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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
intermediary metabolism and respiration
regulatory proteins
conserved hypotheticals
lipid metabolism
General annotation
FunctionInvolved in dTDP-L-rhamnose biosynthesis [catalytic activity: dTDP-glucose = dTDP-4-dehydro-6-deoxy-D-glucose + H(2)O].
ProductdTDP-glucose 4,6-dehydratase RmlB
CommentsRv3464, (MTCY13E12.17), len: 331 aa. RmlB (alternate gene name: rfbB), dTDP-glucose-4,6-dehydratase (see citations below), nearly identical to Q50556|RMLB rhamnose biosynthesis protein from Mycobacterium tuberculosis (329 aa) (previously rfbB, now known as rmlB). Equivalent to Q9CBH7|RMLB|ML1964 dTDP-glucose 4,6-dehydratase (alias Q9X7A3|RMLB putative dTDP-(glucose or rhamnose)-4,6-dehydratase (331 aa)) from Mycobacterium leprae (333 aa), FASTA scores: opt: 1925, E(): 1.9e-112, (84.0% identity in 331 aa overlap). Also highly similar to others e.g. Q9UZH2|RFBB|PAB0785 from Pyrococcus abyssi (333 aa), FASTA scores: opt: 1115, E(): 4.2e-62, (51.55% identity in 322 aa overlap); O27817|MTH1789 from Methanobacterium thermoautotrophicum (336 aa), FASTA scores: opt: 1104, E(): 2.1e-61, (51.65% identity in 331 aa overlap); BAB60064|TVG0950610 from Thermoplasma volcanium (318 aa), FASTA scores: opt: 1102, E(): 2.6e-61, (49.65% identity in 310 aa overlap); etc. Also related to P72050|MTCY13D12.18|RV3784 hypothetical 36.3 KDA protein (similar to galactowaldenases from eukaryotic and prokaryotic origin) from Mycobacterium tuberculosis (326 aa), FASTA scores: E(): 1.4e-26, (33.8% identity in 320 aa overlap).
Functional categoryIntermediary metabolism and respiration
ProteomicsIdentified in the cell wall fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011).
TranscriptomicsmRNA identified by microarray analysis and down-regulated after 24h and 96h of starvation (see Betts et al., 2002).
MutantEssential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). M. smegmatis knockout shows rmlB is essential for growth (See Li et al., 2006).
Check for mutants available at TARGET website
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3464|rmlB