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virulence, detoxification, adaptation
lipid metabolism
conserved hypotheticals
pseudogenes
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
General annotation
TypeCDS
FunctionInvolved in GDP-mannose biosynthesis and biosynthesis of nucleotide-activated glycero-manno-heptose (D-alpha-D pathway): generates GDP-mannose and phosphate from GTP and alpha-D-mannose 1-phosphate. MANB product is needed for all mannosyl glycolipids and polysaccharides which, like rhamnosyl residues, are an important part of the mycobacterium envelope [catalytic activity: alpha-D-mannose 1-phosphate + GTP = GDP-mannose + phosphate].
ProductD-alpha-D-mannose-1-phosphate guanylyltransferase ManB (D-alpha-D-heptose-1-phosphate guanylyltransferase)
CommentsRv3264c, (MTCY71.04c), len: 359 aa. ManB (alternate gene name: hddC), D-alpha-D-mannose-1-phosphate guanylyltransferase (see citations below), equivalent to Q9CCK6|RMLA2|ML0753 putative sugar-phosphate nucleotidyl transferase from Mycobacterium leprae (358 aa), FASTA scores: opt: 2075, E(): 2.7e-115, (86.9% identity in 359 aa overlap). Also similar to others e.g. Q9KZK6|SCE34.20c putative nucleotide phosphorylase from Streptomyces coelicolor (360 aa), FASTA scores: opt: 1314, E(): 2.2e-70, (57.0% identity in 358 aa overlap); Q9KZP4|SC1A8A.08 putative mannose-1-phosphate guanyltransferase from Streptomyces coelicolor (831 aa), FASTA scores: opt: 699, E(): 8.6e-34, (34.45% identity in 354 aa overlap) (only similarity in N-terminus for this one); P74589|SLL1496 mannose-1-phosphate guanyltransferase from Synechocystis sp. strain PCC 6803 (843 aa), FASTA scores: opt: 692, E(): 2.3e-33, (35.1% identity in 342 aa overlap) (only similarity in N-terminus for this one too); BAB59222|TVG0079558 mannose-1-phosphate guanyltransferase from Thermoplasma volcanium (359 aa), FASTA scores: opt: 664, E(): 5.2e-32, (34.6% identity in 338 aa overlap); Q9ZTW5|GMP GDP-mannose pyrophosphorylase from Solanum tuberosum (Potato) (361 aa), FASTA scores: opt: 636, E(): 2.3e-30, (34.65% identity in 361 aa overlap); etc. Belongs to family 2 of mannose-6-phosphate isomerases. Note that previously known as rmlA2. Rv3264c, (MTCY71.04c), len: 359 aa. ManB (alternate gene name: hddC), D-alpha-D-mannose-1-phosphate guanylyltransferase (see citations below), equivalent to Q9CCK6|RMLA2|ML0753 putative sugar-phosphate nucleotidyl transferase from Mycobacterium leprae (358 aa), FASTA scores: opt: 2075, E(): 2.7e-115, (86.9% identity in 359 aa overlap). Also similar to others e.g. Q9KZK6|SCE34.20c putative nucleotide phosphorylase from Streptomyces coelicolor (360 aa), FASTA scores: opt: 1314, E(): 2.2e-70, (57.0% identity in 358 aa overlap); Q9KZP4|SC1A8A.08 putative mannose-1-phosphate guanyltransferase from Streptomyces coelicolor (831 aa), FASTA scores: opt: 699, E(): 8.6e-34, (34.45% identity in 354 aa overlap) (only similarity in N-terminus for this one); P74589|SLL1496 mannose-1-phosphate guanyltransferase from Synechocystis sp. strain PCC 6803 (843 aa), FASTA scores: opt: 692, E(): 2.3e-33, (35.1% identity in 342 aa overlap) (only similarity in N-terminus for this one too); BAB59222|TVG0079558 mannose-1-phosphate guanyltransferase from Thermoplasma volcanium (359 aa), FASTA scores: opt: 664, E(): 5.2e-32, (34.6% identity in 338 aa overlap); Q9ZTW5|GMP GDP-mannose pyrophosphorylase from Solanum tuberosum (Potato) (361 aa), FASTA scores: opt: 636, E(): 2.3e-30, (34.65% identity in 361 aa overlap); etc. Belongs to family 2 of mannose-6-phosphate isomerases. Note that previously known as rmlA2.
Functional categoryCell wall and cell processes
ProteomicsIdentified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). Translational start site supported by proteomics data (See de Souza et al., 2011) (See Kelkar et al., 2011).
Mutationessential gene by Himar1-based transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by sequencing of Himar1-based transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS36448983645977-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
      
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3264c|manB
LATHQVDAVVLVGGKGTRLRPLTLSAPKPMLPTAGLPFLTHLLSRIAAAGIEHVILGTSYKPAVFEAEFGDGSALGLQIEYVTEEHPLGTGGGIANVAGKLRNDTAMVFNGDVLSGADLAQLLDFHRSNRADVTLQLVRVGDPRAFGCVPTDEEDRVVAFLEKTEDPPTDQINAGCYVFERNVIDRIPQGREVSVEREVFPALLADGDCKIYGYVDASYWRDMGTPEDFVRGSADLVRGIAPSPALRGHRGEQLVHDGAAVSPGALLIGGTVVGRGAEIGPGTRLDGAVIFDGVRVEAGCVIERSIIGFGARIGPRALIRDGVIGDGADIGARCELLSGARVWPGVFLPDGGIRYSSDV
      
Bibliography