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virulence, detoxification, adaptation
lipid metabolism
conserved hypotheticals
pseudogenes
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
General annotation
TypeCDS
FunctionCatalyzes the production of GABA [catalytic activity: L-glutamate = 4-aminobutanoate + CO(2)].
ProductProbable glutamate decarboxylase GadB
CommentsRv3432c, (MTCY77.04c), len: 460 aa. Probable gadB, glutamate decarboxylase, similar to many e.g. P73043|gad|SLL1641 from Synechocystis sp. strain PCC 6803 (467 aa), FASTA scores: opt: 1684, E(): 6.2e-99, (55.35% identity in 457 aa overlap); Q9X8J5|SCE9.23 from Streptomyces coelicolor (475 aa), FASTA scores: opt: 1650, E(): 8.9e-97, (57.4% identity in 446 aa overlap); Q9AQU4|gad from Oryza sativa (Rice) (501 aa), FASTA scores: opt: 1498, E(): 3.7e-87, (51.6% identity in 432 aa overlap); Q07346|DCE_PETHY from Petunia hybrida (Petunia) (500 aa), FASTA scores: opt: 1485, E(): 2.5e-86, (51.15% identity in 437 aa overlap); etc. Belongs to group II decarboxylases (DDC, gad, HDC and TYRDC). Rv3432c, (MTCY77.04c), len: 460 aa. Probable gadB, glutamate decarboxylase, similar to many e.g. P73043|gad|SLL1641 from Synechocystis sp. strain PCC 6803 (467 aa), FASTA scores: opt: 1684, E(): 6.2e-99, (55.35% identity in 457 aa overlap); Q9X8J5|SCE9.23 from Streptomyces coelicolor (475 aa), FASTA scores: opt: 1650, E(): 8.9e-97, (57.4% identity in 446 aa overlap); Q9AQU4|gad from Oryza sativa (Rice) (501 aa), FASTA scores: opt: 1498, E(): 3.7e-87, (51.6% identity in 432 aa overlap); Q07346|DCE_PETHY from Petunia hybrida (Petunia) (500 aa), FASTA scores: opt: 1485, E(): 2.5e-86, (51.15% identity in 437 aa overlap); etc. Belongs to group II decarboxylases (DDC, gad, HDC and TYRDC).
Functional categoryIntermediary metabolism and respiration
ProteomicsIdentified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011).
Mutationnon essential gene by Himar1-based transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by sequencing of Himar1-based transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS38503723851754-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
      
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3432c|gadB
VSRSHPSVPAHSIAPAYTGRMFTAPVPALRMPDESMDPEAAYRFIHDELMLDGSSRLNLATFVTTWMDPEAEKLMAETFDKNMIDKDEYPATAAIEARCVSMVADLFHAEGLRDHDPTSATGVSTIGSSEAVMLGGLALKWRWRQRVGSWKGRMPNLVMGSNVQVVWEKFCRYFDVEPRYLPMERGRYVITPEQVLAAVDENTIGVVAILGTTYTGELEPIAEICAALDKLAAGGGVDVPVHVDAASGGFVVPFLHPDLVWDFRLPRVVSINVSGHKYGLTYPGVGFVVWRGPEHLPEDLVFRVNYLGGDMPTFTLNFSRPGNQVVGQYYNFLRLGRDGYTKVMQALSHTARWLGDQLREVDHCEVISDGSAIPVVSFRLAGDRGYTEFDVSHELRTFGWQVPAYTMPDNATDVAVLRIVVREGLSADLARALHDDAVTALAALDKVKPGGHFDAQHFAH