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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionInvolved in translation mechanism. This protein is one of the assembly proteins of the 50S ribosomal subunit.
Product30S ribosomal protein S9 RpsI
CommentsRv3442c, (MTCY77.14c), len: 151 aa. rpsI, 30S ribosomal protein S9, equivalent to P40828|RS9_MYCLE|ML0365|B229_C2_191 30S ribosomal protein S9 (153 aa), FASTA scores: opt: 800, E(): 2.1e-42, (83.85% identity in 155 aa overlap). Also highly similar to others e.g. Q53875|RS9_STRCO|SC6G4.13 from Streptomyces coelicolor (170 aa), FASTA scores: opt: 533, E(): 5.7e-26, (60.75% identity in 135 aa overlap); Q9KGD4|RPSI|BH0169 (BS10) from Bacillus halodurans (130 aa), FASTA scores: opt: 469, E(): 3.8e-22, (58.65% identity in 121 aa overlap); Q9CDG7|RPSI from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (130 aa), FASTA scores: opt: 451, E(): 4.9e-21, (58.65% identity in 121 aa overlap); P07842|RS9_BACST|RPSI from Bacillus stearothermophilus (129 aa), FASTA scores: opt: 448, E(): 7.4e-21, (54.55% identity in 121 aa overlap); etc. Contains PS00360 Ribosomal protein S9 signature. Belongs to the S9P family of ribosomal proteins.
Functional categoryInformation pathways
ProteomicsIdentified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified in the membrane fraction of M. tuberculosis H37Rv using nanoLC-MS/MS (See Xiong et al., 2005). Identified in both the aqueous and detergent phases of Triton X-114 extracts of M. tuberculosis H37Rv membranes using 1-DGE, CEGE, and MALDI-TOF-MS (See Sinha et al., 2005). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011).
MutantEssential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS38614953861950-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3442c|rpsI
MTETTPAPQTPAAPAGPAQSFVLERPIQTVGRRKEAVVRVRLVPGTGKFDLNGRSLEDYFPNKVHQQLIKAPLVTVDRVESFDIFAHLGGGGPSGQAGALRLGIARALILVSPEDRPALKKAGFLTRDPRATERKKYGLKKARKAPQYSKR