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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionSensor part of a two component regulatory system.
ProductProbable two component sensor kinase
CommentsRv3220c, (MTCY07D11.06), len: 501 aa. Probable sensor (probably histidine kinase), equivalent to Q9CCH8|ML0803 putative two-component system sensor kinase from Mycobacterium leprae (500 aa). Similar to others e.g. Q9F3M1|2SC7G11.01 putative histidine kinase (fragment) from Streptomyces coelicolor (372 aa), FASTA scores: opt: 1038, E(): 7.4e-56, (48.95% identity in 380 aa overlap); Q9A3K5|CC3198 sensor histidine kinase from Caulobacter crescentus (327 aa), FASTA scores: opt: 311, E(): 1.2e-11, (33.35% identity in 201 aa overlap) (similarity only in C-terminal part for this one); Q9A2T2|CC3474 putative sensor histidine kinase from Caulobacter crescentus (547 aa); etc. C-terminal half shows similarity to many sensor proteins, that respond to various stimuli from Methanobacterium thermoautotrophicum e.g. O26568|MTH468 sensory transduction histidine kinase (554 aa), FASTA scores: opt: 425, E(): 2.1e-18, (34.0% identity in 244 aa overlap); O26546|MTH446 sensory transduction regulatory protein (583 aa), FASTA scores: opt: 380, E(): 1.2e-15, (37.15% identity in 202 aa overlap); O26913|MTH823 sensory transduction regulatory protein (677 aa), FASTA scores: opt: 375, E(): 2.7e-15, (35.4% identity in 195 aa overlap); etc. Seems similar to other prokaryotic sensory transduction histidine kinases. Rv3220c, (MTCY07D11.06), len: 501 aa. Probable sensor (probably histidine kinase), equivalent to Q9CCH8|ML0803 putative two-component system sensor kinase from Mycobacterium leprae (500 aa). Similar to others e.g. Q9F3M1|2SC7G11.01 putative histidine kinase (fragment) from Streptomyces coelicolor (372 aa), FASTA scores: opt: 1038, E(): 7.4e-56, (48.95% identity in 380 aa overlap); Q9A3K5|CC3198 sensor histidine kinase from Caulobacter crescentus (327 aa), FASTA scores: opt: 311, E(): 1.2e-11, (33.35% identity in 201 aa overlap) (similarity only in C-terminal part for this one); Q9A2T2|CC3474 putative sensor histidine kinase from Caulobacter crescentus (547 aa); etc. C-terminal half shows similarity to many sensor proteins, that respond to various stimuli from Methanobacterium thermoautotrophicum e.g. O26568|MTH468 sensory transduction histidine kinase (554 aa), FASTA scores: opt: 425, E(): 2.1e-18, (34.0% identity in 244 aa overlap); O26546|MTH446 sensory transduction regulatory protein (583 aa), FASTA scores: opt: 380, E(): 1.2e-15, (37.15% identity in 202 aa overlap); O26913|MTH823 sensory transduction regulatory protein (677 aa), FASTA scores: opt: 375, E(): 2.7e-15, (35.4% identity in 195 aa overlap); etc. Seems similar to other prokaryotic sensory transduction histidine kinases.
Functional categoryRegulatory proteins
ProteomicsIdentified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011).
MutationNon-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in CDC1551 strain (see Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Virulence of M. tuberculosis H37Rv mutant in SCID mice is unchanged (See Parish et al., 2004).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS35960293597534-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
      
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3220c|Rv3220c
MSTLGDLLAEHTVLPGSAVDHLHAVVGEWQLLADLSFADYLMWVRRDDGVLVCVAQCRPNTGPTVVHTDAVGTVVAANSMPLVAATFSGGVPGREGAVGQQNSCQHDGHSVEVSPVRFGDQVVAVLTRHQPELAARRRSGHLETAYRLCATDLLRMLAEGTFPDAGDVAMSRSSPRAGDGFIRLDVDGVVSYASPNALSAYHRMGLTTELEGVNLIDATRPLISDPFEAHEVDEHVQDLLAGDGKGMRMEVDAGGATVLLRTLPLVVAGRNVGAAILIRDVTEVKRRDRALISKDATIREIHHRVKNNLQTVAALLRLQARRTSNAEGREALIESVRRVSSIALVHDALSMSVDEQVNLDEVIDRILPIMNDVASVDRPIRINRVGDLGVLDSDRATALIMVITELVQNAIEHAFDPAAAEGSVTIRAERSARWLDVVVHDDGLGLPQGFSLEKSDSLGLQIVRTLVSAELDGSLGMRDARERGTDVVLRVPVGRRGRLML