Refseq_ID Mycobrowser_Version Feature Start Stop Score Strand Frame Locus Name Function Product Comments UniProt_AC Is_Pseudogene Functional_Category Protein Data Bank PFAM UniProt Gene Ontology Enzyme Classification Drug Resistance Mutations InterPro UniProtKB/TrEMBL SWISS-MODEL Orthologues M. leprae Orthologues M. marinum Orthologues M. smegmatis Orthologues M. bovis Orthologues M. lepromatosis Orthologues M. tuberculosis Orthologues M. abscessus Orthologues M. haemophilum Orthologues M. orygis NC_000962.3 Mycobrowser_v4 CDS 2719597 2720643 . + 0 Rv2423 Rv2423 Unknown Hypothetical protein Rv2423, (MTCY428.24c), len: 348 aa. Hypothetical unknown protein. P71925 No conserved hypotheticals P71925 P71925 P71925 MMAR_3750 Mb2446 NC_000962.3 Mycobrowser_v4 ncRNA 1413105 1413227 . - MTB000130 ncRv11264c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v4 ncRNA 1455386 1455461 . + MTB000131 ncRv1298 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v4 ncRNA 1455406 1455461 . + MTB000132 ncRv11298 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v4 ncRNA 1473385 1473503 . + MTB000133 ncRv11315 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v4 ncRNA 1497132 1497220 . + MTB000134 ncRv1329 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v4 ncRNA 1564297 1564499 . + MTB000135 ncRv1389 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v4 ncRNA 1692646 1692731 . + MTB000136 ncRv1501 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v4 ncRNA 1816131 1816235 . + MTB000137 ncRv1617 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v4 ncRNA 3837297 3837458 . - MTB000154 ncRv13418cA Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v4 tRNA 2794176 2794249 . + 0 MTB000032 argW - tRNA-Arg (TCT) argW, tRNA-Arg; anticodon tct, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v4 tRNA 2827854 2827926 . + 0 MTB000033 hisT - tRNA-His (GTG) hisT, tRNA-His, anticodon gtg, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v4 CDS 2719083 2719355 . + 0 Rv2422 Rv2422 Unknown Hypothetical protein Rv2422, (MTCY428.25c), len: 90 aa. Hypothetical unknown protein. P71926 No conserved hypotheticals P71926 ML1455 Mb2445 NC_000962.3 Mycobrowser_v4 CDS 2528980 2529174 . - 0 Rv2255c Rv2255c Unknown Hypothetical protein Rv2255c, (MTV022.05c), len: 64 aa. Hypothetical unknown protein. O53529 No conserved hypotheticals O53529 Mb2279c NC_000962.3 Mycobrowser_v4 tRNA 4216865 4216937 . - 0 MTB000048 argU - tRNA-Arg (ACG) argU, tRNA-Arg, anticodon acg, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v4 tRNA 4222581 4222667 . - 0 MTB000050 serU - tRNA-Ser (TGA) serU, tRNA-Ser, anticodon tga, length = 87 No stable RNAs NC_000962.3 Mycobrowser_v4 ncRNA 4314798 4314891 . + 0 MTB000079 ncrMT3949 Unknown Fragment of putative small regulatory RNA ncrMT3949, fragment of putative small regulatory RNA (See Pelly et al., 2012), cloned from M. tuberculosis CDC1551; supported by RNA-seq in H37Rv (unpublished data). No stable RNAs NC_000962.3 Mycobrowser_v4 CDS 5240 7267 . + 0 Rv0005 gyrB DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [catalytic activity: ATP-dependent breakage, passage and rejoining of double-stranded DNA]. DNA gyrase (subunit B) GyrB (DNA topoisomerase (ATP-hydrolysing)) (DNA topoisomerase II) (type II DNA topoisomerase) Rv0005, (MTCY10H4.03), len: 675 aa. GyrB, DNA gyrase subunit B (see citations below). Contains PS00177 DNA topoisomerase II signature. Belongs to the type II topoisomerase family. Start changed since first submission (-39 aa). P9WG45 No information pathways 2ZJT,3IG0,3M4I P0C5C5 P9WG45 GO:0003918,GO:0006265,GO:0005524 5.99.1.3 FLQ P9WG45 ML0005 MMAR_0005 MSMEG_0005 Mb0005 NC_000962.3 Mycobrowser_v4 CDS 39056 39829 . - 0 Rv0036c Rv0036c Function unknown Conserved protein Rv0036c, (MTCY10H4.36c), len: 257 aa. Conserved protein, highly similar to CAB95889.1|AL359988 conserved hypothetical protein from Streptomyces (276 aa). Also some similarity to Rv3099c|MTCY164_10 (283 aa), FASTA scores: E(): 3.3e-05, (25.9% identity in 205 aa overlap). P9WM91 No conserved hypotheticals P64675 P9WM91 P9WM91 MMAR_0051 MSMEG_6923 Mb0037c NC_000962.3 Mycobrowser_v4 CDS 165323 165826 . + 0 Rv0138 Rv0138 Function unknown Conserved hypothetical protein Rv0138, (MTCI5.12), len: 167 aa. Conserved hypothetical protein, showing weak similarity to Q10827|YT10_MYCTU hypothetical 17.0 KDA protein from Mycobacterium tuberculosis (147 aa), FASTA scores: opt: 131, E(): 0.047, (31.15% identity in 106 aa overlap). P96815 No conserved hypotheticals P96815 P96815 ML2646,ML2646c MMAR_0348 MSMEG_6475 Mb0143 NC_000962.3 Mycobrowser_v4 CDS 2386293 2387171 . + 0 Rv2125 Rv2125 Unknown Conserved hypothetical protein Rv2125, (MTCY261.21), len: 292 aa. Conserved hypothetical protein, similar to many. O33260 No conserved hypotheticals O33260 O33260 O33260 ML1306,ML1306c MMAR_3107 MSMEG_4186 Mb2149 NC_000962.3 Mycobrowser_v4 CDS 4140239 4140463 . - 0 Rv3697A vapB48 Unknown Possible antitoxin VapB48 Rv3697A, len: 74 aa. Possible vapB48, antitoxin, part of toxin-antitoxin (TA) operon with Rv3697c, see Arcus et al. 2005. Similar to others in M. tuberculosis e.g. Rv3321c, Rv0748 P9WJ15 No virulence, detoxification, adaptation P9WJ15 P9WJ15 NC_000962.3 Mycobrowser_v4 CDS 4305757 4306239 . + 0 Rv3831 Rv3831 Unknown Hypothetical protein Rv3831, (MTCY01A6.38c), len: 160 aa. Hypothetical unknown protein. P96247 No conserved hypotheticals P96247 P96247 ML0083,ML0083c MMAR_5385 Mb3861 NC_000962.3 Mycobrowser_v4 CDS 4324683 4324967 . + 0 Rv3851 Rv3851 Unknown Possible membrane protein Rv3851, (MTCY01A6.17c), len: 94 aa. Possible membrane protein. P96226 No cell wall and cell processes P96226 P96226 Mb3881 NC_000962.3 Mycobrowser_v4 tRNA 25644 25726 . + 0 MTB000003 leuT - tRNA-Leu (CAG) leuT, tRNA-Leu, anticodon cag, length = 83 No stable RNAs NC_000962.3 Mycobrowser_v4 CDS 14914 15612 . + 0 Rv0013 trpG Possibly involved in biosynthesis of tryptophan (at the first step). Supposed tetramer of two components I and two components II: component I (Rv1609|trpE) catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II (Rv0013|trpG) provides glutamine amidotransferase activity. Possibly participates in the tryptophan-dependent indole-3-acetic acid production [catalytic activity: chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate]. Possible anthranilate synthase component II TrpG (glutamine amidotransferase) Rv0013, (MTCY10H4.13), len: 232 aa. Possible trpG, anthranilate synthase component II (glutamine amidotransferase). Contains PS00606 Beta-ketoacyl synthases active site; and PS00442 Glutamine amidotransferases class-I active site. Similarity to other type-1 glutamine amidotransferase domains. Note that previously known as pabA. P9WN35 No intermediary metabolism and respiration Q7DAK6 P9WN35 GO:0006541,GO:0000162,GO:0004049 4.1.3.27 P9WN35 ML0015 MMAR_0015 MSMEG_0029 Mb0013 NC_000962.3 Mycobrowser_v4 CDS 17467 18762 . - 0 Rv0015c pknA Involved in signal transduction (via phosphorylation). Thought to regulate morphological changes associated with cell division/differentiation process. Phosphorylates at serine and threonine residues [catalytic activity: ATP + a protein = ADP + a phosphoprotein]. Transmembrane serine/threonine-protein kinase A PknA (protein kinase A) (STPK A) Rv0015c, (MTCY10H4.15c), len: 431 aa. PknA, transmembrane serine/threonine-protein kinase, magnesium/manganese dependent (see citations below). Contains PS00108 Serine/Threonine protein kinases active-site signature. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases. It has been shown that sodium orthovanadate inhibits the activity of the enzyme in vitro. P9WI83 No regulatory proteins P65726 P9WI83 GO:0006468,GO:0004674,GO:0005524 2.7.11.1 P9WI83 ML0017,ML0017c MMAR_0017 MSMEG_0030 Mb0015c NC_000962.3 Mycobrowser_v4 CDS 23861 25444 . - 0 Rv0020c fhaA Signal transduction Conserved protein with FHA domain, FhaA Rv0020c, (MTCY10H4.20c), len: 527 aa. FhaA, TB39.8, conserved protein with forkhead-associated domain (IPR000253) at C-terminus, may be involved in signal transduction. Alternative start codon in position 24979 has been suggested (see citation below). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P71590 No regulatory proteins 3PO8,3POA,2LC0,2LC1 P71590 P71590 P71590 ML0022,ML0022c MMAR_0022 MSMEG_0035 Mb0020c NC_000962.3 Mycobrowser_v4 CDS 2322552 2323178 . + 0 Rv2065 cobH Required for cobalamin biosynthesis. Precorrin-8X methylmutase CobH (aka precorrin isomerase) Rv2065, (MTCY49.04), len: 208 aa. CobH, precorrin-8X methylmutase (aka precorrin isomerase), similar to many. P9WP87 No intermediary metabolism and respiration P63839 P9WP87 GO:0016993,GO:0009236 5.4.1.2 P9WP87 MMAR_3045 MSMEG_3872 Mb2091 NC_000962.3 Mycobrowser_v4 CDS 20231 21640 . - 0 Rv0017c rodA This is a septum-peptidoglycan biosynthetic protein, involved in cell wall formation. Plays a role in the stabilization of the FTSZ ring during cell division. Probable cell division protein RodA Rv0017c, (MTCY10H4.17c), len: 469 aa. Probable rodA (alternate gene name: ftsW), cell division protein, integral membrane protein. Belongs to the FTSW/RODA/SPOVE family. P9WN99 No cell wall and cell processes P63760 P9WN99 GO:0051301,GO:0007047,GO:0016021,GO:0009252,GO:0005886,GO:0008360,GO:0007049 ML0019,ML0019c MMAR_0019 MSMEG_0032 Mb0017c NC_000962.3 Mycobrowser_v4 CDS 23270 23737 . - 0 Rv0019c fhaB Signal transduction Conserved protein with FHA domain, FhaB Rv0019c, (MTCY10H4.19c), len: 155 aa. FhaB, conserved protein with forkhead-associated domain (IPR000253), probably involved in signal transduction. P9WJB5 No regulatory proteins P71589 P9WJB5 P9WJB5 ML0021,ML0021c MMAR_0021 MSMEG_0034 Mb0019c NC_000962.3 Mycobrowser_v4 CDS 27023 27442 . - 0 Rv0022c whiB5 Involved in transcriptional mechanism. Probable transcriptional regulatory protein WhiB-like WhiB5 Rv0022c, (MTCY10H4.22c), len: 139 aa. Probable whiB5 (alternate gene name: whmG), WhiB-like regulatory protein (see citations below), similar to WhiB paralogue of Streptomyces coelicolor, wblE gene product (85 aa). P71592 No regulatory proteins P71592 P71592 P71592 MMAR_0041 Mb0022c NC_000962.3 Mycobrowser_v4 CDS 37259 38947 . + 0 Rv0035 fadD34 Function unknown, but involved in lipid degradation. Probable fatty-acid-CoA ligase FadD34 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv0035, (MTCY10H4.35), len: 562 aa. Probable fadD34, fatty-acid-CoA synthetase, similar to many. L7N699 No lipid metabolism Q7DAJ8 L7N699 GO:0008152,GO:0016874 6.2.1.- L7N699 MMAR_4970 Mb0036 NC_000962.3 Mycobrowser_v4 CDS 59122 59376 . + 0 Rv0055 rpsR1 This protein has been implicated in aminoacyl-transfer RNA binding. It appears to be situated at the decoding site of messenger RNA. 30S ribosomal protein S18-1 RpsR1 Rv0055, (MTCY21D4.18), len: 84 aa. rpsR1, 30S ribosomal protein S18-1. Belongs to the S18P family of ribosomal proteins. Note that previously known as rpsR. P9WH49 No information pathways P69230 P9WH49 GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WH49 ML2683,ML2683c MMAR_0074 MSMEG_6895 Mb0056 NC_000962.3 Mycobrowser_v4 CDS 1175723 1176112 . + 0 Rv1052 Rv1052 Unknown Hypothetical protein Rv1052, (MTV017.05), len: 129 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53400 No conserved hypotheticals O53400 Mb1081 NC_000962.3 Mycobrowser_v4 CDS 3051619 3051792 . + 0 Rv2737A Rv2737A Function unknown Conserved hypothetical cysteine rich protein (fragment) Rv2737A, len: 57 aa. Conserved hypothetical cys-rich protein (possibly gene fragment), similar to central part of AJ243803_1|glgA from Streptomyces coelicolor glgA (181 aa), FASTA scores: opt: 210, E(): 6.1e-09, (59.25% identity in 54 aa overlap). Q79FB4 No conserved hypotheticals Q79FB4 Mb2757 NC_000962.3 Mycobrowser_v4 CDS 46581 47207 . - 0 Rv0042c Rv0042c Possibly involved in transcriptional mechanism. Possible transcriptional regulatory protein (probably MarR-family) Rv0042c, (MTCY21D4.05c), len: 208 aa. Possible transcriptional regulatory protein, MarR-family. Some similarity to Mycobacterium tuberculosis proteins Rv2327, Rv0880, and Rv1404. P71699 No regulatory proteins P71699 P71699 GO:0006355,GO:0006350,GO:0003700,GO:0005622 P71699 ML2696 MMAR_0059 MSMEG_6913 Mb0043c NC_000962.3 Mycobrowser_v4 CDS 47366 48100 . - 0 Rv0043c Rv0043c Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably GntR-family) Rv0043c, (MTCY21D4.06c), len: 244 aa. Probable transcriptional regulator, GntR family, similar to others. P9WMG9 No regulatory proteins P67737 P9WMG9 GO:0006355,GO:0006350,GO:0003700,GO:0005622 P9WMG9 MMAR_0062 MSMEG_6908 Mb0044c NC_000962.3 Mycobrowser_v4 CDS 48233 49027 . - 0 Rv0044c Rv0044c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0044c, (MTCY21D4.07c), len: 264 aa. Possible oxidoreductase, highly similar to AAD32732.1|MmcI|AF127374| F420-dependent H4MPT reductase from Streptomyces lavendulae (264 aa). Also similar to Mycobacterium tuberculosis proteins e.g. Rv1855c, Rv0953c, Rv0791c, Rv0132c, etc. P71701 No intermediary metabolism and respiration P71701 P71701 GO:0016491,GO:0055114 1.-.-.- P71701 ML2694 MMAR_0063 MSMEG_6907 Mb0045c NC_000962.3 Mycobrowser_v4 CDS 49043 49939 . - 0 Rv0045c Rv0045c Function unknown; probably involved in lipid biosynthesis. Possible hydrolase Rv0045c, (MTCY21D4.08c), len: 298 aa. Possible hydrolase, showing similarity with others. Also similar to Mycobacterium tuberculosis proteins Rv3473c, Rv1123c, Rv1938, Rv3617, Rv3670, etc. I6XU97 No lipid metabolism 3P2M P71702 I6XU97 GO:0016787 3.-.-.- I6XU97 ML2693 MMAR_0064 MSMEG_6906 Mb0046c NC_000962.3 Mycobrowser_v4 CDS 50021 51124 . - 0 Rv0046c ino1 Involved in phosphatidylinositol (PI) biosynthetic pathway [catalytic activity: D-glucose 6-phosphate = 1L-myo-inositol 1-phosphate]. myo-inositol-1-phosphate synthase Ino1 (inositol 1-phosphate synthetase) (D-glucose 6-phosphate cycloaldolase) (glucose 6-phosphate cyclase) (glucocycloaldolase) Rv0046c, (MTCY21D4.09c), len: 367 aa. Ino1 (alternate gene name: tbINO), myo-inositol-1-phosphate synthase (see citations below). P9WKI1 No intermediary metabolism and respiration 1GR0 P71703 P9WKI1 GO:0006021,GO:0004512,GO:0008654 P9WKI1 ML2692,ML0396c MMAR_0065 MSMEG_6904 Mb0047c NC_000962.3 Mycobrowser_v4 CDS 349624 349932 . + 0 Rv0285 PE5 Function unknown PE family protein PE5 Rv0285, (MTV035.13), len: 102 aa. PE5, Member of the Mycobacterium tuberculosis PE family (see Brennan & Delogu 2002), similar to others e.g. AL0212|MTV012_37 from Mycobacterium tuberculosis (105 aa), FASTA scores: opt: 497, E(): 2.6e-24, (80.4% identity in 102 aa overlap); Z80108|MTCY21B4.03 from Mycobacterium tuberculosis (102 aa), FASTA scores: opt: 413, E(): 3.7e-19, (66.7% identity in 102 aa overlap); etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). L7N695 No PE/PPE Q7DA36 L7N695 L7N695 ML2534,ML2534c MMAR_0544 MSMEG_0618 Mb0293 NC_000962.3 Mycobrowser_v4 CDS 55696 57378 . + 0 Rv0051 Rv0051 Unknown Probable conserved transmembrane protein Rv0051, (MTCY21D4.14), len:560 aa. Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). Probable conserved transmembrane protein. P71708 No cell wall and cell processes P71708 P71708 GO:0016021 ML2687,ML2687c MMAR_0070 MSMEG_6899 Mb0052 NC_000962.3 Mycobrowser_v4 CDS 57410 57973 . + 0 Rv0052 Rv0052 Function unknown Conserved protein Rv0052, (MTCY21D4.15), len: 187 aa. Conserved protein, similar to others including Rv1930c from Mycobacterium tuberculosis (174 aa). May be a membrane protein. I6Y6S3 No conserved hypotheticals P71709 I6Y6S3 I6Y6S3 ML2686,ML2686c MMAR_0071 Mb0053 NC_000962.3 Mycobrowser_v4 CDS 58586 59080 . + 0 Rv0054 ssb This protein is essential for replication of the chromosome. It is also involved in DNA recombination and repair. Single-strand binding protein Ssb (helix-destabilizing protein) Rv0054, (MTCY21D4.17), len: 164 aa. ssb, single-strand binding protein (see Mizrahi & Andersen 1998), highly similar to others. Belongs to the SSB family. P9WGD5 No information pathways 1UE1,1UE5,1UE6,1UE7 P0A610 P9WGD5 GO:0006260,GO:0003697,GO:0006281 P9WGD5 ML2684,ML2684c MMAR_0073 MSMEG_6896 Mb0055 NC_000962.3 Mycobrowser_v4 CDS 59409 59867 . + 0 Rv0056 rplI Binds to the 23S rRNA. 50S ribosomal protein L9 RplI Rv0056, (MTCY21D4.19), len: 152 aa. rplI, 50S ribosomal protein L9. Contains PS00651 Ribosomal protein L9 signature. Belongs to the L9P family of ribosomal proteins. P9WH79 No information pathways P66315 P9WH79 GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WH79 ML2682,ML2682c MMAR_0075 MSMEG_6894 Mb0057 NC_000962.3 Mycobrowser_v4 CDS 59896 60417 . + 0 Rv0057 Rv0057 Unknown Hypothetical protein Rv0057, (MTCY21D4.20), len: 173 aa. Hypothetical unknown protein. P9WM77 No conserved hypotheticals P9WM77 ML2681,ML2681c Mb0058 NC_000962.3 Mycobrowser_v4 CDS 60396 63020 . + 0 Rv0058 dnaB Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity. The intein is an endonuclease (potential). Probable replicative DNA helicase DnaB Rv0058, (MTV030.01, MTCY21D4.21), len: 874 aa. Probable dnaB, replicative DNA helicase. Contains an intein (position 61630..62838) similar to, and in the same position as, those in Sycnechocystis and Rhodothermus marinus (see citation below) and C-terminal extein (position 62839..63015) similar to many dnaB proteins. This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation. Belongs to the helicase family, DNAB subfamily. In the intein section; belongs to the homing endonuclease family. P9WMR3 No information pathways 2R5U P71715 P9WMR3 GO:0003677,GO:0003678,GO:0006269,GO:0005658,GO:0004519,GO:0016539,GO:0006314,GO:0005524 3.1.-.-,3.6.4.12 P9WMR3 ML2680,ML2680c MSMEG_6892 Mb0059 NC_000962.3 Mycobrowser_v4 CDS 63200 63892 . + 0 Rv0059 Rv0059 Unknown Hypothetical protein Rv0059, (MTV030.02), len: 230 aa. Hypothetical unknown protein. O53604 No conserved hypotheticals O53604 Mb0060 NC_000962.3 Mycobrowser_v4 CDS 74629 75198 . - 0 Rv0067c Rv0067c Possibly involved in transcriptional mechanism. Possible transcriptional regulatory protein (possibly TetR-family) Rv0067c, (MTV030.10c), len: 189 aa. Possible transcriptional regulator, highly similar to many. Contains probable helix-turn-helix motif from aa 34 to 55 (Score 1523, +4.37 SD). O53612 No regulatory proteins O53612 O53612 GO:0006350,GO:0003700,GO:0006355 O53612 ML0316 MSMEG_0878 Mb0068c NC_000962.3 Mycobrowser_v4 rRNA 1473658 1476795 . + 0 MTB000020 rrl - Ribosomal RNA 23S rrl, 23S rRNA gene (approximate coordinates). No stable RNAs NC_000962.3 Mycobrowser_v4 CDS 66923 68362 . + 0 Rv0063 Rv0063 Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0063, (MTV030.06), len: 479 aa. Possible oxidoreductase, similar to many. Similar to Mycobacterium tuberculosis proteins e.g. Rv3107c, Rv1257c, etc. Contains PS00862 Oxygen oxidoreductases covalent FAD-binding site. O53608 No intermediary metabolism and respiration O53608 O53608 GO:0055114,GO:0016491,GO:0050660 1.-.-.- O53608 MMAR_0110 Mb0064 NC_000962.3 Mycobrowser_v4 CDS 68620 71559 . + 0 Rv0064 Rv0064 Unknown Probable conserved transmembrane protein Rv0064, (MTV030.07), len: 979 aa. Probable conserved transmembrane protein, similar to many. Contains probable coiled-coil domain from aa 948 to 976. P9WFL5 No cell wall and cell processes O53609 P9WFL5 GO:0005886,GO:0016021 Mb0065 NC_000962.3 Mycobrowser_v4 CDS 71589 71828 . + 0 Rv0064A vapB1 Unknown Possible antitoxin VapB1 Rv0064A, len: 79 aa. Possible vapB1, antitoxin, part of toxin-antitoxin (TA) operon with Rv0065 (See Arcus et al., 2005; Pandey and Gerdes, 2005). Weakly similar to others in Mycobacterium tuberculosis e.g. Rv0300 (73 aa), Rv1721c (75 aa) P0CW29 No virulence, detoxification, adaptation P0CW29 NC_000962.3 Mycobrowser_v4 CDS 71821 72222 . + 0 Rv0065 vapC1 Unknown Possible toxin VapC1 Rv0065, (MTV030.08), len: 133 aa. Possible vapC1, toxin, part of toxin-antitoxin (TA) operon with Rv0064A, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to several others in Mycobacterium tuberculosis: Rv0960 (127 aa), Rv1720c (129 aa), and Rv0549c (137 aa). P9WFC1 No virulence, detoxification, adaptation P9WFC1 P9WFC1 Mb0066 NC_000962.3 Mycobrowser_v4 CDS 89575 89919 . + 0 Rv0081 Rv0081 Involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv0081, (MTV030.25), len: 114 aa. Probable transcriptional regulator, highly similar to others. P9WMI7 No regulatory proteins O53626 P9WMI7 GO:0006355,GO:0006350,GO:0003700,GO:0005622 P9WMI7 MMAR_1653 MSMEG_6451 Mb0084 NC_000962.3 Mycobrowser_v4 CDS 89924 90403 . + 0 Rv0082 Rv0082 Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv0082, (MTV030.26), len: 159 aa. Probable oxidoreductase, highly similar or similar to other various oxidoreductases. Nucleotide position 90144 in the genome sequence has been corrected, A:G resulting in Q74R. I6XUD2 No intermediary metabolism and respiration O53627 I6XUD2 GO:0008137,GO:0055114,GO:0048038,GO:0051539 1.-.-.- I6XUD2 MMAR_1654 Mb0085 NC_000962.3 Mycobrowser_v4 CDS 2073943 2074437 . + 0 Rv1829 Rv1829 Function unknown Conserved protein Rv1829, (MTCY1A11.14c), len: 164 aa. Conserved protein, equivalent to O32917|MLCB1788.34|AL008609 Hypothetical protein from Mycobacterium leprae (164 aa), FASTA scores: opt: 1011, E(): 0, (95.1% identity in 164 aa overlap). Also present in Aquifex aeolicus, etc. P9WLR5 No conserved hypotheticals Q50604 P9WLR5 P9WLR5 ML2074,ML2074c MMAR_2706 MSMEG_3645 Mb1860 NC_000962.3 Mycobrowser_v4 CDS 77619 78896 . - 0 Rv0070c glyA2 Key enzyme in the biosynthesis of purines, lipids, other components. Interconversion of serine and glycine [catalytic activity: 5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine]. Serine hydroxymethyltransferase GlyA2 (serine methylase 2) (SHMT 2) Rv0070c, (MTV030.13c), len: 425 aa. glyA2, serine hydroxymethyltransferase. Contains PS00096 Serine hydroxymethyltransferase pyridoxal-phosphate attachment site. Belongs to the ShmT family. Cofactor: pyridoxal phosphate. P9WGI7 No intermediary metabolism and respiration O53615 P9WGI7 GO:0004372,GO:0006544,GO:0006730,GO:0030170,GO:0006563 2.1.2.1 P9WGI7 MMAR_0208 Mb0071c NC_000962.3 Mycobrowser_v4 CDS 79486 80193 . + 0 Rv0071 Rv0071 Unknown Possible maturase Rv0071, (MTV030.14), len: 235 aa. Possible maturase, similar to many proteins of the group II intron maturase family. Contains 5 VDP repeats at N-terminus, these are also found in two Streptococcus plasmid hypothetical proteins Q52246|X17092 and Q54942|X66468. O53616 No information pathways O53616 O53616 GO:0006278,GO:0003964,GO:0003723 O53616 Mb0072 NC_000962.3 Mycobrowser_v4 CDS 80624 81673 . + 0 Rv0072 Rv0072 Thought to be involved in active transport of glutamine across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable glutamine-transport transmembrane protein ABC transporter Rv0072, (MTV030.16), len: 349 aa. Probable glutamine-transport transmembrane protein ABC-transporter (see citation below). Note that supposed act with near ORF Rv0073|MTV030.17 ATP-binding protein ABC-transporter. P9WG17 No cell wall and cell processes O53617 P9WG17 GO:0005886,GO:0016021 P9WG17 MMAR_0215 Mb0073 NC_000962.3 Mycobrowser_v4 CDS 82748 83983 . + 0 Rv0074 Rv0074 Function unknown Conserved protein Rv0074, (MTV030.18), len: 411 aa. Conserved protein, similar to many. O53619 No conserved hypotheticals O53619 O53619 GO:0016810 O53619 ML1110 MMAR_4746 MSMEG_0297,MSMEG_4842 Mb0076 NC_000962.3 Mycobrowser_v4 CDS 83996 85168 . + 0 Rv0075 Rv0075 Function unknown; probably involved in cellular metabolism. Probable aminotransferase Rv0075, (MTV030.19), len: 390 aa. Probable aminotransferase, similar to many class-II pyridoxal-phosphate-dependent aminotransferases (MALY/PATB subfamily). Also similar to other proteins from Mycobacterium tuberculosis e.g. Rv2294, Rv0858c, etc. O53620 No intermediary metabolism and respiration O53620 O53620 GO:0030170,GO:0008483,GO:0009058 2.6.1.- O53620 ML1108,ML1108c Mb0077 NC_000962.3 Mycobrowser_v4 CDS 85183 85572 . - 0 Rv0076c Rv0076c Unknown Probable membrane protein Rv0076c, (MTV030.20c), len: 129 aa. Probable membrane protein, with membrane-spanning domain at C-terminus. O53621 No cell wall and cell processes O53621 MMAR_0219 MSMEG_0583 Mb0078c NC_000962.3 Mycobrowser_v4 CDS 167271 167681 . - 0 Rv0141c Rv0141c Unknown Unknown protein Rv0141c, (MTCI5.15c), len: 136 aa. Unknown protein. P96818 No conserved hypotheticals P96818 P96818 ML2644 MMAR_0351 Mb0146c NC_000962.3 Mycobrowser_v4 CDS 608059 608535 . - 0 Rv0516c Rv0516c May be involved in regulating sigma factor Possible anti-anti-sigma factor Rv0516c, (MTCY20G10.06c), len: 158 aa. Possible anti-anti-sigma factor, showing some similarity to Rv1365c|MTCY02B10_29 from Mycobacterium tuberculosis (128 aa), FASTA scores: E(): 0.0012, (27.4% identity in 124 aa overlap). O33361 No information pathways O33361 O33361 O33361 MSMEG_0586 Mb0529c NC_000962.3 Mycobrowser_v4 CDS 92328 93278 . + 0 Rv0084 hycD Involved in hydrogen metabolism; FHL pathway. Possible formate hydrogenlyase HycD (FHL) Rv0084, (MTCY251.02), len: 316 aa. Possible hycD (alternate gene name: hevD), formate hydrogenlyase, integral membrane protein, similar to others. Belongs to the complex I subunit 1 family. Q10881 No intermediary metabolism and respiration Q10881 Q10881 GO:0016829,GO:0055114,GO:0016491,GO:0016021 1.-.-.- Q10881 MMAR_1656 Mb0087 NC_000962.3 Mycobrowser_v4 CDS 93289 93951 . + 0 Rv0085 hycP Involved in hydrogen metabolism. Possible hydrogenase HycP Rv0085, (MTCY251.03), len: 220 aa. Possible hycP, hydrogenase, integral membrane protein. Belongs to NADH-ubiquinone/plastoquinone oxidoreductase chain 4L superfamily. P9WM75 No intermediary metabolism and respiration P9WM75 GO:0005886,GO:0016021 MMAR_1657 Mb0088 NC_000962.3 Mycobrowser_v4 CDS 93951 95417 . + 0 Rv0086 hycQ Possibly involved in hydrogen metabolism. Possible hydrogenase HycQ Rv0086, (MTCY251.04), len: 488 aa. Possible hycQ, hydrogenase, integral membrane protein. Belongs to the NADH-Ubiquinone/plastoquinone (complex I) superfamily. Q10883 No intermediary metabolism and respiration Q10883 Q10883 GO:0008137,GO:0016021,GO:0042773 1.-.-.- MMAR_1658 Mb0089 NC_000962.3 Mycobrowser_v4 CDS 95414 96892 . + 0 Rv0087 hycE Involved in hydrogen metabolism; FHL pathway. Possible formate hydrogenase HycE (FHL) Rv0087, (MTCY251.05), len: 492 aa. Possible hycE (alternate gene name: hevE), formate hydrogenlyase, similar to others. Belongs to the complex I 49 kDa subunit family. Q10884 No intermediary metabolism and respiration Q10884 Q10884 GO:0008137,GO:0008901,GO:0016151,GO:0055114,GO:0048038,GO:0051287 1.-.-.- Q10884 MMAR_1659 Mb0090 NC_000962.3 Mycobrowser_v4 CDS 96927 97601 . + 0 Rv0088 Rv0088 Unknown Possible polyketide cyclase/dehydrase Rv0088, (MTCY251.06), len: 224 aa. Possible polyketide cyclase/dehydrase. Belongs to the SRPBCC ligand-binding domain superfamily. Predicted to be an outer membrane protein (See Song et al., 2008). P9WM73 No lipid metabolism P0A5C3 P9WM73 P9WM73 ML2652 MMAR_0234 Mb0091 NC_000962.3 Mycobrowser_v4 CDS 97758 98351 . + 0 Rv0089 Rv0089 Thought to cause methylation. Possible methyltransferase/methylase Rv0089, (MTCY251.07), len: 197 aa. Possible methyltransferase, showing some weak similarity to others. Also some similarity with many biotin biosynthesis proteins. Belongs to the methyltransferase superfamily. P9WK03 No intermediary metabolism and respiration P65346 P9WK03 GO:0008168,GO:0008152 2.1.1.- P9WK03 MMAR_0244 MSMEG_4708 Mb0092 NC_000962.3 Mycobrowser_v4 CDS 329705 330325 . - 0 Rv0273c Rv0273c Could be involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0273c, (MTV035.01c), len: 206 aa (start uncertain). Possible transcriptional regulator, showing some similarity to hypothetical regulators from Mycobacterium tuberculosis e.g. P96222|Rv3855|MTCY01A6.13c (216 aa); O08377|Rv1534|MTCY07A7A.03 (225 aa), FASTA scores: opt: 123, E(): 3.2e-06, (28.5% identity in 172 aa overlap). O86342 No regulatory proteins O86342 O86342 GO:0006350,GO:0003700,GO:0006355 O86342 ML2543 MMAR_0533 MSMEG_0606 Mb0279c NC_000962.3 Mycobrowser_v4 CDS 1183508 1183981 . + 0 Rv1060 Rv1060 Unknown Unknown protein Rv1060, (MTV017.13), len: 157 aa. Unknown protein. O53408 No conserved hypotheticals O53408 O53408 O53408 MMAR_4406 Mb1089 NC_000962.3 Mycobrowser_v4 CDS 102815 103663 . - 0 Rv0093c Rv0093c Unknown Probable conserved membrane protein Rv0093c, (MTCY251.12c), len: 282 aa. Probable conserved membrane protein, equivalent only to CAC30943.1|AL583924 probable integral membrane protein from Mycobacterium leprae (237 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WM69 No cell wall and cell processes P9WM69 GO:0005886,GO:0016021 ML1988,ML1988c MMAR_0263 Mb0096c NC_000962.3 Mycobrowser_v4 CDS 103710 104663 . - 0 Rv0094c Rv0094c Function unknown Conserved hypothetical protein Rv0094c, (MTCY251.13c), len: 317 aa. Member of 13E12 repeat family, showing some similarity to U15187|MLU15187_7 from Mycobacterium leprae (94 aa), FASTA score: (49.4% identity in 79 aa overlap). Q50655 No insertion seqs and phages Q50655 Q50655 Mb0097c NC_000962.3 Mycobrowser_v4 CDS 104805 105215 . - 0 Rv0095c Rv0095c Function unknown Conserved hypothetical protein Rv0095c, (MTCY251.14c), len: 136 aa. Member of 13E12 repeat, also partially similar to AF0418|AF041819_8 from Mycobacterium bovis BCG (222 aa), FASTA score: (89.6% identity in 96 aa overlap). Q10891 No insertion seqs and phages Q10891 Mb0098c NC_000962.3 Mycobrowser_v4 CDS 106734 107603 . + 0 Rv0097 Rv0097 Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0097, (MTCY251.16), len: 289 aa. Possible oxidoreductase, equivalent to NP_302343.1|NC_002677 putative oxidoreductase from Mycobacterium leprae (289 aa). Also highly similar to BAB69377.1|AB070955 putative oxidoreductase from Streptomyces avermitilis (296 aa). Contains PS00077 Cytochrome c oxidase subunit I, copper B binding region signature. P9WG83 No intermediary metabolism and respiration P67755 P9WG83 GO:0055114,GO:0016702,GO:0046872 1.-.-.- P9WG83 ML1992 MMAR_0260 Mb0100 NC_000962.3 Mycobrowser_v4 CDS 107600 108151 . + 0 Rv0098 fcoT Involved in fatty acid metabolism Probable fatty acyl CoA thioesterase type III FcoT Rv0098, (MTCY251.17), len: 183 aa. FcoT, long-chain fatty acyl CoA thioesterase type III (See Wang et al., 2007), equivalent to CAC30948.1|AL583924 from Mycobacterium leprae (183 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WM67 No lipid metabolism 2PFC P64685 P9WM67 P9WM67 ML1993 MMAR_0259 Mb0101 NC_000962.3 Mycobrowser_v4 CDS 109783 110019 . + 0 Rv0100 Rv0100 Function unknown Conserved hypothetical protein Rv0100, (MTCY251.19), len: 78 aa. Conserved hypothetical protein, equivalent only to CAC30950.1|AL583924 conserved hypothetical protein from Mycobacterium leprae (78 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WM65 No conserved hypotheticals P64687 P9WM65 GO:0048037 ML1995 MMAR_0257 Mb0103 NC_000962.3 Mycobrowser_v4 CDS 591475 591576 . + 0 Rv0500B Rv0500B Function unknown Conserved hypothetical protein Rv0500B, len: 33 aa. Conserved hypothetical protein. Basic protein 18 of the 33 aa are Arg or Lys, with strong similarity to AL079345|SCE68_25 protein from Streptomyces coelicolor cosmid E6 (32 aa), FASTA scores: opt: 176, E(): 1e-06, (93.1% identity in 29 aa overlap). Same gene arrangement in both actinomycetes. P9WKT5 No conserved hypotheticals P0A5C9 P9WKT5 P9WKT5 ML2428A NC_000962.3 Mycobrowser_v4 CDS 884072 884800 . - 0 Rv0790c Rv0790c Unknown Hypothetical protein Rv0790c, (MTCY369.34c), len: 242 aa. Hypothetical unknown protein. I6XWA9 No conserved hypotheticals P71843 I6XWA9 I6XWA9 ML2217 MMAR_2558 Mb0814c NC_000962.3 Mycobrowser_v4 CDS 119915 122173 . - 0 Rv0103c ctpB Cation-transporting ATPase; possibly catalyzes the transport of a cation (possibly coopper) with the hydrolyse of ATP [catalytic activity: ATP + H(2)O + cation(in) = ADP + orthophosphate + cation(out)]. Probable cation-transporter P-type ATPase B CtpB Rv0103c, (MTCY251.22c), len: 752 aa. Probable ctpB, cation-transporting P-type ATPase B (transmembrane protein), equivalent to CTPB_MYCLE|P46840 cation-transporting P-type ATPase B from Mycobacterium leprae (750 aa), FASTA scores: opt: 3615, E(): 0, (76.5% identity in 752 aa overlap). Also highly similar to others e.g. CAB96031.1|AL360055 putative metal transporter ATPase from Streptomyces coelicolor (753 aa); NP_241423.1|NC_002570 copper-transporting ATPase from Bacillus halodurans (806 aa); etc. Also highly similar to Z46257|MLACEA_7 aceA gene for isocitrate L from Mycobacterium leprae (750 aa), FASTA scores: opt: 3615, E():0, (76.5% identity in 752 aa overlap). And similar to MTCY251.11 from Mycobacterium tuberculosis, FASTA score: (68.3% identity in 742 aa overlap). Contains PS01047 Heavy-metal-associated domain, PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases), subfamily IB. P9WPT9 No cell wall and cell processes Q10877 P9WPT9 GO:0006754,GO:0004008,GO:0016021,GO:0046872,GO:0030001,GO:0005886,GO:0005524 3.6.3.- P9WPT9 ML2000,ML2000c MMAR_0269 Mb0106c NC_000962.3 Mycobrowser_v4 CDS 122317 123831 . + 0 Rv0104 Rv0104 Function unknown Conserved hypothetical protein Rv0104, (MTCY251.23), len: 504 aa. Conserved hypothetical protein, showing weak similarity with other cAMP-dependent protein kinases e.g. AAC37564.1|M65066 cAMP-dependent protein kinase RI-beta regulatory subunit from Homo sapiens (380 aa); etc. P9WM61 No conserved hypotheticals Q10898 P9WM61 GO:0003824,GO:0016021,GO:0008152,GO:0005886 P9WM61 MMAR_0285 Mb0107 NC_000962.3 Mycobrowser_v4 CDS 123980 124264 . - 0 Rv0105c rpmB1 Possibly involved in a translation mechanism. 50S ribosomal protein L28-1 RpmB1 Rv0105c, (MTCY251.24c), len: 94 aa. rpmB1, 50S ribosomal protein L28-1, highly similar to others e.g. Q9X8K8|R28B_STRCO 50S ribosomal protein L28-2 from Streptomyces coelicolor (78 aa); RL28_ECOLI|P02428 50s ribosomal protein l28 from Escherichia coli (77 aa), FASTA scores: opt: 167, E(): 6.2e-06, (40.7% identity in 59 aa overlap); etc. Also similar to MTCY63A_2 from Mycobacterium tuberculosis. Belongs to the L28P family of ribosomal proteins. P9WHB1 No information pathways P0A5V6 P9WHB1 GO:0003735,GO:0006412,GO:0005840 P9WHB1 MMAR_0292 Mb0108c NC_000962.3 Mycobrowser_v4 CDS 157847 158302 . + 0 Rv0130 htdZ Unknown Probable 3-hydroxyl-thioester dehydratase Rv0130, (MTCI5.04), len: 151 aa. Probable htdZ, 3-hydroxyl-thioester dehydratase. Forms single hot-dog fold, features R-specific hydratase motif, substrate unknown, forms homodimer. Shows structural similarity to six others in Mycobacterium tuberculosis (see Castell et al (2005) below). Similar to others e.g. AL096811|SCI30A_19 from Streptomyces coelicolor (153 aa), FASTA scores: opt: 639, E(): 0, (60.8% identity in 148 aa overlap). P9WNP3 No intermediary metabolism and respiration 2C2I P96807 P9WNP3 GO:0006631,GO:0016491,GO:0004300 4.2.1.17 P9WNP3 MMAR_0330 MSMEG_2201 Mb0135 NC_000962.3 Mycobrowser_v4 CDS 778990 779487 . + 0 Rv0678 Rv0678 Function unknown Conserved protein Rv0678, (MTV040.06), len: 165 aa. Conserved protein, showing weak similarity with AL049754|SCH10_10 hypothetical protein from Streptomyces coelicolor (152 aa), FASTA scores: opt: 149, E(): 0.0018, (22.9% identity in 140 aa overlap). I6Y8F7 No conserved hypotheticals I6Y8F7 I6Y8F7 MMAR_1007 Mb0697 NC_000962.3 Mycobrowser_v4 CDS 2258273 2258671 . + 0 Rv2010 vapC15 Unknown Toxin VapC15 Rv2010, (MTCY39.07c), len: 132 aa. VapC15, toxin, part of toxin-antitoxin (TA) operon with Rv2009, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). P9WF97 No virulence, detoxification, adaptation P64925 P9WF97 P9WF97 Mb2033 NC_000962.3 Mycobrowser_v4 CDS 125643 130541 . - 0 Rv0107c ctpI Cation-transporting ATPase; possibly catalyzes the transport of a cation (possibly magnesium) with the hydrolyse of ATP [catalytic activity: ATP + H(2)O + cation(in) = ADP + phosphate + cation(out)]. Probable cation-transporter ATPase I CtpI Rv0107c, (MTCY251.26c, MTV031.01c), len: 1632 aa. Probable ctpI, cation-transporting ATPase I P-type, highly similar to NP_302704.1|NC_002677 probable cation transport ATPase from Mycobacterium leprae (1609 aa); and similar to others e.g. CAB69720.1|AL137166 putative transport ATPase from Streptomyces coelicolor (1472 aa); ATA1_SYNY|P37367 cation-transporting ATPase pma1 from Synechocystis sp. (915 aa), FASTA scores: opt: 603, E(): 6.6e-29, (32.4% identity in 710 aa overlap); etc. Also similar to MTCY39.21c and MTCY22G10.22c from Mycobacterium tuberculosis, FASTA score: (34.4% identity in 796 aa overlap). Contains PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases). P9WPS5 No cell wall and cell processes Q10900 P9WPS5 GO:0006754,GO:0016021,GO:0015693,GO:0015444,GO:0046872,GO:0005886,GO:0005524 3.6.3.- P9WPS5 ML2671 Mb0110c,Mb0111c NC_000962.3 Mycobrowser_v4 CDS 130895 131104 . - 0 Rv0108c Rv0108c Unknown Hypothetical protein Rv0108c, (MTV031.02c), len: 69 aa. Hypothetical unknown protein. O53630 No conserved hypotheticals O53630 MMAR_0298 Mb0112c NC_000962.3 Mycobrowser_v4 CDS 133020 133769 . + 0 Rv0110 Rv0110 Unknown Probable conserved integral membrane protein Rv0110, (MTV031.04), len: 249 aa. Probable conserved integral membrane protein, similar to many e.g. AL079308|SCH69_25 from Streptomyces coelicolor (297 aa), FASTA scores: opt: 552, E(): 6.1e-29, (45.4% identity in 251 aa overlap); P54493|YQGP_BACSU hypothetical 56.4 KD protein from Bacillus subtilis (507 aa), FASTA scores: opt: 320, E(): 4e-15, (32.4% identity in 210 aa overlap); etc. O53632 No cell wall and cell processes O53632 GO:0016021 MSMEG_5036 Mb0114 NC_000962.3 Mycobrowser_v4 CDS 133950 136007 . + 0 Rv0111 Rv0111 Function unknown; probably involved in cellular metabolism. Possible transmembrane acyltransferase Rv0111, (MTV031.05), len: 685 aa. Possible transmembrane acyltransferase, equivalent to AA22904.1|AL035300 putative acyltransferase from Mycobacterium leprae (696 aa). Also similar to others e.g. C69975 acyltransferase homolog yrhL from Bacillus subtilis (634 aa), FASTA scores: opt: 520, E(): 4e-22, (36.4% identity in 382 aa overlap). Very similar to Mycobacterium tuberculosis proteins Rv0228, Rv1254, Rv1565c, etc. O53633 No intermediary metabolism and respiration O53633 O53633 GO:0016021,GO:0008415 2.3.1.- O53633 ML2670,ML2670c MMAR_0312 MSMEG_5537 Mb0115 NC_000962.3 Mycobrowser_v4 CDS 136289 137245 . + 0 Rv0112 gca Possibly involved in synthesis of a-band common antigen lipopolysaccharide. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose [catalytic activity: GDP-mannose = GDP-4-dehydro-6-deoxy-D-mannose + H2O]. Possible GDP-mannose 4,6-dehydratase Gca (GDP-D-mannose dehydratase) Rv0112, (MTV031.06), len: 318 aa. Possible gca, GDP-mannose 4,6-dehydratase, similar to others e g. U18320|PAU18320_1 GDP-D-mann from Pseudomonas aeruginosa (323 aa), FASTA scores: opt: 415, E(): 4.4e-21, (27.0% identity in 318 aa overlap). Similar to Rv3634c, Rv3784, etc from Mycobacterium tuberculosis. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Seems to belong to the GDP-mannose 4,6-dehydratase family. Cofactor: NAD(+). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53634 No cell wall and cell processes O53634 O53634 GO:0044237,GO:0050662,GO:0016491,GO:0008446 4.2.1.47 O53634 Mb0116 NC_000962.3 Mycobrowser_v4 CDS 677710 677925 . + 0 Rv0581 vapB26 Unknown Possible antitoxin VapB26 Rv0581, (MTV039.19), len: 71 aa. Possible vapB26, antitoxin, part of toxin-antitoxin (TA) operon with Rv0582, see Arcus et al. 2005. Showing weak similarity to other Mycobacterium tuberculosis proteins including P95003|Z83863|Rv2550c|MTCY159_6 conserved hypothetical protein (81 aa), FASTA scores: opt: 93, E(): 3.2, (25.7% identity in 70 aa overlap); Rv2871; Rv1241; etc. Also shows weak similarity to X05648|SGSPH_1 from Streptomyces glaucescens (77 aa), FASTA scores: opt: 92, E(): 3.6, (35.4% identity in 65 aa overlap). O53778 No virulence, detoxification, adaptation O53778 O53778 GO:0006355,GO:0003677 O53778 Mb0596 NC_000962.3 Mycobrowser_v4 CDS 138513 139673 . + 0 Rv0115 hddA Involved in biosynthesis of nucleotide-activated glycero-manno-heptose (D-alpha-D pathway) [catalytic activity: D-glycero-alpha,beta-D-manno-heptose 7-phosphate + ATP = D-glycero-alpha-D-manno-heptose 1,7-biphosphate]. Possible D-alpha-D-heptose-7-phosphate kinase HddA Rv0115, (MTV031.09), len: 386 aa. Possible hddA, D-alpha-D-heptose-7-phosphate kinase (see citation below), similar to several hypothetical proteins and sugar kinases e.g. AAK27850.1|AF324836_3 D-glycero-D-manno-heptose 7-phosphate kinase from Aneurinibacillus thermoaerophilus (341 aa); AAK80995.1|AE007802_11 Sugar kinase from Clostridium acetobutylicum (364 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53637 No cell wall and cell processes O53637 O53637 GO:0016301,GO:0016310,GO:0005524 2.-.-.- O53637 Mb0119 NC_000962.3 Mycobrowser_v4 CDS 140267 141022 . - 0 Rv0116c ldtA Involved in peptidoglycan synthesis. Catalyzes the formation of 3->3 crosslinks between peptidoglycan subunits. Probable L,D-transpeptidase LdtA Rv0116c, (MTV031.10c), len: 251 aa. Probable ldtA, L,D-transpeptidase, showing similarity to several hypothetical mycobacterial proteins e.g. Rv1433 from Mycobacterium tuberculosis (271 aa); and Q49706|B1496_F2_81|U00013 from Mycobacterium leprae (271 aa); to the C-terminal regions of others like Rv0192 from Mycobacterium tuberculosis (366 aa), FASTA scores: opt: 451, E(): 1.7e-21, (46.7% identity in 270 aa overlap); and Rv0192|Z97050|MTCI28_32 from Mycobacterium tuberculosis cosmid (366 aa), FASTA scores: opt: 699, E(): 0, (45.7% identity in 221 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). O53638 No cell wall and cell processes O53638 O53638 O53638 ML2664 MSMEG_3528 Mb0120c NC_000962.3 Mycobrowser_v4 CDS 141200 142144 . + 0 Rv0117 oxyS Could effect functions of OXYR during evolution. Oxidative stress response regulatory protein OxyS Rv0117, (MTV031.11), len: 314 aa. OxyS, oxidative stress response protein regulatory protein, LysR family (see citation below). Similar to many transcription regulators and OxyR, the oxidative stress response protein of many bacteria. Contains LysR family signature at N-terminus. Also contains helix-turn-helix motif at aa 16-37 (Score 1543, +4.44 SD). Belongs to the LysR family of transcriptional regulators. OXYR is required for the induction of a regulon of hydrogen peroxide inducible genes such as catalase, glutathione-reductase, etc. L7N677 No regulatory proteins O33175 L7N677 GO:0006350,GO:0003700,GO:0006355 L7N677 ML2663,ML2663c MMAR_0317 MSMEG_0156 Mb0121 NC_000962.3 Mycobrowser_v4 CDS 158315 159658 . - 0 Rv0131c fadE1 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE1 Rv0131c, (MTCI5.05c), len: 447 aa. Probable fadE1, acyl-CoA dehydrogenase, similar to many e.g. ACDS_HUMAN|P16219 acyl-CoA dehydrogenase short-chain specific precursor (412 aa), FASTA scores: opt: 522, E(): 1.4e-23, (30.1% identity in 425 aa overlap). Also highly similar to MTCI5_28 from Mycobacterium tuberculosis. P96808 No lipid metabolism P96808 P96808 GO:0003995,GO:0055114,GO:0050660 1.3.99.- P96808 MMAR_0331 Mb0136c NC_000962.3 Mycobrowser_v4 CDS 171215 172168 . + 0 Rv0145 Rv0145 Possible methyltransferase Possible S-adenosylmethionine-dependent methyltransferase Rv0145, (MTCI5.19), len: 317 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), highly similar to many e.g. CAC32172.1|AL583926 conserved hypothetical protein from Mycobacterium leprae (310 aa); and several Mycobacterium tuberculosis proteins e.g. Rv0726c, Rv0731c, etc. P9WFJ1 No lipid metabolism P96822 P9WFJ1 GO:0008168 2.1.1.- P9WFJ1 ML2641,ML2641c MMAR_0356 MSMEG_0093 Mb0150 NC_000962.3 Mycobrowser_v4 CDS 144049 145626 . + 0 Rv0119 fadD7 Function unknown, but involved in lipid degradation. Probable fatty-acid-CoA ligase FadD7 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv0119, (MTV031.13-MTCI418B.01), len: 525 aa. Probable fadD7, fatty-acid-CoA synthetase, similar to 4-coumarate:CoA ligase of many organisms e.g. U39405|PTU39405_1 4-coumarate:CoA ligase from Pinus taedaxylem (537 aa), FASTA scores: opt: 483, E(): 8.3e-22, (28.2% identity in 440 aa overlap). Contains PS00455 Putative AMP-binding domain signature. O07169 No lipid metabolism O07169 O07169 GO:0008152,GO:0016874 6.2.1.- O07169 ML2661,ML2661c MSMEG_5295 Mb0123 NC_000962.3 Mycobrowser_v4 CDS 145627 147771 . - 0 Rv0120c fusA2 Involved in translation mechanism. This protein may promote the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. Probable elongation factor G FusA2 (EF-G) Rv0120c, (MTCI418B.02c), len: 714 aa. Probable fusA2 (alternate gene name: fus2), elongation factor G, highly similar to others e.g. EFG_ECOLI|P02996 elongation factor G (ef-g) from Escherichia coli (703 aa), FASTA scores: opt: 1049, E(): 0, (32.5% identity in 717 aa overlap). Also similar to fusA1|MTCY210.01 from Mycobacterium tuberculosis FASTA score: (39.1% identity in 299 aa overlap); and P30767|EFG_MYCLE elongation factor G (EF-G) from Mycobacterium leprae (701 aa), FASTA score: (31.7% identity in 710 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the GTP-binding elongation factor family, EF-G/EF-2 subfamily. P9WNM9 No information pathways O07170 P9WNM9 GO:0003924,GO:0005525 P9WNM9 ML2660 MMAR_0321 MSMEG_6535 Mb0124c,Mb0125c NC_000962.3 Mycobrowser_v4 CDS 147908 148342 . - 0 Rv0121c Rv0121c Function unknown Conserved protein Rv0121c, (MTCI418B.03c), len: 144 aa. Conserved protein, showing some similarity with others proteins from Mycobacterium tuberculosis e.g. Rv1155, Rv1875, Rv2074, etc. O07171 No conserved hypotheticals O07171 O07171 GO:0010181 O07171 MMAR_0322 MSMEG_6526 Mb0126c NC_000962.3 Mycobrowser_v4 CDS 148491 148859 . + 0 Rv0122 Rv0122 Unknown Hypothetical protein Rv0122, (MTCI418B.04), len: 122 aa. Hypothetical unknown protein. O07172 No conserved hypotheticals O07172 O07172 Mb0127 NC_000962.3 Mycobrowser_v4 CDS 148856 149224 . + 0 Rv0123 Rv0123 Unknown Unknown protein Rv0123, (MTCI418B.05), len: 122 aa. Unknown protein. O07173 No conserved hypotheticals O07173 O07173 GO:0006355,GO:0003677 Mb0128 NC_000962.3 Mycobrowser_v4 CDS 149533 150996 . + 0 Rv0124 PE_PGRS2 Function unknown PE-PGRS family protein PE_PGRS2 Rv0124, (MTCI418B.06), len: 487 aa. PE_PGRS2, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), highly similar to many e.g. Y0DP_MYCTU|Q50615 from Mycobacterium tuberculosis (498 aa), FASTA scores: opt: 1730, E(): 0, (60.7% identity in 504 aa overlap). Q79G08 No PE/PPE Q79G08 Q79G08 Q79G08 MMAR_0572,MMAR_2053,MMAR_2097,MMAR_2248,MMAR_2492,MMAR_2596,MMAR_2709,MMAR_2970,MMAR_3199,MMAR_4127,MMAR_4149,MMAR_4548,MMAR_4561,MMAR_4562 MSMEG_3202 Mb0129 NC_000962.3 Mycobrowser_v4 CDS 289345 289782 . + 0 Rv0240 vapC24 Unknown Possible toxin VapC24. Contains PIN domain. Rv0240, (MTV034.06), len: 145 aa. Possible vapC24, toxin, part of toxin-antitoxin (TA) operon with Rv0239, contains PIN domain, weak similarity with Rv3697c from Mycobacterium tuberculosis (145 aa). See Arcus et al. 2005. FASTA scores: opt: 145, E(): 7.6e-05, (28.0% identity in 143 aa overlap). P9WF87 No virulence, detoxification, adaptation O53663 P9WF87 P9WF87 Mb0246 NC_000962.3 Mycobrowser_v4 CDS 677922 678329 . + 0 Rv0582 vapC26 Unknown Possible toxin VapC26. Contains PIN domain. Rv0582, (MTV039.20), len: 135 aa. Possible vapC26, toxin, part of toxin-antitoxin (TA) operon with Rv0581, contains PIN domain, see Arcus et al. 2005. O53779 No virulence, detoxification, adaptation O53779 O53779 O53779 Mb0597 NC_000962.3 Mycobrowser_v4 CDS 154232 155599 . + 0 Rv0127 mak Catalyzes the transfer of a phosphate group from ATP to maltose to produce maltose-1-phosphate [catalytic activity: ATP + maltose = ADP + maltose-1-phosphate] Maltokinase Mak Rv0127, (MTCI418B.09, MTCI5.01), len: 455 aa. Mak, maltokinase; highly similar to various proteins e.g. AJ0012|SCJ001205_4 hypothetical protein from Streptomyces coelicolor A3(2) (464 aa), FASTA scores: opt: 412, E(): 1.1e-19, (40.6% identity in 485 aa overlap); AJ0012|SCJ001206_5 hypothetical protein from Streptomyces coelicolor A3(2) (453 aa), FASTA scores: opt: 403, E(): 4.3 e-19, (36.5% identity in 455 aa overlap). O07177 No virulence, detoxification, adaptation O07177 O07177 O07177 ML2657,ML2657c MMAR_0326 MSMEG_6514 Mb0132 NC_000962.3 Mycobrowser_v4 CDS 155667 156446 . + 0 Rv0128 Rv0128 Unknown Probable conserved transmembrane protein Rv0128, (MTCI5.02), len: 259 aa. Probable conserved transmembrane protein, with some similarity to Rv3064c and other bacterial proteins e.g. AAK85977.1|AE007957|AGR_C_254p from Agrobacterium tumefaciens (206 aa). P96805 No cell wall and cell processes P96805 P96805 GO:0016021 ML2656,ML2656c MMAR_0327 Mb0133 NC_000962.3 Mycobrowser_v4 CDS 208417 208971 . + 0 Rv0177 Rv0177 Unknown Probable conserved Mce associated protein Rv0177, (MTCI28.17), len: 184 aa. Probable conserved Mce-associated protein, equivalent to CAC32129.1|AL583926 conserved membrane protein from Mycobacterium leprae (184 aa). Also similar to mce-associated proteins from Mycobacterium tuberculosis e.g. Rv1363c, Rv1973, Rv3493c, etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O07421 No conserved hypotheticals O07421 ML2597 MMAR_0420 MSMEG_0142 Mb0183 NC_000962.3 Mycobrowser_v4 CDS 257783 258856 . - 0 Rv0215c fadE3 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE3 Rv0215c, (MTCY08D5.10c), len: 357 aa. Probable fadE3, acyl- dehydrogenase, similar to many e.g. ACDB_BACSU|P45857 acyl-CoA dehydrogenase from B. subtilis (379 aa), FASTA scores: opt: 812, E(): 0, (39.5% identity in 354 aa overlap). P96397 No lipid metabolism P96397 P96397 GO:0003995,GO:0055114,GO:0050660 1.3.99.- P96397 ML2626,ML2626c MMAR_0455,MMAR_4161 MSMEG_1991 Mb0221c NC_000962.3 Mycobrowser_v4 CDS 475816 476184 . + 0 Rv0397 Rv0397 Function unknown Conserved 13E12 repeat family protein Rv0397, (MTCY04D9.09), len: 122 aa. Part of 13E12 repeat family of conserved Mycobacterium tuberculosis proteins, similar to downstream Rv0393|Z84725|MTCY4D9_5 conserved 13E12 repeat family protein (441 aa), FASTA scores: E(): 0, (87.7% identity in 122 aa overlap). P95205 No insertion seqs and phages P95205 P95205 Mb0403 NC_000962.3 Mycobrowser_v4 CDS 2263426 2264001 . + 0 Rv2016 Rv2016 Unknown Hypothetical protein Rv2016, (MTV018.03), len: 191 aa. Hypothetical protein. O53462 No conserved hypotheticals O53462 Mb2039 NC_000962.3 Mycobrowser_v4 CDS 159700 160782 . - 0 Rv0132c fgd2 Catalyzes oxidation of glucose-6-phosphate to 6-phosphogluconolactone using coenzyme F420 (an *-hydroxy-5-deazaflavin derivative) as the electron acceptor. Putative F420-dependent glucose-6-phosphate dehydrogenase Fgd2 Rv0132c, (MTCI5.06c), len: 360 aa. Putative fgd2, F420-dependent glucose-6-phosphate dehydrogenase, highly similar to many from Mycobacteria e.g. AAD38167|g5031431 from Mycobacterium chelonae. Also similar to MJ1534|Q58929 N5,N10-methylene tetrahydromethanopterin reductase from methanococcus jannaschii (342 aa), FASTA scores: opt: 285, E(): 7.9e-11, (28.4% identity in 292 aa overlap). And also similar to Rv0953c, Rv0791c, etc from Mycobacterium tuberculosis. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. P96809 No intermediary metabolism and respiration P96809 P96809 GO:0016491,GO:0055114 1.-.-.- P96809 Mb0137c NC_000962.3 Mycobrowser_v4 CDS 160869 161474 . + 0 Rv0133 Rv0133 Acetylation, substrate unknown GCN5-related N-acetyltransferase Rv0133, (MTCI5.07), len: 201 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain in C-terminal part. See Vetting et al. 2005. Highly similar to others e.g. PUAC_STRLP|P13249 puromycyn N-acetyltransferase (199 aa), FASTA scores: opt: 341, E(): 1.8e-16, (33.3% identity in 201 aa overlap). P96810 No intermediary metabolism and respiration P96810 P96810 GO:0008152,GO:0008080 2.3.1.- P96810 MMAR_0332 MSMEG_4563 Mb0138 NC_000962.3 Mycobrowser_v4 CDS 161771 162673 . + 0 Rv0134 ephF Thought to be involved in detoxification reactions following oxidative damage to lipids [catalytic activity: an epoxide + H(2)O = a glycol]. Possible epoxide hydrolase EphF (epoxide hydratase) (arene-oxide hydratase) Rv0134, (MTCI5.08), len: 300 aa. Possible ephE, epoxide hydrolase (see citation below), similar to others e.g. Q39856 epoxide hydrolase (341 aa), FASTA scores: opt: 369, E(): 4.6e-17, (27.2% identity in 335 aa overlap); etc. Also similar to MTCY09F9.26c from Mycobacterium tuberculosis (29.5% identity in 346 aa overlap). P96811 No virulence, detoxification, adaptation P96811 P96811 GO:0016787 3.3.2.3 P96811 MMAR_0342 Mb0139 NC_000962.3 Mycobrowser_v4 CDS 196861 197658 . + 0 Rv0167 yrbE1A Unknown Conserved integral membrane protein YrbE1A Rv0167, (MTCI28.07), len: 265 aa. YrbE1A, unknown integral membrane protein, part of mce1 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07791|Rv0587|MTCY19H5.35|yrbE2A (265 aa); O53965|Rv1964|MTV051.02|yrbE3A (265 aa); etc. Also highly similar or similar to conserved hypothetical integral membrane proteins of yrbEA type, e.g. NP_302654.1|NC_002677 conserved membrane protein from Mycobacterium leprae (267 aa); P45030|YRBE_HAEIN|HI1086 hypothetical protein from Haemophilus influenzae (261 aa), FASTA scores: opt: 328, E(): 1.8e-15, (26.6% identity in 244 aa overlap); etc. O07412 No virulence, detoxification, adaptation O07412 O07412 O07412 ML2587 MMAR_0410 MSMEG_0132 Mb0173 NC_000962.3 Mycobrowser_v4 CDS 237892 238395 . - 0 Rv0201c Rv0201c Function unknown Conserved protein Rv0201c, (MTV033.09c), len: 167 aa. Conserved protein, equivalent to Z95398|MLCL622.15c from Mycobacterium leprae (170 aa), FASTA scores: opt: 646, E(): 0, (63.9% identity in 158 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53652 No conserved hypotheticals O53652 O53652 ML2616,ML2616c MMAR_0441 MSMEG_0240 Mb0207c NC_000962.3 Mycobrowser_v4 CDS 2833510 2833761 . - 0 Rv2517c Rv2517c Unknown Unknown protein Rv2517c, (MTV009.02c), len: 83 aa. Unknown protein. Equivalent to AAK46899 from Mycobacterium tuberculosis strain CDC1551 (97 aa) but shorter 14 aa. O53222 No conserved hypotheticals O53222 Mb2546c NC_000962.3 Mycobrowser_v4 ncRNA 293604 293705 . + 0 MTB000051 F6 Unknown Putative small regulatory RNA F6, putative small regulatory RNA (See Arnvig and Young, 2009; DiChiara et al., 2010). Alternate 3'-ends at positions 293641 and 293661. No stable RNAs NC_000962.3 Mycobrowser_v4 CDS 165827 166849 . + 0 Rv0139 Rv0139 Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0139, (MTCI5.13), len: 340 aa. Possible oxidoreductase, similar to others e.g. O34285|HPNA HPNA protein from Zymomonas mobilis (337 aa), FASTA scores: opt: 507, E (): 5.8e-27, (31.1% identity in 328 aa overlap); TRE_STRGR|P29782 dtdp-glucose 4,6-dehydratase (328 aa), FASTA scores: opt: 254, E(): 2.6e-10, (29.0% identity in 307 aa overlap). P96816 No intermediary metabolism and respiration P96816 P96816 GO:0050662,GO:0055114,GO:0016491,GO:0044237 1.-.-.- P96816 ML2645,ML2645c MMAR_0349 MSMEG_6474 Mb0144 NC_000962.3 Mycobrowser_v4 CDS 170284 171126 . + 0 Rv0144 Rv0144 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (possibly TetR-family) Rv0144, (MTCI5.18), len: 280 aa. Probable transcriptional regulator, possibly TetR family. Has region similar to others e.g. Q59431|UIDR_ECOLI|GUSR|B1618|Z2623|ECS2326 UID operon repressor (GUS operon) from Escherichia coli strains K12 and O157:H7 (196 aa), FASTA scores: opt: 214, E(): 1.1e-06, (26.0% identity in 196 aa overlap). Contains probable helix-turn helix motif from aa 109-130 (Score 1463, +4.17 SD). Could belong to the TetR/AcrR family of transcriptional regulators. P96821 No regulatory proteins P96821 P96821 GO:0006350,GO:0003700,GO:0006355 P96821 ML2642,ML2642c MMAR_0355 MSMEG_0092 Mb0149 NC_000962.3 Mycobrowser_v4 CDS 172211 173143 . + 0 Rv0146 Rv0146 Possible methyltransferase Possible S-adenosylmethionine-dependent methyltransferase Rv0146, (MTCI5.20), len: 310 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), highly similar to others e.g. AC30975.1|AL583924 conserved hypothetical protein from Mycobacterium leprae (304 aa); and several Mycobacterium tuberculosis proteins e.g. Rv0726c, Rv0731c, etc. P9WFJ3 No lipid metabolism P96823 P9WFJ3 GO:0008168 2.1.1.- P9WFJ3 ML2640,ML2640c Mb0151 NC_000962.3 Mycobrowser_v4 CDS 223607 223909 . + 0 Rv0192A Rv0192A Function unknown Conserved secreted protein Rv0192A, len: 100 aa. Probable N-terminal part of Rv0192, which is member of family P5.17 with Rv0116c, Rv1433, Rv2518c, Rv0483. These are all predicted to be exported/membrane proteins. Rv0192A has typical N-terminal signal peptide which is functional and was identified by PhoA fusion screens: O52054 PGB14T-O1 precursor (fragment 45 AA) (see Chubb et al., 1998). Since Rv0192 misses a signal peptide this suggests that there is a frameshift in the region of the overlap with Rv0192 but none found on reinspection of sequence. Q79FZ8 No cell wall and cell processes Q79FZ8 Mb0198 NC_000962.3 Mycobrowser_v4 CDS 1027104 1027685 . + 0 Rv0921 Rv0921 Prevents the cointegration of foreign DNA before integration into the chromosome. Possible resolvase Rv0921, (MTCY21C12.15), len: 193 aa. Possible resolvase for IS1535, highly similar to many bacterial resolvases e.g. MTCY274.17c|YX1C_MYCTU Q10831 from Mycobacterium tuberculosis (295 aa), FASTA scores: opt: 537, E(): 5.7e-29, (51.8% identity in 166 aa overlap). Presents an helix turn helix motif. I6WZS4 No insertion seqs and phages O05913 I6WZS4 GO:0006310,GO:0000150,GO:0003677 I6WZS4 Mb0945 NC_000962.3 Mycobrowser_v4 promoter 174782 174803 . + 0 Rv0148 Rv0148 Function unknown; possibly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv0148, (MTCI5.22), len: 286 aa. Probable short-chain dehydrogenase, similar to others, in particular Estradiol 17 beta-dehydrogenases, e.g. DHB4_MOUSE|P51660 estradiol 17 beta-dehydrogenase 4 (735 aa), FASTA scores: opt: 952, E(): 0, (52.5% identity in 276 aa overlap). Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. P96825 No intermediary metabolism and respiration P96825 P96825 GO:0055114,GO:0016491 1.-.-.- P96825 Mb0153 NC_000962.3 Mycobrowser_v4 CDS 174833 175693 . + 0 Rv0148 Rv0148 Function unknown; possibly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv0148, (MTCI5.22), len: 286 aa. Probable short-chain dehydrogenase, similar to others, in particular Estradiol 17 beta-dehydrogenases, e.g. DHB4_MOUSE|P51660 estradiol 17 beta-dehydrogenase 4 (735 aa), FASTA scores: opt: 952, E(): 0, (52.5% identity in 276 aa overlap). Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. P96825 No intermediary metabolism and respiration P96825 P96825 GO:0055114,GO:0016491 1.-.-.- P96825 Mb0153 NC_000962.3 Mycobrowser_v4 CDS 175700 176668 . + 0 Rv0149 Rv0149 Possibly binds NADP and acts through a one-electron transfer process. Quinones are supposed to be the best substrates. May act in the detoxification of xenobiotics [catalytic activity: NADPH + quinone = NADP+ + semiquinone] Possible quinone oxidoreductase (NADPH:quinone oxidoreductase) (zeta-crystallin) Rv0149, (MTCI5.23), len: 322 aa. Possible quinone oxidoreductase, similar to others oxidoreductases e.g. Q08257 quinone oxidoreductase (329 aa), FASTA scores: opt: 397, E(): 3.2e-18, (28.4% identity in 328 aa overlap); SCHCOADH_4 from Streptomyces coelicolor. Also similar to many proteins from Mycobacterium tuberculosis. Contains PS01162 Quinone oxidoreductase / zeta-crystallin signature. Belongs to the zinc-containing alcohol dehydrogenase family, quinone oxidoreductase subfamily. P96826 No intermediary metabolism and respiration P96826 P96826 GO:0055114,GO:0008270,GO:0003960 1.6.5.5 P96826 ML2638,ML2638c MMAR_0361 MSMEG_0097 Mb0154 NC_000962.3 Mycobrowser_v4 CDS 176665 176952 . - 0 Rv0150c Rv0150c Function unknown Conserved hypothetical protein Rv0150c, (MTCI5.24c), len: 95 aa. Conserved hypothetical protein, showing some similarity with C-terminus of O53949|Rv1800|MTV049.22 PPE-family protein from Mycobacterium tuberculosis (655 aa), FASTA score: (36.5% identity in 104 aa overlap). P96827 No conserved hypotheticals P96827 P96827 Mb0155c NC_000962.3 Mycobrowser_v4 CDS 209703 210812 . - 0 Rv0179c lprO Unknown Possible lipoprotein LprO Rv0179c, (MTCI28.19c), len: 369 aa. Possible lprO, lipoprotein (visibly not conserved). Contains possible N-terminal signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O07423 No cell wall and cell processes O07423 ML2599,ML2599c MMAR_0422 MSMEG_0210 Mb0185c NC_000962.3 Mycobrowser_v4 CDS 214969 215718 . + 0 Rv0184 Rv0184 Function unknown Conserved hypothetical protein Rv0184, (MTCI28.24), len: 249 aa. Conserved hypothetical protein, equivalent to CAC32136.1|AL583926 conserved hypothetical protein from Mycobacterium leprae (249 aa); and C-terminus highly similar to CAB08793.1|Z95398 conserved hypothetical protein from Mycobacterium leprae (145 aa), FASTA scores: E(): 0, (75.2 identity in 145 aa overlap). Also similar to 049841|SCE9_39|T36358 hypothetical protein from Streptomyces coelicolor (418 aa), FASTA scores: opt: 231, E(): 8.1e-08, (30.4% identity in 270 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O07428 No conserved hypotheticals O07428 ML2604 MMAR_0428 MSMEG_0222 Mb0190 NC_000962.3 Mycobrowser_v4 CDS 424269 424694 . - 0 Rv0354c PPE7 Function unknown PPE family protein PPE7 Rv0354c, (MTCY13E10.14c), len: 141 aa. PPE7, Member of the Mycobacterium tuberculosis PPE family, similar to others e.g. MTCY63_9 from Mycobacterium tuberculosis (2411 aa), FASTA scores: E(): 3.6e-11, (47.6% identity in 103 aa overlap). Possible continuation of ORF upstream, but no sequence error apparent. L0T545 No PE/PPE L0T545 Mb0362c NC_000962.3 Mycobrowser_v4 CDS 181987 183198 . - 0 Rv0154c fadE2 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE2 Rv0154c, (MTCI5.28c), len: 403 aa. Probable fadE2, acyl-CoA dehydrogenase, similar to many e.g. C-terminal region of O01590 acyl-CoA dehydrogenase (974 aa), FASTA scores: opt: 1150, E(): 0, (50.0% identity in 402 aa overlap); ACDS_MEGEL|Q06319 acyl-CoA dehydrogenase (short-chain) (383 aa), FASTA score: (35.0% identity in 306 aa overlap). Could belong to the acyl-CoA dehydrogenases family. P96831 No lipid metabolism P96831 P96831 GO:0003995,GO:0055114,GO:0050660 1.3.99.- P96831 ML2637 Mb0159c NC_000962.3 Mycobrowser_v4 CDS 183622 184722 . + 0 Rv0155 pntAa The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [catalytic activity: NADPH + NAD+ = NADP+ + NADH]. Probable NAD(P) transhydrogenase (subunit alpha) PntAa [first part; catalytic part] (pyridine nucleotide transhydrogenase subunit alpha) (nicotinamide nucleotide transhydrogenase subunit alpha) Rv0155, (MTCI5.29), len: 366 aa. Probable pntAa, first part of NAD(P) transhydrogenase subunit alpha, similar to N-terminus of others e.g. PNTA_ECOLI|P07001|P76888|B1603 NAD (P) transhydrogenase subunit alpha from Escherichia coli strain K12 (510 aa), FASTA scores: opt: 921, E(): 0, (42.1% identity in 361 aa overlap); proton-translocating nicotinamide nucleotide transhydrogenase subunit PNTAA. P96832 No intermediary metabolism and respiration P96832 P96832 GO:0055114,GO:0008750 1.6.1.2 P96832 ML2636,ML2636c MMAR_0377 MSMEG_0152 Mb0160 NC_000962.3 Mycobrowser_v4 CDS 210892 212250 . - 0 Rv0180c Rv0180c Unknown Probable conserved transmembrane protein Rv0180c, (MTCI28.20c), len: 452 aa. Probable conserved transmembrane protein, equivalent to CAC32132.1|AL583926 probable conserved membrane protein from Mycobacterium leprae (465 aa). Shows some similarity with others membrane proteins e.g. AL096849|SCI11_29 from Streptomyces coelicolor (354 aa), FASTA scores: opt: 190, E(): 0.00067, (25.9% identity in 409 aa overlap). O07424 No cell wall and cell processes O07424 GO:0016021 O07424 ML2600,ML2600c MMAR_0423 Mb0186c NC_000962.3 Mycobrowser_v4 CDS 224724 226571 . - 0 Rv0193c Rv0193c Unknown Hypothetical protein Rv0193c, (MTV033.01c-MTCI28.32), len: 615 aa. Hypothetical unknown protein. O07437 No conserved hypotheticals O07437 O07437 MMAR_5224 Mb0199c NC_000962.3 Mycobrowser_v4 CDS 249038 250123 . + 0 Rv0209 Rv0209 Unknown Hypothetical protein Rv0209, (MTCY08D5.04), len: 361 aa. Hypothetical unknown protein. P96391 No conserved hypotheticals P96391 P96391 MMAR_0449 MSMEG_0253 Mb0215 NC_000962.3 Mycobrowser_v4 CDS 1593505 1593978 . + 0 Rv1419 Rv1419 Unknown Unknown protein Rv1419, (MTCY21B4.37), len: 157 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). P9WLX9 No conserved hypotheticals P64849 P9WLX9 GO:0005529,GO:0016021 P9WLX9 MMAR_3986 Mb1454 NC_000962.3 Mycobrowser_v4 CDS 184723 185055 . + 0 Rv0156 pntAb The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [catalytic activity: NADPH + NAD+ = NADP+ + NADH]. Probable NAD(P) transhydrogenase (subunit alpha) PntAb [second part; integral membrane protein] (pyridine nucleotide transhydrogenase subunit alpha) (nicotinamide nucleotide transhydrogenase subunit alpha) Rv0156, (MTCI5.30), len: 110 aa. Probable pntAb, second part of NAD(P) transhydrogenase subunit alpha, integral membrane protein, similar to C-terminus of others e.g. Q59764 nicotinamide nucleotide transhydrogenase subunit PNTAB (139 aa), FASTA scores: opt: 247, E(): 1.9e-11, (45.5% identity in 88 aa overlap). P96833 No intermediary metabolism and respiration P96833 P96833 ML2635,ML2635c MMAR_0378 MSMEG_0151 Mb0161 NC_000962.3 Mycobrowser_v4 CDS 186785 187429 . + 0 Rv0158 Rv0158 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (possibly TetR-family) Rv0158, (MTV032.01), len: 214 aa. Probable transcriptional regulator, possibly TetR family, showing weak similarity to various transcriptional activators and repressors e.g. P32398|YIXD_BACSU|YHGD hypothetical transcriptional regulatory protein from Bacillus subtilis (191 aa), FASTA scores: opt:172, E(): 2.4e-05, (23.0% identity in 191 aa overlap). Contains helix-turn-helix motif at aa 32-53 (Score 1296, +3.60 SD). Could belong to the TetR/AcrR family of transcriptional regulators. O53641 No regulatory proteins O53641 O53641 GO:0006355,GO:0006350,GO:0003700,GO:0016481 O53641 ML2633,ML2633c MMAR_0381 MSMEG_0120 Mb0163 NC_000962.3 Mycobrowser_v4 CDS 187433 188839 . - 0 Rv0159c PE3 Function unknown PE family protein PE3 Rv0159c, (MTV032.02c), len: 468 aa. PE3, Member of the Mycobacterium tuberculosis PE family (see citation below), similar to many other PE proteins e.g. O06828 from Mycobacterium tuberculosis (528 aa), FASTA scores: opt: 1163, E(): 0, (45.8% identity in 467 aa overlap). Also highly similar to upstream MTV032_3, and to MTCI5_25, MTCI5_26, MTV049_ 21, MTCY1A10_26, etc. Q79G04 No PE/PPE Q79G04 Q79G04 Q79G04 MMAR_0383 Mb0164c NC_000962.3 Mycobrowser_v4 CDS 188931 190439 . - 0 Rv0160c PE4 Function unknown PE family protein PE4 Rv0160c, (MTV032.03c), len: 502 aa. PE4, Member of the Mycobacterium tuberculosis PE family (see citation below), similar to many other PE proteins e.g. Z92770|MTCI5_26c from Mycobacterium tuberculosis (525 aa), FASTA scores: opt: 816, E(): 0, (41.4% identity in 367 aa overlap); C-terminal region of O06801|RV1768|MTCY28.34 from Mycobacterium tuberculosis (618 aa), FASTA scores: opt: 417, E(): 6.7e-18, (53.5% identity in 142 aa overlap). Also highly similar to downstream ORF MTV032_2. L7N661 No PE/PPE Q7DAC9 L7N661 L7N661 ML2632 MMAR_0384 Mb0165c NC_000962.3 Mycobrowser_v4 CDS 212277 213011 . - 0 Rv0181c Rv0181c Function unknown Conserved hypothetical protein Rv0181c, (MTCI28.21c), len: 244 aa. Conserved hypothetical protein, highly similar to other hypothetical proteins e.g. YHHW_ECOLI|P46852 hypothetical 26.3 kd protein from Escherichia coli (231 aa), FASTA scores: opt: 479, E(): 1.2e-29, (37.3% identity in 233 aa overlap); P73623|SLL1773 hypothetical 25.7 kDa protein from Synechocystis sp. strain PCC 6803 (232 aa), FASTA score: (39.1% identity in 233 aa overlap). P9WI85 No conserved hypotheticals P65724 P9WI85 P9WI85 ML2601,ML2601c MMAR_0424 MSMEG_0215 Mb0187c NC_000962.3 Mycobrowser_v4 CDS 262254 262802 . + 0 Rv0219 Rv0219 Unknown Probable conserved transmembrane protein Rv0219, (MTCY08D5.14), len: 182 aa. Probable conserved transmembrane protein, showing similarity with CAB76992.1|AL159178 putative lipoprotein from Streptomyces coelicolor (163 aa). P96401 No cell wall and cell processes P96401 GO:0016021 MMAR_0462 Mb0225 NC_000962.3 Mycobrowser_v4 CDS 552026 552550 . + 0 Rv0461 Rv0461 Unknown Probable transmembrane protein Rv0461, (MTV038.05), len: 174 aa (start uncertain). Probable transmembrane protein. Nucleotide position 552085 in the genome sequence has been corrected, A:G resulting in Q20Q. I6X961 No cell wall and cell processes I6X961 GO:0016021 ML2386 Mb0470 NC_000962.3 Mycobrowser_v4 CDS 191984 193135 . - 0 Rv0162c adhE1 Dehydrogeneses a alcohol (OXIDO-reduction) [catalytic activity: an alcohol + NAD+ = an aldehyde or ketone + NADH]. Probable zinc-type alcohol dehydrogenase (E subunit) AdhE1 Rv0162c, (MTCI28.02c), len: 383 aa. Probable adhE1, zinc-type alcohol dehydrogenase, similar to others e.g. ADH_MACMU|P28469 alcohol dehydrogenase alpha chain (374 aa), FASTA scores: opt: 619, E(): 0, (34.7% identity in 363 aa overlap). Also similar to other alcohol dehydrogenases from Mycobacterium tuberculosis e.g. MTCY369.06c FASTA score: (34.0% identity in 365 aa overlap), MTV022_9 FASTA score: (35.0% identity in 371 aa overlap). Contains PS00059 Zinc-containing alcohol dehydrogenases signature. Belongs to the zinc-containing alcohol dehydrogenase family, class-I subfamily. Cofactor: zinc. L7N6B3 No intermediary metabolism and respiration Q7DAC8 L7N6B3 GO:0055114,GO:0008270,GO:0004022 1.1.1.1 L7N6B3 MMAR_0405 MSMEG_0127 Mb0167c NC_000962.3 Mycobrowser_v4 CDS 193117 193572 . + 0 Rv0163 Rv0163 Function unknown Conserved protein Rv0163, (MTCI28.03), len: 151 aa. Conserved protein, similar to others e.g. Q44017 hypothetical 16.6 KDA protein in GBD 5'region (ORF6)from Alcaligenes eutrophus (145 aa), FASTA scores: opt: 155, E(): 0.0002, (26.6% identity in 139 aa overlap). Also weak similarity with MTV008.31c|Rv2475c|B70867 from Mycobacterium tuberculosis (138 aa). O07408 No conserved hypotheticals O07408 O07408 O07408 MMAR_0406 MSMEG_0128 Mb0168 NC_000962.3 Mycobrowser_v4 CDS 193626 194111 . + 0 Rv0164 TB18.5 Function unknown Conserved protein TB18.5 Rv0164, (MTCI28.04), len: 161 aa. TB18.5, conserved protein, equivalent to CAB08818.1|Z95398 hypothetical protein from Mycobacterium leprae (156 aa) FASTA scores: opt: 762, E(): 0, (76.3% identity in 152 aa overlap). Some similarity to Rv2185c, Rv0854, Rv0857 from Mycobacterium tuberculosis. Alternative start codon has been suggested. 3' part corrected since first submission (-24 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). L7N657 No conserved hypotheticals Q8VKQ8 L7N657 L7N657 ML2629,ML2629c MMAR_0407 MSMEG_0129 Mb0169 NC_000962.3 Mycobrowser_v4 CDS 194144 194815 . - 0 Rv0165c mce1R Involved in transcriptional mechanism Probable transcriptional regulatory protein Mce1R (probably GntR-family) Rv0165c, (MTCI28.05c), len: 223 aa. Probable mce1R, transcriptional regulator, GntR family (See Casali et al., 2006), showing some similarity to several e.g. NTRA_CHELE|P54988 nta operon transcriptional regulator (231 aa), FASTA scores: opt: 154, E(): 0.00058, (32.0% identity in 125 aa overlap); P46833|GNTR_BACLI gluconate operon transcriptional repressor from Bacillus licheniformis (243 aa); GNTR_BACSU gluconate operon repressor from Bacillus subtilis (243 aa). Also similar to Rv0043c from Mycobacterium tuberculosis. Seems to belong to the GntR family of transcriptional regulators. Start changed since first submission (-41 aa). Q79G00 No regulatory proteins Q79G00 Q79G00 GO:0006355,GO:0006350,GO:0003700,GO:0005622 Q79G00 MMAR_0408 MSMEG_1995 Mb0170c,Mb0171c NC_000962.3 Mycobrowser_v4 CDS 197660 198529 . + 0 Rv0168 yrbE1B Unknown Conserved integral membrane protein YrbE1B Rv0168, (MTCI28.08), len: 289 aa. YrbE1B, unknown integral membrane protein, part of mce1 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07790|Rv0588|MTCY19H5.34|yrbE2B (295 aa); O53966|Rv1965|MTV051.03|yrbE3B (271 aa); etc. Also highly similar to conserved hypothetical integral membrane proteins of the yrbEB type, e.g. NP_302655.1|NC_002677 conserved membrane protein from Mycobacterium leprae (289 aa); P45030|YRBE_HAEIN|HI1086 hypothetical protein from Haemophilus influenzae (261 aa), FASTA scores: opt: 223, E(): 7.6e-07, (23.7% identity in 257 aa overlap); etc. L0T2Q9 No virulence, detoxification, adaptation O07413 L0T2Q9 L0T2Q9 ML2588 MMAR_0411 MSMEG_0133 Mb0174 NC_000962.3 Mycobrowser_v4 CDS 475350 475742 . + 0 Rv0396 Rv0396 Unknown Hypothetical protein Rv0396, (MTCY04D9.08), len: 130 aa. Hypothetical unknown protein. P95204 No conserved hypotheticals P95204 Mb0402 NC_000962.3 Mycobrowser_v4 CDS 704752 705909 . - 0 Rv0610c Rv0610c Unknown Hypothetical protein Rv0610c, (MTCY19H5.11), len: 385 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6Y481 No conserved hypotheticals I6Y481 I6Y481 Mb0627c NC_000962.3 Mycobrowser_v4 CDS 1836387 1836830 . - 0 Rv1632c Rv1632c Unknown Hypothetical protein Rv1632c, (MTCY01B2.24c), len: 147 aa. Hypothetical unknown protein. O06149 No conserved hypotheticals O06149 O06149 O06149 MMAR_2436 MSMEG_3817 Mb1658c NC_000962.3 Mycobrowser_v4 CDS 199895 200935 . + 0 Rv0170 mce1B Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce1B Rv0170, (MTCI28.10), len: 346 aa. Mce1B (alternate gene name: mceD); belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07788|Rv0590|MTCY19H5.32c|mce2B (275 aa); O53968|Rv1967|MTV051.05|mce3B (342 aa); etc. Also highly similar to others e.g. NP_302657.1|NC_002677 putative secreted protein from Mycobacterium leprae (346 aa); CAC12797.1|AL445327 putative secreted protein from Streptomyces coelicolor (354 aa); etc. Contains hydrophobic region in N-terminal 30 residues. In Escherichia coli, N-terminal part is functional and directs export of a leaderless beta-lactamase into the periplasm (see Chubb et al., 1998). Predicted to be an outer membrane protein (See Song et al., 2008). O07414 No virulence, detoxification, adaptation O07414 O07414 O07414 ML2590 MMAR_0413 Mb0176 NC_000962.3 Mycobrowser_v4 CDS 213028 214140 . - 0 Rv0182c sigG The sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released. Probable alternative RNA polymerase sigma factor SigG (RNA polymerase ECF type sigma factor) Rv0182c, (MTCI28.22c), len: 370 aa (start site uncertain; first of several possibles was chosen, but note that this overlaps the upstream ORF). Probable sigG, alternative RNA polymerase sigma subunit (see citations below), similar to many e.g. Q45585|SIGW_BACSU RNA polymerase sigma factor from Bacillus subtilis (187 aa). Also similar to nine other ECF sigma factors from Mycobacterium tuberculosis e.g. Rv1221, Rv0735, etc. Contains PS01063 Sigma-70 factors ECF subfamily signature and probable helix-turn helix motif from aa 205-226 (Score 1181, +3.21 SD). Belongs to the sigma-70 factor family, ECF subfamily. P9WGG5 No information pathways O07426 P9WGG5 GO:0016987,GO:0003700,GO:0006352,GO:0006355 P9WGG5 ML2602,ML2602c MMAR_0426 MSMEG_0219 Mb0188c NC_000962.3 Mycobrowser_v4 CDS 382490 382876 . + 0 Rv0313 Rv0313 Function unknown Conserved protein Rv0313, (MTCY63.18), len: 128 aa. Conserved protein, equivalent only to CAC32049.1|AL583926 conserved hypothetical protein from Mycobacterium leprae (130 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WL03 No conserved hypotheticals P9WL03 ML2518,ML2518c MMAR_0564 MSMEG_3685 Mb0321 NC_000962.3 Mycobrowser_v4 CDS 438302 438739 . - 0 Rv0360c Rv0360c Function unknown Conserved protein Rv0360c, (MTCY13E10.22c), len: 145 aa. Conserved protein, equivalent to AL023514|MLCB4_16|CAA18948.1|AL023514|MLCB4.27c hypothetical protein from Mycobacterium leprae (137 aa), FASTA scores: opt: 793, E(): 0, (85.4% identity in 137 aa overlap). And similar to AL049754|SCH10_25c|T36537 hypothetical protein from Streptomyces coelicolor (143 aa), FASTA scores: opt: 497, E(): 3.2e-27, (55.8% identity in 138 aa overlap). O06310 No conserved hypotheticals O06310 O06310 ML0284,ML0284c MMAR_0672 MSMEG_0754 Mb0367c NC_000962.3 Mycobrowser_v4 CDS 985513 985971 . - 0 Rv0887c Rv0887c Function unknown Conserved hypothetical protein Rv0887c, (MTCY31.15c), len: 152 aa. Conserved hypothetical protein, highly similar to others e.g. NP_436346.1|NC_003037 Hypothetical protein from Sinorhizobium meliloti (149 aa); AL132644|SCI8_26 hypothetical protein from Streptomyces coelicolor (194 aa), FASTA scores: opt: 220, E(): 1.5e-07, (33.6% identity in 131 aa overlap); etc. Also shows weak similarity with transposases and related proteins. P9WKQ3 No conserved hypotheticals P64741 P9WKQ3 P9WKQ3 ML2133 MMAR_4645 MSMEG_5680 Mb0911c NC_000962.3 Mycobrowser_v4 CDS 202476 204068 . + 0 Rv0172 mce1D Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce1D Rv0172, (MTCI28.12), len: 530 aa. Mce1D; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07786|Rv0592|MTCY19H5.30c|mce2D (508 aa); O53970|Rv1969|MTV051.07|mce3D (423 aa); etc. Also highly similar to others e.g. NP_302659.1|NC_002677 putative secreted protein from Mycobacterium leprae (531 aa); CAC12795.1|AL445327 putative secreted protein from Streptomyces coelicolor (337 aa); etc. Hydrophobic region at N-terminus. Predicted to be an outer membrane protein (See Song et al., 2008). O07416 No virulence, detoxification, adaptation O07416 O07416 O07416 ML2592 MMAR_0415 MSMEG_0137 Mb0178 NC_000962.3 Mycobrowser_v4 CDS 204065 205237 . + 0 Rv0173 lprK Unknown, but thought to be involved in host cell invasion. Possible Mce-family lipoprotein LprK (Mce-family lipoprotein Mce1E) Rv0173, (MTCI28.13), len: 390 aa. Possible lprK (alternate gene name: mce1E), lipoprotein which belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07785|LPRL|Rv0593|MTCY19H5.29|mce2E (402 aa); O53971|LPRM|Rv1970|MTV051.08|mce3E (377 aa); etc. Also highly similar to others e.g. NP_302660.1|NC_002677 putative lipoprotein from Mycobacterium leprae (392 aa); CAC12794.1|AL445327 putative secreted protein from Streptomyces coelicolor (413 aa); etc. Contains PS00013 prokaryotic membrane lipoprotein lipid attachment site. O07417 No cell wall and cell processes O07417 O07417 O07417 ML2593 MMAR_0416 MSMEG_0138 Mb0179 NC_000962.3 Mycobrowser_v4 CDS 207452 208420 . + 0 Rv0176 Rv0176 Unknown Probable conserved Mce associated transmembrane protein Rv0176, (MTCI28.16), len: 322 aa. Probable conserved Mce-associated transmembrane protein. Contains short region of similarity to PRA_MYCLE|P41484 proline-rich antigen (36 kDa antigen) from Mycobacterium leprae (249 aa) (outside the proline-rich region), FASTA scores: opt: 165, E(): 2.9e-05, (40.0% identity in 65 aa overlap). Also similar to mce-associated proteins from Mycobacterium tuberculosis e.g. Rv1363c, Rv0177, Rv3493c, etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O07420 No cell wall and cell processes O07420 O07420 GO:0016021 ML2596 MMAR_0419 MSMEG_0141 Mb0182 NC_000962.3 Mycobrowser_v4 CDS 214088 214927 . + 0 Rv0183 Rv0183 Function unknown; probably involved in cellular metabolism. Possible lysophospholipase Rv0183, (MTCI28.23), len: 279 aa. Possible lysophospholipase, similar to several (especially eukaryotic enzymes, weaker with Escherichia coli), e.g. U67963|HSU67963_1 Human lysophospholipase homolog from Homo sapiens (313 aa), FASTA scores: opt: 569, E(): 2.6e-29, (37.1% identity in 259 aa overlap); P07000|PLDB_ECOLI lysophospholipase L2 from Escherichia coli (165 aa), FASTA scores: opt: 219, E(): 0.00012. Start changed based on similarity to AE001997_8 from Deinococcus radiodurans (282 aa), FASTA scores: opt: 510, E(): 1.4e-25, (34.8% identity in 282 aa overlap). Also shows some similarity to epoxide hydrolases from Mycobacterium tuberculosis e.g. Rv1938 FASTA score: (30.7% identity in 114 aa overlap); and O07214|YR15_MYCTU|Rv2715|MT2788|MTCY05A6.36 (341 aa). O07427 No intermediary metabolism and respiration O07427 GO:0016787 3.1.-.- O07427 ML2603 MSMEG_0220 Mb0189 NC_000962.3 Mycobrowser_v4 CDS 219486 219917 . + 0 Rv0188 Rv0188 Unknown Probable conserved transmembrane protein Rv0188, (MTCI28.27), len: 143 aa. Probable conserved transmembrane protein, similar to T35347|4835334|CAB42956.1|AL049863|SC5H1_31 probable membrane protein from Streptomyces coelicolor (147 aa), FASTA scores: opt: 326, E(): 6.5e-15, (36.2% identity in 141 aa overlap); N-terminus of P80185|MTRC_METTH tetrahydromethanopterin S-methyltransferase subunit C from Methanobacterium thermoautotrophicum strain Marburg/DSM 2133 (266 aa), FASTA scores: opt: 125, E(): 0.033, (31.6% identity in 98 aa overlap). Also similar to Rv3635 from Mycobacterium tuberculosis. O07432 No cell wall and cell processes O07432 O07432 GO:0016021 Mb0194 NC_000962.3 Mycobrowser_v4 CDS 286898 287071 . - 0 Rv0236A Rv0236A Unknown Small secreted protein Rv0236A, len: 57 aa. Small secreted protein. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P0A5C5 No cell wall and cell processes P0A5C5 P0A5C5 ML2569A MMAR_0497 MSMEG_0360 Mb0242c NC_000962.3 Mycobrowser_v4 CDS 206814 207455 . + 0 Rv0175 Rv0175 Unknown Probable conserved Mce associated membrane protein Rv0175, (MTCI28.15), len: 213 aa. Probable conserved Mce-associated membrane protein, equivalent, but longer in N-terminus, to CAC32127.1|AL583926 possible membrane protein from Mycobacterium leprae (182 aa). Also similar to mce-associated proteins from Mycobacterium tuberculosis e.g. Rv1363c, Rv0177, Rv1973, etc. Contains two 12 residue direct repeats at N-terminus. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O07419 No cell wall and cell processes O07419 O07419 ML2595 MMAR_0418 MSMEG_0140 Mb0181 NC_000962.3 Mycobrowser_v4 CDS 216269 218344 . + 0 Rv0186 bglS Possibly involved in degradation [catalytic activity: hydrolysis of terminal, non-reducing beta-D-glucose residues with release of beta-D-glucose]. Probable beta-glucosidase BglS (gentiobiase) (cellobiase) (beta-D-glucoside glucohydrolase) Rv0186, (MTCI28.25b), len: 691 aa. Probable bglS, beta-glucosidase, highly similar to many e.g. BGLS_AGRTU|P27034 beta-glucosidase from Agrobacterium tumefaciens (818 aa), FASTA scores: opt: 643, E(): 0, (32.5% identity in 842 aa overlap). Seems to belong to family 3 of glycosyl hydrolases. O07430 No intermediary metabolism and respiration O07430 O07430 GO:0005975,GO:0008422 3.2.1.21 O07430 ML2606 MMAR_0430 Mb0192 NC_000962.3 Mycobrowser_v4 CDS 218390 218551 . - 0 Rv0186A mymT Coordinates Cu(I) ions into a Cu(I)-thiolate core, protects cell from copper toxicity Metallothionein, MymT Rv0186A, len: 53 aa. MymT, metallothionein, equivalent to MAV_4993|A0QMH5 hypothetical protein from Mycobacterium avium (strain 104) (51 aa), and MAP_3626c|Q73TU2 hypothetical protein from Mycobacterium avium subsp. paratuberculosis (51 aa), FASTA scores: opt: 312, E(): 4.6e-17, (81.2% identity in 48 aa overlap). P9WK09 No virulence, detoxification, adaptation P9WK09 P9WK09 NC_000962.3 Mycobrowser_v4 CDS 218705 219367 . + 0 Rv0187 Rv0187 Thought to be involved in transfer of methyl group. Probable O-methyltransferase Rv0187, (MTCI28.26), len: 220 aa. Probable O-methyltransferase, similar to many e.g. AB93458.1|AL357591 putative O-methyltransferase from Streptomyces coelicolor (223 aa); MDMC_STRMY|Q00719 O-methyltransferase from Streptomyces mycarofaciens (221 aa), FASTA scores: opt: 327, E(): 2.4e-17, (35.9% identity in 192 aa overlap). Also similar to Rv1703c, Rv1220c from Mycobacterium tuberculosis. O07431 No intermediary metabolism and respiration O07431 O07431 GO:0008171 2.1.1.- O07431 MSMEG_0224 Mb0193 NC_000962.3 Mycobrowser_v4 CDS 265507 266295 . + 0 Rv0222 echA1 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Probable enoyl-CoA hydratase EchA1 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv0222, (MTCY08D5.17), len: 262 aa. Probable echA1, enoyl-CoA hydratase, similar to others e.g. AAC77915.1|AF063588 enoyl CoA hydratase from Rhodococcus fascians (275 aa); etc. P96404 No lipid metabolism P96404 P96404 GO:0016853,GO:0008152,GO:0004300 4.2.1.17 P96404 MMAR_0465 MSMEG_2227 NC_000962.3 Mycobrowser_v4 CDS 328575 329708 . - 0 Rv0272c Rv0272c Unknown Unknown protein Rv0272c, (MTCY06A4.16c), len: 377 aa. Unknown protein. P95229 No conserved hypotheticals P95229 ML2544 MMAR_0532 MSMEG_0605 Mb0278c NC_000962.3 Mycobrowser_v4 CDS 382879 383541 . - 0 Rv0314c Rv0314c Unknown Possible conserved membrane protein Rv0314c, (MTCY63.19c), len: 220 aa. Possible conserved membrane protein, with hydrophobic stretch from residues ~75-100. Similar in C-terminal part to Mycobacterium tuberculosis proteins Rv0679c and Rv0680c. O07241 No cell wall and cell processes O07241 O07241 ML2517 MMAR_0565 MSMEG_5317 Mb0322c NC_000962.3 Mycobrowser_v4 CDS 705961 706344 . - 0 Rv0611c Rv0611c Unknown Hypothetical protein Rv0611c, (MTCY19H5.10), len: 127 aa. Hypothetical unknown protein. Note that first start has been taken although this overlaps slightly with the upstream ORF. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6XVR9 No conserved hypotheticals I6XVR9 Mb0628c NC_000962.3 Mycobrowser_v4 CDS 221871 222161 . + 0 Rv0190 Rv0190 Function unknown Conserved protein Rv0190, (MTCI28.29), len: 96 aa. Conserved protein, highly similar to several hypothetical proteins e.g. SYCSLRA_35|Q55554|SLL0176 hypothetical 18.9 kDa protein from Synechocystis (167 aa), FASTA scores: opt: 237, E(): 5.8e-16, (39.4% identity in 94 aa overlap). Also highly similar to Z95398|MLCL622_7|O06070 from Mycobacterium leprae (135 aa), FASTA score: (82.6% identity in 92 aa overlap). Also similar to hypothetical proteins from Mycobacterium tuberculosis e.g. Rv0967, Rv0030, Rv1766 (42.5% identity in 80 aa overlap). O07434 No conserved hypotheticals O07434 O07434 O07434 ML2609 MMAR_0433 MSMEG_0230 Mb0196 NC_000962.3 Mycobrowser_v4 CDS 222289 223530 . + 0 Rv0191 Rv0191 Unknown; possibly involved in transport of drug across the membrane. Probable conserved integral membrane protein Rv0191, (MTCI28.30), len: 413 aa. Probable conserved integral membrane protein, member of major facilitator superfamily (MFS) possibly involved in transport of drug, similar to several hypothetical proteins e.g. YDEA_ECOLI|P31122 hypothetical 42.5 kd protein from Escherichia coli (396 aa), FASTA scores: opt: 475, E(): 4.2e-33, (29.7% identity in 381 aa overlap); and to several chloramphenicol resistance proteins e.g. CMLR_STRLI|P31141 chloramphenicol resistance protein from Streptomyces lividans (392 aa), FASTA scores: opt: 394, E(): 6.7e-12, (28.2% identity in 383 aa overlap). Also similar to SVU09991_1 from Mycobacterium tuberculosis. P9WJX7 No cell wall and cell processes O07435 P9WJX7 GO:0005886,GO:0055085,GO:0016021 ML2610 MMAR_0434 MSMEG_0232 Mb0197 NC_000962.3 Mycobrowser_v4 CDS 223564 224664 . + 0 Rv0192 Rv0192 Function unknown Conserved hypothetical protein Rv0192, (MTCI28.31), len: 366 aa. Conserved hypothetical protein. Has Gly- Arg-rich region followed by highly Pro-rich repetitive region near N-terminus. Similar in C-terminus to other hypothetical proteins e.g. Q49706|B1496_F2_81|U00013 from Mycobacterium leprae (271 aa), FASTA scores: opt: 375, E(): 3.2e-24, (36.1% identity in 255 aa overlap); YV09_MYCTU|Q11149|cY20G9.09 hypothetical 47.9 kDa protein from Mycobacterium tuberculosis (451 aa), FASTA scores: opt: 330, E(): 3.2e-13, (35.1% identity in 271 aa overlap). Also similar to Rv0116c, Rv1433, Rv2518c, Rv0483 from Mycobacterium tuberculosis. O07436 No conserved hypotheticals O07436 O07436 O07436 ML2611 MMAR_0435 MSMEG_0233 Mb0198 NC_000962.3 Mycobrowser_v4 CDS 226878 230462 . + 0 Rv0194 Rv0194 Thought to be involved in active transport of drugs across the membrane (export): multidrug resistance by an export mechanism. Responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane. Probable transmembrane multidrug efflux pump Rv0194, (MTV033.02), len: 1194 aa. Probable multidrug efflux pump (See Danilchanka et al., 2008), highly similar to many e.g. U62129|STU62129_2|T30293 ABC transport protein homolog from Salmonella typhi (1218 aa), FASTA scores: opt: 1116, E(): 0, (36.3% identity in 1209 aa overlap); CAB66302.1|AL136519 ABC transporter protein ATP-binding component from Streptomyces coelicolor (1243 aa); I84547 mdl protein from Escherichia coli (1143 aa); etc. Also similar to MTCY50_9 and MTCY50_10 from Mycobacterium tuberculosis, FASTA score: (33.8% identity in 574 aa overlap). Contains two PS00017 ATP/GTP-binding site motif A (P-loop) and one PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). Alternative start possible at 1823 but no RBS. O53645 No cell wall and cell processes O53645 O53645 GO:0042626,GO:0016021,GO:0055085,GO:0005524 O53645 MMAR_5223 Mb0200 NC_000962.3 Mycobrowser_v4 CDS 1078391 1078687 . + 0 Rv0968 Rv0968 Function unknown Conserved protein Rv0968, (MTCY10D7.06c), len: 98 aa. Conserved protein, part of cso operon, similar to NP_301579.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (92 aa). Also highly similar to conserved hypothetical proteins from Mycobacterium tuberculosis e.g. Rv3269 (93 aa), FASTA score: (51.1% identity in 94 aa overlap); and Rv1993c (90 aa). P9WKL9 No conserved hypotheticals P64779 P9WKL9 MMAR_1430,MMAR_2537,MMAR_4875 MSMEG_6059 Mb0993 NC_000962.3 Mycobrowser_v4 CDS 3841714 3842076 . - 0 Rv3424c Rv3424c Unknown Hypothetical protein Rv3424c, (MTCY78.05), len: 120 aa. Hypothetical unknown protein. P9WKY3 No conserved hypotheticals P9WKY3 Mb3458c NC_000962.3 Mycobrowser_v4 CDS 231647 232231 . + 0 Rv0196 Rv0196 Possibly involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0196, (MTV033.04), len: 194 aa. Possible transcriptional regulatory protein, similar to two Bacillus subtilis regulators: P42105|YXAF_BACSU hypothetical 21.0 kDa protein (191 aa), FASTA scores: opt: 323, E(): 2.1e-15, (30.9% identity in 181 aa overlap); and Z99105|BSUB0002_9 negative regulator of the lincomycin operon (188 aa), FASTA scores: opt: 255, E(): 1e-10, (25.9 identity in 185 aa overlap). P9WME1 No regulatory proteins O53647 P9WME1 GO:0006350,GO:0003700,GO:0006355 P9WME1 MMAR_0436 MSMEG_3520 Mb0202 NC_000962.3 Mycobrowser_v4 CDS 234516 236507 . - 0 Rv0198c zmp1 Function unknown; hydrolyzes peptides and/or proteins. Probable zinc metalloprotease Zmp1 Rv0198c, (MTV033.06c), len: 663 aa. Probable zmp1, zinc metalloprotease, equivalent to Z95398|MLCL622.12c from Mycobacterium leprae (667 aa), FASTA scores: opt: 3710, E(): 0, (80.8 % identity in 667 aa overlap). Also similar to many other metalloproteases e.g. members of the eukaryotic neprilysin family: P08473|NEP_HUMAN neprilysin (749 aa), FASTA scores: opt: 872, E(): 0, (31.1% identity in 692 aa overlap); Q07744|PEPO_LACLA neutral endopeptidase from Lactococcus lactis (626 aa), FASTA scores: opt: 862, E(): 0, (30.0% identity in 654 aa overlap). Contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. Belongs to peptidase family M13 (zinc metalloprotease); also known as the neprilysin subfamily. I6X8R2 No intermediary metabolism and respiration 3ZUK O53649 I6X8R2 GO:0006508,GO:0004222 3.4.24.- I6X8R2 ML2613,ML2613c MMAR_0438 MSMEG_0234 Mb0204c NC_000962.3 Mycobrowser_v4 CDS 236550 237209 . + 0 Rv0199 Rv0199 Unknown Probable conserved membrane protein Rv0199, (MTV033.07), len: 219 aa. Probable conserved membrane protein, equivalent to Z95398|MLCL622.13 from Mycobacterium leprae (224 aa), FASTA scores: opt: 920, E(): 0, (67.7% identity in 220 aa overlap). Also some similarity to Mce-associated membrane proteins from Mycobacterium tuberculosis e.g. Rv0178, Rv0175, etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53650 No cell wall and cell processes O53650 O53650 ML2614 MMAR_0439 MSMEG_0235 Mb0205 NC_000962.3 Mycobrowser_v4 CDS 248115 248906 . - 0 Rv0208c Rv0208c Causes methylation Hypothetical methlytransferase (methylase) Rv0208c, (MTCY08D5.03c), len: 263 aa. Hypothetical methyltransferase, equivalent to Z95398|MLCL622_20 from Mycobacterium leprae (279 aa), FASTA score: (64.2% identity in 246 aa overlaps). Also similar to others e.g. 10178368|CAC08407.1|AL392177|Q9F305|MT04_STRCO|SCD17A.03c hypothetical methlytransferase from Streptomyces coelicolor (271 aa). Could start at aa 7. P9WFY9 No intermediary metabolism and respiration P67498 P9WFY9 GO:0006400,GO:0008176 2.1.1.33 P9WFY9 ML2622,ML2622c MMAR_0448 MSMEG_0252 Mb0214c NC_000962.3 Mycobrowser_v4 CDS 254637 255950 . - 0 Rv0213c Rv0213c Causes methylation Possible methyltransferase (methylase) Rv0213c, (MTCY08D5.08c), len: 437 aa. Possible methyltransferase, weakly similar to others methyltransferases e.g. AF127374_30|LINA from Streptomyces lavendulae (611 aa), FASTA scores: opt: 400, E(): 8.1e-19, (27.3% identity in 388 aa overlap); Q50258 fortimicin kl1 methyltransferase (553 aa), FASTA scores: opt: 267, E(): 1.2e-13, (29.3% identity in 351 aa overlap). P96395 No intermediary metabolism and respiration P96395 P96395 GO:0009451,GO:0031419,GO:0046872,GO:0008168,GO:0051539 2.1.1.- P96395 MMAR_1693 Mb0219c NC_000962.3 Mycobrowser_v4 CDS 2204212 2204706 . + 0 Rv1961 Rv1961 Unknown Hypothetical protein Rv1961, MTCY09F9.03c, len: 164 aa. Hypothetical unknown protein. P95253 No conserved hypotheticals P95253 Mb1996 NC_000962.3 Mycobrowser_v4 CDS 241514 241924 . + 0 Rv0203 Rv0203 Unknown Possible exported protein Rv0203, (MTV033.11), len: 136 aa. Possible exported protein (has hydrophobic stretch near N-terminus). Some similarity to part of U02459|LDU02459_1 hypothetical protein from Leishmania donovani (741 aa), FASTA score: opt: 111, E(): 9.1, (30.0% identity in 90 aa overlap). I6X8R5 No cell wall and cell processes 3MAY I6X8R5 I6X8R5 MMAR_0443 MSMEG_0242 Mb0209 NC_000962.3 Mycobrowser_v4 CDS 241976 243214 . - 0 Rv0204c Rv0204c Unknown Probable conserved transmembrane protein Rv0204c, (MTV033.12c), len: 412 aa. Probable conserved transmembrane protein (see citation below), equivalent, but has C-terminal extension, to Z95398|MLCL622.17c from Mycobacterium leprae (367 aa), FASTA scores: opt: 2002, E(): 0, (82.4% identity in 374 aa overlap). Some similarity to Rv0585c from Mycobacterium tuberculosis. Nucleotide position 242299 in the genome sequence has been corrected, C:G resulting in V306L. I6Y748 No cell wall and cell processes I6Y748 GO:0016021 ML2618,ML2618c MMAR_0444 MSMEG_0248 Mb0210c NC_000962.3 Mycobrowser_v4 CDS 243384 244487 . + 0 Rv0205 Rv0205 Unknown Probable conserved transmembrane protein Rv0205, (MTV033.13), len: 367 aa. Possible conserved transmembrane protein, similar to hypothetical proteins from many bacteria e.g. AL0209|SC4H8_6 from Streptomyces coelicolor (402 aa), FASTA scores: opt: 436, E(): 1.7e-21, (27.2% identity in 349 aa overlap); Z99117|BSUB0014_221 from Bacillus subtilis (353 aa), FASTA scores: opt: 394, E(): 8.6e-19, (28.7% identity in 324 aa overla). P9WFM5 No cell wall and cell processes O53656 P9WFM5 GO:0005886,GO:0016021 P9WFM5 ML2619 MMAR_0445 MSMEG_0249 Mb0211 NC_000962.3 Mycobrowser_v4 CDS 244484 247318 . - 0 Rv0206c mmpL3 Unknown. Thought to be involved in fatty acid transport. Possible conserved transmembrane transport protein MmpL3 Rv0206c, (MTV033.14c, MTCY08D5.01c), len: 944 aa. Possible mmpL3, conserved transmembrane transport protein (see Tekaia et al., 1999), equivalent to Z95398|MLCL622.18c from Mycobacterium leprae (955 aa), FASTA scores: opt: 806, E(): 1.8e-21, (57.2% identity in 243 aa overlap). Member of RND superfamily, similar to others. Belongs to the MmpL family. P9WJV5 No cell wall and cell processes O53657 P9WJV5 GO:0005886,GO:0016021 ML2620,ML2620c MMAR_0446 MSMEG_0250 Mb0212c NC_000962.3 Mycobrowser_v4 CDS 247384 248112 . - 0 Rv0207c Rv0207c Function unknown Conserved hypothetical protein Rv0207c, (MTCY08D5.02c), len: 242 aa. Conserved hypothetical protein, equivalent to Z95398|MLCL622_19 from Mycobacterium leprae (261 aa), FASTA scores: E(): 0, (60.8 identity in 199 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P96389 No conserved hypotheticals P96389 P96389 ML2621,ML2621c MSMEG_0251 Mb0213c NC_000962.3 Mycobrowser_v4 CDS 1003957 1004484 . + 0 Rv0901 Rv0901 Function unknown Possible conserved exported or membrane protein Rv0901, (MTCY31.29), len: 175 aa. Possible conserved exported or membrane protein, with hydrophobic N-terminus at aa 7-25. Shows some similarity in C-terminus to O33070|Z99494|MLCB57.59 hypothetical protein from Mycobacterium leprae (113 aa), FASTA scores: opt: 204, E(): 3.2e-12, (44.9% identity in 78 aa overlap). P9WJG5 No cell wall and cell processes P9WJG5 ML2125,ML2125c MMAR_4635 Mb0925 NC_000962.3 Mycobrowser_v4 CDS 3195545 3196432 . - 0 Rv2886c Rv2886c Prevents the cointegration of foreign DNA before integration into the chromosome. Probable resolvase Rv2886c, (MTCY274.17c), len: 295 aa. Probable resolvase for IS1539. Contains PS00213 Lipocalin signature. P9WL35 No insertion seqs and phages P65045 P9WL35 GO:0006310,GO:0000150,GO:0003677 P9WL35 Mb2910c NC_000962.3 Mycobrowser_v4 CDS 253669 254640 . - 0 Rv0212c nadR Involved in transcriptional mechanism. Possible transcriptional regulatory protein NadR (probably AsnC-family) Rv0212c, (MTCY08D5.07c), len: 323 aa. Possible nadR (alternate gene name: nadI), transcriptional regulator, similar to others e.g. NADR_ECOLI|P27278 transcriptional regulator from Escherichia coli (410 aa), FASTA scores: opt: 377, E (): 1e-17, (31.1% identity in 347 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). P96394 No regulatory proteins P96394 GO:0003824,GO:0019363,GO:0045449,GO:0030528,GO:0006810,GO:0003677 P96394 MMAR_2965 Mb0218c NC_000962.3 Mycobrowser_v4 CDS 258913 259926 . + 0 Rv0216 Rv0216 Unknown Double hotdog hydratase Rv0216, (MTCY08D5.11), len: 337 aa. Double hotdog R-specific hydratase of unknown function, shows no activity for crotonyl-CoA, equivalent to Z95398|MLCL622_22 from Mycobacterium leprae (339 aa), FASTA scores: E(): 0, (73.7 identity in 338 aa overlap). Shows structural similarity to six others in Mycobacterium tuberculosis (see Castell et al (2005) below). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). I6Y340 No intermediary metabolism and respiration 2BI0 I6Y340 I6Y340 ML2627 MMAR_0456 MSMEG_0261 Mb0222 NC_000962.3 Mycobrowser_v4 CDS 259923 260831 . - 0 Rv0217c lipW Function unknown; lipolytic enzyme probably involved in cellular metabolism. Possible esterase LipW Rv0217c, (MTCY08D5.12c), len: 302 aa. Possible esterase, showing similarity with others e.g. EST_ACICA|P18773 esterase (303 aa), FASTA scores: opt: 320, E(): 3.2e-13, (29.2% identity in 274 aa overlap). P96399 No intermediary metabolism and respiration P96399 P96399 GO:0016787 3.1.1.- P96399 MSMEG_0280 Mb0223c NC_000962.3 Mycobrowser_v4 CDS 260924 262252 . + 0 Rv0218 Rv0218 Unknown Probable conserved transmembrane protein Rv0218, (MTCY08D5.13), len: 442 aa. Probable conserved transmembrane protein, some similarity with sulfite oxidases e.g. SUOX_HUMAN|P51687 sulfite oxidase precursor (488 aa), FASTA scores: opt: 153, E(): 0.0087, (28.6% identity in 161 aa overlap); and with some nitrate reductases e.g. NIA_FUSOX|P39863 nitrate reductase (NADPH) (905 aa), FASTA scores: opt: 143, E(): 0.06, (29.3% identity in 92 aa overlap). Also similar to BSUB0017_86 from Mycobacterium tuberculosis. P96400 No cell wall and cell processes P96400 P96400 GO:0016021,GO:0009055 P96400 MMAR_0461 MSMEG_0998 Mb0224 NC_000962.3 Mycobrowser_v4 CDS 387888 388550 . + 0 Rv0320 Rv0320 Unknown Possible conserved exported protein Rv0320, (MTCY63.25), len: 220 aa. Possible conserved exported protein, similar to some hypothetical proteins and to the middle part of a peptidase: NP_066789.1|10657900|AAG21739.1|AF116907 putative peptidase from Rhodococcus equi (546 aa). Also similar to Rv1728c|MTCY04C12.13c from Mycobacterium tuberculosis (256 aa), FASTA scores: opt: 497, E(): 1.2e-26, (41.8% identity in 225 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). O07246 No cell wall and cell processes O07246 O07246 ML2508,ML2508c MMAR_0599 MSMEG_0651 Mb0328 NC_000962.3 Mycobrowser_v4 CDS 264067 265476 . + 0 Rv0221 Rv0221 May be involved in synthesis of triacylglycerol Possible triacylglycerol synthase (diacylglycerol acyltransferase) Rv0221, (MTCY08D5.16), len: 469 aa. Possible triacylglycerol synthase (See Daniel et al., 2004), similar to other proteins from Mycobacterium tuberculosis e.g. Q50680|Rv2285|MT2343|MTCY339.25c 47.7 kDa protein (445 aa), FASTA scores: opt: 455, E(): 8.1e-23, (26.7% identity in 461 aa overlap); Rv3740c, Rv3734c, etc. P9WKB7 No lipid metabolism P96403 P9WKB7 GO:0006071,GO:0008610,GO:0004144 2.3.1.20 MMAR_0464 MSMEG_0290 Mb0227 NC_000962.3 Mycobrowser_v4 CDS 267863 268627 . - 0 Rv0224c Rv0224c Causes methylation Possible methyltransferase (methylase) Rv0224c, (MTCY08D5.19c), len: 254 aa. Possible methyltransferase, showing weak similarity with other methyltransferases e.g. P74388 sterol-C-methyltransferase (318 aa), FASTA scores: opt: 190, E(): 3.6e-05, (33.3% identity in 114 aa overlap). Equivalent to AL022486|MLCB1883_1 from Mycobacterium leprae (269 aa), FASTA scores: opt: 1456, E(): 0, (82.9% identity in 252 aa overlap). Also some similarity with MTCY21B4.22c from Mycobacterium tuberculosis FASTA score: (30.1% identity in 136 aa overlap). P9WJZ9 No intermediary metabolism and respiration P96406 P9WJZ9 GO:0008168,GO:0008152 2.1.1.- P9WJZ9 ML2584 MMAR_0473 Mb0229c NC_000962.3 Mycobrowser_v4 CDS 268663 269817 . + 0 Rv0225 Rv0225 Possibly involved in LPS biosynthesis. Possible conserved protein Rv0225, (MTCY08D5.20), len: 384 aa. Possible conserved protein involved in LPS biosynthesis, similar to O26275 LPS biosynthesis RFBU related protein (382 aa), FASTA scores: opt: 426, E(): 1.2e-20, (28.2% identity in 394 aa overlap). Some similarity with Rv3032 from Mycobacterium tuberculosis FASTA score: (31.6% identity in 228 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). P96407 No cell wall and cell processes P96407 P96407 GO:0016740,GO:0009058 P96407 ML2583,ML2583c MMAR_0474 MSMEG_0311 Mb0230 NC_000962.3 Mycobrowser_v4 CDS 296809 298119 . + 0 Rv0246 Rv0246 Unknown Probable conserved integral membrane protein Rv0246, (MTV034.12), len: 436 aa (start uncertain). Probable conserved integral membrane protein, similar to Rv2209|1237062|CAA94252.1|Z70283|Q10398|YM09_MYCTU from Mycobacterium tuberculosis (512 aa), FASTA scores: opt: 712, E(): 0, (33.2% identity in 422 aa overlap). O53668 No cell wall and cell processes O53668 MMAR_0508 Mb0252 NC_000962.3 Mycobrowser_v4 CDS 349935 351476 . + 0 Rv0286 PPE4 Function unknown PPE family protein PPE4 Rv0286, (MTV035.14), len: 513 aa. PPE4, Member of the Mycobacterium tuberculosis PPE family, similar to others e.g. AL0212|MTV012_32 from Mycobacterium tuberculosis (434 aa), FASTA scores: opt: 958, E(): 0, (43.5% identity in 522 aa overlap). P9WI43 No PE/PPE Q7DA35 P9WI43 GO:0005886,GO:0016021 P9WI43 ML2533,ML2533c MMAR_0545 MSMEG_0619 Mb0294 NC_000962.3 Mycobrowser_v4 CDS 554016 554309 . + 0 Rv0463 Rv0463 Unknown Probable conserved membrane protein Rv0463, (MTV038.07), len: 97 aa. Probable conserved transmembrane protein, highly similar to AAA63017.1|U15183 hypothetical protein from Mycobacterium leprae (101 aa), FASTA scores: opt: 364, E(): 4e-21, (57.9% identity in 95 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53748 No cell wall and cell processes O53748 ML2388 MMAR_0786 MSMEG_0904 Mb0472 NC_000962.3 Mycobrowser_v4 CDS 271574 272839 . - 0 Rv0227c Rv0227c Unknown Probable conserved membrane protein Rv0227c, (MTCY08D5.22c), len: 421 aa. Possible conserved membrane protein, equivalent to AL022486|MLCB1883_4 from Mycobacterium leprae (448 aa), FASTA scores: opt: 2148, E(): 0, (76.6% identity in 423 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P96409 No cell wall and cell processes P96409 P96409 P96409 ML2581 MMAR_0476 MSMEG_0317 Mb0232c NC_000962.3 Mycobrowser_v4 CDS 273055 274278 . + 0 Rv0228 Rv0228 Function unknown; probably involved in cellular metabolism. Probable integral membrane acyltransferase Rv0228, (MTCY08D5.23), len: 407 aa. Probable integral membrane acyltransferase, equivalent to 3063875|CAA18555.1|AL022486|T44870 acyltransferase from Mycobacterium leprae (384 aa), FASTA scores: opt: 2004, E(): 0, (79.3% identity in 381 aa overlap). Also similar to others e.g. Q11064 probable acyltransferase CY50.28C (383 aa), FASTA scores: opt: 372, E(): 2.6e-16, (35.9% identity in 359 aa overlap); Q00718|MDMB_STRMY acyltransferase. Very similar to Rv0111, Rv1254, etc from Mycobacterium tuberculosis. P96410 No intermediary metabolism and respiration P96410 P96410 GO:0016020,GO:0008415 2.3.1.- ML2580,ML2580c MMAR_0477 MSMEG_0319 Mb0233 NC_000962.3 Mycobrowser_v4 CDS 274306 274986 . - 0 Rv0229c Rv0229c Unknown Possible conserved membrane protein with PIN domain Rv0229c, (MTCY08D5.24c), len: 226 aa. Possible conserved membrane protein with PIN domain in C-terminal half, similar to several others from Mycobacterium tuberculosis. Has some similarity with Rv2757c|D70880 from Mycobacterium tuberculosis (138 aa). (See Arcus et al., 2005). FASTA scores: E(): 1e-15, (45.3% identity in 137 aa overlap), and Rv0301, Rv2546, etc. Also some similarity with Q48177 virulence associated protein C (132 aa), FASTA scores: opt: 101, E(): 0.6, (24.3% identity in 136 aa overlap). Contains PS00626 Regulator of chromosome condensation (RCC1) signature 2. L0T5V6 No cell wall and cell processes P96411 L0T5V6 L0T5V6 Mb0234c NC_000962.3 Mycobrowser_v4 CDS 274983 275963 . - 0 Rv0230c php Enzymatic activity unknown [catalytic activity: aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol]. Probable phosphotriesterase Php (parathion hydrolase) (PTE) (aryldialkylphosphatase) (paraoxonase) (a-esterase) (aryltriphosphatase) (paraoxon hydrolase) Rv0230c, (MTCY08D5.26c), len: 326 aa. Probable php, phosphotriesterase, similar to others e.g. AAK42653.1|AE006849 putative aryldialkylphosphatase (phosphotriesterase) (paraoxonase) from Sulfolobus solfataricus (314 aa); PHP_ECOLI|P45548 phosphotriesterase homology protein from Escherichia coli (292 aa), FASTA scores: opt: 408, E(): 7.1e-20, (31.1% identity in 305 aa overlap); OPD_FLASP|P16648 parathion hydrolase precursor (365 aa), FASTA scores: opt: 319, E(): 5.1e-14, (34.5% identity in 333 aa overlap); etc. Belongs to the phosphotriesterase family. Cofactor: contains 2 moles of zinc per subunit. P9WHN9 No lipid metabolism P96413 P9WHN9 GO:0016788,GO:0008270,GO:0009056 P9WHN9 ML2579 MMAR_0487 MSMEG_0320 Mb0235c NC_000962.3 Mycobrowser_v4 CDS 289104 289337 . + 0 Rv0239 vapB24 Unknown Possible antitoxin VapB24 Rv0239, (MTV034.05), len: 77 aa. Possible vapB24, antitoxin, part of toxin-antitoxin (TA) operon with Rv0240. Weakly similar to others e.g. Rv1839c|Z83859|MTCY359_34 from Mycobacterium tuberculosis (87 aa). See Arcus et al. 2005. FASTA scores: opt: 88, E(): 5, (40.0% identity in 45 aa overlap). P9WJ41 No virulence, detoxification, adaptation P9WJ41 Mb0245 NC_000962.3 Mycobrowser_v4 CDS 479789 480160 . + 0 Rv0401 Rv0401 Unknown Probable conserved transmembrane protein Rv0401, (MTCY04D9.14), len: 123 aa. Probable conserved transmembrane protein, equivalent to AL023514|MLCB4_9 putative integral membrane protein from Mycobacterium leprae (122 aa), FASTA scores: opt: 548, E(): 4.4e-32, (66.9% identity in 121 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P95210 No cell wall and cell processes P95210 P95210 GO:0016021 ML0271,ML0271c MMAR_0699 MSMEG_0245 Mb0407 NC_000962.3 Mycobrowser_v4 CDS 1241115 1241390 . - 0 Rv1116A Rv1116A Function unknown Conserved hypothetical protein (fragment) Rv1116A, len: 91 aa. Conserved hypothetical protein (possibly gene fragment), similar to C-terminal part of Rv1646|Z85982_9 from Mycobacterium tuberculosis (310 aa), FASTA scores: opt: 301, E(): 9.3e-13, (68.05% identity in 72 aa overlap). Also overlaps gene on other strand, Rv1116, at 3'-end. L7N6A9 No conserved hypotheticals L7N6A9 L7N6A9 Mb1147c NC_000962.3 Mycobrowser_v4 CDS 277899 278588 . + 0 Rv0232 Rv0232 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably TetR/AcrR-family) Rv0232, (MTCY08D5.28), len: 229 aa. Probable transcriptional regulatory protein, TetR/AcrR family, similar to others e.g. YIXD_BACSU|P32398 hypothetical transcriptional regulator (191 aa), FASTA scores: opt: 149, E(): 0.0014, (21.5% identity in 158 aa overlap). Also similar to MTV030_11 from Mycobacterium tuberculosis. Contains PS01081 Bacterial regulatory proteins, TetR family signature, and probable helix-turn helix motif from aa 33-54 (Score 1142, +3.08 SD). Belongs to the TetR/AcrR family of transcriptional regulators. P96415 No regulatory proteins P96415 P96415 GO:0006350,GO:0003700,GO:0006355 P96415 ML2575,ML2575c MMAR_0489 Mb0237 NC_000962.3 Mycobrowser_v4 CDS 278585 279529 . + 0 Rv0233 nrdB Involved in the DNA replication pathway (first reaction). Provides the precursors necessary for DNA synthesis [catalytic activity: 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O = ribonucleoside diphosphate + reduced thioredoxin]. Ribonucleoside-diphosphate reductase (beta chain) NrdB (ribonucleotide reductase small chain) Rv0233, (MTCY08D5.29), len: 314 aa. nrdB (alternate gene name: rnrS) ribonucleoside-diphosphate reductase, beta chain, similar to others e.g. RIR2_SCHPO|P36603 ribonucleoside-diphosphate reductase (391 aa), FASTA scores: opt: 168, E(): 0.00018, (26.1% identity in 199 aa overlap); etc. Belongs to the ribonucleoside diphosphate reductase small chain family. Cofactor: iron, manganese P9WH69 No information pathways 3EE4 P96416 P9WH69 GO:0055114,GO:0004748,GO:0046914,GO:0009186 1.-.-.- P9WH69 ML2574,ML2574c MSMEG_0358 Mb0238 NC_000962.3 Mycobrowser_v4 CDS 281166 282614 . - 0 Rv0235c Rv0235c Unknown Probable conserved transmembrane protein Rv0235c, (MTCY08D5.31c), len: 482 aa. Probable conserved transmembrane protein, highly similar to AL133278|CAB61913.1|SCM11_2 putative integral membrane protein from Streptomyces coelicolor (470 aa), FASTA scores: opt: 2116, E(): 0, (61.8% identity in 474 aa overlap); and similar to hypothetical proteins from other organisms e.g. Q13392|384D8_7 hypothetical protein (579 aa), FASTA scores: opt: 355, E(): 6.9e-17, (28.5% identity in 569 aa overlap). P96418 No cell wall and cell processes P96418 P96418 GO:0016021 ML2572 MMAR_0493 MSMEG_1761 Mb0240c NC_000962.3 Mycobrowser_v4 CDS 282649 286851 . - 0 Rv0236c aftD Involved in the biosynthesis of the mycobacterial cell wall arabinan Possible arabinofuranosyltransferase AftD Rv0236c, (MTV034.01c, MTV034.02c, MTCY08D5.32c), len: 1400 aa. Possible aftD, arabinofuranosyltransferase (See Skovierova et al., 2009). Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). Probable conserved transmembrane protein, equivalent to AL022486|CAC32102.1|MLCB1883_7 possible integral membrane protein from Mycobacterium leprae (1440 aa), FASTA scores: opt: 7491, E(): 0, (78.8% identity in 1397 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P96419 No cell wall and cell processes P96419 P96419 GO:0016021,GO:0007155 P96419 ML2570 MMAR_0496 MSMEG_0359 Mb0241c NC_000962.3 Mycobrowser_v4 CDS 305453 305809 . + 0 Rv0253 nirD Involved in nitrate assimilation (denitrification); required for activity of the reductase [catalytic activity: 3 NAD(P)H + nitrite = 3 NAD(P)+ + NH4OH + H2O.] Probable nitrite reductase [NAD(P)H] small subunit NirD Rv0253, (MTV034.19), len: 118 aa. Probable nirD, nitrite reductase [NAD(P)H] small subunit, similar to others e.g. P23675|NIRD_ECOLI|B3366|Z4727|ECS4217 from Escherichia coli strains K12 and O157:H7 (108 aa), FASTA scores: opt: 271, E():1.7e-12, (41.9% identity in 105 aa overlap). Associates with NIRB|Rv0252. O53675 No intermediary metabolism and respiration O53675 GO:0042128,GO:0008942,GO:0055114,GO:0051537 1.6.6.4 O53675 ML2554,ML2554c MMAR_0517 MSMEG_0428 Mb0259 NC_000962.3 Mycobrowser_v4 CDS 288428 289042 . + 0 Rv0238 Rv0238 Possibly involved in transcriptional mechanism. Possible transcriptional regulatory protein (probably TetR-family) Rv0238, (MTV034.04), len: 204 aa. Possible transcriptional regulatory protein, TetR family, equivalent to AL022486|MLCB1883_9|T44874 probable transcription regulator from Mycobacterium leprae (208 aa), FASTA scores: opt: 1029, E(): 0, (80.9% identity in 199 aa overlap). Also similar to others e.g. CAB77290.1|AL160312 putative TetR-family regulatory protein from Streptomyces coelicolor (240 aa). Also similar to Mycobacterium tuberculosis proteins Z95120|Rv3208 (228 aa), FASTA scores: opt: 266, E(): 8.3e-12, (28.1% identity in 196 aa overlap); and Rv1019 (197 aa). O53661 No regulatory proteins O53661 O53661 GO:0006350,GO:0003700,GO:0006355 O53661 ML2568,ML2568c MMAR_0500 MSMEG_0363 Mb0244 NC_000962.3 Mycobrowser_v4 CDS 289812 290654 . - 0 Rv0241c htdX Involved in fatty acid synthesis type II (fas-II) Probable 3-hydroxyacyl-thioester dehydratase HtdX Rv0241c, (MTV034.07c), len: 280 aa. Probable htdX, 3-hydroxyacyl-thioester dehydratase (See Gurvitz et al., 2009), highly similar to MLCB1883.17c|T44876063881|CAA18566.1|AL022486 hypothetical protein from Mycobacterium leprae (280 aa), FASTA scores: opt: 1564, E(): 0, (81.8% identity in 280 aa overlap); and CAC32097.1|AL583926 conserved hypothetical protein from Mycobacterium leprae (300 aa). Shows structural similarity to six others in Mycobacterium tuberculosis (see Castell et al (2005) below). Also similar to proteins from other organisms e.g. CAB77291.1|AL160312 putative dehydratase from Streptomyces coelicolor (291 aa); part of BAA92930.1|AB032743 fatty acid synthetase beta subunit from Pichia angusta (2060 aa). Predicted to be an outer membrane protein (See Song et al., 2008). O53664 No intermediary metabolism and respiration O53664 O53664 GO:0004312,GO:0005835,GO:0055114,GO:0016491,GO:0006633 O53664 ML2566 MMAR_0502 MSMEG_0371 Mb0247c NC_000962.3 Mycobrowser_v4 CDS 302866 305427 . + 0 Rv0252 nirB Involved in nitrate assimilation (denitrification) (at the second step). This enzyme is a FAD flavoprotein that also contains a siroheme and one 2FE-2S iron-sulfur center [catalytic activity: 3 NAD(P)H + nitrite = 3 NAD(P)+ + NH4OH + H2O.] Probable nitrite reductase [NAD(P)H] large subunit [FAD flavoprotein] NirB Rv0252, (MTV034.18), len: 853 aa. Probable nirB (alternate gene name: nasB), nitrite reductase [NAD(P)H] large subunit, flavoprotein containing siroheme and a 2FE-2S iron-sulfur centre. Highly similar to many others bacterial enzymes e.g. P08201|NIRB_ECOLI nitrite reductase (NAD(P)H) large subunit from Escherichia coli strain K12 (847 aa), FASTA scores: opt: 2775, E(): 0, (49.8% identity in 840 aa overlap); Q06458|NIRB_KLEPN nitrite reductase (NAD(P)H) large subunit (957 aa), FASTA scores: opt: 2902, E(): 0, (54.2% identity in 827 aa overlap). Contains PS00365 Nitrite and sulfite reductases iron-sulfur/siroheme-binding site. Homodimer which associates with NIRD|Rv0253. Cofactors: FAD; Iron; Siroheme. O53674 No intermediary metabolism and respiration O53674 O53674 GO:0020037,GO:0050660,GO:0050661,GO:0042128,GO:0008942,GO:0055114,GO:0051539 1.6.6.4 O53674 ML2555,ML2555c MMAR_0516 MSMEG_0427 Mb0258 NC_000962.3 Mycobrowser_v4 CDS 310774 311517 . - 0 Rv0259c Rv0259c Function unknown Conserved hypothetical protein Rv0259c, (MTCY06A4.03c), len: 247 aa. Conserved hypothetical protein, showing some similarity to Rv2393|Z81368|MTCY253_28 from Mycobacterium tuberculosis (281 aa), FASTA scores: E(): 9.5e-16, (33.6 % identity in 235 aa overlap). Also some similarity with CAC33938.1|AL589708 putative secreted protein from Streptomyces coelicolor (248 aa). P95216 No conserved hypotheticals P95216 P95216 GO:0016829,GO:0046872,GO:0009236 P95216 MMAR_0519 MSMEG_0431 Mb0265c NC_000962.3 Mycobrowser_v4 CDS 339364 340974 . + 0 Rv0280 PPE3 Function unknown PPE family protein PPE3 Rv0280, (MTV035.08), len: 536 aa. PPE3, Member of the Mycobacterium tuberculosis PPE family, similar to others e.g. Z80108|MTCY21B4_4|Rv0453 from Mycobacterium tuberculosis (539 aa), FASTA scores: opt: 1131, E(): 0, (51.7% identity in 540 aa overlap). P9WI45 No PE/PPE Q79FY8 P9WI45 P9WI45 MMAR_0538 Mb0288 NC_000962.3 Mycobrowser_v4 CDS 351525 351818 . + 0 Rv0287 esxG Function unknown ESAT-6 like protein EsxG (conserved protein TB9.8) Rv0287, (MTV035.15), len: 97 aa. EsxG, ESAT-6 like protein. PE-family related protein; distant member of the Mycobacterium tuberculosis PE family, similar to Rv3020c|AL0212|MTV012.34 (97 aa), FASTA scores: opt: 564, E(): 0, (91.8% identity in 97 aa overlap). Contains probable helix-turn-helix motif at aa 14-35 (Score 144, +4.11 SD). Seems to belong to the ESAT6 family (see Gey Van Pittius et al., 2001). Note that previously known as TB9.8. O53692 No cell wall and cell processes 2KG7 O53692 O53692 ML2532,ML2532c MMAR_0546,MMAR_3001 MSMEG_0620 Mb0295 NC_000962.3 Mycobrowser_v4 CDS 2544289 2544621 . - 0 Rv2269c Rv2269c Unknown Hypothetical protein Rv2269c, (MTCY339.41), len: 110 aa. Unknown protein; questionable ORF. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLF9 No conserved hypotheticals P9WLF9 P9WLF9 Mb2292c NC_000962.3 Mycobrowser_v4 CDS 292171 293493 . + 0 Rv0243 fadA2 Function unknown, but involved in lipid degradation [catalytic activity: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA]. Probable acetyl-CoA acyltransferase FadA2 (3-ketoacyl-CoA thiolase) (beta-ketothiolase) Rv0243, (MTV034.09), len: 440 aa. Probable fadA2, acetyl-CoA acyltransferase (3-acyl-CoA thiolase), equivalent, but shorter 17 aa, to AL022486|MLCB1883_14T44879 acetyltransferase from Mycobacterium leprae (457 aa), FASTA scores: opt: 250 7, E(): 0, (87.6% identity in 435 aa overlap). Also highly similar to many e.g. G83046|PA478 probable acyl-CoA thiolase from Pseudomonas aeruginosa (425 aa); AB77293.1|AL160312 putative ketoacyl CoA thiolase from Streptomyces coelicolor (428 aa); P76503|7449731|YFCY_ECOLI|D65007|B2342 probable 3-ketoacyl-CoA thiolase (acetyl-CoA acyltransferase) (beta-ketothiolase) from Escherichia coli strain K-12 (436 aa), FASTA scores: opt: 914, E(): 0, (38.2% identity in 434 aa overlap); P55084|ECHB_HUMAN mitochondrial trifunctonal enzyme (474 aa), FASTA scores: opt: 881, E(): 0, (37.7 identity in 451 aa overlap). Contains PS00099 Thiolases active site. Belongs to the thiolase family. O86361 No lipid metabolism O86361 O86361 GO:0008152,GO:0003988 2.3.1.16 O86361 ML2564,ML2564c MMAR_0504 MSMEG_0373 Mb0249 NC_000962.3 Mycobrowser_v4 CDS 305825 306349 . - 0 Rv0254c cobU Involved in cobalamin biosynthesis. Probable bifunctional cobalamin biosynthesis protein CobU: cobinamide kinase + cobinamide phosphate guanylyltransferase Rv0254c, (MTV034.20), len: 174 aa. Probable cobU, cobalamin biosynthesis protein including a cobinamide kinase and cobinamide phosphate guanylyltransferase. Highly similar to many e.g. Q05599|COBU_SALTY cobinamide kinase / cobinamide phosphate guanylyltransferase from Salmonella typhimurium (181 aa), FASTA scores: opt: 308, E(): 1.1e-14, (38.7% identity in 181 aa overlap); P46886|COBU_ECOLI|B1993|Z3153|ECS2788 Bifunctional cobalamin biosynthesis protein cobU from Escherichia coli strains K12 and O157:H7 (181 aa); part of AL096872|SC5F7_10 from Streptomyces coelicolor (397 aa), FASTA scores: opt: 445, E(): 3.6e-23, (46.0% identity in 176 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). O53676 No intermediary metabolism and respiration O53676 O53676 GO:0043753,GO:0051188,GO:0000166,GO:0043752 2.-.-.- O53676 ML1826,ML1826c MMAR_3251 MSMEG_4274 Mb0260c NC_000962.3 Mycobrowser_v4 CDS 1282306 1282893 . + 0 Rv1156 Rv1156 Function unknown Conserved protein Rv1156, (MTCI65.23), len: 195 aa. Conserved protein, highly similar to CAC32318.1|AL583944 conserved hypothetical protein from Streptomyces coelicolor (197 aa). O06554 No conserved hypotheticals O06554 O06554 GO:0003824,GO:0006284 O06554 ML1507,ML1507c MMAR_4296 MSMEG_5156 Mb1187 NC_000962.3 Mycobrowser_v4 CDS 298863 300803 . - 0 Rv0248c Rv0248c Involved in interconversion of fumarate and succinate (aerobic respiration) [catalytic activity: succinate + acceptor = fumarate + reduced acceptor]. Probable succinate dehydrogenase [iron-sulfur subunit] (succinic dehydrogenase) Rv0248c, (MTV034.14c), len: 646 aa. Probable succinate dehydrogenase, flavoprotein subunit, highly similar to flavoprotein subunit of various succinate dehydrogenases e.g. M88696|RIRSDHA_1 flavoprotein from Rickettsia prowazekii (596 aa), FASTA scores: opt: 651, E(): 0, (34.6 % identity in 598 aa overlap). Also similar to truncated U00022_17 flavoprotein from Mycobacterium leprae (401 aa), FASTA scores: opt: 677, E(): 0, (39.0% identity in 423 aa overlap). Note that succinate dehydrogenase forms generally part of an enzyme complex containing four subunits: a flavoprotein (Rv0248c ?), an iron-sulfur (Rv0247c ?), and two hydrophobic anchor proteins (Rv0249c ?). O53670 No intermediary metabolism and respiration O53670 O53670 GO:0055114,GO:0000104,GO:0009055 1.3.99.1 O53670 ML2559 MMAR_0511 MSMEG_0418 Mb0254c NC_000962.3 Mycobrowser_v4 CDS 300834 301655 . - 0 Rv0249c Rv0249c Could be involved in interconversion of fumarate and succinate (aerobic respiration). This hydrophobic component may be required to anchor the catalytic components of the succinate dehydrogenase complex to the cytoplasmic membrane. Probable succinate dehydrogenase [membrane anchor subunit] (succinic dehydrogenase) Rv0249c, (MTV034.15c), len: 273 aa. Probable succinate dehydrogenase, membrane-anchor subunit for succinate dehydrogenase encoded by Rv0247c and Rv0248c. Highly similar to AC44315.1|AL596043 putative integral membrane protein from Streptomyces coelicolor (278 aa). Note that succinate dehydrogenase forms generally part of an enzyme complex containing four subunits: a flavoprotein (Rv0248c ?), an iron-sulfur (Rv0247c ?), and two hydrophobic anchor proteins (Rv0249c ?). O53671 No intermediary metabolism and respiration O53671 ML2558 MMAR_0512 MSMEG_0419 Mb0255c NC_000962.3 Mycobrowser_v4 CDS 301735 302028 . - 0 Rv0250c Rv0250c Function unknown. Possibly down-regulated by HSPR|Rv0353. Conserved protein Rv0250c, (MTV034.16c), len: 97 aa. Conserved protein, equivalent to MLCB1883.27c|T44883|3063888|CAA18576.1|AL022486 hypothetical protein from Mycobacterium leprae (98 aa), FASTA scores: opt: 478, E(): 4.4e-28, (72.6% identity in 95 aa overlap). Also similar to C-terminus of AC44316.1|AL596043|SCBAC31E11.05c hypothetical protein from Streptomyces coelicolor (146 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53672 No conserved hypotheticals O53672 ML2557 MMAR_0513 MSMEG_0163,MSMEG_0420 Mb0256c NC_000962.3 Mycobrowser_v4 CDS 302173 302652 . - 0 Rv0251c hsp Thought to be involved in the initiation step of translation at high temperature. Bound to 30S ribosomal subunit. Possibly a molecular chaperone. Seems to be regulated positively by SIGE|Rv1221 and negatively by HSPR|Rv0353. Heat shock protein Hsp (heat-stress-induced ribosome-binding protein A) Rv0251c, (MTV034.17c), len: 159 aa. Hsp (alternate gene name: hsp20, hrpA, acr2), heat-stress-induced ribosome-binding protein A (see citations below). Highly similar to AAD39038.1|AF072875_1|AF072875 putative HSP20 from Mycobacterium smegmatis (145 aa), FASTA scores: opt: 479, E(): 2.3e-24, (59.9% identity in 157 aa overlap); and similar to many bacterial and eukaryotic hsp proteins e.g. P12811|HS2C_CHLRE chloroplast heat shock 22KD protein from chlamydomonas reinhardtii (157 aa), FASTA scores: opt: 184, E(): 1.2e-05, (32.4% identity in 142 aa overlap). Also similar to PCC6803 Spore protein sp21 from Synechocystis sp. (146 aa), FASTA scores: opt: 213, E(): 1.2e-07, (30.3 identity in 145 aa overlap). Also similar to P30223|14KD_MYCTU 14 KDA antigen (16 KDA antigen) 19K major membrane protein (HSP 16.3) from Mycobacterium tuberculosis (144 aa). Belongs to the small heat shock protein (HSP20) family. O53673 No virulence, detoxification, adaptation O53673 O53673 GO:0006950 O53673 ML2556 MMAR_0515 MSMEG_0424 Mb0257c NC_000962.3 Mycobrowser_v4 CDS 2274569 2275408 . - 0 Rv2028c Rv2028c Unknown Universal stress protein family protein Rv2028c, (MTV018.15c), len: 279 aa. Universal stress protein family protein, highly similar to many, contains IPR006016 UspA domain. P9WFD9 No virulence, detoxification, adaptation O53474 P9WFD9 GO:0006950 P9WFD9 MSMEG_5245 Mb2053c NC_000962.3 Mycobrowser_v4 CDS 1779314 1779724 . + 0 Rv1573 Rv1573 Unknown Probable PhiRv1 phage protein Rv1573, (MTCY336.31c), len: 136 aa. Probable phiRv1 phage protein (see citation below). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06617 No insertion seqs and phages O06617 Mb1599 NC_000962.3 Mycobrowser_v4 CDS 307877 309547 . - 0 Rv0256c PPE2 Function unknown PPE family protein PPE2 Rv0256c, (MTV034.22c), len: 556 aa. PPE2, Member of the M. tuberculosis PPE family, similar to many e.g. Rv0280, Rv0286, etc. Equivalent to Z98756|MLCB2492.30 from Mycobacterium leprae (572 aa), FASTA scores: opt: 1837, E(): 0, (62.9% identity in 461 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WI47 No PE/PPE Q79FZ4 P9WI47 P9WI47 ML1828,ML1828c MMAR_2944 Mb0262c NC_000962.3 Mycobrowser_v4 CDS 309699 310073 . + 0 Rv0257 Rv0257 Function unknown Conserved hypothetical protein Rv0257, len: 124 aa. Hypothetical protein, orthologue of ML1828A conserved hypothetical protein from Mycobacterium leprae. Replaced Rv0257c (older annotation). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). L7N694 No conserved hypotheticals L7N694 ML1828A Mb0263 NC_000962.3 Mycobrowser_v4 CDS 310294 310749 . - 0 Rv0258c Rv0258c Function unknown Conserved hypothetical protein Rv0258c, (MTCY06A4.02c), len: 151 aa (alternative start possible). Conserved hypothetical protein, showing some similarity to Rv1685c|MTCI125_6 from Mycobacterium tuberculosis (207 aa), FASTA scores: E(): 9.3e-07, (32.1% identity in 140 aa overlap). Also some similarity with AL049819|SCE7_13|T36295 probable transcription regulator from Streptomyces coelicolor (204 aa), FASTA scores: opt: 158, E(): 0.00052, (27.0% identity in 111 aa overlap). P95215 No conserved hypotheticals P95215 P95215 ML2553 MMAR_0518 Mb0264c NC_000962.3 Mycobrowser_v4 CDS 311514 312659 . - 0 Rv0260c Rv0260c Could be involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0260c, (MTCY0A4.04c), len: 381 aa. Possible two-component response regulator, highly similar to CAB72204.1|AL138851 putative transcriptional regulator from Streptomyces coelicolor (395 aa); and similar to O34394|D69851|YJJA conserved hypothetical protein from Bacillus subtilis (270 aa), FASTA scores: opt: 312, E(): 7.4e-14, (25.8% identity in 267 aa overlap). Also some similarity to regulatory proteins at C-terminal region e.g. CUTR_STRLI|Q03756 transcriptional regulatory protein (217 aa), FASTA scores: opt: 138, E(): 0.02, (30.6% identity in 111 aa overlap). P95217 No regulatory proteins P95217 P95217 GO:0005622,GO:0006355,GO:0033014,GO:0000156,GO:0000160,GO:0004852,GO:0003677 P95217 MMAR_0520 MSMEG_0432 Mb0266c NC_000962.3 Mycobrowser_v4 CDS 358945 359748 . - 0 Rv0295c Rv0295c Function unknown Conserved protein Rv0295c, (MTV035.23c), len: 267 aa. Conserved protein, showing weak similarity with CAC46877.1|AL591790 conserved hypothetical protein from Sinorhizobium meliloti (213 aa); and NP_104818.1|14023999|BAB50604.1|AP00300 Protein with weak similarity to NodH from Mesorhizobium loti (257 aa). Predicted to be an outer membrane protein (See Song et al., 2008). O53699 No conserved hypotheticals O53699 O53699 ML2526 MMAR_4255 MSMEG_0630 Mb0303c NC_000962.3 Mycobrowser_v4 CDS 314309 314854 . - 0 Rv0262c aac Confers resistance to aminoglycosides (gentamicin, tobramycin, dibekacin, netilmicin, and 6'-N-ethylnetilmicin). Aminoglycoside 2'-N-acetyltransferase Aac (Aac(2')-IC) Rv0262c, (MTCY06A4.06c), len: 181 aa. Aac, aminoglycoside 2'-N-acetyltransferase (aac(2')-IC) (see citation below), highly similar to NP_302635.1|NC_002677 aminoglycoside 2'-N-acetyltransferase from Mycobacterium leprae (182 aa); Q49157|AAC2_MYCFO|AAC aminoglycoside 2'-N-acetyltransferase from Mycobacterium fortuitum (195 aa), Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. FASTA scores: opt: 884, E(): 0, (69.1% identity in 181 aa overlap); and P94968|AAC2_MYCSM|AAC aminoglycoside 2'-N-acetyltransferase from Mycobacterium smegmatis (210 aa) (see also citation below). Also similar to Q52424|AAC2_PROST aminoglycoside 2'-N-acetyltransferase from Providencia stuartii (178 aa). Belongs to the AAC(2')-I family of acetyltransferases. Note that previously known as aac(2')-IC. P9WQG9 No virulence, detoxification, adaptation 1M44,1M4D,1M4G,1M4I P0A5N0 P9WQG9 GO:0008152,GO:0046677,GO:0008080 2.3.1.- P9WQG9 ML2551 MMAR_0522 MSMEG_0434 Mb0268c NC_000962.3 Mycobrowser_v4 CDS 314864 315766 . - 0 Rv0263c Rv0263c Function unknown Conserved hypothetical protein Rv0263c, (MTCY06A4.07c), len: 300 aa. Conserved hypothetical protein, equivalent to NP_302634.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (305 aa). Also similar to others e.g. AL121596|SC51A_21 hypothetical protein from Streptomyces coelicolor (285 aa), FASTA scores: opt: 714, E(): 0, (45.3% identity in 289 aa overlap); NP_233164.1|NC_002506 conserved hypothetical protein from Vibrio cholerae (309 aa); NP_406216.1|NC_003143 conserved hypothetical protein from Yersinia pestis (316 aa); YH30_HAEIN|P44298|hi1730 hypothetical protein from Haemophilus influenzae (309 aa), FASTA scores: opt: 430, E(): 3e-20, (29.6% identity in 284 aa overlap); etc. Also similar to carboxylases eg NP_415240.1|NC_000913|P75745|YBGK_ECOLI putative carboxylase from Escherichia coli strain K12 (310 aa), FASTA score: (34.6% identity in 286 aa overlap); NP_459698.1|NC_003197 putative carboxylase from Salmonella typhimurium (310 aa); and to middle part of NP_420636.1|NC_002696 urea amidolyase-related protein from Caulobacter crescentus (1207 aa). P95220 No conserved hypotheticals P95220 P95220 P95220 ML2550 MMAR_0523 MSMEG_0435 Mb0269c NC_000962.3 Mycobrowser_v4 CDS 315783 316415 . - 0 Rv0264c Rv0264c Function unknown Conserved hypothetical protein Rv0264c, (MTCY06A4.08c), len: 210 aa. Conserved hypothetical protein, equivalent to CAC32080.1|AL583926 conserved hypothetical protein from Mycobacterium leprae (222 aa). Also similar to others hypothetical proteins e.g. AL121596|SC51A_20 from Streptomyces coelicolor (252 aa), FASTA scores: opt: 420, E(): 2.7e-20, (41.7% identity in 204 aa overlap); P75744|YBGJ_ECOLI hypothetical 23.9 KD protein from Escherichia coli (218 aa), FASTA scores: E(): 2.1e-14, (35.7% identity in 182 aa overlap); YH31_HAEIN|P44299|hi173 hypothetical protein from Haemophilus influenzae (213 aa), FASTA scores: opt: 252, E(): 8.3e-10, (31.1% identity in 183 aa overlap). P95221 No conserved hypotheticals P95221 P95221 P95221 ML2549 MSMEG_0436 Mb0270c NC_000962.3 Mycobrowser_v4 CDS 322764 323273 . - 0 Rv0268c Rv0268c Unknown Hypothetical protein Rv0268c, (MTCY06A4.12c), len: 169 aa. Hypothetical unknown protein. P95225 No conserved hypotheticals P95225 P95225 Mb0274c NC_000962.3 Mycobrowser_v4 CDS 351848 352138 . + 0 Rv0288 esxH Function unknown. May be involved in virulence. Low molecular weight protein antigen 7 EsxH (10 kDa antigen) (CFP-7) (protein TB10.4) Rv0288, (MT0301, MTV035.16), len: 96 aa. EsxH, low molecular weight protein antigen 7 (10 kDa antigen) (CFP-7) (Protein TB10.4) (see citations below), ala-rich protein; member of mycobacterial protein family containing ESAT-6, very similar to MTV012_33 from Mycobacterium tuberculosis (96 aa), FASTA scores: opt: 566, E(): 0, (84.4% identity in 96 aa overlap). Alternative start codon possible position 351878 (see Rosenkrands et al., 2000). Belongs to the ESAT6 family (see Skjot et al., 2000; 2002; Gey Van Pittius et al., 2001). Note that previously known as cfp7 (alternate gene name: TB10.4). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted possible vaccine candidate (See Zvi et al., 2008). P9WNK3 No cell wall and cell processes 2KG7 P9WNK3 P9WNK3 ML2531,ML2531c MMAR_0547,MMAR_3000 MSMEG_0621 Mb0296 NC_000962.3 Mycobrowser_v4 CDS 1961291 1961788 . - 0 Rv1735c Rv1735c Function unknown Hypothetical membrane protein Rv1735c, (MTCY04C12.20c), len: 165 aa. Hypothetical membrane protein, similar to part of O58614|PH0884|AP000004 Hypothetical malic acid transport protein from Pyrococcus horikoshii (330 aa), FASTA scores: opt: 167, E(): 0.0003, (29.2% identity in 120 aa overlap). P9WLS5 No cell wall and cell processes P71993 P9WLS5 GO:0005886,GO:0055085,GO:0016021 P9WLS5 Mb1764c NC_000962.3 Mycobrowser_v4 CDS 317525 321154 . - 0 Rv0266c oplA Catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate [catalytic activity: ATP + 5-oxo-L-proline + 2 H2O = ADP + phosphate + L-glutamate]. Probable 5-oxoprolinase OplA (5-oxo-L-prolinase) (pyroglutamase) (5-OPASE) Rv0266c, (MTCY06A4.10c), len: 1209 aa. Probable oplA, 5-oxoprolinase, highly similar to others or to hypothetical proteins e.g. AAK24340.1|AE005906 hydantoinase/oxoprolinase from Caulobacter crescentus (1196 aa); NP_103129.1|14022305|BAB48915.1|AP002997 5-oxoprolinase from Mesorhizobium loti (1210 aa); CAC48426.1|AL603642 conserved hypothetical protein from Sinorhizobium meliloti (1205 aa); S77037|slr0697|1006579|BAA10729.1|D6400 hypothetical protein from Synechocystis sp. strain PCC 6803 (1252 aa), FASTA scores: opt: 2016, E(): 0, (51.4% identity in 1247 aa overlap); P97608|OPLA_RAT|T42756|11278797 5-oxoprolinase (5-oxo-L-prolinase) (pyroglutamase) (5-OPASE) from Rattus norvegicus (1288 aa); etc. Belongs to the oxoprolinase family. P95223 No intermediary metabolism and respiration P95223 P95223 GO:0017168 3.5.2.9 P95223 Mb0272c NC_000962.3 Mycobrowser_v4 CDS 323338 324531 . - 0 Rv0269c Rv0269c Function unknown Conserved hypothetical protein Rv0269c, (MTCY06A4.13c), len: 397 aa. Conserved hypothetical protein, highly similar to AL079355|SC4C6_19 hypothetical protein from Streptomyces coelicolor (341 aa), FASTA scores: opt: 1019, E(): 0, (46.5% identity in 344 aa overlap), and similar to other proteins e.g. CAC49016.1|AL603644 putative ATP-dependent DNA ligase protein from Sinorhizobium meliloti (636 aa); O34398 YKOU protein from Bacillus subtilis (611 aa), FASTA score: (27.2% identity in 283 aa overlap). Also similar to proteins from Mycobacterium tuberculosis e.g. Rv3062, Rv3731 (both DNA ligases), and Rv0938, Rv3730c. P95226 No conserved hypotheticals P95226 P95226 GO:0006269,GO:0003896 P95226 ML2547 MMAR_0527 MSMEG_0597 Mb0275c NC_000962.3 Mycobrowser_v4 CDS 324567 326249 . + 0 Rv0270 fadD2 Function unknown, but involved in lipid degradation. Probable fatty-acid-CoA ligase FadD2 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv0270, (MTCY06A4.14), len: 560 aa. Probable fadD2, fatty-acid-CoA synthetase, similar to many e.g. LCFA_ECOLI|P29212 long-chain-fatty-acid--CoA ligase from Escherichia coli (561 aa), FASTA scores: opt: 544, E(): 2.9e-26, (27.7% identity in 535 aa overlap). Also similar to others from Mycobacterium tuberculosis e.g. MTCY493_2, MTCY8D5_9, MTCY6G11_8, etc. Contains PS00455 Putative AMP-binding domain signature. P95227 No lipid metabolism P95227 P95227 GO:0008152,GO:0016874 6.2.1.- P95227 ML2546,ML2546c MMAR_0528 MSMEG_0599 Mb0276 NC_000962.3 Mycobrowser_v4 CDS 326266 328461 . - 0 Rv0271c fadE6 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE6 Rv0271c, (MTCY06A4.15c), len: 731 aa. Probable fadE6, acyl-CoA dehydrogenase, with C-terminal half similar to many e.g. ACDS_HUMAN|P16219 acyl-CoA dehydrogenase (short-chain) from Homo sapiens (412 aa), FASTA scores: opt: 339, E(): 1.3e-13, (28.1% identity in 288 aa overlap). P95228 No lipid metabolism P95228 P95228 GO:0003995,GO:0055114,GO:0050660 1.3.99.- P95228 ML2545 MMAR_0530 MSMEG_0603 Mb0277c NC_000962.3 Mycobrowser_v4 CDS 379172 380401 . + 0 Rv0311 Rv0311 Unknown Unknown protein Rv0311, (MTCY63.16), len: 409 aa. Unknown protein. Contains PS00881 Protein splicing signature. O07238 No conserved hypotheticals O07238 O07238 ML2520,ML2520c MMAR_0561 MSMEG_5855 Mb0319 NC_000962.3 Mycobrowser_v4 CDS 392039 392263 . + 0 Rv0325 Rv0325 Unknown Hypothetical protein Rv0325, (MTCY63.30), len: 74 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07250 No unknown O07250 Mb0333 NC_000962.3 Mycobrowser_v4 CDS 2123684 2124370 . + 0 Rv1874 Rv1874 Unknown Unknown protein Rv1874, (MTCY180.44c), len: 228 aa. Unknown protein. O07755 No conserved hypotheticals O07755 ML2040,ML2040c MMAR_2757 MSMEG_3569 Mb1905 NC_000962.3 Mycobrowser_v4 CDS 330933 331658 . - 0 Rv0275c Rv0275c Could be involved in transcriptional mechanism. Possible transcriptional regulatory protein (possibly TetR-family) Rv0275c, (MTV035.03c), len: 241 aa. Possible transcriptional regulator, TetR family, similar to others e.g. Q9RJE7|SCF81.04c putative TetR-family transcriptional regulator from Streptomyces coelicolor (219 aa); Q9FBI8|SCP8.33c putative TetR-family transcriptional regulator from Streptomyces coelicolor (213 aa); Q9I2Q9|PA1836 probable transcriptional regulator from Pseudomonas aeruginosa (193 aa); etc. Also shows some similarity with Rv0825c from Mycobacterium tuberculosis (213 aa), FASTA scores: opt: 230, E(): 2.7e-07, (32.6% identity in 190 aa overlap). Seems to belong to the TetR/AcrR family of transcriptional regulators (M. tuberculosis regulatory protein family with many TetR orthologues). L7N6A2 No regulatory proteins Q7DA42 L7N6A2 GO:0006350,GO:0003700,GO:0006355 L7N6A2 ML2541 MMAR_0536 MSMEG_0612 Mb0281c NC_000962.3 Mycobrowser_v4 CDS 331748 332668 . + 0 Rv0276 Rv0276 Function unknown Conserved hypothetical protein Rv0276, (MTV035.04), len: 306 aa. Conserved hypothetical protein, similar to Rv2237|Z70692|MTCY427.18 from Mycobacterium tuberculosis (296 aa), FASTA scores: opt: 874, E(): 0, (49.6% identity in 282 aa overlap). O53682 No conserved hypotheticals O53682 O53682 O53682 ML2540,ML2540c MMAR_0537 MSMEG_0613 Mb0282 NC_000962.3 Mycobrowser_v4 CDS 332708 333136 . - 0 Rv0277c vapC25 Unknown Possible toxin VapC25. Contains PIN domain. Rv0277c, (MTV035.05c), len: 142 aa. Possible vapC25, toxin, part of toxin-antitoxin (TA) operon with Rv0277A, contains PIN domain, see Arcus et al. 2005. Highly similar to others e.g. Rv0749|H70824|2911023|CAA17516.1|AL021958 conserved hypothetical protein from Mycobacterium tuberculosis (142 aa); and Rv2530c, etc. P9WF85 No virulence, detoxification, adaptation O53683 P9WF85 P9WF85 Mb0283c NC_000962.3 Mycobrowser_v4 CDS 336560 339073 . - 0 Rv0279c PE_PGRS4 Function unknown PE-PGRS family protein PE_PGRS4 Rv0279c, (MTV035.07c), len: 837 aa. PE_PGRS4, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), similar to many e.g. Z95890|MTCY28_25|Rv0278c from Mycobacterium tuberculosis (914 aa), FASTA scores: opt: 2677, E(): 0, (64.5% identity in 926 aa overlap). L0T4W6 No PE/PPE Q79FY9 L0T4W6 L0T4W6 Mb0287c NC_000962.3 Mycobrowser_v4 CDS 345635 349627 . + 0 Rv0284 eccC3 Unknown ESX conserved component EccC3. ESX-3 type VII secretion system protein. Possible membrane protein. Rv0284, (MTV035.12), len: 1330 aa. eccC3, esx conserved component, ESX-3 type VII secretion system protein, possible membrane protein, similar to products of two adjacent Mycobacterium leprae genes, MLCB628.16c (744 aa) and MLCB628.15c (597 aa); and throughout its length to several large Mycobacterium tuberculosis proteins: Rv3447c, Rv3870, Rv1784, etc. Y14967|MLCB628_ 15 (744 aa), FASTA scores: opt: 942, E(): 0, (33.8% identity in 730 aa overlap); Y14967|MLCB628_14 (597 aa), FASTA scores: opt: 613, E(): 3.1e-30, (31.7% identity in 615 aa overlap); Z94121|MTY15F10_17 (1396 aa), FASTA scores: opt: 652, E(): 2.2e-32, (35.4% identity in 1321 aa overlap); Z95389|MTCY77_19 (1236 aa), FASTA scores: opt 652, E(): 2.2e-32, (35.4% identity in 1321 aa overlap). Contains three PS00017 ATP/GTP-binding site motif A (P-loop). P9WNA9 No cell wall and cell processes O53689 P9WNA9 GO:0003677,GO:0007049,GO:0051301,GO:0007059,GO:0016021,GO:0017111,GO:0005886,GO:0005524 P9WNA9 ML2535c MMAR_0543 MSMEG_0617 Mb0292 NC_000962.3 Mycobrowser_v4 CDS 344022 345638 . + 0 Rv0283 eccB3 Unknown ESX conserved component EccB3. ESX-3 type VII secretion system protein. Possible membrane protein. Rv0283, (MTV035.11), len: 538 aa. eccB3, esx conserved component, ESX-3 type VII secretion system protein, possible membrane protein, similar to several hypothetical mycobacterial proteins e.g. Z94121|MTY15F10_16|Rv3895c from Mycobacterium tuberculosis (495 aa), FASTA scores: opt: 698, E(): 0, (37.6% identity in 492 aa overlap); Rv1782; Rv3450c; Rv3869; and Y14967|MLCB628_16|MLCB628.17c from Mycobacterium leprae (481 aa), FASTA scores: opt: 672, E(): 1.5e-31, (37.2% identity in 506 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WNR3 No cell wall and cell processes O53688 P9WNR3 GO:0005886,GO:0016021 P9WNR3 ML2536c MMAR_0542 MSMEG_0616 Mb0291 NC_000962.3 Mycobrowser_v4 CDS 353083 354501 . + 0 Rv0290 eccD3 Unknown ESX conserved component EccD3. ESX-3 type VII secretion system protein. Probable transmembrane protein. Rv0290, (MTV035.18), len: 472 aa. EccD3, esx conserved component, ESX-3 type VII secretion system protein, probable transmembrane protein, similar to several others in mycobacteria e.g. Z95389|MTCY77_20|Rv3887c from Mycobacterium tuberculosis (467 aa), FASTA scores: opt: 429, E(): 5.1e-19, (28. 6% identity in 479 aa overlap); Rv3877; Rv1795; Rv3448; and Y14967|MLCB628_9|MLCB628.10c from Mycobacterium leprae (480 aa), FASTA scores: opt: 269, E(): 3.1e-09, (26.0% identity in 503 aa overlap). P9WNQ3 No cell wall and cell processes O86362 P9WNQ3 GO:0005886,GO:0016021 P9WNQ3 ML2529c MMAR_0549 MSMEG_0623 Mb0298 NC_000962.3 Mycobrowser_v4 CDS 384535 385149 . + 0 Rv0316 Rv0316 Could be involved in the catabolism of catechol to succinate- and acetyl-CoA in the beta-ketoadipate pathway (at the third step) [catalytic activity: 2,5-dihydro-5-oxofuran-2-acetate = 3,4-dihydro-5-oxofuran-2-acetate]. Possible muconolactone isomerase Rv0316, (MTCY63.21), len: 204 aa. Possible muconolactone isomerase, showing weak similarity with some muconolactone isomerases e.g. O33947|CTC1_ACILW muconolactone delta-isomerase 1 (MIASE 1)(96 aa), FASTA scores: opt: 179, E(): 3.9e-05, (32.6% identity in 92 aa overlap). O07243 No intermediary metabolism and respiration O07243 O07243 GO:0016853,GO:0006725 5.3.3.- O07243 MMAR_0568 Mb0324 NC_000962.3 Mycobrowser_v4 CDS 398254 398628 . + 0 Rv0333 Rv0333 Unknown Unknown protein Rv0333, (MTCY63.38), len: 124 aa. Unknown protein. O33273 No conserved hypotheticals O33273 O33273 ML2504,ML2504c MMAR_0605 MSMEG_0683 Mb0340 NC_000962.3 Mycobrowser_v4 CDS 3315236 3316456 . + 0 Rv2963 Rv2963 Unknown Probable integral membrane protein Rv2963, (MTCY349.24c), len: 406 aa. Probable integral membrane protein. I6YET7 No cell wall and cell processes P95129 I6YET7 ML1662 MMAR_1754 Mb2987 NC_000962.3 Mycobrowser_v4 CDS 355880 356875 . + 0 Rv0292 eccE3 Unknown ESX conserved component EccE3. ESX-3 type VII secretion system protein. Probable transmembrane protein. Rv0292, (MTV035.20), len: 331 aa. EccE3, esx conserved component, ESX-3 type VII secretion system protein, probable transmembrane protein (has two hydrophobic segments at N-terminal end), equivalent to CAC32058.1|AL583926 conserved membrane protein from Mycobacterium leprae (339 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WJE5 No cell wall and cell processes O53696 P9WJE5 GO:0005886,GO:0016021 ML2527c MMAR_0551 MSMEG_0626 Mb0300 NC_000962.3 Mycobrowser_v4 CDS 356862 358064 . - 0 Rv0293c Rv0293c Function unknown Conserved protein Rv0293c, (MTV035.21c), len: 400 aa. Conserved protein, similar in C-terminal part to Rv2627c|B70573|MTCY01A10.05|CAB08637.1|Z95387 conserved hypothetical protein from Mycobacterium tuberculosis (413 aa), FASTA scores: opt: 394, E(): 2.1e-17, (31.1% identity in 299 aa overlap). O53697 No conserved hypotheticals O53697 O53697 O53697 MMAR_0552 MSMEG_0625 Mb0301c NC_000962.3 Mycobrowser_v4 CDS 358171 358956 . + 0 Rv0294 tam Possibly catalyzes the S-adenosylmethionine monomethyl esterification of trans-aconitate at high affinity and of cis-aconitate, isocitrate, and citrate at lower velocities and affinities. Probable trans-aconitate methyltransferase Tam Rv0294, (MTV035.22), len: 261 aa. Probable tam, trans-aconitate methyltransferase, similar to others e.g. P76145|TAM_ECOLI|7465793|B64906|B1519 trans-aconitate methyltransferase from Escherichia coli strain K12 (252 aa), FASTA scores: opt: 649, E(): 0, (39.3 identity in 252 aa overlap). Belongs to the methyltransferase superfamily. P9WGA3 No intermediary metabolism and respiration P66885 P9WGA3 GO:0008152,GO:0030798,GO:0005737 2.1.1.144 P9WGA3 MMAR_0553 MSMEG_0629 Mb0302 NC_000962.3 Mycobrowser_v4 CDS 442395 443078 . + 0 Rv0364 Rv0364 Unknown Possible conserved transmembrane protein Rv0364, (MTCY13E10.26), len: 227 aa. Possible conserved transmembrane protein, equivalent to O69601|Y364_MYCLE|ML0287|CAA18951.1|AL023514|AL023514|MLCB4_19 hypothetical 24.3 KDA protein from Mycobacterium leprae (222 aa), FASTA scores: opt: 1027, E(): 0, (66.1% identity in 227 aa overlap). Shows strong similarity to DEDA_ECOLI|P09548 DedA protein protein from Escherichia coli FASTA scores: E(): 1.3e-28, (39.5% identity in 195 aa overlap). Similar also to Mycobacterium tuberculosis DedA protein Rv2637|MTCY441.0. P9WP09 No cell wall and cell processes O06314 P9WP09 GO:0005886,GO:0016021 ML0287 MMAR_0668 Mb0371 NC_000962.3 Mycobrowser_v4 CDS 600098 600391 . + 0 Rv0508 Rv0508 Function unknown Conserved hypothetical protein Rv0508, (MTCY20G9.35), len: 97 aa. Conserved hypothetical protein, showing similarity with T36269|5123666|CAB45355.1|AL079345 probable redoxin from Streptomyces coelicolor (101 aa), FASTA scores: opt: 160, E(): 3.4e-05, (33.3% identity in 75 aa overlap); and E81943|NMA0966 probable thioredoxin from Neisseria meningitidis group a strain Z2491 (77 aa). P9WKS9 No conserved hypotheticals P64727 P9WKS9 ML2423,ML2423c MMAR_0841 MSMEG_0951 Mb0520 NC_000962.3 Mycobrowser_v4 CDS 365234 366142 . + 0 Rv0303 Rv0303 Function unknown; probably involved in cellular metabolism. Probable dehydrogenase/reductase Rv0303, (MTCY63.08), len: 302 aa. Possible dehydrogenase/reductase, similar to various NADPH dehydrogenases and other NADPH oxidoreductases e.g. O48741|PORC_ARATH|7488284|T00897 protochlorophyllide reductase C chloroplast precursor (NADPH-protochlorophyllide oxidoreductase C) from Arabidopsis thaliana (401 aa); Q42850 NADPH dehydrogenase (395 aa), FASTA scores: opt: 347, E(): 3.8e-16, (35.4% identity in 319 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07230 No intermediary metabolism and respiration O07230 O07230 GO:0055114,GO:0016491 1.-.-.- O07230 Mb0311 NC_000962.3 Mycobrowser_v4 CDS 363252 363479 . + 0 Rv0298 Rv0298 Unknown Hypothetical protein Rv0298, (MTCY63.03), len: 75 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WJ09 No conserved hypotheticals O07225 P9WJ09 GO:0006355,GO:0003677 Mb0306 NC_000962.3 Mycobrowser_v4 CDS 363476 363778 . + 0 Rv0299 Rv0299 Unknown Hypothetical protein Rv0299, (MTCY63.04), len: 100 aa. Hypothetical unknown protein. Equivalent to AAK44536.1 from Mycobacterium tuberculosis strain CDC1551 (49 aa) but longer 51 aa. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07226 No conserved hypotheticals O07226 Mb0307 NC_000962.3 Mycobrowser_v4 CDS 363826 364047 . + 0 Rv0300 vapB2 Unknown Possible antitoxin VapB2 Rv0300, (MTCY63.05), len: 73 aa. Possible vapB2, antitoxin, part of toxin-antitoxin (TA) operon with Rv0301 (See Arcus et al., 2005; Pandey and Gerdes, 2005). Weak similarity with others e.g. Rv3697c from Mycobacterium tuberculosis (145 aa), Rv1721c|MTCY04C12.06c|Z81360|MTCY4C12_4 conserved hypothetical protein from Mycobacterium tuberculosis (75 aa), FASTA scores: opt: 84, E(): 8.3, (39.5% identity in 38 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07227 No virulence, detoxification, adaptation 3H87 O07227 O07227 GO:0006355,GO:0003677 O07227 Mb0308 NC_000962.3 Mycobrowser_v4 CDS 392273 392728 . + 0 Rv0326 Rv0326 Unknown Hypothetical protein Rv0326, (MTCY63.31), len: 151 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07251 No unknown O07251 O07251 GO:0008168,GO:0008152 O07251 Mb0333 NC_000962.3 Mycobrowser_v4 CDS 397442 398227 . + 0 Rv0332 Rv0332 Function unknown Conserved protein Rv0332, (MTCY63.37), len: 261 aa. Conserved protein, similar to several conserved hypothetical proteins from Streptomyces coelicolor e.g. SC6A9.18c|AL031035|SC6A9_18|T35449 hypothetical protein (266 aa), FASTA scores: opt: 508, E(): 5.7e-27, (36.7% identity in 251 aa overlap). O07256 No conserved hypotheticals O07256 O07256 O07256 ML2505,ML2505c MMAR_0604 MSMEG_0682 Mb0339 NC_000962.3 Mycobrowser_v4 CDS 417304 418290 . + 0 Rv0347 Rv0347 Unknown Probable conserved membrane protein Rv0347, (MTCY13E10.07), len: 328 aa (alternative start possible). Probable conserved membrane protein, similar to Rv0831c|AL022004|MTV043_23 from Mycobacterium tuberculosis (271 aa), FASTA scores: E(): 9.6e-21, (33.1% identity in 266 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06298 No cell wall and cell processes O06298 O06298 Mb0355 NC_000962.3 Mycobrowser_v4 CDS 377113 377829 . + 0 Rv0308 Rv0308 Unknown Probable conserved integral membrane protein Rv0308, (MTCY63.13), len: 238 aa. Probable conserved integral membrane protein, with C-terminus highly similar to C-terminus of other integral membrane proteins or phosphatases e.g. AAK25788.1|AF336822_1|13430250|AAK25789.1|AF336823_1 putative phosphatase from Streptococcus pyogenes (201 aa); Q06074 hypothetical 24.9 kDa protein (216 aa), FASTA scores: opt: 209, E(): 2e-07, (27.9% identity in 140 aa overlap). Could be a phosphatase. O07235 No cell wall and cell processes O07235 O07235 GO:0016020,GO:0003824 O07235 ML2523,ML2523c MMAR_0558 MSMEG_0634 Mb0316 NC_000962.3 Mycobrowser_v4 CDS 377931 378587 . + 0 Rv0309 Rv0309 Unknown Possible conserved exported protein Rv0309, (MTCY63.14), len: 218 aa. Possible conserved exported protein (has putative N-terminal signal sequence), equivalent to AC32053.1|AL583926 putative secreted protein from Mycobacterium leprae (218 aa). Also similar to others e.g. AB76092.1|AL157956 putative secreted protein from Streptomyces coelicolor (238 aa). Predicted to be an outer membrane protein (See Song et al., 2008). O07236 No cell wall and cell processes O07236 O07236 O07236 ML2522,ML2522c MMAR_0559 MSMEG_0635 Mb0317 NC_000962.3 Mycobrowser_v4 CDS 378657 379148 . - 0 Rv0310c Rv0310c Function unknown Conserved protein Rv0310c, (MTCY63.15c), len: 163 aa. Conserved protein, similar to some bile acid dehydratases e.g. P19412|BAIE_EUBSP|98749|D37844|1381566|AAC45413.1|U57489 bile acid-inducible operon protein E from Eubacterium sp (166 aa), FASTA scores: opt: 302, E(): 1e-11, (38.8% identity in 134 aa overlap); AAF22847.1|AF210152_4 bile acid 7a-dehydratase from Clostridium sp. (168 aa). O07237 No conserved hypotheticals O07237 O07237 ML2521 MMAR_0560 MSMEG_0636 Mb0318c NC_000962.3 Mycobrowser_v4 CDS 380556 382418 . + 0 Rv0312 Rv0312 Function unknown Conserved hypothetical proline and threonine rich protein Rv0312, (MTCY63.17), len: 620 aa. Conserved hypothetical protein with highly Pro-, Thr-rich C-terminus. Similar to Pro-,Thr-rich region in Rv2264c|AL021925|MTV022_14 from Mycobacterium tuberculosis (592 aa), FASTA scores: opt: 1075, E(): 0, (38.9% identity in 627 aa overlap). Also some similarity with Rv0350|dnaK from Mycobacterium tuberculosis. Possibly membrane protein; has hydrophobic stetch in its middle part. O07239 No conserved hypotheticals O07239 O07239 GO:0005524 O07239 ML2519,ML2519c MMAR_0562 MSMEG_0638 Mb0320 NC_000962.3 Mycobrowser_v4 CDS 383602 384486 . + 0 Rv0315 Rv0315 Possibly hydrolyzes specific sugar (hydrolyzation of glycosidic bond) and could be involved in exopolysaccharide biosynthesis/degradation. Could also have a LYTIC activity against cell WALLS. Possible beta-1,3-glucanase precursor Rv0315, (MTCY63.20), len: 294 aa. Possible beta-1,3-glucanase precursor (has hydrophobic stretch in its N-terminal part), similar to others e.g. Q51333|AAC44371.1 beta-1,3-glucanase II a from Oerskovia xanthineolytica (306 aa), FASTA scores: opt: 76, E(): 3e-14, (34.1% identity in 302 aa overlap); and AAC38290.1|AF052745 beta-1,3-glucanase II from Oerskovia xanthineolytica (435 aa). Contains glycosyl hydrolases family 16 active site signature (PS01034). O07242 No intermediary metabolism and respiration O07242 O07242 GO:0004553,GO:0005975 3.2.1.- O07242 ML2516,ML2516c MMAR_0567 MSMEG_0645 Mb0323 NC_000962.3 Mycobrowser_v4 CDS 589183 590058 . + 0 Rv0499 Rv0499 Function unknown Conserved hypothetical protein Rv0499, (MTCY20G9.25), len: 291 aa. Conserved hypothetical protein, showing some similarity to AL031184|SC2A11_16|T34762 hypothetical protein from Streptomyces coelicolor (340 aa), FASTA scores: opt: 240, E(): 1.8e-07, (28.9% identity in 270 aa overlap). P9WKT9 No conserved hypotheticals P9WKT9 GO:0004143,GO:0007205 P9WKT9 ML2431,ML2431c MMAR_0825 MSMEG_0942 Mb0510 NC_000962.3 Mycobrowser_v4 CDS 386305 387099 . - 0 Rv0318c Rv0318c Unknown Probable conserved integral membrane protein Rv0318c, (MTCY63.23c), len: 264 aa. Probable conserved integral membrane protein, with some similarity to C-terminus of GUFA_MYXXA|Q06916 (254 aa), FASTA scores: opt: 157, E (): 0.0032, (28.3% identity in 198 aa overlap). Also similar to O26573 conserved protein from Methanobacterium thermoauto (259 aa), FASTA scores: opt: 173, E(): 5.2e-05, (32.7% identity in 214 aa overlap). Q6MX47 No cell wall and cell processes Q6MX47 Q6MX47 GO:0046873,GO:0030001,GO:0055085,GO:0016020 Q6MX47 MMAR_0595 Mb0326c NC_000962.3 Mycobrowser_v4 CDS 387148 387816 . + 0 Rv0319 pcp Removes 5-oxoproline from various penultimate amino acid residues except L-proline [catalytic activity: 5-oxoprolyl-peptide + H2O = 5-oxoproline + peptide]. Probable pyrrolidone-carboxylate peptidase Pcp (5-oxoprolyl-peptidase) (pyroglutamyl-peptidase I) (PGP-I) (pyrase) Rv0319, (MTCY63.24), len: 222 aa. Probable pcp, pyrrolidone-carboxylate peptidase, highly similar to others e.g. PCP_PSEFL|P42673 pyrrolidone-carboxylate peptidase from Pseudomonas fluorescens (213 aa), FASTA scores: opt: 478, E(): 7.5e-25, (40.2% identity in 219 aa overlap). Belongs to peptidase family C15 (thiol protease). P9WIJ5 No intermediary metabolism and respiration P0A5R4 P9WIJ5 GO:0005737,GO:0006508,GO:0016920,GO:0008234 3.4.19.3 P9WIJ5 MMAR_0598 Mb0327 NC_000962.3 Mycobrowser_v4 CDS 388582 389154 . + 0 Rv0321 dcd Involved in interconversion of dCTP and dUTP [catalytic activity: dCTP + H2O = dUTP + NH3]. Probable deoxycytidine triphosphate deaminase Dcd (dCTP deaminase) Rv0321, (MTCY63.26), len: 190 aa. Probable dcd (alternate gene names: dus or paxA), deoxycytidine triphosphate deaminase, equivalent to CAC32024.1|AL583925 probable deoxycytidine triphosphate deaminase from Mycobacterium leprae (190 aa). Also highly similar to others e.g. Q9X8W0|DCD_STRCO|7480599|T36613|SCH35.46 deoxycytidine triphosphate deaminase from Streptomyces coelicolor (191 aa); DCD_ECOLI|P28248|DUS|PAXA|B2065 deoxycytidine triphosphate deaminase from Escherichia coli strain K12 (193 aa), FASTA scores: opt: 408, E(): 2.7e-21, (43.1% identity in 188 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the dCTP deaminase family. The transcription of this CDS seems to be activated specifically in host granulomas (see citation below). P9WP17 No intermediary metabolism and respiration 2QLP,2QXX O07247 P9WP17 GO:0006229,GO:0005515,GO:0009220,GO:0008829 3.5.4.13 P9WP17 ML2507,ML2507c MMAR_0600 MSMEG_0678 Mb0329 NC_000962.3 Mycobrowser_v4 CDS 389260 390591 . + 0 Rv0322 udgA Possibly involved in polysaccharide biosynthesis [catalytic activity: UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH]. Probable UDP-glucose 6-dehydrogenase UdgA (UDP-GLC dehydrogenase) (UDP-GLCDH) (UDPGDH) Rv0322, (MTCY63.27), len: 443 aa. Probable udg (alternate gene name: rkpK), UDP-glucose 6-dehydrogenase , highly similar to others e.g. CAC44517.1|AL596138 putative UDP-glucose 6-dehydrogenase from Streptomyces coelicolor (447 aa); Q56812 UDP-glucose dehydrogenase from Xanthomonas campestris (445 aa), FASTA scores: opt: 713, E(): 0, (41.9% identity in 351 aa overlap); etc. Also similar to several GDP-mannose 6-dehydrogenase. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the UDP-glucose/GDP-mannose dehydrogenases family. O07248 No intermediary metabolism and respiration O07248 O07248 GO:0003979,GO:0055114,GO:0051287 1.1.1.22 O07248 ML2506,ML2506c MMAR_0603 MSMEG_0680 Mb0330 NC_000962.3 Mycobrowser_v4 CDS 390580 391251 . - 0 Rv0323c Rv0323c Function unknown Conserved hypothetical protein Rv0323c, (MTCY63.28c), len: 223 aa. Conserved hypothetical protein, similar to others e.g. YPJG_BACSU|P42981 hypothetical 24.8 kDa protein from Bacillus subtilis (224 aa), FASTA scores: opt: 182, E(): 1.3e-05, (27.5% identity in 211 aa overlap). Also some similarity to MLU15183_8 from Mycobacterium tuberculosis FASTA score: (32.0% identity in 147 aa overlap). Alternative nucleotide at position 390828 (T->C; S142G) has been observed. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L0T643 No conserved hypotheticals O07249 L0T643 L0T643 Mb0331c NC_000962.3 Mycobrowser_v4 CDS 421709 422416 . + 0 Rv0351 grpE Stimulates, jointly with DNAJ|Rv0352, the ATPase activity of DNAK|Rv0350. HELPS to release ADP from DNAK thus allowing DNAK to recycle more efficiently. Seems to be regulated negatively by HSPR (Rv0353 product). Probable GrpE protein (HSP-70 cofactor) Rv0351, (MTCY13E10.11), len: 235 aa. Probable grpE protein (HSP-70 cofactor), equivalent to CAC32012.1|AL583925 Hsp70 cofactor from Mycobacterium leprae (229 aa). Also highly similar to others eg Q05562|GRPE_STRCO|2127521|PN0643 GRPE protein from Streptomyces coelicolor (225 aa). Contains grpE protein signature (PS01071). Belongs to the GrpE family. P9WMT5 No virulence, detoxification, adaptation P32724 P9WMT5 GO:0051087,GO:0005739,GO:0006457,GO:0042803,GO:0030150,GO:0006950,GO:0000774 P9WMT5 ML2495,ML2495c MMAR_0638 MSMEG_0710 Mb0359 NC_000962.3 Mycobrowser_v4 CDS 392696 394045 . - 0 Rv0327c cyp135A1 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Possible cytochrome P450 135A1 Cyp135A1 Rv0327c, (MT0342, MTCY63.32c), len: 449 aa. Possible cyp135A1, cytochrome P450, similar to cytochrome P-450 monoxygenases and other cytochrome P-450 related enzymes e.g. FQ12609 putative P450 monooxygenase (506 aa), FASTA scores: opt: 276, E() : 1.7e-11, (27.9% identity in 433 aa overlap). Also similar to other Mycobacterium tuberculosis proteins e.g. MTV039.06|Rv0568 putative cytochrome P450 (472 aa); MTCI5.10 cytochrome p450 FASTA score: (30.4% identity in 434 aa overlap). Contains cytochrome P450 cysteine heme-iron ligand signature (PS00086). Belongs to the cytochrome P450 family. Alternative start possible at 33706 but no RBS. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WPN1 No intermediary metabolism and respiration O08447 P9WPN1 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPN1 Mb0334c NC_000962.3 Mycobrowser_v4 CDS 394111 394713 . + 0 Rv0328 Rv0328 Possibly involved in a transcriptional mechanism. Possible transcriptional regulatory protein (possibly TetR/AcrR-family) Rv0328, (MTCY63.33), len: 200 aa. Possible transcription regulator, TetR/acrR family, similar in part to various hypothetical transcriptional regulators e.g. T36696|4726006|CAB41735.1|AL049731 probable regulatory protein from Streptomyces coelicolor (197 aa). Also some similarity with YX44_MYCTU|Q10829 hypothetical transcriptional regulator from Mycobacterium tuberculosis (195 aa), FASTA scores: opt: 154, E(): 0.00061, (26.7% identity in 202 aa overlap). Contains probable helix-turn helix motif from aa 27-48 (Score 1408, +3.98 SD). Seems to belong to the TetR/AcrR family of transcriptional regulators. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07252 No regulatory proteins O07252 O07252 GO:0006350,GO:0003700,GO:0006355 O07252 Mb0335 NC_000962.3 Mycobrowser_v4 CDS 394694 395320 . - 0 Rv0329c Rv0329c Function unknown Conserved hypothetical protein Rv0329c, (MTCY63.34c), len: 208 aa. Conserved hypothetical protein, showing some similarity with others hypothetical proteins and methyltransferases e.g. MitM|AF127374_14 methyltransferase from Streptomyces lavendulae (283 aa), FASTA scores: opt: 242, E(): 1.8e-08, (37.2% identity in 145 aa overlap); Q48938 from Methanosarcina barkeri (262 aa), FASTA scores: opt: 194, E(): 3.6e-06, (31.1% identity in 119 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07253 No conserved hypotheticals O07253 O07253 GO:0008168,GO:0008152 O07253 MSMEG_2632 Mb0336c NC_000962.3 Mycobrowser_v4 CDS 395347 396087 . - 0 Rv0330c Rv0330c Unknown Hypothetical protein Rv0330c, (MTCY63.35c), len: 246 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07254 No conserved hypotheticals O07254 O07254 GO:0006350,GO:0003700,GO:0006355 Mb0337c NC_000962.3 Mycobrowser_v4 CDS 1240187 1240885 . + 0 Rv1115 Rv1115 Unknown Possible exported protein Rv1115, (MTCY22G8.04), len: 232 aa. Possible exported protein, contains possible N-terminal signal sequence. O06567 No cell wall and cell processes O06567 O06567 ML0940 MMAR_4345 Mb1145 NC_000962.3 Mycobrowser_v4 CDS 4387365 4387895 . - 0 Rv3902c Rv3902c Unknown Hypothetical protein Rv3902c, (MTCY15F10.10), len: 176 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O05443 No conserved hypotheticals O05443 O05443 Mb3932c NC_000962.3 Mycobrowser_v4 CDS 399535 400050 . - 0 Rv0335c PE6 Function unknown PE family protein PE6 Rv0335c, (MTCY279.02c), len: 171 aa. PE6, Member of the Mycobacterium tuberculosis PE family (see Brennan & Delogu 2002); contains short region of similarity to part of the unique N-terminus of the Mycobacterium tuberculosis PGRS family of Glycine-rich proteins e.g. Y03A_MYCTU|Q10637 hypothetical glycine-rich 49.6 kd protein (603 aa), FASTA scores: opt: 219, E(): 1.1e-08, (51.5% identity in 66 aa overlap). L7N648 No PE/PPE O86338 L7N648 L7N648 Mb0342c NC_000962.3 Mycobrowser_v4 CDS 400192 401703 . + 0 Rv0336 Rv0336 Function unknown Conserved 13E12 repeat family protein Rv0336, (MTCY279.03), len: 503 aa. Part of Mycobacterium tuberculosis 13E12 repeat family; almost identical to Rv0515|MTCY20G10.05 hypothetical protein from Mycobacterium tuberculosis FASTA scores: (99.8% identity in 503 aa overlap), possibly due to a recent gene duplication. Also similar to other Mycobacterium tuberculosis hypothetical proteins e.g. Rv1148c, Rv1945, etc. O33266 No insertion seqs and phages O33266 O33266 GO:0003676,GO:0004519 MMAR_5187 MSMEG_5026 Mb0343 NC_000962.3 Mycobrowser_v4 CDS 401873 403162 . - 0 Rv0337c aspC Generates oxaloacetate and L-glutamate from L-aspartate and 2-oxoglutarate [catalytic activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate]. Probable aspartate aminotransferase AspC (transaminase A) (ASPAT) Rv0337c, (MTCY279.04c), len: 429 aa. Probable aspC, aspartate aminotransferase (transaminase A), equivalent to CAC32019.1|AL583925 probable aspartate aminotransferase from Mycobacterium leprae (437 aa). Also highly similar to many e.g. Q48143|U32823 aspartate aminotransferase (404 aa), FASTA scores: opt: 1646, E(): 0, (57.2% identity in 404 aa overlap). Also some similarity to Rv3565|MTCY06G11.12 from Mycobacterium tuberculosis FASTA score: (27.2% identity in 383 aa overlap). Belongs to class-I of pyridoxal-phosphate-dependent aminotransferases. Cofactor: pyridoxal phosphate. P9WQ91 No intermediary metabolism and respiration P63498 P9WQ91 GO:0004069,GO:0009058,GO:0005737,GO:0030170,GO:0016847 2.6.1.1 P9WQ91 ML2502 MMAR_0610 MSMEG_0688 Mb0344c NC_000962.3 Mycobrowser_v4 CDS 443067 444197 . - 0 Rv0365c Rv0365c Function unknown: may be involved in the ability to survive in macrophages. Conserved protein Rv0365c, (MTCY13E10.27c), len: 376 aa (start uncertain). Conserved protein (see citation below), very similar to G388212|CAA35191.1, a truncated ORF immediately upstream of the Corynebacterium glutamicum fda gene encoding fructose-1,6-biphosphate aldolase (304 aa), FASTA scores: E(): 7.1e-19, (42.2% identity in 296 aa overlap). O06315 No conserved hypotheticals O06315 O06315 GO:0003824 O06315 MMAR_0667 MSMEG_0740 Mb0372c NC_000962.3 Mycobrowser_v4 CDS 2139076 2139687 . - 0 Rv1890c Rv1890c Unknown Hypothetical protein Rv1890c, (MTCY180.28), len: 203 aa. Hypothetical unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). O07742 No conserved hypotheticals O07742 O07742 O07742 ML2026 MMAR_2781 MSMEG_0101 Mb1923c NC_000962.3 Mycobrowser_v4 CDS 408634 409173 . + 0 Rv0340 Rv0340 Function unknown Conserved protein Rv0340, (MTCY279.07), len: 179 aa. Conserved protein; MEME-mast analysis shows similarity to product of downstream gene, Rv0341|iniB. O33270 No conserved hypotheticals O33270 EMB,INH MMAR_0613 Mb0347 NC_000962.3 Mycobrowser_v4 CDS 410838 412760 . + 0 Rv0342 iniA Unknown Isoniazid inductible gene protein IniA Rv0342, iniA, (MTCY13E10.02), len: 640 aa. IniA, isoniazid-inducible gene, (see citations below). Shows slight similarity to some hypothetical bacterial proteins e.g. P40983|YOR6_THER hypothetical protein (402 aa), FASTA scores: opt: 242, E(): 1.4e-07, (22.3% identity in 349 aa overlap). Also some similarity to downstream ORF Rv0343|iniC. Possible transmembrane stretch around residue 490. Alternative start site exists at 410824. Contains a phosphopantetheine attachment site motif suggestive of an acyl carrier protein. Note that the iniA gene is also induced by the antibiotic ethambutol, an agent that inhibits cell wall biosynthesis by a mechanism that is distinct from isoniazid. P9WJ99 No cell wall and cell processes O06293 P9WJ99 GO:0003924,GO:0016021,GO:0005886,GO:0046677,GO:0005525 EMB,INH P9WJ99 MMAR_0615 MSMEG_0695 Mb0349 NC_000962.3 Mycobrowser_v4 CDS 436860 437507 . + 0 Rv0358 Rv0358 Function unknown Conserved protein Rv0358, (MTCY13E10.20), len: 215 aa. Conserved protein, highly similar to ML0281|AL023514|MLCB4_14 conserved hypothetical protein from Mycobacterium leprae (229 aa), FASTA scores: opt: 852, E(): 0, (62.9% identity in 229 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06308 No conserved hypotheticals O06308 ML0281 MMAR_0679 MSMEG_0758 Mb0365 NC_000962.3 Mycobrowser_v4 CDS 444222 444815 . - 0 Rv0366c Rv0366c Function unknown Conserved hypothetical protein Rv0366c, (MTV036.01c), len: 197 aa. Conserved hypothetical protein, showing weak similarity to HI1395|P44173|YD95_HAEIN hypothetical protein from Haemophilus influenzae (140 aa), FASTA scores: opt: 152, E(): 0.0015, (27.0% identity in 126 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS00850 Glycine radical signature. O53701 No conserved hypotheticals O53701 O53701 GO:0001906,GO:0005524 O53701 MSMEG_3436 Mb0373c NC_000962.3 Mycobrowser_v4 CDS 456915 457823 . - 0 Rv0381c Rv0381c Unknown Hypothetical protein Rv0381c, (MTV036.16c), len: 302 aa. Hypothetical unknown protein. Equivalent to AAK44616.1 from Mycobacterium tuberculosis strain CDC1551 (254 aa) but longer 48 aa. O53716 No conserved hypotheticals O53716 ML2486,ML2486c MMAR_0650 MSMEG_0738 Mb0388c NC_000962.3 Mycobrowser_v4 CDS 1184015 1184878 . + 0 Rv1061 Rv1061 Function unknown Conserved protein Rv1061, (MTV017.14), len: 287 aa. Conserved protein, similar to hypothetical proteins from various bacteria e.g. D64002|SYCSLRD_75 Synechocystis sp. PCC6803 (304 aa),FASTA scores: opt: 245, E():1.2e-09, (27.1% identity in 258 aa overlap). O53409 No conserved hypotheticals O53409 O53409 GO:0006541,GO:0009113,GO:0004044 MMAR_4405 Mb1090 NC_000962.3 Mycobrowser_v4 CDS 3131770 3132714 . - 0 Rv2824c Rv2824c Unknown Hypothetical protein Rv2824c, (MTCY16B7.18), len: 314 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WPJ1 No conserved hypotheticals P71628 P9WPJ1 P9WPJ1 Mb2848c NC_000962.3 Mycobrowser_v4 CDS 414381 414941 . - 0 Rv0344c lpqJ Unknown Probable lipoprotein LpqJ Rv0344c, (MTCY13E10.04c), len: 186 aa. Probable lipoprotein, without homology. Has an appropriately positioned prokaryotic lipoprotein signature (PS00013). O06295 No cell wall and cell processes O06295 MMAR_0620 MSMEG_0704 Mb0351c NC_000962.3 Mycobrowser_v4 CDS 415050 415460 . + 0 Rv0345 Rv0345 Function unknown Conserved hypothetical protein Rv0345, (MTCY13E10.05), len: 136 aa. Conserved hypothetical protein, similar to other hypothetical proteins e.g. AL13282 4|SCAH10_9 hypothetical protein from Streptomyces coelicolor (207 aa), FASTA scores: opt: 188, E(): 1.5e-05, (41.0% identity in 117 aa overlap). O06296 No conserved hypotheticals O06296 O06296 MMAR_0624 MSMEG_1806 Mb0352,Mb0353 NC_000962.3 Mycobrowser_v4 CDS 418293 418946 . + 0 Rv0348 Rv0348 Could be involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0348, (MTCY13E10.08), len: 217 aa. Possible transcriptional regulator, showing some similarity to O53334|RV3188|MTV014.32 conserved hypothetical protein from Mycobacterium tuberculosis (115 aa), FASTA score: (30.0% identity in 100 aa overlap). Contains probable helix-turn helix motif from aa 89-110 (Score 1407, +3.98 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06299 No regulatory proteins O06299 O06299 Mb0356 NC_000962.3 Mycobrowser_v4 CDS 419835 421712 . + 0 Rv0350 dnaK Acts as a chaperone. Involved in induction by stress conditions e.g. heat shock. Possibly has an ATPase activity. Seems to be regulated positively by sigh (Rv3223c product) and negatively by HSPR (Rv0353 product). Probable chaperone protein DnaK (heat shock protein 70) (heat shock 70 kDa protein) (HSP70) Rv0350, (MTCY13E10.10), len: 625 aa. Probable dnaK (alternate gene name: hsp70), 70 kDa heat shock protein (see citations below), equivalent to AAA25362.1|M95576|1924344A|738248 heat shock protein 70 from Mycobacterium leprae (621 aa); and DNAK_MYCPA|Q00488 (623 aa), FASTA scores: opt: 3678, E(): 0, (92.3% identity in 625 aa overlap). Also highly similar to others e.g. Q05558|DNAK_STRCO|453231|CAA54606.1|X77458 chaperone protein DNAK from Streptomyces coelicolor (618 aa). Has probably an ATPase activity. Note that this sequence differs from DNAK_MYCTU|P32723 (609 aa), due to a frameshift near the N-terminus. Belongs to the heat shock protein 70 family. P9WMJ9 No virulence, detoxification, adaptation P0A5B9 P9WMJ9 GO:0006457,GO:0006950,GO:0051082,GO:0005524 P9WMJ9 ML2496,ML2496c MMAR_0637 MSMEG_0709 Mb0358 NC_000962.3 Mycobrowser_v4 CDS 2307821 2308156 . + 0 Rv2050 Rv2050 Unknown Conserved protein Rv2050, (MTV018.37), len: 111 aa. Conserved protein, similar to many. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WHJ5 No conserved hypotheticals P9WHJ5 P9WHJ5 ML1439 MMAR_3027 MSMEG_3858 Mb2076 NC_000962.3 Mycobrowser_v4 CDS 423639 424019 . + 0 Rv0353 hspR Involved in transcriptional regulation (repression) of heat shock proteins e.g. DNAK|Rv0350, GRPE|Rv0351, DNAJ1|Rv0352. Binds to three inverted repeats (IR1-IR3) in the promoter region of the DNAK operon. Induction: by heat shock. Probable heat shock protein transcriptional repressor HspR (MerR family) Rv0353, (MTCY13E10.13), len: 126 aa. Probable hspR, heat shock regulatory protein (see Stewart et al., 2001), merR family, highly similar to others e.g. HspR|P40183 heat shock regulatory protein from Streptomyces coelicolor (151 aa), FASTA scores: E(): 4.9e-22, (55.7% identity in 140 aa overlap), that binds to three inverted repeats (IR1-IR3) in the promoter region of the dnaK operon. Has possible coiled coil region in C-terminal half. Belongs to the MerR family of transcriptional regulators. O06302 No virulence, detoxification, adaptation O06302 O06302 GO:0006355,GO:0006950,GO:0003700,GO:0000166 O06302 ML2493,ML2493c MMAR_0640 MSMEG_0713 Mb0361 NC_000962.3 Mycobrowser_v4 CDS 424777 434679 . - 0 Rv0355c PPE8 Function unknown PPE family protein PPE8 Rv0355c, (MTCY13E10.15c, MTCY13E10.16c, MTCY13E10.17c), len: 3300 aa. PPE8, Member of the Mycobacterium tuberculosis PPE family, similar to others e.g. AL009198|MTV004_5 from Mycobacterium tuberculosis (3716 aa), FASTA scores: opt: 2906, E(): 0, (40.9% identity in 3833 aa overlap); MTV004_3 FASTA scores: (39.0% identity in 3531 aa overlap); etc. Gene contains large number of clustered Major Polymorphic Tandem Repeats (MPTR). Related to MTCY13E10.16c, E(): 0; MTCY13E10.17c, E(): 0; MTCY48.17, E(): 0; MTCY98.0034c, E(): 0; MTCY03C7.23 E(): 0; MTCY98.0031c, E(): 0; MTCY31.06c, E(): 5.6e-17; MTCY359.33, E(): 2.3e-16. Nucleotide position 426909 in the genome sequence has been corrected, A:C resulting in W2591G. I6Y7L4 No PE/PPE Q6MX44 I6Y7L4 MMAR_0642 Mb0362c NC_000962.3 Mycobrowser_v4 CDS 434830 435474 . - 0 Rv0356c Rv0356c Function unknown Conserved protein Rv0356c, (MTCY13E10.18c), len: 214 aa. Conserved protein, equivalent to AL023514|MLCB4_12 conserved hypothetical protein from Mycobacterium leprae (218 aa), FASTA scores: opt: 1067, E(): 0, (73.4% identity in 214 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06307 No conserved hypotheticals O06307 O06307 O06307 ML0279,ML0279c MMAR_0681 MSMEG_0760 Mb0363c NC_000962.3 Mycobrowser_v4 CDS 437518 438297 . + 0 Rv0359 Rv0359 Unknown Probable conserved integral membrane protein Rv0359, (MTCY13E10.21), len: 259 aa. Probable conserved integral membrane protein, highly similar to hypothetical or other membrane proteins e.g. AL133220|SCC75A_6|T50569 probable membrane protein from Streptomyces coelicolor (265 aa), FASTA scores: opt: 642, E(): 0, (43.1% identity in 248 aa overlap); P70995 hypothetical 24.7 kDa protein from Bacillus subtilis (219 aa), FASTA scores: E(): 1.5e-12, (31.3% identity in 192 aa overlap). Contains neutral zinc metallopeptidases, zinc-binding region signature (PS00142). L0T550 No cell wall and cell processes O06309 L0T550 GO:0006508,GO:0004222 ML0282 MMAR_0678 MSMEG_0756 Mb0366 NC_000962.3 Mycobrowser_v4 CDS 438822 439649 . + 0 Rv0361 Rv0361 Unknown Probable conserved membrane protein Rv0361, (MTCY13E10.23), len: 275 aa. Probable conserved membrane protein (has hydrophobic stretch from residues 132-156), equivalent to AL023514|MLCB4_17|AA18949.1|AL023514 putative membrane protein from Mycobacterium leprae (292 aa), FASTA scores: opt: 1044, E(): 0, (58.6% identity in 292 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06311 No cell wall and cell processes O06311 O06311 ML0285 MMAR_0671 MSMEG_0753 Mb0368 NC_000962.3 Mycobrowser_v4 CDS 474122 474841 . - 0 Rv0394c Rv0394c Unknown Possible secreted protein Rv0394c, (MTCY04D9.06c), len: 239 aa. Possible secreted protein, sharing no homology with other proteins. Has hydrophobic stretch at its N-terminus. P95202 No cell wall and cell processes P95202 P95202 Mb0400c NC_000962.3 Mycobrowser_v4 CDS 441265 442299 . - 0 Rv0363c fba Involved in glycolysis (at the sixth step) [catalytic activity: D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate]. Probable fructose-bisphosphate aldolase Fba Rv0363c, (MTCY13E10.25c), len: 344 aa. Probable fba (alternate gene name: fda), fructose bisphosphate aldolase , equivalent to AL023514|MLCB4_18|O69600|ALF_MYCLE fructose-bisphosphate aldolase from Mycobacterium leprae (345 aa), FASTA scores: opt: 1995, E(): 0, (87.7% identity in 342 aa overlap). Also highly similar to others. Belongs to class II fructose-bisphosphate aldolase family. Cofactor: zinc. P9WQA3 No intermediary metabolism and respiration 3EKL,3EKZ,3ELF,4A21,4A22 P67475 P9WQA3 GO:0006096,GO:0008270,GO:0004332 4.1.2.13 P9WQA3 ML0286,ML0286c MMAR_0669 MSMEG_0752 Mb0370c NC_000962.3 Mycobrowser_v4 CDS 446531 447046 . - 0 Rv0369c Rv0369c Function unknown; probably involved in cellular metabolism. Possible membrane oxidoreductase Rv0369c, (MTV036.04c), len: 171 aa. Possible membrane protein oxidoreductase, similar to ORF 4 of the Pseudomonas thermocarboxydovorans protein of cutA-cutB-cutC gene cluster: X77931|PTC2CUTAC_4 ORF4 from Pseudomonas thermocarboxydovorans (171 aa), FASTA scores: opt: 226, E(): 9.8e-08, (31.3% identity in 166 aa overlap). Also similar to MTV036.05, MTV036.08, MTV036.09, and MTV026.10. O53704 No intermediary metabolism and respiration O53704 O53704 GO:0016491,GO:0055114 1.-.-.- O53704 ML2480,ML2480c Mb0376c NC_000962.3 Mycobrowser_v4 CDS 447147 448043 . - 0 Rv0370c Rv0370c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0370c, (MTV036.05c), len: 298 aa. Possible oxidoreductase, similar to many hypothetical proteins, but also similar to ORF4|X82447|OCCOXMSL4_4 Protein of coxMSL gene cluster from Pseudomonas/Oligotropha carboxidovorans (295 aa), FASTA scores: opt: 851, E(): 0, (48.2% identity in 282 aa overlap); AJ224684|BJAJ4684_3 cooxS from Bradyrhizobium japonicum (302 aa), FASTA scores: opt: 881, E(): 0, (47.6% identity in 290 aa overlap). Also highly similar to MTCY428_21 from Mycobacterium tuberculosis. Contains PS00017 ATP/GTP-binding site motif A (P-loop). O53705 No intermediary metabolism and respiration O53705 O53705 GO:0016887,GO:0055114,GO:0016491,GO:0005524 1.-.-.- ML2481,ML2481c MMAR_0660 MSMEG_0748 Mb0377c NC_000962.3 Mycobrowser_v4 CDS 532396 532911 . + 0 Rv0443 Rv0443 Function unknown Conserved protein Rv0443, (MTV037.07), len: 171 aa. Conserved protein, highly similar to AL049863|SC5H1_23|T35339 hypothetical protein from Streptomyces coelicolor (171 aa), FASTA scores: opt: 561, E(): 2.3e-32, (49.7% identity in 165 aa overlap); and CAC42482.1|AJ318385 hypothetical protein from Amycolatopsis mediterranei (163 aa). O53728 No conserved hypotheticals O53728 O53728 O53728 Mb0451 NC_000962.3 Mycobrowser_v4 CDS 1589386 1589901 . + 0 Rv1413 Rv1413 Function unknown Conserved hypothetical protein Rv1413, (MTCY21B4.30), len: 171 aa. Conserved hypothetical protein, similar to part of AB010956|AB010956_1 metal-activated pyridoxal enzyme from Arthrobacter sp. (379 aa), FASTA scores: opt: 187, E(): 0.00026, (29.0% identity in 162 aa overlap). P9WLY5 No conserved hypotheticals P64843 P9WLY5 P9WLY5 Mb1448 NC_000962.3 Mycobrowser_v4 CDS 451800 452279 . - 0 Rv0374c Rv0374c Function unknown; probably involved in cellular metabolism [catalytic activity: CO + H(2)O + acceptor = CO(2) + reduced acceptor]. Probable carbon monoxyde dehydrogenase (small chain) Rv0374c, (MTV036.09c), len: 159 aa. Probable carbon monoxide dehydrogenase, small chain, highly similar to others e.g. B56279|5822285|X82447|OCCOXMSL4_2|COXS carbon-monoxide dehydrogenase small chain from Pseudomonas/Oligotropha carboxydovorans (166 aa), FASTA scores: opt: 662, E(): 0, (59.3% identity in 150 aa overlap); CAA12063.1|AJ224684 putative carbon monoxide dehydrogenase small subunit from Bradyrhizobium japonicum (161 aa); S49123|509390|CAA54901.1|X77931|CUTC carbon-monoxide dehydrogenase small chain from Pseudomonas thermocarboxydovorans (163 aa); etc. O53709 No intermediary metabolism and respiration O53709 O53709 GO:0009055,GO:0051536,GO:0046872,GO:0055114,GO:0018492 1.2.99.2 O53709 ML2499 MMAR_0656 MSMEG_0745 Mb0381c NC_000962.3 Mycobrowser_v4 CDS 452294 453154 . - 0 Rv0375c Rv0375c Function unknown; probably involved in cellular metabolism [catalytic activity: CO + H(2)O + acceptor = CO(2) + reduced acceptor]. Probable carbon monoxyde dehydrogenase (medium chain) Rv0375c, (MTV036.10c), len: 286 aa. Probable carbon monoxide dehydrogenase, medium chain, similar to others e.g. AAD00361.1|U80806|CUTM carbon monoxide dehydrogenase middle subunit from Hydrogenophaga pseudoflava (287 aa); S49122|509389|CAA54900.1|X77931|CUTB carbon-monoxide dehydrogenase medium chain from Pseudomonas thermocarboxydovorans (287 aa); A56279|809564|CAA57827.1|X82447|OCCOXMSL4_1|COXM|CODH carbon-monoxide dehydrogenase medium chain from Pseudomonas/Oligotropha carboxydovorans (288 aa), FASTA scores: opt: 594, E(): 0, (37.5% identity in 277 aa overlap); etc. I6Y7N2 No intermediary metabolism and respiration O53710 I6Y7N2 GO:0018492,GO:0055114,GO:0050660 1.2.99.2 I6Y7N2 MMAR_0655 MSMEG_0744 Mb0382c NC_000962.3 Mycobrowser_v4 CDS 466672 467406 . - 0 Rv0387c Rv0387c Function unknown Conserved hypothetical protein Rv0387c, (MTV036.22c), len: 244 aa. Conserved hypothetical protein, showing some similarity to MTCI237.20c, and M17282|HUMEL20_1 Human elastin gene, exon 1, Elastin (687 aa), FASTA scores: opt: 193, E(): 0.35, (34.4% identity in 189 aa overlap). L0T6I4 No conserved hypotheticals O53721 L0T6I4 ML0278 Mb0394c NC_000962.3 Mycobrowser_v4 CDS 476679 477320 . - 0 Rv0398c Rv0398c Unknown Possible secreted protein Rv0398c, (MTCY04D9.10c), len: 213 aa. Possible secreted protein, sharing no homology with other proteins. Has potential signal sequence with hydrophobic stretch from aa 7-25. P95206 No cell wall and cell processes P95206 ML0274 MMAR_0695 Mb0404c NC_000962.3 Mycobrowser_v4 CDS 498300 499619 . - 0 Rv0412c Rv0412c Unknown Possible conserved membrane protein Rv0412c, (MTCY22G10.08c), len: 439 aa. Possible conserved membrane protein, equivalent to AL035159|MLCB1450_16|T44737 probable membrane protein from Mycobacterium leprae (403 aa), FASTA scores: opt: 2027, E(): 0, (80.4% identity in 403 aa overlap). Also some similarity with CAB71201.1|AL138538 putative secreted protein from Streptomyces coelicolor (429 aa). P96258 No cell wall and cell processes P96258 P96258 ML0302 MMAR_0715 MSMEG_0788 Mb0420c NC_000962.3 Mycobrowser_v4 CDS 2472493 2473242 . + 0 Rv2208 cobS Involved in cobalamin biosynthesis Probable cobalamin 5'-phosphate synthase CobS Rv2208, (MTCY190.19), len: 249 aa. Probable cobS, cobalamin 5'-phosphate synthase; similarity to SW:COBS_ECOLI P36561 cobalamin (5'-phosphate) synthase (28.0% identity in 243 aa overlap) P9WP91 No intermediary metabolism and respiration Q10397 P9WP91 GO:0009236,GO:0015420,GO:0016021,GO:0005886,GO:0008818 2.-.-.- ML0867,ML0867c MMAR_3253 MSMEG_4277 Mb2231 NC_000962.3 Mycobrowser_v4 CDS 455637 455858 . + 0 Rv0378 Rv0378 Function unknown Conserved hypothetical glycine rich protein Rv0378, (MTV036.13), len: 73 aa. Conserved hypothetical gly-rich protein, showing some similarity to Mycobacterium tuberculosis PE_PGRS family; also similar to MTCY06H11_16|Z85982 hypothetical glycine-rich 88.5 KD protein (1011 aa), FASTA scores: opt: 237, E(): 0.0032, (58.7% identity in 63 aa overlap); MTV043_25. O53713 No conserved hypotheticals O53713 MMAR_2795 Mb0385 NC_000962.3 Mycobrowser_v4 CDS 455977 456192 . + 0 Rv0379 secE2 Thought to be involved in protein transport (export). Possible protein transport protein SecE2 Rv0379, (MTV036.14), len: 71 aa. Possible secE2, protein transport protein, showing similarity with P27340|S61G_SULSO|SECE preprotein translocase SECE subunit (protein transport protein SEC61 gamma subunit homolog) from Sulfolobus acidocaldarius (65 aa), FASTA scores: opt: 79, E(): 4.7. (30.3% identity in 66 aa overlap); and hypothetical proteins e.g. Q9HPW4|VNG1446H hypothetical protein from Halobacterium sp. strain NRC-1 (77 aa); Q9I794|PA0038 hypothetical protein from Pseudomonas aeruginosa (71 aa); etc. Also highly similar to U85467|MTU85467_1 hypothetical Mycobacterium tuberculosis protein from a patient isolate (116 aa), FASTA scores: opt: 443, E(): 7.7e-29, (98.6% identity in 71 aa overlap). Note that for Rv0379|MTV036.14, a translation initiation region different to the one in U85467|MTU85467_1 was chosen. Could be a part of the prokaryotic protein translocation apparatus which comprise SECA|Rv3240c, SECD|Rv2587c, SECE|Rv0638, SECF|Rv2586c, SECG|Rv1440 and SECY|Rv0732. Q6MX43 No cell wall and cell processes 3ONR Q6MX43 Q6MX43 Q6MX43 MMAR_0652 Mb0386 NC_000962.3 Mycobrowser_v4 CDS 456268 456819 . - 0 Rv0380c Rv0380c Possibly causes methylation of RNA. Possible RNA methyltransferase (RNA methylase) Rv0380c, (MTV036.15c), len: 183 aa. Possible RNA methyltransferase, equivalent to CAC32002.1|AL583925 possible RNA methyltransferase from Mycobacterium leprae (182 aa). Also some similarity with others methyltransferases e.g. P19396|TRMH_ECOLI|78514|JV0043 tRNA (guanosine-2'-O-)-methyltransferase (tRNA methyltransferase) from Escherichia coli (229 aa), FASTA scores: opt: 227, E(): 1.4e-09, (28.9% identity in 166 aa overlap). Also similar to Rv0881, Rv3579c, Rv1644 from Mycobacterium tuberculosis. O53715 No intermediary metabolism and respiration O53715 O53715 GO:0008173,GO:0006396,GO:0003723 2.1.1.- O53715 ML2485,ML2485c MMAR_0651 MSMEG_0739 Mb0387c NC_000962.3 Mycobrowser_v4 CDS 467459 468001 . - 0 Rv0388c PPE9 Function unknown PPE family protein PPE9 Rv0388c, (MTV036.23c), len: 180 aa. PPE9, Member of the Mycobacterium tuberculosis PPE family, highly similar to others e.g. MTCY10G2_10|Z92539 from Mycobacterium tuberculosis (391 aa), FASTA scores: opt: 667, E(): 0, (58.3% identity in 180 aa overlap) but much shorter. L0T6B3 No PE/PPE Q6MX42 L0T6B3 L0T6B3 ML0277 NC_000962.3 Mycobrowser_v4 CDS 566776 567222 . + 0 Rv0477 Rv0477 Unknown Possible conserved secreted protein Rv0477, (MTCY20G9.03), len: 148 aa. Possible conserved secreted protein, equivalent to CAC31969.1|AL583925 hypothetical protein from Mycobacterium leprae (123 aa). Also similar to G83406|PA1914 conserved hypothetical protein from Pseudomonas aeruginosa (408 aa). Contains possible N-terminal signal sequence. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WKV9 No cell wall and cell processes P64697 P9WKV9 ML2452,ML2452c MMAR_0802 MSMEG_0921 Mb0487 NC_000962.3 Mycobrowser_v4 CDS 2447605 2447994 . - 0 Rv2186c Rv2186c Unknown Conserved hypothetical protein Rv2186c, (MTV021.19c), len: 129 aa. Conserved hypothetical protein, equivalent to hypothetical Mycobacterium leprae protein ML0888 (135 aa). FASTA scores: ML0888 opt: 704, E(): 2.9e-43; 80.000% identity in 130 aa overlap CAA18694.1| (AL022602). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53520 No conserved hypotheticals O53520 ML0888 MMAR_3230 MSMEG_4252 Mb2208c NC_000962.3 Mycobrowser_v4 CDS 458461 459315 . - 0 Rv0383c Rv0383c Unknown Possible conserved secreted protein Rv0383c, (MTV036.18c), len: 284 aa. Possible conserved secreted protein, with hydrophobic stretch in N-terminus and Pro-rich C-terminus. Equivalent to CAC32006.1|AL583925 possible secreted protein from Mycobacterium leprae (286 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53718 No cell wall and cell processes O53718 ML2489,ML2489c MMAR_0647 MSMEG_0736 Mb0390c NC_000962.3 Mycobrowser_v4 CDS 462135 463307 . + 0 Rv0385 Rv0385 Function unknown; probably involved in cellular metabolism. Probable monooxygenase Rv0385, (MTV036.20), len: 390 aa. Probable monooxygenase, similar to T37003|5738846|CAB52917.1|AL109949 probable flavohemoprotein from Streptomyces coelicolor (435 aa); and similar in part (C-termini) to various monooxygenases e.g. P19734|DMPP_PSESP|94993|F37831 phenol hydroxylase P5 protein (phenol 2-monooxygenase P5 component) from Pseudomonas putida (353 aa), FASTA scores: opt: 363, E(): 4.2e-16, (31.8% identity in 255 aa overlap); S47292|2120861|pir|S70085 phenol 2-monooxygenase chain mopP from Acinetobacter calcoaceticus (350 aa); P21394|XYLA_PSEPU|94933|B37316 xylene monooxygenase electron transfer component [includes: ferredoxin; ferredoxin--NAD(+) reductase] from Pseudomonas putida plasmid pWW0 (350 aa); AAC38360.1|AF043544|NtnMA|ntnA reductase component of 4-nitrotoluene monooxygenase from Pseudomonas sp. (328 aa); etc. Q7ARS9 No intermediary metabolism and respiration Q7ARS9 Q7ARS9 GO:0004497,GO:0055114,GO:0019825,GO:0015671,GO:0005344,GO:0020037 1.-.-.- Q7ARS9 ML2492 MMAR_0644 MSMEG_0719 Mb0392 NC_000962.3 Mycobrowser_v4 CDS 463411 466668 . + 0 Rv0386 Rv0386 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably LuxR/UhpA-family) Rv0386, (MTV036.21), len: 1085 aa. Probable regulatory protein, LuxR/uhpA family, highly similar to CAC30706.1|AL583923 possible transcriptional regulator from Mycobacterium leprae (1106 aa). Also similar in part to other regulatory proteins e.g. CAB95788.1|AL359949 putative multi-domain regulatory protein from Streptomyces coelicolor (780 aa); N-terminus of CAB92369.1|AL356612 putative AfsR-like regulatory protein from Streptomyces coelicolor (1114 aa); N-terminus of NP_107139.1|14026327|BAB52925.1|AP003009 transcriptional regulator from Mesorhizobium loti (952 aa); AFSR_STRCO|P25941 regulatory protein afsr from Streptomyces coelicolor (993 aa), FASTA scores: opt: 224, E() : 1.1e-06, (26.1% identity in 867 aa overlap); etc. Also similar to many putative Mycobacterium tuberculosis regulatory proteins e.g. AL0212|MTV008_44 (1137 aa), FASTA scores: opt: 3756, E(): 0, (56.7% identity in 1089 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00622 Bacterial regulatory proteins, luxR family signature and probable helix-turn-helix motif at aa 1042-1063 (Score 1025, +2.68 S D). Belongs to the LuxR/UhpA family of transcriptional regulators. O53720 No regulatory proteins O53720 O53720 GO:0006915,GO:0009190,GO:0006952,GO:0005622,GO:0016849,GO:0006355,GO:0043565,GO:0003700,GO:0000156,GO:0000160,GO:0005524 O53720 Mb0393 NC_000962.3 Mycobrowser_v4 CDS 1241003 1241188 . + 0 Rv1116 Rv1116 Unknown Hypothetical protein Rv1116, (MTCY22G8.05), len: 61 aa. Hypothetical unknown protein. O06568 No conserved hypotheticals O06568 Mb1146 NC_000962.3 Mycobrowser_v4 CDS 469591 470013 . + 0 Rv0390 Rv0390 Function unknown Conserved protein Rv0390, (MTCY04D9.02), len: 140 aa. Conserved protein, equivalent to AL023514|MLCB4_11|CAA18942.1|AL023514 hypothetical protein from Mycobacterium leprae (147 aa), FASTA scores: opt: 778, E(): 0, (79.0% identity in 138 aa overlap). Also similar to hypothetical proteins from several Rickettsia species. P95198 No conserved hypotheticals 2FSX P95198 P95198 P95198 ML0276,ML0276c MMAR_0687 MSMEG_0768 Mb0396 NC_000962.3 Mycobrowser_v4 CDS 470010 471230 . + 0 Rv0391 metZ Involved in methionine biosynthesis. Converts O-succinylhomoserine into homocysteine. Probable O-succinylhomoserine sulfhydrylase MetZ (OSH sulfhydrylase) Rv0391, (MTCY04D9.03), len: 406 aa. Probable metZ, O-succinylhomoserine sulfhydrylase, equivalent, but shorter 20 aa in N-terminus, to AA18941.1|AL023514 O-succinylhomoserine sulfhydrylase from Mycobacterium leprae (426 aa). Also highly similar to others e.g. METZ_PSEAE|P55218 o-succinylhomoserine sulfhydrylase from Pseudomonas aeruginosa (403 aa), FASTA scores: opt: 1175, E(): 0, (47.2% identity in 392 aa overlap); etc. Belongs to the trans-sulfuration enzymes family. Could also be a cystathionine gamma-synthase. P9WGB5 No intermediary metabolism and respiration 3NDN P95199 P9WGB5 GO:0016829,GO:0030170,GO:0006520 4.2.99.- P9WGB5 ML0275,ML0275c MMAR_0688 MSMEG_0769 Mb0397 NC_000962.3 Mycobrowser_v4 CDS 471227 472639 . - 0 Rv0392c ndhA Transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Does not couple the redox reaction to proton translocation [catalytic activity: NADH + acceptor = NAD+ + reduced acceptor]. Probable membrane NADH dehydrogenase NdhA Rv0392c, (MTCY04D9.04c), len: 470 aa. Probable ndhA, membrane NADH dehydrogenase, equivalent to many e.g. AF038423|AF038423_1 NADH dehydrogenase from Mycobacterium smegmatis (457 aa), FASTA scores: opt: 1991, E(): 0, (67.9% identity in 458 aa overlap); MLCB1788_3 NADH dehydrogenase from Mycobacterium leprae (466 aa), FASTA score: (62.5% identity in 467 aa overlap). Also similar to others from several organisms e.g. P00393|DHNA_ECOLI|66211|581140|CAA23586.1|V00306 NADH dehydrogenase from Escherichia coli (434 aa); and Rv0392c|ndhB from Mycobacterium tuberculosis. Has hydrophobic stretch in C-terminus. Belongs to the NADH dehydrogenase family. P95200 No intermediary metabolism and respiration P95200 P95200 GO:0003954,GO:0055114,GO:0050660 1.6.99.3 P95200 MMAR_0689 Mb0398c NC_000962.3 Mycobrowser_v4 CDS 477327 478556 . - 0 Rv0399c lpqK Unknown Possible conserved lipoprotein LpqK Rv0399c, (MTCY04D9.11c), len: 409 aa. Possible lpqK, conserved lipoprotein, showing some similarity to penicillin binding proteins and various peptidases e.g. DAC_STRSQ|P15555 d-alanyl-d-alanine carboxypeptidase protein (406 aa), FASTA scores: opt: 348, E(): 5.6e-16, (29.2% identity in 301 aa overlap). Also similar to other Mycobacterium tuberculosis PBPs and esterases. Has possible N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein lipid attachment site (PS00013). P95207 No cell wall and cell processes P95207 P95207 P95207 MMAR_0696 Mb0405c NC_000962.3 Mycobrowser_v4 CDS 544835 545185 . + 0 Rv0454 Rv0454 Function unknown Conserved hypothetical protein Rv0454, (MTV037.18), len: 116 aa (start uncertain). Conserved hypothetical protein, showing similarity with AAA63007.1|U15183 hypothetical protein from Mycobacterium leprae (115 aa), FASTA scores: opt: 151, E(): 0.0019, (31.5% identity in 89 aa overlap). O86364 No conserved hypotheticals O86364 Mb0462 NC_000962.3 Mycobrowser_v4 CDS 480355 483231 . - 0 Rv0402c mmpL1 Unknown. Thought to be involved in fatty acid transport. Probable conserved transmembrane transport protein MmpL1 Rv0402c, (MTCY04D9.15c), len: 958 aa. Probable mmpL1, conserved transmembrane transport protein (see Tekaia et al., 1999), member of RND superfamily, highly similar to other Mycobacterial proteins e.g. YV34_MYCTU|Q11171 hypothetical 106.2 kDa membrane protein from Mycobacterium tuberculosis (968 aa), FASTA scores: opt: 3551, E(): 0, (55.4% identity in 933aa overlap); YV34_MYCLE|P54881 hypothetical 105.2 kDa protein from Mycobacterium leprae (959 aa), FASTA scores: opt: 3615, E(): 0, (55.5% identity in 941 aa overlap); etc. Highly similar to many other mycobacterial MmpL proteins from Mycobacterium tuberculosis and Mycobacterium leprae e.g. Rv0450c, Rv0676c, Rv0507, etc. Belongs to the MmpL family. P9WJV9 No cell wall and cell processes P95211 P9WJV9 GO:0005886,GO:0016021 MMAR_0704,MMAR_1722 Mb0408c,Mb0409c NC_000962.3 Mycobrowser_v4 CDS 483228 483656 . - 0 Rv0403c mmpS1 Unknown Probable conserved membrane protein MmpS1 Rv0403c, (MTCY04D9.16c), len: 142 aa. Probable mmpS1, conserved membrane protein (see citation below), highly similar to other Mycobacterial proteins e.g. YV33_MYCLE|P54880 hypothetical 16.9 kDa protein from Mycobacterium leprae (154 aa), FASTA scores: opt: 458, E(): 1.6e-26, (46.9% identity in 143 aa overlap); YV33_MYCTU|Q11170 hypothetical 15.9 kDa protein from Mycobacterium tuberculosis (147 aa), FASTA scores: opt: 362, E(): 1.1e-19, (42.1% identity in 140 aa overlap); etc. Also similar to other MmpS proteins from Mycobacterium tuberculosis e.g. Rv0677c, Rv0451c, etc. Belongs to the MmpS family. Predicted to be an outer membrane protein (See Song et al., 2008). P9WJT5 No cell wall and cell processes P0A5K0 P9WJT5 GO:0005886,GO:0016021 P9WJT5 MMAR_0705,MMAR_1723 Mb0410c NC_000962.3 Mycobrowser_v4 CDS 485731 489939 . + 0 Rv0405 pks6 Polyketide synthase possibly involved in lipid synthesis. Probable membrane bound polyketide synthase Pks6 Rv0405, (MTCY22G10.01), len: 1402 aa. Probable pks6, membrane-bound polyketide synthase (see citation below), highly similar to others e.g. CAC29643.1|AL583917 putative polyketide synthase from Mycobacterium leprae (2103 aa); Y06K_MYCTU|Q10977 probable polyketide synthase (1876 aa), FASTA scores: opt: 2303, E(): 0, (38.7% identity in 1232 aa overlap); etc. Contains PS00606 Beta-ketoacyl synthases active site, 2 x PS00017 ATP/GTP-binding site motif A (P-loop), and PS00012 Phosphopantetheine attachment site. O86335 No lipid metabolism O86335 O86335 GO:0009058,GO:0048037,GO:0016788,GO:0031177,GO:0016740,GO:0000036 O86335 Mb0412,Mb0413 NC_000962.3 Mycobrowser_v4 CDS 489887 490705 . - 0 Rv0406c Rv0406c Function unknown Beta lactamase like protein Rv0406c, (MTCY22G10.02c), len: 272 aa. Beta-lactamase-like protein, equivalent to AAD38170.1|AF152397_1 beta-lactamase-like protein from Mycobacterium phlei (243 aa); AL023514|MLCB4_8 hypothetical protein from Mycobacterium leprae (251 aa), FASTA scores: opt: 1284, E(): 0, (74.9% identity in 243 aa overlap); and AAD38164.1|AF152394_2 beta-lactamase-like protein from Mycobacterium avium (247 aa), FASTA scores: opt: 1301, E(): 0, (74.2% identity in 244 aa overlap); etc. Also slight similarity to others beta-lactamases and hypothetical proteins e.g. P52700|BLA1_XANMA|628530|S45349 metallo-beta-lactamase L1 precursor (beta-lactamase, type II) (penicillinase) from Xanthomonas maltophilia (290 aa), FASTA scores: (34.4% identity in 96 aa overlap). Recombinant protein has beta lactamase activity (See Nampoothiri et al., 2008). O86336 No conserved hypotheticals O86336 GO:0016787 O86336 ML0270 MMAR_0708 MSMEG_0775 Mb0414c NC_000962.3 Mycobrowser_v4 CDS 493851 495008 . + 0 Rv0409 ackA Involved at the first step (of two) in the conversion of acetate to acetyl-CoA [catalytic activity: ATP + acetate = ADP + acetyl phosphate]. Probable acetate kinase AckA (acetokinase) Rv0409, (MTCY22G10.05), len: 385 aa. Probable ackA, acetate kinase, highly similar to others e.g. ACKA_BACSU|P37877 acetate kinase from Bacillus subtilis (395 aa), FASTA scores: opt: 974, E(): 0, (43.5% identity in 393 aa overlap); etc. Contains PS01075 Acetate and butyrate kinases family signature 1, PS00758 ArgE / dapE / ACY1/ CPG2 / yscS family signature 1. Belongs to the acetokinase family. P9WQH1 No intermediary metabolism and respiration P63409 P9WQH1 GO:0008776,GO:0005737,GO:0006082,GO:0016310,GO:0005524 2.7.2.1 P9WQH1 ML0267,ML0267c MMAR_0711 MSMEG_0784 Mb0417 NC_000962.3 Mycobrowser_v4 CDS 497314 498300 . - 0 Rv0411c glnH Involved in active transport of glutamine across the membrane (import). Interacts with the glutamine-transport system. Probable glutamine-binding lipoprotein GlnH (GLNBP) Rv0411c, (MTCY22G10.07c), len: 328 aa. Probable glnH, glutamine-binding protein, membrane-bound lipoprotein (see citation below), equivalent to AL035159|MLCB1450_15|T44736|4154051|CAA22704.1 glutamine-binding protein homolog from Mycobacterium leprae (325 aa), FASTA scores: opt: 1747, E(): 0, (79.3% identity in 328 aa overlap). Also similar to others e.g. GLNH_BACST|P27676 glutamine-binding protein precursor from Bacillus stearothermophilus (262 aa), FASTA scores: opt: 493, E(): 7.5e-22, (37.8% identity in 193 aa overlap); etc. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site, PS01039 Bacterial extracellular solute-binding proteins, family 3 signature. Belongs to the bacterial extracellular solute-binding protein family 3. Presumed attached to the membrane by a lipid anchor. P96257 No cell wall and cell processes P96257 P96257 GO:0006810,GO:0005215,GO:0030288 P96257 ML0303 MMAR_0714 MSMEG_0787 Mb0419c NC_000962.3 Mycobrowser_v4 CDS 1241633 1241956 . + 0 Rv1117 Rv1117 Function unknown Conserved protein Rv1117, (MTCY22G8.06), len: 107 aa. Conserved protein, some similarity to P94425|D50453 hypothetical protein from Bacillus subtilis (95 aa), fasta scores: opt: 128, E(): 5.1e-06, (28.3% identity in 92 aa overlap); and AL117322|SCF1.02 Streptomyces coelicolor (109 aa), FASTA scores: opt: 437, E(): 1.6e-25, (57.5% identity in 106 aa overlap). O06569 No conserved hypotheticals O06569 O06569 GO:0005737,GO:0016491,GO:0017000 O06569 ML0941 MMAR_4343 MSMEG_1532 Mb1148 NC_000962.3 Mycobrowser_v4 CDS 495062 497314 . - 0 Rv0410c pknG Involved in signal transduction (via phosphorylation). Thought to regulate amino-acid uptake and stationary-phase metabolism. Phosphorylates the peptide substrate myelin basic protein (MBP) at serine residues [catalytic activity: ATP + a protein = ADP + a phosphoprotein]. Serine/threonine-protein kinase PknG (protein kinase G) (STPK G) Rv0410c, (MTCY22G10.06c), len: 750 aa. PknG, serine/threonine-protein kinase (see citations below), equivalent to PKNG_MYCLE|P57993|13092623|CAC29812.1|AL583918 probable serine/threonine-protein kinase from Mycobacterium leprae (767 aa). Also similar to others e.g. AB76890.1|AL159139 putative serine/threonine protein kinase from Streptomyces coelicolor (774 aa); etc. Contains PS00108 Serine/Threonine protein kinases active-site signature. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases. Structure of PknG with inhibitor AX20017 reveals that the inhibitor-binding pocket is shaped by a unique set of amino acid side chains not found in any human kinase (See Scherr et al., 2007). P9WI73 No regulatory proteins 2PZI P65728 P9WI73 GO:0006468,GO:0004674,GO:0005524 2.7.11.1 P9WI73 ML0304 MMAR_0713 MSMEG_0786 Mb0418c NC_000962.3 Mycobrowser_v4 CDS 501148 502170 . + 0 Rv0415 thiO Possibly involved in thiamine biosynthesis. Possible thiamine biosynthesis oxidoreductase ThiO Rv0415, (MTCY22G10.12), len: 340 aa. Possible thiO, thiamine biosynthesis oxidoreductase, equivalent to T44739|4154054|CAA22708.1|AL035159|MLCB1450.24 hypothetical protein from Mycobacterium leprae (340 aa), FASTA scores: opt: 1867, E(): 0, (82.0% identity in 338 aa overlap). Shows some similarity to other thiO proteins e.g. THIO_RHIET|O34292 Putative thiamine biosynthesis oxidoreductase from Rhizobium etli plasmid pb (327 aa) (see citation below); AAG31046.1|AF264948_8|THIO putative amino acid oxidase flavoprotein ThiO from Erwinia amylovora (349 aa); NP_106392.1|14025578|BAB52178.1|AP003007|THIO thiamine biosynthesis oxidoreductase THIO from Mesorhizobium loti (333 aa); etc. P96261 No intermediary metabolism and respiration P96261 P96261 GO:0055114,GO:0016491,GO:0050660 1.-.-.- P96261 ML0299,ML0299c MMAR_0718 MSMEG_0791 Mb0423 NC_000962.3 Mycobrowser_v4 CDS 502167 502373 . + 0 Rv0416 thiS Possibly involved in thiamine biosynthesis. Possible protein ThiS Rv0416, (MTCY22G10.13), len: 68 aa. Possible thiS protein, equivalent to T44740|4154055|CAA22709.1|AL035159|MLCB1450.25 hypothetical protein from Mycobacterium leprae (74 aa), FASTA scores: opt: 303, E(): 2e-18, (71.6% identity in 74 aa overlap). Shows weak similarity with O32583|THIS_ECOLI|THIG1|B3991.1 this protein from Escherichia coli strain K12 (66 aa), FASTA scores: opt: 103, E(): 0.052, (30.9% identity in 68 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P96262 No intermediary metabolism and respiration P96262 P96262 GO:0006790 P96262 ML0298,ML0298c MMAR_0719 MSMEG_0792 Mb0424 NC_000962.3 Mycobrowser_v4 CDS 545889 546803 . - 0 Rv0456c echA2 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. enoyl-CoA hydratase EchA2 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv0456c, (MTCI429A.02, MTV037.20c), len: 304 aa. Probable echA2, enoyl-CoA hydratase, similar to other enoyl-CoA hydratases e.g. Q13011 peroxisomal enoyl-CoA hydratase-like protein (328 aa), FASTA scores: opt: 209, E(): 5.3e-07, (31.7% identity in 142 aa overlap). Also similar to several other proteins from Mycobacterium tuberculosis e.g. MTCY09F9.29 FASTA score: (32.9% identity in 146 aa overlap); and MTI376.01c. O07179 No lipid metabolism O07179 O07179 GO:0016853,GO:0008152,GO:0004300 4.2.1.17 O07179 ML2381,ML2381c MMAR_0778 Mb0464c NC_000962.3 Mycobrowser_v4 CDS 1592150 1592614 . + 0 Rv1417 Rv1417 Unknown Possible conserved membrane protein Rv1417, (MTCY21B4.35), len: 154 aa. Possible conserved membrane protein, similar to others e.g. AL133213|SC6D7_2 Streptomyces coelicolor (156 aa), FASTA scores: opt: 212, E(): 4.4e-07, (32.4% identity in 136 aa overlap). P9WLY1 No cell wall and cell processes P64847 P9WLY1 GO:0005886,GO:0016021 ML0561 MMAR_2224 MSMEG_3074 Mb1452 NC_000962.3 Mycobrowser_v4 CDS 505086 506582 . + 0 Rv0419 lpqM Function unknown; possibly hydrolyzes peptides and/or proteins. Possible lipoprotein peptidase LpqM Rv0419, (MTCY22G10.16), len: 498 aa. Possible lpqM, lipoprotein peptidase ; has potential N-terminal signal peptide and contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site, PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. P96265 No cell wall and cell processes P96265 GO:0004177 3.4.11.- P96265 MMAR_0728 MSMEG_4893,MSMEG_4913 NC_000962.3 Mycobrowser_v4 CDS 506561 506971 . - 0 Rv0420c Rv0420c Unknown Possible transmembrane protein Rv0420c, (MTCY22G10.17c), len: 136 aa. Possible transmembrane protein; has potential transmembrane domains aa 53-99 and aa 100-122. P96266 No cell wall and cell processes P96266 GO:0016021 MMAR_0731 MSMEG_0813 Mb0428c NC_000962.3 Mycobrowser_v4 CDS 507132 507761 . - 0 Rv0421c Rv0421c Function unknown Conserved hypothetical protein Rv0421c, (MTCY22G10.18c), len: 209 aa. Conserved hypothetical protein, showing similarity with NP_103507.1|14022684|BAB49293.1|AP002998 hypothetical protein from Mesorhizobium loti (214 aa). P96267 No conserved hypotheticals P96267 P96267 ML0296 MMAR_0733 MSMEG_0824 Mb0429c NC_000962.3 Mycobrowser_v4 CDS 507758 508555 . - 0 Rv0422c thiD Involved in thiamine biosynthesis. Catalyzes the phosphorylation of HMP-P to HMP-pp [catalytic activity: ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine]. Probable phosphomethylpyrimidine kinase ThiD (HMP-phosphate kinase) (HMP-P kinase) Rv0422c, (MTCY22G10.19c), len: 265 aa. Probable thiD, phosphomethylpyrimidine kinase, equivalent to AL035159|MLCB1450_21 phosphomethylpyrimidine kinase from Mycobacterium leprae (279 aa), FASTA scores: opt: 1386, E(): 0, (77.8% identity in 266 aa overlap). Also highly similar to others e.g. HIU32725_3|P44697|THID_HAEIN phosphomethylpyrimidine kinase from Haemophilus influenzae (269 aa), FASTA scores: opt: 605, E(): 0, (42.1% identity in 259 aa overlap). Belongs to the ThiD family. P9WG77 No intermediary metabolism and respiration P66913 P9WG77 GO:0008902,GO:0008972,GO:0009228,GO:0005524 2.7.4.7,2.7.1.49 P9WG77 ML0295 MMAR_0734 MSMEG_0825 Mb0430c NC_000962.3 Mycobrowser_v4 CDS 510377 510652 . - 0 Rv0424c Rv0424c Unknown Hypothetical protein Rv0424c, (MTCY22G10.21c), len: 91 aa. Hypothetical unknown protein. P96270 No conserved hypotheticals P96270 P96270 ML1934 Mb0432c NC_000962.3 Mycobrowser_v4 CDS 596759 597202 . + 0 Rv0506 mmpS2 Unknown Probable conserved membrane protein MmpS2 Rv0506, (MTCY20G9.33), len: 147 aa. Probable mmpS2, conserved membrane protein (see citation below), highly similar to other Mycobacterial proteins e.g. C-terminus of AAD44232.1|AF143772_38|AF143772|TmtpA from Mycobacterium avium (221 aa); P54880|MMS4_MYCLE|MMPS4 putative membrane protein from Mycobacterium leprae (154 aa), FASTA scores: opt: 392, E(): 1.3e-20, (43.7% identity in 151 aa overlap); and the putative membrane proteins from Mycobacterium tuberculosis MTV040_5, MTCY4D9_16, MTV037_15. Belongs to the MmpS family. Predicted to be an outer membrane protein (See Song et al., 2008). P9WJT3 No cell wall and cell processes P65376 P9WJT3 GO:0016021 P9WJT3 MMAR_0839 MSMEG_0186 Mb0518 NC_000962.3 Mycobrowser_v4 CDS 1592639 1593325 . + 0 Rv1418 lprH Unknown Probable lipoprotein LprH Rv1418, (MTCY21B4.36), len: 228 aa. Probable lprH, lipoprotein. Contains N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein lipid attachment site (PS00013). P9WK43 No cell wall and cell processes P9WK43 GO:0005886,GO:0016021 P9WK43 MMAR_2225 Mb1453 NC_000962.3 Mycobrowser_v4 CDS 2370598 2370792 . - 0 Rv2111c pup Involved in proteasomal degradation. Covalently binds to protein substrates. Prokaryotic ubiquitin-like protein Pup Rv2111c, MTCY261.07c, len: 64 aa. Pup, prokaryotic ubiquitin-like protein (See Pearce et al., 2008). Highly similar to many. Pup|Rv2111c and Mpa|Rv2115c interact (See Pearce et al., 2008). P9WHN5 No intermediary metabolism and respiration 3M91,3M9D O33246 P9WHN5 GO:0070490,GO:0019941 P9WHN5 ML1321 MMAR_3086 MSMEG_3896 Mb2135c NC_000962.3 Mycobrowser_v4 CDS 515373 515816 . - 0 Rv0426c Rv0426c Unknown Possible transmembrane protein Rv0426c, (MTCY22G10.23c), len: 147 aa. Possible transmembrane protein; has potential transmembrane domains aa 19-41, and aa 61-83. P96272 No cell wall and cell processes P96272 GO:0016021 ML1932 Mb0434c NC_000962.3 Mycobrowser_v4 CDS 517803 518396 . - 0 Rv0429c def Removes the formyl group from the N-terminal met of newly synthesized proteins [catalytic activity: N-formyl-L-methionine + H2O = formate + L-methionine]. Probable polypeptide deformylase Def (PDF) (formylmethionine deformylase) Rv0429c, (MTCY22G10.26c), len: 197 aa. Probable def, polypeptide deformylase, equivalent to CAC30884.1|AL583923 polypeptide deformylase from Mycobacterium leprae (197 aa). Also similar to others e.g. DEF_ECOLI|P27251|95874|S23107 polypeptide deformylase from Escherichia coli (169 aa), FASTA scores: opt: 179, E(): 1.8e-05, (34.6% identity in 162 aa overlap); etc. Belongs to the polypeptide deformylase family. Cofactor: binds 1 zinc ion. P9WIJ3 No information pathways 3E3U P96275 P9WIJ3 GO:0042586,GO:0006412,GO:0005506 3.5.1.88 P9WIJ3 ML1929 MMAR_0744 MSMEG_0832 Mb0437c NC_000962.3 Mycobrowser_v4 CDS 518733 519041 . + 0 Rv0430 Rv0430 Function unknown Conserved hypothetical protein Rv0430, (MTCY22G10.27), len: 102 aa. Conserved hypothetical protein, equivalent to AC30882.1|AL583923 conserved hypothetical protein from Mycobacterium leprae (102 aa). Also highly similar to CAB93047.1|SCD95A.20|AL357432 hypothetical protein from Streptomyces coelicolor (84 aa). P96276 No conserved hypotheticals P96276 P96276 ML1927,ML1927c Mb0438 NC_000962.3 Mycobrowser_v4 CDS 519073 519567 . + 0 Rv0431 Rv0431 Unknown Putative tuberculin related peptide Rv0431, (MTCY22G10.28), len: 164 aa. Putative tuberculin related peptide; almost identical to D00815|MSGAT103_1 AT103 from Mycobacterium tuberculosis (172 aa), FASTA score: (99.4% identity in 163 aa overlap). Highly similar to to CAC30881.1|AL583923 tuberculin related peptide (AT103) from Mycobacterium leprae (167 aa). Some similarity to G550415|HRPC (282 aa), FASTA scores: opt: 120, E(): 0.36, (33.3% identity in 111 aa overlap). Potential transmembrane domain at aa 19-37. P96277 No cell wall and cell processes P96277 P96277 ML1926,ML1926c MMAR_0746 MSMEG_0834 Mb0439 NC_000962.3 Mycobrowser_v4 CDS 519600 520322 . + 0 Rv0432 sodC Destroys radicals which are normally produced within the cells and are toxic to biological systems [catalytic activity: 2 superoxide + 2 H+ = O2 + H2O2]. Periplasmic superoxide dismutase [Cu-Zn] SodC Rv0432, (MTCY22G10.29), len: 240 aa. sodC, periplasmic superoxide dismutase [Cu-Zn], equivalent to CAC30880.1|AL583923 superoxide dismutase precursor (Cu-Zn) from Mycobacterium leprae (240 aa); and AAK20038.1|AF326234_1 copper zinc superoxide dismutase from Mycobacterium avium subsp. paratuberculosis (226 aa). Also similar to others e.g. SODC_PHOLE|P00446 superoxide dismutase precursor (Cu-Zn) from Photobacterium leiognathi (173 aa), FASTA scores: opt: 214, E(): 5.2 e-06, (36.5% identity in 181 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Belongs to the Cu-Zn superoxide dismutase family. Possibly localized in periplasm, membrane-bound. P9WGE9 No virulence, detoxification, adaptation 1PZS P0A608 P9WGE9 GO:0055114,GO:0005886,GO:0004784,GO:0006801,GO:0046872 1.15.1.1 P9WGE9 ML1925,ML1925c MMAR_0747 MSMEG_0835 Mb0440 NC_000962.3 Mycobrowser_v4 CDS 1901047 1901751 . + 0 Rv1676 Rv1676 Unknown Unknown protein Rv1676, (MTV047.11), len: 234 aa. Unknown protein. O53923 No conserved hypotheticals O53923 O53923 GO:0016491,GO:0016209 O53923 MMAR_2967 MSMEG_2582 Mb1703 NC_000962.3 Mycobrowser_v4 CDS 524530 525390 . - 0 Rv0436c pssA Involved in phospholipid biosynthesis. Generates phosphatidylserine [catalytic activity: CDP-diacylglycerol + L-serine = CMP + O-SN-phosphatidyl-L-serine]. Probable CDP-diacylglycerol--serine O-phosphatidyltransferase PssA (PS synthase) (phosphatidylserine synthase) Rv0436c, (MTCY22G10.33c), len: 286 aa. Probable pssA, PS synthase (CDP-diacylglycerol--serine O-phosphatidyltransferase) (see citation below), integral membrane protein, equivalent to AL035159|MLCB1450_9|T44730 from Mycobacterium leprae (300 aa), FASTA scores: opt: 1506, E(): 0, (77.9% identity in 285 aa overlap). Also highly similar to others e.g. NP_108059.1|14027250|BAB54204.1|AP003012 phosphatidylserine synthase from Mesorhizobium loti (248 aa); PSS_BACSU|P39823 cdp-diacylglycerol--serine o-phosphatidyltransferase from Bacillus subtilis (177 aa), FASTA scores: opt: 277, E(): 9.9e-12, (33.3% identity in 183 aa overlap); etc. Contains PS00379 CDP-alcohol phosphatidyltransferases signature. Belongs to the CDP-alcohol phosphatidyltransferase class-I family. P9WPG1 No lipid metabolism P96282 P9WPG1 GO:0003882,GO:0016021,GO:0008654,GO:0005886,GO:0046341 2.7.8.8 ML0310,ML0310c MMAR_0753 MSMEG_0860 Mb0444c NC_000962.3 Mycobrowser_v4 CDS 526143 527360 . - 0 Rv0438c moeA2 Involved in molybdenum cofactor biosynthesis: involved in the biosynthesis of a demolybdo-cofactor (molybdopterin), necessary for molybdo-enzymes. Probable molybdopterin biosynthesis protein MoeA2 Rv0438c, (MTV037.02c), len: 405 aa. Probable moeA2, molybdenum cofactor biosynthesis protein, highly similar to many e.g. Y10817|ANY10817_2 from A. nicotinovorans (429 aa), FASTA scores: opt: 786, E(): 0, (39.2% identity in 398 aa overlap); etc. Also similar to MOEA1|Rv0994|MTCI237.08|O05577 probable molybdopterin biosynthesis protein from Mycobacterium tuberculosis (426 aa), FASTA scores: opt: 667, E(): 2e-32, (36.5% identity in 425 aa overlap). Note that previously known as moeA3. P9WJQ5 No intermediary metabolism and respiration O53725 P9WJQ5 GO:0046872,GO:0016740,GO:0006777 2.-.-.- P9WJQ5 ML0312,ML0312c MMAR_0756 MSMEG_0862 Mb0446c NC_000962.3 Mycobrowser_v4 CDS 554313 554885 . - 0 Rv0464c Rv0464c Function unknown Conserved protein Rv0464c, (MTV038.08c), len: 190 aa. Conserved protein, highly similar to CAC31982.1|AL583925 conserved hypothetical protein from Mycobacterium leprae (188 aa). Also some similarity with Rv1531|AL022000|MTV045_5|D70820 hypothetical protein from Mycobacterium tuberculosis (188 aa), FASTA scores: E(): 9.6e-10, (30.9% identity in 175 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53749 No conserved hypotheticals O53749 O53749 O53749 ML2465 MMAR_0789 MSMEG_0905 Mb0473c NC_000962.3 Mycobrowser_v4 CDS 3964479 3965192 . - 0 Rv3528c Rv3528c Unknown Unknown protein Rv3528c, (MTCY03C7.28), len: 237 aa. Unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6YGE4 No conserved hypotheticals I6YGE4 I6YGE4 Mb3558c NC_000962.3 Mycobrowser_v4 CDS 530296 530724 . - 0 Rv0441c Rv0441c Unknown Hypothetical protein Rv0441c, (MTV037.05c), len: 142 aa. Hypothetical unknown protein. P9WKW3 No conserved hypotheticals P9WKW3 P9WKW3 MMAR_0760 MSMEG_0882 Mb0449c NC_000962.3 Mycobrowser_v4 CDS 530751 532214 . - 0 Rv0442c PPE10 Function unknown PPE family protein PPE10 Rv0442c, (MTV037.06c), len: 487 aa. PPE10, Member of the Mycobacterium tuberculosis PPE family, nearly identical to hypothetical protein from Mycobacterium tuberculosis (strain Erdman) and to AN5S46909_1 protein fragment from Mycobacterium bovis (302 aa); P42611|YHS6_MYCTU hypothetical 50.6 kDa protein (517 aa), FASTA scores: opt: 3144, E(): 0, (98.4 identity in 492 aa overlap); and S46909|S46909_1 (302 aa), FASTA scores: opt: 1897, E(): 0, (98.0% identity in 302 aa overlap). Nucleotide position 532097 in the genome sequence has been corrected, T:C resulting in K40E. P9WI41 No PE/PPE P42611 P9WI41 P9WI41 ML2369c Mb0450c NC_000962.3 Mycobrowser_v4 CDS 547076 547357 . - 0 Rv0456A mazF1 Function unknown Possible toxin MazF1 Rv0456A, len: 93 aa. Possible mazF1, toxin, part of toxin-antitoxin (TA) operon with Rv0456B (See Pandey and Gerdes, 2005; Zhu et al., 2006); N-terminus highly similar to N-terminal part of P71650|Rv2801c|MT2869|MTCY16B7.42 conserved hypothetical protein from Mycobacterium tuberculosis (118 aa), FASTA scores: opt: 303, E(): 1e-14, (60.44% identity in 91 aa overlap). Also some similarity in part with other hypothetical proteins e.g. Q9PHH8|XFA0027 Plasmid maintenance protein from Xylella fastidiosa (108 aa), FASTA scores: opt: 169, E(): 3.9e-05, (50.820% identity in 61 aa overlap). Q6MX40 No virulence, detoxification, adaptation Q6MX40 Q6MX40 GO:0003677 Q6MX40 Mb0465c NC_000962.3 Mycobrowser_v4 CDS 533091 533789 . - 0 Rv0444c rskA Regulates negatively Rv0445c|SIGK Anti-sigma factor RskA (regulator of sigma K) Rv0444c, (MTV037.08c), len: 232 aa. RskA, regulator of SigK (See Said-Salim et al., 2006); C-terminus similar to P12752|Y24K_STRGR hypothetical 24.7 kDa protein from Streptomyces griseus (238 aa), FASTA scores: opt: 207, E(): 2.2e-05, (32.9% identity in 158 aa overlap). Cleaved by Rip|Rv2869c, in M. tuberculosis Erdman (See Sklar et al., 2010). P9WGX5 No information pathways O53729 P9WGX5 GO:0005886,GO:0016021 P9WGX5 MMAR_0765 Mb0452c NC_000962.3 Mycobrowser_v4 CDS 552614 554008 . + 0 Rv0462 lpdC Involved in energy metabolism. Lipoamide dehydrogenase is the E3 component of pyruvate dehydrogenase and the alpha-ketoacid dehydrogenase complex [catalytic activity: dihydrolipoamide + NAD(+) = lipoamide + NADH]. Also involved in antioxidant defense; LPDC|Rv0462, DLAT|Rv2215, AHPD|Rv2429, and AHPC|Rv2428 constitute an NADH-dependent peroxidase and peroxynitrite reductase that provides protection against oxidative stress. Dihydrolipoamide dehydrogenase LpdC (lipoamide reductase (NADH)) (lipoyl dehydrogenase) (dihydrolipoyl dehydrogenase) (diaphorase) Rv0462, (MTV038.06), len: 464 aa. LpdC (alternate gene name: TB49.2, CIP50), dihydrolipoamide dehydrogenase (see Argyrou & Blanchard 2001), equivalent to AAA63016.1|U15183 lipoamide dehydrogenase from Mycobacterium leprae (467 aa), FASTA scores: opt: 2583, E(): 0, (83.1% identity in 467 aa overlap). Also similar to to many e.g. P50970|DLDH_ZYMMO|X82291|ZMLPD_1 dihydrolipoamide dehydrogenase from Z.mobilis (466 aa), FASTA scores: opt: 1198, E(): 0, (42.4 % identity in 465 aa overlap); etc. Belongs to the pyridine nucleotide-disulfide oxidoreductases class-I. Binds to coronin-1 in BCG and M. tuberculosis - coronin-1 is retained on phagosomes and phagosome maturation is arrested (See Deghmane et al., 2007). LpdC|Rv0462 co-immunoprecipitates with DlaT|Rv2215 (in lpdC|Rv0462 mutant) and with BkdC|Rv2495c (in dlaT|Rv2215 mutant) (See Venugopal et al., 2011). P9WHH9 No intermediary metabolism and respiration 2A8X,3II4 P66004 P9WHH9 GO:0016209,GO:0045454,GO:0005737,GO:0004148,GO:0006096,GO:0055114,GO:0050660 1.8.1.4 P9WHH9 ML2387 MMAR_0785 MSMEG_0903 Mb0471 NC_000962.3 Mycobrowser_v4 CDS 1051544 1052317 . - 0 Rv0941c Rv0941c Function unknown Conserved hypothetical protein Rv0941c, (MTCY10D7.33), len: 257 aa. Conserved hypothetical protein, showing some similarity with parts of several hypothetical proteins from Streptomyces coelicolor e.g. AL035161|SC9C7_20 (860 aa), FASTA scores: opt: 197, E(): 2.6e-05, (34.2% identity in 114 aa overlap). P71568 No conserved hypotheticals P71568 P71568 P71568 ML0146,ML0146c MMAR_4567 Mb0966c NC_000962.3 Mycobrowser_v4 CDS 534445 535215 . - 0 Rv0446c Rv0446c Unknown Possible conserved transmembrane protein Rv0446c, (MTV037.10c), len: 256 aa. Possible conserved transmembrane protein, similar at N-terminus to U1740AF|U15183|MLU15183_40 from Mycobacterium leprae (117 aa), FASTA scores: opt: 175, E(): 2.5e-05, (62.5% identity in 40 aa overlap); and at C-terminus to AL021529|SC10A5_3 from Streptomyces coelicolor (226 aa), FASTA scores: opt: 207, E(): 9.8e-07, (34.2% identity in 114 aa overlap). Also similar to others hypothetical proteins e.g. AAK04680.1|AE006291_14|AE006291 hypothetical protein from Lactococcus lactis subsp. lactis (257 aa). O53731 No cell wall and cell processes O53731 O53731 GO:0016021,GO:0006629,GO:0016627,GO:0005737 O53731 ML2371,ML2371c MMAR_0767 Mb0454c NC_000962.3 Mycobrowser_v4 CDS 536504 537169 . - 0 Rv0448c Rv0448c Function unknown Conserved hypothetical protein Rv0448c, (MTV037.12c), len: 221 aa. Conserved hypothetical protein, similar to other hypothetical proteins e.g. Z74841|BOD5A2_1 from B. oleracea (283 aa), FASTA scores: opt: 257, E(): 1.4e-10, (32.0% identity in 197 aa overlap); etc. Some similarity to U15183|MLU15183_38 from Mycobacterium leprae (82 aa), FASTA scores: opt: 134, E(): 0.014, (71.0% identity in 31 aa overlap). O53733 No conserved hypotheticals O53733 O53733 ML2373,ML2373c MMAR_0769 Mb0456c NC_000962.3 Mycobrowser_v4 CDS 537229 538548 . - 0 Rv0449c Rv0449c Function unknown Conserved hypothetical protein Rv0449c, (MTV037.13c), len: 439 aa. Conserved hypothetical protein, some similarity with several hypothetical proteins and various enzymes e.g. AAK24569.1|AE005927 amine oxidase, flavin-containing from Caulobacter crescentus (454 aa); BAB02771.1|AB023036 mycolic acid methyl transferase-like protein from Arabidopsis thaliana (842 aa); BAB01742.1|AP000374 protein which contains similarity to cyclopropane fatty acid synthase from Arabidopsis thaliana (793 aa); etc. Has hydrophobic stretch at N-terminus. O53734 No conserved hypotheticals O53734 O53734 O53734 MMAR_0770 Mb0457c NC_000962.3 Mycobrowser_v4 CDS 543174 544730 . + 0 Rv0453 PPE11 Function unknown PPE family protein PPE11 Rv0453, (MTV037.17), len: 518 aa. PPE11, Member of the Mycobacterium tuberculosis PPE family, similar to many e.g. AL0212|MTV012_32 from Mycobacterium tuberculosis (434 aa), FASTA scores: opt: 882, E(): 7e-31, (41.8% identity in 514 aa overlap). P9WI39 No PE/PPE Q6MX41 P9WI39 P9WI39 Mb0461 NC_000962.3 Mycobrowser_v4 CDS 632372 633034 . + 0 Rv0540 Rv0540 Function unknown Conserved hypothetical protein Rv0540, (MTCY25D10.19), len: 220 aa. Conserved hypothetical protein, similar to hypothetical proteins from Streptomyces coelicolor: CAB76990.1|AL159178 (213 aa); N-terminus of BAA84086.1|AB032065 (446 aa); and CAB61872.1|AL133252|SCE46_21 (210 aa), FASTA scores: opt: 267, E(): 5.3e-10, (32.7% identity in 202 aa overlap). Also some similarity with D90913_63|PCC6803 from Synecho cystis sp (211 aa), FASTA scores: opt: 189, E(): 4.7e-06, (25.3 identity in 194 aa overlap). O06406 No conserved hypotheticals O06406 O06406 O06406 MMAR_0886 MSMEG_0996 Mb0554 NC_000962.3 Mycobrowser_v4 CDS 541488 541910 . - 0 Rv0451c mmpS4 Unknown Probable conserved membrane protein MmpS4 Rv0451c, (MTV037.15c), len: 140 aa. Probable mmpS4, conserved membrane protein (see citations below), equivalent to U1740W|P54880|YV33_MYCLE hypothetical 16.9 kDa protein from Mycobacterium leprae (154 aa), FASTA scores: opt: 727, E(): 0, (75.9% identity in 137 aa overlap). Also similar to other Mycobacterial proteins e.g. Z84725|MTCY04D9.16c from Mycobacterium tuberculosis (142 aa), FASTA scores: opt: 451, E(): 3.2e-24, (50.0% identity in 138 aa overlap); etc. Belongs to the MmpS family. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). P9WJS9 No cell wall and cell processes P0A5K2 P9WJS9 GO:0016021 P9WJS9 ML2377 MMAR_0772 MSMEG_0380 Mb0459c NC_000962.3 Mycobrowser_v4 CDS 542142 542852 . + 0 Rv0452 Rv0452 Possibly involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0452, (MTV037.16), len: 236 aa. Possible transcriptional regulator, similar to several putative TetR-family transcriptional regulators from Streptomyces coelicolor. Also similar in N-terminus to U1740Y|U15183|MLU15183_33 from Mycobacterium leprae (67 aa), FASTA score: (76.1% identity in 67 aa overlap). Contains probable helix-turn-helix motif at aa 44-65 (Score 1727, +5.07 SD). O53737 No regulatory proteins O53737 O53737 GO:0006350,GO:0003700,GO:0006355 O53737 ML2376,ML2376c MMAR_0773 Mb0460 NC_000962.3 Mycobrowser_v4 CDS 547586 549607 . - 0 Rv0457c Rv0457c Function unknown; hydrolyzes peptides and/or proteins. Probable peptidase Rv0457c, (MTCI429A.01, MTV038.01c), len: 673 aa. Probable peptidase, similar to many e.g. NP_102851.1|14022026|BAB48637.1 probable endopeptidase from Mesorhizobium loti (687 aa); Y4NA_RHISN|P55577 probable peptidase (726 aa), FASTA scores: opt: 1126, E(): 0, (40.9% identity in 491 aa overlap). Also similar to Mycobacterium tuberculosis protein MTCY369.26 FASTA score: (33.8% identity in 299 aa overlap). O07178 No intermediary metabolism and respiration O07178 O07178 GO:0004252,GO:0006508 3.4.-.- O07178 ML2382,ML2382c MMAR_0779 MSMEG_0899 Mb0466c NC_000962.3 Mycobrowser_v4 CDS 549675 551198 . + 0 Rv0458 Rv0458 Interconversion aldehyde and acid [catalytic activity: an aldehyde + NAD+ + H2O = an acid + NADH]. Probable aldehyde dehydrogenase Rv0458, (MTV038.02), len: 507 aa. Probable aldehyde dehydrogenase, highly similar to many, closest to P46369|THCA_RHOER EPTC-inducible aldehyde dehydrogenase from Rhodococcus erythropolis (506 aa), FASTA scores: opt: 2767, E(): 0, (79.7% identity in 507 aa overlap); AAC13641.1|AF029733 chloroacetaldehyde dehydrogenase from Xanthobacter autotrophicus (505 aa), FASTA scores: opt: 2563, E(): 0, (75.4% identity in 492 aa overlap); Q9RJZ6|DHAL_STRCO probable aldehyde dehydrogenase from Streptomyces coelicolor (507 aa). Also similar to other semialdehyde dehydrogenases in Mycobacterium tuberculosis e.g. Rv0768, Rv2858c. Belongs to the aldehyde dehydrogenases family. P9WNY1 No intermediary metabolism and respiration P63937 P9WNY1 GO:0055114,GO:0004029 1.2.1.3 P9WNY1 ML2383 MMAR_0780 Mb0467 NC_000962.3 Mycobrowser_v4 CDS 551198 551689 . + 0 Rv0459 Rv0459 Function unknown Conserved hypothetical protein Rv0459, (MTV038.03), len: 163 aa. Conserved hypothetical protein, highly similar to other hypothetical proteins. Note that highly similar to products of unidentified ORFs in Xanthobacter autotrophicus, AF029733_2 (139 aa), and Rhodococcus erythropolis, REREUTP BC_1 (186 aa). Like MTV038.03, these ORF's are linked to aldehyde dehydrogenase genes. FASTA scores: AF0297|AF029733_2 (139 aa), opt: 439, E(): 6.2e-24, (50.0% identity in 126 aa overlap); and L24492|REREUTPBC_1 (186 aa), opt: 347, E(): 2.1e-17, (52.7% identity in 169 aa overlap). N-terminus also highly similar to AAA63041.1|U15183 ethanolamine permease (eutP) match from Mycobacterium leprae (53 aa). O53744 No conserved hypotheticals O53744 O53744 ML2384 MMAR_0781 MSMEG_0901 Mb0468 NC_000962.3 Mycobrowser_v4 CDS 551749 551988 . + 0 Rv0460 Rv0460 Function unknown Conserved hydrophobic protein Rv0460, (MTV038.04), len: 79 aa. Conserved hydrophobic protein, highly similar AAA63024.1|U15183 hypothetical protein from Mycobacterium leprae (56 aa), FASTA scores: opt: 197, E(): 3.7e-09, (63.8% identity in 47 aa overlap). O53745 No conserved hypotheticals O53745 ML2385 Mb0469 NC_000962.3 Mycobrowser_v4 CDS 556458 557252 . + 0 Rv0466 Rv0466 Function unknown Conserved protein Rv0466, (MTV038.10), len: 264 aa. Conserved protein, equivalent to CAC31980.1|AL583925 conserved hypothetical protein from Mycobacterium leprae (264 aa). Similar to Rv2001|Z74025|MTCY39.17c hypothetical 28.7 KDA protein from Mycobacterium tuberculosis (250 aa), FASTA scores: opt: 592, E(): 0, (38.0% identity in 263 aa overlap). Some similarity to several thioesterases e.g. Q42561|ATACPTE17_1 acyl-(acyl carrier protein) thioester from A. thaliana (362 aa), FASTA scores: E(): 0.0092, (24.4% identity in 197 aa overlap). O53751 No conserved hypotheticals O53751 O53751 O53751 ML2463,ML2463c MMAR_0791 MSMEG_0909 Mb0475 NC_000962.3 Mycobrowser_v4 CDS 558895 559755 . + 0 Rv0468 fadB2 Butyrate/butanol-producing pathway [catalytic activity: (S)-3-hydroxybutanoyl-CoA + NADP+ = 3-acetoacetyl-CoA + NADPH] 3-hydroxybutyryl-CoA dehydrogenase FadB2 (beta-hydroxybutyryl-CoA dehydrogenase) (BHBD) Rv0468, (MTV038.12), len: 286 aa. fadB2, 3-hydroxybutyryl-CoA dehydrogenase, equivalent to CAC31978.1|AL583925 3-hydroxyacyl-CoA dehydrogenase from Mycobacterium leprae (287 aa). Also similar to many 3-hydroxybutyryl-CoA dehydrogenases e.g. U32229|BJU32229_1 beta-hydroxybutyryl coenzyme A dehydrogenase from Bradyrhizobium japonicum (293 aa), FASTA scores: opt: 771, E(): 0, (45.7% identity in 282 aa overlap). Belongs to the 3-hydroxyacyl-CoA dehydrogenase family. P9WNP7 No lipid metabolism O53753 P9WNP7 GO:0050662,GO:0006631,GO:0055114,GO:0008691 1.1.1.157 P9WNP7 ML2461,ML2461c MMAR_0793 MSMEG_0912 Mb0477 NC_000962.3 Mycobrowser_v4 CDS 559888 560748 . + 0 Rv0469 umaA Involved in mycolic acid modification or synthesis. Possible mycolic acid synthase UmaA Rv0469, (MTV038.13), len: 286 aa. Possible umaA, mycolic acid synthase (see citations below), highly similar to CAC30854.1|AL583923 methyl mycolic acid synthase 1 from Mycobacterium leprae (286 aa); and CAC31976.1|AL583925 Mycolic acid synthase from Mycobacterium leprae (295 aa), FASTA scores: opt: 1402, E(): 0, (69.6% identity in 286 aa overlap). Also very similar to mycobacterial methyltransferases e.g. U77466|CmaD|MBU77466_1 (286 aa); MTCY20H10.26c|Z92772|MTY20H10_27 (296 aa); highly similar to CFA1_MYCTU|Q11195|U66108|MTU66108_1 cyclopropane-fatty-acyl-phospholipid synthase 1 (287 aa), FASTA scores: opt: 1360, E(): 0, (67.8% identity in 286 aa overlap) (see citation below); and very similar also to methoxy mycolic acid synthase 1 from Mycobacterium tuberculosis e.g. MTU66108_1 (286 aa). Note that previously known as umaA1. Q6MX39 No lipid metabolism Q6MX39 Q6MX39 GO:0008610,GO:0008825 2.-.-.- Q6MX39 ML2460,ML2460c MMAR_0794 MSMEG_0913 Mb0478 NC_000962.3 Mycobrowser_v4 CDS 566508 566771 . + 0 Rv0476 Rv0476 Unknown Possible conserved transmembrane protein Rv0476, (MTCY20G9.02), len: 87 aa. Possible conserved transmembrane protein, equivalent to CAC31970.1|AL583925 conserved membrane protein from Mycobacterium leprae (95 aa). Also highly similar to CAC04036.1|AL391406 putative membrane protein from Streptomyces coelicolor (113 aa). Contains PS00606 Beta-ketoacyl synthases active site. P9WKW1 No cell wall and cell processes P64695 P9WKW1 GO:0016021 ML2453,ML2453c Mb0486 NC_000962.3 Mycobrowser_v4 CDS 1108172 1108504 . - 0 Rv0991c Rv0991c Function unknown Conserved serine rich protein Rv0991c, (MTCI237.05c), len: 110 aa. Conserved ser-rich protein (especially in C-terminus), highly similar to N-terminus of NP_301255.1|NC_002677 conserved hypothetical protein (Ser-rich C-terminus) from Mycobacterium leprae (99 aa). Also highly similar to SCE22.04|AB90971.1|AL355832 hypothetical protein from Streptomyces coelicolor (110 aa); and similar to others. O05574 No conserved hypotheticals O05574 O05574 ML0180,ML0180c MMAR_4523 MSMEG_5479 Mb1018c NC_000962.3 Mycobrowser_v4 CDS 561854 562294 . - 0 Rv0470A Rv0470A Unknown Hypothetical protein Rv0470A, len: 146 aa. Hypothetical unknown protein. GC plot suggests CDS for Cys-rich protein, could possibly be continuation of Rv0471c but no frameshift found to allow this. Sequence same in Mycobacterium bovis and Mycobacterium tuberculosis strain CDC1551. Weak hits to Cys-rich region (aa 258-314) of D63395|D63395_1 mRNA for NOTCH4 from Homo sapiens (1095 aa), FASTA scores: opt: 132, E(): 1.1, (39.35% identity in 61 aa overlap). L7N651 No conserved hypotheticals Q8VKK9 L7N651 GO:0004659,GO:0016021 L7N651 Mb0480c NC_000962.3 Mycobrowser_v4 CDS 562225 562713 . - 0 Rv0471c Rv0471c Unknown Hypothetical protein Rv0471c, (MTV038.15c), len: 162 aa. Hypothetical unknown protein. O53756 No conserved hypotheticals O53756 ML2458 Mb0481c NC_000962.3 Mycobrowser_v4 CDS 563564 564934 . + 0 Rv0473 Rv0473 Unknown Possible conserved transmembrane protein Rv0473, (MTV038.17), len: 456 aa. Possible conserved transmembrane protein, showing some similarity to hypothetical proteins e.g. NP_102800.1|14021975|BAB48586.1|AP002996 hypothetical protein from Mesorhizobium loti (431 aa); P39385|YJIN_ECOLI|YJIN|B4336 hypothetical 48.2 kDa protein (potential integral membrane protein) from Escherichia coli strain K12 (426 aa), FASTA scores: opt: 396, E(): 9.8e-19, (31.8 % identity in 424 aa overlap); etc. Nucleotide position 563577 in the genome sequence has been corrected, A:G resulting in K5R. I6Y3V3 No cell wall and cell processes O53758 I6Y3V3 GO:0016021 I6Y3V3 ML2456,ML2456c MMAR_0798 MSMEG_0917 Mb0483 NC_000962.3 Mycobrowser_v4 CDS 565021 565443 . + 0 Rv0474 Rv0474 Possibly involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv0474, (MTV038.18), len: 140 aa. Probable transcriptional regulator, highly similar to others e.g. CAC04034.1|AL391406 putative DNA-binding protein from Streptomyces coelicolor (141 aa); N-terminus of NP_104173.1|14023352|BAB49959.1|AP003000 transcriptional regulator from Mesorhizobium loti (219 aa); N-terminus of A83618|PA0225 probable transcription regulator from Pseudomonas aeruginosa (179 aa); SINR_BACSU|P06533 sinr protein from Bacillus subtilis (111 aa), FASTA scores: opt: 147, E(): 8.9e-06, (30.6% identity in 111 aa overlap). Also similar to other hypothetical proteins e.g. X66407|RRPHAS_1|ORF1 from Rhodococcus ruber (171 aa), FASTA scores: opt: 280, E(): 4.8e-12, (43.6% identity in 117 aa overlap). Also similar to Rv2745c from Mycobacterium tuberculosis. Contains probable helix-turn-helix domain at aa 35-56 (Score 1709, +5.01 SD). P9WMH9 No regulatory proteins O53759 P9WMH9 GO:0043565,GO:0006350,GO:0045449 P9WMH9 ML2455,ML2455c MMAR_0799 MSMEG_0918 Mb0484 NC_000962.3 Mycobrowser_v4 CDS 594802 595302 . - 0 Rv0504c Rv0504c Function unknown Conserved protein Rv0504c, (MTCY20G9.31c), len: 166 aa. Conserved protein, equivalent to P54879|Y504_MYCLE|ML2425 hypothetical 18.7 KDA protein from Mycobacterium leprae (166 aa), FASTA scores: opt: 884, E(): 0, (83.1% identity in 166 aa overlap); and highly similar to other proteins from Mycobacterium leprae. Also highly similar to CAB77410.1|AL160431|SCD82.07 hypothetical protein from Streptomyces coelicolor (150 aa). Also similar to M. tuberculosis hypothetical proteins Rv0635|H70612 (158 aa); and Rv0637|B70613 (166 aa). P9WFK3 No conserved hypotheticals P64725 P9WFK3 GO:0016491,GO:0008152 P9WFK3 ML2425 Mb0516c NC_000962.3 Mycobrowser_v4 CDS 715370 716317 . + 0 Rv0622 Rv0622 Unknown Possible membrane protein Rv0622, (MTCY20H10.03), len: 315 aa. Possible membrane protein; contains potential membrane spanning region. Shows weak similarity with Mycobacterium tuberculosis hypothetical proteins Rv1804c, Rv1810, etc. Start changed since first submission (-26 aa). P96912 No cell wall and cell processes P96912 P96912 MSMEG_6737 Mb0638 NC_000962.3 Mycobrowser_v4 CDS 567222 567896 . + 0 Rv0478 deoC Involved in nucleotide and deoxyribonucleotide catabolism [catalytic activity: 2-deoxy-D-ribose 5-phosphate = D-glyceraldehyde 3-phosphate + acetaldehyde]. Probable deoxyribose-phosphate aldolase DeoC (phosphodeoxyriboaldolase) (deoxyriboaldolase) Rv0478, (MTCY20G9.04), len: 224 aa. Probable deoC, deoxyribose-phosphate aldolase, equivalent to Q9CB45|DEOC_MYCLE deoxyribose-phosphate aldolase from Mycobacterium leprae (226 aa). Also highly similar to others e.g. DEOC_BACSU|P39121 from Bacillus subtilis (214 aa), FASTA scores: opt: 543, E(): 1.4e-26, (45.9% identity in 209 aa overlap); etc. Belongs to the DEOC/FBAB family of aldolases, DEOC subfamily. P9WP03 No intermediary metabolism and respiration P63929 P9WP03 GO:0005737,GO:0009264,GO:0004139,GO:0016052 4.1.2.4 P9WP03 ML2451,ML2451c MMAR_0803 MSMEG_0922,MSMEG_3089 Mb0488 NC_000962.3 Mycobrowser_v4 CDS 567921 568967 . - 0 Rv0479c Rv0479c Unknown Probable conserved membrane protein Rv0479c, (MTCY20G9.04c), len: 348 aa. Probable conserved membrane protein, equivalent to CAC31967.1|AL583925 possible secreted protein from Mycobacterium leprae (254 aa); and C-terminus highly similar to AAF74996.1|AF143402_1|AF143402 putative multicopper oxidase from Mycobacterium avium (149 aa). Contains hydrophobic domain in centre of protein. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WKV7 No cell wall and cell processes P9WKV7 GO:0005886,GO:0016021 ML2450 MMAR_0804 MSMEG_0923 Mb0489c NC_000962.3 Mycobrowser_v4 CDS 569988 570512 . - 0 Rv0481c Rv0481c Unknown Hypothetical protein Rv0481c, (MTCY20G9.07c), len: 174 aa. Hypothetical unknown protein. P9WKV5 No conserved hypotheticals P64701 P9WKV5 P9WKV5 ML2448 MMAR_0807 MSMEG_0927 Mb0491c NC_000962.3 Mycobrowser_v4 CDS 586394 587380 . + 0 Rv0496 Rv0496 Function unknown Conserved hypothetical protein Rv0496, (MTCY20G9.22), len: 328 aa. Conserved hypothetical protein, highly similar to S72894|467046|AAA17230.1|U00018 exopolyphosphatase ppx from Mycobacterium leprae (406 aa), FASTA scores: opt: 1902, E(): 0, (86.6% identity in 343 aa overlap); and P54882|Y496_MYCLE|ML2434|13094008|CAC31951.1|AL583925 hypothetical 36.2 KDA protein from Mycobacterium leprae (339 aa). Also highly similar to hypothetical proteins and exopolyphosphatases e.g. Q9X8H1|Y715_STRCO|SCE7.15c hypothetical protein from Streptomyces coelicolor (309 aa). C-terminal region similar to CGU31224_1|Q46054 protein similar to ppx gene product of Mycobacterium leprae from Cornybacterium glutamicum (140 aa), FASTA scores: opt: 615, E(): 2.7e-33, (70.9% identity in 134 aa overlap). P9WHV5 No conserved hypotheticals P65786 P9WHV5 GO:0016787 P9WHV5 ML2434,ML2434c MMAR_0822 MSMEG_0939 Mb0507 NC_000962.3 Mycobrowser_v4 CDS 610188 610883 . + 0 Rv0518 Rv0518 Unknown Possible exported protein Rv0518, (MTCY20G10.08), len: 231 aa. Possible exported protein; has hydrophobic N-terminus. O33363 No cell wall and cell processes O33363 GO:0016787 O33363 MMAR_0855 MSMEG_3489 Mb0531 NC_000962.3 Mycobrowser_v4 CDS 1504356 1505078 . + 0 Rv1337 Rv1337 Unknown Probable integral membrane protein Rv1337, (MTCY130.22), len: 240 aa. Probable integral membrane protein. Highly similar to P53426 hypothetical protein B1549_C3_240 from M.leprae (251); and P74553|D90916 hypothetical protein from Synechocystis sp. (198 aa), FASTA scores: E(): 2.3e-25, (43.6% identity in 181 aa overlap). P9WM21 No cell wall and cell processes P9WM21 GO:0005886,GO:0016021 ML1171 MMAR_4059 MSMEG_4904 Mb1372 NC_000962.3 Mycobrowser_v4 CDS 4061899 4062198 . - 0 Rv3622c PE32 Function unknown PE family protein PE32 Rv3622c, (MTCY15C10.30), len: 99 aa. PE32, Member of the Mycobacterium tuberculosis PE family (see Brennan and Delogu, 2002), but no glycine rich C-terminus present. Similar to others e.g. O53938|Rv1788|MTV049.10 (99 aa), FASTA scores: opt: 376, E(): 7.1e-17, (65.6% identity in 96 aa overlap). I6YGS7 No PE/PPE Q7D568 I6YGS7 I6YGS7 MMAR_5122 NC_000962.3 Mycobrowser_v4 ncRNA 575033 575069 . + 0 MTB000060 mcr19 Unknown Fragment of putative small regulatory RNA mcr19, fragment of putative small regulatory RNA (See DiChiara et al., 2010), cloned from M. bovis BCG Pasteur; ends not mapped, 66-82 nt band detected by Northern blot in M. bovis BCG Pasteur. No stable RNAs NC_000962.3 Mycobrowser_v4 CDS 571710 573065 . + 0 Rv0483 lprQ Unknown Probable conserved lipoprotein LprQ Rv0483, (MTCY20G9.09), len: 451 aa. Probable lprQ, conserved lipoprotein, equivalent to CAC31963.1|AL583925|ML2446 possible lipoprotein from Mycobacterium leprae (441 aa); appears longer than ML2446, so start may be further downstream. Shows also similarity with MLCL383_24|O07707 hypothetical 43.6 kDa protein from Mycobacterium leprae; and to Q49706|B1496_F2_81 (271 aa). Similar to others lipoproteins from other organisms. Also similar to several Mycobacterium tuberculosis hypothetical proteins e.g. Rv0116c, Rv0192, Rv1433, Rv2518c. Contains potential N-terminal signal sequence and appropriately positioned PS00013 prokaryotic membrane lipoprotein lipid attachment site. P9WKV3 No cell wall and cell processes P64703 P9WKV3 GO:0016787,GO:0016021,GO:0009252,GO:0008360,GO:0016757,GO:0007047 2.-.-.- P9WKV3 ML2446,ML2446c MMAR_0809 MSMEG_0929 Mb0493 NC_000962.3 Mycobrowser_v4 CDS 573046 573801 . - 0 Rv0484c Rv0484c Function unknown; possibly involved in cellular metabolism. Probable short-chain type oxidoreductase Rv0484c, (MTCY20G9.10c), len: 251 aa. Probable short-chain oxidoreductase, highly similar to others e.g. T36118|4678912|CAB41284.1|AL049707 probable oxidoreductase from Streptomyces coelicolor (260 aa); YDFG_HAEIN|P45200|HI1430 hypothetical oxidoreductase (SDR family) from Haemophilus influenzae (252 aa), FASTA scores: opt: 496, E(): 7.9e-25, (35.0 % identity in 243 aa overlap); etc. Belongs to the short-chain dehydrogenases/reductases (SDR) family. Strong similarity, to bacterial YDFG homologs. P9WGR5 No intermediary metabolism and respiration Q11150 P9WGR5 GO:0055114,GO:0016491 1.-.-.- P9WGR5 ML2445 MMAR_0810 MSMEG_0930 Mb0494c NC_000962.3 Mycobrowser_v4 CDS 573984 575300 . + 0 Rv0485 Rv0485 Possibly involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0485, (MTCY20G9.11), len: 438 aa. Possible transcriptional repressor, member of the NAGC/XYLR repressor family; similar to several e.g. D87820_3|O32446|D82254 NAGC N-acetylglucosamine repressor from Vibrio cholerae (404 aa), FASTA scores: opt: 378, E(): 1.2e-17, (26.9% identity in 350 aa overlap); NAGC_ECOLI|P15301 N-acetylglucosamine repressor from Escherichia coli (406 aa), FASTA scores: opt: 305, E(): 1.8e-12, (21.8% identity in 357 aa overlap); etc. P9WKV1 No regulatory proteins P64705 P9WKV1 P9WKV1 ML2444,ML2444c MMAR_0811 MSMEG_0932 Mb0495 NC_000962.3 Mycobrowser_v4 CDS 585424 586314 . - 0 Rv0495c Rv0495c Function unknown Conserved hypothetical protein Rv0495c, (MTCY20G9.21c), len: 296 aa. Conserved hypothetical protein, highly similar to S72915|B2168_F1_37 hypothetical protein from Mycobacterium leprae (323 aa), FASTA scores: opt: 1615, E(): 0, (82.7% identity in 271 aa overlap); and P54579|Y495_MYCLE|ML243|13094009|CAC31952.1|AL583925 conserved hypothetical protein from Mycobacterium leprae (277 aa). Also highly similar to Q9X8H2|Y716_STRCO|SCE7.16 hypothetical protein from Streptomyces coelicolor (271 aa). P9WKU5 No conserved hypotheticals P9WKU5 ML2435 MMAR_0821 MSMEG_0938 Mb0506c NC_000962.3 Mycobrowser_v4 CDS 587377 588309 . + 0 Rv0497 Rv0497 Unknown Probable conserved transmembrane protein Rv0497, (MTCY20G9.23), len: 310 aa. Probable conserved transmembrane protein, equivalent (but shorter in C-terminus) to P54580|Y497_MYCLE|ML2433 hypothetical 37.9 KDA protein from Mycobacterium leprae (355 aa). N-terminus highly similar to S72922|B2168_C1_166|467074 hypothetical protein from Mycobacterium leprae (118 aa), FASTA scores: opt: 350, E(): 1.4e-12, (57.9% identity in 114 aa overlap); and hydrophobic C-terminus, highly similar to S72895|B2168_C2_209|467047 hypothetical protein from Mycobacterium leprae (241 aa), FASTA scores: opt: 473, E(): 8e-19, (53.9% identity in 241 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WKU3 No cell wall and cell processes P9WKU3 GO:0005886,GO:0016021 ML2433,ML2433c MMAR_0823 MSMEG_0940 Mb0508 NC_000962.3 Mycobrowser_v4 CDS 933003 934031 . - 0 Rv0837c Rv0837c Unknown Hypothetical protein Rv0837c, (MTV043.30c), len: 342 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6Y4Y1 No conserved hypotheticals O53849 I6Y4Y1 I6Y4Y1 Mb0860c NC_000962.3 Mycobrowser_v4 CDS 576787 577338 . + 0 Rv0487 Rv0487 Function unknown Conserved hypothetical protein Rv0487, (MTCY20G9.13), len: 183 aa. Conserved hypothetical protein, highly similar to P54139|Y487_MYCLE|U00018_38|ML2442 hypothetical 20.8 KDA protein from Mycobacterium leprae (184 aa), FASTA scores: opt: 760, E(): 2.4 e-34, (73.0% identity in 159 aa overlap). Also highly similar to CAC04041.1|AL391406 conserved hypothetical protein from Streptomyces coelicolor (168 aa). P9WKU9 No conserved hypotheticals P64709 P9WKU9 P9WKU9 ML2442,ML2442c MMAR_0813 MSMEG_0934 Mb0497 NC_000962.3 Mycobrowser_v4 CDS 578426 579175 . + 0 Rv0489 gpm1 Involved in glycolysis [catalytic activity: 1,3-diphosphoglycerate + 3-phosphoglycerate = 2,3-diphosphoglycerate + 3-phosphoglycerate]. Probable phosphoglycerate mutase 1 Gpm1 (phosphoglyceromutase) (PGAM) (BPG-dependent PGAM) Rv0489, (MTCY20G9.15), len: 249 aa. Probable gpm1, phosphoglycerate mutase 1, equivalent to P53531|PMGY_MYCLE phosphoglycerate mutase from Mycobacterium leprae (247 aa). Also highly similar to others e.g. PMG1_ECOLI|P31217 (249 aa), FASTA scores: opt: 805, E(): 0, (51.4% identity in 245 aa overlap); etc. Contains PS00175 Phosphoglycerate mutase family phosphohistidine signature, and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the phosphoglycerate mutase family. Note that previously known as gpm. P9WIC9 No intermediary metabolism and respiration 1RII P0A5R6 P9WIC9 GO:0006096,GO:0046538 5.4.2.1 P9WIC9 ML2441,ML2441c MMAR_0814 MSMEG_0935 Mb0499 NC_000962.3 Mycobrowser_v4 CDS 588325 589167 . + 0 Rv0498 Rv0498 Function unknown Conserved hypothetical protein Rv0498, (MTCY20G9.24), len: 280 aa. Conserved hypothetical protein, highly similar to P54581|Y498_MYCLE|ML2432 hypothetical 30.5 KDA protein from Mycobacterium leprae (280 aa); and S72896|B2168_C2_210 hypothetical protein from Mycobacterium leprae (244 aa), FASTA scores: opt: 1486, E():0, (89.3% identity in 244 aa overlap). Also similar to Q9X8H0|Y714_STRCO|SCE7.14c hypothetical protein from Streptomyces coelicolor. P9WKU1 No conserved hypotheticals P64717 P9WKU1 P9WKU1 ML2432,ML2432c MMAR_0824 MSMEG_0941 Mb0509 NC_000962.3 Mycobrowser_v4 CDS 1485313 1485771 . - 0 Rv1322A Rv1322A Function unknown Conserved protein Rv1322A, len: 152 aa. Conserved protein, similar to proteins from Mycobacterium leprae and Streptomyces coelicolor e.g. AL583921_2|ML1157 from M. leprae strain tn (155 aa), FASTA scores: opt: 771, E(): 5.1e-43, (75.3% identity in 154 aa overlap); and AL137242_2 from Streptomyces coelicolor (146 aa), FASTA scores: opt: 404, E(): 2e-19, (43.165% identity in 139 aa overlap). L7N6B1 No conserved hypotheticals Q8VK36 L7N6B1 GO:0016702 L7N6B1 ML1157,ML1157c MMAR_4076 MSMEG_4921 Mb1357c NC_000962.3 Mycobrowser_v4 CDS 1791334 1791573 . + 0 Rv1590 Rv1590 Function unknown Conserved hypothetical protein Rv1590, (MTCY336.14c), len: 79 aa. Conserved hypothetical protein, similar to Q49616|LEPB1170_C1_162|YF90_MYCLE from Mycobacterium leprae (80 aa), FASTA scores: opt: 368, E(): 1.7e-21, Smith-Waterman score: 368, (67.1% identity in 73 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WLT7 No conserved hypotheticals P9WLT7 ML1221 MMAR_5574 MSMEG_3195 Mb1616 NC_000962.3 Mycobrowser_v4 CDS 2261098 2261688 . + 0 Rv2014 Rv2014 Required for the transposition of an insertion element. Transposase Rv2014, (MTCY39.03c), len: 196 aa. Transposase, similar to insertion elements; possibly made by frameshifting with respect to Rv2013. Length changed since first submission. Q10843 No insertion seqs and phages Q10843 Q10843 GO:0004803,GO:0006313,GO:0003677 Mb2037 NC_000962.3 Mycobrowser_v4 CDS 2544698 2545225 . + 0 Rv2270 lppN Unknown Probable lipoprotein LppN Rv2270, (MTCY339.40c), len: 175 aa. Probable lppN, lipoprotein; has appropriately positioned prokaryotic membrane lipoprotein attachment site PS00013. P9WK73 No cell wall and cell processes P9WK73 GO:0005886 MMAR_3366 Mb2293 NC_000962.3 Mycobrowser_v4 CDS 583701 584690 . - 0 Rv0493c Rv0493c Function unknown Conserved protein Rv0493c, (MTCY20G9.19), len: 329 aa. Conserved protein, showing some similarity to U00018_33|B2168_F2_93 from Mycobacterium leprae (167 aa), FASTA scores: opt: 166, E(): 0.00077, (35.9% identity in 131 aa overlap). P9WKU7 No conserved hypotheticals P9WKU7 ML2437 MMAR_0819 Mb0504c NC_000962.3 Mycobrowser_v4 CDS 584695 585423 . + 0 Rv0494 Rv0494 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably GntR-family) Rv0494, (MTCY20G9.20), len: 242 aa. Probable transcriptional regulator, GntR family, with C-terminal part highly similar to S72893|B2168_C2_205 hypothetical protein from Mycobacterium leprae (105 aa). Also similar to other transcription regulators e.g. PDHR_ECOLI|P06957 pyruvate dehydrogenase complex repressor PDHR or GENA from Escherichia coli (254 aa), FASTA scores: opt: 284, E(): 1.2e-11, (32.6% identity in 224 aa overlap); etc. Contains PS00043 Bacterial regulatory proteins, gntR family signature, and probable helix-turn helix motif from aa 50-71 (Score 1229, +3.37 SD). P9WMG7 No regulatory proteins P67739 P9WMG7 GO:0006355,GO:0006350,GO:0003700,GO:0005622 P9WMG7 ML2436,ML2436c MMAR_0820 MSMEG_2173,MSMEG_2794 Mb0505 NC_000962.3 Mycobrowser_v4 CDS 606551 608062 . + 0 Rv0515 Rv0515 Function unknown Conserved 13E12 repeat family protein Rv0515, (MTCY20G10.05), len: 503 aa. Part of M. tuberculosis 13E12 repeat family. Almost identical to Rv0336 (99.8% identity in 503 aa overlap), possibly due to a recent gene duplication. Also similar to other M. tuberculosis hypothetical 13E12 repeat proteins e.g. Rv1148c, Rv1945, etc. O33360 No insertion seqs and phages O33360 O33360 GO:0003676,GO:0004519 Mb0528 NC_000962.3 Mycobrowser_v4 CDS 592791 593867 . + 0 Rv0502 Rv0502 Function unknown Conserved protein Rv0502, (MTCY20G9.29), len: 358 aa. Conserved protein, equivalent to P54878|Y502_MYCLE|ML2427 hypothetical 40.5 KDA protein from Mycobacterium leprae (367 aa), FASTA scores: opt: 2042, E(): 0, (84.1% identity in 365 aa overlap). Also similar to T36273|SCE68.23c hypothetical protein from Streptomyces coelicolor (355 aa). C-terminal similar to AL021529|SC10A5_4|T34572 hypothetical protein from Streptomyces coelicolor (295 aa), FASTA score: (57.8% identity in 263 aa overlap); and to hypothetical proteins from Mycobacterium tuberculosis Rv1920|G70808 (287 aa); and Rv1428c|G70914 (275 aa). P9WKT1 No conserved hypotheticals P64723 P9WKT1 GO:0008152,GO:0008415 P9WKT1 ML2427,ML2427c MMAR_0830 MSMEG_0947 Mb0514 NC_000962.3 Mycobrowser_v4 CDS 1733116 1733550 . - 0 Rv1532c Rv1532c Function unknown Conserved hypothetical protein Rv1532c, (MTCY07A7A.01c), len: 144 aa. Conserved hypothetical protein, similar to P20378|YPHR_HALHA Hypothetical 15.6 kDa protein from Halobacterium halobium (151 aa), FASTA scores: opt: 152, E():4.5e-05, (30.1% identity in 103 aa overlap). O06178 No conserved hypotheticals O06178 O06178 O06178 MMAR_4134 MSMEG_4984 Mb1559c NC_000962.3 Mycobrowser_v4 CDS 4116002 4116379 . + 0 Rv3675 Rv3675 Unknown Possible membrane protein Rv3675, (MTV025.023), len: 125 aa. Possible membrane protein, with some similarity to Q9YCZ2|APE1120 hypothetical 11.7 KDA protein from Aeropyrum pernix (103 aa), FASTA scores: opt: 100, E(): 9, (40.0% identity in 55 aa overlap). I6YCQ7 No cell wall and cell processes I6YCQ7 I6YCQ7 Mb3699 NC_000962.3 Mycobrowser_v4 CDS 590083 590970 . + 0 Rv0500 proC Involved at the terminal (third) step in proline biosynthesis [catalytic activity: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H]. Probable pyrroline-5-carboxylate reductase ProC (P5CR) (P5C reductase) Rv0500, (MTCY20G9.26), len: 295 aa. Probable proC, Pyrroline-5-carboxylate reductase (see citation below), equivalent to P46725|PROC_MYCLE pyrroline-5-carboxylate reductase from Mycobacterium leprae (294 aa), FASTA scores: opt: 1473, E(): 0, (82.4% identity in 295 aa overlap). Also similar to others e.g. P46540|PROC_CORGL pyrroline-5-carboxylate reductase from Corynebacterium glutamicum (270 aa); T36286|4803683|CAB42663.1|AL049819 pyrroline-5-carboxylate reductase from Streptomyces coelicolor (284 aa); etc. Belongs to the pyrroline-5-carboxylate reductase family. P9WHU7 No intermediary metabolism and respiration Q11141 P9WHU7 GO:0005737,GO:0055114,GO:0006561,GO:0004735 1.5.1.2 P9WHU7 ML2430,ML2430c MMAR_0826 MSMEG_0943 Mb0511 NC_000962.3 Mycobrowser_v4 CDS 597199 600105 . + 0 Rv0507 mmpL2 Unknown. Thought to be involved in fatty acid transport. Probable conserved transmembrane transport protein MmpL2 Rv0507, (MTCY20G9.34), len: 968 aa. Probable mmpL2, conserved transmembrane transport protein (see citations below), member of RND superfamily, highly similar to other Mycobacterial proteins e.g. YV34_MYCLE from Mycobacterium leprae (959 aa), FASTA scores: opt: 3699, E(): 0, (58.3% identity in 940 aa overlap); and the Mycobacterium tuberculosis proteins MTV037_14, MTV040_4, MTCY98_8, MTCY4D9_15, MTCY48_8, MTCY19G5_6, MTV005_19, etc. Also similar to STMACTII_3|SC10A5_9 from Streptomyces coelicolor; and BSUB0|004_12 from Bacillus subtilis. C-terminal half similar to Q50086|U1740AB from Mycobacterium leprae (386 aa), FASTA scores: opt: 1526, E(): 0, (61.5% identity in 371 aa overlap). Belongs to the MmpL family. P9WJV7 No cell wall and cell processes Q11171 P9WJV7 GO:0005886,GO:0016021 MMAR_0840 Mb0519 NC_000962.3 Mycobrowser_v4 CDS 600441 601847 . + 0 Rv0509 hemA Involved in porphyrin biosynthesis by the C5 pathway (at the first step) [catalytic activity: glutamyl-tRNA(GLU) + NADPH = glutamate-1-semialdehyde + NADP+ + tRNA(GLU)]. Probable glutamyl-tRNA reductase HemA (GLUTR) Rv0509, (MTCY20G9.36), len: 468 aa. Probable hemA, glutamyl-tRNA reductase, equivalent to HEM1_MYCLE|P46724 glutamyl-tRNA reductase from Mycobacterium leprae (467 aa), FASTA scores: opt: 2377, E(): 0, (82.3% identity in 463 aa overlap). Also highly similar (sometimes in part) to others e.g. Q9WX15|HEM1_STRCO glutamyl-tRNA reductase from Streptomyces coelicolor (581 aa); P16618|HEM1_BACSU|HEMA glutamyl-tRNA reductase from Bacillus subtilis (455 aa); etc. Contains PS00747 Glutamyl-tRNA reductase signature. Belongs to the glutamyl-tRNA reductase family. P9WMP7 No intermediary metabolism and respiration P64328 P9WMP7 GO:0005737,GO:0008883,GO:0055114,GO:0006779,GO:0004764,GO:0050661 1.2.1.70 P9WMP7 ML2422,ML2422c MMAR_0842 MSMEG_0952 Mb0521,Mb0522 NC_000962.3 Mycobrowser_v4 CDS 611172 612074 . - 0 Rv0519c Rv0519c Unknown; could have possibly a lipolytic activity. Possible conserved membrane protein Rv0519c, (MTCY20G10.09c), len: 300 aa. Possible conserved membrane protein, with hydrophobic region near N-terminus. Could be a lipase. Similar to Rv0774c|MTCY369.19c|A70708 from Mycobacterium tuberculosis (312 aa), FASTA scores: opt: 1092, E(): 0, (57.9% identity in 299 aa overlap). Contains PS00120 Lipases, serine active site. O33364 No cell wall and cell processes O33364 O33364 O33364 ML2417 MMAR_0858 Mb0532c NC_000962.3 Mycobrowser_v4 tRNA 4126541 4126614 . + 0 MTB000045 proY - tRNA-Pro (CGG) proY, tRNA-Pro, anticodon cgg, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v4 CDS 604602 605591 . + 0 Rv0512 hemB Involved in porphyrin and heme biosynthesis (at the second step) [catalytic activity: 2 5-aminolevulinate = porphobilinogen + 2 H2O]. Probable delta-aminolevulinic acid dehydratase HemB (porphobilinogen synthase) (ALAD) (ALADH) Rv0512, (MTCY20G10.02), len: 329 aa. Probable hemB, delta-aminolevulinic acid dehydratase, equivalent to 46723|HEM2_MYCLE delta-aminolevulinic acid dehydratase from Mycobacterium leprae (329 aa). Also highly similar to many e.g. P54919|HEM2_STRCO from Streptomyces coelicolor (330 aa); HEM2_ECOLI|P15002 from Escherichia coli (323 aa), FASTA scores: opt: 942, E(): 0, (47.6% identity in 317 aa overlap); etc. Contains PS00169 Delta-aminolevulinic acid dehydratase active site. Belongs to the ALADH family. Cofactor: zinc. P9WMP5 No intermediary metabolism and respiration O33357 P9WMP5 GO:0046872,GO:0004655,GO:0006783 4.2.1.24 P9WMP5 ML2419,ML2419c MMAR_0845 MSMEG_0956 Mb0525 NC_000962.3 Mycobrowser_v4 CDS 605604 606152 . + 0 Rv0513 Rv0513 Unknown Possible conserved transmembrane protein Rv0513, (MTCY20G10.03), len: 182 aa. Possible conserved transmembrane protein, with its N-terminus highly similar to S72925|B2168_C1_182 hypothetical protein from Mycobacterium leprae (103 aa), FASTA scores: opt: 217, E(): 8.2e-14, (45.3 % identity in 106 aa overlap). O33358 No cell wall and cell processes O33358 GO:0016021 ML2418,ML2418c MMAR_0846 MSMEG_0959 Mb0526 NC_000962.3 Mycobrowser_v4 CDS 608746 610056 . + 0 Rv0517 Rv0517 Function unknown; probably involved in cellular metabolism. Possible membrane acyltransferase Rv0517, (MTCY20G10.07), len: 436 aa. Possible acyltransferase, integral membrane protein, equivalent (but longer 26 aa in N-terminus) to AAK44761.1|AE006954 putative acyltransferase from Mycobacterium tuberculosis strain CDC1551 (410 aa). Also similar to many acyltransferases e.g. MDMB_STRMY|Q00718 from Streptomyces mycarofaciens (387 aa), FASTA scores: opt: 200, E(): 1.1e-08, (28.2% identity in 394 aa overlap). And similar to Rv0111, Rv0228, Rv1254, Rv1565c from Mycobacterium tuberculosis. O33362 No intermediary metabolism and respiration O33362 O33362 GO:0016020,GO:0008415 2.3.1.- MMAR_0854 MSMEG_3490 Mb0530 NC_000962.3 Mycobrowser_v4 CDS 1530173 1530925 . + 0 Rv1359 Rv1359 Involved in transcriptional mechanism Probable transcriptional regulatory protein Rv1359, (MTCY02B10.23), len: 250 aa. Probable transcriptional regulatory protein, similar to Rv0891c|MTCY31.19c, (48.5% identity in 204 aa overlap) and to Rv1358|MTCY02B10.22 (43.7% identity in 197 aa overlap). P9WM05 No regulatory proteins P9WM05 GO:0016849,GO:0009190 P9WM05 Mb1394 NC_000962.3 Mycobrowser_v4 CDS 1640680 1641543 . + 0 Rv1455 Rv1455 Function unknown Conserved protein Rv1455, (MTV007.02), len: 287 aa. Conserved protein, some similarity from aa 80-160 to Z99125|MLCL536.35c hypothetical Mycobacterium leprae protein (101 aa), FASTA scores: opt: 238, E(): 1.8e-08, (51.3% identity in 78 aa overlap). O53147 No conserved hypotheticals O53147 O53147 ML0586 MMAR_2260 MSMEG_3107 Mb1490 NC_000962.3 Mycobrowser_v4 CDS 614326 614721 . - 0 Rv0523c Rv0523c Function unknown Conserved protein Rv0523c, (MTCY25D10.02), len: 131 aa. Conserved protein, showing some similarity to M. tuberculosis proteins Rv1598c|MTCY336.06; and Rv1871c|MTCY336_06|O06592 (136 aa), FASTA scores: opt: 197, E(): 5e-08, (38.4% identity in 99 aa overlap). O06389 No conserved hypotheticals O06389 O06389 ML2415 Mb0536c NC_000962.3 Mycobrowser_v4 CDS 616223 616831 . + 0 Rv0525 Rv0525 Function unknown Conserved protein Rv0525, (MTCY25D10.04), len: 202 aa. Conserved protein, equivalent to Q49821|B2168_C3_276|S72912 hypothetical protein from Mycobacterium leprae (202 aa), FASTA scores: opt: 1151, E(): 0, (82.5% identity in 200 aa overlap). Also highly similar to CAC08377.1|AL392176 putative phosphoglycerate mutase from Streptomyces coelicolor (233 aa); and similar to SLL0395|Q55734 hypothetical 23.8 kDa protein from synechocystis SP. (212 aa), FASTA scores: opt: 207, E(): 5.1e-07, (28.2% identity in 195 aa overlap). Also some similarity to Rv2228c|Y019_MYCTU|Q10512|cy427.09 hypothetical 39.2 kDa protein from Mycobacterium tuberculosis (364 aa), FASTA scores: opt: 236, E(): 1.1e-08, (34.3% identity in 198 aa overlap). O06391 No conserved hypotheticals O06391 O06391 O06391 ML2413,ML2413c MMAR_0871 MSMEG_0970 Mb0538 NC_000962.3 Mycobrowser_v4 CDS 616846 617496 . + 0 Rv0526 Rv0526 Possibly acts on thioredoxin Possible thioredoxin protein (thiol-disulfide interchange protein) Rv0526, (MTCY25D10.05), len: 216 aa. Possible thioredoxin protein (thiol-disulfide interchange protein) , equivalent to Q49816|U2168C|S72901 hypothetical protein from Mycobacterium leprae (216 aa), FASTA scores: opt: 1144, E(): 0, (78.5% identity in 214 aa overlap). C-terminus shows some similarity to C-terminus of thioredoxins e.g. RESA_BACSU|P35160 resa protein from Bacillus subtilis (181 aa), FASTA scores: opt: 200, E(): 7.4e-06, (24.2% identity in 132 aa overlap); etc. Also similar to Mycobacterium tuberculosis thioredoxin-like proteins Rv1470, Rv1471, Rv1677, etc. Contains PS00194 Thioredoxin family active site. Seems to belong to the thioredoxin family. O06392 No intermediary metabolism and respiration O06392 O06392 GO:0045454,GO:0055114,GO:0016491,GO:0016209 1.-.-.- O06392 ML2412,ML2412c MMAR_0872 MSMEG_0971 Mb0539 NC_000962.3 Mycobrowser_v4 CDS 622793 624577 . + 0 Rv0532 PE_PGRS6 Function unknown PE-PGRS family protein PE_PGRS6 Rv0532, (MTCY25D10.11), len: 594 aa. PE_PGRS6, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), similar to others e.g. Y0DP_MYCTU|Q50615 from Mycobacterium tuberculosis (498 aa), FASTA scores: opt: 1703, E(): 0, (58.2% identity in 536 aa overlap). L0T3X8 No PE/PPE Q6MX30 L0T3X8 L0T3X8 MMAR_2097,MMAR_2596,MMAR_4149,MMAR_4561,MMAR_5339 Mb0545,Mb0546 NC_000962.3 Mycobrowser_v4 CDS 1743778 1744371 . - 0 Rv1541c lprI Unknown Possible lipoprotein LprI Rv1541c, (MTCY48.24), len: 197 aa. Possible lipoprotein lprI, contains appropriately positioned prokaryotic membrane lipoprotein lipid attachment site (PS0013). P9WK41 No cell wall and cell processes P65318 P9WK41 GO:0005886 P9WK41 ML1202,ML1202c MMAR_2364 Mb1568c NC_000962.3 Mycobrowser_v4 CDS 618305 619894 . + 0 Rv0528 Rv0528 Unknown Probable conserved transmembrane protein Rv0528, (MTCY25D10.07), len: 529 aa. Probable conserved transmembrane protein, equivalent (shorter 14 aa in N-terminus) to CAC31926.1|AL583925 conserved membrane protein from Mycobacterium leprae (542 aa). Also highly similar to Q49817|B2168_C2_237|S72902 hypothetical protein from Mycobacterium leprae (364 aa), FASTA scores: opt: 1846, E(): 0, (81.1% identity in 338 aa overlap); and Q49811|B2168_C1_194|S72891 hypothetical protein from Mycobacterium leprae (106 aa), FASTA scores: opt: 506, E(): 3.8e-26, (73.6% identity in 106 aa overlap). Also highly similar to CAC08381.1|AL392176 putative integral membrane protein from Streptomyces coelicolor (574 aa). O06394 No cell wall and cell processes O06394 O06394 GO:0016021 ML2410,ML2410c MMAR_0874 MSMEG_0973 Mb0541 NC_000962.3 Mycobrowser_v4 CDS 619891 620865 . + 0 Rv0529 ccsA Required during cytochrome biogenesis at the step of heme attachment. Possible cytochrome C-type biogenesis protein CcsA Rv0529, (MTCY25D10.08), len: 324 aa. Possible ccsA, cytochrome C-type biogenesis protein, integral membrane protein, equivalent to NP_302558.1|NC_002677|B2168_C3_281 possible cytochrome C biogenesis protein from Mycobacterium leprae (327 aa), FASTA scores: opt: 1779, E(): 0, (82.9% identity in 327 aa overlap). Also highly similar to others e.g. CAC08382.1|AL392176 putative cytochrome biogenesis related protein from Streptomyces coelicolor (380 aa); CCSA_CHLRE|P48269 probable cytochrome c biogenesis protein from Chlamydomonas reinhardtii (353 aa), FASTA scores: opt: 449, E(): 1.3e-23, (34.4% identity in 247 aa overlap); etc. Belongs to the CCMF/CYCK/CCL1/NRFE/CCSA family. Note that previously known as ccsB. O06393 No intermediary metabolism and respiration O06393 O06393 GO:0008535,GO:0016020 O06393 ML2409,ML2409c MMAR_0875 MSMEG_0974 Mb0542 NC_000962.3 Mycobrowser_v4 CDS 620907 622124 . + 0 Rv0530 Rv0530 Function unknown Conserved protein Rv0530, (MTCY25D10.09), len: 405 aa. Conserved protein, similar in part to other hypothetical proteins e.g. AL031231|SC3C3_3|CAA20252.1 from Streptomyces coelicolor (1083 aa), FASTA scores: opt: 870, E(): 0, (39.5% identity in 443 aa overlap); etc. Also similar to Mycobacterium tuberculosis proteins e.g. Rv3868, Rv0282, Rv1798, etc. O06396 No conserved hypotheticals O06396 O06396 O06396 ML2408,ML2408c MMAR_0876 MSMEG_0975 Mb0543 NC_000962.3 Mycobrowser_v4 CDS 622121 622282 . - 0 Rv0530A Rv0530A Unknown Conserved protein Rv0530A, len: 53 aa. Conserved protein. V5QPR5 No conserved hypotheticals V5QPR5 NC_000962.3 Mycobrowser_v4 CDS 650779 651504 . - 0 Rv0560c Rv0560c Possibly causes methylation Possible benzoquinone methyltransferase (methylase) Rv0560c, (MTCY25D10.39c), len: 241 aa. Possible benzoquinone methyltransferase (see citation below), similar to other hypothetical proteins and methyltransferases e.g. Q54300 methyltransferase (211 aa), FASTA scores: opt: 203, E(): 4.8e-07, (30.9% identity in 136 aa overlap). Similar to Rv3699, Rv1377c, Rv2675c, etc from Mycobacterium tuberculosis. Rv0560c can be induced by salicylate and para-amino-salicylate (pas). P9WKL5 No intermediary metabolism and respiration O06426 P9WKL5 GO:0008168,GO:0008152 2.1.1.- P9WKL5 ML2275,ML2275c MMAR_0909 MSMEG_2539 Mb0575c NC_000962.3 Mycobrowser_v4 CDS 779543 780040 . - 0 Rv0679c Rv0679c Function unknown Conserved threonine rich protein Rv0679c, (MTV040.07c), len: 165 aa. Conserved Thr-rich protein, similar in part to neighboring ORF Rv0680c (124 aa), FASTA score: (35.1% identity in 131 aa overlap); and Rv0314c (220 aa). Predicted to be an outer membrane protein (See Song et al., 2008). I6WZ30 No conserved hypotheticals O53787 I6WZ30 ML1884 MMAR_1008 MSMEG_1393 Mb0698c NC_000962.3 Mycobrowser_v4 CDS 842033 842278 . + 0 Rv0750 Rv0750 Function unknown Conserved hypothetical protein Rv0750, (MTV041.24), len: 81 aa. Conserved hypothetical protein, showing almost perfect overlap with C-terminus of Rv0740|MTV041_14 conserved hypothetical protein from Mycobacterium tuberculosis (175 aa), FASTA scores: (93.8% identity in 81 aa overlap). Possible duplication. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53813 No conserved hypotheticals O53813 Mb0772 NC_000962.3 Mycobrowser_v4 CDS 1241971 1242831 . - 0 Rv1118c Rv1118c Function unknown Conserved protein Rv1118c, (MTCY22G8.07c), len: 286 aa. Conserved protein, similar to pseudogene ML0942 in Mycobacterium leprae. O06570 No conserved hypotheticals O06570 O06570 ML0942,ML0942c MMAR_4342 MSMEG_5213 Mb1149c NC_000962.3 Mycobrowser_v4 CDS 1242864 1243013 . - 0 Rv1119c Rv1119c Unknown Hypothetical protein Rv1119c, (MTCY22G8.08c), len: 49 aa. Hypothetical unknown protein. O06571 No conserved hypotheticals O06571 O06571 Mb1150c NC_000962.3 Mycobrowser_v4 CDS 625562 626440 . - 0 Rv0534c menA Involved in menaquinone biosynthesis. Conversion of 1,4-dihydroxy-2-naphthoate (DHNA) to dimethylmenaquinone (DMK). Attaches octaprenylpyrophosphate, a membrane-bound 40-carbon side chain to DHNA. The conversion of DHNA to DMK proceeds in three stages: the removal of the carboxyl group of DHNA as CO2, the attachment of the isoprenoid side chain, and a quinol-to-quinone oxidation, which is thought to be spontaneous. 1,4-dihydroxy-2-naphthoate octaprenyltransferase MenA (DHNA-octaprenyltransferase) Rv0534c, (MTCY25D10.13c), len: 292 aa. Probable menA, 1,4-dihydroxy-2-naphthoate octaprenyltransferase, integral membrane protein, equivalent to Y13803|MLB1306_2|NP_302556.1 probable 4-dihydroxy-2-naphthoate octaprenyltransferase from Mycobacterium leprae (294 aa), FASTA scores: opt: 1509, E(): 0, (80.2% identity in 288 aa overlap). Also highly similar to others e.g. MENA_ECOLI|P32166|B3930 from Escherichia coli (308 aa), FASTA scores: opt: 495, E(): 2.9e-25, (36.3 identity in 289 aa overlap); etc. Belongs to the MenA family. P9WIP3 No intermediary metabolism and respiration P65650 P9WIP3 GO:0009234,GO:0005886,GO:0004659,GO:0016021 2.5.1.74 ML2406 MMAR_0880 MSMEG_0988 Mb0548c NC_000962.3 Mycobrowser_v4 CDS 633055 634404 . - 0 Rv0541c Rv0541c Unknown Probable conserved integral membrane protein Rv0541c, (MTCY25D10.20c), len: 449 aa. Probable conserved integral membrane protein, highly similar (except first 40 residues) to CAB76994.1|AL159178 putative integral membrane protein from Streptomyces coelicolor (456 aa). Also some similarity to Q13724|GCS1_HUMAN mannosyl-oligosaccharide glucosidase (834 aa), FASTA scores: opt: 150, E(): 0.013, (27.1% identity in 339 aa overlap). Contains PS00041 Bacterial regulatory proteins, araC family signature. O06407 No cell wall and cell processes O06407 O06407 ML2256,ML2256c MMAR_0887 MSMEG_0999 Mb0555c NC_000962.3 Mycobrowser_v4 CDS 697154 697567 . - 0 Rv0598c vapC27 Unknown Possible toxin VapC27. Contains PIN domain. Rv0598c, (MTCY19H5.24), len: 137 aa. Possible vapC27, toxin, part of toxin-antitoxin (TA) operon with Rv00599c, contains PIN domain, see Arcus et al. 2005. Similar to others e.g. Rv2596|Y0B5_MYCTU|Q50625 conserved hypothetical protein from Mycobacterium tuberculosis (134 aa), FASTA scores: opt: 254, E(): 8.2e-12, (41.5% identity in 130 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WF83 No virulence, detoxification, adaptation O07780 P9WF83 P9WF83 Mb0614c NC_000962.3 Mycobrowser_v4 CDS 1264606 1264947 . + 0 Rv1136 Rv1136 Involved in carnitine metabolism. Possible enoyl-CoA hydratase Rv1136, (MTCI65.03), len: 113 aa. Probable enoyl-CoA hydratase (possible gene fragment). Some similarity to N-terminus of carnitine racemases and enoyl-CoA hydratases (but much shorter) e.g. I41014 carnitine racemase from Escherichia coli (297 aa), FASTA scores: opt: 258, E(): 2.5e-11, (44.5% identity in 110 aa overlap); and Rv0222 putative enoyl-CoA hydratase from M. tuberculosis (262 aa). O06536 No lipid metabolism O06536 O06536 GO:0008152,GO:0016853 5.-.-.- O06536 Mb1168 NC_000962.3 Mycobrowser_v4 CDS 628298 629731 . - 0 Rv0537c Rv0537c Unknown Probable integral membrane protein Rv0537c, (MTCY25D10.16c), len: 477 aa. Probable integral membrane protein, showing weak similarity to YDNK_STRCO|P40180 hypothetical 41.2 kDa protein from Streptomyces coelicolor (411 aa), FASTA scores: opt: 122, E(): 0.85, (28.2% identity in 373 aa overlap). O06403 No cell wall and cell processes O06403 O06403 ML0305,ML0305c MMAR_0883 MSMEG_1189 Mb0551c NC_000962.3 Mycobrowser_v4 CDS 631743 632375 . + 0 Rv0539 Rv0539 Substrate (sugar) unknown [catalytic activity: NDP-sugar + dolichyl phosphate = NDP + dolichyl sugar phosphate]. Probable dolichyl-phosphate sugar synthase (dolichol-phosphate sugar synthetase) (dolichol-phosphate sugar transferase) (sugar phosphoryldolichol synthase) Rv0539, (MTCY25D10.18), len: 210 aa. Probable dolichol-P-sugar synthase, highly similar to CAB76989.1|AL159178 putative glycosyltransferase from Streptomyces coelicolor (242 aa), and similar to various dolichol-P-sugar synthetases and sugar transferases e.g. NP_126257.1|NC_000868 dolichyl-phosphate mannose synthase related protein from Pyrococcus abyssi (211 aa); N-terminus of NP_127133.1|NC_000868 dolichol-P-glucose synthetase from Pyrococcus abyssi (378 aa); N-terminus of NP_068880.1|NC_000917 putative dolichol-P-glucose synthetase from Archaeoglobus fulgidus (369 aa), FASTA scores: E(): 2.4e-13, (32. 1% identity in 193 aa overlap); Q26732 dolichyl-phosphate-mannose synthase precursor from trypanosoma brucei (267 aa), FASTA scores: opt: 179, E(): 0.0011, (30.7% identity in 205 aa overlap); etc. Also similar to Rv2051c|MTY25D10_18 from Mycobacterium tuberculosis. Contains PS00017 ATP/GTP-binding site motif A (P-loop). P9WMY1 No intermediary metabolism and respiration P0A597 P9WMY1 GO:0016757 2.4.-.- P9WMY1 MMAR_0885 MSMEG_0995 Mb0553 NC_000962.3 Mycobrowser_v4 CDS 635573 635875 . - 0 Rv0543c Rv0543c Function unknown Conserved protein Rv0543c, (MTCY25D10.22c), len: 100 aa. Conserved protein, equivalent to Q50171|MLU15187_32|NP_302469.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (100 aa), FASTA scores: opt: 493, E(): 6.1e-30, (73.5% identity in 98 aa overlap). Some similarity to Rv3046c|NP_217562.1 from Mycobacterium tuberculosis. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Alternative nucleotide at position 635633 (C->T; A81A) has been observed. O06409 No conserved hypotheticals O06409 O06409 O06409 ML2258,ML2258c MMAR_0889 MSMEG_1063 Mb0557c NC_000962.3 Mycobrowser_v4 CDS 637583 637969 . - 0 Rv0546c Rv0546c Function unknown Conserved protein Rv0546c, (MTCY25D10.25c), len: 128 aa. Conserved protein, equivalent to AAA63111.1|U15187|Q50174|U296X hypothetical protein from Mycobacterium leprae (144 aa), FASTA scores: opt: 748, E(): 0, (84.2% identity in 133 aa overlap). Also highly similar to CAB95979.1|AL360034 conserved hypothetical protein from Streptomyces coelicolor (130 aa); and similar to AE000854_8|O26852 S-D-lactoylglutathione methylglyoxal lyase from Methanobacterium thermoautotropto (116 aa), FASTA scores: opt: 155, E(): 0.00019, (30.6% identity in 108 aa overlap); YAER_ECOLI hypothetical 14.7 kDa protein from Escherichia coli (129 aa), FASTA scores: opt: 104, E(): 0.42, (28.7% identity in 115 aa overlap). Also similar to Rv2068c from Mycobacterium tuberculosis. O06412 No conserved hypotheticals O06412 O06412 O06412 ML2261,ML2261c MMAR_0892 MSMEG_1067 Mb0560c NC_000962.3 Mycobrowser_v4 CDS 638032 638916 . - 0 Rv0547c Rv0547c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0547c, (MTCY25D10.26c), len: 294 aa. Possible oxidoreductase, similar to various oxidoreductases e.g. fatty acyl-CoA reductase from Acinetobacter calcoaceticus (295 aa); NP_280196.1|NC_002607 3-oxoacyl-[acyl-carrier-protein] reductase from Halobacterium sp. NRC-1 (255 aa); NP_349214.1|NC_003030 Short-chain alcohol dehydrogenase family protein from Clostridium acetobutylicum (255 aa); etc. Also similar to several proteins from Mycobacterium tuberculosis e.g. Y04M_MYCTU|Q10783 putative oxidoreductase (341 aa), FASTA scores: opt: 644, E(): 0, (46.1% identity in 258 aa overlap). O06413 No intermediary metabolism and respiration O06413 O06413 GO:0055114,GO:0016491 1.-.-.- O06413 ML2262,ML2262c MMAR_0894 MSMEG_1073 Mb0561c NC_000962.3 Mycobrowser_v4 CDS 639012 639956 . - 0 Rv0548c menB Involved in menaquinone biosynthesis. Convert O-succinylbenzoyl-CoA (OSB-CoA) to 1,4-dihydroxy-2-naphthoic acid (DHNA) [catalytic activity: O-succinylbenzoyl-CoA = 1,4-dihydroxy-2-naphthoate + CoA]. Naphthoate synthase MenB (dihydroxynaphthoic acid synthetase) (DHNA synthetase) Rv0548c, (MTCY25D10.27c), len: 314 aa. menB, naphthoate synthase (dihydroxynaphthonic acid synthase), equivalent to NP_302473.1|NC_002677 naphthoate synthase from Mycobacterium leprae (300 aa). Also similar to others e.g. MENB_ECOLI|P27290 naphthoate synthase from Escherichia coli (285 aa), FASTA scores: opt: 599, E(): 9.3e-33, (48.1 identity in 285 aa overlap); etc. Belongs to the enoyl-CoA hydratase/isomerase family. P9WNP5 No intermediary metabolism and respiration 1Q51,1Q52,1RJM,1RJN,3T8A,3T8B O06414 P9WNP5 GO:0008935,GO:0009234 4.1.3.36 P9WNP5 ML2263,ML2263c MMAR_0895 MSMEG_1075 Mb0562c NC_000962.3 Mycobrowser_v4 CDS 640228 640641 . - 0 Rv0549c vapC3 Unknown Possible toxin VapC3 Rv0549c, (MTCY25D10.28c), len: 137 aa. Possible vapC3, toxin, part of toxin-antitoxin (TA) operon with Rv0550c, contains PIN domain (see Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others e.g. Rv0960, Rv0065, and Rv1720c from Mycobacterium tuberculosis. P9WFB7 No virulence, detoxification, adaptation O06415 P9WFB7 P9WFB7 Mb0563c,Mb0564c NC_000962.3 Mycobrowser_v4 CDS 640638 640904 . - 0 Rv0550c vapB3 Unknown Possible antitoxin VapB3 Rv0550c, (MTCY25D10.29c), len: 88 aa. Possible vapB3, antitoxin, part of toxin-antitoxin (TA) operon with Rv0549c (See Arcus et al., 2005; Pandey and Gerdes, 2005). P9WJ59 No virulence, detoxification, adaptation O06416 P9WJ59 Mb0565c NC_000962.3 Mycobrowser_v4 CDS 658321 659340 . + 0 Rv0567 Rv0567 Causes methylation Probable methyltransferase/methylase Rv0567, (MTV039.05), len: 339 aa. Probable methyltransferase, similar to several e.g. P39896|TCMO_STRGA tetracenomycin polyketide synthesis 8-O-methyltransferase from Streptomyces glaucescens (339 aa), FASTA scores: opt: 685, E(): 0, (35.8% identity in 335 aa overlap); P10950|HIOM_BOVIN hydroxyindole O-methyltransferase from Bos taurus (345 aa), FASTA scores: opt: 509, E(): 3.4e-27, (30.7% identity in 332 aa overlap) etc. O53764 No intermediary metabolism and respiration O53764 O53764 GO:0008171 2.1.1.- O53764 MMAR_0934 Mb0582 NC_000962.3 Mycobrowser_v4 CDS 642889 644493 . + 0 Rv0552 Rv0552 Function unknown Conserved protein Rv0552, (MTCY25D10.31), len: 534 aa. Conserved protein, similar to others from several organisms. Also shows some similarity with regulatory proteins e.g. AEPA_ERWCA|Q06555 exoenzymes regulatory protein aepA [Precursor] from Erwinia carotovora (465 aa), FASTA scores: opt: 278, E(): 7.6e-11, (23.0% identity in 408 aa overlap). Also similar to Z99119|BSUB0016_28 from Bacillus subtilis (529 aa), FASTA scores: opt: 436, E(): 8.3e-20, (23.8% identity in 547 aa overlap). C-terminus is similar to MLRRNOPR_1 hypothetical 17.7 kDa protein from Mycobacterium leprae (154 aa), FASTA score: (43.1% identity in 160 aa overlap). O06418 No conserved hypotheticals O06418 O06418 GO:0016810 O06418 ML2267 Mb0567 NC_000962.3 Mycobrowser_v4 CDS 644490 645470 . + 0 Rv0553 menC Possibly involved in menaquinone biosynthesis. Catalyzes a syn cycloisomerization [catalytic activity: 2,5-dihydro-5-oxofuran-2-acetate = cis,cis-hexadienedioate]. Probable muconate cycloisomerase MenC (cis,cis-muconate lactonizing enzyme) (MLE) Rv0553, (MTCY25D10.32), len: 326 aa. Probable menC, muconate cycloisomerase, equivalent to NP_302476.1|NC_002677 putative isomerase/racemase from Mycobacterium leprae (334 aa). Also similar to other muconate cycloisomerases e.g. TCBD_PSESP|P27099 chloromuconate cycloisomerase (370 aa), FASTA scores: opt: 249, E(): 7.8e-09, (32.7% identity in 199 aa overlap). Also similar to O-succinylbenzoate-CoA synthases. Belongs to the mandelate racemase / muconate lactonizing enzyme family. P9WJP3 No intermediary metabolism and respiration P65425 P9WJP3 GO:0009234,GO:0046872,GO:0016836 4.2.1.113 P9WJP3 ML2268 MMAR_0899 MSMEG_1103 Mb0568 NC_000962.3 Mycobrowser_v4 CDS 645467 646255 . + 0 Rv0554 bpoC Supposedly involved in detoxification reactions. Possible peroxidase BpoC (non-haem peroxidase) Rv0554, (MTCY25D10.33), len: 262 aa. Possible bpoC, peroxidase (non-haem peroxidase), equivalent to NP_302477.1|NC_002677 putative hydrolase from Mycobacterium leprae (265 aa). Also highly similar or similar to various hydrolases and peroxidases e.g. CAB38877.1|AL035707|T36181 probable hydrolase from Streptomyces coelicolor (272 aa); CAC48368.1|Y16952 putative hydrolase from Amycolatopsis mediterranei (284 aa); P29715|BPA2_STRAU non-haem bromoperoxidase bpo-a2 (bromide peroxidase) from Streptomyces aureofaciens (277 aa), FASTA scores: opt: 325, E(): 2.3e-15, (29.5% identity in 268 aa overlap); O31168|PRXC_STRAU|CPO|CPOT non-heme chloroperoxidase (chloride peroxidase) from Streptomyces aureofaciens (278 aa); etc. Also similar to M. tuberculosis non-heme haloperoxidases and epoxide hydrolases e.g. Rv1938, Rv3617, etc. P9WNH1 No virulence, detoxification, adaptation 3E3A,3HSS,3HYS,3HZO O06420 P9WNH1 GO:0004601,GO:0055114 1.11.1.- P9WNH1 ML2269 MMAR_0900 MSMEG_1108 Mb0569 NC_000962.3 Mycobrowser_v4 CDS 651529 652755 . - 0 Rv0561c Rv0561c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0561c, (MTCY25D10.40c), len: 408 aa. Possible oxidoreductase, highly similar (except in first 30 aa) to NP_302482.1|NC_002677 putative FAD-linked oxidoreductase from Mycobacterium leprae (408 aa). Also similar to T34627 probable electron transfer oxidoreductase from Streptomyces coelicolor (430 aa); and some bacteriochlorophyll synthases e.g. NP_069300.1|NC_000917 bacteriochlorophyll synthase from Archaeoglobus fulgidus (410 aa); Q55087 geranylgeranyl hydrogenase (407 aa), FASTA scores: opt: 208, E(): 1.7e-06, (26.9% identity in 327 aa overlap). P9WNY9 No intermediary metabolism and respiration O06427 P9WNY9 GO:0055114,GO:0004497 1.-.-.- P9WNY9 ML2276,ML2276c MMAR_0910 MSMEG_1132 Mb0576c NC_000962.3 Mycobrowser_v4 CDS 677125 677616 . - 0 Rv0580c Rv0580c Function unknown Conserved protein Rv0580c, (MTV039.18c), len: 163 aa. Conserved protein, equivalent to AAA90989.1|U20446|MK35 lipoprotein precursor from Mycobacterium kansasii (225 aa). O53777 No conserved hypotheticals O53777 O53777 ML1916 Mb0595c NC_000962.3 Mycobrowser_v4 CDS 1214513 1214947 . + 0 Rv1088 PE9 Function unknown PE family protein PE9 Rv1088, (MTV017.41), len: 144 aa. PE9, Member of Mycobacterium tuberculosis PE family (see citation below), similar to many others e.g. Z96071|MTCI418B_6 Mycobacterium tuberculosis cosmid (487 aa), FASTA scores: opt: 318, E(): 7.3e-14, (60.9% identity in 87 aa overlap) - except it appears to be frameshifted around codon 84. No error to account for frameshift could be found. Q79FS8 No PE/PPE Q79FS8 Q79FS8 Q79FS8 Mb1117 NC_000962.3 Mycobrowser_v4 CDS 1265087 1265455 . - 0 Rv1137c Rv1137c Unknown Hypothetical protein Rv1137c, (MTCI65.04c), len: 122 aa. Hypothetical unknown protein. O06537 No conserved hypotheticals O06537 Mb1169c NC_000962.3 Mycobrowser_v4 CDS 647959 648474 . + 0 Rv0556 Rv0556 Unknown Probable conserved transmembrane protein Rv0556, (MTCY25D10.35), len: 171 aa. Probable conserved transmembrane protein, equivalent to NP_302479.1|NC_002677 putative membrane protein from Mycobacterium leprae (175 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06422 No cell wall and cell processes O06422 GO:0016021 ML2271 MMAR_0902 MSMEG_1110 Mb0571 NC_000962.3 Mycobrowser_v4 CDS 648536 649672 . + 0 Rv0557 mgtA Involved in lipomannan (LM) biosynthesis Mannosyltransferase MgtA Rv0557, (MTCY25D10.36), len: 378 aa. MgtA (previously known as pimB), mannosyltransferase (see citation below), similar to other various transferases e.g. NP_243554.1|NC_002570 alpha-D-mannose-alpha(1-6)phosphatidyl myo-inositol monomannoside transferase from Bacillus halodurans (381 aa); NP_249533.1|NC_002516 probable glycosyl transferase from Pseudomonas aeruginosa (406 aa); NP_419573.1|NC_002696 glycosyl transferase, group 1 family protein, from Caulobacter crescentus (455 aa); etc. Also similar to Q55598 hypothetical 44.9 kDa protein from synechocystis SP (409 aa), FASTA scores: opt: 703, E(): 0, (33.9% identity in 378 aa overlap); GPI3_YEAST|P32363 n-acetylglucosaminyl-phosphatidylinositol biosynthetic protein (452 aa), FASTA scores: opt: 230, E(): 1.1e-07, (23.5% identity in 328 aa overlap). P9WMY5 No lipid metabolism O06423 P9WMY5 GO:0008654,GO:0016757,GO:0009405 2.4.1.- P9WMY5 ML2272 MMAR_0903 MSMEG_1113 Mb0572 NC_000962.3 Mycobrowser_v4 CDS 652771 653778 . + 0 Rv0562 grcC1 Possibly supplies polyprenyl diphosphate. Probable polyprenyl-diphosphate synthase GrcC1 (polyprenyl pyrophosphate synthetase) Rv0562, (MTCY25D10.41), len: 335 aa. Probable grcC1, polyprenyl diphosphate synthetase, equivalent to NP_302483.1|NC_002677 polyprenyl diphosphate synthase component from Mycobacterium leprae (330 aa). Also similar to others (generally hepta or hexaprenyl) e.g. GRC3_BACSU|P31114 probable heptaprenyl diphosphate syntetase (348 aa), FASTA scores: opt: 599, E(): 4e-31, (33.2% identity in 307 aa overlap); etc. Also highly similar to Mycobacterium tuberculosis proteins Rv0989c|grcC2|NP_215504.1|MTCI237.03c probable polyprenyl-diphosphate synthase (325 aa); Rv3383c, Rv3398c, etc. Contains PS00444 Polyprenyl synthetases signature 2. Belongs to the FPP/GGPP synthetases family. O06428 No intermediary metabolism and respiration O06428 O06428 GO:0016740,GO:0008299 2.5.1.- O06428 ML2277 MMAR_0911 MSMEG_1133 Mb0577 NC_000962.3 Mycobrowser_v4 CDS 654924 655949 . - 0 Rv0564c gpdA1 Involved in de novo phospholipid biosynthesis; glycerol-3 phosphate formation [catalytic activity: SN-glycerol 3-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H]. Probable glycerol-3-phosphate dehydrogenase [NAD(P)+] GpdA1 (NAD(P)H-dependent glycerol-3-phosphate dehydrogenase) (NAD(P)H-dependent dihydroxyacetone-phosphate reductase) Rv0564c, (MTV039.02c), len: 341 aa. Possible gpdA1(alternate gene names: gpsA, glyC), glycerol-3-phosphate dehydrogenase [NAD(P)+] dependent, similar to many other glycerol-3-phosphate dehydrogenases e.g. P46919|GPDA_BACSU from Bacillus subtilis (345 aa), FASTA scores: opt: 731, E(): 0, (37.3% identity in 332 aa overlap); etc. Also similar to Rv2982c|gpdA2|MTCY349.05|Z83018|MTCY349_5 from Mycobacterium tuberculosis (334 aa), FASTA scores: opt: 740, E(): 0, (40.4% identity in 322 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family. P9WN75 No lipid metabolism P64188 P9WN75 GO:0046167,GO:0046168,GO:0004367,GO:0047952,GO:0009331,GO:0055114,GO:0008654,GO:0051287 1.1.1.94 P9WN75 ML2280,ML2280c MMAR_0915 MSMEG_1140 Mb0579c NC_000962.3 Mycobrowser_v4 CDS 656010 657470 . - 0 Rv0565c Rv0565c Function unknown; probably involved in cellular metabolism. Probable monooxygenase Rv0565c, (MTV039.03c), len: 486 aa. Probable monoxygenase, highly similar to NP_301173.1|NC_002677 putative monooxygenase from Mycobacterium leprae (494 aa). Also highly similar to others e.g. NP_421371.1|NC_002696 monooxygenase (flavin-binding family) from Caulobacter crescentus (498 aa); C-terminus of NP_051574.1|NC_000958 arylesterase/monoxygenase from Deinococcus radiodurans (833 aa); P12015|CYMO_ACISP cyclohexanone monooxygenase from Acinetobacter sp. (542 aa), FASTA scores: opt: 354, E(): 2.1e-16, (23.7% identity in 435 aa overlap); etc. Also similar to other putative monoxygenases from Mycobacterium tuberculosis e.g. Rv3854c (489 aa), MTCY01A6.14 (489 aa), MTV013_4 (495 aa), MTCY31.20 (495 aa). O53762 No intermediary metabolism and respiration O53762 O53762 GO:0050661,GO:0004499,GO:0055114,GO:0050660 1.14.-.- O53762 ML2281,ML2281c MMAR_0917 MSMEG_2038 Mb0580c NC_000962.3 Mycobrowser_v4 CDS 657548 658039 . - 0 Rv0566c Rv0566c Function unknown Conserved protein Rv0566c, (MTV039.04c), len: 163 aa. Conserved protein, similar to others e.g. P77482|YAJQ_ECOLI hypothetical 19.0 KDa protein from Escherichia coli (169 aa), FASTA scores: opt: 422, E(): 5.4e-20, (44.1 identity in 161 aa overlap); etc. P9WFK9 No conserved hypotheticals P67380 P9WFK9 P9WFK9 ML2282,ML2282c MMAR_0920 MSMEG_1165 Mb0581c NC_000962.3 Mycobrowser_v4 CDS 667252 668394 . - 0 Rv0574c Rv0574c Function unknown Conserved hypothetical protein Rv0574c, (MTV039.12c), len: 380 aa. Conserved hypothetical protein, showing similarity with other hypothetical proteins and polyglutamate synthases (encapsulation proteins) e.g. AAK64444.1|AF377339_5|AF377339 polyglutamate synthase CapA from Myxococcus xanthus (405 aa); M24150|BACCAPABC_3|CapA polyglutamate synthase (encapsulation protein) from B.anthracis (411 aa), FASTA scores: opt: 261, E(): 4.3e-10, (25.8% identity in 287 aa overlap); etc. P9WM79 No conserved hypotheticals O53771 P9WM79 P9WM79 ML2105,ML2105c Mb0589c NC_000962.3 Mycobrowser_v4 CDS 676238 676996 . + 0 Rv0579 Rv0579 Function unknown Conserved hypothetical protein Rv0579, (MTV039.17), len: 252 aa. Conserved hypothetical protein, showing some similarity to others e.g. AE001747_4 hypothetical protein from Thermotoga maritima (247 aa), FASTA scores: opt: 612, E(): 0, (39.6% identity in 235 aa overlap); AE001004_2 hypothetical protein from Archaeoglobus fulgidus (159 aa), FASTA scores: opt: 196, E(): 1e-06, (28.3% identity in 159 aa overlap); etc. O53776 No conserved hypotheticals O53776 O53776 O53776 MMAR_0946 Mb0594 NC_000962.3 Mycobrowser_v4 CDS 2546488 2546805 . - 0 Rv2274c mazF8 Unknown Possible toxin MazF8 Rv2274c, (MTCY339.36), len: 105 aa. Possible mazF8, toxin, part of toxin-antitoxin (TA) operon with Rv2274A (See Pandey and Gerdes, 2005). Questionable ORF. P9WIH7 No virulence, detoxification, adaptation P9WIH7 P9WIH7 Mb2297c NC_000962.3 Mycobrowser_v4 CDS 661003 661269 . + 0 Rv0569 Rv0569 Function unknown Conserved protein Rv0569, (MTV039.07), len: 88 aa. Conserved protein. C-terminus highly similar to AAA63065.1|U15184|MLU15184_10 hypothetical protein from Mycobacterium leprae (53 aa), FASTA scores: opt: 140, E(): 0.0046, (64.7% identity in 34 aa overlap). Also similar to T36824|SCI35.11 hypothetical protein from Streptomyces coelicolor (64 aa); and N-terminus of T36956 probable DNA-binding protein from Streptomyces coelicolor (323 aa). Also highly similar to Rv2302|MTCY339.07c|NP_216818.1|NC_000962 conserved hypothetical protein from Mycobacterium tuberculosis (80 aa), FASTA scores: opt: 300, E(): 1.4e-13, (61.8% identity in 76 aa overlap). P9WM83 No conserved hypotheticals O53766 P9WM83 P9WM83 ML2100,ML2100c Mb0584 NC_000962.3 Mycobrowser_v4 CDS 663487 664818 . - 0 Rv0571c Rv0571c Function unknown Conserved protein Rv0571c, (MTV039.09c), len: 443 aa. Conserved protein, highly similar to the products of two adjacent orfs in Mycobacterium leprae: AAA63059.1|U15184|U650S|Q50111 hypothetical protein (258 aa), FASTA scores: opt: 1071, E(): 0, (72.5% identity in 233 aa overlap); and AAA63058.1|U15184|U650T hypothetical protein (86 aa), FASTA scores: opt: 192, E(): 6.4e-06, (70.8% identity in 48 aa overlap). Also similar to others e.g. NP_107072.1|NC_002678 hypothetical protein from Mesorhizobium loti (235 aa); NP_213031.1|NC_000918 hypothetical protein from Aquifex aeolicus (175 aa); etc. And similar to part of hypothetical proteins from Mycobacterium tuberculosis e.g. C-terminus of Rv2143|MTCY270.25c|Z95388|NP_216659.1|NC_000962 (352 aa), FASTA scores: opt: 592, E(): 7e-32, (49.3% identity in 205 aa overlap); N-terminus of Rv2030c|NP_216546.1|NC_000962 (681 aa). P9WHK1 No conserved hypotheticals O53768 P9WHK1 GO:0009116,GO:0016740,GO:0016787 P9WHK1 ML2106 Mb0586c NC_000962.3 Mycobrowser_v4 CDS 679229 681862 . + 0 Rv0584 Rv0584 Unknown Possible conserved exported protein Rv0584, (MTV039.22), len: 877 aa. Possible conserved exported protein, similar to other hypothetical proteins which are not necessarily secreted e.g. CAB61925.1|AL133278 putative secreted protein from Streptomyces coelicolor (772 aa); AAD51075.1|AF175722_1|AF175722 immunoreactive 89kD antigen PG87 from Porphyromonas gingivalis (781 aa), FASTA scores: opt: 637, E(): 2.1e-30, (29.1% identity in 794 aa overlap); etc. Contains PS00699 Nitrogenases component 1 alpha and beta subunits signature 1. Has potential N-terminal signal peptide. Predicted to be an outer membrane protein (See Song et al., 2008). O86365 No cell wall and cell processes O86365 O86365 O86365 MMAR_0952 Mb0599 NC_000962.3 Mycobrowser_v4 CDS 668579 669745 . - 0 Rv0575c Rv0575c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0575c, (MTV039.13c), len: 388 aa. Possible oxidoreductase, similar to many diverse oxidoreductases and monooxygenases e.g. AL109974|SCF34_5|T36404 probable monooxygenase from Streptomyces coelicolor (407 aa), FASTA scores: opt: 786, E(): 0, (38.7% identity in 398 aa overlap); P96555|AB000564 salicylate hydroxylase from sphingomonas (395 aa), FASTA scores: opt: 267, E():5e-11, (26.4% identity in 390 aa overlap). Also similar to Rv1260|Z77137|MTCY50.22C from Mycobacterium tuberculosis (372 aa), FASTA scores: opt: 762, E(): 0, (40.9% identity in 345 aa overlap). The transcription of this CDS seems to be activated in macrophages (see citation below). O53772 No intermediary metabolism and respiration O53772 O53772 GO:0055114,GO:0004497 1.-.-.- O53772 ML2287,ML2287c MMAR_0939 MSMEG_1980 Mb0590c NC_000962.3 Mycobrowser_v4 CDS 669848 671152 . + 0 Rv0576 Rv0576 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (possibly ArsR-family) Rv0576, (MTV039.14), len: 434 aa. Probable transcriptional regulator, ArsR family. N-terminus highly similar to others e.g. NP_102487.1|NC_002678 transcriptional regulator from Mesorhizobium loti (104 aa); NP_242952.1|NC_002570 transcriptional regulator (ArsR family) from Bacillus halodurans (109 aa); etc. C-terminal region (~240-434) shows similarity with D67028_1 from Rhodococcus rhodochrous (112 aa); and Rv0738 from Mycobacterium tuberculosis (182 aa). N-terminus also highly similar to Rv2034 from Mycobacterium tuberculosis (107 aa). Contains helix-turn-helix motif at aa 23-43 (Score 1628, +4.73 SD). O53773 No regulatory proteins O53773 O53773 GO:0006355,GO:0006950,GO:0006350,GO:0003700,GO:0005622 O53773 Mb0591 NC_000962.3 Mycobrowser_v4 CDS 671166 671951 . + 0 Rv0577 TB27.3 Function unknown. Involved in ability to absorb neutral red stain. Conserved protein TB27.3 Rv0577, (MTV039.15), len: 261 aa. TB27.3, conserved protein. Corresponds to O53774|CF30_MYCTU 27 kDa antigen CFP30B from Mycobacterium tuberculosis culture filtrate (260 aa), FASTA scores: opt: 1781, E(): 0, (100.0% identity in 260 aa overlap). Also similar to several hypothetical proteins and hydroxylases from Steptomyces sp. e.g. T35032 probable hydroxylase from Streptomyces coelicolor (263 aa); Q55078 orfA gene product from Streptomyces sp. (275 aa), FASTA scores: E(): 1.5e-1 9, (38.6% identity in 264 aa overlap); D89734_1|P95754 DNA for SgaA SGAA protein from Streptomyces griseus; and SC9B10_20 from Streptomyces coelicolor (267 aa), FASTA score: (38.9 identity in 252 aa overlap). Also similar to Rv0911|MTCY21C12.05 from Mycobacterium tuberculosis (257 aa), FASTA scores: E(): 1.1e-20, (32.0% identity in 259 aa overlap). P9WIR3 No conserved hypotheticals 3OXH P0A5N8 P9WIR3 P9WIR3 MMAR_3576 Mb0592 NC_000962.3 Mycobrowser_v4 CDS 1981130 1981579 . + 0 Rv1752 Rv1752 Function unknown Conserved hypothetical protein Rv1752, (MTCY28.15), len: 149 aa. Conserved hypothetical protein, similar to C-terminal half of Q9TV68|AB021930|CAN2DD Dihydrodiol dehydrogenase from Canis familiaris (335 aa), FASTA score, opt: 168, E(): 0.00015, (31.3% identity in 112 aa overlap). O06789 No conserved hypotheticals O06789 O06789 Mb1781 NC_000962.3 Mycobrowser_v4 CDS 684410 685132 . + 0 Rv0586 mce2R Involved in transcriptional mechanism. Probable transcriptional regulatory protein Mce2R (GntR-family) Rv0586, (MTCY19H5.36c), len: 240 aa. Probable mce2R, transcriptional regulator, GntR family, part of mce2 operon, similar to many e.g. P33233|LLDR_ECOLI putative L-lactate dehydrogenase operon regulatory protein from Escherichia coli (258 aa), FASTA scores: opt: 225, E(): 9.3e-08, (26.7% identity in 232 aa overlap); etc. Also similar to other M. tuberculosis transcriptional regulators GntR proteins e.g. Rv3060c, Rv0792c, etc. Contains PS00043 Bacterial regulatory proteins, gntR family signature and probable helix-turn helix motif from aa 35-56 (Score 1531, +4.40 SD). P9WMG5 No regulatory proteins P67741 P9WMG5 GO:0006355,GO:0006350,GO:0003700,GO:0005622 P9WMG5 MSMEG_3527 Mb0601 NC_000962.3 Mycobrowser_v4 CDS 685129 685926 . + 0 Rv0587 yrbE2A Unknown Conserved hypothetical integral membrane protein YrbE2A Rv0587, (MTCY19H5.35c), len: 265 aa. YrbE2A, hypothetical unknown integral membrane protein, part of mce2 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07412|Rv0167|MTCI28.07|yrbE1A (265 aa); O53965|Rv1964|MTV051.02|yrbE3A (265 aa); etc. Also highly similar to conserved hypothetical integral membrane proteins of the yrbEA type, e.g. P45392|YRBE_ECOLI hypothetical 27.9 kDa protein from Escherichia coli (260 aa), FASTA scores: opt: 287, E(): 6.1e-12, (21.5% identity in 256 aa overlap); P45030|YRBE_HAEIN|HI1086 hypothetical protein from Haemophilus influenzae (261 aa), FASTA scores: opt: 311, E(): 1.8e-83, (24.2% identity in 265 aa overlap); NP_302654.1|NC_002677 conserved membrane protein from Mycobacterium leprae (267 aa); etc. I6Y870 No virulence, detoxification, adaptation O07791 I6Y870 MSMEG_0132 Mb0602 NC_000962.3 Mycobrowser_v4 CDS 699038 699799 . - 0 Rv0602c tcrA Sensor part of a two component regulatory system. Two component DNA binding transcriptional regulatory protein TcrA Rv0602c, (MTCY19H5.20), len: 253 aa. tcrA, two-component DNA-binding response regulator, highly similar to others e.g. NP_107959.1|NC_002678 two-component response regulator from Mesorhizobium loti (239 aa); etc. Also similar to many other Mycobacterium tuberculosis two-component regulators e.g. Q50806|MTCY10G2.16|Rv1033c response regulator homolog TRCR (TCRV) (257 aa), FASTA score: (47.4 identity in 232 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07776 No regulatory proteins O07776 O07776 GO:0005737,GO:0006355,GO:0006350,GO:0000156,GO:0000160,GO:0003677 O07776 Mb0618c NC_000962.3 Mycobrowser_v4 CDS 817531 817866 . - 0 Rv0724A Rv0724A Function unknown Conserved hypothetical protein Rv0724A, len: 111 aa. Similarity suggests that this CDS should be continuation of Rv0725c but we can find no frame-shift to account for this. Possible extended protein is very similar to other hypothetical Mycobacterium tuberculosis proteins e.g. Rv1729c|Z81360_12 (312 aa), FASTA scores: opt: 399, E(): 2e-19, (58.7% identity in 109 aa overlap); Rv0731c, Rv0726c, etc. Frame-shift could occur at nt 817866. Same sequence for strain CDC1551 and Mycobacterium bovis. Q79FX1 No conserved hypotheticals Q79FX1 Q79FX1 NC_000962.3 Mycobrowser_v4 CDS 698524 698994 . - 0 Rv0601c Rv0601c Sensor part of a two component regulatory system. Two component sensor kinase [first part] Rv0601c, (MTCY19H5.21), len: 156 aa (probable partial CDS). Two-component sensor kinase (first part), similar to part (N-termini) of others e.g. Q0375|CUTS_STRLI cuts protein from streptomyces lividans (414 aa), FASTA scores: opt: 230, E(): 3.1e-08, (39.1% identity in 115 aa overlap). Note that the sequence was checked and no errors were detected that would allow this and the downstream ORF to be joined. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07777 No regulatory proteins O07777 O07777 GO:0005886,GO:0007165,GO:0000155,GO:0000160,GO:0016021 2.7.13.3 Mb0617c NC_000962.3 Mycobrowser_v4 CDS 1419961 1420302 . - 0 Rv1271c Rv1271c Unknown Conserved hypothetical secreted protein Rv1271c, (MTCY50.11), len: 113 aa. Conserved hypothetical exported protein with potential N-terminal signal sequence. Similar to Mycobacterium tuberculosis hypothetical proteins Rv1804c, Rv1810, Rv0622, etc. P9WM43 No cell wall and cell processes P64793 P9WM43 P9WM43 MMAR_4150,MMAR_4151 Mb1302c NC_000962.3 Mycobrowser_v4 CDS 702813 703199 . + 0 Rv0607 Rv0607 Unknown Hypothetical protein Rv0607, (MTCY19H5.15c), len: 128 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07771 No conserved hypotheticals O07771 Mb0623 NC_000962.3 Mycobrowser_v4 CDS 703244 703489 . + 0 Rv0608 vapB28 Unknown Possible antitoxin VapB28 Rv0608, (MTCY19H5.14c), len: 81 aa. Possible vapB28, antitoxin, part of toxin-antitoxin (TA) operon with Rv0609, see Arcus et al. 2005. Similar to several others e.g. Rv0623|P96913|MTCY20H10.04 (84 aa), FASTA scores: opt: 159, E(): 1.2e-09, (43.0% identity in 86 aa overlap); Rv2760c (89 aa); Rv1740 (70 aa), etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WJ39 No virulence, detoxification, adaptation O07770 P9WJ39 Mb0624 NC_000962.3 Mycobrowser_v4 CDS 703830 704057 . + 0 Rv0609A Rv0609A Function unknown Conserved hypothetical protein Rv0609A, len: 75 aa. Conserved hypothetical protein, highly similar to part of upstream ORF Rv0612|MTCY19H5.09c conserved hypothetical protein from Mycobacterium tuberculosis (201 aa), FASTA scores: opt: 154, E(): 1.8e-05, (74.3% identity in 35 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q79FY5 No conserved hypotheticals Q79FY5 Mb0626 NC_000962.3 Mycobrowser_v4 ncRNA 704187 704247 . + 0 MTB000052 B55 Unknown Putative small regulatory RNA B55, putative small regulatory RNA (See Arnvig and Young, 2009). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). No stable RNAs NC_000962.3 Mycobrowser_v4 CDS 711006 711407 . + 0 Rv0617 vapC29 Unknown Possible toxin VapC29. Contains PIN domain. Rv0617, (MTCY19H5.04c), len: 133 aa. Possible vapC29, toxin, part of toxin-antitoxin (TA) operon with Rv0616A, contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. Rv2494, Rv3320c, Rv0749, Rv0277c, Rv2530c, etc. P9WF79 No virulence, detoxification, adaptation O07760 P9WF79 P9WF79 Mb0634 NC_000962.3 Mycobrowser_v4 CDS 2545332 2545631 . + 0 Rv2271 Rv2271 Unknown Conserved hypothetical protein Rv2271, (MTCY339.39c), len: 99 aa. Conserved hypothetical protein; some similarity to hypothetical protein AAK01340.1|AF265275_3 (AF265275) from uncultured organism Pu8 (104 aa) E= 4e-10, (34% identity in 91 aa overlap) P9WLF7 No conserved hypotheticals P9WLF7 Mb2294 NC_000962.3 Mycobrowser_v4 CDS 710584 710850 . - 0 Rv0616c Rv0616c Unknown Hypothetical protein Rv0616c, (MTCY19H5.05), len: 88 aa. Hypothetical unknown protein. O07761 No conserved hypotheticals O07761 Mb0633c NC_000962.3 Mycobrowser_v4 CDS 711536 712231 . + 0 Rv0618 galTa Involved in galactose metabolism (leloir pathway) [catalytic activity: UTP + alpha-D-galactose 1-phosphate = diphosphate + UDP-galactose]. Probable galactose-1-phosphate uridylyltransferase GalTa [first part] Rv0618, (MTCY19H5.03c), len: 231 aa (probable partial CDS). Probable galTa, first part of galactose-1-phosphate uridylyltransferase, highly similar to N-terminal half of other galT proteins e.g. P13212|GAL7_STRLI galactose-1-phosphate uridylyltransferase from Streptomyces lividans (354 aa), FASTA scores: opt: 296, E(): 1.4e-11, (50.8% identity in 177 aa overlap); etc. Also highly similar to N-terminal half of some UDP glucose--hexose-1-phosphate uridylyltransferases. N-terminal 28 aa similar to MTCY20H11.08|Rv0627|MTCY20H11.08 conserved hypothetical protein from Mycobacterium tuberculosis (135 aa), FASTA score: (71.4% identity in 28 overlap). Cosmid sequence is correct but there may be a frameshift mutation in this region which would allow the two ORFs to be joined. Belongs to the galactose-1-phosphate uridylyltransferase family 1. Note that previously known as galT'. Q79FY4 No intermediary metabolism and respiration Q79FY4 Q79FY4 GO:0017103,GO:0006012,GO:0008270,GO:0008108 2.7.7.10 Q79FY4 Mb0635 NC_000962.3 Mycobrowser_v4 CDS 712174 712719 . + 0 Rv0619 galTb Involved in galactose metabolism (leloir pathway) [catalytic activity: UTP + alpha-D-galactose 1-phosphate = diphosphate + UDP-galactose]. Probable galactose-1-phosphate uridylyltransferase GalTb [second part] Rv0619, (MTCY19H5.02c), len: 181 aa (probable partial CDS). Probable galTb, second part of galactose-1-phosphate uridylyltransferase, highly similar to C-terminal half of other galT proteins e.g. P13212|GAL7_STRLI galactose-1-phosphate uridylyltransferase from Streptomyces lividans (354 aa), FASTA scores: opt: 416, E(): 5.2e-22, (43.0% identity in 186 aa overlap), etc. Cosmid sequence is correct but there may be a frameshift mutation in this region which would allow the two ORFS to be joined. Belongs to the galactose-1-phosphate uridylyltransferase family 1. Note that previously known as 'galT. Q79FY3 No intermediary metabolism and respiration Q79FY3 Q79FY3 GO:0017103,GO:0006012,GO:0008270,GO:0008108 2.7.7.10 Q79FY3 Mb0635 NC_000962.3 Mycobrowser_v4 CDS 729327 730166 . - 0 Rv0633c Rv0633c Unknown Possible exported protein Rv0633c, (MTCY20H11.14c), len: 279 aa. Possible exported protein; has hydrophobic stretch at aa 23-41. P96923 No cell wall and cell processes P96923 Mb0650c NC_000962.3 Mycobrowser_v4 CDS 828140 828892 . + 0 Rv0736 rslA Regulates negatively SIGL|Rv0735 Anti-sigma factor RslA Rv0736, (MTV041.10), len: 250 aa. RslA, anti-sigma factor (See Dainese et al., 2006). Probable membrane protein, showing weak similarity with AL133469|SCM10_32 putative membrane protein from Streptomyces coelicolor (216 aa), FASTA scores: opt: 180, E(): 0.00018, (34.3% identity in 216 aa overlap). Cleaved by Rip|Rv2869c, in M. tuberculosis Erdman (See Sklar et al., 2010). P9WJ67 No information pathways 3HUG P9WJ67 P9WJ67 MMAR_1074 MSMEG_1487 Mb0757 NC_000962.3 Mycobrowser_v4 CDS 1445058 1445372 . + 0 Rv1290A Rv1290A Unknown Hypothetical protein Rv1290A, len: 104 aa. Hypothetical unknown protein, equivalent to AAK45590 from Mycobacterium tuberculosis strain CDC1551 (122 aa) but shorter 18 aa. Q79FQ6 No conserved hypotheticals Q79FQ6 Mb1322 NC_000962.3 Mycobrowser_v4 CDS 718282 718689 . + 0 Rv0627 vapC5 Unknown Possible toxin VapC5 Rv0627, (MTCY20H11.08), len: 135 aa. Possible vapC5, toxin, part of toxin-antitoxin (TA) operon with Rv0626, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. Rv0595c and Rv0665. P96917 No virulence, detoxification, adaptation 3DBO P96917 P96917 P96917 Mb0643 NC_000962.3 Mycobrowser_v4 CDS 714202 715266 . + 0 Rv0621 Rv0621 Unknown Possible membrane protein Rv0621, (MTCY20H10.02), len: 354 aa. Possible membrane protein; contains potential membrane spanning regions. Also contains PS00017 ATP/GTP-binding site motif A (P-loop). I6X9F4 No cell wall and cell processes I6X9F4 I6X9F4 Mb0637 NC_000962.3 Mycobrowser_v4 CDS 716410 716664 . + 0 Rv0623 vapB30 Unknown Possible antitoxin VapB30 Rv0623, (MTCY20H10.04), len: 84 aa. Possible vapB30, antitoxin, part of toxin-antitoxin (TA) operon with Rv0624, see Arcus et al. 2005. Also similar to others in Mycobacterium tuberculosis e.g MTCY28_2|Rv1740|MTCY28.02|MTCY04C12.25 conserved hypothetical protein (70 aa), FASTA score: (73.5% identity in 68 aa overlap); MTCY4C12_25|Rv0608|MTCY19H5.14c conserved hypothetical protein (81 aa), FASTA score: (73.5 identity in 68 aa overlap); etc. P9WJ35 No virulence, detoxification, adaptation P96913 P9WJ35 P9WJ35 MSMEG_1283 Mb0639 NC_000962.3 Mycobrowser_v4 CDS 716664 717059 . + 0 Rv0624 vapC30 Unknown Possible toxin VapC30. Contains PIN domain. Rv0624, (MTCY20H10.05), len: 131 aa. Possible vapC30, toxin, part of toxin-antitoxin (TA) operon with Rv0623, contains PIN domain, see Arcus et al. 2005. Highly similar to others in Mycobacterium tuberculosis e.g. Rv1741, Rv0609, Rv2759c,Rv0565c, Rv3854c, Rv3083, etc. P9WF77 No virulence, detoxification, adaptation P67240 P9WF77 P9WF77 MSMEG_1284 Mb0640 NC_000962.3 Mycobrowser_v4 CDS 717153 717893 . - 0 Rv0625c Rv0625c Unknown Probable conserved transmembrane protein Rv0625c, (MTCY20H10.06c), len: 246 aa. Probable conserved transmembrane protein, showing similarity with others e.g. CAB61866.1|AL133252 putative integral membrane protein from Streptomyces coelicolor (249 aa). Also similar to Rv1491c|MTCY277_13 from Mycobacterium tuberculosis. Contains potential membrane spanning regions. P9WFS5 No cell wall and cell processes P67115 P9WFS5 GO:0005886,GO:0016021 MMAR_3541 MSMEG_2840 Mb0641c NC_000962.3 Mycobrowser_v4 CDS 718025 718285 . + 0 Rv0626 vapB5 Possible ribonuclease Possible antitoxin VapB5 Rv0626, (MTCY20H10.07), len: 86 aa. Possible vapB5, antitoxin, part of toxin-antitoxin (TA) operon with Rv0627 (See Arcus et al., 2005; Pandey and Gerdes, 2005)., similar to others in Mycobacterium tuberculosis hypothetical proteins e.g. Rv0596c, Rv3385c, Rv3407,Rv3181c, etc. Cofactor: Mg2+ P9WF19 No virulence, detoxification, adaptation 3DBO P96916 P9WF19 P9WF19 Mb0642 NC_000962.3 Mycobrowser_v4 CDS 718761 719912 . - 0 Rv0628c Rv0628c Function unknown Conserved hypothetical protein Rv0628c, (MTCY20H10.09c), len: 383 aa. Conserved hypothetical protein, highly similar to Rv0874c|YZ02_MYCTU|Q10536 conserved hypothetical protein from Mycobacterium tuberculosis (386 aa), FASTA scores: opt: 2082, E(): 0, (81.5% identity in 383 aa overlap). Also some similarity to P72543|SPU62616_1 hypothetical protein from Synechococcus, FASTA scores: E(): 2.8e-28, (36.6 identity in 265 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WKS7 No conserved hypotheticals P64729 P9WKS7 GO:0005886,GO:0016021 P9WKS7 Mb0644c NC_000962.3 Mycobrowser_v4 CDS 732393 732821 . + 0 Rv0636 hadB Involved in fatty acid synthesis type II (fas-II) (3R)-hydroxyacyl-ACP dehydratase subunit HadB Rv0636, (MTCY20H10.17), len: 142 aa. HadB, (3R)-hydroxyacyl-ACP dehydratase subunit, equivalent to NP_302286.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (142 aa). Shows structural similarity to six others in Mycobacterium tuberculosis (see Castell et al (2005) below). Also highly similar to CAB77411.1|AL160431|SCD82.08 hypothetical protein from Streptomyces coelicolor (142 aa); and similar to others e.g. U28943|CELE04F6_3 from Caenorhabditis elegans (cosmid E04) (298 aa), FASTA scores: opt: 167, E(): 0.00064, (31.6 identity in 117 aa overlap). I6WYY7 No intermediary metabolism and respiration P96927 I6WYY7 GO:0016491,GO:0008152 I6WYY7 ML1909,ML1909c MMAR_0969 MSMEG_1341 Mb0655 NC_000962.3 Mycobrowser_v4 CDS 735022 735450 . + 0 Rv0640 rplK This protein binds directly to 23S ribosomal RNA. 50S ribosomal protein L11 RplK Rv0640, (MTCY20H11.21), len: 142 aa. rplK, 50S ribosomal protein L11, equivalent to NP_302282.1|NC_002677 50S ribosomal protein L11 from Mycobacterium leprae (142 aa). Also highly similar to others e.g. P48954|RL11_STRCO|SCD82.19 50s ribosomal protein L11 from Streptomyces coelicolor (144 aa), FASTA scores: opt: 763, E(): 0, (84.6% identity in 143 aa overlap); etc. Contains PS00359 Ribosomal protein L11 signature. Belongs to the L11P family of ribosomal proteins. P9WHE5 No information pathways P66056 P9WHE5 GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WHE5 ML1905,ML1905c MMAR_0973 MSMEG_1346 Mb0659 NC_000962.3 Mycobrowser_v4 CDS 721729 725013 . - 0 Rv0630c recB Involved in homologous recombination. Probable exonuclease V (beta chain) RecB (exodeoxyribonuclease V beta chain)(exodeoxyribonuclease V polypeptide) (chi-specific endonuclease) Rv0630c, (MTCY20H10.11c), len: 1094 aa. Probable recB, exonuclease V, beta chain (exodeoxyribonuclease V, beta chain) (see citation below), highly similar to other exonucleases e.g. AF157643_2|recB|AAD46808.1 Escherichia coli RecB protein homolog from Mycobacterium smegmatis (1083 aa); P08394|EX5B_ECOLI|RORA|B2820 exodeoxyribonuclease V 135 kDa polypeptide (exonuclease V beta chain) from Escherichia coli strain K12 (1180 aa), FASTA scores: opt: 289, E(): 4.3e-11, (29.5 identity in 1059 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the helicase family, UVRD subfamily. Consists of three subunits; RECB, RECC|Rv0631c and recd|Rv0629c. P9WMQ3 No information pathways P96920 P9WMQ3 GO:0004003,GO:0003677,GO:0006281,GO:0004519,GO:0008854,GO:0005524 3.1.11.5 P9WMQ3 MMAR_0957 MSMEG_1327 Mb0646c,Mb0647c NC_000962.3 Mycobrowser_v4 CDS 731930 732406 . + 0 Rv0635 hadA Involved in fatty acid synthesis type II (fas-II) (3R)-hydroxyacyl-ACP dehydratase subunit HadA Rv0635, (MTCY20H10.16), len: 158 aa. HadA, (3R)-hydroxyacyl-ACP dehydratase subunit, equivalent to NP_302287.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (159 aa); and highly similar to YV31_MYCLE|P54879 conserved hypothetical protein from Mycobacterium leprae (166 aa), FASTA scores: opt: 387, E(): 5.9e-21, (43.4% identity in 145 aa overlap). Also similar CAB77410.1|AL160431|SCD82.07 hypothetical protein from Streptomyces coelicolor (150 aa). And highly similar to two hypothetical proteins from Mycobacterium tuberculosis: Rv0504c|YV31_MYCTU|Q11168 (166 aa), FASTA scores: opt: 405, E(): 3.2e-22, (45.0% identity in 140 aa overlap); and Rv0637|MTY20H10_19 (2 ORFs downstream) (166 aa), FASTA score: (48.7% identity in 150 aa overlap). P9WFK1 No intermediary metabolism and respiration P9WFK1 P9WFK1 ML1910,ML1910c MMAR_0968 MSMEG_1340 Mb0654 NC_000962.3 Mycobrowser_v4 CDS 746363 747037 . + 0 Rv0649 fabD2 Involved in lipid metabolism; fatty acid biosynthesis [catalytic activity: malonyl-CoA + [acyl-carrier protein] = CoA + malonyl-[acyl-carrier protein]]. Possible malonyl CoA-acyl carrier protein transacylase FabD2 (MCT) Rv0649, (MTCY20H10.30), len: 224 aa. Possible fabD2, malonyl CoA-acyl carrier protein transacylase, similar to mtfabd|FABD_MYCTU|Q10501|Rv2243 malonyl CoA-acyl carrier protein transacylase from Mycobacterium tuberculosis (302 aa), FASTA scores: opt: 133, E(): 0.074, (31.3% identity in 147 aa overlap). Q79FX5 No lipid metabolism Q79FX5 GO:0004314 2.3.1.39 Q79FX5 Mb0668 NC_000962.3 Mycobrowser_v4 CDS 754685 754993 . - 0 Rv0659c mazF2 Function unknown Toxin MazF2 Rv0659c, (MTCI376.17), len: 102 aa. MazF2, toxin, part of toxin-antitoxin (TA) operon with Rv0660c (See Pandey and Gerdes, 2005; Zhu et al., 2006), weakly similar to other Mycobacterium tuberculosis hypothetical proteins e.g. Rv1942c, Rv1495, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WII1 No virulence, detoxification, adaptation O06780 P9WII1 GO:0003677 P9WII1 Mb0678c NC_000962.3 Mycobrowser_v4 CDS 1301307 1301747 . + 0 Rv1171 Rv1171 Unknown Conserved hypothetical protein Rv1171, (MTV005.07), len: 146 aa. Conserved hypothetical protein, possibly transmembrane protein. Start has been changed since first submission. O50427 No conserved hypotheticals O50427 ML1494,ML1494c MMAR_4281 MSMEG_5128 Mb1204 NC_000962.3 Mycobrowser_v4 tRNA 731494 731566 . + 0 MTB000006 thrT - tRNA-Thr (GGT) thrT, tRNA-Thr, anticodon ggt, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v4 tRNA 731603 731676 . + 0 MTB000007 metT - tRNA-Met (CAT) metT, tRNA-Met, anticodon cat, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v4 CDS 731712 731879 . + 0 Rv0634B rpmG2 Involved in translation mechanism 50S ribosomal protein L33 RpmG2 Rv0634B, len: 55 aa. rpmG2, 50S ribosomal protein L33. Note that Mycobacterium tuberculosis has a second rpmG gene: P96925|R33H_MYCTU|Rv2057c|MTCY63A.03|rpmG1 putative 50S ribosomal protein L33 (55 aa), FASTA scores: opt: 391, E(): 2.9e-25, (100.0% identity in 55 aa overlap). Belongs to the L33P family of ribosomal proteins. P9WH95 No information pathways P0A5W2 P9WH95 GO:0003735,GO:0006412,GO:0005840 P9WH95 ML1911,ML1911c Mb0653 NC_000962.3 Mycobrowser_v4 CDS 733737 734222 . + 0 Rv0638 secE1 Essential for protein export. Probable preprotein translocase SecE1 Rv0638, (MTCY20H10.19), len: 161 aa. Probable secE1, preprotein translocase (tail-anchored membrane protein) (see citation below), highly similar at C-terminal half to others e.g. P36690|SECE_STRGR preprotein translocase SECE subunit from Streptomyces griseus (86 aa), FASTA scores: opt: 220, E(): 4.6e-06, (35.4% identity in 96 aa overlap); P16920|SECE_ECOLI preprotein translocase sece subunit from Escherichia coli strains K12 and O157:H7 (127 aa), FASTA scores: opt: 122, E(): 0.34, (37.0% identity in 54 aa overlap); etc. Contains PS01067 Protein secE/sec61-gamma signature. Belongs to the SECE/SEC61-gamma family. Part of the prokaryotic protein translocation apparatus which comprise SECA|Rv3240c, SECD|Rv2587c, SECE, SECF|Rv2586c, SECG|Rv1440 and SECY|Rv0732. Note that previously known as secE. P9WGN7 No cell wall and cell processes P0A5Z0 P9WGN7 GO:0016021,GO:0005886,GO:0009306,GO:0006605,GO:0055085,GO:0015450 P9WGN7 ML1907,ML1907c MMAR_0971 MSMEG_1344 Mb0657 NC_000962.3 Mycobrowser_v4 CDS 734254 734970 . + 0 Rv0639 nusG Influences transcription termination and antitermination. Acts as a component of the transcription complex, and interacts with the termination factor rho and RNA polymerase. Probable transcription antitermination protein NusG Rv0639, (MTCY20H10.20), len: 238 aa. Probable nusG, transcription antitermination protein, equivalent to NP_302283.1|NC_002677 transcription antitermination protein nusG from Mycobacterium leprae (228 aa). Also highly similar to others e.g. P36260|NUSG_STRGR from Streptomyces griseus (294 aa), FASTA scores: opt: 845, E(): 0, (55.4% identity in 233 aa overlap); etc. Note that shorter at the N-terminus than other nusG. Contains PS01014 Transcription termination factor nusG signature. Belongs to the NusG family. P9WIU9 No information pathways P65589 P9WIU9 GO:0031564,GO:0003711,GO:0006353,GO:0032968 P9WIU9 ML1906,ML1906c MMAR_0972 MSMEG_1345 Mb0658 NC_000962.3 Mycobrowser_v4 CDS 2545737 2546105 . + 0 Rv2272 Rv2272 Unknown Probable conserved transmembrane protein Rv2272, (MTCY339.38c), len: 122 aa. Probable conserved transmembrane protein, similar to YIDH_ECOLI P31445 hypothetical 12.8 kDa protein (115 aa), FASTA scores, opt: 291, E(): 2.9e-14, (45.6% identity in 103 aa overlap), similar to MTCY339.37c, (35.0% identity in 100 aa overlap). P9WLF5 No cell wall and cell processes P64969 P9WLF5 GO:0005886,GO:0016021 ML1789 MMAR_3368 MSMEG_1231 Mb2295 NC_000962.3 Mycobrowser_v4 CDS 737268 738149 . - 0 Rv0643c mmaA3 Involved in mycolic acids modification. Catalyzes unusual S-adenosyl-methionine-dependent transformation of a cis-olefin mycolic acid into a secondary alcohol. Catalyzes introduction of a hydroxyl group at the distal position on mycolic acid chains to produce the hydroxyl mycolate. Mycolic acids represent a major constituent of the mycobacterial cell wall complex. Methyl transfer results in formation of a secondary hydroxy group with an adjacent methyl branch; olefinic mycolic acid methyl transferase. Methoxy mycolic acid synthase 3 MmaA3 (methyl mycolic acid synthase 3) (MMA3) (hydroxy mycolic acid synthase) Rv0643c, (MTCY20H10.24c), len: 293 aa. MmaA3, methoxy mycolic acid synthase 3 (methyltransferase) (see citations below). Equivalent to AAC44875|AAC44875.1|cmaB methyl transferase (mycolic acid modification protein) from Mycobacterium bovis BCG strain Pasteur (289 aa); and highly similar to others from Mycobacteria e.g. upstream ORF P72028|mmaA4|Rv0642c|MTCY20H10.23c putative methoxy mycolic acid synthase 4 from Mycobacterium tuberculosis (301 aa). P0CH91 No lipid metabolism P72027 P0CH91 GO:0008610,GO:0008825 2.1.1.- P0CH91 ML1902 Mb0662c NC_000962.3 Mycobrowser_v4 CDS 738297 739160 . - 0 Rv0644c mmaA2 Involved in mycolic acids modification. Catalyzes unusual S-adenosyl-methionine-dependent transformation of a cis-olefin mycolic acid into a secondary alcohol. Catalyzes introduction of a hydroxyl group at the distal position on mycolic acid chains to produce the hydroxyl mycolate. Mycolic acids represent a major constituent of the mycobacterial cell wall complex. Methyl transfer results in formation of a secondary hydroxy group with an adjacent methyl branch; olefinic mycolic acid methyl transferase. Has also cyclopropane function. Methoxy mycolic acid synthase 2 MmaA2 (methyl mycolic acid synthase 2) (MMA2) (hydroxy mycolic acid synthase) Rv0644c, (MTCY20H10.25c), len: 287 aa. MmaA2, methoxy mycolic acid synthase 2 (methyltransferase) (see citations below). Equivalent to AAC44874|AAC44874.1|cmaC methyl transferase (mycolic acid modification protein) from Mycobacterium bovis BCG strain Pasteur (287 aa); and highly similar to others from Mycobacteria e.g. upstream ORF P72028|mmaA4|Rv0642c|MTCY20H10.23c putative methoxy mycolic acid synthase 4 from Mycobacterium tuberculosis (301 aa). Note that alternative start is at position 739247. Q79FX6 No lipid metabolism 1TPY Q79FX6 Q79FX6 GO:0008610,GO:0008825 2.1.1.- Q79FX6 ML1901 Mb0663c NC_000962.3 Mycobrowser_v4 CDS 741151 742617 . - 0 Rv0647c Rv0647c Function unknown Conserved protein Rv0647c, (MTCY20H10.28c), len: 488 aa. Conserved protein, equivalent to NP_302277.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (448 aa). Also showing similarity to a variety of hypothetical ABC1-like proteins or conserved hypothetical proteins e.g. D90908_28|P73627 ABC1-like protein from Synechocystis (585 aa), FASTA scores: E(): 1.8e-31, (29.1% identity in 474 aa overlap); Q55884 HYPOTHETICAL6 5.0 KD protein (567 aa), FASTA scores: opt: 583, E(): 5.7e-30, (28.1% identity in 416 aa overlap); etc. Also similar to Rv3197 conserved hypothetical protein from Mycobacterium tuberculosis. P9WQI1 No conserved hypotheticals P96936 P9WQI1 GO:0006468,GO:0004672,GO:0005524 P9WQI1 ML1898 MMAR_0982 MSMEG_1353 Mb0666c NC_000962.3 Mycobrowser_v4 CDS 754980 755225 . - 0 Rv0660c mazE2 Function unknown Possible antitoxin MazE2 Rv0660c, (MTCI376.16), len: 81 aa. Possible mazE2, antitoxin, part of toxin-antitoxin (TA) operon with Rv0659c (See Pandey and Gerdes, 2005; Zhu et al., 2006), showing some similarity to AF016485_130 from Halobacterium sp (100 aa), FASTA scores: (32.4% identity in 74 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06779 No virulence, detoxification, adaptation O06779 O06779 Mb0679c NC_000962.3 Mycobrowser_v4 CDS 800487 800792 . + 0 Rv0700 rpsJ This protein is involved in the binding of tRNA to the ribosomes, and in the regulation of rRNA biosynthesis (by modulating the efficiency of transcriptional termination). Interacts with NUSB|Rv2533c. 30S ribosomal protein S10 RpsJ (transcription antitermination factor NusE) Rv0700, (MTCY210.19), len: 101 aa. rpsJ (alternate gene name: nusE), 30S ribosomal protein S10 (see Gopal et al., 2001), equivalent to RS10_MYCLE P307653 30S ribosomal protein S10 from Mycobacterium leprae (101 aa), FASTA scores: opt: 645, E(): 0, (97.0% identity in 101 aa overlap). Also highly similar to others e.g. CAB82069.1|AL161803 30S ribosomal protein S10 from Streptomyces coelicolor (102 aa); etc. Contains PS00361 Ribosomal protein S10 signature. Belongs to the S10P family of ribosomal proteins. P9WH67 No information pathways P0A5X0 P9WH67 GO:0003735,GO:0000049,GO:0006412,GO:0015935 P9WH67 ML1864,ML1864c MMAR_1030 MSMEG_1435 Mb0720 NC_000962.3 Mycobrowser_v4 CDS 740234 741139 . - 0 Rv0646c lipG Function unknown; lipolytic enzyme involved in cellular metabolism. Probable lipase/esterase LipG Rv0646c, (MTCY20H10.27c), len: 301 aa. Probable lipG, lipase/esterase, equivalent to NP_302278.1|NC_002677 probable hydrolase from Mycobacterium leprae (304 aa). Also highly similar to various hydrolases, especially lipases e.g. AA61351.1|X88895 carboxyl esterase from Acinetobacter calcoaceticus (312 aa), FASTA scores: opt: 867, E(): 0, (50.2% identity in 279 aa overlap); etc. Also similar to transferases e.g. P77026 macrolide 2'-phosphotransferase II from Escherichia coli (279 aa), FASTA scores: E(): 1.3e-14, (32.5% identity in 286 aa overlap). Similar to M. tuberculosis non-heme bromoperoxidases and epoxide hydrolases. P96935 No intermediary metabolism and respiration P96935 P96935 GO:0016787 3.1.-.- P96935 ML1899 MMAR_0981 MSMEG_1352 Mb0665c NC_000962.3 Mycobrowser_v4 CDS 747037 747945 . + 0 Rv0650 Rv0650 Function unknown; probably involved in specific sugar metabolism or regulation. Possible sugar kinase Rv0650, (MTCY20H10.31), len: 302 aa. Possible sugar kinase, highly similar to others e.g. CAB95296.1|AL359779 putative sugar kinase from Streptomyces coelicolor (317 aa); NP_406512.1|NC_003143 putative sugar kinase from Yersinia pestis (290 aa); NP_229269.1|NC_000853 glucokinase from Thermotoga maritima (317 aa); etc.Contains PS01125 ROK family signature. Belongs to the ROK (NAGC/XYLR) family. I6Y8D3 No intermediary metabolism and respiration P96939 I6Y8D3 GO:0016301 2.7.-.- I6Y8D3 ML1897,ML1897c MMAR_0987 MSMEG_1363 Mb0669 NC_000962.3 Mycobrowser_v4 CDS 748276 748812 . + 0 Rv0651 rplJ Involved in translation mechanisms. 50S ribosomal protein L10 RplJ Rv0651, (MTCY20H10.32), len: 178 aa. rplJ, 50S ribosomal protein L10, equivalent to NP_302276.1|NC_002677 50S ribosomal protein L10 from Mycobacterium leprae (177 aa). Also highly similar to others e.g. P36257|RL10_STRGR 50s ribosomal protein L10 from Streptomyces griseus (185 aa), FASTA scores: opt: 633, E(): 0, (59.0 % identity in 173 aa overlap); etc. Belongs to the L10P family of ribosomal proteins. P9WHE7 No information pathways P66044 P9WHE7 GO:0042254,GO:0003735,GO:0006412,GO:0005840 P9WHE7 ML1896,ML1896c MMAR_0990 MSMEG_1364 Mb0670 NC_000962.3 Mycobrowser_v4 CDS 791658 791846 . + 0 Rv0691A Rv0691A Electron carrier Mycofactocin precursor protein Rv0691A, len: 62 aa. Mycofactocin precursor protein. P9WJ81 No intermediary metabolism and respiration P9WJ81 NC_000962.3 Mycobrowser_v4 CDS 809946 810887 . + 0 Rv0713 Rv0713 Unknown Probable conserved transmembrane protein Rv0713, (MTCY210.32), len: 313 aa. Probable conserved transmembrane protein, similar to Rv3435c|MTCY77_7|O06252 from Mycobacterium tuberculosis (284 aa), FASTA scores: opt: 557, E(): 2.1e-29, (35.8% identity in 282 aa overlap); MLCB2492_12|O32991 hypothetical 10.7 kDa protein from Mycobacterium leprae (95 aa). I6WZ58 No cell wall and cell processes I6WZ58 GO:0016021 I6WZ58 ML1852,ML1852c MMAR_1044 Mb0734 NC_000962.3 Mycobrowser_v4 CDS 1662381 1662572 . + 0 Rv1473A Rv1473A Possibly involved in transcriptional mechanism Possible transcriptional regulatory protein Rv1473A, len: 63 aa. Possible transcriptional regulator, CDS predicted by GC plot. Similar to SCI8.24c|AL132644_24 putative transcriptional regulator from Streptomyces coelicolor (73 aa), FASTA scores: opt: 210, E(): 1.5e-08, (56.15% identity in 57 aa overlap). L7N691 No regulatory proteins L7N691 MMAR_2280 MSMEG_3141 Mb1509 NC_000962.3 Mycobrowser_v4 CDS 748849 749241 . + 0 Rv0652 rplL Involved in translation mechanisms: seems to be the binding site for several of the factors involved in protein synthesis and appears to be essential for accurate translation. 50S ribosomal protein L7/L12 RplL (SA1) Rv0652, (MTCY20H10.33), len: 130 aa. rplL (alternate gene name: L7|L12), 50S ribosomal protein L7/L12, equivalent to NP_302275.1|NC_002677 50S ribosomal protein L7/L12 from Mycobacterium leprae (130 aa); and P37381|RL7_MYCBO 50s ribosomal protein L7/L12 from Mycobacterium bovis (130 aa). Also highly similar to others e.g. P02396|RL7_STRGR 50S ribosomal protein L7/L12 from Streptomyces griseus (127 aa); etc. Belongs to the L12P family of ribosomal proteins. P9WHE3 No information pathways P0A5V2 P9WHE3 GO:0003735,GO:0006412,GO:0005840 P9WHE3 ML1895,ML1895c MMAR_0991 MSMEG_1365 Mb0671 NC_000962.3 Mycobrowser_v4 CDS 751517 752596 . + 0 Rv0655 mkl Thought to be involved in active transport of ribonucleotide across the membrane. Responsible for energy coupling to the transport system. Possible ribonucleotide-transport ATP-binding protein ABC transporter Mkl Rv0655, (MTCI376.21), len: 359 aa. Possible mkl, ribonucleotide-transport ATP-binding protein ABC transporter (see Braibant et al., 2000), equivalent to P30769|MKL_MYCLE|ML1892 possible ribonucleotide transport ATP-binding protein from Mycobacterium leprae (347 aa), FASTA scores: opt: 2021, E(): 0, (92.2% identity in 335 aa overlap). Also highly similar to many e.g. AB92896.1|AL356992 putative ABC-transporter ATP-binding protein from Streptomyces coelicolor (343 aa); NP_253146.1|NC_002516 probable ATP-binding component of ABC transporter from Pseudomonas aeruginosa (269 aa); P45393|YRBF_ECOLI hypothetical ABC transporter ATP-binding protein from Escherichia coli (269 aa), FASTA scores: opt: 644, E(): 3.4e-33, (38.5% identity in 244 aa overlap); etc. Also similar to many other Mycobacterium tuberculosis ABC transporters e.g. P71747|CYSA|Rv2397c|MTCY253.24 (351 aa), FASTA score: (33.6% identity in 241 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). P9WQL5 No cell wall and cell processes P63357 P9WQL5 GO:0016887,GO:0006810,GO:0005524 P9WQL5 ML1892,ML1892c MMAR_0994 MSMEG_1366 Mb0674 NC_000962.3 Mycobrowser_v4 CDS 752984 753367 . - 0 Rv0656c vapC6 Unknown Possible toxin VapC6 Rv0656c, (MTCI376.20), len: 127 aa. Possible vapC6, toxin, part of toxin-antitoxin (TA) operon with Rv0657c, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to other proteins from Mycobacterium tuberculosis e.g. Rv2757c, Rv2546, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WFB5 No virulence, detoxification, adaptation O06783 P9WFB5 P9WFB5 Mb0675c NC_000962.3 Mycobrowser_v4 CDS 753462 753617 . - 0 Rv0657c vapB6 Unknown Possible antitoxin VapB6 Rv0657c, (MTCI376.19), len: 51 aa. Possible vapB6, antitoxin, part of toxin-antitoxin (TA) operon with Rv0656c (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similarity with others from Mycobacterium tuberculosis e.g. Rv2009|MT2064.1|MTCY39.08c|YW08_MYCTU|Q10848 (80 aa), FASTA scores: opt: 107, E(): 0.0038, (45.8% identity in 48 aa overlap), Rv2871, Rv1560, etc. Also some similarity with AL020958|SC4H8_7 from Streptomyces coelicolor (66 aa), FASTA score: (41.0% identity in 39 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WJ57 No virulence, detoxification, adaptation O06782 P9WJ57 Mb0676c NC_000962.3 Mycobrowser_v4 CDS 798833 799444 . + 0 Rv0698 Rv0698 Function unknown Conserved hypothetical protein Rv0698, (MTCY210.15), len: 203 aa. Conserved hypothetical protein, highly similar to C-terminus of Rv3639c|MTY15C10.12 conserved hypothetical protein from Mycobacterium tuberculosis (188 aa), FASTA scores: E(): 2.1e-07, (54.8% identity in 73 aa overlap). P95044 No conserved hypotheticals P95044 Mb0718,Mb0717 NC_000962.3 Mycobrowser_v4 CDS 881075 881464 . - 0 Rv0786c Rv0786c Function unknown Conserved protein Rv0786c, (MTCY369.30c), len: 129 aa. Conserved protein, similar to three other hypothetical proteins from Streptomyces coelicolor e.g. SC7H1.08c|T35703 hypothetical protein (202 aa), FASTA scores: opt: 241, E(): 5.1e-10, (41.0% identity in 105 aa overlap); SC3A7.08|T29426 (211 aa). P71839 No conserved hypotheticals P71839 P71839 ML2221 MSMEG_5834 Mb0809c NC_000962.3 Mycobrowser_v4 CDS 755769 756023 . - 0 Rv0662c vapB7 Unknown Possible antitoxin VapB7 Rv0662c, (MTCI376.14), len: 84 aa. Possible vapB7, antitoxin, part of toxin-antitoxin (TA) operon with Rv0661c (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similarity with others from Mycobacterium tuberculosis e.g. Rv2871, Rv1241, Rv2550c, etc. Start changed since first submission, now 38 aa shorter. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06777 No virulence, detoxification, adaptation O06777 Mb0681c NC_000962.3 Mycobrowser_v4 CDS 759136 759309 . + 0 Rv0666 Rv0666 Unknown Possible membrane protein Rv0666, (MTCI376.10c), len: 57 aa. Possible membrane protein; has hydrophobic stretch at aa 29-47. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06773 No cell wall and cell processes O06773 Mb0685 NC_000962.3 Mycobrowser_v4 CDS 755335 755772 . - 0 Rv0661c vapC7 Unknown Possible toxin VapC7 Rv0661c, (MTCI376.15), len: 145 aa. Possible vapC7, toxin, part of toxin-antitoxin (TA) operon with Rv0662c, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. Rv2863|MTV003.09|MTV003_7 (126 aa), FASTA scores: E(): 0.00087, (30.4% identity in 125 aa overlap), Rv0749|MTV041.23 (163 aa); Rv0277c, Rv2530c, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WFB3 No virulence, detoxification, adaptation O06778 P9WFB3 P9WFB3 Mb0680c NC_000962.3 Mycobrowser_v4 CDS 758532 758804 . + 0 Rv0664 vapB8 Unknown Possible antitoxin VapB8 Rv0664, (MTCI376.12c), len: 90 aa. Possible vapB8, antitoxin, part of toxin-antitoxin (TA) operon with Rv0665 (See Arcus et al., 2005; Pandey and Gerdes, 2005). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06775 No virulence, detoxification, adaptation O06775 O06775 Mb0683 NC_000962.3 Mycobrowser_v4 CDS 758801 759139 . + 0 Rv0665 vapC8 Unknown Possible toxin VapC8 Rv0665, (MTCI376.11c), len: 112 aa. Possible vapC8, toxin, part of toxin-antitoxin (TA) operon with Rv0664, contains PIN domain (See Arcus et al. 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. Rv0627 (135 aa), and Rv0595c. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WFB1 No virulence, detoxification, adaptation O06774 P9WFB1 P9WFB1 Mb0684 NC_000962.3 Mycobrowser_v4 CDS 759807 763325 . + 0 Rv0667 rpoB Catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [catalytic activity: N nucleoside triphosphate = N diphosphate + {RNA}(N)]. DNA-directed RNA polymerase (beta chain) RpoB (transcriptase beta chain) (RNA polymerase beta subunit) Rv0667, (MTCI376.08c), len: 1172 aa. RpoB, DNA-directed RNA polymerase, beta chain (see Miller et al., 1994; Ahmad et al., 2000), equivalent to P30760|RPOB_MYCLE|ML1891 DNA-directed RNA polymerase beta chain from Mycobacterium leprae (1178 aa). Also highly similar to others e.g. AAF60349.1|AF242549_1|AF242549 DNA-dependent RNA polymerase beta subunit from Amycolatopsis mediterranei (1167 aa); CAB77428.1|AL160431 DNA-directed RNA polymerase beta chain from Streptomyces coelicolor (1161 aa); etc. Start site chosen on basis of RBS but alternative start exists at position 14359. Belongs to the RNA polymerase beta chain family. P9WGY9 No information pathways P0A680 P9WGY9 GO:0003899,GO:0046677,GO:0032549,GO:0006351,GO:0003677 2.7.7.6 RIF P9WGY9 ML1891,ML1891c MMAR_0995 MSMEG_1367 Mb0686 NC_000962.3 Mycobrowser_v4 CDS 791831 792160 . + 0 Rv0692 Rv0692 Function unknown Conserved hypothetical protein Rv0692, (MTCY210.09), len: 109 aa. Conserved hypothetical protein, highly similar to U17129|RSU17129_3|AAC77477.1 unknown protein from Rhodococcus erythropolis (95 aa), FASTA scores: opt: 393, E(): 8.8e-22, (68.2% identity in 88 aa overlap). P95038 No conserved hypotheticals P95038 ML1871,ML1871c MMAR_1020 MSMEG_1422 Mb0711 NC_000962.3 Mycobrowser_v4 CDS 799629 799850 . + 0 Rv0699 Rv0699 Unknown Hypothetical protein Rv0699, (MTCY210.17), len: 73 aa. Hypothetical unknown protein. P95046 No conserved hypotheticals P95046 P95046 Mb0719 NC_000962.3 Mycobrowser_v4 CDS 769792 770550 . + 0 Rv0670 end Involved in base excision repair. Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5' ends that are base-free deoxyribose 5-phosphate residues [catalytic activity: endonucleolytic cleavage to 5'-phosphooligonucleotide end-products]. Probable endonuclease IV End (endodeoxyribonuclease IV) (apurinase) Rv0670, (MTCI376.04c), len: 252 aa. Probable end (alternate gene name: nfo), endonuclease IV (apurinase) (see citation below), equivalent to END_MYCLE|P30770|NFO|ML1889 probable endonuclease IV (apurinase) from Mycobacterium leprae (252 aa), FASTA scores: opt: 1463, E(): 0, (85.6% identity in 250 aa overlap). Also similar to others e.g. Q9S2N2|END4_STRCO|NFO|SC6E10.05 probable endonuclease IV from Streptomyces coelicolor (294 aa); etc. Contains PS00729 AP endonucleases family 2 signatures 1 and 2 (PS00729, and PS00730). Belongs to the AP endonucleases family 2. Cofactor: binds 3 zinc ions. The transcription of this CDS seems negatively regulated by the product of mce2R|Rv0586 (See Santangelo et al., 2009). P9WQ13 No information pathways P63535 P9WQ13 GO:0006281,GO:0008833,GO:0005622,GO:0008270,GO:0003677 3.1.21.2 P9WQ13 ML1889,ML1889c MMAR_0999 MSMEG_1383 Mb0689 NC_000962.3 Mycobrowser_v4 CDS 778477 778905 . - 0 Rv0677c mmpS5 Unknown Possible conserved membrane protein MmpS5 Rv0677c, (MTV040.05c), len: 142 aa. Possible mmpS5, conserved membrane protein (see Tekaia et al., 1999), highly similar to other Mycobacterial proteins e.g. P54880|MMS4_MYCLE putative membrane protein from Mycobacterium leprae (154 aa), FASTA scores: opt: 443, E(): 1.4e-23, (47.1% identity in 155 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis. Belongs to the MmpS family. Predicted to be an outer membrane protein (See Song et al., 2008). P9WJS7 No cell wall and cell processes P65380 P9WJS7 GO:0016021 P9WJS7 MMAR_1006 MSMEG_0226,MSMEG_3495 Mb0696c NC_000962.3 Mycobrowser_v4 CDS 811373 811741 . + 0 Rv0714 rplN This protein binds directly to 23S ribosomal RNA. 50S ribosomal protein L14 RplN Rv0714, (MTCY210.33), len: 122 aa. rplN, 50S ribosomal protein L14, equivalent to O32993|MLCB2492_14|ML1849|RL14_MYCLE 50S ribosomal protein L14 from Mycobacterium leprae (122 aa). Also highly similar to others e.g. CAB82080.1|AL161803 50S ribosomal protein L14 from Streptomyces coelicolor (122 aa); P33100|RL14_MICLU 50s ribosomal protein L14 from Micrococcus luteus (122 aa), FASTA scores: opt: 674, E(): 0, (85.2% identity in 122 aa overlap); etc. Contains PS00049 Ribosomal protein L14 signature. Belongs to the L14P family of ribosomal proteins. P9WHD9 No information pathways P66069 P9WHD9 GO:0019843,GO:0003735,GO:0006412,GO:0015934 P9WHD9 ML1849,ML1849c MMAR_1045 MSMEG_1465 Mb0735 NC_000962.3 Mycobrowser_v4 CDS 2348558 2349292 . - 0 Rv2091c Rv2091c Unknown Probable membrane protein Rv2091c, (MTCY49.31c), len: 244 aa. Probable membrane protein; contains potential transmembrane region. Repetitive ORF. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WLJ5 No cell wall and cell processes P9WLJ5 GO:0016021 P9WLJ5 ML1334 MMAR_3077 MSMEG_3884 Mb2118c NC_000962.3 Mycobrowser_v4 CDS 773123 774061 . + 0 Rv0673 echA4 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Possible enoyl-CoA hydratase EchA4 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv0673, (MTCI376.01c, MTV040.01), len: 312 aa. Possible echA4, enoyl-CoA hydratase, showing similarity with others e.g. NP_419216.1|NC_002696 enoyl-CoA hydratase/isomerase family protein from Caulobacter crescentus (256 aa); Q52995|ECHH_RHIME probable enoyl-CoA hydratase from Sinorhizobium meliloti (257 aa), FASTA scores: opt: 210, E(): 1.2e-06, (27.9% identity in 280 aa overlap); etc. Also similar to other enoyl-CoA hydratases from Mycobacterium tuberculosis e.g. P95279|MTCY09F9.29|ECHA13|Rv1935c|MTCY09F9.29 enoyl-CoA hydratase (318 aa), FASTA score: (27.1% identity in 280 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). I6Y8F2 No lipid metabolism O86369 I6Y8F2 GO:0016853,GO:0008152,GO:0004300 4.2.1.17 I6Y8F2 ML1887,ML1887c MMAR_1002 MSMEG_1388 Mb0692 NC_000962.3 Mycobrowser_v4 CDS 774064 774786 . + 0 Rv0674 Rv0674 Function unknown Conserved hypothetical protein Rv0674, (MTV040.02), len: 240 aa. Conserved hypothetical protein, highly similar to AC13063.1|AL445503 conserved hypothetical protein from Streptomyces coelicolor (268 aa); and similar to NP_438100.1|NC_003078 putative regulator of phenylacetic acid degradation ArsR family protein from Sinorhizobium meliloti (306 aa) and other proteins e.g. AB011837|AB011837_13 hypothetical protein from Bacillus halodurans (298 aa), FASTA scores: opt: 148, E(): 0.0081, (25.1% identity in 235 aa overlap); etc. I6WZ26 No conserved hypotheticals 3KFW O53782 I6WZ26 I6WZ26 ML1886,ML1886c MMAR_1003 MSMEG_1389 Mb0693 NC_000962.3 Mycobrowser_v4 CDS 775586 778480 . - 0 Rv0676c mmpL5 Unknown. Thought to be involved in fatty acid transport. Probable conserved transmembrane transport protein MmpL5 Rv0676c, (MTV040.04c), len: 964 aa. Probable mmpL5, conserved transmembrane transport protein (see Tekaia et al., 1999), member of RND superfamily, highly similar to other Mycobacterial proteins e.g. MTV037_14, MTCY98_8, MTCY20G9_34, MTCY4D9_15, MTCY48_8, MTCY19G5_6, MTV005_19, etc. Also similar to other Mycobacterial mmpl proteins e.g. P54881|MML4_MYCLE putative membrane protein MMPL4 from Mycobacterium leprae (959 aa), FASTA scores: opt: 3991, E(): 0, (62.8% identity in 933 aa overlap); etc. Belongs to the MmpL family. P9WJV1 No cell wall and cell processes O53784 P9WJV1 GO:0005886,GO:0016021 MMAR_1005 MSMEG_0225,MSMEG_3496 Mb0695c NC_000962.3 Mycobrowser_v4 CDS 780042 780416 . - 0 Rv0680c Rv0680c Unknown Probable conserved transmembrane protein Rv0680c, (MTV040.08c), len: 124 aa. Possible conserved transmembrane protein, showing similarity with C-terminal part of Rv0314c|Z96800|MTCY63.19c conserved hypothetical protein from Mycobacterium tuberculosis (220 aa), FASTA scores: opt: 175, E(): 2.2e-05, (31.4% identity in 102 aa overlap). Also some similarity to upstream ORF Rv0679c|MTV040.07c conserved hypothetical threonine rich protein (124 aa), FASTA score: (35.1% identity in 131 aa overlap). Contains possible membrane spanning regions. I6Y4F1 No cell wall and cell processes O53788 I6Y4F1 GO:0016021 ML1883 MMAR_1009 MSMEG_1394 Mb0699c NC_000962.3 Mycobrowser_v4 CDS 780721 781311 . + 0 Rv0681 Rv0681 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (possibly TetR-family) Rv0681, (MTV040.09), len: 196 aa. Probable transcription regulator, TetR family, similar to others and especially many tetracycline repressors e.g. T34657 probable transcription regulator from Streptomyces coelicolor (189 aa); AF0278|AF027868_40|NP_389788.1|NC_000964 yobS regulator from Bacillus subtilis (191 aa), FASTA scores: opt: 213, E(): 1.6e-07, (28.8% identity in 153 aa overlap); P09164|TER4_ECOLI tetracycline repressor protein from Escherichia coli (217 aa), FASTA scores: opt: 145, E(): 0.0068, (39.0% identity in 59 aa overlap); etc. Contains helix-turn-helix motif at aa 28-49 (Score 1020, +2.66 SD). O53789 No regulatory proteins O53789 O53789 GO:0006350,GO:0003700,GO:0006355 O53789 ML1882,ML1882c MMAR_1010 MSMEG_1397 Mb0700 NC_000962.3 Mycobrowser_v4 CDS 781934 782404 . + 0 Rv0683 rpsG Protein S7 binds specifically to part of the 3' end of 16S ribosomal RNA. 30S ribosomal protein S7 RpsG Rv0683, (MTV040.11), len: 156 aa. rpsG, 30S ribosomal protein S7 (see citation below), equivalent to others from Mycobacteria e.g. P41193|RS7_MYCSM 30S ribosomal protein S7 from Mycobacterium smegmatis (156 aa), FASTA scores: opt: 986, E(): 0, (96.2% identity in 156 aa overlap); Q53539|RS7_MYCBO 30S ribosomal protein S7 from Mycobacterium bovis (156 aa); etc. Also highly similar to others e.g. Q9L0K4|RS7_STRCO 30S ribosomal protein S7 from Streptomyces coelicolor (156 aa); etc. Contains PS00052 Ribosomal protein S7 signature. Belongs to the S7P family of ribosomal proteins. P9WH29 No information pathways P41194 P9WH29 GO:0015935,GO:0003735,GO:0000049,GO:0006412,GO:0019843 P9WH29 ML1879,ML1879c MMAR_1012 MSMEG_1399 Mb0702 NC_000962.3 Mycobrowser_v4 CDS 782485 784590 . + 0 Rv0684 fusA1 This protein promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. Probable elongation factor G FusA1 (EF-G) Rv0684, (MTV040.12, MTCY210.01), len: 701 aa. Probable fusA1, elongation factor G, equivalent to P30767|EFG_MYCLE|S31150 translation elongation factor EF-G from Mycobacterium leprae (701 aa), FASTA scores: opt: 2521, E(): 0, (88.2% identity in 432 aa overlap). Also highly similar to others e.g. CAB81852.1|AL161691 elongation factor G from Streptomyces coelicolor (708 aa); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS00301 GTP-binding elongation factors signature. Belongs to the GTP-binding elongation factor family, EF-G/EF-2 subfamily. Note that previously known as fusA. P9WNM7 No information pathways P0A556 P9WNM7 GO:0003924,GO:0005737,GO:0003746,GO:0006414,GO:0005525 P9WNM7 ML1878,ML1878c MMAR_1013 Mb0703 NC_000962.3 Mycobrowser_v4 CDS 784821 786011 . + 0 Rv0685 tuf This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Probable iron-regulated elongation factor TU Tuf (EF-TU) Rv0685, (MTCY210.02), len: 396 aa. Probable tuf, iron-regulated elongation factor EF-Tu, equivalent to JC2262 translation elongation factor Tu from Mycobacterium leprae (396 aa). Also highly similar to others e.g. P42439|EFTU_CORGL elongation factor TU (EF-TU) from Corynebacterium glutamicum (396 aa); etc. Contains PS00017 ATP/GTP-binding site motif A, and PS00301 GTP-binding elongation factors signature. Belongs to the GTP-binding elongation factor family, EF-TU/EF-1A subfamily. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WNN1 No information pathways P0A558 P9WNN1 GO:0003924,GO:0005737,GO:0003746,GO:0006414,GO:0005525 P9WNN1 ML1877,ML1877c MMAR_1014 MSMEG_1401 Mb0704 NC_000962.3 Mycobrowser_v4 CDS 790024 791073 . - 0 Rv0690c Rv0690c Function unknown Conserved hypothetical protein Rv0690c, (MTCY210.07c), len: 349 aa. Conserved hypothetical protein, showing similarity with NP_386956.1|NC_003047 conserved hypothetical protein from Sinorhizobium meliloti (358 aa); NP_356573.1|NC_003063 AGR_L_1570p from Agrobacterium tumefaciens (346 aa); NP_421938.1|NC_002696 conserved hypothetical protein from Caulobacter crescentus (370 aa). I6Y4G1 No conserved hypotheticals P95036 I6Y4G1 I6Y4G1 ML1873 MMAR_1018 Mb0709c NC_000962.3 Mycobrowser_v4 CDS 792157 793332 . + 0 Rv0693 pqqE Required for coenzyme pyrrolo-quinoline-quinone (PQQ) biosynthesis. Probable coenzyme PQQ synthesis protein E PqqE (coenzyme PQQ synthesis protein III) Rv0693, (MTCY210.10), len: 391 aa. Probable pqqE (alternate gene name: pqqIII), coenzyme PQQ synthesis protein E, similar to others AE001109_9|O30258|PQQE coenzyme PQQ synthesis protein from Archaeoglobus fulgidus (375 aa), FASTA scores: E(): 1.6e-16, (28.1% identity in 377 aa overlap); PQQE_ACICA|P07782 coenzyme pqq synthesis protein e from Acinetobacter calcoaceticus (384 aa), FASTA scores: opt: 302, E(): 1.8e-12, (23.9% identity in 377 aa overlap); etc. Also similar to C-terminus of heme biosynthesis proteins e.g. O28270|AF2009 heme biosynthesis protein (NIRJ-2) from Archaeoglobus fulgidus (468 aa). Note that also highly similar to U17129|RSU17129_4|AAC77478.1 unknown protein from Rhodococcus erythropolis (405 aa), FASTA scores: opt: 1997, E(): 0, (73.3% identity in 390 aa overlap). Could belong to the MoaA / NifB / PqqE family. P9WJ79 No intermediary metabolism and respiration P95039 P9WJ79 GO:0051536,GO:0003824 P9WJ79 ML1870,ML1870c MMAR_1021 MSMEG_1423 Mb0712 NC_000962.3 Mycobrowser_v4 CDS 794715 795470 . + 0 Rv0695 Rv0695 Function unknown Conserved hypothetical protein Rv0695, (MTCY210.12), len: 251 aa. Conserved hypothetical protein, similar to many creatinine amidohydrolases or hypothetical proteins e.g. NP_443048.1|NC_000911 creatinine amidohydrolase from Synechococcus sp. PCC 6803 (273 aa); NP_466169.1|NC_003210 protein similar to creatinine amidohydrolase from Listeria monocytogenes (249 aa); T35153|SC5A7.04c hypothetical protein from Streptomyces coelicolor (273 aa); etc. Note that highly similar to RSU17129_10|AAC77474.1|U17129 unknown protein from Rhodococcus erythropolis (230 aa), FASTA scores: opt: 693, E(): 0, (55.7% identity in 237 aa overlap). P9WP59 No conserved hypotheticals P95041 P9WP59 P9WP59 ML1868,ML1868c MMAR_1023 MSMEG_1425 Mb0714 NC_000962.3 Mycobrowser_v4 CDS 796933 798372 . + 0 Rv0697 Rv0697 Function unknown; probably involved in cellular metabolism. Probable dehydrogenase Rv0697, (MTCY210.14, unknown), len: 479 aa. Probable dehydrogenase, highly similar to P30772|YTUR_MYCLE hypothetical 24 kDa protein from Mycobacterium leprae (220 aa), FASTA scores: opt: 557, E(): 1.7e-28, (46.2% identity in 223 aa overlap). Also highly similar to P46371|YTH2_RHOER hypothetical 53.0 KDA GMC-type oxidoreductase from Rhodococcus erythropolis (493 aa); and similar to many dehydrogenases e.g. NP_250814.1|NC_002516 probable dehydrogenase from Pseudomonas aeruginosa (545 aa); BAA13145.1|D86622 FAD dependent L-sorbose dehydrogenase from Gluconobacter oxydans (531 aa); etc. Also similar to Rv1279 conserved hypothetical protein from Mycobacterium tuberculosis. I6Y8H4 No intermediary metabolism and respiration P95043 I6Y8H4 GO:0006066,GO:0055114,GO:0016614,GO:0050660 1.-.-.- I6Y8H4 ML1866,ML1866c MMAR_1025 MSMEG_1428 Mb0716 NC_000962.3 Mycobrowser_v4 CDS 800809 801462 . + 0 Rv0701 rplC This protein binds directly to 23S ribosomal RNA and may participate in the formation of the peptidyltransferase center of the ribosome. 50S ribosomal protein L3 RplC Rv0701, (MTCY210.20), len: 217 aa. rplC, 50S ribosomal protein L3, equivalent to O06044|RL3_MYCBO 50S ribosomal protein L3 from Mycobacterium bovis BCG (217 aa); and P30762|RL3_MYCLE 50S ribosomal protein L3 from Mycobacterium leprae (217 aa). Also highly similar to others e.g. CAB82070.1|AL161803 50S ribosomal protein L3 from Streptomyces coelicolor (214 aa); P52860|RL3_THETH ribosomal protein l3 from Thermus aquaticus (206 aa), FASTA scores: opt: 717, E(): 0, (55.2% identity in 210 aa overlap); etc. Contains PS00474 Ribosomal protein L3 signature. Belongs to the L3P family of ribosomal proteins. P9WH87 No information pathways P60442 P9WH87 GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WH87 ML1863,ML1863c MMAR_1031 MSMEG_1436 Mb0721 NC_000962.3 Mycobrowser_v4 CDS 801462 802133 . + 0 Rv0702 rplD This protein binds directly and specifically to 23S rRNA. 50S ribosomal protein L4 RplD Rv0702, (MTCY210.21), len: 223 aa. rplD, 50S ribosomal protein L4, equivalent to O06045|RL4_MYCBO 50S ribosomal protein L4 from Mycobacterium bovis BCG (223 aa); O06114|RL4_MYCSM 50S ribosomal protein L4 from Mycobacterium smegmatis (215 aa); and MLCB2492_3 50S ribosomal protein L4 from Mycobacterium leprae (230 aa). Also highly similar to others e.g. CAB82071.1|AL161803 50S ribosomal protein L4 from Streptomyces coelicolor (219 aa); P28601|RL4_BACST 50s ribosomal protein L4 from Bacillus stearothermophilus (207 aa), FASTA scores: opt: 522, E(): 3.5e-26, (42.4% identity in 198 aa overlap); etc. Belongs to the L4P family of ribosomal proteins. P9WH85 No information pathways P60729 P9WH85 GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WH85 ML1862,ML1862c MMAR_1032 MSMEG_1437 Mb0722 NC_000962.3 Mycobrowser_v4 CDS 802133 802435 . + 0 Rv0703 rplW Binds to a specific region on the 23S rRNA. 50S ribosomal protein L23 RplW Rv0703, (MTCY21.22), len: 100 aa. rplW, 50S ribosomal protein L23, equivalent to O06046|RL23_MYCBO 50S ribosomal protein L23 from Mycobacterium bovis BCG (100 aa); and MLCB2492_4 50S ribosomal protein L23 from Mycobacterium leprae (100 aa). Also highly similar to others e.g. CAB82072.1|AL161803 50S ribosomal protein L23 from Streptomyces coelicolor (139 aa) (N-terminus longer); P04454|RL23_BACST 50s ribosomal protein L23 from Bacillus stearothermophilus (95 aa), FASTA scores: opt: 275, E(): 1.4e-13, (50.5% identity in 95 aa overlap); etc. Contains PS00050 Ribosomal protein L23 signature. Belongs to the L23P family of ribosomal proteins. P9WHB9 No information pathways P95051 P9WHB9 GO:0019843,GO:0005840,GO:0003735,GO:0006412,GO:0000166 P9WHB9 ML1861,ML1861c MMAR_1033 MSMEG_1438 Mb0723 NC_000962.3 Mycobrowser_v4 CDS 802528 803370 . + 0 Rv0704 rplB This protein is a primary 23S rRNA-binding protein. It has peptidyltransferase activity. 50S ribosomal protein L2 RplB Rv0704, (MTCY210.23), len: 280 aa. rplB, 50S ribosomal protein L2, equivalent to O06047|RL2_MYCBO 50S ribosomal protein L2 from Mycobacterium bovis BCG (280 aa); and MLCB2492_5M 50S ribosomal protein L2 from Mycobacterium leprae (280 aa). Also highly similar to others e.g. CAB82073.1|AL161803 50S ribosomal protein L2 from Streptomyces coelicolor (278 aa); P42919|RL2_BACSU 50s ribosomal protein l2 (bl2) from Bacillus subtilis (276 aa), FASTA scores: opt: 1179, E(): 0, (61.1% identity in 275 aa overlap); etc. Contains PS00467 Ribosomal protein L2 signature. Belongs to the L2P family of ribosomal proteins. P9WHA5 No information pathways P95052 P9WHA5 GO:0019843,GO:0003735,GO:0016740,GO:0006412,GO:0015934 P9WHA5 ML1860,ML1860c MMAR_1034 MSMEG_1439 Mb0724 NC_000962.3 Mycobrowser_v4 CDS 808746 809645 . + 0 Rv0712 Rv0712 Function unknown Conserved protein Rv0712, (MTCY210.31), len: 299 aa. Conserved protein, similar to others e.g. NP_106128.1|NC_002678 hypothetical protein from Mesorhizobium loti (372 aa); D90901_33|P72841 hypothetical 48.1 kDa protein from Synechocystis sp (410 aa), FASTA scores: E(): 1.1e-07, (28.8% identity in 299 aa overlap); etc. Slight similarity to carboxykinases. Similar to C-terminal part of Rv3703c conserved hypothetical protein from Mycobacterium tuberculosis (425 aa). I6Y8I5 No conserved hypotheticals P95060 I6Y8I5 I6Y8I5 ML1851,ML1851c MMAR_1043 MSMEG_1226,MSMEG_1452 Mb0733 NC_000962.3 Mycobrowser_v4 CDS 804282 805106 . + 0 Rv0707 rpsC This protein is involved in the binding of initiator met-tRNA. 30S ribosomal protein S3 RpsC Rv0707, (MTCY210.26), len: 274 aa. rpsC, 30S ribosomal protein S3, equivalent to O06048|RS3_MYCBO|MBS10OPER_8 30S ribosomal protein S3 from Mycobacterium bovis BCG (274 aa); and MLCB2492_8 30S ribosomal protein S3 from Mycobacterium leprae (281 aa). Also highly similar to others e.g. CAB82076.1|AL161803 30S ribosomal protein S3 from Streptomyces coelicolor (277 aa); P21465|RS3_BACSU 30s ribosomal protein s3 (bs3) (bs2) from Bacillus subtilis (217 aa), FASTA scores: opt: 794, E(): 0, (52.8% identity in 212 aa overlap); etc. Belongs to the S3P family of ribosomal proteins. P9WH37 No information pathways P0A5X6 P9WH37 GO:0015935,GO:0003735,GO:0006412,GO:0019843 P9WH37 ML1857,ML1857c MMAR_1037 MSMEG_1442 Mb0727 NC_000962.3 Mycobrowser_v4 CDS 805110 805526 . + 0 Rv0708 rplP This protein binds directly to 23S ribosomal RNA and is located at the a site of the peptidyltransferase center. 50S ribosomal protein L16 RplP Rv0708, (MTCY210.27), len: 138 aa. rplP, 50S ribosomal protein L16, equivalent to O06049|RL16_MYCBO|MBS10OPER_9 50S ribosomal protein L16 from Mycobacterium bovis BCG (138 aa); and MLCB2492_9 50S ribosomal protein L16 from Mycobacterium leprae (138 aa). Also highly similar to others e.g. CAB82077.1|AL161803 50S ribosomal protein L16 from Streptomyces coelicolor (139 aa); P14577|RL16_BACSU 50s ribosomal protein l16 from Bacillus subtilis (144 aa), FASTA scores: opt: 600, E(): 0, (63.2% identity in 136 aa overlap); etc. Contains PS00701 Ribosomal protein L16 signature 2. Belongs to the L16P family of ribosomal proteins. P9WHD5 No information pathways P95056 P9WHD5 GO:0005840,GO:0003735,GO:0000049,GO:0006412,GO:0019843 P9WHD5 ML1856,ML1856c MMAR_1038 MSMEG_1443 Mb0728 NC_000962.3 Mycobrowser_v4 CDS 805526 805759 . + 0 Rv0709 rpmC Involved in translation mechanisms. 50S ribosomal protein L29 RpmC Rv0709, (MTCY210.28), len: 77 aa. rpmC, 50S ribosomal protein L29, equivalent to O06050|RL29_MYCBO|MBS10OPER_10 50S ribosomal protein L29 from Mycobacterium bovis BCG (75 aa); and O32989|RL29_MYCLE|MLCB2492_10 50S ribosomal protein L29 from Mycobacterium leprae (80 aa). Also highly similar to others e.g. Q9L0D2|RL29_STRCO 50S ribosomal protein L29 from Streptomyces coelicolor (74 aa); P12873|RL29_BACSU 50s ribosomal protein l29 from Bacillus subtilis (66 aa), FASTA scores: opt: 225, E(): 8.3e-11, (58.6% identity in 58 aa overlap); etc. Belongs to the L29P family of ribosomal proteins. P9WHA7 No information pathways P95057 P9WHA7 GO:0003735,GO:0006412,GO:0005840 P9WHA7 ML1855,ML1855c MMAR_1039 MSMEG_1444 Mb0729 NC_000962.3 Mycobrowser_v4 CDS 805756 806166 . + 0 Rv0710 rpsQ Protein S17 binds specifically to the 5' end of 16S ribosomal RNA. 30S ribosomal protein S17 RpsQ Rv0710, (MTCY210.29), len: 136 aa. rpsQ, 30S ribosomal protein S17, equivalent to O06051|RS17_MYCBO 30S|MBS10OPER_11 30S ribosomal protein S17 from Mycobacterium bovis BCG (136 aa); and MLCB2492_11 30S ribosomal protein S17 from Mycobacterium leprae (126 aa). Also highly similar to others e.g. CAB82079.1|AL161803 30S ribosomal protein S17 from Streptomyces coelicolor (95 aa); P12874|RS17_BACSU 30s ribosomal protein s17 (bs 16) from Bacillus subtilis (86 aa), FASTA scores: opt: 305, E(): 1.6e-11, (60.5% identity in 81 aa overlap); etc. Contains PS00056 Ribosomal protein S17 signature. P9WH51 No information pathways P95058 P9WH51 GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WH51 ML1854,ML1854c MMAR_1040 MSMEG_1445 Mb0730 NC_000962.3 Mycobrowser_v4 CDS 850741 851466 . - 0 Rv0756c Rv0756c Unknown Unknown protein Rv0756c, (MTCY369.01c), len: 241 aa. Unknown protein. P71813 No conserved hypotheticals P71813 ML2240 MMAR_4943 MSMEG_5873 Mb0779c NC_000962.3 Mycobrowser_v4 CDS 1052360 1052638 . + 0 Rv0942 Rv0942 Unknown Hypothetical protein Rv0942, (MTCY10D7.32c), len: 92 aa. Hypothetical unknown protein. P9WKN9 No conserved hypotheticals P9WKN9 P9WKN9 Mb0967 NC_000962.3 Mycobrowser_v4 CDS 2249478 2250974 . - 0 Rv2004c Rv2004c Unknown Conserved protein Rv2004c, (MTCY39.13), len: 498 aa. Conserved protein. Contains PS00017 ATP/GTP-binding site motif A. P9WLN3 No conserved hypotheticals P0A5F9 P9WLN3 GO:0005618,GO:0001906,GO:0005576,GO:0005524 MMAR_3476 MSMEG_3942 Mb2027c NC_000962.3 Mycobrowser_v4 CDS 812627 812812 . + 0 Rv0717 rpsN1 Known to be required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the a site. 30S ribosomal protein S14 RpsN1 Rv0717, (MTCY210.36), len: 61 aa. rpsN1, 30S ribosomal protein S14, equivalent to MLCB2492_17|O32996 ribosomal protein S14 from Mycobacterium leprae (61 aa). Also highly similar to others e.g. CAB82083.1|AL161803 30S ribosomal protein S14 from Streptomyces coelicolor (61 aa); P24320|RS14_THETH 30s ribosomal protein S14 from Thermus aquaticus (subsp. thermophilus) (60 aa), FASTA scores: opt: 316, E(): 2e-19,(70.0% identity in 60 aa overlap); etc. Contains PS00527 Ribosomal protein S14 signature. Belongs to the S14P family of ribosomal proteins. Note that previously known as rpsN. P9WH57 No information pathways P0A5X2 P9WH57 GO:0019843,GO:0005840,GO:0003735,GO:0006412,GO:0046872 P9WH57 ML1846,ML1846c MMAR_1048 MSMEG_1468 Mb0738 NC_000962.3 Mycobrowser_v4 CDS 811742 812059 . + 0 Rv0715 rplX This protein is found in the ribonucleoprotein core and is involved in the early assembly of the 50S subunit. It is not involved in the functions of the mature 50S subunit. 50S ribosomal protein L24 RplX Rv0715, (MTCY210.34), len: 105 aa. rplX, 50S ribosomal protein L24, equivalent to O32994|MLCB2492_15 50S ribosomal protein L24 from Mycobacterium leprae (105 aa). Also highly similar to others e.g. CAB82081.1|AL161803 50S ribosomal protein L24 from Streptomyces coelicolor (107 aa); P12876|RL24_BACSU 50s ribosomal protein L24 (bl23) from Bacillus subtilis (103 aa), FASTA scores: opt: 363, E(): 1.8e-18, (56.7% identity in 104 aa overlap); etc. Contains PS01108 Ribosomal protein L24 signature. Belongs to the L24P family of ribosomal proteins. P9WHB7 No information pathways P60627 P9WHB7 GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WHB7 ML1848,ML1848c MMAR_1046 MSMEG_1466 Mb0736 NC_000962.3 Mycobrowser_v4 CDS 812059 812622 . + 0 Rv0716 rplE This is one of 3 proteins that mediate the attachment of the 5S RNA into the large ribosomal subunit. 50S ribosomal protein L5 RplE Rv0716, (MTCY210.35), len: 187 aa. rplE, 50S ribosomal protein L5, equivalent to MLCB2492_16 50S ribosomal protein L5 from Mycobacterium leprae (187 aa). Also highly similar to others e.g. CAB82082.1|AL161803 50S ribosomal protein L5 from Streptomyces coelicolor (185 aa); P33098|RL5_MICLU 50S ribosomal protein L5 from Micrococcus luteus (191 aa), FASTA scores: opt: 930, E(): 0, (73.8% identity in 183 aa overlap); etc. Belongs to the L5P family of ribosomal proteins. P9WH83 No information pathways P62403 P9WH83 GO:0005840,GO:0003735,GO:0000049,GO:0006412,GO:0019843 P9WH83 ML1847,ML1847c MMAR_1047 MSMEG_1467 Mb0737 NC_000962.3 Mycobrowser_v4 CDS 813398 813937 . + 0 Rv0719 rplF This protein binds directly to 23S ribosomal RNA and is located at the aminoacyl-tRNA binding site of the peptidyltransferase center. 50S ribosomal protein L6 RplF Rv0719, (MTCY210.38), len: 179 aa. rplF, 50S ribosomal protein L6, equivalent to O32998|MLCB2492_19 50S ribosomal protein L6 from Mycobacterium leprae (179 aa). Also highly similar to others e.g. P46786|RL6_STRCO|CAB82085.1|AL161803|SCD31.42 50S ribosomal protein L6 from Streptomyces coelicolor (179 aa), FASTA scores: opt: 872, E(): 0, (70.4% identity in 179 aa overlap); etc. Contains PS00525 Ribosomal protein L6 signature 1. Belongs to the L6P family of ribosomal proteins. P9WH81 No information pathways P66311 P9WH81 GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WH81 ML1844,ML1844c MMAR_1050 MSMEG_1470 Mb0740 NC_000962.3 Mycobrowser_v4 CDS 813940 814308 . + 0 Rv0720 rplR This is one of 3 proteins that mediate the attachment of the 5S RNA into the large ribosomal subunit. 50S ribosomal protein L18 RplR Rv0720, (MTCY210.39), len: 122 aa. rplR, 50S ribosomal protein L18, equivalent to O32999|MLCB2492_20|RL18_MYCLE 50S ribosomal protein L18 from Mycobacterium leprae (122 aa). Also highly similar to others e.g. CAB82086.1|AL161803 50S ribosomal protein L18 from Streptomyces coelicolor (127 aa); P33102|RL18_MICLU 50s ribosomal protein L18 from Micrococcus luteus (119 aa), FASTA scores: opt: 447, E(): 8.7e-24, (60.4% identity in 111 aa overlap); etc. Belongs to the L18P family of ribosomal proteins. P9WHD1 No information pathways P66076 P9WHD1 GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WHD1 ML1843,ML1843c MMAR_1051 MSMEG_1471 Mb0741 NC_000962.3 Mycobrowser_v4 CDS 2250996 2251883 . - 0 Rv2005c Rv2005c Unknown Universal stress protein family protein Rv2005c, (MTCY39.12), len: 295 aa. Universal stress protein family protein. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WLN1 No virulence, detoxification, adaptation P64921 P9WLN1 GO:0006950 P9WLN1 ML1421,ML1421c MMAR_2993 MSMEG_3945 Mb2028c NC_000962.3 Mycobrowser_v4 CDS 815190 815630 . + 0 Rv0723 rplO This protein binds directly to 23S ribosomal RNA. 50S ribosomal protein L15 RplO Rv0723, (MTCY210.42), len: 146 aa. rplO, 50S ribosomal protein L15, equivalent to MLCB2492_23|O33002 50S ribosomal protein L15 from Mycobacterium leprae (146 aa). Also highly similar to others e.g. P46787|RL15_STRCO|SCD31.46 50S ribosomal protein L15 from Streptomyces coelicolor (151 aa); P19946|RL15_BACSU 50s ribosomal protein L15 from Bacillus subtilis (146 aa), FASTA scores: opt: 419, E(): 6.5e-20, (51.0% identity in 145 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00475 Ribosomal protein L15 signature. Belongs to the L15P family of ribosomal proteins. P9WHD7 No information pathways P95071 P9WHD7 GO:0019843,GO:0003735,GO:0006412,GO:0015934 P9WHD7 ML1840,ML1840c MMAR_1054 MSMEG_1474 Mb0744 NC_000962.3 Mycobrowser_v4 CDS 814993 815190 . + 0 Rv0722 rpmD Involved ion translation mechanisms. 50S ribosomal protein L30 RpmD Rv0722, (MTCY210.41), len: 65 aa. rpmD, 50S ribosomal protein L30, equivalent to O33001 ribosomal protein L30 from Mycobacterium leprae (71 aa). Also highly similar to others e.g. P46789|RL30_STRCO 50S ribosomal protein L30 from Streptomyces coelicolor (60 aa); P02430|RL30_ECOLI 50S ribosomal protein L30 from Escherichia coli (58 aa), FASTA scores: opt: 168, E(): 1.5e-13, (53.7% identity in 54 aa overlap); etc. Belongs to the L30P family of ribosomal proteins. P9WHA3 No information pathways P66181 P9WHA3 GO:0003735,GO:0006412,GO:0015934 P9WHA3 ML1841,ML1841c MMAR_1053 MSMEG_1473 Mb0743 NC_000962.3 Mycobrowser_v4 CDS 830062 830610 . + 0 Rv0738 Rv0738 Function unknown Conserved protein Rv0738, (MTV041.12), len: 182 aa. Conserved protein, showing weak similarity with hypothetical proteins from Mycobacterium tuberculosis: Rv1727|MTCY04C12.12 (189 aa); MTY13D12_7|Z80343 hypothetical protein from Mycobacterium tuberculosis (194 aa), FASTA scores: opt: 172, E(): 0.0004, (24.2% identity in 178 aa overlap); and C-terminus of Rv0576. P9WKS3 No conserved hypotheticals P9WKS3 P9WKS3 MMAR_1076 Mb0759 NC_000962.3 Mycobrowser_v4 CDS 817539 818444 . - 0 Rv0725c Rv0725c Function unknown Conserved hypothetical protein Rv0725c, (MTCY210.44c), len: 301 aa. Conserved hypothetical protein, similar to hypothetical proteins from Mycobacterium tuberculosis e.g. Rv0726c, Rv0731c, Rv3399, etc, e.g. Y893_MYCTU|Q10552|Rv0893C hypothetical 36.1 kDa protein cy31.21c (325 aa), FASTA scores: opt: 600, E(): 3.9e-32, (43.8% identity in 219 aa overlap). P95073 No conserved hypotheticals P95073 P95073 GO:0008168 P95073 MMAR_1057 MSMEG_1479,MSMEG_1481 Mb0746c NC_000962.3 Mycobrowser_v4 CDS 818537 819640 . - 0 Rv0726c Rv0726c Possible methyltransferase Possible S-adenosylmethionine-dependent methyltransferase Rv0726c, (MTCY210.45c), len: 367 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), highly similar to other proteins from Mycobacterium tuberculosis e.g. Q10552|Y893_MYCTU|Rv0893c|MT0917|MTCY31.21c (325 aa), FASTA scores: opt: 646, E(): 0, (38.3% identity in 329 aa overlap); Rv0731c|MTV041.05c (318 aa), Rv3399, etc. Also similar to proteins from Mycobacterium leprae and other organisms e.g. T35930 hypothetical protein SC9B5.10 from Streptomyces coelicolor (303 aa). P9WFI7 No lipid metabolism P95074 P9WFI7 GO:0008168 2.1.1.- P9WFI7 MMAR_1059 Mb0747c NC_000962.3 Mycobrowser_v4 CDS 881459 882418 . + 0 Rv0787 Rv0787 Unknown Unknown protein Rv0787, (MTCY369.31), len: 319 aa. Unknown protein, equivalent to AAK45053.1 from Mycobacterium tuberculosis strain CDC1551 (242 aa) but longer 77 aa. P71840 No conserved hypotheticals P71840 ML2220,ML2220c MMAR_4900 MSMEG_6455 Mb0810 NC_000962.3 Mycobrowser_v4 CDS 819843 820499 . - 0 Rv0727c fucA Involved in fucose metabolism (at the third step) [catalytic activity: L-fuculose 1-phosphate = glycerone phosphate + (S)-lactaldehyde]. Possible L-fuculose phosphate aldolase FucA (L-fuculose-1-phosphate aldolase) Rv0727c, (MTV41.01c, MTCY210.46c), len: 218 aa. Possible fucA, L-fuculose-1-phosphate aldolase, similar to many e.g. NP_386339.1|NC_003047 putative L-fuculose phosphate aldolase protein from Sinorhizobium meliloti (222 aa); P11550|FUCA_ECOLI L-fuculose phosphate aldolase from Escherichia strain K12 (215 aa), FASTA scores: opt: 372, E(): 4.1e-19, (34.6% identity in 185 aa overlap); etc. Belongs to the aldolase class II family, ARAD/FUCA subfamily. Cofactor: binds one zinc ion per molecule. P95075 No intermediary metabolism and respiration P95075 P95075 GO:0046872,GO:0008738 4.1.2.17 P95075 ML1838 MMAR_1064 Mb0748c NC_000962.3 Mycobrowser_v4 CDS 820496 821476 . - 0 Rv0728c serA2 Involved at the first committed step in the 'phosphorylated' pathway of L-serine biosynthesis. Catalyzes the oxidation of D-3-phosphoglycerate to 3-phosphohydroxypyruvate [catalytic activity: 3-phosphoglycerate + NAD(+) = 3-phosphohydroxypyruvate + NADH]. Possible D-3-phosphoglycerate dehydrogenase SerA2 (phosphoglycerate dehydrogenase) (PGDH) Rv0728c, (MTV041.02c), len: 326 aa. Possible serA2, D-3-phosphoglycerate dehydrogenase, similar to others e.g. AF0278|AF027868_5|YoaD D-3-phosphoglycerate dehydrogenase from Bacillus subtilis (344 aa), FASTA scores: opt: 594, E(): 3.1e-31, (35.9% identity in 309 aa overlap); etc. Also similar to Rv2996c|MTV012.10|SERA1 D-3-phosphoglycerate dehydrogenase from Mycobacterium tuberculosis (528 aa). I6WZ71 No intermediary metabolism and respiration O86322 I6WZ71 GO:0055114,GO:0004617,GO:0051287 1.1.1.95 I6WZ71 ML1837 MMAR_1065 MSMEG_0604 Mb0749c NC_000962.3 Mycobrowser_v4 CDS 823683 824639 . - 0 Rv0731c Rv0731c Possible methyltransferase Possible S-adenosylmethionine-dependent methyltransferase Rv0731c, (MTV041.05c), len: 318 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), highly similar to other proteins from Mycobacterium tuberculosis e.g. Rv0726c|MTCY210.45c (367 aa), FASTA score: (60.9% identity in 317 aa overlap); Rv3399, Rv1729c, etc. P9WFI5 No lipid metabolism Q7D9D6 P9WFI5 GO:0008168 2.1.1.- P9WFI5 ML1834 MMAR_1069 Mb0752c NC_000962.3 Mycobrowser_v4 CDS 827543 828076 . + 0 Rv0735 sigL The sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released. Probable alternative RNA polymerase sigma factor SigL Rv0735, (MTV041.09), len: 177 aa. Probable sigL, alternative RNA polymerase sigma factor (rpoE) (see citations below), highly similar to many proteins of the extracytoplasmatic function (ECF) subfamily e.g. CAB72200.1|AL138851 putative RNA polymerase sigma factor from Streptomyces coelicolor (194 aa); Q06909|CARQ_MYXXA RNA polymerase sigma factor CARQ from Myxococcus xanthus (174 aa), FASTA scores: opt: 251, E(): 9.6e-11, (32.9% identity in 161 aa overlap); etc. Also similar to MTCI61_4, MTU87242_1, and MLU15180_30 from Mycobacterium tuberculosis. Contains PS01063 Sigma-70 factors ECF subfamily signature and probable helix-turn helix motif from aa 139-160 (Score 1134, +3.05 SD). Belongs to the sigma-70 factor family, ECF subfamily. P9WGH5 No information pathways 3HUG Q7D9D4 P9WGH5 GO:0016987,GO:0003700,GO:0006352,GO:0006355 P9WGH5 MMAR_1073 MSMEG_1486 Mb0756 NC_000962.3 Mycobrowser_v4 CDS 2314825 2315061 . - 0 Rv2058c rpmB2 Involved in ribosome activity 50S ribosomal protein L28 RpmB2 Rv2058c, (MTCY63A.02), len: 78 aa. rpmB2, 50S ribosomal protein L28, very similar to rL28 of M. tuberculosis. Also similar to rpmB (Rv0105c) of Mycobacterium tuberculosis. P9WHA9 No information pathways P66148 P9WHA9 GO:0003735,GO:0006412,GO:0005840 P9WHA9 MSMEG_6068 Mb2084c NC_000962.3 Mycobrowser_v4 CDS 826122 826667 . + 0 Rv0733 adk This small ubiquitous enzyme is essential in intracellular nucleotide metabolism, in addition it has been found to act as both a nucleoside mono- and DI-phosphate kinase suggesting it may have a role in RNA and DNA biosynthesis [catalytic activity: ATP + AMP = ADP + ADP]. Adenylate kinase Adk (ATP-AMP transphosphorylase) Rv0733, (MTV041.07), len: 181 aa. adk, adenylate kinase (ATP-AMP transphosphorylase), equivalent to Z98756|MLCB24 92_28 probable adenylate kinase from Mycobacterium leprae (181 aa), FASTA scores: opt: 978, E(): 0, (83.6% identity in 177 aa overlap); and AAF86323.1|AF271342 putative adenylate kinase from Mycobacterium marinum (124 aa) (N-terminus shorter). Also highly similar to others e.g. P43414|KAD_STRCO adenylate kinase from Streptomyces coelicolor (217 aa), FASTA score: (43.0% identity in 186 aa overlap); etc. Contains PS00113 Adenylate kinase signature. Belongs to the adenylate kinase family. P9WKF5 No intermediary metabolism and respiration 1P4S,2CDN P69440 P9WKF5 GO:0004017,GO:0005737,GO:0009165,GO:0005524 2.7.4.3 P9WKF5 ML1832,ML1832c MMAR_1071 MSMEG_1484 Mb0754 NC_000962.3 Mycobrowser_v4 CDS 826670 827470 . + 0 Rv0734 mapA Removes the amino-terminal methionine from nascent proteins [catalytic activity: L-methionylpeptide + H2O = L-methionine + peptide]. Methionine aminopeptidase MapA (map) (peptidase M) (MetAP) Rv0734, (MTV041.08), len: 266 aa. mapA, methionine aminopeptidase (map), equivalent to Z98756|MLCB2492_29 probable methionine aminopeptidase from Mycobacterium leprae (266 aa), FASTA scores: opt: 1717, E(): 0, (83.4% identity in 265 aa overlap). Also highly similar to many e.g. T35553 methionine aminopeptidase from Streptomyces coelicolor (278 aa); etc. Also similar to Rv2861c|MAPB probable methionine aminopeptidase from Mycobacterium tuberculosis (285 aa). Belongs to peptidase family M24A; also known as the map family 1. Cofactor: cobalt; binds 2 ions per subunit. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P9WK21 No intermediary metabolism and respiration Q7D9D5 P9WK21 GO:0009987,GO:0046872,GO:0008235,GO:0006508,GO:0004177 3.4.11.18 P9WK21 ML1831,ML1831c MMAR_1072 MSMEG_1485 Mb0755 NC_000962.3 Mycobrowser_v4 CDS 829207 829704 . + 0 Rv0737 Rv0737 Involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0737, (MTV041.11), len: 165 aa. Possible transcriptional regulator, similar to others e.g. BAB69161.1|AB070937 regulator protein from Streptomyces avermitilis (169 aa); NP_419731.1|NC_002696 transcriptional regulator MarR family from Caulobacter crescentus (148 aa) (homology only at C-terminus); etc. Also shows weak similarity to AB0014|AB001488_14 hypothetical protein from Bacillus subtilis (164 aa), FASTA scores: opt: 163, E(): 9.3e-05, (32.8% identity in 116 aa overlap), which is similar to slyY gene of S. typhimurium required for survival in macrophage. Contains possible helix-turn helix motif from aa 73-94 (Score 1138, +3.06 SD). I6Y8K3 No regulatory proteins Q7D9D2 I6Y8K3 GO:0006355,GO:0006350,GO:0003700,GO:0005622 I6Y8K3 MMAR_1075 MSMEG_1492 Mb0758 NC_000962.3 Mycobrowser_v4 CDS 830855 831661 . + 0 Rv0739 Rv0739 Function unknown Conserved hypothetical protein Rv0739, (MTV041.13), len: 268 aa. Conserved hypothetical protein, showing some similarity to Mycobacterium tuberculosis proteins Rv0026 (448 aa), FASTA score: (37.6% identity in 101 aa overlap)and Rv0025 (120 aa), FASTA score: (32.4% identity in 142 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WKS1 No conserved hypotheticals O53802 P9WKS1 P9WKS1 Mb0760 NC_000962.3 Mycobrowser_v4 CDS 1595979 1596884 . + 0 Rv1421 Rv1421 Function unknown Conserved protein Rv1421, (MTCY21B4.39), len: 301 aa. Conserved protein, similar to many hypothetical proteins e.g. YHBJ_ECOLI|P33995 hypothetical 32.5 kd protein from Escherichia coli (284 aa), FASTA scores: opt: 648, E(): 6.3e-36, (38.7% identity in 282aa overlap). P9WFQ3 No conserved hypotheticals P67106 P9WFQ3 GO:0005525,GO:0005524 P9WFQ3 ML0563 MMAR_2228 MSMEG_3079 Mb1456 NC_000962.3 Mycobrowser_v4 CDS 2291269 2291925 . + 0 Rv2046 lppI Unknown Probable lipoprotein LppI Rv2046, (MTV018.33), len: 218 aa. Probable lppI, lipoprotein contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O53489 No cell wall and cell processes O53489 ML1435 MMAR_3021 MSMEG_3851 Mb2072 NC_000962.3 Mycobrowser_v4 CDS 2546102 2546431 . + 0 Rv2273 Rv2273 Unknown Probable conserved transmembrane protein Rv2273, (MTCY339.37c), len: 109 aa. Probable conserved transmembrane protein, similar to Rv2272 (MTCY339.38c), (35.0% identity in 100 aa overlap). P9WLF3 No cell wall and cell processes P64971 P9WLF3 GO:0005886,GO:0016021 ML1790 MMAR_3369 MSMEG_1230 Mb2296 NC_000962.3 Mycobrowser_v4 CDS 832534 832848 . + 0 Rv0741 Rv0741 Required for the transposition of the insertion element IS1557. Probable transposase (fragment) Rv0741, (MTV041.15), len: 104 aa. Probable truncated transposase for IS1557, showing similarity to transposases and is elements e.g. U63997|EFU63997_1 insertion sequence from Enterococcus faecium (424 aa), FASTA score: (31.0% identity in 87 aa overlap). Very high similarity with the C-terminal part of Z73419|MTCY373_3 2 IS1557 from Mycobacterium tuberculosis (444 aa), FASTA score: (86.5% identity in 104 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6X9N4 No insertion seqs and phages I6X9N4 Mb0762 NC_000962.3 Mycobrowser_v4 CDS 832981 833508 . + 0 Rv0742 PE_PGRS8 Function unknown PE-PGRS family protein PE_PGRS8 Rv0742, (MTV041.16), len: 175 aa. PE_PGRS8, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), similar to many Mycobacterium tuberculosis PGRS-type proteins e.g. Z78020|MTCY1A11_25 (498 aa), FASTA scores: opt: 766, E(): 6.1e-25, (73.6% identity in 178 aa overlap). Similarity suggests ORF starts with ATA start codon. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6Y8K5 No PE/PPE Q79FW9 I6Y8K5 I6Y8K5 Mb0763 NC_000962.3 Mycobrowser_v4 CDS 833886 834443 . - 0 Rv0743c Rv0743c Unknown Hypothetical protein Rv0743c, (MTV041.17c), len: 185 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6WZ83 No conserved hypotheticals I6WZ83 I6WZ83 Mb0764c NC_000962.3 Mycobrowser_v4 CDS 835154 835681 . + 0 Rv0745 Rv0745 Function unknown Conserved hypothetical protein Rv0745, (MTV041.19), len: 175 aa. Conserved hypothetical protein; shows high similarity to a 50 aa region of Rv3649|Z95436|MTY15C10_3 conserved hypothetical protein, similar to ATP-dependent helicases, from Mycobacterium tuberculosis (771 aa), FASTA scores: opt: 225, E(): 7e-06, (70.0% identity in 50 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6X9N8 No conserved hypotheticals I6X9N8 Mb0766 NC_000962.3 Mycobrowser_v4 CDS 835701 838052 . + 0 Rv0746 PE_PGRS9 Function unknown PE-PGRS family protein PE_PGRS9 Rv0746, (MTV041.20), len: 783 aa. PE_PGRS9, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to part of MTCY28.25c|Rv1759c|Z95890 antigen wag22 from M. tuberculosis (914 aa), FASTA scores: opt: 2429, E(): 0, (56.9% identity in 873 aa overlap). Also similar to other PE-PGRS family proteins e.g. AL0212|MTV008_46 FASTA score: (48.8% identity in 887 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q79FW8 No PE/PPE Q79FW8 Q79FW8 Q79FW8 MMAR_1195,MMAR_1391,MMAR_1850,MMAR_1851,MMAR_2695,MMAR_2948,MMAR_3326,MMAR_4398,MMAR_5207 Mb0767 NC_000962.3 Mycobrowser_v4 CDS 1419014 1419748 . - 0 Rv1270c lprA Unknown Possible lipoprotein LprA Rv1270c, (MTCY50.12), len: 244 aa. Possible lprA, lipoprotein. Similar to O32852|AJ000500 lipoprotein from Mycobacterium bovis (236 aa), fasta scores: E(): 5.2e-23, (35.1% identity in 245 aa overlap). Similar to M. tuberculosis lipoproteins: Rv1368, Rv1411c, Rv2945c. Contains probable N-terminal signal sequence. P9WK55 No cell wall and cell processes Q11049 P9WK55 GO:0005886 P9WK55 MMAR_4152 Mb1301c NC_000962.3 Mycobrowser_v4 CDS 2314354 2314659 . - 0 Rv2056c rpsN2 Involved in translation 30S ribosomal protein S14 RpsN2 Rv2056c, (MTCY63A.04), len: 101 aa. rpsN2, 30S ribosomal protein S14, similar to many. Also similar to rpsN|Rv0717|MTCY210.36 from Mycobacterium tuberculosis, (50.0% identity in 62 aa overlap). P9WH59 No information pathways P66405 P9WH59 GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WH59 MMAR_0290 MSMEG_6066 Mb2082c NC_000962.3 Mycobrowser_v4 CDS 840947 841204 . + 0 Rv0748 vapB31 Unknown Possible antitoxin VapB31 Rv0748, (MTV041.22), len: 85 aa. Possible vapB31, antitoxin, part of toxin-antitoxin (TA) operon with Rv0749, see Arcus et al. 2005. Also similar to others in Mycobacterium tuberculosis proteins e.g. Rv2871 (75 aa); Rv1241, Rv2132, Rv3321c, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53811 No virulence, detoxification, adaptation O53811 O53811 GO:0006355,GO:0003677 O53811 Mb0769 NC_000962.3 Mycobrowser_v4 CDS 841228 841656 . + 0 Rv0749 vapC31 Unknown Possible toxin VapC31. Contains PIN domain. Rv0749, (MTV041.23), len: 142 aa. Possible vapC31, toxin, part of toxin-antitoxin (TA) operon with Rv0748, contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. Rv0277c, Rv2530c, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WF75 No virulence, detoxification, adaptation O53812 P9WF75 P9WF75 Mb0770 NC_000962.3 Mycobrowser_v4 CDS 841737 841874 . - 0 Rv0749A Rv0749A Unknown Hypothetical protein (fragment) Rv0749A, len: 45 aa. Conserved hypothetical protein (probably gene fragment), similar to part (aa 250-292) of Rv2807|Z81331_12 from Mycobacterium tuberculosis (384 aa), FASTA scores: opt: 238, E(): 1.9e-13, (79.07% identity in 43 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6X9Q1 No conserved hypotheticals I6X9Q1 Mb0771c NC_000962.3 Mycobrowser_v4 CDS 843242 844414 . - 0 Rv0752c fadE9 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE9 Rv0752c, (MTV041.26c), len: 390 aa. Probable fadE9, acyl-CoA dehydrogenase, highly similar to many e.g. NP_437985.1|NC_003078 putative acyl-CoA dehydrogenase protein from Sinorhizobium meliloti (380 aa); Z99123|BSUB0020_14 from Bacillus subtilis (379 aa), FASTA scores: opt: 853, E(): 0, (39.8% identity in 384 aa overlap); etc. Contains PS00072 Acyl-CoA dehydrogenases signature 1, and PS00073 Acyl-Co Adehydrogenases signature 2. Belongs to the acyl-CoA dehydrogenases family. I6Y4R2 No lipid metabolism O53815 I6Y4R2 GO:0003995,GO:0055114,GO:0050660 1.3.99.- I6Y4R2 MMAR_1078 MSMEG_1497 Mb0774c NC_000962.3 Mycobrowser_v4 CDS 863256 864143 . + 0 Rv0770 Rv0770 Function unknown; 3-hydroxyisobutyrate dehydrogenase family protein probably involved in cellular metabolism. Probable dehydrogenase/reductase Rv0770, (MTCY369.15), len: 295 aa. Probable dehydrogenase/reductase, 3-hydroxyisobutyrate dehydrogenase family, possibly 3-hydroxyisobutyrate dehydrogenase or 2-hydroxy-3-oxopropionate reductase, similar to others e.g. P23523|GARR_ECOLI 2-hydroxy-3-oxopropionate reductase (tartronate semialdehyde reductase) (TSAR) from Escherichia coli strain K12 (294 aa), FASTA scores: opt: 469, E(): 6.7e-22, (34.4% identity in 282 aa overlap); P28811|MMSB_PSEAE 3-hydroxyisobutyrate dehydrogenase (hibadh) from Pseudomonas aeruginosa (298 aa), FASTA scores: opt: 439, E(): 4.3e-20, (34.9% identity in 269 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. Rv1122 and Rv1844c. Seems to belong to the 3-hydroxyisobutyrate dehydrogenase family. P9WNY3 No intermediary metabolism and respiration P71825 P9WNY3 GO:0050662,GO:0055114,GO:0006098,GO:0004616,GO:0006573,GO:0008442 1.1.-.- P9WNY3 MMAR_4926 MSMEG_5857 Mb0793 NC_000962.3 Mycobrowser_v4 CDS 2258854 2259285 . - 0 Rv2011c Rv2011c Unknown Conserved hypothetical protein, probable transcription repressor. Rv2011c, (MTCY39.06), len: 143 aa. Conserved hypothetical protein, probable transcription repressor. Contains IPR011991 Winged helix-turn-helix transcription repressor DNA-binding domain. P9WLM5 No regulatory proteins P9WLM5 P9WLM5 Mb2034c NC_000962.3 Mycobrowser_v4 CDS 846159 847913 . + 0 Rv0754 PE_PGRS11 Function unknown PE-PGRS family protein PE_PGRS11 Rv0754, (MTV041.28), len: 584 aa. PE_PGRS11, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), similar to others e.g. AL0212|MTV008_46 from Mycobacterium tuberculosis (1660 aa), FASTA score: (48.7% identity in 345 aa overlap); Z80225|MTCY441_4 from Mycobacterium tuberculosis (778 aa), FASTA score: (41.6% identity in 442 aa overlap); etc. Q79FW5 No PE/PPE Q79FW5 Q79FW5 Q79FW5 ML2241,ML2241c MMAR_4953 Mb0776 NC_000962.3 Mycobrowser_v4 CDS 848103 850040 . - 0 Rv0755c PPE12 Function unknown PPE family protein PPE12 Rv0755c, (MTV041.29), len: 645 aa. PPE12, Member of the Mycobacterium tuberculosis PPE family, highly similar to others e.g. Z82098|MTCY3C7_23 from Mycobacterium tuberculosis (582 aa), FASTA scores: (56.1% identity in 636 aa overlap); Z92774|MTCY6G11_5 from Mycobacterium tuberculosis (552 aa), FASTA scores: (55.8% identity in 590 aa overlap); etc. Predicted to be an outer membrane protein (See Song et al., 2008). P9WI37 No PE/PPE Q79FW4 P9WI37 P9WI37 Mb0777c NC_000962.3 Mycobrowser_v4 CDS 850342 850527 . - 0 Rv0755A Rv0755A Could be required for the transposition of an insertion element. Putative transposase (fragment) Rv0755A, len: 61 aa. Putative transposase (possibly gene fragment), similar to C-terminal part of Q9EZM2|ISMav2|AF286339_1 putative transposase from Mycobacterium paratuberculosis (395 aa), FASTA scores: opt: 284, E(): 5e-13, (83.02% identity in 53 aa overlap); and to SCJ11.25c|Q9RI80 possible noncomposite transposon transposase from Streptomyces coelicolor (283 aa). Q79FW3 No insertion seqs and phages Q79FW3 Q79FW3 GO:0004803,GO:0006313,GO:0003677 Mb0778c NC_000962.3 Mycobrowser_v4 CDS 868984 869763 . - 0 Rv0776c Rv0776c Function unknown Conserved hypothetical protein Rv0776c, (MTCY369.21a), len: 259 aa. Conserved hypothetical protein, similar (except first 50 aa) to P72737|D90900_57 hypothetical protein from Synechocystis sp. strain PCC 6803 (261 aa), FASTA scores: opt: 337, E(): 1.7e-15, (30.5% identity in 266 aa overlap). I6Y4S9 No conserved hypotheticals P71831 I6Y4S9 ML2231 MMAR_4917 Mb0799c NC_000962.3 Mycobrowser_v4 CDS 878638 879324 . + 0 Rv0784 Rv0784 Function unknown Conserved hypothetical protein Rv0784, (MTC369.28), len: 228 aa. Conserved hypothetical protein, with some similarity to MLCB5_20|O05752 hypothetical protein from Mycobacterium leprae (193 aa), FASTA scores: opt: 141, E(): 0.0022, (36.0% identity in 114 aa overlap). Also similar to N-terminus of NP_253002.1|NC_002516 conserved hypothetical protein from Pseudomonas aeruginosa (253 aa). P71837 No conserved hypotheticals P71837 P71837 ML2223,ML2223c MMAR_4904 MSMEG_5836 Mb0806 NC_000962.3 Mycobrowser_v4 CDS 853825 854157 . - 0 Rv0759c Rv0759c Function unknown Conserved hypothetical protein Rv0759c, (MTCY369.04c), len: 110 aa. Conserved hypothetical protein, highly similar (but shorter 45 aa in N-terminus) to P49774|YHIT_MYCLE|ML2237|MLCB5.04c|U296A hypothetical hit-like protein from Mycobacterium leprae (155 aa), FASTA scores: opt: 766, E(): 0, (78.7% identity in 150 aa overlap). Also highly similar (but N-terminus always shorter) to hit-like proteins and protein kinase inhibitors e.g. AAF72728.1|AF265258_1|AF265258 hit-like protein from Rhodococcus sp. (141 aa); NP_212513.1|NC_001318 protein kinase C1 inhibitor (pkcI) from Borrelia burgdorferi (149 aa) ; P94252|YHIT_BORBU|BB0379 hypothetical hit-like protein from Borrelia burgdorferi (139 aa); NP_110768.1|NC_002689 hit (histidine triad) family protein from Thermoplasma volcanium (158 aa); P16436|IPK1_BOVIN protein kinase C inhibitor 1 (pkci-1) from Bos taurus (Bovine) (125 aa), FASTA scores: opt: 195, E(): 5.2e-08, (33.3% identity in 111 aa overlap); etc. Also shows similarity with Rv2613c|MTCY01A10.20A conserved hypothetical protein from Mycobacterium tuberculosis (195 aa) and Rv1262c|MTCY50.20 hypothetical hit-like protein (144 aa). P9WML3 No conserved hypotheticals P0A5B5 P9WML3 GO:0003824 P9WML3 ML2237 MMAR_4940 MSMEG_5871 Mb0782c NC_000962.3 Mycobrowser_v4 CDS 854267 854686 . - 0 Rv0760c Rv0760c Function unknown Conserved protein Rv0760c, (MTCY369.05), len: 139 aa. Conserved protein, similar to N-terminal part of Rv2042c conserved hypothetical protein from Mycobacterium tuberculosis (265 aa), FASTA scores: opt: 150, E(): 4.1e-05, (28.7% identity in 136 aa overlap). Belongs to the NTF2-like (nuclear transport factor 2) protein superfamiily. I6WZD7 No conserved hypotheticals 2A15,2Z76,2Z77,2Z7A P71817 I6WZD7 GO:0016853,GO:0006810,GO:0005622 I6WZD7 MMAR_4937 MSMEG_5867 Mb0783c NC_000962.3 Mycobrowser_v4 CDS 855925 856470 . - 0 Rv0762c Rv0762c Function unknown Conserved hypothetical protein Rv0762c, (MTCY369.07c), len: 181 aa. Conserved hypothetical protein, showing weak similarity to D90907_77|P73575 hypothetical 31.3KD protein from Synechocystis sp, FASTA scores: E(): 0.0012, (30.4% identity in 92 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). I6XW93 No conserved hypotheticals I6XW93 I6XW93 MMAR_4934 MSMEG_5865 Mb0785c NC_000962.3 Mycobrowser_v4 CDS 867440 868351 . - 0 Rv0774c Rv0774c Unknown; could have possibly a lipolytic activity. Probable conserved exported protein Rv0774c, (MTCY369.19c), len: 303 aa. Possible conserved exported protein with hydrophobic region near N-terminus, highly similar, except in N-terminus, to Rv0519c|Z97831|MTY20G10.09c|O33364 hypothetical protein from Mycobacterium tuberculosis (300 aa), FASTA scores: opt: 1092, E(): 0, (57.9% identity in 299 aa overlap). Contains PS00061 Short-chain alcohol dehydrogenase family signature, and PS00120 Lipases, serine active site. So could be a lipase. Start changed since first submission (-9 aa). Predicted to be an outer membrane protein (See Song et al., 2008). I6Y8R4 No cell wall and cell processes P71829 I6Y8R4 I6Y8R4 ML2233 MMAR_4919 MSMEG_5851 Mb0797c NC_000962.3 Mycobrowser_v4 CDS 1243010 1243504 . - 0 Rv1120c Rv1120c Function unknown Conserved hypothetical protein Rv1120c, (MTCY22G8.09c), len: 164 aa. Conserved hypothetical protein, some similarity at C-terminus to Mycobacterium tuberculosis hypothetical proteins e.g. Rv1890c|MTCY180.28 (462 aa), FASTA scores: opt: 187, E(): 2.2e-05, (36.6% identity in 93 aa overlap) and Rv2488c|YZ19_MYCTU|Q10551 (285 aa), FASTA scores: opt: 156, E(): 0.00074, (32.7% identity in 107 aa overlap). O06572 No conserved hypotheticals O06572 O06572 GO:0016849,GO:0009190 O06572 Mb1151c NC_000962.3 Mycobrowser_v4 CDS 856473 856679 . - 0 Rv0763c Rv0763c Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Probably involved in electron transport for cytochrome P-450 system. Possible ferredoxin Rv0763c, (MTCY369.08c), len: 68 aa. Possible ferredoxin, similar to others and related proteins e.g. P18324|FER1_STRGO|SUAB ferredoxin 1 (fd-1) from Streptomyces griseolus (68 aa); AAK31349.1|AF350429_2|AF350429 putative ferredoxin from Nocardioides sp (63 aa); AAK16536.1|AF331043_16|AF331043 phthalate dioxygenase ferredoxin subunit from Arthrobacter keyseri (64 aa); etc. Probably involved in electron transport for cytochrome P-450 system e.g. downstream ORF Rv0764c|MTCY369.09c probable cytochrome P450 51 from Mycobacterium tuberculosis (451 aa), FASTA scores: opt: 137, E(): 0.00013, (36.4% identity in 66 aa overlap). Also similar to putative ferredoxins Rv3503c and Rv1786 from Mycobacterium tuberculosis. Could belong to the bacterial type ferredoxin family. P71820 No intermediary metabolism and respiration P71820 P71820 P71820 MSMEG_5864 Mb0786c NC_000962.3 Mycobrowser_v4 CDS 858037 858864 . - 0 Rv0765c Rv0765c Function unknown; probably involved in cellular metabolism, possibly electron transfer. Probable oxidoreductase Rv0765c, (MTCY369.10c), len: 275 aa. Probable oxidoreductase, similar others e.g. P39071|DHBA_BACSU 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase from Bacillus subtilis (261 aa), FASTA scores: opt: 385, E(): 1.8e-17, (30.6% identity in 252 aa overlap); AAF81239.1|AF263012 putative beta-ketoacyl reductase from Streptomyces griseus (274 aa); NP_436514.1|NC_003037 putative oxidoreductase from Sinorhizobium meliloti (240 aa); etc. Also similar to several other oxidoreductases from Mycobacterium tuberculosis e.g. Rv1544|MTCY48.21, FASTA score: (32.6% identity in 267 aa overlap); etc. Contains PS00061 Short-chain alcohol dehydrogenase family signature. I6WZD9 No intermediary metabolism and respiration P71821 I6WZD9 GO:0055114,GO:0016491 1.-.-.- I6WZD9 MMAR_4931 MSMEG_5862 Mb0788c NC_000962.3 Mycobrowser_v4 CDS 858864 860072 . - 0 Rv0766c cyp123 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Probable cytochrome P450 123 Cyp123 Rv0766c, (MT0790, MTCY369.11c), len: 402 aa. Probable cyp123, cytochrome P-450, similar to others e.g. P33271|CPXK_SACER cytochrome P-450 107B1 from Saccharopolyspora erythraea (405 aa), FASTA scores: opt: 770, E(): 0, (36.9% identity in 406 aa overlap); T36526 probable cytochrome P450 hydroxylase from Streptomyces coelicolor (411 aa); P27632|CPXM_BACSU cytochrome P450 109 from Bacillus subtilis (405 aa); etc. Also similar to several other cytochromes P-450 from Mycobacterium tuberculosis e.g. Rv1256c|MTCY50.26 (405 aa), FASTA score: (35.2% identity in 389 aa overlap); etc. Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. Belongs to the cytochrome P450 family. P9WPP5 No intermediary metabolism and respiration P63707 P9WPP5 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPP5 MMAR_4930 MSMEG_5861 Mb0789c NC_000962.3 Mycobrowser_v4 CDS 860069 860710 . - 0 Rv0767c Rv0767c Function unknown Conserved hypothetical protein Rv0767c, (MTCY369.12c), len: 213 aa. Conserved hypothetical protein, showing weak similarity with AL133220|SCC75A_26 hypothetical protein from Streptomyces coelicolor (215 aa), FASTA scores: opt: 152, E(): 0.0048, (28.4% identity in 204 aa overlap). P9WMD7 No conserved hypotheticals P67432 P9WMD7 GO:0006350,GO:0003700,GO:0006355 P9WMD7 MMAR_4929 MSMEG_5860 Mb0790c NC_000962.3 Mycobrowser_v4 CDS 864140 864574 . + 0 Rv0771 Rv0771 Involved in aromatic hydrocarbons catabolism. Thought to be involved in the catabolism of protocatechuate to succinate-and acetyl-CoA in the beta-ketoadipate pathway (at the third step) [catalytic activity: 2-carboxy-5-oxo-2,5-dihydrofuran-2-acetate = 5-oxo-4,5-dihydrofuran-2-acetate + CO(2)]. Possible 4-carboxymuconolactone decarboxylase (CMD) Rv0771, (MTCY369.16), len: 144 aa. Possible 4-carboxymuconolactone decarboxylase, showing similarity with other carboxymuconolactone decarboxylases e.g. AAD39557.1|AF031417 PcaC-like protein from Pseudomonas putida (130 aa); P20370|DC4C_ACICA 4-carboxymuconolactone decarboxylase (CMD) from Acinetobacter sp. ADP1 (134 aa), FASTA scores: opt: 174, E(): 0.00075, (31.4% identity in 121 aa overlap); C-terminus of NP_421214.1|NC_002696 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase from Caulobacter crescentus (393 aa); C-terminus of T47115 probable 4-carboxymuconolactone decarboxylase / 3-oxoadipate enol-lactone hydrolase from Streptomyces sp (373 aa); NP_407104.1|NC_003143 putative gamma carboxymuconolactone decarboxylase from Yersinia pestis (131 aa); etc. I6Y4S7 No intermediary metabolism and respiration P71826 I6Y4S7 GO:0047575 4.1.1.44 I6Y4S7 MMAR_4925 MSMEG_5856 Mb0794 NC_000962.3 Mycobrowser_v4 CDS 865851 867389 . - 0 Rv0773c ggtA Besides the cephalosporin acylase I activity which converts GL-7ACA into 7-ACA; this enzyme displays some gamma glutamyltranspeptidase activity: GGT plays a key role in the gamma-glutamyl cycle, a pathway for the synthesis and degradation of glutathione. [catalytic activity 1: 7-beta-(4-carboxybutanamido)-cephalosporanic acid + H2O = 7-aminocephalosporanic acid + glutaric acid] [catalytic activity 2: (5-L-glutamyl)-peptide + an amino acid = peptide + 5-L-glutamyl-amino acid]. Probable bifunctional acylase GgtA: cephalosporin acylase (GL-7ACA acylase) + gamma-glutamyltranspeptidase (GGT) Rv0773c, (MTCY369.18), len: 512 aa. Probable ggtA, bifunctional acylase including cephalosporin acylase, and gamma-glutamyl transpeptidase; highly similar to others e.g. NP_295247.1|NC_001263 cephalosporin acylase from Deinococcus radiodurans (535 aa); NP_248854.1|NC_002516 probable gamma-glutamyltranspeptidase from Pseudomonas aeruginosa (538 aa); P15557|PAC1_PSES3 acylase ACY 1 [includes: cephalosporin acylase (GL-7ACA acylase); gamma-glutamyltranspeptidase (GGT)] from Pseudomonas sp. strain SE83 (558 aa), FASTA scores: opt: 784, E(): 0, (34.2% identity in 526 aa overlap); NP_391491.1|NC_000964|Z93767|BSZ93767_6|O0521 protein similar to gamma-glutamyltransferase from Bacillus subtilis (525 aa), FASTA scores: opt: 1169, E(): 0, (40.1% identity in 516 aa overlap); etc. Also similar to Rv2394|ggtB from Mycobacterium tuberculosis. Member of GL-7ACA acylases and to GGT group. I6X9S5 No intermediary metabolism and respiration P71828 I6X9S5 GO:0003840,GO:0016787,GO:0008415 3.5.1.-,2.3.2.2 I6X9S5 ML2234 MMAR_4920 MSMEG_1463 Mb0796c NC_000962.3 Mycobrowser_v4 CDS 868407 869030 . + 0 Rv0775 Rv0775 Function unknown Conserved hypothetical protein Rv0775, (MTCY369.20), len: 207 aa. Conserved hypothetical protein, showing some similarity to other proteins e.g. ECAE000186_11|MG1655 hypothetical protein from Escherichia coli strain K-12 (178 aa), FASTA scores: E(): 6.4e-05, (27.2% identity in 147 aa overlap); P41037|BIH_ECOLI hypothetical transcriptional regulator from Escherichia coli (103 aa), FASTA scores: opt: 138, E(): 0.003, (30.9% identity in 97 aa overlap); etc. P71830 No conserved hypotheticals P71830 P71830 GO:0006350,GO:0003700,GO:0006355 P71830 ML2232,ML2232c MMAR_4918 MSMEG_5850 Mb0798 NC_000962.3 Mycobrowser_v4 CDS 2330214 2330963 . - 0 Rv2073c Rv2073c Unknown Probable shortchain dehydrogenase Rv2073c, (MTCY49.12c), len: 249 aa. Probable oxidoreductase, belonging to shortchain dehydrogenase reductase (SDR) family, similar to many. P9WGR3 No intermediary metabolism and respiration Q10681 P9WGR3 GO:0055114,GO:0016491 1.-.-.- P9WGR3 MMAR_3055 MSMEG_3879 NC_000962.3 Mycobrowser_v4 CDS 871431 872675 . + 0 Rv0778 cyp126 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Possible cytochrome P450 126 Cyp126 Rv0778, (MT0802, MTCY369.22), len: 414 aa. Possible cyp126, cytochrome P-450, similar to other cytochromes and related proteins e.g. AAG29781.1|AF235050_4|AF235050 cytochrome P-450 from Streptomyces rishiriensis (407 aa); Q59723|PSECYTOCHR_1 cytochrome p-450 linalool 8-monooxygenase (lin C) from Pseudomonas incognita (406 aa), FASTA scores: opt: 769, E(): 0, (37.0% identity in 411 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. Rv0766c, Rv2266, Rv3545c, etc. Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. P9WPN9 No intermediary metabolism and respiration P63711 P9WPN9 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPN9 ML2229,ML2229c MMAR_4915 MSMEG_5846 Mb0801 NC_000962.3 Mycobrowser_v4 CDS 872672 873292 . - 0 Rv0779c Rv0779c Unknown Possible conserved transmembrane protein Rv0779c, (MTCY369.23c), len: 206 aa. Possible conserved transmembrane protein, equivalent to Z95151|MLCB5_14 O05747 conserved hypothetical protein from Mycobacterium leprae (206 aa), FASTA scores: opt: 902, E(): 0, (67.2% identity in 204 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P71833 No cell wall and cell processes P71833 GO:0016021 ML2228 MSMEG_5842 Mb0802c NC_000962.3 Mycobrowser_v4 CDS 889072 889398 . + 0 Rv0795 Rv0795 Required for the transposition of the insertion element IS6110. Putative transposase for insertion sequence element IS6110 (fragment) Rv0795, (MTV042.05), len: 108 aa. Putative transposase for IS6110 (fragment), identical to Q50686 insertion element IS6110 (108 aa), FASTA score: (100.0 % identity in 108 aa overlap). The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv0795 and Rv0796, the sequence UUUUAAAG (directly upstream of Rv0796) maybe responsible for such a frameshifting event (see McAdam et al., 1990). P9WKH5 No insertion seqs and phages P0C5G9 P9WKH5 GO:0004803,GO:0006313,GO:0003677 P9WKH5 NC_000962.3 Mycobrowser_v4 CDS 910972 911739 . + 0 Rv0818 Rv0818 Involved in transcriptional mechanism. Transcriptional regulatory protein Rv0818, (MTV043.10), len: 255 aa. Probable transcriptional regulatory protein, highly similar to Q05943|GLNR_STRCO|L03213|STMGLNR_1|SCD84.26c transcriptional regulatory protein from Streptomyces coelicolor (267 aa), FASTA scores: opt: 945, E(): 0, (61.5 identity in 239 aa overlap); and similar to others from other organisms. Also similar to Rv2884|MTCY274.15|Z74024 from Mycobacterium tuberculosis (252 aa), FASTA scores: opt: 662, E(): 0, (47.8% identity in 226 aa overlap). O53830 No regulatory proteins O53830 O53830 GO:0005622,GO:0006355,GO:0000156,GO:0000160,GO:0003677 O53830 ML2194,ML2194c MMAR_4865 MSMEG_5784 Mb0841 NC_000962.3 Mycobrowser_v4 CDS 1029513 1030577 . - 0 Rv0923c Rv0923c Function unknown Conserved hypothetical protein Rv0923c, (MTCY21C12.17c), len: 354 aa. Conserved hypothetical protein, showing similarity with C-terminal part of AF034138|AF034138_7|yjoB hypothetical protein from Bacillus subtilis (200 aa), FASTA scores: opt: 193, E(): 4.2e-05, (32.3% identity in 167 aa overlap). I6XWK6 No conserved hypotheticals O05915 I6XWK6 I6XWK6 MMAR_4586 MSMEG_5591 Mb0947c NC_000962.3 Mycobrowser_v4 CDS 874732 876390 . + 0 Rv0782 ptrBb Cleaves peptide bonds on the C-terminal side of lysyl and argininyl residues [catalytic activity: hydrolysis of ARG-|-XAA and LYS-|-XAA bonds in oligopeptides, even when P1' residue is proline]. Probable protease II PtrBb [second part] (oligopeptidase B) Rv0782, (MTCY369.26), len: 552 aa. Probable ptrBb, second part of protease II, equivalent to C-terminus of NP_302455.1|NC_002677 protease II from Mycobacterium leprae (724 aa). Also highly similar to N-termini of many proteases II e.g. P24555|PTRB_ECOLI|TLP|B1845 protease II from Escherichia coli strains K12 and HB101 (707 aa), FASTA scores: opt: 1251, E(): 0, (42.7% identity in 489 aa overlap); etc. ORFs Rv0782 and Rv0781 appear to be a frameshifted homologues of protease II, but we can find no error in the cosmid sequence to account for this. Belongs to peptidase family S9A; also known as the prolyl oligopeptidase family. Note that previously known as ptrBa. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P71834 No intermediary metabolism and respiration P71834 P71834 GO:0004252,GO:0006508 3.4.21.83 P71834 ML2226,ML2226c MMAR_4908 MSMEG_5839 Mb0804 NC_000962.3 Mycobrowser_v4 CDS 882760 883434 . + 0 Rv0788 purQ Involved in de novo purine biosynthesis (at the fourth step) [catalytic activity: ATP + 5'-phosphoribosylformylglycinamide + L-glutamine + H2O = ADP + phosphate + 5'-phosphoribosylformylglycinamidine + L-glutamate]. Probable phosphoribosylformylglycinamidine synthase I PURG (FGAM synthase I) Rv0788, (MTCY369.32), len: 224 aa. Probable purQ, phosphoribosylformylglycinamidine synthase I, equivalent to MLCB5_24|Z95151|O05756|PURQ phosphoribosylformylglycinamidine synthase I from Mycobacterium leprae (224 aa), FASTA scores: opt: 1341, E(): 0, (88.7% identity in 222 aa overlap). Also highly similar to others e.g. P12041|PURQ_BACSU phosphoribosylformylglycinamidine synthase I from Bacillus subtilis (227 aa), FASTA scores: opt: 691, E(): 8.6e-39, (47.7% identity in 214 aa overlap); etc. Contains PS00442 Glutamine amidotransferases class-I active site. Belongs to type-1 glutamine amidotransferases. P9WHL5 No intermediary metabolism and respiration P65902 P9WHL5 GO:0005524,GO:0005737,GO:0006541,GO:0004642,GO:0006189 6.3.5.3 P9WHL5 ML2219,ML2219c MMAR_4897 MSMEG_5831 Mb0812 NC_000962.3 Mycobrowser_v4 CDS 1616961 1617386 . - 0 Rv1439c Rv1439c Unknown Unknown protein Rv1439c, (MTCY493.15), len: 141 aa. Unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06820 No conserved hypotheticals O06820 O06820 O06820 Mb1474c NC_000962.3 Mycobrowser_v4 CDS 4400870 4401634 . + 0 Rv3912 Rv3912 Unknown Hypothetical alanine rich protein Rv3912, (MTV008.03), len: 254 aa. Hypothetical unknown ala-rich protein. Cleaved by Rip|Rv2869c, in M. tuberculosis Erdman (See Sklar et al., 2010). P9WJ65 No conserved hypotheticals P9WJ65 ML2702 MMAR_5476 MSMEG_6932 Mb3943 NC_000962.3 Mycobrowser_v4 tRNA 923803 923875 . - 0 MTB000010 lysT - tRNA-Lys (TTT) lysT, tRNA-Lys, anticodon ttt, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v4 tRNA 923999 924072 . + 0 MTB000011 gluT - tRNA-Glu (TTC) gluT, tRNA-Glu, anticodon ttc, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v4 CDS 884797 885840 . - 0 Rv0791c Rv0791c Function unknown Conserved protein Rv0791c, (MTV042.01c, MTCY369.35c), len: 347 aa. Conserved protein, similar (except in N-terminus) to others e.g. CAC44585.1|AL596162 conserved hypothetical protein from Streptomyces coelicolor (307 aa); NP_252643.1|NC_002516 hypothetical protein from Pseudomonas aeruginosa (364 aa); etc. Also some similarity with oxidoreductases e.g. AAK38097.1|AF323606_3|AF323606 putative F420-dependent dehydrogenase from Rhodococcus erythropolis (295 aa); etc. And also similar in part to other proteins from Mycobacterium tuberculosis e.g. Rv1855c|MTCY359.18|Z83859 (307 aa), FASTA scores: opt: 366, E(): 4e-16, (35.0% identity in 226 aa overlap); Rv3079c|MTCY22D7.02|Z83866 conserved hypothetical protein (275 aa), FASTA scores: opt: 342, E(): 1.2e-14, (31.6% identity in 234 aa overlap); Rv0044c possible oxidoreductase (264 aa). I6X9T8 No conserved hypotheticals P71844 I6X9T8 GO:0016491,GO:0055114 I6X9T8 MMAR_2559 MSMEG_3301 Mb0815c NC_000962.3 Mycobrowser_v4 CDS 885837 886646 . - 0 Rv0792c Rv0792c Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably GntR-family) Rv0792c, (MTV042.02c), len: 269 aa. Probable transcriptional regulator, GntR-family, similar to many others of GntR family e.g. BSUB0018_189|Z99121 from Bacillus subtilis (243 aa), FASTA scores: opt: 367, E(): 1.5e-17, (32.1% identity in 246 aa overlap); P31453|YIDP_ECOLI from Escherichia coli (238 aa), FASTA scores: opt: 236, E(): 8.8e-09, (26.4% identity in 235 aa overlap); etc. O86331 No regulatory proteins O86331 O86331 GO:0006355,GO:0006350,GO:0003700,GO:0005622 O86331 MMAR_2560 MSMEG_4659 Mb0816c NC_000962.3 Mycobrowser_v4 CDS 886719 887024 . + 0 Rv0793 Rv0793 Function unknown Possible monooxygenase Rv0793, (MTV042.03), len: 101 aa. Possible monooxygenase (See Lemieux et al., 2005). Similar to e.g. NP_250888.1|NC_002516 hypothetical protein from Pseudomonas aeruginosa (114 aa); AE 001908|AE001908_7 hypothetical protein from Deinococcus radiodurans (101 aa), FASTA scores: opt: 215, E(): 3.1e-09, (40.4% identity in 99 aa overlap); NP_440966.1|NC_000911|D90908|PCC6803|D90908_2 unknown protein from Synechocystis sp. strain PCC 6803 (147 aa), FASTA scores: opt: 194, E(): 4.5e-08, (31.1% identity in 90 aa overlap); etc. Also similar to Rv2749|MTV002.14|AL0089|MTV002_15 conserved hypothetical protein from Mycobacterium tuberculosis (104 aa), FASTA scores: opt: 143, E(): 0.00026, (26.9% identity in 93 aa overlap). O86332 No intermediary metabolism and respiration 1Y0H O86332 O86332 GO:0005737,GO:0016491,GO:0017000 O86332 MMAR_2561 Mb0817 NC_000962.3 Mycobrowser_v4 CDS 922894 923709 . - 0 Rv0831c Rv0831c Function unknown Conserved protein Rv0831c, (MTV043.23c), len: 271 aa. Conserved protein, similar to Rv0347|MTY13E10_7|Z95324 conserved hypothetical protein from Mycobacterium tuberculosis (328 aa), FASTA scores: opt: 426, E(): 2.6e-21, (33.6% identity in 262 aa overlap). O53842 No conserved hypotheticals O53842 ML2180 Mb0854c NC_000962.3 Mycobrowser_v4 CDS 4118530 4118691 . - 0 Rv3678A Rv3678A Function unknown Conserved hypothetical protein Rv3678A, len: 53 aa. Conserved hypothetical protein, similar to SCH17.10|AL079353_10 conserved hypothetical protein from Streptomyces coelicolor (53 aa), FASTA scores: opt: 259, E(): 1.5e-13, (78.0% identity in 50 aa overlap). I6X824 No conserved hypotheticals I6X824 I6X824 MMAR_5167 MSMEG_6192 Mb3703c NC_000962.3 Mycobrowser_v4 CDS 890388 891482 . + 0 Rv0797 Rv0797 Required for the transposition of the insertion element IS1547. Putative transposase for insertion sequence element IS1547 Rv0797, (MTCI249B.03c, MTV042.07), len: 364 aa. Putative transposase for IS1547; almost identical to (but 20 aa shorter than) Y13470|MTY13470_2 from Mycobacterium tuberculosis (383 aa). Also similar to other transposases e.g. MAIS1110A _1|Q48909 transposase from Mycobacterium avium (464 aa), FASTA scores: opt: 226, E(): 2.4e-08, (30.7% identity in 199 aa overlap). Also slight similarity to Rv2014|MTCY39.03c from Mycobacterium tuberculosis (222 aa), FASTA score: (24.8% identity in 141 aa overlap). O07182 No insertion seqs and phages O07182 O07182 GO:0004803,GO:0006313,GO:0003677 O07182 Mb0820 NC_000962.3 Mycobrowser_v4 CDS 891472 892269 . - 0 Rv0798c cfp29 Unknown. Supposedly released from the envelope to the outside during growth. 29 KDa antigen CFP29 Rv0798c, (MTCI429B.02), len: 265 aa. Cfp29, 29 kDa antigen (see citations below). Highly similar to Q45296|BLLINM18P_1|CAA63787.1|X93588 linocin M18 from Brevibacterium linens (266 aa), FASTA scores: (58.5% identity in 265 aa overlap). Also shows similarity with NP_228594.1|NC_000853 bacteriocin from Thermotoga maritima (262 aa). I6WZG6 No virulence, detoxification, adaptation O07181 I6WZG6 GO:0008233,GO:0042742 I6WZG6 MMAR_4893 MSMEG_5830 Mb0821c NC_000962.3 Mycobrowser_v4 CDS 893318 894619 . + 0 Rv0800 pepC Function unknown; possibly hydrolyzes peptides and/or proteins. Probable aminopeptidase PepC Rv0800, (MTCY07H7A.09c), len: 433 aa. Probable pepC, aminopeptidase I, highly similar (but shorter 17 aa) to Q50022|PEPX aminopeptidase from Mycobacterium leprae (443 aa), FASTA scores: opt: 2237, E(): 0, (78.3% identity in 433 aa overlap). Also highly similar to others from Eukaryotes and bacteria, e.g. T36482 probable aminopeptidase from Streptomyces coelicolor (432 aa), P14904|AMPL_YEAST vacuolar aminopeptidase I precursor from Saccharomyces cerevisiae (514 aa), FASTA scores: opt: 425, E(): 4.8e-21, (31.0% identity in 445 aa overlap); etc. Also similar to hypothetical proteins e.g. P38821|YHR3_YEAST hypothetical 54.2 kDa protein from Saccharomyces cerevisiae (490 aa), FASTA scores: opt: 429, E(): 2.5e-21, (34.8% identity in 443 aa overlap); etc. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P9WHT1 No intermediary metabolism and respiration O06634 P9WHT1 GO:0008237,GO:0006508,GO:0005773,GO:0008270,GO:0004177 3.4.11.- P9WHT1 ML2213,ML2213c MMAR_4891 MSMEG_5828 Mb0823 NC_000962.3 Mycobrowser_v4 CDS 894631 894978 . + 0 Rv0801 Rv0801 Function unknown Conserved protein Rv0801, (MTCY07H7A.08c), len: 115 aa. Conserved protein, similar to many hypothetical proteins from Streptomyces sp. e.g. SCD840A.20|AB81865.1|AL161691 hypothetical protein from Streptomyces coelicolor (145 aa); AF072709|AF072709_8 from Streptomyces lividans (131 aa), FASTA scores: opt: 120, E(): 0.2, (26.3% identity in 118 aa overlap); etc. O06633 No conserved hypotheticals O06633 O06633 MMAR_4890 MSMEG_5827 Mb0824 NC_000962.3 Mycobrowser_v4 CDS 894972 895628 . - 0 Rv0802c Rv0802c Succinylation, substrate unknown Possible succinyltransferase in the GCN5-related N-acetyltransferase family Rv0802c, (MTCY07H7A.07c), len: 218 aa. Possible succinyltransferase in the GNAT (Gcn5-related N-acetyltransferase) family (See Vetting et al., 2008). Shows partial similarity with many acetyltransferases and hypothetical proteins e.g. P96579|BSUB0003_68 probable acetyltransferase from Bacillus subtilis (183 aa), FASTA scores: E(): 0.0044, (26.4% identity in 110 aa overlap). P9WQG7 No intermediary metabolism and respiration 2VZY,2VZZ P9WQG7 GO:0008152,GO:0008080 P9WQG7 ML2212 MMAR_4889 Mb0825c NC_000962.3 Mycobrowser_v4 CDS 1261922 1262158 . + 0 Rv1134 Rv1134 Unknown Hypothetical protein Rv1134, (MTCI65.01), len: 78 aa. Hypothetical unknown protein. O06534 No conserved hypotheticals O06534 Mb1165 NC_000962.3 Mycobrowser_v4 CDS 898081 898710 . + 0 Rv0804 Rv0804 Function unknown Conserved hypothetical protein Rv0804, (MTCY07H7A.05c), len: 209 aa. Conserved hypothetical protein, showing similarity with C-terminus of Rv1863c|MTCY359.10 conserved hypothetical protein from Mycobacterium tuberculosis (256 aa), FASTA scores: opt: 199, E(): 1.2e-05, (33.2% identity in 220 aa overlap); and Rv0658c. Contains PS01151 Fimbrial biogenesis outer membrane usher protein signature. I6Y4V2 No conserved hypotheticals O06630 I6Y4V2 GO:0016020 MMAR_4887 MSMEG_5821 Mb0827 NC_000962.3 Mycobrowser_v4 CDS 898831 899787 . + 0 Rv0805 Rv0805 Hydrolyzes cyclic nucleotide monophosphate to nucleotide monophosphate. Shown to hydrolyze 2',3'-cNMP and 3',5'-cNMP. Class III cyclic nucleotide phosphodiesterase (cNMP PDE) Rv0805, (MTCY07H7A.04c), len: 318 aa. Cyclic nucleotide phosphodiesterase (cNMP PDE) (See Shenoy et al., 2005), member of binuclear metallophosphoesterase superfamily, equivalent to Q50024 from Mycobacterium leprae (317 aa), FASTA scores: opt: 1713, E(): 0, (82.5% identity in 315 aa overlap). Also shows similarity with hypothetical proteins and icc proteins e.g. SC9B1.22c|T35867 hypothetical protein from Streptomyces coelicolor (305 aa); P36650|ICC_ECOLI icc protein from Escherichia coli (275 aa), FASTA scores: opt: 310, E(): 8.9e-14, (31.3% identity in 214 aa overlap); etc. P9WP65 No intermediary metabolism and respiration 2HY1,2HYO,2HYP,3IB7,3IB8 O06629 P9WP65 GO:0016787 P9WP65 ML2210,ML2210c MMAR_4886 Mb0828 NC_000962.3 Mycobrowser_v4 CDS 908181 908483 . - 0 Rv0814c sseC2 Thought to be involved in sulphur metabolism. Conserved protein SseC2 Rv0814c, (MTV043.06c, O05794), len: 100 aa. SseC2, conserved protein, highly similar to AAA62972.1|U15182|MLU15182_17 hypothetical protein from Mycobacterium leprae (143 aa), FASTA scores: opt: 545, E(): 0, (84.0% identity in 100 aa overlap); and NP_302441.1|NC_002677|Z95150|MTCY164_29 conserved hypothetical protein from Mycobacterium leprae (100 aa), FASTA scores: opt: 647, E(): 0, (100.0% identity in 100 aa overlap). Also highly similar to M29612|SERCYSA_5 rhodanese-like protein from Saccharopolyspora erythraea (101 aa), FASTA scores: opt: 345, E(): 1.2e-18, (57.1% identity in 98 aa overlap); and similar at the C-terminus to the C-terminus of CAB94069.1|AL358692 conserved hypothetical protein from Streptomyces coelicolor (95 aa). Identical second copy present as Rv3118|MTCY164.28|SSEC1 from Mycobacterium tuberculosis (100 aa) (100.0% identity in 100 aa overlap). P0CG95 No intermediary metabolism and respiration Q7D986 P0CG95 ML2199 Mb0837c NC_000962.3 Mycobrowser_v4 CDS 916477 917646 . - 0 Rv0823c Rv0823c Thought to be involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0823c, (MTV043.15c), len: 389 aa. Possible transcriptional regulator (resembles nitrogen regulation protein), equivalent (but longer 24 aa in N-terminus) to MLU15182_31|U15182|NtrB NtrB protein from Mycobacterium leprae (384 aa), FASTA scores: opt: 2070, E(): 0, (82.3% identity in 384 aa overlap) (see citation below). Also highly similar to CAB63312.1|AL133471|SCC82.03c hypothetical protein from Streptomyces coelicolor (406 aa); and to many transcriptional regulators members of UPF0034 family (NIFR3/SMM1) e.g. D26185|BAC180K_143 protein similar to transcriptional regulator (nitrogen regulation protein) from Bacillus subtilis (333 aa), FASTA scores: opt: 609, E(): 1.4e-32, (38.3% identity in 326 aa overlap); NP_349795.1|NC_003030 NifR3 family enzyme from Clostridium acetobutylicum (321 aa); etc. Contains PS01136 Uncharacterized protein family UPF0034 signature. P9WNS7 No regulatory proteins O53835 P9WNS7 GO:0055114,GO:0017150,GO:0008033,GO:0050660 1.-.-.- P9WNS7 ML2186 MMAR_4857 MSMEG_5774 Mb0846c NC_000962.3 Mycobrowser_v4 tRNA 924213 924286 . + 0 MTB000013 pheU - tRNA-Phe (GAA) pheU, tRNA-Phe, anticodon gaa, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v4 CDS 901635 902024 . + 0 Rv0807 Rv0807 Function unknown Conserved hypothetical protein Rv0807, (MTCY07H7A.02c), len: 129 aa. Conserved hypothetical protein, equivalent to O05761|MLCB5_31 hypothetical 14.0 kDa protein from Mycobacterium leprae (131 aa), FASTA scores: E(): 0, (73.4% identity in 128 aa overlap). Also highly similar to BAA89438.1|AB003158|ORF3 hypothetical protein from Corynebacterium ammoniagenes (132 aa); and C-terminus of SCD25.20|CAB56364.1|AL118514 hypothetical protein from Streptomyces coelicolor (202 aa). I6Y8U3 No conserved hypotheticals I6Y8U3 I6Y8U3 ML2207,ML2207c MMAR_4882 MSMEG_5817 Mb0830 NC_000962.3 Mycobrowser_v4 CDS 907338 908018 . - 0 Rv0813c Rv0813c Function unknown Conserved protein Rv0813c, (MTV043.05c), len: 226 aa. Conserved protein, highly similar to U15182|MLU15182_16 hypothetical protein from Mycobacterium leprae (242 aa), FASTA scores: opt: 1191, E(): 0, (78.3% identity in 226 aa overlap); and NP_302442.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (228 aa). Also similar to AB94083.1|AL358692|SCD66.16 hypothetical protein from Streptomyces coelicolor (191 aa); and Rv2717c|MTCY05A6_37 hypothetical protein from Mycobacterium tuberculosis (164 aa), FASTA score: (30.4% identity in 171 aa overlap). Possibly a new bacterial family of fatty acid-binding protein-like proteins (See Shepard et al., 2007). P9WFG9 No conserved hypotheticals 2FWV O53827 P9WFG9 GO:0006810 P9WFG9 ML2200 MMAR_4871 MSMEG_5792 Mb0836c NC_000962.3 Mycobrowser_v4 CDS 917734 918750 . - 0 Rv0824c desA1 Catalyzes the principal conversion of saturated fatty acids to unsaturated fatty acids. Thought to convert stearoyl-ACP to oleoyl-ACP by introduction of a cis double bond between carbons delta-9 and delta-10 of the acyl chain [catalytic activity: stearoyl-[acyl-carrier protein] + AH2 + O2 = oleoyl-[acyl-carrier protein] + a + 2 H2O]. Probable acyl-[acyl-carrier protein] desaturase DesA1 (acyl-[ACP] desaturase) (stearoyl-ACP desaturase) (protein Des) Rv0824c, (MTV043.16c), len: 338 aa. Probable desA1 (alternate gene name: des), acyl-[acyl-carrier protein] desaturase (stearoyl-ACP desaturase) (see Jackson et al., 1997), equivalent to U15182|MLU15182_32 acyl-[ACP] desaturase from Mycobacterium leprae (338 aa), FASTA scores: opt: 1880, E(): 0, (79.9% identity in 338 aa overlap); and highly similar in part to fragment CAB96061.1|AJ250019 Steroyl-ACP-desaturase from Mycobacterium avium subsp. paratuberculosis (93 aa). Also similar to other fatty acid desaturases e.g. T35035 probable acyl-[acyl-carrier protein] desaturase from Streptomyces coelicolor (328 aa); Q40731|STAD_ORYSA acyl-[acyl-carrier protein] desaturase precursor from Oryza sativa (Rice) (390 aa); etc. Also highly similar to desA2|Rv1094 from Mycobacterium tuberculosis (275 aa). Contains PS00225 Crystallins beta and gamma 'Greek key' motif signature. Belongs to the fatty acid desaturase family. Cofactor: ferredoxin, ferredoxin NADPH reductase, and NADPH. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WNZ7 No lipid metabolism Q50824 P9WNZ7 GO:0006633,GO:0055114,GO:0046914,GO:0045300 1.14.19.- P9WNZ7 ML2185 MMAR_4856 MSMEG_5773 Mb0847c NC_000962.3 Mycobrowser_v4 CDS 937593 937835 . + 0 Rv0841 Rv0841 Unknown Probable conserved transmembrane protein Rv0841, len: 80 aa. Conserved transmembrane protein, highly similar to C-terminus of next ORF Rv0842|O53854 putative membrane protein from Mycobacterium tuberculosis (442 aa), FASTA scores: opt: 246, E(): 3.3e-10, (59.7% identity in 72 aa overlap). Replace previous Rv0841c. I6WZK3 No cell wall and cell processes I6WZK3 GO:0016021 Mb0864c NC_000962.3 Mycobrowser_v4 CDS 1143736 1144467 . + 0 Rv1022 lpqU Unknown Probable conserved lipoprotein LpqU Rv1022, (MTCY10G2.27c), len: 243 aa. Probable lpqU conserved lipoprotein. Similar to Mycobacterium tuberculosis hypothetical protein Rv1230c|MTV006.02C, FASTA scores: E(): 2.8e-18, (37.9% identity in 240 aa overlap). Similar to AL133423|SC4A7.37 hypothetical protein from Streptomyces coelicolor (421 aa), FASTA scores: opt: 474, E(): 2.7e-21, (42.2% identity in 211 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. P96378 No cell wall and cell processes P96378 P96378 ML0254 MMAR_4463 MSMEG_5416 Mb1050 NC_000962.3 Mycobrowser_v4 CDS 1279998 1280846 . - 0 Rv1153c omt Function unknown, but supposedly involved in lipid metabolism Probable O-methyltransferase Omt Rv1153c, (MTCI65.20c), len: 282 aa. Probable omt, O-methyltransferase, similar to TCMP_STRGA|P39887 Tetracenomycin polyketide synthesis O-methyltransferase tcmP from Streptomyces glaucescens (270 aa), FASTA scores: opt: 368, E(): 1.7e-17, (31.3% identity in 233 aa overlap). O06551 No lipid metabolism O06551 O06551 GO:0008168 2.1.1.- O06551 MMAR_4300 MSMEG_5173 Mb1184c NC_000962.3 Mycobrowser_v4 CDS 1402778 1403386 . - 0 Rv1255c Rv1255c Involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv1255c, (MTCY50.27), len: 202 aa. Possible regulatory protein, similar to others e.g. ACRR_ECOLI|P34000 potential acrab operon repressor from E. coli (215 aa), FASTA scores: opt: 128, E(): 0.25, (42.1% identity in 57 aa overlap). Helix turn helix motif present at aa 36-57 (+5.48 SD). P9WMD5 No regulatory proteins Q11063 P9WMD5 GO:0006350,GO:0003700,GO:0006355 P9WMD5 MMAR_4185 MSMEG_5040 Mb1287c NC_000962.3 Mycobrowser_v4 ncRNA 918264 918458 . + 0 MTB000053 ASdes Unknown Putative small regulatory RNA ASdes, putative small regulatory RNA (See Arnvig and Young, 2009). Alternate 5'-ends at positions 918350 and 918365. Alternate 3'-ends at positions 918432 and 918412. No stable RNAs NC_000962.3 Mycobrowser_v4 CDS 903725 904819 . + 0 Rv0809 purM Involved in de novo purine biosynthesis (at the fifth step) [catalytic activity: ATP + 5'-phosphoribosylformylglycinamidine = ADP + phosphate + 5'-phosphoribosyl-5-aminoimidazole]. Probable phosphoribosylformylglycinamidine CYCLO-ligase PurM (AIRS) (phosphoribosyl-aminoimidazole synthetase) (air synthase) Rv0809, (MTV043.01), len: 364 aa. Probable purM, 5'-phosphoribosyl-5-aminoimidazole synthetase, equivalent to NP_302446.1|NC_002677 5'-phosphoribosyl-5-aminoimidazole synthase from Mycobacterium leprae (364 aa). Also highly similar to many e.g. P12043|PUR5_BACSU phosphoribosylformylglycinamidine CYCLO-ligase from Bacillus subtilis (346 aa), FASTA scores: opt: 1023, E(): 0, (46.5% identity in 331 aa overlap); U68765|STU68765_2 from Salmonella typhimurium (345 aa), FASTA scores: opt: 1014, E():0, (47.6% identity in 330 aa overlap); etc. I6Y4V6 No intermediary metabolism and respiration O53823 I6Y4V6 GO:0005524,GO:0005737,GO:0004641,GO:0006189 6.3.3.1 I6Y4V6 ML2205,ML2205c MMAR_4880 MSMEG_5798 Mb0832 NC_000962.3 Mycobrowser_v4 CDS 904905 905087 . - 0 Rv0810c Rv0810c Function unknown Conserved hypothetical protein Rv0810c, (MTV043.02c), len: 60 aa. Conserved hypothetical protein, with its N-terminus highly similar to NP_302445.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (62 aa); and AL118514|SCD25_24 hypothetical protein from Streptomyces coelicolor (84 aa), FASTA scores: opt: 180, E(): 5.7e-07, (51.8% identity in 56 aa overlap). I6XWB9 No conserved hypotheticals O53824 I6XWB9 ML2204 MMAR_4879 MSMEG_5797 Mb0833c NC_000962.3 Mycobrowser_v4 CDS 905234 906340 . - 0 Rv0811c Rv0811c Function unknown Conserved protein Rv0811c, (MTV043.03c), len: 368 aa. Conserved protein, equivalent to U2266F|U15182|MLU15182_13 hypothetical protein from Mycobacterium leprae (366 aa), FASTA scores: opt: 1870, E(): 0, (77.4% identity in 367 aa overlap). Also highly similar to BAA89441.1|AB003158|ORF4 hypothetical protein from Corynebacterium ammoniagenes (359 aa); and CAB94085.1|AL358692 conserved hypothetical protein from Streptomyces coelicolor (321 aa). I6X9V3 No conserved hypotheticals O53825 I6X9V3 GO:0005737,GO:0006546,GO:0004047 I6X9V3 ML2203 MMAR_4878 MSMEG_5796 Mb0834c NC_000962.3 Mycobrowser_v4 CDS 906423 907292 . + 0 Rv0812 Rv0812 Acts on amino acids. Probable amino acid aminotransferase Rv0812, (MTV043.04), len: 289 aa. Probable amino acid aminotransferase, similar to other amino acid aminotransferases, generelly class-IV of pyridoxal-phosphate-dependent aminotransferases, and especially ILVE proteins and PABC proteins e.g. B76065.1|AL157953 putative aminotransferase from Streptomyces coelicolor (273 aa); NP_069766.1|NC_000917 branched-chain amino acid aminotransferase (ilvE) from Archaeoglobus fulgidus (290 aa); P54692|DAAA_BACLI D-alanine aminotransferase from Bacillus licheniformis (283 aa); P28305|PABC_ECOLI|B1096 4-amino-4-deoxychorismate lyase (ADC lyase) From Escherichia coli strain K12 (269 aa), FASTA scores: opt: 165, E(): 0.00064, (26.8% identity in 198 aa overlap); etc. Note that previously known as pabC. Q79FW0 No intermediary metabolism and respiration Q79FW0 Q79FW0 GO:0008483,GO:0008152 2.6.1.- Q79FW0 ML2202,ML2202c MMAR_4873 MSMEG_5795 Mb0835 NC_000962.3 Mycobrowser_v4 CDS 910030 910842 . - 0 Rv0817c Rv0817c Unknown Probable conserved exported protein Rv0817c, (MTV043.09c), len: 270 aa. Probable conserved exported protein, with N-terminal signal sequence, equivalent (but shorter 13 aa) to U15182|MLU15182_22|U2266M probable exported protein from Mycobacterium leprae (283 aa), FASTA scores: opt: 1287, E(): 0, (73.0% identity in 270 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). I6WZH9 No cell wall and cell processes I6WZH9 ML2195 MMAR_4866 MSMEG_5785 Mb0840c NC_000962.3 Mycobrowser_v4 CDS 1308223 1308792 . - 0 Rv1176c Rv1176c Function unknown Conserved hypothetical protein Rv1176c, (MTV005.12c), len: 189 aa. Conserved hypothetical protein, some similarity to P94443|D78508 hypothetical protein from Bacillus subtilis (182 aa), FASTA scores: opt: 219, E(): 1.7e-15, (25.1% identity in 183 aa overlap). Similar to Mycobacterium tuberculosis hypothetical protein Rv0047c. O50432 No conserved hypotheticals O50432 O50432 O50432 Mb1209c NC_000962.3 Mycobrowser_v4 CDS 911736 912683 . + 0 Rv0819 mshD Involved in the fourth step of mycothiol biosynthesis GCN5-related N-acetyltransferase, MshD Rv0819, (MTV043.11), len: 315 aa. MshD, acetyltransferase involved in mycothiol synthesis (see Koledin et al., 2002). Contains two GNAT (Gcn5-related N-acetyltransferase) domains. See Vetting et al. 2003, 2005, 2006. Equivalent to U2266N|U15182|MLU15182_24 hypothetical protein from Mycobacterium leprae (312 aa), FASTA scores: opt: 1540, E(): 0, (75.2% identity in 314 aa overlap). Also highly similar to CAB88484.1|AL353816 putative acetyltransferase from Streptomyces coelicolor (309 aa). P9WJM7 No intermediary metabolism and respiration 1OZP,1P0H,2C27 O53831 P9WJM7 GO:0008152,GO:0008080 P9WJM7 ML2193,ML2193c MMAR_4864 Mb0842 NC_000962.3 Mycobrowser_v4 CDS 912726 913502 . + 0 Rv0820 phoT Involved in active transport of inorganic phosphate across the membrane (import); responsible for energy coupling to the transport system. This is one of the proteins required for binding-protein-mediated phosphate transport. Probable phosphate-transport ATP-binding protein ABC transporter PhoT Rv0820, (MTV043.12), len: 258 aa. Probable phoT, phosphate-transport ATP-binding protein ABC transporter (see citation below), equivalent to PhoT|MLU15182_28|U15182 phosphate transport system ABC transporter from Mycobacterium leprae (258 aa), FASTA scores: opt: 1556, E(): 0, (91.5% identity in 258 aa overlap). Also highly similar to others e.g. CAB88472.1|AL353816 phosphate ABC transport system ATP-binding protein from Streptomyces coelicolor (258 aa); etc. Note that also highly similar to many PstB proteins e.g. AAC15686.1|AF045938|PstB putative ABC transporter nucleotide binding subunit from Mycobacterium smegmatis (258 aa). Contains PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters). P9WQL1 No cell wall and cell processes O53832 P9WQL1 GO:0005315,GO:0015415,GO:0006817,GO:0005886,GO:0005524 3.6.3.27 P9WQL1 ML2189,ML2189c MMAR_4860 MSMEG_5779 Mb0843 NC_000962.3 Mycobrowser_v4 CDS 918912 919553 . - 0 Rv0825c Rv0825c Function unknown Conserved protein Rv0825c, (MTV043.17c), len: 213 aa. Conserved protein, highly similar, but in part (between aa ~43-96) to fadD27|Rv0275c|MTV035.03 putative fatty-acid-CoA ligase from Mycobacterium tuberculosis (241 aa), FASTA scores: E(): 7.3e-09, (32.6% identity in 190 aa overlap). Also shows similarity with other proteins from Mycobacterium tuberculosis e.g. Rv0078|AL0214|MTV030_22 (201 aa), FASTA scores: opt:118, E(): 0.32, (34.5% identity in 113 aa overlap); etc. O53836 No conserved hypotheticals O53836 O53836 GO:0006350,GO:0003700,GO:0006355 O53836 ML2184 MMAR_4855 MSMEG_5768 Mb0848c NC_000962.3 Mycobrowser_v4 CDS 919634 920689 . + 0 Rv0826 Rv0826 Function unknown Conserved hypothetical protein Rv0826, (MTV043.18), len: 351 aa. Conserved hypothetical protein, similar to CAB94053.1|AL358672|SC7A12.06 hypothetical protein from Streptomyces coelicolor (300 aa); and NP_421372.1|NC_002696 hypothetical protein from Caulobacter crescentus (299 aa). Also similar to other proteins from Mycobacterium tuberculosis e.g. Rv1645c|Z85982|MTCY06H11.09 (351 aa), FASTA scores: opt: 1199, E(): 0, (57.5% identity in 299 aa overlap); Rv2237; Rv0276; etc. O53837 No conserved hypotheticals O53837 O53837 ML2183,ML2183c MMAR_4854 MSMEG_5767 Mb0849 NC_000962.3 Mycobrowser_v4 CDS 920741 921133 . - 0 Rv0827c kmtR Represses transcription from the KMTR operator-promoter. Repression is alleviated by NI(II) or cobalt(II). Metal sensor transcriptional regulator KmtR (ArsR-SmtB family) Rv0827c, (MTV043.19c), len: 130 aa. KmtR, transcriptional regulator (See Campbell et al., 2007), similar to many e.g. CAC42856.1|AL592292 putative regulatory protein from Streptomyces coelicolor (115 aa); NP_301626.1|NC_002677 putative ArsR-family transcriptional regulator from Mycobacterium leprae (140 aa); BSUB0011_75|O31844|Z99114 YOZA protein from Bacillus subtilis (107 aa), FASTA scores: opt: 208, E(): 3.2e-08, (35.5% identity in 93 aa overlap); etc. Also similar to MTCY27.22c|Z95208 from Mycobacterium tuberculosis (135 aa), FASTA scores: opt: 201, E(): 1.2e-07, (35.7% identity in 98 aa overlap). Contains probable helix-turn helix motif from aa 42-63 (Score 1300, +3.61 SD). Belongs to the ArsR family of transcriptional regulators. O53838 No regulatory proteins O53838 O53838 GO:0006355,GO:0006350,GO:0003700,GO:0005622 O53838 ML2182 Mb0850c NC_000962.3 Mycobrowser_v4 CDS 924951 925364 . + 0 Rv0832 PE_PGRS12 Function unknown PE-PGRS family protein PE_PGRS12 Rv0832, (MTV043.24), len: 137 aa. PE_PGRS12, Member of the Mycobacterium tuberculosis PE family, possibly PGRS subfamily of gly-rich proteins (see citation below), highly similar to many others e.g. MTCY1A11.25c|Z78020 (498 aa), FASTA scores: opt: 529, E(): 5.2e-22, (61.8% identity in 136 aa overlap); etc. Appears to have incurred frameshift as next ORF should be continuation; sequence has been checked but no error found. Q79FV8 No PE/PPE Q79FV8 Q79FV8 Q79FV8 Mb0855 NC_000962.3 Mycobrowser_v4 CDS 921191 921613 . - 0 Rv0828c Rv0828c Function unknown; probably involved in deamination of specific substrate. Possible deaminase Rv0828c, (MTV043.20c), len: 140 aa. Possible deaminase, with its N-terminus highly similar to middle part of NP_302602.1|NC_002677 possible cytidine/deoxycytidylate deaminase from Mycobacterium leprae (171 aa). Also similar to other deaminases e.g. CAC18715.2|AL451182 putative deaminase from Streptomyces coelicolor (167 aa); NP_251189.1|NC_002516 probable deaminase from Pseudomonas aeruginosa (151 aa); NP_108387.1|NC_002678 nitrogen fixation protein gene from Mesorhizobium loti (149 aa); etc. Also similar to many conserved hypothetical proteins e.g. NP_389200.1|NC_000964 hypothetical protein from Bacillus subtilis (156 aa), FASTA scores: E(): 1.3e-07, (38.9% identity in 95 aa overlap); etc. And similar to Rv3752c possible deaminase from Mycobacterium tuberculosis. Contains PS00903 Cytidine and deoxycytidylate deaminases zinc-binding region signature. Belongs to the cytidine and deoxycytidylate deaminases family. O53839 No intermediary metabolism and respiration O53839 O53839 GO:0008270,GO:0016787 3.5.-.- O53839 MMAR_4851 Mb0851c NC_000962.3 Mycobrowser_v4 CDS 921575 921865 . + 0 Rv0829 Rv0829 Required for the transposition of the insertion element IS1605'. Possible transposase (fragment) Rv0829, (MTV043.21), len: 96 aa. Possible transposase for IS1605' (fragment), similar to C-terminal end of many mycobacterial transposases and hypothetical proteins e.g. Z74024|MTCY274_16 from Mycobacterium tuberculosis (460 aa), FASTA scores: opt: 668, E(): 6.2e-32, (98.9% identity in 93 aa overlap); MTV002_57|O33333 transposase from Mycobacterium tuberculosis ; L07627|SERRY1_1 insertion element IS1136 from Saccharopolyspora erythraea (90 aa), FASTA score: (34.9% identity in 83 aa overlap). O53840 No insertion seqs and phages O53840 O53840 Mb0852 NC_000962.3 Mycobrowser_v4 CDS 921970 922875 . + 0 Rv0830 Rv0830 Possible methyltransferase Possible S-adenosylmethionine-dependent methyltransferase Rv0830, (MTV043.22), len: 301 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), member of Mycobacterium tuberculosis protein family consisting of the proteins Rv0726c, Rv0731c, Rv3399, Rv1729c|Z81360|MTCY4C12_14c (312 aa), FASTA scores: opt: 1014, E(): 0, (54.1% identity in 292 aa overlap); etc. P9WFI3 No lipid metabolism O53841 P9WFI3 GO:0008168 2.1.1.- P9WFI3 ML2181,ML2181c MMAR_4850 Mb0853 NC_000962.3 Mycobrowser_v4 CDS 939388 940392 . + 0 Rv0843 Rv0843 Function unknown; probably involved in cellular metabolism. Probable dehydrogenase Rv0843, (MTV043.36), len: 334 aa. Probable dehydrogenase, similar to various dehydrogenases e.g. Q46142|Q46142 TPP-dependent acetoin dehydrogenase (326 aa), FASTA scores: opt: 500, E(): 2.4e-26, (32.3% identity in 300 aa overlap); P51267|ODPA_PORPU pyruvate dehydrogenase E1 component from Porphyra purpurea (344 aa), FASTA scores: opt: 451, E(): 4.7e-23, (29.6% identity in 311 aa overlap); etc. Also similar to Rv2497c|pdhA pyruvate dehydrogenase E1 component from Mycobacterium tuberculosis (367 aa). I6XWE5 No intermediary metabolism and respiration O53855 I6XWE5 GO:0016624,GO:0055114 1.-.-.- I6XWE5 MMAR_4784 Mb0866 NC_000962.3 Mycobrowser_v4 CDS 1184883 1185740 . + 0 Rv1062 Rv1062 Function unknown Conserved hypothetical protein Rv1062, (MTV017.15), len: 285 aa. Conserved hypothetical protein, some similarity to AL079356|SC6G9_10 hypothetical protein in Streptomyces coelicolor (289 aa), FASTA scores: opt: 556, E(): 1.2e-27, (39.0% identity in 287 aa overlap), and Z99111|BSUB0008_176 Bacillus subtilis (260aa), FASTA scores: opt: 163, E(): 0.0013, (27.4% identity in 179aa overlap). O53410 No conserved hypotheticals O53410 O53410 GO:0006629 O53410 MMAR_4404 MSMEG_5285 Mb1091 NC_000962.3 Mycobrowser_v4 CDS 934720 935490 . + 0 Rv0838 lpqR Unknown Probable conserved lipoprotein LpqR Rv0838, (MTV043.31), len: 256 aa. Probable lpqR, conserved lipoprotein. Similar (except in N-terminus) to hypothetical proteins and D-alanyl-D-alanine dipeptidases e.g. NP_416005.1|NC_000913 hypothetical protein from Escherichia coli strain K12 (193 aa); NP_421076.1|NC_002696 D-alanyl-D-alanine dipeptidase from Caulobacter crescentus (212 aa); Q06241|VANX_ENTFC D-alanyl-D-alanine dipeptidase from Enterococcus faecium (202 aa), FASTA scores: opt: 198, E(): 1.9e-05, (28.1% identity in 199 aa overlap); etc. Contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O53850 No cell wall and cell processes O53850 O53850 GO:0016805,GO:0008237,GO:0006508,GO:0005618 O53850 MMAR_4800 MSMEG_5879 Mb0861 NC_000962.3 Mycobrowser_v4 CDS 935577 936389 . + 0 Rv0839 Rv0839 Function unknown Conserved hypothetical protein Rv0839, (MTV043.32), len: 270 aa. Conserved hypothetical protein, similar to various hypothetical proteins or methyltransferases from yeast and bacteria e.g. T34740|SC1E6.19c|AL033505|SC1E6_19 hypothetical protein from Streptomyces coelicolor (273 aa), FASTA scores: opt: 1102, E(): 0, (58.6% identity in 263 aa overlap); T38024|Z98598|SPAC1B3.06c hypothetical protein from Schizosaccharomyces pombe (278 aa), FASTA scores: opt: 562, E(): 1.9e-3, (36.4% identity in 269 aa overlap); JC6531 avermectin B 5-O-methyltransferase from Streptomyces avermitilis (283 aa); etc. Also similar to other Mycobacterium tuberculosis hypothetical proteins that may be methyltransferases e.g. Rv1523, Rv2952, Rv1405c, etc. I6X9X6 No conserved hypotheticals O53851 I6X9X6 GO:0008168,GO:0008152 I6X9X6 MMAR_4799 Mb0862 NC_000962.3 Mycobrowser_v4 CDS 936457 937317 . - 0 Rv0840c pip Specifically catalyzes the removal of N-terminal proline residues from peptides. Thought to release the N-terminal proline from the dipeptides, pro-pro, pro-GLN, pro-TRP and pro-TYR; also from amides (pro-beta NA) and oligopeptides, pro-LEU-GLYNH2, pro-LEU-GLY and pro-PHE-GLY-LYS. Higher activity toward small peptides (up to three residues), but very low activity for longer peptides [catalytic activity: release of a N-terminal proline from a peptide]. Probable proline iminopeptidase Pip (prolyl aminopeptidase) (pap) Rv0840c, (MTV043.33c), len: 286 aa. Possible pip, proline iminopeptidase, similar to many e.g. P46541|PIP_BACCO proline iminopeptidase from bacillus coagulans (288 aa), FASTA scores: opt: 657, E(): 0, (37.6% identity in 282 aa overlap); NP_386922.1|NC_003047 putative proline iminopeptidase protein from Sinorhizobium meliloti (296 aa); etc. Belongs to peptidase family S33. I6Y8X0 No intermediary metabolism and respiration O53852 I6Y8X0 GO:0005737,GO:0006508,GO:0004177 3.4.11.5 I6Y8X0 MMAR_4789 MSMEG_2681 Mb0863c NC_000962.3 Mycobrowser_v4 CDS 938112 939404 . + 0 Rv0842 Rv0842 Unknown Probable conserved integral membrane protein Rv0842, (MT0864, MTV043.35), len: 430 aa. Probable conserved integral membrane protein, showing similarity with other integral membrane proteins e.g. P28246|BCR_ECOLI bicyclomycin resistance protein from EScherichia coli (396 aa), FASTA scores: opt: 216, E(): 5.4e-07, (23.7% identity in 376 aa overlap); etc. O53854 No cell wall and cell processes O53854 O53854 GO:0055085 MMAR_4785 Mb0865 NC_000962.3 Mycobrowser_v4 CDS 4373726 4374013 . - 0 Rv3890c esxC Unknown ESAT-6 like protein EsxC (ESAT-6 like protein 11) Rv3890c, (MT4005, MTCY15F10.22), len: 95 aa. EsxC, ESAT-6 like protein (see Gey Van Pittius et al., 2001), equivalent to Q9K548|ES6B_MYCPA putative ESAT-6 like protein 11 (ORF3890C) from Mycobacterium paratuberculosis (95 aa), FASTA scores: opt: 490, E(): 3.3e-26, (76.85% identity in 95 aa overlap). Belongs to the ESAT6 family. P9WNI1 No cell wall and cell processes P9WNI1 ML0034 Mb3919c NC_000962.3 Mycobrowser_v4 CDS 942680 944194 . - 0 Rv0846c Rv0846c May have multicopper oxidase activity. Probable oxidase Rv0846c, (MTV043.39c), len: 504 aa. Probable oxidase, showing similarity with several oxidases, mainly L-ascorbate oxidases and copper resistance proteins a (precursors) e.g. P24792|ASO_CUCMA L-ascorbate oxidase precursor (ascorbase) from Cucurbita maxima (Pumpkin) (Winter squash) (579 aa), FASTA scores: opt: 423, E(): 5.8e-18, (28.4% identity in 493 aa overlap); AF010496|AF010496_32 potential multicopper oxidase from Rhodobacter capsulatus (491 aa), FASTA scores: opt: 490, E(): 2.7e-22, (28.8% identity in 510 aa overlap); 47452|PCOA_ECOLI copper resistance protein A precursor (belongs to the family of multicopper oxidases) from Escherichia coli strain K12 (605 aa); etc. Contains PS00080 Multicopper oxidases signature 2 at C-terminus. Seems to belong to the family of multicopper oxidases. I6WZK7 No intermediary metabolism and respiration O53858 I6WZK7 GO:0055114,GO:0016491,GO:0005507 1.-.-.- I6WZK7 ML2171 MMAR_4770 Mb0869c NC_000962.3 Mycobrowser_v4 CDS 944343 944735 . + 0 Rv0847 lpqS Unknown Probable lipoprotein LpqS Rv0847, (MTV043.40), len: 130 aa. Probable lpqS, lipoprotein. Contains possible signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O53859 No cell wall and cell processes O53859 MMAR_4769 Mb0870 NC_000962.3 Mycobrowser_v4 CDS 948559 949395 . + 0 Rv0852 fadD16 Function unknown, but involved in lipid degradation. Possible fatty-acid-CoA ligase FadD16 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv0852, (MTV043.45), len: 278 aa. Possible fadD16, fatty-acid-CoA synthetase, similar in part to various CoA ligases e.g. P18163|LCFB_RAT long-chain-fatty-acid--CoA ligase from Rattus norvegicus (Rat) (699 aa); D49366|LEP4CCOALA_1 4-coumarate:CoA ligase from Lithospermum erythrorhizon (636 aa), FASTA scores: opt: 134, E(): 0.15, (26.8% identity in 213 aa overlap); orgp|L09229|HUMFACAL_1 long-chain acyl-coenzyme A from homo sapiens (human) (699 aa), FASTA score: (50.0% identity in 40 aa overlap); etc. Contains PS00626 Regulator of chromosome condensation (RCC1) signature 2. I6Y4Z4 No lipid metabolism I6Y4Z4 GO:0016874 6.2.1.- I6Y4Z4 ML2168,ML2168c MMAR_4685 MSMEG_3566 Mb0875 NC_000962.3 Mycobrowser_v4 CDS 951183 951626 . + 0 Rv0854 Rv0854 Function unknown Conserved protein Rv0854, (MTV043.47), len: 147 aa. Conserved protein, similar to several hypothetical protein from Mycobacterium leprae e.g. NP_301674.1|NC_002677 (144 aa); NP_302683.1|NC_002677|Z95398|MLCL622.27c (156 aa), FASTA scores: opt: 193, E(): 1.6e-06, (24.6% identity in 134 aa overlap); NP_301218.1|NC_002677 (146 aa); MTCI28.04|Z97050 (184 aa), FASTA scores: opt: 171, E(): 5.8e-05, (21.5% identity in 135 aa overlap). Also similar to SC6G10.02c|T35511|AL049497|SC6G10_2 hypothetical protein from Streptomyces coelicolor (144 aa), FASTA scores: opt: 344, E(): 6.1e- 17, (37.6% identity in 141 aa overlap). And similar to many proteins from Mycobacterium tuberculosis e.g. downstreams ORFs Rv0856 and Rv0857, etc. I6X9Y7 No conserved hypotheticals O53866 I6X9Y7 I6X9Y7 ML2166,ML2166c MMAR_4682 MSMEG_5736 Mb0877 NC_000962.3 Mycobrowser_v4 CDS 1008944 1010062 . + 0 Rv0906 Rv0906 Function unknown Conserved protein Rv0906, (MTCY31.34), len: 372 aa. Conserved protein, highly similar to others e.g. SC6A5.25|AL049485|T35416 hypothetical protein from Streptomyces coelicolor (370 aa), FASTA scores: opt: 1125, E(): 0, (51.3% identity in 335 aa overlap); NP_242955.1|NC_002570|BH2089 conserved protein from Bacillus halodurans (370 aa); etc. Also shows some similarity to C-terminus of Q48412|ROMA_KLEPN Q48412 outer membrane protein roma (fragment) from Klebsiella pneumoniae (132 aa), FASTA scores: opt: 319, E(): 8.5e-14, (46.2% identity in 104 aa overlap); NP_105215.1|NC_002678 hypothetical protein which contains similarity to outer membrane protein romA from Enterobacter cloacae (350 aa); etc. Predicted to be an outer membrane protein (See Song et al., 2008). P9WKP3 No conserved hypotheticals P9WKP3 P9WKP3 ML2117,ML2117c MMAR_4624 MSMEG_5638 Mb0930 NC_000962.3 Mycobrowser_v4 CDS 1002441 1002704 . - 0 Rv0898c Rv0898c Function unknown Conserved hypothetical protein Rv0898c, (MTCY31.26c), len: 87 aa. Conserved hypothetical protein, highly similar to CAC01589.1|AL391041 hypothetical protein from Streptomyces coelicolor (87 aa). Also shows some similarity to Rv0709|MTCY210.28|rpmC from Mycobacterium tuberculosis (77 aa), FASTA score: (28.8% identity in 73 aa overlap). P9WKP5 No conserved hypotheticals P64753 P9WKP5 MMAR_4638 MSMEG_5666 Mb0922c NC_000962.3 Mycobrowser_v4 CDS 946056 947315 . + 0 Rv0849 Rv0849 Thought to be involved in transport of undetermined substrate (possibly drug) across the membrane. Responsible for the translocation of the substrate across the membrane. Probable conserved integral membrane transport protein Rv0849, (MTV043.42), len: 419 aa. Probable conserved integral membrane transport protein, possibly member of major facilitator superfamily (MFS) involved in transport of drug, showing similarity with others e.g. T35055 probable transport system permease protein from Streptomyces coelicolor (436 aa); NP_295031.1|NC_001263 major facilitator family protein from Deinococcus radiodurans (458 aa); NP_455659.1|NC_003198 putative membrane transporter from Salmonella enterica subsp. enterica serovar Typhi (402 aa); etc. P9WJX5 No cell wall and cell processes O53861 P9WJX5 GO:0005886,GO:0055085,GO:0016021 MMAR_4767 Mb0872 NC_000962.3 Mycobrowser_v4 CDS 947312 947644 . + 0 Rv0850 Rv0850 Required for the transposition of an insertion element. Putative transposase (fragment) Rv0850, (MTV043.43), len: 110 aa. Putative transposase (fragment), similar in part to others e.g. Q45144|Q4514 transposable element IS31831 (436 aa), FASTA scores: opt: 175, E(): 4.3e-05, (38.6% identity in 57 aa overlap); etc. I6Y8X8 No insertion seqs and phages I6Y8X8 Mb0873 NC_000962.3 Mycobrowser_v4 CDS 949436 951118 . - 0 Rv0853c pdc Possible indole-3-pyruvate decarboxylase; EC 4.1.1.74 [catalytic activity: 3-(indol-3-YL)pyruvate = 2-(indol-3-YL)acetaldehyde + CO2], or possible pyruvate decarboxylase; EC 4.1.1.1 [catalytic activity: a 2-oxo acid = an aldehyde + CO2]. Probable pyruvate or indole-3-pyruvate decarboxylase Pdc Rv0853c, (MTV043.46c), len: 560 aa. Probable pdc, pyruvate or indole-pyruvate decarboxylase, equivalent to NP_302424.1|NC_002677 pyruvate (or indolepyruvate) decarboxylase from Mycobacterium leprae (569 aa). Also highly similar to others e.g. AAB06571.1|L80006 indolepyruvate decarboxylase from Pantoea agglomerans (550 aa); Q12629|DCPY_KLULA pyruvate decarboxylase from Kluyveromyces marxianus var. lactis (563 aa); P71323 indolepyruvate decarboxylase from Enterobacter herbicola (550 aa), FASTA scores: opt: 1642, E(): 0, (48.1% identity in 547 aa overlap); P23234|DCIP_ENTCL indole-3-pyruvate decarboxylase (indolepyruvate decarboxylase) from Enterobacter cloacae (552 aa), FASTA scores: opt: 1596, E(): 0, (46.8% identity in 551 aa overlap); etc. Contains PS00187 Thiamine pyrophosphate enzymes signature and PS00017 ATP/GTP-binding site motif A (P-loop). Cofactor: thiamine pyrophosphate. P9WG37 No intermediary metabolism and respiration O53865 P9WG37 GO:0000287,GO:0030976,GO:0016740,GO:0016831 4.1.1.- P9WG37 ML2167 MMAR_4683 MSMEG_5735 Mb0876c NC_000962.3 Mycobrowser_v4 CDS 972546 973706 . - 0 Rv0874c Rv0874c Function unknown Conserved hypothetical protein Rv0874c, (MTCY31.02c), len: 386 aa. Conserved hypothetical protein, highly similar in part to SPU62616_1 hypothetical protein from Synechococcus sp. (280 aa), FASTA scores: E(): 6.3e-26, (35.2% identity in 264 aa overlap); SYCSLLLH_102 from Synechocystis sp. (447 aa), FASTA scores: E(): 1.1e-18, (29.5% identity in 400 aa overlap). Also highly similar to Rv0628c|MTCY20H10_9 from Mycobacterium tuberculosis (383 aa), FASTA scores: E():0, (81.5% identity in 383 aa overlap). P9WKR9 No conserved hypotheticals P0A5D3 P9WKR9 GO:0005886,GO:0016021 P9WKR9 Mb0898c NC_000962.3 Mycobrowser_v4 CDS 952825 953229 . + 0 Rv0856 Rv0856 Function unknown Conserved hypothetical protein Rv0856, (MTV043.49), len: 134 aa. Conserved hypothetical protein, showing weak similarity with NP_301674.1| (NC_002677) conserved hypothetical protein from Mycobacterium leprae (144 aa); and SC6G10.02c|T35511 hypothetical protein from Streptomyces coelicolor (144 aa). Also highly similar to other proteins from Mycobacterium tuberculosis e.g. neighbouring ORF downstream Rv0857 conserved hypothetical protein (126 aa), FASTA scores: E(): 7.4e-27, (62.0% identity in 100 aa overlap); neighbouring ORF Rv0854|MTV043_47 conserved hypothetical protein (147 aa), FASTA scores: E(): 1.6e-15, (36.6% identity in 123 aa overlap), MTCI28.04|Z97050|MTCI28_4 (184 aa), FASTA scores: opt: 127, E(): 0.036, (26.0% identity in 127 aa overlap); and MLCL622.27c|Z95398 (156 aa), FASTA scores: opt: 123, E(): 0.06, (26.4% identity in 125 aa overlap). O53868 No conserved hypotheticals O53868 O53868 O53868 Mb0879 NC_000962.3 Mycobrowser_v4 CDS 953257 953730 . + 0 Rv0857 Rv0857 Function unknown Conserved hypothetical protein Rv0857, (MTV043.50), len: 157 aa. Conserved hypothetical protein, showing weak similarity with Q9X7Y8|SC6G10.02c|T35511 hypothetical protein from Streptomyces coelicolor (144 aa), FASTA scores: opt: 215, E(): 7.6e-08, (30.282% identity in 142 aa overlap). Also highly similar to other proteins from Mycobacterium tuberculosis e.g. upstream ORF Rv0856 (134 aa), FASTA scores: opt: 566, E(): 2e-32, (58.15% identity in 129 aa overlap); upstream ORF Rv0854 (147 aa), FASTA scores: opt: 401, E(): 7.2e-21, (41.8% identity in 146 aa overlap); MTCI28.04|Z97050 (184 aa), FASTA scores: opt: 122, E(): 0.031, (29.4% identity in 85 aa overlap); and MLCL622.27c|Z95398 (156 aa), FASTA scores: opt: 114, E(): 0.1, (30.9% identity in 55 aa overlap). Length extended since first submission (+33 aa). I6Y4Z9 No conserved hypotheticals O53869 I6Y4Z9 I6Y4Z9 ML2164,ML2164c MMAR_4679 Mb0880 NC_000962.3 Mycobrowser_v4 CDS 953727 954920 . - 0 Rv0858c dapC Involved in biosynthesis of diaminopimelate and lysine from aspartate semialdehyde (at the fourth step) [catalytic activity: L-glutamate + N-succinyl-2-amino-6-ketopimelate = 2-oxoglutarate + N-succinyl-L,L-2,6-diaminopimelate Probable N-succinyldiaminopimelate aminotransferase DapC (DAP-at) Rv0858c, (MTV043.51c), len: 397 aa. Probable dapC, N-succinyldiaminopimelate aminotransferase, highly similar to others from Eukaryota and bacteria, especially aspartate aminotransferases (transaminases), e.g. NP_177890.1|NC_003070 putative aminotransferase from Arabidopsis thaliana (440 aa); NP_419555.1|NC_002696 aminotransferase class I from Caulobacter crescentus (385 aa); NP_415133.1|NC_000913|AE0001|ECAE000165_8 putative aminotransferase from Escherichia coli strain K12 (386 aa), FASTA scores: opt: 830, E(): 0, (38.0% identity in 389 aa overlap); X99521|TAX99521_1 aspartate aminotransferase from Thermus aquaticus (383 aa), FASTA scores: opt: 702, E(): 0, (34.9% identity in 393 aa overlap); etc. Also similar to other putative aminotransferases from Mycobacterium tuberculosis e.g. Rv2294, Rv3565, etc. P9WPZ5 No intermediary metabolism and respiration 2O0R O53870 P9WPZ5 GO:0009058,GO:0030170,GO:0008483,GO:0016847 2.6.1.- P9WPZ5 ML2163 MMAR_4678 MSMEG_5725 Mb0881c NC_000962.3 Mycobrowser_v4 CDS 1145858 1146544 . + 0 Rv1024 Rv1024 Unknown Possible conserved membrane protein Rv1024, (MTCY10G2.25c), len: 228 aa. Possible conserved membrane protein, with a hydrophobic region from aa 83-101. Equivalent to ML0256|NP_301311.1|NC_002677 possible conserved membrane protein from Mycobacterium leprae (227 aa), S&W scores: 178, E()= 2e-72, Identities: 145/203 (71%). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P96376 No cell wall and cell processes P96376 P96376 GO:0051301,GO:0016021,GO:0005886,GO:0007049 ML0256 MMAR_4461 Mb1052 NC_000962.3 Mycobrowser_v4 CDS 1417658 1418356 . - 0 Rv1268c Rv1268c Unknown Hypothetical protein Rv1268c, (MTCY50.14), len: 232 aa. Hypothetical unknown protein, probably secreted protein : contains possible signal peptide sequence (score 7.9 at residue 28). Predicted to be an outer membrane protein (See Song et al., 2008). P9WM47 No conserved hypotheticals P9WM47 GO:0006508,GO:0004197 P9WM47 MMAR_4154 Mb1299c NC_000962.3 Mycobrowser_v4 CDS 967344 967733 . - 0 Rv0870c Rv0870c Unknown Possible conserved integral membrane protein Rv0870c, (MTV043.63c), len: 129 aa. Possible conserved integral membrane protein, highly similar to other membrane proteins: putative secreted proteins or hypothetical proteins e.g. CAC08263.1| AL392146 putative integral membrane protein from Streptomyces coelicolor (138 aa); NP_233433.1|NC_002506 conserved hypothetical protein from Vibrio cholerae (143 aa); NP_455572.1|NC_003198 putative membrane protein from Salmonella enterica subsp. enterica serovar Typhi (148 aa); P37065|YCCF_ECOLI hypothetical 16.3 kDa protein from Escherichia coli (148 aa), FASTA scores: opt: 183, E(): 1.9e-06, (36.6% identity in 134 aa overlap); etc. I6Y8Z3 No cell wall and cell processes O53882 I6Y8Z3 ML2148 MMAR_4662 MSMEG_5697 Mb0894c NC_000962.3 Mycobrowser_v4 CDS 967898 968305 . + 0 Rv0871 cspB Function unknown; thought to act in response to low temperature. Probable cold shock-like protein B CspB Rv0871, (MTV043.64), len: 135 aa. Probable cspB, cold shock-like protein B, equivalent to Z99494|MLCB57_7|MLCB57.11 probable cold shock protein from Mycobacterium leprae (136 aa), FASTA scores: opt: 787, E(): 0, (86.0% identity in 136 aa overlap). Also highly similar (but often longer than) to others e.g. CAB93399.1|AL357524 cold shock protein B from Streptomyces coelicolor (127 aa); Q45099|CSPD_BACCE cold shock-like protein CSPD from Bacillus cereus (66 aa); Y101 81|LLCSPB_1 cold shock protein from Lactococcus lactis (66 aa), FASTA scores: opt: 220, E(): 2.5e-07, (48.3% identity in 60 aa overlap); etc. Seems to belong to the cold-shock domain (CSD) family. I6WZM9 No virulence, detoxification, adaptation O53883 I6WZM9 GO:0005737,GO:0006355,GO:0003677 I6WZM9 ML2147,ML2147c MMAR_4661 MSMEG_5696 Mb0895 NC_000962.3 Mycobrowser_v4 CDS 973806 974294 . - 0 Rv0875c Rv0875c Unknown Possible conserved exported protein Rv0875c, (MTCY31.03c), len: 162 aa. Possible conserved exported protein, equivalent to MLCB57_11|O33056 possible exported protein from Mycobacterium leprae (162 aa), FASTA scores: opt: 789, E(): 0, (71.4% identity in 161 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). P9WKR7 No cell wall and cell processes P9WKR7 ML2144 MMAR_4657 MSMEG_5694 Mb0899c NC_000962.3 Mycobrowser_v4 CDS 1138967 1142671 . + 0 Rv1020 mfd Involved in nucleotide excision repair. Necessary for strand-specific repair. A lesion in the template strand blocks the RNA polymerase complex (RNAP). The RNAP-DNA-RNA complex is specifically recognized by TRCF which releases RNAP and the truncated transcript; the TCRF may replace RNAP at the lesion site and then recruit the UVRA/B/C repair system. Probable transcription-repair coupling factor Mfd (TRCF) Rv1020, (MTCY10G2.29c), len: 1234 aa. Probable mfd (alternate gene name: trcF), transcription-repair coupling factor (see citation below), similar to many e.g. MFD_ECOLI|P30958 transcription-repair coupling factor from Escherichia coli (1148 aa), FASTA scores: opt: 1900, E(): 0, (37.9% identity in 1107 aa overlap); similar to M. tuberculosis Rv2973c and Rv1633. Contains PS00017 ATP/GTP-binding site motif A (P-loop). In the N-terminal section; belongs to the UVRB family. In the C-terminal section; belongs to the helicase family. RECG subfamily. P9WMQ5 No information pathways P64326 P9WMQ5 GO:0008026,GO:0006281,GO:0003684,GO:0006355,GO:0003700,GO:0005524 3.6.4.- P9WMQ5 ML0252 MMAR_4465 MSMEG_5423 Mb1048 NC_000962.3 Mycobrowser_v4 CDS 2340871 2341815 . + 0 Rv2083 Rv2083 Unknown Conserved hypothetical protein Rv2083, (MTCY49.22), len: 314 aa. Conserved hypothetical protein. Similar to many e.g. Mycobacterium tuberculosis Rv3898c (110 aa) and Rv3897c (210 aa). P9WLK3 No conserved hypotheticals P9WLK3 GO:0005886,GO:0016021 Mb2109 NC_000962.3 Mycobrowser_v4 CDS 960342 962612 . - 0 Rv0862c Rv0862c Function unknown Conserved protein Rv0862c, (MTV043.55c), len: 756 aa. Conserved protein, equivalent to NP_302419.1|NC_002677 possible DNA-binding protein from Mycobacterium leprae (753 aa); and highly similar (except in C-terminus) to MLCB57.01|Z99494|T45333 hypothetical protein from Mycobacterium leprae (>577 aa, truncated), FASTA scores: opt: 3047, E(): 0, (78.9% identity in 578 aa overlap). Also similar in part to SCD12A.03c|AB93395.1|AL357524 hypothetical protein from Streptomyces coelicolor (867 aa). O53874 No conserved hypotheticals O53874 O53874 ML2156 MMAR_4670 MSMEG_5705 Mb0885c NC_000962.3 Mycobrowser_v4 CDS 962599 962880 . + 0 Rv0863 Rv0863 Function unknown Conserved hypothetical protein Rv0863, (MTV043.56), len: 93 aa. Conserved hypothetical protein, highly similar to NP_302418.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (74 aa). Also weakly similar in part to U82598|ECU82598_135 hypothetical protein from Escherichia coli, FASTA scores: (32.4% identity in 71 aa overlap); and M74011|YEPYSCOP_8 hypothetical protein from Yersinia enterocolitica (165 aa), FASTA scores: (38.6 identity in 57 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). I6XWF9 No conserved hypotheticals I6XWF9 I6XWF9 ML2155,ML2155c MMAR_4669 MSMEG_5704 Mb0886 NC_000962.3 Mycobrowser_v4 CDS 974291 975937 . - 0 Rv0876c Rv0876c Unknown Possible conserved transmembrane protein Rv0876c, (MTCY31.04c), len: 548 aa. Possible conserved transmembrane protein, equivalent to MLCB57_12|O33057 possible membrane protein from Mycobacterium leprae (579 aa), FASTA scores: opt: 2850, E(): 0, (81.0% identity in 568 aa overlap). Also highly similar (except in N-terminus) to CAB93403.1|AL357524 putative integral membrane protein from Streptomyces coelicolor (463 aa). P9WKR5 No cell wall and cell processes Q10564 P9WKR5 GO:0005886,GO:0055085,GO:0016021 ML2143 MMAR_4656 MSMEG_5693 Mb0900c NC_000962.3 Mycobrowser_v4 CDS 978934 979365 . + 0 Rv0880 Rv0880 Thought to be involved in transcriptional mechanism. Possible transcriptional regulatory protein (possibly MarR-family) Rv0880, (MTCY31.08), len: 143 aa. Possible transcriptional regulator, MarR family, equivalent to MLCB57_15|O3306|NP_302411.1|NC_002677 putative MarR-family protein from Mycobacterium leprae (143 aa), FASTA scores: opt: 818, E(): 0, (89.5% identity in 143 aa overlap). Also similar to many others e.g. CAB93410.1|AL357524 putative marR-family protein from Streptomyces coelicolor (145 aa); NP_251757.1|NC_002516 probable transcriptional regulator from Pseudomonas aeruginosa (147 aa); etc. Also similar to Rv2327 from Mycobacterium tuberculosis (163 aa). P9WMF1 No regulatory proteins P67745 P9WMF1 GO:0006355,GO:0006350,GO:0003700,GO:0005622 P9WMF1 ML2140,ML2140c Mb0904 NC_000962.3 Mycobrowser_v4 CDS 964312 965535 . - 0 Rv0867c rpfA Unknown. May be promote the resuscitation and growth of dormant, nongrowing cell. Possible resuscitation-promoting factor RpfA Rv0867c, (MTV043.60c), len: 407 aa. Possible rpfA, resuscitation-promoting factor (see citation below). N-terminus highly similar to N-terminal part (1-125 aa) of Z99494|MLCB57_3|NP_302417.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (174 aa), FASTA scores: opt: 785, E(): 1.8e-18, (63.0% identity in 200 aa overlap); and highly similar to C-terminus of NP_301299.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (375 aa); and middle part of NP_302360.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (157 aa). N-terminus also highly similar in part of three secreted proteins from Streptomyces coelicolor e.g. CAC09538.1|AL442120 putative secreted protein (244 aa). Regions highly similar to CAB76321.1|AL158060 putative membrane protein from Streptomyces coelicolor (121 aa); and middle part of CAB09664.1|Z96935 rpf from Micrococcus luteus (220 aa). Also highly similar in part to four resuscitation-promoting factors from Mycobacterium tuberculosis: Rv2450 (172 aa), Rv1009 (362 aa), Rv1884c (176 aa), and Rv2389c (154 aa). Contains a probable secretory signal sequence in N-terminus. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WG31 No cell wall and cell processes O53879 P9WG31 GO:0005576 P9WG31 ML2151 MMAR_4665 MSMEG_5700 Mb0891c NC_000962.3 Mycobrowser_v4 CDS 965983 966261 . - 0 Rv0868c moaD2 Involved in molybdenum cofactor biosynthesis. Probable molybdenum cofactor biosynthesis protein D 2 MoaD2 (molybdopterin converting factor small subunit) (molybdopterin [MPT] converting factor, subunit 1) Rv0868c, (MTV043.61c), len: 92 aa. Probable moaD2, molybdenum cofactor biosynthesis protein (molybdopterin converting factor (subunit 1)), similar to CAB88494.1|AL353816 putative molybdopterin converting factor from Streptomyces coelicolor (84 aa); and weakly similar to others MoaD proteins e.g. Z99111|BSUB0008_103 from Bacillus subtilis (77 aa), FASTA scores: opt: 86, E(): 2.8, (22.9% identity in 83 aa overlap); etc. Also some similarity with Rv3112|MOAD1|MTCY164.22 putative molybdenum cofactor biosynthesis protein D from Mycobacterium tuberculosis (83 aa), FASTA scores: opt: 113, E(): 0.024, (31.3% identity in 83 aa overlap). I6XWG2 No intermediary metabolism and respiration O53880 I6XWG2 GO:0006790 I6XWG2 ML2150 MMAR_4664 MSMEG_5699 Mb0892c NC_000962.3 Mycobrowser_v4 CDS 1418579 1418953 . - 0 Rv1269c Rv1269c Unknown Conserved probable secreted protein Rv1269c, (MTCY50.13), len: 124 aa. Conserved probable exported protein with putative N-terminal signal sequence. Similar to Mycobacterium tuberculosis protein Rv1813c|Y0DU_MYCTU|Q50620 hypothetical protein cy1a11.30 (137 aa), FASTA scores: E(): 9e-21, (41.6% identity in 137 aa overlap). P9WM45 No cell wall and cell processes P9WM45 MMAR_4153 Mb1300c NC_000962.3 Mycobrowser_v4 CDS 968424 970244 . - 0 Rv0872c PE_PGRS15 Function unknown PE-PGRS family protein PE_PGRS15 Rv0872c, (MTV043.65c), len: 606 aa. PE_PGRS15, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002), similar to many e.g. MTCY24A1.04c|Z95207 (615 aa), FASTA scores: opt: 2636, E(): 0, (64.6% identity in 619 aa overlap); etc. Q79FV3 No PE/PPE Q79FV3 Q79FV3 Q79FV3 Mb0896c NC_000962.3 Mycobrowser_v4 CDS 970505 972457 . + 0 Rv0873 fadE10 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE10 Rv0873, (MTV043.66-MTCY31.01), len: 650 aa. Probable fadE10, acyl-CoA dehydrogenase, highly similar to many e.g. CAB91129.1|AL355913 putative acyl CoA dehydrogenase from Streptomyces coelicolor (658 aa); P50544|ACDV_MOUSE acyl-CoA dehydrogenase from Mus musculus (656 aa); D30647|RATVLCAD_1 very-long-chain Acyl-CoA dehydrogenase from Rattus norvegicus (655 aa), FASTA scores: opt: 675, E(): 0, (33.9% identity in 380 aa overlap); etc. P9WQF7 No lipid metabolism P63429 P9WQF7 GO:0003995,GO:0055114,GO:0050660 P9WQF7 ML2146,ML2146c MMAR_4660 Mb0897 NC_000962.3 Mycobrowser_v4 CDS 976075 976863 . + 0 Rv0877 Rv0877 Function unknown Conserved hypothetical protein Rv0877, (MTCY31.05), len: 262 aa. Conserved hypothetical protein, equivalent to MLCB57_13|O33058 conserved hypothetical protein from Mycobacterium leprae (269 aa), FASTA scores: E(): 0, (80.5% identity in 257 aa overlap). Also highly similar (except in C-terminus) to SCD12A.13|CAB93404.1|AL357524 hypothetical protein from Streptomyces coelicolor (308 aa). P9WKR3 No conserved hypotheticals P64733 P9WKR3 ML2142,ML2142c MMAR_4655 MSMEG_5691 Mb0901 NC_000962.3 Mycobrowser_v4 CDS 978481 978756 . - 0 Rv0879c Rv0879c Unknown Possible conserved transmembrane protein Rv0879c, (MTCY31.07c), len: 91 aa. Possible conserved transmembrane protein, C-terminus highly similar to C-terminal part of MLCB57_14|O33059 conserved hypothetical protein from Mycobacterium leprae (91 aa), FASTA scores: E(): 1.2e-25, (76.9% identity in 91 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WKR1 No cell wall and cell processes P64735 P9WKR1 GO:0005886,GO:0016021 ML2141 MMAR_4653 MSMEG_5689 Mb0903c NC_000962.3 Mycobrowser_v4 CDS 1426164 1427417 . + 0 Rv1277 Rv1277 Function unknown Conserved hypothetical protein Rv1277, (MTCY50.05c), len: 417 aa. Conserved hypothetical protein, some similarity to 3914967|O68033|SBCD_RHOCA exonuclease SBCD homolog from Rhodobacter capsulatus (405 aa). May be sbcD protein (see Mizrahi & Andersen 1998) Q50699 No conserved hypotheticals Q50699 Q50699 GO:0004527 Q50699 ML1119 MMAR_4143 MSMEG_5004 Mb1308 NC_000962.3 Mycobrowser_v4 CDS 3127364 3128272 . - 0 Rv2820c Rv2820c Unknown Hypothetical protein Rv2820c, (MTCY16B7.22), len: 302 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WJF7 No conserved hypotheticals P9WJF7 P9WJF7 Mb2844c NC_000962.3 Mycobrowser_v4 CDS 980225 980509 . + 0 Rv0882 Rv0882 Unknown Probable transmembrane protein Rv0882, (MTCY31.10), len: 94 aa. Probable transmembrane protein. P9WKQ9 No cell wall and cell processes P9WKQ9 GO:0005886,GO:0016021 ML2138,ML2138c MMAR_4650 MSMEG_5686 Mb0906 NC_000962.3 Mycobrowser_v4 CDS 980506 981267 . - 0 Rv0883c Rv0883c Function unknown Conserved hypothetical protein Rv0883c, (MTCY31.11c), len: 253 aa. Conserved hypothetical protein, equivalent to O3306|MLCB57_16 conserved hypothetical protein from Mycobacterium leprae (251 aa), FASTA scores: E(): 0, (79.4% identity in 253 aa overlap). Also highly similar to N_terminus of AL009204|SC9B10_22 hypothetical protein from Streptomyces coelicolor (352 aa), FASTA scores: E(): 6.1e-20, (35.0% identity in 246 aa overlap). P9WKQ7 No conserved hypotheticals P64739 P9WKQ7 P9WKQ7 ML2137 MMAR_4649 MSMEG_5685 Mb0907c NC_000962.3 Mycobrowser_v4 CDS 982762 983784 . + 0 Rv0885 Rv0885 Function unknown Conserved hypothetical protein Rv0885, (MTCY31.13), len: 340 aa. Conserved hypothetical protein, equivalent to O33063|MLCB57_18 possible transmembrane protein from Mycobacterium leprae (341 aa), FASTA score: (83.9% identity in 341 aa overlap). Also similar except in C-terminus to T35630 probable membrane protein from Streptomyces coelicolor (312 aa). P9WKQ5 No conserved hypotheticals P9WKQ5 GO:0005886,GO:0016021 P9WKQ5 ML2135,ML2135c MMAR_4647 MSMEG_5682 Mb0909 NC_000962.3 Mycobrowser_v4 CDS 983803 985530 . + 0 Rv0886 fprB Serves as the first electron transfer protein in all the P450 systems [catalytic activity: reduced adrenodoxin + NADP+ = oxidized adrenodoxin + NADPH]. Probable NADPH:adrenodoxin oxidoreductase FprB (adrenodoxin reductase) (AR) (ferredoxin-NADP(+) reductase) Rv0886, (MTCY31.14), len: 575 aa. Probable fprB, ferredoxin/ferredoxin-NADP(+) reductase (NADPH:adrenodoxin oxidoreductase), equivalent to O3306|MLCB57_19 ferredoxin/ferredoxin--NADP reductase from Mycobacterium leprae (555 aa), FASTA scores: E(): 0, (76.6 identity in 560 aa overlap). Also highly similar or similar to others e.g. NP_294219.1|NC_001263 putative ferredoxin/ferredoxin--NADP reductase from Deinococcus radiodurans (479 aa) (N-terminus shorter); P22570|ADRO_HUMAN NADPH:adrenodoxin oxidoreductase from homo sapiens (497 aa), FASTA scores: opt: 624, E(): 3e-30, (39.7% identity in 484 aa overlap); P08165|ADRO_BOVIN NADPH:adrenodoxin oxidoreductase from Bos taurus (492 aa); etc. Also similar to others from Mycobacterium tuberculosis e.g. Rv3106, Rv3858c, etc. Contains PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature. P9WJI1 No intermediary metabolism and respiration P65528 P9WJI1 GO:0009055,GO:0022900,GO:0004324,GO:0046872,GO:0006810,GO:0051539 1.18.1.2 P9WJI1 ML2134,ML2134c MMAR_4646 MSMEG_5681 Mb0910 NC_000962.3 Mycobrowser_v4 CDS 987233 988705 . + 0 Rv0888 Rv0888 Unknown Probable exported protein Rv0888, (MTCY31.16), len: 490 aa. Probable exported protein. Equivalent to AAK45157.1 from Mycobacterium tuberculosis strain CDC1551 (507 aa) but shorter 17 aa. Contains possible N-terminal signal sequence. Predicted to be an outer membrane protein (See Song et al., 2008). P9WKQ1 No cell wall and cell processes P64743 P9WKQ1 GO:0005886,GO:0016021 P9WKQ1 MMAR_4644 Mb0912 NC_000962.3 Mycobrowser_v4 CDS 989948 992596 . - 0 Rv0890c Rv0890c Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably LuxR-family) Rv0890c, (MTCY31.18c), len: 882 aa. Probable transcriptional regulatory protein, LuxR family, highly similar (but shorter 238 aa in N-terminus) to NP_302202.1|NC_002677 possible transcriptional regulator from Mycobacterium leprae (1106 aa). Also highly similar (generally in part) to others e.g. T50568 probable multi-domain regulatory protein from Streptomyces coelicolor (1334 aa); P10957|NARL_ECOLI nitrate/nitrite response regulator protein from Escherichia coli (216 aa), FASTA scores: opt: 193, E(): 6e-06, (37.4% identity in 99 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. MTCY02B10_22, MTV008_44, MTV036_21, and MTCY31_24. Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00622 Bacterial regulatory proteins, luxR family signature, and probable helix-turn helix motif from aa 836 to 857 (Score 1559, +4.50 SD). Belongs to the LuxR/UhpA family of transcriptional regulators. Alternative nucleotide at position 990001 (G->C; P866A) has been observed. P9WMG1 No regulatory proteins Q10550 P9WMG1 GO:0005622,GO:0006355,GO:0043565,GO:0006350,GO:0003700,GO:0000156,GO:0000160,GO:0006952 P9WMG1 Mb0914c NC_000962.3 Mycobrowser_v4 CDS 992598 993455 . - 0 Rv0891c Rv0891c Thought to be involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0891c, (MTCY31.19c), len: 285 aa. Possible transcriptional regulator, highly similar in N-terminus to NP_302202.1|NC_002677 possible transcriptional regulator from Mycobacterium leprae (1106 aa). Also highly similar to several Mycobacterium tuberculosis putative transcriptional regulators e.g. Q1102|MTCY02B10_22 probable transcriptional regulatory protein (1159 aa), FASTA scores: opt: 702, E(): 8.3e-40, (50.6% identity in 247 aa overlap); MTV036_21; MTV008_44; MTCY02B10_23. Also shows similarity with several adenylate cyclases and hydrolases from other organisms. P9WMV1 No regulatory proteins Q10551 P9WMV1 GO:0016849,GO:0009190 P9WMV1 Mb0915c NC_000962.3 Mycobrowser_v4 CDS 993853 995340 . + 0 Rv0892 Rv0892 Function unknown; probably involved in cellular metabolism. Probable monooxygenase Rv0892, (MTCY31.20), len: 495 aa. Probable monooxygenase, highly similar to others e.g. NP_250787.1|NC_002516 probable flavin-binding monooxygenase from Pseudomonas aeruginosa (491 aa); CAB59668.1|AL132674 monooxygenase from Streptomyces coelicolor (519 aa); P12015|CYMO_ACIS cyclohexanone monooxygenase from Acinetobacter sp. (542 aa), FASTA scores: opt: 489, E(): 6.8e-26, (30.3% identity in 492 aa overlap); etc. Also highly similar to Rv0565c, Rv3854c, Rv3083, etc from Mycobacterium tuberculosis. Has hydrophobic stretch at N-terminus. P9WNG1 No intermediary metabolism and respiration P64745 P9WNG1 GO:0050661,GO:0004499,GO:0055114,GO:0050660 1.14.13.- P9WNG1 MMAR_4641 Mb0916 NC_000962.3 Mycobrowser_v4 CDS 995318 996295 . - 0 Rv0893c Rv0893c Possible methyltransferase Possible S-adenosylmethionine-dependent methyltransferase Rv0893c, (MTCY31.21c), len: 325 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), belongs in family with P96823|Rv0146|MTCI5.20 from Mycobacterium tuberculosis (310 aa), FASTA scores: opt: 784, E(): 0, (43.2% identity in 308 aa overlap); Rv0726c, Rv0731c, Rv3399, etc. Also shows some similarity with others e.g. SC9B5.10|T35930 hypothetical protein from Streptomyces coelicolor (303 aa); BSUB0008_141|Q45500 hypothetical 34.8 kDa protein from Bacillus subtilis (304 aa), FASTA scores: E(): 0.00033, (26.8% identity in 168 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WFI1 No lipid metabolism P64747 P9WFI1 GO:0008168 2.1.1.- P9WFI1 Mb0917c NC_000962.3 Mycobrowser_v4 CDS 1016236 1016685 . + 0 Rv0912 Rv0912 Unknown Probable conserved transmembrane protein Rv0912, (MTCY21C12.06), len: 149 aa. Probable conserved transmembrane protein, equivalent to Q50121|NP_302397.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (144 aa), FASTA scores: opt: 677, E(): 6.9e-38, (69.5% identity in 141 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O05904 No cell wall and cell processes O05904 GO:0016021 ML2111,ML2111c MMAR_4616 MSMEG_5613 Mb0936 NC_000962.3 Mycobrowser_v4 CDS 1024211 1024687 . + 0 Rv0918 Rv0918 Function unknown Conserved protein Rv0918, (MTCY21C12.12), len: 158 aa. Conserved protein, similar in part to Q50116 hypothetical protein from Mycobacterium leprae (44 aa), FASTA scores: opt: 132, E(): 0.0055, (65.6% identity in 32 aa overlap). Also some similarity in C-terminus with other hypothetical proteins e.g. NP_289961.1|NC_002655 hypothetical protein from Escherichia coli strain O157:H7 (94 aa); etc. O05910 No conserved hypotheticals O05910 O05910 ML2108,ML2108c Mb0942 NC_000962.3 Mycobrowser_v4 CDS 1158918 1159307 . - 0 Rv1034c Rv1034c Possibly required for the transposition of the insertion element IS1560. Probable transposase (fragment) Rv1034c, (MTCY10G2.15), len: 129 aa. Probable IS1560 transposase fragment, similar to part of Rv3387|E1202305|MTV004.45 (225 aa) (65.1% identity in 129 aa overlap). I6X043 No insertion seqs and phages I6X043 Mb1063c NC_000962.3 Mycobrowser_v4 CDS 997782 999299 . + 0 Rv0895 Rv0895 May be involved in synthesis of triacylglycerol Possible triacylglycerol synthase (diacylglycerol acyltransferase) Rv0895, (MTCY31.23), len: 505 aa. Possible triacylglycerol synthase (See Daniel et al., 2004); member of family with: Rv3740c, Rv3734c, Rv1425, Rv1760, etc. Shows some similarity with NP_301898.1|NC_002677 conserved membrane protein from Mycobacterium leprae (491 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WKA3 No lipid metabolism P67204 P9WKA3 GO:0006071,GO:0008610,GO:0004144 2.3.1.20 P9WKA3 Mb0919 NC_000962.3 Mycobrowser_v4 CDS 999472 1000767 . + 0 Rv0896 gltA2 Involved in tricarboxylic acid cycle (KREBS cycle) [catalytic activity: citrate + CoA = acetyl-CoA + H2O + oxaloacetate]. Probable citrate synthase I GltA2 Rv0896, (MTCY31.24), len: 431 aa. Probable gltA2, citrate synthase 1, highly similar to O33066|NP_302405.1|NC_002677 citrate synthase 1 from Mycobacterium leprae (431 aa), FASTA scores: E(): 0, (91.0 identity in 431 aa overlap); and AAF04133.1|AF191033_1|AF191033 citrate synthase from Mycobacterium smegmatis (441 aa). Also highly similar to others e.g. AAF14286.1|AF181118_1|AF181118 citrate synthase from Streptomyces coelicolor (429 aa); P42457|CISY_CORGL citrate synthase from Corynebacterium glutamicum (437 aa), FASTA scores: opt: 1847, E(): 0, (63.0% identity in 433 aa overlap); etc. Also similar to two other Mycobacterium tuberculosis citrate synthases, Rv0889|MTCY31.17c|citA (373 aa), FASTA score: (29.2% identity in 274 aa overlap) and Rv1131|MTCY22G8.20|gltA1 (393 aa). Contains PS00480 Citrate synthase signature. Belongs to the citrate synthase family. P9WPD5 No intermediary metabolism and respiration Q10530 P9WPD5 GO:0004108,GO:0005737,GO:0006099,GO:0044262 2.3.3.1 P9WPD5 ML2130,ML2130c MMAR_4640 MSMEG_5672 Mb0920 NC_000962.3 Mycobrowser_v4 CDS 1000808 1002415 . - 0 Rv0897c Rv0897c Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv0897c, (MTCY31.25c), len: 535 aa. Possible oxidoreductase, similar to various oxidoreductases from diverse organisms e.g. CAB94055.1|AL358672 putative oxidoreductase from Streptomyces coelicolor (540 aa); NP_147877.1|NC_000854 phytoene dehydrogenase from Aeropyrum pernix (549 aa); Q01671|CRTD_RHOSH methoxyneurosporene dehydrogenase from Rhodobacter sphaeroides (495 aa), FASTA scores: opt: 139, E(): 2.6e-06, (23.8% identity in 538 aa overlap); etc. Also similar to Rv1432, Rv2997, and Rv3829c from Mycobacterium tuberculosis. P9WKP7 No intermediary metabolism and respiration P9WKP7 GO:0005886,GO:0016021 ML2127 MMAR_4639 MSMEG_5667 Mb0921c NC_000962.3 Mycobrowser_v4 CDS 1027685 1029337 . + 0 Rv0922 Rv0922 Required for the transposition of the insertion element IS1535 Possible transposase Rv0922, (MTCY21C12.16), len: 550 aa. Possible transposase for IS1535, similar to many e.g. YX16_MYCTU|Q10809|MTCY274.16c from Mycobacterium tuberculosis (460 aa), FASTA scores: opt 939, E(): 0, (40.6% identity in 465 aa overlap); etc. I6Y560 No insertion seqs and phages O05914 I6Y560 I6Y560 Mb0946 NC_000962.3 Mycobrowser_v4 CDS 1076196 1076678 . - 0 Rv0964c Rv0964c Unknown Hypothetical protein Rv0964c, (MTCY10D7.10), len: 160 aa. Hypothetical unknown protein. Equivalent to AAK45241.1 from Mycobacterium tuberculosis strain CDC1551 (138 aa) but longer 22 aa. P9WKM5 No conserved hypotheticals P9WKM5 Mb0989c NC_000962.3 Mycobrowser_v4 CDS 1142671 1143648 . + 0 Rv1021 Rv1021 Function unknown Conserved protein Rv1021, (MTCY10G2.28c), len: 325 aa. Conserved protein, similar to YBL1_STRCI|P33653 hypothetical 26.1 kDa protein from Streptomyces cacaoi (242 aa), FASTA scores: opt: 493, E(): 1.1e-23, (42.9% identity in 238 aa overlap). P96379 No conserved hypotheticals P96379 P96379 P96379 ML0253 MMAR_4464 MSMEG_5422 Mb1049 NC_000962.3 Mycobrowser_v4 CDS 1005852 1006562 . - 0 Rv0903c prrA Transcriptional regulator part of the two component regulatory system PRRA/PRRB. Thought to be involved in the environmental adaptation, specifically in an early phase of the intracellular growth. Two component response transcriptional regulatory protein PrrA Rv0903c, (MTCY31.31c), len: 236 aa. PrrA, two-component response regulator (see citations below), equivalent to Z99494|MLCB57_27|NP_302402.1|NC_002677 two-component response regulator from Mycobacterium leprae (233 aa), FASTA scores: opt: 1414, E(): 0, (95.7% identity in 233 aa overlap); and similar to T45446 probable two-component response regulator from Mycobacterium leprae (253 aa). Also similar to many sensor-like histidine kinase proteins e.g. CAB88489.1|AL353816 putative two-component systen response regulator from Streptomyces coelicolor (248 aa); AAG36759.1|AF119221_1 |AF119221 response regulator from Corynebacterium glutamicum (232 aa); Q02540|COPR_PSESM transcriptional activator protein COPR from Pseudomonas syringae (pv. tomato) (227 aa), FASTA scores: opt: 600, E(): 0, (44.4% identity in 225 aa overlap); etc. Also similar to Rv0981 from Mycobacterium tuberculosis (230 aa), Rv3765c (234 aa), phoP (247 aa), etc. Thought to be induced at phagocytosis (see Graham & Clark-Curtiss 1999). P9WGM1 No regulatory proteins 1YS6,1YS7 P0A5Z6 P9WGM1 GO:0005737,GO:0006355,GO:0006350,GO:0000156,GO:0000160,GO:0003677 P9WGM1 ML2123 MMAR_4633 MSMEG_5662 Mb0927c NC_000962.3 Mycobrowser_v4 CDS 1010136 1011734 . + 0 Rv0907 Rv0907 Function unknown, possibly involved in cell wall biosynthesis. Conserved protein Rv0907, (MTCY21C12.01), len: 532 aa. Conserved protein, possibly involved in cell wall biosynthesis: similar to many beta-lactamases, penicillin-binding proteins and hypothetical proteins e.g. NP_298910.1|NC_002488 beta-lactamase from Xylella fastidiosa (455 aa); Q06317|PBP4_NOCLA penicillin-binding protein 4 (PBP-4) (381 aa), FASTA scores: opt: 299, E(): 8.8e-05, (28.7% identity in 401 aa overlap); etc. N-terminus highly similar to AAA63047.1|U15184 hypothetical protein from Mycobacterium leprae (58 aa). Related to other putative esterases and penicillin binding proteins in Mycobacterium tuberculosis e.g. Rv1730c|MTCY04C12.15c (517 aa). O05900 No cell wall and cell processes O05900 O05900 O05900 ML2116,ML2116c MMAR_4623 MSMEG_5637 Mb0931 NC_000962.3 Mycobrowser_v4 CDS 1017217 1018725 . - 0 Rv0913c Rv0913c Function unknown; probably involved in cellular metabolism. Possible dioxygenase Rv0913c, (MTCY21C12.07c), len: 502 aa. Possible dioxygenase, showing similarity with others e.g. AAK38744.1|AY029525 carotenoid 9,10-9',10' cleavage dioxygenase from Phaseolus vulgaris (543 aa); CAB56138.1|AL117669 putative dioxygenase from Streptomyces coelicolor (503 aa); AAK06796.1|AF324838_15|AF324838 putative dioxygenase SimC5 from Streptomyces antibioticus (456 aa); Q53353|S65040 lignostilbene-alpha,beta-dioxygenase from Pseudomonas paucimobilis (485 aa), FASTA scores: opt: 310, E(): 3.4e-20, (28.9% identity in 495 aa overlap); etc. Also some similarity with Rv0654|MTCI376.22 probable dioxygenase from Mycobacterium tuberculosis (501 aa). I6Y551 No intermediary metabolism and respiration O05905 I6Y551 GO:0016702,GO:0055114 1.-.-.- I6Y551 ML2110 MMAR_4614 MSMEG_5609 Mb0937c NC_000962.3 Mycobrowser_v4 CDS 1008207 1008938 . + 0 Rv0905 echA6 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Possible enoyl-CoA hydratase EchA6 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv0905, (MTCY31.33), len: 243 aa. Possible echA6, enoyl-CoA hydratase, highly similar to ML15184|U15184 enoyl-CoA hydratase from Mycobacterium leprae (247 aa), FASTA score: (85.8% identity in 247 aa overlap). Also similar to many e.g. NP_250320.1|NC_002516 probable enoyl-CoA hydratase/isomerase from Pseudomonas aeruginosa (261 aa); NP_415911.1|NC_000913 putative enzyme from Escherichia coli strain K12 (255 aa); P24162|ECHH_RHOCA|FADB1 enoyl-CoA hydratase homolog from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (257 aa), FASTA scores: opt: 404, E():7.8e-21, (37.3% identity in 249 aa overlap); etc. P9WNP1 No lipid metabolism 3HE2 P64014 P9WNP1 GO:0006631,GO:0004300 4.2.1.17 P9WNP1 ML2118,ML2118c MMAR_4625 MSMEG_5639 Mb0929 NC_000962.3 Mycobrowser_v4 CDS 1014681 1014860 . + 0 Rv0909 Rv0909 Function unknown Conserved hypothetical protein Rv0909, (MTCY21C12.03), len: 59 aa. Conserved hypothetical protein, equivalent to NP_302399.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (56 aa). Also some similarity with AL022268|SC4H2_10c hypothetical protein from Streptomyces coelicolor (97 aa), FASTA scores: opt: 106, E(): 0.13, (43.2% identity in 37 aa overlap). P9WJ07 No conserved hypotheticals P9WJ07 ML2114,ML2114c MMAR_4620 MSMEG_5635 Mb0933 NC_000962.3 Mycobrowser_v4 CDS 1014866 1015300 . + 0 Rv0910 Rv0910 Function unknown Conserved hypothetical protein Rv0910, (MTCY21C12.04), len: 144 aa. Conserved hypothetical protein, equivalent to NP_302398.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (181 aa), FASTA scores: opt: 820, E(): 0, (83.9% identity in 143 aa overlap). Also similar to Rv1546|MTCY48.19c hypothetical protein from Mycobacterium tuberculosis (143 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WJ05 No conserved hypotheticals O05902 P9WJ05 P9WJ05 ML2113,ML2113c MMAR_4619 MSMEG_5634 Mb0934 NC_000962.3 Mycobrowser_v4 CDS 1015398 1016171 . + 0 Rv0911 Rv0911 Function unknown Conserved protein Rv0911, (MTCY21C12.05), len: 257 aa. Conserved protein, showing similarity with hydroxylases and hypothetical proteins e.g. T35325 probable hydroxylase from Streptomyces coelicolor (265 aa); Q54242 hypothetical protein from Streptomyces, FASTA scores: opt: 372, E(): 8.8e-18, (32.0% identity in 256 aa overlap); AAD04716.1|U77891 doxorubicin biosynthesis enzyme DnrV from Streptomyces peucetius (275 aa); AAA63051.1|U15184 hypothetical protein from Mycobacterium leprae (94 aa); etc. Also similar to Rv0577 hypothetical protein from Mycobacterium tuberculosis (261 aa). I6XA34 No conserved hypotheticals O05903 I6XA34 I6XA34 ML2112,ML2112c MMAR_4617 MSMEG_5616 Mb0935 NC_000962.3 Mycobrowser_v4 CDS 1020058 1021329 . - 0 Rv0915c PPE14 Function unknown. Possibly a protective antigen involved with the early control of infection. PPE family protein PPE14 Rv0915c, (MTCY21C12.09c), len: 423 aa. PPE14 (alternate gene name: MTB41). Member of the Mycobacterium tuberculosis PPE family (see citation below), highly similar to many e.g. Rv1807 from Mycobacterium tuberculosis (403 aa), FASTA scores: opt: 966, E(): 4.4e-30, (45.7% identity in 392 aa overlap); etc. Contains PS00626 Regulator of chromosome condensation (RCC1) signature 2. P9WI33 No PE/PPE Q79FV1 P9WI33 P9WI33 Mb0939c NC_000962.3 Mycobrowser_v4 CDS 1021344 1021643 . - 0 Rv0916c PE7 Function unknown PE family protein PE7 Rv0916c, (MTCY21C12.10c), len: 99 aa. PE7 (alternate gene name: MTB10). Member of the Mycobacterium tuberculosis PE family (see citations below), similar to many e.g. Rv1788 from Mycobacterium tuberculosis (99 aa), FASTA scores: opt: 321, E(): 1.3e-11, (53.5% identity in 99 aa overlap); etc. I6Y936 No PE/PPE Q7D937 I6Y936 I6Y936 Mb0940c NC_000962.3 Mycobrowser_v4 CDS 1064114 1064962 . - 0 Rv0953c Rv0953c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0953c, (MTCY10D7.21), len: 282 aa. Possible oxidoreductase, equivalent to CAA48222.1|X68102 hypothetical protein from Mycobacterium avium subsp. paratuberculosis (166 aa). Similar to several hypothetical proteins and oxidoreductases e.g. AAK38097.1|AF323606_3|AF323606 putative F420-dependent dehydrogenase from Rhodococcus erythropolis (295 aa); NP_070025.1|NC_000917 N5,N10-methylenetetrahydromethanopterin reductase (mer-2) from Archaeoglobus fulgidus (348 aa); etc. Also similar to several hypothetical proteins and oxidoreductases from Mycobacterium tuberculosis e.g. Rv2161c|O06216|Z95388|MTCY270.07 (288 aa), FASTA scores: opt: 633, E(): 0, (40.4% identity in 277 aa overlap), Rv3079c (275 aa), Rv0791c (347 aa), etc. Contains PS00201 Flavodoxin signature. P9WKN5 No intermediary metabolism and respiration P64769 P9WKN5 P9WKN5 ML0157,ML0157c MMAR_4546 MSMEG_5520 Mb0978c NC_000962.3 Mycobrowser_v4 CDS 1077233 1077835 . - 0 Rv0966c Rv0966c Function unknown Conserved protein Rv0966c, (MTCY10D7.08), len: 200 aa. Conserved protein, equivalent to AL035500|MLCL373_12 conserved hypothetical protein from Mycobacterium leprae (200 aa), FASTA scores: opt: 1080, E(): 0, (79.5% identity in 200 aa overlap). Also highly similar to SCE6.30c|CAB88834.1|AL353832 hypothetical protein from Streptomyces coelicolor (277 aa). Some similarity to Rv2862c|MTV007.08 conserved hypothetical protein from Mycobacterium tuberculosis (194 aa), FASTA scores: E(): 3.1e-06, (31.5% identity in 184 aa overlap). Equivalent to AAK45243.1 from Mycobacterium tuberculosis strain CDC1551 (230 aa) but shorter 30 aa. Note that Rv0966c has been shortened since first entry. P9WKM1 No conserved hypotheticals P71544 P9WKM1 P9WKM1 ML0169 MMAR_4539 MSMEG_5505 Mb0991c NC_000962.3 Mycobrowser_v4 CDS 2204866 2205273 . - 0 Rv1962c vapC35 Unknown Possible toxin VapC35. Contains PIN domain. Rv1962c, (MTCY09F9.02), len: 135 aa. Possible vapC35, toxin, part of toxin-antitoxin (TA) operon with Rv1962A, contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. Rv3408|MTCY78.20c (133 aa) (36.2% identity in 138 aa overlap); and Rv3384c (130 aa) (43.1% identity in 130 aa overlap) P9WF67 No virulence, detoxification, adaptation P95252 P9WF67 P9WF67 Mb1997c NC_000962.3 Mycobrowser_v4 CDS 1031896 1032633 . - 0 Rv0925c Rv0925c Function unknown Conserved protein Rv0925c, (MTCY21C12.19c), len: 245 aa. Conserved protein, similar to AL132991|SCF55_19 hypothetical protein from Streptomyces coelicolor (197 aa), FASTA scores: opt: 459, E(): 1.2e-23, (39.3% identity in 201 aa overlap). I6Y946 No conserved hypotheticals O05917 I6Y946 I6Y946 MMAR_4583 Mb0948c NC_000962.3 Mycobrowser_v4 CDS 1032710 1033786 . - 0 Rv0926c Rv0926c Function unknown Conserved hypothetical protein Rv0926c, (MTCY21C12.20c), len: 358 aa. Conserved hypothetical protein, similar to Rv1059 conserved hypothetical protein from Mycobacterium tuberculosis (354 aa). Also shows some similarity to AF170923|AF170923_3 dihydrodipicolinate reductase from Mastigocladus laminosus (278 aa), FASTA scores: opt: 170, E(): 0.00088, (25.7% identity in 276 aa overlap). I6WZS8 No conserved hypotheticals O05918 I6WZS8 GO:0008839,GO:0009089,GO:0055114 ML2097 MMAR_4582 MSMEG_5586 Mb0949c NC_000962.3 Mycobrowser_v4 CDS 1033840 1034631 . - 0 Rv0927c Rv0927c Function unknown; possibly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv0927c, (MTCY21C12.21c), len: 263 aa. Probable short-chain dehydrogenase/reductase, similar to various dehydrogenases/reductases, notably 7-alpha-hydroxysteroid dehydrogenases and glucose 1-dehydrogenases e.g. P25529|HDHA_ECOLI 7-alpha-hydroxysteroid dehydrogenase from Escherichia coli (255 aa), FASTA scores: opt: 551, E(): 1e-26, (39.5% identity in 248 aa overlap); NP_252778.1|NC_002516 probable short-chain dehydrogenase from Pseudomonas aeruginosa (253 aa); AAC44307.1|U59433 3-ketoacyl-acyl carrier protein reductase from Bacillus subtilis (246 aa); etc. Also similar to other dehydrogenases from Mycobacterium tuberculosis e.g. MTCY09F9.36, E():1.4e-18; MTCY369.14, E():8e-17; MTCY02B10.14, E():2.5e-14; MTCY09F9.23c, E():1.5e-13; MTCY03C7.07, E():1.9e-13. Contains PS00061 Short-chain dehydrogenases/reductases family signature, and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the short-chain dehydrogenases/reductases (SDR) family. P9WGQ5 No intermediary metabolism and respiration O05919 P9WGQ5 GO:0055114,GO:0016491 1.-.-.- P9WGQ5 ML2096 MMAR_4581 MSMEG_5584 Mb0950c NC_000962.3 Mycobrowser_v4 CDS 1034903 1036015 . + 0 Rv0928 pstS3 Involved in active transport of inorganic phosphate across the membrane (import). This is one of the proteins required for binding-protein-mediated phosphate transport. Periplasmic phosphate-binding lipoprotein PstS3 (PBP-3) (PstS3) (PHOS1) Rv0928, (MTCY21C12.22), len: 370 aa. PstS3 (previously known as phoS2), phosphate-binding lipoprotein component of inorganic phosphate transport system (see citations below), highly similar to others from Mycobacterium leprae e.g. Q50099|PSTS3|PHOS1 phosphate-binding protein 3 precursor (328 aa), FASTA scores: opt: 1772, E(): 0, (79.6% identity in 328 aa overlap); and highly similar to others e.g. AAF74819.1|AF137360_1|AF137360 periplasmic phosphate permease from Mycobacterium avium (369 aa). Also highly similar to Rv0932c|MTCY08D9.07|pstS2 phosphate-binding periplasmic lipoprotein (370 aa); and Rv0934|pstS1 phosphate-binding periplasmic lipoprotein (374 aa) from Mycobacterium tuberculosis (Mycobacterium tuberculosis seems to have three PstS-like proteins, others being Rv0932c and Rv0934c). Contains lipoprotein signature (PS00013) at N-terminus. Belongs to family of phosphate receptors for bacterial ABC-type lipoprotein transporters. P9WGT7 No cell wall and cell processes P0A5Y2 P9WGT7 GO:0006817,GO:0005886,GO:0006950,GO:0005315 P9WGT7 ML2095,ML2095c MMAR_4580 Mb0951 NC_000962.3 Mycobrowser_v4 CDS 1053765 1054241 . + 0 Rv0944 Rv0944 This enzyme may play a significant role in processes leading to recovery from mutagenesis and/or cell death by alkylating agents [catalytic activity: hydrolysis of DNA containing ring-opened N7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimide]. Possible formamidopyrimidine-DNA glycosylase (FAPY-DNA glycosylase) Rv0944, (MTCY10D7.30c), len: 158 aa. Possible formamidopyrimidine-DNA glycosylase, similar to C-terminus of formamidopyrimidine-DNA glycosylases e.g. CAB63194.1|AL133469 putative formamidopyrimidine-DNA glycosylase from Streptomyces coelicolor (287 aa); FPG_LACLA|NP_266509.1|NC_002662 formamidopyrimidine-DNA glycosylase from Lactococcus lactis subsp. lactis (273 aa), FASTA scores: opt: 246, E(): 2.4e-09, (28.9% identity in 142 aa overlap); O50606|FPG_THETH|MUTM|FPG formamidopyrimidine-DNA glycosylase from Thermus thermophilus (267 aa); etc. Also similar to C-termini of endonucleases or DNA glycosylases of Mycobacterium tuberculosis e.g. Rv3297, Rv2464c, Rv2924c. May belong to the FPG family. L0T864 No information pathways P71565 L0T864 GO:0006289,GO:0008534,GO:0008270,GO:0003684 3.2.2.23 L0T864 ML0148 Mb0969 NC_000962.3 Mycobrowser_v4 CDS 1036999 1037925 . + 0 Rv0930 pstA1 Involved in active transport of inorganic phosphate across the membrane (import); responsible for the translocation of the substrate across the membrane. This is one of the proteins required for binding-protein-mediated phosphate transport. Probable phosphate-transport integral membrane ABC transporter PstA1 Rv0930, (MTCY21C12.24), len: 308 aa. Probable pstA1, phosphate-transport integral membrane ABC transporter (see citation below), highly similar to others e.g. NP_302393.1|NC_002677 membrane-bound component of phosphate transport from Mycobacterium leprae (304 aa); CAB88473.1|AL353816 phosphate ABC transport system permease protein from Streptomyces coelicolor (354 aa) (N-terminus longer); NP_312689.1|NC_002695 phosphate transport system permease protein PstA from Escherichia coli strain O157:H7 (296 aa); etc. Also similar to Rv0936|MTCY08D9.03c|PSTA2 probable transmembrane ABC transporter component of phosphate uptake system from Mycobacterium tuberculosis (301 aa). P9WG11 No cell wall and cell processes O86345 P9WG11 GO:0016021,GO:0006817,GO:0005886,GO:0005315 P9WG11 ML2093,ML2093c Mb0953 NC_000962.3 Mycobrowser_v4 CDS 1037920 1039914 . - 0 Rv0931c pknD Involved in signal transduction (via phosphorylation). Thought to regulate phosphate transport. Can phosphorylate the peptide substrate myelin basic protein (MBP) at serine and threonine residues. Can be autophosphorylated on threonine residues [catalytic activity: ATP + a protein = ADP + a phosphoprotein]. Transmembrane serine/threonine-protein kinase D PknD (protein kinase D) (STPK D) Rv0931c, (MTCY08D9.08), len: 664 aa. PknD (alternate gene name: mbk), transmembrane serine/threonine protein kinase (see citations below), equivalent to CAB62227.1|AJ250200 putative serine/threonine protein kinase from Mycobacterium bovis BCG (291 aa); and highly similar in N-terminus to P54744|PKNB_MYCLE probable serine/threonine-specific protein kinase from Mycobacterium leprae (622 aa). Also highly similar to others, particularly in N-terminal half e.g. NP_243370.1|NC_002570 serine/threonine protein kinase from Bacillus halodurans (664 aa); NP_268044.1|NC_002662 serine/threonine protein kinase from Lactococcus lactis (627 aa); etc. Also highly similar to other serine/threonine protein kinases from Mycobacterium tuberculosis e.g. pknH (626 aa), FASTA scores: opt: 1398, E: 0, (49.3% identity in 540 aa overlap); pknE (566 aa); pknB (626 aa); Rv3524 (343 aa); etc. Contains Hank's kinase subdomain. Contains two transmembrane segments, which flank a highly repetitive region, suggesting a receptor-like anchoring. Belongs to the Ser/Thr family of protein kinases. Experimental studies show evidence of auto-phosphorylation on a serine residue. Appears to be co-transcribed with Rv0932c|pstS2. P9WI79 No regulatory proteins 1RWI,1RWL O05871 P9WI79 GO:0006468,GO:0004674,GO:0005524 2.7.11.1 P9WI79 ML0743 MMAR_4577 Mb0954c,Mb0955c NC_000962.3 Mycobrowser_v4 CDS 1041264 1042094 . + 0 Rv0933 pstB Involved in active transport of inorganic phosphate across the membrane (import); responsible for energy coupling to the transport system. This is one of the proteins required for binding-protein-mediated phosphate transport. Have ATP-binding ability and ATPase activity. Phosphate-transport ATP-binding protein ABC transporter PstB Rv0933, (MTCY08D9.06c), len: 276 aa. PstB, phosphate-transport ATP-binding protein ABC transporter (see citations below), thermostable ATPase, highly similar to others e.g. NP_348334.1|NC_003030 ATPase component of ABC-type phosphate transport system from Clostridium acetobutylicum (249 aa); NP_212352.1|NC_001318 phosphate ABC transporter ATP-binding protein (pstB) from Borrelia burgdorferi (260 aa); NP_390375.1|NC_000964 phosphate ABC transporter (ATP-binding protein) from Bacillus subtilis (269 aa), FASTA scores: opt: 762, E(): 0, (47.7% identity in 243 aa overlap); etc. Also similar to other M. tuberculosis ABC transporters e.g. MTCY253.24, E(): 2.5e-15 and MTCY359.14c, E(): 3.4e-15. Contains PS00211 ABC transporters family signature, and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters). Magnesium or calcium seem to have no influence on the functionality of this enzyme. P9WQK9 No cell wall and cell processes P95302 P9WQK9 GO:0005315,GO:0015415,GO:0006817,GO:0005886,GO:0005524 3.6.3.27 P9WQK9 ML0741,ML0741c Mb0957,Mb0958 NC_000962.3 Mycobrowser_v4 CDS 1043299 1044315 . + 0 Rv0935 pstC1 Involved in active transport of inorganic phosphate across the membrane (import); responsible for the translocation of the substrate across the membrane. This is one of the proteins required for binding-protein-mediated phosphate transport. Phosphate-transport integral membrane ABC transporter PstC1 Rv0935, (MTCY08D9.04c), len: 338 aa. PstC1, phosphate-transport integral membrane ABC transporter (see citations below), highly similar to others e.g. NP_104768.1|NC_002678|pstC phosphate ABC transporter permease protein from Mesorhizobium loti (327 aa); NP_245372.1|NC_002663|PstC PstC protein from Pasteurella multocida (320 aa); P45191|PSTC_HAEIN phosphate transport system permease from Haemophilus influenza (315 aa), FASTA scores: opt: 667, E(): 0, (36.2% identity in 309 aa overlap); etc. Also similar to Rv0929|MTCY21C12.23|PSTC2 probable transmembrane ABC transporter component of phosphate uptake system from Mycobacterium tuberculosis (324 aa), FASTA scores: opt: 487, E(): 4.1e-21, (32.3% identity in 303 aa overlap); and shows slight similarity to MTCY08D9.03c|PSTA2|Rv0936 probable transmembrane ABC transporter component of phosphate uptake system from Mycobacterium tuberculosis (301 aa). Contains binding-protein-dependent transport systems inner membrane comp signature (PS00402). P9WG07 No cell wall and cell processes P0A628 P9WG07 GO:0016021,GO:0006817,GO:0005886,GO:0005315 P9WG07 ML0739,ML0739c Mb0960 NC_000962.3 Mycobrowser_v4 CDS 1044317 1045222 . + 0 Rv0936 pstA2 Involved in active transport of inorganic phosphate across the membrane (import); responsible for the translocation of the substrate across the membrane. This is one of the proteins required for binding-protein-mediated phosphate transport. Phosphate-transport integral membrane ABC transporter PstA2 Rv0936, (MTCY08D9.03c), len: 301 aa. PstA2, phosphate-transport integral membrane ABC transporter (see citations below), highly similar to others e.g. NP_442269.1|NC_000911|PstA phosphate transport system permease protein from Synechocystis sp. strain PCC 6803 (287 aa); NP_232473.1|NC_002506 phosphate ABC transporter permease protein from Vibrio cholerae (289 aa); P07654|PSTA_ECOLI phosphate transport system permease from Escherichia coli (296 aa), FASTA scores: opt: 464, E(): 6.7e-24, (30.5% identity in 282 aa overlap); etc. Also similar to O86345|MTCY21C12.24|PSTA1|Rv0930 probable transmembrane ABC transporter component of phosphate uptake system from Mycobacterium tuberculosis (304 aa), FASTA scores: opt: 369, E(): 6.1e-15, (32.7% identity in 248 aa overlap). Contains binding-protein-dependent transport systems inner membrane comp signature (PS00402). P9WG09 No cell wall and cell processes P0A626 P9WG09 GO:0016021,GO:0006817,GO:0005886,GO:0005315 P9WG09 ML0738,ML0738c Mb0961 NC_000962.3 Mycobrowser_v4 CDS 1045199 1046020 . - 0 Rv0937c mku Involved in DNA double-strand break repair, by nonhomologous end joining (NHEJ). Interacts with LIGD (Rv0938). DNA end-binding protein, Mku Rv0937c, (MTCY08D9.02), len: 273 aa. Mku, DNA end-binding protein, highly similar to others e.g. SC6G9.24c|T35620|AL079356 hypothetical protein from Streptomyces coelicolor (365 aa), FASTA scores: opt: 648, E(): 0, (36.5% identity in 274 aa overlap); Z99110|BSUB0007_223|NP_389224.1|NC_000964 hypothetical proteins from Bacillus subtilis (311 aa), FASTA scores: opt: 623, E(): 1.1e-31, (33.9% identity in 274 aa overlap); O28548|AE000984|AF1726|NP_070554.1|NC_000917 conserved hypothetical protein from Archaeoglobus fulgidus (286 aa), FASTA scores: opt: 583, E(): 0, (36.6% identity in 262 aa overlap); etc. P9WKD9 No information pathways O05866 P9WKD9 GO:0003677,GO:0006303,GO:0004003 P9WKD9 ML2092 MMAR_4575 MSMEG_5580 Mb0962c NC_000962.3 Mycobrowser_v4 CDS 1050593 1051459 . - 0 Rv0940c Rv0940c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0940c, (MTCY10D7.34), len: 288 aa. Possible oxidoreductase, similar to hypothetical proteins and oxidoreductases e.g. AAK38097.1|AF323606_3|AF323606 putative F420-dependent dehydrogenase from Rhodococcus erythropolis (295 aa); AAG52987.1|AF040570|Rif17 putative alkanal monooxygenase from Amycolatopsis mediterranei (356 aa); etc. Also similar to putative oxidoreductases from Mycobacterium tuberculosis such as Rv0953c|P71557|YT21_MYCTU (282 aa), FASTA scores: opt: 311, E(): 3.7e-08, (31.0% identity in 248 aa overlap), Rv3079c (275 aa), Rv0791c (347 aa), etc. P9WKP1 No intermediary metabolism and respiration P64761 P9WKP1 P9WKP1 MMAR_4568 Mb0965c NC_000962.3 Mycobrowser_v4 CDS 2205277 2205549 . - 0 Rv1962A vapB35 Unknown Possible antitoxin VapB35 Rv1962A, len: 90 aa. Possible vapB35, antitoxin, part of toxin-antitoxin (TA) operon with Rv1962c, see Arcus et al. 2005. Similar to others in M. tuberculosis e.g. Rv3385c, Rv3407, Rv0626 P9WF17 No virulence, detoxification, adaptation P9WF17 P9WF17 NC_000962.3 Mycobrowser_v4 CDS 1077975 1078334 . + 0 Rv0967 csoR Involved in transcriptional mechanism; repression of the CSO operon. Copper-sensitive operon repressor CsoR Rv0967, (MTCY10D7.07c), len: 119 aa. CsoR, copper-sensitive operon repressor, part of cso operon (See Liu et al., 2007), similar to hypothetical proteins from several organisms e.g. AE002074|AE002074_11 from Deinococcus radiodurans (102 aa), FASTA scores: opt: 233, E(): 8.6e-10, (47.0% identity in 83 aa overlap); O32222|Z99121|YVGZ from Bacillus subtilis (101 aa), FASTA scores: opt:228, E(): 3.2e-15, (38.0% identity in 92 aa overlap); etc. Also similar to Mycobacterium tuberculosis hypothetical proteins Rv0190, and Rv1766. P9WP49 No regulatory proteins 2HH7 P71543 P9WP49 GO:0005737,GO:0046872,GO:0045449,GO:0006350,GO:0003677 P9WP49 MMAR_4874 Mb0992 NC_000962.3 Mycobrowser_v4 CDS 1048412 1050346 . + 0 Rv0939 Rv0939 Function unknown; probably involved in cellular metabolism, possibly in a degradation pathway. Possible bifunctional enzyme: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (HHDD isomerase) + cyclase/dehydrase Rv0939, (MTCY10D7.35c), len: 644 aa. Possible bifunctional enzyme, including 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase activity, and cyclase/dehydrase activity. N-terminal part similar to many isomerases e.g. NP_343861.1|NC_002754 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (hpcE-1) from Sulfolobus solfataricus (318 aa); NP_068932.1|NC_000917 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (hpcE-1) from Archaeoglobus fulgidus (324 aa), FASTA scores: opt: 400, E(): 5.8e-15, (33.9% identity in 289 aa overlap); etc. And C-terminal part highly similar to many cyclases/dehydrases e.g. AAK61721.1|AY033994 cyclase-like protein from Streptomyces aureofaciens (305 aa); CAC44204.1|AL593842 cyclase from Streptomyces coelicolor (297 aa), FASTA scores: opt: 375, E(): 2.7e-26, (35.6% identity in 284 aa overlap); NP_343860.1|NC_002754 putative Cyclase/dehydrase from Sulfolobus solfataricus (308 aa); etc. Also similar to Rv2993c hypothetical protein from Mycobacterium tuberculosis. O86346 No intermediary metabolism and respiration O86346 O86346 GO:0016853,GO:0008152,GO:0016787 5.3.3.- O86346 ML2089,ML2089c MMAR_4572 Mb0964 NC_000962.3 Mycobrowser_v4 CDS 1052696 1053736 . - 0 Rv0943c Rv0943c Function unknown; probably involved in cellular metabolism. Probable monooxygenase Rv0943c, (MTCY10D7.31), len: 346 aa. Possible monooxygenase, similar in part to others e.g. NP_250229.1|NC_002516 probable flavin-containing monooxygenase from Pseudomonas aeruginosa (527 aa); AAC36351.1|AF090329 cyclohexanone monooxygenase homolog from Pseudomonas fluorescens (437 aa); CAB59668.1|AL132674 monooxygenase from Streptomyces coelicolor (519 aa); etc. Also similar to putative monooxygenases from Mycobacterium tuberculosis e.g. Rv1393c|P71662|CY21B4.10C (492 aa). FASTA scores: opt: 129, E(): 8.5e-21, (27.5% identity in 236 aa overlap); Rv0892 (495 aa); Rv3049c (524 aa); etc. P9WKN7 No intermediary metabolism and respiration P9WKN7 P9WKN7 MMAR_4565 Mb0968c NC_000962.3 Mycobrowser_v4 CDS 1112384 1113460 . + 0 Rv0996 Rv0996 Unknown Probable conserved transmembrane protein Rv0996, (MTCI237.10), len: 358 aa. Probable conserved transmembrane protein, equivalent to AL035500|MLCL373_25 putative membrane protein from Mycobacterium leprae (342 aa), FASTA scores: (66.4% identity in 360 aa overlap). Contains possible signal sequence and other hydrophobic domains. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O05579 No cell wall and cell processes O05579 GO:0016021 O05579 ML0185 MSMEG_5468 Mb1023 NC_000962.3 Mycobrowser_v4 CDS 1057300 1057530 . - 0 Rv0947c Rv0947c Function unknown; thought to be involved in mycolic acid biosynthesis. Probable mycolyl transferase, pseudogene Rv0947c, (MTCY10D7.27), len: 76 aa. Probable mycolyl transferase pseudogene, similar to part of P31953|A85C_MYCTU|fbpC2 antigen 85-c precursor (85c) (fibronectin-binding protein C) from Mycobacterium tuberculosis (340 aa), FASTA scores: opt: 213, E(): 2e-08, (69.6% identity in 46 aa overlap). Yes lipid metabolism Mb0972c NC_000962.3 Mycobrowser_v4 CDS 1057646 1057963 . - 0 Rv0948c Rv0948c Involved in the shikimate pathway. Converts chorismate to prephenate in the biosynthesis of tyrosine and phenylalanine. Chorismate mutase Rv0948c, (MTCY10D7.26), len: 105 aa. Chorismate mutase, AroQ class (See Prakash et al., 2005; Schneider et al., 2008), equivalent to NP_301237.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (105 aa). Also similar (except in N-terminus) to SCD63.16c|CAB82023.1|AL161755 hypothetical protein from Streptomyces coelicolor (110 aa); and to N-terminus of two chorismate mutase/prephenate dehydratase. P9WIC1 No intermediary metabolism and respiration 2QBV,2VKL,2W19,2W1A P64767 P9WIC1 GO:0046417 P9WIC1 ML0151,ML0151c MMAR_4555 MSMEG_5536 Mb0973c NC_000962.3 Mycobrowser_v4 CDS 1060656 1061654 . - 0 Rv0950c Rv0950c Function unknown Conserved hypothetical protein Rv0950c, (MTCY10D7.24), len: 332 aa. Conserved hypothetical protein, highly similar to AL035500|MLCL373.02c|T45433 hypothetical protein from Mycobacterium leprae (343 aa), FASTA scores: opt: 1500, E(): 0, (71.0% identity in 331 aa overlap). C-terminus highly similar to part of various proteins e.g. C-terminal part of NP_441943.1|NC_000911|NlpD lipoprotein from Synechocystis sp (715 aa); N-terminal part of NP_066789.1|NC_002576 putative peptidase from Rhodococcus equi (546 aa); C-terminal part of NP_212396.1|NC_001318 conserved hypothetical protein from Borrelia burgdorferi (417 aa); C-terminal part of P33648|NLPD_ECOLI|nlpd lipoprotein from Escherichia coli (379 aa), FASTA scores: opt: 276, E(): 2e-10, (29.9% identity in 234 aa overlap); etc. P71560 No conserved hypotheticals P71560 P71560 GO:0006508,GO:0004222 P71560 ML0154,ML0154c MMAR_4551 MSMEG_5526 Mb0975c NC_000962.3 Mycobrowser_v4 CDS 1075297 1076097 . - 0 Rv0963c Rv0963c Function unknown Conserved hypothetical protein Rv0963c, (MTCCY10D7.11), len: 266 aa. Conserved hypothetical protein, similar in part to other conserved hypothetical proteins from Mycobacterium tuberculosis e.g. Rv2797c|MTCY16B7.46 (562 aa), FASTA scores: E(): 1.2e-23, (39.0% identity in 254 aa overlap); Rv2542 (403 aa); Rv2079 (656 aa). Also similar in part to AL133423|SC4A7_3 hypothetical secreted protein from Streptomyces coelicolor (406 aa), FASTA scores: opt: 231, E(): 6.8e-07, (31.4% identity in 204 aa overlap); and SCH10.21c|T36533 hypothetical protein from Streptomyces coelicolor (329 aa). P9WKM7 No conserved hypotheticals P64777 P9WKM7 P9WKM7 ML0171 Mb0988c NC_000962.3 Mycobrowser_v4 CDS 1977328 1977885 . - 0 Rv1749c Rv1749c Unknown Possible integral membrane protein Rv1749c, (MTCY28.12c-MTCY04C12.33c), len: 185 aa. Possible integral membrane protein, similar to O27914|AE000940 hypothetical protein MTH1892 from Methanobacterium thermoautotrophicum (168 aa), fasta scores: E(): 9.3e-16, (37.4% identity in 123 aa overlap). O65935 No cell wall and cell processes O65935 O65935 MMAR_2101 Mb1778c NC_000962.3 Mycobrowser_v4 CDS 3136330 3136599 . - 0 Rv2828A Rv2828A Unknown Conserved hypothetical protein Rv2828A, len: 89 aa. Conserved hypothetical protein, present in many mycobacteria. Equivalent to BCG2848c and Mb2852A (100% identity to both in 89 aa overlap) I6YAC9 No conserved hypotheticals I6YAC9 NC_000962.3 Mycobrowser_v4 CDS 1063140 1064051 . + 0 Rv0952 sucD Involved in tricarboxylic acid cycle [catalytic activity: ATP + succinate + CoA = ADP + succinyl-CoA + phosphate]. Probable succinyl-CoA synthetase (alpha chain) SucD (SCS-alpha) Rv0952, (MTCY10D7.22c), len: 303 aa. Probable sucD, succinyl-CoA synthetase, alpha chain, equivalent to AL035500|MLCL373_4|NP_301242.1|NC_002677 succinyl-CoA synthase [alpha] chain from Mycobacterium leprae (300 aa), FASTA score: (86.3% identity in 300 aa overlap). Also highly similar to others e.g. CAB92672.1|AL356832 from Streptomyces coelicolor (294 aa); P53591|SUCD_COXBU from Escherichia coli (288 aa), FASTA scores: opt: 855, E(): 0, (53.8% identity in 286 aa overlap); etc. Contains PS00399 ATP-citrate lyase and succinyl-CoA ligases active site, and PS00017 ATP/GTP-binding site motif A (P-loop). P9WGC7 No intermediary metabolism and respiration P71558 P9WGC7 GO:0003878,GO:0004775,GO:0006099,GO:0005524 6.2.1.5 P9WGC7 ML0156 MMAR_4549 MSMEG_5524 Mb0977 NC_000962.3 Mycobrowser_v4 CDS 1065127 1066038 . + 0 Rv0954 Rv0954 Unknown Probable conserved transmembrane protein Rv0954, (MTCY10D7.20c), len: 303 aa. Probable conserved transmembrane protein, highly similar to 34KD_MYCPA|Q04959 34 kDa antigenic protein from Mycobacterium paratuberculosis (298 aa), FASTA scores: opt: 1023, E(): 7.2e-36, (59.3% identity in 305 aa overlap); AAC69251.1|U82111 34 kDa antigen precursor from Mycobacterium leprae (336 aa); and AL035500|MLCL373.06 hypothetical membrane protein from Mycobacterium leprae (297 aa), FASTA score: (55.6% identity in 315 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WIR9 No cell wall and cell processes P9WIR9 GO:0005886,GO:0016021 ML0158 MMAR_4545 MSMEG_5518 Mb0979 NC_000962.3 Mycobrowser_v4 CDS 1066078 1067445 . + 0 Rv0955 Rv0955 Unknown Probable conserved integral membrane protein Rv0955, (MTCY10D7.19c), len: 455 aa. Probable conserved integral membrane protein, highly similar to AL035500|MLCL373_6 putative membrane protein from Mycobacterium leprae (430 aa), FASTA score: (75.9% identity in 419 aa overlap); and AAL05878.1|AF411607_2|AF411607 unknown protein from Mycobacterium avium subsp. paratuberculosis (409 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WKN3 No cell wall and cell processes P9WKN3 GO:0005886,GO:0016021 ML0159 MMAR_4544 MSMEG_5517 Mb0980 NC_000962.3 Mycobrowser_v4 CDS 1088493 1090175 . - 0 Rv0976c Rv0976c Function unknown Conserved hypothetical protein Rv0976c, (MTV044.04c), len: 560 aa. Conserved hypothetical protein, highly similar to others e.g. CAB95890.1|AL359988 conserved hypothetical protein from Streptomyces coelicolor (558 aa); P_251576.1|NC_002516 hypothetical protein from Pseudomonas aeruginosa (600 aa); etc. N-terminal part highly similar to AL035500|MLCL373_14 probable pseudogene from Mycobacterium leprae (163 aa), FASTA score: (50.0% identity in 122 aa overlap). O86320 No conserved hypotheticals O86320 O86320 O86320 MMAR_4531 MSMEG_5490 Mb1001c NC_000962.3 Mycobrowser_v4 CDS 1069883 1071262 . + 0 Rv0958 Rv0958 Chelation, introducing a magnesium ion into specific substrate. Possible magnesium chelatase Rv0958, (MTCY10D7.16c), len: 459 aa. Possible magnesium chelatase, similar to others (especially in N-terminal parts) e.g. NP_296313.1|NC_001263|AE002088_10 putative magnesium protoporphyrin chelatase from Deinococcus radiodurans (487 aa), FASTA scores: opt: 1148, E(): 0, (42.4% identity in 450 aa overlap); Q44498|CHLI_ANAVA magnesium-chelatase subunit CHLI from Anabaena variabilis (338 aa); T31460 probable magnesium chelatase chain I bchI from Heliobacillus mobilis (363 aa); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). P71552 No intermediary metabolism and respiration P71552 P71552 GO:0005622,GO:0016829,GO:0006355,GO:0008134,GO:0005524 4.99.1.- P71552 MMAR_4541 MSMEG_5512 Mb0983 NC_000962.3 Mycobrowser_v4 CDS 1071255 1073273 . + 0 Rv0959 Rv0959 Function unknown Conserved hypothetical protein Rv0959, (MTCY10D7.15c), len: 672 aa. Conserved hypothetical protein, similar to AE002069|AE002069_12 hypothetical protein from Deinococcus radiodurans (403 aa), FASTA scores: opt: 395, E(): 1.3e-15, (26.8% identity in 426 aa overlap). Contains a single copy at the N-terminus of a short repeat found three times in the M. tuberculosis ORF O33341|MTV003.05c|AL008883. P9WKN1 No conserved hypotheticals P9WKN1 ML0163 MMAR_4540 MSMEG_5511 Mb0984 NC_000962.3 Mycobrowser_v4 CDS 1073327 1073548 . + 0 Rv0959A vapB9 Unknown Possible antitoxin VapB9 Rv0959A, len: 73 aa. Possible vapB9, antitoxin, part of toxin-antitoxin (TA) operon with Rv0960 (See Arcus et al., 2005; Pandey and Gerdes, 2005). Weakly similar to others in Mycobacterium tuberculosis e.g. Rv1721c P9WJ55 No virulence, detoxification, adaptation P9WJ55 NC_000962.3 Mycobrowser_v4 CDS 1073545 1073928 . + 0 Rv0960 vapC9 Unknown Possible toxin VapC9 Rv0960, (MTCY10D7.14c), len: 127 aa. Possible vapC9, toxin, part of toxin-antitoxin (TA) operon with Rv0959A, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. Rv0065|MTV030.08 (133 aa), FASTA scores: E(): 1.5e-14, (38.3% identity in 128 aa overlap), Rv1720c (129 aa), and Rv0549c (137 aa). P9WFA9 No virulence, detoxification, adaptation P64773 P9WFA9 P9WFA9 Mb0985 NC_000962.3 Mycobrowser_v4 CDS 1074074 1074421 . + 0 Rv0961 Rv0961 Unknown Probable integral membrane protein Rv0961, (MTCY10D7.13c), len: 115 aa. Probable integral membrane protein. P9WKM9 No cell wall and cell processes P9WKM9 GO:0005886,GO:0016021 P9WKM9 Mb0986 NC_000962.3 Mycobrowser_v4 CDS 1074440 1075114 . - 0 Rv0962c lprP Unknown Possible lipoprotein LprP Rv0962c, (MTCY10D7.12), len: 224 aa. Possible lprP, lipoprotein. Contains possible N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. P9WK39 No cell wall and cell processes P9WK39 GO:0005886 P9WK39 ML0172 Mb0987c NC_000962.3 Mycobrowser_v4 CDS 1076778 1077197 . - 0 Rv0965c Rv0965c Function unknown Conserved hypothetical protein Rv0965c, (MTCY10D7.09), len: 139 aa. Conserved hypothetical protein, showing weak similarity with Rv2798c|MTCY16B7.45 conserved hypothetical protein from Mycobacterium tuberculosis (108 aa), FASTA scores: E(): 5.6e-12, (38.9% identity in 90 aa overlap). Equivalent to AAK45242.1 from Mycobacterium tuberculosis strain CDC1551 (146 aa) but shorter 7 aa. P9WKM3 No conserved hypotheticals P9WKM3 ML0170 Mb0990c NC_000962.3 Mycobrowser_v4 CDS 1081052 1081684 . + 0 Rv0970 Rv0970 Unknown Probable conserved integral membrane protein Rv0970, (MTCY10D7.04c), len: 210 aa. Probable conserved integral membrane protein, part of cso operon, equivalent to NP_302348.1|NC_002677 probable integral membrane protein from Mycobacterium leprae (210 aa). P9WKL7 No cell wall and cell processes P9WKL7 GO:0005886,GO:0016021 ML1997,ML1997c MMAR_1432,MMAR_4877 Mb0995 NC_000962.3 Mycobrowser_v4 CDS 1081775 1082584 . - 0 Rv0971c echA7 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Probable enoyl-CoA hydratase EchA7 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv0971c, (MTCY10D7.03), len: 269 aa. Probable echA7, enoyl-CoA hydratase, similar to many e.g. CAB95895.1|AL359988 putative enoyl CoA hydratase from Streptomyces coelicolor (247 aa); P24162|ECHH_RHOCA enoyl-CoA hydratase from Rhodobacter capsulatus (257 aa), FASTA scores: opt: 369, E(): 2.6e-15, (33.7% identity in 246 aa overlap); etc. P71540 No lipid metabolism P71540 P71540 GO:0016853,GO:0008152,GO:0004300 4.2.1.17 P71540 ML0168 MMAR_4536 MSMEG_5495 Mb0996c NC_000962.3 Mycobrowser_v4 CDS 1082584 1083750 . - 0 Rv0972c fadE12 Function unknown, but involved in lipid degradation. Acyl-CoA dehydrogenase FadE12 Rv0972c, (MTCY10D7.02), len: 388 aa. fadE12, acyl-CoA dehydrogenase, highly similar to many e.g. CAB95893.1|AL359988 putative acyl CoA dehydrogenase from Streptomyces coelicolor (382 aa); P45857|ACDB_BACSU from Bacillus subtilis (379 aa), FASTA scores: opt: 576, E(): 2.3e-26, (29.7% identity in 381 aa overlap); etc. P9WQG3 No lipid metabolism P71539 P9WQG3 GO:0003995,GO:0055114,GO:0050660 1.3.99.- P9WQG3 ML0167 MMAR_4535 MSMEG_5494 Mb0997c NC_000962.3 Mycobrowser_v4 CDS 1085756 1087345 . - 0 Rv0974c accD2 Involved in fatty acid metabolism. Probable acetyl-/propionyl-CoA carboxylase (beta subunit) AccD2 Rv0974c, (MTV044.02c), len: 529 aa. Probable accD2, acetyl-/propionyl-CoA carboxylase (beta subunit), highly similar to many e.g. CAB95891.1|AL35998 putative acetyl/propionyl CoA carboxylase beta subunit from Streptomyces coelicolor (532 aa); NP_250704.1|NC_002516 probable acyl-CoA carboxyltransferase beta chain from Pseudomonas aeruginosa (535 aa); BAB16296.1|AB039884 acetyl-CoA carboxylase carboxyltransferase from Myxococcus xanthus (538 aa); NP_420973.1|NC_002696 putative propionyl-CoA carboxylase beta subunit from Caulobacter crescentus (530 aa); etc. Also similar to other from Mycobacterium tuberculosis: Rv2502c|ACCD1, Rv3799c|ACCD4, etc. Could belong to the ACCD/PCCB family. O86318 No lipid metabolism O86318 O86318 GO:0016874 6.4.1.- O86318 ML0165 MMAR_4533 MSMEG_5492 Mb0999c NC_000962.3 Mycobrowser_v4 CDS 1994671 1995054 . - 0 Rv1761c Rv1761c Unknown Possible exported protein Rv1761c, (MTCY28.27c), len: 127 aa. Possibly exported protein with hydrophobic stretch or TMhelix at aa 15-37. O06796 No cell wall and cell processes 2K3M O06796 O06796 MMAR_2636 Mb1792c NC_000962.3 Mycobrowser_v4 CDS 1094886 1095059 . + 0 Rv0979A rpmF Involved in translation mechanism. 50S ribosomal protein L32 RpmF Rv0979A, len: 57 aa. rpmF, 50S ribosomal protein L32, similar to others e.g. rpmF|Q9RL50 probable 50S ribosomal protein from Streptomyces coelicolor (56 aa), FASTA scores: E(): 5.1e-09, (63.45% identity in 52 aa overlap); etc. Belongs to the L32P family of ribosomal proteins. P0A5V8 No information pathways P0A5V8 P0A5V8 GO:0003735,GO:0006412,GO:0015934 ML0173 MMAR_4530 MSMEG_5489 Mb1005 NC_000962.3 Mycobrowser_v4 CDS 1093361 1094356 . - 0 Rv0978c PE_PGRS17 Function unknown PE-PGRS family protein PE_PGRS17 Rv0978c, (MTV044.06c), len: 331 aa. PE_PGRS17, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to others e.g. Z95387|MTCY1A10_19 from Mycobacterium tuberculosis (461 aa), FASTA score: (73.6% identity in 277 aa overlap); etc. Q79FU2 No PE/PPE Q79FU2 Q79FU2 Q79FU2 Mb1003c NC_000962.3 Mycobrowser_v4 CDS 1094670 1094864 . - 0 Rv0979c Rv0979c Unknown Hypothetical protein Rv0979c, (MTV044.07c), len: 64 aa (unlikely ORF). Hypothetical unknown protein. Start codon changed since first submission (-44 aa). O53892 No conserved hypotheticals O53892 Mb1004c NC_000962.3 Mycobrowser_v4 CDS 1095078 1096451 . - 0 Rv0980c PE_PGRS18 Function unknown PE-PGRS family protein PE_PGRS18 Rv0980c, (MTV044.08c), len: 457 aa. PE_PGRS18, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002), highly similar to others e.g. Z95387|MTCY1A10_19 from Mycobacterium tuberculosis (461 aa), FASTA score: (66.7% identity in 405 aa overlap); Z95844|MTCY493_2 from Mycobacterium tuberculosis (741 aa), FASTA score: (53.0% identity in 394 aa overlap); etc. Predicted to be an outer membrane protein (See Song et al., 2008). Q79FU0 No PE/PPE Q79FU0 Q79FU0 Q79FU0 Mb1006c NC_000962.3 Mycobrowser_v4 CDS 1108578 1109171 . - 0 Rv0992c Rv0992c Function unknown Conserved hypothetical protein Rv0992c, (MTCI237.06c), len: 197 aa. Conserved hypothetical protein, equivalent to NP_301256.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (197 aa). Also similar, except in N-terminus, to other hypothetical proteins and ligases e.g. SCE87.34|CAB59679.1|AL132674 hypothetical protein from Streptomyces coelicolor (204 aa); NP_461977.1|NC_003197 putative ligase from Salmonella typhimurium (182 aa); P09160|YGFA_ECOLI hypothetical 21.1 kDa protein from Escherichia coli (182 aa), FASTA scores: opt: 191, E(): 1.1e-09, (29.5% identity in 146 aa overlap); etc. O05575 No conserved hypotheticals O05575 O05575 GO:0005524,GO:0009396,GO:0030272 O05575 ML0181,ML0181c MMAR_4522 MSMEG_5472 Mb1019c NC_000962.3 Mycobrowser_v4 CDS 1114293 1114724 . + 0 Rv0997 Rv0997 Unknown Hypothetical protein Rv0997, (MTCI237.11), len: 143 aa. Hypothetical unknown protein, equivalent to AAK45276.1 from Mycobacterium tuberculosis strain CDC1551 (87 aa) but longer 56 aa. O05580 No conserved hypotheticals O05580 ML0186 Mb1024 NC_000962.3 Mycobrowser_v4 CDS 1099066 1100460 . + 0 Rv0983 pepD Function unknown; possibly hydrolyzes peptides and/or proteins (seems to cleave preferentially after serine residues). Probable serine protease PepD (serine proteinase) (MTB32B) Rv0983, (MTV044.11), len: 464 aa. Probable pepD (alternate gene name: mtb32b), secreted or membrane serine protease (see citation below), equivalent (but longer 18 aa in N-terminus) to AL035500|MLCL373_17|T45448 probable serine proteinase from Mycobacterium leprae (452 aa), FASTA score: (74.2% identity in 466 aa overlap); and highly similar to others from Mycobacterium leprae. Also highly similar (except in N-terminus) to other proteases e.g. CAC01350.1|AL390975 putative protease from Streptomyces coelicolor (542 aa); NP_440705.1|NC_000911|HtrA serine protease from Synechocystis sp. (452 aa); NP_346646.1|NC_003028 serine protease from Streptococcus pneumoniae (393 aa); etc. Also similar in part to members of the htrA-antigen family e.g. U87242|MTU87242_3|HtrA serine protease from M. tuberculosis (542 aa), FASTA scores: opt: 846, E(): 2e-28, (40.6% identity in 392 aa overlap); and similar to other hypothetical serine proteases e.g. Rv0983, Rv0125, etc. Belongs to the serine protease family. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). O53896 No intermediary metabolism and respiration 1Y8T,2Z9I O53896 O53896 GO:0006508,GO:0004252,GO:0005515 3.4.21.- O53896 ML0176 MMAR_4527 MSMEG_5486 Mb1009 NC_000962.3 Mycobrowser_v4 CDS 1100460 1101005 . + 0 Rv0984 moaB2 Thought to be involved in molybdopterin biosynthesis. Catalyzes the dehydratation of 4A-hydroxytetrahydropterins [catalytic activity: (6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4A-hydroxypterin = (6R)-6-(L-erythro-1,2- dihydroxypropyl)-7,8-dihydro-6H-pterin + H(2)O.]. Possible pterin-4-alpha-carbinolamine dehydratase MoaB2 (PHS) (4-alpha- hydroxy-tetrahydropterin dehydratase) (pterin-4-a-carbinolamine dehydratase) (phenylalanine hydroxylase-stimulating protein) (PHS) (pterin carbinolamine dehydratase) (PCD) Rv0984, (MTV044.12), len: 181 aa. Possible moaB2, pterin-4-alpha-carbinolamine dehydratase, highly similar to NP_301253.1|NC_002677 putative molybdenum cofactor biosynthesis protein from Mycobacterium leprae (181 aa), FASTA score: (92.3% identity in 181 aa overlap). Also similar to others e.g. CAB59675.1|AL132674 molybdenum cofactor biosynthesis protein from Streptomyces coelicolor (179 aa); Q56208|MOCB_SYNP7 molybdenum cofactor biosynthesis protein CB from Synechococcus sp. (319 aa), FASTA score: (37.3% identity in 142 aa overlap); C-terminus of NP_197599.1|NC_003076 molybdopterin biosynthesis CNX1 protein from Arabidopsis thaliana (670 aa); etc. Also similar to Rv0865|MOG from Mycobacterium tuberculosis (160 aa); and other mog proteins e.g. CAC39235.1|AJ312124 Mog protein from Eubacterium acidaminophilum (162 aa). Could belong to the pterin-4-alpha-carbinolamine dehydratase family. Alternative start codon has been suggested in position 1100508. O53897 No intermediary metabolism and respiration O53897 O53897 GO:0016829,GO:0006777 O53897 ML0177 MSMEG_5485 Mb1010 NC_000962.3 Mycobrowser_v4 CDS 1122222 1123598 . - 0 Rv1005c pabB Catalyzes the biosynthesis of 4-amino-4-deoxychorismate (ADC) from chorismate and glutamine Probable para-aminobenzoate synthase component I PABD Rv1005c, (MTCI237.22c), len: 458 aa (Start-site not certain). Probable PabD, para-aminobenzoate synthase component I. Similar to PABB_ECOLI|P05041 para-aminobenzoate synthase component I from Escherichia coli (453 aa), FASTA scores: opt: 589, E(): 1.8e-27, (40.7% identity in 268 aa overlap). Similar to M. tuberculosis Rv1609, Rv3215, Rv2386c. O05591 No intermediary metabolism and respiration O05591 O05591 GO:0016829,GO:0009396 4.1.3.- O05591 ML0236,ML0236c MMAR_4488 MSMEG_5446 Mb1032c NC_000962.3 Mycobrowser_v4 CDS 1162549 1163376 . - 0 Rv1040c PE8 Function unknown PE family protein PE8 Rv1040c, (MTCY10G2.09), len: 275 aa. PE8, Member of the Mycobacterium tuberculosis PE family (see citation below), most similar to AL008967|MTV002_34 Mycobacterium tuberculosis H37Rv (275 aa), FASTA scores: opt: 1111, E(): 0, (68.6% identity in 283 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L7N667 No PE/PPE Q7D8Y6 L7N667 L7N667 ML0263,ML0263c MMAR_4451 Mb1069c NC_000962.3 Mycobrowser_v4 CDS 1102542 1105109 . + 0 Rv0987 Rv0987 Thought to be involved in active transport of adhesion component across the membrane: involved in atachment and virulence. Responsible for the translocation of the substrate across the membrane. Probable adhesion component transport transmembrane protein ABC transporter Rv0987, (MTV044.15, MTCI237.01), len: 855 aa. Probable transmembrane protein ABC transporter supposedly involved in transport of adhesion component (see citation below), whose N-terminus shows similarity with hypothetical proteins, generally transmembrane proteins, e.g. CAB96016.1|AL360055 putative ABC transport system integral membrane protein from Streptomyces coelicolor (855 aa); P44252|YCFU_HAEIN|HI1555 hypothetical protein from Haemophilus influenzae (393 aa), FASTA scores: opt: 265, E(): 1.7e-09, (23.6% identity in 402 aa overlap); etc. N- and C-termini respectively show similarity to O32735 ATTF protein (420 aa), FASTA scores: E(): 1e-09, (26.7% identity in 430 aa overlap), and G2340078 ATTG protein (359 aa), FASTA scores: E(): 2.7e-08, (27.8% identity in 356 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Believed to have been acquired by horizontal gene transfer (See Rosas-Magallanes et el., 2006; Becq et al., 2007). O53900 No cell wall and cell processes O53900 O53900 GO:0005886,GO:0016021 O53900 Mb1013,Mb1014 NC_000962.3 Mycobrowser_v4 CDS 1105116 1106276 . + 0 Rv0988 Rv0988 Unknown Possible conserved exported protein Rv0988, (MTCI237.02), len: 386 aa. Possible conserved exported protein, with potential N-terminal signal sequence, similar (except in N-terminus) to O32737|L63540 ATTH protein from Agrobacterium tumefaciens (355 aa), FASTA scores: opt: 651, E(): 5.7e-33, (33.4% identity in 344 aa overlap); and NP_231265.1|NC_002505 conserved hypothetical protein from Vibrio cholerae (372 aa). Predicted to be an outer membrane protein (See Song et al., 2008). Believed to have been acquired by horizontal gene transfer (See Rosas-Magallanes et el., 2006; Becq et al., 2007). O86370 No cell wall and cell processes O86370 O86370 GO:0015995,GO:0055114,GO:0016491,GO:0016117 O86370 Mb1015 NC_000962.3 Mycobrowser_v4 CDS 1144564 1145853 . + 0 Rv1023 eno Glycolysis [catalytic activity:2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O] Probable enolase Eno Rv1023, (MTCY10G2.26c), len: 429 aa. Probable eno, enolase, highly similar to others e.g. ENO_ECOLI|P08324 enolase from Escherichia coli (431 aa), FASTA scores: opt: 1487, E(): 0, (55.5% identity in 422 aa overlap); etc. Magnesium is required for catalysis and for stabilizing the dimer. Belongs to the enolase family. P9WNL1 No intermediary metabolism and respiration P96377 P9WNL1 GO:0005576,GO:0006096,GO:0000287,GO:0004634,GO:0000015,GO:0009986 4.2.1.11 P9WNL1 ML0255 MMAR_4462 MSMEG_5415 Mb1051 NC_000962.3 Mycobrowser_v4 CDS 2258030 2258272 . + 0 Rv2009 vapB15 Unknown Antitoxin VapB15 Rv2009, (MTCY39.08c), len: 80 aa. VapB15, antitoxin, part of toxin-antitoxin (TA) operon with Rv2010 (See Arcus et al., 2005; Pandey and Gerdes, 2005). P9WLM7 No virulence, detoxification, adaptation Q10848 P9WLM7 P9WLM7 Mb2032 NC_000962.3 Mycobrowser_v4 ncRNA 1175225 1175315 . + 0 MTB000061 mpr5 Unknown Fragment of putative small regulatory RNA mpr5, fragment of putative small regulatory RNA (See DiChiara et al., 2010), ends not mapped, ~100 nt band detected by Northern blot. No stable RNAs NC_000962.3 Mycobrowser_v4 CDS 1107443 1108099 . - 0 Rv0990c Rv0990c Unknown Hypothetical protein Rv0990c, (MTCI237.04c), len: 218 aa. Hypothetical unknown protein. O05573 No conserved hypotheticals O05573 O05573 ML0179,ML0179c MMAR_4524 MSMEG_5481 Mb1017c NC_000962.3 Mycobrowser_v4 CDS 1110269 1111549 . + 0 Rv0994 moeA1 Involved in molybdenum cofactor biosynthesis: involved in the biosynthesis of a demolybdo-cofactor (molybdopterin), necessary for molybdo-enzymes. Probable molybdopterin biosynthesis protein MoeA1 Rv0994, (MTCI237.08), len: 426 aa. Probable moeA1, molybdenum cofactor biosynthesis protein, equivalent to AL035500|MLCL373_23 putative molybdopterin biosynthesis protein from Mycobacterium leprae (424 aa), FASTA score: (88.3% identity in 426 aa overlap). Also highly similar to many e.g. CAB59677.1|AL132674 molybdopterin biosynthesis protein from Streptomyces coelicolor (424 aa); NP_385769.1|NC_003047 probable molybdopterin biosynthesis protein from Sinorhizobium meliloti (406 aa); P12281|MOEA_ECOLI molybdopterin biosynthesis moea protein from Escherichia coli (411 aa), FASTA scores: opt: 519, E(): 1.3e-24, (32.3% identity in 402 aa overlap); etc. Also similar to MOEA2|Rv0438c|MTV037.02c probable molybdopterin biosynthesis protein from Mycobacterium tuberculosis (405 aa). Note that previously known as moeA. P9WJQ7 No intermediary metabolism and respiration O05577 P9WJQ7 GO:0046872,GO:0016740,GO:0006777 2.-.-.- P9WJQ7 ML0183 MMAR_4520 MSMEG_5470 Mb1021 NC_000962.3 Mycobrowser_v4 CDS 1111612 1112223 . + 0 Rv0995 rimJ Acetylates the N-terminal alanine of ribosomal protein S5 [catalytic activity: acetyl-CoA + ribosomal-protein L-alanine = CoA + ribosomal-protein N-acetyl-L-alanine]. Ribosomal-protein-alanine acetyltransferase RimJ (acetylating enzyme for N-terminal of ribosomal protein S5) Rv0995, (MTCI237.09), len: 203 aa. RimJ, ribosomal-protein-alanine acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Equivalent to AL035500|MLCL373_24 probable acyltransferase from Mycobacterium leprae (218 aa), FASTA scores: (86.0% identity in 200 aa overlap). Also similar to others and many acyltransferases e.g. BAB69252.1|AB070946 possible acyltransferase from Streptomyces avermitilis (156 aa); NP_385025.1|NC_003047 probable ribosomal-protein-alanine acetyltransferase from Sinorhizobium meliloti (203 aa); P09454|RIMJ_ECOLI|B1066|Z1703|ECS1444 ribosomal-protein-alanine acetyltransferase from Escherichia coli strains K12 and O157:H7 (194 aa), FASTA scores: opt: 247, E(): 1.5e-10, (26.9% identity in 186 aa overlap). Belongs to the acetyltransferase family, RIMJ subfamily. O05578 No information pathways O05578 GO:0008999,GO:0005840,GO:0008152 2.3.1.128 O05578 ML0184 MMAR_4519 MSMEG_5469 Mb1022 NC_000962.3 Mycobrowser_v4 CDS 1160855 1161151 . - 0 Rv1038c esxJ Function unknown ESAT-6 like protein EsxJ (ESAT-6 like protein 2) Rv1038c, (MT1067, MTCY10G2.11), len: 98 aa. EsxJ, ESAT-6 like protein (see Gey Van Pittius et al., 2001), similar to Q49945|U1756C, Mycobacterium leprae (100 aa), FASTA scores: opt: 375, E(): 7.7e-21, (58.3% identity in 96 aa overlap). Member of M. tuberculosis hypothetical QILSS protein family with Rv1197, Rv1792, Rv2347c and Rv3620c. Belongs to the ESAT6 family. P9WNJ9 No cell wall and cell processes P9WNJ9 P9WNJ9 ML0262,ML0262c MMAR_2674,MMAR_3654,MMAR_3663,MMAR_4453,MMAR_5120 Mb1067c NC_000962.3 Mycobrowser_v4 CDS 1116531 1117148 . - 0 Rv1000c Rv1000c Function unknown Conserved hypothetical protein Rv1000c, len: 205 aa. Conserved hypothetical protein, equivalent to ML0190|NP_301263.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (205 aa). Also highly similar to SC5F8.12c|CAB93740.1|AL357613 hypothetical protein from Streptomyces coelicolor (210 aa), FASTA scores: E(): 2.1e-45, (56.8% identity); 9106290|AAF84108.1|AE003963_5|NP_298588.1|NC_002488 protein described as DNA repair system specific for alkylated DNA from Xylella fastidiosa (200 aa), FASTA scores: E(): 3.4e-14, (38.55% identity); and similar in C-terminus to other hypothetical proteins. Note that replaces original Rv1000 predicted on other strand. L7N6A4 No conserved hypotheticals L7N6A4 L7N6A4 ML0190,ML0190c MMAR_4493 MSMEG_5451 Mb1027c NC_000962.3 Mycobrowser_v4 CDS 1117185 1118393 . + 0 Rv1001 arcA Arginine degradation [catalytic activity:L-arginine + H(2)O = L-citrulline + NH(3)] Probable arginine deiminase ArcA (adi) (ad) (arginine dihydrolase) Rv1001, (MTCI237.16), len: 402 aa. Probable arcA, arginine deiminase, similar to e.g. ARCA_PSEAE|P13981 arginine deiminase (417 aa), fasta scores: opt: 581, E(): 1.4e-31, (39.4% identity in 411 aa overlap); also similar to SAGP_STRPY|P16962 streptococcal acid glycoprotein (410 aa), FASTA scores, opt: 823, E():0, (38.3% identity in 402 aa overlap). Belongs to the arginine deiminase family. P9WQ05 No intermediary metabolism and respiration P63551 P9WQ05 GO:0016990,GO:0005737,GO:0006527 3.5.3.6 P9WQ05 ML0191 MMAR_4492 MSMEG_5448 Mb1028 NC_000962.3 Mycobrowser_v4 CDS 1118428 1119939 . - 0 Rv1002c Rv1002c Unknown Conserved membrane protein Rv1002c, (MTCI237.17c), len: 503 aa. Conserved membrane protein. Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). Similar to AL132674|SCE87.05 hypothetical protein from Streptomyces coelicolor (591 aa), FASTA scores: opt: 666, E(): 0, (39.0% identity in 546 aa overlap); weakly similar and to TSCC_PSEAM|P55019 thiazide-sensitive sodium-chloride cotransporter from Pseudopleuronectes americanus (1023 aa), FASTA scores: opt: 44, E(): 4.2e-06, (22.4% identity in 326 aa overlap). P9WN05 No cell wall and cell processes O05586 P9WN05 GO:0000030,GO:0005886,GO:0006493,GO:0016021 2.4.-.- ML0192,ML0192c MMAR_4491 MSMEG_5447 Mb1029c NC_000962.3 Mycobrowser_v4 CDS 1120022 1120879 . + 0 Rv1003 Rv1003 Function unknown Conserved protein Rv1003, (MTCI237.19), len: 285 aa. Conserved protein, similar to others e.g. AL132674|SCE87.04 Streptomyces coelicolor (286 aa), FASTA scores: opt: 877, E(): 0, (53.2% identity in 280 aa overlap); and YRAL_ECOLI|P45528 hypothetical 31.3 kd protein (286 aa), FASTA scores: opt: 561, E(): 4.4e-27, (36.9% identity in 279 aa overlap). P9WGW7 No conserved hypotheticals P0A640 P9WGW7 GO:0008168,GO:0008152 2.1.1.- P9WGW7 ML0193 MMAR_4490 MSMEG_5445 Mb1030 NC_000962.3 Mycobrowser_v4 CDS 1120889 1122148 . - 0 Rv1004c Rv1004c Unknown Probable membrane protein Rv1004c, (MTCI237.20c), len: 419 aa. Probable membrane protein. Contains repetitive sequences, which have similarities with elastin, and possible N-terminal signal sequence. O05589 No cell wall and cell processes O05589 MMAR_4489 Mb1031c NC_000962.3 Mycobrowser_v4 CDS 1123714 1125417 . + 0 Rv1006 Rv1006 Unknown Unknown protein Rv1006, (MTCI237.23), len: 567 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). O05592 No conserved hypotheticals O05592 ML0237 MMAR_4485 Mb1033 NC_000962.3 Mycobrowser_v4 CDS 1128091 1129179 . + 0 Rv1009 rpfB Thought to promote the resuscitation and growth of dormant, nongrowing cells. Could also stimulate the growth of several other high G+C gram+ organisms, e.g. Mycobacterium avium, Mycobacterium bovis (BCG), Mycobacterium kansasii, Mycobacterium smegmatis. Probable resuscitation-promoting factor RpfB Rv1009, (MTCI237.26), len: 362 aa. Probable rpfB, resuscitation-promoting factor (see citation below), similar to others from Mycobacterium tuberculosis: Rv2450c|MTV008.06c|RPFE probable resuscitation-promoting factor (172 aa), FASTA scores: E(): 1.9e-19, (42.9% identity in 147 aa overlap); Rv0867c|RPFA, Rv1884c|RPFC, and Rv2389c|RPFD. Possible lipoprotein; contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Interacts with RipA (see Hett et al., 2007). Predicted possible vaccine candidate (See Zvi et al., 2008). Validated new TSS at 13 codons upstream, corresponding to the alternative TTG start (see Schwenk et. al 2018). A transcriptionally regulated RNA switch/riboswitch candidate was identified in the 5' UTR of rpfB (see Schwenk et. al 2018). P9WG29 No cell wall and cell processes 1XSF,3EO5 O05594 P9WG29 GO:0016787,GO:0005576 P9WG29 ML0240 MMAR_4479 MSMEG_5439 Mb1036 NC_000962.3 Mycobrowser_v4 CDS 1127089 1127883 . + 0 Rv1008 tatD DNase involved in protein export. This sec-independent pathway is termed tat for twin-arginine translocation system. This system mainly transports proteins with bound cofactors that require folding prior to export. Probable deoxyribonuclease TatD (YJJV protein) Rv1008, (MTCI237.25), len: 264 aa. Probable tatD (alternate gene name: yjjV), deoxyribonuclease, component of twin arginine translocation protein export system (see citations below). Similar to many members of the YBL055C/YJJV family e.g. YCFH_ECOLI|P37346 Putative deoxyribonuclease ycfH (265 aa), fasta scores: opt: 487, E(): 1.4e-24, (36.7% identity in 270 aa overlap). Also similar to P37545|YABD_BACSU Putative deoxyribonuclease yabD (255 aa), FASTA scores: opt: 599, E(): 7.7e-33, (40.1% identity in 262 aa overlap). Contains PS01137 Hypothetical YBL055c/yjjV family signature 1, and PS01091 Hypothetical YBL055c/yjjV family signature 3. O08343 No cell wall and cell processes O08343 O08343 GO:0016888 3.1.21.- O08343 ML0239 MMAR_4480 MSMEG_5440 Mb1035 NC_000962.3 Mycobrowser_v4 CDS 1129152 1130105 . + 0 Rv1010 ksgA Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. Its inactivation LEADS to kasugamycin resistance Probable dimethyladenosine transferase KsgA (S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase) (16S rRNA dimethylase) (high level kasugamycin resistance protein KsgA) (kasugamycin dimethyltransferase) Rv1010, (MTCI237.27), len: 317 aa. Probable ksgA, dimethyladenosine transferase, similar to many e.g. KSGA_BACSU|P37468 dimethyladenosine transferase from Bacillus subtilus (292 aa), FASTA scores: opt: 524, E(): 1.5e-28, (37.2% identity in 274 aa overlap); similar to Mycobacterium tuberculosis hypothetical protein Rv1988. Contains PS01131 Ribosomal RNA adenine dimethylases signature. P9WH07 No information pathways P66660 P9WH07 GO:0005737,GO:0000179,GO:0000154,GO:0003723 2.1.1.- P9WH07 ML0241 MMAR_4478 MSMEG_5438 Mb1037 NC_000962.3 Mycobrowser_v4 CDS 1130191 1131111 . + 0 Rv1011 ispE Thought to be involved in deoxyxylulose-5-phosphate pathway (DXP) of isoprenoid biosynthesis (at the fourth step). Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol [catalytic activity: ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol]. Probable 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase IspE (CMK) (4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase) Rv1011, (MTCI237.28, MT1040), len: 306 aa. Probable ispE, 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase , similar to others e.g. Q9K3R6|ISPE_STRCO Streptomyces coelicolor (299 aa), FASTA scores: opt: 925, E(): 2.7e-49, (54.5% identity in 297 overlap); etc. Belongs to the ISPE family. P9WKG7 No intermediary metabolism and respiration P65178 P9WKG7 GO:0005524,GO:0016310,GO:0016114,GO:0050515 2.7.1.148 P9WKG7 ML0242 MMAR_4477 MSMEG_5436 Mb1038 NC_000962.3 Mycobrowser_v4 CDS 1131128 1131421 . + 0 Rv1012 Rv1012 Unknown Hypothetical protein Rv1012, (MTCI237.29), len: 97 aa. Hypothetical unknown protein. O05597 No conserved hypotheticals O05597 Mb1039,Mb1040 NC_000962.3 Mycobrowser_v4 CDS 1995054 1995842 . - 0 Rv1762c Rv1762c Unknown Unknown protein Rv1762c, (MTCY28.28c), len: 262 aa. Unknown protein. O06797 No conserved hypotheticals O06797 O06797 MMAR_2635 Mb1793c NC_000962.3 Mycobrowser_v4 CDS 1133333 1133908 . - 0 Rv1014c pth The natural substrate for this enzyme may be peptidyl-TRNAS which drop off the ribosome during protein synthesis [catalytic activity: N-substituted aminoacyl-tRNA + H(2)O = N-substituted amino acid + tRNA] Probable peptidyl-tRNA hydrolase Pth Rv1014c, (MTCY10G2.35), len: 191 aa. Probable pth, peptidyl-tRNA hydrolase, similar to PTH_ECOLI|P23932 peptidy l-trna hydrolase from Escherichia coli (194 aa), FASTA scores: opt: 472, E(): 2.3e-25, (39.6% identity in 187 aa overlap). Belongs to the PTH family. P9WHN7 No intermediary metabolism and respiration 2JRC,2Z2I,2Z2J,2Z2K P65865 P9WHN7 GO:0005737,GO:0006412,GO:0004045 3.1.1.29 P9WHN7 ML0244,ML0244c MMAR_4473 MSMEG_5432 Mb1042c NC_000962.3 Mycobrowser_v4 CDS 1133921 1134568 . - 0 Rv1015c rplY Binds to the 50S rRNA 50S ribosomal protein L25 RplY Rv1015c, (MTCY10G2.34), len: 215 aa. rplY, 50s ribosomal protein L25, similar to RL25_ECOLI|P02426 50s ribosomal protein L25 from Escherichia coli (94 aa), FASTA scores: opt: 182, E(): 2.5e-05, (38.4% identity in 86 aa overlap) and to CTC_BACSU|P14194 general stress protein from Bacillus subtilis (203 aa), FASTA scores: opt: 260, E(): 1.4e-09, (28.4% identity in 201 aa overlap). Belongs to the L25P family of ribosomal proteins. P9WHB5 No information pathways P66121 P9WHB5 GO:0005840,GO:0003735,GO:0006412,GO:0008097 P9WHB5 ML0245,ML0245c MMAR_4472 MSMEG_5431 Mb1043c NC_000962.3 Mycobrowser_v4 CDS 1135501 1136481 . - 0 Rv1017c prsA Catalyzes the formation of PRPP from ATP and ribose 5-phosphate. PRPP is then used in various biosynthetic pathways, as for example in the formation of purines, pyrimidines, histidine and tryptophan. [catalytic activity: ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate] Probable ribose-phosphate pyrophosphokinase PrsA (phosphoribosyl pyrophosphate synthetase) (PRPP synthetase) Rv1017c, (MTCY10G2.32), len: 326 aa. Probable prsA, ribose-phosphate pyrophosphokinase, highly similar to others e.g. KPRS_ECOLI|P08330 ribose-phosphate pyrophosphokinase from Escherichia coli (314 aa), FASTA scores: opt: 826, E(): 0, (43.8% identity in 317 aa overlap). Contains PS00103 Purine/pyrimidine phosphoribosyl transferases signature; contains PS00144 Asparaginase / glutaminase active site signature 1. Belongs to the ribose-phosphate pyrophosphokinase family. Cofactor: both inorganic phosphate and magnesium ion are required for enzyme stability and activity (by similarity). P9WKE3 No intermediary metabolism and respiration P65232 P9WKE3 GO:0005737,GO:0016301,GO:0000287,GO:0009116,GO:0009156,GO:0004749,GO:0005524 2.7.6.1 P9WKE3 ML0248,ML0248c MMAR_4468 MSMEG_5427 Mb1045c NC_000962.3 Mycobrowser_v4 CDS 1136573 1138060 . - 0 Rv1018c glmU Peptidoglycan and lipopolysaccharide biosynthesis Probable UDP-N-acetylglucosamine pyrophosphorylase GlmU Rv1018c, (MTCY10G2.31), len: 495 aa. Probable glmU, UDP-n-acetylglucosamine pyrophosphorylase, similar to GCAD_BACSU|P14192 UDP-n-acetylglucosamine pyrophosphorylase (456 aa), FASTA scores: opt: 1150, E(): 0, (40.0% identity in 453 aa overlap). Similar to various Mycobacterium tuberculosis sugar-phosphate transferases e.g. Rv0334, Rv1213, Rv3264c, etc. P9WMN3 No cell wall and cell processes 2QKX,3D8V,3D98,3DJ4,3FOQ P96382 P9WMN3 GO:0000902,GO:0007047,GO:0005737,GO:0019134,GO:0009103,GO:0000287,GO:0009252,GO:0008360,GO:0003977 2.3.1.157,2.7.7.23 P9WMN3 ML0249,ML0249c MMAR_4467 MSMEG_5426 Mb1046c NC_000962.3 Mycobrowser_v4 CDS 1138315 1138908 . + 0 Rv1019 Rv1019 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably TetR-family) Rv1019, (MTCY10G2.30c), len: 197 aa. Probable transcriptional regulator, similar to many memebers of the TetR family e.g. MTCY7D11.18c (34.4% identity in 189 aa overlap). Helix turn helix motif from aa 27-48 (+5.42 SD). P96381 No regulatory proteins P96381 P96381 GO:0006350,GO:0003700,GO:0006355 P96381 ML0250 MSMEG_5424 Mb1047 NC_000962.3 Mycobrowser_v4 CDS 1168704 1169228 . - 0 Rv1046c Rv1046c Unknown Hypothetical protein Rv1046c, (MTCY10G2.03), len: 174 aa. Hypothetical unknown protein. Start changed since first submission (-65 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L0T8G6 No unknown L0T8G6 Mb1075c NC_000962.3 Mycobrowser_v4 CDS 1341006 1341290 . + 0 Rv1198 esxL Unknown Putative ESAT-6 like protein EsxL (ESAT-6 like protein 4) Rv1198, (MT1236, MTCI364.10), len: 94 aa. EsxL, ESAT-6 like protein (see citation below). Member of the ESAT-6 family with Rv3619c, Rv1037c, etc. Almost identical to MTCY10G2.12 (94 aa) (97.9% identity in 94 aa overlap) and MTCY98.022c (94 aa) (94.7% identity in 94 aa overlap). Highly similar to Q49946|U1756D Mycobacterium leprae (95 aa), FASTA scores: opt: 403, E(): 1.1e-22, (64.1% identity in 92 aa overlap). seems to belong to the ESAT6 family. P9WNJ5 No cell wall and cell processes P9WNJ5 P9WNJ5 ML1056,ML1180c Mb1230 NC_000962.3 Mycobrowser_v4 CDS 1151920 1152012 . + 0 Rv1028A kdpF Thought to be involved in stabilization of the KDP complex. Probable membrane protein KdpF Rv1028A, len: 30 aa. Probable kdpF, membrane protein, showing similarity with P36937|KDPF_ECOLI|B0698.1 protein KDPF from Escherichia coli strain K12 (see citation below) (27% identity); and KdpF protein from Streptomyces coelicolor (51% identity). Q79FT7 No cell wall and cell processes Q79FT7 Q79FT7 Mb1057 NC_000962.3 Mycobrowser_v4 CDS 1159375 1160061 . - 0 Rv1035c Rv1035c Possibly required for the transposition of the insertion element IS1560. Probable transposase (fragment) Rv1035c, (MTCY10G2.14), len: 228 aa. Probable IS1560 transposase fragment, similar to parts of Rv3387|E1202305|MTV004.45 (225 aa) (47.8% identity in 67 aa overlap) and Rv3386|E1202304|MTV004.44 (234 aa) (55.1% identity in 127 aa overlap). P96366 No insertion seqs and phages P96366 Mb1064c NC_000962.3 Mycobrowser_v4 CDS 1165092 1165499 . - 0 Rv1042c Rv1042c Possibly required for the transposition of an insertion element. Probable is like-2 transposase Rv1042c, (MTCY10G2.07), len: 135 aa. Probable is like-2 transposase, similar to Q50761 transposase from Mycobacterium tuberculosis (308 aa), FASTA scores: opt: 823, E(): 0, (99.1% identity in 117 aa overlap). Second copy is Rv1149. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L0T897 No insertion seqs and phages L0T897 Mb1071c NC_000962.3 Mycobrowser_v4 CDS 1172881 1173786 . + 0 Rv1050 Rv1050 Function unknown; probably involved in cellular metabolism Probable oxidoreductase Rv1050, (MTV017.03), len: 301 aa. Probable oxidoreductase similar to many e.g. Rv1543|MTCY48.22C|Q10783 putative oxidoreductase CY48.22C (341 aa), FASTA scores: opt: 462, E(): 3e-22, (33.6% identity in 265 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53398 No intermediary metabolism and respiration O53398 O53398 GO:0055114,GO:0016491 1.-.-.- O53398 Mb1079 NC_000962.3 Mycobrowser_v4 CDS 1148427 1149107 . - 0 Rv1027c kdpE Member of the two-component regulatory system KDPD/KDPE involved in the regulation of the KDP operon. Probable transcriptional regulatory protein KdpE Rv1027c, (MTCY10G2.22), len: 226 aa. Probable KdpE, transcriptional regulatory protein, similar to others e.g. KDPE_ECOLI|P21866 kdp operon transcriptional regulatory protein from Escherichia coli strain K12 (225 aa), FASTA scores: opt: 691, E(): 0, (47.8% identity in 224 aa overlap); AL021530|SC2E9.13 from Streptomyces coelicolor (227 aa), FASTA scores: opt: 981, E(): 0, (66.4% identity in 226 aa overlap); etc. P9WGN1 No regulatory proteins P96373 P9WGN1 GO:0006355,GO:0006350,GO:0000156,GO:0000160,GO:0003677 P9WGN1 MMAR_0635 MSMEG_5396 Mb1055c NC_000962.3 Mycobrowser_v4 CDS 1149104 1151686 . - 0 Rv1028c kdpD Member of the two-component regulatory system KDPD/KDPE involved in the regulation of the KDP operon. KDPD may function as a membrane-associated protein kinase that phosphorylates KDPE|Rv1027c in response to environmental signals. Probable sensor protein KdpD Rv1028c, (MTCY10G2.21), len: 860 aa. Probable kdpD, sensor protein, similar to others e.g. KDPD_ECOLI|P21865 sensor protein from Escherichia coli strain K12 (894 aa), FASTA scores: opt: 1041, E(): 0, (32.3% identity in 888 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to universal stress protein family. P9WGL3 No regulatory proteins P96372 P9WGL3 GO:0016021,GO:0018106,GO:0005886,GO:0006950,GO:0007165,GO:0000155,GO:0000160,GO:0005524 2.7.13.3 P9WGL3 MMAR_0634 MSMEG_5395 Mb1056c NC_000962.3 Mycobrowser_v4 CDS 1155853 1156422 . + 0 Rv1031 kdpC One of the components of the high-affinity ATP-driven potassium transport (or KDP) system, which catalyzes the hydrolysis of ATP coupled with the exchange of hydrogen and potassium ions. The C subunit may be involved in assembly of the KDP complex. [catalytic activity: ATP + H(2)O + K(+)(out) = ADP + phosphate + K(+)(in)]. Probable potassium-transporting ATPase C chain KdpC (potassium-translocating ATPase C chain) (ATP phosphohydrolase [potassium-transporting] C chain) (potassium binding and translocating subunit C) Rv1031, (MTCY10G2.18c), len: 189 aa. Probable kdpC, potassium-transporting ATPase C chain (membrane protein) , similar to others e.g. ATKC_ECOLI|P03961 potassium-transporting ATPase C chain from Escherichia coli strain K12 (190 aa), FASTA scores: opt: 475, E(): 3.1e-24, (45.7% identity in 186 aa overlap); etc. Belongs to the KdpC family. P9WKF1 No cell wall and cell processes P65211 P9WKF1 GO:0016021,GO:0005886,GO:0006813,GO:0008556,GO:0005524 3.6.3.12 P9WKF1 MSMEG_5394 Mb1060 NC_000962.3 Mycobrowser_v4 CDS 1161297 1162472 . - 0 Rv1039c PPE15 Function unknown PPE family protein PPE15 Rv1039c, (MTCY10G2.10), len: 391 aa. PPE15, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, most similar to Rv2768c|AL008967|MTV002_33 Mycobacterium tuberculosis H37Rv (394 aa), FASTA scores: opt: 1721, E(): 0, (70.4% identity in 398 aa overlap). P9WI31 No PE/PPE Q7D8Y7 P9WI31 P9WI31 MMAR_4452 Mb1068c NC_000962.3 Mycobrowser_v4 CDS 1172386 1172832 . + 0 Rv1049 Rv1049 Involved in transcriptional mechanism Probable transcriptional repressor protein Rv1049, (MTV017.02), len: 148 aa. Probable transcriptional repressor protein, similar to many e.g. P74870 negative regulator of EMR locus EMR from Salmonella typhimurium (149 aa), FASTA scores: opt: 146, E(): 0.0011, (31.6% identity in 95 aa overlap). Contains probable helix-turn-helix motif at aa 58-79 (Score 1495, +4.28 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6Y5H3 No regulatory proteins O53397 I6Y5H3 GO:0006355,GO:0006350,GO:0003700,GO:0005622 I6Y5H3 Mb1078 NC_000962.3 Mycobrowser_v4 CDS 1173945 1174700 . - 0 Rv1051c Rv1051c Function unknown Conserved hypothetical protein Rv1051c, (MTV017.04c), len: 251 aa. Conserved hypothetical protein, similar to LLU36837|U36837.1 protein encoded by Lactococcus lactis plasmid pNP40 (298 aa), FASTA scores: opt: 194, E(): 3.5e-06, (30.3% identity in 155 aa overlap). Contains possible helix-turn-helix motif at aa 197-218 (Score 1097, +2.92 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53399 No conserved hypotheticals O53399 O53399 GO:0000166 O53399 Mb1080c NC_000962.3 Mycobrowser_v4 CDS 1187435 1188001 . + 0 Rv1065 Rv1065 Function unknown Conserved hypothetical protein Rv1065, (MTV017.18), len: 188 aa. Conserved hypothetical protein, some similarity to AL0209|SC4H8_11 hypothetical protein from Streptomyces coelicolor (182 aa), FASTA scores: opt: 156, E(): 0.0011, (31.3% identity in 195 aa overlap). O53413 No conserved hypotheticals O53413 O53413 GO:0017172,GO:0005506,GO:0055114,GO:0046439 O53413 MMAR_4401 MSMEG_5280 Mb1094 NC_000962.3 Mycobrowser_v4 CDS 1156426 1157955 . - 0 Rv1032c trcS Sensor part of the two component regulatory system TRCS/TRCR. Two component sensor histidine kinase TrcS Rv1032c, (MTCY10G2.17), len: 509 aa. TrcS, two component sensor histidine kinase protein (see citations below), similar to YV16_MYCLE|P54883 probable sensor-like histidine kinase from Mycobacterium leprae (443 aa), FASTA scores: opt: 392, E(): 3.8e-18, (31.7% identity in 334 aa overlap). Note that in vitro autophosphorylation of TrcS requires the presence of Mn2+ or Ca2+ as a divalent cation cofactor and subsequent transphosphorylation of TrcR is evident in the presence of TrcS-phosphate and Ca2+. P96368 No regulatory proteins P96368 P96368 GO:0016021,GO:0018106,GO:0007165,GO:0000155,GO:0000160,GO:0005524 2.7.13.3 P96368 ML0260,ML0260c MMAR_4456 MSMEG_2915 Mb1061c NC_000962.3 Mycobrowser_v4 CDS 1160544 1160828 . - 0 Rv1037c esxI Unknown Putative ESAT-6 like protein EsxI (ESAT-6 like protein 1) Rv1037c, (MTCY10G2.12), len: 94 aa. EsxI, ESAT-6 like protein (see citations below), highly similar to Q49946|ES6X_MYCLE|U1756D putative ESAT-6 like protein X from Mycobacterium leprae (95 aa), FASTA scores: opt: 409, E(): 6.3e-23, (64.15% identity in 92 aa overlap); Rv3619c, Rv1198, Rv2346c, etc from Mycobacterium tuberculosis. Strictly identical to P96364|ES61_MYCTU|Rv3619c|MTCY15C10.33|MTCY07H7B.03|MT3721 putative ESAT-6 like protein 1 (94 aa). Belongs to the ESAT6 family. P0DOA6 No cell wall and cell processes P0DOA6 P0DOA6 Mb1066c NC_000962.3 Mycobrowser_v4 CDS 1164572 1165435 . - 0 Rv1041c Rv1041c Possibly required for the transposition of an insertion element. Probable is like-2 transposase Rv1041c, (MTCY10G2.08), len: 287 aa. Probable is like-2 transposase, overlaps MTCY10G2.07. Similar to Q00430|X53945 insertion element IS869 hypothetical protein from Agrobacterium tumefaciens (186 aa), FASTA scores: opt: 173, E(): 0.00016, (40.9% identity in 176 aa overlap). Similar to Rv1150, C-terminal part of transposase of putative Mycobacterium tuberculosis is like-1. MTCY10G2.07 and MTCY10G2.08 are frameshifted with respect to Mycobacterium tuberculosis Q50761 transposase, the 10G2 cosmid sequence appears to be correct. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P96360 No insertion seqs and phages P96360 P96360 GO:0004803,GO:0006313,GO:0003677 P96360 Mb1070c NC_000962.3 Mycobrowser_v4 CDS 1165781 1166806 . - 0 Rv1043c Rv1043c Function unknown Conserved hypothetical protein Rv1043c, (MTCY10G2.06), len: 341 aa. Conserved hypothetical protein similar to AL096872|SC5F7.08 putative lipoate-protein ligase from Streptomyces coelicolor (362 aa), FASTA scores: opt: 206, E(): 1.4e-05, (30.3% identity in 201 aa overlap). Weak similarity to P39668|YYXA_BACSU hypothetical protease from Bacillus subtitis (400 aa), FASTA scores: opt: 159, E(): 0.013, (27.1% identity in 210 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P96358 No conserved hypotheticals P96358 P96358 GO:0004252,GO:0006508 P96358 Mb1072c NC_000962.3 Mycobrowser_v4 CDS 1167053 1167676 . + 0 Rv1044 Rv1044 Function unknown Conserved hypothetical protein Rv1044, (MTCY10G2.05c), len: 207 aa. Conserved hypothetical protein, similar to Mycobacterium tuberculosis hypothetical protein MTCY06G11.02C|P96837 (289 aa), fasta scores: E(): 8.9e-06, (30.7% identity in 150 aa overlap). Some similarity to U36837|LLU36837_1 Lactococcus lactis plasmid pNP40 (287 aa), FASTA scores: opt: 147, E (): 0.0087, (29.7% identity in 91 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P96357 No conserved hypotheticals P96357 P96357 ML1647,ML1647c Mb1073 NC_000962.3 Mycobrowser_v4 CDS 1167673 1168554 . + 0 Rv1045 Rv1045 Unknown Hypothetical protein Rv1045, (MTCY10G2.04c), len: 293 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P96356 No conserved hypotheticals P96356 P96356 P96356 ML1646,ML1646c Mb1074 NC_000962.3 Mycobrowser_v4 CDS 1169423 1170670 . + 0 Rv1047 Rv1047 Possibly required for the transposition of the insertion element IS1081. Probable transposase Rv1047, (MTCY10G2.02c), len: 415 aa. IS1081 transposase, most similar to TRA1_MYCBO|P35882 transposase for insertion sequence element (415 aa), FASTA scores: opt: 2675, E(): 0, (99.8% identity in 415 aa overlap). Contains PS01007 Transposases, Mutator family, signature. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P96354 No insertion seqs and phages P96354 P96354 GO:0004803,GO:0006313,GO:0003677 P96354 Mb1076 NC_000962.3 Mycobrowser_v4 CDS 1171038 1172153 . - 0 Rv1048c Rv1048c Unknown Hypothetical protein Rv1048c, (MTV017.01c-MTCY10G2.01), len: 371 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P96353 No conserved hypotheticals P96353 P96353 Mb1077c NC_000962.3 Mycobrowser_v4 CDS 1187998 1188393 . + 0 Rv1066 Rv1066 Function unknown Conserved hypothetical protein Rv1066, (MTV017.19), len: 131 aa. Conserved hypothetical protein, strong similarity to AL0209|SC4H8.10 hypothetical protein from Streptomyces coelicolor (132 aa), FASTA scores: opt: 429, E(): 5.2e-23, (57.1% identity in 119 aa overlap). O53414 No conserved hypotheticals O53414 O53414 O53414 MMAR_4400 MSMEG_5279 Mb1095 NC_000962.3 Mycobrowser_v4 CDS 1177239 1177373 . + 0 Rv1055 Rv1055 Use for sequence integration. Integrase is necessary for integration of a phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome. Possible integrase (fragment) Rv1055, (MTV017.08), len: 44 aa. Possible integrase (fragment); first 49 aa similar to Rv2309c|MTCY3G12_25|Z79702 hypothetical protein (shows similarity to integrases) from Mycobacterium tuberculosis (151 aa), FASTA scores: opt: 291, E(): 2.2e-16, (74.3% identity in 70 aa overlap); and to L39071|MSGINT_1 integrase from Mycobacterium paratuberculosis (191 aa), FASTA scores: opt: 146, E(): 8.3e-05, (52.1% identity in 48 aa overlap); and to many other integrases or transposases. Shortened since first submission (-34 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53403 No insertion seqs and phages O53403 Mb1084 NC_000962.3 Mycobrowser_v4 CDS 1177628 1178392 . + 0 Rv1056 Rv1056 Function unknown Conserved protein Rv1056, (MTV017.09), len: 254 aa. Conserved protein, some similarity in C-terminal region of Rv0140|MTCI5.14|Z92770 Mycobacterium tuberculosis (126 aa), FASTA scores: opt: 254, E(): 1.2e-10, (43.4% identity in 106 aa overlap); and to Rv1670. C-terminal region is similar to AL035569|SC8D9.02 hypothetical protein from Streptomyces coelicolor (113 aa), FASTA scores: opt: 282, E(): 4.5e-12, (48.0% identity in 100 aa overlap). O53404 No conserved hypotheticals O53404 O53404 O53404 ML0266 MMAR_4414 MSMEG_6499 Mb1085 NC_000962.3 Mycobrowser_v4 CDS 1180684 1182315 . + 0 Rv1058 fadD14 Involved in the fatty acid beta oxidation pathway (degradation). Probable medium chain fatty-acid-CoA ligase FadD14 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv1058, (MTV017.11), len: 543 aa. Probable fadD14, medium-chain fatty-acid-CoA synthetase, highly similar to many e.g. CAC32346.1|AL583945 putative fatty acid CoA ligase from Streptomyces coelicolor (558 aa); N-terminus of NP_419738.1|NC_002696 medium-chain-fatty-acid--CoA ligase from Caulobacter crescentus (1006 aa); Q00594|ALKK_PSEOL medium-chain-fatty-acid--CoA ligase from Pseudomonas oleovorans (546 aa), FASTA scores: opt: 1468, E(): 0, (41.1% identity in 538 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family. O53406 No lipid metabolism O53406 O53406 GO:0008152,GO:0016874 6.2.1.- O53406 MMAR_4412 MSMEG_5291 Mb1087 NC_000962.3 Mycobrowser_v4 CDS 1182391 1183455 . + 0 Rv1059 Rv1059 Function unknown Conserved protein Rv1059, (MTV017.12), len: 354 aa. Conserved protein, similar to Rv0926c|MTCY21C12.20c hypothetical protein from Mycobacterium tuberculosis (358 aa), FASTA scores: opt: 338, E(): 1.4e-14, (33.1% identity in 363 aa overlap). O53407 No conserved hypotheticals O53407 O53407 GO:0008839,GO:0009089,GO:0055114 MMAR_4411 MSMEG_5286 Mb1088 NC_000962.3 Mycobrowser_v4 CDS 1231301 1231990 . + 0 Rv1104 Rv1104 In combination with MTV017.58|Rv1105 catalyzes hydrolysis of several beta-lactam antibiotic PNB esters to the corresponding free acid and PNB alcohol Possible para-nitrobenzyl esterase (fragment) Rv1104, (MTV017.57), len: 229 aa. Possible para-nitrobenzyl esterase (fragment; possibly first part) . Similar to the N-terminal domain of many e.g. P37967|PNBA_BACSU Bacillus subtilis (489 aa), FASTA scores: opt: 715, E(): 0, (53.4% identity in 191 aa overlap). Gene may be inactivated as a frameshift is required to obtain a product continuing in MTV017.58|Rv1105. O53452 No intermediary metabolism and respiration O53452 O53452 GO:0016787 3.1.1.- O53452 Mb1134 NC_000962.3 Mycobrowser_v4 CDS 2000074 2000433 . + 0 Rv1767 Rv1767 Function unknown Conserved protein Rv1767, (MTCY28.33), len: 119 aa. Conserved protein, similar to Q57498|YA53_HAEIN hypothetical protein HI1053 from Haemophilus influenzae (113 aa), FASTA scores: opt: 233, E(): 6.4e-10, (40.0% identity in 90 aa overlap). O06800 No conserved hypotheticals O06800 O06800 O06800 MMAR_2644 Mb1796 NC_000962.3 Mycobrowser_v4 CDS 1186904 1187323 . - 0 Rv1064c lpqV Unknown Possible lipoprotein LpqV Rv1064c, (MTV017.17c), len: 139 aa. Possible lipoprotein LpqV. Has N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. P9WK57 No cell wall and cell processes P9WK57 GO:0005886 MMAR_4402 MSMEG_5281 Mb1093c NC_000962.3 Mycobrowser_v4 CDS 1190757 1192148 . - 0 Rv1068c PE_PGRS20 Function unknown PE-PGRS family protein PE_PGRS20 Rv1068c, (MTV017.21c), len: 463 aa. PE_PGRS20, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002). Similar to AL021897|MTV017_19 Mycobacterium tuberculosis H37Rv (667 aa), FASTA scores: opt: 1875, E(): 0, (55.0% identity in 667 aa overlap). P9WIF9 No PE/PPE O53416 P9WIF9 P9WIF9 MMAR_2097,MMAR_2709,MMAR_2969,MMAR_3199,MMAR_3728,MMAR_4127,MMAR_4377,MMAR_4561,MMAR_5321 Mb1097c NC_000962.3 Mycobrowser_v4 CDS 1192510 1194273 . - 0 Rv1069c Rv1069c Function unknown Conserved protein Rv1069c, (MTV017.22c), len: 587 aa. Conserved protein, hydrophobic regions in N-terminal domain. Similar in part to O07136|B1306.04C B1306.04c protein from Mycobacterium leprae (89 aa), FASTA scores: opt: 229, E(): 1.3e-07, (54.2% identity in 72 aa overlap). O53417 No conserved hypotheticals O53417 O53417 O53417 ML2403 MMAR_4397 MSMEG_5278 Mb1098c NC_000962.3 Mycobrowser_v4 CDS 1194270 1195043 . - 0 Rv1070c echA8 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Probable enoyl-CoA hydratase EchA8 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv1070c, (MTV017.23c), len: 257 aa. Probable echA8, enoyl-CoA hydratase, equivalent to O07137|B1306.05c putative enoyl-CoA hydratase/isomerase from Mycobacterium leprae (257 aa), FASTA scores: opt: 1417, E(): 0, (86.4% identity in 257 aa overlap). Also highly similar to others e.g. NP_106219.1|NC_002678 enoyl CoA hydratase from Mesorhizobium loti (257 aa); L39265|RHMRPST_2 enoyl-CoA hydratase from Rhizobium melilotii (257 aa), FASTA scores: opt: 1100, E(): 0, (66.9% identity in 257 aa overlap); AAK18173.1|AF290950_5|AF290950|FadB1x enoyl-CoA hydratase from Pseudomonas putida (257 aa); etc. Contains PS00166 Enoyl-CoA hydratase/isomerase signature. Belongs to the enoyl-CoA hydratase/isomerase family. P9WNN9 No lipid metabolism 3H81,3PZK P64016 P9WNN9 GO:0006631,GO:0004300 4.2.1.17 P9WNN9 ML2402 MMAR_4396 MSMEG_5277 Mb1099c NC_000962.3 Mycobrowser_v4 CDS 1228683 1229384 . + 0 Rv1100 Rv1100 Function unknown Conserved protein Rv1100, (MTV017.53), len: 233 aa. Conserved protein, slightly similar to Rv1906c|MTCY180.12 hypothetical protein from Mycobacterium tuberculosis (156 aa), FASTA scores: opt: 122, E(): 6.9, (27.4% identity in 135 aa overlap). Equivalent to AL049491|MLCB1222_9 Mycobacterium leprae (257 aa) (63.8% identity in 257 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53448 No conserved hypotheticals O53448 ML1945,ML1945c MMAR_4366 MSMEG_5238 Mb1130 NC_000962.3 Mycobrowser_v4 CDS 1196279 1197115 . + 0 Rv1072 Rv1072 Unknown Probable conserved transmembrane protein Rv1072, (MTV017.25), len: 278 aa. Probable conserved transmembrane protein, equivalent to O07139|B1306.07|Y13803 Protein B1306.07 from Mycobacterium leprae (220 aa), FASTA scores: opt:1032, E(): 0, (75.0% identity in 220 aa overlap); and at the C-terminal end to Q50056|U1740D Mycobacterium leprae (96 aa), FASTA scores: opt: 381, E(): 1.2e-18, (71.6% identity in 81 aa overlap). Similar to Q54192|M80628|STMBLDA_1 transfer RNA-LEU (BLDA) gene and ORF from Streptomyces griseus (293 aa), FASTA scores: opt:558, E(): 4.7e-30, (41.5% identity in 299 aa overlap). O53420 No cell wall and cell processes O53420 O53420 GO:0016021 O53420 ML2400,ML2400c MMAR_4394 MSMEG_5275 Mb1101 NC_000962.3 Mycobrowser_v4 CDS 1199426 1200370 . - 0 Rv1075c Rv1075c Function unknown Conserved exported protein Rv1075c, (MTV017.28c), len: 314 aa. Possibly exported protein, as it contains a N-terminal signal sequence, hydrophobic domain from aa 7-25. Similar to U15183|MLU15183_2 Mycobacterium leprae cosmid B1740 (106 aa), FASTA scores: opt: 207, E(): 1.6e-06, (42.6% identity in 101 aa overlap). Also weak similarity to many glyceraldehyde-3-phosphate dehydrogenases e.g. Q41595|G3PC_TAXBA Taxus baccata (340 aa), FASTA scores: opt: 147, E(): 0.027, (27.5% identity in 189 aa overlap). O53423 No cell wall and cell processes O53423 O53423 GO:0006629,GO:0016788 O53423 ML2398 MMAR_4392 MSMEG_5272 Mb1104c NC_000962.3 Mycobrowser_v4 CDS 1200767 1201660 . + 0 Rv1076 lipU Hydrolyses lipids Possible lipase LipU Rv1076, (MTV017.29), len: 297 aa. Possible lipU, lipase, very similar to several Mycobacterium tuberculosis proteins e.g. Z95390|Rv3487c|MTCY13E12.41c (277 aa), FASTA scores: opt: 1225, E(): 0, (76.0% identity in 246 aa overlap); Rv1426c, etc. Also similar to esterases and lipases of around 300 aa e.g. Q44087 esterase precursor from Acinetobacter lwoffii esterase (303), FASTA scores: opt: 427, E(): 1.9e-21, (32.5% identity in 280 aa overlap). Equivalent to AL035159|MLCB1450 _7 Mycobacterium leprae (335 aa), FASTA scores: opt: 1588, E(): 0, (79.7% identity in 296 aa overlap). O53424 No intermediary metabolism and respiration O53424 O53424 GO:0008152,GO:0016787 3.1.-.- O53424 ML0314,ML0314c MMAR_4391 MSMEG_5271 Mb1105 NC_000962.3 Mycobrowser_v4 CDS 1203313 1204035 . + 0 Rv1078 pra Unknown Probable proline-rich antigen homolog Pra Rv1078, (MTV017.31), len: 240 aa. Probable pra, Proline-rich antigen homolog, equivalent to X65546|MLPRAG_1 proline rich antigen from Mycobacterium leprae (249 aa), FASTA scores: opt: 1162, E(): 3.3e-30, (64.8% identity in 253 aa overlap). Has potential hydrophobic domains. P9WIM7 No conserved hypotheticals O53426 P9WIM7 ML2395,ML2395c MMAR_4389 MSMEG_5266 Mb1107 NC_000962.3 Mycobrowser_v4 CDS 1229391 1230548 . - 0 Rv1101c Rv1101c Unknown Conserved membrane protein Rv1101c, (MTV017.54c), len: 385 aa. Conserved membrane protein, shows some similarity to other bacterial proteins e.g. P77406|PERM_ECOLI putative permease perm from Escherichia coli (353 aa), FASTA scores: opt: 287, E(): 8.8e-12, (24.9% identity in 349 aa overlap). P9WFM3 No cell wall and cell processes O53449 P9WFM3 GO:0005886,GO:0016021 P9WFM3 MMAR_4364 MSMEG_5237 Mb1131c NC_000962.3 Mycobrowser_v4 CDS 1205304 1205798 . - 0 Rv1080c greA Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent trancript by cleavage factors such as GREA or GREB allows the resumption of elongation from the new 3'terminus. GREA releases sequences of 2 to 3 nucleotides Probable transcription elongation factor GreA (transcript cleavage factor GreA) Rv1080c, (MTV017.33c), len: 164 aa. Probable greA, transcription elongation factor G, closest to P46808|GREA_MYCLE transcription elongation factor G from Mycobacterium leprae (202 aa), FASTA scores: opt: 1005, E(): 0, (94.5% identity in 164 aa overlap); and similar to many e.g. P21346|GREA_ECOLI from Escherichia coli (158 aa), FASTA scores: opt: 257, E(): 5.7e-10, (37.2% identity in 148 aa overlap); etc. Contains two PS00829 and one PS00830 Prokaryotic transcription elongation factors signatures 1 and 2, respectively. Belongs to the GREA/GREB family. P9WMT9 No information pathways P64279 P9WMT9 GO:0006355,GO:0006350,GO:0003711,GO:0003677 P9WMT9 ML2393 MMAR_4387 MSMEG_5263 Mb1109c NC_000962.3 Mycobrowser_v4 CDS 1205984 1206418 . - 0 Rv1081c Rv1081c Unknown Probable conserved membrane protein Rv1081c, (MTV017.34c), len: 144 aa. Probable conserved membrane protein, with hydrophobic stretch from aa 26 - 48, highly similar to NP_302548.1|NC_002677 conserved membrane protein from Mycobacterium leprae. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). O53429 No cell wall and cell processes O53429 ML2392 MSMEG_5262 Mb1110c NC_000962.3 Mycobrowser_v4 CDS 1207383 1207649 . + 0 Rv1083 Rv1083 Function unknown Conserved hypothetical protein Rv1083, (MTV017.36), len: 88 aa. Conserved hypothetical protein, similar to U15183|MLU15183_9 hypothetical protein from Mycobacterium leprae (167 aa), FASTA scores: opt: 332, E(): 1.2e-13, (58.4% identity in 101 aa overlap). Hydrophobic domain aa 25-43. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53431 No conserved hypotheticals O53431 ML2390,ML2390c MMAR_4384 MSMEG_5260 Mb1112 NC_000962.3 Mycobrowser_v4 CDS 1219248 1220186 . - 0 Rv1092c coaA Coenzyme A (CoA) biosynthesis [catalytic activity: ATP + pantothenate = ADP + D-4'- phosphopantothenate.] Probable pantothenate kinase CoaA (pantothenic acid kinase) Rv1092c, (MTV017.45c), len: 312 aa. Probable coaA, pantothenate kinase, similar to many e.g. P15044|COAA_ECOLI Escherichia coli (316 aa), FASTA scores :opt: 1079, E(): 0, (52.7% identity in 311 aa overlap). Equivalent to AL049491|MLCB1222_17 Mycobacterium leprae (312 aa) (93.6% identity in 312 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the pantothenate kinase family. P9WPA7 No intermediary metabolism and respiration 2GES,2GET,2GEU,2GEV,2ZS7,2ZS8,2ZS9,2ZSA,2ZSB,2ZSD,2ZSE,2ZSF,3AEZ,3AF0,3AF1,3AF2,3AF3,3AF4,3AVO,3AVP,3AVQ P63810 P9WPA7 GO:0015937,GO:0005737,GO:0004594,GO:0005524 2.7.1.33 P9WPA7 ML1954 MMAR_4376 MSMEG_5252 Mb1122c NC_000962.3 Mycobrowser_v4 CDS 1207636 1209657 . + 0 Rv1084 Rv1084 Function unknown Conserved protein Rv1084, (MTV017.37), len: 673 aa. Conserved protein, similar to P37512|YYAL_BACSU hypothetical protein from Bacillus subtilis (689 aa), FASTA scores: opt: 1063, E() : 0, (36.5% identity in 696 aa overlap); AE0009|AE000983_10 Archaeoglobus fulgidus section 1 (642 aa), FASTA scores: opt: 1018, E(): 0, (37.2% identity in 600 aa overlap). Also similar to AE001938|AE001938_9 Deinococcus radiodurans (690 aa), FASTA scores: opt: 1097, E(): 0, (41.6% identity in 694 aa overlap). O53432 No conserved hypotheticals O53432 O53432 GO:0003824 O53432 ML2389,ML2389c MMAR_4383 MSMEG_5259 Mb1113 NC_000962.3 Mycobrowser_v4 CDS 1209756 1210484 . - 0 Rv1085c Rv1085c Unknown, but supposedly involved in virulence Possible hemolysin-like protein Rv1085c, (MTV017.38c), len: 242 aa. Possible hemolysin-like protein, integral membrane protein, similar to many hemolysins, and hypothetical proteins e.g. U28375|ECU28375_49 Hypothetical protein from Escherichia coli (219 aa), FASTA scores: opt: 308, E(): 7.5e-15, (30.6% identity in 180 aa overlap); AE0011|HIAE001124_2 Hypothetical protein from Borrelia burgdorferi (233 aa), FASTA scores: opt: 305, E(): 1.3e-14, (25.6% identity in 203 aa overlap). Also weakly similar to HLY3_BACCE|P54176 haemolysin from Bacillus cereus (219 aa), FASTA scores: opt: 247, E(): 8.7e-12, (27.5% identity in 171 aa overlap). Also similar to AE002027|AE002027_8 probable hemolysin from Deinococcus radiodurans (219 aa), FASTA scores: opt: 354, E(): 1.8e-16, (31.1% identity in 219 aa overlap). P9WFN7 No virulence, detoxification, adaptation P67157 P9WFN7 GO:0016021,GO:0005886,GO:0019835 ML2466,ML2466c MMAR_4381 MSMEG_5257 Mb1114c NC_000962.3 Mycobrowser_v4 CDS 1220574 1221854 . + 0 Rv1093 glyA1 Interconversion of serine and glycine. Key enzyme in the biosynthesis of purines, lipids, hormones and other components [catalytic activity: 5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine.] Serine hydroxymethyltransferase 1 GlyA1 Rv1093, (MTV017.46), len: 426 aa. glyA1, serine hydroxymethyltransferase 1, equivalent to AL049491|MLCB1222_16 from Mycobacterium leprae (426 aa), FASTA score: (89.9 % identity in 426 aa overlap). Also similar to many e.g. P34895|GLYA_HYPME hyphomicrobium methylovorum (434 aa), FASTA scores: opt: 1492, E(): 0, (56.8% identity in 419 aa overlap); etc. Belongs to the ShmT family. Note that previously known as glyA. P9WGI9 No intermediary metabolism and respiration 1LXB,3H7F O53441 P9WGI9 GO:0005737,GO:0004372,GO:0006544,GO:0006730,GO:0030170,GO:0006563 2.1.2.1 P9WGI9 ML1953,ML1953c MMAR_4375 Mb1123 NC_000962.3 Mycobrowser_v4 CDS 1232311 1232826 . + 0 Rv1105 Rv1105 In combination with MTV017.57|Rv1104 catalyzes hydrolysis of several beta-lactam antibiotic PNB esters to the corresponding free acid and PNB alcohol Possible para-nitrobenzyl esterase (fragment) Rv1105, (MTV017.58), len: 171 aa. Possible para-nitrobenzyl esterase (fragment; possibly second part) . Similar to C-terminal domain of many e.g. P71048 para-nitrobenzyl esterase from Bacillus subtilis (489 aa), FASTA scores: opt: 248, E(): 2.7e-10, (32.3% identity in 167 aa overlap). Gene may be inactivated as a frameshift is required to obtain a product continuing from MTV017.57|Rv1104. Start changed since first submission. O53453 No intermediary metabolism and respiration O53453 O53453 GO:0016787 3.1.1.- O53453 Mb1135 NC_000962.3 Mycobrowser_v4 CDS 1696727 1697422 . - 0 Rv1507c Rv1507c Function unknown Conserved protein Rv1507c, (MTCY277.29c), len: 231 aa. Conserved protein. Similar to AJ007747|BBR007747_6 Hypothetical protein BbLPS1.06 from Bordetella bronchiseptica cosmid (239 aa), FASTA scores: opt: 362, E(): 1.3e-17, (30.8% identity in 221 aa overlap). P9WLW5 No conserved hypotheticals P71786 P9WLW5 P9WLW5 MMAR_2325 NC_000962.3 Mycobrowser_v4 CDS 2139741 2140148 . + 0 Rv1891 Rv1891 Function unknown Conserved protein Rv1891, (MTCY180.27c), len: 135 aa. Conserved protein. Equivalent to MLCB561.09|AL049571 hypothetical protein from Mycobacterium leprae (134 aa), FASTA scores: opt: 800, E(): 0, (79.7% identity in 133 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O07741 No conserved hypotheticals O07741 ML2023,ML2023c MMAR_2784 MSMEG_3686 Mb1924 NC_000962.3 Mycobrowser_v4 CDS 1214040 1214360 . + 0 Rv1087A Rv1087A Function unknown Conserved hypothetical protein Rv1087A, len: 106 aa (fragment). Conserved hypothetical protein, highly similar to C-terminus of near ORF O53434|YA86_MYCTU|Rv1086|MT1118|MTV017.39 short (C15) chain Z-isoprenyl diphosphate synthase from Mycobacterium tuberculosis (262 aa), FASTA scores: opt: 200, E(): 1.1e-06, (57.9% identity in 76 aa overlap). L7N654 No cell wall and cell processes Q8VK75 L7N654 GO:0016765 L7N654 NC_000962.3 Mycobrowser_v4 CDS 1214769 1215131 . + 0 Rv1089 PE10 Function unknown PE family protein PE10 Rv1089, (MTV017.42), len: 120 aa. PE10, Member of the Mycobacterium tuberculosis PE family of glycine-rich proteins (see citation below). Partial ORF that appears to be frameshifted continuation of Rv1088|MTV017.41. Sequence has been checked and appears correct. Similar to Z95555|MTCY06F7_4 Mycobacterium tuberculosis cosmid (401 aa), FASTA scores: opt:126, E(): 2, (29.6% identity in 125 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L0T5T4 No PE/PPE L0T5T4 Mb1118 NC_000962.3 Mycobrowser_v4 CDS 1215517 1215621 . + 0 Rv1089A celA2a The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) exocellobiohydrolases that cut the dissaccharide cellobiose from the nonreducing end of the cellulose polymer chain; (3) beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose [catalytic activity:endohydrolysis of 1,4-beta-D-glucosidic linkages in cellulose]. Probable cellulase CelA2a (endo-1,4-beta-glucanase) (endoglucanase) (carboxymethyl cellulase) Rv1089A, len: 34 aa. Probable celA2a, first part of cellulase (endoglucanase), similar to N-terminus of others. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q79FS6 No intermediary metabolism and respiration Q79FS6 GO:0008152,GO:0008810 3.2.1.4 Q79FS6 Mb1119 NC_000962.3 Mycobrowser_v4 CDS 1215599 1216054 . + 0 Rv1090 celA2b The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) exocellobiohydrolases that cut the dissaccharide cellobiose from the nonreducing end of the cellulose polymer chain; (3) beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose [catalytic activity:endohydrolysis of 1,4-beta-D-glucosidic linkages in cellulose]. Probable cellulase CelA2b (endo-1,4-beta-glucanase) (endoglucanase) (carboxymethyl cellulase) Rv1090, (MTV017.43), len: 151 aa. Probable celA2b, second part of cellulase (endoglucanase), similar to C-terminus of others e.g. O08468 cellulase CEL2 from Streptomyces halstedi (377 aa), FASTA scores: opt: 554, E(): 1.2e-30, (52.0% identity in 152 aa overlap); etc. Gene appears to have been inactivated by frameshift mutations but no errors could be found that would account for this. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53438 No intermediary metabolism and respiration O53438 O53438 GO:0000272,GO:0008810 3.2.1.4 O53438 Mb1120 NC_000962.3 Mycobrowser_v4 CDS 1216469 1219030 . + 0 Rv1091 PE_PGRS22 Function unknown PE-PGRS family protein PE_PGRS22 Rv1091, (MTV017.44), len: 853 aa. PE_PGRS22, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below). Similar to Rv1087|AL021897|MTV017_39 Mycobacterium tuberculosis H37Rv (767 aa), FASTA scores: opt: 2819, E(): 0, (60.0% identity in 860 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q79FS5 No PE/PPE Q79FS5 Q79FS5 Q79FS5 MMAR_0837,MMAR_4223,MMAR_4224 Mb1121 NC_000962.3 Mycobrowser_v4 CDS 1230660 1230971 . - 0 Rv1102c mazF3 Sequence-specific mRNA cleavage Toxin MazF3 Rv1102c, (MTV017.55c), len: 103 aa. MazF3, toxin, part of toxin-antitoxin (TA) operon with Rv1103c (See Pandey and Gerdes, 2005; Zhu et al., 2006), similar to Mycobacterium tuberculosis hypothetical protens e.g. Rv1942c|MTCY9F9_22 (109 aa), FASTA scores: opt: 158, E(): 3.6e-05, (33.3% identity in 93 aa overlap); Rv0659c|MTCI376_17 (102aa), opt: 140, E(): 0.00072, (40.6% identity in 69aa overlap); and Rv1495. P9WIH9 No virulence, detoxification, adaptation O53450 P9WIH9 GO:0003677 P9WIH9 ML2576,ML2576c Mb1132c NC_000962.3 Mycobrowser_v4 CDS 2140165 2140476 . + 0 Rv1892 Rv1892 Unknown Probable membrane protein Rv1892, (MTCY180.26c), len: 103 aa. Probable membrane protein. O07740 No cell wall and cell processes O07740 O07740 MMAR_2785 MSMEG_3523 Mb1925 NC_000962.3 Mycobrowser_v4 CDS 1222997 1224298 . + 0 Rv1095 phoH2 Unknown Probable PHOH-like protein PhoH2 (phosphate starvation-inducible protein PSIH) Rv1095, (MTV017.48), len: 433 aa. Probable phoH2, phoH-like protein (phosphate starvation-induced protein), probably ATP-binding protein. Equivalent to AL049491 MLCB1222_14 Mycobacterium leprae (433 aa) (92.8% identity in 432 aa overlap). Similar to many proteins described as PhoH-like e.g. Z97025|BSZ97025_12 Bacillus subtilis (442 aa), FASTA scores: opt: 605, E(): 0, (40.1% identity in 444 aa overlap); or Mycobacterium tuberculosis Rv2368c|O05830|PHOL_MYCTU Mycobacterium tuberculosis (352 aa), FASTA scores: opt: 390, E(): 4e-19, (31.5% identity in 241 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the PhoH family. O53443 No intermediary metabolism and respiration O53443 O53443 GO:0005524 O53443 ML1951,ML1951c MMAR_4372 MSMEG_5247 Mb1125 NC_000962.3 Mycobrowser_v4 CDS 1224385 1225260 . + 0 Rv1096 Rv1096 Probably involved in carbohydrate degradation. May hydrolyse the glycosidic bond between two or more carbohydrates or between a carbohydrate and a non-carbohydrate moiety. Possible glycosyl hydrolase Rv1096, (MTV017.49), len: 291 aa. Possible glycosyl hydrolase, possibly deacetylase or esterase. Equivalent to AL049491|MLCB1222_13 Mycobacterium leprae (291 aa) (81.3% identity in 289 aa overlap). Similar at the C-terminus to enzymes involved in carbohydrate degradation including Z99110|BSUB0007_92 endo-1,4-beta-xylanase homolog yjeA from Bacillus subtilis (467 aa), FASTA scores: opt: 418, E(): 2.6e-17, (38.6% identity in 184 aa overlap); M64552|STMXLNB_2 acetyl-xylan esterase from Streptomyces lividans (335 aa), FASTA scores: opt: 371, E(): 1.1e-14, (31.6% identity in 237 aa overlap); NP_345933.1|NC_003028 peptidoglycan N-acetylglucosamine deacetylase a from Streptococcus pneumoniae (463 aa); etc. Has possible N-terminal signal sequence with TMhelix at aa 13-31. O53444 No intermediary metabolism and respiration O53444 O53444 GO:0016810,GO:0005975 3.-.-.- O53444 ML1950,ML1950c MMAR_4371 Mb1126 NC_000962.3 Mycobrowser_v4 CDS 1230971 1231291 . - 0 Rv1103c mazE3 Function unknown Possible antitoxin MazE3 Rv1103c, (MTV017.56c), len: 106 aa. Possible mazE3, antitoxin, part of toxin-antitoxin (TA) operon with Rv1102c (See Pandey and Gerdes, 2005; Zhu et al., 2006). Note that Zhu et al., 2006 identifies a different amino acid sequence as the possible antitoxin to Rv1102c. Similar to part of Mycobacterium tuberculosis hypothetical protein Rv2472|AL021246|MTV008_27 Mycobacterium tuberculosis (97 aa), FASTA score: opt: 135, E(): 0.0091, (45.8% identity in 72 aa overlap). O53451 No virulence, detoxification, adaptation O53451 ML2577,ML2577c Mb1133c NC_000962.3 Mycobrowser_v4 CDS 1235457 1236095 . - 0 Rv1109c Rv1109c Function unknown Conserved protein Rv1109c, (MTV017.62c), len: 212 aa. Conserved protein. Equivalent to AL049491|MLCB1222_4 hypothetical protein from Mycobacterium leprae (205 aa) (68.1% identity in 213 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WM59 No conserved hypotheticals P9WM59 ML1939 MMAR_4356 MSMEG_5225 Mb1139c NC_000962.3 Mycobrowser_v4 CDS 1239416 1239613 . + 0 Rv1113 vapB32 Unknown Possible antitoxin VapB32 Rv1113, (MTCY22G8.02), len: 65 aa. Possible vapB32, antitoxin, part of toxin-antitoxin (TA) operon with Rv1114, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. Rv2758c|AL00896 7|MTV002.23 (88 aa) FASTA scores: opt: 97, E(): 0.86, (33.3% identity in 69 aa overlap). Part of family including Rv2871, Rv1241, Rv2132, Rv3321c, etc. P9WJ33 No virulence, detoxification, adaptation O06565 P9WJ33 P9WJ33 Mb1143 NC_000962.3 Mycobrowser_v4 CDS 1239610 1239984 . + 0 Rv1114 vapC32 Unknown Possible toxin VapC32. Contains PIN domain. Rv1114, (MTCY22G8.03), len: 124 aa. Possible vapC32, toxin, part of toxin-antitoxin (TA) operon with Rv1113, contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. Rv1561 and Rv2010. P9WF73 No virulence, detoxification, adaptation O06566 P9WF73 P9WF73 Mb1144 NC_000962.3 Mycobrowser_v4 CDS 1253074 1254534 . - 0 Rv1129c Rv1129c Involved in transcriptional mechanism Probable transcriptional regulator protein Rv1129c, (MTCY22G8.18c), len: 486 aa. Possible transcriptional regulator protein, similar to Rv0465c|MTV038.09c Mycobacterium tuberculosis (474 aa), FASTA scores: E(): 0, (47.4% identity in 468 aa overlap). Helix turn helix motif present from aa 32-53. O06581 No regulatory proteins O06581 O06581 GO:0043565 O06581 MMAR_1378 MSMEG_6643 Mb1160c NC_000962.3 Mycobrowser_v4 CDS 1227596 1228684 . - 0 Rv1099c glpX Involved in gluconeogenesis [catalytic activity: fructose-1,6-bisphosphate + H2O = D-fructose-6-phosphate + phosphate] Fructose 1,6-bisphosphatase GlpX Rv1099c, (MTV017.52c), len: 362 aa. glpX, class II fructose 1,6-bisphosphatase (See Movahedzadeh et al., 2004), highly similar to P44811|GLPX_HAEIN GLPX protein homolog (believed to be involved in glycerol metabolism) (333 aa), FASTA scores: opt: 763, E():0, (46.2% identity in 327 aa overlap); and Q03224|YWJI_BACSU hypothetical protein from Bacillus subtilis (321aa), FASTA scores: opt: 1092, E(): 0, (52.1% identity in 313 aa overlap). Equivalent to AL049491|MLCB1222_10 Mycobacterium leprae (355 aa), (93.0% identity in 328 aa overlap). N-terminus extended since first submission (previously 328 aa). P9WN21 No intermediary metabolism and respiration O53447 P9WN21 GO:0006071 P9WN21 ML1946 MMAR_4367 MSMEG_5239 Mb1129c NC_000962.3 Mycobrowser_v4 CDS 1233966 1234223 . - 0 Rv1107c xseB Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides [catalytic activity: exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphomononucleotides.] Probable exodeoxyribonuclease VII (small subunit) XseB (exonuclease VII small subunit) Rv1107c, (MTV017.60c), len: 85 aa. Probable xseB, exonuclease VII small subunit (see citation below). Equivalent to AL049491|MLCB1222_6 Mycobacterium leprae (87 aa) (77.9% identity in 68 aa overlap). Similar to P43914|EX7S_HAEIN exodeoxyribonuclease small subunit from H. influenzae (84 aa), FASTA scores: opt: 126, E(): 0.006, (37.3% identity in 67 aa overlap); and P22938|EX7S_ECOLI exodeoxyribonuclease small subunit from Escherichia coli (79 aa), FASTA scores: opt: 125, E(): 0.0067, (39.7% identity in 58 aa overlap). Belongs to the XseB family. P9WF29 No information pathways P67456 P9WF29 GO:0005737,GO:0008855,GO:0009318,GO:0006308 3.1.11.6 P9WF29 ML1941 MMAR_4358 MSMEG_5227 Mb1137c NC_000962.3 Mycobrowser_v4 CDS 1234213 1235460 . - 0 Rv1108c xseA Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides [catalytic activity: exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphomononucleotides] Probable exodeoxyribonuclease VII (large subunit) XseA (exonuclease VII large subunit) Rv1108c, (MTV017.61c), len: 415 aa. Probable xseA, exodeoxyribonuclease VII large subunit (see Mizrahi & Andersen 1998). Equivalent to AL049491|MLCB1222_5 Mycobacterium leprae (428 aa) (81.5% identity in 411 aa overlap). Similar to many e.g. P04994|EX7L_ECOLI exodeoxyribonuclease large subunit from Escherichia coli (456 aa), FASTA scores: opt: 581, E(): 1.6 e-30, (30.8% identity in 425 aa overlap); also similar to the exodeoxyribonuclease in Bacillus subtilis, H. influenzae and H. pylori. Belongs to the XseA family. P9WF31 No information pathways P67447 P9WF31 GO:0005737,GO:0008855,GO:0009318,GO:0006308 3.1.11.6 P9WF31 ML1940 MMAR_4357 MSMEG_5226 Mb1138c NC_000962.3 Mycobrowser_v4 CDS 1237209 1238192 . - 0 Rv1111c Rv1111c Function unknown Conserved hypothetical protein Rv1111c, (MTV017.64c), len: 327 aa. Conserved hypothetical protein, N-terminal domain is hydrophobic, C-terminal half is very rich in Arg. Equivalent to AL049491|MLCB1222_2 hypothetical protein from Mycobacterium leprae (379 aa) (46.0% identity in 374 aa overlap). Start changed since first submission. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O86351 No conserved hypotheticals O86351 ML1937 MMAR_4354 MSMEG_5223 Mb1141c NC_000962.3 Mycobrowser_v4 CDS 1238255 1239328 . + 0 Rv1112 Rv1112 Unknown Probable GTP binding protein Rv1112, (MTCY22G8.01-MTV017.65), len: 357 aa. Probable GTP binding protein, similar to YCHF_HAEIN|P44681 probable gtp-binding protein (362 aa), FASTA scores: opt: 1189, E(): 0, (52.7% identity in 357 aa overlap). Equivalent to AL049491|MLCB1222_1 hypothetical protein from Mycobacterium leprae (356 aa) (85.9% identity in 354 aa overlap0. Contains PS00017 ATP/GTP-binding site motif A (P-loop). O53459 No conserved hypotheticals O53459 O53459 GO:0005622,GO:0005525 O53459 ML1936,ML1936c MMAR_4353 MSMEG_5222 Mb1142 NC_000962.3 Mycobrowser_v4 CDS 1243707 1245107 . + 0 Rv1121 zwf1 Involved in pentose phosphate pathway (first step) [catalytic activity: D-glucose 6-phosphate + NADP(+) = D-glucono-1,5-lactone 6-phosphate + NADPH.] Probable glucose-6-phosphate 1-dehydrogenase Zwf1 (G6PD) Rv1121, (MTCY22G8.10), len: 466 aa. Probable zwf1, glucose-6-phosphate 1-dehydrogenase, highly similar to many e.g. G6PD_E COLI|P22992 Escherichia coli (491 aa), FASTA scores: opt: 642, E(): 0, (35.8% identity in 478 aa overlap). Mycobacterium tuberculosis has two genes for ZWF, this one is highly divergent. Belongs to the glucose-6-phosphate dehydrogenase family. Note that previously known as zwf. Nucleotide position 1244700 in the genome sequence has been corrected, T:C resulting in L332L. P9WN71 No intermediary metabolism and respiration P0A586 P9WN71 GO:0006006,GO:0004345,GO:0055114 1.1.1.49 P9WN71 ML0943 MMAR_4339 Mb1152 NC_000962.3 Mycobrowser_v4 CDS 1245129 1246151 . + 0 Rv1122 gnd2 Involved in hexose monophosphate shunt (pentose phosphate pathway). [catalytic activity: 6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH] Probable 6-phosphogluconate dehydrogenase, decarboxylating Gnd2 Rv1122, (MTCY22G8.11), len: 340 aa. Probable gnd2, 6-phosphogluconate dehydrogenase, decarboxylating, highly similar to Q53917 6-phosphogluconate dehydrogenase from Streptomyces coelicolor (291 aa), fasta scores: opt: 431, E(): 2.2e-20, (44.5% identity in 335 aa overlap). Also similar to Rv1844c|MTCY359.29|gnd1 probable 6-phosphogluconate dehydrogenase from Mycobacterium tuberculosis (485 aa), FASTA score: (33.0% identity in 351 aa overlap). Note that Rv1844c|MTCY359.29|gnd1 is most similar to gnd's from Gram negative organisms, while gnd2 is most similar to gnd's from Gram positive organisms. Belongs to the 6-phosphogluconate dehydrogenase family. O06574 No intermediary metabolism and respiration O06574 O06574 GO:0055114,GO:0006098,GO:0004616,GO:0050661 1.1.1.44 O06574 ML0944 MMAR_4338 MSMEG_0002 Mb1153 NC_000962.3 Mycobrowser_v4 CDS 1246144 1247052 . - 0 Rv1123c bpoB Supposedly involved in detoxification reactions. Possible peroxidase BpoB (non-haem peroxidase) Rv1123c, (MTCY22G8.12c), len: 302 aa. Possible bpoB, peroxidase (non-haem peroxidase), with some similarity to a range of enzymes from several organisms including: DEH1_MORSP|Q01398 haloacetate dehalogenase from Moraxella sp. (294 aa), FASTA scores: opt: 201, E(): 2.1e-06, (35.8% identity in 134 aa overlap); and BPA1_STRAU|P33912 non-haem bromoperoxidase bpo-a1 from Streptomyces aureofaciens (274 aa), FASTA scores: opt: 187, E(): 1.6e-05, (23.1% identity in 281 aa overlap). Similar to several other Mycobacterium tuberculosis proteins, probable epoxide hydrolases and non-heme bromoperoxidases e.g. Rv1938, Rv3617, Rv3473c, Rv3171c, etc. Contains PS00216 Sugar transport proteins signature 1. O06575 No virulence, detoxification, adaptation O06575 O06575 GO:0004601,GO:0055114 1.11.1.- O06575 ML0945,ML0945c MSMEG_5209 Mb1154c NC_000962.3 Mycobrowser_v4 CDS 2156706 2157299 . - 0 Rv1910c Rv1910c Unknown Probable exported protein Rv1910c, (MTCY180.08), len: 197 aa. Possible exported protein, very similar to upstream ORF MTCY180.07 (201 aa), FASTA score: E(): 0, (64.0% identity in 200 aa overlap). Also similar to Q9Z729|Y877_CHLPN protein CPN0877 from Chlamydophila pneumoniae (150 aa). Predicted to be an outer membrane protein (See Song et al., 2008). P9WFN5 No cell wall and cell processes P67222 P9WFN5 P9WFN5 MSMEG_6851 Mb1945c NC_000962.3 Mycobrowser_v4 CDS 1248082 1249326 . + 0 Rv1125 Rv1125 Function unknown Conserved hypothetical protein Rv1125, (MTCY22G8.14), len: 414 aa. Conserved hypothetical protein. Similar to AL133278|SCM11.13 hypothetical protein from Streptomyces coelicolor (446 aa), FASTA scores: opt: 182, E(): 0.0005, (28.1% identity in 437 aa overlap). O06577 No conserved hypotheticals O06577 ML0952 MMAR_4333 MSMEG_5205 Mb1156 NC_000962.3 Mycobrowser_v4 CDS 1249330 1249935 . - 0 Rv1126c Rv1126c Function unknown Conserved protein Rv1126c, (MTCY22G8.15c), len: 201 aa. Conserved protein, similar in N-terminus to O05567|MLCB33.17 hypothetical protein from Mycobacterium leprae (141 aa), FASTA scores: opt: 332, E(): 1.4e-23, (58.4% identity in 101 aa overlap). O06578 No conserved hypotheticals O06578 O06578 ML0954,ML0954c MMAR_4332 Mb1157c NC_000962.3 Mycobrowser_v4 CDS 1251617 1252972 . - 0 Rv1128c Rv1128c Unknown Conserved hypothetical protein Rv1128c, (MTCY22G8.17c), len: 451 aa. Conserved hypothetical protein, in REP13E12 degenerate repeat, highly similar to several Mycobacterium tuberculosis proteins in REP13E12 repeats e.g. Rv1148c, Rv1945, Rv3467, etc. P9WM57 No insertion seqs and phages O06580 P9WM57 P9WM57 MMAR_1523,MMAR_3121 MSMEG_2663,MSMEG_6573 Mb1159c NC_000962.3 Mycobrowser_v4 CDS 1254555 1256135 . + 0 Rv1130 prpD Involved in methyl citrate cycle Possible methylcitrate dehydratase PrpD Rv1130, (MTCY22G8.19), len: 526 aa. Possible prpD, methylcitrate dehydratase (MCD), some similarity to AP000063|AP000063_192 hypothetical protein from Aeropyrum pernix (479 aa), FASTA scores: opt: 717, E(): 0, (34.3% identity in 443 a a overlap), and to PRPD_ECOLI|P77243 prpd protein from Escherichia coli (483aa), FASTA scores: opt: 234, E(): 3.3e-08, (27.0% identity in 429 aa overlap). Predicted possible vaccine candidate (See Zvi et al., 2008). O06582 No intermediary metabolism and respiration O06582 O06582 GO:0019543,GO:0047547 O06582 MMAR_1379 MSMEG_6645 Mb1161 NC_000962.3 Mycobrowser_v4 CDS 1264314 1264556 . + 0 Rv1135A Rv1135A Function unknown, but involved in lipid degradation [catalytic activity: 2 acetyl-CoA = CoA + acetoacetyl-CoA]. Possible acetyl-CoA acetyltransferase (acetoacetyl-CoA thiolase) Rv1135A, len: 80 aa. Possible acetyl-CoA acetyltransferase (possible gene fragment), highly similar to other acetyl-CoA acetyltransferases e.g. C-terminal part of Rv3556c|Z92774|MTCY6G11_2|MTCY06G11.03|fadA6 acetyl-CoA acetyltransferase from Mycobacterium tuberculosis (386 aa), FASTA scores: opt: 219, E(): 5.7e-09, (63.6% identity in 55 aa overlap). L7N682 No lipid metabolism Q8VK64 L7N682 GO:0008152,GO:0003985 2.3.1.9 L7N682 Mb1167 NC_000962.3 Mycobrowser_v4 CDS 1266485 1266985 . - 0 Rv1139c Rv1139c Function unknown Conserved hypothetical membrane protein Rv1139c, (MTCI65.06c), len: 166 aa. Conserved hypothetical membrane protein. Highly similar to P54158|YBPQ_BACSU hypothetical Bacillus subtilis protein, YBPQ (168 aa), FASTA scores: opt: 446, E(): 2.2e-26, (38.4% identity in 164 aa overlap). Some similarity to Mycobacterium tuberculosis hypothetical proteins, Rv0740, Rv0750. O06539 No cell wall and cell processes O06539 O06539 GO:0016021,GO:0004671,GO:0006481 O06539 ML0966,ML0966c MMAR_4314 MSMEG_0809 Mb1171c NC_000962.3 Mycobrowser_v4 CDS 1257325 1259055 . + 0 Rv1132 Rv1132 Unknown Conserved membrane protein Rv1132, (MTCY22G8.21), len: 576 aa. Conserved membrane protein, similar to O06827|Rv1431|MTCY493.23C membrane protein from Mycobacterium tuberculosis (589 aa), fasta scores: opt: 1811, E(): 0, (48.2% identity in 585 aa overlap). O06583 No cell wall and cell processes O06583 O06583 O06583 ML0960 MSMEG_5202 Mb1163 NC_000962.3 Mycobrowser_v4 CDS 1262272 1264128 . - 0 Rv1135c PPE16 Function unknown PPE family protein PPE16 Rv1135c, (MTCI65.02c), len: 618 aa. PPE16, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins. Similar to Rv2356c (59.6% identity in 627 aa overlap); etc.. Predicted to be an outer membrane protein (See Song et al., 2008). P9WI29 No PE/PPE Q79FS0 P9WI29 P9WI29 Mb1166c NC_000962.3 Mycobrowser_v4 CDS 1267347 1268195 . + 0 Rv1140 Rv1140 Function unknown Probable integral membrane protein Rv1140, (MTCI65.07), len: 282 aa. Probable integral membrane protein. Weak similarity in C-terminus to hypothetical Escherichia coli proteins YPRA and YPRB, possibly membrane-bound e.g. YPRA_ECOLI hypothetical 24.3 kDa protein (URF 1) (217 aa), FASTA scores: opt: 166, E(): 0.00062, (31.0% identity in 158 aa overlap). O06540 No cell wall and cell processes O06540 O06540 GO:0016020 ML0968 MMAR_4311 MSMEG_5188 Mb1172 NC_000962.3 Mycobrowser_v4 CDS 1268203 1269009 . - 0 Rv1141c echA11 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Probable enoyl-CoA hydratase EchA11 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv1141c, (MTCI65.08c), len: 268 aa. Probable echA11, enoyl-CoA hydratase, similar to others e.g. P24162|ECHH_RHOCA probable enoyl-CoA hydratase from Rhodobacter capsulatus(257 aa); CAA66096.1|X97452 enoyl-CoA isomerase from Escherichia coli (262 aa), FASTA scores: opt: 513, E():1e-25, (36.1% identity in 249 aa overlap); etc. Also similarity with naphthoate synthases. Also highly similar to downstream ORF Rv1142c|MTCI65.09|echA10 probable enoyl-CoA hydratase from Mycobacterium tuberculosis (268 aa), FASTA scores: opt: 1225, E(): 0, (72.3% identity in 267 aa overlap). O06541 No lipid metabolism O06541 O06541 GO:0016853,GO:0008152,GO:0004300 4.2.1.17 O06541 MSMEG_5185 Mb1173c NC_000962.3 Mycobrowser_v4 CDS 1270062 1271144 . + 0 Rv1143 mcr Required for bile acid synthesis and for catabolism of branched-chain fatty acids Probable alpha-methylacyl-CoA racemase Mcr (2-methylacyl-CoA racemase) (2-arylpropionyl-CoA epimerase ) Rv1143, (MTCI65.10), len: 360 aa. Probable mcr, alpha-methylacyl-CoA racemase. Strong similarity to other alpha-methylacyl-CoA racemases and also some similarity to L-carnitine dehydratase e.g. U89905|g1552373 methylacyl-CoA racemase alpha from Norway rat (361 aa), FASTA scores: opt: 1035, E():0, (47.2% identity in 339 aa overlap). Equivalent to (but longer than) Z94723|MLCB33_13 Mycobacterium leprae (253 aa) (85.3% identity in 245 aa overlap). Also similar to Mycobacterium tuberculosis putative racemases Rv0855, Rv1866, Rv3272. O06543 No lipid metabolism 1X74,2GCE,2GCI,2GD0,2GD2,2GD6 O06543 O06543 GO:0008152,GO:0008111 5.1.99.4 O06543 ML0969 MSMEG_5184 Mb1175 NC_000962.3 Mycobrowser_v4 CDS 1271156 1271908 . + 0 Rv1144 Rv1144 Function unknown; supposedly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv1144, (MTCI65.11), len: 250 aa. Probable short-chain dehydrogenase/reductase, highly similar to various dehydrogenases e.g. NP_104056.1|NC_002678 3-hydroxyacyl-CoA dehydrogenase type II from Mesorhizobium loti (253 aa); NP_251244.1|NC_002516 probable short-chain dehydrogenase from Pseudomonas aeruginosa (255 aa); AAK15008.1|AF233685_1|AF233685 short chain L-3-hydroxyacyl-CoA dehydrogenase from Mus musculus (261 aa); HSU73514|g1778354|XH98G2 human short-chain alcohol dehydrogenase from Homo sapiens (261 aa), FASTA scores: opt: 875, E(): 0, (60.1% identity in 253 aa overlap); etc. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. P9WGQ7 No intermediary metabolism and respiration O06544 P9WGQ7 GO:0055114,GO:0016491 1.-.-.- P9WGQ7 ML0970 MMAR_4307 MSMEG_5183 Mb1176 NC_000962.3 Mycobrowser_v4 CDS 1272423 1273334 . + 0 Rv1145 mmpL13a Unknown. Thought to be involved in fatty acid transport. Probable conserved transmembrane transport protein MmpL13a Rv1145, (MTCI65.12), len: 303 aa. Probable mmpL13a, conserved transmembrane transport protein (see citation below), member of RND superfamily, showing some similarity to putative Mycobacterial and Streptomyces membrane proteins e.g. MTCY987|g1781238 from Mycobacterium tuberculosis (962 aa), FASTA scores: opt: 213, E(): 1.9e-06, (28.0% identity in 296 aa overlap); etc. Strong similarity to U92075|MMU92075_5 hypothetical protein from Mycobacterium marinum (256 aa), FASTA scores: opt: 957, E(): 0, (57.6% identity in 257 aa overlap). Should continue as mmpL13B|Rv1146, but frameshift required. Sequence has been checked and is identical in M. tuberculosis strain CDC1551, and Mycobacterium bovis strain AF2122/97. Belongs to the MmpL family. O06545 No cell wall and cell processes O06545 O06545 GO:0016021 O06545 ML0971 Mb1177 NC_000962.3 Mycobrowser_v4 CDS 1278904 1279617 . - 0 Rv1151c Rv1151c Involved in transcriptional mechanism Transcriptional regulatory protein Rv1151c, (MTCI65.18c), len: 237 aa. Transcriptional regulatory protein, similar to others AE000776|AE000776_10 Aquifex aeolicus (239 aa), FASTA scores: opt: 725, E(): 0, (46.4% identity in 237 aa overlap); ECAE0002125|g1787358 Escherichia coli (279 aa), FASTA scores: opt: 464, E(): 1.3e-23, (36.7% identity in 240 aa overlap). P9WGG3 No regulatory proteins P66813 P9WGG3 GO:0006342,GO:0005737,GO:0016811,GO:0006476,GO:0008270,GO:0070403 3.5.1.- P9WGG3 ML1511 MMAR_4302 MSMEG_5175 Mb1182c NC_000962.3 Mycobrowser_v4 CDS 1279655 1280020 . + 0 Rv1152 Rv1152 Involved in transcriptional mechanism Probable transcriptional regulatory protein Rv1152, (MTCI65.19), len: 121 aa (Start uncertain). Probable transcriptional regulatory protein, some similarity to others e.g. YHCF_BACSU hypothetical transcriptional regulator (121 aa), FASTA scores: opt: 187, E(): 1.9e-06, (34.9% identity in 106 aa overlap). Helix turn helix motif from aa 42-63 (+3.10 SD). O06550 No regulatory proteins O06550 O06550 GO:0006355,GO:0006350,GO:0003700,GO:0005622 O06550 ML1510,ML1510c MMAR_4301 MSMEG_5174 Mb1183 NC_000962.3 Mycobrowser_v4 CDS 1332980 1333858 . + 0 Rv1190 Rv1190 Function unknown Conserved hypothetical protein Rv1190, (MTCI364.02), len: 292 aa. Conserved hypothetical protein, similar to Rv1833c|Y0DA_MYCTU|Q50600 hypothetical 32.2 kDa protein cy1a11.10 (286 aa), fasta scores: opt: 331, E(): 1.4e-15, (29.0% identity in 272 aa overlap), also YU14_MYCTU|Q50670 putative haloalkane dehalogenase (300 aa), FASTA scores: opt: 239, E(): 2.2e-09, (29.9% identity in 298 aa overlap). O86348 No conserved hypotheticals O86348 O86348 GO:0016787 O86348 Mb1222 NC_000962.3 Mycobrowser_v4 CDS 1278269 1278820 . + 0 Rv1150 Rv1150 Possibly required for the transposition of an insertion element Possible transposase (fragment) Rv1150, (MTCI65.17), len: 183 aa. Possible fragment of transposase (pseudogene). Identical to C-terminal part of S21394 transposase of putative Mycobacterium tuberculosis is element (308 aa), FASTA scores: opt: 959, E(): 0, (99.3% identity in 145 aa overlap). The transposase described here may be made by a -1 frame shifting mechanism during translation that fuses Rv1149|MTCI65.16 and Rv1150|MTCI65.17. No evidence found to account for discrepancy with previously published sequence. Second copy is Rv1041c|MTCY10G2.08. Yes insertion seqs and phages Mb1181 NC_000962.3 Mycobrowser_v4 ncRNA 1283693 1283815 . + 0 MTB000072 mcr10 Unknown Putative small regulatory RNA mcr10, putative small regulatory RNA (See DiChiara et al., 2010). 5'-end mapped by 5'RLM-RACE in M. bovis BGC Pasteur, 3'-end not mapped, ~118 nt band detected by Northern blot. No stable RNAs NC_000962.3 Mycobrowser_v4 CDS 1274900 1275550 . + 0 Rv1147 Rv1147 Function unknown Conserved protein Rv1147, (MTCI65.14), len: 216 aa. Conserved protein, similar to many conserved hypothetical proteins, and some similarity to several methyltransferases e.g. Q05197|PMTA_RHOSH phosphatidylethanolamine N-methyltransferase from R. sphaeroides (203 aa), FASTA scores: opt: 156, E(): 0.00073, (27.6% identity in 156 aa overlap). O06547 No conserved hypotheticals O06547 O06547 GO:0008168,GO:0008152 O06547 ML0973 MMAR_4303 MSMEG_5176 Mb1178 NC_000962.3 Mycobrowser_v4 CDS 1276300 1277748 . - 0 Rv1148c Rv1148c Unknown Conserved hypothetical protein Rv1148c, (MTCI65.15c), len: 482 aa. Conserved hypothetical ORF in REP13E12 degenerate repeat, nearly identical to other hypothetical Mycobacterium tuberculosis proteins in REP13E12 repeats, although similarity extends upstream past proposed f-Met start. Very similar to other REP13E12 proteins e.g. Rv1945, Rv3467, Rv0094c, Rv1128c etc. P9WM55 No insertion seqs and phages P0A5D9 P9WM55 P9WM55 MSMEG_2663,MSMEG_6573 Mb1179c NC_000962.3 Mycobrowser_v4 CDS 1277893 1278300 . + 0 Rv1149 Rv1149 Possibly required for the transposition of an insertion element Possible transposase Rv1149, (MTCI65.16), len: 135 aa. Possible transposase. Identical to 117 aa N-terminal region of S21394|X65618 transposase of Mycobacterium tuberculosis (308 aa), FASTA scores: opt: 823, E(): 0, (99.1% identity in 117 aa overlap). Second copy is Rv1042c|MTCY10G2.07. L0T897 No insertion seqs and phages L0T897 Mb1180 NC_000962.3 Mycobrowser_v4 CDS 1281429 1281872 . + 0 Rv1155 Rv1155 May be involved in biosynthesis of pyridoxine (vitamin B6) and pyridoxal phosphate. Oxidize PNP and PMP into pyridoxal 5'-phosphate (PLP) [catalytic activity: pyridoxamine 5'-phosphate + H2O + O2 = pyridoxal 5'-phosphate + NH3 + H2O2] Possible pyridoxamine 5'-phosphate oxidase (PNP/PMP oxidase) (pyridoxinephosphate oxidase) (PNPOX) (pyridoxine 5'-phosphate oxidase) Rv1155, (MTCI65.22), len: 147 aa. Possible pyridoxine 5'-phosphate oxidase (PNPOx) (See Biswal et al., 2005; Canaan et al., 2005). Similar to hypothetical proteins e.g. AL079356|SC6G9.20 Streptomyces coelicolor (144 aa), FASTA scores: opt: 478, E(): 2.8e-26, (55.7% identity in 140 aa overlap); and Mycobacterium tuberculosis proteins Rv1875, Rv0121c, Rv2074. O06553 No intermediary metabolism and respiration 1W9A,1XXO,1Y30,2AQ6 O06553 O06553 GO:0010181 O06553 ML1508,ML1508c MMAR_4298 MSMEG_5170 Mb1186 NC_000962.3 Mycobrowser_v4 CDS 1283056 1284171 . - 0 Rv1157c Rv1157c Function unknown Conserved ala-, pro-rich protein Rv1157c, (MTCI65.24c), len: 371 aa. Conserved Ala-, Pro-rich protein, similar to other proline rich proteins and extensins e.g. GBU04267|g451543 sea-island cotton proline-rich protein of cotton fiber (214 aa), FASTA scores: opt: 305, E(): 3.9e-05, (35.7% identity in 182 aa overlap). Has hydrophobic stretch at N-terminus suggestive of secretion signal. First start taken. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06555 No conserved hypotheticals O06555 GO:0005199 ML1506 MMAR_4294 MSMEG_5153 Mb1188c NC_000962.3 Mycobrowser_v4 CDS 1701295 1702593 . + 0 Rv1510 Rv1510 Unknown Conserved probable membrane protein Rv1510, (MTCY277.32), len: 432 aa. Probable membrane protein. Highly similar to Rv3630|MTCY15C10.22 (431 aa), FASTA scores: E(): 0, (70.8% identity in 424 aa overlap). P9WLW1 No cell wall and cell processes P9WLW1 GO:0005886,GO:0016021 MSMEG_6140 NC_000962.3 Mycobrowser_v4 CDS 1286284 1286568 . - 0 Rv1159A Rv1159A Unknown Unknown protein Rv1159A, len: 94 aa. Unknown protein. P0A5S2 No conserved hypotheticals P0A5S2 P0A5S2 GO:0006729,GO:0008124 4.2.1.96 ML1503A MMAR_4291 MSMEG_5150 Mb1191c NC_000962.3 Mycobrowser_v4 CDS 1286595 1287020 . + 0 Rv1160 mutT2 Involved in the go system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite DA and DC residues of template DNA with almost equal efficiency thus leading to A.T to G.C transversions. MUTT2 specifically degrades 8-oxo-dGTP to the monophosphate. Probable mutator protein MutT2 (7,8-dihydro-8-oxoguanine-triphosphatase) (8-oxo-dGTPase) Rv1160, (MTCI65.27), len: 141 aa. Probable mutT2, mutator protein or homolog (see citation below). More similar to D908197|g1742860 MutT homolog from Escherichia coli (135 aa), FASTA scores: opt: 226, E():1.1e-08, (39.7% identity in 116 aa overlap); than to MUTT_ECOLI|P08337 mutator mutt protein from Escherichia coli (129 aa), FASTA scores: opt: 180, E(): 1.2e-05, (27.1% identity in 129 aa overlap). Contains PS00893 mutT domain signature. P9WIY1 No information pathways O06558 P9WIY1 GO:0006260,GO:0016787,GO:0046872,GO:0006281 3.6.1.- P9WIY1 ML1503,ML1503c MMAR_4290 MSMEG_5148 Mb1192 NC_000962.3 Mycobrowser_v4 CDS 1287328 1291026 . + 0 Rv1161 narG Nitrate reduction [catalytic activity: nitrite + acceptor = nitrate + reduced acceptor]. Respiratory nitrate reductase (alpha chain) NarG Rv1161, (MTCI65.28), len: 1232 aa. narG, respiratory nitrate reductase alpha chain. Similar to others e.g. NARG_BACSU nitratereductase alpha chain from Bacillus subtilis (1228 aa), FASTA scores: opt: 4218, E(): 0, (50.3% identity in 1229 aa overlap); etc. Also highly similar to N-terminal part of Rv1736c|MTCY04C12.21c|NARX probable nitrate reductase from Mycobacterium tuberculosis (85.1% identity in 281 aa overlap). Contains prokaryotic molybdopterin oxidoreductase signatures 1 and 2 (PS00551, PS00490). Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. P9WJQ3 No intermediary metabolism and respiration O06559 P9WJQ3 GO:0009055,GO:0022900,GO:0030151,GO:0042128,GO:0008940,GO:0009325,GO:0005886,GO:0006810,GO:0051539 1.7.99.4 P9WJQ3 ML1502,ML1502c MSMEG_5140 Mb1193 NC_000962.3 Mycobrowser_v4 CDS 1292798 1293403 . + 0 Rv1163 narJ Nitrate reduction [catalytic activity: nitrite + acceptor = nitrate + reduced acceptor]. Probable respiratory nitrate reductase (delta chain) NarJ Rv1163, (MTCI65.30), len: 201 aa. Probable narJ, respiratory nitrate reductase delta chain. Similar to others e.g. P42178|NARJ_BACSU nitrate reductase delta chain from Bacillus subtilis (184 aa), FASTA scores: opt: 254, E(): 1.9e-10, (31.8% identity in 179 aa overlap); etc. Strong similarity to region from aa 260 - 410 of Rv1736c|MTCY04C12.21c|NARX probable nitrate reductase from Mycobacterium tuberculosis (64.8% identity in 159 aa overlap). O06561 No intermediary metabolism and respiration O06561 O06561 GO:0009325,GO:0055114,GO:0008940 1.7.99.4 O06561 ML1500,ML1500c MSMEG_5138 Mb1195 NC_000962.3 Mycobrowser_v4 CDS 1353157 1353525 . + 0 Rv1209 Rv1209 Function unknown Conserved protein Rv1209, (MTCI364.21), len: 122 aa. Conserved protein, containing a hydrophobic N-terminus. Similar to Q49956|U1756M hypothetical protein from Mycobacterium leprae (114 aa), FASTA scores: opt: 524, E(): 8.9e-29, (78.6% identity in 112 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). O05310 No conserved hypotheticals O05310 ML1065 MMAR_4229 MSMEG_5083 Mb1241 NC_000962.3 Mycobrowser_v4 CDS 1676941 1677375 . + 0 Rv1487 Rv1487 Unknown Conserved membrane protein Rv1487, (MTCY277.08), len: 144 aa. Conserved membrane protein. Highly similar to O07404|AF002133 MAV145 from Mycobacterium avium (145 aa), FASTA scores: opt: 667, E(): 0, (72.5% identity in 142 aa overlap). Also similar to AL079332|SCI5.05 hypothetical protein from Streptomyces coelicolor (143 aa), FASTA scores: opt: 344, E(): 1.3e-15, (44.8% identity in 134 aa overlap). P71767 No cell wall and cell processes P71767 P71767 P71767 ML1803,ML1803c MMAR_2293 MSMEG_3154 Mb1523 NC_000962.3 Mycobrowser_v4 CDS 1296152 1298059 . + 0 Rv1166 lpqW Unknown Probable conserved lipoprotein LpqW Rv1166, (MTV005.02), len: 635 aa. Probable lpqW, conserved lipoprotein, almost identical in part to G2384665|AF009358 Mycobacterium tuberculosis gene fragment ORFA2-898 (fragment) (59 aa) (93.9% identity in 49 aa overlap) (see * below). Also similar to Rv1280c and Rv2585c. Contains possible N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. [* Note: Unpublished. Identification of Mycobacterium tuberculosis peptides that stimulate immune human peripheral blood monocytes. Nano F.E., Doran J.L., Treit J.D., Moran A.J.] P9WGU7 No cell wall and cell processes O50422 P9WGU7 GO:0008654,GO:0006810,GO:0005215,GO:0009405 P9WGU7 ML1497,ML1497c MMAR_4288 MSMEG_5130 Mb1198 NC_000962.3 Mycobrowser_v4 CDS 1298087 1298692 . - 0 Rv1167c Rv1167c Involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv1167c, (MTV005.03c), len: 201 aa. Probable transcriptional regulator, similar to several e.g. D1022772|D85417 hemR from Propionibacterium freudenreichii (243 aa), FASTA scores: opt: 268, E(): 5.4e-16, (35.9% identity in 198 aa overlap) and AL022268|SC4H2.32 Streptomyces coelicolor (111 aa), FASTA scores: opt: 274, E(): 5e-11, (55.1% identity in 89 aa overlap). O50423 No regulatory proteins O50423 O50423 GO:0006350,GO:0003700,GO:0006355 O50423 ML1496 MMAR_4284 Mb1199c NC_000962.3 Mycobrowser_v4 CDS 1298764 1299804 . - 0 Rv1168c PPE17 Function unknown PPE family protein PPE17 Rv1168c, (MTV005.04c), len: 346 aa. PPE17, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, similar to many e.g. E332789|Z98268|MTCI125.27C (385 aa), FASTA scores: opt: 504, E(): 0, (36.6% identity in 388 aa overlap). P9WI27 No PE/PPE Q7D8Q2 P9WI27 P9WI27 Mb1200c,Mb1201c NC_000962.3 Mycobrowser_v4 CDS 1299822 1300124 . - 0 Rv1169c lipX Function unknown PE family protein. Possible lipase LipX. Rv1169c, (MTV005.05c), len: 100 aa. Possible lipX, lipase. Member of the Mycobacterium tuberculosis PE family of proteins (see Brennan & Delogu 2002), e.g. O05297|Z93777|MTCI364.07 (99 aa), FASTA scores: opt: 209, E(): 1.6e-15, (37.4% identity in 99 aa overlap). Also simlar to the N-terminus of P77909|U76006 esterase/lipase from Mycobacterium tuberculosis (437 aa), FASTA scores: opt: 193, E(): 4.4e-14, (37.2% identity in 94 aa overlap). Contains a helix-turn-helix motif from aa 88-109 (+2.76 SD). Predicted possible vaccine candidate (See Zvi et al., 2008). Q79FR5 No PE/PPE Q79FR5 Q79FR5 Q79FR5 Mb1202c NC_000962.3 Mycobrowser_v4 CDS 1357759 1359444 . - 0 Rv1215c Rv1215c Function unknown Conserved protein Rv1215c, (MTCI364.27c), len: 561 aa. Conserved protein, low similarity to Rv1835c|Y0D8_MYCTU|Q50598 hypothetical 69.9 kDa protein cy1a11.08 (628 aa), FASTA scores: opt: 257, E(): 1.3e-09, (34.1% identity in 185 aa overlap). O05316 No conserved hypotheticals O05316 O05316 GO:0008152,GO:0008239 O05316 MMAR_1269 MSMEG_5283 Mb1247c NC_000962.3 Mycobrowser_v4 CDS 1301755 1302681 . - 0 Rv1172c PE12 Function unknown PE family protein PE12 Rv1172c, (MTV005.08c), len: 308 aa. PE12, Member of the Mycobacterium tuberculosis PE family of proteins (see Brennan & Delogu 2002), e.g. P71748|Z81368|MTCY253.25C (361 aa), FASTA scores: opt: 483, E(): 7.8e-22, (46.4% identity in 192 aa overlap). L7N693 No PE/PPE Q7D8P8 L7N693 L7N693 ML1493 MMAR_4280 Mb1205c NC_000962.3 Mycobrowser_v4 CDS 1305669 1306001 . - 0 Rv1174c TB8.4 Unknown function (secreted protein) Low molecular weight T-cell antigen TB8.4 Rv1174c, (MTV005.10c), len: 110 aa. TB8.4, low molecular weight T-cell antigen (see citations below), hypothetical unknown secreted protein. Predicted to be an outer membrane protein (See Song et al., 2008). Predicted possible vaccine candidate (See Zvi et al., 2008). O50430 No cell wall and cell processes O50430 ML1491 MMAR_4277 MSMEG_4804,MSMEG_5934 Mb1207c NC_000962.3 Mycobrowser_v4 CDS 1306202 1308226 . - 0 Rv1175c fadH Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-2- enoyl-CoA [catalytic activity: trans-2,3-didehydroacyl-CoA + NADP(+) = trans,trans-2,3,4,5-tetradehydroacyl-CoA + NADPH]. Probable NADPH dependent 2,4-dienoyl-CoA reductase FadH (2,4-dienoyl coenzyme A reductase) (4-enoyl-CoA reductase) Rv1175c, (MTV005.11c), len: 674 aa. Probable fadH, NADPH-dependent 2,4-dienoyl-CoA reductase, highly similar to others e.g. NP_251782.1|NC_002516 2,4-dienoyl-CoA reductase FadH1 from Pseudomonas aeruginosa (679 aa); CAC01564.1|AL391039 2,4-dienoyl-CoA reductase [NADPH] from Streptomyces coelicolor (671 aa); P42593|FADH_ECOLI 2,4-dienoyl-CoA reductase from Escherichia coli (671 aa), FASTA scores: opt: 2344, E(): 0, (53.1% identity in 671 aa overlap); etc. Also similar to Rv3359|MTV004.16 putative oxidoreductase from Mycobacterium tuberculosis (396 aa). O50431 No lipid metabolism O50431 O50431 GO:0050660,GO:0010181,GO:0055114,GO:0008670 1.3.1.34 O50431 ML1490 MMAR_4276 MSMEG_5124 Mb1208c NC_000962.3 Mycobrowser_v4 CDS 1309005 1309331 . + 0 Rv1177 fdxC Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Probable ferredoxin FdxC Rv1177, (MTV005.13), len: 108 aa. Probable fdxC, ferredoxin, equivalent to NP_302047.1|NC_002677 ferredoxin from Mycobacterium leprae (108 aa); P00215|FER_MYCSM ferredoxin from Mycobacterium smegmatis (106 aa), FASTA scores: opt: 705, E(): 0, (87.7% identity in 106 aa overlap). Also highly similar to many e.g. JH0239 ferredoxin precursor from Saccharopolyspora erythraea (105 aa); P24496|FER_SACER ferredoxin from Saccharopolyspora erythraea (106 aa); etc. Contains PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature. Belongs to the bacterial type ferredoxin family. Cofactor: binds 1 4FE-4S cluster and a 3FE-4S cluster (by similarity). O50433 No intermediary metabolism and respiration O50433 O50433 GO:0009055,GO:0046872,GO:0055114,GO:0016491,GO:0051539 1.-.-.- O50433 ML1489,ML1489c MMAR_4274 MSMEG_5122 Mb1210 NC_000962.3 Mycobrowser_v4 CDS 1313725 1315191 . + 0 Rv1180 pks3 Potentially involved in some intermediate steps for the synthesis of a polyketide molecule which may be involved in secondary metabolism. Supposedly involved in stationary-phase survival. Probable polyketide beta-ketoacyl synthase Pks3 Rv1180, (MTV005.16), len: 488 aa. Probable polyketide beta-ketoacyl synthase, equivalent to a predicted homologous protein from Mycobacterium smegmatis (see citation below), and similar to the N-terminus of many polyketide synthases e.g. MCAS_MYCBO|Q02251 mycocerosic acid synthase from Mycobacterium bovis (2110 aa), FASTA scores: opt: 2115, E(): 0, (66.5% identity in 472 aa overlap). Also similar to, and same length as P96284|Z83858|MTCY24G1.02 M. tuberculosis (496 aa), FASTA scores: opt: 1424, E(): 0, (50.9% identity in 444 aa overlap). Contains possible signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site, also PS00606 Beta-ketoacyl synthases active site. Belongs to the beta-ketoacyl-ACP synthases family. Alternative nucleotide at position 1315191 (a->C; Stop489Y) has been observed. Rv1180/Rv1181 fusion has been called msl3. O50436 No lipid metabolism O50436 O50436 GO:0009058,GO:0008415 2.3.1.- A0A089QRB9 ML1229 Mb1213 NC_000962.3 Mycobrowser_v4 CDS 1315234 1319982 . + 0 Rv1181 pks4 Potentially involved in some intermediate steps for the synthesis of a polyketide molecule which may be involved in secondary metabolism. Probable polyketide beta-ketoacyl synthase Pks4 Rv1181, (MTV005.17), len: 1582 aa. Probable pks4, polyketide synthase, similar to many e.g. MCAS_MYCBO|Q02251 mycocerosic acid synthase from Mycobacterium bovis (2110 aa), FASTA scores: opt: 3518, E(): 0, (59.7% identity in 1614 aa overlap). Note that this similarity extends upstream of the first initiation codon into the upstream MTV005.16; the stop codon at the end of MTV005.16 is present in at least 4 independent clones (BAC, cosmid and pUC) from the genome (however an alternative nucleotide at position 1315191 (a->C; Stop489Y) has also been observed). The two CDS's may represent separate modules of the polyketide synthase. Rv1180/Rv1181 fusion has been called msl3. O50437 No lipid metabolism O50437 O50437 GO:0008415,GO:0009058,GO:0048037,GO:0055114,GO:0016491,GO:0008270,GO:0000036 2.3.1.- A0A089QRB9 Mb1213 NC_000962.3 Mycobrowser_v4 CDS 1320035 1321453 . + 0 Rv1182 papA3 Function unknown; thought to be involved in lipid metabolism. Probable conserved polyketide synthase associated protein PapA3 Rv1182, (MTV005.18), len: 472 aa. Probable papA3, conserved polyketide synthase (PKS) associated protein, similar to other Mycobacterial hypothetical proteins e.g. Q49618|U00010 B1170_C1_180 from Mycobacterium leprae (471 aa), FASTA scores: opt: 2526, E(): 0, (75.6% identity in 471 aa overlap). Similar to other Mycobacterium tuberculosis hypothetical papA proteins; Rv3824c, Rv3820c, Rv1528c. P9WIK5 No lipid metabolism O50438 P9WIK5 P9WIK5 ML1230 MSMEG_0409 Mb1214 NC_000962.3 Mycobrowser_v4 CDS 1332092 1332964 . + 0 Rv1189 sigI The sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released Possible alternative RNA polymerase sigma factor SigI Rv1189, (MTV005.25-MTCI364.01), len: 290 aa. Possible sigI, alternative RNA polymerase sigma factor (see Gomez et al., 1997; Chen et al., 2000), similar to several e.g. O05767|U87307 extracytoplasmic function alternative sigma factor (sigE) from Mycobacterium smegmatis (204 aa), FASTA scores: opt: 239, E(): 1.3e-09, (32.9% identity in 167 aa overlap). P9WGH3 No information pathways O50445 P9WGH3 GO:0016987,GO:0003700,GO:0006352,GO:0006355 P9WGH3 Mb1221 NC_000962.3 Mycobrowser_v4 CDS 1340659 1340955 . + 0 Rv1197 esxK Function unknown ESAT-6 like protein EsxK (ESAT-6 like protein 3) Rv1197, (MT1235, MTCI364.09), len: 98 aa. EsxK, ESAT-6 like protein (see citation below). Member of M. tuberculosis hypothetical QILSS protein family with Rv1038c, etc. Almost identical to MTCY98.023c (98 aa) (99.0% identity in 98 aa overlap) and MTCY10G2.11 (98 aa), FASTA scores: opt: 643, E(): 0, (99.0% identity in 98 aa overlap); highly similar to Q49945|U1756C from Mycobacterium leprae (100 aa), FASTA scores: opt: 377, E(): 8e-21, (58.3% identity in 96 aa overlap). Belongs to the ESAT6 family. P9WNJ7 No cell wall and cell processes P9WNJ7 P9WNJ7 ML1055,ML1181c MMAR_2674,MMAR_3654,MMAR_3663,MMAR_4453,MMAR_5120 Mb1229 NC_000962.3 Mycobrowser_v4 CDS 1324532 1325611 . - 0 Rv1184c Rv1184c Function unknown Possible exported protein Rv1184c, (MTV005.20c), len: 359 aa. Possible exported protein with potential N-terminal signal sequence. Similar to several Mycobacterial hypothetical proteins e.g. Q49633|U00010 Protein B1170_F3_112 from M. leprae (391 aa), FASTA scores: opt: 1422, E(): 0, (62.7% identity in 338 aa overlap). Also similar to Rv3822, Rv3539, Rv1430, Rv0151c, etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O50440 No cell wall and cell processes O50440 O50440 ML1232,ML1232c MSMEG_0412 Mb1216c NC_000962.3 Mycobrowser_v4 CDS 1325776 1327512 . - 0 Rv1185c fadD21 Function unknown, but supposedly involved in lipid degradation. Probable fatty-acid-AMP ligase FadD21 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) Rv1185c, (MTV005.21c), len: 578 aa. Probable fadD21, fatty-acid-AMP synthetase, highly similar to several from Mycobacteria e.g. NP_301895.1|NC_002677 possible acyl-CoA synthase from Mycobacterium leprae (579 aa); P71495|U75685 acyl-CoA synthase from Mycobacterium bovis (582 aa), FASTA scores: opt: 2388, E(): 0, (61.8% identity in 579 aa overlap); etc. Seems to belong to the ATP-dependent AMP-binding enzyme family. Nucleotide position 1327402 in the genome sequence has been corrected, T:C resulting in E37E. P9WQ49 No lipid metabolism P63523 P9WQ49 GO:0016874,GO:0006631 6.2.1.- P9WQ49 ML1234,ML1234c MSMEG_4731 Mb1217c NC_000962.3 Mycobrowser_v4 CDS 1327689 1329305 . - 0 Rv1186c Rv1186c Function unknown Conserved protein Rv1186c, (MTV005.22c), len: 538 aa. Conserved protein, similar to AL117385|SC5G9.24 hypothetical protein from Streptomyces coelicolor (555 aa), FASTA scores: opt: 485, E(): 2.3e-23, (32.6% identity in 568 aa overlap). Contains helix turn helix motif from aa 488-509 (+2.81 SD). O50442 No conserved hypotheticals O50442 O50442 MMAR_4254 MSMEG_5120 Mb1218c NC_000962.3 Mycobrowser_v4 CDS 1339349 1340524 . + 0 Rv1196 PPE18 Function unknown PPE family protein PPE18 Rv1196, (MTCI364.08), len: 391 aa. PPE18 (alternate gene name: mtb39a). Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, highly similar to others e.g. Y07P_MYCTU|Q11031 hypothetical 40.0 kDa protein cy02b10.25c (396 aa), FASTA scores: opt: 2124, E(): 0, (85.1% identity in 397 aa overlap). Note that expression of Rv1196 was demonstrated in lysates by immunodetection (see Dillon et al., 1999). L7N675 No PE/PPE Q7D8M9 L7N675 L7N675 ML1054,ML1182c MMAR_4240 Mb1228 NC_000962.3 Mycobrowser_v4 CDS 1346936 1348624 . - 0 Rv1204c Rv1204c Function unknown Conserved hypothetical protein Rv1204c, (MTCI364.16c), len: 562 aa. Conserved hypothetical protein, some similarity to Q55103 CHO-ORF2 from streptomyces SP. (642 aa), FASTA scores: opt: 215, E(): 3.6e-06, (26.4% identity in 576 aa overlap). Contains PS00017 ATP/GTP-binding site motif A. O05305 No conserved hypotheticals O05305 GO:0017111,GO:0005524 O05305 ML1060,ML1060c MMAR_4234 MSMEG_5088 Mb1236c NC_000962.3 Mycobrowser_v4 CDS 1359472 1360146 . - 0 Rv1216c Rv1216c Function unknown Probable conserved integral membrane protein Rv1216c, (MTCI364.28c), len: 224 aa. Probable conserved integral membrane protein, C-terminal region similar to Q49963|U1756P from Mycobacterium leprae (134 aa), FASTA scores: opt: 311, E(): 3.3e-15, (52.2% identity in 113 aa overlap). O05317 No cell wall and cell processes O05317 O05317 GO:0016021,GO:0004671,GO:0006481 O05317 ML1071,ML1071c MMAR_4222 MSMEG_5077 Mb1248c NC_000962.3 Mycobrowser_v4 ncRNA 1340578 1340625 . + 0 MTB000075 ncrMT1234 Unknown Fragment of putative small regulatory RNA ncrMT1234, fragment of putative small regulatory RNA (See Pelly et al., 2012), cloned from M. tuberculosis CDC1551; supported by RNA-seq in H37Rv (unpublished data). No stable RNAs NC_000962.3 Mycobrowser_v4 CDS 1331021 1332010 . + 0 Rv1188 Rv1188 Oxidizes proline to glutamate for use as a carbon and nitrogen source [catalytic activity: L-proline + acceptor + H2O = (S)-1-pyrroline-5-carboxylate + reduced acceptor] Probable proline dehydrogenase Rv1188, (MTV005.24), len: 329 aa. Possible putA, proline dehydrogenase, similar to part of Q52711|X78346 proline dehydrogenase from Rhodobacter capsulatus (1127 aa), FASTA scores: opt: 194, E(): 1.5e-07, (31.2% identity in 349 aa overlap). Also similar to two Bacillus subtilis proline dehydrohenases E1184363|Z99120 (302 aa), FASTA scores: opt: 509, E(): 0, (37.1% identity in 313 aa overlap); and E1182272|Z99105 (303 aa), FASTA scores: opt: 513, E(): 0, (32.5% identity in 311 aa overlap). Highly similar to AL035569|SC8D9.31 Streptomyces coelicolor (308 aa), FASTA scores: opt: 984, E(): 0, (50.0% identity in 312 aa overlap). Nucleotide position 1331696 in the genome sequence has been corrected, A:C resulting in R226R. O50444 No intermediary metabolism and respiration O50444 O50444 GO:0055114,GO:0006562,GO:0004657,GO:0006537 1.5.99.8 O50444 MMAR_4252 MSMEG_5117 Mb1220 NC_000962.3 Mycobrowser_v4 CDS 1333931 1334845 . + 0 Rv1191 Rv1191 Function unknown Conserved protein Rv1191, (MTCI364.03), len: 304 aa. Conserved protein, similar to Q54528 RDMC from Streptomyces purpurascens (298 aa), FASTA scores: opt: 196, E(): 1.5e-05, (27.5% identity in 269 aa overlap); Rv0134|MTCI5.08 (300 aa), FASTA scores: opt: 197, E(): 6.6e-06, (26.4% identity in 299 aa overlap), some similarity to PIP_NEIGO|P42786 proline iminopeptidase (310 aa), FASTA scores: opt: 196, E(): 1.3e-05, (32.2% identity in 152 aa overlap). Contains PS00044 Bacterial regulatory proteins, lysR family signature. O05293 No conserved hypotheticals O05293 O05293 O05293 ML1050 MSMEG_3508 Mb1223 NC_000962.3 Mycobrowser_v4 CDS 1334927 1335754 . + 0 Rv1192 Rv1192 Unknown Unknown protein Rv1192, (MTCI364.04), len: 275 aa. Unknown protein, contains PS00120 lipases, serine active site. O05294 No conserved hypotheticals O05294 O05294 O05294 MMAR_4244 Mb1224 NC_000962.3 Mycobrowser_v4 CDS 1337248 1338513 . - 0 Rv1194c Rv1194c Function unknown Conserved protein Rv1194c, (MTCI364.06c), len: 421 aa. Conserved protein, highly similar to Q50018 possible transcriptional activator from Mycobacterium leprae (517 aa), FASTA scores: opt: 1960, E(): 0, (69.8% identity in 421 aa overlap). Also similar to Mycobacterium tuberculosis Rv2370c|MTCY27.10, (62.0% identity in 421 aa overlap) and Rv1453|MTCY493.01c. O05296 No conserved hypotheticals O05296 O05296 ML1052,ML1052c MMAR_4242 Mb1226c NC_000962.3 Mycobrowser_v4 CDS 1339003 1339302 . + 0 Rv1195 PE13 Function unknown PE family protein PE13 Rv1195, (MTCI364.07), len: 99 aa. PE13, Member of Mycobacterium tuberculosis PE family (see Brennan & Delogu 2002), e.g. Y0DP_MYCTU|Q50615 hypothetical glycine-rich 40.8 kd protein (498 aa), FASTA scores: opt: 307, E(): 1.4e-12, (56.3% identity in 96 aa overlap), similar to MTCY21C12.10c (99 aa), FASTA scores: opt:295, E(): 1.9e-11, (51.5% identity in 97 aa overlap). Q79FR3 No PE/PPE Q79FR3 Q79FR3 Q79FR3 ML1053,ML1183c MMAR_4241 Mb1227 NC_000962.3 Mycobrowser_v4 CDS 1342942 1344219 . + 0 Rv1200 Rv1200 Unknown; probably involved in transport accross the membrane (probably sugar transport). Probable conserved integral membrane transport protein Rv1200, (MTCI364.12), len: 425 aa. Probable conserved integral membrane transport protein, possibly member of major facilitator superfamily (MFS), similar to others e.g. YHJE_ECOLI|P37643 hypothetical metabolite transport protein from Escherichia coli (440 aa), FASTA scores: opt: 1047, E(): 0, (39.1% identity in 427 aa overlap); etc. Contains PS00217 Sugar transport proteins signature 2. The transcription of this CDS seems to be activated in macrophages (see citation below). O05301 No cell wall and cell processes O05301 O05301 GO:0016021,GO:0055085,GO:0005215,GO:0008643 MMAR_4238 Mb1232 NC_000962.3 Mycobrowser_v4 CDS 1344216 1345169 . - 0 Rv1201c dapD Involved in biosynthesis of diaminopimelate and lysine from aspartate semialdehyde (at the third step) [catalytic activity: tetrahydrodipicolinate + succinyl-CoA + H2O = N-succinyl-2-amino-6-ketopimelate + coenzyme A] Tetrahydrodipicolinate N-succinyltransferase DapD Rv1201c, (MTCI364.13c), len: 317 aa. dapD, tetrahydrodipicolinate N-succinyltransferase. Highly similar to Q49948|U1756F Mycobacterium leprae (317 aa), FASTA scores: opt: 1776, E(): 0, (84.9% identity in 317 aa overlap), also Q46064 ORF3 protein from corynebacterium glutamicum (316 aa), FASTA scores: opt: 864, E(): 0, (44.1% identity in 311 aa overlap). P9WP21 No intermediary metabolism and respiration 3FSX,3FSY P9WP21 GO:0008666 2.3.1.117 P9WP21 ML1058,ML1058c MMAR_4236 MSMEG_5104 Mb1233c NC_000962.3 Mycobrowser_v4 CDS 1346321 1346905 . - 0 Rv1203c Rv1203c Unknown Hypothetical protein Rv1203c, (MTCI364.15c), len: 194 aa. Hypothetical unknown protein. O05304 No conserved hypotheticals O05304 O05304 Mb1235c NC_000962.3 Mycobrowser_v4 CDS 1348719 1349282 . + 0 Rv1205 Rv1205 Function unknown Conserved hypothetical protein Rv1205, (MTCI364.17), len: 187 aa. Conserved hypothetical protein, similar to Q49952 cosmid B1756 from Mycobacterium leprae (187 aa), FASTA scores: opt: 865, E(): 0, (72.4% identity in 174 aa overlap), also similar to FAS6_RHOFA|P46378 hypothetical 21.1 kDa protein in fasciation locus (ORF6) (198 aa), FASTA scores: opt: 368, E(): 1.3e-17, (37.4% identity in 174 aa overlap). Some similarity to YJL055W Hypothetical protein in BTN1-PEP8 intergenic region from Saccharomyces cerevisiae and P48636 hypothetical protein in AZU 5'region from Pseudomonas aeruginosa. The transcription of this CDS seems to be activated specifically in host granulomas (see citation below). O05306 No conserved hypotheticals O05306 O05306 O05306 ML1061 MMAR_4233 MSMEG_5087 Mb1237 NC_000962.3 Mycobrowser_v4 CDS 1351191 1352147 . + 0 Rv1207 folP2 Involved in dihydrofolate biosynthesis [catalytic activity: 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate] Dihydropteroate synthase 2 FolP2 (DHPS 2) (dihydropteroate pyrophosphorylase 2) Rv1207, (MTCI364.19), len: 318 aa. folP2, Dihydropteroate synthase 2, similar to many e.g. DHPS_ECOLI|P26282 Escherichia coli (282 aa), FASTA scores: opt: 480, E(): 1.9e-22, (34.4% identity in 270 aa overlap). Contains PS00792 dihydropteroate synthase signature 1, PS00793 dihydropteroate synthase signature 2. P9WNC9 No intermediary metabolism and respiration 2VP8 P64139 P9WNC9 GO:0046656,GO:0046677,GO:0004156 2.5.1.15 P9WNC9 ML1063 MMAR_4231 MSMEG_5085 Mb1239 NC_000962.3 Mycobrowser_v4 CDS 1352144 1353118 . + 0 Rv1208 gpgS Glucosylglycerate biosynthesis [catalytic activity: NDP-glucose + 3-phosphoglycerate = glucosyl-3-phsophoglycerate + NDP] Probable glucosyl-3-phosphoglycerate synthase GpgS Rv1208, (MTCI364.20), len: 324 aa. Probable gpgS, glucosyl-3-phosphoglycerate synthase (See Empadinhas et al., 2008), similar to Q49955|U1756L Mycobacterium leprae (318 aa), FASTA scores, opt: 1621, E(): 0, (80.5% identity in 318 aa overlap). Belongs to retaining glycosyltransferase family 81. P9WMW9 No intermediary metabolism and respiration 3E25,3E26 O05309 P9WMW9 P9WMW9 ML1064 MMAR_4230 MSMEG_5084 Mb1240 NC_000962.3 Mycobrowser_v4 CDS 1353522 1354136 . + 0 Rv1210 tagA Involved in base excision repair. Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions Probable DNA-3-methyladenine glycosylase I TagA (tag I) (3-methyladenine-DNA glycosylase I, constitutive) (DNA-3-methyladenine glycosidase I) Rv1210, (MTCI364.22), len: 204 aa. Probable tagA, DNA-3-methyladenine glycosidase I (see citation below), similar to several e.g. 3MG1_ECOLI|P05100 DNA-3-methyladenine glycosidase I from Escherichia coli (187 aa), FASTA scores: opt: 530, E(): 1.3e-27, (44.2% identity in 190 aa overlap); similar to Q49957 Mycobacterium leprae cosmid B1756 (192 aa), FASTA scores: opt: 1042, E(): 0, (80.2% identity in 192 aa overlap). O05311 No information pathways O05311 O05311 GO:0006284,GO:0008725 3.2.2.20 O05311 ML1066 MMAR_4228 MSMEG_5082 Mb1242 NC_000962.3 Mycobrowser_v4 CDS 1354243 1354470 . + 0 Rv1211 Rv1211 Function unknown Conserved protein Rv1211, (MTCI364.23), len: 75 aa. Conserved protein, similar to Q49958|U1756N Mycobacterium leprae (75 aa), FASTA scores: opt: 460, E(): 0, (90.7% identity in 75 aa overlap). O05312 No conserved hypotheticals O05312 O05312 O05312 ML1067 MSMEG_5081 Mb1243 NC_000962.3 Mycobrowser_v4 CDS 1354498 1355661 . - 0 Rv1212c glgA Function unknown; probably involved in cellular metabolism Putative glycosyl transferase GlgA Rv1212c, (MTCI364.24c), len: 387 aa. Putative glgA, glycosyl transferase, highly similar to AJ243803|SCO243803_2 Putative glycosyl transferase from Streptomyces coelicolor (387 aa), FASTA scores: opt: 1344, E(): 0, (54.9% identity in 388 aa overlap). Also similar to MJ1607 probable hexosyltransferase from Methanococcus jannaschii (390 aa), FASTA scores: opt: 445, E(): 7.8e-23, (27.9% identity in 401 aa overlap). The region from aa 267-355 highly similar to Q49959 cosmid B1756 from Mycobacterium leprae (91 aa), FASTA scores, opt: 471, E(): 4.8e-25, (80.9% identity in 89 aa overlap). Similar to Mycobacterium tuberculosis hypothetical protein, Rv3032. P9WMZ1 No intermediary metabolism and respiration O05313 P9WMZ1 GO:0016740,GO:0009058 2.-.-.- P9WMZ1 ML1068,ML1068c MMAR_4226 MSMEG_5080 Mb1244c NC_000962.3 Mycobrowser_v4 CDS 1363503 1364150 . - 0 Rv1220c Rv1220c Function unknown; involved in cellular metabolism Probable methyltransferase Rv1220c, (MTCI61.03c), len: 215 aa. Possible methyltransferase, some similarity to MDMC_STRMY|Q00719 o-methyltransferase from Streptomyces mycarofaciens (221 aa), FASTA scores; opt: 289, E(): 1.3e-07, (30.0% identity in 203 aa overlap). Also similar to Mycobacterium tuberculosis methyltransferases Rv0187|MTCI28.26 (32.9% identity in 222 aa overlap) and Rv1703c. Start site chosen by homology; other possible start sites exist upstream. P9WJZ7 No intermediary metabolism and respiration O33219 P9WJZ7 GO:0008171 2.1.1.- P9WJZ7 ML1075,ML1075c MMAR_4217 MSMEG_5073 Mb1252c NC_000962.3 Mycobrowser_v4 CDS 1374322 1374864 . - 0 Rv1231c Rv1231c Unknown Probable membrane protein Rv1231c, (MTV006.03c), len: 180 aa. Probable membrane protein, similar to others e.g. AL390975 Streptomyces coelicolor (198 aa). O86314 No cell wall and cell processes O86314 O86314 ML1082,ML1082c MMAR_4210 MSMEG_5066 Mb1263c NC_000962.3 Mycobrowser_v4 CDS 2259326 2259820 . + 0 Rv2012 Rv2012 Unknown Conserved hypothetical protein Rv2012, (MTCY39.05c), len: 164 aa. Conserved hypothetical protein. P9WLM3 No conserved hypotheticals P9WLM3 P9WLM3 Mb2035 NC_000962.3 Mycobrowser_v4 ncRNA 1365274 1365365 . + 0 MTB000062 mpr6 Unknown Fragment of putative small regulatory RNA mpr6, fragment of putative small regulatory RNA (See DiChiara et al., 2010), ends not mapped, ~118 nt band detected by Northern blot in M. bovis BCG Pasteur. No stable RNAs NC_000962.3 Mycobrowser_v4 ncRNA 1413094 1413224 . - 0 MTB000063 mcr11 Unknown Putative small regulatory RNA mcr11, putative small regulatory RNA (See DiChiara et al., 2010). 5'-end mapped by RLM-RACE in M. tuberculosis H37Rv, 3'-end not mapped (See Arnvig et al., 2011). No stable RNAs NC_000962.3 Mycobrowser_v4 -10_signal 1413232 1413237 . - 0 MTB000063 mcr11 Unknown Putative small regulatory RNA mcr11, putative small regulatory RNA (See DiChiara et al., 2010). 5'-end mapped by RLM-RACE in M. tuberculosis H37Rv, 3'-end not mapped (See Arnvig et al., 2011). No stable RNAs NC_000962.3 Mycobrowser_v4 -35_signal 1413255 1413260 . - 0 MTB000063 mcr11 Unknown Putative small regulatory RNA mcr11, putative small regulatory RNA (See DiChiara et al., 2010). 5'-end mapped by RLM-RACE in M. tuberculosis H37Rv, 3'-end not mapped (See Arnvig et al., 2011). No stable RNAs NC_000962.3 Mycobrowser_v4 CDS 1361798 1362733 . - 0 Rv1218c Rv1218c Thought to be involved in active transport of tetronasin across the membrane (export): tetronasin resistance by an export mechanism. Responsible for energy coupling to the transport system. Probable tetronasin-transport ATP-binding protein ABC transporter Rv1218c, (MTCI61.01c), len: 311 aa. Probable tetronasin-transport ATP-binding protein ABC transporter (see citation below), similar to many e.g. Q54406|X73633|TNRB2 TNRB2 protein from Streptomyces longisporoflavus (300 aa), FASTA scores: opt: 1133, E(): 0, (60.8% identity in 291 aa overlap); etc. Also similar to others in Mycobacterium tuberculosis e.g. MTCY19H9.04 (30.0% identity in 297 aa overlap); etc. Contains PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters). O86311 No cell wall and cell processes O86311 O86311 GO:0016887,GO:0005524 O86311 ML1073,ML1073c MMAR_4220 Mb1250c NC_000962.3 Mycobrowser_v4 CDS 1362723 1363361 . - 0 Rv1219c Rv1219c Involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv1219c, (MTCI61.02c), len: 212 aa. Probable transcriptional regulatory protein, some similarity in N-terminus to YBIH_ECOLI|P41037 hypothetical transcriptional regulator from Escherichia coli (103 aa), FASTA scores: opt: 143, E(): 8.9e-06, (39.7% identity in 63 aa overlap); Helix turn helix motif from aa 28-49. O86312 No regulatory proteins O86312 O86312 GO:0006350,GO:0003700,GO:0006355 O86312 ML1074,ML1074c MMAR_4219 MSMEG_5074 Mb1251c NC_000962.3 Mycobrowser_v4 -10_signal 1363369 1363374 . - 0 Rv1219c Rv1219c Involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv1219c, (MTCI61.02c), len: 212 aa. Probable transcriptional regulatory protein, some similarity in N-terminus to YBIH_ECOLI|P41037 hypothetical transcriptional regulator from Escherichia coli (103 aa), FASTA scores: opt: 143, E(): 8.9e-06, (39.7% identity in 63 aa overlap); Helix turn helix motif from aa 28-49. O86312 No regulatory proteins O86312 O86312 GO:0006350,GO:0003700,GO:0006355 O86312 ML1074,ML1074c MMAR_4219 MSMEG_5074 Mb1251c NC_000962.3 Mycobrowser_v4 -35_signal 1363392 1363397 . - 0 Rv1219c Rv1219c Involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv1219c, (MTCI61.02c), len: 212 aa. Probable transcriptional regulatory protein, some similarity in N-terminus to YBIH_ECOLI|P41037 hypothetical transcriptional regulator from Escherichia coli (103 aa), FASTA scores: opt: 143, E(): 8.9e-06, (39.7% identity in 63 aa overlap); Helix turn helix motif from aa 28-49. O86312 No regulatory proteins O86312 O86312 GO:0006350,GO:0003700,GO:0006355 O86312 ML1074,ML1074c MMAR_4219 MSMEG_5074 Mb1251c NC_000962.3 Mycobrowser_v4 CDS 1413260 1413940 . + 0 Rv1265 Rv1265 Unknown. Seems to be expressed during macrophage infection. Unknown protein Rv1265, (MTCY50.17c), len: 226 aa. Unknown protein (see citation below). P9WM49 No conserved hypotheticals P9WM49 ML1112 MMAR_4172 MSMEG_5010 Mb1296 NC_000962.3 Mycobrowser_v4 CDS 1365344 1365808 . + 0 Rv1222 rseA Regulates negatively SIGE|Rv1221 Anti-sigma factor RseA Rv1222, (MTCI61.05), len: 154 aa. RseA, anti-sigma factor (See Dona et al., 2008). Identical to O06290|MTU87242 (but shorter due to different start site chosen by proximity of RBS). Equivalent to O05736|U87308|MAU87308_2 hypothetical protein from Mycobacterium avium (133 aa), FASTA scores: opt: 644, E(): 7e-32, (86.2% identity in 109 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). L0T905 No information pathways L0T905 ML1077 MMAR_4215 MSMEG_5071 Mb1254 NC_000962.3 Mycobrowser_v4 CDS 1367463 1367858 . + 0 Rv1224 tatB Involved in proteins export. This sec-independent pathway is termed tat for twin-arginine translocation system. This system mainly transports proteins with bound cofactors that require folding prior to export. Probable protein TatB Rv1224, (MTCI61.07), len: 131 aa. Probable tatB, component of twin-arginine translocation protein export system (see citation below). Possible exported protein with hydrophobic stretch at N-terminus. Highly similar to Q49973|U15180 hypothetical protein U1756Y from Mycobacterium leprae (120 aa), FASTA scores: opt: 601, E(): 0, (73.3% identity in 131 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WG99 No cell wall and cell processes O33220 P9WG99 GO:0005886,GO:0009306,GO:0043953,GO:0008565,GO:0055085,GO:0016021 P9WG99 ML1079 MMAR_4213 MSMEG_5069 Mb1256 NC_000962.3 Mycobrowser_v4 CDS 1368832 1370295 . - 0 Rv1226c Rv1226c Unknown Probable transmembrane protein Rv1226c, (MTCI61.09c), len: 487 aa. Probable transmembrane protein. Some similarity to AL049841|SCE9.01 Streptomyces coelicolor (436 aa), FASTA scores: opt: 203, E(): 1.2e-05, (29.8% identity in 346 aa overlap). O33222 No cell wall and cell processes O33222 O33222 GO:0016021 Mb1258c NC_000962.3 Mycobrowser_v4 CDS 1370292 1370825 . - 0 Rv1227c Rv1227c Unknown Probable transmembrane protein Rv1227c, (MTCI61.10c), len: 177 aa. Possible transmembrane protein, similar to P96615 hypothetical protein ydbS from Bacillus subtilis (159 aa), fasta scores: E(): 3.6e-07, (30.1% identity in 163 aa overlap). O33223 No cell wall and cell processes O33223 O33223 GO:0016021 MSMEG_0864 Mb1259c NC_000962.3 Mycobrowser_v4 CDS 1371777 1372949 . - 0 Rv1229c mrp Unknown: thought to be a ATP-binding protein. Probable Mrp-related protein Mrp Rv1229c, (MT1267, MTCI61.12c, MTV006.01c), len: 390 aa. Probable Mrp protein, similar to others e.g. MRP_ECOLI|P21590 mrp protein from Escherichia coli (379 aa), FASTA scores: E(): 0, (34.1% identity in 355 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop); and PS01215 MRP Prosite domain. Belongs to the MRP/NBP35 family of ATP-binding proteins. P9WJN7 No intermediary metabolism and respiration P65441 P9WJN7 GO:0005524 P9WJN7 ML1080,ML1080c MMAR_4212 MSMEG_5068 Mb1261c NC_000962.3 Mycobrowser_v4 CDS 1372962 1374197 . - 0 Rv1230c Rv1230c Unknown Possible membrane protein Rv1230c, (MTV006.02c), len: 411 aa. Possible membrane protein with two hydrophobic stretches near N-terminus. Some similarity to Rv1022|MTCY10G2.27c|Z92539 probable lpqU protein Mycobacterium tuberculosis (243 aa), FASTA score: opt: 408, E(): 1e-11, (43.6% identity in 172 aa overlap). Similar to AL133423|SC4A7.37 hypothetical protein from Streptomyces coelicolor (421 aa), FASTA score: opt: 679, E(): 5.1e-23, (36.4% identity in 398 aa overlap). O86313 No cell wall and cell processes O86313 GO:0003700,GO:0006355 O86313 ML1081,ML1081c MMAR_4211 MSMEG_5067 Mb1262c NC_000962.3 Mycobrowser_v4 CDS 1376230 1376826 . - 0 Rv1233c Rv1233c Function unknown Conserved hypothetical membrane protein Rv1233c, (MTV006.05c), len: 198 aa. Conserved hypothetical membrane protein, N-terminus is highly proline rich, C-terminus has two hydrophobic stretches. Proline-rich N-terminus has some similarity to CBPA_DICDI calcium binding protein from Dictyostelium discoideum (467 aa), FASTA scores: E(): 4.8e-06, (35.5% identity in 183 aa overlap). Both sequences share multiple copies of a Tyr-Pro-Pro motif. O86316 No cell wall and cell processes O86316 ML1084,ML1084c Mb1265c NC_000962.3 Mycobrowser_v4 CDS 1376976 1377503 . + 0 Rv1234 Rv1234 Unknown Probable transmembrane protein Rv1234, (MTV006.06), len: 175 aa. Possible transmembrane protein with two TM helices. O50451 No cell wall and cell processes O50451 GO:0016021 ML1085 MMAR_4207 MSMEG_5062 Mb1266 NC_000962.3 Mycobrowser_v4 CDS 1378927 1379850 . + 0 Rv1236 sugA Involved in active transport of sugar across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable sugar-transport integral membrane protein ABC transporter SugA Rv1236, (MTV006.08), len: 307 aa. Probable sugA, sugar-transport integral membrane protein ABC transporter (see citation below), equivalent to U15180|MLU1518035 protein malFM from Mycobacterium leprae (310 aa), FASTA scores: opt: 1566, E(): 0, (81.8% identity in 292 aa overlap). Also similar to numerous bacterial sugar transport system components. Also similar to Rv2316|MTCY3G12.18c from Mycobacterium tuberculosis (290 aa), FASTA scores: opt: 514, E(): 7.3e-27, (33.2% identity in 283 aa overlap). Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature. P9WG03 No cell wall and cell processes O50452 P9WG03 GO:0005886,GO:0006810,GO:0005215,GO:0016021 P9WG03 ML1087 MMAR_4205 MSMEG_5060 Mb1268 NC_000962.3 Mycobrowser_v4 CDS 1379855 1380679 . + 0 Rv1237 sugB Involved in active transport of sugar across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable sugar-transport integral membrane protein ABC transporter SugB Rv1237, (MTV006.09), len: 274 aa. Probable sugB, sugar-transport integral membrane protein ABC transporter (see citation below), equivalent to U15180|MLU1518036 protein MalGM from Mycobacterium leprae (296 aa), FASTA scores: opt: 1571, E(): 0, (89.8% identity in 274 aa overlap). Also similar to numerous bacterial sugar transport protein. Related to Rv2834c|MTCY16B7.08 from Mycobacterium tuberculosis (275 aa), FASTA scores: opt: 370, E(): 2.4e-17, (26.8% identity in 269 aa overlap). P9WG01 No cell wall and cell processes O50453 P9WG01 GO:0005886,GO:0006810,GO:0005215,GO:0016021 P9WG01 ML1088 MMAR_4204 MSMEG_5059 Mb1269 NC_000962.3 Mycobrowser_v4 CDS 1456565 1457542 . + 0 Rv1300 hemK Possibly involved in the oxidation of protoporphyrinogen into protoporphyrin IX Probable HemK protein homolog HemK Rv1300, (MTCY373.20), len: 325 aa. Probable hemK protein homolog, homology suggests translation may start at aa 22, highly similar to many e.g. HEMK_MYCLE|P45832 Mycobacterium leprae (288 aa), FASTA scores: opt: 936, E(): 0, (76.7% identity in 189 aa overlap). Belongs to the HemK family of modification methylases. P9WHV3 No intermediary metabolism and respiration Q10602 P9WHV3 GO:0006479,GO:0008276,GO:0003676 2.1.1.- P9WHV3 ML1135 MMAR_4097 MSMEG_4949 Mb1332 NC_000962.3 Mycobrowser_v4 CDS 1686570 1686887 . + 0 Rv1495 mazF4 Sequence-specific mRNA cleavage Possible toxin MazF4 Rv1495, (MTCY277.17), len: 105 aa. Possible mazF4, toxin, part of toxin-antitoxin (TA) operon with Rv1494 (See Pandey and Gerdes, 2005; Zhu et al., 2006), some similarity to Rv1942c|MTCY09F9.22 hypothetical protein from Mycobacterium tuberculosis (109 aa) (0.7% identity in 101 aa overlap) and Rv0659c, Rv1102c. P9WII5 No virulence, detoxification, adaptation P64859 P9WII5 GO:0003677 P9WII5 Mb1532 NC_000962.3 Mycobrowser_v4 CDS 1384278 1384538 . + 0 Rv1241 vapB33 Unknown Possible antitoxin VapB33 Rv1241, (MTV006.13), len: 86 aa. Possible vapB33, antitoxin, part of toxin-antitoxin (TA) operon with Rv1242, see Arcus et al. 2005. Member of family of 16 hypothetical Mycobacterium tuberculosis proteins including: Rv2871|Q10799|YS71_MYCTU hypothetical 13.2 kDa protein CY2 (124 aa), FASTA scores: opt: 172, E(): 9.5e-06, (37.2% identity in 86 aa overlap); Rv2132, Rv3321c, etc. O50456 No virulence, detoxification, adaptation O50456 O50456 Mb1273 NC_000962.3 Mycobrowser_v4 CDS 1381942 1383042 . - 0 Rv1239c corA Thought to be involved in transport of magnesium and cobalt ions across the membrane. Responsible for the translocation of the substrate across the membrane. Possible magnesium and cobalt transport transmembrane protein CorA Rv1239c, (MTV006.11c), len: 366 aa. Possible corA, magnesium and cobalt transport transmembrane protein, highly similar to U15180 corA protein from Mycobacterium leprae (373 aa), FASTA scores: opt: 1985, E(): 0, (79.1% identity in 369 aa overlap). Also similar to various CorA proteins of Gram negative bacteria e.g. P27841|CORA_ECOLI|B3816|Z5333|ECS4746 Magnesium and cobalt transport protein from Escherichia coli strains K12 and O157:H7 (316 aa), FASTA scores: opt: 236, E(): 8e-08, (24.5% identity in 306 aa overlap); etc. Seems to belong to the MIT family. O50455 No cell wall and cell processes O50455 O50455 GO:0006824,GO:0016021,GO:0015095,GO:0015693,GO:0055085,GO:0015087 O50455 ML1090,ML1090c MMAR_4199 MSMEG_5056 Mb1271c NC_000962.3 Mycobrowser_v4 CDS 1383213 1384202 . + 0 Rv1240 mdh Involved in the conversion of malate to oxaloacetate [catalytic activity: (S)-malate + NAD+ = oxaloacetate + NADH]. Probable malate dehydrogenase Mdh Rv1240, (MTV006.12), len: 329 aa. Probable mdh, Malate dehydrogenase. Most similar to P50917|MDH_MYCLE malate dehydrogenase from Mycobacterium leprae (329 aa), FASTA scores: opt: 1887, E(): 0, (89.1% identity in 329 aa overlap). Contains PS00068 Malate dehydrogenase active site signature. Belongs to the LDH family. MDH subfamily. P9WK13 No intermediary metabolism and respiration P0A5J6 P9WK13 GO:0006096,GO:0006108,GO:0055114,GO:0006099,GO:0030060 1.1.1.37 P9WK13 ML1091 MMAR_4198 Mb1272 NC_000962.3 Mycobrowser_v4 CDS 1384535 1384966 . + 0 Rv1242 vapC33 Unknown Possible toxin VapC33. Contains PIN domain. Rv1242, (MTV006.14), len: 143 aa. Possible vapC33, toxin, part of toxin-antitoxin (TA) operon with Rv1241, contains PIN domain, see Arcus et al. 2005. Member of family of 14 hypothetical Mycobacterium tuberculosis proteins including: Rv2872|Q10800|YS72_MYCTU (147 aa), FASTA scores: opt: 226, E(): 2.7e-09, (32.1% identity in 137 aa overlap); Rv0749, Rv0277c, Rv2530c, etc. P9WF69 No virulence, detoxification, adaptation P9WF69 P9WF69 Mb1274 NC_000962.3 Mycobrowser_v4 CDS 1384989 1386677 . - 0 Rv1243c PE_PGRS23 Function unknown PE-PGRS family protein PE_PGRS23 Rv1243c, (MTV006.15c), len: 562 aa. PE_PGRS23, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002). Q79FQ7 No PE/PPE Q79FQ7 Q79FQ7 Q79FQ7 ML1092,ML1092c Mb1275c NC_000962.3 Mycobrowser_v4 CDS 1386857 1387717 . + 0 Rv1244 lpqZ Unknown Probable lipoprotein LpqZ Rv1244, (MTV006.16), len: 286 aa. Probable lipoprotein lpqZ, equivalent toU15180|MLU1518042 protein u1756x from Mycobacterium leprae (228 aa), FASTA scores: opt: 1039, E(): 0, (72.5% identity in 229 aa overlap). Similar to Mycobacterium tuberculosis hypothetical protein Rv3759c. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O50459 No cell wall and cell processes O50459 O50459 GO:0006810,GO:0005215 O50459 ML1093 MMAR_4196 MSMEG_5054 Mb1276 NC_000962.3 Mycobrowser_v4 CDS 1699866 1700228 . + 0 Rv1508A Rv1508A Function unknown Conserved hypothetical protein Rv1508A, len: 120 aa. Conserved hypothetical protein, highly similar to central part of glycosyl transferases from various mycobacteria and eubacteria e.g. P71790|MTCY277.33|Rv1511 Hypothetical protein from M. tuberculosis (340 aa), FASTA scores: opt: 210, E(): 2.5 e-09, (42.9% identity in 105 aa overlap). Q79FN0 No conserved hypotheticals Q79FN0 GO:0019673,GO:0005622,GO:0008446 Q79FN0 NC_000962.3 Mycobrowser_v4 CDS 2200938 2201249 . + 0 Rv1953 vapC14 Unknown Possible toxin VapC14 Rv1953, (MTCY09F9.11c), len: 103 aa. Possible vapC14, toxin, part of toxin-antitoxin (TA) operon with Rv1952, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Some similarity to O33827 plasmid stability-like protein from Thiobacillus ferrooxidans (143 aa), FASTA scores: opt: 170, E(): 3.5e-06, (45.3% identity in 75 aa overlap). P9WF99 No virulence, detoxification, adaptation P95261 P9WF99 Mb1988 NC_000962.3 Mycobrowser_v4 CDS 1388685 1388978 . - 0 Rv1246c relE Function unknown Toxin RelE Rv1246c, (MTV006.18c), len: 97 aa. RelE, toxin, part of toxin-antitoxin (TA) operon with Rv1247c (See Pandey and Gerdes, 2005), highly similar to Rv2866|MTV003.12 hypothetical Mycobacterium tuberculosis protein (87 aa), FASTA scores: opt: 290, E(): 3.9e-24, (54.1% identity in 85 aa overlap). O50461 No virulence, detoxification, adaptation O50461 O50461 O50461 Mb1278c NC_000962.3 Mycobrowser_v4 CDS 1388975 1389244 . - 0 Rv1247c relB Function unknown Antitoxin RelB Rv1247c, (MTV006.19c), len: 89 aa. RelB, antitoxin, part of toxin-antitoxin (TA) operon with Rv1246c (See Pandey and Gerdes, 2005), some similarity to hypothetical proteins including Mycobacterium tuberculosis proteins Rv2865|MTV003.11 (93 aa), FASTA scores: opt: 249, E(): 5.4e-13, (44.2% identity in 86 aa overlap); Rv0268|Z86089|P95225 (169 aa) opt: 125, E(): 0.0089, (41.8% identity in 55 aa overlap); etc. and Escherichia coli AE000293|ECAE0002933 (92 aa), FASTA scores: opt: 127, E(): 0.0038, (29.3% identity in 82 aa overlap). O50462 No virulence, detoxification, adaptation O50462 O50462 O50462 Mb1279c NC_000962.3 Mycobrowser_v4 CDS 1393194 1393982 . - 0 Rv1249c Rv1249c Unknown Possible membrane protein Rv1249c, (MTV006.21c), len: 262 aa. Possible membrane protein. Start uncertain. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O50464 No cell wall and cell processes O50464 ML1096,ML1096c MMAR_4193 MSMEG_5048 Mb1281c NC_000962.3 Mycobrowser_v4 CDS 1399970 1401661 . + 0 Rv1253 deaD Has a helix-destabilizing activity Probable cold-shock DeaD-box protein A homolog DeaD (ATP-dependent RNA helicase dead homolog) Rv1253, (MTCY50.29c), len: 563 aa. Probable deaD, Cold-shock dead-box protein A homolog, similar to many e.g. DEAD_ECOLI|P23304 Escherichia coli (646 aa), FASTA scores: opt: 1490, E(): 0, (46.7% identity in 578 aa overlap); similar to Mycobacterium tuberculosis Rv3211. Contains PS00017 ATP/GTP-binding site motif A, PS00039 dead-box subfamily ATP-dependent helicases signature. Belongs to the dead box family helicase. P9WH05 No information pathways Q11039 P9WH05 GO:0008026,GO:0003723,GO:0005737,GO:0045449,GO:0006350,GO:0005524 3.6.4.13 P9WH05 ML1100 MMAR_4189 MSMEG_5042 Mb1285 NC_000962.3 Mycobrowser_v4 CDS 1700212 1701093 . + 0 Rv1509 Rv1509 Unknown Hypothetical protein Rv1509, (MTCY277.31), len: 293 aa. Hypothetical unknown protein. P9WLW3 No unknown P71788 P9WLW3 GO:0008168,GO:0008152 P9WLW3 NC_000962.3 Mycobrowser_v4 CDS 1764755 1764973 . + 0 Rv1560 vapB11 Unknown Possible antitoxin VapB11 Rv1560, (MTCY48.05c), len: 72 aa. Possible vapB11, antitoxin, part of toxin-antitoxin (TA) operon with Rv1561 (See Arcus et al., 2005; Pandey and Gerdes, 2005). Part of a Mycobacterial tuberculosis family of proteins e.g. Q10848|Rv2009|MTCY39.08c (80 aa), FASTA score: (54.4% identity in 68 aa overlap); Q10799|Rv2871|MTCY274.02 (85 aa); O50456|Rv1241|MTV006.13 (86 aa), O06243|Rv2132|MTCY270.36C (76 aa); etc. P9WLU3 No virulence, detoxification, adaptation P64877 P9WLU3 P9WLU3 Mb1586 NC_000962.3 Mycobrowser_v4 CDS 1395821 1399240 . - 0 Rv1251c Rv1251c Function unknown Conserved hypothetical protein Rv1251c, (MTV006.23c), len: 1139 aa. Conserved hypothetical protein, showing some similarity in C-terminal region with other proteins from eukaryotes and bacteria e.g. NP_142121.1 hypothetical protein from Pyrococcus horikoshii (1188 aa); and some similarity to GTP-binding proteins e.g. P23249|MV10_MOUSE putative GTP-binding protein (1004 aa), FASTA scores: opt: 228, E(): 1.7e-06, (27.7% identity in 560 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O50466 No conserved hypotheticals O50466 O50466 GO:0017111,GO:0005524 O50466 ML1098,ML1098c MMAR_4191 MSMEG_5044 Mb1283c NC_000962.3 Mycobrowser_v4 CDS 1399296 1399904 . - 0 Rv1252c lprE Unknown Probable lipoprotein LprE Rv1252c, (MTCY50.30), len: 202 aa. Probable lipoprotein lprE, some similarity to Mycobacterium tuberculosis protein Rv3483c|MTCY13E12.36C (220 aa), FASTA scores: E(): 7e-05, (29.5% identity in 200 aa overlap). Contains possible N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein lipid attachment site (PS00013). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WK49 No cell wall and cell processes P9WK49 GO:0005886 ML1099,ML1099c MMAR_4190 MSMEG_5043 Mb1284c NC_000962.3 Mycobrowser_v4 CDS 1401658 1402809 . + 0 Rv1254 Rv1254 Catalyzes the acylation of the mycaminose sugar during midecamycin biosynthesis Probable acyltransferase Rv1254, (MTCY50.28c), len: 383 aa. Probable Acyltransferase, similar to G927228 midecamycin 4-0-propionyl transferase (fragment) (388 aa), FASTA scores, opt: 305, E(): 5.6e-14, (28.4% identity in 377 aa overlap). Also similar to other Mycobacterium tuberculosis acyltransferases e.g. Rv0111, Rv0228, etc. Contains PS00881 Protein splicing signature. Q11064 No intermediary metabolism and respiration Q11064 Q11064 GO:0016020,GO:0008415 2.3.1.- Q11064 ML1101 MMAR_4188 MSMEG_5041 Mb1286 NC_000962.3 Mycobrowser_v4 CDS 1403386 1404603 . - 0 Rv1256c cyp130 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Probable cytochrome P450 130 Cyp130 Rv1256c, (MT1295, MTCY50.26), len: 405 aa. Probable cyp130, cytochrome P450, similar to other cytochromes P-450 e.g. S51594 cytochrome P450 mycG from Micromonospora griseorubida (397 aa); T36526 probable cytochrome P450 hydroxylase from Streptomyces coelicolor (411 aa); CPXK_SACER|P33271|107B1 cytochrome P450 from Saccharopolyspora erythraea (405 aa), FASTA scores: opt: 639, E(): 2.7e-33, (33.2% identity in 391 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. Rv0766c|MTCY369.11c cytochrome P450 (402 aa); etc. Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. Belongs to the cytochrome P450 family. P9WPN5 No intermediary metabolism and respiration 2UUQ,2UVN,2WGY,2WH8,2WHF Q11062 P9WPN5 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPN5 ML1102,ML1102c MMAR_4184 MSMEG_5038 NC_000962.3 Mycobrowser_v4 CDS 1404717 1406084 . - 0 Rv1257c Rv1257c Function unknown; probably involved in cellular metabolism Probable oxidoreductase Rv1257c, (MTCY50.25), len: 455 aa. Probable oxidoreductase, similar to e.g. GLCD_ECOLI|P52075 glycolate oxidase subunit glcd (499 aa), FASTA scores: E(): 0, (38.9% identity in 458 aa overlap). Similar to Mycobacterium tuberculosis oxidoreductases e.g. Rv3107c Q11061 No intermediary metabolism and respiration Q11061 Q11061 GO:0055114,GO:0016491,GO:0050660 1.-.-.- Q11061 ML1103,ML1103c MMAR_4183 MSMEG_5037 NC_000962.3 Mycobrowser_v4 CDS 1407339 1408238 . + 0 Rv1259 udgB Involved in base excision repair. Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. Probable uracil DNA glycosylase, UdgB Rv1259, (MTCY50.23c), len: 299 aa. Probable udgB, uracil DNA glycosylase. Similar to AL109732|SC7H2.04 hypothetical protein from Streptomyces coelicolor (237 aa), FASTA scores: opt: 870, E(): 0, (57.1% identity in 231 aa overlap). P9WM53 No information pathways P64785 P9WM53 P9WM53 ML1105 MMAR_4181 MSMEG_5031 Mb1289 NC_000962.3 Mycobrowser_v4 CDS 1409484 1409933 . - 0 Rv1261c Rv1261c Function unknown Conserved protein Rv1261c, (MTCY50.21), len: 149 aa. Conserved protein, similar to Mycobacterium tuberculosis hypothetical proteins e.g. Rv1558|MTCY48.07c (39.2% identity in 125 aa overlap); Rv3547 and Rv3178. P9WP13 No conserved hypotheticals P64787 P9WP13 P9WP13 MMAR_4178 MSMEG_5030 Mb1292c NC_000962.3 Mycobrowser_v4 CDS 1409938 1410372 . - 0 Rv1262c Rv1262c Unknown Hypothetical hit-like protein Rv1262c, (MTCY50.20), len: 144 aa. Hypothetical hit-like protein, similar to Q04344|HIT_YEAST hit1 protein (orf u) (144 aa), FASTA scores: opt: 306, E(): 3e-14, (35.9 % identity in 142 aa overlap); also similar to YHIT_MYCGE|P47378 hypothetical 15.6 kDa protein (141 aa), FASTA scores: opt: 250, E(): 1.6e-10, (35.5% identity in 107 aa overlap); and YHIT_MYCLE|P49774 hypothetical 17.0 kDa protein hit-like (155 aa), FASTA scores: opt: 196, E(): 7e-07, (30.6% identity in 144 aa overlap). Similar to other proteins from Mycobacterium tuberculosis e.g. Rv2613c, Rv0759c. Contains PS00892 hit family signature. Belongs to the hit family. P9WML1 No conserved hypotheticals Q11066 P9WML1 GO:0003824 P9WML1 ML1107,ML1107c MMAR_4177 MSMEG_5028 Mb1293c NC_000962.3 Mycobrowser_v4 CDS 1410431 1411819 . + 0 Rv1263 amiB2 Involved in cellular metabolism, active on 2- to 6- carbon aliphatic amides and on many aromatic amides [catalytic activity: a monocarboxylic acid amide + H(2)O = a monocarboxylate + NH(3)]. Probable amidase AmiB2 (aminohydrolase) Rv1263, (MTCY50.19c), len: 462 aa. Probable amiB2, amidase. Similar to G1001278 hypothetical 54.3 kDa protein (506 aa), FASTA scores: opt: 767, E(): 7.6e-40, (32.8% identity in 461 aa overlap), also similar to G580673 rhodococcus enantiose lective amidase gene (462 aa), FASTA scores, opt: 668, E(): 7.4e-34, (33.5% identity in 484 aa overlap) also to NYLA_PSES8|P13398 6-aminohexanoate-cyclic-dimer hydrolase (492 aa), FASTA scores opt: 543, E(): 3.1e-26, (33.5% identity in 493 aa overlap). Also similar to MTCY274.19c (33.5% identity in 427 aa overlap). Similar to other putative amidases in M. tuberculosis; Rv2363, Rv2888c, etc. Contains PS00017 ATP/GTP-binding site motif A. Belongs to the amidase family. P9WQ97 No intermediary metabolism and respiration P63492 P9WQ97 GO:0016884,GO:0004040 3.5.1.4 P9WQ97 ML1109 MMAR_4176 Mb1294 NC_000962.3 Mycobrowser_v4 CDS 1508543 1508923 . - 0 Rv1343c lprD Unknown Probable conserved lipoprotein LprD Rv1343c, (MTCY02B10.07c), len: 126 aa. Probable lprD, conserved lipoprotein, highly similar to G466928 Mycobacterium leprae protein B1549_F3_106 (126 aa), FASTA scores, opt: 704, E(): 7.5e-36, (78.4 % identity in 125 aa overlap). Has N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein attachment site. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WK51 No cell wall and cell processes P9WK51 GO:0005886,GO:0016021 ML1177,ML1177c MMAR_4038 MSMEG_4898 Mb1378c NC_000962.3 Mycobrowser_v4 CDS 1416181 1417347 . - 0 Rv1267c embR Involved in transcriptional mechanism. Thought to regulate the biosynthesis of the mycobacterial cell wall arabinan and resistance to ethambutol (EMB; dextro-2,2'-(ethylenediimino)-DI-1-butanol), regulating EMBA|Rv3794 and EMBB|Rv3795. Probable transcriptional regulatory protein EmbR Rv1267c, (MT1305, MTCY50.15), len: 388 aa. Probable embR, regulatory protein (see citation below), similar to many e.g. AFSR_STRCO|P25941 regulatory protein AfsR from Streptomyces coelicolor (993 aa), FASTA scores: opt: 489, E(): 1e-25, (33.5% identity in 361 aa overlap); etc. Belongs to the AFSR/DNRI/REDD family of regulators. Phosphorylated in vitro by PknJ|Rv2088 (See Jang et al., 2010). P9WGJ9 No regulatory proteins 2FEZ,2FF4 P66799 P9WGJ9 GO:0005622,GO:0006355,GO:0006350,GO:0000156,GO:0000160,GO:0003677 EMB P9WGJ9 MMAR_4155 Mb1298c NC_000962.3 Mycobrowser_v4 CDS 1420410 1422305 . - 0 Rv1272c Rv1272c Thought to be involved in active transport of drugs across the membrane (export): multidrugs resistance by an export mechanism. Responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane. Probable drugs-transport transmembrane ATP-binding protein ABC transporter Rv1272c, (MTCY50.10), len: 631 aa. Probable drugs-transport transmembrane ATP-binding protein ABC transporter (see citation below), similar to e.g. Y015_MYCGE|P47261 hypothetical ABC transporter mg015m from Mycoplasma genitalium (589 aa), FASTA scores: opt: 1054, E(): 0, (34.3% identity in 522 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop); and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters), MSBA subfamily. P9WQJ3 No cell wall and cell processes P63397 P9WQJ3 GO:0042626,GO:0016021,GO:0005886,GO:0055085,GO:0005524 P9WQJ3 ML1113,ML1113c MMAR_4148 MSMEG_5009 Mb1303c NC_000962.3 Mycobrowser_v4 CDS 1437324 1437815 . + 0 Rv1284 canA Catalyzes reversible dehydration of CO2 to form bicarbonate Beta-carbonic anhydrase Rv1284, (MTCY373.03), len: 163 aa. CanA, Beta-carbonic anhydrase, proven biochemically (See Suarez Covarrubias et al. 2005) similar to others e.g. AL109663|SC4A10.26 hypothetical protein from Streptomyces coelicolor (167 aa), FASTA scores: opt: 567, E(): 1.5e-32, (53.4% identity in 163 aa overla); shows some similarity to hypothetical protein from Methanobacterium thermoautotrophicum. Weak similarity to carbonic anhydrases e.g. U51624|MTU516242|P17582 Methanothermobacter thermautotrophicus (171 aa), FASTA score: opt: 305, E(): 1.2e-14, (35.2% identity in 165 aa overlap). P9WPJ7 No intermediary metabolism and respiration 1YLK P64797 P9WPJ7 GO:0004089,GO:0008270,GO:0015976 P9WPJ7 ML1125 MMAR_4135 MSMEG_4985 Mb1315 NC_000962.3 Mycobrowser_v4 CDS 1457558 1458211 . + 0 Rv1301 Rv1301 Function unknown Conserved protein Rv1301, (MTCY373.21), len: 217 aa. Conserved protein, highly similar to YRFE_MYCLE|P45831 hypothetical 22.7 kDa protein in rfe-hemk intergenic region, (220 aa), FASTA scores: opt: 1168, E(): 0, (82.8% identity in 215 aa overlap). Contains PS01147 Hypothetical SUA5/yciO/yrdC family signature. Belongs to the SUA5/YRDC/YCIO/YWLC family. P9WGC9 No conserved hypotheticals Q10618 P9WGC9 P9WGC9 ML1136 MMAR_4096 MSMEG_4948 Mb1333 NC_000962.3 Mycobrowser_v4 CDS 1413960 1415840 . - 0 Rv1266c pknH Involved in signal transduction (via phosphorylation). Thought to be involved in arabinan metabolism, phosphorylating perhaps EMBR|Rv1267c [catalytic activity: ATP + a protein = ADP + a phosphoprotein]. Probable transmembrane serine/threonine-protein kinase H PknH (protein kinase H) (STPK H) Rv1266c, (MTCY50.16), len: 626 aa. Probable pknH, transmembrane serine/threonine-protein kinase (see citation below), similar to many e.g. PKN1_MYXXA|P33973 pkn1 (693 aa), FASTA scores: opt: 611, E(): 1.4e- 14, (29.7% identity in 492 aa overlap); etc. Contains PS00107 Protein kinases ATP-binding region signature; PS00108 Serine/Threonine protein kinases active-site signature. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases. Experimental studies show evidence of auto-phosphorylation. P9WI71 No regulatory proteins Q11053 P9WI71 GO:0006468,GO:0004674,GO:0005524 2.7.11.1 P9WI71 MMAR_4156,MMAR_4171 MSMEG_5513 Mb1297c NC_000962.3 Mycobrowser_v4 CDS 1424197 1424754 . + 0 Rv1274 lprB Unknown Possible lipoprotein LprB Rv1274, (MTCY50.08c), len: 185 aa. Possible lprB, lipoprotein; contains possible N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein lipid attachment site (PS00013). Some similarity to Rv1275. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WK53 No cell wall and cell processes P9WK53 GO:0005886 ML1115 MMAR_4146 MSMEG_5007 Mb1305 NC_000962.3 Mycobrowser_v4 CDS 1424751 1425293 . + 0 Rv1275 lprC Unknown Possible lipoprotein LprC Rv1275, (MTCY50.07c), len: 180 aa. Possible lprC, lipoprotein; contains possible N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein lipid attachment site (PS00013). Some similarity to Rv1274. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O86337 No cell wall and cell processes O86337 ML1116 MSMEG_5005 Mb1306 NC_000962.3 Mycobrowser_v4 CDS 1425438 1425914 . - 0 Rv1276c Rv1276c Function unknown Conserved hypothetical protein Rv1276c, (MTCY50.06), len: 158 aa. Conserved hypothetical protein, similar to AL096844|SCI28.03 hypothetical protein from Streptomyces coelicolor (172 aa), FASTA scores: opt: 385, E(): 3.3e-19, (43.5% identity in 161 aa overlap). Some similarity to P76502|SIXA_ECOLI phosphohistidine phosphatase SIXA (161 aa), FASTA scores: opt: 146, E(): 0.0047, (31.9% identity in 116 aa overlap). Belongs to the SixA family of phosphatases. P9WGF9 No conserved hypotheticals Q11043 P9WGF9 GO:0016787 3.1.3.- P9WGF9 ML1117,ML1117c MMAR_4144 MSMEG_5006 Mb1307c NC_000962.3 Mycobrowser_v4 CDS 1427414 1430041 . + 0 Rv1278 Rv1278 Unknown Hypothetical protein Rv1278, (MTCY50.04c), len: 875 aa. Hypothetical unknown protein, possible coiled-coil regions, contains PS00017 ATP/GTP-binding site motif A. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WM41 No conserved hypotheticals P9WM41 P9WM41 ML1120 MMAR_4142 MSMEG_5002 Mb1309 NC_000962.3 Mycobrowser_v4 CDS 1430062 1431648 . + 0 Rv1279 Rv1279 Function unknown; probably involved in cellular metabolism, probably electron-transfer-linked. Probable dehydrogenase FAD flavoprotein GMC oxidoreductase Rv1279, (MTCY50.03c), len: 528 aa. Probable dehydrogenase, FAD flavoprotein GMC oxidoreductase, similar to several e.g. dBETA_ECOLI|P17444 choline dehydrogenase from Escherichia coli (556 aa), FASTA scores, opt: 1047, E(): 0, (37.7% identity in 541 aa overlap). Similar to Rv0697 putative Mycobacterium tuberculosis GMC oxidoreductase. Contains PS00623 GMC oxidoreductases signature 1, and PS00624 GMC oxidoreductases signature 2. Belongs to the GMC oxidoreductases family. P9WMV5 No intermediary metabolism and respiration P64263 P9WMV5 GO:0006066,GO:0055114,GO:0016614,GO:0050660 1.1.-.- P9WMV5 MMAR_4141 Mb1310 NC_000962.3 Mycobrowser_v4 CDS 1445499 1445834 . - 0 Rv1291c Rv1291c Unknown Conserved hypothetical secreted protein Rv1291c, (MTCY373.10c), len: 111 aa. Conserved hypothetical secreted protein, similar to others in Mycobacterium tuberculosis e.g. Rv1271c|Q11048|YC71_MYCTU hypothetical 11.6 kDa protein (113 aa), FASTA score: opt: 246, E(): 1.7e-09, (40.0% identity in 110 aa overlap); Rv1804c, Rv1810, Rv0622, etc. P9WM33 No cell wall and cell processes P0A5E5 P9WM33 MMAR_4121 Mb1323c NC_000962.3 Mycobrowser_v4 CDS 1433433 1435271 . - 0 Rv1281c oppD Involved in active transport of oligopeptide across the membrane (import). Responsible for energy coupling to the transport system. Probable oligopeptide-transport ATP-binding protein ABC transporter OppD Rv1281c, (MTCY50.01), len: 612 aa. Probable oppD, oligopeptide-transport ATP-binding protein ABC transporter (see citation below), similar to others e.g. DPPD_BACSU|P26905 dipeptide transport ATP-binding protein from Bacillus subtilis (335 aa), FASTA scores: opt: 983, E(): 0, (48.6% identity in 319 aa overlap); etc. Contains 2 x PS00017 ATP/GTP-binding site motif A (P-loop); 2 x PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). P9WQJ5 No cell wall and cell processes P63395 P9WQJ5 GO:0016887,GO:0015833,GO:0015197,GO:0005524 P9WQJ5 ML1122,ML1122c MMAR_4138 MSMEG_4997 Mb1312c NC_000962.3 Mycobrowser_v4 CDS 1435268 1436143 . - 0 Rv1282c oppC Involved in active transport of oligopeptide across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable oligopeptide-transport integral membrane protein ABC transporter OppC Rv1282c, (MTCY373.01c-MTCY3H3.01), len: 291 aa. Probable oppC, oligopeptide-transport integral membrane protein ABC transporter (see Braibant et al., 2000), similar to other integral membrane proteins e.g. OPPC_ECOLI|P77664 oligopeptide transport system permease from Escherichia coli (302 aa), FASTA scores: E(): 4.6e-33, (40.7% identity in 275 aa overlap); etc. Also similar to Rv3664c|DPPC probable peptide-transport integral membrane protein from Mycobacterium tuberculosis. P9WFZ9 No cell wall and cell processes P66964 P9WFZ9 GO:0015833,GO:0005886,GO:0015031,GO:0005215,GO:0016021 ML1123,ML1123c MMAR_4137 MSMEG_4996 Mb1313c NC_000962.3 Mycobrowser_v4 CDS 1436140 1437117 . - 0 Rv1283c oppB Involved in active transport of oligopeptide across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable oligopeptide-transport integral membrane protein ABC transporter OppB Rv1283c, (MTCY373.02c), len: 325 aa. Probable oppB, oligopeptide-transport integral membrane protein ABC transporter (see citation below), similar to other integral membrane proteins e.g. DPPB_ECOLI|P37316 dipeptide transport system permease protein from Escherichia coli (339 aa), FASTA scores: opt: 402, E(): 3.4e-20, (31.0% identity in 345 aa overlap); etc. Also similar to Rv3665c|DppB probable peptide-transport integral membrane protein from Mycobacterium tuberculosis. Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature. P9WFZ7 No cell wall and cell processes P66966 P9WFZ7 GO:0015833,GO:0005886,GO:0015031,GO:0005215,GO:0016021 ML1124,ML1124c MMAR_4136 MSMEG_4995 Mb1314c NC_000962.3 Mycobrowser_v4 CDS 1446379 1448031 . + 0 Rv1292 argS Involved in translation mechanism [catalytic activity: ATP + L-arginine + tRNA(ARG) = AMP + diphosphate + L-arginyl-tRNA(ARG)]. Probable arginyl-tRNA synthetase ArgS (ARGRS) (arginine--tRNA ligase) Rv1292, (MTCY373.12), len: 550 aa. Probable argS, Arginyl-tRNA synthetase, highly similar to SYR_MYCLE|P45840 Mycobacterium leprae (550 aa), FASTA scores: opt: 3115, E(): 0, (84.9% identity in 550 aa overlap). Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Belongs to class-I aminoacyl-tRNA synthetase family. P9WFW5 No information pathways P67569 P9WFW5 GO:0004814,GO:0006420,GO:0005737,GO:0005524 6.1.1.19 P9WFW5 ML1127 MMAR_4105 MSMEG_4959 Mb1324 NC_000962.3 Mycobrowser_v4 CDS 1508968 1509288 . + 0 Rv1344 mbtL Thought to be involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins Acyl carrier protein (ACP) MbtL Rv1344, (MTCY02B10.08), len: 106 aa. mbtL, acyl carrier protein, similar to others e.g. ACP_RHIME|P19372 Rhizobium meliloti (77 aa), FASTA scores: opt: 117, E(): 0.03, (29.9% identity in 67 aa overlap) and ACP_SYNY3|P20804 acyl carrier protein (acp) from Synechocystis sp (77 aa), FASTA scores: E(): 7.1e-05, (34.8% identity in 66 aa overlap). Also similar to Rv2244 and Rv0033 from Mycobacterium tuberculosis. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WQF1 No lipid metabolism P63452 P9WQF1 GO:0048037,GO:0005737,GO:0006826,GO:0000036 P9WQF1 MSMEG_2132 Mb1379 NC_000962.3 Mycobrowser_v4 CDS 1438907 1440751 . + 0 Rv1286 cysN ATP sulfurylase may be the GTPase, regulating ATP sulfurylase activity [catalytic activity 1: ATP + sulfate = diphosphate + adenylylsulfate] and APS kinase catalyzes the synthesis of activated sulfate [catalytic activity 2: ATP + adenylylsulfate = ADP + 3'- phosphoadenylylsulfate]. First and second steps in the sulfate activation pathway. These reactions occurs early in the reductive branch of the cysteine biosynthetic pathway. Probable bifunctional enzyme CysN/CysC: sulfate adenyltransferase (subunit 1) + adenylylsulfate kinase Rv1286, (MTCY373.05), len: 614 aa. Probable cysN/cysC bifunctional enzyme, sulfate adenylyltransferase subunit 1 and Adenylylsulfate kinase (see Wooff et al., 2002), similar to CYSN_ECOLI|P23845 sulfate adenylate transferase subunit 1 from Escherichia coli (475 aa), FASTA scores: opt: 1291, E():0, (50.2% identity in 428 aa overlap). Contains 2 x PS00017 ATP/GTP-binding site motif A, PS00301 GTP-binding elongation factors signature. P9WNM5 No intermediary metabolism and respiration Q10600 P9WNM5 GO:0005525,GO:0003924,GO:0004020,GO:0004781,GO:0000103,GO:0005524 2.7.1.25,2.7.7.4 P9WNM5 MMAR_4132 MSMEG_4978 Mb1317 NC_000962.3 Mycobrowser_v4 CDS 1440805 1441290 . + 0 Rv1287 Rv1287 Function unknown Conserved hypothetical protein Rv1287, (MTCY373.06), len: 161 aa. Conserved hypothetical protein, similar to VjeB family of proteins e.g. FASTA score: P44675|Y379_HAEIN hypothetical protein HI0379 (150 aa), FASTA scores: opt: 213, E(): 2.5e-08, (30.0% identity in 130 aa overlap) and YJEB_ECOLI|P21498 hypothetical 15.6 kDa protein in pura-vacb (141 aa), opt: 167, E(): 9.5e-06, (25.0% identity in 136 aa overlap). Belongs to the UPF0074 (RFF2) family. P9WME3 No conserved hypotheticals P67159 P9WME3 GO:0003677 P9WME3 MMAR_4131 MSMEG_4974 Mb1318 NC_000962.3 Mycobrowser_v4 CDS 1441348 1442718 . + 0 Rv1288 Rv1288 Function unknown Conserved protein Rv1288, (MTCY373.07), len: 456 aa. Conserved protein, some similarity to A85B_MYCTU|P31952 antigen 85-b precursor (85b) (325 aa), FASTA scores: opt: 199, E(): 2.7e-06, (24.7% identity in 279 aa overlap). Also similar to Q01377|CSP1_CORGL PS1 protein precursor (related to antigen 85 complex) from Corynebacterium glutamicum (657 aa), FASTA scores: opt: 280, E(): 1.9e-10, (26.4% identity in 352 aa overlap). Seems to contain 3 LYSM repeats P9WM39 No conserved hypotheticals Q10614 P9WM39 GO:0016998 P9WM39 MSMEG_3437 Mb1319 NC_000962.3 Mycobrowser_v4 CDS 1442767 1443399 . + 0 Rv1289 Rv1289 Unknown Unknown protein Rv1289, (MTCY373.08), len: 210 aa. Unknown protein. P9WM37 No conserved hypotheticals P9WM37 P9WM37 MMAR_4123 Mb1320 NC_000962.3 Mycobrowser_v4 CDS 1443482 1445047 . - 0 Rv1290c Rv1290c Function unknown. Thought to be involved in virulence. Conserved protein Rv1290c, (MTCY373.09c), len: 521 aa. Conserved protein (see citation below), similar to AL031013|SC8A6.09 hypothetical protein from Streptomyces coelicolor (443 aa), FASTA scores: opt: 371, E(): 9.5e-17, (28.3% identity in 446 aa overlap). P9WM35 No conserved hypotheticals P0A5E3 P9WM35 GO:0005886,GO:0016021 MMAR_4122 Mb1321c NC_000962.3 Mycobrowser_v4 CDS 1448028 1449371 . + 0 Rv1293 lysA Involved in biosynthesis of lysine (last step) [catalytic activity: MESO-2,6-diaminoheptanedioate = L-lysine + CO(2)]. Diaminopimelate decarboxylase LysA (DAP decarboxylase) Rv1293, (MTCY373.13), len: 447 aa. lysA, diaminopimelate decarboxylase (see citation below), almost identical to DCDA_MYCTU|P31848. Contains PS00878 Orn/DAP/Arg decarboxylases family 2 pyridoxal-P attachment site, PS00879 Orn/DAP/Arg decarboxylases family 2 signature 2. Belongs to family 2 of ornithine, DAP, and arginine decarboxylases. P9WIU7 No intermediary metabolism and respiration 1HKV,1HKW,2O0T P0A5M4 P9WIU7 GO:0009089,GO:0008836 4.1.1.20 P9WIU7 ML1128 MMAR_4104 MSMEG_4958 Mb1325 NC_000962.3 Mycobrowser_v4 CDS 1450697 1451779 . + 0 Rv1295 thrC Involved in threonine biosynthesis [catalytic activity: O-phospho-L-homoserine + H(2)O = L-threonine + phosphate] Threonine synthase ThrC (ts) Rv1295, (MTCY373.15), len: 360 aa. thrC, threonine synthase (see Parish et al., 1999), highly similar to THRC_MYCLE|P45837 Mycobacterium leprae (360 aa), FASTA scores: opt: 2202, E(): 0, (93.9% identity in 359 aa overlap). Contains PS00165 Serine/threonine dehydratases pyridoxal-phosphate attachment site. P9WG59 No intermediary metabolism and respiration 2D1F P66902 P9WG59 GO:0009088,GO:0004795,GO:0030170 4.2.3.1 P9WG59 ML1130 MMAR_4102 MSMEG_4956 Mb1327 NC_000962.3 Mycobrowser_v4 CDS 1451997 1452947 . + 0 Rv1296 thrB Threonine biosynthesis from asparate (fourth step) [catalytic activity: ATP + L-homoserine = ADP + O-phospho-L-homoserine]. Probable homoserine kinase ThrB Rv1296, (MTCY373.16), len: 316 aa. Probable thrB, homoserine kinase (see citation below), highly similar to KHSE_MYCLE|P45836 from Mycobacterium leprae (314 aa), FASTA scores, opt: 1657, E(): 0, (82.0% identity in 311 aa overlap). Contains PS00639 Eukaryotic thiol (cysteine) proteases histidine active site, and PS00627 GHMP kinases putative ATP-binding domain. P9WKE7 No intermediary metabolism and respiration P65224 P9WKE7 GO:0005737,GO:0004413,GO:0016310,GO:0009088,GO:0005524 2.7.1.39 P9WKE7 ML1131 MMAR_4101 MSMEG_4955 Mb1328 NC_000962.3 Mycobrowser_v4 CDS 1455163 1455405 . + 0 Rv1298 rpmE Involved in translation 50S ribosomal protein L31 RpmE Rv1298, (MTCY373.18), len: 80 aa. rpmE, 50s ribosomal protein L31, highly similar to many e.g. RL31_MYCLE|P45834 50s ribosomal protein L31 from Mycobacterium leprae (84 aa), FASTA scores: opt: 490, E(): 5.5e-28, (89.6% identity in 77 aa overlap). Contains PS01143 Ribosomal protein L31 signature. Belongs to the L31P family of ribosomal proteins. P9WHA1 No information pathways P66187 P9WHA1 GO:0019843,GO:0005840,GO:0003735,GO:0006412,GO:0046872 P9WHA1 ML1133 MMAR_4099 MSMEG_4951 Mb1330 NC_000962.3 Mycobrowser_v4 CDS 1455495 1456568 . + 0 Rv1299 prfA Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA Probable peptide chain release factor 1 PrfA (RF-1) Rv1299, (MTCY373.19), len: 357 aa. Probable prfA, peptide chain release factor 1 (rf-1), highly similar to many e.g. RF1_MYCLE|P45833 peptide chain release factor 1 (rf-1) from Mycobacterium leprae (357 aa), FASTA scores: opt: 2047, E(): 0, (89.3% identity in 356 aa overlap); also similar to Mycobacterium tuberculosis Rv3105c, prfB peptide chain release factor 2. Contains PS00745 Prokaryotic-type class I peptide chain release factors signature. Belongs to the prokaryotic and mitochondrial release factors family. P9WHG3 No information pathways P66016 P9WHG3 GO:0016149,GO:0006415,GO:0005737 P9WHG3 ML1134 MMAR_4098 MSMEG_4950 Mb1331 NC_000962.3 Mycobrowser_v4 CDS 2260665 2261144 . + 0 Rv2013 Rv2013 Required for the transposition of an insertion element. Transposase Rv2013, (MTCY39.04c), len: 159 aa. Transposase, shows similarity to N-terminal part of transposase and insertion element hypothetical proteins. Length changed since first submission (no clear start apparent). Q10844 No insertion seqs and phages Q10844 Q10844 GO:0004803,GO:0006313,GO:0003677 Q10844 Mb2036 NC_000962.3 Mycobrowser_v4 CDS 1459766 1460251 . + 0 Rv1303 Rv1303 Function unknown Conserved hypothetical transmembrane protein Rv1303, (MTCY373.23), len: 161 aa. Conserved hypothetical transmembrane protein, highly similar to P53431|Y02N_MYCLE hypothetical Mycobacterium leprae protein (153 aa), FASTA score: opt: 636, E():0, (69.8% identity in 149 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WM31 No cell wall and cell processes P64801 P9WM31 GO:0015078,GO:0016021,GO:0005886,GO:0045263,GO:0015986 P9WM31 ML1138 MMAR_4094 MSMEG_4943 Mb1335 NC_000962.3 Mycobrowser_v4 CDS 1460244 1460996 . + 0 Rv1304 atpB Key component of the proton channel; it may play a direct role in the translocation of protons (H+) across the membrane Probable ATP synthase a chain AtpB (protein 6) Rv1304, (MTCY373.24), len: 250 aa. Probable atpB, ATP synthase a chain, highly similar to ATP6_MYCLE|P45829 Mycobacterium leprae (251 aa), FASTA scores: opt: 1382, E(): 0, (84.0% identity in 250 aa overlap). Contains PS00449 ATP synthase a subunit signature. subunit: F-type ATPases have 2 components, cf(1) - the catalytic core - and cf(0) - the membrane proton channel. cf(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). cf(0) has three main subunits: A, B and C. Belongs to the ATPase a chain family. P9WPV7 No intermediary metabolism and respiration P63654 P9WPV7 GO:0016021,GO:0005886,GO:0042777,GO:0045263,GO:0046933 P9WPV7 ML1139 MMAR_4093 MSMEG_4942 Mb1336 NC_000962.3 Mycobrowser_v4 CDS 1461045 1461290 . + 0 Rv1305 atpE This is one of the three chains of the nonenzymatic component (cf(0) subunit) of the ATPase complex. Probable ATP synthase C chain AtpE (lipid-binding protein) (dicyclohexylcarbodiimide-binding protein) Rv1305, (MTCY373.25), len: 81 aa. Probable atpE, ATP synthase C chain, highly similar to P45828|ATPL_MYCLE Mycobacterium leprae (92.6% identity in 81 aa overlap). Contains PS00605 ATP synthase C subunit signature. subunit: F-type ATPases have 2 components, cf(1) - the catalytic core - and cf(0) - the membrane proton channel. cf(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). cf(0) has three main subunits: A, B and C. Belongs to the ATPase C chain family. P9WPS1 No intermediary metabolism and respiration P63691 P9WPS1 GO:0046933,GO:0016021,GO:0008289,GO:0005886,GO:0045263,GO:0015986 P9WPS1 ML1140 MMAR_4092 MSMEG_4941 Mb1337 NC_000962.3 Mycobrowser_v4 CDS 1461321 1461836 . + 0 Rv1306 atpF This is one of the three chains of the nonenzymatic component (cf(0) subunit) of the ATPase complex. Probable ATP synthase B chain AtpF Rv1306, (MTCY373.26), len: 171 aa. Probable atpF, ATP synthase B chain, highly similar to ATPF_MYCLE P45827 (170 aa), FASTA scores, opt: 802, E(): 0, (79.5% identity in 171 aa overlap). subunit: F-type ATPases have 2 components, cf(1) - the catalytic core - and cf(0) - the membrane proton channel. cf(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). cf(0) has three main subunits: A, B and C. Belongs to the ATPase B chain family. P9WPV5 No intermediary metabolism and respiration P63656 P9WPV5 GO:0046933,GO:0016021,GO:0005886,GO:0045263,GO:0015986 P9WPV5 ML1141 MMAR_4091 MSMEG_4940 Mb1338 NC_000962.3 Mycobrowser_v4 CDS 1470321 1471577 . + 0 Rv1315 murA Involved in cell wall formation; peptidoglycan biosynthesis. Adds enolpyruvyl to UDP-N-acetylglucosamine [catalytic activity: phosphoenolpyruvate + UDP-N-acetyl-D- glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine] Probable UDP-N-acetylglucosamine 1-carboxyvinyltransferase MurA Rv1315, (MTCY373.35-MTCY149.01), len: 418 aa. Probable murA, UDP-N-acetylglucosamine 1-carboxyvinyltransferase (see Belanger & Inamine 2000), highly similar to many e.g. MURA_MYCLE|P45821 (418 aa), FASTA scores: opt: 2495, E(): 0, (96.2% identity in 396 aa overlap). Belongs to the EPSP synthase family. MURA subfamily. P9WJM1 No cell wall and cell processes P0A5L2 P9WJM1 GO:0008760,GO:0007049,GO:0051301,GO:0007047,GO:0005737,GO:0009252,GO:0008360,GO:0019277 2.5.1.7 P9WJM1 ML1150 MMAR_4083 MSMEG_4932 Mb1348 NC_000962.3 Mycobrowser_v4 CDS 1467315 1467680 . + 0 Rv1311 atpC Produces ATP from ADP in the presence of a proton gradient across the membrane [catalytic activity: ATP + H(2)O + H(+)(in) = ADP + phosphate + H(+)(out)] Probable ATP synthase epsilon chain AtpC Rv1311, (MTCY373.31), len: 121 aa. Probable atpC, ATP synthase epsilon chain, highly similar to ATPE_MYCLE|P45822 Mycobacterium leprae (124 aa), FASTA scores: opt: 682, E(): 5.4e-40, (87.6% identity in 121 aa overlap). subunit: F-type ATPases have 2 components, cf(1) - the catalytic core - and cf(0) - the membrane proton channel. cf(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). cf(0) has three main subunits: A, B and C. Belongs to the ATPase epsilon chain family. P9WPV1 No intermediary metabolism and respiration P63662 P9WPV1 GO:0046933,GO:0005886,GO:0042777,GO:0045261,GO:0046961,GO:0005524 P9WPV1 ML1146 MMAR_4086 MSMEG_4935 Mb1343 NC_000962.3 Mycobrowser_v4 CDS 1464884 1465801 . + 0 Rv1309 atpG Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the cf(0) complex. Probable ATP synthase gamma chain AtpG Rv1309, (MTCY373.29), len: 305 aa. Probable atpG, ATP synthase gamma chain, highly similar to ATPG_MYCLE|P45824 ATP synthase gamma chain from Mycobacterium leprae (298 aa), FASTA scores: opt: 1579, E():0, (83.9% identity in 305 aa overlap). Contains PS00153 ATP synthase gamma subunit signature. subunit: F-type ATPases have 2 components, cf(1) - the catalytic core - and cf(0) - the membrane proton channel. cf(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). cf(0) has three main subunits: A, B and C. Belongs to the ATPase gamma chain family. P9WPU9 No intermediary metabolism and respiration P63671 P9WPU9 GO:0015986,GO:0046933,GO:0005886,GO:0045261,GO:0046961,GO:0005524 P9WPU9 ML1144 MMAR_4088 MSMEG_4937 Mb1341 NC_000962.3 Mycobrowser_v4 CDS 1465841 1467301 . + 0 Rv1310 atpD Produces ATP from ADP in the presence of a proton gradient across the membrane. The beta chain is the catalytic subunit [catalytic activity: ATP + H(2)O + H(+)(in) = ADP + phosphate + H(+)(out)] Probable ATP synthase beta chain AtpD Rv1310, (MTCY373.30), len: 486 aa. Probable atpD, ATP synthase beta chain, highly similar to ATPB_MYCLE|P45823 Mycobacterium leprae (485 aa), FASTA score: opt: 2916, E(): 0, (92.6% identity in 484 aa overlap). Contains PS00017 ATP/GTP-binding site motif A, PS00152 ATP synthase alpha and beta subunits signature. subunit: F-type ATPases have 2 components, cf(1) - the catalytic core - and cf(0) - the membrane proton channel. cf(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). cf(0) has three main subunits: A, B and C. Belongs to the ATPase alpha/beta chains family. P9WPU5 No intermediary metabolism and respiration P63677 P9WPU5 GO:0046933,GO:0008553,GO:0005886,GO:0042777,GO:0045261,GO:0046961,GO:0005524 3.6.3.14 P9WPU5 ML1145 MMAR_4087 MSMEG_4936 Mb1342 NC_000962.3 Mycobrowser_v4 CDS 1467688 1468131 . + 0 Rv1312 Rv1312 Unknown Conserved hypothetical secreted protein Rv1312, (MTCY373.32), len: 147 aa. Conserved hypothetical secreted protein with potential N-terminal signal sequence. Highly similar to P53432|Y02W_MYCLE hypothetical Mycobacterium leprae protein (147 aa), FASTA score: opt: 884, E(): 0, (88.4% identity in 147 aa overlap). N-terminus hydrophobic. P9WM29 No cell wall and cell processes Q10620 P9WM29 GO:0016021 ML1147 MMAR_4085 MSMEG_4933 Mb1344 NC_000962.3 Mycobrowser_v4 CDS 1500661 1500966 . + 0 Rv1331 Rv1331 Function unknown Conserved hypothetical protein Rv1331, (MTCY130.16), len: 101 aa. Conserved hypothetical protein, highly similar to U00014|ML014 B1549_C2_207 from Mycobacterium leprae (94 aa), FASTA scores: opt: 573, E(): 2.9e-40, (90.3% identity in 93 aa overlap). Similar to AL096852|SCE19A_16 hypothetical protein from Streptomyces coelicolor (105 aa), FASTA scores: opt: 377, E(): 2.9e-22, (60.0% identity in 105 aa overlap). P9WPC1 No conserved hypotheticals P67647 P9WPC1 GO:0006508,GO:0030163 P9WPC1 ML1165 MMAR_4067 MSMEG_4910 Mb1366 NC_000962.3 Mycobrowser_v4 CDS 1508184 1508546 . - 0 Rv1342c Rv1342c Unknown Conserved membrane protein Rv1342c, (MTCY02B10.06c), len: 120 aa. Conserved membrane protein. Highly similar to G466926|P54133 hypothetical protein B1549_F2_59 from Mycobacterium leprae (119 aa), FASTA scores, opt: 544, E(): 1.9e-29, (68.3 % identity in 120 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WM19 No cell wall and cell processes P9WM19 GO:0005886,GO:0016021 ML1176,ML1176c MMAR_4039 MSMEG_4900 Mb1377c NC_000962.3 Mycobrowser_v4 CDS 2341808 2342944 . + 0 Rv2084 Rv2084 Unknown Hypothetical protein Rv2084, (MTCY49.23), len: 378 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLK1 No unknown P9WLK1 P9WLK1 Mb2110,Mb2111 NC_000962.3 Mycobrowser_v4 CDS 1469671 1470252 . - 0 Rv1314c Rv1314c Function unknown Conserved protein Rv1314c, (MTCY373.34c), len: 193 aa. Conserved protein, highly similar to P53523|Y02Y_MYCLE hypothetical Mycobacterium leprae protein (191 aa), FASTA score: opt:1019, E(): 0, (81.2% identity in 191 aa overlap). Some similarity with YDHW_CITFR|P45515 hypothetical 19.8 kDa protein in dhar-dhat intergenic region (176 aa), FASTA scores: opt: 297, E(): 1.6e-13, (37.6% identity in 178 aa overlap). Also similar to hypothetical protein AE002007|AE002007_3 Deinococcus radiodurans (185 aa), FASTA score: opt: 386, E(): 7.7e-19, (42.4% identity in 172 aa overlap). P9WP99 No conserved hypotheticals 2G2D P64803 P9WP99 GO:0008817,GO:0009236,GO:0005737,GO:0005524 2.5.1.17 P9WP99 ML1149,ML1149c MMAR_4084 MSMEG_4934 Mb1347c NC_000962.3 Mycobrowser_v4 CDS 1477134 1477631 . - 0 Rv1316c ogt Repair of alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated [catalytic activity: DNA (containing 6-O-methylguanine) + [protein]-L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.] Methylated-DNA--protein-cysteine methyltransferase Ogt (6-O-methylguanine-DNA methyltransferase) (O-6-methylguanine-DNA-alkyltransferase) Rv1316c, (MTCY130.01c), len: 165 aa. Ogt, methylated-dna--protein-cysteine methytransferase (see citation below), similar to many e.g. OGT_HAEIN|P44687 Haemophilus influenzae (190 aa), FASTA scores: opt: 405, E(): 6.5e-20, (41.9% identity in 155 aa overlap). Contains PS00374 Methylated-DNA--protein-cysteine methyltransferase active site. P9WJW5 No information pathways P0A696 P9WJW5 GO:0003908,GO:0006281 2.1.1.63 P9WJW5 ML1151,ML1151c MMAR_4082 MSMEG_4928 Mb1349c NC_000962.3 Mycobrowser_v4 CDS 1479199 1480824 . - 0 Rv1318c Rv1318c Thought to play an essential role in regulation of cellular metabolism by catalysing the synthesis of a second messenger, camp [catalytic activity: ATP = 3',5'-cyclic AMP + pyrophosphate]. Possible adenylate cyclase (ATP pyrophosphate-lyase) (adenylyl cyclase) Rv1318c, (MTCY130.03c), len: 541 aa. Possible adenylate cyclase. Some similarity at the c-terminus to CYAA_RHIME|P19485 adenylate cyclase from Rhizobium meliloti (193 aa), FASTA scores, opt: 270, E(): 2.5e-11, (28.8% identity in 184 aa overlap); similar to other mycbacterium tuberculosis putative adenylate cyclases e.g. Rv1319c|MTCY130.04c (535 aa), FASTA scores: opt: 2505, E(): 0, (71.0% identity in 534 aa overlap), also similar to Rv1320c|MTCY130.05c (567 aa), FASTA scores, opt: 2423, E(): 0, (68.7% identity in 534 aa overlap). N-terminus is hydrophobic. Belongs to adenylyl cyclase class-3 family. P9WQ33 No intermediary metabolism and respiration P63527 P9WQ33 GO:0016021,GO:0016849,GO:0005886,GO:0004871,GO:0007165,GO:0009190 P9WQ33 ML1154,ML1154c MMAR_4078 MSMEG_4924 Mb1352c NC_000962.3 Mycobrowser_v4 CDS 1500926 1501582 . + 0 Rv1332 Rv1332 Involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv1332, (MTCY130.17), len: 218 aa. Possible regulatory protein, high similarity to ML014|U00014 M. leprae B1549_C3_236 (222 aa), FASTA scores: opt: 1158, E(): 0, (75.6% identity in 221 aa overlap). Helix turn helix motif fram aa 8-29 (+3.03 SD). P9WM25 No regulatory proteins P64809 P9WM25 ML1166 MMAR_4066 MSMEG_4909 Mb1367 NC_000962.3 Mycobrowser_v4 CDS 1482514 1484217 . - 0 Rv1320c Rv1320c Thought to play an essential role in regulation of cellular metabolism by catalysing the synthesis of a second messenger, cAMP. May be involved in virulence [catalytic activity: ATP = 3',5'-cyclic AMP + pyrophosphate]. Possible adenylate cyclase (ATP pyrophosphate-lyase) (adenylyl cyclase) Rv1320c, (MTCY130.05c), len: 567 aa. Possible adenylate cyclase (see Rindi et al., 1999). Some similarity at the C-terminus to CYAA_RHIME|P19485 adenylate cyclase from Rhizobium meliloti (193 aa), FASTA scores: opt: 277, E(): 2e-12, (34.0% identity in 156 aa overlap); similar to other mycbacterium tuberculosis putative adenylate cyclases e.g. Rv1318c|MTCY130.03c (541 aa), FASTA scores: opt: 2423, E(): 0, (68.7% identity in 534 aa overlap); Rv1319c|MTCY130.04c (535 aa), FASTA scores: opt: 2354, E(): 0, (66.3% identity in 534 aa overlap). N-terminus is hydrophobic. Belongs to adenylyl cyclase class-3 family. P9WQ29 No intermediary metabolism and respiration Q10633 P9WQ29 GO:0016021,GO:0016849,GO:0005886,GO:0004871,GO:0007165,GO:0009190 P9WQ29 MMAR_4079 Mb1354c NC_000962.3 Mycobrowser_v4 CDS 1485862 1487031 . + 0 Rv1323 fadA4 Function unknown, but supposed involvement in lipid degradation [catalytic activity: 2 acetyl-CoA = CoA + acetoacetyl-CoA]. Probable acetyl-CoA acetyltransferase FadA4 (acetoacetyl-CoA thiolase) Rv1323, (MTCY130.08), len: 389 aa. Probable fadA4, acetyl-CoA acetyltransferase, equivalent to THIL_MYCLE|P46707 possible acetyl-CoA C-acetyltransferase from Mycobacterium leprae (393 aa), FASTA scores: opt: 2218, E(): 0, (87.0% identity in 392 aa overlap). Also highly similar to others e.g. CAB70629.1|AL137242 probable acetoacetyl-CoA thiolase from Streptomyces coelicolor (401 aa); T51772 acetyl-CoA C-acetyltransferase [validated] from Alcaligenes latus (392 aa); etc. Some homologies indicate ATA start codon. Contains PS00098 Thiolases acyl-enzyme intermediate signature, PS00737 Thiolases signature 2, and PS00099 Thiolases active site. Belongs to the thiolase family. P9WG69 No lipid metabolism P66926 P9WG69 GO:0008152,GO:0003985 2.3.1.9 P9WG69 ML2162,ML2162c MMAR_4075 MSMEG_4920 Mb1358 NC_000962.3 Mycobrowser_v4 CDS 1487161 1488075 . + 0 Rv1324 Rv1324 Thioredoxin participates in various redox reactions through the reversible oxidation of its active center dithiol, to a disulfide, & catalyzes dithiol-disulfide exchange reactions Possible thioredoxin Rv1324, (MTCY130.09), len: 304 aa. Possible thioredoxin, similar to several e.g. U00014|Q49716 TRXA from Mycobacterium leprae (255 aa), FASTA scores: opt: 1014, E(): 0, (69.7% identity in 228 aa overlap); THIO_RHOSH|P08058 TrxA from Rhodobacter sphaeroides (105 aa), FASTA scores: opt 196, E(): 1.9e-06, (33.0% identity in 103 aa overlap). Contains PS00339 Aminoacyl-transfer RNA synthetases class-II signature 2. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WG61 No intermediary metabolism and respiration P64807 P9WG61 GO:0045454 P9WG61 ML1159 MMAR_4074 MSMEG_4917 Mb1359 NC_000962.3 Mycobrowser_v4 CDS 1518231 1518560 . + 0 Rv1351 Rv1351 Unknown Hypothetical protein Rv1351, (MTCY02B10.15), len: 109 aa. Hypothetical unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WM17 No conserved hypotheticals P9WM17 Mb1386 NC_000962.3 Mycobrowser_v4 CDS 1490117 1492312 . - 0 Rv1326c glgB Involved in glycogen biosynthesis (cytoplasmic polysaccharides) (third step) [catalytic activity: formation of 1,6-glucosidic linkages of glycogen]. 1,4-alpha-glucan branching enzyme GlgB (glycogen branching enzyme) Rv1326c, (MTCY130.11c), len: 731 aa. glgB, 1,4-alpha-glucan branching enzyme, similar to others e.g. GLGB_ECOLI|P07762 Escherichia coli (728 aa), FASTA scores: opt: 2330, E(): 0, (48.7% identity in 719 aa overlap). Similar to other Mycobacterium tuberculosis putative alpha-glucan branching enzymes Rv1562c, Rv1563c. Belongs to family 13 of glycosyl hydrolases, also known as the alpha-amylase family. P9WN45 No intermediary metabolism and respiration 3K1D Q10625 P9WN45 GO:0043169,GO:0005978,GO:0004553,GO:0003844 2.4.1.18 P9WN45 ML1160,ML1160c MMAR_4072 MSMEG_4918 Mb1361c NC_000962.3 Mycobrowser_v4 CDS 1494564 1497155 . + 0 Rv1328 glgP Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties [catalytic activity: {(1,4)-alpha-D-glucosyl}(N) + phosphate = {(1,4)-alpha-D-glucosyl}(N-1) + alpha-D-glucose 1-phosphate]. Probable glycogen phosphorylase GlgP Rv1328, (MTCY130.13), len: 863 aa. Probable glgP, glycogen phosphorylase, similar to many e.g. PHSG_HAEIN|P45180 glycogen phosphorylase from Haemophilus influenzae (821 aa), FASTA scores: E(): 6.9e-08, (25.6% identity in 675 aa overlap). Belongs to the glycogen phosphorylase family. P9WMW1 No intermediary metabolism and respiration Q10639 P9WMW1 GO:0004645,GO:0030170,GO:0005977 2.4.1.1 P9WMW1 ML1162 MMAR_4070 MSMEG_4915 Mb1363 NC_000962.3 Mycobrowser_v4 CDS 1497195 1499189 . - 0 Rv1329c dinG Probable helicase involved in DNA repair and perhaps also replication. Probable ATP-dependent helicase DinG Rv1329c, (MTCY130.14c), len: 664 aa. Probable dinG, ATP-dependent helicase (see citation below), similar to several e.g. DING_HAEIN|P44680 probable ATP-dependent helicase ding from Haemophilus influenzae (640 aa), FASTA scores: opt: 685, E(): 2.3e-38, (32.8% identity in 644 aa overlap). Contains PS00017 ATP/GTP-binding site motif A. P9WMR5 No information pathways P64314 P9WMR5 GO:0008026,GO:0003677,GO:0006310,GO:0006281,GO:0005524 3.6.4.12 P9WMR5 MMAR_4069 MSMEG_4912 Mb1364c NC_000962.3 Mycobrowser_v4 CDS 1503394 1504365 . + 0 Rv1336 cysM Involved in cysteine biosynthesis [catalytic activity: O-phospho-L-serine + H(2)S = L-cysteine + phosphate] Cysteine synthase B CysM (CSASE B) (O-phosphoserine sulfhydrylase B) (O-phosphoserine (thiol)-lyase B) Rv1336, (MTCY130.21), len: 323 aa. cysM, cysteine synthase B, similar to many e.g. CYSM_ECOLI|P16703 Escherichia coli (303 aa), FASTA scores: opt: 720, E(): 4.6e-40, (41.1% identity in 302 aa overlap). Also similar to other Mycobacterium tuberculosis cysteine synthase subunits e.g. Rv1077, Rv2334, Rv0848, etc. Contains PS00901 Cysteine synthase/cystathionine beta-synthase P-phosphate attachment site. Belongs to the cysteine synthase/cystathionine beta-synthase family. P9WP53 No intermediary metabolism and respiration 3DKI,3DWG,3DWI,3FGP P63873 P9WP53 GO:0004124,GO:0030170,GO:0016740,GO:0006535 2.5.1.47 P9WP53 ML1170 MMAR_4629 MSMEG_4905 Mb1371 NC_000962.3 Mycobrowser_v4 CDS 1518763 1519134 . + 0 Rv1352 Rv1352 Function unknown Conserved protein Rv1352, (MTCY02B10.16), len: 123 aa. Conserved protein, some similarity to Rv1906c|MTCY180.12 hypothetical protein from Mycobacterium tuberculosis (156 aa), FASTA scores: E(): 4e-05, (36.2% identity in 116 aa overlap). P9WM15 No conserved hypotheticals P9WM15 P9WM15 MMAR_4028 Mb1387 NC_000962.3 Mycobrowser_v4 CDS 2358033 2358206 . - 0 Rv2099c PE21 Function unknown PE family protein PE21 Rv2099c, (MTCY49.39c), len: 58 aa. PE21, Member of the Mycobacterium tuberculosis PE family (see Brennan and Delogu, 2002); 5'-end of Rv2098c|PE_PGRS36|MTCY49.38c, then frameshifts. Sequence has been checked, no errors found. Q79FH7 Yes PE/PPE Q79FH7 Q79FH7 Mb2125c NC_000962.3 Mycobrowser_v4 CDS 1501599 1502633 . + 0 Rv1333 Rv1333 Function unknown; probably involved in cellular metabolism Probable hydrolase Rv1333, (MTCY130.18), len: 344 aa. Possible hydrolase, similar to Q57326|D26094 endo-type 6-aminohexanoate oligomer hydrolase (355 aa), fasta scores: E(): 1.4e-10, (31.9% identity in 339 aa overlap). Equivalent to P53425|YD33_MYCLE hypothetical 36.1 KD protein B154 Mycobacterium leprae (362 aa), FASTA scores: opt: 1735, E(): 0, (76.7% identity in 352 aa overlap). P9WM23 No intermediary metabolism and respiration P64811 P9WM23 GO:0005886,GO:0016021 P9WM23 ML1167 MMAR_4065 MSMEG_4908 Mb1368 NC_000962.3 Mycobrowser_v4 CDS 1502641 1503081 . + 0 Rv1334 mec Possibly involved in cysteine biosynthesis. Generates cysteine from hydrolysis of CysO-CYS. Possible hydrolase Rv1334, (MTCY130.19), len: 146 aa. Possible mec, hydrolase (See Burns et al., 2005), similar to AL096852|SCE19A_13 hypothetical protein from Streptomyces coelicolor (140 aa), Fasta scores: opt: 579, E(): 0, (65.0% identity in 140 aa overlap); and Q54330|M29166 MEC+ from Streptomyces kasugaensis (115 aa), FASTA scores; E(): 7.6e-33, (56.9% identity in 109 aa overlap). P9WHS1 No intermediary metabolism and respiration P64813 P9WHS1 P9WHS1 ML1168 MMAR_4064 MSMEG_4907 Mb1369 NC_000962.3 Mycobrowser_v4 CDS 1503103 1503384 . + 0 Rv1335 cysO Probably involved in cysteine biosynthesis, as sulfur donor in thiocarboxylated form. Sulfur carrier protein CysO Rv1335, (MT1376.1, MTCY130.20), len: 93 aa. CysO, sulfur carrier protein (See Burns et al., 2005). Note that previously known as cfp10A. Similar to hypothetical proteins from other organisms e.g. P74060|D90911 Synechocystis (109 aa), FASTA scores: E(): 2.3e-20, (49.5% identity in 93 aa overlap). P9WP33 No intermediary metabolism and respiration 3DWG,3DWM P0A646 P9WP33 GO:0006790 P9WP33 ML1169 MMAR_4063 MSMEG_4906 Mb1370 NC_000962.3 Mycobrowser_v4 CDS 1505075 1505890 . + 0 Rv1338 murI Involved in peptidoglycan biosynthesis. Provides the (R)-glutamic acid required for cell wall biosynthesis [catalytic activity: L-glutamate = D-glutamate] Probable glutamate racemase MurI Rv1338, (MTCY130.23), len: 271 aa. Probable murI, glutamate racemase, highly similar to many e.g. MURI_MYCLE|P46705 (272 aa), FASTA scores: opt: 1559, E(): 0, (88.9% identity in 271 aa overlap). Contains PS00924 Aspartate and glutamate racemases signature 2. P9WPW9 No cell wall and cell processes P63635 P9WPW9 GO:0008881,GO:0009252,GO:0008360,GO:0007047 5.1.1.3 P9WPW9 ML1172 MMAR_4058 MSMEG_4903 Mb1373 NC_000962.3 Mycobrowser_v4 CDS 1505917 1506738 . + 0 Rv1339 Rv1339 Function unknown Conserved protein Rv1339, (MTCY130.24), len: 273 aa. Conserved protein, highly similar to Y211_MYCLE|P50474 hypothetical protein b1549_c2_211 from Mycobacterium leprae (284 aa), FASTA scores: opt: 1672, E(): 0, (86.2% identity in 276 aa overlap). Also similar to AL096852|SCE19A.08 hypothetical protein from Streptomyces coelicolor (250 aa), FASTA scores: opt: 630, E(): 0, (42.2% identity in 256 aa overlap). Similar to M. tuberculosis hypothetical proteins Rv3796, Rv2407. Predicted to be an outer membrane protein (See Song et al., 2008). P9WGC1 No conserved hypotheticals P9WGC1 GO:0016787 P9WGC1 ML1173 MMAR_4043 MSMEG_4902 Mb1374 NC_000962.3 Mycobrowser_v4 CDS 1506755 1507534 . + 0 Rv1340 rphA RNase PH is a phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [catalytic activity: {tRNA}(N+1) + phosphate = {tRNA}(N) + a nucleoside diphosphate]. Probable ribonuclease RphA (RNase PH) (tRNA nucleotidyltransferase) Rv1340, (MTCY130.25), len: 259 aa. Probable rphA, Ribonuclease ph, highly similar to others e.g. RNPH_MYCLE|P37939 Mycobacterium leprae (259 aa), FASTA scores: opt: 1524, E(): 0, (88.8% identity in 259 aa overlap). Belongs to the RNASE PH family. P9WGZ7 No information pathways 3B4T Q10628 P9WGZ7 GO:0000049,GO:0009022,GO:0008033,GO:0004549,GO:0000175 2.7.7.56 P9WGZ7 ML1174 MMAR_4042 MSMEG_4901 Mb1375 NC_000962.3 Mycobrowser_v4 CDS 1507573 1508187 . + 0 Rv1341 Rv1341 Function unknown Conserved protein Rv1341, (MTCY130.26), len: 204 aa. Conserved protein, some similarity to P52061|YGGV_ECOLI hypothetical protein yggV (197 aa), FASTA scores: opt: 521, E(): 7.9e-27, (46.0% identity in 200 aa overlap). Equivalent to ML014|U00014 hypothetical protein B1549_C2_213 from Mycobacterium leprae (285 aa), FASTA scores: opt: 1073, E(): 0, (83.0% identity in 206 aa overlap). P9WMR7 No conserved hypotheticals P64307 P9WMR7 GO:0017111 3.6.1.15 P9WMR7 ML1175 MMAR_4040 MSMEG_4899 Mb1376 NC_000962.3 Mycobrowser_v4 CDS 1510846 1512006 . + 0 Rv1346 mbtN Thought to be involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins Acyl-CoA dehydrogenase MbtN Rv1346, (MTCY02B10.10), len: 386 aa. mbtN, acyl-CoA dehydrogenase, similar to many e.g. NP_251579.1|NC_002516 probable acyl-CoA dehydrogenase from Pseudomonas aeruginosa (386 aa); NP_036951.1|NM_012819|ACDL_RAT|P15650 acyl Coenzyme A dehydrogenase (long chain) from Rattus norvegicus (430 aa), FASTA scores: opt: 414, E(): 1.2e-18, (26.1% identity in 376 aa overlap); etc. Note that previously known as fadE14. P9WQF9 No lipid metabolism P63431 P9WQF9 GO:0006826,GO:0055114,GO:0003995 1.3.99.- P9WQF9 MSMEG_2130 Mb1381 NC_000962.3 Mycobrowser_v4 CDS 1511973 1512605 . - 0 Rv1347c mbtK Involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins Lysine N-acetyltransferase MbtK Rv1347c, (MTCY02B10.11c), len: 210 aa. MbtK, lysine N-acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Some similarity to the C-terminus of malonyl-coenzyme A carboxylases e.g. G545170 malonyl-coenzyme A carboxylase (417 aa), FASTA scores: opt: 392, E(): 4.9 e-20, (35.6% identity in 174 aa overlap). P9WK15 No lipid metabolism 1YK3 P64819 P9WK15 GO:0006826,GO:0008415 2.3.1.- P9WK15 MMAR_1587 MSMEG_2128 Mb1382c NC_000962.3 Mycobrowser_v4 CDS 1531348 1532370 . + 0 Rv1360 Rv1360 Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv1360, (MTCY02B10.24), len: 340 aa. Probable oxidoreductase. Similar to Q49598|G1002714 coenzyme F420-dependent n5, n10-methylenetetrahydromethanopterin reductase from Methanopyrus kandleri (349 aa), FASTA scores: opt: 264, E(): 4.4e-11, (26.3% identity in 323 aa overlap). P9WM03 No intermediary metabolism and respiration P64831 P9WM03 P9WM03 MSMEG_2256 Mb1395 NC_000962.3 Mycobrowser_v4 CDS 1539512 1540645 . - 0 Rv1367c Rv1367c Function unknown (possibly involved in cell wall biosynthesis). Conserved protein Rv1367c, (MTCY02B12.01c,MTCY02B10.31c), len: 377 aa. Conserved protein. Some similarity to penicillin binding proteins e.g. PBPE_BACSU|P32959 penicillin-binding protein 4* (pbp 4*) from Bacillus subtilis (451 aa), FASTA scores: E(): 6.9e-06, (23.6% identity in 373 aa overlap). Similar to AL031107|SC5A7.06 hypothetical protein from Streptomyces coelicolor (409 aa), FASTA scores: opt: 675, E(): 0, (40.4% identity in 339 aa overlap). P9WLZ3 No conserved hypotheticals Q11037 P9WLZ3 P9WLZ3 ML0528,ML0528c MMAR_2187 MSMEG_3040 Mb1402c NC_000962.3 Mycobrowser_v4 CDS 1541994 1542980 . - 0 Rv1369c Rv1369c Possibly required for the transposition of the insertion element IS6110. Probable transposase Rv1369c, (MTCY02B12.03c), len: 328 aa. Probable transposase subunit for IS6110. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv1368c and Rv1369c, the sequence UUUUAAAG (directly upstream of Rv1369c) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 34 aa). P9WKH9 No insertion seqs and phages P0C5G8 P9WKH9 GO:0015074,GO:0006310,GO:0032196,GO:0003677 P9WKH9 NC_000962.3 Mycobrowser_v4 CDS 1517491 1518234 . + 0 Rv1350 fabG2 Involved in the fatty acid biosynthesis pathway (first reduction step) [catalytic activity: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH]. Probable 3-oxoacyl-[acyl-carrier protein] reductase FabG2 (3-ketoacyl-acyl carrier protein reductase) Rv1350, (MTCY02B10.14), len: 247 aa. Probable fabG2, 3-oxoacyl-[acyl-carrier protein] reductase, highly similar to many e.g. NP_350157.1|NC_003030 3-ketoacyl-acyl carrier protein reductase from Clostridium acetobutylicum (249 aa); NP_229523.1|NC_000853 3-oxoacyl-(acyl carrier protein) reductase from Thermotoga maritima (246 aa); AAC44307.1|U59433 3-ketoacyl-acyl carrier protein reductase from Bacillus subtilis (246 aa); etc. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. P9WGR9 No lipid metabolism P66781 P9WGR9 GO:0055114,GO:0016491 1.-.-.- P9WGR9 ML1178 MMAR_4033 MSMEG_2206 Mb1385 NC_000962.3 Mycobrowser_v4 CDS 1519200 1519985 . - 0 Rv1353c Rv1353c Involved in transcriptional mechanism Probable transcriptional regulatory protein Rv1353c, (MTCY02B10.17c), len: 261 aa. Probable transcriptional regulatory protein, similar to TER1_ECOLI|P03038 tetracycline repressor protein class a from Escherichia coli (216 aa), FASTA scores, opt: 231, E(): 1.6e-08, (31.3% identity in 211 aa overlap). Helix turn helix motif present at aa 3859 (+3.59 SD). Belongs to the TetR/AcrR family of transcriptional regulators. P9WMD3 No regulatory proteins P67434 P9WMD3 GO:0006355,GO:0016566,GO:0006350,GO:0003700,GO:0016481 P9WMD3 MMAR_4019 MSMEG_2225 Mb1388c NC_000962.3 Mycobrowser_v4 CDS 1521885 1524032 . - 0 Rv1355c moeY Involved in biosynthesis of a demolybdo cofactor (molybdopterin), necessary for molybdoenzymes. Plays a role in the activation of the small subunit of the molybdopterin converting factor (MOAD) Possible molybdopterin biosynthesis protein MoeY Rv1355c, (MTCY02B10.19c), len: 715 aa. Possible moeY, Molybdopterin biosynthesis protein, very weak similarity to MOEB_ECOLI|P12282 molybdopterin biosynthesis moeb protein (249 aa), FASTA scores, opt: 180, E(): 8.5e-05, (29.3% identity in 174 aa overlap). P9WM11 No intermediary metabolism and respiration Q11025 P9WM11 GO:0003824,GO:0008152 P9WM11 ML1751,ML1751c MMAR_3826,MMAR_4921 MSMEG_2198 Mb1390c NC_000962.3 Mycobrowser_v4 CDS 1524029 1524820 . - 0 Rv1356c Rv1356c Unknown Hypothetical protein Rv1356c, (MTCY02B10.20c), len: 263 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WM09 No conserved hypotheticals P9WM09 MSMEG_2199 Mb1391c NC_000962.3 Mycobrowser_v4 CDS 1525293 1526216 . - 0 Rv1357c Rv1357c Function unknown Conserved hypothetical protein Rv1357c, (MTCY02B10.21c), len: 307 aa. Conserved hypothetical protein, similar to members of the YEGE/YHJK/YJCC family e.g. Y4LL_RHISN|P55552 hypothetical protein Y4ll from Rhizobium sp. (827 aa), FASTA scores: E(): 0, (37.7% identity in 257 aa overlap), also similar to Rv1354c|MTCY02B10.18c (34.0% identity in 253 aa overlap). Belongs to the YEGE/YHDA/YHJK/YJCC family. P9WM07 No conserved hypotheticals P64829 P9WM07 GO:0005886,GO:0016021 P9WM07 ML1752,ML1752c Mb1392c NC_000962.3 Mycobrowser_v4 CDS 1532443 1533633 . - 0 Rv1361c PPE19 Function unknown PPE family protein PPE19 Rv1361c, (MTCY02B10.25c), len: 396 aa. PPE19 (alternate gene name: mtb39b). Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, highly similar to many e.g. Rv1196|MTCI364.08|PPE18, FASTA scores: E(): 0, (84.9% identity in 397 aa overlap); MTCY274.23c (42.3% identity in 416 aa overlap); etc. Contains PS00501 Signal peptidases I serine active site. Note that expression of Rv1361c was demonstrated in lysates by immunodetection (see Dillon et al., 1999). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WI25 No PE/PPE Q11031 P9WI25 P9WI25 MMAR_4240 Mb1396c NC_000962.3 Mycobrowser_v4 CDS 1533948 1534610 . - 0 Rv1362c Rv1362c Function unknown Possible membrane protein Rv1362c, (MTCY02B10.26c), len: 220 aa. Possible membrane protein, similar to Mycobacterium tuberculosis hypothetical proteins e.g. Rv1362c|MTCY02B10.27c (25.9% identity in 216 aa overlap), Rv0177, Rv1973, Rv1972, etc. P9WM01 No cell wall and cell processes P9WM01 GO:0016021 P9WM01 MMAR_3995 MSMEG_4770 Mb1397c NC_000962.3 Mycobrowser_v4 CDS 1534607 1535392 . - 0 Rv1363c Rv1363c Function unknown Possible membrane protein Rv1363c, (MTCY02B10.27c), len: 261 aa. Possible membrane protein, similar to Mycobacterium tuberculosis hypothetical proteins Rv1362c|MTCY02B10.26c (25.9% identity in 216 aa overlap); Rv1972|MTV051.10 and Rv0177 etc. P9WLZ9 No cell wall and cell processes P9WLZ9 GO:0016021 P9WLZ9 MMAR_3994 MSMEG_4771 Mb1398c NC_000962.3 Mycobrowser_v4 CDS 1535683 1537644 . - 0 Rv1364c Rv1364c May be involved in regulating SIGF|Rv3286c Possible sigma factor regulatory protein Rv1364c, (MTCY02B10.28c), len: 653 aa. Possible sigma factor regulatory protein, some similarity to RSBU_BACSU|P40399 sigma factor sibg regulation protein from Bacillus subtilis (335 aa), FASTA scores: opt: 224, E(): 2e-07, (25.8% identity in 244 aa overlap). Also known as mursiF. P9WLZ7 No information pathways 3K3C,3K3D,3KE6,3KX0 Q11034 P9WLZ7 GO:0004721,GO:0006355,GO:0007165,GO:0000155,GO:0000160,GO:0005524 P9WLZ7 MMAR_3991 Mb1399c NC_000962.3 Mycobrowser_v4 CDS 1537783 1538169 . - 0 Rv1365c rsfA Regulates negatively Rv3287c|RSBW|USFX. Regulated by redox potential. Anti-anti-sigma factor RsfA (anti-sigma factor antagonist) (regulator of sigma F A) Rv1365c, (MTCY02B10.29c), len: 128 aa. RsfA, anti-anti-sigma factor (see citation below), similar to other Mycobacterium tuberculosis proteins e.g. Rv2638|MTCY441.08 (148 aa), FASTA scores: E(): 0, (53.6% identity in 125 aa overlap); Rv1904, Rv3687c. Weak similarity to putative anti-anti-sigma factors e.g. AF134889|AF134889_1 Streptomyces coelicolor (113 aa), FASTA scores: opt: 137, E(): 0.004, (26.0% identity in 100 aa overlap). P9WGE3 No information pathways Q11035 P9WGE3 GO:0006355,GO:0045152 P9WGE3 Mb1400c NC_000962.3 Mycobrowser_v4 CDS 1538390 1539211 . + 0 Rv1366 Rv1366 Unknown Hypothetical protein Rv1366, (MTCY02B10.30), len: 273 aa. Hypothetical unknown protein. P9WLZ5 No conserved hypotheticals P64833 P9WLZ5 GO:0015969 P9WLZ5 Mb1401 NC_000962.3 Mycobrowser_v4 CDS 1539180 1539440 . + 0 Rv1366A Rv1366A Unknown Conserved protein Rv1366A, len: 86 aa. Conserved protein. V5QQR7 No conserved hypotheticals V5QQR7 NC_000962.3 Mycobrowser_v4 CDS 2009172 2009990 . + 0 Rv1775 Rv1775 Function unknown Conserved hypothetical protein Rv1775, (MTCY25C11.02), unknown, len: 272 aa. Conserved hypothetical protein, similar to O28806|AF1466 conserved hypothetical protein from Archaeoglobus fulgidus (255 aa), FASTA scores: opt: 364, E(): 1e-17, (29.2% identity in 267 aa overlap). O33178 No conserved hypotheticals O33178 O33178 MSMEG_2484 Mb1804 NC_000962.3 Mycobrowser_v4 ncRNA 1547129 1547268 . + 0 MTB000076 MTS1082 Unknown Putative small regulatory RNA MTS1082, putative small regulatory RNA (See Arnvig et al., 2011), ends not mapped, ~150 bp band detected by Northern blot. No stable RNAs NC_000962.3 Mycobrowser_v4 CDS 1547072 1547530 . - 0 Rv1374c Rv1374c Unknown Hypothetical protein Rv1374c, (MTCY02B12.08c), len: 152 aa. Hypothetical unknown protein. P71802 No conserved hypotheticals P71802 P71802 Mb1409c NC_000962.3 Mycobrowser_v4 CDS 1549148 1550641 . + 0 Rv1376 Rv1376 Function unknown Conserved hypothetical protein Rv1376, (MTCY02B12.10), len: 497 aa. Conserved hypothetical protein, some similarity to hypothetical proteins from several organisms e.g. Q52872|U39409 Rhizobium leguminosarum (247 aa), FASTA scores: E(): 2.1e-12, (34.7% identity in 219 aa overlap). P71804 No conserved hypotheticals P71804 P71804 P71804 ML1353,ML1353c MMAR_2493 Mb1411 NC_000962.3 Mycobrowser_v4 CDS 1542929 1543255 . - 0 Rv1370c Rv1370c Possibly required for the transposition of the insertion element IS6110. Putative transposase for insertion sequence element IS6110 (fragment) Rv1370c, (MTCY02B12.04c), len: 108 aa. Putative transposase for IS6110 (fragment), identical to many other Mycobacterium tuberculosis IS6110 transposase subunits e.g. Q50686|YIA4_MYCTU Insertion element IS6110 hypothetical 12.0 kDa protein (108 aa), fasta scores: E(): 1.4e-43, (100.00% identity in 108 aa overlap). The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv1368c and Rv1369c, the sequence UUUUAAAG (directly upstream of Rv1369c) maybe responsible for such a frameshifting event (see McAdam et al., 1990). P9WKH5 No insertion seqs and phages P0C5G9 P9WKH5 GO:0004803,GO:0006313,GO:0003677 P9WKH5 NC_000962.3 Mycobrowser_v4 CDS 1543359 1544828 . + 0 Rv1371 Rv1371 Unknown Probable conserved membrane protein Rv1371, (MTCY02B12.05), len: 489 aa. Probable membrane protein. Weak similarity to delta 5 fatty acid desaturases e.g. AB022097|AB022097_1 Dictyostelium discoideum (467 aa), FASTA score: opt: 173, E(): 0.00052, (22.4% identity in 438 aa overlap); and Homo sapiens. P71799 No cell wall and cell processes P71799 P71799 GO:0006629,GO:0055114,GO:0016491,GO:0020037 MMAR_2189 Mb1404,Mb1405 NC_000962.3 Mycobrowser_v4 CDS 1544825 1546006 . + 0 Rv1372 Rv1372 Function unknown Conserved hypothetical protein Rv1372, (MTCY02B12.06), len: 393 aa. Conserved hypothetical protein, similar to several chalcone synthases e.g. CHS2_GERHY|P48391 chalcone synthase 2 from gerbra hybrid (402 aa), FASTA scores: opt: 511, E(): 7e-26, (28.4% identity in 380 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical chalcone synthases, Rv1665, Rv1660. P9WPF1 No conserved hypotheticals 1TED,1TEE Q7D8I1 P9WPF1 GO:0009058,GO:0008415 P9WPF1 MMAR_2190 Mb1406 NC_000962.3 Mycobrowser_v4 CDS 1546012 1546992 . + 0 Rv1373 Rv1373 Involved in sulfation: activity towards typical ceramide glycolipids and trehalose glycolipids. Glycolipid sulfotransferase Rv1373, (MTCY02B12.07), len: 326 aa. Glycolipid sulfotransferase (see citation below); slight similarity to sulfotransferases e.g. SUOE_CAVPO|P49887 estrogen sulfotransferase from Cavia porcellus (Guinea pig) (296 aa), FASTA scores, opt: 165, E():0.00054, (24.5% identity in 294 aa overlap). P9WGB9 No intermediary metabolism and respiration P71801 P9WGB9 GO:0008146 2.8.2.- P9WGB9 MMAR_2192 Mb1407,Mb1408 NC_000962.3 Mycobrowser_v4 CDS 1695281 1695946 . - 0 Rv1505c Rv1505c Function unknown Conserved hypothetical protein Rv1505c, (MTCY277.27c), len: 221 aa. Conserved hypothetical protein, some similarity to hypothetical proteins and glycosylases e.g. P71063|O08181 hypothetical 22.5 kDa protein YVFD from Bacillus subtilis (216 aa), FASTA scores: E(): 2.4e-08, (25.5% identity in 196 aa overlap). P71784 No conserved hypotheticals P71784 GO:0016740 P71784 MMAR_2321 Mb1543c NC_000962.3 Mycobrowser_v4 CDS 2367359 2367655 . + 0 Rv2107 PE22 Function unknown PE family protein PE22 Rv2107, (MTCY261.03), len: 98 aa. PE22, Member of mycobacterial PE family (see citation below). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). L7N6B5 No PE/PPE Q7D7I5 L7N6B5 L7N6B5 Mb2131 NC_000962.3 Mycobrowser_v4 CDS 1552654 1553235 . + 0 Rv1379 pyrR Binds to the conserved sequence in the PYR operon mRNA and disrupts the antiterminator, permitting terminator hairpin formation and promoting transcription termination Probable pyrimidine operon regulatory protein PyrR Rv1379, (MTCY02B12.13), len: 193 aa. Probable pyrR, pyrimidine operon regulatory protein, similar to PYRR_BACCL|P41007 pyrimidine operon regulatory protein from Bacillus caldolyticus (179 aa), FASTA scores: opt: 544, E(): 1.1e-30, (54.2% identity in 179 aa overlap). P9WHK3 No regulatory proteins 1W30 P65941 P9WHK3 GO:0045449,GO:0006350,GO:0004845,GO:0009116 2.4.2.9 P9WHK3 ML0531 MMAR_2194 MSMEG_3042 Mb1414 NC_000962.3 Mycobrowser_v4 CDS 1554188 1555480 . + 0 Rv1381 pyrC Involved in pyrimidine biosynthesis (third step) [catalytic activity: (S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate] Probable dihydroorotase PyrC (DHOase) Rv1381, (MTCY02B12.15), len: 430 aa. Probable pyrC, dihydroorotase, similar to many e.g. PYRC_BACCL|P46538 (40.5% identity in 395 aa overlap). Contains PS00483 Dihydroorotase signature 2. Belongs to the DHOase family. subfamily 2. P9WHL3 No intermediary metabolism and respiration P71809 P9WHL3 GO:0046872,GO:0006221,GO:0004151 3.5.2.3 P9WHL3 ML0533 MMAR_2196 MSMEG_3044 Mb1416 NC_000962.3 Mycobrowser_v4 CDS 1555477 1555974 . + 0 Rv1382 Rv1382 Unknown Probable export or membrane protein Rv1382, (MTCY02B12.16), len: 165 aa. Possible exported or membrane protein, hydrophobic domain at N-terminus. Predicted to be an outer membrane protein (See Song et al., 2008). P71810 No cell wall and cell processes P71810 ML0534 MMAR_2197 MSMEG_3045 Mb1417 NC_000962.3 Mycobrowser_v4 CDS 1744426 1744836 . - 0 Rv1542c glbN Oxygen transport Hemoglobin GlbN Rv1542c, (MTCY48.23), len: 136 aa. glbN, hemoglobin. Belongs to the protozoan/cyanobacterial globin family. Similar to myoglobins e.g. GLB_PARCA|P15160 myoglobin (hemoglobin) paramecium (116 aa), FASTA scores, opt: 284, E(): 2.1e -13, (35.7% identity in 115 aa overlap). Similar to Mycobacterium tuberculosis hypothetical globin, Rv2470. P9WN25 No intermediary metabolism and respiration 1IDR,1RTE,1S56,1S61,2GKM,2GKN,2GL3,2GLN P0A592 P9WN25 GO:0019825,GO:0015671,GO:0005344,GO:0020037 P9WN25 ML1203,ML1203c MMAR_2365 MSMEG_5765 Mb1569c NC_000962.3 Mycobrowser_v4 CDS 2217659 2218036 . + 0 Rv1974 Rv1974 Unknown Probable conserved membrane protein Rv1974, (MTV051.12), len: 125 aa. Probable conserved membrane protein, weakly similar to other Mycobacterium tuberculosis proteins e.g. Rv1271c|Z77137|MTCY50.11 (113 aa), FASTA scores: opt: 98, E(): 1.4, (24.5% identity in 110 aa overlap); Rv1804c; Rv1690. Has possible signal peptide or transmembrane stretch from aa 12-30. Predicted to be an outer membrane protein (See Song et al., 2008). O53975 No cell wall and cell processes O53975 O53975 MSMEG_0354 NC_000962.3 Mycobrowser_v4 CDS 1560445 1561269 . + 0 Rv1385 pyrF Involved in the biosynthesis of pyrimidines [catalytic activity: orotidine 5'-phosphate = UMP + CO(2)] Probable orotidine 5'-phosphate decarboxylase PyrF (OMP decarboxylase) (ompdecase) Rv1385, (MTCY21B4.02), len: 274 aa. Probable pyrF, orotidine 5'-phosphate decarboxylase, identical to DCOP_MYCBO|P42610 Mycobacterium bovis (274 aa). Contains PS00156 Orotidine 5'-phosphate decarboxylase active site. Belongs to the OMP decarboxylase family. P9WIU3 No intermediary metabolism and respiration P0A5M6 P9WIU3 GO:0004590,GO:0006221,GO:0006207 4.1.1.23 P9WIU3 ML0537 MMAR_2200 MSMEG_3048 Mb1420 NC_000962.3 Mycobrowser_v4 CDS 1561464 1561772 . + 0 Rv1386 PE15 Function unknown PE family protein PE15 Rv1386, (MTCY21B4.03), len: 102 aa. PE15, Member of Mycobacterium tuberculosis PE family (see Brennan & Delogu 2002), similar to many e.g. G913039 ORF 3' of PGRS tandem repeat (polymorphic GC-rich sequence) (100 aa), FASTA scores: opt: 149, E(): 0.0013, (31.5% identity in 92 aa overlap); also similar to Q49943|U1756A (99 aa) (34.7% identity in 95 aa overlap) and G466937|U1620K (100 aa) (36.2% identity in 69 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). P9WIH1 No PE/PPE P0A682 P9WIH1 P9WIH1 ML0538 MMAR_5258 MSMEG_0618 Mb1421 NC_000962.3 Mycobrowser_v4 CDS 1563694 1564266 . + 0 Rv1388 mihF Unknown Putative integration host factor MihF Rv1388, (MTCY21B4.05), len: 190 aa. Putative mihF, integration host factor. Almost identical to, but longer than, P96802|U75344 Mycobacterium smegmatis integration host factor (mIHF) for mycobacteriophage L5 (105 aa), FASTA scores: E(): 0, (96.1% identity in 102 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P71658 No information pathways P71658 P71658 ML0540 Mb1423 NC_000962.3 Mycobrowser_v4 CDS 1564401 1565027 . + 0 Rv1389 gmk Essential for recycling GMP and indirectly, CGMP [catalytic activity: ATP + GMP = ADP + GDP] Probable guanylate kinase Gmk Rv1389, (MTCY21B4.06), len: 208 aa. Probable gmk, guanylate kinase, similar to e.g. KGUA_ECOLI|P24234 guanylate kinase from Escherichia coli (207 aa), FASTA scores: opt: 424, E(): 6.6e-20, (35.9% identity in 184 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00856 Guanylate kinase signature. Belongs to the guanylate kinase family. P9WKE9 No intermediary metabolism and respiration 1S4Q,1Z8F,1ZNW,1ZNX,1ZNY,1ZNZ P0A5I4 P9WKE9 GO:0005737,GO:0004385,GO:0006163,GO:0005524 2.7.4.8 P9WKE9 ML0541 MMAR_2202 MSMEG_3051 Mb1424 NC_000962.3 Mycobrowser_v4 CDS 1565093 1565425 . + 0 Rv1390 rpoZ Promotes RNA polymerase assembly. Latches the N-and C-terminal regions of the beta' subunit thereby faciltating its interaction with the beta and alpha subunits [catalytic activity: N nucleoside triphosphate = N diphosphate + {RNA}N]. Probable DNA-directed RNA polymerase (omega chain) RpoZ (transcriptase omega chain) (RNA polymerase omega subunit) Rv1390, (MTCY21B4.07), len: 110 aa. Probable rpoZ, DNA-directed RNA polymerase omega chain. Belongs to the RNA polymerase omega chain family. P9WGY5 No information pathways P66721 P9WGY5 GO:0003899,GO:0006351,GO:0003677 2.7.7.6 P9WGY5 ML0542 MMAR_2203 MSMEG_3053 Mb1425 NC_000962.3 Mycobrowser_v4 CDS 1583101 1584474 . + 0 Rv1407 fmu Unknown Probable Fmu protein (sun protein) Rv1407, (MTCY21B4.24), len: 457 aa. Probable fmu protein, similar to SUN_ECOLI|P36929 sun protein (fmu protein) from Escherichia coli (429 aa), FASTA scores: E(): 2.5e-20, (30.6% identity in 451 aa overlap). P9WGX3 No information pathways P71675 P9WGX3 GO:0008168,GO:0006355,GO:0003723 2.1.1.- P9WGX3 ML0553 MMAR_2216 MSMEG_3065 Mb1442 NC_000962.3 Mycobrowser_v4 CDS 1764979 1765383 . + 0 Rv1561 vapC11 Unknown Possible toxin VapC11 Rv1561, (MTCY48.04c), len: 134 aa. Possible vapC11, toxin, part of toxin-antitoxin (TA) operon with Rv1560, contains PIN domain, (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others from Mycobacterium tuberculosis e.g. Q10847|Rv2010|MTCY39.07c (132 aa), FASTA scores: (37.0% identity in 127 aa overlap); and O06566|Rv1114|MTCY22G8.03 (124 aa). P9WFA5 No virulence, detoxification, adaptation P64879 P9WFA5 P9WFA5 Mb1587 NC_000962.3 Mycobrowser_v4 CDS 1568109 1569587 . - 0 Rv1393c Rv1393c Function unknown; probably involved in cellular metabolism Probable monoxygenase Rv1393c, (MTCY21B4.10c), len: 492 aa. Probable monooxygenase, similar to others e.g. CYMO_ACISP|P12015 cyclohexanone monooxygenase from Acinetobacter sp. (542 aa), FASTA scores: E(): 0, (33.0% identity in 473 aa overlap); also to Rv3083|MTCY31.20|E241788 hypothetical 55.0 kDa protein from Mycobacterium tuberculosis (495 aa) (36.3% identity in 490 aa overlap); and Rv0565c, Rv3854c, Rv3049c, Rv0892. P71662 No intermediary metabolism and respiration P71662 P71662 GO:0055114,GO:0004497 1.14.13.- P71662 MMAR_2207 Mb1428c NC_000962.3 Mycobrowser_v4 CDS 1569584 1570969 . - 0 Rv1394c cyp132 Cytochromes P450 are a group of heme-thiolate monooxygenases. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Probable cytochrome P450 132 Cyp132 Rv1394c, (MT1439, MTCY21B4.11c), len: 461 aa. Probable cyp132, cytochrome P450 132. Some similarity to others e.g. CP4B_HUMAN|P13584 human cytochrome p450 (511 aa), FASTA scores: opt: 486, E(): 7.4e-21, (28.6% identity in 423 aa overlap); etc. Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. May belong to the cytochrome P450 family. Experimentally shown that the expression of cyp132 is induced by the transcriptional regulatory protein Rv1395 (Recchi et al., 2003). P9WPN3 No intermediary metabolism and respiration P77900 P9WPN3 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPN3 Mb1429c NC_000962.3 Mycobrowser_v4 CDS 1574850 1575809 . - 0 Rv1399c nlhH Function unknown, but supposedly involved in lipid metabolism Probable non lipolytic carboxylesterase NlhH Rv1399c, (MTCY21B4.16c), len: 319 aa. Possible nlhH, non lipolytic carboxylesterase, most similar to G695278 lipase like enzyme from Ralstonia eutropha (364 aa), FASTA scores: opt: 648, E(): 4.4e-34, (37.3% identity in 327 aa ov erlap), similar to Mycobacterium tuberculosis hypothetical lipases e.g. Rv2284, Rv2485c, Rv1426c, etc. Previously known as lipH. P9WK87 No intermediary metabolism and respiration P71667 P9WK87 GO:0016787 3.1.-.- P9WK87 ML0545,ML0545c MMAR_2208 Mb1434c NC_000962.3 Mycobrowser_v4 CDS 1589891 1590292 . + 0 Rv1414 Rv1414 Function unknown Conserved hypothetical protein Rv1414, (MTCY21B4.31), len: 133 aa. Conserved hypothetical protein, similar to C-terminal part of AB010956|AB010956_1 novel metal-activated pyridoxal enzyme from Arthrobacter sp. (379 aa), FASTA scores: opt: 163, E(): 0.00063, (32.1% identity in 112 aa overlap). Rv1413 is similar to N-terminal part of same enzyme suggesting possible frameshift. Sequence has been checked and no errors found, it is identical in Mycobacterium bovis strain AF2122/97 and in Mycobacterium tuberculosis CDC1551. P9WLY3 No conserved hypotheticals P9WLY3 P9WLY3 Mb1449 NC_000962.3 Mycobrowser_v4 CDS 1599658 1601037 . + 0 Rv1425 Rv1425 May be involved in synthesis of triacylglycerol Possible triacylglycerol synthase (diacylglycerol acyltransferase) Rv1425, (MTCY21B4.43,MTCY493.29c), len: 459 aa. Possible triacylglycerol synthase (See Daniel et al., 2004), similar to many M. tuberculosis proteins e.g. Rv3740c, Rv3734c, Rv1760, etc. P9WKC1 No lipid metabolism P71694 P9WKC1 GO:0006071,GO:0008610,GO:0004144 2.3.1.20 P9WKC1 ML0566 MMAR_2231 Mb1460 NC_000962.3 Mycobrowser_v4 CDS 1572127 1573857 . - 0 Rv1396c PE_PGRS25 Function unknown PE-PGRS family protein PE_PGRS25 Rv1396c, (MTCY21B4.13c), len: 576 aa. PE_PGRS25, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002), strong similarity to many e.g. glycine rich protein MTCY130.10C|E245019 (603 aa), FASTA scores: opt: 1945, E(): 0, (57.5% identity in 619 aa overlap). Contains PS00017 ATP/GTP-binding site motif A, similar to other PGRS-type sequences. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P71664 No PE/PPE P71664 P71664 P71664 MMAR_0572,MMAR_2053,MMAR_2248,MMAR_3199,MMAR_4127,MMAR_4223,MMAR_4224,MMAR_4548 Mb1431c NC_000962.3 Mycobrowser_v4 CDS 1574112 1574513 . - 0 Rv1397c vapC10 Unknown Possible toxin VapC10 Rv1397c, (MTCY21B4.14c), len: 133 aa. Possible vapC10, toxin, part of toxin-antitoxin (TA) operon with Rv1398c, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Conserved hypothetical protein, similar to Mycobacterium tuberculosis protein MTCY159.08C|Rv2548 (125 aa), FASTA scores: E(): 2.3e-14, (42.4% identity in 125 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WFA7 No virulence, detoxification, adaptation P71665 P9WFA7 P9WFA7 Mb1432c NC_000962.3 Mycobrowser_v4 CDS 1574510 1574767 . - 0 Rv1398c vapB10 Unknown Possible antitoxin VapB10 Rv1398c, (MTCY21B4.15c), len: 85 aa. Possible vapB10, antitoxin, part of toxin-antitoxin (TA) operon with Rv1397c (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. Rv2547|MTCY159.09C (85 aa), FASTA scores: E(): 0.0035, (37.1% identity in 62 aa overlap); Rv0581, Rv2871, Rv1241, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLZ1 No virulence, detoxification, adaptation P64835 P9WLZ1 GO:0006355,GO:0003677 P9WLZ1 Mb1433c NC_000962.3 Mycobrowser_v4 CDS 1575834 1576796 . - 0 Rv1400c lipI Function unknown, but possibly involved in lipid metabolism Probable lipase LipH Rv1400c, (MTCY21B4.17c), len: 320 aa. Possible lipI, lipase, most similar to G695278 lipase like enzyme (364 aa), FASTA sscores: opt: 611, E(): 3.5e-30, (36.6% identity in 352 aa overlap); similar to M. tuberculosis hypothetical lipases e.g. Rv1399c|MTCY21B4.16c (58.1% identical in 315 aa overlap); Rv1426c, Rv2284, etc. P71668 No intermediary metabolism and respiration P71668 P71668 GO:0008152,GO:0016787 3.1.-.- P71668 ML0546,ML0546c MMAR_2209 MSMEG_3059 Mb1435c NC_000962.3 Mycobrowser_v4 CDS 1576930 1577532 . + 0 Rv1401 Rv1401 Unknown Possible membrane protein Rv1401, (MTCY21B4.18), len: 200 aa. Possible membrane protein. P9WG51 No cell wall and cell processes P64837 P9WG51 GO:0005886,GO:0016021 ML0547 MMAR_2210 MSMEG_3060 Mb1436 NC_000962.3 Mycobrowser_v4 CDS 1577613 1579580 . + 0 Rv1402 priA Recognizes a specific hairpin sequence on PHIX SSDNA; this structure is then recognized and bound by proteins PRIB and PRIC. Formation of the primosome proceeds with the subsequent actions of DNAB, DNAC, DNAT and primase. PRIA then functions as a helicase within the primosome Putative primosomal protein N' PriA (replication factor Y) Rv1402, (MTCY21B4.19), len: 655 aa. Putative priA, primosomal protein N'. Similar to e.g. PRIA_ECOLI|P17888 primosomal protein N' (replication factor Y) (732 aa), FASTA scores, opt: 386, E(): 1.3e-16, (27.6% identity in 711 aa overlap). Compared to other bacterial priA, it has a very divergent helicase domain. Belongs to the helicase family. PRIA subfamily. P9WMQ9 No information pathways P9WMQ9 GO:0003677,GO:0006269,GO:0005658,GO:0004386,GO:0046872,GO:0005524 3.6.4.- P9WMQ9 ML0548 MMAR_2211 MSMEG_3061 Mb1437 NC_000962.3 Mycobrowser_v4 CDS 1695943 1696443 . - 0 Rv1506c Rv1506c Unknown Hypothetical protein Rv1506c, (MTCY277.28c), len: 166 aa. Hypothetical unknown protein. P71785 No unknown P71785 P71785 P71785 MMAR_2322 NC_000962.3 Mycobrowser_v4 CDS 1580591 1581073 . + 0 Rv1404 Rv1404 Involved in transcriptional mechanism Probable transcriptional regulatory protein Rv1404, (MTCY21B4.21), len: 160 aa. Probable transcriptional regulatory protein, some similarity to MARR_ECOLI|P27245 multiple antibiotic resistance protein from Escherichia coli (125 aa), FASTA scores: opt: 136, E(): 0.004, (35.1% identity in 74 aa overlap). P71672 No regulatory proteins 2NYX P71672 P71672 GO:0006350,GO:0006355,GO:0003700,GO:0005622 P71672 ML0550 MSMEG_5566 Mb1439 NC_000962.3 Mycobrowser_v4 CDS 1581145 1581969 . - 0 Rv1405c Rv1405c Causes methylation Putative methyltransferase Rv1405c, (MTCY21B4.22c), len: 274 aa. Putative methyltransferase, most similar to PMTA_RHOSH|Q05197 phosphatidylethanolamine m-methyltransferase (203 aa), FASTA scores: opt: 219, E(): 2.6e-07, (29.9% identity in 144 aa overlap); similar to Rv1403c|MTCY21B4.20c (59.3% identity in 273 aa overlap), Rv1523, Rv2952, etc. P9WLY7 No intermediary metabolism and respiration P64841 P9WLY7 GO:0008168,GO:0008152 P9WLY7 ML0551,ML0551c MMAR_2214 Mb1440c NC_000962.3 Mycobrowser_v4 CDS 1584499 1585197 . + 0 Rv1408 rpe Involved in the calvin cycle [catalytic activity: D-ribulose 5-phosphate = D-xylulose 5- phosphate] Probable ribulose-phosphate 3-epimerase Rpe (PPE) (R5P3E) (pentose-5-phosphate 3-epimerase) Rv1408, (MTCY21B4.25), len: 232 aa. Probable rpe, ribulose-phosphate 3-epimerase, similar to many e.g. CXEC_ALCEU|P40117 (241 aa), FASTA scores: opt: 638, E(): 1.5e-34, (48.3% identity in 234 aa overlap); and RPE_ECOLI|P32661 ribulose-phosphate 3-epimerase (225 aa), FASTA scores: E(): 0, (46.2% identity in 221 aa overlap). Contains PS01085 Ribulose-phosphate 3-epimerase family signature 1. Belongs to the ribulose-phosphate 3-epimerase family. P9WI51 No intermediary metabolism and respiration P65760 P9WI51 GO:0004750,GO:0005975 5.1.3.1 P9WI51 ML0554 MMAR_2217 MSMEG_3066 Mb1443 NC_000962.3 Mycobrowser_v4 CDS 1601059 1602321 . - 0 Rv1426c lipO Function unknown, but supposedly involved in lipid metabolism Probable esterase LipO Rv1426c, (MTCY493.28), len: 420 aa. Possible lipO, esterase, similar to several Mycobacterium tuberculosis hypothetical lipases and esterases e.g. Rv1399c, Rv2284, etc. Also similar in central region to AAAD_HUMAN|P22760 human arylacetamide deacetylase (398 aa), FASTA scores: opt:210, E(): 7.6e-07, (29.3% identity in 191 aa overlap). O06832 No intermediary metabolism and respiration O06832 O06832 GO:0016787 3.1.-.- O06832 MMAR_2232 MSMEG_0302 Mb1461c NC_000962.3 Mycobrowser_v4 CDS 1608083 1609852 . + 0 Rv1431 Rv1431 Unknown Conserved membrane protein Rv1431, (MTCY493.23c), len: 589 aa. Conserved membrane protein, shows strong similarity to another M. tuberculosis hypothetical protein Rv1132|MTCY22G8.21 (48.2% identity in 585 aa overlap). O06827 No cell wall and cell processes O06827 O06827 ML0567 MMAR_2236 Mb1466 NC_000962.3 Mycobrowser_v4 CDS 1586210 1587766 . - 0 Rv1410c Rv1410c Involved in transport of aminoglycosides and tetracycline across the membrane (export): drug resistance by an export mechanism (conferes resistance to toxic compounds by removing them for the cells). Responsible for the translocation of the substrate across the membrane. Aminoglycosides/tetracycline-transport integral membrane protein Rv1410c, (MTCY21B4.27c), len: 518 aa. Aminoglycoside/tetracycline-transport integral membrane protein (see citation below), member of major facilitator superfamily (MFS), similar to others e.g. AC22_STRCO|P46105 probable actinorhodin transporter from Streptomyces coelicolor (578 aa), FASTA scores: opt: 442, E(): 4.9e-21, (28.5% identity in 466 aa overlap); etc. Contains PS00216 Sugar transport proteins signature 1. Could be termed P55. Note that the Rv1410c-Rv1411c operon seems transcribed from two promoters in Mycobacterium bovis BCG (see Bigi et al., 2000). P9WJY3 No cell wall and cell processes P71678 P9WJY3 GO:0005886,GO:0055085,GO:0005215,GO:0016021 ML0556,ML0556c MMAR_2219 MSMEG_3069 Mb1445c NC_000962.3 Mycobrowser_v4 CDS 1588567 1589172 . + 0 Rv1412 ribC Involved in riboflavin synthesis. Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyzes the dismutation of 6,7-dimethyl-8-lumazine to riboflavin and 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)- pyrimidinedione. Probable riboflavin synthase alpha chain RibC (RibE) Rv1412, (MTCY21B4.29), len: 201 aa. Probable ribC (ribE), Riboflavin synthase alpha chain, strong similarity to e.g. RISA_ACTPL|P50854 (215 aa), FASTA scores: opt: 586, E(): 1.8e-33, (50.8% identity in 197 aa overlap). Contains 2 x PS00693 Riboflavin synthase alpha chain family signature. P9WK35 No intermediary metabolism and respiration P65327 P9WK35 GO:0004746,GO:0009231 2.5.1.9 P9WK35 ML0558 MMAR_2221 MSMEG_3071 Mb1447 NC_000962.3 Mycobrowser_v4 CDS 1590397 1591674 . + 0 Rv1415 ribA2 Involved in riboflavin biosynthesis [catalytic activity: GTP + 3 H(2)O = formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate]. Probable riboflavin biosynthesis protein RibA2 : GTP cyclohydrolase II + 3,4-dihydroxy-2-butanone 4-phosphate synthase (DHBP synthase) Rv1415, (MTCY21B4.33), len: 425 aa. Probable ribA2, Riboflavin biosynthesis protein, similar to many e.g. GCH2_BACSU|P17620 Bacillus subtilis (398 aa), FASTA scores: opt: 1388, E(): 0, (55.4% identity in 399 aa overlap). Also similar to second Mycobacterium tuberculosis gtp cyclohydrolase Rv1940|ribA1 (353 aa). In the N-terminal section; belongs to the DHBP synthase family. In the C-terminal section; belongs to the GTP cyclohydrolase II family. P9WHF1 No intermediary metabolism and respiration P0A5V0 P9WHF1 GO:0005525,GO:0003935,GO:0000287,GO:0030145,GO:0009231,GO:0008270,GO:0008686 3.5.4.25,4.1.99.12 P9WHF1 ML0559 MMAR_2222 MSMEG_3072 Mb1450 NC_000962.3 Mycobrowser_v4 CDS 1623287 1623697 . - 0 Rv1444c Rv1444c Unknown Unknown protein Rv1444c, (MTCY493.10), len: 136 aa. Unknown protein. O06815 No conserved hypotheticals O06815 MMAR_2249 MSMEG_3098 Mb1479c NC_000962.3 Mycobrowser_v4 CDS 1596881 1597909 . + 0 Rv1422 Rv1422 Function unknown Conserved hypothetical protein Rv1422, (MTCY21B4.40), len: 342 aa. Conserved hypothetical protein, similar to many hypothetical proteins e.g. YAMB_THETU|P38541 Thermoanaerobacterium thermosulfurigenes (323 aa), FASTA scores: opt: 519, E(): 1.6e-25, (33.1% identity in 320 aa overlap); and AF106003|AF106003_3 Streptomyces coelicolor (363 aa), FASTA scores: opt: 1047, E(): 0, (54.5% identity in 308 aa overlap). P9WMU5 No conserved hypotheticals P71691 P9WMU5 P9WMU5 ML0564 MMAR_2229 MSMEG_3080 Mb1457 NC_000962.3 Mycobrowser_v4 CDS 1598893 1599654 . - 0 Rv1424c Rv1424c Unknown Possible membrane protein Rv1424c, (MTCY21B4.42c,MTCY493.30), len: 253 aa. Possible membrane protein, contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLX7 No cell wall and cell processes P9WLX7 Mb1459c NC_000962.3 Mycobrowser_v4 CDS 1602321 1603928 . - 0 Rv1427c fadD12 Function unknown, but supposed involvement in lipid degradation. Possible long-chain-fatty-acid--CoA ligase FadD12 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv1427c, (MTCY493.27), len: 535 aa. Possible fadD12, long-chain-fatty-acid-CoA synthetase, similar to many e.g. NP_302632.1|NC_002677 acyl-CoA synthase from Mycobacterium leprae (548 aa); AAD01929.2|AF031419 putative long-chain-fatty-acid--CoA ligase from Pseudomonas putida (565 aa); NP_419782.1|NC_002696 putative long-chain-fatty-acid--CoA ligase from Caulobacter crescentus (530 aa); PC60_YEAST|P38137 yeast peroxisomal-coenzyme A synthetase (543 aa), FASTA scores: opt: 507, E(): 2.9e-25, (30.4% identity in 365 aa overlap). Also similar to many M. tuberculosis proteins e.g. MTCY06A4.14 (44.8% identity in 525 aa overlap). Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family. O06831 No lipid metabolism O06831 O06831 GO:0008152,GO:0016874 6.2.1.- O06831 MMAR_2233 MSMEG_0304 Mb1462c NC_000962.3 Mycobrowser_v4 CDS 1603932 1604759 . - 0 Rv1428c Rv1428c Function unknown Conserved hypothetical protein Rv1428c, (MTCY493.26), len: 275 aa. Conserved hypothetical protein, some similarity to hypothetical proteins from Mycobacterium tuberculosis e.g. Rv0502|YV29_MYCTU|Q11167 (358 aa), FASTA scores: opt: 355, E(): 5e-16, (32.6% identity in 273 aa overlap); and Rv1920. O06830 No conserved hypotheticals O06830 O06830 GO:0008152,GO:0008415 O06830 MMAR_2234 MSMEG_0305 Mb1463c NC_000962.3 Mycobrowser_v4 CDS 2387202 2387972 . - 0 Rv2126c PE_PGRS37 Function unknown PE-PGRS family protein PE_PGRS37 Rv2126c, (MTCY261.22c), len: 256 aa. PE_PGRS37, Possible PE_PGRS pseudogene fragment, similar to the Gly-rich C-terminus of many members of the Mycobacterium tuberculosis PGRS family. L0TBL4 No PE/PPE L0TBL4 Mb2150c NC_000962.3 Mycobrowser_v4 CDS 3177822 3178085 . + 0 Rv2866 relG Function unknown Toxin RelG Rv2866, (MTV003.12), len: 87 aa. RelG, toxin, part of toxin-antitoxin (TA) operon with Rv2865 (See Pandey and Gerdes, 2005), similar to O50461|Rv1246c|MTV006.18c conserved hypothetical protein from Mycobacterium tuberculosis (97 aa), FASTA scores: opt: 290, E(): 3.6e-16, (54.1% identity in 85 aa overlap). O33348 No virulence, detoxification, adaptation 3G5O O33348 O33348 O33348 ML1579 Mb2891 NC_000962.3 Mycobrowser_v4 CDS 1604878 1606146 . + 0 Rv1429 Rv1429 Function unknown Conserved protein Rv1429, (MTCY493.25c), len: 422 aa. Conserved protein, some similarity to transcriptional regulator proteins e.g. CDAR_ECOLI|P37047 Carbohydrate diacid regulator from Escherichia coli (391 aa), FASTA scores: opt: 210, E(): 3e-06, (27.7% identity in 296 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins Rv2370c, Rv1194c, Rv1453, Rv2242, and Rv1186c. O06829 No conserved hypotheticals O06829 O06829 MMAR_1582,MMAR_1603 Mb1464 NC_000962.3 Mycobrowser_v4 CDS 1609849 1611270 . + 0 Rv1432 Rv1432 Function unknown; probably involved in cellular metabolism Probable dehydrogenase Rv1432, (MTCY493.22c), len: 473 aa. Probable dehydrogenase, shows strong similarity to P49_STRLI|P06108 p49 protein from Streptomyces lividans (469 aa), FASTA scores: opt: 1362, E(): 0, (44.9% identity in 474 aa overlap); and weak similarity to other dehydrogenases. O06826 No intermediary metabolism and respiration O06826 O06826 GO:0016491,GO:0055114 1.-.-.- O06826 MMAR_2237 Mb1467 NC_000962.3 Mycobrowser_v4 CDS 1611434 1612249 . + 0 Rv1433 Rv1433 Unknown Possible conserved exported protein Rv1433, (MTCY493.21c), len: 271 aa. Possible exported protein with N-terminal signal sequence, highly similar to Q49706 hypothetical protein from Mycobacterium leprae (271 aa), FASTA scores: opt: 1341, E(): 0, (68.3% identity in 271 aa overlap). Also shows similarity to M. tuberculosis lipoprotein Rv2518c|MTV009.03c lppS (408 aa) (40.0% identity in 230 aa overlap); and others e.g. Rv0116c, Rv0192, Rv2518c, Rv0483. Predicted to be an outer membrane protein (See Song et al., 2008). O06825 No cell wall and cell processes O06825 O06825 O06825 ML0569,ML0569c MMAR_3552 MSMEG_0674 Mb1468 NC_000962.3 Mycobrowser_v4 CDS 1623714 1624457 . - 0 Rv1445c devB Involved in pentose phosphate pathway. Hydrolysis of 6-phosphogluconolactone to 6- phosphogluconate. [catalytic activity: 6-phospho-D-glucono-1,5-lactone + H(2)O = 6- phospho-D-gluconate] Probable 6-phosphogluconolactonase DevB (6PGL) Rv1445c, (MTCY493.09), len: 247 aa. Possible devB (PGL), 6-phosphogluconolactonase, belongs to a different family to the upstream gene zwf2. Similar to e.g. DEVB_ANASP|P46016 putative glucose-6-phosphate 1-dehydrogenase (239 aa), FASTA scores: opt: 439, E(): 2.6e-20, (34.0% identity in 247 aa overlap). Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily. P9WQP5 No intermediary metabolism and respiration 3ICO P63338 P9WQP5 GO:0006098,GO:0017057 3.1.1.31 P9WQP5 ML0579,ML0579c MMAR_2250 MSMEG_3099 Mb1480c NC_000962.3 Mycobrowser_v4 CDS 1652768 1653256 . + 0 Rv1465 Rv1465 Function unknown; probably involved in cellular metabolism. Possible nitrogen fixation related protein Rv1465, (MTV007.12), len: 162 aa. Possible nitrogen fixation related protein. Equivalent to Z99125|MLCL536.24c nitrogen fixation protein NIFU from Mycobacterium leprae (165 aa), FASTA scores: opt: 870, E(): 0, (81.8% identity in 165 aa overlap). Also similar to O32163|Z99120|NIFU_BACSU NifU-like protein from Bacillus subtilis (147 aa), FASTA scores: opt: 354, E(): 4.1e-17, (38.3% identity in 141 aa overlap) and to AL096839|SCC22.02 hypothetical protein from Streptomyces coelicolor (156 aa), FASTA scores: opt: 569, E(): 1.2e-31, (56.3% identity in 158 aa overlap). O53156 No intermediary metabolism and respiration O53156 O53156 GO:0016226,GO:0051536,GO:0005515,GO:0005506 O53156 ML0597 MMAR_2270 MSMEG_3126 Mb1500 NC_000962.3 Mycobrowser_v4 CDS 1846716 1846973 . - 0 Rv1638A Rv1638A Function unknown Conserved hypothetical protein Rv1638A, len: 85 aa. Conserved hypothetical protein, similar to C-terminal part of P31511|35KD_MYCTU 35kd immunogenic protein from Mycobacterium tuberculosis (270 aa), FASTA scores: opt: 159, E(): 0.002, (50.90% identity in 55 aa overlap); and to Mycobacterium leprae ML0981 possible pseudogene, an orthologue of 35kd immunogenic protein from Mycobacterium tuberculosis. Size difference suggests possible gene fragment. L7N673 No conserved hypotheticals L7N673 MMAR_2445 MSMEG_3804 Mb1665c NC_000962.3 Mycobrowser_v4 CDS 1614329 1615567 . + 0 Rv1437 pgk Involved in the second phase of glycolysis (second step) [catalytic activity: ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate] Probable phosphoglycerate kinase Pgk Rv1437, (MTCY493.17c), len: 412 aa. Probable pgk, Phosphoglycerate kinase, highly similar to many e.g. PGK_MYCLE|P46712 Mycobacterium leprae (416 aa), FASTA scores: opt: 2153, E(): 0, (80.4% identity in 414 aa overlap). Contains PS00111 Phosphoglycerate kinase signature. Belongs to the phosphoglycerate kinase family. P9WID1 No intermediary metabolism and respiration P65700 P9WID1 GO:0005737,GO:0006096,GO:0004618,GO:0005524 2.7.2.3 P9WID1 ML0571 MMAR_2240 MSMEG_3085 Mb1472 NC_000962.3 Mycobrowser_v4 CDS 1615564 1616349 . + 0 Rv1438 tpi Plays an important role in several metabolic pathways [catalytic activity: D-glyceraldehyde 3-phosphate = glycerone phosphate] Probable triosephosphate isomerase Tpi (TIM) Rv1438, (MTCY493.16c), len: 261 aa. Probable tpi (tpiA), Triosephosphate isomerase, highly similar to many e.g. TPIS_MYCLE|P46711 Mycobacterium leprae (261 aa), FASTA scores: opt: 1456, E(): 0, (83.9% identity in 261 aa overlap). Contains PS00171 Triosephosphate isomerase active site. Belongs to the triosephosphate isomerase family. P9WG43 No intermediary metabolism and respiration 3GVG,3TA6,3TAO P66940 P9WG43 GO:0006094,GO:0006096,GO:0006098,GO:0004807,GO:0005737 5.3.1.1 P9WG43 ML0572 MMAR_2241 MSMEG_3086 Mb1473 NC_000962.3 Mycobrowser_v4 CDS 1617837 1618070 . + 0 Rv1440 secG Involved in protein export. Participates in a early event of protein translocation. Probable protein-export membrane protein (translocase subunit) SecG Rv1440, (MTCY493.14c), len: 77 aa. Probable secG, protein-export membrane protein (translocase subunit) (see citation below), similar to many e.g. P38388|SECG_MYCLE probable protein-export membrane (77 aa), FASTA scores: opt: 450, E(): 6.7e-24, (96.1% identity in 77 aa overlap). Start changed since original submission (-40 aa). Part of the prokaryotic protein translocation apparatus which comprise SECA|Rv3240c, SECD|Rv2587c, SECE|Rv0638, SECF|Rv2586c, SECG and SECY|Rv0732. P9WGN5 No cell wall and cell processes P66791 P9WGN5 GO:0016021,GO:0005886,GO:0009306,GO:0055085,GO:0015450 ML0577 MMAR_2246 MSMEG_3087 Mb1475 NC_000962.3 Mycobrowser_v4 CDS 1618209 1619684 . - 0 Rv1441c PE_PGRS26 Function unknown PE-PGRS family protein PE_PGRS26 Rv1441c, (MTCY493.13), len: 491 aa. PE_PGRS26, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002), similar to Y0DP_MYCTU|Q50615 hypothetical glycine-rich 40.8 kDa protein (498 aa), fasta scores: opt: 1625, E(): 0, (55.2% identity in 518 aa overlap). Q79FP3 No PE/PPE Q79FP3 Q79FP3 Q79FP3 MMAR_0837,MMAR_2053,MMAR_2248,MMAR_2596 Mb1476c NC_000962.3 Mycobrowser_v4 CDS 1619791 1622091 . + 0 Rv1442 bisC This enzyme may serve as a scavenger, allowing the cell to utilize biotin sulfoxide as a biotin source Probable biotin sulfoxide reductase BisC (BDS reductase) (BSO reductase) Rv1442, (MTCY493.12c), len: 766 aa. Probable bisC, Biotin sulfoxide reductase, similar to BISC_ECOLI|P20099 biotin sulfoxide reductase from Escherichia coli (739 aa), FASTA scores: opt: 1271, E():0, (40.2% identity in 744 aa overlap). O06817 No intermediary metabolism and respiration O06817 O06817 GO:0055114,GO:0016491,GO:0030151 1.-.-.- O06817 MSMEG_2738 Mb1477 NC_000962.3 Mycobrowser_v4 CDS 1622207 1622692 . - 0 Rv1443c Rv1443c Unknown Unknown protein Rv1443c, (MTCY493.11), len: 161 aa. Unknown protein. O06816 No conserved hypotheticals O06816 O06816 O06816 MMAR_0169 Mb1478c NC_000962.3 Mycobrowser_v4 CDS 1624454 1625365 . - 0 Rv1446c opcA May be involved in the functional assembly of glucose 6-phosphate dehydrogenase Putative OXPP cycle protein OpcA Rv1446c, (MTCY493.08), len: 303 aa. Putative opcA, OxPP cycle protein. Highly similar to S72774 B1496_F1_30 protein from Mycobacterium leprae (265 aa), FASTA scores: opt: 1056, E(): 0, (70.3% identity in 239 aa overlap). Also similar to OPCA_NOSS2|P48971 putative oxppcycle protein opca from Nostoc punctiforme (465 aa), fasta scores: opt: 177, E(): 7.3e-05, (23.4% identity in 321 aa overlap). Aids in G6PD activity. O06813 No intermediary metabolism and respiration O06813 O06813 ML0580,ML0580c MMAR_2251 MSMEG_3100 Mb1481c NC_000962.3 Mycobrowser_v4 CDS 1626959 1628080 . - 0 Rv1448c tal Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway [catalytic activity: sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-erythrose 4-phosphate + D-fructose 6-phosphate] Probable transaldolase Tal Rv1448c, (MTCY493.06), len: 373 aa. Probable tal, Transaldolase, highly similar to many e.g. TAL_MYCLE|P55193 transaldolase from Mycobacterium leprae (375 aa), FASTA scores: opt: 1891, E(): 0, (78.6% identity in 370 aa overlap). Belongs to the transaldolase family. P9WG33 No intermediary metabolism and respiration O06812 P9WG33 GO:0006098,GO:0004801,GO:0005737 2.2.1.2 P9WG33 ML0582,ML0582c MMAR_2253 MSMEG_3102 Mb1483c NC_000962.3 Mycobrowser_v4 CDS 1630638 1634627 . - 0 Rv1450c PE_PGRS27 Function unknown PE-PGRS family protein PE_PGRS27 Rv1450c, (MTCY493.04), len: 1329 aa. PE_PGRS27, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), similar to Y03A_MYCTU|Q10637 hypothetical glycine-rich 49.6 kDa protein (603 aa), fasta scores: opt: 2112, E(): 0, (56.5% identity in 630 aa overlap). Q79FP2 No PE/PPE Q79FP2 Q79FP2 Q79FP2 MMAR_1442,MMAR_3763 Mb1485c NC_000962.3 Mycobrowser_v4 CDS 1635029 1635955 . + 0 Rv1451 ctaB Thought to be involved in aerobic respiration. Probable cytochrome C oxidase assembly factor CtaB Rv1451, (MTCY493.03c), len: 308 aa. Probable ctaB, cytochrome C oxidase assembly factor, and integral membrane protein. Highly similar to several Mycobacterium leprae proteins e.g. Q49685 CYOE cytochrome O ubiquinol oxidase assembly factor (300 aa), FASTA scores: opt: 1636, E(): 0, (82.7% identity in 307 aa overlap); NP_301495.1|NC_002677 putative protoheme IX farnesyltransferase (321 aa); NP_301495.1|NC_002677 putative protoheme IX farnesyltransferase (321 aa). P9WFR7 No intermediary metabolism and respiration O06809 P9WFR7 GO:0016021,GO:0005886,GO:0008495,GO:0048034 2.5.1.- ML0584 MMAR_2255 MSMEG_3105 Mb1486 NC_000962.3 Mycobrowser_v4 CDS 1658980 1659354 . + 0 Rv1470 trxA Thioredoxin participates in various redox reactions through the reversible oxidation of its active center dithiol, to a disulfide, & catalyzes dithiol-disulfide exchange reactions Probable thioredoxin TrxA Rv1470, (MTV007.17), len: 124 aa. Probable trxA, thioredoxin, similar to many e.g. P12243|THI1_SYNP7 thioredoxin 1 from Synechococcus sp. (106 aa), FASTA scores: opt: 201, E(): 9.2e-08, (35.4% identity in 99 aa overlap); etc. Highly similar to downstream ORF Rv1471|trxB1 probable thioredoxin from Mycobacterium tuberculosis (123 aa), FASTA scores: opt: 402, E(): 0, (54.4% identity in 114 aa overlap). Warning: note that Rv3914|MT4033|MTV028.05|trxC can be alternatively named trxA. O53161 No intermediary metabolism and respiration O53161 O53161 GO:0055114,GO:0016491,GO:0045454 1.-.-.- O53161 MMAR_2276 Mb1505 NC_000962.3 Mycobrowser_v4 CDS 1638381 1639646 . + 0 Rv1453 Rv1453 Involved in transcriptional mechanism Possible transcriptional activator protein Rv1453, (MTCY493.01c), len: 421 aa. Possible transcriptional activator, similar to Q50018 putative transcriptional activator trx from Mycobacterium leprae (517 aa), FASTA scores: opt: 1719, E(): 0, (54.0% identity in 500 aa overlap). Also highly similar to Mycobacterium tuberculosis proteins Rv2370c, Rv1194c, Rv2242, Rv1186c, and to the further upstream ORF's Rv1429|MTCY493.25c (28.1% identity in 335 aa overlap). Start changed since first submission (-11 aa). O06807 No regulatory proteins O06807 O06807 MMAR_2258 Mb1488 NC_000962.3 Mycobrowser_v4 CDS 1639674 1640660 . - 0 Rv1454c qor Catalyzes the one electron reduction of certain quinones [catalytic activity: NADPH + quinone = NADP+ + semiquinone] Probable quinone reductase Qor (NADPH:quinone reductase) (zeta-crystallin homolog protein) Rv1454c, (MTV007.01c), len: 328 aa. Probable qor, quinone oxidoreductase, simiar to U87282|RCU87282_2 quinone oxidoreductase from Rhodobacter capsulatus (323 aa), FASTA scores: opt: 849, E(): 0, (44.7% identity in 329 aa overlap). Also similar to MTCY180.06 Hypothetical protein from Mycobacterium tuberculosis (334 aa), FASTA scores: opt: 430, E(): 2e-14, (32.3% identity in 350 aa overlap). Contains PS01162 Quinone oxidoreductase / zeta-crystallin signature. O53146 No intermediary metabolism and respiration O53146 O53146 GO:0055114,GO:0008270,GO:0003960 1.6.5.5 O53146 ML0585,ML0585c MSMEG_3106 Mb1489c NC_000962.3 Mycobrowser_v4 CDS 1641493 1642425 . - 0 Rv1456c Rv1456c Thought to be involved in active transport of antibiotic across the membrane (export): unidentified antibiotic resistance by an export mechanism. Responsible for the translocation of the substrate across the membrane. Probable unidentified antibiotic-transport integral membrane ABC transporter Rv1456c, (MTV007.03c), len: 310 aa. Possible unidentified antibiotic-transport integral membrane protein ABC transporter (see citation below), equivalent to Z99125|MLCL536.34 from Mycobacterium leprae (311 aa), FASTA scores: opt: 1607, E(): 0, (83.3% identity in 300 aa overlap). O53148 No cell wall and cell processes O53148 O53148 GO:0006461,GO:0016021 O53148 ML0587,ML0587c MMAR_2261 MSMEG_3117 Mb1491c NC_000962.3 Mycobrowser_v4 CDS 1642537 1643322 . - 0 Rv1457c Rv1457c Thought to be involved in active transport of antibiotic across the membrane (export): unidentified antibiotic resistance by an export mechanism. Responsible for the translocation of the substrate across the membrane. Probable unidentified antibiotic-transport integral membrane ABC transporter Rv1457c, (MTV007.04c), len: 261 aa. Possible unidentified antibiotic-transport integral membrane protein ABC transporter (see citation below), equivalent to Z99125|MLCL536.32 from Mycobacterium leprae (265 aa), FASTA scores: opt: 1415, E(): 0, (83.1% identity in 260 aa overlap). O86349 No cell wall and cell processes O86349 O86349 GO:0016020 ML0589,ML0589c MMAR_2262 MSMEG_3118 Mb1492c NC_000962.3 Mycobrowser_v4 CDS 1643319 1644260 . - 0 Rv1458c Rv1458c Thought to be involved in active transport of antibiotic across the membrane (export): unidentified antibiotic resistance by an export mechanism. Responsible for energy coupling to the transport system. Probable unidentified antibiotic-transport ATP-binding protein ABC transporter Rv1458c, (MTV007.05c), len: 313 aa. Possible unidentified antibiotic-transport ATP-binding protein ABC transporter (see citation below), equivalent to Z99125|MLCL536.31 from Mycobacterium leprae (315 aa), FASTA scores: opt: 1812, E(): 0, (88.0% identity in 308 aa overlap). Similar to AF027770|AF027770_7 ABC-type transporter in FxbA region in Mycobacterium smegmatis (284 aa), FASTA scores: opt: 1412, E(): 0, (85.1% identity in 248 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). O53149 No cell wall and cell processes O53149 O53149 GO:0016887,GO:0005524 O53149 ML0590,ML0590c MMAR_2263 MSMEG_3119 Mb1493c NC_000962.3 Mycobrowser_v4 CDS 2183372 2183869 . + 0 Rv1932 tpx Has antioxidant activity. Could remove peroxides or H(2)O(2) Probable thiol peroxidase Tpx Rv1932, (MTCY09F9.32c), len: 165 aa. Probable tpx (alternate gene name: cfp20), thiol peroxidase similar to TPX_ECOLI|P37901 thiol peroxidase (p20) from Escherichia coli (167 aa), fasta scores: opt: 535, E(): 7.3e-25, (52.4% identity in 164 aa overlap). There are four other related enzymes in M. tuberculosis: Rv2428, Rv2521, Rv2238c, Rv1608c. P9WG35 No virulence, detoxification, adaptation 1XVQ,1Y25 P66952 P9WG35 GO:0055114,GO:0008379,GO:0045454 1.11.1.- P9WG35 MMAR_2860 MSMEG_3479 Mb1967 NC_000962.3 Mycobrowser_v4 CDS 1653231 1653578 . + 0 Rv1466 Rv1466 Function unknown Conserved protein Rv1466, (MTV007.13), len: 115 aa. Conserved protein. Equivalent to Z99125|MLCL536.23c hypothetical protein from Mycobacterium leprae (115 aa), FASTA scores: opt: 648, E(): 0, (81.7% identity in 115 aa overlap). Similar to ORF's downstream of sigma factors in Streptococcus mutans and Streptococcus pneumoniae e.g. O06451 ORF3 downstream of RpoD (SPDNAGCPO) (109 aa). Alternative TTG start possible at 13757 then avoids overlap with MTV007.12. O53157 No conserved hypotheticals O53157 O53157 O53157 ML0598 MMAR_2271 MSMEG_3127 Mb1501 NC_000962.3 Mycobrowser_v4 CDS 1646186 1646992 . + 0 Rv1460 Rv1460 Involved in transcriptional mechanism Probable transcriptional regulatory protein Rv1460, (MTV007.07), len: 268 aa. Probable transcriptional regulatory protein. Equivalent to Z99125|MLCL536.29c hypothetical protein from Mycobacterium leprae (254 aa), FASTA scores: opt: 1273, E(): 0, (79.6% identity in 250 aa overlap). Possible helix-turn-helix motif between aa 68 - 89. Start changed since original submission. O53151 No regulatory proteins O53151 O53151 GO:0006355,GO:0003700,GO:0005622 O53151 ML0592 MMAR_2265 MSMEG_3121 Mb1495 NC_000962.3 Mycobrowser_v4 CDS 1649526 1650719 . + 0 Rv1462 Rv1462 Function unknown Conserved hypothetical protein Rv1462, (MTV007.09), len: 397 aa. Conserved hypothetical protein. Equivalent to MLCL536.27c|Z99125 hypothetical protein from Mycobacterium leprae (392 aa), FASTA scores: opt: 2059, E(): 0, (80.4% identity in 392 aa overlap). Also similar to nearby Mycobacterium tuberculosis hypothetical protein Rv1461. P9WFP5 No conserved hypotheticals O53153 P9WFP5 GO:0005515,GO:0016226 P9WFP5 ML0594 MMAR_2267 MSMEG_3123 Mb1497 NC_000962.3 Mycobrowser_v4 CDS 1650716 1651516 . + 0 Rv1463 Rv1463 Thought to be involved in active transport across the membrane. Responsible for energy coupling to the transport system. Probable conserved ATP-binding protein ABC transporter Rv1463, (MTV007.10), len: 266 aa. Probable conserved ATP-binding protein ABC transporter, equivalent to Z99125|MLCL536.26c putative ABC transporter ATP-binding protein from Mycobacterium leprae (260 aa), FASTA scores: opt: 1444, E(): 0, (86.0% identity in 267 aa overlap). Very similar to U38804|PPU38804_55 ATP-dependent transporter YCF16 from porphyra purpurea chloroplast (251 aa), FASTA scores: opt: 822, E(): 0, (52.4% identity in 248 aa overlap); and similar to others. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters). O53154 No cell wall and cell processes O53154 O53154 GO:0016887,GO:0006810,GO:0005524 O53154 ML0595 MMAR_2268 MSMEG_3124 Mb1498 NC_000962.3 Mycobrowser_v4 CDS 1655609 1656721 . - 0 Rv1468c PE_PGRS29 Function unknown PE-PGRS family protein PE_PGRS29 Rv1468c, (MTV007.15c), len: 370 aa. PE_PGRS29, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below). Q79FP0 No PE/PPE Q79FP0 Q79FP0 Q79FP0 Mb1503c NC_000962.3 Mycobrowser_v4 CDS 1669283 1670416 . + 0 Rv1479 moxR1 Involved in transcriptional mechanism Probable transcriptional regulatory protein MoxR1 Rv1479, (MTV007.26), len: 377 aa. Probable moxR1, transcriptional regulatory protein, similar to X96434|BBGIDBMOX_2 moxR regulator from Borrelia burgdorferi (329 aa), FASTA scores: opt: 850, E():0, (43.5% identity in 317 aa overlap); and P. denitrificans. Highly similar to MoxR homologs of Mycobacterium tuberculosis and Mycobacterium avium (but these both differ at C-terminus) e.g. Rv3692, Rv3164c, and AF0021|AF002133_6 Mycobacterium avium strain GIR10 (309 aa), FASTA scores: opt: 1181, E(): 0, (83.7% identity in 227 aa overlap). Also similar to O33173|AF006054 MoxR fragment from Mycobacterium tuberculosis (211 aa), FASTA scores: opt: 1305, E(): 0, (94.3% identity in 212 aa overlap). Note that previously known as moxR. Q79FN7 No regulatory proteins Q79FN7 Q79FN7 GO:0016887,GO:0005524 Q79FN7 ML1810,ML1810c MMAR_2286 MSMEG_3147 Mb1515 NC_000962.3 Mycobrowser_v4 CDS 1671377 1672384 . + 0 Rv1481 Rv1481 Unknown Probable membrane protein Rv1481, (MTCY277.02), len: 335 aa. Probable membrane protein, highly similar to YS02_MYCAV|O07395 hypothetical 36.1 kDa protein mav335 from Mycobacterium avium (335 aa), FASTA scores: opt: 1904, E(): 0, (89.0% identity in 337 aa overlap). Similar to AF116251|AF116251_1 BatA protein from Bacteroides fragilis (327 aa), FASTA scores: opt: 317, E(): 2e-12, (26.5% identity in 340 aa overlap). P9WFJ7 No cell wall and cell processes P64855 P9WFJ7 GO:0005886,GO:0016021 P9WFJ7 ML1808,ML1808c MMAR_2288 MSMEG_3149 Mb1517 NC_000962.3 Mycobrowser_v4 CDS 1697356 1697859 . + 0 Rv1507A Rv1507A Unknown Hypothetical protein Rv1507A, len: 167 aa. Hypothetical unknow protein. Shows weak similarity with C-terminus of Q9XHQ7|CDA9 cytidine deaminase 9 from Arabidopsis thaliana (Mouse-ear cress) (298 aa), FASTA scores: opt: 104, E(): 4.2, (33.6% identity in 133 aa overlap), blastp scores: Score: 77, Identities: 39/133 (29%), Positives: 62/133 (46%). L7N6B6 No unknown L7N6B6 NC_000962.3 Mycobrowser_v4 CDS 1762947 1763393 . + 0 Rv1558 Rv1558 Function unknown Conserved protein Rv1558, (MTCY48.07c), len: 148 aa. Conserved protein, similar to other Mycobacterial tuberculosis proteins e.g. P71854|MTCY03C7.09c|Rv3547 (151 aa), FASTA scores opt: 330, E(): 9.1e-17, (39.7% identity in 151 aa overlap); also Q11057|Rv1261c (149 aa), and O53328|Rv3178 (119 aa). Similar also to AF072709|AF072709_5 Hypothetical protein with a new amplifiable element AUD4 from Streptomyces lividans (149 aa), FASTA scores: opt: 695, E(): 0, (69.1% identity in 149 aa overlap). P9WP11 No conserved hypotheticals P64875 P9WP11 P9WP11 ML1208 MMAR_2373 Mb1584 NC_000962.3 Mycobrowser_v4 CDS 1660656 1662284 . + 0 Rv1473 Rv1473 Thought to be involved in active transport of macrolide across the membrane (export). Macrolide antibiotics resistance by an export mechanism. Responsible for energy coupling to the transport system. Probable macrolide-transport ATP-binding protein ABC transporter Rv1473, (MTV007.20), len: 542 aa. Possible macrolide-transport ATP-binding protein ABC transporter (see citation below), possibly in EF-3 subfamily. Similar to many ABC-transporters e.g. D90909_48|YHES_HAEIN from Synechocystis sp. strain PCC6803 (574 aa), FASTA scores: opt: 870, E(): 0, (33.3% identity in 525 aa overlap); P44808|YHES_HAEIN from Haemophilus influenzae (638 aa), FASTA scores: opt: 706, E(): 0, (33.7% identity in 517 aa overlap); etc. Contains two PS00017 ATP/GTP-binding site motif A (P-loop), and two PS00211 ABC transporter family signatures. Belongs to the ATP-binding transport protein family (ABC transporters). O53164 No cell wall and cell processes O53164 O53164 GO:0016887,GO:0005524 O53164 ML1816,ML1816c MMAR_2279 MSMEG_3140 Mb1508 NC_000962.3 Mycobrowser_v4 CDS 1663215 1666046 . - 0 Rv1475c acn Involved in tricarboxylic acid cycle [catalytic activity: citrate = cis-aconitate + H2O] Probable iron-regulated aconitate hydratase Acn (citrate hydro-lyase) (aconitase) Rv1475c, (MTV007.22c), len: 943 aa. Probable acn, iron-regulated aconitate hydratase, similar to many e.g. P70920|ACON_BRAJA aconitate hydratase from Bradyrhizobium japonicum (906 aa), FASTA scores: opt:1912, E(): 0, (54.8% identity in 958 aa overlap); closest to AF0021|AF002133_2 Mycobacterium avium strain GIR10 (961 aa), FASTA scores: opt: 5072, E(): 0, (82.8% identity in 943 aa overlap). Note aconitase has an active (4FE-4S) and an inactive (3FE-4S) forms. The active (4FE-4S) cluster is part of the catalytic site that interconverts citrate, cis-aconitase, and isocitrate. O53166 No intermediary metabolism and respiration O53166 O53166 GO:0003994,GO:0008152,GO:0051539 4.2.1.3 O53166 ML1814 MMAR_2282 MSMEG_3143 Mb1511c NC_000962.3 Mycobrowser_v4 CDS 1666204 1666764 . + 0 Rv1476 Rv1476 Unknown Possible membrane protein Rv1476, (MTV007.23), len: 186 aa. Possibly membrane protein, TMhelix 138-60. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53167 No cell wall and cell processes O53167 O53167 ML1813,ML1813c MMAR_2283 MSMEG_3144 Mb1512 NC_000962.3 Mycobrowser_v4 CDS 1747195 1747626 . + 0 Rv1546 Rv1546 Function unknown Conserved protein Rv1546, (MTCY48.19c), len: 143 aa. Conserved protein, similar to O05902|Rv0910|MTCY21C12.04 Hypothetical protein from Mycobacterium tuberculosis (144 aa), FASTA scores: E(): 5e-30, (37.3% identity in 142 aa overlap). P9WLU7 No conserved hypotheticals P64873 P9WLU7 P9WLU7 ML1206 MMAR_2368 Mb1573 NC_000962.3 Mycobrowser_v4 CDS 2140486 2140704 . + 0 Rv1893 Rv1893 Function unknown Conserved hypothetical protein Rv1893, (MTCY180.25c), len: 72 aa. Conserved hypothetical protein. Equivalent to MLCB561.11|AL049571 hypothetical protein from Mycobacterium leprae (74 aa), FASTA scores: opt: 317, E(): 4.6e-15, (69.4% identity in 72 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O07739 No conserved hypotheticals O07739 ML2022,ML2022c MMAR_2786 Mb1926 NC_000962.3 Mycobrowser_v4 CDS 1668419 1669144 . + 0 Rv1478 Rv1478 Unknown, but supposedly involved in virulence Possible invasion protein Rv1478, (MTV007.25), len: 241 aa. Possible invasion protein. Possibly exported protein, nearly identical to AF0060|AF006054_2 hypothetical invasion protein INV2 of Mycobacterium tuberculosis (240 aa), FASTA scores: opt: 1509, E(): 0, (95.0% identity in 241 aa overlap); very similar to AF0021|AF002133_5 hypothetical invasion protein INV2 from Mycobacterium avium (244 aa), FASTA scores: opt: 1269, E():0, (78.0% identity in 246 aa overlap). Also similar to Mycobacterium tuberculosis protein MTCY336.37 and weakly similar to C-terminal segment of p60 proteins of Listeria spp.e.g. Q01836|P60_LISIN protein P60 precursor (481 aa), FASTA scores: opt: 241, E():4e-07, (37.7% identity in 122 aa overlap). Highly similar to C-terminal domain of preceeding ORF Rv1477|MTV007.24 (472 aa), FASTA scores: opt: 864, E(): 0, (60.1% identity in 213 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). P9WHU5 No virulence, detoxification, adaptation 3PBI O53169 P9WHU5 P9WHU5 ML1811,ML1811c MMAR_2285 MSMEG_3146 Mb1514 NC_000962.3 Mycobrowser_v4 CDS 1670413 1671366 . + 0 Rv1480 Rv1480 Function unknown Conserved protein Rv1480, (MTV007.27,MTCY227.01), len: 317 aa. Conserved protein, last 110 aa residues correspond to first 110 aa of YS01_MYCAV|O07394 hypothetical 18.7 kDa Mycobacterium avium protein MAV169 (169 aa), FASTA scores: opt: 642, E(): 0, (84.2% identity in 114 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins Rv3163c and Rv3693. P9WLX5 No conserved hypotheticals P64853 P9WLX5 P9WLX5 ML1809,ML1809c MMAR_2287 MSMEG_3148 Mb1516 NC_000962.3 Mycobrowser_v4 CDS 1673440 1674183 . + 0 Rv1483 fabG1 Involved in the fatty acid biosynthesis pathway (first reduction step) (mycolic acid biosynthesis); reduces KASA/KASB products [catalytic activity: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH]. 3-oxoacyl-[acyl-carrier protein] reductase FabG1 (3-ketoacyl-acyl carrier protein reductase) (mycolic acid biosynthesis a protein) Rv1483, (MTCY277.04), len: 247 aa. FabG1 (alternate gene name: mabA), 3-oxoacyl-[acyl-carrier protein] reductase (see citations below), equivalent to O07399|FABG_MYCAV 3-oxoacyl-[acyl-carrier protein] reductase from Mycobacterium avium (255 aa); P71534|FABG_MYCSM 3-oxoacyl-[acyl-carrier protein] reductase from Mycobacterium smegmatis (255 aa); and NP_302228.1|NC_002677 3-oxoacyl-[ACP] reductase (aka MabA) from Mycobacterium leprae (253 aa). Also highly similar to many e.g. T36779 probable 3-oxacyl-(acyl-carrier-protein) reductase from Streptomyces coelicolor (234 aa); FABG_ECOLI|P25716|NP_415611.1|NC_000913 3-oxoacyl-[acyl-carrier-protein] reductase from Escherichia coli strain K12 (244 aa), FASTA scores: opt: 664, E(): 6.8e-35, (44.4% identity in 241 aa overlap); etc. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. P9WGT3 No lipid metabolism 1UZL,1UZM,1UZN,2NTN P0A5Y4 P9WGT3 GO:0051287,GO:0006633,GO:0055114,GO:0004316 1.1.1.100 ETH,INH P9WGT3 ML1807,ML1807c MMAR_2289 MSMEG_3150 Mb1519 NC_000962.3 Mycobrowser_v4 CDS 1760997 1761605 . + 0 Rv1556 Rv1556 Possibly involved in a transcriptional mechanism Possible regulatory protein Rv1556, (MTCY48.09c), len: 202 aa. Possible regulatory protein, similar to X86780|SHGCPIR2|g987088 orfY, regulator of antibiotic transport complexes from Streptomyces hygroscopicus (204 aa), FASTA score: opt: 251, E(): 1.7e-10, (33.8% identity in 201 aa overlap) and others. P9WMD1 No regulatory proteins P67436 P9WMD1 GO:0006350,GO:0003700,GO:0006355 P9WMD1 MMAR_1695 MSMEG_5340 Mb1581 NC_000962.3 Mycobrowser_v4 CDS 1778539 1779048 . + 0 Rv1571 Rv1571 Function unknown Conserved protein Rv1571, (MTCY336.32c), len: 169 aa. Conserved protein, similar at N-terminal region to Q49625|LEPB1170_C3_227 hypothetical protein from Mycobacterium leprae (104 aa), FASTA results: opt: 473, E(): 3.9e-24, (74.5% identity in 102 aa overlap). Identical to O06619|AF041819|AF041819_6 Mycobacterium bovis BCG (169 aa). O06619 No conserved hypotheticals O06619 GO:0003824,GO:0005622,GO:0016070 O06619 ML1219 MMAR_2386 MSMEG_3191 Mb1598 NC_000962.3 Mycobrowser_v4 CDS 1678942 1679172 . + 0 Rv1489A Rv1489A Function unknown Conserved protein Rv1489A, len: 76 aa. Conserved protein, similar to part of alpha subunit of many methylmalonyl-CoA mutases (~750 aa). Size difference suggests possible gene fragment although Mycobacterium tuberculosis has intact methylmalonyl-CoA mutase gene. P71774|MUTB_MYCTU probable methylmalonyl-CoA mutase from Mycobacterium tuberculosis (750 aa), FASTA scores: opt: 258, E(): 3.2e-10, (73.35% identity in 60 aa overlap). ORF predicted by GC plot. L7N6A8 No conserved hypotheticals Q8VK12 L7N6A8 GO:0016866,GO:0008152,GO:0031419 L7N6A8 NC_000962.3 Mycobrowser_v4 CDS 1675017 1676051 . + 0 Rv1485 hemZ Involved in protoheme biosynthesis (last step). Catalyzes the insertion of ferrous iron into protoporphyrin IX to form protoheme [catalytic activity: protoporphyrin + FE(2+) = protoheme + 2 H(+)]. Ferrochelatase HemZ (protoheme ferro-lyase) (heme synthetase) Rv1485, (MTCY277.06), len: 344 aa. HemZ, ferrochelatase (see citation below), similar to many e.g. HEMZ_BACSU|P32396 ferrochelatase from Bacillus subtilus (310 aa), FASTA scores: opt:490, E(): 2e-24, (30.2% identity in 295 aa overlap); etc. Belongs to the ferrochelatase family. P9WNE3 No intermediary metabolism and respiration P0A576 P9WNE3 GO:0005737,GO:0004325,GO:0006783,GO:0046872,GO:0051537 4.99.1.1 P9WNE3 ML1805,ML1805c MMAR_2291 MSMEG_3152 Mb1521 NC_000962.3 Mycobrowser_v4 CDS 1676017 1676883 . - 0 Rv1486c Rv1486c Function unknown Conserved hypothetical protein Rv1486c, (MTCY277.07c), len: 288 aa. Conserved hypothetical protein, highly similar to YS07_MYCAV|O07402 hypothetical 33.5 kDa protein mav321 from Mycobacterium avium (320 aa), FASTA scores: opt: 1217, E(): 0, (71.1% identity in 315 aa overlap). Weak similarity to AL079332|SCI5.07 hypothetical protein from Streptomyces coelicolor (259 aa), FASTA scores: opt: 131, E(): 0.29, (32.3% identity in 279 aa overlap). Start changed since original submission. P9WLX3 No conserved hypotheticals P9WLX3 ML1804 MMAR_2292 MSMEG_3153 Mb1522c NC_000962.3 Mycobrowser_v4 CDS 1690134 1690346 . - 0 Rv1498A Rv1498A Function unknown Conserved protein Rv1498A, len: 70 aa. Conserved protein, highly similar to other hypothetical proteins e.g. from Streptomyces coelicolor, Sinorhizobium meliloti and Pseudomonas aeruginosa. I6XY36 No conserved hypotheticals 3OQT,2YIZ,2YJ0 Q8VK10 I6XY36 I6XY36 MMAR_2306 Mb1536c NC_000962.3 Mycobrowser_v4 CDS 2176550 2176930 . - 0 Rv1924c Rv1924c Unknown Unknown protein Rv1924c, (MTCY09F9.40), len: 126 aa. Unknown protein. P95289 No conserved hypotheticals P95289 ML1981 Mb1959c NC_000962.3 Mycobrowser_v4 CDS 1684005 1686257 . + 0 Rv1493 mutB Involved in propionic acid fermentation. Catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates [catalytic activity : (R)-2-methyl-3-oxopropanoyl-CoA = succinyl- CoA.] Probable methylmalonyl-CoA mutase large subunit MutB (MCM) Rv1493, (MTCY277.15), len: 750 aa. Probable mutB, Methylmalonyl-CoA mutase large-subunit, strong similarity to e.g. MUTB_STRCM|Q05065 methylmalonyl-CoA mutase alpha-subunit from Streptomyces cinnamonensis (733 aa), FASTA scores: opt: 3562, E(): 0, (75.8% identity in 730 aa overlap). Contains PS00544 Methylmalonyl-CoA mutase signature. Belongs to the methylmalonyl-CoA mutase family. P9WJK5 No lipid metabolism 1SE5 P65487 P9WJK5 GO:0008152,GO:0046872,GO:0004494,GO:0031419 5.4.99.2 P9WJK5 ML1799,ML1799c MMAR_2303 MSMEG_3159 Mb1530 NC_000962.3 Mycobrowser_v4 CDS 1686271 1686573 . + 0 Rv1494 mazE4 Unknown Possible antitoxin MazE4 Rv1494, (MTCY277.16), len: 100 aa. Possible mazE4, antitoxin, part of toxin-antitoxin (TA) operon with Rv1495 (See Pandey and Gerdes, 2005; Zhu et al., 2006). P9WJ91 No virulence, detoxification, adaptation P9WJ91 P9WJ91 Mb1531 NC_000962.3 Mycobrowser_v4 CDS 1686884 1687888 . + 0 Rv1496 Rv1496 Possibly involved in transport (possibly arginine) Possible transport system kinase Rv1496, (MTCY277.18), len: 334 aa. Possible transport system kinase. Equivalent to NP_302220.1|NC_002677 putative kinase from Mycobacterium leprae (327 aa). Highly similar to several transport system kinases and NTPase transporters e.g. P27254|ARGK_ECOLI|B2918 LAO/AO transport system kinase from Escherichia coli K12 (331 aa) (see citation below); NP_311815.1|NC_002695 ATPase component of two convergent arginine transporter from Escherichia coli O157:H7 (331 aa); etc. Also similar to YPLE_CAUCR|P37895 hypothetical 34.6 kDa protein in Caulobacter crescentus (326 aa), FASTA scores, opt: 1125, E(): 0, (55.7% identity in 316 aa overlap). P9WPZ1 No cell wall and cell processes 3MD0,3P32 P63577 P9WPZ1 GO:0005524 3.6.-.- P9WPZ1 ML1798,ML1798c MMAR_2304 MSMEG_3160 Mb1533 NC_000962.3 Mycobrowser_v4 CDS 1687941 1689230 . + 0 Rv1497 lipL Function unknown, but supposed involvement in lipid metabolism Probable esterase LipL Rv1497, (MTCY277.19), len: 429 aa. Probable LipL, esterase, very similar to Mycobacterium tuberculosis hypothetical esterases and penicillin binding proteins e.g. Rv1923, Rv2463, Rv3775, etc. Also similar to G151214|M68491 esterase estA from Pseudomonas sp (389 aa), FASTA scores: opt: 604, E(): 1e-31, (34.4% identity in 389 aa overlap). P71778 No intermediary metabolism and respiration P71778 P71778 GO:0016787 3.1.-.- P71778 ML1797,ML1797c MMAR_2305 MSMEG_3162 Mb1534 NC_000962.3 Mycobrowser_v4 CDS 1689303 1689920 . - 0 Rv1498c Rv1498c Causes methylation Probable methyltransferase Rv1498c, (MTCY277.20c), len: 205 aa. Probable methyltransferase. Similar to G2792343|AF040571 methyltransferase from amycolatopsis mediterranei (272 aa), FASTA scores: E(): 5.1e-11, (32.3% identity in 124 aa overlap). Contains PS00017 ATP/GTP-binding site motif A. P9WLW9 No intermediary metabolism and respiration P71779 P9WLW9 GO:0008168,GO:0008152 P9WLW9 Mb1535c NC_000962.3 Mycobrowser_v4 CDS 1791570 1792235 . + 0 Rv1591 Rv1591 Function unknown Probable transmembrane protein Rv1591, (MTCY336.13c), len: 221 aa. Probable transmembrane protein, similar to Q49626|LEPB1170_C3_229|YF91_MYCLE Hypothetical Mycobacterium leprae protein (198 aa), FASTA results: opt: 802, E(): 0, (63.8% identity in 188 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WLT5 No cell wall and cell processes P0A5F3 P9WLT5 GO:0005886,GO:0016021 ML1222 MMAR_2388 MSMEG_3196 Mb1617 NC_000962.3 Mycobrowser_v4 CDS 1691890 1692711 . + 0 Rv1501 Rv1501 Function unknown Conserved hypothetical protein Rv1501, (MTCY277.23), len: 273 aa. Conserved hypothetical protein, some similarity to O06374|Rv3633|MTCY15C10.19C hypothetical protein from Mycobacterium tuberculosis, FASTA scores: E(): 3.9e-10, (27.5% identity in 280 aa overlap). P9WI91 No conserved hypotheticals P67770 P9WI91 P9WI91 MMAR_2314 Mb1539 NC_000962.3 Mycobrowser_v4 CDS 1692924 1693823 . + 0 Rv1502 Rv1502 Unknown Hypothetical protein Rv1502, (MTCY277.24), len: 299 aa. Hypothetical unknown protein. P9WLW7 No unknown P9WLW7 P9WLW7 MMAR_2318 Mb1540,Mb1541 NC_000962.3 Mycobrowser_v4 CDS 1693996 1694544 . - 0 Rv1503c Rv1503c Function unknown Conserved hypothetical protein Rv1503c, (MTCY277.25c), len: 182 aa. Conserved hypothetical protein, similar to C-terminal region of P27833|RFFA_ECOLI lipopolysaccharide biosynthesis protein from Escherichia coli (376 aa), FASTA scores: opt: 565, E(): 0, (49.4% identity in 170 aa overlap); Rv1503c and Rv1504c are both similar to RFFA_ECOLI but are separated by a stop codon, sequence appears to be correct so possible pseudogene. L0T8G4 No conserved hypotheticals Q79FN3 L0T8G4 GO:0030170,GO:0003824 L0T8G4 Mb1542c NC_000962.3 Mycobrowser_v4 CDS 1694545 1695144 . - 0 Rv1504c Rv1504c Function unknown Conserved hypothetical protein Rv1504c, (MTCY277.26c), len: 199 aa. Conserved hypothetical protein, similar to N-terminal region of P27833|RFFA_ECOLI lipopolysaccharide biosynthesis protein from Escherichia coli (376 aa), FASTA scores: opt: 863, E(): 0, (68.0% identity in 194 aa overlap); Rv1503c and Rv1504c are similar to RFFA_ECOLI but are separated by a stop codon, sequence appears to be correct so possible pseudogene. L0T6V0 No conserved hypotheticals Q79FN2 L0T6V0 GO:0030170,GO:0003824 L0T6V0 Mb1542c NC_000962.3 Mycobrowser_v4 CDS 1698095 1699894 . - 0 Rv1508c Rv1508c Unknown Probable membrane protein Rv1508c, (MTCY277.30c), len: 599 aa. Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). Probable membrane protein. P71787 No cell wall and cell processes P71787 P71787 P71787 MMAR_2327 NC_000962.3 Mycobrowser_v4 CDS 1733610 1734737 . + 0 Rv1533 Rv1533 Unknown Conserved protein Rv1533, (MTCY07A7A.02), len: 375 aa. Conserved protein. Similar to 2NPD_NEUCR|Q01284 2-nitropropane dioxygenase precursor (378 aa), fasta scores: opt: 279, E(): 9.1e-11, (31.3% identity in 256 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins Rv1894c, Rv0021c, Rv3553, Rv2781c. O06179 No conserved hypotheticals O06179 O06179 GO:0004497,GO:0055114,GO:0018580 1.13.12.- O06179 MMAR_2346 MSMEG_0992 Mb1560 NC_000962.3 Mycobrowser_v4 CDS 2218052 2218717 . + 0 Rv1975 Rv1975 Unknown Conserved hypothetical protein Rv1975, (MTV051.13), len: 221 aa. Conserved hypothetical protein, showing some similarity to AJ251435 hypothetical protein from Mycobacterium avium subsp. paratuberculosis (193 aa). Predicted to be an outer membrane protein (See Song et al., 2008). O53976 No conserved hypotheticals O53976 O53976 MMAR_2890 MSMEG_0355 NC_000962.3 Mycobrowser_v4 CDS 4095324 4095530 . - 0 Rv3656c Rv3656c Function unknown Conserved hypothetical protein Rv3656c, (MTV025.004c), len: 68 aa. Conserved hypothetical protein, similar to Q9X918|SCH5.16c small hypothetical protein from Streptomyces coelicolor (75 aa), FASTA scores: opt: 129, E(): 0.0039, (40.0% identity in 60 aa overlap). Equivalent to AAK48120 from Mycobacterium tuberculosis strain CDC1551 (42 aa) but longer 26 aa. O69624 No conserved hypotheticals O69624 MMAR_5144 Mb3680c NC_000962.3 Mycobrowser_v4 CDS 1705058 1705789 . + 0 Rv1513 Rv1513 Function unknown Conserved protein Rv1513, (MTCY277.35), len: 243 aa. Conserved protein, similar to hypothetical proteins from several organisms e.g. AJ223833|MAP223833_3 from Mycobacterium avium paratuberculosis (240 aa), FASTA scores: opt: 1053 E(): 0, (66.3% identity in 243 aa overlap); P74191|SLL1173 from Synechocystis (244 aa), FASTA scores: opt: 276, E(): 1.1e-07, (32.2 % identity in 202 aa overlap). Also highly similar to P95136|Q50460|MTCY349.33c|Rv2956 from Mycobacterium tuberculosis (243 aa), (70.0% identity in 237 aa overlap). P71792 No conserved hypotheticals P71792 P71792 NC_000962.3 Mycobrowser_v4 CDS 1705807 1706595 . - 0 Rv1514c Rv1514c Unknown Conserved hypothetical protein Rv1514c, (MTCY277.36c), len: 262 aa. Conserved hypothetical protein. Similar to other hypothetical proteins, and to WCAE_ECOLI|P71239 putative colanic acid biosynthesis glycosyl transferase (248 aa), FASTA scores: opt: 231, E(): 4.1e-08, (33.3% identity in 210 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical glycosyltransferase, Rv2957. P9WMX9 No conserved hypotheticals P71793 P9WMX9 GO:0016757 2.4.-.- P9WMX9 NC_000962.3 Mycobrowser_v4 CDS 1706630 1707526 . - 0 Rv1515c Rv1515c Function unknown Conserved hypothetical protein Rv1515c, (MTCY277.37c), len: 298 aa. Conserved hypothetical protein, similar to P71805|MTCY02B12.11C|Rv1377c Hypothetical protein from Mycobacterium tuberculosis, FASTA scores: E(): 1.3e-05, (25.4% identity in 134 aa overlap). P71794 No conserved hypotheticals P71794 P71794 GO:0008168,GO:0008152 P71794 NC_000962.3 Mycobrowser_v4 CDS 1707529 1708539 . - 0 Rv1516c Rv1516c Unknown; involved in cellular metabolism. Probable sugar transferase Rv1516c, (MTCY277.38c), len: 336 aa. Probable sugar transferase, similar to AB010970|AB010970_6 glycosyltransferase from Streptococcus mutans (465 aa), FASTA scores: opt: 388, E(): 4.1e-18, (32.7% identity in 214 aa overlap), slight similarity to SPSA_BACSU|P39621 spore coat polysaccharide biosynthesis (256 aa), fasta scores: opt: 185, E(): 6.5e-05, (26.2% identity in 187 aa overlap), strong similarity to Rv1520|MTCY19G5.08c probable sugar transferase from Mycobacterium tuberculosis (63.5% identity in 318 aa overlap). P71795 No intermediary metabolism and respiration P71795 P71795 GO:0016740 2.-.-.- P71795 NC_000962.3 Mycobrowser_v4 CDS 1708871 1709635 . + 0 Rv1517 Rv1517 Unknown Conserved hypothetical transmembrane protein Rv1517, (MTCY277.39), len: 254 aa. Conserved hypothetical transmembrane protein, similar to G466802|LEPB1170_F2_64 from Mycobacterium leprae (230 aa), FASTA scores: opt: 282, E(): 2.2e-11, (34.1% identity in 255 aa overlap). Also similar to Mycobacterium tuberculosis Rv3821|MTCY409.09c (237 aa) (36.3% identity in 256 aa overlap); and Rv3481c. P71796 No cell wall and cell processes P71796 P71796 GO:0016021 MMAR_2352 MSMEG_4733 Mb1544 NC_000962.3 Mycobrowser_v4 CDS 1709644 1710603 . + 0 Rv1518 Rv1518 Unknown, possibly glycosyl transferase Conserved hypothetical protein Rv1518, (MTCY277.40, MTCY19G5.11c), len: 319 aa. Conserved hypothetical protein, possibly glycosyl transferase involved in exopolysaccharide synthesis, similar to several hypothetical proteins and glycosyl transferases from diverse organisms e.g. P73996|D90911 from synecho cystis sp. (309 aa), Fasta scores: opt: 300, E(): 1.8e-13, (29.5% identity in 241 aa overlap). P9WLV9 No conserved hypotheticals Q50590 P9WLV9 GO:0016021 P9WLV9 Mb1545 NC_000962.3 Mycobrowser_v4 CDS 1710733 1711002 . + 0 Rv1519 Rv1519 Function unknown Conserved hypothetical protein Rv1519, (MTCY19G5.09c), len: 89 aa. Conserved hypothetical protein, high similarity to C-terminus of Q50723|MTCY78.26|Rv3402c (412 aa) (58.1% identity in 74 aa overlap). P9WLV7 No conserved hypotheticals P64861 P9WLV7 Mb1546 NC_000962.3 Mycobrowser_v4 CDS 1711028 1712068 . + 0 Rv1520 Rv1520 Unknown; thought to be involved in cellular metabolism. Probable sugar transferase Rv1520, (MTCY19G5.08c), len: 346 aa. Probable sugar transferase, similar to several e.g. AB010970|AB010970_6 Streptococcus mutans glycosyltransferase (465 aa), FASTA scores: opt: 381, E(): 1.2e-18, (31.7% identity in 240 aa overlap); O34234|Y07786 sugar transferase from Vibrio cholerae (337 aa), FASTA scores: opt: 214, E(): 8.4e-05, (25.9% identity in 212 aa overlap). Also strongly similar to Mycobacterium tuberculosis probable sugar transferase Rv1516c. Alternative nucleotide at position 1711627 (C->T; Y200Y) has been observed. P9WLV5 No intermediary metabolism and respiration P64863 P9WLV5 Mb1547 NC_000962.3 Mycobrowser_v4 CDS 1717653 1718696 . + 0 Rv1523 Rv1523 Causes methylation Probable methyltransferase Rv1523, (MTCY19G5.05c), len: 347 aa (start uncertain). Probable methyltransferase, similar to G560513|U0002O Mycobacterium leprae (270 aa), FASTA scores: opt: 965, E(): 0, (60.3% identity in 247 aa overlap). Also similar to many e.g. Q54303|X86780 methyltransferase RAPM from Streptomyces hygroscopicus (317 aa), FASTA scores: opt: 323, E(): 1e-15, (41.2% identity in 136 aa overlap). And similar to M. tuberculosis hypothetical proteins Rv2952, Rv1405c, Rv1403c, Rv0839. Q50584 No intermediary metabolism and respiration Q50584 Q50584 GO:0008168,GO:0008152 2.1.1.- Q50584 Mb1550 NC_000962.3 Mycobrowser_v4 CDS 1718726 1719970 . + 0 Rv1524 Rv1524 Unknown Probable glycosyltransferase Rv1524, (MTCY19G5.04c), len: 414 aa. Probable glycosyltransferase, similar to many e.g. P96559|U84349 glycosyltransferase GTFB from Amycolatopsis orientalis (407 aa), FASTA scores: opt: 363, E(): 6.2e-23, (28.8% identity in 430 aa overlap); also high similarity to Rv1526c|MTCY19G5.02 Mycobacterium tuberculosis hypothetical protein (58.7% identity in 416 aa overlap); and AF143772|AF143772_15 glycosyltransferase gtfB from Mycobacterium avium strain 215 (418 aa), FASTA scores: opt: 1801, E(): 0, (65.2% identity in 417 aa overlap). P9WN07 No intermediary metabolism and respiration P64865 P9WN07 GO:0030259,GO:0016758,GO:0005975 2.4.-.- P9WN07 ML2348 MMAR_2353 MSMEG_0385,MSMEG_4740 Mb1551 NC_000962.3 Mycobrowser_v4 CDS 1720017 1720802 . + 0 Rv1525 wbbL2 Possibly involved in cell wall arabinogalactan linker formation: Uses dTDP-L-rhamnose as substrate to insert the rhamnosyl residue into the cell wall. Possible rhamnosyl transferase WbbL2 Rv1525, (MT1576, MTCY19G5.03c), len: 261 aa. Possible wbbL2, rhamnosyl transferase (see citation below), showing weak similarity to several rhamnosyl transferases. Similar to AF105060|AF105060_1 Riftia pachyptila endosymbiont (746 aa), FASTA scores: opt: 183, E(): 0.00013, (35.2% identity in 105 aa overlap). P9WLV3 No conserved hypotheticals P9WLV3 P9WLV3 MMAR_2349 Mb1552 NC_000962.3 Mycobrowser_v4 CDS 1731373 1732476 . + 0 Rv1530 adh Catalyzes the reversible oxidation of ethanol to acetaldehyde with the concomitant reduction of NAD Probable alcohol dehydrogenase Adh Rv1530, (MTV045.04), len: 367 aa. Probable adh, alcohol dehydrogenase, zinc-dependent, similar to many e.g. AE0009|AE000958_23 Archaeoglobus fulgidus section 1 (402 aa), FASTA scores: opt: 423, E(): 1.8e-19, (31.7% identity in 341 aa overlap). Contains PS00059 Zinc-containing alcohol dehydrogenases signature. P9WQC3 No intermediary metabolism and respiration O53904 P9WQC3 GO:0055114,GO:0008270,GO:0004022 1.1.1.1 P9WQC3 MMAR_3163 Mb1557 NC_000962.3 Mycobrowser_v4 CDS 2218844 2219251 . - 0 Rv1976c Rv1976c Function unknown Conserved hypothetical protein Rv1976c, (MTV051.14), len: 135 aa. Conserved hypothetical protein, similar to SC1C3.03c|AL023702 hypothetical protein from Streptomyces coelicolor (125 aa), FASTA score: opt: 223, E(): 3.3e-08, (39.6% identity in 111 aa overlap). O53977 No conserved hypotheticals O53977 O53977 O53977 ML1791,ML1791c MMAR_2892 MSMEG_3467 NC_000962.3 Mycobrowser_v4 CDS 1722083 1728409 . - 0 Rv1527c pks5 Involved in polyketide metabolism. Probable polyketide synthase Pks5 Rv1527c, (MTV045.01c-MTCY19G5.01), len: 2108 aa. Probable pks5, polyketide synthase, highly similar to many e.g. MCAS_MYCBO|Q02251 mycocerosic acid synthase from Mycobacterium bovis (2110 aa), FASTA scores: opt: 6270, E(): 0, (63.6% identity in 2126 aa overlap). O53901 No lipid metabolism O53901 O53901 GO:0009058,GO:0048037,GO:0055114,GO:0016491,GO:0016740,GO:0008270,GO:0000036 O53901 MMAR_2340 MSMEG_4727 Mb1554c NC_000962.3 Mycobrowser_v4 CDS 1728953 1729450 . - 0 Rv1528c papA4 Function unknown; thought to be involved in lipid metabolism. Probable conserved polyketide synthase associated protein PapA4 Rv1528c, (MTV045.02), len: 165 aa. Probable papA4, conserved polyketide synthase (PKS) associated protein; shows some similarity to C-terminal part of hypothetical proteins from Mycobacterium tuberculosis and Mycobacterium leprae e.g. Z97188|MTCY409_10 Mycobacterium tuberculosis cosmid (468) (37.9% identity in 66 aa overlap); or U00010_11 Mycobacterium leprae cosmid B1170 (35.7% identity in 84 aa overlap). Also similar to Mycobacterium tuberculosis PKS-associated proteins Rv1182, Rv3824c, Rv3820c. O53902 No lipid metabolism O53902 Mb1555c NC_000962.3 Mycobrowser_v4 CDS 1729502 1731256 . + 0 Rv1529 fadD24 Function unknown, but involvement in lipid degradation. Probable fatty-acid-AMP ligase FadD24 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) Rv1529, (MTV045.03), len: 584 aa. Probable fadD24, fatty-acid-AMP synthetase, highly similar to many e.g. MBU75685_1|AAB52538.1|U75685 acyl-CoA synthase from Mycobacterium bovis (582 aa), FASTA score: (65.6% identity in 582 aa overlap); and many other fatty-acid-CoA synthetases from Mycobacteria e.g. fadD25|MTCY19G5_7 from Mycobacterium tuberculosis (583 aa), FASTA score: (68.7% identity in 584 aa overlap); fadD28|MTCY24G1_8 from Mycobacterium tuberculosis (580 aa), FASTA score: (66.0% identity in 582 aa overlap); NP_301232.1|NC_002677|U00010_6 from Mycobacterium leprae (372 aa), FASTA score: (57.6% identity in 342 aa overlap); FADD23|Rv3826|MTCY409.04c from Mycobacterium tuberculosis (584 aa), FASTA score: (63.2% identity in 584 aa overlap); etc. O53903 No lipid metabolism O53903 O53903 GO:0008152,GO:0016874 6.2.1.- O53903 MMAR_2341 MSMEG_4731 Mb1556 NC_000962.3 Mycobrowser_v4 CDS 1732473 1733039 . + 0 Rv1531 Rv1531 Unknown Conserved protein Rv1531, (MTV045.05), len: 188 aa. Conserved protein, similar to Rv0464c|MTV038.08c (190 aa), FASTA scores: E(): 4.8e-10, (30.9% identity in 175 aa overlap). O53905 No conserved hypotheticals O53905 O53905 O53905 MMAR_2345 Mb1558 NC_000962.3 Mycobrowser_v4 CDS 1734734 1735411 . + 0 Rv1534 Rv1534 Possibly involved in a transcriptional mechanism Probable transcriptional regulator Rv1534, (MTCY07A7A.03), len: 225 aa. Probable transcriptional regulator, similar to YCDC_ECOLI|P75899 hypothetical transcriptional regulator from Escherichia coli (212 aa), FASTA scores: opt: 166, E(): 9.8e-05, (24.2% identity in 219 aa overlap). Contains PS01081 Bacterial regulatory proteins, TetR family signature and helix turn helix motif (aa 41-62). O08377 No regulatory proteins O08377 O08377 GO:0006350,GO:0003700,GO:0006355 O08377 Mb1561 NC_000962.3 Mycobrowser_v4 CDS 1736519 1739644 . + 0 Rv1536 ileS Charging ILE tRNA [catalytic activity: ATP + L-isoleucine + tRNA(ILE) = AMP + diphosphate + L-isoleucyl-tRNA(ILE)]. Isoleucyl-tRNA synthetase IleS Rv1536, (MTCY48.29c-MTCCY07A7A.05), len: 1041 aa. ileS, Isoleucyl-tRNA synthetase , similar to several e.g. SYIC_YEAST P09436 isoleucyl-tRNA synthetase (1072 aa), FASTA scores: opt: 1447, E(): 0, (37.8% identity in 1072 aa overlap); contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Belongs to class-I aminoacyl-tRNA synthetase family. P9WFV3 No information pathways Q10765 P9WFV3 GO:0005737,GO:0004822,GO:0006428,GO:0046677,GO:0008270,GO:0005524 6.1.1.5 P9WFV3 ML1195 MMAR_2356 MSMEG_3169 Mb1563 NC_000962.3 Mycobrowser_v4 CDS 1741212 1742192 . - 0 Rv1538c ansA Conversion of asparagine to aspartate [catalytic activity: L-asparagine + H(2)O = L-aspartate + NH(3).] Probable L-aparaginase AnsA Rv1538c, (MTCY48.27), len: 326 aa. Probable ansA, L-aparaginase, most similar to ASPG_BACLI|P30363 L-asparaginase (322 aa), FASTA scores: opt: 417, E(): 8.8e-19, (30.9% identity in 314 aa overlap). Contains PS00917 Asparaginase / glutaminase active site signature 2. P9WPX5 No intermediary metabolism and respiration P63627 P9WPX5 GO:0006520,GO:0005737,GO:0004067 3.5.1.1 P9WPX5 ML1198,ML1198c MMAR_2360 MSMEG_3173 Mb1565c NC_000962.3 Mycobrowser_v4 CDS 1742244 1742852 . + 0 Rv1539 lspA This protein specifically catalyzes the removal of signal peptides from prolipoproteins [catalytic activity: cleavage of N-terminal leader sequences from membrane prolipoproteins. Hydrolyses XAA-XBB-XBB-|-CYS, in which XAA is hydrophobic (preferably LEU), XBB is often SER or ala, XCC is often GLY or ala, and the CYS is alkylated on sulfur with a diacylglyceryl group]. Probable lipoprotein signal peptidase LspA Rv1539, (MTCY48.26c), len: 202 aa. Probable lspA, lipoprotein signal peptidase (see citation below), similar to several e.g. LSPA_PSEFL|P17942 (170 aa), FASTA scores: opt: 299, E(): 2.6e-12, (38.3% identity in 167 aa overlap). Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P9WK99 No cell wall and cell processes P65262 P9WK99 GO:0016021,GO:0005886,GO:0006508,GO:0004190 3.4.23.36 P9WK99 ML1199 MMAR_2361 MSMEG_3174 Mb1566 NC_000962.3 Mycobrowser_v4 CDS 1742845 1743771 . + 0 Rv1540 Rv1540 Function unknown Conserved hypothetical protein member of yabO/yceC/yfiI family Rv1540, (MTCY48.25c), len: 308 aa. Member of the yabO/yceC/yfiI family of hypothetical proteins, similar to P44445|YFII_HAEIN hypothetical protein HI0176 from Haemophilus influenzae (324 aa), FASTA scores: opt: 437, E(): 1.2e-22, (33.2% identity in 322 aa overlap). Equivalent to AL049478|MLCL458_13 hypothetical protein from Mycobacterium leprae (308 aa), (89.3% identity in 307 aa overlap). Contains PS01129 hypothetical yabO/yceC/yfiI family signature. P9WHQ3 No conserved hypotheticals P0A5T2 P9WHQ3 GO:0009982,GO:0001522,GO:0003723 5.4.99.- P9WHQ3 ML1200 MMAR_2362 MSMEG_3175 Mb1567 NC_000962.3 Mycobrowser_v4 CDS 1745064 1746089 . + 0 Rv1543 Rv1543 Thought to reduce acyl-CoA esters of fatty acids to fatty aldehydes. Possible fatty acyl-CoA reductase Rv1543, (MTCY48.22c), len: 341 aa. Possible fatty-acyl CoA reductase, highly similar to P94129|U77680 fatty acyl-CoA reductase ACR1 from Acinetobacter calcoaceticus (295 aa), FASTA scores: opt: 899, E(): 0, (48.5% identity in 293 aa overlap). Also highly similar to acrA1|Rv3391|MTV004.49|NP_217908.1|NC_000962 fatty acyl-CoA reductase from Mycobacterium tuberculosis (650 aa). Also highly similar to many oxidoreductases short-chain family. P9WGS1 No lipid metabolism P66779 P9WGS1 GO:0055114,GO:0016491 1.-.-.- P9WGS1 ML1204 MMAR_2366 Mb1570 NC_000962.3 Mycobrowser_v4 CDS 1746094 1746897 . + 0 Rv1544 Rv1544 Function unknown, but possibly involvement in lipid metabolism. Possible ketoacyl reductase Rv1544, (MTCY48.21), len: 267 aa. Possible ketoacyl reductase, highly similar to Z97179|MLCL383_26 putative oxidoreductase from Mycobacterium leprae (268 aa), FASTA score: (43.0% identity in 270 aa overlap). Also highly similar to others e.g. T29125 ketoacyl reductase homolog from Streptomyces coelicolor (276 aa); NP_470957.1|NC_003212 protein similar to ketoacyl reductases from Listeria innocua (253 aa); HETN_ANASP|P37694 ketoacyl reductase from Anabaena sp. strain PCC 7120 (287 aa), FASTA scores: opt: 379, E(): 7.5e-18, (31.6% identity in 250 aa overlap); etc. And highly similar to many oxidoreductases short-chain family. Also highly similar to Rv2509 from Mycobacterium tuberculosis (268 aa). Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. Q10782 No lipid metabolism Q10782 Q10782 GO:0055114,GO:0016491 1.3.1.- Q10782 ML1205 MMAR_2367 Mb1571 NC_000962.3 Mycobrowser_v4 CDS 1746919 1747146 . + 0 Rv1545 Rv1545 Unknown Hypothetical protein Rv1545, (MTCY48.20), len: 75 aa. Hypothetical unknown protein. P9WLU9 No conserved hypotheticals P9WLU9 Mb1572 NC_000962.3 Mycobrowser_v4 CDS 1780199 1780699 . + 0 Rv1575 Rv1575 Unknown Probable PhiRv1 phage protein Rv1575, (MTCY336.29c), len: 166 aa. Probable phiRV1 phage protein (see citation below), showing similarity in N-terminal part to Rv1574|MTCY336.30c Probable phiRV1 phage protein (103 aa), FASTA score: opt: 375, E(): 3.8e-16, (60.2% identity in 103 aa overlap); and Rv2647 Probable phiRV2 phage protein. Start changed since first submission (+49 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L0T9U5 No insertion seqs and phages L0T9U5 Mb1601,Mb1601 NC_000962.3 Mycobrowser_v4 CDS 1755445 1757310 . + 0 Rv1551 plsB1 Thought to be involved in lipid metabolism. Possible acyltransferase PlsB1 Rv1551, (MT1601, MTCY48.14c), len: 621 aa. Possible plsB1, acyltransferase, similar to PLSB_HAEIN|P44857 glycerol-3-phosphate acyltransferase from Haemophilus influenzae (810 aa), FASTA scores: opt: 434, E(): 6.2e-22, (27.6% identity in 395 aa overlap). Also similar to Rv2482c|plsB2 Probable glycerol-3-phosphate acyltransferase from Mycobacterium tuberculosis (789 aa). P9WI59 No lipid metabolism P65734 P9WI59 GO:0008654,GO:0005886,GO:0004366 P9WI59 MMAR_2371 Mb1577 NC_000962.3 Mycobrowser_v4 CDS 1747694 1751248 . + 0 Rv1547 dnaE1 DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase [catalytic activity: N deoxynucleoside triphosphate = N diphosphate + {DNA}(N)]. Probable DNA polymerase III (alpha chain) DnaE1 (DNA nucleotidyltransferase) Rv1547, (MTCY48.18c), len: 1184 aa. Probable dnaE1, DNA polymerase III, alpha chain (see citation below), similar to many e.g. DP3A_ECOLI|P10443 dna polymerase III, alpha chain (1160 aa), FASTA scores: opt: 1789, E(): 0, (36.5% identity in 1193 aa overlap). Also similar to M. tuberculosis, DnaE2|Rv3370c. Belongs to DNA polymerase type-C family, DNAE subfamily. P9WNT7 No information pathways P63977 P9WNT7 GO:0003677,GO:0006260,GO:0003887,GO:0005737,GO:0008408 2.7.7.7 P9WNT7 ML1207 MMAR_2369 MSMEG_3178 Mb1574 NC_000962.3 Mycobrowser_v4 CDS 1753510 1754037 . + 0 Rv1549 fadD11.1 Function unknown, but involvement in lipid degradation. Possible fatty-acid-CoA ligase FadD11.1 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv1549, (MTCY48.16c), len: 175 aa. Possible fadD11.1, fatty-acid-CoA synthetase, similar to the N-terminus of many fatty-acid CoA synthetases e.g. NP_147860.1|NC_000854 long-chain-fatty-acid--CoA ligase from Aeropyrum pernix (651 aa); P31685|4CL2_SOLTU 4-coumarate--CoA ligase 2 from Solanum tuberosum (Potato) (545 aa), FASTA scores: opt: 168, E(): 4.4e-06, (30.4% identity in 112 aa overlap); etc. Possible frameshift with respect to next ORF Rv1550|MTCY48.15c but we can find no sequence error to account for this. Note that previously known as fadD11'. P9WLU5 No lipid metabolism Q10777 P9WLU5 GO:0008152,GO:0003824 P9WLU5 Mb1576 NC_000962.3 Mycobrowser_v4 CDS 1782072 1782584 . - 0 Rv1577c Rv1577c Unknown Probable PhiRv1 phage protein Rv1577c, (MTCY336.27), len: 170 aa. Probable phiRv1 phage protein (prohead protease) (see citation below). Highly similar to hypothetical protein Rv2651c|MTCY441.20c phiRV2 prohead protease, FASTA scores: E(): 0, (89.3% identity in 169 aa overlap). Some similarity to VP4_BPHK7|P49860 putative bacteriophage HK97 prohead protease (gp4) (225 aa), FASTA results: opt: 176, E(): 1.3e-05, (27.3% identity in 165 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06613 No insertion seqs and phages O06613 O06613 O06613 Mb1603c NC_000962.3 Mycobrowser_v4 CDS 1794756 1795805 . + 0 Rv1594 nadA Quinolinate biosynthesis Probable quinolinate synthetase NadA Rv1594, (MTCY336.10c), len: 349 aa. Probable nadA, quinolinate synthetase. Similar to many e.g. Q49622 NADA from Mycobacterium leprae (368 aa), FASTA results: opt: 1994, E(): 0, (84.4% identity in 352 aa overlap). P9WJK1 No intermediary metabolism and respiration P65497 P9WJK1 GO:0009435,GO:0005737,GO:0046872,GO:0008987,GO:0016740,GO:0051539 2.5.1.72 P9WJK1 ML1225 MMAR_2391 MSMEG_3199 Mb1620 NC_000962.3 Mycobrowser_v4 CDS 1759435 1760178 . + 0 Rv1553 frdB Involved in interconversion of fumarate and succinate (anaerobic respiration) [catalytic activity: succinate + acceptor = fumarate + reduced acceptor]. Probable fumarate reductase [iron-sulfur subunit] FrdB (fumarate dehydrogenase) (fumaric hydrogenase) Rv1553, (MTCY48.12c), len: 247 aa. Probable frdB, fumarate reductase, iron-sulfur subunit, highly similar to others e.g. P00364|FRDB_ECOLI fumarate reductase iron-sulfur protein from Escherichia coli strain K12 (243 aa), FASTA scores: opt: 846, E(): 0, (50.0% identity in 242 aa overlap); P20921|FRDB_PROVU fumarate reductase iron-sulfur protein from Proteus vulgaris (245 aa); G64097 fumarate reductase iron-sulfur protein from Haemophilus influenzae (276 aa); etc. Contains PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature. Note that fumarate reductase forms part of an enzyme complex containing four subunits: a flavoprotein (Rv1552|frdA), an iron-sulfur (Rv1553|frdB), and two hydrophobic anchor proteins (Rv1554|frdC and Rv1555|frdD). P9WN89 No intermediary metabolism and respiration P9WN89 GO:0051538,GO:0051539,GO:0009055,GO:0022900,GO:0046872,GO:0000104,GO:0006810,GO:0006099,GO:0051537 1.3.99.1 P9WN89 Mb1579 NC_000962.3 Mycobrowser_v4 CDS 1760175 1760555 . + 0 Rv1554 frdC Involved in interconversion of fumarate and succinate (anaerobic respiration). This hydrophobic component may be required to anchor the catalytic components of the fumarate reductase complex to the cytoplasmic membrane. Probable fumarate reductase [membrane anchor subunit] FrdC (fumarate dehydrogenase) (fumaric hydrogenase) Rv1554, (MTCY48.11c), len: 126 aa. Probable frdC, fumarate reductase, membrane-anchor subunit, highly similar to others e.g. P03805|FRDC_ECOLI fumarate reductase 15 kDa hydrophobic protein from Escherichia coli strain K12 (131 aa), FASTA scores, opt: 268, E(): 3.9e-10, (31.1% identity in 122 aa overlap); NP_458780.1|NC_003198 fumarate reductase complex subunit C; membrane anchor polypeptide from Salmonella enterica subsp. enterica serovar Typhi (131 aa); P20923|FRDC_PROVU fumarate reductase 15 kDa hydrophobic protein from Proteus vulgaris (131 aa); etc. Note that fumarate reductase forms part of an enzyme complex containing four subunits: a flavoprotein (Rv1552|frdA), an iron-sulfur (Rv1553|frdB), and two hydrophobic anchor proteins (Rv1554|frdC and Rv1555|frdD). P9WNB7 No intermediary metabolism and respiration Q10762 P9WNB7 GO:0005886,GO:0016021 P9WNB7 Mb1579 NC_000962.3 Mycobrowser_v4 CDS 1760552 1760929 . + 0 Rv1555 frdD Involved in interconversion of fumarate and succinate (anaerobic respiration). This hydrophobic component may be required to anchor the catalytic components of the fumarate reductase complex to the cytoplasmic membrane. Probable fumarate reductase [membrane anchor subunit] FrdD (fumarate dehydrogenase) (fumaric hydrogenase) Rv1555, (MTCY48.10c), len: 125 aa. Probable frdD, fumarate reductase, membrane-anchor subunit, similar to others e.g. P03806|FRDD_ECOLI fumarate reductase 13 kDa hydrophobic protein from Escherichia coli strain K12 (119 aa), FASTA scores: opt: 212, E(): 4.4e-08, (36.8% identity in 106 aa overlap); etc. Note that fumarate reductase forms part of an enzyme complex containing four subunits: a flavoprotein (Rv1552|frdA), an iron-sulfur (Rv1553|frdB), and two hydrophobic anchor proteins (Rv1554|frdC and Rv1555|frdD). P9WNB5 No intermediary metabolism and respiration P67643 P9WNB5 GO:0016021,GO:0005886,GO:0006106 P9WNB5 Mb1580 NC_000962.3 Mycobrowser_v4 CDS 1782758 1783228 . - 0 Rv1578c Rv1578c Unknown Probable PhiRv1 phage protein Rv1578c, (MTCY336.26), len: 156 aa. Probable phiRv1 phage protein (terminase) (see citation below), highly similar to Rv2652c|MTCY441.21c phiRV2 phage protein from Mycobacterium tuberculosis, FASTA scores: E(): 4.8e-22, (48.1% identity in 156 aa overlap). Also similar to X65555|ARP3COS_1 hypothetical protein (cos site) - actinophage RP3 (210 aa), FASTA scores: opt: 373, E(): 6.5e-17, (50.0% identity in 114 aa overlap). Contains MIP family signature (PS00221). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06612 No insertion seqs and phages O06612 Mb1604c NC_000962.3 Mycobrowser_v4 CDS 1763428 1764717 . + 0 Rv1559 ilvA Involved in isoleucine biosynthesis (first step). Catalyzes the formation of alpha-ketobutyrate from threonine in a two step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia [catalytic activity: L-threonine + H(2)O = 2-oxobutanoate + NH(3) + H(2)O]. Probable threonine dehydratase IlvA Rv1559, (MTCY48.06c), len: 429 aa. Probable ilvA, threonine dehydratase, biosynthetic protein, similar to several e.g. THD1_CORGL|Q04513 threonine dehydratase biosynthetic (436 aa), FASTA scores: opt: 1694, E(): 0, (61.9% identity in 415 aa overlap). Contains PS00165 Serine/threonine dehydratases pyridoxal-phosphate attachment site. P9WG95 No intermediary metabolism and respiration P66897 P9WG95 GO:0009097,GO:0030170,GO:0004794 4.3.1.19 P9WG95 ML1209 MMAR_2374 MSMEG_3183 Mb1585 NC_000962.3 Mycobrowser_v4 CDS 1767135 1769432 . - 0 Rv1563c treY Involved in trehalose biosynthesis (protective effect). Mycobacteria can produce trehalose from glucose 6-phosphate and UDP-glucose (the OtsA-OtsB pathway) from glycogen-like alpha(1-->4)-linked glucose polymers (the TreY-TreZ pathway) and from maltose (the TreS pathway). Maltooligosyltrehalose synthase TreY Rv1563c, (MTCY48.02), len: 765 aa. TreY (previously called glgY), maltooligosyl trehalose synthase, confirmed biochemically (see citation below). Strong similarity to Q44315|63343 trey maltooligosyl trehalose synthase from arthrobacter SP (775 aa), fasta scores: opt: 1953, E(): 0; (46.0% identity in 789 aa overlap). Some similarity to alpha-amylases and to MTCY48.03 (30.2% identity in 215 aa overlap). May catalyse conversion of maltodextrins to maltooligosyl trehaloses. Also similar to Mycobacterium tuberculosis glgB (Rv1326c), treZ (Rv1562c). P9WQ21 No virulence, detoxification, adaptation Q10768 P9WQ21 GO:0005975,GO:0043169,GO:0047470 5.4.99.15 P9WQ21 ML1211,ML1211c MMAR_2378 Mb1589c,Mb1590c NC_000962.3 Mycobrowser_v4 CDS 1773928 1774620 . - 0 Rv1566c Rv1566c Unknown Possible Inv protein Rv1566c, (MTCY336.37), len: 230 aa. Possible inv protein, probably exported as has QQAPV repeats at C-terminus. Similar to Q49634 inv protein from Mycobacterium leprae (246 aa), FASTA scores: opt: 957, E(): 0, (70.0% identity in 207 aa overlap); also to putative invasins 1,2 (O07390, O07391) from Mycobacterium avium. Slightly similar to C-terminus of P60_LISMO|P21171 Listeria invasion-associated protein p60 precursor. Also similar to Mycobacterium tuberculosis p60 homologues Rv1477, Rv1478, Rv0024, Rv2190c. Predicted to be an outer membrane protein (See Song et al., 2008). O06624 No virulence, detoxification, adaptation O06624 O06624 O06624 ML1214,ML1214c Mb1593c NC_000962.3 Mycobrowser_v4 CDS 1776702 1777862 . + 0 Rv1569 bioF1 Involved in biotin biosynthesis (at the first step) [catalytic activity: 6-carboxyhexanoyl-CoA + L-alanine = 8-amino-7-oxononanoate + CoA + CO2]. Probable 8-amino-7-oxononanoate synthase BioF1 (AONS) (8-amino-7-ketopelargonate synthase) (7-keto-8-amino-pelargonic acid synthetase) (7-KAP synthetase) (L-alanine--pimelyl CoA ligase) Rv1569, (MTCY336.34c), len: 386 aa. Probable bioF1, 8-amino-7-oxononanoate synthase, highly similar to BIOF_MYCLE|P45487 from Mycobacterium leprae (385 aa), FASTA results: opt: 1971, E(): 0, (80.1% identity in 381 aa overlap). Also similar to BIOF2|Rv0032|MTCY10H4.32 possible 8-amino-7-oxononanoate synthase from Mycobacterium tuberculosis (771 aa), FASTA score: E(): 5.5e-29, (37.4% identity in 393 aa overlap). Contains aminotransferases class-II pyridoxal-phosphate attachment site (PS00599). Belongs to class-II of pyridoxal-phosphate-dependent aminotransferases. P9WQ87 No intermediary metabolism and respiration P0A4X4 P9WQ87 GO:0009102,GO:0008890,GO:0030170,GO:0016769,GO:0008710 2.3.1.29,2.3.1.47 P9WQ87 ML1217 MMAR_2384 MSMEG_3189 Mb1596 NC_000962.3 Mycobrowser_v4 CDS 1777859 1778539 . + 0 Rv1570 bioD Involved in bioconversion of pimelate into dethiobiotin [catalytic activity: ATP + 7,8-diaminononanoate + CO(2) = ADP + phosphate + dethiobiotin] Dethiobiotin synthetase BioD Rv1570, (MTCY336.33c), len: 226 aa. bioD, dethiobiotin synthetase. Similar to many e.g. BIOD_MYCLE|P45486 from Mycobacterium leprae (223 aa), FASTA results: opt: 1059, E(): 0, (74.8% identity in 222 aa overlap). Belongs to the dethiobiotin synthetase family. P9WPQ5 No intermediary metabolism and respiration 3FGN,3FMF,3FMI,3FPA P9WPQ5 GO:0009102,GO:0004141,GO:0000287,GO:0005524 6.3.3.3 P9WPQ5 ML1218 MMAR_2385 MSMEG_3190 Mb1597 NC_000962.3 Mycobrowser_v4 CDS 1779930 1780241 . + 0 Rv1574 Rv1574 Unknown Probable PhiRv1 phage related protein Rv1574, (MTCY336.30), len: 103 aa. Probable phiRV1 phage related protein (see citation below); some similarity to N-terminus of Rv1575|MTCY441.17 Probable phiRV1 phage protein (166 aa), E(): 1.5e-06; and Rv2647|MTCY336.29c Probable phiRV2 phage protein, E(): 3.5e-05. Helix turn helix motif present at aa 14-35 (+3.61 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L0TA08 No insertion seqs and phages L0TA08 Mb1600 NC_000962.3 Mycobrowser_v4 CDS 1783906 1784301 . - 0 Rv1581c Rv1581c Unknown Probable PhiRv1 phage protein Rv1581c, (MTCY336.23), len: 131 aa. Probable phiRv1 phage protein (see citation below). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06609 No insertion seqs and phages O06609 O06609 Mb1607c NC_000962.3 Mycobrowser_v4 CDS 1785912 1786310 . - 0 Rv1583c Rv1583c Unknown Probable PhiRv1 phage protein Rv1583c, (MTCY336.21), len: 132 aa. Probable phiRv1 phage protein (see citation below), highly similar to Rv2656c|MTCY441.25c phiRV2 phage protein (130 aa), FASTA score: E(): 1.3e-33, (81.7% identity in 131 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLU1 No insertion seqs and phages P9WLU1 Mb1609c NC_000962.3 Mycobrowser_v4 CDS 1786584 1787099 . - 0 Rv1585c Rv1585c Unknown Possible phage PhiRv1 protein Rv1585c, (MTCY336.19), len: 171 aa. Possible phage phiRv1 protein (see Hatfull 2000). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06605 No insertion seqs and phages O06605 Mb1611c NC_000962.3 Mycobrowser_v4 CDS 1788162 1789163 . - 0 Rv1587c Rv1587c Unknown Partial REP13E12 repeat protein Rv1587c, (MTCY336.17), len: 333 aa. Partial REP13E12 repeat protein (see citation below), nearly identical (but has been interrupted by phiRv1 prophage) to Q50655|MTCY251.13c|Rv0094c hypothetical 34.6 kDa protein from M. tuberculosis (317 aa), FASTA results: opt: 1511, E(): 1.1e-84, (97.75% identity in 224 aa overlap). Codon usage suggests that translation may involve frameshifting of Rv1588c mRNA in poly_C stretch into reading frame of Rv1587c. 3' end found in Rv1572c. Length extended since first submission (+115 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06603 No insertion seqs and phages O06603 O06603 O06603 Mb1613c NC_000962.3 Mycobrowser_v4 CDS 1789168 1789836 . - 0 Rv1588c Rv1588c Unknown Partial REP13E12 repeat protein Rv1588c, (MTCY336.16), len: 222 aa. Partial REP13E12 repeat protein (see citation below), nearly identical to ORF's in other Rep13E12 repeats, including Rv0095c|MTCY251.14c|Y05E_MYCTU|Q10891 hypothetical 15.4 kd protein cy251.14 from Mycobacterium tuberculosis (136 aa), FASTA results: opt: 613, E(): 9.9e-29, (86.5% identity in 111 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLT9 No insertion seqs and phages P9WLT9 Mb0098c,Mb1614c NC_000962.3 Mycobrowser_v4 CDS 1790284 1791333 . + 0 Rv1589 bioB Involved in biotin synthesis. Probable biotin synthetase BioB Rv1589, (MTCY336.15c), len: 349 aa. Probable bioB, biotin synthetase O06601. Highly similar to BIOB_MYCLE|P46715 BioB from Mycobacterium leprae (345 aa), FASTA results: opt: 1982, E(): 0, (86.5% identity in 349 aa overlap). Identical to AF041819|AF041819_9 bioB from Mycobacterium bovis BCG (349 aa). P9WPQ7 No intermediary metabolism and respiration P0A506 P9WPQ7 GO:0051539,GO:0009102,GO:0004076,GO:0005506,GO:0051537 2.8.1.6 P9WPQ7 ML1220 MMAR_2387 MSMEG_3194 Mb1615 NC_000962.3 Mycobrowser_v4 CDS 1792400 1793740 . - 0 Rv1592c Rv1592c Function unknown Conserved hypothetical protein Rv1592c, (MTCY336.12), len: 446 aa. Conserved hypothetical protein, some similarity to Q49629|B1170_F1_46 from Mycobacterium leprae (132 aa), FASTA results: opt: 332, E(): 4.5e-14, (56.3% identity in 87 aa overlap). Nearly identical to truncated Mycobacterium bovis BCG protein (148 aa) AF041819|AF041819_11. P9WK89 No conserved hypotheticals O06598 P9WK89 GO:0004806,GO:0016042 INH P9WK89 ML1223,ML1223c MMAR_2389 MSMEG_3197 Mb1618c NC_000962.3 Mycobrowser_v4 CDS 1797388 1798245 . + 0 Rv1596 nadC de novo biosynthesis of NAD and NADP [catalytic activity: nicotinate D-ribonucleotide + diphosphate + CO(2) = pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate] Probable nicotinate-nucleotide pyrophosphatase NadC Rv1596, (MTCY336.08c), len: 285 aa. Probable nadC, nicotinate-nucleotide pyrophosphatase O06594. Similar to many e.g. ADC_MYCLE|P46714 from Mycobacterium leprae (284 aa), FASTA results: opt: 1418, E(): 0,(79.2% identity in 283 aa overlap). Belongs to the NADC/MODD family. P9WJJ7 No intermediary metabolism and respiration 1QPN,1QPO,1QPQ,1QPR O06594 P9WJJ7 GO:0004514,GO:0009435 2.4.2.19 P9WJJ7 ML1227 MMAR_2393 MSMEG_3201 Mb1622 NC_000962.3 Mycobrowser_v4 CDS 1798294 1799052 . + 0 Rv1597 Rv1597 Unknown Hypothetical protein Rv1597, (MTCY336.07c), len: 252 aa. Hypothetical unknown protein. O06593 No conserved hypotheticals O06593 O06593 GO:0008168,GO:0008152 O06593 MMAR_0673 Mb1623 NC_000962.3 Mycobrowser_v4 CDS 1799073 1799483 . - 0 Rv1598c Rv1598c Function unknown Conserved protein Rv1598c, (MTCY336.06), len: 136 aa. Conserved protein, some similarity to O06389|Rv0523c|MTCY25D10.02 from Mycobacterium tuberculosis (131 aa), FASTA scores: E(): 2.2e-09, (38.4% identity in 99 aa overlap); and P95144|MTCY359.02|Rv1871c (129 aa). O06592 No conserved hypotheticals O06592 O06592 ML1256,ML1256c MMAR_2394 MSMEG_3204 Mb1624c NC_000962.3 Mycobrowser_v4 CDS 1857731 1858537 . + 0 Rv1648 Rv1648 Unknown Probable transmembrane protein Rv1648, (MTCY06H11.13), len: 268 aa. Probable transmembrane protein, some similarity to Rv3434c|MTCY77.06C (237 aa), FASTA scores: E(): 0.00039, (31.4% identity in 194 aa overlap). P94983 No cell wall and cell processes P94983 GO:0016021 ML1400 MMAR_2455 Mb1675 NC_000962.3 Mycobrowser_v4 CDS 1944809 1945627 . + 0 Rv1718 Rv1718 Function unknown Conserved hypothetical protein Rv1718, (MTCY04C12.03), len: 272 aa. Conserved hypothetical protein, similar to O29058|AF1210|AE001021 Hypothetical protein from Archeoglobus (313 aa), FASTA scores: opt: 301, E(): 8e-23, (31.6% identity in 301 aa overlap). P71976 No conserved hypotheticals P71976 P71976 P71976 Mb1746,Mb1747 NC_000962.3 Mycobrowser_v4 CDS 1951041 1951751 . - 0 Rv1725c Rv1725c Function unknown Conserved hypothetical protein Rv1725c, (MTCY04C12.10c), len: 236 aa. Conserved hypothetical protein, similar to other hypothetical proteins from diverse organisms e.g. P70885|U44893 ORF108 from butyrivibrio fibrisolvens, (108 aa), FASTA scores: opt: 223, E(): 2e-09, (39.1% identity in 92 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical transcriptional regulator, O05774|Rv3095|YU95_MYCTU (158 aa). P71983 No conserved hypotheticals P71983 P71983 P71983 MMAR_1626 Mb1754c NC_000962.3 Mycobrowser_v4 CDS 1799583 1800899 . + 0 Rv1599 hisD Involved in histidine biosynthesis pathway (tenth step). This protein is considered as a bifunctional enzyme, possessing two active sites, one an alcohol dehydrogenase and the other an aldehyde dehydrogenase [catalytic activity: L-histidinol + 2 NAD(+) + H(2)O = L-histidine + 2 NADH]. Probable histidinol dehydrogenase HisD (HDH) Rv1599, (MTCY336.05c), len: 438 aa. Probable hisD, histidinol dehydrogenase (see citation below) O08396. Similar to many e.g. HISX_MYCSM|P28736 from Mycobacterium smegmatis (445 aa), FASTA results: opt: 2356, E(): 0, (83.1% identity in 437 aa overlap). Contains histidinol dehydrogenase signature (PS00611). P9WNW9 No intermediary metabolism and respiration P63950 P9WNW9 GO:0008270,GO:0000105,GO:0004399,GO:0055114,GO:0051287 1.1.1.23 P9WNW9 ML1257 MMAR_2395 MSMEG_3205 Mb1625 NC_000962.3 Mycobrowser_v4 CDS 1802664 1803284 . + 0 Rv1602 hisH Histidine biosynthesis pathway (fifth step). Catalyzes an amidotransferase reaction that generates imidazole-glycerol phosphate and 5-aminoimidazol-4-carboxamide ribonucleotide, which is used for purine synthesis. Probable amidotransferase HisH Rv1602, (MTCY336.02c), len: 206 aa. Probable hisH, amidotransferase. Similar to many e.g. HIS5_STRCO|P16249 from Streptomyces coelicolor (222 aa), FASTA results: opt: 872, E():0, (61.0% identity in 210 aa overlap). Contains glutamine amidotransferases class-I active site (PS00442). Belongs to the HisH family. P9WMM1 No intermediary metabolism and respiration O06589 P9WMM1 GO:0006541,GO:0000105,GO:0000107,GO:0005737 2.4.2.- P9WMM1 ML1260 MMAR_2398 MSMEG_3208 Mb1628 NC_000962.3 Mycobrowser_v4 CDS 1803294 1804031 . + 0 Rv1603 hisA Histidine biosynthesis pathway (fourth step) [catalytic activity: N-(5'-phospho-D-ribosylformimino)-5-amino-1-(5''-phosphoribosyl)-4-imidazolecarb oxamide = N-(5'-phospho-D-1'-ribulosylformimino)-5-amino-1-(5''-phosphoribosyl)-4- imidazolecarboxamide.] Probable phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase HisA Rv1603, (MTV046.01-MTCY336.01c), len: 245 aa. Probable hisA, phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase, similar to many e.g. HIS4_STRCO|P16250 phosphoribosylformimino-5-aminoimidaz from Streptomyces coelicolor (240 aa), FASTA scores: opt: 1081, E(): 0, (69.0% identity in 239 aa overlap). P9WMM5 No intermediary metabolism and respiration 2Y85,2Y88,2Y89 P60578 P9WMM5 GO:0005737,GO:0000105,GO:0004640,GO:0000162,GO:0003949 5.3.1.24,5.3.1.16 P9WMM5 ML1261 MMAR_2399 MSMEG_3209 Mb1629 NC_000962.3 Mycobrowser_v4 CDS 1804039 1804851 . + 0 Rv1604 impA Involved in inositol phosphate metabolism. It is responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides. Key enzyme of the phosphatidyl inositol signaling pathway [catalytic activity: inositol 1(or 4)-monophosphate + H(2)O = inositol + orthophosphate]. Probable inositol-monophosphatase ImpA (imp) Rv1604, (MTV046.02), len: 270 aa. Probable impA, inositol monophosphatase, similar to many e.g. AF0059|AF005905_2 inositol monophosphate phosphatase from Mycobacterium smegmatis (276 aa), FASTA scores: opt: 1241, E(): 0, (70.5% identity in 261 aa overlap). Also similar to Mycobacterium tuberculosis proteins Rv3137 and Rv2701c. O53907 No intermediary metabolism and respiration O53907 O53907 GO:0008934 3.1.3.25 O53907 ML1262 MMAR_2400 MSMEG_3210 Mb1630 NC_000962.3 Mycobrowser_v4 CDS 1804853 1805656 . + 0 Rv1605 hisF Histidine biosynthesis pathway (sixth step). Catalyzes the cyclization reaction that produces D-erythro-imidazole glycerol phosphate. Probable cyclase HisF Rv1605, (MTV046.03), len: 267 aa. Probable hisF, cyclase involved in histidine biosynthetic pathway, similar to many e.g. AF0304|AF030405_1 Corynebacterium glutamicum cyclase (257 aa), FASTA scores: opt: 1201, E(): 0, (71.9% identity in 256 aa overlap). Belongs to the HisA/HisF family. P9WMM3 No intermediary metabolism and respiration O53908 P9WMM3 GO:0000105,GO:0000107,GO:0016829,GO:0005737 4.1.3.- P9WMM3 ML1263 MMAR_2401 MSMEG_3211 Mb1631 NC_000962.3 Mycobrowser_v4 CDS 1812359 1813171 . + 0 Rv1613 trpA Tryptophan biosynthesis pathway (fifth - last step). The alpha subunit is responsible for the ALDOL cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate. [catalytic activity: L-serine + 1-(indol-3-YL)glycerol 3-phosphate = L-tryptophan + glyceraldehyde 3-phosphate + H(2)O.] Probable tryptophan synthase, alpha subunit TrpA Rv1613, (MTCY01B2.05), len: 270 aa. Probable trpA, tryptophan synthase alpha chain. FASTA best: O68906|TRPA_MYCIT tryptophan synthase alpha chain from Mycobacterium intracellulare (271 aa), opt: 1442, E(): 0, (85.3% identity in 265 aa overlap). P9WFY1 No intermediary metabolism and respiration P66980 P9WFY1 GO:0004834,GO:0000162 4.2.1.20 P9WFY1 ML1273 MMAR_2415 MSMEG_3221 Mb1639 NC_000962.3 Mycobrowser_v4 CDS 2393411 2393641 . + 0 Rv2132 Rv2132 Unknown Conserved hypothetical protein Rv2132, (MTCY270.36c), len: 76 aa. Conserved hypothetical protein. Function unknown but belongs to Mycobacterium tuberculosis protein family including Rv2871, Rv1241, Rv3321c, Rv1113, Rv0657c, Rv1560, Rv2104c, etc. Similarity to Mycobacterium tuberculosis protein Rv2871 (AL021924|MTV020_4, 84 aa). FASTA score: opt: 142, E(): 0.00036; 41.8% identity in 55 aa overlap O06243 No conserved hypotheticals O06243 O06243 GO:0006355,GO:0003677 Mb2156 NC_000962.3 Mycobrowser_v4 CDS 1807298 1807762 . - 0 Rv1608c bcpB Peroxide detoxification Probable peroxidoxin BcpB Rv1608c, (MTV046.06), len: 154 aa. Probable bcpB, peroxidoxin or bacterioferritin comigratory protein, similar to many, e.g. AE0003|ECAE000335_4 bacterioferritin comigratory protein from Escherichia coli K-12 MG1655 (156 aa), FASTA scores: opt: 329, E(): 1.2e-16, (38.2% identity in 152 aa overlap); Z97179|MLCL383_22 Mycobacterium leprae cosmid L383 (161 aa) (40.2% identity in 132 aa overlap). Also similar to Rv2428 AhpC, alkyl hydroperoxide reductase from Mycobacterium tuberculosis; and other Mycobacterium tuberculosis putative peroxidoxins Rv2521, Rv2238c, Rv1932. P9WID9 No virulence, detoxification, adaptation O53911 P9WID9 GO:0045454,GO:0016491,GO:0016209 P9WID9 ML1268,ML1268c MMAR_2410 MSMEG_3216 Mb1634c NC_000962.3 Mycobrowser_v4 CDS 1809443 1810150 . + 0 Rv1610 Rv1610 Unknown Possible conserved membrane protein Rv1610, (MTCY01B2.02), len: 235 aa. Possible conserved membrane protein. Equivalent to AL049913|MLCB1610_23 hypothetical protein from Mycobacterium leprae (264 aa), FASTA score: (65.8% identity in 231 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06128 No cell wall and cell processes O06128 O06128 ML1270 MMAR_2412 MSMEG_3218 Mb1636 NC_000962.3 Mycobrowser_v4 CDS 1810240 1811058 . + 0 Rv1611 trpC Tryptophan biosynthesis pathway (fourth step) [catalytic activity: 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-(indol-3-YL)glycerol 3-phosphate + CO(2) + H(2)O.] Probable indole-3-glycerol phosphate synthase TrpC Rv1611, (MTCY01B2.03), len: 272 aa. Probable trpC, indole-3-glycerol phosphate synthase. Similar to Q55508|SLR0546 hypothetical 33.0 kDa protein from synechocystis SP (295 aa), FASTA score: opt: 26, E(): 7.6e-32, (44.2% identity in 265 aa overlap); also similar to TRPC_AZOBR|P26938 ndole-3-glycerol-phosphate synthaseindole-3-glycerol-phosphate synthase from Azospirillum brasilense (262 aa), FASTA score: opt: 596, E(): 4.8e-30, (43.8% identity in 258 aa overlap). Equivalent to AL0499 13|MLCB1610_24 from Mycobacterium leprae (272 aa) (90.8% identity in 272 aa overlap). Contains indole-3-glycerol phosphate synthase signature (PS00614). Belongs to the TrpC family. P9WFX7 No intermediary metabolism and respiration 3QJA P0A632 P9WFX7 GO:0000162,GO:0004425 4.1.1.48 P9WFX7 ML1271 MMAR_2413 MSMEG_3219 Mb1637 NC_000962.3 Mycobrowser_v4 CDS 1830070 1830561 . - 0 Rv1628c Rv1628c Function unknown Conserved protein Rv1628c, (MTCY01B2.20c), len: 163 aa. Conserved protein, some similarity to others e.g. Q51796 ACAC protein in Pyrococcus furiosus (136 aa), FASTA scores: opt: 199, E(): 4.6e-06, (34.7% identity in 121 aa overlap). O06145 No conserved hypotheticals O06145 O06145 O06145 MMAR_2431 MSMEG_3843 Mb1654c NC_000962.3 Mycobrowser_v4 CDS 1813171 1814577 . + 0 Rv1614 lgt Prolipoprotein modification Possible prolipoprotein diacylglyceryl transferases Lgt Rv1614, (MTCY01B2.06), len: 468 aa. Possible lgt, prolipoprotein diacylglyceryl transferases, similar to many prolipoprotein diacylglyceryl transferases. FASTA scores: LGT_STAAU|P52282 prolipoprotein diacylglyceryl transferase from Staphylococcus aureus subsp. (279 aa), opt: 289, E():3.6e- 09, (31.5% identity in 257 aa overlap); AL096884|SC4G6_3 cosmid 4G6 from Streptomyces coelicolor (343 aa), opt: 735, E(): 4e-32, (46.5% identity in 391 aa overlap). P9WK93 No cell wall and cell processes O06131 P9WK93 GO:0042158,GO:0008961,GO:0005886,GO:0009249,GO:0016021 2.4.99.- P9WK93 ML1274 Mb1640 NC_000962.3 Mycobrowser_v4 CDS 1815683 1816081 . + 0 Rv1616 Rv1616 Function unknown Conserved membrane protein Rv1616, (MTCY01B2.08), len: 132 aa. Conserved membrane protein, with some similarity to other hypothetical proteins e.g. AL096884|SC4G6_9 from Streptomyces coelicolor cosmid 4G6 (148 aa), FASTA scores: opt: 245, E(): 1.7e-1 0, (36.7% identity in 128 aa overlap); Q55401|SLL0543 hypothetical 16.5 kDa protein from synechocystis SP (148 aa), FASTA scores: opt: 225, E(): 6.5e-10, (35.9% identity in 117 aa overlap). Has cysteine cluster and contains a rubredoxin signature (PS00202). O06133 No cell wall and cell processes O06133 O06133 ML1276 MMAR_2418 MSMEG_3224 Mb1642 NC_000962.3 Mycobrowser_v4 CDS 1816189 1817607 . + 0 Rv1617 pykA Produces phosphoenol pyruvate in glycolysis [catalytic activity: ATP + pyruvate = ADP + phosphoenolpyruvate] Probable pyruvate kinase PykA Rv1617, (MTCY01B2.09), len: 472 aa. Probable pykA, pyruvate kinase. FASTA best: Q46078 pyruvate kinase from corynebacterium glutamicum (475 aa), opt: 2221, E(): 0, (72.2% identity in 468 aa overlap). Belongs to the pyruvate kinase family. Phosphorylated in vitro by PknJ|Rv2088 (See Arora et al., 2010). P9WKE5 No intermediary metabolism and respiration O06134 P9WKE5 GO:0006096,GO:0000287,GO:0030955,GO:0004743,GO:0005524 2.7.1.40 P9WKE5 ML1277 MMAR_2420 MSMEG_3227 Mb1643 NC_000962.3 Mycobrowser_v4 CDS 1817615 1818517 . + 0 Rv1618 tesB1 Involved in fatty acid metabolism. Probable acyl-CoA thioesterase II TesB1 Rv1618, (MTCY01B2.10), len: 300 aa. Probable tesB1, acyl-CoA thioesterase II, similar to other acyl-CoA thioesterases e.g. TESB_ECOLI|P23911 acyl-CoA thioesterase II from Escherichia coli (285 aa), FASTA scores: opt: 495, E(): 2.9e-27, (32.5% identity in 283 aa overlap); etc. Also similar to Rv2605c|tesB2 from M. tuberculosis. O06135 No lipid metabolism O06135 O06135 GO:0016291,GO:0006637 3.1.2.- O06135 ML1278 MMAR_2421 MSMEG_3228 Mb1644 NC_000962.3 Mycobrowser_v4 CDS 1818575 1820029 . + 0 Rv1619 Rv1619 Function unknown Conserved membrane protein Rv1619, (MTCY01B2.11), len: 484 aa. Conserved membrane protein. Some similarity to N-terminus of P94974|Rv1640c|MTCY06H11.04c probable lysyl-tRNA synthetase 2 from Mycobacterium tuberculosis (1172 aa), FASTA scores: E(): 1.4e-16, (28.0% identity in 410 aa overlap); and similar in part to O69916| SC3C8.03C Putative intergral membrane protein from Streptomyces coelicolor cosmid 3C8 (589 aa), FASTA scores: opt: 453 E(): 8.4e-22, (31.3% identity in 313 aa overlap). O06136 No cell wall and cell processes O06136 O06136 ML1279 MMAR_2422 Mb1645 NC_000962.3 Mycobrowser_v4 CDS 1821690 1823273 . - 0 Rv1621c cydD Involved in active transport across the membrane of component linked with the assembly of cytochrome: involved in cytochrome biogenesis (aerobic respiration). Responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane. Probable 'component linked with the assembly of cytochrome' transport transmembrane ATP-binding protein ABC transporter CydD Rv1621c, (MTCY01B2.13c), len: 527 aa. Probable cydD, transmembrane ATP-binding protein ABC transporter involved in transport of component linked with the assembly of cytochrome (see citation below), similar to others e.g. P94366|CYDC_BACSU transport ATP-binding protein from Bacillus subtilis (567 aa), FASTA scores: opt: 784, E(): 0, (30.1% identity in 535 aa overlap); N-terminal part of AL034355|SCD78_14 from Streptomyces coelicolor (1172 aa), FASTA scores: opt: 1295, E(): 0, (44.6% identity in 534 aa overlap); etc. Also similar to Q11019|Y07D_MYCTU from Mycobacterium tuberculosis (579 aa), FASTA scores: opt: 530, E(): 6.9e-25, (29.1% identity in 530 aa overlap). Contains (PS00211) ABC transporters family signature, and (PS00017) ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters). O06138 No intermediary metabolism and respiration O06138 O06138 GO:0042626,GO:0016021,GO:0055085,GO:0005524 O06138 ML1282,ML1282c MMAR_2424 MSMEG_3231 Mb1647c NC_000962.3 Mycobrowser_v4 CDS 1823360 1824400 . - 0 Rv1622c cydB Involved in the respiratory chain (at the terminal step): aerobic respiration. Cytochrome D terminal oxidase complex is the component of the aerobic respiratory chain that is supposedly predominant when cells are grown at low aeration [catalytic activity: ubiquinol-8 + O(2) = ubiquinone-8 + H(2)O]. Probable integral membrane cytochrome D ubiquinol oxidase (subunit II) CydB (cytochrome BD-I oxidase subunit II) Rv1622c, (MTCY01B2.14c), len: 346 aa. Probable cydB, cytochrome D ubiquinol oxidase subunit II, integral membrane protein, similar to others e.g. P11027|CYDB_ECOLI cytochrome D ubiquinol oxidase subunit II from Escherichia coli strain K12 (379 aa), FASTA scores: opt: 519, E(): 0, (32.3% identity in 372 aa overlap); P94365|CYDB_BACSU cytochrome D ubiquinol oxidase subunit II from Bacillus subtilis (338 aa), FASTA scores: opt: 824, E(): 0, (39.5% identity in 337 aa overlap); etc. O06139 No intermediary metabolism and respiration O06139 O06139 GO:0055114,GO:0016491,GO:0016020 1.10.3.- ML1283,ML1283c MMAR_2425 MSMEG_3232 Mb1648c NC_000962.3 Mycobrowser_v4 CDS 1835013 1836236 . + 0 Rv1631 coaE Catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme a to form coenzyme a [catalytic activity: ATP + dephospho-CoA <=> ADP + CoA]. Probable dephospho-CoA kinase CoaE (dephosphocoenzyme a kinase) Rv1631, (MTCY01B2.23), len: 407 aa. Probable coaE, dephospho-CoA kinase, similar to many e.g. Q50178|ML1383|COAE_MYCLE dephospho-CoA kinase from Mycobacterium leprae (410 aa), FASTA scores: E(): 0, (77.5% identity in 409 aa overlap). Has ATP/GTP-binding site motif A (P-loop, PS00017) at N-terminus. In the N-terminal section; belongs to the CoaE family. In the C-terminal section; belongs to the UPF0157 (GrpB) family. P9WPA3 No intermediary metabolism and respiration P63826 P9WPA3 GO:0015937,GO:0005737,GO:0004140,GO:0005524 2.7.1.24 P9WPA3 ML1383 MMAR_2434 Mb1657 NC_000962.3 Mycobrowser_v4 CDS 1843741 1846659 . + 0 Rv1638 uvrA Involved in nucleotide excision repair. The ABC excision nuclease is a DNA repair enzyme that catalyzes the excision reaction of UV-damaged nucleotide segments producing oligomers having the modified base(S). UVRA is an ATPase and a DNA-binding protein that preferentially binds single-stranded or UV-irradiated double-stranded DNA. Probable excinuclease ABC (subunit A - DNA-binding ATPase) UvrA Rv1638, (MTCY06H11.01,MTCY06H11.02c), len: 972 aa. Probable uvrA, excinuclease ABC, subunit A; DNA-binding ATPase (see citations below), similar to many e.g. UVRA_ECOLI|P07671 excinuclease abc subunit A from Escherichia coli (940 aa), FASTA scores: opt: 2573, E(): 0, (56.2% identity in 951 aa overlap). Contains 2x PS00017 ATP/GTP-binding site motif A, PS00211 ABC transporters family signature, PS00211 ABC transporters family signature. Consists of three subunits; UVRA, UVRB and UVRC. Belongs to the ABC transporter family. UVRA subfamily. P9WQK7 No information pathways 3ZQJ P63380 P9WQK7 GO:0016887,GO:0003677,GO:0009432,GO:0005737,GO:0009381,GO:0009380,GO:0046872,GO:0006289,GO:0005524 P9WQK7 ML1392 MMAR_2443 MSMEG_3808 Mb1664 NC_000962.3 Mycobrowser_v4 CDS 1848517 1852035 . - 0 Rv1640c lysX Charging LYS tRNA [catalytic activity: ATP + L-lysine + tRNA(LYS) = AMP + diphosphate + L-lysyl-tRNA (LYS)] Lysyl-tRNA synthetase 2 LysX Rv1640c, (MTCY06H11.04c), len: 1172 aa. lysX, lysyl-tRNA synthetase 2, probable two domain protein. N-terminal part (bases 1850153 to 1852033) is similar to AL023861|SC3C8_3 hypothetical membrane protein from Streptomyces coelicolor (589 aa), Fasta scores: opt: 1426, E(): 0, (44.6% identity in 585 aa overlap). The C-terminal part is similar to SYK_CRILO|P37879 lysyl-tRNA synthetases from Cricetulus longicaudatus (Long-tailed hamster) (597 aa), Fasta scores, opt: 985, E(): 0, (36.8% identity in 524 aa overlap). Contains PS00179 Aminoacyl-transfer RNA synthetases class-II signature 1, PS00339 Aminoacyl-transfer RNA synthetases class-II signature 2. This may indicate a frame shift but sequence has been checked and no error found. Belongs to class-II aminoacyl-tRNA synthetase family. P9WFU7 No information pathways P94974 P9WFU7 GO:0005737,GO:0016021,GO:0006629,GO:0004824,GO:0006430,GO:0050071,GO:0000287,GO:0003676,GO:0009405,GO:0005886,GO:0046677,GO:0005524 2.3.2.3,6.1.1.6 P9WFU7 ML1393,ML1393c MMAR_2447 MSMEG_3796 Mb1667c NC_000962.3 Mycobrowser_v4 CDS 1825998 1826585 . - 0 Rv1624c Rv1624c Unknown Probable conserved membrane protein Rv1624c, (MTCY01B2.16c), len: 195 aa. Probable membrane protein, first start taken. Some similarity to Rv3155 nuoK, NADH dehydrogenase chain K from M. tuberculosis. Also similar to AAK72093.1|AF196488 hypothetical protein from Mycobacterium smegmatis (205 aa). Identities = 117/195 (60%). O06141 No cell wall and cell processes O06141 O06141 MMAR_2427 MSMEG_3234 Mb1650c NC_000962.3 Mycobrowser_v4 CDS 1828180 1828797 . + 0 Rv1626 Rv1626 Sensor part of a two component regulatory system Probable two-component system transcriptional regulator Rv1626, (MTCY01B2.18), len: 205 aa. Probable two-component response system transcriptional regulator, similar to many e.g. CHEY_BACSU|P24072 chemotaxis protein chey homolog (119 aa), FASTA scores: opt: 283, E(): 1.6e-16, (43.0% identity in 114 aa overlap). Also similar to AL109732|SC7H2_27 hypothetical protein from Streptomyces coelicolor (218 aa), opt: 880, E(): 0, (69.4% identity in 196 aa overlap). P9WGM3 No regulatory proteins 1S8N,1SD5 O06143 P9WGM3 GO:0031564,GO:0000156,GO:0000160,GO:0006350 P9WGM3 ML1286 MSMEG_3246 Mb1652 NC_000962.3 Mycobrowser_v4 CDS 1828865 1830073 . - 0 Rv1627c Rv1627c Thought to be involved in lipid metabolism. Probable nonspecific lipid-transfer protein Rv1627c, (MTCY01B2.19c), len: 402 aa. Probable nonspecific lipid-transfer protein, similar to many lipid carrier proteins e.g. Q51797 acetyl CoA synthase from Pyrococcus furiosus (388 aa), FASTA scores: opt: 400, E(): 3.2e-18, (34.4% identity in 407 aa overlap); etc. Also some similarity to Mycobacterium tuberculosis proteins Rv3523, Rv3540c, Rv0244, Rv2790c, Rv1323, etc. O06144 No lipid metabolism O06144 GO:0016747,GO:0008152 O06144 ML1287,ML1287c MMAR_2430 MSMEG_3844 Mb1653c NC_000962.3 Mycobrowser_v4 CDS 1846989 1848458 . - 0 Rv1639c Rv1639c Function unknown Conserved hypothetical membrane protein Rv1639c, (MTCY06H11.03c), len: 489 aa. Conserved hypothetical membrane protein. Some similarity to P35866|YLI2_CORGL Hypothetical 45.7 kDa protein from Corynebacterium glutamicum (426 aa), FASTA scores: opt: 511, E(): 2.4e-23, (28.9% identity in 370 aa overlap). Contains PS00904 protein phenyltransferases alpha subunit repeat signature P94973 No cell wall and cell processes P94973 P94973 P94973 MMAR_2446 Mb1666c NC_000962.3 Mycobrowser_v4 CDS 1854399 1855454 . - 0 Rv1645c Rv1645c Function unknown Conserved hypothetical protein Rv1645c, (MTCY06H11.10c), len: 351 aa. Conserved hypothetical protein, similar to other Mycobacterium tuberculosis hypothetical proteins e.g. O53837|Rv0826|MTV043.18 (351 aa), FASTA scores: (57.5% identity in 299 aa overlap); Q10519|Rv2237|YM37_MYCTU (255 aa), O53682|Rv0276 (306 aa). P94979 No conserved hypotheticals P94979 P94979 P94979 ML1398,ML1398c MMAR_2452 MSMEG_3781 Mb1672c NC_000962.3 Mycobrowser_v4 CDS 2201223 2201744 . - 0 Rv1954c Rv1954c Unknown Hypothetical protein Rv1954c, (MTCY09F9.10), len: 173 aa. Hypothetical unknown protein, end overlaps next ORF upstream, Rv1955 (MTCY09F9.09c). P9WLQ3 No conserved hypotheticals P9WLQ3 Mb1989c NC_000962.3 Mycobrowser_v4 CDS 1839168 1840583 . + 0 Rv1634 Rv1634 Thought to be involved in transport of drug across the membrane (export). Drug resistance by an export mechanism (confers resistance to toxic compounds by removing them for the cells). Possible drug efflux membrane protein Rv1634, (MTCY01B2.26), len: 471 aa. Possible drug efflux membrane protein of major facilitator superfamily (MFS), similar to many antibiotic resistance (efflux) proteins. FASTA best: Q56175 TU22 dTDP-glucose dehydrtatase (GRAE) from Streptomyces violaceoruber (557 aa), opt: 415, E(): 1.7e-17, (26.7% identity in 446 aa overlap). Relatives in Mycobacterium tuberculosis: MTCY369.27c, E(): 4.8e-12; MTCY20B11.14c, E(): 2.9e-10. P9WJX3 No cell wall and cell processes O06151 P9WJX3 GO:0005886,GO:0046677,GO:0055085,GO:0016021 ML1388 MMAR_2438 MSMEG_3815 Mb1660 NC_000962.3 Mycobrowser_v4 CDS 1840572 1842242 . - 0 Rv1635c Rv1635c Possibly involved in the biosynthesis of lipoarabinomannan (lam) Probable mannosyltransferase. Probable conserved transmembrane protein. Rv1635c, (MTCY01B2.27c), len: 556 aa. Probable mannosyltransferase (See Dinadayala et al., 2006). Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). Probable conserved transmembrane protein, equivalent to CAC31770.1|AL583921 Mycobacterium leprae membrane protein (527 aa), Identities = 332/527 (62%). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06152 No cell wall and cell processes O06152 GO:0016021 ML1389,ML1389c Mb1661c NC_000962.3 Mycobrowser_v4 CDS 1842451 1842891 . + 0 Rv1636 TB15.3 Function unknown Iron-regulated universal stress protein family protein TB15.3 Rv1636, (MTCY01B2.28), len: 146 aa. TB15.3, iron-regulated universal stress protein family protein (see citations below), similar to other hypothetical proteins from diverse organisms e.g. Q57951|MJ0531|Y531_METJA from Methanococcus jannaschii (170 aa), FASTA scores: opt: 188, E(): 6e-06, (32.2% identity in 149 aa overlap); also P42297|YXIE_BACSU hypothetical 15.9 kDa protein in bglh-wapa intergenic region precursor from Bacillus subtilis (148 aa), FASTA scores: opt: 162, E(): 0.00025, (30.8% identity in 156 aa overlap). Part of family of Mycobacterium tuberculosis hypothetical proteins (but lacks C-terminal region) including Rv2005c, Rv2623, Rv2026c, Rv1996, etc. P9WFC9 No virulence, detoxification, adaptation 1TQ8 O06153 P9WFC9 GO:0006950 P9WFC9 ML1390 MMAR_2440 MSMEG_3811 Mb1662 NC_000962.3 Mycobrowser_v4 CDS 1842898 1843692 . - 0 Rv1637c Rv1637c Function unknown Conserved protein Rv1637c, (MTCY01B2.29c,MTCY06H11.01c), len: 264 aa. Conserved protein, some similarity to others e.g. P05446|GLO2_RHOBL probable hydroxyacylglutathione hydrolase (255 aa), FASTA scores: opt: 252, E(): 2e-09, (39.0% identity in 146 aa overlap). Also similar to Q9Z505|AL035591|SCC54.20 putative hydrolase from Streptomyces coelicolor (218 aa), FASTA scores: opt: 732, E(): 0, (52.3% identity in 220 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins and putative glyoxylases e.g. Rv0634c, Rv3677c, Rv2581c, Rv2260. O06154 No conserved hypotheticals O06154 GO:0016787 O06154 ML1391,ML1391c MMAR_2442 MSMEG_3810 Mb1663c NC_000962.3 Mycobrowser_v4 CDS 1855764 1856696 . + 0 Rv1646 PE17 Function unknown PE family protein PE17 Rv1646, (MTCY06H11.11), len: 310 aa. PE17, Member of the Mycobacterium tuberculosis PE family of proteins (see citation below), similar to many e.g. YW36_MYCTU|Q10873 hypothetical 53.7 kd protein cy39.36c (558 aa), FASTA scores, opt: 411, E(): 1.3e-15, (34.4% identity in 320 aa overlap). L7N681 No PE/PPE Q7D879 L7N681 L7N681 MMAR_2453 Mb1673 NC_000962.3 Mycobrowser_v4 CDS 1900241 1900975 . - 0 Rv1675c cmr Involved in transcriptional mechanism Probable transcriptional regulatory protein Cmr Rv1675c, (MTV047.10c), len: 244 aa. Probable cmr, cAMP and macrophage regulator, transcriptional regulatory protein, weak similarity to D00496|LBATRP_7 trp operon from Lactobacillus casei (219 aa), FASTA scores: opt: 172, E(): 0.00011, (26.9% identity in 186 aa overlap). P9WMH5 No regulatory proteins O53922 P9WMH5 GO:0006355,GO:0003700,GO:0005622 P9WMH5 MMAR_1621 Mb1702c NC_000962.3 Mycobrowser_v4 CDS 1852928 1853122 . + 0 Rv1642 rpmI Translation 50S ribosomal protein L35 RpmI Rv1642, (MTCY06H11.06), len: 64 aa. rpmI, 50S ribosomal protein L35, similar to several e.g. RL35_SYNY3|P48959 from Synechocystis sp. (67 aa), fasta scores: opt: 179, E(): 2.7e-08, (51.6% identity in 64 aa overlap). Belongs to the L35P family of ribosomal proteins. P9WH91 No information pathways P66271 P9WH91 GO:0003735,GO:0006412,GO:0005840 P9WH91 ML1395 MMAR_2449 MSMEG_3792 Mb1669 NC_000962.3 Mycobrowser_v4 CDS 1917756 1917932 . + 0 Rv1693 Rv1693 Unknown Conserved hypothetical protein Rv1693, (MTCI125.15), len: 58 aa. Conserved hypothetical protein, shows some similarity to AL583921 hypothetical protein from Mycobacterium leprae (61 aa). Probable coiled-coil from aa 30 to 58. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O33195 No conserved hypotheticals O33195 O33195 ML1357 MMAR_2498 Mb1719 NC_000962.3 Mycobrowser_v4 CDS 1853184 1853573 . + 0 Rv1643 rplT This protein binds directly to 23S ribosomal RNA and is necessary to the in vitro assembly process of the 50S ribosomal subunit; it is not involved in the protein synthesizing functions of that subunit 50S ribosomal protein L20 RplT Rv1643, (MTCY06H11.07), len: 129 aa. rplT, 50S ribosomal protein L20, similar to several e.g. RL20_ECOLI|P02421 from Escherichia coli (117 aa), FASTA scores: opt: 438, E(): 5.8e-24, (60.3% identity in 116 aa overlap). Contains PS00937 Ribosomal protein L20 signature. P9WHC5 No information pathways P66105 P9WHC5 GO:0000027,GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WHC5 ML1396 MMAR_2450 MSMEG_3791 Mb1670 NC_000962.3 Mycobrowser_v4 CDS 1853606 1854388 . + 0 Rv1644 tsnR rRNA modification Possible 23S rRNA methyltransferase TsnR Rv1644, (MTCY06H11.08), len: 260 aa. Possible tsnR, 23S rRNA methyltransferase, similar to several e.g. TSNR_STRLU|P52393 from Streptomyces laurentii (270 aa), FASTA scores: opt: 276, E(): 3.6e-11, (27.6% identity in 261 aa overlap). Also similar to M. tuberculosis hypothetical proteins Rv0881, Rv3579c, and Rv0380c. P94978 No information pathways P94978 P94978 GO:0008173,GO:0006396,GO:0003723 2.1.1.- P94978 ML1397 MMAR_2451 MSMEG_3790 Mb1671 NC_000962.3 Mycobrowser_v4 CDS 1856774 1857724 . + 0 Rv1647 Rv1647 Thought to play an essential role in regulation of cellular metabolism by catalysing the synthesis of a second messenger, camp [catalytic activity: ATP = 3',5'-cyclic AMP + pyrophosphate]. Adenylate cyclase (ATP pyrophosphate-lyase) (adenylyl cyclase) Rv1647, (MTCY06H11.12), len: 316 aa. Adenylate cyclase, some similarity to other Mycobacterium tuberculosis proteins e.g. Q11055|Rv1264|YC64_MYCTU 42.2 kDa protein (397 aa), FASTA scores: opt: 197, E(): 9.4e-06, (27.1% identity in 181 aa overlap) and Q10400|Rv2212|YM12_MYCTU (378 aa). Belongs to adenylyl cyclase class-3 family. P94982 No intermediary metabolism and respiration P94982 P94982 GO:0016849,GO:0009190 P94982 ML1399 MMAR_2454 MSMEG_3780 Mb1674 NC_000962.3 Mycobrowser_v4 CDS 1858733 1859758 . + 0 Rv1649 pheS Charging PHE tRNA [catalytic activity: ATP + L-phenylalanine + tRNA(PHE) = AMP + diphosphate + L-phenylalanyl-tRNA(PHE)] Probable phenylalanyl-tRNA synthetase, alpha chain PheS Rv1649, (MTCY06H11.14), len: 341 aa. Probable pheS, Phenylalanyl-tRNA synthetase alpha chain, similar to several e.g. SYFA_ECOLI|P08312 from Escherichia coli (327 aa), FASTA scores: opt: 978, E(): 0, (46.5% identity in 331 aa overlap). Homology suggests this start site, but there is a potential rbs upstream of a gtg 30 bp upstream; contains PS00179 Aminoacyl-transfer RNA synthetases class-II signature 1. Belongs to class-II aminoacyl-tRNA synthetase family. PHE-tRNA synthetase alpha chain subfamily 1. P9WFU3 No information pathways P94984 P9WFU3 GO:0005737,GO:0000287,GO:0004826,GO:0006432,GO:0000049,GO:0005524 6.1.1.20 P9WFU3 ML1401 MMAR_2456 MSMEG_3778 Mb1676 NC_000962.3 Mycobrowser_v4 CDS 1869922 1870845 . + 0 Rv1656 argF Involved in arginine biosynthesis [catalytic activity: carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.] Probable ornithine carbamoyltransferase, anabolic ArgF Rv1656, (MTCY06H11.21), len: 307 aa. Probable argF, ornithine carbamoyltransferase, anabolic (see citation below), almost identical to OTCA_MYCBO|Q02095 ornithine carbamoyltransferase, anabolic from Mycobacterium bovis (307 aa), FASTA scores: opt: 1980, E(): 0, (99.0% identity in 307 aa overlap); contains PS00097 Aspartate and ornithine carbamoyltransferases signature. Belongs to the ATCases/OTCases family. P9WIT9 No intermediary metabolism and respiration 2I6U,2P2G P0A5M8 P9WIT9 GO:0006526,GO:0004585,GO:0009348,GO:0016597 2.1.3.3 P9WIT9 ML1410 MMAR_2466 MSMEG_3772 Mb1684 NC_000962.3 Mycobrowser_v4 CDS 1967705 1967917 . + 0 Rv1740 vapB34 Unknown Possible antitoxin VapB34 Rv1740, (MTCY28.02-MTCY04C12.25), len: 70 aa. Possible vapB34, antitoxin, part of toxin-antitoxin (TA) operon with Rv1741, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. P96913|Rv0623|MTCY20H10.04 (84 aa), (73.5% identity in 68 aa overlap); P71998|Rv1740 (70 aa), and O07770|Rv0608 (81 aa). P9WJ31 No virulence, detoxification, adaptation P71998 P9WJ31 P9WJ31 Mb1769 NC_000962.3 Mycobrowser_v4 CDS 1865576 1866634 . + 0 Rv1652 argC Involved in arginine biosynthesis (at the third step) [catalytic activity: N-acetyl-L-glutamate 5-semialdehyde + NADP(+) + phosphate = N-acetyl-5-glutamyl phosphate + NADPH]. Probable N-acetyl-gamma-glutamyl-phoshate reductase ArgC Rv1652, (MTCY06H11.17), len: 352 aa. Probable argC, N-acetyl-gamma-glutamyl-phosphate reductase, similar to many e.g. ARGC_STRCL|P54896 from Streptomyces clavuligerus (340 aa), FASTA scores: opt: 1119, E(): 0, (56.9% identity in 350 aa overlap); etc. Belongs to the NAGSA dehydrogenase family. P9WPZ9 No intermediary metabolism and respiration 2I3A,2I3G,2NQT P63562 P9WPZ9 GO:0051287,GO:0006526,GO:0005737,GO:0055114,GO:0046983,GO:0003942 1.2.1.38 P9WPZ9 ML1406 MMAR_2462 MSMEG_3776 Mb1680 NC_000962.3 Mycobrowser_v4 CDS 1866631 1867845 . + 0 Rv1653 argJ Arginine biosynthesis. Probable glutamate N-acetyltransferase ArgJ Rv1653, (MTCY06H11.18), len: 404 aa. Probable argJ, Glutamate n-acetyltransferase, similar to ARGJ_BACSU|P36843 from Bacillus subtilis (406 aa), fasta scores: opt: 727, E(): 0, (36.3% identity in 410 a a overlap). P9WPZ3 No intermediary metabolism and respiration 3IT4,3IT6 P63571 P9WPZ3 GO:0006526,GO:0005737,GO:0004358,GO:0004042 2.3.1.1,2.3.1.35 P9WPZ3 ML1407 MMAR_2463 MSMEG_3775 Mb1681 NC_000962.3 Mycobrowser_v4 CDS 1867842 1868726 . + 0 Rv1654 argB Arginine biosynthesis (second step) [catalytic activity: ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate] Probable acetylglutamate kinase ArgB Rv1654, (MTCY06H11.19), len: 294 aa. Probable argB, Acetylglutamate kinase, similar to ARGB_CORGL|Q59281 (294 aa), FASTA scores: opt: 1209, E(): 0, (64.4% identity in 270 aa overlap). Belongs to the acetylglutamate kinase family. P9WQ01 No intermediary metabolism and respiration 2AP9 P0A4Y6 P9WQ01 GO:0003991,GO:0006526,GO:0005737,GO:0004349,GO:0006561,GO:0005524 2.7.2.8 P9WQ01 ML1408 MMAR_2464 MSMEG_3774 Mb1682 NC_000962.3 Mycobrowser_v4 CDS 1868723 1869925 . + 0 Rv1655 argD Arginine biosynthesis (fourth step) [catalytic activity: N2-acetyl-L-ornithine + 2-oxoglutarate = N-acetyl-L-glutamate 5-semialdehyde + L-glutamate] Probable acetylornithine aminotransferase ArgD Rv1655, (MTCY06H11.20), len: 400 aa. Probable argD, Acetylornithine aminotransferase, similar to ARGD_ECOLI|P18335 (406 aa), FASTA scores: opt: 958, E(): 0, (38.6% identity in 404 aa overlap), contains PS00600 Aminotransferases class-III pyridoxal-phosphate attachment site. Belongs to class-III of pyridoxal-phosphate-dependent aminotransferases. P9WPZ7 No intermediary metabolism and respiration P63568 P9WPZ7 GO:0006526,GO:0005737,GO:0030170,GO:0003992 2.6.1.11 P9WPZ7 ML1409 MMAR_2465 MSMEG_3773 Mb1683 NC_000962.3 Mycobrowser_v4 CDS 1910776 1911399 . - 0 Rv1685c Rv1685c Function unknown Conserved hypothetical protein Rv1685c, (MTCI125.07c), len: 207 aa. Conserved hypothetical protein, some similarity to other Mycobacterium tuberculosis hypothetical regulatory proteins e.g. Q10774|Rv1556|YF56_MYCTU (202 aa), FASTA scores: opt: 111, E(): 1.7e-05, (24.1% identity in 195 aa overlap); and P95215|Rv0258c|MTCY06A4.02c (151 aa) FASTA scores: (32.9% identity in 140 aa overlap); also similar to Q9X8G9|SCE7.13C|AL049819 putative Streptomyces coelicolor transcriptional regulator (204 aa), FASTA scores: opt: 480, E(): 6.4e-25, (40.4% identity in 203 aa overlap). O33187 No conserved hypotheticals O33187 O33187 GO:0006350,GO:0003700,GO:0006355 O33187 ML1348,ML1348c MMAR_2484,MMAR_3548 MSMEG_3765 Mb1711c NC_000962.3 Mycobrowser_v4 ncRNA 1960667 1960783 . + 0 MTB000077 MTS1338 Unknown Putative small regulatory RNA MTS1338, putative small regulatory RNA (See Arnvig et al., 2011), 5'-end mapped by RLM-RACE, alternate 5'-end at position 1960601, ~100 bp band detected by Northern blot. No stable RNAs NC_000962.3 Mycobrowser_v4 CDS 1871363 1872559 . + 0 Rv1658 argG Arginine biosynthesis [catalytic activity: ATP + L-citrulline + L-aspartate = AMP + diphosphate + L-argininosuccinate] Probable argininosuccinate synthase ArgG Rv1658, (MTCY06H11.23), len: 398 aa. Probable argG, Argininosuccinate synthase, similar to ASSY_STRCL|P50986 argininosuccinate synthase from Streptomyces clavuligerus (397 aa), FASTA scores: opt: 1873, E(): 0, (67.8% identity in 397 aa overlap); contains PS00564 Argininosuccinate synthase signature 1, PS00565 Argininosuccinate synthase signature 2. Belongs to the argininosuccinate synthase family. P9WPW7 No intermediary metabolism and respiration P63642 P9WPW7 GO:0006526,GO:0004055,GO:0005737,GO:0005524 6.3.4.5 P9WPW7 ML1412 MMAR_2468 MSMEG_3770 Mb1686 NC_000962.3 Mycobrowser_v4 CDS 1872639 1874051 . + 0 Rv1659 argH Arginine biosynthesis (last step) [catalytic activity: N-(L-arginino)succinate = fumarate + L- arginine] Probable argininosuccinate lyase ArgH Rv1659, (MTCY06H11.24), len: 470 aa. Probable argH, Argininosuccinate lyase, similar to ARLY_ECOLI|P11447 argininosuccinate lyase from Escherichia coli (457 aa), FASTA scores: opt: 1091, E(): 0, (42.5% identity in 461 aa overlap); contains PS00017 ATP/GTP-binding site motif A, PS00163 Fumarate lyases signature. Belongs to the lyase 1 family. Argininosuccinate lyase subfamily. P9WPY7 No intermediary metabolism and respiration P0A4Z0 P9WPY7 GO:0004056,GO:0005737,GO:0042450 4.3.2.1 P9WPY7 ML1413 MMAR_2469 MSMEG_3769 Mb1687 NC_000962.3 Mycobrowser_v4 CDS 1874160 1875221 . + 0 Rv1660 pks10 Possibly involved in the biosynthesis of secondary metabolites [catalytic activity: 3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2] Chalcone synthase Pks10 Rv1660, (MTCY06H11.25), len: 353 aa. pks10, chalcone synthase, similar to BCSA_BACSU|P54157 putative chalcone synthase from B. subtilis (365 aa), FASTA scores: opt: 701, E(): 0, (33.1% identity in 362 aa overlap). Also similar to M. tuberculosis Rv1665|pks11 polyketide synthase (chalcone synthase); and Rv1372|pks18 polyketide synthase. Other upstream initiation sites are possible but homology suggests this start. Note pks10 has been shown to be involved in the biosynthesis of phthiocerol. P9WPF5 No lipid metabolism P94995 P9WPF5 GO:0009058,GO:0016210,GO:0008415 2.3.1.74 P9WPF5 ML1415 MMAR_2470 MSMEG_0808 Mb1688 NC_000962.3 Mycobrowser_v4 CDS 1875304 1881684 . + 0 Rv1661 pks7 Potentially involved in some intermediate steps for the synthesis of a polyketide molecule which may be involved in secondary metabolism Probable polyketide synthase Pks7 Rv1661, (MTCY06H11.26), len: 2126 aa. Probable pks7, polyketide synthase, similar to many e.g. ERY2_SACER|Q03132 erythronolide synthase, modules 3 and 4 (3567 aa), FASTA scores: E(): 0, (48.8% identity in 2131 aa overlap); also similar to Mycobacterium tuberculosis pks12. Contains PS00606 Beta-ketoacyl synthases active site, PS00012 Phosphopantetheine attachment site. P94996 No lipid metabolism P94996 P94996 GO:0009058,GO:0048037,GO:0055114,GO:0016491,GO:0031177,GO:0016740,GO:0008270,GO:0000036 P94996 ML1416 MMAR_2471 Mb1689 NC_000962.3 Mycobrowser_v4 CDS 1881704 1886512 . + 0 Rv1662 pks8 Potentially involved in some intermediate steps for the synthesis of a polyketide molecule which may be involved in secondary metabolism Probable polyketide synthase Pks8 Rv1662, (MTCY275.01-MTCY06H11.27), len: 1602 aa. Probable pks8, polyketide synthase, similar to many polyketide synthases e.g. ERY2_SACER|Q03132 erythronolide synthase, modules 3 and 4 from Saccharopolyspora erythraea (Streptomyces erythraeus) (3567 aa), FASTA scores: opt: 3319, E(): 0, (45.8% identity in 1619 aa overlap). Also similar to other Mycobacterium tuberculosis probable polyketide synthases e.g. pks7 and pks12. Contains PS00606 Beta-ketoacyl synthases active site and PS01162 Quinone oxidoreductase/zeta-crystallin signature. Note that the similarity extends into the downstream ORF Rv1663 (MTCY275.02), and this could be accounted for by a frameshift, although the sequence has been checked and no discrepancy was found. O65933 No lipid metabolism O65933 O65933 GO:0048037,GO:0055114,GO:0016491,GO:0016740,GO:0008270,GO:0009058 O65933 MMAR_2472 Mb1690 NC_000962.3 Mycobrowser_v4 CDS 2195344 2195934 . - 0 Rv1944c Rv1944c Function unknown Conserved protein Rv1944c, (MTCY09F9.20), len: 196 aa. Conserved protein, similar to C-terminal part of SCE20.29|AL136058|CAB65585.1 hypothetical protein from Streptomyces coelicolor (338 aa), blastp scores, Identities = 37/131 (28%), Positives = 51/131 (38%). P95270 No conserved hypotheticals P95270 P95270 Mb1979c NC_000962.3 Mycobrowser_v4 CDS 2200190 2200486 . - 0 Rv1951c Rv1951c Function unknown Conserved hypothetical protein Rv1951c, (MTCY09F9.13), len: 98 aa. Conserved hypothetical protein, similar to Mycobacterium tuberculosis hypothetical protein Rv2541 (135 aa) (40.9% identity in 88 aa overlap). P95263 No conserved hypotheticals P95263 Mb1986c NC_000962.3 Mycobrowser_v4 CDS 1893577 1894230 . - 0 Rv1667c Rv1667c Thought to be involved in active transport of macrolide across the membrane (export). Macrolide antibiotics resistance by an export mechanism. Responsible for energy coupling to the transport system. Probable second part of macrolide-transport ATP-binding protein ABC transporter Rv1667c, (MTV047.03c), len: 217 aa. Probable second part of macrolide-transport ATP-binding protein ABC transporter (see citation below), with similarity to C-terminal end of putative ABC transporters/ATP binding proteins, e.g. Z99108|BSUB0005_6 ABC transporter (ATP-binding protein) homolog yfmR from Bacillus subtilis (629 aa), FASTA scores: opt: 411, E(): 6.9e-17, (37.8% identity in 217 aa overlap); etc. Similarity to other NBD components of ABC transporters suggests that Rv1667c and Rv1668c should be contiguous. However, sequence has been checked and no errors found, also same sequence in M. tuberculosis CSU93 and Mycobacterium bovis. O53915 No cell wall and cell processes O53915 O53915 GO:0016887,GO:0005524 O53915 ML1239,ML1239c Mb1695c NC_000962.3 Mycobrowser_v4 CDS 1888026 1891079 . + 0 Rv1664 pks9 Potentially involved in some intermediate steps for the synthesis of a polyketide molecule which may be involved in secondary metabolism Probable polyketide synthase Pks9 Rv1664, (MTCY275.03), len: 1017 aa. Probable pks9, polyketide synthase, similar to OL56_STRAT|Q07017 oleandomycin polyketide synthase, modules 5 and 6 from Streptomyces antibioticus (3519 aa), FASTA scores: opt: 1767, E(): 0, (41.6% identity in 919 aa overlap). Similar to other Mycobacterium tuberculosis probable polyketide synthases e.g. pks6, pks8, etc. Contains PS00012 Phosphopantetheine attachment site. O06586 No lipid metabolism O06586 O06586 GO:0009058,GO:0048037,GO:0031177,GO:0016740,GO:0000036 O06586 ML1235 MMAR_2473 Mb1692 NC_000962.3 Mycobrowser_v4 CDS 1927211 1928575 . - 0 Rv1702c Rv1702c Unknown Conserved hypothetical protein Rv1702c, (MTCI125.24c), len: 454 aa. Conserved hypothetical ORF in REP13E12 degenerate repeat. Similar to other hypothetical proteins inside REP13E12 elements (often in two parts) e.g. Rv0094c|Q50655|MTCY251.13c (317 aa), FASTA scores: opt: 1284, E(): 0, (59.7% identity in 315 aa overlap); and Rv1128c, Rv1945, Rv1148c, etc. P9WLT3 No insertion seqs and phages P64885 P9WLT3 P9WLT3 MMAR_1523 MSMEG_6573 Mb1728c NC_000962.3 Mycobrowser_v4 CDS 1895725 1896087 . + 0 Rv1669 Rv1669 Unknown Hypothetical protein Rv1669, (MTV047.04B), len: 120 aa. Hypothetical unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). O86371 No conserved hypotheticals O86371 O86371 Mb1696 NC_000962.3 Mycobrowser_v4 CDS 1896120 1896467 . + 0 Rv1670 Rv1670 Function unknown Conserved hypothetical protein Rv1670, (MTV047.05), len: 115 aa. Conserved hypothetical protein, highly similar to D90908|D90908_87 Hypothetical protein of Synechocystis sp. PCC6803 complete (94 aa), FASTA scores opt: 378, E(): 3.5e-2, (55.2% identity in 96 aa overlap); also shows some similarity to Mycobacterium tuberculosis hypothetical proteins e.g. C-terminal region of O53404|Rv1056 (254 aa), and P96817|Rv0140 (126 aa). O53917 No conserved hypotheticals O53917 O53917 O53917 MMAR_0866 Mb1697 NC_000962.3 Mycobrowser_v4 CDS 1898300 1899232 . - 0 Rv1673c Rv1673c Function unknown Conserved hypothetical protein Rv1673c, (MTV047.08c), len: 310 aa. Conserved hypothetical protein, shows weak similarity to P44103|YA48_HAEIN Hypothetical protein HI10 48 precursor (369 aa), FASTA scores: E(): 8.3e-11, (26.1% identity in 330 aa overlap). O53920 No conserved hypotheticals O53920 O53920 O53920 Mb1700c NC_000962.3 Mycobrowser_v4 CDS 1899260 1899916 . - 0 Rv1674c Rv1674c Involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv1674c, (MTV047.09c), len: 218 aa. Probable transcriptional regulatory protein. Highly similar to AJ005575|SPE005575_2 Streptomyces peucetius (226 aa), FASTA scores: opt: 662, E(): 0, (50.0% identity in 208 aa overlap). Similar to Rv0324|Z96800|MTCY63.29 M. tuberculosis cosmid (226 aa), FASTA scores: opt: 579, E(): 0, (45.3% identity in 214 aa overlap). N-terminus is similar to transcriptional activators e.g. MERR_STRLI|P30346 probable mercury resistance operon regulator (125 aa), FASTA scores: opt: 183, E(): 1.9e-06, (35.6% identity in 90 aa overlap). Contains PS00380 Rhodanese signature 1. O53921 No regulatory proteins O53921 O53921 GO:0006355,GO:0008272,GO:0004792,GO:0006350,GO:0003700,GO:0005622 O53921 Mb1701c NC_000962.3 Mycobrowser_v4 CDS 1901748 1902296 . + 0 Rv1677 dsbF Unknown; possibly involved in thiol:disulfide interchange. Probable conserved lipoprotein DsbF Rv1677, (MTV047.12), len: 182 aa. Probable dsbF, conserved lipoprotein possibly involved in thiol:disulfide interchange. Highly similar to C-terminus of Z74024|MTCY274.09 mpt53 soluble secreted antigen precursor from Mycobacterium tuberculosis (173 aa), FASTA scores: opt: 482, E(): 3.6e-23, (52.8% identity in 142 aa overlap) . Also some similarity to P52237|TIPB_PSEFL thiol:disulfide interchange protein TIPB precursor from Pseudomonas fluorescens (178 aa), FASTA scores: opt: 190, E(): 4.4e-05, (28.5% identity in 151 aa overlap); and P33926|DSBE_ECOLI thiol:disulfide interchange protein from Escherichia coli (185 aa), FASTA scores: opt: 194, E(): 2.6e-05, (29.1% identity in 175 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site and PS00194 Thioredoxin family active site. Nucleotide position 1901816 in the genome sequence has been corrected, A:G resulting in Q23Q. I6XYM2 No cell wall and cell processes 1ZZO,3IOS O53924 I6XYM2 GO:0016491,GO:0045454 I6XYM2 MMAR_5057 Mb1704 NC_000962.3 Mycobrowser_v4 CDS 1902397 1903299 . + 0 Rv1678 Rv1678 Unknown Probable integral membrane protein Rv1678, (MTV047.13), len: 300 aa. Probable integral membrane protein. O53925 No cell wall and cell processes O53925 Mb1705 NC_000962.3 Mycobrowser_v4 CDS 1904429 1905253 . + 0 Rv1680 Rv1680 Unknown Hypothetical protein Rv1680, (MTCI125.02), len: 274 aa. Hypothetical unknown protein. O33182 No conserved hypotheticals O33182 O33182 Mb1707 NC_000962.3 Mycobrowser_v4 CDS 1905250 1906242 . + 0 Rv1681 moeX Involved in molybdoptenum cofactor biosynthesis Possible molybdopterin biosynthesis protein MoeX Rv1681, (MTCI125.03), len: 330 aa. Possible moeX, Molybdopterin biosynthesis protein, has weak similarity to MOAA_ECOLI|P30745 molybdenum cofactor biosynthesis protein (329 aa), FASTA scores: opt: 162, E(): 0.00081, (27.7% identity in 224 aa overlap) and to Rv3109|MTCY164.19 MoaA from Mycobacterium tuberculosis (28.5% identity in 165 aa overlap). O33183 No intermediary metabolism and respiration O33183 O33183 GO:0051536,GO:0003824 O33183 Mb1708 NC_000962.3 Mycobrowser_v4 CDS 1906403 1907320 . + 0 Rv1682 Rv1682 Function unknown Probable coiled-coil structural protein Rv1682, (MTCI125.04), len: 305 aa. Probable coiled-coil structural protein, weakly similar to many paramyosins, kinesins and plectins e.g. MYSP_ONCVO|Q02171 paramyosin from onchocerca volvulus (879 aa), fasta scores: opt: 180, E():2.6e-08, (24.4% identity in 234 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical coiled-coil proteins (wag31 antigen 84) Rv2145c and Rv2927c. O33184 No conserved hypotheticals O33184 O33184 Mb1709 NC_000962.3 Mycobrowser_v4 CDS 1910586 1910810 . + 0 Rv1684 Rv1684 Function unknown Conserved hypothetical protein Rv1684, (MTCI125.06), len: 74 aa. Conserved hypothetical protein, similar to P75844|YCAR_ECOLI Protein YCAR from Escherichia coli (60 aa), FASTA scores: opt: 108, E(): 0.00022, (39.0% identity in 59 aa overlap). O33186 No conserved hypotheticals O33186 O33186 O33186 ML1347 MMAR_2479 MSMEG_3764 NC_000962.3 Mycobrowser_v4 CDS 1911401 1912081 . - 0 Rv1686c Rv1686c Thought to be involved in active transport of undetermined substrate (possibly drug) across the membrane. Responsible for the translocation of the substrate across the membrane. Probable conserved integral membrane protein ABC transporter Rv1686c, (MTCI125.08c), len: 226 aa. Probable conserved integral membrane protein ABC transporter (see citation below), similar to AL049819|SCE7.05 putative integral membrane protein from Streptomyces coelicolor (266 aa), FASTA sacores: opt: 661, E(): 0, (45.1% identity in 226 aa overlap); and Q53627|U43537 membrane protein involved in mithramycin resistance from streptomyces argillaceus (233 aa), FASTA scores: opt: 222, E(): 5.4e-10, (28.7% identity in 216 aa overlap). O33188 No cell wall and cell processes O33188 O33188 GO:0042626,GO:0016020,GO:0006810,GO:0005524 O33188 ML1349,ML1349c MMAR_2485 MSMEG_3763 Mb1712c NC_000962.3 Mycobrowser_v4 CDS 2002626 2003870 . + 0 Rv1769 Rv1769 Function unknown Conserved protein Rv1769, (MTCY28.35), len: 414 aa. Conserved protein, similar to O88066|SCI35.31|AL031541 hypothetical protein from Streptomyces coelicolor (402 aa), FASTA scores: opt: 1341, E(): 0, (53.8% identity in 398 aa overlap). O06802 No conserved hypotheticals O06802 O06802 ML1377 MMAR_2646 Mb1798 NC_000962.3 Mycobrowser_v4 ncRNA 1914962 1915190 . - 0 MTB000054 G2 Unknown Putative small regulatory RNA G2, putative small regulatory RNA (See Arnvig and Young, 2009). Alternate 5'-end at position 1915028. Alternate 3'-end at position 1914977. No stable RNAs NC_000962.3 Mycobrowser_v4 CDS 1912979 1913590 . + 0 Rv1688 mpg Thought to be involved in base excision repair. Possible 3-methyladenine DNA glycosylase Mpg Rv1688, (MTCI125.10), len: 203 aa. Possible mpg, 3-methyladenine DNA glycosylase (see citation below), similar to several eukaryotic 3-methylpurine DNA glycosylases and 3-methyladenine DNA glycosylases e.g. Q39147|X76169 3-methyladenine glycosylase from Arabidobsis thaliana (254 aa), FASTA scores: opt: 297, E(): 8.3e-15, (31.8% identity in 198 aa overlap) and P29372|3MG_HUMAN dna-3-methyladenine glycosidase (298 aa), FASTA scores: opt: 220, E(): 7.2e-05, (36.4% identity in 184 aa overlap). Belongs to the mpg family of DNA glycosylases. P9WJP7 No information pathways P65412 P9WJP7 GO:0003905,GO:0006284,GO:0003677 3.2.2.- P9WJP7 ML1351 MMAR_2487 MSMEG_3759 Mb1714 NC_000962.3 Mycobrowser_v4 CDS 1913602 1914876 . + 0 Rv1689 tyrS Involved in translation mechanism [catalytic activity: ATP + L-tyrosine + tRNA(TYR) = AMP + diphosphate + L-tyrosyl-tRNA(TYR)] Probable tyrosyl-tRNA synthase TyrS (TYRRS) Rv1689, (MTCI125.11), len: 424 aa. Probable tyrS, Tyrosyl-tRNA synthase, highly similar to many e.g. SYY_ECOLI|P00951 Escherichia coli (423 aa), FASTA scores: opt: 1271, E(): 0, (47.3% identity in 419 aa overlap). Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Belongs to class-I aminoacyl-tRNA synthetase family. P9WFT1 No information pathways 2JAN P67611 P9WFT1 GO:0003723,GO:0005737,GO:0004831,GO:0006437,GO:0005524 6.1.1.1 P9WFT1 ML1352 MMAR_2488 MSMEG_3758 Mb1715 NC_000962.3 Mycobrowser_v4 CDS 1915527 1915910 . + 0 Rv1690 lprJ Unknown Probable lipoprotein LprJ Rv1690, (MTCI125.12), len: 127 aa. Probable lprJ, lipoprotein; contains possible signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Weakly similar to other Mycobacterium tuberculosis hypothetical proteins with conserved cysteines e.g. Rv1804c, Rv1810, Rv3354, etc O33192 No cell wall and cell processes O33192 O33192 MMAR_2495 MSMEG_4689 Mb1716 NC_000962.3 Mycobrowser_v4 CDS 1915949 1916701 . + 0 Rv1691 Rv1691 Function unknown Conserved hypothetical protein Rv1691, MTCI125.13, len: 250 aa. Conserved hypothetical protein, similar to Q9S210|SCI51.30C|AL109848 Hypothetical protein from Streptomyces coelicolor (210 aa), FASTA score: opt: 556, E(): 6.4e-27, (50.6% identity in 180 aa overlap). O33193 No conserved hypotheticals O33193 O33193 ML1355 MMAR_2496 MSMEG_3754 Mb1717 NC_000962.3 Mycobrowser_v4 CDS 1916698 1917759 . + 0 Rv1692 Rv1692 Function unknown; probably involved in cellular metabolism Probable phosphatase Rv1692, (MTCI125.14), len: 353 aa. Probable phosphatase, some similarity to others e.g. PNPP_SCHPO|Q00472 4-nitrophenylphosphatase (269 aa), FASTA scores: opt: 214, E(): 1.3e-10, (29.5% identity in 241 aa overlap); and to NAGD_ECOLI|P15302 nagd protein from Escherichia coli (250 aa), FASTA scores: opt: 314, E(): 9.8e-08, (28.2% identity in 245 aa overlap). Also similar to AL109848|SCI51.28 hypothetical protein from Streptomyces coelicolor (343 aa), FASTA scores: opt: 768, E(): 0, (44.8% identity in 315 aa overlap). O33194 No intermediary metabolism and respiration O33194 GO:0008152,GO:0016787 O33194 ML1356 MMAR_2497 MSMEG_3753 Mb1718 NC_000962.3 Mycobrowser_v4 CDS 1921542 1922723 . + 0 Rv1697 Rv1697 Function unknown Conserved hypothetical protein Rv1697, (MTCI125.19), len: 393 aa. Conserved hypothetical protein, highly similar to Q49895|MLC1351.11C|U00021 Hypothetical protein of Mycobacterium leprae from cosmid L247 (430 aa), FASTA scores: opt: 2345, E(): 0, (90.6% identity in 393 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O33198 No conserved hypotheticals O33198 O33198 GO:0009229,GO:0004788,GO:0005524 O33198 ML1361 MMAR_2502 MSMEG_3748 Mb1723 NC_000962.3 Mycobrowser_v4 CDS 1931497 1932654 . - 0 Rv1705c PPE22 Function unknown PPE family protein PPE22 Rv1705c, (MTCI125.27c), len: 385 aa. PPE22, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, similar to many e.g. YX23_MYCTU|Q10813 hypothetical 41.1 kDa protein cy274.2 3 (404 aa), fasta scores: opt: 819, E(): 0, (46.2% identity in 413 aa overlap). P9WI19 No PE/PPE Q79FL6 P9WI19 P9WI19 MMAR_2516 Mb1731c NC_000962.3 Mycobrowser_v4 CDS 1918746 1919669 . + 0 Rv1695 ppnK Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [catalytic activity: ATP + NAD+ = ADP + NADP+]. Inorganic polyphosphate/ATP-NAD kinase PpnK (poly(P)/ATP NAD kinase) Rv1695, (MTCI125.17), len: 307 aa. PpnK, inorganic polyphosphate/ATP-NAD kinase (see citation below), equivalent to Q49897|MLC1351.13C|Z95117|PPNK_MYCLE inorganic polyphosphate/ATP-NAD kinase from Mycobacterium leprae (311 aa) (87.9% identity in 305 aa overlap). Also similar to many e.g. P37768|PPNK_ECOLI probable inorganic polyphosphate/ATP-NAD kinase (292 aa), FASTA scores: opt: 384, E(): 1.7e-23, (33.5% identity in 233 aa overlap); etc. Belongs to the NAD kinase family. P9WHV7 No intermediary metabolism and respiration 1U0R,1U0T,1Y3H,1Y3I P0A5S6 P9WHV7 GO:0003951,GO:0005737,GO:0008152,GO:0046872,GO:0005524 2.7.1.23 P9WHV7 ML1359 MMAR_2500 MSMEG_3750 Mb1721 NC_000962.3 Mycobrowser_v4 CDS 1919683 1921446 . + 0 Rv1696 recN Involved in recombinational repair of damaged DNA. Probable DNA repair protein RecN (recombination protein N) Rv1696, (MTCI125.18), len: 587 aa. Probable recN, DNA repair protein (see citation below), similar to many e.g. RECN_ECOLI|P05824 dna repair protein recN (553 aa), FASTA scores: opt: 508, E(): 1.9e-33, (31.5% identity in 587 aa overlap). Equivalent to Z95117|MLCB1351_12 recN from Mycobacterium leprae (587 aa), FASTA scores: (76.1% identit y in 589 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). P9WHI7 No information pathways P0A5U6 P9WHI7 GO:0006310,GO:0006281,GO:0005524 P9WHI7 ML1360 MMAR_2501 Mb1722 NC_000962.3 Mycobrowser_v4 CDS 1922745 1923689 . + 0 Rv1698 mctB Outer membrane channel Outer membrane protein MctB Rv1698, (MTCI125.20), len: 314 aa. MctB, mycobacterial copper transport protein B essential for Cu resistance and maintenance of low intracellular Cu levels (See Wolschendorf et al., 2011). Outer membrane protein (See Siroy et al., 2008) with predicted N-terminal signal sequence. Probable coiled-coil from aa 31 to 67. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). P9WJ83 No cell wall and cell processes P64883 P9WJ83 GO:0006811,GO:0005886,GO:0046930,GO:0015288,GO:0009279 ML1362 MMAR_2503 Mb1724 NC_000962.3 Mycobrowser_v4 CDS 1923829 1925589 . + 0 Rv1699 pyrG Pyrimidine biosynthesis (last step) [catalytic activity: ATP + UTP + glutamine = ADP + orthophosphate + CTP (ammonia can replace glutamine).] Probable CTP synthase PyrG Rv1699, (MTCI125.21), len: 586 aa. Probable pyrG, CTP synthase highly similar to many e.g. PYRG_ECOLI|P08398 ctp synthase from Escherichia coli (544 aa), FASTA scores: opt: 1786, E():0, (51.8% identity in 548 aa overlap). Contains PS00442 Glutamine amidotransferases class-I active site. P9WHK7 No intermediary metabolism and respiration P0A5U2 P9WHK7 GO:0003883,GO:0006541,GO:0006221,GO:0005524 6.3.4.2 P9WHK7 ML1363 MMAR_2504 MSMEG_3746 Mb1725 NC_000962.3 Mycobrowser_v4 CDS 1925582 1926205 . + 0 Rv1700 Rv1700 NUDIX hydrolases catalyze the hydrolysis of a variety of nucleoside diphoshate derivatives NUDIX hydrolase Rv1700, (MTCI125.22), len: 207 aa. Nudix hydrolase, equivalent to Q49891|MLC1351.08C|Z95117 Hypothetical protein from Mycobacterium leprae (177 aa), FASTA scores: (66.7% identity in 171 aa overlap); also similar to Q9S225|SCI51.15C|AL109848 Hypothetical protein from Streptomyces coelicolor (211 aa), FASTA scores: opt: 508, E(): 1.2e-27, (43.1% identity in 197 aa overlap); similar to P54570|ADPP_BACSU ADP-ribose pyrophosphatase (185 aa), FASTA scores: opt: 313, E(): 1.1e-06, (42.7% identity in 124 aa overlap). Belongs to the family of Nudix hydrolases I6X235 No information pathways 1MK1,1MP2,1MQE,1MQW,1MR2 O33199 I6X235 GO:0016787 I6X235 ML1364 MMAR_2505 MSMEG_3745 Mb1726 NC_000962.3 Mycobrowser_v4 CDS 1926202 1927137 . + 0 Rv1701 Rv1701 Sequence integration/recombination. Probable integrase/recombinase Rv1701, (MTCI125.23), len: 311 aa. Probable integrase/recombinase, similar to many e.g. XERD_ECOLI|P21891 integrase/recombinase xerd (298 aa), FASTA scores: opt: 583, E(): 0, (41.8% identity in 311 aa overlap). Also similar to other Mycobacterium tuberculosis integrase/recombinase proteins RV2894c|MTCY274.25c (43.1% identity in 304 aa overlap); and Rv2646|MTCY441.16 phiRv2 integrase (31.1% identity in 161 aa overlap). Equivalent to Z95117|MLCB1351_7 from Mycobacterium leprae (316 aa) (85.4% identity in 316 aa overlap). P9WF33 No insertion seqs and phages P67636 P9WF33 GO:0015074,GO:0007049,GO:0051301,GO:0007059,GO:0005737,GO:0006313,GO:0009037,GO:0003677 P9WF33 ML1365 MMAR_2506 MSMEG_3744 Mb1727 NC_000962.3 Mycobrowser_v4 CDS 1932694 1933878 . - 0 Rv1706c PPE23 Function unknown PPE family protein PPE23 Rv1706c, (MTCI125.28c), len: 394 aa. PPE23, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, similar to many e.g. YX23_MYCTU|Q10813 hypothetical 41.1 kDa protein cy274.23 (404 aa), fasta scores: opt: 841, E(): 3.9e-31, (46.8% identity in 408 aa overlap). P9WI17 No PE/PPE Q7D842 P9WI17 P9WI17 MMAR_1095,MMAR_2517 Mb1732c NC_000962.3 Mycobrowser_v4 CDS 1934482 1934649 . - 0 Rv1706A Rv1706A Function unknown Conserved hypothetical protein Rv1706A, len: 55 aa. Conserved hypothetical protein, similar to part of several probable export proteins e.g. Rv0783c|Z80226_28 from Mycobacterium tuberculosis (540 aa), FASTA scores: opt: 125, E(): 0.011, (52.85% identity in 53 aa overlap). Size difference suggests possible gene fragment. Q79FL4 No conserved hypotheticals Q79FL4 Mb1733c NC_000962.3 Mycobrowser_v4 CDS 1936360 1937316 . + 0 Rv1708 Rv1708 Unknown; thought to be involved in cell process. Putative initiation inhibitor protein Rv1708, (MTCI125.30), len: 318 aa. Putative initiation inhibitor protein, a soj-related protein probably involved in cell process, highly similar to many sporulation initiation inhibitor proteins soj e.g. P37522|SOJ_BACSU Soj protein from Bacillus subtilis (253 aa), FASTA scores: opt: 745, E(): 0, (46.0% identity in 248 aa overlap), and more weakly to various repA/para/incC proteins from various organisms e.g. Y4CK_RHISN|P55393 putative replication protein A from Rhizobium sp. (407 aa), FASTA scores: opt: 205, E(): 4e-13, (29.0% identity in 252 aa overlap). Also similar to Mycobacterium tuberculosis hyothetical proteins Rv3213c and Rv3918c. P9WLT1 No cell wall and cell processes O33207 P9WLT1 GO:0005524 P9WLT1 ML1367 MMAR_2523 MSMEG_3743 Mb1735 NC_000962.3 Mycobrowser_v4 CDS 1944406 1944756 . + 0 Rv1717 Rv1717 Function unknown Conserved hypothetical protein Rv1717, (MTCY04C12.02), len: 116 aa. Conserved hypothetical protein, similar to O29060|AF1208|AE001021 Hypothetical protein from Arecheoglobus fulgidus (114 aa), FASTA scores: opt: 254, E(): 3.3e-09, (37.7% identity in 114 aa overlap). O86372 No conserved hypotheticals O86372 O86372 O86372 Mb1745 NC_000962.3 Mycobrowser_v4 CDS 1989042 1989566 . + 0 Rv1758 cut1 Hydrolysis of cutin. Probable cutinase Cut1 Rv1758, (MTCY28.24), len: 174 aa. Probable cut1, serine esterase, cutinase family, similar to Rv2301|CUT2_MYCTU|Q50664 probable cutinase cy339.08c precursor from Mycobacterium tuberculosis (219 aa), FASTA scores: opt: 369, E(): 1. 1e-16, (39.1% identity in 179 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical cutinases Rv3452, Rv1984c, Rv3451 and Rv3724. CDS has been interrupted by IS6110 insertion element and 5'-end deleted. Belongs to the cutinase family. O06793 No cell wall and cell processes O06793 O06793 GO:0008152,GO:0016787 3.1.1.- O06793 MSMEG_2474,MSMEG_4465,MSMEG_6354 Mb1788 NC_000962.3 Mycobrowser_v4 CDS 2282099 2282740 . + 0 Rv2036 Rv2036 Unknown Conserved hypothetical protein Rv2036, (MTV018.23), len: 213 aa. Conserved hypothetical protein; similar to many. O53480 No conserved hypotheticals O53480 O53480 O53480 Mb2062 NC_000962.3 Mycobrowser_v4 CDS 1938146 1938841 . + 0 Rv1710 scpB Involved in chromosome structure and partitioning Possible segregation and condensation protein ScpB Rv1710, (MTCI125.32), len: 231 aa. Possible scpB, segregation and condensation protein, similar to several hypothetical proteins e.g. P35155|YPUH_BACSU from Bacillus subtilis (197 aa), FASTA scores: opt: 339, E(): 1.3e-09, (36.0% identity in 186 aa overlap); Q9S231|SCI51.09C|AL109848 from Streptomyces coelicolor (223 aa), FASTA scores: opt: 626, E(): 0, (51.0% identity in 192 aa overlap). Equivalent to O05669|MLC1351.04C|Z95117 Hypothetical protein from Mycobacterium leprae (231 aa), FASTA scores: (77.9% identity in 231 aa overlap). I6XCB2 No cell wall and cell processes 2Z99 O33209 I6XCB2 GO:0005737,GO:0007049,GO:0051301,GO:0007059 I6XCB2 ML1369 MMAR_2525 MSMEG_3741 Mb1737 NC_000962.3 Mycobrowser_v4 CDS 1938838 1939602 . + 0 Rv1711 Rv1711 Function unknown Conserved hypothetical protein Rv1711, (MTCI125.33), len: 254 aa. Conserved hypothetical protein, highly similar to a large family of hypothetical proteins e.g. P37765|YCIL_ECOLI from Escherichia coli (291 aa), FASTA scores: opt: 496, E(): 1.1e-29, (41.6% identity in 250 aa overlap); 9S232|SCI51.08C|AL109848 putative pseudouridine synthase from Streptomyces coelicolor (371 aa), FASTA scores: opt: 818, E(): 0, (53.1% identity in 245 aa overlap). Equivalent to O05668|MLCB1351.03C|Z95117 Hypothetical protein from Mycobacterium leprae (256 aa), (80.5% identity in 256 aa overlap). Contains PS01149 Hypothetical yciL/yejD/yjbC family signature. P9WHQ1 No conserved hypotheticals P65842 P9WHQ1 GO:0009982,GO:0001522,GO:0003723 5.4.99.- P9WHQ1 ML1370 MMAR_2526 MSMEG_3740 Mb1738 NC_000962.3 Mycobrowser_v4 CDS 1939599 1940291 . + 0 Rv1712 cmk Catalyzes the transfer of a phosphate group from ATP to either CMP or dCMP to form CDP or dCDP and ADP [catalytic activity: ATP + CMP = ADP + CDP]. Cytidylate kinase Cmk (CMP kinase) (cytidine monophosphate kinase) (ck) Rv1712, (MTCI125.34), len: 230 aa. cmk, cytidylate kinase, highly similar to many e.g. KCY_ECOLI|P23863 cytidylate kinase from Escherichia coli (227 aa), FASTA scores: opt: 534, E (): 0, (40.3% identity in 221 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Equivalent to Z95117|MLCB1351_2 from Mycobacterium leprae (223 aa) (73.5% identity in 226 aa overlap). Belongs to the cytidylate kinase family, subfamily 1. P9WPA9 No intermediary metabolism and respiration P63803 P9WPA9 GO:0004127,GO:0005737,GO:0006220,GO:0005524 2.7.4.14 P9WPA9 ML1371 MMAR_2527 MSMEG_3739 Mb1739 NC_000962.3 Mycobrowser_v4 CDS 1940288 1941679 . + 0 Rv1713 engA Binds both GDP and GTP. Has an intrinsic GTPase activity and is essential for cell growth. Probable GTP-binding protein EngA Rv1713, (MTCI125.35), len: 463 aa. Probable engA, GTP-binding protein. Equivalent to Q49884|MLCB1351.01|U00021_5 probable GTP-binding protein ENGA from Mycobacterium leprae (461 aa), (88.6% identity in 463 aa overlap). And similar to many e.g. P50743|ENGA_BACSU probable GTP-binding protein ENGA from Bacillus subtilus (436 aa), FASTA scores: opt: 1077, E(): 0, (40.6% identity in 434 aa overlap). Contains two PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the era/TRME family of GTP-binding proteins. ENGA subfamily. P9WNL3 No intermediary metabolism and respiration P64057 P9WNL3 GO:0005622,GO:0017111,GO:0005525 P9WNL3 ML1372 MMAR_2528 MSMEG_3738 Mb1740 NC_000962.3 Mycobrowser_v4 CDS 1941853 1942665 . + 0 Rv1714 Rv1714 Function unknown; probably involved in cellular metabolism Probable oxidoreductase Rv1714, (MTV048.01), len: 270 aa. Probable oxidoreductase similar to many e.g. AE0010|AE001021_4 Archaeoglobus fulgidus section 79 (281 aa), FASTA scores: opt: 578, E(): 3.3e-31, (38.9% identity in 265 aa overlap). Also similar to several other M. tuberculosis oxidoreductases e.g. Rv1544, etc. P9WGQ3 No intermediary metabolism and respiration O53927 P9WGQ3 GO:0055114,GO:0016491 1.-.-.- P9WGQ3 Mb1741 NC_000962.3 Mycobrowser_v4 CDS 1953864 1954634 . - 0 Rv1728c Rv1728c Function unknown Conserved hypothetical protein Rv1728c, (MTCY04C12.13c), len: 256 aa. Conserved hypothetical protein, some similarity to O07246|Rv0320|MTCY63.25 possible exported protein from Mycobacterium tuberculosis (220 aa), FASTA scores: E(): 1.3e-31, (42.3% identity in 220 aa overlap). C-terminal region similar to Q9ZX60|AF068845|AF068845_17 segment of gp17 of Mycobacteriophage TM4 (1229 aa), FASTA scores: opt: 385, E(): 4.3e-17, (44.6% identity in 139 aa overlap). P71986 No conserved hypotheticals P71986 MSMEG_3714 Mb1757c NC_000962.3 Mycobrowser_v4 CDS 1960774 1961016 . - 0 Rv1734c Rv1734c Function unknown Conserved hypothetical protein Rv1734c, (MTCY04C12.19c), len: 80 aa. Conserved hypothetical protein, similar to C-terminal region Q9Z8N2|CP0452|AE001615 Dihydrolipoamide Acetyltransferase from Chlamydia pneumoniae (429 aa), FASTA scores: opt: 138, E(): 0.0012, (26.9% identity in 78 aa overlap). P9WLS7 No conserved hypotheticals P71992 P9WLS7 GO:0008152,GO:0008415 Mb1763c NC_000962.3 Mycobrowser_v4 ncRNA 1952291 1952503 . - 0 MTB000055 AS1726 Unknown Putative small regulatory RNA AS1726, putative small regulatory RNA (See Arnvig and Young, 2009). Alternate 5'-ends at positions 1952400, 1952375, 1952367, and 1952351. No stable RNAs NC_000962.3 Mycobrowser_v4 CDS 1943576 1944406 . + 0 Rv1716 Rv1716 Function unknown Conserved hypothetical protein Rv1716, (MTV048.03,MTCY04C12.01), len: 276 aa. Conserved hypothetical protein, shows high similarity with AF1200|O29068|AE001021_11A conserved protein of Archaeoglobus fulgidus, gp fulgidus section 7 (278 aa), FASTA scores: E(): 0, (61.8% identity in 251 a a overlap); also weak similarity to several polyketide cyclases e.g. O68500|AF048833|DPSY from Streptomyces peucetius (272 aa), FASTA scores: opt: 194, E(): 1.7e-05, (29.6% identity in 223 aa overlap). O53929 No conserved hypotheticals O53929 O53929 O53929 ML1374 Mb1744 NC_000962.3 Mycobrowser_v4 CDS 1945641 1946420 . + 0 Rv1719 Rv1719 Involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv1719, (MTCY04C12.04), len: 259 aa. Probable transcriptional regulatory protein, similar to YIAJ_ECOLI|P37671 hypothetical transcriptional regulator from Escherichia coli (282 aa), FASTA scores: opt: 353, E(): 3.2e-15, (31.1% identity in 235 aa overlap). Similar to Mycobacterium tuberculosis hypothetical IclR-family transcriptional regulators Rv2989, Rv1773c. Helix-turn-helix motif from aa 34-55 (+6.94 SD). P71977 No regulatory proteins P71977 P71977 GO:0006355,GO:0006350,GO:0003700,GO:0005622 P71977 ML1375 Mb1748 NC_000962.3 Mycobrowser_v4 CDS 1947030 1947419 . - 0 Rv1720c vapC12 Unknown Possible toxin VapC12 Rv1720c, (MTCY04C12.05c), len: 129 aa. Possible vapC12, toxin, part of toxin-antitoxin (TA) operon with Rv1721c, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to other Mycobacterium tuberculosis hypothetical proteins e.g. O53610|Rv0065|MTV030.08 (133 aa), FASTA scores: E(): 1.5e-10, (39.1% identity in 128 aa overlap); P71550|Rv0960|MTCY10D7.14C (129 aa) and O06415|Rv0549c|MTCY25D10.28C (137 aa). P9WFA3 No virulence, detoxification, adaptation P9WFA3 P9WFA3 Mb1749c NC_000962.3 Mycobrowser_v4 CDS 1949342 1950589 . + 0 Rv1723 Rv1723 Function unknown; probably involved in cellular metabolism Probable hydrolase Rv1723, (MTCY04C12.08), len: 415 aa. Possible hydrolase, similar to others e.g. NYLB_FLASP|P07061 6-aminohexanoate-dimer hydrolase from Flavobacterium sp. (392 aa), FASTA scores: opt: 717, E(): 0, (35.1% identity in 396 aa overlap). Also similar to M. tuberculosis hypothetical esterases and penicillin binding proteins e.g. Rv1923, Rv1497, Rv2463, etc P71981 No intermediary metabolism and respiration P71981 P71981 GO:0016787 3.-.-.- P71981 MMAR_3387 Mb1752 NC_000962.3 Mycobrowser_v4 CDS 1951852 1953237 . + 0 Rv1726 Rv1726 Function unknown; probably involved in cellular metabolism Probable oxidoreductase Rv1726, (MTCY04C12.11), len: 461 aa. Probable oxidoreductase, similar to HDNO_ARTOX|P08159 6-hydroxy-d-nicotine oxidase (458 aa), FASTA scores: opt: 678, E(): 0, (29.5% identity in 465 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical dehydrogenases e.g. Rv3107c, Rv1257c, etc. P71984 No intermediary metabolism and respiration P71984 P71984 GO:0055114,GO:0016491,GO:0050660 1.-.-.- P71984 MSMEG_1746 Mb1755 NC_000962.3 Mycobrowser_v4 CDS 1953270 1953839 . + 0 Rv1727 Rv1727 Function unknown Conserved hypothetical protein Rv1727, (MTCY04C12.12), len: 189 aa. Conserved hypothetical protein, similar to Mycobacterium tuberculosis hypothetical proteins P72040|Rv3773c|MTCY13D12.07C (194 aa), FASTA scores: opt: 176, E(): 2.7e-08, (31.1% identity in 180 aa overlap); and O53801|Rv0738 (182 aa). P71985 No conserved hypotheticals P71985 P71985 Mb1756 NC_000962.3 Mycobrowser_v4 CDS 1955692 1957245 . - 0 Rv1730c Rv1730c Thought to be involved in cell wall biosynthesis and may also act as a sensor of external penicillins Possible penicillin-binding protein Rv1730c, (MTCY04C12.15c), len: 517 aa. Possible penicillin-binding protein, similar to others e.g. PBP4_NOCLA|Q06317 penicillin-binding protein 4 (pbp-4) from Nocardia lactamdurans (381 aa), FASTA scores: opt: 643, E(): 3.8e-32, (33.8% identity in 370 aa overlap); etc. Also similar to other Mycobacterium tuberculosis hypothetical penicillin binding proteins and esterases e.g. Rv1923, Rv1497, etc. P71988 No cell wall and cell processes P71988 P71988 P71988 Mb1759c NC_000962.3 Mycobrowser_v4 CDS 1959243 1959791 . - 0 Rv1732c Rv1732c Function unknown Conserved protein Rv1732c, (MTCY04C12.17c), len: 182 aa. Conserved protein, highly similar to hypothetical proteins from several organisms e.g. P73178|SLL1289|D90904 from Synechocystis (194 aa), FASTA scores: opt: 663, E(): 0, (53.1% identity in 179 aa overlap); etc. P71990 No conserved hypotheticals P71990 P71990 GO:0045454,GO:0016491,GO:0016209 P71990 ML1418,ML1418c MMAR_2937 Mb1761c NC_000962.3 Mycobrowser_v4 CDS 1959855 1960487 . - 0 Rv1733c Rv1733c Unknown Probable conserved transmembrane protein Rv1733c, (MTCY04C12.18c), len: 210 aa. Probable conserved transmembrane protein. Similar to AL109962|SCJ1_26 hypothetical protein from Streptomyces coelicolor (193 aa), FASTA scores: opt: 287, E(): 3.8e-11, (35.2% identity in 182 aa overlap). Predicted possible vaccine candidate (See Zvi et al., 2008). P9WLS9 No cell wall and cell processes P9WLS9 GO:0005886,GO:0016021 MMAR_3501 MSMEG_1127,MSMEG_1738,MSMEG_6676 Mb1762c NC_000962.3 Mycobrowser_v4 CDS 2026477 2026776 . + 0 Rv1788 PE18 Function unknown PE family protein PE18 Rv1788, (MTV049.10), len: 99 aa. PE18, Member of the Mycobacterium tuberculosis PE family of gly-, ala-rich proteins (see citation below), similar to Z93777|MTCI364.07 Mycobacterium tuberculosis cosmid (99 aa), FASTA scores: opt: 414, E(): 3.6e-20, (72.4% identity in 98 aa overlap). L7N649 No PE/PPE Q7D7Y9 L7N649 L7N649 MMAR_2670 Mb1816 NC_000962.3 Mycobrowser_v4 CDS 1965657 1965941 . + 0 Rv1738 Rv1738 Function unknown Conserved protein Rv1738, (MTCY04C12.23), len: 94 aa. Conserved protein, similar to P71931|Rv2632c|YQ32_MYCTU Hypothetical 10.1 kDa protein from Mycobacterium tuberculosis (93 aa), FASTA scores: opt: 319, E(): 2.6e-27, (53.9% identity in 89 aa overlap). Predicted possible vaccine candidate (See Zvi et al., 2008). P9WLS3 No conserved hypotheticals P64887 P9WLS3 P9WLS3 Mb1767 NC_000962.3 Mycobrowser_v4 CDS 1967917 1968165 . + 0 Rv1741 vapC34 Unknown Possible toxin VapC34. Contains PIN domain. Rv1741, (MTCY28.03,MTCY04C12.26), len: 82 aa. Possible vapC34, toxin, part of toxin-antitoxin (TA) operon with Rv1740, contains PIN domain, see Arcus et al. 2005. Similar in N-terminus to others in Mycobacterium tuberculosis e.g. P96914|Rv0624|MTCY20H10.05 (131 aa), (80.4% identity in 56 aa overlap); P71999|Rv1741 (82 aa) and O07769|Rv0609 (133 aa). P9WF71 No virulence, detoxification, adaptation P9WF71 P9WF71 Mb1770 NC_000962.3 Mycobrowser_v4 CDS 1968173 1968910 . + 0 Rv1742 Rv1742 Unknown Unknown protein Rv1742, (MTCY28.04,MTCY04C12.27), len: 245 aa. Unknown protein. O33271 No unknown O33271 MMAR_2580 MSMEG_6830 Mb1771 NC_000962.3 Mycobrowser_v4 CDS 1986854 1987696 . - 0 Rv1755c plcD Hydrolyzes sphingomyelin in addition to phosphatidylcholine. Probable virulence factor implicated in the pathogenesis of M.tuberculosis at the level of intracellular survival, by the alteration of cell signaling events or by direct cytotoxicity [catalytic activity: a phosphatidylcholine + H(2)O = 1,2- diacylglycerol + choline phosphate]. Probable phospholipase C 4 (fragment) PlcD Rv1755c, (MT1799, MTCY28.21c), len: 280 aa. Probable plcD, phospholipase C 4 (fragment) (see citations below), highly similar to C-terminus of other phospholipases e.g. CQ50771|Rv2351c|PLCA|MTP40|MT2416|MTCY98.20c phospholipase C 1 from Mycobacterium tuberculosis (512 aa), FASTA score: (71.1% identity in 284 aa overlap); etc. Note that this ORF has been interrupted by insertion of IS6110 element. Belongs to the bacterial phospholipase C family. P9WIA9 No intermediary metabolism and respiration P0A5R8 P9WIA9 GO:0034480 3.1.4.3 P9WIA9 NC_000962.3 Mycobrowser_v4 CDS 1987745 1988731 . - 0 Rv1756c Rv1756c Required for the transposition of the insertion element IS6110. Putative transposase Rv1756c, (MTCY28.22c), len: 328 aa. Putative Transposase subunit for IS6110. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv1756c and Rv1757c, the sequence UUUUAAAG (directly upstream of Rv1756c) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 34 aa) P9WKH9 No insertion seqs and phages P0C5G8 P9WKH9 GO:0015074,GO:0006310,GO:0032196,GO:0003677 P9WKH9 NC_000962.3 Mycobrowser_v4 CDS 2000614 2002470 . + 0 Rv1768 PE_PGRS31 Function unknown PE-PGRS family protein PE_PGRS31 Rv1768, (MTCY28.34), len: 618 aa. PE_PGRS31, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), highly similar to Q50615 hypothetical 40.8 kDa protein (498 aa), FASTA scores: opt: 1703, E(): 0, (57.4% identity in 566 aa overlap). Q79FK9 No PE/PPE Q79FK9 Q79FK9 Q79FK9 MMAR_2645 Mb1797 NC_000962.3 Mycobrowser_v4 CDS 1970989 1971390 . - 0 Rv1744c Rv1744c Unknown Probable membrane protein Rv1744c, (MTCY28.06c), len: 133 aa. Probable membrane protein, contains four imperfect 10 aa repeats, some similarity to Q25946 (MSA-2) (fragment) from Plasmodium falciparum (205 aa), FASTA scores: opt: 145, E( ): 0.048, (52.4% identity in 63 aa overlap). O06787 No cell wall and cell processes O06787 O06787 Mb1773c NC_000962.3 Mycobrowser_v4 CDS 1971380 1971991 . - 0 Rv1745c idi Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopenten to its allylic isomer, dimethylallyl diphosphate (DMAPP) [catalytic activity :isopentenyl diphosphate = dimethylallyl diphosphate] Probable isopentenyl-diphosphate delta-isomerase Idi (IPP isomerase) (isopentenyl pyrophosphate isomerase) Rv1745c, (MTCY28.08c,MTCY04C12.29c), len: 203 aa. Probable idi, isopentenyl-diphosphate delta-isomerase, similar to Q46822|ORF_O182 from Escherichia coli (182 aa), FASTA scores: opt: 465, E(): 4.7e-25, (46.9% identity in 162 aa overlap), and to IPPI_SCHPO|Q10132 isopentenyl-diphosphate delta-isomerase from Schizosaccharomyces pombe (227 aa), FASTA scores: opt: 185, E(): 5.4e-06, (30.3% identity in 152 aa overlap). Belongs to the IPP isomerase type 1 family. P9WKK5 No intermediary metabolism and respiration P72002 P9WKK5 GO:0016787,GO:0004452,GO:0008299,GO:0046872,GO:0005737 5.3.3.2 P9WKK5 MMAR_3218 Mb1774c NC_000962.3 Mycobrowser_v4 CDS 1972138 1973568 . + 0 Rv1746 pknF Involved in signal transduction (via phosphorylation). Thought to be involved in membrane transport. Phosphorylates the peptide substrate myelin basic protein (MBP) at serine and threonine residues [catalytic activity: ATP + a protein = ADP + a phosphoprotein]. Anchored-membrane serine/threonine-protein kinase PknF (protein kinase F) (STPK F) Rv1746, (MTCY28.09, MTCY04C12.30), len: 476 aa. pknF, transmembrane serine/threonine-protein kinase (see citations below), highly similar to KY28_MYCTU|Q10697 probable serine/threonine-protein kinase from Mycobacterium tuberculosis (589 aa), FASTA scores: opt: 870, E(): 0, (41.6% identity in 406 aa overlap). Contains PS00108 Serine/Threonine protein kinases active-site signature. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases. Experimental studies show evidence of auto-phosphorylation. Start site chosen by homology, may extend further upstream. P9WI75 No regulatory proteins P72003 P9WI75 GO:0006468,GO:0004674,GO:0005524 2.7.11.1 P9WI75 ML1386 MMAR_2606 MSMEG_3677 Mb1775 NC_000962.3 Mycobrowser_v4 CDS 1973630 1976227 . + 0 Rv1747 Rv1747 Thought to be involved in active transport of undetermined substrate (possibly lipooligosaccharide) across the membrane. Responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane. Probable conserved transmembrane ATP-binding protein ABC transporter Rv1747, (MTCY28.10, MTCY04C12.31), len: 865 aa. Probable conserved transmembrane ATP-binding protein ABC transporter (see citation below), similar to others e.g Q55956 ABC transporter from Synechocystis sp. (790 aa), FASTA scores: opt: 738, E(): 6.3e-26, (31.6% identity in 632 aa overlap); etc. Also similar to other M. tuberculosis ABC-type transporters e.g. Rv2397c|MTCY253.24, FASTA score: (35.2% identity in 213 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). O65934 No cell wall and cell processes O65934 O65934 GO:0016887,GO:0016021,GO:0005524 O65934 ML1385,ML1385c MMAR_2612 MSMEG_1642 Mb1776 NC_000962.3 Mycobrowser_v4 CDS 1976600 1977331 . + 0 Rv1748 Rv1748 Unknown Unknown protein Rv1748, (MTCY28.11, MTCY04C12.32), len: 243 aa. Unknown protein. Possibly exported protein, hydrophobic domain, TM helix aa 23-45. P72005 No conserved hypotheticals P72005 MMAR_2624 Mb1777 NC_000962.3 Mycobrowser_v4 CDS 1979621 1981003 . + 0 Rv1751 Rv1751 Function unknown; probably involved in cellular metabolism Probable oxidoreductase Rv1751, (MTCY28.14-MTCY04C12.35), len: 460 aa. Probable oxidoreductase, possibly a monooxygenase or hydroxylase, similar to MHPA_ECOLI|P77397 3-(3-hydroxy-phenyl) propionate hydroxylase (554 aa), FASTA scores: opt: 239, E(): 2e-08, (24.6% identity in 435 aa overlap); and AJ007932|SAR7932.13 oxygenase from Streptomyces argillaceus (436 aa), FASTA scores: opt: 587, E(): 8.6e-30, (32.3% identity in 359 aa overlap). Contains PS00075 Dihydrofolate reductase signature. Also similar to Mycobacterium tuberculosis hypothetical oxidoreductases Rv1260 and Rv0575c. O65936 No intermediary metabolism and respiration O65936 O65936 GO:0055114,GO:0004497 1.-.-.- O65936 MMAR_2627 MSMEG_4962 Mb1780 NC_000962.3 Mycobrowser_v4 CDS 1981614 1984775 . - 0 Rv1753c PPE24 Function unknown PPE family protein PPE24 Rv1753c, (MTCY28.16c), len: 1053 aa. PPE24, Member of the Mycobacterium tuberculosis PPE family of Gly-, Asn-rich proteins, similar to many e.g. YF48_MYCTU|Q10778 hypothetical protein cy48.17 (678 aa), FASTA scores: opt: 1360, E(): 0, (48.9% identity in 550 aa overlap). Note that the Gly-, Asn-rich sequence is interrupted by six near-perfect 26 aa repeats, a unique region, and another, more degenerate region of five 25 aa repeats before resuming at the C-terminus. The end of the first Gly-, Asn- rich region and the start of the first set of repeats shows some similarity to Q50577|AT10S from Mycobacterium tuberculosis (170 aa) (40.2% identity in 189 aa overlap). P9WI15 No PE/PPE Q79FL2 P9WI15 P9WI15 MMAR_0932,MMAR_1028,MMAR_2822 Mb1782c NC_000962.3 Mycobrowser_v4 CDS 1984979 1986670 . - 0 Rv1754c Rv1754c Function unknown Conserved protein Rv1754c, (MTCY28.17c), len: 563 aa. Conserved protein, has proline-rich central region. Some similarity in central region to other Mycobacterium tuberculosis proline-rich proteins e.g. O06555|Rv1157c|MTCI65.24c (371 aa), (32.5% identity in 191 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06790 No conserved hypotheticals O06790 O06790 ML2491 MMAR_2586 MSMEG_2107 Mb1783c NC_000962.3 Mycobrowser_v4 CDS 2003878 2005164 . + 0 Rv1770 Rv1770 Function unknown Conserved protein Rv1770, (MTCY28.36), len: 428 aa. Conserved protein, highly similar in N-terminus to Q49882 Hypothetical protein from Mycobacterium leprae from cosmid L247 (83 aa), FASTA scores: opt: 301, E(): 1e-12, (56.5% identity in 85 aa overlap). O06803 No conserved hypotheticals O06803 O06803 GO:0006508,GO:0008233 ML1378 MMAR_2647 Mb1799 NC_000962.3 Mycobrowser_v4 CDS 1993153 1994661 . + 0 Rv1760 Rv1760 May be involved in synthesis of triacylglycerol Possible triacylglycerol synthase (diacylglycerol acyltransferase) Rv1760, (MTCY28.26), len: 502 aa. Possible triacylglycerol synthase (See Daniel et al., 2004), similar to several other Mycobacterium tuberculosis proteins e.g. Q10554|Y895_MYCTU|MTCY31.23 (505 aa), FASTA scores: opt: 692, E(): 0, (31.7% identity in 477 aa overlap). Member of family with at least 15 other members e.g. Rv3740c, Rv3734c, Rv1425, etc. P9WKB9 No lipid metabolism O06795 P9WKB9 GO:0006071,GO:0008610,GO:0004144 2.3.1.20 MMAR_2634 Mb1791 NC_000962.3 Mycobrowser_v4 CDS 1996152 1996478 . + 0 Rv1763 Rv1763 Required for the transposition of the insertion element IS6110. Putative transposase for insertion sequence element IS6110 (fragment) Rv1763, (MTCY28.29), len: 108 aa. Putative Transposase for IS6110 (fragment), identical to many other Mycobacterium tuberculosis IS6110 transposase subunits e.g. Q50686|YIA4_MYCTU Insertion element IS6110 hypothetical 12.0 kDa protein (108 aa), fasta scores: E(): 1.4e-43, (100.00% identity in 108 aa overlap). The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv1763 and Rv1764, the sequence UUUUAAAG (directly upstream of Rv1764) maybe responsible for such a frameshifting event (see McAdam et al., 1990). P9WKH5 No insertion seqs and phages P0C5G9 P9WKH5 GO:0004803,GO:0006313,GO:0003677 P9WKH5 NC_000962.3 Mycobrowser_v4 CDS 1997418 1998515 . - 0 Rv1765c Rv1765c Function unknown Conserved hypothetical protein Rv1765c, (MTCY28.31c), len: 365 aa. Conserved hypothetical protein, highly similar to O53461|Rv2015c|MTV018.02c conserved hypothetical protein (418 aa), (97.8% identity in 364 aa overlap). Blast hits with non-is part of sequence submitted under MTU78639. O06798 No conserved hypotheticals O06798 O06798 GO:0003676,GO:0004519 MSMEG_3404 Mb1794c NC_000962.3 Mycobrowser_v4 CDS 1999142 1999357 . - 0 Rv1765A Rv1765A Possibly required for the transposition of an insertion element. Putative transposase (fragment) Rv1765A, len: 71 aa. Putative transposase (fragment), similar to part of many transposase genes including IS6110 e.g. P19774|TRA9_MYCTU putative transposase from Mycobacterium tuberculosis (278 aa), FASTA scores: opt: 231, E(): 4.7e-11, (45.35% identity in 75 aa overlap). Q79FL0 No insertion seqs and phages Q79FL0 Q79FL0 GO:0015074,GO:0003677 Mb1795c NC_000962.3 Mycobrowser_v4 CDS 1999737 2000006 . + 0 Rv1766 Rv1766 Function unknown Conserved protein Rv1766, (MTCY28.32), len: 89 aa. Conserved protein, highly similar to P54431|YRKD_BACSU Hypothetical 7.0 kDa protein in bltr-spoIIIC intergenic region from Bacillus subtilis (63 aa), FASTA scores: opt: 151, E(): 1.5e-05, (53.3% identity in 45 aa overlap). Also similar to Q9RD62|SCF56.04C|AL133424 Hypothetical protein from Streptomyces coelicolor (92 aa), FASTA scores: opt: 239, E(): 1.3e-11, (62.5% identity in 64 aa overlap). Also some similarity to other Mycobacterium tuberculosis hypothetical proteins e.g. O07434|Rv0190|MTCI28.29 (96 aa), (35.5% identity in 62 aa overlap); P71543|Rv0967 (119 aa), and P71600|Rv0030 (109 aa). Start changed since original submission. O06799 No conserved hypotheticals O06799 O06799 O06799 MMAR_2642 MSMEG_5388 NC_000962.3 Mycobrowser_v4 CDS 2881758 2882147 . + 0 Rv2562 Rv2562 Function unknown Conserved hypothetical protein Rv2562, (MTCY9C4.06c), len: 129 aa. Conserved hypothetical protein, highly similar, but shorter 83 aa, to downstream ORF AAK46951|RV2561|MT2638|MTCY9C4.07c conserved hypothetical protein from Mycobacterium tuberculosis (97 aa), FASTA scores: opt: 866, E(): 2.2e-54, (100.0% identity in 129 aa overlap). Q50737 No conserved hypotheticals Q50737 Q50737 P9WL99 Mb2591 NC_000962.3 Mycobrowser_v4 CDS 2007020 2007766 . - 0 Rv1773c Rv1773c Involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv1773c, (MTCY28.39), len: 248 aa. Probable transcriptional regulator belonging to IclR family, similar to ICLR_ECOLI|P16528 acetate operon repressor from Escherichia coli (274 aa), FASTA scores: opt: 261, E(): 3.3e-10, (26.9% identity in 249 aa overlap). Also similar to Mycobacterium tuberculosis protein Rv1719|MTCY04C12.04 (40.2% identity in 244 aa overlap); and Rv2989. Start site chosen by homology, but may extend further upstream. Contains possible helix-turn-helix motif at aa 37-58 (+3.24 SD). O06806 No regulatory proteins O06806 O06806 GO:0006355,GO:0006350,GO:0003677 O06806 MSMEG_2483,MSMEG_6757 Mb1802c NC_000962.3 Mycobrowser_v4 CDS 2007832 2009172 . + 0 Rv1774 Rv1774 Function unknown; probably involved in cellular metabolism Probable oxidoreductase Rv1774, (MTCY25C11.01), len: 446 aa. Probable oxidoreductase, similar to several e.g. HDNO_ARTOX|P08159 6-hydroxy-d-nicotine oxidase (458 aa), FASTA scores: opt: 417, E(): 6e-20, (28.4% identity in 462 aa overlap). Also some similarity to Mycobacterium tuberculosis oxidoreductase MTCY04C12.11 (24.1% identity in 444 aa overlap). Contains PS00862 Oxygen oxidoreductases covalent FAD-binding site. O33177 No intermediary metabolism and respiration O33177 O33177 GO:0055114,GO:0016491,GO:0050660 1.-.-.- O33177 Mb1803 NC_000962.3 Mycobrowser_v4 CDS 2009995 2010555 . - 0 Rv1776c Rv1776c Involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv1776c, (MTCY25C11.03c), len: 186 aa. Possible regulatory protein, some similarity to Mycobacterium tuberculosis Rv1255c|Q11063 hypothetical transcriptional regulator (202 aa), FASTA scores: opt: 270, E(): 9.7e-09, (28.3% identity in 191 aa overlap). Contains possible helix-turn-helix motif at aa 37-58 (+3.49 SD). O33179 No regulatory proteins O33179 O33179 GO:0006350,GO:0003700,GO:0006355 O33179 MMAR_2651 MSMEG_0676 Mb1805c NC_000962.3 Mycobrowser_v4 CDS 2012686 2014479 . - 0 Rv1779c Rv1779c Unknown Possible integral membrane protein Rv1779c, (MTV049.01c), len: 597 aa. Possible integral membrane protein. O53930 No cell wall and cell processes O53930 O53930 MMAR_2660 Mb1808c NC_000962.3 Mycobrowser_v4 CDS 2014699 2015262 . + 0 Rv1780 Rv1780 Function unknown Conserved protein Rv1780, (MTV049.02), len: 187 aa. Conserved protein, equivalent to Q49881|ML1380|U00021_2 cosmid L247 from Mycobacterium leprae (187 aa), FASTA scores: opt: 1000, E(): 0, (82.4% identity in 187 aa overlap). O53931 No conserved hypotheticals O53931 ML1380 MMAR_2661 MSMEG_3683 Mb1809 NC_000962.3 Mycobrowser_v4 CDS 2048398 2049597 . + 0 Rv1807 PPE31 Function unknown PPE family protein PPE31 Rv1807, (MTV049.29), len: 399 aa. PPE31, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, most similar to Rv1789|MTV049.11|AL022021 (393 aa), FASTA scores: opt: 1169, E(): 0, (49.5% identity in 412 aa overlap). L0T7Y7 No PE/PPE Q79FJ7 L0T7Y7 L0T7Y7 ML1533,ML1533c MMAR_2683 Mb1836 NC_000962.3 Mycobrowser_v4 CDS 2024828 2025031 . + 0 Rv1786 Rv1786 Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Probable ferredoxin Rv1786, (MTV049.08), len: 67 aa. Probable ferredoxin, similar to others e.g. X63601|FERS_STRGR ferredoxin from Streptomyces griseus (65 aa), FASTA scores: opt: 140, E(): 0.001, (38.1% identity in 63 aa overlap); T50943 probable ferredoxin DitA from Pseudomonas abietaniphila (78 aa); BAA84714.1|AB017795 ferredoxin from Nocardioides sp. (69 aa); etc. Also similar to Rv0763c|MTCY369.08 from Mycobacterium tuberculosis (68 aa), FASTA score: (30.6% identity in 62 aa overlap); and Rv0763c. O53937 No intermediary metabolism and respiration O53937 GO:0016491,GO:0055114 1.-.-.- O53937 ML1541,ML1541c MMAR_2667 Mb1814 NC_000962.3 Mycobrowser_v4 CDS 2017740 2019260 . + 0 Rv1782 eccB5 Function unknown ESX conserved component EccB5. ESX-5 type VII secretion system protein. Probable membrane protein. Rv1782, (MTV049.04), len: 506 aa. eccB5, esx conserved component, ESX-5 type VII secretion system protein, probable membrane protein, similar to four other Mycobacterium tuberculosis hypothetical membrane proteins e.g. O05449|Rv3895c|MTCY15F10.17|Z94121 (495 aa), FASTA scores: opt: 1106, E(): 0, (41.2% identity in 485 aa overlap); Rv0283, Rv3450c, and Rv3869, all located near ESAT-6 family genes. Also similar to O33088|MLCB628.17C|Y14967 cosmid B628 from Mycobacterium leprae (481 aa), (32.7% identity in 486 aa overlap); and equivalent to Q9Z5I3|MLCB596.27|AL035472 hypothetical protein from Mycobacterium leprae (506 aa) (82.6% identity in 506 aa overlap). Has hydrophobic stretch from aa 54-76. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WNQ9 No cell wall and cell processes O53933 P9WNQ9 GO:0005886,GO:0016021 P9WNQ9 ML1544c MMAR_2664 Mb1811 NC_000962.3 Mycobrowser_v4 CDS 2019257 2023432 . + 0 Rv1783 eccC5 Function unknown ESX conserved component EccC5. ESX-5 type VII secretion system protein. Rv1783, (MTV049.05-MTV049.06), len: 1391 aa. eccC5, esx conserved component, ESX-5 type VII secretion system protein, probable membrane protein. FtsK/SpoIIIE family protein. Similar to Rv3894c. Member of family of Mycobacterium tuberculosis hypothetical proteins including Rv3447c, Rv0284, Rv3870, Rv1783, Rv3871, Rv3894c, all linked to ESAT-6 family genes. Equivalent to Mycobacterium leprae hypothetical protein Q9Z512|MLCB596.28|AL035472 (1345 aa). Previously annotated as two separate genes eccCa5|Rv1783 and eccCb5|Rv1784, now fused due to A:T correction at position 2020563 resulting in *463L. Contains two times PS00017 ATP/GTP-binding site motif A (P-loop). Former Rv1784 - Predicted to be an outer membrane protein (See Song et al., 2008). P9WNA5 No cell wall and cell processes O53935,O53934 P9WNA5 GO:0005886,GO:0016021,GO:0003677,GO:0007049,GO:0051301,GO:0007059,GO:0005737,GO:0017111,GO:0005524 P9WNA5 ML1543c Mb1812 NC_000962.3 Mycobrowser_v4 CDS 2023447 2024628 . - 0 Rv1785c cyp143 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Probable cytochrome P450 143 Cyp143 Rv1785c, (MT1834, MTV049.07c), len: 393 aa. Probable cyp143, cytochrome P450 (1.14.-.-), similar to many e.g. AE0001|RZAE000101_4 Rhizobium sp. NGR234 (414 aa), FASTA scores: opt: 663, E(): 0, (32.4% identity in 413 aa overlap). Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. Belongs to the cytochrome P450 family. P9WPL3 No intermediary metabolism and respiration P63723 P9WPL3 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPL3 ML1542 MMAR_2666 Mb1813c NC_000962.3 Mycobrowser_v4 CDS 2025301 2026398 . + 0 Rv1787 PPE25 Function unknown PPE family protein PPE25 Rv1787, (MTV049.09), len: 365 aa. PPE25, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, similar to Z74024|MTCY274.24 Mycobacterium tuberculosis cosmid (404 aa), FASTA scores: opt: 837, E(): 0, (52.0% identity in 406 aa overlap). P9WI13 No PE/PPE Q79FK8 P9WI13 P9WI13 Mb1815 NC_000962.3 Mycobrowser_v4 CDS 2026790 2027971 . + 0 Rv1789 PPE26 Function unknown PPE family protein PPE26 Rv1789, (MTV049.11), len: 393 aa. PPE26, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, highly similar to others e.g.Z98268|MTCI125.26 Mycobacterium tuberculosis cosmid (385 aa), FASTA score: opt: 1283, E(): 0, (62.7% identity in 408 aa overlap). Q79FK6 No PE/PPE Q79FK6 Q79FK6 Q79FK6 Mb1817 NC_000962.3 Mycobrowser_v4 CDS 2030347 2030643 . + 0 Rv1792 esxM Function unknown ESAT-6 like protein EsxM Rv1792, (MTV049.14), len: 98 aa. EsxM, ESAT-6 like protein (see Gey Van Pittius et al., 2001), member of Mycobacterium tuberculosis QILSS family of proteins with Rv1038c, Rv1197, Rv3620c and Rv2347c. Has in-frame stop codon at 18074, no error could be found to account for this. Identical (apart from stop codon) to P96363|Rv1038c|MTCY10G2.11 putative ESAT-6 like protein 2 (98 aa), FASTA scores: opt: 389, E(): 5.8e-26, (100.0% identity in 58 aa overlap). Similar protein present in Mycobacterium leprae e.g. Q49946|MLCB1701.06C|AL049191 putative ESAT-6 like protein X (95 aa), FASTA scores: opt: 343, E(): 1.6e-17, (57.6% identity in 92 aa overlap). Seems to belong to the ESAT6 family. Yes cell wall and cell processes Mb1820 NC_000962.3 Mycobrowser_v4 CDS 2029904 2030203 . + 0 Rv1791 PE19 Function unknown PE family protein PE19 Rv1791, (MTV049.13), len: 99 aa. PE19, Member of the Mycobacterium tuberculosis PE family, but no glycine rich C-terminus (see Brennan & Delogu 2002), highly similar to Z93777|MTCI364.07 M.tuberculosis cosmid (99 aa) opt: 430 E(): 2.4e-21, (75.5% identity in 98 aa overlap). Q79FK4 No PE/PPE Q79FK4 Q79FK4 Q79FK4 Mb1819 NC_000962.3 Mycobrowser_v4 CDS 2030694 2030978 . + 0 Rv1793 esxN Function unknown Putative ESAT-6 like protein EsxN (ESAT-6 like protein 5) Rv1793, (MT1842, MTV049.15), len: 94 aa. EsxN, ESAT-6 like protein (see citation below), almost identical to several mycobacterial proteins of the ESAT-6-like family including P95242|Rv2346c|MTCY98.15C|Z83860 putative ESAT-6 like protein 6 (94 aa), FASTA scores: opt: 610, E(): 0, (97.9 % identity in 94 aa overlap); Rv3619c, Rv1037c, and Rv1198, etc. Also present in Mycobacterium leprae. Seems to belong to the ESAT6 family. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WNJ3 No cell wall and cell processes P9WNJ3 P9WNJ3 MMAR_3662,MMAR_5119 Mb1821 NC_000962.3 Mycobrowser_v4 CDS 2032240 2033751 . + 0 Rv1795 eccD5 Unknown ESX conserved component EccD5. ESX-5 type VII secretion system protein. Probable membrane protein. Rv1795, (MTV049.17), len: 503 aa. eccD5, esx conserved component, ESX-5 type VII secretion system protein, probable membrane protein, has a hydrophilic stretch from ~1-130 then very hydrophobic. Similar to several other mycobacterial proteins, all linked to ESAT-6 family e.g. Rv3887c|MTY15F10.24|Z94121 (509 aa), FASTA scores: opt: 360, E(): 1.6e-15, (26.7% identity in 514 aa overlap); Rv3448, and Rv0290. P9WNP9 No cell wall and cell processes O53944 P9WNP9 GO:0005886,GO:0016021 ML1539c MMAR_2677 Mb1823 NC_000962.3 Mycobrowser_v4 CDS 2057528 2058193 . + 0 Rv1815 Rv1815 Function unknown Conserved protein Rv1815, (MTCY1A11.28c), len: 221 aa. Conserved protein, similar to G473456 hypothetical protein from Mycobacterium fortuitum (255 aa), FASTA scores: opt: 182, E(): 3.2e-05, (29.6% identity in 230 aa overlap). Alternative nucleotide at position 2057774 (a->T; I83F) has been observed. Predicted to be an outer membrane protein (See Song et al., 2008). P9WLR9 No conserved hypotheticals P9WLR9 GO:0003824 P9WLR9 ML0932 MMAR_2692 MSMEG_3661 Mb1845 NC_000962.3 Mycobrowser_v4 CDS 2039159 2039350 . + 0 Rv1799 lppT Unknown Probable lipoprotein LppT Rv1799, (MTV049.21), len: 63 aa. Probable lppT lipoprotein, has possible signal peptide and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O53948 No cell wall and cell processes O53948 Mb1827 NC_000962.3 Mycobrowser_v4 CDS 2039453 2041420 . + 0 Rv1800 PPE28 Function unknown PPE family protein PPE28 Rv1800, (MTV049.22), len: 655 aa. PPE28, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, C-terminal very similar to parts of PE proteins e.g. Z92770|MTCI5.25|Rv0151c (588 aa), FASTA scores: opt: 1269, E(): 0, (41.5% identity in 591 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). P9WI11 No PE/PPE Q79FK2 P9WI11 P9WI11 MMAR_1027 Mb1828 NC_000962.3 Mycobrowser_v4 CDS 2042001 2043272 . + 0 Rv1801 PPE29 Function unknown PPE family protein PPE29 Rv1801, (MTV049.23), len: 423 aa. PPE29, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, most similar to AL022021|MTV049.29|Rv1808 (409 aa), FASTA scores: opt: 1229, E(): 0, (55.2% identity in 422 aa overlap). P9WI09 No PE/PPE Q7D7X9 P9WI09 P9WI09 Mb1829 NC_000962.3 Mycobrowser_v4 CDS 2043384 2044775 . + 0 Rv1802 PPE30 Function unknown PPE family protein PPE30 Rv1802, (MTV049.24), len: 463 aa. PPE30, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, most similar to AL022021|MTV049.30|Rv1809 (468 aa), FASTA scores: opt: 1238, E(): 0, (51.0% identity in 471 aa overlap). P9WI07 No PE/PPE P0A692 P9WI07 P9WI07 MMAR_2928 Mb1830 NC_000962.3 Mycobrowser_v4 CDS 2049921 2051150 . + 0 Rv1808 PPE32 Function unknown PPE family protein PPE32 Rv1808, (MTV049.30), len: 409 aa. PPE32, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, most similar to Rv1800|MTV049.22|AL022021 (655 aa), FASTA scores: opt: 1225, E(): 0, (55.1% identity in 423 aa overlap). Contains PS00343 Gram-positive cocci surface proteins 'anchoring' hexapeptide. Nucleotide position 2050913 in the genome sequence has been corrected, A:G resulting in E331E. P9WI05 No PE/PPE Q79FJ6 P9WI05 P9WI05 MMAR_1461,MMAR_2684 Mb1837 NC_000962.3 Mycobrowser_v4 CDS 2055681 2056112 . - 0 Rv1813c Rv1813c Function unknown Conserved hypothetical protein Rv1813c, (MTCY16F9.01), len: 143 aa. Conserved hypothetical protein. Possibly a exported protein with potential N-terminal signal sequence. Similar to Q11050|Rv1269c|MTCY50.13 hypothetical protein from Mycobacterium tuberculosis (124 aa), (42.7% identity in 143 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). Predicted possible vaccine candidate (See Zvi et al., 2008). P9WLS1 No conserved hypotheticals P9WLS1 MMAR_1426,MMAR_2533 Mb1843c NC_000962.3 Mycobrowser_v4 CDS 2047023 2047349 . - 0 Rv1804c Rv1804c Function unknown Conserved protein Rv1804c, (MTV049.26c), len: 108 aa. Conserved protein, similar to several hypothetical Mycobacterium tuberculosis proteins that may be exported (hydrophobic stretch at N-terminus) e.g. O07222|Rv1810|MTCY16F9.04C|Z96073 (118 aa), FASTA scores: opt: 361, E(): 2.3e-19, (53.5% identity in 101 aa overlap); Rv0622, Rv1690, and Rv3067, etc. Predicted to be an outer membrane protein (See Song et al., 2008). O53953 No conserved hypotheticals O53953 O53953 Mb1833c NC_000962.3 Mycobrowser_v4 CDS 2047687 2048034 . - 0 Rv1805c Rv1805c Unknown Hypothetical protein Rv1805c, (MTV049.27c), len: 115 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53954 No conserved hypotheticals O53954 ML1535 Mb1834c NC_000962.3 Mycobrowser_v4 CDS 2048072 2048371 . + 0 Rv1806 PE20 Function unknown PE family protein PE20 Rv1806, (MTV049.28), len: 99 aa. PE20, Member of the Mycobacterium tuberculosis PE family of gly-, ala-rich proteins (see citation below), most similar to Rv1788|MTV049.10|AL022021 (99 aa), FASTA scores: opt: 334, E(): 4.7 e-15, (59.8% identity in 97 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L7N656 No PE/PPE Q7D7X6 L7N656 L7N656 ML1534,ML1534c Mb1835 NC_000962.3 Mycobrowser_v4 CDS 2052933 2053289 . + 0 Rv1810 Rv1810 Function unknown Conserved protein Rv1810, (MTCY16F9.04c), len: 118 aa. Conserved protein, similar to several hypothetical Mycobacterium tuberculosis proteins that may be exported (possible N-terminal signal sequence) e.g. O53953|Rv1804c|MTV049.26c|AL022021 (108 aa), FASTA scores: opt: 361, E(): 9.6e-17, (53.5% identity in 101 aa overlap); Rv0622, and Rv1690, etc. O07222 No conserved hypotheticals O07222 O07222 O07222 Mb1840 NC_000962.3 Mycobrowser_v4 CDS 2053443 2054147 . + 0 Rv1811 mgtC Thought to be involved in Mg2+ transport (import). May act as an accessory protein for MGTB so mediating magnesium influx into the cytosol [catalytic activity: ATP + H(2)O + Mg(2+)(out) = ADP + phosphate + Mg(2+)(in)]. Possible Mg2+ transport P-type ATPase C MgtC Rv1811, (MTCY16F9.03c), len: 234 aa. Possible mgtC, magnesium (Mg2+) transport P-type ATPase C (transmembrane protein), highly similar to many e.g. NP_442124.1|NC_000911 Mg2+ transport ATPase from Synechocystis sp. strain PCC 6803 (234 aa); NP_251248.1|NC_002516 probable transport protein from Pseudomonas aeruginosa (230 aa); P22037|ATMC_SALTY|STM3764 magnesium transport ATPase protein C from Salmonella typhimurium (231 aa), FASTA scores: opt: 545, E(): 4.1e-30, (42.3% identity in 220 aa overlap); N-terminus of NP_213315.1|NC_000918 Mg(2+) transport ATPase from Aquifex aeolicus (225 aa); etc. Belongs to the MGTC / SAPB family I6YBN6 No cell wall and cell processes O07221 I6YBN6 GO:0004012,GO:0016020 3.6.3.1 I6YBN6 ML0929 MMAR_2687 Mb1841 NC_000962.3 Mycobrowser_v4 CDS 2054157 2055359 . - 0 Rv1812c Rv1812c Function unknown; probably involved in cellular metabolism Probable dehydrogenase Rv1812c, (MTCY16F9.02), len: 400 aa. Probable dehydrogenase, similar to other dehydrogenases/oxidases e.g. AE001947|AE001947_10 NADH dehydrogenase II of Deinococcus radiodurans (379 aa), FASTA scores: opt: 404, E(): 3.4e-18, (26.4% identity in 363 aa overlap) and DHNA_HAEIN|P44856 nadh dehydrogenase (444 aa), FASTA scores: opt: 200, E(): 8.5e-06, (23.3% identity in 258 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical dehydrogenases Rv0392c, and Rv1854c|MTCY359.19 ndh probable NADH dehydrogenase (31.5% identity in 321 aa overlap). P9WJJ1 No intermediary metabolism and respiration O07220 P9WJJ1 GO:0055114,GO:0016491,GO:0050660 1.6.-.- P9WJJ1 ML0930,ML0930c MMAR_2688 Mb1842c NC_000962.3 Mycobrowser_v4 CDS 2058256 2058960 . + 0 Rv1816 Rv1816 Involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv1816, (MTCY1A11.27c), len: 234 aa. Possible transcriptional regulatory protein. MEME analysis suggests similarity to putative Mycobacterium tuberculosis transcriptional regulators, Rv0653c, Rv0681. Contains helix-turn-helix motif at aa 38-59 (+4.30 SD). P9WMC9 No regulatory proteins P67438 P9WMC9 GO:0006355,GO:0006350,GO:0003700,GO:0016021 P9WMC9 ML0933 MSMEG_3659 Mb1846,Mb1847 NC_000962.3 Mycobrowser_v4 CDS 2059595 2061058 . + 0 Rv1817 Rv1817 Function unknown; probably involved in cellular metabolism Possible flavoprotein Rv1817, (MTCY1A11.26c), len: 487 aa. Possible flavoprotein, similar to G746486 flavoprotein subunit of fumarate reductase FAD domain homologue (474 aa), FASTA scores: opt: 223, E(): 5.7e-07, (24.1% identity in 489 aa overlap); and AJ236923|SFR236923_3 soluble fumarate reductase of Shewanella frigidimarina ifcA (588 aa), FASTA scores: opt: 310, E(): 2.5e-11, (27.3% identity in 484 aa overlap). Q50616 No intermediary metabolism and respiration Q50616 Q50616 GO:0016491,GO:0009055 Q50616 ML0934 MMAR_2694 MSMEG_3657 Mb1848 NC_000962.3 Mycobrowser_v4 CDS 2061178 2062674 . - 0 Rv1818c PE_PGRS33 Function unknown. Seems to influence both cell surface interactions among mycobacteria and the interactions of bacteria with macrophages. PE-PGRS family protein PE_PGRS33 Rv1818c, (MTCY1A11.25), len: 498 aa. PE_PGRS33, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins, similar to many. Contains 2 x PS00583 pfkB family of carbohydrate kinases signature 1. Supposedly localised to the cell surface (see citations below). P9WIF5 No PE/PPE Q50615 P9WIF5 GO:0005886,GO:0016021 P9WIF5 MMAR_0478,MMAR_0608 Mb1849c NC_000962.3 Mycobrowser_v4 CDS 2064799 2066442 . + 0 Rv1820 ilvG Valine and isoleucine biosynthesis (first step) [catalytic activity: 2-acetolactate + CO(2) = 2 pyruvate] Probable acetolactate synthase IlvG (acetohydroxy-acid synthase)(ALS) Rv1820, (MTCY1A11.23c), len: 547 aa. Probable ilvG, acetolactate synthase. Equivalent to AL008609|MLCB1788.46c ilvG from Mycobacterium leprae (548 aa) (86.1% identity in 548 aa overlap). Similar to ILVB_KLEPN|P27696 (559 aa), FASTA scores: opt: 660, E(): 2.9e-34, (29.1% identity in 549 aa overlap). Also similar to other Mycobacterium tuberculosis Ilv proteins e.g. Rv3003c (ilvB), etc. Contains PS00187 Thiamine pyrophosphate enzymes signature. P9WG39 No intermediary metabolism and respiration P66946 P9WG39 GO:0009082,GO:0000287,GO:0030976,GO:0003984 2.2.1.6 P9WG39 ML2083,ML2083c MMAR_2697 Mb1851 NC_000962.3 Mycobrowser_v4 CDS 2096183 2096599 . - 0 Rv1846c blaI Involved in transcriptional mechanism Transcriptional repressor BlaI Rv1846c, (MTCY359.27), len: 138 aa. BlaI, transcriptional repressor. Equivalent to MLCB1788.17|AL008609 hypothetical protein from Mycobacterium leprae (142 aa), FASTA scores: opt: 736 E(): 0, (95.1% identity in 123 aa overlap). Also similar to BLAI_BACLI|P06555 penicillinase repressor (128 aa), fasta scores: opt: 114, E(): 0.12, (23.7% identity in 131 aa overlap). P9WMJ5 No regulatory proteins 2G9W P95163 P9WMJ5 GO:0005737,GO:0016481,GO:0046677,GO:0016566,GO:0006350,GO:0003677 P9WMJ5 ML2063 MMAR_2720 MSMEG_3630 Mb1877c NC_000962.3 Mycobrowser_v4 CDS 2099694 2100329 . + 0 Rv1851 ureF Probably facilitates nickel incorporation Urease accessory protein UreF Rv1851, (MTCY359.22c), len: 211 aa. UreF, urease accessory protein. Identical to UREF_MYCTU|P50050 from M. tuberculosis. P9WFE5 No intermediary metabolism and respiration P50050 P9WFE5 GO:0016151,GO:0006807,GO:0005737 P9WFE5 MMAR_2725 MSMEG_3624 Mb1882 NC_000962.3 Mycobrowser_v4 CDS 2069080 2069709 . + 0 Rv1822 pgsA2 Thought to be involved in cardiolipin biosynthesis; generates cardiolipin from phosphatidylglycerol and CDP-diacylglycerol [catalytic activity: may be: phosphatidylglycerol + phosphatidylglycerol -> cardiolipin + glycerol, or: CDP-diacylglycerol + glycerol 3-phosphate = CMP + 3-(3-phosphatidyl)-glycerol 1-phosphate]. Probable CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase PgsA2 (PGP synthase) (phosphatidylglycerophosphate synthase) (3-phosphatidyl-1'-glycerol-3'phosphate synthase) Rv1822, (MTCY1A11.21c), len: 209 aa. Probable pgsA2, CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyl-transferase (see citation below), integral membrane protein, equivalent to AL008609|MLCB1788_17 phosphatidyltransferase from Mycobacterium leprae (206 aa), FASTA score: (76.6% identity in 205 aa overlap). Also highly similar or similar to others e.g. CAB88885.1|AL353861 putative CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyl-transferase from Streptomyces coelicolor (215 aa); AAC44003.1|U29587 phosphatidylglycerol phosphate synthase from Rhodobacter sphaeroides (227 aa); NP_405431.1|NC_003143 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase from Yersinia pestis (182 aa); P06978|PGSA_ECOLI CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase from Escherichia coli (181 aa), FASTA scores: opt: 252, E(): 2.8e-09, (29.7% identity in 175 aa overlap); etc. Also similar to Rv2746c|PGSA3|MTV002.11c CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (PGP synthase) from Mycobacterium tuberculosis (209 aa). Contains PS00379 CDP-alcohol phosphatidyltransferases signature; and PS00075 Dihydrofolate reductase signature. Belongs to the CDP-alcohol phosphatidyltransferase class-I family. P9WPG5 No lipid metabolism P63753 P9WPG5 GO:0016021,GO:0008654,GO:0005886,GO:0008444 2.7.8.5 P9WPG5 ML2081,ML2081c MMAR_2699 MSMEG_3652 Mb1853 NC_000962.3 Mycobrowser_v4 CDS 2069702 2070625 . + 0 Rv1823 Rv1823 Function unknown Conserved protein Rv1823, (MTCY01A11.20), len: 307 aa. Conserved protein, similar to P71582|MTCY10H4.12|RV0012 hypothetical protein CY10H4.12 from Mycobacterium tuberculosis (262 aa), FASTA scores: opt: 304, E(): 1.5e-12, (30.1% identity in 246 aa overlap). P9WFG1 No conserved hypotheticals P64891 P9WFG1 GO:0005886,GO:0016021 P9WFG1 ML2080,ML2080c MMAR_2700 MSMEG_3651 Mb1854 NC_000962.3 Mycobrowser_v4 CDS 2070654 2071019 . + 0 Rv1824 Rv1824 Unknown Conserved hypothetical membrane protein Rv1824, (MTCY1A11.19c), len: 121 aa. Conserved hypothetical membrane protein similar to P28265|SBP_BACSU sbp protein from Bacillus subtilis (121 aa), FASTA scores: opt: 261, E(): 1.9e-12, (38.9% identity in 113 aa overlap). P9WLR7 No cell wall and cell processes P64893 P9WLR7 GO:0005886,GO:0016021 ML2079,ML2079c MMAR_2701 MSMEG_3650 Mb1855 NC_000962.3 Mycobrowser_v4 CDS 2071036 2071914 . + 0 Rv1825 Rv1825 Function unknown Conserved protein Rv1825, (MTCY1A11.18c), len: 292 aa. Conserved protein, weak similarity to Mycobacterium tuberculosis hypothetical proteins Q50610|MTCY1A11.20C|Rv1823|Z78020 (307 aa), FASTA scores: opt: 182, E(): 0.00044, (29.9% identity in 204 aa overlap); and Rv0012. Has a hydrophobic stretch, TMhelix from aa 67 to 85. P9WFG3 No conserved hypotheticals 3GMG P64895 P9WFG3 GO:0005886,GO:0016021 P9WFG3 ML2078,ML2078c MMAR_2702 MSMEG_3649 Mb1856 NC_000962.3 Mycobrowser_v4 CDS 2074841 2075518 . + 0 Rv1830 Rv1830 Function unknown Conserved hypothetical protein Rv1830, (MTCY1A11.13c), len: 225 aa. Conserved hypothetical protein, equivalent to Mycobacterium leprae hypothetical protein MLCB1788.33c|AL008609|O32916 (231 aa), FASTA scores: opt: 1307, E(): 0, (89.6% identity in 231 aa overlap). P9WME5 No conserved hypotheticals P9WME5 GO:0006355,GO:0006350,GO:0003700,GO:0000166 P9WME5 ML2073,ML2073c MMAR_2707 MSMEG_3644 Mb1861 NC_000962.3 Mycobrowser_v4 CDS 2157382 2157987 . - 0 Rv1911c lppC Unknown Probable lipoprotein LppC Rv1911c, (MTCY180.07), len: 201 aa. Probable lipoprotein lppC, contains appropriately positioned prokaryotic membrane lipoprotein lipid attachment site (PS00013). Very similar to downstream ORF MTCY180.08 (204 aa) (although this lacks lipoprotein motif), FASTA score: opt: 831, E(): 0, (64.0% identity in 200 aa overlap). Also similar to Q9Z729|Y877_CHLPN hypothetical protein CPN0877 from Chlamydia pneumoniae (strain CWL029) (150 aa). P9WFN3 No cell wall and cell processes P67224 P9WFN3 P9WFN3 MSMEG_6851 Mb1946c NC_000962.3 Mycobrowser_v4 CDS 2546883 2547752 . + 0 Rv2275 Rv2275 Unknown Conserved hypothetical protein Rv2275, (MTCY339.35c), len: 289 aa. Conserved hypothetical protein. Some similarity to Bacillus subtilis sp|O34351|O34351 YVMC (248 aa), FASTA score: opt: 280, E(): 2.7e -11; 28.2% identity in 227 aa overlap P9WPF9 No conserved hypotheticals 2X9Q P9WPF9 P9WPF9 Mb2298 NC_000962.3 Mycobrowser_v4 CDS 2072596 2073084 . + 0 Rv1827 garA Function unknown Conserved protein with FHA domain, GarA Rv1827, (MTCY1A11.16c), len: 162 aa. GarA, conserved protein with forkhead-associated domain at C-terminus (see citation below), equivalent to O32919|MLCB1788.36c hypothetical protein from Mycobacterium leprae (162 aa), FASTA scores: opt: 888, E(): 0, (87.0% identity in 161 aa overlap). Putative physiological substrate of PknB and PknG. P9WJA9 No conserved hypotheticals 2KFU P64897 P9WJA9 P9WJA9 ML2076,ML2076c MMAR_2704 MSMEG_3647 Mb1858 NC_000962.3 Mycobrowser_v4 CDS 2073081 2073824 . + 0 Rv1828 Rv1828 Function unknown Conserved protein Rv1828, (MTCY1A11.15c), len: 247 aa. Conserved protein, equivalent to O32918|MLCB1788.35c|AL008609 hypothetical protein from Mycobacterium leprae (251 aa), FASTA scores: opt: 1397, E(): 0, (87.6% identity in 251 aa overlap). P9WME7 No conserved hypotheticals P67669 P9WME7 GO:0006355,GO:0006350,GO:0003700,GO:0000166 P9WME7 ML2075,ML2075c MMAR_2705 MSMEG_3646 Mb1859 NC_000962.3 Mycobrowser_v4 CDS 2078929 2079789 . - 0 Rv1833c Rv1833c May act on a wide range of 1-haloalkanes, haloalcohols, haloalkenes and some haloaromatic compounds [catalytic activity: 1-haloalkane + H(2)O <=> a primary alcohol + halide] Possible haloalkane dehalogenase Rv1833c, (MTCY1A11.10), len: 286 aa. Possible haloalkane dehalogenase. Similar to several haloalkane dehalogenase e.g. CAB45532.1|AJ243259 from Mycobacterium bovis (300 aa); also similar to LINB_PSEPA|P51698 1,3,4,6-tetrachloro-1,4-cyclohexadien from Pseudomonas paucimobilis (295 aa), FASTA scores: opt: 314, E(): 1.5e-13, (33.1% identity in 281 aa overlap). P9WMS1 No intermediary metabolism and respiration P64303 P9WMS1 GO:0018786 3.8.1.5 P9WMS1 ML2071 MMAR_2710 Mb1864c NC_000962.3 Mycobrowser_v4 CDS 2079830 2080696 . + 0 Rv1834 lipZ Unknown Probable hydrolase Rv1834, (MTCY1A11.09c), len: 288 aa. Probable lipZ, hydrolase, some similarity to haloalkane dehalogenases and D16262 hypothetical 38.9 kDa protein (335 aa), FASTA scores: opt: 507, E(): 7.6e-28, (33.0% identity in 300 aa overlap). P9WLR1 No intermediary metabolism and respiration Q50599 P9WLR1 GO:0003824 P9WLR1 MMAR_2711 MSMEG_2777 Mb1865 NC_000962.3 Mycobrowser_v4 CDS 2080701 2082587 . - 0 Rv1835c Rv1835c Function unknown Conserved hypothetical protein Rv1835c, (MTCY1A11.08), len: 628 aa. Conserved hypothetical protein, some similarity to putative acylases e.g. G216374 glutaryl 7-aca acylase precursor (634 aa) FASTA scores, opt: 202, E(): 3.5e-06, (25.1% identity in 669 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins Rv2800 and Rv1215c. P9WIQ9 No conserved hypotheticals P0A5F5 P9WIQ9 GO:0008152,GO:0008239 P9WIQ9 Mb1866c NC_000962.3 Mycobrowser_v4 CDS 2134273 2134872 . - 0 Rv1885c Rv1885c Involved in the shikimate pathway. Converts chorismate to prephenate in the biosynthesis of tyrosine and phenylalanine. Chorismate mutase Rv1885c, (MTCY180.33), len: 199 aa. Chorismate mutase, AroQ class (See Prakash et al., 2005, Sasso et al., 2005), some similarity to P42517|CHMU_ERWHE monofunctional chorismate mutase (181 aa), FASTA score: opt: 181, E(): 0.00017, (28.6% identity in 133 aa overlap). Contains N-terminal signal sequence. P9WIB9 No intermediary metabolism and respiration 2AO2,2F6L,2FP1,2FP2 O07746 P9WIB9 GO:0046417 P9WIB9 ML2029 MMAR_2773 MSMEG_2111 Mb1917c NC_000962.3 Mycobrowser_v4 CDS 2084756 2086981 . - 0 Rv1837c glcB Involved in glyoxylate bypass (second step), an alternative to the tricarboxylic acid cycle [catalytic activity: L-malate + CoA = acetyl-CoA + H(2)O + glyoxylate] Malate synthase G GlcB Rv1837c, (MTCY1A11.06), len: 741 aa. glcB, malate synthase G (see citations below), highly similar to MASY_CORGL|P42450 malate synthase (738 aa), FASTA score: opt: 2961, E(): 0, (61.3% identity in 724 aa overlap). Belongs to the malate synthase G family. P9WK17 No intermediary metabolism and respiration 1N8I,1N8W,2GQ3 P0A5J4 P9WK17 GO:0006097,GO:0004474,GO:0006099,GO:0005737 2.3.3.9 P9WK17 ML2069 MMAR_2713 MSMEG_3640 Mb1868c NC_000962.3 Mycobrowser_v4 CDS 2087257 2087652 . - 0 Rv1838c vapC13 Unknown Possible toxin VapC13 Rv1838c, (MTCY359.35), len: 131 aa. Possible vapC13, toxin, part of toxin-antitoxin (TA) operon with Rv1839c, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Part of 14-membered Mycobacterium tuberculosis protein family with Rv2863|MTV003.09|AL008883 (126 aa), FASTA scores: opt: 293, E(): 1.5e-14, (38.2% identity in 123 aa overlap); Rv0749, Rv0277c, Rv2530c, etc. Also similar to AJ248288|CNSPAX06_181 Pyrococcus abyssi complete genome (136 aa), FASTA scores: opt: 197, E(): 2.2e-07, (33. 1% identity in 133 aa overlap). P9WFA1 No virulence, detoxification, adaptation P64901 P9WFA1 P9WFA1 Mb1869c NC_000962.3 Mycobrowser_v4 CDS 2087649 2087912 . - 0 Rv1839c vapB13 Unknown Possible antitoxin VapB13 Rv1839c, (MTCY359.34), len: 87 aa. Possible vapB13, antitoxin, part of toxin-antitoxin (TA) operon with Rv1838c (See Arcus et al., 2005; Pandey and Gerdes, 2005). Some similarity to others in M. tuberculosis e.g. Rv0239, Rv0662c P9WJ51 No virulence, detoxification, adaptation P9WJ51 GO:0003677 Mb1870c NC_000962.3 Mycobrowser_v4 CDS 2100340 2101014 . + 0 Rv1852 ureG Probably facilitates nickel incorporation Urease accessory protein UreG Rv1852, (MTCY359.21c), len: 224 aa. UreG, urease accessory protein. Identical to UREG_MYCTU|P50051 from M. tuberculosis. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the UreG family. P9WFE3 No intermediary metabolism and respiration P0A664 P9WFE3 GO:0005525,GO:0005737,GO:0016151,GO:0005524 P9WFE3 MMAR_2726 MSMEG_3623 Mb1883 NC_000962.3 Mycobrowser_v4 CDS 2110591 2111361 . - 0 Rv1863c Rv1863c Unknown Probable conserved integral membrane protein Rv1863c, (MTCY359.10), len: 256 aa. Probable conserved integral membrane protein, similar to Rv0804|Z95618|MTCY7H7A.05 Hypothetical protein from Mycobacterium tuberculosis (209 aa), FASTA scores: opt: 199, E(): 1e-06, (33.2% identity in 220 aa overlap); and Rv0658c. P95152 No cell wall and cell processes P95152 P95152 GO:0016020 P95152 ML2052 MMAR_2740 MSMEG_3612 Mb1894c NC_000962.3 Mycobrowser_v4 CDS 2225413 2225832 . - 0 Rv1982c vapC36 Unknown Possible toxin VapC36. Contains PIN domain. Rv1982c, (MTCY39.37), len: 139 aa. Possible vapC36, toxin, part of toxin-antitoxin (TA) operon with Rv1982A, contains PIN domain, see Arcus et al. 2005. belongs to the UPF0110 family. Similar to Rv0624|Z92772|MTY20H10.05 from Mycobacterium tuberculosis (131 aa), FASTA scores: opt: 288, E(): 4.1e-14, (40.2% identity in 127 aa overlap); also similar to Rv0624, Rv2759c, and Rv0609 P9WF65 No virulence, detoxification, adaptation P0A652 P9WF65 P9WF65 Mb2004c NC_000962.3 Mycobrowser_v4 CDS 2089681 2090718 . - 0 Rv1841c Rv1841c Unknown Conserved hypothetical membrane protein Rv1841c, (MTCY359.32), len: 345 aa. Conserved hypothetical membrane protein. Some similarity to O07585|YHDP_BACSU hypothetical 49.9 kDa protein from Bacillus subtilis (444 aa), FASTA scores: opt: 620, E(): 0, (31.1% identity in 350 aa overlap). Also similar to other Mycobacterium tuberculosis proteins e.g. Rv1842c, Rv2366c. P9WLQ7 No cell wall and cell processes Q50593 P9WLQ7 GO:0005886,GO:0016021 P9WLQ7 ML2068 MMAR_2715 MSMEG_3638 Mb1872c NC_000962.3 Mycobrowser_v4 CDS 2092259 2093698 . - 0 Rv1843c guaB1 Involved in GMP biosynthesis [catalytic activity: inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH] Probable inosine-5'-monophosphate dehydrogenase GuaB1(imp dehydrogenase) (IMPDH) (IMPD) Rv1843c, (MTCY359.30), len: 479 aa. Probable guaB1, inosine-5'-monophosphate dehydrogenase. Similar to others e.g. IMDH_BACSU|P21879 from Bacillus subtilis (513 aa), FASTA score: opt: 904, E(): 0, (37.8% identity in 471 aa overlap). Similar to other Mycobacterium tuberculosis proteins e.g. guaB2, Rv3411c. P9WKI3 No intermediary metabolism and respiration P65172 P9WKI3 GO:0055114,GO:0003938 1.-.-.- P9WKI3 ML2066 MMAR_2717 MSMEG_3634 Mb1874c NC_000962.3 Mycobrowser_v4 CDS 2095218 2096168 . - 0 Rv1845c blaR Involved in transcriptional mechanism Possible sensor-transducer protein BlaR Rv1845c, (MTCY359.28), len: 316 aa. Possible blaR, sensor-transducer protein. Conserved hypothetical transmembrane protein. Equivalent to MLCB1788.18|AL008609 Hypothetical protein from Mycobacterium leprae (316 aa), FASTA scores: opt: 1762, E(): 0, (87.6% identity in 314 aa overlap). Similar to proteins in Streptomyces coelicolor e.g. SC10A7.04|AL078618.1. P95164 No cell wall and cell processes P95164 P95164 GO:0046872,GO:0004222,GO:0006508,GO:0016021 P95164 ML2064 MMAR_2719 MSMEG_3631 Mb1876c NC_000962.3 Mycobrowser_v4 CDS 2096877 2097299 . + 0 Rv1847 Rv1847 Function unknown Conserved protein Rv1847, (MTCY359.26c), len: 140 aa. Conserved protein, possible thioesterase, some similarity to YBDB proteins of Escherichia coli and H. influenzae e.g. P15050|YBDB_ECOLI hypothetical 15.0 KD protein in ENTA-CSTA intergenic region (137 aa), FASTA scores: opt: 232, E(): 6.6e-10, (35.8% identity in 106 aa overlap); C48956|G142208 thioesterase from Arthrobacter sp (151 aa), FASTA score: opt: 254, E(): 1.7e-11, (33.3% identity in 138 aa overlap). Also similar to AF064959|AF064959_1 hypothetical protein from Coxiella burnetii (148 aa), FASTA score: opt: 264, E(): 9.3e- 12, (36.8% identity in 117 aa overlap). P9WIM3 No conserved hypotheticals 3S4K P95162 P9WIM3 GO:0016787 3.1.2.- P9WIM3 ML2062,ML2062c MMAR_2721 MSMEG_3628 Mb1878 NC_000962.3 Mycobrowser_v4 CDS 2097348 2097650 . + 0 Rv1848 ureA Involved in the conversion of urea to NH3 [catalytic activity: urea + H2O = CO2 + 2 NH3] Urease gamma subunit UreA (urea amidohydrolase) Rv1848, (MTCY359.25c), len: 100 aa. UreA, urease gamma subunit. Similar to URE3_MYCTU|P50043 from Mycobacterium tuberculosis (100 aa), FASTA scores: opt: 630, E(): 1.3e-36, (99.0% identity in 100 aa overlap). Belongs to the urease gamma subunit family. P9WFE7 No intermediary metabolism and respiration 2FVH P0A676 P9WFE7 GO:0016151,GO:0019627,GO:0009039,GO:0005737 3.5.1.5 P9WFE7 MMAR_2722 MSMEG_3627 Mb1879 NC_000962.3 Mycobrowser_v4 CDS 2097647 2097961 . + 0 Rv1849 ureB Involved in the conversion of urea to NH3 [catalytic activity: urea + H2O = CO2 + 2 NH3] Urease beta subunit UreB (urea amidohydrolase) Rv1849, (MTCY359.24c), len: 104 aa. UreB, urease beta subunit. Identical to URE2_MYCTU|P50048 urease beta subunit from Mycobacterium tuberculosis (100 aa). Belongs to the urease gamma subunit family. P9WFE9 No intermediary metabolism and respiration P0A662 P9WFE9 GO:0016151,GO:0006807,GO:0009039,GO:0005737 3.5.1.5 P9WFE9 MMAR_2723 MSMEG_3626 Mb1880 NC_000962.3 Mycobrowser_v4 CDS 2101022 2101648 . + 0 Rv1853 ureD Probably facilitates nickel incorporation Probable urease accessory protein UreD Rv1853, (MTCY359.20c), len: 208 aa. UreD, probable urease accessory protein. Similar to URED_YEREN|P42868 Urease operon ureD protein from Yersinia enterocolitica (325 aa), Fasta scores: opt: 114, E(): 0.37, (25.2% identity in 119 aa overlap). P95161 No intermediary metabolism and respiration P95161 P95161 GO:0006807,GO:0016151 P95161 MMAR_2727 MSMEG_3622 Mb1884 NC_000962.3 Mycobrowser_v4 CDS 2103184 2104107 . - 0 Rv1855c Rv1855c Function unknown; probably involved in cellular metabolism Possible oxidoreductase Rv1855c, (MTCY359.18), len: 307 aa. Possible oxidoreductase, possibly a monooxygenase. Contains PS00217 Sugar transport proteins signature 2, probably fortuitously. Similar to G487716 (78-11) lincomycin production genes (29.2% identity in 154 aa overlap). Also similar to other Mycobacterium tuberculosis proteins e.g. Rv0953c, Rv0791c, Rv0132c, Rv2951c, etc. P95159 No intermediary metabolism and respiration P95159 P95159 GO:0016491,GO:0055114 1.-.-.- P95159 ML2060 MMAR_2729 MSMEG_3620 Mb1886c NC_000962.3 Mycobrowser_v4 CDS 2104146 2104823 . - 0 Rv1856c Rv1856c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv1856c, (MTCY359.17), len: 225 aa. Possible oxidoreductase. Equivalent to MLCB1788.11c|AL008609 oxidoreductase from Mycobacterium leprae (224 aa), FASTA scores: opt: 1211, E(): 0; (80.4% identity in 224 aa overlap). Some similarity to dehydrogenases of short-chain dehydrogenase/reductase family and fatty-acyl CoA reductases e.g. P16543|DHK2_STRVN granaticin polyketide synthase P (249 aa), FASTA score: opt: 194, E(): 1.1e-05, (32.5% identity in 237 aa overlap). P9WGQ1 No intermediary metabolism and respiration P95158 P9WGQ1 GO:0055114,GO:0016491 1.-.-.- P9WGQ1 ML2059 MMAR_2730 MSMEG_3619 Mb1887c NC_000962.3 Mycobrowser_v4 CDS 2104985 2105770 . + 0 Rv1857 modA Involved in the active transport of molybdenum into the cell across the membrane (import). Part of the binding-protein-dependent transport system modabc. Probable molybdate-binding lipoprotein ModA Rv1857, (MTCY359.16c), len: 261 aa. Probable modA, molybdate-binding protein attached to membrane by lipid-modified N-terminal cysteine (contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site), component of molybdate transport system (see citations below). Shows strong similarity to precursors of periplasmic molybdate/sulphate binding proteins e.g. O31229|Y10817|ANY108174 ModA from Arthrobacter nicotinovorans (260 aa), FASTA score: opt: 725, E(): 0, (47.8% identity in 249 aa overlap). P9WGU3 No cell wall and cell processes P0A5Y0 P9WGU3 GO:0015412,GO:0030288,GO:0005886,GO:0015689 P9WGU3 ML2058,ML2058c MMAR_2731 MSMEG_2016 Mb1888 NC_000962.3 Mycobrowser_v4 CDS 2109165 2109470 . + 0 Rv1861 Rv1861 Function unknown Probable conserved transmembrane protein Rv1861, (MTCY359.12c), len: 101 aa. Probable conserved transmembrane protein, showing weak similarity to AE002069|AE002069_10 hypothetical protein from Deinococcus radiodurans (146 aa), FASTA scores: opt: 154, E(): 0.0027, (30.8% identity in 104 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P95154 No cell wall and cell processes P95154 P95154 GO:0016021 ML2054,ML2054c MMAR_2738 MSMEG_3616 Mb1892 NC_000962.3 Mycobrowser_v4 CDS 2111354 2112109 . - 0 Rv1864c Rv1864c Function unknown Conserved protein Rv1864c, (MTCY359.09), len: 251 aa. Conserved protein. Similar to other hypothetical proteins e.g. AL031317|SC6G4.43 from Streptomyces coelicolor cosmid 6G (233 aa), FASTA scores: opt: 716, E(): 0, (54.4% identity in 215 aa overlap); also P43976|YIIM_HAEIN hypothetical protein hi0278 (221 aa), FASTA scores: opt: 223, E(): 3.8e-08, (29.5% identity in 173 aa overlap). P95151 No conserved hypotheticals P95151 P95151 GO:0030151,GO:0030170,GO:0003824 P95151 ML2051 MMAR_2741 MSMEG_3483 Mb1895c NC_000962.3 Mycobrowser_v4 CDS 2137519 2138079 . - 0 Rv1888c Rv1888c Unknown Possible transmembrane protein Rv1888c, (MTCY180.30), len: 186 aa. Possible transmembrane protein. O07744 No cell wall and cell processes O07744 GO:0016021 O07744 Mb1920c NC_000962.3 Mycobrowser_v4 CDS 2107736 2108713 . + 0 Rv1860 apa Unknown (could mediate bacterial attachment to host cells). Alanine and proline rich secreted protein Apa (fibronectin attachment protein) (immunogenic protein MPT32) (antigen MPT-32) (45-kDa glycoprotein) (45/47 kDa antigen) Rv1860, (MT1908, MTCY359.0013), len: 325 aa. Apa (alternate gene names: mpt32, modD), Ala-, Pro-rich 45/47 kDa secreted protein, very similar to P46842|N43L_MYCLE from Mycobacterium leprae (287 aa), FASTA scores: opt: 1166, E(): 0, (66.4% identity in 298 aa overlap). Known to be glycosylated fibronectin-binding protein (see some citations). Changes in the mannosylation pattern of this protein affect its ability to stimulate T-lymphocyte response. Major immunodominant antigen that has potential as a vaccine against tuberculosis. APA-ELISA could be used in diagnosis. P9WIR7 No cell wall and cell processes Q50906 P9WIR7 GO:0005576,GO:0050840 P9WIR7 ML2055,ML2055c MMAR_2737 MSMEG_3618 Mb1891 NC_000962.3 Mycobrowser_v4 CDS 2109544 2110584 . + 0 Rv1862 adhA Catalyzes the reversible oxidation of ethanol to acetaldehyde with the concomitant reduction of NAD Probable alcohol dehydrogenase AdhA Rv1862, (MTCY359.11), len: 346 aa. Probable adhA, alcohol dehydrogenase, similar to ADH2_BACST|P42327 alcohol dehydrogenase (339 aa), FASTA scores: opt: 630, E(): 2.4e-32 (34.4% identity in 320 aa overlap). Contains PS00059 Zinc-containing alcohol dehydrogenases signature. P9WQC1 No intermediary metabolism and respiration P95153 P9WQC1 GO:0055114,GO:0008270,GO:0004022 1.1.1.1 P9WQC1 ML2053,ML2053c MSMEG_3615 Mb1893 NC_000962.3 Mycobrowser_v4 CDS 2112106 2112966 . - 0 Rv1865c Rv1865c Function unknown; probably involved in cellular metabolism Probable short-chain type dehydrogenase Rv1865c, (MTCY359.08), len: 286 aa. Probable short-chain dehydrogenase, highly similar to C-terminus of NP_301650.1|NC_00267 putative oxidoreductase from Mycobacterium leprae (596 aa). Also similar to various dehydrogenases, generally belonging to short-chain family, e.g. AAG02168.1|AF212041_24|AF212041 3-oxoacyl-(acylcarrier protein) reductase from Zymomonas mobilis (251 aa); P50198|LINX_PSEPA 2,5-dichloro-2,5-cyclohexadiene-1,4-DIOL dehydrogenase from Sphingomonas paucimobilis (250 aa); NP_105680.1|NC_002678 sorbitol dehydrogenase (also similar to acetoin reductase) from Mesorhizobium loti (256 aa); etc. And highly similar to C-terminus of ephD|Rv2214c|MTCY190.25c from Mycobacterium tuberculosis (592 aa); and many other oxidoreductases from Mycobacterium tuberculosis e.g. Y00P_MYCTU|Q10402 putative oxidoreductase (650 aa), FASTA scores: opt: 439, E(): 8.9e-20, (32.5% identity in 280 aa overlap). Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. P95150 No intermediary metabolism and respiration P95150 P95150 GO:0055114,GO:0016491 1.-.-.- P95150 MMAR_2743 MSMEG_0771 Mb1896c NC_000962.3 Mycobrowser_v4 CDS 2145214 2146245 . - 0 Rv1899c lppD Unknown Possible lipoprotein LppD Rv1899c, (MTCY180.19), len: 343 aa. Possible lipoprotein; contains appropriately localized lipoprotein lipid attachment site (PS00013). Some similarity to C-terminal part of AE000717|AE000717_4 hypothetical protein from Aquifex aeolicus section 49 (165 aa), FASTA results: opt: 372, E(): 2.3e-14, (43.5% identity in 147 aa overlap); and Q44020 4-hydroxybutyrate dehydrogenase (173 aa), FASTA results: opt: 272, E(): 4.7e-09, (35.8% identity in 165 aa overlap). P9WK29 No cell wall and cell processes O07733 P9WK29 P9WK29 ML2017 Mb1934c NC_000962.3 Mycobrowser_v4 CDS 2579504 2579935 . - 0 Rv2307B Rv2307B Unknown Hypothetical glycine rich protein Rv2307B, len: 143 aa. Hypothetical unknown Gly- rich protein. Equivalent to AAK46653 from Mycobacterium tuberculosis strain CDC1551 (133 aa) but longer 10 aa. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q79FG2 No conserved hypotheticals Q79FG2 Mb2332c NC_000962.3 Mycobrowser_v4 CDS 2117347 2119446 . + 0 Rv1868 Rv1868 Function unknown Conserved hypothetical protein Rv1868, (MTCY359.05c), len: 699 aa. Conserved hypothetical protein, similar to products of three consecutive ORFS in Mycobacterium leprae MLCB2052.18|Z98604|B2052 (257 aa), FASTA scores: opt: 314, E(): 9.9e-12, (35.2% identity in 213 aa overlap); MLCB2052.17, and MLCB2052.16. Also similar to M. tuberculosis hypothetical protein Rv2047c. P95147 No conserved hypotheticals P95147 GO:0003824,GO:0008152 P95147 MMAR_2747 Mb1899 NC_000962.3 Mycobrowser_v4 CDS 2119460 2120695 . - 0 Rv1869c Rv1869c Function unknown; probably involved in cellular metabolism Probable reductase Rv1869c, (MTCY359.04), len: 411 aa. Probable reductase (1.-.-.-). Similar to several reductases e.g. CAC04223.1|AL391515 putative ferredoxin reductase from Streptomyces coelicolor (420 aa); THCD_RHOSO|P43494 rhodocoxin reductase (426 aa), FASTA scores: opt: 904, E(): 0, (40.8% identity in 370 aa overlap). Also similar to Mycobacterium tuberculosis proteins Rv0688 (406 aa) (39.9% identity in 391 aa overlap); and Rv0253 (nitrite reductase subunit). P95146 No intermediary metabolism and respiration P95146 P95146 GO:0055114,GO:0016491,GO:0050660 1.-.-.- P95146 ML2050 MSMEG_3594 Mb1900c NC_000962.3 Mycobrowser_v4 CDS 2120795 2121430 . - 0 Rv1870c Rv1870c Unknown Conserved hypothetical protein Rv1870c, (MTCY359.03), len: 211 aa. Conserved hypothetical protein. Some similarity to SC6F7.17c hypothetical protein from Streptomyces coelicolor (216 aa). P95145 No conserved hypotheticals P95145 P95145 ML2049 Mb1901c NC_000962.3 Mycobrowser_v4 CDS 2121495 2121884 . - 0 Rv1871c Rv1871c Function unknown Conserved protein Rv1871c, (MTCY359.02), len: 129 aa. Conserved protein, similar to Mycobacterium tuberculosis hypothetical proteins Q11057|Rv1261|MTCY50.21 (149 aa), FASTA score: opt: 125, E(): 0.019, (32.6% identity in 89 aa overlap); Rv0523c, and Rv1598c. P95144 No conserved hypotheticals P95144 P95144 P95144 ML2048 MMAR_2750 Mb1902c NC_000962.3 Mycobrowser_v4 CDS 2124381 2124824 . + 0 Rv1875 Rv1875 Function unknown Conserved protein Rv1875, (MTCY180.43c), len: 147 aa. Conserved protein. Some similarity to Mycobacterium tuberculosis hypothetical proteins e.g. Rv1155|MTCI65.22|Z95584 (147 aa), FASTA scores: opt: 178, E(): 7.4e-06, (26.9% identity in 130 aa overlap); Rv0121c and Rv2074. Also similar to AL079356|SC6G9.21 hypothetical protein from Streptomyces coelicolor (144 aa), FASTA scores: opt: 239, E(): 3.1 e-09, (38.7% identity in 137 aa overlap). O07754 No conserved hypotheticals O07754 O07754 GO:0010181 O07754 ML2039,ML2039c MMAR_2758 Mb1906 NC_000962.3 Mycobrowser_v4 CDS 2194970 2195347 . - 0 Rv1943c mazE5 Function unknown Possible antitoxin MazE5 Rv1943c, (MTCY09F9.21), len: 125 aa. Possible mazE5, antitoxin, part of toxin-antitoxin (TA) operon with Rv1942c (See Pandey and Gerdes, 2005; Zhu et al., 2006), shows some similarity with Rv1946c|MTCY09F9.18|lppG possible conserved lipoprotein from Mycobacterium tuberculosis (150 aa), FASTA score: (71.4% identity in 28 aa overlap). P9WJ89 No virulence, detoxification, adaptation P9WJ89 ML0330,ML0330c Mb1978c NC_000962.3 Mycobrowser_v4 CDS 2138444 2138617 . - 0 Rv1888A Rv1888A Function unknown Conserved hypothetical protein Rv1888A, len: 57 aa. Conserved hypothetical protein. Possibly continuation of Rv1889c, part of large family of Mycobacterium tuberculosis proteins with conserved N-terminal domain of ~ 120 aa. Includes: C-terminus of Rv0726c|P95074 conserved hypothetical protein (367 aa), FASTA scores: opt: 295, E(): 3.1e-15, (73.684% identity in 57 aa overlap); C-terminus of Rv3399|Q50726|MTCY78.29c conserved hypothetical protein (348 aa), FASTA scores: opt: 504, E(): 7.3e-29, (64.2% identity in 120 aa overlap); C-terminus of Rv0731c; etc. Q79FJ0 No conserved hypotheticals Q79FJ0 Q79FJ0 GO:0008168 Q79FJ0 Mb1921c NC_000962.3 Mycobrowser_v4 CDS 2128022 2129374 . + 0 Rv1878 glnA3 Involved in glutamine biosynthesis [catalytic activity: ATP + L-glutamate + NH(3) = ADP + glutamine + orthophosphate]. Probable glutamine synthetase GlnA3 (glutamine synthase) (GS-I) Rv1878, (MTCY180.40c), len: 450 aa. Probable glnA3, glutamine synthetase class I, similar to many e.g. GLNA_BACCE|P19064 from Bacillus cereus (443 aa), FASTA results: opt: 497, E(): 5.2e-23, (29.0% identity in 331 aa overlap); etc. Also similar to C-terminus of FLUG_EMENI|P38094 flug protein from emericella nidulans (865 aa), FASTA scores: opt: 227, E (): 6.4e-13, (29.9% identity in 394 aa overlap). Note that the downstream ORF MTCY180.39c is similar to the N-terminus. Also similar to three other potential glutamine synthases in M. tuberculosis: Q10378|GLN2_MYCTU|GLNA2|Rv2222c|MT2280|MTCY190.33c|MTCY427.03c; Rv2860c|MTV003.06c|glnA4 and Rv2220|glnA1. Belongs to the glutamine synthetase family. O07752 No intermediary metabolism and respiration O07752 O07752 GO:0006807,GO:0004356 6.3.1.2 O07752 ML2037,ML2037c MMAR_2763 MSMEG_3561 Mb1910 NC_000962.3 Mycobrowser_v4 CDS 2285628 2286530 . - 0 Rv2040c Rv2040c Thought to be involved in active transport of sugar across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable sugar-transport integral membrane protein ABC transporter Rv2040c, (MTV018.27c), len: 300 aa. Probable sugar-transport integral membrane protein ABC transporter (see citation below), similar to many. O53484 No cell wall and cell processes O53484 O53484 GO:0005886,GO:0006810,GO:0005215,GO:0016021 O53484 ML1426,ML1426c MMAR_3013 Mb2066c NC_000962.3 Mycobrowser_v4 CDS 2326944 2327501 . + 0 Rv2069 sigC Involved in promoter recognition, transcription initiation. RNA polymerase sigma factor, ECF subfamily, SigC Rv2069, (MTCY49.08), len: 185 aa. SigC, RNA polymerase sigma factor, ECF subfamily (see Gomez et al., 1997; Chen et al., 2000), similar to many. P9WGH1 No information pathways 2O7G,2O8X P66809 P9WGH1 GO:0016987,GO:0003700,GO:0006352,GO:0006355 P9WGH1 ML1448 MMAR_3051 Mb2095 NC_000962.3 Mycobrowser_v4 CDS 2580028 2580210 . - 0 Rv2307D Rv2307D Unknown Hypothetical protein Rv2307D, len: 60 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L7N683 No conserved hypotheticals L7N683 L7N683 Mb2333c NC_000962.3 Mycobrowser_v4 CDS 2131907 2132329 . - 0 Rv1881c lppE Unknown Possible conserved lipoprotein LppE Rv1881c, (MTCY180.37), len: 140 aa. Possible lppE, lipoprotein, showing some similarity to L12238|MSG18S19K_1 19K antigen from Mycobacterium intracellulare (162 aa), FASTA scores: opt: 137, E(): 0.0069, (27.6% identity in 156 aa overlap). Contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O07750 No cell wall and cell processes O07750 O07750 ML2032 MMAR_2769 Mb1913c NC_000962.3 Mycobrowser_v4 CDS 2133231 2133692 . - 0 Rv1883c Rv1883c Function unknown Conserved hypothetical protein Rv1883c, (MTCY180.35), len: 153 aa. Conserved hypothetical protein, some similarity to hypothetical proteins e.g. Rv2778c|AL008967|MTV002.43 from Mycobacterium tuberculosis (156 aa), FASTA score: opt: 212, E(): 3.1e-08, (34.4% identity in 151 aa overlap). Also similar to U75434|SAU75434_3 Nsh-OrfB from Streptomyces actuosus (173 aa), FASTA score: opt: 207, E(): 1.8e-07, (40.2% identity in 102 aa overlap). O07748 No conserved hypotheticals O07748 O07748 O07748 ML2031 MMAR_2771 Mb1915c NC_000962.3 Mycobrowser_v4 CDS 2133731 2134261 . - 0 Rv1884c rpfC Thought to promote the resuscitation and growth of dormant, nongrowing cell. Could also stimulates the growth of several other high G+C gram+ organisms, e.g. Mycobacterium avium, Mycobacterium bovis (BCG), Mycobacterium kansasii, Mycobacterium smegmatis. Probable resuscitation-promoting factor RpfC Rv1884c, (MTCY180.34), len: 176 aa. Probable rpfC, resuscitation promoting factor (see citation below), similar to Z96935|MLRPF_1 resusicitation-promoting factor from Micrococcus luteus (220 aa), FASTA score: opt: 287, E() : 3.3e-11, (40.0% identity in 120 aa overlap). Also similar to others from Mycobacterium tuberculosis: Rv2389c|MTCY253.32|RPFD probable resuscitation-promoting factor (154 aa), FASTA score: opt: 382, E(): 7.1e-17, (55.4% identity in 101 aa overlap); Rv0867c|RPFA (N-terminal part), Rv2450c|RPFE, and Rv1009|RPFB (C-terminal part). Predicted possible vaccine candidate (See Zvi et al., 2008). O07747 No cell wall and cell processes O07747 O07747 GO:0005576 O07747 ML2030 MMAR_2772 Mb1916c NC_000962.3 Mycobrowser_v4 CDS 2138661 2139017 . - 0 Rv1889c Rv1889c Function unknown Conserved hypothetical protein Rv1889c, (MTCY180.29), len: 118 aa. Conserved hypothetical protein. Part of large family of Mycobacterium tuberculosis proteins with conserved N-terminal domain of ~120 aa. Includes: Rv3399|Q50726|MTCY78.29C conserved hypothetical protein (348 aa), FASTA results: opt: 504, E(): 7.3e-29, (64.2% identity in 120 aa overlap); Rv0726c|P95074; Rv0731c; etc. Rv1888A possibly continuation of this CDS. O07743 No conserved hypotheticals O07743 O07743 GO:0008168 O07743 Mb1922c NC_000962.3 Mycobrowser_v4 CDS 2225841 2226101 . - 0 Rv1982A vapB36 Unknown Possible antitoxin VapB36 Rv1982A, len: 86 aa. Possible vapB36, antitoxin, part of toxin-antitoxin (TA) operon with Rv1982c, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. Rv0623, Rv2760c, Rv0608 P9WJ29 No virulence, detoxification, adaptation P9WJ29 NC_000962.3 Mycobrowser_v4 CDS 2316681 2317085 . - 0 Rv2061c Rv2061c Unknown Conserved protein Rv2061c, (MTV019.02c), len: 134 aa. Conserved protein. Similar to many. Contains IPR019965 F420-dependent enzyme, PPOX class, family Rv2061, domain. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O86340 No conserved hypotheticals O86340 O86340 GO:0010181 O86340 ML1446,ML1446c MMAR_3036 MSMEG_3863 Mb2087c NC_000962.3 Mycobrowser_v4 CDS 3501334 3501732 . + 0 Rv3135 PPE50 Function unknown PPE family protein PPE50 Rv3135, (MTCY03A2.23c), len: 132 aa. PPE50, Member of the Mycobacterium tuberculosis Ala-, Gly-rich PPE family, similar to P95190|Rv3136|MTCY03A2.22c (380 aa), FASTA scores: opt: 494, E(): 6.7e-25, (57.25% identity in 131 aa overlap) (next ORF downstream), MTY21C12_9, MTCY3C7_24, MTCI125_27, MTV049_12, MTV049_9, MTV049_11, MTCY274_24 etc. Q6MX07 No PE/PPE Q6MX07 Q6MX07 Q6MX07 Mb3159 NC_000962.3 Mycobrowser_v4 CDS 2136258 2137400 . + 0 Rv1887 Rv1887 Unknown Hypothetical protein Rv1887, (MTCY180.31), len: 380 aa. Hypothetical unknown protein; contains eukaryotic thiol (cysteine) proteases histidine active site at N-terminus (PS00639) and Pro-rich region near C-terminus. O07745 No conserved hypotheticals O07745 O07745 ML2027,ML2027c MMAR_2779 MSMEG_3531 Mb1919 NC_000962.3 Mycobrowser_v4 CDS 2142521 2143675 . + 0 Rv1895 Rv1895 Function unknown; probably involved in cellular metabolism Possible dehydrogenase Rv1895, (MTCY180.23c), len: 384 aa. Possible dehydrogenase, similar to various sorbitol and alcohol dehydrogenases, and to putative glutathione-dependent aldehyde dehydrogenase e.g DHSO_BACSU|Q06004 Sorbitol dehydrogenase from Streptomyces coelicolor (352 aa), FASTA results: opt: 506, E(): 7.2e-24, (30.6% identity in 350 aa overlap); and AL109962|SCJ1.28 putative zinc-containing dehydrogenase from Streptomyces coelicolor (356 aa), FASTA results: opt: 634, E(): 2.9e-30, (34.7% identity in 357 aa overlap). Also similar to other Mycobacterium tuberculosis dehydrogenases. Note that there is a substantial (134 bp) overlap at the C-terminus with the C-terminus of the downstream ORF, although both appear to be true coding regions. O07737 No intermediary metabolism and respiration O07737 O07737 GO:0016491,GO:0008270,GO:0055114 1.1.-.- O07737 MMAR_2790 Mb1928,Mb1929,Mb1930 NC_000962.3 Mycobrowser_v4 CDS 2143535 2144446 . - 0 Rv1896c Rv1896c Function unknown Conserved hypothetical protein Rv1896c, (MTCY180.22), len: 303 aa. Conserved hypothetical protein. Similar to several (14) hypothetical Mycobacterium tuberculosis proteins e.g. Rv0145|MTCI5.19 (317 aa), FASTA results: opt: 720, E(): 0, (41.6% identity in 308 aa overlap); Q10552|YZ21_MYCTU (325 aa), opt: 689, E(): 0, (40.5% identity in 304 aa overlap); Rv0726c, Rv0731c, Rv3399, etc. and to related proteins in other actinomycetes. Note that there is a substantial (134 bp) overlap at the C-terminus with the C-terminus of the downstream ORF, although both appear to be true coding regions. P9WFH7 No conserved hypotheticals O07736 P9WFH7 GO:0008168 2.1.1.- P9WFH7 ML2020 MMAR_2791 Mb1931c NC_000962.3 Mycobrowser_v4 CDS 2144451 2144882 . - 0 Rv1897c Rv1897c Function unknown Conserved hypothetical protein Rv1897c, (MTCY180.21), len: 143 aa. Conserved hypothetical protein. Some similarity to D63706|Q54235 ORF2 from Streptomyces griseus (149 aa), FASTA results: opt: 509, E(): 1.2e-28, (57.3% identity in 150 aa overlap); and Q45303 ORF1 protein from Corynebacterium glutamicum (144 aa), FASTA results: opt: 460, E(): 5.5e-23, (49.7% identity in 143 aa overlap). P9WNS9 No conserved hypotheticals P63995 P9WNS9 GO:0005737,GO:0016788,GO:0019478 3.1.-.- P9WNS9 ML2019 MMAR_2792 MSMEG_4291 Mb1932c NC_000962.3 Mycobrowser_v4 CDS 2144940 2145248 . + 0 Rv1898 Rv1898 Function unknown Conserved hypothetical protein Rv1898, (MTCY180.20c), len: 102 aa. Conserved hypothetical protein, some similarity to other hypothetical proteins e.g. Q58452 from methanococcus jannasch II (100 aa), FASTA results: opt: 152, E(): 9.1e-05, (31.5% identity in 92 aa overlap); and AE000771|AE000771_2 from Aquifex aeolicus (157 aa), FASTA results: opt: 246, E(): 3.2e-11, (39.0% identity in 100 aa overlap). P9WFQ1 No conserved hypotheticals P67119 P9WFQ1 P9WFQ1 ML2018,ML2018c MMAR_2793 MSMEG_0231 Mb1933 NC_000962.3 Mycobrowser_v4 CDS 2171061 2171525 . - 0 Rv1919c Rv1919c Function unknown Conserved protein Rv1919c, (MTV050.03c), len: 154 aa. Conserved protein, shows weak similarity to several major pollen antigens e.g. Z72431|BVGC25_1 major allergen bet V 1 from Betula verrucosa (160 aa), FASTA scores: opt: 133, E(): 0.012, (26.8% identity in 149 aa overlap). Also shows some similarity to Rv2574|MTCY227.27C Hypothetical protein from Mycobacterium tuberculosis (167 aa), (27.4% identity in 124 aa overlap). O53961 No conserved hypotheticals O53961 O53961 O53961 ML1983 Mb1954c NC_000962.3 Mycobrowser_v4 CDS 2147662 2148954 . + 0 Rv1901 cinA Unknown Probable CinA-like protein CinA Rv1901, (MTCY180.17c), len: 430 aa. Probable cinA-like protein, strong similarity to competence damage proteins CinA of Bacillus subtilis and S. pneumoniae. FASTA results: Q55760 hypothetical 44.7 kDa protein (416 aa) opt: 755, E(): 0, (36.0% identity in 433 aa overlap). P9WPE3 No virulence, detoxification, adaptation P63775 P9WPE3 GO:0006777 P9WPE3 ML2015,ML2015c MMAR_2796 MSMEG_3512 Mb1936 NC_000962.3 Mycobrowser_v4 CDS 2149006 2150274 . - 0 Rv1902c nanT Involved in transport of sialic acid across the membrane. Responsible for the translocation of the substrate across the membrane. Probable sialic acid-transport integral membrane protein NanT Rv1902c, (MTCY180.16), len: 422 aa. Probable nanT, sialic acid-transport integral membrane protein, possibly member of major facilitator superfamily (MFS), similar to others e.g. Q48076 sialic acid transporter (407 aa), FASTA results: opt: 443, E(): 5.4e-22, (26.7% identity in 389 aa overlap); etc. Some similarity to MTCI364.12|O05301 conserved hypothetical protein from Mycobacterium tuberculosis (425 aa), FASTA results: opt: 251, E(): 1.1e-09, (23.5% identity in 417 aa overlap). Contains sugar transport proteins signature 2 (PS00217). O07730 No cell wall and cell processes O07730 O07730 GO:0016021,GO:0055085,GO:0015144 ML2014 MMAR_2797 Mb1937c NC_000962.3 Mycobrowser_v4 CDS 2150364 2150768 . + 0 Rv1903 Rv1903 Unknown Probable conserved membrane protein Rv1903, (MTCY180.15c), len: 134 aa. Probable conserved membrane protein, similar to Q53868|YPT3_STRCO hypothetical 15.9 kDa protein from Streptomyces coelicolor (148 aa) opt: 323, E(): 1.3e-16, (42.9% identity in 126 aa overlap); and equivalent to AJ000521|MLCOSL672_3 from Mycobacterium leprae (139 aa), FASTA results: opt: 680, E(): 0, (80.6% identity in 129 aa overlap). O07729 No cell wall and cell processes O07729 O07729 ML2012,ML2012c MSMEG_5546 Mb1938 NC_000962.3 Mycobrowser_v4 CDS 2150954 2151385 . + 0 Rv1904 Rv1904 Function unknown Conserved hypothetical protein Rv1904, (MTCY180.14c), len: 143 aa. Conserved hypothetical protein, some similarity to other hypothetical Mycobacterium tuberculosis proteins e.g. Rv2638|MTCY441.08|P71937 (148 aa), FASTA results: opt: 456, E(): 2.7e-23, (52.8% identity in 125 aa overlap); Rv1365|Q11035 (128 aa), FASTA results: opt: 393, E(): 1.4e-19, (48.8% identity in 123 aa overlap); and Rv3687c. Also weak similarity to Q9WVX8|RSBV_STRCO anti-sigma B factor antagonist from Streptomyces coelicolor (113 aa). O07728 No conserved hypotheticals O07728 O07728 GO:0006355,GO:0045152 O07728 Mb1939 NC_000962.3 Mycobrowser_v4 CDS 2151433 2152395 . - 0 Rv1905c aao Wide specificity for D-amino acids. Also acts on glycine [catalytic activity: a D-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2] Probable D-amino acid oxidase Aao Rv1905c, (MTCY180.13), len: 320 aa. Probable aao, D-amino acid oxidase, similar to many. Equivalent to AJ000521|MLCOSL672.02|O33145 Mycobacterium leprae (320 aa), FASTA results: opt: 1541, E(): 0, (71.7% identity in 315 aa overlap); also similar to OXDD_BOVIN|P31228 d-aspartate oxidase from bos taurus (338 aa), FASTA results: opt: 461, E(): 1.1e-21, (31.8% identity in 321 aa overlap). P9WP27 No intermediary metabolism and respiration O07727 P9WP27 GO:0055114,GO:0003884 1.4.3.3 P9WP27 ML2011 MMAR_2801 Mb1940c NC_000962.3 Mycobrowser_v4 CDS 2152425 2152895 . - 0 Rv1906c Rv1906c Function unknown Conserved protein Rv1906c, (MTCY180.12), len: 156 aa. Conserved protein, possibly exported protein, equivalent to Mycobacterium leprae AJ000521|MLCOSL672.01 (153 aa), FASTA scores: opt: 637, E(): 2.6e-28, (63.2% identity in 155 aa overlap). Also similar to M. tuberculosis hypothetical exported protein, Rv1352. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). O07726 No conserved hypotheticals O07726 ML2010 MSMEG_3491 Mb1941c NC_000962.3 Mycobrowser_v4 CDS 2200726 2200941 . + 0 Rv1952 vapB14 Unknown Possible antitoxin VapB14 Rv1952, (MTCY09F9.12c), len: 71 aa. Possible vapB14, antitoxin, part of toxin-antitoxin (TA) operon with Rv1953 (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in M. tuberculosis e.g. Rv2601A. Some similarity to P55510|Y4JJ_RHISN putative plasmid stability protein (85 aa), FASTA scores: opt: 127, E(): 0.00096, (42.5% identity in 73 aa overlap). P95262 No virulence, detoxification, adaptation P95262 P95262 Mb1987 NC_000962.3 Mycobrowser_v4 CDS 2267119 2267724 . - 0 Rv2022c Rv2022c Unknown Conserved protein Rv2022c, (MTV018.09c), len: 201 aa. Conserved protein, similar to many. O53468 No conserved hypotheticals O53468 O53468 Mb2045c NC_000962.3 Mycobrowser_v4 CDS 2156149 2156592 . - 0 Rv1909c furA Acts as a global negative controlling element, employing FE(2+) as a cofactor to bind the operator of the repressed genes. Seems to regulate transcription of KATG|Rv1908c gene. Ferric uptake regulation protein FurA (fur) Rv1909c, (MTCY180.09), len: 147 aa. FurA, Ferric uptake regulation protein, similar to Q48835 legionella pneumophila 130B (wadsworth) ferric uptake regulation (136 aa), FASTA results: opt: 230, E(): 2.5e-09, (32.3% identity in 133 aa overlap). Also similar to Mycobacterium tuberculosis zur zinc uptake regulatory protein, Rv2359. Belongs to the fur family. Start changed since original submission (-3 aa). P9WN87 No regulatory proteins P0A582 P9WN87 GO:0046872,GO:0006355,GO:0006350,GO:0003700,GO:0005737 INH P9WN87 ML2008 MMAR_2915 MSMEG_3460,MSMEG_6383 Mb1944c NC_000962.3 Mycobrowser_v4 CDS 2159191 2159943 . + 0 Rv1913 Rv1913 Unknown Conserved hypothetical protein Rv1913, (MTCY180.05c), len: 250 aa. Conserved hypothetical protein, slight similarity to dehydrase and beta-lactamase precursors e.g. Q02057 dehydrase from Streptomyces coelicolor (297 aa), FASTA scores: opt: 184, E(): 4.3e-05, (31.6% identity in 215 aa overlap). O07720 No conserved hypotheticals O07720 GO:0016787 O07720 ML2001,ML2001c MMAR_2819 Mb1948 NC_000962.3 Mycobrowser_v4 CDS 2160463 2161566 . + 0 Rv1915 aceAa Involved in glyoxylate bypass, an alternative to the tricarboxylic acid cycle [catalytic activity: isocitrate = succinate + glyoxylate]. Probable isocitrate lyase AceAa [first part] (isocitrase) (isocitratase) (Icl) Rv1915, (MTCY180.03c), len: 367 aa. Probable aceAa, isocitrate lyase (see citations below). Highly similar to the N-terminus of ACEA_MYCLE isocitrate lyase from Mycobacterium leprae (606 aa), FASTA results: opt: 3314, E(): 0, (86.5% identity in 572 aa overlap). Contains PS00161 Isocitrate lyase signature. Although this ORF and the downstream ORF representing the C-terminal half of aceA could be joined by a frameshift, no error is apparent in the cosmid, or in a seqencing read from the genome of H37Rv. As the downstream ORF has a RBS and transcriptional start immediately following the stop of this ORF, it is possible that they are expressed as two separate modules. In Mycobacterium tuberculosis strain CDC1551, aceA exists as a single gene, MT1966: the corresponding protein has been purified experimentally and seems have an active isocitrate lyase activity (see Honer et al., 1999). For Mycobacterium tuberculosis strain H37Rv, immunoblot assay didn't detect AceAa or AceAb products (see Honer et al., 1999) but mRNA of AceAa|Rv1915 has been detected (see Betts et al., 2002); so AceAb|Rv1916 could be a pseudogene. Icl2 has 2-methyl-isocitrate lyase (MCL) activity in M. tuberculosis Erdman (See Munoz-Elias et al., 2006). O07718 No intermediary metabolism and respiration O07718 O07718 GO:0004451,GO:0019752 4.1.3.1 O07718 MSMEG_3706 Mb1950 NC_000962.3 Mycobrowser_v4 CDS 2510351 2510587 . - 0 Rv2237A Rv2237A Unknown Conserved protein Rv2237A, len: 78 aa. Conserved protein. I6XDU8 No conserved hypotheticals I6XDU8 I6XDU8 NC_000962.3 Mycobrowser_v4 CDS 2162932 2167311 . - 0 Rv1917c PPE34 Function unknown PPE family protein PPE34 Rv1917c, (MTV050.01c-MTCY180.01), len: 1459 aa. PPE34, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, MPTR subfamily (see citation below). Similar to MTCY28.16, MTCY13E10.17, MTCY63.10, MTV004.05, MTCY98.24, MTCY6G11.05, etc. C-terminus is identical to Q50471. Unknown Mycobacterium tuberculosis protein (693 aa), FASTA results: opt: 2635, E(): 0, (99.7% identity in 391 aa overlap). Start changed since original submission (+23 aa). Thougth to be surface exposed, cell-wall associated. Q79FI9 No PE/PPE Q79FI9 Q79FI9 Q79FI9 Mb1951c NC_000962.3 Mycobrowser_v4 CDS 2167649 2170612 . - 0 Rv1918c PPE35 Function unknown PPE family protein PPE35 Rv1918c, (MTV050.02c), len: 987 aa. PPE35, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins. Similar to MTCY28.16|Z95890 Mycobacterium tuberculosis cosmid (1053 aa), FASTA scores: opt: 3404, E(): 0, (65.6% identity in 1058 aa overlap). Also similar to MTV004.05, MTY13E10.17, MTV014.03, MTCY3C7.23, MTCY6G11.05, MTCY48.17, MTV004.03, MTCY31.07, MTCY4C12.36, MTCY180.01, etc. Q79FI8 No PE/PPE Q79FI8 Q79FI8 Q79FI8 Mb1952c,Mb1953c NC_000962.3 Mycobrowser_v4 CDS 2183866 2184957 . - 0 Rv1933c fadE18 Function unknown, but supposed involvement in lipid degradation. Probable acyl-CoA dehydrogenase FadE18 Rv1933c, (MTCY09F9.31), len: 363 aa. Probable fadE18, acyl-CoA dehydrogenase, similar to many e.g. CAB61609.1|AL133210 putative acyl-CoA dehydrogenase from Streptomyces coelicolor (362 aa); NP_421282.1|NC_002696 acyl-CoA dehydrogenase family protein from Caulobacter crescentus (344 aa); ACDS_RAT|P15651 short-chain specific acyl-CoA dehydrogenase from Rattus norvegicus (Rat) (412 aa), fasta scores: opt: 239, E(): 2.1e-08, (28.4% identity in 331 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. N-terminus of fadE22 (721 aa); fadE33 (318 aa); N-terminus of fadE34 (711 aa); etc. Could belong to the acyl-CoA dehydrogenases family. P95281 No lipid metabolism P95281 P95281 GO:0003995,GO:0055114,GO:0050660 1.3.99.- P95281 MMAR_2861 Mb1968c NC_000962.3 Mycobrowser_v4 CDS 2267749 2268108 . - 0 Rv2023c Rv2023c Unknown Hypothetical protein Rv2023c, (MTV018.10c), len: 119 aa. Hypothetical protein, alternative upstream start possible. O53469 No conserved hypotheticals O53469 O53469 Mb2046c NC_000962.3 Mycobrowser_v4 CDS 2282747 2283721 . - 0 Rv2037c Rv2037c Unknown Conserved transmembrane protein Rv2037c, (MTV018.24c), len: 324 aa. Conserved transmembrane protein, similar to many. Alternative nucleotide at position 2282787 (C->T; C312Y) has been observed. Contains IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase motif. L0TB61 No cell wall and cell processes O53481 L0TB61 GO:0006629,GO:0016021 L0TB61 ML1423,ML1423c MMAR_3010 Mb2063c NC_000962.3 Mycobrowser_v4 CDS 2172524 2173795 . - 0 Rv1921c lppF Unknown Probable conserved lipoprotein LppF Rv1921c, (MTCY09F9.43-MTV050.05c), len: 423 aa. Probable lppF, conserved lipoprotein, similar to G403173 lipoprotein precursor (fragment) from Rhodococcus erythropolis (225 aa), fasta scores: opt: 364, E(): 9.2e-19, (41.9% identity in 148 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O53963 No cell wall and cell processes O53963 O53963 MMAR_0949 Mb1956c NC_000962.3 Mycobrowser_v4 CDS 2175173 2176513 . + 0 Rv1923 lipD Function unknown; lipolytic enzyme probably involved in cellular metabolism Probable lipase LipD Rv1923, (MTCY09F9.41c), len: 446 aa. Probable lipD, hydrolase lipase, similar to esterases and beta-lactamases e.g. G151214 esterase, (389 aa), fasta scores: opt: 569, E(): 5.4e-29, (33.7% identity in 401 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins Rv1497, Rv2463, Rv3775, etc. P95290 No intermediary metabolism and respiration P95290 P95290 GO:0016787 3.1.-.- P95290 MMAR_2828 Mb1958 NC_000962.3 Mycobrowser_v4 CDS 2178957 2179436 . - 0 Rv1926c mpt63 Unknown Immunogenic protein Mpt63 (antigen Mpt63/MPB63) (16 kDa immunoprotective extracellular protein) Rv1926c, (MT1977, MTCY09F9.38), len: 159 aa. Mpt63 (alternate gene name: mpb63), immunogenic protein (see citations below), identical to MPT63|MPB63 from Mycobacterium bovis (159 aa). Exported protein containing a N-terminal signal sequence: see notes below about proteomics. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WIP1 No cell wall and cell processes 1LMI P0A5Q2 P9WIP1 GO:0005615 P9WIP1 MMAR_2839 MSMEG_5617 Mb1961c NC_000962.3 Mycobrowser_v4 CDS 2182460 2183239 . - 0 Rv1931c Rv1931c Involved in transcriptional mechanism Probable transcriptional regulatory protein Rv1931c, (MTCY09F9.33), len: 259 aa. Probable transcriptional regulatory protein. Similarity in C-terminal half to transcriptional activators e.g. Q43970 AraC-like protein (227 aa), FASTA scores: opt: 238, E(): 7.1e-07, (42.4% identity in 92 aa overlap). Similar to many probable transcription regulators in Streptomyces e.g. AL049587|SC5F2A.29 Streptomyces coelicolor (325 aa), FASTA scores: opt: 387, E(): 3.2e-16, (34.4% identity in 259 aa overlap). P95283 No regulatory proteins P95283 P95283 GO:0006355,GO:0043565,GO:0006350,GO:0003700,GO:0005622 P95283 MMAR_2855 MSMEG_1106 Mb1966c NC_000962.3 Mycobrowser_v4 CDS 2294531 2306986 . - 0 Rv2048c pks12 Involved in biosynthesis of mannosyl-beta-1-phosphomycoketide (MPM) Polyketide synthase Pks12 Rv2048c, (MTV018.35c), len: 4151 aa. Pks12, polyketide synthase similar to many. Contains 2x PS00012 Phosphopantetheine attachment site, 2x PS00606 Beta-ketoacyl synthases active site, and PS00343 Gram-positive cocci surface proteins 'anchoring' hexapeptide. Nucleotide position 2297976 in the genome sequence has been corrected, G:A resulting in S3004L. I6XD69 No lipid metabolism O53490 I6XD69 GO:0009058,GO:0048037,GO:0055114,GO:0016491,GO:0031177,GO:0016740,GO:0008270,GO:0000036 I6XD69 ML1437,ML1437c MMAR_3025 Mb2074c NC_000962.3 Mycobrowser_v4 CDS 2181262 2181906 . - 0 Rv1929c Rv1929c Function unknown Conserved hypothetical protein Rv1929c, MTCY09F9.35, len: 214 aa. Conserved hypothetical protein, similar to SC4G6.14|AL096884 hypothetical protein from Streptomyces coelicolor (211 aa), FASTA scores: opt: 416, E(): 2.4e-22, (39.8% identity in 206 aa overlap). P95285 No conserved hypotheticals P95285 P95285 MSMEG_0703 Mb1964c NC_000962.3 Mycobrowser_v4 CDS 2181918 2182442 . - 0 Rv1930c Rv1930c Function unknown Conserved hypothetical protein Rv1930c, MTCY09F9.34, len: 174 aa. Conserved hypothetical protein, similar to SC5F2A.30|AL049587 hypothetical protein from Streptomyces coelicolor (211 aa), FASTA scores: opt: 307, E(): 2.8e-13, (54.8% identity in 84 aa overlap). Some similarity to M. tuber culosis hypothetical protein Rv0052|MTCY21D4.15 (43% identity in 93 aa overlap). P95284 No conserved hypotheticals P95284 P95284 MMAR_2854 MSMEG_1107 Mb1965c NC_000962.3 Mycobrowser_v4 CDS 2186203 2187159 . - 0 Rv1935c echA13 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Possible enoyl-CoA hydratase EchA13 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv1935c, (MTCY09F9.29), len: 318 aa. Possible echA13, enoyl-CoA hydratase, similar to others and various enzymes e.g. CAC48381.1|Y16952 putative enoyl-CoA-isomerase from Amycolatopsis mediterranei (269 aa); AAK18173.1|AF290950_5|AF290950|FadB1x enoyl-CoA hydratase from Pseudomonas putida (257 aa); AAF78820.1|AF042490 4-chlorobenzoyl CoA dehalogenase from Arthrobacter sp. TM1 (276 aa); ECHM_RAT|P14604 enoyl-CoA hydratase mitochondrial precursor from Rattus norvegicus (Rat) (290 aa), FASTA scores: opt: 228, E(): 1.2e-08, (31.0% identity in 258 aa overlap); etc. P95279 No lipid metabolism P95279 P95279 GO:0016853,GO:0008152,GO:0004300 4.2.1.17 P95279 MMAR_2863 Mb1970c NC_000962.3 Mycobrowser_v4 CDS 2187384 2188493 . + 0 Rv1936 Rv1936 Function unknown; probably involved in cellular metabolism Possible monooxygenase Rv1936, (MTCY09F9.28c), len: 369 aa. Possible monooxygenase, similar to LXA2_PHOLU|P23146 alkanal monooxygenase alpha chain (362 aa), FASTA scores: opt: 196, E(): 6.3e-06, (22.3% identity in 373 aa overlap). Also similar to many other Mycobacterium tuberculosis hypothetical oxidoreductases and monooxygenases e.g. Rv0953c, Rv0791c, Rv0132c, etc. P95278 No intermediary metabolism and respiration P95278 P95278 GO:0055114,GO:0004497 1.-.-.- P95278 MMAR_2864 Mb1971 NC_000962.3 Mycobrowser_v4 CDS 2216592 2217167 . + 0 Rv1972 Rv1972 Unknown Probable conserved Mce associated membrane protein Rv1972, (MTV051.10), len: 191 aa. Probable conserved Mce-associated membrane protein. Probably part of mce3 operon. Similar to several Mycobacterium tuberculosis proteins e.g. Rv1363c|Z75555|MTCY02B10.27C (261 aa), FASTA scores: opt: 342, E(): 1.2e-15, (31.8% identity in 195 aa overlap); Rv1362c, Rv0177 (near Mce operon 1), etc. Has hydrophobic stretch at aa 20-40. O53973 No cell wall and cell processes O53973 O53973 MMAR_2888 MSMEG_0352,MSMEG_5208 NC_000962.3 Mycobrowser_v4 ncRNA 2299745 2299886 . + 0 MTB000057 ASpks Unknown Putative small regulatory RNA ASpks, putative small regulatory RNA (See Arnvig and Young, 2009). Alternate 5'-ends at positions 2299785 and 2299796. Alternate 3'-end at position 2299873. This sequence is repeated in pks12|Rv2048c at position 2305814-2305955. No stable RNAs NC_000962.3 Mycobrowser_v4 CDS 2191027 2192097 . + 0 Rv1938 ephB This enzyme acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potential toxic epoxides. Also determines steady-state levels of physiological mediators. Probable epoxide hydrolase EphB (epoxide hydratase) Rv1938, (MTCY09F9.26c), len: 356 aa. Probable ephB, epoxide hydrolase (see citation below), similar to many e.g. G1109600 ATSEH (321 aa), FASTA scores: opt: 442, E(): 1.2e-21 (33.1% identity in 356 aa overlap); etc. Also similar to many other M. tuberculosis hypothetical epoxide hydrolases e.g. Rv3617, Rv3670, Rv0134, etc. I6YC03 No virulence, detoxification, adaptation 2ZJF,2E3J P95276 I6YC03 GO:0016787 3.3.2.3 I6YC03 MMAR_2866 Mb1973 NC_000962.3 Mycobrowser_v4 CDS 2192606 2193667 . + 0 Rv1940 ribA1 Involved in riboflavin biosynthesis [catalytic activity: GTP + 3 H(2)O = formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate] Probable riboflavin biosynthesis protein RibA1 (GTP cyclohydrolase II) Rv1940, (MTCY09F9.24c), len: 353 aa. Probable ribA1, Riboflavin biosynthesis protein, similar to GCH2_BACSU|P17620 gtp cyclohydrolase II (398 aa), FASTA scores: opt: 682, E(): 0, (37.7% identity in 363 aa overlap), also similar to Rv1415|MTCY21B4.33|ribA2 (428 aa) (45.4% identity in 368 aa overlap). Note that previously known as ribA. L7N669 No intermediary metabolism and respiration Q7D7R0 L7N669 GO:0003935,GO:0009231,GO:0008686 3.5.4.25 L7N669 MMAR_2868 Mb1975 NC_000962.3 Mycobrowser_v4 CDS 2193664 2194434 . + 0 Rv1941 Rv1941 Function unknown; probably involved in cellular metabolism Probable short-chain type dehydrogenase/reductase Rv1941, (MTCY09F9.23c), len: 256 aa. Probable short-chain dehydrogenase/reductase, similar to various dehydrogenases/reductases, generally belonging to SDR family, e.g. NP_299015.1|NC_002488 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase from Xylella fastidiosa (255 aa); NP_250340.1|NC_002516 probable short-chain dehydrogenase from Pseudomonas aeruginosa (253 aa); NP_106890.1|NC_002678 probable short-chain type dehydrogenase/reductase from Mesorhizobium loti (374 aa) (has its N-terminus longter); P50197|LINC_PSEPA 2,5-dichloro-2,5-cyclohexadiene-1,4-dehydrogenase from Pseudomonas paucimobilis (Sphingomonas paucimobilis) (250 aa), FASTA scores: opt: 529, E(): 5.7e-25, (40.6% identity in 251 aa overlap); etc. Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. I6XZC4 No intermediary metabolism and respiration 3GVC P95273 I6XZC4 GO:0055114,GO:0016491 1.-.-.- I6XZC4 Mb1976 NC_000962.3 Mycobrowser_v4 CDS 2195989 2197353 . + 0 Rv1945 Rv1945 Unknown Conserved hypothetical protein Rv1945, (MTCY09F9.19c), len: 454 aa. Member of Mycobacterium tuberculosis REP13E12 repeat family. Similar to several others, best with Rv1148c|Z95584|MTCI65.15 (482 aa), FASTA score: opt: 2954, E(): 0, (97.1% identity in 454 aa overlap). Contains possible helix-turn-helix motif at aa 74-95 (+2.90 SD). P9WLQ5 No insertion seqs and phages P95269 P9WLQ5 P9WLQ5 MSMEG_2663 Mb1980 NC_000962.3 Mycobrowser_v4 CDS 2555941 2556135 . + 0 Rv2283 Rv2283 Unknown Hypothetical protein Rv2283, (MTCY339.27c), len: 64 aa. Unknown protein; questionable ORF. P9WLE9 No conserved hypotheticals P9WLE9 Mb2304 NC_000962.3 Mycobrowser_v4 CDS 2203974 2204225 . - 0 Rv1960c parD1 Function unknown Possible antitoxin ParD1 Rv1960c, (MTCY09F9.04), len: 83 aa. Possible parD1, antitoxin, part of toxin-antitoxin (TA) operon with Rv1959c (See Pandey and Gerdes, 2005), similar to O85269|AF102990|AF102990_51 hypothetical protein of Yersinia enterocolitica (80 aa), FASTA scores: opt: 149, E(): 0.00037, (42.1% identity in 57 aa overlap). P9WIJ7 No virulence, detoxification, adaptation P9WIJ7 GO:0045449 P9WIJ7 Mb1995c NC_000962.3 Mycobrowser_v4 CDS 2197508 2197960 . - 0 Rv1946c lppG Unknown Possible lipoprotein Rv1946c, (MTCY09F9.18), len: 150 aa. Possible lppG, conserved lipoprotein, showing some similarity to Rv1943c|MTCY09F9.21 conserved hypothetical protein from Mycobacterium tuberculosis (125 aa), FASTA score: (71.4% identity in 28 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P95268 No cell wall and cell processes P95268 Mb1981c NC_000962.3 Mycobrowser_v4 CDS 2198024 2198425 . + 0 Rv1947 Rv1947 Unknown Hypothetical protein Rv1947, (MTCY09F9.17c), len: 133 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P95267 No conserved hypotheticals P95267 Mb1982 NC_000962.3 Mycobrowser_v4 CDS 2198714 2199064 . - 0 Rv1948c Rv1948c Unknown Hypothetical protein Rv1948c, (MTCY09F9.16), len: 116 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P95266 No conserved hypotheticals P95266 Mb1983c NC_000962.3 Mycobrowser_v4 CDS 2199075 2200034 . - 0 Rv1949c Rv1949c Function unknown Conserved hypothetical protein Rv1949c, (MTCY09F9.15), len: 319 aa. Conserved hypothetical protein, partial ORF. Rv1949c and Rv1950c|MTCY09F9.14 are similar but frameshifted with respect to Rv2077c|MTCY49.16C|Q10685 hypothetical 33.3 kd protein (323 aa), FASTA scores: opt: 459, E(): 2.8e-16, (54.8% identity in 157 aa overlap). Cosmid sequence appears to be correct, genomic sequence is also frameshifted in Mycobacterium bovis strain AF2122/97. Similar to Mycobacterium tuberculosis hypothetical proteins: Rv2542, Rv2077c, Rv2797c, Rv0963c, etc. L0T9Q6 No conserved hypotheticals L0T9Q6 L0T9Q6 MMAR_0482 Mb1984c NC_000962.3 Mycobrowser_v4 CDS 2199998 2200189 . - 0 Rv1950c Rv1950c Function unknown Conserved hypothetical protein Rv1950c, (MTCY09F9.14), len: 63 aa. Conserved hypothetical protein, partial ORF. Highly similar to N-terminus of Rv2077c|MTCY49.16C|Q10685 hypothetical 33.3 kDa protein (323 aa), FASTA scores: opt: 280, E(): 1.2 e-16, (71.7% identity in 53 aa overlap) but homology continues in different frame ie MTCY09F9.15, cosmid sequence appears to be correct, genomic sequence is also frameshifted in Mycobacterium bovis strain AF2122/97. P95264 No conserved hypotheticals P95264 Mb1985c NC_000962.3 Mycobrowser_v4 CDS 2201719 2202096 . + 0 Rv1955 higB Unknown Possible toxin HigB Rv1955, (MTCY09F9.09c), len: 125 aa. Possible higB, toxin, part of toxin-antitoxin (TA) operon with Rv1956 (See Pandey and Gerdes, 2005; Gupta, 2009). Start overlaps another ORF, Rv1954c (MTCY09F9.10). Start changed since first submission (-45 aa). Predicted to be an outer membrane protein (See Song et al., 2008). Upon expression in E. coli has been shown to function as an antitoxin against Rv1956 (PubMed: 19016878); It is not clear if these conflicting results are due to expression in a heterologous system; In various publications, both gene names higA and higB have been assigned to both Rv1955 and Rv1956; we have chosen to call Rv1955 higB after consulting the authors. P9WJA5 No virulence, detoxification, adaptation P95259 P9WJA5 P9WJA5 Mb1990 NC_000962.3 Mycobrowser_v4 CDS 2246832 2247584 . + 0 Rv2001 Rv2001 Unknown Conserved hypothetical protein Rv2001, (MTCY39.17c), len: 250 aa. Conserved hypothetical protein. Similar to Mycobacterium tuberculosis Rv0466. P9WLN5 No conserved hypotheticals Q10856 P9WLN5 P9WLN5 MMAR_2977 Mb2024 NC_000962.3 Mycobrowser_v4 CDS 2203018 2203632 . - 0 Rv1958c Rv1958c Unknown Hypothetical protein Rv1958c, (MTCY09F9.06), len: 204 aa. Hypothetical unknown protein, questionable ORF P95256 No conserved hypotheticals P95256 P95256 Mb1993c NC_000962.3 Mycobrowser_v4 CDS 2203681 2203977 . - 0 Rv1959c parE1 Function unknown Possible toxin ParE1 Rv1959c, (MTCY09F9.05), len: 98 aa. Possible parE1, toxin, part of toxin-antitoxin (TA) operon with Rv1960c (See Pandey and Gerdes, 2005), similar to other hypothetical plasmid proteins e.g. AL117189|YPCD1.08 from Yersinia pestis (99 aa), FASTA scores: opt: 162, E(): 7.3e-05, (33.0% identity in 91 aa overlap); also some similarity to E145339 hypothetical protein (103 aa), FASTA scores: opt: 142, E(): 0.0003, (33.0% identity in 91 aa overlap). P9WHG7 No virulence, detoxification, adaptation P95255 P9WHG7 P9WHG7 Mb1994c NC_000962.3 Mycobrowser_v4 -35_signal 2207496 2207501 . + 0 Rv1964 yrbE3A Unknown Conserved hypothetical integral membrane protein YrbE3A Rv1964, (MTV051.02), len: 265 aa. YrbE3A, hypothetical unknown integral membrane protein, part of mce3 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07412|Rv0167|MTCI28.07|yrbE1A (265 aa), O07791|Rv0587|MTCY19H5.35|yrbE2A (265 aa), Rv3501c|MTV023.08c|yrbE4A (254 aa), etc. Also highly similar to conserved hypothetical integral membrane proteins of yrbEA type, e.g. AAD24544.1|AF116213|YrbE1A from Mycobacterium leprae (112 aa); P45392|YRBE_ECOLI from Escherichia coli (260 aa), FASTA scores: opt: 893, E(): 0, (51.4% identity in 253 aa overlap); etc. The transcription of this CDS seems negatively regulated by the product of Rv1963c|mce3R (see Santangelo et al., 2002). O53965 No virulence, detoxification, adaptation O53965 O53965 O53965 MMAR_2880 Mb1999 NC_000962.3 Mycobrowser_v4 -10_signal 2207518 2207523 . + 0 Rv1964 yrbE3A Unknown Conserved hypothetical integral membrane protein YrbE3A Rv1964, (MTV051.02), len: 265 aa. YrbE3A, hypothetical unknown integral membrane protein, part of mce3 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07412|Rv0167|MTCI28.07|yrbE1A (265 aa), O07791|Rv0587|MTCY19H5.35|yrbE2A (265 aa), Rv3501c|MTV023.08c|yrbE4A (254 aa), etc. Also highly similar to conserved hypothetical integral membrane proteins of yrbEA type, e.g. AAD24544.1|AF116213|YrbE1A from Mycobacterium leprae (112 aa); P45392|YRBE_ECOLI from Escherichia coli (260 aa), FASTA scores: opt: 893, E(): 0, (51.4% identity in 253 aa overlap); etc. The transcription of this CDS seems negatively regulated by the product of Rv1963c|mce3R (see Santangelo et al., 2002). O53965 No virulence, detoxification, adaptation O53965 O53965 O53965 MMAR_2880 Mb1999 NC_000962.3 Mycobrowser_v4 CDS 2207700 2208497 . + 0 Rv1964 yrbE3A Unknown Conserved hypothetical integral membrane protein YrbE3A Rv1964, (MTV051.02), len: 265 aa. YrbE3A, hypothetical unknown integral membrane protein, part of mce3 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07412|Rv0167|MTCI28.07|yrbE1A (265 aa), O07791|Rv0587|MTCY19H5.35|yrbE2A (265 aa), Rv3501c|MTV023.08c|yrbE4A (254 aa), etc. Also highly similar to conserved hypothetical integral membrane proteins of yrbEA type, e.g. AAD24544.1|AF116213|YrbE1A from Mycobacterium leprae (112 aa); P45392|YRBE_ECOLI from Escherichia coli (260 aa), FASTA scores: opt: 893, E(): 0, (51.4% identity in 253 aa overlap); etc. The transcription of this CDS seems negatively regulated by the product of Rv1963c|mce3R (see Santangelo et al., 2002). O53965 No virulence, detoxification, adaptation O53965 O53965 O53965 MMAR_2880 Mb1999 NC_000962.3 Mycobrowser_v4 CDS 2217164 2217646 . + 0 Rv1973 Rv1973 Unknown Possible conserved Mce associated membrane protein Rv1973, (MTV051.11), len: 160 aa. Possible conserved Mce-associated membrane protein. Probably part of mce3 operon. Similar to several other proteins from Mycobacterium tuberculosis e.g. Rv1362c|Z75555|MTCY02B10.26C (220 aa), FASTA scores: opt: 378, E(): 2.8e-19, (50.0% identity in 128 aa overlap); Rv1363c; Rv0177 (near Mce operon 1); etc. Contains possible N-terminal signal sequence or membrane anchor. Predicted to be an outer membrane protein (See Song et al., 2008). P9WJ77 No cell wall and cell processes P9WJ77 P9WJ77 MMAR_2889 MSMEG_0353 NC_000962.3 Mycobrowser_v4 CDS 2238141 2238908 . + 0 Rv1995 Rv1995 Unknown Unknown protein Rv1995, (MTCY39.24c), len: 255 aa. Unknown protein. P9WLP3 No conserved hypotheticals P64915 P9WLP3 P9WLP3 MMAR_2968 Mb2018 NC_000962.3 Mycobrowser_v4 CDS 2591848 2592726 . - 0 Rv2319c Rv2319c Unknown Universal stress protein family protein Rv2319c, (MTCY3G12.15), len: 292 aa. Universal stress protein family protein. P9WLB5 No virulence, detoxification, adaptation P64995 P9WLB5 GO:0006950 P9WLB5 ML1771,ML1771c MMAR_3620 MSMEG_1411 Mb2346c NC_000962.3 Mycobrowser_v4 CDS 2210601 2211629 . + 0 Rv1967 mce3B Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce3B Rv1967, (MTV051.05), len: 342 aa. Mce3B; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07414|Rv0170|MTCI28.10|mce1B (346 aa); O07788|Rv0590|MTCY19H5.32c|mce2B (275 aa); etc. Also similar to others e.g. NP_302657.1|NC_002677 putative secreted protein from Mycobacterium leprae (346 aa); CAC12797.1|AL445327 putative secreted protein from Streptomyces coelicolor (354 aa); etc. Contains a possible N-terminal signal sequence or membrane anchor. The transcription of this CDS seems negatively regulated by the product of Rv1963c|mce3R (see Santangelo et al., 2002). Predicted to be an outer membrane protein (See Song et al., 2008). O53968 No virulence, detoxification, adaptation O53968 O53968 O53968 MMAR_2883 MSMEG_0347 NC_000962.3 Mycobrowser_v4 CDS 2212855 2214126 . + 0 Rv1969 mce3D Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce3D Rv1969, (MTV051.07), len: 423 aa. Mce3D; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07416|Rv0172|MTCI28.12|mce1D (530 aa); O07786|Rv0592|MTCY19H5.30c|mce2D (508 aa); etc. Also highly similar to others e.g. NP_302659.1|NC_002677 putative secreted protein from Mycobacterium leprae (531 aa); CAC12795.1|AL445327 putative secreted protein from Streptomyces coelicolor (337 aa); etc. Contains a possible N-terminal signal sequence or membrane anchor. The transcription of this CDS seems negatively regulated by the product of Rv1963c|mce3R (see Santangelo et al., 2002). Predicted to be an outer membrane protein (See Song et al., 2008). O53970 No virulence, detoxification, adaptation O53970 O53970 O53970 MMAR_2885 MSMEG_0349 NC_000962.3 Mycobrowser_v4 CDS 2214123 2215256 . + 0 Rv1970 lprM Unknown, but thought to be involved in host cell invasion. Possible Mce-family lipoprotein LprM (Mce-family lipoprotein Mce3E) Rv1970, (MTV051.08), len: 377 aa. Possible lprM (alternate gene name: mce3E), lipoprotein which belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07417|LPRK|Rv0173|MTCI28.13|mce1E (390 aa); O07785|LPRL|Rv0593|MTCY19H5.29|mce2E (402 aa); etc. Also highly similar to others e.g. NP_302660.1|NC_002677 putative lipoprotein from Mycobacterium leprae (392 aa); CAC12794.1|AL445327 putative secreted protein from Streptomyces coelicolor (413 aa); etc. Contains possible N-terminal signal sequence or membrane anchor and PS00013 Prokaryotic membrane lipoprotein lipid attachment site. The transcription of this CDS seems negatively regulated by the product of Rv1963c|mce3R (see Santangelo et al., 2002). O53971 No cell wall and cell processes O53971 O53971 O53971 MMAR_2886 MSMEG_0350 NC_000962.3 Mycobrowser_v4 CDS 2215257 2216570 . + 0 Rv1971 mce3F Unknown, but thought involved in host cell invasion. Mce-family protein Mce3F Rv1971, (MTV051.09), len: 437 aa. Mce3F; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), similar to Mycobacterium tuberculosis proteins O07418|Rv0174|MTCI28.14|mce1F (515 aa), O07784|Rv0594|MTCY19H5.28c|mce2F (516 aa); etc. Also highly similar to others e.g. NP_302661.1|NC_002677 putative secreted protein from Mycobacterium leprae (516 aa); CAC12793.1|AL445327 putative secreted protein from Streptomyces coelicolor (433 aa); etc. Contains a possible N-terminal signal sequence or membrane anchor. The transcription of this CDS seems negatively regulated by the product of Rv1963c|mce3R (see Santangelo et al., 2002). Predicted to be an outer membrane protein (See Song et al., 2008). O53972 No virulence, detoxification, adaptation O53972 O53972 O53972 MMAR_2887 MSMEG_0351 NC_000962.3 Mycobrowser_v4 CDS 2227908 2228561 . - 0 Rv1984c cfp21 Hydrolyzes cutin. Shown to have esterase and lipase activity. Probable cutinase precursor CFP21 Rv1984c, (MTCY39.35), len: 217 aa. Cfp21, probable cutinase precursor with N-terminal signal sequence, similar to P41744|CUTI_ALTBR cutinase precursor from Alternaria brassicicola (209 aa), FASTA scores: opt: 283, E(): 2.2e-11, (32.6% identity in 193 aa overlap). Also similar to Mycobacterium tuberculosis proteins e.g. Rv3452, Rv3451, Rv2301, Rv1758, Rv3724. Belongs to the cutinase family. P9WP43 No cell wall and cell processes P63879 P9WP43 GO:0005576,GO:0008152,GO:0050525 3.1.1.74 P9WP43 MMAR_2934 Mb2006c NC_000962.3 Mycobrowser_v4 CDS 2220908 2221756 . + 0 Rv1978 Rv1978 Function unknown Conserved protein Rv1978, (MTV051.16), len: 282 aa. Conserved protein, similar to several hypothetical proteins and methyltransferases e.g. X86780|SHGCPIR.15 methyltransferase from S. hygroscopicus (211 aa), FASTA scores: opt: 151, E(): 0.0072, (30.6% identity in 121 aa overlap). O53979 No conserved hypotheticals O53979 O53979 O53979 MSMEG_6231 Mb2000 NC_000962.3 Mycobrowser_v4 CDS 2221719 2223164 . - 0 Rv1979c Rv1979c Unknown; possibly involved in transport of amino acid across the membrane. Possible conserved permease Rv1979c, (MTCY39.40-MTV051.17c), len: 481 aa. Possible permease, APC family possibly involved in transport of amino acid, showing some similarity to other permeases. Also similar to MTCY39.19 from Mycobacterium tuberculosis (28.2% identity in 277 aa overlap). Contains PS00599 Aminotransferases class-II pyridoxal-phosphate attachment site. Nucleotide position 2221796 in the genome sequence has been corrected, C:T resulting in V457I. P9WQM5 No cell wall and cell processes Q10875 P9WQM5 GO:0006865,GO:0016021,GO:0005886,GO:0055085,GO:0015171 P9WQM5 ML1974 MSMEG_1508,MSMEG_6735 Mb2001c NC_000962.3 Mycobrowser_v4 CDS 2223343 2224029 . - 0 Rv1980c mpt64 Unknown Immunogenic protein Mpt64 (antigen Mpt64/MPB64) Rv1980c, (MT2032, MTCY39.39), len: 228 aa. Mpt64 (alternate gene name: mpb64), immunogenic protein (alternate gene name: mpb64) (see citations below), identical to MPT64|MPB64 from Mycobacterium bovis (228 aa). Similar to Rv3036c|MTV012.51c from Mycobacterium tuberculosis. Exported protein containing a N-terminal signal sequence: see notes below about proteomics. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WIN9 No cell wall and cell processes 2HHI P0A5Q4 P9WIN9 GO:0005576 P9WIN9 MMAR_5409 Mb2002c NC_000962.3 Mycobrowser_v4 CDS 2247660 2248442 . + 0 Rv2002 fabG3 Not really known; thought to be involved in lipid biosynthesis [catalytic activity: androstan-3-alpha,17-beta-DIOL + NAD+ = 17-beta-hydroxyandrostan-3-one + NADH]. Possible 20-beta-hydroxysteroid dehydrogenase FabG3 (cortisone reductase) ((R)-20-hydroxysteroid dehydrogenase) Rv2002, (MTCY39.16c), len: 260 aa. FabG3, 20-beta-hydroxysteroid dehydrogenase. Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. P9WGT1 No lipid metabolism 1NFF,1NFQ,1NFR P69167 P9WGT1 GO:0055114,GO:0008202,GO:0047044 1.1.1.53 P9WGT1 MMAR_2981 Mb2025 NC_000962.3 Mycobrowser_v4 CDS 2252002 2255985 . + 0 Rv2006 otsB1 Involved in trehalose biosynthesis (protective effect). Mycobacteria can produce trehalose from glucose 6-phosphate and UDP-glucose (the OtsA-OtsB pathway) from glycogen-like alpha(1-->4)-linked glucose polymers (the TreY-TreZ pathway) and from maltose (the TreS pathway) [catalytic activity: trehalose 6-phosphate + H(2)O = trehalose + orthophosphate]. Probable trehalose-6-phosphate phosphatase OtsB1 (trehalose-phosphatase) (TPP) Rv2006, (MTCY39.11c), len: 1327 aa. OtsB1, trehalose-6-phosphate phosphatase (see citations below). Belongs to Glycosyl hydrolases family 65. Note that previously known as otsB. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WN15 No virulence, detoxification, adaptation Q10850 P9WN15 GO:0016798,GO:0005992,GO:0030246 3.2.1.- P9WN15 MMAR_2257 MSMEG_3954 Mb2029 NC_000962.3 Mycobrowser_v4 CDS 2945847 2946287 . - 0 Rv2617c Rv2617c Unknown Probable transmembrane protein Rv2617c, (MTCY01A10.17), len: 146 aa. Probable transmembrane protein, showing some similarity to hypothetical or membrane proteins e.g. CAC47207|SMC00744 putative transport protein transmembrane from Rhizobium meliloti (Sinorhizobium meliloti) (399 aa), FASTA scores: opt: 108, E(): 5.5, (29.15% identity in 144 aa overlap). I6XER9 No cell wall and cell processes O06197 I6XER9 GO:0016021 ML0460,ML0460c Mb2650c NC_000962.3 Mycobrowser_v4 CDS 2228991 2229902 . - 0 Rv1985c Rv1985c Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably LysR-family) Rv1985c, (MTCY39.34), len: 303 aa. Probable transcriptional regulatory protein, LysR family member. Similar to many regulatory proteins, especially ICIA_ECOLI|P24194 chromosome initiation inhibitor from Escherichia coli (297 aa), FASTA scores: opt: 520, E(): 1.1e-28, (35.8% identity in 285 aa overlap); and P94632|LYSG_CORGL lysine export regulator protein (290 aa), FASTA scores: opt: 705, E(): 0, (42.7% identity in 288 aa overlap); etc. Contains PS00044 Bacterial regulatory proteins, lysR family signature. Also contains helix-turn-helix motif at aa 22-43,(+5.52 SD). Belongs to the LysR family of transcriptional regulators. P9WMF5 No regulatory proteins 3ISP P67665 P9WMF5 GO:0006350,GO:0003700,GO:0006355 P9WMF5 ML2286 MSMEG_0548 Mb2007c NC_000962.3 Mycobrowser_v4 CDS 2230011 2230610 . + 0 Rv1986 Rv1986 Unknown; possibly involved in transport of lysine across the membrane. Probable conserved integral membrane protein Rv1986, (MTCY39.33c), len: 199 aa. Probable conserved integral membrane protein, LysE family possibly involved in transport of Lysine, similar to P11667|YGGA_ECOLI hypothetical 23.2 kDa protein in sbm-fba intergenic region (211 aa), FASTA scores: opt: 379, E(): 1.5e-19, (37.3% identity in 185 aa overlap); and Q11154|Rv0488 hypothetical 20.9 kDa protein from M. tuberculosis (201 aa), FASTA scores: opt: 784, E(): 0, (63.4% identity in 186 aa overlap). Belongs to the LYSE/YGGA family. P9WK31 No cell wall and cell processes P64903 P9WK31 GO:0016021,GO:0005886,GO:0006865 ML2285,ML2285c MSMEG_0467 Mb2008 NC_000962.3 Mycobrowser_v4 CDS 2231026 2231454 . + 0 Rv1987 Rv1987 Hydrolysis of chitin Possible chitinase Rv1987, (MTCY39.32c), len: 142 aa. Possible chitinase, similar to several e.g. P36909|CHIT_STRLI chitinase c precursor (619 aa) FASTA scores, opt: 324, E(): 1.2e-14, (39.5% identity in 129 aa overlap). P9WLQ1 No cell wall and cell processes P64905 P9WLQ1 GO:0004553,GO:0030247,GO:0005975 P9WLQ1 MMAR_2951 Mb2009 NC_000962.3 Mycobrowser_v4 CDS 2231680 2232219 . + 0 Rv1988 erm(37) Thought to cause methylation of 23S rRNA Probable 23S rRNA methyltransferase Erm(37) Rv1988, (MTCY39.31c), len: 179 aa. Probable erm(37), 23S rRNA methyltransferase, similar to ERME_SACER|P07287 rrna adenine n-6-methyltransferase (370 aa), FASTA scores: opt: 259, E(): 2e-11, (35.1% identity in 171 aa overlap); contains PS00092 N-6 Adenine-specific DNA methylases signature. Also similar to Mycobacterium tuberculosis Rv1010 ksgA 16S rRNA dimethyltransferase. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q10838 No intermediary metabolism and respiration Q10838 Q10838 GO:0000154,GO:0000179 2.1.1.- Q10838 Mb2010 NC_000962.3 Mycobrowser_v4 CDS 2232739 2233299 . - 0 Rv1989c Rv1989c Unknown Hypothetical protein Rv1989c, (MTCY39.30), len: 186 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLP9 No conserved hypotheticals P64907 P9WLP9 GO:0005886,GO:0016021 Mb2011c NC_000962.3 Mycobrowser_v4 CDS 2233296 2233637 . - 0 Rv1990c Rv1990c Involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv1990c, (MTCY39.29), len: 113 aa. Probable transcriptional regulatory protein, similar to Mycobacterium tuberculosis Rv3188|AL021646|MTV014.32 (115 aa), FASTA scores: opt: 184, E(): 8.2e-07, (28.4% identity in 109 aa overlap). Contains probable helix-turn-helix motif at aa 20-44 (+4.22 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLP7 No regulatory proteins P9WLP7 Mb2012c NC_000962.3 Mycobrowser_v4 CDS 2233881 2234216 . - 0 Rv1990A Rv1990A Function unknown; probably involved in cellular metabolism. Possible dehydrogenase (fragment) Rv1990A, len: 111 aa. Possible dehydrogenase (fragment), similar to N-terminal part of several dehydrogenases and hypothetical proteins, e.g. Rv2750|MTV002.15|AL008967 from Mycobacterium tuberculosis (272 aa), FASTA scores: opt: 151, E(): 0.0045, (47.45% identity in 78 aa overlap), but lacks C-terminal part. Maybe a pseudogene. Also similar to U17129|RSU17129_7 putative short-chain alcohol dehydrogenase from Rhodococcus erythropolis (275 aa), FASTA scores: opt: 142, E(): 0.018, (54.15% identity in 48 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L7N6A3 No intermediary metabolism and respiration L7N6A3 GO:0055114,GO:0016491 1.-.-.- L7N6A3 Mb2013c NC_000962.3 Mycobrowser_v4 CDS 2248563 2249420 . - 0 Rv2003c Rv2003c Unknown Conserved hypothetical protein Rv2003c, (MTCY39.14), len: 285 aa. Conserved hypothetical protein. P9WJZ5 No conserved hypotheticals P64919 P9WJZ5 GO:0008168,GO:0008152 P9WJZ5 Mb2026c NC_000962.3 Mycobrowser_v4 CDS 2321057 2321467 . + 0 Rv2063A mazF7 Unknown Possible toxin MazF7 Rv2063A, len: 136 aa. Possible mazF7 toxin, part of toxin-antitoxin (TA) operon with Rv2063 (See Pandey and Gerdes, 2005). P0CL62 No virulence, detoxification, adaptation P0CL62 P0CL62 NC_000962.3 Mycobrowser_v4 CDS 2234305 2234649 . - 0 Rv1991c mazF6 Sequence-specific mRNA cleavage Toxin MazF6 Rv1991c, (MTCY39.28), len: 114 aa. MazF6, toxin, part of toxin-antitoxin (TA) operon with Rv1991A. Some similarity to P13976|PEMK_ECOLI pemk protein (133 aa), FASTA scores: opt: 113, E(): 0.043, (29.2% identity in 113 aa overlap); and P96622|YDCE protein from Bacillus subtilis (116 aa), FASTA scores: opt: 227, E(): 6.9e-09, (37.4% identity in 115 aa overlap). Also similar to Mycobacterium tuberculosis Rv2801c, and Rv0659c. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WII3 No virulence, detoxification, adaptation P64911 P9WII3 GO:0003677 P9WII3 Mb2014c NC_000962.3 Mycobrowser_v4 CDS 2234643 2234891 . - 0 Rv1991A mazE6 Unknown Antitoxin MazE6 Rv1991A, len: 82 aa. MazE6, antitoxin, part of toxin-antitoxin (TA) operon with Rv1991c. Similar to ChpI of L. interrogans, FASTA scores: opt: 134, E(): 0.024, 29.762% identity (65.476% similar) in 84 aa overlap. Note that Pandey and Gerdes, 2005 predicts a different N-terminus, adding 10 amino acids. P9WJ87 No virulence, detoxification, adaptation P9WJ87 NC_000962.3 Mycobrowser_v4 CDS 2237303 2237575 . - 0 Rv1993c Rv1993c Function unknown Conserved protein Rv1993c, (MTCY39.26), len: 90 aa. Conserved protein, very similar to Rv3269|Z92771|MTCY71.09 hypothetical protein from Mycobacterium tuberculosis (93 aa), FASTA results: opt: 309, E(): 3.2e-16, (63.3% identity in 79 aa overlap). Also similar to Rv0968 (98 aa) (51.1% identity in 94 aa overlap). P9WLP5 No conserved hypotheticals P64913 P9WLP5 MMAR_1430,MMAR_2146 MSMEG_6059 Mb2016c NC_000962.3 Mycobrowser_v4 CDS 2237628 2237984 . - 0 Rv1994c cmtR Represses transcription from the CMT operator-promoter. Repression is alleviated by CD(II). Metal sensor transcriptional regulator CmtR (ArsR-SmtB family) Rv1994c, (MTCY39.25), len: 118 aa. CmtR, transcriptional regulator (See Cavet et al., 2003). Similar to MERR_STRLI|P30346 probable mercury resistance operon repressor (125 aa), FASTA scores: opt: 199, E(): 3e-08, (36.3% identity in 102 aa overlap). Note that primer extension analysis revealed two transcriptional start sites (See Chauhan et al., 2009). P9WMI9 No regulatory proteins 2JSC P67731 P9WMI9 GO:0046872,GO:0006355,GO:0006350,GO:0003700,GO:0005622 P9WMI9 MSMEG_5603 Mb2017c NC_000962.3 Mycobrowser_v4 promoter 2238001 2238036 . - 0 Rv1994c cmtR Represses transcription from the CMT operator-promoter. Repression is alleviated by CD(II). Metal sensor transcriptional regulator CmtR (ArsR-SmtB family) Rv1994c, (MTCY39.25), len: 118 aa. CmtR, transcriptional regulator (See Cavet et al., 2003). Similar to MERR_STRLI|P30346 probable mercury resistance operon repressor (125 aa), FASTA scores: opt: 199, E(): 3e-08, (36.3% identity in 102 aa overlap). Note that primer extension analysis revealed two transcriptional start sites (See Chauhan et al., 2009). P9WMI9 No regulatory proteins 2JSC P67731 P9WMI9 GO:0046872,GO:0006355,GO:0006350,GO:0003700,GO:0005622 P9WMI9 MSMEG_5603 Mb2017c NC_000962.3 Mycobrowser_v4 CDS 2239004 2239957 . + 0 Rv1996 Rv1996 Function unknown Universal stress protein family protein Rv1996, (MTCY39.23c), len: 317 aa. Universal stress protein family protein. Similar to several Mycobacterium tuberculosis hypothetical proteins e.g. Rv2005c|Q10851|YK05_MYCTU (295 aa), FASTA scores: opt: 775, E(): 0, (50.3% identity in 316 aa overlap); Rv2026c (294 aa) (47.9% identity in 311 aa overlap); and Rv2623, etc. Also similar to SCJ1.30c|AL109962 hypothetical protein from Streptomyces coelicolor (328 aa). Predicted possible vaccine candidate (See Zvi et al., 2008). P9WLP1 No virulence, detoxification, adaptation P0A5F7 P9WLP1 GO:0006950 P9WLP1 MMAR_5410 MSMEG_3945 Mb2019 NC_000962.3 Mycobrowser_v4 CDS 2240159 2242876 . + 0 Rv1997 ctpF Metal cation-transporting ATPase; possibly catalyzes the transport of an undetermined metal cation with the hydrolysis of ATP [catalytic activity: ATP + H(2)O + undetermined metal cation(in) = ADP + phosphate + undetermined metal cation(out)]. Probable metal cation transporter P-type ATPase A CtpF Rv1997, (MTCY39.22c, MTCY39.21c), len: 905 aa. Probable ctpF, metal cation-transporting P-type ATPase F (transmembrane protein), highly similar to others e.g. NP_250120.1|NC_002516 probable cation-transporting P-type ATPase from Pseudomonas aeruginosa (902 aa); NP_441217.1|NC_000911 cation-transporting ATPase (E1-E2 ATPase) from Synechocystis sp. strain PCC 6803 (905 aa); NP_404093.1|NC_003143 putative cation-transporting P-type ATPase from Yersinia pestis (908 aa); P37367|ATA1_SYNY3 cation-transporting ATPase pma1 from Synechocystis sp. (915 aa), FASTA scores: opt: 2392, E(): 0, (46.5% identity in 852 aa overlap); etc. Contains PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases), subfamily IB. Was frame-shifted in original cosmid sequence. P9WPS9 No cell wall and cell processes P63687 P9WPS9 GO:0006754,GO:0015662,GO:0006812,GO:0016021,GO:0046872,GO:0005886,GO:0005524 3.6.3.- P9WPS9 MMAR_0860 MSMEG_3926 Mb2020 NC_000962.3 Mycobrowser_v4 CDS 2242945 2243721 . - 0 Rv1998c Rv1998c Function unknown Conserved protein Rv1998c, (MTCY39.20), len: 258 aa. Conserved protein, showing some similarity with other hypothetical proteins e.g. U82823|SEU82823.03 Saccharopolyspora erythraea (266 aa), FASTA results: opt: 654, E(): 0, (43.8% identity in 249 aa overlap); and AL034446|SC1A9.07 Streptomyces coelicolor (251 aa), FASTA scores: opt: 592, E(): 1.5e-31, (43.4% identity in 251 aa overlap). P9WLN9 No conserved hypotheticals P9WLN9 GO:0003824 P9WLN9 Mb2021c NC_000962.3 Mycobrowser_v4 CDS 2245209 2246822 . + 0 Rv2000 Rv2000 Unknown Unknown protein Rv2000, (MTCY39.18c), len: 537 aa. Unknown protein. P9WLN7 No conserved hypotheticals P9WLN7 MMAR_2976 Mb2023 NC_000962.3 Mycobrowser_v4 CDS 2256617 2257942 . - 0 Rv2008c Rv2008c Unknown Conserved hypothetical protein Rv2008c, (MTCY39.09), len: 441 aa. Conserved hypothetical protein. Contains PS00017 ATP/GTP-binding site motif A, PS00501 Signal peptidases I serine active site. Also contains helix-turn-helix motif at aa 258-279. P9WLM9 No conserved hypotheticals P64923 P9WLM9 GO:0005524 P9WLM9 Mb2031c NC_000962.3 Mycobrowser_v4 CDS 2261816 2263072 . - 0 Rv2015c Rv2015c Unknown Conserved hypothetical protein Rv2015c, (MTV018.02c), len: 418 aa. Conserved hypothetical protein. Nearly identical to Mycobacterium tuberculosis Rv1765c|MTCY28.31c, (378 aa), an ORF starting next to ISB9, and ending in IS6110. Different N-terminus chosen and C-terminus differs as that of Rv1765c has been truncated by IS6110. Does not show similarities with transposases. Contains IPR002711 HNH endonuclease, IPR003615 HNH nuclease, IPR003870 DUF222 domains. O53461 No conserved hypotheticals O53461 O53461 GO:0003676,GO:0004519 Mb2038c NC_000962.3 Mycobrowser_v4 CDS 2265280 2265999 . + 0 Rv2018 Rv2018 Unknown Conserved protein Rv2018, (MTV018.05), len: 239 aa. Conserved protein. Contains probable helix-turn-helix motif at aa 215-236 (Score 1175, +3.19 SD). O53464 No conserved hypotheticals O53464 O53464 O53464 Mb2041 NC_000962.3 Mycobrowser_v4 CDS 2265989 2266405 . + 0 Rv2019 Rv2019 Unknown Conserved protein Rv2019, (MTV018.06), len: 138 aa. Conserved protein. O53465 No conserved hypotheticals O53465 Mb2042 NC_000962.3 Mycobrowser_v4 CDS 2266421 2266720 . - 0 Rv2020c Rv2020c Unknown Conserved hypothetical protein Rv2020c, (MTV018.07c), len: 99 aa. Conserved hypothetical protein, nearly identical to C-terminal part of hypothetical protein RvD1-Rv2024c' from Mycobacterium bovis BCG (1606 aa) emb|CAB44655.1| (Y18605). Corresponds to deletion region RvD1 so probably truncated protein. O53466 No conserved hypotheticals O53466 O53466 Mb2043c,Mb2049c NC_000962.3 Mycobrowser_v4 CDS 2266805 2267110 . - 0 Rv2021c Rv2021c Thought to be involved in transcriptional mechanism. Transcriptional regulatory protein Rv2021c, (MTV018.08c), len: 101 aa. Regulatory protein, similar to many. Contains probable helix-turn-helix at aa 45-66 (Score 1472, +4.20 SD); IPR001387 Helix-turn-helix type 3 domain. O53467 No regulatory proteins O53467 O53467 GO:0043565 Mb2044c NC_000962.3 Mycobrowser_v4 CDS 2321451 2322542 . + 0 Rv2064 cobG Required for cobalamin biosynthesis. Precorrin-3B synthase CobG Rv2064, (MTCY49.03), len: 363 aa. CobG, precorrin-3B synthase, cobalamin biosynthesis protein. Q10675 No intermediary metabolism and respiration Q10675 Q10675 GO:0016491,GO:0055114 1.14.13.83 Q10675 MMAR_3044 MSMEG_3871 NC_000962.3 Mycobrowser_v4 CDS 2268268 2268726 . - 0 Rv2023A Rv2023A Unknown Hypothetical protein, pseudogene Rv2023A, len: 152 aa. Hypothetical unknown protein (pseudogene), equivalent to the C-terminus of Q8VJS0|MT2080 hypothetical protein from Mycobacterium tuberculosis strain CDC1551 (225 aa), FASTA scores: opt: 1028, E(): 3.6e-66, (99.342% identity in 152 aa overlap) and C-terminus of Mb2047c hypothetical protein from Mycobacterium bovis (225 aa). And N-terminal part equivalent to the C-terminus of Q9XB17 hypothetical 15.5 kDa protein from Mycobacterium bovis BCG (131 aa), FASTA scores: opt: 409, E(): 4.2e-22, (98.276% identity in 58 aa overlap). Note that a deletion of DNA (RvD1 region) in Mycobacterium tuberculosis strain H37Rv resulted in a truncated CDS comparatively to Mycobacterium bovis or Mycobacterium tuberculosis strain CDC1551 genomes (see citations below). Yes conserved hypotheticals NC_000962.3 Mycobrowser_v4 CDS 2270750 2271748 . - 0 Rv2025c Rv2025c Unknown; possibly involved in transport of metal ions across the membrane. Conserved membrane protein Rv2025c, (MTV018.12c), len: 332 aa. Conserved transmembrane protein, involved in transport of metal ions, contains IPR002524 Cation efflux protein domain. P9WGF5 No cell wall and cell processes O53471 P9WGF5 GO:0006812,GO:0016020,GO:0055085,GO:0008324 P9WGF5 MMAR_3313 Mb2050c NC_000962.3 Mycobrowser_v4 CDS 2271863 2272747 . - 0 Rv2026c Rv2026c Unknown Universal stress protein family protein Rv2026c, (MTV018.13c), len: 294 aa. Universal stress protein family protein, contains IPR006016 UspA domain. P9WFD1 No virulence, detoxification, adaptation O53472 P9WFD1 GO:0006950 P9WFD1 MMAR_2995 MSMEG_3940,MSMEG_3950 Mb2051c NC_000962.3 Mycobrowser_v4 CDS 2275405 2276424 . - 0 Rv2029c pfkB Involved in glycolysis: converts sugar-1-P to sugar-1,6-P [catalytic activity: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate]. 6-phosphofructokinase PfkB (phosphohexokinase) (phosphofructokinase) Rv2029c, (MTV018.16c), len: 339 aa. PfkB, phosphofructokinase. Contains PS00583 pfkB family of carbohydrate kinases signature 1. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WID3 No intermediary metabolism and respiration O86352 P9WID3 GO:0016301,GO:0016773,GO:0005975 2.7.1.- P9WID3 MMAR_3482 MSMEG_3947 Mb2054c NC_000962.3 Mycobrowser_v4 CDS 2276441 2278486 . - 0 Rv2030c Rv2030c Unknown Conserved protein Rv2030c, (MTV018.17c), len: 681 aa. Conserved protein. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WLM1 No conserved hypotheticals O53475 P9WLM1 GO:0046677,GO:0016740,GO:0009116 P9WLM1 MMAR_3483 Mb2055c,Mb2056c NC_000962.3 Mycobrowser_v4 CDS 2278498 2278932 . - 0 Rv2031c hspX Stress protein induced by anoxia. Has a proposed role in maintenance of long-term viability during latent, asymptomatic infections, and a proposed role in replication during initial infection. Regulated by the two component regulatory system DEVR|Rv3133c/DEVS|Rv3132c, in response to a hypoxic signal. Heat shock protein HspX (alpha-crystallin homolog) (14 kDa antigen) (HSP16.3) Rv2031c, (MTV018.18c), len: 144 aa. HspX, heat shock protein localized in the inner membrane (see citations below). Identical to P30223|14KD_MYCTU 14 KD antigen (16 kDa antigen) (HSP 16.3) of Mycobacterium tuberculosis (143 aa). Belongs to the small heat shock protein (HSP20) family. Also known as alpha-crystallin and gene as acr (see some citations below). Predicted possible vaccine candidate (See Zvi et al., 2008). P9WMK1 No virulence, detoxification, adaptation P0A5B7 P9WMK1 GO:0005737,GO:0005576,GO:0005618 P9WMK1 MMAR_3484 MSMEG_3932 Mb2057c NC_000962.3 Mycobrowser_v4 CDS 2281294 2281617 . + 0 Rv2034 Rv2034 Involved in transcriptional regulation. ArsR repressor protein Rv2034, (MTV018.21), len: 107 aa. Repressor protein belonging to the ArsR family. Contains probable helix-turn-helix at aa 32-53 (S core 1350, +3.78 SD). O53478 No regulatory proteins O53478 O53478 GO:0006355,GO:0006350,GO:0003700,GO:0005622 O53478 MMAR_2816 Mb2060 NC_000962.3 Mycobrowser_v4 CDS 2281614 2282102 . + 0 Rv2035 Rv2035 Unknown Conserved hypothetical protein Rv2035, (MTV018.22), len: 162 aa. Conserved hypothetical protein, similar to many. Contains IPR013538 Activator of Hsp90 ATPase homologue 1-like. O53479 No conserved hypotheticals O53479 O53479 GO:0006950 O53479 Mb2061 NC_000962.3 Mycobrowser_v4 CDS 2283723 2284796 . - 0 Rv2038c Rv2038c Thought to be involved in active transport of sugar across the membrane (import). Responsible for energy coupling to the transport system. Probable sugar-transport ATP-binding protein ABC transporter Rv2038c, (MTV018.25c), len: 357 aa. Probable sugar-transport ATP-binding protein ABC transporter (see citation below), similar to many. Contains PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). O53482 No cell wall and cell processes O53482 O53482 GO:0043190,GO:0016887,GO:0016820,GO:0006810,GO:0005524