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virulence, detoxification, adaptation
lipid metabolism
conserved hypotheticals
pseudogenes
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
General annotation
TypeCDS
FunctionInvolved in the conversion of L-aspartate to homoserine (third step). Homoserine participates in the biosynthesis of threonine and then isoleucine and in the biosynthesis of methionine [catalytic activity: L-homoserine + NAD(P)(+) = L-aspartate 4-semialdehyde + NAD(P)H.]
ProductProbable homoserine dehydrogenase ThrA
CommentsRv1294, (MTCY373.14), len: 441 aa. Probable thrA (hom), homoserine dehydrogenase, highly similar to DHOM_MYCLE|P46806 from Mycobacterium leprae (441 aa), FASTA scores: opt: 2437, E():0, (89.5% identity in 438 aa overlap). Contains PS00017 ATP/GTP-binding site motif A; PS01042 Homoserine dehydrogenase signature. Belongs to the homoserine dehydrogenase family. Rv1294, (MTCY373.14), len: 441 aa. Probable thrA (hom), homoserine dehydrogenase, highly similar to DHOM_MYCLE|P46806 from Mycobacterium leprae (441 aa), FASTA scores: opt: 2437, E():0, (89.5% identity in 438 aa overlap). Contains PS00017 ATP/GTP-binding site motif A; PS01042 Homoserine dehydrogenase signature. Belongs to the homoserine dehydrogenase family.
Functional categoryIntermediary metabolism and respiration
ProteomicsIdentified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). Translational start site supported by proteomics data (See de Souza et al., 2011) (See Kelkar et al., 2011).
Mutationessential gene by Himar1-based transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by sequencing of Himar1-based transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS14493751450700+
RBS14493651449371+
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
      
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1294|thrA
VPGDEKPVGVAVLGLGNVGSEVVRIIENSAEDLAARVGAPLVLRGIGVRRVTTDRGVPIELLTDDIEELVAREDVDIVVEVMGPVEPSRKAILGALERGKSVVTANKALLATSTGELAQAAESAHVDLYFEAAVAGAIPVIRPLTQSLAGDTVLRVAGIVNGTTNYILSAMDSTGADYASALADASALGYAEADPTADVEGYDAAAKAAILASIAFHTRVTADDVYREGITKVTPADFGSAHALGCTIKLLSICERITTDEGSQRVSARVYPALVPLSHPLAAVNGAFNAVVVEAEAAGRLMFYGQGAGGAPTASAVTGDLVMAARNRVLGSRGPRESKYAQLPVAPMGFIETRYYVSMNVADKPGVLSAVAAEFAKREVSIAEVRQEGVVDEGGRRVGARIVVVTHLATDAALSETVDALDDLDVVQGVSSVIRLEGTGL