RI  G LV  A  
Sbjct  4    GRRTATIVLTSSHAALSLIAGLLFFFFVLPRWPELNGATGHTFGTGMRIVGGLLVAAAGA  63

Query  58   PVVFTLLRTRKPELGTPQLALSMRIWSIMAHVLAGALIVGTAISEVWLSLDAAGQWLFGI  117
            PV+ +  RTR+PE  TP+LAL++R  S   H LAG LIVGTAI+E+WL LD  G W FG 
Sbjct  64   PVLISWRRTRRPEFATPRLALTLRAASFTGHFLAGVLIVGTAIAEIWLDLDRFGIWEFGG  123

Query  118  YGAAAAIAVLGFFGFYLSFVAELPPPPPKPLKPKKPKQRRLRRKKTAKGDE--------A  169
            YGAAAAI +L F  ++L+FVAE P P   P    +P +++L R  T   D+        +
Sbjct  124  YGAAAAIGILAFAAYHLAFVAERPAPERTP----RPAKKKLTRATTENADDSDVDKAIAS  179

Query  170  EPEAAEEAENTELAAQEDEEAVEAP  194
            EPEA E A+     + + +E V  P
Sbjct  180  EPEAREPADEPSGDSPDSDEKVLEP  204


>gi|341878512|gb|EGT34447.1| CBN-TAG-279 protein [Caenorhabditis brenneri]
Length=1860

 Score = 47.8 bits (112),  Expect = 0.002, Method: Composition-based stats.
 Identities = 37/121 (31%), Positives = 53/121 (44%), Gaps = 15/121 (12%)

Query  141  PPPPPKPLKPKKPKQR-----RLRRKKTAKGDEAEPEAAEEAENTELAAQEDEEAVEAPP  195
            P P P P K KK K++     +  +K +A   +AE   AE+A        +D EA+E P 
Sbjct  818  PAPKPTPAKSKKSKRKTHFGQKRSKKPSANRKKAEDGEAEKAPEANPIEAKDGEAMEVPK  877

Query  196  ---------ESIESPGGEP-ESATREAPAAETATAEEPRGGLRNRRPTGKTSHRRRRTRS  245
                     E++E P   P E+   EA  A  A  +  RGG R ++   K     R + S
Sbjct  878  ANPIEAEDGEAMEVPEANPIEAEDGEAIEAPAAKPKPKRGGRRGKKAVRKQKGNIRSSTS  937

Query  246  G  246
            G
Sbjct  938  G  938


>gi|255284218|ref|ZP_05348773.1| glycogen synthase [Bryantella formatexigens DSM 14469]
 gi|255265243|gb|EET58448.1| glycogen synthase [Bryantella formatexigens DSM 14469]
Length=811

 Score = 37.7 bits (86),  Expect = 1.5, Method: Compositional matrix adjust.
 Identities = 35/97 (37%), Positives = 46/97 (48%), Gaps = 14/97 (14%)

Query  143  PPPKPLKPKKPKQRRLRRKKTAKGDEAEPEAAEEAENTELAAQEDE------EAVEAPPE  196
            P  +P K +KPK +  R  KTAK ++A P  AEE +   + A+E +      EAV+A P 
Sbjct  56   PKAEPAKEEKPKAKTTRAAKTAKTEKAAPVKAEEVKTEPVKAEEPKAKPAKAEAVKAEPA  115

Query  197  SIESPGGEP--------ESATREAPAAETATAEEPRG  225
              E P  E         E A  E    E A AEEP+ 
Sbjct  116  KAEEPKAESVKAEEAKAEPAKTEEAKVEPAKAEEPKA  152



Lambda     K      H
   0.316    0.133    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Effective search space used: 371539772520


  Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
    Posted date:  Sep 5, 2011  4:36 AM
  Number of letters in database: 5,219,829,388
  Number of sequences in database:  15,229,318



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40