Refseq_ID Mycobrowser_Version Feature Start Stop Score Strand Frame Locus Name Function Product Comments UniProt_AC Is_Pseudogene Functional_Category Orthologues M. leprae Orthologues M. marinum Orthologues M. smegmatis Orthologues M. bovis NC_000962.3 Mycobrowser_v2 ncRNA 4099386 4099478 . - 0 MTB000058 B11 Unknown Putative small regulatory RNA B11, putative small regulatory RNA (See Arnvig and Young, 2009; DiChiara et al., 2010). No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 547344 547517 . - 0 Rv0456B mazE1 Unknown Possible antitoxin MazE1 Rv0456B, len: 57 aa. Possible mazE1, antitoxin, part of toxin-antitoxin (TA) operon with Rv0456A (See Pandey and Gerdes, 2005; Zhu et al., 2006). P0CL57 No virulence, detoxification, adaptation NC_000962.3 Mycobrowser_v2 CDS 883451 884050 . - 0 Rv0789c Rv0789c Unknown Hypothetical protein Rv0789c, (MTCY369.33c), len: 199 aa. Hypothetical unknown protein. P71842 No conserved hypotheticals ML2218 MMAR_4896 Mb0813c NC_000962.3 Mycobrowser_v2 CDS 3533913 3534395 . - 0 Rv3165c Rv3165c Unknown Unknown protein Rv3165c, (MTV014.09)c, len: 160 aa. Unknown protein. O53315 No conserved hypotheticals ML0653 MMAR_1398 MSMEG_3868 Mb3190c NC_000962.3 Mycobrowser_v2 CDS 4013511 4014041 . + 0 Rv3572 Rv3572 Unknown Unknown protein Rv3572, (MTCY06G11.19), len: 176 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). P96854 No conserved hypotheticals ML0341 MMAR_5067 MSMEG_6040 Mb3603 NC_000962.3 Mycobrowser_v2 CDS 376573 377055 . - 0 Rv0307c Rv0307c Unknown Unknown protein Rv0307c, (MTCY63.12c), len: 160 aa. Unknown protein. O07234 No conserved hypotheticals ML2524 MMAR_0557 MSMEG_0632 Mb0315c NC_000962.3 Mycobrowser_v2 CDS 474940 475344 . + 0 Rv0395 Rv0395 Unknown Hypothetical protein Rv0395, (MTCY04D9.07), len: 134 aa. Hypothetical unknown protein. P95203 No conserved hypotheticals Mb0401 NC_000962.3 Mycobrowser_v2 tRNA 658109 658189 . + 0 MTB000005 tyrT - tRNA-Tyr (GTA) tyrT, tRNA-Tyr, anticodon gta, length = 81 No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 665042 665383 . - 0 Rv0572c Rv0572c Unknown Hypothetical protein Rv0572c, (MTV039.10c), len: 113 aa. Hypothetical unknown protein. O53769 No conserved hypotheticals Mb0587c NC_000962.3 Mycobrowser_v2 tRNA 733524 733596 . + 0 MTB000008 trpT - tRNA-Trp (CCA) trpT, tRNA-Trp, anticodon cca, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v2 tRNA 1113511 1113583 . + 0 MTB000015 alaV - tRNA-Ala (CGC) alaV, tRNA-Ala, anticodon cgc, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v2 tRNA 1446193 1446265 . - 0 MTB000018 argV - tRNA-Arg (CCG) argV, tRNA-Arg, anticodon ccg, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v2 tRNA 1512728 1512811 . + 0 MTB000022 leuW - tRNA-Leu (TAG) leuW, tRNA-Leu, anticodon tag, length = 84 No stable RNAs NC_000962.3 Mycobrowser_v2 RBS 1612242 1612245 . + 0 Rv1434 Rv1434 Unknown Hypothetical protein Rv1434, (MTCY493.20c), len: 45 aa. Hypothetical unknown protein. O06824 No conserved hypotheticals Mb1469 NC_000962.3 Mycobrowser_v2 CDS 1612256 1612393 . + 0 Rv1434 Rv1434 Unknown Hypothetical protein Rv1434, (MTCY493.20c), len: 45 aa. Hypothetical unknown protein. O06824 No conserved hypotheticals Mb1469 NC_000962.3 Mycobrowser_v2 CDS 1735976 1736212 . + 0 Rv1535 Rv1535 Unknown Unknown protein Rv1535, (MTCY07A7A.04), len: 78 aa. Unknown protein. O06180 No conserved hypotheticals MMAR_2348 Mb1562 NC_000962.3 Mycobrowser_v2 tRNA 1946613 1946686 . + 0 MTB000024 proT - tRNA-Pro (GGG) proT, tRNA-Pro, anticodon ggg, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 2075571 2075828 . + 0 Rv1831 Rv1831 Unknown Hypothetical protein Rv1831, (MTCY1A11.12c), len: 85 aa. Hypothetical unknown protein. P64899 No conserved hypotheticals Mb1862 NC_000962.3 Mycobrowser_v2 ncRNA 2139419 2139656 . + 0 MTB000056 AS1890 Unknown Putative small regulatory RNA AS1890, putative small regulatory RNA (See Arnvig and Young, 2009). Alternate 5'-ends at positions 2139466, 2139548, 2139594. No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 1280843 1281484 . - 0 Rv1154c Rv1154c Unknown Hypothetical protein Rv1154c, (MTCI65.21c), len: 213 aa. Hypothetical unknown protein, start uncertain. O06552 No conserved hypotheticals ML1509 MMAR_4299 MSMEG_5172 Mb1185c NC_000962.3 Mycobrowser_v2 CDS 2201277 2201579 . + 0 Rv1954A Rv1954A Unknown Hypothetical protein Rv1954A, len: 100 aa. Hypothetical unknown protein. P0CV86 No unknown NC_000962.3 Mycobrowser_v2 CDS 2314661 2314825 . - 0 Rv2057c rpmG1 Involved in translation 50S ribosomal protein L33 RpmG1 Rv2057c, (MTCY63A.03), len: 54 aa. rpmG1, 50S ribosomal protein L33, similar to many. Note that previously known as rpmG. P0A5W0 No information pathways MMAR_0291 MSMEG_6067 Mb2083c NC_000962.3 Mycobrowser_v2 RBS 2314837 2314841 . - 0 Rv2057c rpmG1 Involved in translation 50S ribosomal protein L33 RpmG1 Rv2057c, (MTCY63A.03), len: 54 aa. rpmG1, 50S ribosomal protein L33, similar to many. Note that previously known as rpmG. P0A5W0 No information pathways MMAR_0291 MSMEG_6067 Mb2083c NC_000962.3 Mycobrowser_v2 CDS 2333037 2333288 . - 0 Rv2076c Rv2076c Unknown Conserved hypothetical protein Rv2076c, (MTCY49.15c), len: 83 aa. Conserved hypothetical protein, similar to many. P64931 No conserved hypotheticals MMAR_5576 Mb2101c NC_000962.3 Mycobrowser_v2 tRNA 2510598 2510669 . - 0 MTB000027 valV - tRNA-Val (TAC) valV, tRNA-Val, anticodon tac, length = 72 No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 2528980 2529174 . - 0 Rv2255c Rv2255c Unknown Hypothetical protein Rv2255c, (MTV022.05c), len: 64 aa. Hypothetical unknown protein. O53529 No conserved hypotheticals Mb2279c NC_000962.3 Mycobrowser_v2 CDS 2719083 2719355 . + 0 Rv2422 Rv2422 Unknown Hypothetical protein Rv2422, (MTCY428.25c), len: 90 aa. Hypothetical unknown protein. P71926 No conserved hypotheticals ML1455 Mb2445 NC_000962.3 Mycobrowser_v2 tRNA 2794176 2794249 . + 0 MTB000032 argW - tRNA-Arg (TCT) argW, tRNA-Arg; anticodon tct, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v2 tRNA 2827854 2827926 . + 0 MTB000033 hisT - tRNA-His (GTG) hisT, tRNA-His, anticodon gtg, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v2 tRNA 2835494 2835566 . + 0 MTB000034 lysU - tRNA-Lys (CTT) lysU, tRNA-Lys, anticodon ctt, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v2 ncRNA 2881252 2881320 . + 0 MTB000068 mpr11 Unknown Fragment of putative small regulatory RNA mpr11, fragment of putative small regulatory RNA (See DiChiara et al., 2010), ends not mapped, 82-100 nt band detected by Northern blot in M. bovis BCG Pasteur. No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 3082352 3082756 . - 0 Rv2774c Rv2774c Unknown Hypothetical protein Rv2774c, (MTV002.39c), len: 134 aa. Hypothetical unknown protein. O33316 No conserved hypotheticals Mb2796c NC_000962.3 Mycobrowser_v2 CDS 3502945 3503277 . - 0 Rv3136A Rv3136A Unknown Conserved protein Rv3136A, len: 110 aa. Conserved protein. No conserved hypotheticals NC_000962.3 Mycobrowser_v2 CDS 3566696 3566896 . - 0 Rv3196A Rv3196A Unknown Unknown protein Rv3196A, len: 66 aa. Unknown protein. Q8VJ55 No conserved hypotheticals MMAR_1367 Mb3219c NC_000962.3 Mycobrowser_v2 CDS 3556855 3557064 . + 0 Rv3190A Rv3190A Unknown Conserved protein Rv3190A, len: 69 aa. Conserved protein. No conserved hypotheticals NC_000962.3 Mycobrowser_v2 tRNA 4168345 4168430 . + 0 MTB000046 serV - tRNA-Ser (GGA) serV, tRNA-Ser, anticodon gga, length = 86 No stable RNAs NC_000962.3 Mycobrowser_v2 ncRNA 4317073 4317165 . + 0 MTB000080 MTS2975 Unknown Putative small regulatory RNA MTS2975, putative small regulatory RNA (See Arnvig et al., 2011), ends not mapped, ~100 bp band detected by Northern blot. No stable RNAs NC_000962.3 Mycobrowser_v2 rRNA 1471846 1473382 . + 0 MTB000019 rrs - Ribosomal RNA 16S rrs, 16s rRNA gene (alternate gene name: rrnS). No stable RNAs NC_000962.3 Mycobrowser_v2 tRNA 386204 386274 . - 0 MTB000004 glyU - tRNA-Gly (CCC) glyU, tRNA-Gly, anticodon ccc, length = 71 No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 2307293 2307517 . - 0 Rv2049c Rv2049c Unknown Conserved hypothetical protein Rv2049c, (MTV018.36c), len: 74 aa. Conserved hypothetical protein. O53491 No conserved hypotheticals ML1438 MSMEG_3857 Mb2075c NC_000962.3 Mycobrowser_v2 CDS 444844 445233 . - 0 Rv0367c Rv0367c Unknown Hypothetical protein Rv0367c, (MTV036.02c), len: 129 aa. Hypothetical unknown protein. O53702 No conserved hypotheticals MSMEG_3435 Mb0374c NC_000962.3 Mycobrowser_v2 RBS 445241 445245 . - 0 Rv0367c Rv0367c Unknown Hypothetical protein Rv0367c, (MTV036.02c), len: 129 aa. Hypothetical unknown protein. O53702 No conserved hypotheticals MSMEG_3435 Mb0374c NC_000962.3 Mycobrowser_v2 CDS 606149 606448 . + 0 Rv0514 Rv0514 Unknown Possible transmembrane protein Rv0514, (MTCY20G10.04), len: 99 aa. Possible transmembrane protein. O33359 No cell wall and cell processes MMAR_0847 MSMEG_0960 Mb0527 NC_000962.3 Mycobrowser_v2 CDS 710345 710587 . + 0 Rv0615 Rv0615 Unknown Probable integral membrane protein Rv0615, (MTCY19H5.06c), len: 80 aa. Probable integral membrane protein. O07762 No cell wall and cell processes MMAR_0115 Mb0632 NC_000962.3 Mycobrowser_v2 CDS 789157 789411 . - 0 Rv0689c Rv0689c Unknown Hypothetical protein Rv0689c, (MTCY210.06c), len: 84 aa. Hypothetical unknown protein. P95035 No conserved hypotheticals Mb0708c NC_000962.3 Mycobrowser_v2 tRNA 850642 850713 . - 0 MTB000009 thrV - tRNA-Thr (TGT) thrV, tRNA-Thr, anticodon tgt, length = 72 No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 2546839 2547087 . - 0 Rv2274A mazE8 Unknown Possible antitoxin MazE8 Rv2274A, len: 82 aa. Possible mazE8, antitoxin, part of toxin-antitoxin (TA) operon with Rv2274c (See Pandey and Gerdes, 2005). P0CL60 No virulence, detoxification, adaptation NC_000962.3 Mycobrowser_v2 RBS 1003791 1003796 . + 0 Rv0900 Rv0900 Unknown Possible membrane protein Rv0900, (MTCY31.28), len: 50 aa. Possible membrane protein, with hydrophobic domain from aa 4-26. P64755 No cell wall and cell processes Mb0924 NC_000962.3 Mycobrowser_v2 CDS 1003805 1003957 . + 0 Rv0900 Rv0900 Unknown Possible membrane protein Rv0900, (MTCY31.28), len: 50 aa. Possible membrane protein, with hydrophobic domain from aa 4-26. P64755 No cell wall and cell processes Mb0924 NC_000962.3 Mycobrowser_v2 tRNA 1138076 1138147 . - 0 MTB000016 glnT - tRNA-Gln (TTG) glnT, tRNA-Gln, anticodon ttg, length = 72 No stable RNAs NC_000962.3 Mycobrowser_v2 tRNA 1177396 1177469 . + 0 MTB000017 leuX - tRNA-Leu (TAA) leuX, tRNA-Leu, anticodon taa, length = 74. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). No stable RNAs NC_000962.3 Mycobrowser_v2 ncRNA 1220388 1220487 . - 0 MTB000074 MTS0858 Unknown Putative small regulatory RNA MTS0858, putative small regulatory RNA (See Arnvig et al., 2011), ends not mapped, ~100 bp band detected by Northern blot. No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 1484982 1485278 . + 0 Rv1322 Rv1322 Function unknown Conserved hypothetical protein Rv1322, (MTCY130.07), len: 98 aa. Conserved hypothetical protein. P64805 No conserved hypotheticals ML1156 MSMEG_4922 Mb1356 NC_000962.3 Mycobrowser_v2 ncRNA 1535417 1535716 . - 0 MTB000065 mcr15 Unknown Putative small regulatory RNA mcr15, putative small regulatory RNA (See DiChiara et al., 2010). 5'-end mapped by 5'RLM-RACE in M. bovis BGC Pasteur, 3'-end not mapped. No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 1774860 1775144 . - 0 Rv1567c Rv1567c Unknown Probable hypothetical membrane protein Rv1567c, (MTCY336.36), len: 94 aa. Probable membrane protein. O06623 No cell wall and cell processes ML1215 MMAR_2382 Mb1594c NC_000962.3 Mycobrowser_v2 CDS 1786307 1786528 . - 0 Rv1584c Rv1584c Unknown Possible PhiRv1 phage protein Rv1584c, (MTCY336.20), len: 73 aa. Possible phiRv1 phage protein (putative excisionase) (see citation below). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06606 No insertion seqs and phages Mb1610c NC_000962.3 Mycobrowser_v2 RBS 1786535 1786540 . - 0 Rv1584c Rv1584c Unknown Possible PhiRv1 phage protein Rv1584c, (MTCY336.20), len: 73 aa. Possible phiRv1 phage protein (putative excisionase) (see citation below). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06606 No insertion seqs and phages Mb1610c NC_000962.3 Mycobrowser_v2 CDS 1815253 1815693 . + 0 Rv1615 Rv1615 Unknown Probable membrane protein Rv1615, (MTCY01B2.07), len: 146 aa. Probable membrane protein O06132 No cell wall and cell processes MMAR_2417 MSMEG_3223 Mb1641 NC_000962.3 Mycobrowser_v2 tRNA 1828015 1828088 . - 0 MTB000023 leuV - tRNA-Leu (CAA) leuV, tRNA-Leu, anticodon caa, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 2402507 2402722 . - 0 Rv2142A parD2 Unknown Possible antitoxin ParD2 Rv2142A, len: 71 aa. Possible parD2, antitoxin, part of toxin-antitoxin (TA) operon with Rv2142c (See Pandey and Gerdes, 2005). P0CW73 No virulence, detoxification, adaptation NC_000962.3 Mycobrowser_v2 CDS 1950632 1951051 . - 0 Rv1724c Rv1724c Unknown Hypothetical protein Rv1724c, (MTCY04C12.09c), len: 139 aa. Hypothetical unknown protein. P71982 No conserved hypotheticals Mb1753c NC_000962.3 Mycobrowser_v2 RBS 2006627 2006629 . + 0 Rv1772 Rv1772 Unknown Hypothetical protein Rv1772, (MTCY28.38), len: 103 aa. Hypothetical unknown protein. O06805 No conserved hypotheticals Mb1801 NC_000962.3 Mycobrowser_v2 CDS 2006636 2006947 . + 0 Rv1772 Rv1772 Unknown Hypothetical protein Rv1772, (MTCY28.38), len: 103 aa. Hypothetical unknown protein. O06805 No conserved hypotheticals Mb1801 NC_000962.3 Mycobrowser_v2 CDS 2012081 2012530 . - 0 Rv1778c Rv1778c Unknown Unknown protein Rv1778c, (MTCY25C11.05c), len: 149 aa. Unknown protein. O33181 No conserved hypotheticals Mb1807c NC_000962.3 Mycobrowser_v2 RBS 2012535 2012539 . - 0 Rv1778c Rv1778c Unknown Unknown protein Rv1778c, (MTCY25C11.05c), len: 149 aa. Unknown protein. O33181 No conserved hypotheticals Mb1807c NC_000962.3 Mycobrowser_v2 ncRNA 2882185 2882276 . + 0 MTB000069 mpr12 Unknown Fragment of putative small regulatory RNA mpr12, fragment of putative small regulatory RNA (See DiChiara et al., 2010), ends not mapped, ~118 nt band detected by Northern blot in M. bovis BCG Pasteur. No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 2159921 2160328 . - 0 Rv1914c Rv1914c Unknown Unknown protein Rv1914c, (MTCY180.04), len: 135 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). O07719 No conserved hypotheticals MMAR_2820 Mb1949c NC_000962.3 Mycobrowser_v2 CDS 2202584 2203129 . + 0 Rv1957 Rv1957 Unknown Hypothetical protein Rv1957, (MTCY09F9.07c), len: 181 aa. Hypothetical unknown protein. P95257 No conserved hypotheticals Mb1992 NC_000962.3 Mycobrowser_v2 CDS 2256084 2256428 . - 0 Rv2007c fdxA Involved in electron transfer. Ferredoxin FdxA Rv2007c, (MTCY39.10), len: 114 aa. FdxA, ferredoxin, similar to many e.g. FER_MYCSM P00215 ferredoxin, Mycobacterium smegmatis (106 aa). P64122 No intermediary metabolism and respiration MMAR_2080,MMAR_2994 MSMEG_1124 Mb2030c NC_000962.3 Mycobrowser_v2 CDS 2280240 2281082 . - 0 Rv2033c Rv2033c Unknown Conserved hypothetical protein Rv2033c, (MTV018.20), len: 280 aa. Conserved hypothetical protein, similar to many. O53477 No conserved hypotheticals ML1422 MMAR_3007 MSMEG_3020 Mb2059c NC_000962.3 Mycobrowser_v2 CDS 2287884 2288681 . - 0 Rv2042c Rv2042c Unknown Conserved protein Rv2042c, (MTV018.29c), len: 265 aa. Conserved protein, similar to many. O53486 No conserved hypotheticals ML1431 MMAR_3015 MSMEG_3684 Mb2068c NC_000962.3 Mycobrowser_v2 CDS 2327491 2328225 . - 0 Rv2070c cobK Involved in cobalamin biosynthesis. Precorrin-6X reductase CobK Rv2070c, (MTCY49.09c), len: 244 aa. CobK, precorrin-6x reductase, similar to many. Q10680 No intermediary metabolism and respiration ML1449 MMAR_3052 MSMEG_3875 Mb2096c NC_000962.3 Mycobrowser_v2 CDS 2372630 2373823 . + 0 Rv2113 Rv2113 Unknown Probable integral membrane protein Rv2113, (MTCY261.09), len: 397 aa. Probable integral membrane protein. O33248 No cell wall and cell processes ML1318 MMAR_3089 Mb2137 NC_000962.3 Mycobrowser_v2 CDS 2390085 2390288 . + 0 Rv2128 Rv2128 Unknown Conserved transmembrane protein Rv2128, (MTCY26.27), len: 67 aa. Conserved transmembrane protein, similar to many. O33262 No cell wall and cell processes MMAR_4816 Mb2152 NC_000962.3 Mycobrowser_v2 CDS 2584486 2584755 . + 0 Rv2312 Rv2312 Unknown Hypothetical protein Rv2312, (MTCY3G12.22c), len: 89 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P64991 No conserved hypotheticals Mb2339 NC_000962.3 Mycobrowser_v2 CDS 2719597 2720643 . + 0 Rv2423 Rv2423 Unknown Hypothetical protein Rv2423, (MTCY428.24c), len: 348 aa. Hypothetical unknown protein. P71925 No conserved hypotheticals MMAR_3750 Mb2446 NC_000962.3 Mycobrowser_v2 CDS 2752848 2752994 . - 0 Rv2452c Rv2452c Unknown Hypothetical protein Rv2452c, (MTV008.08c), len: 48 aa. Hypothetical unknown protein (see citation below). O53179 No conserved hypotheticals Mb2479c NC_000962.3 Mycobrowser_v2 tRNA 2765331 2765404 . - 0 MTB000030 proU - tRNA-Pro (TGG) proU, tRNA-Pro; anticodon tgg, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v2 tRNA 2765541 2765611 . + 0 MTB000031 glyV - tRNA-Gly (TCC) glyV, tRNA-Gly; anticodon tcc, length = 71 No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 2926355 2926975 . + 0 Rv2597 Rv2597 Unknown Probable membrane protein Rv2597, (MTCY227.04c), len: 206 aa. Probable membrane protein. Predicted to be an outer membrane protein (See Song et al., 2008). P65029 No cell wall and cell processes ML0480 MMAR_2108 Mb2628 NC_000962.3 Mycobrowser_v2 CDS 2962470 2962712 . + 0 Rv2635 Rv2635 Unknown Hypothetical protein Rv2635, (MTCY441.05), len: 80 aa. Hypothetical unknown protein. P65037 No conserved hypotheticals Mb2668 NC_000962.3 Mycobrowser_v2 CDS 2976586 2976909 . - 0 Rv2653c Rv2653c Unknown Possible PhiRv2 prophage protein Rv2653c, (MTCY441.22c), len: 107 aa. Hypothetical unknown protein, possibly phiRv2 phage protein (see citation below). P71950 No insertion seqs and phages NC_000962.3 Mycobrowser_v2 CDS 2979326 2979688 . - 0 Rv2658c Rv2658c Unknown Possible prophage protein Rv2658c, (MTCY441.27c), len: 120 aa. Hypothetical unknown protein, probably phage protein. P71955 No insertion seqs and phages NC_000962.3 Mycobrowser_v2 CDS 3042542 3043018 . + 0 Rv2730 Rv2730 Unknown Hypothetical protein Rv2730, (MTCY174.10), len: 158 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O33235 No conserved hypotheticals Mb2749 NC_000962.3 Mycobrowser_v2 CDS 3020200 3020457 . - 0 Rv2706c Rv2706c Unknown Hypothetical protein Rv2706c, (MTCY05A6.27c), len: 85 aa (unlikely ORF). Hypothetical unknown protein. O07207 No conserved hypotheticals ML1019 Mb2725c NC_000962.3 Mycobrowser_v2 CDS 3115408 3115719 . + 0 Rv2809 Rv2809 Unknown Hypothetical protein Rv2809, (MTCY16B7.34c), len: 103 aa (questionable ORF). Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P71642 No conserved hypotheticals Mb2832 NC_000962.3 Mycobrowser_v2 CDS 3174747 3174995 . + 0 Rv2862A vapB23 Unknown Possible antitoxin VapB23 Rv2862A, len: 82 aa. Possible vapB23, antitoxin, part of toxin-antitoxin (TA) operon with Rv2863 (See Pandey and Gerdes, 2005). P0CW32 No virulence, detoxification, adaptation NC_000962.3 Mycobrowser_v2 CDS 3366644 3367267 . + 0 Rv3008 Rv3008 Unknown Hypothetical protein Rv3008, (MTV012.22), len: 207 aa (start uncertain). Hypothetical unknown protein. O53255 No conserved hypotheticals Mb3033 NC_000962.3 Mycobrowser_v2 tRNA 3348547 3348619 . - 0 MTB000039 gluU - tRNA-Glu (CTC) gluU, tRNA-Glu; anticodon ctc, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 3392812 3393201 . + 0 Rv3032A Rv3032A Unknown Conserved protein Rv3032A, len: 129 aa. Conserved protein. No conserved hypotheticals NC_000962.3 Mycobrowser_v2 CDS 3491808 3492122 . - 0 Rv3126c Rv3126c Unknown Hypothetical protein Rv3126c, (MTCY164.36c), unknown, len: 104 aa. Hypothetical unknown protein. Shortened version of MTCY164.36c, avoiding overlap. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O05799 No conserved hypotheticals Mb3149c NC_000962.3 Mycobrowser_v2 CDS 3540882 3541364 . - 0 Rv3172c Rv3172c Unknown Hypothetical protein Rv3172c, (MTV014.16c), len: 160 aa. Hypothetical unknown protein. O53322 No conserved hypotheticals ML0645 Mb3197c NC_000962.3 Mycobrowser_v2 RBS 3541372 3541375 . - 0 Rv3172c Rv3172c Unknown Hypothetical protein Rv3172c, (MTV014.16c), len: 160 aa. Hypothetical unknown protein. O53322 No conserved hypotheticals ML0645 Mb3197c NC_000962.3 Mycobrowser_v2 CDS 3611300 3611941 . + 0 Rv3235 Rv3235 Unknown Hypothetical alanine arginine proline rich protein Rv3235, (MTCY20B11.10), len: 213 aa. Hypothetical unknown ala-, arg-, pro-rich protein. O05880 No conserved hypotheticals ML0783 MMAR_1310 MSMEG_1880 Mb3263 NC_000962.3 Mycobrowser_v2 CDS 3908072 3908236 . + 0 Rv3489 Rv3489 Unknown Unknown protein Rv3489, (MTCY13E12.42), len: 54 aa. Unknown protein. No similarity with other proteins. O06352 No conserved hypotheticals MMAR_4977 MSMEG_5890 Mb3519 NC_000962.3 Mycobrowser_v2 RBS 3909879 3909885 . + 0 Rv3491 Rv3491 Unknown Unknown protein Rv3491, (MTCY13E12.44), len: 192 aa. Unknown protein. No significant homology with other proteins. O06354 No conserved hypotheticals ML2255 MMAR_4979 MSMEG_4353 Mb3521 NC_000962.3 Mycobrowser_v2 CDS 3909890 3910468 . + 0 Rv3491 Rv3491 Unknown Unknown protein Rv3491, (MTCY13E12.44), len: 192 aa. Unknown protein. No significant homology with other proteins. O06354 No conserved hypotheticals ML2255 MMAR_4979 MSMEG_4353 Mb3521 NC_000962.3 Mycobrowser_v2 RBS 4196712 4196715 . + 0 Rv3747 Rv3747 Function unknown Conserved protein Rv3747, (MTV025.095), len: 127 aa. Conserved protein, highly similar to downstream ORF O69715|Rv3748|MTV025.096 conserved hypothetical protein (119 aa), FASTA scores: opt: 494, E(): 6e-27, (64.4% identity in 118 aa overlap). O69714 No conserved hypotheticals MMAR_5287 Mb3773 NC_000962.3 Mycobrowser_v2 CDS 4196724 4197107 . + 0 Rv3747 Rv3747 Function unknown Conserved protein Rv3747, (MTV025.095), len: 127 aa. Conserved protein, highly similar to downstream ORF O69715|Rv3748|MTV025.096 conserved hypothetical protein (119 aa), FASTA scores: opt: 494, E(): 6e-27, (64.4% identity in 118 aa overlap). O69714 No conserved hypotheticals MMAR_5287 Mb3773 NC_000962.3 Mycobrowser_v2 CDS 3963605 3964054 . + 0 Rv3527 Rv3527 Unknown Hypothetical protein Rv3527, (MTCY03C7.29c), len: 149 aa. Hypothetical unknown protein. P71874 No conserved hypotheticals MMAR_5016 MSMEG_5927 Mb3557 NC_000962.3 Mycobrowser_v2 CDS 4008167 4008433 . - 0 Rv3566A Rv3566A Unknown Hypothetical protein Rv3566A, len: 88 aa. Hypothetical unknown protein. Q8VIX9 No conserved hypotheticals Mb3597c NC_000962.3 Mycobrowser_v2 CDS 4042952 4043035 . - 0 Rv3599c Rv3599c Unknown Hypothetical short protein Rv3599c, (MTCY07H7B.23), len: 27 aa. Hypothetical unknown protein. O06283 No unknown NC_000962.3 Mycobrowser_v2 tRNA 4081365 4081437 . - 0 MTB000044 thrU - tRNA-Thr (CGT) thrU, tRNA-Thr, anticodon cgt, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 4140239 4140463 . - 0 Rv3697A vapB48 Unknown Possible antitoxin VapB48 Rv3697A, len: 74 aa. Possible vapB48, antitoxin, part of toxin-antitoxin (TA) operon with Rv3697c, see Arcus et al. 2005. Similar to others in M. tuberculosis e.g. Rv3321c, Rv0748 P0CW36 No virulence, detoxification, adaptation NC_000962.3 Mycobrowser_v2 tRNA 4216865 4216937 . - 0 MTB000048 argU - tRNA-Arg (ACG) argU, tRNA-Arg, anticodon acg, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v2 tRNA 4222581 4222667 . - 0 MTB000050 serU - tRNA-Ser (TGA) serU, tRNA-Ser, anticodon tga, length = 87 No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 4305757 4306239 . + 0 Rv3831 Rv3831 Unknown Hypothetical protein Rv3831, (MTCY01A6.38c), len: 160 aa. Hypothetical unknown protein. P96247 No conserved hypotheticals ML0083 MMAR_5385 Mb3861 NC_000962.3 Mycobrowser_v2 ncRNA 4314798 4314891 . + 0 MTB000079 ncrMT3949 Unknown Fragment of putative small regulatory RNA ncrMT3949, fragment of putative small regulatory RNA (See Pelly et al., 2012), cloned from M. tuberculosis CDC1551; supported by RNA-seq in H37Rv (unpublished data). No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 4324683 4324967 . + 0 Rv3851 Rv3851 Unknown Possible membrane protein Rv3851, (MTCY01A6.17c), len: 94 aa. Possible membrane protein. P96226 No cell wall and cell processes Mb3881 NC_000962.3 Mycobrowser_v2 CDS 28362 29207 . + 0 Rv0024 Rv0024 Unknown. The P60 protein is a major extracellular protein may be involved in the invasion of host cells. Putative secreted protein P60-related protein Rv0024, (MTCY10H4.24), len: 281 aa. Putative secreted protein, p60 homologue, similar to many. Similar to Mycobacterium tuberculosis proteins Rv1477, Rv1478, Rv1566c, Rv2190c. Could belong to the E. coli NLPC / listeria P60 family. P71594 No virulence, detoxification, adaptation MMAR_0043 Mb0024,Mb0025 NC_000962.3 Mycobrowser_v2 RBS 63899 63904 . + 0 Rv0060 Rv0060 Function unknown Conserved hypothetical protein Rv0060, (MTV030.03), len: 352 aa. Conserved hypothetical protein. O53605 No conserved hypotheticals Mb0061 NC_000962.3 Mycobrowser_v2 CDS 63909 64967 . + 0 Rv0060 Rv0060 Function unknown Conserved hypothetical protein Rv0060, (MTV030.03), len: 352 aa. Conserved hypothetical protein. O53605 No conserved hypotheticals Mb0061 NC_000962.3 Mycobrowser_v2 CDS 165323 165826 . + 0 Rv0138 Rv0138 Function unknown Conserved hypothetical protein Rv0138, (MTCI5.12), len: 167 aa. Conserved hypothetical protein, showing weak similarity to Q10827|YT10_MYCTU hypothetical 17.0 KDA protein from Mycobacterium tuberculosis (147 aa), FASTA scores: opt: 131, E(): 0.047, (31.15% identity in 106 aa overlap). P96815 No conserved hypotheticals ML2646 MMAR_0348 MSMEG_6475 Mb0143 NC_000962.3 Mycobrowser_v2 CDS 186495 186623 . - 0 Rv0157A Rv0157A Unknown Conserved protein Rv0157A, len: 42 aa. Conserved protein, showing similarity to C-terminal part (aa 186-220) of O53976|Rv1975|MTV051.13 conserved hypothetical protein from Mycobacterium tuberculosis (221 aa), FASTA scores: opt: 173, E(): 3e-06, (62.5% identity in 40 aa overlap). No conserved hypotheticals NC_000962.3 Mycobrowser_v2 RBS 186629 186632 . - 0 Rv0157A Rv0157A Unknown Conserved protein Rv0157A, len: 42 aa. Conserved protein, showing similarity to C-terminal part (aa 186-220) of O53976|Rv1975|MTV051.13 conserved hypothetical protein from Mycobacterium tuberculosis (221 aa), FASTA scores: opt: 173, E(): 3e-06, (62.5% identity in 40 aa overlap). No conserved hypotheticals NC_000962.3 Mycobrowser_v2 CDS 1947416 1947643 . - 0 Rv1721c vapB12 Unknown Possible antitoxin VapB12 Rv1721c, (MTCY04C12.06c), len: 75 aa. Possible vapB12, antitoxin, part of toxin-antitoxin (TA) operon with Rv1720c (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others from Mycobacterium tuberculosis e.g. Rv0300|MTCY63.05|O07227 conserved hypothetical protein (73 aa). Start changed since original submission. P71979 No virulence, detoxification, adaptation Mb1750c NC_000962.3 Mycobrowser_v2 CDS 1690407 1690805 . + 0 Rv1499 Rv1499 Unknown Hypothetical protein Rv1499, (MTCY277.21), len: 132 aa. Hypothetical unknown protein; was initially longer but has been shortened (-24 aa) owing to overlap with Rv1498A. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P71780 No conserved hypotheticals Mb1537 NC_000962.3 Mycobrowser_v2 CDS 2337306 2337869 . + 0 Rv2080 lppJ Unknown Lipoprotein LppJ Rv2080, (MTCY49.19), len: 187 aa. LppJ, lipoprotein; contains prokayotic lipoprotein modification site (PS00013) and signal sequence at N-terminus. Q10688 No cell wall and cell processes MMAR_5467 NC_000962.3 Mycobrowser_v2 CDS 42433 43365 . - 0 Rv0040c mtc28 Unknown Secreted proline rich protein Mtc28 (proline rich 28 kDa antigen) Rv0040c, (MTCY21D4.03c), len: 310 aa. Mtc28, secreted proline rich 28 kDa antigen protein (has hydrophobic stretch at N-terminus) (see citation below). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P0A5Q6 No cell wall and cell processes ML0031 MMAR_0056 MSMEG_6919 Mb0041c NC_000962.3 Mycobrowser_v2 RBS 43375 43380 . - 0 Rv0040c mtc28 Unknown Secreted proline rich protein Mtc28 (proline rich 28 kDa antigen) Rv0040c, (MTCY21D4.03c), len: 310 aa. Mtc28, secreted proline rich 28 kDa antigen protein (has hydrophobic stretch at N-terminus) (see citation below). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P0A5Q6 No cell wall and cell processes ML0031 MMAR_0056 MSMEG_6919 Mb0041c NC_000962.3 Mycobrowser_v2 CDS 29722 31068 . + 0 Rv0026 Rv0026 Function unknown Conserved hypothetical protein Rv0026, (MTCY10H4.26), len: 448 aa. Conserved hypothetical protein, showing some similarity to other proteins from Mycobacterium tuberculosis: Rv0025 FASTA score: (35.4% identity in 113 aa overlap) and Rv0739 (268 aa), FASTA score: (32.4% identity in 142 aa overlap). P71596 No conserved hypotheticals MMAR_0045 Mb0027 NC_000962.3 Mycobrowser_v2 CDS 31189 31506 . + 0 Rv0027 Rv0027 Unknown Conserved hypothetical protein Rv0027, (MTCY10H4.27), len: 105 aa. Conserved hypothetical unknown protein. P64667 No conserved hypotheticals MMAR_0046 Mb0028 NC_000962.3 Mycobrowser_v2 CDS 31514 31819 . + 0 Rv0028 Rv0028 Unknown Conserved hypothetical protein Rv0028, (MTCY10H4.28), len: 101 aa. Conserved hypothetical unknown protein. P64669 No conserved hypotheticals MMAR_0047 Mb0029 NC_000962.3 Mycobrowser_v2 CDS 32057 33154 . + 0 Rv0029 Rv0029 Function unknown Conserved hypothetical protein Rv0029, (MTCY10H4.29), len: 365 aa. Conserved hypothetical protein, showing some similarity to other proteins from Mycobacterium tuberculosis e.g. C-terminal region of Rv2082; Rv3899c. P71599 No conserved hypotheticals MMAR_0048 Mb0030 NC_000962.3 Mycobrowser_v2 CDS 33224 33553 . + 0 Rv0030 Rv0030 Unknown Conserved hypothetical protein Rv0030, (MTCY10H4.30), len: 109 aa. Conserved hypothetical unknown protein. P64671 No conserved hypotheticals MMAR_0049 Mb0031 NC_000962.3 Mycobrowser_v2 CDS 52831 53244 . + 0 Rv0049 Rv0049 Function unknown Conserved hypothetical protein Rv0049, (MTCY21D4.12), len: 137 aa. Conserved hypothetical protein. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P64677 No conserved hypotheticals ML2689 MMAR_0068 MSMEG_6901 Mb0050 NC_000962.3 Mycobrowser_v2 CDS 33582 33794 . + 0 Rv0031 Rv0031 Normally, required for the transposition of an insertion element. Possible remnant of a transposase Rv0031, (MTCY10H4.31), len: 70 aa. Possible remnant of a transposase, showing partial similarity to mycobacterial transposases in a short overlap, e.g. Rv2791c|MTV002_57 (459 aa), FASTA score: (72.2% identity in 36 aa overlap); Rv2885c, Rv2978c, Rv3827c, etc. P71601 No insertion seqs and phages Mb0032 NC_000962.3 Mycobrowser_v2 CDS 41304 41912 . + 0 Rv0038 Rv0038 Function unknown Conserved protein Rv0038, (MTCY10H4.38), len: 202 aa. Conserved protein, similar to many. P67757 No conserved hypotheticals ML0028 MMAR_0053 MSMEG_6921 Mb0039 NC_000962.3 Mycobrowser_v2 CDS 42004 42351 . - 0 Rv0039c Rv0039c Unknown Possible conserved transmembrane protein Rv0039c, (MTCY21D4.02c, MTCY10H4.39c), len: 115 aa. Possible conserved transmembrane protein. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P71696 No cell wall and cell processes ML0030 MMAR_0055 MSMEG_6920 Mb0040c NC_000962.3 Mycobrowser_v2 RBS 42356 42360 . - 0 Rv0039c Rv0039c Unknown Possible conserved transmembrane protein Rv0039c, (MTCY21D4.02c, MTCY10H4.39c), len: 115 aa. Possible conserved transmembrane protein. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P71696 No cell wall and cell processes ML0030 MMAR_0055 MSMEG_6920 Mb0040c NC_000962.3 Mycobrowser_v2 CDS 65012 65350 . - 0 Rv0061c Rv0061c Unknown Hypothetical protein Rv0061c, len: 112 aa. Conserved hypothetical protein supported by RNA-seq data. Similar to MMAR_3839, 76% identity in 112 aa overlap. Replaces questionable ORF Rv0061 (MTV030.04). O53606 No conserved hypotheticals Mb0062 NC_000962.3 Mycobrowser_v2 rRNA 1476899 1477013 . + 0 MTB000021 rrf - Ribosomal RNA 5S rrf, 5S rRNA gene. Identical to Em_ba:MT5SRR, D10035 M.tuberculosis 5S rRNA, len: 116. No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 9914 10828 . + 0 Rv0007 Rv0007 Unknown Possible conserved membrane protein Rv0007, (MTCY10H4.05), len: 304 aa. Possible conserved membrane protein. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P71575 No cell wall and cell processes ML0007 MMAR_0007 MSMEG_0007 Mb0007 NC_000962.3 Mycobrowser_v2 tRNA 10887 10960 . + 0 MTB000001 ileT - tRNA-Ile (GAT) ileT, tRNA-Ile, anticodon gat, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v2 tRNA 11112 11184 . + 0 MTB000002 alaT - tRNA-Ala (TGC) alaT, tRNA-Ala, anticodon tgc, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 11874 12311 . - 0 Rv0008c Rv0008c Unknown Possible membrane protein Rv0008c, (MTCY10H4.07c), len: 145 aa. Possible membrane protein. P71577 No cell wall and cell processes ML0010 MMAR_0010 MSMEG_0023 Mb0008c NC_000962.3 Mycobrowser_v2 RBS 98469 98474 . + 0 Rv0090 Rv0090 Unknown Possible membrane protein Rv0090, (MTCY251.08), len: 256 aa. Possible membrane protein. Contains IPR014511 Protein of unknown function DUF2068, transmembrane, subgroup. P64683 No cell wall and cell processes ML2653 MMAR_0246 Mb0093 NC_000962.3 Mycobrowser_v2 CDS 98480 99250 . + 0 Rv0090 Rv0090 Unknown Possible membrane protein Rv0090, (MTCY251.08), len: 256 aa. Possible membrane protein. Contains IPR014511 Protein of unknown function DUF2068, transmembrane, subgroup. P64683 No cell wall and cell processes ML2653 MMAR_0246 Mb0093 NC_000962.3 Mycobrowser_v2 CDS 87798 88004 . - 0 Rv0078B Rv0078B Unknown Conserved protein Rv0078B, len: 68 aa. Conserved protein. No conserved hypotheticals NC_000962.3 Mycobrowser_v2 CDS 88204 89025 . + 0 Rv0079 Rv0079 Unknown Unknown protein Rv0079, (MTV030.23), len: 273 aa. Unknown protein. Predicted possible vaccine candidate (See Zvi et al., 2008). O53624 No conserved hypotheticals MMAR_3705 MSMEG_3935 Mb0082 NC_000962.3 Mycobrowser_v2 CDS 89022 89480 . + 0 Rv0080 Rv0080 Function unknown Conserved hypothetical protein Rv0080, (MTV030.24), len: 152 aa. Conserved hypothetical protein. Belongs to pyridoxine 5'-phosphate (PNP) oxidase-like (PNPOx-like) superfamily. O53625 No conserved hypotheticals MSMEG_5136 Mb0083 NC_000962.3 Mycobrowser_v2 CDS 2338065 2338505 . - 0 Rv2081c Rv2081c Unknown Conserved transmembrane protein Rv2081c, (MTCY49.20c), len: 146 aa. Conserved transmembrane protein, similar to many. Hydrophobic stretch from aa 32-54. Q10689 No cell wall and cell processes Mb2107c NC_000962.3 Mycobrowser_v2 CDS 162644 163249 . - 0 Rv0135c Rv0135c Could be involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0135c, (MTCI5.09c), len: 201 aa. Possible transcriptional regulator, weakly similar to others e.g. P32398|YHGD_BACSU hypothetical transcriptional regulator from Bacillus subtilis (191 aa), FASTA scores: opt: 145, E(): 0.0012, (21.0% identity in 162 aa overlap). P96812 No regulatory proteins MMAR_0345 MSMEG_6480 Mb0140c NC_000962.3 Mycobrowser_v2 CDS 12468 13016 . + 0 Rv0009 ppiA PPIases accelerate the folding of proteins [catalytic activity: cis-trans isomerization of proline imidic peptide bonds in oligopeptides]. Probable iron-regulated peptidyl-prolyl cis-trans isomerase A PpiA (PPIase A) (rotamase A) Rv0009, (MTCY10H4.08), len: 182 aa. Probable ppiA (alternate gene name: cfp22), iron-regulated peptidyl-prolyl cis-trans isomerase A. Belongs to the cyclophilin-type PPIase family. Alternative start codon has been suggested. P65762 No information pathways ML0011 MMAR_0011 MSMEG_0024 Mb0009 NC_000962.3 Mycobrowser_v2 CDS 13133 13558 . - 0 Rv0010c Rv0010c Unknown Probable conserved membrane protein Rv0010c, (MTCY10H4.10c), len: 141 aa. Probable conserved membrane protein. Belongs to superfamily DUF2581, conserved in the Actinomycetales. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P71580 No cell wall and cell processes ML0012 MMAR_0012 MSMEG_0025 Mb0010c NC_000962.3 Mycobrowser_v2 CDS 13714 13995 . - 0 Rv0011c Rv0011c Unknown Probable conserved transmembrane protein Rv0011c, (MTCY10H4.11c), len: 93 aa. Probable conserved transmembrane protein. Belongs to uncharacterized protein family UPF0233. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P67376 No cell wall and cell processes ML0013 MMAR_0013 MSMEG_0026 Mb0011c NC_000962.3 Mycobrowser_v2 CDS 14089 14877 . + 0 Rv0012 Rv0012 Unknown Probable conserved membrane protein Rv0012, (MTCY10H4.12), len: 262 aa. Probable conserved membrane protein. Belongs to superfamily DUF881. Contains probable N-terminal signal sequence. P71582 No cell wall and cell processes ML0014 MMAR_0014 MSMEG_0027 Mb0012 NC_000962.3 Mycobrowser_v2 CDS 250120 251598 . + 0 Rv0210 Rv0210 Unknown Hypothetical protein Rv0210, (MTCY08D5.05), len: 492 aa. Hypothetical unknown protein. Possibly membrane protein; has hydrophobic stretches around aa 333 - 381. P96392 No conserved hypotheticals ML2623 MMAR_0450 MSMEG_0254 Mb0216 NC_000962.3 Mycobrowser_v2 CDS 105324 106715 . + 0 Rv0096 PPE1 Function unknown PPE family protein PPE1 Rv0096, (MTCY251.15), len: 463 aa. PPE1, Member of the Mycobacterium tuberculosis PPE family, similar to many. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Q10892 No PE/PPE ML1991 MMAR_0261 Mb0099 NC_000962.3 Mycobrowser_v2 CDS 25913 26881 . - 0 Rv0021c Rv0021c Function unknown Conserved hypothetical protein Rv0021c, (MTCY10H4.21c), len: 322 aa. Conserved hypothetical protein, similar to various proteins. P71591 No conserved hypotheticals MMAR_1721 Mb0021c NC_000962.3 Mycobrowser_v2 CDS 1 1524 . + 0 Rv0001 dnaA Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (DNAA box): 5'-TTATC(C/A)A(C/A)A-3'. DNAA binds to ATP and to acidic phospholipids. DNAA protein binds the origin of replication (oriC), ATP and ADP, and exhibited weak ATPase activity. Chromosomal replication initiator protein DnaA Rv0001, (MT0001, MTV029.01, P49993), len: 507 aa. dnaA, chromosomal replication initiator protein (see citations below), equivalent to other Mycobacterial chromosomal replication initiator proteins. Also highly similar to others except in N-terminus e.g. Q9ZH75|DNAA_STRCH chromosomal replication initiator protein from Streptomyces chrysomallus (624 aa). Contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS01008 DnaA protein signature. Belongs to the DnaA family. Note that the first base of this gene has been taken as base 1 of the Mycobacterium tuberculosis H37Rv genomic sequence. P49993 No information pathways ML0001 MMAR_0001 MSMEG_6947 Mb0001 NC_000962.3 Mycobrowser_v2 CDS 2386293 2387171 . + 0 Rv2125 Rv2125 Unknown Conserved hypothetical protein Rv2125, (MTCY261.21), len: 292 aa. Conserved hypothetical protein, similar to many. O33260 No conserved hypotheticals ML1306 MMAR_3107 MSMEG_4186 Mb2149 NC_000962.3 Mycobrowser_v2 CDS 2052 3260 . + 0 Rv0002 dnaN DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP-independent) along duplex DNA [catalytic activity: N deoxynucleoside triphosphate = N diphosphate + {DNA}N]. DNA polymerase III (beta chain) DnaN (DNA nucleotidyltransferase) Rv0002, (MTV029.02, MTCY10H4.0), len: 402 aa. DnaN, DNA polymerase III (beta chain) (see citations below), equivalent to other Mycobacterial DNA polymerases III beta chain. Also highly similar to others e.g. P27903|DP3B_STRCO DNA polymerase III beta chain from Streptomyces coelicolor (376 aa). Overlaps and extends CDS in neighbouring cosmid MTCY10H4.01. Q50790 No information pathways ML0002 MMAR_0002 MSMEG_0001 Mb0002 NC_000962.3 Mycobrowser_v2 CDS 3280 4437 . + 0 Rv0003 recF The RECF protein is involved in DNA metabolism and recombination; it is required for DNA replication and normal sos inducibility. RECF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP. DNA replication and repair protein RecF (single-strand DNA binding protein) Rv0003, (MTCY10H4.01), len: 385 aa. RecF, DNA replication and repair protein (see citations below), equivalent to other mycobacterial DNA replication and repair proteins. Also highly similar to many others. Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00617 RecF protein signature 1, and PS00618 RecF protein signature 2. Belongs to the RecF family. Q59586 No information pathways ML0003 MMAR_0003 MSMEG_0003 Mb0003 NC_000962.3 Mycobrowser_v2 CDS 4434 4997 . + 0 Rv0004 Rv0004 Function unknown Conserved hypothetical protein Rv0004, (MTCY10H4.02), len: 187 aa. Conserved hypothetical protein (see Salazar et al., 1996). Belongs to superfamily DUF721; this family contains several actinomycete proteins of unknown function. P71573 No conserved hypotheticals ML0004 MMAR_0004 MSMEG_0004 Mb0004 NC_000962.3 Mycobrowser_v2 CDS 5240 7267 . + 0 Rv0005 gyrB DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [catalytic activity: ATP-dependent breakage, passage and rejoining of double-stranded DNA]. DNA gyrase (subunit B) GyrB (DNA topoisomerase (ATP-hydrolysing)) (DNA topoisomerase II) (type II DNA topoisomerase) Rv0005, (MTCY10H4.03), len: 675 aa. GyrB, DNA gyrase subunit B (see citations below). Contains PS00177 DNA topoisomerase II signature. Belongs to the type II topoisomerase family. Start changed since first submission (-39 aa). P0C5C5 No information pathways ML0005 MMAR_0005 MSMEG_0005 Mb0005 NC_000962.3 Mycobrowser_v2 CDS 7302 9818 . + 0 Rv0006 gyrA DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [catalytic activity: ATP-dependent breakage, passage and rejoining of double-stranded DNA]. DNA gyrase (subunit A) GyrA (DNA topoisomerase (ATP-hydrolysing)) (DNA topoisomerase II) (type II DNA topoisomerase) Rv0006, (MTCY10H4.04), len: 838 aa. GyrA, DNA gyrase subunit A (see citations below). Contains PS00018 EF-hand calcium-binding domain. Q07702 No information pathways ML0006 MMAR_0006 MSMEG_0006 Mb0006 NC_000962.3 Mycobrowser_v2 CDS 14914 15612 . + 0 Rv0013 trpG Possibly involved in biosynthesis of tryptophan (at the first step). Supposed tetramer of two components I and two components II: component I (Rv1609|trpE) catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II (Rv0013|trpG) provides glutamine amidotransferase activity. Possibly participates in the tryptophan-dependent indole-3-acetic acid production [catalytic activity: chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate]. Possible anthranilate synthase component II TrpG (glutamine amidotransferase) Rv0013, (MTCY10H4.13), len: 232 aa. Possible trpG, anthranilate synthase component II (glutamine amidotransferase). Contains PS00606 Beta-ketoacyl synthases active site; and PS00442 Glutamine amidotransferases class-I active site. Similarity to other type-1 glutamine amidotransferase domains. Note that previously known as pabA. Q7DAK6 No intermediary metabolism and respiration ML0015 MMAR_0015 MSMEG_0029 Mb0013 NC_000962.3 Mycobrowser_v2 CDS 23861 25444 . - 0 Rv0020c fhaA Signal transduction Conserved protein with FHA domain, FhaA Rv0020c, (MTCY10H4.20c), len: 527 aa. FhaA, TB39.8, conserved protein with forkhead-associated domain (IPR000253) at C-terminus, may be involved in signal transduction. Alternative start codon in position 24979 has been suggested (see citation below). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P71590 No regulatory proteins ML0022 MMAR_0022 MSMEG_0035 Mb0020c NC_000962.3 Mycobrowser_v2 tRNA 25644 25726 . + 0 MTB000003 leuT - tRNA-Leu (CAG) leuT, tRNA-Leu, anticodon cag, length = 83 No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 15590 17470 . - 0 Rv0014c pknB Involved in signal transduction (via phosphorylation). Thought to regulate cell division/differentiation. Can phosphorylate the peptide substrate myelin basic protein (MBP) [catalytic activity: ATP + a protein = ADP + a phosphoprotein]. Transmembrane serine/threonine-protein kinase B PknB (protein kinase B) (STPK B) Rv0014c, (MTCY10H4.14c), len: 626 aa. PknB, transmembrane serine/threonine-protein kinase (see citations below). Contains PS00107 Protein kinases ATP-binding region signature, and PS00108 Serine/Threonine protein kinases active-site signature. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases. Experimental studies show evidence of auto-phosphorylation on serine/threonine residues. PknB has been shown to be a substrate for PstP and its kinase activity is affected by PstP-mediated dephosphorylation. PknB and PstP (Rv0018c) may act as a functional pair in vivo to control mycobacterial cell growth. P0A5S4 No regulatory proteins ML0016 MMAR_0016 MSMEG_0028 Mb0014c NC_000962.3 Mycobrowser_v2 CDS 27595 28365 . + 0 Rv0023 Rv0023 Possibly involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0023, (MTCY10H4.23), len: 256 aa. Possible transcriptional regulator. Contains probable helix-turn helix motif from aa 19 to 40 (Score 1615, +4.69 SD). P67704 No regulatory proteins MMAR_0042 Mb0023 NC_000962.3 Mycobrowser_v2 CDS 1176011 1176286 . - 0 Rv1053c Rv1053c Unknown Hypothetical protein Rv1053c, (MTV017.06c), len: 91 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53401 No conserved hypotheticals Mb1082c NC_000962.3 Mycobrowser_v2 CDS 29245 29607 . + 0 Rv0025 Rv0025 Function unknown Conserved hypothetical protein Rv0025, (MTCY10H4.25), len: 120 aa. Conserved hypothetical protein, showing some similarity to other proteins from Mycobacterium tuberculosis e.g. Rv0739 (268 aa), FASTA score: (37.6% identity in 101 aa overlap), and Rv0026 FASTA score: (35.4% identity in 113 aa overlap); etc. P71595 No conserved hypotheticals MMAR_0044 Mb0026 NC_000962.3 Mycobrowser_v2 CDS 17467 18762 . - 0 Rv0015c pknA Involved in signal transduction (via phosphorylation). Thought to regulate morphological changes associated with cell division/differentiation process. Phosphorylates at serine and threonine residues [catalytic activity: ATP + a protein = ADP + a phosphoprotein]. Transmembrane serine/threonine-protein kinase A PknA (protein kinase A) (STPK A) Rv0015c, (MTCY10H4.15c), len: 431 aa. PknA, transmembrane serine/threonine-protein kinase, magnesium/manganese dependent (see citations below). Contains PS00108 Serine/Threonine protein kinases active-site signature. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases. It has been shown that sodium orthovanadate inhibits the activity of the enzyme in vitro. P65726 No regulatory proteins ML0017 MMAR_0017 MSMEG_0030 Mb0015c NC_000962.3 Mycobrowser_v2 CDS 18759 20234 . - 0 Rv0016c pbpA Involved in peptidoglycan synthesis (at the final stages). Cell wall formation; PBPA is supposed to be responsible for the determination of the rod shape of the cell. It synthesizes cross-linked peptidoglycan from lipid intermediates. Probable penicillin-binding protein PbpA Rv0016c, (MTCY10H4.16c), len: 491 aa. Probable pbpA, penicillin-binding protein, similar to many. P71586 No cell wall and cell processes ML0018 MMAR_0018 MSMEG_0031 Mb0016c NC_000962.3 Mycobrowser_v2 CDS 20231 21640 . - 0 Rv0017c rodA This is a septum-peptidoglycan biosynthetic protein, involved in cell wall formation. Plays a role in the stabilization of the FTSZ ring during cell division. Probable cell division protein RodA Rv0017c, (MTCY10H4.17c), len: 469 aa. Probable rodA (alternate gene name: ftsW), cell division protein, integral membrane protein. Belongs to the FTSW/RODA/SPOVE family. P63760 No cell wall and cell processes ML0019 MMAR_0019 MSMEG_0032 Mb0017c NC_000962.3 Mycobrowser_v2 CDS 21637 23181 . - 0 Rv0018c pstP Involved in regulation (using dephosphorylation of a specific phosphorylated substrate). Phosphoserine/threonine phosphatase PstP Rv0018c, (MTCY10H4.18c), len: 514 aa. PstP, phosphoserine/threonine phosphatase. Experimental studies have shown that PstP specifically dephosporylates model phospho-Ser/Thr substrates and it is likely that PknB (Rv0014c) and PstP may act as a functional pair in vivo to control mycobacterial cell growth (See Boitel et al., 2003). P71588 No regulatory proteins ML0020 MMAR_0020 MSMEG_0033 Mb0018c NC_000962.3 Mycobrowser_v2 CDS 23270 23737 . - 0 Rv0019c fhaB Signal transduction Conserved protein with FHA domain, FhaB Rv0019c, (MTCY10H4.19c), len: 155 aa. FhaB, conserved protein with forkhead-associated domain (IPR000253), probably involved in signal transduction. P71589 No regulatory proteins ML0021 MMAR_0021 MSMEG_0034 Mb0019c NC_000962.3 Mycobrowser_v2 CDS 27023 27442 . - 0 Rv0022c whiB5 Involved in transcriptional mechanism. Probable transcriptional regulatory protein WhiB-like WhiB5 Rv0022c, (MTCY10H4.22c), len: 139 aa. Probable whiB5 (alternate gene name: whmG), WhiB-like regulatory protein (see citations below), similar to WhiB paralogue of Streptomyces coelicolor, wblE gene product (85 aa). P71592 No regulatory proteins MMAR_0041 Mb0022c NC_000962.3 Mycobrowser_v2 CDS 59122 59376 . + 0 Rv0055 rpsR1 This protein has been implicated in aminoacyl-transfer RNA binding. It appears to be situated at the decoding site of messenger RNA. 30S ribosomal protein S18-1 RpsR1 Rv0055, (MTCY21D4.18), len: 84 aa. rpsR1, 30S ribosomal protein S18-1. Belongs to the S18P family of ribosomal proteins. Note that previously known as rpsR. P69230 No information pathways ML2683 MMAR_0074 MSMEG_6895 Mb0056 NC_000962.3 Mycobrowser_v2 CDS 34295 36610 . + 0 Rv0032 bioF2 Could be involved in biotin biosynthesis (at the first step) [catalytic activity: 6-carboxyhexanoyl-CoA + L-alanine = 8-amino-7-oxononanoate + CoA + CO2]. Possible 8-amino-7-oxononanoate synthase BioF2 (AONS) (8-amino-7-ketopelargonate synthase) (7-keto-8-amino-pelargonic acid synthetase) (7-KAP synthetase) (L-alanine--pimelyl CoA ligase) Rv0032, (MTCY10H4.32), len: 771 aa. Probable bioF2, 8-amino-7-oxononanoate synthase, with its C-terminal similar to others. Contains PS00599 Aminotransferases class-II pyridoxal-phosphate attachment site. Belongs to class-II of pyridoxal-phosphate-dependent aminotransferases. P71602 No intermediary metabolism and respiration Mb0033 NC_000962.3 Mycobrowser_v2 RBS 58173 58178 . + 0 Rv0053 rpsF Binds together with S18 to 16S ribosomal RNA. 30S ribosomal protein S6 RpsF Rv0053, (MTCY21D4.16), len: 96 aa. rpsF, 30S ribosomal protein S6, highly similar to many. Contains PS01048 Ribosomal protein S6 signature. Belongs to the S6P family of ribosomal proteins. P66591 No information pathways ML2685 MMAR_0072 MSMEG_6897 Mb0054 NC_000962.3 Mycobrowser_v2 CDS 58192 58482 . + 0 Rv0053 rpsF Binds together with S18 to 16S ribosomal RNA. 30S ribosomal protein S6 RpsF Rv0053, (MTCY21D4.16), len: 96 aa. rpsF, 30S ribosomal protein S6, highly similar to many. Contains PS01048 Ribosomal protein S6 signature. Belongs to the S6P family of ribosomal proteins. P66591 No information pathways ML2685 MMAR_0072 MSMEG_6897 Mb0054 NC_000962.3 Mycobrowser_v2 CDS 36607 36870 . + 0 Rv0033 acpA Key component in de novo fatty acid biosynthesis. This protein is supposed to be the carrier of the growing fatty acid chain in fatty acid biosynthesis. Probable acyl carrier protein AcpA (ACP) Rv0033, (MTCY10H4.33), len: 87 aa. Probable acpA (alternate gene name: acpP), acyl carrier protein, similar to others. Also similar to proteins of Mycobacterium tuberculosis Rv1344 and Rv2244 (31.5% identity in 73 aa overlap). P71603 No lipid metabolism Mb0034 NC_000962.3 Mycobrowser_v2 CDS 36867 37262 . + 0 Rv0034 Rv0034 Function unknown Conserved hypothetical protein Rv0034, (MTCY10H4.34), len: 131 aa. Conserved hypothetical protein, showing weak similarity to AE001980|AE001980_7 hypothetical protein from Deinococcus radiodurans (120 aa), FASTA scores: opt: 141, E(): 0.0028, (29.3% identity in 123 aa overlap). P64673 No conserved hypotheticals Mb0035 NC_000962.3 Mycobrowser_v2 ncRNA 1471619 1471742 . + 0 MTB000064 mcr3 Unknown Putative small regulatory RNA mcr3, putative small regulatory RNA (See DiChiara et al., 2010). 5'-end mapped by 5'RLM-RACE in M. bovis BGC Pasteur, 3'-end not mapped. No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 37259 38947 . + 0 Rv0035 fadD34 Function unknown, but involved in lipid degradation. Probable fatty-acid-CoA ligase FadD34 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv0035, (MTCY10H4.35), len: 562 aa. Probable fadD34, fatty-acid-CoA synthetase, similar to many. Q7DAJ8 No lipid metabolism MMAR_4970 Mb0036 NC_000962.3 Mycobrowser_v2 CDS 39056 39829 . - 0 Rv0036c Rv0036c Function unknown Conserved protein Rv0036c, (MTCY10H4.36c), len: 257 aa. Conserved protein, highly similar to CAB95889.1|AL359988 conserved hypothetical protein from Streptomyces (276 aa). Also some similarity to Rv3099c|MTCY164_10 (283 aa), FASTA scores: E(): 3.3e-05, (25.9% identity in 205 aa overlap). P64675 No conserved hypotheticals MMAR_0051 MSMEG_6923 Mb0037c NC_000962.3 Mycobrowser_v2 CDS 39877 41202 . - 0 Rv0037c Rv0037c Unknown; possibly involved in transport of macrolide across the membrane. Probable conserved integral membrane protein Rv0037c, (MTCY10H4.37c), len: 441 aa. Probable conserved integral membrane protein, member of major facilitator superfamily (MFS) possibly involved in transport of macrolide. P0A5C1 No cell wall and cell processes ML0027 MMAR_0052 MSMEG_6922 Mb0038c NC_000962.3 Mycobrowser_v2 CDS 43562 46471 . + 0 Rv0041 leuS Involved in translation mechanism [catalytic activity: ATP + L-leucine + tRNA(LEU) = AMP + diphosphate + L-leucyl-tRNA(LEU)]. Probable leucyl-tRNA synthetase LeuS (leucine--tRNA ligase) (LEURS) Rv0041, (MTCY21D4.04), len: 969 aa. Probable leucyl-tRNA synthetase, similar to many. Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Belongs to class-I aminoacyl-tRNA synthetase family. P67510 No information pathways ML0032 MMAR_0057 MSMEG_6917 Mb0042 NC_000962.3 Mycobrowser_v2 CDS 46581 47207 . - 0 Rv0042c Rv0042c Possibly involved in transcriptional mechanism. Possible transcriptional regulatory protein (probably MarR-family) Rv0042c, (MTCY21D4.05c), len: 208 aa. Possible transcriptional regulatory protein, MarR-family. Some similarity to Mycobacterium tuberculosis proteins Rv2327, Rv0880, and Rv1404. P71699 No regulatory proteins ML2696 MMAR_0059 MSMEG_6913 Mb0043c NC_000962.3 Mycobrowser_v2 CDS 47366 48100 . - 0 Rv0043c Rv0043c Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably GntR-family) Rv0043c, (MTCY21D4.06c), len: 244 aa. Probable transcriptional regulator, GntR family, similar to others. P67737 No regulatory proteins MMAR_0062 MSMEG_6908 Mb0044c NC_000962.3 Mycobrowser_v2 CDS 48233 49027 . - 0 Rv0044c Rv0044c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0044c, (MTCY21D4.07c), len: 264 aa. Possible oxidoreductase, highly similar to AAD32732.1|MmcI|AF127374| F420-dependent H4MPT reductase from Streptomyces lavendulae (264 aa). Also similar to Mycobacterium tuberculosis proteins e.g. Rv1855c, Rv0953c, Rv0791c, Rv0132c, etc. P71701 No intermediary metabolism and respiration ML2694 MMAR_0063 MSMEG_6907 Mb0045c NC_000962.3 Mycobrowser_v2 CDS 49043 49939 . - 0 Rv0045c Rv0045c Function unknown; probably involved in lipid biosynthesis. Possible hydrolase Rv0045c, (MTCY21D4.08c), len: 298 aa. Possible hydrolase, showing similarity with others. Also similar to Mycobacterium tuberculosis proteins Rv3473c, Rv1123c, Rv1938, Rv3617, Rv3670, etc. P71702 No lipid metabolism ML2693 MMAR_0064 MSMEG_6906 Mb0046c NC_000962.3 Mycobrowser_v2 CDS 50021 51124 . - 0 Rv0046c ino1 Involved in phosphatidylinositol (PI) biosynthetic pathway [catalytic activity: D-glucose 6-phosphate = 1L-myo-inositol 1-phosphate]. myo-inositol-1-phosphate synthase Ino1 (inositol 1-phosphate synthetase) (D-glucose 6-phosphate cycloaldolase) (glucose 6-phosphate cyclase) (glucocycloaldolase) Rv0046c, (MTCY21D4.09c), len: 367 aa. Ino1 (alternate gene name: tbINO), myo-inositol-1-phosphate synthase (see citations below). P71703 No intermediary metabolism and respiration ML0396,ML2692 MMAR_0065 MSMEG_6904 Mb0047c NC_000962.3 Mycobrowser_v2 RBS 51132 51138 . - 0 Rv0046c ino1 Involved in phosphatidylinositol (PI) biosynthetic pathway [catalytic activity: D-glucose 6-phosphate = 1L-myo-inositol 1-phosphate]. myo-inositol-1-phosphate synthase Ino1 (inositol 1-phosphate synthetase) (D-glucose 6-phosphate cycloaldolase) (glucose 6-phosphate cyclase) (glucocycloaldolase) Rv0046c, (MTCY21D4.09c), len: 367 aa. Ino1 (alternate gene name: tbINO), myo-inositol-1-phosphate synthase (see citations below). P71703 No intermediary metabolism and respiration ML0396,ML2692 MMAR_0065 MSMEG_6904 Mb0047c NC_000962.3 Mycobrowser_v2 CDS 51185 51727 . - 0 Rv0047c Rv0047c Function unknown Conserved protein Rv0047c, (MTCY21D4.10c), len: 180 aa. Conserved protein, similar to many. P71704 No conserved hypotheticals ML2691 MMAR_0066 MSMEG_6903 Mb0048c NC_000962.3 Mycobrowser_v2 RBS 51735 51741 . - 0 Rv0047c Rv0047c Function unknown Conserved protein Rv0047c, (MTCY21D4.10c), len: 180 aa. Conserved protein, similar to many. P71704 No conserved hypotheticals ML2691 MMAR_0066 MSMEG_6903 Mb0048c NC_000962.3 Mycobrowser_v2 CDS 51828 52697 . - 0 Rv0048c Rv0048c Unknown Possible membrane protein Rv0048c, MTCY21D4.11c, len: 289 aa. Possible membrane protein. P71705 No cell wall and cell processes ML2690 MMAR_0067 MSMEG_6902 Mb0049c NC_000962.3 Mycobrowser_v2 CDS 53663 55699 . + 0 Rv0050 ponA1 Involved in peptidoglycan synthesis (at the final stages), cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits). Probable bifunctional penicillin-binding protein 1A/1B PonA1 (murein polymerase) (PBP1): penicillin-insensitive transglycosylase (peptidoglycan TGASE) + penicillin-sensitive transpeptidase (DD-transpeptidase) Rv0050, (MTCY21D4.13), len: 678 aa. Probable ponA1, penicillin-binding protein (class A), bienzymatic protein with transglycosylase and transpeptidase activities (see Graham & Clark-Curtiss 1999), highly similar to many (see Billman-Jacobe et al., 1999). Belongs to the transglycosylase family in the N-terminal section, and to the transpeptidase family in the C-terminal section. P71707 No cell wall and cell processes ML2688 MMAR_0069 MSMEG_6900 Mb0051 NC_000962.3 Mycobrowser_v2 CDS 55696 57378 . + 0 Rv0051 Rv0051 Unknown Probable conserved transmembrane protein Rv0051, (MTCY21D4.14), len:560 aa. Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). Probable conserved transmembrane protein. P71708 No cell wall and cell processes ML2687 MMAR_0070 MSMEG_6899 Mb0052 NC_000962.3 Mycobrowser_v2 RBS 57395 57398 . + 0 Rv0052 Rv0052 Function unknown Conserved protein Rv0052, (MTCY21D4.15), len: 187 aa. Conserved protein, similar to others including Rv1930c from Mycobacterium tuberculosis (174 aa). May be a membrane protein. P71709 No conserved hypotheticals ML2686 MMAR_0071 Mb0053 NC_000962.3 Mycobrowser_v2 CDS 57410 57973 . + 0 Rv0052 Rv0052 Function unknown Conserved protein Rv0052, (MTCY21D4.15), len: 187 aa. Conserved protein, similar to others including Rv1930c from Mycobacterium tuberculosis (174 aa). May be a membrane protein. P71709 No conserved hypotheticals ML2686 MMAR_0071 Mb0053 NC_000962.3 Mycobrowser_v2 RBS 58575 58582 . + 0 Rv0054 ssb This protein is essential for replication of the chromosome. It is also involved in DNA recombination and repair. Single-strand binding protein Ssb (helix-destabilizing protein) Rv0054, (MTCY21D4.17), len: 164 aa. ssb, single-strand binding protein (see Mizrahi & Andersen 1998), highly similar to others. Belongs to the SSB family. P0A610 No information pathways ML2684 MMAR_0073 MSMEG_6896 Mb0055 NC_000962.3 Mycobrowser_v2 CDS 58586 59080 . + 0 Rv0054 ssb This protein is essential for replication of the chromosome. It is also involved in DNA recombination and repair. Single-strand binding protein Ssb (helix-destabilizing protein) Rv0054, (MTCY21D4.17), len: 164 aa. ssb, single-strand binding protein (see Mizrahi & Andersen 1998), highly similar to others. Belongs to the SSB family. P0A610 No information pathways ML2684 MMAR_0073 MSMEG_6896 Mb0055 NC_000962.3 Mycobrowser_v2 RBS 59390 59395 . + 0 Rv0056 rplI Binds to the 23S rRNA. 50S ribosomal protein L9 RplI Rv0056, (MTCY21D4.19), len: 152 aa. rplI, 50S ribosomal protein L9. Contains PS00651 Ribosomal protein L9 signature. Belongs to the L9P family of ribosomal proteins. P66315 No information pathways ML2682 MMAR_0075 MSMEG_6894 Mb0057 NC_000962.3 Mycobrowser_v2 CDS 59409 59867 . + 0 Rv0056 rplI Binds to the 23S rRNA. 50S ribosomal protein L9 RplI Rv0056, (MTCY21D4.19), len: 152 aa. rplI, 50S ribosomal protein L9. Contains PS00651 Ribosomal protein L9 signature. Belongs to the L9P family of ribosomal proteins. P66315 No information pathways ML2682 MMAR_0075 MSMEG_6894 Mb0057 NC_000962.3 Mycobrowser_v2 CDS 59896 60417 . + 0 Rv0057 Rv0057 Unknown Hypothetical protein Rv0057, (MTCY21D4.20), len: 173 aa. Hypothetical unknown protein. P64679 No conserved hypotheticals ML2681 Mb0058 NC_000962.3 Mycobrowser_v2 RBS 60381 60386 . + 0 Rv0058 dnaB Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity. The intein is an endonuclease (potential). Probable replicative DNA helicase DnaB Rv0058, (MTV030.01, MTCY21D4.21), len: 874 aa. Probable dnaB, replicative DNA helicase. Contains an intein (position 61630..62838) similar to, and in the same position as, those in Sycnechocystis and Rhodothermus marinus (see citation below) and C-terminal extein (position 62839..63015) similar to many dnaB proteins. This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation. Belongs to the helicase family, DNAB subfamily. In the intein section; belongs to the homing endonuclease family. P71715 No information pathways ML2680 MSMEG_6892 Mb0059 NC_000962.3 Mycobrowser_v2 CDS 60396 63020 . + 0 Rv0058 dnaB Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity. The intein is an endonuclease (potential). Probable replicative DNA helicase DnaB Rv0058, (MTV030.01, MTCY21D4.21), len: 874 aa. Probable dnaB, replicative DNA helicase. Contains an intein (position 61630..62838) similar to, and in the same position as, those in Sycnechocystis and Rhodothermus marinus (see citation below) and C-terminal extein (position 62839..63015) similar to many dnaB proteins. This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation. Belongs to the helicase family, DNAB subfamily. In the intein section; belongs to the homing endonuclease family. P71715 No information pathways ML2680 MSMEG_6892 Mb0059 NC_000962.3 Mycobrowser_v2 CDS 63200 63892 . + 0 Rv0059 Rv0059 Unknown Hypothetical protein Rv0059, (MTV030.02), len: 230 aa. Hypothetical unknown protein. O53604 No conserved hypotheticals Mb0060 NC_000962.3 Mycobrowser_v2 CDS 65552 66694 . + 0 Rv0062 celA1 The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) exocellobiohydrolases that cut the dissaccharide cellobiose from the nonreducing end of the cellulose polymer chain; (3) beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose [catalytic activity:endohydrolysis of 1,4-beta-D-glucosidic linkages in cellulose]. Possible cellulase CelA1 (endoglucanase) (endo-1,4-beta-glucanase) (FI-cmcase) (carboxymethyl cellulase) Rv0062, (MTV030.05), len: 380 aa. Possible celA1, cellulase, similar to many. Seems to belong to cellulase family B (family 6 of glycosyl hydrolases). Note that previously known as celA. Q79G13 No intermediary metabolism and respiration MMAR_0107 MSMEG_6752 Mb0063 NC_000962.3 Mycobrowser_v2 RBS 66912 66915 . + 0 Rv0063 Rv0063 Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0063, (MTV030.06), len: 479 aa. Possible oxidoreductase, similar to many. Similar to Mycobacterium tuberculosis proteins e.g. Rv3107c, Rv1257c, etc. Contains PS00862 Oxygen oxidoreductases covalent FAD-binding site. O53608 No intermediary metabolism and respiration MMAR_0110 Mb0064 NC_000962.3 Mycobrowser_v2 CDS 66923 68362 . + 0 Rv0063 Rv0063 Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0063, (MTV030.06), len: 479 aa. Possible oxidoreductase, similar to many. Similar to Mycobacterium tuberculosis proteins e.g. Rv3107c, Rv1257c, etc. Contains PS00862 Oxygen oxidoreductases covalent FAD-binding site. O53608 No intermediary metabolism and respiration MMAR_0110 Mb0064 NC_000962.3 Mycobrowser_v2 CDS 89924 90403 . + 0 Rv0082 Rv0082 Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv0082, (MTV030.26), len: 159 aa. Probable oxidoreductase, highly similar or similar to other various oxidoreductases. Nucleotide position 90144 in the genome sequence has been corrected, A:G resulting in Q74R. O53627 No intermediary metabolism and respiration MMAR_1654 Mb0085 NC_000962.3 Mycobrowser_v2 RBS 68609 68614 . + 0 Rv0064 Rv0064 Unknown Probable conserved transmembrane protein Rv0064, (MTV030.07), len: 979 aa. Probable conserved transmembrane protein, similar to many. Contains probable coiled-coil domain from aa 948 to 976. O53609 No cell wall and cell processes Mb0065 NC_000962.3 Mycobrowser_v2 CDS 68620 71559 . + 0 Rv0064 Rv0064 Unknown Probable conserved transmembrane protein Rv0064, (MTV030.07), len: 979 aa. Probable conserved transmembrane protein, similar to many. Contains probable coiled-coil domain from aa 948 to 976. O53609 No cell wall and cell processes Mb0065 NC_000962.3 Mycobrowser_v2 rRNA 1473658 1476795 . + 0 MTB000020 rrl - Ribosomal RNA 23S rrl, 23S rRNA gene (approximate coordinates). No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 71589 71828 . + 0 Rv0064A vapB1 Unknown Possible antitoxin VapB1 Rv0064A, len: 79 aa. Possible vapB1, antitoxin, part of toxin-antitoxin (TA) operon with Rv0065 (See Arcus et al., 2005; Pandey and Gerdes, 2005). Weakly similar to others in Mycobacterium tuberculosis e.g. Rv0300 (73 aa), Rv1721c (75 aa) P0CW29 No virulence, detoxification, adaptation NC_000962.3 Mycobrowser_v2 RBS 71810 71813 . + 0 Rv0065 vapC1 Unknown Possible toxin VapC1 Rv0065, (MTV030.08), len: 133 aa. Possible vapC1, toxin, part of toxin-antitoxin (TA) operon with Rv0064A, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to several others in Mycobacterium tuberculosis: Rv0960 (127 aa), Rv1720c (129 aa), and Rv0549c (137 aa). O53610 No virulence, detoxification, adaptation Mb0066 NC_000962.3 Mycobrowser_v2 CDS 71821 72222 . + 0 Rv0065 vapC1 Unknown Possible toxin VapC1 Rv0065, (MTV030.08), len: 133 aa. Possible vapC1, toxin, part of toxin-antitoxin (TA) operon with Rv0064A, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to several others in Mycobacterium tuberculosis: Rv0960 (127 aa), Rv1720c (129 aa), and Rv0549c (137 aa). O53610 No virulence, detoxification, adaptation Mb0066 NC_000962.3 Mycobrowser_v2 RBS 89565 89569 . + 0 Rv0081 Rv0081 Involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv0081, (MTV030.25), len: 114 aa. Probable transcriptional regulator, highly similar to others. O53626 No regulatory proteins MMAR_1653 MSMEG_6451 Mb0084 NC_000962.3 Mycobrowser_v2 CDS 89575 89919 . + 0 Rv0081 Rv0081 Involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv0081, (MTV030.25), len: 114 aa. Probable transcriptional regulator, highly similar to others. O53626 No regulatory proteins MMAR_1653 MSMEG_6451 Mb0084 NC_000962.3 Mycobrowser_v2 CDS 72274 74511 . - 0 Rv0066c icd2 Involved in the KREBS cycle [catalytic activity: isocitrate + NADP+ = 2-oxoglutarate + CO(2) + NADPH]. Probable isocitrate dehydrogenase [NADP] Icd2 (oxalosuccinate decarboxylase) (IDH) (NADP+-specific ICDH) (IDP) Rv0066c, (MTV030.09c), len: 745 aa. Probable icd2, isocitrate dehydrogenase NADP-dependent. Belongs to the monomeric-type family of IDH. Note that in H37Rv, Rv0066c is named icd2 and Rv3339c is icd1 while in CDC1551 and Erdman strains, Rv0066c is icd1 and Rv3339c is icd2. O53611 No intermediary metabolism and respiration ML2672 MMAR_0158 MSMEG_1654 Mb0067c NC_000962.3 Mycobrowser_v2 RBS 74518 74521 . - 0 Rv0066c icd2 Involved in the KREBS cycle [catalytic activity: isocitrate + NADP+ = 2-oxoglutarate + CO(2) + NADPH]. Probable isocitrate dehydrogenase [NADP] Icd2 (oxalosuccinate decarboxylase) (IDH) (NADP+-specific ICDH) (IDP) Rv0066c, (MTV030.09c), len: 745 aa. Probable icd2, isocitrate dehydrogenase NADP-dependent. Belongs to the monomeric-type family of IDH. Note that in H37Rv, Rv0066c is named icd2 and Rv3339c is icd1 while in CDC1551 and Erdman strains, Rv0066c is icd1 and Rv3339c is icd2. O53611 No intermediary metabolism and respiration ML2672 MMAR_0158 MSMEG_1654 Mb0067c NC_000962.3 Mycobrowser_v2 CDS 74629 75198 . - 0 Rv0067c Rv0067c Possibly involved in transcriptional mechanism. Possible transcriptional regulatory protein (possibly TetR-family) Rv0067c, (MTV030.10c), len: 189 aa. Possible transcriptional regulator, highly similar to many. Contains probable helix-turn-helix motif from aa 34 to 55 (Score 1523, +4.37 SD). O53612 No regulatory proteins ML0316 MSMEG_0878 Mb0068c NC_000962.3 Mycobrowser_v2 RBS 75291 75295 . + 0 Rv0068 Rv0068 Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv0068, (MTV030.11), len: 303 aa. Probable oxidoreductase, similar to many. O53613 No intermediary metabolism and respiration ML0315 MSMEG_0876 Mb0069 NC_000962.3 Mycobrowser_v2 CDS 75301 76212 . + 0 Rv0068 Rv0068 Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv0068, (MTV030.11), len: 303 aa. Probable oxidoreductase, similar to many. O53613 No intermediary metabolism and respiration ML0315 MSMEG_0876 Mb0069 NC_000962.3 Mycobrowser_v2 CDS 76237 77622 . - 0 Rv0069c sdaA Involved in gluconeogenesis from serine [catalytic activity: L-serine + H2O = pyruvate + NH3 + H2O]. Probable L-serine dehydratase SdaA (L-serine deaminase) (SDH) (L-SD) Rv0069c, (MTV030.12c), len: 461 aa. Probable sdaA, L-serine dehydratase. Belongs to the iron-sulfur dependent L-serine dehydratase family. Cofactor: iron-sulfur (4FE-4S) (probable). P66773 No intermediary metabolism and respiration ML1755 MMAR_0207 Mb0070c NC_000962.3 Mycobrowser_v2 CDS 77619 78896 . - 0 Rv0070c glyA2 Key enzyme in the biosynthesis of purines, lipids, other components. Interconversion of serine and glycine [catalytic activity: 5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine]. Serine hydroxymethyltransferase GlyA2 (serine methylase 2) (SHMT 2) Rv0070c, (MTV030.13c), len: 425 aa. glyA2, serine hydroxymethyltransferase. Contains PS00096 Serine hydroxymethyltransferase pyridoxal-phosphate attachment site. Belongs to the ShmT family. Cofactor: pyridoxal phosphate. O53615 No intermediary metabolism and respiration MMAR_0208 Mb0071c NC_000962.3 Mycobrowser_v2 CDS 79486 80193 . + 0 Rv0071 Rv0071 Unknown Possible maturase Rv0071, (MTV030.14), len: 235 aa. Possible maturase, similar to many proteins of the group II intron maturase family. Contains 5 VDP repeats at N-terminus, these are also found in two Streptococcus plasmid hypothetical proteins Q52246|X17092 and Q54942|X66468. O53616 No information pathways Mb0072 NC_000962.3 Mycobrowser_v2 CDS 608059 608535 . - 0 Rv0516c Rv0516c May be involved in regulating sigma factor Possible anti-anti-sigma factor Rv0516c, (MTCY20G10.06c), len: 158 aa. Possible anti-anti-sigma factor, showing some similarity to Rv1365c|MTCY02B10_29 from Mycobacterium tuberculosis (128 aa), FASTA scores: E(): 0.0012, (27.4% identity in 124 aa overlap). O33361 No information pathways MSMEG_0586 Mb0529c NC_000962.3 Mycobrowser_v2 RBS 608542 608545 . - 0 Rv0516c Rv0516c May be involved in regulating sigma factor Possible anti-anti-sigma factor Rv0516c, (MTCY20G10.06c), len: 158 aa. Possible anti-anti-sigma factor, showing some similarity to Rv1365c|MTCY02B10_29 from Mycobacterium tuberculosis (128 aa), FASTA scores: E(): 0.0012, (27.4% identity in 124 aa overlap). O33361 No information pathways MSMEG_0586 Mb0529c NC_000962.3 Mycobrowser_v2 CDS 80624 81673 . + 0 Rv0072 Rv0072 Thought to be involved in active transport of glutamine across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable glutamine-transport transmembrane protein ABC transporter Rv0072, (MTV030.16), len: 349 aa. Probable glutamine-transport transmembrane protein ABC-transporter (see citation below). Note that supposed act with near ORF Rv0073|MTV030.17 ATP-binding protein ABC-transporter. O53617 No cell wall and cell processes MMAR_0215 Mb0073 NC_000962.3 Mycobrowser_v2 RBS 81662 81667 . + 0 Rv0073 Rv0073 Thought to be involved in active transport of glutamine across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable glutamine-transport ATP-binding protein ABC transporter Rv0073, (MTV030.17), len: 330 aa. Probable glutamine-transport ATP-binding protein ABC-transporter (see citation below), similar to many ATP-binding proteins. Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00211 ABC transporters family signature, and PS00889 Cyclic nucleotide-binding domain signature 2. Belongs to the ATP-binding transport protein family (ABC transporters). Note that supposed act with near ORF Rv0072|MTV030.16 transmembrane ABC-transporter. O53618 No cell wall and cell processes MMAR_0216 Mb0074,Mb0075 NC_000962.3 Mycobrowser_v2 CDS 81676 82668 . + 0 Rv0073 Rv0073 Thought to be involved in active transport of glutamine across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable glutamine-transport ATP-binding protein ABC transporter Rv0073, (MTV030.17), len: 330 aa. Probable glutamine-transport ATP-binding protein ABC-transporter (see citation below), similar to many ATP-binding proteins. Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00211 ABC transporters family signature, and PS00889 Cyclic nucleotide-binding domain signature 2. Belongs to the ATP-binding transport protein family (ABC transporters). Note that supposed act with near ORF Rv0072|MTV030.16 transmembrane ABC-transporter. O53618 No cell wall and cell processes MMAR_0216 Mb0074,Mb0075 NC_000962.3 Mycobrowser_v2 RBS 82733 82738 . + 0 Rv0074 Rv0074 Function unknown Conserved protein Rv0074, (MTV030.18), len: 411 aa. Conserved protein, similar to many. O53619 No conserved hypotheticals ML1110 MMAR_4746 MSMEG_0297,MSMEG_4842 Mb0076 NC_000962.3 Mycobrowser_v2 CDS 82748 83983 . + 0 Rv0074 Rv0074 Function unknown Conserved protein Rv0074, (MTV030.18), len: 411 aa. Conserved protein, similar to many. O53619 No conserved hypotheticals ML1110 MMAR_4746 MSMEG_0297,MSMEG_4842 Mb0076 NC_000962.3 Mycobrowser_v2 CDS 83996 85168 . + 0 Rv0075 Rv0075 Function unknown; probably involved in cellular metabolism. Probable aminotransferase Rv0075, (MTV030.19), len: 390 aa. Probable aminotransferase, similar to many class-II pyridoxal-phosphate-dependent aminotransferases (MALY/PATB subfamily). Also similar to other proteins from Mycobacterium tuberculosis e.g. Rv2294, Rv0858c, etc. O53620 No intermediary metabolism and respiration ML1108 Mb0077 NC_000962.3 Mycobrowser_v2 CDS 85183 85572 . - 0 Rv0076c Rv0076c Unknown Probable membrane protein Rv0076c, (MTV030.20c), len: 129 aa. Probable membrane protein, with membrane-spanning domain at C-terminus. O53621 No cell wall and cell processes MMAR_0219 MSMEG_0583 Mb0078c NC_000962.3 Mycobrowser_v2 CDS 85636 86466 . - 0 Rv0077c Rv0077c Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv0077c, (MTV030.21c), len: 276 aa. Possible oxidoreductase, weakly similar to others from Streptomyces. Also similar to MTCY05A6_35 and MTCY1A11_10 from Mycobacterium tuberculosis. And shows some similarity in part with AAL17935.1|AY054120 putative epoxide hydrolase from Mycobacterium smegmatis (203 aa). O53622 No intermediary metabolism and respiration ML2676 MSMEG_6763 Mb0079c NC_000962.3 Mycobrowser_v2 RBS 86473 86477 . - 0 Rv0077c Rv0077c Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv0077c, (MTV030.21c), len: 276 aa. Possible oxidoreductase, weakly similar to others from Streptomyces. Also similar to MTCY05A6_35 and MTCY1A11_10 from Mycobacterium tuberculosis. And shows some similarity in part with AAL17935.1|AY054120 putative epoxide hydrolase from Mycobacterium smegmatis (203 aa). O53622 No intermediary metabolism and respiration ML2676 MSMEG_6763 Mb0079c NC_000962.3 Mycobrowser_v2 CDS 86528 87133 . + 0 Rv0078 Rv0078 Possibly involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv0078, (MTV030.22), len: 201 aa. Probable transcriptional regulator. Contains probable helix-turn-helix motif from aa 35 to 56 (Score 1348, +3.78 SD). O53623 No regulatory proteins ML2677 MMAR_0224 Mb0080 NC_000962.3 Mycobrowser_v2 CDS 87208 87801 . - 0 Rv0078A Rv0078A Unknown Hypothetical protein Rv0078A, len: 197 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q8VKS0 No conserved hypotheticals Mb0081c NC_000962.3 Mycobrowser_v2 CDS 90400 92322 . + 0 Rv0083 Rv0083 Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv0083, (MTV030.27, MTCY251.01), len: 640 aa. Probable oxidoreductase, showing some similarity to other various oxidoreductases. Nucleotide position 91071 in the genome sequence has been corrected, T:C resulting in I224I. Q10880 No intermediary metabolism and respiration MMAR_1655 Mb0086 NC_000962.3 Mycobrowser_v2 CDS 92328 93278 . + 0 Rv0084 hycD Involved in hydrogen metabolism; FHL pathway. Possible formate hydrogenlyase HycD (FHL) Rv0084, (MTCY251.02), len: 316 aa. Possible hycD (alternate gene name: hevD), formate hydrogenlyase, integral membrane protein, similar to others. Belongs to the complex I subunit 1 family. Q10881 No intermediary metabolism and respiration MMAR_1656 Mb0087 NC_000962.3 Mycobrowser_v2 CDS 93289 93951 . + 0 Rv0085 hycP Involved in hydrogen metabolism. Possible hydrogenase HycP Rv0085, (MTCY251.03), len: 220 aa. Possible hycP, hydrogenase, integral membrane protein. Belongs to NADH-ubiquinone/plastoquinone oxidoreductase chain 4L superfamily. P64681 No intermediary metabolism and respiration MMAR_1657 Mb0088 NC_000962.3 Mycobrowser_v2 CDS 93951 95417 . + 0 Rv0086 hycQ Possibly involved in hydrogen metabolism. Possible hydrogenase HycQ Rv0086, (MTCY251.04), len: 488 aa. Possible hycQ, hydrogenase, integral membrane protein. Belongs to the NADH-Ubiquinone/plastoquinone (complex I) superfamily. Q10883 No intermediary metabolism and respiration MMAR_1658 Mb0089 NC_000962.3 Mycobrowser_v2 CDS 95414 96892 . + 0 Rv0087 hycE Involved in hydrogen metabolism; FHL pathway. Possible formate hydrogenase HycE (FHL) Rv0087, (MTCY251.05), len: 492 aa. Possible hycE (alternate gene name: hevE), formate hydrogenlyase, similar to others. Belongs to the complex I 49 kDa subunit family. Q10884 No intermediary metabolism and respiration MMAR_1659 Mb0090 NC_000962.3 Mycobrowser_v2 CDS 96927 97601 . + 0 Rv0088 Rv0088 Unknown Possible polyketide cyclase/dehydrase Rv0088, (MTCY251.06), len: 224 aa. Possible polyketide cyclase/dehydrase. Belongs to the SRPBCC ligand-binding domain superfamily. Predicted to be an outer membrane protein (See Song et al., 2008). P0A5C3 No lipid metabolism ML2652 MMAR_0234 Mb0091 NC_000962.3 Mycobrowser_v2 CDS 97758 98351 . + 0 Rv0089 Rv0089 Thought to cause methylation. Possible methyltransferase/methylase Rv0089, (MTCY251.07), len: 197 aa. Possible methyltransferase, showing some weak similarity to others. Also some similarity with many biotin biosynthesis proteins. Belongs to the methyltransferase superfamily. P65346 No intermediary metabolism and respiration MMAR_0244 MSMEG_4708 Mb0092 NC_000962.3 Mycobrowser_v2 CDS 329705 330325 . - 0 Rv0273c Rv0273c Could be involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0273c, (MTV035.01c), len: 206 aa (start uncertain). Possible transcriptional regulator, showing some similarity to hypothetical regulators from Mycobacterium tuberculosis e.g. P96222|Rv3855|MTCY01A6.13c (216 aa); O08377|Rv1534|MTCY07A7A.03 (225 aa), FASTA scores: opt: 123, E(): 3.2e-06, (28.5% identity in 172 aa overlap). O86342 No regulatory proteins ML2543 MMAR_0533 MSMEG_0606 Mb0279c NC_000962.3 Mycobrowser_v2 RBS 330333 330337 . - 0 Rv0273c Rv0273c Could be involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0273c, (MTV035.01c), len: 206 aa (start uncertain). Possible transcriptional regulator, showing some similarity to hypothetical regulators from Mycobacterium tuberculosis e.g. P96222|Rv3855|MTCY01A6.13c (216 aa); O08377|Rv1534|MTCY07A7A.03 (225 aa), FASTA scores: opt: 123, E(): 3.2e-06, (28.5% identity in 172 aa overlap). O86342 No regulatory proteins ML2543 MMAR_0533 MSMEG_0606 Mb0279c NC_000962.3 Mycobrowser_v2 CDS 99684 100451 . + 0 Rv0091 mtn Responsible for cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH) [catalytic activity 1: methylthioadenosine + H2O = adenine + 5-methylthio-D-ribose] [catalytic activity 2: S-adenosyl-L-homocysteine + H2O = adenine + S-D-ribosyl-L-homocysteine]. Probable bifunctional MTA/SAH nucleosidase Mtn: 5'-methylthioadenosine nucleosidase (methylthioadenosine methylthioribohydrolase) + S-adenosylhomocysteine nucleosidase (S-adenosyl-L-homocysteine homocysteinylribohydrolase) Rv0091, (MTCY251.10), len: 255 aa. Probable mtn (alternate gene name: pfs), methylthioadenosine/S-Adenosylhomocysteine nucleosidase (MTA/SAH nucleosidase), including 5'-methylthioadenosine nucleosidase and S-adenosylhomocysteine nucleosidase, similar to others. Belongs to the MTN family. P67656 No intermediary metabolism and respiration MMAR_0251 MSMEG_1753 Mb0094 NC_000962.3 Mycobrowser_v2 CDS 545375 545821 . - 0 Rv0455c Rv0455c Function unknown Conserved protein Rv0455c, (MTV037.19c), len: 148 aa. Conserved protein, equivalent to CAC31896.1|AL583925 possible secreted protein from Mycobacterium leprae (153 aa). Has hydrophobic stretch at N-terminus. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). O53740 No conserved hypotheticals ML2380 MSMEG_3494 Mb0463c NC_000962.3 Mycobrowser_v2 RBS 100572 100575 . + 0 Rv0092 ctpA Cation-transporting ATPase; possibly catalyzes the transport of a cation (possibly copper) with the hydrolyse of ATP [catalytic activity: ATP + H(2)O + cation(in) = ADP + phosphate + cation(out)]. Cation transporter P-type ATPase a CtpA Rv0092, (MTCY251.11), len: 761 aa. CtpA, cation-transporting P-type ATPase a (transmembrane protein), highly similar to many. Contains PS01047 Heavy-metal-associated domain, and PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases), subfamily IB. Q10876 No cell wall and cell processes ML1987 MMAR_0264 Mb0095 NC_000962.3 Mycobrowser_v2 CDS 100583 102868 . + 0 Rv0092 ctpA Cation-transporting ATPase; possibly catalyzes the transport of a cation (possibly copper) with the hydrolyse of ATP [catalytic activity: ATP + H(2)O + cation(in) = ADP + phosphate + cation(out)]. Cation transporter P-type ATPase a CtpA Rv0092, (MTCY251.11), len: 761 aa. CtpA, cation-transporting P-type ATPase a (transmembrane protein), highly similar to many. Contains PS01047 Heavy-metal-associated domain, and PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases), subfamily IB. Q10876 No cell wall and cell processes ML1987 MMAR_0264 Mb0095 NC_000962.3 Mycobrowser_v2 CDS 102815 103663 . - 0 Rv0093c Rv0093c Unknown Probable conserved membrane protein Rv0093c, (MTCY251.12c), len: 282 aa. Probable conserved membrane protein, equivalent only to CAC30943.1|AL583924 probable integral membrane protein from Mycobacterium leprae (237 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Q10890 No cell wall and cell processes ML1988 MMAR_0263 Mb0096c NC_000962.3 Mycobrowser_v2 CDS 103710 104663 . - 0 Rv0094c Rv0094c Function unknown Conserved hypothetical protein Rv0094c, (MTCY251.13c), len: 317 aa. Member of 13E12 repeat family, showing some similarity to U15187|MLU15187_7 from Mycobacterium leprae (94 aa), FASTA score: (49.4% identity in 79 aa overlap). Q50655 No insertion seqs and phages Mb0097c NC_000962.3 Mycobrowser_v2 CDS 104805 105215 . - 0 Rv0095c Rv0095c Function unknown Conserved hypothetical protein Rv0095c, (MTCY251.14c), len: 136 aa. Member of 13E12 repeat, also partially similar to AF0418|AF041819_8 from Mycobacterium bovis BCG (222 aa), FASTA score: (89.6% identity in 96 aa overlap). Q10891 No insertion seqs and phages Mb0098c NC_000962.3 Mycobrowser_v2 RBS 106718 106723 . + 0 Rv0097 Rv0097 Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0097, (MTCY251.16), len: 289 aa. Possible oxidoreductase, equivalent to NP_302343.1|NC_002677 putative oxidoreductase from Mycobacterium leprae (289 aa). Also highly similar to BAB69377.1|AB070955 putative oxidoreductase from Streptomyces avermitilis (296 aa). Contains PS00077 Cytochrome c oxidase subunit I, copper B binding region signature. P67755 No intermediary metabolism and respiration ML1992 MMAR_0260 Mb0100 NC_000962.3 Mycobrowser_v2 CDS 106734 107603 . + 0 Rv0097 Rv0097 Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0097, (MTCY251.16), len: 289 aa. Possible oxidoreductase, equivalent to NP_302343.1|NC_002677 putative oxidoreductase from Mycobacterium leprae (289 aa). Also highly similar to BAB69377.1|AB070955 putative oxidoreductase from Streptomyces avermitilis (296 aa). Contains PS00077 Cytochrome c oxidase subunit I, copper B binding region signature. P67755 No intermediary metabolism and respiration ML1992 MMAR_0260 Mb0100 NC_000962.3 Mycobrowser_v2 CDS 107600 108151 . + 0 Rv0098 fcoT Involved in fatty acid metabolism Probable fatty acyl CoA thioesterase type III FcoT Rv0098, (MTCY251.17), len: 183 aa. FcoT, long-chain fatty acyl CoA thioesterase type III (See Wang et al., 2007), equivalent to CAC30948.1|AL583924 from Mycobacterium leprae (183 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P64685 No lipid metabolism ML1993 MMAR_0259 Mb0101 NC_000962.3 Mycobrowser_v2 CDS 108156 109778 . + 0 Rv0099 fadD10 Function unknown, but involvement in lipid degradation. Possible fatty-acid-CoA ligase FadD10 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv0099, (MTCY251.18), len: 540 aa. Possible fadD10, fatty-acid-CoA synthetase, similar to many. Contains PS00455 putative AMP-binding domain signature. Contains IPR000873 AMP-dependent synthetase/ligase domain. Belongs to the ATP-dependent AMP-binding enzyme family. Q10878 No lipid metabolism ML1994 MMAR_0258 Mb0102 NC_000962.3 Mycobrowser_v2 CDS 884072 884800 . - 0 Rv0790c Rv0790c Unknown Hypothetical protein Rv0790c, (MTCY369.34c), len: 242 aa. Hypothetical unknown protein. P71843 No conserved hypotheticals ML2217 MMAR_2558 Mb0814c NC_000962.3 Mycobrowser_v2 CDS 109783 110019 . + 0 Rv0100 Rv0100 Function unknown Conserved hypothetical protein Rv0100, (MTCY251.19), len: 78 aa. Conserved hypothetical protein, equivalent only to CAC30950.1|AL583924 conserved hypothetical protein from Mycobacterium leprae (78 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P64687 No conserved hypotheticals ML1995 MMAR_0257 Mb0103 NC_000962.3 Mycobrowser_v2 CDS 591475 591576 . + 0 Rv0500B Rv0500B Function unknown Conserved hypothetical protein Rv0500B, len: 33 aa. Conserved hypothetical protein. Basic protein 18 of the 33 aa are Arg or Lys, with strong similarity to AL079345|SCE68_25 protein from Streptomyces coelicolor cosmid E6 (32 aa), FASTA scores: opt: 176, E(): 1e-06, (93.1% identity in 29 aa overlap). Same gene arrangement in both actinomycetes. P0A5C9 No conserved hypotheticals ML2428A NC_000962.3 Mycobrowser_v2 RBS 109992 109996 . + 0 Rv0101 nrp Involved in lipid metabolism. Probable peptide synthetase Nrp (peptide synthase) Rv0101, (MTCY251.20), len: 2512 aa. Probable nrp, peptide synthetase, similar to others e.g. AAD44234.1|AF143772_40|PstB peptide synthetase from Mycobacterium avium (2552 aa); 7476034|S77657 cyclic peptide synthetase from Mycobacterium leprae (1401 aa), FASTA scores: opt: 4268, E(): 0, (65.7% identity in 1091 aa overlap); part of CAB55600.1|AJ238027 peptide synthetase from Mycobacterium smegmatis (5990). Also similar to e.g. AAD56240.1|AF184977_1|AF184977 DhbF protein from Bacillus subtilis (2378 aa); SRF1_BACSU|P27206 surfactin synthetase subunit 1 (3587 aa), FASTA scores: opt: 1708, E(): 0, (30.6% identity in 1633 aa overlap): etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), 2 x PS00455 Putative AMP-binding domain signature, and PS00012 Phosphopantetheine attachment site. Belongs to the ATP-dependent AMP-binding enzyme family. Thought to be not involved in mycobactin biosynthesis (see citation below). Q10896 No lipid metabolism ML1996 Mb0104 NC_000962.3 Mycobrowser_v2 CDS 110001 117539 . + 0 Rv0101 nrp Involved in lipid metabolism. Probable peptide synthetase Nrp (peptide synthase) Rv0101, (MTCY251.20), len: 2512 aa. Probable nrp, peptide synthetase, similar to others e.g. AAD44234.1|AF143772_40|PstB peptide synthetase from Mycobacterium avium (2552 aa); 7476034|S77657 cyclic peptide synthetase from Mycobacterium leprae (1401 aa), FASTA scores: opt: 4268, E(): 0, (65.7% identity in 1091 aa overlap); part of CAB55600.1|AJ238027 peptide synthetase from Mycobacterium smegmatis (5990). Also similar to e.g. AAD56240.1|AF184977_1|AF184977 DhbF protein from Bacillus subtilis (2378 aa); SRF1_BACSU|P27206 surfactin synthetase subunit 1 (3587 aa), FASTA scores: opt: 1708, E(): 0, (30.6% identity in 1633 aa overlap): etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), 2 x PS00455 Putative AMP-binding domain signature, and PS00012 Phosphopantetheine attachment site. Belongs to the ATP-dependent AMP-binding enzyme family. Thought to be not involved in mycobactin biosynthesis (see citation below). Q10896 No lipid metabolism ML1996 Mb0104 NC_000962.3 Mycobrowser_v2 CDS 778990 779487 . + 0 Rv0678 Rv0678 Function unknown Conserved protein Rv0678, (MTV040.06), len: 165 aa. Conserved protein, showing weak similarity with AL049754|SCH10_10 hypothetical protein from Streptomyces coelicolor (152 aa), FASTA scores: opt: 149, E(): 0.0018, (22.9% identity in 140 aa overlap). O53786 No conserved hypotheticals MMAR_1007 Mb0697 NC_000962.3 Mycobrowser_v2 CDS 117714 119699 . + 0 Rv0102 Rv0102 Unknown Probable conserved integral membrane protein Rv0102, (MTCY251.21), len: 661 aa. Probable conserved integral membrane protein, highly similar to P53525|Y102_MYCLE|ML1998|NP_302349.1|NC_002677 possible membrane protein from Mycobacterium leprae (659 aa), FASTA scores: opt: 3107, E(): 0, (70.2% identity in 662 aa overlap). Also similar to others e.g. CAC01497.1|AL391017 putative integral membrane protein from Streptomyces coelicolor (316 aa); etc. Contains PS00343 Gram-positive cocci surface proteins 'anchoring' hexapeptide. P64689 No cell wall and cell processes ML1998 MMAR_0267 MSMEG_4702 Mb0105 NC_000962.3 Mycobrowser_v2 CDS 119915 122173 . - 0 Rv0103c ctpB Cation-transporting ATPase; possibly catalyzes the transport of a cation (possibly coopper) with the hydrolyse of ATP [catalytic activity: ATP + H(2)O + cation(in) = ADP + orthophosphate + cation(out)]. Probable cation-transporter P-type ATPase B CtpB Rv0103c, (MTCY251.22c), len: 752 aa. Probable ctpB, cation-transporting P-type ATPase B (transmembrane protein), equivalent to CTPB_MYCLE|P46840 cation-transporting P-type ATPase B from Mycobacterium leprae (750 aa), FASTA scores: opt: 3615, E(): 0, (76.5% identity in 752 aa overlap). Also highly similar to others e.g. CAB96031.1|AL360055 putative metal transporter ATPase from Streptomyces coelicolor (753 aa); NP_241423.1|NC_002570 copper-transporting ATPase from Bacillus halodurans (806 aa); etc. Also highly similar to Z46257|MLACEA_7 aceA gene for isocitrate L from Mycobacterium leprae (750 aa), FASTA scores: opt: 3615, E():0, (76.5% identity in 752 aa overlap). And similar to MTCY251.11 from Mycobacterium tuberculosis, FASTA score: (68.3% identity in 742 aa overlap). Contains PS01047 Heavy-metal-associated domain, PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases), subfamily IB. Q10877 No cell wall and cell processes ML2000 MMAR_0269 Mb0106c NC_000962.3 Mycobrowser_v2 CDS 122317 123831 . + 0 Rv0104 Rv0104 Function unknown Conserved hypothetical protein Rv0104, (MTCY251.23), len: 504 aa. Conserved hypothetical protein, showing weak similarity with other cAMP-dependent protein kinases e.g. AAC37564.1|M65066 cAMP-dependent protein kinase RI-beta regulatory subunit from Homo sapiens (380 aa); etc. Q10898 No conserved hypotheticals MMAR_0285 Mb0107 NC_000962.3 Mycobrowser_v2 CDS 123980 124264 . - 0 Rv0105c rpmB1 Possibly involved in a translation mechanism. 50S ribosomal protein L28-1 RpmB1 Rv0105c, (MTCY251.24c), len: 94 aa. rpmB1, 50S ribosomal protein L28-1, highly similar to others e.g. Q9X8K8|R28B_STRCO 50S ribosomal protein L28-2 from Streptomyces coelicolor (78 aa); RL28_ECOLI|P02428 50s ribosomal protein l28 from Escherichia coli (77 aa), FASTA scores: opt: 167, E(): 6.2e-06, (40.7% identity in 59 aa overlap); etc. Also similar to MTCY63A_2 from Mycobacterium tuberculosis. Belongs to the L28P family of ribosomal proteins. P0A5V6 No information pathways MMAR_0292 Mb0108c NC_000962.3 Mycobrowser_v2 RBS 124270 124276 . - 0 Rv0105c rpmB1 Possibly involved in a translation mechanism. 50S ribosomal protein L28-1 RpmB1 Rv0105c, (MTCY251.24c), len: 94 aa. rpmB1, 50S ribosomal protein L28-1, highly similar to others e.g. Q9X8K8|R28B_STRCO 50S ribosomal protein L28-2 from Streptomyces coelicolor (78 aa); RL28_ECOLI|P02428 50s ribosomal protein l28 from Escherichia coli (77 aa), FASTA scores: opt: 167, E(): 6.2e-06, (40.7% identity in 59 aa overlap); etc. Also similar to MTCY63A_2 from Mycobacterium tuberculosis. Belongs to the L28P family of ribosomal proteins. P0A5V6 No information pathways MMAR_0292 Mb0108c NC_000962.3 Mycobrowser_v2 CDS 157847 158302 . + 0 Rv0130 htdZ Unknown Probable 3-hydroxyl-thioester dehydratase Rv0130, (MTCI5.04), len: 151 aa. Probable htdZ, 3-hydroxyl-thioester dehydratase. Forms single hot-dog fold, features R-specific hydratase motif, substrate unknown, forms homodimer. Shows structural similarity to six others in Mycobacterium tuberculosis (see Castell et al (2005) below). Similar to others e.g. AL096811|SCI30A_19 from Streptomyces coelicolor (153 aa), FASTA scores: opt: 639, E(): 0, (60.8% identity in 148 aa overlap). P96807 No intermediary metabolism and respiration MMAR_0330 MSMEG_2201 Mb0135 NC_000962.3 Mycobrowser_v2 CDS 2258273 2258671 . + 0 Rv2010 vapC15 Unknown Toxin VapC15 Rv2010, (MTCY39.07c), len: 132 aa. VapC15, toxin, part of toxin-antitoxin (TA) operon with Rv2009, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). P64925 No virulence, detoxification, adaptation Mb2033 NC_000962.3 Mycobrowser_v2 CDS 124374 125570 . + 0 Rv0106 Rv0106 Function unknown Conserved hypothetical protein Rv0106, (MTCY251.25), len: 398 aa. Conserved hypothetical protein, similar to others e.g. AL049841|SCE9_33 from Streptomyces coelicolor (370 aa), FASTA scores: opt: 282, E(): 2.5e-11, (32.0% identity in 381 aa overlap); etc. Some similarity to P94400 homologue to nitrile hydratase region from Bacillus subtilis (397 aa), FASTA scores: opt: 226, E(): 5.4e-08, (26.4% identity in 405 aa overlap). Also similar to COBW_PSEDE|P29937 FASTA score: (25.3% identity in 186 aa overlap); and P47K_PSECL|P31521 47 kDa protein (p47k) (419 aa), FASTA score: (25.9% identity in 401 aa overlap). P64691 No conserved hypotheticals MSMEG_6069 Mb0109 NC_000962.3 Mycobrowser_v2 CDS 677710 677925 . + 0 Rv0581 vapB26 Unknown Possible antitoxin VapB26 Rv0581, (MTV039.19), len: 71 aa. Possible vapB26, antitoxin, part of toxin-antitoxin (TA) operon with Rv0582, see Arcus et al. 2005. Showing weak similarity to other Mycobacterium tuberculosis proteins including P95003|Z83863|Rv2550c|MTCY159_6 conserved hypothetical protein (81 aa), FASTA scores: opt: 93, E(): 3.2, (25.7% identity in 70 aa overlap); Rv2871; Rv1241; etc. Also shows weak similarity to X05648|SGSPH_1 from Streptomyces glaucescens (77 aa), FASTA scores: opt: 92, E(): 3.6, (35.4% identity in 65 aa overlap). O53778 No virulence, detoxification, adaptation Mb0596 NC_000962.3 Mycobrowser_v2 CDS 125643 130541 . - 0 Rv0107c ctpI Cation-transporting ATPase; possibly catalyzes the transport of a cation (possibly magnesium) with the hydrolyse of ATP [catalytic activity: ATP + H(2)O + cation(in) = ADP + phosphate + cation(out)]. Probable cation-transporter ATPase I CtpI Rv0107c, (MTCY251.26c, MTV031.01c), len: 1632 aa. Probable ctpI, cation-transporting ATPase I P-type, highly similar to NP_302704.1|NC_002677 probable cation transport ATPase from Mycobacterium leprae (1609 aa); and similar to others e.g. CAB69720.1|AL137166 putative transport ATPase from Streptomyces coelicolor (1472 aa); ATA1_SYNY|P37367 cation-transporting ATPase pma1 from Synechocystis sp. (915 aa), FASTA scores: opt: 603, E(): 6.6e-29, (32.4% identity in 710 aa overlap); etc. Also similar to MTCY39.21c and MTCY22G10.22c from Mycobacterium tuberculosis, FASTA score: (34.4% identity in 796 aa overlap). Contains PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases). Q10900 No cell wall and cell processes ML2671 Mb0110c,Mb0111c NC_000962.3 Mycobrowser_v2 CDS 130895 131104 . - 0 Rv0108c Rv0108c Unknown Hypothetical protein Rv0108c, (MTV031.02c), len: 69 aa. Hypothetical unknown protein. O53630 No conserved hypotheticals MMAR_0298 Mb0112c NC_000962.3 Mycobrowser_v2 RBS 131372 131375 . + 0 Rv0108c Rv0108c Unknown Hypothetical protein Rv0108c, (MTV031.02c), len: 69 aa. Hypothetical unknown protein. O53630 No conserved hypotheticals MMAR_0298 Mb0112c NC_000962.3 Mycobrowser_v2 CDS 131382 132872 . + 0 Rv0109 PE_PGRS1 Function unknown PE-PGRS family protein PE_PGRS1 Rv0109, (MTV031.03c), len: 496 aa. PE_PGRS1, Member of the M. tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), highly similar to many e.g. Q50615|Y0DP_MYCTU hypothetical glycine-rich 40.8 kDa protein from Mycobacterium tuberculosis (498 aa), FASTA scores: opt: 1772, E(): 0, (57.3% identity in 513 aa overlap); etc. Q79G09 No PE/PPE MMAR_0299 Mb0113 NC_000962.3 Mycobrowser_v2 CDS 133020 133769 . + 0 Rv0110 Rv0110 Unknown Probable conserved integral membrane protein Rv0110, (MTV031.04), len: 249 aa. Probable conserved integral membrane protein, similar to many e.g. AL079308|SCH69_25 from Streptomyces coelicolor (297 aa), FASTA scores: opt: 552, E(): 6.1e-29, (45.4% identity in 251 aa overlap); P54493|YQGP_BACSU hypothetical 56.4 KD protein from Bacillus subtilis (507 aa), FASTA scores: opt: 320, E(): 4e-15, (32.4% identity in 210 aa overlap); etc. O53632 No cell wall and cell processes MSMEG_5036 Mb0114 NC_000962.3 Mycobrowser_v2 CDS 133950 136007 . + 0 Rv0111 Rv0111 Function unknown; probably involved in cellular metabolism. Possible transmembrane acyltransferase Rv0111, (MTV031.05), len: 685 aa. Possible transmembrane acyltransferase, equivalent to AA22904.1|AL035300 putative acyltransferase from Mycobacterium leprae (696 aa). Also similar to others e.g. C69975 acyltransferase homolog yrhL from Bacillus subtilis (634 aa), FASTA scores: opt: 520, E(): 4e-22, (36.4% identity in 382 aa overlap). Very similar to Mycobacterium tuberculosis proteins Rv0228, Rv1254, Rv1565c, etc. O53633 No intermediary metabolism and respiration ML2670 MMAR_0312 MSMEG_5537 Mb0115 NC_000962.3 Mycobrowser_v2 RBS 136278 136281 . + 0 Rv0112 gca Possibly involved in synthesis of a-band common antigen lipopolysaccharide. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose [catalytic activity: GDP-mannose = GDP-4-dehydro-6-deoxy-D-mannose + H2O]. Possible GDP-mannose 4,6-dehydratase Gca (GDP-D-mannose dehydratase) Rv0112, (MTV031.06), len: 318 aa. Possible gca, GDP-mannose 4,6-dehydratase, similar to others e g. U18320|PAU18320_1 GDP-D-mann from Pseudomonas aeruginosa (323 aa), FASTA scores: opt: 415, E(): 4.4e-21, (27.0% identity in 318 aa overlap). Similar to Rv3634c, Rv3784, etc from Mycobacterium tuberculosis. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Seems to belong to the GDP-mannose 4,6-dehydratase family. Cofactor: NAD(+). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53634 No cell wall and cell processes Mb0116 NC_000962.3 Mycobrowser_v2 CDS 136289 137245 . + 0 Rv0112 gca Possibly involved in synthesis of a-band common antigen lipopolysaccharide. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose [catalytic activity: GDP-mannose = GDP-4-dehydro-6-deoxy-D-mannose + H2O]. Possible GDP-mannose 4,6-dehydratase Gca (GDP-D-mannose dehydratase) Rv0112, (MTV031.06), len: 318 aa. Possible gca, GDP-mannose 4,6-dehydratase, similar to others e g. U18320|PAU18320_1 GDP-D-mann from Pseudomonas aeruginosa (323 aa), FASTA scores: opt: 415, E(): 4.4e-21, (27.0% identity in 318 aa overlap). Similar to Rv3634c, Rv3784, etc from Mycobacterium tuberculosis. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Seems to belong to the GDP-mannose 4,6-dehydratase family. Cofactor: NAD(+). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53634 No cell wall and cell processes Mb0116 NC_000962.3 Mycobrowser_v2 CDS 137319 137909 . + 0 Rv0113 gmhA Involved in biosynthesis of nucleotide-activated glycero-manno-heptose: synthesis of glyceromannoheptose 7-phosphate (inner core lipopolysaccharide biosynthesis) [catalytic activity: D-sedoheptulose 7-phosphate = D-glycero-alpha,beta-D-manno-heptose 7-phosphate]. Probable sedoheptulose-7-phosphate isomerase GmhA (phosphoheptose isomerase) Rv0113, (MTV031.07), len: 196 aa. Probable gmhA (alternate gene name: lpcA), sedoheptulose-7-phosphate isomerase (see citation below), similar to many e.g. AE0005|HPAE000596_11 from Helicobacter pylori (192 aa), FASTA scores: opt: 451, E(): 1.9e-24, (45.1% identity in 162 aa overlap). Belongs to the sis family, LPCA subfamily. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P0A604 No cell wall and cell processes Mb0117 NC_000962.3 Mycobrowser_v2 CDS 171215 172168 . + 0 Rv0145 Rv0145 Possible methyltransferase Possible S-adenosylmethionine-dependent methyltransferase Rv0145, (MTCI5.19), len: 317 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), highly similar to many e.g. CAC32172.1|AL583926 conserved hypothetical protein from Mycobacterium leprae (310 aa); and several Mycobacterium tuberculosis proteins e.g. Rv0726c, Rv0731c, etc. P96822 No lipid metabolism ML2641 MMAR_0356 MSMEG_0093 Mb0150 NC_000962.3 Mycobrowser_v2 CDS 158315 159658 . - 0 Rv0131c fadE1 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE1 Rv0131c, (MTCI5.05c), len: 447 aa. Probable fadE1, acyl-CoA dehydrogenase, similar to many e.g. ACDS_HUMAN|P16219 acyl-CoA dehydrogenase short-chain specific precursor (412 aa), FASTA scores: opt: 522, E(): 1.4e-23, (30.1% identity in 425 aa overlap). Also highly similar to MTCI5_28 from Mycobacterium tuberculosis. P96808 No lipid metabolism MMAR_0331 Mb0136c NC_000962.3 Mycobrowser_v2 CDS 137941 138513 . + 0 Rv0114 gmhB Involved in biosynthesis of nucleotide-activated glycero-manno-heptose. Involved in two pathways, D-alpha-D pathway [catalytic activity: D-glycero-alpha-D-manno-heptose 1,7-biphosphate = D-glycero-alpha-D-manno-heptose 1-phosphate] and L-beta-D pathway [catalytic activity: D-glycero-beta-D-manno-heptose 1,7-biphosphate = D-glycero-beta-D-manno-heptose 1-phosphate]. Possible D-alpha,beta-D-heptose-1,7-biphosphate phosphatase GmhB (D-glycero-D-manno-heptose 7-phosphate kinase) Rv0114, (MTV031.08), len: 190 aa. Possible gmhB, D-alpha,beta-D-heptose-1,7-biphosphate phosphatase (see citation below), similar to several hypothetical proteins and phosphatases e.g. HIS7_ECOLI|P06987 imidazoleglycerol-phosphate dehydratase (355 aa), FASTA scores: opt: 250, E(): 3.6e-11, (34.0 % identity in 141 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53636 No cell wall and cell processes Mb0118 NC_000962.3 Mycobrowser_v2 RBS 138498 138503 . + 0 Rv0115 hddA Involved in biosynthesis of nucleotide-activated glycero-manno-heptose (D-alpha-D pathway) [catalytic activity: D-glycero-alpha,beta-D-manno-heptose 7-phosphate + ATP = D-glycero-alpha-D-manno-heptose 1,7-biphosphate]. Possible D-alpha-D-heptose-7-phosphate kinase HddA Rv0115, (MTV031.09), len: 386 aa. Possible hddA, D-alpha-D-heptose-7-phosphate kinase (see citation below), similar to several hypothetical proteins and sugar kinases e.g. AAK27850.1|AF324836_3 D-glycero-D-manno-heptose 7-phosphate kinase from Aneurinibacillus thermoaerophilus (341 aa); AAK80995.1|AE007802_11 Sugar kinase from Clostridium acetobutylicum (364 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53637 No cell wall and cell processes Mb0119 NC_000962.3 Mycobrowser_v2 CDS 138513 139673 . + 0 Rv0115 hddA Involved in biosynthesis of nucleotide-activated glycero-manno-heptose (D-alpha-D pathway) [catalytic activity: D-glycero-alpha,beta-D-manno-heptose 7-phosphate + ATP = D-glycero-alpha-D-manno-heptose 1,7-biphosphate]. Possible D-alpha-D-heptose-7-phosphate kinase HddA Rv0115, (MTV031.09), len: 386 aa. Possible hddA, D-alpha-D-heptose-7-phosphate kinase (see citation below), similar to several hypothetical proteins and sugar kinases e.g. AAK27850.1|AF324836_3 D-glycero-D-manno-heptose 7-phosphate kinase from Aneurinibacillus thermoaerophilus (341 aa); AAK80995.1|AE007802_11 Sugar kinase from Clostridium acetobutylicum (364 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53637 No cell wall and cell processes Mb0119 NC_000962.3 Mycobrowser_v2 CDS 140267 141022 . - 0 Rv0116c ldtA Involved in peptidoglycan synthesis. Catalyzes the formation of 3->3 crosslinks between peptidoglycan subunits. Probable L,D-transpeptidase LdtA Rv0116c, (MTV031.10c), len: 251 aa. Probable ldtA, L,D-transpeptidase, showing similarity to several hypothetical mycobacterial proteins e.g. Rv1433 from Mycobacterium tuberculosis (271 aa); and Q49706|B1496_F2_81|U00013 from Mycobacterium leprae (271 aa); to the C-terminal regions of others like Rv0192 from Mycobacterium tuberculosis (366 aa), FASTA scores: opt: 451, E(): 1.7e-21, (46.7% identity in 270 aa overlap); and Rv0192|Z97050|MTCI28_32 from Mycobacterium tuberculosis cosmid (366 aa), FASTA scores: opt: 699, E(): 0, (45.7% identity in 221 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). O53638 No cell wall and cell processes ML2664 MSMEG_3528 Mb0120c NC_000962.3 Mycobrowser_v2 RBS 141032 141036 . - 0 Rv0116c ldtA Involved in peptidoglycan synthesis. Catalyzes the formation of 3->3 crosslinks between peptidoglycan subunits. Probable L,D-transpeptidase LdtA Rv0116c, (MTV031.10c), len: 251 aa. Probable ldtA, L,D-transpeptidase, showing similarity to several hypothetical mycobacterial proteins e.g. Rv1433 from Mycobacterium tuberculosis (271 aa); and Q49706|B1496_F2_81|U00013 from Mycobacterium leprae (271 aa); to the C-terminal regions of others like Rv0192 from Mycobacterium tuberculosis (366 aa), FASTA scores: opt: 451, E(): 1.7e-21, (46.7% identity in 270 aa overlap); and Rv0192|Z97050|MTCI28_32 from Mycobacterium tuberculosis cosmid (366 aa), FASTA scores: opt: 699, E(): 0, (45.7% identity in 221 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). O53638 No cell wall and cell processes ML2664 MSMEG_3528 Mb0120c NC_000962.3 Mycobrowser_v2 RBS 141189 141194 . + 0 Rv0117 oxyS Could effect functions of OXYR during evolution. Oxidative stress response regulatory protein OxyS Rv0117, (MTV031.11), len: 314 aa. OxyS, oxidative stress response protein regulatory protein, LysR family (see citation below). Similar to many transcription regulators and OxyR, the oxidative stress response protein of many bacteria. Contains LysR family signature at N-terminus. Also contains helix-turn-helix motif at aa 16-37 (Score 1543, +4.44 SD). Belongs to the LysR family of transcriptional regulators. OXYR is required for the induction of a regulon of hydrogen peroxide inducible genes such as catalase, glutathione-reductase, etc. O33175 No regulatory proteins ML2663 MMAR_0317 MSMEG_0156 Mb0121 NC_000962.3 Mycobrowser_v2 CDS 141200 142144 . + 0 Rv0117 oxyS Could effect functions of OXYR during evolution. Oxidative stress response regulatory protein OxyS Rv0117, (MTV031.11), len: 314 aa. OxyS, oxidative stress response protein regulatory protein, LysR family (see citation below). Similar to many transcription regulators and OxyR, the oxidative stress response protein of many bacteria. Contains LysR family signature at N-terminus. Also contains helix-turn-helix motif at aa 16-37 (Score 1543, +4.44 SD). Belongs to the LysR family of transcriptional regulators. OXYR is required for the induction of a regulon of hydrogen peroxide inducible genes such as catalase, glutathione-reductase, etc. O33175 No regulatory proteins ML2663 MMAR_0317 MSMEG_0156 Mb0121 NC_000962.3 Mycobrowser_v2 CDS 2123174 2123611 . + 0 Rv1873 Rv1873 Unknown Conserved hypothetical protein Rv1873, (MTCY180.45c), len: 145 aa. Conserved hypothetical protein. Some similarity to AL591783 hypothetical protein from Sinorhizobium meliloti. O07756 No conserved hypotheticals MSMEG_3577 Mb1904 NC_000962.3 Mycobrowser_v2 CDS 142128 143876 . - 0 Rv0118c oxcA Involved in catabolism of oxalic acid [catalytic activity: oxalyl-CoA = formyl-CoA + CO2]. Probable oxalyl-CoA decarboxylase OxcA Rv0118c, (MTV031.12c), Len: 582 aa. Probable oxcA, oxalyl-CoA decarboxylase, highly similar to many e.g. P78093|OXC_ECOLI|7449483|B65011|YFDU|B2373|Z3637|ECS325 probable oxalyl-CoA decarboxylase from Escherichia coli (564 aa); M77128|OXAOXA_1 oxalyl-CoA decarboxylase from Oxalobacter formigenes (568 aa), FASTA scores: opt: 2124, E():0, (55.6% identity in 568 aa overlap). Also similar to mycobacterial IlvB proteins e.g. MLCB1788.46c unknown TPP-requiring enzyme from Mycobacterium leprae (548 aa); and AL0086|MLCB1788_19 from Mycobacterium leprae (548 aa), FASTA scores: opt: 831, E(): 0, (33.9% identity in 567 aa overlap). O53639 No intermediary metabolism and respiration ML2662 MMAR_0318 MSMEG_0157,MSMEG_5297 Mb0122c NC_000962.3 Mycobrowser_v2 RBS 144035 144040 . + 0 Rv0119 fadD7 Function unknown, but involved in lipid degradation. Probable fatty-acid-CoA ligase FadD7 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv0119, (MTV031.13-MTCI418B.01), len: 525 aa. Probable fadD7, fatty-acid-CoA synthetase, similar to 4-coumarate:CoA ligase of many organisms e.g. U39405|PTU39405_1 4-coumarate:CoA ligase from Pinus taedaxylem (537 aa), FASTA scores: opt: 483, E(): 8.3e-22, (28.2% identity in 440 aa overlap). Contains PS00455 Putative AMP-binding domain signature. O07169 No lipid metabolism ML2661 MSMEG_5295 Mb0123 NC_000962.3 Mycobrowser_v2 CDS 144049 145626 . + 0 Rv0119 fadD7 Function unknown, but involved in lipid degradation. Probable fatty-acid-CoA ligase FadD7 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv0119, (MTV031.13-MTCI418B.01), len: 525 aa. Probable fadD7, fatty-acid-CoA synthetase, similar to 4-coumarate:CoA ligase of many organisms e.g. U39405|PTU39405_1 4-coumarate:CoA ligase from Pinus taedaxylem (537 aa), FASTA scores: opt: 483, E(): 8.3e-22, (28.2% identity in 440 aa overlap). Contains PS00455 Putative AMP-binding domain signature. O07169 No lipid metabolism ML2661 MSMEG_5295 Mb0123 NC_000962.3 Mycobrowser_v2 CDS 677922 678329 . + 0 Rv0582 vapC26 Unknown Possible toxin VapC26. Contains PIN domain. Rv0582, (MTV039.20), len: 135 aa. Possible vapC26, toxin, part of toxin-antitoxin (TA) operon with Rv0581, contains PIN domain, see Arcus et al. 2005. O53779 No virulence, detoxification, adaptation Mb0597 NC_000962.3 Mycobrowser_v2 CDS 145627 147771 . - 0 Rv0120c fusA2 Involved in translation mechanism. This protein may promote the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. Probable elongation factor G FusA2 (EF-G) Rv0120c, (MTCI418B.02c), len: 714 aa. Probable fusA2 (alternate gene name: fus2), elongation factor G, highly similar to others e.g. EFG_ECOLI|P02996 elongation factor G (ef-g) from Escherichia coli (703 aa), FASTA scores: opt: 1049, E(): 0, (32.5% identity in 717 aa overlap). Also similar to fusA1|MTCY210.01 from Mycobacterium tuberculosis FASTA score: (39.1% identity in 299 aa overlap); and P30767|EFG_MYCLE elongation factor G (EF-G) from Mycobacterium leprae (701 aa), FASTA score: (31.7% identity in 710 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the GTP-binding elongation factor family, EF-G/EF-2 subfamily. O07170 No information pathways ML2660 MMAR_0321 MSMEG_6535 Mb0124c,Mb0125c NC_000962.3 Mycobrowser_v2 CDS 147908 148342 . - 0 Rv0121c Rv0121c Function unknown Conserved protein Rv0121c, (MTCI418B.03c), len: 144 aa. Conserved protein, showing some similarity with others proteins from Mycobacterium tuberculosis e.g. Rv1155, Rv1875, Rv2074, etc. O07171 No conserved hypotheticals MMAR_0322 MSMEG_6526 Mb0126c NC_000962.3 Mycobrowser_v2 RBS 148351 148353 . - 0 Rv0121c Rv0121c Function unknown Conserved protein Rv0121c, (MTCI418B.03c), len: 144 aa. Conserved protein, showing some similarity with others proteins from Mycobacterium tuberculosis e.g. Rv1155, Rv1875, Rv2074, etc. O07171 No conserved hypotheticals MMAR_0322 MSMEG_6526 Mb0126c NC_000962.3 Mycobrowser_v2 RBS 148484 148487 . + 0 Rv0122 Rv0122 Unknown Hypothetical protein Rv0122, (MTCI418B.04), len: 122 aa. Hypothetical unknown protein. O07172 No conserved hypotheticals Mb0127 NC_000962.3 Mycobrowser_v2 CDS 148491 148859 . + 0 Rv0122 Rv0122 Unknown Hypothetical protein Rv0122, (MTCI418B.04), len: 122 aa. Hypothetical unknown protein. O07172 No conserved hypotheticals Mb0127 NC_000962.3 Mycobrowser_v2 RBS 148842 148847 . + 0 Rv0123 Rv0123 Unknown Unknown protein Rv0123, (MTCI418B.05), len: 122 aa. Unknown protein. O07173 No conserved hypotheticals Mb0128 NC_000962.3 Mycobrowser_v2 CDS 148856 149224 . + 0 Rv0123 Rv0123 Unknown Unknown protein Rv0123, (MTCI418B.05), len: 122 aa. Unknown protein. O07173 No conserved hypotheticals Mb0128 NC_000962.3 Mycobrowser_v2 CDS 289345 289782 . + 0 Rv0240 vapC24 Unknown Possible toxin VapC24. Contains PIN domain. Rv0240, (MTV034.06), len: 145 aa. Possible vapC24, toxin, part of toxin-antitoxin (TA) operon with Rv0239, contains PIN domain, weak similarity with Rv3697c from Mycobacterium tuberculosis (145 aa). See Arcus et al. 2005. FASTA scores: opt: 145, E(): 7.6e-05, (28.0% identity in 143 aa overlap). O53663 No virulence, detoxification, adaptation Mb0246 NC_000962.3 Mycobrowser_v2 RBS 149522 149526 . + 0 Rv0124 PE_PGRS2 Function unknown PE-PGRS family protein PE_PGRS2 Rv0124, (MTCI418B.06), len: 487 aa. PE_PGRS2, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), highly similar to many e.g. Y0DP_MYCTU|Q50615 from Mycobacterium tuberculosis (498 aa), FASTA scores: opt: 1730, E(): 0, (60.7% identity in 504 aa overlap). Q79G08 No PE/PPE MMAR_0572,MMAR_2053,MMAR_2097,MMAR_2248,MMAR_2492,MMAR_2596,MMAR_2709,MMAR_2970,MMAR_3199,MMAR_4127,MMAR_4149,MMAR_4548,MMAR_4561,MMAR_4562 MSMEG_3202 Mb0129 NC_000962.3 Mycobrowser_v2 CDS 149533 150996 . + 0 Rv0124 PE_PGRS2 Function unknown PE-PGRS family protein PE_PGRS2 Rv0124, (MTCI418B.06), len: 487 aa. PE_PGRS2, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), highly similar to many e.g. Y0DP_MYCTU|Q50615 from Mycobacterium tuberculosis (498 aa), FASTA scores: opt: 1730, E(): 0, (60.7% identity in 504 aa overlap). Q79G08 No PE/PPE MMAR_0572,MMAR_2053,MMAR_2097,MMAR_2248,MMAR_2492,MMAR_2596,MMAR_2709,MMAR_2970,MMAR_3199,MMAR_4127,MMAR_4149,MMAR_4548,MMAR_4561,MMAR_4562 MSMEG_3202 Mb0129 NC_000962.3 Mycobrowser_v2 CDS 167271 167681 . - 0 Rv0141c Rv0141c Unknown Unknown protein Rv0141c, (MTCI5.15c), len: 136 aa. Unknown protein. P96818 No conserved hypotheticals ML2644 MMAR_0351 Mb0146c NC_000962.3 Mycobrowser_v2 CDS 167711 168637 . + 0 Rv0142 Rv0142 Function unknown Conserved hypothetical protein Rv0142, (MTCI5.16), len: 308 aa. Conserved hypothetical protein, similar, except in N-terminus, to AB88922.1|AL353862 hypothetical protein SCE34.20 from Streptomyces coelicolor (326 aa). P96819 No conserved hypotheticals MMAR_0352 MSMEG_6468 Mb0147 NC_000962.3 Mycobrowser_v2 RBS 151135 151140 . + 0 Rv0125 pepA Function unknown; possibly hydrolyzes peptides and/or proteins (seems to cleave preferentially after serine residues). Probable serine protease PepA (serine proteinase) (MTB32A) Rv0125, (MTCI418B.07, MTB32A), len: 355 aa. Probable pepA (alternate gene name: mtb32a), serine protease (see Skeiky et al., 1999), highly similar to other proteases e.g. HHOB_ECOLI|P31137 protease hhob precursor (355 aa), FASTA scores: opt: 400, E(): 3.8e-14, (32.4% identity in 346 aa overlap). Also similar to Q50320 34 kDa protein precursor from Mycobacterium tuberculosis (361 aa), FASTA scores: opt: 1689, E(): 0, (70.7% identity in 362 aa overlap). Contains PS00135 Serine proteases, trypsin family, serine active site. Has a putative signal sequence at the N-terminus. Belongs to the serine protease family. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). O07175 No intermediary metabolism and respiration ML2659 MMAR_0324 Mb0130 NC_000962.3 Mycobrowser_v2 CDS 151148 152215 . + 0 Rv0125 pepA Function unknown; possibly hydrolyzes peptides and/or proteins (seems to cleave preferentially after serine residues). Probable serine protease PepA (serine proteinase) (MTB32A) Rv0125, (MTCI418B.07, MTB32A), len: 355 aa. Probable pepA (alternate gene name: mtb32a), serine protease (see Skeiky et al., 1999), highly similar to other proteases e.g. HHOB_ECOLI|P31137 protease hhob precursor (355 aa), FASTA scores: opt: 400, E(): 3.8e-14, (32.4% identity in 346 aa overlap). Also similar to Q50320 34 kDa protein precursor from Mycobacterium tuberculosis (361 aa), FASTA scores: opt: 1689, E(): 0, (70.7% identity in 362 aa overlap). Contains PS00135 Serine proteases, trypsin family, serine active site. Has a putative signal sequence at the N-terminus. Belongs to the serine protease family. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). O07175 No intermediary metabolism and respiration ML2659 MMAR_0324 Mb0130 NC_000962.3 Mycobrowser_v2 RBS 152317 152319 . + 0 Rv0126 treS Involved in trehalose biosynthesis (protective effect). Converts maltose to trehalose. Mycobacteria can produce trehalose from glucose 6-phosphate and UDP-glucose (the OtsA-OtsB pathway) from glycogen-like alpha(1-->4)-linked glucose polymers (the TreY-TreZ pathway) and from maltose (the TreS pathway). Trehalose synthase TreS Rv0126, (MTCI418B.08), len: 601 aa. TreS, trehalose synthase (see citation below), highly similar to others e.g. CAA04601.2|AJ001205 putative trehalose synthase from Streptomyces coelicolor (566 aa); S71450|1536814|BAA11303.1|D78198 trehalose synthase maltose-specific from Pimelobacter sp. strain R48 (573 aa). Also similar to MAL1_DROME|P07191 possible maltase precursor (508 aa), FASTA scores: opt: 807, E(): 0, (33.7% identity in 504 aa overlap); and similar to proteins associated with amino-acid transport e.g. Q64319 rat protein which stimulates transport of cystine and dibasic and neutral amino acids (683 aa), FASTA scores: opt: 839, E(): 0, (32.0% identity in 531 aa overlap). Also similar to several other Mycobacterium tuberculosis proteins e.g. Rv2471 FASTA score: (31.7% identity in 164 aa overlap). O07176 No virulence, detoxification, adaptation ML2658 MMAR_0325 MSMEG_6515 Mb0131 NC_000962.3 Mycobrowser_v2 CDS 152324 154129 . + 0 Rv0126 treS Involved in trehalose biosynthesis (protective effect). Converts maltose to trehalose. Mycobacteria can produce trehalose from glucose 6-phosphate and UDP-glucose (the OtsA-OtsB pathway) from glycogen-like alpha(1-->4)-linked glucose polymers (the TreY-TreZ pathway) and from maltose (the TreS pathway). Trehalose synthase TreS Rv0126, (MTCI418B.08), len: 601 aa. TreS, trehalose synthase (see citation below), highly similar to others e.g. CAA04601.2|AJ001205 putative trehalose synthase from Streptomyces coelicolor (566 aa); S71450|1536814|BAA11303.1|D78198 trehalose synthase maltose-specific from Pimelobacter sp. strain R48 (573 aa). Also similar to MAL1_DROME|P07191 possible maltase precursor (508 aa), FASTA scores: opt: 807, E(): 0, (33.7% identity in 504 aa overlap); and similar to proteins associated with amino-acid transport e.g. Q64319 rat protein which stimulates transport of cystine and dibasic and neutral amino acids (683 aa), FASTA scores: opt: 839, E(): 0, (32.0% identity in 531 aa overlap). Also similar to several other Mycobacterium tuberculosis proteins e.g. Rv2471 FASTA score: (31.7% identity in 164 aa overlap). O07176 No virulence, detoxification, adaptation ML2658 MMAR_0325 MSMEG_6515 Mb0131 NC_000962.3 Mycobrowser_v2 CDS 208417 208971 . + 0 Rv0177 Rv0177 Unknown Probable conserved Mce associated protein Rv0177, (MTCI28.17), len: 184 aa. Probable conserved Mce-associated protein, equivalent to CAC32129.1|AL583926 conserved membrane protein from Mycobacterium leprae (184 aa). Also similar to mce-associated proteins from Mycobacterium tuberculosis e.g. Rv1363c, Rv1973, Rv3493c, etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O07421 No conserved hypotheticals ML2597 MMAR_0420 MSMEG_0142 Mb0183 NC_000962.3 Mycobrowser_v2 RBS 2263417 2263421 . + 0 Rv2016 Rv2016 Unknown Hypothetical protein Rv2016, (MTV018.03), len: 191 aa. Hypothetical protein. O53462 No conserved hypotheticals Mb2039 NC_000962.3 Mycobrowser_v2 CDS 2263426 2264001 . + 0 Rv2016 Rv2016 Unknown Hypothetical protein Rv2016, (MTV018.03), len: 191 aa. Hypothetical protein. O53462 No conserved hypotheticals Mb2039 NC_000962.3 Mycobrowser_v2 RBS 154216 154221 . + 0 Rv0127 mak Catalyzes the transfer of a phosphate group from ATP to maltose to produce maltose-1-phosphate [catalytic activity: ATP + maltose = ADP + maltose-1-phosphate] Maltokinase Mak Rv0127, (MTCI418B.09, MTCI5.01), len: 455 aa. Mak, maltokinase; highly similar to various proteins e.g. AJ0012|SCJ001205_4 hypothetical protein from Streptomyces coelicolor A3(2) (464 aa), FASTA scores: opt: 412, E(): 1.1e-19, (40.6% identity in 485 aa overlap); AJ0012|SCJ001206_5 hypothetical protein from Streptomyces coelicolor A3(2) (453 aa), FASTA scores: opt: 403, E(): 4.3 e-19, (36.5% identity in 455 aa overlap). O07177 No virulence, detoxification, adaptation ML2657 MMAR_0326 MSMEG_6514 Mb0132 NC_000962.3 Mycobrowser_v2 CDS 154232 155599 . + 0 Rv0127 mak Catalyzes the transfer of a phosphate group from ATP to maltose to produce maltose-1-phosphate [catalytic activity: ATP + maltose = ADP + maltose-1-phosphate] Maltokinase Mak Rv0127, (MTCI418B.09, MTCI5.01), len: 455 aa. Mak, maltokinase; highly similar to various proteins e.g. AJ0012|SCJ001205_4 hypothetical protein from Streptomyces coelicolor A3(2) (464 aa), FASTA scores: opt: 412, E(): 1.1e-19, (40.6% identity in 485 aa overlap); AJ0012|SCJ001206_5 hypothetical protein from Streptomyces coelicolor A3(2) (453 aa), FASTA scores: opt: 403, E(): 4.3 e-19, (36.5% identity in 455 aa overlap). O07177 No virulence, detoxification, adaptation ML2657 MMAR_0326 MSMEG_6514 Mb0132 NC_000962.3 Mycobrowser_v2 CDS 155667 156446 . + 0 Rv0128 Rv0128 Unknown Probable conserved transmembrane protein Rv0128, (MTCI5.02), len: 259 aa. Probable conserved transmembrane protein, with some similarity to Rv3064c and other bacterial proteins e.g. AAK85977.1|AE007957|AGR_C_254p from Agrobacterium tumefaciens (206 aa). P96805 No cell wall and cell processes ML2656 MMAR_0327 Mb0133 NC_000962.3 Mycobrowser_v2 CDS 475816 476184 . + 0 Rv0397 Rv0397 Function unknown Conserved 13E12 repeat family protein Rv0397, (MTCY04D9.09), len: 122 aa. Part of 13E12 repeat family of conserved Mycobacterium tuberculosis proteins, similar to downstream Rv0393|Z84725|MTCY4D9_5 conserved 13E12 repeat family protein (441 aa), FASTA scores: E(): 0, (87.7% identity in 122 aa overlap). P95205 No insertion seqs and phages Mb0403 NC_000962.3 Mycobrowser_v2 CDS 156578 157600 . - 0 Rv0129c fbpC Proteins of the antigen 85 complex are responsible for the high affinity of mycobacteria to fibronectin. Possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity. Secreted antigen 85-C FbpC (85C) (antigen 85 complex C) (AG58C) (mycolyl transferase 85C) (fibronectin-binding protein C) Rv0129c, (MT0137, MTCI5.03c), len: 340 aa. FbpC (alternate gene names: mpt45, 85C, fbpC2), secreted antigen 85c (fibronectin-binding protein C) (mycolyl transferase 85C) (see citations below), also highly similar to other Mycobacterial antigen precursors e.g. A85C_MYCLE|Q05862 antigen 85-c precursor (85c) from Mycobacterium leprae (333 aa), FASTA scores: opt: 1937, E(): 0, (81.4% identity in 333 aa overlap); etc. P0A4V4 No lipid metabolism ML2655 MMAR_0328 MSMEG_3580 Mb0134c NC_000962.3 Mycobrowser_v2 CDS 237892 238395 . - 0 Rv0201c Rv0201c Function unknown Conserved protein Rv0201c, (MTV033.09c), len: 167 aa. Conserved protein, equivalent to Z95398|MLCL622.15c from Mycobacterium leprae (170 aa), FASTA scores: opt: 646, E(): 0, (63.9% identity in 158 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53652 No conserved hypotheticals ML2616 MMAR_0441 MSMEG_0240 Mb0207c NC_000962.3 Mycobrowser_v2 RBS 238400 238405 . - 0 Rv0201c Rv0201c Function unknown Conserved protein Rv0201c, (MTV033.09c), len: 167 aa. Conserved protein, equivalent to Z95398|MLCL622.15c from Mycobacterium leprae (170 aa), FASTA scores: opt: 646, E(): 0, (63.9% identity in 158 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53652 No conserved hypotheticals ML2616 MMAR_0441 MSMEG_0240 Mb0207c NC_000962.3 Mycobrowser_v2 CDS 164712 165260 . - 0 Rv0137c msrA Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine [catalytic activity: protein L-methionine + oxidized thioredoxin + H2O = protein-L-methionine-(S)-S-oxide + reduced thioredoxin]. Probable peptide methionine sulfoxide reductase MsrA (protein-methionine-S-oxide reductase) (peptide met(O) reductase) Rv0137c, (MTCI5.11c), len: 182 aa. Probable msrA, peptide methionine sulfoxide reductase (See St. John et al., 2001), equivalent to CAC32179.1|AL583926 putative peptide methionine sulfoxide from Mycobacterium leprae (177 aa). Highly similar to others e.g. CAC18703.1|AL451182 putative peptide methionine sulfoxide reductase from Streptomyces coelicolor (172 aa); PMSR_SCHPO|Q09859 putative peptide methionine sulfoxide reductase from Streptomyces (187 aa), FASTA scores: opt: 468, E(): 9.9e-26, (45.6% identity in 158 aa overlap); etc. Belongs to the MsrA family. P0A5L0 No intermediary metabolism and respiration ML2647 MMAR_0347 MSMEG_6477 Mb0142c NC_000962.3 Mycobrowser_v2 CDS 159700 160782 . - 0 Rv0132c fgd2 Catalyzes oxidation of glucose-6-phosphate to 6-phosphogluconolactone using coenzyme F420 (an *-hydroxy-5-deazaflavin derivative) as the electron acceptor. Putative F420-dependent glucose-6-phosphate dehydrogenase Fgd2 Rv0132c, (MTCI5.06c), len: 360 aa. Putative fgd2, F420-dependent glucose-6-phosphate dehydrogenase, highly similar to many from Mycobacteria e.g. AAD38167|g5031431 from Mycobacterium chelonae. Also similar to MJ1534|Q58929 N5,N10-methylene tetrahydromethanopterin reductase from methanococcus jannaschii (342 aa), FASTA scores: opt: 285, E(): 7.9e-11, (28.4% identity in 292 aa overlap). And also similar to Rv0953c, Rv0791c, etc from Mycobacterium tuberculosis. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. P96809 No intermediary metabolism and respiration Mb0137c NC_000962.3 Mycobrowser_v2 CDS 160869 161474 . + 0 Rv0133 Rv0133 Acetylation, substrate unknown GCN5-related N-acetyltransferase Rv0133, (MTCI5.07), len: 201 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain in C-terminal part. See Vetting et al. 2005. Highly similar to others e.g. PUAC_STRLP|P13249 puromycyn N-acetyltransferase (199 aa), FASTA scores: opt: 341, E(): 1.8e-16, (33.3% identity in 201 aa overlap). P96810 No intermediary metabolism and respiration MMAR_0332 MSMEG_4563 Mb0138 NC_000962.3 Mycobrowser_v2 CDS 161771 162673 . + 0 Rv0134 ephF Thought to be involved in detoxification reactions following oxidative damage to lipids [catalytic activity: an epoxide + H(2)O = a glycol]. Possible epoxide hydrolase EphF (epoxide hydratase) (arene-oxide hydratase) Rv0134, (MTCI5.08), len: 300 aa. Possible ephE, epoxide hydrolase (see citation below), similar to others e.g. Q39856 epoxide hydrolase (341 aa), FASTA scores: opt: 369, E(): 4.6e-17, (27.2% identity in 335 aa overlap); etc. Also similar to MTCY09F9.26c from Mycobacterium tuberculosis (29.5% identity in 346 aa overlap). P96811 No virulence, detoxification, adaptation MMAR_0342 Mb0139 NC_000962.3 Mycobrowser_v2 CDS 257783 258856 . - 0 Rv0215c fadE3 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE3 Rv0215c, (MTCY08D5.10c), len: 357 aa. Probable fadE3, acyl- dehydrogenase, similar to many e.g. ACDB_BACSU|P45857 acyl-CoA dehydrogenase from B. subtilis (379 aa), FASTA scores: opt: 812, E(): 0, (39.5% identity in 354 aa overlap). P96397 No lipid metabolism ML2626 MMAR_0455,MMAR_4161 MSMEG_1991 Mb0221c NC_000962.3 Mycobrowser_v2 CDS 163366 164691 . + 0 Rv0136 cyp138 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Probable cytochrome P450 138 Cyp138 Rv0136, (MT0144, MTCI5.10), len: 441 aa. Probable cyp138, cytochrome P450 138, similar to others e.g. SLR0574|Q59990 from synechocystis SP. (444 aa), FASTA scores: opt: 315, E(): 1e-13, (25.7% identity in 416 aa overlap); etc. Also similar to MTV039_6 from Mycobacterium tuberculosis (472 aa), FASTA score: (38.2% identity in 442 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop); and PS00086 Cytochrome P450 cysteine heme-iron ligand signature. Belongs to the cytochrome P450 family. P63717 No intermediary metabolism and respiration ML2648 MMAR_0346 MSMEG_6478 Mb0141 NC_000962.3 Mycobrowser_v2 ncRNA 293604 293705 . + 0 MTB000051 F6 Unknown Putative small regulatory RNA F6, putative small regulatory RNA (See Arnvig and Young, 2009; DiChiara et al., 2010). Alternate 3'-ends at positions 293641 and 293661. No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 165827 166849 . + 0 Rv0139 Rv0139 Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0139, (MTCI5.13), len: 340 aa. Possible oxidoreductase, similar to others e.g. O34285|HPNA HPNA protein from Zymomonas mobilis (337 aa), FASTA scores: opt: 507, E (): 5.8e-27, (31.1% identity in 328 aa overlap); TRE_STRGR|P29782 dtdp-glucose 4,6-dehydratase (328 aa), FASTA scores: opt: 254, E(): 2.6e-10, (29.0% identity in 307 aa overlap). P96816 No intermediary metabolism and respiration ML2645 MMAR_0349 MSMEG_6474 Mb0144 NC_000962.3 Mycobrowser_v2 CDS 166910 167290 . + 0 Rv0140 Rv0140 Function unknown Conserved protein Rv0140, (MTCI5.14), len: 126 aa. Conserved protein, similar to others e.g. P74567|D90916_48 hypothetical 20.8 KDP protein from Synechocystis sp. (180 aa), FASTA scores: opt: 229, E(): 4.7e-10, (36.1% identity in 108 aa overlap). Also similar to Rv1056 and Rv1670 from Mycobacterium tuberculosis. P96817 No conserved hypotheticals MMAR_0350 MSMEG_6469 Mb0145 NC_000962.3 Mycobrowser_v2 RBS 223592 223596 . + 0 Rv0192A Rv0192A Function unknown Conserved secreted protein Rv0192A, len: 100 aa. Probable N-terminal part of Rv0192, which is member of family P5.17 with Rv0116c, Rv1433, Rv2518c, Rv0483. These are all predicted to be exported/membrane proteins. Rv0192A has typical N-terminal signal peptide which is functional and was identified by PhoA fusion screens: O52054 PGB14T-O1 precursor (fragment 45 AA) (see Chubb et al., 1998). Since Rv0192 misses a signal peptide this suggests that there is a frameshift in the region of the overlap with Rv0192 but none found on reinspection of sequence. Q79FZ8 No cell wall and cell processes Mb0198 NC_000962.3 Mycobrowser_v2 CDS 223607 223909 . + 0 Rv0192A Rv0192A Function unknown Conserved secreted protein Rv0192A, len: 100 aa. Probable N-terminal part of Rv0192, which is member of family P5.17 with Rv0116c, Rv1433, Rv2518c, Rv0483. These are all predicted to be exported/membrane proteins. Rv0192A has typical N-terminal signal peptide which is functional and was identified by PhoA fusion screens: O52054 PGB14T-O1 precursor (fragment 45 AA) (see Chubb et al., 1998). Since Rv0192 misses a signal peptide this suggests that there is a frameshift in the region of the overlap with Rv0192 but none found on reinspection of sequence. Q79FZ8 No cell wall and cell processes Mb0198 NC_000962.3 Mycobrowser_v2 CDS 168704 170182 . - 0 Rv0143c Rv0143c Unknown; possibly ion channel involved in transport of chloride across the membrane. Probable conserved transmembrane protein Rv0143c, (MTCI5.17c), len: 492 aa. Probable conserved transmembrane protein, CIC family possibly involved in transport of chloride, similar to others and hypothetical proteins e.g. O28857 putative chloride channel from Archaeoglobus fulgidus (589 aa), FASTA scores: opt: 966, E(): 0, (37.7% identity in 453 aa overlap); YADQ_ECOLI|P37019 hypothetical 46.0 kDa protein (436 aa), FASTA scores: opt: 452, E(): 2.4e-20, (28.0% identity in 460 aa overlap). P96820 No cell wall and cell processes ML2643 MMAR_0353 MSMEG_6465 Mb0148c NC_000962.3 Mycobrowser_v2 CDS 170284 171126 . + 0 Rv0144 Rv0144 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (possibly TetR-family) Rv0144, (MTCI5.18), len: 280 aa. Probable transcriptional regulator, possibly TetR family. Has region similar to others e.g. Q59431|UIDR_ECOLI|GUSR|B1618|Z2623|ECS2326 UID operon repressor (GUS operon) from Escherichia coli strains K12 and O157:H7 (196 aa), FASTA scores: opt: 214, E(): 1.1e-06, (26.0% identity in 196 aa overlap). Contains probable helix-turn helix motif from aa 109-130 (Score 1463, +4.17 SD). Could belong to the TetR/AcrR family of transcriptional regulators. P96821 No regulatory proteins ML2642 MMAR_0355 MSMEG_0092 Mb0149 NC_000962.3 Mycobrowser_v2 CDS 172211 173143 . + 0 Rv0146 Rv0146 Possible methyltransferase Possible S-adenosylmethionine-dependent methyltransferase Rv0146, (MTCI5.20), len: 310 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), highly similar to others e.g. AC30975.1|AL583924 conserved hypothetical protein from Mycobacterium leprae (304 aa); and several Mycobacterium tuberculosis proteins e.g. Rv0726c, Rv0731c, etc. P96823 No lipid metabolism ML2640 Mb0151 NC_000962.3 Mycobrowser_v2 CDS 173238 174758 . + 0 Rv0147 Rv0147 Function unknown; probably involved in cellular metabolism [catalytic activity: an aldehyde + NAD+ + H2O = an acid + NADH]. Probable aldehyde dehydrogenase (NAD+) dependent Rv0147, (MTCI5.21), len: 506 aa. Probable aldehyde dehydrogenase (NAD+) dependent, similar to others e.g. DHAP_RAT|P11883 aldehyde dehydrogenase (dimeric NADP-preferring) (452 aa), FASTA scores: opt: 1291, E(): 0, (43.9% identity in 453 aa overlap). Also similar to several Mycobacterium tuberculosis aldehyde dehydrogenases e.g. Rv0768, Rv2858c, etc. Contains PS00687 aldehyde dehydrogenases glutamic acid active site, and PS00070 aldehyde dehydrogenases cysteine active site. Belongs to the aldehyde dehydrogenases family. P96824 No intermediary metabolism and respiration ML2639 MMAR_0359 Mb0152 NC_000962.3 Mycobrowser_v2 promoter 174782 174803 . + 0 Rv0148 Rv0148 Function unknown; possibly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv0148, (MTCI5.22), len: 286 aa. Probable short-chain dehydrogenase, similar to others, in particular Estradiol 17 beta-dehydrogenases, e.g. DHB4_MOUSE|P51660 estradiol 17 beta-dehydrogenase 4 (735 aa), FASTA scores: opt: 952, E(): 0, (52.5% identity in 276 aa overlap). Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. P96825 No intermediary metabolism and respiration Mb0153 NC_000962.3 Mycobrowser_v2 RBS 174824 174828 . + 0 Rv0148 Rv0148 Function unknown; possibly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv0148, (MTCI5.22), len: 286 aa. Probable short-chain dehydrogenase, similar to others, in particular Estradiol 17 beta-dehydrogenases, e.g. DHB4_MOUSE|P51660 estradiol 17 beta-dehydrogenase 4 (735 aa), FASTA scores: opt: 952, E(): 0, (52.5% identity in 276 aa overlap). Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. P96825 No intermediary metabolism and respiration Mb0153 NC_000962.3 Mycobrowser_v2 CDS 174833 175693 . + 0 Rv0148 Rv0148 Function unknown; possibly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv0148, (MTCI5.22), len: 286 aa. Probable short-chain dehydrogenase, similar to others, in particular Estradiol 17 beta-dehydrogenases, e.g. DHB4_MOUSE|P51660 estradiol 17 beta-dehydrogenase 4 (735 aa), FASTA scores: opt: 952, E(): 0, (52.5% identity in 276 aa overlap). Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. P96825 No intermediary metabolism and respiration Mb0153 NC_000962.3 Mycobrowser_v2 CDS 175700 176668 . + 0 Rv0149 Rv0149 Possibly binds NADP and acts through a one-electron transfer process. Quinones are supposed to be the best substrates. May act in the detoxification of xenobiotics [catalytic activity: NADPH + quinone = NADP+ + semiquinone] Possible quinone oxidoreductase (NADPH:quinone oxidoreductase) (zeta-crystallin) Rv0149, (MTCI5.23), len: 322 aa. Possible quinone oxidoreductase, similar to others oxidoreductases e.g. Q08257 quinone oxidoreductase (329 aa), FASTA scores: opt: 397, E(): 3.2e-18, (28.4% identity in 328 aa overlap); SCHCOADH_4 from Streptomyces coelicolor. Also similar to many proteins from Mycobacterium tuberculosis. Contains PS01162 Quinone oxidoreductase / zeta-crystallin signature. Belongs to the zinc-containing alcohol dehydrogenase family, quinone oxidoreductase subfamily. P96826 No intermediary metabolism and respiration ML2638 MMAR_0361 MSMEG_0097 Mb0154 NC_000962.3 Mycobrowser_v2 CDS 176665 176952 . - 0 Rv0150c Rv0150c Function unknown Conserved hypothetical protein Rv0150c, (MTCI5.24c), len: 95 aa. Conserved hypothetical protein, showing some similarity with C-terminus of O53949|Rv1800|MTV049.22 PPE-family protein from Mycobacterium tuberculosis (655 aa), FASTA score: (36.5% identity in 104 aa overlap). P96827 No conserved hypotheticals Mb0155c NC_000962.3 Mycobrowser_v2 CDS 209703 210812 . - 0 Rv0179c lprO Unknown Possible lipoprotein LprO Rv0179c, (MTCI28.19c), len: 369 aa. Possible lprO, lipoprotein (visibly not conserved). Contains possible N-terminal signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O07423 No cell wall and cell processes ML2599 MMAR_0422 MSMEG_0210 Mb0185c NC_000962.3 Mycobrowser_v2 CDS 424269 424694 . - 0 Rv0354c PPE7 Function unknown PPE family protein PPE7 Rv0354c, (MTCY13E10.14c), len: 141 aa. PPE7, Member of the Mycobacterium tuberculosis PPE family, similar to others e.g. MTCY63_9 from Mycobacterium tuberculosis (2411 aa), FASTA scores: E(): 3.6e-11, (47.6% identity in 103 aa overlap). Possible continuation of ORF upstream, but no sequence error apparent. Q6MX45 No PE/PPE Mb0362c NC_000962.3 Mycobrowser_v2 CDS 177543 179309 . - 0 Rv0151c PE1 Function unknown PE family protein PE1 Rv0151c, (MTCI5.25c), len: 588 aa. PE1, Member of the Mycobacterium tuberculosis PE family (see citation below), with N-terminal region similar to others e.g. MTV032_2 PE_PGRS family from Mycobacterium tuberculosis (468 aa), FASTA scores: opt: 1125, E(): 0, (46.3% identity in 456 aa overlap); MTCY493_24 from Mycobacterium tuberculosis FASTA score: (42.5% identity in 558 aa overlap). Also similar to upstream ORF MTCI5.26c FASTA score: (54.7% identity in 464 aa overlap). Also shows similarity to C-terminal part of some PPE family proteins e.g. MTV049_21 from Mycobacterium tuberculosis FASTA score: (41.5% identity in 591 aa overlap). Q79G06 No PE/PPE MMAR_0372 Mb0156c NC_000962.3 Mycobrowser_v2 CDS 179319 180896 . - 0 Rv0152c PE2 Function unknown PE family protein PE2 Rv0152c, (MTCI5.26c), len: 525 aa. PE2, Member of the Mycobacterium tuberculosis PE family (see citation below), similar to ORF downstream Z92770|MTCI5_25 (588 aa), FASTA scores: opt: 1492, E(): 0, (54.7% identity in 464 aa overlap); and to many other PE family type members. Predicted to be an outer membrane protein (See Song et al., 2008). Q79G05 No PE/PPE Mb0157c NC_000962.3 Mycobrowser_v2 CDS 882524 882763 . + 0 Rv0787A Rv0787A Function unknown Conserved protein Rv0787A, len: 79 aa. Conserved protein, equivalent to MLCB5.24 hypothetical protein from Mycobacterium leprae (79 aa), FASTA scores: opt: 434, (84.8% identity in 79 aa overlap). Also similar to P12049|YEXA_BACSU hypothetical 9.7 kDa protein from Bacillus subtilis (84 aa), FASTA scores: opt: 172, E(): 4e-06, (44.4% identity in 72 aa overlap). Belongs to the UPF0062 family. Q8VKE0 No conserved hypotheticals ML2219A MMAR_4898 MSMEG_5832 Mb0811 NC_000962.3 Mycobrowser_v2 CDS 181155 181985 . - 0 Rv0153c ptbB Involved in signal transduction (via dephosphorylation). Can dephosphorylate in vitro the phosphotyrosine residue of myelin basic protein (MBP) at pH 7.0. Could be involved in virulence by interfering with phosphotyrosine-mediated signals in macrophages. Also able to dephosphorylate phosphoserine/threonine peptides and phosphoinositide substrates. [catalytic activity: protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate] Phosphotyrosine protein phosphatase PTPB (protein-tyrosine-phosphatase) (PTPase) Rv0153c, (MTCI5.27c), len: 276 aa. PtbB (alternate gene name: MPtpB), protein-tyrosine-phosphatase (see citation below), showing some similarity to several protein-tyrosine phosphatases, polyketide synthase and aminotransferase e.g. Q05918|IPHP_NOSCO|IPH protein-tyrosine-phosphatase precursor from Nostoc commune (294 aa), FASTA scores: opt: 150, E(): 0.0096, (26.8% identity in 269 aa overlap); etc. Supposedly a secreted protein. Potent and selective inhibitor is an isoxazole compound (See Seollner et al., 2007). P96830 No regulatory proteins MMAR_0373 MSMEG_0100 Mb0158c NC_000962.3 Mycobrowser_v2 CDS 181987 183198 . - 0 Rv0154c fadE2 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE2 Rv0154c, (MTCI5.28c), len: 403 aa. Probable fadE2, acyl-CoA dehydrogenase, similar to many e.g. C-terminal region of O01590 acyl-CoA dehydrogenase (974 aa), FASTA scores: opt: 1150, E(): 0, (50.0% identity in 402 aa overlap); ACDS_MEGEL|Q06319 acyl-CoA dehydrogenase (short-chain) (383 aa), FASTA score: (35.0% identity in 306 aa overlap). Could belong to the acyl-CoA dehydrogenases family. P96831 No lipid metabolism ML2637 Mb0159c NC_000962.3 Mycobrowser_v2 CDS 1593505 1593978 . + 0 Rv1419 Rv1419 Unknown Unknown protein Rv1419, (MTCY21B4.37), len: 157 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). P64849 No conserved hypotheticals MMAR_3986 Mb1454 NC_000962.3 Mycobrowser_v2 CDS 183622 184722 . + 0 Rv0155 pntAa The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [catalytic activity: NADPH + NAD+ = NADP+ + NADH]. Probable NAD(P) transhydrogenase (subunit alpha) PntAa [first part; catalytic part] (pyridine nucleotide transhydrogenase subunit alpha) (nicotinamide nucleotide transhydrogenase subunit alpha) Rv0155, (MTCI5.29), len: 366 aa. Probable pntAa, first part of NAD(P) transhydrogenase subunit alpha, similar to N-terminus of others e.g. PNTA_ECOLI|P07001|P76888|B1603 NAD (P) transhydrogenase subunit alpha from Escherichia coli strain K12 (510 aa), FASTA scores: opt: 921, E(): 0, (42.1% identity in 361 aa overlap); proton-translocating nicotinamide nucleotide transhydrogenase subunit PNTAA. P96832 No intermediary metabolism and respiration ML2636 MMAR_0377 MSMEG_0152 Mb0160 NC_000962.3 Mycobrowser_v2 CDS 210892 212250 . - 0 Rv0180c Rv0180c Unknown Probable conserved transmembrane protein Rv0180c, (MTCI28.20c), len: 452 aa. Probable conserved transmembrane protein, equivalent to CAC32132.1|AL583926 probable conserved membrane protein from Mycobacterium leprae (465 aa). Shows some similarity with others membrane proteins e.g. AL096849|SCI11_29 from Streptomyces coelicolor (354 aa), FASTA scores: opt: 190, E(): 0.00067, (25.9% identity in 409 aa overlap). O07424 No cell wall and cell processes ML2600 MMAR_0423 Mb0186c NC_000962.3 Mycobrowser_v2 RBS 212256 212260 . - 0 Rv0180c Rv0180c Unknown Probable conserved transmembrane protein Rv0180c, (MTCI28.20c), len: 452 aa. Probable conserved transmembrane protein, equivalent to CAC32132.1|AL583926 probable conserved membrane protein from Mycobacterium leprae (465 aa). Shows some similarity with others membrane proteins e.g. AL096849|SCI11_29 from Streptomyces coelicolor (354 aa), FASTA scores: opt: 190, E(): 0.00067, (25.9% identity in 409 aa overlap). O07424 No cell wall and cell processes ML2600 MMAR_0423 Mb0186c NC_000962.3 Mycobrowser_v2 CDS 224724 226571 . - 0 Rv0193c Rv0193c Unknown Hypothetical protein Rv0193c, (MTV033.01c-MTCI28.32), len: 615 aa. Hypothetical unknown protein. O07437 No conserved hypotheticals MMAR_5224 Mb0199c NC_000962.3 Mycobrowser_v2 CDS 184723 185055 . + 0 Rv0156 pntAb The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [catalytic activity: NADPH + NAD+ = NADP+ + NADH]. Probable NAD(P) transhydrogenase (subunit alpha) PntAb [second part; integral membrane protein] (pyridine nucleotide transhydrogenase subunit alpha) (nicotinamide nucleotide transhydrogenase subunit alpha) Rv0156, (MTCI5.30), len: 110 aa. Probable pntAb, second part of NAD(P) transhydrogenase subunit alpha, integral membrane protein, similar to C-terminus of others e.g. Q59764 nicotinamide nucleotide transhydrogenase subunit PNTAB (139 aa), FASTA scores: opt: 247, E(): 1.9e-11, (45.5% identity in 88 aa overlap). P96833 No intermediary metabolism and respiration ML2635 MMAR_0378 MSMEG_0151 Mb0161 NC_000962.3 Mycobrowser_v2 CDS 185052 186479 . + 0 Rv0157 pntB The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [catalytic activity: NADPH + NAD+ = NADP+ + NADH]. Probable NAD(P) transhydrogenase (subunit beta) PntB [integral membrane protein] (pyridine nucleotide transhydrogenase subunit beta) (nicotinamide nucleotide transhydrogenase subunit beta) Rv0157, (MTCI5.31), len: 475 aa. Probable pntB, pyridine nucleotide transhydrogenase (nicotinamide nucleotide transhydrogenase) subunit beta, integral membrane protein, similar to others e.g. Q59763 proton-translocating nicotinamide nucleotide transhydrogenase subunit beta from hodospirillum rubrum (464 aa), FASTA scores: opt: 1344, E(): 0, (46.4% identity in 472 aa overlap); P07002|PNTB_ECOLI|P76890|PNTB|B1602|Z2597|ECS2308 NAD(P) transhydrogenase subunit beta from Escherichia coli strains K12 and O157:H7 (462 aa). P96834 No intermediary metabolism and respiration ML2634 MMAR_0379 MSMEG_0150 Mb0162 NC_000962.3 Mycobrowser_v2 CDS 186785 187429 . + 0 Rv0158 Rv0158 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (possibly TetR-family) Rv0158, (MTV032.01), len: 214 aa. Probable transcriptional regulator, possibly TetR family, showing weak similarity to various transcriptional activators and repressors e.g. P32398|YIXD_BACSU|YHGD hypothetical transcriptional regulatory protein from Bacillus subtilis (191 aa), FASTA scores: opt:172, E(): 2.4e-05, (23.0% identity in 191 aa overlap). Contains helix-turn-helix motif at aa 32-53 (Score 1296, +3.60 SD). Could belong to the TetR/AcrR family of transcriptional regulators. O53641 No regulatory proteins ML2633 MMAR_0381 MSMEG_0120 Mb0163 NC_000962.3 Mycobrowser_v2 CDS 187433 188839 . - 0 Rv0159c PE3 Function unknown PE family protein PE3 Rv0159c, (MTV032.02c), len: 468 aa. PE3, Member of the Mycobacterium tuberculosis PE family (see citation below), similar to many other PE proteins e.g. O06828 from Mycobacterium tuberculosis (528 aa), FASTA scores: opt: 1163, E(): 0, (45.8% identity in 467 aa overlap). Also highly similar to upstream MTV032_3, and to MTCI5_25, MTCI5_26, MTV049_ 21, MTCY1A10_26, etc. Q79G04 No PE/PPE MMAR_0383 Mb0164c NC_000962.3 Mycobrowser_v2 RBS 188845 188849 . - 0 Rv0159c PE3 Function unknown PE family protein PE3 Rv0159c, (MTV032.02c), len: 468 aa. PE3, Member of the Mycobacterium tuberculosis PE family (see citation below), similar to many other PE proteins e.g. O06828 from Mycobacterium tuberculosis (528 aa), FASTA scores: opt: 1163, E(): 0, (45.8% identity in 467 aa overlap). Also highly similar to upstream MTV032_3, and to MTCI5_25, MTCI5_26, MTV049_ 21, MTCY1A10_26, etc. Q79G04 No PE/PPE MMAR_0383 Mb0164c NC_000962.3 Mycobrowser_v2 CDS 188931 190439 . - 0 Rv0160c PE4 Function unknown PE family protein PE4 Rv0160c, (MTV032.03c), len: 502 aa. PE4, Member of the Mycobacterium tuberculosis PE family (see citation below), similar to many other PE proteins e.g. Z92770|MTCI5_26c from Mycobacterium tuberculosis (525 aa), FASTA scores: opt: 816, E(): 0, (41.4% identity in 367 aa overlap); C-terminal region of O06801|RV1768|MTCY28.34 from Mycobacterium tuberculosis (618 aa), FASTA scores: opt: 417, E(): 6.7e-18, (53.5% identity in 142 aa overlap). Also highly similar to downstream ORF MTV032_2. Q7DAC9 No PE/PPE ML2632 MMAR_0384 Mb0165c NC_000962.3 Mycobrowser_v2 RBS 190447 190451 . - 0 Rv0160c PE4 Function unknown PE family protein PE4 Rv0160c, (MTV032.03c), len: 502 aa. PE4, Member of the Mycobacterium tuberculosis PE family (see citation below), similar to many other PE proteins e.g. Z92770|MTCI5_26c from Mycobacterium tuberculosis (525 aa), FASTA scores: opt: 816, E(): 0, (41.4% identity in 367 aa overlap); C-terminal region of O06801|RV1768|MTCY28.34 from Mycobacterium tuberculosis (618 aa), FASTA scores: opt: 417, E(): 6.7e-18, (53.5% identity in 142 aa overlap). Also highly similar to downstream ORF MTV032_2. Q7DAC9 No PE/PPE ML2632 MMAR_0384 Mb0165c NC_000962.3 Mycobrowser_v2 CDS 212277 213011 . - 0 Rv0181c Rv0181c Function unknown Conserved hypothetical protein Rv0181c, (MTCI28.21c), len: 244 aa. Conserved hypothetical protein, highly similar to other hypothetical proteins e.g. YHHW_ECOLI|P46852 hypothetical 26.3 kd protein from Escherichia coli (231 aa), FASTA scores: opt: 479, E(): 1.2e-29, (37.3% identity in 233 aa overlap); P73623|SLL1773 hypothetical 25.7 kDa protein from Synechocystis sp. strain PCC 6803 (232 aa), FASTA score: (39.1% identity in 233 aa overlap). P65724 No conserved hypotheticals ML2601 MMAR_0424 MSMEG_0215 Mb0187c NC_000962.3 Mycobrowser_v2 CDS 190607 191956 . + 0 Rv0161 Rv0161 Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0161, (MTCI28.01, MTV032.04), len: 449 aa. Possible oxidoreductase, similar to hypothetical proteins and various oxidoreductases e.g. AIP2_YEAST|P46681 actin interacting protein 2 (530 aa), FASTA scores: opt: 356, E (): 0, (33.3% identity in 357 aa overlap); DLD1_YEAST|P32891 d-lactate dehydrogenase (cytochrome) (587 aa), FASTA scores: opt: 311, E(): 2.5e-20, (27.9% identity in 366 aa overlap). Also similar to other Mycobacteria proteins e.g. MTCY339.30c from Mycobacterium tuberculosis FASTA score: (29.4% identity in 357 aa overlap); MLCL622.30c from Mycobacterium tuberculosis (449 aa). O07406 No intermediary metabolism and respiration ML2631 MMAR_0388 MSMEG_5045 Mb0166 NC_000962.3 Mycobrowser_v2 CDS 191984 193135 . - 0 Rv0162c adhE1 Dehydrogeneses a alcohol (OXIDO-reduction) [catalytic activity: an alcohol + NAD+ = an aldehyde or ketone + NADH]. Probable zinc-type alcohol dehydrogenase (E subunit) AdhE1 Rv0162c, (MTCI28.02c), len: 383 aa. Probable adhE1, zinc-type alcohol dehydrogenase, similar to others e.g. ADH_MACMU|P28469 alcohol dehydrogenase alpha chain (374 aa), FASTA scores: opt: 619, E(): 0, (34.7% identity in 363 aa overlap). Also similar to other alcohol dehydrogenases from Mycobacterium tuberculosis e.g. MTCY369.06c FASTA score: (34.0% identity in 365 aa overlap), MTV022_9 FASTA score: (35.0% identity in 371 aa overlap). Contains PS00059 Zinc-containing alcohol dehydrogenases signature. Belongs to the zinc-containing alcohol dehydrogenase family, class-I subfamily. Cofactor: zinc. Q7DAC8 No intermediary metabolism and respiration MMAR_0405 MSMEG_0127 Mb0167c NC_000962.3 Mycobrowser_v2 RBS 193144 193148 . - 0 Rv0162c adhE1 Dehydrogeneses a alcohol (OXIDO-reduction) [catalytic activity: an alcohol + NAD+ = an aldehyde or ketone + NADH]. Probable zinc-type alcohol dehydrogenase (E subunit) AdhE1 Rv0162c, (MTCI28.02c), len: 383 aa. Probable adhE1, zinc-type alcohol dehydrogenase, similar to others e.g. ADH_MACMU|P28469 alcohol dehydrogenase alpha chain (374 aa), FASTA scores: opt: 619, E(): 0, (34.7% identity in 363 aa overlap). Also similar to other alcohol dehydrogenases from Mycobacterium tuberculosis e.g. MTCY369.06c FASTA score: (34.0% identity in 365 aa overlap), MTV022_9 FASTA score: (35.0% identity in 371 aa overlap). Contains PS00059 Zinc-containing alcohol dehydrogenases signature. Belongs to the zinc-containing alcohol dehydrogenase family, class-I subfamily. Cofactor: zinc. Q7DAC8 No intermediary metabolism and respiration MMAR_0405 MSMEG_0127 Mb0167c NC_000962.3 Mycobrowser_v2 RBS 3406275 3406278 . + 0 Rv0162c adhE1 Dehydrogeneses a alcohol (OXIDO-reduction) [catalytic activity: an alcohol + NAD+ = an aldehyde or ketone + NADH]. Probable zinc-type alcohol dehydrogenase (E subunit) AdhE1 Rv0162c, (MTCI28.02c), len: 383 aa. Probable adhE1, zinc-type alcohol dehydrogenase, similar to others e.g. ADH_MACMU|P28469 alcohol dehydrogenase alpha chain (374 aa), FASTA scores: opt: 619, E(): 0, (34.7% identity in 363 aa overlap). Also similar to other alcohol dehydrogenases from Mycobacterium tuberculosis e.g. MTCY369.06c FASTA score: (34.0% identity in 365 aa overlap), MTV022_9 FASTA score: (35.0% identity in 371 aa overlap). Contains PS00059 Zinc-containing alcohol dehydrogenases signature. Belongs to the zinc-containing alcohol dehydrogenase family, class-I subfamily. Cofactor: zinc. Q7DAC8 No intermediary metabolism and respiration MMAR_0405 MSMEG_0127 Mb0167c NC_000962.3 Mycobrowser_v2 CDS 193117 193572 . + 0 Rv0163 Rv0163 Function unknown Conserved protein Rv0163, (MTCI28.03), len: 151 aa. Conserved protein, similar to others e.g. Q44017 hypothetical 16.6 KDA protein in GBD 5'region (ORF6)from Alcaligenes eutrophus (145 aa), FASTA scores: opt: 155, E(): 0.0002, (26.6% identity in 139 aa overlap). Also weak similarity with MTV008.31c|Rv2475c|B70867 from Mycobacterium tuberculosis (138 aa). O07408 No conserved hypotheticals MMAR_0406 MSMEG_0128 Mb0168 NC_000962.3 Mycobrowser_v2 CDS 193626 194111 . + 0 Rv0164 TB18.5 Function unknown Conserved protein TB18.5 Rv0164, (MTCI28.04), len: 161 aa. TB18.5, conserved protein, equivalent to CAB08818.1|Z95398 hypothetical protein from Mycobacterium leprae (156 aa) FASTA scores: opt: 762, E(): 0, (76.3% identity in 152 aa overlap). Some similarity to Rv2185c, Rv0854, Rv0857 from Mycobacterium tuberculosis. Alternative start codon has been suggested. 3' part corrected since first submission (-24 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). Q8VKQ8 No conserved hypotheticals ML2629 MMAR_0407 MSMEG_0129 Mb0169 NC_000962.3 Mycobrowser_v2 CDS 704752 705909 . - 0 Rv0610c Rv0610c Unknown Hypothetical protein Rv0610c, (MTCY19H5.11), len: 385 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07767 No conserved hypotheticals Mb0627c NC_000962.3 Mycobrowser_v2 CDS 194144 194815 . - 0 Rv0165c mce1R Involved in transcriptional mechanism Probable transcriptional regulatory protein Mce1R (probably GntR-family) Rv0165c, (MTCI28.05c), len: 223 aa. Probable mce1R, transcriptional regulator, GntR family (See Casali et al., 2006), showing some similarity to several e.g. NTRA_CHELE|P54988 nta operon transcriptional regulator (231 aa), FASTA scores: opt: 154, E(): 0.00058, (32.0% identity in 125 aa overlap); P46833|GNTR_BACLI gluconate operon transcriptional repressor from Bacillus licheniformis (243 aa); GNTR_BACSU gluconate operon repressor from Bacillus subtilis (243 aa). Also similar to Rv0043c from Mycobacterium tuberculosis. Seems to belong to the GntR family of transcriptional regulators. Start changed since first submission (-41 aa). Q79G00 No regulatory proteins MMAR_0408 MSMEG_1995 Mb0170c,Mb0171c NC_000962.3 Mycobrowser_v2 RBS 194946 194948 . - 0 Rv0165c mce1R Involved in transcriptional mechanism Probable transcriptional regulatory protein Mce1R (probably GntR-family) Rv0165c, (MTCI28.05c), len: 223 aa. Probable mce1R, transcriptional regulator, GntR family (See Casali et al., 2006), showing some similarity to several e.g. NTRA_CHELE|P54988 nta operon transcriptional regulator (231 aa), FASTA scores: opt: 154, E(): 0.00058, (32.0% identity in 125 aa overlap); P46833|GNTR_BACLI gluconate operon transcriptional repressor from Bacillus licheniformis (243 aa); GNTR_BACSU gluconate operon repressor from Bacillus subtilis (243 aa). Also similar to Rv0043c from Mycobacterium tuberculosis. Seems to belong to the GntR family of transcriptional regulators. Start changed since first submission (-41 aa). Q79G00 No regulatory proteins MMAR_0408 MSMEG_1995 Mb0170c,Mb0171c NC_000962.3 Mycobrowser_v2 CDS 475350 475742 . + 0 Rv0396 Rv0396 Unknown Hypothetical protein Rv0396, (MTCY04D9.08), len: 130 aa. Hypothetical unknown protein. P95204 No conserved hypotheticals Mb0402 NC_000962.3 Mycobrowser_v2 RBS 194980 194984 . + 0 Rv0166 fadD5 Function unknown, but involved in lipid degradation. Probable fatty-acid-CoA ligase FadD5 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv0166, (MTCI28.06), len: 554 aa. Probable fadD5, fatty-acid-CoA synthetase, similar to many eg LCFA_ECOLI|P29212 long-chain-fatty-acid--CoA ligase (561 aa), FASTA scores: opt: 612, E(): 0, (29.4% identity in 534 aa overlap). Also similar to many other fatty-acid-CoA ligases from Mycobacterium tuberculosis e.g. MTCY07A7.11c FASTA score: (35.3% identity in 487 aa overlap), MTV013_10, MTY25D10_30, etc. Contains PS00455 putative AMP-binding domain signature. O07411 No lipid metabolism ML2586 MMAR_0409 MSMEG_0131 Mb0172 NC_000962.3 Mycobrowser_v2 CDS 194993 196657 . + 0 Rv0166 fadD5 Function unknown, but involved in lipid degradation. Probable fatty-acid-CoA ligase FadD5 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv0166, (MTCI28.06), len: 554 aa. Probable fadD5, fatty-acid-CoA synthetase, similar to many eg LCFA_ECOLI|P29212 long-chain-fatty-acid--CoA ligase (561 aa), FASTA scores: opt: 612, E(): 0, (29.4% identity in 534 aa overlap). Also similar to many other fatty-acid-CoA ligases from Mycobacterium tuberculosis e.g. MTCY07A7.11c FASTA score: (35.3% identity in 487 aa overlap), MTV013_10, MTY25D10_30, etc. Contains PS00455 putative AMP-binding domain signature. O07411 No lipid metabolism ML2586 MMAR_0409 MSMEG_0131 Mb0172 NC_000962.3 Mycobrowser_v2 RBS 382480 382485 . + 0 Rv0313 Rv0313 Function unknown Conserved protein Rv0313, (MTCY63.18), len: 128 aa. Conserved protein, equivalent only to CAC32049.1|AL583926 conserved hypothetical protein from Mycobacterium leprae (130 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O07240 No conserved hypotheticals ML2518 MMAR_0564 MSMEG_3685 Mb0321 NC_000962.3 Mycobrowser_v2 CDS 382490 382876 . + 0 Rv0313 Rv0313 Function unknown Conserved protein Rv0313, (MTCY63.18), len: 128 aa. Conserved protein, equivalent only to CAC32049.1|AL583926 conserved hypothetical protein from Mycobacterium leprae (130 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O07240 No conserved hypotheticals ML2518 MMAR_0564 MSMEG_3685 Mb0321 NC_000962.3 Mycobrowser_v2 CDS 985513 985971 . - 0 Rv0887c Rv0887c Function unknown Conserved hypothetical protein Rv0887c, (MTCY31.15c), len: 152 aa. Conserved hypothetical protein, highly similar to others e.g. NP_436346.1|NC_003037 Hypothetical protein from Sinorhizobium meliloti (149 aa); AL132644|SCI8_26 hypothetical protein from Streptomyces coelicolor (194 aa), FASTA scores: opt: 220, E(): 1.5e-07, (33.6% identity in 131 aa overlap); etc. Also shows weak similarity with transposases and related proteins. P64741 No conserved hypotheticals ML2133 MMAR_4645 MSMEG_5680 Mb0911c NC_000962.3 Mycobrowser_v2 RBS 985977 985982 . - 0 Rv0887c Rv0887c Function unknown Conserved hypothetical protein Rv0887c, (MTCY31.15c), len: 152 aa. Conserved hypothetical protein, highly similar to others e.g. NP_436346.1|NC_003037 Hypothetical protein from Sinorhizobium meliloti (149 aa); AL132644|SCI8_26 hypothetical protein from Streptomyces coelicolor (194 aa), FASTA scores: opt: 220, E(): 1.5e-07, (33.6% identity in 131 aa overlap); etc. Also shows weak similarity with transposases and related proteins. P64741 No conserved hypotheticals ML2133 MMAR_4645 MSMEG_5680 Mb0911c NC_000962.3 Mycobrowser_v2 RBS 196852 196855 . + 0 Rv0167 yrbE1A Unknown Conserved integral membrane protein YrbE1A Rv0167, (MTCI28.07), len: 265 aa. YrbE1A, unknown integral membrane protein, part of mce1 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07791|Rv0587|MTCY19H5.35|yrbE2A (265 aa); O53965|Rv1964|MTV051.02|yrbE3A (265 aa); etc. Also highly similar or similar to conserved hypothetical integral membrane proteins of yrbEA type, e.g. NP_302654.1|NC_002677 conserved membrane protein from Mycobacterium leprae (267 aa); P45030|YRBE_HAEIN|HI1086 hypothetical protein from Haemophilus influenzae (261 aa), FASTA scores: opt: 328, E(): 1.8e-15, (26.6% identity in 244 aa overlap); etc. O07412 No virulence, detoxification, adaptation ML2587 MMAR_0410 MSMEG_0132 Mb0173 NC_000962.3 Mycobrowser_v2 CDS 196861 197658 . + 0 Rv0167 yrbE1A Unknown Conserved integral membrane protein YrbE1A Rv0167, (MTCI28.07), len: 265 aa. YrbE1A, unknown integral membrane protein, part of mce1 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07791|Rv0587|MTCY19H5.35|yrbE2A (265 aa); O53965|Rv1964|MTV051.02|yrbE3A (265 aa); etc. Also highly similar or similar to conserved hypothetical integral membrane proteins of yrbEA type, e.g. NP_302654.1|NC_002677 conserved membrane protein from Mycobacterium leprae (267 aa); P45030|YRBE_HAEIN|HI1086 hypothetical protein from Haemophilus influenzae (261 aa), FASTA scores: opt: 328, E(): 1.8e-15, (26.6% identity in 244 aa overlap); etc. O07412 No virulence, detoxification, adaptation ML2587 MMAR_0410 MSMEG_0132 Mb0173 NC_000962.3 Mycobrowser_v2 CDS 197660 198529 . + 0 Rv0168 yrbE1B Unknown Conserved integral membrane protein YrbE1B Rv0168, (MTCI28.08), len: 289 aa. YrbE1B, unknown integral membrane protein, part of mce1 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07790|Rv0588|MTCY19H5.34|yrbE2B (295 aa); O53966|Rv1965|MTV051.03|yrbE3B (271 aa); etc. Also highly similar to conserved hypothetical integral membrane proteins of the yrbEB type, e.g. NP_302655.1|NC_002677 conserved membrane protein from Mycobacterium leprae (289 aa); P45030|YRBE_HAEIN|HI1086 hypothetical protein from Haemophilus influenzae (261 aa), FASTA scores: opt: 223, E(): 7.6e-07, (23.7% identity in 257 aa overlap); etc. O07413 No virulence, detoxification, adaptation ML2588 MMAR_0411 MSMEG_0133 Mb0174 NC_000962.3 Mycobrowser_v2 CDS 198534 199898 . + 0 Rv0169 mce1A Unknown, but thought to be involved in host cell invasion (entry and survival inside macrophages). Mce-family protein Mce1A Rv0169, (MTCI28.09), len: 454 aa. Mce1A; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07789|MCE2|Rv0589|MTCY19H5.33c|mce2A (404 aa); O53967|MCE3|Rv1966|MTV051.04|mce3A (425 aa); etc. Also highly similar to others e.g. AAD52105.1|AF113402_1|AF113402 mycobacterial cell entry protein from Mycobacterium bovis BCG (454 aa); NP_302656.1|NC_002677 putative cell invasion protein from Mycobacterium leprae (441 aa); AAA92845.1|U26018 mce gene product from Mycobacterium avium (88 aa) (similarity on C-terminus); CAC12798.1|AL445327 putative secreted protein from Streptomyces coelicolor (418 aa); etc. Note that equivalent, but longer 22 aa, to P72013|CAA50257.1|X70901 Mcep protein from Mycobacterium tuberculosis (432 aa). Contains a very hydrophobic region around residues 20-35. Note that previously known as mce1. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). Q79FZ9 No virulence, detoxification, adaptation ML2589 Mb0175 NC_000962.3 Mycobrowser_v2 CDS 199895 200935 . + 0 Rv0170 mce1B Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce1B Rv0170, (MTCI28.10), len: 346 aa. Mce1B (alternate gene name: mceD); belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07788|Rv0590|MTCY19H5.32c|mce2B (275 aa); O53968|Rv1967|MTV051.05|mce3B (342 aa); etc. Also highly similar to others e.g. NP_302657.1|NC_002677 putative secreted protein from Mycobacterium leprae (346 aa); CAC12797.1|AL445327 putative secreted protein from Streptomyces coelicolor (354 aa); etc. Contains hydrophobic region in N-terminal 30 residues. In Escherichia coli, N-terminal part is functional and directs export of a leaderless beta-lactamase into the periplasm (see Chubb et al., 1998). Predicted to be an outer membrane protein (See Song et al., 2008). O07414 No virulence, detoxification, adaptation ML2590 MMAR_0413 Mb0176 NC_000962.3 Mycobrowser_v2 CDS 213028 214140 . - 0 Rv0182c sigG The sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released. Probable alternative RNA polymerase sigma factor SigG (RNA polymerase ECF type sigma factor) Rv0182c, (MTCI28.22c), len: 370 aa (start site uncertain; first of several possibles was chosen, but note that this overlaps the upstream ORF). Probable sigG, alternative RNA polymerase sigma subunit (see citations below), similar to many e.g. Q45585|SIGW_BACSU RNA polymerase sigma factor from Bacillus subtilis (187 aa). Also similar to nine other ECF sigma factors from Mycobacterium tuberculosis e.g. Rv1221, Rv0735, etc. Contains PS01063 Sigma-70 factors ECF subfamily signature and probable helix-turn helix motif from aa 205-226 (Score 1181, +3.21 SD). Belongs to the sigma-70 factor family, ECF subfamily. O07426 No information pathways ML2602 MMAR_0426 MSMEG_0219 Mb0188c NC_000962.3 Mycobrowser_v2 CDS 200932 202479 . + 0 Rv0171 mce1C Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce1C Rv0171, (MTCI28.11), len: 515 aa. Mce1C; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07787|Rv0591|MTCY19H5.31|mce2C (481 aa); O53969|Rv1968|MTV051.06|mce3C (410 aa); etc. Also highly similar to others e.g. NP_302658.1|NC_002677 putative secreted protein from Mycobacterium leprae (519 aa); CAC12796.1|AL445327 putative secreted protein from Streptomyces coelicolor (351 aa); etc. Weakly similar to downstream ORF Rv0172|MTCI28.12|mce1D (530 aa), FASTA score: (24.6% identity in 552 aa overlap). Contains possible signal sequence and highly proline-rich C-terminus. Predicted to be an outer membrane protein (See Song et al., 2008). O07415 No virulence, detoxification, adaptation ML2591 MMAR_0414 MSMEG_0136 Mb0177 NC_000962.3 Mycobrowser_v2 RBS 219474 219478 . + 0 Rv0188 Rv0188 Unknown Probable conserved transmembrane protein Rv0188, (MTCI28.27), len: 143 aa. Probable conserved transmembrane protein, similar to T35347|4835334|CAB42956.1|AL049863|SC5H1_31 probable membrane protein from Streptomyces coelicolor (147 aa), FASTA scores: opt: 326, E(): 6.5e-15, (36.2% identity in 141 aa overlap); N-terminus of P80185|MTRC_METTH tetrahydromethanopterin S-methyltransferase subunit C from Methanobacterium thermoautotrophicum strain Marburg/DSM 2133 (266 aa), FASTA scores: opt: 125, E(): 0.033, (31.6% identity in 98 aa overlap). Also similar to Rv3635 from Mycobacterium tuberculosis. O07432 No cell wall and cell processes Mb0194 NC_000962.3 Mycobrowser_v2 CDS 219486 219917 . + 0 Rv0188 Rv0188 Unknown Probable conserved transmembrane protein Rv0188, (MTCI28.27), len: 143 aa. Probable conserved transmembrane protein, similar to T35347|4835334|CAB42956.1|AL049863|SC5H1_31 probable membrane protein from Streptomyces coelicolor (147 aa), FASTA scores: opt: 326, E(): 6.5e-15, (36.2% identity in 141 aa overlap); N-terminus of P80185|MTRC_METTH tetrahydromethanopterin S-methyltransferase subunit C from Methanobacterium thermoautotrophicum strain Marburg/DSM 2133 (266 aa), FASTA scores: opt: 125, E(): 0.033, (31.6% identity in 98 aa overlap). Also similar to Rv3635 from Mycobacterium tuberculosis. O07432 No cell wall and cell processes Mb0194 NC_000962.3 Mycobrowser_v2 CDS 202476 204068 . + 0 Rv0172 mce1D Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce1D Rv0172, (MTCI28.12), len: 530 aa. Mce1D; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07786|Rv0592|MTCY19H5.30c|mce2D (508 aa); O53970|Rv1969|MTV051.07|mce3D (423 aa); etc. Also highly similar to others e.g. NP_302659.1|NC_002677 putative secreted protein from Mycobacterium leprae (531 aa); CAC12795.1|AL445327 putative secreted protein from Streptomyces coelicolor (337 aa); etc. Hydrophobic region at N-terminus. Predicted to be an outer membrane protein (See Song et al., 2008). O07416 No virulence, detoxification, adaptation ML2592 MMAR_0415 MSMEG_0137 Mb0178 NC_000962.3 Mycobrowser_v2 CDS 204065 205237 . + 0 Rv0173 lprK Unknown, but thought to be involved in host cell invasion. Possible Mce-family lipoprotein LprK (Mce-family lipoprotein Mce1E) Rv0173, (MTCI28.13), len: 390 aa. Possible lprK (alternate gene name: mce1E), lipoprotein which belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07785|LPRL|Rv0593|MTCY19H5.29|mce2E (402 aa); O53971|LPRM|Rv1970|MTV051.08|mce3E (377 aa); etc. Also highly similar to others e.g. NP_302660.1|NC_002677 putative lipoprotein from Mycobacterium leprae (392 aa); CAC12794.1|AL445327 putative secreted protein from Streptomyces coelicolor (413 aa); etc. Contains PS00013 prokaryotic membrane lipoprotein lipid attachment site. O07417 No cell wall and cell processes ML2593 MMAR_0416 MSMEG_0138 Mb0179 NC_000962.3 Mycobrowser_v2 CDS 214088 214927 . + 0 Rv0183 Rv0183 Function unknown; probably involved in cellular metabolism. Possible lysophospholipase Rv0183, (MTCI28.23), len: 279 aa. Possible lysophospholipase, similar to several (especially eukaryotic enzymes, weaker with Escherichia coli), e.g. U67963|HSU67963_1 Human lysophospholipase homolog from Homo sapiens (313 aa), FASTA scores: opt: 569, E(): 2.6e-29, (37.1% identity in 259 aa overlap); P07000|PLDB_ECOLI lysophospholipase L2 from Escherichia coli (165 aa), FASTA scores: opt: 219, E(): 0.00012. Start changed based on similarity to AE001997_8 from Deinococcus radiodurans (282 aa), FASTA scores: opt: 510, E(): 1.4e-25, (34.8% identity in 282 aa overlap). Also shows some similarity to epoxide hydrolases from Mycobacterium tuberculosis e.g. Rv1938 FASTA score: (30.7% identity in 114 aa overlap); and O07214|YR15_MYCTU|Rv2715|MT2788|MTCY05A6.36 (341 aa). O07427 No intermediary metabolism and respiration ML2603 MSMEG_0220 Mb0189 NC_000962.3 Mycobrowser_v2 RBS 205216 205219 . + 0 Rv0174 mce1F Unknown, but thought involved in host cell invasion. Mce-family protein Mce1F Rv0174, (MTCI28.14), len: 515 aa. Mce1F; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), similar to Mycobacterium tuberculosis proteins O07784|Rv0594|MTCY19H5.28c|mce2F (516 aa); O53972|Rv1971|MTV051.09|mce3F (437 aa); etc. Also highly similar to others e.g. NP_302661.1|NC_002677 putative secreted protein from Mycobacterium leprae (516 aa); AAF74993.1|AF143400_1|AF143400|996A027a protein from Mycobacterium avium (80 aa) (similarity on C-terminus); CAC12793.1|AL445327 putative secreted protein from Streptomyces coelicolor (433 aa); etc. Has hydrophobic stretch, possibly a signal peptide at the N-terminus. Predicted to be an outer membrane protein (See Song et al., 2008). O07418 No virulence, detoxification, adaptation ML2594 MMAR_0417 MSMEG_0139 Mb0180 NC_000962.3 Mycobrowser_v2 CDS 205231 206778 . + 0 Rv0174 mce1F Unknown, but thought involved in host cell invasion. Mce-family protein Mce1F Rv0174, (MTCI28.14), len: 515 aa. Mce1F; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), similar to Mycobacterium tuberculosis proteins O07784|Rv0594|MTCY19H5.28c|mce2F (516 aa); O53972|Rv1971|MTV051.09|mce3F (437 aa); etc. Also highly similar to others e.g. NP_302661.1|NC_002677 putative secreted protein from Mycobacterium leprae (516 aa); AAF74993.1|AF143400_1|AF143400|996A027a protein from Mycobacterium avium (80 aa) (similarity on C-terminus); CAC12793.1|AL445327 putative secreted protein from Streptomyces coelicolor (433 aa); etc. Has hydrophobic stretch, possibly a signal peptide at the N-terminus. Predicted to be an outer membrane protein (See Song et al., 2008). O07418 No virulence, detoxification, adaptation ML2594 MMAR_0417 MSMEG_0139 Mb0180 NC_000962.3 Mycobrowser_v2 CDS 265507 266295 . + 0 Rv0222 echA1 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Probable enoyl-CoA hydratase EchA1 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv0222, (MTCY08D5.17), len: 262 aa. Probable echA1, enoyl-CoA hydratase, similar to others e.g. AAC77915.1|AF063588 enoyl CoA hydratase from Rhodococcus fascians (275 aa); etc. P96404 No lipid metabolism MMAR_0465 MSMEG_2227 NC_000962.3 Mycobrowser_v2 CDS 206814 207455 . + 0 Rv0175 Rv0175 Unknown Probable conserved Mce associated membrane protein Rv0175, (MTCI28.15), len: 213 aa. Probable conserved Mce-associated membrane protein, equivalent, but longer in N-terminus, to CAC32127.1|AL583926 possible membrane protein from Mycobacterium leprae (182 aa). Also similar to mce-associated proteins from Mycobacterium tuberculosis e.g. Rv1363c, Rv0177, Rv1973, etc. Contains two 12 residue direct repeats at N-terminus. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O07419 No cell wall and cell processes ML2595 MMAR_0418 MSMEG_0140 Mb0181 NC_000962.3 Mycobrowser_v2 CDS 207452 208420 . + 0 Rv0176 Rv0176 Unknown Probable conserved Mce associated transmembrane protein Rv0176, (MTCI28.16), len: 322 aa. Probable conserved Mce-associated transmembrane protein. Contains short region of similarity to PRA_MYCLE|P41484 proline-rich antigen (36 kDa antigen) from Mycobacterium leprae (249 aa) (outside the proline-rich region), FASTA scores: opt: 165, E(): 2.9e-05, (40.0% identity in 65 aa overlap). Also similar to mce-associated proteins from Mycobacterium tuberculosis e.g. Rv1363c, Rv0177, Rv3493c, etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O07420 No cell wall and cell processes ML2596 MMAR_0419 MSMEG_0141 Mb0182 NC_000962.3 Mycobrowser_v2 CDS 705961 706344 . - 0 Rv0611c Rv0611c Unknown Hypothetical protein Rv0611c, (MTCY19H5.10), len: 127 aa. Hypothetical unknown protein. Note that first start has been taken although this overlaps slightly with the upstream ORF. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07766 No conserved hypotheticals Mb0628c NC_000962.3 Mycobrowser_v2 CDS 208938 209672 . + 0 Rv0178 Rv0178 Unknown Probable conserved Mce associated membrane protein Rv0178, (MTCI28.18), len: 244 aa. Probable conserved Mce-associated membrane protein, highly similar in C-terminus to CAC32130.1|AL583926 putative secreted protein from Mycobacterium leprae (184 aa). Also similar to mce-associated proteins from Mycobacterium tuberculosis e.g. Rv1363c, Rv0177, Rv1973, etc. Note that there is a 10 aa overlap with the upstream ORF. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O07422 No cell wall and cell processes ML2598 MMAR_0421 Mb0184 NC_000962.3 Mycobrowser_v2 CDS 930953 931597 . + 0 Rv0835 lpqQ Unknown Possible lipoprotein LpqQ Rv0835, (MTV043.27), len: 214 aa. Possible lpqQ, lipoprotein. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O53846 No cell wall and cell processes MMAR_1362 Mb0858 NC_000962.3 Mycobrowser_v2 CDS 516895 517803 . - 0 Rv0428c Rv0428c Acetylation, substrate unknown GCN5-related N-acetyltransferase Rv0428c, (MTCY22G10.25c), len: 302 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain in C-terminal part. See Vetting et al. 2005. P96274 No intermediary metabolism and respiration ML1930 Mb0436c NC_000962.3 Mycobrowser_v2 CDS 932279 932932 . - 0 Rv0836c Rv0836c Unknown Hypothetical protein Rv0836c, (MTV043.29c), len: 217 aa (start uncertain). Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53848 No conserved hypotheticals Mb0859c NC_000962.3 Mycobrowser_v2 CDS 214969 215718 . + 0 Rv0184 Rv0184 Function unknown Conserved hypothetical protein Rv0184, (MTCI28.24), len: 249 aa. Conserved hypothetical protein, equivalent to CAC32136.1|AL583926 conserved hypothetical protein from Mycobacterium leprae (249 aa); and C-terminus highly similar to CAB08793.1|Z95398 conserved hypothetical protein from Mycobacterium leprae (145 aa), FASTA scores: E(): 0, (75.2 identity in 145 aa overlap). Also similar to 049841|SCE9_39|T36358 hypothetical protein from Streptomyces coelicolor (418 aa), FASTA scores: opt: 231, E(): 8.1e-08, (30.4% identity in 270 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O07428 No conserved hypotheticals ML2604 MMAR_0428 MSMEG_0222 Mb0190 NC_000962.3 Mycobrowser_v2 CDS 215715 216224 . + 0 Rv0185 Rv0185 Function unknown; probably involved in a cellular metabolism. Conserved hypothetical protein Rv0185, (MTCI28.25a), len: 169 aa. Conserved hypothetical protein, equivalent to CAB08794.1|Z95398|MLCL622_2 from Mycobacterium leprae (168 aa), FASTA scores: opt: 861, E(): 0, (76.4% identity in 165 aa overlap). Contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O07429 No conserved hypotheticals ML2605 MMAR_0429 MSMEG_0223 Mb0191 NC_000962.3 Mycobrowser_v2 RBS 216260 216263 . + 0 Rv0185 Rv0185 Function unknown; probably involved in a cellular metabolism. Conserved hypothetical protein Rv0185, (MTCI28.25a), len: 169 aa. Conserved hypothetical protein, equivalent to CAB08794.1|Z95398|MLCL622_2 from Mycobacterium leprae (168 aa), FASTA scores: opt: 861, E(): 0, (76.4% identity in 165 aa overlap). Contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O07429 No conserved hypotheticals ML2605 MMAR_0429 MSMEG_0223 Mb0191 NC_000962.3 Mycobrowser_v2 CDS 216269 218344 . + 0 Rv0186 bglS Possibly involved in degradation [catalytic activity: hydrolysis of terminal, non-reducing beta-D-glucose residues with release of beta-D-glucose]. Probable beta-glucosidase BglS (gentiobiase) (cellobiase) (beta-D-glucoside glucohydrolase) Rv0186, (MTCI28.25b), len: 691 aa. Probable bglS, beta-glucosidase, highly similar to many e.g. BGLS_AGRTU|P27034 beta-glucosidase from Agrobacterium tumefaciens (818 aa), FASTA scores: opt: 643, E(): 0, (32.5% identity in 842 aa overlap). Seems to belong to family 3 of glycosyl hydrolases. O07430 No intermediary metabolism and respiration ML2606 MMAR_0430 Mb0192 NC_000962.3 Mycobrowser_v2 CDS 218390 218551 . - 0 Rv0186A mymT Coordinates Cu(I) ions into a Cu(I)-thiolate core, protects cell from copper toxicity Metallothionein, MymT Rv0186A, len: 53 aa. MymT, metallothionein, equivalent to MAV_4993|A0QMH5 hypothetical protein from Mycobacterium avium (strain 104) (51 aa), and MAP_3626c|Q73TU2 hypothetical protein from Mycobacterium avium subsp. paratuberculosis (51 aa), FASTA scores: opt: 312, E(): 4.6e-17, (81.2% identity in 48 aa overlap). P0CI28 No virulence, detoxification, adaptation NC_000962.3 Mycobrowser_v2 CDS 218705 219367 . + 0 Rv0187 Rv0187 Thought to be involved in transfer of methyl group. Probable O-methyltransferase Rv0187, (MTCI28.26), len: 220 aa. Probable O-methyltransferase, similar to many e.g. AB93458.1|AL357591 putative O-methyltransferase from Streptomyces coelicolor (223 aa); MDMC_STRMY|Q00719 O-methyltransferase from Streptomyces mycarofaciens (221 aa), FASTA scores: opt: 327, E(): 2.4e-17, (35.9% identity in 192 aa overlap). Also similar to Rv1703c, Rv1220c from Mycobacterium tuberculosis. O07431 No intermediary metabolism and respiration MSMEG_0224 Mb0193 NC_000962.3 Mycobrowser_v2 CDS 382879 383541 . - 0 Rv0314c Rv0314c Unknown Possible conserved membrane protein Rv0314c, (MTCY63.19c), len: 220 aa. Possible conserved membrane protein, with hydrophobic stretch from residues ~75-100. Similar in C-terminal part to Mycobacterium tuberculosis proteins Rv0679c and Rv0680c. O07241 No cell wall and cell processes ML2517 MMAR_0565 MSMEG_5317 Mb0322c NC_000962.3 Mycobrowser_v2 CDS 219996 221723 . - 0 Rv0189c ilvD Involved in valine and isoleucine biosynthesis (at the fourth step) [catalytic activity: 2,3-dihydroxy-3-methylbutanoate = 3-methyl-2- oxobutanoate + H(2)O]. Probable dihydroxy-acid dehydratase IlvD (dad) Rv0189c, (MTCI28.28c), len: 575 aa. Probable ilvD, dihydroxy-acid dehydratase, similar to many e.g. ILVD_LACLA|Q02139 dihydroxy-acid dehydratase (dad) from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (570 aa), FASTA scores: opt: 1605, E(): 0, (46.0% identity in 561 aa overlap). Also similar to ML2608|MLCL622.06c|O06069|ILVD_MYCLE dihydroxy-acid dehydratase from Mycobacterium leprae (564 aa). Contains PS00886 Dihydroxy-acid and 6-phosphogluconate dehydratases signature 1. Belongs to the ILVD / EDD family. Cofactor: binds 1 4FE-4S cluster (potential). P65154 No intermediary metabolism and respiration ML2608 MMAR_0432 MSMEG_0229 Mb0195c NC_000962.3 Mycobrowser_v2 RBS 221856 221859 . + 0 Rv0190 Rv0190 Function unknown Conserved protein Rv0190, (MTCI28.29), len: 96 aa. Conserved protein, highly similar to several hypothetical proteins e.g. SYCSLRA_35|Q55554|SLL0176 hypothetical 18.9 kDa protein from Synechocystis (167 aa), FASTA scores: opt: 237, E(): 5.8e-16, (39.4% identity in 94 aa overlap). Also highly similar to Z95398|MLCL622_7|O06070 from Mycobacterium leprae (135 aa), FASTA score: (82.6% identity in 92 aa overlap). Also similar to hypothetical proteins from Mycobacterium tuberculosis e.g. Rv0967, Rv0030, Rv1766 (42.5% identity in 80 aa overlap). O07434 No conserved hypotheticals ML2609 MMAR_0433 MSMEG_0230 Mb0196 NC_000962.3 Mycobrowser_v2 CDS 221871 222161 . + 0 Rv0190 Rv0190 Function unknown Conserved protein Rv0190, (MTCI28.29), len: 96 aa. Conserved protein, highly similar to several hypothetical proteins e.g. SYCSLRA_35|Q55554|SLL0176 hypothetical 18.9 kDa protein from Synechocystis (167 aa), FASTA scores: opt: 237, E(): 5.8e-16, (39.4% identity in 94 aa overlap). Also highly similar to Z95398|MLCL622_7|O06070 from Mycobacterium leprae (135 aa), FASTA score: (82.6% identity in 92 aa overlap). Also similar to hypothetical proteins from Mycobacterium tuberculosis e.g. Rv0967, Rv0030, Rv1766 (42.5% identity in 80 aa overlap). O07434 No conserved hypotheticals ML2609 MMAR_0433 MSMEG_0230 Mb0196 NC_000962.3 Mycobrowser_v2 RBS 222281 222284 . + 0 Rv0191 Rv0191 Unknown; possibly involved in transport of drug across the membrane. Probable conserved integral membrane protein Rv0191, (MTCI28.30), len: 413 aa. Probable conserved integral membrane protein, member of major facilitator superfamily (MFS) possibly involved in transport of drug, similar to several hypothetical proteins e.g. YDEA_ECOLI|P31122 hypothetical 42.5 kd protein from Escherichia coli (396 aa), FASTA scores: opt: 475, E(): 4.2e-33, (29.7% identity in 381 aa overlap); and to several chloramphenicol resistance proteins e.g. CMLR_STRLI|P31141 chloramphenicol resistance protein from Streptomyces lividans (392 aa), FASTA scores: opt: 394, E(): 6.7e-12, (28.2% identity in 383 aa overlap). Also similar to SVU09991_1 from Mycobacterium tuberculosis. O07435 No cell wall and cell processes ML2610 MMAR_0434 MSMEG_0232 Mb0197 NC_000962.3 Mycobrowser_v2 CDS 222289 223530 . + 0 Rv0191 Rv0191 Unknown; possibly involved in transport of drug across the membrane. Probable conserved integral membrane protein Rv0191, (MTCI28.30), len: 413 aa. Probable conserved integral membrane protein, member of major facilitator superfamily (MFS) possibly involved in transport of drug, similar to several hypothetical proteins e.g. YDEA_ECOLI|P31122 hypothetical 42.5 kd protein from Escherichia coli (396 aa), FASTA scores: opt: 475, E(): 4.2e-33, (29.7% identity in 381 aa overlap); and to several chloramphenicol resistance proteins e.g. CMLR_STRLI|P31141 chloramphenicol resistance protein from Streptomyces lividans (392 aa), FASTA scores: opt: 394, E(): 6.7e-12, (28.2% identity in 383 aa overlap). Also similar to SVU09991_1 from Mycobacterium tuberculosis. O07435 No cell wall and cell processes ML2610 MMAR_0434 MSMEG_0232 Mb0197 NC_000962.3 Mycobrowser_v2 CDS 1078391 1078687 . + 0 Rv0968 Rv0968 Function unknown Conserved protein Rv0968, (MTCY10D7.06c), len: 98 aa. Conserved protein, part of cso operon, similar to NP_301579.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (92 aa). Also highly similar to conserved hypothetical proteins from Mycobacterium tuberculosis e.g. Rv3269 (93 aa), FASTA score: (51.1% identity in 94 aa overlap); and Rv1993c (90 aa). P64779 No conserved hypotheticals MMAR_1430,MMAR_2537,MMAR_4875 MSMEG_6059 Mb0993 NC_000962.3 Mycobrowser_v2 CDS 223564 224664 . + 0 Rv0192 Rv0192 Function unknown Conserved hypothetical protein Rv0192, (MTCI28.31), len: 366 aa. Conserved hypothetical protein. Has Gly- Arg-rich region followed by highly Pro-rich repetitive region near N-terminus. Similar in C-terminus to other hypothetical proteins e.g. Q49706|B1496_F2_81|U00013 from Mycobacterium leprae (271 aa), FASTA scores: opt: 375, E(): 3.2e-24, (36.1% identity in 255 aa overlap); YV09_MYCTU|Q11149|cY20G9.09 hypothetical 47.9 kDa protein from Mycobacterium tuberculosis (451 aa), FASTA scores: opt: 330, E(): 3.2e-13, (35.1% identity in 271 aa overlap). Also similar to Rv0116c, Rv1433, Rv2518c, Rv0483 from Mycobacterium tuberculosis. O07436 No conserved hypotheticals ML2611 MMAR_0435 MSMEG_0233 Mb0198 NC_000962.3 Mycobrowser_v2 RBS 237194 237197 . + 0 Rv0200 Rv0200 Unknown Possible conserved transmembrane protein Rv0200, (MTV033.08), len: 229 aa. Possible conserved transmembrane protein, equivalent to Z95398|MLCL622.14 from Mycobacterium leprae (229 aa), FASTA scores: opt: 1147, E(): 0, (74.7% identity in 229 aa overlap). Also some similarity to Rv1973 from Mycobacterium tuberculosis (160 aa); and Rv1362c|Z75555|MTCY02B10_26 (220 aa), FASTA scores: opt: 134, E(): 0.063, (25.8% identity in 159 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53651 No cell wall and cell processes ML2615 MMAR_0440 MSMEG_0236 Mb0206 NC_000962.3 Mycobrowser_v2 CDS 237206 237895 . + 0 Rv0200 Rv0200 Unknown Possible conserved transmembrane protein Rv0200, (MTV033.08), len: 229 aa. Possible conserved transmembrane protein, equivalent to Z95398|MLCL622.14 from Mycobacterium leprae (229 aa), FASTA scores: opt: 1147, E(): 0, (74.7% identity in 229 aa overlap). Also some similarity to Rv1973 from Mycobacterium tuberculosis (160 aa); and Rv1362c|Z75555|MTCY02B10_26 (220 aa), FASTA scores: opt: 134, E(): 0.063, (25.8% identity in 159 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53651 No cell wall and cell processes ML2615 MMAR_0440 MSMEG_0236 Mb0206 NC_000962.3 Mycobrowser_v2 RBS 226865 226869 . + 0 Rv0194 Rv0194 Thought to be involved in active transport of drugs across the membrane (export): multidrug resistance by an export mechanism. Responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane. Probable transmembrane multidrug efflux pump Rv0194, (MTV033.02), len: 1194 aa. Probable multidrug efflux pump (See Danilchanka et al., 2008), highly similar to many e.g. U62129|STU62129_2|T30293 ABC transport protein homolog from Salmonella typhi (1218 aa), FASTA scores: opt: 1116, E(): 0, (36.3% identity in 1209 aa overlap); CAB66302.1|AL136519 ABC transporter protein ATP-binding component from Streptomyces coelicolor (1243 aa); I84547 mdl protein from Escherichia coli (1143 aa); etc. Also similar to MTCY50_9 and MTCY50_10 from Mycobacterium tuberculosis, FASTA score: (33.8% identity in 574 aa overlap). Contains two PS00017 ATP/GTP-binding site motif A (P-loop) and one PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). Alternative start possible at 1823 but no RBS. O53645 No cell wall and cell processes MMAR_5223 Mb0200 NC_000962.3 Mycobrowser_v2 CDS 226878 230462 . + 0 Rv0194 Rv0194 Thought to be involved in active transport of drugs across the membrane (export): multidrug resistance by an export mechanism. Responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane. Probable transmembrane multidrug efflux pump Rv0194, (MTV033.02), len: 1194 aa. Probable multidrug efflux pump (See Danilchanka et al., 2008), highly similar to many e.g. U62129|STU62129_2|T30293 ABC transport protein homolog from Salmonella typhi (1218 aa), FASTA scores: opt: 1116, E(): 0, (36.3% identity in 1209 aa overlap); CAB66302.1|AL136519 ABC transporter protein ATP-binding component from Streptomyces coelicolor (1243 aa); I84547 mdl protein from Escherichia coli (1143 aa); etc. Also similar to MTCY50_9 and MTCY50_10 from Mycobacterium tuberculosis, FASTA score: (33.8% identity in 574 aa overlap). Contains two PS00017 ATP/GTP-binding site motif A (P-loop) and one PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). Alternative start possible at 1823 but no RBS. O53645 No cell wall and cell processes MMAR_5223 Mb0200 NC_000962.3 Mycobrowser_v2 CDS 230899 231534 . + 0 Rv0195 Rv0195 Possibly sensor part of a two component regulatory system. Possible two component transcriptional regulatory protein (probably LuxR-family) Rv0195, (MTV033.03), len: 211 aa. Possible two-component response regulator, luxR family, similar to many e.g. U00008|ECOHU49_15 regulatory protein narP from Escherichia coli strain K12 (225 aa), FASTA scores: opt: 232, E(): 7.3e-09, (29.2% identity in 219 aa overlap). Start chosen by similarity. Contains probable helix-turn-helix motif at aa 166-187 (Score 1164, +3.15 SD). O53646 No regulatory proteins Mb0201 NC_000962.3 Mycobrowser_v2 RBS 231638 231641 . + 0 Rv0196 Rv0196 Possibly involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0196, (MTV033.04), len: 194 aa. Possible transcriptional regulatory protein, similar to two Bacillus subtilis regulators: P42105|YXAF_BACSU hypothetical 21.0 kDa protein (191 aa), FASTA scores: opt: 323, E(): 2.1e-15, (30.9% identity in 181 aa overlap); and Z99105|BSUB0002_9 negative regulator of the lincomycin operon (188 aa), FASTA scores: opt: 255, E(): 1e-10, (25.9 identity in 185 aa overlap). O53647 No regulatory proteins MMAR_0436 MSMEG_3520 Mb0202 NC_000962.3 Mycobrowser_v2 CDS 231647 232231 . + 0 Rv0196 Rv0196 Possibly involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0196, (MTV033.04), len: 194 aa. Possible transcriptional regulatory protein, similar to two Bacillus subtilis regulators: P42105|YXAF_BACSU hypothetical 21.0 kDa protein (191 aa), FASTA scores: opt: 323, E(): 2.1e-15, (30.9% identity in 181 aa overlap); and Z99105|BSUB0002_9 negative regulator of the lincomycin operon (188 aa), FASTA scores: opt: 255, E(): 1e-10, (25.9 identity in 185 aa overlap). O53647 No regulatory proteins MMAR_0436 MSMEG_3520 Mb0202 NC_000962.3 Mycobrowser_v2 RBS 232219 232224 . + 0 Rv0197 Rv0197 Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0197, (MTV033.05), len: 762 aa. Possible oxidoreductase, similar to others e.g. 9948789|AAG06102.1|AE004699_7|B83307 probable molybdopterin oxidoreductase from Pseudomonas aeruginosa strain PAO1 (769 aa); 5441785|CAB46809.1|AL096811|T36812 probable dehydrogenase from Streptomyces coelicolor (747 aa), FASTA scores: opt: 617, E(): 9.8e-30, (29.9% identity in 762 aa overlap); BAB04334.1|AP001509 assimilatory nitrate reductase (catalytic subunit) from Bacillus halodurans (743 aa); etc. O53648 No intermediary metabolism and respiration ML2612 MMAR_0437 MSMEG_3521 Mb0203 NC_000962.3 Mycobrowser_v2 CDS 232231 234519 . + 0 Rv0197 Rv0197 Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0197, (MTV033.05), len: 762 aa. Possible oxidoreductase, similar to others e.g. 9948789|AAG06102.1|AE004699_7|B83307 probable molybdopterin oxidoreductase from Pseudomonas aeruginosa strain PAO1 (769 aa); 5441785|CAB46809.1|AL096811|T36812 probable dehydrogenase from Streptomyces coelicolor (747 aa), FASTA scores: opt: 617, E(): 9.8e-30, (29.9% identity in 762 aa overlap); BAB04334.1|AP001509 assimilatory nitrate reductase (catalytic subunit) from Bacillus halodurans (743 aa); etc. O53648 No intermediary metabolism and respiration ML2612 MMAR_0437 MSMEG_3521 Mb0203 NC_000962.3 Mycobrowser_v2 CDS 2204212 2204706 . + 0 Rv1961 Rv1961 Unknown Hypothetical protein Rv1961, MTCY09F9.03c, len: 164 aa. Hypothetical unknown protein. P95253 No conserved hypotheticals Mb1996 NC_000962.3 Mycobrowser_v2 CDS 234516 236507 . - 0 Rv0198c zmp1 Function unknown; hydrolyzes peptides and/or proteins. Probable zinc metalloprotease Zmp1 Rv0198c, (MTV033.06c), len: 663 aa. Probable zmp1, zinc metalloprotease, equivalent to Z95398|MLCL622.12c from Mycobacterium leprae (667 aa), FASTA scores: opt: 3710, E(): 0, (80.8 % identity in 667 aa overlap). Also similar to many other metalloproteases e.g. members of the eukaryotic neprilysin family: P08473|NEP_HUMAN neprilysin (749 aa), FASTA scores: opt: 872, E(): 0, (31.1% identity in 692 aa overlap); Q07744|PEPO_LACLA neutral endopeptidase from Lactococcus lactis (626 aa), FASTA scores: opt: 862, E(): 0, (30.0% identity in 654 aa overlap). Contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. Belongs to peptidase family M13 (zinc metalloprotease); also known as the neprilysin subfamily. O53649 No intermediary metabolism and respiration ML2613 MMAR_0438 MSMEG_0234 Mb0204c NC_000962.3 Mycobrowser_v2 RBS 236515 236519 . - 0 Rv0198c zmp1 Function unknown; hydrolyzes peptides and/or proteins. Probable zinc metalloprotease Zmp1 Rv0198c, (MTV033.06c), len: 663 aa. Probable zmp1, zinc metalloprotease, equivalent to Z95398|MLCL622.12c from Mycobacterium leprae (667 aa), FASTA scores: opt: 3710, E(): 0, (80.8 % identity in 667 aa overlap). Also similar to many other metalloproteases e.g. members of the eukaryotic neprilysin family: P08473|NEP_HUMAN neprilysin (749 aa), FASTA scores: opt: 872, E(): 0, (31.1% identity in 692 aa overlap); Q07744|PEPO_LACLA neutral endopeptidase from Lactococcus lactis (626 aa), FASTA scores: opt: 862, E(): 0, (30.0% identity in 654 aa overlap). Contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. Belongs to peptidase family M13 (zinc metalloprotease); also known as the neprilysin subfamily. O53649 No intermediary metabolism and respiration ML2613 MMAR_0438 MSMEG_0234 Mb0204c NC_000962.3 Mycobrowser_v2 CDS 236550 237209 . + 0 Rv0199 Rv0199 Unknown Probable conserved membrane protein Rv0199, (MTV033.07), len: 219 aa. Probable conserved membrane protein, equivalent to Z95398|MLCL622.13 from Mycobacterium leprae (224 aa), FASTA scores: opt: 920, E(): 0, (67.7% identity in 220 aa overlap). Also some similarity to Mce-associated membrane proteins from Mycobacterium tuberculosis e.g. Rv0178, Rv0175, etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53650 No cell wall and cell processes ML2614 MMAR_0439 MSMEG_0235 Mb0205 NC_000962.3 Mycobrowser_v2 CDS 238392 241292 . - 0 Rv0202c mmpL11 Unknown. Thought to be involved in fatty acid transport. Probable conserved transmembrane transport protein MmpL11 Rv0202c, (MTV033.10c), len: 966 aa. Probable mmpL11, conserved transmembrane transport protein (see citation below), equivalent to Z95398|MLCL622.16c from Mycobacterium leprae (1014 aa), FASTA scores: opt: 4076, E(): 0, (72.8% identity in 1017 aa overlap). Member of RND superfamily, similar to several putative transport proteins e.g. P96687 from Bacillus subtilis (724 aa), FASTA scores: opt: 594, E(): 9.1e-29, (26.9% identity in 717 aa overlap); etc. Belongs to the MmpL family. P65374 No cell wall and cell processes ML2617 MMAR_0442 MSMEG_0241 Mb0208c NC_000962.3 Mycobrowser_v2 RBS 241501 241507 . + 0 Rv0203 Rv0203 Unknown Possible exported protein Rv0203, (MTV033.11), len: 136 aa. Possible exported protein (has hydrophobic stretch near N-terminus). Some similarity to part of U02459|LDU02459_1 hypothetical protein from Leishmania donovani (741 aa), FASTA score: opt: 111, E(): 9.1, (30.0% identity in 90 aa overlap). O53654 No cell wall and cell processes MMAR_0443 MSMEG_0242 Mb0209 NC_000962.3 Mycobrowser_v2 CDS 241514 241924 . + 0 Rv0203 Rv0203 Unknown Possible exported protein Rv0203, (MTV033.11), len: 136 aa. Possible exported protein (has hydrophobic stretch near N-terminus). Some similarity to part of U02459|LDU02459_1 hypothetical protein from Leishmania donovani (741 aa), FASTA score: opt: 111, E(): 9.1, (30.0% identity in 90 aa overlap). O53654 No cell wall and cell processes MMAR_0443 MSMEG_0242 Mb0209 NC_000962.3 Mycobrowser_v2 CDS 241976 243214 . - 0 Rv0204c Rv0204c Unknown Probable conserved transmembrane protein Rv0204c, (MTV033.12c), len: 412 aa. Probable conserved transmembrane protein (see citation below), equivalent, but has C-terminal extension, to Z95398|MLCL622.17c from Mycobacterium leprae (367 aa), FASTA scores: opt: 2002, E(): 0, (82.4% identity in 374 aa overlap). Some similarity to Rv0585c from Mycobacterium tuberculosis. Nucleotide position 242299 in the genome sequence has been corrected, C:G resulting in V306L. O53655 No cell wall and cell processes ML2618 MMAR_0444 MSMEG_0248 Mb0210c NC_000962.3 Mycobrowser_v2 CDS 1003957 1004484 . + 0 Rv0901 Rv0901 Function unknown Possible conserved exported or membrane protein Rv0901, (MTCY31.29), len: 175 aa. Possible conserved exported or membrane protein, with hydrophobic N-terminus at aa 7-25. Shows some similarity in C-terminus to O33070|Z99494|MLCB57.59 hypothetical protein from Mycobacterium leprae (113 aa), FASTA scores: opt: 204, E(): 3.2e-12, (44.9% identity in 78 aa overlap). P64757 No cell wall and cell processes ML2125 MMAR_4635 Mb0925 NC_000962.3 Mycobrowser_v2 CDS 243384 244487 . + 0 Rv0205 Rv0205 Unknown Probable conserved transmembrane protein Rv0205, (MTV033.13), len: 367 aa. Possible conserved transmembrane protein, similar to hypothetical proteins from many bacteria e.g. AL0209|SC4H8_6 from Streptomyces coelicolor (402 aa), FASTA scores: opt: 436, E(): 1.7e-21, (27.2% identity in 349 aa overlap); Z99117|BSUB0014_221 from Bacillus subtilis (353 aa), FASTA scores: opt: 394, E(): 8.6e-19, (28.7% identity in 324 aa overla). O53656 No cell wall and cell processes ML2619 MMAR_0445 MSMEG_0249 Mb0211 NC_000962.3 Mycobrowser_v2 CDS 244484 247318 . - 0 Rv0206c mmpL3 Unknown. Thought to be involved in fatty acid transport. Possible conserved transmembrane transport protein MmpL3 Rv0206c, (MTV033.14c, MTCY08D5.01c), len: 944 aa. Possible mmpL3, conserved transmembrane transport protein (see Tekaia et al., 1999), equivalent to Z95398|MLCL622.18c from Mycobacterium leprae (955 aa), FASTA scores: opt: 806, E(): 1.8e-21, (57.2% identity in 243 aa overlap). Member of RND superfamily, similar to others. Belongs to the MmpL family. O53657 No cell wall and cell processes ML2620 MMAR_0446 MSMEG_0250 Mb0212c NC_000962.3 Mycobrowser_v2 CDS 247384 248112 . - 0 Rv0207c Rv0207c Function unknown Conserved hypothetical protein Rv0207c, (MTCY08D5.02c), len: 242 aa. Conserved hypothetical protein, equivalent to Z95398|MLCL622_19 from Mycobacterium leprae (261 aa), FASTA scores: E(): 0, (60.8 identity in 199 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P96389 No conserved hypotheticals ML2621 MSMEG_0251 Mb0213c NC_000962.3 Mycobrowser_v2 CDS 438302 438739 . - 0 Rv0360c Rv0360c Function unknown Conserved protein Rv0360c, (MTCY13E10.22c), len: 145 aa. Conserved protein, equivalent to AL023514|MLCB4_16|CAA18948.1|AL023514|MLCB4.27c hypothetical protein from Mycobacterium leprae (137 aa), FASTA scores: opt: 793, E(): 0, (85.4% identity in 137 aa overlap). And similar to AL049754|SCH10_25c|T36537 hypothetical protein from Streptomyces coelicolor (143 aa), FASTA scores: opt: 497, E(): 3.2e-27, (55.8% identity in 138 aa overlap). O06310 No conserved hypotheticals ML0284 MMAR_0672 MSMEG_0754 Mb0367c NC_000962.3 Mycobrowser_v2 RBS 438742 438747 . - 0 Rv0360c Rv0360c Function unknown Conserved protein Rv0360c, (MTCY13E10.22c), len: 145 aa. Conserved protein, equivalent to AL023514|MLCB4_16|CAA18948.1|AL023514|MLCB4.27c hypothetical protein from Mycobacterium leprae (137 aa), FASTA scores: opt: 793, E(): 0, (85.4% identity in 137 aa overlap). And similar to AL049754|SCH10_25c|T36537 hypothetical protein from Streptomyces coelicolor (143 aa), FASTA scores: opt: 497, E(): 3.2e-27, (55.8% identity in 138 aa overlap). O06310 No conserved hypotheticals ML0284 MMAR_0672 MSMEG_0754 Mb0367c NC_000962.3 Mycobrowser_v2 CDS 248115 248906 . - 0 Rv0208c Rv0208c Causes methylation Hypothetical methlytransferase (methylase) Rv0208c, (MTCY08D5.03c), len: 263 aa. Hypothetical methyltransferase, equivalent to Z95398|MLCL622_20 from Mycobacterium leprae (279 aa), FASTA score: (64.2% identity in 246 aa overlaps). Also similar to others e.g. 10178368|CAC08407.1|AL392177|Q9F305|MT04_STRCO|SCD17A.03c hypothetical methlytransferase from Streptomyces coelicolor (271 aa). Could start at aa 7. P67498 No intermediary metabolism and respiration ML2622 MMAR_0448 MSMEG_0252 Mb0214c NC_000962.3 Mycobrowser_v2 CDS 249038 250123 . + 0 Rv0209 Rv0209 Unknown Hypothetical protein Rv0209, (MTCY08D5.04), len: 361 aa. Hypothetical unknown protein. P96391 No conserved hypotheticals MMAR_0449 MSMEG_0253 Mb0215 NC_000962.3 Mycobrowser_v2 CDS 251782 253602 . + 0 Rv0211 pckA Rate-limiting gluconeogenic enzyme [catalytic activity: GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2]. Probable iron-regulated phosphoenolpyruvate carboxykinase [GTP] PckA (phosphoenolpyruvate carboxylase) (PEPCK)(pep carboxykinase) Rv0211, (MTCY08D5.06), len: 606 aa. Probable pckA (alternate gene names: pckG and pck1), iron-regulated phosphoenolpyruvate carboxykinase [GTP], equivalent to Z95398|MLCL622_21 probable phosphoenolpyruvate carboxykinase from Mycobacterium leprae (609 aa), FASTA score: (86.1% identity in 605 aa overlap). Also highly similar to others e.g. PPCK_NEOFR|P22130 phosphoenolpyruvate carboxykinase [GTP] (608 aa), FASTA scores: opt: 2287, E(): 0, (55.9% identity in 598 aa overlap). Contains PS00505 Phosphoenolpyruvate carboxykinase (GTP) signature. Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. P65686 No intermediary metabolism and respiration ML2624 MMAR_0451 MSMEG_0255 Mb0217 NC_000962.3 Mycobrowser_v2 CDS 387888 388550 . + 0 Rv0320 Rv0320 Unknown Possible conserved exported protein Rv0320, (MTCY63.25), len: 220 aa. Possible conserved exported protein, similar to some hypothetical proteins and to the middle part of a peptidase: NP_066789.1|10657900|AAG21739.1|AF116907 putative peptidase from Rhodococcus equi (546 aa). Also similar to Rv1728c|MTCY04C12.13c from Mycobacterium tuberculosis (256 aa), FASTA scores: opt: 497, E(): 1.2e-26, (41.8% identity in 225 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). O07246 No cell wall and cell processes ML2508 MMAR_0599 MSMEG_0651 Mb0328 NC_000962.3 Mycobrowser_v2 CDS 253669 254640 . - 0 Rv0212c nadR Involved in transcriptional mechanism. Possible transcriptional regulatory protein NadR (probably AsnC-family) Rv0212c, (MTCY08D5.07c), len: 323 aa. Possible nadR (alternate gene name: nadI), transcriptional regulator, similar to others e.g. NADR_ECOLI|P27278 transcriptional regulator from Escherichia coli (410 aa), FASTA scores: opt: 377, E (): 1e-17, (31.1% identity in 347 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). P96394 No regulatory proteins MMAR_2965 Mb0218c NC_000962.3 Mycobrowser_v2 CDS 254637 255950 . - 0 Rv0213c Rv0213c Causes methylation Possible methyltransferase (methylase) Rv0213c, (MTCY08D5.08c), len: 437 aa. Possible methyltransferase, weakly similar to others methyltransferases e.g. AF127374_30|LINA from Streptomyces lavendulae (611 aa), FASTA scores: opt: 400, E(): 8.1e-19, (27.3% identity in 388 aa overlap); Q50258 fortimicin kl1 methyltransferase (553 aa), FASTA scores: opt: 267, E(): 1.2e-13, (29.3% identity in 351 aa overlap). P96395 No intermediary metabolism and respiration MMAR_1693 Mb0219c NC_000962.3 Mycobrowser_v2 CDS 256064 257677 . + 0 Rv0214 fadD4 Function unknown, but involved in lipid degradation. Probable fatty-acid-CoA ligase FadD4 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv0214, (MTCY08D5.09), len: 537 aa. Probable fadD4, fatty-acid-CoA synthetase, similar to many e.g. 4CL_PINTA|P41636 4-coumarate--CoA ligase (537 aa), FASTA scores: opt: 622, E(): 1e-31, (30.0% identity in 514 aa overlap). Also similar to others from Mycobacterium tuberculosis e.g. MTCY6A4.14 FASTA score: (30.7% identity in 501 aa overlap); MTCY493_27, MTCY07A7_11, MTCI28_6. Contains PS00455 putative AMP-binding domain signature. P96396 No lipid metabolism ML2625 MMAR_0452 MSMEG_0257 Mb0220 NC_000962.3 Mycobrowser_v2 CDS 258913 259926 . + 0 Rv0216 Rv0216 Unknown Double hotdog hydratase Rv0216, (MTCY08D5.11), len: 337 aa. Double hotdog R-specific hydratase of unknown function, shows no activity for crotonyl-CoA, equivalent to Z95398|MLCL622_22 from Mycobacterium leprae (339 aa), FASTA scores: E(): 0, (73.7 identity in 338 aa overlap). Shows structural similarity to six others in Mycobacterium tuberculosis (see Castell et al (2005) below). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P96398 No intermediary metabolism and respiration ML2627 MMAR_0456 MSMEG_0261 Mb0222 NC_000962.3 Mycobrowser_v2 CDS 259923 260831 . - 0 Rv0217c lipW Function unknown; lipolytic enzyme probably involved in cellular metabolism. Possible esterase LipW Rv0217c, (MTCY08D5.12c), len: 302 aa. Possible esterase, showing similarity with others e.g. EST_ACICA|P18773 esterase (303 aa), FASTA scores: opt: 320, E(): 3.2e-13, (29.2% identity in 274 aa overlap). P96399 No intermediary metabolism and respiration MSMEG_0280 Mb0223c NC_000962.3 Mycobrowser_v2 CDS 260924 262252 . + 0 Rv0218 Rv0218 Unknown Probable conserved transmembrane protein Rv0218, (MTCY08D5.13), len: 442 aa. Probable conserved transmembrane protein, some similarity with sulfite oxidases e.g. SUOX_HUMAN|P51687 sulfite oxidase precursor (488 aa), FASTA scores: opt: 153, E(): 0.0087, (28.6% identity in 161 aa overlap); and with some nitrate reductases e.g. NIA_FUSOX|P39863 nitrate reductase (NADPH) (905 aa), FASTA scores: opt: 143, E(): 0.06, (29.3% identity in 92 aa overlap). Also similar to BSUB0017_86 from Mycobacterium tuberculosis. P96400 No cell wall and cell processes MMAR_0461 MSMEG_0998 Mb0224 NC_000962.3 Mycobrowser_v2 CDS 262254 262802 . + 0 Rv0219 Rv0219 Unknown Probable conserved transmembrane protein Rv0219, (MTCY08D5.14), len: 182 aa. Probable conserved transmembrane protein, showing similarity with CAB76992.1|AL159178 putative lipoprotein from Streptomyces coelicolor (163 aa). P96401 No cell wall and cell processes MMAR_0462 Mb0225 NC_000962.3 Mycobrowser_v2 CDS 1836387 1836830 . - 0 Rv1632c Rv1632c Unknown Hypothetical protein Rv1632c, (MTCY01B2.24c), len: 147 aa. Hypothetical unknown protein. O06149 No conserved hypotheticals MMAR_2436 MSMEG_3817 Mb1658c NC_000962.3 Mycobrowser_v2 RBS 1836836 1836839 . - 0 Rv1632c Rv1632c Unknown Hypothetical protein Rv1632c, (MTCY01B2.24c), len: 147 aa. Hypothetical unknown protein. O06149 No conserved hypotheticals MMAR_2436 MSMEG_3817 Mb1658c NC_000962.3 Mycobrowser_v2 CDS 262812 264023 . + 0 Rv0220 lipC Function unknown; lipolytic enzyme probably involved in cellular metabolism. Probable esterase LipC Rv0220, (MTCY08D5.15), len: 403 aa. Probable esterase, similar to others proteins and esterases from various organisms and Mycobacterium tuberculosis e.g. Q50681 (431 aa), FASTA scores: opt: 841, E(): 0, (38.2% identity in 408 aa overlap); Rv1426c, Rv1399c, etc. Contains PS00122 Carboxylesterases type-B serine active site. P96402 No intermediary metabolism and respiration MMAR_0463 MSMEG_0289 Mb0226 NC_000962.3 Mycobrowser_v2 CDS 264067 265476 . + 0 Rv0221 Rv0221 May be involved in synthesis of triacylglycerol Possible triacylglycerol synthase (diacylglycerol acyltransferase) Rv0221, (MTCY08D5.16), len: 469 aa. Possible triacylglycerol synthase (See Daniel et al., 2004), similar to other proteins from Mycobacterium tuberculosis e.g. Q50680|Rv2285|MT2343|MTCY339.25c 47.7 kDa protein (445 aa), FASTA scores: opt: 455, E(): 8.1e-23, (26.7% identity in 461 aa overlap); Rv3740c, Rv3734c, etc. P96403 No lipid metabolism MMAR_0464 MSMEG_0290 Mb0227 NC_000962.3 Mycobrowser_v2 CDS 266301 267764 . - 0 Rv0223c Rv0223c Thought to oxidize a wide variety of aliphatic and aromatic aldehydes. Probable aldehyde dehydrogenase Rv0223c, (MTCY08D5.18), len: 487 aa. Probable aldehyde dehydrogenase, similar to others e.g. A75608|6460525|AAF12231.1|AE001862_57 aldehyde dehydrogenase from Deinococcus radiodurans strain R1 (495 aa); Q47943 L-sorbosone dehydrogenase NAD(P) dependent from Gluconobacter oxydans (498 aa), FASTA scores: opt: 1157, E (): 0, (42.1% identity in 482 aa overlap); etc. Also similar to Rv0768, Rv2858c, etc from Mycobacterium tuberculosis. Contains PS00687 Aldehyde dehydrogenases glutamic acid active site; and PS00070 Aldehyde dehydrogenases cysteine active site. Belongs to the aldehyde dehydrogenases family. P96405 No intermediary metabolism and respiration ML2585 MMAR_0472 MSMEG_0309 Mb0228c NC_000962.3 Mycobrowser_v2 CDS 267863 268627 . - 0 Rv0224c Rv0224c Causes methylation Possible methyltransferase (methylase) Rv0224c, (MTCY08D5.19c), len: 254 aa. Possible methyltransferase, showing weak similarity with other methyltransferases e.g. P74388 sterol-C-methyltransferase (318 aa), FASTA scores: opt: 190, E(): 3.6e-05, (33.3% identity in 114 aa overlap). Equivalent to AL022486|MLCB1883_1 from Mycobacterium leprae (269 aa), FASTA scores: opt: 1456, E(): 0, (82.9% identity in 252 aa overlap). Also some similarity with MTCY21B4.22c from Mycobacterium tuberculosis FASTA score: (30.1% identity in 136 aa overlap). P96406 No intermediary metabolism and respiration ML2584 MMAR_0473 Mb0229c NC_000962.3 Mycobrowser_v2 CDS 268663 269817 . + 0 Rv0225 Rv0225 Possibly involved in LPS biosynthesis. Possible conserved protein Rv0225, (MTCY08D5.20), len: 384 aa. Possible conserved protein involved in LPS biosynthesis, similar to O26275 LPS biosynthesis RFBU related protein (382 aa), FASTA scores: opt: 426, E(): 1.2e-20, (28.2% identity in 394 aa overlap). Some similarity with Rv3032 from Mycobacterium tuberculosis FASTA score: (31.6% identity in 228 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). P96407 No cell wall and cell processes ML2583 MMAR_0474 MSMEG_0311 Mb0230 NC_000962.3 Mycobrowser_v2 RBS 296797 296802 . + 0 Rv0246 Rv0246 Unknown Probable conserved integral membrane protein Rv0246, (MTV034.12), len: 436 aa (start uncertain). Probable conserved integral membrane protein, similar to Rv2209|1237062|CAA94252.1|Z70283|Q10398|YM09_MYCTU from Mycobacterium tuberculosis (512 aa), FASTA scores: opt: 712, E(): 0, (33.2% identity in 422 aa overlap). O53668 No cell wall and cell processes MMAR_0508 Mb0252 NC_000962.3 Mycobrowser_v2 CDS 296809 298119 . + 0 Rv0246 Rv0246 Unknown Probable conserved integral membrane protein Rv0246, (MTV034.12), len: 436 aa (start uncertain). Probable conserved integral membrane protein, similar to Rv2209|1237062|CAA94252.1|Z70283|Q10398|YM09_MYCTU from Mycobacterium tuberculosis (512 aa), FASTA scores: opt: 712, E(): 0, (33.2% identity in 422 aa overlap). O53668 No cell wall and cell processes MMAR_0508 Mb0252 NC_000962.3 Mycobrowser_v2 CDS 269834 271564 . - 0 Rv0226c Rv0226c Unknown Probable conserved transmembrane protein Rv0226c, (MTCY08D5.21c), len: 576 aa. Probable conserved transmembrane protein, equivalent, except in N-terminal part, to AC32114.1|AL583926 conserved membrane protein from Mycobacterium leprae (600 aa), FASTA scores: opt: 2086, E(): 0, (70.3% identity in 579 aa overlap). Also similar to AL021411|SC7H1_20 from Streptomyces coelicolor (483 aa), FASTA scores: opt: 180, E(): 0.00028, (26.5 identity in 388 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P96408 No cell wall and cell processes ML2582 MMAR_0475 MSMEG_0315 Mb0231c NC_000962.3 Mycobrowser_v2 CDS 271574 272839 . - 0 Rv0227c Rv0227c Unknown Probable conserved membrane protein Rv0227c, (MTCY08D5.22c), len: 421 aa. Possible conserved membrane protein, equivalent to AL022486|MLCB1883_4 from Mycobacterium leprae (448 aa), FASTA scores: opt: 2148, E(): 0, (76.6% identity in 423 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P96409 No cell wall and cell processes ML2581 MMAR_0476 MSMEG_0317 Mb0232c NC_000962.3 Mycobrowser_v2 CDS 273055 274278 . + 0 Rv0228 Rv0228 Function unknown; probably involved in cellular metabolism. Probable integral membrane acyltransferase Rv0228, (MTCY08D5.23), len: 407 aa. Probable integral membrane acyltransferase, equivalent to 3063875|CAA18555.1|AL022486|T44870 acyltransferase from Mycobacterium leprae (384 aa), FASTA scores: opt: 2004, E(): 0, (79.3% identity in 381 aa overlap). Also similar to others e.g. Q11064 probable acyltransferase CY50.28C (383 aa), FASTA scores: opt: 372, E(): 2.6e-16, (35.9% identity in 359 aa overlap); Q00718|MDMB_STRMY acyltransferase. Very similar to Rv0111, Rv1254, etc from Mycobacterium tuberculosis. P96410 No intermediary metabolism and respiration ML2580 MMAR_0477 MSMEG_0319 Mb0233 NC_000962.3 Mycobrowser_v2 CDS 274306 274986 . - 0 Rv0229c Rv0229c Unknown Possible conserved membrane protein with PIN domain Rv0229c, (MTCY08D5.24c), len: 226 aa. Possible conserved membrane protein with PIN domain in C-terminal half, similar to several others from Mycobacterium tuberculosis. Has some similarity with Rv2757c|D70880 from Mycobacterium tuberculosis (138 aa). (See Arcus et al., 2005). FASTA scores: E(): 1e-15, (45.3% identity in 137 aa overlap), and Rv0301, Rv2546, etc. Also some similarity with Q48177 virulence associated protein C (132 aa), FASTA scores: opt: 101, E(): 0.6, (24.3% identity in 136 aa overlap). Contains PS00626 Regulator of chromosome condensation (RCC1) signature 2. P96411 No cell wall and cell processes Mb0234c NC_000962.3 Mycobrowser_v2 CDS 479789 480160 . + 0 Rv0401 Rv0401 Unknown Probable conserved transmembrane protein Rv0401, (MTCY04D9.14), len: 123 aa. Probable conserved transmembrane protein, equivalent to AL023514|MLCB4_9 putative integral membrane protein from Mycobacterium leprae (122 aa), FASTA scores: opt: 548, E(): 4.4e-32, (66.9% identity in 121 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P95210 No cell wall and cell processes ML0271 MMAR_0699 MSMEG_0245 Mb0407 NC_000962.3 Mycobrowser_v2 CDS 274983 275963 . - 0 Rv0230c php Enzymatic activity unknown [catalytic activity: aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol]. Probable phosphotriesterase Php (parathion hydrolase) (PTE) (aryldialkylphosphatase) (paraoxonase) (a-esterase) (aryltriphosphatase) (paraoxon hydrolase) Rv0230c, (MTCY08D5.26c), len: 326 aa. Probable php, phosphotriesterase, similar to others e.g. AAK42653.1|AE006849 putative aryldialkylphosphatase (phosphotriesterase) (paraoxonase) from Sulfolobus solfataricus (314 aa); PHP_ECOLI|P45548 phosphotriesterase homology protein from Escherichia coli (292 aa), FASTA scores: opt: 408, E(): 7.1e-20, (31.1% identity in 305 aa overlap); OPD_FLASP|P16648 parathion hydrolase precursor (365 aa), FASTA scores: opt: 319, E(): 5.1e-14, (34.5% identity in 333 aa overlap); etc. Belongs to the phosphotriesterase family. Cofactor: contains 2 moles of zinc per subunit. P96413 No lipid metabolism ML2579 MMAR_0487 MSMEG_0320 Mb0235c NC_000962.3 Mycobrowser_v2 CDS 289104 289337 . + 0 Rv0239 vapB24 Unknown Possible antitoxin VapB24 Rv0239, (MTV034.05), len: 77 aa. Possible vapB24, antitoxin, part of toxin-antitoxin (TA) operon with Rv0240. Weakly similar to others e.g. Rv1839c|Z83859|MTCY359_34 from Mycobacterium tuberculosis (87 aa). See Arcus et al. 2005. FASTA scores: opt: 88, E(): 5, (40.0% identity in 45 aa overlap). O53662 No virulence, detoxification, adaptation Mb0245 NC_000962.3 Mycobrowser_v2 CDS 276058 277764 . + 0 Rv0231 fadE4 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE4 Rv0231, (MTCY08D5.27), len: 568 aa. Probable fadE4, acyl-CoA dehydrogenase, similar to many e.g. O29752 acyl-CoA dehydrogenase (ACD-3) from Archaeoglobus fulgidus (576 aa), FASTA scores: opt: 1788, E(): 0, (51.0% identity in 577 aa overlap); ACDB_BACSU|P45857 acyl-CoA dehydrogenase from Bacillus subtilis (379 aa), FASTA scores: opt: 232, E(): 2.2e- 08, (21.6% identity in 291 aa overlap). P96414 No lipid metabolism ML2578 MMAR_0488 Mb0236 NC_000962.3 Mycobrowser_v2 CDS 277899 278588 . + 0 Rv0232 Rv0232 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably TetR/AcrR-family) Rv0232, (MTCY08D5.28), len: 229 aa. Probable transcriptional regulatory protein, TetR/AcrR family, similar to others e.g. YIXD_BACSU|P32398 hypothetical transcriptional regulator (191 aa), FASTA scores: opt: 149, E(): 0.0014, (21.5% identity in 158 aa overlap). Also similar to MTV030_11 from Mycobacterium tuberculosis. Contains PS01081 Bacterial regulatory proteins, TetR family signature, and probable helix-turn helix motif from aa 33-54 (Score 1142, +3.08 SD). Belongs to the TetR/AcrR family of transcriptional regulators. P96415 No regulatory proteins ML2575 MMAR_0489 Mb0237 NC_000962.3 Mycobrowser_v2 CDS 278585 279529 . + 0 Rv0233 nrdB Involved in the DNA replication pathway (first reaction). Provides the precursors necessary for DNA synthesis [catalytic activity: 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O = ribonucleoside diphosphate + reduced thioredoxin]. Ribonucleoside-diphosphate reductase (beta chain) NrdB (ribonucleotide reductase small chain) Rv0233, (MTCY08D5.29), len: 314 aa. nrdB (alternate gene name: rnrS) ribonucleoside-diphosphate reductase, beta chain, similar to others e.g. RIR2_SCHPO|P36603 ribonucleoside-diphosphate reductase (391 aa), FASTA scores: opt: 168, E(): 0.00018, (26.1% identity in 199 aa overlap); etc. Belongs to the ribonucleoside diphosphate reductase small chain family. Cofactor: iron, manganese P96416 No information pathways ML2574 MSMEG_0358 Mb0238 NC_000962.3 Mycobrowser_v2 RBS 305442 305448 . + 0 Rv0253 nirD Involved in nitrate assimilation (denitrification); required for activity of the reductase [catalytic activity: 3 NAD(P)H + nitrite = 3 NAD(P)+ + NH4OH + H2O.] Probable nitrite reductase [NAD(P)H] small subunit NirD Rv0253, (MTV034.19), len: 118 aa. Probable nirD, nitrite reductase [NAD(P)H] small subunit, similar to others e.g. P23675|NIRD_ECOLI|B3366|Z4727|ECS4217 from Escherichia coli strains K12 and O157:H7 (108 aa), FASTA scores: opt: 271, E():1.7e-12, (41.9% identity in 105 aa overlap). Associates with NIRB|Rv0252. O53675 No intermediary metabolism and respiration ML2554 MMAR_0517 MSMEG_0428 Mb0259 NC_000962.3 Mycobrowser_v2 CDS 305453 305809 . + 0 Rv0253 nirD Involved in nitrate assimilation (denitrification); required for activity of the reductase [catalytic activity: 3 NAD(P)H + nitrite = 3 NAD(P)+ + NH4OH + H2O.] Probable nitrite reductase [NAD(P)H] small subunit NirD Rv0253, (MTV034.19), len: 118 aa. Probable nirD, nitrite reductase [NAD(P)H] small subunit, similar to others e.g. P23675|NIRD_ECOLI|B3366|Z4727|ECS4217 from Escherichia coli strains K12 and O157:H7 (108 aa), FASTA scores: opt: 271, E():1.7e-12, (41.9% identity in 105 aa overlap). Associates with NIRB|Rv0252. O53675 No intermediary metabolism and respiration ML2554 MMAR_0517 MSMEG_0428 Mb0259 NC_000962.3 Mycobrowser_v2 CDS 279605 281140 . - 0 Rv0234c gabD1 Involved in 4-aminobutyrate (GABA) degradation pathway [catalytic activity: succinate semialdehyde + NAD(P)(+) + H(2)O = succinate + NAD(P)H]. Succinate-semialdehyde dehydrogenase [NADP+] dependent (SSDH) GabD1 Rv0234c, (MTCY08D5.30c), len: 511 aa. gabD1, succinate-semialdehyde dehydrogenase [NADP+] dependent, equivalent to AL022486|MLCB1883_6 probable aldehyde dehydrogenase from Mycobacterium leprae (457 aa), FASTA scores: opt: 2617, E(): 0, (85.7% identity in 455 aa overlap). Also highly similar to Q55585|GABD|SLR0370 probable succinate-semialdehyde dehydrogenase from Synechocystis sp. strain PCC 6803 (454 aa), FASTA scores: opt: 1676, E(): 0, (55.8% identity in 455 aa overlap); and similar to others e.g. GABD_ECOLI|P25526 succinate-semialdehyde dehydrogenase from Escherichia coli (482 aa), FASTA scores: opt: 929, E(): 0, (36.5% identity in 452 aa overlap); etc. Note that similar to other cytosolic aldehyde dehydrogenases with EC number: 1.2.1.3. Also similar to Rv0768|aldA semialdehyde dehydrogenase from Mycobacterium tuberculosis (489 aa); and gabD2|Rv1731|MTCY04C12.16 possible succinate-semialdehyde dehydrogenase [NADP+] dependent from Mycobacterium tuberculosis (518 aa). Contains PS00070 aldehyde dehydrogenases cysteine active site. Belongs to the aldehyde dehydrogenases family. Could start at different site by homology. Note that previously known as gabD2. P71989 No intermediary metabolism and respiration ML2573 MMAR_0490 Mb0239c NC_000962.3 Mycobrowser_v2 CDS 281166 282614 . - 0 Rv0235c Rv0235c Unknown Probable conserved transmembrane protein Rv0235c, (MTCY08D5.31c), len: 482 aa. Probable conserved transmembrane protein, highly similar to AL133278|CAB61913.1|SCM11_2 putative integral membrane protein from Streptomyces coelicolor (470 aa), FASTA scores: opt: 2116, E(): 0, (61.8% identity in 474 aa overlap); and similar to hypothetical proteins from other organisms e.g. Q13392|384D8_7 hypothetical protein (579 aa), FASTA scores: opt: 355, E(): 6.9e-17, (28.5% identity in 569 aa overlap). P96418 No cell wall and cell processes ML2572 MMAR_0493 MSMEG_1761 Mb0240c NC_000962.3 Mycobrowser_v2 CDS 282649 286851 . - 0 Rv0236c aftD Involved in the biosynthesis of the mycobacterial cell wall arabinan Possible arabinofuranosyltransferase AftD Rv0236c, (MTV034.01c, MTV034.02c, MTCY08D5.32c), len: 1400 aa. Possible aftD, arabinofuranosyltransferase (See Skovierova et al., 2009). Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). Probable conserved transmembrane protein, equivalent to AL022486|CAC32102.1|MLCB1883_7 possible integral membrane protein from Mycobacterium leprae (1440 aa), FASTA scores: opt: 7491, E(): 0, (78.8% identity in 1397 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P96419 No cell wall and cell processes ML2570 MMAR_0496 MSMEG_0359 Mb0241c NC_000962.3 Mycobrowser_v2 CDS 286898 287071 . - 0 Rv0236A Rv0236A Unknown Small secreted protein Rv0236A, len: 57 aa. Small secreted protein. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P0A5C5 No cell wall and cell processes ML2569A MMAR_0497 MSMEG_0360 Mb0242c NC_000962.3 Mycobrowser_v2 CDS 287186 288352 . + 0 Rv0237 lpqI Unknown Probable conserved lipoprotein LpqI Rv0237, (MTV034.03), len: 388 aa. Probable lpQI, conserved lipoprotein, equivalent to AL022486|MLCB1883_8|T44873 probable secreted hydrolase from Mycobacterium leprae (387 aa), FASTA scores: opt: 1831, E(): 0, (73.3% identity in 390 aa overlap). Also similar to other lipoproteins and various hydrolases e.g. P40406|2126897|YBBD_BACSU|I39839 hypothetical 70.6 KDA lipoprotein from Bacillus subtilis (642 aa); P48823|HEXA_ALTSO beta-hexosaminidase a precursor from alteromonas SP. (598 aa), FASTA scores: opt: 415, E(): 5.8e-17, (31.2% identity in 343 aa overlap); PCC6803|P74340 beta-glucosidase from Synechocystis sp. (538 aa), FASTA scores: opt: 414, E(): 6.1e-17, (30.6 identity in 320 aa overlap). Contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Q7DA74 No cell wall and cell processes ML2569 MMAR_0499 MSMEG_0361 Mb0243 NC_000962.3 Mycobrowser_v2 CDS 310774 311517 . - 0 Rv0259c Rv0259c Function unknown Conserved hypothetical protein Rv0259c, (MTCY06A4.03c), len: 247 aa. Conserved hypothetical protein, showing some similarity to Rv2393|Z81368|MTCY253_28 from Mycobacterium tuberculosis (281 aa), FASTA scores: E(): 9.5e-16, (33.6 % identity in 235 aa overlap). Also some similarity with CAC33938.1|AL589708 putative secreted protein from Streptomyces coelicolor (248 aa). P95216 No conserved hypotheticals MMAR_0519 MSMEG_0431 Mb0265c NC_000962.3 Mycobrowser_v2 RBS 288416 288421 . + 0 Rv0238 Rv0238 Possibly involved in transcriptional mechanism. Possible transcriptional regulatory protein (probably TetR-family) Rv0238, (MTV034.04), len: 204 aa. Possible transcriptional regulatory protein, TetR family, equivalent to AL022486|MLCB1883_9|T44874 probable transcription regulator from Mycobacterium leprae (208 aa), FASTA scores: opt: 1029, E(): 0, (80.9% identity in 199 aa overlap). Also similar to others e.g. CAB77290.1|AL160312 putative TetR-family regulatory protein from Streptomyces coelicolor (240 aa). Also similar to Mycobacterium tuberculosis proteins Z95120|Rv3208 (228 aa), FASTA scores: opt: 266, E(): 8.3e-12, (28.1% identity in 196 aa overlap); and Rv1019 (197 aa). O53661 No regulatory proteins ML2568 MMAR_0500 MSMEG_0363 Mb0244 NC_000962.3 Mycobrowser_v2 CDS 288428 289042 . + 0 Rv0238 Rv0238 Possibly involved in transcriptional mechanism. Possible transcriptional regulatory protein (probably TetR-family) Rv0238, (MTV034.04), len: 204 aa. Possible transcriptional regulatory protein, TetR family, equivalent to AL022486|MLCB1883_9|T44874 probable transcription regulator from Mycobacterium leprae (208 aa), FASTA scores: opt: 1029, E(): 0, (80.9% identity in 199 aa overlap). Also similar to others e.g. CAB77290.1|AL160312 putative TetR-family regulatory protein from Streptomyces coelicolor (240 aa). Also similar to Mycobacterium tuberculosis proteins Z95120|Rv3208 (228 aa), FASTA scores: opt: 266, E(): 8.3e-12, (28.1% identity in 196 aa overlap); and Rv1019 (197 aa). O53661 No regulatory proteins ML2568 MMAR_0500 MSMEG_0363 Mb0244 NC_000962.3 Mycobrowser_v2 CDS 289812 290654 . - 0 Rv0241c htdX Involved in fatty acid synthesis type II (fas-II) Probable 3-hydroxyacyl-thioester dehydratase HtdX Rv0241c, (MTV034.07c), len: 280 aa. Probable htdX, 3-hydroxyacyl-thioester dehydratase (See Gurvitz et al., 2009), highly similar to MLCB1883.17c|T44876063881|CAA18566.1|AL022486 hypothetical protein from Mycobacterium leprae (280 aa), FASTA scores: opt: 1564, E(): 0, (81.8% identity in 280 aa overlap); and CAC32097.1|AL583926 conserved hypothetical protein from Mycobacterium leprae (300 aa). Shows structural similarity to six others in Mycobacterium tuberculosis (see Castell et al (2005) below). Also similar to proteins from other organisms e.g. CAB77291.1|AL160312 putative dehydratase from Streptomyces coelicolor (291 aa); part of BAA92930.1|AB032743 fatty acid synthetase beta subunit from Pichia angusta (2060 aa). Predicted to be an outer membrane protein (See Song et al., 2008). O53664 No intermediary metabolism and respiration ML2566 MMAR_0502 MSMEG_0371 Mb0247c NC_000962.3 Mycobrowser_v2 RBS 290663 290666 . - 0 Rv0241c htdX Involved in fatty acid synthesis type II (fas-II) Probable 3-hydroxyacyl-thioester dehydratase HtdX Rv0241c, (MTV034.07c), len: 280 aa. Probable htdX, 3-hydroxyacyl-thioester dehydratase (See Gurvitz et al., 2009), highly similar to MLCB1883.17c|T44876063881|CAA18566.1|AL022486 hypothetical protein from Mycobacterium leprae (280 aa), FASTA scores: opt: 1564, E(): 0, (81.8% identity in 280 aa overlap); and CAC32097.1|AL583926 conserved hypothetical protein from Mycobacterium leprae (300 aa). Shows structural similarity to six others in Mycobacterium tuberculosis (see Castell et al (2005) below). Also similar to proteins from other organisms e.g. CAB77291.1|AL160312 putative dehydratase from Streptomyces coelicolor (291 aa); part of BAA92930.1|AB032743 fatty acid synthetase beta subunit from Pichia angusta (2060 aa). Predicted to be an outer membrane protein (See Song et al., 2008). O53664 No intermediary metabolism and respiration ML2566 MMAR_0502 MSMEG_0371 Mb0247c NC_000962.3 Mycobrowser_v2 CDS 302866 305427 . + 0 Rv0252 nirB Involved in nitrate assimilation (denitrification) (at the second step). This enzyme is a FAD flavoprotein that also contains a siroheme and one 2FE-2S iron-sulfur center [catalytic activity: 3 NAD(P)H + nitrite = 3 NAD(P)+ + NH4OH + H2O.] Probable nitrite reductase [NAD(P)H] large subunit [FAD flavoprotein] NirB Rv0252, (MTV034.18), len: 853 aa. Probable nirB (alternate gene name: nasB), nitrite reductase [NAD(P)H] large subunit, flavoprotein containing siroheme and a 2FE-2S iron-sulfur centre. Highly similar to many others bacterial enzymes e.g. P08201|NIRB_ECOLI nitrite reductase (NAD(P)H) large subunit from Escherichia coli strain K12 (847 aa), FASTA scores: opt: 2775, E(): 0, (49.8% identity in 840 aa overlap); Q06458|NIRB_KLEPN nitrite reductase (NAD(P)H) large subunit (957 aa), FASTA scores: opt: 2902, E(): 0, (54.2% identity in 827 aa overlap). Contains PS00365 Nitrite and sulfite reductases iron-sulfur/siroheme-binding site. Homodimer which associates with NIRD|Rv0253. Cofactors: FAD; Iron; Siroheme. O53674 No intermediary metabolism and respiration ML2555 MMAR_0516 MSMEG_0427 Mb0258 NC_000962.3 Mycobrowser_v2 CDS 2544289 2544621 . - 0 Rv2269c Rv2269c Unknown Hypothetical protein Rv2269c, (MTCY339.41), len: 110 aa. Unknown protein; questionable ORF. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P64965 No conserved hypotheticals Mb2292c NC_000962.3 Mycobrowser_v2 CDS 290665 292029 . - 0 Rv0242c fabG4 Involved in the fatty acid biosynthesis pathway (first reduction step) [catalytic activity: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH]. Probable 3-oxoacyl-[acyl-carrier protein] reductase FabG4 (3-ketoacyl-acyl carrier protein reductase) Rv0242c, (MTV034.08c), len: 454 aa. Probable fabG4, 3-oxoacyl-[acyl-carrier protein] reductase, equivalent to 3063883|CAA18568.1|AL022486|MLCB1883_13|T44878 3-oxoacyl-[acyl-carrier protein] reductase homolog from Mycobacterium leprae (454 aa), FASTA scores: opt: 2486, E(): 0, (84.8% identity in 454 aa overlap). C-terminal part highly similar to many FabG proteins e.g. U39441|VHU3944 1_2 from Vibrio harveyi (244 aa), FASTA scores: opt: 562, E(): 3.4e-28, (40.2% identity in 241 aa overlap); U91631|PAU91631_3 from Pseudomonas aeruginosa (247 aa), FASTA scores: opt: 584, E(): 1.5e-29, (44.4% identity in 241 aa overlap). Has N-terminal extension of ~200 aa and C-terminal part contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. O53665 No lipid metabolism ML2565 MMAR_0503 MSMEG_0372 Mb0248c NC_000962.3 Mycobrowser_v2 RBS 292038 292041 . - 0 Rv0242c fabG4 Involved in the fatty acid biosynthesis pathway (first reduction step) [catalytic activity: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH]. Probable 3-oxoacyl-[acyl-carrier protein] reductase FabG4 (3-ketoacyl-acyl carrier protein reductase) Rv0242c, (MTV034.08c), len: 454 aa. Probable fabG4, 3-oxoacyl-[acyl-carrier protein] reductase, equivalent to 3063883|CAA18568.1|AL022486|MLCB1883_13|T44878 3-oxoacyl-[acyl-carrier protein] reductase homolog from Mycobacterium leprae (454 aa), FASTA scores: opt: 2486, E(): 0, (84.8% identity in 454 aa overlap). C-terminal part highly similar to many FabG proteins e.g. U39441|VHU3944 1_2 from Vibrio harveyi (244 aa), FASTA scores: opt: 562, E(): 3.4e-28, (40.2% identity in 241 aa overlap); U91631|PAU91631_3 from Pseudomonas aeruginosa (247 aa), FASTA scores: opt: 584, E(): 1.5e-29, (44.4% identity in 241 aa overlap). Has N-terminal extension of ~200 aa and C-terminal part contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. O53665 No lipid metabolism ML2565 MMAR_0503 MSMEG_0372 Mb0248c NC_000962.3 Mycobrowser_v2 RBS 292161 292164 . + 0 Rv0243 fadA2 Function unknown, but involved in lipid degradation [catalytic activity: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA]. Probable acetyl-CoA acyltransferase FadA2 (3-ketoacyl-CoA thiolase) (beta-ketothiolase) Rv0243, (MTV034.09), len: 440 aa. Probable fadA2, acetyl-CoA acyltransferase (3-acyl-CoA thiolase), equivalent, but shorter 17 aa, to AL022486|MLCB1883_14T44879 acetyltransferase from Mycobacterium leprae (457 aa), FASTA scores: opt: 250 7, E(): 0, (87.6% identity in 435 aa overlap). Also highly similar to many e.g. G83046|PA478 probable acyl-CoA thiolase from Pseudomonas aeruginosa (425 aa); AB77293.1|AL160312 putative ketoacyl CoA thiolase from Streptomyces coelicolor (428 aa); P76503|7449731|YFCY_ECOLI|D65007|B2342 probable 3-ketoacyl-CoA thiolase (acetyl-CoA acyltransferase) (beta-ketothiolase) from Escherichia coli strain K-12 (436 aa), FASTA scores: opt: 914, E(): 0, (38.2% identity in 434 aa overlap); P55084|ECHB_HUMAN mitochondrial trifunctonal enzyme (474 aa), FASTA scores: opt: 881, E(): 0, (37.7 identity in 451 aa overlap). Contains PS00099 Thiolases active site. Belongs to the thiolase family. O86361 No lipid metabolism ML2564 MMAR_0504 MSMEG_0373 Mb0249 NC_000962.3 Mycobrowser_v2 CDS 292171 293493 . + 0 Rv0243 fadA2 Function unknown, but involved in lipid degradation [catalytic activity: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA]. Probable acetyl-CoA acyltransferase FadA2 (3-ketoacyl-CoA thiolase) (beta-ketothiolase) Rv0243, (MTV034.09), len: 440 aa. Probable fadA2, acetyl-CoA acyltransferase (3-acyl-CoA thiolase), equivalent, but shorter 17 aa, to AL022486|MLCB1883_14T44879 acetyltransferase from Mycobacterium leprae (457 aa), FASTA scores: opt: 250 7, E(): 0, (87.6% identity in 435 aa overlap). Also highly similar to many e.g. G83046|PA478 probable acyl-CoA thiolase from Pseudomonas aeruginosa (425 aa); AB77293.1|AL160312 putative ketoacyl CoA thiolase from Streptomyces coelicolor (428 aa); P76503|7449731|YFCY_ECOLI|D65007|B2342 probable 3-ketoacyl-CoA thiolase (acetyl-CoA acyltransferase) (beta-ketothiolase) from Escherichia coli strain K-12 (436 aa), FASTA scores: opt: 914, E(): 0, (38.2% identity in 434 aa overlap); P55084|ECHB_HUMAN mitochondrial trifunctonal enzyme (474 aa), FASTA scores: opt: 881, E(): 0, (37.7 identity in 451 aa overlap). Contains PS00099 Thiolases active site. Belongs to the thiolase family. O86361 No lipid metabolism ML2564 MMAR_0504 MSMEG_0373 Mb0249 NC_000962.3 Mycobrowser_v2 CDS 293798 295633 . - 0 Rv0244c fadE5 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE5 Rv0244c, (MTV034.10c), len: 611 aa. Probable fadE5, acyl-CoA dehydrogenase, equivalent to AL022486|MLCB1883_15 from Mycobacterium leprae (611 aa), FASTA scores: opt: 3598, E(): 0, (89.4% identity in 611 aa overlap). Also highly similar to AL0211|MTV007.14 from Mycobacterium tuberculosis (609 aa), FASTA scores: opt: 2576, E(): 0, (64.6% identity in 611 aa overlap); and to various other bacterial proteins described as putative acyl-CoA dehydrogenases e.g. AE0010|AE001025_6 from Archaeoglobus fulgidus (387 aa), FASTA scores: opt: 229, E(): 6.8e-08, (29.8% identity in 409 aa overlap); etc. O53666 No lipid metabolism ML2563 MMAR_0505 MSMEG_0406 Mb0250c NC_000962.3 Mycobrowser_v2 RBS 295640 295646 . - 0 Rv0244c fadE5 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE5 Rv0244c, (MTV034.10c), len: 611 aa. Probable fadE5, acyl-CoA dehydrogenase, equivalent to AL022486|MLCB1883_15 from Mycobacterium leprae (611 aa), FASTA scores: opt: 3598, E(): 0, (89.4% identity in 611 aa overlap). Also highly similar to AL0211|MTV007.14 from Mycobacterium tuberculosis (609 aa), FASTA scores: opt: 2576, E(): 0, (64.6% identity in 611 aa overlap); and to various other bacterial proteins described as putative acyl-CoA dehydrogenases e.g. AE0010|AE001025_6 from Archaeoglobus fulgidus (387 aa), FASTA scores: opt: 229, E(): 6.8e-08, (29.8% identity in 409 aa overlap); etc. O53666 No lipid metabolism ML2563 MMAR_0505 MSMEG_0406 Mb0250c NC_000962.3 Mycobrowser_v2 CDS 296005 296493 . + 0 Rv0245 Rv0245 Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0245, (MTV034.11), len: 162 aa. Possible oxidoreductase, equivalent to AL022486|MLCB1883_17|T44882 probable oxidoreductase from Mycobacterium leprae (162 aa), FASTA scores: opt: 860, E(): 0, (83.4% identity in 157 aa overlap). Also similar to several hypothetical proteins and various oxidoreductases e.g. AAK24246.1|AE005898 NADH:riboflavin 5'-phosphate oxidoreductase from Caulobacter crescentus (174 aa); Q02058|DIM6_STRCO|CAA45048.1 actinorhodin polyketide dimerase from streptomyces coelicolor (177 aa), FASTA scores: opt: 308, E(): 3. 2e-15, (37.8% identity in 143 aa overlap). Also similar to Z84498|Rv1939|MTCY09F9.25c from Mycobacterium tuberculosis (171 aa), FASTA scores: opt: 517, E(): 3.5e-30, (49.4% identity in 158 aa overlap). O53667 No intermediary metabolism and respiration ML2561 MMAR_0506 MSMEG_0415 Mb0251 NC_000962.3 Mycobrowser_v2 RBS 1282300 1282304 . + 0 Rv1156 Rv1156 Function unknown Conserved protein Rv1156, (MTCI65.23), len: 195 aa. Conserved protein, highly similar to CAC32318.1|AL583944 conserved hypothetical protein from Streptomyces coelicolor (197 aa). O06554 No conserved hypotheticals ML1507 MMAR_4296 MSMEG_5156 Mb1187 NC_000962.3 Mycobrowser_v2 CDS 1282306 1282893 . + 0 Rv1156 Rv1156 Function unknown Conserved protein Rv1156, (MTCI65.23), len: 195 aa. Conserved protein, highly similar to CAC32318.1|AL583944 conserved hypothetical protein from Streptomyces coelicolor (197 aa). O06554 No conserved hypotheticals ML1507 MMAR_4296 MSMEG_5156 Mb1187 NC_000962.3 Mycobrowser_v2 CDS 298116 298862 . - 0 Rv0247c Rv0247c Involved in interconversion of fumarate and succinate (aerobic respiration) [catalytic activity: succinate + acceptor = fumarate + reduced acceptor]. Probable succinate dehydrogenase [iron-sulfur subunit] (succinic dehydrogenase) Rv0247c, (MTV034.13c), len: 248 aa. Probable succinate dehydrogenase, iron-sulfur subunit, highly similar to CAC44313.1|AL596043 putative succinate dehydrogenase iron-sulfur subunit from Streptomyces coelicolor (259 aa); and similar to iron-sulphur protein subunits of fumarate reductase or succinate dehydrogenases from many bacteria e.g. NP_147618.1|7521083|B72691 fumarate reductase iron-sulfur protein from Aeropyrum pernix (305 aa); NP_069516.1|2649932|AAB90556.1|AE001057 succinate dehydrogenase iron-sulfur subunit B (sdhB) from Archaeoglobus fulgidus (236 aa); etc. Also similar to Q10761|FRDB_MYCTU|7431693|F70762 fumarate reductase iron-sulfur protein from Mycobacterium tuberculosis (247 aa), FASTA scores: opt: 358, E():1e-16, (31.3% identity in 214 aa overlap). Contains PS00197 2Fe-2S ferredoxins, iron-sulfur binding region signature. Note that succinate dehydrogenase forms generally part of an enzyme complex containing four subunits: a flavoprotein (Rv0248c ?), an iron-sulfur (Rv0247c ?), and two hydrophobic anchor proteins (Rv0249c ?). O53669 No intermediary metabolism and respiration ML2560 MMAR_0510 MSMEG_0417 Mb0253c NC_000962.3 Mycobrowser_v2 RBS 298867 298870 . - 0 Rv0247c Rv0247c Involved in interconversion of fumarate and succinate (aerobic respiration) [catalytic activity: succinate + acceptor = fumarate + reduced acceptor]. Probable succinate dehydrogenase [iron-sulfur subunit] (succinic dehydrogenase) Rv0247c, (MTV034.13c), len: 248 aa. Probable succinate dehydrogenase, iron-sulfur subunit, highly similar to CAC44313.1|AL596043 putative succinate dehydrogenase iron-sulfur subunit from Streptomyces coelicolor (259 aa); and similar to iron-sulphur protein subunits of fumarate reductase or succinate dehydrogenases from many bacteria e.g. NP_147618.1|7521083|B72691 fumarate reductase iron-sulfur protein from Aeropyrum pernix (305 aa); NP_069516.1|2649932|AAB90556.1|AE001057 succinate dehydrogenase iron-sulfur subunit B (sdhB) from Archaeoglobus fulgidus (236 aa); etc. Also similar to Q10761|FRDB_MYCTU|7431693|F70762 fumarate reductase iron-sulfur protein from Mycobacterium tuberculosis (247 aa), FASTA scores: opt: 358, E():1e-16, (31.3% identity in 214 aa overlap). Contains PS00197 2Fe-2S ferredoxins, iron-sulfur binding region signature. Note that succinate dehydrogenase forms generally part of an enzyme complex containing four subunits: a flavoprotein (Rv0248c ?), an iron-sulfur (Rv0247c ?), and two hydrophobic anchor proteins (Rv0249c ?). O53669 No intermediary metabolism and respiration ML2560 MMAR_0510 MSMEG_0417 Mb0253c NC_000962.3 Mycobrowser_v2 CDS 298863 300803 . - 0 Rv0248c Rv0248c Involved in interconversion of fumarate and succinate (aerobic respiration) [catalytic activity: succinate + acceptor = fumarate + reduced acceptor]. Probable succinate dehydrogenase [iron-sulfur subunit] (succinic dehydrogenase) Rv0248c, (MTV034.14c), len: 646 aa. Probable succinate dehydrogenase, flavoprotein subunit, highly similar to flavoprotein subunit of various succinate dehydrogenases e.g. M88696|RIRSDHA_1 flavoprotein from Rickettsia prowazekii (596 aa), FASTA scores: opt: 651, E(): 0, (34.6 % identity in 598 aa overlap). Also similar to truncated U00022_17 flavoprotein from Mycobacterium leprae (401 aa), FASTA scores: opt: 677, E(): 0, (39.0% identity in 423 aa overlap). Note that succinate dehydrogenase forms generally part of an enzyme complex containing four subunits: a flavoprotein (Rv0248c ?), an iron-sulfur (Rv0247c ?), and two hydrophobic anchor proteins (Rv0249c ?). O53670 No intermediary metabolism and respiration ML2559 MMAR_0511 MSMEG_0418 Mb0254c NC_000962.3 Mycobrowser_v2 RBS 300808 300811 . - 0 Rv0248c Rv0248c Involved in interconversion of fumarate and succinate (aerobic respiration) [catalytic activity: succinate + acceptor = fumarate + reduced acceptor]. Probable succinate dehydrogenase [iron-sulfur subunit] (succinic dehydrogenase) Rv0248c, (MTV034.14c), len: 646 aa. Probable succinate dehydrogenase, flavoprotein subunit, highly similar to flavoprotein subunit of various succinate dehydrogenases e.g. M88696|RIRSDHA_1 flavoprotein from Rickettsia prowazekii (596 aa), FASTA scores: opt: 651, E(): 0, (34.6 % identity in 598 aa overlap). Also similar to truncated U00022_17 flavoprotein from Mycobacterium leprae (401 aa), FASTA scores: opt: 677, E(): 0, (39.0% identity in 423 aa overlap). Note that succinate dehydrogenase forms generally part of an enzyme complex containing four subunits: a flavoprotein (Rv0248c ?), an iron-sulfur (Rv0247c ?), and two hydrophobic anchor proteins (Rv0249c ?). O53670 No intermediary metabolism and respiration ML2559 MMAR_0511 MSMEG_0418 Mb0254c NC_000962.3 Mycobrowser_v2 CDS 300834 301655 . - 0 Rv0249c Rv0249c Could be involved in interconversion of fumarate and succinate (aerobic respiration). This hydrophobic component may be required to anchor the catalytic components of the succinate dehydrogenase complex to the cytoplasmic membrane. Probable succinate dehydrogenase [membrane anchor subunit] (succinic dehydrogenase) Rv0249c, (MTV034.15c), len: 273 aa. Probable succinate dehydrogenase, membrane-anchor subunit for succinate dehydrogenase encoded by Rv0247c and Rv0248c. Highly similar to AC44315.1|AL596043 putative integral membrane protein from Streptomyces coelicolor (278 aa). Note that succinate dehydrogenase forms generally part of an enzyme complex containing four subunits: a flavoprotein (Rv0248c ?), an iron-sulfur (Rv0247c ?), and two hydrophobic anchor proteins (Rv0249c ?). O53671 No intermediary metabolism and respiration ML2558 MMAR_0512 MSMEG_0419 Mb0255c NC_000962.3 Mycobrowser_v2 RBS 301665 301669 . - 0 Rv0249c Rv0249c Could be involved in interconversion of fumarate and succinate (aerobic respiration). This hydrophobic component may be required to anchor the catalytic components of the succinate dehydrogenase complex to the cytoplasmic membrane. Probable succinate dehydrogenase [membrane anchor subunit] (succinic dehydrogenase) Rv0249c, (MTV034.15c), len: 273 aa. Probable succinate dehydrogenase, membrane-anchor subunit for succinate dehydrogenase encoded by Rv0247c and Rv0248c. Highly similar to AC44315.1|AL596043 putative integral membrane protein from Streptomyces coelicolor (278 aa). Note that succinate dehydrogenase forms generally part of an enzyme complex containing four subunits: a flavoprotein (Rv0248c ?), an iron-sulfur (Rv0247c ?), and two hydrophobic anchor proteins (Rv0249c ?). O53671 No intermediary metabolism and respiration ML2558 MMAR_0512 MSMEG_0419 Mb0255c NC_000962.3 Mycobrowser_v2 CDS 301735 302028 . - 0 Rv0250c Rv0250c Function unknown. Possibly down-regulated by HSPR|Rv0353. Conserved protein Rv0250c, (MTV034.16c), len: 97 aa. Conserved protein, equivalent to MLCB1883.27c|T44883|3063888|CAA18576.1|AL022486 hypothetical protein from Mycobacterium leprae (98 aa), FASTA scores: opt: 478, E(): 4.4e-28, (72.6% identity in 95 aa overlap). Also similar to C-terminus of AC44316.1|AL596043|SCBAC31E11.05c hypothetical protein from Streptomyces coelicolor (146 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53672 No conserved hypotheticals ML2557 MMAR_0513 MSMEG_0163,MSMEG_0420 Mb0256c NC_000962.3 Mycobrowser_v2 RBS 302035 302038 . - 0 Rv0250c Rv0250c Function unknown. Possibly down-regulated by HSPR|Rv0353. Conserved protein Rv0250c, (MTV034.16c), len: 97 aa. Conserved protein, equivalent to MLCB1883.27c|T44883|3063888|CAA18576.1|AL022486 hypothetical protein from Mycobacterium leprae (98 aa), FASTA scores: opt: 478, E(): 4.4e-28, (72.6% identity in 95 aa overlap). Also similar to C-terminus of AC44316.1|AL596043|SCBAC31E11.05c hypothetical protein from Streptomyces coelicolor (146 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53672 No conserved hypotheticals ML2557 MMAR_0513 MSMEG_0163,MSMEG_0420 Mb0256c NC_000962.3 Mycobrowser_v2 CDS 302173 302652 . - 0 Rv0251c hsp Thought to be involved in the initiation step of translation at high temperature. Bound to 30S ribosomal subunit. Possibly a molecular chaperone. Seems to be regulated positively by SIGE|Rv1221 and negatively by HSPR|Rv0353. Heat shock protein Hsp (heat-stress-induced ribosome-binding protein A) Rv0251c, (MTV034.17c), len: 159 aa. Hsp (alternate gene name: hsp20, hrpA, acr2), heat-stress-induced ribosome-binding protein A (see citations below). Highly similar to AAD39038.1|AF072875_1|AF072875 putative HSP20 from Mycobacterium smegmatis (145 aa), FASTA scores: opt: 479, E(): 2.3e-24, (59.9% identity in 157 aa overlap); and similar to many bacterial and eukaryotic hsp proteins e.g. P12811|HS2C_CHLRE chloroplast heat shock 22KD protein from chlamydomonas reinhardtii (157 aa), FASTA scores: opt: 184, E(): 1.2e-05, (32.4% identity in 142 aa overlap). Also similar to PCC6803 Spore protein sp21 from Synechocystis sp. (146 aa), FASTA scores: opt: 213, E(): 1.2e-07, (30.3 identity in 145 aa overlap). Also similar to P30223|14KD_MYCTU 14 KDA antigen (16 KDA antigen) 19K major membrane protein (HSP 16.3) from Mycobacterium tuberculosis (144 aa). Belongs to the small heat shock protein (HSP20) family. O53673 No virulence, detoxification, adaptation ML2556 MMAR_0515 MSMEG_0424 Mb0257c NC_000962.3 Mycobrowser_v2 RBS 302659 302663 . - 0 Rv0251c hsp Thought to be involved in the initiation step of translation at high temperature. Bound to 30S ribosomal subunit. Possibly a molecular chaperone. Seems to be regulated positively by SIGE|Rv1221 and negatively by HSPR|Rv0353. Heat shock protein Hsp (heat-stress-induced ribosome-binding protein A) Rv0251c, (MTV034.17c), len: 159 aa. Hsp (alternate gene name: hsp20, hrpA, acr2), heat-stress-induced ribosome-binding protein A (see citations below). Highly similar to AAD39038.1|AF072875_1|AF072875 putative HSP20 from Mycobacterium smegmatis (145 aa), FASTA scores: opt: 479, E(): 2.3e-24, (59.9% identity in 157 aa overlap); and similar to many bacterial and eukaryotic hsp proteins e.g. P12811|HS2C_CHLRE chloroplast heat shock 22KD protein from chlamydomonas reinhardtii (157 aa), FASTA scores: opt: 184, E(): 1.2e-05, (32.4% identity in 142 aa overlap). Also similar to PCC6803 Spore protein sp21 from Synechocystis sp. (146 aa), FASTA scores: opt: 213, E(): 1.2e-07, (30.3 identity in 145 aa overlap). Also similar to P30223|14KD_MYCTU 14 KDA antigen (16 KDA antigen) 19K major membrane protein (HSP 16.3) from Mycobacterium tuberculosis (144 aa). Belongs to the small heat shock protein (HSP20) family. O53673 No virulence, detoxification, adaptation ML2556 MMAR_0515 MSMEG_0424 Mb0257c NC_000962.3 Mycobrowser_v2 CDS 305825 306349 . - 0 Rv0254c cobU Involved in cobalamin biosynthesis. Probable bifunctional cobalamin biosynthesis protein CobU: cobinamide kinase + cobinamide phosphate guanylyltransferase Rv0254c, (MTV034.20), len: 174 aa. Probable cobU, cobalamin biosynthesis protein including a cobinamide kinase and cobinamide phosphate guanylyltransferase. Highly similar to many e.g. Q05599|COBU_SALTY cobinamide kinase / cobinamide phosphate guanylyltransferase from Salmonella typhimurium (181 aa), FASTA scores: opt: 308, E(): 1.1e-14, (38.7% identity in 181 aa overlap); P46886|COBU_ECOLI|B1993|Z3153|ECS2788 Bifunctional cobalamin biosynthesis protein cobU from Escherichia coli strains K12 and O157:H7 (181 aa); part of AL096872|SC5F7_10 from Streptomyces coelicolor (397 aa), FASTA scores: opt: 445, E(): 3.6e-23, (46.0% identity in 176 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). O53676 No intermediary metabolism and respiration ML1826 MMAR_3251 MSMEG_4274 Mb0260c NC_000962.3 Mycobrowser_v2 CDS 306374 307858 . - 0 Rv0255c cobQ1 Involved in cobalamin biosynthesis Probable cobyric acid synthase CobQ1 Rv0255c, (MTV034.21c), len: 494 aa. Probable cobQ1, cobyric acid synthase, similar to many e.g. Z46611|RCBLUGNS_8 cobyric acid synthase from R.capsulatus (483 aa), FASTA scores: opt: 1239, E(): 0, (47.1% identity in 493 aa overlap); P29932|COBQ_PSEDE cobyric acid synthase from Pseudomonas denitrificans (484 aa), FASTA scores: opt: 1168, E():0, (44.9% identity in 490 aa overlap); etc. Belongs to the COBB/COBQ family, COBQ subfamily. Note that previously known as cobQ. P0A532 No intermediary metabolism and respiration ML1827 MMAR_3838 MSMEG_2588 Mb0261c NC_000962.3 Mycobrowser_v2 RBS 307863 307866 . - 0 Rv0255c cobQ1 Involved in cobalamin biosynthesis Probable cobyric acid synthase CobQ1 Rv0255c, (MTV034.21c), len: 494 aa. Probable cobQ1, cobyric acid synthase, similar to many e.g. Z46611|RCBLUGNS_8 cobyric acid synthase from R.capsulatus (483 aa), FASTA scores: opt: 1239, E(): 0, (47.1% identity in 493 aa overlap); P29932|COBQ_PSEDE cobyric acid synthase from Pseudomonas denitrificans (484 aa), FASTA scores: opt: 1168, E():0, (44.9% identity in 490 aa overlap); etc. Belongs to the COBB/COBQ family, COBQ subfamily. Note that previously known as cobQ. P0A532 No intermediary metabolism and respiration ML1827 MMAR_3838 MSMEG_2588 Mb0261c NC_000962.3 Mycobrowser_v2 CDS 307877 309547 . - 0 Rv0256c PPE2 Function unknown PPE family protein PPE2 Rv0256c, (MTV034.22c), len: 556 aa. PPE2, Member of the M. tuberculosis PPE family, similar to many e.g. Rv0280, Rv0286, etc. Equivalent to Z98756|MLCB2492.30 from Mycobacterium leprae (572 aa), FASTA scores: opt: 1837, E(): 0, (62.9% identity in 461 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Q79FZ4 No PE/PPE ML1828 MMAR_2944 Mb0262c NC_000962.3 Mycobrowser_v2 RBS 309691 309694 . + 0 Rv0257 Rv0257 Function unknown Conserved hypothetical protein Rv0257, len: 124 aa. Hypothetical protein, orthologue of ML1828A conserved hypothetical protein from Mycobacterium leprae. Replaced Rv0257c (older annotation). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). Q8VKP3 No conserved hypotheticals ML1828A Mb0263 NC_000962.3 Mycobrowser_v2 CDS 309699 310073 . + 0 Rv0257 Rv0257 Function unknown Conserved hypothetical protein Rv0257, len: 124 aa. Hypothetical protein, orthologue of ML1828A conserved hypothetical protein from Mycobacterium leprae. Replaced Rv0257c (older annotation). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). Q8VKP3 No conserved hypotheticals ML1828A Mb0263 NC_000962.3 Mycobrowser_v2 CDS 310294 310749 . - 0 Rv0258c Rv0258c Function unknown Conserved hypothetical protein Rv0258c, (MTCY06A4.02c), len: 151 aa (alternative start possible). Conserved hypothetical protein, showing some similarity to Rv1685c|MTCI125_6 from Mycobacterium tuberculosis (207 aa), FASTA scores: E(): 9.3e-07, (32.1% identity in 140 aa overlap). Also some similarity with AL049819|SCE7_13|T36295 probable transcription regulator from Streptomyces coelicolor (204 aa), FASTA scores: opt: 158, E(): 0.00052, (27.0% identity in 111 aa overlap). P95215 No conserved hypotheticals ML2553 MMAR_0518 Mb0264c NC_000962.3 Mycobrowser_v2 RBS 1779302 1779306 . + 0 Rv1573 Rv1573 Unknown Probable PhiRv1 phage protein Rv1573, (MTCY336.31c), len: 136 aa. Probable phiRv1 phage protein (see citation below). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06617 No insertion seqs and phages Mb1599 NC_000962.3 Mycobrowser_v2 CDS 1779314 1779724 . + 0 Rv1573 Rv1573 Unknown Probable PhiRv1 phage protein Rv1573, (MTCY336.31c), len: 136 aa. Probable phiRv1 phage protein (see citation below). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06617 No insertion seqs and phages Mb1599 NC_000962.3 Mycobrowser_v2 CDS 311514 312659 . - 0 Rv0260c Rv0260c Could be involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0260c, (MTCY0A4.04c), len: 381 aa. Possible two-component response regulator, highly similar to CAB72204.1|AL138851 putative transcriptional regulator from Streptomyces coelicolor (395 aa); and similar to O34394|D69851|YJJA conserved hypothetical protein from Bacillus subtilis (270 aa), FASTA scores: opt: 312, E(): 7.4e-14, (25.8% identity in 267 aa overlap). Also some similarity to regulatory proteins at C-terminal region e.g. CUTR_STRLI|Q03756 transcriptional regulatory protein (217 aa), FASTA scores: opt: 138, E(): 0.02, (30.6% identity in 111 aa overlap). P95217 No regulatory proteins MMAR_0520 MSMEG_0432 Mb0266c NC_000962.3 Mycobrowser_v2 CDS 321331 322722 . + 0 Rv0267 narU Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate. Responsible for the translocation of the substrate across the membrane. Probable integral membrane nitrite extrusion protein NarU (nitrite facilitator) Rv0267, (MTCY06A4.11), len: 463 aa. Probable narU, nitrite extrusion protein, integral membrane protein possibly member of major facilitator superfamily (MFS), similar to other nitrite extrusion proteins e.g. NARU_ECOLI|P37758 nitrite extrusion protein 2 from Escherichia coli (462 aa), FASTA scores: opt: 630, E(): 4.4e-33, (38.9% identity in 463 aa overlap); and NARK_ECOLI|P10903|B1223 nitrite extrusion protein 1 from Escherichia coli strain K12 (463 aa), FASTA scores: opt: 607, E(): 1.3e-31, (42.0% identity in 457 aa overlap). Also similar to Rv0261c, Rv2329c, Rv1737c, and to MLCB22_25 from Mycobacterium leprae (517 aa), FASTA score: (35.1 identity in 459 aa overlap). Belongs to the nark/NASA family of transporters. P95224 No cell wall and cell processes MMAR_0526 Mb0273 NC_000962.3 Mycobrowser_v2 RBS 351837 351841 . + 0 Rv0288 esxH Function unknown. May be involved in virulence. Low molecular weight protein antigen 7 EsxH (10 kDa antigen) (CFP-7) (protein TB10.4) Rv0288, (MT0301, MTV035.16), len: 96 aa. EsxH, low molecular weight protein antigen 7 (10 kDa antigen) (CFP-7) (Protein TB10.4) (see citations below), ala-rich protein; member of mycobacterial protein family containing ESAT-6, very similar to MTV012_33 from Mycobacterium tuberculosis (96 aa), FASTA scores: opt: 566, E(): 0, (84.4% identity in 96 aa overlap). Alternative start codon possible position 351878 (see Rosenkrands et al., 2000). Belongs to the ESAT6 family (see Skjot et al., 2000; 2002; Gey Van Pittius et al., 2001). Note that previously known as cfp7 (alternate gene name: TB10.4). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted possible vaccine candidate (See Zvi et al., 2008). P0A568 No cell wall and cell processes ML2531 MMAR_0547,MMAR_3000 MSMEG_0621 Mb0296 NC_000962.3 Mycobrowser_v2 CDS 351848 352138 . + 0 Rv0288 esxH Function unknown. May be involved in virulence. Low molecular weight protein antigen 7 EsxH (10 kDa antigen) (CFP-7) (protein TB10.4) Rv0288, (MT0301, MTV035.16), len: 96 aa. EsxH, low molecular weight protein antigen 7 (10 kDa antigen) (CFP-7) (Protein TB10.4) (see citations below), ala-rich protein; member of mycobacterial protein family containing ESAT-6, very similar to MTV012_33 from Mycobacterium tuberculosis (96 aa), FASTA scores: opt: 566, E(): 0, (84.4% identity in 96 aa overlap). Alternative start codon possible position 351878 (see Rosenkrands et al., 2000). Belongs to the ESAT6 family (see Skjot et al., 2000; 2002; Gey Van Pittius et al., 2001). Note that previously known as cfp7 (alternate gene name: TB10.4). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted possible vaccine candidate (See Zvi et al., 2008). P0A568 No cell wall and cell processes ML2531 MMAR_0547,MMAR_3000 MSMEG_0621 Mb0296 NC_000962.3 Mycobrowser_v2 CDS 312759 314168 . - 0 Rv0261c narK3 Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate. Responsible for the translocation of the substrate across the membrane. Probable integral membrane nitrite extrusion protein NarK3 (nitrite facilitator) Rv0261c, (MTCY06A4.05c), len: 469 aa. Probable nirK3, nitrite extrusion protein, integral membrane protein possibly member of major facilitator superfamily (MFS), equivalent to AAB41700.1|U72744 nitrite extrusion protein from Mycobacterium fortuitum (471 aa); and 2342627|CAB11406.1|Z98741|T44908 nitrite extrusion protein homolog from Mycobacterium leprae (517 aa; longer in N-terminus). Also similar to other nitrite extrusion proteins e.g. NARK_ECOLI|P10903|B1223 nitrite extrusion protein 1 from Escherichia coli strain K12 (463 aa), FASTA scores: opt: 755, E(): 0, (35.0% identity in 466 aa overlap). Belongs to the nark/NASA family of transporters. P95218 No cell wall and cell processes ML2552 MMAR_0521 MSMEG_0433 Mb0267c NC_000962.3 Mycobrowser_v2 CDS 314309 314854 . - 0 Rv0262c aac Confers resistance to aminoglycosides (gentamicin, tobramycin, dibekacin, netilmicin, and 6'-N-ethylnetilmicin). Aminoglycoside 2'-N-acetyltransferase Aac (Aac(2')-IC) Rv0262c, (MTCY06A4.06c), len: 181 aa. Aac, aminoglycoside 2'-N-acetyltransferase (aac(2')-IC) (see citation below), highly similar to NP_302635.1|NC_002677 aminoglycoside 2'-N-acetyltransferase from Mycobacterium leprae (182 aa); Q49157|AAC2_MYCFO|AAC aminoglycoside 2'-N-acetyltransferase from Mycobacterium fortuitum (195 aa), Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. FASTA scores: opt: 884, E(): 0, (69.1% identity in 181 aa overlap); and P94968|AAC2_MYCSM|AAC aminoglycoside 2'-N-acetyltransferase from Mycobacterium smegmatis (210 aa) (see also citation below). Also similar to Q52424|AAC2_PROST aminoglycoside 2'-N-acetyltransferase from Providencia stuartii (178 aa). Belongs to the AAC(2')-I family of acetyltransferases. Note that previously known as aac(2')-IC. P0A5N0 No virulence, detoxification, adaptation ML2551 MMAR_0522 MSMEG_0434 Mb0268c NC_000962.3 Mycobrowser_v2 CDS 1961291 1961788 . - 0 Rv1735c Rv1735c Function unknown Hypothetical membrane protein Rv1735c, (MTCY04C12.20c), len: 165 aa. Hypothetical membrane protein, similar to part of O58614|PH0884|AP000004 Hypothetical malic acid transport protein from Pyrococcus horikoshii (330 aa), FASTA scores: opt: 167, E(): 0.0003, (29.2% identity in 120 aa overlap). P71993 No cell wall and cell processes Mb1764c NC_000962.3 Mycobrowser_v2 CDS 322764 323273 . - 0 Rv0268c Rv0268c Unknown Hypothetical protein Rv0268c, (MTCY06A4.12c), len: 169 aa. Hypothetical unknown protein. P95225 No conserved hypotheticals Mb0274c NC_000962.3 Mycobrowser_v2 CDS 3195545 3196432 . - 0 Rv2886c Rv2886c Prevents the cointegration of foreign DNA before integration into the chromosome. Probable resolvase Rv2886c, (MTCY274.17c), len: 295 aa. Probable resolvase for IS1539. Contains PS00213 Lipocalin signature. P65045 No insertion seqs and phages Mb2910c NC_000962.3 Mycobrowser_v2 CDS 314864 315766 . - 0 Rv0263c Rv0263c Function unknown Conserved hypothetical protein Rv0263c, (MTCY06A4.07c), len: 300 aa. Conserved hypothetical protein, equivalent to NP_302634.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (305 aa). Also similar to others e.g. AL121596|SC51A_21 hypothetical protein from Streptomyces coelicolor (285 aa), FASTA scores: opt: 714, E(): 0, (45.3% identity in 289 aa overlap); NP_233164.1|NC_002506 conserved hypothetical protein from Vibrio cholerae (309 aa); NP_406216.1|NC_003143 conserved hypothetical protein from Yersinia pestis (316 aa); YH30_HAEIN|P44298|hi1730 hypothetical protein from Haemophilus influenzae (309 aa), FASTA scores: opt: 430, E(): 3e-20, (29.6% identity in 284 aa overlap); etc. Also similar to carboxylases eg NP_415240.1|NC_000913|P75745|YBGK_ECOLI putative carboxylase from Escherichia coli strain K12 (310 aa), FASTA score: (34.6% identity in 286 aa overlap); NP_459698.1|NC_003197 putative carboxylase from Salmonella typhimurium (310 aa); and to middle part of NP_420636.1|NC_002696 urea amidolyase-related protein from Caulobacter crescentus (1207 aa). P95220 No conserved hypotheticals ML2550 MMAR_0523 MSMEG_0435 Mb0269c NC_000962.3 Mycobrowser_v2 CDS 315783 316415 . - 0 Rv0264c Rv0264c Function unknown Conserved hypothetical protein Rv0264c, (MTCY06A4.08c), len: 210 aa. Conserved hypothetical protein, equivalent to CAC32080.1|AL583926 conserved hypothetical protein from Mycobacterium leprae (222 aa). Also similar to others hypothetical proteins e.g. AL121596|SC51A_20 from Streptomyces coelicolor (252 aa), FASTA scores: opt: 420, E(): 2.7e-20, (41.7% identity in 204 aa overlap); P75744|YBGJ_ECOLI hypothetical 23.9 KD protein from Escherichia coli (218 aa), FASTA scores: E(): 2.1e-14, (35.7% identity in 182 aa overlap); YH31_HAEIN|P44299|hi173 hypothetical protein from Haemophilus influenzae (213 aa), FASTA scores: opt: 252, E(): 8.3e-10, (31.1% identity in 183 aa overlap). P95221 No conserved hypotheticals ML2549 MSMEG_0436 Mb0270c NC_000962.3 Mycobrowser_v2 CDS 316511 317503 . - 0 Rv0265c Rv0265c Thought to be involved in iron transport across the membrane (import). Probable periplasmic iron-transport lipoprotein Rv0265c, (MTCY06A4.09c), len: 330 aa. Probable iron-transport lipoprotein, most similar to T36412|5763945|CAB53324.1|AL109974 probable iron-siderophore binding lipoprotein from Streptomyces coelicolor (350 aa); and (N-terminus may be incorrect) to T14166|3560508|AAC82551.1|AF027770 fxuD protein from Mycobacterium smegmatis (420 aa), FASTA scores: opt: 385, E(): 1.5e-16, (32.3% identity in 232 aa overlap). Also similar to AAB97475.1|U02617 DtxR/iron regulated lipoprotein precursor from Corynebacterium diphtheriae (355 aa); FECB_ECOLI|P15028 iron(III) dicitrate-binding periplasmic protein (300 aa), FASTA scores: opt: 191, E(): 2.3e-05, (26.5% identity in 196 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Note that previously known as fecB2. Q7DA51 No cell wall and cell processes ML2548 MMAR_0525 MSMEG_0438 Mb0271c NC_000962.3 Mycobrowser_v2 CDS 317525 321154 . - 0 Rv0266c oplA Catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate [catalytic activity: ATP + 5-oxo-L-proline + 2 H2O = ADP + phosphate + L-glutamate]. Probable 5-oxoprolinase OplA (5-oxo-L-prolinase) (pyroglutamase) (5-OPASE) Rv0266c, (MTCY06A4.10c), len: 1209 aa. Probable oplA, 5-oxoprolinase, highly similar to others or to hypothetical proteins e.g. AAK24340.1|AE005906 hydantoinase/oxoprolinase from Caulobacter crescentus (1196 aa); NP_103129.1|14022305|BAB48915.1|AP002997 5-oxoprolinase from Mesorhizobium loti (1210 aa); CAC48426.1|AL603642 conserved hypothetical protein from Sinorhizobium meliloti (1205 aa); S77037|slr0697|1006579|BAA10729.1|D6400 hypothetical protein from Synechocystis sp. strain PCC 6803 (1252 aa), FASTA scores: opt: 2016, E(): 0, (51.4% identity in 1247 aa overlap); P97608|OPLA_RAT|T42756|11278797 5-oxoprolinase (5-oxo-L-prolinase) (pyroglutamase) (5-OPASE) from Rattus norvegicus (1288 aa); etc. Belongs to the oxoprolinase family. P95223 No intermediary metabolism and respiration Mb0272c NC_000962.3 Mycobrowser_v2 CDS 392039 392263 . + 0 Rv0325 Rv0325 Unknown Hypothetical protein Rv0325, (MTCY63.30), len: 74 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07250 No unknown Mb0333 NC_000962.3 Mycobrowser_v2 RBS 2123668 2123671 . + 0 Rv1874 Rv1874 Unknown Unknown protein Rv1874, (MTCY180.44c), len: 228 aa. Unknown protein. O07755 No conserved hypotheticals ML2040 MMAR_2757 MSMEG_3569 Mb1905 NC_000962.3 Mycobrowser_v2 CDS 2123684 2124370 . + 0 Rv1874 Rv1874 Unknown Unknown protein Rv1874, (MTCY180.44c), len: 228 aa. Unknown protein. O07755 No conserved hypotheticals ML2040 MMAR_2757 MSMEG_3569 Mb1905 NC_000962.3 Mycobrowser_v2 CDS 323338 324531 . - 0 Rv0269c Rv0269c Function unknown Conserved hypothetical protein Rv0269c, (MTCY06A4.13c), len: 397 aa. Conserved hypothetical protein, highly similar to AL079355|SC4C6_19 hypothetical protein from Streptomyces coelicolor (341 aa), FASTA scores: opt: 1019, E(): 0, (46.5% identity in 344 aa overlap), and similar to other proteins e.g. CAC49016.1|AL603644 putative ATP-dependent DNA ligase protein from Sinorhizobium meliloti (636 aa); O34398 YKOU protein from Bacillus subtilis (611 aa), FASTA score: (27.2% identity in 283 aa overlap). Also similar to proteins from Mycobacterium tuberculosis e.g. Rv3062, Rv3731 (both DNA ligases), and Rv0938, Rv3730c. P95226 No conserved hypotheticals ML2547 MMAR_0527 MSMEG_0597 Mb0275c NC_000962.3 Mycobrowser_v2 CDS 324567 326249 . + 0 Rv0270 fadD2 Function unknown, but involved in lipid degradation. Probable fatty-acid-CoA ligase FadD2 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv0270, (MTCY06A4.14), len: 560 aa. Probable fadD2, fatty-acid-CoA synthetase, similar to many e.g. LCFA_ECOLI|P29212 long-chain-fatty-acid--CoA ligase from Escherichia coli (561 aa), FASTA scores: opt: 544, E(): 2.9e-26, (27.7% identity in 535 aa overlap). Also similar to others from Mycobacterium tuberculosis e.g. MTCY493_2, MTCY8D5_9, MTCY6G11_8, etc. Contains PS00455 Putative AMP-binding domain signature. P95227 No lipid metabolism ML2546 MMAR_0528 MSMEG_0599 Mb0276 NC_000962.3 Mycobrowser_v2 CDS 326266 328461 . - 0 Rv0271c fadE6 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE6 Rv0271c, (MTCY06A4.15c), len: 731 aa. Probable fadE6, acyl-CoA dehydrogenase, with C-terminal half similar to many e.g. ACDS_HUMAN|P16219 acyl-CoA dehydrogenase (short-chain) from Homo sapiens (412 aa), FASTA scores: opt: 339, E(): 1.3e-13, (28.1% identity in 288 aa overlap). P95228 No lipid metabolism ML2545 MMAR_0530 MSMEG_0603 Mb0277c NC_000962.3 Mycobrowser_v2 CDS 328575 329708 . - 0 Rv0272c Rv0272c Unknown Unknown protein Rv0272c, (MTCY06A4.16c), len: 377 aa. Unknown protein. P95229 No conserved hypotheticals ML2544 MMAR_0532 MSMEG_0605 Mb0278c NC_000962.3 Mycobrowser_v2 RBS 330411 330414 . + 0 Rv0274 Rv0274 Function unknown Conserved protein Rv0274, (MTV035.02), len: 193 aa. Conserved protein, highly similar to AAK25058.1|AE005973 conserved hypothetical protein from Caulobacter crescentus (174 aa). Shows also some similarity to others hypothetical proteins e.g. AJ002571|BSAJ2571_7 from Bacillus subtilis (316 aa), FASTA scores: opt: 138, E(): 0.033, (27.1% identity in 133 aa overlap). Previous hits with Q56415|M85195 fosfomycin-resistance protein from serratia marcescens (141 aa), FASTA scores: opt: 82, E(): 1.1e -08, (29.1% identity in 151 aa overlap). Contains PS00082 Extradiol ring-cleavage dioxygenases signature near C-terminus. May belong to the vicinal-oxygen-chelate (VOC) superfamily of metalloenzymes (See Rawat et al., 2003). O53680 No conserved hypotheticals ML2542 MSMEG_0608 Mb0280 NC_000962.3 Mycobrowser_v2 CDS 330422 331003 . + 0 Rv0274 Rv0274 Function unknown Conserved protein Rv0274, (MTV035.02), len: 193 aa. Conserved protein, highly similar to AAK25058.1|AE005973 conserved hypothetical protein from Caulobacter crescentus (174 aa). Shows also some similarity to others hypothetical proteins e.g. AJ002571|BSAJ2571_7 from Bacillus subtilis (316 aa), FASTA scores: opt: 138, E(): 0.033, (27.1% identity in 133 aa overlap). Previous hits with Q56415|M85195 fosfomycin-resistance protein from serratia marcescens (141 aa), FASTA scores: opt: 82, E(): 1.1e -08, (29.1% identity in 151 aa overlap). Contains PS00082 Extradiol ring-cleavage dioxygenases signature near C-terminus. May belong to the vicinal-oxygen-chelate (VOC) superfamily of metalloenzymes (See Rawat et al., 2003). O53680 No conserved hypotheticals ML2542 MSMEG_0608 Mb0280 NC_000962.3 Mycobrowser_v2 CDS 330933 331658 . - 0 Rv0275c Rv0275c Could be involved in transcriptional mechanism. Possible transcriptional regulatory protein (possibly TetR-family) Rv0275c, (MTV035.03c), len: 241 aa. Possible transcriptional regulator, TetR family, similar to others e.g. Q9RJE7|SCF81.04c putative TetR-family transcriptional regulator from Streptomyces coelicolor (219 aa); Q9FBI8|SCP8.33c putative TetR-family transcriptional regulator from Streptomyces coelicolor (213 aa); Q9I2Q9|PA1836 probable transcriptional regulator from Pseudomonas aeruginosa (193 aa); etc. Also shows some similarity with Rv0825c from Mycobacterium tuberculosis (213 aa), FASTA scores: opt: 230, E(): 2.7e-07, (32.6% identity in 190 aa overlap). Seems to belong to the TetR/AcrR family of transcriptional regulators (M. tuberculosis regulatory protein family with many TetR orthologues). Q7DA42 No regulatory proteins ML2541 MMAR_0536 MSMEG_0612 Mb0281c NC_000962.3 Mycobrowser_v2 RBS 331736 331740 . + 0 Rv0276 Rv0276 Function unknown Conserved hypothetical protein Rv0276, (MTV035.04), len: 306 aa. Conserved hypothetical protein, similar to Rv2237|Z70692|MTCY427.18 from Mycobacterium tuberculosis (296 aa), FASTA scores: opt: 874, E(): 0, (49.6% identity in 282 aa overlap). O53682 No conserved hypotheticals ML2540 MMAR_0537 MSMEG_0613 Mb0282 NC_000962.3 Mycobrowser_v2 CDS 331748 332668 . + 0 Rv0276 Rv0276 Function unknown Conserved hypothetical protein Rv0276, (MTV035.04), len: 306 aa. Conserved hypothetical protein, similar to Rv2237|Z70692|MTCY427.18 from Mycobacterium tuberculosis (296 aa), FASTA scores: opt: 874, E(): 0, (49.6% identity in 282 aa overlap). O53682 No conserved hypotheticals ML2540 MMAR_0537 MSMEG_0613 Mb0282 NC_000962.3 Mycobrowser_v2 CDS 332708 333136 . - 0 Rv0277c vapC25 Unknown Possible toxin VapC25. Contains PIN domain. Rv0277c, (MTV035.05c), len: 142 aa. Possible vapC25, toxin, part of toxin-antitoxin (TA) operon with Rv0277A, contains PIN domain, see Arcus et al. 2005. Highly similar to others e.g. Rv0749|H70824|2911023|CAA17516.1|AL021958 conserved hypothetical protein from Mycobacterium tuberculosis (142 aa); and Rv2530c, etc. O53683 No virulence, detoxification, adaptation Mb0283c NC_000962.3 Mycobrowser_v2 CDS 333437 336310 . - 0 Rv0278c PE_PGRS3 Function unknown PE-PGRS family protein PE_PGRS3 Rv0278c, (MTV035.06c), len: 957 aa. PE_PGRS3, Member of the Mycobacterium tuberculosis PE family (see citation below), PGRS subfamily of gly-rich proteins, similar to many e.g. Z95890|MTCY28_25|Rv1759c from Mycobacterium tuberculosis (914 aa), FASTA scores: opt: 3849, E(): 0, (67.8% identity in 903 aa overlap). Contains PS00583 pfkB family of carbohydrate kinases signature 1. P56877 No PE/PPE Mb0284c,Mb0285c,Mb0286c NC_000962.3 Mycobrowser_v2 RBS 336317 336321 . - 0 Rv0278c PE_PGRS3 Function unknown PE-PGRS family protein PE_PGRS3 Rv0278c, (MTV035.06c), len: 957 aa. PE_PGRS3, Member of the Mycobacterium tuberculosis PE family (see citation below), PGRS subfamily of gly-rich proteins, similar to many e.g. Z95890|MTCY28_25|Rv1759c from Mycobacterium tuberculosis (914 aa), FASTA scores: opt: 3849, E(): 0, (67.8% identity in 903 aa overlap). Contains PS00583 pfkB family of carbohydrate kinases signature 1. P56877 No PE/PPE Mb0284c,Mb0285c,Mb0286c NC_000962.3 Mycobrowser_v2 CDS 336560 339073 . - 0 Rv0279c PE_PGRS4 Function unknown PE-PGRS family protein PE_PGRS4 Rv0279c, (MTV035.07c), len: 837 aa. PE_PGRS4, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), similar to many e.g. Z95890|MTCY28_25|Rv0278c from Mycobacterium tuberculosis (914 aa), FASTA scores: opt: 2677, E(): 0, (64.5% identity in 926 aa overlap). Q79FY9 No PE/PPE Mb0287c NC_000962.3 Mycobrowser_v2 RBS 339080 339084 . - 0 Rv0279c PE_PGRS4 Function unknown PE-PGRS family protein PE_PGRS4 Rv0279c, (MTV035.07c), len: 837 aa. PE_PGRS4, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), similar to many e.g. Z95890|MTCY28_25|Rv0278c from Mycobacterium tuberculosis (914 aa), FASTA scores: opt: 2677, E(): 0, (64.5% identity in 926 aa overlap). Q79FY9 No PE/PPE Mb0287c NC_000962.3 Mycobrowser_v2 CDS 339364 340974 . + 0 Rv0280 PPE3 Function unknown PPE family protein PPE3 Rv0280, (MTV035.08), len: 536 aa. PPE3, Member of the Mycobacterium tuberculosis PPE family, similar to others e.g. Z80108|MTCY21B4_4|Rv0453 from Mycobacterium tuberculosis (539 aa), FASTA scores: opt: 1131, E(): 0, (51.7% identity in 540 aa overlap). Q79FY8 No PE/PPE MMAR_0538 Mb0288 NC_000962.3 Mycobrowser_v2 CDS 340998 341906 . + 0 Rv0281 Rv0281 Possible methyltransferase Possible S-adenosylmethionine-dependent methyltransferase Rv0281, (MTV035.09), len: 302 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), member of Mycobacterium tuberculosis protein family that includes Rv0726c, Rv0731c, Rv3399, Rv1729c, etc. MTCY31_23 (325 aa), FASTA scores: opt: 1386, E(): 0, (69. 1% identity in 301 aa overlap). Contains possible N-terminal signal sequence. O53686 No lipid metabolism ML2539 MMAR_0539 MSMEG_0614 Mb0289 NC_000962.3 Mycobrowser_v2 RBS 342116 342124 . + 0 Rv0282 eccA3 Function unknown ESX conserved component EccA3. ESX-3 type VII secretion system protein. Rv0282, (MTV035.10), len: 631 aa. eccA3, esx conserved component, ESX-3 type VII secretion system protein, similar to Y14967|MLCB628.18c hypothetical protein from Mycobacterium leprae (573 aa), FASTA scores: opt: 916, E(): 0, (38.7% identity in 568 aa overlap). Also similar to Mycobacterium tuberculosis proteins e.g. Z94121|MTY15F10.26 (619 aa), FASTA scores: opt: 743, E(): 0, (29.9% identity in 612 aa overlap). Member of CFXQ, CBXP family - 9 members in Mycobacterium tuberculosis. Contains PS00017 ATP/GTP-binding site motif A (P-loop). O53687 No cell wall and cell processes ML2537c MMAR_0541 MSMEG_0615 Mb0290 NC_000962.3 Mycobrowser_v2 CDS 342130 344025 . + 0 Rv0282 eccA3 Function unknown ESX conserved component EccA3. ESX-3 type VII secretion system protein. Rv0282, (MTV035.10), len: 631 aa. eccA3, esx conserved component, ESX-3 type VII secretion system protein, similar to Y14967|MLCB628.18c hypothetical protein from Mycobacterium leprae (573 aa), FASTA scores: opt: 916, E(): 0, (38.7% identity in 568 aa overlap). Also similar to Mycobacterium tuberculosis proteins e.g. Z94121|MTY15F10.26 (619 aa), FASTA scores: opt: 743, E(): 0, (29.9% identity in 612 aa overlap). Member of CFXQ, CBXP family - 9 members in Mycobacterium tuberculosis. Contains PS00017 ATP/GTP-binding site motif A (P-loop). O53687 No cell wall and cell processes ML2537c MMAR_0541 MSMEG_0615 Mb0290 NC_000962.3 Mycobrowser_v2 CDS 344022 345638 . + 0 Rv0283 eccB3 Unknown ESX conserved component EccB3. ESX-3 type VII secretion system protein. Possible membrane protein. Rv0283, (MTV035.11), len: 538 aa. eccB3, esx conserved component, ESX-3 type VII secretion system protein, possible membrane protein, similar to several hypothetical mycobacterial proteins e.g. Z94121|MTY15F10_16|Rv3895c from Mycobacterium tuberculosis (495 aa), FASTA scores: opt: 698, E(): 0, (37.6% identity in 492 aa overlap); Rv1782; Rv3450c; Rv3869; and Y14967|MLCB628_16|MLCB628.17c from Mycobacterium leprae (481 aa), FASTA scores: opt: 672, E(): 1.5e-31, (37.2% identity in 506 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53688 No cell wall and cell processes ML2536c MMAR_0542 MSMEG_0616 Mb0291 NC_000962.3 Mycobrowser_v2 RBS 345624 345628 . + 0 Rv0284 eccC3 Unknown ESX conserved component EccC3. ESX-3 type VII secretion system protein. Possible membrane protein. Rv0284, (MTV035.12), len: 1330 aa. eccC3, esx conserved component, ESX-3 type VII secretion system protein, possible membrane protein, similar to products of two adjacent Mycobacterium leprae genes, MLCB628.16c (744 aa) and MLCB628.15c (597 aa); and throughout its length to several large Mycobacterium tuberculosis proteins: Rv3447c, Rv3870, Rv1784, etc. Y14967|MLCB628_ 15 (744 aa), FASTA scores: opt: 942, E(): 0, (33.8% identity in 730 aa overlap); Y14967|MLCB628_14 (597 aa), FASTA scores: opt: 613, E(): 3.1e-30, (31.7% identity in 615 aa overlap); Z94121|MTY15F10_17 (1396 aa), FASTA scores: opt: 652, E(): 2.2e-32, (35.4% identity in 1321 aa overlap); Z95389|MTCY77_19 (1236 aa), FASTA scores: opt 652, E(): 2.2e-32, (35.4% identity in 1321 aa overlap). Contains three PS00017 ATP/GTP-binding site motif A (P-loop). O53689 No cell wall and cell processes ML2535c MMAR_0543 MSMEG_0617 Mb0292 NC_000962.3 Mycobrowser_v2 CDS 345635 349627 . + 0 Rv0284 eccC3 Unknown ESX conserved component EccC3. ESX-3 type VII secretion system protein. Possible membrane protein. Rv0284, (MTV035.12), len: 1330 aa. eccC3, esx conserved component, ESX-3 type VII secretion system protein, possible membrane protein, similar to products of two adjacent Mycobacterium leprae genes, MLCB628.16c (744 aa) and MLCB628.15c (597 aa); and throughout its length to several large Mycobacterium tuberculosis proteins: Rv3447c, Rv3870, Rv1784, etc. Y14967|MLCB628_ 15 (744 aa), FASTA scores: opt: 942, E(): 0, (33.8% identity in 730 aa overlap); Y14967|MLCB628_14 (597 aa), FASTA scores: opt: 613, E(): 3.1e-30, (31.7% identity in 615 aa overlap); Z94121|MTY15F10_17 (1396 aa), FASTA scores: opt: 652, E(): 2.2e-32, (35.4% identity in 1321 aa overlap); Z95389|MTCY77_19 (1236 aa), FASTA scores: opt 652, E(): 2.2e-32, (35.4% identity in 1321 aa overlap). Contains three PS00017 ATP/GTP-binding site motif A (P-loop). O53689 No cell wall and cell processes ML2535c MMAR_0543 MSMEG_0617 Mb0292 NC_000962.3 Mycobrowser_v2 CDS 364044 364469 . + 0 Rv0301 vapC2 Unknown Possible toxin VapC2 Rv0301, (MTCY63.06), len: 141 aa. Possible vapC2, toxin, part of toxin-antitoxin (TA) operon with Rv0300, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. Rv2757c, Rv0229c, Rv2546, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07228 No virulence, detoxification, adaptation Mb0309 NC_000962.3 Mycobrowser_v2 RBS 349614 349617 . + 0 Rv0285 PE5 Function unknown PE family protein PE5 Rv0285, (MTV035.13), len: 102 aa. PE5, Member of the Mycobacterium tuberculosis PE family (see Brennan & Delogu 2002), similar to others e.g. AL0212|MTV012_37 from Mycobacterium tuberculosis (105 aa), FASTA scores: opt: 497, E(): 2.6e-24, (80.4% identity in 102 aa overlap); Z80108|MTCY21B4.03 from Mycobacterium tuberculosis (102 aa), FASTA scores: opt: 413, E(): 3.7e-19, (66.7% identity in 102 aa overlap); etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Q7DA36 No PE/PPE ML2534 MMAR_0544 MSMEG_0618 Mb0293 NC_000962.3 Mycobrowser_v2 CDS 349624 349932 . + 0 Rv0285 PE5 Function unknown PE family protein PE5 Rv0285, (MTV035.13), len: 102 aa. PE5, Member of the Mycobacterium tuberculosis PE family (see Brennan & Delogu 2002), similar to others e.g. AL0212|MTV012_37 from Mycobacterium tuberculosis (105 aa), FASTA scores: opt: 497, E(): 2.6e-24, (80.4% identity in 102 aa overlap); Z80108|MTCY21B4.03 from Mycobacterium tuberculosis (102 aa), FASTA scores: opt: 413, E(): 3.7e-19, (66.7% identity in 102 aa overlap); etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Q7DA36 No PE/PPE ML2534 MMAR_0544 MSMEG_0618 Mb0293 NC_000962.3 Mycobrowser_v2 RBS 349924 349928 . + 0 Rv0286 PPE4 Function unknown PPE family protein PPE4 Rv0286, (MTV035.14), len: 513 aa. PPE4, Member of the Mycobacterium tuberculosis PPE family, similar to others e.g. AL0212|MTV012_32 from Mycobacterium tuberculosis (434 aa), FASTA scores: opt: 958, E(): 0, (43.5% identity in 522 aa overlap). Q7DA35 No PE/PPE ML2533 MMAR_0545 MSMEG_0619 Mb0294 NC_000962.3 Mycobrowser_v2 CDS 349935 351476 . + 0 Rv0286 PPE4 Function unknown PPE family protein PPE4 Rv0286, (MTV035.14), len: 513 aa. PPE4, Member of the Mycobacterium tuberculosis PPE family, similar to others e.g. AL0212|MTV012_32 from Mycobacterium tuberculosis (434 aa), FASTA scores: opt: 958, E(): 0, (43.5% identity in 522 aa overlap). Q7DA35 No PE/PPE ML2533 MMAR_0545 MSMEG_0619 Mb0294 NC_000962.3 Mycobrowser_v2 RBS 351512 351517 . + 0 Rv0287 esxG Function unknown ESAT-6 like protein EsxG (conserved protein TB9.8) Rv0287, (MTV035.15), len: 97 aa. EsxG, ESAT-6 like protein. PE-family related protein; distant member of the Mycobacterium tuberculosis PE family, similar to Rv3020c|AL0212|MTV012.34 (97 aa), FASTA scores: opt: 564, E(): 0, (91.8% identity in 97 aa overlap). Contains probable helix-turn-helix motif at aa 14-35 (Score 144, +4.11 SD). Seems to belong to the ESAT6 family (see Gey Van Pittius et al., 2001). Note that previously known as TB9.8. O53692 No cell wall and cell processes ML2532 MMAR_0546,MMAR_3001 MSMEG_0620 Mb0295 NC_000962.3 Mycobrowser_v2 CDS 351525 351818 . + 0 Rv0287 esxG Function unknown ESAT-6 like protein EsxG (conserved protein TB9.8) Rv0287, (MTV035.15), len: 97 aa. EsxG, ESAT-6 like protein. PE-family related protein; distant member of the Mycobacterium tuberculosis PE family, similar to Rv3020c|AL0212|MTV012.34 (97 aa), FASTA scores: opt: 564, E(): 0, (91.8% identity in 97 aa overlap). Contains probable helix-turn-helix motif at aa 14-35 (Score 144, +4.11 SD). Seems to belong to the ESAT6 family (see Gey Van Pittius et al., 2001). Note that previously known as TB9.8. O53692 No cell wall and cell processes ML2532 MMAR_0546,MMAR_3001 MSMEG_0620 Mb0295 NC_000962.3 Mycobrowser_v2 CDS 2274569 2275408 . - 0 Rv2028c Rv2028c Unknown Universal stress protein family protein Rv2028c, (MTV018.15c), len: 279 aa. Universal stress protein family protein, highly similar to many, contains IPR006016 UspA domain. O53474 No virulence, detoxification, adaptation MSMEG_5245 Mb2053c NC_000962.3 Mycobrowser_v2 RBS 2275416 2275420 . - 0 Rv2028c Rv2028c Unknown Universal stress protein family protein Rv2028c, (MTV018.15c), len: 279 aa. Universal stress protein family protein, highly similar to many, contains IPR006016 UspA domain. O53474 No virulence, detoxification, adaptation MSMEG_5245 Mb2053c NC_000962.3 Mycobrowser_v2 CDS 358945 359748 . - 0 Rv0295c Rv0295c Function unknown Conserved protein Rv0295c, (MTV035.23c), len: 267 aa. Conserved protein, showing weak similarity with CAC46877.1|AL591790 conserved hypothetical protein from Sinorhizobium meliloti (213 aa); and NP_104818.1|14023999|BAB50604.1|AP00300 Protein with weak similarity to NodH from Mesorhizobium loti (257 aa). Predicted to be an outer membrane protein (See Song et al., 2008). O53699 No conserved hypotheticals ML2526 MMAR_4255 MSMEG_0630 Mb0303c NC_000962.3 Mycobrowser_v2 CDS 352149 353036 . + 0 Rv0289 espG3 Function unknown ESX-3 secretion-associated protein EspG3 Rv0289, (MTV035.17), len: 295 aa. EspG3, ESX-3 secretion-associated protein, equivalent to CAC32061.1|AL583926 possible DNA-binding protein from Mycobacterium leprae (289 aa); and showing some similarity to Rv3866|G70656|CAB06238.1|Z94121|MTCY15F10.23 from Mycobacterium tuberculosis (276 aa), FASTA scores: opt: 149, E(): 0.0035, (27.7% identity in 289 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53694 No cell wall and cell processes ML2530c MMAR_0548 MSMEG_0622 Mb0297 NC_000962.3 Mycobrowser_v2 RBS 3315224 3315228 . + 0 Rv2963 Rv2963 Unknown Probable integral membrane protein Rv2963, (MTCY349.24c), len: 406 aa. Probable integral membrane protein. P95129 No cell wall and cell processes ML1662 MMAR_1754 Mb2987 NC_000962.3 Mycobrowser_v2 CDS 3315236 3316456 . + 0 Rv2963 Rv2963 Unknown Probable integral membrane protein Rv2963, (MTCY349.24c), len: 406 aa. Probable integral membrane protein. P95129 No cell wall and cell processes ML1662 MMAR_1754 Mb2987 NC_000962.3 Mycobrowser_v2 RBS 353067 353070 . + 0 Rv0290 eccD3 Unknown ESX conserved component EccD3. ESX-3 type VII secretion system protein. Probable transmembrane protein. Rv0290, (MTV035.18), len: 472 aa. EccD3, esx conserved component, ESX-3 type VII secretion system protein, probable transmembrane protein, similar to several others in mycobacteria e.g. Z95389|MTCY77_20|Rv3887c from Mycobacterium tuberculosis (467 aa), FASTA scores: opt: 429, E(): 5.1e-19, (28. 6% identity in 479 aa overlap); Rv3877; Rv1795; Rv3448; and Y14967|MLCB628_9|MLCB628.10c from Mycobacterium leprae (480 aa), FASTA scores: opt: 269, E(): 3.1e-09, (26.0% identity in 503 aa overlap). O86362 No cell wall and cell processes ML2529c MMAR_0549 MSMEG_0623 Mb0298 NC_000962.3 Mycobrowser_v2 CDS 353083 354501 . + 0 Rv0290 eccD3 Unknown ESX conserved component EccD3. ESX-3 type VII secretion system protein. Probable transmembrane protein. Rv0290, (MTV035.18), len: 472 aa. EccD3, esx conserved component, ESX-3 type VII secretion system protein, probable transmembrane protein, similar to several others in mycobacteria e.g. Z95389|MTCY77_20|Rv3887c from Mycobacterium tuberculosis (467 aa), FASTA scores: opt: 429, E(): 5.1e-19, (28. 6% identity in 479 aa overlap); Rv3877; Rv1795; Rv3448; and Y14967|MLCB628_9|MLCB628.10c from Mycobacterium leprae (480 aa), FASTA scores: opt: 269, E(): 3.1e-09, (26.0% identity in 503 aa overlap). O86362 No cell wall and cell processes ML2529c MMAR_0549 MSMEG_0623 Mb0298 NC_000962.3 Mycobrowser_v2 CDS 354498 355883 . + 0 Rv0291 mycP3 Thought to have proteolytic activity. Probable membrane-anchored mycosin MycP3 (serine protease) (subtilisin-like protease) (subtilase-like) (mycosin-3) Rv0291, (MTV035.19), len: 461 aa. Probable mycP3, membrane-anchored serine protease (mycosin) (see Brown et al., 2000), similar to several others in mycobacteria e.g. Z94121|MTY15F10_28|Rv1796 from Mycobacterium tuberculosis (446 aa), FASTA scores: opt: 1168, E(): 0, (44.6% identity in 453 aa overlap); Rv3886c; Rv3883c; Rv3449; and Y14967|MLCB628_4|MLCB628.04 from Mycobacterium leprae (446 aa), FASTA scores: opt: 1159, E(): 0, (43.5 identity in 446 aa overlap). Has signal sequence and hydrophobic stretch at C-terminus, followed by short positively charged segment, that seems to act as a membrane anchor. Contains PS00137 Serine proteases, subtilase family, histidine active site signature. Belongs to peptidase family S8 (also known as the subtilase family), pyrolysin subfamily. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). O53695 No intermediary metabolism and respiration ML2528 MMAR_0550 MSMEG_0624 Mb0299 NC_000962.3 Mycobrowser_v2 RBS 355868 355871 . + 0 Rv0292 eccE3 Unknown ESX conserved component EccE3. ESX-3 type VII secretion system protein. Probable transmembrane protein. Rv0292, (MTV035.20), len: 331 aa. EccE3, esx conserved component, ESX-3 type VII secretion system protein, probable transmembrane protein (has two hydrophobic segments at N-terminal end), equivalent to CAC32058.1|AL583926 conserved membrane protein from Mycobacterium leprae (339 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53696 No cell wall and cell processes ML2527c MMAR_0551 MSMEG_0626 Mb0300 NC_000962.3 Mycobrowser_v2 CDS 355880 356875 . + 0 Rv0292 eccE3 Unknown ESX conserved component EccE3. ESX-3 type VII secretion system protein. Probable transmembrane protein. Rv0292, (MTV035.20), len: 331 aa. EccE3, esx conserved component, ESX-3 type VII secretion system protein, probable transmembrane protein (has two hydrophobic segments at N-terminal end), equivalent to CAC32058.1|AL583926 conserved membrane protein from Mycobacterium leprae (339 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53696 No cell wall and cell processes ML2527c MMAR_0551 MSMEG_0626 Mb0300 NC_000962.3 Mycobrowser_v2 CDS 356862 358064 . - 0 Rv0293c Rv0293c Function unknown Conserved protein Rv0293c, (MTV035.21c), len: 400 aa. Conserved protein, similar in C-terminal part to Rv2627c|B70573|MTCY01A10.05|CAB08637.1|Z95387 conserved hypothetical protein from Mycobacterium tuberculosis (413 aa), FASTA scores: opt: 394, E(): 2.1e-17, (31.1% identity in 299 aa overlap). O53697 No conserved hypotheticals MMAR_0552 MSMEG_0625 Mb0301c NC_000962.3 Mycobrowser_v2 CDS 442395 443078 . + 0 Rv0364 Rv0364 Unknown Possible conserved transmembrane protein Rv0364, (MTCY13E10.26), len: 227 aa. Possible conserved transmembrane protein, equivalent to O69601|Y364_MYCLE|ML0287|CAA18951.1|AL023514|AL023514|MLCB4_19 hypothetical 24.3 KDA protein from Mycobacterium leprae (222 aa), FASTA scores: opt: 1027, E(): 0, (66.1% identity in 227 aa overlap). Shows strong similarity to DEDA_ECOLI|P09548 DedA protein protein from Escherichia coli FASTA scores: E(): 1.3e-28, (39.5% identity in 195 aa overlap). Similar also to Mycobacterium tuberculosis DedA protein Rv2637|MTCY441.0. O06314 No cell wall and cell processes ML0287 MMAR_0668 Mb0371 NC_000962.3 Mycobrowser_v2 RBS 358159 358163 . + 0 Rv0294 tam Possibly catalyzes the S-adenosylmethionine monomethyl esterification of trans-aconitate at high affinity and of cis-aconitate, isocitrate, and citrate at lower velocities and affinities. Probable trans-aconitate methyltransferase Tam Rv0294, (MTV035.22), len: 261 aa. Probable tam, trans-aconitate methyltransferase, similar to others e.g. P76145|TAM_ECOLI|7465793|B64906|B1519 trans-aconitate methyltransferase from Escherichia coli strain K12 (252 aa), FASTA scores: opt: 649, E(): 0, (39.3 identity in 252 aa overlap). Belongs to the methyltransferase superfamily. P66885 No intermediary metabolism and respiration MMAR_0553 MSMEG_0629 Mb0302 NC_000962.3 Mycobrowser_v2 CDS 358171 358956 . + 0 Rv0294 tam Possibly catalyzes the S-adenosylmethionine monomethyl esterification of trans-aconitate at high affinity and of cis-aconitate, isocitrate, and citrate at lower velocities and affinities. Probable trans-aconitate methyltransferase Tam Rv0294, (MTV035.22), len: 261 aa. Probable tam, trans-aconitate methyltransferase, similar to others e.g. P76145|TAM_ECOLI|7465793|B64906|B1519 trans-aconitate methyltransferase from Escherichia coli strain K12 (252 aa), FASTA scores: opt: 649, E(): 0, (39.3 identity in 252 aa overlap). Belongs to the methyltransferase superfamily. P66885 No intermediary metabolism and respiration MMAR_0553 MSMEG_0629 Mb0302 NC_000962.3 Mycobrowser_v2 CDS 359758 361155 . - 0 Rv0296c Rv0296c Function unknown; probably involved in cellular metabolism. Probable sulfatase Rv0296c, (MTCY63.01c, MTV035.24c), len: 465 aa. Probable sulfatase, possibly an aryl-/steryl-sulfatase or a sulfamidase (sulfohydrolase) (sulphamidase). Similar to various hydrolases e.g. AAG41945.1|AF304053_1|AF304053 heparan N-sulfatase from Mus musculus (502 aa); NP_061292.1|6851181|AAF29460.1|AF153827_1|AF153827 N-sulfoglucosamine sulfohydrolase (sulfamidase) (sulphamidase) from Mus musculus (502 aa); AAG17206.1|AF217203_1|AF217203 heparan sulfate sulfamidase from Canis familiaris (507 aa); P08842|STS_HUMAN|1360652 steryl-sulfatase precursor (steroid sulfatase) (steryl-sulfate sulfohydrolase) (arylsulfatase C) (ASC) from Homo sapiens (583 aa); ARSB_FELCA|P33727 arylsulfatase B precursor (535 aa), FASTA scores: opt: 231, E(): 1.7e-08, (30.3% identity in 261 aa overlap). Also similarity with 4 others sulfatases in Mycobacterium tuberculosis. Contains sulfatases signature 1 (PS00523). Note that previously known as atsG. Q6MX51 No intermediary metabolism and respiration ML2525 MMAR_0554 Mb0304c NC_000962.3 Mycobrowser_v2 RBS 361167 361170 . - 0 Rv0296c Rv0296c Function unknown; probably involved in cellular metabolism. Probable sulfatase Rv0296c, (MTCY63.01c, MTV035.24c), len: 465 aa. Probable sulfatase, possibly an aryl-/steryl-sulfatase or a sulfamidase (sulfohydrolase) (sulphamidase). Similar to various hydrolases e.g. AAG41945.1|AF304053_1|AF304053 heparan N-sulfatase from Mus musculus (502 aa); NP_061292.1|6851181|AAF29460.1|AF153827_1|AF153827 N-sulfoglucosamine sulfohydrolase (sulfamidase) (sulphamidase) from Mus musculus (502 aa); AAG17206.1|AF217203_1|AF217203 heparan sulfate sulfamidase from Canis familiaris (507 aa); P08842|STS_HUMAN|1360652 steryl-sulfatase precursor (steroid sulfatase) (steryl-sulfate sulfohydrolase) (arylsulfatase C) (ASC) from Homo sapiens (583 aa); ARSB_FELCA|P33727 arylsulfatase B precursor (535 aa), FASTA scores: opt: 231, E(): 1.7e-08, (30.3% identity in 261 aa overlap). Also similarity with 4 others sulfatases in Mycobacterium tuberculosis. Contains sulfatases signature 1 (PS00523). Note that previously known as atsG. Q6MX51 No intermediary metabolism and respiration ML2525 MMAR_0554 Mb0304c NC_000962.3 Mycobrowser_v2 RBS 361324 361328 . + 0 Rv0297 PE_PGRS5 Function unknown PE-PGRS family protein PE_PGRS5 Rv0297, (MTCY63.02), len: 591 aa. PE_PGRS5, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to others e.g. Y03A_MYCTU|Q10637 from Mycobacterium tuberculosis (603 aa), FASTA scores: opt: 1884, E(): 0, (53.7% identity in 635 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q6MX50 No PE/PPE MMAR_4149 Mb0305 NC_000962.3 Mycobrowser_v2 CDS 361334 363109 . + 0 Rv0297 PE_PGRS5 Function unknown PE-PGRS family protein PE_PGRS5 Rv0297, (MTCY63.02), len: 591 aa. PE_PGRS5, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to others e.g. Y03A_MYCTU|Q10637 from Mycobacterium tuberculosis (603 aa), FASTA scores: opt: 1884, E(): 0, (53.7% identity in 635 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q6MX50 No PE/PPE MMAR_4149 Mb0305 NC_000962.3 Mycobrowser_v2 RBS 363240 363243 . + 0 Rv0298 Rv0298 Unknown Hypothetical protein Rv0298, (MTCY63.03), len: 75 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07225 No conserved hypotheticals Mb0306 NC_000962.3 Mycobrowser_v2 CDS 363252 363479 . + 0 Rv0298 Rv0298 Unknown Hypothetical protein Rv0298, (MTCY63.03), len: 75 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07225 No conserved hypotheticals Mb0306 NC_000962.3 Mycobrowser_v2 CDS 363476 363778 . + 0 Rv0299 Rv0299 Unknown Hypothetical protein Rv0299, (MTCY63.04), len: 100 aa. Hypothetical unknown protein. Equivalent to AAK44536.1 from Mycobacterium tuberculosis strain CDC1551 (49 aa) but longer 51 aa. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07226 No conserved hypotheticals Mb0307 NC_000962.3 Mycobrowser_v2 CDS 392273 392728 . + 0 Rv0326 Rv0326 Unknown Hypothetical protein Rv0326, (MTCY63.31), len: 151 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07251 No unknown Mb0333 NC_000962.3 Mycobrowser_v2 CDS 363826 364047 . + 0 Rv0300 vapB2 Unknown Possible antitoxin VapB2 Rv0300, (MTCY63.05), len: 73 aa. Possible vapB2, antitoxin, part of toxin-antitoxin (TA) operon with Rv0301 (See Arcus et al., 2005; Pandey and Gerdes, 2005). Weak similarity with others e.g. Rv3697c from Mycobacterium tuberculosis (145 aa), Rv1721c|MTCY04C12.06c|Z81360|MTCY4C12_4 conserved hypothetical protein from Mycobacterium tuberculosis (75 aa), FASTA scores: opt: 84, E(): 8.3, (39.5% identity in 38 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07227 No virulence, detoxification, adaptation Mb0308 NC_000962.3 Mycobrowser_v2 RBS 364590 364593 . + 0 Rv0302 Rv0302 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably TetR/AcrR-family) Rv0302, (MTCY63.07), len: 210 aa. Probable transcription regulatory protein, TetR family (see citation below), with its N-terminus similar to N-terminus of several repressors and regulatory proteins of TetR/AcrR family e.g. ACRR_ECOLI|P34000 potential acraB operon repressor from Escherichia coli (215 aa), FASTA scores: opt: 172, E(): 3.1e-05, (22.7% identity in 194 aa overlap). Also similar in N-terminus to N-terminus of MTCY07A7.24 hypothetical regulator from Mycobacterium tuberculosis FASTA score: (38.7% identity in 62 aa overlap). Contains probable helix-turn helix motif from aa 35-56 (Score 1728, +5.07 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07229 No regulatory proteins MMAR_1504 Mb0310 NC_000962.3 Mycobrowser_v2 CDS 364605 365237 . + 0 Rv0302 Rv0302 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably TetR/AcrR-family) Rv0302, (MTCY63.07), len: 210 aa. Probable transcription regulatory protein, TetR family (see citation below), with its N-terminus similar to N-terminus of several repressors and regulatory proteins of TetR/AcrR family e.g. ACRR_ECOLI|P34000 potential acraB operon repressor from Escherichia coli (215 aa), FASTA scores: opt: 172, E(): 3.1e-05, (22.7% identity in 194 aa overlap). Also similar in N-terminus to N-terminus of MTCY07A7.24 hypothetical regulator from Mycobacterium tuberculosis FASTA score: (38.7% identity in 62 aa overlap). Contains probable helix-turn helix motif from aa 35-56 (Score 1728, +5.07 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07229 No regulatory proteins MMAR_1504 Mb0310 NC_000962.3 Mycobrowser_v2 RBS 365225 365229 . + 0 Rv0303 Rv0303 Function unknown; probably involved in cellular metabolism. Probable dehydrogenase/reductase Rv0303, (MTCY63.08), len: 302 aa. Possible dehydrogenase/reductase, similar to various NADPH dehydrogenases and other NADPH oxidoreductases e.g. O48741|PORC_ARATH|7488284|T00897 protochlorophyllide reductase C chloroplast precursor (NADPH-protochlorophyllide oxidoreductase C) from Arabidopsis thaliana (401 aa); Q42850 NADPH dehydrogenase (395 aa), FASTA scores: opt: 347, E(): 3.8e-16, (35.4% identity in 319 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07230 No intermediary metabolism and respiration Mb0311 NC_000962.3 Mycobrowser_v2 CDS 365234 366142 . + 0 Rv0303 Rv0303 Function unknown; probably involved in cellular metabolism. Probable dehydrogenase/reductase Rv0303, (MTCY63.08), len: 302 aa. Possible dehydrogenase/reductase, similar to various NADPH dehydrogenases and other NADPH oxidoreductases e.g. O48741|PORC_ARATH|7488284|T00897 protochlorophyllide reductase C chloroplast precursor (NADPH-protochlorophyllide oxidoreductase C) from Arabidopsis thaliana (401 aa); Q42850 NADPH dehydrogenase (395 aa), FASTA scores: opt: 347, E(): 3.8e-16, (35.4% identity in 319 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07230 No intermediary metabolism and respiration Mb0311 NC_000962.3 Mycobrowser_v2 CDS 366150 372764 . - 0 Rv0304c PPE5 Function unknown PPE family protein PPE5 Rv0304c, (MTCY63.9c), len: 2204 aa. PPE5, Member of the Mycobacterium tuberculosis PE family (PPE, MPTR), similar to others e.g. Z95324|MTY13E10_16 from Mycobacterium tuberculosis (1443 aa), FASTA scores: E(): 0, (50.6% identity in 1403 aa overlap); Y04H_MYCTU|Q10778 from Mycobacterium tuberculosis (734 aa), FASTA scores: opt: 989, E(): 0, (42.3% identity in 522 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q6MX49 No PE/PPE Mb0312c,Mb0313c NC_000962.3 Mycobrowser_v2 CDS 397442 398227 . + 0 Rv0332 Rv0332 Function unknown Conserved protein Rv0332, (MTCY63.37), len: 261 aa. Conserved protein, similar to several conserved hypothetical proteins from Streptomyces coelicolor e.g. SC6A9.18c|AL031035|SC6A9_18|T35449 hypothetical protein (266 aa), FASTA scores: opt: 508, E(): 5.7e-27, (36.7% identity in 251 aa overlap). O07256 No conserved hypotheticals ML2505 MMAR_0604 MSMEG_0682 Mb0339 NC_000962.3 Mycobrowser_v2 CDS 372820 375711 . - 0 Rv0305c PPE6 Function unknown PPE family protein PPE6 Rv0305c, (MTCY63.10c), len: 963 aa. PPE6, Member of the Mycobacterium tuberculosis PE family (PPE, MPTR), similar to others e.g. Y04H_MYCTU|Q10778 from Mycobacterium tuberculosis (734 aa), FASTA scores: opt: 1340, E(): 0, (40.9% identity in 815 aa overlap). Q6MX48 No PE/PPE Mb0313c NC_000962.3 Mycobrowser_v2 CDS 375914 376585 . + 0 Rv0306 Rv0306 Function unknown; probably involved in cellular metabolism. Putative oxidoreductase Rv0306, (MTCY63.11), len: 223 aa. Putative oxidoreductase, highly similar to H83485|9947208|AAG04663.1|AE004557_4|AE004557 conserved hypothetical protein from Pseudomonas aeruginosa strain PAO1 (218 aa); and to other putative oxidoreductases e.g. middle part of CAB76073.1|AL157953 putative nitroreductase from Streptomyces coelicolor (1212 aa); Q52685|BLUB protein involved in cobalamin (vitamin B12) synthesis from Rhodobacter capsulatus (206 aa), FASTA scores: opt: 318, E(): 2e-15, (35.6% identity in 191 aa overlap). O07233 No intermediary metabolism and respiration MMAR_0555 MSMEG_6053 Mb0314 NC_000962.3 Mycobrowser_v2 RBS 377101 377105 . + 0 Rv0308 Rv0308 Unknown Probable conserved integral membrane protein Rv0308, (MTCY63.13), len: 238 aa. Probable conserved integral membrane protein, with C-terminus highly similar to C-terminus of other integral membrane proteins or phosphatases e.g. AAK25788.1|AF336822_1|13430250|AAK25789.1|AF336823_1 putative phosphatase from Streptococcus pyogenes (201 aa); Q06074 hypothetical 24.9 kDa protein (216 aa), FASTA scores: opt: 209, E(): 2e-07, (27.9% identity in 140 aa overlap). Could be a phosphatase. O07235 No cell wall and cell processes ML2523 MMAR_0558 MSMEG_0634 Mb0316 NC_000962.3 Mycobrowser_v2 CDS 377113 377829 . + 0 Rv0308 Rv0308 Unknown Probable conserved integral membrane protein Rv0308, (MTCY63.13), len: 238 aa. Probable conserved integral membrane protein, with C-terminus highly similar to C-terminus of other integral membrane proteins or phosphatases e.g. AAK25788.1|AF336822_1|13430250|AAK25789.1|AF336823_1 putative phosphatase from Streptococcus pyogenes (201 aa); Q06074 hypothetical 24.9 kDa protein (216 aa), FASTA scores: opt: 209, E(): 2e-07, (27.9% identity in 140 aa overlap). Could be a phosphatase. O07235 No cell wall and cell processes ML2523 MMAR_0558 MSMEG_0634 Mb0316 NC_000962.3 Mycobrowser_v2 CDS 377931 378587 . + 0 Rv0309 Rv0309 Unknown Possible conserved exported protein Rv0309, (MTCY63.14), len: 218 aa. Possible conserved exported protein (has putative N-terminal signal sequence), equivalent to AC32053.1|AL583926 putative secreted protein from Mycobacterium leprae (218 aa). Also similar to others e.g. AB76092.1|AL157956 putative secreted protein from Streptomyces coelicolor (238 aa). Predicted to be an outer membrane protein (See Song et al., 2008). O07236 No cell wall and cell processes ML2522 MMAR_0559 MSMEG_0635 Mb0317 NC_000962.3 Mycobrowser_v2 CDS 378657 379148 . - 0 Rv0310c Rv0310c Function unknown Conserved protein Rv0310c, (MTCY63.15c), len: 163 aa. Conserved protein, similar to some bile acid dehydratases e.g. P19412|BAIE_EUBSP|98749|D37844|1381566|AAC45413.1|U57489 bile acid-inducible operon protein E from Eubacterium sp (166 aa), FASTA scores: opt: 302, E(): 1e-11, (38.8% identity in 134 aa overlap); AAF22847.1|AF210152_4 bile acid 7a-dehydratase from Clostridium sp. (168 aa). O07237 No conserved hypotheticals ML2521 MMAR_0560 MSMEG_0636 Mb0318c NC_000962.3 Mycobrowser_v2 CDS 379172 380401 . + 0 Rv0311 Rv0311 Unknown Unknown protein Rv0311, (MTCY63.16), len: 409 aa. Unknown protein. Contains PS00881 Protein splicing signature. O07238 No conserved hypotheticals ML2520 MMAR_0561 MSMEG_5855 Mb0319 NC_000962.3 Mycobrowser_v2 CDS 398254 398628 . + 0 Rv0333 Rv0333 Unknown Unknown protein Rv0333, (MTCY63.38), len: 124 aa. Unknown protein. O33273 No conserved hypotheticals ML2504 MMAR_0605 MSMEG_0683 Mb0340 NC_000962.3 Mycobrowser_v2 CDS 380556 382418 . + 0 Rv0312 Rv0312 Function unknown Conserved hypothetical proline and threonine rich protein Rv0312, (MTCY63.17), len: 620 aa. Conserved hypothetical protein with highly Pro-, Thr-rich C-terminus. Similar to Pro-,Thr-rich region in Rv2264c|AL021925|MTV022_14 from Mycobacterium tuberculosis (592 aa), FASTA scores: opt: 1075, E(): 0, (38.9% identity in 627 aa overlap). Also some similarity with Rv0350|dnaK from Mycobacterium tuberculosis. Possibly membrane protein; has hydrophobic stetch in its middle part. O07239 No conserved hypotheticals ML2519 MMAR_0562 MSMEG_0638 Mb0320 NC_000962.3 Mycobrowser_v2 CDS 383602 384486 . + 0 Rv0315 Rv0315 Possibly hydrolyzes specific sugar (hydrolyzation of glycosidic bond) and could be involved in exopolysaccharide biosynthesis/degradation. Could also have a LYTIC activity against cell WALLS. Possible beta-1,3-glucanase precursor Rv0315, (MTCY63.20), len: 294 aa. Possible beta-1,3-glucanase precursor (has hydrophobic stretch in its N-terminal part), similar to others e.g. Q51333|AAC44371.1 beta-1,3-glucanase II a from Oerskovia xanthineolytica (306 aa), FASTA scores: opt: 76, E(): 3e-14, (34.1% identity in 302 aa overlap); and AAC38290.1|AF052745 beta-1,3-glucanase II from Oerskovia xanthineolytica (435 aa). Contains glycosyl hydrolases family 16 active site signature (PS01034). O07242 No intermediary metabolism and respiration ML2516 MMAR_0567 MSMEG_0645 Mb0323 NC_000962.3 Mycobrowser_v2 RBS 384526 384529 . + 0 Rv0316 Rv0316 Could be involved in the catabolism of catechol to succinate- and acetyl-CoA in the beta-ketoadipate pathway (at the third step) [catalytic activity: 2,5-dihydro-5-oxofuran-2-acetate = 3,4-dihydro-5-oxofuran-2-acetate]. Possible muconolactone isomerase Rv0316, (MTCY63.21), len: 204 aa. Possible muconolactone isomerase, showing weak similarity with some muconolactone isomerases e.g. O33947|CTC1_ACILW muconolactone delta-isomerase 1 (MIASE 1)(96 aa), FASTA scores: opt: 179, E(): 3.9e-05, (32.6% identity in 92 aa overlap). O07243 No intermediary metabolism and respiration MMAR_0568 Mb0324 NC_000962.3 Mycobrowser_v2 CDS 384535 385149 . + 0 Rv0316 Rv0316 Could be involved in the catabolism of catechol to succinate- and acetyl-CoA in the beta-ketoadipate pathway (at the third step) [catalytic activity: 2,5-dihydro-5-oxofuran-2-acetate = 3,4-dihydro-5-oxofuran-2-acetate]. Possible muconolactone isomerase Rv0316, (MTCY63.21), len: 204 aa. Possible muconolactone isomerase, showing weak similarity with some muconolactone isomerases e.g. O33947|CTC1_ACILW muconolactone delta-isomerase 1 (MIASE 1)(96 aa), FASTA scores: opt: 179, E(): 3.9e-05, (32.6% identity in 92 aa overlap). O07243 No intermediary metabolism and respiration MMAR_0568 Mb0324 NC_000962.3 Mycobrowser_v2 CDS 385173 385943 . - 0 Rv0317c glpQ2 Glycerophosphoryl diester phosphodiesterase hydrolyzes deacylated phospholipids to G3P and the corresponding alcohols [catalytic activity: a glycerophosphodiester + H(2)O = an alcohol + SN-glycerol 3-phosphate]. Possible glycerophosphoryl diester phosphodiesterase GlpQ2 (glycerophosphodiester phosphodiesterase) Rv0317c, (MTCY63.22c), len: 256 aa (start uncertain, chosen by homology). Possible glpQ2, glycerophosphoryl diester phosphodiesterase, similar to others e.g. E75317|6459876|AAF11631.1|AE002044_4 glycerophosphoryl diester phosphodiesterase from Deinococcus radiodurans (285 aa); P10908|UGPQ_ECOLI from Escherichia coli (247 aa), FASTA scores: opt: 220, E(): 5.2e-07, (28.0% identity in 250 aa overlap). Also similar to MTCY01A6.27 from Mycobacterium tuberculosis FASTA score: (27.5% identity in 247 aa overlap). O07244 No intermediary metabolism and respiration MMAR_0494 Mb0325c NC_000962.3 Mycobrowser_v2 RBS 385954 385958 . - 0 Rv0317c glpQ2 Glycerophosphoryl diester phosphodiesterase hydrolyzes deacylated phospholipids to G3P and the corresponding alcohols [catalytic activity: a glycerophosphodiester + H(2)O = an alcohol + SN-glycerol 3-phosphate]. Possible glycerophosphoryl diester phosphodiesterase GlpQ2 (glycerophosphodiester phosphodiesterase) Rv0317c, (MTCY63.22c), len: 256 aa (start uncertain, chosen by homology). Possible glpQ2, glycerophosphoryl diester phosphodiesterase, similar to others e.g. E75317|6459876|AAF11631.1|AE002044_4 glycerophosphoryl diester phosphodiesterase from Deinococcus radiodurans (285 aa); P10908|UGPQ_ECOLI from Escherichia coli (247 aa), FASTA scores: opt: 220, E(): 5.2e-07, (28.0% identity in 250 aa overlap). Also similar to MTCY01A6.27 from Mycobacterium tuberculosis FASTA score: (27.5% identity in 247 aa overlap). O07244 No intermediary metabolism and respiration MMAR_0494 Mb0325c NC_000962.3 Mycobrowser_v2 CDS 386305 387099 . - 0 Rv0318c Rv0318c Unknown Probable conserved integral membrane protein Rv0318c, (MTCY63.23c), len: 264 aa. Probable conserved integral membrane protein, with some similarity to C-terminus of GUFA_MYXXA|Q06916 (254 aa), FASTA scores: opt: 157, E (): 0.0032, (28.3% identity in 198 aa overlap). Also similar to O26573 conserved protein from Methanobacterium thermoauto (259 aa), FASTA scores: opt: 173, E(): 5.2e-05, (32.7% identity in 214 aa overlap). Q6MX47 No cell wall and cell processes MMAR_0595 Mb0326c NC_000962.3 Mycobrowser_v2 RBS 387133 387138 . + 0 Rv0319 pcp Removes 5-oxoproline from various penultimate amino acid residues except L-proline [catalytic activity: 5-oxoprolyl-peptide + H2O = 5-oxoproline + peptide]. Probable pyrrolidone-carboxylate peptidase Pcp (5-oxoprolyl-peptidase) (pyroglutamyl-peptidase I) (PGP-I) (pyrase) Rv0319, (MTCY63.24), len: 222 aa. Probable pcp, pyrrolidone-carboxylate peptidase, highly similar to others e.g. PCP_PSEFL|P42673 pyrrolidone-carboxylate peptidase from Pseudomonas fluorescens (213 aa), FASTA scores: opt: 478, E(): 7.5e-25, (40.2% identity in 219 aa overlap). Belongs to peptidase family C15 (thiol protease). P0A5R4 No intermediary metabolism and respiration MMAR_0598 Mb0327 NC_000962.3 Mycobrowser_v2 CDS 387148 387816 . + 0 Rv0319 pcp Removes 5-oxoproline from various penultimate amino acid residues except L-proline [catalytic activity: 5-oxoprolyl-peptide + H2O = 5-oxoproline + peptide]. Probable pyrrolidone-carboxylate peptidase Pcp (5-oxoprolyl-peptidase) (pyroglutamyl-peptidase I) (PGP-I) (pyrase) Rv0319, (MTCY63.24), len: 222 aa. Probable pcp, pyrrolidone-carboxylate peptidase, highly similar to others e.g. PCP_PSEFL|P42673 pyrrolidone-carboxylate peptidase from Pseudomonas fluorescens (213 aa), FASTA scores: opt: 478, E(): 7.5e-25, (40.2% identity in 219 aa overlap). Belongs to peptidase family C15 (thiol protease). P0A5R4 No intermediary metabolism and respiration MMAR_0598 Mb0327 NC_000962.3 Mycobrowser_v2 CDS 388582 389154 . + 0 Rv0321 dcd Involved in interconversion of dCTP and dUTP [catalytic activity: dCTP + H2O = dUTP + NH3]. Probable deoxycytidine triphosphate deaminase Dcd (dCTP deaminase) Rv0321, (MTCY63.26), len: 190 aa. Probable dcd (alternate gene names: dus or paxA), deoxycytidine triphosphate deaminase, equivalent to CAC32024.1|AL583925 probable deoxycytidine triphosphate deaminase from Mycobacterium leprae (190 aa). Also highly similar to others e.g. Q9X8W0|DCD_STRCO|7480599|T36613|SCH35.46 deoxycytidine triphosphate deaminase from Streptomyces coelicolor (191 aa); DCD_ECOLI|P28248|DUS|PAXA|B2065 deoxycytidine triphosphate deaminase from Escherichia coli strain K12 (193 aa), FASTA scores: opt: 408, E(): 2.7e-21, (43.1% identity in 188 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the dCTP deaminase family. The transcription of this CDS seems to be activated specifically in host granulomas (see citation below). O07247 No intermediary metabolism and respiration ML2507 MMAR_0600 MSMEG_0678 Mb0329 NC_000962.3 Mycobrowser_v2 CDS 389260 390591 . + 0 Rv0322 udgA Possibly involved in polysaccharide biosynthesis [catalytic activity: UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH]. Probable UDP-glucose 6-dehydrogenase UdgA (UDP-GLC dehydrogenase) (UDP-GLCDH) (UDPGDH) Rv0322, (MTCY63.27), len: 443 aa. Probable udg (alternate gene name: rkpK), UDP-glucose 6-dehydrogenase , highly similar to others e.g. CAC44517.1|AL596138 putative UDP-glucose 6-dehydrogenase from Streptomyces coelicolor (447 aa); Q56812 UDP-glucose dehydrogenase from Xanthomonas campestris (445 aa), FASTA scores: opt: 713, E(): 0, (41.9% identity in 351 aa overlap); etc. Also similar to several GDP-mannose 6-dehydrogenase. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the UDP-glucose/GDP-mannose dehydrogenases family. O07248 No intermediary metabolism and respiration ML2506 MMAR_0603 MSMEG_0680 Mb0330 NC_000962.3 Mycobrowser_v2 CDS 390580 391251 . - 0 Rv0323c Rv0323c Function unknown Conserved hypothetical protein Rv0323c, (MTCY63.28c), len: 223 aa. Conserved hypothetical protein, similar to others e.g. YPJG_BACSU|P42981 hypothetical 24.8 kDa protein from Bacillus subtilis (224 aa), FASTA scores: opt: 182, E(): 1.3e-05, (27.5% identity in 211 aa overlap). Also some similarity to MLU15183_8 from Mycobacterium tuberculosis FASTA score: (32.0% identity in 147 aa overlap). Alternative nucleotide at position 390828 (T->C; S142G) has been observed. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07249 No conserved hypotheticals Mb0331c NC_000962.3 Mycobrowser_v2 RBS 391338 391342 . + 0 Rv0324 Rv0324 Possibly involved in transcriptional mechanism. Possible transcriptional regulatory protein (possibly ArsR-family) Rv0324, (MTCY63.29), len: 226 aa. Possible transcriptional regulator, arsR family, with its N-terminus similar to the N-terminus of other DNA-binding proteins e.g. P30346|MERR_STRLI probable mercury resistance operon from Streptomyces lividans (125 aa), FASTA scores: opt: 154, E(): 0.002, (32.2% identity in 90 aa overlap), and its C-terminal part similar to hypothetical bacterial proteins e.g. P54510|YQHL_BACSU hypothetical 14.6 kDa protein from Bacillus subtilis (126 aa), FASTA scores: opt: 159, E(): 0.00097, (35.5% identity in 76 aa overlap). Most similar to AJ005575|SPE005575_2 ORF1 required for antibiotic production from Streptomyces peucetius (226 aa), FASTA scores: opt: 816, E(): 0, (60.7% identity in 211 aa overlap). Also similar in C-terminus to MTCY164.26 molybdopterin biosynthesis moeb protein from Mycobacterium tuberculosis FASTA score: (36.8% identity in 114 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O08446 No regulatory proteins Mb0332 NC_000962.3 Mycobrowser_v2 CDS 391352 392032 . + 0 Rv0324 Rv0324 Possibly involved in transcriptional mechanism. Possible transcriptional regulatory protein (possibly ArsR-family) Rv0324, (MTCY63.29), len: 226 aa. Possible transcriptional regulator, arsR family, with its N-terminus similar to the N-terminus of other DNA-binding proteins e.g. P30346|MERR_STRLI probable mercury resistance operon from Streptomyces lividans (125 aa), FASTA scores: opt: 154, E(): 0.002, (32.2% identity in 90 aa overlap), and its C-terminal part similar to hypothetical bacterial proteins e.g. P54510|YQHL_BACSU hypothetical 14.6 kDa protein from Bacillus subtilis (126 aa), FASTA scores: opt: 159, E(): 0.00097, (35.5% identity in 76 aa overlap). Most similar to AJ005575|SPE005575_2 ORF1 required for antibiotic production from Streptomyces peucetius (226 aa), FASTA scores: opt: 816, E(): 0, (60.7% identity in 211 aa overlap). Also similar in C-terminus to MTCY164.26 molybdopterin biosynthesis moeb protein from Mycobacterium tuberculosis FASTA score: (36.8% identity in 114 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O08446 No regulatory proteins Mb0332 NC_000962.3 Mycobrowser_v2 CDS 392696 394045 . - 0 Rv0327c cyp135A1 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Possible cytochrome P450 135A1 Cyp135A1 Rv0327c, (MT0342, MTCY63.32c), len: 449 aa. Possible cyp135A1, cytochrome P450, similar to cytochrome P-450 monoxygenases and other cytochrome P-450 related enzymes e.g. FQ12609 putative P450 monooxygenase (506 aa), FASTA scores: opt: 276, E() : 1.7e-11, (27.9% identity in 433 aa overlap). Also similar to other Mycobacterium tuberculosis proteins e.g. MTV039.06|Rv0568 putative cytochrome P450 (472 aa); MTCI5.10 cytochrome p450 FASTA score: (30.4% identity in 434 aa overlap). Contains cytochrome P450 cysteine heme-iron ligand signature (PS00086). Belongs to the cytochrome P450 family. Alternative start possible at 33706 but no RBS. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O08447 No intermediary metabolism and respiration Mb0334c NC_000962.3 Mycobrowser_v2 RBS 394051 394054 . - 0 Rv0327c cyp135A1 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Possible cytochrome P450 135A1 Cyp135A1 Rv0327c, (MT0342, MTCY63.32c), len: 449 aa. Possible cyp135A1, cytochrome P450, similar to cytochrome P-450 monoxygenases and other cytochrome P-450 related enzymes e.g. FQ12609 putative P450 monooxygenase (506 aa), FASTA scores: opt: 276, E() : 1.7e-11, (27.9% identity in 433 aa overlap). Also similar to other Mycobacterium tuberculosis proteins e.g. MTV039.06|Rv0568 putative cytochrome P450 (472 aa); MTCI5.10 cytochrome p450 FASTA score: (30.4% identity in 434 aa overlap). Contains cytochrome P450 cysteine heme-iron ligand signature (PS00086). Belongs to the cytochrome P450 family. Alternative start possible at 33706 but no RBS. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O08447 No intermediary metabolism and respiration Mb0334c NC_000962.3 Mycobrowser_v2 RBS 394100 394103 . + 0 Rv0328 Rv0328 Possibly involved in a transcriptional mechanism. Possible transcriptional regulatory protein (possibly TetR/AcrR-family) Rv0328, (MTCY63.33), len: 200 aa. Possible transcription regulator, TetR/acrR family, similar in part to various hypothetical transcriptional regulators e.g. T36696|4726006|CAB41735.1|AL049731 probable regulatory protein from Streptomyces coelicolor (197 aa). Also some similarity with YX44_MYCTU|Q10829 hypothetical transcriptional regulator from Mycobacterium tuberculosis (195 aa), FASTA scores: opt: 154, E(): 0.00061, (26.7% identity in 202 aa overlap). Contains probable helix-turn helix motif from aa 27-48 (Score 1408, +3.98 SD). Seems to belong to the TetR/AcrR family of transcriptional regulators. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07252 No regulatory proteins Mb0335 NC_000962.3 Mycobrowser_v2 CDS 394111 394713 . + 0 Rv0328 Rv0328 Possibly involved in a transcriptional mechanism. Possible transcriptional regulatory protein (possibly TetR/AcrR-family) Rv0328, (MTCY63.33), len: 200 aa. Possible transcription regulator, TetR/acrR family, similar in part to various hypothetical transcriptional regulators e.g. T36696|4726006|CAB41735.1|AL049731 probable regulatory protein from Streptomyces coelicolor (197 aa). Also some similarity with YX44_MYCTU|Q10829 hypothetical transcriptional regulator from Mycobacterium tuberculosis (195 aa), FASTA scores: opt: 154, E(): 0.00061, (26.7% identity in 202 aa overlap). Contains probable helix-turn helix motif from aa 27-48 (Score 1408, +3.98 SD). Seems to belong to the TetR/AcrR family of transcriptional regulators. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07252 No regulatory proteins Mb0335 NC_000962.3 Mycobrowser_v2 CDS 394694 395320 . - 0 Rv0329c Rv0329c Function unknown Conserved hypothetical protein Rv0329c, (MTCY63.34c), len: 208 aa. Conserved hypothetical protein, showing some similarity with others hypothetical proteins and methyltransferases e.g. MitM|AF127374_14 methyltransferase from Streptomyces lavendulae (283 aa), FASTA scores: opt: 242, E(): 1.8e-08, (37.2% identity in 145 aa overlap); Q48938 from Methanosarcina barkeri (262 aa), FASTA scores: opt: 194, E(): 3.6e-06, (31.1% identity in 119 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07253 No conserved hypotheticals MSMEG_2632 Mb0336c NC_000962.3 Mycobrowser_v2 RBS 395329 395332 . - 0 Rv0329c Rv0329c Function unknown Conserved hypothetical protein Rv0329c, (MTCY63.34c), len: 208 aa. Conserved hypothetical protein, showing some similarity with others hypothetical proteins and methyltransferases e.g. MitM|AF127374_14 methyltransferase from Streptomyces lavendulae (283 aa), FASTA scores: opt: 242, E(): 1.8e-08, (37.2% identity in 145 aa overlap); Q48938 from Methanosarcina barkeri (262 aa), FASTA scores: opt: 194, E(): 3.6e-06, (31.1% identity in 119 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07253 No conserved hypotheticals MSMEG_2632 Mb0336c NC_000962.3 Mycobrowser_v2 CDS 395347 396087 . - 0 Rv0330c Rv0330c Unknown Hypothetical protein Rv0330c, (MTCY63.35c), len: 246 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07254 No conserved hypotheticals Mb0337c NC_000962.3 Mycobrowser_v2 RBS 396187 396191 . + 0 Rv0331 Rv0331 Function unknown; probably involved in cellular metabolism. Possible dehydrogenase/reductase Rv0331, (MTCY63.36), len: 388 aa. Possible dehydrogenase/reductase, similar to various dehydrogenases/reductases e.g. NP_103779.1|14022957|BAB49565.1|AP002999 flavoprotein reductase from Mesorhizobium loti (377 aa); NP_147681.1 predicted NAD(FAD)-dependent dehydrogenase from Aeropyrum pernix (381 aa); DHSU_CHRVI|Q06530 sulfide dehydrogenase (431 aa), FASTA scores: opt: 347, E(): 6.8e-15, (25.6% identity in 348 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07255 No intermediary metabolism and respiration Mb0338 NC_000962.3 Mycobrowser_v2 CDS 396201 397367 . + 0 Rv0331 Rv0331 Function unknown; probably involved in cellular metabolism. Possible dehydrogenase/reductase Rv0331, (MTCY63.36), len: 388 aa. Possible dehydrogenase/reductase, similar to various dehydrogenases/reductases e.g. NP_103779.1|14022957|BAB49565.1|AP002999 flavoprotein reductase from Mesorhizobium loti (377 aa); NP_147681.1 predicted NAD(FAD)-dependent dehydrogenase from Aeropyrum pernix (381 aa); DHSU_CHRVI|Q06530 sulfide dehydrogenase (431 aa), FASTA scores: opt: 347, E(): 6.8e-15, (25.6% identity in 348 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07255 No intermediary metabolism and respiration Mb0338 NC_000962.3 Mycobrowser_v2 CDS 398658 399524 . + 0 Rv0334 rmlA dTDP-L-rhamnose biosynthesis within the O antigen biosynthesis pathway of lipopolysaccharide biosynthesis [catalytic activity: dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-glucose]. Alpha-D-glucose-1-phosphate thymidylyltransferase RmlA (dTDP-glucose synthase) (dTDP-glucose pyrophosphorylase) Rv0334, (MTCY279.01), len: 288 aa. RmlA (alternate gene name: rfbA), alpha-D-glucose-1-phosphate thymidylyl-transferase (see citations below), equivalent to CAC32020.1|AL583925 glucose-1-phosphate thymidyltransferase from Mycobacterium leprae (288 aa). Also highly similar to others e.g. AAG29804.1|AF235050 glucose-1-phosphate thymidylyltransferase from Streptomyces rishiriensis (296 aa); RBA1_ECOLI|P37744 glucose-1-phosphate thymidylyltransferase from Escherichia coli strain K12 (293 aa), FASTA scores: opt: 1199, E(): 0, (62.0% identity in 284 aa overlap). Belongs to the glucose-1-phosphate thymidylyltransferase family. P72017 No intermediary metabolism and respiration ML2503 MMAR_0606 MSMEG_0384 Mb0341 NC_000962.3 Mycobrowser_v2 CDS 399535 400050 . - 0 Rv0335c PE6 Function unknown PE family protein PE6 Rv0335c, (MTCY279.02c), len: 171 aa. PE6, Member of the Mycobacterium tuberculosis PE family (see Brennan & Delogu 2002); contains short region of similarity to part of the unique N-terminus of the Mycobacterium tuberculosis PGRS family of Glycine-rich proteins e.g. Y03A_MYCTU|Q10637 hypothetical glycine-rich 49.6 kd protein (603 aa), FASTA scores: opt: 219, E(): 1.1e-08, (51.5% identity in 66 aa overlap). O86338 No PE/PPE Mb0342c NC_000962.3 Mycobrowser_v2 CDS 443067 444197 . - 0 Rv0365c Rv0365c Function unknown: may be involved in the ability to survive in macrophages. Conserved protein Rv0365c, (MTCY13E10.27c), len: 376 aa (start uncertain). Conserved protein (see citation below), very similar to G388212|CAA35191.1, a truncated ORF immediately upstream of the Corynebacterium glutamicum fda gene encoding fructose-1,6-biphosphate aldolase (304 aa), FASTA scores: E(): 7.1e-19, (42.2% identity in 296 aa overlap). O06315 No conserved hypotheticals MMAR_0667 MSMEG_0740 Mb0372c NC_000962.3 Mycobrowser_v2 RBS 400184 400188 . + 0 Rv0336 Rv0336 Function unknown Conserved 13E12 repeat family protein Rv0336, (MTCY279.03), len: 503 aa. Part of Mycobacterium tuberculosis 13E12 repeat family; almost identical to Rv0515|MTCY20G10.05 hypothetical protein from Mycobacterium tuberculosis FASTA scores: (99.8% identity in 503 aa overlap), possibly due to a recent gene duplication. Also similar to other Mycobacterium tuberculosis hypothetical proteins e.g. Rv1148c, Rv1945, etc. O33266 No insertion seqs and phages MMAR_5187 MSMEG_5026 Mb0343 NC_000962.3 Mycobrowser_v2 CDS 400192 401703 . + 0 Rv0336 Rv0336 Function unknown Conserved 13E12 repeat family protein Rv0336, (MTCY279.03), len: 503 aa. Part of Mycobacterium tuberculosis 13E12 repeat family; almost identical to Rv0515|MTCY20G10.05 hypothetical protein from Mycobacterium tuberculosis FASTA scores: (99.8% identity in 503 aa overlap), possibly due to a recent gene duplication. Also similar to other Mycobacterium tuberculosis hypothetical proteins e.g. Rv1148c, Rv1945, etc. O33266 No insertion seqs and phages MMAR_5187 MSMEG_5026 Mb0343 NC_000962.3 Mycobrowser_v2 CDS 401873 403162 . - 0 Rv0337c aspC Generates oxaloacetate and L-glutamate from L-aspartate and 2-oxoglutarate [catalytic activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate]. Probable aspartate aminotransferase AspC (transaminase A) (ASPAT) Rv0337c, (MTCY279.04c), len: 429 aa. Probable aspC, aspartate aminotransferase (transaminase A), equivalent to CAC32019.1|AL583925 probable aspartate aminotransferase from Mycobacterium leprae (437 aa). Also highly similar to many e.g. Q48143|U32823 aspartate aminotransferase (404 aa), FASTA scores: opt: 1646, E(): 0, (57.2% identity in 404 aa overlap). Also some similarity to Rv3565|MTCY06G11.12 from Mycobacterium tuberculosis FASTA score: (27.2% identity in 383 aa overlap). Belongs to class-I of pyridoxal-phosphate-dependent aminotransferases. Cofactor: pyridoxal phosphate. P63498 No intermediary metabolism and respiration ML2502 MMAR_0610 MSMEG_0688 Mb0344c NC_000962.3 Mycobrowser_v2 CDS 2139076 2139687 . - 0 Rv1890c Rv1890c Unknown Hypothetical protein Rv1890c, (MTCY180.28), len: 203 aa. Hypothetical unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). O07742 No conserved hypotheticals ML2026 MMAR_2781 MSMEG_0101 Mb1923c NC_000962.3 Mycobrowser_v2 CDS 417304 418290 . + 0 Rv0347 Rv0347 Unknown Probable conserved membrane protein Rv0347, (MTCY13E10.07), len: 328 aa (alternative start possible). Probable conserved membrane protein, similar to Rv0831c|AL022004|MTV043_23 from Mycobacterium tuberculosis (271 aa), FASTA scores: E(): 9.6e-21, (33.1% identity in 266 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06298 No cell wall and cell processes Mb0355 NC_000962.3 Mycobrowser_v2 RBS 418283 418287 . + 0 Rv0347 Rv0347 Unknown Probable conserved membrane protein Rv0347, (MTCY13E10.07), len: 328 aa (alternative start possible). Probable conserved membrane protein, similar to Rv0831c|AL022004|MTV043_23 from Mycobacterium tuberculosis (271 aa), FASTA scores: E(): 9.6e-21, (33.1% identity in 266 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06298 No cell wall and cell processes Mb0355 NC_000962.3 Mycobrowser_v2 CDS 403193 405841 . - 0 Rv0338c Rv0338c Function unknown; probably involved in cellular metabolism. Probable iron-sulfur-binding reductase Rv0338c, (MTCY279.05c), len: 882 aa. Probable iron-sulphur-binding reductase, possibly membrane-bound, equivalent to CAC32018.1|AL583925 probable iron-sulphur-binding reductase from Mycobacterium leprae (880 aa). Also highly similar to others e.g. T36608|5019323|CAB44376.1|AL078610 probable iron-sulfur-binding reductase from Streptomyces coelicolor (760 aa), FASTA scores: opt: 1658, E(): 0, (49.9% identity in 772 aa overlap); BAB07521.1|AP001520 iron-sulphur-binding reductase from Bacillus halodurans (700 aa). Contains PS00070 Aldehyde dehydrogenases cysteine active site and two of PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature. First of several possible start sites chosen. O33268 No intermediary metabolism and respiration ML2501 MMAR_0611 MSMEG_0690 Mb0345c NC_000962.3 Mycobrowser_v2 CDS 405950 408448 . - 0 Rv0339c Rv0339c Could be involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0339c, (MTCY279.06c), len: 832 aa. Possible transcriptional regulator, showing very weak similarity with parts of others. Contains PS00017 ATP/GTP-binding site motif A (P-loop); and probable helix-turn helix motif from aa 778-799 (Score 1041, +2.73 SD). O33269 No regulatory proteins MMAR_0612 MSMEG_0691 Mb0346c NC_000962.3 Mycobrowser_v2 RBS 408621 408627 . + 0 Rv0340 Rv0340 Function unknown Conserved protein Rv0340, (MTCY279.07), len: 179 aa. Conserved protein; MEME-mast analysis shows similarity to product of downstream gene, Rv0341|iniB. O33270 No conserved hypotheticals MMAR_0613 Mb0347 NC_000962.3 Mycobrowser_v2 CDS 408634 409173 . + 0 Rv0340 Rv0340 Function unknown Conserved protein Rv0340, (MTCY279.07), len: 179 aa. Conserved protein; MEME-mast analysis shows similarity to product of downstream gene, Rv0341|iniB. O33270 No conserved hypotheticals MMAR_0613 Mb0347 NC_000962.3 Mycobrowser_v2 RBS 409349 409352 . + 0 Rv0341 iniB Unknown Isoniazid inductible gene protein IniB Rv0341, (MTCY13E10.01), len: 479 aa. IniB, isoniazid-inducible gene, (see citations below). Protein very Gly-, Ala-rich, similar to cell wall proteins e.g. P27483|GRP_ARATH glycine-rich cell wall structural protein from A.thaliana (338 aa), FASTA scores: opt: 532, E(): 5.2e-13, (39.3% identity in 321 aa overlap). MEME-mast analysis shows similarity to product of upstream gene, Rv0340. O06292 No cell wall and cell processes Mb0348 NC_000962.3 Mycobrowser_v2 CDS 409362 410801 . + 0 Rv0341 iniB Unknown Isoniazid inductible gene protein IniB Rv0341, (MTCY13E10.01), len: 479 aa. IniB, isoniazid-inducible gene, (see citations below). Protein very Gly-, Ala-rich, similar to cell wall proteins e.g. P27483|GRP_ARATH glycine-rich cell wall structural protein from A.thaliana (338 aa), FASTA scores: opt: 532, E(): 5.2e-13, (39.3% identity in 321 aa overlap). MEME-mast analysis shows similarity to product of upstream gene, Rv0340. O06292 No cell wall and cell processes Mb0348 NC_000962.3 Mycobrowser_v2 CDS 444222 444815 . - 0 Rv0366c Rv0366c Function unknown Conserved hypothetical protein Rv0366c, (MTV036.01c), len: 197 aa. Conserved hypothetical protein, showing weak similarity to HI1395|P44173|YD95_HAEIN hypothetical protein from Haemophilus influenzae (140 aa), FASTA scores: opt: 152, E(): 0.0015, (27.0% identity in 126 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS00850 Glycine radical signature. O53701 No conserved hypotheticals MSMEG_3436 Mb0373c NC_000962.3 Mycobrowser_v2 CDS 3131770 3132714 . - 0 Rv2824c Rv2824c Unknown Hypothetical protein Rv2824c, (MTCY16B7.18), len: 314 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P71628 No conserved hypotheticals Mb2848c NC_000962.3 Mycobrowser_v2 RBS 410825 410829 . + 0 Rv0342 iniA Unknown Isoniazid inductible gene protein IniA Rv0342, iniA, (MTCY13E10.02), len: 640 aa. IniA, isoniazid-inducible gene, (see citations below). Shows slight similarity to some hypothetical bacterial proteins e.g. P40983|YOR6_THER hypothetical protein (402 aa), FASTA scores: opt: 242, E(): 1.4e-07, (22.3% identity in 349 aa overlap). Also some similarity to downstream ORF Rv0343|iniC. Possible transmembrane stretch around residue 490. Alternative start site exists at 410824. Contains a phosphopantetheine attachment site motif suggestive of an acyl carrier protein. Note that the iniA gene is also induced by the antibiotic ethambutol, an agent that inhibits cell wall biosynthesis by a mechanism that is distinct from isoniazid. O06293 No cell wall and cell processes MMAR_0615 MSMEG_0695 Mb0349 NC_000962.3 Mycobrowser_v2 CDS 410838 412760 . + 0 Rv0342 iniA Unknown Isoniazid inductible gene protein IniA Rv0342, iniA, (MTCY13E10.02), len: 640 aa. IniA, isoniazid-inducible gene, (see citations below). Shows slight similarity to some hypothetical bacterial proteins e.g. P40983|YOR6_THER hypothetical protein (402 aa), FASTA scores: opt: 242, E(): 1.4e-07, (22.3% identity in 349 aa overlap). Also some similarity to downstream ORF Rv0343|iniC. Possible transmembrane stretch around residue 490. Alternative start site exists at 410824. Contains a phosphopantetheine attachment site motif suggestive of an acyl carrier protein. Note that the iniA gene is also induced by the antibiotic ethambutol, an agent that inhibits cell wall biosynthesis by a mechanism that is distinct from isoniazid. O06293 No cell wall and cell processes MMAR_0615 MSMEG_0695 Mb0349 NC_000962.3 Mycobrowser_v2 RBS 421700 421704 . + 0 Rv0351 grpE Stimulates, jointly with DNAJ|Rv0352, the ATPase activity of DNAK|Rv0350. HELPS to release ADP from DNAK thus allowing DNAK to recycle more efficiently. Seems to be regulated negatively by HSPR (Rv0353 product). Probable GrpE protein (HSP-70 cofactor) Rv0351, (MTCY13E10.11), len: 235 aa. Probable grpE protein (HSP-70 cofactor), equivalent to CAC32012.1|AL583925 Hsp70 cofactor from Mycobacterium leprae (229 aa). Also highly similar to others eg Q05562|GRPE_STRCO|2127521|PN0643 GRPE protein from Streptomyces coelicolor (225 aa). Contains grpE protein signature (PS01071). Belongs to the GrpE family. P32724 No virulence, detoxification, adaptation ML2495 MMAR_0638 MSMEG_0710 Mb0359 NC_000962.3 Mycobrowser_v2 CDS 421709 422416 . + 0 Rv0351 grpE Stimulates, jointly with DNAJ|Rv0352, the ATPase activity of DNAK|Rv0350. HELPS to release ADP from DNAK thus allowing DNAK to recycle more efficiently. Seems to be regulated negatively by HSPR (Rv0353 product). Probable GrpE protein (HSP-70 cofactor) Rv0351, (MTCY13E10.11), len: 235 aa. Probable grpE protein (HSP-70 cofactor), equivalent to CAC32012.1|AL583925 Hsp70 cofactor from Mycobacterium leprae (229 aa). Also highly similar to others eg Q05562|GRPE_STRCO|2127521|PN0643 GRPE protein from Streptomyces coelicolor (225 aa). Contains grpE protein signature (PS01071). Belongs to the GrpE family. P32724 No virulence, detoxification, adaptation ML2495 MMAR_0638 MSMEG_0710 Mb0359 NC_000962.3 Mycobrowser_v2 CDS 412757 414238 . + 0 Rv0343 iniC Unknown Isoniazid inductible gene protein IniC Rv0343, (MTCY13E10.03), len: 493 aa. IniC, isoniazid-inducible gene, (see citations below). Shows slight similarity to P40983|YOR6_THER8 hypothetical protein (402 aa), FASTA scores: opt: 196, E(): 2.6e-05, (25.9% identity in 228 aa overlap). Also some similarity to upstream ORF Rv0342|iniA. Contains (PS00017) ATP/GTP-binding site motif A (P-loop). Note that the iniA gene is also induced by the antibiotic ethambutol, an agent that inhibits cell wall biosynthesis by a mechanism that is distinct from isoniazid. O06294 No cell wall and cell processes MMAR_0616 MSMEG_0698 Mb0350 NC_000962.3 Mycobrowser_v2 CDS 414381 414941 . - 0 Rv0344c lpqJ Unknown Probable lipoprotein LpqJ Rv0344c, (MTCY13E10.04c), len: 186 aa. Probable lipoprotein, without homology. Has an appropriately positioned prokaryotic lipoprotein signature (PS00013). O06295 No cell wall and cell processes MMAR_0620 MSMEG_0704 Mb0351c NC_000962.3 Mycobrowser_v2 RBS 414948 414951 . - 0 Rv0344c lpqJ Unknown Probable lipoprotein LpqJ Rv0344c, (MTCY13E10.04c), len: 186 aa. Probable lipoprotein, without homology. Has an appropriately positioned prokaryotic lipoprotein signature (PS00013). O06295 No cell wall and cell processes MMAR_0620 MSMEG_0704 Mb0351c NC_000962.3 Mycobrowser_v2 RBS 415039 415042 . + 0 Rv0345 Rv0345 Function unknown Conserved hypothetical protein Rv0345, (MTCY13E10.05), len: 136 aa. Conserved hypothetical protein, similar to other hypothetical proteins e.g. AL13282 4|SCAH10_9 hypothetical protein from Streptomyces coelicolor (207 aa), FASTA scores: opt: 188, E(): 1.5e-05, (41.0% identity in 117 aa overlap). O06296 No conserved hypotheticals MMAR_0624 MSMEG_1806 Mb0352,Mb0353 NC_000962.3 Mycobrowser_v2 CDS 415050 415460 . + 0 Rv0345 Rv0345 Function unknown Conserved hypothetical protein Rv0345, (MTCY13E10.05), len: 136 aa. Conserved hypothetical protein, similar to other hypothetical proteins e.g. AL13282 4|SCAH10_9 hypothetical protein from Streptomyces coelicolor (207 aa), FASTA scores: opt: 188, E(): 1.5e-05, (41.0% identity in 117 aa overlap). O06296 No conserved hypotheticals MMAR_0624 MSMEG_1806 Mb0352,Mb0353 NC_000962.3 Mycobrowser_v2 CDS 415502 416965 . - 0 Rv0346c ansP2 Thought to be involved in transport of L-asparagine across the membrane. Responsible for the translocation of the substrate across the membrane. Possible L-asparagine permease AnsP2 (L-asparagine transport protein) Rv0346c, (MTCY13E10.06c), len: 487 aa. Possible ansP2, L-asparagine permease, integral membrane protein belonging to family containing many amino acid permeases, highly similar to G467030|B2126_F2_85|NP_301937.1|NC_002677 probable L-asparagine permease from Mycobacterium leprae (498 aa); and NP_301938.1|NC_002677 probable L-asparagine permease from Mycobacterium leprae (505 aa). Also highly similar to others e.g. P77610|ANSP_ECOLI L-asparagine permease from Escherichia coli strain K-12 (499 aa). Also highly similar to ANSP1|Rv2127|MT2186|MTCY261_22|O33261 probable L-asparagine permease from Mycobacterium tuberculosis (489 aa), FASTA score: (72.1% identity in 473 aa overlap). And shows some similarity to MTCY3G12.14 from Mycobacterium tuberculosis. Belongs to the amino acid permease family (APC family). Note that previously known as aroP2. P0A4W0 No cell wall and cell processes ML1304,ML1305 MMAR_0627 Mb0354c NC_000962.3 Mycobrowser_v2 RBS 416970 416973 . - 0 Rv0346c ansP2 Thought to be involved in transport of L-asparagine across the membrane. Responsible for the translocation of the substrate across the membrane. Possible L-asparagine permease AnsP2 (L-asparagine transport protein) Rv0346c, (MTCY13E10.06c), len: 487 aa. Possible ansP2, L-asparagine permease, integral membrane protein belonging to family containing many amino acid permeases, highly similar to G467030|B2126_F2_85|NP_301937.1|NC_002677 probable L-asparagine permease from Mycobacterium leprae (498 aa); and NP_301938.1|NC_002677 probable L-asparagine permease from Mycobacterium leprae (505 aa). Also highly similar to others e.g. P77610|ANSP_ECOLI L-asparagine permease from Escherichia coli strain K-12 (499 aa). Also highly similar to ANSP1|Rv2127|MT2186|MTCY261_22|O33261 probable L-asparagine permease from Mycobacterium tuberculosis (489 aa), FASTA score: (72.1% identity in 473 aa overlap). And shows some similarity to MTCY3G12.14 from Mycobacterium tuberculosis. Belongs to the amino acid permease family (APC family). Note that previously known as aroP2. P0A4W0 No cell wall and cell processes ML1304,ML1305 MMAR_0627 Mb0354c NC_000962.3 Mycobrowser_v2 CDS 418293 418946 . + 0 Rv0348 Rv0348 Could be involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0348, (MTCY13E10.08), len: 217 aa. Possible transcriptional regulator, showing some similarity to O53334|RV3188|MTV014.32 conserved hypothetical protein from Mycobacterium tuberculosis (115 aa), FASTA score: (30.0% identity in 100 aa overlap). Contains probable helix-turn helix motif from aa 89-110 (Score 1407, +3.98 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06299 No regulatory proteins Mb0356 NC_000962.3 Mycobrowser_v2 CDS 418949 419608 . + 0 Rv0349 Rv0349 Unknown Hypothetical protein Rv0349, (MTCY13E10.09), len: 219 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06300 No conserved hypotheticals Mb0357 NC_000962.3 Mycobrowser_v2 RBS 419822 419827 . + 0 Rv0350 dnaK Acts as a chaperone. Involved in induction by stress conditions e.g. heat shock. Possibly has an ATPase activity. Seems to be regulated positively by sigh (Rv3223c product) and negatively by HSPR (Rv0353 product). Probable chaperone protein DnaK (heat shock protein 70) (heat shock 70 kDa protein) (HSP70) Rv0350, (MTCY13E10.10), len: 625 aa. Probable dnaK (alternate gene name: hsp70), 70 kDa heat shock protein (see citations below), equivalent to AAA25362.1|M95576|1924344A|738248 heat shock protein 70 from Mycobacterium leprae (621 aa); and DNAK_MYCPA|Q00488 (623 aa), FASTA scores: opt: 3678, E(): 0, (92.3% identity in 625 aa overlap). Also highly similar to others e.g. Q05558|DNAK_STRCO|453231|CAA54606.1|X77458 chaperone protein DNAK from Streptomyces coelicolor (618 aa). Has probably an ATPase activity. Note that this sequence differs from DNAK_MYCTU|P32723 (609 aa), due to a frameshift near the N-terminus. Belongs to the heat shock protein 70 family. P0A5B9 No virulence, detoxification, adaptation ML2496 MMAR_0637 MSMEG_0709 Mb0358 NC_000962.3 Mycobrowser_v2 CDS 419835 421712 . + 0 Rv0350 dnaK Acts as a chaperone. Involved in induction by stress conditions e.g. heat shock. Possibly has an ATPase activity. Seems to be regulated positively by sigh (Rv3223c product) and negatively by HSPR (Rv0353 product). Probable chaperone protein DnaK (heat shock protein 70) (heat shock 70 kDa protein) (HSP70) Rv0350, (MTCY13E10.10), len: 625 aa. Probable dnaK (alternate gene name: hsp70), 70 kDa heat shock protein (see citations below), equivalent to AAA25362.1|M95576|1924344A|738248 heat shock protein 70 from Mycobacterium leprae (621 aa); and DNAK_MYCPA|Q00488 (623 aa), FASTA scores: opt: 3678, E(): 0, (92.3% identity in 625 aa overlap). Also highly similar to others e.g. Q05558|DNAK_STRCO|453231|CAA54606.1|X77458 chaperone protein DNAK from Streptomyces coelicolor (618 aa). Has probably an ATPase activity. Note that this sequence differs from DNAK_MYCTU|P32723 (609 aa), due to a frameshift near the N-terminus. Belongs to the heat shock protein 70 family. P0A5B9 No virulence, detoxification, adaptation ML2496 MMAR_0637 MSMEG_0709 Mb0358 NC_000962.3 Mycobrowser_v2 RBS 422436 422439 . + 0 Rv0352 dnaJ1 Acts as a co-chaperone. Stimulates, jointly with GRPE|Rv0351, the ATPase activity of DNAK|Rv0350. Seems to be regulated negatively by HSPR (Rv0353 product). Probable chaperone protein DnaJ1 Rv0352, (MTCY13E10.12), len: 395 aa. Probable dnaJ1, chaperone protein, equivalent to AAA25363.1|M95576 DNA J heatshock protein from Mycobacterium leprae (389 aa). Also highly similar to others. Contains both DnaJ signatures (PS00636, and PS00637). Belongs to the DNAJ family. Cofactor: binds two zinc ions per monomer. Note that sequence differs from DNAJ_MYCTU|P07881 due to a frameshift at the N-terminus. Note that previously known as dnaJ. P0A548 No virulence, detoxification, adaptation ML2494 MMAR_0639 MSMEG_0711 Mb0360 NC_000962.3 Mycobrowser_v2 CDS 422452 423639 . + 0 Rv0352 dnaJ1 Acts as a co-chaperone. Stimulates, jointly with GRPE|Rv0351, the ATPase activity of DNAK|Rv0350. Seems to be regulated negatively by HSPR (Rv0353 product). Probable chaperone protein DnaJ1 Rv0352, (MTCY13E10.12), len: 395 aa. Probable dnaJ1, chaperone protein, equivalent to AAA25363.1|M95576 DNA J heatshock protein from Mycobacterium leprae (389 aa). Also highly similar to others. Contains both DnaJ signatures (PS00636, and PS00637). Belongs to the DNAJ family. Cofactor: binds two zinc ions per monomer. Note that sequence differs from DNAJ_MYCTU|P07881 due to a frameshift at the N-terminus. Note that previously known as dnaJ. P0A548 No virulence, detoxification, adaptation ML2494 MMAR_0639 MSMEG_0711 Mb0360 NC_000962.3 Mycobrowser_v2 RBS 423625 423629 . + 0 Rv0353 hspR Involved in transcriptional regulation (repression) of heat shock proteins e.g. DNAK|Rv0350, GRPE|Rv0351, DNAJ1|Rv0352. Binds to three inverted repeats (IR1-IR3) in the promoter region of the DNAK operon. Induction: by heat shock. Probable heat shock protein transcriptional repressor HspR (MerR family) Rv0353, (MTCY13E10.13), len: 126 aa. Probable hspR, heat shock regulatory protein (see Stewart et al., 2001), merR family, highly similar to others e.g. HspR|P40183 heat shock regulatory protein from Streptomyces coelicolor (151 aa), FASTA scores: E(): 4.9e-22, (55.7% identity in 140 aa overlap), that binds to three inverted repeats (IR1-IR3) in the promoter region of the dnaK operon. Has possible coiled coil region in C-terminal half. Belongs to the MerR family of transcriptional regulators. O06302 No virulence, detoxification, adaptation ML2493 MMAR_0640 MSMEG_0713 Mb0361 NC_000962.3 Mycobrowser_v2 CDS 423639 424019 . + 0 Rv0353 hspR Involved in transcriptional regulation (repression) of heat shock proteins e.g. DNAK|Rv0350, GRPE|Rv0351, DNAJ1|Rv0352. Binds to three inverted repeats (IR1-IR3) in the promoter region of the DNAK operon. Induction: by heat shock. Probable heat shock protein transcriptional repressor HspR (MerR family) Rv0353, (MTCY13E10.13), len: 126 aa. Probable hspR, heat shock regulatory protein (see Stewart et al., 2001), merR family, highly similar to others e.g. HspR|P40183 heat shock regulatory protein from Streptomyces coelicolor (151 aa), FASTA scores: E(): 4.9e-22, (55.7% identity in 140 aa overlap), that binds to three inverted repeats (IR1-IR3) in the promoter region of the dnaK operon. Has possible coiled coil region in C-terminal half. Belongs to the MerR family of transcriptional regulators. O06302 No virulence, detoxification, adaptation ML2493 MMAR_0640 MSMEG_0713 Mb0361 NC_000962.3 Mycobrowser_v2 CDS 424777 434679 . - 0 Rv0355c PPE8 Function unknown PPE family protein PPE8 Rv0355c, (MTCY13E10.15c, MTCY13E10.16c, MTCY13E10.17c), len: 3300 aa. PPE8, Member of the Mycobacterium tuberculosis PPE family, similar to others e.g. AL009198|MTV004_5 from Mycobacterium tuberculosis (3716 aa), FASTA scores: opt: 2906, E(): 0, (40.9% identity in 3833 aa overlap); MTV004_3 FASTA scores: (39.0% identity in 3531 aa overlap); etc. Gene contains large number of clustered Major Polymorphic Tandem Repeats (MPTR). Related to MTCY13E10.16c, E(): 0; MTCY13E10.17c, E(): 0; MTCY48.17, E(): 0; MTCY98.0034c, E(): 0; MTCY03C7.23 E(): 0; MTCY98.0031c, E(): 0; MTCY31.06c, E(): 5.6e-17; MTCY359.33, E(): 2.3e-16. Nucleotide position 426909 in the genome sequence has been corrected, A:C resulting in W2591G. Q6MX44 No PE/PPE MMAR_0642 Mb0362c NC_000962.3 Mycobrowser_v2 CDS 434830 435474 . - 0 Rv0356c Rv0356c Function unknown Conserved protein Rv0356c, (MTCY13E10.18c), len: 214 aa. Conserved protein, equivalent to AL023514|MLCB4_12 conserved hypothetical protein from Mycobacterium leprae (218 aa), FASTA scores: opt: 1067, E(): 0, (73.4% identity in 214 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06307 No conserved hypotheticals ML0279 MMAR_0681 MSMEG_0760 Mb0363c NC_000962.3 Mycobrowser_v2 RBS 435481 435484 . - 0 Rv0356c Rv0356c Function unknown Conserved protein Rv0356c, (MTCY13E10.18c), len: 214 aa. Conserved protein, equivalent to AL023514|MLCB4_12 conserved hypothetical protein from Mycobacterium leprae (218 aa), FASTA scores: opt: 1067, E(): 0, (73.4% identity in 214 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06307 No conserved hypotheticals ML0279 MMAR_0681 MSMEG_0760 Mb0363c NC_000962.3 Mycobrowser_v2 CDS 435471 436769 . - 0 Rv0357c purA Involved in AMP biosynthesis (first committed step). Plays an important role in the de novo pathway of purine nucleotide biosynthesis [catalytic activity: GTP + imp + L-aspartate = GDP + phosphate + adenylosuccinate]. Probable adenylosuccinate synthetase PurA (imp--aspartate ligase) (ADSS) (ampsase) Rv0357c, (MTCY13E10.19c), len: 432 aa. Probable purA, adenylosuccinate synthase, equivalent to AL023514|MLCB4_13 from adenylosuccinate synthetase Mycobacterium leprae (432 aa), FASTA scores: opt: 2555, E(): 0, (87.9% identity in 431 aa overlap). Also highly similar to many bacterial adenylosuccinates synthetases e.g. P12283|PURA_ECOLI adenylosuccinates synthetase from Escherichia coli (431 aa), FASTA scores: E(): 0, (51.1% identity in 425 aa overlap); etc. Belongs to the adenylosuccinate synthetase family. P65880 No intermediary metabolism and respiration ML0280 MMAR_0680 MSMEG_0759 Mb0364c NC_000962.3 Mycobrowser_v2 RBS 436776 436779 . - 0 Rv0357c purA Involved in AMP biosynthesis (first committed step). Plays an important role in the de novo pathway of purine nucleotide biosynthesis [catalytic activity: GTP + imp + L-aspartate = GDP + phosphate + adenylosuccinate]. Probable adenylosuccinate synthetase PurA (imp--aspartate ligase) (ADSS) (ampsase) Rv0357c, (MTCY13E10.19c), len: 432 aa. Probable purA, adenylosuccinate synthase, equivalent to AL023514|MLCB4_13 from adenylosuccinate synthetase Mycobacterium leprae (432 aa), FASTA scores: opt: 2555, E(): 0, (87.9% identity in 431 aa overlap). Also highly similar to many bacterial adenylosuccinates synthetases e.g. P12283|PURA_ECOLI adenylosuccinates synthetase from Escherichia coli (431 aa), FASTA scores: E(): 0, (51.1% identity in 425 aa overlap); etc. Belongs to the adenylosuccinate synthetase family. P65880 No intermediary metabolism and respiration ML0280 MMAR_0680 MSMEG_0759 Mb0364c NC_000962.3 Mycobrowser_v2 RBS 436849 436852 . + 0 Rv0358 Rv0358 Function unknown Conserved protein Rv0358, (MTCY13E10.20), len: 215 aa. Conserved protein, highly similar to ML0281|AL023514|MLCB4_14 conserved hypothetical protein from Mycobacterium leprae (229 aa), FASTA scores: opt: 852, E(): 0, (62.9% identity in 229 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06308 No conserved hypotheticals ML0281 MMAR_0679 MSMEG_0758 Mb0365 NC_000962.3 Mycobrowser_v2 CDS 436860 437507 . + 0 Rv0358 Rv0358 Function unknown Conserved protein Rv0358, (MTCY13E10.20), len: 215 aa. Conserved protein, highly similar to ML0281|AL023514|MLCB4_14 conserved hypothetical protein from Mycobacterium leprae (229 aa), FASTA scores: opt: 852, E(): 0, (62.9% identity in 229 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06308 No conserved hypotheticals ML0281 MMAR_0679 MSMEG_0758 Mb0365 NC_000962.3 Mycobrowser_v2 CDS 437518 438297 . + 0 Rv0359 Rv0359 Unknown Probable conserved integral membrane protein Rv0359, (MTCY13E10.21), len: 259 aa. Probable conserved integral membrane protein, highly similar to hypothetical or other membrane proteins e.g. AL133220|SCC75A_6|T50569 probable membrane protein from Streptomyces coelicolor (265 aa), FASTA scores: opt: 642, E(): 0, (43.1% identity in 248 aa overlap); P70995 hypothetical 24.7 kDa protein from Bacillus subtilis (219 aa), FASTA scores: E(): 1.5e-12, (31.3% identity in 192 aa overlap). Contains neutral zinc metallopeptidases, zinc-binding region signature (PS00142). O06309 No cell wall and cell processes ML0282 MMAR_0678 MSMEG_0756 Mb0366 NC_000962.3 Mycobrowser_v2 RBS 438805 438809 . + 0 Rv0361 Rv0361 Unknown Probable conserved membrane protein Rv0361, (MTCY13E10.23), len: 275 aa. Probable conserved membrane protein (has hydrophobic stretch from residues 132-156), equivalent to AL023514|MLCB4_17|AA18949.1|AL023514 putative membrane protein from Mycobacterium leprae (292 aa), FASTA scores: opt: 1044, E(): 0, (58.6% identity in 292 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06311 No cell wall and cell processes ML0285 MMAR_0671 MSMEG_0753 Mb0368 NC_000962.3 Mycobrowser_v2 CDS 438822 439649 . + 0 Rv0361 Rv0361 Unknown Probable conserved membrane protein Rv0361, (MTCY13E10.23), len: 275 aa. Probable conserved membrane protein (has hydrophobic stretch from residues 132-156), equivalent to AL023514|MLCB4_17|AA18949.1|AL023514 putative membrane protein from Mycobacterium leprae (292 aa), FASTA scores: opt: 1044, E(): 0, (58.6% identity in 292 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06311 No cell wall and cell processes ML0285 MMAR_0671 MSMEG_0753 Mb0368 NC_000962.3 Mycobrowser_v2 RBS 439853 439856 . + 0 Rv0362 mgtE Thought to be involved in Mg2+ transport. Responsible for the translocation of the substrate across the membrane. Possible Mg2+ transport transmembrane protein MgtE Rv0362, (MTCY13E10.24), len: 460 aa. Possible mgtE, magnesium (Mg2+) transport transmembrane protein; C-terminal region is highly similar to MGTE|G780283 putative Mg2+ transporter from Providencia stuarti (314 aa), FASTA scores: E(): 0, (47.2% identity in 307 aa overlap) (N-terminus extends approx. 150 aa further upstream compared to P. stuarti ORF). Also similar in part to others e.g. AAK20879.1|AF334760_1|AF334760 putative Mg2+ transporter from Aeromonas hydrophila (455 aa); NP_231292.1|NC_002505 magnesium transporter from Vibrio cholerae (451 aa); NP_102305.1|NC_002678 Mg2+ transport protein from Mesorhizobium loti (454 aa); etc. Also similar to Rv1232c|MTV006.04c from Mycobacterium tuberculosis (435 aa). Extended hydrophobic segment spanning last 130 residues. Belongs to the MgtE family. O06312 No cell wall and cell processes MMAR_0670 MSMEG_6269 Mb0369 NC_000962.3 Mycobrowser_v2 CDS 439871 441253 . + 0 Rv0362 mgtE Thought to be involved in Mg2+ transport. Responsible for the translocation of the substrate across the membrane. Possible Mg2+ transport transmembrane protein MgtE Rv0362, (MTCY13E10.24), len: 460 aa. Possible mgtE, magnesium (Mg2+) transport transmembrane protein; C-terminal region is highly similar to MGTE|G780283 putative Mg2+ transporter from Providencia stuarti (314 aa), FASTA scores: E(): 0, (47.2% identity in 307 aa overlap) (N-terminus extends approx. 150 aa further upstream compared to P. stuarti ORF). Also similar in part to others e.g. AAK20879.1|AF334760_1|AF334760 putative Mg2+ transporter from Aeromonas hydrophila (455 aa); NP_231292.1|NC_002505 magnesium transporter from Vibrio cholerae (451 aa); NP_102305.1|NC_002678 Mg2+ transport protein from Mesorhizobium loti (454 aa); etc. Also similar to Rv1232c|MTV006.04c from Mycobacterium tuberculosis (435 aa). Extended hydrophobic segment spanning last 130 residues. Belongs to the MgtE family. O06312 No cell wall and cell processes MMAR_0670 MSMEG_6269 Mb0369 NC_000962.3 Mycobrowser_v2 CDS 441265 442299 . - 0 Rv0363c fba Involved in glycolysis (at the sixth step) [catalytic activity: D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate]. Probable fructose-bisphosphate aldolase Fba Rv0363c, (MTCY13E10.25c), len: 344 aa. Probable fba (alternate gene name: fda), fructose bisphosphate aldolase , equivalent to AL023514|MLCB4_18|O69600|ALF_MYCLE fructose-bisphosphate aldolase from Mycobacterium leprae (345 aa), FASTA scores: opt: 1995, E(): 0, (87.7% identity in 342 aa overlap). Also highly similar to others. Belongs to class II fructose-bisphosphate aldolase family. Cofactor: zinc. P67475 No intermediary metabolism and respiration ML0286 MMAR_0669 MSMEG_0752 Mb0370c NC_000962.3 Mycobrowser_v2 RBS 442308 442313 . - 0 Rv0363c fba Involved in glycolysis (at the sixth step) [catalytic activity: D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate]. Probable fructose-bisphosphate aldolase Fba Rv0363c, (MTCY13E10.25c), len: 344 aa. Probable fba (alternate gene name: fda), fructose bisphosphate aldolase , equivalent to AL023514|MLCB4_18|O69600|ALF_MYCLE fructose-bisphosphate aldolase from Mycobacterium leprae (345 aa), FASTA scores: opt: 1995, E(): 0, (87.7% identity in 342 aa overlap). Also highly similar to others. Belongs to class II fructose-bisphosphate aldolase family. Cofactor: zinc. P67475 No intermediary metabolism and respiration ML0286 MMAR_0669 MSMEG_0752 Mb0370c NC_000962.3 Mycobrowser_v2 CDS 476394 476642 . + 0 Rv0397A Rv0397A Unknown Conserved protein Rv0397A, len: 82 aa. Conserved protein. No conserved hypotheticals NC_000962.3 Mycobrowser_v2 CDS 445314 446525 . - 0 Rv0368c Rv0368c Function unknown Conserved hypothetical protein Rv0368c, (MTV036.03c), len: 403 aa. Conserved hypothetical protein, showing some similarity to AJ224684|BJAJ4684_4 cooxS protein from Bradyrhizobium japonicum (422 aa), FASTA scores: opt: 341, E(): 4.3e-13, (27.4% identity in 387 aa overlap); Rv2425c|MTCY428_22 hypothetical protein from Mycobacterium tuberculosis FASTA score: (30.7% identity in 238 aa overlap). Contains PS00213 Lipocalin signature. O53703 No conserved hypotheticals ML2479 MMAR_0662 MSMEG_0751 Mb0375c NC_000962.3 Mycobrowser_v2 CDS 446531 447046 . - 0 Rv0369c Rv0369c Function unknown; probably involved in cellular metabolism. Possible membrane oxidoreductase Rv0369c, (MTV036.04c), len: 171 aa. Possible membrane protein oxidoreductase, similar to ORF 4 of the Pseudomonas thermocarboxydovorans protein of cutA-cutB-cutC gene cluster: X77931|PTC2CUTAC_4 ORF4 from Pseudomonas thermocarboxydovorans (171 aa), FASTA scores: opt: 226, E(): 9.8e-08, (31.3% identity in 166 aa overlap). Also similar to MTV036.05, MTV036.08, MTV036.09, and MTV026.10. O53704 No intermediary metabolism and respiration ML2480 Mb0376c NC_000962.3 Mycobrowser_v2 CDS 1589386 1589901 . + 0 Rv1413 Rv1413 Function unknown Conserved hypothetical protein Rv1413, (MTCY21B4.30), len: 171 aa. Conserved hypothetical protein, similar to part of AB010956|AB010956_1 metal-activated pyridoxal enzyme from Arthrobacter sp. (379 aa), FASTA scores: opt: 187, E(): 0.00026, (29.0% identity in 162 aa overlap). P64843 No conserved hypotheticals Mb1448 NC_000962.3 Mycobrowser_v2 CDS 532396 532911 . + 0 Rv0443 Rv0443 Function unknown Conserved protein Rv0443, (MTV037.07), len: 171 aa. Conserved protein, highly similar to AL049863|SC5H1_23|T35339 hypothetical protein from Streptomyces coelicolor (171 aa), FASTA scores: opt: 561, E(): 2.3e-32, (49.7% identity in 165 aa overlap); and CAC42482.1|AJ318385 hypothetical protein from Amycolatopsis mediterranei (163 aa). O53728 No conserved hypotheticals Mb0451 NC_000962.3 Mycobrowser_v2 CDS 447147 448043 . - 0 Rv0370c Rv0370c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0370c, (MTV036.05c), len: 298 aa. Possible oxidoreductase, similar to many hypothetical proteins, but also similar to ORF4|X82447|OCCOXMSL4_4 Protein of coxMSL gene cluster from Pseudomonas/Oligotropha carboxidovorans (295 aa), FASTA scores: opt: 851, E(): 0, (48.2% identity in 282 aa overlap); AJ224684|BJAJ4684_3 cooxS from Bradyrhizobium japonicum (302 aa), FASTA scores: opt: 881, E(): 0, (47.6% identity in 290 aa overlap). Also highly similar to MTCY428_21 from Mycobacterium tuberculosis. Contains PS00017 ATP/GTP-binding site motif A (P-loop). O53705 No intermediary metabolism and respiration ML2481 MMAR_0660 MSMEG_0748 Mb0377c NC_000962.3 Mycobrowser_v2 CDS 448040 448633 . - 0 Rv0371c Rv0371c Function unknown Conserved hypothetical protein Rv0371c, (MTV036.06c), len: 197 aa. Conserved hypothetical protein, similar to other hypothetical proteins e.g. AL132824|SCAH10.09c|CAB60163.1|AL132824 hypothetical protein from Streptomyces coelicolor (207 aa), FASTA scores: opt: 247, E(): 4.5e-09, (32.3% identity in 195 aa overlap). Also weak similarity with YURE|D70017|Z99120|BSUB0017_134 hypothetical protein yurE from Bacillus subtilis (197 aa), FASTA scores: opt: 217, E(): 2.5e-08, (27.0% identity in 174 aa overlap). O53706 No conserved hypotheticals ML2482 MMAR_0659 Mb0378c NC_000962.3 Mycobrowser_v2 RBS 1240178 1240180 . + 0 Rv1115 Rv1115 Unknown Possible exported protein Rv1115, (MTCY22G8.04), len: 232 aa. Possible exported protein, contains possible N-terminal signal sequence. O06567 No cell wall and cell processes ML0940 MMAR_4345 Mb1145 NC_000962.3 Mycobrowser_v2 CDS 1240187 1240885 . + 0 Rv1115 Rv1115 Unknown Possible exported protein Rv1115, (MTCY22G8.04), len: 232 aa. Possible exported protein, contains possible N-terminal signal sequence. O06567 No cell wall and cell processes ML0940 MMAR_4345 Mb1145 NC_000962.3 Mycobrowser_v2 CDS 448630 449385 . - 0 Rv0372c Rv0372c Function unknown Conserved hypothetical protein Rv0372c, (MTV036.07c), len: 251 aa. Conserved hypothetical protein, showing some similarity with CAB76248.1|X82447|COXF CoxF protein from Pseudomonas/Oligotropha carboxidovorans (280 aa); AJ224684|BJAJ4684_6 cooxS from Bradyrhizobium japonicum (176 aa), FASTA scores: opt: 186, E(): 1.6e-05, (41.1% identity in 95 aa overlap). Also similar to upstream ORF Rv0376c from Mycobacterium tuberculosis (380 aa), FASTA scores: E(): 6.8e-07, (31.0% identity in 277 aa overlap). O53707 No conserved hypotheticals ML2483 MMAR_0658 MSMEG_0747 Mb0379c NC_000962.3 Mycobrowser_v2 RBS 449392 449395 . - 0 Rv0372c Rv0372c Function unknown Conserved hypothetical protein Rv0372c, (MTV036.07c), len: 251 aa. Conserved hypothetical protein, showing some similarity with CAB76248.1|X82447|COXF CoxF protein from Pseudomonas/Oligotropha carboxidovorans (280 aa); AJ224684|BJAJ4684_6 cooxS from Bradyrhizobium japonicum (176 aa), FASTA scores: opt: 186, E(): 1.6e-05, (41.1% identity in 95 aa overlap). Also similar to upstream ORF Rv0376c from Mycobacterium tuberculosis (380 aa), FASTA scores: E(): 6.8e-07, (31.0% identity in 277 aa overlap). O53707 No conserved hypotheticals ML2483 MMAR_0658 MSMEG_0747 Mb0379c NC_000962.3 Mycobrowser_v2 CDS 449404 451803 . - 0 Rv0373c Rv0373c Function unknown; probably involved in cellular metabolism [catalytic activity: CO + H(2)O + acceptor = CO(2) + reduced acceptor]. Probable carbon monoxyde dehydrogenase (large chain) Rv0373c, (MTV036.08c), len: 799 aa. Probable carbon monoxide dehydrogenase, large chain, highly similar to others e.g. AAD00363.1| U80806|CUTL carbon monoxide dehydrogenase large subunit CutL protein from Hydrogenophaga pseudoflava (803 aa); S49124|509391|CAA54902.1|X77931|1094915|2107180C|CUTA carbon-monoxide dehydrogenase large chain (cut operon) from Pseudomonas thermocarboxydovorans (842 aa); C56279|809566|CAA57829.1|X82447|OCCOXMSL4_3|COXL carbon-monoxide dehydrogenase large chain (cluster coxMSL) from Pseudomonas/Oligotropha carboxydovorans (809 aa), FASTA scores: opt: 2484, E(): 0, (56.0% identity in 804 aa overlap); etc. O53708 No intermediary metabolism and respiration MMAR_0657 MSMEG_0746 Mb0380c NC_000962.3 Mycobrowser_v2 RBS 451811 451814 . - 0 Rv0373c Rv0373c Function unknown; probably involved in cellular metabolism [catalytic activity: CO + H(2)O + acceptor = CO(2) + reduced acceptor]. Probable carbon monoxyde dehydrogenase (large chain) Rv0373c, (MTV036.08c), len: 799 aa. Probable carbon monoxide dehydrogenase, large chain, highly similar to others e.g. AAD00363.1| U80806|CUTL carbon monoxide dehydrogenase large subunit CutL protein from Hydrogenophaga pseudoflava (803 aa); S49124|509391|CAA54902.1|X77931|1094915|2107180C|CUTA carbon-monoxide dehydrogenase large chain (cut operon) from Pseudomonas thermocarboxydovorans (842 aa); C56279|809566|CAA57829.1|X82447|OCCOXMSL4_3|COXL carbon-monoxide dehydrogenase large chain (cluster coxMSL) from Pseudomonas/Oligotropha carboxydovorans (809 aa), FASTA scores: opt: 2484, E(): 0, (56.0% identity in 804 aa overlap); etc. O53708 No intermediary metabolism and respiration MMAR_0657 MSMEG_0746 Mb0380c NC_000962.3 Mycobrowser_v2 CDS 466672 467406 . - 0 Rv0387c Rv0387c Function unknown Conserved hypothetical protein Rv0387c, (MTV036.22c), len: 244 aa. Conserved hypothetical protein, showing some similarity to MTCI237.20c, and M17282|HUMEL20_1 Human elastin gene, exon 1, Elastin (687 aa), FASTA scores: opt: 193, E(): 0.35, (34.4% identity in 189 aa overlap). O53721 No conserved hypotheticals ML0278 Mb0394c NC_000962.3 Mycobrowser_v2 CDS 451800 452279 . - 0 Rv0374c Rv0374c Function unknown; probably involved in cellular metabolism [catalytic activity: CO + H(2)O + acceptor = CO(2) + reduced acceptor]. Probable carbon monoxyde dehydrogenase (small chain) Rv0374c, (MTV036.09c), len: 159 aa. Probable carbon monoxide dehydrogenase, small chain, highly similar to others e.g. B56279|5822285|X82447|OCCOXMSL4_2|COXS carbon-monoxide dehydrogenase small chain from Pseudomonas/Oligotropha carboxydovorans (166 aa), FASTA scores: opt: 662, E(): 0, (59.3% identity in 150 aa overlap); CAA12063.1|AJ224684 putative carbon monoxide dehydrogenase small subunit from Bradyrhizobium japonicum (161 aa); S49123|509390|CAA54901.1|X77931|CUTC carbon-monoxide dehydrogenase small chain from Pseudomonas thermocarboxydovorans (163 aa); etc. O53709 No intermediary metabolism and respiration ML2499 MMAR_0656 MSMEG_0745 Mb0381c NC_000962.3 Mycobrowser_v2 RBS 452286 452289 . - 0 Rv0374c Rv0374c Function unknown; probably involved in cellular metabolism [catalytic activity: CO + H(2)O + acceptor = CO(2) + reduced acceptor]. Probable carbon monoxyde dehydrogenase (small chain) Rv0374c, (MTV036.09c), len: 159 aa. Probable carbon monoxide dehydrogenase, small chain, highly similar to others e.g. B56279|5822285|X82447|OCCOXMSL4_2|COXS carbon-monoxide dehydrogenase small chain from Pseudomonas/Oligotropha carboxydovorans (166 aa), FASTA scores: opt: 662, E(): 0, (59.3% identity in 150 aa overlap); CAA12063.1|AJ224684 putative carbon monoxide dehydrogenase small subunit from Bradyrhizobium japonicum (161 aa); S49123|509390|CAA54901.1|X77931|CUTC carbon-monoxide dehydrogenase small chain from Pseudomonas thermocarboxydovorans (163 aa); etc. O53709 No intermediary metabolism and respiration ML2499 MMAR_0656 MSMEG_0745 Mb0381c NC_000962.3 Mycobrowser_v2 CDS 476679 477320 . - 0 Rv0398c Rv0398c Unknown Possible secreted protein Rv0398c, (MTCY04D9.10c), len: 213 aa. Possible secreted protein, sharing no homology with other proteins. Has potential signal sequence with hydrophobic stretch from aa 7-25. P95206 No cell wall and cell processes ML0274 MMAR_0695 Mb0404c NC_000962.3 Mycobrowser_v2 CDS 452294 453154 . - 0 Rv0375c Rv0375c Function unknown; probably involved in cellular metabolism [catalytic activity: CO + H(2)O + acceptor = CO(2) + reduced acceptor]. Probable carbon monoxyde dehydrogenase (medium chain) Rv0375c, (MTV036.10c), len: 286 aa. Probable carbon monoxide dehydrogenase, medium chain, similar to others e.g. AAD00361.1|U80806|CUTM carbon monoxide dehydrogenase middle subunit from Hydrogenophaga pseudoflava (287 aa); S49122|509389|CAA54900.1|X77931|CUTB carbon-monoxide dehydrogenase medium chain from Pseudomonas thermocarboxydovorans (287 aa); A56279|809564|CAA57827.1|X82447|OCCOXMSL4_1|COXM|CODH carbon-monoxide dehydrogenase medium chain from Pseudomonas/Oligotropha carboxydovorans (288 aa), FASTA scores: opt: 594, E(): 0, (37.5% identity in 277 aa overlap); etc. O53710 No intermediary metabolism and respiration MMAR_0655 MSMEG_0744 Mb0382c NC_000962.3 Mycobrowser_v2 CDS 453230 454372 . - 0 Rv0376c Rv0376c Function unknown Conserved hypothetical protein Rv0376c, (MTV036.11c), len: 380 aa. Conserved hypothetical protein, highly similar to T35481|4008539|CAA22508.1|AL034492|SC6C5.10 hypothetical protein from Streptomyces coelicolor (395 aa); and AAK64260.1|AF373840_20 ORF377 hypothetical CoxI from Arthrobacter nicotinovorans (377 aa). And similar to other conserved hypothetical proteins e.g. NP_101963.1|14021136|BAB47749.1|AP002994 hypothetical protein from Mesorhizobium loti (245 aa). Note that C-terminus shows similarity with C-termini of CAB76248.1|X82447|COXF CoxF protein from Pseudomonas/Oligotropha carboxidovorans (280 aa); CAB76250.1|X82447|COXI CoxI protein from Pseudomonas/Oligotropha carboxidovorans (330 aa); and AJ224684|BJAJ4684_6 cooxS from Bradyrhizobium japonicum (176 aa), FASTA scores: E(): 1.9e-17, (47.1% identity in 138 aa overlap). Also some partial similarity with AJ224684|BJAJ4684_5 cooxS from Bradyrhizobium japonicum (107 aa), FASTA scores: opt: 321, E(): 4.2e-14, (53.3% identity in 92 aa overlap); E1184330|Z99120|YURF YURF protein from Bacillus subtilis (330 aa), FASTA scores: opt: 170, E(): 2.9e- 16, (27.5% identity in 345 aa overlap). Also similar to downstream ORF Rv0372c from Mycobacterium tuberculosis (251 aa), FASTA scores: E(): 2.1e-06, (30.7% identity in 277 aa overlap). O53711 No conserved hypotheticals MMAR_0654 MSMEG_0743 Mb0383c NC_000962.3 Mycobrowser_v2 CDS 566776 567222 . + 0 Rv0477 Rv0477 Unknown Possible conserved secreted protein Rv0477, (MTCY20G9.03), len: 148 aa. Possible conserved secreted protein, equivalent to CAC31969.1|AL583925 hypothetical protein from Mycobacterium leprae (123 aa). Also similar to G83406|PA1914 conserved hypothetical protein from Pseudomonas aeruginosa (408 aa). Contains possible N-terminal signal sequence. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P64697 No cell wall and cell processes ML2452 MMAR_0802 MSMEG_0921 Mb0487 NC_000962.3 Mycobrowser_v2 CDS 467459 468001 . - 0 Rv0388c PPE9 Function unknown PPE family protein PPE9 Rv0388c, (MTV036.23c), len: 180 aa. PPE9, Member of the Mycobacterium tuberculosis PPE family, highly similar to others e.g. MTCY10G2_10|Z92539 from Mycobacterium tuberculosis (391 aa), FASTA scores: opt: 667, E(): 0, (58.3% identity in 180 aa overlap) but much shorter. Q6MX42 No PE/PPE ML0277 NC_000962.3 Mycobrowser_v2 CDS 454421 455386 . + 0 Rv0377 Rv0377 Possibly involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably LysR-family) Rv0377, (MTV036.12), len: 321 aa. Probable transcription regulator, lysR family, showing similarity with many hypothetical transcriptional regulators lysR homolog e.g. P32484|YEIE_ECOLI|M89774 hypothetical transcriptional regulator from Escherichia coli (293 aa), FASTA scores: opt: 265, E(): 4.9e-11, (28.6% identity in 266 aa overlap). Also similar to Rv2282c from Mycobacterium tuberculosis. Contains PS00044 bacterial regulatory protein lysR family signature. Seems to belong to the LysR family of transcriptional regulators. O53712 No regulatory proteins ML2484 MMAR_0653 MSMEG_0742 Mb0384 NC_000962.3 Mycobrowser_v2 CDS 455637 455858 . + 0 Rv0378 Rv0378 Function unknown Conserved hypothetical glycine rich protein Rv0378, (MTV036.13), len: 73 aa. Conserved hypothetical gly-rich protein, showing some similarity to Mycobacterium tuberculosis PE_PGRS family; also similar to MTCY06H11_16|Z85982 hypothetical glycine-rich 88.5 KD protein (1011 aa), FASTA scores: opt: 237, E(): 0.0032, (58.7% identity in 63 aa overlap); MTV043_25. O53713 No conserved hypotheticals MMAR_2795 Mb0385 NC_000962.3 Mycobrowser_v2 CDS 455977 456192 . + 0 Rv0379 secE2 Thought to be involved in protein transport (export). Possible protein transport protein SecE2 Rv0379, (MTV036.14), len: 71 aa. Possible secE2, protein transport protein, showing similarity with P27340|S61G_SULSO|SECE preprotein translocase SECE subunit (protein transport protein SEC61 gamma subunit homolog) from Sulfolobus acidocaldarius (65 aa), FASTA scores: opt: 79, E(): 4.7. (30.3% identity in 66 aa overlap); and hypothetical proteins e.g. Q9HPW4|VNG1446H hypothetical protein from Halobacterium sp. strain NRC-1 (77 aa); Q9I794|PA0038 hypothetical protein from Pseudomonas aeruginosa (71 aa); etc. Also highly similar to U85467|MTU85467_1 hypothetical Mycobacterium tuberculosis protein from a patient isolate (116 aa), FASTA scores: opt: 443, E(): 7.7e-29, (98.6% identity in 71 aa overlap). Note that for Rv0379|MTV036.14, a translation initiation region different to the one in U85467|MTU85467_1 was chosen. Could be a part of the prokaryotic protein translocation apparatus which comprise SECA|Rv3240c, SECD|Rv2587c, SECE|Rv0638, SECF|Rv2586c, SECG|Rv1440 and SECY|Rv0732. Q6MX43 No cell wall and cell processes MMAR_0652 Mb0386 NC_000962.3 Mycobrowser_v2 CDS 456268 456819 . - 0 Rv0380c Rv0380c Possibly causes methylation of RNA. Possible RNA methyltransferase (RNA methylase) Rv0380c, (MTV036.15c), len: 183 aa. Possible RNA methyltransferase, equivalent to CAC32002.1|AL583925 possible RNA methyltransferase from Mycobacterium leprae (182 aa). Also some similarity with others methyltransferases e.g. P19396|TRMH_ECOLI|78514|JV0043 tRNA (guanosine-2'-O-)-methyltransferase (tRNA methyltransferase) from Escherichia coli (229 aa), FASTA scores: opt: 227, E(): 1.4e-09, (28.9% identity in 166 aa overlap). Also similar to Rv0881, Rv3579c, Rv1644 from Mycobacterium tuberculosis. O53715 No intermediary metabolism and respiration ML2485 MMAR_0651 MSMEG_0739 Mb0387c NC_000962.3 Mycobrowser_v2 CDS 456915 457823 . - 0 Rv0381c Rv0381c Unknown Hypothetical protein Rv0381c, (MTV036.16c), len: 302 aa. Hypothetical unknown protein. Equivalent to AAK44616.1 from Mycobacterium tuberculosis strain CDC1551 (254 aa) but longer 48 aa. O53716 No conserved hypotheticals ML2486 MMAR_0650 MSMEG_0738 Mb0388c NC_000962.3 Mycobrowser_v2 RBS 2307807 2307812 . + 0 Rv2050 Rv2050 Unknown Conserved protein Rv2050, (MTV018.37), len: 111 aa. Conserved protein, similar to many. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53492 No conserved hypotheticals ML1439 MMAR_3027 MSMEG_3858 Mb2076 NC_000962.3 Mycobrowser_v2 CDS 2307821 2308156 . + 0 Rv2050 Rv2050 Unknown Conserved protein Rv2050, (MTV018.37), len: 111 aa. Conserved protein, similar to many. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53492 No conserved hypotheticals ML1439 MMAR_3027 MSMEG_3858 Mb2076 NC_000962.3 Mycobrowser_v2 CDS 474122 474841 . - 0 Rv0394c Rv0394c Unknown Possible secreted protein Rv0394c, (MTCY04D9.06c), len: 239 aa. Possible secreted protein, sharing no homology with other proteins. Has hydrophobic stretch at its N-terminus. P95202 No cell wall and cell processes Mb0400c NC_000962.3 Mycobrowser_v2 CDS 457841 458380 . - 0 Rv0382c pyrE Involved in pyrimidine biosynthesis (at the fifth step) [catalytic activity: orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate]. Probable orotate phosphoribosyltransferase PyrE (OPRT) (oprtase) Rv0382c, (MTV036.17c), len: 179 aa. Probable pyrE, orotate phosphoribosyltransferase, equivalent to CAC32004.1|AL583925 probable purine/pyrimidine phosphoribosyltransferase from Mycobacterium leprae (179 aa). Also highly similar to many others e.g. T36540|4753874|CAB42037.1|AL049754|SCH10.28c probable orotate phosphoribosyltransferase from Streptomyces coelicolor (182 aa); H69115|2622996|AAB86326.1|AE000938_10|MTH1860 probable orotate phosphoribosyltransferase from Methanobacterium thermoautotrophicum (180 aa), FASTA scores: opt: 389, E(): 2.7e-20, (40.7% identity in 172 aa overlap); O08359|PYRE_SULAC|2065444|CAA73352.1|Y12822 orotate phosphoribosyltransferase from Sulfolobus acidocaldarius (197 aa); etc. Note that also similar to other puridine 5'-monophosphate synthases (umpA genes; UMP synthases), generally in N-terminus that corresponds to orotate phosphoribosyltransferase activity. Contains PS00589 PTS HPR component serine phosphorylation site signature. Belongs to the purine/pyrimidine phosphoribosyltransferase family. Note that previously known as umpA. Nucleotide position 458282 in the genome sequence has been corrected, A:G resulting in Y33Y. P0A5U0 No intermediary metabolism and respiration ML2487 MMAR_0649 Mb0389c NC_000962.3 Mycobrowser_v2 RBS 458387 458391 . - 0 Rv0382c pyrE Involved in pyrimidine biosynthesis (at the fifth step) [catalytic activity: orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate]. Probable orotate phosphoribosyltransferase PyrE (OPRT) (oprtase) Rv0382c, (MTV036.17c), len: 179 aa. Probable pyrE, orotate phosphoribosyltransferase, equivalent to CAC32004.1|AL583925 probable purine/pyrimidine phosphoribosyltransferase from Mycobacterium leprae (179 aa). Also highly similar to many others e.g. T36540|4753874|CAB42037.1|AL049754|SCH10.28c probable orotate phosphoribosyltransferase from Streptomyces coelicolor (182 aa); H69115|2622996|AAB86326.1|AE000938_10|MTH1860 probable orotate phosphoribosyltransferase from Methanobacterium thermoautotrophicum (180 aa), FASTA scores: opt: 389, E(): 2.7e-20, (40.7% identity in 172 aa overlap); O08359|PYRE_SULAC|2065444|CAA73352.1|Y12822 orotate phosphoribosyltransferase from Sulfolobus acidocaldarius (197 aa); etc. Note that also similar to other puridine 5'-monophosphate synthases (umpA genes; UMP synthases), generally in N-terminus that corresponds to orotate phosphoribosyltransferase activity. Contains PS00589 PTS HPR component serine phosphorylation site signature. Belongs to the purine/pyrimidine phosphoribosyltransferase family. Note that previously known as umpA. Nucleotide position 458282 in the genome sequence has been corrected, A:G resulting in Y33Y. P0A5U0 No intermediary metabolism and respiration ML2487 MMAR_0649 Mb0389c NC_000962.3 Mycobrowser_v2 CDS 458461 459315 . - 0 Rv0383c Rv0383c Unknown Possible conserved secreted protein Rv0383c, (MTV036.18c), len: 284 aa. Possible conserved secreted protein, with hydrophobic stretch in N-terminus and Pro-rich C-terminus. Equivalent to CAC32006.1|AL583925 possible secreted protein from Mycobacterium leprae (286 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53718 No cell wall and cell processes ML2489 MMAR_0647 MSMEG_0736 Mb0390c NC_000962.3 Mycobrowser_v2 CDS 1241003 1241188 . + 0 Rv1116 Rv1116 Unknown Hypothetical protein Rv1116, (MTCY22G8.05), len: 61 aa. Hypothetical unknown protein. O06568 No conserved hypotheticals Mb1146 NC_000962.3 Mycobrowser_v2 CDS 459456 462002 . - 0 Rv0384c clpB Thought to be an ATPase subunit of an intracellular ATP-dependent protease. Seems to be regulated positively by sigh (Rv3223c product) and negatively by HSPR (Rv0353 product). Probable endopeptidase ATP binding protein (chain B) ClpB (ClpB protein) (heat shock protein F84.1) Rv0384c, (MTV036.19c), len: 848 aa. Probable clpB (alternate gene name: htpM), endopeptidase ATP-binding protein, chain B, equivalent to AC32007.1|AL583925 heat shock protein from Mycobacterium leprae (848 aa). Also highly similar to others e.g. P53532|CLPB_CORGL|1163118|AAB49540.1|U43536|CGU43536_1 CLPB protein (heat-inducible expression) from Corynebacterium glutamicum (852 aa), FASTA scores: opt: 4113, E(): 0, (74.5% identity in 846 aa overlap); T36551|4753885|CAB42048.1|AL049754|clpB|SCOEDB|SCH10.39c probable ATP-dependent proteinase ATP-binding chain from Streptomyces coelicolor (853 aa); P03815|CLPB_ECOLI|1788943|AAC75641.1|AE000345 CLPB protein (heat shock protein F84.1) from Escherichia coli strains K12 and O157:H7 (857 aa); etc. Also similar to Rv3596c|ClpC from Mycobacterium tuberculosis. Contains PS00870 and PS00871 Chaperonins clpA/B signatures and two PS000017 ATP/GTP-binding site motives a (P-loop). Belongs to the CLPA/CLPB family. Contains probable coiled-coil domain from aa 411-503. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P63288 No virulence, detoxification, adaptation ML2490 MMAR_0645 MSMEG_0732 Mb0391c NC_000962.3 Mycobrowser_v2 RBS 462008 462011 . - 0 Rv0384c clpB Thought to be an ATPase subunit of an intracellular ATP-dependent protease. Seems to be regulated positively by sigh (Rv3223c product) and negatively by HSPR (Rv0353 product). Probable endopeptidase ATP binding protein (chain B) ClpB (ClpB protein) (heat shock protein F84.1) Rv0384c, (MTV036.19c), len: 848 aa. Probable clpB (alternate gene name: htpM), endopeptidase ATP-binding protein, chain B, equivalent to AC32007.1|AL583925 heat shock protein from Mycobacterium leprae (848 aa). Also highly similar to others e.g. P53532|CLPB_CORGL|1163118|AAB49540.1|U43536|CGU43536_1 CLPB protein (heat-inducible expression) from Corynebacterium glutamicum (852 aa), FASTA scores: opt: 4113, E(): 0, (74.5% identity in 846 aa overlap); T36551|4753885|CAB42048.1|AL049754|clpB|SCOEDB|SCH10.39c probable ATP-dependent proteinase ATP-binding chain from Streptomyces coelicolor (853 aa); P03815|CLPB_ECOLI|1788943|AAC75641.1|AE000345 CLPB protein (heat shock protein F84.1) from Escherichia coli strains K12 and O157:H7 (857 aa); etc. Also similar to Rv3596c|ClpC from Mycobacterium tuberculosis. Contains PS00870 and PS00871 Chaperonins clpA/B signatures and two PS000017 ATP/GTP-binding site motives a (P-loop). Belongs to the CLPA/CLPB family. Contains probable coiled-coil domain from aa 411-503. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P63288 No virulence, detoxification, adaptation ML2490 MMAR_0645 MSMEG_0732 Mb0391c NC_000962.3 Mycobrowser_v2 CDS 462135 463307 . + 0 Rv0385 Rv0385 Function unknown; probably involved in cellular metabolism. Probable monooxygenase Rv0385, (MTV036.20), len: 390 aa. Probable monooxygenase, similar to T37003|5738846|CAB52917.1|AL109949 probable flavohemoprotein from Streptomyces coelicolor (435 aa); and similar in part (C-termini) to various monooxygenases e.g. P19734|DMPP_PSESP|94993|F37831 phenol hydroxylase P5 protein (phenol 2-monooxygenase P5 component) from Pseudomonas putida (353 aa), FASTA scores: opt: 363, E(): 4.2e-16, (31.8% identity in 255 aa overlap); S47292|2120861|pir|S70085 phenol 2-monooxygenase chain mopP from Acinetobacter calcoaceticus (350 aa); P21394|XYLA_PSEPU|94933|B37316 xylene monooxygenase electron transfer component [includes: ferredoxin; ferredoxin--NAD(+) reductase] from Pseudomonas putida plasmid pWW0 (350 aa); AAC38360.1|AF043544|NtnMA|ntnA reductase component of 4-nitrotoluene monooxygenase from Pseudomonas sp. (328 aa); etc. Q7ARS9 No intermediary metabolism and respiration ML2492 MMAR_0644 MSMEG_0719 Mb0392 NC_000962.3 Mycobrowser_v2 RBS 463402 463405 . + 0 Rv0386 Rv0386 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably LuxR/UhpA-family) Rv0386, (MTV036.21), len: 1085 aa. Probable regulatory protein, LuxR/uhpA family, highly similar to CAC30706.1|AL583923 possible transcriptional regulator from Mycobacterium leprae (1106 aa). Also similar in part to other regulatory proteins e.g. CAB95788.1|AL359949 putative multi-domain regulatory protein from Streptomyces coelicolor (780 aa); N-terminus of CAB92369.1|AL356612 putative AfsR-like regulatory protein from Streptomyces coelicolor (1114 aa); N-terminus of NP_107139.1|14026327|BAB52925.1|AP003009 transcriptional regulator from Mesorhizobium loti (952 aa); AFSR_STRCO|P25941 regulatory protein afsr from Streptomyces coelicolor (993 aa), FASTA scores: opt: 224, E() : 1.1e-06, (26.1% identity in 867 aa overlap); etc. Also similar to many putative Mycobacterium tuberculosis regulatory proteins e.g. AL0212|MTV008_44 (1137 aa), FASTA scores: opt: 3756, E(): 0, (56.7% identity in 1089 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00622 Bacterial regulatory proteins, luxR family signature and probable helix-turn-helix motif at aa 1042-1063 (Score 1025, +2.68 S D). Belongs to the LuxR/UhpA family of transcriptional regulators. O53720 No regulatory proteins Mb0393 NC_000962.3 Mycobrowser_v2 CDS 463411 466668 . + 0 Rv0386 Rv0386 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably LuxR/UhpA-family) Rv0386, (MTV036.21), len: 1085 aa. Probable regulatory protein, LuxR/uhpA family, highly similar to CAC30706.1|AL583923 possible transcriptional regulator from Mycobacterium leprae (1106 aa). Also similar in part to other regulatory proteins e.g. CAB95788.1|AL359949 putative multi-domain regulatory protein from Streptomyces coelicolor (780 aa); N-terminus of CAB92369.1|AL356612 putative AfsR-like regulatory protein from Streptomyces coelicolor (1114 aa); N-terminus of NP_107139.1|14026327|BAB52925.1|AP003009 transcriptional regulator from Mesorhizobium loti (952 aa); AFSR_STRCO|P25941 regulatory protein afsr from Streptomyces coelicolor (993 aa), FASTA scores: opt: 224, E() : 1.1e-06, (26.1% identity in 867 aa overlap); etc. Also similar to many putative Mycobacterium tuberculosis regulatory proteins e.g. AL0212|MTV008_44 (1137 aa), FASTA scores: opt: 3756, E(): 0, (56.7% identity in 1089 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00622 Bacterial regulatory proteins, luxR family signature and probable helix-turn-helix motif at aa 1042-1063 (Score 1025, +2.68 S D). Belongs to the LuxR/UhpA family of transcriptional regulators. O53720 No regulatory proteins Mb0393 NC_000962.3 Mycobrowser_v2 RBS 468323 468326 . + 0 Rv0389 purT Involved in third step (first of two transformylation reactions) in de novo purine biosynthesis. This is an alternative enzyme to the PURN|Rv0956 gar transformylase (5'-phosphoribosylglycinamide formyltransferase). Catalyzes two reactions: the first one is the production of beta-formyl glycinamide ribonucleotide (gar) from formate, ATP and beta gar; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [catalytic activity: formate + ATP + 5'-phospho-ribosylglycinamide = 5'-phosphoribosyl-N-formylglycinamide + ADP + pyrophosphate]. Probable phosphoribosylglycinamide formyltransferase 2 PurT (GART 2) (gar transformylase 2) (5'-phosphoribosylglycinamide transformylase 2) (formate-dependent gar transformylase) Rv0389, (MTCY04D9.01, MTV036.24), len: 419 aa. Probable purT, phosphoribosylglycinamide formyltransferase 2, similar to others e.g. P33221|PURT_ECOLI|B1849 phosphoribosylglycinamide formyltransferase 2 from Escherichia coli strain K-12 (391 aa), FASTA scores: opt: 481, E(): 1.3e-22, (40.1% identity in 379 aa overlap); etc. Belongs to the PurK / PurT family. Cofactor: magnesium. P95197 No intermediary metabolism and respiration MMAR_0686 MSMEG_0766 Mb0395 NC_000962.3 Mycobrowser_v2 CDS 468335 469594 . + 0 Rv0389 purT Involved in third step (first of two transformylation reactions) in de novo purine biosynthesis. This is an alternative enzyme to the PURN|Rv0956 gar transformylase (5'-phosphoribosylglycinamide formyltransferase). Catalyzes two reactions: the first one is the production of beta-formyl glycinamide ribonucleotide (gar) from formate, ATP and beta gar; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [catalytic activity: formate + ATP + 5'-phospho-ribosylglycinamide = 5'-phosphoribosyl-N-formylglycinamide + ADP + pyrophosphate]. Probable phosphoribosylglycinamide formyltransferase 2 PurT (GART 2) (gar transformylase 2) (5'-phosphoribosylglycinamide transformylase 2) (formate-dependent gar transformylase) Rv0389, (MTCY04D9.01, MTV036.24), len: 419 aa. Probable purT, phosphoribosylglycinamide formyltransferase 2, similar to others e.g. P33221|PURT_ECOLI|B1849 phosphoribosylglycinamide formyltransferase 2 from Escherichia coli strain K-12 (391 aa), FASTA scores: opt: 481, E(): 1.3e-22, (40.1% identity in 379 aa overlap); etc. Belongs to the PurK / PurT family. Cofactor: magnesium. P95197 No intermediary metabolism and respiration MMAR_0686 MSMEG_0766 Mb0395 NC_000962.3 Mycobrowser_v2 CDS 469591 470013 . + 0 Rv0390 Rv0390 Function unknown Conserved protein Rv0390, (MTCY04D9.02), len: 140 aa. Conserved protein, equivalent to AL023514|MLCB4_11|CAA18942.1|AL023514 hypothetical protein from Mycobacterium leprae (147 aa), FASTA scores: opt: 778, E(): 0, (79.0% identity in 138 aa overlap). Also similar to hypothetical proteins from several Rickettsia species. P95198 No conserved hypotheticals ML0276 MMAR_0687 MSMEG_0768 Mb0396 NC_000962.3 Mycobrowser_v2 CDS 470010 471230 . + 0 Rv0391 metZ Involved in methionine biosynthesis. Converts O-succinylhomoserine into homocysteine. Probable O-succinylhomoserine sulfhydrylase MetZ (OSH sulfhydrylase) Rv0391, (MTCY04D9.03), len: 406 aa. Probable metZ, O-succinylhomoserine sulfhydrylase, equivalent, but shorter 20 aa in N-terminus, to AA18941.1|AL023514 O-succinylhomoserine sulfhydrylase from Mycobacterium leprae (426 aa). Also highly similar to others e.g. METZ_PSEAE|P55218 o-succinylhomoserine sulfhydrylase from Pseudomonas aeruginosa (403 aa), FASTA scores: opt: 1175, E(): 0, (47.2% identity in 392 aa overlap); etc. Belongs to the trans-sulfuration enzymes family. Could also be a cystathionine gamma-synthase. P95199 No intermediary metabolism and respiration ML0275 MMAR_0688 MSMEG_0769 Mb0397 NC_000962.3 Mycobrowser_v2 CDS 471227 472639 . - 0 Rv0392c ndhA Transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Does not couple the redox reaction to proton translocation [catalytic activity: NADH + acceptor = NAD+ + reduced acceptor]. Probable membrane NADH dehydrogenase NdhA Rv0392c, (MTCY04D9.04c), len: 470 aa. Probable ndhA, membrane NADH dehydrogenase, equivalent to many e.g. AF038423|AF038423_1 NADH dehydrogenase from Mycobacterium smegmatis (457 aa), FASTA scores: opt: 1991, E(): 0, (67.9% identity in 458 aa overlap); MLCB1788_3 NADH dehydrogenase from Mycobacterium leprae (466 aa), FASTA score: (62.5% identity in 467 aa overlap). Also similar to others from several organisms e.g. P00393|DHNA_ECOLI|66211|581140|CAA23586.1|V00306 NADH dehydrogenase from Escherichia coli (434 aa); and Rv0392c|ndhB from Mycobacterium tuberculosis. Has hydrophobic stretch in C-terminus. Belongs to the NADH dehydrogenase family. P95200 No intermediary metabolism and respiration MMAR_0689 Mb0398c NC_000962.3 Mycobrowser_v2 CDS 472781 474106 . + 0 Rv0393 Rv0393 Function unknown Conserved 13E12 repeat family protein Rv0393, (MTCY04D9.05), len: 441 aa. Member of Mycobacterium tuberculosis 13E12 repeat family of conserved proteins, similar to many e.g. Rv1148c, Rv1945, Rv3467, Rv0336|MTCY279_3 (503 aa), FASTA scores: E(): 0, (61.1% identity in 347 aa overlap). P95201 No insertion seqs and phages MMAR_1213,MMAR_2226 Mb0399 NC_000962.3 Mycobrowser_v2 CDS 477327 478556 . - 0 Rv0399c lpqK Unknown Possible conserved lipoprotein LpqK Rv0399c, (MTCY04D9.11c), len: 409 aa. Possible lpqK, conserved lipoprotein, showing some similarity to penicillin binding proteins and various peptidases e.g. DAC_STRSQ|P15555 d-alanyl-d-alanine carboxypeptidase protein (406 aa), FASTA scores: opt: 348, E(): 5.6e-16, (29.2% identity in 301 aa overlap). Also similar to other Mycobacterium tuberculosis PBPs and esterases. Has possible N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein lipid attachment site (PS00013). P95207 No cell wall and cell processes MMAR_0696 Mb0405c NC_000962.3 Mycobrowser_v2 CDS 478566 479753 . - 0 Rv0400c fadE7 Function unknown, but involved in lipid degradation. Acyl-CoA dehydrogenase FadE7 Rv0400c, (MTCY04D9.12c), len: 395 aa. Probable fadE7, acyl-CoA dehydrogenase, similar to many e.g. CAC12923.1|AL445403 putative acyl CoA dehydrogenase from Streptomyces coelicolor (397 aa); G624219 glutaryl-CoA dehydrogenase precursor (438 aa), FASTA scores: opt: 1161, E(): 0, (48.1% identity in 391 aa overlap); etc. P95208 No lipid metabolism ML0273 Mb0406c NC_000962.3 Mycobrowser_v2 CDS 480355 483231 . - 0 Rv0402c mmpL1 Unknown. Thought to be involved in fatty acid transport. Probable conserved transmembrane transport protein MmpL1 Rv0402c, (MTCY04D9.15c), len: 958 aa. Probable mmpL1, conserved transmembrane transport protein (see Tekaia et al., 1999), member of RND superfamily, highly similar to other Mycobacterial proteins e.g. YV34_MYCTU|Q11171 hypothetical 106.2 kDa membrane protein from Mycobacterium tuberculosis (968 aa), FASTA scores: opt: 3551, E(): 0, (55.4% identity in 933aa overlap); YV34_MYCLE|P54881 hypothetical 105.2 kDa protein from Mycobacterium leprae (959 aa), FASTA scores: opt: 3615, E(): 0, (55.5% identity in 941 aa overlap); etc. Highly similar to many other mycobacterial MmpL proteins from Mycobacterium tuberculosis and Mycobacterium leprae e.g. Rv0450c, Rv0676c, Rv0507, etc. Belongs to the MmpL family. P95211 No cell wall and cell processes MMAR_0704,MMAR_1722 Mb0408c,Mb0409c NC_000962.3 Mycobrowser_v2 CDS 483228 483656 . - 0 Rv0403c mmpS1 Unknown Probable conserved membrane protein MmpS1 Rv0403c, (MTCY04D9.16c), len: 142 aa. Probable mmpS1, conserved membrane protein (see citation below), highly similar to other Mycobacterial proteins e.g. YV33_MYCLE|P54880 hypothetical 16.9 kDa protein from Mycobacterium leprae (154 aa), FASTA scores: opt: 458, E(): 1.6e-26, (46.9% identity in 143 aa overlap); YV33_MYCTU|Q11170 hypothetical 15.9 kDa protein from Mycobacterium tuberculosis (147 aa), FASTA scores: opt: 362, E(): 1.1e-19, (42.1% identity in 140 aa overlap); etc. Also similar to other MmpS proteins from Mycobacterium tuberculosis e.g. Rv0677c, Rv0451c, etc. Belongs to the MmpS family. Predicted to be an outer membrane protein (See Song et al., 2008). P0A5K0 No cell wall and cell processes MMAR_0705,MMAR_1723 Mb0410c NC_000962.3 Mycobrowser_v2 CDS 483977 485734 . + 0 Rv0404 fadD30 Function unknown, but involved in lipid degradation. Fatty-acid-AMP ligase FadD30 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) Rv0404, (MTCY04D9.17-MTCY22G10.00), len: 585 aa. fadD30, fatty-acid-AMP synthetase, similar to many e.g. MBU75685_1|AAB52538.1|U75685 acyl-CoA synthase from Mycobacterium bovis (582 aa); MASC_MYCLE|P54200 masc protein from Mycobacterium leprae (372 aa), FASTA scores: opt: 888, E(): 0, (44.2% identity in 342 aa overlap). Also similar to Y06J_MYCTU|Q10976 hypothetical 67.9 kDa protein (626 aa), FASTA scores: opt: 1463, E(): 0, (42.4% identity in 568 aa overlap). P95213 No lipid metabolism MMAR_0706 Mb0411 NC_000962.3 Mycobrowser_v2 CDS 485731 489939 . + 0 Rv0405 pks6 Polyketide synthase possibly involved in lipid synthesis. Probable membrane bound polyketide synthase Pks6 Rv0405, (MTCY22G10.01), len: 1402 aa. Probable pks6, membrane-bound polyketide synthase (see citation below), highly similar to others e.g. CAC29643.1|AL583917 putative polyketide synthase from Mycobacterium leprae (2103 aa); Y06K_MYCTU|Q10977 probable polyketide synthase (1876 aa), FASTA scores: opt: 2303, E(): 0, (38.7% identity in 1232 aa overlap); etc. Contains PS00606 Beta-ketoacyl synthases active site, 2 x PS00017 ATP/GTP-binding site motif A (P-loop), and PS00012 Phosphopantetheine attachment site. O86335 No lipid metabolism Mb0412,Mb0413 NC_000962.3 Mycobrowser_v2 CDS 489887 490705 . - 0 Rv0406c Rv0406c Function unknown Beta lactamase like protein Rv0406c, (MTCY22G10.02c), len: 272 aa. Beta-lactamase-like protein, equivalent to AAD38170.1|AF152397_1 beta-lactamase-like protein from Mycobacterium phlei (243 aa); AL023514|MLCB4_8 hypothetical protein from Mycobacterium leprae (251 aa), FASTA scores: opt: 1284, E(): 0, (74.9% identity in 243 aa overlap); and AAD38164.1|AF152394_2 beta-lactamase-like protein from Mycobacterium avium (247 aa), FASTA scores: opt: 1301, E(): 0, (74.2% identity in 244 aa overlap); etc. Also slight similarity to others beta-lactamases and hypothetical proteins e.g. P52700|BLA1_XANMA|628530|S45349 metallo-beta-lactamase L1 precursor (beta-lactamase, type II) (penicillinase) from Xanthomonas maltophilia (290 aa), FASTA scores: (34.4% identity in 96 aa overlap). Recombinant protein has beta lactamase activity (See Nampoothiri et al., 2008). O86336 No conserved hypotheticals ML0270 MMAR_0708 MSMEG_0775 Mb0414c NC_000962.3 Mycobrowser_v2 CDS 490783 491793 . + 0 Rv0407 fgd1 Catalyzes oxidation of glucose-6-phosphate to 6-phosphogluconolactone using coenzyme F420 (an *-hydroxy-5-deazaflavin derivative) as the electron acceptor. F420-dependent glucose-6-phosphate dehydrogenase Fgd1 Rv0407, (MTCY22G10.03), len: 336 aa. fgd1, F420-dependent glucose-6-phosphate dehydrogenase, equivalent to others from Mycobacteria e.g. AAD38165.1|AF152394_3 from Mycobacterium avium (336 aa), FASTA scores: opt: 2082, E(): 0, (89.9% identity in 336 aa overlap); AL023514|MLCB 4_7 from Mycobacterium leprae (336 aa), FASTA scores: opt: 2069, E(): 0, (89.0% identity in 336 aa overlap). Also similar to other dehydrogenases e.g. CAA77276.1|Y18730 F420-dependent alcohol dehydrogenase from Methanofollis liminatans (330 aa). Also similar to many proteins from Mycobacterium tuberculosis e.g. Rv0953c, Rv0791c, etc. Note that previously known as fgd. P96253 No intermediary metabolism and respiration ML0269 MMAR_0709 MSMEG_0777 Mb0415 NC_000962.3 Mycobrowser_v2 CDS 491786 493858 . + 0 Rv0408 pta Involved at the last step (of two) in the conversion of acetate to acetyl-CoA [catalytic activity: acetyl-CoA + phosphate = CoA + acetyl phosphate]. Probable phosphate acetyltransferase Pta (phosphotransacetylase) Rv0408, (MTCY22G10.04), len: 690 aa. Probable pta, phosphate acetyltransferase, highly similar to others e.g. PTA_ECOLI|P39184|11279789|JX0357|B2297 phosphate acetyltransferase from Escherichia coli strain K12 (713 aa), FASTA scores: opt: 1303, E(): 0, (38.0% identity in 718 aa overlap); etc. Belongs to the phosphate acetyltransferase and butyryltransferase family. P96254 No intermediary metabolism and respiration ML0268 MMAR_0710 MSMEG_0783 Mb0416 NC_000962.3 Mycobrowser_v2 RBS 1241625 1241627 . + 0 Rv1117 Rv1117 Function unknown Conserved protein Rv1117, (MTCY22G8.06), len: 107 aa. Conserved protein, some similarity to P94425|D50453 hypothetical protein from Bacillus subtilis (95 aa), fasta scores: opt: 128, E(): 5.1e-06, (28.3% identity in 92 aa overlap); and AL117322|SCF1.02 Streptomyces coelicolor (109 aa), FASTA scores: opt: 437, E(): 1.6e-25, (57.5% identity in 106 aa overlap). O06569 No conserved hypotheticals ML0941 MMAR_4343 MSMEG_1532 Mb1148 NC_000962.3 Mycobrowser_v2 CDS 1241633 1241956 . + 0 Rv1117 Rv1117 Function unknown Conserved protein Rv1117, (MTCY22G8.06), len: 107 aa. Conserved protein, some similarity to P94425|D50453 hypothetical protein from Bacillus subtilis (95 aa), fasta scores: opt: 128, E(): 5.1e-06, (28.3% identity in 92 aa overlap); and AL117322|SCF1.02 Streptomyces coelicolor (109 aa), FASTA scores: opt: 437, E(): 1.6e-25, (57.5% identity in 106 aa overlap). O06569 No conserved hypotheticals ML0941 MMAR_4343 MSMEG_1532 Mb1148 NC_000962.3 Mycobrowser_v2 CDS 493851 495008 . + 0 Rv0409 ackA Involved at the first step (of two) in the conversion of acetate to acetyl-CoA [catalytic activity: ATP + acetate = ADP + acetyl phosphate]. Probable acetate kinase AckA (acetokinase) Rv0409, (MTCY22G10.05), len: 385 aa. Probable ackA, acetate kinase, highly similar to others e.g. ACKA_BACSU|P37877 acetate kinase from Bacillus subtilis (395 aa), FASTA scores: opt: 974, E(): 0, (43.5% identity in 393 aa overlap); etc. Contains PS01075 Acetate and butyrate kinases family signature 1, PS00758 ArgE / dapE / ACY1/ CPG2 / yscS family signature 1. Belongs to the acetokinase family. P63409 No intermediary metabolism and respiration ML0267 MMAR_0711 MSMEG_0784 Mb0417 NC_000962.3 Mycobrowser_v2 CDS 495062 497314 . - 0 Rv0410c pknG Involved in signal transduction (via phosphorylation). Thought to regulate amino-acid uptake and stationary-phase metabolism. Phosphorylates the peptide substrate myelin basic protein (MBP) at serine residues [catalytic activity: ATP + a protein = ADP + a phosphoprotein]. Serine/threonine-protein kinase PknG (protein kinase G) (STPK G) Rv0410c, (MTCY22G10.06c), len: 750 aa. PknG, serine/threonine-protein kinase (see citations below), equivalent to PKNG_MYCLE|P57993|13092623|CAC29812.1|AL583918 probable serine/threonine-protein kinase from Mycobacterium leprae (767 aa). Also similar to others e.g. AB76890.1|AL159139 putative serine/threonine protein kinase from Streptomyces coelicolor (774 aa); etc. Contains PS00108 Serine/Threonine protein kinases active-site signature. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases. Structure of PknG with inhibitor AX20017 reveals that the inhibitor-binding pocket is shaped by a unique set of amino acid side chains not found in any human kinase (See Scherr et al., 2007). P65728 No regulatory proteins ML0304 MMAR_0713 MSMEG_0786 Mb0418c NC_000962.3 Mycobrowser_v2 CDS 520324 521454 . + 0 Rv0433 Rv0433 Function unknown Conserved hypothetical protein Rv0433, (MTCY22G10.30), len: 376 aa. Conserved hypothetical protein, similar to other hypothetical proteins e.g. P77213|YBDK_ECOLI hypothetical 41.7 KD protein from Escherichia coli strain K12 (372 aa), FASTA scores: opt: 555, E(): 2e-30, (28.2% identity in 365 aa overlap). P64693 No conserved hypotheticals ML1924 MMAR_0748 MSMEG_0836 Mb0441 NC_000962.3 Mycobrowser_v2 CDS 497314 498300 . - 0 Rv0411c glnH Involved in active transport of glutamine across the membrane (import). Interacts with the glutamine-transport system. Probable glutamine-binding lipoprotein GlnH (GLNBP) Rv0411c, (MTCY22G10.07c), len: 328 aa. Probable glnH, glutamine-binding protein, membrane-bound lipoprotein (see citation below), equivalent to AL035159|MLCB1450_15|T44736|4154051|CAA22704.1 glutamine-binding protein homolog from Mycobacterium leprae (325 aa), FASTA scores: opt: 1747, E(): 0, (79.3% identity in 328 aa overlap). Also similar to others e.g. GLNH_BACST|P27676 glutamine-binding protein precursor from Bacillus stearothermophilus (262 aa), FASTA scores: opt: 493, E(): 7.5e-22, (37.8% identity in 193 aa overlap); etc. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site, PS01039 Bacterial extracellular solute-binding proteins, family 3 signature. Belongs to the bacterial extracellular solute-binding protein family 3. Presumed attached to the membrane by a lipid anchor. P96257 No cell wall and cell processes ML0303 MMAR_0714 MSMEG_0787 Mb0419c NC_000962.3 Mycobrowser_v2 CDS 498300 499619 . - 0 Rv0412c Rv0412c Unknown Possible conserved membrane protein Rv0412c, (MTCY22G10.08c), len: 439 aa. Possible conserved membrane protein, equivalent to AL035159|MLCB1450_16|T44737 probable membrane protein from Mycobacterium leprae (403 aa), FASTA scores: opt: 2027, E(): 0, (80.4% identity in 403 aa overlap). Also some similarity with CAB71201.1|AL138538 putative secreted protein from Streptomyces coelicolor (429 aa). P96258 No cell wall and cell processes ML0302 MMAR_0715 MSMEG_0788 Mb0420c NC_000962.3 Mycobrowser_v2 RBS 544820 544824 . + 0 Rv0454 Rv0454 Function unknown Conserved hypothetical protein Rv0454, (MTV037.18), len: 116 aa (start uncertain). Conserved hypothetical protein, showing similarity with AAA63007.1|U15183 hypothetical protein from Mycobacterium leprae (115 aa), FASTA scores: opt: 151, E(): 0.0019, (31.5% identity in 89 aa overlap). O86364 No conserved hypotheticals Mb0462 NC_000962.3 Mycobrowser_v2 CDS 544835 545185 . + 0 Rv0454 Rv0454 Function unknown Conserved hypothetical protein Rv0454, (MTV037.18), len: 116 aa (start uncertain). Conserved hypothetical protein, showing similarity with AAA63007.1|U15183 hypothetical protein from Mycobacterium leprae (115 aa), FASTA scores: opt: 151, E(): 0.0019, (31.5% identity in 89 aa overlap). O86364 No conserved hypotheticals Mb0462 NC_000962.3 Mycobrowser_v2 CDS 499713 500366 . + 0 Rv0413 mutT3 Possibly involved in the go system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite DA and DC residues of template DNA with almost equal efficiency thus leading to A.T to G.C transversions. MutT specifically degrades 8-oxo-dGTP to the monophosphate [catalytic activity: 8-oxo-dGTP + H2O = 8-oxo-dGMP + pyrophosphate]. Possible mutator protein MutT3 (7,8-dihydro-8-oxoguanine-triphosphatase) (8-oxo-dGTPase) (dGTP pyrophosphohydrolase) Rv0413, (MTCY22G10.10), len: 217 aa. Possible mutT3, mutator protein (see citation below), showing some similarity with e.g. MUTT_PROVU|P32090 mutator mutt protein from Proteus vulgaris (112 aa), FASTA scores: opt: 151, E(): 0.0008, (40.7% identity in 59 aa overlap). Seems to belong to the NUDIX hydrolase family. P96259 No information pathways ML0301 MMAR_0716 MSMEG_0790 Mb0421 NC_000962.3 Mycobrowser_v2 CDS 500350 501018 . - 0 Rv0414c thiE Involved in thiamine biosynthesis. Condenses 4-methyl-5-(beta-hydroxyethyl)-thiazole monophosphate (THZ-P) and 4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-pp) to form thiamine monophosphate (TMP) [catalytic activity: 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-4-methyl-5-(2-phosphonooxyethyl)-thiazole = diphosphate + thiamine monophosphate]. Thiamine-phosphate pyrophosphorylase ThiE (TMP pyrophosphorylase) (TMP-PPASE) (thiamine-phosphate synthase) Rv0414c, (MTCY22G10.11c), len: 222 aa. thiE, thiamin phosphate pyrophosphorylase, equivalent to Q9ZBL5|AL035159|MLCB1450_17 probable thiamine-phosphate pyrophosphorylase from Mycobacterium leprae (235 aa), FASTA scores: opt: 1095, E(): 0, (78.0% identity in 223 aa overlap). Also similar to others e.g. T34974|5689976|CAB52013.1|AL109663 probable thiamin phosphate pyrophosphorylase from Streptomyces coelicolor (223 aa); THIE_ECOLI|P30137 thie protein from Escherichia coli strain K12 (211 aa), FASTA scores: opt: 275, E(): 7.8e-12, (37.8% identity in 196 aa overlap); etc. Belongs to the TMP-PPASE family. P66916 No intermediary metabolism and respiration ML0300 MMAR_0717 MSMEG_0789 Mb0422c NC_000962.3 Mycobrowser_v2 CDS 501148 502170 . + 0 Rv0415 thiO Possibly involved in thiamine biosynthesis. Possible thiamine biosynthesis oxidoreductase ThiO Rv0415, (MTCY22G10.12), len: 340 aa. Possible thiO, thiamine biosynthesis oxidoreductase, equivalent to T44739|4154054|CAA22708.1|AL035159|MLCB1450.24 hypothetical protein from Mycobacterium leprae (340 aa), FASTA scores: opt: 1867, E(): 0, (82.0% identity in 338 aa overlap). Shows some similarity to other thiO proteins e.g. THIO_RHIET|O34292 Putative thiamine biosynthesis oxidoreductase from Rhizobium etli plasmid pb (327 aa) (see citation below); AAG31046.1|AF264948_8|THIO putative amino acid oxidase flavoprotein ThiO from Erwinia amylovora (349 aa); NP_106392.1|14025578|BAB52178.1|AP003007|THIO thiamine biosynthesis oxidoreductase THIO from Mesorhizobium loti (333 aa); etc. P96261 No intermediary metabolism and respiration ML0299 MMAR_0718 MSMEG_0791 Mb0423 NC_000962.3 Mycobrowser_v2 CDS 502167 502373 . + 0 Rv0416 thiS Possibly involved in thiamine biosynthesis. Possible protein ThiS Rv0416, (MTCY22G10.13), len: 68 aa. Possible thiS protein, equivalent to T44740|4154055|CAA22709.1|AL035159|MLCB1450.25 hypothetical protein from Mycobacterium leprae (74 aa), FASTA scores: opt: 303, E(): 2e-18, (71.6% identity in 74 aa overlap). Shows weak similarity with O32583|THIS_ECOLI|THIG1|B3991.1 this protein from Escherichia coli strain K12 (66 aa), FASTA scores: opt: 103, E(): 0.052, (30.9% identity in 68 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P96262 No intermediary metabolism and respiration ML0298 MMAR_0719 MSMEG_0792 Mb0424 NC_000962.3 Mycobrowser_v2 CDS 1027104 1027685 . + 0 Rv0921 Rv0921 Prevents the cointegration of foreign DNA before integration into the chromosome. Possible resolvase Rv0921, (MTCY21C12.15), len: 193 aa. Possible resolvase for IS1535, highly similar to many bacterial resolvases e.g. MTCY274.17c|YX1C_MYCTU Q10831 from Mycobacterium tuberculosis (295 aa), FASTA scores: opt: 537, E(): 5.7e-29, (51.8% identity in 166 aa overlap). Presents an helix turn helix motif. O05913 No insertion seqs and phages Mb0945 NC_000962.3 Mycobrowser_v2 CDS 502366 503124 . + 0 Rv0417 thiG Involved in thiamine biosynthesis. Required for the synthesis of the thiazole moiety of thiamine. Probable thiamin biosynthesis protein ThiG (thiazole biosynthesis protein) Rv0417, (MTCY22G10.14), len: 252 aa. Probable thiG, thiamin biosynthesis protein, equivalent to AL035159|MLCB1450_20|T44741|THIG probable thiamin biosynthesis protein from Mycobacterium leprae (261 aa), FASTA scores: opt: 1380, E(): 0, (86.8% identity in 250 aa overlap). Also highly similar to others e.g. SCOEDB|SC6E10.03|T35490|THIG probable thiazole biosynthesis protein from Streptomyces coelicolor (264 aa); F82761|9105679|AAF83593.1|AE003919_4|XF0783|THIG thiamin biosynthesis protein thiG from Xylella fastidiosa (275 aa); P30139|THIG_ECOLI|7448315|B65206|409790|AAC43089.1|U00006 THIG protein thiamin biosynthesis protein from Escherichia coli strain K-12 (281 aa); etc. Belongs to the THIG family. P96263 No intermediary metabolism and respiration ML0297 MMAR_0720 MSMEG_0793 Mb0425 NC_000962.3 Mycobrowser_v2 CDS 545889 546803 . - 0 Rv0456c echA2 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. enoyl-CoA hydratase EchA2 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv0456c, (MTCI429A.02, MTV037.20c), len: 304 aa. Probable echA2, enoyl-CoA hydratase, similar to other enoyl-CoA hydratases e.g. Q13011 peroxisomal enoyl-CoA hydratase-like protein (328 aa), FASTA scores: opt: 209, E(): 5.3e-07, (31.7% identity in 142 aa overlap). Also similar to several other proteins from Mycobacterium tuberculosis e.g. MTCY09F9.29 FASTA score: (32.9% identity in 146 aa overlap); and MTI376.01c. O07179 No lipid metabolism ML2381 MMAR_0778 Mb0464c NC_000962.3 Mycobrowser_v2 CDS 503496 504998 . + 0 Rv0418 lpqL Function unknown; hydrolyzes peptides and/or proteins. Probable lipoprotein aminopeptidase LpqL Rv0418, (MTCCY22G10.15), len: 500 aa. Probable lpqL, lipoprotein aminopeptidase, similar to others e.g. B83278|9949035|AAG06327.1|AE004720_3|AE004720|PA2939 probable aminopeptidase from Pseudomonas aeruginosa (536 aa); P80561|APX_STRGR|SGAP|S66427 aminopeptidase from Streptomyces griseus (284 aa) (homology only with C-terminus of Rv0418); P37302|APE3_YEAST|1077010|A54134 aminopeptidase Y from Saccharomyces cerevisiae (537 aa); etc. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. P96264 No cell wall and cell processes MMAR_0726 MSMEG_0806 Mb0426 NC_000962.3 Mycobrowser_v2 CDS 505086 506582 . + 0 Rv0419 lpqM Function unknown; possibly hydrolyzes peptides and/or proteins. Possible lipoprotein peptidase LpqM Rv0419, (MTCY22G10.16), len: 498 aa. Possible lpqM, lipoprotein peptidase ; has potential N-terminal signal peptide and contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site, PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. P96265 No cell wall and cell processes MMAR_0728 MSMEG_4893,MSMEG_4913 NC_000962.3 Mycobrowser_v2 CDS 506561 506971 . - 0 Rv0420c Rv0420c Unknown Possible transmembrane protein Rv0420c, (MTCY22G10.17c), len: 136 aa. Possible transmembrane protein; has potential transmembrane domains aa 53-99 and aa 100-122. P96266 No cell wall and cell processes MMAR_0731 MSMEG_0813 Mb0428c NC_000962.3 Mycobrowser_v2 CDS 507132 507761 . - 0 Rv0421c Rv0421c Function unknown Conserved hypothetical protein Rv0421c, (MTCY22G10.18c), len: 209 aa. Conserved hypothetical protein, showing similarity with NP_103507.1|14022684|BAB49293.1|AP002998 hypothetical protein from Mesorhizobium loti (214 aa). P96267 No conserved hypotheticals ML0296 MMAR_0733 MSMEG_0824 Mb0429c NC_000962.3 Mycobrowser_v2 CDS 507758 508555 . - 0 Rv0422c thiD Involved in thiamine biosynthesis. Catalyzes the phosphorylation of HMP-P to HMP-pp [catalytic activity: ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine]. Probable phosphomethylpyrimidine kinase ThiD (HMP-phosphate kinase) (HMP-P kinase) Rv0422c, (MTCY22G10.19c), len: 265 aa. Probable thiD, phosphomethylpyrimidine kinase, equivalent to AL035159|MLCB1450_21 phosphomethylpyrimidine kinase from Mycobacterium leprae (279 aa), FASTA scores: opt: 1386, E(): 0, (77.8% identity in 266 aa overlap). Also highly similar to others e.g. HIU32725_3|P44697|THID_HAEIN phosphomethylpyrimidine kinase from Haemophilus influenzae (269 aa), FASTA scores: opt: 605, E(): 0, (42.1% identity in 259 aa overlap). Belongs to the ThiD family. P66913 No intermediary metabolism and respiration ML0295 MMAR_0734 MSMEG_0825 Mb0430c NC_000962.3 Mycobrowser_v2 CDS 508582 510225 . - 0 Rv0423c thiC Involved in thiamine biosynthesis. Required for the synthesis of the hydromethylpyrimidine (HMP) moiety of thiamine (4-amino-2-methyl-5-hydroxymethylpyrimidine). Probable thiamine biosynthesis protein ThiC Rv0423c, (MTCY22G10.20c), len: 547 aa. Probable thiC, thiamin biosynthesis protein, equivalent to Q9ZBL0|THIC_MYCLE|11279601|T44743|AL035159|MLCB1450_22 thiamine biosynthesis protein from Mycobacterium leprae (547 aa), FASTA scores: opt: 3283, E(): 0, (90.1% identity in 547 aa overlap). Also highly similar to others e.g. P45740|THIC_BACSU thiamin biosynthesis protein from Bacillus subtilis (590 aa), FASTA scores: opt: 2295, E(): 0, (65.2% identity in 580 aa overlap); P30136|THIC_ECOLI THIC protein from Escherichia coli strain K12 (631 aa), FASTA scores: opt: 2141, E(): 0, (62.1% identity in 568 aa overlap); etc. Belongs to the ThiC family. P66911 No intermediary metabolism and respiration ML0294 MMAR_0735 MSMEG_0826 Mb0431c NC_000962.3 Mycobrowser_v2 CDS 510377 510652 . - 0 Rv0424c Rv0424c Unknown Hypothetical protein Rv0424c, (MTCY22G10.21c), len: 91 aa. Hypothetical unknown protein. P96270 No conserved hypotheticals ML1934 Mb0432c NC_000962.3 Mycobrowser_v2 CDS 510702 515321 . - 0 Rv0425c ctpH Metal cation-transporting ATPase; possibly catalyzes the transport of an undetermined metal cation with the hydrolysis of ATP [catalytic activity: ATP + H(2)O + undetermined metal cation(in) = ADP + phosphate + undetermined metal cation(out)]. Possible metal cation transporting P-type ATPase CtpH Rv0425c, (MTCY22G10.22c), len: 1539 aa. Possible ctpH, metal cation-transporting P-type ATPase (transmembrane protein), showing some similarity with CAA17934.1|AL022118|13093871|CAC32203.1|AL583926 putative cation-transporting ATPase from Mycobacterium leprae (1609 aa). Also similar to others ATPases e.g. AE000873_1 cation-transporting P-ATPase from Methanobacterium thermoautotrop (844 aa), FASTA score: (30.5% identity in 827 aa overlap); AB69720.1|AL137166 putative transport ATPase from Streptomyces coelicolor (1472 aa); etc. C-terminal region similar to other ATPases from Mycobacterium tuberculosis e.g. Y05Q_MYCTU|Q10900 putative cation-transporting ATPase C (855 aa), FASTA scores: opt: 770, E(): 5.3e-32, (44.9% identity in 820 aa overlap). Nucleotide position 511518 in the genome sequence has been corrected, T:G resulting in I1268I. P96271 No cell wall and cell processes ML1933 MMAR_0740 Mb0433c NC_000962.3 Mycobrowser_v2 CDS 515373 515816 . - 0 Rv0426c Rv0426c Unknown Possible transmembrane protein Rv0426c, (MTCY22G10.23c), len: 147 aa. Possible transmembrane protein; has potential transmembrane domains aa 19-41, and aa 61-83. P96272 No cell wall and cell processes ML1932 Mb0434c NC_000962.3 Mycobrowser_v2 CDS 516017 516892 . - 0 Rv0427c xthA Involved in base excision repair. Apurinic-apyrimidinic endonuclease. Supposed to remove the damaged DNA at cytosines and guanines by cleaving at the 3' side of the AP site by a beta-elimination reaction. Possibly exhibites 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase and ribonuclease H activities [catalytic activity: degradation of double-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing 5'-phosphomononucleotides]. Probable exodeoxyribonuclease III protein XthA (exonuclease III) (EXO III) (AP endonuclease VI) Rv0427c, (MTCY22G10.24c), len: 291 aa. Probable xthA (alternate gene name: xth), exodeoxyribonuclease III protein (see citation below), similar to others e.g. EX3_ECOLI|P09030 exodeoxyribonuclease III from Escherichia Coli strain K12 (268 aa), FASTA scores: opt: 360, E(): 1.2e-17, (29.3% identity in 270 aa overlap); etc. Belongs to the AP/EXOA family of DNA repair enzymes. P96273 No information pathways ML1931 MMAR_0742 MSMEG_0829 Mb0435c NC_000962.3 Mycobrowser_v2 CDS 517803 518396 . - 0 Rv0429c def Removes the formyl group from the N-terminal met of newly synthesized proteins [catalytic activity: N-formyl-L-methionine + H2O = formate + L-methionine]. Probable polypeptide deformylase Def (PDF) (formylmethionine deformylase) Rv0429c, (MTCY22G10.26c), len: 197 aa. Probable def, polypeptide deformylase, equivalent to CAC30884.1|AL583923 polypeptide deformylase from Mycobacterium leprae (197 aa). Also similar to others e.g. DEF_ECOLI|P27251|95874|S23107 polypeptide deformylase from Escherichia coli (169 aa), FASTA scores: opt: 179, E(): 1.8e-05, (34.6% identity in 162 aa overlap); etc. Belongs to the polypeptide deformylase family. Cofactor: binds 1 zinc ion. P96275 No information pathways ML1929 MMAR_0744 MSMEG_0832 Mb0437c NC_000962.3 Mycobrowser_v2 CDS 518733 519041 . + 0 Rv0430 Rv0430 Function unknown Conserved hypothetical protein Rv0430, (MTCY22G10.27), len: 102 aa. Conserved hypothetical protein, equivalent to AC30882.1|AL583923 conserved hypothetical protein from Mycobacterium leprae (102 aa). Also highly similar to CAB93047.1|SCD95A.20|AL357432 hypothetical protein from Streptomyces coelicolor (84 aa). P96276 No conserved hypotheticals ML1927 Mb0438 NC_000962.3 Mycobrowser_v2 CDS 519073 519567 . + 0 Rv0431 Rv0431 Unknown Putative tuberculin related peptide Rv0431, (MTCY22G10.28), len: 164 aa. Putative tuberculin related peptide; almost identical to D00815|MSGAT103_1 AT103 from Mycobacterium tuberculosis (172 aa), FASTA score: (99.4% identity in 163 aa overlap). Highly similar to to CAC30881.1|AL583923 tuberculin related peptide (AT103) from Mycobacterium leprae (167 aa). Some similarity to G550415|HRPC (282 aa), FASTA scores: opt: 120, E(): 0.36, (33.3% identity in 111 aa overlap). Potential transmembrane domain at aa 19-37. P96277 No cell wall and cell processes ML1926 MMAR_0746 MSMEG_0834 Mb0439 NC_000962.3 Mycobrowser_v2 CDS 519600 520322 . + 0 Rv0432 sodC Destroys radicals which are normally produced within the cells and are toxic to biological systems [catalytic activity: 2 superoxide + 2 H+ = O2 + H2O2]. Periplasmic superoxide dismutase [Cu-Zn] SodC Rv0432, (MTCY22G10.29), len: 240 aa. sodC, periplasmic superoxide dismutase [Cu-Zn], equivalent to CAC30880.1|AL583923 superoxide dismutase precursor (Cu-Zn) from Mycobacterium leprae (240 aa); and AAK20038.1|AF326234_1 copper zinc superoxide dismutase from Mycobacterium avium subsp. paratuberculosis (226 aa). Also similar to others e.g. SODC_PHOLE|P00446 superoxide dismutase precursor (Cu-Zn) from Photobacterium leiognathi (173 aa), FASTA scores: opt: 214, E(): 5.2 e-06, (36.5% identity in 181 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Belongs to the Cu-Zn superoxide dismutase family. Possibly localized in periplasm, membrane-bound. P0A608 No virulence, detoxification, adaptation ML1925 MMAR_0747 MSMEG_0835 Mb0440 NC_000962.3 Mycobrowser_v2 CDS 2370598 2370792 . - 0 Rv2111c pup Involved in proteasomal degradation. Covalently binds to protein substrates. Prokaryotic ubiquitin-like protein Pup Rv2111c, MTCY261.07c, len: 64 aa. Pup, prokaryotic ubiquitin-like protein (See Pearce et al., 2008). Highly similar to many. Pup|Rv2111c and Mpa|Rv2115c interact (See Pearce et al., 2008). O33246 No intermediary metabolism and respiration ML1321 MMAR_3086 MSMEG_3896 Mb2135c NC_000962.3 Mycobrowser_v2 RBS 2370798 2370803 . - 0 Rv2111c pup Involved in proteasomal degradation. Covalently binds to protein substrates. Prokaryotic ubiquitin-like protein Pup Rv2111c, MTCY261.07c, len: 64 aa. Pup, prokaryotic ubiquitin-like protein (See Pearce et al., 2008). Highly similar to many. Pup|Rv2111c and Mpa|Rv2115c interact (See Pearce et al., 2008). O33246 No intermediary metabolism and respiration ML1321 MMAR_3086 MSMEG_3896 Mb2135c NC_000962.3 Mycobrowser_v2 CDS 521514 522167 . + 0 Rv0434 Rv0434 Function unknown Conserved hypothetical protein Rv0434, (MTCY22G10.31), len: 217 aa. Conserved hypothetical protein, similar to AE002052_2 from Deinococcus radiodurans (213 aa), FASTA scores: opt: 258, E(): 4e-10, (31.9% identity in 213 aa overlap); SYCSLRB_122|Q55701 hypothetical 24.5 kDa protein from Synechocystis (214 aa), FASTA scores: opt: 156, E(): 0.00041, (28.4% identity in 204 aa overlap); MXABSGA_1|LON2_MYXXA|P36774 ATP-dependent protease la 2 from Myxococcus xanthus (826 aa), FASTA scores: opt: 160, E(): 0.00068, (28.4% identity in 197 aa overlap); etc. P96280 No conserved hypotheticals ML0307 Mb0442 NC_000962.3 Mycobrowser_v2 CDS 554313 554885 . - 0 Rv0464c Rv0464c Function unknown Conserved protein Rv0464c, (MTV038.08c), len: 190 aa. Conserved protein, highly similar to CAC31982.1|AL583925 conserved hypothetical protein from Mycobacterium leprae (188 aa). Also some similarity with Rv1531|AL022000|MTV045_5|D70820 hypothetical protein from Mycobacterium tuberculosis (188 aa), FASTA scores: E(): 9.6e-10, (30.9% identity in 175 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53749 No conserved hypotheticals ML2465 MMAR_0789 MSMEG_0905 Mb0473c NC_000962.3 Mycobrowser_v2 CDS 522347 524533 . - 0 Rv0435c Rv0435c ATPase; possibly catalyzes the transport of undetermined substrate with hydrolyse of ATP [catalytic activity: ATP + H(2)O + undetermined substrate(in) = ADP + phosphate + undetermined substrate(out)]. Putative conserved ATPase Rv0435c, (MTCY22G10.32c), len: 728 aa. Putative conserved ATPase, similar to others e.g. SAV_SULAC|Q07590 sav protein involved in cell division from sulfolobus acidocaldarius (780 aa), FASTA scores: opt: 897, E(): 0, (34.5% identity in 693 aa overlap); NP_148637.1|7435761|B72479 transitional endoplasmic reticulum ATPase from Aeropyrum pernix (699 aa); etc. Also similar to Rv3610c and Rv2115c from Mycobacterium tuberculosis. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00674 AAA-protein family signature. P96281 No cell wall and cell processes ML0309 MMAR_0752 MSMEG_0858 Mb0443c NC_000962.3 Mycobrowser_v2 CDS 524530 525390 . - 0 Rv0436c pssA Involved in phospholipid biosynthesis. Generates phosphatidylserine [catalytic activity: CDP-diacylglycerol + L-serine = CMP + O-SN-phosphatidyl-L-serine]. Probable CDP-diacylglycerol--serine O-phosphatidyltransferase PssA (PS synthase) (phosphatidylserine synthase) Rv0436c, (MTCY22G10.33c), len: 286 aa. Probable pssA, PS synthase (CDP-diacylglycerol--serine O-phosphatidyltransferase) (see citation below), integral membrane protein, equivalent to AL035159|MLCB1450_9|T44730 from Mycobacterium leprae (300 aa), FASTA scores: opt: 1506, E(): 0, (77.9% identity in 285 aa overlap). Also highly similar to others e.g. NP_108059.1|14027250|BAB54204.1|AP003012 phosphatidylserine synthase from Mesorhizobium loti (248 aa); PSS_BACSU|P39823 cdp-diacylglycerol--serine o-phosphatidyltransferase from Bacillus subtilis (177 aa), FASTA scores: opt: 277, E(): 9.9e-12, (33.3% identity in 183 aa overlap); etc. Contains PS00379 CDP-alcohol phosphatidyltransferases signature. Belongs to the CDP-alcohol phosphatidyltransferase class-I family. P96282 No lipid metabolism ML0310 MMAR_0753 MSMEG_0860 Mb0444c NC_000962.3 Mycobrowser_v2 CDS 525387 526082 . - 0 Rv0437c psd Function unknown, but involved in lipid metabolism [catalytic activity: phosphatidyl-L-serine = phosphatidylethanolamine + CO(2)]. Possible phosphatidylserine decarboxylase Psd (PS decarboxylase) Rv0437c, (MTV037.01c), len: 231 aa (start uncertain). Possible psd, phosphatidylserine decarboxylase , equivalent to CAC29819.1|AL583918 conserved hypothetical protein from Mycobacterium leprae (243 aa); and highly similar to MLCB1450.11|T44729|4154044|CAA22695.1|AL035159 hypothetical protein from Mycobacterium leprae (202 aa), FASTA score: (74.6% identity in 197 aa overlap). Also similar to other phosphatidylserine decarboxylases e.g. NP_108058.1|14027249|BAB54203.1|AP003012 phosphatidylserine decarboxylase from Mesorhizobium loti (232 aa); AAK86872|g15156090|AGR_C_1963 phosphatidylserine decarboxylase from Agrobacterium tumefaciens (244 aa); AAG12422.1|AY005137|Psd phosphatidylserine decarboxylase from Chlorobium tepidum (216 aa); etc. P67547 No lipid metabolism ML0311 MMAR_0754 MSMEG_0861 Mb0445c NC_000962.3 Mycobrowser_v2 RBS 526091 526094 . - 0 Rv0437c psd Function unknown, but involved in lipid metabolism [catalytic activity: phosphatidyl-L-serine = phosphatidylethanolamine + CO(2)]. Possible phosphatidylserine decarboxylase Psd (PS decarboxylase) Rv0437c, (MTV037.01c), len: 231 aa (start uncertain). Possible psd, phosphatidylserine decarboxylase , equivalent to CAC29819.1|AL583918 conserved hypothetical protein from Mycobacterium leprae (243 aa); and highly similar to MLCB1450.11|T44729|4154044|CAA22695.1|AL035159 hypothetical protein from Mycobacterium leprae (202 aa), FASTA score: (74.6% identity in 197 aa overlap). Also similar to other phosphatidylserine decarboxylases e.g. NP_108058.1|14027249|BAB54203.1|AP003012 phosphatidylserine decarboxylase from Mesorhizobium loti (232 aa); AAK86872|g15156090|AGR_C_1963 phosphatidylserine decarboxylase from Agrobacterium tumefaciens (244 aa); AAG12422.1|AY005137|Psd phosphatidylserine decarboxylase from Chlorobium tepidum (216 aa); etc. P67547 No lipid metabolism ML0311 MMAR_0754 MSMEG_0861 Mb0445c NC_000962.3 Mycobrowser_v2 CDS 526143 527360 . - 0 Rv0438c moeA2 Involved in molybdenum cofactor biosynthesis: involved in the biosynthesis of a demolybdo-cofactor (molybdopterin), necessary for molybdo-enzymes. Probable molybdopterin biosynthesis protein MoeA2 Rv0438c, (MTV037.02c), len: 405 aa. Probable moeA2, molybdenum cofactor biosynthesis protein, highly similar to many e.g. Y10817|ANY10817_2 from A. nicotinovorans (429 aa), FASTA scores: opt: 786, E(): 0, (39.2% identity in 398 aa overlap); etc. Also similar to MOEA1|Rv0994|MTCI237.08|O05577 probable molybdopterin biosynthesis protein from Mycobacterium tuberculosis (426 aa), FASTA scores: opt: 667, E(): 2e-32, (36.5% identity in 425 aa overlap). Note that previously known as moeA3. O53725 No intermediary metabolism and respiration ML0312 MMAR_0756 MSMEG_0862 Mb0446c NC_000962.3 Mycobrowser_v2 RBS 3477643 3477646 . + 0 Rv0438c moeA2 Involved in molybdenum cofactor biosynthesis: involved in the biosynthesis of a demolybdo-cofactor (molybdopterin), necessary for molybdo-enzymes. Probable molybdopterin biosynthesis protein MoeA2 Rv0438c, (MTV037.02c), len: 405 aa. Probable moeA2, molybdenum cofactor biosynthesis protein, highly similar to many e.g. Y10817|ANY10817_2 from A. nicotinovorans (429 aa), FASTA scores: opt: 786, E(): 0, (39.2% identity in 398 aa overlap); etc. Also similar to MOEA1|Rv0994|MTCI237.08|O05577 probable molybdopterin biosynthesis protein from Mycobacterium tuberculosis (426 aa), FASTA scores: opt: 667, E(): 2e-32, (36.5% identity in 425 aa overlap). Note that previously known as moeA3. O53725 No intermediary metabolism and respiration ML0312 MMAR_0756 MSMEG_0862 Mb0446c NC_000962.3 Mycobrowser_v2 CDS 527379 528314 . - 0 Rv0439c Rv0439c Function unknown; possibly involved in cellular metabolism. Probable dehydrogenase/reductase Rv0439c, (MTV037.03c), len: 311 aa. Probable dehydrogenase/reductase, equivalent to AL035159|MLCB1450_6|T44727 probable oxidoreductase from Mycobacterium leprae (304 aa), FASTA scores: opt: 1360, E(): 0, (69.2% identity in 302 aa overlap). Also highly similar to various oxidoreductases, generally dehydrogenases/reductases e.g. PA5031|C83017|9951320|AAG08416.1|AE004916_5|AE004916 probable short chain dehydrogenase from Pseudomonas aeruginosa (309 aa); Q03326|OXIR_STRAT probable oxidoreductase from Streptomyces antibioticus (298 aa), FASTA scores: opt: 400, E(): 1.2e-18, (34.6% identity in 298 aa overlap); etc. O53726 No intermediary metabolism and respiration ML0313 Mb0447c NC_000962.3 Mycobrowser_v2 CDS 547076 547357 . - 0 Rv0456A mazF1 Function unknown Possible toxin MazF1 Rv0456A, len: 93 aa. Possible mazF1, toxin, part of toxin-antitoxin (TA) operon with Rv0456B (See Pandey and Gerdes, 2005; Zhu et al., 2006); N-terminus highly similar to N-terminal part of P71650|Rv2801c|MT2869|MTCY16B7.42 conserved hypothetical protein from Mycobacterium tuberculosis (118 aa), FASTA scores: opt: 303, E(): 1e-14, (60.44% identity in 91 aa overlap). Also some similarity in part with other hypothetical proteins e.g. Q9PHH8|XFA0027 Plasmid maintenance protein from Xylella fastidiosa (108 aa), FASTA scores: opt: 169, E(): 3.9e-05, (50.820% identity in 61 aa overlap). Q6MX40 No virulence, detoxification, adaptation Mb0465c NC_000962.3 Mycobrowser_v2 RBS 528591 528596 . + 0 Rv0440 groEL2 Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions. 60 kDa chaperonin 2 GroEL2 (protein CPN60-2) (GroEL protein 2) (65 kDa antigen) (heat shock protein 65) (cell wall protein A) (antigen A) Rv0440, (MTV037.04), len: 540 aa. GroEL2 (alternate gene names: groL2, groEL-2, hsp65, hsp60), 60 kDa chaperonin 2 (see Shinnick 1987). Purified 65 kDa antigen can elicit a strong delayed-type hypersensitivity reaction in experimental animals infected with M. tuberculosis. This protein is one of the major immunoreactive proteins of the mycobacteria. This antigen contains epitopes that are common to various species of mycobacteria. Contains PS00296 Chaperonins cpn60 signature. Belongs to the chaperonin (HSP60) family. Phosphorylated in vitro by PknJ|Rv2088 (See Arora et al., 2010). P0A520 No virulence, detoxification, adaptation ML0317 MMAR_0759 Mb0448 NC_000962.3 Mycobrowser_v2 CDS 528608 530230 . + 0 Rv0440 groEL2 Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions. 60 kDa chaperonin 2 GroEL2 (protein CPN60-2) (GroEL protein 2) (65 kDa antigen) (heat shock protein 65) (cell wall protein A) (antigen A) Rv0440, (MTV037.04), len: 540 aa. GroEL2 (alternate gene names: groL2, groEL-2, hsp65, hsp60), 60 kDa chaperonin 2 (see Shinnick 1987). Purified 65 kDa antigen can elicit a strong delayed-type hypersensitivity reaction in experimental animals infected with M. tuberculosis. This protein is one of the major immunoreactive proteins of the mycobacteria. This antigen contains epitopes that are common to various species of mycobacteria. Contains PS00296 Chaperonins cpn60 signature. Belongs to the chaperonin (HSP60) family. Phosphorylated in vitro by PknJ|Rv2088 (See Arora et al., 2010). P0A520 No virulence, detoxification, adaptation ML0317 MMAR_0759 Mb0448 NC_000962.3 Mycobrowser_v2 CDS 530296 530724 . - 0 Rv0441c Rv0441c Unknown Hypothetical protein Rv0441c, (MTV037.05c), len: 142 aa. Hypothetical unknown protein. P0A5C7 No conserved hypotheticals MMAR_0760 MSMEG_0882 Mb0449c NC_000962.3 Mycobrowser_v2 CDS 530751 532214 . - 0 Rv0442c PPE10 Function unknown PPE family protein PPE10 Rv0442c, (MTV037.06c), len: 487 aa. PPE10, Member of the Mycobacterium tuberculosis PPE family, nearly identical to hypothetical protein from Mycobacterium tuberculosis (strain Erdman) and to AN5S46909_1 protein fragment from Mycobacterium bovis (302 aa); P42611|YHS6_MYCTU hypothetical 50.6 kDa protein (517 aa), FASTA scores: opt: 3144, E(): 0, (98.4 identity in 492 aa overlap); and S46909|S46909_1 (302 aa), FASTA scores: opt: 1897, E(): 0, (98.0% identity in 302 aa overlap). Nucleotide position 532097 in the genome sequence has been corrected, T:C resulting in K40E. P42611 No PE/PPE ML2369c Mb0450c NC_000962.3 Mycobrowser_v2 CDS 533091 533789 . - 0 Rv0444c rskA Regulates negatively Rv0445c|SIGK Anti-sigma factor RskA (regulator of sigma K) Rv0444c, (MTV037.08c), len: 232 aa. RskA, regulator of SigK (See Said-Salim et al., 2006); C-terminus similar to P12752|Y24K_STRGR hypothetical 24.7 kDa protein from Streptomyces griseus (238 aa), FASTA scores: opt: 207, E(): 2.2e-05, (32.9% identity in 158 aa overlap). Cleaved by Rip|Rv2869c, in M. tuberculosis Erdman (See Sklar et al., 2010). O53729 No information pathways MMAR_0765 Mb0452c NC_000962.3 Mycobrowser_v2 RBS 533800 533803 . - 0 Rv0444c rskA Regulates negatively Rv0445c|SIGK Anti-sigma factor RskA (regulator of sigma K) Rv0444c, (MTV037.08c), len: 232 aa. RskA, regulator of SigK (See Said-Salim et al., 2006); C-terminus similar to P12752|Y24K_STRGR hypothetical 24.7 kDa protein from Streptomyces griseus (238 aa), FASTA scores: opt: 207, E(): 2.2e-05, (32.9% identity in 158 aa overlap). Cleaved by Rip|Rv2869c, in M. tuberculosis Erdman (See Sklar et al., 2010). O53729 No information pathways MMAR_0765 Mb0452c NC_000962.3 Mycobrowser_v2 CDS 533833 534396 . - 0 Rv0445c sigK Involved in transcription mechanism. The sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released. Alternative RNA polymerase sigma factor SigK Rv0445c, (MTV037.09c), len: 187 aa. sigK, alternative RNA polymerase sigma factor (see citations below), highly similar to others e.g. 5531433|CAB50938.1|AL096849|T36745 probable RNA polymerase sigma factor from Streptomyces coelicolor (185 aa); NP_105607.1|14024791|BAB51393.1|AP003005 RNA polymerase sigma factor from Mesorhizobium loti (179 aa); 1654108|AAB17906.1|U11283|A58883 probable transcription initiation factor sigma E from Rhodobacter phaeroides (168 aa), FASTA scores: opt: 299, E(): 2e-14, (32.7% identity in 168 aa overlap); Q45585|SIGW_BACSU RNA polymerase sigma factor SIGW from Bacillus subtilis (187 aa), FASTA scores: opt: 213, E(): 2.9e-08, (26.8% identity in 179 aa overlap); etc. O53730 No information pathways ML2370 MMAR_0766 Mb0453c NC_000962.3 Mycobrowser_v2 CDS 534445 535215 . - 0 Rv0446c Rv0446c Unknown Possible conserved transmembrane protein Rv0446c, (MTV037.10c), len: 256 aa. Possible conserved transmembrane protein, similar at N-terminus to U1740AF|U15183|MLU15183_40 from Mycobacterium leprae (117 aa), FASTA scores: opt: 175, E(): 2.5e-05, (62.5% identity in 40 aa overlap); and at C-terminus to AL021529|SC10A5_3 from Streptomyces coelicolor (226 aa), FASTA scores: opt: 207, E(): 9.8e-07, (34.2% identity in 114 aa overlap). Also similar to others hypothetical proteins e.g. AAK04680.1|AE006291_14|AE006291 hypothetical protein from Lactococcus lactis subsp. lactis (257 aa). O53731 No cell wall and cell processes ML2371 MMAR_0767 Mb0454c NC_000962.3 Mycobrowser_v2 CDS 543174 544730 . + 0 Rv0453 PPE11 Function unknown PPE family protein PPE11 Rv0453, (MTV037.17), len: 518 aa. PPE11, Member of the Mycobacterium tuberculosis PPE family, similar to many e.g. AL0212|MTV012_32 from Mycobacterium tuberculosis (434 aa), FASTA scores: opt: 882, E(): 7e-31, (41.8% identity in 514 aa overlap). Q6MX41 No PE/PPE Mb0461 NC_000962.3 Mycobrowser_v2 CDS 535224 536507 . - 0 Rv0447c ufaA1 Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge [catalytic activity: S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid]. Probable cyclopropane-fatty-acyl-phospholipid synthase UfaA1 (cyclopropane fatty acid synthase) (CFA synthase) Rv0447c, (MTV037.11c), len: 427 aa (start uncertain). Probable ufaA1, cyclopropane-fatty-acyl-phospholipid synthase, similar to others e.g. NP_102178.1|14021351|BAB47964.1|AP002994 cyclopropane-fatty-acyl-phospholipid synthase from Mesorhizobium loti (378 aa); B82240|9655593|AAF94281.1|AE004192 cyclopropane-fatty-acyl-phospholipid synthase from Vibrio cholerae (432 aa); P30010|CFA_ECOLI cyclopropane-fatty-acyl-phospholipid synthase from Escherichia coli strain K-12 (382 aa); X55704|PPLPD_3 LPD-3 from P.putida (394 aa), FASTA scores: opt: 556, E(): 2.8e-30, (33.3% identity in 387 aa overlap); AE0005|HPAE000557_9 from Helicobacter pylori (389 aa), FASTA scores: opt: 539, E(): 3.9e-29, (34.3% identity in 382 aa overlap). O53732 No lipid metabolism ML2372 Mb0455c NC_000962.3 Mycobrowser_v2 CDS 536504 537169 . - 0 Rv0448c Rv0448c Function unknown Conserved hypothetical protein Rv0448c, (MTV037.12c), len: 221 aa. Conserved hypothetical protein, similar to other hypothetical proteins e.g. Z74841|BOD5A2_1 from B. oleracea (283 aa), FASTA scores: opt: 257, E(): 1.4e-10, (32.0% identity in 197 aa overlap); etc. Some similarity to U15183|MLU15183_38 from Mycobacterium leprae (82 aa), FASTA scores: opt: 134, E(): 0.014, (71.0% identity in 31 aa overlap). O53733 No conserved hypotheticals ML2373 MMAR_0769 Mb0456c NC_000962.3 Mycobrowser_v2 CDS 537229 538548 . - 0 Rv0449c Rv0449c Function unknown Conserved hypothetical protein Rv0449c, (MTV037.13c), len: 439 aa. Conserved hypothetical protein, some similarity with several hypothetical proteins and various enzymes e.g. AAK24569.1|AE005927 amine oxidase, flavin-containing from Caulobacter crescentus (454 aa); BAB02771.1|AB023036 mycolic acid methyl transferase-like protein from Arabidopsis thaliana (842 aa); BAB01742.1|AP000374 protein which contains similarity to cyclopropane fatty acid synthase from Arabidopsis thaliana (793 aa); etc. Has hydrophobic stretch at N-terminus. O53734 No conserved hypotheticals MMAR_0770 Mb0457c NC_000962.3 Mycobrowser_v2 CDS 538588 541491 . - 0 Rv0450c mmpL4 Unknown. Thought to be involved in fatty acid transport. Probable conserved transmembrane transport protein MmpL4 Rv0450c, (MTV037.14c), len: 967 aa. Probable mmpL4, conserved transmembrane transport protein (see citations below), member of RND superfamily, equivalent to U1740V|P54881|YV34_MYCLE hypothetical 105.2 kDa protein from Mycobacterium leprae (959 aa), FASTA scores: opt: 5051, E(): 0, (78.4% identity in 962 aa overlap). Also highly similar to other proteins from Mycobacterium tuberculosis e.g. Z83860|MTCY98.08 (962 aa), FASTA scores: opt: 3917, E(): 0, (61.3% identity in 950 aa overlap), MTCY20G9.34, etc. Contains PS00211 ABC transporters family signature. Belongs to the MmpL family. O53735 No cell wall and cell processes ML2378 MMAR_0771 MSMEG_0382 Mb0458c NC_000962.3 Mycobrowser_v2 CDS 541488 541910 . - 0 Rv0451c mmpS4 Unknown Probable conserved membrane protein MmpS4 Rv0451c, (MTV037.15c), len: 140 aa. Probable mmpS4, conserved membrane protein (see citations below), equivalent to U1740W|P54880|YV33_MYCLE hypothetical 16.9 kDa protein from Mycobacterium leprae (154 aa), FASTA scores: opt: 727, E(): 0, (75.9% identity in 137 aa overlap). Also similar to other Mycobacterial proteins e.g. Z84725|MTCY04D9.16c from Mycobacterium tuberculosis (142 aa), FASTA scores: opt: 451, E(): 3.2e-24, (50.0% identity in 138 aa overlap); etc. Belongs to the MmpS family. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). P0A5K2 No cell wall and cell processes ML2377 MMAR_0772 MSMEG_0380 Mb0459c NC_000962.3 Mycobrowser_v2 CDS 542142 542852 . + 0 Rv0452 Rv0452 Possibly involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0452, (MTV037.16), len: 236 aa. Possible transcriptional regulator, similar to several putative TetR-family transcriptional regulators from Streptomyces coelicolor. Also similar in N-terminus to U1740Y|U15183|MLU15183_33 from Mycobacterium leprae (67 aa), FASTA score: (76.1% identity in 67 aa overlap). Contains probable helix-turn-helix motif at aa 44-65 (Score 1727, +5.07 SD). O53737 No regulatory proteins ML2376 MMAR_0773 Mb0460 NC_000962.3 Mycobrowser_v2 CDS 547586 549607 . - 0 Rv0457c Rv0457c Function unknown; hydrolyzes peptides and/or proteins. Probable peptidase Rv0457c, (MTCI429A.01, MTV038.01c), len: 673 aa. Probable peptidase, similar to many e.g. NP_102851.1|14022026|BAB48637.1 probable endopeptidase from Mesorhizobium loti (687 aa); Y4NA_RHISN|P55577 probable peptidase (726 aa), FASTA scores: opt: 1126, E(): 0, (40.9% identity in 491 aa overlap). Also similar to Mycobacterium tuberculosis protein MTCY369.26 FASTA score: (33.8% identity in 299 aa overlap). O07178 No intermediary metabolism and respiration ML2382 MMAR_0779 MSMEG_0899 Mb0466c NC_000962.3 Mycobrowser_v2 RBS 549661 549665 . + 0 Rv0458 Rv0458 Interconversion aldehyde and acid [catalytic activity: an aldehyde + NAD+ + H2O = an acid + NADH]. Probable aldehyde dehydrogenase Rv0458, (MTV038.02), len: 507 aa. Probable aldehyde dehydrogenase, highly similar to many, closest to P46369|THCA_RHOER EPTC-inducible aldehyde dehydrogenase from Rhodococcus erythropolis (506 aa), FASTA scores: opt: 2767, E(): 0, (79.7% identity in 507 aa overlap); AAC13641.1|AF029733 chloroacetaldehyde dehydrogenase from Xanthobacter autotrophicus (505 aa), FASTA scores: opt: 2563, E(): 0, (75.4% identity in 492 aa overlap); Q9RJZ6|DHAL_STRCO probable aldehyde dehydrogenase from Streptomyces coelicolor (507 aa). Also similar to other semialdehyde dehydrogenases in Mycobacterium tuberculosis e.g. Rv0768, Rv2858c. Belongs to the aldehyde dehydrogenases family. P63937 No intermediary metabolism and respiration ML2383 MMAR_0780 Mb0467 NC_000962.3 Mycobrowser_v2 CDS 549675 551198 . + 0 Rv0458 Rv0458 Interconversion aldehyde and acid [catalytic activity: an aldehyde + NAD+ + H2O = an acid + NADH]. Probable aldehyde dehydrogenase Rv0458, (MTV038.02), len: 507 aa. Probable aldehyde dehydrogenase, highly similar to many, closest to P46369|THCA_RHOER EPTC-inducible aldehyde dehydrogenase from Rhodococcus erythropolis (506 aa), FASTA scores: opt: 2767, E(): 0, (79.7% identity in 507 aa overlap); AAC13641.1|AF029733 chloroacetaldehyde dehydrogenase from Xanthobacter autotrophicus (505 aa), FASTA scores: opt: 2563, E(): 0, (75.4% identity in 492 aa overlap); Q9RJZ6|DHAL_STRCO probable aldehyde dehydrogenase from Streptomyces coelicolor (507 aa). Also similar to other semialdehyde dehydrogenases in Mycobacterium tuberculosis e.g. Rv0768, Rv2858c. Belongs to the aldehyde dehydrogenases family. P63937 No intermediary metabolism and respiration ML2383 MMAR_0780 Mb0467 NC_000962.3 Mycobrowser_v2 RBS 561722 561725 . - 0 Rv0458 Rv0458 Interconversion aldehyde and acid [catalytic activity: an aldehyde + NAD+ + H2O = an acid + NADH]. Probable aldehyde dehydrogenase Rv0458, (MTV038.02), len: 507 aa. Probable aldehyde dehydrogenase, highly similar to many, closest to P46369|THCA_RHOER EPTC-inducible aldehyde dehydrogenase from Rhodococcus erythropolis (506 aa), FASTA scores: opt: 2767, E(): 0, (79.7% identity in 507 aa overlap); AAC13641.1|AF029733 chloroacetaldehyde dehydrogenase from Xanthobacter autotrophicus (505 aa), FASTA scores: opt: 2563, E(): 0, (75.4% identity in 492 aa overlap); Q9RJZ6|DHAL_STRCO probable aldehyde dehydrogenase from Streptomyces coelicolor (507 aa). Also similar to other semialdehyde dehydrogenases in Mycobacterium tuberculosis e.g. Rv0768, Rv2858c. Belongs to the aldehyde dehydrogenases family. P63937 No intermediary metabolism and respiration ML2383 MMAR_0780 Mb0467 NC_000962.3 Mycobrowser_v2 CDS 551198 551689 . + 0 Rv0459 Rv0459 Function unknown Conserved hypothetical protein Rv0459, (MTV038.03), len: 163 aa. Conserved hypothetical protein, highly similar to other hypothetical proteins. Note that highly similar to products of unidentified ORFs in Xanthobacter autotrophicus, AF029733_2 (139 aa), and Rhodococcus erythropolis, REREUTP BC_1 (186 aa). Like MTV038.03, these ORF's are linked to aldehyde dehydrogenase genes. FASTA scores: AF0297|AF029733_2 (139 aa), opt: 439, E(): 6.2e-24, (50.0% identity in 126 aa overlap); and L24492|REREUTPBC_1 (186 aa), opt: 347, E(): 2.1e-17, (52.7% identity in 169 aa overlap). N-terminus also highly similar to AAA63041.1|U15183 ethanolamine permease (eutP) match from Mycobacterium leprae (53 aa). O53744 No conserved hypotheticals ML2384 MMAR_0781 MSMEG_0901 Mb0468 NC_000962.3 Mycobrowser_v2 CDS 551749 551988 . + 0 Rv0460 Rv0460 Function unknown Conserved hydrophobic protein Rv0460, (MTV038.04), len: 79 aa. Conserved hydrophobic protein, highly similar AAA63024.1|U15183 hypothetical protein from Mycobacterium leprae (56 aa), FASTA scores: opt: 197, E(): 3.7e-09, (63.8% identity in 47 aa overlap). O53745 No conserved hypotheticals ML2385 Mb0469 NC_000962.3 Mycobrowser_v2 CDS 552026 552550 . + 0 Rv0461 Rv0461 Unknown Probable transmembrane protein Rv0461, (MTV038.05), len: 174 aa (start uncertain). Probable transmembrane protein. Nucleotide position 552085 in the genome sequence has been corrected, A:G resulting in Q20Q. O53746 No cell wall and cell processes ML2386 Mb0470 NC_000962.3 Mycobrowser_v2 CDS 596759 597202 . + 0 Rv0506 mmpS2 Unknown Probable conserved membrane protein MmpS2 Rv0506, (MTCY20G9.33), len: 147 aa. Probable mmpS2, conserved membrane protein (see citation below), highly similar to other Mycobacterial proteins e.g. C-terminus of AAD44232.1|AF143772_38|AF143772|TmtpA from Mycobacterium avium (221 aa); P54880|MMS4_MYCLE|MMPS4 putative membrane protein from Mycobacterium leprae (154 aa), FASTA scores: opt: 392, E(): 1.3e-20, (43.7% identity in 151 aa overlap); and the putative membrane proteins from Mycobacterium tuberculosis MTV040_5, MTCY4D9_16, MTV037_15. Belongs to the MmpS family. Predicted to be an outer membrane protein (See Song et al., 2008). P65376 No cell wall and cell processes MMAR_0839 MSMEG_0186 Mb0518 NC_000962.3 Mycobrowser_v2 CDS 552614 554008 . + 0 Rv0462 lpdC Involved in energy metabolism. Lipoamide dehydrogenase is the E3 component of pyruvate dehydrogenase and the alpha-ketoacid dehydrogenase complex [catalytic activity: dihydrolipoamide + NAD(+) = lipoamide + NADH]. Also involved in antioxidant defense; LPDC|Rv0462, DLAT|Rv2215, AHPD|Rv2429, and AHPC|Rv2428 constitute an NADH-dependent peroxidase and peroxynitrite reductase that provides protection against oxidative stress. Dihydrolipoamide dehydrogenase LpdC (lipoamide reductase (NADH)) (lipoyl dehydrogenase) (dihydrolipoyl dehydrogenase) (diaphorase) Rv0462, (MTV038.06), len: 464 aa. LpdC (alternate gene name: TB49.2, CIP50), dihydrolipoamide dehydrogenase (see Argyrou & Blanchard 2001), equivalent to AAA63016.1|U15183 lipoamide dehydrogenase from Mycobacterium leprae (467 aa), FASTA scores: opt: 2583, E(): 0, (83.1% identity in 467 aa overlap). Also similar to to many e.g. P50970|DLDH_ZYMMO|X82291|ZMLPD_1 dihydrolipoamide dehydrogenase from Z.mobilis (466 aa), FASTA scores: opt: 1198, E(): 0, (42.4 % identity in 465 aa overlap); etc. Belongs to the pyridine nucleotide-disulfide oxidoreductases class-I. Binds to coronin-1 in BCG and M. tuberculosis - coronin-1 is retained on phagosomes and phagosome maturation is arrested (See Deghmane et al., 2007). LpdC|Rv0462 co-immunoprecipitates with DlaT|Rv2215 (in lpdC|Rv0462 mutant) and with BkdC|Rv2495c (in dlaT|Rv2215 mutant) (See Venugopal et al., 2011). P66004 No intermediary metabolism and respiration ML2387 MMAR_0785 MSMEG_0903 Mb0471 NC_000962.3 Mycobrowser_v2 CDS 554016 554309 . + 0 Rv0463 Rv0463 Unknown Probable conserved membrane protein Rv0463, (MTV038.07), len: 97 aa. Probable conserved transmembrane protein, highly similar to AAA63017.1|U15183 hypothetical protein from Mycobacterium leprae (101 aa), FASTA scores: opt: 364, E(): 4e-21, (57.9% identity in 95 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53748 No cell wall and cell processes ML2388 MMAR_0786 MSMEG_0904 Mb0472 NC_000962.3 Mycobrowser_v2 CDS 1241115 1241390 . - 0 Rv1116A Rv1116A Function unknown Conserved hypothetical protein (fragment) Rv1116A, len: 91 aa. Conserved hypothetical protein (possibly gene fragment), similar to C-terminal part of Rv1646|Z85982_9 from Mycobacterium tuberculosis (310 aa), FASTA scores: opt: 301, E(): 9.3e-13, (68.05% identity in 72 aa overlap). Also overlaps gene on other strand, Rv1116, at 3'-end. Q8VK68 No conserved hypotheticals Mb1147c NC_000962.3 Mycobrowser_v2 CDS 554882 556306 . - 0 Rv0465c Rv0465c Possibly involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv0465c, (MTV038.09c), len: 474 aa. Probable transcriptional regulator, highly similar to AC44331.1|AL596102 putative DNA-binding protein from Streptomyces coelicolor (489 aa); and similar to several hypothetical proteins and others transcriptional regulators. Some similarity in N-terminal region (1-100 aa) with repressors e.g. P06153|RPC_BPPH1 immunity repressor protein (144 aa), FASTA scores: opt: 130, E(): 0.084,(27.0% identity in 100 aa overlap). Very similar to Rv1129c|Z95585|MTCY22G8.18c from Mycobacterium tuberculosis (486 aa), FASTA scores: opt: 1475, E(): 0, (47.4% identity in 468 aa overlap). Contains probable helix-turn-helix motif at aa 19-40 (1827, +5.41 SD). O53750 No regulatory proteins ML2464 MMAR_0790 MSMEG_0906 Mb0474c NC_000962.3 Mycobrowser_v2 CDS 556458 557252 . + 0 Rv0466 Rv0466 Function unknown Conserved protein Rv0466, (MTV038.10), len: 264 aa. Conserved protein, equivalent to CAC31980.1|AL583925 conserved hypothetical protein from Mycobacterium leprae (264 aa). Similar to Rv2001|Z74025|MTCY39.17c hypothetical 28.7 KDA protein from Mycobacterium tuberculosis (250 aa), FASTA scores: opt: 592, E(): 0, (38.0% identity in 263 aa overlap). Some similarity to several thioesterases e.g. Q42561|ATACPTE17_1 acyl-(acyl carrier protein) thioester from A. thaliana (362 aa), FASTA scores: E(): 0.0092, (24.4% identity in 197 aa overlap). O53751 No conserved hypotheticals ML2463 MMAR_0791 MSMEG_0909 Mb0475 NC_000962.3 Mycobrowser_v2 RBS 557514 557520 . + 0 Rv0467 icl1 Involved in glyoxylate bypass (at the first step), an alternative to the tricarboxylic acid cycle (in bacteria, plants, and fungi) [catalytic activity: isocitrate = succinate + glyoxylate]. Involved in the persistence in the host. Isocitrate lyase Icl (isocitrase) (isocitratase) Rv0467, (MTV038.11), len: 428 aa. Icl1, isocitrate lyase (see citations below), highly similar to many, closest to Z29367|RFISCILY_1 from R. fascians (429 aa), FASTA scores: opt: 2359, E(): 0, (80.7% identity in 429 aa overlap). Belongs to the isocitrate lyase family. Has 2-methyl-isocitrate lyase (MCL) activity in M. tuberculosis Erdman (See Munoz-Elias et al., 2006; Gould et al., 2006). Predicted possible vaccine candidate (See Zvi et al., 2008). P0A5H3 No intermediary metabolism and respiration ML2462 MMAR_0792 MSMEG_0911 Mb0476 NC_000962.3 Mycobrowser_v2 CDS 557527 558813 . + 0 Rv0467 icl1 Involved in glyoxylate bypass (at the first step), an alternative to the tricarboxylic acid cycle (in bacteria, plants, and fungi) [catalytic activity: isocitrate = succinate + glyoxylate]. Involved in the persistence in the host. Isocitrate lyase Icl (isocitrase) (isocitratase) Rv0467, (MTV038.11), len: 428 aa. Icl1, isocitrate lyase (see citations below), highly similar to many, closest to Z29367|RFISCILY_1 from R. fascians (429 aa), FASTA scores: opt: 2359, E(): 0, (80.7% identity in 429 aa overlap). Belongs to the isocitrate lyase family. Has 2-methyl-isocitrate lyase (MCL) activity in M. tuberculosis Erdman (See Munoz-Elias et al., 2006; Gould et al., 2006). Predicted possible vaccine candidate (See Zvi et al., 2008). P0A5H3 No intermediary metabolism and respiration ML2462 MMAR_0792 MSMEG_0911 Mb0476 NC_000962.3 Mycobrowser_v2 RBS 558885 558891 . + 0 Rv0468 fadB2 Butyrate/butanol-producing pathway [catalytic activity: (S)-3-hydroxybutanoyl-CoA + NADP+ = 3-acetoacetyl-CoA + NADPH] 3-hydroxybutyryl-CoA dehydrogenase FadB2 (beta-hydroxybutyryl-CoA dehydrogenase) (BHBD) Rv0468, (MTV038.12), len: 286 aa. fadB2, 3-hydroxybutyryl-CoA dehydrogenase, equivalent to CAC31978.1|AL583925 3-hydroxyacyl-CoA dehydrogenase from Mycobacterium leprae (287 aa). Also similar to many 3-hydroxybutyryl-CoA dehydrogenases e.g. U32229|BJU32229_1 beta-hydroxybutyryl coenzyme A dehydrogenase from Bradyrhizobium japonicum (293 aa), FASTA scores: opt: 771, E(): 0, (45.7% identity in 282 aa overlap). Belongs to the 3-hydroxyacyl-CoA dehydrogenase family. O53753 No lipid metabolism ML2461 MMAR_0793 MSMEG_0912 Mb0477 NC_000962.3 Mycobrowser_v2 CDS 558895 559755 . + 0 Rv0468 fadB2 Butyrate/butanol-producing pathway [catalytic activity: (S)-3-hydroxybutanoyl-CoA + NADP+ = 3-acetoacetyl-CoA + NADPH] 3-hydroxybutyryl-CoA dehydrogenase FadB2 (beta-hydroxybutyryl-CoA dehydrogenase) (BHBD) Rv0468, (MTV038.12), len: 286 aa. fadB2, 3-hydroxybutyryl-CoA dehydrogenase, equivalent to CAC31978.1|AL583925 3-hydroxyacyl-CoA dehydrogenase from Mycobacterium leprae (287 aa). Also similar to many 3-hydroxybutyryl-CoA dehydrogenases e.g. U32229|BJU32229_1 beta-hydroxybutyryl coenzyme A dehydrogenase from Bradyrhizobium japonicum (293 aa), FASTA scores: opt: 771, E(): 0, (45.7% identity in 282 aa overlap). Belongs to the 3-hydroxyacyl-CoA dehydrogenase family. O53753 No lipid metabolism ML2461 MMAR_0793 MSMEG_0912 Mb0477 NC_000962.3 Mycobrowser_v2 CDS 1108172 1108504 . - 0 Rv0991c Rv0991c Function unknown Conserved serine rich protein Rv0991c, (MTCI237.05c), len: 110 aa. Conserved ser-rich protein (especially in C-terminus), highly similar to N-terminus of NP_301255.1|NC_002677 conserved hypothetical protein (Ser-rich C-terminus) from Mycobacterium leprae (99 aa). Also highly similar to SCE22.04|AB90971.1|AL355832 hypothetical protein from Streptomyces coelicolor (110 aa); and similar to others. O05574 No conserved hypotheticals ML0180 MMAR_4523 MSMEG_5479 Mb1018c NC_000962.3 Mycobrowser_v2 RBS 1108510 1108514 . - 0 Rv0991c Rv0991c Function unknown Conserved serine rich protein Rv0991c, (MTCI237.05c), len: 110 aa. Conserved ser-rich protein (especially in C-terminus), highly similar to N-terminus of NP_301255.1|NC_002677 conserved hypothetical protein (Ser-rich C-terminus) from Mycobacterium leprae (99 aa). Also highly similar to SCE22.04|AB90971.1|AL355832 hypothetical protein from Streptomyces coelicolor (110 aa); and similar to others. O05574 No conserved hypotheticals ML0180 MMAR_4523 MSMEG_5479 Mb1018c NC_000962.3 Mycobrowser_v2 RBS 559874 559877 . + 0 Rv0469 umaA Involved in mycolic acid modification or synthesis. Possible mycolic acid synthase UmaA Rv0469, (MTV038.13), len: 286 aa. Possible umaA, mycolic acid synthase (see citations below), highly similar to CAC30854.1|AL583923 methyl mycolic acid synthase 1 from Mycobacterium leprae (286 aa); and CAC31976.1|AL583925 Mycolic acid synthase from Mycobacterium leprae (295 aa), FASTA scores: opt: 1402, E(): 0, (69.6% identity in 286 aa overlap). Also very similar to mycobacterial methyltransferases e.g. U77466|CmaD|MBU77466_1 (286 aa); MTCY20H10.26c|Z92772|MTY20H10_27 (296 aa); highly similar to CFA1_MYCTU|Q11195|U66108|MTU66108_1 cyclopropane-fatty-acyl-phospholipid synthase 1 (287 aa), FASTA scores: opt: 1360, E(): 0, (67.8% identity in 286 aa overlap) (see citation below); and very similar also to methoxy mycolic acid synthase 1 from Mycobacterium tuberculosis e.g. MTU66108_1 (286 aa). Note that previously known as umaA1. Q6MX39 No lipid metabolism ML2460 MMAR_0794 MSMEG_0913 Mb0478 NC_000962.3 Mycobrowser_v2 CDS 559888 560748 . + 0 Rv0469 umaA Involved in mycolic acid modification or synthesis. Possible mycolic acid synthase UmaA Rv0469, (MTV038.13), len: 286 aa. Possible umaA, mycolic acid synthase (see citations below), highly similar to CAC30854.1|AL583923 methyl mycolic acid synthase 1 from Mycobacterium leprae (286 aa); and CAC31976.1|AL583925 Mycolic acid synthase from Mycobacterium leprae (295 aa), FASTA scores: opt: 1402, E(): 0, (69.6% identity in 286 aa overlap). Also very similar to mycobacterial methyltransferases e.g. U77466|CmaD|MBU77466_1 (286 aa); MTCY20H10.26c|Z92772|MTY20H10_27 (296 aa); highly similar to CFA1_MYCTU|Q11195|U66108|MTU66108_1 cyclopropane-fatty-acyl-phospholipid synthase 1 (287 aa), FASTA scores: opt: 1360, E(): 0, (67.8% identity in 286 aa overlap) (see citation below); and very similar also to methoxy mycolic acid synthase 1 from Mycobacterium tuberculosis e.g. MTU66108_1 (286 aa). Note that previously known as umaA1. Q6MX39 No lipid metabolism ML2460 MMAR_0794 MSMEG_0913 Mb0478 NC_000962.3 Mycobrowser_v2 CDS 566508 566771 . + 0 Rv0476 Rv0476 Unknown Possible conserved transmembrane protein Rv0476, (MTCY20G9.02), len: 87 aa. Possible conserved transmembrane protein, equivalent to CAC31970.1|AL583925 conserved membrane protein from Mycobacterium leprae (95 aa). Also highly similar to CAC04036.1|AL391406 putative membrane protein from Streptomyces coelicolor (113 aa). Contains PS00606 Beta-ketoacyl synthases active site. P64695 No cell wall and cell processes ML2453 Mb0486 NC_000962.3 Mycobrowser_v2 CDS 600098 600391 . + 0 Rv0508 Rv0508 Function unknown Conserved hypothetical protein Rv0508, (MTCY20G9.35), len: 97 aa. Conserved hypothetical protein, showing similarity with T36269|5123666|CAB45355.1|AL079345 probable redoxin from Streptomyces coelicolor (101 aa), FASTA scores: opt: 160, E(): 3.4e-05, (33.3% identity in 75 aa overlap); and E81943|NMA0966 probable thioredoxin from Neisseria meningitidis group a strain Z2491 (77 aa). P64727 No conserved hypotheticals ML2423 MMAR_0841 MSMEG_0951 Mb0520 NC_000962.3 Mycobrowser_v2 RBS 565010 565014 . + 0 Rv0474 Rv0474 Possibly involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv0474, (MTV038.18), len: 140 aa. Probable transcriptional regulator, highly similar to others e.g. CAC04034.1|AL391406 putative DNA-binding protein from Streptomyces coelicolor (141 aa); N-terminus of NP_104173.1|14023352|BAB49959.1|AP003000 transcriptional regulator from Mesorhizobium loti (219 aa); N-terminus of A83618|PA0225 probable transcription regulator from Pseudomonas aeruginosa (179 aa); SINR_BACSU|P06533 sinr protein from Bacillus subtilis (111 aa), FASTA scores: opt: 147, E(): 8.9e-06, (30.6% identity in 111 aa overlap). Also similar to other hypothetical proteins e.g. X66407|RRPHAS_1|ORF1 from Rhodococcus ruber (171 aa), FASTA scores: opt: 280, E(): 4.8e-12, (43.6% identity in 117 aa overlap). Also similar to Rv2745c from Mycobacterium tuberculosis. Contains probable helix-turn-helix domain at aa 35-56 (Score 1709, +5.01 SD). O53759 No regulatory proteins ML2455 MMAR_0799 MSMEG_0918 Mb0484 NC_000962.3 Mycobrowser_v2 CDS 565021 565443 . + 0 Rv0474 Rv0474 Possibly involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv0474, (MTV038.18), len: 140 aa. Probable transcriptional regulator, highly similar to others e.g. CAC04034.1|AL391406 putative DNA-binding protein from Streptomyces coelicolor (141 aa); N-terminus of NP_104173.1|14023352|BAB49959.1|AP003000 transcriptional regulator from Mesorhizobium loti (219 aa); N-terminus of A83618|PA0225 probable transcription regulator from Pseudomonas aeruginosa (179 aa); SINR_BACSU|P06533 sinr protein from Bacillus subtilis (111 aa), FASTA scores: opt: 147, E(): 8.9e-06, (30.6% identity in 111 aa overlap). Also similar to other hypothetical proteins e.g. X66407|RRPHAS_1|ORF1 from Rhodococcus ruber (171 aa), FASTA scores: opt: 280, E(): 4.8e-12, (43.6% identity in 117 aa overlap). Also similar to Rv2745c from Mycobacterium tuberculosis. Contains probable helix-turn-helix domain at aa 35-56 (Score 1709, +5.01 SD). O53759 No regulatory proteins ML2455 MMAR_0799 MSMEG_0918 Mb0484 NC_000962.3 Mycobrowser_v2 CDS 560848 561711 . - 0 Rv0470c pcaA Involved in the mycolic acid modification or synthesis; essential for the cyclopropanation function. Required for cording and mycolic acid cyclopropane ring synthesis in the cell wall. Mycolic acid synthase PcaA (cyclopropane synthase) Rv0470c, (MTV038.14), len: 287 aa. PcaA (previously known as umaA2), mycolic acid synthase (cyclopropane synthase) (see citations below), equivalent to CAC31976.1|AL583925 Mycolic acid synthase from Mycobacterium leprae (295 aa); and highly similar to S72886|B2168_F3_130|467038|AAA17222.1|U00018 hypothetical protein from Mycobacterium leprae (308 aa); Q11195|CFA1_MYCTU cyclopropane-fatty-acyl-phospholipid synthase 1 (cyclopropane mycolic acid synthase 1) (287 aa) (see Glickman et al., 2000); U27357|MTU27357_1 cyclopropane mycolic acid synthase from Mycobacterium tuberculosis (287 aa), FASTA scores: opt: 1415, E(): 0, (72.8% identity in 287 aa overlap); and related enzymes e.g. MTCY20H10.25c|Z92772|MTY20H10_26 (287 aa), FASTA scores: opt: 1387, E(): 0, (72.5% identity in 287 aa overlap). Q7D9R5 No lipid metabolism ML2459 MMAR_0796 Mb0479c NC_000962.3 Mycobrowser_v2 CDS 561854 562294 . - 0 Rv0470A Rv0470A Unknown Hypothetical protein Rv0470A, len: 146 aa. Hypothetical unknown protein. GC plot suggests CDS for Cys-rich protein, could possibly be continuation of Rv0471c but no frameshift found to allow this. Sequence same in Mycobacterium bovis and Mycobacterium tuberculosis strain CDC1551. Weak hits to Cys-rich region (aa 258-314) of D63395|D63395_1 mRNA for NOTCH4 from Homo sapiens (1095 aa), FASTA scores: opt: 132, E(): 1.1, (39.35% identity in 61 aa overlap). Q8VKK9 No conserved hypotheticals Mb0480c NC_000962.3 Mycobrowser_v2 CDS 562225 562713 . - 0 Rv0471c Rv0471c Unknown Hypothetical protein Rv0471c, (MTV038.15c), len: 162 aa. Hypothetical unknown protein. O53756 No conserved hypotheticals ML2458 Mb0481c NC_000962.3 Mycobrowser_v2 CDS 562723 563427 . - 0 Rv0472c Rv0472c Possibly involved in transcriptional mechanism. Probable transcriptional regulatory protein (possibly TetR-family) Rv0472c, (MTV038.16c), len: 234 aa. Probable regulatory protein, possibly TetR family, equivalent to CAC31974.1|AL583925 possible TetR-family transcriptional regulator from Mycobacterium leprae (233 aa). Also similar to CAC01492.1|AL391017 putative transcriptional regulatory protein from Streptomyces coelicolor (218 aa); and CAC01371.1|AL390975 putative TetR-family transcriptional regulator from Streptomyces coelicolor (228 aa). Also similar to AL0212|MTV012_65 from Mycobacterium tuberculosis (246 aa), FASTA scores: opt: 327, E(): 1.8e-15, (31.0% identity in 232 aa overlap); and Z95120|MTCY07D11.18c (228 aa), FASTA scores: opt: 190, E(): 4.4e-06, (23.1% identity in 186 aa overlap). Contains probable helix-turn-helix doimain at aa 45-66 (Score 1429, +4.05 SD). O53757 No regulatory proteins ML2457 MMAR_0797 MSMEG_0916 Mb0482c NC_000962.3 Mycobrowser_v2 CDS 563564 564934 . + 0 Rv0473 Rv0473 Unknown Possible conserved transmembrane protein Rv0473, (MTV038.17), len: 456 aa. Possible conserved transmembrane protein, showing some similarity to hypothetical proteins e.g. NP_102800.1|14021975|BAB48586.1|AP002996 hypothetical protein from Mesorhizobium loti (431 aa); P39385|YJIN_ECOLI|YJIN|B4336 hypothetical 48.2 kDa protein (potential integral membrane protein) from Escherichia coli strain K12 (426 aa), FASTA scores: opt: 396, E(): 9.8e-19, (31.8 % identity in 424 aa overlap); etc. Nucleotide position 563577 in the genome sequence has been corrected, A:G resulting in K5R. O53758 No cell wall and cell processes ML2456 MMAR_0798 MSMEG_0917 Mb0483 NC_000962.3 Mycobrowser_v2 RBS 715352 715356 . + 0 Rv0622 Rv0622 Unknown Possible membrane protein Rv0622, (MTCY20H10.03), len: 315 aa. Possible membrane protein; contains potential membrane spanning region. Shows weak similarity with Mycobacterium tuberculosis hypothetical proteins Rv1804c, Rv1810, etc. Start changed since first submission (-26 aa). P96912 No cell wall and cell processes MSMEG_6737 Mb0638 NC_000962.3 Mycobrowser_v2 CDS 715370 716317 . + 0 Rv0622 Rv0622 Unknown Possible membrane protein Rv0622, (MTCY20H10.03), len: 315 aa. Possible membrane protein; contains potential membrane spanning region. Shows weak similarity with Mycobacterium tuberculosis hypothetical proteins Rv1804c, Rv1810, etc. Start changed since first submission (-26 aa). P96912 No cell wall and cell processes MSMEG_6737 Mb0638 NC_000962.3 Mycobrowser_v2 RBS 565784 565790 . + 0 Rv0475 hbhA Required for extrapulmonary dissemination. Mediates adherence to epithelial cells by binding to sulfated glycoconjugates present at the surface of these cells; binds heparin, dextran sulfate, fucoidan and chondroitin sulfate. Promotes hemagglutination of erythrocytes of certain host species. Induces mycobacterial aggregation. Iron-regulated heparin binding hemagglutinin HbhA (adhesin) Rv0475, hbhA (MTCY20G9.01), len: 199 aa. HbhA, iron-regulated heparin-binding hemagglutinin (see citations below), equivalent to CAC31971.1|AL583925 possible hemagglutinin from Mycobacterium leprae (188 aa). Contains possible N-terminal signal sequence and K-a-rich region at C-terminus: subcellular location: surface associated. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P0A5P6 No cell wall and cell processes ML2454 MMAR_0800 MSMEG_0919 Mb0485 NC_000962.3 Mycobrowser_v2 CDS 565797 566396 . + 0 Rv0475 hbhA Required for extrapulmonary dissemination. Mediates adherence to epithelial cells by binding to sulfated glycoconjugates present at the surface of these cells; binds heparin, dextran sulfate, fucoidan and chondroitin sulfate. Promotes hemagglutination of erythrocytes of certain host species. Induces mycobacterial aggregation. Iron-regulated heparin binding hemagglutinin HbhA (adhesin) Rv0475, hbhA (MTCY20G9.01), len: 199 aa. HbhA, iron-regulated heparin-binding hemagglutinin (see citations below), equivalent to CAC31971.1|AL583925 possible hemagglutinin from Mycobacterium leprae (188 aa). Contains possible N-terminal signal sequence and K-a-rich region at C-terminus: subcellular location: surface associated. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P0A5P6 No cell wall and cell processes ML2454 MMAR_0800 MSMEG_0919 Mb0485 NC_000962.3 Mycobrowser_v2 CDS 567222 567896 . + 0 Rv0478 deoC Involved in nucleotide and deoxyribonucleotide catabolism [catalytic activity: 2-deoxy-D-ribose 5-phosphate = D-glyceraldehyde 3-phosphate + acetaldehyde]. Probable deoxyribose-phosphate aldolase DeoC (phosphodeoxyriboaldolase) (deoxyriboaldolase) Rv0478, (MTCY20G9.04), len: 224 aa. Probable deoC, deoxyribose-phosphate aldolase, equivalent to Q9CB45|DEOC_MYCLE deoxyribose-phosphate aldolase from Mycobacterium leprae (226 aa). Also highly similar to others e.g. DEOC_BACSU|P39121 from Bacillus subtilis (214 aa), FASTA scores: opt: 543, E(): 1.4e-26, (45.9% identity in 209 aa overlap); etc. Belongs to the DEOC/FBAB family of aldolases, DEOC subfamily. P63929 No intermediary metabolism and respiration ML2451 MMAR_0803 MSMEG_0922,MSMEG_3089 Mb0488 NC_000962.3 Mycobrowser_v2 CDS 567921 568967 . - 0 Rv0479c Rv0479c Unknown Probable conserved membrane protein Rv0479c, (MTCY20G9.04c), len: 348 aa. Probable conserved membrane protein, equivalent to CAC31967.1|AL583925 possible secreted protein from Mycobacterium leprae (254 aa); and C-terminus highly similar to AAF74996.1|AF143402_1|AF143402 putative multicopper oxidase from Mycobacterium avium (149 aa). Contains hydrophobic domain in centre of protein. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P64699 No cell wall and cell processes ML2450 MMAR_0804 MSMEG_0923 Mb0489c NC_000962.3 Mycobrowser_v2 CDS 568964 569806 . - 0 Rv0480c Rv0480c Function unknown; hydrolytic enzyme probably involved in cellular metabolism. Possible amidohydrolase Rv0480c, (MTCY20G9.06c), len: 280 aa. Possible amidohydrolase, highly similar to NP_302587.1|NC_002677|CAC31966.1|AL583925 putative hydrolase from Mycobacterium leprae (271 aa). Also similar to other hydrolases and hypothetical proteins e.g. NP_601985.1|NC_003450 Predicted amidohydrolase from Corynebacterium glutamicum (266 aa); NP_459623.1|NC_003197 putative hydrolase from Salmonella typhimurium LT2 (262 aa); AL096822|SCGD3_8|NP_627996.1|NC_003888 probable hydrolase from Streptomyces coelicolor (264 aa), FASTA scores: opt: 368, E(): 6.1e-15, (34.2% identity in 272 aa overlap); YAUB_SCHPO|Q10166 hypothetical 35.7 kDa protein c26a3.11 from S. pombe (322 aa), FASTA scores: opt: 338, E():1.4e-13, (30.3% identity in 277 aa overlap); etc. Start changed since first submission (-60 aa). Q11146 No intermediary metabolism and respiration ML2449 MMAR_0805 MSMEG_0924 Mb0490c NC_000962.3 Mycobrowser_v2 CDS 569988 570512 . - 0 Rv0481c Rv0481c Unknown Hypothetical protein Rv0481c, (MTCY20G9.07c), len: 174 aa. Hypothetical unknown protein. P64701 No conserved hypotheticals ML2448 MMAR_0807 MSMEG_0927 Mb0491c NC_000962.3 Mycobrowser_v2 CDS 586394 587380 . + 0 Rv0496 Rv0496 Function unknown Conserved hypothetical protein Rv0496, (MTCY20G9.22), len: 328 aa. Conserved hypothetical protein, highly similar to S72894|467046|AAA17230.1|U00018 exopolyphosphatase ppx from Mycobacterium leprae (406 aa), FASTA scores: opt: 1902, E(): 0, (86.6% identity in 343 aa overlap); and P54882|Y496_MYCLE|ML2434|13094008|CAC31951.1|AL583925 hypothetical 36.2 KDA protein from Mycobacterium leprae (339 aa). Also highly similar to hypothetical proteins and exopolyphosphatases e.g. Q9X8H1|Y715_STRCO|SCE7.15c hypothetical protein from Streptomyces coelicolor (309 aa). C-terminal region similar to CGU31224_1|Q46054 protein similar to ppx gene product of Mycobacterium leprae from Cornybacterium glutamicum (140 aa), FASTA scores: opt: 615, E(): 2.7e-33, (70.9% identity in 134 aa overlap). P65786 No conserved hypotheticals ML2434 MMAR_0822 MSMEG_0939 Mb0507 NC_000962.3 Mycobrowser_v2 CDS 570539 571648 . + 0 Rv0482 murB Involved in cell wall formation; peptidoglycan biosynthesis [catalytic activity: UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH]. Probable UDP-N-acetylenolpyruvoylglucosamine reductase MurB (UDP-N-acetylmuramate dehydrogenase) Rv0482, (MTCY20G9.08), len: 369 aa. Probable murB, UDP-N-acetylenolpyruvoylglucosamine reductase (see citation below), equivalent to CAC31964.1|AL583925 UDP-N-acetylenolpyruvoylglucosamine reductase from Mycobacterium leprae (367 aa). Also highly similar to others e.g. MURB_ECOLI|P08373 UDP-N-acetylenolpyruvoylglucosamine reductase from Escherichia coli (342 aa), FASTA scores: opt: 292, E(): 6.3e-12, (33.5% identity in 355 aa overlap); etc. Belongs to the MurB family. Cofactor: FAD. P65460 No cell wall and cell processes ML2447 MMAR_0808 MSMEG_0928 Mb0492 NC_000962.3 Mycobrowser_v2 CDS 585424 586314 . - 0 Rv0495c Rv0495c Function unknown Conserved hypothetical protein Rv0495c, (MTCY20G9.21c), len: 296 aa. Conserved hypothetical protein, highly similar to S72915|B2168_F1_37 hypothetical protein from Mycobacterium leprae (323 aa), FASTA scores: opt: 1615, E(): 0, (82.7% identity in 271 aa overlap); and P54579|Y495_MYCLE|ML243|13094009|CAC31952.1|AL583925 conserved hypothetical protein from Mycobacterium leprae (277 aa). Also highly similar to Q9X8H2|Y716_STRCO|SCE7.16 hypothetical protein from Streptomyces coelicolor (271 aa). P64713 No conserved hypotheticals ML2435 MMAR_0821 MSMEG_0938 Mb0506c NC_000962.3 Mycobrowser_v2 CDS 571710 573065 . + 0 Rv0483 lprQ Unknown Probable conserved lipoprotein LprQ Rv0483, (MTCY20G9.09), len: 451 aa. Probable lprQ, conserved lipoprotein, equivalent to CAC31963.1|AL583925|ML2446 possible lipoprotein from Mycobacterium leprae (441 aa); appears longer than ML2446, so start may be further downstream. Shows also similarity with MLCL383_24|O07707 hypothetical 43.6 kDa protein from Mycobacterium leprae; and to Q49706|B1496_F2_81 (271 aa). Similar to others lipoproteins from other organisms. Also similar to several Mycobacterium tuberculosis hypothetical proteins e.g. Rv0116c, Rv0192, Rv1433, Rv2518c. Contains potential N-terminal signal sequence and appropriately positioned PS00013 prokaryotic membrane lipoprotein lipid attachment site. P64703 No cell wall and cell processes ML2446 MMAR_0809 MSMEG_0929 Mb0493 NC_000962.3 Mycobrowser_v2 CDS 573046 573801 . - 0 Rv0484c Rv0484c Function unknown; possibly involved in cellular metabolism. Probable short-chain type oxidoreductase Rv0484c, (MTCY20G9.10c), len: 251 aa. Probable short-chain oxidoreductase, highly similar to others e.g. T36118|4678912|CAB41284.1|AL049707 probable oxidoreductase from Streptomyces coelicolor (260 aa); YDFG_HAEIN|P45200|HI1430 hypothetical oxidoreductase (SDR family) from Haemophilus influenzae (252 aa), FASTA scores: opt: 496, E(): 7.9e-25, (35.0 % identity in 243 aa overlap); etc. Belongs to the short-chain dehydrogenases/reductases (SDR) family. Strong similarity, to bacterial YDFG homologs. Q11150 No intermediary metabolism and respiration ML2445 MMAR_0810 MSMEG_0930 Mb0494c NC_000962.3 Mycobrowser_v2 CDS 933003 934031 . - 0 Rv0837c Rv0837c Unknown Hypothetical protein Rv0837c, (MTV043.30c), len: 342 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53849 No conserved hypotheticals Mb0860c NC_000962.3 Mycobrowser_v2 CDS 573984 575300 . + 0 Rv0485 Rv0485 Possibly involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0485, (MTCY20G9.11), len: 438 aa. Possible transcriptional repressor, member of the NAGC/XYLR repressor family; similar to several e.g. D87820_3|O32446|D82254 NAGC N-acetylglucosamine repressor from Vibrio cholerae (404 aa), FASTA scores: opt: 378, E(): 1.2e-17, (26.9% identity in 350 aa overlap); NAGC_ECOLI|P15301 N-acetylglucosamine repressor from Escherichia coli (406 aa), FASTA scores: opt: 305, E(): 1.8e-12, (21.8% identity in 357 aa overlap); etc. P64705 No regulatory proteins ML2444 MMAR_0811 MSMEG_0932 Mb0495 NC_000962.3 Mycobrowser_v2 ncRNA 575033 575069 . + 0 MTB000060 mcr19 Unknown Fragment of putative small regulatory RNA mcr19, fragment of putative small regulatory RNA (See DiChiara et al., 2010), cloned from M. bovis BCG Pasteur; ends not mapped, 66-82 nt band detected by Northern blot in M. bovis BCG Pasteur. No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 632372 633034 . + 0 Rv0540 Rv0540 Function unknown Conserved hypothetical protein Rv0540, (MTCY25D10.19), len: 220 aa. Conserved hypothetical protein, similar to hypothetical proteins from Streptomyces coelicolor: CAB76990.1|AL159178 (213 aa); N-terminus of BAA84086.1|AB032065 (446 aa); and CAB61872.1|AL133252|SCE46_21 (210 aa), FASTA scores: opt: 267, E(): 5.3e-10, (32.7% identity in 202 aa overlap). Also some similarity with D90913_63|PCC6803 from Synecho cystis sp (211 aa), FASTA scores: opt: 189, E(): 4.7e-06, (25.3 identity in 194 aa overlap). O06406 No conserved hypotheticals MMAR_0886 MSMEG_0996 Mb0554 NC_000962.3 Mycobrowser_v2 CDS 575348 576790 . + 0 Rv0486 mshA Involved in the first step of mycothiol biosynthesis Glycosyltransferase MshA Rv0486, (MTCY20G9.12), len: 480 aa. MshA, glycosyltransferase (see citations below), highly similar to P54138|Y486_MYCLE|ML2443 possible glycosyl transferase from Mycobacterium leprae (428 aa); and S72892|B2168_C2_201 probable hexosyltransferase from Mycobacterium leprae (409 aa), FASTA scores: opt: 2375, E(): 0, (86.4% identity in 413 aa overlap). Also highly similar to CAC04040.1|AL391406 putative transferase from Streptomyces coelicolor (496 aa); and similar to various transferases e.g. NP_437172.1|NC_003078 putative membrane-anchored glycosyltransferase protein from Sinorhizobium meliloti (416 aa); O26550|U67601_1 LPS biosynthesis related protein from Methanococcus jannaschii (411 aa), FASTA score: (25.3% identity in 387 aa overlap); etc. Also similar to CAC87824.1|AJ316594 putative sucrose-phosphate synthase from Nostoc punctiforme (422 aa). Contains a match to Pfam entry PF00534 glycosyl_transf_1 - Glycosyl transferases group 1. P64707 No intermediary metabolism and respiration ML2443 MMAR_0812 MSMEG_0933 Mb0496 NC_000962.3 Mycobrowser_v2 CDS 576787 577338 . + 0 Rv0487 Rv0487 Function unknown Conserved hypothetical protein Rv0487, (MTCY20G9.13), len: 183 aa. Conserved hypothetical protein, highly similar to P54139|Y487_MYCLE|U00018_38|ML2442 hypothetical 20.8 KDA protein from Mycobacterium leprae (184 aa), FASTA scores: opt: 760, E(): 2.4 e-34, (73.0% identity in 159 aa overlap). Also highly similar to CAC04041.1|AL391406 conserved hypothetical protein from Streptomyces coelicolor (168 aa). P64709 No conserved hypotheticals ML2442 MMAR_0813 MSMEG_0934 Mb0497 NC_000962.3 Mycobrowser_v2 CDS 2544698 2545225 . + 0 Rv2270 lppN Unknown Probable lipoprotein LppN Rv2270, (MTCY339.40c), len: 175 aa. Probable lppN, lipoprotein; has appropriately positioned prokaryotic membrane lipoprotein attachment site PS00013. Q50693 No cell wall and cell processes MMAR_3366 Mb2293 NC_000962.3 Mycobrowser_v2 CDS 2261098 2261688 . + 0 Rv2014 Rv2014 Required for the transposition of an insertion element. Transposase Rv2014, (MTCY39.03c), len: 196 aa. Transposase, similar to insertion elements; possibly made by frameshifting with respect to Rv2013. Length changed since first submission. Q10843 No insertion seqs and phages Mb2037 NC_000962.3 Mycobrowser_v2 CDS 577664 578269 . + 0 Rv0488 Rv0488 Unknown; possibly involved in transport of lysine across the membrane. Probable conserved integral membrane protein Rv0488, (MTCY20G9.14), len: 201 aa. Probable conserved integral membrane protein, LysE family possibly involved in transport of Lysine, similar to others and conserved hypothetical proteins e.g. AB93746.1|AL357613 putative membrane transport protein from Streptomyces coelicolor (204 aa); D83100|PA4365 probable transporter from Pseudomonas aeruginosa (200 aa); YGGA_ECOLI|P11667 hypothetical 21.7 kDa protein from Escherichia coli (197 aa), FASTA scores: opt: 382, E(): 1.1e-19, (39.1% identity in 179 aa overlap); CGLYSEG_2 C|P94633 lysine exporter protein (236 aa), FASTA scores: E(): 2.3e-07, (33.3% identity in 219 aa overlap). Also similar to Rv1986 from Mycobacterium tuberculosis. P64711 No cell wall and cell processes Mb0498 NC_000962.3 Mycobrowser_v2 CDS 1485313 1485771 . - 0 Rv1322A Rv1322A Function unknown Conserved protein Rv1322A, len: 152 aa. Conserved protein, similar to proteins from Mycobacterium leprae and Streptomyces coelicolor e.g. AL583921_2|ML1157 from M. leprae strain tn (155 aa), FASTA scores: opt: 771, E(): 5.1e-43, (75.3% identity in 154 aa overlap); and AL137242_2 from Streptomyces coelicolor (146 aa), FASTA scores: opt: 404, E(): 2e-19, (43.165% identity in 139 aa overlap). Q8VK36 No conserved hypotheticals ML1157 MMAR_4076 MSMEG_4921 Mb1357c NC_000962.3 Mycobrowser_v2 CDS 578426 579175 . + 0 Rv0489 gpm1 Involved in glycolysis [catalytic activity: 1,3-diphosphoglycerate + 3-phosphoglycerate = 2,3-diphosphoglycerate + 3-phosphoglycerate]. Probable phosphoglycerate mutase 1 Gpm1 (phosphoglyceromutase) (PGAM) (BPG-dependent PGAM) Rv0489, (MTCY20G9.15), len: 249 aa. Probable gpm1, phosphoglycerate mutase 1, equivalent to P53531|PMGY_MYCLE phosphoglycerate mutase from Mycobacterium leprae (247 aa). Also highly similar to others e.g. PMG1_ECOLI|P31217 (249 aa), FASTA scores: opt: 805, E(): 0, (51.4% identity in 245 aa overlap); etc. Contains PS00175 Phosphoglycerate mutase family phosphohistidine signature, and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the phosphoglycerate mutase family. Note that previously known as gpm. P0A5R6 No intermediary metabolism and respiration ML2441 MMAR_0814 MSMEG_0935 Mb0499 NC_000962.3 Mycobrowser_v2 CDS 579349 580581 . + 0 Rv0490 senX3 Sensor part of a two component regulatory system. Probably forms part of a two-component regulatory system SENX3/REGX3; phosphorylates REGX3. Putative two component sensor histidine kinase SenX3 Rv0490, (MTCY20G9.16), len: 410 aa. Putative senX3, two-component sensor histidine kinase, transmembrane protein (see citations below), equivalent to O07129|SEX3_MYCBO sensor-like histidine kinase SENX3 from Mycobacterium bovis BCG (410 aa), FASTA scores: E(): 0, (99.5% identity in 410 aa overlap); and highly similar to P54883|SEX3_MYCLE|SENX3 sensor-like histidine kinase from Mycobacterium leprae (443 aa), FASTA score: (83.8% identity in 408 aa overlap). Also highly similar, except in N-terminus, to CAC31957.1|AL583925 probable two-component system sensor histidine kinase from Mycobacterium leprae (441 aa). Also highly similar to sensor kinase proteins from other organisms e.g. CAB77323.1|AL160331 putative sensor kinase protein from Streptomyces coelicolor (426 aa). P0A600 No regulatory proteins ML2440 MMAR_0815 MSMEG_0936 Mb0500 NC_000962.3 Mycobrowser_v2 RBS 610179 610182 . + 0 Rv0518 Rv0518 Unknown Possible exported protein Rv0518, (MTCY20G10.08), len: 231 aa. Possible exported protein; has hydrophobic N-terminus. O33363 No cell wall and cell processes MMAR_0855 MSMEG_3489 Mb0531 NC_000962.3 Mycobrowser_v2 CDS 610188 610883 . + 0 Rv0518 Rv0518 Unknown Possible exported protein Rv0518, (MTCY20G10.08), len: 231 aa. Possible exported protein; has hydrophobic N-terminus. O33363 No cell wall and cell processes MMAR_0855 MSMEG_3489 Mb0531 NC_000962.3 Mycobrowser_v2 CDS 580809 581492 . + 0 Rv0491 regX3 Transcriptional regulatory protein part of the two component regulatory system REGX3/SENX3. Two component sensory transduction protein RegX3 (transcriptional regulatory protein) (probably LuxR-family) Rv0491, (MTCY20G9.17), len: 227 aa. RegX3, response regulator protein (sensory transduction protein) (see citations below), equivalent to O07130|RGX3_MYCBO|REGX3 sensory transduction protein from Mycobacterium bovis BCG (227 aa); AAG09797.1|AF258346_2|AF258346|REGX3 response regulator from Mycobacterium smegmatis (228 aa); equivalent to P54884|RGX3_MYCLE|REGX3 sensory transduction protein from Mycobacterium leprae (198 aa), FASTA scores : E(): 0, (95.4% identity in 197 aa overlap). Also highly similar to other response regulators e.g. AAG43239.1|AF123314_2 |AF123314 putative response regulator from Corynebacterium glutamicum (232 aa). Q11156 No regulatory proteins ML2439 MMAR_0816 MSMEG_0937 Mb0501 NC_000962.3 Mycobrowser_v2 CDS 581489 583378 . - 0 Rv0492c Rv0492c Function unknown; probably involved in cellular metabolism. Probable oxidoreductase GMC-type Rv0492c, (MT0511/MT0512, MTCY20G9.18c), len: 629 aa. Probable oxidoreductase GMC type, similar to others except in N-terminus e.g. P55582|AE000087_5|Y4NJ_RHISN hypothetical GMC-type oxidoreductase from Rhizobium sp. (505 aa), FASTA scores: opt: 873, E():0, (34.3% identity in 502 aa overlap); YTH2_RHOER|P46371 hypothetical 53.0 kDa GMC-type oxidoreductase from Rhodococcus erythropolis (493 aa), FASTA score: (25.7% identity in 521 aa overlap); YTH2_RHOSO|P46371 hypothetical 53.0 kDa gmc-type oxidoreductase from Rhodococcus erythropolis (493 aa), FASTA score: (25.7% identity in 521 aa overlap); NP_085596.1|NC_002679 probable oxidoreductase from Mesorhizobium loti (507 aa); NP_285451.1|NC_001264 GMC oxidoreductase from Deinococcus radiodurans (722 aa); NP_249055.1|NC_002516 probable oxidoreductase from Pseudomonas aeruginosa (531 aa); etc. Contains PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature, and PS00624 GMC oxidoreductases signature 2. Belongs to the GMC oxidoreductases family. Cofactor: FAD (by similarity). Note that start changed since first submission (previously 684 aa). Q11157 No intermediary metabolism and respiration ML2438 MMAR_0817 MSMEG_5967 Mb0502c NC_000962.3 Mycobrowser_v2 CDS 583375 583704 . - 0 Rv0492A Rv0492A Unknown Hypothetical protein Rv0492A, len: 109 aa. Hypothetical unknown protein. GC plot suggests CDS. Q6MX36 No conserved hypotheticals MMAR_0818 Mb0503c NC_000962.3 Mycobrowser_v2 CDS 583701 584690 . - 0 Rv0493c Rv0493c Function unknown Conserved protein Rv0493c, (MTCY20G9.19), len: 329 aa. Conserved protein, showing some similarity to U00018_33|B2168_F2_93 from Mycobacterium leprae (167 aa), FASTA scores: opt: 166, E(): 0.00077, (35.9% identity in 131 aa overlap). Q11158 No conserved hypotheticals ML2437 MMAR_0819 Mb0504c NC_000962.3 Mycobrowser_v2 RBS 606543 606547 . + 0 Rv0515 Rv0515 Function unknown Conserved 13E12 repeat family protein Rv0515, (MTCY20G10.05), len: 503 aa. Part of M. tuberculosis 13E12 repeat family. Almost identical to Rv0336 (99.8% identity in 503 aa overlap), possibly due to a recent gene duplication. Also similar to other M. tuberculosis hypothetical 13E12 repeat proteins e.g. Rv1148c, Rv1945, etc. O33360 No insertion seqs and phages Mb0528 NC_000962.3 Mycobrowser_v2 CDS 606551 608062 . + 0 Rv0515 Rv0515 Function unknown Conserved 13E12 repeat family protein Rv0515, (MTCY20G10.05), len: 503 aa. Part of M. tuberculosis 13E12 repeat family. Almost identical to Rv0336 (99.8% identity in 503 aa overlap), possibly due to a recent gene duplication. Also similar to other M. tuberculosis hypothetical 13E12 repeat proteins e.g. Rv1148c, Rv1945, etc. O33360 No insertion seqs and phages Mb0528 NC_000962.3 Mycobrowser_v2 CDS 584695 585423 . + 0 Rv0494 Rv0494 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably GntR-family) Rv0494, (MTCY20G9.20), len: 242 aa. Probable transcriptional regulator, GntR family, with C-terminal part highly similar to S72893|B2168_C2_205 hypothetical protein from Mycobacterium leprae (105 aa). Also similar to other transcription regulators e.g. PDHR_ECOLI|P06957 pyruvate dehydrogenase complex repressor PDHR or GENA from Escherichia coli (254 aa), FASTA scores: opt: 284, E(): 1.2e-11, (32.6% identity in 224 aa overlap); etc. Contains PS00043 Bacterial regulatory proteins, gntR family signature, and probable helix-turn helix motif from aa 50-71 (Score 1229, +3.37 SD). P67739 No regulatory proteins ML2436 MMAR_0820 MSMEG_2173,MSMEG_2794 Mb0505 NC_000962.3 Mycobrowser_v2 CDS 587377 588309 . + 0 Rv0497 Rv0497 Unknown Probable conserved transmembrane protein Rv0497, (MTCY20G9.23), len: 310 aa. Probable conserved transmembrane protein, equivalent (but shorter in C-terminus) to P54580|Y497_MYCLE|ML2433 hypothetical 37.9 KDA protein from Mycobacterium leprae (355 aa). N-terminus highly similar to S72922|B2168_C1_166|467074 hypothetical protein from Mycobacterium leprae (118 aa), FASTA scores: opt: 350, E(): 1.4e-12, (57.9% identity in 114 aa overlap); and hydrophobic C-terminus, highly similar to S72895|B2168_C2_209|467047 hypothetical protein from Mycobacterium leprae (241 aa), FASTA scores: opt: 473, E(): 8e-19, (53.9% identity in 241 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P64715 No cell wall and cell processes ML2433 MMAR_0823 MSMEG_0940 Mb0508 NC_000962.3 Mycobrowser_v2 CDS 588325 589167 . + 0 Rv0498 Rv0498 Function unknown Conserved hypothetical protein Rv0498, (MTCY20G9.24), len: 280 aa. Conserved hypothetical protein, highly similar to P54581|Y498_MYCLE|ML2432 hypothetical 30.5 KDA protein from Mycobacterium leprae (280 aa); and S72896|B2168_C2_210 hypothetical protein from Mycobacterium leprae (244 aa), FASTA scores: opt: 1486, E():0, (89.3% identity in 244 aa overlap). Also similar to Q9X8H0|Y714_STRCO|SCE7.14c hypothetical protein from Streptomyces coelicolor. P64717 No conserved hypotheticals ML2432 MMAR_0824 MSMEG_0941 Mb0509 NC_000962.3 Mycobrowser_v2 CDS 589183 590058 . + 0 Rv0499 Rv0499 Function unknown Conserved hypothetical protein Rv0499, (MTCY20G9.25), len: 291 aa. Conserved hypothetical protein, showing some similarity to AL031184|SC2A11_16|T34762 hypothetical protein from Streptomyces coelicolor (340 aa), FASTA scores: opt: 240, E(): 1.8e-07, (28.9% identity in 270 aa overlap). P64719 No conserved hypotheticals ML2431 MMAR_0825 MSMEG_0942 Mb0510 NC_000962.3 Mycobrowser_v2 CDS 590083 590970 . + 0 Rv0500 proC Involved at the terminal (third) step in proline biosynthesis [catalytic activity: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H]. Probable pyrroline-5-carboxylate reductase ProC (P5CR) (P5C reductase) Rv0500, (MTCY20G9.26), len: 295 aa. Probable proC, Pyrroline-5-carboxylate reductase (see citation below), equivalent to P46725|PROC_MYCLE pyrroline-5-carboxylate reductase from Mycobacterium leprae (294 aa), FASTA scores: opt: 1473, E(): 0, (82.4% identity in 295 aa overlap). Also similar to others e.g. P46540|PROC_CORGL pyrroline-5-carboxylate reductase from Corynebacterium glutamicum (270 aa); T36286|4803683|CAB42663.1|AL049819 pyrroline-5-carboxylate reductase from Streptomyces coelicolor (284 aa); etc. Belongs to the pyrroline-5-carboxylate reductase family. Q11141 No intermediary metabolism and respiration ML2430 MMAR_0826 MSMEG_0943 Mb0511 NC_000962.3 Mycobrowser_v2 CDS 591111 591347 . + 0 Rv0500A Rv0500A Function unknown Conserved protein Rv0500A, len: 78 aa. Conserved protein, similar to proteins from Mycobacterium leprae and Streptomyces coelicolor e.g. U00018_25 from Mycobacterium leprae cosmid B2168 (86 aa), FASTA scores: opt: 428, E(): 1.3e-27, (82.6% identity in 86 aa overlap); AL079345|SCE68_26 from Streptomyces coelicolor cosmid E6 (70 aa), FASTA scores: opt: 252, E(): 1.2 e-13, (72.2 identity in 54 aa overlap). Q8VKK5 No conserved hypotheticals ML2429 Mb0512 NC_000962.3 Mycobrowser_v2 CDS 1733116 1733550 . - 0 Rv1532c Rv1532c Function unknown Conserved hypothetical protein Rv1532c, (MTCY07A7A.01c), len: 144 aa. Conserved hypothetical protein, similar to P20378|YPHR_HALHA Hypothetical 15.6 kDa protein from Halobacterium halobium (151 aa), FASTA scores: opt: 152, E():4.5e-05, (30.1% identity in 103 aa overlap). O06178 No conserved hypotheticals MMAR_4134 MSMEG_4984 Mb1559c NC_000962.3 Mycobrowser_v2 CDS 591654 592784 . + 0 Rv0501 galE2 Involved in galactose metabolism [catalytic activity: UDP-glucose = UDP-galactose]. Possible UDP-glucose 4-epimerase GalE2 (galactowaldenase) (UDP-galactose 4-epimerase) (uridine diphosphate galactose 4-epimerase) (uridine diphospho-galactose 4-epimerase) Rv0501, (MTCY20G9.28), len: 376 aa. Possible galE2, UDP-glucose 4-epimerase, highly similar (except in N-terminus) to CAC31944.1|AL583925 possible glucose epimerase/dehydratase from Mycobacterium leprae (364 aa). N-terminus highly similar to S72923|B2168_C1_174|467075|AAA17259.1|U00018 hypothetical protein from Mycobacterium leprae (180 aa), FASTA scores: opt: 934, E(): 0, (89.6% identity in 164 aa overlap); and C-terminus highly similar to S72898|467050|AAA17234.1|U00018 hypothetical protein from Mycobacterium leprae (168 aa), FASTA scores: opt: 928, E(): 0, (82.7% identity in 168 aa overlap). Also highly similar to T36274|5123671|CAB45360.1|AL079345 probable epimerase from Streptomyces coelicolor (353 aa); and similar in part to other epimerases e.g. GALE_ECOLI|P09147 UDP-glucose 4-epimerase from Escherichia coli (338 aa), FASTA scores: opt: 241, E(): 6.7e-09, (28.2% identity in 294 aa overlap); etc. Belongs to the sugar epimerase family. Cofactor: NAD. Note that previously known as galE1. P0A5D1 No intermediary metabolism and respiration ML2428 MMAR_0829 MSMEG_0946 Mb0513 NC_000962.3 Mycobrowser_v2 CDS 592791 593867 . + 0 Rv0502 Rv0502 Function unknown Conserved protein Rv0502, (MTCY20G9.29), len: 358 aa. Conserved protein, equivalent to P54878|Y502_MYCLE|ML2427 hypothetical 40.5 KDA protein from Mycobacterium leprae (367 aa), FASTA scores: opt: 2042, E(): 0, (84.1% identity in 365 aa overlap). Also similar to T36273|SCE68.23c hypothetical protein from Streptomyces coelicolor (355 aa). C-terminal similar to AL021529|SC10A5_4|T34572 hypothetical protein from Streptomyces coelicolor (295 aa), FASTA score: (57.8% identity in 263 aa overlap); and to hypothetical proteins from Mycobacterium tuberculosis Rv1920|G70808 (287 aa); and Rv1428c|G70914 (275 aa). P64723 No conserved hypotheticals ML2427 MMAR_0830 MSMEG_0947 Mb0514 NC_000962.3 Mycobrowser_v2 CDS 593871 594779 . - 0 Rv0503c cmaA2 Essential for the cyclopropanation function. Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge. Mycolic acids, which represent the major constituent of mycobacterial cell wall complex, act as substrates [catalytic activity: S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid]. Cyclopropane-fatty-acyl-phospholipid synthase 2 CmaA2 (cyclopropane fatty acid synthase) (CFA synthase) (cyclopropane mycolic acid synthase 2) (mycolic acid trans-cyclopropane synthetase) Rv0503c, (MTCY20G9.30c), len: 302 aa. CmaA2 (alternate gene name: cma2), cyclopropane-fatty-acyl-phospholipid synthase 2 (mycolic acid trans-cyclopropane synthetase) (see citations below). Note that this protein has 302 aa and not 322 aa: we have chosen a different initiation codon on the basis of homology. Equivalent to S72886|B2168_F3_130 hypothetical protein from Mycobacterium leprae (308 aa), FASTA score: (78.9% identity in 303 aa overlap); and highly similar to other proteins from Mycobacterium leprae. Also similar to other proteins from Mycobacterium tuberculosis and Mycobacterium bovis BCG e.g. MTV038_14|UMAA2|Rv0470c|MTV038.14 putative mycolic acid synthesis/modification protein (287 aa) (57.2% identity in 297 aa overlap). P0A5P0 No lipid metabolism ML2426 MMAR_0831 MSMEG_1205 Mb0515c NC_000962.3 Mycobrowser_v2 CDS 594802 595302 . - 0 Rv0504c Rv0504c Function unknown Conserved protein Rv0504c, (MTCY20G9.31c), len: 166 aa. Conserved protein, equivalent to P54879|Y504_MYCLE|ML2425 hypothetical 18.7 KDA protein from Mycobacterium leprae (166 aa), FASTA scores: opt: 884, E(): 0, (83.1% identity in 166 aa overlap); and highly similar to other proteins from Mycobacterium leprae. Also highly similar to CAB77410.1|AL160431|SCD82.07 hypothetical protein from Streptomyces coelicolor (150 aa). Also similar to M. tuberculosis hypothetical proteins Rv0635|H70612 (158 aa); and Rv0637|B70613 (166 aa). P64725 No conserved hypotheticals ML2425 Mb0516c NC_000962.3 Mycobrowser_v2 CDS 595464 596585 . - 0 Rv0505c serB1 Removes a phosphate from phosphoserine [catalytic activity: phosphoserine + H2O = serine + phosphate]. Possible phosphoserine phosphatase SerB1 (PSP) (O-phosphoserine phosphohydrolase) (pspase) Rv0505c, (MTCY20G9.32c), len: 373 aa. Possible serB1, phosphoserine phosphatase, equivalent (but longer ~70 aa in N-terminus) to S72914|serB phosphoserine phosphatase from Mycobacterium leprae (300 aa), FASTA scores: opt: 1570, E(): 0, (83.0% identity in 306 aa overlap). C-terminus highly similar to CAB55344.1|AJ010584 phosphoserine phosphatase from Streptomyces coelicolor (266 aa). Low similarity to SERB_ECOLI|P06862 phosphoserine phosphatase from Escherichia coli strains K12 and O157:H7 (322 aa), FASTA scores: opt: 148, E(): 0.043, (24.0% identity in 150 aa overlap). C-terminus is also similar to O33611|AB004855_1|IMD_STRCN protein involved in inhibition of morphological differentiation from Streptomyces cyaneus (277 aa), FASTA score: (37.7% identity in 252 aa overlap). Seems to belong to the SERB family. Note that previously known as serB. P66801 No intermediary metabolism and respiration ML2424 MMAR_0833 MSMEG_0949 Mb0517c NC_000962.3 Mycobrowser_v2 CDS 597199 600105 . + 0 Rv0507 mmpL2 Unknown. Thought to be involved in fatty acid transport. Probable conserved transmembrane transport protein MmpL2 Rv0507, (MTCY20G9.34), len: 968 aa. Probable mmpL2, conserved transmembrane transport protein (see citations below), member of RND superfamily, highly similar to other Mycobacterial proteins e.g. YV34_MYCLE from Mycobacterium leprae (959 aa), FASTA scores: opt: 3699, E(): 0, (58.3% identity in 940 aa overlap); and the Mycobacterium tuberculosis proteins MTV037_14, MTV040_4, MTCY98_8, MTCY4D9_15, MTCY48_8, MTCY19G5_6, MTV005_19, etc. Also similar to STMACTII_3|SC10A5_9 from Streptomyces coelicolor; and BSUB0|004_12 from Bacillus subtilis. C-terminal half similar to Q50086|U1740AB from Mycobacterium leprae (386 aa), FASTA scores: opt: 1526, E(): 0, (61.5% identity in 371 aa overlap). Belongs to the MmpL family. Q11171 No cell wall and cell processes MMAR_0840 Mb0519 NC_000962.3 Mycobrowser_v2 CDS 600441 601847 . + 0 Rv0509 hemA Involved in porphyrin biosynthesis by the C5 pathway (at the first step) [catalytic activity: glutamyl-tRNA(GLU) + NADPH = glutamate-1-semialdehyde + NADP+ + tRNA(GLU)]. Probable glutamyl-tRNA reductase HemA (GLUTR) Rv0509, (MTCY20G9.36), len: 468 aa. Probable hemA, glutamyl-tRNA reductase, equivalent to HEM1_MYCLE|P46724 glutamyl-tRNA reductase from Mycobacterium leprae (467 aa), FASTA scores: opt: 2377, E(): 0, (82.3% identity in 463 aa overlap). Also highly similar (sometimes in part) to others e.g. Q9WX15|HEM1_STRCO glutamyl-tRNA reductase from Streptomyces coelicolor (581 aa); P16618|HEM1_BACSU|HEMA glutamyl-tRNA reductase from Bacillus subtilis (455 aa); etc. Contains PS00747 Glutamyl-tRNA reductase signature. Belongs to the glutamyl-tRNA reductase family. P64328 No intermediary metabolism and respiration ML2422 MMAR_0842 MSMEG_0952 Mb0521,Mb0522 NC_000962.3 Mycobrowser_v2 CDS 4116002 4116379 . + 0 Rv3675 Rv3675 Unknown Possible membrane protein Rv3675, (MTV025.023), len: 125 aa. Possible membrane protein, with some similarity to Q9YCZ2|APE1120 hypothetical 11.7 KDA protein from Aeropyrum pernix (103 aa), FASTA scores: opt: 100, E(): 9, (40.0% identity in 55 aa overlap). O69643 No cell wall and cell processes Mb3699 NC_000962.3 Mycobrowser_v2 CDS 601857 602786 . + 0 Rv0510 hemC Involved in porphyrin biosynthesis by the C5 pathway (at the fourth step). Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane preuroporphyrinogen in several discrete steps [catalytic activity: 4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH3]. Probable porphobilinogen deaminase HemC (PBG) (hydroxymethylbilane synthase) (HMBS) (pre-uroporphyrinogen synthase) Rv0510, (MTCY21C8.01-MTCY20G9.37), len: 309 aa. Probable hemC, hydroxymethylbilane synthase (porphobilinogen deaminase), equivalent to HEM3B|Q49808|HEM3_MYCLE porphobilinogen deaminase from Mycobacterium leprae (315 aa), FASTA scores: opt: 889, E(): 0, (88.1% identity in 159 aa overlap). Also highly similar to others e.g. Q9WX16|HE31_STRCO probable porphobilinogen deaminase from Streptomyces coelicolor (319 aa); Q9L6Q2|HEM3_SALTY porphobilinogen deaminase from Salmonella typhimurium (313 aa); etc. Belongs to the HMBS family. Cofactor: covalently binds a dipyrromethane cofactor to which the porphobilinogen subunits are ADDED. P64336 No intermediary metabolism and respiration ML2421 MMAR_0843 MSMEG_0953 Mb0523 NC_000962.3 Mycobrowser_v2 CDS 611172 612074 . - 0 Rv0519c Rv0519c Unknown; could have possibly a lipolytic activity. Possible conserved membrane protein Rv0519c, (MTCY20G10.09c), len: 300 aa. Possible conserved membrane protein, with hydrophobic region near N-terminus. Could be a lipase. Similar to Rv0774c|MTCY369.19c|A70708 from Mycobacterium tuberculosis (312 aa), FASTA scores: opt: 1092, E(): 0, (57.9% identity in 299 aa overlap). Contains PS00120 Lipases, serine active site. O33364 No cell wall and cell processes ML2417 MMAR_0858 Mb0532c NC_000962.3 Mycobrowser_v2 CDS 602819 604516 . + 0 Rv0511 hemD Possibly involved in the biosynthesis of siroheme and cobalamin [catalytic activity: 2 S-adenosyl-L-methionine + uroporphyrin III = 2 S-adenosyl-L-homocysteine + sirohydrochlorin]. Probable uroporphyrin-III C-methyltransferase HemD (uroporphyrinogen III methylase) (urogen III methylase) (SUMT) (urogen III methylase) (UROM) Rv0511, (MTCY21C8.02), len: 565 aa. Probable hemD (alternate gene name: cysG), uroporphyrin-III C-methyltransferase, highly similar to others e.g. CAC31936.1|AL583925 possible uroporphyrin-III C-methyltransferase from Mycobacterium leprae (563 aa); and S72909|CYSG from Mycobacterium leprae (472 aa), FASTA scores: opt: 1946, E(): 0, (83.3% identity in 472 aa overlap); T36265|5123662|CAB45351.1|AL079345 probable uroporphyrin-III C-methyltransferase from Streptomyces coelicolor (565 aa); and similar to others e.g. AAK00606.1|AF221100_3|AF221100 from Selenomonas ruminantium subsp. ruminantium (505 aa); etc. Also similar to Rv2071c and Rv2847c from Mycobacterium tuberculosis. Note that previously known as cysG. Q6MX34 No intermediary metabolism and respiration ML2420 Mb0524 NC_000962.3 Mycobrowser_v2 RBS 3155880 3155882 . - 0 Rv0511 hemD Possibly involved in the biosynthesis of siroheme and cobalamin [catalytic activity: 2 S-adenosyl-L-methionine + uroporphyrin III = 2 S-adenosyl-L-homocysteine + sirohydrochlorin]. Probable uroporphyrin-III C-methyltransferase HemD (uroporphyrinogen III methylase) (urogen III methylase) (SUMT) (urogen III methylase) (UROM) Rv0511, (MTCY21C8.02), len: 565 aa. Probable hemD (alternate gene name: cysG), uroporphyrin-III C-methyltransferase, highly similar to others e.g. CAC31936.1|AL583925 possible uroporphyrin-III C-methyltransferase from Mycobacterium leprae (563 aa); and S72909|CYSG from Mycobacterium leprae (472 aa), FASTA scores: opt: 1946, E(): 0, (83.3% identity in 472 aa overlap); T36265|5123662|CAB45351.1|AL079345 probable uroporphyrin-III C-methyltransferase from Streptomyces coelicolor (565 aa); and similar to others e.g. AAK00606.1|AF221100_3|AF221100 from Selenomonas ruminantium subsp. ruminantium (505 aa); etc. Also similar to Rv2071c and Rv2847c from Mycobacterium tuberculosis. Note that previously known as cysG. Q6MX34 No intermediary metabolism and respiration ML2420 Mb0524 NC_000962.3 Mycobrowser_v2 CDS 1640680 1641543 . + 0 Rv1455 Rv1455 Function unknown Conserved protein Rv1455, (MTV007.02), len: 287 aa. Conserved protein, some similarity from aa 80-160 to Z99125|MLCL536.35c hypothetical Mycobacterium leprae protein (101 aa), FASTA scores: opt: 238, E(): 1.8e-08, (51.3% identity in 78 aa overlap). O53147 No conserved hypotheticals ML0586 MMAR_2260 MSMEG_3107 Mb1490 NC_000962.3 Mycobrowser_v2 CDS 604602 605591 . + 0 Rv0512 hemB Involved in porphyrin and heme biosynthesis (at the second step) [catalytic activity: 2 5-aminolevulinate = porphobilinogen + 2 H2O]. Probable delta-aminolevulinic acid dehydratase HemB (porphobilinogen synthase) (ALAD) (ALADH) Rv0512, (MTCY20G10.02), len: 329 aa. Probable hemB, delta-aminolevulinic acid dehydratase, equivalent to 46723|HEM2_MYCLE delta-aminolevulinic acid dehydratase from Mycobacterium leprae (329 aa). Also highly similar to many e.g. P54919|HEM2_STRCO from Streptomyces coelicolor (330 aa); HEM2_ECOLI|P15002 from Escherichia coli (323 aa), FASTA scores: opt: 942, E(): 0, (47.6% identity in 317 aa overlap); etc. Contains PS00169 Delta-aminolevulinic acid dehydratase active site. Belongs to the ALADH family. Cofactor: zinc. O33357 No intermediary metabolism and respiration ML2419 MMAR_0845 MSMEG_0956 Mb0525 NC_000962.3 Mycobrowser_v2 RBS 605593 605597 . + 0 Rv0513 Rv0513 Unknown Possible conserved transmembrane protein Rv0513, (MTCY20G10.03), len: 182 aa. Possible conserved transmembrane protein, with its N-terminus highly similar to S72925|B2168_C1_182 hypothetical protein from Mycobacterium leprae (103 aa), FASTA scores: opt: 217, E(): 8.2e-14, (45.3 % identity in 106 aa overlap). O33358 No cell wall and cell processes ML2418 MMAR_0846 MSMEG_0959 Mb0526 NC_000962.3 Mycobrowser_v2 CDS 605604 606152 . + 0 Rv0513 Rv0513 Unknown Possible conserved transmembrane protein Rv0513, (MTCY20G10.03), len: 182 aa. Possible conserved transmembrane protein, with its N-terminus highly similar to S72925|B2168_C1_182 hypothetical protein from Mycobacterium leprae (103 aa), FASTA scores: opt: 217, E(): 8.2e-14, (45.3 % identity in 106 aa overlap). O33358 No cell wall and cell processes ML2418 MMAR_0846 MSMEG_0959 Mb0526 NC_000962.3 Mycobrowser_v2 tRNA 4126541 4126614 . + 0 MTB000045 proY - tRNA-Pro (CGG) proY, tRNA-Pro, anticodon cgg, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v2 RBS 608733 608737 . + 0 Rv0517 Rv0517 Function unknown; probably involved in cellular metabolism. Possible membrane acyltransferase Rv0517, (MTCY20G10.07), len: 436 aa. Possible acyltransferase, integral membrane protein, equivalent (but longer 26 aa in N-terminus) to AAK44761.1|AE006954 putative acyltransferase from Mycobacterium tuberculosis strain CDC1551 (410 aa). Also similar to many acyltransferases e.g. MDMB_STRMY|Q00718 from Streptomyces mycarofaciens (387 aa), FASTA scores: opt: 200, E(): 1.1e-08, (28.2% identity in 394 aa overlap). And similar to Rv0111, Rv0228, Rv1254, Rv1565c from Mycobacterium tuberculosis. O33362 No intermediary metabolism and respiration MMAR_0854 MSMEG_3490 Mb0530 NC_000962.3 Mycobrowser_v2 CDS 608746 610056 . + 0 Rv0517 Rv0517 Function unknown; probably involved in cellular metabolism. Possible membrane acyltransferase Rv0517, (MTCY20G10.07), len: 436 aa. Possible acyltransferase, integral membrane protein, equivalent (but longer 26 aa in N-terminus) to AAK44761.1|AE006954 putative acyltransferase from Mycobacterium tuberculosis strain CDC1551 (410 aa). Also similar to many acyltransferases e.g. MDMB_STRMY|Q00718 from Streptomyces mycarofaciens (387 aa), FASTA scores: opt: 200, E(): 1.1e-08, (28.2% identity in 394 aa overlap). And similar to Rv0111, Rv0228, Rv1254, Rv1565c from Mycobacterium tuberculosis. O33362 No intermediary metabolism and respiration MMAR_0854 MSMEG_3490 Mb0530 NC_000962.3 Mycobrowser_v2 CDS 612255 612605 . + 0 Rv0520 Rv0520 Could cause methylation. Possible methyltransferase/methylase (fragment) Rv0520, (MTCY20G10.10), len: 116 aa. Possible fragment of methyltransferase (possibly first part), highly similar to part of several methyltransferases e.g. Q43445|U43683 S-adenosyl-L-methionine:DELTA24-sterol-C-methyltransferase from Glycine max (Soybean)(367 aa), FASTA scores: opt: 190, E(): 2.3e-12, (39.2% identity in 74 aa overlap). Also some similarity to MTCY19G5_5 from Mycobacterium tuberculosis. Possibly continues as Rv0521 but we can find no frameshift to account for this. O33365 No intermediary metabolism and respiration Mb0533 NC_000962.3 Mycobrowser_v2 CDS 612598 612903 . + 0 Rv0521 Rv0521 Could cause methylation Possible methyltransferase/methylase (fragment) Rv0521, (replaces MTCY20G10.11), len: 101 aa. Possible fragment of methyltransferase (possibly second part), highly similar to C-terminus of several methyltransferases e.g. AAF87203.1|AF216282 sarcosine-dimethylglycine methyltransferase from Halorhodospira halochloris (279 aa). Possibly continuation of Rv0520 but we can find no frameshift to account for this. Q8VKK2 No intermediary metabolism and respiration Mb0534 NC_000962.3 Mycobrowser_v2 CDS 613038 614342 . + 0 Rv0522 gabP Involved in 4-aminobutyrate (GABA) degradation pathway. Transporter for GABA. Responsible for the translocation of the substrate across the membrane. Probable GABA permease GabP (4-amino butyrate transport carrier) (GAMA-aminobutyrate permease) Rv0522, (MTCY20G10.12), len: 434 aa. Probable gabP, GABA permease (gamma-aminobutyrate permease), integral membrane protein, highly similar to others e.g. GABP_ECOLI|P25527 gaba permease from Escherichia coli (466 aa), FASTA scores: opt: 1218, E(): 0, (44.3% identity in 424 aa overlap); etc. Also similar to other M. tuberculosis permeases e.g. MTCY13E10.06c FASTA score: (34.4% identity in 407 aa overlap). Contains PS00218 Amino acid permeases signature. Overlaps and extends Rv0523c|MTCY25D10.01 from overlapping cosmid. Belongs to the amino acid permease family (APC family). Q8VKK1 No cell wall and cell processes ML2416 MMAR_0862 Mb0535 NC_000962.3 Mycobrowser_v2 CDS 614326 614721 . - 0 Rv0523c Rv0523c Function unknown Conserved protein Rv0523c, (MTCY25D10.02), len: 131 aa. Conserved protein, showing some similarity to M. tuberculosis proteins Rv1598c|MTCY336.06; and Rv1871c|MTCY336_06|O06592 (136 aa), FASTA scores: opt: 197, E(): 5e-08, (38.4% identity in 99 aa overlap). O06389 No conserved hypotheticals ML2415 Mb0536c NC_000962.3 Mycobrowser_v2 CDS 614835 616223 . + 0 Rv0524 hemL Involved in porphyrin biosynthesis by the C5 pathway (at the second step) [catalytic activity: (S)-4-amino-5-oxopentanoate = 5-aminolevulinate]. Probable glutamate-1-semialdehyde 2,1-aminomutase HemL (GSA) (glutamate-1-semialdehyde aminotransferase) (GSA-at) Rv0524, (MTCY25D10.03), len: 462 aa. Probable hemL, glutamate-1-semialdehyde 2,1-aminomutase, equivalent to P46716|GSA_MYCLE glutamate-1-semialdehyde 2,1-aminomutase from Mycobacterium leprae (446 aa), FASTA scores: opt: 1532, E(): 0, (82.6% identity in 460 aa overlap). Also highly similar to others e.g. Q9F2S0|GSA_STRCO from Streptomyces coelicolor (438 aa); Q06774|GSA_PROFR from Propionibacterium freudenreichii (441 aa); etc. Contains PS00600 Aminotransferases class-III pyridoxal-phosphate attachment site. Belongs to class-III of pyridoxal-phosphate-dependent aminotransferases. Cofactor: pyridoxal phosphate. P63506 No intermediary metabolism and respiration ML2414 MMAR_0870 MSMEG_0969 Mb0537 NC_000962.3 Mycobrowser_v2 CDS 622793 624577 . + 0 Rv0532 PE_PGRS6 Function unknown PE-PGRS family protein PE_PGRS6 Rv0532, (MTCY25D10.11), len: 594 aa. PE_PGRS6, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), similar to others e.g. Y0DP_MYCTU|Q50615 from Mycobacterium tuberculosis (498 aa), FASTA scores: opt: 1703, E(): 0, (58.2% identity in 536 aa overlap). Q6MX30 No PE/PPE MMAR_2097,MMAR_2596,MMAR_4149,MMAR_4561,MMAR_5339 Mb0545,Mb0546 NC_000962.3 Mycobrowser_v2 CDS 616223 616831 . + 0 Rv0525 Rv0525 Function unknown Conserved protein Rv0525, (MTCY25D10.04), len: 202 aa. Conserved protein, equivalent to Q49821|B2168_C3_276|S72912 hypothetical protein from Mycobacterium leprae (202 aa), FASTA scores: opt: 1151, E(): 0, (82.5% identity in 200 aa overlap). Also highly similar to CAC08377.1|AL392176 putative phosphoglycerate mutase from Streptomyces coelicolor (233 aa); and similar to SLL0395|Q55734 hypothetical 23.8 kDa protein from synechocystis SP. (212 aa), FASTA scores: opt: 207, E(): 5.1e-07, (28.2% identity in 195 aa overlap). Also some similarity to Rv2228c|Y019_MYCTU|Q10512|cy427.09 hypothetical 39.2 kDa protein from Mycobacterium tuberculosis (364 aa), FASTA scores: opt: 236, E(): 1.1e-08, (34.3% identity in 198 aa overlap). O06391 No conserved hypotheticals ML2413 MMAR_0871 MSMEG_0970 Mb0538 NC_000962.3 Mycobrowser_v2 CDS 616846 617496 . + 0 Rv0526 Rv0526 Possibly acts on thioredoxin Possible thioredoxin protein (thiol-disulfide interchange protein) Rv0526, (MTCY25D10.05), len: 216 aa. Possible thioredoxin protein (thiol-disulfide interchange protein) , equivalent to Q49816|U2168C|S72901 hypothetical protein from Mycobacterium leprae (216 aa), FASTA scores: opt: 1144, E(): 0, (78.5% identity in 214 aa overlap). C-terminus shows some similarity to C-terminus of thioredoxins e.g. RESA_BACSU|P35160 resa protein from Bacillus subtilis (181 aa), FASTA scores: opt: 200, E(): 7.4e-06, (24.2% identity in 132 aa overlap); etc. Also similar to Mycobacterium tuberculosis thioredoxin-like proteins Rv1470, Rv1471, Rv1677, etc. Contains PS00194 Thioredoxin family active site. Seems to belong to the thioredoxin family. O06392 No intermediary metabolism and respiration ML2412 MMAR_0872 MSMEG_0971 Mb0539 NC_000962.3 Mycobrowser_v2 CDS 617493 618272 . + 0 Rv0527 ccdA Possibly involved in cytochrome C synthesis. Might transfer reducing equivalents across the cytoplasmic membrane, promoting efficient disulfide bond isomerization of proteins localized on the outer surface of the membrane. Possible cytochrome C-type biogenesis protein CcdA Rv0527, (MTCY25D10.06), len: 259 aa. Possible ccdA, cytochrome C-type biogenesis protein, integral membrane protein, equivalent to Q49810|B2168_C1_192|S72890 hypothetical protein from Mycobacterium leprae (262 aa), FASTA scores: opt: 1341, E(): 0, (79.0% identity in 262 aa overlap). Also highly similar to others e.g. CAC08380.1 (253 aa); CCDA_BACSU|P45706 cytochrome C-type biogenesis protein from Bacillus subtilis (235 aa), FASTA scores: opt: 307, E(): 7.4e-13, (30.4% identity in 237 aa overlap); etc. Seems to belong to the DSBD subfamily. Note that previously known as ccsA. Q7D9P9 No intermediary metabolism and respiration ML2411 MMAR_0873 MSMEG_0972 Mb0540 NC_000962.3 Mycobrowser_v2 CDS 779543 780040 . - 0 Rv0679c Rv0679c Function unknown Conserved threonine rich protein Rv0679c, (MTV040.07c), len: 165 aa. Conserved Thr-rich protein, similar in part to neighboring ORF Rv0680c (124 aa), FASTA score: (35.1% identity in 131 aa overlap); and Rv0314c (220 aa). Predicted to be an outer membrane protein (See Song et al., 2008). O53787 No conserved hypotheticals ML1884 MMAR_1008 MSMEG_1393 Mb0698c NC_000962.3 Mycobrowser_v2 CDS 650779 651504 . - 0 Rv0560c Rv0560c Possibly causes methylation Possible benzoquinone methyltransferase (methylase) Rv0560c, (MTCY25D10.39c), len: 241 aa. Possible benzoquinone methyltransferase (see citation below), similar to other hypothetical proteins and methyltransferases e.g. Q54300 methyltransferase (211 aa), FASTA scores: opt: 203, E(): 4.8e-07, (30.9% identity in 136 aa overlap). Similar to Rv3699, Rv1377c, Rv2675c, etc from Mycobacterium tuberculosis. Rv0560c can be induced by salicylate and para-amino-salicylate (pas). O06426 No intermediary metabolism and respiration ML2275 MMAR_0909 MSMEG_2539 Mb0575c NC_000962.3 Mycobrowser_v2 CDS 618305 619894 . + 0 Rv0528 Rv0528 Unknown Probable conserved transmembrane protein Rv0528, (MTCY25D10.07), len: 529 aa. Probable conserved transmembrane protein, equivalent (shorter 14 aa in N-terminus) to CAC31926.1|AL583925 conserved membrane protein from Mycobacterium leprae (542 aa). Also highly similar to Q49817|B2168_C2_237|S72902 hypothetical protein from Mycobacterium leprae (364 aa), FASTA scores: opt: 1846, E(): 0, (81.1% identity in 338 aa overlap); and Q49811|B2168_C1_194|S72891 hypothetical protein from Mycobacterium leprae (106 aa), FASTA scores: opt: 506, E(): 3.8e-26, (73.6% identity in 106 aa overlap). Also highly similar to CAC08381.1|AL392176 putative integral membrane protein from Streptomyces coelicolor (574 aa). O06394 No cell wall and cell processes ML2410 MMAR_0874 MSMEG_0973 Mb0541 NC_000962.3 Mycobrowser_v2 CDS 1241971 1242831 . - 0 Rv1118c Rv1118c Function unknown Conserved protein Rv1118c, (MTCY22G8.07c), len: 286 aa. Conserved protein, similar to pseudogene ML0942 in Mycobacterium leprae. O06570 No conserved hypotheticals ML0942 MMAR_4342 MSMEG_5213 Mb1149c NC_000962.3 Mycobrowser_v2 CDS 619891 620865 . + 0 Rv0529 ccsA Required during cytochrome biogenesis at the step of heme attachment. Possible cytochrome C-type biogenesis protein CcsA Rv0529, (MTCY25D10.08), len: 324 aa. Possible ccsA, cytochrome C-type biogenesis protein, integral membrane protein, equivalent to NP_302558.1|NC_002677|B2168_C3_281 possible cytochrome C biogenesis protein from Mycobacterium leprae (327 aa), FASTA scores: opt: 1779, E(): 0, (82.9% identity in 327 aa overlap). Also highly similar to others e.g. CAC08382.1|AL392176 putative cytochrome biogenesis related protein from Streptomyces coelicolor (380 aa); CCSA_CHLRE|P48269 probable cytochrome c biogenesis protein from Chlamydomonas reinhardtii (353 aa), FASTA scores: opt: 449, E(): 1.3e-23, (34.4% identity in 247 aa overlap); etc. Belongs to the CCMF/CYCK/CCL1/NRFE/CCSA family. Note that previously known as ccsB. O06393 No intermediary metabolism and respiration ML2409 MMAR_0875 MSMEG_0974 Mb0542 NC_000962.3 Mycobrowser_v2 CDS 620907 622124 . + 0 Rv0530 Rv0530 Function unknown Conserved protein Rv0530, (MTCY25D10.09), len: 405 aa. Conserved protein, similar in part to other hypothetical proteins e.g. AL031231|SC3C3_3|CAA20252.1 from Streptomyces coelicolor (1083 aa), FASTA scores: opt: 870, E(): 0, (39.5% identity in 443 aa overlap); etc. Also similar to Mycobacterium tuberculosis proteins e.g. Rv3868, Rv0282, Rv1798, etc. O06396 No conserved hypotheticals ML2408 MMAR_0876 MSMEG_0975 Mb0543 NC_000962.3 Mycobrowser_v2 CDS 622121 622282 . - 0 Rv0530A Rv0530A Unknown Conserved protein Rv0530A, len: 53 aa. Conserved protein. No conserved hypotheticals NC_000962.3 Mycobrowser_v2 CDS 622329 622646 . + 0 Rv0531 Rv0531 Unknown Possible conserved membrane protein Rv0531, (MTCY25D10.10), len: 105 aa. Possible conserved membrane protein, highly similar to Y13803|MLB1306_1|CAA74131.1 hypothetical protein from Mycobacterium leprae (86 aa), FASTA scores: E(): 2.1e-24, (74.4% identity in 86 aa overlap); and NP_302557.1|NC_002677 putative membrane protein from Mycobacterium leprae (111 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06397 No cell wall and cell processes ML2407 MMAR_0878 MSMEG_0977 Mb0544 NC_000962.3 Mycobrowser_v2 CDS 697154 697567 . - 0 Rv0598c vapC27 Unknown Possible toxin VapC27. Contains PIN domain. Rv0598c, (MTCY19H5.24), len: 137 aa. Possible vapC27, toxin, part of toxin-antitoxin (TA) operon with Rv00599c, contains PIN domain, see Arcus et al. 2005. Similar to others e.g. Rv2596|Y0B5_MYCTU|Q50625 conserved hypothetical protein from Mycobacterium tuberculosis (134 aa), FASTA scores: opt: 254, E(): 8.2e-12, (41.5% identity in 130 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07780 No virulence, detoxification, adaptation Mb0614c NC_000962.3 Mycobrowser_v2 CDS 624473 625480 . - 0 Rv0533c fabH Involved in fatty acid biosynthesis. Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. KAS III catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities [catalytic activity: acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]]. 3-oxoacyl-[acyl-carrier-protein] synthase III FabH (beta-ketoacyl-ACP synthase III) (KAS III) Rv0533c, (MTCY25D10.12c), len: 335 aa. FabH (alternate gene name: mtFabH), 3-oxoacyl-[acyl-carrier protein] synthase III (see citations below), highly similar to others e.g. Q54206|FABH from streptomyces glaucescens (333 aa), FASTA scores: opt: 1109, E(): 0, (51.4% identity in 333 aa overlap); FABH_ECOLI|P24249 3-oxoacyl-[acyl-carrier-protein] synthase III (317 aa), FASTA scores: opt: 666, E(): 0, (37.1% identity in 318 aa overlap); etc. Belongs to the FabH family. P0A574 No lipid metabolism MMAR_0879 Mb0547c NC_000962.3 Mycobrowser_v2 CDS 1264606 1264947 . + 0 Rv1136 Rv1136 Involved in carnitine metabolism. Possible enoyl-CoA hydratase Rv1136, (MTCI65.03), len: 113 aa. Probable enoyl-CoA hydratase (possible gene fragment). Some similarity to N-terminus of carnitine racemases and enoyl-CoA hydratases (but much shorter) e.g. I41014 carnitine racemase from Escherichia coli (297 aa), FASTA scores: opt: 258, E(): 2.5e-11, (44.5% identity in 110 aa overlap); and Rv0222 putative enoyl-CoA hydratase from M. tuberculosis (262 aa). O06536 No lipid metabolism Mb1168 NC_000962.3 Mycobrowser_v2 CDS 633055 634404 . - 0 Rv0541c Rv0541c Unknown Probable conserved integral membrane protein Rv0541c, (MTCY25D10.20c), len: 449 aa. Probable conserved integral membrane protein, highly similar (except first 40 residues) to CAB76994.1|AL159178 putative integral membrane protein from Streptomyces coelicolor (456 aa). Also some similarity to Q13724|GCS1_HUMAN mannosyl-oligosaccharide glucosidase (834 aa), FASTA scores: opt: 150, E(): 0.013, (27.1% identity in 339 aa overlap). Contains PS00041 Bacterial regulatory proteins, araC family signature. O06407 No cell wall and cell processes ML2256 MMAR_0887 MSMEG_0999 Mb0555c NC_000962.3 Mycobrowser_v2 CDS 1242864 1243013 . - 0 Rv1119c Rv1119c Unknown Hypothetical protein Rv1119c, (MTCY22G8.08c), len: 49 aa. Hypothetical unknown protein. O06571 No conserved hypotheticals Mb1150c NC_000962.3 Mycobrowser_v2 CDS 625562 626440 . - 0 Rv0534c menA Involved in menaquinone biosynthesis. Conversion of 1,4-dihydroxy-2-naphthoate (DHNA) to dimethylmenaquinone (DMK). Attaches octaprenylpyrophosphate, a membrane-bound 40-carbon side chain to DHNA. The conversion of DHNA to DMK proceeds in three stages: the removal of the carboxyl group of DHNA as CO2, the attachment of the isoprenoid side chain, and a quinol-to-quinone oxidation, which is thought to be spontaneous. 1,4-dihydroxy-2-naphthoate octaprenyltransferase MenA (DHNA-octaprenyltransferase) Rv0534c, (MTCY25D10.13c), len: 292 aa. Probable menA, 1,4-dihydroxy-2-naphthoate octaprenyltransferase, integral membrane protein, equivalent to Y13803|MLB1306_2|NP_302556.1 probable 4-dihydroxy-2-naphthoate octaprenyltransferase from Mycobacterium leprae (294 aa), FASTA scores: opt: 1509, E(): 0, (80.2% identity in 288 aa overlap). Also highly similar to others e.g. MENA_ECOLI|P32166|B3930 from Escherichia coli (308 aa), FASTA scores: opt: 495, E(): 2.9e-25, (36.3 identity in 289 aa overlap); etc. Belongs to the MenA family. P65650 No intermediary metabolism and respiration ML2406 MMAR_0880 MSMEG_0988 Mb0548c NC_000962.3 Mycobrowser_v2 CDS 626457 627251 . + 0 Rv0535 pnp Phosphorylates 5'-methylthioadenosin [catalytic activity: 5'-methylthioadenosine + phosphate = adenine + 5-methylthio-D-ribose 1-phosphate]. Probable 5'-methylthioadenosine phosphorylase Pnp (MTA phosphorylase) Rv0535, (MTCY25D10.14c), len: 264 aa. Probable pnp, 5'-methylthioadenosine phosphorylase, highly similar to others e.g. CAB90972.1|AL355832 putative methylthioadenosine phosphorylase from Streptomyces coelicolor (280 aa); etc. Also similar to Rv3307|deoD probable purine nucleoside phosphorylase from Mycobacterium tuberculosis (268 aa). Belongs to the PNP/MTAP family 2 of phosphorylases. Gene name could be inappropriate. O06401 No intermediary metabolism and respiration ML2405 MMAR_0881 MSMEG_0990 Mb0549 NC_000962.3 Mycobrowser_v2 CDS 627248 628288 . + 0 Rv0536 galE3 Possibly involved in galactose metabolism [catalytic activity: UDP-glucose = UDP-galactose]. Probable UDP-glucose 4-epimerase GalE3 (galactowaldenase) (UDP-galactose 4-epimerase) (uridine diphosphate galactose 4-epimerase) (uridine diphospho-galactose 4-epimerase) Rv0536, (MTCY25D10.15), len: 346 aa. Possible galE3, UDP-glucose 4-epimerase, highly similar to CAB76986.1|AL159178 putative epimerase from Streptomyces coelicolor (334 aa); and similar to other epimerases e.g. NP_436775.1|NC_003078 putative NDP-glucose dehydrataseepimerase protein from Sinorhizobium meliloti (368 aa); AF143772|AF143772_7 GepiA from Mycobacterium avium strain 2151 (353 aa), FASTA scores: opt: 577, E(): 3.9e-29, (36.6% identity in 352 aa overlap); GALE_METJA|Q57664 putative UDP-glucose 4-epimerase (305 aa), FASTA scores: opt: 300, E(): 1.6e-12, (30.9% identity in 343 aa overlap); etc. Also similar to Mycobacterium tuberculosis proteins e.g. Rv3634c, Rv3784, etc. Seems to belong to the sugar epimerase family. Note that previously known as galE2. Q7D9P4 No intermediary metabolism and respiration ML2404 MMAR_0882 MSMEG_0991 Mb0550 NC_000962.3 Mycobrowser_v2 CDS 628298 629731 . - 0 Rv0537c Rv0537c Unknown Probable integral membrane protein Rv0537c, (MTCY25D10.16c), len: 477 aa. Probable integral membrane protein, showing weak similarity to YDNK_STRCO|P40180 hypothetical 41.2 kDa protein from Streptomyces coelicolor (411 aa), FASTA scores: opt: 122, E(): 0.85, (28.2% identity in 373 aa overlap). O06403 No cell wall and cell processes ML0305 MMAR_0883 MSMEG_1189 Mb0551c NC_000962.3 Mycobrowser_v2 CDS 630040 631686 . + 0 Rv0538 Rv0538 Unknown Possible conserved membrane protein Rv0538, (MTCY25D10.17), len: 548 aa. Possible conserved membrane protein. Middle region highly similar to AAB63811.1|AF009829|MBE4863a|O32850 unknown protein from Mycobacterium bovis (295 aa) possible transmembrane protein with a repetitive proline, threonine-rich region at C-terminus. O06404 No cell wall and cell processes ML0306 MMAR_0884 MSMEG_1188 Mb0552 NC_000962.3 Mycobrowser_v2 CDS 631743 632375 . + 0 Rv0539 Rv0539 Substrate (sugar) unknown [catalytic activity: NDP-sugar + dolichyl phosphate = NDP + dolichyl sugar phosphate]. Probable dolichyl-phosphate sugar synthase (dolichol-phosphate sugar synthetase) (dolichol-phosphate sugar transferase) (sugar phosphoryldolichol synthase) Rv0539, (MTCY25D10.18), len: 210 aa. Probable dolichol-P-sugar synthase, highly similar to CAB76989.1|AL159178 putative glycosyltransferase from Streptomyces coelicolor (242 aa), and similar to various dolichol-P-sugar synthetases and sugar transferases e.g. NP_126257.1|NC_000868 dolichyl-phosphate mannose synthase related protein from Pyrococcus abyssi (211 aa); N-terminus of NP_127133.1|NC_000868 dolichol-P-glucose synthetase from Pyrococcus abyssi (378 aa); N-terminus of NP_068880.1|NC_000917 putative dolichol-P-glucose synthetase from Archaeoglobus fulgidus (369 aa), FASTA scores: E(): 2.4e-13, (32. 1% identity in 193 aa overlap); Q26732 dolichyl-phosphate-mannose synthase precursor from trypanosoma brucei (267 aa), FASTA scores: opt: 179, E(): 0.0011, (30.7% identity in 205 aa overlap); etc. Also similar to Rv2051c|MTY25D10_18 from Mycobacterium tuberculosis. Contains PS00017 ATP/GTP-binding site motif A (P-loop). P0A597 No intermediary metabolism and respiration MMAR_0885 MSMEG_0995 Mb0553 NC_000962.3 Mycobrowser_v2 CDS 1530173 1530925 . + 0 Rv1359 Rv1359 Involved in transcriptional mechanism Probable transcriptional regulatory protein Rv1359, (MTCY02B10.23), len: 250 aa. Probable transcriptional regulatory protein, similar to Rv0891c|MTCY31.19c, (48.5% identity in 204 aa overlap) and to Rv1358|MTCY02B10.22 (43.7% identity in 197 aa overlap). Q11029 No regulatory proteins Mb1394 NC_000962.3 Mycobrowser_v2 CDS 1743778 1744371 . - 0 Rv1541c lprI Unknown Possible lipoprotein LprI Rv1541c, (MTCY48.24), len: 197 aa. Possible lipoprotein lprI, contains appropriately positioned prokaryotic membrane lipoprotein lipid attachment site (PS0013). P65318 No cell wall and cell processes ML1202 MMAR_2364 Mb1568c NC_000962.3 Mycobrowser_v2 CDS 634416 635504 . - 0 Rv0542c menE Involved in menaquinone biosynthesis. O-succinylbenzoic acid (OSB) to O-succinylbenzoyl-CoA (OSB-CoA) [catalytic activity: ATP + O-succinylbenzoate + CoA = AMP + diphosphate + O-succinylbenzoyl-CoA]. Possible O-succinylbenzoic acid--CoA ligase MenE (OSB-CoA synthetase) (O-succinylbenzoate-CoA synthase) Rv0542c, (MTCY25D10.21c), len: 362 aa. Possible menE, O-succinylbenzoic acid-CoA ligase, highly similar to Q50170|AAA63145.1|U15187|XCLB 4-Coumarate--CoA ligase from Mycobacterium leprae (352 aa), FASTA scores: opt: 1815, E(): 0, (78.9% identity in 351 aa overlap). Also similar to N-terminus of acid-CoA ligases e.g. NP_471116.1|NC_003212 O-succinylbenzoic acid-CoA ligase from Listeria innocua (469 aa); NP_390957.1|NC_000964 O-succinylbenzoic acid-CoA ligase from Bacillus subtilis (486 aa); MENE_HAEIN|P44565 O-succinylbenzoic acid-CoA ligase from Haemophilus influenzae (452 aa), FASTA scores: opt: 307, E(): 4.6e-12, (25.4% identity in 339 aa overlap); etc. Also some similarity with fadD proteins from Mycobacterium tuberculosis. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family. O06408 No intermediary metabolism and respiration ML2257 MMAR_0888 MSMEG_1062 Mb0556c NC_000962.3 Mycobrowser_v2 CDS 635573 635875 . - 0 Rv0543c Rv0543c Function unknown Conserved protein Rv0543c, (MTCY25D10.22c), len: 100 aa. Conserved protein, equivalent to Q50171|MLU15187_32|NP_302469.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (100 aa), FASTA scores: opt: 493, E(): 6.1e-30, (73.5% identity in 98 aa overlap). Some similarity to Rv3046c|NP_217562.1 from Mycobacterium tuberculosis. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Alternative nucleotide at position 635633 (C->T; A81A) has been observed. O06409 No conserved hypotheticals ML2258 MMAR_0889 MSMEG_1063 Mb0557c NC_000962.3 Mycobrowser_v2 CDS 635935 636213 . - 0 Rv0544c Rv0544c Unknown Possible conserved transmembrane protein Rv0544c, (MTCY25D10.23c), len: 92 aa. Possible conserved transmembrane protein, equivalent to NP_302470.1|NC_002677 possible membrane protein from Mycobacterium leprae (96 aa); and shows some similarity to MLU15187_33|Q50172|U296V from Mycobacterium leprae (36 aa), FASTA scores: opt: 151, E(): 2.1e-05, (71.4% identity in 35 aa overlap). Also some similarity with VATL_NEPNO|Q26250 vacuolar ATP synthase 16 kDa proteolipid from Nephrops norvegicus (159 aa), FASTA scores: opt: 80, E(): 11, (26.1% identity in 88 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06410 No cell wall and cell processes ML2259 MMAR_0890 MSMEG_1065 Mb0558c NC_000962.3 Mycobrowser_v2 CDS 636210 637463 . - 0 Rv0545c pitA Involved in low-affinity inorganic phosphate transport across the membrane. Responsible for the translocation of the substrate across the membrane. Probable low-affinity inorganic phosphate transporter integral membrane protein PitA Rv0545c, (MTCY25D10.24c), len: 417 aa. Probable pitA, low-affinity inorganic phosphate transporter, integral membrane protein, equivalent to Q50173|NP_302471.1 pitA from Mycobacterium leprae (414 aa), FASTA scores: opt: 2035, E(): 0, (76.3% identity in 418 aa overlap). Also highly similar to others e.g. CAB59461.1|AL132644 putative low-affinity phosphate transport protein from Streptomyces coelicolor (423 aa); PITA_ECOLI|P37308 low-affinity inorganic phosphate transporter from Escherichia coli (499 aa), FASTA scores: opt: 304, E(): 6.9e-10, (32.5 % identity in 234 aa overlap); etc. Belongs to the PHO-4 family of transporters, pit subfamily. O06411 No cell wall and cell processes ML2260 MMAR_0891 MSMEG_1064 Mb0559c NC_000962.3 Mycobrowser_v2 CDS 637583 637969 . - 0 Rv0546c Rv0546c Function unknown Conserved protein Rv0546c, (MTCY25D10.25c), len: 128 aa. Conserved protein, equivalent to AAA63111.1|U15187|Q50174|U296X hypothetical protein from Mycobacterium leprae (144 aa), FASTA scores: opt: 748, E(): 0, (84.2% identity in 133 aa overlap). Also highly similar to CAB95979.1|AL360034 conserved hypothetical protein from Streptomyces coelicolor (130 aa); and similar to AE000854_8|O26852 S-D-lactoylglutathione methylglyoxal lyase from Methanobacterium thermoautotropto (116 aa), FASTA scores: opt: 155, E(): 0.00019, (30.6% identity in 108 aa overlap); YAER_ECOLI hypothetical 14.7 kDa protein from Escherichia coli (129 aa), FASTA scores: opt: 104, E(): 0.42, (28.7% identity in 115 aa overlap). Also similar to Rv2068c from Mycobacterium tuberculosis. O06412 No conserved hypotheticals ML2261 MMAR_0892 MSMEG_1067 Mb0560c NC_000962.3 Mycobrowser_v2 RBS 658309 658313 . + 0 Rv0567 Rv0567 Causes methylation Probable methyltransferase/methylase Rv0567, (MTV039.05), len: 339 aa. Probable methyltransferase, similar to several e.g. P39896|TCMO_STRGA tetracenomycin polyketide synthesis 8-O-methyltransferase from Streptomyces glaucescens (339 aa), FASTA scores: opt: 685, E(): 0, (35.8% identity in 335 aa overlap); P10950|HIOM_BOVIN hydroxyindole O-methyltransferase from Bos taurus (345 aa), FASTA scores: opt: 509, E(): 3.4e-27, (30.7% identity in 332 aa overlap) etc. O53764 No intermediary metabolism and respiration MMAR_0934 Mb0582 NC_000962.3 Mycobrowser_v2 CDS 658321 659340 . + 0 Rv0567 Rv0567 Causes methylation Probable methyltransferase/methylase Rv0567, (MTV039.05), len: 339 aa. Probable methyltransferase, similar to several e.g. P39896|TCMO_STRGA tetracenomycin polyketide synthesis 8-O-methyltransferase from Streptomyces glaucescens (339 aa), FASTA scores: opt: 685, E(): 0, (35.8% identity in 335 aa overlap); P10950|HIOM_BOVIN hydroxyindole O-methyltransferase from Bos taurus (345 aa), FASTA scores: opt: 509, E(): 3.4e-27, (30.7% identity in 332 aa overlap) etc. O53764 No intermediary metabolism and respiration MMAR_0934 Mb0582 NC_000962.3 Mycobrowser_v2 CDS 638032 638916 . - 0 Rv0547c Rv0547c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0547c, (MTCY25D10.26c), len: 294 aa. Possible oxidoreductase, similar to various oxidoreductases e.g. fatty acyl-CoA reductase from Acinetobacter calcoaceticus (295 aa); NP_280196.1|NC_002607 3-oxoacyl-[acyl-carrier-protein] reductase from Halobacterium sp. NRC-1 (255 aa); NP_349214.1|NC_003030 Short-chain alcohol dehydrogenase family protein from Clostridium acetobutylicum (255 aa); etc. Also similar to several proteins from Mycobacterium tuberculosis e.g. Y04M_MYCTU|Q10783 putative oxidoreductase (341 aa), FASTA scores: opt: 644, E(): 0, (46.1% identity in 258 aa overlap). O06413 No intermediary metabolism and respiration ML2262 MMAR_0894 MSMEG_1073 Mb0561c NC_000962.3 Mycobrowser_v2 CDS 639012 639956 . - 0 Rv0548c menB Involved in menaquinone biosynthesis. Convert O-succinylbenzoyl-CoA (OSB-CoA) to 1,4-dihydroxy-2-naphthoic acid (DHNA) [catalytic activity: O-succinylbenzoyl-CoA = 1,4-dihydroxy-2-naphthoate + CoA]. Naphthoate synthase MenB (dihydroxynaphthoic acid synthetase) (DHNA synthetase) Rv0548c, (MTCY25D10.27c), len: 314 aa. menB, naphthoate synthase (dihydroxynaphthonic acid synthase), equivalent to NP_302473.1|NC_002677 naphthoate synthase from Mycobacterium leprae (300 aa). Also similar to others e.g. MENB_ECOLI|P27290 naphthoate synthase from Escherichia coli (285 aa), FASTA scores: opt: 599, E(): 9.3e-33, (48.1 identity in 285 aa overlap); etc. Belongs to the enoyl-CoA hydratase/isomerase family. O06414 No intermediary metabolism and respiration ML2263 MMAR_0895 MSMEG_1075 Mb0562c NC_000962.3 Mycobrowser_v2 CDS 640228 640641 . - 0 Rv0549c vapC3 Unknown Possible toxin VapC3 Rv0549c, (MTCY25D10.28c), len: 137 aa. Possible vapC3, toxin, part of toxin-antitoxin (TA) operon with Rv0550c, contains PIN domain (see Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others e.g. Rv0960, Rv0065, and Rv1720c from Mycobacterium tuberculosis. O06415 No virulence, detoxification, adaptation Mb0563c,Mb0564c NC_000962.3 Mycobrowser_v2 CDS 640638 640904 . - 0 Rv0550c vapB3 Unknown Possible antitoxin VapB3 Rv0550c, (MTCY25D10.29c), len: 88 aa. Possible vapB3, antitoxin, part of toxin-antitoxin (TA) operon with Rv0549c (See Arcus et al., 2005; Pandey and Gerdes, 2005). O06416 No virulence, detoxification, adaptation Mb0565c NC_000962.3 Mycobrowser_v2 CDS 641096 642811 . - 0 Rv0551c fadD8 Function unknown, but involved in lipid degradation. Probable fatty-acid-CoA ligase FadD8 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv0551c, (MTCY25D10.30c), len: 571 aa. Probable fadD8, fatty-acid-CoA synthetase, similar to many e.g. LCFA_ECOLI|P29212 long-chain-fatty-acid--CoA ligase (561 aa), FASTA scores: opt: 585, E(): 9.5e-30, (28.7% identity in 536 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Note other possible start sites exist downstream of this start. O06417 No lipid metabolism ML2266 MMAR_0897 MSMEG_1098 Mb0566c NC_000962.3 Mycobrowser_v2 CDS 642889 644493 . + 0 Rv0552 Rv0552 Function unknown Conserved protein Rv0552, (MTCY25D10.31), len: 534 aa. Conserved protein, similar to others from several organisms. Also shows some similarity with regulatory proteins e.g. AEPA_ERWCA|Q06555 exoenzymes regulatory protein aepA [Precursor] from Erwinia carotovora (465 aa), FASTA scores: opt: 278, E(): 7.6e-11, (23.0% identity in 408 aa overlap). Also similar to Z99119|BSUB0016_28 from Bacillus subtilis (529 aa), FASTA scores: opt: 436, E(): 8.3e-20, (23.8% identity in 547 aa overlap). C-terminus is similar to MLRRNOPR_1 hypothetical 17.7 kDa protein from Mycobacterium leprae (154 aa), FASTA score: (43.1% identity in 160 aa overlap). O06418 No conserved hypotheticals ML2267 Mb0567 NC_000962.3 Mycobrowser_v2 CDS 644490 645470 . + 0 Rv0553 menC Possibly involved in menaquinone biosynthesis. Catalyzes a syn cycloisomerization [catalytic activity: 2,5-dihydro-5-oxofuran-2-acetate = cis,cis-hexadienedioate]. Probable muconate cycloisomerase MenC (cis,cis-muconate lactonizing enzyme) (MLE) Rv0553, (MTCY25D10.32), len: 326 aa. Probable menC, muconate cycloisomerase, equivalent to NP_302476.1|NC_002677 putative isomerase/racemase from Mycobacterium leprae (334 aa). Also similar to other muconate cycloisomerases e.g. TCBD_PSESP|P27099 chloromuconate cycloisomerase (370 aa), FASTA scores: opt: 249, E(): 7.8e-09, (32.7% identity in 199 aa overlap). Also similar to O-succinylbenzoate-CoA synthases. Belongs to the mandelate racemase / muconate lactonizing enzyme family. P65425 No intermediary metabolism and respiration ML2268 MMAR_0899 MSMEG_1103 Mb0568 NC_000962.3 Mycobrowser_v2 CDS 677125 677616 . - 0 Rv0580c Rv0580c Function unknown Conserved protein Rv0580c, (MTV039.18c), len: 163 aa. Conserved protein, equivalent to AAA90989.1|U20446|MK35 lipoprotein precursor from Mycobacterium kansasii (225 aa). O53777 No conserved hypotheticals ML1916 Mb0595c NC_000962.3 Mycobrowser_v2 CDS 1265087 1265455 . - 0 Rv1137c Rv1137c Unknown Hypothetical protein Rv1137c, (MTCI65.04c), len: 122 aa. Hypothetical unknown protein. O06537 No conserved hypotheticals Mb1169c NC_000962.3 Mycobrowser_v2 RBS 1265460 1265463 . - 0 Rv1137c Rv1137c Unknown Hypothetical protein Rv1137c, (MTCI65.04c), len: 122 aa. Hypothetical unknown protein. O06537 No conserved hypotheticals Mb1169c NC_000962.3 Mycobrowser_v2 CDS 651529 652755 . - 0 Rv0561c Rv0561c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0561c, (MTCY25D10.40c), len: 408 aa. Possible oxidoreductase, highly similar (except in first 30 aa) to NP_302482.1|NC_002677 putative FAD-linked oxidoreductase from Mycobacterium leprae (408 aa). Also similar to T34627 probable electron transfer oxidoreductase from Streptomyces coelicolor (430 aa); and some bacteriochlorophyll synthases e.g. NP_069300.1|NC_000917 bacteriochlorophyll synthase from Archaeoglobus fulgidus (410 aa); Q55087 geranylgeranyl hydrogenase (407 aa), FASTA scores: opt: 208, E(): 1.7e-06, (26.9% identity in 327 aa overlap). O06427 No intermediary metabolism and respiration ML2276 MMAR_0910 MSMEG_1132 Mb0576c NC_000962.3 Mycobrowser_v2 CDS 645467 646255 . + 0 Rv0554 bpoC Supposedly involved in detoxification reactions. Possible peroxidase BpoC (non-haem peroxidase) Rv0554, (MTCY25D10.33), len: 262 aa. Possible bpoC, peroxidase (non-haem peroxidase), equivalent to NP_302477.1|NC_002677 putative hydrolase from Mycobacterium leprae (265 aa). Also highly similar or similar to various hydrolases and peroxidases e.g. CAB38877.1|AL035707|T36181 probable hydrolase from Streptomyces coelicolor (272 aa); CAC48368.1|Y16952 putative hydrolase from Amycolatopsis mediterranei (284 aa); P29715|BPA2_STRAU non-haem bromoperoxidase bpo-a2 (bromide peroxidase) from Streptomyces aureofaciens (277 aa), FASTA scores: opt: 325, E(): 2.3e-15, (29.5% identity in 268 aa overlap); O31168|PRXC_STRAU|CPO|CPOT non-heme chloroperoxidase (chloride peroxidase) from Streptomyces aureofaciens (278 aa); etc. Also similar to M. tuberculosis non-heme haloperoxidases and epoxide hydrolases e.g. Rv1938, Rv3617, etc. O06420 No virulence, detoxification, adaptation ML2269 MMAR_0900 MSMEG_1108 Mb0569 NC_000962.3 Mycobrowser_v2 CDS 646298 647962 . + 0 Rv0555 menD Involved in menaquinone biosynthesis (at the first step) [catalytic activity 1: isochorismate + 2-ketoglutarate = 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate + pyruvate + CO(2)] [catalytic activity 2: 2-oxoglutarate = succinate semialdehyde + CO(2)]. Probable bifunctional menaquinone biosynthesis protein MenD : 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (SHCHC synthase) + 2-oxoglutarate decarboxylase (alpha- ketoglutarate decarboxylase) (KDC) Rv0555, (MTCY25D10.34), len: 554 aa. Probable menD, menaquinone biosynthesis protein, including 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase and 2-oxoglutarate decarboxylase activities. Equivalent to NP_302478.1|NC_002677 putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase / 2-oxoglutarate decarboxylase from Mycobacterium leprae (556 aa). Also similar to others e.g. MEND_BACSU|P23970 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase from Bacillus subtilis (548 aa), FASTA scores: opt: 488, E(): 2.3e-21, (34.3% identity in 545 aa overlap); etc. Cofactor: thiamine pyrophosphate. O06421 No intermediary metabolism and respiration ML2270 MMAR_0901 MSMEG_1109 Mb0570 NC_000962.3 Mycobrowser_v2 CDS 647959 648474 . + 0 Rv0556 Rv0556 Unknown Probable conserved transmembrane protein Rv0556, (MTCY25D10.35), len: 171 aa. Probable conserved transmembrane protein, equivalent to NP_302479.1|NC_002677 putative membrane protein from Mycobacterium leprae (175 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06422 No cell wall and cell processes ML2271 MMAR_0902 MSMEG_1110 Mb0571 NC_000962.3 Mycobrowser_v2 CDS 648536 649672 . + 0 Rv0557 mgtA Involved in lipomannan (LM) biosynthesis Mannosyltransferase MgtA Rv0557, (MTCY25D10.36), len: 378 aa. MgtA (previously known as pimB), mannosyltransferase (see citation below), similar to other various transferases e.g. NP_243554.1|NC_002570 alpha-D-mannose-alpha(1-6)phosphatidyl myo-inositol monomannoside transferase from Bacillus halodurans (381 aa); NP_249533.1|NC_002516 probable glycosyl transferase from Pseudomonas aeruginosa (406 aa); NP_419573.1|NC_002696 glycosyl transferase, group 1 family protein, from Caulobacter crescentus (455 aa); etc. Also similar to Q55598 hypothetical 44.9 kDa protein from synechocystis SP (409 aa), FASTA scores: opt: 703, E(): 0, (33.9% identity in 378 aa overlap); GPI3_YEAST|P32363 n-acetylglucosaminyl-phosphatidylinositol biosynthetic protein (452 aa), FASTA scores: opt: 230, E(): 1.1e-07, (23.5% identity in 328 aa overlap). O06423 No lipid metabolism ML2272 MMAR_0903 MSMEG_1113 Mb0572 NC_000962.3 Mycobrowser_v2 CDS 649689 650393 . + 0 Rv0558 menH Involved in menaquinone biosynthesis (at the last step) converts DMKH2 into MKH2 [catalytic activity: S-adenosyl-L-methionine + demethylmenaquinol = S-adenosyl-L-homocysteine + menaquinol]. Probable ubiquinone/menaquinone biosynthesis methyltransferase MenH (2-heptaprenyl-1,4-naphthoquinone methyltransferase) Rv0558, (MTCY25D10.37), len: 234 aa. Probable menH (alternate gene name: menG), ubiquinone/menaquinone biosynthesis methlytransferase (2-heptaprenyl-1,4-naphthoquinone methyltransferase), equivalent to NP_302480.1|NC_002677 putative ubiquinone/menaquinone biosynthesis methyltransferase from Mycobacterium leprae (238 aa). Also highly similar to others e.g. CAB44537.1|AL078618|T34630 from Streptomyces coelicolor (231 aa); UBIE_ECOLI|P27851 from Escherichia coli strain K12 (251 aa), FASTA scores: opt: 421, E(): 1.2e-21, (43.2% identity in 227 aa overlap); GRC2_BACSU|P31113 from Bacillus subtilis (233 aa), FASTA scores: opt: 345, E(): 1.4e-16, (34.6% identity in 231 aa overlap); etc. Belongs to the UbiE family. Note that previously known as ubiE. P0A638 No intermediary metabolism and respiration ML2273 MMAR_0907 MSMEG_1115 Mb0573 NC_000962.3 Mycobrowser_v2 CDS 650407 650745 . - 0 Rv0559c Rv0559c Unknown Possible conserved secreted protein Rv0559c, (MTCY25D10.38c), len: 112 aa. Possible conserved secreted protein, similar to NP_302481.1|NC_002677 putative secreted protein from Mycobacterium leprae (112 aa). Also similar to Y08B_MYCTU|Q11048 hypothetical 11.6 kDa protein FASTA scores: opt: 111, E(): 011, (25.4% identity in 114 aa overlap). Contains possible N-terminal signal sequence. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06425 No cell wall and cell processes ML2274 MMAR_0908 MSMEG_3493 Mb0574c NC_000962.3 Mycobrowser_v2 CDS 842033 842278 . + 0 Rv0750 Rv0750 Function unknown Conserved hypothetical protein Rv0750, (MTV041.24), len: 81 aa. Conserved hypothetical protein, showing almost perfect overlap with C-terminus of Rv0740|MTV041_14 conserved hypothetical protein from Mycobacterium tuberculosis (175 aa), FASTA scores: (93.8% identity in 81 aa overlap). Possible duplication. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53813 No conserved hypotheticals Mb0772 NC_000962.3 Mycobrowser_v2 CDS 652771 653778 . + 0 Rv0562 grcC1 Possibly supplies polyprenyl diphosphate. Probable polyprenyl-diphosphate synthase GrcC1 (polyprenyl pyrophosphate synthetase) Rv0562, (MTCY25D10.41), len: 335 aa. Probable grcC1, polyprenyl diphosphate synthetase, equivalent to NP_302483.1|NC_002677 polyprenyl diphosphate synthase component from Mycobacterium leprae (330 aa). Also similar to others (generally hepta or hexaprenyl) e.g. GRC3_BACSU|P31114 probable heptaprenyl diphosphate syntetase (348 aa), FASTA scores: opt: 599, E(): 4e-31, (33.2% identity in 307 aa overlap); etc. Also highly similar to Mycobacterium tuberculosis proteins Rv0989c|grcC2|NP_215504.1|MTCI237.03c probable polyprenyl-diphosphate synthase (325 aa); Rv3383c, Rv3398c, etc. Contains PS00444 Polyprenyl synthetases signature 2. Belongs to the FPP/GGPP synthetases family. O06428 No intermediary metabolism and respiration ML2277 MMAR_0911 MSMEG_1133 Mb0577 NC_000962.3 Mycobrowser_v2 CDS 653879 654739 . + 0 Rv0563 htpX Possibly involved in adaptation. Hydrolizes specific peptides and/or proteins. Probable protease transmembrane protein heat shock protein HtpX Rv0563, (MTV039.01, MTCY25D10.42), len: 286 aa. (alternative start at position 654006). Probable htpX, protease heat shock protein X (transmembrane protein), equivalent to NP_302484.1|NC_002677 putative peptidase from Mycobacterium leprae (287 aa). Also highly similar to others e.g. CAC08262.1|AL392146 putative peptidase from Streptomyces coelicolor (287 aa); NP_387431.1|NC_003047 putative protease transmembrane protein from Sinorhizobium meliloti (319 aa); NP_105051.1|NC_002678 heat shock protein (htpX) from Mesorhizobium loti (336 aa); NP_248692.1|NC_000909|U67608|MJU67608_8 heat shock protein HtpX, possibly protease (htpX) from Methanococcus jannaschii (284 aa), FASTA scores: opt: 660, E(): 0, (46.5 identity in 245 aa overlap). Continuation of MTCY25D10.42. Belongs to peptidase family M48 (zinc metalloprotease). Cofactor: Zinc. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P65815 No virulence, detoxification, adaptation ML2278 MMAR_0912 MSMEG_1134 Mb0578 NC_000962.3 Mycobrowser_v2 CDS 654924 655949 . - 0 Rv0564c gpdA1 Involved in de novo phospholipid biosynthesis; glycerol-3 phosphate formation [catalytic activity: SN-glycerol 3-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H]. Probable glycerol-3-phosphate dehydrogenase [NAD(P)+] GpdA1 (NAD(P)H-dependent glycerol-3-phosphate dehydrogenase) (NAD(P)H-dependent dihydroxyacetone-phosphate reductase) Rv0564c, (MTV039.02c), len: 341 aa. Possible gpdA1(alternate gene names: gpsA, glyC), glycerol-3-phosphate dehydrogenase [NAD(P)+] dependent, similar to many other glycerol-3-phosphate dehydrogenases e.g. P46919|GPDA_BACSU from Bacillus subtilis (345 aa), FASTA scores: opt: 731, E(): 0, (37.3% identity in 332 aa overlap); etc. Also similar to Rv2982c|gpdA2|MTCY349.05|Z83018|MTCY349_5 from Mycobacterium tuberculosis (334 aa), FASTA scores: opt: 740, E(): 0, (40.4% identity in 322 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family. P64188 No lipid metabolism ML2280 MMAR_0915 MSMEG_1140 Mb0579c NC_000962.3 Mycobrowser_v2 CDS 656010 657470 . - 0 Rv0565c Rv0565c Function unknown; probably involved in cellular metabolism. Probable monooxygenase Rv0565c, (MTV039.03c), len: 486 aa. Probable monoxygenase, highly similar to NP_301173.1|NC_002677 putative monooxygenase from Mycobacterium leprae (494 aa). Also highly similar to others e.g. NP_421371.1|NC_002696 monooxygenase (flavin-binding family) from Caulobacter crescentus (498 aa); C-terminus of NP_051574.1|NC_000958 arylesterase/monoxygenase from Deinococcus radiodurans (833 aa); P12015|CYMO_ACISP cyclohexanone monooxygenase from Acinetobacter sp. (542 aa), FASTA scores: opt: 354, E(): 2.1e-16, (23.7% identity in 435 aa overlap); etc. Also similar to other putative monoxygenases from Mycobacterium tuberculosis e.g. Rv3854c (489 aa), MTCY01A6.14 (489 aa), MTV013_4 (495 aa), MTCY31.20 (495 aa). O53762 No intermediary metabolism and respiration ML2281 MMAR_0917 MSMEG_2038 Mb0580c NC_000962.3 Mycobrowser_v2 RBS 657477 657480 . - 0 Rv0565c Rv0565c Function unknown; probably involved in cellular metabolism. Probable monooxygenase Rv0565c, (MTV039.03c), len: 486 aa. Probable monoxygenase, highly similar to NP_301173.1|NC_002677 putative monooxygenase from Mycobacterium leprae (494 aa). Also highly similar to others e.g. NP_421371.1|NC_002696 monooxygenase (flavin-binding family) from Caulobacter crescentus (498 aa); C-terminus of NP_051574.1|NC_000958 arylesterase/monoxygenase from Deinococcus radiodurans (833 aa); P12015|CYMO_ACISP cyclohexanone monooxygenase from Acinetobacter sp. (542 aa), FASTA scores: opt: 354, E(): 2.1e-16, (23.7% identity in 435 aa overlap); etc. Also similar to other putative monoxygenases from Mycobacterium tuberculosis e.g. Rv3854c (489 aa), MTCY01A6.14 (489 aa), MTV013_4 (495 aa), MTCY31.20 (495 aa). O53762 No intermediary metabolism and respiration ML2281 MMAR_0917 MSMEG_2038 Mb0580c NC_000962.3 Mycobrowser_v2 CDS 657548 658039 . - 0 Rv0566c Rv0566c Function unknown Conserved protein Rv0566c, (MTV039.04c), len: 163 aa. Conserved protein, similar to others e.g. P77482|YAJQ_ECOLI hypothetical 19.0 KDa protein from Escherichia coli (169 aa), FASTA scores: opt: 422, E(): 5.4e-20, (44.1 identity in 161 aa overlap); etc. P67380 No conserved hypotheticals ML2282 MMAR_0920 MSMEG_1165 Mb0581c NC_000962.3 Mycobrowser_v2 RBS 658050 658056 . - 0 Rv0566c Rv0566c Function unknown Conserved protein Rv0566c, (MTV039.04c), len: 163 aa. Conserved protein, similar to others e.g. P77482|YAJQ_ECOLI hypothetical 19.0 KDa protein from Escherichia coli (169 aa), FASTA scores: opt: 422, E(): 5.4e-20, (44.1 identity in 161 aa overlap); etc. P67380 No conserved hypotheticals ML2282 MMAR_0920 MSMEG_1165 Mb0581c NC_000962.3 Mycobrowser_v2 CDS 2546488 2546805 . - 0 Rv2274c mazF8 Unknown Possible toxin MazF8 Rv2274c, (MTCY339.36), len: 105 aa. Possible mazF8, toxin, part of toxin-antitoxin (TA) operon with Rv2274A (See Pandey and Gerdes, 2005). Questionable ORF. Q50689 No virulence, detoxification, adaptation Mb2297c NC_000962.3 Mycobrowser_v2 CDS 667252 668394 . - 0 Rv0574c Rv0574c Function unknown Conserved hypothetical protein Rv0574c, (MTV039.12c), len: 380 aa. Conserved hypothetical protein, showing similarity with other hypothetical proteins and polyglutamate synthases (encapsulation proteins) e.g. AAK64444.1|AF377339_5|AF377339 polyglutamate synthase CapA from Myxococcus xanthus (405 aa); M24150|BACCAPABC_3|CapA polyglutamate synthase (encapsulation protein) from B.anthracis (411 aa), FASTA scores: opt: 261, E(): 4.3e-10, (25.8% identity in 287 aa overlap); etc. O53771 No conserved hypotheticals ML2105 Mb0589c NC_000962.3 Mycobrowser_v2 CDS 659450 660868 . + 0 Rv0568 cyp135B1 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Possible cytochrome P450 135B1 Cyp135B1 Rv0568, (MT0594, MTV039.06), len: 472 aa. Possible cyp135B1, cytochrome P450, similar to putative cytochrome P-450 monoxygenases and other cytochrome P-450 related enzymes e.g. P29980|CPXN_ANASP probable cytochrome P450 from Anabaena sp. strain PCC 7120 (459 aa), FASTA scores: opt: 525, E(): 7.2e-27, (31.9% identity in 417 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. Rv0327c|NP_214841.1|NC_000962|CYP135A1|MT0342|MTCY63.32c putative cytochrome P450 (449 aa), FASTA scores: opt: 1080, E(): 0, (40.5% identity in 444 aa overlap); Rv3685c|NP_218202.1|NC_000962 putative cytochrome P450 (476 aa); Rv0136|NP_214650.1|NC_000962 putative cytochrome P450 (441 aa); etc. Contains cytochrome P450 cysteine heme-iron ligand signature (PS00086). P63715 No intermediary metabolism and respiration MMAR_0938 MSMEG_1213 Mb0583 NC_000962.3 Mycobrowser_v2 RBS 658309 658313 . + 0 Rv0569 Rv0569 Function unknown Conserved protein Rv0569, (MTV039.07), len: 88 aa. Conserved protein. C-terminus highly similar to AAA63065.1|U15184|MLU15184_10 hypothetical protein from Mycobacterium leprae (53 aa), FASTA scores: opt: 140, E(): 0.0046, (64.7% identity in 34 aa overlap). Also similar to T36824|SCI35.11 hypothetical protein from Streptomyces coelicolor (64 aa); and N-terminus of T36956 probable DNA-binding protein from Streptomyces coelicolor (323 aa). Also highly similar to Rv2302|MTCY339.07c|NP_216818.1|NC_000962 conserved hypothetical protein from Mycobacterium tuberculosis (80 aa), FASTA scores: opt: 300, E(): 1.4e-13, (61.8% identity in 76 aa overlap). O53766 No conserved hypotheticals ML2100 Mb0584 NC_000962.3 Mycobrowser_v2 RBS 660988 660992 . + 0 Rv0569 Rv0569 Function unknown Conserved protein Rv0569, (MTV039.07), len: 88 aa. Conserved protein. C-terminus highly similar to AAA63065.1|U15184|MLU15184_10 hypothetical protein from Mycobacterium leprae (53 aa), FASTA scores: opt: 140, E(): 0.0046, (64.7% identity in 34 aa overlap). Also similar to T36824|SCI35.11 hypothetical protein from Streptomyces coelicolor (64 aa); and N-terminus of T36956 probable DNA-binding protein from Streptomyces coelicolor (323 aa). Also highly similar to Rv2302|MTCY339.07c|NP_216818.1|NC_000962 conserved hypothetical protein from Mycobacterium tuberculosis (80 aa), FASTA scores: opt: 300, E(): 1.4e-13, (61.8% identity in 76 aa overlap). O53766 No conserved hypotheticals ML2100 Mb0584 NC_000962.3 Mycobrowser_v2 CDS 661003 661269 . + 0 Rv0569 Rv0569 Function unknown Conserved protein Rv0569, (MTV039.07), len: 88 aa. Conserved protein. C-terminus highly similar to AAA63065.1|U15184|MLU15184_10 hypothetical protein from Mycobacterium leprae (53 aa), FASTA scores: opt: 140, E(): 0.0046, (64.7% identity in 34 aa overlap). Also similar to T36824|SCI35.11 hypothetical protein from Streptomyces coelicolor (64 aa); and N-terminus of T36956 probable DNA-binding protein from Streptomyces coelicolor (323 aa). Also highly similar to Rv2302|MTCY339.07c|NP_216818.1|NC_000962 conserved hypothetical protein from Mycobacterium tuberculosis (80 aa), FASTA scores: opt: 300, E(): 1.4e-13, (61.8% identity in 76 aa overlap). O53766 No conserved hypotheticals ML2100 Mb0584 NC_000962.3 Mycobrowser_v2 CDS 661295 663373 . + 0 Rv0570 nrdZ Involved in the DNA replication pathway (at the first reaction). Provides the precursors necessary for DNA synthesis [catalytic activity: 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O = ribonucleoside diphosphate + reduced thioredoxin]. Probable ribonucleoside-diphosphate reductase (large subunit) NrdZ (ribonucleotide reductase) Rv0570, (MTV039.08), len: 692 aa. Probable nrdZ, ribonucleoside-diphosphate reductase, large subunit, highly similar to others e.g. NP_070492.1|NC_000917|NRD|AE000988_11 ribonucleotide reductase from Archaeoglobus fulgidus (752 aa), FASTA scores: opt: 2001, E(): 0, (52.5% identity in 562 aa overlap) (N-terminus shorter); U73619|TAU73619_1|T37459 ribonucleotide reductase from Thermoplasma acidophilum (857 aa), FASTA scores: opt: 1678, E(): 0, (43.7% identity in 723 aa overlap); etc. Belongs to the ribonucleoside diphosphate reductase large chain family. O53767 No information pathways ML2099 Mb0585 NC_000962.3 Mycobrowser_v2 CDS 679229 681862 . + 0 Rv0584 Rv0584 Unknown Possible conserved exported protein Rv0584, (MTV039.22), len: 877 aa. Possible conserved exported protein, similar to other hypothetical proteins which are not necessarily secreted e.g. CAB61925.1|AL133278 putative secreted protein from Streptomyces coelicolor (772 aa); AAD51075.1|AF175722_1|AF175722 immunoreactive 89kD antigen PG87 from Porphyromonas gingivalis (781 aa), FASTA scores: opt: 637, E(): 2.1e-30, (29.1% identity in 794 aa overlap); etc. Contains PS00699 Nitrogenases component 1 alpha and beta subunits signature 1. Has potential N-terminal signal peptide. Predicted to be an outer membrane protein (See Song et al., 2008). O86365 No cell wall and cell processes MMAR_0952 Mb0599 NC_000962.3 Mycobrowser_v2 CDS 663487 664818 . - 0 Rv0571c Rv0571c Function unknown Conserved protein Rv0571c, (MTV039.09c), len: 443 aa. Conserved protein, highly similar to the products of two adjacent orfs in Mycobacterium leprae: AAA63059.1|U15184|U650S|Q50111 hypothetical protein (258 aa), FASTA scores: opt: 1071, E(): 0, (72.5% identity in 233 aa overlap); and AAA63058.1|U15184|U650T hypothetical protein (86 aa), FASTA scores: opt: 192, E(): 6.4e-06, (70.8% identity in 48 aa overlap). Also similar to others e.g. NP_107072.1|NC_002678 hypothetical protein from Mesorhizobium loti (235 aa); NP_213031.1|NC_000918 hypothetical protein from Aquifex aeolicus (175 aa); etc. And similar to part of hypothetical proteins from Mycobacterium tuberculosis e.g. C-terminus of Rv2143|MTCY270.25c|Z95388|NP_216659.1|NC_000962 (352 aa), FASTA scores: opt: 592, E(): 7e-32, (49.3% identity in 205 aa overlap); N-terminus of Rv2030c|NP_216546.1|NC_000962 (681 aa). O53768 No conserved hypotheticals ML2106 Mb0586c NC_000962.3 Mycobrowser_v2 CDS 665851 667242 . - 0 Rv0573c pncB2 Involved in NAD salvage. Phosphoribosylation of nicotinic acid. [catalytic activity: nicotinate + 5-phosphoribosyl 1-pyrophosphate = nicotinate mononucleotide + diphosphate] Nicotinic acid phosphoribosyltransferase PncB2 Rv0573c, (MTV039.11c), len: 463 aa. PncB2, nicotinic acid phosphoribosyltransferase (See Boshoff et al., 2008). Similar to e.g. NP_213718.1|NC_000918 hypothetical protein from Aquifex aeolicus (426 aa); AL109962|T36953|SCJ1.20 conserved hypothetical protein from Streptomyces coelicolor (438 aa), FASTA scores: opt: 1089, E(): 0, (49.4% identity in 385 aa overlap); P_391053.1|Z99120|BSUB0017_57|NC_000964 protein similar to nicotinate phosphoribosyltransferase from Bacillus subtilis (490 aa), FASTA scores: opt: 955, E():0, (43.5% identity in 356 aa overlap); etc. Also similar to Q10641|Y03F_MYCTU|MTCY130.15c|Rv1330c conserved hypothetical protein from Mycobacterium tuberculosis (509 aa), FASTA scores: opt: 761, E(): 0, (38.4% identity in 437 aa overlap). O53770 No intermediary metabolism and respiration Mb0588c NC_000962.3 Mycobrowser_v2 CDS 668579 669745 . - 0 Rv0575c Rv0575c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0575c, (MTV039.13c), len: 388 aa. Possible oxidoreductase, similar to many diverse oxidoreductases and monooxygenases e.g. AL109974|SCF34_5|T36404 probable monooxygenase from Streptomyces coelicolor (407 aa), FASTA scores: opt: 786, E(): 0, (38.7% identity in 398 aa overlap); P96555|AB000564 salicylate hydroxylase from sphingomonas (395 aa), FASTA scores: opt: 267, E():5e-11, (26.4% identity in 390 aa overlap). Also similar to Rv1260|Z77137|MTCY50.22C from Mycobacterium tuberculosis (372 aa), FASTA scores: opt: 762, E(): 0, (40.9% identity in 345 aa overlap). The transcription of this CDS seems to be activated in macrophages (see citation below). O53772 No intermediary metabolism and respiration ML2287 MMAR_0939 MSMEG_1980 Mb0590c NC_000962.3 Mycobrowser_v2 CDS 669848 671152 . + 0 Rv0576 Rv0576 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (possibly ArsR-family) Rv0576, (MTV039.14), len: 434 aa. Probable transcriptional regulator, ArsR family. N-terminus highly similar to others e.g. NP_102487.1|NC_002678 transcriptional regulator from Mesorhizobium loti (104 aa); NP_242952.1|NC_002570 transcriptional regulator (ArsR family) from Bacillus halodurans (109 aa); etc. C-terminal region (~240-434) shows similarity with D67028_1 from Rhodococcus rhodochrous (112 aa); and Rv0738 from Mycobacterium tuberculosis (182 aa). N-terminus also highly similar to Rv2034 from Mycobacterium tuberculosis (107 aa). Contains helix-turn-helix motif at aa 23-43 (Score 1628, +4.73 SD). O53773 No regulatory proteins Mb0591 NC_000962.3 Mycobrowser_v2 RBS 671156 671160 . + 0 Rv0577 TB27.3 Function unknown. Involved in ability to absorb neutral red stain. Conserved protein TB27.3 Rv0577, (MTV039.15), len: 261 aa. TB27.3, conserved protein. Corresponds to O53774|CF30_MYCTU 27 kDa antigen CFP30B from Mycobacterium tuberculosis culture filtrate (260 aa), FASTA scores: opt: 1781, E(): 0, (100.0% identity in 260 aa overlap). Also similar to several hypothetical proteins and hydroxylases from Steptomyces sp. e.g. T35032 probable hydroxylase from Streptomyces coelicolor (263 aa); Q55078 orfA gene product from Streptomyces sp. (275 aa), FASTA scores: E(): 1.5e-1 9, (38.6% identity in 264 aa overlap); D89734_1|P95754 DNA for SgaA SGAA protein from Streptomyces griseus; and SC9B10_20 from Streptomyces coelicolor (267 aa), FASTA score: (38.9 identity in 252 aa overlap). Also similar to Rv0911|MTCY21C12.05 from Mycobacterium tuberculosis (257 aa), FASTA scores: E(): 1.1e-20, (32.0% identity in 259 aa overlap). P0A5N8 No conserved hypotheticals MMAR_3576 Mb0592 NC_000962.3 Mycobrowser_v2 CDS 671166 671951 . + 0 Rv0577 TB27.3 Function unknown. Involved in ability to absorb neutral red stain. Conserved protein TB27.3 Rv0577, (MTV039.15), len: 261 aa. TB27.3, conserved protein. Corresponds to O53774|CF30_MYCTU 27 kDa antigen CFP30B from Mycobacterium tuberculosis culture filtrate (260 aa), FASTA scores: opt: 1781, E(): 0, (100.0% identity in 260 aa overlap). Also similar to several hypothetical proteins and hydroxylases from Steptomyces sp. e.g. T35032 probable hydroxylase from Streptomyces coelicolor (263 aa); Q55078 orfA gene product from Streptomyces sp. (275 aa), FASTA scores: E(): 1.5e-1 9, (38.6% identity in 264 aa overlap); D89734_1|P95754 DNA for SgaA SGAA protein from Streptomyces griseus; and SC9B10_20 from Streptomyces coelicolor (267 aa), FASTA score: (38.9 identity in 252 aa overlap). Also similar to Rv0911|MTCY21C12.05 from Mycobacterium tuberculosis (257 aa), FASTA scores: E(): 1.1e-20, (32.0% identity in 259 aa overlap). P0A5N8 No conserved hypotheticals MMAR_3576 Mb0592 NC_000962.3 Mycobrowser_v2 CDS 671996 675916 . - 0 Rv0578c PE_PGRS7 Function unknown PE-PGRS family protein PE_PGRS7 Rv0578c, (MTV039.16c), len: 1306 aa. PE_PGRS7, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to many other PGRS proteins e.g. MTCY493.04|Z95844 from Mycobacterium tuberculosis (1329 aa), FASTA scores: opt: 3994, E(): 0, (54.6% identity in 1375 aa overlap). Contains two PS00583 pfkB family of carbohydrate kinases signatures possibly fortuitously. Q6MX28 No PE/PPE MMAR_0943 Mb0593c NC_000962.3 Mycobrowser_v2 RBS 675921 675924 . - 0 Rv0578c PE_PGRS7 Function unknown PE-PGRS family protein PE_PGRS7 Rv0578c, (MTV039.16c), len: 1306 aa. PE_PGRS7, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to many other PGRS proteins e.g. MTCY493.04|Z95844 from Mycobacterium tuberculosis (1329 aa), FASTA scores: opt: 3994, E(): 0, (54.6% identity in 1375 aa overlap). Contains two PS00583 pfkB family of carbohydrate kinases signatures possibly fortuitously. Q6MX28 No PE/PPE MMAR_0943 Mb0593c NC_000962.3 Mycobrowser_v2 RBS 676227 676231 . + 0 Rv0579 Rv0579 Function unknown Conserved hypothetical protein Rv0579, (MTV039.17), len: 252 aa. Conserved hypothetical protein, showing some similarity to others e.g. AE001747_4 hypothetical protein from Thermotoga maritima (247 aa), FASTA scores: opt: 612, E(): 0, (39.6% identity in 235 aa overlap); AE001004_2 hypothetical protein from Archaeoglobus fulgidus (159 aa), FASTA scores: opt: 196, E(): 1e-06, (28.3% identity in 159 aa overlap); etc. O53776 No conserved hypotheticals MMAR_0946 Mb0594 NC_000962.3 Mycobrowser_v2 CDS 676238 676996 . + 0 Rv0579 Rv0579 Function unknown Conserved hypothetical protein Rv0579, (MTV039.17), len: 252 aa. Conserved hypothetical protein, showing some similarity to others e.g. AE001747_4 hypothetical protein from Thermotoga maritima (247 aa), FASTA scores: opt: 612, E(): 0, (39.6% identity in 235 aa overlap); AE001004_2 hypothetical protein from Archaeoglobus fulgidus (159 aa), FASTA scores: opt: 196, E(): 1e-06, (28.3% identity in 159 aa overlap); etc. O53776 No conserved hypotheticals MMAR_0946 Mb0594 NC_000962.3 Mycobrowser_v2 CDS 678389 679075 . - 0 Rv0583c lpqN Unknown Probable conserved lipoprotein LpqN Rv0583c, (MTV039.21c), len: 228 aa. Probable lpqN, conserved lipoprotein, equivalent to AAA90989.1|U20446|MK35|U20446|MKU20446_1 lipoprotein precursor from Mycobacterium kansasii (225 aa), FASTA scores: opt: 945, E(): 0, (62.7% identity in 228 aa overlap); and similar to others from Mycobacteria e.g. Rv0040c and Rv1016c from Mycobacterium tuberculosis. Contains N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O53780 No cell wall and cell processes ML1914 MMAR_0950 MSMEG_5456 Mb0598c NC_000962.3 Mycobrowser_v2 RBS 679082 679085 . - 0 Rv0583c lpqN Unknown Probable conserved lipoprotein LpqN Rv0583c, (MTV039.21c), len: 228 aa. Probable lpqN, conserved lipoprotein, equivalent to AAA90989.1|U20446|MK35|U20446|MKU20446_1 lipoprotein precursor from Mycobacterium kansasii (225 aa), FASTA scores: opt: 945, E(): 0, (62.7% identity in 228 aa overlap); and similar to others from Mycobacteria e.g. Rv0040c and Rv1016c from Mycobacterium tuberculosis. Contains N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O53780 No cell wall and cell processes ML1914 MMAR_0950 MSMEG_5456 Mb0598c NC_000962.3 Mycobrowser_v2 CDS 681885 684272 . - 0 Rv0585c Rv0585c Unknown Probable conserved integral membrane protein Rv0585c, (MTV039.23c, MTCY19H5.37), len: 795 aa. Probable conserved integral membrane protein. C-terminus similar to CAB88984.1|AL353864 putative integral membrane protein from Streptomyces coelicolor (299 aa); and C-terminal region of CAC01311.1|AL390968 putative integral membrane protein from Streptomyces coelicolor (925 aa). Also some similarity with Rv0204 from Mycobacterium tuberculosis. O53781 No cell wall and cell processes MMAR_0953 MSMEG_1199 Mb0600c NC_000962.3 Mycobrowser_v2 CDS 684410 685132 . + 0 Rv0586 mce2R Involved in transcriptional mechanism. Probable transcriptional regulatory protein Mce2R (GntR-family) Rv0586, (MTCY19H5.36c), len: 240 aa. Probable mce2R, transcriptional regulator, GntR family, part of mce2 operon, similar to many e.g. P33233|LLDR_ECOLI putative L-lactate dehydrogenase operon regulatory protein from Escherichia coli (258 aa), FASTA scores: opt: 225, E(): 9.3e-08, (26.7% identity in 232 aa overlap); etc. Also similar to other M. tuberculosis transcriptional regulators GntR proteins e.g. Rv3060c, Rv0792c, etc. Contains PS00043 Bacterial regulatory proteins, gntR family signature and probable helix-turn helix motif from aa 35-56 (Score 1531, +4.40 SD). P67741 No regulatory proteins MSMEG_3527 Mb0601 NC_000962.3 Mycobrowser_v2 CDS 685129 685926 . + 0 Rv0587 yrbE2A Unknown Conserved hypothetical integral membrane protein YrbE2A Rv0587, (MTCY19H5.35c), len: 265 aa. YrbE2A, hypothetical unknown integral membrane protein, part of mce2 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07412|Rv0167|MTCI28.07|yrbE1A (265 aa); O53965|Rv1964|MTV051.02|yrbE3A (265 aa); etc. Also highly similar to conserved hypothetical integral membrane proteins of the yrbEA type, e.g. P45392|YRBE_ECOLI hypothetical 27.9 kDa protein from Escherichia coli (260 aa), FASTA scores: opt: 287, E(): 6.1e-12, (21.5% identity in 256 aa overlap); P45030|YRBE_HAEIN|HI1086 hypothetical protein from Haemophilus influenzae (261 aa), FASTA scores: opt: 311, E(): 1.8e-83, (24.2% identity in 265 aa overlap); NP_302654.1|NC_002677 conserved membrane protein from Mycobacterium leprae (267 aa); etc. O07791 No virulence, detoxification, adaptation MSMEG_0132 Mb0602 NC_000962.3 Mycobrowser_v2 CDS 685928 686815 . + 0 Rv0588 yrbE2B Unknown Conserved hypothetical integral membrane protein YrbE2B Rv0588, (MTCY19H5.34c), len: 295 aa. YrbE2B, hypothetical unknown integral membrane protein, part of mce2 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07413|Rv0168|MTCI28.08|yrbE1B (289 aa); O53966|Rv1965|MTV051.03|yrbE3B (271 aa); etc. Also highly similar to conserved hypothetical integral membrane proteins of the yrbEB type, e.g. P45392|YRBE_ECOLI hypothetical 27.9 kDa protein from Escherichia coli (260 aa), FASTA scores: opt: 232, E(): 8.4e-08, (22.1 % identity in 267 aa overlap); P45030|YRBE_HAEIN|HI1086 hypothetical protein from Haemophilus influenzae (261 aa), FASTA scores: opt: 234, E(): 6.3e-08, (24.2% identity in 215 aa overlap); NP_302655.1|NC_002677 conserved membrane protein from Mycobacterium leprae (289 aa); etc. O07790 No virulence, detoxification, adaptation MSMEG_0133 Mb0603 NC_000962.3 Mycobrowser_v2 CDS 699038 699799 . - 0 Rv0602c tcrA Sensor part of a two component regulatory system. Two component DNA binding transcriptional regulatory protein TcrA Rv0602c, (MTCY19H5.20), len: 253 aa. tcrA, two-component DNA-binding response regulator, highly similar to others e.g. NP_107959.1|NC_002678 two-component response regulator from Mesorhizobium loti (239 aa); etc. Also similar to many other Mycobacterium tuberculosis two-component regulators e.g. Q50806|MTCY10G2.16|Rv1033c response regulator homolog TRCR (TCRV) (257 aa), FASTA score: (47.4 identity in 232 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07776 No regulatory proteins Mb0618c NC_000962.3 Mycobrowser_v2 CDS 686821 688035 . + 0 Rv0589 mce2A Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce2A Rv0589, (MTCY19H5.33c), len: 404 aa. Mce2A; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins P72013|MCE1|Rv0169|MTCI28.09|mce1A (454 aa); O53967|MCE3|Rv1966|MTV051.04|mce3A (425 aa); etc. Also highly similar to others e.g. AAD52105.1|AF113402_1|AF113402 mycobacterial cell entry protein from Mycobacterium bovis BCG (454 aa); NP_302656.1|NC_002677 putative cell invasion protein from Mycobacterium leprae (441 aa); CAC12798.1|AL445327 putative secreted protein from Streptomyces coelicolor (418 aa); etc. Also highly similar, but longer 21 aa, to P72013|CAA50257.1|X70901|MTCI28.08 Mcep protein from Mycobacterium tuberculosis (432 aa), FASTA scores: opt: 1324, E(): 0, (62.6% identity in 436 aa overlap). Contains a possible N-terminal signal or anchor sequence. Predicted to be an outer membrane protein (See Song et al., 2008). Note that previously known as mce2. Q79FY7 No virulence, detoxification, adaptation MMAR_4883 MSMEG_0134,MSMEG_5818,MSMEG_6540 Mb0604 NC_000962.3 Mycobrowser_v2 CDS 688032 688859 . + 0 Rv0590 mce2B Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce2B Rv0590, (MTCY19H5.32c), len: 275 aa. Mce2B; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07414|Rv0170|MTCI28.10|mce1B (346 aa); O53968|Rv1967|MTV051.05|mce3B (342 aa); etc. Also highly similar to others e.g. NP_302657.1|NC_002677 putative secreted protein from Mycobacterium leprae (346 aa); P45391|YRBD_ECOLI hypothetical 19.6 kDa protein from Escherichia coli (183 aa), FASTA scores: opt: 160, E(): 0.00099, (28.3% identity in 166 aa overlap); P45029|YRBD_HAEIN|HI1085 hypothetical protein from Haemophilus influenzae (167 aa), FASTA scores: opt: 135, E():0.035, (25.9% identity in 143 aa overlap); etc. Contains possible N-terminal signal or anchor sequence. Predicted to be an outer membrane protein (See Song et al., 2008). O07788 No virulence, detoxification, adaptation Mb0605 NC_000962.3 Mycobrowser_v2 CDS 688808 689062 . + 0 Rv0590A Rv0590A Unknown, but could be involved in host cell invasion. Mce-family related protein Rv0590A, len: 84 aa. Probable continuation of mce2B|Rv0590. Can find no frameshift to account for this. Possible nucleotide G missing at 688793 as there are 5 in Mycobacterium bovis but only 4 in CDC1551. Strong similarity to C-terminus of other Mce proteins e.g. AL583926|AL583926_38 from Mycobacterium leprae strain tn (346 aa), FASTA scores: E(): 1.2e-20, (67.85% identity in 84 aa overlap). Q8VKI0 No virulence, detoxification, adaptation Mb0605 NC_000962.3 Mycobrowser_v2 RBS 689045 689048 . + 0 Rv0591 mce2C Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce2C Rv0591, (MTCY19H5.31c), len: 481 aa. Mce2C; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07415|R0171|MTCI28.11|mce1C (515 aa); O53969|Rv1968|MTV051.06|mce3C (410 aa); etc. Also highly similar to others e.g. NP_302658.1|NC_002677 putative secreted protein from Mycobacterium leprae (519 aa); CAC12796.1|AL445327 putative secreted protein from Streptomyces coelicolor (351 aa); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and may contain N-terminal signal or anchor sequence. Has highly Pro-rich C-terminus. O07787 No virulence, detoxification, adaptation Mb0606 NC_000962.3 Mycobrowser_v2 CDS 689059 690504 . + 0 Rv0591 mce2C Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce2C Rv0591, (MTCY19H5.31c), len: 481 aa. Mce2C; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07415|R0171|MTCI28.11|mce1C (515 aa); O53969|Rv1968|MTV051.06|mce3C (410 aa); etc. Also highly similar to others e.g. NP_302658.1|NC_002677 putative secreted protein from Mycobacterium leprae (519 aa); CAC12796.1|AL445327 putative secreted protein from Streptomyces coelicolor (351 aa); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and may contain N-terminal signal or anchor sequence. Has highly Pro-rich C-terminus. O07787 No virulence, detoxification, adaptation Mb0606 NC_000962.3 Mycobrowser_v2 RBS 690487 690491 . + 0 Rv0592 mce2D Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce2D Rv0592, (MTCY19H5.30c), len: 508 aa. Mce2D; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07416|Rv0172|MTCI28.12|mce1D (530 aa); O53970|Rv1969|MTV051.07|mce3D (423 aa); etc. Also highly similar to others e.g. NP_302659.1|NC_002677 putative secreted protein from Mycobacterium leprae (531 aa); CAC12795.1|AL445327 putative secreted protein from Streptomyces coelicolor (337 aa); etc. Has highly Pro-rich C-terminus and may contain N-terminal signal or anchor sequence. Predicted to be an outer membrane protein (See Song et al., 2008). O07786 No virulence, detoxification, adaptation MSMEG_0137 Mb0607,Mb0608 NC_000962.3 Mycobrowser_v2 CDS 690501 692027 . + 0 Rv0592 mce2D Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce2D Rv0592, (MTCY19H5.30c), len: 508 aa. Mce2D; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07416|Rv0172|MTCI28.12|mce1D (530 aa); O53970|Rv1969|MTV051.07|mce3D (423 aa); etc. Also highly similar to others e.g. NP_302659.1|NC_002677 putative secreted protein from Mycobacterium leprae (531 aa); CAC12795.1|AL445327 putative secreted protein from Streptomyces coelicolor (337 aa); etc. Has highly Pro-rich C-terminus and may contain N-terminal signal or anchor sequence. Predicted to be an outer membrane protein (See Song et al., 2008). O07786 No virulence, detoxification, adaptation MSMEG_0137 Mb0607,Mb0608 NC_000962.3 Mycobrowser_v2 CDS 692024 693232 . + 0 Rv0593 lprL Unknown, but thought to be involved in host cell invasion. Possible Mce-family lipoprotein LprL (Mce-family lipoprotein Mce2E) Rv0593, (MTCY19H5.29c), len: 402 aa. Possible lprL (alternate gene name: mce2E), lipoprotein which belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07417|LPRK|Rv0173|MTCI28.13|mce1E (390 aa); O53971|LPRM|Rv1970|MTV051.08|mce3E (377 aa); etc. Also highly similar to others e.g. NP_302660.1|NC_002677 putative lipoprotein from Mycobacterium leprae (392 aa); CAC12794.1|AL445327 putative secreted protein from Streptomyces coelicolor (413 aa); etc. Contains possible signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O07785 No cell wall and cell processes MSMEG_0138 Mb0609 NC_000962.3 Mycobrowser_v2 CDS 699856 700167 . + 0 Rv0603 Rv0603 Unknown Possible exported protein Rv0603, (MTCY19H5.19c), len: 103 aa. Possible exported protein with hydrophobic stretch at aa 7-29. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07775 No cell wall and cell processes Mb0619 NC_000962.3 Mycobrowser_v2 RBS 693220 693223 . + 0 Rv0594 mce2F Unknown, but thought involved in host cell invasion. Mce-family protein Mce2F Rv0594, (MTCY19H5.28c), len: 516 aa. Mce2F; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), similar to Mycobacterium tuberculosis proteins O07418|Rv0174|MTCI28.14|mce1F (515 aa); O53972|Rv1971|MTV051.09|mce3F (437 aa); etc. Also highly similar to others e.g. NP_302661.1|NC_002677 putative secreted protein from Mycobacterium leprae (516 aa); AAF74993.1|AF143400_1|AF143400|996A027a protein from Mycobacterium avium (80 aa) (similarity on C-terminus); CAC12793.1|AL445327 putative secreted protein from Streptomyces coelicolor (433 aa); etc. Contains possible N-terminal signal or anchor sequence. Predicted to be an outer membrane protein (See Song et al., 2008). O07784 No virulence, detoxification, adaptation Mb0610 NC_000962.3 Mycobrowser_v2 CDS 693237 694787 . + 0 Rv0594 mce2F Unknown, but thought involved in host cell invasion. Mce-family protein Mce2F Rv0594, (MTCY19H5.28c), len: 516 aa. Mce2F; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), similar to Mycobacterium tuberculosis proteins O07418|Rv0174|MTCI28.14|mce1F (515 aa); O53972|Rv1971|MTV051.09|mce3F (437 aa); etc. Also highly similar to others e.g. NP_302661.1|NC_002677 putative secreted protein from Mycobacterium leprae (516 aa); AAF74993.1|AF143400_1|AF143400|996A027a protein from Mycobacterium avium (80 aa) (similarity on C-terminus); CAC12793.1|AL445327 putative secreted protein from Streptomyces coelicolor (433 aa); etc. Contains possible N-terminal signal or anchor sequence. Predicted to be an outer membrane protein (See Song et al., 2008). O07784 No virulence, detoxification, adaptation Mb0610 NC_000962.3 Mycobrowser_v2 CDS 694839 695231 . - 0 Rv0595c vapC4 Unknown Possible toxin VapC4 Rv0595c, (MTCY19H5.27), len: 130 aa. Possible vapC4, toxin, part of toxin-antitoxin (TA) operon with Rv0596c, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to other conserved hypothetical proteins e.g. Rv0627 (135 aa) and Rv0665 (112 aa) from Mycobacterium tuberculosis; and STBB_PSESM|Q52562 plasmid stability protein from Pseudomonas syringae (139 aa), FASTA scores: opt: 131, E(): 0.0035, (35.2% identity in 88 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07783 No virulence, detoxification, adaptation Mb0611c NC_000962.3 Mycobrowser_v2 CDS 695228 695485 . - 0 Rv0596c vapB4 Unknown Possible antitoxin VapB4 Rv0596c, (MTCY19H5.26), len: 85 aa. Possible vapB4, antitoxin, part of toxin-antitoxin (TA) operon with Rv0595c (See Arcus et al., 2005; Pandey and Gerdes, 2005). Highly similar in part to other M. tuberculosis hypothetical proteins e.g. Rv0626, Rv3181c, Rv3385c, Rv3407, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07782 No virulence, detoxification, adaptation Mb0612c NC_000962.3 Mycobrowser_v2 CDS 695668 696903 . - 0 Rv0597c Rv0597c Function unknown Conserved hypothetical protein Rv0597c, (MTCY19H5.25), len: 411 aa. Conserved hypothetical protein, highly similar to Rv3179 conserved hypothetical protein from Mycobacterium tuberculosis (429 aa). Also similar to AAF76191.1|AF271296_1|AF271296 putative ATP/GTP binding protein from Mycobacterium smegmatis (428 aa); Rv2008c|YW09_MYCTU|Q10849 conserved hypothetical protein from Mycobacterium tuberculosis (441 aa), FASTA scores: opt: 270, E(): 3.6e-11, (30.5% identity in 416 aa overlap) (N-terminus longer). Also similar to other hypothetical proteins e.g. NP_085874.1|NC_002679 hypothetical protein from Mesorhizobium loti (435 aa) (N-terminus longer). Contains PS00017 ATP/GTP-binding site motif A (P-loop). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07781 No conserved hypotheticals Mb0613c NC_000962.3 Mycobrowser_v2 CDS 697564 697800 . - 0 Rv0599c vapB27 Unknown Possible antitoxin VapB27 Rv0599c, (MTCY19H5.23), len: 78 aa. Possible vapB27, antitoxin, part of toxin-antitoxin (TA) operon with Rv0598c, see Arcus et al. 2005. Similar to others e.g. Rv2595|Y0B6_MYCTU|Q50626 conserved hypothetical protein from Mycobacterium tuberculosis (81 aa), FASTA scores: opt: 160, E(): 6.2e-07, (35.8% identity in 81 aa overlap). N-terminus shows stong similarity with N-terminus of NP_104908.1|NC_002678 hypothetical protein from Mesorhizobium loti (89 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07779 No virulence, detoxification, adaptation Mb0615c NC_000962.3 Mycobrowser_v2 CDS 697904 698410 . - 0 Rv0600c Rv0600c Sensor part of a two component regulatory system. Two component sensor kinase [second part] Rv0600c, (MTCY19H5.22), len: 168 aa (probable partial CDS). Two-component sensor kinase (second part), similar to part (C-termini) of many others e.g. Q04943|AFQ2_STRCO sensor protein afsq2 from Streptomyces coelicolor (535 aa), FASTA scores: opt: 347, E(): 1.9e-12, (33.0% identity in 206 aa overlap); etc. Note that sequence was checked and no errors were detected, which would allow this and the upstream ORF to be joined. Start changed since first submission (- 39 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07778 No regulatory proteins Mb0616c NC_000962.3 Mycobrowser_v2 CDS 698524 698994 . - 0 Rv0601c Rv0601c Sensor part of a two component regulatory system. Two component sensor kinase [first part] Rv0601c, (MTCY19H5.21), len: 156 aa (probable partial CDS). Two-component sensor kinase (first part), similar to part (N-termini) of others e.g. Q0375|CUTS_STRLI cuts protein from streptomyces lividans (414 aa), FASTA scores: opt: 230, E(): 3.1e-08, (39.1% identity in 115 aa overlap). Note that the sequence was checked and no errors were detected that would allow this and the downstream ORF to be joined. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07777 No regulatory proteins Mb0617c NC_000962.3 Mycobrowser_v2 RBS 700231 700235 . + 0 Rv0604 lpqO Unknown Probable conserved lipoprotein LpqO Rv0604, (MTCY19H5.18c), len: 316 aa. Probable lpqO, conserved lipoprotein, highly similar to Rv2999|lppY putative lipoprotein from Mycobacterium tuberculosis (321 aa), FASTA scores: opt: 1153, E(): 0, (53.2% identity in 312 aa overlap). Contains probable N-terminal signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07774 No cell wall and cell processes Mb0620 NC_000962.3 Mycobrowser_v2 CDS 700239 701189 . + 0 Rv0604 lpqO Unknown Probable conserved lipoprotein LpqO Rv0604, (MTCY19H5.18c), len: 316 aa. Probable lpqO, conserved lipoprotein, highly similar to Rv2999|lppY putative lipoprotein from Mycobacterium tuberculosis (321 aa), FASTA scores: opt: 1153, E(): 0, (53.2% identity in 312 aa overlap). Contains probable N-terminal signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07774 No cell wall and cell processes Mb0620 NC_000962.3 Mycobrowser_v2 CDS 701406 702014 . + 0 Rv0605 Rv0605 Prevents the cointegration of foreign DNA before integration into the chromosome. Possible resolvase Rv0605, (MTCY19H5.17c), len: 202 aa. Possible resolvase for IS_Y349 element, similar to several Mycobacterial hypothetical proteins and weakly similar to Q52563 resolvase from Pseudomonas syringae (210 aa), FASTA scores: opt: 99, E(): 3.1, (35.7% identity in 98 aa overlap). Contains PS00397 Site-specific recombinases active site and probable helix-turn helix motif from aa 9-30 (Score 1815, +5.37 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07773 No insertion seqs and phages Mb0621 NC_000962.3 Mycobrowser_v2 RBS 710995 710999 . + 0 Rv0617 vapC29 Unknown Possible toxin VapC29. Contains PIN domain. Rv0617, (MTCY19H5.04c), len: 133 aa. Possible vapC29, toxin, part of toxin-antitoxin (TA) operon with Rv0616A, contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. Rv2494, Rv3320c, Rv0749, Rv0277c, Rv2530c, etc. O07760 No virulence, detoxification, adaptation Mb0634 NC_000962.3 Mycobrowser_v2 CDS 711006 711407 . + 0 Rv0617 vapC29 Unknown Possible toxin VapC29. Contains PIN domain. Rv0617, (MTCY19H5.04c), len: 133 aa. Possible vapC29, toxin, part of toxin-antitoxin (TA) operon with Rv0616A, contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. Rv2494, Rv3320c, Rv0749, Rv0277c, Rv2530c, etc. O07760 No virulence, detoxification, adaptation Mb0634 NC_000962.3 Mycobrowser_v2 CDS 702016 702759 . + 0 Rv0606 Rv0606 Thought to be required for the transposition of the insertion element IS_1536. Possible transposase (fragment) Rv0606, (MTCY19H5.16c), len: 247 aa. Possible truncated transposase for IS_1536 element, highly similar to N-terminus of other transposases from Mycobacterium tuberculosis e.g. YX16_MYCTU|Q10809|Rv2885c|MT2953|MTCY274.16c putative transposase from Mycobacterium tuberculosis (460 aa), FASTA scores: opt: 1368, E(): 0, (83.5% identity in 237 aa overlap); Rv2978c, Rv0922, Rv3827c, etc. Also similar to N-terminus of MTV002_57|Rv2792 resolvase from M. tuberculosis (193 aa), FASTA score: (87.4% identity in 238 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07772 No insertion seqs and phages Mb0622 NC_000962.3 Mycobrowser_v2 CDS 702813 703199 . + 0 Rv0607 Rv0607 Unknown Hypothetical protein Rv0607, (MTCY19H5.15c), len: 128 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07771 No conserved hypotheticals Mb0623 NC_000962.3 Mycobrowser_v2 RBS 703232 703235 . + 0 Rv0608 vapB28 Unknown Possible antitoxin VapB28 Rv0608, (MTCY19H5.14c), len: 81 aa. Possible vapB28, antitoxin, part of toxin-antitoxin (TA) operon with Rv0609, see Arcus et al. 2005. Similar to several others e.g. Rv0623|P96913|MTCY20H10.04 (84 aa), FASTA scores: opt: 159, E(): 1.2e-09, (43.0% identity in 86 aa overlap); Rv2760c (89 aa); Rv1740 (70 aa), etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07770 No virulence, detoxification, adaptation Mb0624 NC_000962.3 Mycobrowser_v2 CDS 703244 703489 . + 0 Rv0608 vapB28 Unknown Possible antitoxin VapB28 Rv0608, (MTCY19H5.14c), len: 81 aa. Possible vapB28, antitoxin, part of toxin-antitoxin (TA) operon with Rv0609, see Arcus et al. 2005. Similar to several others e.g. Rv0623|P96913|MTCY20H10.04 (84 aa), FASTA scores: opt: 159, E(): 1.2e-09, (43.0% identity in 86 aa overlap); Rv2760c (89 aa); Rv1740 (70 aa), etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07770 No virulence, detoxification, adaptation Mb0624 NC_000962.3 Mycobrowser_v2 RBS 703473 703476 . + 0 Rv0609 vapC28 Unknown Possible toxin VapC28. Contains PIN domain. Rv0609, (MTCY19H5.13c), len: 133 aa. Possible vapC28, toxin, part of toxin-antitoxin (TA) operon with Rv0608, contains PIN domain, see Arcus et al. 2005. Similar to several Mycobacterium tuberculosis hypothetical proteins e.g. YW37_MYCTU|Q10874|Rv1982c|MT2034|MTCY39.37 conserved hypothetical protein (139 aa), FASTA scores: opt: 262, E(): 8.1e-12, (39.1% identity in 128 aa overlap); MTCY20H10.05|Rv0624|MT0652|MTCY20H10.05 conserved hypothetical protein (131 aa), FASTA score: (42.9% identity in 126 aa overlap), Rv0565c, Rv3854c, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P67238 No virulence, detoxification, adaptation Mb0625 NC_000962.3 Mycobrowser_v2 CDS 703486 703887 . + 0 Rv0609 vapC28 Unknown Possible toxin VapC28. Contains PIN domain. Rv0609, (MTCY19H5.13c), len: 133 aa. Possible vapC28, toxin, part of toxin-antitoxin (TA) operon with Rv0608, contains PIN domain, see Arcus et al. 2005. Similar to several Mycobacterium tuberculosis hypothetical proteins e.g. YW37_MYCTU|Q10874|Rv1982c|MT2034|MTCY39.37 conserved hypothetical protein (139 aa), FASTA scores: opt: 262, E(): 8.1e-12, (39.1% identity in 128 aa overlap); MTCY20H10.05|Rv0624|MT0652|MTCY20H10.05 conserved hypothetical protein (131 aa), FASTA score: (42.9% identity in 126 aa overlap), Rv0565c, Rv3854c, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P67238 No virulence, detoxification, adaptation Mb0625 NC_000962.3 Mycobrowser_v2 CDS 703830 704057 . + 0 Rv0609A Rv0609A Function unknown Conserved hypothetical protein Rv0609A, len: 75 aa. Conserved hypothetical protein, highly similar to part of upstream ORF Rv0612|MTCY19H5.09c conserved hypothetical protein from Mycobacterium tuberculosis (201 aa), FASTA scores: opt: 154, E(): 1.8e-05, (74.3% identity in 35 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q79FY5 No conserved hypotheticals Mb0626 NC_000962.3 Mycobrowser_v2 ncRNA 704187 704247 . + 0 MTB000052 B55 Unknown Putative small regulatory RNA B55, putative small regulatory RNA (See Arnvig and Young, 2009). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 2545332 2545631 . + 0 Rv2271 Rv2271 Unknown Conserved hypothetical protein Rv2271, (MTCY339.39c), len: 99 aa. Conserved hypothetical protein; some similarity to hypothetical protein AAK01340.1|AF265275_3 (AF265275) from uncultured organism Pu8 (104 aa) E= 4e-10, (34% identity in 91 aa overlap) P64967 No conserved hypotheticals Mb2294 NC_000962.3 Mycobrowser_v2 CDS 706324 706929 . + 0 Rv0612 Rv0612 Function unknown Conserved hypothetical protein Rv0612, (MTCY19H5.09c), len: 201 aa. Conserved hypothetical protein, highly similar, but in part, to downstream ORF Rv0609A conserved hypothetical protein from Mycobacterium tuberculosis (75 aa); and showing weak similarity with other hypothetical proteins from Mycobacterium tuberculosis. Note that first start has been taken although this overlaps slightly with the upstream ORF. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07765 No conserved hypotheticals Mb0629 NC_000962.3 Mycobrowser_v2 RBS 828126 828130 . + 0 Rv0736 rslA Regulates negatively SIGL|Rv0735 Anti-sigma factor RslA Rv0736, (MTV041.10), len: 250 aa. RslA, anti-sigma factor (See Dainese et al., 2006). Probable membrane protein, showing weak similarity with AL133469|SCM10_32 putative membrane protein from Streptomyces coelicolor (216 aa), FASTA scores: opt: 180, E(): 0.00018, (34.3% identity in 216 aa overlap). Cleaved by Rip|Rv2869c, in M. tuberculosis Erdman (See Sklar et al., 2010). Q7D9D3 No information pathways MMAR_1074 MSMEG_1487 Mb0757 NC_000962.3 Mycobrowser_v2 CDS 828140 828892 . + 0 Rv0736 rslA Regulates negatively SIGL|Rv0735 Anti-sigma factor RslA Rv0736, (MTV041.10), len: 250 aa. RslA, anti-sigma factor (See Dainese et al., 2006). Probable membrane protein, showing weak similarity with AL133469|SCM10_32 putative membrane protein from Streptomyces coelicolor (216 aa), FASTA scores: opt: 180, E(): 0.00018, (34.3% identity in 216 aa overlap). Cleaved by Rip|Rv2869c, in M. tuberculosis Erdman (See Sklar et al., 2010). Q7D9D3 No information pathways MMAR_1074 MSMEG_1487 Mb0757 NC_000962.3 Mycobrowser_v2 CDS 706948 709515 . - 0 Rv0613c Rv0613c Unknown Unknown protein Rv0613c, (MTCY19H5.08), len: 855 aa. Unknown protein. Contains a very short region with strong similarity to several preprotein translocases e.g. P47847|SECA_LISMO preprotein translocase seca subunit (836 aa), FASTA scores: opt: 138, E(): 0.18, (38.6% identity in 70 aa overlap, and 72.7% identity in 22 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07764 No conserved hypotheticals MSMEG_1285 Mb0630c NC_000962.3 Mycobrowser_v2 RBS 709521 709527 . - 0 Rv0613c Rv0613c Unknown Unknown protein Rv0613c, (MTCY19H5.08), len: 855 aa. Unknown protein. Contains a very short region with strong similarity to several preprotein translocases e.g. P47847|SECA_LISMO preprotein translocase seca subunit (836 aa), FASTA scores: opt: 138, E(): 0.18, (38.6% identity in 70 aa overlap, and 72.7% identity in 22 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07764 No conserved hypotheticals MSMEG_1285 Mb0630c NC_000962.3 Mycobrowser_v2 CDS 709356 710348 . + 0 Rv0614 Rv0614 Function unknown Conserved hypothetical protein Rv0614, (MTCY19H5.07c), len: 330 aa. Conserved hypothetical protein, similar in part to Mycobacterium tuberculosis hypothetical proteins e.g. YY16_MYCTU|Q10685|Rv2077c|MT2137|MTCY49.16c conserved hypothetical protein (323 aa), FASTA scores: opt: 200, E(): 0.00016, (28.3% identity in 269 aa overlap); MTCY9F9_15 FASTA score: (40.3% identity in 144 aa overlap), Rv1949c, Rv2542, etc. Several start sites are possible; first start has been chosen. Note that this ORF overlaps with the upstream ORF. Predicted to be an outer membrane protein (See Song et al., 2008). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07763 No conserved hypotheticals MMAR_0116 Mb0631 NC_000962.3 Mycobrowser_v2 CDS 710584 710850 . - 0 Rv0616c Rv0616c Unknown Hypothetical protein Rv0616c, (MTCY19H5.05), len: 88 aa. Hypothetical unknown protein. O07761 No conserved hypotheticals Mb0633c NC_000962.3 Mycobrowser_v2 CDS 710782 711009 . + 0 Rv0616A vapB29 Unknown Possible antitoxin VapB29 Rv0616A, len: 75 aa. Possible vapB29, antitoxin, part of toxin-antitoxin (TA) operon with Rv0617, see Arcus et al. 2005. Similar to many others in M. tuberculosis e.g. Rv2530A (74 aa) 35.9% identity in 78 aa overlap P0CW34 No virulence, detoxification, adaptation NC_000962.3 Mycobrowser_v2 CDS 711536 712231 . + 0 Rv0618 galTa Involved in galactose metabolism (leloir pathway) [catalytic activity: UTP + alpha-D-galactose 1-phosphate = diphosphate + UDP-galactose]. Probable galactose-1-phosphate uridylyltransferase GalTa [first part] Rv0618, (MTCY19H5.03c), len: 231 aa (probable partial CDS). Probable galTa, first part of galactose-1-phosphate uridylyltransferase, highly similar to N-terminal half of other galT proteins e.g. P13212|GAL7_STRLI galactose-1-phosphate uridylyltransferase from Streptomyces lividans (354 aa), FASTA scores: opt: 296, E(): 1.4e-11, (50.8% identity in 177 aa overlap); etc. Also highly similar to N-terminal half of some UDP glucose--hexose-1-phosphate uridylyltransferases. N-terminal 28 aa similar to MTCY20H11.08|Rv0627|MTCY20H11.08 conserved hypothetical protein from Mycobacterium tuberculosis (135 aa), FASTA score: (71.4% identity in 28 overlap). Cosmid sequence is correct but there may be a frameshift mutation in this region which would allow the two ORFs to be joined. Belongs to the galactose-1-phosphate uridylyltransferase family 1. Note that previously known as galT'. Q79FY4 No intermediary metabolism and respiration Mb0635 NC_000962.3 Mycobrowser_v2 CDS 712174 712719 . + 0 Rv0619 galTb Involved in galactose metabolism (leloir pathway) [catalytic activity: UTP + alpha-D-galactose 1-phosphate = diphosphate + UDP-galactose]. Probable galactose-1-phosphate uridylyltransferase GalTb [second part] Rv0619, (MTCY19H5.02c), len: 181 aa (probable partial CDS). Probable galTb, second part of galactose-1-phosphate uridylyltransferase, highly similar to C-terminal half of other galT proteins e.g. P13212|GAL7_STRLI galactose-1-phosphate uridylyltransferase from Streptomyces lividans (354 aa), FASTA scores: opt: 416, E(): 5.2e-22, (43.0% identity in 186 aa overlap), etc. Cosmid sequence is correct but there may be a frameshift mutation in this region which would allow the two ORFS to be joined. Belongs to the galactose-1-phosphate uridylyltransferase family 1. Note that previously known as 'galT. Q79FY3 No intermediary metabolism and respiration Mb0635 NC_000962.3 Mycobrowser_v2 CDS 732393 732821 . + 0 Rv0636 hadB Involved in fatty acid synthesis type II (fas-II) (3R)-hydroxyacyl-ACP dehydratase subunit HadB Rv0636, (MTCY20H10.17), len: 142 aa. HadB, (3R)-hydroxyacyl-ACP dehydratase subunit, equivalent to NP_302286.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (142 aa). Shows structural similarity to six others in Mycobacterium tuberculosis (see Castell et al (2005) below). Also highly similar to CAB77411.1|AL160431|SCD82.08 hypothetical protein from Streptomyces coelicolor (142 aa); and similar to others e.g. U28943|CELE04F6_3 from Caenorhabditis elegans (cosmid E04) (298 aa), FASTA scores: opt: 167, E(): 0.00064, (31.6 identity in 117 aa overlap). P96927 No intermediary metabolism and respiration ML1909 MMAR_0969 MSMEG_1341 Mb0655 NC_000962.3 Mycobrowser_v2 CDS 712716 713807 . + 0 Rv0620 galK Involved in galactose metabolism (leloir pathway) (at the first reaction) [catalytic activity: ATP + D-galactose = ADP + D-galactose 1-phosphate]. Probable galactokinase GalK (galactose kinase) Rv0620, (MTCY19H5.01c, MTCY20H10.01), len: 363 aa. Probable galK, galactokinase, similar to others e.g. P13227|GAL1_STRLI galactokinase from Streptomyces lividans (397 aa); P06976|GAL1_ECOLI galactokinase from Escherichia coli (381 aa), FASTA scores: opt: 669, E(): 0, (35.9% identity in 365 aa overlap); etc. Contains PS00106 Galactokinase signature and PS00560 Serine carboxypeptidases, histidine active site. Belongs to the GHMP kinase family. GALK subfamily. P96910 No intermediary metabolism and respiration MMAR_0941 MSMEG_3692 Mb0636 NC_000962.3 Mycobrowser_v2 CDS 714202 715266 . + 0 Rv0621 Rv0621 Unknown Possible membrane protein Rv0621, (MTCY20H10.02), len: 354 aa. Possible membrane protein; contains potential membrane spanning regions. Also contains PS00017 ATP/GTP-binding site motif A (P-loop). P96911 No cell wall and cell processes Mb0637 NC_000962.3 Mycobrowser_v2 CDS 716410 716664 . + 0 Rv0623 vapB30 Unknown Possible antitoxin VapB30 Rv0623, (MTCY20H10.04), len: 84 aa. Possible vapB30, antitoxin, part of toxin-antitoxin (TA) operon with Rv0624, see Arcus et al. 2005. Also similar to others in Mycobacterium tuberculosis e.g MTCY28_2|Rv1740|MTCY28.02|MTCY04C12.25 conserved hypothetical protein (70 aa), FASTA score: (73.5% identity in 68 aa overlap); MTCY4C12_25|Rv0608|MTCY19H5.14c conserved hypothetical protein (81 aa), FASTA score: (73.5 identity in 68 aa overlap); etc. P96913 No virulence, detoxification, adaptation MSMEG_1283 Mb0639 NC_000962.3 Mycobrowser_v2 CDS 716664 717059 . + 0 Rv0624 vapC30 Unknown Possible toxin VapC30. Contains PIN domain. Rv0624, (MTCY20H10.05), len: 131 aa. Possible vapC30, toxin, part of toxin-antitoxin (TA) operon with Rv0623, contains PIN domain, see Arcus et al. 2005. Highly similar to others in Mycobacterium tuberculosis e.g. Rv1741, Rv0609, Rv2759c,Rv0565c, Rv3854c, Rv3083, etc. P67240 No virulence, detoxification, adaptation MSMEG_1284 Mb0640 NC_000962.3 Mycobrowser_v2 CDS 717153 717893 . - 0 Rv0625c Rv0625c Unknown Probable conserved transmembrane protein Rv0625c, (MTCY20H10.06c), len: 246 aa. Probable conserved transmembrane protein, showing similarity with others e.g. CAB61866.1|AL133252 putative integral membrane protein from Streptomyces coelicolor (249 aa). Also similar to Rv1491c|MTCY277_13 from Mycobacterium tuberculosis. Contains potential membrane spanning regions. P67115 No cell wall and cell processes MMAR_3541 MSMEG_2840 Mb0641c NC_000962.3 Mycobrowser_v2 CDS 718025 718285 . + 0 Rv0626 vapB5 Possible ribonuclease Possible antitoxin VapB5 Rv0626, (MTCY20H10.07), len: 86 aa. Possible vapB5, antitoxin, part of toxin-antitoxin (TA) operon with Rv0627 (See Arcus et al., 2005; Pandey and Gerdes, 2005)., similar to others in Mycobacterium tuberculosis hypothetical proteins e.g. Rv0596c, Rv3385c, Rv3407,Rv3181c, etc. Cofactor: Mg2+ P96916 No virulence, detoxification, adaptation Mb0642 NC_000962.3 Mycobrowser_v2 CDS 718282 718689 . + 0 Rv0627 vapC5 Unknown Possible toxin VapC5 Rv0627, (MTCY20H11.08), len: 135 aa. Possible vapC5, toxin, part of toxin-antitoxin (TA) operon with Rv0626, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. Rv0595c and Rv0665. P96917 No virulence, detoxification, adaptation Mb0643 NC_000962.3 Mycobrowser_v2 CDS 718761 719912 . - 0 Rv0628c Rv0628c Function unknown Conserved hypothetical protein Rv0628c, (MTCY20H10.09c), len: 383 aa. Conserved hypothetical protein, highly similar to Rv0874c|YZ02_MYCTU|Q10536 conserved hypothetical protein from Mycobacterium tuberculosis (386 aa), FASTA scores: opt: 2082, E(): 0, (81.5% identity in 383 aa overlap). Also some similarity to P72543|SPU62616_1 hypothetical protein from Synechococcus, FASTA scores: E(): 2.8e-28, (36.6 identity in 265 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P64729 No conserved hypotheticals Mb0644c NC_000962.3 Mycobrowser_v2 CDS 746363 747037 . + 0 Rv0649 fabD2 Involved in lipid metabolism; fatty acid biosynthesis [catalytic activity: malonyl-CoA + [acyl-carrier protein] = CoA + malonyl-[acyl-carrier protein]]. Possible malonyl CoA-acyl carrier protein transacylase FabD2 (MCT) Rv0649, (MTCY20H10.30), len: 224 aa. Possible fabD2, malonyl CoA-acyl carrier protein transacylase, similar to mtfabd|FABD_MYCTU|Q10501|Rv2243 malonyl CoA-acyl carrier protein transacylase from Mycobacterium tuberculosis (302 aa), FASTA scores: opt: 133, E(): 0.074, (31.3% identity in 147 aa overlap). Q79FX5 No lipid metabolism Mb0668 NC_000962.3 Mycobrowser_v2 CDS 728583 729278 . - 0 Rv0632c echA3 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Probable enoyl-CoA hydratase EchA3 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv0632c, (MTCY20H10.13c), len: 231 aa. Probable echA3, enoyl-CoA hydratase, almost identical to the MTU88877_1 enoyl-CoA hydratase of Mycobacterium tuberculosis field isolate NTI64719, FASTA score: (92.4% identity in 184 aa overlap). Also similar to others e.g. P24162|ECHH_RHOCA enoyl-CoA hydratase from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (257 aa), FASTA scores: opt: 206, E(): 6.3e-07, (31.5% identity in 232 aa overlap); etc. P96907 No lipid metabolism ML1913 MMAR_0962 MSMEG_1331 Mb0649c NC_000962.3 Mycobrowser_v2 CDS 731930 732406 . + 0 Rv0635 hadA Involved in fatty acid synthesis type II (fas-II) (3R)-hydroxyacyl-ACP dehydratase subunit HadA Rv0635, (MTCY20H10.16), len: 158 aa. HadA, (3R)-hydroxyacyl-ACP dehydratase subunit, equivalent to NP_302287.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (159 aa); and highly similar to YV31_MYCLE|P54879 conserved hypothetical protein from Mycobacterium leprae (166 aa), FASTA scores: opt: 387, E(): 5.9e-21, (43.4% identity in 145 aa overlap). Also similar CAB77410.1|AL160431|SCD82.07 hypothetical protein from Streptomyces coelicolor (150 aa). And highly similar to two hypothetical proteins from Mycobacterium tuberculosis: Rv0504c|YV31_MYCTU|Q11168 (166 aa), FASTA scores: opt: 405, E(): 3.2e-22, (45.0% identity in 140 aa overlap); and Rv0637|MTY20H10_19 (2 ORFs downstream) (166 aa), FASTA score: (48.7% identity in 150 aa overlap). P96926 No intermediary metabolism and respiration ML1910 MMAR_0968 MSMEG_1340 Mb0654 NC_000962.3 Mycobrowser_v2 CDS 720005 721732 . - 0 Rv0629c recD Involved in homologous recombination. Probable exonuclease V (alpha chain) RecD (exodeoxyribonuclease V alpha chain) (exodeoxyribonuclease V polypeptide) Rv0629c, (MTCY20H10.10c), len: 575 aa. Probable recD, exonuclease V, alpha chain (exodeoxyribonuclease V, alpha chain) (see citation below), highly similar to other exonucleases e.g. AF157643_3|AAD46809.1|recD Escherichia coli RecD protein homolog from Mycobacterium smegmatis (554 aa); P04993|EX5A_ECOLI|B2819 exodeoxyribonuclease V 67kd polypeptide (exonuclease V alpha chain) from Escherichia coli strain K12 (608 aa), FASTA scores: opt: 512, E(): 1.9e-24, (36.9% identity in 582 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Consists of three subunits; RECB|Rv0630c, RECC|Rv0631c and RECD. P96919 No information pathways MMAR_0956 MSMEG_1325 Mb0645c NC_000962.3 Mycobrowser_v2 CDS 721729 725013 . - 0 Rv0630c recB Involved in homologous recombination. Probable exonuclease V (beta chain) RecB (exodeoxyribonuclease V beta chain)(exodeoxyribonuclease V polypeptide) (chi-specific endonuclease) Rv0630c, (MTCY20H10.11c), len: 1094 aa. Probable recB, exonuclease V, beta chain (exodeoxyribonuclease V, beta chain) (see citation below), highly similar to other exonucleases e.g. AF157643_2|recB|AAD46808.1 Escherichia coli RecB protein homolog from Mycobacterium smegmatis (1083 aa); P08394|EX5B_ECOLI|RORA|B2820 exodeoxyribonuclease V 135 kDa polypeptide (exonuclease V beta chain) from Escherichia coli strain K12 (1180 aa), FASTA scores: opt: 289, E(): 4.3e-11, (29.5 identity in 1059 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the helicase family, UVRD subfamily. Consists of three subunits; RECB, RECC|Rv0631c and recd|Rv0629c. P96920 No information pathways MMAR_0957 MSMEG_1327 Mb0646c,Mb0647c NC_000962.3 Mycobrowser_v2 CDS 754685 754993 . - 0 Rv0659c mazF2 Function unknown Toxin MazF2 Rv0659c, (MTCI376.17), len: 102 aa. MazF2, toxin, part of toxin-antitoxin (TA) operon with Rv0660c (See Pandey and Gerdes, 2005; Zhu et al., 2006), weakly similar to other Mycobacterium tuberculosis hypothetical proteins e.g. Rv1942c, Rv1495, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06780 No virulence, detoxification, adaptation Mb0678c NC_000962.3 Mycobrowser_v2 CDS 725013 728306 . - 0 Rv0631c recC Involved in homologous recombination. Probable exonuclease V (gamma chain) RecC (exodeoxyribonuclease V gamma chain)(exodeoxyribonuclease V polypeptide) Rv0631c, (MTCY20H10.12c), len: 1097 aa. Probable recC, exonuclease V, gamma chain (exodeoxyribonuclease V, gamma chain) (see Mizrahi & Andersen 1998), highly similar to other exonucleases e.g. AF157643_1|RecC|AAD46807.1 Escherichia coli RecC protein homolog from Mycobacterium smegmatis (1085 aa); P07648|EX5C_ECOLI|B2822 exodeoxyribonuclease V 125 kDa polypeptide (exonuclease V gamma chain) from Escherichia coli strain K12 (1122 aa), FASTA scores: opt: 954, E(): 0, (29.2% identity in 1109 aa overlap); etc. Consists of three subunits; RECB|Rv0630c, RECC and recd|Rv0629c. The transcription of this CDS seems to be activated specifically in host granulomas (see Ramakrishnan et al., 2000). P96921 No information pathways MMAR_0958 MSMEG_1328 Mb0648c NC_000962.3 Mycobrowser_v2 CDS 729327 730166 . - 0 Rv0633c Rv0633c Unknown Possible exported protein Rv0633c, (MTCY20H11.14c), len: 279 aa. Possible exported protein; has hydrophobic stretch at aa 23-41. P96923 No cell wall and cell processes Mb0650c NC_000962.3 Mycobrowser_v2 CDS 730320 731033 . - 0 Rv0634c Rv0634c Thought to be involved in glyoxal pathway. Thiolesterase that catalyses the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid [catalytic activity: (S)-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy acid anion]. Possible glyoxalase II (hydroxyacylglutathione hydrolase) (GLX II) Rv0634c, (MTCY20H10.15c), len: 237 aa. Possible glyoxalase II, equivalent to NP_302290.1|NC_002677 putative glyoxylase II from Mycobacterium leprae (238 aa); and similar to U00011_3|Y0BK_MYCLE|Q49649 hypothetical 23.9 kDa protein from Mycobacterium leprae (218 aa), FASTA scores: opt: 281, E(): 3.9e-12, (31.8% identity in 201 aa overlap). Also similar to other glyoxalases and metallo-beta-lactamase family proteins e.g. NP_386770.1|NC_003047 putative hydroxyacylglutathione hydrolase from Sinorhizobium meliloti (256 aa); etc. Also similar to other putative glyoxylases from Mycobacterium tuberculosis e.g. Rv1637c. Belongs to the glyoxalase II family. Cofactor: binds two zinc ions. P96924 No virulence, detoxification, adaptation ML1912 MMAR_0965 MSMEG_1334 Mb0651c NC_000962.3 Mycobrowser_v2 CDS 731113 731364 . + 0 Rv0634A Rv0634A Unknown Unknown protein Rv0634A, len: 83 aa. Unknown protein. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Q8VKH3 No conserved hypotheticals ML1911A MMAR_0966,MMAR_0967 MSMEG_1339 Mb0652 NC_000962.3 Mycobrowser_v2 tRNA 731494 731566 . + 0 MTB000006 thrT - tRNA-Thr (GGT) thrT, tRNA-Thr, anticodon ggt, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v2 tRNA 731603 731676 . + 0 MTB000007 metT - tRNA-Met (CAT) metT, tRNA-Met, anticodon cat, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 731712 731879 . + 0 Rv0634B rpmG2 Involved in translation mechanism 50S ribosomal protein L33 RpmG2 Rv0634B, len: 55 aa. rpmG2, 50S ribosomal protein L33. Note that Mycobacterium tuberculosis has a second rpmG gene: P96925|R33H_MYCTU|Rv2057c|MTCY63A.03|rpmG1 putative 50S ribosomal protein L33 (55 aa), FASTA scores: opt: 391, E(): 2.9e-25, (100.0% identity in 55 aa overlap). Belongs to the L33P family of ribosomal proteins. P0A5W2 No information pathways ML1911 Mb0653 NC_000962.3 Mycobrowser_v2 CDS 732825 733325 . + 0 Rv0637 hadC Involved in fatty acid synthesis type II (fas-II) (3R)-hydroxyacyl-ACP dehydratase subunit HadC Rv0637, (MTCY20H10.18), len: 166 aa. HadC, (3R)-hydroxyacyl-ACP dehydratase subunit, equivalent to NP_302285.1|NC_002677|YV31_MYCLE|P54879 conserved hypothetical protein from Mycobacterium leprae (166 aa), FASTA scores: opt: 352, E(): 4e-19, (39.2% identity in 148 aa overlap); and highly similar to others from Mycobacterium leprae e.g. NP_302287.1|NC_002677 conserved hypothetical protein (159 aa). Also highly similar to CAB77410.1|AL160431|SCD82.07 hypothetical protein from Streptomyces coelicolor (150 aa); Rv0635|NP_215149.1|NC_000962|MTY20H10_17 conserved hypothetical protein (two ORFs upstream) from Mycobacterium tuberculosis (158 aa), FASTA score: (49.3% identity in 150 aa overlap); and Rv0504c|NP_215018.1|NC_000962|YV31_MYCTU|Q11168 hypothetical protein from Mycobacterium tuberculosis (166 aa), FASTA scores: opt: 380, E(): 3.8e-21, (43.1% identity in 137 aa overlap). P96928 No intermediary metabolism and respiration ML1908 MMAR_0970 MSMEG_1342 Mb0656 NC_000962.3 Mycobrowser_v2 CDS 733737 734222 . + 0 Rv0638 secE1 Essential for protein export. Probable preprotein translocase SecE1 Rv0638, (MTCY20H10.19), len: 161 aa. Probable secE1, preprotein translocase (tail-anchored membrane protein) (see citation below), highly similar at C-terminal half to others e.g. P36690|SECE_STRGR preprotein translocase SECE subunit from Streptomyces griseus (86 aa), FASTA scores: opt: 220, E(): 4.6e-06, (35.4% identity in 96 aa overlap); P16920|SECE_ECOLI preprotein translocase sece subunit from Escherichia coli strains K12 and O157:H7 (127 aa), FASTA scores: opt: 122, E(): 0.34, (37.0% identity in 54 aa overlap); etc. Contains PS01067 Protein secE/sec61-gamma signature. Belongs to the SECE/SEC61-gamma family. Part of the prokaryotic protein translocation apparatus which comprise SECA|Rv3240c, SECD|Rv2587c, SECE, SECF|Rv2586c, SECG|Rv1440 and SECY|Rv0732. Note that previously known as secE. P0A5Z0 No cell wall and cell processes ML1907 MMAR_0971 MSMEG_1344 Mb0657 NC_000962.3 Mycobrowser_v2 CDS 2545737 2546105 . + 0 Rv2272 Rv2272 Unknown Probable conserved transmembrane protein Rv2272, (MTCY339.38c), len: 122 aa. Probable conserved transmembrane protein, similar to YIDH_ECOLI P31445 hypothetical 12.8 kDa protein (115 aa), FASTA scores, opt: 291, E(): 2.9e-14, (45.6% identity in 103 aa overlap), similar to MTCY339.37c, (35.0% identity in 100 aa overlap). P64969 No cell wall and cell processes ML1789 MMAR_3368 MSMEG_1231 Mb2295 NC_000962.3 Mycobrowser_v2 CDS 734254 734970 . + 0 Rv0639 nusG Influences transcription termination and antitermination. Acts as a component of the transcription complex, and interacts with the termination factor rho and RNA polymerase. Probable transcription antitermination protein NusG Rv0639, (MTCY20H10.20), len: 238 aa. Probable nusG, transcription antitermination protein, equivalent to NP_302283.1|NC_002677 transcription antitermination protein nusG from Mycobacterium leprae (228 aa). Also highly similar to others e.g. P36260|NUSG_STRGR from Streptomyces griseus (294 aa), FASTA scores: opt: 845, E(): 0, (55.4% identity in 233 aa overlap); etc. Note that shorter at the N-terminus than other nusG. Contains PS01014 Transcription termination factor nusG signature. Belongs to the NusG family. P65589 No information pathways ML1906 MMAR_0972 MSMEG_1345 Mb0658 NC_000962.3 Mycobrowser_v2 CDS 735022 735450 . + 0 Rv0640 rplK This protein binds directly to 23S ribosomal RNA. 50S ribosomal protein L11 RplK Rv0640, (MTCY20H11.21), len: 142 aa. rplK, 50S ribosomal protein L11, equivalent to NP_302282.1|NC_002677 50S ribosomal protein L11 from Mycobacterium leprae (142 aa). Also highly similar to others e.g. P48954|RL11_STRCO|SCD82.19 50s ribosomal protein L11 from Streptomyces coelicolor (144 aa), FASTA scores: opt: 763, E(): 0, (84.6% identity in 143 aa overlap); etc. Contains PS00359 Ribosomal protein L11 signature. Belongs to the L11P family of ribosomal proteins. P66056 No information pathways ML1905 MMAR_0973 MSMEG_1346 Mb0659 NC_000962.3 Mycobrowser_v2 CDS 735517 736224 . + 0 Rv0641 rplA This protein binds directly to 23S ribosomal RNA and is located in the neighborhood of the site where elongation factor TU is bound to the ribosome. 50S ribosomal protein L1 RplA Rv0641, (MTCY20H10.22), len: 235 aa. rplA, 50S ribosomal protein L1, equivalent to NP_302281.1|NC_002677 50S ribosomal protein L1 from Mycobacterium leprae (235 aa). Also highly similar to others e.g. P3625|RL1_STRGR 50s ribosomal protein L1 from Streptomyces griseus (240 aa), FASTA scores: opt: 1081, E(): 0, (72.2% identity in 230 aa overlap); etc. Belongs to the L1P family of ribosomal proteins. P96932 No information pathways ML1904 MMAR_0974 MSMEG_1347 Mb0660 NC_000962.3 Mycobrowser_v2 CDS 741151 742617 . - 0 Rv0647c Rv0647c Function unknown Conserved protein Rv0647c, (MTCY20H10.28c), len: 488 aa. Conserved protein, equivalent to NP_302277.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (448 aa). Also showing similarity to a variety of hypothetical ABC1-like proteins or conserved hypothetical proteins e.g. D90908_28|P73627 ABC1-like protein from Synechocystis (585 aa), FASTA scores: E(): 1.8e-31, (29.1% identity in 474 aa overlap); Q55884 HYPOTHETICAL6 5.0 KD protein (567 aa), FASTA scores: opt: 583, E(): 5.7e-30, (28.1% identity in 416 aa overlap); etc. Also similar to Rv3197 conserved hypothetical protein from Mycobacterium tuberculosis. P96936 No conserved hypotheticals ML1898 MMAR_0982 MSMEG_1353 Mb0666c NC_000962.3 Mycobrowser_v2 CDS 800487 800792 . + 0 Rv0700 rpsJ This protein is involved in the binding of tRNA to the ribosomes, and in the regulation of rRNA biosynthesis (by modulating the efficiency of transcriptional termination). Interacts with NUSB|Rv2533c. 30S ribosomal protein S10 RpsJ (transcription antitermination factor NusE) Rv0700, (MTCY210.19), len: 101 aa. rpsJ (alternate gene name: nusE), 30S ribosomal protein S10 (see Gopal et al., 2001), equivalent to RS10_MYCLE P307653 30S ribosomal protein S10 from Mycobacterium leprae (101 aa), FASTA scores: opt: 645, E(): 0, (97.0% identity in 101 aa overlap). Also highly similar to others e.g. CAB82069.1|AL161803 30S ribosomal protein S10 from Streptomyces coelicolor (102 aa); etc. Contains PS00361 Ribosomal protein S10 signature. Belongs to the S10P family of ribosomal proteins. P0A5X0 No information pathways ML1864 MMAR_1030 MSMEG_1435 Mb0720 NC_000962.3 Mycobrowser_v2 CDS 736298 737203 . - 0 Rv0642c mmaA4 Involved in mycolic acids modification. Catalyzes unusual S-adenosyl-methionine-dependent transformation of a cis-olefin mycolic acid into a secondary alcohol. Catalyzes introduction of a hydroxyl group at the distal position on mycolic acid chains to produce the hydroxyl mycolate. Mycolic acids represent a major constituent of the mycobacterial cell wall complex. Methyl transfer results in formation of a secondary hydroxy group with an adjacent methyl branch; olefinic mycolic acid methyl transferase. Methoxy mycolic acid synthase 4 MmaA4 (methyl mycolic acid synthase 4) (MMA4) (hydroxy mycolic acid synthase) Rv0642c, (MTCY20H10.23c), len: 301 aa. MmaA4, methoxy mycolic acid synthase 4 (methyltransferase) (see citations below). Equivalent to AAC44876|AAC44876.1|cmaA methyl transferase (mycolic acid modification protein) from Mycobacterium bovis BCG strain Pasteur (298 aa); NP_302280.1|NC_002677 methyl mycolic acid synthase 4 from Mycobacterium leprae (298 aa); and highly similar to others from Mycobacteria e.g. downstream ORF P72027|mmaA3|Rv0643c|MTCY20H10.24c putative methoxy mycolic acid synthase 3 from Mycobacterium tuberculosis (293 aa). Phosphorylated in vitro by PknJ|Rv2088 (See Jang et al., 2010). Q79FX8 No lipid metabolism ML1903 Mb0661c NC_000962.3 Mycobrowser_v2 CDS 737268 738149 . - 0 Rv0643c mmaA3 Involved in mycolic acids modification. Catalyzes unusual S-adenosyl-methionine-dependent transformation of a cis-olefin mycolic acid into a secondary alcohol. Catalyzes introduction of a hydroxyl group at the distal position on mycolic acid chains to produce the hydroxyl mycolate. Mycolic acids represent a major constituent of the mycobacterial cell wall complex. Methyl transfer results in formation of a secondary hydroxy group with an adjacent methyl branch; olefinic mycolic acid methyl transferase. Methoxy mycolic acid synthase 3 MmaA3 (methyl mycolic acid synthase 3) (MMA3) (hydroxy mycolic acid synthase) Rv0643c, (MTCY20H10.24c), len: 293 aa. MmaA3, methoxy mycolic acid synthase 3 (methyltransferase) (see citations below). Equivalent to AAC44875|AAC44875.1|cmaB methyl transferase (mycolic acid modification protein) from Mycobacterium bovis BCG strain Pasteur (289 aa); and highly similar to others from Mycobacteria e.g. upstream ORF P72028|mmaA4|Rv0642c|MTCY20H10.23c putative methoxy mycolic acid synthase 4 from Mycobacterium tuberculosis (301 aa). P0CH91 No lipid metabolism ML1902 Mb0662c NC_000962.3 Mycobrowser_v2 CDS 754980 755225 . - 0 Rv0660c mazE2 Function unknown Possible antitoxin MazE2 Rv0660c, (MTCI376.16), len: 81 aa. Possible mazE2, antitoxin, part of toxin-antitoxin (TA) operon with Rv0659c (See Pandey and Gerdes, 2005; Zhu et al., 2006), showing some similarity to AF016485_130 from Halobacterium sp (100 aa), FASTA scores: (32.4% identity in 74 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06779 No virulence, detoxification, adaptation Mb0679c NC_000962.3 Mycobrowser_v2 CDS 738297 739160 . - 0 Rv0644c mmaA2 Involved in mycolic acids modification. Catalyzes unusual S-adenosyl-methionine-dependent transformation of a cis-olefin mycolic acid into a secondary alcohol. Catalyzes introduction of a hydroxyl group at the distal position on mycolic acid chains to produce the hydroxyl mycolate. Mycolic acids represent a major constituent of the mycobacterial cell wall complex. Methyl transfer results in formation of a secondary hydroxy group with an adjacent methyl branch; olefinic mycolic acid methyl transferase. Has also cyclopropane function. Methoxy mycolic acid synthase 2 MmaA2 (methyl mycolic acid synthase 2) (MMA2) (hydroxy mycolic acid synthase) Rv0644c, (MTCY20H10.25c), len: 287 aa. MmaA2, methoxy mycolic acid synthase 2 (methyltransferase) (see citations below). Equivalent to AAC44874|AAC44874.1|cmaC methyl transferase (mycolic acid modification protein) from Mycobacterium bovis BCG strain Pasteur (287 aa); and highly similar to others from Mycobacteria e.g. upstream ORF P72028|mmaA4|Rv0642c|MTCY20H10.23c putative methoxy mycolic acid synthase 4 from Mycobacterium tuberculosis (301 aa). Note that alternative start is at position 739247. Q79FX6 No lipid metabolism ML1901 Mb0663c NC_000962.3 Mycobrowser_v2 CDS 739327 740187 . - 0 Rv0645c mmaA1 Involved in mycolic acids modification. Catalyzes unusual S-adenosyl-methionine-dependent transformation of a cis-olefin mycolic acid into a secondary alcohol. Catalyzes introduction of a hydroxyl group at the distal position on mycolic acid chains to produce the hydroxyl mycolate. Mycolic acids represent a major constituent of the mycobacterial cell wall complex. Methyl transfer results in formation of a secondary hydroxy group with an adjacent methyl branch; olefinic mycolic acid methyl transferase. Methoxy mycolic acid synthase 1 MmaA1 (methyl mycolic acid synthase 1) (MMA1) (hydroxy mycolic acid synthase) Rv0645c, (MTCY20H10.26c), len: 286 aa. MmaA1, methoxy mycolic acid synthase 1 (methyltransferase) (see citations below). Equivalent to NP_302279.1|NC_002677 methyl mycolic acid synthase 1 from Mycobacterium leprae (286 aa); and highly similar to others from Mycobacteria e.g. upstream ORF P72028|mmaA4|Rv0642c|MTCY20H10.23c putative methoxy mycolic acid synthase 4 from Mycobacterium tuberculosis (301 aa). P0C5C3 No lipid metabolism ML1900 MMAR_0980 MSMEG_1203 Mb0664c NC_000962.3 Mycobrowser_v2 RBS 1662370 1662374 . + 0 Rv1473A Rv1473A Possibly involved in transcriptional mechanism Possible transcriptional regulatory protein Rv1473A, len: 63 aa. Possible transcriptional regulator, CDS predicted by GC plot. Similar to SCI8.24c|AL132644_24 putative transcriptional regulator from Streptomyces coelicolor (73 aa), FASTA scores: opt: 210, E(): 1.5e-08, (56.15% identity in 57 aa overlap). Q8VK14 No regulatory proteins MMAR_2280 MSMEG_3141 Mb1509 NC_000962.3 Mycobrowser_v2 CDS 1662381 1662572 . + 0 Rv1473A Rv1473A Possibly involved in transcriptional mechanism Possible transcriptional regulatory protein Rv1473A, len: 63 aa. Possible transcriptional regulator, CDS predicted by GC plot. Similar to SCI8.24c|AL132644_24 putative transcriptional regulator from Streptomyces coelicolor (73 aa), FASTA scores: opt: 210, E(): 1.5e-08, (56.15% identity in 57 aa overlap). Q8VK14 No regulatory proteins MMAR_2280 MSMEG_3141 Mb1509 NC_000962.3 Mycobrowser_v2 CDS 740234 741139 . - 0 Rv0646c lipG Function unknown; lipolytic enzyme involved in cellular metabolism. Probable lipase/esterase LipG Rv0646c, (MTCY20H10.27c), len: 301 aa. Probable lipG, lipase/esterase, equivalent to NP_302278.1|NC_002677 probable hydrolase from Mycobacterium leprae (304 aa). Also highly similar to various hydrolases, especially lipases e.g. AA61351.1|X88895 carboxyl esterase from Acinetobacter calcoaceticus (312 aa), FASTA scores: opt: 867, E(): 0, (50.2% identity in 279 aa overlap); etc. Also similar to transferases e.g. P77026 macrolide 2'-phosphotransferase II from Escherichia coli (279 aa), FASTA scores: E(): 1.3e-14, (32.5% identity in 286 aa overlap). Similar to M. tuberculosis non-heme bromoperoxidases and epoxide hydrolases. P96935 No intermediary metabolism and respiration ML1899 MMAR_0981 MSMEG_1352 Mb0665c NC_000962.3 Mycobrowser_v2 CDS 791658 791846 . + 0 Rv0691A Rv0691A Electron carrier Mycofactocin precursor protein Rv0691A, len: 62 aa. Mycofactocin precursor protein. P0DJC2 No intermediary metabolism and respiration NC_000962.3 Mycobrowser_v2 CDS 742719 746366 . + 0 Rv0648 Rv0648 Alpha-mannosidase activity: hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. Alpha-mannosidase Rv0648, (MTCY20H10.29), len: 1215 aa. Alpha-mannosidase (see citation below), showing some similarity to hypothetical proteins and various sugar hydrolases e.g. SYCSLRA_6|Q55528 hypothetical 1 20.4 kDa protein from Synechocystis (1042 aa), FASTA scores: opt: 260, E(): 3.6e-08, (23.4% identity in 602 aa overlap); etc. Contains PS00659 Glycosyl hydrolases family 5 signature. P96937 No intermediary metabolism and respiration MMAR_0985 MSMEG_1361 Mb0667 NC_000962.3 Mycobrowser_v2 CDS 747037 747945 . + 0 Rv0650 Rv0650 Function unknown; probably involved in specific sugar metabolism or regulation. Possible sugar kinase Rv0650, (MTCY20H10.31), len: 302 aa. Possible sugar kinase, highly similar to others e.g. CAB95296.1|AL359779 putative sugar kinase from Streptomyces coelicolor (317 aa); NP_406512.1|NC_003143 putative sugar kinase from Yersinia pestis (290 aa); NP_229269.1|NC_000853 glucokinase from Thermotoga maritima (317 aa); etc.Contains PS01125 ROK family signature. Belongs to the ROK (NAGC/XYLR) family. P96939 No intermediary metabolism and respiration ML1897 MMAR_0987 MSMEG_1363 Mb0669 NC_000962.3 Mycobrowser_v2 CDS 748276 748812 . + 0 Rv0651 rplJ Involved in translation mechanisms. 50S ribosomal protein L10 RplJ Rv0651, (MTCY20H10.32), len: 178 aa. rplJ, 50S ribosomal protein L10, equivalent to NP_302276.1|NC_002677 50S ribosomal protein L10 from Mycobacterium leprae (177 aa). Also highly similar to others e.g. P36257|RL10_STRGR 50s ribosomal protein L10 from Streptomyces griseus (185 aa), FASTA scores: opt: 633, E(): 0, (59.0 % identity in 173 aa overlap); etc. Belongs to the L10P family of ribosomal proteins. P66044 No information pathways ML1896 MMAR_0990 MSMEG_1364 Mb0670 NC_000962.3 Mycobrowser_v2 CDS 809946 810887 . + 0 Rv0713 Rv0713 Unknown Probable conserved transmembrane protein Rv0713, (MTCY210.32), len: 313 aa. Probable conserved transmembrane protein, similar to Rv3435c|MTCY77_7|O06252 from Mycobacterium tuberculosis (284 aa), FASTA scores: opt: 557, E(): 2.1e-29, (35.8% identity in 282 aa overlap); MLCB2492_12|O32991 hypothetical 10.7 kDa protein from Mycobacterium leprae (95 aa). P95061 No cell wall and cell processes ML1852 MMAR_1044 Mb0734 NC_000962.3 Mycobrowser_v2 CDS 748849 749241 . + 0 Rv0652 rplL Involved in translation mechanisms: seems to be the binding site for several of the factors involved in protein synthesis and appears to be essential for accurate translation. 50S ribosomal protein L7/L12 RplL (SA1) Rv0652, (MTCY20H10.33), len: 130 aa. rplL (alternate gene name: L7|L12), 50S ribosomal protein L7/L12, equivalent to NP_302275.1|NC_002677 50S ribosomal protein L7/L12 from Mycobacterium leprae (130 aa); and P37381|RL7_MYCBO 50s ribosomal protein L7/L12 from Mycobacterium bovis (130 aa). Also highly similar to others e.g. P02396|RL7_STRGR 50S ribosomal protein L7/L12 from Streptomyces griseus (127 aa); etc. Belongs to the L12P family of ribosomal proteins. P0A5V2 No information pathways ML1895 MMAR_0991 MSMEG_1365 Mb0671 NC_000962.3 Mycobrowser_v2 CDS 749234 749929 . - 0 Rv0653c Rv0653c Involved in transcriptional mechanism. Possible transcriptional regulatory protein (probably TetR-family) Rv0653c, (MTCI376.23, MTCY20H10.34c), len: 231 aa. Possible transcriptional regulator, TetR family, similar in N-terminus to others e.g. CAC03642.1|AL391338 putative TetR-family transcriptional regulator from Streptomyces coelicolor (190 aa); Q51597 cam repressor from Pseudomonas putida (186 aa), FASTA scores: opt: 150, E(): 0.00085, (27.8% identity in 97 aa overlap); etc. Also some similarity to Mycobacterium tuberculosis hypothetical transcriptional regulators Rv0681 and Rv1816. Contains probable helix-turn helix motif from aa 27-48 (Score 1156, +3.12 SD). P96941 No regulatory proteins ML1894 Mb0672c NC_000962.3 Mycobrowser_v2 CDS 798833 799444 . + 0 Rv0698 Rv0698 Function unknown Conserved hypothetical protein Rv0698, (MTCY210.15), len: 203 aa. Conserved hypothetical protein, highly similar to C-terminus of Rv3639c|MTY15C10.12 conserved hypothetical protein from Mycobacterium tuberculosis (188 aa), FASTA scores: E(): 2.1e-07, (54.8% identity in 73 aa overlap). P95044 No conserved hypotheticals Mb0718,Mb0717 NC_000962.3 Mycobrowser_v2 RBS 749989 749993 . + 0 Rv0654 Rv0654 Function unknown; probably involved in cellular metabolism. Probable dioxygenase Rv0654, (MTCI376.22), len: 501 aa. Probable dioxygenase, highly similar to others eg AAK06796.1|AF324838_15|AF324838|SimC5 putative dioxygenase (involved in tetraene formation) from Streptomyces antibioticus (456 aa); CAB56138.1| AL117669 putative dioxygenase from Streptomyces coelicolor (503 aa); T51734 neoxanthin cleavage enzyme (9-cis-epoxy-carotenoid dioxygenase) from Arabidopsis thaliana (538 aa); Q53353 lignostilbene-alpha,beta-dioxygenase from Pseudomonas paucimobilis (Sphingomonas paucimobilis), FASTA scores: opt: 280, E(): 2.3e-11, (28.5% identity in 523 aa overlap); etc. Also some similarity with Rv0913c|MTCY21C12.07c possible dioxygenase from Mycobacterium tuberculosis (501 aa), FASTA score: (29.5% identity in 522 aa overlap). O06785 No intermediary metabolism and respiration ML1893 MMAR_0993 Mb0673 NC_000962.3 Mycobrowser_v2 CDS 750000 751505 . + 0 Rv0654 Rv0654 Function unknown; probably involved in cellular metabolism. Probable dioxygenase Rv0654, (MTCI376.22), len: 501 aa. Probable dioxygenase, highly similar to others eg AAK06796.1|AF324838_15|AF324838|SimC5 putative dioxygenase (involved in tetraene formation) from Streptomyces antibioticus (456 aa); CAB56138.1| AL117669 putative dioxygenase from Streptomyces coelicolor (503 aa); T51734 neoxanthin cleavage enzyme (9-cis-epoxy-carotenoid dioxygenase) from Arabidopsis thaliana (538 aa); Q53353 lignostilbene-alpha,beta-dioxygenase from Pseudomonas paucimobilis (Sphingomonas paucimobilis), FASTA scores: opt: 280, E(): 2.3e-11, (28.5% identity in 523 aa overlap); etc. Also some similarity with Rv0913c|MTCY21C12.07c possible dioxygenase from Mycobacterium tuberculosis (501 aa), FASTA score: (29.5% identity in 522 aa overlap). O06785 No intermediary metabolism and respiration ML1893 MMAR_0993 Mb0673 NC_000962.3 Mycobrowser_v2 CDS 751517 752596 . + 0 Rv0655 mkl Thought to be involved in active transport of ribonucleotide across the membrane. Responsible for energy coupling to the transport system. Possible ribonucleotide-transport ATP-binding protein ABC transporter Mkl Rv0655, (MTCI376.21), len: 359 aa. Possible mkl, ribonucleotide-transport ATP-binding protein ABC transporter (see Braibant et al., 2000), equivalent to P30769|MKL_MYCLE|ML1892 possible ribonucleotide transport ATP-binding protein from Mycobacterium leprae (347 aa), FASTA scores: opt: 2021, E(): 0, (92.2% identity in 335 aa overlap). Also highly similar to many e.g. AB92896.1|AL356992 putative ABC-transporter ATP-binding protein from Streptomyces coelicolor (343 aa); NP_253146.1|NC_002516 probable ATP-binding component of ABC transporter from Pseudomonas aeruginosa (269 aa); P45393|YRBF_ECOLI hypothetical ABC transporter ATP-binding protein from Escherichia coli (269 aa), FASTA scores: opt: 644, E(): 3.4e-33, (38.5% identity in 244 aa overlap); etc. Also similar to many other Mycobacterium tuberculosis ABC transporters e.g. P71747|CYSA|Rv2397c|MTCY253.24 (351 aa), FASTA score: (33.6% identity in 241 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). P63357 No cell wall and cell processes ML1892 MMAR_0994 MSMEG_1366 Mb0674 NC_000962.3 Mycobrowser_v2 CDS 752984 753367 . - 0 Rv0656c vapC6 Unknown Possible toxin VapC6 Rv0656c, (MTCI376.20), len: 127 aa. Possible vapC6, toxin, part of toxin-antitoxin (TA) operon with Rv0657c, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to other proteins from Mycobacterium tuberculosis e.g. Rv2757c, Rv2546, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06783 No virulence, detoxification, adaptation Mb0675c NC_000962.3 Mycobrowser_v2 CDS 881075 881464 . - 0 Rv0786c Rv0786c Function unknown Conserved protein Rv0786c, (MTCY369.30c), len: 129 aa. Conserved protein, similar to three other hypothetical proteins from Streptomyces coelicolor e.g. SC7H1.08c|T35703 hypothetical protein (202 aa), FASTA scores: opt: 241, E(): 5.1e-10, (41.0% identity in 105 aa overlap); SC3A7.08|T29426 (211 aa). P71839 No conserved hypotheticals ML2221 MSMEG_5834 Mb0809c NC_000962.3 Mycobrowser_v2 CDS 753462 753617 . - 0 Rv0657c vapB6 Unknown Possible antitoxin VapB6 Rv0657c, (MTCI376.19), len: 51 aa. Possible vapB6, antitoxin, part of toxin-antitoxin (TA) operon with Rv0656c (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similarity with others from Mycobacterium tuberculosis e.g. Rv2009|MT2064.1|MTCY39.08c|YW08_MYCTU|Q10848 (80 aa), FASTA scores: opt: 107, E(): 0.0038, (45.8% identity in 48 aa overlap), Rv2871, Rv1560, etc. Also some similarity with AL020958|SC4H8_7 from Streptomyces coelicolor (66 aa), FASTA score: (41.0% identity in 39 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06782 No virulence, detoxification, adaptation Mb0676c NC_000962.3 Mycobrowser_v2 RBS 753623 753628 . - 0 Rv0657c vapB6 Unknown Possible antitoxin VapB6 Rv0657c, (MTCI376.19), len: 51 aa. Possible vapB6, antitoxin, part of toxin-antitoxin (TA) operon with Rv0656c (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similarity with others from Mycobacterium tuberculosis e.g. Rv2009|MT2064.1|MTCY39.08c|YW08_MYCTU|Q10848 (80 aa), FASTA scores: opt: 107, E(): 0.0038, (45.8% identity in 48 aa overlap), Rv2871, Rv1560, etc. Also some similarity with AL020958|SC4H8_7 from Streptomyces coelicolor (66 aa), FASTA score: (41.0% identity in 39 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06782 No virulence, detoxification, adaptation Mb0676c NC_000962.3 Mycobrowser_v2 CDS 753693 754409 . - 0 Rv0658c Rv0658c Unknown. Supposedly involved in stationary-phase survival. Probable conserved integral membrane protein Rv0658c, (MTCI376.18), len: 238 aa. Probable conserved integral membrane protein, equivalent to a predicted homologous protein from Mycobacterium smegmatis (see citation below), and showing some similarity with P33774|YPRB_ECOLI hypothetical 24.3 kDa protein from Escherichia coli (217 aa), FASTA scores: opt: 174, E(): 5.3e-05, (25.6% identity in 223 aa overlap). Also similar to Rv1863c and Rv0804 from Mycobacterium tuberculosis. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06781 No cell wall and cell processes MMAR_0147 MSMEG_3474 Mb0677c NC_000962.3 Mycobrowser_v2 RBS 754412 754415 . - 0 Rv0658c Rv0658c Unknown. Supposedly involved in stationary-phase survival. Probable conserved integral membrane protein Rv0658c, (MTCI376.18), len: 238 aa. Probable conserved integral membrane protein, equivalent to a predicted homologous protein from Mycobacterium smegmatis (see citation below), and showing some similarity with P33774|YPRB_ECOLI hypothetical 24.3 kDa protein from Escherichia coli (217 aa), FASTA scores: opt: 174, E(): 5.3e-05, (25.6% identity in 223 aa overlap). Also similar to Rv1863c and Rv0804 from Mycobacterium tuberculosis. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06781 No cell wall and cell processes MMAR_0147 MSMEG_3474 Mb0677c NC_000962.3 Mycobrowser_v2 CDS 755335 755772 . - 0 Rv0661c vapC7 Unknown Possible toxin VapC7 Rv0661c, (MTCI376.15), len: 145 aa. Possible vapC7, toxin, part of toxin-antitoxin (TA) operon with Rv0662c, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. Rv2863|MTV003.09|MTV003_7 (126 aa), FASTA scores: E(): 0.00087, (30.4% identity in 125 aa overlap), Rv0749|MTV041.23 (163 aa); Rv0277c, Rv2530c, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06778 No virulence, detoxification, adaptation Mb0680c NC_000962.3 Mycobrowser_v2 CDS 755769 756023 . - 0 Rv0662c vapB7 Unknown Possible antitoxin VapB7 Rv0662c, (MTCI376.14), len: 84 aa. Possible vapB7, antitoxin, part of toxin-antitoxin (TA) operon with Rv0661c (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similarity with others from Mycobacterium tuberculosis e.g. Rv2871, Rv1241, Rv2550c, etc. Start changed since first submission, now 38 aa shorter. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06777 No virulence, detoxification, adaptation Mb0681c NC_000962.3 Mycobrowser_v2 CDS 791831 792160 . + 0 Rv0692 Rv0692 Function unknown Conserved hypothetical protein Rv0692, (MTCY210.09), len: 109 aa. Conserved hypothetical protein, highly similar to U17129|RSU17129_3|AAC77477.1 unknown protein from Rhodococcus erythropolis (95 aa), FASTA scores: opt: 393, E(): 8.8e-22, (68.2% identity in 88 aa overlap). P95038 No conserved hypotheticals ML1871 MMAR_1020 MSMEG_1422 Mb0711 NC_000962.3 Mycobrowser_v2 RBS 756128 756132 . + 0 Rv0663 atsD Thought to play an important role in the mineralization of sulfates [catalytic activity: a phenol sulfate + H2O = a phenol + sulfate]. Possible arylsulfatase AtsD (aryl-sulfate sulphohydrolase) (arylsulphatase) Rv0663, (MTCI376.13c), len: 787 aa. Possible atsD, arylsulfatase, similar to others e.g. P5169|ARS_PSEAE arylsulfatase from Pseudomonas aeruginosa (532 aa), FASTA scores: opt: 653, E(): 0, (33.1% identity in 544 aa overlap); etc. Also similar to P95059|MTCY210.30|ATSA|Rv0711|MTCY210.30 from Mycobacterium tuberculosis (787 aa), FASTA score: (38.9% identity in 769 aa overlap); and other arylsulfatases from Mycobacterium tuberculosis e.g. Rv3299c|ATSB (970 aa), Rv0711, etc. Contains PS00523 Sulfatases signature 1. Belongs to the sulfatase family. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06776 No intermediary metabolism and respiration ML0795 MMAR_4950 Mb0682 NC_000962.3 Mycobrowser_v2 CDS 756137 758500 . + 0 Rv0663 atsD Thought to play an important role in the mineralization of sulfates [catalytic activity: a phenol sulfate + H2O = a phenol + sulfate]. Possible arylsulfatase AtsD (aryl-sulfate sulphohydrolase) (arylsulphatase) Rv0663, (MTCI376.13c), len: 787 aa. Possible atsD, arylsulfatase, similar to others e.g. P5169|ARS_PSEAE arylsulfatase from Pseudomonas aeruginosa (532 aa), FASTA scores: opt: 653, E(): 0, (33.1% identity in 544 aa overlap); etc. Also similar to P95059|MTCY210.30|ATSA|Rv0711|MTCY210.30 from Mycobacterium tuberculosis (787 aa), FASTA score: (38.9% identity in 769 aa overlap); and other arylsulfatases from Mycobacterium tuberculosis e.g. Rv3299c|ATSB (970 aa), Rv0711, etc. Contains PS00523 Sulfatases signature 1. Belongs to the sulfatase family. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06776 No intermediary metabolism and respiration ML0795 MMAR_4950 Mb0682 NC_000962.3 Mycobrowser_v2 RBS 758519 758523 . + 0 Rv0664 vapB8 Unknown Possible antitoxin VapB8 Rv0664, (MTCI376.12c), len: 90 aa. Possible vapB8, antitoxin, part of toxin-antitoxin (TA) operon with Rv0665 (See Arcus et al., 2005; Pandey and Gerdes, 2005). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06775 No virulence, detoxification, adaptation Mb0683 NC_000962.3 Mycobrowser_v2 CDS 758532 758804 . + 0 Rv0664 vapB8 Unknown Possible antitoxin VapB8 Rv0664, (MTCI376.12c), len: 90 aa. Possible vapB8, antitoxin, part of toxin-antitoxin (TA) operon with Rv0665 (See Arcus et al., 2005; Pandey and Gerdes, 2005). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06775 No virulence, detoxification, adaptation Mb0683 NC_000962.3 Mycobrowser_v2 CDS 758801 759139 . + 0 Rv0665 vapC8 Unknown Possible toxin VapC8 Rv0665, (MTCI376.11c), len: 112 aa. Possible vapC8, toxin, part of toxin-antitoxin (TA) operon with Rv0664, contains PIN domain (See Arcus et al. 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. Rv0627 (135 aa), and Rv0595c. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06774 No virulence, detoxification, adaptation Mb0684 NC_000962.3 Mycobrowser_v2 RBS 759124 759127 . + 0 Rv0666 Rv0666 Unknown Possible membrane protein Rv0666, (MTCI376.10c), len: 57 aa. Possible membrane protein; has hydrophobic stretch at aa 29-47. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06773 No cell wall and cell processes Mb0685 NC_000962.3 Mycobrowser_v2 CDS 759136 759309 . + 0 Rv0666 Rv0666 Unknown Possible membrane protein Rv0666, (MTCI376.10c), len: 57 aa. Possible membrane protein; has hydrophobic stretch at aa 29-47. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06773 No cell wall and cell processes Mb0685 NC_000962.3 Mycobrowser_v2 CDS 799629 799850 . + 0 Rv0699 Rv0699 Unknown Hypothetical protein Rv0699, (MTCY210.17), len: 73 aa. Hypothetical unknown protein. P95046 No conserved hypotheticals Mb0719 NC_000962.3 Mycobrowser_v2 RBS 759796 759800 . + 0 Rv0667 rpoB Catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [catalytic activity: N nucleoside triphosphate = N diphosphate + {RNA}(N)]. DNA-directed RNA polymerase (beta chain) RpoB (transcriptase beta chain) (RNA polymerase beta subunit) Rv0667, (MTCI376.08c), len: 1172 aa. RpoB, DNA-directed RNA polymerase, beta chain (see Miller et al., 1994; Ahmad et al., 2000), equivalent to P30760|RPOB_MYCLE|ML1891 DNA-directed RNA polymerase beta chain from Mycobacterium leprae (1178 aa). Also highly similar to others e.g. AAF60349.1|AF242549_1|AF242549 DNA-dependent RNA polymerase beta subunit from Amycolatopsis mediterranei (1167 aa); CAB77428.1|AL160431 DNA-directed RNA polymerase beta chain from Streptomyces coelicolor (1161 aa); etc. Start site chosen on basis of RBS but alternative start exists at position 14359. Belongs to the RNA polymerase beta chain family. P0A680 No information pathways ML1891 MMAR_0995 MSMEG_1367 Mb0686 NC_000962.3 Mycobrowser_v2 CDS 759807 763325 . + 0 Rv0667 rpoB Catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [catalytic activity: N nucleoside triphosphate = N diphosphate + {RNA}(N)]. DNA-directed RNA polymerase (beta chain) RpoB (transcriptase beta chain) (RNA polymerase beta subunit) Rv0667, (MTCI376.08c), len: 1172 aa. RpoB, DNA-directed RNA polymerase, beta chain (see Miller et al., 1994; Ahmad et al., 2000), equivalent to P30760|RPOB_MYCLE|ML1891 DNA-directed RNA polymerase beta chain from Mycobacterium leprae (1178 aa). Also highly similar to others e.g. AAF60349.1|AF242549_1|AF242549 DNA-dependent RNA polymerase beta subunit from Amycolatopsis mediterranei (1167 aa); CAB77428.1|AL160431 DNA-directed RNA polymerase beta chain from Streptomyces coelicolor (1161 aa); etc. Start site chosen on basis of RBS but alternative start exists at position 14359. Belongs to the RNA polymerase beta chain family. P0A680 No information pathways ML1891 MMAR_0995 MSMEG_1367 Mb0686 NC_000962.3 Mycobrowser_v2 RBS 763358 763365 . + 0 Rv0668 rpoC Catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [catalytic activity: N nucleoside triphosphate = N diphosphate + {RNA}(N)]. DNA-directed RNA polymerase (beta' chain) RpoC (transcriptase beta' chain) (RNA polymerase beta' subunit). Rv0668, (MTCI376.07c), len: 1316 aa. RpoC, DNA-directed RNA polymerase, beta' chain (see Miller et al., 1994), equivalent to P30761|RPOC_MYCLE|ML1890|S31146 DNA-directed RNA polymerase beta' chain from Mycobacterium leprae (1316 aa), FASTA scores: opt: 8295, E(): 0, (95.6% identity in 1316 aa overlap). Also highly similar to others e.g. CAB77429.1|AL160431 DNA-directed RNA polymerase beta' chain (fragment) from Streptomyces coelicolor (1059 aa); P37871|RPOC_BACSU from Bacillus subtilis (1199 aa), FASTA scores: opt: 2367, E(): 0, (52.9 identity in 1317 aa overlap); etc. Belongs to the RNA polymerase beta' chain family. P0A674 No information pathways ML1890 MMAR_0996 MSMEG_1368 Mb0687 NC_000962.3 Mycobrowser_v2 CDS 763370 767320 . + 0 Rv0668 rpoC Catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [catalytic activity: N nucleoside triphosphate = N diphosphate + {RNA}(N)]. DNA-directed RNA polymerase (beta' chain) RpoC (transcriptase beta' chain) (RNA polymerase beta' subunit). Rv0668, (MTCI376.07c), len: 1316 aa. RpoC, DNA-directed RNA polymerase, beta' chain (see Miller et al., 1994), equivalent to P30761|RPOC_MYCLE|ML1890|S31146 DNA-directed RNA polymerase beta' chain from Mycobacterium leprae (1316 aa), FASTA scores: opt: 8295, E(): 0, (95.6% identity in 1316 aa overlap). Also highly similar to others e.g. CAB77429.1|AL160431 DNA-directed RNA polymerase beta' chain (fragment) from Streptomyces coelicolor (1059 aa); P37871|RPOC_BACSU from Bacillus subtilis (1199 aa), FASTA scores: opt: 2367, E(): 0, (52.9 identity in 1317 aa overlap); etc. Belongs to the RNA polymerase beta' chain family. P0A674 No information pathways ML1890 MMAR_0996 MSMEG_1368 Mb0687 NC_000962.3 Mycobrowser_v2 CDS 767684 769597 . - 0 Rv0669c Rv0669c Function unknown; hydrolytic enzyme probably involved in cellular metabolism. Possible hydrolase Rv0669c, (MTCI376.05), len: 637 aa. Possible hydrolase, highly similar to various hydrolases (N-terminus shorter) e.g. BAA88409.1|AB028646 alkaline ceramidase from Pseudomonas aeruginosa (670 aa,) FASTA scores: opt: 1490, E(): 0, (41.2% identity in 651 aa overlap); NP_063946.1|NM_019893 mitochondrial ceramidase from Homo sapiens (761 aa); P_446098.1|NM_053646 N-acylsphingosine amidohydrolase 2 from Rattus norvegicus (761 aa); BAB09641.1|AB016885 neutral ceramidase from Arabidopsis thaliana (705 aa); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). O06769 No intermediary metabolism and respiration MMAR_0997 Mb0688c NC_000962.3 Mycobrowser_v2 CDS 811373 811741 . + 0 Rv0714 rplN This protein binds directly to 23S ribosomal RNA. 50S ribosomal protein L14 RplN Rv0714, (MTCY210.33), len: 122 aa. rplN, 50S ribosomal protein L14, equivalent to O32993|MLCB2492_14|ML1849|RL14_MYCLE 50S ribosomal protein L14 from Mycobacterium leprae (122 aa). Also highly similar to others e.g. CAB82080.1|AL161803 50S ribosomal protein L14 from Streptomyces coelicolor (122 aa); P33100|RL14_MICLU 50s ribosomal protein L14 from Micrococcus luteus (122 aa), FASTA scores: opt: 674, E(): 0, (85.2% identity in 122 aa overlap); etc. Contains PS00049 Ribosomal protein L14 signature. Belongs to the L14P family of ribosomal proteins. P66069 No information pathways ML1849 MMAR_1045 MSMEG_1465 Mb0735 NC_000962.3 Mycobrowser_v2 CDS 2348558 2349292 . - 0 Rv2091c Rv2091c Unknown Probable membrane protein Rv2091c, (MTCY49.31c), len: 244 aa. Probable membrane protein; contains potential transmembrane region. Repetitive ORF. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P64939 No cell wall and cell processes ML1334 MMAR_3077 MSMEG_3884 Mb2118c NC_000962.3 Mycobrowser_v2 CDS 769792 770550 . + 0 Rv0670 end Involved in base excision repair. Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5' ends that are base-free deoxyribose 5-phosphate residues [catalytic activity: endonucleolytic cleavage to 5'-phosphooligonucleotide end-products]. Probable endonuclease IV End (endodeoxyribonuclease IV) (apurinase) Rv0670, (MTCI376.04c), len: 252 aa. Probable end (alternate gene name: nfo), endonuclease IV (apurinase) (see citation below), equivalent to END_MYCLE|P30770|NFO|ML1889 probable endonuclease IV (apurinase) from Mycobacterium leprae (252 aa), FASTA scores: opt: 1463, E(): 0, (85.6% identity in 250 aa overlap). Also similar to others e.g. Q9S2N2|END4_STRCO|NFO|SC6E10.05 probable endonuclease IV from Streptomyces coelicolor (294 aa); etc. Contains PS00729 AP endonucleases family 2 signatures 1 and 2 (PS00729, and PS00730). Belongs to the AP endonucleases family 2. Cofactor: binds 3 zinc ions. The transcription of this CDS seems negatively regulated by the product of mce2R|Rv0586 (See Santangelo et al., 2009). P63535 No information pathways ML1889 MMAR_0999 MSMEG_1383 Mb0689 NC_000962.3 Mycobrowser_v2 CDS 770582 771424 . + 0 Rv0671 lpqP Unknown Possible conserved lipoprotein LpqP Rv0671, (MTCI376.03c), len: 280 aa. Possible lpqP, conserved lipoprotein, similar to U00012|B1308_F2_43|Q49658 from Mycobacterium leprae (302 aa), FASTA scores: opt: 449, E(): 2.4e-22, (37.6% identity in 242 aa overlap). Also highly similar to lpqC|Rv3298c|MTCY71.38c putative lipoprotein from Mycobacterium tuberculosis (304 aa). Also similar to a large variety of proteins including various esterases and poly(3-hydroxyalkanoate) depolymerases, e.g. NP_249234.1|NC_002516 hypothetical protein from Pseudomonas aeruginosa (322 aa); C-terminus of AAD45376.1|AF164516_1|AF164516 cinnamoyl ester hydrolase EstA from Piromyces equi (536 aa); part of P52090|PHA1_PSELE poly(3-hydroxyalkanoate) depolymerase C precursor from Pseudomonas lemoignei (414 aa); CAC10310.1|AL442629 putative secreted protein from Streptomyces coelicolor (348 aa); etc. Has a 17 aa signal sequence and contains appropriately positioned (PS00013) Prokaryotic membrane lipoprotein lipid attachment site. O86367 No cell wall and cell processes MMAR_1000 Mb0690 NC_000962.3 Mycobrowser_v2 CDS 778477 778905 . - 0 Rv0677c mmpS5 Unknown Possible conserved membrane protein MmpS5 Rv0677c, (MTV040.05c), len: 142 aa. Possible mmpS5, conserved membrane protein (see Tekaia et al., 1999), highly similar to other Mycobacterial proteins e.g. P54880|MMS4_MYCLE putative membrane protein from Mycobacterium leprae (154 aa), FASTA scores: opt: 443, E(): 1.4e-23, (47.1% identity in 155 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis. Belongs to the MmpS family. Predicted to be an outer membrane protein (See Song et al., 2008). P65380 No cell wall and cell processes MMAR_1006 MSMEG_0226,MSMEG_3495 Mb0696c NC_000962.3 Mycobrowser_v2 CDS 771484 773112 . + 0 Rv0672 fadE8 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE8 Rv0672, (MTCI376.02c), len: 542 aa. Probable fadE8, acyl-CoA dehydrogenase, highly similar to many e.g. CAC33951.1|AL589708 putative acyl-CoA dehydrogenase from Streptomyces coelicolor (557 aa); P33224|AIDB_ECOLI|B4187 aidb protein (acyl-CoA dehydrogenases family) from Escherichia coli strain K12 (546 aa), FASTA scores: opt: 1369, E(): 0, (44.1% identity in 524 aa overlap); etc. Also similar to several other M. tuberculosis proteins e.g. Rv0154c|MTCI5.28c FASTA score: (26.3% identity in 342 aa overlap); etc. Contains acyl-CoA dehydrogenases signature 2 (PS00073). Belongs to the acyl-CoA dehydrogenases family. O86368 No lipid metabolism ML1888 MMAR_1001 MSMEG_1387 Mb0691 NC_000962.3 Mycobrowser_v2 CDS 773123 774061 . + 0 Rv0673 echA4 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Possible enoyl-CoA hydratase EchA4 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv0673, (MTCI376.01c, MTV040.01), len: 312 aa. Possible echA4, enoyl-CoA hydratase, showing similarity with others e.g. NP_419216.1|NC_002696 enoyl-CoA hydratase/isomerase family protein from Caulobacter crescentus (256 aa); Q52995|ECHH_RHIME probable enoyl-CoA hydratase from Sinorhizobium meliloti (257 aa), FASTA scores: opt: 210, E(): 1.2e-06, (27.9% identity in 280 aa overlap); etc. Also similar to other enoyl-CoA hydratases from Mycobacterium tuberculosis e.g. P95279|MTCY09F9.29|ECHA13|Rv1935c|MTCY09F9.29 enoyl-CoA hydratase (318 aa), FASTA score: (27.1% identity in 280 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). O86369 No lipid metabolism ML1887 MMAR_1002 MSMEG_1388 Mb0692 NC_000962.3 Mycobrowser_v2 CDS 774064 774786 . + 0 Rv0674 Rv0674 Function unknown Conserved hypothetical protein Rv0674, (MTV040.02), len: 240 aa. Conserved hypothetical protein, highly similar to AC13063.1|AL445503 conserved hypothetical protein from Streptomyces coelicolor (268 aa); and similar to NP_438100.1|NC_003078 putative regulator of phenylacetic acid degradation ArsR family protein from Sinorhizobium meliloti (306 aa) and other proteins e.g. AB011837|AB011837_13 hypothetical protein from Bacillus halodurans (298 aa), FASTA scores: opt: 148, E(): 0.0081, (25.1% identity in 235 aa overlap); etc. O53782 No conserved hypotheticals ML1886 MMAR_1003 MSMEG_1389 Mb0693 NC_000962.3 Mycobrowser_v2 CDS 774783 775574 . + 0 Rv0675 echA5 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Probable enoyl-CoA hydratase EchA5 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv0675, (MTV040.03), len: 263 aa. Probable echA5, enoyl-CoA hydratase, similar to several e.g. NP_252116.1|NC_002516 probable enoyl CoA-hydratase/isomerase from Pseudomonas aeruginosa (256 aa); Q20376 protein similar to enoyl-CoA hydratase from Caenorhabditis elegans (258 aa), FASTA scores: opt: 697, E(): 0, (47.3% identity in 245 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. Z92669|MTCY8D5_17 (262 aa), FASTA scores: opt: 493, E(): 3.6e-25, (39.1% identity in 243 aa overlap); etc. Q7D9G0 No lipid metabolism ML1885 MMAR_1004 MSMEG_1390 Mb0694 NC_000962.3 Mycobrowser_v2 CDS 775586 778480 . - 0 Rv0676c mmpL5 Unknown. Thought to be involved in fatty acid transport. Probable conserved transmembrane transport protein MmpL5 Rv0676c, (MTV040.04c), len: 964 aa. Probable mmpL5, conserved transmembrane transport protein (see Tekaia et al., 1999), member of RND superfamily, highly similar to other Mycobacterial proteins e.g. MTV037_14, MTCY98_8, MTCY20G9_34, MTCY4D9_15, MTCY48_8, MTCY19G5_6, MTV005_19, etc. Also similar to other Mycobacterial mmpl proteins e.g. P54881|MML4_MYCLE putative membrane protein MMPL4 from Mycobacterium leprae (959 aa), FASTA scores: opt: 3991, E(): 0, (62.8% identity in 933 aa overlap); etc. Belongs to the MmpL family. O53784 No cell wall and cell processes MMAR_1005 MSMEG_0225,MSMEG_3496 Mb0695c NC_000962.3 Mycobrowser_v2 CDS 780042 780416 . - 0 Rv0680c Rv0680c Unknown Probable conserved transmembrane protein Rv0680c, (MTV040.08c), len: 124 aa. Possible conserved transmembrane protein, showing similarity with C-terminal part of Rv0314c|Z96800|MTCY63.19c conserved hypothetical protein from Mycobacterium tuberculosis (220 aa), FASTA scores: opt: 175, E(): 2.2e-05, (31.4% identity in 102 aa overlap). Also some similarity to upstream ORF Rv0679c|MTV040.07c conserved hypothetical threonine rich protein (124 aa), FASTA score: (35.1% identity in 131 aa overlap). Contains possible membrane spanning regions. O53788 No cell wall and cell processes ML1883 MMAR_1009 MSMEG_1394 Mb0699c NC_000962.3 Mycobrowser_v2 CDS 780721 781311 . + 0 Rv0681 Rv0681 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (possibly TetR-family) Rv0681, (MTV040.09), len: 196 aa. Probable transcription regulator, TetR family, similar to others and especially many tetracycline repressors e.g. T34657 probable transcription regulator from Streptomyces coelicolor (189 aa); AF0278|AF027868_40|NP_389788.1|NC_000964 yobS regulator from Bacillus subtilis (191 aa), FASTA scores: opt: 213, E(): 1.6e-07, (28.8% identity in 153 aa overlap); P09164|TER4_ECOLI tetracycline repressor protein from Escherichia coli (217 aa), FASTA scores: opt: 145, E(): 0.0068, (39.0% identity in 59 aa overlap); etc. Contains helix-turn-helix motif at aa 28-49 (Score 1020, +2.66 SD). O53789 No regulatory proteins ML1882 MMAR_1010 MSMEG_1397 Mb0700 NC_000962.3 Mycobrowser_v2 CDS 781560 781934 . + 0 Rv0682 rpsL Protein S12 is involved in the translation initiation step. 30S ribosomal protein S12 RpsL Rv0682, (MTV040.10), len: 124 aa. rpsL, 30S ribosomal protein S12 (see citations below), equivalent to others from Mycobacteria e.g. P41195|RS12_MYCSM 30S ribosomal protein S12 from Mycobacterium smegmatis (124 aa); P51999|RS12_MYCAV 30S ribosomal protein S12 from Mycobacterium avium (124 aa); etc. Also highly similar to others from other organisms e.g. P97222|RS12_STRCO 30S ribosomal protein S12 from Streptomyces roseosporus, lividans and coelicolor (123 aa); etc. Contains PS00055 Ribosomal protein S12 signature. Belongs to the S12P family of ribosomal proteins. Nucleotide position 781922 in the genome sequence has been corrected, A:G resulting in K121K. P41196 No information pathways ML1880 MMAR_1011 MSMEG_1398 Mb0701 NC_000962.3 Mycobrowser_v2 CDS 781934 782404 . + 0 Rv0683 rpsG Protein S7 binds specifically to part of the 3' end of 16S ribosomal RNA. 30S ribosomal protein S7 RpsG Rv0683, (MTV040.11), len: 156 aa. rpsG, 30S ribosomal protein S7 (see citation below), equivalent to others from Mycobacteria e.g. P41193|RS7_MYCSM 30S ribosomal protein S7 from Mycobacterium smegmatis (156 aa), FASTA scores: opt: 986, E(): 0, (96.2% identity in 156 aa overlap); Q53539|RS7_MYCBO 30S ribosomal protein S7 from Mycobacterium bovis (156 aa); etc. Also highly similar to others e.g. Q9L0K4|RS7_STRCO 30S ribosomal protein S7 from Streptomyces coelicolor (156 aa); etc. Contains PS00052 Ribosomal protein S7 signature. Belongs to the S7P family of ribosomal proteins. P41194 No information pathways ML1879 MMAR_1012 MSMEG_1399 Mb0702 NC_000962.3 Mycobrowser_v2 CDS 782485 784590 . + 0 Rv0684 fusA1 This protein promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. Probable elongation factor G FusA1 (EF-G) Rv0684, (MTV040.12, MTCY210.01), len: 701 aa. Probable fusA1, elongation factor G, equivalent to P30767|EFG_MYCLE|S31150 translation elongation factor EF-G from Mycobacterium leprae (701 aa), FASTA scores: opt: 2521, E(): 0, (88.2% identity in 432 aa overlap). Also highly similar to others e.g. CAB81852.1|AL161691 elongation factor G from Streptomyces coelicolor (708 aa); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS00301 GTP-binding elongation factors signature. Belongs to the GTP-binding elongation factor family, EF-G/EF-2 subfamily. Note that previously known as fusA. P0A556 No information pathways ML1878 MMAR_1013 Mb0703 NC_000962.3 Mycobrowser_v2 CDS 784821 786011 . + 0 Rv0685 tuf This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Probable iron-regulated elongation factor TU Tuf (EF-TU) Rv0685, (MTCY210.02), len: 396 aa. Probable tuf, iron-regulated elongation factor EF-Tu, equivalent to JC2262 translation elongation factor Tu from Mycobacterium leprae (396 aa). Also highly similar to others e.g. P42439|EFTU_CORGL elongation factor TU (EF-TU) from Corynebacterium glutamicum (396 aa); etc. Contains PS00017 ATP/GTP-binding site motif A, and PS00301 GTP-binding elongation factors signature. Belongs to the GTP-binding elongation factor family, EF-TU/EF-1A subfamily. Predicted possible vaccine candidate (See Zvi et al., 2008). P0A558 No information pathways ML1877 MMAR_1014 MSMEG_1401 Mb0704 NC_000962.3 Mycobrowser_v2 CDS 786149 786946 . + 0 Rv0686 Rv0686 Unknown Probable membrane protein Rv0686, (MTCY210.03), len: 265 aa. Probable membrane protein, with hydrophobic N-terminus. P95032 No cell wall and cell processes ML1876 MMAR_1015 MSMEG_1406 Mb0705 NC_000962.3 Mycobrowser_v2 CDS 787099 787926 . + 0 Rv0687 Rv0687 Function unknown; possibly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv0687, (MTCY210.04), len: 275 aa. Probable short-chain dehydrogenase/reductase, highly similar to various dehydrogenases (generally SDR family) e.g. U17129|RSU17129_7 short-chain dehydrogenase from Rhodococcus erythropolis (275 aa), FASTA scores: opt: 1112, E(): 0, (61.2% identity in 268 aa overlap); MMU34072_2 steroid dehydrogenase from Musmus culus (260 aa), FASTA scores: opt: 390, E(): 2.2e-17, (34.1% identity in 267 aa overlap); etc. Also similar to MTV002_16|O33292|Rv2750 dehydrogenase from Mycobacterium tuberculosis (272 aa). Contains PS00061 Short-chain alcohol dehydrogenase family signature. P95033 No intermediary metabolism and respiration ML1875 MMAR_1016 MSMEG_1410 Mb0706 NC_000962.3 Mycobrowser_v2 CDS 787940 789160 . + 0 Rv0688 Rv0688 Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Putative ferredoxin reductase Rv0688, (MTCY210.05), len: 406 aa. Putative ferredoxin reductase, highly similar to others e.g. BAB55881.1|AB054975 ferredoxin reductase from Terrabacter sp. DBF63 (410 aa); CAC04223.1|AL391515 putative ferredoxin reductase from Streptomyces coelicolor (420 aa); PPU24215_8|Q51973 P-cumate dioxygenase ferredoxin reductase subunit from Pseudomonas putida (402 aa), FASTA scores: opt: 738, E(): 0, (38.8% identity in 330 aa overlap); etc. Also similar to Rv0253 and Rv1869c from Mycobacterium tuberculosis. Could belong to the bacterial type ferredoxin family. P95034 No intermediary metabolism and respiration ML1874 MMAR_1017 MSMEG_1416 Mb0707 NC_000962.3 Mycobrowser_v2 CDS 790024 791073 . - 0 Rv0690c Rv0690c Function unknown Conserved hypothetical protein Rv0690c, (MTCY210.07c), len: 349 aa. Conserved hypothetical protein, showing similarity with NP_386956.1|NC_003047 conserved hypothetical protein from Sinorhizobium meliloti (358 aa); NP_356573.1|NC_003063 AGR_L_1570p from Agrobacterium tumefaciens (346 aa); NP_421938.1|NC_002696 conserved hypothetical protein from Caulobacter crescentus (370 aa). P95036 No conserved hypotheticals ML1873 MMAR_1018 Mb0709c NC_000962.3 Mycobrowser_v2 CDS 791070 791666 . - 0 Rv0691c Rv0691c Involved in transcriptional mechanism Probable transcriptional regulatory protein Rv0691c, (MTCY210.08c), len: 198 aa. Probable transcriptional regulator, highly similar to AAC77476.1|U17129 unknown protein from Rhodococcus erythropolis (185 aa); and showing similarity with putative regulatory proteins eg STMTCREP_1|TCMR_STRGA|P39885 tetracenomycin c transcriptional repressor from Streptomyces glaucescens (226 aa), FASTA scores: opt: 178, E(): 8.5e-06, (27.9% identity in 201 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop) and probable helix-turn helix motifs from aa 34-55 (Score 1100, +2.93 SD) and 151-172 (Score 1124, +3.02 SD). P95037 No regulatory proteins ML1872 MMAR_1019 MSMEG_1420 Mb0710c NC_000962.3 Mycobrowser_v2 CDS 792157 793332 . + 0 Rv0693 pqqE Required for coenzyme pyrrolo-quinoline-quinone (PQQ) biosynthesis. Probable coenzyme PQQ synthesis protein E PqqE (coenzyme PQQ synthesis protein III) Rv0693, (MTCY210.10), len: 391 aa. Probable pqqE (alternate gene name: pqqIII), coenzyme PQQ synthesis protein E, similar to others AE001109_9|O30258|PQQE coenzyme PQQ synthesis protein from Archaeoglobus fulgidus (375 aa), FASTA scores: E(): 1.6e-16, (28.1% identity in 377 aa overlap); PQQE_ACICA|P07782 coenzyme pqq synthesis protein e from Acinetobacter calcoaceticus (384 aa), FASTA scores: opt: 302, E(): 1.8e-12, (23.9% identity in 377 aa overlap); etc. Also similar to C-terminus of heme biosynthesis proteins e.g. O28270|AF2009 heme biosynthesis protein (NIRJ-2) from Archaeoglobus fulgidus (468 aa). Note that also highly similar to U17129|RSU17129_4|AAC77478.1 unknown protein from Rhodococcus erythropolis (405 aa), FASTA scores: opt: 1997, E(): 0, (73.3% identity in 390 aa overlap). Could belong to the MoaA / NifB / PqqE family. P95039 No intermediary metabolism and respiration ML1870 MMAR_1021 MSMEG_1423 Mb0712 NC_000962.3 Mycobrowser_v2 CDS 793335 794525 . + 0 Rv0694 lldD1 Involved in respiration; catalyzes conversion of lactate into pyruvate [catalytic activity: (S)-lactate + 2 ferricytochrome C = pyruvate + 2 ferrocytochrome C]. Possible L-lactate dehydrogenase (cytochrome) LldD1 Rv0694, (MTCY210.11), len: 396 aa. Possible lldD1, L-lactate dehydrogenase (cytochrome), similar to NP_302368.1|NC_002677 L-lactate dehydrogenase from Mycobacterium leprae (414 aa). Also similar to others e.g. NP_384560.1|NC_003047 putative L-lactate dehydrogenase (cytochrome) protein from Sinorhizobium meliloti (403 aa); NP_251072.1|NC_002516 L-lactate dehydrogenase from Pseudomonas aeruginosa (383 aa); P33232|LLDD_ECOLI L-lactate dehydrogenase (cytochrome) from Escherichia coli strain K12 (396 aa), FASTA scores: opt: 697, E(): 0, (34.5 identity in 380 aa overlap); etc; and also similar to other oxidoreductases. Note that also highly similar to RSU17129_5|AAC77479.1|U17129 unknown protein from Rhodococcus erythropolis (392 aa), FASTA scores: opt: 2006, E(): 0, (74.1% identity in 386 aa overlap). Also similar to lldD2|Rv1872c|MTCY180.46|MTCY359.01 possible L-lactate dehydrogenase (cytochrome) from Mycobacterium tuberculosis (414 aa). Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenases family. P95040 No intermediary metabolism and respiration ML1869 MMAR_1022 MSMEG_1424 Mb0713 NC_000962.3 Mycobrowser_v2 CDS 794715 795470 . + 0 Rv0695 Rv0695 Function unknown Conserved hypothetical protein Rv0695, (MTCY210.12), len: 251 aa. Conserved hypothetical protein, similar to many creatinine amidohydrolases or hypothetical proteins e.g. NP_443048.1|NC_000911 creatinine amidohydrolase from Synechococcus sp. PCC 6803 (273 aa); NP_466169.1|NC_003210 protein similar to creatinine amidohydrolase from Listeria monocytogenes (249 aa); T35153|SC5A7.04c hypothetical protein from Streptomyces coelicolor (273 aa); etc. Note that highly similar to RSU17129_10|AAC77474.1|U17129 unknown protein from Rhodococcus erythropolis (230 aa), FASTA scores: opt: 693, E(): 0, (55.7% identity in 237 aa overlap). P95041 No conserved hypotheticals ML1868 MMAR_1023 MSMEG_1425 Mb0714 NC_000962.3 Mycobrowser_v2 CDS 808746 809645 . + 0 Rv0712 Rv0712 Function unknown Conserved protein Rv0712, (MTCY210.31), len: 299 aa. Conserved protein, similar to others e.g. NP_106128.1|NC_002678 hypothetical protein from Mesorhizobium loti (372 aa); D90901_33|P72841 hypothetical 48.1 kDa protein from Synechocystis sp (410 aa), FASTA scores: E(): 1.1e-07, (28.8% identity in 299 aa overlap); etc. Slight similarity to carboxykinases. Similar to C-terminal part of Rv3703c conserved hypothetical protein from Mycobacterium tuberculosis (425 aa). P95060 No conserved hypotheticals ML1851 MMAR_1043 MSMEG_1226,MSMEG_1452 Mb0733 NC_000962.3 Mycobrowser_v2 CDS 795519 796931 . + 0 Rv0696 Rv0696 Function unknown; probably involved in cellular metabolism. Probable membrane sugar transferase Rv0696, (MTCY210.13), len: 470 aa. Probable membrane sugar transferase, similar (except in N-terminus) to NP_069157.1|NC_000917 glycosyl transferase from Archaeoglobus fulgidus (324 aa); NP_279985.1|NC_002607 rhamnosyl transferase from Halobacterium sp. NRC-1 (299 aa); NP_059113.1|NM_017417 polypeptide N-acetylgalactosaminyltransferase 8 from (637 aa). Note that also highly similar to P46370|YTH1_RHOER hypothetical 55.3 KDA protein from Rhodococcus erythropolis (513 aa), FASTA scores: opt: 1514, E(): 0, (51.8% identity in 469 aa overlap). P95042 No intermediary metabolism and respiration ML1867 MMAR_1024 MSMEG_1426 Mb0715 NC_000962.3 Mycobrowser_v2 CDS 796933 798372 . + 0 Rv0697 Rv0697 Function unknown; probably involved in cellular metabolism. Probable dehydrogenase Rv0697, (MTCY210.14, unknown), len: 479 aa. Probable dehydrogenase, highly similar to P30772|YTUR_MYCLE hypothetical 24 kDa protein from Mycobacterium leprae (220 aa), FASTA scores: opt: 557, E(): 1.7e-28, (46.2% identity in 223 aa overlap). Also highly similar to P46371|YTH2_RHOER hypothetical 53.0 KDA GMC-type oxidoreductase from Rhodococcus erythropolis (493 aa); and similar to many dehydrogenases e.g. NP_250814.1|NC_002516 probable dehydrogenase from Pseudomonas aeruginosa (545 aa); BAA13145.1|D86622 FAD dependent L-sorbose dehydrogenase from Gluconobacter oxydans (531 aa); etc. Also similar to Rv1279 conserved hypothetical protein from Mycobacterium tuberculosis. P95043 No intermediary metabolism and respiration ML1866 MMAR_1025 MSMEG_1428 Mb0716 NC_000962.3 Mycobrowser_v2 CDS 800809 801462 . + 0 Rv0701 rplC This protein binds directly to 23S ribosomal RNA and may participate in the formation of the peptidyltransferase center of the ribosome. 50S ribosomal protein L3 RplC Rv0701, (MTCY210.20), len: 217 aa. rplC, 50S ribosomal protein L3, equivalent to O06044|RL3_MYCBO 50S ribosomal protein L3 from Mycobacterium bovis BCG (217 aa); and P30762|RL3_MYCLE 50S ribosomal protein L3 from Mycobacterium leprae (217 aa). Also highly similar to others e.g. CAB82070.1|AL161803 50S ribosomal protein L3 from Streptomyces coelicolor (214 aa); P52860|RL3_THETH ribosomal protein l3 from Thermus aquaticus (206 aa), FASTA scores: opt: 717, E(): 0, (55.2% identity in 210 aa overlap); etc. Contains PS00474 Ribosomal protein L3 signature. Belongs to the L3P family of ribosomal proteins. P60442 No information pathways ML1863 MMAR_1031 MSMEG_1436 Mb0721 NC_000962.3 Mycobrowser_v2 CDS 801462 802133 . + 0 Rv0702 rplD This protein binds directly and specifically to 23S rRNA. 50S ribosomal protein L4 RplD Rv0702, (MTCY210.21), len: 223 aa. rplD, 50S ribosomal protein L4, equivalent to O06045|RL4_MYCBO 50S ribosomal protein L4 from Mycobacterium bovis BCG (223 aa); O06114|RL4_MYCSM 50S ribosomal protein L4 from Mycobacterium smegmatis (215 aa); and MLCB2492_3 50S ribosomal protein L4 from Mycobacterium leprae (230 aa). Also highly similar to others e.g. CAB82071.1|AL161803 50S ribosomal protein L4 from Streptomyces coelicolor (219 aa); P28601|RL4_BACST 50s ribosomal protein L4 from Bacillus stearothermophilus (207 aa), FASTA scores: opt: 522, E(): 3.5e-26, (42.4% identity in 198 aa overlap); etc. Belongs to the L4P family of ribosomal proteins. P60729 No information pathways ML1862 MMAR_1032 MSMEG_1437 Mb0722 NC_000962.3 Mycobrowser_v2 CDS 802133 802435 . + 0 Rv0703 rplW Binds to a specific region on the 23S rRNA. 50S ribosomal protein L23 RplW Rv0703, (MTCY21.22), len: 100 aa. rplW, 50S ribosomal protein L23, equivalent to O06046|RL23_MYCBO 50S ribosomal protein L23 from Mycobacterium bovis BCG (100 aa); and MLCB2492_4 50S ribosomal protein L23 from Mycobacterium leprae (100 aa). Also highly similar to others e.g. CAB82072.1|AL161803 50S ribosomal protein L23 from Streptomyces coelicolor (139 aa) (N-terminus longer); P04454|RL23_BACST 50s ribosomal protein L23 from Bacillus stearothermophilus (95 aa), FASTA scores: opt: 275, E(): 1.4e-13, (50.5% identity in 95 aa overlap); etc. Contains PS00050 Ribosomal protein L23 signature. Belongs to the L23P family of ribosomal proteins. P95051 No information pathways ML1861 MMAR_1033 MSMEG_1438 Mb0723 NC_000962.3 Mycobrowser_v2 CDS 802528 803370 . + 0 Rv0704 rplB This protein is a primary 23S rRNA-binding protein. It has peptidyltransferase activity. 50S ribosomal protein L2 RplB Rv0704, (MTCY210.23), len: 280 aa. rplB, 50S ribosomal protein L2, equivalent to O06047|RL2_MYCBO 50S ribosomal protein L2 from Mycobacterium bovis BCG (280 aa); and MLCB2492_5M 50S ribosomal protein L2 from Mycobacterium leprae (280 aa). Also highly similar to others e.g. CAB82073.1|AL161803 50S ribosomal protein L2 from Streptomyces coelicolor (278 aa); P42919|RL2_BACSU 50s ribosomal protein l2 (bl2) from Bacillus subtilis (276 aa), FASTA scores: opt: 1179, E(): 0, (61.1% identity in 275 aa overlap); etc. Contains PS00467 Ribosomal protein L2 signature. Belongs to the L2P family of ribosomal proteins. P95052 No information pathways ML1860 MMAR_1034 MSMEG_1439 Mb0724 NC_000962.3 Mycobrowser_v2 CDS 1051544 1052317 . - 0 Rv0941c Rv0941c Function unknown Conserved hypothetical protein Rv0941c, (MTCY10D7.33), len: 257 aa. Conserved hypothetical protein, showing some similarity with parts of several hypothetical proteins from Streptomyces coelicolor e.g. AL035161|SC9C7_20 (860 aa), FASTA scores: opt: 197, E(): 2.6e-05, (34.2% identity in 114 aa overlap). P71568 No conserved hypotheticals ML0146 MMAR_4567 Mb0966c NC_000962.3 Mycobrowser_v2 CDS 803411 803692 . + 0 Rv0705 rpsS Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA. 30S ribosomal protein S19 RpsS Rv0705, (MTCY210.24), len: 93 aa. rpsS, 30S ribosomal protein S19, equivalent to S36895 ribosomal protein S19 from Mycobacterium bovis (93 aa), FASTA scores: opt: 623, E(): 0, (98.9% identity in 93 aa overlap); and NP_302261.1|NC_002677 30S ribosomal protein S19 from Mycobacterium leprae (93 aa). Also highly similar to others e.g. CAB82074.1|AL161803 30S ribosomal protein S19 from Streptomyces coelicolor (93 aa); etc. Contains PS00323 Ribosomal protein S19 signature. Belongs to the S19P family of ribosomal proteins. P0A5X4 No information pathways ML1859 MMAR_1035 MSMEG_1440 Mb0725 NC_000962.3 Mycobrowser_v2 CDS 803689 804282 . + 0 Rv0706 rplV This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, E.G., L4, L17, and L20. It is important during the early stages of 50S reconstitution. 50S ribosomal protein L22 RplV Rv0706, (MTCY210.25), len: 197 aa. rplV, 50S ribosomal protein L22, equivalent to O06115|RL22_MYCSM 50S ribosomal protein L22 from Mycobacterium smegmatis (153 aa); MBS10OPER_7 50S ribosomal protein L22 from Mycobacterium bovis BCG; and MLCB2492_7 50S ribosomal protein L22 from Mycobacterium leprae (175 aa). Also highly similar to others e.g. CAB82075.1|AL161803 50S ribosomal protein L22 from Streptomyces coelicolor (125 aa); P42060|RL22_BACSU 50s ribosomal protein L22 from Bacillus subtilis (113 aa), FASTA scores: opt: 368, E(): 2.4e-13, (52.8% identity in 108 aa overlap); etc. Contains PS00464 Ribosomal protein L22 signature, and contains repetitive sequence at C-terminus. Belongs to the L22P family of ribosomal proteins. P61181 No information pathways ML1858 MMAR_1036 MSMEG_1441 Mb0726 NC_000962.3 Mycobrowser_v2 CDS 850741 851466 . - 0 Rv0756c Rv0756c Unknown Unknown protein Rv0756c, (MTCY369.01c), len: 241 aa. Unknown protein. P71813 No conserved hypotheticals ML2240 MMAR_4943 MSMEG_5873 Mb0779c NC_000962.3 Mycobrowser_v2 CDS 2249478 2250974 . - 0 Rv2004c Rv2004c Unknown Conserved protein Rv2004c, (MTCY39.13), len: 498 aa. Conserved protein. Contains PS00017 ATP/GTP-binding site motif A. P0A5F9 No conserved hypotheticals MMAR_3476 MSMEG_3942 Mb2027c NC_000962.3 Mycobrowser_v2 CDS 804282 805106 . + 0 Rv0707 rpsC This protein is involved in the binding of initiator met-tRNA. 30S ribosomal protein S3 RpsC Rv0707, (MTCY210.26), len: 274 aa. rpsC, 30S ribosomal protein S3, equivalent to O06048|RS3_MYCBO|MBS10OPER_8 30S ribosomal protein S3 from Mycobacterium bovis BCG (274 aa); and MLCB2492_8 30S ribosomal protein S3 from Mycobacterium leprae (281 aa). Also highly similar to others e.g. CAB82076.1|AL161803 30S ribosomal protein S3 from Streptomyces coelicolor (277 aa); P21465|RS3_BACSU 30s ribosomal protein s3 (bs3) (bs2) from Bacillus subtilis (217 aa), FASTA scores: opt: 794, E(): 0, (52.8% identity in 212 aa overlap); etc. Belongs to the S3P family of ribosomal proteins. P0A5X6 No information pathways ML1857 MMAR_1037 MSMEG_1442 Mb0727 NC_000962.3 Mycobrowser_v2 CDS 805110 805526 . + 0 Rv0708 rplP This protein binds directly to 23S ribosomal RNA and is located at the a site of the peptidyltransferase center. 50S ribosomal protein L16 RplP Rv0708, (MTCY210.27), len: 138 aa. rplP, 50S ribosomal protein L16, equivalent to O06049|RL16_MYCBO|MBS10OPER_9 50S ribosomal protein L16 from Mycobacterium bovis BCG (138 aa); and MLCB2492_9 50S ribosomal protein L16 from Mycobacterium leprae (138 aa). Also highly similar to others e.g. CAB82077.1|AL161803 50S ribosomal protein L16 from Streptomyces coelicolor (139 aa); P14577|RL16_BACSU 50s ribosomal protein l16 from Bacillus subtilis (144 aa), FASTA scores: opt: 600, E(): 0, (63.2% identity in 136 aa overlap); etc. Contains PS00701 Ribosomal protein L16 signature 2. Belongs to the L16P family of ribosomal proteins. P95056 No information pathways ML1856 MMAR_1038 MSMEG_1443 Mb0728 NC_000962.3 Mycobrowser_v2 CDS 1052360 1052638 . + 0 Rv0942 Rv0942 Unknown Hypothetical protein Rv0942, (MTCY10D7.32c), len: 92 aa. Hypothetical unknown protein. P64763 No conserved hypotheticals Mb0967 NC_000962.3 Mycobrowser_v2 CDS 805526 805759 . + 0 Rv0709 rpmC Involved in translation mechanisms. 50S ribosomal protein L29 RpmC Rv0709, (MTCY210.28), len: 77 aa. rpmC, 50S ribosomal protein L29, equivalent to O06050|RL29_MYCBO|MBS10OPER_10 50S ribosomal protein L29 from Mycobacterium bovis BCG (75 aa); and O32989|RL29_MYCLE|MLCB2492_10 50S ribosomal protein L29 from Mycobacterium leprae (80 aa). Also highly similar to others e.g. Q9L0D2|RL29_STRCO 50S ribosomal protein L29 from Streptomyces coelicolor (74 aa); P12873|RL29_BACSU 50s ribosomal protein l29 from Bacillus subtilis (66 aa), FASTA scores: opt: 225, E(): 8.3e-11, (58.6% identity in 58 aa overlap); etc. Belongs to the L29P family of ribosomal proteins. P95057 No information pathways ML1855 MMAR_1039 MSMEG_1444 Mb0729 NC_000962.3 Mycobrowser_v2 CDS 805756 806166 . + 0 Rv0710 rpsQ Protein S17 binds specifically to the 5' end of 16S ribosomal RNA. 30S ribosomal protein S17 RpsQ Rv0710, (MTCY210.29), len: 136 aa. rpsQ, 30S ribosomal protein S17, equivalent to O06051|RS17_MYCBO 30S|MBS10OPER_11 30S ribosomal protein S17 from Mycobacterium bovis BCG (136 aa); and MLCB2492_11 30S ribosomal protein S17 from Mycobacterium leprae (126 aa). Also highly similar to others e.g. CAB82079.1|AL161803 30S ribosomal protein S17 from Streptomyces coelicolor (95 aa); P12874|RS17_BACSU 30s ribosomal protein s17 (bs 16) from Bacillus subtilis (86 aa), FASTA scores: opt: 305, E(): 1.6e-11, (60.5% identity in 81 aa overlap); etc. Contains PS00056 Ribosomal protein S17 signature. P95058 No information pathways ML1854 MMAR_1040 MSMEG_1445 Mb0730 NC_000962.3 Mycobrowser_v2 CDS 806335 808698 . + 0 Rv0711 atsA Thought to play an important role in the mineralization of sulfates [catalytic activity: a phenol sulfate + H2O = a phenol + sulfate]. Possible arylsulfatase AtsA (aryl-sulfate sulphohydrolase) (arylsulphatase) Rv0711, (MTCY210.30), len: 787 aa. Possible atsA, arylsulfatase, similar to others e.g. P51691|ARS_PSEAE arylsulfatase from Pseudomonas aeruginosa (532 aa), FASTA scores: opt: 439, E(): 2.9e-21, (30.8% identity in 552 aa overlap); etc. Also similar to other hypothetical arylsulfatases from Mycobacterium tuberculosis e.g. Rv3299c, Rv0663, etc. Contains PS00523 Sulfatases signature 1, and PS00149 Sulfatases signature 2. Belongs to the sulfatase family. P95059 No intermediary metabolism and respiration ML1853 MMAR_1041 MSMEG_1451 Mb0731,Mb0732 NC_000962.3 Mycobrowser_v2 CDS 811742 812059 . + 0 Rv0715 rplX This protein is found in the ribonucleoprotein core and is involved in the early assembly of the 50S subunit. It is not involved in the functions of the mature 50S subunit. 50S ribosomal protein L24 RplX Rv0715, (MTCY210.34), len: 105 aa. rplX, 50S ribosomal protein L24, equivalent to O32994|MLCB2492_15 50S ribosomal protein L24 from Mycobacterium leprae (105 aa). Also highly similar to others e.g. CAB82081.1|AL161803 50S ribosomal protein L24 from Streptomyces coelicolor (107 aa); P12876|RL24_BACSU 50s ribosomal protein L24 (bl23) from Bacillus subtilis (103 aa), FASTA scores: opt: 363, E(): 1.8e-18, (56.7% identity in 104 aa overlap); etc. Contains PS01108 Ribosomal protein L24 signature. Belongs to the L24P family of ribosomal proteins. P60627 No information pathways ML1848 MMAR_1046 MSMEG_1466 Mb0736 NC_000962.3 Mycobrowser_v2 CDS 812059 812622 . + 0 Rv0716 rplE This is one of 3 proteins that mediate the attachment of the 5S RNA into the large ribosomal subunit. 50S ribosomal protein L5 RplE Rv0716, (MTCY210.35), len: 187 aa. rplE, 50S ribosomal protein L5, equivalent to MLCB2492_16 50S ribosomal protein L5 from Mycobacterium leprae (187 aa). Also highly similar to others e.g. CAB82082.1|AL161803 50S ribosomal protein L5 from Streptomyces coelicolor (185 aa); P33098|RL5_MICLU 50S ribosomal protein L5 from Micrococcus luteus (191 aa), FASTA scores: opt: 930, E(): 0, (73.8% identity in 183 aa overlap); etc. Belongs to the L5P family of ribosomal proteins. P62403 No information pathways ML1847 MMAR_1047 MSMEG_1467 Mb0737 NC_000962.3 Mycobrowser_v2 CDS 812627 812812 . + 0 Rv0717 rpsN1 Known to be required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the a site. 30S ribosomal protein S14 RpsN1 Rv0717, (MTCY210.36), len: 61 aa. rpsN1, 30S ribosomal protein S14, equivalent to MLCB2492_17|O32996 ribosomal protein S14 from Mycobacterium leprae (61 aa). Also highly similar to others e.g. CAB82083.1|AL161803 30S ribosomal protein S14 from Streptomyces coelicolor (61 aa); P24320|RS14_THETH 30s ribosomal protein S14 from Thermus aquaticus (subsp. thermophilus) (60 aa), FASTA scores: opt: 316, E(): 2e-19,(70.0% identity in 60 aa overlap); etc. Contains PS00527 Ribosomal protein S14 signature. Belongs to the S14P family of ribosomal proteins. Note that previously known as rpsN. P0A5X2 No information pathways ML1846 MMAR_1048 MSMEG_1468 Mb0738 NC_000962.3 Mycobrowser_v2 CDS 812976 813374 . + 0 Rv0718 rpsH Binds directly to the central domain of 16S ribosomal RNA. 30S ribosomal protein S8 RpsH Rv0718, (MTCY210.37), len: 132 aa. rpsH, 30S ribosomal protein S8, equivalent to O32997|MLCB2492_18 30S ribosomal protein S8 from Mycobacterium leprae (132 aa). Also highly similar to others e.g. CAB82084.1|AL161803 30S ribosomal protein S8 from Streptomyces coelicolor (132 aa); P33106|RS8_MICLU 30s ribosomal protein S8 from Micrococcus luteus (132 aa), FASTA scores: opt: 669, E(): 0, (77.3% identity in 132 aa overlap); etc. Contains PS00053 Ribosomal protein S8 signature. Belongs to the S8P family of ribosomal proteins. P66625 No information pathways ML1845 MMAR_1049 MSMEG_1469 Mb0739 NC_000962.3 Mycobrowser_v2 CDS 813398 813937 . + 0 Rv0719 rplF This protein binds directly to 23S ribosomal RNA and is located at the aminoacyl-tRNA binding site of the peptidyltransferase center. 50S ribosomal protein L6 RplF Rv0719, (MTCY210.38), len: 179 aa. rplF, 50S ribosomal protein L6, equivalent to O32998|MLCB2492_19 50S ribosomal protein L6 from Mycobacterium leprae (179 aa). Also highly similar to others e.g. P46786|RL6_STRCO|CAB82085.1|AL161803|SCD31.42 50S ribosomal protein L6 from Streptomyces coelicolor (179 aa), FASTA scores: opt: 872, E(): 0, (70.4% identity in 179 aa overlap); etc. Contains PS00525 Ribosomal protein L6 signature 1. Belongs to the L6P family of ribosomal proteins. P66311 No information pathways ML1844 MMAR_1050 MSMEG_1470 Mb0740 NC_000962.3 Mycobrowser_v2 CDS 2250996 2251883 . - 0 Rv2005c Rv2005c Unknown Universal stress protein family protein Rv2005c, (MTCY39.12), len: 295 aa. Universal stress protein family protein. Predicted possible vaccine candidate (See Zvi et al., 2008). P64921 No virulence, detoxification, adaptation ML1421 MMAR_2993 MSMEG_3945 Mb2028c NC_000962.3 Mycobrowser_v2 RBS 2251890 2251893 . - 0 Rv2005c Rv2005c Unknown Universal stress protein family protein Rv2005c, (MTCY39.12), len: 295 aa. Universal stress protein family protein. Predicted possible vaccine candidate (See Zvi et al., 2008). P64921 No virulence, detoxification, adaptation ML1421 MMAR_2993 MSMEG_3945 Mb2028c NC_000962.3 Mycobrowser_v2 CDS 813940 814308 . + 0 Rv0720 rplR This is one of 3 proteins that mediate the attachment of the 5S RNA into the large ribosomal subunit. 50S ribosomal protein L18 RplR Rv0720, (MTCY210.39), len: 122 aa. rplR, 50S ribosomal protein L18, equivalent to O32999|MLCB2492_20|RL18_MYCLE 50S ribosomal protein L18 from Mycobacterium leprae (122 aa). Also highly similar to others e.g. CAB82086.1|AL161803 50S ribosomal protein L18 from Streptomyces coelicolor (127 aa); P33102|RL18_MICLU 50s ribosomal protein L18 from Micrococcus luteus (119 aa), FASTA scores: opt: 447, E(): 8.7e-24, (60.4% identity in 111 aa overlap); etc. Belongs to the L18P family of ribosomal proteins. P66076 No information pathways ML1843 MMAR_1051 MSMEG_1471 Mb0741 NC_000962.3 Mycobrowser_v2 CDS 817531 817866 . - 0 Rv0724A Rv0724A Function unknown Conserved hypothetical protein Rv0724A, len: 111 aa. Similarity suggests that this CDS should be continuation of Rv0725c but we can find no frame-shift to account for this. Possible extended protein is very similar to other hypothetical Mycobacterium tuberculosis proteins e.g. Rv1729c|Z81360_12 (312 aa), FASTA scores: opt: 399, E(): 2e-19, (58.7% identity in 109 aa overlap); Rv0731c, Rv0726c, etc. Frame-shift could occur at nt 817866. Same sequence for strain CDC1551 and Mycobacterium bovis. Q79FX1 No conserved hypotheticals NC_000962.3 Mycobrowser_v2 CDS 814328 814990 . + 0 Rv0721 rpsE Protein S5 is important in the assembly and function of the 30S ribosomal subunit. 30S ribosomal protein S5 RpsE Rv0721, (MTCY210.40), len: 220 aa. rpsE, 30S ribosomal protein S5, equivalent to MLCB2492_21 ribosomal protein S5 from Mycobacterium leprae (217 aa). Also highly similar to others e.g. P46790|RS5_STRCO 30s ribosomal protein S5 from Streptomyces coelicolor (167 aa), FASTA scores: opt: 889, E(): 0, (82.1% identity in 162 aa overlap); etc. Note N-terminus is extented compared to other rpsE genes. Contains PS00585 Ribosomal protein S5 signature, PTS HPr component phosphorylation sites signature. Belongs to the S5P family of ribosomal proteins. P66574 No information pathways ML1842 MMAR_1052 MSMEG_1472 Mb0742 NC_000962.3 Mycobrowser_v2 CDS 814993 815190 . + 0 Rv0722 rpmD Involved ion translation mechanisms. 50S ribosomal protein L30 RpmD Rv0722, (MTCY210.41), len: 65 aa. rpmD, 50S ribosomal protein L30, equivalent to O33001 ribosomal protein L30 from Mycobacterium leprae (71 aa). Also highly similar to others e.g. P46789|RL30_STRCO 50S ribosomal protein L30 from Streptomyces coelicolor (60 aa); P02430|RL30_ECOLI 50S ribosomal protein L30 from Escherichia coli (58 aa), FASTA scores: opt: 168, E(): 1.5e-13, (53.7% identity in 54 aa overlap); etc. Belongs to the L30P family of ribosomal proteins. P66181 No information pathways ML1841 MMAR_1053 MSMEG_1473 Mb0743 NC_000962.3 Mycobrowser_v2 CDS 881459 882418 . + 0 Rv0787 Rv0787 Unknown Unknown protein Rv0787, (MTCY369.31), len: 319 aa. Unknown protein, equivalent to AAK45053.1 from Mycobacterium tuberculosis strain CDC1551 (242 aa) but longer 77 aa. P71840 No conserved hypotheticals ML2220 MMAR_4900 MSMEG_6455 Mb0810 NC_000962.3 Mycobrowser_v2 CDS 815190 815630 . + 0 Rv0723 rplO This protein binds directly to 23S ribosomal RNA. 50S ribosomal protein L15 RplO Rv0723, (MTCY210.42), len: 146 aa. rplO, 50S ribosomal protein L15, equivalent to MLCB2492_23|O33002 50S ribosomal protein L15 from Mycobacterium leprae (146 aa). Also highly similar to others e.g. P46787|RL15_STRCO|SCD31.46 50S ribosomal protein L15 from Streptomyces coelicolor (151 aa); P19946|RL15_BACSU 50s ribosomal protein L15 from Bacillus subtilis (146 aa), FASTA scores: opt: 419, E(): 6.5e-20, (51.0% identity in 145 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00475 Ribosomal protein L15 signature. Belongs to the L15P family of ribosomal proteins. P95071 No information pathways ML1840 MMAR_1054 MSMEG_1474 Mb0744 NC_000962.3 Mycobrowser_v2 RBS 830051 830054 . + 0 Rv0738 Rv0738 Function unknown Conserved protein Rv0738, (MTV041.12), len: 182 aa. Conserved protein, showing weak similarity with hypothetical proteins from Mycobacterium tuberculosis: Rv1727|MTCY04C12.12 (189 aa); MTY13D12_7|Z80343 hypothetical protein from Mycobacterium tuberculosis (194 aa), FASTA scores: opt: 172, E(): 0.0004, (24.2% identity in 178 aa overlap); and C-terminus of Rv0576. O53801 No conserved hypotheticals MMAR_1076 Mb0759 NC_000962.3 Mycobrowser_v2 CDS 830062 830610 . + 0 Rv0738 Rv0738 Function unknown Conserved protein Rv0738, (MTV041.12), len: 182 aa. Conserved protein, showing weak similarity with hypothetical proteins from Mycobacterium tuberculosis: Rv1727|MTCY04C12.12 (189 aa); MTY13D12_7|Z80343 hypothetical protein from Mycobacterium tuberculosis (194 aa), FASTA scores: opt: 172, E(): 0.0004, (24.2% identity in 178 aa overlap); and C-terminus of Rv0576. O53801 No conserved hypotheticals MMAR_1076 Mb0759 NC_000962.3 Mycobrowser_v2 CDS 815663 817534 . + 0 Rv0724 sppA Involved in digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane. Possible protease IV SppA (endopeptidase IV) (signal peptide peptidase) Rv0724, (MTCY210.43), len: 623 aa. Possible sppA, protease IV (endopeptidase IV), equivalent (but longer 23 aa) to MLCB2492_24|O33003 endopeptidase IV from Mycobacterium leprae (602 aa). Also similar to others e.g. NP_419743.1|NC_002696 signal peptide peptidase SppA from Caulobacter crescentus (594 aa); P08395|SPPA_ECOLI|B1766 protease IV (endopeptidase) from Escherichia coli strain K-12 (618 aa), FASTA scores: opt: 582, E(): 8.9e-27, (34.1% identity in 525 aa overlap); etc. Belongs to peptidase family S49. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P95072 No cell wall and cell processes ML1839 MMAR_1056 MSMEG_1476 Mb0745 NC_000962.3 Mycobrowser_v2 CDS 817539 818444 . - 0 Rv0725c Rv0725c Function unknown Conserved hypothetical protein Rv0725c, (MTCY210.44c), len: 301 aa. Conserved hypothetical protein, similar to hypothetical proteins from Mycobacterium tuberculosis e.g. Rv0726c, Rv0731c, Rv3399, etc, e.g. Y893_MYCTU|Q10552|Rv0893C hypothetical 36.1 kDa protein cy31.21c (325 aa), FASTA scores: opt: 600, E(): 3.9e-32, (43.8% identity in 219 aa overlap). P95073 No conserved hypotheticals MMAR_1057 MSMEG_1479,MSMEG_1481 Mb0746c NC_000962.3 Mycobrowser_v2 CDS 818537 819640 . - 0 Rv0726c Rv0726c Possible methyltransferase Possible S-adenosylmethionine-dependent methyltransferase Rv0726c, (MTCY210.45c), len: 367 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), highly similar to other proteins from Mycobacterium tuberculosis e.g. Q10552|Y893_MYCTU|Rv0893c|MT0917|MTCY31.21c (325 aa), FASTA scores: opt: 646, E(): 0, (38.3% identity in 329 aa overlap); Rv0731c|MTV041.05c (318 aa), Rv3399, etc. Also similar to proteins from Mycobacterium leprae and other organisms e.g. T35930 hypothetical protein SC9B5.10 from Streptomyces coelicolor (303 aa). P95074 No lipid metabolism MMAR_1059 Mb0747c NC_000962.3 Mycobrowser_v2 CDS 819843 820499 . - 0 Rv0727c fucA Involved in fucose metabolism (at the third step) [catalytic activity: L-fuculose 1-phosphate = glycerone phosphate + (S)-lactaldehyde]. Possible L-fuculose phosphate aldolase FucA (L-fuculose-1-phosphate aldolase) Rv0727c, (MTV41.01c, MTCY210.46c), len: 218 aa. Possible fucA, L-fuculose-1-phosphate aldolase, similar to many e.g. NP_386339.1|NC_003047 putative L-fuculose phosphate aldolase protein from Sinorhizobium meliloti (222 aa); P11550|FUCA_ECOLI L-fuculose phosphate aldolase from Escherichia strain K12 (215 aa), FASTA scores: opt: 372, E(): 4.1e-19, (34.6% identity in 185 aa overlap); etc. Belongs to the aldolase class II family, ARAD/FUCA subfamily. Cofactor: binds one zinc ion per molecule. P95075 No intermediary metabolism and respiration ML1838 MMAR_1064 Mb0748c NC_000962.3 Mycobrowser_v2 RBS 820510 820513 . - 0 Rv0727c fucA Involved in fucose metabolism (at the third step) [catalytic activity: L-fuculose 1-phosphate = glycerone phosphate + (S)-lactaldehyde]. Possible L-fuculose phosphate aldolase FucA (L-fuculose-1-phosphate aldolase) Rv0727c, (MTV41.01c, MTCY210.46c), len: 218 aa. Possible fucA, L-fuculose-1-phosphate aldolase, similar to many e.g. NP_386339.1|NC_003047 putative L-fuculose phosphate aldolase protein from Sinorhizobium meliloti (222 aa); P11550|FUCA_ECOLI L-fuculose phosphate aldolase from Escherichia strain K12 (215 aa), FASTA scores: opt: 372, E(): 4.1e-19, (34.6% identity in 185 aa overlap); etc. Belongs to the aldolase class II family, ARAD/FUCA subfamily. Cofactor: binds one zinc ion per molecule. P95075 No intermediary metabolism and respiration ML1838 MMAR_1064 Mb0748c NC_000962.3 Mycobrowser_v2 CDS 820496 821476 . - 0 Rv0728c serA2 Involved at the first committed step in the 'phosphorylated' pathway of L-serine biosynthesis. Catalyzes the oxidation of D-3-phosphoglycerate to 3-phosphohydroxypyruvate [catalytic activity: 3-phosphoglycerate + NAD(+) = 3-phosphohydroxypyruvate + NADH]. Possible D-3-phosphoglycerate dehydrogenase SerA2 (phosphoglycerate dehydrogenase) (PGDH) Rv0728c, (MTV041.02c), len: 326 aa. Possible serA2, D-3-phosphoglycerate dehydrogenase, similar to others e.g. AF0278|AF027868_5|YoaD D-3-phosphoglycerate dehydrogenase from Bacillus subtilis (344 aa), FASTA scores: opt: 594, E(): 3.1e-31, (35.9% identity in 309 aa overlap); etc. Also similar to Rv2996c|MTV012.10|SERA1 D-3-phosphoglycerate dehydrogenase from Mycobacterium tuberculosis (528 aa). O86322 No intermediary metabolism and respiration ML1837 MMAR_1065 MSMEG_0604 Mb0749c NC_000962.3 Mycobrowser_v2 CDS 2314825 2315061 . - 0 Rv2058c rpmB2 Involved in ribosome activity 50S ribosomal protein L28 RpmB2 Rv2058c, (MTCY63A.02), len: 78 aa. rpmB2, 50S ribosomal protein L28, very similar to rL28 of M. tuberculosis. Also similar to rpmB (Rv0105c) of Mycobacterium tuberculosis. P66148 No information pathways MSMEG_6068 Mb2084c NC_000962.3 Mycobrowser_v2 RBS 2315072 2315076 . - 0 Rv2058c rpmB2 Involved in ribosome activity 50S ribosomal protein L28 RpmB2 Rv2058c, (MTCY63A.02), len: 78 aa. rpmB2, 50S ribosomal protein L28, very similar to rL28 of M. tuberculosis. Also similar to rpmB (Rv0105c) of Mycobacterium tuberculosis. P66148 No information pathways MSMEG_6068 Mb2084c NC_000962.3 Mycobrowser_v2 RBS 827531 827535 . + 0 Rv0735 sigL The sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released. Probable alternative RNA polymerase sigma factor SigL Rv0735, (MTV041.09), len: 177 aa. Probable sigL, alternative RNA polymerase sigma factor (rpoE) (see citations below), highly similar to many proteins of the extracytoplasmatic function (ECF) subfamily e.g. CAB72200.1|AL138851 putative RNA polymerase sigma factor from Streptomyces coelicolor (194 aa); Q06909|CARQ_MYXXA RNA polymerase sigma factor CARQ from Myxococcus xanthus (174 aa), FASTA scores: opt: 251, E(): 9.6e-11, (32.9% identity in 161 aa overlap); etc. Also similar to MTCI61_4, MTU87242_1, and MLU15180_30 from Mycobacterium tuberculosis. Contains PS01063 Sigma-70 factors ECF subfamily signature and probable helix-turn helix motif from aa 139-160 (Score 1134, +3.05 SD). Belongs to the sigma-70 factor family, ECF subfamily. Q7D9D4 No information pathways MMAR_1073 MSMEG_1486 Mb0756 NC_000962.3 Mycobrowser_v2 CDS 827543 828076 . + 0 Rv0735 sigL The sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released. Probable alternative RNA polymerase sigma factor SigL Rv0735, (MTV041.09), len: 177 aa. Probable sigL, alternative RNA polymerase sigma factor (rpoE) (see citations below), highly similar to many proteins of the extracytoplasmatic function (ECF) subfamily e.g. CAB72200.1|AL138851 putative RNA polymerase sigma factor from Streptomyces coelicolor (194 aa); Q06909|CARQ_MYXXA RNA polymerase sigma factor CARQ from Myxococcus xanthus (174 aa), FASTA scores: opt: 251, E(): 9.6e-11, (32.9% identity in 161 aa overlap); etc. Also similar to MTCI61_4, MTU87242_1, and MLU15180_30 from Mycobacterium tuberculosis. Contains PS01063 Sigma-70 factors ECF subfamily signature and probable helix-turn helix motif from aa 139-160 (Score 1134, +3.05 SD). Belongs to the sigma-70 factor family, ECF subfamily. Q7D9D4 No information pathways MMAR_1073 MSMEG_1486 Mb0756 NC_000962.3 Mycobrowser_v2 CDS 821507 822853 . + 0 Rv0729 xylB Phosphorylates D-xylulose [catalytic activity: ATP + D-xylulose = ADP + D-xylulose 5-phosphate]. Possible D-xylulose kinase XylB (xylulokinase) (xylulose kinase) Rv0729, (MTV041.03), len: 448 aa. Possible xylB, D-xylulose-kinase (xylulokinase). C-terminus highly similar to AAD09880.1|U77912 unknown protein from Mycobacterium bovis (102 aa); and N-terminus highly similar to T45387|Z98756|MLCB2492_25 hypothetical protein from Mycobacterium leprae (110 aa), FASTA scores: opt: 427, E(): 1.1e-19, (60.9% identity in 110 aa overlap). Also similar to xylA/xylB genes from various bacterial species e.g. AAC26499.1|AF045245 D-xylulose-kinase from Klebsiella pneumoniae (487 aa); NP_418021.1|NC_000913 xylulokinase from Escherichia coli strain K12 (484 aa), FASTA scores: opt: 260, E(): 7.5e-09, (25.9% identity in 478 aa overlap); etc. Also similar to Rv3696c|glpK probable glycerol kinase from Mycobacterium tuberculosis (517 aa). Belongs to the fucokinase / gluconokinase / glycerokinase / xylulokinase family. O53793 No intermediary metabolism and respiration ML1836 MMAR_1066 MSMEG_3113,MSMEG_3263 Mb0750 NC_000962.3 Mycobrowser_v2 RBS 822857 822860 . + 0 Rv0730 Rv0730 Acetylation, substrate unknown GCN5-related N-acetyltransferase Rv0730, (MTV041.04), len: 242 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain in C-terminal part. See Vetting et al. 2005. Equivalent to Z98756|MLCB2492_26 hypothetical protein from Mycobacterium leprae (227 aa), FASTA scores: opt: 1180, E(): 0, (83.5% identity in 218 aa overlap). Q7D9D7 No intermediary metabolism and respiration ML1835 MMAR_1067 MSMEG_6565 Mb0751 NC_000962.3 Mycobrowser_v2 CDS 822866 823594 . + 0 Rv0730 Rv0730 Acetylation, substrate unknown GCN5-related N-acetyltransferase Rv0730, (MTV041.04), len: 242 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain in C-terminal part. See Vetting et al. 2005. Equivalent to Z98756|MLCB2492_26 hypothetical protein from Mycobacterium leprae (227 aa), FASTA scores: opt: 1180, E(): 0, (83.5% identity in 218 aa overlap). Q7D9D7 No intermediary metabolism and respiration ML1835 MMAR_1067 MSMEG_6565 Mb0751 NC_000962.3 Mycobrowser_v2 CDS 823683 824639 . - 0 Rv0731c Rv0731c Possible methyltransferase Possible S-adenosylmethionine-dependent methyltransferase Rv0731c, (MTV041.05c), len: 318 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), highly similar to other proteins from Mycobacterium tuberculosis e.g. Rv0726c|MTCY210.45c (367 aa), FASTA score: (60.9% identity in 317 aa overlap); Rv3399, Rv1729c, etc. Q7D9D6 No lipid metabolism ML1834 MMAR_1069 Mb0752c NC_000962.3 Mycobrowser_v2 RBS 824646 824651 . - 0 Rv0731c Rv0731c Possible methyltransferase Possible S-adenosylmethionine-dependent methyltransferase Rv0731c, (MTV041.05c), len: 318 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), highly similar to other proteins from Mycobacterium tuberculosis e.g. Rv0726c|MTCY210.45c (367 aa), FASTA score: (60.9% identity in 317 aa overlap); Rv3399, Rv1729c, etc. Q7D9D6 No lipid metabolism ML1834 MMAR_1069 Mb0752c NC_000962.3 Mycobrowser_v2 RBS 824789 824794 . + 0 Rv0732 secY Essential for protein export. Interacts with SECA|Rv3240c and SECE|Rv0638 to allow the translocation of proteins across the plasma membrane, by forming part of a channel. Probable preprotein translocase SecY Rv0732, (MTV041.06), len: 441 aa. Probable SecY, preprotein translocase (integral membrane protein) (see citation below), equivalent to NP_302243.1|NC_002677 SecY subunit of preprotein translocase from Mycobacterium leprae (438 aa); AAC04389.1|AF047021 preprotein translocase subunit from Mycobacterium smegmatis (438 aa); and U77912|MBU77912_1 preprotein translocase subunit from Mycobacterium bovis (441 aa), FASTA scores: opt: 2802, E(): 0, (99.8% identity in 441 aa overlap). Also highly similar to others e.g. P46785|SECY_STRCO preprotein translocase SECY subunit from Streptomyces coelicolor (437 aa); etc. Contains PS00755 and PS00756 protein secY signatures 1 and 2. Belongs to the SECE/SEC61-alpha family. Part of the prokaryotic protein translocation apparatus which comprise SECA|Rv3240c, SECD|Rv2587c, SECE|Rv0638, SECF|Rv2586c, SECG|Rv1440 and SECY. P0A5Z2 No cell wall and cell processes ML1833 MMAR_1070 MSMEG_1483 Mb0753 NC_000962.3 Mycobrowser_v2 CDS 824800 826125 . + 0 Rv0732 secY Essential for protein export. Interacts with SECA|Rv3240c and SECE|Rv0638 to allow the translocation of proteins across the plasma membrane, by forming part of a channel. Probable preprotein translocase SecY Rv0732, (MTV041.06), len: 441 aa. Probable SecY, preprotein translocase (integral membrane protein) (see citation below), equivalent to NP_302243.1|NC_002677 SecY subunit of preprotein translocase from Mycobacterium leprae (438 aa); AAC04389.1|AF047021 preprotein translocase subunit from Mycobacterium smegmatis (438 aa); and U77912|MBU77912_1 preprotein translocase subunit from Mycobacterium bovis (441 aa), FASTA scores: opt: 2802, E(): 0, (99.8% identity in 441 aa overlap). Also highly similar to others e.g. P46785|SECY_STRCO preprotein translocase SECY subunit from Streptomyces coelicolor (437 aa); etc. Contains PS00755 and PS00756 protein secY signatures 1 and 2. Belongs to the SECE/SEC61-alpha family. Part of the prokaryotic protein translocation apparatus which comprise SECA|Rv3240c, SECD|Rv2587c, SECE|Rv0638, SECF|Rv2586c, SECG|Rv1440 and SECY. P0A5Z2 No cell wall and cell processes ML1833 MMAR_1070 MSMEG_1483 Mb0753 NC_000962.3 Mycobrowser_v2 CDS 1595979 1596884 . + 0 Rv1421 Rv1421 Function unknown Conserved protein Rv1421, (MTCY21B4.39), len: 301 aa. Conserved protein, similar to many hypothetical proteins e.g. YHBJ_ECOLI|P33995 hypothetical 32.5 kd protein from Escherichia coli (284 aa), FASTA scores: opt: 648, E(): 6.3e-36, (38.7% identity in 282aa overlap). P67106 No conserved hypotheticals ML0563 MMAR_2228 MSMEG_3079 Mb1456 NC_000962.3 Mycobrowser_v2 RBS 826109 826112 . + 0 Rv0733 adk This small ubiquitous enzyme is essential in intracellular nucleotide metabolism, in addition it has been found to act as both a nucleoside mono- and DI-phosphate kinase suggesting it may have a role in RNA and DNA biosynthesis [catalytic activity: ATP + AMP = ADP + ADP]. Adenylate kinase Adk (ATP-AMP transphosphorylase) Rv0733, (MTV041.07), len: 181 aa. adk, adenylate kinase (ATP-AMP transphosphorylase), equivalent to Z98756|MLCB24 92_28 probable adenylate kinase from Mycobacterium leprae (181 aa), FASTA scores: opt: 978, E(): 0, (83.6% identity in 177 aa overlap); and AAF86323.1|AF271342 putative adenylate kinase from Mycobacterium marinum (124 aa) (N-terminus shorter). Also highly similar to others e.g. P43414|KAD_STRCO adenylate kinase from Streptomyces coelicolor (217 aa), FASTA score: (43.0% identity in 186 aa overlap); etc. Contains PS00113 Adenylate kinase signature. Belongs to the adenylate kinase family. P69440 No intermediary metabolism and respiration ML1832 MMAR_1071 MSMEG_1484 Mb0754 NC_000962.3 Mycobrowser_v2 CDS 826122 826667 . + 0 Rv0733 adk This small ubiquitous enzyme is essential in intracellular nucleotide metabolism, in addition it has been found to act as both a nucleoside mono- and DI-phosphate kinase suggesting it may have a role in RNA and DNA biosynthesis [catalytic activity: ATP + AMP = ADP + ADP]. Adenylate kinase Adk (ATP-AMP transphosphorylase) Rv0733, (MTV041.07), len: 181 aa. adk, adenylate kinase (ATP-AMP transphosphorylase), equivalent to Z98756|MLCB24 92_28 probable adenylate kinase from Mycobacterium leprae (181 aa), FASTA scores: opt: 978, E(): 0, (83.6% identity in 177 aa overlap); and AAF86323.1|AF271342 putative adenylate kinase from Mycobacterium marinum (124 aa) (N-terminus shorter). Also highly similar to others e.g. P43414|KAD_STRCO adenylate kinase from Streptomyces coelicolor (217 aa), FASTA score: (43.0% identity in 186 aa overlap); etc. Contains PS00113 Adenylate kinase signature. Belongs to the adenylate kinase family. P69440 No intermediary metabolism and respiration ML1832 MMAR_1071 MSMEG_1484 Mb0754 NC_000962.3 Mycobrowser_v2 RBS 826659 826664 . + 0 Rv0734 mapA Removes the amino-terminal methionine from nascent proteins [catalytic activity: L-methionylpeptide + H2O = L-methionine + peptide]. Methionine aminopeptidase MapA (map) (peptidase M) (MetAP) Rv0734, (MTV041.08), len: 266 aa. mapA, methionine aminopeptidase (map), equivalent to Z98756|MLCB2492_29 probable methionine aminopeptidase from Mycobacterium leprae (266 aa), FASTA scores: opt: 1717, E(): 0, (83.4% identity in 265 aa overlap). Also highly similar to many e.g. T35553 methionine aminopeptidase from Streptomyces coelicolor (278 aa); etc. Also similar to Rv2861c|MAPB probable methionine aminopeptidase from Mycobacterium tuberculosis (285 aa). Belongs to peptidase family M24A; also known as the map family 1. Cofactor: cobalt; binds 2 ions per subunit. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). Q7D9D5 No intermediary metabolism and respiration ML1831 MMAR_1072 MSMEG_1485 Mb0755 NC_000962.3 Mycobrowser_v2 CDS 826670 827470 . + 0 Rv0734 mapA Removes the amino-terminal methionine from nascent proteins [catalytic activity: L-methionylpeptide + H2O = L-methionine + peptide]. Methionine aminopeptidase MapA (map) (peptidase M) (MetAP) Rv0734, (MTV041.08), len: 266 aa. mapA, methionine aminopeptidase (map), equivalent to Z98756|MLCB2492_29 probable methionine aminopeptidase from Mycobacterium leprae (266 aa), FASTA scores: opt: 1717, E(): 0, (83.4% identity in 265 aa overlap). Also highly similar to many e.g. T35553 methionine aminopeptidase from Streptomyces coelicolor (278 aa); etc. Also similar to Rv2861c|MAPB probable methionine aminopeptidase from Mycobacterium tuberculosis (285 aa). Belongs to peptidase family M24A; also known as the map family 1. Cofactor: cobalt; binds 2 ions per subunit. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). Q7D9D5 No intermediary metabolism and respiration ML1831 MMAR_1072 MSMEG_1485 Mb0755 NC_000962.3 Mycobrowser_v2 CDS 2546102 2546431 . + 0 Rv2273 Rv2273 Unknown Probable conserved transmembrane protein Rv2273, (MTCY339.37c), len: 109 aa. Probable conserved transmembrane protein, similar to Rv2272 (MTCY339.38c), (35.0% identity in 100 aa overlap). P64971 No cell wall and cell processes ML1790 MMAR_3369 MSMEG_1230 Mb2296 NC_000962.3 Mycobrowser_v2 CDS 829207 829704 . + 0 Rv0737 Rv0737 Involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0737, (MTV041.11), len: 165 aa. Possible transcriptional regulator, similar to others e.g. BAB69161.1|AB070937 regulator protein from Streptomyces avermitilis (169 aa); NP_419731.1|NC_002696 transcriptional regulator MarR family from Caulobacter crescentus (148 aa) (homology only at C-terminus); etc. Also shows weak similarity to AB0014|AB001488_14 hypothetical protein from Bacillus subtilis (164 aa), FASTA scores: opt: 163, E(): 9.3e-05, (32.8% identity in 116 aa overlap), which is similar to slyY gene of S. typhimurium required for survival in macrophage. Contains possible helix-turn helix motif from aa 73-94 (Score 1138, +3.06 SD). Q7D9D2 No regulatory proteins MMAR_1075 MSMEG_1492 Mb0758 NC_000962.3 Mycobrowser_v2 CDS 2291269 2291925 . + 0 Rv2046 lppI Unknown Probable lipoprotein LppI Rv2046, (MTV018.33), len: 218 aa. Probable lppI, lipoprotein contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O53489 No cell wall and cell processes ML1435 MMAR_3021 MSMEG_3851 Mb2072 NC_000962.3 Mycobrowser_v2 CDS 830855 831661 . + 0 Rv0739 Rv0739 Function unknown Conserved hypothetical protein Rv0739, (MTV041.13), len: 268 aa. Conserved hypothetical protein, showing some similarity to Mycobacterium tuberculosis proteins Rv0026 (448 aa), FASTA score: (37.6% identity in 101 aa overlap)and Rv0025 (120 aa), FASTA score: (32.4% identity in 142 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53802 No conserved hypotheticals Mb0760 NC_000962.3 Mycobrowser_v2 CDS 831776 832303 . + 0 Rv0740 Rv0740 Function unknown Conserved hypothetical protein Rv0740, (MTV041.14), len: 175 aa. Conserved hypothetical protein; C-terminus (possibly part of truncated IS1557) shows nearly perfect identity to Rv0750|MTV041_24 (81 aa), FASTA score: (92.6% identity in 81 aa overlap). Also shows weak similarity to MTV007_5 hypothetical protein from Mycobacterium tuberculosis (313 aa), FASTA score: (34.5% identity in 110 aa overlap); and MLCL536_27 hypothetical protein from Mycobacterium leprae (315 aa), FASTA score: (34.5% identity in 84 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53803 No conserved hypotheticals Mb0761 NC_000962.3 Mycobrowser_v2 CDS 832534 832848 . + 0 Rv0741 Rv0741 Required for the transposition of the insertion element IS1557. Probable transposase (fragment) Rv0741, (MTV041.15), len: 104 aa. Probable truncated transposase for IS1557, showing similarity to transposases and is elements e.g. U63997|EFU63997_1 insertion sequence from Enterococcus faecium (424 aa), FASTA score: (31.0% identity in 87 aa overlap). Very high similarity with the C-terminal part of Z73419|MTCY373_3 2 IS1557 from Mycobacterium tuberculosis (444 aa), FASTA score: (86.5% identity in 104 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53804 No insertion seqs and phages Mb0762 NC_000962.3 Mycobrowser_v2 RBS 832970 832974 . + 0 Rv0742 PE_PGRS8 Function unknown PE-PGRS family protein PE_PGRS8 Rv0742, (MTV041.16), len: 175 aa. PE_PGRS8, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), similar to many Mycobacterium tuberculosis PGRS-type proteins e.g. Z78020|MTCY1A11_25 (498 aa), FASTA scores: opt: 766, E(): 6.1e-25, (73.6% identity in 178 aa overlap). Similarity suggests ORF starts with ATA start codon. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q79FW9 No PE/PPE Mb0763 NC_000962.3 Mycobrowser_v2 CDS 832981 833508 . + 0 Rv0742 PE_PGRS8 Function unknown PE-PGRS family protein PE_PGRS8 Rv0742, (MTV041.16), len: 175 aa. PE_PGRS8, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), similar to many Mycobacterium tuberculosis PGRS-type proteins e.g. Z78020|MTCY1A11_25 (498 aa), FASTA scores: opt: 766, E(): 6.1e-25, (73.6% identity in 178 aa overlap). Similarity suggests ORF starts with ATA start codon. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q79FW9 No PE/PPE Mb0763 NC_000962.3 Mycobrowser_v2 CDS 833886 834443 . - 0 Rv0743c Rv0743c Unknown Hypothetical protein Rv0743c, (MTV041.17c), len: 185 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53806 No conserved hypotheticals Mb0764c NC_000962.3 Mycobrowser_v2 CDS 834440 834946 . - 0 Rv0744c Rv0744c Thought to be involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0744c, (MTV041.18c), len: 168 aa. Possible transcriptional regulator, showing weak similarity with O86661|SC4A2.05 putative two-component sensor from Streptomyces coelicolor (436 aa), FASTA scores: opt: 117, E(): 0.88, (37.25% identity in 94 aa overlap); and some putative excisionases or transposases. Also weakly similar to P71902|YN10_MYCTU|Rv2310|MT2372|MTCY3G12.24c conserved hypothetical protein from Mycobacterium tuberculosis (114 aa); and Q11144|Y477_MYCTU|Rv0477|MT0495|MTCY20G9.03 conserved hypothetical protein from Mycobacterium tuberculosis (148 aa). Equivalent to AAK45006 from Mycobacterium tuberculosis strain CDC1551 (179 aa) but shorter 11 aa. Contains probable helix-turn helix motif from aa 5-26 (Score 1350, +3.78 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53807 No regulatory proteins Mb0765c NC_000962.3 Mycobrowser_v2 CDS 2314354 2314659 . - 0 Rv2056c rpsN2 Involved in translation 30S ribosomal protein S14 RpsN2 Rv2056c, (MTCY63A.04), len: 101 aa. rpsN2, 30S ribosomal protein S14, similar to many. Also similar to rpsN|Rv0717|MTCY210.36 from Mycobacterium tuberculosis, (50.0% identity in 62 aa overlap). P66405 No information pathways MMAR_0290 MSMEG_6066 Mb2082c NC_000962.3 Mycobrowser_v2 RBS 2314669 2314672 . - 0 Rv2056c rpsN2 Involved in translation 30S ribosomal protein S14 RpsN2 Rv2056c, (MTCY63A.04), len: 101 aa. rpsN2, 30S ribosomal protein S14, similar to many. Also similar to rpsN|Rv0717|MTCY210.36 from Mycobacterium tuberculosis, (50.0% identity in 62 aa overlap). P66405 No information pathways MMAR_0290 MSMEG_6066 Mb2082c NC_000962.3 Mycobrowser_v2 CDS 1419014 1419748 . - 0 Rv1270c lprA Unknown Possible lipoprotein LprA Rv1270c, (MTCY50.12), len: 244 aa. Possible lprA, lipoprotein. Similar to O32852|AJ000500 lipoprotein from Mycobacterium bovis (236 aa), fasta scores: E(): 5.2e-23, (35.1% identity in 245 aa overlap). Similar to M. tuberculosis lipoproteins: Rv1368, Rv1411c, Rv2945c. Contains probable N-terminal signal sequence. Q11049 No cell wall and cell processes MMAR_4152 Mb1301c NC_000962.3 Mycobrowser_v2 RBS 1419755 1419759 . - 0 Rv1270c lprA Unknown Possible lipoprotein LprA Rv1270c, (MTCY50.12), len: 244 aa. Possible lprA, lipoprotein. Similar to O32852|AJ000500 lipoprotein from Mycobacterium bovis (236 aa), fasta scores: E(): 5.2e-23, (35.1% identity in 245 aa overlap). Similar to M. tuberculosis lipoproteins: Rv1368, Rv1411c, Rv2945c. Contains probable N-terminal signal sequence. Q11049 No cell wall and cell processes MMAR_4152 Mb1301c NC_000962.3 Mycobrowser_v2 CDS 835154 835681 . + 0 Rv0745 Rv0745 Function unknown Conserved hypothetical protein Rv0745, (MTV041.19), len: 175 aa. Conserved hypothetical protein; shows high similarity to a 50 aa region of Rv3649|Z95436|MTY15C10_3 conserved hypothetical protein, similar to ATP-dependent helicases, from Mycobacterium tuberculosis (771 aa), FASTA scores: opt: 225, E(): 7e-06, (70.0% identity in 50 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53808 No conserved hypotheticals Mb0766 NC_000962.3 Mycobrowser_v2 RBS 835690 835694 . + 0 Rv0746 PE_PGRS9 Function unknown PE-PGRS family protein PE_PGRS9 Rv0746, (MTV041.20), len: 783 aa. PE_PGRS9, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to part of MTCY28.25c|Rv1759c|Z95890 antigen wag22 from M. tuberculosis (914 aa), FASTA scores: opt: 2429, E(): 0, (56.9% identity in 873 aa overlap). Also similar to other PE-PGRS family proteins e.g. AL0212|MTV008_46 FASTA score: (48.8% identity in 887 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q79FW8 No PE/PPE MMAR_1195,MMAR_1391,MMAR_1850,MMAR_1851,MMAR_2695,MMAR_2948,MMAR_3326,MMAR_4398,MMAR_5207 Mb0767 NC_000962.3 Mycobrowser_v2 CDS 835701 838052 . + 0 Rv0746 PE_PGRS9 Function unknown PE-PGRS family protein PE_PGRS9 Rv0746, (MTV041.20), len: 783 aa. PE_PGRS9, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to part of MTCY28.25c|Rv1759c|Z95890 antigen wag22 from M. tuberculosis (914 aa), FASTA scores: opt: 2429, E(): 0, (56.9% identity in 873 aa overlap). Also similar to other PE-PGRS family proteins e.g. AL0212|MTV008_46 FASTA score: (48.8% identity in 887 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q79FW8 No PE/PPE MMAR_1195,MMAR_1391,MMAR_1850,MMAR_1851,MMAR_2695,MMAR_2948,MMAR_3326,MMAR_4398,MMAR_5207 Mb0767 NC_000962.3 Mycobrowser_v2 RBS 838440 838444 . + 0 Rv0747 PE_PGRS10 Function unknown PE-PGRS family protein PE_PGRS10 Rv0747, (MTV041.21), len: 801 aa. PE_PGRS10, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to part of MTCY28.25c|Rv1759c|Z95890 antigen wag22 from M. tuberculosis (914 aa), FASTA scores: opt: 2772, E(): 0, (60.9% identity in 941 aa overlap). Also similar to other PE-PGRS family proteins e.g. Z95844|MTCY493_2 FASTA score: (50.2% identity in 815 aa overlap). Contains PS00012 Phosphopantetheine attachment site. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53810 No PE/PPE Mb0768 NC_000962.3 Mycobrowser_v2 CDS 838451 840856 . + 0 Rv0747 PE_PGRS10 Function unknown PE-PGRS family protein PE_PGRS10 Rv0747, (MTV041.21), len: 801 aa. PE_PGRS10, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to part of MTCY28.25c|Rv1759c|Z95890 antigen wag22 from M. tuberculosis (914 aa), FASTA scores: opt: 2772, E(): 0, (60.9% identity in 941 aa overlap). Also similar to other PE-PGRS family proteins e.g. Z95844|MTCY493_2 FASTA score: (50.2% identity in 815 aa overlap). Contains PS00012 Phosphopantetheine attachment site. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53810 No PE/PPE Mb0768 NC_000962.3 Mycobrowser_v2 CDS 840947 841204 . + 0 Rv0748 vapB31 Unknown Possible antitoxin VapB31 Rv0748, (MTV041.22), len: 85 aa. Possible vapB31, antitoxin, part of toxin-antitoxin (TA) operon with Rv0749, see Arcus et al. 2005. Also similar to others in Mycobacterium tuberculosis proteins e.g. Rv2871 (75 aa); Rv1241, Rv2132, Rv3321c, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53811 No virulence, detoxification, adaptation Mb0769 NC_000962.3 Mycobrowser_v2 CDS 841228 841656 . + 0 Rv0749 vapC31 Unknown Possible toxin VapC31. Contains PIN domain. Rv0749, (MTV041.23), len: 142 aa. Possible vapC31, toxin, part of toxin-antitoxin (TA) operon with Rv0748, contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. Rv0277c, Rv2530c, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53812 No virulence, detoxification, adaptation Mb0770 NC_000962.3 Mycobrowser_v2 CDS 841737 841874 . - 0 Rv0749A Rv0749A Unknown Hypothetical protein (fragment) Rv0749A, len: 45 aa. Conserved hypothetical protein (probably gene fragment), similar to part (aa 250-292) of Rv2807|Z81331_12 from Mycobacterium tuberculosis (384 aa), FASTA scores: opt: 238, E(): 1.9e-13, (79.07% identity in 43 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q8VKE7 No conserved hypotheticals Mb0771c NC_000962.3 Mycobrowser_v2 CDS 842347 843231 . - 0 Rv0751c mmsB Catalyzes the NAD-dependent, reversible oxidation of 3-hydroxbutyrate to methylmalonate [catalytic activity: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH]. Probable 3-hydroxyisobutyrate dehydrogenase MmsB (hibadh) Rv0751c, (MTV041.25c), len: 294 aa. Probable mmsB, 3-hydroxyisobutyrate dehydrogenase, highly similar to others e.g. NP_102847.1|NC_002678 3-hydroxyisobutyrate dehydrogenase from Mesorhizobium loti (294 aa); NP_420167.1|NC_002696 3-hydroxyisobutyrate dehydrogenase from Caulobacter crescentus (298 aa); A32867 3-hydroxyisobutyrate dehydrogenase from Rattus norvegicus (346 aa); etc. Also similar to methylmalonate semialdehyde dehydrogenases e.g. M84911|PSE MMSRAB_3 methylmalonate semialdehyde dehydrogenase from Pseudomonas aeruginosa (298 aa), FASTA scores: opt: 786, E(): 0, (45.8% identity in 297 aa overlap). Also similar to 6-phosphogluconate dehydrogenases from Mycobacterium tuberculosis e.g. Rv1122 and Rv1844c. Contains PS00895 3-hydroxyisobutyrate dehydrogenase signature. Belongs to the 3-hydroxyisobutyrate dehydrogenase family. P63935 No intermediary metabolism and respiration MMAR_1077 MSMEG_1496 Mb0773c NC_000962.3 Mycobrowser_v2 RBS 843237 843240 . - 0 Rv0751c mmsB Catalyzes the NAD-dependent, reversible oxidation of 3-hydroxbutyrate to methylmalonate [catalytic activity: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH]. Probable 3-hydroxyisobutyrate dehydrogenase MmsB (hibadh) Rv0751c, (MTV041.25c), len: 294 aa. Probable mmsB, 3-hydroxyisobutyrate dehydrogenase, highly similar to others e.g. NP_102847.1|NC_002678 3-hydroxyisobutyrate dehydrogenase from Mesorhizobium loti (294 aa); NP_420167.1|NC_002696 3-hydroxyisobutyrate dehydrogenase from Caulobacter crescentus (298 aa); A32867 3-hydroxyisobutyrate dehydrogenase from Rattus norvegicus (346 aa); etc. Also similar to methylmalonate semialdehyde dehydrogenases e.g. M84911|PSE MMSRAB_3 methylmalonate semialdehyde dehydrogenase from Pseudomonas aeruginosa (298 aa), FASTA scores: opt: 786, E(): 0, (45.8% identity in 297 aa overlap). Also similar to 6-phosphogluconate dehydrogenases from Mycobacterium tuberculosis e.g. Rv1122 and Rv1844c. Contains PS00895 3-hydroxyisobutyrate dehydrogenase signature. Belongs to the 3-hydroxyisobutyrate dehydrogenase family. P63935 No intermediary metabolism and respiration MMAR_1077 MSMEG_1496 Mb0773c NC_000962.3 Mycobrowser_v2 CDS 2258854 2259285 . - 0 Rv2011c Rv2011c Unknown Conserved hypothetical protein, probable transcription repressor. Rv2011c, (MTCY39.06), len: 143 aa. Conserved hypothetical protein, probable transcription repressor. Contains IPR011991 Winged helix-turn-helix transcription repressor DNA-binding domain. P64927 No regulatory proteins Mb2034c NC_000962.3 Mycobrowser_v2 RBS 2259290 2259294 . - 0 Rv2011c Rv2011c Unknown Conserved hypothetical protein, probable transcription repressor. Rv2011c, (MTCY39.06), len: 143 aa. Conserved hypothetical protein, probable transcription repressor. Contains IPR011991 Winged helix-turn-helix transcription repressor DNA-binding domain. P64927 No regulatory proteins Mb2034c NC_000962.3 Mycobrowser_v2 CDS 843242 844414 . - 0 Rv0752c fadE9 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE9 Rv0752c, (MTV041.26c), len: 390 aa. Probable fadE9, acyl-CoA dehydrogenase, highly similar to many e.g. NP_437985.1|NC_003078 putative acyl-CoA dehydrogenase protein from Sinorhizobium meliloti (380 aa); Z99123|BSUB0020_14 from Bacillus subtilis (379 aa), FASTA scores: opt: 853, E(): 0, (39.8% identity in 384 aa overlap); etc. Contains PS00072 Acyl-CoA dehydrogenases signature 1, and PS00073 Acyl-Co Adehydrogenases signature 2. Belongs to the acyl-CoA dehydrogenases family. O53815 No lipid metabolism MMAR_1078 MSMEG_1497 Mb0774c NC_000962.3 Mycobrowser_v2 CDS 844421 845953 . - 0 Rv0753c mmsA Plays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA [catalytic activity: 2-methyl-3-oxopropanoate + CoA + NAD+ = propanoyl-CoA + CO2 + NADH]. Probable methylmalonate-semialdehyde dehydrogenase MmsA (methylmalonic acid semialdehyde dehydrogenase) (MMSDH) Rv0753c, (MTV041.27c), len: 510 aa. Probable mmsA, methylmalonic acid semialdehyde dehydrogenase, highly similar to others e.g. NP_420115.1|NC_002696 putative methylmalonate-semialdehyde dehydrogenase from Caulobacter crescentus (499 aa); L48550|STMMSDA_1|CAB75315.1|AL139164 methylmalonic acid semialdehyde dehydrogenase from Streptomyces coelicolor (500 aa), FASTA score: (51.6% identity in 498 aa overlap); M84911|PSEMMSRAB_2|NP_252260.1|NC_002516 methylmalonate-semialdehyde dehydrogenase from Pseudomonas aeruginosa (497 aa), FASTA scores: opt: 1127, E(): 0, (47.9% identity in 507 aa overlap); etc. Note that also highly similar to malonic semialdehyde oxidative decarboxylases e.g. NP_104968.1|NC_002678 malonic semialdehyde oxidative decarboxylase from Mesorhizobium loti (498 aa); NP_384832.1|NC_003047 putative malonic semialdehyde oxidative decarboxylase protein from Sinorhizobium meliloti (498 aa); etc. Contains PS00070 Aldehyde dehydrogenases cysteine active site. Belongs to the aldehyde dehydrogenases family. O53816 No intermediary metabolism and respiration MMAR_1079 MSMEG_1498 Mb0775c NC_000962.3 Mycobrowser_v2 RBS 845959 845962 . - 0 Rv0753c mmsA Plays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA [catalytic activity: 2-methyl-3-oxopropanoate + CoA + NAD+ = propanoyl-CoA + CO2 + NADH]. Probable methylmalonate-semialdehyde dehydrogenase MmsA (methylmalonic acid semialdehyde dehydrogenase) (MMSDH) Rv0753c, (MTV041.27c), len: 510 aa. Probable mmsA, methylmalonic acid semialdehyde dehydrogenase, highly similar to others e.g. NP_420115.1|NC_002696 putative methylmalonate-semialdehyde dehydrogenase from Caulobacter crescentus (499 aa); L48550|STMMSDA_1|CAB75315.1|AL139164 methylmalonic acid semialdehyde dehydrogenase from Streptomyces coelicolor (500 aa), FASTA score: (51.6% identity in 498 aa overlap); M84911|PSEMMSRAB_2|NP_252260.1|NC_002516 methylmalonate-semialdehyde dehydrogenase from Pseudomonas aeruginosa (497 aa), FASTA scores: opt: 1127, E(): 0, (47.9% identity in 507 aa overlap); etc. Note that also highly similar to malonic semialdehyde oxidative decarboxylases e.g. NP_104968.1|NC_002678 malonic semialdehyde oxidative decarboxylase from Mesorhizobium loti (498 aa); NP_384832.1|NC_003047 putative malonic semialdehyde oxidative decarboxylase protein from Sinorhizobium meliloti (498 aa); etc. Contains PS00070 Aldehyde dehydrogenases cysteine active site. Belongs to the aldehyde dehydrogenases family. O53816 No intermediary metabolism and respiration MMAR_1079 MSMEG_1498 Mb0775c NC_000962.3 Mycobrowser_v2 RBS 846148 846152 . + 0 Rv0754 PE_PGRS11 Function unknown PE-PGRS family protein PE_PGRS11 Rv0754, (MTV041.28), len: 584 aa. PE_PGRS11, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), similar to others e.g. AL0212|MTV008_46 from Mycobacterium tuberculosis (1660 aa), FASTA score: (48.7% identity in 345 aa overlap); Z80225|MTCY441_4 from Mycobacterium tuberculosis (778 aa), FASTA score: (41.6% identity in 442 aa overlap); etc. Q79FW5 No PE/PPE ML2241 MMAR_4953 Mb0776 NC_000962.3 Mycobrowser_v2 CDS 846159 847913 . + 0 Rv0754 PE_PGRS11 Function unknown PE-PGRS family protein PE_PGRS11 Rv0754, (MTV041.28), len: 584 aa. PE_PGRS11, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), similar to others e.g. AL0212|MTV008_46 from Mycobacterium tuberculosis (1660 aa), FASTA score: (48.7% identity in 345 aa overlap); Z80225|MTCY441_4 from Mycobacterium tuberculosis (778 aa), FASTA score: (41.6% identity in 442 aa overlap); etc. Q79FW5 No PE/PPE ML2241 MMAR_4953 Mb0776 NC_000962.3 Mycobrowser_v2 CDS 848103 850040 . - 0 Rv0755c PPE12 Function unknown PPE family protein PPE12 Rv0755c, (MTV041.29), len: 645 aa. PPE12, Member of the Mycobacterium tuberculosis PPE family, highly similar to others e.g. Z82098|MTCY3C7_23 from Mycobacterium tuberculosis (582 aa), FASTA scores: (56.1% identity in 636 aa overlap); Z92774|MTCY6G11_5 from Mycobacterium tuberculosis (552 aa), FASTA scores: (55.8% identity in 590 aa overlap); etc. Predicted to be an outer membrane protein (See Song et al., 2008). Q79FW4 No PE/PPE Mb0777c NC_000962.3 Mycobrowser_v2 RBS 850047 850051 . - 0 Rv0755c PPE12 Function unknown PPE family protein PPE12 Rv0755c, (MTV041.29), len: 645 aa. PPE12, Member of the Mycobacterium tuberculosis PPE family, highly similar to others e.g. Z82098|MTCY3C7_23 from Mycobacterium tuberculosis (582 aa), FASTA scores: (56.1% identity in 636 aa overlap); Z92774|MTCY6G11_5 from Mycobacterium tuberculosis (552 aa), FASTA scores: (55.8% identity in 590 aa overlap); etc. Predicted to be an outer membrane protein (See Song et al., 2008). Q79FW4 No PE/PPE Mb0777c NC_000962.3 Mycobrowser_v2 CDS 850342 850527 . - 0 Rv0755A Rv0755A Could be required for the transposition of an insertion element. Putative transposase (fragment) Rv0755A, len: 61 aa. Putative transposase (possibly gene fragment), similar to C-terminal part of Q9EZM2|ISMav2|AF286339_1 putative transposase from Mycobacterium paratuberculosis (395 aa), FASTA scores: opt: 284, E(): 5e-13, (83.02% identity in 53 aa overlap); and to SCJ11.25c|Q9RI80 possible noncomposite transposon transposase from Streptomyces coelicolor (283 aa). Q79FW3 No insertion seqs and phages Mb0778c NC_000962.3 Mycobrowser_v2 CDS 851608 852351 . + 0 Rv0757 phoP Involved in transcriptional mechanism. Part of the two component regulatory system PHOP/PHOQ. This protein is thought to be a positive regulator for the phosphate regulon, required for intracellular growth. Transcription of this operon is positively regulated by PHOB and PHOR|Rv0758 when phosphate is limited. Possible two component system response transcriptional positive regulator PhoP Rv0757, (MTCY369.02), len: 247 aa. Possible phoP, two component system response phosphate regulon transcriptional regulator (see citations below), highly similar to various transcriptional regulators e.g. CAC32360.1|AL583945 putative two component system response regulator from Streptomyces coelicolor (271 aa); T45446 probable two-component response regulator from Mycobacterium leprae (253 aa); and similar to phoP proteins e.g. P13792|PHOP_BACSU alkaline phosphatase synthesis transcription regulatory protein from Bacillus subtilis (240 aa), FASTA scores: opt: 594, E(): 2.3e-33, (41.0% identity in 234 aa overlap); etc. Also highly similar to Rv3765c from Mycobacterium tuberculosis (234 aa), Rv1033c (257 aa), RV0903c|MTCY31.31c|Q10531 (236 aa), FASTA score: (45.4% identity in 229 aa overlap); MTCY10G2_16 and MTU88959_1. P71814 No regulatory proteins ML2239 MMAR_4942 MSMEG_5872 Mb0780 NC_000962.3 Mycobrowser_v2 CDS 852396 853853 . + 0 Rv0758 phoR Sensor part of a two component regulatory system. This protein is thought to be a sensor kinase for the phosphate regulon. Transcription of this operon is positively regulated by PHOB|Rv0757 and PHOR when phosphate is limited. Possible two component system response sensor kinase membrane associated PhoR Rv0758, (MTCY369.03), len: 485 aa. Possible phoR, two component system response phosphate sensor kinase membrane-associated, highly similar to various sensor kinases e.g. CAC32361.1|AL583945 putative two component system histidine kinase from Streptomyces coelicolor (524 aa); NP_349365.1|NC_003030 Membrane-associated sensory histidine kinase with HAMP domain from Clostridium acetobutylicum (482 aa); and similar to phoP proteins e.g. NP_372216.1|NC_002758 alkaline phosphatase synthesis sensor protein from Staphylococcus aureus (554 aa); P23545|PHOR_BACSU alkaline phosphatase synthesis sensor from Bacillus subtilis (579 aa), FASTA scores: opt: 515, E(): 1.9e-25, (40.0% identity in 230 aa overlap); etc. Also similar to proteins from Mycobacterium tuberculosis e.g. MTCY20G9.16 FASTA scores: (34.5% identity in 264 aa overlap), MTU88959_2 (509 aa), MTCY10G2_17, etc. P71815 No regulatory proteins ML2238 MMAR_4941 MSMEG_5870 Mb0781 NC_000962.3 Mycobrowser_v2 CDS 1243010 1243504 . - 0 Rv1120c Rv1120c Function unknown Conserved hypothetical protein Rv1120c, (MTCY22G8.09c), len: 164 aa. Conserved hypothetical protein, some similarity at C-terminus to Mycobacterium tuberculosis hypothetical proteins e.g. Rv1890c|MTCY180.28 (462 aa), FASTA scores: opt: 187, E(): 2.2e-05, (36.6% identity in 93 aa overlap) and Rv2488c|YZ19_MYCTU|Q10551 (285 aa), FASTA scores: opt: 156, E(): 0.00074, (32.7% identity in 107 aa overlap). O06572 No conserved hypotheticals Mb1151c NC_000962.3 Mycobrowser_v2 CDS 853825 854157 . - 0 Rv0759c Rv0759c Function unknown Conserved hypothetical protein Rv0759c, (MTCY369.04c), len: 110 aa. Conserved hypothetical protein, highly similar (but shorter 45 aa in N-terminus) to P49774|YHIT_MYCLE|ML2237|MLCB5.04c|U296A hypothetical hit-like protein from Mycobacterium leprae (155 aa), FASTA scores: opt: 766, E(): 0, (78.7% identity in 150 aa overlap). Also highly similar (but N-terminus always shorter) to hit-like proteins and protein kinase inhibitors e.g. AAF72728.1|AF265258_1|AF265258 hit-like protein from Rhodococcus sp. (141 aa); NP_212513.1|NC_001318 protein kinase C1 inhibitor (pkcI) from Borrelia burgdorferi (149 aa) ; P94252|YHIT_BORBU|BB0379 hypothetical hit-like protein from Borrelia burgdorferi (139 aa); NP_110768.1|NC_002689 hit (histidine triad) family protein from Thermoplasma volcanium (158 aa); P16436|IPK1_BOVIN protein kinase C inhibitor 1 (pkci-1) from Bos taurus (Bovine) (125 aa), FASTA scores: opt: 195, E(): 5.2e-08, (33.3% identity in 111 aa overlap); etc. Also shows similarity with Rv2613c|MTCY01A10.20A conserved hypothetical protein from Mycobacterium tuberculosis (195 aa) and Rv1262c|MTCY50.20 hypothetical hit-like protein (144 aa). P0A5B5 No conserved hypotheticals ML2237 MMAR_4940 MSMEG_5871 Mb0782c NC_000962.3 Mycobrowser_v2 CDS 854267 854686 . - 0 Rv0760c Rv0760c Function unknown Conserved protein Rv0760c, (MTCY369.05), len: 139 aa. Conserved protein, similar to N-terminal part of Rv2042c conserved hypothetical protein from Mycobacterium tuberculosis (265 aa), FASTA scores: opt: 150, E(): 4.1e-05, (28.7% identity in 136 aa overlap). Belongs to the NTF2-like (nuclear transport factor 2) protein superfamiily. P71817 No conserved hypotheticals MMAR_4937 MSMEG_5867 Mb0783c NC_000962.3 Mycobrowser_v2 CDS 854699 855826 . - 0 Rv0761c adhB Thought to catalyze the reversible oxidation of ethanol to acetaldehyde with the concomitant reduction of NAD. Probably acts on primary or secondary alcohols or hemiacetals [catalytic activity: an alcohol + NAD+ = an aldehyde or ketone + NADH]. Possible zinc-containing alcohol dehydrogenase NAD dependent AdhB Rv0761c, (MTCY369.06c), len: 375 aa. Possible adhB, zinc-containing alcohol dehydrogenase NAD-dependent, similar to others e.g. AAC15839.1|AF060871_4 hypothetical alcohol dehydrogenase from Rhodococcus rhodochrous (370 aa), FASTA scores: opt: 1234, E(): 0, (46.8% identity in 370 aa overlap); P80468|ADH2_STRCA alcohol dehydrogenase II from Struthio camelus (Ostrich) (379 aa); Q03505|ADH1_RABIT alcohol dehydrogenase alpha chain from Oryctolagus cuniculus (Rabbit) (374 aa), FASTA scores: opt: 872, E(): 0, (39.1% identity in 379 aa overlap); etc. Also similar to adhD alcohol dehydrogenase from Mycobacterium tuberculosis (368 aa). Contains PS00059 Zinc-containing alcohol dehydrogenases signature. Belongs to the zinc-containing alcohol dehydrogenase family. P71818 No intermediary metabolism and respiration MMAR_4935 MSMEG_5866 Mb0784c NC_000962.3 Mycobrowser_v2 CDS 855925 856470 . - 0 Rv0762c Rv0762c Function unknown Conserved hypothetical protein Rv0762c, (MTCY369.07c), len: 181 aa. Conserved hypothetical protein, showing weak similarity to D90907_77|P73575 hypothetical 31.3KD protein from Synechocystis sp, FASTA scores: E(): 0.0012, (30.4% identity in 92 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). P71819 No conserved hypotheticals MMAR_4934 MSMEG_5865 Mb0785c NC_000962.3 Mycobrowser_v2 CDS 856473 856679 . - 0 Rv0763c Rv0763c Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Probably involved in electron transport for cytochrome P-450 system. Possible ferredoxin Rv0763c, (MTCY369.08c), len: 68 aa. Possible ferredoxin, similar to others and related proteins e.g. P18324|FER1_STRGO|SUAB ferredoxin 1 (fd-1) from Streptomyces griseolus (68 aa); AAK31349.1|AF350429_2|AF350429 putative ferredoxin from Nocardioides sp (63 aa); AAK16536.1|AF331043_16|AF331043 phthalate dioxygenase ferredoxin subunit from Arthrobacter keyseri (64 aa); etc. Probably involved in electron transport for cytochrome P-450 system e.g. downstream ORF Rv0764c|MTCY369.09c probable cytochrome P450 51 from Mycobacterium tuberculosis (451 aa), FASTA scores: opt: 137, E(): 0.00013, (36.4% identity in 66 aa overlap). Also similar to putative ferredoxins Rv3503c and Rv1786 from Mycobacterium tuberculosis. Could belong to the bacterial type ferredoxin family. P71820 No intermediary metabolism and respiration MSMEG_5864 Mb0786c NC_000962.3 Mycobrowser_v2 CDS 1419961 1420302 . - 0 Rv1271c Rv1271c Unknown Conserved hypothetical secreted protein Rv1271c, (MTCY50.11), len: 113 aa. Conserved hypothetical exported protein with potential N-terminal signal sequence. Similar to Mycobacterium tuberculosis hypothetical proteins Rv1804c, Rv1810, Rv0622, etc. P64793 No cell wall and cell processes MMAR_4150,MMAR_4151 Mb1302c NC_000962.3 Mycobrowser_v2 CDS 856682 858037 . - 0 Rv0764c cyp51 Involved in sterol biosynthesis. Its precise biological substrate is not known. Catalyzes C14-demethylation of lanosterol, 24,25-dihydrolanosterol and obtusifoliol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol. Cytochrome P450 51 Cyp51 (CYPL1) (P450-L1A1) (sterol 14-alpha demethylase) (lanosterol 14-alpha demethylase) (P450-14DM) Rv0764c, (MT0788, MTCY369.09c), len: 451 aa. Cyp51, cytochrome P450 51 (sterol 14-alpha demethylase), similar to others e.g. Q16850|CP51_HUMAN cytochrome P450 51 (CYPL1) (P450L1) (sterol 14-alpha demethylase) (lanosterol 14-alpha demethylase) from Homo sapiens (509 aa), FASTA scores: opt: 848, E(): 0, (33.9% identity in 439 aa overlap); NP_172633.1|NC_003070 putative obtusifoliol 14-alpha demethylase from Arabidopsis thaliana (488 aa); P93596|CP51_WHEAT cytochrome P450 51 (CYPL1) (P450-L1A1) (obtusifoliol 14-alpha demethylase) from Triticum aestivum (453 aa); etc. Also similar to many other Mycobacterium tuberculosis cytochromes P450 e.g. Rv1394c, FASTA score: (22.5% identity in 444 aa overlap). Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. Belongs to the cytochrome P450 family. P0A512 No intermediary metabolism and respiration MMAR_4932 MSMEG_5863 Mb0787c NC_000962.3 Mycobrowser_v2 CDS 858037 858864 . - 0 Rv0765c Rv0765c Function unknown; probably involved in cellular metabolism, possibly electron transfer. Probable oxidoreductase Rv0765c, (MTCY369.10c), len: 275 aa. Probable oxidoreductase, similar others e.g. P39071|DHBA_BACSU 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase from Bacillus subtilis (261 aa), FASTA scores: opt: 385, E(): 1.8e-17, (30.6% identity in 252 aa overlap); AAF81239.1|AF263012 putative beta-ketoacyl reductase from Streptomyces griseus (274 aa); NP_436514.1|NC_003037 putative oxidoreductase from Sinorhizobium meliloti (240 aa); etc. Also similar to several other oxidoreductases from Mycobacterium tuberculosis e.g. Rv1544|MTCY48.21, FASTA score: (32.6% identity in 267 aa overlap); etc. Contains PS00061 Short-chain alcohol dehydrogenase family signature. P71821 No intermediary metabolism and respiration MMAR_4931 MSMEG_5862 Mb0788c NC_000962.3 Mycobrowser_v2 CDS 858864 860072 . - 0 Rv0766c cyp123 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Probable cytochrome P450 123 Cyp123 Rv0766c, (MT0790, MTCY369.11c), len: 402 aa. Probable cyp123, cytochrome P-450, similar to others e.g. P33271|CPXK_SACER cytochrome P-450 107B1 from Saccharopolyspora erythraea (405 aa), FASTA scores: opt: 770, E(): 0, (36.9% identity in 406 aa overlap); T36526 probable cytochrome P450 hydroxylase from Streptomyces coelicolor (411 aa); P27632|CPXM_BACSU cytochrome P450 109 from Bacillus subtilis (405 aa); etc. Also similar to several other cytochromes P-450 from Mycobacterium tuberculosis e.g. Rv1256c|MTCY50.26 (405 aa), FASTA score: (35.2% identity in 389 aa overlap); etc. Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. Belongs to the cytochrome P450 family. P63707 No intermediary metabolism and respiration MMAR_4930 MSMEG_5861 Mb0789c NC_000962.3 Mycobrowser_v2 CDS 860069 860710 . - 0 Rv0767c Rv0767c Function unknown Conserved hypothetical protein Rv0767c, (MTCY369.12c), len: 213 aa. Conserved hypothetical protein, showing weak similarity with AL133220|SCC75A_26 hypothetical protein from Streptomyces coelicolor (215 aa), FASTA scores: opt: 152, E(): 0.0048, (28.4% identity in 204 aa overlap). P67432 No conserved hypotheticals MMAR_4929 MSMEG_5860 Mb0790c NC_000962.3 Mycobrowser_v2 CDS 860912 862381 . + 0 Rv0768 aldA Oxidizes a variety of aldehydes [catalytic activity: an aldehyde + NAD+ + H2O = an acid + NADH]. Probable aldehyde dehydrogenase NAD dependent AldA (aldehyde dehydrogenase [NAD+]) Rv0768, (MTCY369.13), len: 489 aa. Probable aldA, NAD-dependent aldehyde dehydrogenase, highly similar to others e.g. AAL14238.1|AY052630 6-oxolauric acid dehydrogenase from Rhodococcus ruber (474 aa); NP_285450.1|NC_001264 aldehyde dehydrogenase from Deinococcus radiodurans (495 aa); NP_241405.1|NC_002570 NADP-dependent aldehyde dehydrogenase from Bacillus halodurans (498 aa); P42757|DHAB_ATRHO betaine-aldehyde dehydrogenase precursor from Atriplex hortensis (Mountain spinach) (502 aa), FASTA scores: opt: 1001, E(): 0, (35.6% identity in 486 aa overlap); etc. Also highly similar to Rv0223c aldehyde dehydrogenase from Mycobacterium tuberculosis (487 aa). Contains PS00687 Aldehyde dehydrogenases glutamic acid active site. Belongs to the aldehyde dehydrogenases family. P71823 No intermediary metabolism and respiration MMAR_4928 MSMEG_5859 Mb0791 NC_000962.3 Mycobrowser_v2 RBS 3086809 3086812 . + 0 Rv0768 aldA Oxidizes a variety of aldehydes [catalytic activity: an aldehyde + NAD+ + H2O = an acid + NADH]. Probable aldehyde dehydrogenase NAD dependent AldA (aldehyde dehydrogenase [NAD+]) Rv0768, (MTCY369.13), len: 489 aa. Probable aldA, NAD-dependent aldehyde dehydrogenase, highly similar to others e.g. AAL14238.1|AY052630 6-oxolauric acid dehydrogenase from Rhodococcus ruber (474 aa); NP_285450.1|NC_001264 aldehyde dehydrogenase from Deinococcus radiodurans (495 aa); NP_241405.1|NC_002570 NADP-dependent aldehyde dehydrogenase from Bacillus halodurans (498 aa); P42757|DHAB_ATRHO betaine-aldehyde dehydrogenase precursor from Atriplex hortensis (Mountain spinach) (502 aa), FASTA scores: opt: 1001, E(): 0, (35.6% identity in 486 aa overlap); etc. Also highly similar to Rv0223c aldehyde dehydrogenase from Mycobacterium tuberculosis (487 aa). Contains PS00687 Aldehyde dehydrogenases glutamic acid active site. Belongs to the aldehyde dehydrogenases family. P71823 No intermediary metabolism and respiration MMAR_4928 MSMEG_5859 Mb0791 NC_000962.3 Mycobrowser_v2 CDS 862412 863158 . + 0 Rv0769 Rv0769 Function unknown; probably involved in cellular metabolism. Probable dehydrogenase/reductase Rv0769, (MTCY369.14), len: 248 aa. Probable dehydrogenase/reductase, similar to others, especially short-chain type dehydrogenases/reductases and 3-oxoacyl-(acyl-carrier protein) reductases e.g. NP_106890.1|NC_002678 probable short-chain type dehydrogenase/reductase from Mesorhizobium loti (374 aa); NP_243357.1|NC_002570 3-oxoacyl-(acyl-carrier protein) reductase from Bacillus halodurans (246 aa); P28643|FABG_CUPLA 3-oxoacyl-[acyl-carrier protein] reductase from Cuphea lanceolata (320 aa); P25529|HDHA_ECOLI 7-alpha-hydroxysteroid dehydrogenase from Escherichia coli (255 aa), FASTA scores: opt: 536, E(): 6.5e-27, (37.7% identity in 247 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. MTCY02B10.14, FASTA score: (33.7% identity in 249 aa overlap); etc. P71824 No intermediary metabolism and respiration MMAR_4927 MSMEG_5858 Mb0792 NC_000962.3 Mycobrowser_v2 CDS 863256 864143 . + 0 Rv0770 Rv0770 Function unknown; 3-hydroxyisobutyrate dehydrogenase family protein probably involved in cellular metabolism. Probable dehydrogenase/reductase Rv0770, (MTCY369.15), len: 295 aa. Probable dehydrogenase/reductase, 3-hydroxyisobutyrate dehydrogenase family, possibly 3-hydroxyisobutyrate dehydrogenase or 2-hydroxy-3-oxopropionate reductase, similar to others e.g. P23523|GARR_ECOLI 2-hydroxy-3-oxopropionate reductase (tartronate semialdehyde reductase) (TSAR) from Escherichia coli strain K12 (294 aa), FASTA scores: opt: 469, E(): 6.7e-22, (34.4% identity in 282 aa overlap); P28811|MMSB_PSEAE 3-hydroxyisobutyrate dehydrogenase (hibadh) from Pseudomonas aeruginosa (298 aa), FASTA scores: opt: 439, E(): 4.3e-20, (34.9% identity in 269 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. Rv1122 and Rv1844c. Seems to belong to the 3-hydroxyisobutyrate dehydrogenase family. P71825 No intermediary metabolism and respiration MMAR_4926 MSMEG_5857 Mb0793 NC_000962.3 Mycobrowser_v2 CDS 864140 864574 . + 0 Rv0771 Rv0771 Involved in aromatic hydrocarbons catabolism. Thought to be involved in the catabolism of protocatechuate to succinate-and acetyl-CoA in the beta-ketoadipate pathway (at the third step) [catalytic activity: 2-carboxy-5-oxo-2,5-dihydrofuran-2-acetate = 5-oxo-4,5-dihydrofuran-2-acetate + CO(2)]. Possible 4-carboxymuconolactone decarboxylase (CMD) Rv0771, (MTCY369.16), len: 144 aa. Possible 4-carboxymuconolactone decarboxylase, showing similarity with other carboxymuconolactone decarboxylases e.g. AAD39557.1|AF031417 PcaC-like protein from Pseudomonas putida (130 aa); P20370|DC4C_ACICA 4-carboxymuconolactone decarboxylase (CMD) from Acinetobacter sp. ADP1 (134 aa), FASTA scores: opt: 174, E(): 0.00075, (31.4% identity in 121 aa overlap); C-terminus of NP_421214.1|NC_002696 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase from Caulobacter crescentus (393 aa); C-terminus of T47115 probable 4-carboxymuconolactone decarboxylase / 3-oxoadipate enol-lactone hydrolase from Streptomyces sp (373 aa); NP_407104.1|NC_003143 putative gamma carboxymuconolactone decarboxylase from Yersinia pestis (131 aa); etc. P71826 No intermediary metabolism and respiration MMAR_4925 MSMEG_5856 Mb0794 NC_000962.3 Mycobrowser_v2 CDS 864586 865854 . + 0 Rv0772 purD Involved in de novo purine biosynthesis (at the second step) [catalytic activity: ATP + 5-phosphoribosylamine + glycine = ADP + phosphate + 5'-phosphoribosylglycinamide]. Probable phosphoribosylamine--glycine ligase PurD (GARS) (glycinamide ribonucleotide synthetase) (phosphoribosylglycinamide synthetase) (5'-phosphoribosylglycinamide synthetase) Rv0772, (MTCY369.17), len: 422 aa. Probable purD, phosphoribosylamine--glycine ligase, equivalent to Q50144|PURD|PUR2_MYCLE|ML2235|MLCB5.08 phosphoribosylamine--glycine ligase from Mycobacterium leprae (422 aa), FASTA scores: opt: 2272, E(): 0, (81.8% identity in 422 aa overlap). Also highly similar to others e.g. CAB56348.1|AL118514 phosphoribosylamine-glycine ligase from Streptomyces coelicolor (416 aa); P1564|PUR2_ECOLI phosphoribosylamine--glycine ligase from Escherichia coli (429 aa), FASTA scores: opt: 1039, E(): 0, (42.7% identity in 431 aa overlap); etc. Belongs to the GarS family. P65893 No intermediary metabolism and respiration ML2235 MMAR_4924 MSMEG_5852 Mb0795 NC_000962.3 Mycobrowser_v2 CDS 2330214 2330963 . - 0 Rv2073c Rv2073c Unknown Probable shortchain dehydrogenase Rv2073c, (MTCY49.12c), len: 249 aa. Probable oxidoreductase, belonging to shortchain dehydrogenase reductase (SDR) family, similar to many. Q10681 No intermediary metabolism and respiration MMAR_3055 MSMEG_3879 NC_000962.3 Mycobrowser_v2 CDS 865851 867389 . - 0 Rv0773c ggtA Besides the cephalosporin acylase I activity which converts GL-7ACA into 7-ACA; this enzyme displays some gamma glutamyltranspeptidase activity: GGT plays a key role in the gamma-glutamyl cycle, a pathway for the synthesis and degradation of glutathione. [catalytic activity 1: 7-beta-(4-carboxybutanamido)-cephalosporanic acid + H2O = 7-aminocephalosporanic acid + glutaric acid] [catalytic activity 2: (5-L-glutamyl)-peptide + an amino acid = peptide + 5-L-glutamyl-amino acid]. Probable bifunctional acylase GgtA: cephalosporin acylase (GL-7ACA acylase) + gamma-glutamyltranspeptidase (GGT) Rv0773c, (MTCY369.18), len: 512 aa. Probable ggtA, bifunctional acylase including cephalosporin acylase, and gamma-glutamyl transpeptidase; highly similar to others e.g. NP_295247.1|NC_001263 cephalosporin acylase from Deinococcus radiodurans (535 aa); NP_248854.1|NC_002516 probable gamma-glutamyltranspeptidase from Pseudomonas aeruginosa (538 aa); P15557|PAC1_PSES3 acylase ACY 1 [includes: cephalosporin acylase (GL-7ACA acylase); gamma-glutamyltranspeptidase (GGT)] from Pseudomonas sp. strain SE83 (558 aa), FASTA scores: opt: 784, E(): 0, (34.2% identity in 526 aa overlap); NP_391491.1|NC_000964|Z93767|BSZ93767_6|O0521 protein similar to gamma-glutamyltransferase from Bacillus subtilis (525 aa), FASTA scores: opt: 1169, E(): 0, (40.1% identity in 516 aa overlap); etc. Also similar to Rv2394|ggtB from Mycobacterium tuberculosis. Member of GL-7ACA acylases and to GGT group. P71828 No intermediary metabolism and respiration ML2234 MMAR_4920 MSMEG_1463 Mb0796c NC_000962.3 Mycobrowser_v2 CDS 867440 868351 . - 0 Rv0774c Rv0774c Unknown; could have possibly a lipolytic activity. Probable conserved exported protein Rv0774c, (MTCY369.19c), len: 303 aa. Possible conserved exported protein with hydrophobic region near N-terminus, highly similar, except in N-terminus, to Rv0519c|Z97831|MTY20G10.09c|O33364 hypothetical protein from Mycobacterium tuberculosis (300 aa), FASTA scores: opt: 1092, E(): 0, (57.9% identity in 299 aa overlap). Contains PS00061 Short-chain alcohol dehydrogenase family signature, and PS00120 Lipases, serine active site. So could be a lipase. Start changed since first submission (-9 aa). Predicted to be an outer membrane protein (See Song et al., 2008). P71829 No cell wall and cell processes ML2233 MMAR_4919 MSMEG_5851 Mb0797c NC_000962.3 Mycobrowser_v2 CDS 868407 869030 . + 0 Rv0775 Rv0775 Function unknown Conserved hypothetical protein Rv0775, (MTCY369.20), len: 207 aa. Conserved hypothetical protein, showing some similarity to other proteins e.g. ECAE000186_11|MG1655 hypothetical protein from Escherichia coli strain K-12 (178 aa), FASTA scores: E(): 6.4e-05, (27.2% identity in 147 aa overlap); P41037|BIH_ECOLI hypothetical transcriptional regulator from Escherichia coli (103 aa), FASTA scores: opt: 138, E(): 0.003, (30.9% identity in 97 aa overlap); etc. P71830 No conserved hypotheticals ML2232 MMAR_4918 MSMEG_5850 Mb0798 NC_000962.3 Mycobrowser_v2 CDS 868984 869763 . - 0 Rv0776c Rv0776c Function unknown Conserved hypothetical protein Rv0776c, (MTCY369.21a), len: 259 aa. Conserved hypothetical protein, similar (except first 50 aa) to P72737|D90900_57 hypothetical protein from Synechocystis sp. strain PCC 6803 (261 aa), FASTA scores: opt: 337, E(): 1.7e-15, (30.5% identity in 266 aa overlap). P71831 No conserved hypotheticals ML2231 MMAR_4917 Mb0799c NC_000962.3 Mycobrowser_v2 CDS 878638 879324 . + 0 Rv0784 Rv0784 Function unknown Conserved hypothetical protein Rv0784, (MTC369.28), len: 228 aa. Conserved hypothetical protein, with some similarity to MLCB5_20|O05752 hypothetical protein from Mycobacterium leprae (193 aa), FASTA scores: opt: 141, E(): 0.0022, (36.0% identity in 114 aa overlap). Also similar to N-terminus of NP_253002.1|NC_002516 conserved hypothetical protein from Pseudomonas aeruginosa (253 aa). P71837 No conserved hypotheticals ML2223 MMAR_4904 MSMEG_5836 Mb0806 NC_000962.3 Mycobrowser_v2 RBS 910964 910967 . + 0 Rv0818 Rv0818 Involved in transcriptional mechanism. Transcriptional regulatory protein Rv0818, (MTV043.10), len: 255 aa. Probable transcriptional regulatory protein, highly similar to Q05943|GLNR_STRCO|L03213|STMGLNR_1|SCD84.26c transcriptional regulatory protein from Streptomyces coelicolor (267 aa), FASTA scores: opt: 945, E(): 0, (61.5 identity in 239 aa overlap); and similar to others from other organisms. Also similar to Rv2884|MTCY274.15|Z74024 from Mycobacterium tuberculosis (252 aa), FASTA scores: opt: 662, E(): 0, (47.8% identity in 226 aa overlap). O53830 No regulatory proteins ML2194 MMAR_4865 MSMEG_5784 Mb0841 NC_000962.3 Mycobrowser_v2 CDS 910972 911739 . + 0 Rv0818 Rv0818 Involved in transcriptional mechanism. Transcriptional regulatory protein Rv0818, (MTV043.10), len: 255 aa. Probable transcriptional regulatory protein, highly similar to Q05943|GLNR_STRCO|L03213|STMGLNR_1|SCD84.26c transcriptional regulatory protein from Streptomyces coelicolor (267 aa), FASTA scores: opt: 945, E(): 0, (61.5 identity in 239 aa overlap); and similar to others from other organisms. Also similar to Rv2884|MTCY274.15|Z74024 from Mycobacterium tuberculosis (252 aa), FASTA scores: opt: 662, E(): 0, (47.8% identity in 226 aa overlap). O53830 No regulatory proteins ML2194 MMAR_4865 MSMEG_5784 Mb0841 NC_000962.3 Mycobrowser_v2 CDS 870008 871426 . + 0 Rv0777 purB Involved in de novo purine biosynthesis (at the eight step) [catalytic activity: 1-(5-phosphoribosyl)-4-(N-succino-carboxamide) -5-aminoimidazole = fumarate + 5'-phosphoribosyl-5-amino-4-imidazolecarboxamide (also catalyzes: N6-(1,2-dicarboxyethyl)AMP = fumarate + AMP)]. Probable adenylosuccinate lyase PurB (adenylosuccinase) (ASL) (ASASE) Rv0777, (MTCY369.21b), len: 472 aa. Probable purB, adenylosuccinate lyase, equivalent (but shorter 15 aa) to MLCB5.13|Z95151|g2076607|PURB adenylosuccinate lyase from Mycobacterium leprae (487 aa), FASTA scores: opt: 2640, E(): 0, (86.7% identity in 472 aa overlap). More similar to eukaryotic adenylosuccinate lyases than to prokaryotic adenylosuccinate lyases e.g. P54822|PUR8_MOUSE adenylosuccinate lyase from Mus musculus (484 aa), FASTA scores: opt: 762, E(): 0, (32.4% identity in 445 aa overlap); CAB99134.1|AL390188 putative adenylosuccino lyase (fragment) from Streptomyces coelicolor (362 aa); etc. Contains PS00163 Fumarate lyases signature. Belongs to the lyase 1 family, adenylossucinate lyase subfamily. P71832 No intermediary metabolism and respiration ML2230 MMAR_4916 MSMEG_5847 Mb0800 NC_000962.3 Mycobrowser_v2 CDS 871431 872675 . + 0 Rv0778 cyp126 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Possible cytochrome P450 126 Cyp126 Rv0778, (MT0802, MTCY369.22), len: 414 aa. Possible cyp126, cytochrome P-450, similar to other cytochromes and related proteins e.g. AAG29781.1|AF235050_4|AF235050 cytochrome P-450 from Streptomyces rishiriensis (407 aa); Q59723|PSECYTOCHR_1 cytochrome p-450 linalool 8-monooxygenase (lin C) from Pseudomonas incognita (406 aa), FASTA scores: opt: 769, E(): 0, (37.0% identity in 411 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. Rv0766c, Rv2266, Rv3545c, etc. Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. P63711 No intermediary metabolism and respiration ML2229 MMAR_4915 MSMEG_5846 Mb0801 NC_000962.3 Mycobrowser_v2 CDS 872672 873292 . - 0 Rv0779c Rv0779c Unknown Possible conserved transmembrane protein Rv0779c, (MTCY369.23c), len: 206 aa. Possible conserved transmembrane protein, equivalent to Z95151|MLCB5_14 O05747 conserved hypothetical protein from Mycobacterium leprae (206 aa), FASTA scores: opt: 902, E(): 0, (67.2% identity in 204 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P71833 No cell wall and cell processes ML2228 MSMEG_5842 Mb0802c NC_000962.3 Mycobrowser_v2 CDS 873343 874236 . + 0 Rv0780 purC Involved in de novo purine biosynthesis (at the seventh step) [catalytic activity: ATP + 1-(5-phosphoribosyl)-4-carboxy-5-aminoimidazole + L-aspartate = ADP + phosphate + 1-(5-phosphoribosyl)-4-(N-succino-carboxamide)-5-aminoimidazole]. Phosphoribosylaminoimidazole- succinocarboxamide synthase PurC (SAICAR synthetase) Rv0780, (MTCY369.24), len: 297 aa. PurC, phosphoribosylaminoimidazole- succinocarboxamide synthase (see citations below), equivalent to MTU34957_1|PURC phosphoribosylaminoimidazole-succinocarboxamide synthase from Mycobacterium leprae (297 aa), FASTA scores: opt: 1986, E(): 0, (99.3% identity in 297 aa overlap). Also similar to others e.g. CAB56351.1|AL118514 phosphoribosylaminoimidazole-succinocarboxamide synthase from Streptomyces coelicolor (299 aa); etc. Contains PS01058 SAICAR synthetase signature 2. Belongs to the SAICAR synthetase family. P0A5T4 No intermediary metabolism and respiration ML2227 MMAR_4909 MSMEG_5841 Mb0803 NC_000962.3 Mycobrowser_v2 CDS 1029513 1030577 . - 0 Rv0923c Rv0923c Function unknown Conserved hypothetical protein Rv0923c, (MTCY21C12.17c), len: 354 aa. Conserved hypothetical protein, showing similarity with C-terminal part of AF034138|AF034138_7|yjoB hypothetical protein from Bacillus subtilis (200 aa), FASTA scores: opt: 193, E(): 4.2e-05, (32.3% identity in 167 aa overlap). O05915 No conserved hypotheticals MMAR_4586 MSMEG_5591 Mb0947c NC_000962.3 Mycobrowser_v2 CDS 882760 883434 . + 0 Rv0788 purQ Involved in de novo purine biosynthesis (at the fourth step) [catalytic activity: ATP + 5'-phosphoribosylformylglycinamide + L-glutamine + H2O = ADP + phosphate + 5'-phosphoribosylformylglycinamidine + L-glutamate]. Probable phosphoribosylformylglycinamidine synthase I PURG (FGAM synthase I) Rv0788, (MTCY369.32), len: 224 aa. Probable purQ, phosphoribosylformylglycinamidine synthase I, equivalent to MLCB5_24|Z95151|O05756|PURQ phosphoribosylformylglycinamidine synthase I from Mycobacterium leprae (224 aa), FASTA scores: opt: 1341, E(): 0, (88.7% identity in 222 aa overlap). Also highly similar to others e.g. P12041|PURQ_BACSU phosphoribosylformylglycinamidine synthase I from Bacillus subtilis (227 aa), FASTA scores: opt: 691, E(): 8.6e-39, (47.7% identity in 214 aa overlap); etc. Contains PS00442 Glutamine amidotransferases class-I active site. Belongs to type-1 glutamine amidotransferases. P65902 No intermediary metabolism and respiration ML2219 MMAR_4897 MSMEG_5831 Mb0812 NC_000962.3 Mycobrowser_v2 CDS 874233 874943 . + 0 Rv0781 ptrBa Cleaves peptide bonds on the C-terminal side of lysyl and argininyl residues [catalytic activity: hydrolysis of ARG-|-XAA and LYS-|-XAA bonds in oligopeptides, even when P1' residue is proline]. Probable protease II PtrBa [first part] (oligopeptidase B) Rv0781, (MTCY369.25), len: 236 aa. Probable ptrBa, first part of protease II, equivalent to N-terminus of NP_302455.1|NC_002677 protease II from Mycobacterium leprae (724 aa). Also highly similar to N-termini of many proteases II e.g. P24555|PTRB_ECOLI|TLP|B1845 protease II from Escherichia coli strains K12 and HB101 (707 aa), FASTA scores: opt: 204, E(): 7.4e-07, (29.6% identity in 230 aa overlap); etc. ORFs Rv0782 and Rv0781 appear to be a frameshifted homologues of protease II, but we can find no error in the cosmid sequence to account for this. Belongs to peptidase family S9A; also known as the prolyl oligopeptidase family. Note that previously known as ptrBb. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P71835 No intermediary metabolism and respiration Mb0804 NC_000962.3 Mycobrowser_v2 CDS 874732 876390 . + 0 Rv0782 ptrBb Cleaves peptide bonds on the C-terminal side of lysyl and argininyl residues [catalytic activity: hydrolysis of ARG-|-XAA and LYS-|-XAA bonds in oligopeptides, even when P1' residue is proline]. Probable protease II PtrBb [second part] (oligopeptidase B) Rv0782, (MTCY369.26), len: 552 aa. Probable ptrBb, second part of protease II, equivalent to C-terminus of NP_302455.1|NC_002677 protease II from Mycobacterium leprae (724 aa). Also highly similar to N-termini of many proteases II e.g. P24555|PTRB_ECOLI|TLP|B1845 protease II from Escherichia coli strains K12 and HB101 (707 aa), FASTA scores: opt: 1251, E(): 0, (42.7% identity in 489 aa overlap); etc. ORFs Rv0782 and Rv0781 appear to be a frameshifted homologues of protease II, but we can find no error in the cosmid sequence to account for this. Belongs to peptidase family S9A; also known as the prolyl oligopeptidase family. Note that previously known as ptrBa. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P71834 No intermediary metabolism and respiration ML2226 MMAR_4908 MSMEG_5839 Mb0804 NC_000962.3 Mycobrowser_v2 CDS 876818 878440 . - 0 Rv0783c emrB Translocase that confers resistance to substances of high hydrophobicity. Involved in transport of multidrug across the membrane (export): multidrug resistance by an export mechanism. Responsible for the translocation of the substrate across the membrane. Possible multidrug resistance integral membrane efflux protein EmrB Rv0783c, (MTCY369.27c), len: 540 aa. Possible emrB, integral membrane drug efflux protein, member of major facilitator superfamily (MFS), equivalent to AAL16083.1|AF421382_1|AF421382 EmrB efflux protein from Mycobacterium avium (538 aa). Also similar to other membrane proteins e.g. CAB61606.1|AL133210 putative export protein from Streptomyces coelicolor (496 aa); NP_108371.1|NC_002678 efflux pump protein FarB from Mesorhizobium loti (511 aa); P44927|EMRB_HAEINHI0897| multidrug resistance protein b homologue from Haemophilus influenzae (510 aa), FASTA scores: opt: 706, E(): 1.3e-36, (30.4% identity in 408 aa overlap); etc. Also similar to Rv2333c|MTCY3G12.01 from Mycobacterium tuberculosis (537 aa), FASTA score: (28.2% identity in 408 aa overlap); and Rv1410c|MTCY21B4.27c from Mycobacterium tuberculosis (518 aa), FASTA score: (26.8% identity in 496 aa overlap). Belongs to the major facilitator family; also known as the drug resistance translocase family. P71836 No cell wall and cell processes ML2224 MMAR_4905 Mb0805c NC_000962.3 Mycobrowser_v2 CDS 4400870 4401634 . + 0 Rv3912 Rv3912 Unknown Hypothetical alanine rich protein Rv3912, (MTV008.03), len: 254 aa. Hypothetical unknown ala-rich protein. Cleaved by Rip|Rv2869c, in M. tuberculosis Erdman (See Sklar et al., 2010). O53591 No conserved hypotheticals ML2702 MMAR_5476 MSMEG_6932 Mb3943 NC_000962.3 Mycobrowser_v2 CDS 1616961 1617386 . - 0 Rv1439c Rv1439c Unknown Unknown protein Rv1439c, (MTCY493.15), len: 141 aa. Unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06820 No conserved hypotheticals Mb1474c NC_000962.3 Mycobrowser_v2 RBS 1617390 1617393 . - 0 Rv1439c Rv1439c Unknown Unknown protein Rv1439c, (MTCY493.15), len: 141 aa. Unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06820 No conserved hypotheticals Mb1474c NC_000962.3 Mycobrowser_v2 CDS 879340 881040 . + 0 Rv0785 Rv0785 Function unknown Conserved protein Rv0785, (MTCY369.29), len: 566 aa. Conserved protein, highly similar to other conserved hypothetical proteins e.g. NP_105777.1| NC_002678 hypothetical protein from Mesorhizobium loti (552 aa); SC5F8.14|CAB93742.1|AL357613 conserved hypothetical protein from Streptomyces coelicolor (557 aa); AE001863|AE001863_31 from Deinococcus radiodurans (554 aa), FASTA scores: opt: 2243, E(): 0, (61.1% identity in 550 aa overlap); YEF7_YEAST|P32614 hypothetical 50.8 kd protein (470 aa), FASTA scores: opt: 169, E(): 0.0014, (23.8% identity in 542 aa overlap); etc. Also similar to Rv1817|MTCY1A11.26c from Mycobacterium tuberculosis (487 aa), FASTA score: (26.7% identity in 587 aa overlap). And shows similarity with other dehydrogenases. P71838 No conserved hypotheticals ML2222 MMAR_4632 MSMEG_5835 Mb0807,Mb0808 NC_000962.3 Mycobrowser_v2 CDS 4118530 4118691 . - 0 Rv3678A Rv3678A Function unknown Conserved hypothetical protein Rv3678A, len: 53 aa. Conserved hypothetical protein, similar to SCH17.10|AL079353_10 conserved hypothetical protein from Streptomyces coelicolor (53 aa), FASTA scores: opt: 259, E(): 1.5e-13, (78.0% identity in 50 aa overlap). Q8VIV6 No conserved hypotheticals MMAR_5167 MSMEG_6192 Mb3703c NC_000962.3 Mycobrowser_v2 CDS 884797 885840 . - 0 Rv0791c Rv0791c Function unknown Conserved protein Rv0791c, (MTV042.01c, MTCY369.35c), len: 347 aa. Conserved protein, similar (except in N-terminus) to others e.g. CAC44585.1|AL596162 conserved hypothetical protein from Streptomyces coelicolor (307 aa); NP_252643.1|NC_002516 hypothetical protein from Pseudomonas aeruginosa (364 aa); etc. Also some similarity with oxidoreductases e.g. AAK38097.1|AF323606_3|AF323606 putative F420-dependent dehydrogenase from Rhodococcus erythropolis (295 aa); etc. And also similar in part to other proteins from Mycobacterium tuberculosis e.g. Rv1855c|MTCY359.18|Z83859 (307 aa), FASTA scores: opt: 366, E(): 4e-16, (35.0% identity in 226 aa overlap); Rv3079c|MTCY22D7.02|Z83866 conserved hypothetical protein (275 aa), FASTA scores: opt: 342, E(): 1.2e-14, (31.6% identity in 234 aa overlap); Rv0044c possible oxidoreductase (264 aa). P71844 No conserved hypotheticals MMAR_2559 MSMEG_3301 Mb0815c NC_000962.3 Mycobrowser_v2 CDS 885837 886646 . - 0 Rv0792c Rv0792c Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably GntR-family) Rv0792c, (MTV042.02c), len: 269 aa. Probable transcriptional regulator, GntR-family, similar to many others of GntR family e.g. BSUB0018_189|Z99121 from Bacillus subtilis (243 aa), FASTA scores: opt: 367, E(): 1.5e-17, (32.1% identity in 246 aa overlap); P31453|YIDP_ECOLI from Escherichia coli (238 aa), FASTA scores: opt: 236, E(): 8.8e-09, (26.4% identity in 235 aa overlap); etc. O86331 No regulatory proteins MMAR_2560 MSMEG_4659 Mb0816c NC_000962.3 Mycobrowser_v2 RBS 886706 886712 . + 0 Rv0793 Rv0793 Function unknown Possible monooxygenase Rv0793, (MTV042.03), len: 101 aa. Possible monooxygenase (See Lemieux et al., 2005). Similar to e.g. NP_250888.1|NC_002516 hypothetical protein from Pseudomonas aeruginosa (114 aa); AE 001908|AE001908_7 hypothetical protein from Deinococcus radiodurans (101 aa), FASTA scores: opt: 215, E(): 3.1e-09, (40.4% identity in 99 aa overlap); NP_440966.1|NC_000911|D90908|PCC6803|D90908_2 unknown protein from Synechocystis sp. strain PCC 6803 (147 aa), FASTA scores: opt: 194, E(): 4.5e-08, (31.1% identity in 90 aa overlap); etc. Also similar to Rv2749|MTV002.14|AL0089|MTV002_15 conserved hypothetical protein from Mycobacterium tuberculosis (104 aa), FASTA scores: opt: 143, E(): 0.00026, (26.9% identity in 93 aa overlap). O86332 No intermediary metabolism and respiration MMAR_2561 Mb0817 NC_000962.3 Mycobrowser_v2 CDS 886719 887024 . + 0 Rv0793 Rv0793 Function unknown Possible monooxygenase Rv0793, (MTV042.03), len: 101 aa. Possible monooxygenase (See Lemieux et al., 2005). Similar to e.g. NP_250888.1|NC_002516 hypothetical protein from Pseudomonas aeruginosa (114 aa); AE 001908|AE001908_7 hypothetical protein from Deinococcus radiodurans (101 aa), FASTA scores: opt: 215, E(): 3.1e-09, (40.4% identity in 99 aa overlap); NP_440966.1|NC_000911|D90908|PCC6803|D90908_2 unknown protein from Synechocystis sp. strain PCC 6803 (147 aa), FASTA scores: opt: 194, E(): 4.5e-08, (31.1% identity in 90 aa overlap); etc. Also similar to Rv2749|MTV002.14|AL0089|MTV002_15 conserved hypothetical protein from Mycobacterium tuberculosis (104 aa), FASTA scores: opt: 143, E(): 0.00026, (26.9% identity in 93 aa overlap). O86332 No intermediary metabolism and respiration MMAR_2561 Mb0817 NC_000962.3 Mycobrowser_v2 CDS 922894 923709 . - 0 Rv0831c Rv0831c Function unknown Conserved protein Rv0831c, (MTV043.23c), len: 271 aa. Conserved protein, similar to Rv0347|MTY13E10_7|Z95324 conserved hypothetical protein from Mycobacterium tuberculosis (328 aa), FASTA scores: opt: 426, E(): 2.6e-21, (33.6% identity in 262 aa overlap). O53842 No conserved hypotheticals ML2180 Mb0854c NC_000962.3 Mycobrowser_v2 tRNA 923803 923875 . - 0 MTB000010 lysT - tRNA-Lys (TTT) lysT, tRNA-Lys, anticodon ttt, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v2 tRNA 923999 924072 . + 0 MTB000011 gluT - tRNA-Glu (TTC) gluT, tRNA-Glu, anticodon ttc, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 887137 888636 . - 0 Rv0794c Rv0794c Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv0794c, (MTV042.04c), len: 499 aa. Probable oxidoreductase, possibly dihydrolipoamide dehydrogenase or mercuric reductase. Highly similar to CAB62675.1|AL133422 probable oxidoreductase from Streptomyces coelicolor (477 aa); and similar to various oxidoreductases e.g. P08663|MERA_STAAU mercuric reductase (HG(II) reductase) from Staphylococcus aureus (547 aa); AAK70920.1|AC087551_19|AC087551 putative lipoamide dehydrogenase from Oryza sativa (563 aa); NP_437349.1|NC_003078 putative FAD-dependent pyridine nucleotide-disulphide oxidoreductase, similar to mercuric reductases protein from Sinorhizobium meliloti (473 aa); Q04829|DLDH_HALVO dihydrolipoamide dehydrogenase from Haloferax volcanii (475 aa); P08332|MERA_SHIFL mercuric reductase (564 aa), FASTA scores: opt: 522, E(): 3.7e-26, (31.7% identity in 467 aa overlap); P72740|DLDH_SYNY3|Q53395|LPDA|PDHD|SLR1096 dihydrolipoamide dehydrogenase from Synechocystis sp. strain PCC 6803 (474 aa), FASTA scores: opt: 602, E(): 2.3e-31, (31.0% identity in 493 aa overlap); etc. Note that previously known as lpdB. Q79FW1 No intermediary metabolism and respiration ML2216 MMAR_4894 MSMEG_4023 Mb0818c,Mb0819c NC_000962.3 Mycobrowser_v2 CDS 889072 889398 . + 0 Rv0795 Rv0795 Required for the transposition of the insertion element IS6110. Putative transposase for insertion sequence element IS6110 (fragment) Rv0795, (MTV042.05), len: 108 aa. Putative transposase for IS6110 (fragment), identical to Q50686 insertion element IS6110 (108 aa), FASTA score: (100.0 % identity in 108 aa overlap). The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv0795 and Rv0796, the sequence UUUUAAAG (directly upstream of Rv0796) maybe responsible for such a frameshifting event (see McAdam et al., 1990). P0C5G9 No insertion seqs and phages NC_000962.3 Mycobrowser_v2 CDS 889347 890333 . + 0 Rv0796 Rv0796 Required for the transposition of the insertion element IS6110. Putative transposase for insertion sequence element IS6110 Rv0796, (MTV042.06), len: 328 aa. Putative transposase for IS6110. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv0795 and Rv0796, the sequence UUUUAAAG (directly upstream of Rv0796) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 50 aa). P0C5G8 No insertion seqs and phages NC_000962.3 Mycobrowser_v2 CDS 1261922 1262158 . + 0 Rv1134 Rv1134 Unknown Hypothetical protein Rv1134, (MTCI65.01), len: 78 aa. Hypothetical unknown protein. O06534 No conserved hypotheticals Mb1165 NC_000962.3 Mycobrowser_v2 CDS 890388 891482 . + 0 Rv0797 Rv0797 Required for the transposition of the insertion element IS1547. Putative transposase for insertion sequence element IS1547 Rv0797, (MTCI249B.03c, MTV042.07), len: 364 aa. Putative transposase for IS1547; almost identical to (but 20 aa shorter than) Y13470|MTY13470_2 from Mycobacterium tuberculosis (383 aa). Also similar to other transposases e.g. MAIS1110A _1|Q48909 transposase from Mycobacterium avium (464 aa), FASTA scores: opt: 226, E(): 2.4e-08, (30.7% identity in 199 aa overlap). Also slight similarity to Rv2014|MTCY39.03c from Mycobacterium tuberculosis (222 aa), FASTA score: (24.8% identity in 141 aa overlap). O07182 No insertion seqs and phages Mb0820 NC_000962.3 Mycobrowser_v2 CDS 891472 892269 . - 0 Rv0798c cfp29 Unknown. Supposedly released from the envelope to the outside during growth. 29 KDa antigen CFP29 Rv0798c, (MTCI429B.02), len: 265 aa. Cfp29, 29 kDa antigen (see citations below). Highly similar to Q45296|BLLINM18P_1|CAA63787.1|X93588 linocin M18 from Brevibacterium linens (266 aa), FASTA scores: (58.5% identity in 265 aa overlap). Also shows similarity with NP_228594.1|NC_000853 bacteriocin from Thermotoga maritima (262 aa). O07181 No virulence, detoxification, adaptation MMAR_4893 MSMEG_5830 Mb0821c NC_000962.3 Mycobrowser_v2 RBS 892278 892282 . - 0 Rv0798c cfp29 Unknown. Supposedly released from the envelope to the outside during growth. 29 KDa antigen CFP29 Rv0798c, (MTCI429B.02), len: 265 aa. Cfp29, 29 kDa antigen (see citations below). Highly similar to Q45296|BLLINM18P_1|CAA63787.1|X93588 linocin M18 from Brevibacterium linens (266 aa), FASTA scores: (58.5% identity in 265 aa overlap). Also shows similarity with NP_228594.1|NC_000853 bacteriocin from Thermotoga maritima (262 aa). O07181 No virulence, detoxification, adaptation MMAR_4893 MSMEG_5830 Mb0821c NC_000962.3 Mycobrowser_v2 tRNA 924110 924183 . + 0 MTB000012 aspT - tRNA-Asp (GTC) aspT, tRNA-Asp, anticodon gtc, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 892266 893273 . - 0 Rv0799c Rv0799c Function unknown Conserved protein Rv0799c, (MTCY07H7A.10, MTCI429B.01), len: 335 aa. Conserved protein, similar to Q50021|U2266C from Mycobacterium leprae (146 aa), FASTA scores: opt: 147, E(): 0.0016, (33.3% identity in 117 aa overlap); Q50020|U2266B from Mycobacterium leprae (27 aa), FASTA scores: opt: 94, E(): 1.3, (56.5% identity in 23 aa overlap). Also highly similar to others e.g. CAC01593.1|AL391041 conserved hypothetical protein from Streptomyces coelicolor (316 aa); AF088897|AF088897_9 hypothetical protein from Zymomonas mobilis (322 aa), FASTA scores: opt: 1132, E(): 0, (56.1% identity in 303 aa overlap); P76536|ECAE000330_8 hypothetical protein from Escherichia coli strain K-12 (308 aa), FASTA scores: E(): 2.2e-30, (37.4% identity in 297 aa overlap); etc. Also similar to some tyrA proteins. Predicted to be an outer membrane protein (See Song et al., 2008). O07180 No conserved hypotheticals ML2214 MMAR_4892 MSMEG_5829 Mb0822c NC_000962.3 Mycobrowser_v2 CDS 893318 894619 . + 0 Rv0800 pepC Function unknown; possibly hydrolyzes peptides and/or proteins. Probable aminopeptidase PepC Rv0800, (MTCY07H7A.09c), len: 433 aa. Probable pepC, aminopeptidase I, highly similar (but shorter 17 aa) to Q50022|PEPX aminopeptidase from Mycobacterium leprae (443 aa), FASTA scores: opt: 2237, E(): 0, (78.3% identity in 433 aa overlap). Also highly similar to others from Eukaryotes and bacteria, e.g. T36482 probable aminopeptidase from Streptomyces coelicolor (432 aa), P14904|AMPL_YEAST vacuolar aminopeptidase I precursor from Saccharomyces cerevisiae (514 aa), FASTA scores: opt: 425, E(): 4.8e-21, (31.0% identity in 445 aa overlap); etc. Also similar to hypothetical proteins e.g. P38821|YHR3_YEAST hypothetical 54.2 kDa protein from Saccharomyces cerevisiae (490 aa), FASTA scores: opt: 429, E(): 2.5e-21, (34.8% identity in 443 aa overlap); etc. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). O06634 No intermediary metabolism and respiration ML2213 MMAR_4891 MSMEG_5828 Mb0823 NC_000962.3 Mycobrowser_v2 CDS 894631 894978 . + 0 Rv0801 Rv0801 Function unknown Conserved protein Rv0801, (MTCY07H7A.08c), len: 115 aa. Conserved protein, similar to many hypothetical proteins from Streptomyces sp. e.g. SCD840A.20|AB81865.1|AL161691 hypothetical protein from Streptomyces coelicolor (145 aa); AF072709|AF072709_8 from Streptomyces lividans (131 aa), FASTA scores: opt: 120, E(): 0.2, (26.3% identity in 118 aa overlap); etc. O06633 No conserved hypotheticals MMAR_4890 MSMEG_5827 Mb0824 NC_000962.3 Mycobrowser_v2 CDS 4387365 4387895 . - 0 Rv3902c Rv3902c Unknown Hypothetical protein Rv3902c, (MTCY15F10.10), len: 176 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O05443 No conserved hypotheticals Mb3932c NC_000962.3 Mycobrowser_v2 CDS 894972 895628 . - 0 Rv0802c Rv0802c Succinylation, substrate unknown Possible succinyltransferase in the GCN5-related N-acetyltransferase family Rv0802c, (MTCY07H7A.07c), len: 218 aa. Possible succinyltransferase in the GNAT (Gcn5-related N-acetyltransferase) family (See Vetting et al., 2008). Shows partial similarity with many acetyltransferases and hypothetical proteins e.g. P96579|BSUB0003_68 probable acetyltransferase from Bacillus subtilis (183 aa), FASTA scores: E(): 0.0044, (26.4% identity in 110 aa overlap). O06632 No intermediary metabolism and respiration ML2212 MMAR_4889 Mb0825c NC_000962.3 Mycobrowser_v2 CDS 916477 917646 . - 0 Rv0823c Rv0823c Thought to be involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0823c, (MTV043.15c), len: 389 aa. Possible transcriptional regulator (resembles nitrogen regulation protein), equivalent (but longer 24 aa in N-terminus) to MLU15182_31|U15182|NtrB NtrB protein from Mycobacterium leprae (384 aa), FASTA scores: opt: 2070, E(): 0, (82.3% identity in 384 aa overlap) (see citation below). Also highly similar to CAB63312.1|AL133471|SCC82.03c hypothetical protein from Streptomyces coelicolor (406 aa); and to many transcriptional regulators members of UPF0034 family (NIFR3/SMM1) e.g. D26185|BAC180K_143 protein similar to transcriptional regulator (nitrogen regulation protein) from Bacillus subtilis (333 aa), FASTA scores: opt: 609, E(): 1.4e-32, (38.3% identity in 326 aa overlap); NP_349795.1|NC_003030 NifR3 family enzyme from Clostridium acetobutylicum (321 aa); etc. Contains PS01136 Uncharacterized protein family UPF0034 signature. O53835 No regulatory proteins ML2186 MMAR_4857 MSMEG_5774 Mb0846c NC_000962.3 Mycobrowser_v2 CDS 908181 908483 . - 0 Rv0814c sseC2 Thought to be involved in sulphur metabolism. Conserved protein SseC2 Rv0814c, (MTV043.06c, O05794), len: 100 aa. SseC2, conserved protein, highly similar to AAA62972.1|U15182|MLU15182_17 hypothetical protein from Mycobacterium leprae (143 aa), FASTA scores: opt: 545, E(): 0, (84.0% identity in 100 aa overlap); and NP_302441.1|NC_002677|Z95150|MTCY164_29 conserved hypothetical protein from Mycobacterium leprae (100 aa), FASTA scores: opt: 647, E(): 0, (100.0% identity in 100 aa overlap). Also highly similar to M29612|SERCYSA_5 rhodanese-like protein from Saccharopolyspora erythraea (101 aa), FASTA scores: opt: 345, E(): 1.2e-18, (57.1% identity in 98 aa overlap); and similar at the C-terminus to the C-terminus of CAB94069.1|AL358692 conserved hypothetical protein from Streptomyces coelicolor (95 aa). Identical second copy present as Rv3118|MTCY164.28|SSEC1 from Mycobacterium tuberculosis (100 aa) (100.0% identity in 100 aa overlap). P0CG95 No intermediary metabolism and respiration ML2199 Mb0837c NC_000962.3 Mycobrowser_v2 RBS 908490 908494 . - 0 Rv0814c sseC2 Thought to be involved in sulphur metabolism. Conserved protein SseC2 Rv0814c, (MTV043.06c, O05794), len: 100 aa. SseC2, conserved protein, highly similar to AAA62972.1|U15182|MLU15182_17 hypothetical protein from Mycobacterium leprae (143 aa), FASTA scores: opt: 545, E(): 0, (84.0% identity in 100 aa overlap); and NP_302441.1|NC_002677|Z95150|MTCY164_29 conserved hypothetical protein from Mycobacterium leprae (100 aa), FASTA scores: opt: 647, E(): 0, (100.0% identity in 100 aa overlap). Also highly similar to M29612|SERCYSA_5 rhodanese-like protein from Saccharopolyspora erythraea (101 aa), FASTA scores: opt: 345, E(): 1.2e-18, (57.1% identity in 98 aa overlap); and similar at the C-terminus to the C-terminus of CAB94069.1|AL358692 conserved hypothetical protein from Streptomyces coelicolor (95 aa). Identical second copy present as Rv3118|MTCY164.28|SSEC1 from Mycobacterium tuberculosis (100 aa) (100.0% identity in 100 aa overlap). P0CG95 No intermediary metabolism and respiration ML2199 Mb0837c NC_000962.3 Mycobrowser_v2 CDS 895820 898084 . + 0 Rv0803 purL Involved in de novo purine biosynthesis (at the fourth step) [catalytic activity: ATP + 5'-phosphoribosylformylglycinamide + L-glutamine + H(2)O = ADP + phosphate + 5'-phosphoribosylformylglycinamidine + L-glutamate]. Phosphoribosylformylglycinamidine synthase II PurL (FGAM synthase II) Rv0803, (MTCY07H7A.06c), len: 754 aa. PurL, phosphoribosylformylglycinamidine synthase II (see citations below), equivalent to NP_302451.1|NC_002677 phosphoribosylformylglycinamidine synthase II from Mycobacterium leprae (754 aa). Also highly similar to others e.g. Q9RKK5|PURL_STRCO from Streptomyces coelicolor (752 aa); P12042|PURL_BACSU from Bacillus subtilis (742 aa), FASTA score: (44.7% identity in 716 aa); etc. Start was chosen by similarity. Belongs to the FGAMS family. P0A5T8 No intermediary metabolism and respiration ML2211 MMAR_4888 MSMEG_5824 Mb0826 NC_000962.3 Mycobrowser_v2 CDS 898081 898710 . + 0 Rv0804 Rv0804 Function unknown Conserved hypothetical protein Rv0804, (MTCY07H7A.05c), len: 209 aa. Conserved hypothetical protein, showing similarity with C-terminus of Rv1863c|MTCY359.10 conserved hypothetical protein from Mycobacterium tuberculosis (256 aa), FASTA scores: opt: 199, E(): 1.2e-05, (33.2% identity in 220 aa overlap); and Rv0658c. Contains PS01151 Fimbrial biogenesis outer membrane usher protein signature. O06630 No conserved hypotheticals MMAR_4887 MSMEG_5821 Mb0827 NC_000962.3 Mycobrowser_v2 CDS 898831 899787 . + 0 Rv0805 Rv0805 Hydrolyzes cyclic nucleotide monophosphate to nucleotide monophosphate. Shown to hydrolyze 2',3'-cNMP and 3',5'-cNMP. Class III cyclic nucleotide phosphodiesterase (cNMP PDE) Rv0805, (MTCY07H7A.04c), len: 318 aa. Cyclic nucleotide phosphodiesterase (cNMP PDE) (See Shenoy et al., 2005), member of binuclear metallophosphoesterase superfamily, equivalent to Q50024 from Mycobacterium leprae (317 aa), FASTA scores: opt: 1713, E(): 0, (82.5% identity in 315 aa overlap). Also shows similarity with hypothetical proteins and icc proteins e.g. SC9B1.22c|T35867 hypothetical protein from Streptomyces coelicolor (305 aa); P36650|ICC_ECOLI icc protein from Escherichia coli (275 aa), FASTA scores: opt: 310, E(): 8.9e-14, (31.3% identity in 214 aa overlap); etc. O06629 No intermediary metabolism and respiration ML2210 MMAR_4886 Mb0828 NC_000962.3 Mycobrowser_v2 CDS 909611 910033 . - 0 Rv0816c thiX Thioredoxin participates in various redox reactions through the reversible oxidation of its active center dithiol, to a disulfide, & catalyzes dithiol-disulfide exchange reactions. Probable thioredoxin ThiX Rv0816c, (MTV043.08c), len: 140 aa. Probable thiX, thioredoxin, equivalent to ThiX|U15182|MLU15182_21 thioredoxin from Mycobacterium leprae (172 aa), FASTA scores: opt: 556, E(): 8.8e-31, (63.8% identity in 141 aa overlap); and similar to AAL08576.1|AF418548_2|AF418548 thioredoxin from Mycobacterium avium subsp. paratuberculosis (117 aa). Also similar to other bacterial thioredoxins e.g. CAB95303.1|AL359779 putative thioredoxin from Streptomyces coelicolor (126 aa); P33791|THIO_STRAU|TRX|TRXA thioredoxin from Streptomyces aureofaciens (106 aa); etc. And similar to Rv3914|MT4033|MTV028.05|NP_218431.1|NC_000962|trxC thioredoxin (TRX) (MPT46) from Mycobacterium tuberculosis (116 aa). Has hydrophobic stretch at N-terminus. Seems to belong to the thioredoxin family. O53828 No intermediary metabolism and respiration ML2196 MMAR_3647 MSMEG_5786 Mb0839c NC_000962.3 Mycobrowser_v2 CDS 899732 901330 . - 0 Rv0806c cpsY Thought to be involved in exopolysaccharide and/or lipopolysaccharide biosynthetic pathway [catalytic activity: UDP-glucose = UDP-galactose]. Possible UDP-glucose-4-epimerase CpsY (galactowaldenase) (UDP-galactose-4-epimerase) (uridine diphosphate galactose-4-epimerase) (uridine diphospho-galactose-4-epimerase) Rv0806c, (MTCY07H7A.03), len: 532 aa. Possible cpsY, UDP-glucose-4-epimerase, equivalent to Q50025|CPSY probable UDP-glucose-4-epimerase from Mycobacterium leprae (542 aa), FASTA scores: opt: 2964, E(): 0, (82.3% identity in 530 aa overlap). Also similar to AAC38286.1|AF019760|SACB CpsY homolog (involved in meningococcal capsule biosynthesis) from Neisseria meningitidis serogroup a (545 aa); Q51151 capsule gene complex UPD-glucose-4-epimerase (gale) from Neisseria meningitidis (373 aa), FASTA scores: opt: 496, E(): 9.5e-27, (29.3% identity in 358 aa overlap); C-terminus of CAB75373.1|AL139298 putative transferase from Streptomyces coelicolor (942 aa); and many hypothetical proteins from Streptomyces coelicolor. Seems to belong to the sugar epimerase family. O06628 No cell wall and cell processes ML2209 MMAR_4885 Mb0829c NC_000962.3 Mycobrowser_v2 CDS 901635 902024 . + 0 Rv0807 Rv0807 Function unknown Conserved hypothetical protein Rv0807, (MTCY07H7A.02c), len: 129 aa. Conserved hypothetical protein, equivalent to O05761|MLCB5_31 hypothetical 14.0 kDa protein from Mycobacterium leprae (131 aa), FASTA scores: E(): 0, (73.4% identity in 128 aa overlap). Also highly similar to BAA89438.1|AB003158|ORF3 hypothetical protein from Corynebacterium ammoniagenes (132 aa); and C-terminus of SCD25.20|CAB56364.1|AL118514 hypothetical protein from Streptomyces coelicolor (202 aa). O06627 No conserved hypotheticals ML2207 MMAR_4882 MSMEG_5817 Mb0830 NC_000962.3 Mycobrowser_v2 CDS 1279998 1280846 . - 0 Rv1153c omt Function unknown, but supposedly involved in lipid metabolism Probable O-methyltransferase Omt Rv1153c, (MTCI65.20c), len: 282 aa. Probable omt, O-methyltransferase, similar to TCMP_STRGA|P39887 Tetracenomycin polyketide synthesis O-methyltransferase tcmP from Streptomyces glaucescens (270 aa), FASTA scores: opt: 368, E(): 1.7e-17, (31.3% identity in 233 aa overlap). O06551 No lipid metabolism MMAR_4300 MSMEG_5173 Mb1184c NC_000962.3 Mycobrowser_v2 RBS 1280854 1280857 . - 0 Rv1153c omt Function unknown, but supposedly involved in lipid metabolism Probable O-methyltransferase Omt Rv1153c, (MTCI65.20c), len: 282 aa. Probable omt, O-methyltransferase, similar to TCMP_STRGA|P39887 Tetracenomycin polyketide synthesis O-methyltransferase tcmP from Streptomyces glaucescens (270 aa), FASTA scores: opt: 368, E(): 1.7e-17, (31.3% identity in 233 aa overlap). O06551 No lipid metabolism MMAR_4300 MSMEG_5173 Mb1184c NC_000962.3 Mycobrowser_v2 CDS 917734 918750 . - 0 Rv0824c desA1 Catalyzes the principal conversion of saturated fatty acids to unsaturated fatty acids. Thought to convert stearoyl-ACP to oleoyl-ACP by introduction of a cis double bond between carbons delta-9 and delta-10 of the acyl chain [catalytic activity: stearoyl-[acyl-carrier protein] + AH2 + O2 = oleoyl-[acyl-carrier protein] + a + 2 H2O]. Probable acyl-[acyl-carrier protein] desaturase DesA1 (acyl-[ACP] desaturase) (stearoyl-ACP desaturase) (protein Des) Rv0824c, (MTV043.16c), len: 338 aa. Probable desA1 (alternate gene name: des), acyl-[acyl-carrier protein] desaturase (stearoyl-ACP desaturase) (see Jackson et al., 1997), equivalent to U15182|MLU15182_32 acyl-[ACP] desaturase from Mycobacterium leprae (338 aa), FASTA scores: opt: 1880, E(): 0, (79.9% identity in 338 aa overlap); and highly similar in part to fragment CAB96061.1|AJ250019 Steroyl-ACP-desaturase from Mycobacterium avium subsp. paratuberculosis (93 aa). Also similar to other fatty acid desaturases e.g. T35035 probable acyl-[acyl-carrier protein] desaturase from Streptomyces coelicolor (328 aa); Q40731|STAD_ORYSA acyl-[acyl-carrier protein] desaturase precursor from Oryza sativa (Rice) (390 aa); etc. Also highly similar to desA2|Rv1094 from Mycobacterium tuberculosis (275 aa). Contains PS00225 Crystallins beta and gamma 'Greek key' motif signature. Belongs to the fatty acid desaturase family. Cofactor: ferredoxin, ferredoxin NADPH reductase, and NADPH. Predicted possible vaccine candidate (See Zvi et al., 2008). Q50824 No lipid metabolism ML2185 MMAR_4856 MSMEG_5773 Mb0847c NC_000962.3 Mycobrowser_v2 RBS 918755 918759 . - 0 Rv0824c desA1 Catalyzes the principal conversion of saturated fatty acids to unsaturated fatty acids. Thought to convert stearoyl-ACP to oleoyl-ACP by introduction of a cis double bond between carbons delta-9 and delta-10 of the acyl chain [catalytic activity: stearoyl-[acyl-carrier protein] + AH2 + O2 = oleoyl-[acyl-carrier protein] + a + 2 H2O]. Probable acyl-[acyl-carrier protein] desaturase DesA1 (acyl-[ACP] desaturase) (stearoyl-ACP desaturase) (protein Des) Rv0824c, (MTV043.16c), len: 338 aa. Probable desA1 (alternate gene name: des), acyl-[acyl-carrier protein] desaturase (stearoyl-ACP desaturase) (see Jackson et al., 1997), equivalent to U15182|MLU15182_32 acyl-[ACP] desaturase from Mycobacterium leprae (338 aa), FASTA scores: opt: 1880, E(): 0, (79.9% identity in 338 aa overlap); and highly similar in part to fragment CAB96061.1|AJ250019 Steroyl-ACP-desaturase from Mycobacterium avium subsp. paratuberculosis (93 aa). Also similar to other fatty acid desaturases e.g. T35035 probable acyl-[acyl-carrier protein] desaturase from Streptomyces coelicolor (328 aa); Q40731|STAD_ORYSA acyl-[acyl-carrier protein] desaturase precursor from Oryza sativa (Rice) (390 aa); etc. Also highly similar to desA2|Rv1094 from Mycobacterium tuberculosis (275 aa). Contains PS00225 Crystallins beta and gamma 'Greek key' motif signature. Belongs to the fatty acid desaturase family. Cofactor: ferredoxin, ferredoxin NADPH reductase, and NADPH. Predicted possible vaccine candidate (See Zvi et al., 2008). Q50824 No lipid metabolism ML2185 MMAR_4856 MSMEG_5773 Mb0847c NC_000962.3 Mycobrowser_v2 CDS 1143736 1144467 . + 0 Rv1022 lpqU Unknown Probable conserved lipoprotein LpqU Rv1022, (MTCY10G2.27c), len: 243 aa. Probable lpqU conserved lipoprotein. Similar to Mycobacterium tuberculosis hypothetical protein Rv1230c|MTV006.02C, FASTA scores: E(): 2.8e-18, (37.9% identity in 240 aa overlap). Similar to AL133423|SC4A7.37 hypothetical protein from Streptomyces coelicolor (421 aa), FASTA scores: opt: 474, E(): 2.7e-21, (42.2% identity in 211 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. P96378 No cell wall and cell processes ML0254 MMAR_4463 MSMEG_5416 Mb1050 NC_000962.3 Mycobrowser_v2 tRNA 924213 924286 . + 0 MTB000013 pheU - tRNA-Phe (GAA) pheU, tRNA-Phe, anticodon gaa, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 1402778 1403386 . - 0 Rv1255c Rv1255c Involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv1255c, (MTCY50.27), len: 202 aa. Possible regulatory protein, similar to others e.g. ACRR_ECOLI|P34000 potential acrab operon repressor from E. coli (215 aa), FASTA scores: opt: 128, E(): 0.25, (42.1% identity in 57 aa overlap). Helix turn helix motif present at aa 36-57 (+5.48 SD). Q11063 No regulatory proteins MMAR_4185 MSMEG_5040 Mb1287c NC_000962.3 Mycobrowser_v2 CDS 902111 903694 . + 0 Rv0808 purF Involved in de novo purine biosynthesis (at the first step) [catalytic activity: 5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O]. Amidophosphoribosyltransferase PurF (glutamine phosphoribosylpyrophosphate amidotransferase) (ATASE) (gpatase) Rv0808, (MTCY07H7A.01c), len: 527 aa. PurF, amidophosphoribosyltransferase, equivalent to MLCB5_32|Q50028|PURF from Mycobacterium leprae (556 aa), FASTA scores: (91.3% identity in 518 aa overlap); and CAB96578.1|AJ278609 phosphoribosyl pyrophosphate amidotransferase from Mycobacterium smegmatis (511 aa)(see citation below). Also highly similar to others e.g. BAA89439.1|AB003158 amidophosphoribosyl transferase from Corynebacterium ammoniagenes (490 aa); P00497|PUR1_BACSU amidophosphoribosyltransferase precursor from Bacillus subtilis (476 aa), FASTA scores: opt: 1412, E(): 0, (46.2% identity in 470 aa overlap); etc. Contains PS00103 Purine/pyrimidine phosphoribosyl transferases signature. Belongs to the purine/pyrimidine phosphoribosyltransferase family. P65829 No intermediary metabolism and respiration ML2206 MMAR_4881 MSMEG_5800 Mb0831 NC_000962.3 Mycobrowser_v2 CDS 903725 904819 . + 0 Rv0809 purM Involved in de novo purine biosynthesis (at the fifth step) [catalytic activity: ATP + 5'-phosphoribosylformylglycinamidine = ADP + phosphate + 5'-phosphoribosyl-5-aminoimidazole]. Probable phosphoribosylformylglycinamidine CYCLO-ligase PurM (AIRS) (phosphoribosyl-aminoimidazole synthetase) (air synthase) Rv0809, (MTV043.01), len: 364 aa. Probable purM, 5'-phosphoribosyl-5-aminoimidazole synthetase, equivalent to NP_302446.1|NC_002677 5'-phosphoribosyl-5-aminoimidazole synthase from Mycobacterium leprae (364 aa). Also highly similar to many e.g. P12043|PUR5_BACSU phosphoribosylformylglycinamidine CYCLO-ligase from Bacillus subtilis (346 aa), FASTA scores: opt: 1023, E(): 0, (46.5% identity in 331 aa overlap); U68765|STU68765_2 from Salmonella typhimurium (345 aa), FASTA scores: opt: 1014, E():0, (47.6% identity in 330 aa overlap); etc. O53823 No intermediary metabolism and respiration ML2205 MMAR_4880 MSMEG_5798 Mb0832 NC_000962.3 Mycobrowser_v2 CDS 904905 905087 . - 0 Rv0810c Rv0810c Function unknown Conserved hypothetical protein Rv0810c, (MTV043.02c), len: 60 aa. Conserved hypothetical protein, with its N-terminus highly similar to NP_302445.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (62 aa); and AL118514|SCD25_24 hypothetical protein from Streptomyces coelicolor (84 aa), FASTA scores: opt: 180, E(): 5.7e-07, (51.8% identity in 56 aa overlap). O53824 No conserved hypotheticals ML2204 MMAR_4879 MSMEG_5797 Mb0833c NC_000962.3 Mycobrowser_v2 RBS 905095 905100 . - 0 Rv0810c Rv0810c Function unknown Conserved hypothetical protein Rv0810c, (MTV043.02c), len: 60 aa. Conserved hypothetical protein, with its N-terminus highly similar to NP_302445.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (62 aa); and AL118514|SCD25_24 hypothetical protein from Streptomyces coelicolor (84 aa), FASTA scores: opt: 180, E(): 5.7e-07, (51.8% identity in 56 aa overlap). O53824 No conserved hypotheticals ML2204 MMAR_4879 MSMEG_5797 Mb0833c NC_000962.3 Mycobrowser_v2 CDS 905234 906340 . - 0 Rv0811c Rv0811c Function unknown Conserved protein Rv0811c, (MTV043.03c), len: 368 aa. Conserved protein, equivalent to U2266F|U15182|MLU15182_13 hypothetical protein from Mycobacterium leprae (366 aa), FASTA scores: opt: 1870, E(): 0, (77.4% identity in 367 aa overlap). Also highly similar to BAA89441.1|AB003158|ORF4 hypothetical protein from Corynebacterium ammoniagenes (359 aa); and CAB94085.1|AL358692 conserved hypothetical protein from Streptomyces coelicolor (321 aa). O53825 No conserved hypotheticals ML2203 MMAR_4878 MSMEG_5796 Mb0834c NC_000962.3 Mycobrowser_v2 ncRNA 918264 918458 . + 0 MTB000053 ASdes Unknown Putative small regulatory RNA ASdes, putative small regulatory RNA (See Arnvig and Young, 2009). Alternate 5'-ends at positions 918350 and 918365. Alternate 3'-ends at positions 918432 and 918412. No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 910030 910842 . - 0 Rv0817c Rv0817c Unknown Probable conserved exported protein Rv0817c, (MTV043.09c), len: 270 aa. Probable conserved exported protein, with N-terminal signal sequence, equivalent (but shorter 13 aa) to U15182|MLU15182_22|U2266M probable exported protein from Mycobacterium leprae (283 aa), FASTA scores: opt: 1287, E(): 0, (73.0% identity in 270 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). O53829 No cell wall and cell processes ML2195 MMAR_4866 MSMEG_5785 Mb0840c NC_000962.3 Mycobrowser_v2 RBS 906410 906413 . + 0 Rv0812 Rv0812 Acts on amino acids. Probable amino acid aminotransferase Rv0812, (MTV043.04), len: 289 aa. Probable amino acid aminotransferase, similar to other amino acid aminotransferases, generelly class-IV of pyridoxal-phosphate-dependent aminotransferases, and especially ILVE proteins and PABC proteins e.g. B76065.1|AL157953 putative aminotransferase from Streptomyces coelicolor (273 aa); NP_069766.1|NC_000917 branched-chain amino acid aminotransferase (ilvE) from Archaeoglobus fulgidus (290 aa); P54692|DAAA_BACLI D-alanine aminotransferase from Bacillus licheniformis (283 aa); P28305|PABC_ECOLI|B1096 4-amino-4-deoxychorismate lyase (ADC lyase) From Escherichia coli strain K12 (269 aa), FASTA scores: opt: 165, E(): 0.00064, (26.8% identity in 198 aa overlap); etc. Note that previously known as pabC. Q79FW0 No intermediary metabolism and respiration ML2202 MMAR_4873 MSMEG_5795 Mb0835 NC_000962.3 Mycobrowser_v2 CDS 906423 907292 . + 0 Rv0812 Rv0812 Acts on amino acids. Probable amino acid aminotransferase Rv0812, (MTV043.04), len: 289 aa. Probable amino acid aminotransferase, similar to other amino acid aminotransferases, generelly class-IV of pyridoxal-phosphate-dependent aminotransferases, and especially ILVE proteins and PABC proteins e.g. B76065.1|AL157953 putative aminotransferase from Streptomyces coelicolor (273 aa); NP_069766.1|NC_000917 branched-chain amino acid aminotransferase (ilvE) from Archaeoglobus fulgidus (290 aa); P54692|DAAA_BACLI D-alanine aminotransferase from Bacillus licheniformis (283 aa); P28305|PABC_ECOLI|B1096 4-amino-4-deoxychorismate lyase (ADC lyase) From Escherichia coli strain K12 (269 aa), FASTA scores: opt: 165, E(): 0.00064, (26.8% identity in 198 aa overlap); etc. Note that previously known as pabC. Q79FW0 No intermediary metabolism and respiration ML2202 MMAR_4873 MSMEG_5795 Mb0835 NC_000962.3 Mycobrowser_v2 CDS 907338 908018 . - 0 Rv0813c Rv0813c Function unknown Conserved protein Rv0813c, (MTV043.05c), len: 226 aa. Conserved protein, highly similar to U15182|MLU15182_16 hypothetical protein from Mycobacterium leprae (242 aa), FASTA scores: opt: 1191, E(): 0, (78.3% identity in 226 aa overlap); and NP_302442.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (228 aa). Also similar to AB94083.1|AL358692|SCD66.16 hypothetical protein from Streptomyces coelicolor (191 aa); and Rv2717c|MTCY05A6_37 hypothetical protein from Mycobacterium tuberculosis (164 aa), FASTA score: (30.4% identity in 171 aa overlap). Possibly a new bacterial family of fatty acid-binding protein-like proteins (See Shepard et al., 2007). O53827 No conserved hypotheticals ML2200 MMAR_4871 MSMEG_5792 Mb0836c NC_000962.3 Mycobrowser_v2 CDS 918912 919553 . - 0 Rv0825c Rv0825c Function unknown Conserved protein Rv0825c, (MTV043.17c), len: 213 aa. Conserved protein, highly similar, but in part (between aa ~43-96) to fadD27|Rv0275c|MTV035.03 putative fatty-acid-CoA ligase from Mycobacterium tuberculosis (241 aa), FASTA scores: E(): 7.3e-09, (32.6% identity in 190 aa overlap). Also shows similarity with other proteins from Mycobacterium tuberculosis e.g. Rv0078|AL0214|MTV030_22 (201 aa), FASTA scores: opt:118, E(): 0.32, (34.5% identity in 113 aa overlap); etc. O53836 No conserved hypotheticals ML2184 MMAR_4855 MSMEG_5768 Mb0848c NC_000962.3 Mycobrowser_v2 RBS 919558 919562 . - 0 Rv0825c Rv0825c Function unknown Conserved protein Rv0825c, (MTV043.17c), len: 213 aa. Conserved protein, highly similar, but in part (between aa ~43-96) to fadD27|Rv0275c|MTV035.03 putative fatty-acid-CoA ligase from Mycobacterium tuberculosis (241 aa), FASTA scores: E(): 7.3e-09, (32.6% identity in 190 aa overlap). Also shows similarity with other proteins from Mycobacterium tuberculosis e.g. Rv0078|AL0214|MTV030_22 (201 aa), FASTA scores: opt:118, E(): 0.32, (34.5% identity in 113 aa overlap); etc. O53836 No conserved hypotheticals ML2184 MMAR_4855 MSMEG_5768 Mb0848c NC_000962.3 Mycobrowser_v2 CDS 908485 909318 . - 0 Rv0815c cysA2 May be a sulfotransferase involved in the formation of thiosulfate [catalytic activity: thiosulfate + cyanide = sulfite + thiocyanate]. Probable thiosulfate sulfurtransferase CysA2 (rhodanese-like protein) (thiosulfate cyanide transsulfurase) (thiosulfate thiotransferase) Rv0815c, (MTV043.07c, MT0837, O05793), len: 277 aa. Probable cysA2 (alternate gene name: sseC4), thiosulfate sulfurtransferase (see Wooff et al., 2002), equivalent to Q50036|CYSA|CYSA3|ML2198|THTR_MYCLE putative sulfurtransferase thiosulfate from Mycobacterium leprae (277 aa). Also highly similar to other putative thiosulfate sulfurtransferases e.g. P16385|THTR_SACER putative thiosulfate sulfurtransferase from Saccharopolyspora erythraea (Streptomyces erythraeus) (281 aa); NP_293941.1|NC_001263 thiosulfate sulfurtransferase from Deinococcus radiodurans (286 aa); etc. Identical second copy present as Rv3117|MTCY164.27|MT3199|O05793|cysA3 (277 aa) (100.0% identity in 277 aa overlap). Contains PS00683 Rhodanese C-terminal signature at C-terminus. Belongs to the rhodanese family. O05793 No intermediary metabolism and respiration ML2198 MMAR_4868 MSMEG_5789 Mb0838c NC_000962.3 Mycobrowser_v2 RBS 909327 909331 . - 0 Rv0815c cysA2 May be a sulfotransferase involved in the formation of thiosulfate [catalytic activity: thiosulfate + cyanide = sulfite + thiocyanate]. Probable thiosulfate sulfurtransferase CysA2 (rhodanese-like protein) (thiosulfate cyanide transsulfurase) (thiosulfate thiotransferase) Rv0815c, (MTV043.07c, MT0837, O05793), len: 277 aa. Probable cysA2 (alternate gene name: sseC4), thiosulfate sulfurtransferase (see Wooff et al., 2002), equivalent to Q50036|CYSA|CYSA3|ML2198|THTR_MYCLE putative sulfurtransferase thiosulfate from Mycobacterium leprae (277 aa). Also highly similar to other putative thiosulfate sulfurtransferases e.g. P16385|THTR_SACER putative thiosulfate sulfurtransferase from Saccharopolyspora erythraea (Streptomyces erythraeus) (281 aa); NP_293941.1|NC_001263 thiosulfate sulfurtransferase from Deinococcus radiodurans (286 aa); etc. Identical second copy present as Rv3117|MTCY164.27|MT3199|O05793|cysA3 (277 aa) (100.0% identity in 277 aa overlap). Contains PS00683 Rhodanese C-terminal signature at C-terminus. Belongs to the rhodanese family. O05793 No intermediary metabolism and respiration ML2198 MMAR_4868 MSMEG_5789 Mb0838c NC_000962.3 Mycobrowser_v2 RBS 3483961 3483965 . + 0 Rv0815c cysA2 May be a sulfotransferase involved in the formation of thiosulfate [catalytic activity: thiosulfate + cyanide = sulfite + thiocyanate]. Probable thiosulfate sulfurtransferase CysA2 (rhodanese-like protein) (thiosulfate cyanide transsulfurase) (thiosulfate thiotransferase) Rv0815c, (MTV043.07c, MT0837, O05793), len: 277 aa. Probable cysA2 (alternate gene name: sseC4), thiosulfate sulfurtransferase (see Wooff et al., 2002), equivalent to Q50036|CYSA|CYSA3|ML2198|THTR_MYCLE putative sulfurtransferase thiosulfate from Mycobacterium leprae (277 aa). Also highly similar to other putative thiosulfate sulfurtransferases e.g. P16385|THTR_SACER putative thiosulfate sulfurtransferase from Saccharopolyspora erythraea (Streptomyces erythraeus) (281 aa); NP_293941.1|NC_001263 thiosulfate sulfurtransferase from Deinococcus radiodurans (286 aa); etc. Identical second copy present as Rv3117|MTCY164.27|MT3199|O05793|cysA3 (277 aa) (100.0% identity in 277 aa overlap). Contains PS00683 Rhodanese C-terminal signature at C-terminus. Belongs to the rhodanese family. O05793 No intermediary metabolism and respiration ML2198 MMAR_4868 MSMEG_5789 Mb0838c NC_000962.3 Mycobrowser_v2 CDS 924951 925364 . + 0 Rv0832 PE_PGRS12 Function unknown PE-PGRS family protein PE_PGRS12 Rv0832, (MTV043.24), len: 137 aa. PE_PGRS12, Member of the Mycobacterium tuberculosis PE family, possibly PGRS subfamily of gly-rich proteins (see citation below), highly similar to many others e.g. MTCY1A11.25c|Z78020 (498 aa), FASTA scores: opt: 529, E(): 5.2e-22, (61.8% identity in 136 aa overlap); etc. Appears to have incurred frameshift as next ORF should be continuation; sequence has been checked but no error found. Q79FV8 No PE/PPE Mb0855 NC_000962.3 Mycobrowser_v2 CDS 911736 912683 . + 0 Rv0819 mshD Involved in the fourth step of mycothiol biosynthesis GCN5-related N-acetyltransferase, MshD Rv0819, (MTV043.11), len: 315 aa. MshD, acetyltransferase involved in mycothiol synthesis (see Koledin et al., 2002). Contains two GNAT (Gcn5-related N-acetyltransferase) domains. See Vetting et al. 2003, 2005, 2006. Equivalent to U2266N|U15182|MLU15182_24 hypothetical protein from Mycobacterium leprae (312 aa), FASTA scores: opt: 1540, E(): 0, (75.2% identity in 314 aa overlap). Also highly similar to CAB88484.1|AL353816 putative acetyltransferase from Streptomyces coelicolor (309 aa). O53831 No intermediary metabolism and respiration ML2193 MMAR_4864 Mb0842 NC_000962.3 Mycobrowser_v2 CDS 919634 920689 . + 0 Rv0826 Rv0826 Function unknown Conserved hypothetical protein Rv0826, (MTV043.18), len: 351 aa. Conserved hypothetical protein, similar to CAB94053.1|AL358672|SC7A12.06 hypothetical protein from Streptomyces coelicolor (300 aa); and NP_421372.1|NC_002696 hypothetical protein from Caulobacter crescentus (299 aa). Also similar to other proteins from Mycobacterium tuberculosis e.g. Rv1645c|Z85982|MTCY06H11.09 (351 aa), FASTA scores: opt: 1199, E(): 0, (57.5% identity in 299 aa overlap); Rv2237; Rv0276; etc. O53837 No conserved hypotheticals ML2183 MMAR_4854 MSMEG_5767 Mb0849 NC_000962.3 Mycobrowser_v2 CDS 920741 921133 . - 0 Rv0827c kmtR Represses transcription from the KMTR operator-promoter. Repression is alleviated by NI(II) or cobalt(II). Metal sensor transcriptional regulator KmtR (ArsR-SmtB family) Rv0827c, (MTV043.19c), len: 130 aa. KmtR, transcriptional regulator (See Campbell et al., 2007), similar to many e.g. CAC42856.1|AL592292 putative regulatory protein from Streptomyces coelicolor (115 aa); NP_301626.1|NC_002677 putative ArsR-family transcriptional regulator from Mycobacterium leprae (140 aa); BSUB0011_75|O31844|Z99114 YOZA protein from Bacillus subtilis (107 aa), FASTA scores: opt: 208, E(): 3.2e-08, (35.5% identity in 93 aa overlap); etc. Also similar to MTCY27.22c|Z95208 from Mycobacterium tuberculosis (135 aa), FASTA scores: opt: 201, E(): 1.2e-07, (35.7% identity in 98 aa overlap). Contains probable helix-turn helix motif from aa 42-63 (Score 1300, +3.61 SD). Belongs to the ArsR family of transcriptional regulators. O53838 No regulatory proteins ML2182 Mb0850c NC_000962.3 Mycobrowser_v2 RBS 912716 912720 . + 0 Rv0820 phoT Involved in active transport of inorganic phosphate across the membrane (import); responsible for energy coupling to the transport system. This is one of the proteins required for binding-protein-mediated phosphate transport. Probable phosphate-transport ATP-binding protein ABC transporter PhoT Rv0820, (MTV043.12), len: 258 aa. Probable phoT, phosphate-transport ATP-binding protein ABC transporter (see citation below), equivalent to PhoT|MLU15182_28|U15182 phosphate transport system ABC transporter from Mycobacterium leprae (258 aa), FASTA scores: opt: 1556, E(): 0, (91.5% identity in 258 aa overlap). Also highly similar to others e.g. CAB88472.1|AL353816 phosphate ABC transport system ATP-binding protein from Streptomyces coelicolor (258 aa); etc. Note that also highly similar to many PstB proteins e.g. AAC15686.1|AF045938|PstB putative ABC transporter nucleotide binding subunit from Mycobacterium smegmatis (258 aa). Contains PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters). O53832 No cell wall and cell processes ML2189 MMAR_4860 MSMEG_5779 Mb0843 NC_000962.3 Mycobrowser_v2 CDS 912726 913502 . + 0 Rv0820 phoT Involved in active transport of inorganic phosphate across the membrane (import); responsible for energy coupling to the transport system. This is one of the proteins required for binding-protein-mediated phosphate transport. Probable phosphate-transport ATP-binding protein ABC transporter PhoT Rv0820, (MTV043.12), len: 258 aa. Probable phoT, phosphate-transport ATP-binding protein ABC transporter (see citation below), equivalent to PhoT|MLU15182_28|U15182 phosphate transport system ABC transporter from Mycobacterium leprae (258 aa), FASTA scores: opt: 1556, E(): 0, (91.5% identity in 258 aa overlap). Also highly similar to others e.g. CAB88472.1|AL353816 phosphate ABC transport system ATP-binding protein from Streptomyces coelicolor (258 aa); etc. Note that also highly similar to many PstB proteins e.g. AAC15686.1|AF045938|PstB putative ABC transporter nucleotide binding subunit from Mycobacterium smegmatis (258 aa). Contains PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters). O53832 No cell wall and cell processes ML2189 MMAR_4860 MSMEG_5779 Mb0843 NC_000962.3 Mycobrowser_v2 CDS 1184883 1185740 . + 0 Rv1062 Rv1062 Function unknown Conserved hypothetical protein Rv1062, (MTV017.15), len: 285 aa. Conserved hypothetical protein, some similarity to AL079356|SC6G9_10 hypothetical protein in Streptomyces coelicolor (289 aa), FASTA scores: opt: 556, E(): 1.2e-27, (39.0% identity in 287 aa overlap), and Z99111|BSUB0008_176 Bacillus subtilis (260aa), FASTA scores: opt: 163, E(): 0.0013, (27.4% identity in 179aa overlap). O53410 No conserved hypotheticals MMAR_4404 MSMEG_5285 Mb1091 NC_000962.3 Mycobrowser_v2 CDS 913558 914199 . - 0 Rv0821c phoY2 Involved in transcriptional regulation of active transport of inorganic phosphate across the membrane. Probable phosphate-transport system transcriptional regulatory protein PhoY2 Rv0821c, (MTV043.13c), len: 213 aa. Probable phoY2, phosphate-transport system regulatory protein, highly similar to PhoY|MLU15182_29|U15182 phosphate transport system regulator from Mycobacterium leprae (222 aa), FASTA scores: opt: 1268, E(): 0, (93.0% identity in 213 aa overlap). Also similar to others e.g. NP_384620.1|NC_003047 probable phosphate transport system transcriptional regulator protein from Sinorhizobium meliloti (237 aa); etc. Also highly similar to MTCI418A.03c|Z96070|PhoY1 probable phosphate transport system transcriptional regulator protein from Mycobacterium tuberculosis (221 aa), FASTA scores: opt: 937, E(): 0, (63.4% identity in 213 aa overlap). Belongs to the PhoU family. P65720 No cell wall and cell processes ML2188 MMAR_4859 MSMEG_5776 Mb0844c NC_000962.3 Mycobrowser_v2 RBS 3687587 3687590 . - 0 Rv0821c phoY2 Involved in transcriptional regulation of active transport of inorganic phosphate across the membrane. Probable phosphate-transport system transcriptional regulatory protein PhoY2 Rv0821c, (MTV043.13c), len: 213 aa. Probable phoY2, phosphate-transport system regulatory protein, highly similar to PhoY|MLU15182_29|U15182 phosphate transport system regulator from Mycobacterium leprae (222 aa), FASTA scores: opt: 1268, E(): 0, (93.0% identity in 213 aa overlap). Also similar to others e.g. NP_384620.1|NC_003047 probable phosphate transport system transcriptional regulator protein from Sinorhizobium meliloti (237 aa); etc. Also highly similar to MTCI418A.03c|Z96070|PhoY1 probable phosphate transport system transcriptional regulator protein from Mycobacterium tuberculosis (221 aa), FASTA scores: opt: 937, E(): 0, (63.4% identity in 213 aa overlap). Belongs to the PhoU family. P65720 No cell wall and cell processes ML2188 MMAR_4859 MSMEG_5776 Mb0844c NC_000962.3 Mycobrowser_v2 CDS 914257 916311 . - 0 Rv0822c Rv0822c Function unknown Conserved protein Rv0822c, (MTV043.14c), len: 684 aa. Conserved protein, highly similar in the region between aa 370 - 580 to U2266O|U15182|MLU15182_30 hypothetical protein from Mycobacterium leprae (222 aa), FASTA scores: opt: 819, E(): 0, (60.6% identity in 221 aa overlap). More extended similarity to Rv3267|Z92771|MTCY71_7 from Mycobacterium tuberculosis (498 aa), FASTA scores: opt: 434, E(): 2.2e-17, (26.6% identity in 541 aa overlap), and Rv3484. Also similar to various proteins, preferiously putative membrane proteins and membrane-bound regulatory proteins e.g. CAC44512.1|AL596138 putative membrane protein from Streptomyces coelicolor (524 aa); U56901|BSU56901_1 regulatory protein from Bacillus subtilis (391 aa), FASTA scores: opt: 225, E(): 1.3e-05, (24.7% identity in 340 aa overlap). Contains hydrophobic stretch (aa ~ 160-195) and PS00041 Bacterial regulatory proteins, araC family signature. O53834 No conserved hypotheticals ML2187 MMAR_4858 MSMEG_5775 Mb0845c NC_000962.3 Mycobrowser_v2 RBS 916322 916326 . - 0 Rv0822c Rv0822c Function unknown Conserved protein Rv0822c, (MTV043.14c), len: 684 aa. Conserved protein, highly similar in the region between aa 370 - 580 to U2266O|U15182|MLU15182_30 hypothetical protein from Mycobacterium leprae (222 aa), FASTA scores: opt: 819, E(): 0, (60.6% identity in 221 aa overlap). More extended similarity to Rv3267|Z92771|MTCY71_7 from Mycobacterium tuberculosis (498 aa), FASTA scores: opt: 434, E(): 2.2e-17, (26.6% identity in 541 aa overlap), and Rv3484. Also similar to various proteins, preferiously putative membrane proteins and membrane-bound regulatory proteins e.g. CAC44512.1|AL596138 putative membrane protein from Streptomyces coelicolor (524 aa); U56901|BSU56901_1 regulatory protein from Bacillus subtilis (391 aa), FASTA scores: opt: 225, E(): 1.3e-05, (24.7% identity in 340 aa overlap). Contains hydrophobic stretch (aa ~ 160-195) and PS00041 Bacterial regulatory proteins, araC family signature. O53834 No conserved hypotheticals ML2187 MMAR_4858 MSMEG_5775 Mb0845c NC_000962.3 Mycobrowser_v2 CDS 921191 921613 . - 0 Rv0828c Rv0828c Function unknown; probably involved in deamination of specific substrate. Possible deaminase Rv0828c, (MTV043.20c), len: 140 aa. Possible deaminase, with its N-terminus highly similar to middle part of NP_302602.1|NC_002677 possible cytidine/deoxycytidylate deaminase from Mycobacterium leprae (171 aa). Also similar to other deaminases e.g. CAC18715.2|AL451182 putative deaminase from Streptomyces coelicolor (167 aa); NP_251189.1|NC_002516 probable deaminase from Pseudomonas aeruginosa (151 aa); NP_108387.1|NC_002678 nitrogen fixation protein gene from Mesorhizobium loti (149 aa); etc. Also similar to many conserved hypothetical proteins e.g. NP_389200.1|NC_000964 hypothetical protein from Bacillus subtilis (156 aa), FASTA scores: E(): 1.3e-07, (38.9% identity in 95 aa overlap); etc. And similar to Rv3752c possible deaminase from Mycobacterium tuberculosis. Contains PS00903 Cytidine and deoxycytidylate deaminases zinc-binding region signature. Belongs to the cytidine and deoxycytidylate deaminases family. O53839 No intermediary metabolism and respiration MMAR_4851 Mb0851c NC_000962.3 Mycobrowser_v2 CDS 921575 921865 . + 0 Rv0829 Rv0829 Required for the transposition of the insertion element IS1605'. Possible transposase (fragment) Rv0829, (MTV043.21), len: 96 aa. Possible transposase for IS1605' (fragment), similar to C-terminal end of many mycobacterial transposases and hypothetical proteins e.g. Z74024|MTCY274_16 from Mycobacterium tuberculosis (460 aa), FASTA scores: opt: 668, E(): 6.2e-32, (98.9% identity in 93 aa overlap); MTV002_57|O33333 transposase from Mycobacterium tuberculosis ; L07627|SERRY1_1 insertion element IS1136 from Saccharopolyspora erythraea (90 aa), FASTA score: (34.9% identity in 83 aa overlap). O53840 No insertion seqs and phages Mb0852 NC_000962.3 Mycobrowser_v2 RBS 921959 921964 . + 0 Rv0830 Rv0830 Possible methyltransferase Possible S-adenosylmethionine-dependent methyltransferase Rv0830, (MTV043.22), len: 301 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), member of Mycobacterium tuberculosis protein family consisting of the proteins Rv0726c, Rv0731c, Rv3399, Rv1729c|Z81360|MTCY4C12_14c (312 aa), FASTA scores: opt: 1014, E(): 0, (54.1% identity in 292 aa overlap); etc. O53841 No lipid metabolism ML2181 MMAR_4850 Mb0853 NC_000962.3 Mycobrowser_v2 CDS 921970 922875 . + 0 Rv0830 Rv0830 Possible methyltransferase Possible S-adenosylmethionine-dependent methyltransferase Rv0830, (MTV043.22), len: 301 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), member of Mycobacterium tuberculosis protein family consisting of the proteins Rv0726c, Rv0731c, Rv3399, Rv1729c|Z81360|MTCY4C12_14c (312 aa), FASTA scores: opt: 1014, E(): 0, (54.1% identity in 292 aa overlap); etc. O53841 No lipid metabolism ML2181 MMAR_4850 Mb0853 NC_000962.3 Mycobrowser_v2 CDS 925361 927610 . + 0 Rv0833 PE_PGRS13 Function unknown PE-PGRS family protein PE_PGRS13 Rv0833, (MTV043.25), len: 749 aa. PE_PGRS13, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), but lacking N-terminal domain (present in preceding ORF), possibly due to frameshift. Similar in part to many others e.g. MTCY28_25|Z95890 (914 aa), FASTA scores: opt: 2726, E(): 0, (60.1% identity in 776 aa overlap); etc. Q79FV7 No PE/PPE Mb0856 NC_000962.3 Mycobrowser_v2 CDS 927837 930485 . - 0 Rv0834c PE_PGRS14 Function unknown PE-PGRS family protein PE_PGRS14 Rv0834c, (MTV043.26c), len: 882 aa. PE_PGRS14, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002), highly similar to many others e.g. MTCY493_4|Z95844 (1329 aa), FASTA scores: opt: 2577, E(): 0, (52.0% identity in 950 aa overlap); etc. Thought to be differentially expressed within host cells (see Triccas et al., 1999). Q79FV6 No PE/PPE MMAR_0492 Mb0857c NC_000962.3 Mycobrowser_v2 CDS 934720 935490 . + 0 Rv0838 lpqR Unknown Probable conserved lipoprotein LpqR Rv0838, (MTV043.31), len: 256 aa. Probable lpqR, conserved lipoprotein. Similar (except in N-terminus) to hypothetical proteins and D-alanyl-D-alanine dipeptidases e.g. NP_416005.1|NC_000913 hypothetical protein from Escherichia coli strain K12 (193 aa); NP_421076.1|NC_002696 D-alanyl-D-alanine dipeptidase from Caulobacter crescentus (212 aa); Q06241|VANX_ENTFC D-alanyl-D-alanine dipeptidase from Enterococcus faecium (202 aa), FASTA scores: opt: 198, E(): 1.9e-05, (28.1% identity in 199 aa overlap); etc. Contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O53850 No cell wall and cell processes MMAR_4800 MSMEG_5879 Mb0861 NC_000962.3 Mycobrowser_v2 CDS 935577 936389 . + 0 Rv0839 Rv0839 Function unknown Conserved hypothetical protein Rv0839, (MTV043.32), len: 270 aa. Conserved hypothetical protein, similar to various hypothetical proteins or methyltransferases from yeast and bacteria e.g. T34740|SC1E6.19c|AL033505|SC1E6_19 hypothetical protein from Streptomyces coelicolor (273 aa), FASTA scores: opt: 1102, E(): 0, (58.6% identity in 263 aa overlap); T38024|Z98598|SPAC1B3.06c hypothetical protein from Schizosaccharomyces pombe (278 aa), FASTA scores: opt: 562, E(): 1.9e-3, (36.4% identity in 269 aa overlap); JC6531 avermectin B 5-O-methyltransferase from Streptomyces avermitilis (283 aa); etc. Also similar to other Mycobacterium tuberculosis hypothetical proteins that may be methyltransferases e.g. Rv1523, Rv2952, Rv1405c, etc. O53851 No conserved hypotheticals MMAR_4799 Mb0862 NC_000962.3 Mycobrowser_v2 CDS 936457 937317 . - 0 Rv0840c pip Specifically catalyzes the removal of N-terminal proline residues from peptides. Thought to release the N-terminal proline from the dipeptides, pro-pro, pro-GLN, pro-TRP and pro-TYR; also from amides (pro-beta NA) and oligopeptides, pro-LEU-GLYNH2, pro-LEU-GLY and pro-PHE-GLY-LYS. Higher activity toward small peptides (up to three residues), but very low activity for longer peptides [catalytic activity: release of a N-terminal proline from a peptide]. Probable proline iminopeptidase Pip (prolyl aminopeptidase) (pap) Rv0840c, (MTV043.33c), len: 286 aa. Possible pip, proline iminopeptidase, similar to many e.g. P46541|PIP_BACCO proline iminopeptidase from bacillus coagulans (288 aa), FASTA scores: opt: 657, E(): 0, (37.6% identity in 282 aa overlap); NP_386922.1|NC_003047 putative proline iminopeptidase protein from Sinorhizobium meliloti (296 aa); etc. Belongs to peptidase family S33. O53852 No intermediary metabolism and respiration MMAR_4789 MSMEG_2681 Mb0863c NC_000962.3 Mycobrowser_v2 RBS 937322 937326 . - 0 Rv0840c pip Specifically catalyzes the removal of N-terminal proline residues from peptides. Thought to release the N-terminal proline from the dipeptides, pro-pro, pro-GLN, pro-TRP and pro-TYR; also from amides (pro-beta NA) and oligopeptides, pro-LEU-GLYNH2, pro-LEU-GLY and pro-PHE-GLY-LYS. Higher activity toward small peptides (up to three residues), but very low activity for longer peptides [catalytic activity: release of a N-terminal proline from a peptide]. Probable proline iminopeptidase Pip (prolyl aminopeptidase) (pap) Rv0840c, (MTV043.33c), len: 286 aa. Possible pip, proline iminopeptidase, similar to many e.g. P46541|PIP_BACCO proline iminopeptidase from bacillus coagulans (288 aa), FASTA scores: opt: 657, E(): 0, (37.6% identity in 282 aa overlap); NP_386922.1|NC_003047 putative proline iminopeptidase protein from Sinorhizobium meliloti (296 aa); etc. Belongs to peptidase family S33. O53852 No intermediary metabolism and respiration MMAR_4789 MSMEG_2681 Mb0863c NC_000962.3 Mycobrowser_v2 CDS 937593 937835 . + 0 Rv0841 Rv0841 Unknown Probable conserved transmembrane protein Rv0841, len: 80 aa. Conserved transmembrane protein, highly similar to C-terminus of next ORF Rv0842|O53854 putative membrane protein from Mycobacterium tuberculosis (442 aa), FASTA scores: opt: 246, E(): 3.3e-10, (59.7% identity in 72 aa overlap). Replace previous Rv0841c. Q8VKC9 No cell wall and cell processes Mb0864c NC_000962.3 Mycobrowser_v2 CDS 1308223 1308792 . - 0 Rv1176c Rv1176c Function unknown Conserved hypothetical protein Rv1176c, (MTV005.12c), len: 189 aa. Conserved hypothetical protein, some similarity to P94443|D78508 hypothetical protein from Bacillus subtilis (182 aa), FASTA scores: opt: 219, E(): 1.7e-15, (25.1% identity in 183 aa overlap). Similar to Mycobacterium tuberculosis hypothetical protein Rv0047c. O50432 No conserved hypotheticals Mb1209c NC_000962.3 Mycobrowser_v2 RBS 938103 938107 . + 0 Rv0842 Rv0842 Unknown Probable conserved integral membrane protein Rv0842, (MT0864, MTV043.35), len: 430 aa. Probable conserved integral membrane protein, showing similarity with other integral membrane proteins e.g. P28246|BCR_ECOLI bicyclomycin resistance protein from EScherichia coli (396 aa), FASTA scores: opt: 216, E(): 5.4e-07, (23.7% identity in 376 aa overlap); etc. O53854 No cell wall and cell processes MMAR_4785 Mb0865 NC_000962.3 Mycobrowser_v2 CDS 938112 939404 . + 0 Rv0842 Rv0842 Unknown Probable conserved integral membrane protein Rv0842, (MT0864, MTV043.35), len: 430 aa. Probable conserved integral membrane protein, showing similarity with other integral membrane proteins e.g. P28246|BCR_ECOLI bicyclomycin resistance protein from EScherichia coli (396 aa), FASTA scores: opt: 216, E(): 5.4e-07, (23.7% identity in 376 aa overlap); etc. O53854 No cell wall and cell processes MMAR_4785 Mb0865 NC_000962.3 Mycobrowser_v2 RBS 939375 939379 . + 0 Rv0843 Rv0843 Function unknown; probably involved in cellular metabolism. Probable dehydrogenase Rv0843, (MTV043.36), len: 334 aa. Probable dehydrogenase, similar to various dehydrogenases e.g. Q46142|Q46142 TPP-dependent acetoin dehydrogenase (326 aa), FASTA scores: opt: 500, E(): 2.4e-26, (32.3% identity in 300 aa overlap); P51267|ODPA_PORPU pyruvate dehydrogenase E1 component from Porphyra purpurea (344 aa), FASTA scores: opt: 451, E(): 4.7e-23, (29.6% identity in 311 aa overlap); etc. Also similar to Rv2497c|pdhA pyruvate dehydrogenase E1 component from Mycobacterium tuberculosis (367 aa). O53855 No intermediary metabolism and respiration MMAR_4784 Mb0866 NC_000962.3 Mycobrowser_v2 CDS 939388 940392 . + 0 Rv0843 Rv0843 Function unknown; probably involved in cellular metabolism. Probable dehydrogenase Rv0843, (MTV043.36), len: 334 aa. Probable dehydrogenase, similar to various dehydrogenases e.g. Q46142|Q46142 TPP-dependent acetoin dehydrogenase (326 aa), FASTA scores: opt: 500, E(): 2.4e-26, (32.3% identity in 300 aa overlap); P51267|ODPA_PORPU pyruvate dehydrogenase E1 component from Porphyra purpurea (344 aa), FASTA scores: opt: 451, E(): 4.7e-23, (29.6% identity in 311 aa overlap); etc. Also similar to Rv2497c|pdhA pyruvate dehydrogenase E1 component from Mycobacterium tuberculosis (367 aa). O53855 No intermediary metabolism and respiration MMAR_4784 Mb0866 NC_000962.3 Mycobrowser_v2 CDS 1008944 1010062 . + 0 Rv0906 Rv0906 Function unknown Conserved protein Rv0906, (MTCY31.34), len: 372 aa. Conserved protein, highly similar to others e.g. SC6A5.25|AL049485|T35416 hypothetical protein from Streptomyces coelicolor (370 aa), FASTA scores: opt: 1125, E(): 0, (51.3% identity in 335 aa overlap); NP_242955.1|NC_002570|BH2089 conserved protein from Bacillus halodurans (370 aa); etc. Also shows some similarity to C-terminus of Q48412|ROMA_KLEPN Q48412 outer membrane protein roma (fragment) from Klebsiella pneumoniae (132 aa), FASTA scores: opt: 319, E(): 8.5e-14, (46.2% identity in 104 aa overlap); NP_105215.1|NC_002678 hypothetical protein which contains similarity to outer membrane protein romA from Enterobacter cloacae (350 aa); etc. Predicted to be an outer membrane protein (See Song et al., 2008). P64759 No conserved hypotheticals ML2117 MMAR_4624 MSMEG_5638 Mb0930 NC_000962.3 Mycobrowser_v2 CDS 951183 951626 . + 0 Rv0854 Rv0854 Function unknown Conserved protein Rv0854, (MTV043.47), len: 147 aa. Conserved protein, similar to several hypothetical protein from Mycobacterium leprae e.g. NP_301674.1|NC_002677 (144 aa); NP_302683.1|NC_002677|Z95398|MLCL622.27c (156 aa), FASTA scores: opt: 193, E(): 1.6e-06, (24.6% identity in 134 aa overlap); NP_301218.1|NC_002677 (146 aa); MTCI28.04|Z97050 (184 aa), FASTA scores: opt: 171, E(): 5.8e-05, (21.5% identity in 135 aa overlap). Also similar to SC6G10.02c|T35511|AL049497|SC6G10_2 hypothetical protein from Streptomyces coelicolor (144 aa), FASTA scores: opt: 344, E(): 6.1e- 17, (37.6% identity in 141 aa overlap). And similar to many proteins from Mycobacterium tuberculosis e.g. downstreams ORFs Rv0856 and Rv0857, etc. O53866 No conserved hypotheticals ML2166 MMAR_4682 MSMEG_5736 Mb0877 NC_000962.3 Mycobrowser_v2 CDS 940456 941106 . - 0 Rv0844c narL Involved in transcriptional mechanism and regulates nitrate/nitrite. Possible nitrate/nitrite response transcriptional regulatory protein NarL Rv0844c, (MTV043.37c), len: 216 aa. Possible narL, nitrate/nitrite response regulator protein, similar to many e.g. CAB44989.1|AJ131854 NarL protein from Pseudomonas stutzeri (218 aa); CAA75536.1|Y15252 nitrate/nitrite regulatory protein from Pseudomonas aeruginosa (216 aa); PCC6803|D64005|SYCSLRG_24 NarL protein from Synechocystis sp. (209 aa), FASTA scores: opt: 438, E(): 1.5e-23, (34.6% identity in 208 aa overlap); etc. Also similar to unidentified regulator e.g. CAB76009.1|AL157916 putative two-component system response regulator from Streptomyces coelicolor (224 aa); etc. Contains probable helix-turn helix motif from aa 170-191 (Score 1124, +3.02 SD). O53856 No regulatory proteins MMAR_4782 MSMEG_0105 Mb0867c NC_000962.3 Mycobrowser_v2 CDS 941190 942467 . + 0 Rv0845 Rv0845 Possible sensor part of a two component regulatory system. Possible two component sensor kinase Rv0845, (MTV043.38), len: 425 aa. Possible two-component sensor kinase, with its C-terminus similar to C-terminal part of others e.g. NP_294951.1|NC_001263 two-component sensor histidine kinase from Deinococcus radiodurans (469 aa); CAC32293.1|AL583943 putative two component system histidine kinase from Streptomyces coelicolor (404 aa); NP_464546.1|NC_003210 protein similar to two-component sensor histidine kinase from Listeria monocytogenes (352 aa); BSUB0017_193|Z9912 two-component sensor kinase from Bacillus subtilis (360 aa), FASTA scores: opt: 275, E(): 1.6e-11, (30.3% identity in 234 aa overlap); etc. O53857 No regulatory proteins MMAR_4781 MSMEG_0106 Mb0868 NC_000962.3 Mycobrowser_v2 CDS 942680 944194 . - 0 Rv0846c Rv0846c May have multicopper oxidase activity. Probable oxidase Rv0846c, (MTV043.39c), len: 504 aa. Probable oxidase, showing similarity with several oxidases, mainly L-ascorbate oxidases and copper resistance proteins a (precursors) e.g. P24792|ASO_CUCMA L-ascorbate oxidase precursor (ascorbase) from Cucurbita maxima (Pumpkin) (Winter squash) (579 aa), FASTA scores: opt: 423, E(): 5.8e-18, (28.4% identity in 493 aa overlap); AF010496|AF010496_32 potential multicopper oxidase from Rhodobacter capsulatus (491 aa), FASTA scores: opt: 490, E(): 2.7e-22, (28.8% identity in 510 aa overlap); 47452|PCOA_ECOLI copper resistance protein A precursor (belongs to the family of multicopper oxidases) from Escherichia coli strain K12 (605 aa); etc. Contains PS00080 Multicopper oxidases signature 2 at C-terminus. Seems to belong to the family of multicopper oxidases. O53858 No intermediary metabolism and respiration ML2171 MMAR_4770 Mb0869c NC_000962.3 Mycobrowser_v2 RBS 944202 944206 . - 0 Rv0846c Rv0846c May have multicopper oxidase activity. Probable oxidase Rv0846c, (MTV043.39c), len: 504 aa. Probable oxidase, showing similarity with several oxidases, mainly L-ascorbate oxidases and copper resistance proteins a (precursors) e.g. P24792|ASO_CUCMA L-ascorbate oxidase precursor (ascorbase) from Cucurbita maxima (Pumpkin) (Winter squash) (579 aa), FASTA scores: opt: 423, E(): 5.8e-18, (28.4% identity in 493 aa overlap); AF010496|AF010496_32 potential multicopper oxidase from Rhodobacter capsulatus (491 aa), FASTA scores: opt: 490, E(): 2.7e-22, (28.8% identity in 510 aa overlap); 47452|PCOA_ECOLI copper resistance protein A precursor (belongs to the family of multicopper oxidases) from Escherichia coli strain K12 (605 aa); etc. Contains PS00080 Multicopper oxidases signature 2 at C-terminus. Seems to belong to the family of multicopper oxidases. O53858 No intermediary metabolism and respiration ML2171 MMAR_4770 Mb0869c NC_000962.3 Mycobrowser_v2 RBS 944330 944334 . + 0 Rv0847 lpqS Unknown Probable lipoprotein LpqS Rv0847, (MTV043.40), len: 130 aa. Probable lpqS, lipoprotein. Contains possible signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O53859 No cell wall and cell processes MMAR_4769 Mb0870 NC_000962.3 Mycobrowser_v2 CDS 944343 944735 . + 0 Rv0847 lpqS Unknown Probable lipoprotein LpqS Rv0847, (MTV043.40), len: 130 aa. Probable lpqS, lipoprotein. Contains possible signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O53859 No cell wall and cell processes MMAR_4769 Mb0870 NC_000962.3 Mycobrowser_v2 CDS 944938 946056 . + 0 Rv0848 cysK2 Thought to be involved in cysteine biosynthesis [catalytic activity: O3-acetyl-L-serine + H(2)S = L-cysteine + acetate]. Possible cysteine synthase a CysK2 (O-acetylserine sulfhydrylase) (O-acetylserine (thiol)-lyase) (CSASE) Rv0848, (MTV043.41), len: 372 aa. Possible cysK2, cysteine synthase A, but could be also a cysteine synthase B cysM2-product, similar to many e.g. NP_109408.1|NC_002682 cysteine synthase from Mesorhizobium loti (357 aa); Q44004|CYSM_ALCEU cysteine synthase from Alcaligenes eutrophus strain CH34 (Ralstonia eutropha) (339 aa), FASTA scores: opt: 511, E(): 1.7e-25, (35.0% identity in 314 aa overlap); etc. Belongs to the cysteine synthase/cystathionine beta-synthase family. Cofactor: pyridoxal phosphate. Note that previously known as cysM3. Q79FV4 No intermediary metabolism and respiration MMAR_4768 Mb0871 NC_000962.3 Mycobrowser_v2 RBS 946043 946047 . + 0 Rv0849 Rv0849 Thought to be involved in transport of undetermined substrate (possibly drug) across the membrane. Responsible for the translocation of the substrate across the membrane. Probable conserved integral membrane transport protein Rv0849, (MTV043.42), len: 419 aa. Probable conserved integral membrane transport protein, possibly member of major facilitator superfamily (MFS) involved in transport of drug, showing similarity with others e.g. T35055 probable transport system permease protein from Streptomyces coelicolor (436 aa); NP_295031.1|NC_001263 major facilitator family protein from Deinococcus radiodurans (458 aa); NP_455659.1|NC_003198 putative membrane transporter from Salmonella enterica subsp. enterica serovar Typhi (402 aa); etc. O53861 No cell wall and cell processes MMAR_4767 Mb0872 NC_000962.3 Mycobrowser_v2 CDS 946056 947315 . + 0 Rv0849 Rv0849 Thought to be involved in transport of undetermined substrate (possibly drug) across the membrane. Responsible for the translocation of the substrate across the membrane. Probable conserved integral membrane transport protein Rv0849, (MTV043.42), len: 419 aa. Probable conserved integral membrane transport protein, possibly member of major facilitator superfamily (MFS) involved in transport of drug, showing similarity with others e.g. T35055 probable transport system permease protein from Streptomyces coelicolor (436 aa); NP_295031.1|NC_001263 major facilitator family protein from Deinococcus radiodurans (458 aa); NP_455659.1|NC_003198 putative membrane transporter from Salmonella enterica subsp. enterica serovar Typhi (402 aa); etc. O53861 No cell wall and cell processes MMAR_4767 Mb0872 NC_000962.3 Mycobrowser_v2 CDS 947312 947644 . + 0 Rv0850 Rv0850 Required for the transposition of an insertion element. Putative transposase (fragment) Rv0850, (MTV043.43), len: 110 aa. Putative transposase (fragment), similar in part to others e.g. Q45144|Q4514 transposable element IS31831 (436 aa), FASTA scores: opt: 175, E(): 4.3e-05, (38.6% identity in 57 aa overlap); etc. O53862 No insertion seqs and phages Mb0873 NC_000962.3 Mycobrowser_v2 CDS 947641 948468 . - 0 Rv0851c Rv0851c Function unknown; possibly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv0851c, (MTV043.44c), len: 275 aa. Probable short-chain dehydrogenase/reductase, similar to many e.g. Q01198|LIGD_PSEPA C alpha-dehydrogenase (SDR family) from Pseudomonas paucimobilis (Sphingomonas paucimobilis) (305 aa); D11473|PSELIG_1 C alpha-dehydrogenase from P. paucimobilis (305 aa), FASTA scores: opt: 468, E(): 4.9e-23, (30.8% identity in 279 aa overlap); NP_421969.1|NC_002696 short chain dehydrogenase family protein from Caulobacter crescentus (278 aa); etc. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. O53863 No intermediary metabolism and respiration MMAR_4755 Mb0874c NC_000962.3 Mycobrowser_v2 RBS 948478 948481 . - 0 Rv0851c Rv0851c Function unknown; possibly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv0851c, (MTV043.44c), len: 275 aa. Probable short-chain dehydrogenase/reductase, similar to many e.g. Q01198|LIGD_PSEPA C alpha-dehydrogenase (SDR family) from Pseudomonas paucimobilis (Sphingomonas paucimobilis) (305 aa); D11473|PSELIG_1 C alpha-dehydrogenase from P. paucimobilis (305 aa), FASTA scores: opt: 468, E(): 4.9e-23, (30.8% identity in 279 aa overlap); NP_421969.1|NC_002696 short chain dehydrogenase family protein from Caulobacter crescentus (278 aa); etc. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. O53863 No intermediary metabolism and respiration MMAR_4755 Mb0874c NC_000962.3 Mycobrowser_v2 CDS 972546 973706 . - 0 Rv0874c Rv0874c Function unknown Conserved hypothetical protein Rv0874c, (MTCY31.02c), len: 386 aa. Conserved hypothetical protein, highly similar in part to SPU62616_1 hypothetical protein from Synechococcus sp. (280 aa), FASTA scores: E(): 6.3e-26, (35.2% identity in 264 aa overlap); SYCSLLLH_102 from Synechocystis sp. (447 aa), FASTA scores: E(): 1.1e-18, (29.5% identity in 400 aa overlap). Also highly similar to Rv0628c|MTCY20H10_9 from Mycobacterium tuberculosis (383 aa), FASTA scores: E():0, (81.5% identity in 383 aa overlap). P0A5D3 No conserved hypotheticals Mb0898c NC_000962.3 Mycobrowser_v2 CDS 948559 949395 . + 0 Rv0852 fadD16 Function unknown, but involved in lipid degradation. Possible fatty-acid-CoA ligase FadD16 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv0852, (MTV043.45), len: 278 aa. Possible fadD16, fatty-acid-CoA synthetase, similar in part to various CoA ligases e.g. P18163|LCFB_RAT long-chain-fatty-acid--CoA ligase from Rattus norvegicus (Rat) (699 aa); D49366|LEP4CCOALA_1 4-coumarate:CoA ligase from Lithospermum erythrorhizon (636 aa), FASTA scores: opt: 134, E(): 0.15, (26.8% identity in 213 aa overlap); orgp|L09229|HUMFACAL_1 long-chain acyl-coenzyme A from homo sapiens (human) (699 aa), FASTA score: (50.0% identity in 40 aa overlap); etc. Contains PS00626 Regulator of chromosome condensation (RCC1) signature 2. O53864 No lipid metabolism ML2168 MMAR_4685 MSMEG_3566 Mb0875 NC_000962.3 Mycobrowser_v2 CDS 949436 951118 . - 0 Rv0853c pdc Possible indole-3-pyruvate decarboxylase; EC 4.1.1.74 [catalytic activity: 3-(indol-3-YL)pyruvate = 2-(indol-3-YL)acetaldehyde + CO2], or possible pyruvate decarboxylase; EC 4.1.1.1 [catalytic activity: a 2-oxo acid = an aldehyde + CO2]. Probable pyruvate or indole-3-pyruvate decarboxylase Pdc Rv0853c, (MTV043.46c), len: 560 aa. Probable pdc, pyruvate or indole-pyruvate decarboxylase, equivalent to NP_302424.1|NC_002677 pyruvate (or indolepyruvate) decarboxylase from Mycobacterium leprae (569 aa). Also highly similar to others e.g. AAB06571.1|L80006 indolepyruvate decarboxylase from Pantoea agglomerans (550 aa); Q12629|DCPY_KLULA pyruvate decarboxylase from Kluyveromyces marxianus var. lactis (563 aa); P71323 indolepyruvate decarboxylase from Enterobacter herbicola (550 aa), FASTA scores: opt: 1642, E(): 0, (48.1% identity in 547 aa overlap); P23234|DCIP_ENTCL indole-3-pyruvate decarboxylase (indolepyruvate decarboxylase) from Enterobacter cloacae (552 aa), FASTA scores: opt: 1596, E(): 0, (46.8% identity in 551 aa overlap); etc. Contains PS00187 Thiamine pyrophosphate enzymes signature and PS00017 ATP/GTP-binding site motif A (P-loop). Cofactor: thiamine pyrophosphate. O53865 No intermediary metabolism and respiration ML2167 MMAR_4683 MSMEG_5735 Mb0876c NC_000962.3 Mycobrowser_v2 CDS 951632 952711 . + 0 Rv0855 far Function unknown, but involvement in lipid degradation (racemization). Probable fatty-acid-CoA racemase Far Rv0855, (MTV043.48), len: 359 aa. Probable far, fatty acid-CoA racemase, highly similar to CAB08122.1|Z94723 unknown protein from Mycobacterium leprae (253 aa) (C-terminus shorter). Also similar to many eukaryotic and bacteria racemases e.g. T35425 probable fatty acid CoA racemase from Streptomyces coelicolor (387 aa); P70473|AMAC_RAT alpha-methylacyl-CoA racemase (2-methylacyl-CoA racemase) (2-arylpropionyl-CoA epimerase) from Rattus norvegicus (Rat) (382 aa); NP_103687.1|NC_002678 probable fatty acid Co-a racemase from Mesorhizobium loti (389 aa); etc. Also similar to proteins from Mycobacterium tuberculosis e.g. Rv1143|MTCI65.10|MCR from Mycobacterium tuberculosis (360 aa), FASTA scores: opt: 1373, E(): 0, (56.8% identity in 359 aa overlap), Rv1866|MTCY359.07 (C-terminal half) (778 aa), Rv3272 (360 aa). O53867 No lipid metabolism ML2165 MMAR_4681 MSMEG_5734 Mb0878 NC_000962.3 Mycobrowser_v2 RBS 952816 952819 . + 0 Rv0856 Rv0856 Function unknown Conserved hypothetical protein Rv0856, (MTV043.49), len: 134 aa. Conserved hypothetical protein, showing weak similarity with NP_301674.1| (NC_002677) conserved hypothetical protein from Mycobacterium leprae (144 aa); and SC6G10.02c|T35511 hypothetical protein from Streptomyces coelicolor (144 aa). Also highly similar to other proteins from Mycobacterium tuberculosis e.g. neighbouring ORF downstream Rv0857 conserved hypothetical protein (126 aa), FASTA scores: E(): 7.4e-27, (62.0% identity in 100 aa overlap); neighbouring ORF Rv0854|MTV043_47 conserved hypothetical protein (147 aa), FASTA scores: E(): 1.6e-15, (36.6% identity in 123 aa overlap), MTCI28.04|Z97050|MTCI28_4 (184 aa), FASTA scores: opt: 127, E(): 0.036, (26.0% identity in 127 aa overlap); and MLCL622.27c|Z95398 (156 aa), FASTA scores: opt: 123, E(): 0.06, (26.4% identity in 125 aa overlap). O53868 No conserved hypotheticals Mb0879 NC_000962.3 Mycobrowser_v2 CDS 952825 953229 . + 0 Rv0856 Rv0856 Function unknown Conserved hypothetical protein Rv0856, (MTV043.49), len: 134 aa. Conserved hypothetical protein, showing weak similarity with NP_301674.1| (NC_002677) conserved hypothetical protein from Mycobacterium leprae (144 aa); and SC6G10.02c|T35511 hypothetical protein from Streptomyces coelicolor (144 aa). Also highly similar to other proteins from Mycobacterium tuberculosis e.g. neighbouring ORF downstream Rv0857 conserved hypothetical protein (126 aa), FASTA scores: E(): 7.4e-27, (62.0% identity in 100 aa overlap); neighbouring ORF Rv0854|MTV043_47 conserved hypothetical protein (147 aa), FASTA scores: E(): 1.6e-15, (36.6% identity in 123 aa overlap), MTCI28.04|Z97050|MTCI28_4 (184 aa), FASTA scores: opt: 127, E(): 0.036, (26.0% identity in 127 aa overlap); and MLCL622.27c|Z95398 (156 aa), FASTA scores: opt: 123, E(): 0.06, (26.4% identity in 125 aa overlap). O53868 No conserved hypotheticals Mb0879 NC_000962.3 Mycobrowser_v2 CDS 1417658 1418356 . - 0 Rv1268c Rv1268c Unknown Hypothetical protein Rv1268c, (MTCY50.14), len: 232 aa. Hypothetical unknown protein, probably secreted protein : contains possible signal peptide sequence (score 7.9 at residue 28). Predicted to be an outer membrane protein (See Song et al., 2008). P64791 No conserved hypotheticals MMAR_4154 Mb1299c NC_000962.3 Mycobrowser_v2 RBS 1418363 1418368 . - 0 Rv1268c Rv1268c Unknown Hypothetical protein Rv1268c, (MTCY50.14), len: 232 aa. Hypothetical unknown protein, probably secreted protein : contains possible signal peptide sequence (score 7.9 at residue 28). Predicted to be an outer membrane protein (See Song et al., 2008). P64791 No conserved hypotheticals MMAR_4154 Mb1299c NC_000962.3 Mycobrowser_v2 CDS 953257 953730 . + 0 Rv0857 Rv0857 Function unknown Conserved hypothetical protein Rv0857, (MTV043.50), len: 157 aa. Conserved hypothetical protein, showing weak similarity with Q9X7Y8|SC6G10.02c|T35511 hypothetical protein from Streptomyces coelicolor (144 aa), FASTA scores: opt: 215, E(): 7.6e-08, (30.282% identity in 142 aa overlap). Also highly similar to other proteins from Mycobacterium tuberculosis e.g. upstream ORF Rv0856 (134 aa), FASTA scores: opt: 566, E(): 2e-32, (58.15% identity in 129 aa overlap); upstream ORF Rv0854 (147 aa), FASTA scores: opt: 401, E(): 7.2e-21, (41.8% identity in 146 aa overlap); MTCI28.04|Z97050 (184 aa), FASTA scores: opt: 122, E(): 0.031, (29.4% identity in 85 aa overlap); and MLCL622.27c|Z95398 (156 aa), FASTA scores: opt: 114, E(): 0.1, (30.9% identity in 55 aa overlap). Length extended since first submission (+33 aa). O53869 No conserved hypotheticals ML2164 MMAR_4679 Mb0880 NC_000962.3 Mycobrowser_v2 CDS 953727 954920 . - 0 Rv0858c dapC Involved in biosynthesis of diaminopimelate and lysine from aspartate semialdehyde (at the fourth step) [catalytic activity: L-glutamate + N-succinyl-2-amino-6-ketopimelate = 2-oxoglutarate + N-succinyl-L,L-2,6-diaminopimelate Probable N-succinyldiaminopimelate aminotransferase DapC (DAP-at) Rv0858c, (MTV043.51c), len: 397 aa. Probable dapC, N-succinyldiaminopimelate aminotransferase, highly similar to others from Eukaryota and bacteria, especially aspartate aminotransferases (transaminases), e.g. NP_177890.1|NC_003070 putative aminotransferase from Arabidopsis thaliana (440 aa); NP_419555.1|NC_002696 aminotransferase class I from Caulobacter crescentus (385 aa); NP_415133.1|NC_000913|AE0001|ECAE000165_8 putative aminotransferase from Escherichia coli strain K12 (386 aa), FASTA scores: opt: 830, E(): 0, (38.0% identity in 389 aa overlap); X99521|TAX99521_1 aspartate aminotransferase from Thermus aquaticus (383 aa), FASTA scores: opt: 702, E(): 0, (34.9% identity in 393 aa overlap); etc. Also similar to other putative aminotransferases from Mycobacterium tuberculosis e.g. Rv2294, Rv3565, etc. O53870 No intermediary metabolism and respiration ML2163 MMAR_4678 MSMEG_5725 Mb0881c NC_000962.3 Mycobrowser_v2 CDS 1024684 1025184 . + 0 Rv0919 Rv0919 Acetylation, substrate unknown GCN5-related N-acetyltransferase Rv0919, (MTCY21C12.13), len: 166 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Some similarity to Q50115 hypothetical protein from Mycobacterium leprae (90 aa), FASTA scores: opt: 243, E(): 5.3e-11, (56.5% identity in 85 aa overlap). Alternative nucleotide at position 1025106 (T->C; F141F) has been observed. O05911 No intermediary metabolism and respiration ML2107 Mb0943 NC_000962.3 Mycobrowser_v2 RBS 955066 955070 . + 0 Rv0859 fadA Function unknown, but involvement in lipid degradation. Possible acyl-CoA thiolase FadA Rv0859, (MTV043.52), len: 403 aa. Possible fadA, acyl-CoA thiolase, equivalent to NP_302423.1|NC_002677 putative beta-ketoadipyl CoA thiolase from Mycobacterium leprae (403 aa). Also highly similar to acyl/acetyl-CoA thiolases and beta-ketoadipyl CoA thiolases, e.g. T35428 probable acetyl CoA acetyltransferase (thiolase) from Streptomyces coelicolor (404 aa); NP_250427.1|NC_002516 probable acyl-CoA thiolase from Pseudomonas aeruginosa (401 aa); NP_106253.1|NC_002678 probable acyl-CoA thiolase from Mesorhizobium loti (402 aa); NP_248919.1|NC_002516|PcaF beta-ketoadipyl CoA thiolase PcaF from Pseudomonas aeruginosa (401 aa); etc. Contains PS00098 Thiolases acyl-enzyme intermediate signature, PS00737 Thiolases signature 2 and PS00099 Thiolases active site. O53871 No lipid metabolism ML2162 MMAR_4677 MSMEG_5721 Mb0882 NC_000962.3 Mycobrowser_v2 CDS 955077 956288 . + 0 Rv0859 fadA Function unknown, but involvement in lipid degradation. Possible acyl-CoA thiolase FadA Rv0859, (MTV043.52), len: 403 aa. Possible fadA, acyl-CoA thiolase, equivalent to NP_302423.1|NC_002677 putative beta-ketoadipyl CoA thiolase from Mycobacterium leprae (403 aa). Also highly similar to acyl/acetyl-CoA thiolases and beta-ketoadipyl CoA thiolases, e.g. T35428 probable acetyl CoA acetyltransferase (thiolase) from Streptomyces coelicolor (404 aa); NP_250427.1|NC_002516 probable acyl-CoA thiolase from Pseudomonas aeruginosa (401 aa); NP_106253.1|NC_002678 probable acyl-CoA thiolase from Mesorhizobium loti (402 aa); NP_248919.1|NC_002516|PcaF beta-ketoadipyl CoA thiolase PcaF from Pseudomonas aeruginosa (401 aa); etc. Contains PS00098 Thiolases acyl-enzyme intermediate signature, PS00737 Thiolases signature 2 and PS00099 Thiolases active site. O53871 No lipid metabolism ML2162 MMAR_4677 MSMEG_5721 Mb0882 NC_000962.3 Mycobrowser_v2 CDS 973806 974294 . - 0 Rv0875c Rv0875c Unknown Possible conserved exported protein Rv0875c, (MTCY31.03c), len: 162 aa. Possible conserved exported protein, equivalent to MLCB57_11|O33056 possible exported protein from Mycobacterium leprae (162 aa), FASTA scores: opt: 789, E(): 0, (71.4% identity in 161 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). P64731 No cell wall and cell processes ML2144 MMAR_4657 MSMEG_5694 Mb0899c NC_000962.3 Mycobrowser_v2 CDS 967344 967733 . - 0 Rv0870c Rv0870c Unknown Possible conserved integral membrane protein Rv0870c, (MTV043.63c), len: 129 aa. Possible conserved integral membrane protein, highly similar to other membrane proteins: putative secreted proteins or hypothetical proteins e.g. CAC08263.1| AL392146 putative integral membrane protein from Streptomyces coelicolor (138 aa); NP_233433.1|NC_002506 conserved hypothetical protein from Vibrio cholerae (143 aa); NP_455572.1|NC_003198 putative membrane protein from Salmonella enterica subsp. enterica serovar Typhi (148 aa); P37065|YCCF_ECOLI hypothetical 16.3 kDa protein from Escherichia coli (148 aa), FASTA scores: opt: 183, E(): 1.9e-06, (36.6% identity in 134 aa overlap); etc. O53882 No cell wall and cell processes ML2148 MMAR_4662 MSMEG_5697 Mb0894c NC_000962.3 Mycobrowser_v2 RBS 967738 967742 . - 0 Rv0870c Rv0870c Unknown Possible conserved integral membrane protein Rv0870c, (MTV043.63c), len: 129 aa. Possible conserved integral membrane protein, highly similar to other membrane proteins: putative secreted proteins or hypothetical proteins e.g. CAC08263.1| AL392146 putative integral membrane protein from Streptomyces coelicolor (138 aa); NP_233433.1|NC_002506 conserved hypothetical protein from Vibrio cholerae (143 aa); NP_455572.1|NC_003198 putative membrane protein from Salmonella enterica subsp. enterica serovar Typhi (148 aa); P37065|YCCF_ECOLI hypothetical 16.3 kDa protein from Escherichia coli (148 aa), FASTA scores: opt: 183, E(): 1.9e-06, (36.6% identity in 134 aa overlap); etc. O53882 No cell wall and cell processes ML2148 MMAR_4662 MSMEG_5697 Mb0894c NC_000962.3 Mycobrowser_v2 RBS 956279 956282 . + 0 Rv0860 fadB Involved in fatty acid degradation (probably in fatty acid beta-oxidation cycle). Probable fatty oxidation protein FadB Rv0860, (MTV043.53), len: 720 aa. Probable fadB, fatty oxidation protein, equivalent to NP_302422.1|NC_002677 putative fatty oxidation complex alpha subunit from Mycobacterium leprae (714 aa). Also highly similar to others and various proteins involved in fatty acid metabolism, e.g. T35429 probable fatty oxidation protein from Streptomyces coelicolor (733 aa); NP_250428.1|NC_002516 probable 3-hydroxyacyl-CoA dehydrogenase from Pseudomonas aeruginosa (714 aa); NP_418895.1|NC_002696 fatty oxidation complex alpha subunit from Caulobacter crescentus (709 aa); P40939|ECHA_HUMAN trifunctional enzyme alpha subunit [includes: long-chain enoyl-CoA hydratase ; long chain 3-hydroxyacyl-CoA dehydrogenase ] from Homo sapiens (763 aa), FASTA scores: opt: 1176, E(): 0, (32.4% identity in 722 aa overlap); P21177|FADB_ECOLI fatty oxidation complex alpha subunit [includes: enoyl-CoA hydratase; delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase; 3-hydroxyacyl-CoA dehydrogenase; 3- hydroxybutyryl-CoA epimerase] from Escherichia coli strain K12 (729 aa), FASTA scores: opt: 873, E(): 0, (33.6% identity in 693 aa overlap); etc. O53872 No lipid metabolism ML2161 MMAR_4676 MSMEG_5720 Mb0883 NC_000962.3 Mycobrowser_v2 CDS 956293 958455 . + 0 Rv0860 fadB Involved in fatty acid degradation (probably in fatty acid beta-oxidation cycle). Probable fatty oxidation protein FadB Rv0860, (MTV043.53), len: 720 aa. Probable fadB, fatty oxidation protein, equivalent to NP_302422.1|NC_002677 putative fatty oxidation complex alpha subunit from Mycobacterium leprae (714 aa). Also highly similar to others and various proteins involved in fatty acid metabolism, e.g. T35429 probable fatty oxidation protein from Streptomyces coelicolor (733 aa); NP_250428.1|NC_002516 probable 3-hydroxyacyl-CoA dehydrogenase from Pseudomonas aeruginosa (714 aa); NP_418895.1|NC_002696 fatty oxidation complex alpha subunit from Caulobacter crescentus (709 aa); P40939|ECHA_HUMAN trifunctional enzyme alpha subunit [includes: long-chain enoyl-CoA hydratase ; long chain 3-hydroxyacyl-CoA dehydrogenase ] from Homo sapiens (763 aa), FASTA scores: opt: 1176, E(): 0, (32.4% identity in 722 aa overlap); P21177|FADB_ECOLI fatty oxidation complex alpha subunit [includes: enoyl-CoA hydratase; delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase; 3-hydroxyacyl-CoA dehydrogenase; 3- hydroxybutyryl-CoA epimerase] from Escherichia coli strain K12 (729 aa), FASTA scores: opt: 873, E(): 0, (33.6% identity in 693 aa overlap); etc. O53872 No lipid metabolism ML2161 MMAR_4676 MSMEG_5720 Mb0883 NC_000962.3 Mycobrowser_v2 CDS 1138967 1142671 . + 0 Rv1020 mfd Involved in nucleotide excision repair. Necessary for strand-specific repair. A lesion in the template strand blocks the RNA polymerase complex (RNAP). The RNAP-DNA-RNA complex is specifically recognized by TRCF which releases RNAP and the truncated transcript; the TCRF may replace RNAP at the lesion site and then recruit the UVRA/B/C repair system. Probable transcription-repair coupling factor Mfd (TRCF) Rv1020, (MTCY10G2.29c), len: 1234 aa. Probable mfd (alternate gene name: trcF), transcription-repair coupling factor (see citation below), similar to many e.g. MFD_ECOLI|P30958 transcription-repair coupling factor from Escherichia coli (1148 aa), FASTA scores: opt: 1900, E(): 0, (37.9% identity in 1107 aa overlap); similar to M. tuberculosis Rv2973c and Rv1633. Contains PS00017 ATP/GTP-binding site motif A (P-loop). In the N-terminal section; belongs to the UVRB family. In the C-terminal section; belongs to the helicase family. RECG subfamily. P64326 No information pathways ML0252 MMAR_4465 MSMEG_5423 Mb1048 NC_000962.3 Mycobrowser_v2 tRNA 1025321 1025393 . - 0 MTB000014 argT - tRNA-Arg (CCT) argT, tRNA-Arg, anticodon cct, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 2340871 2341815 . + 0 Rv2083 Rv2083 Unknown Conserved hypothetical protein Rv2083, (MTCY49.22), len: 314 aa. Conserved hypothetical protein. Similar to many e.g. Mycobacterium tuberculosis Rv3898c (110 aa) and Rv3897c (210 aa). Q10691 No conserved hypotheticals Mb2109 NC_000962.3 Mycobrowser_v2 CDS 958523 960151 . - 0 Rv0861c ercc3 Involved in nucleotide excision repair. Has ATP-dependent 3' -> 5'helicase activity: acts by opening DNA either around the RNA transcription start site or the DNA damage. DNA helicase Ercc3 Rv0861c, (MTV043.54c), len: 542 aa. Ercc3, DNA helicase (see citation below), equivalent to NP_302420.1|NC_002677 probable DNA helicase from Mycobacterium leprae (549 aa). Also highly similar to others (shorter than several eukaryotic enzymes) e.g. NP_218820.1|NC_000919|AE001217|AE0 01217_6 putative DNA repair helicase from Treponema pallidum (606 aa), FASTA scores: opt: 1275, E(): 0, (47.5% identity in 592 aa overlap); Q00578|RA25_YEAST DNA repair helicase from Saccharomyces cerevisiae (843 aa), FASTA scores: opt: 777, E(): 0, (30.4% identity in 605 aa overlap); P49135|XPB_MOUSE DNA-repair protein complementing XP-B cells from Mus musculus (Mouse) (783 aa), FASTA scores: opt: 761, E(): 0, (36.3% identity in 375 aa overlap); etc. Seems to belong to the helicase family. Alternative nucleotide at position 958922 (C->a; A410A) has been observed. O53873 No information pathways ML2157 MMAR_4671 MSMEG_5706 Mb0884c NC_000962.3 Mycobrowser_v2 CDS 960342 962612 . - 0 Rv0862c Rv0862c Function unknown Conserved protein Rv0862c, (MTV043.55c), len: 756 aa. Conserved protein, equivalent to NP_302419.1|NC_002677 possible DNA-binding protein from Mycobacterium leprae (753 aa); and highly similar (except in C-terminus) to MLCB57.01|Z99494|T45333 hypothetical protein from Mycobacterium leprae (>577 aa, truncated), FASTA scores: opt: 3047, E(): 0, (78.9% identity in 578 aa overlap). Also similar in part to SCD12A.03c|AB93395.1|AL357524 hypothetical protein from Streptomyces coelicolor (867 aa). O53874 No conserved hypotheticals ML2156 MMAR_4670 MSMEG_5705 Mb0885c NC_000962.3 Mycobrowser_v2 CDS 962599 962880 . + 0 Rv0863 Rv0863 Function unknown Conserved hypothetical protein Rv0863, (MTV043.56), len: 93 aa. Conserved hypothetical protein, highly similar to NP_302418.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (74 aa). Also weakly similar in part to U82598|ECU82598_135 hypothetical protein from Escherichia coli, FASTA scores: (32.4% identity in 71 aa overlap); and M74011|YEPYSCOP_8 hypothetical protein from Yersinia enterocolitica (165 aa), FASTA scores: (38.6 identity in 57 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53875 No conserved hypotheticals ML2155 MMAR_4669 MSMEG_5704 Mb0886 NC_000962.3 Mycobrowser_v2 CDS 962890 963393 . + 0 Rv0864 moaC2 Involved in the biosynthesis of molybdopterin. Probable molybdenum cofactor biosynthesis protein C 2 MoaC2 Rv0864, (MTV043.57), len: 167 aa. Probable moaC2, molybdopterin cofactor biosynthesis protein, highly similar to others e.g. CAB59676.1|AL132674 molybdenum cofactor biosynthesis protein from Streptomyces coelicolor (170 aa); NP_418834.1|NC_002696 molybdenum cofactor biosynthesis protein C from Caulobacter crescentus (186 aa); Y10817|ANY10817_3|T44852 molybdopterin co-factor synthesis protein moaC from Arthrobacter nicotinovorans plasmid pAO1 (169 aa), FASTA scores: opt: 491, E(): 2.4e-29, (51.0% identity in 151 aa overlap); etc. Also highly similar to O05788|MOAC1|Rv3111|MTCY164.21 putative molybdenum cofactor biosynthesis protein C from Mycobacterium tuberculosis (170 aa), FASTA scores: opt: 491, E(): 2.4e-29, (54.9% identity in 153 aa overlap); and O53376|Rv3324c|MOAC3|MTV016.24c putative molybdenum cofactor biosynthesis protein C3 (177 aa). P0A5K6 No intermediary metabolism and respiration ML2154 MMAR_4668 MSMEG_5703 Mb0887,Mb0888 NC_000962.3 Mycobrowser_v2 RBS 3710253 3710258 . - 0 Rv0864 moaC2 Involved in the biosynthesis of molybdopterin. Probable molybdenum cofactor biosynthesis protein C 2 MoaC2 Rv0864, (MTV043.57), len: 167 aa. Probable moaC2, molybdopterin cofactor biosynthesis protein, highly similar to others e.g. CAB59676.1|AL132674 molybdenum cofactor biosynthesis protein from Streptomyces coelicolor (170 aa); NP_418834.1|NC_002696 molybdenum cofactor biosynthesis protein C from Caulobacter crescentus (186 aa); Y10817|ANY10817_3|T44852 molybdopterin co-factor synthesis protein moaC from Arthrobacter nicotinovorans plasmid pAO1 (169 aa), FASTA scores: opt: 491, E(): 2.4e-29, (51.0% identity in 151 aa overlap); etc. Also highly similar to O05788|MOAC1|Rv3111|MTCY164.21 putative molybdenum cofactor biosynthesis protein C from Mycobacterium tuberculosis (170 aa), FASTA scores: opt: 491, E(): 2.4e-29, (54.9% identity in 153 aa overlap); and O53376|Rv3324c|MOAC3|MTV016.24c putative molybdenum cofactor biosynthesis protein C3 (177 aa). P0A5K6 No intermediary metabolism and respiration ML2154 MMAR_4668 MSMEG_5703 Mb0887,Mb0888 NC_000962.3 Mycobrowser_v2 CDS 1025497 1026816 . - 0 Rv0920c Rv0920c Required for the transposition of the insertion element IS1554. Probable transposase Rv0920c, (MTCY21C12.14c), len: 439 aa. Probable transposase for IS1554, highly similar to others e.g. MTCY441.35|Q45111 transposase from Mycobacterium tuberculosis (419 aa), FASTA scores: opt: 1113, E(): 0, (43.9% identity in 378 aa overlap); etc. Contains transposases mutator family signature (PS01007). O05912 No insertion seqs and phages Mb0944c NC_000962.3 Mycobrowser_v2 CDS 974291 975937 . - 0 Rv0876c Rv0876c Unknown Possible conserved transmembrane protein Rv0876c, (MTCY31.04c), len: 548 aa. Possible conserved transmembrane protein, equivalent to MLCB57_12|O33057 possible membrane protein from Mycobacterium leprae (579 aa), FASTA scores: opt: 2850, E(): 0, (81.0% identity in 568 aa overlap). Also highly similar (except in N-terminus) to CAB93403.1|AL357524 putative integral membrane protein from Streptomyces coelicolor (463 aa). Q10564 No cell wall and cell processes ML2143 MMAR_4656 MSMEG_5693 Mb0900c NC_000962.3 Mycobrowser_v2 RBS 975949 975957 . - 0 Rv0876c Rv0876c Unknown Possible conserved transmembrane protein Rv0876c, (MTCY31.04c), len: 548 aa. Possible conserved transmembrane protein, equivalent to MLCB57_12|O33057 possible membrane protein from Mycobacterium leprae (579 aa), FASTA scores: opt: 2850, E(): 0, (81.0% identity in 568 aa overlap). Also highly similar (except in N-terminus) to CAB93403.1|AL357524 putative integral membrane protein from Streptomyces coelicolor (463 aa). Q10564 No cell wall and cell processes ML2143 MMAR_4656 MSMEG_5693 Mb0900c NC_000962.3 Mycobrowser_v2 CDS 967898 968305 . + 0 Rv0871 cspB Function unknown; thought to act in response to low temperature. Probable cold shock-like protein B CspB Rv0871, (MTV043.64), len: 135 aa. Probable cspB, cold shock-like protein B, equivalent to Z99494|MLCB57_7|MLCB57.11 probable cold shock protein from Mycobacterium leprae (136 aa), FASTA scores: opt: 787, E(): 0, (86.0% identity in 136 aa overlap). Also highly similar (but often longer than) to others e.g. CAB93399.1|AL357524 cold shock protein B from Streptomyces coelicolor (127 aa); Q45099|CSPD_BACCE cold shock-like protein CSPD from Bacillus cereus (66 aa); Y101 81|LLCSPB_1 cold shock protein from Lactococcus lactis (66 aa), FASTA scores: opt: 220, E(): 2.5e-07, (48.3% identity in 60 aa overlap); etc. Seems to belong to the cold-shock domain (CSD) family. O53883 No virulence, detoxification, adaptation ML2147 MMAR_4661 MSMEG_5696 Mb0895 NC_000962.3 Mycobrowser_v2 CDS 963390 963872 . + 0 Rv0865 mog Involved in molybdopterin biosynthesis; involved in the biosynthesis of a demolybdo-cofactor (molybdopterin), necessary for molybdo-enzymes. Probable molybdopterin biosynthesis Mog protein Rv0865, (MTV043.58), len: 160 aa. Probable mog, molybdopterin biosynthesis MOG protein, highly similar or similar to other molybdenum cofactor biosynthesis proteins e.g. CAB59675.1|AL132674 molybdenum cofactor biosynthesis protein from Streptomyces coelicolor (179 aa); NP_301253.1|NC_002677 putative molybdenum cofactor biosynthesis protein from Mycobacterium leprae (181 aa); CAC39235.1|AJ312124 Mog protein from Eubacterium acidaminophilum (162 aa); P44645|MOG_HAEIN|MOGA|HI0336 molybdopterin biosynthesis MOG protein from Haemophilus influenzae (197 aa), FASTA scores: opt: 306, E(): 9e-13, (39.6% identity in 139 aa overlap); P28694|MOG_ECOLI molybdopterin biosynthesis MOG protein from Escherichia coli (195 aa), FASTA scores: opt: 265, E(): 3.6e-10, (34.2 identity in 146 aa overlap); etc. Also highly similar to Rv0984|MTV044.12|MOAB2 possible pterin-4-alpha-carbinolamine dehydratase from Mycobacterium tuberculosis (181 aa). O53877 No intermediary metabolism and respiration ML2153 MMAR_4667 MSMEG_5702 Mb0889 NC_000962.3 Mycobrowser_v2 CDS 963869 964294 . + 0 Rv0866 moaE2 Possibly a molybdenum biosynthesis cofactor. Conversion of molybdopterin precursor Z into molybdopterin requires transfer of two sulfur atoms to precursor Z (to generate the dithiolene group). This is catalyzed by the converting factor composed of a small and large subunit. Probable molybdenum cofactor biosynthesis protein E2 MoaE2 (molybdopterin converting factor large subunit) (molybdopterin [MPT] converting factor, subunit 2) Rv0866, (MTV043.59), len: 141 aa. Probable moaE2, molybdopterin converting factor E (molybdopterin converting factor (subunit 2)), similar to others e.g. Y10817|ANY10817_4|T44853 molybdopterin biosynthesis protein E chain from Arthrobacter nicotinovorans plasmid pAO1 (155 aa), FASTA scores: opt: 460, E(): 3.5e-27, (49.3 identity in 146 aa overlap); CAC01331.1|AL390968 moaE-like protein from Streptomyces coelicolor (152 aa); NP_389313.1|NC_000964 molybdopterin converting factor (subunit 2) from Bacillus subtilis (157 aa); etc. Also highly similar to Rv3119|MOAE1|Z95150|MTCY164_30 putative molybdenum cofactor biosynthesis protein E from Mycobacterium tuberculosis (147 aa), FASTA scores: opt: 321, E(): 5.9e-17, (40.9% identity in 132 aa overlap); and O53375|GPHA|Rv3323c|MTV016.23c MOAD-MOAE fusion protein from Mycobacterium tuberculosis (221 aa). O53878 No intermediary metabolism and respiration ML2152 MMAR_4666 MSMEG_5701 Mb0890 NC_000962.3 Mycobrowser_v2 RBS 3485121 3485124 . + 0 Rv0866 moaE2 Possibly a molybdenum biosynthesis cofactor. Conversion of molybdopterin precursor Z into molybdopterin requires transfer of two sulfur atoms to precursor Z (to generate the dithiolene group). This is catalyzed by the converting factor composed of a small and large subunit. Probable molybdenum cofactor biosynthesis protein E2 MoaE2 (molybdopterin converting factor large subunit) (molybdopterin [MPT] converting factor, subunit 2) Rv0866, (MTV043.59), len: 141 aa. Probable moaE2, molybdopterin converting factor E (molybdopterin converting factor (subunit 2)), similar to others e.g. Y10817|ANY10817_4|T44853 molybdopterin biosynthesis protein E chain from Arthrobacter nicotinovorans plasmid pAO1 (155 aa), FASTA scores: opt: 460, E(): 3.5e-27, (49.3 identity in 146 aa overlap); CAC01331.1|AL390968 moaE-like protein from Streptomyces coelicolor (152 aa); NP_389313.1|NC_000964 molybdopterin converting factor (subunit 2) from Bacillus subtilis (157 aa); etc. Also highly similar to Rv3119|MOAE1|Z95150|MTCY164_30 putative molybdenum cofactor biosynthesis protein E from Mycobacterium tuberculosis (147 aa), FASTA scores: opt: 321, E(): 5.9e-17, (40.9% identity in 132 aa overlap); and O53375|GPHA|Rv3323c|MTV016.23c MOAD-MOAE fusion protein from Mycobacterium tuberculosis (221 aa). O53878 No intermediary metabolism and respiration ML2152 MMAR_4666 MSMEG_5701 Mb0890 NC_000962.3 Mycobrowser_v2 CDS 964312 965535 . - 0 Rv0867c rpfA Unknown. May be promote the resuscitation and growth of dormant, nongrowing cell. Possible resuscitation-promoting factor RpfA Rv0867c, (MTV043.60c), len: 407 aa. Possible rpfA, resuscitation-promoting factor (see citation below). N-terminus highly similar to N-terminal part (1-125 aa) of Z99494|MLCB57_3|NP_302417.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (174 aa), FASTA scores: opt: 785, E(): 1.8e-18, (63.0% identity in 200 aa overlap); and highly similar to C-terminus of NP_301299.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (375 aa); and middle part of NP_302360.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (157 aa). N-terminus also highly similar in part of three secreted proteins from Streptomyces coelicolor e.g. CAC09538.1|AL442120 putative secreted protein (244 aa). Regions highly similar to CAB76321.1|AL158060 putative membrane protein from Streptomyces coelicolor (121 aa); and middle part of CAB09664.1|Z96935 rpf from Micrococcus luteus (220 aa). Also highly similar in part to four resuscitation-promoting factors from Mycobacterium tuberculosis: Rv2450 (172 aa), Rv1009 (362 aa), Rv1884c (176 aa), and Rv2389c (154 aa). Contains a probable secretory signal sequence in N-terminus. Predicted possible vaccine candidate (See Zvi et al., 2008). O53879 No cell wall and cell processes ML2151 MMAR_4665 MSMEG_5700 Mb0891c NC_000962.3 Mycobrowser_v2 RBS 965547 965551 . - 0 Rv0867c rpfA Unknown. May be promote the resuscitation and growth of dormant, nongrowing cell. Possible resuscitation-promoting factor RpfA Rv0867c, (MTV043.60c), len: 407 aa. Possible rpfA, resuscitation-promoting factor (see citation below). N-terminus highly similar to N-terminal part (1-125 aa) of Z99494|MLCB57_3|NP_302417.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (174 aa), FASTA scores: opt: 785, E(): 1.8e-18, (63.0% identity in 200 aa overlap); and highly similar to C-terminus of NP_301299.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (375 aa); and middle part of NP_302360.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (157 aa). N-terminus also highly similar in part of three secreted proteins from Streptomyces coelicolor e.g. CAC09538.1|AL442120 putative secreted protein (244 aa). Regions highly similar to CAB76321.1|AL158060 putative membrane protein from Streptomyces coelicolor (121 aa); and middle part of CAB09664.1|Z96935 rpf from Micrococcus luteus (220 aa). Also highly similar in part to four resuscitation-promoting factors from Mycobacterium tuberculosis: Rv2450 (172 aa), Rv1009 (362 aa), Rv1884c (176 aa), and Rv2389c (154 aa). Contains a probable secretory signal sequence in N-terminus. Predicted possible vaccine candidate (See Zvi et al., 2008). O53879 No cell wall and cell processes ML2151 MMAR_4665 MSMEG_5700 Mb0891c NC_000962.3 Mycobrowser_v2 CDS 1418579 1418953 . - 0 Rv1269c Rv1269c Unknown Conserved probable secreted protein Rv1269c, (MTCY50.13), len: 124 aa. Conserved probable exported protein with putative N-terminal signal sequence. Similar to Mycobacterium tuberculosis protein Rv1813c|Y0DU_MYCTU|Q50620 hypothetical protein cy1a11.30 (137 aa), FASTA scores: E(): 9e-21, (41.6% identity in 137 aa overlap). P0A5E1 No cell wall and cell processes MMAR_4153 Mb1300c NC_000962.3 Mycobrowser_v2 RBS 1418961 1418967 . - 0 Rv1269c Rv1269c Unknown Conserved probable secreted protein Rv1269c, (MTCY50.13), len: 124 aa. Conserved probable exported protein with putative N-terminal signal sequence. Similar to Mycobacterium tuberculosis protein Rv1813c|Y0DU_MYCTU|Q50620 hypothetical protein cy1a11.30 (137 aa), FASTA scores: E(): 9e-21, (41.6% identity in 137 aa overlap). P0A5E1 No cell wall and cell processes MMAR_4153 Mb1300c NC_000962.3 Mycobrowser_v2 CDS 965983 966261 . - 0 Rv0868c moaD2 Involved in molybdenum cofactor biosynthesis. Probable molybdenum cofactor biosynthesis protein D 2 MoaD2 (molybdopterin converting factor small subunit) (molybdopterin [MPT] converting factor, subunit 1) Rv0868c, (MTV043.61c), len: 92 aa. Probable moaD2, molybdenum cofactor biosynthesis protein (molybdopterin converting factor (subunit 1)), similar to CAB88494.1|AL353816 putative molybdopterin converting factor from Streptomyces coelicolor (84 aa); and weakly similar to others MoaD proteins e.g. Z99111|BSUB0008_103 from Bacillus subtilis (77 aa), FASTA scores: opt: 86, E(): 2.8, (22.9% identity in 83 aa overlap); etc. Also some similarity with Rv3112|MOAD1|MTCY164.22 putative molybdenum cofactor biosynthesis protein D from Mycobacterium tuberculosis (83 aa), FASTA scores: opt: 113, E(): 0.024, (31.3% identity in 83 aa overlap). O53880 No intermediary metabolism and respiration ML2150 MMAR_4664 MSMEG_5699 Mb0892c NC_000962.3 Mycobrowser_v2 RBS 966269 966273 . - 0 Rv0868c moaD2 Involved in molybdenum cofactor biosynthesis. Probable molybdenum cofactor biosynthesis protein D 2 MoaD2 (molybdopterin converting factor small subunit) (molybdopterin [MPT] converting factor, subunit 1) Rv0868c, (MTV043.61c), len: 92 aa. Probable moaD2, molybdenum cofactor biosynthesis protein (molybdopterin converting factor (subunit 1)), similar to CAB88494.1|AL353816 putative molybdopterin converting factor from Streptomyces coelicolor (84 aa); and weakly similar to others MoaD proteins e.g. Z99111|BSUB0008_103 from Bacillus subtilis (77 aa), FASTA scores: opt: 86, E(): 2.8, (22.9% identity in 83 aa overlap); etc. Also some similarity with Rv3112|MOAD1|MTCY164.22 putative molybdenum cofactor biosynthesis protein D from Mycobacterium tuberculosis (83 aa), FASTA scores: opt: 113, E(): 0.024, (31.3% identity in 83 aa overlap). O53880 No intermediary metabolism and respiration ML2150 MMAR_4664 MSMEG_5699 Mb0892c NC_000962.3 Mycobrowser_v2 RBS 3709725 3709729 . - 0 Rv0868c moaD2 Involved in molybdenum cofactor biosynthesis. Probable molybdenum cofactor biosynthesis protein D 2 MoaD2 (molybdopterin converting factor small subunit) (molybdopterin [MPT] converting factor, subunit 1) Rv0868c, (MTV043.61c), len: 92 aa. Probable moaD2, molybdenum cofactor biosynthesis protein (molybdopterin converting factor (subunit 1)), similar to CAB88494.1|AL353816 putative molybdopterin converting factor from Streptomyces coelicolor (84 aa); and weakly similar to others MoaD proteins e.g. Z99111|BSUB0008_103 from Bacillus subtilis (77 aa), FASTA scores: opt: 86, E(): 2.8, (22.9% identity in 83 aa overlap); etc. Also some similarity with Rv3112|MOAD1|MTCY164.22 putative molybdenum cofactor biosynthesis protein D from Mycobacterium tuberculosis (83 aa), FASTA scores: opt: 113, E(): 0.024, (31.3% identity in 83 aa overlap). O53880 No intermediary metabolism and respiration ML2150 MMAR_4664 MSMEG_5699 Mb0892c NC_000962.3 Mycobrowser_v2 CDS 966265 967347 . - 0 Rv0869c moaA2 Involved in molybdenum cofactor biosynthesis; involved in the biosynthesis of molybdopterin precursor Z from guanosine. Probable molybdenum cofactor biosynthesis protein A2 MoaA2 Rv0869c, (MTV043.62c), len: 360 aa. Probable moaA2, molybdenum cofactor biosynthesis protein, highly similar to others e.g. CAB59437.1|AL132644|SCI8_6 molybdenum cofactor biosynthesis protein A from Streptomyces coelicolor (341 aa), FASTA scores: opt: 1336, E(): 0, (61.7% identity in 332 aa overlap); S57490|X78980|ANMOAA_1 molybdopterin cofactor synthesis protein from Arthrobacter nicotinovorans (fragment) (374 aa), FASTA scores: opt: 1059, E(): 0, (49.9% identity in 369 aa overlap); Q44118|MOAA_ARTNI probable molybdopterin cofactor synthesis protein A from Arthrobacter nicotinovorans plasmid pAO1 (355 aa); etc. Also similar to Rv3109|MTCY164.19|Z95150|MOAA1 putative molybdenum cofactor biosynthesis protein A from Mycobacterium tuberculosis (359 aa), FASTA scores: opt: 657, E(): 0, (36.6% identity in 309 aa overlap). Belongs to the MoaA / NifB / PqqE family. P65384 No intermediary metabolism and respiration ML2149 MMAR_4663 MSMEG_5698 Mb0893c NC_000962.3 Mycobrowser_v2 CDS 968424 970244 . - 0 Rv0872c PE_PGRS15 Function unknown PE-PGRS family protein PE_PGRS15 Rv0872c, (MTV043.65c), len: 606 aa. PE_PGRS15, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002), similar to many e.g. MTCY24A1.04c|Z95207 (615 aa), FASTA scores: opt: 2636, E(): 0, (64.6% identity in 619 aa overlap); etc. Q79FV3 No PE/PPE Mb0896c NC_000962.3 Mycobrowser_v2 RBS 970251 970255 . - 0 Rv0872c PE_PGRS15 Function unknown PE-PGRS family protein PE_PGRS15 Rv0872c, (MTV043.65c), len: 606 aa. PE_PGRS15, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002), similar to many e.g. MTCY24A1.04c|Z95207 (615 aa), FASTA scores: opt: 2636, E(): 0, (64.6% identity in 619 aa overlap); etc. Q79FV3 No PE/PPE Mb0896c NC_000962.3 Mycobrowser_v2 CDS 3127364 3128272 . - 0 Rv2820c Rv2820c Unknown Hypothetical protein Rv2820c, (MTCY16B7.22), len: 302 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P71632 No conserved hypotheticals Mb2844c NC_000962.3 Mycobrowser_v2 RBS 970495 970499 . + 0 Rv0873 fadE10 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE10 Rv0873, (MTV043.66-MTCY31.01), len: 650 aa. Probable fadE10, acyl-CoA dehydrogenase, highly similar to many e.g. CAB91129.1|AL355913 putative acyl CoA dehydrogenase from Streptomyces coelicolor (658 aa); P50544|ACDV_MOUSE acyl-CoA dehydrogenase from Mus musculus (656 aa); D30647|RATVLCAD_1 very-long-chain Acyl-CoA dehydrogenase from Rattus norvegicus (655 aa), FASTA scores: opt: 675, E(): 0, (33.9% identity in 380 aa overlap); etc. P63429 No lipid metabolism ML2146 MMAR_4660 Mb0897 NC_000962.3 Mycobrowser_v2 CDS 970505 972457 . + 0 Rv0873 fadE10 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE10 Rv0873, (MTV043.66-MTCY31.01), len: 650 aa. Probable fadE10, acyl-CoA dehydrogenase, highly similar to many e.g. CAB91129.1|AL355913 putative acyl CoA dehydrogenase from Streptomyces coelicolor (658 aa); P50544|ACDV_MOUSE acyl-CoA dehydrogenase from Mus musculus (656 aa); D30647|RATVLCAD_1 very-long-chain Acyl-CoA dehydrogenase from Rattus norvegicus (655 aa), FASTA scores: opt: 675, E(): 0, (33.9% identity in 380 aa overlap); etc. P63429 No lipid metabolism ML2146 MMAR_4660 Mb0897 NC_000962.3 Mycobrowser_v2 RBS 976064 976067 . + 0 Rv0877 Rv0877 Function unknown Conserved hypothetical protein Rv0877, (MTCY31.05), len: 262 aa. Conserved hypothetical protein, equivalent to MLCB57_13|O33058 conserved hypothetical protein from Mycobacterium leprae (269 aa), FASTA scores: E(): 0, (80.5% identity in 257 aa overlap). Also highly similar (except in C-terminus) to SCD12A.13|CAB93404.1|AL357524 hypothetical protein from Streptomyces coelicolor (308 aa). P64733 No conserved hypotheticals ML2142 MMAR_4655 MSMEG_5691 Mb0901 NC_000962.3 Mycobrowser_v2 CDS 976075 976863 . + 0 Rv0877 Rv0877 Function unknown Conserved hypothetical protein Rv0877, (MTCY31.05), len: 262 aa. Conserved hypothetical protein, equivalent to MLCB57_13|O33058 conserved hypothetical protein from Mycobacterium leprae (269 aa), FASTA scores: E(): 0, (80.5% identity in 257 aa overlap). Also highly similar (except in C-terminus) to SCD12A.13|CAB93404.1|AL357524 hypothetical protein from Streptomyces coelicolor (308 aa). P64733 No conserved hypotheticals ML2142 MMAR_4655 MSMEG_5691 Mb0901 NC_000962.3 Mycobrowser_v2 CDS 976872 978203 . - 0 Rv0878c PPE13 Function unknown PPE family protein PPE13 Rv0878c, (MTCY31.06c), len: 443 aa. PPE13, Member of the Mycobacterium tuberculosis PPE family, highly similar to many e.g. P4261|YHS6_MYCTU (517 aa), FASTA scores: opt: 1044, E(): 0, (47.4% identity in 397 aa overlap); MTV014_3, MTCI65_2, MTCY98_24, MTCY3C7_23, MTCY48_17, MTV004_5, MTV004_3, etc. Predicted to be an outer membrane protein (See Song et al., 2008). Q10540 No PE/PPE MMAR_4319 Mb0902c NC_000962.3 Mycobrowser_v2 RBS 978211 978213 . - 0 Rv0878c PPE13 Function unknown PPE family protein PPE13 Rv0878c, (MTCY31.06c), len: 443 aa. PPE13, Member of the Mycobacterium tuberculosis PPE family, highly similar to many e.g. P4261|YHS6_MYCTU (517 aa), FASTA scores: opt: 1044, E(): 0, (47.4% identity in 397 aa overlap); MTV014_3, MTCI65_2, MTCY98_24, MTCY3C7_23, MTCY48_17, MTV004_5, MTV004_3, etc. Predicted to be an outer membrane protein (See Song et al., 2008). Q10540 No PE/PPE MMAR_4319 Mb0902c NC_000962.3 Mycobrowser_v2 CDS 978481 978756 . - 0 Rv0879c Rv0879c Unknown Possible conserved transmembrane protein Rv0879c, (MTCY31.07c), len: 91 aa. Possible conserved transmembrane protein, C-terminus highly similar to C-terminal part of MLCB57_14|O33059 conserved hypothetical protein from Mycobacterium leprae (91 aa), FASTA scores: E(): 1.2e-25, (76.9% identity in 91 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P64735 No cell wall and cell processes ML2141 MMAR_4653 MSMEG_5689 Mb0903c NC_000962.3 Mycobrowser_v2 CDS 978934 979365 . + 0 Rv0880 Rv0880 Thought to be involved in transcriptional mechanism. Possible transcriptional regulatory protein (possibly MarR-family) Rv0880, (MTCY31.08), len: 143 aa. Possible transcriptional regulator, MarR family, equivalent to MLCB57_15|O3306|NP_302411.1|NC_002677 putative MarR-family protein from Mycobacterium leprae (143 aa), FASTA scores: opt: 818, E(): 0, (89.5% identity in 143 aa overlap). Also similar to many others e.g. CAB93410.1|AL357524 putative marR-family protein from Streptomyces coelicolor (145 aa); NP_251757.1|NC_002516 probable transcriptional regulator from Pseudomonas aeruginosa (147 aa); etc. Also similar to Rv2327 from Mycobacterium tuberculosis (163 aa). P67745 No regulatory proteins ML2140 Mb0904 NC_000962.3 Mycobrowser_v2 CDS 979362 980228 . + 0 Rv0881 Rv0881 Causes methylation Possible rRNA methyltransferase (rRNA methylase) Rv0881, (MTCY31.09), len: 288 aa. Possible rRNA methyltransferase, highly similar to others and hypothetical proteins e.g. CAB76071.1|AL157953 putative rRNA methylase from Streptomyces coelicolor (272 aa); NP_421117.1|NC_002696 spoU rRNA methylase family protein from Caulobacter crescentus (268 aa); D90913_93|P74261 rRNA methylase from Synechocystis sp. (274 aa), FASTA scores: E(): 1.1e-13, (26.3% identity in 278 aa overlap); P18644|TSNR_STRCN rRNA methyltransferase from Streptomyces cyaneus (Streptomyces curacoi) (269 aa), FASTA scores: E(): 3.7e-08, (23.9% identity in 268 aa overlap); etc. Equivalent to AAK45146.1 from Mycobacterium tuberculosis strain CDC1551 (242 aa) but longer 46 aa. Q10543 No intermediary metabolism and respiration ML2139 MMAR_4651 MSMEG_5687 Mb0905 NC_000962.3 Mycobrowser_v2 CDS 980225 980509 . + 0 Rv0882 Rv0882 Unknown Probable transmembrane protein Rv0882, (MTCY31.10), len: 94 aa. Probable transmembrane protein. P64737 No cell wall and cell processes ML2138 MMAR_4650 MSMEG_5686 Mb0906 NC_000962.3 Mycobrowser_v2 CDS 980506 981267 . - 0 Rv0883c Rv0883c Function unknown Conserved hypothetical protein Rv0883c, (MTCY31.11c), len: 253 aa. Conserved hypothetical protein, equivalent to O3306|MLCB57_16 conserved hypothetical protein from Mycobacterium leprae (251 aa), FASTA scores: E(): 0, (79.4% identity in 253 aa overlap). Also highly similar to N_terminus of AL009204|SC9B10_22 hypothetical protein from Streptomyces coelicolor (352 aa), FASTA scores: E(): 6.1e-20, (35.0% identity in 246 aa overlap). P64739 No conserved hypotheticals ML2137 MMAR_4649 MSMEG_5685 Mb0907c NC_000962.3 Mycobrowser_v2 RBS 981273 981277 . - 0 Rv0883c Rv0883c Function unknown Conserved hypothetical protein Rv0883c, (MTCY31.11c), len: 253 aa. Conserved hypothetical protein, equivalent to O3306|MLCB57_16 conserved hypothetical protein from Mycobacterium leprae (251 aa), FASTA scores: E(): 0, (79.4% identity in 253 aa overlap). Also highly similar to N_terminus of AL009204|SC9B10_22 hypothetical protein from Streptomyces coelicolor (352 aa), FASTA scores: E(): 6.1e-20, (35.0% identity in 246 aa overlap). P64739 No conserved hypotheticals ML2137 MMAR_4649 MSMEG_5685 Mb0907c NC_000962.3 Mycobrowser_v2 CDS 981424 982554 . - 0 Rv0884c serC Catalyzes the reversible interconversion of phosphoserine and 2-oxoglutarate to 3-phosphonooxypyruvate and glutamate. Require both in the major phosphorylated pathway of serine biosynthesis and in pyridoxine biosynthesis [catalytic activity: O-phospho-L-serine + 2-oxoglutarate = 3-phosphonooxypyruvate + L-glutamate]. Possible phosphoserine aminotransferase SerC (PSAT) Rv0884c, (MTCY31.12c), len: 376 aa. Possible serC, phosphoserine aminotransferase, equivalent to MLCB57_17 putative phosphoserine aminotransferase from Mycobacterium leprae (376 aa), FASTA scores: E(): 0, (87.5 identity in 376 aa overlap). Also highly similar to CAC08322.1|AL392149 putative aminotransferase from Streptomyces coelicolor (363 aa); and similar to other phosphoserine aminotransferases e.g. NP_386837.1|NC_003047 putative phosphoserine aminotransferase protein from Sinorhizobium meliloti (392 aa); P52878|SERC_METBA phosphoserine aminotransferase from Methanosarcina barkeri (370 aa); P10658|SERC_RABIT|RABEPIP_1 phosphoserine aminotransferase from Rabbit (370 aa), FASTA scores: opt: 271, E(): 3.5e-11, (24.5% identity in 368 aa overlap); etc. Belongs to class-V of pyridoxal-phosphate-dependent aminotransferases. Cofactor: pyridoxal phosphate. P63514 No intermediary metabolism and respiration ML2136 MMAR_4648 MSMEG_5684 Mb0908c NC_000962.3 Mycobrowser_v2 RBS 982751 982755 . + 0 Rv0885 Rv0885 Function unknown Conserved hypothetical protein Rv0885, (MTCY31.13), len: 340 aa. Conserved hypothetical protein, equivalent to O33063|MLCB57_18 possible transmembrane protein from Mycobacterium leprae (341 aa), FASTA score: (83.9% identity in 341 aa overlap). Also similar except in C-terminus to T35630 probable membrane protein from Streptomyces coelicolor (312 aa). P0A5D5 No conserved hypotheticals ML2135 MMAR_4647 MSMEG_5682 Mb0909 NC_000962.3 Mycobrowser_v2 CDS 982762 983784 . + 0 Rv0885 Rv0885 Function unknown Conserved hypothetical protein Rv0885, (MTCY31.13), len: 340 aa. Conserved hypothetical protein, equivalent to O33063|MLCB57_18 possible transmembrane protein from Mycobacterium leprae (341 aa), FASTA score: (83.9% identity in 341 aa overlap). Also similar except in C-terminus to T35630 probable membrane protein from Streptomyces coelicolor (312 aa). P0A5D5 No conserved hypotheticals ML2135 MMAR_4647 MSMEG_5682 Mb0909 NC_000962.3 Mycobrowser_v2 CDS 983803 985530 . + 0 Rv0886 fprB Serves as the first electron transfer protein in all the P450 systems [catalytic activity: reduced adrenodoxin + NADP+ = oxidized adrenodoxin + NADPH]. Probable NADPH:adrenodoxin oxidoreductase FprB (adrenodoxin reductase) (AR) (ferredoxin-NADP(+) reductase) Rv0886, (MTCY31.14), len: 575 aa. Probable fprB, ferredoxin/ferredoxin-NADP(+) reductase (NADPH:adrenodoxin oxidoreductase), equivalent to O3306|MLCB57_19 ferredoxin/ferredoxin--NADP reductase from Mycobacterium leprae (555 aa), FASTA scores: E(): 0, (76.6 identity in 560 aa overlap). Also highly similar or similar to others e.g. NP_294219.1|NC_001263 putative ferredoxin/ferredoxin--NADP reductase from Deinococcus radiodurans (479 aa) (N-terminus shorter); P22570|ADRO_HUMAN NADPH:adrenodoxin oxidoreductase from homo sapiens (497 aa), FASTA scores: opt: 624, E(): 3e-30, (39.7% identity in 484 aa overlap); P08165|ADRO_BOVIN NADPH:adrenodoxin oxidoreductase from Bos taurus (492 aa); etc. Also similar to others from Mycobacterium tuberculosis e.g. Rv3106, Rv3858c, etc. Contains PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature. P65528 No intermediary metabolism and respiration ML2134 MMAR_4646 MSMEG_5681 Mb0910 NC_000962.3 Mycobrowser_v2 RBS 987222 987227 . + 0 Rv0888 Rv0888 Unknown Probable exported protein Rv0888, (MTCY31.16), len: 490 aa. Probable exported protein. Equivalent to AAK45157.1 from Mycobacterium tuberculosis strain CDC1551 (507 aa) but shorter 17 aa. Contains possible N-terminal signal sequence. Predicted to be an outer membrane protein (See Song et al., 2008). P64743 No cell wall and cell processes MMAR_4644 Mb0912 NC_000962.3 Mycobrowser_v2 CDS 987233 988705 . + 0 Rv0888 Rv0888 Unknown Probable exported protein Rv0888, (MTCY31.16), len: 490 aa. Probable exported protein. Equivalent to AAK45157.1 from Mycobacterium tuberculosis strain CDC1551 (507 aa) but shorter 17 aa. Contains possible N-terminal signal sequence. Predicted to be an outer membrane protein (See Song et al., 2008). P64743 No cell wall and cell processes MMAR_4644 Mb0912 NC_000962.3 Mycobrowser_v2 CDS 1426164 1427417 . + 0 Rv1277 Rv1277 Function unknown Conserved hypothetical protein Rv1277, (MTCY50.05c), len: 417 aa. Conserved hypothetical protein, some similarity to 3914967|O68033|SBCD_RHOCA exonuclease SBCD homolog from Rhodobacter capsulatus (405 aa). May be sbcD protein (see Mizrahi & Andersen 1998) Q50699 No conserved hypotheticals ML1119 MMAR_4143 MSMEG_5004 Mb1308 NC_000962.3 Mycobrowser_v2 CDS 988740 989861 . - 0 Rv0889c citA Involved in tricarboxylic acid cycle (KREBS cycle) [catalytic activity: citrate + CoA = acetyl-CoA + H2O + oxaloacetate]. Probable citrate synthase II CitA Rv0889c, (MTCY31.17c), len: 373 aa. Probable citA (alternate gene name: gltA), citrate synthase 2, highly similar to others e.g. CAB95899.1|AL359988 putative citrate synthase from Streptomyces coelicolor (387 aa); P39119|CISY_BACSU citrate synthase II from Bacillus subtilis (366 aa), FASTA scores: opt: 586, E(): 5.8e-30, (33.8% identity in 367 aa overlap); etc. Also similar to Rv0896|MTCY31.24 from Mycobacterium tuberculosis (29.2% identity in 274 aa overlap) and Rv1131. Contains PS00480 Citrate synthase signature. Belongs to the citrate synthase family. P63777 No intermediary metabolism and respiration ML2132 MMAR_4643 MSMEG_5676 Mb0913c NC_000962.3 Mycobrowser_v2 RBS 989875 989879 . - 0 Rv0889c citA Involved in tricarboxylic acid cycle (KREBS cycle) [catalytic activity: citrate + CoA = acetyl-CoA + H2O + oxaloacetate]. Probable citrate synthase II CitA Rv0889c, (MTCY31.17c), len: 373 aa. Probable citA (alternate gene name: gltA), citrate synthase 2, highly similar to others e.g. CAB95899.1|AL359988 putative citrate synthase from Streptomyces coelicolor (387 aa); P39119|CISY_BACSU citrate synthase II from Bacillus subtilis (366 aa), FASTA scores: opt: 586, E(): 5.8e-30, (33.8% identity in 367 aa overlap); etc. Also similar to Rv0896|MTCY31.24 from Mycobacterium tuberculosis (29.2% identity in 274 aa overlap) and Rv1131. Contains PS00480 Citrate synthase signature. Belongs to the citrate synthase family. P63777 No intermediary metabolism and respiration ML2132 MMAR_4643 MSMEG_5676 Mb0913c NC_000962.3 Mycobrowser_v2 CDS 989948 992596 . - 0 Rv0890c Rv0890c Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably LuxR-family) Rv0890c, (MTCY31.18c), len: 882 aa. Probable transcriptional regulatory protein, LuxR family, highly similar (but shorter 238 aa in N-terminus) to NP_302202.1|NC_002677 possible transcriptional regulator from Mycobacterium leprae (1106 aa). Also highly similar (generally in part) to others e.g. T50568 probable multi-domain regulatory protein from Streptomyces coelicolor (1334 aa); P10957|NARL_ECOLI nitrate/nitrite response regulator protein from Escherichia coli (216 aa), FASTA scores: opt: 193, E(): 6e-06, (37.4% identity in 99 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. MTCY02B10_22, MTV008_44, MTV036_21, and MTCY31_24. Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00622 Bacterial regulatory proteins, luxR family signature, and probable helix-turn helix motif from aa 836 to 857 (Score 1559, +4.50 SD). Belongs to the LuxR/UhpA family of transcriptional regulators. Alternative nucleotide at position 990001 (G->C; P866A) has been observed. Q10550 No regulatory proteins Mb0914c NC_000962.3 Mycobrowser_v2 CDS 992598 993455 . - 0 Rv0891c Rv0891c Thought to be involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0891c, (MTCY31.19c), len: 285 aa. Possible transcriptional regulator, highly similar in N-terminus to NP_302202.1|NC_002677 possible transcriptional regulator from Mycobacterium leprae (1106 aa). Also highly similar to several Mycobacterium tuberculosis putative transcriptional regulators e.g. Q1102|MTCY02B10_22 probable transcriptional regulatory protein (1159 aa), FASTA scores: opt: 702, E(): 8.3e-40, (50.6% identity in 247 aa overlap); MTV036_21; MTV008_44; MTCY02B10_23. Also shows similarity with several adenylate cyclases and hydrolases from other organisms. Q10551 No regulatory proteins Mb0915c NC_000962.3 Mycobrowser_v2 RBS 993847 993850 . + 0 Rv0892 Rv0892 Function unknown; probably involved in cellular metabolism. Probable monooxygenase Rv0892, (MTCY31.20), len: 495 aa. Probable monooxygenase, highly similar to others e.g. NP_250787.1|NC_002516 probable flavin-binding monooxygenase from Pseudomonas aeruginosa (491 aa); CAB59668.1|AL132674 monooxygenase from Streptomyces coelicolor (519 aa); P12015|CYMO_ACIS cyclohexanone monooxygenase from Acinetobacter sp. (542 aa), FASTA scores: opt: 489, E(): 6.8e-26, (30.3% identity in 492 aa overlap); etc. Also highly similar to Rv0565c, Rv3854c, Rv3083, etc from Mycobacterium tuberculosis. Has hydrophobic stretch at N-terminus. P64745 No intermediary metabolism and respiration MMAR_4641 Mb0916 NC_000962.3 Mycobrowser_v2 CDS 993853 995340 . + 0 Rv0892 Rv0892 Function unknown; probably involved in cellular metabolism. Probable monooxygenase Rv0892, (MTCY31.20), len: 495 aa. Probable monooxygenase, highly similar to others e.g. NP_250787.1|NC_002516 probable flavin-binding monooxygenase from Pseudomonas aeruginosa (491 aa); CAB59668.1|AL132674 monooxygenase from Streptomyces coelicolor (519 aa); P12015|CYMO_ACIS cyclohexanone monooxygenase from Acinetobacter sp. (542 aa), FASTA scores: opt: 489, E(): 6.8e-26, (30.3% identity in 492 aa overlap); etc. Also highly similar to Rv0565c, Rv3854c, Rv3083, etc from Mycobacterium tuberculosis. Has hydrophobic stretch at N-terminus. P64745 No intermediary metabolism and respiration MMAR_4641 Mb0916 NC_000962.3 Mycobrowser_v2 CDS 1024211 1024687 . + 0 Rv0918 Rv0918 Function unknown Conserved protein Rv0918, (MTCY21C12.12), len: 158 aa. Conserved protein, similar in part to Q50116 hypothetical protein from Mycobacterium leprae (44 aa), FASTA scores: opt: 132, E(): 0.0055, (65.6% identity in 32 aa overlap). Also some similarity in C-terminus with other hypothetical proteins e.g. NP_289961.1|NC_002655 hypothetical protein from Escherichia coli strain O157:H7 (94 aa); etc. O05910 No conserved hypotheticals ML2108 Mb0942 NC_000962.3 Mycobrowser_v2 CDS 995318 996295 . - 0 Rv0893c Rv0893c Possible methyltransferase Possible S-adenosylmethionine-dependent methyltransferase Rv0893c, (MTCY31.21c), len: 325 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), belongs in family with P96823|Rv0146|MTCI5.20 from Mycobacterium tuberculosis (310 aa), FASTA scores: opt: 784, E(): 0, (43.2% identity in 308 aa overlap); Rv0726c, Rv0731c, Rv3399, etc. Also shows some similarity with others e.g. SC9B5.10|T35930 hypothetical protein from Streptomyces coelicolor (303 aa); BSUB0008_141|Q45500 hypothetical 34.8 kDa protein from Bacillus subtilis (304 aa), FASTA scores: E(): 0.00033, (26.8% identity in 168 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P64747 No lipid metabolism Mb0917c NC_000962.3 Mycobrowser_v2 RBS 1027673 1027677 . + 0 Rv0922 Rv0922 Required for the transposition of the insertion element IS1535 Possible transposase Rv0922, (MTCY21C12.16), len: 550 aa. Possible transposase for IS1535, similar to many e.g. YX16_MYCTU|Q10809|MTCY274.16c from Mycobacterium tuberculosis (460 aa), FASTA scores: opt 939, E(): 0, (40.6% identity in 465 aa overlap); etc. O05914 No insertion seqs and phages Mb0946 NC_000962.3 Mycobrowser_v2 CDS 1027685 1029337 . + 0 Rv0922 Rv0922 Required for the transposition of the insertion element IS1535 Possible transposase Rv0922, (MTCY21C12.16), len: 550 aa. Possible transposase for IS1535, similar to many e.g. YX16_MYCTU|Q10809|MTCY274.16c from Mycobacterium tuberculosis (460 aa), FASTA scores: opt 939, E(): 0, (40.6% identity in 465 aa overlap); etc. O05914 No insertion seqs and phages Mb0946 NC_000962.3 Mycobrowser_v2 CDS 1142671 1143648 . + 0 Rv1021 Rv1021 Function unknown Conserved protein Rv1021, (MTCY10G2.28c), len: 325 aa. Conserved protein, similar to YBL1_STRCI|P33653 hypothetical 26.1 kDa protein from Streptomyces cacaoi (242 aa), FASTA scores: opt: 493, E(): 1.1e-23, (42.9% identity in 238 aa overlap). P96379 No conserved hypotheticals ML0253 MMAR_4464 MSMEG_5422 Mb1049 NC_000962.3 Mycobrowser_v2 CDS 996524 997705 . + 0 Rv0894 Rv0894 Thought to be involved in transcriptional mechanism. Possible transcriptional regulatory protein (possibly LuxR-family) Rv0894, (MTCY31.22), len: 393 aa. Possible regulatory protein, LuxR family, highly similar in part to NP_302202.1|NC_002677 possible transcriptional regulator from Mycobacterium leprae (1106 aa). Also similar to others e.g. CAB95788.1|AL359949 putative multi-domain regulatory protein from Streptomyces coelicolor (780 aa); NP_107293.1|NC_002678 transcriptional regulator from Mesorhizobium loti (903 aa); etc. Also similar to other regulatory proteins from Mycobacterium tuberculosis e.g. Rv2488c|MTV008_44 (1137 aa), FASTA score: (53.2% identity in 363 aa overlap); Rv1358|MTCY02B10_22 (1159 aa), FASTA score: (52.3% identity in 365 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P64749 No regulatory proteins Mb0918 NC_000962.3 Mycobrowser_v2 RBS 997764 997767 . + 0 Rv0895 Rv0895 May be involved in synthesis of triacylglycerol Possible triacylglycerol synthase (diacylglycerol acyltransferase) Rv0895, (MTCY31.23), len: 505 aa. Possible triacylglycerol synthase (See Daniel et al., 2004); member of family with: Rv3740c, Rv3734c, Rv1425, Rv1760, etc. Shows some similarity with NP_301898.1|NC_002677 conserved membrane protein from Mycobacterium leprae (491 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P67204 No lipid metabolism Mb0919 NC_000962.3 Mycobrowser_v2 CDS 997782 999299 . + 0 Rv0895 Rv0895 May be involved in synthesis of triacylglycerol Possible triacylglycerol synthase (diacylglycerol acyltransferase) Rv0895, (MTCY31.23), len: 505 aa. Possible triacylglycerol synthase (See Daniel et al., 2004); member of family with: Rv3740c, Rv3734c, Rv1425, Rv1760, etc. Shows some similarity with NP_301898.1|NC_002677 conserved membrane protein from Mycobacterium leprae (491 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P67204 No lipid metabolism Mb0919 NC_000962.3 Mycobrowser_v2 RBS 999461 999464 . + 0 Rv0896 gltA2 Involved in tricarboxylic acid cycle (KREBS cycle) [catalytic activity: citrate + CoA = acetyl-CoA + H2O + oxaloacetate]. Probable citrate synthase I GltA2 Rv0896, (MTCY31.24), len: 431 aa. Probable gltA2, citrate synthase 1, highly similar to O33066|NP_302405.1|NC_002677 citrate synthase 1 from Mycobacterium leprae (431 aa), FASTA scores: E(): 0, (91.0 identity in 431 aa overlap); and AAF04133.1|AF191033_1|AF191033 citrate synthase from Mycobacterium smegmatis (441 aa). Also highly similar to others e.g. AAF14286.1|AF181118_1|AF181118 citrate synthase from Streptomyces coelicolor (429 aa); P42457|CISY_CORGL citrate synthase from Corynebacterium glutamicum (437 aa), FASTA scores: opt: 1847, E(): 0, (63.0% identity in 433 aa overlap); etc. Also similar to two other Mycobacterium tuberculosis citrate synthases, Rv0889|MTCY31.17c|citA (373 aa), FASTA score: (29.2% identity in 274 aa overlap) and Rv1131|MTCY22G8.20|gltA1 (393 aa). Contains PS00480 Citrate synthase signature. Belongs to the citrate synthase family. Q10530 No intermediary metabolism and respiration ML2130 MMAR_4640 MSMEG_5672 Mb0920 NC_000962.3 Mycobrowser_v2 CDS 999472 1000767 . + 0 Rv0896 gltA2 Involved in tricarboxylic acid cycle (KREBS cycle) [catalytic activity: citrate + CoA = acetyl-CoA + H2O + oxaloacetate]. Probable citrate synthase I GltA2 Rv0896, (MTCY31.24), len: 431 aa. Probable gltA2, citrate synthase 1, highly similar to O33066|NP_302405.1|NC_002677 citrate synthase 1 from Mycobacterium leprae (431 aa), FASTA scores: E(): 0, (91.0 identity in 431 aa overlap); and AAF04133.1|AF191033_1|AF191033 citrate synthase from Mycobacterium smegmatis (441 aa). Also highly similar to others e.g. AAF14286.1|AF181118_1|AF181118 citrate synthase from Streptomyces coelicolor (429 aa); P42457|CISY_CORGL citrate synthase from Corynebacterium glutamicum (437 aa), FASTA scores: opt: 1847, E(): 0, (63.0% identity in 433 aa overlap); etc. Also similar to two other Mycobacterium tuberculosis citrate synthases, Rv0889|MTCY31.17c|citA (373 aa), FASTA score: (29.2% identity in 274 aa overlap) and Rv1131|MTCY22G8.20|gltA1 (393 aa). Contains PS00480 Citrate synthase signature. Belongs to the citrate synthase family. Q10530 No intermediary metabolism and respiration ML2130 MMAR_4640 MSMEG_5672 Mb0920 NC_000962.3 Mycobrowser_v2 RBS 1256122 1256124 . + 0 Rv0896 gltA2 Involved in tricarboxylic acid cycle (KREBS cycle) [catalytic activity: citrate + CoA = acetyl-CoA + H2O + oxaloacetate]. Probable citrate synthase I GltA2 Rv0896, (MTCY31.24), len: 431 aa. Probable gltA2, citrate synthase 1, highly similar to O33066|NP_302405.1|NC_002677 citrate synthase 1 from Mycobacterium leprae (431 aa), FASTA scores: E(): 0, (91.0 identity in 431 aa overlap); and AAF04133.1|AF191033_1|AF191033 citrate synthase from Mycobacterium smegmatis (441 aa). Also highly similar to others e.g. AAF14286.1|AF181118_1|AF181118 citrate synthase from Streptomyces coelicolor (429 aa); P42457|CISY_CORGL citrate synthase from Corynebacterium glutamicum (437 aa), FASTA scores: opt: 1847, E(): 0, (63.0% identity in 433 aa overlap); etc. Also similar to two other Mycobacterium tuberculosis citrate synthases, Rv0889|MTCY31.17c|citA (373 aa), FASTA score: (29.2% identity in 274 aa overlap) and Rv1131|MTCY22G8.20|gltA1 (393 aa). Contains PS00480 Citrate synthase signature. Belongs to the citrate synthase family. Q10530 No intermediary metabolism and respiration ML2130 MMAR_4640 MSMEG_5672 Mb0920 NC_000962.3 Mycobrowser_v2 CDS 1000808 1002415 . - 0 Rv0897c Rv0897c Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv0897c, (MTCY31.25c), len: 535 aa. Possible oxidoreductase, similar to various oxidoreductases from diverse organisms e.g. CAB94055.1|AL358672 putative oxidoreductase from Streptomyces coelicolor (540 aa); NP_147877.1|NC_000854 phytoene dehydrogenase from Aeropyrum pernix (549 aa); Q01671|CRTD_RHOSH methoxyneurosporene dehydrogenase from Rhodobacter sphaeroides (495 aa), FASTA scores: opt: 139, E(): 2.6e-06, (23.8% identity in 538 aa overlap); etc. Also similar to Rv1432, Rv2997, and Rv3829c from Mycobacterium tuberculosis. P64751 No intermediary metabolism and respiration ML2127 MMAR_4639 MSMEG_5667 Mb0921c NC_000962.3 Mycobrowser_v2 CDS 1002441 1002704 . - 0 Rv0898c Rv0898c Function unknown Conserved hypothetical protein Rv0898c, (MTCY31.26c), len: 87 aa. Conserved hypothetical protein, highly similar to CAC01589.1|AL391041 hypothetical protein from Streptomyces coelicolor (87 aa). Also shows some similarity to Rv0709|MTCY210.28|rpmC from Mycobacterium tuberculosis (77 aa), FASTA score: (28.8% identity in 73 aa overlap). P64753 No conserved hypotheticals MMAR_4638 MSMEG_5666 Mb0922c NC_000962.3 Mycobrowser_v2 RBS 1002801 1002804 . + 0 Rv0899 ompA The protein behaved as a porin of low specific activity. Structural protein that may protect the integrity of the bacterium. Outer membrane protein A OmpA Rv0899, (MTCY31.27), len: 326 aa. OmpA, outer membrane protein A (See Senaratne et al., 1998). C-terminal region similar to C-terminus of many members of the OmpA family of outer membrane proteins, e.g. NP_458280.1|NC_003198 putative outer membrane protein from Salmonella enterica subsp. enterica serovar Typhi (220); NP_418008.1|NC_000913 putative outer membrane protein from Escherichia coli strain K12 (219 aa), FASTA scores: opt: 296, E(): 2.2e-11, (45.3% identity in 117 aa overlap); NP_231844.1|NC_002505 outer membrane protein OmpA from Vibrio cholerae (321 aa); Q05146|OMPA_BORAV outer membrane protein A precursor from Bordetella avium (194 aa); etc. A signal peptide sequence probably exists at the N-terminus. N-terminal domain is necessary and sufficient for membrane translocation (See Alahari et al., 2007). Contains PS00044 Bacterial regulatory proteins, lysR family signature. Belongs to the OmpA family. Pore-forming activity is pH-dependent. P65593 No cell wall and cell processes ML2126 MMAR_4637 Mb0923 NC_000962.3 Mycobrowser_v2 CDS 1002812 1003792 . + 0 Rv0899 ompA The protein behaved as a porin of low specific activity. Structural protein that may protect the integrity of the bacterium. Outer membrane protein A OmpA Rv0899, (MTCY31.27), len: 326 aa. OmpA, outer membrane protein A (See Senaratne et al., 1998). C-terminal region similar to C-terminus of many members of the OmpA family of outer membrane proteins, e.g. NP_458280.1|NC_003198 putative outer membrane protein from Salmonella enterica subsp. enterica serovar Typhi (220); NP_418008.1|NC_000913 putative outer membrane protein from Escherichia coli strain K12 (219 aa), FASTA scores: opt: 296, E(): 2.2e-11, (45.3% identity in 117 aa overlap); NP_231844.1|NC_002505 outer membrane protein OmpA from Vibrio cholerae (321 aa); Q05146|OMPA_BORAV outer membrane protein A precursor from Bordetella avium (194 aa); etc. A signal peptide sequence probably exists at the N-terminus. N-terminal domain is necessary and sufficient for membrane translocation (See Alahari et al., 2007). Contains PS00044 Bacterial regulatory proteins, lysR family signature. Belongs to the OmpA family. Pore-forming activity is pH-dependent. P65593 No cell wall and cell processes ML2126 MMAR_4637 Mb0923 NC_000962.3 Mycobrowser_v2 CDS 1010136 1011734 . + 0 Rv0907 Rv0907 Function unknown, possibly involved in cell wall biosynthesis. Conserved protein Rv0907, (MTCY21C12.01), len: 532 aa. Conserved protein, possibly involved in cell wall biosynthesis: similar to many beta-lactamases, penicillin-binding proteins and hypothetical proteins e.g. NP_298910.1|NC_002488 beta-lactamase from Xylella fastidiosa (455 aa); Q06317|PBP4_NOCLA penicillin-binding protein 4 (PBP-4) (381 aa), FASTA scores: opt: 299, E(): 8.8e-05, (28.7% identity in 401 aa overlap); etc. N-terminus highly similar to AAA63047.1|U15184 hypothetical protein from Mycobacterium leprae (58 aa). Related to other putative esterases and penicillin binding proteins in Mycobacterium tuberculosis e.g. Rv1730c|MTCY04C12.15c (517 aa). O05900 No cell wall and cell processes ML2116 MMAR_4623 MSMEG_5637 Mb0931 NC_000962.3 Mycobrowser_v2 CDS 1004501 1005841 . - 0 Rv0902c prrB Sensor part of the two component regulatory system PRRA/PRRB. Thought to be involved in the environmental adaptation, specifically in an early phase of the intracellular growth. Two component sensor histidine kinase PrrB Rv0902c, (MTCY31.30c), len: 446 aa. PrrB, two-component sensor histidine kinase (see citations below), transmembrane protein, equivalent to MLCB57_26|NP_302403.1|NC_002677 sensor histidine kinase from Mycobacterium leprae (446 aa); and similar at C-termini to NP_301251.1|NC_002677 putative two-component system sensor kinase from Mycobacterium leprae (519 aa). C-terminus also similar to the C-termini of many sensor-like histidine kinase proteins e.g. P08336|CPXA_ECOLI|ECFB|SSD|EUP|B3911|Z5456|ECS4837 sensor protein from Escherichia coli strain K12 (457 aa), FASTA scores: opt: 364, E(): 1.7e-15, (27.1% identity in 398 aa overlap); CAB89748.1|AL354616 putative two-component histidine kinase from Streptomyces coelicolor (483 aa); CAB82845.1|AJ277081 putative histidine kinase from Amycolatopsis mediterranei (472 aa); etc. Also similar in part to Mycobacterium tuberculosis proteins Rv3764c (475 aa); and Rv0982 (504 aa). Thought to be induced at phagocytosis (see Graham & Clark-Curtiss 1999). P0A5Z8 No regulatory proteins ML2124 MMAR_4634 MSMEG_5663 Mb0926c NC_000962.3 Mycobrowser_v2 CDS 1005852 1006562 . - 0 Rv0903c prrA Transcriptional regulator part of the two component regulatory system PRRA/PRRB. Thought to be involved in the environmental adaptation, specifically in an early phase of the intracellular growth. Two component response transcriptional regulatory protein PrrA Rv0903c, (MTCY31.31c), len: 236 aa. PrrA, two-component response regulator (see citations below), equivalent to Z99494|MLCB57_27|NP_302402.1|NC_002677 two-component response regulator from Mycobacterium leprae (233 aa), FASTA scores: opt: 1414, E(): 0, (95.7% identity in 233 aa overlap); and similar to T45446 probable two-component response regulator from Mycobacterium leprae (253 aa). Also similar to many sensor-like histidine kinase proteins e.g. CAB88489.1|AL353816 putative two-component systen response regulator from Streptomyces coelicolor (248 aa); AAG36759.1|AF119221_1 |AF119221 response regulator from Corynebacterium glutamicum (232 aa); Q02540|COPR_PSESM transcriptional activator protein COPR from Pseudomonas syringae (pv. tomato) (227 aa), FASTA scores: opt: 600, E(): 0, (44.4% identity in 225 aa overlap); etc. Also similar to Rv0981 from Mycobacterium tuberculosis (230 aa), Rv3765c (234 aa), phoP (247 aa), etc. Thought to be induced at phagocytosis (see Graham & Clark-Curtiss 1999). P0A5Z6 No regulatory proteins ML2123 MMAR_4633 MSMEG_5662 Mb0927c NC_000962.3 Mycobrowser_v2 CDS 1006693 1008180 . - 0 Rv0904c accD3 This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA [catalytic activity ATP + acetyl-CoA + HCO(3)(-) = ADP + phosphate + malonyl-CoA]. Putative acetyl-coenzyme A carboxylase carboxyl transferase (subunit beta) AccD3 (accase beta chain) Rv0904c, (MTCY31.32c, MT0927), len: 495 aa. Putative accD3, acetyl-CoA carboxylase carboxyl transferase, beta subunit (carboxyltransferase subunit of acetyl-CoA carboxylase), highly similar in part to AAA63045.1|U15184 zinc finger protein from Mycobacterium leprae (201 aa). Also highly similar to others e.g. CAC42827.1|Y17592 putative carboxyltransferase subunit of acetyl-CoA carboxylase from Corynebacterium glutamicum (491 aa); CAB86110.1|AL163003 putative acetyl CoA carboxylase (alpha and beta subunits) from Streptomyces coelicolor (458 aa); Q54776|ACCD_SYNP7 acetyl-coenzyme A carboxylase carboxyl transferase subunit beta from Synechococcus sp. (305 aa); P12217|ACCD_MARPO acetyl-coenzyme A carboxylase carboxyl transferase subunit beta from Marchantia polymorpha (316 aa), FASTA scores: opt: 519, E():1.6e-24, (40.2% identity in 219 aa overlap); etc. Also similar to Rv3280, Rv2502c, etc from Mycobacterium tuberculosis. Belongs to the ACCD/PCCB family. P63405 No lipid metabolism ML2119 MMAR_4626 MSMEG_5642 Mb0928c NC_000962.3 Mycobrowser_v2 CDS 1008207 1008938 . + 0 Rv0905 echA6 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Possible enoyl-CoA hydratase EchA6 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv0905, (MTCY31.33), len: 243 aa. Possible echA6, enoyl-CoA hydratase, highly similar to ML15184|U15184 enoyl-CoA hydratase from Mycobacterium leprae (247 aa), FASTA score: (85.8% identity in 247 aa overlap). Also similar to many e.g. NP_250320.1|NC_002516 probable enoyl-CoA hydratase/isomerase from Pseudomonas aeruginosa (261 aa); NP_415911.1|NC_000913 putative enzyme from Escherichia coli strain K12 (255 aa); P24162|ECHH_RHOCA|FADB1 enoyl-CoA hydratase homolog from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (257 aa), FASTA scores: opt: 404, E():7.8e-21, (37.3% identity in 249 aa overlap); etc. P64014 No lipid metabolism ML2118 MMAR_4625 MSMEG_5639 Mb0929 NC_000962.3 Mycobrowser_v2 CDS 1011731 1014124 . + 0 Rv0908 ctpE Metal cation-transporting ATPase; possibly catalyzes the transport of an undetermined metal cation with the hydrolysis of ATP [catalytic activity: ATP + H(2)O + undetermined metal cation(in) = ADP + phosphate + undetermined metal cation(out)]. Probable metal cation transporter ATPase P-type CtpE Rv0908, (MTCY21C12.02), len: 797 aa. Probable ctpE, metal cation-transporting ATPase P-type, transmembrane protein, E1-E2 family, highly similar to many e.g. AB93406.1|AL357524 putative integral membrane ATPase from Streptomyces coelicolor (802 aa); NP_346063.1|NC_003028 cation-transporting ATPase (E1-E2 family) from Streptococcus pneumoniae (778 aa); P37278|ATCL_SYNP7|PACL cation-transporting atpase from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (926 aa), FASTA scores: opt: 257, E(): 4.8e-33, (27.7% identity in 905 aa overlap); etc. Contains E1-E2 ATPases phosphorylation site (PS00154). Belongs to the cation transport ATPases family (E1-E2 ATPases). P0A504 No cell wall and cell processes ML2115 MMAR_4622 MSMEG_5636 Mb0932 NC_000962.3 Mycobrowser_v2 CDS 1014681 1014860 . + 0 Rv0909 Rv0909 Function unknown Conserved hypothetical protein Rv0909, (MTCY21C12.03), len: 59 aa. Conserved hypothetical protein, equivalent to NP_302399.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (56 aa). Also some similarity with AL022268|SC4H2_10c hypothetical protein from Streptomyces coelicolor (97 aa), FASTA scores: opt: 106, E(): 0.13, (43.2% identity in 37 aa overlap). O05901 No conserved hypotheticals ML2114 MMAR_4620 MSMEG_5635 Mb0933 NC_000962.3 Mycobrowser_v2 CDS 1014866 1015300 . + 0 Rv0910 Rv0910 Function unknown Conserved hypothetical protein Rv0910, (MTCY21C12.04), len: 144 aa. Conserved hypothetical protein, equivalent to NP_302398.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (181 aa), FASTA scores: opt: 820, E(): 0, (83.9% identity in 143 aa overlap). Also similar to Rv1546|MTCY48.19c hypothetical protein from Mycobacterium tuberculosis (143 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O05902 No conserved hypotheticals ML2113 MMAR_4619 MSMEG_5634 Mb0934 NC_000962.3 Mycobrowser_v2 RBS 1015386 1015391 . + 0 Rv0911 Rv0911 Function unknown Conserved protein Rv0911, (MTCY21C12.05), len: 257 aa. Conserved protein, showing similarity with hydroxylases and hypothetical proteins e.g. T35325 probable hydroxylase from Streptomyces coelicolor (265 aa); Q54242 hypothetical protein from Streptomyces, FASTA scores: opt: 372, E(): 8.8e-18, (32.0% identity in 256 aa overlap); AAD04716.1|U77891 doxorubicin biosynthesis enzyme DnrV from Streptomyces peucetius (275 aa); AAA63051.1|U15184 hypothetical protein from Mycobacterium leprae (94 aa); etc. Also similar to Rv0577 hypothetical protein from Mycobacterium tuberculosis (261 aa). O05903 No conserved hypotheticals ML2112 MMAR_4617 MSMEG_5616 Mb0935 NC_000962.3 Mycobrowser_v2 CDS 1015398 1016171 . + 0 Rv0911 Rv0911 Function unknown Conserved protein Rv0911, (MTCY21C12.05), len: 257 aa. Conserved protein, showing similarity with hydroxylases and hypothetical proteins e.g. T35325 probable hydroxylase from Streptomyces coelicolor (265 aa); Q54242 hypothetical protein from Streptomyces, FASTA scores: opt: 372, E(): 8.8e-18, (32.0% identity in 256 aa overlap); AAD04716.1|U77891 doxorubicin biosynthesis enzyme DnrV from Streptomyces peucetius (275 aa); AAA63051.1|U15184 hypothetical protein from Mycobacterium leprae (94 aa); etc. Also similar to Rv0577 hypothetical protein from Mycobacterium tuberculosis (261 aa). O05903 No conserved hypotheticals ML2112 MMAR_4617 MSMEG_5616 Mb0935 NC_000962.3 Mycobrowser_v2 CDS 1016236 1016685 . + 0 Rv0912 Rv0912 Unknown Probable conserved transmembrane protein Rv0912, (MTCY21C12.06), len: 149 aa. Probable conserved transmembrane protein, equivalent to Q50121|NP_302397.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (144 aa), FASTA scores: opt: 677, E(): 6.9e-38, (69.5% identity in 141 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O05904 No cell wall and cell processes ML2111 MMAR_4616 MSMEG_5613 Mb0936 NC_000962.3 Mycobrowser_v2 CDS 1017217 1018725 . - 0 Rv0913c Rv0913c Function unknown; probably involved in cellular metabolism. Possible dioxygenase Rv0913c, (MTCY21C12.07c), len: 502 aa. Possible dioxygenase, showing similarity with others e.g. AAK38744.1|AY029525 carotenoid 9,10-9',10' cleavage dioxygenase from Phaseolus vulgaris (543 aa); CAB56138.1|AL117669 putative dioxygenase from Streptomyces coelicolor (503 aa); AAK06796.1|AF324838_15|AF324838 putative dioxygenase SimC5 from Streptomyces antibioticus (456 aa); Q53353|S65040 lignostilbene-alpha,beta-dioxygenase from Pseudomonas paucimobilis (485 aa), FASTA scores: opt: 310, E(): 3.4e-20, (28.9% identity in 495 aa overlap); etc. Also some similarity with Rv0654|MTCI376.22 probable dioxygenase from Mycobacterium tuberculosis (501 aa). O05905 No intermediary metabolism and respiration ML2110 MMAR_4614 MSMEG_5609 Mb0937c NC_000962.3 Mycobrowser_v2 CDS 1018727 1019965 . - 0 Rv0914c Rv0914c Thought to be involved in degradative pathways such as fatty acid BETA_OXIDATION. Possible lipid carrier protein or keto acyl-CoA thiolase Rv0914c, (MTCY21C12.08c), len: 412 aa. Possible lipid carrier protein or keto acyl-CoA thiolase, highly similar to NP_421905.1|NC_002696 thiolase family protein from Caulobacter crescentus (407 aa); and similar to others e.g. NP_107896.1|NC_002678 3-ketoacyl-CoA thiolase from Mesorhizobium loti (392 aa); NP_385796.1|NC_003047 putative 3-ketoacyl-CoA thiolase protein from Sinorhizobium meliloti (389 aa); NP_275932.1|NC_000916 lipid-transfer protein (sterol or nonspecific) from Methanothermobacter thermautotrophicus (383 aa); AB55378.1|AL117263 possible 3-ketoacyl-CoA thiolase from Leishmania major (441 aa), FASTA scores: opt: 547, E(): 3.1e-26, (31.0% identity in 435 aa overlap); etc. Also similar to Rv2790c, Rv1627c, Rv0244, etc from Mycobacterium tuberculosis. Could belong to the thiolase family. O05906 No lipid metabolism ML2109 MMAR_4613 MSMEG_5608 Mb0938c NC_000962.3 Mycobrowser_v2 RBS 1018734 1018737 . - 0 Rv0914c Rv0914c Thought to be involved in degradative pathways such as fatty acid BETA_OXIDATION. Possible lipid carrier protein or keto acyl-CoA thiolase Rv0914c, (MTCY21C12.08c), len: 412 aa. Possible lipid carrier protein or keto acyl-CoA thiolase, highly similar to NP_421905.1|NC_002696 thiolase family protein from Caulobacter crescentus (407 aa); and similar to others e.g. NP_107896.1|NC_002678 3-ketoacyl-CoA thiolase from Mesorhizobium loti (392 aa); NP_385796.1|NC_003047 putative 3-ketoacyl-CoA thiolase protein from Sinorhizobium meliloti (389 aa); NP_275932.1|NC_000916 lipid-transfer protein (sterol or nonspecific) from Methanothermobacter thermautotrophicus (383 aa); AB55378.1|AL117263 possible 3-ketoacyl-CoA thiolase from Leishmania major (441 aa), FASTA scores: opt: 547, E(): 3.1e-26, (31.0% identity in 435 aa overlap); etc. Also similar to Rv2790c, Rv1627c, Rv0244, etc from Mycobacterium tuberculosis. Could belong to the thiolase family. O05906 No lipid metabolism ML2109 MMAR_4613 MSMEG_5608 Mb0938c NC_000962.3 Mycobrowser_v2 CDS 1020058 1021329 . - 0 Rv0915c PPE14 Function unknown. Possibly a protective antigen involved with the early control of infection. PPE family protein PPE14 Rv0915c, (MTCY21C12.09c), len: 423 aa. PPE14 (alternate gene name: MTB41). Member of the Mycobacterium tuberculosis PPE family (see citation below), highly similar to many e.g. Rv1807 from Mycobacterium tuberculosis (403 aa), FASTA scores: opt: 966, E(): 4.4e-30, (45.7% identity in 392 aa overlap); etc. Contains PS00626 Regulator of chromosome condensation (RCC1) signature 2. Q79FV1 No PE/PPE Mb0939c NC_000962.3 Mycobrowser_v2 RBS 1021339 1021345 . - 0 Rv0915c PPE14 Function unknown. Possibly a protective antigen involved with the early control of infection. PPE family protein PPE14 Rv0915c, (MTCY21C12.09c), len: 423 aa. PPE14 (alternate gene name: MTB41). Member of the Mycobacterium tuberculosis PPE family (see citation below), highly similar to many e.g. Rv1807 from Mycobacterium tuberculosis (403 aa), FASTA scores: opt: 966, E(): 4.4e-30, (45.7% identity in 392 aa overlap); etc. Contains PS00626 Regulator of chromosome condensation (RCC1) signature 2. Q79FV1 No PE/PPE Mb0939c NC_000962.3 Mycobrowser_v2 CDS 1021344 1021643 . - 0 Rv0916c PE7 Function unknown PE family protein PE7 Rv0916c, (MTCY21C12.10c), len: 99 aa. PE7 (alternate gene name: MTB10). Member of the Mycobacterium tuberculosis PE family (see citations below), similar to many e.g. Rv1788 from Mycobacterium tuberculosis (99 aa), FASTA scores: opt: 321, E(): 1.3e-11, (53.5% identity in 99 aa overlap); etc. Q7D937 No PE/PPE Mb0940c NC_000962.3 Mycobrowser_v2 RBS 1021650 1021654 . - 0 Rv0916c PE7 Function unknown PE family protein PE7 Rv0916c, (MTCY21C12.10c), len: 99 aa. PE7 (alternate gene name: MTB10). Member of the Mycobacterium tuberculosis PE family (see citations below), similar to many e.g. Rv1788 from Mycobacterium tuberculosis (99 aa), FASTA scores: opt: 321, E(): 1.3e-11, (53.5% identity in 99 aa overlap); etc. Q7D937 No PE/PPE Mb0940c NC_000962.3 Mycobrowser_v2 RBS 1022074 1022077 . + 0 Rv0917 betP High-affinity uptake of glycine betaine. Supposedly responsible for the translocation of the substrate across the membrane. Possible glycine betaine transport integral membrane protein BetP Rv0917, (MTCY21C12.11), len: 593 aa. Possible betP, glycine betaine transporter, integral membrane protein, highly similar to many transporters, mainly glycine betaine transporters, e.g. P54582|BETP_CORGL glycine betaine transporter from Corynebacterium glutamicum (Brevibacterium flavum) (595 aa), FASTA scores: opt: 1367, E(): 0, (42.7% identity in 504 aa overlap); T35264 probable BccT family transporter from Streptomyces coelicolor (578 aa); NP_243511.1|NC_002570 glycine betaine transporter from Bacillus halodurans (504 aa); NP_439848.1|NC_000907 high-affinity choline transport protein (betT) from Haemophilus influenzae (669 aa); etc. Seems to belong to the BCCT (TC 2.33) family of transporters. P63695 No cell wall and cell processes MMAR_2913 Mb0941 NC_000962.3 Mycobrowser_v2 CDS 1022087 1023868 . + 0 Rv0917 betP High-affinity uptake of glycine betaine. Supposedly responsible for the translocation of the substrate across the membrane. Possible glycine betaine transport integral membrane protein BetP Rv0917, (MTCY21C12.11), len: 593 aa. Possible betP, glycine betaine transporter, integral membrane protein, highly similar to many transporters, mainly glycine betaine transporters, e.g. P54582|BETP_CORGL glycine betaine transporter from Corynebacterium glutamicum (Brevibacterium flavum) (595 aa), FASTA scores: opt: 1367, E(): 0, (42.7% identity in 504 aa overlap); T35264 probable BccT family transporter from Streptomyces coelicolor (578 aa); NP_243511.1|NC_002570 glycine betaine transporter from Bacillus halodurans (504 aa); NP_439848.1|NC_000907 high-affinity choline transport protein (betT) from Haemophilus influenzae (669 aa); etc. Seems to belong to the BCCT (TC 2.33) family of transporters. P63695 No cell wall and cell processes MMAR_2913 Mb0941 NC_000962.3 Mycobrowser_v2 CDS 2204866 2205273 . - 0 Rv1962c vapC35 Unknown Possible toxin VapC35. Contains PIN domain. Rv1962c, (MTCY09F9.02), len: 135 aa. Possible vapC35, toxin, part of toxin-antitoxin (TA) operon with Rv1962A, contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. Rv3408|MTCY78.20c (133 aa) (36.2% identity in 138 aa overlap); and Rv3384c (130 aa) (43.1% identity in 130 aa overlap) P95252 No virulence, detoxification, adaptation Mb1997c NC_000962.3 Mycobrowser_v2 CDS 1064114 1064962 . - 0 Rv0953c Rv0953c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0953c, (MTCY10D7.21), len: 282 aa. Possible oxidoreductase, equivalent to CAA48222.1|X68102 hypothetical protein from Mycobacterium avium subsp. paratuberculosis (166 aa). Similar to several hypothetical proteins and oxidoreductases e.g. AAK38097.1|AF323606_3|AF323606 putative F420-dependent dehydrogenase from Rhodococcus erythropolis (295 aa); NP_070025.1|NC_000917 N5,N10-methylenetetrahydromethanopterin reductase (mer-2) from Archaeoglobus fulgidus (348 aa); etc. Also similar to several hypothetical proteins and oxidoreductases from Mycobacterium tuberculosis e.g. Rv2161c|O06216|Z95388|MTCY270.07 (288 aa), FASTA scores: opt: 633, E(): 0, (40.4% identity in 277 aa overlap), Rv3079c (275 aa), Rv0791c (347 aa), etc. Contains PS00201 Flavodoxin signature. P64769 No intermediary metabolism and respiration ML0157 MMAR_4546 MSMEG_5520 Mb0978c NC_000962.3 Mycobrowser_v2 CDS 1030578 1031864 . - 0 Rv0924c mntH H(+)-stimulated, highly selective, divalent cation uptake system. Responsible for the translocation of the divalent metal across the membrane. Divalent cation-transport integral membrane protein MntH (BRAMP) (MRAMP) Rv0924c, (MTCY21C12.18c), len: 428 aa. MntH (alternative gene name: Nramp, Mramp), H+-dependent divalent cation-transport integral membrane protein (see citations below), equivalent to O69443|MNTH_MYCBO probable manganese transport protein MNTH (BRAMP) from Mycobacterium bovis (415 aa); and NP_302396.1|NC_002677 probable manganese transport protein from Mycobacterium leprae (426 aa). Also similar (but longer 51 aa in N-terminus) to AAA63075.1|U15184 SMF2 protein from Mycobacterium leprae (377 aa), FASTA scores: opt: 1780, E(): 0, (74.5% identity in 376 aa overlap). Also similar to many orthologues of the eukaryotic Nramp (natural resistance-associated macrophage protein), also known as mntH, e.g. NP_456951.1|NC_003198 manganese transport protein MntH from Salmonella enterica subsp. enterica serovar Typhi (413 aa); etc. Belongs to the NRAMP family. O05916 No cell wall and cell processes ML2098 MMAR_4584 MSMEG_5589 Mb0948c NC_000962.3 Mycobrowser_v2 CDS 1031896 1032633 . - 0 Rv0925c Rv0925c Function unknown Conserved protein Rv0925c, (MTCY21C12.19c), len: 245 aa. Conserved protein, similar to AL132991|SCF55_19 hypothetical protein from Streptomyces coelicolor (197 aa), FASTA scores: opt: 459, E(): 1.2e-23, (39.3% identity in 201 aa overlap). O05917 No conserved hypotheticals MMAR_4583 Mb0948c NC_000962.3 Mycobrowser_v2 CDS 1032710 1033786 . - 0 Rv0926c Rv0926c Function unknown Conserved hypothetical protein Rv0926c, (MTCY21C12.20c), len: 358 aa. Conserved hypothetical protein, similar to Rv1059 conserved hypothetical protein from Mycobacterium tuberculosis (354 aa). Also shows some similarity to AF170923|AF170923_3 dihydrodipicolinate reductase from Mastigocladus laminosus (278 aa), FASTA scores: opt: 170, E(): 0.00088, (25.7% identity in 276 aa overlap). O05918 No conserved hypotheticals ML2097 MMAR_4582 MSMEG_5586 Mb0949c NC_000962.3 Mycobrowser_v2 CDS 1033840 1034631 . - 0 Rv0927c Rv0927c Function unknown; possibly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv0927c, (MTCY21C12.21c), len: 263 aa. Probable short-chain dehydrogenase/reductase, similar to various dehydrogenases/reductases, notably 7-alpha-hydroxysteroid dehydrogenases and glucose 1-dehydrogenases e.g. P25529|HDHA_ECOLI 7-alpha-hydroxysteroid dehydrogenase from Escherichia coli (255 aa), FASTA scores: opt: 551, E(): 1e-26, (39.5% identity in 248 aa overlap); NP_252778.1|NC_002516 probable short-chain dehydrogenase from Pseudomonas aeruginosa (253 aa); AAC44307.1|U59433 3-ketoacyl-acyl carrier protein reductase from Bacillus subtilis (246 aa); etc. Also similar to other dehydrogenases from Mycobacterium tuberculosis e.g. MTCY09F9.36, E():1.4e-18; MTCY369.14, E():8e-17; MTCY02B10.14, E():2.5e-14; MTCY09F9.23c, E():1.5e-13; MTCY03C7.07, E():1.9e-13. Contains PS00061 Short-chain dehydrogenases/reductases family signature, and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the short-chain dehydrogenases/reductases (SDR) family. O05919 No intermediary metabolism and respiration ML2096 MMAR_4581 MSMEG_5584 Mb0950c NC_000962.3 Mycobrowser_v2 RBS 1034637 1034640 . - 0 Rv0927c Rv0927c Function unknown; possibly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv0927c, (MTCY21C12.21c), len: 263 aa. Probable short-chain dehydrogenase/reductase, similar to various dehydrogenases/reductases, notably 7-alpha-hydroxysteroid dehydrogenases and glucose 1-dehydrogenases e.g. P25529|HDHA_ECOLI 7-alpha-hydroxysteroid dehydrogenase from Escherichia coli (255 aa), FASTA scores: opt: 551, E(): 1e-26, (39.5% identity in 248 aa overlap); NP_252778.1|NC_002516 probable short-chain dehydrogenase from Pseudomonas aeruginosa (253 aa); AAC44307.1|U59433 3-ketoacyl-acyl carrier protein reductase from Bacillus subtilis (246 aa); etc. Also similar to other dehydrogenases from Mycobacterium tuberculosis e.g. MTCY09F9.36, E():1.4e-18; MTCY369.14, E():8e-17; MTCY02B10.14, E():2.5e-14; MTCY09F9.23c, E():1.5e-13; MTCY03C7.07, E():1.9e-13. Contains PS00061 Short-chain dehydrogenases/reductases family signature, and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the short-chain dehydrogenases/reductases (SDR) family. O05919 No intermediary metabolism and respiration ML2096 MMAR_4581 MSMEG_5584 Mb0950c NC_000962.3 Mycobrowser_v2 RBS 1034893 1034896 . + 0 Rv0928 pstS3 Involved in active transport of inorganic phosphate across the membrane (import). This is one of the proteins required for binding-protein-mediated phosphate transport. Periplasmic phosphate-binding lipoprotein PstS3 (PBP-3) (PstS3) (PHOS1) Rv0928, (MTCY21C12.22), len: 370 aa. PstS3 (previously known as phoS2), phosphate-binding lipoprotein component of inorganic phosphate transport system (see citations below), highly similar to others from Mycobacterium leprae e.g. Q50099|PSTS3|PHOS1 phosphate-binding protein 3 precursor (328 aa), FASTA scores: opt: 1772, E(): 0, (79.6% identity in 328 aa overlap); and highly similar to others e.g. AAF74819.1|AF137360_1|AF137360 periplasmic phosphate permease from Mycobacterium avium (369 aa). Also highly similar to Rv0932c|MTCY08D9.07|pstS2 phosphate-binding periplasmic lipoprotein (370 aa); and Rv0934|pstS1 phosphate-binding periplasmic lipoprotein (374 aa) from Mycobacterium tuberculosis (Mycobacterium tuberculosis seems to have three PstS-like proteins, others being Rv0932c and Rv0934c). Contains lipoprotein signature (PS00013) at N-terminus. Belongs to family of phosphate receptors for bacterial ABC-type lipoprotein transporters. P0A5Y2 No cell wall and cell processes ML2095 MMAR_4580 Mb0951 NC_000962.3 Mycobrowser_v2 CDS 1034903 1036015 . + 0 Rv0928 pstS3 Involved in active transport of inorganic phosphate across the membrane (import). This is one of the proteins required for binding-protein-mediated phosphate transport. Periplasmic phosphate-binding lipoprotein PstS3 (PBP-3) (PstS3) (PHOS1) Rv0928, (MTCY21C12.22), len: 370 aa. PstS3 (previously known as phoS2), phosphate-binding lipoprotein component of inorganic phosphate transport system (see citations below), highly similar to others from Mycobacterium leprae e.g. Q50099|PSTS3|PHOS1 phosphate-binding protein 3 precursor (328 aa), FASTA scores: opt: 1772, E(): 0, (79.6% identity in 328 aa overlap); and highly similar to others e.g. AAF74819.1|AF137360_1|AF137360 periplasmic phosphate permease from Mycobacterium avium (369 aa). Also highly similar to Rv0932c|MTCY08D9.07|pstS2 phosphate-binding periplasmic lipoprotein (370 aa); and Rv0934|pstS1 phosphate-binding periplasmic lipoprotein (374 aa) from Mycobacterium tuberculosis (Mycobacterium tuberculosis seems to have three PstS-like proteins, others being Rv0932c and Rv0934c). Contains lipoprotein signature (PS00013) at N-terminus. Belongs to family of phosphate receptors for bacterial ABC-type lipoprotein transporters. P0A5Y2 No cell wall and cell processes ML2095 MMAR_4580 Mb0951 NC_000962.3 Mycobrowser_v2 RBS 1042105 1042108 . + 0 Rv0928 pstS3 Involved in active transport of inorganic phosphate across the membrane (import). This is one of the proteins required for binding-protein-mediated phosphate transport. Periplasmic phosphate-binding lipoprotein PstS3 (PBP-3) (PstS3) (PHOS1) Rv0928, (MTCY21C12.22), len: 370 aa. PstS3 (previously known as phoS2), phosphate-binding lipoprotein component of inorganic phosphate transport system (see citations below), highly similar to others from Mycobacterium leprae e.g. Q50099|PSTS3|PHOS1 phosphate-binding protein 3 precursor (328 aa), FASTA scores: opt: 1772, E(): 0, (79.6% identity in 328 aa overlap); and highly similar to others e.g. AAF74819.1|AF137360_1|AF137360 periplasmic phosphate permease from Mycobacterium avium (369 aa). Also highly similar to Rv0932c|MTCY08D9.07|pstS2 phosphate-binding periplasmic lipoprotein (370 aa); and Rv0934|pstS1 phosphate-binding periplasmic lipoprotein (374 aa) from Mycobacterium tuberculosis (Mycobacterium tuberculosis seems to have three PstS-like proteins, others being Rv0932c and Rv0934c). Contains lipoprotein signature (PS00013) at N-terminus. Belongs to family of phosphate receptors for bacterial ABC-type lipoprotein transporters. P0A5Y2 No cell wall and cell processes ML2095 MMAR_4580 Mb0951 NC_000962.3 Mycobrowser_v2 CDS 1076196 1076678 . - 0 Rv0964c Rv0964c Unknown Hypothetical protein Rv0964c, (MTCY10D7.10), len: 160 aa. Hypothetical unknown protein. Equivalent to AAK45241.1 from Mycobacterium tuberculosis strain CDC1551 (138 aa) but longer 22 aa. P71546 No conserved hypotheticals Mb0989c NC_000962.3 Mycobrowser_v2 RBS 1036019 1036022 . + 0 Rv0929 pstC2 Involved in active transport of inorganic phosphate across the membrane (import); responsible for the translocation of the substrate across the membrane. This is one of the proteins required for binding-protein-mediated phosphate transport. Phosphate-transport integral membrane ABC transporter PstC2 Rv0929, (MTCY21C12.23), len: 324 aa. PstC2, phosphate-transport integral membrane ABC transporter (see citations below), highly similar to others e.g. NP_302394.1|NC_002677 membrane-bound component of phosphate transport from Mycobacterium leprae (319 aa); CAB88474.1|AL353816 phosphate ABC transport system permease protein from Streptomyces coelicolor (336 aa); NP_290359.1| NC_002655 high-affinity phosphate-specific transport system (cytoplasmic membrane component) from Escherichia coli strain O157:H7 (319 aa); etc. Also similar to Rv935|MTCY08D9.04c|PSTC1 probable transmembrane ABC transporter component of phosphate uptake system from Mycobacterium tuberculosis (338 aa). Contains binding-protein-dependent transport systems inner membrane component signature (PS00402). P0A630 No cell wall and cell processes ML2094 MMAR_4579 Mb0952 NC_000962.3 Mycobrowser_v2 CDS 1036028 1037002 . + 0 Rv0929 pstC2 Involved in active transport of inorganic phosphate across the membrane (import); responsible for the translocation of the substrate across the membrane. This is one of the proteins required for binding-protein-mediated phosphate transport. Phosphate-transport integral membrane ABC transporter PstC2 Rv0929, (MTCY21C12.23), len: 324 aa. PstC2, phosphate-transport integral membrane ABC transporter (see citations below), highly similar to others e.g. NP_302394.1|NC_002677 membrane-bound component of phosphate transport from Mycobacterium leprae (319 aa); CAB88474.1|AL353816 phosphate ABC transport system permease protein from Streptomyces coelicolor (336 aa); NP_290359.1| NC_002655 high-affinity phosphate-specific transport system (cytoplasmic membrane component) from Escherichia coli strain O157:H7 (319 aa); etc. Also similar to Rv935|MTCY08D9.04c|PSTC1 probable transmembrane ABC transporter component of phosphate uptake system from Mycobacterium tuberculosis (338 aa). Contains binding-protein-dependent transport systems inner membrane component signature (PS00402). P0A630 No cell wall and cell processes ML2094 MMAR_4579 Mb0952 NC_000962.3 Mycobrowser_v2 RBS 1043294 1043297 . + 0 Rv0929 pstC2 Involved in active transport of inorganic phosphate across the membrane (import); responsible for the translocation of the substrate across the membrane. This is one of the proteins required for binding-protein-mediated phosphate transport. Phosphate-transport integral membrane ABC transporter PstC2 Rv0929, (MTCY21C12.23), len: 324 aa. PstC2, phosphate-transport integral membrane ABC transporter (see citations below), highly similar to others e.g. NP_302394.1|NC_002677 membrane-bound component of phosphate transport from Mycobacterium leprae (319 aa); CAB88474.1|AL353816 phosphate ABC transport system permease protein from Streptomyces coelicolor (336 aa); NP_290359.1| NC_002655 high-affinity phosphate-specific transport system (cytoplasmic membrane component) from Escherichia coli strain O157:H7 (319 aa); etc. Also similar to Rv935|MTCY08D9.04c|PSTC1 probable transmembrane ABC transporter component of phosphate uptake system from Mycobacterium tuberculosis (338 aa). Contains binding-protein-dependent transport systems inner membrane component signature (PS00402). P0A630 No cell wall and cell processes ML2094 MMAR_4579 Mb0952 NC_000962.3 Mycobrowser_v2 RBS 1036988 1036991 . + 0 Rv0930 pstA1 Involved in active transport of inorganic phosphate across the membrane (import); responsible for the translocation of the substrate across the membrane. This is one of the proteins required for binding-protein-mediated phosphate transport. Probable phosphate-transport integral membrane ABC transporter PstA1 Rv0930, (MTCY21C12.24), len: 308 aa. Probable pstA1, phosphate-transport integral membrane ABC transporter (see citation below), highly similar to others e.g. NP_302393.1|NC_002677 membrane-bound component of phosphate transport from Mycobacterium leprae (304 aa); CAB88473.1|AL353816 phosphate ABC transport system permease protein from Streptomyces coelicolor (354 aa) (N-terminus longer); NP_312689.1|NC_002695 phosphate transport system permease protein PstA from Escherichia coli strain O157:H7 (296 aa); etc. Also similar to Rv0936|MTCY08D9.03c|PSTA2 probable transmembrane ABC transporter component of phosphate uptake system from Mycobacterium tuberculosis (301 aa). O86345 No cell wall and cell processes ML2093 Mb0953 NC_000962.3 Mycobrowser_v2 CDS 1036999 1037925 . + 0 Rv0930 pstA1 Involved in active transport of inorganic phosphate across the membrane (import); responsible for the translocation of the substrate across the membrane. This is one of the proteins required for binding-protein-mediated phosphate transport. Probable phosphate-transport integral membrane ABC transporter PstA1 Rv0930, (MTCY21C12.24), len: 308 aa. Probable pstA1, phosphate-transport integral membrane ABC transporter (see citation below), highly similar to others e.g. NP_302393.1|NC_002677 membrane-bound component of phosphate transport from Mycobacterium leprae (304 aa); CAB88473.1|AL353816 phosphate ABC transport system permease protein from Streptomyces coelicolor (354 aa) (N-terminus longer); NP_312689.1|NC_002695 phosphate transport system permease protein PstA from Escherichia coli strain O157:H7 (296 aa); etc. Also similar to Rv0936|MTCY08D9.03c|PSTA2 probable transmembrane ABC transporter component of phosphate uptake system from Mycobacterium tuberculosis (301 aa). O86345 No cell wall and cell processes ML2093 Mb0953 NC_000962.3 Mycobrowser_v2 CDS 1037920 1039914 . - 0 Rv0931c pknD Involved in signal transduction (via phosphorylation). Thought to regulate phosphate transport. Can phosphorylate the peptide substrate myelin basic protein (MBP) at serine and threonine residues. Can be autophosphorylated on threonine residues [catalytic activity: ATP + a protein = ADP + a phosphoprotein]. Transmembrane serine/threonine-protein kinase D PknD (protein kinase D) (STPK D) Rv0931c, (MTCY08D9.08), len: 664 aa. PknD (alternate gene name: mbk), transmembrane serine/threonine protein kinase (see citations below), equivalent to CAB62227.1|AJ250200 putative serine/threonine protein kinase from Mycobacterium bovis BCG (291 aa); and highly similar in N-terminus to P54744|PKNB_MYCLE probable serine/threonine-specific protein kinase from Mycobacterium leprae (622 aa). Also highly similar to others, particularly in N-terminal half e.g. NP_243370.1|NC_002570 serine/threonine protein kinase from Bacillus halodurans (664 aa); NP_268044.1|NC_002662 serine/threonine protein kinase from Lactococcus lactis (627 aa); etc. Also highly similar to other serine/threonine protein kinases from Mycobacterium tuberculosis e.g. pknH (626 aa), FASTA scores: opt: 1398, E: 0, (49.3% identity in 540 aa overlap); pknE (566 aa); pknB (626 aa); Rv3524 (343 aa); etc. Contains Hank's kinase subdomain. Contains two transmembrane segments, which flank a highly repetitive region, suggesting a receptor-like anchoring. Belongs to the Ser/Thr family of protein kinases. Experimental studies show evidence of auto-phosphorylation on a serine residue. Appears to be co-transcribed with Rv0932c|pstS2. O05871 No regulatory proteins ML0743 MMAR_4577 Mb0954c,Mb0955c NC_000962.3 Mycobrowser_v2 RBS 1039925 1039928 . - 0 Rv0931c pknD Involved in signal transduction (via phosphorylation). Thought to regulate phosphate transport. Can phosphorylate the peptide substrate myelin basic protein (MBP) at serine and threonine residues. Can be autophosphorylated on threonine residues [catalytic activity: ATP + a protein = ADP + a phosphoprotein]. Transmembrane serine/threonine-protein kinase D PknD (protein kinase D) (STPK D) Rv0931c, (MTCY08D9.08), len: 664 aa. PknD (alternate gene name: mbk), transmembrane serine/threonine protein kinase (see citations below), equivalent to CAB62227.1|AJ250200 putative serine/threonine protein kinase from Mycobacterium bovis BCG (291 aa); and highly similar in N-terminus to P54744|PKNB_MYCLE probable serine/threonine-specific protein kinase from Mycobacterium leprae (622 aa). Also highly similar to others, particularly in N-terminal half e.g. NP_243370.1|NC_002570 serine/threonine protein kinase from Bacillus halodurans (664 aa); NP_268044.1|NC_002662 serine/threonine protein kinase from Lactococcus lactis (627 aa); etc. Also highly similar to other serine/threonine protein kinases from Mycobacterium tuberculosis e.g. pknH (626 aa), FASTA scores: opt: 1398, E: 0, (49.3% identity in 540 aa overlap); pknE (566 aa); pknB (626 aa); Rv3524 (343 aa); etc. Contains Hank's kinase subdomain. Contains two transmembrane segments, which flank a highly repetitive region, suggesting a receptor-like anchoring. Belongs to the Ser/Thr family of protein kinases. Experimental studies show evidence of auto-phosphorylation on a serine residue. Appears to be co-transcribed with Rv0932c|pstS2. O05871 No regulatory proteins ML0743 MMAR_4577 Mb0954c,Mb0955c NC_000962.3 Mycobrowser_v2 CDS 1039936 1041048 . - 0 Rv0932c pstS2 Involved in active transport of inorganic phosphate across the membrane (import). This is one of the proteins required for binding-protein-mediated phosphate transport. Periplasmic phosphate-binding lipoprotein PstS2 (PBP-2) (PstS2) Rv0932c, (MTCY08D9.07), len: 370 aa. PstS2, phosphate-binding lipoprotein component of inorganic phosphate transport system (see citations below), highly similar to AAF74819.1|AF137360_1|AF137360 periplasmic phosphate permease from Mycobacterium avium (369 aa); Rv0928|MTCY21C12.22|pstS3 phosphate-binding periplasmic lipoprotein from Mycobacterium tuberculosis (370 aa), FASTA scores: opt: 1601, E(): 0, (64.5% identity in 372 aa overlap); and Rv0934|MTCY08D9.05c|pstS1 phosphate-binding periplasmic lipoprotein from Mycobacterium tuberculosis (374 aa) (Mycobacterium tuberculosis seems to have three PstS-like proteins, others being Rv0928 and Rv0934c). Also highly similar to MTCY08D9.05c|P15712|PAB_MYCTU protein antigen B precursor from Mycobacterium tuberculosis (374 aa), FASTA scores: opt: 460, E(): 2.7e-20, (31.2% identity in 375 aa overlap). Contains prokaryotic membrane lipoprotein lipid attachment site (PS00013) at N-terminus so the leader peptide of 22 aa is probably removed. Belongs to family of phosphate receptors for bacterial ABC-type lipoprotein transporters. Appears to be co-transcribed with Rv0931c|pknD|mbk. O05870 No cell wall and cell processes ML0742 MMAR_4576 Mb0956c NC_000962.3 Mycobrowser_v2 RBS 1041054 1041058 . - 0 Rv0932c pstS2 Involved in active transport of inorganic phosphate across the membrane (import). This is one of the proteins required for binding-protein-mediated phosphate transport. Periplasmic phosphate-binding lipoprotein PstS2 (PBP-2) (PstS2) Rv0932c, (MTCY08D9.07), len: 370 aa. PstS2, phosphate-binding lipoprotein component of inorganic phosphate transport system (see citations below), highly similar to AAF74819.1|AF137360_1|AF137360 periplasmic phosphate permease from Mycobacterium avium (369 aa); Rv0928|MTCY21C12.22|pstS3 phosphate-binding periplasmic lipoprotein from Mycobacterium tuberculosis (370 aa), FASTA scores: opt: 1601, E(): 0, (64.5% identity in 372 aa overlap); and Rv0934|MTCY08D9.05c|pstS1 phosphate-binding periplasmic lipoprotein from Mycobacterium tuberculosis (374 aa) (Mycobacterium tuberculosis seems to have three PstS-like proteins, others being Rv0928 and Rv0934c). Also highly similar to MTCY08D9.05c|P15712|PAB_MYCTU protein antigen B precursor from Mycobacterium tuberculosis (374 aa), FASTA scores: opt: 460, E(): 2.7e-20, (31.2% identity in 375 aa overlap). Contains prokaryotic membrane lipoprotein lipid attachment site (PS00013) at N-terminus so the leader peptide of 22 aa is probably removed. Belongs to family of phosphate receptors for bacterial ABC-type lipoprotein transporters. Appears to be co-transcribed with Rv0931c|pknD|mbk. O05870 No cell wall and cell processes ML0742 MMAR_4576 Mb0956c NC_000962.3 Mycobrowser_v2 CDS 1041264 1042094 . + 0 Rv0933 pstB Involved in active transport of inorganic phosphate across the membrane (import); responsible for energy coupling to the transport system. This is one of the proteins required for binding-protein-mediated phosphate transport. Have ATP-binding ability and ATPase activity. Phosphate-transport ATP-binding protein ABC transporter PstB Rv0933, (MTCY08D9.06c), len: 276 aa. PstB, phosphate-transport ATP-binding protein ABC transporter (see citations below), thermostable ATPase, highly similar to others e.g. NP_348334.1|NC_003030 ATPase component of ABC-type phosphate transport system from Clostridium acetobutylicum (249 aa); NP_212352.1|NC_001318 phosphate ABC transporter ATP-binding protein (pstB) from Borrelia burgdorferi (260 aa); NP_390375.1|NC_000964 phosphate ABC transporter (ATP-binding protein) from Bacillus subtilis (269 aa), FASTA scores: opt: 762, E(): 0, (47.7% identity in 243 aa overlap); etc. Also similar to other M. tuberculosis ABC transporters e.g. MTCY253.24, E(): 2.5e-15 and MTCY359.14c, E(): 3.4e-15. Contains PS00211 ABC transporters family signature, and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters). Magnesium or calcium seem to have no influence on the functionality of this enzyme. P95302 No cell wall and cell processes ML0741 Mb0957,Mb0958 NC_000962.3 Mycobrowser_v2 CDS 1053765 1054241 . + 0 Rv0944 Rv0944 This enzyme may play a significant role in processes leading to recovery from mutagenesis and/or cell death by alkylating agents [catalytic activity: hydrolysis of DNA containing ring-opened N7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimide]. Possible formamidopyrimidine-DNA glycosylase (FAPY-DNA glycosylase) Rv0944, (MTCY10D7.30c), len: 158 aa. Possible formamidopyrimidine-DNA glycosylase, similar to C-terminus of formamidopyrimidine-DNA glycosylases e.g. CAB63194.1|AL133469 putative formamidopyrimidine-DNA glycosylase from Streptomyces coelicolor (287 aa); FPG_LACLA|NP_266509.1|NC_002662 formamidopyrimidine-DNA glycosylase from Lactococcus lactis subsp. lactis (273 aa), FASTA scores: opt: 246, E(): 2.4e-09, (28.9% identity in 142 aa overlap); O50606|FPG_THETH|MUTM|FPG formamidopyrimidine-DNA glycosylase from Thermus thermophilus (267 aa); etc. Also similar to C-termini of endonucleases or DNA glycosylases of Mycobacterium tuberculosis e.g. Rv3297, Rv2464c, Rv2924c. May belong to the FPG family. P71565 No information pathways ML0148 Mb0969 NC_000962.3 Mycobrowser_v2 CDS 1042115 1043239 . + 0 Rv0934 pstS1 Involved in active transport of inorganic phosphate across the membrane (import). This is one of the proteins required for binding-protein-mediated phosphate transport. Periplasmic phosphate-binding lipoprotein PstS1 (PBP-1) (PstS1) Rv0934, (MTCY08D9.05c), len: 374 aa. PstS1 (previously known as phoS1 or phoS), phosphate-binding lipoprotein component of inorganic phosphate transport system (see citations below), highly similar to Rv0932c|MTCY08D9.07|pstS2 phosphate-binding periplasmic lipoprotein from Mycobacterium tuberculosis (370 aa), FASTA scores: opt: 460, E(): 5.9e-19, (31.2% identity in 375 aa overlap); and Rv0928|MTCY21C12.22|pstS3 phosphate-binding periplasmic lipoprotein from Mycobacterium tuberculosis (374 aa), FASTA scores: opt: 435, E():1.1e-17, (30.0% identity in 380 aa overlap) (Mycobacterium tuberculosis seems to have three PstS-like proteins, others being Rv0932c and Rv0928c). Also highly similar to MTCY08D9.05c|P15712|PAB_MYCTU protein antigen B precursor from Mycobacterium tuberculosis (374 aa), FASTA scores: opt: 2459, E(): 0, (100% identity in 374 aa overlap). Contains a prokaryotic membrane lipoprotein lipid attachment site (PS00013) at the N-terminus so the 23 aa leader peptide sequence is probably removed. Belongs to family of phosphate receptors for bacterial ABC-type lipoprotein transporters. P15712 No cell wall and cell processes ML0740 Mb0959 NC_000962.3 Mycobrowser_v2 CDS 1043299 1044315 . + 0 Rv0935 pstC1 Involved in active transport of inorganic phosphate across the membrane (import); responsible for the translocation of the substrate across the membrane. This is one of the proteins required for binding-protein-mediated phosphate transport. Phosphate-transport integral membrane ABC transporter PstC1 Rv0935, (MTCY08D9.04c), len: 338 aa. PstC1, phosphate-transport integral membrane ABC transporter (see citations below), highly similar to others e.g. NP_104768.1|NC_002678|pstC phosphate ABC transporter permease protein from Mesorhizobium loti (327 aa); NP_245372.1|NC_002663|PstC PstC protein from Pasteurella multocida (320 aa); P45191|PSTC_HAEIN phosphate transport system permease from Haemophilus influenza (315 aa), FASTA scores: opt: 667, E(): 0, (36.2% identity in 309 aa overlap); etc. Also similar to Rv0929|MTCY21C12.23|PSTC2 probable transmembrane ABC transporter component of phosphate uptake system from Mycobacterium tuberculosis (324 aa), FASTA scores: opt: 487, E(): 4.1e-21, (32.3% identity in 303 aa overlap); and shows slight similarity to MTCY08D9.03c|PSTA2|Rv0936 probable transmembrane ABC transporter component of phosphate uptake system from Mycobacterium tuberculosis (301 aa). Contains binding-protein-dependent transport systems inner membrane comp signature (PS00402). P0A628 No cell wall and cell processes ML0739 Mb0960 NC_000962.3 Mycobrowser_v2 CDS 1044317 1045222 . + 0 Rv0936 pstA2 Involved in active transport of inorganic phosphate across the membrane (import); responsible for the translocation of the substrate across the membrane. This is one of the proteins required for binding-protein-mediated phosphate transport. Phosphate-transport integral membrane ABC transporter PstA2 Rv0936, (MTCY08D9.03c), len: 301 aa. PstA2, phosphate-transport integral membrane ABC transporter (see citations below), highly similar to others e.g. NP_442269.1|NC_000911|PstA phosphate transport system permease protein from Synechocystis sp. strain PCC 6803 (287 aa); NP_232473.1|NC_002506 phosphate ABC transporter permease protein from Vibrio cholerae (289 aa); P07654|PSTA_ECOLI phosphate transport system permease from Escherichia coli (296 aa), FASTA scores: opt: 464, E(): 6.7e-24, (30.5% identity in 282 aa overlap); etc. Also similar to O86345|MTCY21C12.24|PSTA1|Rv0930 probable transmembrane ABC transporter component of phosphate uptake system from Mycobacterium tuberculosis (304 aa), FASTA scores: opt: 369, E(): 6.1e-15, (32.7% identity in 248 aa overlap). Contains binding-protein-dependent transport systems inner membrane comp signature (PS00402). P0A626 No cell wall and cell processes ML0738 Mb0961 NC_000962.3 Mycobrowser_v2 CDS 1050593 1051459 . - 0 Rv0940c Rv0940c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0940c, (MTCY10D7.34), len: 288 aa. Possible oxidoreductase, similar to hypothetical proteins and oxidoreductases e.g. AAK38097.1|AF323606_3|AF323606 putative F420-dependent dehydrogenase from Rhodococcus erythropolis (295 aa); AAG52987.1|AF040570|Rif17 putative alkanal monooxygenase from Amycolatopsis mediterranei (356 aa); etc. Also similar to putative oxidoreductases from Mycobacterium tuberculosis such as Rv0953c|P71557|YT21_MYCTU (282 aa), FASTA scores: opt: 311, E(): 3.7e-08, (31.0% identity in 248 aa overlap), Rv3079c (275 aa), Rv0791c (347 aa), etc. P64761 No intermediary metabolism and respiration MMAR_4568 Mb0965c NC_000962.3 Mycobrowser_v2 CDS 1045199 1046020 . - 0 Rv0937c mku Involved in DNA double-strand break repair, by nonhomologous end joining (NHEJ). Interacts with LIGD (Rv0938). DNA end-binding protein, Mku Rv0937c, (MTCY08D9.02), len: 273 aa. Mku, DNA end-binding protein, highly similar to others e.g. SC6G9.24c|T35620|AL079356 hypothetical protein from Streptomyces coelicolor (365 aa), FASTA scores: opt: 648, E(): 0, (36.5% identity in 274 aa overlap); Z99110|BSUB0007_223|NP_389224.1|NC_000964 hypothetical proteins from Bacillus subtilis (311 aa), FASTA scores: opt: 623, E(): 1.1e-31, (33.9% identity in 274 aa overlap); O28548|AE000984|AF1726|NP_070554.1|NC_000917 conserved hypothetical protein from Archaeoglobus fulgidus (286 aa), FASTA scores: opt: 583, E(): 0, (36.6% identity in 262 aa overlap); etc. O05866 No information pathways ML2092 MMAR_4575 MSMEG_5580 Mb0962c NC_000962.3 Mycobrowser_v2 CDS 1054247 1055008 . + 0 Rv0945 Rv0945 Function unknown; possibly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv0945, (MTCY10D7.29c), len: 253 aa. Probable short-chain dehydrogenase/reductase, similar to various dehydrogenases/reductases e.g. NP_346338.1|NC_003028 oxidoreductase (short chain dehydrogenase/reductase family) from Streptococcus pneumoniae (253 aa); AAB70845.1|AF019986|PksB from Dictyostelium discoideum (260 aa); AAF86624.1|U87786 clavaldehyde dehydrogenase from Streptomyces clavuligerus (247 aa); P37440|UCPA_ECOLI oxidoreductase from Escherichia coli (285 aa), FASTA scores: opt: 275, E(): 1.1e-12, (33.8% identity in 201 aa overlap); etc. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. P71564 No intermediary metabolism and respiration ML0149 MMAR_4558 MSMEG_5544 Mb0970 NC_000962.3 Mycobrowser_v2 CDS 1046136 1048415 . + 0 Rv0938 ligD Involved in DNA double-strand break repair, by nonhomologous end joining (NHEJ). Interacts with KU (Rv0937c) [catalytic activity:ATP + {deoxyribonucleotide}(N) + {deoxyribonucleotide}(M) = AMP + diphosphate + {deoxyribonucleotide}(N+M)]. ATP dependent DNA ligase LigD (ATP dependent polydeoxyribonucleotide synthase) (thermostable DNA ligase) (ATP dependent polynucleotide ligase) (sealase) (DNA repair enzyme) (DNA joinase) Rv0938, (MTCY08D9.01c, MTCY10D7.36c), len: 759 aa. ligD, ATP-dependent DNA ligase, with its C-terminus similar to N-terminal parts of many ATP-dependent DNA ligases e.g. NP_250828.1|NC_002516 probable ATP-dependent DNA ligase from Pseudomonas aeruginosa (840 aa); NP_105436.1|NC_002678 ATP-dependent DNA ligase from Mesorhizobium loti (829 aa); CAB92891.1|AL356932 probable ATP-dependent DNA ligase from Streptomyces coelicolor (326 aa); etc. The N-terminal half shows similarity with hypothetical proteins from Mycobacterium tuberculosis Rv0269c and Rv3730c; and the C-terminal half with the DNA ligases Rv3731 and Rv3062. P71571 No information pathways ML2090 MMAR_4573 MSMEG_5570 Mb0963 NC_000962.3 Mycobrowser_v2 CDS 1048412 1050346 . + 0 Rv0939 Rv0939 Function unknown; probably involved in cellular metabolism, possibly in a degradation pathway. Possible bifunctional enzyme: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (HHDD isomerase) + cyclase/dehydrase Rv0939, (MTCY10D7.35c), len: 644 aa. Possible bifunctional enzyme, including 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase activity, and cyclase/dehydrase activity. N-terminal part similar to many isomerases e.g. NP_343861.1|NC_002754 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (hpcE-1) from Sulfolobus solfataricus (318 aa); NP_068932.1|NC_000917 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (hpcE-1) from Archaeoglobus fulgidus (324 aa), FASTA scores: opt: 400, E(): 5.8e-15, (33.9% identity in 289 aa overlap); etc. And C-terminal part highly similar to many cyclases/dehydrases e.g. AAK61721.1|AY033994 cyclase-like protein from Streptomyces aureofaciens (305 aa); CAC44204.1|AL593842 cyclase from Streptomyces coelicolor (297 aa), FASTA scores: opt: 375, E(): 2.7e-26, (35.6% identity in 284 aa overlap); NP_343860.1|NC_002754 putative Cyclase/dehydrase from Sulfolobus solfataricus (308 aa); etc. Also similar to Rv2993c hypothetical protein from Mycobacterium tuberculosis. O86346 No intermediary metabolism and respiration ML2089 MMAR_4572 Mb0964 NC_000962.3 Mycobrowser_v2 CDS 1052696 1053736 . - 0 Rv0943c Rv0943c Function unknown; probably involved in cellular metabolism. Probable monooxygenase Rv0943c, (MTCY10D7.31), len: 346 aa. Possible monooxygenase, similar in part to others e.g. NP_250229.1|NC_002516 probable flavin-containing monooxygenase from Pseudomonas aeruginosa (527 aa); AAC36351.1|AF090329 cyclohexanone monooxygenase homolog from Pseudomonas fluorescens (437 aa); CAB59668.1|AL132674 monooxygenase from Streptomyces coelicolor (519 aa); etc. Also similar to putative monooxygenases from Mycobacterium tuberculosis e.g. Rv1393c|P71662|CY21B4.10C (492 aa). FASTA scores: opt: 129, E(): 8.5e-21, (27.5% identity in 236 aa overlap); Rv0892 (495 aa); Rv3049c (524 aa); etc. P64765 No intermediary metabolism and respiration MMAR_4565 Mb0968c NC_000962.3 Mycobrowser_v2 CDS 1077975 1078334 . + 0 Rv0967 csoR Involved in transcriptional mechanism; repression of the CSO operon. Copper-sensitive operon repressor CsoR Rv0967, (MTCY10D7.07c), len: 119 aa. CsoR, copper-sensitive operon repressor, part of cso operon (See Liu et al., 2007), similar to hypothetical proteins from several organisms e.g. AE002074|AE002074_11 from Deinococcus radiodurans (102 aa), FASTA scores: opt: 233, E(): 8.6e-10, (47.0% identity in 83 aa overlap); O32222|Z99121|YVGZ from Bacillus subtilis (101 aa), FASTA scores: opt:228, E(): 3.2e-15, (38.0% identity in 92 aa overlap); etc. Also similar to Mycobacterium tuberculosis hypothetical proteins Rv0190, and Rv1766. P71543 No regulatory proteins MMAR_4874 Mb0992 NC_000962.3 Mycobrowser_v2 CDS 1055024 1056685 . - 0 Rv0946c pgi Involved in glycolysis and in gluconeogenesis [catalytic activity: D-glucose 6-phosphate = D-fructose 6-phosphate]. Probable glucose-6-phosphate isomerase Pgi (GPI) (phosphoglucose isomerase) (phosphohexose isomerase) (phi) Rv0946c, (MTCY10D7.28), len: 553 aa. Probable pgi, glucose-6-phosphate isomerase, equivalent to NP_301236.1|NC_002677 glucose-6-phosphate isomerase from Mycobacterium leprae (554 aa); and P96803|G6PI_MYCSM glucose-6-phosphate isomerase from Mycobacterium smegmatis (442 aa). Also highly similar to others e.g. T36015 glucose-6-phosphate isomerase from Streptomyces coelicolor (551 aa); P11537|G6PI_ECOLI|GPI glucose-6-phosphate isomerase from Escherichia coli strains K12 and O157:H7 (549 aa), FASTA scores: opt: 1779, E(): 0, (51.4% identity in 554 aa overlap); etc. Contains PS00765 Phosphoglucose isomerase signature 1, and PS00174 Phosphoglucose isomerase signature 2. Belongs to the GPI family. P64192 No intermediary metabolism and respiration ML0150 MMAR_4557 MSMEG_5541 Mb0971c NC_000962.3 Mycobrowser_v2 CDS 1057300 1057530 . - 0 Rv0947c Rv0947c Function unknown; thought to be involved in mycolic acid biosynthesis. Probable mycolyl transferase, pseudogene Rv0947c, (MTCY10D7.27), len: 76 aa. Probable mycolyl transferase pseudogene, similar to part of P31953|A85C_MYCTU|fbpC2 antigen 85-c precursor (85c) (fibronectin-binding protein C) from Mycobacterium tuberculosis (340 aa), FASTA scores: opt: 213, E(): 2e-08, (69.6% identity in 46 aa overlap). Yes lipid metabolism Mb0972c NC_000962.3 Mycobrowser_v2 CDS 1977328 1977885 . - 0 Rv1749c Rv1749c Unknown Possible integral membrane protein Rv1749c, (MTCY28.12c-MTCY04C12.33c), len: 185 aa. Possible integral membrane protein, similar to O27914|AE000940 hypothetical protein MTH1892 from Methanobacterium thermoautotrophicum (168 aa), fasta scores: E(): 9.3e-16, (37.4% identity in 123 aa overlap). O65935 No cell wall and cell processes MMAR_2101 Mb1778c NC_000962.3 Mycobrowser_v2 CDS 1057646 1057963 . - 0 Rv0948c Rv0948c Involved in the shikimate pathway. Converts chorismate to prephenate in the biosynthesis of tyrosine and phenylalanine. Chorismate mutase Rv0948c, (MTCY10D7.26), len: 105 aa. Chorismate mutase, AroQ class (See Prakash et al., 2005; Schneider et al., 2008), equivalent to NP_301237.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (105 aa). Also similar (except in N-terminus) to SCD63.16c|CAB82023.1|AL161755 hypothetical protein from Streptomyces coelicolor (110 aa); and to N-terminus of two chorismate mutase/prephenate dehydratase. P64767 No intermediary metabolism and respiration ML0151 MMAR_4555 MSMEG_5536 Mb0973c NC_000962.3 Mycobrowser_v2 CDS 1058260 1060575 . + 0 Rv0949 uvrD1 Involved in nucleotide excision repair. Has a 3'-5' helicase activity in presence of ATP. Preferred substrate being one with both single and double stranded regions of DNA. Probable ATP-dependent DNA helicase II UvrD1 Rv0949, (MTCY10D7.25c), len: 771 aa. Probable uvrD1, ATP dependent DNA helicase II (see citation below), equivalent to P_301239.1|NC_002677 putative ATP-dependent DNA helicase from Mycobacterium leprae (778 aa). Also highly similar to others e.g. CAB92660.1|AL356832 from Streptomyces coelicolor (831 aa) (N-terminus longer); P56255|PCRA_BACST from Bacillus stearothermophilus (724 aa); Q10213|YAY5_SCHPO from Schizosaccharomyces pombe (Fission yeast) (887 aa), FASTA scores: opt: 927, E(): 0, (33.5% identity in 659 aa overlap); etc. Also similar to several other UvrD-like proteins in Mycobacterium tuberculosis e.g. Rv3201c, Rv3198c, Rv3202c. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the UVRD subfamily of helicases. Note that previously known as uvrD. P0A5A3 No information pathways ML0153 MMAR_4553 MSMEG_5534 Mb0974 NC_000962.3 Mycobrowser_v2 CDS 1060656 1061654 . - 0 Rv0950c Rv0950c Function unknown Conserved hypothetical protein Rv0950c, (MTCY10D7.24), len: 332 aa. Conserved hypothetical protein, highly similar to AL035500|MLCL373.02c|T45433 hypothetical protein from Mycobacterium leprae (343 aa), FASTA scores: opt: 1500, E(): 0, (71.0% identity in 331 aa overlap). C-terminus highly similar to part of various proteins e.g. C-terminal part of NP_441943.1|NC_000911|NlpD lipoprotein from Synechocystis sp (715 aa); N-terminal part of NP_066789.1|NC_002576 putative peptidase from Rhodococcus equi (546 aa); C-terminal part of NP_212396.1|NC_001318 conserved hypothetical protein from Borrelia burgdorferi (417 aa); C-terminal part of P33648|NLPD_ECOLI|nlpd lipoprotein from Escherichia coli (379 aa), FASTA scores: opt: 276, E(): 2e-10, (29.9% identity in 234 aa overlap); etc. P71560 No conserved hypotheticals ML0154 MMAR_4551 MSMEG_5526 Mb0975c NC_000962.3 Mycobrowser_v2 CDS 1061964 1063127 . + 0 Rv0951 sucC Involved in tricarboxylic acid cycle [catalytic activity: ATP + succinate + CoA = ADP + succinyl-CoA + phosphate]. Probable succinyl-CoA synthetase (beta chain) SucC (SCS-beta) Rv0951, (MTCY10D7.23c), len: 387 aa. Probable sucC, succinyl-CoA synthetase, beta chain, equivalent to AL035500|MLCL373_3|NP_301241.1|NC_002677 succinyl-CoA synthase [beta] chain from Mycobacterium leprae (393 aa), FASTA score: (86.7% identity in 391 aa overlap). Also highly similar to others e.g. AB92671.1|AL356832 succinyl-CoA synthetase beta chain from Streptomyces coelicolor (394 aa); P25126|SUCC_THEFL succinyl-CoA synthetase beta chain from Thermus aquaticus (378 aa); P07460|SUCC_ECOLI succinyl-CoA synthetase beta chain from Escherichia coli (388 aa), FASTA scores: opt: 933, E(): 0, (41.0% identity in 390 aa overlap); etc. P71559 No intermediary metabolism and respiration ML0155 MMAR_4550 MSMEG_5525 Mb0976 NC_000962.3 Mycobrowser_v2 CDS 3136330 3136599 . - 0 Rv2828A Rv2828A Unknown Conserved hypothetical protein Rv2828A, len: 89 aa. Conserved hypothetical protein, present in many mycobacteria. Equivalent to BCG2848c and Mb2852A (100% identity to both in 89 aa overlap) No conserved hypotheticals NC_000962.3 Mycobrowser_v2 CDS 1063140 1064051 . + 0 Rv0952 sucD Involved in tricarboxylic acid cycle [catalytic activity: ATP + succinate + CoA = ADP + succinyl-CoA + phosphate]. Probable succinyl-CoA synthetase (alpha chain) SucD (SCS-alpha) Rv0952, (MTCY10D7.22c), len: 303 aa. Probable sucD, succinyl-CoA synthetase, alpha chain, equivalent to AL035500|MLCL373_4|NP_301242.1|NC_002677 succinyl-CoA synthase [alpha] chain from Mycobacterium leprae (300 aa), FASTA score: (86.3% identity in 300 aa overlap). Also highly similar to others e.g. CAB92672.1|AL356832 from Streptomyces coelicolor (294 aa); P53591|SUCD_COXBU from Escherichia coli (288 aa), FASTA scores: opt: 855, E(): 0, (53.8% identity in 286 aa overlap); etc. Contains PS00399 ATP-citrate lyase and succinyl-CoA ligases active site, and PS00017 ATP/GTP-binding site motif A (P-loop). P71558 No intermediary metabolism and respiration ML0156 MMAR_4549 MSMEG_5524 Mb0977 NC_000962.3 Mycobrowser_v2 CDS 1065127 1066038 . + 0 Rv0954 Rv0954 Unknown Probable conserved transmembrane protein Rv0954, (MTCY10D7.20c), len: 303 aa. Probable conserved transmembrane protein, highly similar to 34KD_MYCPA|Q04959 34 kDa antigenic protein from Mycobacterium paratuberculosis (298 aa), FASTA scores: opt: 1023, E(): 7.2e-36, (59.3% identity in 305 aa overlap); AAC69251.1|U82111 34 kDa antigen precursor from Mycobacterium leprae (336 aa); and AL035500|MLCL373.06 hypothetical membrane protein from Mycobacterium leprae (297 aa), FASTA score: (55.6% identity in 315 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P65637 No cell wall and cell processes ML0158 MMAR_4545 MSMEG_5518 Mb0979 NC_000962.3 Mycobrowser_v2 CDS 1066078 1067445 . + 0 Rv0955 Rv0955 Unknown Probable conserved integral membrane protein Rv0955, (MTCY10D7.19c), len: 455 aa. Probable conserved integral membrane protein, highly similar to AL035500|MLCL373_6 putative membrane protein from Mycobacterium leprae (430 aa), FASTA score: (75.9% identity in 419 aa overlap); and AAL05878.1|AF411607_2|AF411607 unknown protein from Mycobacterium avium subsp. paratuberculosis (409 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P64771 No cell wall and cell processes ML0159 MMAR_4544 MSMEG_5517 Mb0980 NC_000962.3 Mycobrowser_v2 CDS 1067561 1068208 . + 0 Rv0956 purN Involved in de novo purine biosynthesis (at the third step) [catalytic activity: 10-formyltetrahydrofolate + 5'-phosphoribosylglycinamide = tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide]. Probable 5'-phosphoribosylglycinamide formyltransferase PurN (GART) (gar transformylase) (5'-phosphoribosylglycinamide transformylase) Rv0956, (MTCY10D7.18c), len: 215 aa. Probable purN, 5'-phosphoribosylglycinamide formyltransferase, equivalent to AAF05726.1|AF191543_1|AF191543|PurN phosphoribosylglycinamide formyltransferase from Mycobacterium avium subsp. paratuberculosis (209 aa); and AL035500|MLCL373_7 from Mycobacterium leprae (215 aa), FASTA score: (79.4% identity in 214 aa overlap). Also highly similar to others e.g. BAA89443.1|AB003159 from Corynebacterium ammoniagenes (199 aa); NP_241498.1|NC_002570 from Bacillus halodurans (188 aa); P08179|PUR3_ECOLI|B2500 from Escherichia coli strain K12 (212 aa), FASTA scores: opt: 380, E(): 2.4e-18, (36.6% identity in 183 aa overlap); C-terminus of P16340|PUR2_DROPS trifunctional purine biosynthetic protein adenosine-3 from Drosophila pseudoobscura (Fruit fly) (1364 aa); etc. P71554 No intermediary metabolism and respiration ML0160 MMAR_4543 MSMEG_5516 Mb0981 NC_000962.3 Mycobrowser_v2 CDS 1068205 1069776 . + 0 Rv0957 purH Involved in de novo purine biosynthesis (at the ninth and tenth steps) [catalytic activity 1: 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-4-imidazolecarboxamide = tetrahydrofolate + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide] [catalytic activity 2: imp + H2O = 5-formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide]. Probable bifunctional purine biosynthesis protein PurH: phosphoribosylaminoimidazolecarboxamide formyltransferase (AICAR transformylase) (5'-phosphoribosyl-5-aminoimidazole-4-carboxamide formyltransferase) + inosinemonophosphate cyclohydrolase (imp cyclohydrolase) (inosinicase) (imp synthetase) (ATIC) Rv0957, (MTCY10D7.17c), len: 523 aa. Probable purH, bifunctional purine biosynthesis protein including 5'-phosphoribosyl-5-aminoimidazole-4-carboxamide formyltransferase and inosine-monophosphate (imp) cyclohydrolase, equivalent to AL035500|MLCL373_8 putative phosphoribosylaminoimidazolecarboxamide formyltransferase from Mycobacterium leprae (527 aa), FASTA score: (88.1% identity in 520 aa overlap); and AF05727.1|AF191543_2|AF191543|PurH from Mycobacterium avium subsp. paratuberculosis (527 aa). Also highly similar to others e.g. CAB92677.1|AL356832 bifunctional purine biosynthesis protein from Streptomyces coelicolor (523 aa); NP_388534.1|NC_000964 phosphoribosylaminoimidazole carboxy formyl formyltransferase + inosine-monophosphate cyclohydrolase from Bacillus subtilis (512 aa); P15639|PUR9_ECOLI phosphoribosylaminoimidazolecarboxamide formyltransferase from Escherichia coli (529 aa), FASTA scores: opt: 1147, E(): 0, (44.8% identity in 533 aa overlap); etc. Belongs to the PurH family. P67541 No intermediary metabolism and respiration ML0161 MMAR_4542 MSMEG_5515 Mb0982 NC_000962.3 Mycobrowser_v2 CDS 1076778 1077197 . - 0 Rv0965c Rv0965c Function unknown Conserved hypothetical protein Rv0965c, (MTCY10D7.09), len: 139 aa. Conserved hypothetical protein, showing weak similarity with Rv2798c|MTCY16B7.45 conserved hypothetical protein from Mycobacterium tuberculosis (108 aa), FASTA scores: E(): 5.6e-12, (38.9% identity in 90 aa overlap). Equivalent to AAK45242.1 from Mycobacterium tuberculosis strain CDC1551 (146 aa) but shorter 7 aa. P71545 No conserved hypotheticals ML0170 Mb0990c NC_000962.3 Mycobrowser_v2 CDS 1069883 1071262 . + 0 Rv0958 Rv0958 Chelation, introducing a magnesium ion into specific substrate. Possible magnesium chelatase Rv0958, (MTCY10D7.16c), len: 459 aa. Possible magnesium chelatase, similar to others (especially in N-terminal parts) e.g. NP_296313.1|NC_001263|AE002088_10 putative magnesium protoporphyrin chelatase from Deinococcus radiodurans (487 aa), FASTA scores: opt: 1148, E(): 0, (42.4% identity in 450 aa overlap); Q44498|CHLI_ANAVA magnesium-chelatase subunit CHLI from Anabaena variabilis (338 aa); T31460 probable magnesium chelatase chain I bchI from Heliobacillus mobilis (363 aa); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). P71552 No intermediary metabolism and respiration MMAR_4541 MSMEG_5512 Mb0983 NC_000962.3 Mycobrowser_v2 CDS 1071255 1073273 . + 0 Rv0959 Rv0959 Function unknown Conserved hypothetical protein Rv0959, (MTCY10D7.15c), len: 672 aa. Conserved hypothetical protein, similar to AE002069|AE002069_12 hypothetical protein from Deinococcus radiodurans (403 aa), FASTA scores: opt: 395, E(): 1.3e-15, (26.8% identity in 426 aa overlap). Contains a single copy at the N-terminus of a short repeat found three times in the M. tuberculosis ORF O33341|MTV003.05c|AL008883. P0A5D7 No conserved hypotheticals ML0163 MMAR_4540 MSMEG_5511 Mb0984 NC_000962.3 Mycobrowser_v2 CDS 1073327 1073548 . + 0 Rv0959A vapB9 Unknown Possible antitoxin VapB9 Rv0959A, len: 73 aa. Possible vapB9, antitoxin, part of toxin-antitoxin (TA) operon with Rv0960 (See Arcus et al., 2005; Pandey and Gerdes, 2005). Weakly similar to others in Mycobacterium tuberculosis e.g. Rv1721c P0CW30 No virulence, detoxification, adaptation NC_000962.3 Mycobrowser_v2 CDS 1073545 1073928 . + 0 Rv0960 vapC9 Unknown Possible toxin VapC9 Rv0960, (MTCY10D7.14c), len: 127 aa. Possible vapC9, toxin, part of toxin-antitoxin (TA) operon with Rv0959A, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. Rv0065|MTV030.08 (133 aa), FASTA scores: E(): 1.5e-14, (38.3% identity in 128 aa overlap), Rv1720c (129 aa), and Rv0549c (137 aa). P64773 No virulence, detoxification, adaptation Mb0985 NC_000962.3 Mycobrowser_v2 CDS 1074074 1074421 . + 0 Rv0961 Rv0961 Unknown Probable integral membrane protein Rv0961, (MTCY10D7.13c), len: 115 aa. Probable integral membrane protein. P64775 No cell wall and cell processes Mb0986 NC_000962.3 Mycobrowser_v2 CDS 1074440 1075114 . - 0 Rv0962c lprP Unknown Possible lipoprotein LprP Rv0962c, (MTCY10D7.12), len: 224 aa. Possible lprP, lipoprotein. Contains possible N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. P71548 No cell wall and cell processes ML0172 Mb0987c NC_000962.3 Mycobrowser_v2 CDS 1075297 1076097 . - 0 Rv0963c Rv0963c Function unknown Conserved hypothetical protein Rv0963c, (MTCCY10D7.11), len: 266 aa. Conserved hypothetical protein, similar in part to other conserved hypothetical proteins from Mycobacterium tuberculosis e.g. Rv2797c|MTCY16B7.46 (562 aa), FASTA scores: E(): 1.2e-23, (39.0% identity in 254 aa overlap); Rv2542 (403 aa); Rv2079 (656 aa). Also similar in part to AL133423|SC4A7_3 hypothetical secreted protein from Streptomyces coelicolor (406 aa), FASTA scores: opt: 231, E(): 6.8e-07, (31.4% identity in 204 aa overlap); and SCH10.21c|T36533 hypothetical protein from Streptomyces coelicolor (329 aa). P64777 No conserved hypotheticals ML0171 Mb0988c NC_000962.3 Mycobrowser_v2 CDS 1077233 1077835 . - 0 Rv0966c Rv0966c Function unknown Conserved protein Rv0966c, (MTCY10D7.08), len: 200 aa. Conserved protein, equivalent to AL035500|MLCL373_12 conserved hypothetical protein from Mycobacterium leprae (200 aa), FASTA scores: opt: 1080, E(): 0, (79.5% identity in 200 aa overlap). Also highly similar to SCE6.30c|CAB88834.1|AL353832 hypothetical protein from Streptomyces coelicolor (277 aa). Some similarity to Rv2862c|MTV007.08 conserved hypothetical protein from Mycobacterium tuberculosis (194 aa), FASTA scores: E(): 3.1e-06, (31.5% identity in 184 aa overlap). Equivalent to AAK45243.1 from Mycobacterium tuberculosis strain CDC1551 (230 aa) but shorter 30 aa. Note that Rv0966c has been shortened since first entry. P71544 No conserved hypotheticals ML0169 MMAR_4539 MSMEG_5505 Mb0991c NC_000962.3 Mycobrowser_v2 CDS 1078743 1081055 . + 0 Rv0969 ctpV Metal cation-transporting ATPase; possibly catalyzes the transport of an undetermined metal cation with the hydrolysis of ATP [catalytic activity: ATP + H(2)O + undetermined metal cation(in) = ADP + phosphate + undetermined metal cation(out)]. Probable metal cation transporter P-type ATPase CtpV Rv0969, (MTCY10D7.05c), len: 770 aa. Probable ctpV, metal cation transporter P-type ATPase (transmembrane protein) (see citation below), part of cso operon, highly similar (except in N-terminus) to others e.g. NP_391230.1|NC_000964 similar to heavy metal-transporting ATPase from Bacillus subtilis (803 aa); P37279|ATCS_SYNP7|PACS cation-transporting ATPase from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (747 aa), FASTA scores: opt: 1851, E(): 0, (52.1% identity in 664 aa overlap); etc. Equivalent to AAK45246.1 from Mycobacterium tuberculosis strain CDC1551 (792 aa) but shorter 22 aa. Contains PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases). P77894 No cell wall and cell processes MMAR_1431 MSMEG_5014 Mb0994 NC_000962.3 Mycobrowser_v2 CDS 1081052 1081684 . + 0 Rv0970 Rv0970 Unknown Probable conserved integral membrane protein Rv0970, (MTCY10D7.04c), len: 210 aa. Probable conserved integral membrane protein, part of cso operon, equivalent to NP_302348.1|NC_002677 probable integral membrane protein from Mycobacterium leprae (210 aa). P64781 No cell wall and cell processes ML1997 MMAR_1432,MMAR_4877 Mb0995 NC_000962.3 Mycobrowser_v2 CDS 1081775 1082584 . - 0 Rv0971c echA7 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Probable enoyl-CoA hydratase EchA7 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv0971c, (MTCY10D7.03), len: 269 aa. Probable echA7, enoyl-CoA hydratase, similar to many e.g. CAB95895.1|AL359988 putative enoyl CoA hydratase from Streptomyces coelicolor (247 aa); P24162|ECHH_RHOCA enoyl-CoA hydratase from Rhodobacter capsulatus (257 aa), FASTA scores: opt: 369, E(): 2.6e-15, (33.7% identity in 246 aa overlap); etc. P71540 No lipid metabolism ML0168 MMAR_4536 MSMEG_5495 Mb0996c NC_000962.3 Mycobrowser_v2 CDS 1082584 1083750 . - 0 Rv0972c fadE12 Function unknown, but involved in lipid degradation. Acyl-CoA dehydrogenase FadE12 Rv0972c, (MTCY10D7.02), len: 388 aa. fadE12, acyl-CoA dehydrogenase, highly similar to many e.g. CAB95893.1|AL359988 putative acyl CoA dehydrogenase from Streptomyces coelicolor (382 aa); P45857|ACDB_BACSU from Bacillus subtilis (379 aa), FASTA scores: opt: 576, E(): 2.3e-26, (29.7% identity in 381 aa overlap); etc. P71539 No lipid metabolism ML0167 MMAR_4535 MSMEG_5494 Mb0997c NC_000962.3 Mycobrowser_v2 CDS 1994671 1995054 . - 0 Rv1761c Rv1761c Unknown Possible exported protein Rv1761c, (MTCY28.27c), len: 127 aa. Possibly exported protein with hydrophobic stretch or TMhelix at aa 15-37. O06796 No cell wall and cell processes MMAR_2636 Mb1792c NC_000962.3 Mycobrowser_v2 RBS 1995061 1995063 . - 0 Rv1761c Rv1761c Unknown Possible exported protein Rv1761c, (MTCY28.27c), len: 127 aa. Possibly exported protein with hydrophobic stretch or TMhelix at aa 15-37. O06796 No cell wall and cell processes MMAR_2636 Mb1792c NC_000962.3 Mycobrowser_v2 CDS 1083747 1085750 . - 0 Rv0973c accA2 This protein carries two functions: biotin carboxyl carrier protein and biotin carboxyltransferase. Involved in the first step of long-chain fatty acid synthesis [catalytic activity: ATP + biotin-carboxyl-carrier protein + CO(2) = ADP + phosphate + carboxybiotin-carboxyl-carrier protein]. Probable acetyl-/propionyl-coenzyme A carboxylase alpha chain (alpha subunit) AccA2: biotin carboxylase + biotin carboxyl carrier protein (BCCP) Rv0973c, (MTV044.01c, MTCY10D7.01), len: 667 aa. Probable accA2 (alternate gene name: bccA), acetyl-/propionyl-coenzyme A carboxylase (alpha subunit) [includes: biotin carboxylase ; biotin carboxyl carrier protein (BCCP)], highly similar to others e.g. CAB95892.1|AL359988 putative acetyl/propionyl CoA carboxylase alpha subunit from Streptomyces coelicolor (614 aa); NP_250702.1|NC_002516 probable acyl-CoA carboxylase alpha chain from Pseudomonas aeruginosa (655 aa); NP_420971.1|NC_002696 acetyl/propionyl-CoA carboxylase alpha subunit from Caulobacter crescentus ( 654 aa); NP_251581.1|NC_002516 probable biotin carboxylase/biotin carboxyl carrier protein from Pseudomonas aeruginosa (661 aa); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. Rv2501c|P46401|MTCY07A7.07c|BCCA_MYCTU|ACCA1 probable acetyl-/propionyl-coenzyme A carboxylase alpha chain (alpha subunit) (654 aa), FASTA scores, opt: 250, E(): 4e-09, (28.6% identity in 182 aa overlap); and Rv3285|MTCY71.25|ACCA3 (600 aa); Z83018|MTCY349_20 (1127 aa), FASTA scores: opt: 838, E(): 0, (40.2% identity in 500 aa overlap). Contains PS00867 Carbamoyl-phosphate synthase subdomain signature 2 and PS00188 Biotin-requiring enzymes attachment site. P71538 No lipid metabolism ML0166 MMAR_4534 MSMEG_5493 NC_000962.3 Mycobrowser_v2 RBS 1085759 1085763 . - 0 Rv0973c accA2 This protein carries two functions: biotin carboxyl carrier protein and biotin carboxyltransferase. Involved in the first step of long-chain fatty acid synthesis [catalytic activity: ATP + biotin-carboxyl-carrier protein + CO(2) = ADP + phosphate + carboxybiotin-carboxyl-carrier protein]. Probable acetyl-/propionyl-coenzyme A carboxylase alpha chain (alpha subunit) AccA2: biotin carboxylase + biotin carboxyl carrier protein (BCCP) Rv0973c, (MTV044.01c, MTCY10D7.01), len: 667 aa. Probable accA2 (alternate gene name: bccA), acetyl-/propionyl-coenzyme A carboxylase (alpha subunit) [includes: biotin carboxylase ; biotin carboxyl carrier protein (BCCP)], highly similar to others e.g. CAB95892.1|AL359988 putative acetyl/propionyl CoA carboxylase alpha subunit from Streptomyces coelicolor (614 aa); NP_250702.1|NC_002516 probable acyl-CoA carboxylase alpha chain from Pseudomonas aeruginosa (655 aa); NP_420971.1|NC_002696 acetyl/propionyl-CoA carboxylase alpha subunit from Caulobacter crescentus ( 654 aa); NP_251581.1|NC_002516 probable biotin carboxylase/biotin carboxyl carrier protein from Pseudomonas aeruginosa (661 aa); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. Rv2501c|P46401|MTCY07A7.07c|BCCA_MYCTU|ACCA1 probable acetyl-/propionyl-coenzyme A carboxylase alpha chain (alpha subunit) (654 aa), FASTA scores, opt: 250, E(): 4e-09, (28.6% identity in 182 aa overlap); and Rv3285|MTCY71.25|ACCA3 (600 aa); Z83018|MTCY349_20 (1127 aa), FASTA scores: opt: 838, E(): 0, (40.2% identity in 500 aa overlap). Contains PS00867 Carbamoyl-phosphate synthase subdomain signature 2 and PS00188 Biotin-requiring enzymes attachment site. P71538 No lipid metabolism ML0166 MMAR_4534 MSMEG_5493 NC_000962.3 Mycobrowser_v2 CDS 1085756 1087345 . - 0 Rv0974c accD2 Involved in fatty acid metabolism. Probable acetyl-/propionyl-CoA carboxylase (beta subunit) AccD2 Rv0974c, (MTV044.02c), len: 529 aa. Probable accD2, acetyl-/propionyl-CoA carboxylase (beta subunit), highly similar to many e.g. CAB95891.1|AL35998 putative acetyl/propionyl CoA carboxylase beta subunit from Streptomyces coelicolor (532 aa); NP_250704.1|NC_002516 probable acyl-CoA carboxyltransferase beta chain from Pseudomonas aeruginosa (535 aa); BAB16296.1|AB039884 acetyl-CoA carboxylase carboxyltransferase from Myxococcus xanthus (538 aa); NP_420973.1|NC_002696 putative propionyl-CoA carboxylase beta subunit from Caulobacter crescentus (530 aa); etc. Also similar to other from Mycobacterium tuberculosis: Rv2502c|ACCD1, Rv3799c|ACCD4, etc. Could belong to the ACCD/PCCB family. O86318 No lipid metabolism ML0165 MMAR_4533 MSMEG_5492 Mb0999c NC_000962.3 Mycobrowser_v2 CDS 2205277 2205549 . - 0 Rv1962A vapB35 Unknown Possible antitoxin VapB35 Rv1962A, len: 90 aa. Possible vapB35, antitoxin, part of toxin-antitoxin (TA) operon with Rv1962c, see Arcus et al. 2005. Similar to others in M. tuberculosis e.g. Rv3385c, Rv3407, Rv0626 P0CW28 No virulence, detoxification, adaptation NC_000962.3 Mycobrowser_v2 CDS 1087348 1088496 . - 0 Rv0975c fadE13 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE13 Rv0975c, (MTV044.03c), len: 382 aa. Probable fadE13, acyl-CoA dehydrogenase, highly similar to many e.g. T35427 probable acyl-CoA dehydrogenase from Streptomyces coelicolor (382 aa); M74096|HUMACADL_1 Human long chain acyl-CoA dehydrogenase from Homo sapiens (430 aa), FASTA scores: opt: 819, E(): 0, (37.0% identity in 376 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. fadE20|Z98209|MTCY154_4 (386 aa), FASTA scores: (40.3% identity in 375 aa overlap). Contains PS00073 Acyl-CoA dehydrogenases signature 2. Belongs to the acyl-CoA dehydrogenases family. O86319 No lipid metabolism ML0164 MMAR_4532 MSMEG_5491 Mb1000c NC_000962.3 Mycobrowser_v2 CDS 1088493 1090175 . - 0 Rv0976c Rv0976c Function unknown Conserved hypothetical protein Rv0976c, (MTV044.04c), len: 560 aa. Conserved hypothetical protein, highly similar to others e.g. CAB95890.1|AL359988 conserved hypothetical protein from Streptomyces coelicolor (558 aa); P_251576.1|NC_002516 hypothetical protein from Pseudomonas aeruginosa (600 aa); etc. N-terminal part highly similar to AL035500|MLCL373_14 probable pseudogene from Mycobacterium leprae (163 aa), FASTA score: (50.0% identity in 122 aa overlap). O86320 No conserved hypotheticals MMAR_4531 MSMEG_5490 Mb1001c NC_000962.3 Mycobrowser_v2 CDS 1146561 1147028 . + 0 Rv1025 Rv1025 Function unknown Conserved protein Rv1025, (MTCY10G2.24c), len: 155 aa. Conserved protein, similar to hypothetical protein AE001768|AE001768_4 Thermotoga maritima (170 aa) FASTA scores: opt: 254, E(): 9.5e-10, (35.7% identity in 143 aa overlap). P96375 No conserved hypotheticals ML0257 MMAR_4460 Mb1053 NC_000962.3 Mycobrowser_v2 RBS 1090359 1090364 . + 0 Rv0977 PE_PGRS16 Function unknown PE-PGRS family protein PE_PGRS16 Rv0977, (MTV044.05), len: 923 aa. PE_PGRS16, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to other PGRS-type sequences e.g. AL0091|MTV004_1 from Mycobacterium tuberculosis (1125 aa), FASTA score: (45.4% identity in 959 aa overlap); Z80225|MTCY441_4 from Mycobacterium tuberculosis (778 aa), FASTA score: (51.5% identity in 750 aa overlap); etc. Q79FU3 No PE/PPE Mb1002 NC_000962.3 Mycobrowser_v2 CDS 1090373 1093144 . + 0 Rv0977 PE_PGRS16 Function unknown PE-PGRS family protein PE_PGRS16 Rv0977, (MTV044.05), len: 923 aa. PE_PGRS16, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to other PGRS-type sequences e.g. AL0091|MTV004_1 from Mycobacterium tuberculosis (1125 aa), FASTA score: (45.4% identity in 959 aa overlap); Z80225|MTCY441_4 from Mycobacterium tuberculosis (778 aa), FASTA score: (51.5% identity in 750 aa overlap); etc. Q79FU3 No PE/PPE Mb1002 NC_000962.3 Mycobrowser_v2 CDS 1093361 1094356 . - 0 Rv0978c PE_PGRS17 Function unknown PE-PGRS family protein PE_PGRS17 Rv0978c, (MTV044.06c), len: 331 aa. PE_PGRS17, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to others e.g. Z95387|MTCY1A10_19 from Mycobacterium tuberculosis (461 aa), FASTA score: (73.6% identity in 277 aa overlap); etc. Q79FU2 No PE/PPE Mb1003c NC_000962.3 Mycobrowser_v2 RBS 1094362 1094368 . - 0 Rv0978c PE_PGRS17 Function unknown PE-PGRS family protein PE_PGRS17 Rv0978c, (MTV044.06c), len: 331 aa. PE_PGRS17, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to others e.g. Z95387|MTCY1A10_19 from Mycobacterium tuberculosis (461 aa), FASTA score: (73.6% identity in 277 aa overlap); etc. Q79FU2 No PE/PPE Mb1003c NC_000962.3 Mycobrowser_v2 CDS 1094670 1094864 . - 0 Rv0979c Rv0979c Unknown Hypothetical protein Rv0979c, (MTV044.07c), len: 64 aa (unlikely ORF). Hypothetical unknown protein. Start codon changed since first submission (-44 aa). O53892 No conserved hypotheticals Mb1004c NC_000962.3 Mycobrowser_v2 RBS 1095003 1095006 . - 0 Rv0979c Rv0979c Unknown Hypothetical protein Rv0979c, (MTV044.07c), len: 64 aa (unlikely ORF). Hypothetical unknown protein. Start codon changed since first submission (-44 aa). O53892 No conserved hypotheticals Mb1004c NC_000962.3 Mycobrowser_v2 RBS 1094872 1094876 . + 0 Rv0979A rpmF Involved in translation mechanism. 50S ribosomal protein L32 RpmF Rv0979A, len: 57 aa. rpmF, 50S ribosomal protein L32, similar to others e.g. rpmF|Q9RL50 probable 50S ribosomal protein from Streptomyces coelicolor (56 aa), FASTA scores: E(): 5.1e-09, (63.45% identity in 52 aa overlap); etc. Belongs to the L32P family of ribosomal proteins. P0A5V8 No information pathways ML0173 MMAR_4530 MSMEG_5489 Mb1005 NC_000962.3 Mycobrowser_v2 CDS 1094886 1095059 . + 0 Rv0979A rpmF Involved in translation mechanism. 50S ribosomal protein L32 RpmF Rv0979A, len: 57 aa. rpmF, 50S ribosomal protein L32, similar to others e.g. rpmF|Q9RL50 probable 50S ribosomal protein from Streptomyces coelicolor (56 aa), FASTA scores: E(): 5.1e-09, (63.45% identity in 52 aa overlap); etc. Belongs to the L32P family of ribosomal proteins. P0A5V8 No information pathways ML0173 MMAR_4530 MSMEG_5489 Mb1005 NC_000962.3 Mycobrowser_v2 CDS 1095078 1096451 . - 0 Rv0980c PE_PGRS18 Function unknown PE-PGRS family protein PE_PGRS18 Rv0980c, (MTV044.08c), len: 457 aa. PE_PGRS18, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002), highly similar to others e.g. Z95387|MTCY1A10_19 from Mycobacterium tuberculosis (461 aa), FASTA score: (66.7% identity in 405 aa overlap); Z95844|MTCY493_2 from Mycobacterium tuberculosis (741 aa), FASTA score: (53.0% identity in 394 aa overlap); etc. Predicted to be an outer membrane protein (See Song et al., 2008). Q79FU0 No PE/PPE Mb1006c NC_000962.3 Mycobrowser_v2 RBS 1096457 1096463 . - 0 Rv0980c PE_PGRS18 Function unknown PE-PGRS family protein PE_PGRS18 Rv0980c, (MTV044.08c), len: 457 aa. PE_PGRS18, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002), highly similar to others e.g. Z95387|MTCY1A10_19 from Mycobacterium tuberculosis (461 aa), FASTA score: (66.7% identity in 405 aa overlap); Z95844|MTCY493_2 from Mycobacterium tuberculosis (741 aa), FASTA score: (53.0% identity in 394 aa overlap); etc. Predicted to be an outer membrane protein (See Song et al., 2008). Q79FU0 No PE/PPE Mb1006c NC_000962.3 Mycobrowser_v2 CDS 1108578 1109171 . - 0 Rv0992c Rv0992c Function unknown Conserved hypothetical protein Rv0992c, (MTCI237.06c), len: 197 aa. Conserved hypothetical protein, equivalent to NP_301256.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (197 aa). Also similar, except in N-terminus, to other hypothetical proteins and ligases e.g. SCE87.34|CAB59679.1|AL132674 hypothetical protein from Streptomyces coelicolor (204 aa); NP_461977.1|NC_003197 putative ligase from Salmonella typhimurium (182 aa); P09160|YGFA_ECOLI hypothetical 21.1 kDa protein from Escherichia coli (182 aa), FASTA scores: opt: 191, E(): 1.1e-09, (29.5% identity in 146 aa overlap); etc. O05575 No conserved hypotheticals ML0181 MMAR_4522 MSMEG_5472 Mb1019c NC_000962.3 Mycobrowser_v2 CDS 1096822 1097508 . + 0 Rv0981 mprA Regulator part of a two component regulatory system (MPRAB system) Mycobacterial persistence regulator MRPA (two component response transcriptional regulatory protein) Rv0981, (MTV044.09), len: 228 aa. MprA, mycobacterial persistence regulator, a two-component response regulator whose expression is required for entrance into and maintenance of persistent infection (see citation below), equivalent to NP_301250.1|NC_002677 putative two-component response regulator from Mycobacterium leprae (228 aa); and highly similar to others from Mycobacterium leprae. Also highly similar to others e.g. AAG36759.1|AF119221_1|AF119221 response regulator from Corynebacterium glutamicum (232 aa); CAB88489.1|AL353816 putative two-component system response regulator from Streptomyces coelicolor (248 aa); BJY09666_1 two-component response regulator (ragA, ragB and rpoH3) from B.japonicum (226 aa), FASTA score: (43.8% identity in 224 aa overlap); BSAJ2571_44 two-component response regulator from Bacillus subtilis (228 aa), FASTA score: (46.4% identity in 224 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. Rv1033c (257 aa); Rv0903c (236 aa), FASTA score: (50.7 identity in 225 aa overlap); etc. Contains PS00217 Sugar transport proteins signature 2. Start changed since first submission (-2 aa). MprAB is involved in the regulation of genes in response to environmental stress (See He et al., 2006). O53894 No regulatory proteins ML0174 MMAR_4529 MSMEG_5488 Mb1007 NC_000962.3 Mycobrowser_v2 CDS 1097508 1099022 . + 0 Rv0982 mprB Sensor part of a two component regulatory system (MPRAB system) Two component sensor kinase MprB Rv0982, (MTV044.10), len: 504 aa. MprB, two component sensor kinase, probable transmembrane protein (see citation below), equivalent to AL035500|MLCL373_16|NP_301251.1|NC_002677 putative two-component system sensor kinase from Mycobacterium leprae (519 aa), FASTA score: (81.0% identity in 521 aa overlap). Also highly similar to others (especially in C-terminal part) e.g. AAG36760.1|AF119221_2|AF119221 sensor kinase from Corynebacterium glutamicum (455 aa); CAB89748.1|AL354616 putative two-component histidine kinase from Streptomyces coelicolor (481 aa); X58793|SLCUTRS_2 sensor kinase from S.lividans (414 aa), FASTA scores: opt: 451, E(): 4.2e-21, (36.0% identity in 303 aa overlap); P30847|BAES_ECOLI sensor protein from Escherichia coli (467 aa), FASTA scores: opt: 412, E(): 1.3e-18, (30.4% identity in 336 aa overlap); etc. Also similar in C-terminal region to C-terminus of Rv0902c|Z73101|MTCY31_33 from Mycobacterium tuberculosis (446 aa), FASTA scores: opt: 423, E(): 2.6e-19, (28.4 identity in 462 aa overlap). MprAB is involved in the regulation of genes in response to environmental stress (See He et al., 2006). O53895 No regulatory proteins ML0175 MMAR_4528 MSMEG_5487 Mb1008 NC_000962.3 Mycobrowser_v2 CDS 1099066 1100460 . + 0 Rv0983 pepD Function unknown; possibly hydrolyzes peptides and/or proteins (seems to cleave preferentially after serine residues). Probable serine protease PepD (serine proteinase) (MTB32B) Rv0983, (MTV044.11), len: 464 aa. Probable pepD (alternate gene name: mtb32b), secreted or membrane serine protease (see citation below), equivalent (but longer 18 aa in N-terminus) to AL035500|MLCL373_17|T45448 probable serine proteinase from Mycobacterium leprae (452 aa), FASTA score: (74.2% identity in 466 aa overlap); and highly similar to others from Mycobacterium leprae. Also highly similar (except in N-terminus) to other proteases e.g. CAC01350.1|AL390975 putative protease from Streptomyces coelicolor (542 aa); NP_440705.1|NC_000911|HtrA serine protease from Synechocystis sp. (452 aa); NP_346646.1|NC_003028 serine protease from Streptococcus pneumoniae (393 aa); etc. Also similar in part to members of the htrA-antigen family e.g. U87242|MTU87242_3|HtrA serine protease from M. tuberculosis (542 aa), FASTA scores: opt: 846, E(): 2e-28, (40.6% identity in 392 aa overlap); and similar to other hypothetical serine proteases e.g. Rv0983, Rv0125, etc. Belongs to the serine protease family. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). O53896 No intermediary metabolism and respiration ML0176 MMAR_4527 MSMEG_5486 Mb1009 NC_000962.3 Mycobrowser_v2 CDS 1162549 1163376 . - 0 Rv1040c PE8 Function unknown PE family protein PE8 Rv1040c, (MTCY10G2.09), len: 275 aa. PE8, Member of the Mycobacterium tuberculosis PE family (see citation below), most similar to AL008967|MTV002_34 Mycobacterium tuberculosis H37Rv (275 aa), FASTA scores: opt: 1111, E(): 0, (68.6% identity in 283 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q7D8Y6 No PE/PPE ML0263 MMAR_4451 Mb1069c NC_000962.3 Mycobrowser_v2 RBS 1100451 1100454 . + 0 Rv0984 moaB2 Thought to be involved in molybdopterin biosynthesis. Catalyzes the dehydratation of 4A-hydroxytetrahydropterins [catalytic activity: (6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4A-hydroxypterin = (6R)-6-(L-erythro-1,2- dihydroxypropyl)-7,8-dihydro-6H-pterin + H(2)O.]. Possible pterin-4-alpha-carbinolamine dehydratase MoaB2 (PHS) (4-alpha- hydroxy-tetrahydropterin dehydratase) (pterin-4-a-carbinolamine dehydratase) (phenylalanine hydroxylase-stimulating protein) (PHS) (pterin carbinolamine dehydratase) (PCD) Rv0984, (MTV044.12), len: 181 aa. Possible moaB2, pterin-4-alpha-carbinolamine dehydratase, highly similar to NP_301253.1|NC_002677 putative molybdenum cofactor biosynthesis protein from Mycobacterium leprae (181 aa), FASTA score: (92.3% identity in 181 aa overlap). Also similar to others e.g. CAB59675.1|AL132674 molybdenum cofactor biosynthesis protein from Streptomyces coelicolor (179 aa); Q56208|MOCB_SYNP7 molybdenum cofactor biosynthesis protein CB from Synechococcus sp. (319 aa), FASTA score: (37.3% identity in 142 aa overlap); C-terminus of NP_197599.1|NC_003076 molybdopterin biosynthesis CNX1 protein from Arabidopsis thaliana (670 aa); etc. Also similar to Rv0865|MOG from Mycobacterium tuberculosis (160 aa); and other mog proteins e.g. CAC39235.1|AJ312124 Mog protein from Eubacterium acidaminophilum (162 aa). Could belong to the pterin-4-alpha-carbinolamine dehydratase family. Alternative start codon has been suggested in position 1100508. O53897 No intermediary metabolism and respiration ML0177 MSMEG_5485 Mb1010 NC_000962.3 Mycobrowser_v2 CDS 1100460 1101005 . + 0 Rv0984 moaB2 Thought to be involved in molybdopterin biosynthesis. Catalyzes the dehydratation of 4A-hydroxytetrahydropterins [catalytic activity: (6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4A-hydroxypterin = (6R)-6-(L-erythro-1,2- dihydroxypropyl)-7,8-dihydro-6H-pterin + H(2)O.]. Possible pterin-4-alpha-carbinolamine dehydratase MoaB2 (PHS) (4-alpha- hydroxy-tetrahydropterin dehydratase) (pterin-4-a-carbinolamine dehydratase) (phenylalanine hydroxylase-stimulating protein) (PHS) (pterin carbinolamine dehydratase) (PCD) Rv0984, (MTV044.12), len: 181 aa. Possible moaB2, pterin-4-alpha-carbinolamine dehydratase, highly similar to NP_301253.1|NC_002677 putative molybdenum cofactor biosynthesis protein from Mycobacterium leprae (181 aa), FASTA score: (92.3% identity in 181 aa overlap). Also similar to others e.g. CAB59675.1|AL132674 molybdenum cofactor biosynthesis protein from Streptomyces coelicolor (179 aa); Q56208|MOCB_SYNP7 molybdenum cofactor biosynthesis protein CB from Synechococcus sp. (319 aa), FASTA score: (37.3% identity in 142 aa overlap); C-terminus of NP_197599.1|NC_003076 molybdopterin biosynthesis CNX1 protein from Arabidopsis thaliana (670 aa); etc. Also similar to Rv0865|MOG from Mycobacterium tuberculosis (160 aa); and other mog proteins e.g. CAC39235.1|AJ312124 Mog protein from Eubacterium acidaminophilum (162 aa). Could belong to the pterin-4-alpha-carbinolamine dehydratase family. Alternative start codon has been suggested in position 1100508. O53897 No intermediary metabolism and respiration ML0177 MSMEG_5485 Mb1010 NC_000962.3 Mycobrowser_v2 RBS 3478767 3478770 . + 0 Rv0984 moaB2 Thought to be involved in molybdopterin biosynthesis. Catalyzes the dehydratation of 4A-hydroxytetrahydropterins [catalytic activity: (6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4A-hydroxypterin = (6R)-6-(L-erythro-1,2- dihydroxypropyl)-7,8-dihydro-6H-pterin + H(2)O.]. Possible pterin-4-alpha-carbinolamine dehydratase MoaB2 (PHS) (4-alpha- hydroxy-tetrahydropterin dehydratase) (pterin-4-a-carbinolamine dehydratase) (phenylalanine hydroxylase-stimulating protein) (PHS) (pterin carbinolamine dehydratase) (PCD) Rv0984, (MTV044.12), len: 181 aa. Possible moaB2, pterin-4-alpha-carbinolamine dehydratase, highly similar to NP_301253.1|NC_002677 putative molybdenum cofactor biosynthesis protein from Mycobacterium leprae (181 aa), FASTA score: (92.3% identity in 181 aa overlap). Also similar to others e.g. CAB59675.1|AL132674 molybdenum cofactor biosynthesis protein from Streptomyces coelicolor (179 aa); Q56208|MOCB_SYNP7 molybdenum cofactor biosynthesis protein CB from Synechococcus sp. (319 aa), FASTA score: (37.3% identity in 142 aa overlap); C-terminus of NP_197599.1|NC_003076 molybdopterin biosynthesis CNX1 protein from Arabidopsis thaliana (670 aa); etc. Also similar to Rv0865|MOG from Mycobacterium tuberculosis (160 aa); and other mog proteins e.g. CAC39235.1|AJ312124 Mog protein from Eubacterium acidaminophilum (162 aa). Could belong to the pterin-4-alpha-carbinolamine dehydratase family. Alternative start codon has been suggested in position 1100508. O53897 No intermediary metabolism and respiration ML0177 MSMEG_5485 Mb1010 NC_000962.3 Mycobrowser_v2 CDS 1101025 1101480 . - 0 Rv0985c mscL Ion channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell. Possible large-conductance ion mechanosensitive channel MscL Rv0985c, (MTV044.13c), len: 151 aa. Possible mscL, large conductance mechanosensitive ion channel (integral membrane protein) (see citations below, equivalent to AL035500|MLCL373_19|NP_301254.1|NC_002677 putative mechanosensitive channel protein from Mycobacterium leprae (154 aa), FASTA score: (71.0% identity in 155 aa overlap). Also highly similar to others e.g. NP_268999.1|NC_002737 putative large conductance mechanosensitive channel from Streptococcus pyogenes (120 aa); CAB90974.1|AL355832 putative mechanosensitive channel from Streptomyces coelicolor (156 aa); Q9X722|MSCL_CLOHI large-conductance mechanosensitive channel from Clostridium histolyticum (133 aa); Z83337|BSZ83337_6 large conductance mechanosensitive channel from Bacillus subtilis (130 aa), FASTA scores: opt: 248, E(): 8.4e-10, (39.0% identity in 136 aa overlap); U08371|ECU08371_1 large conductance mechanosensitive channel from Escherichia coli strain K-12 (136 aa), FASTA score: (36.6% identity in 134 aa overlap); etc. Belongs to the MscL family. P0A5K8 No cell wall and cell processes ML0178 MMAR_4525 MSMEG_5482 Mb1011c NC_000962.3 Mycobrowser_v2 RBS 1101793 1101799 . + 0 Rv0986 Rv0986 Thought to be involved in active transport of adhesion component across the membrane: involved in attachment and virulence. Responsible for energy coupling to the transport system. Probable adhesion component transport ATP-binding protein ABC transporter Rv0986, (MTV044.14), len: 248 aa. Probable ATP-binding protein ABC transporter supposedly involved in transport of adhesion component (see citation below), highly similar to many ATP-binding proteins e.g. AE0010|AE001033_8 ABC transporter ATP-binding protein from Archaeoglobus fulgidus (228 aa), FASTA scores: opt: 669, E(): 0, (45.7% identity in 219 aa overlap); CAB81857.1|AL161691 putative ABC-transporter ATP-binding protein from Streptomyces coelicolor (246 aa); X84019|ZMDNAGRP_4 glutamate uptake regulatory protein (grp) from Z.mobilis (232 aa), FASTA score: (44.4% identity in 225 aa overlap); Z99111|BSUB0008_108 from Bacillus subtilis (230 aa), FASTA score: (38.7% identity in 222 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). Believed to have been acquired by horizontal gene transfer (See Rosas-Magallanes et el., 2006; Becq et al., 2007). O53899 No cell wall and cell processes Mb1012 NC_000962.3 Mycobrowser_v2 CDS 1101803 1102549 . + 0 Rv0986 Rv0986 Thought to be involved in active transport of adhesion component across the membrane: involved in attachment and virulence. Responsible for energy coupling to the transport system. Probable adhesion component transport ATP-binding protein ABC transporter Rv0986, (MTV044.14), len: 248 aa. Probable ATP-binding protein ABC transporter supposedly involved in transport of adhesion component (see citation below), highly similar to many ATP-binding proteins e.g. AE0010|AE001033_8 ABC transporter ATP-binding protein from Archaeoglobus fulgidus (228 aa), FASTA scores: opt: 669, E(): 0, (45.7% identity in 219 aa overlap); CAB81857.1|AL161691 putative ABC-transporter ATP-binding protein from Streptomyces coelicolor (246 aa); X84019|ZMDNAGRP_4 glutamate uptake regulatory protein (grp) from Z.mobilis (232 aa), FASTA score: (44.4% identity in 225 aa overlap); Z99111|BSUB0008_108 from Bacillus subtilis (230 aa), FASTA score: (38.7% identity in 222 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). Believed to have been acquired by horizontal gene transfer (See Rosas-Magallanes et el., 2006; Becq et al., 2007). O53899 No cell wall and cell processes Mb1012 NC_000962.3 Mycobrowser_v2 CDS 1102542 1105109 . + 0 Rv0987 Rv0987 Thought to be involved in active transport of adhesion component across the membrane: involved in atachment and virulence. Responsible for the translocation of the substrate across the membrane. Probable adhesion component transport transmembrane protein ABC transporter Rv0987, (MTV044.15, MTCI237.01), len: 855 aa. Probable transmembrane protein ABC transporter supposedly involved in transport of adhesion component (see citation below), whose N-terminus shows similarity with hypothetical proteins, generally transmembrane proteins, e.g. CAB96016.1|AL360055 putative ABC transport system integral membrane protein from Streptomyces coelicolor (855 aa); P44252|YCFU_HAEIN|HI1555 hypothetical protein from Haemophilus influenzae (393 aa), FASTA scores: opt: 265, E(): 1.7e-09, (23.6% identity in 402 aa overlap); etc. N- and C-termini respectively show similarity to O32735 ATTF protein (420 aa), FASTA scores: E(): 1e-09, (26.7% identity in 430 aa overlap), and G2340078 ATTG protein (359 aa), FASTA scores: E(): 2.7e-08, (27.8% identity in 356 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Believed to have been acquired by horizontal gene transfer (See Rosas-Magallanes et el., 2006; Becq et al., 2007). O53900 No cell wall and cell processes Mb1013,Mb1014 NC_000962.3 Mycobrowser_v2 CDS 1134785 1135465 . - 0 Rv1016c lpqT Unknown Probable conserved lipoprotein LpqT Rv1016c, (MTCY10G2.33), len: 226 aa. Probable lpqT, conserved lipoprotein. Similar to several Mycobacterium tuberculosis hypothetical proteins e.g. Rv0040c|Y0H3_MYCTU|P71697 Proline rich 28 kDA antigen (310 aa), FASTA scores: opt: 329, E(): 2e-17, (32.3% identity in 229 aa overlap); Rv0583c. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P96384 No cell wall and cell processes ML0246 MMAR_4470 MSMEG_5429 Mb1044c NC_000962.3 Mycobrowser_v2 CDS 1105116 1106276 . + 0 Rv0988 Rv0988 Unknown Possible conserved exported protein Rv0988, (MTCI237.02), len: 386 aa. Possible conserved exported protein, with potential N-terminal signal sequence, similar (except in N-terminus) to O32737|L63540 ATTH protein from Agrobacterium tumefaciens (355 aa), FASTA scores: opt: 651, E(): 5.7e-33, (33.4% identity in 344 aa overlap); and NP_231265.1|NC_002505 conserved hypothetical protein from Vibrio cholerae (372 aa). Predicted to be an outer membrane protein (See Song et al., 2008). Believed to have been acquired by horizontal gene transfer (See Rosas-Magallanes et el., 2006; Becq et al., 2007). O86370 No cell wall and cell processes Mb1015 NC_000962.3 Mycobrowser_v2 CDS 1106405 1107382 . - 0 Rv0989c grcC2 Possible supplier of polyprenyl diphosphate. Probable polyprenyl-diphosphate synthase GrcC2 (polyprenyl pyrophosphate synthetase) Rv0989c, (MTCI237.03c), len: 325 aa. Probable grcC2, polyprenyl diphosphate synthetase, highly similar to NP_302483.1|NC_002677 polyprenyl diphosphate synthase component from Mycobacterium leprae (330 aa). Also similar to others (generally hepta or hexaprenyl e.g. NP_471378.1|NC_003212 protein similar to heptaprenyl diphosphate synthase component II (menaquinone biosynthesis) from Listeria innocua (321 aa); NP_371994.1|NC_002758 heptaprenyl diphosphate syntase component II from Staphylococcus aureus subsp. aureus Mu50 (319 aa); P55785|HEP2_BACST heptaprenyl diphosphate synthase component from Bacillus subtilis (323 aa), FASTA scores: opt: 496, E(): 1.4e-24, (31.4% identity in 306 aa overlap); etc. Also highly similar to Mycobacterium tuberculosis proteins e.g. Rv0562|grcC1|NP_215076.1|MTCY25D10.41 probable polyprenyl-diphosphate synthase (335 aa); Rv3383, Rv3398c, Rv2173, etc. Seems to belong to the FPP/GGPP synthetases family. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O05572 No intermediary metabolism and respiration Mb1016c NC_000962.3 Mycobrowser_v2 CDS 1107443 1108099 . - 0 Rv0990c Rv0990c Unknown Hypothetical protein Rv0990c, (MTCI237.04c), len: 218 aa. Hypothetical unknown protein. O05573 No conserved hypotheticals ML0179 MMAR_4524 MSMEG_5481 Mb1017c NC_000962.3 Mycobrowser_v2 CDS 1109272 1110192 . + 0 Rv0993 galU May play a role in stationary phase survival [catalytic activity: UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose]. UTP--glucose-1-phosphate uridylyltransferase GalU (UDP-glucose pyrophosphorylase) (UDPGP) (alpha-D-glucosyl-1-phosphate uridylyltransferase) (uridine diphosphoglucose pyrophosphorylase) Rv0993, (MTCI237.07), len: 306 aa. GalU, UTP--glucose-1-phosphate uridylyltransferase, equivalent to AL035500|MLCL373_22 putative UTP-glucose-1-phosphate uridylyltransferase from Mycobacterium leprae (306 aa), FASTA score: (89.7% identity in 302 aa overlap). Also highly similar to others e.g. AB59678.1|AL132674 UTP-glucose-1-phosphate uridylyltransferase from Streptomyces coelicolor (303 aa); NP_244519.1|NC_002570 UTP-glucose-1-phosphate uridylyltransferase from Bacillus halodurans (297 aa); P25520|GALU_ECOLI|B1236|Z2012|ECS17 UTP--glucose-1-phosphate uridylyltransferase from Escherichia coli strains K12 and O157:H7 (301 aa), FASTA scores: opt: 624, E(): 2.4e-33, (38.8% identity in 299 aa overlap); etc. Belongs to the prokaryotic UDPGP family. O05576 No intermediary metabolism and respiration ML0182 MMAR_4521 MSMEG_5471 Mb1020 NC_000962.3 Mycobrowser_v2 CDS 1110269 1111549 . + 0 Rv0994 moeA1 Involved in molybdenum cofactor biosynthesis: involved in the biosynthesis of a demolybdo-cofactor (molybdopterin), necessary for molybdo-enzymes. Probable molybdopterin biosynthesis protein MoeA1 Rv0994, (MTCI237.08), len: 426 aa. Probable moeA1, molybdenum cofactor biosynthesis protein, equivalent to AL035500|MLCL373_23 putative molybdopterin biosynthesis protein from Mycobacterium leprae (424 aa), FASTA score: (88.3% identity in 426 aa overlap). Also highly similar to many e.g. CAB59677.1|AL132674 molybdopterin biosynthesis protein from Streptomyces coelicolor (424 aa); NP_385769.1|NC_003047 probable molybdopterin biosynthesis protein from Sinorhizobium meliloti (406 aa); P12281|MOEA_ECOLI molybdopterin biosynthesis moea protein from Escherichia coli (411 aa), FASTA scores: opt: 519, E(): 1.3e-24, (32.3% identity in 402 aa overlap); etc. Also similar to MOEA2|Rv0438c|MTV037.02c probable molybdopterin biosynthesis protein from Mycobacterium tuberculosis (405 aa). Note that previously known as moeA. O05577 No intermediary metabolism and respiration ML0183 MMAR_4520 MSMEG_5470 Mb1021 NC_000962.3 Mycobrowser_v2 ncRNA 1175225 1175315 . + 0 MTB000061 mpr5 Unknown Fragment of putative small regulatory RNA mpr5, fragment of putative small regulatory RNA (See DiChiara et al., 2010), ends not mapped, ~100 nt band detected by Northern blot. No stable RNAs NC_000962.3 Mycobrowser_v2 CDS 1160855 1161151 . - 0 Rv1038c esxJ Function unknown ESAT-6 like protein EsxJ (ESAT-6 like protein 2) Rv1038c, (MT1067, MTCY10G2.11), len: 98 aa. EsxJ, ESAT-6 like protein (see Gey Van Pittius et al., 2001), similar to Q49945|U1756C, Mycobacterium leprae (100 aa), FASTA scores: opt: 375, E(): 7.7e-21, (58.3% identity in 96 aa overlap). Member of M. tuberculosis hypothetical QILSS protein family with Rv1197, Rv1792, Rv2347c and Rv3620c. Belongs to the ESAT6 family. P96363 No cell wall and cell processes ML0262 MMAR_2674,MMAR_3654,MMAR_3663,MMAR_4453,MMAR_5120 Mb1067c NC_000962.3 Mycobrowser_v2 CDS 1111612 1112223 . + 0 Rv0995 rimJ Acetylates the N-terminal alanine of ribosomal protein S5 [catalytic activity: acetyl-CoA + ribosomal-protein L-alanine = CoA + ribosomal-protein N-acetyl-L-alanine]. Ribosomal-protein-alanine acetyltransferase RimJ (acetylating enzyme for N-terminal of ribosomal protein S5) Rv0995, (MTCI237.09), len: 203 aa. RimJ, ribosomal-protein-alanine acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Equivalent to AL035500|MLCL373_24 probable acyltransferase from Mycobacterium leprae (218 aa), FASTA scores: (86.0% identity in 200 aa overlap). Also similar to others and many acyltransferases e.g. BAB69252.1|AB070946 possible acyltransferase from Streptomyces avermitilis (156 aa); NP_385025.1|NC_003047 probable ribosomal-protein-alanine acetyltransferase from Sinorhizobium meliloti (203 aa); P09454|RIMJ_ECOLI|B1066|Z1703|ECS1444 ribosomal-protein-alanine acetyltransferase from Escherichia coli strains K12 and O157:H7 (194 aa), FASTA scores: opt: 247, E(): 1.5e-10, (26.9% identity in 186 aa overlap). Belongs to the acetyltransferase family, RIMJ subfamily. O05578 No information pathways ML0184 MMAR_4519 MSMEG_5469 Mb1022 NC_000962.3 Mycobrowser_v2 CDS 1112384 1113460 . + 0 Rv0996 Rv0996 Unknown Probable conserved transmembrane protein Rv0996, (MTCI237.10), len: 358 aa. Probable conserved transmembrane protein, equivalent to AL035500|MLCL373_25 putative membrane protein from Mycobacterium leprae (342 aa), FASTA scores: (66.4% identity in 360 aa overlap). Contains possible signal sequence and other hydrophobic domains. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O05579 No cell wall and cell processes ML0185 MSMEG_5468 Mb1023 NC_000962.3 Mycobrowser_v2 CDS 1114293 1114724 . + 0 Rv0997 Rv0997 Unknown Hypothetical protein Rv0997, (MTCI237.11), len: 143 aa. Hypothetical unknown protein, equivalent to AAK45276.1 from Mycobacterium tuberculosis strain CDC1551 (87 aa) but longer 56 aa. O05580 No conserved hypotheticals ML0186 Mb1024 NC_000962.3 Mycobrowser_v2 CDS 1122222 1123598 . - 0 Rv1005c pabB Catalyzes the biosynthesis of 4-amino-4-deoxychorismate (ADC) from chorismate and glutamine Probable para-aminobenzoate synthase component I PABD Rv1005c, (MTCI237.22c), len: 458 aa (Start-site not certain). Probable PabD, para-aminobenzoate synthase component I. Similar to PABB_ECOLI|P05041 para-aminobenzoate synthase component I from Escherichia coli (453 aa), FASTA scores: opt: 589, E(): 1.8e-27, (40.7% identity in 268 aa overlap). Similar to M. tuberculosis Rv1609, Rv3215, Rv2386c. O05591 No intermediary metabolism and respiration ML0236 MMAR_4488 MSMEG_5446 Mb1032c NC_000962.3 Mycobrowser_v2 CDS 2258030 2258272 . + 0 Rv2009 vapB15 Unknown Antitoxin VapB15 Rv2009, (MTCY39.08c), len: 80 aa. VapB15, antitoxin, part of toxin-antitoxin (TA) operon with Rv2010 (See Arcus et al., 2005; Pandey and Gerdes, 2005). Q10848 No virulence, detoxification, adaptation Mb2032 NC_000962.3 Mycobrowser_v2 CDS 1114748 1115749 . + 0 Rv0998 Rv0998 Unknown Conserved hypothetical protein Rv0998, (MTCI237.12), len: 333 aa. Conserved hypothetical protein, with cyclic nucleotide-binding domain in N-terminal part and GNAT (Gcn5-related N-acetyltransferase) domain in C-terminal part. See Vetting et al. 2005. Possibly cyclic nucleotide-dependent protein kinase, highly similar to NP_301261.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (353 aa); and AL035500|MLCL373.38|T45457 hypothetical protein from Mycobacterium leprae (143 aa), FASTA score: (61.5% identity in 143 aa overlap). Also similar to many hypothetical proteins and cyclic-NMP-dependent protein kinases (generally at C-terminus) e.g. N-terminus of SC9B10.09|T35878 hypothetical protein from Streptomyces coelicolor (1039 aa); P05987|KAPR_DICDI camp-dependent protein kinase regulatory chain from Dictyostelium discoideum (327 aa), FASTA scores: opt: 177, E(): 0.00036, (32.0% identity in 122 aa overlap); NP_104403.1|NC_002678 hypothetical protein (contains similarity to cAMP-dependent protein kinase regulatory subunit) from Mesorhizobium loti (151 aa); etc. Contains PS00889 Cyclic nucleotide-binding domain signature 2. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O05581 No conserved hypotheticals ML0187 MMAR_4496 MSMEG_5458 Mb1025 NC_000962.3 Mycobrowser_v2 CDS 1115767 1116525 . + 0 Rv0999 Rv0999 Unknown Unknown protein Rv0999, (MTCI237.13), len: 252 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). O05582 No conserved hypotheticals ML0189 MMAR_4494 MSMEG_5452 Mb1026 NC_000962.3 Mycobrowser_v2 CDS 1116531 1117148 . - 0 Rv1000c Rv1000c Function unknown Conserved hypothetical protein Rv1000c, len: 205 aa. Conserved hypothetical protein, equivalent to ML0190|NP_301263.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (205 aa). Also highly similar to SC5F8.12c|CAB93740.1|AL357613 hypothetical protein from Streptomyces coelicolor (210 aa), FASTA scores: E(): 2.1e-45, (56.8% identity); 9106290|AAF84108.1|AE003963_5|NP_298588.1|NC_002488 protein described as DNA repair system specific for alkylated DNA from Xylella fastidiosa (200 aa), FASTA scores: E(): 3.4e-14, (38.55% identity); and similar in C-terminus to other hypothetical proteins. Note that replaces original Rv1000 predicted on other strand. Q8VK98 No conserved hypotheticals ML0190 MMAR_4493 MSMEG_5451 Mb1027c NC_000962.3 Mycobrowser_v2 CDS 1117185 1118393 . + 0 Rv1001 arcA Arginine degradation [catalytic activity:L-arginine + H(2)O = L-citrulline + NH(3)] Probable arginine deiminase ArcA (adi) (ad) (arginine dihydrolase) Rv1001, (MTCI237.16), len: 402 aa. Probable arcA, arginine deiminase, similar to e.g. ARCA_PSEAE|P13981 arginine deiminase (417 aa), fasta scores: opt: 581, E(): 1.4e-31, (39.4% identity in 411 aa overlap); also similar to SAGP_STRPY|P16962 streptococcal acid glycoprotein (410 aa), FASTA scores, opt: 823, E():0, (38.3% identity in 402 aa overlap). Belongs to the arginine deiminase family. P63551 No intermediary metabolism and respiration ML0191 MMAR_4492 MSMEG_5448 Mb1028 NC_000962.3 Mycobrowser_v2 CDS 1118428 1119939 . - 0 Rv1002c Rv1002c Unknown Conserved membrane protein Rv1002c, (MTCI237.17c), len: 503 aa. Conserved membrane protein. Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). Similar to AL132674|SCE87.05 hypothetical protein from Streptomyces coelicolor (591 aa), FASTA scores: opt: 666, E(): 0, (39.0% identity in 546 aa overlap); weakly similar and to TSCC_PSEAM|P55019 thiazide-sensitive sodium-chloride cotransporter from Pseudopleuronectes americanus (1023 aa), FASTA scores: opt: 44, E(): 4.2e-06, (22.4% identity in 326 aa overlap). O05586 No cell wall and cell processes ML0192 MMAR_4491 MSMEG_5447 Mb1029c NC_000962.3 Mycobrowser_v2 CDS 1120022 1120879 . + 0 Rv1003 Rv1003 Function unknown Conserved protein Rv1003, (MTCI237.19), len: 285 aa. Conserved protein, similar to others e.g. AL132674|SCE87.04 Streptomyces coelicolor (286 aa), FASTA scores: opt: 877, E(): 0, (53.2% identity in 280 aa overlap); and YRAL_ECOLI|P45528 hypothetical 31.3 kd protein (286 aa), FASTA scores: opt: 561, E(): 4.4e-27, (36.9% identity in 279 aa overlap). P0A640 No conserved hypotheticals ML0193 MMAR_4490 MSMEG_5445 Mb1030 NC_000962.3 Mycobrowser_v2 CDS 1120889 1122148 . - 0 Rv1004c Rv1004c Unknown Probable membrane protein Rv1004c, (MTCI237.20c), len: 419 aa. Probable membrane protein. Contains repetitive sequences, which have similarities with elastin, and possible N-terminal signal sequence. O05589 No cell wall and cell processes MMAR_4489 Mb1031c NC_000962.3 Mycobrowser_v2 CDS 1123714 1125417 . + 0 Rv1006 Rv1006 Unknown Unknown protein Rv1006, (MTCI237.23), len: 567 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). O05592 No conserved hypotheticals ML0237 MMAR_4485 Mb1033 NC_000962.3 Mycobrowser_v2 CDS 1125444 1127003 . - 0 Rv1007c metS It is probably essential for cell survival, being required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [catalytic activity: ATP + L-methionine + tRNA(met) = AMP + diphosphate + L-methionyl-tRNA(met)] Methionyl-tRNA synthetase MetS (MetRS) (methionine--tRNA ligase) Rv1007c, (MTCI237.24), len: 519 aa. metS (MetG), methionyl-tRNA synthetase, similar to many e.g. SYM_BACSU|P37465 methionyl-tRNA synthetase from Bacillus subtilus (664 aa), FASTA scores: opt: 1506, E(): 0, (44.9% identity in 492 aa overlap); similar to other Mycobacterium tuberculosis tRNA synthases e.g. Rv2448c, Rv1536, Rv0041. Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Belongs to class-I aminoacyl-tRNA synthetase family. Strong, to cysteinyl-tRNA synthetase. O05593 No information pathways ML0238 MMAR_4481 MSMEG_5441 Mb1034c NC_000962.3 Mycobrowser_v2 CDS 1127089 1127883 . + 0 Rv1008 tatD DNase involved in protein export. This sec-independent pathway is termed tat for twin-arginine translocation system. This system mainly transports proteins with bound cofactors that require folding prior to export. Probable deoxyribonuclease TatD (YJJV protein) Rv1008, (MTCI237.25), len: 264 aa. Probable tatD (alternate gene name: yjjV), deoxyribonuclease, component of twin arginine translocation protein export system (see citations below). Similar to many members of the YBL055C/YJJV family e.g. YCFH_ECOLI|P37346 Putative deoxyribonuclease ycfH (265 aa), fasta scores: opt: 487, E(): 1.4e-24, (36.7% identity in 270 aa overlap). Also similar to P37545|YABD_BACSU Putative deoxyribonuclease yabD (255 aa), FASTA scores: opt: 599, E(): 7.7e-33, (40.1% identity in 262 aa overlap). Contains PS01137 Hypothetical YBL055c/yjjV family signature 1, and PS01091 Hypothetical YBL055c/yjjV family signature 3. O08343 No cell wall and cell processes ML0239 MMAR_4480 MSMEG_5440 Mb1035 NC_000962.3 Mycobrowser_v2 CDS 1128091 1129179 . + 0 Rv1009 rpfB Thought to promote the resuscitation and growth of dormant, nongrowing cells. Could also stimulate the growth of several other high G+C gram+ organisms, e.g. Mycobacterium avium, Mycobacterium bovis (BCG), Mycobacterium kansasii, Mycobacterium smegmatis. Probable resuscitation-promoting factor RpfB Rv1009, (MTCI237.26), len: 362 aa. Probable rpfB, resuscitation-promoting factor (see citation below), similar to others from Mycobacterium tuberculosis: Rv2450c|MTV008.06c|RPFE probable resuscitation-promoting factor (172 aa), FASTA scores: E(): 1.9e-19, (42.9% identity in 147 aa overlap); Rv0867c|RPFA, Rv1884c|RPFC, and Rv2389c|RPFD. Possible lipoprotein; contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Interacts with RipA (see Hett et al., 2007). Predicted possible vaccine candidate (See Zvi et al., 2008). O05594 No cell wall and cell processes ML0240 MMAR_4479 MSMEG_5439 Mb1036 NC_000962.3 Mycobrowser_v2 CDS 1129152 1130105 . + 0 Rv1010 ksgA Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. Its inactivation LEADS to kasugamycin resistance Probable dimethyladenosine transferase KsgA (S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase) (16S rRNA dimethylase) (high level kasugamycin resistance protein KsgA) (kasugamycin dimethyltransferase) Rv1010, (MTCI237.27), len: 317 aa. Probable ksgA, dimethyladenosine transferase, similar to many e.g. KSGA_BACSU|P37468 dimethyladenosine transferase from Bacillus subtilus (292 aa), FASTA scores: opt: 524, E(): 1.5e-28, (37.2% identity in 274 aa overlap); similar to Mycobacterium tuberculosis hypothetical protein Rv1988. Contains PS01131 Ribosomal RNA adenine dimethylases signature. P66660 No information pathways ML0241 MMAR_4478 MSMEG_5438 Mb1037 NC_000962.3 Mycobrowser_v2 CDS 1995054 1995842 . - 0 Rv1762c Rv1762c Unknown Unknown protein Rv1762c, (MTCY28.28c), len: 262 aa. Unknown protein. O06797 No conserved hypotheticals MMAR_2635 Mb1793c NC_000962.3 Mycobrowser_v2 RBS 1995852 1995856 . - 0 Rv1762c Rv1762c Unknown Unknown protein Rv1762c, (MTCY28.28c), len: 262 aa. Unknown protein. O06797 No conserved hypotheticals MMAR_2635 Mb1793c NC_000962.3 Mycobrowser_v2 CDS 1130191 1131111 . + 0 Rv1011 ispE Thought to be involved in deoxyxylulose-5-phosphate pathway (DXP) of isoprenoid biosynthesis (at the fourth step). Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol [catalytic activity: ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol]. Probable 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase IspE (CMK) (4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase) Rv1011, (MTCI237.28, MT1040), len: 306 aa. Probable ispE, 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase , similar to others e.g. Q9K3R6|ISPE_STRCO Streptomyces coelicolor (299 aa), FASTA scores: opt: 925, E(): 2.7e-49, (54.5% identity in 297 overlap); etc. Belongs to the ISPE family. P65178 No intermediary metabolism and respiration ML0242 MMAR_4477 MSMEG_5436 Mb1038 NC_000962.3 Mycobrowser_v2 CDS 1131128 1131421 . + 0 Rv1012 Rv1012 Unknown Hypothetical protein Rv1012, (MTCI237.29), len: 97 aa. Hypothetical unknown protein. O05597 No conserved hypotheticals Mb1039,Mb1040 NC_000962.3 Mycobrowser_v2 CDS 1504356 1505078 . + 0 Rv1337 Rv1337 Unknown Probable integral membrane protein Rv1337, (MTCY130.22), len: 240 aa. Probable integral membrane protein. Highly similar to P53426 hypothetical protein B1549_C3_240 from M.leprae (251); and P74553|D90916 hypothetical protein from Synechocystis sp. (198 aa), FASTA scores: E(): 2.3e-25, (43.6% identity in 181 aa overlap). P64815 No cell wall and cell processes ML1171 MMAR_4059 MSMEG_4904 Mb1372 NC_000962.3 Mycobrowser_v2 CDS 1131625 1133259 . + 0 Rv1013 pks16 Potentially involved in some intermediate steps for the synthesis of a polyketide molecule which may be involved in secondary metabolism. Putative polyketide synthase Pks16 Rv1013, (MTCI237.30-MTCY10G2.36c), len: 544 aa. Putative pks16, polyketide synthase, similar to many e.g. N-terminus of Q50857|U24657 saframycin MX1 synthetase B (1770 aa), FASTA scores: opt: 526, E(): 1.4e-25, (29.3% identity in 542 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family. O05598 No lipid metabolism ML0243 MMAR_4476 MSMEG_5435 Mb1041 NC_000962.3 Mycobrowser_v2 CDS 1133333 1133908 . - 0 Rv1014c pth The natural substrate for this enzyme may be peptidyl-TRNAS which drop off the ribosome during protein synthesis [catalytic activity: N-substituted aminoacyl-tRNA + H(2)O = N-substituted amino acid + tRNA] Probable peptidyl-tRNA hydrolase Pth Rv1014c, (MTCY10G2.35), len: 191 aa. Probable pth, peptidyl-tRNA hydrolase, similar to PTH_ECOLI|P23932 peptidy l-trna hydrolase from Escherichia coli (194 aa), FASTA scores: opt: 472, E(): 2.3e-25, (39.6% identity in 187 aa overlap). Belongs to the PTH family. P65865 No intermediary metabolism and respiration ML0244 MMAR_4473 MSMEG_5432 Mb1042c NC_000962.3 Mycobrowser_v2 CDS 1133921 1134568 . - 0 Rv1015c rplY Binds to the 50S rRNA 50S ribosomal protein L25 RplY Rv1015c, (MTCY10G2.34), len: 215 aa. rplY, 50s ribosomal protein L25, similar to RL25_ECOLI|P02426 50s ribosomal protein L25 from Escherichia coli (94 aa), FASTA scores: opt: 182, E(): 2.5e-05, (38.4% identity in 86 aa overlap) and to CTC_BACSU|P14194 general stress protein from Bacillus subtilis (203 aa), FASTA scores: opt: 260, E(): 1.4e-09, (28.4% identity in 201 aa overlap). Belongs to the L25P family of ribosomal proteins. P66121 No information pathways ML0245 MMAR_4472 MSMEG_5431 Mb1043c NC_000962.3 Mycobrowser_v2 CDS 1168704 1169228 . - 0 Rv1046c Rv1046c Unknown Hypothetical protein Rv1046c, (MTCY10G2.03), len: 174 aa. Hypothetical unknown protein. Start changed since first submission (-65 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O86321 No unknown Mb1075c NC_000962.3 Mycobrowser_v2 CDS 1135501 1136481 . - 0 Rv1017c prsA Catalyzes the formation of PRPP from ATP and ribose 5-phosphate. PRPP is then used in various biosynthetic pathways, as for example in the formation of purines, pyrimidines, histidine and tryptophan. [catalytic activity: ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate] Probable ribose-phosphate pyrophosphokinase PrsA (phosphoribosyl pyrophosphate synthetase) (PRPP synthetase) Rv1017c, (MTCY10G2.32), len: 326 aa. Probable prsA, ribose-phosphate pyrophosphokinase, highly similar to others e.g. KPRS_ECOLI|P08330 ribose-phosphate pyrophosphokinase from Escherichia coli (314 aa), FASTA scores: opt: 826, E(): 0, (43.8% identity in 317 aa overlap). Contains PS00103 Purine/pyrimidine phosphoribosyl transferases signature; contains PS00144 Asparaginase / glutaminase active site signature 1. Belongs to the ribose-phosphate pyrophosphokinase family. Cofactor: both inorganic phosphate and magnesium ion are required for enzyme stability and activity (by similarity). P65232 No intermediary metabolism and respiration ML0248 MMAR_4468 MSMEG_5427 Mb1045c NC_000962.3 Mycobrowser_v2 CDS 1136573 1138060 . - 0 Rv1018c glmU Peptidoglycan and lipopolysaccharide biosynthesis Probable UDP-N-acetylglucosamine pyrophosphorylase GlmU Rv1018c, (MTCY10G2.31), len: 495 aa. Probable glmU, UDP-n-acetylglucosamine pyrophosphorylase, similar to GCAD_BACSU|P14192 UDP-n-acetylglucosamine pyrophosphorylase (456 aa), FASTA scores: opt: 1150, E(): 0, (40.0% identity in 453 aa overlap). Similar to various Mycobacterium tuberculosis sugar-phosphate transferases e.g. Rv0334, Rv1213, Rv3264c, etc. P96382 No cell wall and cell processes ML0249 MMAR_4467 MSMEG_5426 Mb1046c NC_000962.3 Mycobrowser_v2 CDS 1138315 1138908 . + 0 Rv1019 Rv1019 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably TetR-family) Rv1019, (MTCY10G2.30c), len: 197 aa. Probable transcriptional regulator, similar to many memebers of the TetR family e.g. MTCY7D11.18c (34.4% identity in 189 aa overlap). Helix turn helix motif from aa 27-48 (+5.42 SD). P96381 No regulatory proteins ML0250 MSMEG_5424 Mb1047 NC_000962.3 Mycobrowser_v2 CDS 1341006 1341290 . + 0 Rv1198 esxL Unknown Putative ESAT-6 like protein EsxL (ESAT-6 like protein 4) Rv1198, (MT1236, MTCI364.10), len: 94 aa. EsxL, ESAT-6 like protein (see citation below). Member of the ESAT-6 family with Rv3619c, Rv1037c, etc. Almost identical to MTCY10G2.12 (94 aa) (97.9% identity in 94 aa overlap) and MTCY98.022c (94 aa) (94.7% identity in 94 aa overlap). Highly similar to Q49946|U1756D Mycobacterium leprae (95 aa), FASTA scores: opt: 403, E(): 1.1e-22, (64.1% identity in 92 aa overlap). seems to belong to the ESAT6 family. O05300 No cell wall and cell processes ML1056,ML1180 Mb1230 NC_000962.3 Mycobrowser_v2 CDS 1144564 1145853 . + 0 Rv1023 eno Glycolysis [catalytic activity:2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O] Probable enolase Eno Rv1023, (MTCY10G2.26c), len: 429 aa. Probable eno, enolase, highly similar to others e.g. ENO_ECOLI|P08324 enolase from Escherichia coli (431 aa), FASTA scores: opt: 1487, E(): 0, (55.5% identity in 422 aa overlap); etc. Magnesium is required for catalysis and for stabilizing the dimer. Belongs to the enolase family. P96377 No intermediary metabolism and respiration ML0255 MMAR_4462 MSMEG_5415 Mb1051 NC_000962.3 Mycobrowser_v2 CDS 1145858 1146544 . + 0 Rv1024 Rv1024 Unknown Possible conserved membrane protein Rv1024, (MTCY10G2.25c), len: 228 aa. Possible conserved membrane protein, with a hydrophobic region from aa 83-101. Equivalent to ML0256|NP_301311.1|NC_002677 possible conserved membrane protein from Mycobacterium leprae (227 aa), S&W scores: 178, E()= 2e-72, Identities: 145/203 (71%). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P96376 No cell wall and cell processes ML0256 MMAR_4461 Mb1052 NC_000962.3 Mycobrowser_v2 CDS 1147019 1147978 . + 0 Rv1026 Rv1026 Unknown. Could be involved in an adaptive process that allows bacteria to respond to amino acid starvation. Conserved protein Rv1026, (MTCY10G2.23c), len: 319 aa. Conserved protein. Similar to GPPA_ECOLI|P25552 guanosine-5'-triphosphate,3'-diphosphate pyrophoshatase from Escherichia coli (494 aa), FASTA scores: opt: 281, E(): 3.2e-11, (30.6% identity in 291 aa overlap). Equivalent to AL023514|MLCB4.02 hypothetical protein from Mycobacterium leprae (317 aa) (77.9% identity in 321 aa overlap). P96374 No virulence, detoxification, adaptation ML0258 MMAR_4459 MSMEG_5413 Mb1054 NC_000962.3 Mycobrowser_v2 CDS 1148427 1149107 . - 0 Rv1027c kdpE Member of the two-component regulatory system KDPD/KDPE involved in the regulation of the KDP operon. Probable transcriptional regulatory protein KdpE Rv1027c, (MTCY10G2.22), len: 226 aa. Probable KdpE, transcriptional regulatory protein, similar to others e.g. KDPE_ECOLI|P21866 kdp operon transcriptional regulatory protein from Escherichia coli strain K12 (225 aa), FASTA scores: opt: 691, E(): 0, (47.8% identity in 224 aa overlap); AL021530|SC2E9.13 from Streptomyces coelicolor (227 aa), FASTA scores: opt: 981, E(): 0, (66.4% identity in 226 aa overlap); etc. P96373 No regulatory proteins MMAR_0635 MSMEG_5396 Mb1055c NC_000962.3 Mycobrowser_v2 RBS 1172870 1172874 . + 0 Rv1050 Rv1050 Function unknown; probably involved in cellular metabolism Probable oxidoreductase Rv1050, (MTV017.03), len: 301 aa. Probable oxidoreductase similar to many e.g. Rv1543|MTCY48.22C|Q10783 putative oxidoreductase CY48.22C (341 aa), FASTA scores: opt: 462, E(): 3e-22, (33.6% identity in 265 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53398 No intermediary metabolism and respiration Mb1079 NC_000962.3 Mycobrowser_v2 CDS 1172881 1173786 . + 0 Rv1050 Rv1050 Function unknown; probably involved in cellular metabolism Probable oxidoreductase Rv1050, (MTV017.03), len: 301 aa. Probable oxidoreductase similar to many e.g. Rv1543|MTCY48.22C|Q10783 putative oxidoreductase CY48.22C (341 aa), FASTA scores: opt: 462, E(): 3e-22, (33.6% identity in 265 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53398 No intermediary metabolism and respiration Mb1079 NC_000962.3 Mycobrowser_v2 CDS 1149104 1151686 . - 0 Rv1028c kdpD Member of the two-component regulatory system KDPD/KDPE involved in the regulation of the KDP operon. KDPD may function as a membrane-associated protein kinase that phosphorylates KDPE|Rv1027c in response to environmental signals. Probable sensor protein KdpD Rv1028c, (MTCY10G2.21), len: 860 aa. Probable kdpD, sensor protein, similar to others e.g. KDPD_ECOLI|P21865 sensor protein from Escherichia coli strain K12 (894 aa), FASTA scores: opt: 1041, E(): 0, (32.3% identity in 888 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to universal stress protein family. P96372 No regulatory proteins MMAR_0634 MSMEG_5395 Mb1056c NC_000962.3 Mycobrowser_v2 CDS 1151920 1152012 . + 0 Rv1028A kdpF Thought to be involved in stabilization of the KDP complex. Probable membrane protein KdpF Rv1028A, len: 30 aa. Probable kdpF, membrane protein, showing similarity with P36937|KDPF_ECOLI|B0698.1 protein KDPF from Escherichia coli strain K12 (see citation below) (27% identity); and KdpF protein from Streptomyces coelicolor (51% identity). Q79FT7 No cell wall and cell processes Mb1057 NC_000962.3 Mycobrowser_v2 CDS 1165092 1165499 . - 0 Rv1042c Rv1042c Possibly required for the transposition of an insertion element. Probable is like-2 transposase Rv1042c, (MTCY10G2.07), len: 135 aa. Probable is like-2 transposase, similar to Q50761 transposase from Mycobacterium tuberculosis (308 aa), FASTA scores: opt: 823, E(): 0, (99.1% identity in 117 aa overlap). Second copy is Rv1149. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P96359 No insertion seqs and phages Mb1071c NC_000962.3 Mycobrowser_v2 CDS 1152012 1153727 . + 0 Rv1029 kdpA One of the components of the high-affinity ATP-driven potassium transport (or KDP) system, which catalyzes the hydrolysis of ATP coupled with the exchange of hydrogen and potassium ions [catalytic activity: ATP + H(2)O + K(+)(out) = ADP + phosphate + K(+)(in)]. Probable potassium-transporting ATPase a chain KdpA (potassium-translocating ATPase a chain) (ATP phosphohydrolase [potassium-transporting] a chain) (potassium binding and translocating subunit A) Rv1029, (MTCY10G2.20c), len: 571 aa. Probable kdpA, potassium-transporting ATPase a chain (transmembrane protein), similar to others e.g. ATKA_ECOLI|P03959|KDPA|B0698 potassium-transporting ATPase A chain from Escherichia coli strain K12 (557 aa), FASTA