BLASTP 2.2.25+
Reference:
Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database
search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Alejandro A. Schäffer, L. Aravind, Thomas L. Madden, Sergei
Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
protein database searches with composition-based statistics and
other refinements", Nucleic Acids Res. 29:2994-3005.
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
15,229,318 sequences; 5,219,829,388 total letters
Query= Rv3817
Length=251
Score E
Sequences producing significant alignments: (Bits) Value
gi|15610953|ref|NP_218334.1| phosphotransferase [Mycobacterium t... 499 2e-139
gi|340628786|ref|YP_004747238.1| putative phosphotransferase [My... 487 8e-136
gi|240172626|ref|ZP_04751285.1| phosphotransferase [Mycobacteriu... 331 5e-89
gi|183985348|ref|YP_001853639.1| phosphotransferase [Mycobacteri... 318 5e-85
gi|118620016|ref|YP_908348.1| phosphotransferase [Mycobacterium ... 313 2e-83
gi|169627268|ref|YP_001700917.1| phosphotransferase [Mycobacteri... 258 6e-67
gi|311897529|dbj|BAJ29937.1| putative aminoglycoside 3'-phosphot... 233 2e-59
gi|296139187|ref|YP_003646430.1| aminoglycoside phosphotransfera... 226 2e-57
gi|271962028|ref|YP_003336224.1| aminoglycoside 3'-phosphotransf... 217 1e-54
gi|332669644|ref|YP_004452652.1| aminoglycoside phosphotransfera... 216 2e-54
gi|323358347|ref|YP_004224743.1| aminoglycoside phosphotransfera... 214 1e-53
gi|297159017|gb|ADI08729.1| phosphotransferase [Streptomyces bin... 213 3e-53
gi|288921649|ref|ZP_06415919.1| aminoglycoside phosphotransferas... 209 4e-52
gi|152965765|ref|YP_001361549.1| aminoglycoside phosphotransfera... 207 1e-51
gi|329938743|ref|ZP_08288139.1| streptomycin 6-phosphotransferas... 206 2e-51
gi|294629440|ref|ZP_06708000.1| kanamycin kinase [Streptomyces s... 203 2e-50
gi|336320062|ref|YP_004600030.1| aminoglycoside phosphotransfera... 195 7e-48
gi|256395310|ref|YP_003116874.1| aminoglycoside phosphotransfera... 190 1e-46
gi|296129035|ref|YP_003636285.1| aminoglycoside phosphotransfera... 180 1e-43
gi|308176109|ref|YP_003915515.1| phosphotransferase [Arthrobacte... 169 3e-40
gi|184200695|ref|YP_001854902.1| hypothetical protein KRH_10490 ... 149 5e-34
gi|297565411|ref|YP_003684383.1| aminoglycoside phosphotransfera... 100 2e-19
gi|327189670|gb|EGE56818.1| putative aminoglycoside 3'-phosphotr... 99.8 3e-19
gi|194365494|ref|YP_002028104.1| aminoglycoside phosphotransfera... 99.0 5e-19
gi|241205653|ref|YP_002976749.1| aminoglycoside phosphotransfera... 96.7 3e-18
gi|344207168|ref|YP_004792309.1| Kanamycin kinase [Burkholderia ... 95.9 5e-18
gi|190892695|ref|YP_001979237.1| aminoglycoside 3'-phosphotransf... 95.1 9e-18
gi|297154663|gb|ADI04375.1| phosphotransferase [Streptomyces bin... 94.4 1e-17
gi|254524099|ref|ZP_05136154.1| aminoglycoside phosphotransferas... 94.4 1e-17
gi|312191071|gb|ADQ43421.1| aminoglycoside 3'-phosphotransferase... 94.4 1e-17
gi|86358549|ref|YP_470441.1| aminoglycoside 3`-phosphotransferas... 94.0 2e-17
gi|226357205|ref|YP_002786945.1| kanamycin kinase (aminoglycosid... 92.8 4e-17
gi|315271315|gb|ACO47191.2| putative kanamycin kinase (aminoglyc... 92.4 5e-17
gi|62857277|dbj|BAD95814.1| aminoglycoside 3'-phosphotransferase... 91.3 1e-16
gi|15805107|ref|NP_293792.1| aminoglycoside 3`-phosphotransferas... 90.9 2e-16
gi|320332959|ref|YP_004169670.1| aminoglycoside phosphotransfera... 89.4 4e-16
gi|78063044|ref|YP_372952.1| aminoglycoside phosphotransferase [... 89.0 6e-16
gi|107103636|ref|ZP_01367554.1| hypothetical protein PaerPA_0100... 88.6 8e-16
gi|254242804|ref|ZP_04936126.1| aminoglycoside 3'-phosphotransfe... 88.6 8e-16
gi|222086539|ref|YP_002545073.1| aminoglycoside 3-phosphotransfe... 88.6 8e-16
gi|83999850|emb|CAH60150.1| putative phosphotransferase [Strepto... 88.6 9e-16
gi|85813916|emb|CAF31545.1| putative fortimicin 3'-phosphotransf... 88.6 9e-16
gi|254237006|ref|ZP_04930329.1| aminoglycoside 3'-phosphotransfe... 87.8 1e-15
gi|125466|sp|P00555.1|KKA5_STRFR RecName: Full=Aminoglycoside 3'... 87.8 1e-15
gi|953191|emb|CAA62365.1| aminoglycoside phosphotransferase (3')... 87.0 2e-15
gi|190574079|ref|YP_001971924.1| putative aminoglycoside 3'-phos... 87.0 2e-15
gi|15599314|ref|NP_252808.1| aminoglycoside 3'-phosphotransferas... 87.0 2e-15
gi|218662247|ref|ZP_03518177.1| putative aminoglycoside 3'-phosp... 86.7 3e-15
gi|116253139|ref|YP_768977.1| aminoglycoside 3'-phosphotransfera... 86.7 3e-15
gi|15982219|emb|CAC86252.1| kanomycin resistance protein [Clonin... 86.3 4e-15
>gi|15610953|ref|NP_218334.1| phosphotransferase [Mycobacterium tuberculosis H37Rv]
gi|15843441|ref|NP_338478.1| aminoglycoside 3'-phosphotransferase [Mycobacterium tuberculosis
CDC1551]
gi|31794991|ref|NP_857484.1| putative phosphotransferase [Mycobacterium bovis AF2122/97]
78 more sequence titles
Length=251
Score = 499 bits (1284), Expect = 2e-139, Method: Compositional matrix adjust.
Identities = 250/251 (99%), Positives = 251/251 (100%), Gaps = 0/251 (0%)
Query 1 VSFPSSPPALPAIVARFAVGRPVRAVWVNELGGVTFRVDSGMGAGCEFIKVARRGTADFA 60
+SFPSSPPALPAIVARFAVGRPVRAVWVNELGGVTFRVDSGMGAGCEFIKVARRGTADFA
Sbjct 1 MSFPSSPPALPAIVARFAVGRPVRAVWVNELGGVTFRVDSGMGAGCEFIKVARRGTADFA 60
Query 61 NEARRLRWAAPYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGL 120
NEARRLRWAAPYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGL
Sbjct 61 NEARRLRWAAPYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGL 120
Query 121 RTLHDSLPVHSCPFDWSTASRLAKLAPARRAELGDSPPVDRLVVCHGDACSPNTILDDTG 180
RTLHDSLPVHSCPFDWSTASRLAKLAPARRAELGDSPPVDRLVVCHGDACSPNTILDDTG
Sbjct 121 RTLHDSLPVHSCPFDWSTASRLAKLAPARRAELGDSPPVDRLVVCHGDACSPNTILDDTG 180
Query 181 RCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVRDDEFFAAYGVAPDPARIDYYR 240
RCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVRDDEFFAAYGVAPDPARIDYYR
Sbjct 181 RCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVRDDEFFAAYGVAPDPARIDYYR 240
Query 241 RLWQAEDDSSR 251
RLWQAEDDSSR
Sbjct 241 RLWQAEDDSSR 251
>gi|340628786|ref|YP_004747238.1| putative phosphotransferase [Mycobacterium canettii CIPT 140010059]
gi|340006976|emb|CCC46167.1| putative phosphotransferase [Mycobacterium canettii CIPT 140010059]
Length=251
Score = 487 bits (1253), Expect = 8e-136, Method: Compositional matrix adjust.
Identities = 245/251 (98%), Positives = 249/251 (99%), Gaps = 0/251 (0%)
Query 1 VSFPSSPPALPAIVARFAVGRPVRAVWVNELGGVTFRVDSGMGAGCEFIKVARRGTADFA 60
+SFPSSPPALPAIVARFAVGRPVRAVWVNELGGVTFRVDSG+GAG EFIKVARRGTADFA
Sbjct 1 MSFPSSPPALPAIVARFAVGRPVRAVWVNELGGVTFRVDSGIGAGSEFIKVARRGTADFA 60
Query 61 NEARRLRWAAPYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGL 120
NEARRLRWAAPYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGL
Sbjct 61 NEARRLRWAAPYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGL 120
Query 121 RTLHDSLPVHSCPFDWSTASRLAKLAPARRAELGDSPPVDRLVVCHGDACSPNTILDDTG 180
RTLHDSLPV SCPFDWSTASRLAKLAPARRAELGDSPPVDRLVVCHGDACSPNTILDDTG
Sbjct 121 RTLHDSLPVPSCPFDWSTASRLAKLAPARRAELGDSPPVDRLVVCHGDACSPNTILDDTG 180
Query 181 RCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVRDDEFFAAYGVAPDPARIDYYR 240
RCCGHVDFG+LGVADRWADLAVATLSLQWNFPDYPGQ+RDDEFFAAYGVAPDPARIDYYR
Sbjct 181 RCCGHVDFGDLGVADRWADLAVATLSLQWNFPDYPGQLRDDEFFAAYGVAPDPARIDYYR 240
Query 241 RLWQAEDDSSR 251
RLWQAEDDSSR
Sbjct 241 RLWQAEDDSSR 251
>gi|240172626|ref|ZP_04751285.1| phosphotransferase [Mycobacterium kansasii ATCC 12478]
Length=235
Score = 331 bits (849), Expect = 5e-89, Method: Compositional matrix adjust.
Identities = 170/238 (72%), Positives = 190/238 (80%), Gaps = 7/238 (2%)
Query 10 LPAIVARFAVGRPVRAVWVNELGGVTFRVDSGMGAGCEFIKVARRGTADFANEARRLRWA 69
+PA+V RFA GR VRAVW NELGGVTFR+ G G EF+KVA GT DFA+EARRLRWA
Sbjct 1 MPAVVRRFAAGRRVRAVWTNELGGVTFRI----GPGTEFVKVAGVGTVDFADEARRLRWA 56
Query 70 APYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGLRTLHDSLPV 129
A Y VP VLG G+DGD AWL T LPG+SAVHPRW A+P VAV A+ GLRTLHD+LPV
Sbjct 57 AQYTPVPSVLGFGLDGDRAWLRTGGLPGVSAVHPRWLAAPDVAVRAIAEGLRTLHDTLPV 116
Query 130 HSCPFDWSTASRLAKLAPARRAELGDSPPVDRLVVCHGDACSPNTILDDTGRCCGHVDFG 189
SCPFDWS A RLA+L+PA RA+LGD PPVDR+VVCHGDACSPNT++D+ GRCCGHVD
Sbjct 117 SSCPFDWSAAGRLARLSPAWRAKLGDPPPVDRVVVCHGDACSPNTLIDEHGRCCGHVDVD 176
Query 190 NLGVADRWADLAVATLSLQWNFPDYPGQVRDDEFFAAYGVAPDPARIDYYRRLWQAED 247
+GVADRWADLAVATLSL WN YPG+ D FF AYGV PDP R+DYYRRLWQ ED
Sbjct 177 AMGVADRWADLAVATLSLGWN---YPGRGWDTMFFEAYGVEPDPPRLDYYRRLWQTED 231
>gi|183985348|ref|YP_001853639.1| phosphotransferase [Mycobacterium marinum M]
gi|183178674|gb|ACC43784.1| phosphotransferase [Mycobacterium marinum M]
Length=244
Score = 318 bits (815), Expect = 5e-85, Method: Compositional matrix adjust.
Identities = 165/236 (70%), Positives = 184/236 (78%), Gaps = 8/236 (3%)
Query 16 RFAVGRPVRAVWVNELGGVTFRVDSGMGAGCEFIKVARRGTADFANEARRLRWAAPYLAV 75
R A GRPV+AVW NELGGVTFRV G G EFIKVAR G DF EA+RLRWA+ +L V
Sbjct 16 RLAAGRPVQAVWANELGGVTFRV----GPGAEFIKVARTGVFDFTAEAQRLRWASAHLTV 71
Query 76 PRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGLRTLHDSLPVHSCPFD 135
P VL GV+ DWAWL T LPG+SAVHPRW A+P VAV A+G GLRTLH+ LPV +CPFD
Sbjct 72 PAVLDFGVEQDWAWLRTAGLPGVSAVHPRWVAAPDVAVRAIGVGLRTLHERLPVRACPFD 131
Query 136 WSTASRLAKLAPARRAELGDSPPVDRLVVCHGDACSPNTILDDTGRCCGHVDFGNLGVAD 195
WS SRLA+L A RA +G PP+DRLVVCHGDAC+PNT++ + G CCGHVD G LGVAD
Sbjct 132 WSAQSRLARLTAAGRANVGAPPPIDRLVVCHGDACAPNTLIAEDGNCCGHVDLGTLGVAD 191
Query 196 RWADLAVATLSLQWNFPDYPGQVRDDEFFAAYGVAPDPARIDYYRRLWQAED-DSS 250
RWADLAVATLSLQWNF PG + +FFAAYGVAPDP RIDYYRRLW AED DSS
Sbjct 192 RWADLAVATLSLQWNF---PGHGWEAKFFAAYGVAPDPRRIDYYRRLWGAEDLDSS 244
>gi|118620016|ref|YP_908348.1| phosphotransferase [Mycobacterium ulcerans Agy99]
gi|118572126|gb|ABL06877.1| phosphotransferase [Mycobacterium ulcerans Agy99]
Length=276
Score = 313 bits (801), Expect = 2e-83, Method: Compositional matrix adjust.
Identities = 158/228 (70%), Positives = 178/228 (79%), Gaps = 7/228 (3%)
Query 16 RFAVGRPVRAVWVNELGGVTFRVDSGMGAGCEFIKVARRGTADFANEARRLRWAAPYLAV 75
R A GRPV+AVW NELGGVTFRV G G EFIKVAR G DF EA+RLRWA+ +L V
Sbjct 16 RLAAGRPVQAVWANELGGVTFRV----GPGAEFIKVARTGVFDFTAEAQRLRWASAHLTV 71
Query 76 PRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGLRTLHDSLPVHSCPFD 135
P VL GV+ DWAWL T LPG+SAVHPRW A+P VAV A+G GLRTLH+ LPV +CPFD
Sbjct 72 PAVLDFGVEQDWAWLRTAGLPGVSAVHPRWVAAPDVAVRAIGVGLRTLHERLPVRACPFD 131
Query 136 WSTASRLAKLAPARRAELGDSPPVDRLVVCHGDACSPNTILDDTGRCCGHVDFGNLGVAD 195
WS SRLA+L A RA +G PP+DRLVVCHGDAC+P+T++ + G CCGHVD G LGVAD
Sbjct 132 WSAQSRLARLTAAGRANVGAPPPIDRLVVCHGDACAPHTLIAEDGNCCGHVDLGTLGVAD 191
Query 196 RWADLAVATLSLQWNFPDYPGQVRDDEFFAAYGVAPDPARIDYYRRLW 243
RWADLAVATLSLQWNF PG + +FFAAYGVAPDP RIDYYRRLW
Sbjct 192 RWADLAVATLSLQWNF---PGHGWEAKFFAAYGVAPDPRRIDYYRRLW 236
>gi|169627268|ref|YP_001700917.1| phosphotransferase [Mycobacterium abscessus ATCC 19977]
gi|169239235|emb|CAM60263.1| Probable phosphotransferase [Mycobacterium abscessus]
Length=240
Score = 258 bits (659), Expect = 6e-67, Method: Compositional matrix adjust.
Identities = 135/248 (55%), Positives = 170/248 (69%), Gaps = 9/248 (3%)
Query 1 VSFPSSPPALPAIVARFAVGRPVRAVWVNELGGVTFRVDSGMGAGCEFIKV-ARRGTADF 59
++ P+ P +P IVA + GRPV AVW N++GG TF++ G GA EF+KV A +
Sbjct 1 MTIPTEPVTIPDIVAHLSAGRPVLAVWHNQIGGHTFQI--GSGADREFVKVSAPHPAINL 58
Query 60 ANEARRLRWAAPYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAG 119
EA RLRWA Y VP VL V +G WLHT +PG SAV PRW +P+V+V A+ +G
Sbjct 59 PAEAERLRWARRYTTVPTVLSVNTEGPLHWLHTAGIPGDSAVAPRWIENPEVSVRAIASG 118
Query 120 LRTLHDSLPVHSCPFDWSTASRLAKLAPARRAELGDSPPVDRLVVCHGDACSPNTILDDT 179
LR LHD LPV CP+ WS A+RLA++ L + P +DRLVVCHGDAC+PNT+LD
Sbjct 119 LRALHDRLPVVDCPYSWSVAARLARI--PNPGGLSEHPAIDRLVVCHGDACAPNTLLDGN 176
Query 180 GRCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVRDDEFFAAYGVAPDPARIDYY 239
G CGHVDFG+LGVADRWADLAVA+ SL+WN+ G+ +D FFAAYGV DP R+ YY
Sbjct 177 GNWCGHVDFGDLGVADRWADLAVASWSLEWNY----GKGWNDAFFAAYGVTEDPERMAYY 232
Query 240 RRLWQAED 247
RRLW +E+
Sbjct 233 RRLWDSEE 240
>gi|311897529|dbj|BAJ29937.1| putative aminoglycoside 3'-phosphotransferase [Kitasatospora
setae KM-6054]
Length=776
Score = 233 bits (595), Expect = 2e-59, Method: Compositional matrix adjust.
Identities = 136/260 (53%), Positives = 158/260 (61%), Gaps = 26/260 (10%)
Query 4 PSSPPALPAIVARFAVGRPVRAVWVNELGGVTFRVDSGMGAGCEFIKVARRGTA-DFANE 62
P +PA V AVG P+ AVW NEL GVTFR+ G G G F K A G+ D A E
Sbjct 520 PEGDTEIPAAVVELAVGEPIAAVWRNELKGVTFRL--GDGPGRRFAKWAPAGSGVDLAVE 577
Query 63 ARRLRWAAPYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGLRT 122
A R RWA P+ AVP VLG+G D +WL T LPG SAV P RA P+ AV A+G GLR
Sbjct 578 AERTRWARPFTAVPEVLGLGGDEHGSWLLTAGLPGRSAVEPELRADPETAVRAIGEGLRA 637
Query 123 LHDSLPVHSCPFDWSTASRLAKLAPARR-------------------AELGDSPPVDRLV 163
LH++LPV CP++WSTA R+A+ A RR A L D PPVDR V
Sbjct 638 LHEALPVADCPYEWSTAERVARAAELRRPPEEWYPALRHHGTVERALAVLADPPPVDRAV 697
Query 164 VCHGDACSPNTILDDTGRCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVRDDEF 223
VCHGD C+PNT++ D GR GHVD G LGVADRWADLAVA+ S WN+ G +
Sbjct 698 VCHGDPCAPNTLVGDDGRPSGHVDLGALGVADRWADLAVASWSTVWNY----GPGWELPL 753
Query 224 FAAYGVAPDPARIDYYRRLW 243
AAYGV PD R+DYYR LW
Sbjct 754 LAAYGVEPDAERLDYYRLLW 773
>gi|296139187|ref|YP_003646430.1| aminoglycoside phosphotransferase [Tsukamurella paurometabola
DSM 20162]
gi|296027321|gb|ADG78091.1| aminoglycoside phosphotransferase [Tsukamurella paurometabola
DSM 20162]
Length=261
Score = 226 bits (576), Expect = 2e-57, Method: Compositional matrix adjust.
Identities = 125/224 (56%), Positives = 152/224 (68%), Gaps = 11/224 (4%)
Query 23 VRAVWVNELGGVTFRVDSGMGAGCEFIKVARRGT--ADFANEARRLRWAAPYLAVPRVLG 80
+ VW N+LGG+TFR+ E+IK T DF EA RLRWA P++ VPRV+
Sbjct 23 ITPVWRNDLGGITFRLGDD-----EYIKWMPTQTPELDFDAEAVRLRWAQPHVTVPRVIA 77
Query 81 VGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGLRTLHDSLPVHSCPFDWSTAS 140
G WL T A+ G SA+ PRW SP +A A+GAGLR LHD+LPVH CPFDWS ++
Sbjct 78 RGSSDGAEWLVTAAIAGTSAIDPRWADSPAIAARAIGAGLRHLHDTLPVHVCPFDWSVSA 137
Query 141 RLAKLAPARRAELGDSPPVDRLVVCHGDACSPNTILDDTGRCCGHVDFGNLGVADRWADL 200
R L PA RA LG +PP+DRLVVCHGDAC+PNT++ + GR GHVD G+LG ADRWADL
Sbjct 138 RTVGLTPAERAALGPTPPIDRLVVCHGDACAPNTLVGEDGRFAGHVDLGSLGTADRWADL 197
Query 201 AVATLSLQWNFPDYPGQVRDDEFFAAYGVAPDPARIDYYRRLWQ 244
AVA +SL WNF Q R D F AYGVAPD AR+ +YRR+W+
Sbjct 198 AVAAMSLDWNF----DQPRPDVLFEAYGVAPDEARLAFYRRVWK 237
>gi|271962028|ref|YP_003336224.1| aminoglycoside 3'-phosphotransferase [Streptosporangium roseum
DSM 43021]
gi|270505203|gb|ACZ83481.1| aminoglycoside 3'-phosphotransferase [Streptosporangium roseum
DSM 43021]
Length=270
Score = 217 bits (553), Expect = 1e-54, Method: Compositional matrix adjust.
Identities = 134/268 (50%), Positives = 156/268 (59%), Gaps = 32/268 (11%)
Query 2 SFPSSPPALPAIVARFAVGRPVRAVWVNELGGVTFRVDSGMGAGCEFIKVARRGT--ADF 59
P+ P +P +VA A G V VW NELGG+TFR++ G G G + K GT D
Sbjct 8 EIPTGPVTVPDVVAALAGGDTVTPVWRNELGGLTFRLEDGRG-GTRYAKWIAAGTPEIDL 66
Query 60 ANEARRLRWAAPYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAG 119
EA RL WA + VPRV+ G D D AWL T+A+PG SAV PRW A P A A+G G
Sbjct 67 PAEAERLAWAQRWATVPRVIEHGTDADGAWLVTEAVPGRSAVDPRWIADPATAAAAIGRG 126
Query 120 LRTLHDSLPVHSCPFDWSTASRLAK----------------------LAPARRAELGDSP 157
LR LHD LPV CPFDWS RLA+ LA A RA +G+ P
Sbjct 127 LRLLHDVLPVEQCPFDWSIDRRLARADERIADGEEPAGWSPEHRHLDLAKA-RARIGEPP 185
Query 158 PVDRLVVCHGDACSPNTILDDTGRCCGHVDFGNLGVADRWADLAVATLSLQWNF-PDYPG 216
+DRLVVCHGDAC PNT+L D G HVD G+LGVADRWADLAV S +WN+ P Y G
Sbjct 186 AIDRLVVCHGDACVPNTLLHDDGTFAAHVDLGSLGVADRWADLAVTAWSTEWNYGPGYDG 245
Query 217 QVRDDEFFAAYGVAPDPARIDYYRRLWQ 244
+ D AYG+ PDP RI YYR LW
Sbjct 246 ILYD-----AYGITPDPERIAYYRLLWD 268
>gi|332669644|ref|YP_004452652.1| aminoglycoside phosphotransferase [Cellulomonas fimi ATCC 484]
gi|332338682|gb|AEE45265.1| aminoglycoside phosphotransferase [Cellulomonas fimi ATCC 484]
Length=257
Score = 216 bits (551), Expect = 2e-54, Method: Compositional matrix adjust.
Identities = 133/250 (54%), Positives = 151/250 (61%), Gaps = 19/250 (7%)
Query 4 PSSPPALPAIVARFAVGRPVRAVWVNELGGVTFRVDSGMGAGCEFIKVARRGT-ADFANE 62
P P +P VA R V VW NELGG+TF +D F+K G D E
Sbjct 18 PVDPAVVPRAVAGMGGVREV--VWRNELGGLTFLLDEPE----RFVKWVPTGLPLDLDAE 71
Query 63 ARRLRWAAPYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGLRT 122
RLRWAA + VP VL G D + AW+ T L G SAV PRW P AV A+GAGLR
Sbjct 72 VVRLRWAARFTPVPTVLEAGADDEGAWIVTHPLAGRSAVDPRWLDEPLTAVRAIGAGLRA 131
Query 123 LHDSLPVHSCPFDWSTASRLAKLAPAR-------RAELGDSPPVDRLVVCHGDACSPNTI 175
+HD+LP+ CPFDW RLA +A AR RA LG+ PPVDRLVVCHGDAC+PNT+
Sbjct 132 MHDALPLDGCPFDWGVPRRLA-VADAREGGRSPERAALGEPPPVDRLVVCHGDACAPNTL 190
Query 176 LDDTGRCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVRDDEFFAAYGVAPDPAR 235
L D G HVD G LGVADRWADLAVAT S WN+ G ++ AYGVAPDPAR
Sbjct 191 LADDGSWLAHVDLGALGVADRWADLAVATYSTLWNY----GPGYEEPLLDAYGVAPDPAR 246
Query 236 IDYYRRLWQA 245
DYYRRLW A
Sbjct 247 TDYYRRLWDA 256
>gi|323358347|ref|YP_004224743.1| aminoglycoside phosphotransferase [Microbacterium testaceum StLB037]
gi|323274718|dbj|BAJ74863.1| aminoglycoside phosphotransferase [Microbacterium testaceum StLB037]
Length=244
Score = 214 bits (544), Expect = 1e-53, Method: Compositional matrix adjust.
Identities = 122/248 (50%), Positives = 147/248 (60%), Gaps = 10/248 (4%)
Query 1 VSFPSSPPALPAIVARFAVGRPVRAVWVNELGGVTFRVDSGMGAGCEFIKVA-RRGTADF 59
+S P++ +P V A + VW N LGG+TFR + G F+K R
Sbjct 1 MSIPAASEPVPGRVRELAGDAHIEPVWRNALGGLTFR--AAGADGIRFVKYGPRNAETSM 58
Query 60 ANEARRLRWAAPYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAG 119
EA RLRWA+ + VPR L G D WL T+A+PGLSAV PRW A P AV A+G G
Sbjct 59 RAEAERLRWASAFTPVPRALDEGRDATHEWLVTEAVPGLSAVDPRWIADPARAVRAVGRG 118
Query 120 LRTLHDSLPVHSCPFDWSTASRLAKLAP---ARRAELGDSPPVDRLVVCHGDACSPNTIL 176
LR LHD+LPV CPF W+ RLA A EL D+PP+DRLVVCHGDAC+PNT+L
Sbjct 119 LRALHDALPVAECPFSWTVPDRLANAAGRGIQVPVELYDAPPIDRLVVCHGDACAPNTLL 178
Query 177 DDTGRCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVRDDEFFAAYGVAPDPARI 236
D G HVD G LGVADRWADLA ATLS WN+ G ++ AYGVAPD R+
Sbjct 179 GDDGEWTAHVDLGALGVADRWADLAAATLSTGWNY----GPGWEEALLTAYGVAPDATRL 234
Query 237 DYYRRLWQ 244
+Y+ LW
Sbjct 235 AFYQALWN 242
>gi|297159017|gb|ADI08729.1| phosphotransferase [Streptomyces bingchenggensis BCW-1]
Length=222
Score = 213 bits (541), Expect = 3e-53, Method: Compositional matrix adjust.
Identities = 121/224 (55%), Positives = 145/224 (65%), Gaps = 10/224 (4%)
Query 26 VWVNELGGVTFRVDSGMGAGCEFIKVARRGT-ADFANEARRLRWAAPYLAVPRVLGVGVD 84
VW+NELGG+TF++ G G F K +G+ AD EA RLRWA Y VP+VL +G D
Sbjct 4 VWLNELGGLTFQL--GSGDDRLFAKWDPKGSGADLEVEATRLRWARRYTPVPQVLDLGAD 61
Query 85 GDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGLRTLHDSLPVHSCPFDWSTASRL-- 142
D W+ T LPG SAV PRW+A P+ AV ALG LR LH+ LPV CPF WS RL
Sbjct 62 EDGTWMITRGLPGESAVSPRWKADPRTAVRALGTALRALHERLPVDDCPFSWSVDHRLDV 121
Query 143 AKLAPARR-AELGDSPPVDRLVVCHGDACSPNTILDDTGRCCGHVDFGNLGVADRWADLA 201
A+ A A A+L PP+D LVVCHGD C+PNT+L+ G HVD G LG+ADRWADLA
Sbjct 122 ARRAGAEVPADLPAPPPIDHLVVCHGDPCAPNTLLNSDGTWSAHVDMGALGLADRWADLA 181
Query 202 VATLSLQWNFPDYPGQVRDDEFFAAYGVAPDPARIDYYRRLWQA 245
VAT S +WN+ G DD YG++PDP RID+YRRLW A
Sbjct 182 VATWSTEWNY----GPGWDDTLLDGYGISPDPERIDFYRRLWAA 221
>gi|288921649|ref|ZP_06415919.1| aminoglycoside phosphotransferase [Frankia sp. EUN1f]
gi|288346969|gb|EFC81276.1| aminoglycoside phosphotransferase [Frankia sp. EUN1f]
Length=242
Score = 209 bits (531), Expect = 4e-52, Method: Compositional matrix adjust.
Identities = 127/245 (52%), Positives = 151/245 (62%), Gaps = 16/245 (6%)
Query 10 LPAIVARFAVGRPVRAVWVNELGGVTFRVDSGMGAGCEFIKVARRGT-ADFANEARRLRW 68
+P+ + A G VR VW NELGG+TF + AG F+K A GT + EA RLRW
Sbjct 1 MPSAIVAMAAGNRVRPVWRNELGGLTFEMTGP--AGRRFVKWAPAGTHLNLMAEASRLRW 58
Query 69 AAPYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGLRTLHDSLP 128
AAP+ VP VLG G DG +WL T LPG +AVH W+A P AV A+G GLR H++LP
Sbjct 59 AAPFTPVPAVLGEGRDGAGSWLITAGLPGRNAVHAIWKAEPLRAVQAIGRGLRAFHEALP 118
Query 129 VHSCPFDWSTASRLAK------LAPARR---AELGDSPPVDRLVVCHGDACSPNTILDDT 179
V CPF WS A RLA+ A RR D+P VD LVVC GDAC+PNT+L +
Sbjct 119 VAECPFSWSVADRLAEASARATTAQHRRRLADLAADAPAVDVLVVCQGDACAPNTLLGED 178
Query 180 GRCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVRDDEFFAAYGVAPDPARIDYY 239
GR GHVD G LGVADRWADLAVAT S +WN+ G + AYGVAPD R +Y
Sbjct 179 GRWSGHVDLGALGVADRWADLAVATWSTEWNY----GPGWEHVLLDAYGVAPDDDRTAFY 234
Query 240 RRLWQ 244
RRLW+
Sbjct 235 RRLWE 239
>gi|152965765|ref|YP_001361549.1| aminoglycoside phosphotransferase [Kineococcus radiotolerans
SRS30216]
gi|151360282|gb|ABS03285.1| aminoglycoside phosphotransferase [Kineococcus radiotolerans
SRS30216]
Length=256
Score = 207 bits (527), Expect = 1e-51, Method: Compositional matrix adjust.
Identities = 125/223 (57%), Positives = 141/223 (64%), Gaps = 13/223 (5%)
Query 25 AVWVNELGGVTFRV-DSGMGAGCEFIKVARRGTA-DFANEARRLRWAAPYLAVPRVLGVG 82
A WVN GG TFR+ D G+ F K A G D A EA R RWA +LAVP VL G
Sbjct 41 AAWVNAAGGTTFRLEDRGV-----FCKWAPAGVGLDLAAEADRSRWAVRWLAVPEVLEHG 95
Query 83 VDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGLRTLHDSLPVHSCPFDWSTASRL 142
+ WL T LPG SAV WRA P+ AVPAL GLR LHD+LPV CPFDWS RL
Sbjct 96 TCPEGEWLVTRGLPGRSAVAEDWRARPEAAVPALARGLRRLHDTLPVAQCPFDWSVPHRL 155
Query 143 AKLAPARRAELGDSPPVDRLVVCHGDACSPNTIL-DDTGRCCGHVDFGNLGVADRWADLA 201
A+ A L + PPVDRLVVCHGDAC+PNT+L + +G HVD G LGVADRWADLA
Sbjct 156 ARAVGAVEGLL-EPPPVDRLVVCHGDACAPNTLLAEGSGEAVAHVDLGALGVADRWADLA 214
Query 202 VATLSLQWNFPDYPGQVRDDEFFAAYGVAPDPARIDYYRRLWQ 244
VATLSL WN+ G + + AAYGV PDP R YYR LW+
Sbjct 215 VATLSLGWNY----GPGWEGTYLAAYGVDPDPVRTAYYRALWE 253
>gi|329938743|ref|ZP_08288139.1| streptomycin 6-phosphotransferase [Streptomyces griseoaurantiacus
M045]
gi|329302234|gb|EGG46126.1| streptomycin 6-phosphotransferase [Streptomyces griseoaurantiacus
M045]
Length=252
Score = 206 bits (525), Expect = 2e-51, Method: Compositional matrix adjust.
Identities = 133/241 (56%), Positives = 148/241 (62%), Gaps = 17/241 (7%)
Query 15 ARFAVGRPVRAVWVNELGGVTFRVDSGMGAGCE---FIKVARRGTA-DFANEARRLRWAA 70
A A GR VW NELGG+TFR+D G E F+K A G+A A EA RLRWAA
Sbjct 18 AALARGRRPVPVWRNELGGLTFRLDGDGRPGGEDPLFVKWAPSGSALPLAAEAERLRWAA 77
Query 71 PYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGLRTLHDSLPVH 130
PY VP V G D D +WL T ALP SAV PRW+A P AV ALG GLR LHD LPV
Sbjct 78 PYTPVPEVREYGSDADGSWLATRALPAQSAVAPRWKADPATAVRALGTGLRALHDRLPVA 137
Query 131 SCPFDWSTASRLAKLAPARRAEL------GDSPPVDRLVVCHGDACSPNTILDDTGRCCG 184
CPF WS A RL ARRA + D PP D LVVCHGD C+PNT+L + G
Sbjct 138 ECPFTWSVAHRLEV---ARRAGIVVPADLPDPPPADLLVVCHGDPCAPNTLLHEDGSWAA 194
Query 185 HVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVRDDEFFAAYGVAPDPARIDYYRRLWQ 244
HVD G LGVADRWADLAV T ++ WN+ PG R AYG+APDP R D+YRRLW
Sbjct 195 HVDMGALGVADRWADLAVVTRNIDWNY--GPGWER--TLLDAYGIAPDPVRTDFYRRLWD 250
Query 245 A 245
A
Sbjct 251 A 251
>gi|294629440|ref|ZP_06708000.1| kanamycin kinase [Streptomyces sp. e14]
gi|292832773|gb|EFF91122.1| kanamycin kinase [Streptomyces sp. e14]
Length=243
Score = 203 bits (517), Expect = 2e-50, Method: Compositional matrix adjust.
Identities = 124/249 (50%), Positives = 148/249 (60%), Gaps = 30/249 (12%)
Query 23 VRAVWVNELGGVTFRVDSGMGAGCEFIKVARRGT-ADFANEARRLRWAAPYLAVPRVLGV 81
++AVW NELGG+TF+V G+G +F+K G+ D + E RLRWAA + VPRVLG
Sbjct 1 MKAVWENELGGLTFQV--GLGDARQFVKWTPHGSRIDLSAEVARLRWAAGFTTVPRVLGE 58
Query 82 GVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGLRTLHDSLPVHSCPFDWSTASR 141
G D +W+ T LPG SAV + P AV A+GAGLR LHD+LPV CPFDWS R
Sbjct 59 GADASGSWIVTAGLPGRSAVDDHSKRDPATAVRAVGAGLRALHDALPVADCPFDWSAERR 118
Query 142 LAK---------LAPARRAE--------------LGDSPPVDRLVVCHGDACSPNTILDD 178
LA + PA E L D PPVD LVVCHGDAC+PNT++ D
Sbjct 119 LAAVRARAAAGRIDPADWDEDVRHHGTVQRALAVLSDIPPVDELVVCHGDACAPNTLVGD 178
Query 179 TGRCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVRDDEFFAAYGVAPDPARIDY 238
G C GHVD G+LGVADRWADLAVAT S +WN+ G + AYGV D RI Y
Sbjct 179 DGACTGHVDLGSLGVADRWADLAVATWSTRWNY----GPGWEKPLLDAYGVTADRERIAY 234
Query 239 YRRLWQAED 247
YR LW+ D
Sbjct 235 YRLLWELSD 243
>gi|336320062|ref|YP_004600030.1| aminoglycoside phosphotransferase [Cellvibrio gilvus ATCC 13127]
gi|336103643|gb|AEI11462.1| aminoglycoside phosphotransferase [Cellvibrio gilvus ATCC 13127]
Length=240
Score = 195 bits (495), Expect = 7e-48, Method: Compositional matrix adjust.
Identities = 122/247 (50%), Positives = 147/247 (60%), Gaps = 12/247 (4%)
Query 1 VSFPSSPPALPAIVARFAVGRPVRAVWVNELGGVTFRV-DSGMGAGCEFIKVARRGT-AD 58
+S P+ +P + R P+ VW N +GG+TF D G+ + K A GT D
Sbjct 1 MSIPTGTVEVPGAIRRLLGDGPLHPVWQNLVGGLTFHAPDRGL-----YAKWAPAGTEGD 55
Query 59 FANEARRLRWAAPYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGA 118
A+EA RL W A + VP V+ G D + WL T A+ G SAVHP+W A P+VAV A+GA
Sbjct 56 LADEAERLAWVARWTPVPHVVETGADDEGGWLVTRAIEGRSAVHPQWVAHPEVAVRAVGA 115
Query 119 GLRTLHDSLPVHSCPFDWSTA-SRLAKLAPARRAELGDSPPVDRLVVCHGDACSPNTILD 177
GLR LHD+LPV CP+ WS A A LG PPVDRLVVCHGDAC PNT+L
Sbjct 116 GLRALHDALPVAGCPWTWSARDRLARVADRAAAAALGAPPPVDRLVVCHGDACVPNTLLS 175
Query 178 DTGRCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVRDDEFFAAYGVAPDPARID 237
D G HVD G+LGVADRWADLAVAT S WN+ G ++ AYG+APDP R
Sbjct 176 DDGTWLAHVDLGSLGVADRWADLAVATWSTVWNY----GPGHEEALLDAYGIAPDPVRTA 231
Query 238 YYRRLWQ 244
YYR LW
Sbjct 232 YYRTLWS 238
>gi|256395310|ref|YP_003116874.1| aminoglycoside phosphotransferase [Catenulispora acidiphila DSM
44928]
gi|256361536|gb|ACU75033.1| aminoglycoside phosphotransferase [Catenulispora acidiphila DSM
44928]
Length=267
Score = 190 bits (483), Expect = 1e-46, Method: Compositional matrix adjust.
Identities = 117/270 (44%), Positives = 149/270 (56%), Gaps = 35/270 (12%)
Query 1 VSFPSSPPA---LPAIVARFAVGRPVRAVWVNELGGVTFRVDSGMGAGCEFIKVARRGT- 56
+SF + PPA +P V A GR + VW NE GG TFR+ G + K A G+
Sbjct 4 LSFNNIPPADHPIPDAVRDAAGGRTIVPVWFNE-GGSTFRL--GDAPDARYAKWAPAGSY 60
Query 57 ADFANEARRLRWAAPYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPAL 116
D A EA R+RWA ++ VP+V+ + + L T AL G AV RW+ P AV +
Sbjct 61 LDLAGEAARIRWAGAFVTVPQVVEQAASAEGSLLVTAALEGEMAVTERWKQDPATAVRVI 120
Query 117 GAGLRTLHDSLPVHSCPFDWSTASRLAKLAPARRAE----------------------LG 154
G LR HD+LPV SCPF +S R+A+ R AE +
Sbjct 121 GESLRAFHDALPVDSCPFSFSAELRVAE--AVRYAEELKDRTMHPDFAGLTREQALKRVA 178
Query 155 DSPPVDRLVVCHGDACSPNTILDDTGRCCGHVDFGNLGVADRWADLAVATLSLQWNFPDY 214
D PPVD+LVVCHGD C+PNT+L GR GHVD G+LGVADRW+DLA+AT S WN+
Sbjct 179 DIPPVDKLVVCHGDTCAPNTLLSADGRFAGHVDLGDLGVADRWSDLAIATWSTTWNY--- 235
Query 215 PGQVRDDEFFAAYGVAPDPARIDYYRRLWQ 244
G + AYG+APDP R +YYR LW+
Sbjct 236 -GDGWQEALLEAYGIAPDPERTEYYRLLWE 264
>gi|296129035|ref|YP_003636285.1| aminoglycoside phosphotransferase [Cellulomonas flavigena DSM
20109]
gi|296020850|gb|ADG74086.1| aminoglycoside phosphotransferase [Cellulomonas flavigena DSM
20109]
Length=282
Score = 180 bits (457), Expect = 1e-43, Method: Compositional matrix adjust.
Identities = 125/267 (47%), Positives = 147/267 (56%), Gaps = 34/267 (12%)
Query 3 FPSSPPALPAIVARFAVGRPVRAVWVNELGGVTFRVDSGMGAGCEFIKVARRGTA--DFA 60
P+ +PA VA A G+ V VWVNE GG TFR+ F+K A R D
Sbjct 25 VPTGTVPVPASVAALAGGQDVEPVWVNEAGGRTFRLGRE-----RFVKWAPRHVPELDLP 79
Query 61 NEARRLRWAAPYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGL 120
EA RL WA + VP VL +GVD + +WL T A+P SAV P W A P A A+GAGL
Sbjct 80 AEALRLTWAGRFTPVPEVLDLGVDDEGSWLVTAAIPARSAVDPMWLARPAEAAAAVGAGL 139
Query 121 RTLHDSLPVHSCPFDWSTASRLAKL---------------------APARRAELGDSPPV 159
R LHD+LP CPF W +R+ + A RA L D+PPV
Sbjct 140 RALHDALPAGECPFAWQVEARVERALEHLHAGDSPTGWDPVHHHLSAADARARLLDAPPV 199
Query 160 DRLVVCHGDACSPNTILDDTGRCCGHVDFGNLGVADRWADLAVATLSLQWNF-PDYPGQV 218
DRLVVCH DAC+PNT++ D GR HVD G LGVADRWADLA+AT S WN+ P Y V
Sbjct 200 DRLVVCHADACAPNTLVHDDGRWAAHVDLGRLGVADRWADLAIATWSTTWNYGPGYERHV 259
Query 219 RDDEFFAAYGVAPDPARIDYYRRLWQA 245
D AYG+ PD R YYR LW A
Sbjct 260 LD-----AYGIEPDDERTAYYRLLWDA 281
>gi|308176109|ref|YP_003915515.1| phosphotransferase [Arthrobacter arilaitensis Re117]
gi|307743572|emb|CBT74544.1| putative phosphotransferase [Arthrobacter arilaitensis Re117]
Length=246
Score = 169 bits (429), Expect = 3e-40, Method: Compositional matrix adjust.
Identities = 96/229 (42%), Positives = 123/229 (54%), Gaps = 10/229 (4%)
Query 21 RPVRAVWVNELGGVTFRVDSGMGAGCEFIK----VARRGTADFANEARRLRWAAPYLAVP 76
+ +W N LGGVT+R+ G G F K D EA+R+ WA ++ VP
Sbjct 23 EEIEGIWQNALGGVTYRI--GNGERTRFAKWSPGCPPEEECDLTAEAQRMSWAGEFIRVP 80
Query 77 RVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGLRTLHDSLPVHSCPFDW 136
VL D L T AL G SA ++ P + A+G GLR +HD+LPV +CPFDW
Sbjct 81 EVLSCEDHADGQLLVTAALHGHSAASSLGKSDPARSARAVGQGLRQMHDALPVGACPFDW 140
Query 137 STASRLAKLAPARRAELGDSPPVDRLVVCHGDACSPNTILDDTGRCCGHVDFGNLGVADR 196
SR+ L +RAEL P LVVCHGDAC PNTI+ D HVD G LG+ADR
Sbjct 141 DLESRIHHLPAGQRAELLAEAPELDLVVCHGDACLPNTIITDDHALSAHVDMGKLGLADR 200
Query 197 WADLAVATLSLQWNFPDYPGQVRDDEFFAAYGVAPDPARIDYYRRLWQA 245
WADLA+A S +WN+ G + +A Y PD +I +YR +W A
Sbjct 201 WADLAIAAWSTEWNY----GPGFEQYVYAGYCTEPDERKIAFYRGVWDA 245
>gi|184200695|ref|YP_001854902.1| hypothetical protein KRH_10490 [Kocuria rhizophila DC2201]
gi|183580925|dbj|BAG29396.1| hypothetical protein [Kocuria rhizophila DC2201]
Length=236
Score = 149 bits (375), Expect = 5e-34, Method: Compositional matrix adjust.
Identities = 103/232 (45%), Positives = 130/232 (57%), Gaps = 18/232 (7%)
Query 3 FPSSPPALPAIVAR----FAVGRP-VRAVWVNELGGVTFRVDSGMGAGC---EFIKVARR 54
P PALP ++ R F + AVW N +GG+TF V SG G G ++ +
Sbjct 10 LPPVNPALPDLLRRALAAFGLSETGASAVWQNGVGGLTFAVASG-GPGSPADDYAQWNPS 68
Query 55 GTAD-FANEARRLRWAAPYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPR-WRASPQVA 112
G+ + A+EA R+RW P V+ + DG L T ALPG SAV R WR P A
Sbjct 69 GSGESLADEAERMRWIQGRHPAPTVVELVADGGEEVLLTQALPGESAVSARRWREHPDTA 128
Query 113 VPALGAGLRTLHDSLPVHSCPFDWSTASRLAKLAPARRAELGDSPPVDRLVVCHGDACSP 172
+ ALG GLR LHD + V CPF W+ SR+ +LA A LGD+P VDRLV+C GD C+P
Sbjct 129 LQALGTGLRELHD-VAVDDCPFAWNVDSRI-RLAGADARVLGDAPDVDRLVLCQGDPCAP 186
Query 173 NTILDDTGRCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVRDDEFF 224
NT+ G HVD G LGVADRWADL+V ++SL W+ Y DD F
Sbjct 187 NTLPARDGSFLAHVDLGRLGVADRWADLSVMSMSLAWSCRAY-----DDSVF 233
>gi|297565411|ref|YP_003684383.1| aminoglycoside phosphotransferase [Meiothermus silvanus DSM 9946]
gi|296849860|gb|ADH62875.1| aminoglycoside phosphotransferase [Meiothermus silvanus DSM 9946]
Length=270
Score = 100 bits (249), Expect = 2e-19, Method: Compositional matrix adjust.
Identities = 92/269 (35%), Positives = 119/269 (45%), Gaps = 44/269 (16%)
Query 9 ALPAIVARFAVGRPVRAVWVNELGGVTFRVDSGMGAGCEFIKVARRGTAD------FANE 62
ALP V+R+ G V V G +R G I R + D A E
Sbjct 7 ALPQAVSRWLQGYEAEPVTVGMSGAGVYRWRKAGKPG--LILKIRPDSHDPDPGFRLAPE 64
Query 63 ARRLRWAAPYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRW--RASPQVAVPALGAGL 120
A ++RW + ++ P VL +L + G S W P+V V A+ L
Sbjct 65 AEKMRWLSAWVPGPEVLEYSQQDGCEYLLMTEIVGRSGAE-NWPPEERPRVVV-AIAEAL 122
Query 121 RTLHDSLPVHSCPFDWSTASRLAKLAPAR-RAELG------------------------D 155
R LH S+P+ CPFD AKLA AR R ELG
Sbjct 123 RKLH-SIPLERCPFDQRLE---AKLAQARQRTELGLVDLADFDERWQGKSAEEPLEVLLQ 178
Query 156 SPPVDR-LVVCHGDACSPNTILDDTGRCCGHVDFGNLGVADRWADLAVATLSLQWNFPDY 214
+ P D LVVCHGD C PN +L + G+ G VD G LGVADR+ DLA+ T SL +
Sbjct 179 TRPTDEDLVVCHGDYCLPNVLLQE-GKLSGFVDLGRLGVADRYQDLALMTRSLTSDLNPQ 237
Query 215 PGQVRDDEFFAAYGV-APDPARIDYYRRL 242
G+ D F AYG+ PD R+++YR L
Sbjct 238 FGEGWDRVFLEAYGIREPDWTRLEFYRLL 266
>gi|327189670|gb|EGE56818.1| putative aminoglycoside 3'-phosphotransferase protein [Rhizobium
etli CNPAF512]
Length=255
Score = 99.8 bits (247), Expect = 3e-19, Method: Compositional matrix adjust.
Identities = 86/234 (37%), Positives = 114/234 (49%), Gaps = 34/234 (14%)
Query 35 TFRVDSGMGAGCEFIKVARRG-TADFANEARRLRW-AAPYLAVPRVLGVGVDGDWAWLHT 92
FR++ G G ++KV G + A+EA RL W A L P V+ DG+ WL
Sbjct 26 VFRLE-GEGLPALYLKVEAAGPFGELADEAARLSWLKAVGLPCPDVIARESDGERNWLLI 84
Query 93 DALPGLSAVHPRWRASPQVAVPALGAGLRTLHDSLPVHSCPFDWST-------------- 138
+ALPG + + +P V L A L LHD LP+ SCPFD
Sbjct 85 NALPG-TDLASASALTPLTRVELLAAALSDLHD-LPIASCPFDHRLENRLPTAKARMQAG 142
Query 139 --------ASRLAKLAPARRAELGDSPPVDR-LVVCHGDACSPNTILDDTGRCCGHVDFG 189
A+RL K A A EL P D LVV HGDAC PN + + G+ G++D
Sbjct 143 IVDEEDFDAARLGKSAAALFIELMRLRPGDEELVVTHGDACLPNFVASN-GQFSGYIDCS 201
Query 190 NLGVADRWADLAVATLSLQWNFPDYPGQVRDDEFFAAYGV-APDPARIDYYRRL 242
LGVADR D+A+A S+ NF G+ F YG+ APDPA++ Y++ L
Sbjct 202 RLGVADRHQDIALACRSIASNF----GEALIRPFLDRYGLTAPDPAKLHYFQLL 251
>gi|194365494|ref|YP_002028104.1| aminoglycoside phosphotransferase [Stenotrophomonas maltophilia
R551-3]
gi|194348298|gb|ACF51421.1| aminoglycoside phosphotransferase [Stenotrophomonas maltophilia
R551-3]
Length=267
Score = 99.0 bits (245), Expect = 5e-19, Method: Compositional matrix adjust.
Identities = 92/261 (36%), Positives = 111/261 (43%), Gaps = 45/261 (17%)
Query 20 GRPVRAVWVNELGGVTFRVDSGMGAGCEFIKVARRGTAD-------------FANEARRL 66
G P+ + W L G S + + +V R G AD +E RL
Sbjct 10 GMPLPSAWREALAGARIERQSIGVSRADVARVHRPGQADAFLKSEVIDPFSELGDEIARL 69
Query 67 RW-AAPYLAVPRVLGVGVDGDWAWLHTDALPGLS-AVHPRWRASPQVAVPALGAGLRTLH 124
RW A VP V+ D WL ALPG A P PQ V L LR LH
Sbjct 70 RWLQAQGQPVPTVIATAEDAGRRWLLMSALPGRDLASSPEL--VPQRLVEVLADALRGLH 127
Query 125 DSLPVHSCPFDWSTASR----------------------LAKLAPARRAELGDSPPV-DR 161
+LPV +CPFD ASR L + A AEL S P +
Sbjct 128 -ALPVAACPFDQRLASRVQAAVARAEAGLVDADDFDDERLGQSAQQVLAELLASQPAHED 186
Query 162 LVVCHGDACSPNTILDDTGRCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVRDD 221
LVV HGDAC PN ++ + GR G +D G LGVADR+ DLA+A SL NF D
Sbjct 187 LVVSHGDACLPNLMVAE-GRFSGFIDCGRLGVADRYQDLALAARSLVHNFGDTRWVA--- 242
Query 222 EFFAAYGVAPDPARIDYYRRL 242
F YG D R+ +YR L
Sbjct 243 PLFQRYGAVADERRLAFYRLL 263
>gi|241205653|ref|YP_002976749.1| aminoglycoside phosphotransferase [Rhizobium leguminosarum bv.
trifolii WSM1325]
gi|240859543|gb|ACS57210.1| aminoglycoside phosphotransferase [Rhizobium leguminosarum bv.
trifolii WSM1325]
Length=265
Score = 96.7 bits (239), Expect = 3e-18, Method: Compositional matrix adjust.
Identities = 92/263 (35%), Positives = 121/263 (47%), Gaps = 35/263 (13%)
Query 6 SPPALPAIVARFAVGRPVRAVWVNELGGVTFRVDSGMGAGCEFIKVARRG-TADFANEAR 64
PP+ A+ AR A R R V FR+++ +G ++KV + G + A+EA
Sbjct 8 QPPSAGALDARLAGYRFERDALGRSAASV-FRLEA-LGLPTLYLKVEQAGPFGELADEAA 65
Query 65 RLRWAAPY-LAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGLRTL 123
RLRW L P V+ DG L ALPG S + +P V L L L
Sbjct 66 RLRWLQTSGLPCPDVIAEDSDGVHNRLLISALPG-SDLTSASALTPPARVELLAIALLDL 124
Query 124 HDSLPVHSCPFDWSTASRLA----------------------KLAPARRAELGDSPPV-D 160
H LP+ SCPFD R+A K A A AEL P +
Sbjct 125 H-RLPIASCPFDHRLERRVAAAKARMQAGIVDETDFDETRVGKSAEALFAELESGKPSRE 183
Query 161 RLVVCHGDACSPNTILDDTGRCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVRD 220
LVV HGDAC PN + + G G++D LGVADR+ D+A+A S+ NF G+
Sbjct 184 DLVVIHGDACLPNFVASEEG-FSGYIDCSRLGVADRYQDIALACRSIAHNF----GEALV 238
Query 221 DEFFAAYGV-APDPARIDYYRRL 242
F YG+ A DPAR+ YY+ L
Sbjct 239 QPFLDRYGMPATDPARLAYYQLL 261
>gi|344207168|ref|YP_004792309.1| Kanamycin kinase [Burkholderia sp. JV3]
gi|343778530|gb|AEM51083.1| Kanamycin kinase [Burkholderia sp. JV3]
Length=267
Score = 95.9 bits (237), Expect = 5e-18, Method: Compositional matrix adjust.
Identities = 92/261 (36%), Positives = 110/261 (43%), Gaps = 45/261 (17%)
Query 20 GRPVRAVWVNELGGVTFRVDSGMGAGCEFIKVARRGTAD-------------FANEARRL 66
G P+ W L S + + +V R G AD +E RL
Sbjct 10 GMPLPHAWQEALADARIERQSIGVSRADVARVHRPGQADAFLKSEVIDAFSELGDEIERL 69
Query 67 RW-AAPYLAVPRVLGVGVDGDWAWLHTDALPGLS-AVHPRWRASPQVAVPALGAGLRTLH 124
RW A P V+ + WL ALPG A PR +P V L LR LH
Sbjct 70 RWLQAQGQPAPMVIATAEEAGRRWLLMSALPGHDLASSPRL--APHHLVELLADALRGLH 127
Query 125 DSLPVHSCPFDWSTASRL----AKLAPAR-------RAELGDS------------PPVDR 161
+LPV SCPFD ASRL A++ R LG S P +
Sbjct 128 -ALPVASCPFDQRLASRLQAAQARVEAGRVDADDFDDERLGQSAQQVFAELRTTRPDHED 186
Query 162 LVVCHGDACSPNTILDDTGRCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVRDD 221
LVV HGDAC PN +L GR G +D G LGVADR+ DLA+A SL NF D
Sbjct 187 LVVSHGDACLPN-LLAAEGRFSGFIDCGRLGVADRYQDLALAARSLVHNFGDTRWVA--- 242
Query 222 EFFAAYGVAPDPARIDYYRRL 242
F YGV D R+ +YR L
Sbjct 243 PLFRRYGVVADERRLAFYRLL 263
>gi|190892695|ref|YP_001979237.1| aminoglycoside 3'-phosphotransferase [Rhizobium etli CIAT 652]
gi|190697974|gb|ACE92059.1| putative aminoglycoside 3'-phosphotransferase protein [Rhizobium
etli CIAT 652]
Length=261
Score = 95.1 bits (235), Expect = 9e-18, Method: Compositional matrix adjust.
Identities = 84/235 (36%), Positives = 111/235 (48%), Gaps = 36/235 (15%)
Query 35 TFRVDSGMGAGCEFIKVARRG-TADFANEARRLRW-AAPYLAVPRVLGVGVDGDWAWLHT 92
FR++ G G ++KV G + A+EA RL W A L P V+ DG+ WL
Sbjct 32 VFRLE-GEGLPALYLKVEAAGPFGELADEAARLSWLKAAGLPCPDVIARESDGERNWLLI 90
Query 93 DALPGLSAVHPRWRASPQVAVPALGAGLRTLHDSLPVHSCPFDWSTASRLAKLAPARR-- 150
ALPG V +P V L A L LHD LP+ SCPFD +RL A AR
Sbjct 91 GALPGADLVSASA-LTPMARVELLAAALSDLHD-LPIASCPFDHRLENRLPT-AKARMEA 147
Query 151 ----------AELGDS------------PPVDRLVVCHGDACSPNTILDDTGRCCGHVDF 188
A LG + P + LVV HGDAC PN + + G+ G++D
Sbjct 148 GIVDEEDFDAARLGKNAAALFDDLMRLRPGNEDLVVTHGDACLPNFVASN-GKFSGYIDC 206
Query 189 GNLGVADRWADLAVATLSLQWNFPDYPGQVRDDEFFAAYGV-APDPARIDYYRRL 242
LGVADR D+A+A S+ NF G+ F YG+ APD ++ Y++ L
Sbjct 207 SRLGVADRHQDIALACRSIASNF----GEALIRPFLERYGLTAPDATKLHYFQLL 257
>gi|297154663|gb|ADI04375.1| phosphotransferase [Streptomyces bingchenggensis BCW-1]
Length=157
Score = 94.4 bits (233), Expect = 1e-17, Method: Compositional matrix adjust.
Identities = 51/89 (58%), Positives = 58/89 (66%), Gaps = 9/89 (10%)
Query 119 GLRTLHDSLPVHSCPFDWSTASRLAKLAPARRA------ELGDSPPVDRLVVCHGDACSP 172
LR LH+ LPV CPF WS RL ARRA +L ++PPVD LVVCHGD C+P
Sbjct 65 ALRALHEQLPVADCPFSWSVDHRLGI---ARRAGIEVPADLPEAPPVDHLVVCHGDPCAP 121
Query 173 NTILDDTGRCCGHVDFGNLGVADRWADLA 201
NT+L + G HVD G LGVADRWADLA
Sbjct 122 NTLLPEEGSWSAHVDLGALGVADRWADLA 150
>gi|254524099|ref|ZP_05136154.1| aminoglycoside phosphotransferase [Stenotrophomonas sp. SKA14]
gi|219721690|gb|EED40215.1| aminoglycoside phosphotransferase [Stenotrophomonas sp. SKA14]
Length=329
Score = 94.4 bits (233), Expect = 1e-17, Method: Compositional matrix adjust.
Identities = 88/254 (35%), Positives = 107/254 (43%), Gaps = 45/254 (17%)
Query 27 WVNELGGVTFRVDSGMGAGCEFIKVARRGTAD-------------FANEARRLRW-AAPY 72
W +L F S + + +V R G AD +E RLRW A
Sbjct 79 WQQDLADARFERQSIGVSRADVARVHRPGQADAFLKSEVIDAFSELGDEIARLRWLQAQG 138
Query 73 LAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGLRTLHDSLPVHSC 132
P V+ + WL ALPG + PQ + L LR LH +LPV +C
Sbjct 139 QPAPTVMATAEEAGRRWLLMSALPGRD-LATSVDLPPQQVLAVLADALRGLH-ALPVAAC 196
Query 133 PFDWSTASRLAKLAPAR------------RAELGDS------------PPVDRLVVCHGD 168
PFD ASRL A AR LG S P + VVCHGD
Sbjct 197 PFDQRLASRL-HAAQARVEAGVVDADDFDDERLGQSAQQVFVELCASRPAHEDRVVCHGD 255
Query 169 ACSPNTILDDTGRCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVRDDEFFAAYG 228
AC PN ++ D GR G +D G LGVADR+ DLA+A SL NF D F YG
Sbjct 256 ACLPNLMVAD-GRFSGFIDCGRLGVADRYQDLALAARSLVHNFGDTRWVA---PLFQRYG 311
Query 229 VAPDPARIDYYRRL 242
V D R+ +YR L
Sbjct 312 VIADERRLAFYRLL 325
>gi|312191071|gb|ADQ43421.1| aminoglycoside 3'-phosphotransferase [Stenotrophomonas maltophilia]
Length=267
Score = 94.4 bits (233), Expect = 1e-17, Method: Compositional matrix adjust.
Identities = 80/213 (38%), Positives = 100/213 (47%), Gaps = 36/213 (16%)
Query 57 ADFANEARRLRW-AAPYLAVPRVLGVGVDGDWAWLHTDALPGLS-AVHPRWRASPQVAVP 114
++ +E RLRW A + P V+ +G WL ALPG A P +P+
Sbjct 60 SELGDEIARLRWLQAQGQSAPTVIATTEEGGRRWLLMSALPGRDLASSPEL--APRRVAE 117
Query 115 ALGAGLRTLHDSLPVHSCPFDWSTASRLAKLAPAR------------RAELGDSPPV--- 159
L LR LH ++PV +CPFD ASRL + A AR LG SP
Sbjct 118 LLADALRGLH-AVPVANCPFDQQLASRL-QAAQARVEAGLVDADDFDDERLGQSPQQVFA 175
Query 160 ---------DRLVVCHGDACSPNTILDDTGRCCGHVDFGNLGVADRWADLAVATLSLQWN 210
+ LVV GDAC PN + D GR G +D G LGVADR+ DLA+A SL N
Sbjct 176 ELRATRPAHEDLVVSQGDACLPNLTVTD-GRFTGFIDCGRLGVADRYQDLALAARSLVHN 234
Query 211 FPDYPGQVR-DDEFFAAYGVAPDPARIDYYRRL 242
F G+ R F YG PD R+ +YR L
Sbjct 235 F----GESRCVAALFQRYGAVPDERRLAFYRLL 263
>gi|86358549|ref|YP_470441.1| aminoglycoside 3`-phosphotransferase [Rhizobium etli CFN 42]
gi|86282651|gb|ABC91714.1| putative aminoglycoside 3`-phosphotransferase protein [Rhizobium
etli CFN 42]
Length=261
Score = 94.0 bits (232), Expect = 2e-17, Method: Compositional matrix adjust.
Identities = 93/264 (36%), Positives = 118/264 (45%), Gaps = 36/264 (13%)
Query 6 SPPALPAIVARFAVGRPVRAVWVNELGGVTFRVDSGMGAGCEFIKVARRG-TADFANEAR 64
SP ALP + G + FR+ G G ++KV G + A+EA
Sbjct 3 SPQALPGALGERLSGYRFERDALGRSAASVFRL-KGEGLPALYLKVEAAGPFGELADEAA 61
Query 65 RLRWA-APYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGLRTL 123
RL W A L P V+ DG+ WL ALPG A V L L L
Sbjct 62 RLGWLNASSLPCPEVIARESDGERNWLLVSALPGTDLASASALAP-LARVELLAEALLDL 120
Query 124 HDSLPVHSCPFDWSTASRLAKLAPARR------------AELGDS------------PPV 159
H LP+ SCPFD RL +A AR A LG S P
Sbjct 121 H-RLPITSCPFDHRLEKRL-PVAKARMDGGIVDEEDFDPARLGKSAAELFAALESMRPTD 178
Query 160 DRLVVCHGDACSPNTILDDTGRCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVR 219
+ LVV HGDAC PN ++ G+ G++D LGVADR+ D+A+A S+ NF G+
Sbjct 179 EDLVVTHGDACLPNFVVS-GGQFSGYIDCSRLGVADRYQDIALACRSITDNF----GEAL 233
Query 220 DDEFFAAYGV-APDPARIDYYRRL 242
F YG+ APDPAR+DYY+ L
Sbjct 234 VQPFLHRYGLPAPDPARLDYYQLL 257
>gi|226357205|ref|YP_002786945.1| kanamycin kinase (aminoglycoside 3-phosphotransferase, neomycin-kanamycin
phosphotransferase) [Deinococcus deserti VCD115]
Length=264
Score = 92.8 bits (229), Expect = 4e-17, Method: Compositional matrix adjust.
Identities = 77/220 (35%), Positives = 98/220 (45%), Gaps = 37/220 (16%)
Query 49 IKVARRGTA---DFANEARRLRWAAPYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRW 105
+KV R A E RLR+ A + VPRV+G GD +L +PG+ P
Sbjct 46 VKVQTRSAAPVSTLQQERERLRFFAGRVPVPRVIGYETHGDTEYLAMTRVPGIPMSDPDA 105
Query 106 RASPQVAVPALGAGLRTLHDSLPVHSCPFDWSTASRLAKLAPARRAELG-------DS-- 156
P+ V L LR LH +LP+ CPF+ + A L R E G DS
Sbjct 106 TLHPERVVTLLARALRELH-ALPLRDCPFNMTLAYTLH--VARERVEAGVVDEADFDSHR 162
Query 157 ----------------PPVDRLVVCHGDACSPNTILDDTGRCCGHVDFGNLGVADRWADL 200
P + LVV HGDAC PN I+ G VD G G+ADR ADL
Sbjct 163 QGRSAVSVFNELVRCRPETEDLVVTHGDACLPNFIISGEF-VEGLVDVGRAGLADRHADL 221
Query 201 AVATLSLQWNFPDYPGQVRDDEFFAAYGVA-PDPARIDYY 239
A+A S+Q+N G+ F YG A D +R+DYY
Sbjct 222 ALAWRSVQYNL----GETYAGMFLDLYGPALVDQSRLDYY 257
>gi|315271315|gb|ACO47191.2| putative kanamycin kinase (aminoglycoside 3-phosphotransferase)
(neomycin-kanamycin phosphotransferase) [Deinococcus deserti
VCD115]
Length=260
Score = 92.4 bits (228), Expect = 5e-17, Method: Compositional matrix adjust.
Identities = 78/221 (36%), Positives = 100/221 (46%), Gaps = 39/221 (17%)
Query 49 IKVARRGTA---DFANEARRLRWAAPYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRW 105
+KV R A E RLR+ A + VPRV+G GD +L +PG+ P
Sbjct 42 VKVQTRSAAPVSTLQQERERLRFFAGRVPVPRVIGYETHGDTEYLAMTRVPGIPMSDPDA 101
Query 106 RASPQVAVPALGAGLRTLHDSLPVHSCPFDWSTASRLAKLAPARRAELG-------DS-- 156
P+ V L LR LH +LP+ CPF+ + A L R E G DS
Sbjct 102 TLHPERVVTLLARALRELH-ALPLRDCPFNMTLAYTLH--VARERVEAGVVDEADFDSHR 158
Query 157 ----------------PPVDRLVVCHGDACSPNTILDDTGRCC-GHVDFGNLGVADRWAD 199
P + LVV HGDAC PN I+ +G G VD G G+ADR AD
Sbjct 159 QGRSAVSVFNELVRCRPETEDLVVTHGDACLPNFII--SGEFVEGLVDVGRAGLADRHAD 216
Query 200 LAVATLSLQWNFPDYPGQVRDDEFFAAYGVA-PDPARIDYY 239
LA+A S+Q+N G+ F YG A D +R+DYY
Sbjct 217 LALAWRSVQYNL----GETYAGMFLDLYGPALVDQSRLDYY 253
>gi|62857277|dbj|BAD95814.1| aminoglycoside 3'-phosphotransferase [Streptomyces fradiae]
gi|66947462|emb|CAH58684.1| putative neomycin phosphotransferase [Streptomyces fradiae]
gi|71360898|emb|CAH05108.1| aminoglycoside phosphotransferase [Streptomyces fradiae]
gi|85813562|emb|CAF33306.1| aminoglycoside 3'-phosphotransferase [Streptomyces fradiae]
Length=268
Score = 91.3 bits (225), Expect = 1e-16, Method: Compositional matrix adjust.
Identities = 75/213 (36%), Positives = 102/213 (48%), Gaps = 31/213 (14%)
Query 58 DFANEARRLRWAAPY-LAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAV-PA 115
D + EA RL W + + VPRV+ G D AWL T+A+PG++A W + AV A
Sbjct 55 DLSGEADRLEWLHRHGIPVPRVVERGADDTAAWLVTEAVPGVAAAE-EWPEHQRFAVVEA 113
Query 116 LGAGLRTLHDSLPVHSCPFDWSTASRLAK--------------LAPARRAELGDS----- 156
+ R LH+ LPV CPFD + +A+ L R GD
Sbjct 114 MAELARALHE-LPVEDCPFDRRLDAAVAEARRNVAEGLVDLDDLQEERAGWTGDQLLAEL 172
Query 157 ----PPVDRLVVCHGDACSPNTILDDTGRC--CGHVDFGNLGVADRWADLAVATLSLQWN 210
P + LVVCHGD C PN +L D G C G +D G LGVADR AD+A+A L+ +
Sbjct 173 DRTRPEKEDLVVCHGDLC-PNNVLLDPGTCRVTGVIDVGRLGVADRHADIALAARELEID 231
Query 211 FPDYPGQVRDDEFFAAYGVAP-DPARIDYYRRL 242
+ G + F YG D ++ +Y+ L
Sbjct 232 EDPWFGPAYAERFLERYGAHRVDKEKLAFYQLL 264
>gi|15805107|ref|NP_293792.1| aminoglycoside 3`-phosphotransferase [Deinococcus radiodurans
R1]
gi|6457725|gb|AAF09655.1|AE001869_4 aminoglycoside 3`-phosphotransferase [Deinococcus radiodurans
R1]
Length=263
Score = 90.9 bits (224), Expect = 2e-16, Method: Compositional matrix adjust.
Identities = 74/212 (35%), Positives = 96/212 (46%), Gaps = 34/212 (16%)
Query 57 ADFANEARRLRWAAPYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPAL 116
+ E RLRW A L VP+V+ DG+ +L LPG++ HP P+ V L
Sbjct 56 STLLQERERLRWFAGRLPVPQVVAYEDDGEQEYLAMTRLPGIAMSHPDAALHPERMVNLL 115
Query 117 GAGLRTLHDSLPVHSCPFDWSTASRLAKLAPARRA-----------------------EL 153
LR LH +LPV CPF+ S RL KLA R A +L
Sbjct 116 ARALRELH-ALPVRDCPFNMSLGVRL-KLARERVAAGVVDESDFDEERQGQSAVQVFNQL 173
Query 154 GDSPPVDR-LVVCHGDACSPNTILDDTGRCCGHVDFGNLGVADRWADLAVATLSLQWNF- 211
+ P + LVV HGDAC PN I+ G +D G G+ADR DLA+A S + N
Sbjct 174 ARTRPAEEDLVVTHGDACLPNVIVQGE-YVEGLIDLGRAGIADRHMDLALAWRSTRRNLG 232
Query 212 PDYPGQVRDDEFFAAYG-VAPDPARIDYYRRL 242
Y G + D YG D ++ YY+ L
Sbjct 233 ATYAGMLLD-----LYGRELVDEGKLAYYQLL 259
>gi|320332959|ref|YP_004169670.1| aminoglycoside phosphotransferase [Deinococcus maricopensis DSM
21211]
gi|319754248|gb|ADV66005.1| aminoglycoside phosphotransferase [Deinococcus maricopensis DSM
21211]
Length=304
Score = 89.4 bits (220), Expect = 4e-16, Method: Compositional matrix adjust.
Identities = 76/219 (35%), Positives = 99/219 (46%), Gaps = 31/219 (14%)
Query 49 IKVARRGTAD-FANEARRLRWAAPYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRA 107
+K R G D E RL + A + VP V+ V+ D +L + LPG HP +
Sbjct 88 LKRQRSGDFDTLRGERERLGYFAGRVRVPDVVAYHVEDDVEYLVVERLPGTDMSHPALQR 147
Query 108 SPQVAVPALGAGLRTLHDSLPVHSCPFDWSTASRLA-----------------KLAPARR 150
P+ A L LR +H +LPVH CPFD RLA RR
Sbjct 148 DPRRAAELLARALREVH-ALPVHDCPFDRRLRVRLADARERVRRGLVDEDDFDDERRGRR 206
Query 151 A-----ELGDSPPVDR-LVVCHGDACSPNTILDDTGRCCGHVDFGNLGVADRWADLAVAT 204
A ++ S P D LVV HGDA N ++ D G G +D G GVADR DLA+A
Sbjct 207 ATDLLNDVEQSCPADEDLVVTHGDAYVHNVMVAD-GELAGLIDVGRAGVADRHMDLALAA 265
Query 205 LSLQWNFPDYPGQVRDDEFFAAYGVAP-DPARIDYYRRL 242
SL ++ + QV F AYG D ++ +YR L
Sbjct 266 GSLASDYGEGVDQV----FLEAYGREHVDEEKLRFYRLL 300
>gi|78063044|ref|YP_372952.1| aminoglycoside phosphotransferase [Burkholderia sp. 383]
gi|77970929|gb|ABB12308.1| Aminoglycoside phosphotransferase [Burkholderia sp. 383]
Length=264
Score = 89.0 bits (219), Expect = 6e-16, Method: Compositional matrix adjust.
Identities = 88/253 (35%), Positives = 120/253 (48%), Gaps = 53/253 (20%)
Query 27 WVNELGGVT----FRVDSGMGAGCEFIKVARRG-TADFANEARRLRWAAPY-LAVPRVLG 80
W + G + FR+D+ G+ F+K G + ++EA RLRW A L +VL
Sbjct 24 WTQQTEGQSDAAVFRLDARDGSR-RFVKTEPAGPLGELSDEAARLRWLATTGLPGAQVLD 82
Query 81 VGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGLRTLHDSLPVHSCPFDWSTAS 140
V W+ A+ G + R + +VA+ + LR LH P+ +CPFD AS
Sbjct 83 VASAAGREWMLLSAVRGENLEVARLGPAAKVAI--IADALRALHRLDPI-TCPFDHGAAS 139
Query 141 RLAKLAPARRAE-------LGDSP---PVDRL--------------VVCHGDACSPNTIL 176
R+ + A AR E L D+ P+D L VV HGDAC PN ++
Sbjct 140 RIER-ARARMNEGLVDEDNLDDANTGVPLDELFARLQASRPLIEDRVVTHGDACLPNFMV 198
Query 177 DDTGRCCGHVDFGNLGVADRWADLAVAT------LSLQWNFPDYPGQVRDDEFFAAYGVA 230
+D GR G +D G LGVADR+ DLA+AT L +W P FFA YG+
Sbjct 199 ED-GRFSGFIDCGRLGVADRYQDLALATRDIEADLGSEWVAP----------FFARYGIK 247
Query 231 -PDPARIDYYRRL 242
PD RI +YR L
Sbjct 248 QPDAGRIAFYRLL 260
>gi|107103636|ref|ZP_01367554.1| hypothetical protein PaerPA_01004706 [Pseudomonas aeruginosa
PACS2]
Length=261
Score = 88.6 bits (218), Expect = 8e-16, Method: Compositional matrix adjust.
Identities = 85/260 (33%), Positives = 109/260 (42%), Gaps = 51/260 (19%)
Query 25 AVWVNELGGVTFRVDSGMGAGC-----EFIKVARRGT-----------ADFANEARRLRW 68
+ W + L G +R G G GC ++VARR T A+ E RLRW
Sbjct 7 STWADYLAGYRWR---GQGEGCSAATVHRLEVARRPTLFVKQEVLSAHAELPAEIARLRW 63
Query 69 A-APYLAVPRVLGVGVDGDWAWLHTDALPG--LSAVHPRWRASPQVAVPALGAGLRTLHD 125
+ P+VL WL A+PG LSA+ R P+ V + A LR LHD
Sbjct 64 LHGAGIDCPQVLNETQSDGRQWLLMSAVPGDTLSALAQRGELEPERLVRLVAAALRRLHD 123
Query 126 SLPVHSCPFDWSTASRLAKLAPARRAELGDS-----------------------PPVDRL 162
P +CPFD RL + A L D P V+ L
Sbjct 124 LDPA-ACPFDHRLERRLDTVRQRVEAGLVDEADFDDDHRGRSATELYRLLLDRRPAVEDL 182
Query 163 VVCHGDACSPNTILDDTGRCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVRDDE 222
VV HGDAC PN +L + R G +D G LGVADR DLA+A ++ G +
Sbjct 183 VVAHGDACLPN-LLAEGRRFSGFIDCGRLGVADRHQDLALAARDIEAEL----GAAWAEA 237
Query 223 FFAAYGVAPDPARIDYYRRL 242
F YG D R+ Y+R L
Sbjct 238 FLVEYGGDIDGERLAYFRLL 257
>gi|254242804|ref|ZP_04936126.1| aminoglycoside 3'-phosphotransferase type IIb [Pseudomonas aeruginosa
2192]
gi|126196182|gb|EAZ60245.1| aminoglycoside 3'-phosphotransferase type IIb [Pseudomonas aeruginosa
2192]
gi|334840476|gb|EGM19129.1| aminoglycoside phosphotransferase [Pseudomonas aeruginosa 138244]
Length=268
Score = 88.6 bits (218), Expect = 8e-16, Method: Compositional matrix adjust.
Identities = 85/260 (33%), Positives = 109/260 (42%), Gaps = 51/260 (19%)
Query 25 AVWVNELGGVTFRVDSGMGAGC-----EFIKVARRGT-----------ADFANEARRLRW 68
+ W + L G +R G G GC ++VARR T A+ E RLRW
Sbjct 14 STWADYLAGYRWR---GQGEGCSAATVHRLEVARRPTLFVKQEVLSAHAELPAEIARLRW 70
Query 69 A-APYLAVPRVLGVGVDGDWAWLHTDALPG--LSAVHPRWRASPQVAVPALGAGLRTLHD 125
+ P+VL WL A+PG LSA+ R P+ V + A LR LHD
Sbjct 71 LHGAGIDCPQVLNETQSDGRQWLLMSAVPGDTLSALAQRGELEPERLVRLVAAALRRLHD 130
Query 126 SLPVHSCPFDWSTASRLAKLAPARRAELGDS-----------------------PPVDRL 162
P +CPFD RL + A L D P V+ L
Sbjct 131 LDPA-ACPFDHRLERRLDTVRQRVEAGLVDEADFDDDHRGRSATELYRLLLDRRPAVEDL 189
Query 163 VVCHGDACSPNTILDDTGRCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVRDDE 222
VV HGDAC PN +L + R G +D G LGVADR DLA+A ++ G +
Sbjct 190 VVAHGDACLPN-LLAEGRRFSGFIDCGRLGVADRHQDLALAARDIEAEL----GAAWAEA 244
Query 223 FFAAYGVAPDPARIDYYRRL 242
F YG D R+ Y+R L
Sbjct 245 FLVEYGGDIDGERLAYFRLL 264
>gi|222086539|ref|YP_002545073.1| aminoglycoside 3-phosphotransferase protein [Agrobacterium radiobacter
K84]
gi|221723987|gb|ACM27143.1| aminoglycoside 3-phosphotransferase protein [Agrobacterium radiobacter
K84]
Length=267
Score = 88.6 bits (218), Expect = 8e-16, Method: Compositional matrix adjust.
Identities = 74/212 (35%), Positives = 98/212 (47%), Gaps = 34/212 (16%)
Query 57 ADFANEARRLRW-AAPYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPA 115
++ A E RLRW A L P V+ G+ WL +AL G SP V
Sbjct 60 SELAGEVARLRWLGAQGLVCPAVVAHEAGGEREWLLMNALDGADLASASL-ISPLERVRM 118
Query 116 LGAGLRTLHDSLPVHSCPFDWSTASRLAKLAPAR------------RAELGDS------- 156
LR LH + + +CPFD + +R+A A AR A LG S
Sbjct 119 FAEALRDLH-RIRIDACPFDHRSDARIAA-AKARMLAGRVDETDFDEARLGRSAESLFRE 176
Query 157 -----PPVDRLVVCHGDACSPNTILDDTGRCCGHVDFGNLGVADRWADLAVATLSLQWNF 211
P + LVV HGDAC PN I D R G++D LGVADR+ D+A+A S+ +NF
Sbjct 177 LEERRPAAEDLVVTHGDACLPNFIAADH-RFAGYIDCCRLGVADRYQDIALACGSIVYNF 235
Query 212 PDYPGQVRDDEFFAAYGVAP-DPARIDYYRRL 242
G+ F YG++ D ++DYYR L
Sbjct 236 ----GEALVASFLETYGISEVDAEKMDYYRLL 263
>gi|83999850|emb|CAH60150.1| putative phosphotransferase [Streptomyces tenjimariensis]
Length=273
Score = 88.6 bits (218), Expect = 9e-16, Method: Compositional matrix adjust.
Identities = 85/237 (36%), Positives = 106/237 (45%), Gaps = 32/237 (13%)
Query 36 FRVDSGMGAGCEFIKV--ARRGTADF--ANEARRLRWAAPY-LAVPRVLGVGVDGDWAWL 90
FRVD+ G ++K AR F EA RL W + L VP V+ GV AWL
Sbjct 35 FRVDTER-RGAYYVKTTPAREADTRFHPVTEAERLEWLRGHGLPVPEVVDAGVRDGLAWL 93
Query 91 HTDALPGLSAVHPRWRASPQVAVPALGAGLRTLHDSLPVHSCPFDWSTASRLAKLAPARR 150
T ALPG A P W A+ + V + A +LPV CPFD + LA A R
Sbjct 94 VTSALPGRPASGP-WPAAERPRVVRVLAEAAAALHALPVGGCPFDGRLRTSLAWAETAAR 152
Query 151 ----------------------AELGDSP-PVDRLVVCHGDACSPNTILD-DTGRCCGHV 186
AEL +P P + +VVCHGD C N ++D DT G +
Sbjct 153 TGRVDLDDLDEHHRGWSAGRLLAELRGTPAPPEDVVVCHGDLCPDNVLVDPDTLILTGII 212
Query 187 DFGNLGVADRWADLAVATLSLQWNFPDYPGQVRDDE-FFAAYGVAPDPARIDYYRRL 242
D G LG ADRW DLAV L +P R E F YG+ D + ++YR L
Sbjct 213 DAGRLGAADRWRDLAVVLRELGDEGGAWPAAERHGETFLRGYGITYDARKAEFYRLL 269
>gi|85813916|emb|CAF31545.1| putative fortimicin 3'-phosphotransferase (biosynthetic) [Micromonospora
olivasterospora]
Length=274
Score = 88.6 bits (218), Expect = 9e-16, Method: Compositional matrix adjust.
Identities = 95/271 (36%), Positives = 118/271 (44%), Gaps = 36/271 (13%)
Query 4 PSSPPALPAIVARFAVGRPVRAVWVNELGGVTFRVDSGMGAGCEFIKVARRGTADF---- 59
PS PPA A+ R + V G V RVD A RR D
Sbjct 4 PSRPPA--ALAGRLRLDGAWTHVASRSYGTVIHRVDGRRHAFYVKTTPPRRDN-DLRFHP 60
Query 60 ANEARRLRW-AAPYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGA 118
+EA RL+W AA V V+ VG D + WL T A+ G SA P V + +
Sbjct 61 GSEAERLQWLAARGFPVAEVVDVGGDDELMWLVTTAVEGRSAAGPWATHERPVVLDVVAD 120
Query 119 GLRTLHDSLPVHSCPFDWSTA-----SRLA---------KLAPARRA--------ELGDS 156
+R LH+ LP+ CPFD + A +RLA L P R EL +
Sbjct 121 VVRALHE-LPLTDCPFDRTLAVTLPLARLAAEMGWIDLDDLDPQHRGWSARQLLDELTAT 179
Query 157 PP--VDRLVVCHGDACSPNTILD-DTGRCCGHVDFGNLGVADRWADLAVATLSLQWNFPD 213
PP V+ LVVCHGD C N ++ DT G +D G LG ADRW DLA+A + D
Sbjct 180 PPPAVEDLVVCHGDPCLDNFLVAPDTLALTGILDVGRLGAADRWKDLAIAVRDVTEQCAD 239
Query 214 Y--PGQVRDDEFFAAYGVAPDPARIDYYRRL 242
GQ F A YG PD + +YR L
Sbjct 240 EGDEGQAAAARFLARYGARPDAGKDRFYRLL 270
>gi|254237006|ref|ZP_04930329.1| aminoglycoside 3'-phosphotransferase type IIb [Pseudomonas aeruginosa
C3719]
gi|126168937|gb|EAZ54448.1| aminoglycoside 3'-phosphotransferase type IIb [Pseudomonas aeruginosa
C3719]
Length=268
Score = 87.8 bits (216), Expect = 1e-15, Method: Compositional matrix adjust.
Identities = 85/260 (33%), Positives = 108/260 (42%), Gaps = 51/260 (19%)
Query 25 AVWVNELGGVTFRVDSGMGAGC-----EFIKVARRGT-----------ADFANEARRLRW 68
A W + L G +R G G GC ++ ARR T A+ E RLRW
Sbjct 14 ATWADYLAGYRWR---GQGEGCSAATVHRLEAARRPTLFVKKEVLSAHAELPAEIARLRW 70
Query 69 A-APYLAVPRVLGVGVDGDWAWLHTDALPG--LSAVHPRWRASPQVAVPALGAGLRTLHD 125
+ P+VL WL A+PG LSA+ R P+ V + A LR LHD
Sbjct 71 LHGAGIDCPQVLNETQSDGRQWLLMSAVPGDTLSALAQRGELEPERLVRLVAAALRRLHD 130
Query 126 SLPVHSCPFDWSTASRLAKLAPARRAELGDS-----------------------PPVDRL 162
P +CPFD RL + A L D P V+ L
Sbjct 131 LDPA-ACPFDHRLERRLDTVRQRVEAGLVDEADFDDDHRGRSATELYRLLLDRRPAVEDL 189
Query 163 VVCHGDACSPNTILDDTGRCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVRDDE 222
VV HGDAC PN +L + R G +D G LGVADR DLA+A ++ G +
Sbjct 190 VVAHGDACLPN-LLAEGRRFSGFIDCGRLGVADRHQDLALAARDIEAEL----GAAWAEA 244
Query 223 FFAAYGVAPDPARIDYYRRL 242
F YG D R+ Y+R L
Sbjct 245 FLVEYGGDIDGERLAYFRLL 264
>gi|125466|sp|P00555.1|KKA5_STRFR RecName: Full=Aminoglycoside 3'-phosphotransferase; AltName:
Full=APH(3')V; AltName: Full=Kanamycin kinase, type V; AltName:
Full=Neomycin-kanamycin phosphotransferase type V
gi|153161|gb|AAA26699.1| aminoglycoside phosphotransferase [Streptomyces fradiae]
Length=268
Score = 87.8 bits (216), Expect = 1e-15, Method: Compositional matrix adjust.
Identities = 74/213 (35%), Positives = 101/213 (48%), Gaps = 31/213 (14%)
Query 58 DFANEARRLRWAAPY-LAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAV-PA 115
D + EA RL W + + VPRV+ G D AWL T+A+PG++A W + AV A
Sbjct 55 DLSGEADRLEWLHRHGIPVPRVVERGADDTAAWLVTEAVPGVAAAE-EWPEHQRFAVVEA 113
Query 116 LGAGLRTLHDSLPVHSCPFDWSTASRLAK--------------LAPARRAELGDS----- 156
+ R LH+ LPV CP D + +A+ L R GD
Sbjct 114 MAELARALHE-LPVEDCPSDRRLDAAVAEARRNVAEGLVDLDDLQEERAGWTGDQLLAEL 172
Query 157 ----PPVDRLVVCHGDACSPNTILDDTGRC--CGHVDFGNLGVADRWADLAVATLSLQWN 210
P + LVVCHGD C PN +L D G C G +D G LGVADR AD+A+A L+ +
Sbjct 173 DRTRPEKEDLVVCHGDLC-PNNVLLDPGTCRVTGVIDVGRLGVADRHADIALAARELEID 231
Query 211 FPDYPGQVRDDEFFAAYGVAP-DPARIDYYRRL 242
+ G + F YG D ++ +Y+ L
Sbjct 232 EDPWFGPAYAERFLERYGAHRVDKEKLAFYQLL 264
>gi|953191|emb|CAA62365.1| aminoglycoside phosphotransferase (3')IIps [Pseudomonas aeruginosa]
Length=268
Score = 87.0 bits (214), Expect = 2e-15, Method: Compositional matrix adjust.
Identities = 84/260 (33%), Positives = 108/260 (42%), Gaps = 51/260 (19%)
Query 25 AVWVNELGGVTFRVDSGMGAGC-----EFIKVARRGT-----------ADFANEARRLRW 68
+ W + L G +R G G GC ++ ARR T A+ E RLRW
Sbjct 14 STWADYLAGYRWR---GQGEGCSAATVHRLEAARRPTLFVKQEVLSAHAELPAEIARLRW 70
Query 69 A-APYLAVPRVLGVGVDGDWAWLHTDALPG--LSAVHPRWRASPQVAVPALGAGLRTLHD 125
+ P+VL WL A+PG LSA+ R P+ V + A LR LHD
Sbjct 71 LHGAGIDCPQVLNETQSDGRQWLLMSAMPGDTLSALAQRDELEPERLVRLVAAALRRLHD 130
Query 126 SLPVHSCPFDWSTASRLAKLAPARRAELGDS-----------------------PPVDRL 162
P +CPFD RL + A L D P V+ L
Sbjct 131 LDPA-ACPFDHRLERRLDTVRQRVEAGLVDEADFDDDHRGRSATELYRLLLDRRPAVEDL 189
Query 163 VVCHGDACSPNTILDDTGRCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVRDDE 222
VV HGDAC PN +L + R G +D G LGVADR DLA+A ++ G +
Sbjct 190 VVAHGDACLPN-LLAEGRRFSGFIDCGRLGVADRHQDLALAARDIEAEL----GAAWAEA 244
Query 223 FFAAYGVAPDPARIDYYRRL 242
F YG D R+ Y+R L
Sbjct 245 FLVEYGGDIDGERLAYFRLL 264
>gi|190574079|ref|YP_001971924.1| putative aminoglycoside 3'-phosphotransferase [Stenotrophomonas
maltophilia K279a]
gi|190012001|emb|CAQ45623.1| putative aminoglycoside 3'-phosphotransferase [Stenotrophomonas
maltophilia K279a]
Length=267
Score = 87.0 bits (214), Expect = 2e-15, Method: Compositional matrix adjust.
Identities = 87/265 (33%), Positives = 108/265 (41%), Gaps = 47/265 (17%)
Query 17 FAVGRPVRAVWVNELGGVTFRVDSGMGAGCEFIKVARRGTAD-------------FANEA 63
F G P+ W L S + + +V R G AD +E
Sbjct 7 FTDGMPLPWAWQEALADARVERQSIGVSRADVARVQRPGQADAFVKSEVIDAFSELGDEI 66
Query 64 RRLRWAAPY-LAVPRVLGVGVDGDWAWLHTDALPGLS-AVHPRWRASPQVAVPALGAGLR 121
RLRW P V+ + WL ALPG A P +PQ V L LR
Sbjct 67 ARLRWLQEQGQPAPTVIATAEEAGRRWLLMSALPGRDLASSPEL--APQQLVELLADALR 124
Query 122 TLHDSLPVHSCPFDWSTASRLAKLAPAR------------RAELGDSPPV---------- 159
LH +LP+ +CPFD RL + A AR LG SP
Sbjct 125 GLH-ALPLAACPFDQRLHLRL-QAAQARVEAGLVDADDFDDERLGQSPQQVFAELCSTRP 182
Query 160 --DRLVVCHGDACSPNTILDDTGRCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQ 217
+ LVV HGDAC PN ++ + GR G +D G LGVADR+ DLA+A SL NF D
Sbjct 183 DHEDLVVSHGDACLPNLMVVE-GRFSGFIDCGRLGVADRYQDLALAARSLVHNFGDTRWV 241
Query 218 VRDDEFFAAYGVAPDPARIDYYRRL 242
F YG D R+ +YR L
Sbjct 242 A---PLFQRYGAVADERRLAFYRLL 263
>gi|15599314|ref|NP_252808.1| aminoglycoside 3'-phosphotransferase type IIb [Pseudomonas aeruginosa
PAO1]
gi|9950322|gb|AAG07506.1|AE004828_7 aminoglycoside 3'-phosphotransferase type IIb [Pseudomonas aeruginosa
PAO1]
Length=268
Score = 87.0 bits (214), Expect = 2e-15, Method: Compositional matrix adjust.
Identities = 84/260 (33%), Positives = 108/260 (42%), Gaps = 51/260 (19%)
Query 25 AVWVNELGGVTFRVDSGMGAGC-----EFIKVARRGT-----------ADFANEARRLRW 68
+ W + L G +R G G GC ++ ARR T A+ E RLRW
Sbjct 14 STWADYLAGYRWR---GQGEGCSAATVHRLEAARRPTLFVKQEVLSAHAELPAEIARLRW 70
Query 69 A-APYLAVPRVLGVGVDGDWAWLHTDALPG--LSAVHPRWRASPQVAVPALGAGLRTLHD 125
+ P+VL WL A+PG LSA+ R P+ V + A LR LHD
Sbjct 71 LHGAGIDCPQVLNETQSDGRQWLLMSAVPGDTLSALAQRGELEPERLVRLVAAALRRLHD 130
Query 126 SLPVHSCPFDWSTASRLAKLAPARRAELGDS-----------------------PPVDRL 162
P +CPFD RL + A L D P V+ L
Sbjct 131 LDPA-ACPFDHRLERRLDTVRQRVEAGLVDEADFDDDHRGRSATELYRLLLDRRPAVEDL 189
Query 163 VVCHGDACSPNTILDDTGRCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVRDDE 222
VV HGDAC PN +L + R G +D G LGVADR DLA+A ++ G +
Sbjct 190 VVAHGDACLPN-LLAEGRRFSGFIDCGRLGVADRHQDLALAARDIEAEL----GAAWAEA 244
Query 223 FFAAYGVAPDPARIDYYRRL 242
F YG D R+ Y+R L
Sbjct 245 FLVEYGGDIDGERLAYFRLL 264
>gi|218662247|ref|ZP_03518177.1| putative aminoglycoside 3'-phosphotransferase protein [Rhizobium
etli IE4771]
Length=280
Score = 86.7 bits (213), Expect = 3e-15, Method: Compositional matrix adjust.
Identities = 88/256 (35%), Positives = 116/256 (46%), Gaps = 34/256 (13%)
Query 10 LPAIVARFAVGRPVRAVWVNELGGVTFRVDSGMGAGCEFIKVARRG-TADFANEARRLRW 68
LPA + G + FR++ G G ++KV G + A+EA RL W
Sbjct 6 LPAALGERLTGYRFERDALGRSAASVFRLEGG-GLPALYLKVEAAGPFGELADEAARLGW 64
Query 69 -AAPYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGLRTLHDSL 127
A L P V+ DG WL ALPG + + +P V L A L LHD L
Sbjct 65 LKAVGLPCPDVIARESDGKRNWLLIVALPG-TDLASASALTPLTRVELLAAALSALHD-L 122
Query 128 PVHSCPFDWST----------------------ASRLAKLAPARRAELGDSPPVDR-LVV 164
P+ SCPFD A+RL + A A AEL P + LVV
Sbjct 123 PIASCPFDHRLENRLSVAKARMQAGIVDEEDFDAARLGQTAEALFAELESRRPGNEDLVV 182
Query 165 CHGDACSPNTILDDTGRCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVRDDEFF 224
HGDAC PN + D G+ G++D LGVADR D+A+A S+ NF G+ F
Sbjct 183 THGDACLPNFVACD-GQFSGYIDCSRLGVADRHQDIALACRSIASNF----GEALIQPFL 237
Query 225 AAYGV-APDPARIDYY 239
YG+ APD ++DY+
Sbjct 238 DRYGLPAPDAEKLDYF 253
>gi|116253139|ref|YP_768977.1| aminoglycoside 3'-phosphotransferase [Rhizobium leguminosarum
bv. viciae 3841]
gi|115257787|emb|CAK08885.1| putative aminoglycoside 3'-phosphotransferase [Rhizobium leguminosarum
bv. viciae 3841]
Length=265
Score = 86.7 bits (213), Expect = 3e-15, Method: Compositional matrix adjust.
Identities = 92/270 (35%), Positives = 124/270 (46%), Gaps = 48/270 (17%)
Query 4 PSSPPALPAIVA-----RFAVGRPVRAVWVNELGGVTFRVDSGMGAGCEFIKVARRG-TA 57
P S AL A +A R A+GR +V FR+++ +G ++KV G
Sbjct 9 PGSASALDARLAGYRFERDALGRSAASV---------FRLEA-LGLPTLYLKVEEVGPFG 58
Query 58 DFANEARRLRWA-APYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPAL 116
+ A+EA RLRW A L P ++ DG L +AL G S + +P V L
Sbjct 59 ELADEALRLRWVKASGLPCPDIIAEDSDGVRNRLLINALSG-SDLASASVLTPLGRVELL 117
Query 117 GAGLRTLHDSLPVHSCPFDWSTASRLAKLAPARRAELGDSPPVDR--------------- 161
A L LH LP+ SCPFD +R+A +A + D D
Sbjct 118 AAALLDLH-RLPIASCPFDHRLEARIAAAKARMQAGIVDETDFDETRLGKSAEALFAELE 176
Query 162 --------LVVCHGDACSPNTILDDTGRCCGHVDFGNLGVADRWADLAVATLSLQWNFPD 213
LVV HGDAC PN I + G G++D LGVADR+ +A+A S+ NF
Sbjct 177 SKRPDGADLVVTHGDACLPNFIASEDG-FSGYIDCSRLGVADRYQVIALACRSIAHNF-- 233
Query 214 YPGQVRDDEFFAAYGV-APDPARIDYYRRL 242
G+ F YG+ APDPAR+DYY+ L
Sbjct 234 --GEALVQPFLDRYGLTAPDPARLDYYQLL 261
>gi|15982219|emb|CAC86252.1| kanomycin resistance protein [Cloning vector pHELLSGATE]
gi|33320494|gb|AAQ05967.1| kanamycin resistance protein [Cloning vector pHellsgate 8]
gi|55829044|gb|AAV66537.1| neomycin phosphotransferase II [Binary vector pGA643]
gi|218157748|gb|ACK58633.1| neophosphotransferase [Carica papaya]
gi|228007503|emb|CAY39269.1| nptII [Cloning vector pKIGW]
gi|295983593|gb|ADG63448.1| neomycin phosphotransferase II [Binary vector pKM24KH]
Length=273
Score = 86.3 bits (212), Expect = 4e-15, Method: Compositional matrix adjust.
Identities = 89/282 (32%), Positives = 117/282 (42%), Gaps = 53/282 (18%)
Query 1 VSFPSSPPALPAIVARFAVGRPVRAVWVNELGG-------------VTFRVDSGMGAGCE 47
++ S +LP I AR G P A WV L G FR+ S G
Sbjct 1 MAITLSATSLP-ISARIRAGSP--AAWVERLFGYDWAQQTIGCSDAAVFRL-SAQGRPVL 56
Query 48 FIKVARRGTA-DFANEARRLRWAAPYLAVP--RVLGVGVDGDWAWLHTDALPGLSAVHPR 104
F+K G + +EA RL W A VP VL V + WL +PG +
Sbjct 57 FVKTDLSGALNELQDEAARLSWLATT-GVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSH 115
Query 105 WRASPQVAVPALGAGLRTLHDSLPVHSCPFDWSTASRLAKLAPARRAELGDSPPVDR--- 161
+P V + +R LH +L +CPFD R+ + A L D +D
Sbjct 116 L--APAEKVSIMADAMRRLH-TLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ 172
Query 162 --------------------LVVCHGDACSPNTILDDTGRCCGHVDFGNLGVADRWADLA 201
LVV HGDAC PN I+ + GR G +D G LGVADR+ D+A
Sbjct 173 GLAPAELFARLKARMPDGEDLVVTHGDACLPN-IMVENGRFSGFIDCGRLGVADRYQDIA 231
Query 202 VATLSLQWNFPDYPGQVRDDEFFAAYGV-APDPARIDYYRRL 242
+AT + + G D F YG+ APD RI +YR L
Sbjct 232 LAT----RDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLL 269
Lambda K H
0.323 0.138 0.463
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 362093846100
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Sep 5, 2011 4:36 AM
Number of letters in database: 5,219,829,388
Number of sequences in database: 15,229,318
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40