Refseq_ID Mycobrowser_Version Feature Start Stop Score Strand Frame Locus Name Function Product Comments UniProt_AC Is_Pseudogene Functional_Category Protein Data Bank PFAM UniProt Gene Ontology Enzyme Classification Drug Resistance Mutations InterPro UniProtKB/TrEMBL SWISS-MODEL Orthologues M. leprae Orthologues M. marinum Orthologues M. smegmatis Orthologues M. bovis Orthologues M. lepromatosis Orthologues M. tuberculosis Orthologues M. abscessus Orthologues M. haemophilum Orthologues M. orygis Orthologues M. tuberculosis NC_000962.3 Mycobrowser_v5 ncRNA 759479 759610 . + MTB000115 ncRv10666 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 786021 786074 . + MTB000116 ncRv10685 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 80240 80440 . + MTB000100 ncRv10071 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 80254 80344 . - MTB000101 ncRv10071c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 156452 156567 . + MTB000102 ncRv10128 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 156521 156568 . - MTB000103 ncRv10128c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 177236 177285 . - MTB000104 ncRv10150c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 209683 209841 . + MTB000105 ncRv0179 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 218320 218379 . - MTB000106 ncRv0186c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 293603 293663 . + MTB000107 ncRv10243 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 530246 530353 . - MTB000108 ncRv0441c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 558815 558884 . + MTB000109 ncRv10467 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 579290 579408 . + MTB000110 ncRv0490 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 704185 704246 . + MTB000111 ncRv10609 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 733361 733459 . + MTB000112 ncRv10637 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 734118 734244 . + MTB000113 ncRv0638 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 736166 736284 . + MTB000114 ncRv0641 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 800242 800359 . + MTB000117 ncRv10699 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 815417 815685 . + MTB000118 ncRv0724 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 905075 905164 . - MTB000119 ncRv0810c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 958459 958509 . + MTB000120 ncRv10860 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1000719 1000826 . + MTB000121 ncRv0897 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1063969 1064101 . + MTB000122 ncRv0952 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1113606 1113664 . + MTB000123 ncRv10996 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1165548 1165613 . - MTB000124 ncRv11042c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1197082 1197179 . + MTB000125 ncRv1072 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1271918 1271961 . - MTB000126 ncRv11144c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1275610 1275674 . - MTB000127 ncRv11147c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1313343 1313452 . - MTB000128 ncRv11179c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1342888 1342941 . + MTB000129 ncRv11199 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1821646 1821753 . - MTB000138 ncRv1621c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1960667 1960774 . + MTB000139 ncRv11733 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 2030986 2031038 . + MTB000140 ncRv11793 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 2068863 2068962 . + MTB000141 ncRv1821 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 2096766 2096867 . + MTB000142 ncRv11846 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 2268164 2268231 . + MTB000143 ncRv12023 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 2489205 2489252 . + MTB000144 ncRv12220 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 2762409 2762484 . + MTB000145 ncRv12459 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 2877751 2877808 . + MTB000146 ncRv12557 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 2966410 2966450 . + MTB000147 ncRv12641 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 3092761 3092886 . - MTB000148 ncRv12783c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 3343113 3343216 . - MTB000149 ncRv2986c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 3350950 3351074 . - MTB000150 ncRv2993c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 3363029 3363152 . - MTB000151 ncRv13003c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 3595951 3596059 . + MTB000152 ncRv3220 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 3690941 3691059 . + MTB000153 ncRv13303 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 3837346 3837458 . - MTB000155 ncRv13418cB Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 3880231 3880294 . - MTB000156 ncRv3461c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 3956291 3956550 . + MTB000157 ncRv3520 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 4088267 4088350 . - MTB000158 ncRv3648c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 4099384 4099477 . - MTB000159 ncRv13660c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 4168192 4168281 . - MTB000160 ncRv13722c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 4265583 4265765 . - MTB000161 ncRv3804c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 1446193 1446265 . - 0 MTB000018 argV - tRNA-Arg (CCG) argV, tRNA-Arg, anticodon ccg, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 1512728 1512811 . + 0 MTB000022 leuW - tRNA-Leu (TAG) leuW, tRNA-Leu, anticodon tag, length = 84 No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 1828015 1828088 . - 0 MTB000023 leuV - tRNA-Leu (CAA) leuV, tRNA-Leu, anticodon caa, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 2256084 2256428 . - 0 Rv2007c fdxA Involved in electron transfer. Ferredoxin FdxA Rv2007c, (MTCY39.10), len: 114 aa. FdxA, ferredoxin, similar to many e.g. FER_MYCSM P00215 ferredoxin, Mycobacterium smegmatis (106 aa). P9WNE7 No intermediary metabolism and respiration P64122 P9WNE7 GO:0051539,GO:0009055,GO:0022900,GO:0046872,GO:0006810,GO:0051538 P9WNE7 NC_000962.3 Mycobrowser_v5 CDS 3872617 3873405 . + 0 Rv3451 cut3 Hydrolysis of cutin (a polyester that forms the structure of plant cuticle). Shown to have TDM-specific hydrolase activity (see Yang et al. 2014). Probable cutinase precursor Cut3 Rv3451, (MTCY13E12.04), len: 262 aa. Probable cut3, cutinase precursor, similar to others e.g. Q9KK87 from Mycobacterium avium (220 aa), FASTA scores: opt: 540, E(): 3.5e-24, (43.4% identity in 219 aa overlap); Q00298|CUTI_BOTCI|CUTA from Botrytis cinerea (Botryotinia fuckeliana) (202 aa), FASTA scores: opt: 214, E(): 2e-05, (31.45% identity in 210 aa overlap); Q9Y7G8 from Pyrenopeziza brassicae (203 aa), FASTA scores: opt: 203, E(): 8.5e-05, (31.05% identity in 190 aa overlap); P29292|CUTI_ASCRA from Ascochyta rabiei (223 aa), FASTA scores: opt: 155, E(): 0.054, (31.65% identity in 120 aa overlap). Similar to other proteins from Mycobacterium tuberculosis e.g. the downstream ORF O06319|Rv3452|MTCY13E12.05 hypothetical 23.1 KDA protein (226 aa), FASTA scores: opt: 775, E(): 1e-37, (58.65% identity in 220 aa overlap); Q50664|CUT2_MYCTU|Rv2301|MT2358|MTCY339.08c probable cutinase precursor (219 aa), FASTA scores: opt: 565, E(): 1.3e-25, (44.85% identity in 223 aa overlap); Q10837|CUT1_MYCTU|Rv1984c|MT2037|MTCY39.35 probable cutinase precursor (217 aa), FASTA scores: opt: 489, E(): 3e-21, (47.05% identity in 221 aa overlap); etc. Equivalent to AAK47897 from Mycobacterium tuberculosis strain CDC1551 (247 aa) but longer 15 aa. Contains cutinase, serine active site motif (PS00155). Belongs to the cutinase family. Alternative start possible at 3733. Start changed since first submission (+15 aa). P9WP39 No cell wall and cell processes P0A536 P9WP39 GO:0005576,GO:0008152,GO:0050525 3.1.1.74 P9WP39 NC_000962.3 Mycobrowser_v5 CDS 3873452 3874132 . + 0 Rv3452 cut4 Hydrolysis of cutin (a polyester that forms the structure of plant cuticle). Shown to have esterase and phospholipase activity. Shown to have TDM-specific hydrolase activity (see Yang et al. 2014). Probable cutinase precursor Cut4 Rv3452, (MTCY13E12.05), len: 226 aa. Probable cut4, cutinase precursor, similar to other e.g. Q9KK87 from Mycobacterium avium (220 aa), FASTA scores: opt: 522, E(): 7.3e-24, (46.6% identity in 221 aa overlap); P30272|CUTI_MAGGR|CUT1 from Magnaporthe grisea (Rice blast fungus) (Pyricularia grisea) (228 aa), FASTA scores: opt: 205, E(): 3.8e-05, (29.25% identity in 164 aa overlap); Q00298|CUTI_BOTCI|CUTA from Botrytis cinerea (Botryotinia fuckeliana) (202 aa), FASTA scores: opt: 204, E(): 3.9e-05, (33.5% identity in 209 aa overlap); etc. Similar to other proteins from Mycobacterium tuberculosis e.g. upstream ORF O06318|CUT3_MYCTU|Rv3451|MT3557|MTCY13E12.04 probable cutinase precursor (247 aa), FASTA scores: opt: 773, E(): 1.3e-38, (59.35% identity in 209 aa overlap); Q50664|CUT2_MYCTU|Rv2301|MT2358|MTCY339.08c probable cutinase precursor (219 aa), FASTA scores: opt: 704, E(): 1.3e-34, (53.4% identity in 219 aa overlap); etc. Contains PS00155 Cutinase, serine active site. Belongs to the cutinase family. Alternative start possible at 4553 in cSCY13E12 but no RBS. O06319 No cell wall and cell processes O06319 O06319 GO:0008152,GO:0016787 3.1.1.- O06319 NC_000962.3 Mycobrowser_v5 CDS 2979326 2979688 . - 0 Rv2658c Rv2658c Unknown Possible prophage protein Rv2658c, (MTCY441.27c), len: 120 aa. Hypothetical unknown protein, probably phage protein. P9WL47 No insertion seqs and phages P9WL47 NC_000962.3 Mycobrowser_v5 CDS 2939959 2940612 . - 0 Rv2612c pgsA1 Catalyzes the transfer of a free alcohol (inositol) onto CDP-diacylglycerol. The product of this putative ORF seems be essential to mycobacteria [catalytic activity: CDP-diacylglycerol + myo-inositol = CMP + phosphatidyl 1D-myo-inositol]. PgsA1 utilises inositol-phosphate rather than inositol, in contrast to its Eukaryotic ortholog (see Gräve et al. 2019). PI synthase PgsA1 (phosphatidylinositol synthase) (CDP-diacylglycerol--inositol-3-phosphatidyltransferase) Rv2612c, (MTCY01A10.21), len: 217 aa. pgsA1 (previously known as pgsA), PI synthase/CDP-diacylglyceride--inositol phosphatidyltransferase, transmembrane protein, equivalent to O07149|MLCL581.16c|PGSA|ML0454 putative phosphatidyltransferase from Mycobacterium leprae (239 aa), FASTA scores: opt: 1141, E(): 4.1e-70, (79.35% identity in 213 aa overlap); and Q9F7Y9|PGSA phosphatidylinositol synthase from Mycobacterium smegmatis (222 aa), FASTA scores: opt: 981, E(): 2.7e-59, (67.3% identity in 217 aa overlap) (see citation below). Also similar to other proteins e.g. Q9L282|SCL2.17c putative membrane transferase from Streptomyces coelicolor (241 aa), FASTA scores: opt: 564, E(): 4.9e-31, (43.4% identity in 212 aa overlap); Q9UYD0|PGSA-like|PAB1041 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase from Pyrococcus abyssi (186 aa), FASTA scores: opt: 264, E(): 8.4e-11, (33.15% identity in 190 aa overlap); Q9HQS2|PGSA|VNG1030G CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase from Halobacterium sp. strain NRC-1 (199 aa), FASTA scores: opt: 249, E(): 9.1e-10, (32.1% identity in 193 aa overlap); etc. Contains PS00379 CDP-alcohol phosphatidyltransferases signature. Belongs to the CDP-alcohol phosphatidyltransferase class-I family. Note that in Mycobacterium smegmatis, the psgA homologue is essential to the survival of the bacteria and seems cannot be compensated by any other enzyme of Mycobacterium smegmatis. P9WPG7 No lipid metabolism Q7D6W6 P9WPG7 GO:0016021,GO:0008654,GO:0005886,GO:0003881 2.7.8.11 P9WPG7 NC_000962.3 Mycobrowser_v5 CDS 1950632 1951051 . - 0 Rv1724c Rv1724c Unknown Hypothetical protein Rv1724c, (MTCY04C12.09c), len: 139 aa. Hypothetical unknown protein. P71982 No conserved hypotheticals P71982 NC_000962.3 Mycobrowser_v5 CDS 2006636 2006947 . + 0 Rv1772 Rv1772 Unknown Hypothetical protein Rv1772, (MTCY28.38), len: 103 aa. Hypothetical unknown protein. O06805 No conserved hypotheticals O06805 O06805 INH NC_000962.3 Mycobrowser_v5 CDS 2012081 2012530 . - 0 Rv1778c Rv1778c Unknown Unknown protein Rv1778c, (MTCY25C11.05c), len: 149 aa. Unknown protein. O33181 No conserved hypotheticals O33181 NC_000962.3 Mycobrowser_v5 CDS 2159921 2160328 . - 0 Rv1914c Rv1914c Unknown Unknown protein Rv1914c, (MTCY180.04), len: 135 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). O07719 No conserved hypotheticals O07719 NC_000962.3 Mycobrowser_v5 CDS 2202584 2203129 . + 0 Rv1957 Rv1957 Unknown Hypothetical protein Rv1957, (MTCY09F9.07c), len: 181 aa. Hypothetical unknown protein. P95257 No conserved hypotheticals P95257 P95257 NC_000962.3 Mycobrowser_v5 CDS 2280240 2281082 . - 0 Rv2033c Rv2033c Unknown Conserved hypothetical protein Rv2033c, (MTV018.20), len: 280 aa. Conserved hypothetical protein, similar to many. O53477 No conserved hypotheticals O53477 O53477 NC_000962.3 Mycobrowser_v5 CDS 2287884 2288681 . - 0 Rv2042c Rv2042c Unknown Conserved protein Rv2042c, (MTV018.29c), len: 265 aa. Conserved protein, similar to many. O53486 No conserved hypotheticals O53486 O53486 GO:0006810,GO:0005622 O53486 NC_000962.3 Mycobrowser_v5 CDS 2327491 2328225 . - 0 Rv2070c cobK Involved in cobalamin biosynthesis. Precorrin-6X reductase CobK Rv2070c, (MTCY49.09c), len: 244 aa. CobK, precorrin-6x reductase, similar to many. P9WP89 No intermediary metabolism and respiration Q10680 P9WP89 GO:0055114,GO:0016994,GO:0009236 1.3.1.54 P9WP89 NC_000962.3 Mycobrowser_v5 CDS 2402507 2402722 . - 0 Rv2142A parD2 Unknown Possible antitoxin ParD2 Rv2142A, len: 71 aa. Possible parD2, antitoxin, part of toxin-antitoxin (TA) operon with Rv2142c (See Pandey and Gerdes, 2005). P9WJ75 No virulence, detoxification, adaptation P9WJ75 NC_000962.3 Mycobrowser_v5 tRNA 2835494 2835566 . + 0 MTB000034 lysU - tRNA-Lys (CTT) lysU, tRNA-Lys, anticodon ctt, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 2881252 2881320 . + 0 MTB000068 mpr11 Unknown Fragment of putative small regulatory RNA mpr11, fragment of putative small regulatory RNA (See DiChiara et al., 2010), ends not mapped, 82-100 nt band detected by Northern blot in M. bovis BCG Pasteur. No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 1138076 1138147 . - 0 MTB000016 glnT - tRNA-Gln (TTG) glnT, tRNA-Gln, anticodon ttg, length = 72 No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 1177396 1177469 . + 0 MTB000017 leuX - tRNA-Leu (TAA) leuX, tRNA-Leu, anticodon taa, length = 74. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1220388 1220487 . - 0 MTB000074 MTS0858 Unknown Putative small regulatory RNA MTS0858, putative small regulatory RNA (See Arnvig et al., 2011), ends not mapped, ~100 bp band detected by Northern blot. No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1535417 1535716 . - 0 MTB000065 mcr15 Unknown Putative small regulatory RNA mcr15, putative small regulatory RNA (See DiChiara et al., 2010). 5'-end mapped by 5'RLM-RACE in M. bovis BGC Pasteur, 3'-end not mapped. No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 2882185 2882276 . + 0 MTB000069 mpr12 Unknown Fragment of putative small regulatory RNA mpr12, fragment of putative small regulatory RNA (See DiChiara et al., 2010), ends not mapped, ~118 nt band detected by Northern blot in M. bovis BCG Pasteur. No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 2765331 2765404 . - 0 MTB000030 proU - tRNA-Pro (TGG) proU, tRNA-Pro; anticodon tgg, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 3020200 3020457 . - 0 Rv2706c Rv2706c Unknown Hypothetical protein Rv2706c, (MTCY05A6.27c), len: 85 aa (unlikely ORF). Hypothetical unknown protein. O07207 No conserved hypotheticals O07207 NC_000962.3 Mycobrowser_v5 CDS 3042542 3043018 . + 0 Rv2730 Rv2730 Unknown Hypothetical protein Rv2730, (MTCY174.10), len: 158 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6Y1K4 No conserved hypotheticals I6Y1K4 NC_000962.3 Mycobrowser_v5 CDS 3115408 3115719 . + 0 Rv2809 Rv2809 Unknown Hypothetical protein Rv2809, (MTCY16B7.34c), len: 103 aa (questionable ORF). Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6YEF3 No conserved hypotheticals I6YEF3 I6YEF3 NC_000962.3 Mycobrowser_v5 CDS 2926355 2926975 . + 0 Rv2597 Rv2597 Unknown Probable membrane protein Rv2597, (MTCY227.04c), len: 206 aa. Probable membrane protein. Predicted to be an outer membrane protein (See Song et al., 2008). P9WL73 No cell wall and cell processes P9WL73 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2962470 2962712 . + 0 Rv2635 Rv2635 Unknown Hypothetical protein Rv2635, (MTCY441.05), len: 80 aa. Hypothetical unknown protein. P9WL57 No conserved hypotheticals P9WL57 NC_000962.3 Mycobrowser_v5 CDS 2976586 2976909 . - 0 Rv2653c Rv2653c Unknown Possible PhiRv2 prophage protein Rv2653c, (MTCY441.22c), len: 107 aa. Hypothetical unknown protein, possibly phiRv2 phage protein (see citation below). P9WJ13 No insertion seqs and phages P9WJ13 NC_000962.3 Mycobrowser_v5 CDS 3366644 3367267 . + 0 Rv3008 Rv3008 Unknown Hypothetical protein Rv3008, (MTV012.22), len: 207 aa (start uncertain). Hypothetical unknown protein. I6YEY1 No conserved hypotheticals I6YEY1 NC_000962.3 Mycobrowser_v5 CDS 3392812 3393201 . + 0 Rv3032A Rv3032A Unknown Conserved protein Rv3032A, len: 129 aa. Conserved protein. I6X630 No conserved hypotheticals I6X630 NC_000962.3 Mycobrowser_v5 CDS 3540882 3541364 . - 0 Rv3172c Rv3172c Unknown Hypothetical protein Rv3172c, (MTV014.16c), len: 160 aa. Hypothetical unknown protein. O53322 No conserved hypotheticals O53322 O53322 NC_000962.3 Mycobrowser_v5 CDS 3611300 3611941 . + 0 Rv3235 Rv3235 Unknown Hypothetical alanine arginine proline rich protein Rv3235, (MTCY20B11.10), len: 213 aa. Hypothetical unknown ala-, arg-, pro-rich protein. O05880 No conserved hypotheticals O05880 O05880 NC_000962.3 Mycobrowser_v5 CDS 3908072 3908236 . + 0 Rv3489 Rv3489 Unknown Unknown protein Rv3489, (MTCY13E12.42), len: 54 aa. Unknown protein. No similarity with other proteins. I6YC91 No conserved hypotheticals I6YC91 I6YC91 NC_000962.3 Mycobrowser_v5 CDS 3909890 3910468 . + 0 Rv3491 Rv3491 Unknown Unknown protein Rv3491, (MTCY13E12.44), len: 192 aa. Unknown protein. No significant homology with other proteins. I6XHD1 No conserved hypotheticals I6XHD1 NC_000962.3 Mycobrowser_v5 CDS 3963605 3964054 . + 0 Rv3527 Rv3527 Unknown Hypothetical protein Rv3527, (MTCY03C7.29c), len: 149 aa. Hypothetical unknown protein. I6XHG6 No conserved hypotheticals I6XHG6 NC_000962.3 Mycobrowser_v5 CDS 4196724 4197107 . + 0 Rv3747 Rv3747 Function unknown Conserved protein Rv3747, (MTV025.095), len: 127 aa. Conserved protein, highly similar to downstream ORF O69715|Rv3748|MTV025.096 conserved hypothetical protein (119 aa), FASTA scores: opt: 494, E(): 6e-27, (64.4% identity in 118 aa overlap). O69714 No conserved hypotheticals O69714 NC_000962.3 Mycobrowser_v5 tRNA 2765541 2765611 . + 0 MTB000031 glyV - tRNA-Gly (TCC) glyV, tRNA-Gly; anticodon tcc, length = 71 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 3174747 3174995 . + 0 Rv2862A vapB23 Unknown Possible antitoxin VapB23 Rv2862A, len: 82 aa. Possible vapB23, antitoxin, part of toxin-antitoxin (TA) operon with Rv2863 (See Pandey and Gerdes, 2005). P0CW32 No virulence, detoxification, adaptation P0CW32 NC_000962.3 Mycobrowser_v5 CDS 4008167 4008433 . - 0 Rv3566A Rv3566A Unknown Hypothetical protein Rv3566A, len: 88 aa. Hypothetical unknown protein. I6YGI1 No conserved hypotheticals I6YGI1 NC_000962.3 Mycobrowser_v5 CDS 4042952 4043035 . - 0 Rv3599c Rv3599c Unknown Hypothetical short protein Rv3599c, (MTCY07H7B.23), len: 27 aa. Hypothetical unknown protein. O06283 No unknown O06283 NC_000962.3 Mycobrowser_v5 tRNA 3431840 3431912 . - 0 MTB000041 alaU - tRNA-Ala (GGC) alaU, tRNA-Ala, anticodon ggc, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 4081365 4081437 . - 0 MTB000044 thrU - tRNA-Thr (CGT) thrU, tRNA-Thr, anticodon cgt, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 2772098 2772331 . - 0 Rv2468A Rv2468A Unknown Conserved protein Rv2468A, len: 77 aa. Conserved protein. I6YDH3 No conserved hypotheticals I6YDH3 NC_000962.3 Mycobrowser_v5 CDS 4053881 4054042 . - 0 Rv3613c Rv3613c Unknown Hypothetical protein Rv3613c, (MTCY07H7B.09), len: 53 aa. Hypothetical unknown protein. O06270 No conserved hypotheticals O06270 NC_000962.3 Mycobrowser_v5 CDS 3708074 3708316 . - 0 Rv3321c vapB44 Unknown Possible antitoxin VapB44 Rv3321c, (MTV016.21c), len: 80 aa. Possible vapB44, antitoxin, part of toxin-antitoxin (TA) operon with Rv0299, see Arcus et al. 2005. Similar to several others in Mycobacterium tuberculosis (strains H37Rv and CDC1551) e.g. AAK48167|MT3800 DNA-binding protein (COPG family) from strain CDC1551 (74 aa), FASTA scores: opt: 142, E(): 0.0016, (48.85% identity in 43 aa overlap); AAK46916|MT2606 hypothetical 8.0 KDA protein from strain CDC1551 (74 aa), FASTA scores: opt: 139, E(): 0.0026, (37.2% identity in 78 aa overlap); O50456|Rv1241|MTV006.13 hypothetical 9.9 KDA protein from strain H37Rv (86 aa), FASTA scores: opt: 134, E(): 0.0066, (39.0% identity in 82 aa overlap); etc. P9WJ17 No virulence, detoxification, adaptation O53373 P9WJ17 GO:0006355,GO:0003677 NC_000962.3 Mycobrowser_v5 CDS 3598051 3598356 . - 0 Rv3221A rshA Binds sigma factor sigh and inhibits it. Probably involved in survival following heat shock and oxidative stress. Anti-sigma factor RshA Rv3221A, len: 101 aa. RshA, anti-sigma factor, similar to Q9XCD7|AAD41811.1 unknown protein from Mycobacterium smegmatis, linked to sigma factor sigH (see Fernandes et al., 1999) (101 aa), FASTA scores: opt: 422, E(): 3.4e-22, (64.9% identity in 94 aa overlap); and to Q9RL96|RsrA anti-sigma factor from Streptomyces coelicolor (see Kang et al., 1999) (105 aa), FASTA scores: opt: 163, E(): 0.00016, (32.05% identity in 78 aa overlap). P9WJ69 No information pathways P9WJ69 NC_000962.3 Mycobrowser_v5 CDS 3879273 3879692 . - 0 Rv3459c rpsK S11 plays an essential role for the selection of the correct tRNA in protein biosynthesis. It is located on the large lobe of the small subunit. 30S ribosomal protein S11 RpsK Rv3459c, (MTCY13E12.12c), len: 139 aa. rpsK, 30S ribosomal protein S11, equivalent to Q9X7A0|RS11_MYCLE|RPSK|ML1959|MLCB1222.29c 30S ribosomal protein S11 from Mycobacterium leprae (138 aa), FASTA scores: opt: 819, E(): 7.6e-44, (89.95% identity in 139 aa overlap); and P45812|RS11_MYCBO 30S ribosomal protein S11 from Mycobacterium bovis (139 aa), FASTA scores: opt: 867, E(): 8.4e-47, (94.25% identity in 139 aa overlap). Also highly similar to others e.g. P72403|RS11_STRCO|SC6G4.06 from Streptomyces coelicolor (134 aa), FASTA scores: opt: 729, E(): 2.6e-38, (79.85% identity in 139 aa overlap); O50633|RS11_BACHD|RPSK|BH0161 from Bacillus halodurans (129 aa), FASTA scores: opt: 618, E(): 1.7e-31, (70.3% identity in 128 aa overlap); P04969|RS11_BACSU|RPSK from Bacillus subtilis (131 aa), FASTA scores: opt: 601, E(): 2e-30, (69.0% identity in 129 aa overlap); etc. Contains ribosomal protein S11 signature (PS00054). Belongs to the S11P family of ribosomal proteins. P9WH65 No information pathways O06326 P9WH65 GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WH65 NC_000962.3 Mycobrowser_v5 ncRNA 4099386 4099478 . - 0 MTB000058 B11 Unknown Putative small regulatory RNA B11, putative small regulatory RNA (See Arnvig and Young, 2009; DiChiara et al., 2010). No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 547344 547517 . - 0 Rv0456B mazE1 Unknown Possible antitoxin MazE1 Rv0456B, len: 57 aa. Possible mazE1, antitoxin, part of toxin-antitoxin (TA) operon with Rv0456A (See Pandey and Gerdes, 2005; Zhu et al., 2006). P0CL57 No virulence, detoxification, adaptation P0CL57 NC_000962.3 Mycobrowser_v5 CDS 883451 884050 . - 0 Rv0789c Rv0789c Unknown Hypothetical protein Rv0789c, (MTCY369.33c), len: 199 aa. Hypothetical unknown protein. I6Y4U0 No conserved hypotheticals I6Y4U0 I6Y4U0 NC_000962.3 Mycobrowser_v5 CDS 3533913 3534395 . - 0 Rv3165c Rv3165c Unknown Unknown protein Rv3165c, (MTV014.09)c, len: 160 aa. Unknown protein. O53315 No conserved hypotheticals O53315 NC_000962.3 Mycobrowser_v5 CDS 474940 475344 . + 0 Rv0395 Rv0395 Unknown Hypothetical protein Rv0395, (MTCY04D9.07), len: 134 aa. Hypothetical unknown protein. P95203 No conserved hypotheticals P95203 NC_000962.3 Mycobrowser_v5 tRNA 658109 658189 . + 0 MTB000005 tyrT - tRNA-Tyr (GTA) tyrT, tRNA-Tyr, anticodon gta, length = 81 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 4013511 4014041 . + 0 Rv3572 Rv3572 Unknown Unknown protein Rv3572, (MTCY06G11.19), len: 176 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). I6X7P2 No conserved hypotheticals I6X7P2 NC_000962.3 Mycobrowser_v5 tRNA 733524 733596 . + 0 MTB000008 trpT - tRNA-Trp (CCA) trpT, tRNA-Trp, anticodon cca, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 1113511 1113583 . + 0 MTB000015 alaV - tRNA-Ala (CGC) alaV, tRNA-Ala, anticodon cgc, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 376573 377055 . - 0 Rv0307c Rv0307c Unknown Unknown protein Rv0307c, (MTCY63.12c), len: 160 aa. Unknown protein. O07234 No conserved hypotheticals O07234 O07234 NC_000962.3 Mycobrowser_v5 CDS 665042 665383 . - 0 Rv0572c Rv0572c Unknown Hypothetical protein Rv0572c, (MTV039.10c), len: 113 aa. Hypothetical unknown protein. P9WM81 No conserved hypotheticals P9WM81 NC_000962.3 Mycobrowser_v5 CDS 1280843 1281484 . - 0 Rv1154c Rv1154c Unknown Hypothetical protein Rv1154c, (MTCI65.21c), len: 213 aa. Hypothetical unknown protein, start uncertain. O06552 No conserved hypotheticals O06552 O06552 O06552 NC_000962.3 Mycobrowser_v5 CDS 2075571 2075828 . + 0 Rv1831 Rv1831 Unknown Hypothetical protein Rv1831, (MTCY1A11.12c), len: 85 aa. Hypothetical unknown protein. P9WLR3 No conserved hypotheticals P9WLR3 P9WLR3 NC_000962.3 Mycobrowser_v5 CDS 1735976 1736212 . + 0 Rv1535 Rv1535 Unknown Unknown protein Rv1535, (MTCY07A7A.04), len: 78 aa. Unknown protein. O06180 No conserved hypotheticals O06180 NC_000962.3 Mycobrowser_v5 CDS 2314661 2314825 . - 0 Rv2057c rpmG1 Involved in translation 50S ribosomal protein L33 RpmG1 Rv2057c, (MTCY63A.03), len: 54 aa. rpmG1, 50S ribosomal protein L33, similar to many. Note that previously known as rpmG. P9WH97 No information pathways P0A5W0 P9WH97 GO:0003735,GO:0006412,GO:0005840 P9WH97 NC_000962.3 Mycobrowser_v5 CDS 1612256 1612393 . + 0 Rv1434 Rv1434 Unknown Hypothetical protein Rv1434, (MTCY493.20c), len: 45 aa. Hypothetical unknown protein. O06824 No conserved hypotheticals O06824 NC_000962.3 Mycobrowser_v5 CDS 2201277 2201579 . + 0 Rv1954A Rv1954A Unknown Hypothetical protein Rv1954A, len: 100 aa. Hypothetical unknown protein. P0CV86 No unknown P0CV86 NC_000962.3 Mycobrowser_v5 tRNA 1946613 1946686 . + 0 MTB000024 proT - tRNA-Pro (GGG) proT, tRNA-Pro, anticodon ggg, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 2139419 2139656 . + 0 MTB000056 AS1890 Unknown Putative small regulatory RNA AS1890, putative small regulatory RNA (See Arnvig and Young, 2009). Alternate 5'-ends at positions 2139466, 2139548, 2139594. No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 2510598 2510669 . - 0 MTB000027 valV - tRNA-Val (TAC) valV, tRNA-Val, anticodon tac, length = 72 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 3082352 3082756 . - 0 Rv2774c Rv2774c Unknown Hypothetical protein Rv2774c, (MTV002.39c), len: 134 aa. Hypothetical unknown protein. O33316 No conserved hypotheticals O33316 O33316 NC_000962.3 Mycobrowser_v5 CDS 3502945 3503277 . - 0 Rv3136A Rv3136A Unknown Conserved protein Rv3136A, len: 110 aa. Conserved protein. I6Y2Q7 No conserved hypotheticals I6Y2Q7 I6Y2Q7 NC_000962.3 Mycobrowser_v5 CDS 3556855 3557064 . + 0 Rv3190A Rv3190A Unknown Conserved protein Rv3190A, len: 69 aa. Conserved protein. I6XGJ1 No conserved hypotheticals I6XGJ1 NC_000962.3 Mycobrowser_v5 CDS 3566696 3566896 . - 0 Rv3196A Rv3196A Unknown Unknown protein Rv3196A, len: 66 aa. Unknown protein. L7N668 No conserved hypotheticals L7N668 NC_000962.3 Mycobrowser_v5 tRNA 4168345 4168430 . + 0 MTB000046 serV - tRNA-Ser (GGA) serV, tRNA-Ser, anticodon gga, length = 86 No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 4317073 4317165 . + 0 MTB000080 MTS2975 Unknown Putative small regulatory RNA MTS2975, putative small regulatory RNA (See Arnvig et al., 2011), ends not mapped, ~100 bp band detected by Northern blot. No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 1774860 1775144 . - 0 Rv1567c Rv1567c Unknown Probable hypothetical membrane protein Rv1567c, (MTCY336.36), len: 94 aa. Probable membrane protein. O06623 No cell wall and cell processes O06623 NC_000962.3 Mycobrowser_v5 CDS 1815253 1815693 . + 0 Rv1615 Rv1615 Unknown Probable membrane protein Rv1615, (MTCY01B2.07), len: 146 aa. Probable membrane protein O06132 No cell wall and cell processes O06132 O06132 NC_000962.3 Mycobrowser_v5 CDS 1786307 1786528 . - 0 Rv1584c Rv1584c Unknown Possible PhiRv1 phage protein Rv1584c, (MTCY336.20), len: 73 aa. Possible phiRv1 phage protein (putative excisionase) (see citation below). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06606 No insertion seqs and phages O06606 NC_000962.3 Mycobrowser_v5 rRNA 1471846 1473382 . + 0 MTB000019 rrs - Ribosomal RNA 16S rrs, 16s rRNA gene (alternate gene name: rrnS). No stable RNAs AMI,SM NC_000962.3 Mycobrowser_v5 CDS 606149 606448 . + 0 Rv0514 Rv0514 Unknown Possible transmembrane protein Rv0514, (MTCY20G10.04), len: 99 aa. Possible transmembrane protein. O33359 No cell wall and cell processes O33359 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 710345 710587 . + 0 Rv0615 Rv0615 Unknown Probable integral membrane protein Rv0615, (MTCY19H5.06c), len: 80 aa. Probable integral membrane protein. O07762 No cell wall and cell processes O07762 NC_000962.3 Mycobrowser_v5 CDS 789157 789411 . - 0 Rv0689c Rv0689c Unknown Hypothetical protein Rv0689c, (MTCY210.06c), len: 84 aa. Hypothetical unknown protein. I6WZ39 No conserved hypotheticals I6WZ39 NC_000962.3 Mycobrowser_v5 tRNA 386204 386274 . - 0 MTB000004 glyU - tRNA-Gly (CCC) glyU, tRNA-Gly, anticodon ccc, length = 71 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 2546839 2547087 . - 0 Rv2274A mazE8 Unknown Possible antitoxin MazE8 Rv2274A, len: 82 aa. Possible mazE8, antitoxin, part of toxin-antitoxin (TA) operon with Rv2274c (See Pandey and Gerdes, 2005). P0CL60 No virulence, detoxification, adaptation P0CL60 NC_000962.3 Mycobrowser_v5 CDS 1003805 1003957 . + 0 Rv0900 Rv0900 Unknown Possible membrane protein Rv0900, (MTCY31.28), len: 50 aa. Possible membrane protein, with hydrophobic domain from aa 4-26. P9WJG7 No cell wall and cell processes P9WJG7 NC_000962.3 Mycobrowser_v5 CDS 2307293 2307517 . - 0 Rv2049c Rv2049c Unknown Conserved hypothetical protein Rv2049c, (MTV018.36c), len: 74 aa. Conserved hypothetical protein. O53491 No conserved hypotheticals O53491 NC_000962.3 Mycobrowser_v5 tRNA 850642 850713 . - 0 MTB000009 thrV - tRNA-Thr (TGT) thrV, tRNA-Thr, anticodon tgt, length = 72 No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 3348547 3348619 . - 0 MTB000039 gluU - tRNA-Glu (CTC) gluU, tRNA-Glu; anticodon ctc, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 186495 186623 . - 0 Rv0157A Rv0157A Unknown Conserved protein Rv0157A, len: 42 aa. Conserved protein, showing similarity to C-terminal part (aa 186-220) of O53976|Rv1975|MTV051.13 conserved hypothetical protein from Mycobacterium tuberculosis (221 aa), FASTA scores: opt: 173, E(): 3e-06, (62.5% identity in 40 aa overlap). I6WXK8 No conserved hypotheticals I6WXK8 NC_000962.3 Mycobrowser_v5 CDS 28362 29207 . + 0 Rv0024 Rv0024 Unknown. The P60 protein is a major extracellular protein may be involved in the invasion of host cells. Putative secreted protein P60-related protein Rv0024, (MTCY10H4.24), len: 281 aa. Putative secreted protein, p60 homologue, similar to many. Similar to Mycobacterium tuberculosis proteins Rv1477, Rv1478, Rv1566c, Rv2190c. Could belong to the E. coli NLPC / listeria P60 family. P71594 No virulence, detoxification, adaptation P71594 P71594 P71594 NC_000962.3 Mycobrowser_v5 CDS 1947416 1947643 . - 0 Rv1721c vapB12 Unknown Possible antitoxin VapB12 Rv1721c, (MTCY04C12.06c), len: 75 aa. Possible vapB12, antitoxin, part of toxin-antitoxin (TA) operon with Rv1720c (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others from Mycobacterium tuberculosis e.g. Rv0300|MTCY63.05|O07227 conserved hypothetical protein (73 aa). Start changed since original submission. P9WJ53 No virulence, detoxification, adaptation P9WJ53 P9WJ53 NC_000962.3 Mycobrowser_v5 CDS 4434 4997 . + 0 Rv0004 Rv0004 Function unknown Conserved hypothetical protein Rv0004, (MTCY10H4.02), len: 187 aa. Conserved hypothetical protein (see Salazar et al., 1996). Belongs to superfamily DUF721; this family contains several actinomycete proteins of unknown function. P9WFL1 No conserved hypotheticals P71573 P9WFL1 NC_000962.3 Mycobrowser_v5 CDS 2337306 2337869 . + 0 Rv2080 lppJ Unknown Lipoprotein LppJ Rv2080, (MTCY49.19), len: 187 aa. LppJ, lipoprotein; contains prokayotic lipoprotein modification site (PS00013) and signal sequence at N-terminus. P9WK77 No cell wall and cell processes P9WK77 GO:0005886 NC_000962.3 Mycobrowser_v5 CDS 42433 43365 . - 0 Rv0040c mtc28 Unknown Secreted proline rich protein Mtc28 (proline rich 28 kDa antigen) Rv0040c, (MTCY21D4.03c), len: 310 aa. Mtc28, secreted proline rich 28 kDa antigen protein (has hydrophobic stretch at N-terminus) (see citation below). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WIM9 No cell wall and cell processes P0A5Q6 P9WIM9 P9WIM9 NC_000962.3 Mycobrowser_v5 CDS 31514 31819 . + 0 Rv0028 Rv0028 Unknown Conserved hypothetical protein Rv0028, (MTCY10H4.28), len: 101 aa. Conserved hypothetical unknown protein. P9WM97 No conserved hypotheticals P9WM97 P9WM97 NC_000962.3 Mycobrowser_v5 ncRNA 1471619 1471742 . + 0 MTB000064 mcr3 Unknown Putative small regulatory RNA mcr3, putative small regulatory RNA (See DiChiara et al., 2010). 5'-end mapped by 5'RLM-RACE in M. bovis BGC Pasteur, 3'-end not mapped. No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 33224 33553 . + 0 Rv0030 Rv0030 Unknown Conserved hypothetical protein Rv0030, (MTCY10H4.30), len: 109 aa. Conserved hypothetical unknown protein. P9WM95 No conserved hypotheticals P9WM95 NC_000962.3 Mycobrowser_v5 CDS 33582 33794 . + 0 Rv0031 Rv0031 Normally, required for the transposition of an insertion element. Possible remnant of a transposase Rv0031, (MTCY10H4.31), len: 70 aa. Possible remnant of a transposase, showing partial similarity to mycobacterial transposases in a short overlap, e.g. Rv2791c|MTV002_57 (459 aa), FASTA score: (72.2% identity in 36 aa overlap); Rv2885c, Rv2978c, Rv3827c, etc. P71601 No insertion seqs and phages P71601 P71601 NC_000962.3 Mycobrowser_v5 CDS 51828 52697 . - 0 Rv0048c Rv0048c Unknown Possible membrane protein Rv0048c, MTCY21D4.11c, len: 289 aa. Possible membrane protein. P9WM87 No cell wall and cell processes P71705 P9WM87 GO:0005886,GO:0016021 P9WM87 NC_000962.3 Mycobrowser_v5 rRNA 1476899 1477013 . + 0 MTB000021 rrf - Ribosomal RNA 5S rrf, 5S rRNA gene. Identical to Em_ba:MT5SRR, D10035 M.tuberculosis 5S rRNA, len: 116. No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 86528 87133 . + 0 Rv0078 Rv0078 Possibly involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv0078, (MTV030.22), len: 201 aa. Probable transcriptional regulator. Contains probable helix-turn-helix motif from aa 35 to 56 (Score 1348, +3.78 SD). O53623 No regulatory proteins O53623 O53623 GO:0006350,GO:0003700,GO:0006355 O53623 NC_000962.3 Mycobrowser_v5 tRNA 10887 10960 . + 0 MTB000001 ileT - tRNA-Ile (GAT) ileT, tRNA-Ile, anticodon gat, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 11112 11184 . + 0 MTB000002 alaT - tRNA-Ala (TGC) alaT, tRNA-Ala, anticodon tgc, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 2123174 2123611 . + 0 Rv1873 Rv1873 Unknown Conserved hypothetical protein Rv1873, (MTCY180.45c), len: 145 aa. Conserved hypothetical protein. Some similarity to AL591783 hypothetical protein from Sinorhizobium meliloti. O07756 No conserved hypotheticals 2JEK O07756 O07756 O07756 NC_000962.3 Mycobrowser_v5 CDS 1160095 1160433 . - 0 Rv1036c Rv1036c Possibly required for the transposition of the insertion element IS1560. Probable IS1560 transposase (fragment) Rv1036c, (MTCY10G2.13), len: 112 aa. Probable IS1560 transposase fragment, similar to part of Rv3386|E1202304|MTV004.44 (234 aa) (82.8% identity in 87 aa overlap). P96365 No insertion seqs and phages P96365 P96365 NC_000962.3 Mycobrowser_v5 CDS 882524 882763 . + 0 Rv0787A Rv0787A Function unknown Conserved protein Rv0787A, len: 79 aa. Conserved protein, equivalent to MLCB5.24 hypothetical protein from Mycobacterium leprae (79 aa), FASTA scores: opt: 434, (84.8% identity in 79 aa overlap). Also similar to P12049|YEXA_BACSU hypothetical 9.7 kDa protein from Bacillus subtilis (84 aa), FASTA scores: opt: 172, E(): 4e-06, (44.4% identity in 72 aa overlap). Belongs to the UPF0062 family. I6Y8S6 No conserved hypotheticals Q8VKE0 I6Y8S6 GO:0016879 I6Y8S6 NC_000962.3 Mycobrowser_v5 CDS 2338065 2338505 . - 0 Rv2081c Rv2081c Unknown Conserved transmembrane protein Rv2081c, (MTCY49.20c), len: 146 aa. Conserved transmembrane protein, similar to many. Hydrophobic stretch from aa 32-54. P9WLK5 No cell wall and cell processes P9WLK5 GO:0005886,GO:0016021 P9WLK5 NC_000962.3 Mycobrowser_v5 CDS 1779194 1779298 . - 0 Rv1572c Rv1572c Unknown Conserved hypothetical protein Rv1572c, (MTCY336.31B), len: 34 aa. Partial ORF, part of REP13E12 repeat element; 3' end of Rv1587c (MTCY336.17) after phage-like element (see citation below). Similar to C-terminal ends of other REP13E12 repeat elements e.g. Rv1148, Rv1945, Rv3467, etc. Length extended since first submission (+7 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06618 No insertion seqs and phages O06618 NC_000962.3 Mycobrowser_v5 CDS 25913 26881 . - 0 Rv0021c Rv0021c Function unknown Conserved hypothetical protein Rv0021c, (MTCY10H4.21c), len: 322 aa. Conserved hypothetical protein, similar to various proteins. P71591 No conserved hypotheticals P71591 P71591 GO:0055114,GO:0018580 P71591 NC_000962.3 Mycobrowser_v5 CDS 476394 476642 . + 0 Rv0397A Rv0397A Unknown Conserved protein Rv0397A, len: 82 aa. Conserved protein. I6Y3N9 No conserved hypotheticals I6Y3N9 NC_000962.3 Mycobrowser_v5 CDS 1492320 1494425 . - 0 Rv1327c glgE Function unknown; probably involved in polysaccharides degradation. M1P-dependent maltosyltransferase GlgE Rv1327c, (MTCY130.12c), len: 701 aa. Probable glgE, glucanase, similar to AF172946|AF172946_2 putative glucanase GlgE from Mycobacterium smegmatis (697 aa), FASTA scores: opt: 3816, E(): 0, (78.5% identity in 692 aa overlap). Similar to putative alpha-amylases e.g. Q9L1K2 Streptomyces coelicolor (675 aa), FASTA scores: opt: 2243, E(): 7.4e-132, (54.2% identity in 684 aa overlap). Start changed since original submission (-36) based on similarity to GlgE of Mycobacterium smegmatis; previous start at position 1494531. P9WQ17 No intermediary metabolism and respiration P63531 P9WQ17 GO:0043169,GO:0016798,GO:0005975 3.2.1.- P9WQ17 NC_000962.3 Mycobrowser_v5 CDS 1 1524 . + 0 Rv0001 dnaA Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (DNAA box): 5'-TTATC(C/A)A(C/A)A-3'. DNAA binds to ATP and to acidic phospholipids. DNAA protein binds the origin of replication (oriC), ATP and ADP, and exhibited weak ATPase activity. Chromosomal replication initiator protein DnaA Rv0001, (MT0001, MTV029.01, P49993), len: 507 aa. dnaA, chromosomal replication initiator protein (see citations below), equivalent to other Mycobacterial chromosomal replication initiator proteins. Also highly similar to others except in N-terminus e.g. Q9ZH75|DNAA_STRCH chromosomal replication initiator protein from Streptomyces chrysomallus (624 aa). Contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS01008 DnaA protein signature. Belongs to the DnaA family. Note that the first base of this gene has been taken as base 1 of the Mycobacterium tuberculosis H37Rv genomic sequence. P9WNW3 No information pathways P49993 P9WNW3 GO:0003688,GO:0006270,GO:0005737,GO:0017111,GO:0006275,GO:0005524 P9WNW3 NC_000962.3 Mycobrowser_v5 CDS 2716395 2717138 . - 0 Rv2418c octT Transfers octanoate to glucosylglycerate (GG) and diglucosylglycerate (DGG), the earliest intermediates in methylglucose lipopolysaccharides (MGLP) biosynthesis (see Maranha et al. 2015). Sugar octanoyltransferase OctT Enzymatic studies, synthetic chemistry, NMR spectroscopy and mass spectrometry approaches suggest that, in contrast to the prevailing consensus, octanoate is not esterified to the primary hydroxyl group of glycerate but instead to the C6 OH of the second glucose in DGG (see Maranha et al. 2015). P71725 No conserved hypotheticals P71725 P71725 NC_000962.3 Mycobrowser_v5 CDS 2052 3260 . + 0 Rv0002 dnaN DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP-independent) along duplex DNA [catalytic activity: N deoxynucleoside triphosphate = N diphosphate + {DNA}N]. DNA polymerase III (beta chain) DnaN (DNA nucleotidyltransferase) Rv0002, (MTV029.02, MTCY10H4.0), len: 402 aa. DnaN, DNA polymerase III (beta chain) (see citations below), equivalent to other Mycobacterial DNA polymerases III beta chain. Also highly similar to others e.g. P27903|DP3B_STRCO DNA polymerase III beta chain from Streptomyces coelicolor (376 aa). Overlaps and extends CDS in neighbouring cosmid MTCY10H4.01. P9WNU1 No information pathways 3P16 Q50790 P9WNU1 GO:0003677,GO:0006260,GO:0003887,GO:0005737,GO:0008408 2.7.7.7 P9WNU1 NC_000962.3 Mycobrowser_v5 CDS 3280 4437 . + 0 Rv0003 recF The RECF protein is involved in DNA metabolism and recombination; it is required for DNA replication and normal sos inducibility. RECF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP. DNA replication and repair protein RecF (single-strand DNA binding protein) Rv0003, (MTCY10H4.01), len: 385 aa. RecF, DNA replication and repair protein (see citations below), equivalent to other mycobacterial DNA replication and repair proteins. Also highly similar to many others. Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00617 RecF protein signature 1, and PS00618 RecF protein signature 2. Belongs to the RecF family. P9WHI9 No information pathways Q59586 P9WHI9 GO:0006281,GO:0006260,GO:0009432,GO:0005737,GO:0003697,GO:0005524 P9WHI9 NC_000962.3 Mycobrowser_v5 CDS 7302 9818 . + 0 Rv0006 gyrA DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [catalytic activity: ATP-dependent breakage, passage and rejoining of double-stranded DNA]. DNA gyrase (subunit A) GyrA (DNA topoisomerase (ATP-hydrolysing)) (DNA topoisomerase II) (type II DNA topoisomerase) Rv0006, (MTCY10H4.04), len: 838 aa. GyrA, DNA gyrase subunit A (see citations below). Contains PS00018 EF-hand calcium-binding domain. P9WG47 No information pathways 3ILW,3IFZ Q07702 P9WG47 GO:0003918,GO:0006265,GO:0046677,GO:0005524 5.99.1.3 FLQ P9WG47 NC_000962.3 Mycobrowser_v5 CDS 9914 10828 . + 0 Rv0007 Rv0007 Unknown Possible conserved membrane protein Rv0007, (MTCY10H4.05), len: 304 aa. Possible conserved membrane protein. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WMA7 No cell wall and cell processes P71575 P9WMA7 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 11874 12311 . - 0 Rv0008c Rv0008c Unknown Possible membrane protein Rv0008c, (MTCY10H4.07c), len: 145 aa. Possible membrane protein. P9WJF3 No cell wall and cell processes P9WJF3 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 12468 13016 . + 0 Rv0009 ppiA PPIases accelerate the folding of proteins [catalytic activity: cis-trans isomerization of proline imidic peptide bonds in oligopeptides]. Probable iron-regulated peptidyl-prolyl cis-trans isomerase A PpiA (PPIase A) (rotamase A) Rv0009, (MTCY10H4.08), len: 182 aa. Probable ppiA (alternate gene name: cfp22), iron-regulated peptidyl-prolyl cis-trans isomerase A. Belongs to the cyclophilin-type PPIase family. Alternative start codon has been suggested. P9WHW3 No information pathways 1W74 P65762 P9WHW3 GO:0003755,GO:0006457,GO:0005737 5.2.1.8 P9WHW3 NC_000962.3 Mycobrowser_v5 CDS 583375 583704 . - 0 Rv0492A Rv0492A Unknown Hypothetical protein Rv0492A, len: 109 aa. Hypothetical unknown protein. GC plot suggests CDS. Q6MX36 No conserved hypotheticals Q6MX36 NC_000962.3 Mycobrowser_v5 CDS 13133 13558 . - 0 Rv0010c Rv0010c Unknown Probable conserved membrane protein Rv0010c, (MTCY10H4.10c), len: 141 aa. Probable conserved membrane protein. Belongs to superfamily DUF2581, conserved in the Actinomycetales. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WMA3 No cell wall and cell processes P71580 P9WMA3 GO:0005886,GO:0016021 P9WMA3 NC_000962.3 Mycobrowser_v5 CDS 13714 13995 . - 0 Rv0011c Rv0011c Unknown Probable conserved transmembrane protein Rv0011c, (MTCY10H4.11c), len: 93 aa. Probable conserved transmembrane protein. Belongs to uncharacterized protein family UPF0233. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WP57 No cell wall and cell processes P67376 P9WP57 GO:0005886,GO:0016021 P9WP57 NC_000962.3 Mycobrowser_v5 CDS 14089 14877 . + 0 Rv0012 Rv0012 Unknown Probable conserved membrane protein Rv0012, (MTCY10H4.12), len: 262 aa. Probable conserved membrane protein. Belongs to superfamily DUF881. Contains probable N-terminal signal sequence. L0T243 No cell wall and cell processes P71582 L0T243 L0T243 NC_000962.3 Mycobrowser_v5 CDS 15590 17470 . - 0 Rv0014c pknB Involved in signal transduction (via phosphorylation). Thought to regulate cell division/differentiation. Can phosphorylate the peptide substrate myelin basic protein (MBP) [catalytic activity: ATP + a protein = ADP + a phosphoprotein]. Transmembrane serine/threonine-protein kinase B PknB (protein kinase B) (STPK B) Rv0014c, (MTCY10H4.14c), len: 626 aa. PknB, transmembrane serine/threonine-protein kinase (see citations below). Contains PS00107 Protein kinases ATP-binding region signature, and PS00108 Serine/Threonine protein kinases active-site signature. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases. Experimental studies show evidence of auto-phosphorylation on serine/threonine residues. PknB has been shown to be a substrate for PstP and its kinase activity is affected by PstP-mediated dephosphorylation. PknB and PstP (Rv0018c) may act as a functional pair in vivo to control mycobacterial cell growth. P9WI81 No regulatory proteins 1MRU,1O6Y,2FUM,2KUD,2KUE,2KUF,2KUI,3F61,3F69 P0A5S4 P9WI81 GO:0046872,GO:0008658,GO:0006468,GO:0004674,GO:0005524 2.7.11.1 P9WI81 NC_000962.3 Mycobrowser_v5 CDS 18759 20234 . - 0 Rv0016c pbpA Involved in peptidoglycan synthesis (at the final stages). Cell wall formation; PBPA is supposed to be responsible for the determination of the rod shape of the cell. It synthesizes cross-linked peptidoglycan from lipid intermediates. Probable penicillin-binding protein PbpA Rv0016c, (MTCY10H4.16c), len: 491 aa. Probable pbpA, penicillin-binding protein, similar to many. P9WKD1 No cell wall and cell processes 3LO7 P71586 P9WKD1 GO:0016021,GO:0008658,GO:0009252,GO:0005886,GO:0008360,GO:0016740,GO:0007047 P9WKD1 NC_000962.3 Mycobrowser_v5 CDS 21637 23181 . - 0 Rv0018c pstP Involved in regulation (using dephosphorylation of a specific phosphorylated substrate). Phosphoserine/threonine phosphatase PstP Rv0018c, (MTCY10H4.18c), len: 514 aa. PstP, phosphoserine/threonine phosphatase. Experimental studies have shown that PstP specifically dephosporylates model phospho-Ser/Thr substrates and it is likely that PknB (Rv0014c) and PstP may act as a functional pair in vivo to control mycobacterial cell growth (See Boitel et al., 2003). P9WHW5 No regulatory proteins 1TXO,2CM1 P71588 P9WHW5 GO:0046872,GO:0004721,GO:0005886,GO:0016021 3.1.3.16 P9WHW5 NC_000962.3 Mycobrowser_v5 CDS 39877 41202 . - 0 Rv0037c Rv0037c Unknown; possibly involved in transport of macrolide across the membrane. Probable conserved integral membrane protein Rv0037c, (MTCY10H4.37c), len: 441 aa. Probable conserved integral membrane protein, member of major facilitator superfamily (MFS) possibly involved in transport of macrolide. P9WJY1 No cell wall and cell processes P0A5C1 P9WJY1 GO:0005886,GO:0055085,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 375914 376585 . + 0 Rv0306 Rv0306 Function unknown; probably involved in cellular metabolism. Putative oxidoreductase Rv0306, (MTCY63.11), len: 223 aa. Putative oxidoreductase, highly similar to H83485|9947208|AAG04663.1|AE004557_4|AE004557 conserved hypothetical protein from Pseudomonas aeruginosa strain PAO1 (218 aa); and to other putative oxidoreductases e.g. middle part of CAB76073.1|AL157953 putative nitroreductase from Streptomyces coelicolor (1212 aa); Q52685|BLUB protein involved in cobalamin (vitamin B12) synthesis from Rhodobacter capsulatus (206 aa), FASTA scores: opt: 318, E(): 2e-15, (35.6% identity in 191 aa overlap). O07233 No intermediary metabolism and respiration O07233 O07233 GO:0016491,GO:0055114 1.-.-.- O07233 NC_000962.3 Mycobrowser_v5 CDS 27595 28365 . + 0 Rv0023 Rv0023 Possibly involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0023, (MTCY10H4.23), len: 256 aa. Possible transcriptional regulator. Contains probable helix-turn helix motif from aa 19 to 40 (Score 1615, +4.69 SD). P9WMI3 No regulatory proteins P67704 P9WMI3 GO:0043565,GO:0006350,GO:0045449 P9WMI3 NC_000962.3 Mycobrowser_v5 CDS 29245 29607 . + 0 Rv0025 Rv0025 Function unknown Conserved hypothetical protein Rv0025, (MTCY10H4.25), len: 120 aa. Conserved hypothetical protein, showing some similarity to other proteins from Mycobacterium tuberculosis e.g. Rv0739 (268 aa), FASTA score: (37.6% identity in 101 aa overlap), and Rv0026 FASTA score: (35.4% identity in 113 aa overlap); etc. P9WMA1 No conserved hypotheticals P71595 P9WMA1 NC_000962.3 Mycobrowser_v5 CDS 29722 31068 . + 0 Rv0026 Rv0026 Function unknown Conserved hypothetical protein Rv0026, (MTCY10H4.26), len: 448 aa. Conserved hypothetical protein, showing some similarity to other proteins from Mycobacterium tuberculosis: Rv0025 FASTA score: (35.4% identity in 113 aa overlap) and Rv0739 (268 aa), FASTA score: (32.4% identity in 142 aa overlap). P9WMB1 No conserved hypotheticals P71596 P9WMB1 P9WMB1 NC_000962.3 Mycobrowser_v5 CDS 31189 31506 . + 0 Rv0027 Rv0027 Unknown Conserved hypothetical protein Rv0027, (MTCY10H4.27), len: 105 aa. Conserved hypothetical unknown protein. P9WM99 No conserved hypotheticals P64667 P9WM99 NC_000962.3 Mycobrowser_v5 CDS 32057 33154 . + 0 Rv0029 Rv0029 Function unknown Conserved hypothetical protein Rv0029, (MTCY10H4.29), len: 365 aa. Conserved hypothetical protein, showing some similarity to other proteins from Mycobacterium tuberculosis e.g. C-terminal region of Rv2082; Rv3899c. P71599 No conserved hypotheticals P71599 P71599 NC_000962.3 Mycobrowser_v5 CDS 34295 36610 . + 0 Rv0032 bioF2 Could be involved in biotin biosynthesis (at the first step) [catalytic activity: 6-carboxyhexanoyl-CoA + L-alanine = 8-amino-7-oxononanoate + CoA + CO2]. Possible 8-amino-7-oxononanoate synthase BioF2 (AONS) (8-amino-7-ketopelargonate synthase) (7-keto-8-amino-pelargonic acid synthetase) (7-KAP synthetase) (L-alanine--pimelyl CoA ligase) Rv0032, (MTCY10H4.32), len: 771 aa. Probable bioF2, 8-amino-7-oxononanoate synthase, with its C-terminal similar to others. Contains PS00599 Aminotransferases class-II pyridoxal-phosphate attachment site. Belongs to class-II of pyridoxal-phosphate-dependent aminotransferases. P9WQ85 No intermediary metabolism and respiration P71602 P9WQ85 GO:0008415,GO:0009058,GO:0016874,GO:0030170,GO:0008483,GO:0008710 2.3.1.47 P9WQ85 NC_000962.3 Mycobrowser_v5 CDS 36607 36870 . + 0 Rv0033 acpA Key component in de novo fatty acid biosynthesis. This protein is supposed to be the carrier of the growing fatty acid chain in fatty acid biosynthesis. Probable acyl carrier protein AcpA (ACP) Rv0033, (MTCY10H4.33), len: 87 aa. Probable acpA (alternate gene name: acpP), acyl carrier protein, similar to others. Also similar to proteins of Mycobacterium tuberculosis Rv1344 and Rv2244 (31.5% identity in 73 aa overlap). I6WX95 No lipid metabolism 2CGQ P71603 I6WX95 GO:0048037,GO:0000036 I6WX95 NC_000962.3 Mycobrowser_v5 CDS 36867 37262 . + 0 Rv0034 Rv0034 Function unknown Conserved hypothetical protein Rv0034, (MTCY10H4.34), len: 131 aa. Conserved hypothetical protein, showing weak similarity to AE001980|AE001980_7 hypothetical protein from Deinococcus radiodurans (120 aa), FASTA scores: opt: 141, E(): 0.0028, (29.3% identity in 123 aa overlap). P9WM93 No conserved hypotheticals P9WM93 P9WM93 NC_000962.3 Mycobrowser_v5 CDS 41304 41912 . + 0 Rv0038 Rv0038 Function unknown Conserved protein Rv0038, (MTCY10H4.38), len: 202 aa. Conserved protein, similar to many. P9WFK5 No conserved hypotheticals P67757 P9WFK5 P9WFK5 NC_000962.3 Mycobrowser_v5 CDS 42004 42351 . - 0 Rv0039c Rv0039c Unknown Possible conserved transmembrane protein Rv0039c, (MTCY21D4.02c, MTCY10H4.39c), len: 115 aa. Possible conserved transmembrane protein. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WM89 No cell wall and cell processes P9WM89 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 43562 46471 . + 0 Rv0041 leuS Involved in translation mechanism [catalytic activity: ATP + L-leucine + tRNA(LEU) = AMP + diphosphate + L-leucyl-tRNA(LEU)]. Probable leucyl-tRNA synthetase LeuS (leucine--tRNA ligase) (LEURS) Rv0041, (MTCY21D4.04), len: 969 aa. Probable leucyl-tRNA synthetase, similar to many. Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Belongs to class-I aminoacyl-tRNA synthetase family. P9WFV1 No information pathways P67510 P9WFV1 GO:0005737,GO:0004823,GO:0006429,GO:0005524 6.1.1.4 P9WFV1 NC_000962.3 Mycobrowser_v5 CDS 51185 51727 . - 0 Rv0047c Rv0047c Function unknown Conserved protein Rv0047c, (MTCY21D4.10c), len: 180 aa. Conserved protein, similar to many. P71704 No conserved hypotheticals P71704 P71704 P71704 NC_000962.3 Mycobrowser_v5 CDS 418949 419608 . + 0 Rv0349 Rv0349 Unknown Hypothetical protein Rv0349, (MTCY13E10.09), len: 219 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06300 No conserved hypotheticals O06300 NC_000962.3 Mycobrowser_v5 CDS 52831 53244 . + 0 Rv0049 Rv0049 Function unknown Conserved hypothetical protein Rv0049, (MTCY21D4.12), len: 137 aa. Conserved hypothetical protein. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WM85 No conserved hypotheticals P9WM85 NC_000962.3 Mycobrowser_v5 CDS 53663 55699 . + 0 Rv0050 ponA1 Involved in peptidoglycan synthesis (at the final stages), cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits). Probable bifunctional penicillin-binding protein 1A/1B PonA1 (murein polymerase) (PBP1): penicillin-insensitive transglycosylase (peptidoglycan TGASE) + penicillin-sensitive transpeptidase (DD-transpeptidase) Rv0050, (MTCY21D4.13), len: 678 aa. Probable ponA1, penicillin-binding protein (class A), bienzymatic protein with transglycosylase and transpeptidase activities (see Graham & Clark-Curtiss 1999), highly similar to many (see Billman-Jacobe et al., 1999). Belongs to the transglycosylase family in the N-terminal section, and to the transpeptidase family in the C-terminal section. P71707 No cell wall and cell processes P71707 P71707 GO:0016787,GO:0008658,GO:0009252,GO:0009274,GO:0008360,GO:0016757,GO:0007047 3.4.-.-,2.4.2.- P71707 NC_000962.3 Mycobrowser_v5 CDS 58192 58482 . + 0 Rv0053 rpsF Binds together with S18 to 16S ribosomal RNA. 30S ribosomal protein S6 RpsF Rv0053, (MTCY21D4.16), len: 96 aa. rpsF, 30S ribosomal protein S6, highly similar to many. Contains PS01048 Ribosomal protein S6 signature. Belongs to the S6P family of ribosomal proteins. P9WH31 No information pathways P66591 P9WH31 GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WH31 NC_000962.3 Mycobrowser_v5 CDS 63909 64967 . + 0 Rv0060 Rv0060 Function unknown Conserved hypothetical protein Rv0060, (MTV030.03), len: 352 aa. Conserved hypothetical protein. O53605 No conserved hypotheticals O53605 O53605 O53605 NC_000962.3 Mycobrowser_v5 CDS 65012 65350 . - 0 Rv0061c Rv0061c Unknown Hypothetical protein Rv0061c, len: 112 aa. Conserved hypothetical protein supported by RNA-seq data. Similar to MMAR_3839, 76% identity in 112 aa overlap. Replaces questionable ORF Rv0061 (MTV030.04). I6X8E6 No conserved hypotheticals I6X8E6 NC_000962.3 Mycobrowser_v5 CDS 65552 66694 . + 0 Rv0062 celA1 The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) exocellobiohydrolases that cut the dissaccharide cellobiose from the nonreducing end of the cellulose polymer chain; (3) beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose [catalytic activity:endohydrolysis of 1,4-beta-D-glucosidic linkages in cellulose]. Possible cellulase CelA1 (endoglucanase) (endo-1,4-beta-glucanase) (FI-cmcase) (carboxymethyl cellulase) Rv0062, (MTV030.05), len: 380 aa. Possible celA1, cellulase, similar to many. Seems to belong to cellulase family B (family 6 of glycosyl hydrolases). Note that previously known as celA. Q79G13 No intermediary metabolism and respiration 1UOZ,1UP0,1UP2,1UP3 Q79G13 Q79G13 GO:0030245,GO:0008810 3.2.1.4 Q79G13 NC_000962.3 Mycobrowser_v5 CDS 72274 74511 . - 0 Rv0066c icd2 Involved in the KREBS cycle [catalytic activity: isocitrate + NADP+ = 2-oxoglutarate + CO(2) + NADPH]. Probable isocitrate dehydrogenase [NADP] Icd2 (oxalosuccinate decarboxylase) (IDH) (NADP+-specific ICDH) (IDP) Rv0066c, (MTV030.09c), len: 745 aa. Probable icd2, isocitrate dehydrogenase NADP-dependent. Belongs to the monomeric-type family of IDH. Note that in H37Rv, Rv0066c is named icd2 and Rv3339c is icd1 while in CDC1551 and Erdman strains, Rv0066c is icd1 and Rv3339c is icd2. O53611 No intermediary metabolism and respiration O53611 O53611 GO:0046872,GO:0055114,GO:0006099,GO:0004450 1.1.1.42 O53611 NC_000962.3 Mycobrowser_v5 CDS 75301 76212 . + 0 Rv0068 Rv0068 Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv0068, (MTV030.11), len: 303 aa. Probable oxidoreductase, similar to many. O53613 No intermediary metabolism and respiration O53613 O53613 GO:0055114,GO:0016491 1.-.-.- O53613 NC_000962.3 Mycobrowser_v5 CDS 76237 77622 . - 0 Rv0069c sdaA Involved in gluconeogenesis from serine [catalytic activity: L-serine + H2O = pyruvate + NH3 + H2O]. Probable L-serine dehydratase SdaA (L-serine deaminase) (SDH) (L-SD) Rv0069c, (MTV030.12c), len: 461 aa. Probable sdaA, L-serine dehydratase. Belongs to the iron-sulfur dependent L-serine dehydratase family. Cofactor: iron-sulfur (4FE-4S) (probable). P9WGT5 No intermediary metabolism and respiration P66773 P9WGT5 GO:0003941,GO:0006094,GO:0046872,GO:0051539 4.3.1.17 P9WGT5 NC_000962.3 Mycobrowser_v5 CDS 81676 82668 . + 0 Rv0073 Rv0073 Thought to be involved in active transport of glutamine across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable glutamine-transport ATP-binding protein ABC transporter Rv0073, (MTV030.17), len: 330 aa. Probable glutamine-transport ATP-binding protein ABC-transporter (see citation below), similar to many ATP-binding proteins. Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00211 ABC transporters family signature, and PS00889 Cyclic nucleotide-binding domain signature 2. Belongs to the ATP-binding transport protein family (ABC transporters). Note that supposed act with near ORF Rv0072|MTV030.16 transmembrane ABC-transporter. P9WQK5 No cell wall and cell processes O53618 P9WQK5 GO:0016887,GO:0005952,GO:0008603,GO:0001932,GO:0005524 P9WQK5 NC_000962.3 Mycobrowser_v5 CDS 85636 86466 . - 0 Rv0077c Rv0077c Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv0077c, (MTV030.21c), len: 276 aa. Possible oxidoreductase, weakly similar to others from Streptomyces. Also similar to MTCY05A6_35 and MTCY1A11_10 from Mycobacterium tuberculosis. And shows some similarity in part with AAL17935.1|AY054120 putative epoxide hydrolase from Mycobacterium smegmatis (203 aa). O53622 No intermediary metabolism and respiration O53622 O53622 GO:0016491,GO:0055114 1.-.-.- O53622 NC_000962.3 Mycobrowser_v5 CDS 87208 87801 . - 0 Rv0078A Rv0078A Unknown Hypothetical protein Rv0078A, len: 197 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L7N686 No conserved hypotheticals Q8VKS0 L7N686 L7N686 NC_000962.3 Mycobrowser_v5 CDS 87798 88004 . - 0 Rv0078B Rv0078B Unknown Conserved protein Rv0078B, len: 68 aa. Conserved protein. I6X8G2 No conserved hypotheticals I6X8G2 I6X8G2 NC_000962.3 Mycobrowser_v5 CDS 88204 89025 . + 0 Rv0079 Rv0079 Unknown Unknown protein Rv0079, (MTV030.23), len: 273 aa. Unknown protein. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WMA9 No conserved hypotheticals P9WMA9 P9WMA9 NC_000962.3 Mycobrowser_v5 CDS 89022 89480 . + 0 Rv0080 Rv0080 Function unknown Conserved hypothetical protein Rv0080, (MTV030.24), len: 152 aa. Conserved hypothetical protein. Belongs to pyridoxine 5'-phosphate (PNP) oxidase-like (PNPOx-like) superfamily. P9WMA5 No conserved hypotheticals P9WMA5 P9WMA5 NC_000962.3 Mycobrowser_v5 CDS 90400 92322 . + 0 Rv0083 Rv0083 Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv0083, (MTV030.27, MTCY251.01), len: 640 aa. Probable oxidoreductase, showing some similarity to other various oxidoreductases. Nucleotide position 91071 in the genome sequence has been corrected, T:C resulting in I224I. P9WIW3 No intermediary metabolism and respiration Q10880 P9WIW3 GO:0008137,GO:0016021,GO:0005886,GO:0042773 NC_000962.3 Mycobrowser_v5 CDS 1211560 1213863 . + 0 Rv1087 PE_PGRS21 Function unknown PE-PGRS family protein PE_PGRS21 Rv1087, (MTV017.40), len: 767 aa. PE_PGRS21, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below). Similar to Rv1090|AL021897|MTV017_43 Mycobacterium tuberculosis H37Rv (853 aa), FASTA scores: opt: 2819, E(): 0, (59.8% identity in 860 aa overlap). Contains PS00583 pfkB family of carbohydrate kinases signature 1 near C -terminus. Predicted to be an outer membrane protein (See Song et al., 2008). Q79FT0 No PE/PPE Q79FT0 Q79FT0 Q79FT0 NC_000962.3 Mycobrowser_v5 CDS 98480 99250 . + 0 Rv0090 Rv0090 Unknown Possible membrane protein Rv0090, (MTCY251.08), len: 256 aa. Possible membrane protein. Contains IPR014511 Protein of unknown function DUF2068, transmembrane, subgroup. P9WM71 No cell wall and cell processes P64683 P9WM71 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 99684 100451 . + 0 Rv0091 mtn Responsible for cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH) [catalytic activity 1: methylthioadenosine + H2O = adenine + 5-methylthio-D-ribose] [catalytic activity 2: S-adenosyl-L-homocysteine + H2O = adenine + S-D-ribosyl-L-homocysteine]. Probable bifunctional MTA/SAH nucleosidase Mtn: 5'-methylthioadenosine nucleosidase (methylthioadenosine methylthioribohydrolase) + S-adenosylhomocysteine nucleosidase (S-adenosyl-L-homocysteine homocysteinylribohydrolase) Rv0091, (MTCY251.10), len: 255 aa. Probable mtn (alternate gene name: pfs), methylthioadenosine/S-Adenosylhomocysteine nucleosidase (MTA/SAH nucleosidase), including 5'-methylthioadenosine nucleosidase and S-adenosylhomocysteine nucleosidase, similar to others. Belongs to the MTN family. P9WJM3 No intermediary metabolism and respiration P67656 P9WJM3 GO:0008782,GO:0008930,GO:0009164,GO:0019509 3.2.2.9 P9WJM3 NC_000962.3 Mycobrowser_v5 CDS 100583 102868 . + 0 Rv0092 ctpA Cation-transporting ATPase; possibly catalyzes the transport of a cation (possibly copper) with the hydrolyse of ATP [catalytic activity: ATP + H(2)O + cation(in) = ADP + phosphate + cation(out)]. Cation transporter P-type ATPase a CtpA Rv0092, (MTCY251.11), len: 761 aa. CtpA, cation-transporting P-type ATPase a (transmembrane protein), highly similar to many. Contains PS01047 Heavy-metal-associated domain, and PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases), subfamily IB. P9WPU1 No cell wall and cell processes Q10876 P9WPU1 GO:0006754,GO:0004008,GO:0016021,GO:0046872,GO:0030001,GO:0005886,GO:0005524 3.6.3.- P9WPU1 NC_000962.3 Mycobrowser_v5 CDS 105324 106715 . + 0 Rv0096 PPE1 Function unknown PPE family protein PPE1 Rv0096, (MTCY251.15), len: 463 aa. PPE1, Member of the Mycobacterium tuberculosis PPE family, similar to many. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WI49 No PE/PPE Q10892 P9WI49 GO:0005886,GO:0016021 P9WI49 NC_000962.3 Mycobrowser_v5 CDS 108156 109778 . + 0 Rv0099 fadD10 Function unknown, but involvement in lipid degradation. Possible fatty-acid-CoA ligase FadD10 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv0099, (MTCY251.18), len: 540 aa. Possible fadD10, fatty-acid-CoA synthetase, similar to many. Contains PS00455 putative AMP-binding domain signature. Contains IPR000873 AMP-dependent synthetase/ligase domain. Belongs to the ATP-dependent AMP-binding enzyme family. P9WQ55 No lipid metabolism Q10878 P9WQ55 GO:0008152,GO:0016874 6.2.1.- P9WQ55 NC_000962.3 Mycobrowser_v5 CDS 110001 117539 . + 0 Rv0101 nrp Involved in lipid metabolism. Probable peptide synthetase Nrp (peptide synthase) Rv0101, (MTCY251.20), len: 2512 aa. Probable nrp, peptide synthetase, similar to others e.g. AAD44234.1|AF143772_40|PstB peptide synthetase from Mycobacterium avium (2552 aa); 7476034|S77657 cyclic peptide synthetase from Mycobacterium leprae (1401 aa), FASTA scores: opt: 4268, E(): 0, (65.7% identity in 1091 aa overlap); part of CAB55600.1|AJ238027 peptide synthetase from Mycobacterium smegmatis (5990). Also similar to e.g. AAD56240.1|AF184977_1|AF184977 DhbF protein from Bacillus subtilis (2378 aa); SRF1_BACSU|P27206 surfactin synthetase subunit 1 (3587 aa), FASTA scores: opt: 1708, E(): 0, (30.6% identity in 1633 aa overlap): etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), 2 x PS00455 Putative AMP-binding domain signature, and PS00012 Phosphopantetheine attachment site. Belongs to the ATP-dependent AMP-binding enzyme family. Thought to be not involved in mycobactin biosynthesis (see citation below). Q10896 No lipid metabolism Q10896 Q10896 GO:0000036,GO:0048037,GO:0016874,GO:0008152,GO:0031177,GO:0004043 6.-.-.- Q10896 NC_000962.3 Mycobrowser_v5 CDS 117714 119699 . + 0 Rv0102 Rv0102 Unknown Probable conserved integral membrane protein Rv0102, (MTCY251.21), len: 661 aa. Probable conserved integral membrane protein, highly similar to P53525|Y102_MYCLE|ML1998|NP_302349.1|NC_002677 possible membrane protein from Mycobacterium leprae (659 aa), FASTA scores: opt: 3107, E(): 0, (70.2% identity in 662 aa overlap). Also similar to others e.g. CAC01497.1|AL391017 putative integral membrane protein from Streptomyces coelicolor (316 aa); etc. Contains PS00343 Gram-positive cocci surface proteins 'anchoring' hexapeptide. P9WM63 No cell wall and cell processes P64689 P9WM63 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 124374 125570 . + 0 Rv0106 Rv0106 Function unknown Conserved hypothetical protein Rv0106, (MTCY251.25), len: 398 aa. Conserved hypothetical protein, similar to others e.g. AL049841|SCE9_33 from Streptomyces coelicolor (370 aa), FASTA scores: opt: 282, E(): 2.5e-11, (32.0% identity in 381 aa overlap); etc. Some similarity to P94400 homologue to nitrile hydratase region from Bacillus subtilis (397 aa), FASTA scores: opt: 226, E(): 5.4e-08, (26.4% identity in 405 aa overlap). Also similar to COBW_PSEDE|P29937 FASTA score: (25.3% identity in 186 aa overlap); and P47K_PSECL|P31521 47 kDa protein (p47k) (419 aa), FASTA score: (25.9% identity in 401 aa overlap). P9WPI5 No conserved hypotheticals P64691 P9WPI5 P9WPI5 NC_000962.3 Mycobrowser_v5 CDS 131382 132872 . + 0 Rv0109 PE_PGRS1 Function unknown PE-PGRS family protein PE_PGRS1 Rv0109, (MTV031.03c), len: 496 aa. PE_PGRS1, Member of the M. tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), highly similar to many e.g. Q50615|Y0DP_MYCTU hypothetical glycine-rich 40.8 kDa protein from Mycobacterium tuberculosis (498 aa), FASTA scores: opt: 1772, E(): 0, (57.3% identity in 513 aa overlap); etc. L0T2H7 No PE/PPE Q79G09 L0T2H7 L0T2H7 NC_000962.3 Mycobrowser_v5 CDS 137319 137909 . + 0 Rv0113 gmhA Involved in biosynthesis of nucleotide-activated glycero-manno-heptose: synthesis of glyceromannoheptose 7-phosphate (inner core lipopolysaccharide biosynthesis) [catalytic activity: D-sedoheptulose 7-phosphate = D-glycero-alpha,beta-D-manno-heptose 7-phosphate]. Probable sedoheptulose-7-phosphate isomerase GmhA (phosphoheptose isomerase) Rv0113, (MTV031.07), len: 196 aa. Probable gmhA (alternate gene name: lpcA), sedoheptulose-7-phosphate isomerase (see citation below), similar to many e.g. AE0005|HPAE000596_11 from Helicobacter pylori (192 aa), FASTA scores: opt: 451, E(): 1.9e-24, (45.1% identity in 162 aa overlap). Belongs to the sis family, LPCA subfamily. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WGG1 No cell wall and cell processes P0A604 P9WGG1 GO:0005975,GO:0005737,GO:0005529,GO:0008968 5.3.1.- P9WGG1 NC_000962.3 Mycobrowser_v5 CDS 137941 138513 . + 0 Rv0114 gmhB Involved in biosynthesis of nucleotide-activated glycero-manno-heptose. Involved in two pathways, D-alpha-D pathway [catalytic activity: D-glycero-alpha-D-manno-heptose 1,7-biphosphate = D-glycero-alpha-D-manno-heptose 1-phosphate] and L-beta-D pathway [catalytic activity: D-glycero-beta-D-manno-heptose 1,7-biphosphate = D-glycero-beta-D-manno-heptose 1-phosphate]. Possible D-alpha,beta-D-heptose-1,7-biphosphate phosphatase GmhB (D-glycero-D-manno-heptose 7-phosphate kinase) Rv0114, (MTV031.08), len: 190 aa. Possible gmhB, D-alpha,beta-D-heptose-1,7-biphosphate phosphatase (see citation below), similar to several hypothetical proteins and phosphatases e.g. HIS7_ECOLI|P06987 imidazoleglycerol-phosphate dehydratase (355 aa), FASTA scores: opt: 250, E(): 3.6e-11, (34.0 % identity in 141 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WMV3 No cell wall and cell processes P9WMV3 GO:0005737,GO:0000105,GO:0004401,GO:0005975 3.1.3.- P9WMV3 NC_000962.3 Mycobrowser_v5 CDS 142128 143876 . - 0 Rv0118c oxcA Involved in catabolism of oxalic acid [catalytic activity: oxalyl-CoA = formyl-CoA + CO2]. Probable oxalyl-CoA decarboxylase OxcA Rv0118c, (MTV031.12c), Len: 582 aa. Probable oxcA, oxalyl-CoA decarboxylase, highly similar to many e.g. P78093|OXC_ECOLI|7449483|B65011|YFDU|B2373|Z3637|ECS325 probable oxalyl-CoA decarboxylase from Escherichia coli (564 aa); M77128|OXAOXA_1 oxalyl-CoA decarboxylase from Oxalobacter formigenes (568 aa), FASTA scores: opt: 2124, E():0, (55.6% identity in 568 aa overlap). Also similar to mycobacterial IlvB proteins e.g. MLCB1788.46c unknown TPP-requiring enzyme from Mycobacterium leprae (548 aa); and AL0086|MLCB1788_19 from Mycobacterium leprae (548 aa), FASTA scores: opt: 831, E(): 0, (33.9% identity in 567 aa overlap). O53639 No intermediary metabolism and respiration O53639 O53639 GO:0033611,GO:0008949,GO:0030976,GO:0000287 4.1.1.8 O53639 NC_000962.3 Mycobrowser_v5 CDS 151148 152215 . + 0 Rv0125 pepA Function unknown; possibly hydrolyzes peptides and/or proteins (seems to cleave preferentially after serine residues). Probable serine protease PepA (serine proteinase) (MTB32A) Rv0125, (MTCI418B.07, MTB32A), len: 355 aa. Probable pepA (alternate gene name: mtb32a), serine protease (see Skeiky et al., 1999), highly similar to other proteases e.g. HHOB_ECOLI|P31137 protease hhob precursor (355 aa), FASTA scores: opt: 400, E(): 3.8e-14, (32.4% identity in 346 aa overlap). Also similar to Q50320 34 kDa protein precursor from Mycobacterium tuberculosis (361 aa), FASTA scores: opt: 1689, E(): 0, (70.7% identity in 362 aa overlap). Contains PS00135 Serine proteases, trypsin family, serine active site. Has a putative signal sequence at the N-terminus. Belongs to the serine protease family. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). O07175 No intermediary metabolism and respiration O07175 O07175 GO:0006508,GO:0004252,GO:0005515 3.4.21.- O07175 NC_000962.3 Mycobrowser_v5 CDS 152324 154129 . + 0 Rv0126 treS Involved in trehalose biosynthesis (protective effect). Converts maltose to trehalose. Mycobacteria can produce trehalose from glucose 6-phosphate and UDP-glucose (the OtsA-OtsB pathway) from glycogen-like alpha(1-->4)-linked glucose polymers (the TreY-TreZ pathway) and from maltose (the TreS pathway). Trehalose synthase TreS Rv0126, (MTCI418B.08), len: 601 aa. TreS, trehalose synthase (see citation below), highly similar to others e.g. CAA04601.2|AJ001205 putative trehalose synthase from Streptomyces coelicolor (566 aa); S71450|1536814|BAA11303.1|D78198 trehalose synthase maltose-specific from Pimelobacter sp. strain R48 (573 aa). Also similar to MAL1_DROME|P07191 possible maltase precursor (508 aa), FASTA scores: opt: 807, E(): 0, (33.7% identity in 504 aa overlap); and similar to proteins associated with amino-acid transport e.g. Q64319 rat protein which stimulates transport of cystine and dibasic and neutral amino acids (683 aa), FASTA scores: opt: 839, E(): 0, (32.0% identity in 531 aa overlap). Also similar to several other Mycobacterium tuberculosis proteins e.g. Rv2471 FASTA score: (31.7% identity in 164 aa overlap). P9WQ19 No virulence, detoxification, adaptation O07176 P9WQ19 GO:0043169,GO:0016853,GO:0005975 5.4.99.- P9WQ19 NC_000962.3 Mycobrowser_v5 CDS 166910 167290 . + 0 Rv0140 Rv0140 Function unknown Conserved protein Rv0140, (MTCI5.14), len: 126 aa. Conserved protein, similar to others e.g. P74567|D90916_48 hypothetical 20.8 KDP protein from Synechocystis sp. (180 aa), FASTA scores: opt: 229, E(): 4.7e-10, (36.1% identity in 108 aa overlap). Also similar to Rv1056 and Rv1670 from Mycobacterium tuberculosis. P96817 No conserved hypotheticals P96817 P96817 P96817 NC_000962.3 Mycobrowser_v5 CDS 156578 157600 . - 0 Rv0129c fbpC Proteins of the antigen 85 complex are responsible for the high affinity of mycobacteria to fibronectin. Possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity. Secreted antigen 85-C FbpC (85C) (antigen 85 complex C) (AG58C) (mycolyl transferase 85C) (fibronectin-binding protein C) Rv0129c, (MT0137, MTCI5.03c), len: 340 aa. FbpC (alternate gene names: mpt45, 85C, fbpC2), secreted antigen 85c (fibronectin-binding protein C) (mycolyl transferase 85C) (see citations below), also highly similar to other Mycobacterial antigen precursors e.g. A85C_MYCLE|Q05862 antigen 85-c precursor (85c) from Mycobacterium leprae (333 aa), FASTA scores: opt: 1937, E(): 0, (81.4% identity in 333 aa overlap); etc. P9WQN9 No lipid metabolism 1DQY,1DQZ,1VA5,3HRH P0A4V4 P9WQN9 GO:0005576,GO:0008415 2.3.1.- INH P9WQN9 NC_000962.3 Mycobrowser_v5 CDS 162644 163249 . - 0 Rv0135c Rv0135c Could be involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0135c, (MTCI5.09c), len: 201 aa. Possible transcriptional regulator, weakly similar to others e.g. P32398|YHGD_BACSU hypothetical transcriptional regulator from Bacillus subtilis (191 aa), FASTA scores: opt: 145, E(): 0.0012, (21.0% identity in 162 aa overlap). P96812 No regulatory proteins P96812 P96812 GO:0006350,GO:0003700,GO:0006355 P96812 NC_000962.3 Mycobrowser_v5 CDS 163366 164691 . + 0 Rv0136 cyp138 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Probable cytochrome P450 138 Cyp138 Rv0136, (MT0144, MTCI5.10), len: 441 aa. Probable cyp138, cytochrome P450 138, similar to others e.g. SLR0574|Q59990 from synechocystis SP. (444 aa), FASTA scores: opt: 315, E(): 1e-13, (25.7% identity in 416 aa overlap); etc. Also similar to MTV039_6 from Mycobacterium tuberculosis (472 aa), FASTA score: (38.2% identity in 442 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop); and PS00086 Cytochrome P450 cysteine heme-iron ligand signature. Belongs to the cytochrome P450 family. P9WPM3 No intermediary metabolism and respiration P63717 P9WPM3 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPM3 NC_000962.3 Mycobrowser_v5 CDS 164712 165260 . - 0 Rv0137c msrA Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine [catalytic activity: protein L-methionine + oxidized thioredoxin + H2O = protein-L-methionine-(S)-S-oxide + reduced thioredoxin]. Probable peptide methionine sulfoxide reductase MsrA (protein-methionine-S-oxide reductase) (peptide met(O) reductase) Rv0137c, (MTCI5.11c), len: 182 aa. Probable msrA, peptide methionine sulfoxide reductase (See St. John et al., 2001), equivalent to CAC32179.1|AL583926 putative peptide methionine sulfoxide from Mycobacterium leprae (177 aa). Highly similar to others e.g. CAC18703.1|AL451182 putative peptide methionine sulfoxide reductase from Streptomyces coelicolor (172 aa); PMSR_SCHPO|Q09859 putative peptide methionine sulfoxide reductase from Streptomyces (187 aa), FASTA scores: opt: 468, E(): 9.9e-26, (45.6% identity in 158 aa overlap); etc. Belongs to the MsrA family. P9WJM5 No intermediary metabolism and respiration 1NWA P0A5L0 P9WJM5 GO:0008113,GO:0006464,GO:0055114 1.8.4.11 P9WJM5 NC_000962.3 Mycobrowser_v5 CDS 167711 168637 . + 0 Rv0142 Rv0142 Function unknown Conserved hypothetical protein Rv0142, (MTCI5.16), len: 308 aa. Conserved hypothetical protein, similar, except in N-terminus, to AB88922.1|AL353862 hypothetical protein SCE34.20 from Streptomyces coelicolor (326 aa). P96819 No conserved hypotheticals P96819 GO:0003824,GO:0006281 P96819 NC_000962.3 Mycobrowser_v5 CDS 168704 170182 . - 0 Rv0143c Rv0143c Unknown; possibly ion channel involved in transport of chloride across the membrane. Probable conserved transmembrane protein Rv0143c, (MTCI5.17c), len: 492 aa. Probable conserved transmembrane protein, CIC family possibly involved in transport of chloride, similar to others and hypothetical proteins e.g. O28857 putative chloride channel from Archaeoglobus fulgidus (589 aa), FASTA scores: opt: 966, E(): 0, (37.7% identity in 453 aa overlap); YADQ_ECOLI|P37019 hypothetical 46.0 kDa protein (436 aa), FASTA scores: opt: 452, E(): 2.4e-20, (28.0% identity in 460 aa overlap). P96820 No cell wall and cell processes P96820 P96820 GO:0016021,GO:0055085,GO:0005247,GO:0006821 P96820 NC_000962.3 Mycobrowser_v5 CDS 173238 174758 . + 0 Rv0147 Rv0147 Function unknown; probably involved in cellular metabolism [catalytic activity: an aldehyde + NAD+ + H2O = an acid + NADH]. Probable aldehyde dehydrogenase (NAD+) dependent Rv0147, (MTCI5.21), len: 506 aa. Probable aldehyde dehydrogenase (NAD+) dependent, similar to others e.g. DHAP_RAT|P11883 aldehyde dehydrogenase (dimeric NADP-preferring) (452 aa), FASTA scores: opt: 1291, E(): 0, (43.9% identity in 453 aa overlap). Also similar to several Mycobacterium tuberculosis aldehyde dehydrogenases e.g. Rv0768, Rv2858c, etc. Contains PS00687 aldehyde dehydrogenases glutamic acid active site, and PS00070 aldehyde dehydrogenases cysteine active site. Belongs to the aldehyde dehydrogenases family. P96824 No intermediary metabolism and respiration P96824 P96824 GO:0006081,GO:0055114,GO:0004030 1.2.1.- P96824 NC_000962.3 Mycobrowser_v5 CDS 177543 179309 . - 0 Rv0151c PE1 Function unknown PE family protein PE1 Rv0151c, (MTCI5.25c), len: 588 aa. PE1, Member of the Mycobacterium tuberculosis PE family (see citation below), with N-terminal region similar to others e.g. MTV032_2 PE_PGRS family from Mycobacterium tuberculosis (468 aa), FASTA scores: opt: 1125, E(): 0, (46.3% identity in 456 aa overlap); MTCY493_24 from Mycobacterium tuberculosis FASTA score: (42.5% identity in 558 aa overlap). Also similar to upstream ORF MTCI5.26c FASTA score: (54.7% identity in 464 aa overlap). Also shows similarity to C-terminal part of some PPE family proteins e.g. MTV049_21 from Mycobacterium tuberculosis FASTA score: (41.5% identity in 591 aa overlap). Q79G06 No PE/PPE Q79G06 Q79G06 Q79G06 NC_000962.3 Mycobrowser_v5 CDS 179319 180896 . - 0 Rv0152c PE2 Function unknown PE family protein PE2 Rv0152c, (MTCI5.26c), len: 525 aa. PE2, Member of the Mycobacterium tuberculosis PE family (see citation below), similar to ORF downstream Z92770|MTCI5_25 (588 aa), FASTA scores: opt: 1492, E(): 0, (54.7% identity in 464 aa overlap); and to many other PE family type members. Predicted to be an outer membrane protein (See Song et al., 2008). Q79G05 No PE/PPE Q79G05 Q79G05 Q79G05 NC_000962.3 Mycobrowser_v5 CDS 181155 181985 . - 0 Rv0153c ptbB Involved in signal transduction (via dephosphorylation). Can dephosphorylate in vitro the phosphotyrosine residue of myelin basic protein (MBP) at pH 7.0. Could be involved in virulence by interfering with phosphotyrosine-mediated signals in macrophages. Also able to dephosphorylate phosphoserine/threonine peptides and phosphoinositide substrates. [catalytic activity: protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate] Phosphotyrosine protein phosphatase PTPB (protein-tyrosine-phosphatase) (PTPase) Rv0153c, (MTCI5.27c), len: 276 aa. PtbB (alternate gene name: MPtpB), protein-tyrosine-phosphatase (see citation below), showing some similarity to several protein-tyrosine phosphatases, polyketide synthase and aminotransferase e.g. Q05918|IPHP_NOSCO|IPH protein-tyrosine-phosphatase precursor from Nostoc commune (294 aa), FASTA scores: opt: 150, E(): 0.0096, (26.8% identity in 269 aa overlap); etc. Supposedly a secreted protein. Potent and selective inhibitor is an isoxazole compound (See Seollner et al., 2007). I6WXK4 No regulatory proteins 1YWF,2OZ5 I6WXK4 GO:0004725,GO:0016311 3.1.3.48 I6WXK4 NC_000962.3 Mycobrowser_v5 CDS 185052 186479 . + 0 Rv0157 pntB The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [catalytic activity: NADPH + NAD+ = NADP+ + NADH]. Probable NAD(P) transhydrogenase (subunit beta) PntB [integral membrane protein] (pyridine nucleotide transhydrogenase subunit beta) (nicotinamide nucleotide transhydrogenase subunit beta) Rv0157, (MTCI5.31), len: 475 aa. Probable pntB, pyridine nucleotide transhydrogenase (nicotinamide nucleotide transhydrogenase) subunit beta, integral membrane protein, similar to others e.g. Q59763 proton-translocating nicotinamide nucleotide transhydrogenase subunit beta from hodospirillum rubrum (464 aa), FASTA scores: opt: 1344, E(): 0, (46.4% identity in 472 aa overlap); P07002|PNTB_ECOLI|P76890|PNTB|B1602|Z2597|ECS2308 NAD(P) transhydrogenase subunit beta from Escherichia coli strains K12 and O157:H7 (462 aa). P96834 No intermediary metabolism and respiration P96834 P96834 GO:0050661,GO:0016021,GO:0055114,GO:0008750 1.6.1.2 P96834 NC_000962.3 Mycobrowser_v5 CDS 190607 191956 . + 0 Rv0161 Rv0161 Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0161, (MTCI28.01, MTV032.04), len: 449 aa. Possible oxidoreductase, similar to hypothetical proteins and various oxidoreductases e.g. AIP2_YEAST|P46681 actin interacting protein 2 (530 aa), FASTA scores: opt: 356, E (): 0, (33.3% identity in 357 aa overlap); DLD1_YEAST|P32891 d-lactate dehydrogenase (cytochrome) (587 aa), FASTA scores: opt: 311, E(): 2.5e-20, (27.9% identity in 366 aa overlap). Also similar to other Mycobacteria proteins e.g. MTCY339.30c from Mycobacterium tuberculosis FASTA score: (29.4% identity in 357 aa overlap); MLCL622.30c from Mycobacterium tuberculosis (449 aa). O07406 No intermediary metabolism and respiration O07406 O07406 GO:0055114,GO:0016491,GO:0050660 1.-.-.- O07406 NC_000962.3 Mycobrowser_v5 CDS 194993 196657 . + 0 Rv0166 fadD5 Function unknown, but involved in lipid degradation. Probable fatty-acid-CoA ligase FadD5 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv0166, (MTCI28.06), len: 554 aa. Probable fadD5, fatty-acid-CoA synthetase, similar to many eg LCFA_ECOLI|P29212 long-chain-fatty-acid--CoA ligase (561 aa), FASTA scores: opt: 612, E(): 0, (29.4% identity in 534 aa overlap). Also similar to many other fatty-acid-CoA ligases from Mycobacterium tuberculosis e.g. MTCY07A7.11c FASTA score: (35.3% identity in 487 aa overlap), MTV013_10, MTY25D10_30, etc. Contains PS00455 putative AMP-binding domain signature. O07411 No lipid metabolism O07411 O07411 GO:0008152,GO:0016874 6.2.1.- O07411 NC_000962.3 Mycobrowser_v5 CDS 198534 199898 . + 0 Rv0169 mce1A Unknown, but thought to be involved in host cell invasion (entry and survival inside macrophages). Mce-family protein Mce1A Rv0169, (MTCI28.09), len: 454 aa. Mce1A; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07789|MCE2|Rv0589|MTCY19H5.33c|mce2A (404 aa); O53967|MCE3|Rv1966|MTV051.04|mce3A (425 aa); etc. Also highly similar to others e.g. AAD52105.1|AF113402_1|AF113402 mycobacterial cell entry protein from Mycobacterium bovis BCG (454 aa); NP_302656.1|NC_002677 putative cell invasion protein from Mycobacterium leprae (441 aa); AAA92845.1|U26018 mce gene product from Mycobacterium avium (88 aa) (similarity on C-terminus); CAC12798.1|AL445327 putative secreted protein from Streptomyces coelicolor (418 aa); etc. Note that equivalent, but longer 22 aa, to P72013|CAA50257.1|X70901 Mcep protein from Mycobacterium tuberculosis (432 aa). Contains a very hydrophobic region around residues 20-35. Note that previously known as mce1. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). Q79FZ9 No virulence, detoxification, adaptation Q79FZ9 Q79FZ9 Q79FZ9 NC_000962.3 Mycobrowser_v5 CDS 200932 202479 . + 0 Rv0171 mce1C Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce1C Rv0171, (MTCI28.11), len: 515 aa. Mce1C; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07787|Rv0591|MTCY19H5.31|mce2C (481 aa); O53969|Rv1968|MTV051.06|mce3C (410 aa); etc. Also highly similar to others e.g. NP_302658.1|NC_002677 putative secreted protein from Mycobacterium leprae (519 aa); CAC12796.1|AL445327 putative secreted protein from Streptomyces coelicolor (351 aa); etc. Weakly similar to downstream ORF Rv0172|MTCI28.12|mce1D (530 aa), FASTA score: (24.6% identity in 552 aa overlap). Contains possible signal sequence and highly proline-rich C-terminus. Predicted to be an outer membrane protein (See Song et al., 2008). O07415 No virulence, detoxification, adaptation O07415 O07415 O07415 NC_000962.3 Mycobrowser_v5 CDS 205231 206778 . + 0 Rv0174 mce1F Unknown, but thought involved in host cell invasion. Mce-family protein Mce1F Rv0174, (MTCI28.14), len: 515 aa. Mce1F; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), similar to Mycobacterium tuberculosis proteins O07784|Rv0594|MTCY19H5.28c|mce2F (516 aa); O53972|Rv1971|MTV051.09|mce3F (437 aa); etc. Also highly similar to others e.g. NP_302661.1|NC_002677 putative secreted protein from Mycobacterium leprae (516 aa); AAF74993.1|AF143400_1|AF143400|996A027a protein from Mycobacterium avium (80 aa) (similarity on C-terminus); CAC12793.1|AL445327 putative secreted protein from Streptomyces coelicolor (433 aa); etc. Has hydrophobic stretch, possibly a signal peptide at the N-terminus. Predicted to be an outer membrane protein (See Song et al., 2008). L0T2W6 No virulence, detoxification, adaptation O07418 L0T2W6 L0T2W6 NC_000962.3 Mycobrowser_v5 CDS 208938 209672 . + 0 Rv0178 Rv0178 Unknown Probable conserved Mce associated membrane protein Rv0178, (MTCI28.18), len: 244 aa. Probable conserved Mce-associated membrane protein, highly similar in C-terminus to CAC32130.1|AL583926 putative secreted protein from Mycobacterium leprae (184 aa). Also similar to mce-associated proteins from Mycobacterium tuberculosis e.g. Rv1363c, Rv0177, Rv1973, etc. Note that there is a 10 aa overlap with the upstream ORF. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O07422 No cell wall and cell processes O07422 O07422 NC_000962.3 Mycobrowser_v5 CDS 215715 216224 . + 0 Rv0185 Rv0185 Function unknown; probably involved in a cellular metabolism. Conserved hypothetical protein Rv0185, (MTCI28.25a), len: 169 aa. Conserved hypothetical protein, equivalent to CAB08794.1|Z95398|MLCL622_2 from Mycobacterium leprae (168 aa), FASTA scores: opt: 861, E(): 0, (76.4% identity in 165 aa overlap). Contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O07429 No conserved hypotheticals O07429 NC_000962.3 Mycobrowser_v5 CDS 237206 237895 . + 0 Rv0200 Rv0200 Unknown Possible conserved transmembrane protein Rv0200, (MTV033.08), len: 229 aa. Possible conserved transmembrane protein, equivalent to Z95398|MLCL622.14 from Mycobacterium leprae (229 aa), FASTA scores: opt: 1147, E(): 0, (74.7% identity in 229 aa overlap). Also some similarity to Rv1973 from Mycobacterium tuberculosis (160 aa); and Rv1362c|Z75555|MTCY02B10_26 (220 aa), FASTA scores: opt: 134, E(): 0.063, (25.8% identity in 159 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53651 No cell wall and cell processes O53651 GO:0016021 O53651 NC_000962.3 Mycobrowser_v5 CDS 219996 221723 . - 0 Rv0189c ilvD Involved in valine and isoleucine biosynthesis (at the fourth step) [catalytic activity: 2,3-dihydroxy-3-methylbutanoate = 3-methyl-2- oxobutanoate + H(2)O]. Probable dihydroxy-acid dehydratase IlvD (dad) Rv0189c, (MTCI28.28c), len: 575 aa. Probable ilvD, dihydroxy-acid dehydratase, similar to many e.g. ILVD_LACLA|Q02139 dihydroxy-acid dehydratase (dad) from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (570 aa), FASTA scores: opt: 1605, E(): 0, (46.0% identity in 561 aa overlap). Also similar to ML2608|MLCL622.06c|O06069|ILVD_MYCLE dihydroxy-acid dehydratase from Mycobacterium leprae (564 aa). Contains PS00886 Dihydroxy-acid and 6-phosphogluconate dehydratases signature 1. Belongs to the ILVD / EDD family. Cofactor: binds 1 4FE-4S cluster (potential). P9WKJ5 No intermediary metabolism and respiration P65154 P9WKJ5 GO:0004160,GO:0009097,GO:0046872,GO:0009099,GO:0051539 4.2.1.9 P9WKJ5 NC_000962.3 Mycobrowser_v5 CDS 230899 231534 . + 0 Rv0195 Rv0195 Possibly sensor part of a two component regulatory system. Possible two component transcriptional regulatory protein (probably LuxR-family) Rv0195, (MTV033.03), len: 211 aa. Possible two-component response regulator, luxR family, similar to many e.g. U00008|ECOHU49_15 regulatory protein narP from Escherichia coli strain K12 (225 aa), FASTA scores: opt: 232, E(): 7.3e-09, (29.2% identity in 219 aa overlap). Start chosen by similarity. Contains probable helix-turn-helix motif at aa 166-187 (Score 1164, +3.15 SD). O53646 No regulatory proteins O53646 O53646 GO:0006355,GO:0043565,GO:0006350,GO:0003700,GO:0000156,GO:0000160,GO:0005622 O53646 NC_000962.3 Mycobrowser_v5 CDS 232231 234519 . + 0 Rv0197 Rv0197 Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0197, (MTV033.05), len: 762 aa. Possible oxidoreductase, similar to others e.g. 9948789|AAG06102.1|AE004699_7|B83307 probable molybdopterin oxidoreductase from Pseudomonas aeruginosa strain PAO1 (769 aa); 5441785|CAB46809.1|AL096811|T36812 probable dehydrogenase from Streptomyces coelicolor (747 aa), FASTA scores: opt: 617, E(): 9.8e-30, (29.9% identity in 762 aa overlap); BAB04334.1|AP001509 assimilatory nitrate reductase (catalytic subunit) from Bacillus halodurans (743 aa); etc. L0T2Z1 No intermediary metabolism and respiration O53648 L0T2Z1 GO:0030151,GO:0055114,GO:0016491,GO:0051536 1.-.-.- L0T2Z1 NC_000962.3 Mycobrowser_v5 CDS 454421 455386 . + 0 Rv0377 Rv0377 Possibly involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably LysR-family) Rv0377, (MTV036.12), len: 321 aa. Probable transcription regulator, lysR family, showing similarity with many hypothetical transcriptional regulators lysR homolog e.g. P32484|YEIE_ECOLI|M89774 hypothetical transcriptional regulator from Escherichia coli (293 aa), FASTA scores: opt: 265, E(): 4.9e-11, (28.6% identity in 266 aa overlap). Also similar to Rv2282c from Mycobacterium tuberculosis. Contains PS00044 bacterial regulatory protein lysR family signature. Seems to belong to the LysR family of transcriptional regulators. P9WMF7 No regulatory proteins O53712 P9WMF7 GO:0006350,GO:0003700,GO:0006355 P9WMF7 NC_000962.3 Mycobrowser_v5 CDS 238392 241292 . - 0 Rv0202c mmpL11 Unknown. Thought to be involved in fatty acid transport. Probable conserved transmembrane transport protein MmpL11 Rv0202c, (MTV033.10c), len: 966 aa. Probable mmpL11, conserved transmembrane transport protein (see citation below), equivalent to Z95398|MLCL622.16c from Mycobacterium leprae (1014 aa), FASTA scores: opt: 4076, E(): 0, (72.8% identity in 1017 aa overlap). Member of RND superfamily, similar to several putative transport proteins e.g. P96687 from Bacillus subtilis (724 aa), FASTA scores: opt: 594, E(): 9.1e-29, (26.9% identity in 717 aa overlap); etc. Belongs to the MmpL family. P9WJT9 No cell wall and cell processes P65374 P9WJT9 GO:0005886,GO:0016021 P9WJT9 NC_000962.3 Mycobrowser_v5 CDS 250120 251598 . + 0 Rv0210 Rv0210 Unknown Hypothetical protein Rv0210, (MTCY08D5.05), len: 492 aa. Hypothetical unknown protein. Possibly membrane protein; has hydrophobic stretches around aa 333 - 381. P96392 No conserved hypotheticals P96392 P96392 NC_000962.3 Mycobrowser_v5 CDS 251782 253602 . + 0 Rv0211 pckA Rate-limiting gluconeogenic enzyme [catalytic activity: GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2]. Probable iron-regulated phosphoenolpyruvate carboxykinase [GTP] PckA (phosphoenolpyruvate carboxylase) (PEPCK)(pep carboxykinase) Rv0211, (MTCY08D5.06), len: 606 aa. Probable pckA (alternate gene names: pckG and pck1), iron-regulated phosphoenolpyruvate carboxykinase [GTP], equivalent to Z95398|MLCL622_21 probable phosphoenolpyruvate carboxykinase from Mycobacterium leprae (609 aa), FASTA score: (86.1% identity in 605 aa overlap). Also highly similar to others e.g. PPCK_NEOFR|P22130 phosphoenolpyruvate carboxykinase [GTP] (608 aa), FASTA scores: opt: 2287, E(): 0, (55.9% identity in 598 aa overlap). Contains PS00505 Phosphoenolpyruvate carboxykinase (GTP) signature. Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. P9WIH3 No intermediary metabolism and respiration P65686 P9WIH3 GO:0005737,GO:0006094,GO:0046872,GO:0004613,GO:0005525 4.1.1.32 P9WIH3 NC_000962.3 Mycobrowser_v5 CDS 296005 296493 . + 0 Rv0245 Rv0245 Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0245, (MTV034.11), len: 162 aa. Possible oxidoreductase, equivalent to AL022486|MLCB1883_17|T44882 probable oxidoreductase from Mycobacterium leprae (162 aa), FASTA scores: opt: 860, E(): 0, (83.4% identity in 157 aa overlap). Also similar to several hypothetical proteins and various oxidoreductases e.g. AAK24246.1|AE005898 NADH:riboflavin 5'-phosphate oxidoreductase from Caulobacter crescentus (174 aa); Q02058|DIM6_STRCO|CAA45048.1 actinorhodin polyketide dimerase from streptomyces coelicolor (177 aa), FASTA scores: opt: 308, E(): 3. 2e-15, (37.8% identity in 143 aa overlap). Also similar to Z84498|Rv1939|MTCY09F9.25c from Mycobacterium tuberculosis (171 aa), FASTA scores: opt: 517, E(): 3.5e-30, (49.4% identity in 158 aa overlap). O53667 No intermediary metabolism and respiration O53667 O53667 GO:0055114,GO:0016491,GO:0010181 1.-.-.- O53667 NC_000962.3 Mycobrowser_v5 CDS 256064 257677 . + 0 Rv0214 fadD4 Function unknown, but involved in lipid degradation. Probable fatty-acid-CoA ligase FadD4 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv0214, (MTCY08D5.09), len: 537 aa. Probable fadD4, fatty-acid-CoA synthetase, similar to many e.g. 4CL_PINTA|P41636 4-coumarate--CoA ligase (537 aa), FASTA scores: opt: 622, E(): 1e-31, (30.0% identity in 514 aa overlap). Also similar to others from Mycobacterium tuberculosis e.g. MTCY6A4.14 FASTA score: (30.7% identity in 501 aa overlap); MTCY493_27, MTCY07A7_11, MTCI28_6. Contains PS00455 putative AMP-binding domain signature. P96396 No lipid metabolism P96396 P96396 GO:0008152,GO:0016874 6.2.1.- P96396 NC_000962.3 Mycobrowser_v5 CDS 262812 264023 . + 0 Rv0220 lipC Function unknown; lipolytic enzyme probably involved in cellular metabolism. Probable esterase LipC Rv0220, (MTCY08D5.15), len: 403 aa. Probable esterase, similar to others proteins and esterases from various organisms and Mycobacterium tuberculosis e.g. Q50681 (431 aa), FASTA scores: opt: 841, E(): 0, (38.2% identity in 408 aa overlap); Rv1426c, Rv1399c, etc. Contains PS00122 Carboxylesterases type-B serine active site. P96402 No intermediary metabolism and respiration P96402 P96402 GO:0016787 3.1.1.- P96402 NC_000962.3 Mycobrowser_v5 CDS 266301 267764 . - 0 Rv0223c Rv0223c Thought to oxidize a wide variety of aliphatic and aromatic aldehydes. Probable aldehyde dehydrogenase Rv0223c, (MTCY08D5.18), len: 487 aa. Probable aldehyde dehydrogenase, similar to others e.g. A75608|6460525|AAF12231.1|AE001862_57 aldehyde dehydrogenase from Deinococcus radiodurans strain R1 (495 aa); Q47943 L-sorbosone dehydrogenase NAD(P) dependent from Gluconobacter oxydans (498 aa), FASTA scores: opt: 1157, E (): 0, (42.1% identity in 482 aa overlap); etc. Also similar to Rv0768, Rv2858c, etc from Mycobacterium tuberculosis. Contains PS00687 Aldehyde dehydrogenases glutamic acid active site; and PS00070 Aldehyde dehydrogenases cysteine active site. Belongs to the aldehyde dehydrogenases family. I6X8S7 No intermediary metabolism and respiration 3B4W P96405 I6X8S7 GO:0016491,GO:0055114 1.2.1.- I6X8S7 NC_000962.3 Mycobrowser_v5 CDS 269834 271564 . - 0 Rv0226c Rv0226c Unknown Probable conserved transmembrane protein Rv0226c, (MTCY08D5.21c), len: 576 aa. Probable conserved transmembrane protein, equivalent, except in N-terminal part, to AC32114.1|AL583926 conserved membrane protein from Mycobacterium leprae (600 aa), FASTA scores: opt: 2086, E(): 0, (70.3% identity in 579 aa overlap). Also similar to AL021411|SC7H1_20 from Streptomyces coelicolor (483 aa), FASTA scores: opt: 180, E(): 0.00028, (26.5 identity in 388 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P96408 No cell wall and cell processes P96408 GO:0016021 P96408 NC_000962.3 Mycobrowser_v5 CDS 276058 277764 . + 0 Rv0231 fadE4 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE4 Rv0231, (MTCY08D5.27), len: 568 aa. Probable fadE4, acyl-CoA dehydrogenase, similar to many e.g. O29752 acyl-CoA dehydrogenase (ACD-3) from Archaeoglobus fulgidus (576 aa), FASTA scores: opt: 1788, E(): 0, (51.0% identity in 577 aa overlap); ACDB_BACSU|P45857 acyl-CoA dehydrogenase from Bacillus subtilis (379 aa), FASTA scores: opt: 232, E(): 2.2e- 08, (21.6% identity in 291 aa overlap). P96414 No lipid metabolism P96414 P96414 GO:0055114,GO:0003995 1.3.99.- P96414 NC_000962.3 Mycobrowser_v5 CDS 279605 281140 . - 0 Rv0234c gabD1 Involved in 4-aminobutyrate (GABA) degradation pathway [catalytic activity: succinate semialdehyde + NAD(P)(+) + H(2)O = succinate + NAD(P)H]. Succinate-semialdehyde dehydrogenase [NADP+] dependent (SSDH) GabD1 Rv0234c, (MTCY08D5.30c), len: 511 aa. gabD1, succinate-semialdehyde dehydrogenase [NADP+] dependent, equivalent to AL022486|MLCB1883_6 probable aldehyde dehydrogenase from Mycobacterium leprae (457 aa), FASTA scores: opt: 2617, E(): 0, (85.7% identity in 455 aa overlap). Also highly similar to Q55585|GABD|SLR0370 probable succinate-semialdehyde dehydrogenase from Synechocystis sp. strain PCC 6803 (454 aa), FASTA scores: opt: 1676, E(): 0, (55.8% identity in 455 aa overlap); and similar to others e.g. GABD_ECOLI|P25526 succinate-semialdehyde dehydrogenase from Escherichia coli (482 aa), FASTA scores: opt: 929, E(): 0, (36.5% identity in 452 aa overlap); etc. Note that similar to other cytosolic aldehyde dehydrogenases with EC number: 1.2.1.3. Also similar to Rv0768|aldA semialdehyde dehydrogenase from Mycobacterium tuberculosis (489 aa); and gabD2|Rv1731|MTCY04C12.16 possible succinate-semialdehyde dehydrogenase [NADP+] dependent from Mycobacterium tuberculosis (518 aa). Contains PS00070 aldehyde dehydrogenases cysteine active site. Belongs to the aldehyde dehydrogenases family. Could start at different site by homology. Note that previously known as gabD2. P9WNX9 No intermediary metabolism and respiration P71989 P9WNX9 GO:0009013,GO:0006099,GO:0055114 1.2.1.16 P9WNX9 NC_000962.3 Mycobrowser_v5 CDS 298116 298862 . - 0 Rv0247c Rv0247c Involved in interconversion of fumarate and succinate (aerobic respiration) [catalytic activity: succinate + acceptor = fumarate + reduced acceptor]. Probable succinate dehydrogenase [iron-sulfur subunit] (succinic dehydrogenase) Rv0247c, (MTV034.13c), len: 248 aa. Probable succinate dehydrogenase, iron-sulfur subunit, highly similar to CAC44313.1|AL596043 putative succinate dehydrogenase iron-sulfur subunit from Streptomyces coelicolor (259 aa); and similar to iron-sulphur protein subunits of fumarate reductase or succinate dehydrogenases from many bacteria e.g. NP_147618.1|7521083|B72691 fumarate reductase iron-sulfur protein from Aeropyrum pernix (305 aa); NP_069516.1|2649932|AAB90556.1|AE001057 succinate dehydrogenase iron-sulfur subunit B (sdhB) from Archaeoglobus fulgidus (236 aa); etc. Also similar to Q10761|FRDB_MYCTU|7431693|F70762 fumarate reductase iron-sulfur protein from Mycobacterium tuberculosis (247 aa), FASTA scores: opt: 358, E():1e-16, (31.3% identity in 214 aa overlap). Contains PS00197 2Fe-2S ferredoxins, iron-sulfur binding region signature. Note that succinate dehydrogenase forms generally part of an enzyme complex containing four subunits: a flavoprotein (Rv0248c ?), an iron-sulfur (Rv0247c ?), and two hydrophobic anchor proteins (Rv0249c ?). O53669 No intermediary metabolism and respiration O53669 O53669 GO:0009055,GO:0046872,GO:0055114,GO:0000104,GO:0006099,GO:0051537 1.3.99.1 O53669 NC_000962.3 Mycobrowser_v5 CDS 1547832 1549151 . + 0 Rv1375 Rv1375 Function unknown Conserved hypothetical protein Rv1375, (MTCY02B12.09), len: 439 aa. Conserved hypothetical protein, similar to hypothetical proteins from several organisms e.g. Q52871|U39409 Rhizobium leguminosarum (420 aa), FASTA scores: E(): 2e-30, (34.4% identity in 378 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). P9WF27 No conserved hypotheticals P71803 P9WF27 P9WF27 NC_000962.3 Mycobrowser_v5 CDS 287186 288352 . + 0 Rv0237 lpqI Unknown Probable conserved lipoprotein LpqI Rv0237, (MTV034.03), len: 388 aa. Probable lpQI, conserved lipoprotein, equivalent to AL022486|MLCB1883_8|T44873 probable secreted hydrolase from Mycobacterium leprae (387 aa), FASTA scores: opt: 1831, E(): 0, (73.3% identity in 390 aa overlap). Also similar to other lipoproteins and various hydrolases e.g. P40406|2126897|YBBD_BACSU|I39839 hypothetical 70.6 KDA lipoprotein from Bacillus subtilis (642 aa); P48823|HEXA_ALTSO beta-hexosaminidase a precursor from alteromonas SP. (598 aa), FASTA scores: opt: 415, E(): 5.8e-17, (31.2% identity in 343 aa overlap); PCC6803|P74340 beta-glucosidase from Synechocystis sp. (538 aa), FASTA scores: opt: 414, E(): 6.1e-17, (30.6 identity in 320 aa overlap). Contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. L7N6B0 No cell wall and cell processes Q7DA74 L7N6B0 GO:0004553,GO:0005975 L7N6B0 NC_000962.3 Mycobrowser_v5 CDS 290665 292029 . - 0 Rv0242c fabG4 Involved in the fatty acid biosynthesis pathway (first reduction step) [catalytic activity: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH]. Probable 3-oxoacyl-[acyl-carrier protein] reductase FabG4 (3-ketoacyl-acyl carrier protein reductase) Rv0242c, (MTV034.08c), len: 454 aa. Probable fabG4, 3-oxoacyl-[acyl-carrier protein] reductase, equivalent to 3063883|CAA18568.1|AL022486|MLCB1883_13|T44878 3-oxoacyl-[acyl-carrier protein] reductase homolog from Mycobacterium leprae (454 aa), FASTA scores: opt: 2486, E(): 0, (84.8% identity in 454 aa overlap). C-terminal part highly similar to many FabG proteins e.g. U39441|VHU3944 1_2 from Vibrio harveyi (244 aa), FASTA scores: opt: 562, E(): 3.4e-28, (40.2% identity in 241 aa overlap); U91631|PAU91631_3 from Pseudomonas aeruginosa (247 aa), FASTA scores: opt: 584, E(): 1.5e-29, (44.4% identity in 241 aa overlap). Has N-terminal extension of ~200 aa and C-terminal part contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. I6Y778 No lipid metabolism 3M1L,3Q6I O53665 I6Y778 GO:0055114,GO:0004316 1.1.1.100 I6Y778 NC_000962.3 Mycobrowser_v5 CDS 293798 295633 . - 0 Rv0244c fadE5 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE5 Rv0244c, (MTV034.10c), len: 611 aa. Probable fadE5, acyl-CoA dehydrogenase, equivalent to AL022486|MLCB1883_15 from Mycobacterium leprae (611 aa), FASTA scores: opt: 3598, E(): 0, (89.4% identity in 611 aa overlap). Also highly similar to AL0211|MTV007.14 from Mycobacterium tuberculosis (609 aa), FASTA scores: opt: 2576, E(): 0, (64.6% identity in 611 aa overlap); and to various other bacterial proteins described as putative acyl-CoA dehydrogenases e.g. AE0010|AE001025_6 from Archaeoglobus fulgidus (387 aa), FASTA scores: opt: 229, E(): 6.8e-08, (29.8% identity in 409 aa overlap); etc. O53666 No lipid metabolism O53666 O53666 GO:0003995,GO:0055114,GO:0050660 1.3.99.- O53666 NC_000962.3 Mycobrowser_v5 CDS 306374 307858 . - 0 Rv0255c cobQ1 Involved in cobalamin biosynthesis Probable cobyric acid synthase CobQ1 Rv0255c, (MTV034.21c), len: 494 aa. Probable cobQ1, cobyric acid synthase, similar to many e.g. Z46611|RCBLUGNS_8 cobyric acid synthase from R.capsulatus (483 aa), FASTA scores: opt: 1239, E(): 0, (47.1% identity in 493 aa overlap); P29932|COBQ_PSEDE cobyric acid synthase from Pseudomonas denitrificans (484 aa), FASTA scores: opt: 1168, E():0, (44.9% identity in 490 aa overlap); etc. Belongs to the COBB/COBQ family, COBQ subfamily. Note that previously known as cobQ. P9WP95 No intermediary metabolism and respiration P0A532 P9WP95 GO:0015420,GO:0006541,GO:0009236 P9WP95 NC_000962.3 Mycobrowser_v5 CDS 612255 612605 . + 0 Rv0520 Rv0520 Could cause methylation. Possible methyltransferase/methylase (fragment) Rv0520, (MTCY20G10.10), len: 116 aa. Possible fragment of methyltransferase (possibly first part), highly similar to part of several methyltransferases e.g. Q43445|U43683 S-adenosyl-L-methionine:DELTA24-sterol-C-methyltransferase from Glycine max (Soybean)(367 aa), FASTA scores: opt: 190, E(): 2.3e-12, (39.2% identity in 74 aa overlap). Also some similarity to MTCY19G5_5 from Mycobacterium tuberculosis. Possibly continues as Rv0521 but we can find no frameshift to account for this. O33365 No intermediary metabolism and respiration O33365 O33365 GO:0008168,GO:0008152 2.1.1.- O33365 NC_000962.3 Mycobrowser_v5 CDS 312759 314168 . - 0 Rv0261c narK3 Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate. Responsible for the translocation of the substrate across the membrane. Probable integral membrane nitrite extrusion protein NarK3 (nitrite facilitator) Rv0261c, (MTCY06A4.05c), len: 469 aa. Probable nirK3, nitrite extrusion protein, integral membrane protein possibly member of major facilitator superfamily (MFS), equivalent to AAB41700.1|U72744 nitrite extrusion protein from Mycobacterium fortuitum (471 aa); and 2342627|CAB11406.1|Z98741|T44908 nitrite extrusion protein homolog from Mycobacterium leprae (517 aa; longer in N-terminus). Also similar to other nitrite extrusion proteins e.g. NARK_ECOLI|P10903|B1223 nitrite extrusion protein 1 from Escherichia coli strain K12 (463 aa), FASTA scores: opt: 755, E(): 0, (35.0% identity in 466 aa overlap). Belongs to the nark/NASA family of transporters. P95218 No cell wall and cell processes P95218 P95218 GO:0055085 P95218 NC_000962.3 Mycobrowser_v5 CDS 316511 317503 . - 0 Rv0265c Rv0265c Thought to be involved in iron transport across the membrane (import). Probable periplasmic iron-transport lipoprotein Rv0265c, (MTCY06A4.09c), len: 330 aa. Probable iron-transport lipoprotein, most similar to T36412|5763945|CAB53324.1|AL109974 probable iron-siderophore binding lipoprotein from Streptomyces coelicolor (350 aa); and (N-terminus may be incorrect) to T14166|3560508|AAC82551.1|AF027770 fxuD protein from Mycobacterium smegmatis (420 aa), FASTA scores: opt: 385, E(): 1.5e-16, (32.3% identity in 232 aa overlap). Also similar to AAB97475.1|U02617 DtxR/iron regulated lipoprotein precursor from Corynebacterium diphtheriae (355 aa); FECB_ECOLI|P15028 iron(III) dicitrate-binding periplasmic protein (300 aa), FASTA scores: opt: 191, E(): 2.3e-05, (26.5% identity in 196 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Note that previously known as fecB2. L7N6B2 No cell wall and cell processes Q7DA51 L7N6B2 GO:0005381,GO:0006827 L7N6B2 NC_000962.3 Mycobrowser_v5 CDS 321331 322722 . + 0 Rv0267 narU Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate. Responsible for the translocation of the substrate across the membrane. Probable integral membrane nitrite extrusion protein NarU (nitrite facilitator) Rv0267, (MTCY06A4.11), len: 463 aa. Probable narU, nitrite extrusion protein, integral membrane protein possibly member of major facilitator superfamily (MFS), similar to other nitrite extrusion proteins e.g. NARU_ECOLI|P37758 nitrite extrusion protein 2 from Escherichia coli (462 aa), FASTA scores: opt: 630, E(): 4.4e-33, (38.9% identity in 463 aa overlap); and NARK_ECOLI|P10903|B1223 nitrite extrusion protein 1 from Escherichia coli strain K12 (463 aa), FASTA scores: opt: 607, E(): 1.3e-31, (42.0% identity in 457 aa overlap). Also similar to Rv0261c, Rv2329c, Rv1737c, and to MLCB22_25 from Mycobacterium leprae (517 aa), FASTA score: (35.1 identity in 459 aa overlap). Belongs to the nark/NASA family of transporters. P95224 No cell wall and cell processes P95224 P95224 GO:0015698,GO:0016021,GO:0055085,GO:0015103 P95224 NC_000962.3 Mycobrowser_v5 CDS 366150 372764 . - 0 Rv0304c PPE5 Function unknown PPE family protein PPE5 Rv0304c, (MTCY63.9c), len: 2204 aa. PPE5, Member of the Mycobacterium tuberculosis PE family (PPE, MPTR), similar to others e.g. Z95324|MTY13E10_16 from Mycobacterium tuberculosis (1443 aa), FASTA scores: E(): 0, (50.6% identity in 1403 aa overlap); Y04H_MYCTU|Q10778 from Mycobacterium tuberculosis (734 aa), FASTA scores: opt: 989, E(): 0, (42.3% identity in 522 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q6MX49 No PE/PPE Q6MX49 Q6MX49 Q6MX49 NC_000962.3 Mycobrowser_v5 CDS 330422 331003 . + 0 Rv0274 Rv0274 Function unknown Conserved protein Rv0274, (MTV035.02), len: 193 aa. Conserved protein, highly similar to AAK25058.1|AE005973 conserved hypothetical protein from Caulobacter crescentus (174 aa). Shows also some similarity to others hypothetical proteins e.g. AJ002571|BSAJ2571_7 from Bacillus subtilis (316 aa), FASTA scores: opt: 138, E(): 0.033, (27.1% identity in 133 aa overlap). Previous hits with Q56415|M85195 fosfomycin-resistance protein from serratia marcescens (141 aa), FASTA scores: opt: 82, E(): 1.1e -08, (29.1% identity in 151 aa overlap). Contains PS00082 Extradiol ring-cleavage dioxygenases signature near C-terminus. May belong to the vicinal-oxygen-chelate (VOC) superfamily of metalloenzymes (See Rawat et al., 2003). O53680 No conserved hypotheticals O53680 O53680 GO:0008198,GO:0055114,GO:0016702,GO:0019439 O53680 NC_000962.3 Mycobrowser_v5 CDS 333437 336310 . - 0 Rv0278c PE_PGRS3 Function unknown PE-PGRS family protein PE_PGRS3 Rv0278c, (MTV035.06c), len: 957 aa. PE_PGRS3, Member of the Mycobacterium tuberculosis PE family (see citation below), PGRS subfamily of gly-rich proteins, similar to many e.g. Z95890|MTCY28_25|Rv1759c from Mycobacterium tuberculosis (914 aa), FASTA scores: opt: 3849, E(): 0, (67.8% identity in 903 aa overlap). Contains PS00583 pfkB family of carbohydrate kinases signature 1. P9WIG3 No PE/PPE P56877 P9WIG3 P9WIG3 NC_000962.3 Mycobrowser_v5 CDS 340998 341906 . + 0 Rv0281 Rv0281 Possible methyltransferase Possible S-adenosylmethionine-dependent methyltransferase Rv0281, (MTV035.09), len: 302 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), member of Mycobacterium tuberculosis protein family that includes Rv0726c, Rv0731c, Rv3399, Rv1729c, etc. MTCY31_23 (325 aa), FASTA scores: opt: 1386, E(): 0, (69. 1% identity in 301 aa overlap). Contains possible N-terminal signal sequence. P9WFI9 No lipid metabolism O53686 P9WFI9 GO:0008168 2.1.1.- P9WFI9 NC_000962.3 Mycobrowser_v5 CDS 342130 344025 . + 0 Rv0282 eccA3 Function unknown ESX conserved component EccA3. ESX-3 type VII secretion system protein. Rv0282, (MTV035.10), len: 631 aa. eccA3, esx conserved component, ESX-3 type VII secretion system protein, similar to Y14967|MLCB628.18c hypothetical protein from Mycobacterium leprae (573 aa), FASTA scores: opt: 916, E(): 0, (38.7% identity in 568 aa overlap). Also similar to Mycobacterium tuberculosis proteins e.g. Z94121|MTY15F10.26 (619 aa), FASTA scores: opt: 743, E(): 0, (29.9% identity in 612 aa overlap). Member of CFXQ, CBXP family - 9 members in Mycobacterium tuberculosis. Contains PS00017 ATP/GTP-binding site motif A (P-loop). P9WPI3 No cell wall and cell processes O53687 P9WPI3 GO:0005737,GO:0017111,GO:0005524 P9WPI3 NC_000962.3 Mycobrowser_v5 CDS 364044 364469 . + 0 Rv0301 vapC2 Unknown Possible toxin VapC2 Rv0301, (MTCY63.06), len: 141 aa. Possible vapC2, toxin, part of toxin-antitoxin (TA) operon with Rv0300, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. Rv2757c, Rv0229c, Rv2546, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WFB9 No virulence, detoxification, adaptation 3H87 O07228 P9WFB9 P9WFB9 NC_000962.3 Mycobrowser_v5 CDS 372820 375711 . - 0 Rv0305c PPE6 Function unknown PPE family protein PPE6 Rv0305c, (MTCY63.10c), len: 963 aa. PPE6, Member of the Mycobacterium tuberculosis PE family (PPE, MPTR), similar to others e.g. Y04H_MYCTU|Q10778 from Mycobacterium tuberculosis (734 aa), FASTA scores: opt: 1340, E(): 0, (40.9% identity in 815 aa overlap). Q6MX48 No PE/PPE Q6MX48 Q6MX48 Q6MX48 NC_000962.3 Mycobrowser_v5 CDS 352149 353036 . + 0 Rv0289 espG3 Function unknown ESX-3 secretion-associated protein EspG3 Rv0289, (MTV035.17), len: 295 aa. EspG3, ESX-3 secretion-associated protein, equivalent to CAC32061.1|AL583926 possible DNA-binding protein from Mycobacterium leprae (289 aa); and showing some similarity to Rv3866|G70656|CAB06238.1|Z94121|MTCY15F10.23 from Mycobacterium tuberculosis (276 aa), FASTA scores: opt: 149, E(): 0.0035, (27.7% identity in 289 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WJC7 No cell wall and cell processes P9WJC7 P9WJC7 NC_000962.3 Mycobrowser_v5 CDS 364605 365237 . + 0 Rv0302 Rv0302 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably TetR/AcrR-family) Rv0302, (MTCY63.07), len: 210 aa. Probable transcription regulatory protein, TetR family (see citation below), with its N-terminus similar to N-terminus of several repressors and regulatory proteins of TetR/AcrR family e.g. ACRR_ECOLI|P34000 potential acraB operon repressor from Escherichia coli (215 aa), FASTA scores: opt: 172, E(): 3.1e-05, (22.7% identity in 194 aa overlap). Also similar in N-terminus to N-terminus of MTCY07A7.24 hypothetical regulator from Mycobacterium tuberculosis FASTA score: (38.7% identity in 62 aa overlap). Contains probable helix-turn helix motif from aa 35-56 (Score 1728, +5.07 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07229 No regulatory proteins O07229 O07229 GO:0006350,GO:0003700,GO:0006355 O07229 NC_000962.3 Mycobrowser_v5 CDS 354498 355883 . + 0 Rv0291 mycP3 Thought to have proteolytic activity. Probable membrane-anchored mycosin MycP3 (serine protease) (subtilisin-like protease) (subtilase-like) (mycosin-3) Rv0291, (MTV035.19), len: 461 aa. Probable mycP3, membrane-anchored serine protease (mycosin) (see Brown et al., 2000), similar to several others in mycobacteria e.g. Z94121|MTY15F10_28|Rv1796 from Mycobacterium tuberculosis (446 aa), FASTA scores: opt: 1168, E(): 0, (44.6% identity in 453 aa overlap); Rv3886c; Rv3883c; Rv3449; and Y14967|MLCB628_4|MLCB628.04 from Mycobacterium leprae (446 aa), FASTA scores: opt: 1159, E(): 0, (43.5 identity in 446 aa overlap). Has signal sequence and hydrophobic stretch at C-terminus, followed by short positively charged segment, that seems to act as a membrane anchor. Contains PS00137 Serine proteases, subtilase family, histidine active site signature. Belongs to peptidase family S8 (also known as the subtilase family), pyrolysin subfamily. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). O53695 No intermediary metabolism and respiration O53695 O53695 GO:0004252,GO:0006508 3.4.21.- O53695 NC_000962.3 Mycobrowser_v5 CDS 359758 361155 . - 0 Rv0296c Rv0296c Function unknown; probably involved in cellular metabolism. Probable sulfatase Rv0296c, (MTCY63.01c, MTV035.24c), len: 465 aa. Probable sulfatase, possibly an aryl-/steryl-sulfatase or a sulfamidase (sulfohydrolase) (sulphamidase). Similar to various hydrolases e.g. AAG41945.1|AF304053_1|AF304053 heparan N-sulfatase from Mus musculus (502 aa); NP_061292.1|6851181|AAF29460.1|AF153827_1|AF153827 N-sulfoglucosamine sulfohydrolase (sulfamidase) (sulphamidase) from Mus musculus (502 aa); AAG17206.1|AF217203_1|AF217203 heparan sulfate sulfamidase from Canis familiaris (507 aa); P08842|STS_HUMAN|1360652 steryl-sulfatase precursor (steroid sulfatase) (steryl-sulfate sulfohydrolase) (arylsulfatase C) (ASC) from Homo sapiens (583 aa); ARSB_FELCA|P33727 arylsulfatase B precursor (535 aa), FASTA scores: opt: 231, E(): 1.7e-08, (30.3% identity in 261 aa overlap). Also similarity with 4 others sulfatases in Mycobacterium tuberculosis. Contains sulfatases signature 1 (PS00523). Note that previously known as atsG. Q6MX51 No intermediary metabolism and respiration Q6MX51 Q6MX51 GO:0008484,GO:0008152 3.-.-.- Q6MX51 NC_000962.3 Mycobrowser_v5 CDS 361334 363109 . + 0 Rv0297 PE_PGRS5 Function unknown PE-PGRS family protein PE_PGRS5 Rv0297, (MTCY63.02), len: 591 aa. PE_PGRS5, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to others e.g. Y03A_MYCTU|Q10637 from Mycobacterium tuberculosis (603 aa), FASTA scores: opt: 1884, E(): 0, (53.7% identity in 635 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q6MX50 No PE/PPE Q6MX50 Q6MX50 Q6MX50 NC_000962.3 Mycobrowser_v5 CDS 385173 385943 . - 0 Rv0317c glpQ2 Glycerophosphoryl diester phosphodiesterase hydrolyzes deacylated phospholipids to G3P and the corresponding alcohols [catalytic activity: a glycerophosphodiester + H(2)O = an alcohol + SN-glycerol 3-phosphate]. Possible glycerophosphoryl diester phosphodiesterase GlpQ2 (glycerophosphodiester phosphodiesterase) Rv0317c, (MTCY63.22c), len: 256 aa (start uncertain, chosen by homology). Possible glpQ2, glycerophosphoryl diester phosphodiesterase, similar to others e.g. E75317|6459876|AAF11631.1|AE002044_4 glycerophosphoryl diester phosphodiesterase from Deinococcus radiodurans (285 aa); P10908|UGPQ_ECOLI from Escherichia coli (247 aa), FASTA scores: opt: 220, E(): 5.2e-07, (28.0% identity in 250 aa overlap). Also similar to MTCY01A6.27 from Mycobacterium tuberculosis FASTA score: (27.5% identity in 247 aa overlap). O07244 No intermediary metabolism and respiration O07244 O07244 GO:0008889,GO:0006629,GO:0006071 3.1.4.46 O07244 NC_000962.3 Mycobrowser_v5 CDS 391352 392032 . + 0 Rv0324 Rv0324 Possibly involved in transcriptional mechanism. Possible transcriptional regulatory protein (possibly ArsR-family) Rv0324, (MTCY63.29), len: 226 aa. Possible transcriptional regulator, arsR family, with its N-terminus similar to the N-terminus of other DNA-binding proteins e.g. P30346|MERR_STRLI probable mercury resistance operon from Streptomyces lividans (125 aa), FASTA scores: opt: 154, E(): 0.002, (32.2% identity in 90 aa overlap), and its C-terminal part similar to hypothetical bacterial proteins e.g. P54510|YQHL_BACSU hypothetical 14.6 kDa protein from Bacillus subtilis (126 aa), FASTA scores: opt: 159, E(): 0.00097, (35.5% identity in 76 aa overlap). Most similar to AJ005575|SPE005575_2 ORF1 required for antibiotic production from Streptomyces peucetius (226 aa), FASTA scores: opt: 816, E(): 0, (60.7% identity in 211 aa overlap). Also similar in C-terminus to MTCY164.26 molybdopterin biosynthesis moeb protein from Mycobacterium tuberculosis FASTA score: (36.8% identity in 114 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O08446 No regulatory proteins O08446 O08446 GO:0006355,GO:0008272,GO:0004792,GO:0006350,GO:0003700,GO:0005622 O08446 NC_000962.3 Mycobrowser_v5 CDS 396201 397367 . + 0 Rv0331 Rv0331 Function unknown; probably involved in cellular metabolism. Possible dehydrogenase/reductase Rv0331, (MTCY63.36), len: 388 aa. Possible dehydrogenase/reductase, similar to various dehydrogenases/reductases e.g. NP_103779.1|14022957|BAB49565.1|AP002999 flavoprotein reductase from Mesorhizobium loti (377 aa); NP_147681.1 predicted NAD(FAD)-dependent dehydrogenase from Aeropyrum pernix (381 aa); DHSU_CHRVI|Q06530 sulfide dehydrogenase (431 aa), FASTA scores: opt: 347, E(): 6.8e-15, (25.6% identity in 348 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07255 No intermediary metabolism and respiration O07255 O07255 GO:0016491,GO:0055114 1.-.-.- O07255 NC_000962.3 Mycobrowser_v5 CDS 398658 399524 . + 0 Rv0334 rmlA dTDP-L-rhamnose biosynthesis within the O antigen biosynthesis pathway of lipopolysaccharide biosynthesis [catalytic activity: dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-glucose]. Alpha-D-glucose-1-phosphate thymidylyltransferase RmlA (dTDP-glucose synthase) (dTDP-glucose pyrophosphorylase) Rv0334, (MTCY279.01), len: 288 aa. RmlA (alternate gene name: rfbA), alpha-D-glucose-1-phosphate thymidylyl-transferase (see citations below), equivalent to CAC32020.1|AL583925 glucose-1-phosphate thymidyltransferase from Mycobacterium leprae (288 aa). Also highly similar to others e.g. AAG29804.1|AF235050 glucose-1-phosphate thymidylyltransferase from Streptomyces rishiriensis (296 aa); RBA1_ECOLI|P37744 glucose-1-phosphate thymidylyltransferase from Escherichia coli strain K12 (293 aa), FASTA scores: opt: 1199, E(): 0, (62.0% identity in 284 aa overlap). Belongs to the glucose-1-phosphate thymidylyltransferase family. P9WH13 No intermediary metabolism and respiration P72017 P9WH13 GO:0008879,GO:0046872,GO:0045226 2.7.7.24 P9WH13 NC_000962.3 Mycobrowser_v5 CDS 403193 405841 . - 0 Rv0338c Rv0338c Function unknown; probably involved in cellular metabolism. Probable iron-sulfur-binding reductase Rv0338c, (MTCY279.05c), len: 882 aa. Probable iron-sulphur-binding reductase, possibly membrane-bound, equivalent to CAC32018.1|AL583925 probable iron-sulphur-binding reductase from Mycobacterium leprae (880 aa). Also highly similar to others e.g. T36608|5019323|CAB44376.1|AL078610 probable iron-sulfur-binding reductase from Streptomyces coelicolor (760 aa), FASTA scores: opt: 1658, E(): 0, (49.9% identity in 772 aa overlap); BAB07521.1|AP001520 iron-sulphur-binding reductase from Bacillus halodurans (700 aa). Contains PS00070 Aldehyde dehydrogenases cysteine active site and two of PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature. First of several possible start sites chosen. O33268 No intermediary metabolism and respiration O33268 O33268 GO:0051536,GO:0055114,GO:0016491,GO:0009055 1.-.-.- O33268 NC_000962.3 Mycobrowser_v5 CDS 405950 408448 . - 0 Rv0339c Rv0339c Could be involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0339c, (MTCY279.06c), len: 832 aa. Possible transcriptional regulator, showing very weak similarity with parts of others. Contains PS00017 ATP/GTP-binding site motif A (P-loop); and probable helix-turn helix motif from aa 778-799 (Score 1041, +2.73 SD). O33269 No regulatory proteins O33269 O33269 GO:0006355,GO:0043565,GO:0006350,GO:0003700,GO:0000156,GO:0000160,GO:0005622 O33269 NC_000962.3 Mycobrowser_v5 CDS 409362 410801 . + 0 Rv0341 iniB Unknown Isoniazid inductible gene protein IniB Rv0341, (MTCY13E10.01), len: 479 aa. IniB, isoniazid-inducible gene, (see citations below). Protein very Gly-, Ala-rich, similar to cell wall proteins e.g. P27483|GRP_ARATH glycine-rich cell wall structural protein from A.thaliana (338 aa), FASTA scores: opt: 532, E(): 5.2e-13, (39.3% identity in 321 aa overlap). MEME-mast analysis shows similarity to product of upstream gene, Rv0340. P9WJ97 No cell wall and cell processes P9WJ97 EMB,INH NC_000962.3 Mycobrowser_v5 CDS 412757 414238 . + 0 Rv0343 iniC Unknown Isoniazid inductible gene protein IniC Rv0343, (MTCY13E10.03), len: 493 aa. IniC, isoniazid-inducible gene, (see citations below). Shows slight similarity to P40983|YOR6_THER8 hypothetical protein (402 aa), FASTA scores: opt: 196, E(): 2.6e-05, (25.9% identity in 228 aa overlap). Also some similarity to upstream ORF Rv0342|iniA. Contains (PS00017) ATP/GTP-binding site motif A (P-loop). Note that the iniA gene is also induced by the antibiotic ethambutol, an agent that inhibits cell wall biosynthesis by a mechanism that is distinct from isoniazid. P9WJ95 No cell wall and cell processes O06294 P9WJ95 GO:0003924,GO:0005525 EMB,INH P9WJ95 NC_000962.3 Mycobrowser_v5 CDS 415502 416965 . - 0 Rv0346c ansP2 Thought to be involved in transport of L-asparagine across the membrane. Responsible for the translocation of the substrate across the membrane. Possible L-asparagine permease AnsP2 (L-asparagine transport protein) Rv0346c, (MTCY13E10.06c), len: 487 aa. Possible ansP2, L-asparagine permease, integral membrane protein belonging to family containing many amino acid permeases, highly similar to G467030|B2126_F2_85|NP_301937.1|NC_002677 probable L-asparagine permease from Mycobacterium leprae (498 aa); and NP_301938.1|NC_002677 probable L-asparagine permease from Mycobacterium leprae (505 aa). Also highly similar to others e.g. P77610|ANSP_ECOLI L-asparagine permease from Escherichia coli strain K-12 (499 aa). Also highly similar to ANSP1|Rv2127|MT2186|MTCY261_22|O33261 probable L-asparagine permease from Mycobacterium tuberculosis (489 aa), FASTA score: (72.1% identity in 473 aa overlap). And shows some similarity to MTCY3G12.14 from Mycobacterium tuberculosis. Belongs to the amino acid permease family (APC family). Note that previously known as aroP2. P9WQM7 No cell wall and cell processes P0A4W0 P9WQM7 GO:0006865,GO:0016021,GO:0005886,GO:0055085,GO:0015171 NC_000962.3 Mycobrowser_v5 CDS 422452 423639 . + 0 Rv0352 dnaJ1 Acts as a co-chaperone. Stimulates, jointly with GRPE|Rv0351, the ATPase activity of DNAK|Rv0350. Seems to be regulated negatively by HSPR (Rv0353 product). Probable chaperone protein DnaJ1 Rv0352, (MTCY13E10.12), len: 395 aa. Probable dnaJ1, chaperone protein, equivalent to AAA25363.1|M95576 DNA J heatshock protein from Mycobacterium leprae (389 aa). Also highly similar to others. Contains both DnaJ signatures (PS00636, and PS00637). Belongs to the DNAJ family. Cofactor: binds two zinc ions per monomer. Note that sequence differs from DNAJ_MYCTU|P07881 due to a frameshift at the N-terminus. Note that previously known as dnaJ. P9WNV9 No virulence, detoxification, adaptation P0A548 P9WNV9 GO:0006260,GO:0005737,GO:0031072,GO:0046872,GO:0006457,GO:0009408,GO:0051082,GO:0005524 P9WNV9 NC_000962.3 Mycobrowser_v5 CDS 435471 436769 . - 0 Rv0357c purA Involved in AMP biosynthesis (first committed step). Plays an important role in the de novo pathway of purine nucleotide biosynthesis [catalytic activity: GTP + imp + L-aspartate = GDP + phosphate + adenylosuccinate]. Probable adenylosuccinate synthetase PurA (imp--aspartate ligase) (ADSS) (ampsase) Rv0357c, (MTCY13E10.19c), len: 432 aa. Probable purA, adenylosuccinate synthase, equivalent to AL023514|MLCB4_13 from adenylosuccinate synthetase Mycobacterium leprae (432 aa), FASTA scores: opt: 2555, E(): 0, (87.9% identity in 431 aa overlap). Also highly similar to many bacterial adenylosuccinates synthetases e.g. P12283|PURA_ECOLI adenylosuccinates synthetase from Escherichia coli (431 aa), FASTA scores: E(): 0, (51.1% identity in 425 aa overlap); etc. Belongs to the adenylosuccinate synthetase family. P9WHN3 No intermediary metabolism and respiration P65880 P9WHN3 GO:0004019,GO:0005737,GO:0000287,GO:0006164,GO:0005525 6.3.4.4 P9WHN3 NC_000962.3 Mycobrowser_v5 CDS 453230 454372 . - 0 Rv0376c Rv0376c Function unknown Conserved hypothetical protein Rv0376c, (MTV036.11c), len: 380 aa. Conserved hypothetical protein, highly similar to T35481|4008539|CAA22508.1|AL034492|SC6C5.10 hypothetical protein from Streptomyces coelicolor (395 aa); and AAK64260.1|AF373840_20 ORF377 hypothetical CoxI from Arthrobacter nicotinovorans (377 aa). And similar to other conserved hypothetical proteins e.g. NP_101963.1|14021136|BAB47749.1|AP002994 hypothetical protein from Mesorhizobium loti (245 aa). Note that C-terminus shows similarity with C-termini of CAB76248.1|X82447|COXF CoxF protein from Pseudomonas/Oligotropha carboxidovorans (280 aa); CAB76250.1|X82447|COXI CoxI protein from Pseudomonas/Oligotropha carboxidovorans (330 aa); and AJ224684|BJAJ4684_6 cooxS from Bradyrhizobium japonicum (176 aa), FASTA scores: E(): 1.9e-17, (47.1% identity in 138 aa overlap). Also some partial similarity with AJ224684|BJAJ4684_5 cooxS from Bradyrhizobium japonicum (107 aa), FASTA scores: opt: 321, E(): 4.2e-14, (53.3% identity in 92 aa overlap); E1184330|Z99120|YURF YURF protein from Bacillus subtilis (330 aa), FASTA scores: opt: 170, E(): 2.9e- 16, (27.5% identity in 345 aa overlap). Also similar to downstream ORF Rv0372c from Mycobacterium tuberculosis (251 aa), FASTA scores: E(): 2.1e-06, (30.7% identity in 277 aa overlap). O53711 No conserved hypotheticals O53711 O53711 O53711 NC_000962.3 Mycobrowser_v5 CDS 439871 441253 . + 0 Rv0362 mgtE Thought to be involved in Mg2+ transport. Responsible for the translocation of the substrate across the membrane. Possible Mg2+ transport transmembrane protein MgtE Rv0362, (MTCY13E10.24), len: 460 aa. Possible mgtE, magnesium (Mg2+) transport transmembrane protein; C-terminal region is highly similar to MGTE|G780283 putative Mg2+ transporter from Providencia stuarti (314 aa), FASTA scores: E(): 0, (47.2% identity in 307 aa overlap) (N-terminus extends approx. 150 aa further upstream compared to P. stuarti ORF). Also similar in part to others e.g. AAK20879.1|AF334760_1|AF334760 putative Mg2+ transporter from Aeromonas hydrophila (455 aa); NP_231292.1|NC_002505 magnesium transporter from Vibrio cholerae (451 aa); NP_102305.1|NC_002678 Mg2+ transport protein from Mesorhizobium loti (454 aa); etc. Also similar to Rv1232c|MTV006.04c from Mycobacterium tuberculosis (435 aa). Extended hydrophobic segment spanning last 130 residues. Belongs to the MgtE family. O06312 No cell wall and cell processes O06312 O06312 GO:0006812,GO:0016021,GO:0008324 O06312 NC_000962.3 Mycobrowser_v5 CDS 444844 445233 . - 0 Rv0367c Rv0367c Unknown Hypothetical protein Rv0367c, (MTV036.02c), len: 129 aa. Hypothetical unknown protein. O53702 No conserved hypotheticals O53702 O53702 NC_000962.3 Mycobrowser_v5 CDS 445314 446525 . - 0 Rv0368c Rv0368c Function unknown Conserved hypothetical protein Rv0368c, (MTV036.03c), len: 403 aa. Conserved hypothetical protein, showing some similarity to AJ224684|BJAJ4684_4 cooxS protein from Bradyrhizobium japonicum (422 aa), FASTA scores: opt: 341, E(): 4.3e-13, (27.4% identity in 387 aa overlap); Rv2425c|MTCY428_22 hypothetical protein from Mycobacterium tuberculosis FASTA score: (30.7% identity in 238 aa overlap). Contains PS00213 Lipocalin signature. O53703 No conserved hypotheticals O53703 O53703 O53703 NC_000962.3 Mycobrowser_v5 CDS 448040 448633 . - 0 Rv0371c Rv0371c Function unknown Conserved hypothetical protein Rv0371c, (MTV036.06c), len: 197 aa. Conserved hypothetical protein, similar to other hypothetical proteins e.g. AL132824|SCAH10.09c|CAB60163.1|AL132824 hypothetical protein from Streptomyces coelicolor (207 aa), FASTA scores: opt: 247, E(): 4.5e-09, (32.3% identity in 195 aa overlap). Also weak similarity with YURE|D70017|Z99120|BSUB0017_134 hypothetical protein yurE from Bacillus subtilis (197 aa), FASTA scores: opt: 217, E(): 2.5e-08, (27.0% identity in 174 aa overlap). I6WY86 No conserved hypotheticals 2WE9,2WEE,2YES I6WY86 I6WY86 NC_000962.3 Mycobrowser_v5 CDS 448630 449385 . - 0 Rv0372c Rv0372c Function unknown Conserved hypothetical protein Rv0372c, (MTV036.07c), len: 251 aa. Conserved hypothetical protein, showing some similarity with CAB76248.1|X82447|COXF CoxF protein from Pseudomonas/Oligotropha carboxidovorans (280 aa); AJ224684|BJAJ4684_6 cooxS from Bradyrhizobium japonicum (176 aa), FASTA scores: opt: 186, E(): 1.6e-05, (41.1% identity in 95 aa overlap). Also similar to upstream ORF Rv0376c from Mycobacterium tuberculosis (380 aa), FASTA scores: E(): 6.8e-07, (31.0% identity in 277 aa overlap). O53707 No conserved hypotheticals O53707 O53707 O53707 NC_000962.3 Mycobrowser_v5 CDS 449404 451803 . - 0 Rv0373c Rv0373c Function unknown; probably involved in cellular metabolism [catalytic activity: CO + H(2)O + acceptor = CO(2) + reduced acceptor]. Probable carbon monoxyde dehydrogenase (large chain) Rv0373c, (MTV036.08c), len: 799 aa. Probable carbon monoxide dehydrogenase, large chain, highly similar to others e.g. AAD00363.1| U80806|CUTL carbon monoxide dehydrogenase large subunit CutL protein from Hydrogenophaga pseudoflava (803 aa); S49124|509391|CAA54902.1|X77931|1094915|2107180C|CUTA carbon-monoxide dehydrogenase large chain (cut operon) from Pseudomonas thermocarboxydovorans (842 aa); C56279|809566|CAA57829.1|X82447|OCCOXMSL4_3|COXL carbon-monoxide dehydrogenase large chain (cluster coxMSL) from Pseudomonas/Oligotropha carboxydovorans (809 aa), FASTA scores: opt: 2484, E(): 0, (56.0% identity in 804 aa overlap); etc. O53708 No intermediary metabolism and respiration O53708 O53708 GO:0005507,GO:0030151,GO:0055114,GO:0018492 1.2.99.2 O53708 NC_000962.3 Mycobrowser_v5 CDS 612598 612903 . + 0 Rv0521 Rv0521 Could cause methylation Possible methyltransferase/methylase (fragment) Rv0521, (replaces MTCY20G10.11), len: 101 aa. Possible fragment of methyltransferase (possibly second part), highly similar to C-terminus of several methyltransferases e.g. AAF87203.1|AF216282 sarcosine-dimethylglycine methyltransferase from Halorhodospira halochloris (279 aa). Possibly continuation of Rv0520 but we can find no frameshift to account for this. L7N6C0 No intermediary metabolism and respiration L7N6C0 GO:0008168 2.1.1.- NC_000962.3 Mycobrowser_v5 CDS 457841 458380 . - 0 Rv0382c pyrE Involved in pyrimidine biosynthesis (at the fifth step) [catalytic activity: orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate]. Probable orotate phosphoribosyltransferase PyrE (OPRT) (oprtase) Rv0382c, (MTV036.17c), len: 179 aa. Probable pyrE, orotate phosphoribosyltransferase, equivalent to CAC32004.1|AL583925 probable purine/pyrimidine phosphoribosyltransferase from Mycobacterium leprae (179 aa). Also highly similar to many others e.g. T36540|4753874|CAB42037.1|AL049754|SCH10.28c probable orotate phosphoribosyltransferase from Streptomyces coelicolor (182 aa); H69115|2622996|AAB86326.1|AE000938_10|MTH1860 probable orotate phosphoribosyltransferase from Methanobacterium thermoautotrophicum (180 aa), FASTA scores: opt: 389, E(): 2.7e-20, (40.7% identity in 172 aa overlap); O08359|PYRE_SULAC|2065444|CAA73352.1|Y12822 orotate phosphoribosyltransferase from Sulfolobus acidocaldarius (197 aa); etc. Note that also similar to other puridine 5'-monophosphate synthases (umpA genes; UMP synthases), generally in N-terminus that corresponds to orotate phosphoribosyltransferase activity. Contains PS00589 PTS HPR component serine phosphorylation site signature. Belongs to the purine/pyrimidine phosphoribosyltransferase family. Note that previously known as umpA. Nucleotide position 458282 in the genome sequence has been corrected, A:G resulting in Y33Y. P9WHK9 No intermediary metabolism and respiration P0A5U0 P9WHK9 GO:0004588,GO:0006221,GO:0009116 2.4.2.10 P9WHK9 NC_000962.3 Mycobrowser_v5 CDS 459456 462002 . - 0 Rv0384c clpB Thought to be an ATPase subunit of an intracellular ATP-dependent protease. Seems to be regulated positively by sigh (Rv3223c product) and negatively by HSPR (Rv0353 product). Probable endopeptidase ATP binding protein (chain B) ClpB (ClpB protein) (heat shock protein F84.1) Rv0384c, (MTV036.19c), len: 848 aa. Probable clpB (alternate gene name: htpM), endopeptidase ATP-binding protein, chain B, equivalent to AC32007.1|AL583925 heat shock protein from Mycobacterium leprae (848 aa). Also highly similar to others e.g. P53532|CLPB_CORGL|1163118|AAB49540.1|U43536|CGU43536_1 CLPB protein (heat-inducible expression) from Corynebacterium glutamicum (852 aa), FASTA scores: opt: 4113, E(): 0, (74.5% identity in 846 aa overlap); T36551|4753885|CAB42048.1|AL049754|clpB|SCOEDB|SCH10.39c probable ATP-dependent proteinase ATP-binding chain from Streptomyces coelicolor (853 aa); P03815|CLPB_ECOLI|1788943|AAC75641.1|AE000345 CLPB protein (heat shock protein F84.1) from Escherichia coli strains K12 and O157:H7 (857 aa); etc. Also similar to Rv3596c|ClpC from Mycobacterium tuberculosis. Contains PS00870 and PS00871 Chaperonins clpA/B signatures and two PS000017 ATP/GTP-binding site motives a (P-loop). Belongs to the CLPA/CLPB family. Contains probable coiled-coil domain from aa 411-503. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P9WPD1 No virulence, detoxification, adaptation P63288 P9WPD1 GO:0005737,GO:0017111,GO:0005515,GO:0019538,GO:0006950,GO:0005524 P9WPD1 NC_000962.3 Mycobrowser_v5 CDS 468335 469594 . + 0 Rv0389 purT Involved in third step (first of two transformylation reactions) in de novo purine biosynthesis. This is an alternative enzyme to the PURN|Rv0956 gar transformylase (5'-phosphoribosylglycinamide formyltransferase). Catalyzes two reactions: the first one is the production of beta-formyl glycinamide ribonucleotide (gar) from formate, ATP and beta gar; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [catalytic activity: formate + ATP + 5'-phospho-ribosylglycinamide = 5'-phosphoribosyl-N-formylglycinamide + ADP + pyrophosphate]. Probable phosphoribosylglycinamide formyltransferase 2 PurT (GART 2) (gar transformylase 2) (5'-phosphoribosylglycinamide transformylase 2) (formate-dependent gar transformylase) Rv0389, (MTCY04D9.01, MTV036.24), len: 419 aa. Probable purT, phosphoribosylglycinamide formyltransferase 2, similar to others e.g. P33221|PURT_ECOLI|B1849 phosphoribosylglycinamide formyltransferase 2 from Escherichia coli strain K-12 (391 aa), FASTA scores: opt: 481, E(): 1.3e-22, (40.1% identity in 379 aa overlap); etc. Belongs to the PurK / PurT family. Cofactor: magnesium. P95197 No intermediary metabolism and respiration P95197 P95197 GO:0016742,GO:0000287,GO:0009152,GO:0005524 2.1.2.- P95197 NC_000962.3 Mycobrowser_v5 CDS 483977 485734 . + 0 Rv0404 fadD30 Function unknown, but involved in lipid degradation. Fatty-acid-AMP ligase FadD30 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) Rv0404, (MTCY04D9.17-MTCY22G10.00), len: 585 aa. fadD30, fatty-acid-AMP synthetase, similar to many e.g. MBU75685_1|AAB52538.1|U75685 acyl-CoA synthase from Mycobacterium bovis (582 aa); MASC_MYCLE|P54200 masc protein from Mycobacterium leprae (372 aa), FASTA scores: opt: 888, E(): 0, (44.2% identity in 342 aa overlap). Also similar to Y06J_MYCTU|Q10976 hypothetical 67.9 kDa protein (626 aa), FASTA scores: opt: 1463, E(): 0, (42.4% identity in 568 aa overlap). P9WQ57 No lipid metabolism P95213 P9WQ57 GO:0008152,GO:0016874 6.2.1.- P9WQ57 NC_000962.3 Mycobrowser_v5 CDS 472781 474106 . + 0 Rv0393 Rv0393 Function unknown Conserved 13E12 repeat family protein Rv0393, (MTCY04D9.05), len: 441 aa. Member of Mycobacterium tuberculosis 13E12 repeat family of conserved proteins, similar to many e.g. Rv1148c, Rv1945, Rv3467, Rv0336|MTCY279_3 (503 aa), FASTA scores: E(): 0, (61.1% identity in 347 aa overlap). P95201 No insertion seqs and phages P95201 P95201 NC_000962.3 Mycobrowser_v5 tRNA 924110 924183 . + 0 MTB000012 aspT - tRNA-Asp (GTC) aspT, tRNA-Asp, anticodon gtc, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 1025321 1025393 . - 0 MTB000014 argT - tRNA-Arg (CCT) argT, tRNA-Arg, anticodon cct, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 478566 479753 . - 0 Rv0400c fadE7 Function unknown, but involved in lipid degradation. Acyl-CoA dehydrogenase FadE7 Rv0400c, (MTCY04D9.12c), len: 395 aa. Probable fadE7, acyl-CoA dehydrogenase, similar to many e.g. CAC12923.1|AL445403 putative acyl CoA dehydrogenase from Streptomyces coelicolor (397 aa); G624219 glutaryl-CoA dehydrogenase precursor (438 aa), FASTA scores: opt: 1161, E(): 0, (48.1% identity in 391 aa overlap); etc. P95208 No lipid metabolism P95208 P95208 GO:0003995,GO:0055114,GO:0050660 1.3.99.- NC_000962.3 Mycobrowser_v5 CDS 1896475 1896867 . + 0 Rv1671 Rv1671 Unknown Probable membrane protein Rv1671, (MTV047.06), len: 130 aa. Probable membrane protein. Weak similarity to mercuric transport proteins. O53918 No cell wall and cell processes O53918 O53918 NC_000962.3 Mycobrowser_v5 CDS 490783 491793 . + 0 Rv0407 fgd1 Catalyzes oxidation of glucose-6-phosphate to 6-phosphogluconolactone using coenzyme F420 (an *-hydroxy-5-deazaflavin derivative) as the electron acceptor. F420-dependent glucose-6-phosphate dehydrogenase Fgd1 Rv0407, (MTCY22G10.03), len: 336 aa. fgd1, F420-dependent glucose-6-phosphate dehydrogenase, equivalent to others from Mycobacteria e.g. AAD38165.1|AF152394_3 from Mycobacterium avium (336 aa), FASTA scores: opt: 2082, E(): 0, (89.9% identity in 336 aa overlap); AL023514|MLCB 4_7 from Mycobacterium leprae (336 aa), FASTA scores: opt: 2069, E(): 0, (89.0% identity in 336 aa overlap). Also similar to other dehydrogenases e.g. CAA77276.1|Y18730 F420-dependent alcohol dehydrogenase from Methanofollis liminatans (330 aa). Also similar to many proteins from Mycobacterium tuberculosis e.g. Rv0953c, Rv0791c, etc. Note that previously known as fgd. P9WNE1 No intermediary metabolism and respiration 3B4Y,3C8N P96253 P9WNE1 GO:0016491,GO:0055114 1.-.-.- P9WNE1 NC_000962.3 Mycobrowser_v5 CDS 491786 493858 . + 0 Rv0408 pta Involved at the last step (of two) in the conversion of acetate to acetyl-CoA [catalytic activity: acetyl-CoA + phosphate = CoA + acetyl phosphate]. Probable phosphate acetyltransferase Pta (phosphotransacetylase) Rv0408, (MTCY22G10.04), len: 690 aa. Probable pta, phosphate acetyltransferase, highly similar to others e.g. PTA_ECOLI|P39184|11279789|JX0357|B2297 phosphate acetyltransferase from Escherichia coli strain K12 (713 aa), FASTA scores: opt: 1303, E(): 0, (38.0% identity in 718 aa overlap); etc. Belongs to the phosphate acetyltransferase and butyryltransferase family. P9WHP1 No intermediary metabolism and respiration P96254 P9WHP1 GO:0008152,GO:0008959,GO:0005737 2.3.1.8 P9WHP1 NC_000962.3 Mycobrowser_v5 CDS 499713 500366 . + 0 Rv0413 mutT3 Possibly involved in the go system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite DA and DC residues of template DNA with almost equal efficiency thus leading to A.T to G.C transversions. MutT specifically degrades 8-oxo-dGTP to the monophosphate [catalytic activity: 8-oxo-dGTP + H2O = 8-oxo-dGMP + pyrophosphate]. Possible mutator protein MutT3 (7,8-dihydro-8-oxoguanine-triphosphatase) (8-oxo-dGTPase) (dGTP pyrophosphohydrolase) Rv0413, (MTCY22G10.10), len: 217 aa. Possible mutT3, mutator protein (see citation below), showing some similarity with e.g. MUTT_PROVU|P32090 mutator mutt protein from Proteus vulgaris (112 aa), FASTA scores: opt: 151, E(): 0.0008, (40.7% identity in 59 aa overlap). Seems to belong to the NUDIX hydrolase family. P9WIX9 No information pathways P96259 P9WIX9 GO:0006260,GO:0016787,GO:0046872,GO:0006281 3.6.1.- P9WIX9 NC_000962.3 Mycobrowser_v5 CDS 500350 501018 . - 0 Rv0414c thiE Involved in thiamine biosynthesis. Condenses 4-methyl-5-(beta-hydroxyethyl)-thiazole monophosphate (THZ-P) and 4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-pp) to form thiamine monophosphate (TMP) [catalytic activity: 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-4-methyl-5-(2-phosphonooxyethyl)-thiazole = diphosphate + thiamine monophosphate]. Thiamine-phosphate pyrophosphorylase ThiE (TMP pyrophosphorylase) (TMP-PPASE) (thiamine-phosphate synthase) Rv0414c, (MTCY22G10.11c), len: 222 aa. thiE, thiamin phosphate pyrophosphorylase, equivalent to Q9ZBL5|AL035159|MLCB1450_17 probable thiamine-phosphate pyrophosphorylase from Mycobacterium leprae (235 aa), FASTA scores: opt: 1095, E(): 0, (78.0% identity in 223 aa overlap). Also similar to others e.g. T34974|5689976|CAB52013.1|AL109663 probable thiamin phosphate pyrophosphorylase from Streptomyces coelicolor (223 aa); THIE_ECOLI|P30137 thie protein from Escherichia coli strain K12 (211 aa), FASTA scores: opt: 275, E(): 7.8e-12, (37.8% identity in 196 aa overlap); etc. Belongs to the TMP-PPASE family. P9WG75 No intermediary metabolism and respiration 3O63 P66916 P9WG75 GO:0009228,GO:0004789,GO:0000287 2.5.1.3 P9WG75 NC_000962.3 Mycobrowser_v5 CDS 2584486 2584755 . + 0 Rv2312 Rv2312 Unknown Hypothetical protein Rv2312, (MTCY3G12.22c), len: 89 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLB9 No conserved hypotheticals P9WLB9 NC_000962.3 Mycobrowser_v5 CDS 502366 503124 . + 0 Rv0417 thiG Involved in thiamine biosynthesis. Required for the synthesis of the thiazole moiety of thiamine. Probable thiamin biosynthesis protein ThiG (thiazole biosynthesis protein) Rv0417, (MTCY22G10.14), len: 252 aa. Probable thiG, thiamin biosynthesis protein, equivalent to AL035159|MLCB1450_20|T44741|THIG probable thiamin biosynthesis protein from Mycobacterium leprae (261 aa), FASTA scores: opt: 1380, E(): 0, (86.8% identity in 250 aa overlap). Also highly similar to others e.g. SCOEDB|SC6E10.03|T35490|THIG probable thiazole biosynthesis protein from Streptomyces coelicolor (264 aa); F82761|9105679|AAF83593.1|AE003919_4|XF0783|THIG thiamin biosynthesis protein thiG from Xylella fastidiosa (275 aa); P30139|THIG_ECOLI|7448315|B65206|409790|AAC43089.1|U00006 THIG protein thiamin biosynthesis protein from Escherichia coli strain K-12 (281 aa); etc. Belongs to the THIG family. P9WG73 No intermediary metabolism and respiration P96263 P9WG73 GO:0016829,GO:0009228,GO:0005737 4.-.-.- P9WG73 NC_000962.3 Mycobrowser_v5 CDS 503496 504998 . + 0 Rv0418 lpqL Function unknown; hydrolyzes peptides and/or proteins. Probable lipoprotein aminopeptidase LpqL Rv0418, (MTCCY22G10.15), len: 500 aa. Probable lpqL, lipoprotein aminopeptidase, similar to others e.g. B83278|9949035|AAG06327.1|AE004720_3|AE004720|PA2939 probable aminopeptidase from Pseudomonas aeruginosa (536 aa); P80561|APX_STRGR|SGAP|S66427 aminopeptidase from Streptomyces griseus (284 aa) (homology only with C-terminus of Rv0418); P37302|APE3_YEAST|1077010|A54134 aminopeptidase Y from Saccharomyces cerevisiae (537 aa); etc. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. P96264 No cell wall and cell processes P96264 P96264 GO:0006508,GO:0004177 3.4.11.- P96264 NC_000962.3 Mycobrowser_v5 CDS 508582 510225 . - 0 Rv0423c thiC Involved in thiamine biosynthesis. Required for the synthesis of the hydromethylpyrimidine (HMP) moiety of thiamine (4-amino-2-methyl-5-hydroxymethylpyrimidine). Probable thiamine biosynthesis protein ThiC Rv0423c, (MTCY22G10.20c), len: 547 aa. Probable thiC, thiamin biosynthesis protein, equivalent to Q9ZBL0|THIC_MYCLE|11279601|T44743|AL035159|MLCB1450_22 thiamine biosynthesis protein from Mycobacterium leprae (547 aa), FASTA scores: opt: 3283, E(): 0, (90.1% identity in 547 aa overlap). Also highly similar to others e.g. P45740|THIC_BACSU thiamin biosynthesis protein from Bacillus subtilis (590 aa), FASTA scores: opt: 2295, E(): 0, (65.2% identity in 580 aa overlap); P30136|THIC_ECOLI THIC protein from Escherichia coli strain K12 (631 aa), FASTA scores: opt: 2141, E(): 0, (62.1% identity in 568 aa overlap); etc. Belongs to the ThiC family. P9WG79 No intermediary metabolism and respiration P66911 P9WG79 GO:0016829,GO:0046872,GO:0009228,GO:0051539 4.-.-.- P9WG79 NC_000962.3 Mycobrowser_v5 CDS 510702 515321 . - 0 Rv0425c ctpH Metal cation-transporting ATPase; possibly catalyzes the transport of an undetermined metal cation with the hydrolysis of ATP [catalytic activity: ATP + H(2)O + undetermined metal cation(in) = ADP + phosphate + undetermined metal cation(out)]. Possible metal cation transporting P-type ATPase CtpH Rv0425c, (MTCY22G10.22c), len: 1539 aa. Possible ctpH, metal cation-transporting P-type ATPase (transmembrane protein), showing some similarity with CAA17934.1|AL022118|13093871|CAC32203.1|AL583926 putative cation-transporting ATPase from Mycobacterium leprae (1609 aa). Also similar to others ATPases e.g. AE000873_1 cation-transporting P-ATPase from Methanobacterium thermoautotrop (844 aa), FASTA score: (30.5% identity in 827 aa overlap); AB69720.1|AL137166 putative transport ATPase from Streptomyces coelicolor (1472 aa); etc. C-terminal region similar to other ATPases from Mycobacterium tuberculosis e.g. Y05Q_MYCTU|Q10900 putative cation-transporting ATPase C (855 aa), FASTA scores: opt: 770, E(): 5.3e-32, (44.9% identity in 820 aa overlap). Nucleotide position 511518 in the genome sequence has been corrected, T:G resulting in I1268I. P96271 No cell wall and cell processes P96271 P96271 GO:0006754,GO:0016021,GO:0015693,GO:0015444,GO:0005524 3.6.1.- P96271 NC_000962.3 Mycobrowser_v5 CDS 516017 516892 . - 0 Rv0427c xthA Involved in base excision repair. Apurinic-apyrimidinic endonuclease. Supposed to remove the damaged DNA at cytosines and guanines by cleaving at the 3' side of the AP site by a beta-elimination reaction. Possibly exhibites 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase and ribonuclease H activities [catalytic activity: degradation of double-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing 5'-phosphomononucleotides]. Probable exodeoxyribonuclease III protein XthA (exonuclease III) (EXO III) (AP endonuclease VI) Rv0427c, (MTCY22G10.24c), len: 291 aa. Probable xthA (alternate gene name: xth), exodeoxyribonuclease III protein (see citation below), similar to others e.g. EX3_ECOLI|P09030 exodeoxyribonuclease III from Escherichia Coli strain K12 (268 aa), FASTA scores: opt: 360, E(): 1.2e-17, (29.3% identity in 270 aa overlap); etc. Belongs to the AP/EXOA family of DNA repair enzymes. P96273 No information pathways P96273 P96273 GO:0006281,GO:0004519,GO:0008853,GO:0005622,GO:0003677 3.1.11.2 P96273 NC_000962.3 Mycobrowser_v5 CDS 710782 711009 . + 0 Rv0616A vapB29 Unknown Possible antitoxin VapB29 Rv0616A, len: 75 aa. Possible vapB29, antitoxin, part of toxin-antitoxin (TA) operon with Rv0617, see Arcus et al. 2005. Similar to many others in M. tuberculosis e.g. Rv2530A (74 aa) 35.9% identity in 78 aa overlap P9WJ37 No virulence, detoxification, adaptation P9WJ37 NC_000962.3 Mycobrowser_v5 CDS 516895 517803 . - 0 Rv0428c Rv0428c Acetylation, substrate unknown GCN5-related N-acetyltransferase Rv0428c, (MTCY22G10.25c), len: 302 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain in C-terminal part. See Vetting et al. 2005. P96274 No intermediary metabolism and respiration P96274 NC_000962.3 Mycobrowser_v5 CDS 520324 521454 . + 0 Rv0433 Rv0433 Function unknown Conserved hypothetical protein Rv0433, (MTCY22G10.30), len: 376 aa. Conserved hypothetical protein, similar to other hypothetical proteins e.g. P77213|YBDK_ECOLI hypothetical 41.7 KD protein from Escherichia coli strain K12 (372 aa), FASTA scores: opt: 555, E(): 2e-30, (28.2% identity in 365 aa overlap). P9WPK9 No conserved hypotheticals P64693 P9WPK9 GO:0004357,GO:0006750,GO:0005524 6.3.-.- P9WPK9 NC_000962.3 Mycobrowser_v5 CDS 521514 522167 . + 0 Rv0434 Rv0434 Function unknown Conserved hypothetical protein Rv0434, (MTCY22G10.31), len: 217 aa. Conserved hypothetical protein, similar to AE002052_2 from Deinococcus radiodurans (213 aa), FASTA scores: opt: 258, E(): 4e-10, (31.9% identity in 213 aa overlap); SYCSLRB_122|Q55701 hypothetical 24.5 kDa protein from Synechocystis (214 aa), FASTA scores: opt: 156, E(): 0.00041, (28.4% identity in 204 aa overlap); MXABSGA_1|LON2_MYXXA|P36774 ATP-dependent protease la 2 from Myxococcus xanthus (826 aa), FASTA scores: opt: 160, E(): 0.00068, (28.4% identity in 197 aa overlap); etc. P96280 No conserved hypotheticals P96280 P96280 GO:0006508,GO:0004176 P96280 NC_000962.3 Mycobrowser_v5 CDS 522347 524533 . - 0 Rv0435c Rv0435c ATPase; possibly catalyzes the transport of undetermined substrate with hydrolyse of ATP [catalytic activity: ATP + H(2)O + undetermined substrate(in) = ADP + phosphate + undetermined substrate(out)]. Putative conserved ATPase Rv0435c, (MTCY22G10.32c), len: 728 aa. Putative conserved ATPase, similar to others e.g. SAV_SULAC|Q07590 sav protein involved in cell division from sulfolobus acidocaldarius (780 aa), FASTA scores: opt: 897, E(): 0, (34.5% identity in 693 aa overlap); NP_148637.1|7435761|B72479 transitional endoplasmic reticulum ATPase from Aeropyrum pernix (699 aa); etc. Also similar to Rv3610c and Rv2115c from Mycobacterium tuberculosis. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00674 AAA-protein family signature. P96281 No cell wall and cell processes P96281 P96281 GO:0051301,GO:0017111,GO:0005524 3.6.1.- P96281 NC_000962.3 Mycobrowser_v5 CDS 525387 526082 . - 0 Rv0437c psd Function unknown, but involved in lipid metabolism [catalytic activity: phosphatidyl-L-serine = phosphatidylethanolamine + CO(2)]. Possible phosphatidylserine decarboxylase Psd (PS decarboxylase) Rv0437c, (MTV037.01c), len: 231 aa (start uncertain). Possible psd, phosphatidylserine decarboxylase , equivalent to CAC29819.1|AL583918 conserved hypothetical protein from Mycobacterium leprae (243 aa); and highly similar to MLCB1450.11|T44729|4154044|CAA22695.1|AL035159 hypothetical protein from Mycobacterium leprae (202 aa), FASTA score: (74.6% identity in 197 aa overlap). Also similar to other phosphatidylserine decarboxylases e.g. NP_108058.1|14027249|BAB54203.1|AP003012 phosphatidylserine decarboxylase from Mesorhizobium loti (232 aa); AAK86872|g15156090|AGR_C_1963 phosphatidylserine decarboxylase from Agrobacterium tumefaciens (244 aa); AAG12422.1|AY005137|Psd phosphatidylserine decarboxylase from Chlorobium tepidum (216 aa); etc. P9WHQ5 No lipid metabolism P67547 P9WHQ5 GO:0006646,GO:0004609,GO:0048037 4.1.1.65 P9WHQ5 NC_000962.3 Mycobrowser_v5 CDS 527379 528314 . - 0 Rv0439c Rv0439c Function unknown; possibly involved in cellular metabolism. Probable dehydrogenase/reductase Rv0439c, (MTV037.03c), len: 311 aa. Probable dehydrogenase/reductase, equivalent to AL035159|MLCB1450_6|T44727 probable oxidoreductase from Mycobacterium leprae (304 aa), FASTA scores: opt: 1360, E(): 0, (69.2% identity in 302 aa overlap). Also highly similar to various oxidoreductases, generally dehydrogenases/reductases e.g. PA5031|C83017|9951320|AAG08416.1|AE004916_5|AE004916 probable short chain dehydrogenase from Pseudomonas aeruginosa (309 aa); Q03326|OXIR_STRAT probable oxidoreductase from Streptomyces antibioticus (298 aa), FASTA scores: opt: 400, E(): 1.2e-18, (34.6% identity in 298 aa overlap); etc. O53726 No intermediary metabolism and respiration O53726 O53726 GO:0055114,GO:0016491 1.-.-.- O53726 NC_000962.3 Mycobrowser_v5 CDS 528608 530230 . + 0 Rv0440 groEL2 Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions. 60 kDa chaperonin 2 GroEL2 (protein CPN60-2) (GroEL protein 2) (65 kDa antigen) (heat shock protein 65) (cell wall protein A) (antigen A) Rv0440, (MTV037.04), len: 540 aa. GroEL2 (alternate gene names: groL2, groEL-2, hsp65, hsp60), 60 kDa chaperonin 2 (see Shinnick 1987). Purified 65 kDa antigen can elicit a strong delayed-type hypersensitivity reaction in experimental animals infected with M. tuberculosis. This protein is one of the major immunoreactive proteins of the mycobacteria. This antigen contains epitopes that are common to various species of mycobacteria. Contains PS00296 Chaperonins cpn60 signature. Belongs to the chaperonin (HSP60) family. Phosphorylated in vitro by PknJ|Rv2088 (See Arora et al., 2010). P9WPE7 No virulence, detoxification, adaptation 1SJP,3RTK P0A520 P9WPE7 GO:0005737,GO:0042026,GO:0051082,GO:0005524 P9WPE7 NC_000962.3 Mycobrowser_v5 CDS 533833 534396 . - 0 Rv0445c sigK Involved in transcription mechanism. The sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released. Alternative RNA polymerase sigma factor SigK Rv0445c, (MTV037.09c), len: 187 aa. sigK, alternative RNA polymerase sigma factor (see citations below), highly similar to others e.g. 5531433|CAB50938.1|AL096849|T36745 probable RNA polymerase sigma factor from Streptomyces coelicolor (185 aa); NP_105607.1|14024791|BAB51393.1|AP003005 RNA polymerase sigma factor from Mesorhizobium loti (179 aa); 1654108|AAB17906.1|U11283|A58883 probable transcription initiation factor sigma E from Rhodobacter phaeroides (168 aa), FASTA scores: opt: 299, E(): 2e-14, (32.7% identity in 168 aa overlap); Q45585|SIGW_BACSU RNA polymerase sigma factor SIGW from Bacillus subtilis (187 aa), FASTA scores: opt: 213, E(): 2.9e-08, (26.8% identity in 179 aa overlap); etc. P9WGH7 No information pathways O53730 P9WGH7 GO:0016987,GO:0003700,GO:0006352,GO:0006355 P9WGH7 NC_000962.3 Mycobrowser_v5 CDS 535224 536507 . - 0 Rv0447c ufaA1 Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge [catalytic activity: S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid]. Probable cyclopropane-fatty-acyl-phospholipid synthase UfaA1 (cyclopropane fatty acid synthase) (CFA synthase) Rv0447c, (MTV037.11c), len: 427 aa (start uncertain). Probable ufaA1, cyclopropane-fatty-acyl-phospholipid synthase, similar to others e.g. NP_102178.1|14021351|BAB47964.1|AP002994 cyclopropane-fatty-acyl-phospholipid synthase from Mesorhizobium loti (378 aa); B82240|9655593|AAF94281.1|AE004192 cyclopropane-fatty-acyl-phospholipid synthase from Vibrio cholerae (432 aa); P30010|CFA_ECOLI cyclopropane-fatty-acyl-phospholipid synthase from Escherichia coli strain K-12 (382 aa); X55704|PPLPD_3 LPD-3 from P.putida (394 aa), FASTA scores: opt: 556, E(): 2.8e-30, (33.3% identity in 387 aa overlap); AE0005|HPAE000557_9 from Helicobacter pylori (389 aa), FASTA scores: opt: 539, E(): 3.9e-29, (34.3% identity in 382 aa overlap). O53732 No lipid metabolism O53732 O53732 GO:0008610,GO:0008825 2.1.1.79 O53732 NC_000962.3 Mycobrowser_v5 CDS 630040 631686 . + 0 Rv0538 Rv0538 Unknown Possible conserved membrane protein Rv0538, (MTCY25D10.17), len: 548 aa. Possible conserved membrane protein. Middle region highly similar to AAB63811.1|AF009829|MBE4863a|O32850 unknown protein from Mycobacterium bovis (295 aa) possible transmembrane protein with a repetitive proline, threonine-rich region at C-terminus. O06404 No cell wall and cell processes O06404 O06404 NC_000962.3 Mycobrowser_v5 CDS 538588 541491 . - 0 Rv0450c mmpL4 Unknown. Thought to be involved in fatty acid transport. Probable conserved transmembrane transport protein MmpL4 Rv0450c, (MTV037.14c), len: 967 aa. Probable mmpL4, conserved transmembrane transport protein (see citations below), member of RND superfamily, equivalent to U1740V|P54881|YV34_MYCLE hypothetical 105.2 kDa protein from Mycobacterium leprae (959 aa), FASTA scores: opt: 5051, E(): 0, (78.4% identity in 962 aa overlap). Also highly similar to other proteins from Mycobacterium tuberculosis e.g. Z83860|MTCY98.08 (962 aa), FASTA scores: opt: 3917, E(): 0, (61.3% identity in 950 aa overlap), MTCY20G9.34, etc. Contains PS00211 ABC transporters family signature. Belongs to the MmpL family. P9WJV3 No cell wall and cell processes O53735 P9WJV3 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 545375 545821 . - 0 Rv0455c Rv0455c Function unknown Conserved protein Rv0455c, (MTV037.19c), len: 148 aa. Conserved protein, equivalent to CAC31896.1|AL583925 possible secreted protein from Mycobacterium leprae (153 aa). Has hydrophobic stretch at N-terminus. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). O53740 No conserved hypotheticals O53740 O53740 NC_000962.3 Mycobrowser_v5 CDS 554882 556306 . - 0 Rv0465c Rv0465c Possibly involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv0465c, (MTV038.09c), len: 474 aa. Probable transcriptional regulator, highly similar to AC44331.1|AL596102 putative DNA-binding protein from Streptomyces coelicolor (489 aa); and similar to several hypothetical proteins and others transcriptional regulators. Some similarity in N-terminal region (1-100 aa) with repressors e.g. P06153|RPC_BPPH1 immunity repressor protein (144 aa), FASTA scores: opt: 130, E(): 0.084,(27.0% identity in 100 aa overlap). Very similar to Rv1129c|Z95585|MTCY22G8.18c from Mycobacterium tuberculosis (486 aa), FASTA scores: opt: 1475, E(): 0, (47.4% identity in 468 aa overlap). Contains probable helix-turn-helix motif at aa 19-40 (1827, +5.41 SD). P9WMI1 No regulatory proteins O53750 P9WMI1 GO:0043565,GO:0006350,GO:0045449 P9WMI1 NC_000962.3 Mycobrowser_v5 CDS 557527 558813 . + 0 Rv0467 icl1 Involved in glyoxylate bypass (at the first step), an alternative to the tricarboxylic acid cycle (in bacteria, plants, and fungi) [catalytic activity: isocitrate = succinate + glyoxylate]. Involved in the persistence in the host. Isocitrate lyase Icl (isocitrase) (isocitratase) Rv0467, (MTV038.11), len: 428 aa. Icl1, isocitrate lyase (see citations below), highly similar to many, closest to Z29367|RFISCILY_1 from R. fascians (429 aa), FASTA scores: opt: 2359, E(): 0, (80.7% identity in 429 aa overlap). Belongs to the isocitrate lyase family. Has 2-methyl-isocitrate lyase (MCL) activity in M. tuberculosis Erdman (See Munoz-Elias et al., 2006; Gould et al., 2006). Predicted possible vaccine candidate (See Zvi et al., 2008). P9WKK7 No intermediary metabolism and respiration 1F61,1F8I,1F8M P0A5H3 P9WKK7 GO:0006097,GO:0004451,GO:0006099,GO:0005737 4.1.3.1 P9WKK7 NC_000962.3 Mycobrowser_v5 CDS 560848 561711 . - 0 Rv0470c pcaA Involved in the mycolic acid modification or synthesis; essential for the cyclopropanation function. Required for cording and mycolic acid cyclopropane ring synthesis in the cell wall. Mycolic acid synthase PcaA (cyclopropane synthase) Rv0470c, (MTV038.14), len: 287 aa. PcaA (previously known as umaA2), mycolic acid synthase (cyclopropane synthase) (see citations below), equivalent to CAC31976.1|AL583925 Mycolic acid synthase from Mycobacterium leprae (295 aa); and highly similar to S72886|B2168_F3_130|467038|AAA17222.1|U00018 hypothetical protein from Mycobacterium leprae (308 aa); Q11195|CFA1_MYCTU cyclopropane-fatty-acyl-phospholipid synthase 1 (cyclopropane mycolic acid synthase 1) (287 aa) (see Glickman et al., 2000); U27357|MTU27357_1 cyclopropane mycolic acid synthase from Mycobacterium tuberculosis (287 aa), FASTA scores: opt: 1415, E(): 0, (72.8% identity in 287 aa overlap); and related enzymes e.g. MTCY20H10.25c|Z92772|MTY20H10_26 (287 aa), FASTA scores: opt: 1387, E(): 0, (72.5% identity in 287 aa overlap). P9WPB3 No lipid metabolism 1L1E Q7D9R5 P9WPB3 GO:0008610,GO:0008825 2.-.-.- P9WPB3 NC_000962.3 Mycobrowser_v5 CDS 562723 563427 . - 0 Rv0472c Rv0472c Possibly involved in transcriptional mechanism. Probable transcriptional regulatory protein (possibly TetR-family) Rv0472c, (MTV038.16c), len: 234 aa. Probable regulatory protein, possibly TetR family, equivalent to CAC31974.1|AL583925 possible TetR-family transcriptional regulator from Mycobacterium leprae (233 aa). Also similar to CAC01492.1|AL391017 putative transcriptional regulatory protein from Streptomyces coelicolor (218 aa); and CAC01371.1|AL390975 putative TetR-family transcriptional regulator from Streptomyces coelicolor (228 aa). Also similar to AL0212|MTV012_65 from Mycobacterium tuberculosis (246 aa), FASTA scores: opt: 327, E(): 1.8e-15, (31.0% identity in 232 aa overlap); and Z95120|MTCY07D11.18c (228 aa), FASTA scores: opt: 190, E(): 4.4e-06, (23.1% identity in 186 aa overlap). Contains probable helix-turn-helix doimain at aa 45-66 (Score 1429, +4.05 SD). P9WMD9 No regulatory proteins O53757 P9WMD9 GO:0006350,GO:0003700,GO:0006355 P9WMD9 NC_000962.3 Mycobrowser_v5 CDS 565797 566396 . + 0 Rv0475 hbhA Required for extrapulmonary dissemination. Mediates adherence to epithelial cells by binding to sulfated glycoconjugates present at the surface of these cells; binds heparin, dextran sulfate, fucoidan and chondroitin sulfate. Promotes hemagglutination of erythrocytes of certain host species. Induces mycobacterial aggregation. Iron-regulated heparin binding hemagglutinin HbhA (adhesin) Rv0475, hbhA (MTCY20G9.01), len: 199 aa. HbhA, iron-regulated heparin-binding hemagglutinin (see citations below), equivalent to CAC31971.1|AL583925 possible hemagglutinin from Mycobacterium leprae (188 aa). Contains possible N-terminal signal sequence and K-a-rich region at C-terminus: subcellular location: surface associated. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WIP9 No cell wall and cell processes P9WIP9 GO:0009986,GO:0008201,GO:0009405,GO:0007155 P9WIP9 NC_000962.3 Mycobrowser_v5 CDS 568964 569806 . - 0 Rv0480c Rv0480c Function unknown; hydrolytic enzyme probably involved in cellular metabolism. Possible amidohydrolase Rv0480c, (MTCY20G9.06c), len: 280 aa. Possible amidohydrolase, highly similar to NP_302587.1|NC_002677|CAC31966.1|AL583925 putative hydrolase from Mycobacterium leprae (271 aa). Also similar to other hydrolases and hypothetical proteins e.g. NP_601985.1|NC_003450 Predicted amidohydrolase from Corynebacterium glutamicum (266 aa); NP_459623.1|NC_003197 putative hydrolase from Salmonella typhimurium LT2 (262 aa); AL096822|SCGD3_8|NP_627996.1|NC_003888 probable hydrolase from Streptomyces coelicolor (264 aa), FASTA scores: opt: 368, E(): 6.1e-15, (34.2% identity in 272 aa overlap); YAUB_SCHPO|Q10166 hypothetical 35.7 kDa protein c26a3.11 from S. pombe (322 aa), FASTA scores: opt: 338, E():1.4e-13, (30.3% identity in 277 aa overlap); etc. Start changed since first submission (-60 aa). P9WJ01 No intermediary metabolism and respiration Q11146 P9WJ01 GO:0006807,GO:0016810 3.5.-.- P9WJ01 NC_000962.3 Mycobrowser_v5 CDS 570539 571648 . + 0 Rv0482 murB Involved in cell wall formation; peptidoglycan biosynthesis [catalytic activity: UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH]. Probable UDP-N-acetylenolpyruvoylglucosamine reductase MurB (UDP-N-acetylmuramate dehydrogenase) Rv0482, (MTCY20G9.08), len: 369 aa. Probable murB, UDP-N-acetylenolpyruvoylglucosamine reductase (see citation below), equivalent to CAC31964.1|AL583925 UDP-N-acetylenolpyruvoylglucosamine reductase from Mycobacterium leprae (367 aa). Also highly similar to others e.g. MURB_ECOLI|P08373 UDP-N-acetylenolpyruvoylglucosamine reductase from Escherichia coli (342 aa), FASTA scores: opt: 292, E(): 6.3e-12, (33.5% identity in 355 aa overlap); etc. Belongs to the MurB family. Cofactor: FAD. P9WJL9 No cell wall and cell processes P65460 P9WJL9 GO:0008762,GO:0007049,GO:0051301,GO:0007047,GO:0005737,GO:0055114,GO:0009252,GO:0008360,GO:0050660 1.1.1.158 P9WJL9 NC_000962.3 Mycobrowser_v5 CDS 575348 576790 . + 0 Rv0486 mshA Involved in the first step of mycothiol biosynthesis Glycosyltransferase MshA Rv0486, (MTCY20G9.12), len: 480 aa. MshA, glycosyltransferase (see citations below), highly similar to P54138|Y486_MYCLE|ML2443 possible glycosyl transferase from Mycobacterium leprae (428 aa); and S72892|B2168_C2_201 probable hexosyltransferase from Mycobacterium leprae (409 aa), FASTA scores: opt: 2375, E(): 0, (86.4% identity in 413 aa overlap). Also highly similar to CAC04040.1|AL391406 putative transferase from Streptomyces coelicolor (496 aa); and similar to various transferases e.g. NP_437172.1|NC_003078 putative membrane-anchored glycosyltransferase protein from Sinorhizobium meliloti (416 aa); O26550|U67601_1 LPS biosynthesis related protein from Methanococcus jannaschii (411 aa), FASTA score: (25.3% identity in 387 aa overlap); etc. Also similar to CAC87824.1|AJ316594 putative sucrose-phosphate synthase from Nostoc punctiforme (422 aa). Contains a match to Pfam entry PF00534 glycosyl_transf_1 - Glycosyl transferases group 1. P9WMY7 No intermediary metabolism and respiration P64707 P9WMY7 GO:0016757,GO:0009058 2.4.-.- P9WMY7 NC_000962.3 Mycobrowser_v5 CDS 577664 578269 . + 0 Rv0488 Rv0488 Unknown; possibly involved in transport of lysine across the membrane. Probable conserved integral membrane protein Rv0488, (MTCY20G9.14), len: 201 aa. Probable conserved integral membrane protein, LysE family possibly involved in transport of Lysine, similar to others and conserved hypothetical proteins e.g. AB93746.1|AL357613 putative membrane transport protein from Streptomyces coelicolor (204 aa); D83100|PA4365 probable transporter from Pseudomonas aeruginosa (200 aa); YGGA_ECOLI|P11667 hypothetical 21.7 kDa protein from Escherichia coli (197 aa), FASTA scores: opt: 382, E(): 1.1e-19, (39.1% identity in 179 aa overlap); CGLYSEG_2 C|P94633 lysine exporter protein (236 aa), FASTA scores: E(): 2.3e-07, (33.3% identity in 219 aa overlap). Also similar to Rv1986 from Mycobacterium tuberculosis. P9WK33 No cell wall and cell processes P64711 P9WK33 GO:0016021,GO:0005886,GO:0006865 NC_000962.3 Mycobrowser_v5 CDS 579349 580581 . + 0 Rv0490 senX3 Sensor part of a two component regulatory system. Probably forms part of a two-component regulatory system SENX3/REGX3; phosphorylates REGX3. Putative two component sensor histidine kinase SenX3 Rv0490, (MTCY20G9.16), len: 410 aa. Putative senX3, two-component sensor histidine kinase, transmembrane protein (see citations below), equivalent to O07129|SEX3_MYCBO sensor-like histidine kinase SENX3 from Mycobacterium bovis BCG (410 aa), FASTA scores: E(): 0, (99.5% identity in 410 aa overlap); and highly similar to P54883|SEX3_MYCLE|SENX3 sensor-like histidine kinase from Mycobacterium leprae (443 aa), FASTA score: (83.8% identity in 408 aa overlap). Also highly similar, except in N-terminus, to CAC31957.1|AL583925 probable two-component system sensor histidine kinase from Mycobacterium leprae (441 aa). Also highly similar to sensor kinase proteins from other organisms e.g. CAB77323.1|AL160331 putative sensor kinase protein from Streptomyces coelicolor (426 aa). P9WGK5 No regulatory proteins P0A600 P9WGK5 GO:0016021,GO:0018106,GO:0005886,GO:0007165,GO:0000155,GO:0000160,GO:0005524 2.7.13.3 P9WGK5 NC_000962.3 Mycobrowser_v5 CDS 581489 583378 . - 0 Rv0492c Rv0492c Function unknown; probably involved in cellular metabolism. Probable oxidoreductase GMC-type Rv0492c, (MT0511/MT0512, MTCY20G9.18c), len: 629 aa. Probable oxidoreductase GMC type, similar to others except in N-terminus e.g. P55582|AE000087_5|Y4NJ_RHISN hypothetical GMC-type oxidoreductase from Rhizobium sp. (505 aa), FASTA scores: opt: 873, E():0, (34.3% identity in 502 aa overlap); YTH2_RHOER|P46371 hypothetical 53.0 kDa GMC-type oxidoreductase from Rhodococcus erythropolis (493 aa), FASTA score: (25.7% identity in 521 aa overlap); YTH2_RHOSO|P46371 hypothetical 53.0 kDa gmc-type oxidoreductase from Rhodococcus erythropolis (493 aa), FASTA score: (25.7% identity in 521 aa overlap); NP_085596.1|NC_002679 probable oxidoreductase from Mesorhizobium loti (507 aa); NP_285451.1|NC_001264 GMC oxidoreductase from Deinococcus radiodurans (722 aa); NP_249055.1|NC_002516 probable oxidoreductase from Pseudomonas aeruginosa (531 aa); etc. Contains PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature, and PS00624 GMC oxidoreductases signature 2. Belongs to the GMC oxidoreductases family. Cofactor: FAD (by similarity). Note that start changed since first submission (previously 684 aa). P9WMV7 No intermediary metabolism and respiration Q11157 P9WMV7 GO:0055114,GO:0016614,GO:0050660 1.1.-.- P9WMV7 NC_000962.3 Mycobrowser_v5 CDS 591111 591347 . + 0 Rv0500A Rv0500A Function unknown Conserved protein Rv0500A, len: 78 aa. Conserved protein, similar to proteins from Mycobacterium leprae and Streptomyces coelicolor e.g. U00018_25 from Mycobacterium leprae cosmid B2168 (86 aa), FASTA scores: opt: 428, E(): 1.3e-27, (82.6% identity in 86 aa overlap); AL079345|SCE68_26 from Streptomyces coelicolor cosmid E6 (70 aa), FASTA scores: opt: 252, E(): 1.2 e-13, (72.2 identity in 54 aa overlap). P9WKT7 No conserved hypotheticals P9WKT7 GO:0003677 NC_000962.3 Mycobrowser_v5 CDS 591654 592784 . + 0 Rv0501 galE2 Involved in galactose metabolism [catalytic activity: UDP-glucose = UDP-galactose]. Possible UDP-glucose 4-epimerase GalE2 (galactowaldenase) (UDP-galactose 4-epimerase) (uridine diphosphate galactose 4-epimerase) (uridine diphospho-galactose 4-epimerase) Rv0501, (MTCY20G9.28), len: 376 aa. Possible galE2, UDP-glucose 4-epimerase, highly similar (except in N-terminus) to CAC31944.1|AL583925 possible glucose epimerase/dehydratase from Mycobacterium leprae (364 aa). N-terminus highly similar to S72923|B2168_C1_174|467075|AAA17259.1|U00018 hypothetical protein from Mycobacterium leprae (180 aa), FASTA scores: opt: 934, E(): 0, (89.6% identity in 164 aa overlap); and C-terminus highly similar to S72898|467050|AAA17234.1|U00018 hypothetical protein from Mycobacterium leprae (168 aa), FASTA scores: opt: 928, E(): 0, (82.7% identity in 168 aa overlap). Also highly similar to T36274|5123671|CAB45360.1|AL079345 probable epimerase from Streptomyces coelicolor (353 aa); and similar in part to other epimerases e.g. GALE_ECOLI|P09147 UDP-glucose 4-epimerase from Escherichia coli (338 aa), FASTA scores: opt: 241, E(): 6.7e-09, (28.2% identity in 294 aa overlap); etc. Belongs to the sugar epimerase family. Cofactor: NAD. Note that previously known as galE1. P9WKT3 No intermediary metabolism and respiration P0A5D1 P9WKT3 GO:0044237,GO:0050662,GO:0003824 P9WKT3 NC_000962.3 Mycobrowser_v5 CDS 593871 594779 . - 0 Rv0503c cmaA2 Essential for the cyclopropanation function. Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge. Mycolic acids, which represent the major constituent of mycobacterial cell wall complex, act as substrates [catalytic activity: S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid]. Cyclopropane-fatty-acyl-phospholipid synthase 2 CmaA2 (cyclopropane fatty acid synthase) (CFA synthase) (cyclopropane mycolic acid synthase 2) (mycolic acid trans-cyclopropane synthetase) Rv0503c, (MTCY20G9.30c), len: 302 aa. CmaA2 (alternate gene name: cma2), cyclopropane-fatty-acyl-phospholipid synthase 2 (mycolic acid trans-cyclopropane synthetase) (see citations below). Note that this protein has 302 aa and not 322 aa: we have chosen a different initiation codon on the basis of homology. Equivalent to S72886|B2168_F3_130 hypothetical protein from Mycobacterium leprae (308 aa), FASTA score: (78.9% identity in 303 aa overlap); and highly similar to other proteins from Mycobacterium leprae. Also similar to other proteins from Mycobacterium tuberculosis and Mycobacterium bovis BCG e.g. MTV038_14|UMAA2|Rv0470c|MTV038.14 putative mycolic acid synthesis/modification protein (287 aa) (57.2% identity in 297 aa overlap). P9WPB5 No lipid metabolism 1KPI,3HEM P0A5P0 P9WPB5 GO:0005737,GO:0008610,GO:0008825 2.1.1.79 P9WPB5 NC_000962.3 Mycobrowser_v5 CDS 595464 596585 . - 0 Rv0505c serB1 Removes a phosphate from phosphoserine [catalytic activity: phosphoserine + H2O = serine + phosphate]. Possible phosphoserine phosphatase SerB1 (PSP) (O-phosphoserine phosphohydrolase) (pspase) Rv0505c, (MTCY20G9.32c), len: 373 aa. Possible serB1, phosphoserine phosphatase, equivalent (but longer ~70 aa in N-terminus) to S72914|serB phosphoserine phosphatase from Mycobacterium leprae (300 aa), FASTA scores: opt: 1570, E(): 0, (83.0% identity in 306 aa overlap). C-terminus highly similar to CAB55344.1|AJ010584 phosphoserine phosphatase from Streptomyces coelicolor (266 aa). Low similarity to SERB_ECOLI|P06862 phosphoserine phosphatase from Escherichia coli strains K12 and O157:H7 (322 aa), FASTA scores: opt: 148, E(): 0.043, (24.0% identity in 150 aa overlap). C-terminus is also similar to O33611|AB004855_1|IMD_STRCN protein involved in inhibition of morphological differentiation from Streptomyces cyaneus (277 aa), FASTA score: (37.7% identity in 252 aa overlap). Seems to belong to the SERB family. Note that previously known as serB. P9WGJ3 No intermediary metabolism and respiration P66801 P9WGJ3 GO:0016791,GO:0008152 P9WGJ3 NC_000962.3 Mycobrowser_v5 CDS 613038 614342 . + 0 Rv0522 gabP Involved in 4-aminobutyrate (GABA) degradation pathway. Transporter for GABA. Responsible for the translocation of the substrate across the membrane. Probable GABA permease GabP (4-amino butyrate transport carrier) (GAMA-aminobutyrate permease) Rv0522, (MTCY20G10.12), len: 434 aa. Probable gabP, GABA permease (gamma-aminobutyrate permease), integral membrane protein, highly similar to others e.g. GABP_ECOLI|P25527 gaba permease from Escherichia coli (466 aa), FASTA scores: opt: 1218, E(): 0, (44.3% identity in 424 aa overlap); etc. Also similar to other M. tuberculosis permeases e.g. MTCY13E10.06c FASTA score: (34.4% identity in 407 aa overlap). Contains PS00218 Amino acid permeases signature. Overlaps and extends Rv0523c|MTCY25D10.01 from overlapping cosmid. Belongs to the amino acid permease family (APC family). L7N6B9 No cell wall and cell processes Q8VKK1 L7N6B9 GO:0006865,GO:0016021,GO:0055085,GO:0015171 L7N6B9 NC_000962.3 Mycobrowser_v5 CDS 3004035 3004748 . - 0 Rv2687c Rv2687c Thought to be involved in active transport of unidentified antibiotic across the membrane (export): antibiotic resistance by an export mechanism. Responsible for the translocation of the substrate across the membrane. Antibiotic-transport integral membrane leucine and valine rich protein ABC transporter Rv2687c, (MTCY05A6.08c), len: 237 aa. Antibiotic-transport integral membrane leu-, val-rich protein ABC transporter (see citation below), showing some similarity with two other hypothetical proteins, BAB59668|TVG0517148 from Thermoplasma volcanium (241 aa), FASTA scores: opt: 136, E(): 0.32, (23.1% identity in 208 aa overlap); and Q97U55|SSO3168 from Sulfolobus solfataricus (249 aa), FASTA scores: opt: 136, E(): 0.33, (25.15% identity in 195 aa overlap). Has some hydrophobic stretches and contains bacterial regulatory proteins, araC family signature (PS00041). P9WJB1 No cell wall and cell processes P9WJB1 GO:0005886,GO:0046677,GO:0006810,GO:0016021 P9WJB1 NC_000962.3 Mycobrowser_v5 CDS 601857 602786 . + 0 Rv0510 hemC Involved in porphyrin biosynthesis by the C5 pathway (at the fourth step). Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane preuroporphyrinogen in several discrete steps [catalytic activity: 4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH3]. Probable porphobilinogen deaminase HemC (PBG) (hydroxymethylbilane synthase) (HMBS) (pre-uroporphyrinogen synthase) Rv0510, (MTCY21C8.01-MTCY20G9.37), len: 309 aa. Probable hemC, hydroxymethylbilane synthase (porphobilinogen deaminase), equivalent to HEM3B|Q49808|HEM3_MYCLE porphobilinogen deaminase from Mycobacterium leprae (315 aa), FASTA scores: opt: 889, E(): 0, (88.1% identity in 159 aa overlap). Also highly similar to others e.g. Q9WX16|HE31_STRCO probable porphobilinogen deaminase from Streptomyces coelicolor (319 aa); Q9L6Q2|HEM3_SALTY porphobilinogen deaminase from Salmonella typhimurium (313 aa); etc. Belongs to the HMBS family. Cofactor: covalently binds a dipyrromethane cofactor to which the porphobilinogen subunits are ADDED. P9WMP3 No intermediary metabolism and respiration P64336 P9WMP3 GO:0018160,GO:0006779,GO:0004418 2.5.1.61 P9WMP3 NC_000962.3 Mycobrowser_v5 CDS 602819 604516 . + 0 Rv0511 hemD Possibly involved in the biosynthesis of siroheme and cobalamin [catalytic activity: 2 S-adenosyl-L-methionine + uroporphyrin III = 2 S-adenosyl-L-homocysteine + sirohydrochlorin]. Probable uroporphyrin-III C-methyltransferase HemD (uroporphyrinogen III methylase) (urogen III methylase) (SUMT) (urogen III methylase) (UROM) Rv0511, (MTCY21C8.02), len: 565 aa. Probable hemD (alternate gene name: cysG), uroporphyrin-III C-methyltransferase, highly similar to others e.g. CAC31936.1|AL583925 possible uroporphyrin-III C-methyltransferase from Mycobacterium leprae (563 aa); and S72909|CYSG from Mycobacterium leprae (472 aa), FASTA scores: opt: 1946, E(): 0, (83.3% identity in 472 aa overlap); T36265|5123662|CAB45351.1|AL079345 probable uroporphyrin-III C-methyltransferase from Streptomyces coelicolor (565 aa); and similar to others e.g. AAK00606.1|AF221100_3|AF221100 from Selenomonas ruminantium subsp. ruminantium (505 aa); etc. Also similar to Rv2071c and Rv2847c from Mycobacterium tuberculosis. Note that previously known as cysG. Q6MX34 No intermediary metabolism and respiration Q6MX34 Q6MX34 GO:0004851,GO:0004852,GO:0033014 2.1.1.107 Q6MX34 NC_000962.3 Mycobrowser_v5 CDS 614835 616223 . + 0 Rv0524 hemL Involved in porphyrin biosynthesis by the C5 pathway (at the second step) [catalytic activity: (S)-4-amino-5-oxopentanoate = 5-aminolevulinate]. Probable glutamate-1-semialdehyde 2,1-aminomutase HemL (GSA) (glutamate-1-semialdehyde aminotransferase) (GSA-at) Rv0524, (MTCY25D10.03), len: 462 aa. Probable hemL, glutamate-1-semialdehyde 2,1-aminomutase, equivalent to P46716|GSA_MYCLE glutamate-1-semialdehyde 2,1-aminomutase from Mycobacterium leprae (446 aa), FASTA scores: opt: 1532, E(): 0, (82.6% identity in 460 aa overlap). Also highly similar to others e.g. Q9F2S0|GSA_STRCO from Streptomyces coelicolor (438 aa); Q06774|GSA_PROFR from Propionibacterium freudenreichii (441 aa); etc. Contains PS00600 Aminotransferases class-III pyridoxal-phosphate attachment site. Belongs to class-III of pyridoxal-phosphate-dependent aminotransferases. Cofactor: pyridoxal phosphate. P9WMN9 No intermediary metabolism and respiration P63506 P9WMN9 GO:0042286,GO:0006779,GO:0030170,GO:0008483,GO:0005737 5.4.3.8 P9WMN9 NC_000962.3 Mycobrowser_v5 CDS 617493 618272 . + 0 Rv0527 ccdA Possibly involved in cytochrome C synthesis. Might transfer reducing equivalents across the cytoplasmic membrane, promoting efficient disulfide bond isomerization of proteins localized on the outer surface of the membrane. Possible cytochrome C-type biogenesis protein CcdA Rv0527, (MTCY25D10.06), len: 259 aa. Possible ccdA, cytochrome C-type biogenesis protein, integral membrane protein, equivalent to Q49810|B2168_C1_192|S72890 hypothetical protein from Mycobacterium leprae (262 aa), FASTA scores: opt: 1341, E(): 0, (79.0% identity in 262 aa overlap). Also highly similar to others e.g. CAC08380.1 (253 aa); CCDA_BACSU|P45706 cytochrome C-type biogenesis protein from Bacillus subtilis (235 aa), FASTA scores: opt: 307, E(): 7.4e-13, (30.4% identity in 237 aa overlap); etc. Seems to belong to the DSBD subfamily. Note that previously known as ccsA. L7N671 No intermediary metabolism and respiration Q7D9P9 L7N671 GO:0016020,GO:0055114,GO:0017004 NC_000962.3 Mycobrowser_v5 CDS 622329 622646 . + 0 Rv0531 Rv0531 Unknown Possible conserved membrane protein Rv0531, (MTCY25D10.10), len: 105 aa. Possible conserved membrane protein, highly similar to Y13803|MLB1306_1|CAA74131.1 hypothetical protein from Mycobacterium leprae (86 aa), FASTA scores: E(): 2.1e-24, (74.4% identity in 86 aa overlap); and NP_302557.1|NC_002677 putative membrane protein from Mycobacterium leprae (111 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06397 No cell wall and cell processes O06397 NC_000962.3 Mycobrowser_v5 CDS 624473 625480 . - 0 Rv0533c fabH Involved in fatty acid biosynthesis. Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. KAS III catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities [catalytic activity: acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]]. 3-oxoacyl-[acyl-carrier-protein] synthase III FabH (beta-ketoacyl-ACP synthase III) (KAS III) Rv0533c, (MTCY25D10.12c), len: 335 aa. FabH (alternate gene name: mtFabH), 3-oxoacyl-[acyl-carrier protein] synthase III (see citations below), highly similar to others e.g. Q54206|FABH from streptomyces glaucescens (333 aa), FASTA scores: opt: 1109, E(): 0, (51.4% identity in 333 aa overlap); FABH_ECOLI|P24249 3-oxoacyl-[acyl-carrier-protein] synthase III (317 aa), FASTA scores: opt: 666, E(): 0, (37.1% identity in 318 aa overlap); etc. Belongs to the FabH family. P9WNG3 No lipid metabolism 1HZP,1M1M,1U6E,1U6S,2AHB,2AJ9,2QNX,2QNY,2QNZ,2QO0,2QO1,2QX1 P0A574 P9WNG3 GO:0033818,GO:0005737,GO:0006633,GO:0004315 2.3.1.180 P9WNG3 NC_000962.3 Mycobrowser_v5 CDS 626457 627251 . + 0 Rv0535 pnp Phosphorylates 5'-methylthioadenosin [catalytic activity: 5'-methylthioadenosine + phosphate = adenine + 5-methylthio-D-ribose 1-phosphate]. Probable 5'-methylthioadenosine phosphorylase Pnp (MTA phosphorylase) Rv0535, (MTCY25D10.14c), len: 264 aa. Probable pnp, 5'-methylthioadenosine phosphorylase, highly similar to others e.g. CAB90972.1|AL355832 putative methylthioadenosine phosphorylase from Streptomyces coelicolor (280 aa); etc. Also similar to Rv3307|deoD probable purine nucleoside phosphorylase from Mycobacterium tuberculosis (268 aa). Belongs to the PNP/MTAP family 2 of phosphorylases. Gene name could be inappropriate. O06401 No intermediary metabolism and respiration O06401 O06401 GO:0009116,GO:0017061 2.4.2.28 O06401 NC_000962.3 Mycobrowser_v5 CDS 627248 628288 . + 0 Rv0536 galE3 Possibly involved in galactose metabolism [catalytic activity: UDP-glucose = UDP-galactose]. Probable UDP-glucose 4-epimerase GalE3 (galactowaldenase) (UDP-galactose 4-epimerase) (uridine diphosphate galactose 4-epimerase) (uridine diphospho-galactose 4-epimerase) Rv0536, (MTCY25D10.15), len: 346 aa. Possible galE3, UDP-glucose 4-epimerase, highly similar to CAB76986.1|AL159178 putative epimerase from Streptomyces coelicolor (334 aa); and similar to other epimerases e.g. NP_436775.1|NC_003078 putative NDP-glucose dehydrataseepimerase protein from Sinorhizobium meliloti (368 aa); AF143772|AF143772_7 GepiA from Mycobacterium avium strain 2151 (353 aa), FASTA scores: opt: 577, E(): 3.9e-29, (36.6% identity in 352 aa overlap); GALE_METJA|Q57664 putative UDP-glucose 4-epimerase (305 aa), FASTA scores: opt: 300, E(): 1.6e-12, (30.9% identity in 343 aa overlap); etc. Also similar to Mycobacterium tuberculosis proteins e.g. Rv3634c, Rv3784, etc. Seems to belong to the sugar epimerase family. Note that previously known as galE2. L7N670 No intermediary metabolism and respiration Q7D9P4 L7N670 GO:0005975,GO:0044237,GO:0050662,GO:0003978 5.1.3.2 L7N670 NC_000962.3 Mycobrowser_v5 CDS 634416 635504 . - 0 Rv0542c menE Involved in menaquinone biosynthesis. O-succinylbenzoic acid (OSB) to O-succinylbenzoyl-CoA (OSB-CoA) [catalytic activity: ATP + O-succinylbenzoate + CoA = AMP + diphosphate + O-succinylbenzoyl-CoA]. Possible O-succinylbenzoic acid--CoA ligase MenE (OSB-CoA synthetase) (O-succinylbenzoate-CoA synthase) Rv0542c, (MTCY25D10.21c), len: 362 aa. Possible menE, O-succinylbenzoic acid-CoA ligase, highly similar to Q50170|AAA63145.1|U15187|XCLB 4-Coumarate--CoA ligase from Mycobacterium leprae (352 aa), FASTA scores: opt: 1815, E(): 0, (78.9% identity in 351 aa overlap). Also similar to N-terminus of acid-CoA ligases e.g. NP_471116.1|NC_003212 O-succinylbenzoic acid-CoA ligase from Listeria innocua (469 aa); NP_390957.1|NC_000964 O-succinylbenzoic acid-CoA ligase from Bacillus subtilis (486 aa); MENE_HAEIN|P44565 O-succinylbenzoic acid-CoA ligase from Haemophilus influenzae (452 aa), FASTA scores: opt: 307, E(): 4.6e-12, (25.4% identity in 339 aa overlap); etc. Also some similarity with fadD proteins from Mycobacterium tuberculosis. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family. P9WQ39 No intermediary metabolism and respiration O06408 P9WQ39 GO:0008756,GO:0008152 6.2.1.26 P9WQ39 NC_000962.3 Mycobrowser_v5 CDS 635935 636213 . - 0 Rv0544c Rv0544c Unknown Possible conserved transmembrane protein Rv0544c, (MTCY25D10.23c), len: 92 aa. Possible conserved transmembrane protein, equivalent to NP_302470.1|NC_002677 possible membrane protein from Mycobacterium leprae (96 aa); and shows some similarity to MLU15187_33|Q50172|U296V from Mycobacterium leprae (36 aa), FASTA scores: opt: 151, E(): 2.1e-05, (71.4% identity in 35 aa overlap). Also some similarity with VATL_NEPNO|Q26250 vacuolar ATP synthase 16 kDa proteolipid from Nephrops norvegicus (159 aa), FASTA scores: opt: 80, E(): 11, (26.1% identity in 88 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06410 No cell wall and cell processes O06410 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 636210 637463 . - 0 Rv0545c pitA Involved in low-affinity inorganic phosphate transport across the membrane. Responsible for the translocation of the substrate across the membrane. Probable low-affinity inorganic phosphate transporter integral membrane protein PitA Rv0545c, (MTCY25D10.24c), len: 417 aa. Probable pitA, low-affinity inorganic phosphate transporter, integral membrane protein, equivalent to Q50173|NP_302471.1 pitA from Mycobacterium leprae (414 aa), FASTA scores: opt: 2035, E(): 0, (76.3% identity in 418 aa overlap). Also highly similar to others e.g. CAB59461.1|AL132644 putative low-affinity phosphate transport protein from Streptomyces coelicolor (423 aa); PITA_ECOLI|P37308 low-affinity inorganic phosphate transporter from Escherichia coli (499 aa), FASTA scores: opt: 304, E(): 6.9e-10, (32.5 % identity in 234 aa overlap); etc. Belongs to the PHO-4 family of transporters, pit subfamily. P9WIA7 No cell wall and cell processes O06411 P9WIA7 GO:0016021,GO:0006817,GO:0005886,GO:0005315 P9WIA7 NC_000962.3 Mycobrowser_v5 CDS 641096 642811 . - 0 Rv0551c fadD8 Function unknown, but involved in lipid degradation. Probable fatty-acid-CoA ligase FadD8 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv0551c, (MTCY25D10.30c), len: 571 aa. Probable fadD8, fatty-acid-CoA synthetase, similar to many e.g. LCFA_ECOLI|P29212 long-chain-fatty-acid--CoA ligase (561 aa), FASTA scores: opt: 585, E(): 9.5e-30, (28.7% identity in 536 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Note other possible start sites exist downstream of this start. O06417 No lipid metabolism O06417 O06417 GO:0008152,GO:0016874 6.2.1.- O06417 NC_000962.3 Mycobrowser_v5 CDS 688032 688859 . + 0 Rv0590 mce2B Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce2B Rv0590, (MTCY19H5.32c), len: 275 aa. Mce2B; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07414|Rv0170|MTCI28.10|mce1B (346 aa); O53968|Rv1967|MTV051.05|mce3B (342 aa); etc. Also highly similar to others e.g. NP_302657.1|NC_002677 putative secreted protein from Mycobacterium leprae (346 aa); P45391|YRBD_ECOLI hypothetical 19.6 kDa protein from Escherichia coli (183 aa), FASTA scores: opt: 160, E(): 0.00099, (28.3% identity in 166 aa overlap); P45029|YRBD_HAEIN|HI1085 hypothetical protein from Haemophilus influenzae (167 aa), FASTA scores: opt: 135, E():0.035, (25.9% identity in 143 aa overlap); etc. Contains possible N-terminal signal or anchor sequence. Predicted to be an outer membrane protein (See Song et al., 2008). O07788 No virulence, detoxification, adaptation O07788 O07788 O07788 NC_000962.3 Mycobrowser_v5 CDS 646298 647962 . + 0 Rv0555 menD Involved in menaquinone biosynthesis (at the first step) [catalytic activity 1: isochorismate + 2-ketoglutarate = 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate + pyruvate + CO(2)] [catalytic activity 2: 2-oxoglutarate = succinate semialdehyde + CO(2)]. Probable bifunctional menaquinone biosynthesis protein MenD : 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (SHCHC synthase) + 2-oxoglutarate decarboxylase (alpha- ketoglutarate decarboxylase) (KDC) Rv0555, (MTCY25D10.34), len: 554 aa. Probable menD, menaquinone biosynthesis protein, including 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase and 2-oxoglutarate decarboxylase activities. Equivalent to NP_302478.1|NC_002677 putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase / 2-oxoglutarate decarboxylase from Mycobacterium leprae (556 aa). Also similar to others e.g. MEND_BACSU|P23970 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase from Bacillus subtilis (548 aa), FASTA scores: opt: 488, E(): 2.3e-21, (34.3% identity in 545 aa overlap); etc. Cofactor: thiamine pyrophosphate. P9WK11 No intermediary metabolism and respiration O06421 P9WK11 GO:0000287,GO:0030145,GO:0009234,GO:0030976,GO:0070204 2.2.1.9 P9WK11 NC_000962.3 Mycobrowser_v5 CDS 649689 650393 . + 0 Rv0558 menH Involved in menaquinone biosynthesis (at the last step) converts DMKH2 into MKH2 [catalytic activity: S-adenosyl-L-methionine + demethylmenaquinol = S-adenosyl-L-homocysteine + menaquinol]. Probable ubiquinone/menaquinone biosynthesis methyltransferase MenH (2-heptaprenyl-1,4-naphthoquinone methyltransferase) Rv0558, (MTCY25D10.37), len: 234 aa. Probable menH (alternate gene name: menG), ubiquinone/menaquinone biosynthesis methlytransferase (2-heptaprenyl-1,4-naphthoquinone methyltransferase), equivalent to NP_302480.1|NC_002677 putative ubiquinone/menaquinone biosynthesis methyltransferase from Mycobacterium leprae (238 aa). Also highly similar to others e.g. CAB44537.1|AL078618|T34630 from Streptomyces coelicolor (231 aa); UBIE_ECOLI|P27851 from Escherichia coli strain K12 (251 aa), FASTA scores: opt: 421, E(): 1.2e-21, (43.2% identity in 227 aa overlap); GRC2_BACSU|P31113 from Bacillus subtilis (233 aa), FASTA scores: opt: 345, E(): 1.4e-16, (34.6% identity in 231 aa overlap); etc. Belongs to the UbiE family. Note that previously known as ubiE. P9WFR3 No intermediary metabolism and respiration P0A638 P9WFR3 GO:0008168,GO:0009234 2.1.1.- P9WFR3 NC_000962.3 Mycobrowser_v5 CDS 650407 650745 . - 0 Rv0559c Rv0559c Unknown Possible conserved secreted protein Rv0559c, (MTCY25D10.38c), len: 112 aa. Possible conserved secreted protein, similar to NP_302481.1|NC_002677 putative secreted protein from Mycobacterium leprae (112 aa). Also similar to Y08B_MYCTU|Q11048 hypothetical 11.6 kDa protein FASTA scores: opt: 111, E(): 011, (25.4% identity in 114 aa overlap). Contains possible N-terminal signal sequence. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WKL3 No cell wall and cell processes O06425 P9WKL3 P9WKL3 NC_000962.3 Mycobrowser_v5 CDS 653879 654739 . + 0 Rv0563 htpX Possibly involved in adaptation. Hydrolizes specific peptides and/or proteins. Probable protease transmembrane protein heat shock protein HtpX Rv0563, (MTV039.01, MTCY25D10.42), len: 286 aa. (alternative start at position 654006). Probable htpX, protease heat shock protein X (transmembrane protein), equivalent to NP_302484.1|NC_002677 putative peptidase from Mycobacterium leprae (287 aa). Also highly similar to others e.g. CAC08262.1|AL392146 putative peptidase from Streptomyces coelicolor (287 aa); NP_387431.1|NC_003047 putative protease transmembrane protein from Sinorhizobium meliloti (319 aa); NP_105051.1|NC_002678 heat shock protein (htpX) from Mesorhizobium loti (336 aa); NP_248692.1|NC_000909|U67608|MJU67608_8 heat shock protein HtpX, possibly protease (htpX) from Methanococcus jannaschii (284 aa), FASTA scores: opt: 660, E(): 0, (46.5 identity in 245 aa overlap). Continuation of MTCY25D10.42. Belongs to peptidase family M48 (zinc metalloprotease). Cofactor: Zinc. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P9WHS5 No virulence, detoxification, adaptation P65815 P9WHS5 GO:0004222,GO:0005886,GO:0006508,GO:0008270,GO:0016021 3.4.24.- NC_000962.3 Mycobrowser_v5 CDS 1265472 1266488 . - 0 Rv1138c Rv1138c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv1138c, (MTCI65.05c), len: 338 aa. Possible oxidoreductase, similar to Q9EWQ8 putative oxidoreductase from Streptomyces coelicolor (343 aa). Also similar to many Mycobacterium tuberculosis hypothetical proteins e.g. Rv1751|P72008|MTCY04C12.35 (412 aa), fasta scores: opt: 89, E(): 4.5e-09, (24.6% identity in 358 aa overlap). O06538 No intermediary metabolism and respiration O06538 O06538 GO:0004497,GO:0055114,GO:0009055 1.-.-.- O06538 NC_000962.3 Mycobrowser_v5 CDS 659450 660868 . + 0 Rv0568 cyp135B1 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Possible cytochrome P450 135B1 Cyp135B1 Rv0568, (MT0594, MTV039.06), len: 472 aa. Possible cyp135B1, cytochrome P450, similar to putative cytochrome P-450 monoxygenases and other cytochrome P-450 related enzymes e.g. P29980|CPXN_ANASP probable cytochrome P450 from Anabaena sp. strain PCC 7120 (459 aa), FASTA scores: opt: 525, E(): 7.2e-27, (31.9% identity in 417 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. Rv0327c|NP_214841.1|NC_000962|CYP135A1|MT0342|MTCY63.32c putative cytochrome P450 (449 aa), FASTA scores: opt: 1080, E(): 0, (40.5% identity in 444 aa overlap); Rv3685c|NP_218202.1|NC_000962 putative cytochrome P450 (476 aa); Rv0136|NP_214650.1|NC_000962 putative cytochrome P450 (441 aa); etc. Contains cytochrome P450 cysteine heme-iron ligand signature (PS00086). P9WPM9 No intermediary metabolism and respiration P63715 P9WPM9 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPM9 NC_000962.3 Mycobrowser_v5 CDS 661295 663373 . + 0 Rv0570 nrdZ Involved in the DNA replication pathway (at the first reaction). Provides the precursors necessary for DNA synthesis [catalytic activity: 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O = ribonucleoside diphosphate + reduced thioredoxin]. Probable ribonucleoside-diphosphate reductase (large subunit) NrdZ (ribonucleotide reductase) Rv0570, (MTV039.08), len: 692 aa. Probable nrdZ, ribonucleoside-diphosphate reductase, large subunit, highly similar to others e.g. NP_070492.1|NC_000917|NRD|AE000988_11 ribonucleotide reductase from Archaeoglobus fulgidus (752 aa), FASTA scores: opt: 2001, E(): 0, (52.5% identity in 562 aa overlap) (N-terminus shorter); U73619|TAU73619_1|T37459 ribonucleotide reductase from Thermoplasma acidophilum (857 aa), FASTA scores: opt: 1678, E(): 0, (43.7% identity in 723 aa overlap); etc. Belongs to the ribonucleoside diphosphate reductase large chain family. P9WH77 No information pathways O53767 P9WH77 GO:0006260,GO:0016960,GO:0031419,GO:0055114,GO:0005971,GO:0005524 1.17.4.1 P9WH77 NC_000962.3 Mycobrowser_v5 CDS 665851 667242 . - 0 Rv0573c pncB2 Involved in NAD salvage. Phosphoribosylation of nicotinic acid. [catalytic activity: nicotinate + 5-phosphoribosyl 1-pyrophosphate = nicotinate mononucleotide + diphosphate] Nicotinic acid phosphoribosyltransferase PncB2 Rv0573c, (MTV039.11c), len: 463 aa. PncB2, nicotinic acid phosphoribosyltransferase (See Boshoff et al., 2008). Similar to e.g. NP_213718.1|NC_000918 hypothetical protein from Aquifex aeolicus (426 aa); AL109962|T36953|SCJ1.20 conserved hypothetical protein from Streptomyces coelicolor (438 aa), FASTA scores: opt: 1089, E(): 0, (49.4% identity in 385 aa overlap); P_391053.1|Z99120|BSUB0017_57|NC_000964 protein similar to nicotinate phosphoribosyltransferase from Bacillus subtilis (490 aa), FASTA scores: opt: 955, E():0, (43.5% identity in 356 aa overlap); etc. Also similar to Q10641|Y03F_MYCTU|MTCY130.15c|Rv1330c conserved hypothetical protein from Mycobacterium tuberculosis (509 aa), FASTA scores: opt: 761, E(): 0, (38.4% identity in 437 aa overlap). P9WJI7 No intermediary metabolism and respiration O53770 P9WJI7 GO:0019363,GO:0004516 2.4.2.11 P9WJI7 NC_000962.3 Mycobrowser_v5 CDS 838451 840856 . + 0 Rv0747 PE_PGRS10 Function unknown PE-PGRS family protein PE_PGRS10 Rv0747, (MTV041.21), len: 801 aa. PE_PGRS10, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to part of MTCY28.25c|Rv1759c|Z95890 antigen wag22 from M. tuberculosis (914 aa), FASTA scores: opt: 2772, E(): 0, (60.9% identity in 941 aa overlap). Also similar to other PE-PGRS family proteins e.g. Z95844|MTCY493_2 FASTA score: (50.2% identity in 815 aa overlap). Contains PS00012 Phosphopantetheine attachment site. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WIG1 No PE/PPE O53810 P9WIG1 P9WIG1 NC_000962.3 Mycobrowser_v5 CDS 671996 675916 . - 0 Rv0578c PE_PGRS7 Function unknown PE-PGRS family protein PE_PGRS7 Rv0578c, (MTV039.16c), len: 1306 aa. PE_PGRS7, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to many other PGRS proteins e.g. MTCY493.04|Z95844 from Mycobacterium tuberculosis (1329 aa), FASTA scores: opt: 3994, E(): 0, (54.6% identity in 1375 aa overlap). Contains two PS00583 pfkB family of carbohydrate kinases signatures possibly fortuitously. Q6MX28 No PE/PPE Q6MX28 Q6MX28 Q6MX28 NC_000962.3 Mycobrowser_v5 CDS 678389 679075 . - 0 Rv0583c lpqN Unknown Probable conserved lipoprotein LpqN Rv0583c, (MTV039.21c), len: 228 aa. Probable lpqN, conserved lipoprotein, equivalent to AAA90989.1|U20446|MK35|U20446|MKU20446_1 lipoprotein precursor from Mycobacterium kansasii (225 aa), FASTA scores: opt: 945, E(): 0, (62.7% identity in 228 aa overlap); and similar to others from Mycobacteria e.g. Rv0040c and Rv1016c from Mycobacterium tuberculosis. Contains N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O53780 No cell wall and cell processes O53780 O53780 O53780 NC_000962.3 Mycobrowser_v5 CDS 681885 684272 . - 0 Rv0585c Rv0585c Unknown Probable conserved integral membrane protein Rv0585c, (MTV039.23c, MTCY19H5.37), len: 795 aa. Probable conserved integral membrane protein. C-terminus similar to CAB88984.1|AL353864 putative integral membrane protein from Streptomyces coelicolor (299 aa); and C-terminal region of CAC01311.1|AL390968 putative integral membrane protein from Streptomyces coelicolor (925 aa). Also some similarity with Rv0204 from Mycobacterium tuberculosis. O53781 No cell wall and cell processes O53781 O53781 NC_000962.3 Mycobrowser_v5 CDS 685928 686815 . + 0 Rv0588 yrbE2B Unknown Conserved hypothetical integral membrane protein YrbE2B Rv0588, (MTCY19H5.34c), len: 295 aa. YrbE2B, hypothetical unknown integral membrane protein, part of mce2 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07413|Rv0168|MTCI28.08|yrbE1B (289 aa); O53966|Rv1965|MTV051.03|yrbE3B (271 aa); etc. Also highly similar to conserved hypothetical integral membrane proteins of the yrbEB type, e.g. P45392|YRBE_ECOLI hypothetical 27.9 kDa protein from Escherichia coli (260 aa), FASTA scores: opt: 232, E(): 8.4e-08, (22.1 % identity in 267 aa overlap); P45030|YRBE_HAEIN|HI1086 hypothetical protein from Haemophilus influenzae (261 aa), FASTA scores: opt: 234, E(): 6.3e-08, (24.2% identity in 215 aa overlap); NP_302655.1|NC_002677 conserved membrane protein from Mycobacterium leprae (289 aa); etc. O07790 No virulence, detoxification, adaptation O07790 O07790 NC_000962.3 Mycobrowser_v5 CDS 686821 688035 . + 0 Rv0589 mce2A Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce2A Rv0589, (MTCY19H5.33c), len: 404 aa. Mce2A; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins P72013|MCE1|Rv0169|MTCI28.09|mce1A (454 aa); O53967|MCE3|Rv1966|MTV051.04|mce3A (425 aa); etc. Also highly similar to others e.g. AAD52105.1|AF113402_1|AF113402 mycobacterial cell entry protein from Mycobacterium bovis BCG (454 aa); NP_302656.1|NC_002677 putative cell invasion protein from Mycobacterium leprae (441 aa); CAC12798.1|AL445327 putative secreted protein from Streptomyces coelicolor (418 aa); etc. Also highly similar, but longer 21 aa, to P72013|CAA50257.1|X70901|MTCI28.08 Mcep protein from Mycobacterium tuberculosis (432 aa), FASTA scores: opt: 1324, E(): 0, (62.6% identity in 436 aa overlap). Contains a possible N-terminal signal or anchor sequence. Predicted to be an outer membrane protein (See Song et al., 2008). Note that previously known as mce2. Q79FY7 No virulence, detoxification, adaptation Q79FY7 Q79FY7 Q79FY7 NC_000962.3 Mycobrowser_v5 CDS 688808 689062 . + 0 Rv0590A Rv0590A Unknown, but could be involved in host cell invasion. Mce-family related protein Rv0590A, len: 84 aa. Probable continuation of mce2B|Rv0590. Can find no frameshift to account for this. Possible nucleotide G missing at 688793 as there are 5 in Mycobacterium bovis but only 4 in CDC1551. Strong similarity to C-terminus of other Mce proteins e.g. AL583926|AL583926_38 from Mycobacterium leprae strain tn (346 aa), FASTA scores: E(): 1.2e-20, (67.85% identity in 84 aa overlap). I6X9D2 No virulence, detoxification, adaptation I6X9D2 NC_000962.3 Mycobrowser_v5 CDS 689059 690504 . + 0 Rv0591 mce2C Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce2C Rv0591, (MTCY19H5.31c), len: 481 aa. Mce2C; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07415|R0171|MTCI28.11|mce1C (515 aa); O53969|Rv1968|MTV051.06|mce3C (410 aa); etc. Also highly similar to others e.g. NP_302658.1|NC_002677 putative secreted protein from Mycobacterium leprae (519 aa); CAC12796.1|AL445327 putative secreted protein from Streptomyces coelicolor (351 aa); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and may contain N-terminal signal or anchor sequence. Has highly Pro-rich C-terminus. O07787 No virulence, detoxification, adaptation O07787 O07787 O07787 NC_000962.3 Mycobrowser_v5 CDS 690501 692027 . + 0 Rv0592 mce2D Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce2D Rv0592, (MTCY19H5.30c), len: 508 aa. Mce2D; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07416|Rv0172|MTCI28.12|mce1D (530 aa); O53970|Rv1969|MTV051.07|mce3D (423 aa); etc. Also highly similar to others e.g. NP_302659.1|NC_002677 putative secreted protein from Mycobacterium leprae (531 aa); CAC12795.1|AL445327 putative secreted protein from Streptomyces coelicolor (337 aa); etc. Has highly Pro-rich C-terminus and may contain N-terminal signal or anchor sequence. Predicted to be an outer membrane protein (See Song et al., 2008). I6WYT7 No virulence, detoxification, adaptation O07786 I6WYT7 I6WYT7 NC_000962.3 Mycobrowser_v5 CDS 692024 693232 . + 0 Rv0593 lprL Unknown, but thought to be involved in host cell invasion. Possible Mce-family lipoprotein LprL (Mce-family lipoprotein Mce2E) Rv0593, (MTCY19H5.29c), len: 402 aa. Possible lprL (alternate gene name: mce2E), lipoprotein which belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07417|LPRK|Rv0173|MTCI28.13|mce1E (390 aa); O53971|LPRM|Rv1970|MTV051.08|mce3E (377 aa); etc. Also highly similar to others e.g. NP_302660.1|NC_002677 putative lipoprotein from Mycobacterium leprae (392 aa); CAC12794.1|AL445327 putative secreted protein from Streptomyces coelicolor (413 aa); etc. Contains possible signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site. I6Y461 No cell wall and cell processes O07785 I6Y461 I6Y461 NC_000962.3 Mycobrowser_v5 CDS 693237 694787 . + 0 Rv0594 mce2F Unknown, but thought involved in host cell invasion. Mce-family protein Mce2F Rv0594, (MTCY19H5.28c), len: 516 aa. Mce2F; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), similar to Mycobacterium tuberculosis proteins O07418|Rv0174|MTCI28.14|mce1F (515 aa); O53972|Rv1971|MTV051.09|mce3F (437 aa); etc. Also highly similar to others e.g. NP_302661.1|NC_002677 putative secreted protein from Mycobacterium leprae (516 aa); AAF74993.1|AF143400_1|AF143400|996A027a protein from Mycobacterium avium (80 aa) (similarity on C-terminus); CAC12793.1|AL445327 putative secreted protein from Streptomyces coelicolor (433 aa); etc. Contains possible N-terminal signal or anchor sequence. Predicted to be an outer membrane protein (See Song et al., 2008). O07784 No virulence, detoxification, adaptation O07784 O07784 O07784 NC_000962.3 Mycobrowser_v5 CDS 694839 695231 . - 0 Rv0595c vapC4 Unknown Possible toxin VapC4 Rv0595c, (MTCY19H5.27), len: 130 aa. Possible vapC4, toxin, part of toxin-antitoxin (TA) operon with Rv0596c, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to other conserved hypothetical proteins e.g. Rv0627 (135 aa) and Rv0665 (112 aa) from Mycobacterium tuberculosis; and STBB_PSESM|Q52562 plasmid stability protein from Pseudomonas syringae (139 aa), FASTA scores: opt: 131, E(): 0.0035, (35.2% identity in 88 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07783 No virulence, detoxification, adaptation O07783 O07783 O07783 NC_000962.3 Mycobrowser_v5 CDS 695228 695485 . - 0 Rv0596c vapB4 Unknown Possible antitoxin VapB4 Rv0596c, (MTCY19H5.26), len: 85 aa. Possible vapB4, antitoxin, part of toxin-antitoxin (TA) operon with Rv0595c (See Arcus et al., 2005; Pandey and Gerdes, 2005). Highly similar in part to other M. tuberculosis hypothetical proteins e.g. Rv0626, Rv3181c, Rv3385c, Rv3407, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WF21 No virulence, detoxification, adaptation O07782 P9WF21 P9WF21 NC_000962.3 Mycobrowser_v5 CDS 695668 696903 . - 0 Rv0597c Rv0597c Function unknown Conserved hypothetical protein Rv0597c, (MTCY19H5.25), len: 411 aa. Conserved hypothetical protein, highly similar to Rv3179 conserved hypothetical protein from Mycobacterium tuberculosis (429 aa). Also similar to AAF76191.1|AF271296_1|AF271296 putative ATP/GTP binding protein from Mycobacterium smegmatis (428 aa); Rv2008c|YW09_MYCTU|Q10849 conserved hypothetical protein from Mycobacterium tuberculosis (441 aa), FASTA scores: opt: 270, E(): 3.6e-11, (30.5% identity in 416 aa overlap) (N-terminus longer). Also similar to other hypothetical proteins e.g. NP_085874.1|NC_002679 hypothetical protein from Mesorhizobium loti (435 aa) (N-terminus longer). Contains PS00017 ATP/GTP-binding site motif A (P-loop). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6WYU2 No conserved hypotheticals I6WYU2 I6WYU2 NC_000962.3 Mycobrowser_v5 CDS 697564 697800 . - 0 Rv0599c vapB27 Unknown Possible antitoxin VapB27 Rv0599c, (MTCY19H5.23), len: 78 aa. Possible vapB27, antitoxin, part of toxin-antitoxin (TA) operon with Rv0598c, see Arcus et al. 2005. Similar to others e.g. Rv2595|Y0B6_MYCTU|Q50626 conserved hypothetical protein from Mycobacterium tuberculosis (81 aa), FASTA scores: opt: 160, E(): 6.2e-07, (35.8% identity in 81 aa overlap). N-terminus shows stong similarity with N-terminus of NP_104908.1|NC_002678 hypothetical protein from Mesorhizobium loti (89 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07779 No virulence, detoxification, adaptation O07779 O07779 O07779 NC_000962.3 Mycobrowser_v5 CDS 697904 698410 . - 0 Rv0600c Rv0600c Sensor part of a two component regulatory system. Two component sensor kinase [second part] Rv0600c, (MTCY19H5.22), len: 168 aa (probable partial CDS). Two-component sensor kinase (second part), similar to part (C-termini) of many others e.g. Q04943|AFQ2_STRCO sensor protein afsq2 from Streptomyces coelicolor (535 aa), FASTA scores: opt: 347, E(): 1.9e-12, (33.0% identity in 206 aa overlap); etc. Note that sequence was checked and no errors were detected, which would allow this and the upstream ORF to be joined. Start changed since first submission (- 39 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07778 No regulatory proteins O07778 O07778 GO:0018106,GO:0007165,GO:0000155,GO:0000160,GO:0005524 2.7.13.3 O07778 NC_000962.3 Mycobrowser_v5 CDS 699856 700167 . + 0 Rv0603 Rv0603 Unknown Possible exported protein Rv0603, (MTCY19H5.19c), len: 103 aa. Possible exported protein with hydrophobic stretch at aa 7-29. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07775 No cell wall and cell processes 2KGY O07775 O07775 NC_000962.3 Mycobrowser_v5 CDS 700239 701189 . + 0 Rv0604 lpqO Unknown Probable conserved lipoprotein LpqO Rv0604, (MTCY19H5.18c), len: 316 aa. Probable lpqO, conserved lipoprotein, highly similar to Rv2999|lppY putative lipoprotein from Mycobacterium tuberculosis (321 aa), FASTA scores: opt: 1153, E(): 0, (53.2% identity in 312 aa overlap). Contains probable N-terminal signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6X9E2 No cell wall and cell processes O07774 I6X9E2 NC_000962.3 Mycobrowser_v5 CDS 701406 702014 . + 0 Rv0605 Rv0605 Prevents the cointegration of foreign DNA before integration into the chromosome. Possible resolvase Rv0605, (MTCY19H5.17c), len: 202 aa. Possible resolvase for IS_Y349 element, similar to several Mycobacterial hypothetical proteins and weakly similar to Q52563 resolvase from Pseudomonas syringae (210 aa), FASTA scores: opt: 99, E(): 3.1, (35.7% identity in 98 aa overlap). Contains PS00397 Site-specific recombinases active site and probable helix-turn helix motif from aa 9-30 (Score 1815, +5.37 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6Y890 No insertion seqs and phages O07773 I6Y890 GO:0006310,GO:0000150,GO:0003677 I6Y890 NC_000962.3 Mycobrowser_v5 CDS 702016 702759 . + 0 Rv0606 Rv0606 Thought to be required for the transposition of the insertion element IS_1536. Possible transposase (fragment) Rv0606, (MTCY19H5.16c), len: 247 aa. Possible truncated transposase for IS_1536 element, highly similar to N-terminus of other transposases from Mycobacterium tuberculosis e.g. YX16_MYCTU|Q10809|Rv2885c|MT2953|MTCY274.16c putative transposase from Mycobacterium tuberculosis (460 aa), FASTA scores: opt: 1368, E(): 0, (83.5% identity in 237 aa overlap); Rv2978c, Rv0922, Rv3827c, etc. Also similar to N-terminus of MTV002_57|Rv2792 resolvase from M. tuberculosis (193 aa), FASTA score: (87.4% identity in 238 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07772 No insertion seqs and phages O07772 O07772 NC_000962.3 Mycobrowser_v5 CDS 703486 703887 . + 0 Rv0609 vapC28 Unknown Possible toxin VapC28. Contains PIN domain. Rv0609, (MTCY19H5.13c), len: 133 aa. Possible vapC28, toxin, part of toxin-antitoxin (TA) operon with Rv0608, contains PIN domain, see Arcus et al. 2005. Similar to several Mycobacterium tuberculosis hypothetical proteins e.g. YW37_MYCTU|Q10874|Rv1982c|MT2034|MTCY39.37 conserved hypothetical protein (139 aa), FASTA scores: opt: 262, E(): 8.1e-12, (39.1% identity in 128 aa overlap); MTCY20H10.05|Rv0624|MT0652|MTCY20H10.05 conserved hypothetical protein (131 aa), FASTA score: (42.9% identity in 126 aa overlap), Rv0565c, Rv3854c, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WF81 No virulence, detoxification, adaptation P67238 P9WF81 P9WF81 NC_000962.3 Mycobrowser_v5 CDS 706324 706929 . + 0 Rv0612 Rv0612 Function unknown Conserved hypothetical protein Rv0612, (MTCY19H5.09c), len: 201 aa. Conserved hypothetical protein, highly similar, but in part, to downstream ORF Rv0609A conserved hypothetical protein from Mycobacterium tuberculosis (75 aa); and showing weak similarity with other hypothetical proteins from Mycobacterium tuberculosis. Note that first start has been taken although this overlaps slightly with the upstream ORF. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6X9E8 No conserved hypotheticals I6X9E8 NC_000962.3 Mycobrowser_v5 CDS 706948 709515 . - 0 Rv0613c Rv0613c Unknown Unknown protein Rv0613c, (MTCY19H5.08), len: 855 aa. Unknown protein. Contains a very short region with strong similarity to several preprotein translocases e.g. P47847|SECA_LISMO preprotein translocase seca subunit (836 aa), FASTA scores: opt: 138, E(): 0.18, (38.6% identity in 70 aa overlap, and 72.7% identity in 22 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6Y897 No conserved hypotheticals O07764 I6Y897 I6Y897 NC_000962.3 Mycobrowser_v5 CDS 709356 710348 . + 0 Rv0614 Rv0614 Function unknown Conserved hypothetical protein Rv0614, (MTCY19H5.07c), len: 330 aa. Conserved hypothetical protein, similar in part to Mycobacterium tuberculosis hypothetical proteins e.g. YY16_MYCTU|Q10685|Rv2077c|MT2137|MTCY49.16c conserved hypothetical protein (323 aa), FASTA scores: opt: 200, E(): 0.00016, (28.3% identity in 269 aa overlap); MTCY9F9_15 FASTA score: (40.3% identity in 144 aa overlap), Rv1949c, Rv2542, etc. Several start sites are possible; first start has been chosen. Note that this ORF overlaps with the upstream ORF. Predicted to be an outer membrane protein (See Song et al., 2008). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07763 No conserved hypotheticals O07763 NC_000962.3 Mycobrowser_v5 CDS 712716 713807 . + 0 Rv0620 galK Involved in galactose metabolism (leloir pathway) (at the first reaction) [catalytic activity: ATP + D-galactose = ADP + D-galactose 1-phosphate]. Probable galactokinase GalK (galactose kinase) Rv0620, (MTCY19H5.01c, MTCY20H10.01), len: 363 aa. Probable galK, galactokinase, similar to others e.g. P13227|GAL1_STRLI galactokinase from Streptomyces lividans (397 aa); P06976|GAL1_ECOLI galactokinase from Escherichia coli (381 aa), FASTA scores: opt: 669, E(): 0, (35.9% identity in 365 aa overlap); etc. Contains PS00106 Galactokinase signature and PS00560 Serine carboxypeptidases, histidine active site. Belongs to the GHMP kinase family. GALK subfamily. P9WN63 No intermediary metabolism and respiration P96910 P9WN63 GO:0046835,GO:0005737,GO:0004335,GO:0006012,GO:0005524 2.7.1.6 P9WN63 NC_000962.3 Mycobrowser_v5 CDS 791070 791666 . - 0 Rv0691c Rv0691c Involved in transcriptional mechanism Probable transcriptional regulatory protein Rv0691c, (MTCY210.08c), len: 198 aa. Probable transcriptional regulator, highly similar to AAC77476.1|U17129 unknown protein from Rhodococcus erythropolis (185 aa); and showing similarity with putative regulatory proteins eg STMTCREP_1|TCMR_STRGA|P39885 tetracenomycin c transcriptional repressor from Streptomyces glaucescens (226 aa), FASTA scores: opt: 178, E(): 8.5e-06, (27.9% identity in 201 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop) and probable helix-turn helix motifs from aa 34-55 (Score 1100, +2.93 SD) and 151-172 (Score 1124, +3.02 SD). P9WMB7 No regulatory proteins P95037 P9WMB7 GO:0006350,GO:0003700,GO:0006355 P9WMB7 NC_000962.3 Mycobrowser_v5 CDS 720005 721732 . - 0 Rv0629c recD Involved in homologous recombination. Probable exonuclease V (alpha chain) RecD (exodeoxyribonuclease V alpha chain) (exodeoxyribonuclease V polypeptide) Rv0629c, (MTCY20H10.10c), len: 575 aa. Probable recD, exonuclease V, alpha chain (exodeoxyribonuclease V, alpha chain) (see citation below), highly similar to other exonucleases e.g. AF157643_3|AAD46809.1|recD Escherichia coli RecD protein homolog from Mycobacterium smegmatis (554 aa); P04993|EX5A_ECOLI|B2819 exodeoxyribonuclease V 67kd polypeptide (exonuclease V alpha chain) from Escherichia coli strain K12 (608 aa), FASTA scores: opt: 512, E(): 1.9e-24, (36.9% identity in 582 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Consists of three subunits; RECB|Rv0630c, RECC|Rv0631c and RECD. P9WHJ1 No information pathways P96919 P9WHJ1 GO:0006281,GO:0004519,GO:0008854,GO:0009338,GO:0004386,GO:0005524 3.1.11.5 P9WHJ1 NC_000962.3 Mycobrowser_v5 CDS 725013 728306 . - 0 Rv0631c recC Involved in homologous recombination. Probable exonuclease V (gamma chain) RecC (exodeoxyribonuclease V gamma chain)(exodeoxyribonuclease V polypeptide) Rv0631c, (MTCY20H10.12c), len: 1097 aa. Probable recC, exonuclease V, gamma chain (exodeoxyribonuclease V, gamma chain) (see Mizrahi & Andersen 1998), highly similar to other exonucleases e.g. AF157643_1|RecC|AAD46807.1 Escherichia coli RecC protein homolog from Mycobacterium smegmatis (1085 aa); P07648|EX5C_ECOLI|B2822 exodeoxyribonuclease V 125 kDa polypeptide (exonuclease V gamma chain) from Escherichia coli strain K12 (1122 aa), FASTA scores: opt: 954, E(): 0, (29.2% identity in 1109 aa overlap); etc. Consists of three subunits; RECB|Rv0630c, RECC and recd|Rv0629c. The transcription of this CDS seems to be activated specifically in host granulomas (see Ramakrishnan et al., 2000). P9WIQ5 No information pathways P9WIQ5 GO:0006281,GO:0004519,GO:0008854,GO:0009338,GO:0004386,GO:0005524 3.1.11.5 P9WIQ5 NC_000962.3 Mycobrowser_v5 CDS 728583 729278 . - 0 Rv0632c echA3 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Probable enoyl-CoA hydratase EchA3 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv0632c, (MTCY20H10.13c), len: 231 aa. Probable echA3, enoyl-CoA hydratase, almost identical to the MTU88877_1 enoyl-CoA hydratase of Mycobacterium tuberculosis field isolate NTI64719, FASTA score: (92.4% identity in 184 aa overlap). Also similar to others e.g. P24162|ECHH_RHOCA enoyl-CoA hydratase from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (257 aa), FASTA scores: opt: 206, E(): 6.3e-07, (31.5% identity in 232 aa overlap); etc. I6Y8B5 No lipid metabolism P96907 I6Y8B5 GO:0016853,GO:0008152,GO:0004300 4.2.1.17 I6Y8B5 NC_000962.3 Mycobrowser_v5 CDS 730320 731033 . - 0 Rv0634c Rv0634c Thought to be involved in glyoxal pathway. Thiolesterase that catalyses the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid [catalytic activity: (S)-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy acid anion]. Possible glyoxalase II (hydroxyacylglutathione hydrolase) (GLX II) Rv0634c, (MTCY20H10.15c), len: 237 aa. Possible glyoxalase II, equivalent to NP_302290.1|NC_002677 putative glyoxylase II from Mycobacterium leprae (238 aa); and similar to U00011_3|Y0BK_MYCLE|Q49649 hypothetical 23.9 kDa protein from Mycobacterium leprae (218 aa), FASTA scores: opt: 281, E(): 3.9e-12, (31.8% identity in 201 aa overlap). Also similar to other glyoxalases and metallo-beta-lactamase family proteins e.g. NP_386770.1|NC_003047 putative hydroxyacylglutathione hydrolase from Sinorhizobium meliloti (256 aa); etc. Also similar to other putative glyoxylases from Mycobacterium tuberculosis e.g. Rv1637c. Belongs to the glyoxalase II family. Cofactor: binds two zinc ions. I6Y4A5 No virulence, detoxification, adaptation I6Y4A5 GO:0004416 3.1.2.6 I6Y4A5 NC_000962.3 Mycobrowser_v5 CDS 731113 731364 . + 0 Rv0634A Rv0634A Unknown Unknown protein Rv0634A, len: 83 aa. Unknown protein. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WKS5 No conserved hypotheticals Q8VKH3 P9WKS5 P9WKS5 NC_000962.3 Mycobrowser_v5 CDS 732825 733325 . + 0 Rv0637 hadC Involved in fatty acid synthesis type II (fas-II) (3R)-hydroxyacyl-ACP dehydratase subunit HadC Rv0637, (MTCY20H10.18), len: 166 aa. HadC, (3R)-hydroxyacyl-ACP dehydratase subunit, equivalent to NP_302285.1|NC_002677|YV31_MYCLE|P54879 conserved hypothetical protein from Mycobacterium leprae (166 aa), FASTA scores: opt: 352, E(): 4e-19, (39.2% identity in 148 aa overlap); and highly similar to others from Mycobacterium leprae e.g. NP_302287.1|NC_002677 conserved hypothetical protein (159 aa). Also highly similar to CAB77410.1|AL160431|SCD82.07 hypothetical protein from Streptomyces coelicolor (150 aa); Rv0635|NP_215149.1|NC_000962|MTY20H10_17 conserved hypothetical protein (two ORFs upstream) from Mycobacterium tuberculosis (158 aa), FASTA score: (49.3% identity in 150 aa overlap); and Rv0504c|NP_215018.1|NC_000962|YV31_MYCTU|Q11168 hypothetical protein from Mycobacterium tuberculosis (166 aa), FASTA scores: opt: 380, E(): 3.8e-21, (43.1% identity in 137 aa overlap). P9WFJ9 No intermediary metabolism and respiration P9WFJ9 P9WFJ9 NC_000962.3 Mycobrowser_v5 CDS 735517 736224 . + 0 Rv0641 rplA This protein binds directly to 23S ribosomal RNA and is located in the neighborhood of the site where elongation factor TU is bound to the ribosome. 50S ribosomal protein L1 RplA Rv0641, (MTCY20H10.22), len: 235 aa. rplA, 50S ribosomal protein L1, equivalent to NP_302281.1|NC_002677 50S ribosomal protein L1 from Mycobacterium leprae (235 aa). Also highly similar to others e.g. P3625|RL1_STRGR 50s ribosomal protein L1 from Streptomyces griseus (240 aa), FASTA scores: opt: 1081, E(): 0, (72.2% identity in 230 aa overlap); etc. Belongs to the L1P family of ribosomal proteins. P9WHC7 No information pathways P96932 P9WHC7 GO:0015934,GO:0019843,GO:0006417,GO:0003735,GO:0000049,GO:0006412,GO:0006396 P9WHC7 NC_000962.3 Mycobrowser_v5 CDS 1894224 1895342 . - 0 Rv1668c Rv1668c Thought to be involved in active transport of macrolide across the membrane (export). Macrolide antibiotics resistance by an export mechanism. Responsible for energy coupling to the transport system. Probable first part of macrolide-transport ATP-binding protein ABC transporter Rv1668c, (MTV047.04c), len: 372 aa. Probable first part of macrolide-transport ATP-binding protein ABC transporter (see citation below), similar to many ATP-binding proteins ABC transporter e.g. X80735|SEABCT_1|Q54072 Saccharopolyspora erythraea ertX gene (481 aa), FASTA scores: opt: 938, E(): 0, (45.6% identity in 353 aa overlap); etc. Similarity to other NBD components of ABC transporters suggests that Rv1667c and Rv1668c should be contiguous. However, sequence has been checked and no error found, also same sequence in Mycobacterium tuberculosis CSU93 and Mycobacterium bovis. Contains PS00211 ABC transporters family signature and two times PS00017 ATP/GTP-binding site motif A. Belongs to the ATP-binding transport protein family (ABC transporters). O53916 No cell wall and cell processes O53916 O53916 GO:0016887,GO:0005524 O53916 NC_000962.3 Mycobrowser_v5 CDS 736298 737203 . - 0 Rv0642c mmaA4 Involved in mycolic acids modification. Catalyzes unusual S-adenosyl-methionine-dependent transformation of a cis-olefin mycolic acid into a secondary alcohol. Catalyzes introduction of a hydroxyl group at the distal position on mycolic acid chains to produce the hydroxyl mycolate. Mycolic acids represent a major constituent of the mycobacterial cell wall complex. Methyl transfer results in formation of a secondary hydroxy group with an adjacent methyl branch; olefinic mycolic acid methyl transferase. Methoxy mycolic acid synthase 4 MmaA4 (methyl mycolic acid synthase 4) (MMA4) (hydroxy mycolic acid synthase) Rv0642c, (MTCY20H10.23c), len: 301 aa. MmaA4, methoxy mycolic acid synthase 4 (methyltransferase) (see citations below). Equivalent to AAC44876|AAC44876.1|cmaA methyl transferase (mycolic acid modification protein) from Mycobacterium bovis BCG strain Pasteur (298 aa); NP_302280.1|NC_002677 methyl mycolic acid synthase 4 from Mycobacterium leprae (298 aa); and highly similar to others from Mycobacteria e.g. downstream ORF P72027|mmaA3|Rv0643c|MTCY20H10.24c putative methoxy mycolic acid synthase 3 from Mycobacterium tuberculosis (293 aa). Phosphorylated in vitro by PknJ|Rv2088 (See Jang et al., 2010). Q79FX8 No lipid metabolism 2FK7,2FK8,3HA3,3HA5,3HA7 Q79FX8 Q79FX8 GO:0008610,GO:0008825 2.1.1.- Q79FX8 NC_000962.3 Mycobrowser_v5 CDS 739327 740187 . - 0 Rv0645c mmaA1 Involved in mycolic acids modification. Catalyzes unusual S-adenosyl-methionine-dependent transformation of a cis-olefin mycolic acid into a secondary alcohol. Catalyzes introduction of a hydroxyl group at the distal position on mycolic acid chains to produce the hydroxyl mycolate. Mycolic acids represent a major constituent of the mycobacterial cell wall complex. Methyl transfer results in formation of a secondary hydroxy group with an adjacent methyl branch; olefinic mycolic acid methyl transferase. Methoxy mycolic acid synthase 1 MmaA1 (methyl mycolic acid synthase 1) (MMA1) (hydroxy mycolic acid synthase) Rv0645c, (MTCY20H10.26c), len: 286 aa. MmaA1, methoxy mycolic acid synthase 1 (methyltransferase) (see citations below). Equivalent to NP_302279.1|NC_002677 methyl mycolic acid synthase 1 from Mycobacterium leprae (286 aa); and highly similar to others from Mycobacteria e.g. upstream ORF P72028|mmaA4|Rv0642c|MTCY20H10.23c putative methoxy mycolic acid synthase 4 from Mycobacterium tuberculosis (301 aa). P9WPB1 No lipid metabolism P0C5C3 P9WPB1 GO:0008610,GO:0008825 2.1.1.- P9WPB1 NC_000962.3 Mycobrowser_v5 CDS 742719 746366 . + 0 Rv0648 Rv0648 Alpha-mannosidase activity: hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. Alpha-mannosidase Rv0648, (MTCY20H10.29), len: 1215 aa. Alpha-mannosidase (see citation below), showing some similarity to hypothetical proteins and various sugar hydrolases e.g. SYCSLRA_6|Q55528 hypothetical 1 20.4 kDa protein from Synechocystis (1042 aa), FASTA scores: opt: 260, E(): 3.6e-08, (23.4% identity in 602 aa overlap); etc. Contains PS00659 Glycosyl hydrolases family 5 signature. P96937 No intermediary metabolism and respiration P96937 P96937 GO:0030246,GO:0006013,GO:0008270,GO:0004559 3.2.1.- P96937 NC_000962.3 Mycobrowser_v5 CDS 786149 786946 . + 0 Rv0686 Rv0686 Unknown Probable membrane protein Rv0686, (MTCY210.03), len: 265 aa. Probable membrane protein, with hydrophobic N-terminus. I6XVZ6 No cell wall and cell processes I6XVZ6 NC_000962.3 Mycobrowser_v5 CDS 749234 749929 . - 0 Rv0653c Rv0653c Involved in transcriptional mechanism. Possible transcriptional regulatory protein (probably TetR-family) Rv0653c, (MTCI376.23, MTCY20H10.34c), len: 231 aa. Possible transcriptional regulator, TetR family, similar in N-terminus to others e.g. CAC03642.1|AL391338 putative TetR-family transcriptional regulator from Streptomyces coelicolor (190 aa); Q51597 cam repressor from Pseudomonas putida (186 aa), FASTA scores: opt: 150, E(): 0.00085, (27.8% identity in 97 aa overlap); etc. Also some similarity to Mycobacterium tuberculosis hypothetical transcriptional regulators Rv0681 and Rv1816. Contains probable helix-turn helix motif from aa 27-48 (Score 1156, +3.12 SD). P96941 No regulatory proteins P96941 P96941 GO:0006350,GO:0003700,GO:0006355 P96941 NC_000962.3 Mycobrowser_v5 CDS 750000 751505 . + 0 Rv0654 Rv0654 Function unknown; probably involved in cellular metabolism. Probable dioxygenase Rv0654, (MTCI376.22), len: 501 aa. Probable dioxygenase, highly similar to others eg AAK06796.1|AF324838_15|AF324838|SimC5 putative dioxygenase (involved in tetraene formation) from Streptomyces antibioticus (456 aa); CAB56138.1| AL117669 putative dioxygenase from Streptomyces coelicolor (503 aa); T51734 neoxanthin cleavage enzyme (9-cis-epoxy-carotenoid dioxygenase) from Arabidopsis thaliana (538 aa); Q53353 lignostilbene-alpha,beta-dioxygenase from Pseudomonas paucimobilis (Sphingomonas paucimobilis), FASTA scores: opt: 280, E(): 2.3e-11, (28.5% identity in 523 aa overlap); etc. Also some similarity with Rv0913c|MTCY21C12.07c possible dioxygenase from Mycobacterium tuberculosis (501 aa), FASTA score: (29.5% identity in 522 aa overlap). P9WPR5 No intermediary metabolism and respiration O06785 P9WPR5 GO:0016702,GO:0055114 1.-.-.- NC_000962.3 Mycobrowser_v5 CDS 753693 754409 . - 0 Rv0658c Rv0658c Unknown. Supposedly involved in stationary-phase survival. Probable conserved integral membrane protein Rv0658c, (MTCI376.18), len: 238 aa. Probable conserved integral membrane protein, equivalent to a predicted homologous protein from Mycobacterium smegmatis (see citation below), and showing some similarity with P33774|YPRB_ECOLI hypothetical 24.3 kDa protein from Escherichia coli (217 aa), FASTA scores: opt: 174, E(): 5.3e-05, (25.6% identity in 223 aa overlap). Also similar to Rv1863c and Rv0804 from Mycobacterium tuberculosis. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06781 No cell wall and cell processes O06781 O06781 GO:0016020 NC_000962.3 Mycobrowser_v5 CDS 756137 758500 . + 0 Rv0663 atsD Thought to play an important role in the mineralization of sulfates [catalytic activity: a phenol sulfate + H2O = a phenol + sulfate]. Possible arylsulfatase AtsD (aryl-sulfate sulphohydrolase) (arylsulphatase) Rv0663, (MTCI376.13c), len: 787 aa. Possible atsD, arylsulfatase, similar to others e.g. P5169|ARS_PSEAE arylsulfatase from Pseudomonas aeruginosa (532 aa), FASTA scores: opt: 653, E(): 0, (33.1% identity in 544 aa overlap); etc. Also similar to P95059|MTCY210.30|ATSA|Rv0711|MTCY210.30 from Mycobacterium tuberculosis (787 aa), FASTA score: (38.9% identity in 769 aa overlap); and other arylsulfatases from Mycobacterium tuberculosis e.g. Rv3299c|ATSB (970 aa), Rv0711, etc. Contains PS00523 Sulfatases signature 1. Belongs to the sulfatase family. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6XVW9 No intermediary metabolism and respiration O06776 I6XVW9 GO:0008152,GO:0004065 3.1.6.1 I6XVW9 NC_000962.3 Mycobrowser_v5 CDS 787099 787926 . + 0 Rv0687 Rv0687 Function unknown; possibly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv0687, (MTCY210.04), len: 275 aa. Probable short-chain dehydrogenase/reductase, highly similar to various dehydrogenases (generally SDR family) e.g. U17129|RSU17129_7 short-chain dehydrogenase from Rhodococcus erythropolis (275 aa), FASTA scores: opt: 1112, E(): 0, (61.2% identity in 268 aa overlap); MMU34072_2 steroid dehydrogenase from Musmus culus (260 aa), FASTA scores: opt: 390, E(): 2.2e-17, (34.1% identity in 267 aa overlap); etc. Also similar to MTV002_16|O33292|Rv2750 dehydrogenase from Mycobacterium tuberculosis (272 aa). Contains PS00061 Short-chain alcohol dehydrogenase family signature. P9WGS7 No intermediary metabolism and respiration P95033 P9WGS7 GO:0055114,GO:0016491 1.-.-.- P9WGS7 NC_000962.3 Mycobrowser_v5 CDS 763370 767320 . + 0 Rv0668 rpoC Catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [catalytic activity: N nucleoside triphosphate = N diphosphate + {RNA}(N)]. DNA-directed RNA polymerase (beta' chain) RpoC (transcriptase beta' chain) (RNA polymerase beta' subunit). Rv0668, (MTCI376.07c), len: 1316 aa. RpoC, DNA-directed RNA polymerase, beta' chain (see Miller et al., 1994), equivalent to P30761|RPOC_MYCLE|ML1890|S31146 DNA-directed RNA polymerase beta' chain from Mycobacterium leprae (1316 aa), FASTA scores: opt: 8295, E(): 0, (95.6% identity in 1316 aa overlap). Also highly similar to others e.g. CAB77429.1|AL160431 DNA-directed RNA polymerase beta' chain (fragment) from Streptomyces coelicolor (1059 aa); P37871|RPOC_BACSU from Bacillus subtilis (1199 aa), FASTA scores: opt: 2367, E(): 0, (52.9 identity in 1317 aa overlap); etc. Belongs to the RNA polymerase beta' chain family. P9WGY7 No information pathways P0A674 P9WGY7 GO:0003899,GO:0006351,GO:0003677 2.7.7.6 P9WGY7 NC_000962.3 Mycobrowser_v5 CDS 767684 769597 . - 0 Rv0669c Rv0669c Function unknown; hydrolytic enzyme probably involved in cellular metabolism. Possible hydrolase Rv0669c, (MTCI376.05), len: 637 aa. Possible hydrolase, highly similar to various hydrolases (N-terminus shorter) e.g. BAA88409.1|AB028646 alkaline ceramidase from Pseudomonas aeruginosa (670 aa,) FASTA scores: opt: 1490, E(): 0, (41.2% identity in 651 aa overlap); NP_063946.1|NM_019893 mitochondrial ceramidase from Homo sapiens (761 aa); P_446098.1|NM_053646 N-acylsphingosine amidohydrolase 2 from Rattus norvegicus (761 aa); BAB09641.1|AB016885 neutral ceramidase from Arabidopsis thaliana (705 aa); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). O06769 No intermediary metabolism and respiration O06769 GO:0016787 3.-.-.- O06769 NC_000962.3 Mycobrowser_v5 CDS 770582 771424 . + 0 Rv0671 lpqP Unknown Possible conserved lipoprotein LpqP Rv0671, (MTCI376.03c), len: 280 aa. Possible lpqP, conserved lipoprotein, similar to U00012|B1308_F2_43|Q49658 from Mycobacterium leprae (302 aa), FASTA scores: opt: 449, E(): 2.4e-22, (37.6% identity in 242 aa overlap). Also highly similar to lpqC|Rv3298c|MTCY71.38c putative lipoprotein from Mycobacterium tuberculosis (304 aa). Also similar to a large variety of proteins including various esterases and poly(3-hydroxyalkanoate) depolymerases, e.g. NP_249234.1|NC_002516 hypothetical protein from Pseudomonas aeruginosa (322 aa); C-terminus of AAD45376.1|AF164516_1|AF164516 cinnamoyl ester hydrolase EstA from Piromyces equi (536 aa); part of P52090|PHA1_PSELE poly(3-hydroxyalkanoate) depolymerase C precursor from Pseudomonas lemoignei (414 aa); CAC10310.1|AL442629 putative secreted protein from Streptomyces coelicolor (348 aa); etc. Has a 17 aa signal sequence and contains appropriately positioned (PS00013) Prokaryotic membrane lipoprotein lipid attachment site. I6XVY0 No cell wall and cell processes I6XVY0 GO:0016787 NC_000962.3 Mycobrowser_v5 CDS 771484 773112 . + 0 Rv0672 fadE8 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE8 Rv0672, (MTCI376.02c), len: 542 aa. Probable fadE8, acyl-CoA dehydrogenase, highly similar to many e.g. CAC33951.1|AL589708 putative acyl-CoA dehydrogenase from Streptomyces coelicolor (557 aa); P33224|AIDB_ECOLI|B4187 aidb protein (acyl-CoA dehydrogenases family) from Escherichia coli strain K12 (546 aa), FASTA scores: opt: 1369, E(): 0, (44.1% identity in 524 aa overlap); etc. Also similar to several other M. tuberculosis proteins e.g. Rv0154c|MTCI5.28c FASTA score: (26.3% identity in 342 aa overlap); etc. Contains acyl-CoA dehydrogenases signature 2 (PS00073). Belongs to the acyl-CoA dehydrogenases family. I6X9J0 No lipid metabolism O86368 I6X9J0 GO:0055114,GO:0003995 1.3.99.- I6X9J0 NC_000962.3 Mycobrowser_v5 CDS 774783 775574 . + 0 Rv0675 echA5 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Probable enoyl-CoA hydratase EchA5 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv0675, (MTV040.03), len: 263 aa. Probable echA5, enoyl-CoA hydratase, similar to several e.g. NP_252116.1|NC_002516 probable enoyl CoA-hydratase/isomerase from Pseudomonas aeruginosa (256 aa); Q20376 protein similar to enoyl-CoA hydratase from Caenorhabditis elegans (258 aa), FASTA scores: opt: 697, E(): 0, (47.3% identity in 245 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. Z92669|MTCY8D5_17 (262 aa), FASTA scores: opt: 493, E(): 3.6e-25, (39.1% identity in 243 aa overlap); etc. I6Y4E8 No lipid metabolism Q7D9G0 I6Y4E8 GO:0016853,GO:0008152,GO:0004300 4.2.1.17 I6Y4E8 NC_000962.3 Mycobrowser_v5 CDS 781560 781934 . + 0 Rv0682 rpsL Protein S12 is involved in the translation initiation step. 30S ribosomal protein S12 RpsL Rv0682, (MTV040.10), len: 124 aa. rpsL, 30S ribosomal protein S12 (see citations below), equivalent to others from Mycobacteria e.g. P41195|RS12_MYCSM 30S ribosomal protein S12 from Mycobacterium smegmatis (124 aa); P51999|RS12_MYCAV 30S ribosomal protein S12 from Mycobacterium avium (124 aa); etc. Also highly similar to others from other organisms e.g. P97222|RS12_STRCO 30S ribosomal protein S12 from Streptomyces roseosporus, lividans and coelicolor (123 aa); etc. Contains PS00055 Ribosomal protein S12 signature. Belongs to the S12P family of ribosomal proteins. Nucleotide position 781922 in the genome sequence has been corrected, A:G resulting in K121K. P9WH63 No information pathways P41196 P9WH63 GO:0046677,GO:0015935,GO:0003735,GO:0000049,GO:0006412,GO:0019843 SM P9WH63 NC_000962.3 Mycobrowser_v5 CDS 787940 789160 . + 0 Rv0688 Rv0688 Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Putative ferredoxin reductase Rv0688, (MTCY210.05), len: 406 aa. Putative ferredoxin reductase, highly similar to others e.g. BAB55881.1|AB054975 ferredoxin reductase from Terrabacter sp. DBF63 (410 aa); CAC04223.1|AL391515 putative ferredoxin reductase from Streptomyces coelicolor (420 aa); PPU24215_8|Q51973 P-cumate dioxygenase ferredoxin reductase subunit from Pseudomonas putida (402 aa), FASTA scores: opt: 738, E(): 0, (38.8% identity in 330 aa overlap); etc. Also similar to Rv0253 and Rv1869c from Mycobacterium tuberculosis. Could belong to the bacterial type ferredoxin family. P95034 No intermediary metabolism and respiration P95034 P95034 GO:0045454,GO:0005737,GO:0055114,GO:0016491,GO:0050660 1.-.-.- P95034 NC_000962.3 Mycobrowser_v5 CDS 793335 794525 . + 0 Rv0694 lldD1 Involved in respiration; catalyzes conversion of lactate into pyruvate [catalytic activity: (S)-lactate + 2 ferricytochrome C = pyruvate + 2 ferrocytochrome C]. Possible L-lactate dehydrogenase (cytochrome) LldD1 Rv0694, (MTCY210.11), len: 396 aa. Possible lldD1, L-lactate dehydrogenase (cytochrome), similar to NP_302368.1|NC_002677 L-lactate dehydrogenase from Mycobacterium leprae (414 aa). Also similar to others e.g. NP_384560.1|NC_003047 putative L-lactate dehydrogenase (cytochrome) protein from Sinorhizobium meliloti (403 aa); NP_251072.1|NC_002516 L-lactate dehydrogenase from Pseudomonas aeruginosa (383 aa); P33232|LLDD_ECOLI L-lactate dehydrogenase (cytochrome) from Escherichia coli strain K12 (396 aa), FASTA scores: opt: 697, E(): 0, (34.5 identity in 380 aa overlap); etc; and also similar to other oxidoreductases. Note that also highly similar to RSU17129_5|AAC77479.1|U17129 unknown protein from Rhodococcus erythropolis (392 aa), FASTA scores: opt: 2006, E(): 0, (74.1% identity in 386 aa overlap). Also similar to lldD2|Rv1872c|MTCY180.46|MTCY359.01 possible L-lactate dehydrogenase (cytochrome) from Mycobacterium tuberculosis (414 aa). Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenases family. P9WND7 No intermediary metabolism and respiration P95040 P9WND7 GO:0004460,GO:0055114,GO:0010181 1.1.2.3 P9WND7 NC_000962.3 Mycobrowser_v5 CDS 795519 796931 . + 0 Rv0696 Rv0696 Function unknown; probably involved in cellular metabolism. Probable membrane sugar transferase Rv0696, (MTCY210.13), len: 470 aa. Probable membrane sugar transferase, similar (except in N-terminus) to NP_069157.1|NC_000917 glycosyl transferase from Archaeoglobus fulgidus (324 aa); NP_279985.1|NC_002607 rhamnosyl transferase from Halobacterium sp. NRC-1 (299 aa); NP_059113.1|NM_017417 polypeptide N-acetylgalactosaminyltransferase 8 from (637 aa). Note that also highly similar to P46370|YTH1_RHOER hypothetical 55.3 KDA protein from Rhodococcus erythropolis (513 aa), FASTA scores: opt: 1514, E(): 0, (51.8% identity in 469 aa overlap). P9WMX1 No intermediary metabolism and respiration P95042 P9WMX1 GO:0016740 2.-.-.- P9WMX1 NC_000962.3 Mycobrowser_v5 CDS 803411 803692 . + 0 Rv0705 rpsS Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA. 30S ribosomal protein S19 RpsS Rv0705, (MTCY210.24), len: 93 aa. rpsS, 30S ribosomal protein S19, equivalent to S36895 ribosomal protein S19 from Mycobacterium bovis (93 aa), FASTA scores: opt: 623, E(): 0, (98.9% identity in 93 aa overlap); and NP_302261.1|NC_002677 30S ribosomal protein S19 from Mycobacterium leprae (93 aa). Also highly similar to others e.g. CAB82074.1|AL161803 30S ribosomal protein S19 from Streptomyces coelicolor (93 aa); etc. Contains PS00323 Ribosomal protein S19 signature. Belongs to the S19P family of ribosomal proteins. P9WH45 No information pathways P0A5X4 P9WH45 GO:0015935,GO:0003735,GO:0006412,GO:0019843 P9WH45 NC_000962.3 Mycobrowser_v5 CDS 803689 804282 . + 0 Rv0706 rplV This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, E.G., L4, L17, and L20. It is important during the early stages of 50S reconstitution. 50S ribosomal protein L22 RplV Rv0706, (MTCY210.25), len: 197 aa. rplV, 50S ribosomal protein L22, equivalent to O06115|RL22_MYCSM 50S ribosomal protein L22 from Mycobacterium smegmatis (153 aa); MBS10OPER_7 50S ribosomal protein L22 from Mycobacterium bovis BCG; and MLCB2492_7 50S ribosomal protein L22 from Mycobacterium leprae (175 aa). Also highly similar to others e.g. CAB82075.1|AL161803 50S ribosomal protein L22 from Streptomyces coelicolor (125 aa); P42060|RL22_BACSU 50s ribosomal protein L22 from Bacillus subtilis (113 aa), FASTA scores: opt: 368, E(): 2.4e-13, (52.8% identity in 108 aa overlap); etc. Contains PS00464 Ribosomal protein L22 signature, and contains repetitive sequence at C-terminus. Belongs to the L22P family of ribosomal proteins. P9WHC1 No information pathways P61181 P9WHC1 GO:0019843,GO:0003735,GO:0006412,GO:0015934 P9WHC1 NC_000962.3 Mycobrowser_v5 CDS 806335 808698 . + 0 Rv0711 atsA Thought to play an important role in the mineralization of sulfates [catalytic activity: a phenol sulfate + H2O = a phenol + sulfate]. Possible arylsulfatase AtsA (aryl-sulfate sulphohydrolase) (arylsulphatase) Rv0711, (MTCY210.30), len: 787 aa. Possible atsA, arylsulfatase, similar to others e.g. P51691|ARS_PSEAE arylsulfatase from Pseudomonas aeruginosa (532 aa), FASTA scores: opt: 439, E(): 2.9e-21, (30.8% identity in 552 aa overlap); etc. Also similar to other hypothetical arylsulfatases from Mycobacterium tuberculosis e.g. Rv3299c, Rv0663, etc. Contains PS00523 Sulfatases signature 1, and PS00149 Sulfatases signature 2. Belongs to the sulfatase family. P95059 No intermediary metabolism and respiration P95059 P95059 GO:0008152,GO:0004065 3.1.6.1 P95059 NC_000962.3 Mycobrowser_v5 CDS 812976 813374 . + 0 Rv0718 rpsH Binds directly to the central domain of 16S ribosomal RNA. 30S ribosomal protein S8 RpsH Rv0718, (MTCY210.37), len: 132 aa. rpsH, 30S ribosomal protein S8, equivalent to O32997|MLCB2492_18 30S ribosomal protein S8 from Mycobacterium leprae (132 aa). Also highly similar to others e.g. CAB82084.1|AL161803 30S ribosomal protein S8 from Streptomyces coelicolor (132 aa); P33106|RS8_MICLU 30s ribosomal protein S8 from Micrococcus luteus (132 aa), FASTA scores: opt: 669, E(): 0, (77.3% identity in 132 aa overlap); etc. Contains PS00053 Ribosomal protein S8 signature. Belongs to the S8P family of ribosomal proteins. P9WH27 No information pathways P66625 P9WH27 GO:0019843,GO:0005840,GO:0003735,GO:0006412 P9WH27 NC_000962.3 Mycobrowser_v5 CDS 822866 823594 . + 0 Rv0730 Rv0730 Acetylation, substrate unknown GCN5-related N-acetyltransferase Rv0730, (MTV041.04), len: 242 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain in C-terminal part. See Vetting et al. 2005. Equivalent to Z98756|MLCB2492_26 hypothetical protein from Mycobacterium leprae (227 aa), FASTA scores: opt: 1180, E(): 0, (83.5% identity in 218 aa overlap). I6XW38 No intermediary metabolism and respiration I6XW38 GO:0008152,GO:0008080 I6XW38 NC_000962.3 Mycobrowser_v5 CDS 814328 814990 . + 0 Rv0721 rpsE Protein S5 is important in the assembly and function of the 30S ribosomal subunit. 30S ribosomal protein S5 RpsE Rv0721, (MTCY210.40), len: 220 aa. rpsE, 30S ribosomal protein S5, equivalent to MLCB2492_21 ribosomal protein S5 from Mycobacterium leprae (217 aa). Also highly similar to others e.g. P46790|RS5_STRCO 30s ribosomal protein S5 from Streptomyces coelicolor (167 aa), FASTA scores: opt: 889, E(): 0, (82.1% identity in 162 aa overlap); etc. Note N-terminus is extented compared to other rpsE genes. Contains PS00585 Ribosomal protein S5 signature, PTS HPr component phosphorylation sites signature. Belongs to the S5P family of ribosomal proteins. P9WH33 No information pathways P66574 P9WH33 GO:0015935,GO:0003735,GO:0006412,GO:0019843 P9WH33 NC_000962.3 Mycobrowser_v5 CDS 815663 817534 . + 0 Rv0724 sppA Involved in digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane. Possible protease IV SppA (endopeptidase IV) (signal peptide peptidase) Rv0724, (MTCY210.43), len: 623 aa. Possible sppA, protease IV (endopeptidase IV), equivalent (but longer 23 aa) to MLCB2492_24|O33003 endopeptidase IV from Mycobacterium leprae (602 aa). Also similar to others e.g. NP_419743.1|NC_002696 signal peptide peptidase SppA from Caulobacter crescentus (594 aa); P08395|SPPA_ECOLI|B1766 protease IV (endopeptidase) from Escherichia coli strain K-12 (618 aa), FASTA scores: opt: 582, E(): 8.9e-27, (34.1% identity in 525 aa overlap); etc. Belongs to peptidase family S49. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P95072 No cell wall and cell processes P95072 P95072 GO:0008233,GO:0006508,GO:0006465,GO:0016021 3.4.21.- P95072 NC_000962.3 Mycobrowser_v5 CDS 821507 822853 . + 0 Rv0729 xylB Phosphorylates D-xylulose [catalytic activity: ATP + D-xylulose = ADP + D-xylulose 5-phosphate]. Possible D-xylulose kinase XylB (xylulokinase) (xylulose kinase) Rv0729, (MTV041.03), len: 448 aa. Possible xylB, D-xylulose-kinase (xylulokinase). C-terminus highly similar to AAD09880.1|U77912 unknown protein from Mycobacterium bovis (102 aa); and N-terminus highly similar to T45387|Z98756|MLCB2492_25 hypothetical protein from Mycobacterium leprae (110 aa), FASTA scores: opt: 427, E(): 1.1e-19, (60.9% identity in 110 aa overlap). Also similar to xylA/xylB genes from various bacterial species e.g. AAC26499.1|AF045245 D-xylulose-kinase from Klebsiella pneumoniae (487 aa); NP_418021.1|NC_000913 xylulokinase from Escherichia coli strain K12 (484 aa), FASTA scores: opt: 260, E(): 7.5e-09, (25.9% identity in 478 aa overlap); etc. Also similar to Rv3696c|glpK probable glycerol kinase from Mycobacterium tuberculosis (517 aa). Belongs to the fucokinase / gluconokinase / glycerokinase / xylulokinase family. I6Y4K0 No intermediary metabolism and respiration O53793 I6Y4K0 GO:0004856,GO:0005975 2.7.1.17 I6Y4K0 NC_000962.3 Mycobrowser_v5 CDS 824800 826125 . + 0 Rv0732 secY Essential for protein export. Interacts with SECA|Rv3240c and SECE|Rv0638 to allow the translocation of proteins across the plasma membrane, by forming part of a channel. Probable preprotein translocase SecY Rv0732, (MTV041.06), len: 441 aa. Probable SecY, preprotein translocase (integral membrane protein) (see citation below), equivalent to NP_302243.1|NC_002677 SecY subunit of preprotein translocase from Mycobacterium leprae (438 aa); AAC04389.1|AF047021 preprotein translocase subunit from Mycobacterium smegmatis (438 aa); and U77912|MBU77912_1 preprotein translocase subunit from Mycobacterium bovis (441 aa), FASTA scores: opt: 2802, E(): 0, (99.8% identity in 441 aa overlap). Also highly similar to others e.g. P46785|SECY_STRCO preprotein translocase SECY subunit from Streptomyces coelicolor (437 aa); etc. Contains PS00755 and PS00756 protein secY signatures 1 and 2. Belongs to the SECE/SEC61-alpha family. Part of the prokaryotic protein translocation apparatus which comprise SECA|Rv3240c, SECD|Rv2587c, SECE|Rv0638, SECF|Rv2586c, SECG|Rv1440 and SECY. P9WGN3 No cell wall and cell processes P0A5Z2 P9WGN3 GO:0016021,GO:0005886,GO:0015031,GO:0055085,GO:0015450 NC_000962.3 Mycobrowser_v5 CDS 831776 832303 . + 0 Rv0740 Rv0740 Function unknown Conserved hypothetical protein Rv0740, (MTV041.14), len: 175 aa. Conserved hypothetical protein; C-terminus (possibly part of truncated IS1557) shows nearly perfect identity to Rv0750|MTV041_24 (81 aa), FASTA score: (92.6% identity in 81 aa overlap). Also shows weak similarity to MTV007_5 hypothetical protein from Mycobacterium tuberculosis (313 aa), FASTA score: (34.5% identity in 110 aa overlap); and MLCL536_27 hypothetical protein from Mycobacterium leprae (315 aa), FASTA score: (34.5% identity in 84 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53803 No conserved hypotheticals O53803 NC_000962.3 Mycobrowser_v5 CDS 834440 834946 . - 0 Rv0744c Rv0744c Thought to be involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0744c, (MTV041.18c), len: 168 aa. Possible transcriptional regulator, showing weak similarity with O86661|SC4A2.05 putative two-component sensor from Streptomyces coelicolor (436 aa), FASTA scores: opt: 117, E(): 0.88, (37.25% identity in 94 aa overlap); and some putative excisionases or transposases. Also weakly similar to P71902|YN10_MYCTU|Rv2310|MT2372|MTCY3G12.24c conserved hypothetical protein from Mycobacterium tuberculosis (114 aa); and Q11144|Y477_MYCTU|Rv0477|MT0495|MTCY20G9.03 conserved hypothetical protein from Mycobacterium tuberculosis (148 aa). Equivalent to AAK45006 from Mycobacterium tuberculosis strain CDC1551 (179 aa) but shorter 11 aa. Contains probable helix-turn helix motif from aa 5-26 (Score 1350, +3.78 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53807 No regulatory proteins O53807 GO:0000166,GO:0003677 NC_000962.3 Mycobrowser_v5 CDS 842347 843231 . - 0 Rv0751c mmsB Catalyzes the NAD-dependent, reversible oxidation of 3-hydroxbutyrate to methylmalonate [catalytic activity: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH]. Probable 3-hydroxyisobutyrate dehydrogenase MmsB (hibadh) Rv0751c, (MTV041.25c), len: 294 aa. Probable mmsB, 3-hydroxyisobutyrate dehydrogenase, highly similar to others e.g. NP_102847.1|NC_002678 3-hydroxyisobutyrate dehydrogenase from Mesorhizobium loti (294 aa); NP_420167.1|NC_002696 3-hydroxyisobutyrate dehydrogenase from Caulobacter crescentus (298 aa); A32867 3-hydroxyisobutyrate dehydrogenase from Rattus norvegicus (346 aa); etc. Also similar to methylmalonate semialdehyde dehydrogenases e.g. M84911|PSE MMSRAB_3 methylmalonate semialdehyde dehydrogenase from Pseudomonas aeruginosa (298 aa), FASTA scores: opt: 786, E(): 0, (45.8% identity in 297 aa overlap). Also similar to 6-phosphogluconate dehydrogenases from Mycobacterium tuberculosis e.g. Rv1122 and Rv1844c. Contains PS00895 3-hydroxyisobutyrate dehydrogenase signature. Belongs to the 3-hydroxyisobutyrate dehydrogenase family. P9WNY5 No intermediary metabolism and respiration P63935 P9WNY5 GO:0051287,GO:0055114,GO:0006098,GO:0004616,GO:0006573,GO:0008442 1.1.1.31 P9WNY5 NC_000962.3 Mycobrowser_v5 CDS 844421 845953 . - 0 Rv0753c mmsA Plays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA [catalytic activity: 2-methyl-3-oxopropanoate + CoA + NAD+ = propanoyl-CoA + CO2 + NADH]. Probable methylmalonate-semialdehyde dehydrogenase MmsA (methylmalonic acid semialdehyde dehydrogenase) (MMSDH) Rv0753c, (MTV041.27c), len: 510 aa. Probable mmsA, methylmalonic acid semialdehyde dehydrogenase, highly similar to others e.g. NP_420115.1|NC_002696 putative methylmalonate-semialdehyde dehydrogenase from Caulobacter crescentus (499 aa); L48550|STMMSDA_1|CAB75315.1|AL139164 methylmalonic acid semialdehyde dehydrogenase from Streptomyces coelicolor (500 aa), FASTA score: (51.6% identity in 498 aa overlap); M84911|PSEMMSRAB_2|NP_252260.1|NC_002516 methylmalonate-semialdehyde dehydrogenase from Pseudomonas aeruginosa (497 aa), FASTA scores: opt: 1127, E(): 0, (47.9% identity in 507 aa overlap); etc. Note that also highly similar to malonic semialdehyde oxidative decarboxylases e.g. NP_104968.1|NC_002678 malonic semialdehyde oxidative decarboxylase from Mesorhizobium loti (498 aa); NP_384832.1|NC_003047 putative malonic semialdehyde oxidative decarboxylase protein from Sinorhizobium meliloti (498 aa); etc. Contains PS00070 Aldehyde dehydrogenases cysteine active site. Belongs to the aldehyde dehydrogenases family. O53816 No intermediary metabolism and respiration O53816 O53816 GO:0055114,GO:0006573,GO:0004491 1.2.1.27 O53816 NC_000962.3 Mycobrowser_v5 CDS 851608 852351 . + 0 Rv0757 phoP Involved in transcriptional mechanism. Part of the two component regulatory system PHOP/PHOQ. This protein is thought to be a positive regulator for the phosphate regulon, required for intracellular growth. Transcription of this operon is positively regulated by PHOB and PHOR|Rv0758 when phosphate is limited. Possible two component system response transcriptional positive regulator PhoP Rv0757, (MTCY369.02), len: 247 aa. Possible phoP, two component system response phosphate regulon transcriptional regulator (see citations below), highly similar to various transcriptional regulators e.g. CAC32360.1|AL583945 putative two component system response regulator from Streptomyces coelicolor (271 aa); T45446 probable two-component response regulator from Mycobacterium leprae (253 aa); and similar to phoP proteins e.g. P13792|PHOP_BACSU alkaline phosphatase synthesis transcription regulatory protein from Bacillus subtilis (240 aa), FASTA scores: opt: 594, E(): 2.3e-33, (41.0% identity in 234 aa overlap); etc. Also highly similar to Rv3765c from Mycobacterium tuberculosis (234 aa), Rv1033c (257 aa), RV0903c|MTCY31.31c|Q10531 (236 aa), FASTA score: (45.4% identity in 229 aa overlap); MTCY10G2_16 and MTU88959_1. P71814 No regulatory proteins 2PMU,3R0J P71814 P71814 GO:0006355,GO:0000156,GO:0000160,GO:0003677 P71814 NC_000962.3 Mycobrowser_v5 CDS 852396 853853 . + 0 Rv0758 phoR Sensor part of a two component regulatory system. This protein is thought to be a sensor kinase for the phosphate regulon. Transcription of this operon is positively regulated by PHOB|Rv0757 and PHOR when phosphate is limited. Possible two component system response sensor kinase membrane associated PhoR Rv0758, (MTCY369.03), len: 485 aa. Possible phoR, two component system response phosphate sensor kinase membrane-associated, highly similar to various sensor kinases e.g. CAC32361.1|AL583945 putative two component system histidine kinase from Streptomyces coelicolor (524 aa); NP_349365.1|NC_003030 Membrane-associated sensory histidine kinase with HAMP domain from Clostridium acetobutylicum (482 aa); and similar to phoP proteins e.g. NP_372216.1|NC_002758 alkaline phosphatase synthesis sensor protein from Staphylococcus aureus (554 aa); P23545|PHOR_BACSU alkaline phosphatase synthesis sensor from Bacillus subtilis (579 aa), FASTA scores: opt: 515, E(): 1.9e-25, (40.0% identity in 230 aa overlap); etc. Also similar to proteins from Mycobacterium tuberculosis e.g. MTCY20G9.16 FASTA scores: (34.5% identity in 264 aa overlap), MTU88959_2 (509 aa), MTCY10G2_17, etc. P71815 No regulatory proteins P71815 P71815 GO:0016021,GO:0018106,GO:0007165,GO:0000155,GO:0000160,GO:0005524 2.7.13.3 P71815 NC_000962.3 Mycobrowser_v5 CDS 902111 903694 . + 0 Rv0808 purF Involved in de novo purine biosynthesis (at the first step) [catalytic activity: 5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O]. Amidophosphoribosyltransferase PurF (glutamine phosphoribosylpyrophosphate amidotransferase) (ATASE) (gpatase) Rv0808, (MTCY07H7A.01c), len: 527 aa. PurF, amidophosphoribosyltransferase, equivalent to MLCB5_32|Q50028|PURF from Mycobacterium leprae (556 aa), FASTA scores: (91.3% identity in 518 aa overlap); and CAB96578.1|AJ278609 phosphoribosyl pyrophosphate amidotransferase from Mycobacterium smegmatis (511 aa)(see citation below). Also highly similar to others e.g. BAA89439.1|AB003158 amidophosphoribosyl transferase from Corynebacterium ammoniagenes (490 aa); P00497|PUR1_BACSU amidophosphoribosyltransferase precursor from Bacillus subtilis (476 aa), FASTA scores: opt: 1412, E(): 0, (46.2% identity in 470 aa overlap); etc. Contains PS00103 Purine/pyrimidine phosphoribosyl transferases signature. Belongs to the purine/pyrimidine phosphoribosyltransferase family. P9WHQ7 No intermediary metabolism and respiration P65829 P9WHQ7 GO:0004044,GO:0006541,GO:0046872,GO:0009116,GO:0009113,GO:0006164,GO:0051539 2.4.2.14 P9WHQ7 NC_000962.3 Mycobrowser_v5 CDS 854699 855826 . - 0 Rv0761c adhB Thought to catalyze the reversible oxidation of ethanol to acetaldehyde with the concomitant reduction of NAD. Probably acts on primary or secondary alcohols or hemiacetals [catalytic activity: an alcohol + NAD+ = an aldehyde or ketone + NADH]. Possible zinc-containing alcohol dehydrogenase NAD dependent AdhB Rv0761c, (MTCY369.06c), len: 375 aa. Possible adhB, zinc-containing alcohol dehydrogenase NAD-dependent, similar to others e.g. AAC15839.1|AF060871_4 hypothetical alcohol dehydrogenase from Rhodococcus rhodochrous (370 aa), FASTA scores: opt: 1234, E(): 0, (46.8% identity in 370 aa overlap); P80468|ADH2_STRCA alcohol dehydrogenase II from Struthio camelus (Ostrich) (379 aa); Q03505|ADH1_RABIT alcohol dehydrogenase alpha chain from Oryctolagus cuniculus (Rabbit) (374 aa), FASTA scores: opt: 872, E(): 0, (39.1% identity in 379 aa overlap); etc. Also similar to adhD alcohol dehydrogenase from Mycobacterium tuberculosis (368 aa). Contains PS00059 Zinc-containing alcohol dehydrogenases signature. Belongs to the zinc-containing alcohol dehydrogenase family. P9WQC7 No intermediary metabolism and respiration P71818 P9WQC7 GO:0005737,GO:0055114,GO:0008270,GO:0004022 1.1.1.1 P9WQC7 NC_000962.3 Mycobrowser_v5 CDS 856682 858037 . - 0 Rv0764c cyp51 Involved in sterol biosynthesis. Its precise biological substrate is not known. Catalyzes C14-demethylation of lanosterol, 24,25-dihydrolanosterol and obtusifoliol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol. Cytochrome P450 51 Cyp51 (CYPL1) (P450-L1A1) (sterol 14-alpha demethylase) (lanosterol 14-alpha demethylase) (P450-14DM) Rv0764c, (MT0788, MTCY369.09c), len: 451 aa. Cyp51, cytochrome P450 51 (sterol 14-alpha demethylase), similar to others e.g. Q16850|CP51_HUMAN cytochrome P450 51 (CYPL1) (P450L1) (sterol 14-alpha demethylase) (lanosterol 14-alpha demethylase) from Homo sapiens (509 aa), FASTA scores: opt: 848, E(): 0, (33.9% identity in 439 aa overlap); NP_172633.1|NC_003070 putative obtusifoliol 14-alpha demethylase from Arabidopsis thaliana (488 aa); P93596|CP51_WHEAT cytochrome P450 51 (CYPL1) (P450-L1A1) (obtusifoliol 14-alpha demethylase) from Triticum aestivum (453 aa); etc. Also similar to many other Mycobacterium tuberculosis cytochromes P450 e.g. Rv1394c, FASTA score: (22.5% identity in 444 aa overlap). Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. Belongs to the cytochrome P450 family. P9WPP9 No intermediary metabolism and respiration 1E9X,1EA1,1H5Z,1U13,1X8V,2BZ9,2CI0,2CIB,2VKU,2W09,2W0A,2W0B P0A512 P9WPP9 GO:0009055,GO:0020037,GO:0055114,GO:0008398,GO:0016126,GO:0005737 1.14.13.70 P9WPP9 NC_000962.3 Mycobrowser_v5 CDS 860912 862381 . + 0 Rv0768 aldA Oxidizes a variety of aldehydes [catalytic activity: an aldehyde + NAD+ + H2O = an acid + NADH]. Probable aldehyde dehydrogenase NAD dependent AldA (aldehyde dehydrogenase [NAD+]) Rv0768, (MTCY369.13), len: 489 aa. Probable aldA, NAD-dependent aldehyde dehydrogenase, highly similar to others e.g. AAL14238.1|AY052630 6-oxolauric acid dehydrogenase from Rhodococcus ruber (474 aa); NP_285450.1|NC_001264 aldehyde dehydrogenase from Deinococcus radiodurans (495 aa); NP_241405.1|NC_002570 NADP-dependent aldehyde dehydrogenase from Bacillus halodurans (498 aa); P42757|DHAB_ATRHO betaine-aldehyde dehydrogenase precursor from Atriplex hortensis (Mountain spinach) (502 aa), FASTA scores: opt: 1001, E(): 0, (35.6% identity in 486 aa overlap); etc. Also highly similar to Rv0223c aldehyde dehydrogenase from Mycobacterium tuberculosis (487 aa). Contains PS00687 Aldehyde dehydrogenases glutamic acid active site. Belongs to the aldehyde dehydrogenases family. I6X9R9 No intermediary metabolism and respiration P71823 I6X9R9 GO:0016491,GO:0055114 1.2.1.- I6X9R9 NC_000962.3 Mycobrowser_v5 CDS 862412 863158 . + 0 Rv0769 Rv0769 Function unknown; probably involved in cellular metabolism. Probable dehydrogenase/reductase Rv0769, (MTCY369.14), len: 248 aa. Probable dehydrogenase/reductase, similar to others, especially short-chain type dehydrogenases/reductases and 3-oxoacyl-(acyl-carrier protein) reductases e.g. NP_106890.1|NC_002678 probable short-chain type dehydrogenase/reductase from Mesorhizobium loti (374 aa); NP_243357.1|NC_002570 3-oxoacyl-(acyl-carrier protein) reductase from Bacillus halodurans (246 aa); P28643|FABG_CUPLA 3-oxoacyl-[acyl-carrier protein] reductase from Cuphea lanceolata (320 aa); P25529|HDHA_ECOLI 7-alpha-hydroxysteroid dehydrogenase from Escherichia coli (255 aa), FASTA scores: opt: 536, E(): 6.5e-27, (37.7% identity in 247 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. MTCY02B10.14, FASTA score: (33.7% identity in 249 aa overlap); etc. P9WGQ9 No intermediary metabolism and respiration P71824 P9WGQ9 GO:0055114,GO:0016491 1.-.-.- P9WGQ9 NC_000962.3 Mycobrowser_v5 CDS 864586 865854 . + 0 Rv0772 purD Involved in de novo purine biosynthesis (at the second step) [catalytic activity: ATP + 5-phosphoribosylamine + glycine = ADP + phosphate + 5'-phosphoribosylglycinamide]. Probable phosphoribosylamine--glycine ligase PurD (GARS) (glycinamide ribonucleotide synthetase) (phosphoribosylglycinamide synthetase) (5'-phosphoribosylglycinamide synthetase) Rv0772, (MTCY369.17), len: 422 aa. Probable purD, phosphoribosylamine--glycine ligase, equivalent to Q50144|PURD|PUR2_MYCLE|ML2235|MLCB5.08 phosphoribosylamine--glycine ligase from Mycobacterium leprae (422 aa), FASTA scores: opt: 2272, E(): 0, (81.8% identity in 422 aa overlap). Also highly similar to others e.g. CAB56348.1|AL118514 phosphoribosylamine-glycine ligase from Streptomyces coelicolor (416 aa); P1564|PUR2_ECOLI phosphoribosylamine--glycine ligase from Escherichia coli (429 aa), FASTA scores: opt: 1039, E(): 0, (42.7% identity in 431 aa overlap); etc. Belongs to the GarS family. P9WHM9 No intermediary metabolism and respiration P65893 P9WHM9 GO:0046872,GO:0004637,GO:0009113,GO:0006164,GO:0005524 6.3.4.13 P9WHM9 NC_000962.3 Mycobrowser_v5 CDS 996524 997705 . + 0 Rv0894 Rv0894 Thought to be involved in transcriptional mechanism. Possible transcriptional regulatory protein (possibly LuxR-family) Rv0894, (MTCY31.22), len: 393 aa. Possible regulatory protein, LuxR family, highly similar in part to NP_302202.1|NC_002677 possible transcriptional regulator from Mycobacterium leprae (1106 aa). Also similar to others e.g. CAB95788.1|AL359949 putative multi-domain regulatory protein from Streptomyces coelicolor (780 aa); NP_107293.1|NC_002678 transcriptional regulator from Mesorhizobium loti (903 aa); etc. Also similar to other regulatory proteins from Mycobacterium tuberculosis e.g. Rv2488c|MTV008_44 (1137 aa), FASTA score: (53.2% identity in 363 aa overlap); Rv1358|MTCY02B10_22 (1159 aa), FASTA score: (52.3% identity in 365 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WKP9 No regulatory proteins P9WKP9 GO:0005524 P9WKP9 NC_000962.3 Mycobrowser_v5 CDS 870008 871426 . + 0 Rv0777 purB Involved in de novo purine biosynthesis (at the eight step) [catalytic activity: 1-(5-phosphoribosyl)-4-(N-succino-carboxamide) -5-aminoimidazole = fumarate + 5'-phosphoribosyl-5-amino-4-imidazolecarboxamide (also catalyzes: N6-(1,2-dicarboxyethyl)AMP = fumarate + AMP)]. Probable adenylosuccinate lyase PurB (adenylosuccinase) (ASL) (ASASE) Rv0777, (MTCY369.21b), len: 472 aa. Probable purB, adenylosuccinate lyase, equivalent (but shorter 15 aa) to MLCB5.13|Z95151|g2076607|PURB adenylosuccinate lyase from Mycobacterium leprae (487 aa), FASTA scores: opt: 2640, E(): 0, (86.7% identity in 472 aa overlap). More similar to eukaryotic adenylosuccinate lyases than to prokaryotic adenylosuccinate lyases e.g. P54822|PUR8_MOUSE adenylosuccinate lyase from Mus musculus (484 aa), FASTA scores: opt: 762, E(): 0, (32.4% identity in 445 aa overlap); CAB99134.1|AL390188 putative adenylosuccino lyase (fragment) from Streptomyces coelicolor (362 aa); etc. Contains PS00163 Fumarate lyases signature. Belongs to the lyase 1 family, adenylossucinate lyase subfamily. I6XWA1 No intermediary metabolism and respiration P71832 I6XWA1 GO:0004018,GO:0009152,GO:0070626 4.3.2.2 I6XWA1 NC_000962.3 Mycobrowser_v5 CDS 873343 874236 . + 0 Rv0780 purC Involved in de novo purine biosynthesis (at the seventh step) [catalytic activity: ATP + 1-(5-phosphoribosyl)-4-carboxy-5-aminoimidazole + L-aspartate = ADP + phosphate + 1-(5-phosphoribosyl)-4-(N-succino-carboxamide)-5-aminoimidazole]. Phosphoribosylaminoimidazole- succinocarboxamide synthase PurC (SAICAR synthetase) Rv0780, (MTCY369.24), len: 297 aa. PurC, phosphoribosylaminoimidazole- succinocarboxamide synthase (see citations below), equivalent to MTU34957_1|PURC phosphoribosylaminoimidazole-succinocarboxamide synthase from Mycobacterium leprae (297 aa), FASTA scores: opt: 1986, E(): 0, (99.3% identity in 297 aa overlap). Also similar to others e.g. CAB56351.1|AL118514 phosphoribosylaminoimidazole-succinocarboxamide synthase from Streptomyces coelicolor (299 aa); etc. Contains PS01058 SAICAR synthetase signature 2. Belongs to the SAICAR synthetase family. P9WHN1 No intermediary metabolism and respiration P0A5T4 P9WHN1 GO:0004639,GO:0006164,GO:0005524 6.3.2.6 P9WHN1 NC_000962.3 Mycobrowser_v5 CDS 874233 874943 . + 0 Rv0781 ptrBa Cleaves peptide bonds on the C-terminal side of lysyl and argininyl residues [catalytic activity: hydrolysis of ARG-|-XAA and LYS-|-XAA bonds in oligopeptides, even when P1' residue is proline]. Probable protease II PtrBa [first part] (oligopeptidase B) Rv0781, (MTCY369.25), len: 236 aa. Probable ptrBa, first part of protease II, equivalent to N-terminus of NP_302455.1|NC_002677 protease II from Mycobacterium leprae (724 aa). Also highly similar to N-termini of many proteases II e.g. P24555|PTRB_ECOLI|TLP|B1845 protease II from Escherichia coli strains K12 and HB101 (707 aa), FASTA scores: opt: 204, E(): 7.4e-07, (29.6% identity in 230 aa overlap); etc. ORFs Rv0782 and Rv0781 appear to be a frameshifted homologues of protease II, but we can find no error in the cosmid sequence to account for this. Belongs to peptidase family S9A; also known as the prolyl oligopeptidase family. Note that previously known as ptrBb. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P71835 No intermediary metabolism and respiration P71835 P71835 GO:0004252,GO:0006508 3.4.21.83 P71835 NC_000962.3 Mycobrowser_v5 CDS 876818 878440 . - 0 Rv0783c emrB Translocase that confers resistance to substances of high hydrophobicity. Involved in transport of multidrug across the membrane (export): multidrug resistance by an export mechanism. Responsible for the translocation of the substrate across the membrane. Possible multidrug resistance integral membrane efflux protein EmrB Rv0783c, (MTCY369.27c), len: 540 aa. Possible emrB, integral membrane drug efflux protein, member of major facilitator superfamily (MFS), equivalent to AAL16083.1|AF421382_1|AF421382 EmrB efflux protein from Mycobacterium avium (538 aa). Also similar to other membrane proteins e.g. CAB61606.1|AL133210 putative export protein from Streptomyces coelicolor (496 aa); NP_108371.1|NC_002678 efflux pump protein FarB from Mesorhizobium loti (511 aa); P44927|EMRB_HAEINHI0897| multidrug resistance protein b homologue from Haemophilus influenzae (510 aa), FASTA scores: opt: 706, E(): 1.3e-36, (30.4% identity in 408 aa overlap); etc. Also similar to Rv2333c|MTCY3G12.01 from Mycobacterium tuberculosis (537 aa), FASTA score: (28.2% identity in 408 aa overlap); and Rv1410c|MTCY21B4.27c from Mycobacterium tuberculosis (518 aa), FASTA score: (26.8% identity in 496 aa overlap). Belongs to the major facilitator family; also known as the drug resistance translocase family. P9WG89 No cell wall and cell processes P71836 P9WG89 GO:0005886,GO:0055085,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 879340 881040 . + 0 Rv0785 Rv0785 Function unknown Conserved protein Rv0785, (MTCY369.29), len: 566 aa. Conserved protein, highly similar to other conserved hypothetical proteins e.g. NP_105777.1| NC_002678 hypothetical protein from Mesorhizobium loti (552 aa); SC5F8.14|CAB93742.1|AL357613 conserved hypothetical protein from Streptomyces coelicolor (557 aa); AE001863|AE001863_31 from Deinococcus radiodurans (554 aa), FASTA scores: opt: 2243, E(): 0, (61.1% identity in 550 aa overlap); YEF7_YEAST|P32614 hypothetical 50.8 kd protein (470 aa), FASTA scores: opt: 169, E(): 0.0014, (23.8% identity in 542 aa overlap); etc. Also similar to Rv1817|MTCY1A11.26c from Mycobacterium tuberculosis (487 aa), FASTA score: (26.7% identity in 587 aa overlap). And shows similarity with other dehydrogenases. P71838 No conserved hypotheticals P71838 P71838 GO:0016491,GO:0009055 P71838 NC_000962.3 Mycobrowser_v5 CDS 887137 888636 . - 0 Rv0794c Rv0794c Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv0794c, (MTV042.04c), len: 499 aa. Probable oxidoreductase, possibly dihydrolipoamide dehydrogenase or mercuric reductase. Highly similar to CAB62675.1|AL133422 probable oxidoreductase from Streptomyces coelicolor (477 aa); and similar to various oxidoreductases e.g. P08663|MERA_STAAU mercuric reductase (HG(II) reductase) from Staphylococcus aureus (547 aa); AAK70920.1|AC087551_19|AC087551 putative lipoamide dehydrogenase from Oryza sativa (563 aa); NP_437349.1|NC_003078 putative FAD-dependent pyridine nucleotide-disulphide oxidoreductase, similar to mercuric reductases protein from Sinorhizobium meliloti (473 aa); Q04829|DLDH_HALVO dihydrolipoamide dehydrogenase from Haloferax volcanii (475 aa); P08332|MERA_SHIFL mercuric reductase (564 aa), FASTA scores: opt: 522, E(): 3.7e-26, (31.7% identity in 467 aa overlap); P72740|DLDH_SYNY3|Q53395|LPDA|PDHD|SLR1096 dihydrolipoamide dehydrogenase from Synechocystis sp. strain PCC 6803 (474 aa), FASTA scores: opt: 602, E(): 2.3e-31, (31.0% identity in 493 aa overlap); etc. Note that previously known as lpdB. I6Y4U4 No intermediary metabolism and respiration Q79FW1 I6Y4U4 GO:0045454,GO:0005737,GO:0055114,GO:0016491,GO:0050660 1.-.-.- I6Y4U4 NC_000962.3 Mycobrowser_v5 CDS 889347 890333 . + 0 Rv0796 Rv0796 Required for the transposition of the insertion element IS6110. Putative transposase for insertion sequence element IS6110 Rv0796, (MTV042.06), len: 328 aa. Putative transposase for IS6110. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv0795 and Rv0796, the sequence UUUUAAAG (directly upstream of Rv0796) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 50 aa). P9WKH9 No insertion seqs and phages P0C5G8 P9WKH9 GO:0015074,GO:0006310,GO:0032196,GO:0003677 P9WKH9 NC_000962.3 Mycobrowser_v5 CDS 892266 893273 . - 0 Rv0799c Rv0799c Function unknown Conserved protein Rv0799c, (MTCY07H7A.10, MTCI429B.01), len: 335 aa. Conserved protein, similar to Q50021|U2266C from Mycobacterium leprae (146 aa), FASTA scores: opt: 147, E(): 0.0016, (33.3% identity in 117 aa overlap); Q50020|U2266B from Mycobacterium leprae (27 aa), FASTA scores: opt: 94, E(): 1.3, (56.5% identity in 23 aa overlap). Also highly similar to others e.g. CAC01593.1|AL391041 conserved hypothetical protein from Streptomyces coelicolor (316 aa); AF088897|AF088897_9 hypothetical protein from Zymomonas mobilis (322 aa), FASTA scores: opt: 1132, E(): 0, (56.1% identity in 303 aa overlap); P76536|ECAE000330_8 hypothetical protein from Escherichia coli strain K-12 (308 aa), FASTA scores: E(): 2.2e-30, (37.4% identity in 297 aa overlap); etc. Also similar to some tyrA proteins. Predicted to be an outer membrane protein (See Song et al., 2008). I6Y4U9 No conserved hypotheticals O07180 I6Y4U9 I6Y4U9 NC_000962.3 Mycobrowser_v5 CDS 895820 898084 . + 0 Rv0803 purL Involved in de novo purine biosynthesis (at the fourth step) [catalytic activity: ATP + 5'-phosphoribosylformylglycinamide + L-glutamine + H(2)O = ADP + phosphate + 5'-phosphoribosylformylglycinamidine + L-glutamate]. Phosphoribosylformylglycinamidine synthase II PurL (FGAM synthase II) Rv0803, (MTCY07H7A.06c), len: 754 aa. PurL, phosphoribosylformylglycinamidine synthase II (see citations below), equivalent to NP_302451.1|NC_002677 phosphoribosylformylglycinamidine synthase II from Mycobacterium leprae (754 aa). Also highly similar to others e.g. Q9RKK5|PURL_STRCO from Streptomyces coelicolor (752 aa); P12042|PURL_BACSU from Bacillus subtilis (742 aa), FASTA score: (44.7% identity in 716 aa); etc. Start was chosen by similarity. Belongs to the FGAMS family. P9WHL7 No intermediary metabolism and respiration P0A5T8 P9WHL7 GO:0005524,GO:0005737,GO:0004642,GO:0006189 6.3.5.3 P9WHL7 NC_000962.3 Mycobrowser_v5 CDS 899732 901330 . - 0 Rv0806c cpsY Thought to be involved in exopolysaccharide and/or lipopolysaccharide biosynthetic pathway [catalytic activity: UDP-glucose = UDP-galactose]. Possible UDP-glucose-4-epimerase CpsY (galactowaldenase) (UDP-galactose-4-epimerase) (uridine diphosphate galactose-4-epimerase) (uridine diphospho-galactose-4-epimerase) Rv0806c, (MTCY07H7A.03), len: 532 aa. Possible cpsY, UDP-glucose-4-epimerase, equivalent to Q50025|CPSY probable UDP-glucose-4-epimerase from Mycobacterium leprae (542 aa), FASTA scores: opt: 2964, E(): 0, (82.3% identity in 530 aa overlap). Also similar to AAC38286.1|AF019760|SACB CpsY homolog (involved in meningococcal capsule biosynthesis) from Neisseria meningitidis serogroup a (545 aa); Q51151 capsule gene complex UPD-glucose-4-epimerase (gale) from Neisseria meningitidis (373 aa), FASTA scores: opt: 496, E(): 9.5e-27, (29.3% identity in 358 aa overlap); C-terminus of CAB75373.1|AL139298 putative transferase from Streptomyces coelicolor (942 aa); and many hypothetical proteins from Streptomyces coelicolor. Seems to belong to the sugar epimerase family. P9WGD1 No cell wall and cell processes P9WGD1 GO:0016740,GO:0000271 2.7.-.- NC_000962.3 Mycobrowser_v5 CDS 1024684 1025184 . + 0 Rv0919 Rv0919 Acetylation, substrate unknown GCN5-related N-acetyltransferase Rv0919, (MTCY21C12.13), len: 166 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Some similarity to Q50115 hypothetical protein from Mycobacterium leprae (90 aa), FASTA scores: opt: 243, E(): 5.3e-11, (56.5% identity in 85 aa overlap). Alternative nucleotide at position 1025106 (T->C; F141F) has been observed. I6XA42 No intermediary metabolism and respiration O05911 I6XA42 GO:0008152,GO:0008080 I6XA42 NC_000962.3 Mycobrowser_v5 CDS 908485 909318 . - 0 Rv0815c cysA2 May be a sulfotransferase involved in the formation of thiosulfate [catalytic activity: thiosulfate + cyanide = sulfite + thiocyanate]. Probable thiosulfate sulfurtransferase CysA2 (rhodanese-like protein) (thiosulfate cyanide transsulfurase) (thiosulfate thiotransferase) Rv0815c, (MTV043.07c, MT0837, O05793), len: 277 aa. Probable cysA2 (alternate gene name: sseC4), thiosulfate sulfurtransferase (see Wooff et al., 2002), equivalent to Q50036|CYSA|CYSA3|ML2198|THTR_MYCLE putative sulfurtransferase thiosulfate from Mycobacterium leprae (277 aa). Also highly similar to other putative thiosulfate sulfurtransferases e.g. P16385|THTR_SACER putative thiosulfate sulfurtransferase from Saccharopolyspora erythraea (Streptomyces erythraeus) (281 aa); NP_293941.1|NC_001263 thiosulfate sulfurtransferase from Deinococcus radiodurans (286 aa); etc. Identical second copy present as Rv3117|MTCY164.27|MT3199|O05793|cysA3 (277 aa) (100.0% identity in 277 aa overlap). Contains PS00683 Rhodanese C-terminal signature at C-terminus. Belongs to the rhodanese family. P9WHF9 No intermediary metabolism and respiration 3AAX,3AAY,3HWI O05793 P9WHF9 GO:0004792,GO:0008272 2.8.1.1 P9WHF9 NC_000962.3 Mycobrowser_v5 CDS 909611 910033 . - 0 Rv0816c thiX Thioredoxin participates in various redox reactions through the reversible oxidation of its active center dithiol, to a disulfide, & catalyzes dithiol-disulfide exchange reactions. Probable thioredoxin ThiX Rv0816c, (MTV043.08c), len: 140 aa. Probable thiX, thioredoxin, equivalent to ThiX|U15182|MLU15182_21 thioredoxin from Mycobacterium leprae (172 aa), FASTA scores: opt: 556, E(): 8.8e-31, (63.8% identity in 141 aa overlap); and similar to AAL08576.1|AF418548_2|AF418548 thioredoxin from Mycobacterium avium subsp. paratuberculosis (117 aa). Also similar to other bacterial thioredoxins e.g. CAB95303.1|AL359779 putative thioredoxin from Streptomyces coelicolor (126 aa); P33791|THIO_STRAU|TRX|TRXA thioredoxin from Streptomyces aureofaciens (106 aa); etc. And similar to Rv3914|MT4033|MTV028.05|NP_218431.1|NC_000962|trxC thioredoxin (TRX) (MPT46) from Mycobacterium tuberculosis (116 aa). Has hydrophobic stretch at N-terminus. Seems to belong to the thioredoxin family. I6Y8V2 No intermediary metabolism and respiration O53828 I6Y8V2 GO:0055114,GO:0016491,GO:0045454 1.-.-.- I6Y8V2 NC_000962.3 Mycobrowser_v5 CDS 913558 914199 . - 0 Rv0821c phoY2 Involved in transcriptional regulation of active transport of inorganic phosphate across the membrane. Probable phosphate-transport system transcriptional regulatory protein PhoY2 Rv0821c, (MTV043.13c), len: 213 aa. Probable phoY2, phosphate-transport system regulatory protein, highly similar to PhoY|MLU15182_29|U15182 phosphate transport system regulator from Mycobacterium leprae (222 aa), FASTA scores: opt: 1268, E(): 0, (93.0% identity in 213 aa overlap). Also similar to others e.g. NP_384620.1|NC_003047 probable phosphate transport system transcriptional regulator protein from Sinorhizobium meliloti (237 aa); etc. Also highly similar to MTCI418A.03c|Z96070|PhoY1 probable phosphate transport system transcriptional regulator protein from Mycobacterium tuberculosis (221 aa), FASTA scores: opt: 937, E(): 0, (63.4% identity in 213 aa overlap). Belongs to the PhoU family. P9WI95 No cell wall and cell processes P65720 P9WI95 GO:0006817,GO:0005737 P9WI95 NC_000962.3 Mycobrowser_v5 CDS 914257 916311 . - 0 Rv0822c Rv0822c Function unknown Conserved protein Rv0822c, (MTV043.14c), len: 684 aa. Conserved protein, highly similar in the region between aa 370 - 580 to U2266O|U15182|MLU15182_30 hypothetical protein from Mycobacterium leprae (222 aa), FASTA scores: opt: 819, E(): 0, (60.6% identity in 221 aa overlap). More extended similarity to Rv3267|Z92771|MTCY71_7 from Mycobacterium tuberculosis (498 aa), FASTA scores: opt: 434, E(): 2.2e-17, (26.6% identity in 541 aa overlap), and Rv3484. Also similar to various proteins, preferiously putative membrane proteins and membrane-bound regulatory proteins e.g. CAC44512.1|AL596138 putative membrane protein from Streptomyces coelicolor (524 aa); U56901|BSU56901_1 regulatory protein from Bacillus subtilis (391 aa), FASTA scores: opt: 225, E(): 1.3e-05, (24.7% identity in 340 aa overlap). Contains hydrophobic stretch (aa ~ 160-195) and PS00041 Bacterial regulatory proteins, araC family signature. I6WZI4 No conserved hypotheticals O53834 I6WZI4 I6WZI4 NC_000962.3 Mycobrowser_v5 CDS 925361 927610 . + 0 Rv0833 PE_PGRS13 Function unknown PE-PGRS family protein PE_PGRS13 Rv0833, (MTV043.25), len: 749 aa. PE_PGRS13, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), but lacking N-terminal domain (present in preceding ORF), possibly due to frameshift. Similar in part to many others e.g. MTCY28_25|Z95890 (914 aa), FASTA scores: opt: 2726, E(): 0, (60.1% identity in 776 aa overlap); etc. Q79FV7 No PE/PPE Q79FV7 NC_000962.3 Mycobrowser_v5 CDS 927837 930485 . - 0 Rv0834c PE_PGRS14 Function unknown PE-PGRS family protein PE_PGRS14 Rv0834c, (MTV043.26c), len: 882 aa. PE_PGRS14, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002), highly similar to many others e.g. MTCY493_4|Z95844 (1329 aa), FASTA scores: opt: 2577, E(): 0, (52.0% identity in 950 aa overlap); etc. Thought to be differentially expressed within host cells (see Triccas et al., 1999). Q79FV6 No PE/PPE Q79FV6 Q79FV6 Q79FV6 NC_000962.3 Mycobrowser_v5 CDS 930953 931597 . + 0 Rv0835 lpqQ Unknown Possible lipoprotein LpqQ Rv0835, (MTV043.27), len: 214 aa. Possible lpqQ, lipoprotein. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O53846 No cell wall and cell processes O53846 O53846 NC_000962.3 Mycobrowser_v5 CDS 932279 932932 . - 0 Rv0836c Rv0836c Unknown Hypothetical protein Rv0836c, (MTV043.29c), len: 217 aa (start uncertain). Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53848 No conserved hypotheticals O53848 O53848 NC_000962.3 Mycobrowser_v5 CDS 940456 941106 . - 0 Rv0844c narL Involved in transcriptional mechanism and regulates nitrate/nitrite. Possible nitrate/nitrite response transcriptional regulatory protein NarL Rv0844c, (MTV043.37c), len: 216 aa. Possible narL, nitrate/nitrite response regulator protein, similar to many e.g. CAB44989.1|AJ131854 NarL protein from Pseudomonas stutzeri (218 aa); CAA75536.1|Y15252 nitrate/nitrite regulatory protein from Pseudomonas aeruginosa (216 aa); PCC6803|D64005|SYCSLRG_24 NarL protein from Synechocystis sp. (209 aa), FASTA scores: opt: 438, E(): 1.5e-23, (34.6% identity in 208 aa overlap); etc. Also similar to unidentified regulator e.g. CAB76009.1|AL157916 putative two-component system response regulator from Streptomyces coelicolor (224 aa); etc. Contains probable helix-turn helix motif from aa 170-191 (Score 1124, +3.02 SD). P9WGM5 No regulatory proteins 3EUL O53856 P9WGM5 GO:0006355,GO:0043565,GO:0006350,GO:0003700,GO:0000156,GO:0000160,GO:0005622 P9WGM5 NC_000962.3 Mycobrowser_v5 CDS 941190 942467 . + 0 Rv0845 Rv0845 Possible sensor part of a two component regulatory system. Possible two component sensor kinase Rv0845, (MTV043.38), len: 425 aa. Possible two-component sensor kinase, with its C-terminus similar to C-terminal part of others e.g. NP_294951.1|NC_001263 two-component sensor histidine kinase from Deinococcus radiodurans (469 aa); CAC32293.1|AL583943 putative two component system histidine kinase from Streptomyces coelicolor (404 aa); NP_464546.1|NC_003210 protein similar to two-component sensor histidine kinase from Listeria monocytogenes (352 aa); BSUB0017_193|Z9912 two-component sensor kinase from Bacillus subtilis (360 aa), FASTA scores: opt: 275, E(): 1.6e-11, (30.3% identity in 234 aa overlap); etc. O53857 No regulatory proteins O53857 O53857 GO:0018106,GO:0007165,GO:0000155,GO:0005524 2.7.-.- O53857 NC_000962.3 Mycobrowser_v5 CDS 944938 946056 . + 0 Rv0848 cysK2 Thought to be involved in cysteine biosynthesis [catalytic activity: O3-acetyl-L-serine + H(2)S = L-cysteine + acetate]. Possible cysteine synthase a CysK2 (O-acetylserine sulfhydrylase) (O-acetylserine (thiol)-lyase) (CSASE) Rv0848, (MTV043.41), len: 372 aa. Possible cysK2, cysteine synthase A, but could be also a cysteine synthase B cysM2-product, similar to many e.g. NP_109408.1|NC_002682 cysteine synthase from Mesorhizobium loti (357 aa); Q44004|CYSM_ALCEU cysteine synthase from Alcaligenes eutrophus strain CH34 (Ralstonia eutropha) (339 aa), FASTA scores: opt: 511, E(): 1.7e-25, (35.0% identity in 314 aa overlap); etc. Belongs to the cysteine synthase/cystathionine beta-synthase family. Cofactor: pyridoxal phosphate. Note that previously known as cysM3. Q79FV4 No intermediary metabolism and respiration Q79FV4 Q79FV4 GO:0008152,GO:0030170,GO:0016740,GO:0004124 2.5.1.47 Q79FV4 NC_000962.3 Mycobrowser_v5 CDS 947641 948468 . - 0 Rv0851c Rv0851c Function unknown; possibly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv0851c, (MTV043.44c), len: 275 aa. Probable short-chain dehydrogenase/reductase, similar to many e.g. Q01198|LIGD_PSEPA C alpha-dehydrogenase (SDR family) from Pseudomonas paucimobilis (Sphingomonas paucimobilis) (305 aa); D11473|PSELIG_1 C alpha-dehydrogenase from P. paucimobilis (305 aa), FASTA scores: opt: 468, E(): 4.9e-23, (30.8% identity in 279 aa overlap); NP_421969.1|NC_002696 short chain dehydrogenase family protein from Caulobacter crescentus (278 aa); etc. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. O53863 No intermediary metabolism and respiration O53863 O53863 GO:0055114,GO:0016491 1.-.-.- O53863 NC_000962.3 Mycobrowser_v5 CDS 1025497 1026816 . - 0 Rv0920c Rv0920c Required for the transposition of the insertion element IS1554. Probable transposase Rv0920c, (MTCY21C12.14c), len: 439 aa. Probable transposase for IS1554, highly similar to others e.g. MTCY441.35|Q45111 transposase from Mycobacterium tuberculosis (419 aa), FASTA scores: opt: 1113, E(): 0, (43.9% identity in 378 aa overlap); etc. Contains transposases mutator family signature (PS01007). I6Y941 No insertion seqs and phages O05912 I6Y941 GO:0004803,GO:0006313,GO:0003677 I6Y941 NC_000962.3 Mycobrowser_v5 CDS 951632 952711 . + 0 Rv0855 far Function unknown, but involvement in lipid degradation (racemization). Probable fatty-acid-CoA racemase Far Rv0855, (MTV043.48), len: 359 aa. Probable far, fatty acid-CoA racemase, highly similar to CAB08122.1|Z94723 unknown protein from Mycobacterium leprae (253 aa) (C-terminus shorter). Also similar to many eukaryotic and bacteria racemases e.g. T35425 probable fatty acid CoA racemase from Streptomyces coelicolor (387 aa); P70473|AMAC_RAT alpha-methylacyl-CoA racemase (2-methylacyl-CoA racemase) (2-arylpropionyl-CoA epimerase) from Rattus norvegicus (Rat) (382 aa); NP_103687.1|NC_002678 probable fatty acid Co-a racemase from Mesorhizobium loti (389 aa); etc. Also similar to proteins from Mycobacterium tuberculosis e.g. Rv1143|MTCI65.10|MCR from Mycobacterium tuberculosis (360 aa), FASTA scores: opt: 1373, E(): 0, (56.8% identity in 359 aa overlap), Rv1866|MTCY359.07 (C-terminal half) (778 aa), Rv3272 (360 aa). I6Y8Y0 No lipid metabolism 2G04 O53867 I6Y8Y0 GO:0008152,GO:0016853 5.1.-.- I6Y8Y0 NC_000962.3 Mycobrowser_v5 CDS 955077 956288 . + 0 Rv0859 fadA Function unknown, but involvement in lipid degradation. Possible acyl-CoA thiolase FadA Rv0859, (MTV043.52), len: 403 aa. Possible fadA, acyl-CoA thiolase, equivalent to NP_302423.1|NC_002677 putative beta-ketoadipyl CoA thiolase from Mycobacterium leprae (403 aa). Also highly similar to acyl/acetyl-CoA thiolases and beta-ketoadipyl CoA thiolases, e.g. T35428 probable acetyl CoA acetyltransferase (thiolase) from Streptomyces coelicolor (404 aa); NP_250427.1|NC_002516 probable acyl-CoA thiolase from Pseudomonas aeruginosa (401 aa); NP_106253.1|NC_002678 probable acyl-CoA thiolase from Mesorhizobium loti (402 aa); NP_248919.1|NC_002516|PcaF beta-ketoadipyl CoA thiolase PcaF from Pseudomonas aeruginosa (401 aa); etc. Contains PS00098 Thiolases acyl-enzyme intermediate signature, PS00737 Thiolases signature 2 and PS00099 Thiolases active site. O53871 No lipid metabolism O53871 O53871 GO:0008152,GO:0008415 2.3.1.- O53871 NC_000962.3 Mycobrowser_v5 CDS 956293 958455 . + 0 Rv0860 fadB Involved in fatty acid degradation (probably in fatty acid beta-oxidation cycle). Probable fatty oxidation protein FadB Rv0860, (MTV043.53), len: 720 aa. Probable fadB, fatty oxidation protein, equivalent to NP_302422.1|NC_002677 putative fatty oxidation complex alpha subunit from Mycobacterium leprae (714 aa). Also highly similar to others and various proteins involved in fatty acid metabolism, e.g. T35429 probable fatty oxidation protein from Streptomyces coelicolor (733 aa); NP_250428.1|NC_002516 probable 3-hydroxyacyl-CoA dehydrogenase from Pseudomonas aeruginosa (714 aa); NP_418895.1|NC_002696 fatty oxidation complex alpha subunit from Caulobacter crescentus (709 aa); P40939|ECHA_HUMAN trifunctional enzyme alpha subunit [includes: long-chain enoyl-CoA hydratase ; long chain 3-hydroxyacyl-CoA dehydrogenase ] from Homo sapiens (763 aa), FASTA scores: opt: 1176, E(): 0, (32.4% identity in 722 aa overlap); P21177|FADB_ECOLI fatty oxidation complex alpha subunit [includes: enoyl-CoA hydratase; delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase; 3-hydroxyacyl-CoA dehydrogenase; 3- hydroxybutyryl-CoA epimerase] from Escherichia coli strain K12 (729 aa), FASTA scores: opt: 873, E(): 0, (33.6% identity in 693 aa overlap); etc. O53872 No lipid metabolism O53872 O53872 GO:0006631,GO:0016491,GO:0050662 O53872 NC_000962.3 Mycobrowser_v5 CDS 958523 960151 . - 0 Rv0861c ercc3 Involved in nucleotide excision repair. Has ATP-dependent 3' -> 5'helicase activity: acts by opening DNA either around the RNA transcription start site or the DNA damage. DNA helicase Ercc3 Rv0861c, (MTV043.54c), len: 542 aa. Ercc3, DNA helicase (see citation below), equivalent to NP_302420.1|NC_002677 probable DNA helicase from Mycobacterium leprae (549 aa). Also highly similar to others (shorter than several eukaryotic enzymes) e.g. NP_218820.1|NC_000919|AE001217|AE0 01217_6 putative DNA repair helicase from Treponema pallidum (606 aa), FASTA scores: opt: 1275, E(): 0, (47.5% identity in 592 aa overlap); Q00578|RA25_YEAST DNA repair helicase from Saccharomyces cerevisiae (843 aa), FASTA scores: opt: 777, E(): 0, (30.4% identity in 605 aa overlap); P49135|XPB_MOUSE DNA-repair protein complementing XP-B cells from Mus musculus (Mouse) (783 aa), FASTA scores: opt: 761, E(): 0, (36.3% identity in 375 aa overlap); etc. Seems to belong to the helicase family. Alternative nucleotide at position 958922 (C->a; A410A) has been observed. O53873 No information pathways O53873 O53873 GO:0004003,GO:0003677,GO:0006289,GO:0005524 3.6.1.- O53873 NC_000962.3 Mycobrowser_v5 CDS 962890 963393 . + 0 Rv0864 moaC2 Involved in the biosynthesis of molybdopterin. Probable molybdenum cofactor biosynthesis protein C 2 MoaC2 Rv0864, (MTV043.57), len: 167 aa. Probable moaC2, molybdopterin cofactor biosynthesis protein, highly similar to others e.g. CAB59676.1|AL132674 molybdenum cofactor biosynthesis protein from Streptomyces coelicolor (170 aa); NP_418834.1|NC_002696 molybdenum cofactor biosynthesis protein C from Caulobacter crescentus (186 aa); Y10817|ANY10817_3|T44852 molybdopterin co-factor synthesis protein moaC from Arthrobacter nicotinovorans plasmid pAO1 (169 aa), FASTA scores: opt: 491, E(): 2.4e-29, (51.0% identity in 151 aa overlap); etc. Also highly similar to O05788|MOAC1|Rv3111|MTCY164.21 putative molybdenum cofactor biosynthesis protein C from Mycobacterium tuberculosis (170 aa), FASTA scores: opt: 491, E(): 2.4e-29, (54.9% identity in 153 aa overlap); and O53376|Rv3324c|MOAC3|MTV016.24c putative molybdenum cofactor biosynthesis protein C3 (177 aa). P9WJR7 No intermediary metabolism and respiration P0A5K6 P9WJR7 GO:0006777 P9WJR7 NC_000962.3 Mycobrowser_v5 CDS 963390 963872 . + 0 Rv0865 mog Involved in molybdopterin biosynthesis; involved in the biosynthesis of a demolybdo-cofactor (molybdopterin), necessary for molybdo-enzymes. Probable molybdopterin biosynthesis Mog protein Rv0865, (MTV043.58), len: 160 aa. Probable mog, molybdopterin biosynthesis MOG protein, highly similar or similar to other molybdenum cofactor biosynthesis proteins e.g. CAB59675.1|AL132674 molybdenum cofactor biosynthesis protein from Streptomyces coelicolor (179 aa); NP_301253.1|NC_002677 putative molybdenum cofactor biosynthesis protein from Mycobacterium leprae (181 aa); CAC39235.1|AJ312124 Mog protein from Eubacterium acidaminophilum (162 aa); P44645|MOG_HAEIN|MOGA|HI0336 molybdopterin biosynthesis MOG protein from Haemophilus influenzae (197 aa), FASTA scores: opt: 306, E(): 9e-13, (39.6% identity in 139 aa overlap); P28694|MOG_ECOLI molybdopterin biosynthesis MOG protein from Escherichia coli (195 aa), FASTA scores: opt: 265, E(): 3.6e-10, (34.2 identity in 146 aa overlap); etc. Also highly similar to Rv0984|MTV044.12|MOAB2 possible pterin-4-alpha-carbinolamine dehydratase from Mycobacterium tuberculosis (181 aa). I6Y8Y8 No intermediary metabolism and respiration 2G4R O53877 I6Y8Y8 GO:0006777 I6Y8Y8 NC_000962.3 Mycobrowser_v5 CDS 963869 964294 . + 0 Rv0866 moaE2 Possibly a molybdenum biosynthesis cofactor. Conversion of molybdopterin precursor Z into molybdopterin requires transfer of two sulfur atoms to precursor Z (to generate the dithiolene group). This is catalyzed by the converting factor composed of a small and large subunit. Probable molybdenum cofactor biosynthesis protein E2 MoaE2 (molybdopterin converting factor large subunit) (molybdopterin [MPT] converting factor, subunit 2) Rv0866, (MTV043.59), len: 141 aa. Probable moaE2, molybdopterin converting factor E (molybdopterin converting factor (subunit 2)), similar to others e.g. Y10817|ANY10817_4|T44853 molybdopterin biosynthesis protein E chain from Arthrobacter nicotinovorans plasmid pAO1 (155 aa), FASTA scores: opt: 460, E(): 3.5e-27, (49.3 identity in 146 aa overlap); CAC01331.1|AL390968 moaE-like protein from Streptomyces coelicolor (152 aa); NP_389313.1|NC_000964 molybdopterin converting factor (subunit 2) from Bacillus subtilis (157 aa); etc. Also highly similar to Rv3119|MOAE1|Z95150|MTCY164_30 putative molybdenum cofactor biosynthesis protein E from Mycobacterium tuberculosis (147 aa), FASTA scores: opt: 321, E(): 5.9e-17, (40.9% identity in 132 aa overlap); and O53375|GPHA|Rv3323c|MTV016.23c MOAD-MOAE fusion protein from Mycobacterium tuberculosis (221 aa). P9WJR1 No intermediary metabolism and respiration O53878 P9WJR1 GO:0016740,GO:0006777 2.-.-.- P9WJR1 NC_000962.3 Mycobrowser_v5 CDS 966265 967347 . - 0 Rv0869c moaA2 Involved in molybdenum cofactor biosynthesis; involved in the biosynthesis of molybdopterin precursor Z from guanosine. Probable molybdenum cofactor biosynthesis protein A2 MoaA2 Rv0869c, (MTV043.62c), len: 360 aa. Probable moaA2, molybdenum cofactor biosynthesis protein, highly similar to others e.g. CAB59437.1|AL132644|SCI8_6 molybdenum cofactor biosynthesis protein A from Streptomyces coelicolor (341 aa), FASTA scores: opt: 1336, E(): 0, (61.7% identity in 332 aa overlap); S57490|X78980|ANMOAA_1 molybdopterin cofactor synthesis protein from Arthrobacter nicotinovorans (fragment) (374 aa), FASTA scores: opt: 1059, E(): 0, (49.9% identity in 369 aa overlap); Q44118|MOAA_ARTNI probable molybdopterin cofactor synthesis protein A from Arthrobacter nicotinovorans plasmid pAO1 (355 aa); etc. Also similar to Rv3109|MTCY164.19|Z95150|MOAA1 putative molybdenum cofactor biosynthesis protein A from Mycobacterium tuberculosis (359 aa), FASTA scores: opt: 657, E(): 0, (36.6% identity in 309 aa overlap). Belongs to the MoaA / NifB / PqqE family. P9WJS1 No intermediary metabolism and respiration P65384 P9WJS1 GO:0005525,GO:0006777,GO:0003824,GO:0046872,GO:0019008,GO:0051539 P9WJS1 NC_000962.3 Mycobrowser_v5 CDS 976872 978203 . - 0 Rv0878c PPE13 Function unknown PPE family protein PPE13 Rv0878c, (MTCY31.06c), len: 443 aa. PPE13, Member of the Mycobacterium tuberculosis PPE family, highly similar to many e.g. P4261|YHS6_MYCTU (517 aa), FASTA scores: opt: 1044, E(): 0, (47.4% identity in 397 aa overlap); MTV014_3, MTCI65_2, MTCY98_24, MTCY3C7_23, MTCY48_17, MTV004_5, MTV004_3, etc. Predicted to be an outer membrane protein (See Song et al., 2008). P9WI35 No PE/PPE Q10540 P9WI35 GO:0005886,GO:0016021 P9WI35 NC_000962.3 Mycobrowser_v5 CDS 979362 980228 . + 0 Rv0881 Rv0881 Causes methylation Possible rRNA methyltransferase (rRNA methylase) Rv0881, (MTCY31.09), len: 288 aa. Possible rRNA methyltransferase, highly similar to others and hypothetical proteins e.g. CAB76071.1|AL157953 putative rRNA methylase from Streptomyces coelicolor (272 aa); NP_421117.1|NC_002696 spoU rRNA methylase family protein from Caulobacter crescentus (268 aa); D90913_93|P74261 rRNA methylase from Synechocystis sp. (274 aa), FASTA scores: E(): 1.1e-13, (26.3% identity in 278 aa overlap); P18644|TSNR_STRCN rRNA methyltransferase from Streptomyces cyaneus (Streptomyces curacoi) (269 aa), FASTA scores: E(): 3.7e-08, (23.9% identity in 268 aa overlap); etc. Equivalent to AAK45146.1 from Mycobacterium tuberculosis strain CDC1551 (242 aa) but longer 46 aa. P9WFY3 No intermediary metabolism and respiration Q10543 P9WFY3 GO:0008173,GO:0006396,GO:0003723 2.1.1.- P9WFY3 NC_000962.3 Mycobrowser_v5 CDS 981424 982554 . - 0 Rv0884c serC Catalyzes the reversible interconversion of phosphoserine and 2-oxoglutarate to 3-phosphonooxypyruvate and glutamate. Require both in the major phosphorylated pathway of serine biosynthesis and in pyridoxine biosynthesis [catalytic activity: O-phospho-L-serine + 2-oxoglutarate = 3-phosphonooxypyruvate + L-glutamate]. Possible phosphoserine aminotransferase SerC (PSAT) Rv0884c, (MTCY31.12c), len: 376 aa. Possible serC, phosphoserine aminotransferase, equivalent to MLCB57_17 putative phosphoserine aminotransferase from Mycobacterium leprae (376 aa), FASTA scores: E(): 0, (87.5 identity in 376 aa overlap). Also highly similar to CAC08322.1|AL392149 putative aminotransferase from Streptomyces coelicolor (363 aa); and similar to other phosphoserine aminotransferases e.g. NP_386837.1|NC_003047 putative phosphoserine aminotransferase protein from Sinorhizobium meliloti (392 aa); P52878|SERC_METBA phosphoserine aminotransferase from Methanosarcina barkeri (370 aa); P10658|SERC_RABIT|RABEPIP_1 phosphoserine aminotransferase from Rabbit (370 aa), FASTA scores: opt: 271, E(): 3.5e-11, (24.5% identity in 368 aa overlap); etc. Belongs to class-V of pyridoxal-phosphate-dependent aminotransferases. Cofactor: pyridoxal phosphate. P9WQ73 No intermediary metabolism and respiration 2FYF P63514 P9WQ73 GO:0004648,GO:0005737,GO:0030170,GO:0008615,GO:0006564 2.6.1.52 P9WQ73 NC_000962.3 Mycobrowser_v5 CDS 988740 989861 . - 0 Rv0889c citA Involved in tricarboxylic acid cycle (KREBS cycle) [catalytic activity: citrate + CoA = acetyl-CoA + H2O + oxaloacetate]. Probable citrate synthase II CitA Rv0889c, (MTCY31.17c), len: 373 aa. Probable citA (alternate gene name: gltA), citrate synthase 2, highly similar to others e.g. CAB95899.1|AL359988 putative citrate synthase from Streptomyces coelicolor (387 aa); P39119|CISY_BACSU citrate synthase II from Bacillus subtilis (366 aa), FASTA scores: opt: 586, E(): 5.8e-30, (33.8% identity in 367 aa overlap); etc. Also similar to Rv0896|MTCY31.24 from Mycobacterium tuberculosis (29.2% identity in 274 aa overlap) and Rv1131. Contains PS00480 Citrate synthase signature. Belongs to the citrate synthase family. P9WPD3 No intermediary metabolism and respiration P63777 P9WPD3 GO:0004108,GO:0006099,GO:0044262 2.3.3.1 P9WPD3 NC_000962.3 Mycobrowser_v5 CDS 1002812 1003792 . + 0 Rv0899 ompA The protein behaved as a porin of low specific activity. Structural protein that may protect the integrity of the bacterium. Outer membrane protein A OmpA Rv0899, (MTCY31.27), len: 326 aa. OmpA, outer membrane protein A (See Senaratne et al., 1998). C-terminal region similar to C-terminus of many members of the OmpA family of outer membrane proteins, e.g. NP_458280.1|NC_003198 putative outer membrane protein from Salmonella enterica subsp. enterica serovar Typhi (220); NP_418008.1|NC_000913 putative outer membrane protein from Escherichia coli strain K12 (219 aa), FASTA scores: opt: 296, E(): 2.2e-11, (45.3% identity in 117 aa overlap); NP_231844.1|NC_002505 outer membrane protein OmpA from Vibrio cholerae (321 aa); Q05146|OMPA_BORAV outer membrane protein A precursor from Bordetella avium (194 aa); etc. A signal peptide sequence probably exists at the N-terminus. N-terminal domain is necessary and sufficient for membrane translocation (See Alahari et al., 2007). Contains PS00044 Bacterial regulatory proteins, lysR family signature. Belongs to the OmpA family. Pore-forming activity is pH-dependent. P9WIU5 No cell wall and cell processes 2KGS,2KGW,2KSM,2L26,2LBT,2LCA P65593 P9WIU5 GO:0016021,GO:0005886,GO:0009279 P9WIU5 NC_000962.3 Mycobrowser_v5 CDS 1004501 1005841 . - 0 Rv0902c prrB Sensor part of the two component regulatory system PRRA/PRRB. Thought to be involved in the environmental adaptation, specifically in an early phase of the intracellular growth. Two component sensor histidine kinase PrrB Rv0902c, (MTCY31.30c), len: 446 aa. PrrB, two-component sensor histidine kinase (see citations below), transmembrane protein, equivalent to MLCB57_26|NP_302403.1|NC_002677 sensor histidine kinase from Mycobacterium leprae (446 aa); and similar at C-termini to NP_301251.1|NC_002677 putative two-component system sensor kinase from Mycobacterium leprae (519 aa). C-terminus also similar to the C-termini of many sensor-like histidine kinase proteins e.g. P08336|CPXA_ECOLI|ECFB|SSD|EUP|B3911|Z5456|ECS4837 sensor protein from Escherichia coli strain K12 (457 aa), FASTA scores: opt: 364, E(): 1.7e-15, (27.1% identity in 398 aa overlap); CAB89748.1|AL354616 putative two-component histidine kinase from Streptomyces coelicolor (483 aa); CAB82845.1|AJ277081 putative histidine kinase from Amycolatopsis mediterranei (472 aa); etc. Also similar in part to Mycobacterium tuberculosis proteins Rv3764c (475 aa); and Rv0982 (504 aa). Thought to be induced at phagocytosis (see Graham & Clark-Curtiss 1999). P9WGK7 No regulatory proteins 1YS3,1YSR P0A5Z8 P9WGK7 GO:0016021,GO:0018106,GO:0005886,GO:0007165,GO:0000155,GO:0000160,GO:0005524 2.7.13.3 P9WGK7 NC_000962.3 Mycobrowser_v5 CDS 1006693 1008180 . - 0 Rv0904c accD3 This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA [catalytic activity ATP + acetyl-CoA + HCO(3)(-) = ADP + phosphate + malonyl-CoA]. Putative acetyl-coenzyme A carboxylase carboxyl transferase (subunit beta) AccD3 (accase beta chain) Rv0904c, (MTCY31.32c, MT0927), len: 495 aa. Putative accD3, acetyl-CoA carboxylase carboxyl transferase, beta subunit (carboxyltransferase subunit of acetyl-CoA carboxylase), highly similar in part to AAA63045.1|U15184 zinc finger protein from Mycobacterium leprae (201 aa). Also highly similar to others e.g. CAC42827.1|Y17592 putative carboxyltransferase subunit of acetyl-CoA carboxylase from Corynebacterium glutamicum (491 aa); CAB86110.1|AL163003 putative acetyl CoA carboxylase (alpha and beta subunits) from Streptomyces coelicolor (458 aa); Q54776|ACCD_SYNP7 acetyl-coenzyme A carboxylase carboxyl transferase subunit beta from Synechococcus sp. (305 aa); P12217|ACCD_MARPO acetyl-coenzyme A carboxylase carboxyl transferase subunit beta from Marchantia polymorpha (316 aa), FASTA scores: opt: 519, E():1.6e-24, (40.2% identity in 219 aa overlap); etc. Also similar to Rv3280, Rv2502c, etc from Mycobacterium tuberculosis. Belongs to the ACCD/PCCB family. P9WQH9 No lipid metabolism P63405 P9WQH9 GO:0003989,GO:0009317,GO:0006633,GO:0005524 6.4.1.2 P9WQH9 NC_000962.3 Mycobrowser_v5 CDS 1011731 1014124 . + 0 Rv0908 ctpE Metal cation-transporting ATPase; possibly catalyzes the transport of an undetermined metal cation with the hydrolysis of ATP [catalytic activity: ATP + H(2)O + undetermined metal cation(in) = ADP + phosphate + undetermined metal cation(out)]. Probable metal cation transporter ATPase P-type CtpE Rv0908, (MTCY21C12.02), len: 797 aa. Probable ctpE, metal cation-transporting ATPase P-type, transmembrane protein, E1-E2 family, highly similar to many e.g. AB93406.1|AL357524 putative integral membrane ATPase from Streptomyces coelicolor (802 aa); NP_346063.1|NC_003028 cation-transporting ATPase (E1-E2 family) from Streptococcus pneumoniae (778 aa); P37278|ATCL_SYNP7|PACL cation-transporting atpase from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (926 aa), FASTA scores: opt: 257, E(): 4.8e-33, (27.7% identity in 905 aa overlap); etc. Contains E1-E2 ATPases phosphorylation site (PS00154). Belongs to the cation transport ATPases family (E1-E2 ATPases). P9WPT1 No cell wall and cell processes P0A504 P9WPT1 GO:0006754,GO:0015662,GO:0016021,GO:0046872,GO:0005886,GO:0005524 3.6.3.- P9WPT1 NC_000962.3 Mycobrowser_v5 CDS 1018727 1019965 . - 0 Rv0914c Rv0914c Thought to be involved in degradative pathways such as fatty acid BETA_OXIDATION. Possible lipid carrier protein or keto acyl-CoA thiolase Rv0914c, (MTCY21C12.08c), len: 412 aa. Possible lipid carrier protein or keto acyl-CoA thiolase, highly similar to NP_421905.1|NC_002696 thiolase family protein from Caulobacter crescentus (407 aa); and similar to others e.g. NP_107896.1|NC_002678 3-ketoacyl-CoA thiolase from Mesorhizobium loti (392 aa); NP_385796.1|NC_003047 putative 3-ketoacyl-CoA thiolase protein from Sinorhizobium meliloti (389 aa); NP_275932.1|NC_000916 lipid-transfer protein (sterol or nonspecific) from Methanothermobacter thermautotrophicus (383 aa); AB55378.1|AL117263 possible 3-ketoacyl-CoA thiolase from Leishmania major (441 aa), FASTA scores: opt: 547, E(): 3.1e-26, (31.0% identity in 435 aa overlap); etc. Also similar to Rv2790c, Rv1627c, Rv0244, etc from Mycobacterium tuberculosis. Could belong to the thiolase family. I6XWJ8 No lipid metabolism O05906 I6XWJ8 GO:0008152,GO:0003988 2.3.1.16 I6XWJ8 NC_000962.3 Mycobrowser_v5 CDS 1022087 1023868 . + 0 Rv0917 betP High-affinity uptake of glycine betaine. Supposedly responsible for the translocation of the substrate across the membrane. Possible glycine betaine transport integral membrane protein BetP Rv0917, (MTCY21C12.11), len: 593 aa. Possible betP, glycine betaine transporter, integral membrane protein, highly similar to many transporters, mainly glycine betaine transporters, e.g. P54582|BETP_CORGL glycine betaine transporter from Corynebacterium glutamicum (Brevibacterium flavum) (595 aa), FASTA scores: opt: 1367, E(): 0, (42.7% identity in 504 aa overlap); T35264 probable BccT family transporter from Streptomyces coelicolor (578 aa); NP_243511.1|NC_002570 glycine betaine transporter from Bacillus halodurans (504 aa); NP_439848.1|NC_000907 high-affinity choline transport protein (betT) from Haemophilus influenzae (669 aa); etc. Seems to belong to the BCCT (TC 2.33) family of transporters. P9WPR7 No cell wall and cell processes P63695 P9WPR7 GO:0005886,GO:0006810,GO:0005215,GO:0016021 P9WPR7 NC_000962.3 Mycobrowser_v5 CDS 1030578 1031864 . - 0 Rv0924c mntH H(+)-stimulated, highly selective, divalent cation uptake system. Responsible for the translocation of the divalent metal across the membrane. Divalent cation-transport integral membrane protein MntH (BRAMP) (MRAMP) Rv0924c, (MTCY21C12.18c), len: 428 aa. MntH (alternative gene name: Nramp, Mramp), H+-dependent divalent cation-transport integral membrane protein (see citations below), equivalent to O69443|MNTH_MYCBO probable manganese transport protein MNTH (BRAMP) from Mycobacterium bovis (415 aa); and NP_302396.1|NC_002677 probable manganese transport protein from Mycobacterium leprae (426 aa). Also similar (but longer 51 aa in N-terminus) to AAA63075.1|U15184 SMF2 protein from Mycobacterium leprae (377 aa), FASTA scores: opt: 1780, E(): 0, (74.5% identity in 376 aa overlap). Also similar to many orthologues of the eukaryotic Nramp (natural resistance-associated macrophage protein), also known as mntH, e.g. NP_456951.1|NC_003198 manganese transport protein MntH from Salmonella enterica subsp. enterica serovar Typhi (413 aa); etc. Belongs to the NRAMP family. P9WIZ5 No cell wall and cell processes O05916 P9WIZ5 GO:0030145,GO:0006828,GO:0005886,GO:0015293,GO:0016021 P9WIZ5 NC_000962.3 Mycobrowser_v5 CDS 1036028 1037002 . + 0 Rv0929 pstC2 Involved in active transport of inorganic phosphate across the membrane (import); responsible for the translocation of the substrate across the membrane. This is one of the proteins required for binding-protein-mediated phosphate transport. Phosphate-transport integral membrane ABC transporter PstC2 Rv0929, (MTCY21C12.23), len: 324 aa. PstC2, phosphate-transport integral membrane ABC transporter (see citations below), highly similar to others e.g. NP_302394.1|NC_002677 membrane-bound component of phosphate transport from Mycobacterium leprae (319 aa); CAB88474.1|AL353816 phosphate ABC transport system permease protein from Streptomyces coelicolor (336 aa); NP_290359.1| NC_002655 high-affinity phosphate-specific transport system (cytoplasmic membrane component) from Escherichia coli strain O157:H7 (319 aa); etc. Also similar to Rv935|MTCY08D9.04c|PSTC1 probable transmembrane ABC transporter component of phosphate uptake system from Mycobacterium tuberculosis (338 aa). Contains binding-protein-dependent transport systems inner membrane component signature (PS00402). P9WG05 No cell wall and cell processes P0A630 P9WG05 GO:0016021,GO:0006817,GO:0005886,GO:0005315 P9WG05 NC_000962.3 Mycobrowser_v5 CDS 1039936 1041048 . - 0 Rv0932c pstS2 Involved in active transport of inorganic phosphate across the membrane (import). This is one of the proteins required for binding-protein-mediated phosphate transport. Periplasmic phosphate-binding lipoprotein PstS2 (PBP-2) (PstS2) Rv0932c, (MTCY08D9.07), len: 370 aa. PstS2, phosphate-binding lipoprotein component of inorganic phosphate transport system (see citations below), highly similar to AAF74819.1|AF137360_1|AF137360 periplasmic phosphate permease from Mycobacterium avium (369 aa); Rv0928|MTCY21C12.22|pstS3 phosphate-binding periplasmic lipoprotein from Mycobacterium tuberculosis (370 aa), FASTA scores: opt: 1601, E(): 0, (64.5% identity in 372 aa overlap); and Rv0934|MTCY08D9.05c|pstS1 phosphate-binding periplasmic lipoprotein from Mycobacterium tuberculosis (374 aa) (Mycobacterium tuberculosis seems to have three PstS-like proteins, others being Rv0928 and Rv0934c). Also highly similar to MTCY08D9.05c|P15712|PAB_MYCTU protein antigen B precursor from Mycobacterium tuberculosis (374 aa), FASTA scores: opt: 460, E(): 2.7e-20, (31.2% identity in 375 aa overlap). Contains prokaryotic membrane lipoprotein lipid attachment site (PS00013) at N-terminus so the leader peptide of 22 aa is probably removed. Belongs to family of phosphate receptors for bacterial ABC-type lipoprotein transporters. Appears to be co-transcribed with Rv0931c|pknD|mbk. P9WGT9 No cell wall and cell processes O05870 P9WGT9 GO:0006817,GO:0005886,GO:0005315 P9WGT9 NC_000962.3 Mycobrowser_v5 CDS 1042115 1043239 . + 0 Rv0934 pstS1 Involved in active transport of inorganic phosphate across the membrane (import). This is one of the proteins required for binding-protein-mediated phosphate transport. Periplasmic phosphate-binding lipoprotein PstS1 (PBP-1) (PstS1) Rv0934, (MTCY08D9.05c), len: 374 aa. PstS1 (previously known as phoS1 or phoS), phosphate-binding lipoprotein component of inorganic phosphate transport system (see citations below), highly similar to Rv0932c|MTCY08D9.07|pstS2 phosphate-binding periplasmic lipoprotein from Mycobacterium tuberculosis (370 aa), FASTA scores: opt: 460, E(): 5.9e-19, (31.2% identity in 375 aa overlap); and Rv0928|MTCY21C12.22|pstS3 phosphate-binding periplasmic lipoprotein from Mycobacterium tuberculosis (374 aa), FASTA scores: opt: 435, E():1.1e-17, (30.0% identity in 380 aa overlap) (Mycobacterium tuberculosis seems to have three PstS-like proteins, others being Rv0932c and Rv0928c). Also highly similar to MTCY08D9.05c|P15712|PAB_MYCTU protein antigen B precursor from Mycobacterium tuberculosis (374 aa), FASTA scores: opt: 2459, E(): 0, (100% identity in 374 aa overlap). Contains a prokaryotic membrane lipoprotein lipid attachment site (PS00013) at the N-terminus so the 23 aa leader peptide sequence is probably removed. Belongs to family of phosphate receptors for bacterial ABC-type lipoprotein transporters. P9WGU1 No cell wall and cell processes 1PC3 P15712 P9WGU1 GO:0006817,GO:0005886,GO:0005315 P9WGU1 NC_000962.3 Mycobrowser_v5 CDS 1046136 1048415 . + 0 Rv0938 ligD Involved in DNA double-strand break repair, by nonhomologous end joining (NHEJ). Interacts with KU (Rv0937c) [catalytic activity:ATP + {deoxyribonucleotide}(N) + {deoxyribonucleotide}(M) = AMP + diphosphate + {deoxyribonucleotide}(N+M)]. ATP dependent DNA ligase LigD (ATP dependent polydeoxyribonucleotide synthase) (thermostable DNA ligase) (ATP dependent polynucleotide ligase) (sealase) (DNA repair enzyme) (DNA joinase) Rv0938, (MTCY08D9.01c, MTCY10D7.36c), len: 759 aa. ligD, ATP-dependent DNA ligase, with its C-terminus similar to N-terminal parts of many ATP-dependent DNA ligases e.g. NP_250828.1|NC_002516 probable ATP-dependent DNA ligase from Pseudomonas aeruginosa (840 aa); NP_105436.1|NC_002678 ATP-dependent DNA ligase from Mesorhizobium loti (829 aa); CAB92891.1|AL356932 probable ATP-dependent DNA ligase from Streptomyces coelicolor (326 aa); etc. The N-terminal half shows similarity with hypothetical proteins from Mycobacterium tuberculosis Rv0269c and Rv3730c; and the C-terminal half with the DNA ligases Rv3731 and Rv3062. P9WNV3 No information pathways 1VS0,2IRU,2IRX,2IRY,2R9L,3PKY P71571 P9WNV3 GO:0003910,GO:0003896,GO:0006310,GO:0006281,GO:0006269,GO:0005524 P9WNV3 NC_000962.3 Mycobrowser_v5 CDS 1054247 1055008 . + 0 Rv0945 Rv0945 Function unknown; possibly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv0945, (MTCY10D7.29c), len: 253 aa. Probable short-chain dehydrogenase/reductase, similar to various dehydrogenases/reductases e.g. NP_346338.1|NC_003028 oxidoreductase (short chain dehydrogenase/reductase family) from Streptococcus pneumoniae (253 aa); AAB70845.1|AF019986|PksB from Dictyostelium discoideum (260 aa); AAF86624.1|U87786 clavaldehyde dehydrogenase from Streptomyces clavuligerus (247 aa); P37440|UCPA_ECOLI oxidoreductase from Escherichia coli (285 aa), FASTA scores: opt: 275, E(): 1.1e-12, (33.8% identity in 201 aa overlap); etc. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. P9WGR7 No intermediary metabolism and respiration P71564 P9WGR7 GO:0055114,GO:0016491 1.-.-.- P9WGR7 NC_000962.3 Mycobrowser_v5 CDS 1055024 1056685 . - 0 Rv0946c pgi Involved in glycolysis and in gluconeogenesis [catalytic activity: D-glucose 6-phosphate = D-fructose 6-phosphate]. Probable glucose-6-phosphate isomerase Pgi (GPI) (phosphoglucose isomerase) (phosphohexose isomerase) (phi) Rv0946c, (MTCY10D7.28), len: 553 aa. Probable pgi, glucose-6-phosphate isomerase, equivalent to NP_301236.1|NC_002677 glucose-6-phosphate isomerase from Mycobacterium leprae (554 aa); and P96803|G6PI_MYCSM glucose-6-phosphate isomerase from Mycobacterium smegmatis (442 aa). Also highly similar to others e.g. T36015 glucose-6-phosphate isomerase from Streptomyces coelicolor (551 aa); P11537|G6PI_ECOLI|GPI glucose-6-phosphate isomerase from Escherichia coli strains K12 and O157:H7 (549 aa), FASTA scores: opt: 1779, E(): 0, (51.4% identity in 554 aa overlap); etc. Contains PS00765 Phosphoglucose isomerase signature 1, and PS00174 Phosphoglucose isomerase signature 2. Belongs to the GPI family. P9WN69 No intermediary metabolism and respiration 2WU8 P64192 P9WN69 GO:0006094,GO:0004347,GO:0006096,GO:0005737 5.3.1.9 P9WN69 NC_000962.3 Mycobrowser_v5 CDS 1058260 1060575 . + 0 Rv0949 uvrD1 Involved in nucleotide excision repair. Has a 3'-5' helicase activity in presence of ATP. Preferred substrate being one with both single and double stranded regions of DNA. Probable ATP-dependent DNA helicase II UvrD1 Rv0949, (MTCY10D7.25c), len: 771 aa. Probable uvrD1, ATP dependent DNA helicase II (see citation below), equivalent to P_301239.1|NC_002677 putative ATP-dependent DNA helicase from Mycobacterium leprae (778 aa). Also highly similar to others e.g. CAB92660.1|AL356832 from Streptomyces coelicolor (831 aa) (N-terminus longer); P56255|PCRA_BACST from Bacillus stearothermophilus (724 aa); Q10213|YAY5_SCHPO from Schizosaccharomyces pombe (Fission yeast) (887 aa), FASTA scores: opt: 927, E(): 0, (33.5% identity in 659 aa overlap); etc. Also similar to several other UvrD-like proteins in Mycobacterium tuberculosis e.g. Rv3201c, Rv3198c, Rv3202c. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the UVRD subfamily of helicases. Note that previously known as uvrD. P9WMQ1 No information pathways P0A5A3 P9WMQ1 GO:0004003,GO:0003677,GO:0006281,GO:0006268,GO:0005737,GO:0005524 3.6.4.12 P9WMQ1 NC_000962.3 Mycobrowser_v5 CDS 1061964 1063127 . + 0 Rv0951 sucC Involved in tricarboxylic acid cycle [catalytic activity: ATP + succinate + CoA = ADP + succinyl-CoA + phosphate]. Probable succinyl-CoA synthetase (beta chain) SucC (SCS-beta) Rv0951, (MTCY10D7.23c), len: 387 aa. Probable sucC, succinyl-CoA synthetase, beta chain, equivalent to AL035500|MLCL373_3|NP_301241.1|NC_002677 succinyl-CoA synthase [beta] chain from Mycobacterium leprae (393 aa), FASTA score: (86.7% identity in 391 aa overlap). Also highly similar to others e.g. AB92671.1|AL356832 succinyl-CoA synthetase beta chain from Streptomyces coelicolor (394 aa); P25126|SUCC_THEFL succinyl-CoA synthetase beta chain from Thermus aquaticus (378 aa); P07460|SUCC_ECOLI succinyl-CoA synthetase beta chain from Escherichia coli (388 aa), FASTA scores: opt: 933, E(): 0, (41.0% identity in 390 aa overlap); etc. P9WGC5 No intermediary metabolism and respiration P71559 P9WGC5 GO:0046872,GO:0004775,GO:0006099,GO:0005524 6.2.1.5 P9WGC5 NC_000962.3 Mycobrowser_v5 CDS 1067561 1068208 . + 0 Rv0956 purN Involved in de novo purine biosynthesis (at the third step) [catalytic activity: 10-formyltetrahydrofolate + 5'-phosphoribosylglycinamide = tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide]. Probable 5'-phosphoribosylglycinamide formyltransferase PurN (GART) (gar transformylase) (5'-phosphoribosylglycinamide transformylase) Rv0956, (MTCY10D7.18c), len: 215 aa. Probable purN, 5'-phosphoribosylglycinamide formyltransferase, equivalent to AAF05726.1|AF191543_1|AF191543|PurN phosphoribosylglycinamide formyltransferase from Mycobacterium avium subsp. paratuberculosis (209 aa); and AL035500|MLCL373_7 from Mycobacterium leprae (215 aa), FASTA score: (79.4% identity in 214 aa overlap). Also highly similar to others e.g. BAA89443.1|AB003159 from Corynebacterium ammoniagenes (199 aa); NP_241498.1|NC_002570 from Bacillus halodurans (188 aa); P08179|PUR3_ECOLI|B2500 from Escherichia coli strain K12 (212 aa), FASTA scores: opt: 380, E(): 2.4e-18, (36.6% identity in 183 aa overlap); C-terminus of P16340|PUR2_DROPS trifunctional purine biosynthetic protein adenosine-3 from Drosophila pseudoobscura (Fruit fly) (1364 aa); etc. P9WHM5 No intermediary metabolism and respiration 3DA8,3DCJ P71554 P9WHM5 GO:0004644,GO:0006189 2.1.2.2 P9WHM5 NC_000962.3 Mycobrowser_v5 CDS 1068205 1069776 . + 0 Rv0957 purH Involved in de novo purine biosynthesis (at the ninth and tenth steps) [catalytic activity 1: 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-4-imidazolecarboxamide = tetrahydrofolate + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide] [catalytic activity 2: imp + H2O = 5-formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide]. Probable bifunctional purine biosynthesis protein PurH: phosphoribosylaminoimidazolecarboxamide formyltransferase (AICAR transformylase) (5'-phosphoribosyl-5-aminoimidazole-4-carboxamide formyltransferase) + inosinemonophosphate cyclohydrolase (imp cyclohydrolase) (inosinicase) (imp synthetase) (ATIC) Rv0957, (MTCY10D7.17c), len: 523 aa. Probable purH, bifunctional purine biosynthesis protein including 5'-phosphoribosyl-5-aminoimidazole-4-carboxamide formyltransferase and inosine-monophosphate (imp) cyclohydrolase, equivalent to AL035500|MLCL373_8 putative phosphoribosylaminoimidazolecarboxamide formyltransferase from Mycobacterium leprae (527 aa), FASTA score: (88.1% identity in 520 aa overlap); and AF05727.1|AF191543_2|AF191543|PurH from Mycobacterium avium subsp. paratuberculosis (527 aa). Also highly similar to others e.g. CAB92677.1|AL356832 bifunctional purine biosynthesis protein from Streptomyces coelicolor (523 aa); NP_388534.1|NC_000964 phosphoribosylaminoimidazole carboxy formyl formyltransferase + inosine-monophosphate cyclohydrolase from Bacillus subtilis (512 aa); P15639|PUR9_ECOLI phosphoribosylaminoimidazolecarboxamide formyltransferase from Escherichia coli (529 aa), FASTA scores: opt: 1147, E(): 0, (44.8% identity in 533 aa overlap); etc. Belongs to the PurH family. P9WHM7 No intermediary metabolism and respiration 3ZZM,4A1O P67541 P9WHM7 GO:0003937,GO:0004643,GO:0006188 3.5.4.10,2.1.2.3 P9WHM7 NC_000962.3 Mycobrowser_v5 CDS 1606386 1607972 . + 0 Rv1430 PE16 Function unknown PE family protein PE16 Rv1430, (MTCY493.24c), len: 528 aa. PE16, Member of the Mycobacterium tuberculosis PE family of proteins (see citation below), e.g. Y0D4_MYCTU|Q50594 (55.9% identity in 127 aa overlap). The C-terminus shows similarity to Q49633|LEPB1170_F3_112 hypothetical Mycobacterium leprae protein (391 aa), FASTA scores: opt: 342, E(): 1.2e-13, (29.8% identity in 292 aa overlap). Possible TMhelix aa 500-522. L7N697 No PE/PPE Q7D8G5 L7N697 L7N697 NC_000962.3 Mycobrowser_v5 CDS 1078743 1081055 . + 0 Rv0969 ctpV Metal cation-transporting ATPase; possibly catalyzes the transport of an undetermined metal cation with the hydrolysis of ATP [catalytic activity: ATP + H(2)O + undetermined metal cation(in) = ADP + phosphate + undetermined metal cation(out)]. Probable metal cation transporter P-type ATPase CtpV Rv0969, (MTCY10D7.05c), len: 770 aa. Probable ctpV, metal cation transporter P-type ATPase (transmembrane protein) (see citation below), part of cso operon, highly similar (except in N-terminus) to others e.g. NP_391230.1|NC_000964 similar to heavy metal-transporting ATPase from Bacillus subtilis (803 aa); P37279|ATCS_SYNP7|PACS cation-transporting ATPase from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (747 aa), FASTA scores: opt: 1851, E(): 0, (52.1% identity in 664 aa overlap); etc. Equivalent to AAK45246.1 from Mycobacterium tuberculosis strain CDC1551 (792 aa) but shorter 22 aa. Contains PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases). P9WPS3 No cell wall and cell processes P77894 P9WPS3 GO:0006754,GO:0004008,GO:0016021,GO:0046872,GO:0030001,GO:0005886,GO:0005524 3.6.3.- P9WPS3 NC_000962.3 Mycobrowser_v5 CDS 1179396 1180577 . + 0 Rv1057 Rv1057 Function unknown Conserved hypothetical protein Rv1057, (MTV017.10), len: 393 aa. Conserved hypothetical protein, some similarity to X84710|MMSAG_1 surface antigen of Methanosarcina mazeii (491 aa), FASTA scores: opt: 363, E():6.2e-15, (31.3% identity in 294 aa overlap). Regulated by MprA (Rv0981) under physiological conditions and environmental stress (SDS and Triton X-100) (See He et al., 2006). O53405 No conserved hypotheticals O53405 O53405 NC_000962.3 Mycobrowser_v5 CDS 1783309 1783623 . - 0 Rv1579c Rv1579c Unknown Probable PhiRv1 phage protein Rv1579c, (MTCY336.25), len: 104 aa. Probable phiRv1 phage protein (see citation below). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06611 No insertion seqs and phages O06611 NC_000962.3 Mycobrowser_v5 CDS 1083747 1085750 . - 0 Rv0973c accA2 This protein carries two functions: biotin carboxyl carrier protein and biotin carboxyltransferase. Involved in the first step of long-chain fatty acid synthesis [catalytic activity: ATP + biotin-carboxyl-carrier protein + CO(2) = ADP + phosphate + carboxybiotin-carboxyl-carrier protein]. Probable acetyl-/propionyl-coenzyme A carboxylase alpha chain (alpha subunit) AccA2: biotin carboxylase + biotin carboxyl carrier protein (BCCP) Rv0973c, (MTV044.01c, MTCY10D7.01), len: 667 aa. Probable accA2 (alternate gene name: bccA), acetyl-/propionyl-coenzyme A carboxylase (alpha subunit) [includes: biotin carboxylase ; biotin carboxyl carrier protein (BCCP)], highly similar to others e.g. CAB95892.1|AL359988 putative acetyl/propionyl CoA carboxylase alpha subunit from Streptomyces coelicolor (614 aa); NP_250702.1|NC_002516 probable acyl-CoA carboxylase alpha chain from Pseudomonas aeruginosa (655 aa); NP_420971.1|NC_002696 acetyl/propionyl-CoA carboxylase alpha subunit from Caulobacter crescentus ( 654 aa); NP_251581.1|NC_002516 probable biotin carboxylase/biotin carboxyl carrier protein from Pseudomonas aeruginosa (661 aa); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. Rv2501c|P46401|MTCY07A7.07c|BCCA_MYCTU|ACCA1 probable acetyl-/propionyl-coenzyme A carboxylase alpha chain (alpha subunit) (654 aa), FASTA scores, opt: 250, E(): 4e-09, (28.6% identity in 182 aa overlap); and Rv3285|MTCY71.25|ACCA3 (600 aa); Z83018|MTCY349_20 (1127 aa), FASTA scores: opt: 838, E(): 0, (40.2% identity in 500 aa overlap). Contains PS00867 Carbamoyl-phosphate synthase subdomain signature 2 and PS00188 Biotin-requiring enzymes attachment site. P71538 No lipid metabolism P71538 P71538 GO:0009374,GO:0004075,GO:0008152,GO:0005524 6.3.4.14 P71538 NC_000962.3 Mycobrowser_v5 CDS 1087348 1088496 . - 0 Rv0975c fadE13 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE13 Rv0975c, (MTV044.03c), len: 382 aa. Probable fadE13, acyl-CoA dehydrogenase, highly similar to many e.g. T35427 probable acyl-CoA dehydrogenase from Streptomyces coelicolor (382 aa); M74096|HUMACADL_1 Human long chain acyl-CoA dehydrogenase from Homo sapiens (430 aa), FASTA scores: opt: 819, E(): 0, (37.0% identity in 376 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. fadE20|Z98209|MTCY154_4 (386 aa), FASTA scores: (40.3% identity in 375 aa overlap). Contains PS00073 Acyl-CoA dehydrogenases signature 2. Belongs to the acyl-CoA dehydrogenases family. O86319 No lipid metabolism O86319 O86319 GO:0003995,GO:0055114,GO:0050660 1.3.99.- O86319 NC_000962.3 Mycobrowser_v5 CDS 1090373 1093144 . + 0 Rv0977 PE_PGRS16 Function unknown PE-PGRS family protein PE_PGRS16 Rv0977, (MTV044.05), len: 923 aa. PE_PGRS16, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to other PGRS-type sequences e.g. AL0091|MTV004_1 from Mycobacterium tuberculosis (1125 aa), FASTA score: (45.4% identity in 959 aa overlap); Z80225|MTCY441_4 from Mycobacterium tuberculosis (778 aa), FASTA score: (51.5% identity in 750 aa overlap); etc. Q79FU3 No PE/PPE Q79FU3 Q79FU3 Q79FU3 NC_000962.3 Mycobrowser_v5 CDS 1096822 1097508 . + 0 Rv0981 mprA Regulator part of a two component regulatory system (MPRAB system) Mycobacterial persistence regulator MRPA (two component response transcriptional regulatory protein) Rv0981, (MTV044.09), len: 228 aa. MprA, mycobacterial persistence regulator, a two-component response regulator whose expression is required for entrance into and maintenance of persistent infection (see citation below), equivalent to NP_301250.1|NC_002677 putative two-component response regulator from Mycobacterium leprae (228 aa); and highly similar to others from Mycobacterium leprae. Also highly similar to others e.g. AAG36759.1|AF119221_1|AF119221 response regulator from Corynebacterium glutamicum (232 aa); CAB88489.1|AL353816 putative two-component system response regulator from Streptomyces coelicolor (248 aa); BJY09666_1 two-component response regulator (ragA, ragB and rpoH3) from B.japonicum (226 aa), FASTA score: (43.8% identity in 224 aa overlap); BSAJ2571_44 two-component response regulator from Bacillus subtilis (228 aa), FASTA score: (46.4% identity in 224 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. Rv1033c (257 aa); Rv0903c (236 aa), FASTA score: (50.7 identity in 225 aa overlap); etc. Contains PS00217 Sugar transport proteins signature 2. Start changed since first submission (-2 aa). MprAB is involved in the regulation of genes in response to environmental stress (See He et al., 2006). P9WGM9 No regulatory proteins O53894 P9WGM9 GO:0006950,GO:0005737,GO:0009405,GO:0006355,GO:0006350,GO:0000156,GO:0000160,GO:0003677 P9WGM9 NC_000962.3 Mycobrowser_v5 CDS 1097508 1099022 . + 0 Rv0982 mprB Sensor part of a two component regulatory system (MPRAB system) Two component sensor kinase MprB Rv0982, (MTV044.10), len: 504 aa. MprB, two component sensor kinase, probable transmembrane protein (see citation below), equivalent to AL035500|MLCL373_16|NP_301251.1|NC_002677 putative two-component system sensor kinase from Mycobacterium leprae (519 aa), FASTA score: (81.0% identity in 521 aa overlap). Also highly similar to others (especially in C-terminal part) e.g. AAG36760.1|AF119221_2|AF119221 sensor kinase from Corynebacterium glutamicum (455 aa); CAB89748.1|AL354616 putative two-component histidine kinase from Streptomyces coelicolor (481 aa); X58793|SLCUTRS_2 sensor kinase from S.lividans (414 aa), FASTA scores: opt: 451, E(): 4.2e-21, (36.0% identity in 303 aa overlap); P30847|BAES_ECOLI sensor protein from Escherichia coli (467 aa), FASTA scores: opt: 412, E(): 1.3e-18, (30.4% identity in 336 aa overlap); etc. Also similar in C-terminal region to C-terminus of Rv0902c|Z73101|MTCY31_33 from Mycobacterium tuberculosis (446 aa), FASTA scores: opt: 423, E(): 2.6e-19, (28.4 identity in 462 aa overlap). MprAB is involved in the regulation of genes in response to environmental stress (See He et al., 2006). P9WGL1 No regulatory proteins O53895 P9WGL1 GO:0016021,GO:0009405,GO:0018106,GO:0004721,GO:0005886,GO:0006950,GO:0007165,GO:0000155,GO:0000160,GO:0005524 3.1.3.-,2.7.13.3 P9WGL1 NC_000962.3 Mycobrowser_v5 CDS 1101025 1101480 . - 0 Rv0985c mscL Ion channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell. Possible large-conductance ion mechanosensitive channel MscL Rv0985c, (MTV044.13c), len: 151 aa. Possible mscL, large conductance mechanosensitive ion channel (integral membrane protein) (see citations below, equivalent to AL035500|MLCL373_19|NP_301254.1|NC_002677 putative mechanosensitive channel protein from Mycobacterium leprae (154 aa), FASTA score: (71.0% identity in 155 aa overlap). Also highly similar to others e.g. NP_268999.1|NC_002737 putative large conductance mechanosensitive channel from Streptococcus pyogenes (120 aa); CAB90974.1|AL355832 putative mechanosensitive channel from Streptomyces coelicolor (156 aa); Q9X722|MSCL_CLOHI large-conductance mechanosensitive channel from Clostridium histolyticum (133 aa); Z83337|BSZ83337_6 large conductance mechanosensitive channel from Bacillus subtilis (130 aa), FASTA scores: opt: 248, E(): 8.4e-10, (39.0% identity in 136 aa overlap); U08371|ECU08371_1 large conductance mechanosensitive channel from Escherichia coli strain K-12 (136 aa), FASTA score: (36.6% identity in 134 aa overlap); etc. Belongs to the MscL family. P9WJN5 No cell wall and cell processes 2OAR P0A5K8 P9WJN5 GO:0005216,GO:0006811,GO:0005886,GO:0016021 P9WJN5 NC_000962.3 Mycobrowser_v5 CDS 1101803 1102549 . + 0 Rv0986 Rv0986 Thought to be involved in active transport of adhesion component across the membrane: involved in attachment and virulence. Responsible for energy coupling to the transport system. Probable adhesion component transport ATP-binding protein ABC transporter Rv0986, (MTV044.14), len: 248 aa. Probable ATP-binding protein ABC transporter supposedly involved in transport of adhesion component (see citation below), highly similar to many ATP-binding proteins e.g. AE0010|AE001033_8 ABC transporter ATP-binding protein from Archaeoglobus fulgidus (228 aa), FASTA scores: opt: 669, E(): 0, (45.7% identity in 219 aa overlap); CAB81857.1|AL161691 putative ABC-transporter ATP-binding protein from Streptomyces coelicolor (246 aa); X84019|ZMDNAGRP_4 glutamate uptake regulatory protein (grp) from Z.mobilis (232 aa), FASTA score: (44.4% identity in 225 aa overlap); Z99111|BSUB0008_108 from Bacillus subtilis (230 aa), FASTA score: (38.7% identity in 222 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). Believed to have been acquired by horizontal gene transfer (See Rosas-Magallanes et el., 2006; Becq et al., 2007). P9WQK1 No cell wall and cell processes O53899 P9WQK1 GO:0016887,GO:0006810,GO:0005524 P9WQK1 NC_000962.3 Mycobrowser_v5 CDS 1106405 1107382 . - 0 Rv0989c grcC2 Possible supplier of polyprenyl diphosphate. Probable polyprenyl-diphosphate synthase GrcC2 (polyprenyl pyrophosphate synthetase) Rv0989c, (MTCI237.03c), len: 325 aa. Probable grcC2, polyprenyl diphosphate synthetase, highly similar to NP_302483.1|NC_002677 polyprenyl diphosphate synthase component from Mycobacterium leprae (330 aa). Also similar to others (generally hepta or hexaprenyl e.g. NP_471378.1|NC_003212 protein similar to heptaprenyl diphosphate synthase component II (menaquinone biosynthesis) from Listeria innocua (321 aa); NP_371994.1|NC_002758 heptaprenyl diphosphate syntase component II from Staphylococcus aureus subsp. aureus Mu50 (319 aa); P55785|HEP2_BACST heptaprenyl diphosphate synthase component from Bacillus subtilis (323 aa), FASTA scores: opt: 496, E(): 1.4e-24, (31.4% identity in 306 aa overlap); etc. Also highly similar to Mycobacterium tuberculosis proteins e.g. Rv0562|grcC1|NP_215076.1|MTCY25D10.41 probable polyprenyl-diphosphate synthase (335 aa); Rv3383, Rv3398c, Rv2173, etc. Seems to belong to the FPP/GGPP synthetases family. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O05572 No intermediary metabolism and respiration O05572 O05572 GO:0016740,GO:0008299 2.5.1.- O05572 NC_000962.3 Mycobrowser_v5 CDS 1109272 1110192 . + 0 Rv0993 galU May play a role in stationary phase survival [catalytic activity: UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose]. UTP--glucose-1-phosphate uridylyltransferase GalU (UDP-glucose pyrophosphorylase) (UDPGP) (alpha-D-glucosyl-1-phosphate uridylyltransferase) (uridine diphosphoglucose pyrophosphorylase) Rv0993, (MTCI237.07), len: 306 aa. GalU, UTP--glucose-1-phosphate uridylyltransferase, equivalent to AL035500|MLCL373_22 putative UTP-glucose-1-phosphate uridylyltransferase from Mycobacterium leprae (306 aa), FASTA score: (89.7% identity in 302 aa overlap). Also highly similar to others e.g. AB59678.1|AL132674 UTP-glucose-1-phosphate uridylyltransferase from Streptomyces coelicolor (303 aa); NP_244519.1|NC_002570 UTP-glucose-1-phosphate uridylyltransferase from Bacillus halodurans (297 aa); P25520|GALU_ECOLI|B1236|Z2012|ECS17 UTP--glucose-1-phosphate uridylyltransferase from Escherichia coli strains K12 and O157:H7 (301 aa), FASTA scores: opt: 624, E(): 2.4e-33, (38.8% identity in 299 aa overlap); etc. Belongs to the prokaryotic UDPGP family. O05576 No intermediary metabolism and respiration O05576 O05576 GO:0009058,GO:0003983 2.7.7.9 O05576 NC_000962.3 Mycobrowser_v5 CDS 1125444 1127003 . - 0 Rv1007c metS It is probably essential for cell survival, being required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [catalytic activity: ATP + L-methionine + tRNA(met) = AMP + diphosphate + L-methionyl-tRNA(met)] Methionyl-tRNA synthetase MetS (MetRS) (methionine--tRNA ligase) Rv1007c, (MTCI237.24), len: 519 aa. metS (MetG), methionyl-tRNA synthetase, similar to many e.g. SYM_BACSU|P37465 methionyl-tRNA synthetase from Bacillus subtilus (664 aa), FASTA scores: opt: 1506, E(): 0, (44.9% identity in 492 aa overlap); similar to other Mycobacterium tuberculosis tRNA synthases e.g. Rv2448c, Rv1536, Rv0041. Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Belongs to class-I aminoacyl-tRNA synthetase family. Strong, to cysteinyl-tRNA synthetase. P9WFU5 No information pathways O05593 P9WFU5 GO:0005737,GO:0004825,GO:0006431,GO:0005524 6.1.1.10 P9WFU5 NC_000962.3 Mycobrowser_v5 CDS 1114748 1115749 . + 0 Rv0998 Rv0998 Unknown Conserved hypothetical protein Rv0998, (MTCI237.12), len: 333 aa. Conserved hypothetical protein, with cyclic nucleotide-binding domain in N-terminal part and GNAT (Gcn5-related N-acetyltransferase) domain in C-terminal part. See Vetting et al. 2005. Possibly cyclic nucleotide-dependent protein kinase, highly similar to NP_301261.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (353 aa); and AL035500|MLCL373.38|T45457 hypothetical protein from Mycobacterium leprae (143 aa), FASTA score: (61.5% identity in 143 aa overlap). Also similar to many hypothetical proteins and cyclic-NMP-dependent protein kinases (generally at C-terminus) e.g. N-terminus of SC9B10.09|T35878 hypothetical protein from Streptomyces coelicolor (1039 aa); P05987|KAPR_DICDI camp-dependent protein kinase regulatory chain from Dictyostelium discoideum (327 aa), FASTA scores: opt: 177, E(): 0.00036, (32.0% identity in 122 aa overlap); NP_104403.1|NC_002678 hypothetical protein (contains similarity to cAMP-dependent protein kinase regulatory subunit) from Mesorhizobium loti (151 aa); etc. Contains PS00889 Cyclic nucleotide-binding domain signature 2. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O05581 No conserved hypotheticals O05581 O05581 GO:0008152,GO:0008080 O05581 NC_000962.3 Mycobrowser_v5 CDS 1115767 1116525 . + 0 Rv0999 Rv0999 Unknown Unknown protein Rv0999, (MTCI237.13), len: 252 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). O05582 No conserved hypotheticals O05582 O05582 NC_000962.3 Mycobrowser_v5 CDS 1131625 1133259 . + 0 Rv1013 pks16 Potentially involved in some intermediate steps for the synthesis of a polyketide molecule which may be involved in secondary metabolism. Putative polyketide synthase Pks16 Rv1013, (MTCI237.30-MTCY10G2.36c), len: 544 aa. Putative pks16, polyketide synthase, similar to many e.g. N-terminus of Q50857|U24657 saframycin MX1 synthetase B (1770 aa), FASTA scores: opt: 526, E(): 1.4e-25, (29.3% identity in 542 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family. O05598 No lipid metabolism O05598 O05598 GO:0008152,GO:0003824 O05598 NC_000962.3 Mycobrowser_v5 CDS 1134785 1135465 . - 0 Rv1016c lpqT Unknown Probable conserved lipoprotein LpqT Rv1016c, (MTCY10G2.33), len: 226 aa. Probable lpqT, conserved lipoprotein. Similar to several Mycobacterium tuberculosis hypothetical proteins e.g. Rv0040c|Y0H3_MYCTU|P71697 Proline rich 28 kDA antigen (310 aa), FASTA scores: opt: 329, E(): 2e-17, (32.3% identity in 229 aa overlap); Rv0583c. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WK59 No cell wall and cell processes P96384 P9WK59 GO:0005886 P9WK59 NC_000962.3 Mycobrowser_v5 CDS 1146561 1147028 . + 0 Rv1025 Rv1025 Function unknown Conserved protein Rv1025, (MTCY10G2.24c), len: 155 aa. Conserved protein, similar to hypothetical protein AE001768|AE001768_4 Thermotoga maritima (170 aa) FASTA scores: opt: 254, E(): 9.5e-10, (35.7% identity in 143 aa overlap). P96375 No conserved hypotheticals P96375 P96375 NC_000962.3 Mycobrowser_v5 CDS 1147019 1147978 . + 0 Rv1026 Rv1026 Unknown. Could be involved in an adaptive process that allows bacteria to respond to amino acid starvation. Conserved protein Rv1026, (MTCY10G2.23c), len: 319 aa. Conserved protein. Similar to GPPA_ECOLI|P25552 guanosine-5'-triphosphate,3'-diphosphate pyrophoshatase from Escherichia coli (494 aa), FASTA scores: opt: 281, E(): 3.2e-11, (30.6% identity in 291 aa overlap). Equivalent to AL023514|MLCB4.02 hypothetical protein from Mycobacterium leprae (317 aa) (77.9% identity in 321 aa overlap). P96374 No virulence, detoxification, adaptation P96374 P96374 GO:0016787 P96374 NC_000962.3 Mycobrowser_v5 CDS 1152012 1153727 . + 0 Rv1029 kdpA One of the components of the high-affinity ATP-driven potassium transport (or KDP) system, which catalyzes the hydrolysis of ATP coupled with the exchange of hydrogen and potassium ions [catalytic activity: ATP + H(2)O + K(+)(out) = ADP + phosphate + K(+)(in)]. Probable potassium-transporting ATPase a chain KdpA (potassium-translocating ATPase a chain) (ATP phosphohydrolase [potassium-transporting] a chain) (potassium binding and translocating subunit A) Rv1029, (MTCY10G2.20c), len: 571 aa. Probable kdpA, potassium-transporting ATPase a chain (transmembrane protein), similar to others e.g. ATKA_ECOLI|P03959|KDPA|B0698 potassium-transporting ATPase A chain from Escherichia coli strain K12 (557 aa), FASTA scores: opt: 1763, E(): 0, (50.4% identity in 569 aa overlap); etc. Belongs to the KdpA family. P9WKF3 No cell wall and cell processes P65209 P9WKF3 GO:0016021,GO:0005886,GO:0006813,GO:0008556,GO:0005524 3.6.3.12 P9WKF3 NC_000962.3 Mycobrowser_v5 CDS 1153724 1155853 . + 0 Rv1030 kdpB One of the components of the high-affinity ATP-driven potassium transport (or KDP) system, which catalyzes the hydrolysis of ATP coupled with the exchange of hydrogen and potassium ions [catalytic activity:ATP + H(2)O + K(+)(out) = ADP + phosphate + K(+)(in)]. Probable potassium-transporting P-type ATPase B chain KdpB (potassium-translocating ATPase B chain) (ATP phosphohydrolase [potassium-transporting] B chain) (potassium binding and translocating subunit B) Rv1030, (MTCY10G2.19c), len: 709 aa. Probable kdpB, potassium-transporting P-type ATPase B chain (transmembrane protein), similar to others e.g. ATKB_ECOLI|P03960 potassium-transporting ATPase B chain from Escherichia coli strain K12 (682 aa), FASTA scores: opt: 1481, E(): 0, (63.4% identity in 686 aa overlap); etc. Very similar to AL078610|SCH35.47 H+/K+-exchanging ATPase chain B from Streptomyces coelicolor (707 aa), FASTA scores: opt: 2731, E(): 0, (71.6% identity in 676 aa overlap). Contains PS00154 E1-E2 ATPases phosphorylation site. P9WPU3 No cell wall and cell processes P63681 P9WPU3 GO:0006754,GO:0016021,GO:0000287,GO:0005886,GO:0006813,GO:0008556,GO:0005524 3.6.3.12 P9WPU3 NC_000962.3 Mycobrowser_v5 CDS 1157963 1158736 . - 0 Rv1033c trcR Sensor part of the two component regulatory system TRCS/TRCR. Involved in transcriptional autoactivation: TRCR activates its own expression by interacting with the at-rich sequence of the TRCR promoter. Two component transcriptional regulator TrcR Rv1033c, (MTCY10G2.16), len: 257 aa. TrcR, two-component regulatory protein (see citations below), similar to Q50825 two component response regulator from Mycobacterium tuberculosis (234 aa), FASTA scores: opt: 628, E(): 0, (46.0% identity in 226 aa overlap). Note that in vitro autophosphorylation of TrcS requires the presence of Mn2+or Ca2+as a divalent cation cofactor and subsequent transphosphorylation of TrcR is evident in the presence of TrcS-phosphate and Ca2+. L7N689 No regulatory proteins Q50806 L7N689 GO:0006355,GO:0000156,GO:0000160,GO:0003677 L7N689 NC_000962.3 Mycobrowser_v5 CDS 1176011 1176286 . - 0 Rv1053c Rv1053c Unknown Hypothetical protein Rv1053c, (MTV017.06c), len: 91 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53401 No conserved hypotheticals O53401 O53401 NC_000962.3 Mycobrowser_v5 CDS 1176928 1177242 . + 0 Rv1054 Rv1054 Use for sequence integration. Integrase is necessary for integration of a phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome. Probable integrase (fragment) Rv1054, (MTV017.07), len: 104 aa. Probable integrase (fragment), similar to Rv2309c|MTCY3G12_25|Z79702 hypothetical protein (shows similarity to integrases) from Mycobacterium tuberculosis (151 aa), FASTA scores: opt: 273, E(): 8.8e-13, (64.7% identity in 68 aa overlap); and to L39071|MSGINT_1 integrase from Mycobacterium paratuberculosis (191 aa), FASTA scores: opt: 105, E(): 0.9, (31.8% identity in 85 aaoverlap). This ORF continues in another frame as Rv1055|MTV017.08 but no error can be found to account for frameshift. Length extended since first submission (+36 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53402 No insertion seqs and phages O53402 NC_000962.3 Mycobrowser_v5 CDS 1783620 1783892 . - 0 Rv1580c Rv1580c Unknown Probable PhiRv1 phage protein Rv1580c, (MTCY336.24), len: 90 aa. Probable phiRv1 phage protein (see citation below). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06610 No insertion seqs and phages O06610 O06610 NC_000962.3 Mycobrowser_v5 CDS 1185741 1186823 . - 0 Rv1063c Rv1063c Function unknown Conserved hypothetical protein Rv1063c, (MTV017.16c), len: 360 aa. Conserved hypothetical protein, similar to P37053|YCHK_ECOLI hypothetical protein from Escherichia coli (314 aa), FASTA scores: opt: 487, E(): 7.2e-23, (32.7% identity in 321 aa overlap). Also partially similar to Rv3239c|MTCY20B11.14c. Belongs to the UPF0028 (SWS) family. P9WIY9 No conserved hypotheticals P67098 P9WIY9 GO:0004622,GO:0046470,GO:0005789 NC_000962.3 Mycobrowser_v5 CDS 1188421 1190424 . - 0 Rv1067c PE_PGRS19 Function unknown PE-PGRS family protein PE_PGRS19 Rv1067c, (MTV017.20c), len: 667 aa. PE_PGRS19, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002). Similar to Rv3388|MTV004.46 M. tuberculosis (731 aa), FASTA scores: opt: 2227, E(): 0, (55.6% identity in 710 aa overlap). Contains PS00583 pfkB family of carbohydrate kinases signature 1, probably fortuitous. Predicted to be an outer membrane protein (See Song et al., 2008). Q79FT3 No PE/PPE Q79FT3 Q79FT3 Q79FT3 NC_000962.3 Mycobrowser_v5 CDS 1195055 1196092 . - 0 Rv1071c echA9 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Possible enoyl-CoA hydratase EchA9 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv1071c, (MTV017.24c), len: 345 aa. Possible echA9, enoyl-CoA hydratase, equivalent to Y13803|B1306.06c putative enoyl-CoA hydratase/isomerase from Mycobacterium leprae (345 aa), FASTA scores: opt: 1799, E(): 0, (77.7% identity in 345 aa overlap). Also similar to many eukaryotic and prokaryotic enoyl-CoA hydratases e.g. NP_437984.1|NC_003078 putative enoyl-CoA hydratase protein from Sinorhizobium meliloti (356 aa); NP_420165.1|NC_002696 enoyl-CoA hydratase/isomerase family protein from Caulobacter crescentus (350 aa); Q19278 protein similar to enoyl-CoA hydratases from Caenorhabditis elegans (386), FASTA scores: opt: 787, E(): 0, (38.5% identity in 348 aa overlap); etc. O53419 No lipid metabolism O53419 O53419 GO:0016853,GO:0008152,GO:0004300 4.2.1.17 O53419 NC_000962.3 Mycobrowser_v5 CDS 1210595 1211383 . + 0 Rv1086 Rv1086 Catalyzes the first committed step in the synthesis of decaprenyl diphosphate, a molecule which has a central role in the biosynthesis of most features of the mycobacterial cell wall. Adds one isoprene unit to omega,E-geranyl diphosphate. The product, omega,E, Z-farnesyl diphosphate, is the putative substrate of Rv2361c product [catalytic activity: geranyl diphosphate + isopentenyl diphosphate = diphosphate + trans,trans-farnesyl diphosphate]. Short (C15) chain Z-isoprenyl diphosphate synthase (Z-FPP synthase) (Z-farnesyl diphosphate synthase) (Z-FPP synthetase) (Z-farnesyl diphosphate synthetase) (geranyltranstransferase) (farnesyl pyrophosphate synthetase) Rv1086, (MTV017.39), len: 262 aa. Short (C15) chain Z-isoprenyl diphosphate synthase (see citations below), equivalent to NP_302598.1|NC_002677 possible undecaprenyl pyrophosphate synthetase from Mycobacterium leprae (262 aa), similar to many hypothetical proteins and several potential members of the upp synthase family e.g. NP_296167.1|NC_001263 undecaprenyl diphosphate synthase from Deinococcus radiodurans (339 aa); P20182|YT14_STRFR Hypothetical protein from Streptomyces fradiae (259 aa), FASTA scores: opt: 840, E(): 0, (51.0% identity in 259 aa overlap); and P38118|YARF_CORGL Hypothetical protein from Corynebacterium glutamicicum (234 aa), FASTA scores: opt: 729, E(): 0, (56.0% identity in 209 aa overlap); etc. Also similar to Rv2361c|MTCY27.19 (296 aa) (35.6% identity in 233 aa overlap). Contains PS01066 Uncharacterized protein family UPF0015 signature. Seems to belong to the UPP synthetase family. P9WFF5 No cell wall and cell processes 2VFW,2VG0,2VG1 O53434 P9WFF5 GO:0007047,GO:0009252,GO:0008360,GO:0033850 2.5.1.68 P9WFF5 NC_000962.3 Mycobrowser_v5 CDS 1197231 1198082 . + 0 Rv1073 Rv1073 Function unknown Conserved hypothetical protein Rv1073, (MTV017.26), len: 283 aa. Conserved hypothetical protein, similar to several hypothetical mycobacterial proteins e.g. Rv1482c|Z79701|MTCY277.03 Mycobacterium tuberculosis (339 aa), FASTA scores: opt: 810, E(): 0, (47.4% identity in 272 aa overlap); Rv3555c|Z92774|MTCY6G11_2 Mycobacterium tuberculosis (289 aa), FASTA scores: opt: 704, E(): 0, (44.4% identity in 259 aa overlap); and Rv3517, etc., and GIR10|AF002133_10 Mycobacterium avium strain GIR10 (346 aa), FASTA scores: opt: 802, E(): 0, (48.1% identity in 270 aa overlap). O53421 No conserved hypotheticals O53421 O53421 NC_000962.3 Mycobrowser_v5 CDS 1198156 1199373 . - 0 Rv1074c fadA3 Function unknown, but supposedly involved in lipid degradation (beta oxidation). Probable beta-ketoacyl CoA thiolase FadA3 Rv1074c, (MTV017.27c), len: 405 aa. Probable fadA3, beta-ketoacyl CoA thiolase, highly similar to many involved in beta-oxidation e.g. CAB89028.1|AL353870 beta-ketoadipyl-CoA thiolase from Streptomyces coelicolor (395 aa); P77525|PAAJ_ECOLI probable beta-ketoadipyl CoA thiolase from Escherichia coli (401 aa), FASTA scores: opt: 1034, E(): 5.4e-56, (43.5% identity in 416 aa overlap) and X97452 acetyl-CoA acetyltransferase (thiolase) from Escherichia coli (401 aa), FASTA scores: opt: 1043, E(): 0, (43.4% identity in 415 aa overlap); Q43935|CATF_ACICA beta-ketoadipyl CoA thiolase from Acinetobacter calcoaceticus (401 aa), FASTA scores: opt: 992, E(): 0, (41.5% identity in 415 aa overlap); etc. Contains PS00737 Thiolases signature 2, and PS00445 FGGY family of carbohydrate kinases signature 2, although this is probably fortuitous. Belongs to the thiolase family. O53422 No lipid metabolism O53422 O53422 GO:0008152,GO:0008415 2.3.1.- O53422 NC_000962.3 Mycobrowser_v5 CDS 1201717 1203111 . + 0 Rv1077 cbs Thought to be involved in homocysteine transulfuration [catalytic activity: L-serine + L-homocysteine = cystathionine + H2O] Probable cystathionine beta-synthase Cbs (serine sulfhydrase) (beta-thionase) (hemoprotein H-450) Rv1077, (MTV017.30), len: 464 aa. Probable cbs (previously cysM2), cystathionine beta-synthase, similar throughout its length to many eukaryotic cystathionine beta-synthases e.g. P32232|CBS_RAT cystathionine beta-synthase (560 aa), FASTA scores: opt: 951, E(): 0, (40.2% identity in 450 aa overlap); also similar in N-terminal domain (aa 1 - 330) to Rv2334|MTCY98.03 CysK Mycobacterium tuberculosis (310 aa), FASTA scores: opt: 855, E(): 0, (46.8% identity in 314 overlap); and other cysteine synthase proteins e.g. Rv1336, Rv0848, etc. Contains PS00217 Sugar transport proteins signature 2 probably spurious. Belongs to the cysteine synthase/cystathionine beta-synthase family. P9WP51 No intermediary metabolism and respiration Q7D8W0 P9WP51 GO:0019343,GO:0005737,GO:0030170,GO:0004122 4.2.1.22 P9WP51 NC_000962.3 Mycobrowser_v5 CDS 1206520 1207386 . + 0 Rv1082 mca Mycothiol-dependent detoxification enzyme, involved in mycothiol biosynthesis. Mycothiol conjugate amidase Mca (mycothiol S-conjugate amidase) Rv1082, (MTV017.35), len: 288 aa. Mca, mycothiol conjugate amidase (see citation below), equivalent to NP_302547.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (290 aa), FASTA scores: opt: 1737, E(): 0, (86.4% identity in 287 aa overlap); and similar to Q54358|X79146 lmbE protein from Streptomyces lincolnensis (270 aa). Also similar to Rv1170|MTV005.06|MSHB GlcNAc-Ins deacetylase from Mycobacterium tuberculosis (303 aa), FASTA scores: opt: 411, E(): 9.4e-20, (35.8% identity in 299 aa overlap). P9WJN1 No virulence, detoxification, adaptation O53430 P9WJN1 P9WJN1 NC_000962.3 Mycobrowser_v5 CDS 1221959 1222786 . + 0 Rv1094 desA2 Thought to catalyze the principal conversion of saturated fatty acids to unsaturated fatty acids. Thought to convert stearoyl-ACP to oleoyl-ACP by introduction of a cis double bond between carbons delta-9 and delta-10 of the acyl chain [catalytic activity: stearoyl-[acyl-carrier protein] + AH2 + O2 = oleoyl-[acyl-carrier protein] + a + 2 H2O]. Possible acyl-[acyl-carrier protein] desaturase DesA2 (acyl-[ACP] desaturase) (stearoyl-ACP desaturase) Rv1094, (MTV017.47), len: 275 aa. Possible desA2, acyl-[acyl-carrier protein] desaturase (stearoyl-ACP desaturase), equivalent to AL049491|MLCB1222_15 from Mycobacterium leprae (275 aa), FASTA score: (78.1% identity in 274 aa overlap). Also weakly similar to plant stearoyl-acyl carrier protein desaturases, and very similar to U49839|MTV043.16C|Rv0824c enzyme desA1 from Mycobacterium tuberculosis (338 aa), FASTA scores: opt: 525, E(): 8.5e-30, (32.2% identity in 270 aa overlap); and to U15182|MLU15182_32 acyl-carrier protein desaturase precursor from Mycobacterium leprae (338 aa), FASTA scores: opt: 506, E(): 1.9e-28, (34.1% identity in 261 aa overlap). P9WNZ5 No lipid metabolism 1ZA0 O53442 P9WNZ5 GO:0006633,GO:0055114,GO:0046914,GO:0045300 1.14.19.- P9WNZ5 NC_000962.3 Mycobrowser_v5 CDS 1225263 1226144 . - 0 Rv1097c Rv1097c Unknown Probable membrane glycine and proline rich protein Rv1097c, (MTV017.50c), len: 293 aa. Probable membrane Gly-, Pro-rich protein, similar to Mycobacterium tuberculosis Rv2507|MTCY07A7. 13|Z95556 (273 aa), FASTA scores: opt: 219, E(): 0.023, (30.5% identity in 266 aa overlap); and Rv2507. Contains potential membrane spanning region (aa ~68-92). O53445 No cell wall and cell processes O53445 NC_000962.3 Mycobrowser_v5 CDS 1247127 1248077 . + 0 Rv1124 ephC Thought to be involved in detoxification reactions following oxidative damage to lipids [catalytic activity: an epoxide + H(2)O = a glycol]. Probable epoxide hydrolase EphC (epoxide hydratase) Rv1124, (MTCY22G8.13), len: 316 aa. Probable ephC, epoxide hydrolase (see citation below), similar to Q42566 epoxide hydrolase from Arabidopsis thaliana (321 aa), FASTA scores: opt: 298, E(): 8.2e-13, (27.6% identity in 333 aa overlap). Similar to other M. tuberculosis epoxide hydrolases and non-heme bromoperoxidases e.g. Rv1938, Rv3617, Rv3670, Rv3473c, etc. O06576 No virulence, detoxification, adaptation O06576 O06576 GO:0016787 3.3.2.3 O06576 NC_000962.3 Mycobrowser_v5 CDS 1226141 1227565 . - 0 Rv1098c fum Involved in the tricarboxylic acid cycle. Catalyzes the reversible hydration of fumarate to L-malate [catalytic activity: (S)-malate = fumarate + H2O] Probable fumarase Fum (fumarate hydratase) Rv1098c, (MTV017.51c), len: 474 aa. Probable fum, fumarase. Equivalent to AL049491|MLCB1222_11 Mycobacterium leprae (474 aa) (89.5 % identity in 467 aa overlap). Similar to many e.g. P14408|FUMH_RAT fumarate hydratase, mitochondrial precursor from Rattus norvegicus (507 aa), FASTA scores: opt: 1427, E(): 0, (52.3% identity in 461 aa overlap); and P05042|FUMC_ECOLI Fumarate hydratase class II from Escherichia coli (467 aa), FASTA scores: opt: 1355, E(): 0, (50.2% identity in 444 aa overlap). Contains PS00163 Fumarate lyases signature. P9WN93 No intermediary metabolism and respiration 3NO9 O53446 P9WN93 GO:0004333,GO:0006099,GO:0005737 4.2.1.2 P9WN93 NC_000962.3 Mycobrowser_v5 CDS 1232844 1233956 . - 0 Rv1106c Rv1106c Oxidizes 3-beta-hydroxysterols using NAD+ as cofactor 3-beta-hydroxysteroid dehydrogenase Rv1106c, (MTV017.59c), len: 370 aa. 3-beta-hydroxysteroid dehydrogenase (see Yang et al., 2007). Equivalent to AL049491|MLCB1222_7 Mycobacterium leprae (376 aa) (75.5% identity in 375 aa overlap). Highly similar to Q03704 NAD(P)-dependent cholesterol dehydrogenase from Nocardia sp. (364 aa), FASTA scores: opt: 1789, E(): 0, (74.5% identity in 361 aa overlap). Also similar to U32426|MCU32426_1 3-beta-hydroxy-Delta5-steroid dehydrogenase from Molluscum contagiosum virus (354 aa), FASTA scores: opt: 432, E(): 1.7e-22, (34.6% identity in 347 aa overlap). Also similar to series of Mycobacterium tuberculosis hypothetical proteins described as sugar epimerases or dehydratases e.g. Rv3634c, Rv3784, Rv3464, etc. The transcription of this CDS seems to be activated specifically in host granulomas (see Ramakrishnan et al., 2000). P9WQP7 No intermediary metabolism and respiration O53454 P9WQP7 GO:0016853,GO:0055114,GO:0006694,GO:0003854 1.1.1.- P9WQP7 NC_000962.3 Mycobrowser_v5 CDS 1357293 1357625 . - 0 Rv1214c PE14 Function unknown PE family protein PE14 Rv1214c, (MTCI364.26c), len: 110 aa. PE14, Member of Mycobacterium tuberculosis PE family (see citation below), appears to be frameshifted but sequence appears to be correct. The 5'-end is atypical as first 9 aa appear to be missing. L7N6A7 No PE/PPE Q7D8L5 L7N6A7 L7N6A7 NC_000962.3 Mycobrowser_v5 CDS 1236185 1237192 . + 0 Rv1110 lytB2 Unknown. In other organisms, LYTB product is involved in penicillin tolerance and control of the stringent response. Probable LYTB-related protein LytB2 Rv1110, (MTV017.63), len: 335 aa. Probable lytB2, LytB-related protein, equivalent to AL049491|MLCB1222_3 from Mycobacterium leprae (335 aa), FASTA score: (82.9% identity in 333 aa overlap). Also similar to LytB proteins from many bacteria (appears to have N-terminal extension) e.g. P22565|LYTB_ECOLI|B0029|Z0034|ECS0032 LYTB protein from Escherichia coli strains K12 and O157:H7 (316 aa), FASTA scores: opt: 1041, E():0, (52.4% identity in 309 aa overlap); etc. Also very similar to another LytB-related protein from Mycobacterium tuberculosis: LytB1|Rv3382c|MTV004.40c (329 aa), FASTA scores: opt: 975, E(): 0, (51.3% identity in 312 aa overlap). Belongs to the LytB family. P9WKG1 No cell wall and cell processes P0A5I0 P9WKG1 GO:0051745,GO:0019288,GO:0046872,GO:0055114,GO:0016114,GO:0051538 1.17.1.2 P9WKG1 NC_000962.3 Mycobrowser_v5 CDS 1249932 1251404 . - 0 Rv1127c ppdK Catalyzes the reversible phosphorylation of pyruvate and phosphate [catalytic activity: ATP + pyruvate + phosphate = AMP + phosphoenolpyruvate + diphosphate] Probable pyruvate, phosphate dikinase PpdK Rv1127c, (MTCY22G8.16c), len: 490 aa. Probable ppdK, Pyruvate, phosphate dikinase. Equivalent (but shorter) to Z94723|MLCB33_16 ppdK from Mycobacterium leprae (601 aa) (71.8% identity in 478 aa overlap). Highly similar to N-terminus of PODK_CLOSY|P22983 pyruvate, phosphate dikinase from Clostridium symbiosum (873 aa), FASTA scores: opt: 786, E(): 0, (37.4% identity in 514 aa overlap). O06579 No intermediary metabolism and respiration O06579 O06579 GO:0016301,GO:0016310,GO:0050242,GO:0005524 2.7.9.1 O06579 NC_000962.3 Mycobrowser_v5 CDS 1256132 1257313 . + 0 Rv1131 prpC Involved in methyl citrate cycle [catalytic activity: propionyl-CoA + oxaloacetate + H2O = 2-methylcitrate + CoA] Probable methylcitrate synthase PrpC Rv1131, (MTCY22G8.20), len: 393 aa. Probable prpC, methylcitrate synthase (MCS) (previously known as gltA1) , highly similar to CISY_MYCSM|P26491 citrate synthase from Mycobacterium smegmatis (375 aa), FASTA scores: opt:1942, E(): 0, (80.0% identity in 375 aa overlap). Also similar to two other M. tuberculosis citrate synthases, Rv0896c|MTCY31.24|gltA2 (431 aa), FASTA score: (33.1% identity in 381 aa overlap) and Rv0889|MTCY31.17c|citA (373 aa), FASTA score: (31.8% identity in 371 aa overlap). Contains PS00480 Citrate synthase signature. Belongs to the citrate synthase family. I6Y9Q3 No intermediary metabolism and respiration 3HWK O08395 I6Y9Q3 GO:0044262,GO:0004108,GO:0005737,GO:0006099,GO:0008415 2.3.3.1 I6Y9Q3 NC_000962.3 Mycobrowser_v5 CDS 1259067 1261346 . - 0 Rv1133c metE Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation (pathway: terminal step in the de novo biosynthesis of methionine) [catalytic activity: 5-methyltetrahydropteroyltri-L-glutamate + L- homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine.] Probable 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase MetE (methionine synthase, vitamin-B12 independent isozyme) Rv1133c, (MTC22G8.22), len: 759 aa (start site chosen by homology). Probable metE, 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase, highly similar to others e.g. METE_ECOLI|P25665 Escherichia coli (752 aa), FASTA scores: opt: 2251, E(): 0, (48.1% identity in 756 aa overlap). Equivalent to Z94723|MLCB33_14 metE from M. leprae (760 aa) (85.3% identity in 755 aa overlap). Belongs to the vitamin-B12 independent methionine synthase family. P9WK07 No intermediary metabolism and respiration P65340 P9WK07 GO:0009086,GO:0008270,GO:0003871 2.1.1.14 P9WK07 NC_000962.3 Mycobrowser_v5 CDS 1269152 1269958 . - 0 Rv1142c echA10 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Probable enoyl-CoA hydratase EchA10 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv1142c, (MTCI65.09c), len: 268 aa. Probable echA10, enoyl-CoA hydratase, similar to others e.g. CAA66096.1|X97452 enoyl-CoA isomerase from Escherichia coli (262 aa), FASTA scores: opt: 525, E(): 1.3e-26, (35.1% identity in 251 aa overlap); NP_420658.1|NC_002696 enoyl-CoA hydratase/isomerase family protein from Caulobacter crescentus (267 aa); NP_438092.1|NC_003078 putative enoyl-CoA hydratase protein from Sinorhizobium meliloti (263 aa); etc. Also similarity with naphthoate synthases. Also highly similar to upstream ORF Rv1141c|MTCI65.08c|echA11 probable enoyl-CoA hydratase from Mycobacterium tuberculosis (268 aa), FASTA score: opt: 1225, E(): 0, (72.3% identity in 267 aa overlap). O06542 No lipid metabolism O06542 O06542 GO:0016853,GO:0008152,GO:0004300 4.2.1.17 O06542 NC_000962.3 Mycobrowser_v5 CDS 1273355 1274767 . + 0 Rv1146 mmpL13b Unknown. Thought to be involved in fatty acid transport. Probable conserved transmembrane transport protein MmpL13b Rv1146, (MTCI65.13), len: 470 aa. Probable mmpL13b, conserved transmembrane transport protein (see citation below), member of RND superfamily, showing some similarity to putative Mycobacterial and Streptomyces membrane proteins e.g. Q53902|C40046 antibiotic transport-associated protein from Streptomyces coelicolor (711 aa), FASTA scores: opt: 193, E(): 2.1e-05, (28.9% identity in 394 aa overlap); etc. Could be in frame with previous ORF mmpL13A|Rv1145, but no sequence error apparent to account for this; sequence is identical in M. tuberculosis strain CDC1551, and Mycobacterium bovis strain AF2122/97. Belongs to the MmpL family. O06546 No cell wall and cell processes O06546 O06546 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1937313 1938149 . + 0 Rv1709 scpA Involved in chromosome structure and partitioning Possible segregation and condensation protein ScpA Rv1709, (MTCI125.31), len: 278 aa. Possible scpA, segregation and condensation protein, similar to e.g. P35154|YPUG_BACSU from Bacillus subtilis (251 aa), FASTA scores: opt: 271, E(): 8.2e-10, (27.0% identity in 248 aa overlap); Q9S230|SCI51.10C|AL109848 from Streptomyces coelicolor (264 aa), FASTA scores: opt: 855, E(): 0, (56.8% identity in 257 aa overlap). Equivalent to Q49888|MLC1351.05C|Z95117 from Mycobacterium leprae (268 aa), FASTA scores: (78.9% identity in 251 aa overlap). O33208 No cell wall and cell processes O33208 O33208 GO:0051301,GO:0007059,GO:0005737,GO:0007049 O33208 NC_000962.3 Mycobrowser_v5 CDS 1284179 1284862 . - 0 Rv1158c Rv1158c Function unknown Conserved hypothetical ala-, pro-rich protein Rv1158c, (MTCI65.25c), len: 227 aa. Conserved hypothetical Ala-, Pro-rich protein, similar to other proline rich proteins and extensins e.g. MMSAP62|g633250 house mouse (485 aa), FASTA scores: opt: 367, E(): 1.2e-08, (36.3% identity in 212 aa overlap). Has hydrophobic stretch at N-terminus suggestive of secretion signal. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06556 No conserved hypotheticals O06556 O06556 NC_000962.3 Mycobrowser_v5 CDS 1284992 1286287 . + 0 Rv1159 pimE Polyprenol-phosphate-mannose dependent mannosyltransferase involved in phosphatidylinositol mannoside synthesis Mannosyltransferase PimE Rv1159, (MTCI65.26), len: 431 aa. PimE, mannosyltransferase (see Morita et al., 2006) Conserved transmembrane protein, similar to others in Mycobacterium tuberculosis e.g. Rv2181|MTCY21D4.13 (560 aa), FASTA scores: opt: 172; E(): 0.00035, (25.0% identity in 332 aa overlap). Belongs to the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). P9WN01 No cell wall and cell processes O06557 P9WN01 GO:0009405,GO:0008654,GO:0005886,GO:0016740,GO:0016021 2.4.1.- P9WN01 NC_000962.3 Mycobrowser_v5 CDS 1291065 1292741 . + 0 Rv1162 narH Nitrate reduction [catalytic activity: nitrite + acceptor = nitrate + reduced acceptor]. Probable respiratory nitrate reductase (beta chain) NarH Rv1162, (MTCI65.29), len: 558 aa. Probable narH, respiratory nitrate reductase beta chain. Similar to others e.g. NARH_BACSU|P42176 nitrate reductase beta chain from Bacillus subtilis (487 aa), FASTA scores: opt: 2049, E(): 0, (56.8% identity in 488 aa overlap); etc. Contains PS00190 cytochrome c family heme-binding site signature. O06560 No intermediary metabolism and respiration O06560 GO:0008940,GO:0009325,GO:0055114,GO:0042126 1.7.99.4 O06560 NC_000962.3 Mycobrowser_v5 CDS 1293406 1294146 . + 0 Rv1164 narI Nitrate reduction [catalytic activity: nitrite + acceptor = nitrate + reduced acceptor]. Probable respiratory nitrate reductase (gamma chain) NarI Rv1164, (MTCI65.31), len: 246 aa. Probable narI, respiratory nitrate reductase gamma chain. Similar to others e.g. NARI_BACSU|P42177 nitrate reductase gamma chain from Bacillus subtilis (223 aa), FASTA scores: opt: 652, E(): 0; (41.6% identity in 221 aa overlap); etc. Highly similar to C-terminal part of Rv1736c|MTCY04C12.21c|NARX probable nitrate reductase (gamma chain) from Mycobacterium tuberculosis (68.6% identity in 239 aa overlap). O06562 No intermediary metabolism and respiration O06562 O06562 GO:0009325,GO:0055114,GO:0008940 1.7.99.4 O06562 NC_000962.3 Mycobrowser_v5 CDS 1370920 1371477 . + 0 Rv1228 lpqX Unknown Probable lipoprotein LpqX Rv1228, (MTCI61.11), len: 185 aa. Probable lipoprotein LpqX. Contains possible signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O33224 No cell wall and cell processes O33224 O33224 NC_000962.3 Mycobrowser_v5 CDS 1294168 1296054 . + 0 Rv1165 typA Unknown; probably interacts with the ribosomes in a GTP dependent manner Possible GTP-binding translation elongation factor TypA (tyrosine phosphorylated protein A) (GTP-binding protein) Rv1165, (MTV005.01-MTCI65.32), len: 628 aa. Possible typA (alternate gene name: bipA), GTP-binding translation elongation factor, similar to several e.g. P32132|TYPA_ECOLI|BIPA|B387 Escherichia coli (591 aa); YIHK_SYNY3|P72749 gtp-binding protein TYPA/BIPA homolog from synechocystis sp. (597 aa), FASTA scores: E(): 0, (46.9% identity in 610 aa overlap); and to elongation factor EF-G from many organims e.g. EFG_MICLU|P09952 micrococcus luteus (701 aa), FASTA scores: E(): 3e-24, (29.8% identity in 500 aa overlap). Belongs to the GTP-binding elongation factor family, TYPA subfamily. O06563 No information pathways O06563 O06563 GO:0003924,GO:0005622,GO:0003746,GO:0005525 O06563 NC_000962.3 Mycobrowser_v5 CDS 1300304 1301215 . + 0 Rv1170 mshB Involved in mycothiol biosynthesis. 1-D-myo-inosityl-2-acetamido-2-deoxy-alpha-D-glucopyranoside (GlcNAc-Ins) is converted to 1-D-myo-inosityl-2-amino-2-deoxy-alpha-D-glucopyranoside (GlcN-Ins) by this enzyme. Seems to possess weak mycothiol conjugate amidase activity but SHOWS substantial deacetylation activity with 1-D-myo-inosityl-2-acetamido-2-deoxy-alpha-D-glucopyranoside (GlcNAc-Ins), a hypothetical mycothiol biosynthetic precursor. GlcNAc-Ins is an intermediate in MSH biosynthesis. N-acetyl-1-D-myo-inosityl-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase MshB (GlcNAc-Ins deacetylase) Rv1170, (MTV005.06), len: 303 aa. MshB, N-Acetyl-1-D-myo-Inosityl-2-Amino-2-Deoxy-alpha-D-Glucopyranoside Deacetylase (GlcNAc-Ins deacetylase) (see citation below), similar to Q54358|X79146 lmbE gene from Streptomyces lincolnensis (270 aa), FASTA scores: opt: 308, E(): 1.2e-15, (32.0% identity in 278 aa overlap). Also similar to Rv1082|MCA Mycothiol conjugate amidase from Mycobacterium tuberculosis (288 aa). P9WJN3 No intermediary metabolism and respiration 1Q74,1Q7T O50426 P9WJN3 P9WJN3 NC_000962.3 Mycobrowser_v5 CDS 1302931 1305501 . + 0 Rv1173 fbiC Essential for coenzyme F420 production: participates in a portion of the F420 biosynthetic pathway between pyrimidinedione and FO (biosynthesis intermediate), before the deazaflavin ring is formed. Probable F420 biosynthesis protein FbiC Rv1173, (MTV005.09), len: 856 aa. Probable fbiC, F420 biosynthesis protein, equivalent to AAL91922|FBIC F420 biosynthesis protein fbiC from Mycobacterium bovis BCG (856 aa) (see citation below). The N-terminus (aa 80-420) is similar to Y446_METJA|Q57888 hypothetical protein mj0446 from methanococcus jannaschii (361 aa), FASTA scores: opt: 801, E(): 0, (41.2% identity in 337 aa overlap); and the C-terminus region (aa 530-856) is similar to e.g. YE31_METJA|Q58826 hypothetical protein mj1431 from methanococcus jannaschii (359 aa), FASTA scores: opt: 1089, E(): 0, (48.7% identity in 337 aa overlap). P9WP77 No intermediary metabolism and respiration O50429 P9WP77 GO:0009108,GO:0046872,GO:0016765,GO:0051539 2.5.1.- P9WP77 NC_000962.3 Mycobrowser_v5 CDS 1394179 1395918 . + 0 Rv1250 Rv1250 Thought to be involved in transport of drug across the membrane (export): drug resistance by an export mechanism (conferes resistance to toxic compounds by removing them for the cells). Responsible for the translocation of the substrate across the membrane. Probable drug-transport integral membrane protein Rv1250, (MTV006.22), len: 579 aa. Probable drug-transport integral membrane protein, member of major facilitator superfamily (MFS), highly similar to several including P39886|TCMA_STRGA tetracenomycin C resistance protein from Streptomyces glaucescens (538 aa), FASTA scores: opt: 847, E(): 0, (32.9% identity in 517 aa overlap); etc. Also similar to MTCY20B11.14c|Rv3239C from Mycobacterium tuberculosis (1048 aa), FASTA scores: opt: 629, E(): 6.7e-13, (31.9% identity in 423 aa overlap). P9WG87 No cell wall and cell processes O50465 P9WG87 GO:0005886,GO:0055085,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1309364 1310452 . + 0 Rv1178 Rv1178 Function unknown; probably involved in cellular metabolism. Probable aminotransferase Rv1178, (MTV005.14), len: 362 aa. Probable aminotransferase, weak similarity to many aspartate aminotransferases e.g. Q55679|D64000 SLL0006 aspartate aminotransferase from Synechocystis sp. (394 aa), FASTA scores: opt: 218, E(): 1.3e-25, (32.5% identity in 379 aa overlap). Contains PS00105 Aminotransferases class-I pyridoxal-phosphate attachment site. Also similar to Mycobacterium tuberculosis aminotransferases Rv2294, Rv0075, etc. O50434 No intermediary metabolism and respiration O50434 O50434 GO:0009058,GO:0030170,GO:0008483,GO:0016847 2.6.1.- O50434 NC_000962.3 Mycobrowser_v5 CDS 1310480 1313299 . - 0 Rv1179c Rv1179c Unknown Unknown protein Rv1179c, MTV005.15c, len: 939 aa. Unknown protein. O50435 No conserved hypotheticals O50435 O50435 GO:0003677,GO:0016787,GO:0005524 NC_000962.3 Mycobrowser_v5 CDS 1321520 1324528 . + 0 Rv1183 mmpL10 Unknown. Thought to be involved in fatty acid transport. Probable conserved transmembrane transport protein MmpL10 Rv1183, (MTV005.19), len: 1002 aa. Probable mmpL10, conserved transmembrane transport protein (see Tekaia et al., 1999), member of RND superfamily, similar to many Mycobacterial hypothetical membrane proteins e.g. Q49619|U00010 from Mycobacterium leprae (1008 aa), FASTA scores: opt: 4545, E(): 0, (70.6% identity in 978 aa overlap); etc. Belongs to the MmpL family. P9WJU1 No cell wall and cell processes P65372 P9WJU1 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1329390 1331021 . + 0 Rv1187 rocA Involved in the arginase pathway [catalytic activity: 1-pyrroline-5-carboxylate + NAD(+) + H(2)O = L-glutamate + NADH] Probable pyrroline-5-carboxylate dehydrogenase RocA Rv1187, (MTV005.23), len: 543 aa. Probable rocA, pyrroline-5-carboxylate dehydrogenase, similar to many e.g. PUT2_HUMAN|P30038 human delta-1-pyrroline-5-carboxylate dehydrogenase (563 aa), FASTA scores: opt: 1596, E():0, (46.0% identity in 531 aa overlap). Also similar to other Mycobacterium tuberculosis hypothetical dehydrogenases e.g. Rv0768, Rv2858c, etc. Contains PS00687 Aldehyde dehydrogenases glutamic acid active site and PS00070 Aldehyde dehydrogenases cysteine active site. O50443 No intermediary metabolism and respiration O50443 O50443 GO:0005759,GO:0055114,GO:0006561,GO:0003842 1.5.1.12 O50443 NC_000962.3 Mycobrowser_v5 CDS 1335794 1337215 . + 0 Rv1193 fadD36 Function unknown, but supposedly involved in lipid degradation. Probable fatty-acid-CoA ligase FadD36 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv1193, (MTCI364.05), len: 473 aa. Probable fadD36, fatty-acid-CoA synthetase, highly similar to Q50017|U15181 4-coumarate-CoA ligase from Mycobacterium leprae (476 aa), FASTA scores: opt: 2594, E(): 0, (81.3% identity in 476 aa overlap). Also highly similar to others e.g. CAB86109.1|AL163003 putative fatty acid synthase from Streptomyces coelicolor (485 aa); LCFA_ECOLI|P29212 long-chain-fatty-acid--CoA ligase from Escherichia coli (561 aa), FASTA scores: opt: 605, E(): 8.4e-30, (33.0% identity in 364 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family. O05295 No lipid metabolism O05295 O05295 GO:0008152,GO:0016874 6.2.1.- O05295 NC_000962.3 Mycobrowser_v5 CDS 1341358 1342605 . - 0 Rv1199c Rv1199c Possibly required for the transposition of the insertion element IS1081. Possible transposase Rv1199c, (MTCI364.11c), len: 415 aa. Possible transposase for IS1081, identical to TRA1_MYCBO|P35882 transposase for insertion sequence element (415 aa); region identical to MTCY441.35 (100.0% identity in 261 aa overlap); and almost identical to MTCY10G2.02c (415 aa) (99.8% identity in 415 aa overlap). Contains PS01007 Transposases, Mutator family, signature, PS00435 Peroxidases proximal heme-ligand signature. P60230 No insertion seqs and phages P60230 P60230 GO:0004803,GO:0006313,GO:0003677 P60230 NC_000962.3 Mycobrowser_v5 CDS 1345260 1346324 . + 0 Rv1202 dapE Involved in biosynthesis of diaminopimelate and lysine from aspartate semialdehyde (at the fifth step) [catalytic activity: N-succinyl-ll-2,6-diaminoheptanedioate + H2O = succinate + ll-2,6-diaminoheptanedioate] Probable succinyl-diaminopimelate desuccinylase DapE Rv1202, (MTCI364.14), len: 354 aa. Probable dapE, succinyl-diaminopimelate desuccinylase, similar to DAPE_CORGL|Q59284 succinyl-diaminopimelate desuccinylase from Corynebacterium glutamicum (369 aa), FASTA scores: opt: 1301, E(): 0, (55.7% identity in 359 aa overlap), highly similar to Q49949|U1756G (400 aa), FASTA scores: opt: 2045, E(): 0, (87.0% identity in 354 aa overlap). P9WHS9 No intermediary metabolism and respiration Q7D8M5 P9WHS9 GO:0005515,GO:0006508,GO:0009014,GO:0008237 3.5.1.18 P9WHS9 NC_000962.3 Mycobrowser_v5 CDS 1349332 1351125 . + 0 Rv1206 fadD6 Function unknown, but supposed involvement in lipid degradation. Probable fatty-acid-CoA ligase FadD6 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv1206, (MTCI364.18), len: 597 aa. Probable fadD6, fatty-acid-CoA synthetase, highly similar to several e.g. NP_251583.1|NC_002516 probable very-long-chain acyl-CoA synthetase from Pseudomonas aeruginosa (608 aa); Q60714 mouse fatty acid transport protein fatp (646 aa), FASTA scores: opt:712, E(): 0, (36.8% identity in 600 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family. O05307 No lipid metabolism O05307 O05307 GO:0008152,GO:0016874 6.2.1.- O05307 NC_000962.3 Mycobrowser_v5 CDS 1355836 1357050 . + 0 Rv1213 glgC Involved in glycogen biosynthesis (first step) [catalytic activity:ATP + alpha-D-glucose 1-phosphate = diphosphate + ADP-glucose]. Glucose-1-phosphate adenylyltransferase GlgC (ADP-glucose synthase) (ADP-glucose pyrophosphorylase) Rv1213, (MTCI364.25), len: 404 aa. glgC, glucose-1-phosphate adenylyltransferase, similar to many e.g. GLGC_ECOLI|P00584 Escherichia coli (430 aa), FASTA scores: opt: 1075, E(): 0, (40.3% identity in 407 aa overlap); highly similar to Q49961 GLGC from Mycobacterium leprae (419 aa), FASTA scores: opt: 2532, E(): 0, (92.6% identity in 404 aa overlap). Belongs to the bacterial and plants glucose-1-phosphate adenylyltransferase family. P9WN43 No intermediary metabolism and respiration P64241 P9WN43 GO:0008878,GO:0005978,GO:0005524 2.7.7.27 P9WN43 NC_000962.3 Mycobrowser_v5 CDS 1360155 1361801 . - 0 Rv1217c Rv1217c Thought to be involved in active transport of tetronasin across the membrane (export): tetronasin resistance by an export mechanism. Responsible for the translocation of the substrate across the membrane. Probable tetronasin-transport integral membrane protein ABC transporter Rv1217c, (MTCI364.29c), len: 548 aa. Probable tetronasin-transport integral membrane ABC transporter (see citation below), similar to many e.g. AL049754|SCH10_12 probable ABC-type transport system membrane-spanning protein from Streptomyces coelicolor (539 aa), FASTA scores: opt: 1309, E(): 0, (40.9% identity in 550 aa overlap); Q54407|X73633 TnrB3 protein from Streptomyces longisporoflavus (337 aa), FASTA scores: opt: 692, E(): 0, (39.5% identity in 324 aa overlap); etc. Also has regions similar to Mycobacterium leprae proteins Q49964|U1756Q (109 aa), FASTA scores: opt: 431, E(): 3.1e-20, (64.8% identity in 105 aa overlap) and Q49965|U1756R (82 aa), FASTA scores: opt:154, E(): 0.0028, (61.0% identity in 41 aa overlap). O05318 No cell wall and cell processes O05318 O05318 NC_000962.3 Mycobrowser_v5 CDS 1364413 1365186 . + 0 Rv1221 sigE The sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released. Seems to be regulated by sigh (Rv3223c product). Seems to regulate the heat-shock response. Alternative RNA polymerase sigma factor SigE Rv1221, (MTCI61.04), len: 257 aa. SigE, alternative sigma factor of extracytoplasmic function (ECF) family (see citations below). Similar to many e.g. RPOE_HAEIN|P44790 RNA polymerase sigma-e factor from Haemophilus influenzae (189 aa), FASTA scores: opt: 247, E(): 3.4e-06, (28.5% identity in 186 aa overlap); etc. Also similar to MTCY07D11.03 rpoE from Mycobacterium tuberculosis (35.2% identity in 159 aa overlap). Belongs to the sigma-70 factor family, ECF subfamily. Three promoters and three translational start codons have been detected (See Dona et al., 2008). Fourth transcriptional start point has been identified (See Pang et al., 2007). Note that in Mycobacterium bovis BCG, the sigE gene is transcribed from two promoters, P1 and P2, and that these promoters were expressed at temperatures from 30-50 degrees Celsius. P9WGG7 No information pathways O06289 P9WGG7 GO:0016987,GO:0003700,GO:0006352,GO:0006355 P9WGG7 NC_000962.3 Mycobrowser_v5 CDS 1365875 1367461 . + 0 Rv1223 htrA Possibly hydrolyzes peptides and/or proteins (seems to cleave preferentially after serine residue). Probable serine protease HtrA (DEGP protein) Rv1223, (MTCI61.06), len: 528 aa. Probable htrA (alternate gene name: degP), serine protease precursor (see citations below), equivalent to U15180|MLU15180_31|Q49972|ML1078|HTRA possible serine protease from Mycobacterium leprae (533 aa), FASTA scores: opt: 2777, E(): 4.1e-141, (81.6% identity in 533 aa overlap). Also similar to many others e.g. HTRA_ECOLI|P09376 protease do precursor from Escherichia coli (474 aa), FASTA scores: opt: 581, E(): 9.1e-27, (36.3% identity in 278 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Start changed since first submission (-21 aa). Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). O06291 No intermediary metabolism and respiration O06291 O06291 GO:0006508,GO:0006950,GO:0004252,GO:0005515 3.4.21.- O06291 NC_000962.3 Mycobrowser_v5 CDS 1367891 1368721 . - 0 Rv1225c Rv1225c Function unknown Conserved hypothetical protein Rv1225c, (MTCI61.08c), len: 276 aa. Conserved hypothetical protein, some similarity to other hypothetical proteins e.g. AE001078|AE001078_2 Archaeoglobus fulgidus (265 aa), FASTA scores: opt: 339, E(): 5.1e-15, (27.1% identity in 262 aa overlap), and to NAGD_ECOLI|P15302 nagd protein from Escherichia coli (250 aa), FASTA scores: opt: 167, E(): 6.4e-12, (24.8% identity in 258 aa overlap). Also weakly similar to Mycobacterium tuberculosis hypothetical protein Rv3400|MTCY78.28c (29.1% identity in 251 aa overlap). O33221 No conserved hypotheticals O33221 O33221 GO:0008152,GO:0016787 O33221 NC_000962.3 Mycobrowser_v5 CDS 1374861 1376168 . - 0 Rv1232c Rv1232c Function unknown Conserved protein Rv1232c, (MTV006.04c), len: 435 aa. Conserved protein, similar to other hypothetical proteins e.g. AB013374|AB013374_2 Bacillus halodurans C-125 mamX (449 aa), FASTA scores: opt: 381, E(): 1e-16, (29.9% identity in 251 aa overlap). Some similarity in N-terminus to U15180|MLU1518033 hypothetical Mycobacterium leprae protein u1756u (329 aa), FASTA scores: opt: 300, E(): 4.1e-12, (69.3% identity in 75 aa overlap). O86315 No conserved hypotheticals O86315 O86315 O86315 NC_000962.3 Mycobrowser_v5 CDS 1377524 1378930 . + 0 Rv1235 lpqY Thought to be involved in active transport of sugar across the membrane (import). Probable sugar-binding lipoprotein LpqY Rv1235, (MTV006.07), len: 468 aa. Probable lpqY, sugar-binding lipoprotein component of sugar transport system (see citation below), equivalent to MLU1518034 protein u1756v from Mycobacterium leprae (469 aa), FASTA scores: opt: 2442, E(): 0, (77.4% identity in 470 aa overlap). Also similar to P18815|MALE_ENTAE maltose-binding periplasmic protein from Enterobacter aerogenes (396 aa), FASTA scores: opt: 193, E(): 2.3e-05, (24.2% identity in 297 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. P9WGU9 No cell wall and cell processes Q7D8J9 P9WGU9 GO:0005215,GO:0006810 P9WGU9 NC_000962.3 Mycobrowser_v5 CDS 1380684 1381865 . + 0 Rv1238 sugC Involved in active transport of sugar across the membrane (import). Responsible for energy coupling to the transport system. Probable sugar-transport ATP-binding protein ABC transporter SugC Rv1238, (MTV006.10), len: 393 aa. Probable sugC, sugar-transport ATP-binding protein ABC transporter (see citation below). Highly similar to U15180 protein ugpC from Mycobacterium leprae (392 aa), FASTA score: opt: 2007, E(): 0, (79.9% identity in 389 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). P9WQI3 No cell wall and cell processes O50454 P9WQI3 GO:0043190,GO:0016887,GO:0016820,GO:0006810,GO:0005524 P9WQI3 NC_000962.3 Mycobrowser_v5 CDS 1484279 1484959 . + 0 Rv1321 Rv1321 Function unknown Conserved hypothetical protein Rv1321, (MTCY130.06), len: 226 aa. Conserved hypothetical protein. Equivalent to P53524|YD21_MYCLE hypothetical protein from Mycobacterium leprae (201 aa), FASTA scores: opt: 1144, E(): 0, (87.6% identity in 193 aa overlap). Some similarity to hypothetical proteins from other organisms e.g. Y225_METJA|Q57678 Methanococcus jannaschii (263 aa), FASTA scores: E(): 6.5e-05, (25.0% identity in 212 aa overlap). P9WIY5 No conserved hypotheticals Q10634 P9WIY5 P9WIY5 NC_000962.3 Mycobrowser_v5 CDS 1387798 1388628 . - 0 Rv1245c Rv1245c Function unknown; supposedly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv1245c, (MTV006.17c), len: 276 aa. Probable short-chain dehydrogenase/reductase, equivalent to NP_301801.1|NC_002677 short chain alcohol dehydrogenase from Mycobacterium leprae (277 aa). Also highly similar to various dehydrogenases and oxidoreductases e.g. NP_250228.1|NC_002516 probable short-chain dehydrogenase from Pseudomonas aeruginosa (295 aa); NP_421969.1|NC_002696 short chain dehydrogenase family protein from Caulobacter crescentus (278 aa); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. Rv3085|MTV013.06 probable short-chain type dehydrogenase/reductase (276 aa), FASTA scores: opt: 368, E(): 1.2e-16, (35.3% identity in 224 aa overlap); Rv3057c|MTCY22D7.24 putative short chain alcohol dehydrogenase/reductase (287 aa), FASTA scores: opt: 471, E(): 1.3e-21, (32.4% identity in 281 aa overlap); etc. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. O50460 No intermediary metabolism and respiration O50460 O50460 GO:0055114,GO:0016491 1.-.-.- O50460 NC_000962.3 Mycobrowser_v5 CDS 1389357 1393052 . - 0 Rv1248c Rv1248c Involved in cellular metabolism. Has alpha-ketoglutarate dehydrogenase (KDH) [catalytic activity: 2-oxoglutarate + lipoamide = S-succinyldihydrolipoamide + CO2], alpha-ketoglutarate decarboxylase (KGD) [catalytic activity: 2-oxoglutarate = succinate semialdehyde + CO2], and 2-hydroxy-3-oxoadipate (HOA) synthase [catalytic activity: 2-oxoglutarate + glyoxylate + H+ = 2-hydroxy-3-oxoadipate + CO2] activities. Multifunctional alpha-ketoglutarate metabolic enzyme Rv1248c, (MTV006.20c), len: 1231 aa. Multifunctional alpha-ketoglutarate metabolic enzyme, highly similar to D84102 Corynebacterium glutamicum (1257 aa), FASTA scores: opt: 4418, E(): 0, (59.4% identity in 1223 aa overlap). Cofactor: thiamine diphosphate. Start changed since first submission (+17 aa). P9WIS5 No intermediary metabolism and respiration O50463 P9WIS5 GO:0008415,GO:0006096,GO:0004591,GO:0030976,GO:0006099,GO:0008683 4.1.1.71 P9WIS5 NC_000962.3 Mycobrowser_v5 CDS 1406081 1407340 . - 0 Rv1258c Rv1258c Thought to be involved in transport of undetermined substrate (possibly macrolide) across the membrane (export). Responsible for the translocation of the undetermined substrate across the membrane. Probable conserved integral membrane transport protein Rv1258c, MTCY50.24, len: 419 aa. Probable conserved integral membrane transport (efflux) protein, possibly member of major facilitator superfamily (MFS), highly similar to O32859|tap protein multidrug-resistance efflux pump from Mycobacterium fortuitum (409 aa), FASTA scores: E(): 0, (68.4% identity in 408 aa overlap). Contains PS00216 Sugar transport proteins signature 1. P9WJX9 No cell wall and cell processes P64783 P9WJX9 GO:0005886,GO:0046677,GO:0055085,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1408240 1409358 . + 0 Rv1260 Rv1260 Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv1260, (MTCY50.22c), len: 372 aa. Probable oxidoreductase, highly similar to E1245747|AL021411 putative oxidoreductase SC7H1.18 from Streptomyces coelicolor (397 aa), FASTA scores: E(): 1.4e-29, (45.9% identity in 355 aa overlap); also some similarity to G912582 FAD binding protein homologue from Pseudomonas aeruginosa (286 aa), FASTA scores: opt: 245, E(): 2e-09, (27.5% identity in 251 aa overlap); PCPB_FLASP|P42535 pentachlorophenol 4-monooxygenase (537 aa), FASTA scores: opt: 219, E(): 1.7e-07, (23.3% identity in 360 aa overlap); TETX_BACFR|Q01911 tetracycline resistance protein (388 aa), FASTA scores: opt: 183, E(): 3e-05, (22.8% identity in 373 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins Rv0575c and Rv1751. P9WM51 No intermediary metabolism and respiration Q11058 P9WM51 GO:0055114,GO:0004497 P9WM51 NC_000962.3 Mycobrowser_v5 CDS 1411894 1413087 . + 0 Rv1264 Rv1264 Possibly involved in cAMP synthesis [catalytic activity: ATP = 3',5'-cyclic AMP + diphosphate]. Activity is PH-dependent. Inhibited by polyphosphates. Adenylyl cyclase (ATP pyrophosphate-lyase) (adenylate cyclase) Rv1264, (MTCY50.18c), len: 397 aa. Adenylate cyclase (function proven experimentally: see Linder et al., 2002), showing some similarity to other adenylate cyclases e.g. CYAA_BRELI|P27580 (403 aa), FASTA scores, opt: 270, E(): 1.3e-10, (29.3% identity in 317 aa overlap); etc. Similar to other putative cyclases in M. tuberculosis e.g. Rv2212, Rv1647. The C terminus seems to code for a catalytic domain belonging to a subfamily of adenylyl cyclase isozymes (mostly found in Gram-positive bacteria). The N terminus seems to be a potential novel regulator of adenylyl cyclase activity (autoinhibitory domain). Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. P9WMU9 No intermediary metabolism and respiration 1Y10,1Y11,2EV1,2EV2,2EV3,2EV4 Q11055 P9WMU9 GO:0004016,GO:0006171,GO:0008289,GO:0046872,GO:0030818,GO:0042803,GO:0005524 4.6.1.1 P9WMU9 NC_000962.3 Mycobrowser_v5 CDS 1484982 1485278 . + 0 Rv1322 Rv1322 Function unknown Conserved hypothetical protein Rv1322, (MTCY130.07), len: 98 aa. Conserved hypothetical protein. P9WM27 No conserved hypotheticals P9WM27 NC_000962.3 Mycobrowser_v5 CDS 1422302 1424050 . - 0 Rv1273c Rv1273c Thought to be involved in active transport of drugs across the membrane (export): multidrugs resistance by an export mechanism. Responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane. Probable drugs-transport transmembrane ATP-binding protein ABC transporter Rv1273c, (MTCY50.09), len: 582 aa. Probable drugs-transport transmembrane ATP-binding protein ABC transporter (see citation below), similar to e.g. YWJA_BACSU|P45861 hypothetical abc transporter from B. subtilis (575 aa), FASTA scores: opt: 810, E(): 0, (27.0% identity in 578 aa overlap); etc. Contains PS00136 Serine proteases, subtilase family, aspartic acid active site; 2 x PS00211 ABC transporters family signature; and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters), MSBA subfamily. P9WQJ1 No cell wall and cell processes P0A4W4 P9WQJ1 GO:0042626,GO:0016021,GO:0005886,GO:0055085,GO:0005524 P9WQJ1 NC_000962.3 Mycobrowser_v5 CDS 1431665 1433440 . - 0 Rv1280c oppA Involved in active transport of oligopeptide across the membrane (import). This protein is a component of the oligopeptide permease, a binding protein-dependent transport system; it binds peptides up to five amino acids long with high affinity. Probable periplasmic oligopeptide-binding lipoprotein OppA Rv1280c, (MTCY50.02), len: 591 aa. Probable oppA, oligopeptide-binding lipoprotein component of peptide transport system (see citation below), sharing some similarity to other periplasmic solute binding proteins e.g. OPPA_SALTY|P06202 periplasmic oligopeptide-binding protein from Salmonella typhimurium (542 aa), FASTA scores: E(): 5.1e-05, (22.1% identity in 458 aa overlap); etc. Also similar to Rv1166 and Rv2585c from Mycobacterium tuberculosis. Has possible N-terminal signal sequence and prokaryotic lipoprotein lipid attachment site (PS00013). Belongs to the bacterial extracellular solute-binding protein family 5. P9WGU5 No cell wall and cell processes P66771 P9WGU5 GO:0005886,GO:0006810,GO:0005215,GO:0016021 P9WGU5 NC_000962.3 Mycobrowser_v5 CDS 1437909 1438907 . + 0 Rv1285 cysD Involved in sulfate activation pathway. First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway [catalytic activity:ATP + sulfate = diphosphate + adenylylsulfate] Probable sulfate adenylyltransferase subunit 2 CysD Rv1285, (MTCY373.04), len: 332 aa. Probable cysD, sulfate adenylyltransferase subunit 2 (see Wooff et al., 2002), homology suggests start site at aa 24 or 28, similar to e.g. CYSD_ECOLI|P21156 sulfate adenylate transferase subunit 2 from Escherichia coli (302 aa), FASTA score: opt: 973, E():0, (52.5% identity in 303 aa overlap). Also similar to Mycobacterium tuberculosis Rv2392, 3'-phosphoadenylylsulfate reductase. Belongs to the PAPS reductase family. CYSD subfamily. Thought to be differentially expressed within host cells (see Triccas et al., 1999). P9WIK1 No intermediary metabolism and respiration P65670 P9WIK1 GO:0004781,GO:0000103,GO:0019419,GO:0005524 2.7.7.4 P9WIK1 NC_000962.3 Mycobrowser_v5 CDS 2413348 2414922 . - 0 Rv2154c ftsW Unknown FtsW-like protein FtsW Rv2154c, (MTCY270.14), len: 524 aa. Probable ftsW, cell division protein, related to MTCY10H4.17c, 3.2e-17. FASTA best: SP5E_BACSU P07373 stage V sporulation protein E (366 aa) opt: 755, E(): 1.6e-33; (38.4% identity in 357 aa overlap). FtsW|Rv2154c interacts with PbpB|Rv2163c and FtsZ|RvRv2150c (See Datta et al., 2006). P9WN97 No cell wall and cell processes P63762 P9WN97 GO:0051301,GO:0016021,GO:0009252,GO:0005886,GO:0007049 NC_000962.3 Mycobrowser_v5 CDS 2900226 2900690 . - 0 Rv2576c Rv2576c Unknown Possible conserved membrane protein Rv2576c, (MTCY227.25), len: 154 aa. Possible conserved membrane protein, showing similarity with Q9ZFC2 hypothetical 15.7 KDA protein from Mycobacterium sp. FM10 (146 aa), FASTA scores: opt: 235, E(): 4.1e-08, (31.35% identity in 150 aa overlap). P9WL83 No cell wall and cell processes P9WL83 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1449375 1450700 . + 0 Rv1294 thrA Involved in the conversion of L-aspartate to homoserine (third step). Homoserine participates in the biosynthesis of threonine and then isoleucine and in the biosynthesis of methionine [catalytic activity: L-homoserine + NAD(P)(+) = L-aspartate 4-semialdehyde + NAD(P)H.] Probable homoserine dehydrogenase ThrA Rv1294, (MTCY373.14), len: 441 aa. Probable thrA (hom), homoserine dehydrogenase, highly similar to DHOM_MYCLE|P46806 from Mycobacterium leprae (441 aa), FASTA scores: opt: 2437, E():0, (89.5% identity in 438 aa overlap). Contains PS00017 ATP/GTP-binding site motif A; PS01042 Homoserine dehydrogenase signature. Belongs to the homoserine dehydrogenase family. P9WPX1 No intermediary metabolism and respiration P63629 P9WPX1 GO:0016597,GO:0004412,GO:0009097,GO:0009086,GO:0055114,GO:0009088,GO:0050661 1.1.1.3 P9WPX1 NC_000962.3 Mycobrowser_v5 CDS 1453204 1455012 . + 0 Rv1297 rho Facilitates transcription termination by a mechanism that involves rho binding to the nascent RNA, activation of rho'S RNA-dependent ATPase activity, and release of the mRNA from the DNA template Probable transcription termination factor Rho homolog Rv1297, (MTCY373.17), len: 602 aa. Probable rho, transcription termination factor homolog, highly similar to many e.g. RHO_MYCLE|P45835 Mycobacterium leprae (610 aa), FASTA scores: (81.5% identity in 612 aa overlap). Contains 1 RNA recognition motif (RRM). Nucleotide position 1453608 in the genome sequence has been corrected, T:C resulting in G135G. P9WHF3 No information pathways P66028 P9WHF3 GO:0015986,GO:0003723,GO:0004386,GO:0016820,GO:0033178,GO:0045449,GO:0006353,GO:0003715,GO:0005524 3.6.4.- P9WHF3 NC_000962.3 Mycobrowser_v5 CDS 1458295 1459509 . + 0 Rv1302 rfe Thought to be involved in AG biosynthesis. May be the tunicamycin sensitive transferase that catalyzes the synthesis of GlcNAc-pyrophosphorylundecaprenol (lipid I), the first lipid-linked intermediate involved in ECA synthesis. Probable undecapaprenyl-phosphate alpha-N-acetylglucosaminyltransferase Rfe (UDP-GlcNAc transferase) Rv1302, (MTCY373.22), len: 404 aa. Probable rfe (alternate gene name: wecA), undecaprenyl-phosphate alpha-N-acetylglucosaminyltransferase (see citation below), equivalent to RFE_MYCLE|P45830 Mycobacterium leprae (398 aa), FASTA scores, opt: 2285, E(): 0, (89.2% identity in 398 aa overlap). P9WMW5 No cell wall and cell processes Q10606 P9WMW5 GO:0009103,GO:0008963,GO:0005886,GO:0016757,GO:0016021 2.7.8.n2 P9WMW5 NC_000962.3 Mycobrowser_v5 CDS 1461843 1463183 . + 0 Rv1307 atpH This protein seems to be part of the stalk that LINKS cf(0) to cf(1). It either transmits conformational changes from cf(0) into cf(1) or is implicated in proton conduction [catalytic activity: ATP + H(2)O + H(+)(in) = ADP + phosphate + H(+)(out)] Probable ATP synthase delta chain AtpH Rv1307, (MTCY373.27), len: 446 aa. Probable atpH, ATP synthase delta chain. This protein is much longer than that of other bacterial delta chains, the C-terminal region is homologous to delta chains while the N-terminal region is similar to B/B' subunits e.g. ATPD_STRLI|P50008 ATP synthase delta chain from Streptomyces lividans (273 aa), FASTA scores: opt: 505, E(): 5.4e-23, (35.0% identity in 277 aa overlap); and ATPF_HAEIN|P43720 ATP synthase B chain from Haemophilus influenzae (156 aa), FASTA scores: opt: 216, E(): 1.2e-06, (26.1% identity in 153 aa overlap). subunit: F-type ATPases have 2 components, cf(1) - the catalytic core - and cf(0) - the membrane proton channel. cf(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). cf(0) has three main subunits: A, B and C. Belongs to the ATPase delta chain family. P9WPV3 No intermediary metabolism and respiration P0A500 P9WPV3 GO:0003824,GO:0046933,GO:0016021,GO:0005886,GO:0045261,GO:0045263,GO:0015986 P9WPV3 NC_000962.3 Mycobrowser_v5 CDS 1463228 1464877 . + 0 Rv1308 atpA Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit [catalytic activity:ATP + H(2)O + H(+)(in) = ADP + phosphate + H(+)(out)] Probable ATP synthase alpha chain AtpA Rv1308, (MTCY373.28), len: 549 aa. Probable atpA, ATP synthase alpha chain, highly similar to ATPA_MYCLE|P45825 from Mycobacterium leprae (558 aa), FASTA scores: opt: 3233, E(): 0, (90.3% identity in 547 aa overlap). Contains PS00017 ATP/GTP-binding site motif A, PS00152 ATP synthase alpha and beta subunits signature. subunit: F-type ATPases have 2 components, cf(1) - the catalytic core - and cf(0) - the membrane proton channel. cf(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). cf(0) has three main subunits: A, B and C. Belongs to the ATPase alpha/beta chains family. P9WPU7 No intermediary metabolism and respiration P63673 P9WPU7 GO:0046933,GO:0005886,GO:0042777,GO:0045261,GO:0046961,GO:0005524 3.6.3.14 P9WPU7 NC_000962.3 Mycobrowser_v5 CDS 1468171 1469505 . - 0 Rv1313c Rv1313c Possibly required for the transposition of the insertion element IS1557. Possible transposase Rv1313c, (MTCY373.33c), len: 444 aa. Possible IS1557 transposase, similar to several transposases e.g. U57649|DBU57649 ORF1 from dibenzofuran-degrading bacterium DPO360 (163 aa), FASTA scores: opt: 767, E(): 0, (67.3% identity in 168 aa overlap); TNPA_BORPA|Q06126 transposase for insertion sequence element IS1001 from Bordetella parapertussis (406 aa), FASTA scores: opt: 254, E(): 3.3e-10, (24.9% identity in 402 aa overlap). Also similar to putative Mycobacterium tuberculosis transposases, Rv3798 and Rv0741. P9WKH7 No insertion seqs and phages Q10621 P9WKH7 GO:0004803,GO:0006313,GO:0003677 P9WKH7 NC_000962.3 Mycobrowser_v5 CDS 1477628 1479118 . - 0 Rv1317c alkA Involved in the adaptive response to alkylation damage in DNA caused by alkylating agents. Repairs the SP diastereomer of DNA methylphosphotriester lesions by a direct and irreversible transfer of the methyl group to one of its own cysteine residues. The methylated ALKA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. Probable bifunctional regulatory protein and DNA repair enzyme AlkA (regulatory protein of adaptative response) (methylphosphotriester-DNA--protein-cysteine S-methyltransferase) Rv1317c, (MTCY130.02c), len: 496 aa. Probable alkA (alternate gene name: ada), bifunctional regulatory protein (see citation below) and DNA repair enzyme, similar to 3MG2_ECOLI|P04395 dna-3-methyladenine glycosidase II from Escherichia coli (282 aa), FASTA scores, opt: 437, E(): 8.6e-22, (32.8% identity in 293 aa overlap), also similar to other ada proteins e.g. ADA_SALTY|P26189 Salmonella typhimurium (352 aa), FASTA scores: E(): 5.3e-08, (35.9% identity in 156 aa overlap). Contains PS00041 Bacterial regulatory proteins, araC family signature. P9WJW3 No information pathways Q10630 P9WJW3 GO:0006284,GO:0003908,GO:0006355,GO:0006350,GO:0008270,GO:0003677 P9WJW3 NC_000962.3 Mycobrowser_v5 CDS 1480894 1482501 . - 0 Rv1319c Rv1319c Thought to play an essential role in regulation of cellular metabolism by catalysing the synthesis of a second messenger, camp [catalytic activity: ATP = 3',5'-cyclic AMP + pyrophosphate]. Possible adenylate cyclase (ATP pyrophosphate-lyase) (adenylyl cyclase) Rv1319c, (MTCY130.04c), len: 535 aa. Possible adenylate cyclase. Some similarity at the C-terminus to CYAA_RHIME|P19485 adenylate cyclase from Rhizobium meliloti (193 aa), FASTA scores: opt: 254, E(): 2.4e-10, (33.3% identity in 144 aa overlap); similar to other mycbacterium tuberculosis putative adenylate cyclases e.g. Rv1318c|MTCY130.03c (541 aa), FASTA scores: opt: 2505, E(): 0, (71.0% identity in 534 aa overlap); Rv1320c|MTCY130.05c (567 aa), FASTA scores: opt: 2354, E(): 0, (66.3% identity in 534 aa overlap). N-terminus is hydrophobic. Belongs to adenylyl cyclase class-3 family. P9WQ31 No intermediary metabolism and respiration P0A4Y2 P9WQ31 GO:0016021,GO:0016849,GO:0005886,GO:0004871,GO:0007165,GO:0009190 P9WQ31 NC_000962.3 Mycobrowser_v5 CDS 1488154 1489965 . - 0 Rv1325c PE_PGRS24 Function unknown PE-PGRS family protein PE_PGRS24 Rv1325c, (MTCY130.10c), len: 603 aa. PE_PGRS24, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of ala-, gly-rich proteins (see Brennan & Delogu 2002), similar to many e.g. YQ04_MYCTU|P71933 hypothetical 63.1 kDa glycine-rich protein (778 aa), FASTA scores: E(): 0, (52.3% identity in 724 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). P9WIF7 No PE/PPE Q10637 P9WIF7 P9WIF7 NC_000962.3 Mycobrowser_v5 CDS 1499213 1500559 . - 0 Rv1330c pncB1 Involved in NAD salvage. Phosphoribosylation of nicotinic acid. [catalytic activity: nicotinate + 5-phosphoribosyl 1-pyrophosphate = nicotinate mononucleotide + diphosphate] Nicotinic acid phosphoribosyltransferase PncB1 Rv1330c, (MTCY130.15c), len: 448 aa. PncB1, nicotinic acid phosphoribosyltransferase (See Boshoff et al., 2008). Similar to e.g. O32090 YUEK protein from Bacillus subtilis (490 aa), FASTA scores: E(): 8.6e-22, (37.9% identity in 369 aa overlap). Also similar to Mycobacterium tuberculosis Rv0573c|MTV039.11c (38.0% identity in 437 aa overlap). Start changed since original submission based on similarity; previous start at position 1500740 (-61 aa). P9WJI9 No intermediary metabolism and respiration Q10641 P9WJI9 GO:0019363,GO:0004516 2.4.2.11 P9WJI9 NC_000962.3 Mycobrowser_v5 CDS 1509281 1510846 . + 0 Rv1345 mbtM Thought to be involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins Probable fatty acyl-AMP ligase MbtM Rv1345, (MTCY02B10.09), len: 521 aa. Probable mbtM, fatty acyl-AMP ligase. Similar to N-terminus of T34918 polyketide synthase from Streptomyces coelicolor (2297 aa); and PKSJ_BACSU|P40806 putative polyketide biosynthesis protein from Bacillus subtilis (557 aa), FASTA scores: opt: 537, E(): 8.2e-27, (27.1% identity in 468 aa overlap). Also similar to other proteins from Mycobacterium tuberculosis eg Rv1013|MTCI237.30|MTCY10G2.36c|pks16 putative polyketide synthase (544 aa); etc. Note that previously known as fadD33. P9WQ41 No lipid metabolism P0A4X8 P9WQ41 GO:0006826,GO:0008922,GO:0008152,GO:0005524 6.2.1.20 P9WQ41 NC_000962.3 Mycobrowser_v5 CDS 1513047 1515626 . + 0 Rv1348 irtA Involved in iron homeostasis. Responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane. Iron-regulated transporter IrtA Rv1348, (MTCY02B10.12), len: 859 aa. IrtA, iron-regulated transporter. Probable transmembrane protein, similar to HMT1_SCHPO|Q02592 heavy metal tolerance protein precursor from Schizosaccharomyces pombe (830 aa), FASTA scores: opt: 806, E(): 5.1e-39, (32.9% identity in 504 aa overlap); etc. Also similar to MTCY02B10.13 from Mycobacterium tuberculosis, FASTA score: (31.9% identity in 576 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). Cofactor: FAD P9WQJ9 No cell wall and cell processes P63391 P9WQJ9 GO:0042626,GO:0016021,GO:0055114,GO:0016491,GO:0005886,GO:0055085,GO:0005524 P9WQJ9 NC_000962.3 Mycobrowser_v5 CDS 1515623 1517362 . + 0 Rv1349 irtB Involved in iron homeostasis. Responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane. Iron-regulated transporter IrtB Rv1349, (MTCY02B10.13), len: 579 aa. IrtB, iron-regulated transporter. Probable transmembrane protein, most similar to YWJA_BACSU|P45861 hypothetical ABC transporter from Bacillus subtilis (575 aa), FASTA scores: opt: 721, E(): 1.8e-35, (28.9% identity in 567 aa overlap); etc. Also similar to MTCY02B10.12 from Mycobacterium tuberculosis, FASTA score: (31.9% identity in 576 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). Predicted possible vaccine candidate (See Zvi et al., 2008). P9WQJ7 No cell wall and cell processes P63393 P9WQJ7 GO:0042626,GO:0016021,GO:0005886,GO:0055085,GO:0005524 P9WQJ7 NC_000962.3 Mycobrowser_v5 CDS 1520005 1521876 . - 0 Rv1354c Rv1354c Function unknown Conserved hypothetical protein Rv1354c, (MTCY02B10.18c), len: 623 aa. Conserved hypothetical protein, similar to many hypothetical proteins e.g. the C-terminus of G1001455 Synechocystis sp. (1244 aa), FASTA scores: opt: 933, E(): 0, (36.8% identity in 462 aa overlap); also similar to Rv1357c|MTCY02B10.21c (34.0% identity in 253 aa overlap). P9WM13 No conserved hypotheticals P64825 P9WM13 P9WM13 NC_000962.3 Mycobrowser_v5 CDS 1526612 1530091 . + 0 Rv1358 Rv1358 Involved in transcriptional mechanism Probable transcriptional regulatory protein Rv1358, (MTCY02B10.22), len: 1159 aa. Probable transcriptional regulatory protein, some similarity to AFSR_STRCO|P25941 regulatory protein afsr from Streptomyces coelicolor (993 aa), FASTA scores: opt: 210, E(): 5.5e-06, (27.5% identity in 739 aa overlap). Similar also to Rv0890C|MTCY31.18c (65.5% identity in 884 aa overlap) and to Rv1359|MTCY02B10.23 (43.7% identity in 197 aa overlap). Contains PS00017 ATP/GTP-binding site motif A, PS00622 Bacterial regulatory proteins, luxR family signature. Helix turn helix motif present at aa 1116-1137, (Score 1291, +3.59 SD). Q11028 No regulatory proteins Q11028 Q11028 GO:0005622,GO:0016849,GO:0006355,GO:0043565,GO:0003700,GO:0000156,GO:0000160,GO:0009190 Q11028 NC_000962.3 Mycobrowser_v5 CDS 1541020 1541805 . + 0 Rv1368 lprF Unknown Probable conserved lipoprotein LprF Rv1368, (MTCY02B12.02), len: 261 aa. Probable lprF, conserved lipoprotein; similar to Mycobacterium tuberculosis hypothetical lipoproteins e.g. Rv1270c|Y08C_MYCTU|Q11049 hypothetical 26.4 kDa protein cy50.12. (257 aa), FASTA scores: opt: 286, E(): 5.3e-11, (26.3% identity in 270 aa overlap), also Rv1411c|MTCY21B4.28c, (32.8% identity in 253 aa overlap) and Rv2945c. Contains possible N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein lipid attachment site (PS00013). Belongs to the LPPX/lprafg family of lipoproteins. P9WK47 No cell wall and cell processes P65314 P9WK47 GO:0005886 P9WK47 NC_000962.3 Mycobrowser_v5 CDS 1550579 1551217 . - 0 Rv1377c Rv1377c Function unknown; probably involved in cellular metabolism Putative transferase Rv1377c, (MTCY02B12.11c), len: 212 aa. Putative transferase, similar to YQEM_BACSU|P54458 hypothetical 28.3 kDa protein from Bacillus subtilis (247 aa), FASTA scores: opt: 221, E(): 7.6e-08, (30.6% identity in 144 aa overlap); some similarity to methyltransferases, also similar to Mycobacterium tuberculosis hypothetical proteins Rv0560c, Rv3699, and Rv2675c (~ 39.1% identity in 197 aa overlap). P71805 No intermediary metabolism and respiration P71805 P71805 GO:0008168,GO:0008152 2.-.-.- P71805 NC_000962.3 Mycobrowser_v5 CDS 1551228 1552655 . - 0 Rv1378c Rv1378c Function unknown Conserved hypothetical protein Rv1378c, (MTCY02B12.12c), len: 475 aa. Conserved hypothetical protein, similar to other Mycobacterium tuberculosis hypothetical proteins e.g. Rv3074|MTCY22D7.07C (424 aa), FASTA scores: E(): 0, (73.0% identity in 429 aa overlap). P71806 No conserved hypotheticals P71806 NC_000962.3 Mycobrowser_v5 CDS 1553232 1554191 . + 0 Rv1380 pyrB Involved in pyrimidine biosynthesis (second step) [catalytic activity: carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate] Probable aspartate carbamoyltransferase PyrB (ATCase) (aspartate transcarbamylase) Rv1380, (MTCY02B12.14), len: 319 aa. Probable pyrB, aspartate carbamoyltransferase, similar to many e.g. PYRB_BACCL|P41008 aspartate carbamoyltransferase from Bacillus caldolyticus (308 aa), FASTA scores, opt: 639, E(): 7.3e-36, (39.5% identity in 311 aa overlap). Contains PS00097 Aspartate and ornithine carbamoyltransferases signature. Belongs to the ATCases/OTCases family. P9WIT7 No intermediary metabolism and respiration P65613 P9WIT7 GO:0016597,GO:0004070,GO:0006520,GO:0006221,GO:0006207 2.1.3.2 P9WIT7 NC_000962.3 Mycobrowser_v5 CDS 1555971 1557101 . + 0 Rv1383 carA Involved in both arginine and pyrimidine biosynthesis [catalytic activity: 2 ATP + L-glutamine + CO(2) + H(2)O = 2 ADP + phosphate + glutamate + carbamoyl phosphate] Probable carbamoyl-phosphate synthase small chain CarA (carbamoyl-phosphate synthetase glutamine chain) Rv1383, (MTCY02B12.17), len: 376 aa. Probable carA, Carbamoyl-phosphate synthase small chain, similar to many e.g. CARA_ECOLI|P00907 carbamoyl-phosphate synthase small chain from Escherichia coli (382 aa), FASTA scores: opt: 796, E(): 0, (45.5% identity in 382 aa overlap). Contains PS00442 Glutamine amidotransferases class-I active site. The gatase domain belongs to type-1 glutamine amidotransferases. subunit: composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. P9WPK5 No intermediary metabolism and respiration P71811 P9WPK5 GO:0006526,GO:0004088,GO:0006541,GO:0006221,GO:0005524 6.3.5.5 P9WPK5 NC_000962.3 Mycobrowser_v5 CDS 1557101 1560448 . + 0 Rv1384 carB Involved in both arginine and pyrimidine biosynthesis [catalytic activity: 2 ATP + L-glutamine + CO(2) + H(2)O = 2 ADP + phosphate + glutamate + carbamoyl phosphate.] Probable carbamoyl-phosphate synthase large chain CarB (carbamoyl-phosphate synthetase ammonia chain) Rv1384, (MTCY02B12.18-MTCY21B4.01), len: 1115 aa. Probable carB, Carbamoyl-phosphate synthase large chain , similar to many e.g. CARB_ECOLI|P00968 E. coli (1072 aa), FASTA scores: E(): 0, (52.3% identity in 1118 aa overlap). Contains two PS00867 Carbamoyl-phosphate synthase subdomain signature 2 and PS00866 Carbamoyl-phosphatesynthase subdomain signature 1. subunit: composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. P9WPK3 No intermediary metabolism and respiration P57689 P9WPK3 GO:0006526,GO:0004088,GO:0046872,GO:0006221,GO:0005524 6.3.5.5 P9WPK3 NC_000962.3 Mycobrowser_v5 CDS 1561769 1563388 . + 0 Rv1387 PPE20 Function unknown PPE family protein PPE20 Rv1387, (MTCY21B4.04), len: 539 aa. PPE20, Member of Mycobacterium tuberculosis PPE family of proteins, similar to many e.g. Y05F_MYCTU|Q10892 hypothetical 46.9 kd protein cy251.15 (463 aa), FASTA scores: E(): 4.2e-26, (37.7% identity in 531 aa overlap); similar also to MTCY274.23c (37.5% identity in 168 aa overlap). Contains PS00343 Gram-positive cocci surface proteins 'anchoring' hexapeptide. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WI23 No PE/PPE Q7D8H6 P9WI23 P9WI23 NC_000962.3 Mycobrowser_v5 CDS 1565441 1566697 . + 0 Rv1391 dfp Flavoprotein affecting synthesis of DNA and pantothenate metabolism Probable DNA/pantothenate metabolism flavoprotein homolog Dfp Rv1391, (MTCY21B4.08), len: 418 aa. Probable dfp, DNA/pantothenate metabolism flavoprotein homolog, similar to many e.g. DFP_ECOLI|P24285 Escherichia coli (430 aa), FASTA scores: opt: 763, E(): 0, (40.2% identity in 408 aa overlap). P9WNZ1 No intermediary metabolism and respiration P67733 P9WNZ1 GO:0015937,GO:0015941,GO:0004632,GO:0004633,GO:0010181 6.3.2.5,4.1.1.36 P9WNZ1 NC_000962.3 Mycobrowser_v5 CDS 1566825 1568036 . + 0 Rv1392 metK Involved in the activated methyl cycle. Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme. [catalytic activity : ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine] Probable S-adenosylmethionine synthetase MetK (mat) (AdoMet synthetase) (methionine adenosyltransferase) Rv1392, (MTCY21B4.09), len: 403 aa. Probable metK, S-adenosylmethionine synthetase, similar to many e.g. METK_STAAU|P50307 Staphylococcus aureus (397 aa), FASTA scores: opt: 1484, E(): 0, (58.0% identity in 400 aa overlap). Contains PS00376 S-adenosylmethionine synthetase signature 1, PS00377 S-adenosylmethionine synthetase signature 2. Belongs to the adomet synthetase family. P9WGV1 No intermediary metabolism and respiration 3TDE P77899 P9WGV1 GO:0005737,GO:0000287,GO:0004478,GO:0006730,GO:0005524 2.5.1.6 P9WGV1 NC_000962.3 Mycobrowser_v5 CDS 1571047 1572081 . + 0 Rv1395 Rv1395 Involved in transcriptional mechanism Transcriptional regulatory protein Rv1395, (MTCY21B4.12), len: 344 aa. Transcriptional regulatory protein (see citation below), similar to many e.g. URER_PROMI|Q02458 urease operon transcriptional activator from Proteus mirabilis (293 aa), FASTA scores: E():1.5e-08, (41.7% identity in 84 aa overlap); YHIX_ECOLI|P37639 hypothetical transcriptional regulatory protein from Escherichia coli (274 aa), FASTA scores: opt: 238, E(): 3.5e-09, (27.3% identity in 249 aa overlap); and G296916|X68281 possible virulence-regulating protein from Mycobacterium tuberculosis (339 aa), FASTA scores: opt: 228, E(): 1.9e-08, (27.0% identity in 278 aa overlap). Helix turn helix motif present, aa 261-282 (+4.68 SD). Belongs to the AraC/XylS family of transcriptional regulators. 3' part corrected since first submission (-14 aa). Experimentally shown to induce the expression of the cytochrome P450 gene (Rv1394c) and represses its own transcription. P9WMJ1 No regulatory proteins P68913 P9WMJ1 GO:0006355,GO:0043565,GO:0006350,GO:0003700,GO:0005622 P9WMJ1 NC_000962.3 Mycobrowser_v5 CDS 2752848 2752994 . - 0 Rv2452c Rv2452c Unknown Hypothetical protein Rv2452c, (MTV008.08c), len: 48 aa. Hypothetical unknown protein (see citation below). O53179 No conserved hypotheticals O53179 NC_000962.3 Mycobrowser_v5 CDS 1579598 1580422 . - 0 Rv1403c Rv1403c Causes methylation Putative methyltransferase Rv1403c, (MTCY21B4.20c), len: 274 aa. Putative methyltransferase, similar to PMTA_RHOSH|Q05197 phosphatidylethanolamine m-methyltransferase (203 aa), FASTA scores: opt: 217, E(): 1.1e-07, (37.1% identity in 105 aa overlap); similar to Rv1405c|MTCY21B4.22c (59.3% identity in 273 aa overlap) and to Rv1523, Rv2952, etc. P9WLY9 No intermediary metabolism and respiration P64839 P9WLY9 GO:0008168,GO:0008152 P9WLY9 NC_000962.3 Mycobrowser_v5 CDS 1582166 1583104 . + 0 Rv1406 fmt Modify the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTU-GTP. [catalytic activity: 10-formyltetrahydrofolate + L-methionyl-tRNA + H(2)O = tetrahydrofolate + N-formylmethionyl-tRNA] Probable methionyl-tRNA formyltransferase Fmt Rv1406, (MTCY21B4.23), len: 312 aa. Probable fmt, methionyl-tRNA formyltransferase, similar to many e.g. FMT_ECOLI|P23882 Escherichia coli (314 aa), FASTA scores: opt: 616, E(): 6.7e-31, (39.3% identity in 303 aa overlap). Belongs to the FMT family. P9WND3 No information pathways P64134 P9WND3 GO:0006412,GO:0004479 2.1.2.9 P9WND3 NC_000962.3 Mycobrowser_v5 CDS 1585194 1586213 . + 0 Rv1409 ribG Involved in riboflavin biosynthesis (at the second and third steps). Converts 2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate into 5-amino-6-(ribosylamino)-2,4(1H,3H)-pyrimidinedione 5'-phosphate [catalytic activity 1: 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + H(2)O = 5-amino-6-(5-phosphoribosylamino)uracil + NH(3)] [catalytic activity 2: 5-amino-6-(5-phosphoribitylamino)uracil + NADP(+) = 5-amino-6-(5-phosphoribosylamino)uracil + NADPH]. Probable bifunctional riboflavin biosynthesis protein RibG : diaminohydroxyphosphoribosylaminopyrimidine deaminase (riboflavin-specific deaminase) + 5-amino-6-(5-phosphoribosylamino) uracil reductase (HTP reductase) Rv1409, (MTCY21B4.26), len: 339 aa. Probable ribG (alternate gene name: ribD), bifunctional riboflavin biosynthesis protein, including diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5-amino-6-(5-phosphoribosylamino) uracil reductase, similar to many e.g. RIBD_ECOLI|P25539 riboflavin-specific deaminase from Escherichia coli (367 aa), FASTA scores: E(): 0, (39.8% identity in 364 aa overlap); etc. Contains PS00903 Cytidine and deoxycytidylate deaminases zinc-binding region signature. In the N-terminal section; belongs to the cytidine and deoxycytidylate deaminases family. In the C-terminal section; belongs to the HTP reductase family. P9WPH1 No intermediary metabolism and respiration P71677 P9WPH1 GO:0050661,GO:0008835,GO:0055114,GO:0009231,GO:0008270,GO:0008703 1.1.1.193,3.5.4.26 P9WPH1 NC_000962.3 Mycobrowser_v5 CDS 1587772 1588482 . - 0 Rv1411c lprG Unknown Conserved lipoprotein LprG Rv1411c, (MTCY21B4.28c), len: 236 aa. lprG (alternate gene name: P27), conserved lipoprotein, similar to Mycobacterium tuberculosis hypothetical lipoproteins e.g. Rv1270c|MTCY50.12 (35.1% identity in 245 aa overlap); Rv1368, Rv2945c. Contains N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein lipid attachment site (PS00013). Note that the Rv1410c-Rv1411c operon seems transcribed from two promoters in Mycobacterium bovis BCG (see Bigi et al., 2000). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WK45 No cell wall and cell processes 3MH8,3MH9,3MHA P0A5I8 P9WK45 GO:0005886,GO:0009405 P9WK45 NC_000962.3 Mycobrowser_v5 CDS 1591671 1592153 . + 0 Rv1416 ribH Riboflavin synthase is a bifunctional enzyme complex involved in riboflavin synthesis. Riboflavin synthase catalyzes the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes the condensation of 5-amino-6-(1'-D)-ribityl- amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone- 4-phosphate yielding 6,7-dimethyl-8-lumazine. Probable riboflavin synthase beta chain RibH (6,7-dimethyl-8-ribityllumazine synthase) (DMRL synthase) (lumazine synthase) Rv1416, (MTCY21B4.34), len: 160 aa. Probable ribH, riboflavin synthase beta chain, similar to many e.g. RISB_ECOLI|P25540 Escherichia coli (156 aa), FASTA scores: opt: 330, E(): 1.8e-15, (44.1% identity in 145 aa overlap). Note alternative GTG start possible overlapping the stop codon of Rv1415|MTCY21B4.33. Belongs to the DMRL synthase family. N-terminus extended since first submission (previously 154 aa). P9WHE9 No intermediary metabolism and respiration 1W19,1W29,2C92,2C94,2C97,2C9B,2C9D,2VI5 P66034 P9WHE9 GO:0004746,GO:0009349,GO:0009231 2.5.1.9 P9WHE9 NC_000962.3 Mycobrowser_v5 CDS 1594042 1595982 . + 0 Rv1420 uvrC Involved in nucleotide excision repair. The ABC excision nuclease is a DNA repair enzyme that catalyzes the excision reaction of UV-damaged nucleotide segments producing oligomers having the modified base(S). Attaches to the UVRA-UVRB complex, displacing UVRA, and the damaged DNA strand is nicked on both sides of the damaged site Probable excinuclease ABC (subunit C - nuclease) UvrC Rv1420, (MTCY21B4.38), len: 646 aa. Probable uvrC, excinuclease ABC, subunit C; nuclease (see citations below), similar to many e.g. UVRC_PSEFL|P32966 Pseudomonas fluorescens (607 aa), fasta scores: opt: 738, E(): 8.4e-39, (36.6% identity in 629 aa overlap). Belongs to the UvrC family. P9WFC5 No information pathways P67426 P9WFC5 GO:0009432,GO:0005737,GO:0009381,GO:0009380,GO:0006289,GO:0003677 P9WFC5 NC_000962.3 Mycobrowser_v5 CDS 1597906 1598883 . + 0 Rv1423 whiA Involved in transcriptional mechanism Probable transcriptional regulatory protein WhiA Rv1423, (MTCY21B4.41-MTCY493.31c), len: 325 aa. Putative whiA, transcriptional regulator, probably equivalent to AL035591|SCC54.10 whiA protein from Streptomyces coelicolor (328 aa), FASTA scores: opt: 1505, E(): 0, (70.4% identity in 324 aa overlap). Also some similarity to O06975|YVCL hypothetical protein from Bacillus subtilis (316 aa), FASTA scores: E(): 1.8e-0 8, (25.7% identity in 304 aa overlap). P9WF45 No regulatory proteins P71692 P9WF45 GO:0043937,GO:0045449,GO:0006350,GO:0003677 P9WF45 NC_000962.3 Mycobrowser_v5 CDS 1612342 1612950 . - 0 Rv1435c Rv1435c Unknown Probable conserved proline, glycine, valine-rich secreted protein Rv1435c, (MTCY493.19), len: 202 aa. Probable conserved Pro-, Gly-, Val-rich secreted protein (see citation below) with a N-terminal signal sequence. Similar at C-terminus to AF017099|AF017099_1 Mycobacterium tuberculosis pGB1 (87 aa), FASTA scores: opt: 550, E(): 2.3e-17, (97.7% identity in 86 aa overlap). Shows some similarity to N-terminus of CPN_DROME|Q02910 calphotin. Drosophila melanogaster (865 aa), FASTA scores: opt: 266, E(): 2.5e-05, (37.2% identity in 191 aa overlap). Contains at least five 7 aa imperfect repeats. Also shows similarity to other Mycobacterium tuberculosis proteins e.g. MTCI237.20c (34.7% identity in 193 aa overlap), MTCI65.25c (36.9% identity in 160 aa overlap) and MTCI65.24c (34.2% identity in 196 aa overlap). O06823 No cell wall and cell processes O06823 NC_000962.3 Mycobrowser_v5 CDS 1613307 1614326 . + 0 Rv1436 gap Involved in second phase of glycolysis (first step) [catalytic activity: D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.] Probable glyceraldehyde 3-phosphate dehydrogenase Gap (GAPDH) Rv1436, (MTCY493.18c), len: 339 aa. Probable gap, Glyceraldehyde 3-phosphate dehydrogenase, highly similar to many e.g. G3P_MYCLE|P46713 Mycobacterium leprae (339 aa), FASTA scores: opt: 1933, E():0, (89.1% identity in 339 aa overlap). Contains PS00071 Glyceraldehyde 3-phosphate dehydrogenase active site. Belongs to the glyceraldehyde 3-phosphate dehydrogenase family. P9WN83 No intermediary metabolism and respiration P64178 P9WN83 GO:0005737,GO:0004365,GO:0006096,GO:0055114,GO:0051287 1.2.1.12 P9WN83 NC_000962.3 Mycobrowser_v5 CDS 2894893 2895960 . - 0 Rv2571c Rv2571c Unknown Probable transmembrane alanine and valine and leucine rich protein Rv2571c, (MTCY227.30), len: 355 aa. Probable transmembrane ala-, val-, leu-rich protein, showing some similarity with other membrane proteins e.g. Q99340|YFDA_CORGL hypothetical integral membrane protein from Corynebacterium glutamicum (Brevibacterium flavum) (359 aa), FASTA scores: opt: 338, E(): 2.5e-13, (29.4% identity in 255 aa overlap); Q9RD86|SCF43.02 putative integral membrane protein from Streptomyces coelicolor (379 aa), FASTA scores: opt: 208, E(): 2.1e-05, (26.05% identity in 303 aa overlap); Q9RD81|SCF43.07 putative integral membrane protein from Streptomyces coelicolor (419 aa), FASTA scores: opt: 205, E(): 3.5e-05, (25.15% identity in 362 aa overlap); etc. P9WL89 No cell wall and cell processes P65015 P9WL89 GO:0005886,GO:0016021 P9WL89 NC_000962.3 Mycobrowser_v5 CDS 1625418 1626962 . - 0 Rv1447c zwf2 Involved in pentose phosphate pathway [catalytic activity: D-glucose 6-phosphate + NADP(+) = D-glucono- 1,5-lactone 6-phosphate + NADPH] Probable glucose-6-phosphate 1-dehydrogenase Zwf2 (G6PD) Rv1447c, (MTCY493.07), len: 514 aa. Probable zwf2 (ZWF), Glucose-6-phosphate 1-dehydrogenase, highly similar to many e.g. G6PD_SYNY3|P73411 Synechocystis sp. (509 aa), FASTA scores: opt: 1578, E(): 0, (46.8% identity in 509 aa overlap). Also similar to M. tuberculosis Rv1121, zwf glucose-6-phosphate 1-dehydrogenase. Contains PS00069 Glucose-6-phosphate dehydrogenase active site. Mycobacterium tuberculosis has two genes for ZWF. This one looks like a classical ZWF. Belongs to the glucose-6-phosphate dehydrogenase family. P9WN73 No intermediary metabolism and respiration P0A584 P9WN73 GO:0006006,GO:0004345,GO:0055114 1.1.1.49 P9WN73 NC_000962.3 Mycobrowser_v5 CDS 1628097 1630199 . - 0 Rv1449c tkt This enzyme, together with transaldolase, provides a link between the glycolytic and pentose-phosphate pathways. It catalyzes the reversible transfer of a two-carbon KETOL unit from xylulose 5-phosphate to an aldose receptor [catalytic activity: sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate] Transketolase Tkt (TK) Rv1449c, (MTCY493.05), len: 700 aa. tkt, transketolase. Highly similar to several e.g. TKT_MYCLE|P46708 transketolase (tk) from Mycobacterium leprae (699 aa), FASTA scores: opt: 4216, E(): 0, (89.1% identity in 700 aa overlap). Start site chosen by homology. Contains PS00801 Transketolase signature 1. Belongs to the transketolase family. Thought to be differentially expressed within host cells (see Triccas et al., 1999). P9WG25 No intermediary metabolism and respiration O06811 P9WG25 GO:0046872,GO:0004802,GO:0008152 2.2.1.1 P9WG25 NC_000962.3 Mycobrowser_v5 CDS 1636004 1638229 . - 0 Rv1452c PE_PGRS28 Function unknown PE-PGRS family protein PE_PGRS28 Rv1452c, (MTCY493.02), len: 741 aa. PE_PGRS28, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), similar to Y03A_MYCTU|Q10637 hypothetical glycine-rich 49.6 kDa protein (603 aa), fasta scores: opt: 2090, E(): 0, (56.3% identity in 641 aa overlap). Q79FP1 No PE/PPE Q79FP1 Q79FP1 Q79FP1 NC_000962.3 Mycobrowser_v5 CDS 1644363 1646138 . - 0 Rv1459c Rv1459c Unknown Possible conserved integral membrane protein Rv1459c, (MTV007.06c), len: 591 aa. Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). Possible conserved integral membrane protein, equivalent to MLCL536.30|Z99125 hypothetical protein from Mycobacterium leprae (593 aa), FASTA scores: opt: 1670, E(): 0, (78.6% identity in 585 aa overlap). Also similar to M. tuberculosis protein Rv2174|MTV021.07 (33.1% identity in 523 aa overlap). O53150 No cell wall and cell processes O53150 NC_000962.3 Mycobrowser_v5 CDS 1646989 1649529 . + 0 Rv1461 Rv1461 Function unknown Conserved protein Rv1461, (MTV007.08), len: 846 aa. Conserved protein. Equivalent of spliced protein from Mycobacterium leprae MLCL536.28c len: 869. Residues 1-253 represent N-extein, and 613-846 the C-extein. The intein present from residues 254 - 612 is different in sequence and site of the insertion from the one present in MLCL536.28c. FASTA scores: Z99125|MLCL536_23 Mycobacterium leprae cosmid L536 (869 aa), opt: 1498 E(): 0, (54.1% identity in 917 aa overlap). The mature protein is similar to Z99120|BSUB0017_150 hypothetical Bacillus subtilis protein (465 aa), FASTA scores: opt:1053, E(): 0, (34.8% identity in 821 aa overlap). The intein shows some similarity to inteins from U67548|MJU67548_6 Methanococcus jannaschii (895 aa), FASTA scores: opt: 181, E(): 0.00023, (25.2% identity in 274 aa overlap). P9WFP7 No conserved hypotheticals P67125 P9WFP7 GO:0016539,GO:0006314,GO:0016226,GO:0005515,GO:0004519 3.1.-.- P9WFP7 NC_000962.3 Mycobrowser_v5 CDS 1651518 1652771 . + 0 Rv1464 csd Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L-selenocystine to produce L-alanine Probable cysteine desulfurase Csd Rv1464, (MTV007.11), len: 417 aa. Probable csd, cysteine desulfurase. Equivalent to Q49690|MLCL536.25C cysteine desulfurase from Mycobacterium leprae (418 aa), FASTA scores: opt: 2333, E(): 0, (85.4% identity in 417 aa overlap); and similar to cysteine desulfurase from other organisms. Also similar to M. tuberculosis proteins Rv3025c|ISCS and Rv3778c. Contains PS00595 Aminotransferases class-V pyridoxal-phosphate attachment site. Belongs to class-V of pyridoxal-phosphate-dependent aminotransferases. CSD subfamily. P9WQ69 No intermediary metabolism and respiration P63516 P9WQ69 GO:0006534,GO:0030170,GO:0031071 2.8.1.7 P9WQ69 NC_000962.3 Mycobrowser_v5 CDS 1653673 1655502 . - 0 Rv1467c fadE15 Function unknown, but involvement in lipid degradation. Probable acyl-CoA dehydrogenase FadE15 Rv1467c, (MTV007.14c), len: 609 aa. Probable fadE15, acyl-CoA dehydrogenase, highly similar to NP_302639.1|NC_002677 acyl-CoA dehydrogenase from Mycobacterium leprae (611 aa). Also highly similar to many e.g. T36481 probable acyl-CoA dehydrogenase (fragment) from Streptomyces coelicolor (491 aa) (has its N-terminus very shorter); NP_384640.1|NC_003047 putative acyl-CoA dehydrogenase protein from Sinorhizobium meliloti (598 aa); ACDS_MEGEL|Q06319 acyl-CoA dehydrogenase (short-chain specific) from Megasphaera elsdenii (383 aa), FASTA scores: E(): 2e-12, (25.4% identity in 410 aa overlap); etc. Also highly similar to fadE5|Rv0244c|MTV034.10c acyl-CoA dehydrogenase from Mycobacterium tuberculosis (611 aa); and similar to other proteins from Mycobacterium tuberculosis. O53158 No lipid metabolism O53158 O53158 GO:0003995,GO:0055114,GO:0050660 1.3.99.- O53158 NC_000962.3 Mycobrowser_v5 CDS 1679322 1680629 . + 0 Rv1490 Rv1490 Unknown Probable membrane protein Rv1490, (MTCY277.12), len: 435 aa. Probable membrane protein. P9WLX1 No cell wall and cell processes P9WLX1 GO:0005886,GO:0016021 P9WLX1 NC_000962.3 Mycobrowser_v5 CDS 1656963 1658936 . + 0 Rv1469 ctpD Cation-transporting ATPase; possibly catalyzes the transport of a cation (possibly cadmium) with the hydrolyse of ATP [catalytic activity: ATP + H(2)O + cation(in) = ADP + phosphate + cation(out)]. Probable cation transporter P-type ATPase D CtpD Rv1469, (MTV007.16), len: 657 aa. Probable ctpD, cation-transporting P-type ATPase D (transmembrane protein), highly similar to others e.g. T35947 probable cation-transporting ATPase from Streptomyces coelicolor (638 aa); NP_442633.1|NC_000911 cation-transporting ATPase (E1-E2 ATPase) from Synechocystis sp. strain PCC 6803 (642 aa), FASTA scores: opt: 1438, E(): 0, (41.9% identity in 592 aa overlap); NP_389268.1|NC_000964 protein similar to heavy metal-transporting ATPase from Bacillus subtilis (637 aa); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. Rv3743c|MTV025.091c|CTPJ (660 aa). Contains PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases), subfamily IB. P9WPT3 No cell wall and cell processes P63685 P9WPT3 GO:0006754,GO:0015662,GO:0005261,GO:0016021,GO:0046872,GO:0030001,GO:0005886,GO:0005524 3.6.3.- P9WPT3 NC_000962.3 Mycobrowser_v5 CDS 1659370 1659741 . + 0 Rv1471 trxB1 Thioredoxin participates in various redox reactions through the reversible oxidation of its active center dithiol, to a disulfide, & catalyzes dithiol-disulfide exchange reactions. Probable thioredoxin TrxB1 Rv1471, (MTV007.18), len: 123 aa. Probable trxB1, thioredoxin, similar to many bacterial thioredoxins e.g. P33636|THI2_ECOLI from Escherichia coli (139 aa), FASTA scores: opt: 290, E(): 1.8e-13, (44.3% identity in 97 aa overlap); etc. Highly similar to Rv1470|TrxA probable thioredoxin from Mycobacterium tuberculosis (124 aa), FASTA scores: opt: 402, E(): 1.2e-32, (54.4% identity in 114 aa overlap). Contains PS00194 Thioredoxin family active site. Belongs to the thioredoxin family. Note that previously known as trxB. L7N664 No intermediary metabolism and respiration Q7D8E1 L7N664 GO:0009055,GO:0006662,GO:0055114,GO:0015035,GO:0045454 1.-.-.- L7N664 NC_000962.3 Mycobrowser_v5 CDS 1659763 1660620 . + 0 Rv1472 echA12 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Possible enoyl-CoA hydratase EchA12 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv1472, (MTV007.19), len: 285 aa. Possible echA12, enoyl-CoA hydratase, highly similar to P53526|ECHH_MYCLE|NP_301896.1|NC_002677 possible enoyl-CoA hydratase/isomerase from Mycobacterium leprae (294 aa), FASTA scores: opt: 1265, E(): 0, (72.0% identity in 271 aa overlap). Also similar to others e.g. CAA66096.1|X97452 enoyl-CoA isomerase from Escherichia coli strain K12 (262 aa); CAC44593.1|AL596162 putative enoyl-CoA hydratase from Streptomyces coelicolor (275 aa); etc. Also similar to others from Mycobacterium tuberculosis e.g. ECHA16|Rv2831|MTCY16B7.11c (249 aa), FASTA scores: opt: 232, E(): 1.3e-15, (33.8% identity in 204 aa overlap); etc. P9WNN7 No lipid metabolism O53163 P9WNN7 GO:0006631,GO:0004300 4.2.1.17 P9WNN7 NC_000962.3 Mycobrowser_v5 CDS 1662641 1663204 . - 0 Rv1474c Rv1474c Involved in transcriptional mechanism Probable transcriptional regulatory protein Rv1474c, (MTV007.21c), len: 187 aa. Probable transcription regulator, equivalent to AF0021|AF002133_1 transcriptional regulator from Mycobacterium avium strain GIR10 (82 aa), FASTA scores: opt: 490, E(): 6.7e-26, (92.5% identity in 80 aa overlap). Also similar to Q59431|UIDR_ECOLI UID operon repressor (GUS operon) from Escherichia coli (196 aa), FASTA scores: opt: 192, E(): 5.8e-06, (28.5% identity in 172 aa overlap). Belongs to the TetR/AcrR family of transcriptional regulators. Helix turn helix motif predicted at aa 33-54 (+3.40 SD). O53165 No regulatory proteins O53165 O53165 GO:0006350,GO:0003700,GO:0006355 O53165 NC_000962.3 Mycobrowser_v5 CDS 1666990 1668408 . + 0 Rv1477 ripA Unknown, but supposed involvement in virulence. Peptidoglycan hydrolase Rv1477, (MTV007.24), len: 472 aa. RipA, peptidoglycan hydrolase (see Hett et al., 2007). Secreted, cell-associated protein. The last 277 residues are nearly identical to those of AF0060|AF006054_1 hypothetical invasion protein INV1 from Mycobacterium tuberculosis (277 aa), FASTA scores: opt: 1833, E(): 0, (98.2% identity in 277 aa overlap); also very similar to AF0021|AF002133_4 invasin 1 protein from Mycobacterium avium (273 aa), FASTA scores: opt: 1452, E(): 0, (78.1% identity in 279 aa overlap). Similar to Rv1566c|MTCY336.37|Z95586 Mycobacterium tuberculosis cosmid (230 aa), FASTA scores: opt: 528, E(): 4.4e-20, (52.0% identity in 150 aa overlap); and weakly similar to p60 proteins of Listeria spp throughout its length e.g. M80351|LISIAPB_1 Listeria monocytogenes iap-related protein (478 aa), FASTA scores: opt: 251, E(): 8e-06, (24.4% identity in 487 aa overlap). C-terminal domain highly similar to next orf Rv1478|MTV007.25. Interacts with RpfB and RpfE (see Hett et al., 2007). Predicted to be an outer membrane protein (See Song et al., 2008). O53168 No virulence, detoxification, adaptation 3PBC,3S0Q,2XIV,3NE0 O53168 O53168 O53168 NC_000962.3 Mycobrowser_v5 CDS 1678552 1678908 . + 0 Rv1489 Rv1489 Function unknown Conserved protein Rv1489, len: 118 aa. Conserved protein, similar to hypothetical proteins from Mycobacterium avium subsp. paratuberculosis and Streptomyces coelicolor e.g. AJ250017_1 insertion sequence IS900, Locus 3, putative invasion protein from M. paratuberculosis (138 aa), FASTA scores: opt: 120, E(): 0.26, (34.375% identity in 96 aa overlap); SCD6.11c|AL353815_11 possible integral membrane protein from Streptomyces coelicolor (136 aa), FASTA scores: opt: 106, E(): 2.2, (35.9% identity in 103 aa overlap). ORF predicted by GC plot. Replaces previous Rv1489c on other strand. L7N692 No conserved hypotheticals L7N692 NC_000962.3 Mycobrowser_v5 CDS 1672457 1673299 . - 0 Rv1482c Rv1482c Function unknown Conserved hypothetical protein Rv1482c, (MTCY277.03c), len: 280 aa. Conserved hypothetical protein, highly similar to O07396|AF002133 Mycobacterium avium protein MAV346 (346 aa), FASTA scores: E(): 0, (65.2% identity in 342 aa overlap); slight similarity to GRPE_ECOLI|P09372 heat shock protein from E. coli (197 aa), FASTA scores: opt: 139, E(): 0.012, (28.3% identity in 159 aa overlap). Similar to Mycobacterium tuberculosis hypothetical proteins Rv3517, Rv3555c, Rv3714c, Rv1073, etc. Start changed since first submission (-59 aa). P71763 No conserved hypotheticals P71763 P71763 NC_000962.3 Mycobrowser_v5 CDS 1674202 1675011 . + 0 Rv1484 inhA This isozyme is involved in mycolic acid biosynthesis. Second reductive step in fatty acid biosynthesis. Involved in the resistance against the antituberculosis drugs isoniazid and ethionamide [catalytic activity: acyl-[acyl-carrier protein] + NAD(+) = trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH]. NADH-dependent enoyl-[acyl-carrier-protein] reductase InhA (NADH-dependent enoyl-ACP reductase) Rv1484, (MTCY277.05), len: 269 aa. InhA, NADH-dependent enoyl-[acyl-carrier-protein] reductase (see citations below). Identical to INHA_MYCTU|P46533 enoyl-[acyl-carrier-protein] reductase from Mycobacterium tuberculosis and G1155270 Mycobacterium bovis enoyl acp reductase. Some similarity to the short-chain dehydrogenases/reductases (SDR) family. P9WGR1 No lipid metabolism 1BVR,1ENY,1ENZ,1P44,1P45,1ZID,2AQ8,2AQH,2AQI,2AQK,2B35,2B36,2B37,2H7I,2H7L,2H7M,2H7N,2H7P,2H9I,2IDZ,2IE0,2IEB,2IED,2NSD,2NTJ,2NV6,2PR2,2X22,2X23,3FNE,3FNF,3FNG,3FNH,3OEW,3OEY,3OF2 P9WGR1 GO:0004318,GO:0006633,GO:0055114,GO:0046677 1.3.1.9 ETH,INH P9WGR1 NC_000962.3 Mycobrowser_v5 CDS 1677397 1678542 . + 0 Rv1488 Rv1488 Function unknown Possible exported conserved protein Rv1488, (MTCY277.09), len: 381 aa. Possible exported conserved protein; contains possible N-terminal signal sequence. Similar to YBBK_ECOLI|P77367 hypothetical protein ybbK from Escherichia coli (305 aa), FASTA scores: opt: 716, E(): 0, (37.1% identity in 307 aa overlap). Similar to stomatin-like proteins e.g. AF065260|AF065260_1 Clostridium difficile (320 aa), FASTA scores: opt: 767, E(): 0, (42.3% identity in 307 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). P9WPR9 No cell wall and cell processes P63693 P9WPR9 GO:0016021 P9WPR9 NC_000962.3 Mycobrowser_v5 CDS 1681208 1681966 . - 0 Rv1491c Rv1491c Unknown Conserved membrane protein Rv1491c, (MTCY277.13c), len: 252 aa. Conserved membrane protein. Similar to hypothetical proteins from many organisms e.g. YDJZ_ECOLI|P76221 Escherichia coli (235 aa), FASTA scores: opt: 223, E():6.7 e-07, (31.7% identity in 145 aa overlap); AL133252|SCE46.15 Streptomyces coelicolor (249 aa), FASTA scores: opt: 378, E(): 1.5e-17, (39.1% identity in 169 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical protein Rv0625c. P9WFS3 No cell wall and cell processes P67117 P9WFS3 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1682157 1684004 . + 0 Rv1492 mutA Involved in propionic acid fermentation. Catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates [catalytic activity: (R)-2-methyl-3-oxopropanoyl-CoA = succinyl- CoA] Probable methylmalonyl-CoA mutase small subunit MutA (MCM) Rv1492, (MTCY277.14), len: 615 aa. Probable mutA, Methylmalonyl-CoA mutase small-subunit, strong similarity to e.g. MUTA_STRCM|Q05064 methylmalonyl-CoA mutase beta-subunit from Streptomyces cinnamonensis (616 aa), FASTA scores: opt: 1512, E(): 0, (45.9% identity in 628 aa overlap). Contains PS00213 Lipocalin signature, PS00544 Methylmalonyl-CoA mutase signature. Belongs to the methylmalonyl-CoA mutase family. P9WJK7 No lipid metabolism P65485 P9WJK7 GO:0019652,GO:0004494,GO:0031419 5.4.99.2 P9WJK7 NC_000962.3 Mycobrowser_v5 CDS 1690407 1690805 . + 0 Rv1499 Rv1499 Unknown Hypothetical protein Rv1499, (MTCY277.21), len: 132 aa. Hypothetical unknown protein; was initially longer but has been shortened (-24 aa) owing to overlap with Rv1498A. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P71780 No conserved hypotheticals P71780 NC_000962.3 Mycobrowser_v5 CDS 1690850 1691878 . + 0 Rv1500 Rv1500 Function unknown Probable glycosyltransferase Rv1500, (MTCY277.22), len: 342 aa. Probable glycosyltransferase, hydrophobic domain near C-terminus. Some similarity to putative glycosyl-transferases from Bacillus subtilis e.g. O34319|YKCC_BACSU (323 aa), opt: 490, E(): 6.1e-25, (28.85% identity in 312 aa overlap) and to N-acetyl glucosamine transferases. Also similar to G1001347 hypothetical 36.7 kDa protein (318 aa), FASTA scores: opt: 523, E(): 7.2e-26, (30.6% identity in 307 aa overlap). P9WMX5 No intermediary metabolism and respiration P71781 P9WMX5 GO:0005886,GO:0016757,GO:0016021 2.4.-.- P9WMX5 NC_000962.3 Mycobrowser_v5 CDS 1703074 1704096 . + 0 Rv1511 gmdA Unknown, probably involved in nucleotide-sugar metabolism GDP-D-mannose dehydratase GmdA (GDP-mannose 4,6 dehydratase) (GMD) Rv1511, (MTCY277.33), len: 340 aa. Probable gmdA, GDP-D-mannose dehydratase, equivalent to AF125999|AF125999_13 Mycobacterium avium enzyme (343 aa), FASTA scores: opt: 2085, E(): 0, (89.1% identity in 338 aa overlap); similar to G755218 pseudomonas aeruginosa GDP-D-mannose dehydratase (GCA) (323 aa), FASTA scores: opt: 1073, E(): 0, (51.9% identity in 320 aa overlap); and to S74433 GDP-D-mannose dehydratase rfbD - Syn (362 aa), FASTA scores: opt: 1405, E(): 0, (63.9% identity in 327 aa overlap). P71790 No intermediary metabolism and respiration P71790 P71790 GO:0019673,GO:0050662,GO:0005622,GO:0008446 4.2.1.47 P71790 NC_000962.3 Mycobrowser_v5 CDS 1704093 1705061 . + 0 Rv1512 epiA Unknown, probably involved in nucleotide-sugar metabolism Probable nucleotide-sugar epimerase EpiA Rv1512, (MTCY277.34), len: 322 aa. Probable epiA, nucleotide sugar epimerase, equivalent to AJ223832|MAS223832_4 from Mycobacterium avium silvaticum (339 aa), FASTA scores: opt: 1821, E(): 0, (84.6% identity in 318 aa overlap); and similar to WCAG_ECOLI|P32055 colanic acid biosynthesis protein wcaG (321 aa), FASTA scores: opt: 835, E(): 0, (53.5% identity in 316 aa overlap). P71791 No intermediary metabolism and respiration P71791 P71791 GO:0044237,GO:0050662,GO:0003824 P71791 NC_000962.3 Mycobrowser_v5 CDS 1712302 1714053 . + 0 Rv1521 fadD25 Function unknown, but involvement in lipid degradation. Probable fatty-acid-AMP ligase FadD25 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) Rv1521, (MTCY19G5.07), len: 583 aa. Probable fadD25, fatty-acid-AMP synthetase, highly similar to many e.g. P71495|U75685 acyl-CoA synthase from Mycobacterium bovis (582 aa), FASTA scores: opt: 2486, E(): 0, (63.4% identity in 584 aa overlap); NP_301232.1|NC_002677 acyl-CoA synthetase from Mycobacterium leprae (579 aa); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. fadD24 (584 aa); fadD28 (580 aa); etc. P9WQ45 No lipid metabolism Q50586 P9WQ45 GO:0016021,GO:0016874,GO:0005886,GO:0006631 6.2.1.- P9WQ45 NC_000962.3 Mycobrowser_v5 CDS 1714172 1717612 . - 0 Rv1522c mmpL12 Unknown. Thought to be involved in fatty acid transport. Probable conserved transmembrane transport protein MmpL12 Rv1522c, (MTCY19G5.06), len: 1146 aa. Probable mmpL12, conserved transmembrane transport protein (see Tekaia et al., 1999), member of RND superfamily. Strong similarity to many Mycobacterial membrane proteins e.g. Q49619|G466786 putative transport protein B1170_C1_181 from Mycobacterium leprae (1008 aa), FASTA scores: opt: 2418, E(): 0, (51.0% identity in 1006 aa overlap); etc. Also highly similar to MmpL8|MTCY48.08c|Rv3823c probable conserved transmembrane transport protein from Mycobacterium tuberculosis, FASTA score: (34.3% identity in 376 aa overlap); and some similarity to MmpL10|MTCY20G9|Rv1183 probable conserved transmembrane transport protein, FASTA score: (27.2% identity in 1011 aa overlap). Belongs to the MmpL family. P9WJT7 No cell wall and cell processes Q50585 P9WJT7 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1720780 1722060 . - 0 Rv1526c Rv1526c Unknown; thought to be involved in cellular metabolism. Probable glycosyltransferase Rv1526c, (MTCY19G5.02), len: 426 aa. Probable glycosyltransferase, highly similar to G467196 Protein L518_C2_147 from Mycobacterium leprae (421 aa), FASTA scores, opt: 1497, E(): 0, (55.0% identity in 424 aa overlap); similar to G452504 rhamnosyltransferase (24.7% identity in 433 aa overlap); and P96565|U84350 glycosyltransferase GTFE from Amycolatopsis orientalis (408 aa), E(): 3.4e-24, (28.4% identity in 429 aa overlap), also high similarity to Rv1524|MTCY19G5.04c (58.7 % identity in 416 aa overlap). P9WLV1 No intermediary metabolism and respiration P64869 P9WLV1 GO:0030259,GO:0016758,GO:0005975 P9WLV1 NC_000962.3 Mycobrowser_v5 CDS 1753716 1755431 . + 0 Rv1550 fadD11 Function unknown, but involvement in lipid degradation. Probable fatty-acid-CoA ligase FadD11 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv1550, (MTCY48.15c), len: 571 aa. Probable fadD11, fatty-acid-CoA synthetase, similar, except in N-terminus, to many e.g. SC6A5.39|T35430 probable long-chain-fatty-acid--CoA ligase from Streptomyces coelicolor (612 aa); NP_301672.1|NC_002677 putative long-chain-fatty-acid-CoA ligase from Mycobacterium leprae (600 aa); P44446|LCFH_HAEIN putative long-chain-fatty-acid-CoA ligase from Haemophilus influenzae (607 aa), FASTA scores: opt: 762, E(): 2.3e-38, (34.4% identity in 436 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family. Possible frameshift with respect to previous ORF Rv1549|MTCY48.16c but we can find no sequence error to account for this. P9WQ53 No lipid metabolism Q10776 P9WQ53 GO:0016021,GO:0016874,GO:0005886,GO:0006631 6.2.1.- P9WQ53 NC_000962.3 Mycobrowser_v5 CDS 1739856 1741247 . + 0 Rv1537 dinX Involved in DNA metabolism [catalytic activity: N deoxynucleoside triphosphate = N diphosphate + {DNA}N]. Probable DNA polymerase IV DinX (pol IV 1) (DNA nucleotidyltransferase (DNA-directed)) Rv1537, (MTCY48.28c, MT1589), len: 463 aa. Probable dinX (alternate gene name: dinB1), DNA polymerase IV. Similar to umuC, mucB, samb, and impb (UV protection and mutation) e.g. IMPB_SALTY|P18642 impb protein from Salmonella typhimurium (424 aa), FASTA scores: opt: 386, E(): 1.7e-17, (27.5% identity in 415 aa overlap); etc. Also similar to Mycobacterium tuberculosis Rv3056|dinP. Belongs to the DNA polymerase type-Y family. P9WNT3 No information pathways P63985 P9WNT3 GO:0006261,GO:0003887,GO:0005737,GO:0003684,GO:0000287,GO:0006281 2.7.7.7 P9WNT3 NC_000962.3 Mycobrowser_v5 CDS 1751297 1753333 . - 0 Rv1548c PPE21 Function unknown PPE family protein PPE21 Rv1548c, (MTCY48.17), len: 678 aa. PPE21, Member of the Mycobacterium tuberculosis PPE family, similar to several e.g. YHS6_MYCTU|P42611 hypothetical 50.6 kDa protein in hsp65 3' region (517 aa), FASTA scores: opt:1142, E(): 0, (40.6% identity in 616 aa overlap); also similar to MTCY31.06c (54.9% identity in 381 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). P9WI21 No PE/PPE Q10778 P9WI21 GO:0005886,GO:0016021 P9WI21 NC_000962.3 Mycobrowser_v5 CDS 1757681 1759432 . + 0 Rv1552 frdA Involved in interconversion of fumarate and succinate (anaerobic respiration) [catalytic activity: succinate + acceptor = fumarate + reduced acceptor]. Probable fumarate reductase [flavoprotein subunit] FrdA (fumarate dehydrogenase) (fumaric hydrogenase) Rv1552, (MTCY48.13c), len: 583 aa. Probable frdA, fumarate reductase, flavoprotein subunit, highly similar to others e.g. P00363|FRDA_ECOLI fumarate reductase flavoprotein subunit from Escherichia coli strain K12 (601 aa), FASTA scores: opt: 2102, E(): 0, (54.7% identity in 585 aa overlap); NP_232284.1|NC_002505 fumarate reductase, flavoprotein subunit from Vibrio cholerae (602 aa); frdA|NP_438995.1|NC_000907 fumarate reductase, flavoprotein subunit from Haemophilus influenzae (599 aa); etc. Contains PS00504 Fumarate reductase / succinate dehydrogenase FAD-binding site. Note that fumarate reductase forms part of an enzyme complex containing four subunits: a flavoprotein (Rv1552|frdA), an iron-sulfur (Rv1553|frdB), and two hydrophobic anchor proteins (Rv1554|frdC and Rv1555|frdD). P9WN91 No intermediary metabolism and respiration P64174 P9WN91 GO:0009061,GO:0009055,GO:0022900,GO:0000104,GO:0006810,GO:0050660 1.3.99.1 P9WN91 NC_000962.3 Mycobrowser_v5 CDS 1761744 1762937 . + 0 Rv1557 mmpL6 Unknown. Thought to be involved in fatty acid transport. Probable conserved transmembrane transport protein MmpL6 Rv1557, (MTCY48.08c), len: 397 aa. Probable mmpL6, conserved transmembrane transport protein (see citations below). Member of RND superfamily, with strong similarity to C-terminal part of members of large Mycobacterial membrane protein family belonging to RND superfamily including: mmpL1, mmpL2, mmpL3, etc. Probably truncated (see Brosch et al., 2002). Belongs to the MmpL family. P9WJU9 No cell wall and cell processes Q10773 P9WJU9 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1765400 1767142 . - 0 Rv1562c treZ Involved in trehalose biosynthesis (protective effect). Mycobacteria can produce trehalose from glucose 6-phosphate and UDP-glucose (the OTSA-OTSB pathway) from glycogen-like alpha(1-->4)-linked glucose polymers (the trey-TREZ pathway) and from maltose (the TRES pathway). Seems to have additional alpha-glucosidase activity. Maltooligosyltrehalose trehalohydrolase TreZ Rv1562c, (MTCY48.03), len: 580 aa. TreZ (previously called glgZ), Maltooligosyltrehalose trehalohydrolase, confirmed biochemically (see citation below). Similar to Q44316|D63343 TREZ maltooligosyl trehalose trehalohydrolase from arthrobacter SP (598 aa), FASTA scores: opt: 2071, E(): 0, (52.2% identity in 582 aa overlap); also similar to 1,4-alpha-glucan branching enzymes e.g. GLGB_BACST|P30538 (639 aa), FASTA scores: opt: 313, E(): 3.8e-13, (27.5% identity in 462 aa overlap). Also similar to Mycobacterium tuberculosis proteins Rv1326c|glgB, and Rv1563c treY (previously glgY). P9WQ23 No virulence, detoxification, adaptation Q10769 P9WQ23 GO:0043169,GO:0005737,GO:0005992,GO:0033942 3.2.1.141 P9WQ23 NC_000962.3 Mycobrowser_v5 CDS 1769436 1771601 . - 0 Rv1564c treX Possibly involved in trehalose biosynthesis (protective effect). Mycobacteria can produce trehalose from glucose 6-phosphate and UDP-glucose (the OtsA-OtsB pathway) from glycogen-like alpha(1-->4)-linked glucose polymers (the TreY-TreZ pathway) and from maltose (the TreS pathway). Probable maltooligosyltrehalose synthase TreX Rv1564c, (MTCY48.01), len: 721 aa. Probable treX (previously called glgX), Maltooligosyltrehalose synthase. Strong similarity to D83245|g1890053 treX, glycogen debranching enzyme (glgX) from Sulfolobus acidocaldarius (713 aa), FASTA score: opt: 2396, E(): 0, (48.4% identity in 709 aa overlap); similar to GLGX_HAEIN|P45178 glycogen operon protein glgx (659 aa), FASTA scores: opt: 1512, E(): 0, (42.3% identity in 645 aa overlap). P9WQ25 No virulence, detoxification, adaptation P0A4Y4 P9WQ25 GO:0005980,GO:0004133,GO:0004553,GO:0043169 3.2.1.- P9WQ25 NC_000962.3 Mycobrowser_v5 CDS 1771640 1773829 . - 0 Rv1565c Rv1565c Unknown Conserved hypothetical membrane protein Rv1565c, (MTCY336.38), len: 729 aa. Conserved hypothetical membrane protein, some similarity to O05402 hypothetical 72.2 kDa protein from Bacillus subtilis (634 aa), FASTA results: opt: 384, E(): 4.8e-17, (29.1% identity in 378 aa overlap); and to Y392_HAEIN|P43993 hypothetical protein hi0392 from H. influenzae (245 aa), FASTA results: opt: 265, E(): 5.5e-10, (28.3% identity in 247 aa overlap). C-terminal half equivalent to AL049478|MLCL458_19 (274 aa) (78.5% identity in 274 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins Rv0111, Rv0228, Rv1254, Rv0517. N-terminal half hydrophobic. O06625 No cell wall and cell processes O06625 O06625 GO:0016747,GO:0016020 NC_000962.3 Mycobrowser_v5 CDS 1775392 1776705 . + 0 Rv1568 bioA Involved in bioconversion of pimelate into dethiobiotin [catalytic activity: S-adenosyl-L-methionine + 8-amino-7- oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8- diaminononanoate]. Supposedly involved in stationary-phase survival. Adenosylmethionine-8-amino-7-oxononanoate aminotransferase BioA Rv1568, (MTCY336.35c), len: 437 aa. bioA, adenosylmethionine-8-amino-7-oxononanoate aminotransferase , equivalent to a predicted homologous protein from Mycobacterium smegmatis (see citation below). Highly similar to BIOA_MYCLE|P4548 from Mycobacterium leprae (436 aa), FASTA results: opt: 2534, E(): 0, (85.1% identity in 436 aa overlap). Also similar to other Mycobacterium tuberculosis proteins e.g. MTCY227.12c (449 aa), FASTA score: E(): 3.5e-16, (29.5% identity in 421 aa overlap). Contains aminotransferases class-III pyridoxal-phosphate attachment site (PS00600). Belongs to class-III of pyridoxal-phosphate-dependent aminotransferases. P9WQ81 No intermediary metabolism and respiration 3BV0,3LV2,3TFT,3TFU P0A4X6 P9WQ81 GO:0009102,GO:0005737,GO:0030170,GO:0004015 2.6.1.62 P9WQ81 NC_000962.3 Mycobrowser_v5 CDS 1780643 1782064 . - 0 Rv1576c Rv1576c Unknown Probable PhiRv1 phage protein Rv1576c, (MTCY336.28), len: 473 aa. Probable phiRV1 phage protein (capsid subunit) (see citation below). Highly similar to hypothetical Mycobacterium tuberculosis protein Rv2650c|MTCY441.19 phiRV2 phage related protein, FASTA scores: opt: 2782, E(): 0, (89.1% identity in 468 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06614 No insertion seqs and phages O06614 NC_000962.3 Mycobrowser_v5 CDS 1784497 1785912 . - 0 Rv1582c Rv1582c Unknown Probable PhiRv1 phage protein Rv1582c, (MTCY336.22), len: 471 aa. Probable phiRv1 phage protein (see citation below). N-terminus is similar to C-terminus of Q38030 ORF9 Bacteriophage phi-C31 (519 aa), FASTA scores: opt: 331, E(): 6.5e-15, (28.5% identity in 235 aa overlap); and C-terminus to whole of Q38031 ORF10 of Bacteriophage phi-C31 (202 aa), FASTA scores: opt: 353, E(): 1e-16, (31.1% identity in 190 aa overlap). Also similar to part of AB016282|AB016282_42 Bacteriophage phi-105 (806 aa), FASTA scores: opt: 790, E(): 0, (32.7% identity in 459 aa overlap). Similarity to other phage proteins described as putative DNA-polymerase or DNA-primase. Also slightly similar to MTCY441.24c, FASTA scores: E(): 0.0055, (36.0% identity in 75 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06608 No insertion seqs and phages O06608 O06608 O06608 NC_000962.3 Mycobrowser_v5 CDS 1787096 1788505 . - 0 Rv1586c Rv1586c Integration of phiRv1 into chromosome. Probable PhiRv1 integrase Rv1586c, (MTCY336.18), len: 469 aa. Probable phiRv1 integrase, possibly member of the serine family of recombinases (see citation below), similar to several bacteriophage integrases e.g. Q37839 ORF469 protein from Bacteriophage R4 (469 aa), FASTA scores: opt: 623, E(): 1.6e-29, (31.1% identity in 482 aa overlap); and Bacteriophage TP901-1. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06604 No insertion seqs and phages O06604 O06604 GO:0006310,GO:0000150,GO:0003677 O06604 NC_000962.3 Mycobrowser_v5 CDS 1802035 1802667 . + 0 Rv1601 hisB Histidine biosynthesis (seventh step) [catalytic activity: D-erythro-1-(imidazol-4-YL)glycerol 3- phosphate = 3-(imidazol-4-YL)-2-oxopropyl phosphate + H(2)O] Probable imidazole glycerol-phosphate dehydratase HisB Rv1601, (MTCY336.03c), len: 210 aa. Probable hisB, imidazole glycerol-phosphate dehydratase. Similar to many e.g. HIS7_STRCO|P16247 from Streptomyces coelicolor (197 aa),FASTA results: opt: 763, E(): 0, (57.4% identity in 202 aa overlap). Belongs to the imidazoleglycerol-phosphate dehydratase family. P9WML9 No intermediary metabolism and respiration P64368 P9WML9 GO:0000105,GO:0004424,GO:0005737 4.2.1.19 P9WML9 NC_000962.3 Mycobrowser_v5 CDS 1793997 1794707 . - 0 Rv1593c Rv1593c Function unknown Conserved protein Rv1593c, (MTCY336.11), len: 236 aa. Conserved protein, highly similar to Q49628|B1170_F1_44 from Mycobacterium leprae (286 aa), FASTA scores: opt: 1304, E (): 0, (85.4% identity in 233 aa overlap); similar to several putative DNA hydrolases e.g. Q9S233|SCI51.07C from Streptomyces coelicolor (239 aa), FASTA scores: opt: 415, E(): 4.6e-20, (34.8% identity in 221 aa overlap); also similar to P74291|SLR1690 hypothetical protein from synechocystis (261 aa), FASTA scores: opt: 228, E(): 1.4e-17, (31.5% identity in 213 aa overlap). O06597 No conserved hypotheticals O06597 O06597 GO:0016787 O06597 NC_000962.3 Mycobrowser_v5 CDS 1795805 1797388 . + 0 Rv1595 nadB Quinolinate biosynthesis. Catalyzes the oxidation of L-aspartate to iminoaspartate which is condensed with dihydroxyacetone phosphate to quinolinate under the action of quinolinate synthase a [catalytic activity: L-aspartate + H(2)O + O(2) = oxaloacetate + NH(3) + H(2)O(2)] Probable L-aspartate oxidase NadB Rv1595, (MTCY336.09c), len: 527 aa. Probable nadB, L-aspartate oxidase. Similar to many e.g. Q49617 L-aspartate oxidase (quinolinate synthetase) from Mycobacterium leprae (424 aa), FASTA results: opt: 2152, E(): 0, (82.0% identity in 400 aa overlap). Also shows some similarity to Rv1552 frdA from Mycobacterium tuberculosis (583 aa), FASTA results: E(): 1e-10, (35.3% identity in 566 aa overlap). Heterodimer. The quinolinate synthetase complex consists of the two enzymes quinolinate synthetase a and B. P9WJJ9 No intermediary metabolism and respiration P65499 P9WJJ9 GO:0009435,GO:0005737,GO:0009055,GO:0055114,GO:0008734 1.4.3.16 P9WJJ9 NC_000962.3 Mycobrowser_v5 CDS 1947861 1949345 . + 0 Rv1722 Rv1722 Function unknown, but supposed involvement in lipid metabolism Possible carboxylase Rv1722, (MTCY04C12.07), len: 494 aa. Possible carboxylases. Weak similarity to several e.g. ACCC_BACSU|P49787 biotin carboxylase from Bacillus subtilis (448 aa), fasta scores: opt: 171, E(): 0.00021, (22.8% identity in 237 aa overlap). P71980 No lipid metabolism P71980 GO:0005524,GO:0003824 P71980 NC_000962.3 Mycobrowser_v5 CDS 1800896 1802038 . + 0 Rv1600 hisC1 Histidine biosynthesis (eighth step) [catalytic activity: L-histidinol-phosphate + 2-oxoglutarate = 3-(imidazol-4-YL)-2-oxopropyl phosphate + L-glutamate] Probable histidinol-phosphate aminotransferase HisC1 Rv1600, (MTCY336.04c), len: 380 aa. Probable hisC1, histidinol-phosphate aminotransferase O06591. Similar to many e.g. HIS8_STRCO|P16246 from Streptomyces coelicolor (369 aa), FASTA results: opt: 1353, E(): 0, (59.0% identity in 356 aa overlap). Some similarity to other Mycobacterium tuberculosis aminotransferases e.g. Rv3772|MTCY13D12.06, FASTA results: E(): 7.4e-25, (33.7% identity in 365 aa overlap). Contains aminotransferases class-II pyridoxal-phosphate attachment site (PS00599). Belongs to class-II of pyridoxal-phosphate-dependent aminotransferases. Note that previously known as hisC. P9WML7 No intermediary metabolism and respiration P0A678 P9WML7 GO:0004400,GO:0030170,GO:0000105 2.6.1.9 P9WML7 NC_000962.3 Mycobrowser_v5 CDS 1892270 1893562 . - 0 Rv1666c cyp139 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Probable cytochrome P450 139 Cyp139 Rv1666c, (MT1706, MTV047.02c), len: 430 aa. Probable cyp139, cytochrome P450, similar to many e.g. U38537|APU38537_7 from Anabaena sp. (459 aa), FASTA scores: opt: 516, E(): 1.7e-26, (25.8% identity in 418 aa overlap). Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. Belongs to the cytochrome P450 family. P9WPM1 No intermediary metabolism and respiration P63719 P9WPM1 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPM1 NC_000962.3 Mycobrowser_v5 CDS 1805653 1806000 . + 0 Rv1606 hisI Involved in histidine biosynthesis pathway (at the third step) [catalytic activity: 5-phosphoribosyl-AMP + H(2)O = 5-(5-phospho-D-ribosylaminoformimino)-1-(5-phospho-ribosyl) imidazole-4- carboxamide.] Probable phosphoribosyl-AMP 1,6 cyclohydrolase HisI Rv1606, (MTV046.04), len: 115 aa. Probable hisI, phosphoribosyl-AMP 1,6 cyclohydrolase, similar to several e.g. X82010|RSHISI_2 HISI from Rhodobacter sphaeroides (119 aa), FASTA scores: opt: 378, E(): 2.8e-21, (52.3% identity in 109 aa overlap); etc. P9WMM7 No intermediary metabolism and respiration P0A5B3 P9WMM7 GO:0000105,GO:0004635,GO:0005737 3.5.4.19 P9WMM7 NC_000962.3 Mycobrowser_v5 CDS 1806181 1807263 . + 0 Rv1607 chaA Involved in transport of ions (presumably calcium) across the membrane. Responsible for the translocation of the substrate across the membrane. Probable ionic transporter integral membrane protein ChaA Rv1607, (MTV046.05), len: 360 aa. Probable chaA, ionic transporter integral membrane protein, putative calcium/proton antiporter, similar to many e.g. P31801|CHAA_ECOLI calcium/proton antiporter from Escherichia coli (366 aa), FASTA scores: opt: 736, E(): 0, (35.9% identity in 351 aa overlap). Equivalent to Mycobacterium leprae AL049913|MLCB1610_21 (77.7% identity in 364 aa overlap). Seems to belong to the CaCA family. O53910 No cell wall and cell processes O53910 O53910 GO:0055085,GO:0016021 O53910 NC_000962.3 Mycobrowser_v5 CDS 1807903 1809453 . + 0 Rv1609 trpE Involved in tryptophan biosynthesis pathway (at the first step). Supposed tetramer of two components I and two components II: component I (Rv1609|trpE) catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II (Rv0013|trpG) provides glutamine amidotransferase activity [catalytic activity: chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate]. Anthranilate synthase component I TrpE (glutamine amidotransferase) Rv1609, (MTCY01B2.01, MTV046.07), len: 516 aa. trpE, anthranilate synthase component I. FASTA best: TRPE_CLOTM|P14953 anthranilate synthase component I from Clostridium thermocellum (494 aa), E(): 0, (42.6% identity in 498 aa overlap). Some similarity to Rv2386c|MTCY253.35, E(): 6.3e-17; and Rv3215|MTCY07D11.11c, E(): 5.7e-15. Belongs to the anthranilate synthase component I family. P9WFX3 No intermediary metabolism and respiration P67001 P9WFX3 GO:0000162,GO:0004049 4.1.3.27 P9WFX3 NC_000962.3 Mycobrowser_v5 CDS 1811127 1812359 . + 0 Rv1612 trpB Tryptophan biosynthesis pathway (fifth last step). The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine. [catalytic activity: L-serine + 1-(indol-3-YL)glycerol 3-phosphate = L-tryptophan + glyceraldehyde 3-phosphate + H(2)O] Tryptophan synthase, beta subunit TrpB Rv1612, (MTCY01B2.04), len: 410 aa. TrpB, tryptophan synthase beta chain. Equivalent to AL049913|MLCB1610_25 from Mycobacterium leprae (340 aa) (88.5% identity in 331 aa overlap). Similar to others e.g. TRPB_CAUCR|P12290 tryptophan synthase beta chain from Caulobacter crescentus (406 aa), FASTA scores: opt: 1662, E(): 0, (60.6% identity in 404 aa overlap). Belongs to the TrpB family. Tetramer of two alpha and two beta chains. P9WFX9 No intermediary metabolism and respiration 2O2E,2O2J P66984 P9WFX9 GO:0000162,GO:0004834,GO:0030170 4.2.1.20 P9WFX9 NC_000962.3 Mycobrowser_v5 CDS 1819963 1821693 . - 0 Rv1620c cydC Involved in active transport across the membrane of component linked with the assembly of cytochrome: involved in cytochrome biogenesis (aerobic respiration). Responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane. Probable 'component linked with the assembly of cytochrome' transport transmembrane ATP-binding protein ABC transporter CydC Rv1620c, (MTCY01B2.12c), len: 576 aa. Probable cydC, transmembrane ATP-binding protein ABC transporter involved in transport of component linked with the assembly of cytochrome (see citation below), similar to others e.g. CYDC_ECOLI|P23886 transport ATP-binding protein from Escherichia coli (573 aa), FASTA scores: opt: 631, E(): 1.6e-30, (28.5% identity in 569 aa overlap); C-terminal part of AL034355|SCD78_14 from Streptomyces coelicolor (1172 aa), FASTA scores: opt: 956, E(): 0, (38.8% identity in 554 aa overlap); etc. Contains (PS00211) ABC transporters family signature, and (PS00017) ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters). O06137 No intermediary metabolism and respiration O06137 O06137 GO:0042626,GO:0016021,GO:0055085,GO:0005524 O06137 NC_000962.3 Mycobrowser_v5 CDS 1824430 1825887 . - 0 Rv1623c cydA Involved in the respiratory chain (at the terminal step): aerobic respiration. Cytochrome D terminal oxidase complex is the component of the aerobic respiratory chain that is supposedly predominant when cells are grown at low aeration [catalytic activity: ubiquinol-8 + O(2) = ubiquinone-8 + H(2)O]. Probable integral membrane cytochrome D ubiquinol oxidase (subunit I) CydA (cytochrome BD-I oxidase subunit I) Rv1623c, (MTCY01B2.15c), len: 485 aa. Probable cydA (previously known as appC, but renamed cydA to conform with Mycobacterium smegmatis nomenclature), cytochrome D ubiquinol oxidase subunit I, integral membrane protein, similar to others e.g. P26459|APPC_ECOLI|CYXA|CBDA|B0978 cytochrome BD-II oxidase subunit I from Escherichia coli strain K12 (514 aa), FASTA scores: opt: 870, E(): 0, (35.9% identity in 485 aa overlap); AL034355|SCD78_12 from Streptomyces coelicolor (501 aa), FASTA scores: opt: 1099, E(): 0, (48.6% identity in 510 aa overlap); etc. L7N662 No intermediary metabolism and respiration Q7D892 L7N662 GO:0055114,GO:0016491,GO:0016020 1.10.3.- NC_000962.3 Mycobrowser_v5 CDS 1826614 1827945 . - 0 Rv1625c cya Involved in cAMP synthesis [catalytic activity: ATP = 3',5'-cyclic AMP + diphosphate]. Inhibited by polyphosphates. Membrane-anchored adenylyl cyclase Cya (ATP pyrophosphate-lyase) (adenylate cyclase) Rv1625c, (MT1661, MTCY01B2.17c), len: 443 aa. Cya, membrane-anchored adenylyl cyclase (see citations below). C-terminal half is similar to region in numerous eukaryotic adenylate and guanylate cyclases. N-terminal half hydrophobic. FASTA score: CYG2_RAT|P22717 guanylate cyclase soluble, beta-2 chain (682 aa), FASTA scores: opt: 552, E(): 2.7e-26, (40.3% identity in 226 aa overlap). Some similarity to Rv2435c|MTCY428.11 from Mycobacterium tuberculosis (730 aa), E(): 7e-19. Start changed since first submission (+25 aa). Belongs to adenylyl cyclase class-4/guanylyl cyclase family. P9WQ35 No intermediary metabolism and respiration 1YK9 P0A4Y0 P9WQ35 GO:0004016,GO:0006171,GO:0016021,GO:0046872,GO:0005886,GO:0005524 4.6.1.1 P9WQ35 NC_000962.3 Mycobrowser_v5 CDS 1830665 1833379 . + 0 Rv1629 polA Involved in post-incision events. In addition to DNA polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [catalytic activity: N deoxynucleoside triphosphate = N diphosphate + {DNA}(N)]. Probable DNA polymerase I PolA Rv1629, (MTCY01B2.21), len: 904 aa. Probable polA, DNA polymerase I (see citations below). Has DNA polymerase family a signature (PS00447) at C-terminal end. FASTA best: DPO1_MYCTU|Q07700 DNA polymerase I from Mycobacterium tuberculosis (904 aa). Some similarity to Rv2090|MTCY49.30 (393 aa), E(): 2.2e-18, (38.7% identity in 292 aa overlap). Belongs to DNA polymerase type-a family. P9WNU5 No information pathways P0A550 P9WNU5 GO:0008409,GO:0003677,GO:0006281,GO:0006260,GO:0003887,GO:0005622,GO:0008408 2.7.7.7 P9WNU5 NC_000962.3 Mycobrowser_v5 CDS 1833542 1834987 . + 0 Rv1630 rpsA Binds mRNA; thus facilitating recognition of the initiation point. It is needed to translate mRNA with a short shine-dalgarno (SD) purine-rich sequence. 30S ribosomal protein S1 RpsA Rv1630, (MTCY01B2.22), len: 481 aa. rpsA, 30S ribosomal protein S1. FASTA best: RS1_MYCLE|P46836 30s ribosomal protein S1 from Mycobacterium leprae (482 aa), opt: 2655, E(): 0, (87.2% identity in 483 aa overlap). P9WH43 No information pathways O06147 P9WH43 GO:0005840,GO:0003735,GO:0006412,GO:0003723 P9WH43 NC_000962.3 Mycobrowser_v5 CDS 1837075 1839171 . + 0 Rv1633 uvrB Involved in nucleotide excision repair. The ABC excision nuclease is a DNA repair enzyme that catalyzes the excision reaction of UV-damaged nucleotide segments producing oligomers having the modified base(S). UVRB stimulates the ATPase activity of UVRA in the presence of UV-irradiated double-stranded DNA. It also enhances the ability of UVRA to bind to UV-irradiated duplex DNA Probable excinuclease ABC (subunit B - helicase) UvrB Rv1633, (MTCY01B2.25), len: 698 aa. Probable uvrB, excinuclease ABC, subunit B; helicase (see Mizrahi & Andersen 1998; Sancar 1994); has ATP/GTP-binding site motif A (P-loop; PS00017) near N-terminus (see citation below). FASTA best: UVRB_MICLU|P10125 from Micrococcus luteus (709 aa), opt: 3268, E(): 0, (71.3% identity in 704 aa overlap). Also similar to Mycobacterium tuberculosis Rv2973c (recG); and Rv1020 (mfd). Belongs to the UVRB family. P9WFC7 No information pathways P67422 P9WFC7 GO:0003677,GO:0009432,GO:0005737,GO:0009381,GO:0004386,GO:0006289,GO:0005524 P9WFC7 NC_000962.3 Mycobrowser_v5 CDS 1852273 1852878 . + 0 Rv1641 infC If-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins. Probable initiation factor if-3 InfC Rv1641, (MTCY06H11.05), len: 201 aa. Probable infC, initiation factor if-3, similar to many e.g. IF3_BACST|P03000 initiation factor if-3 from Bacillus stearothermophilus (171 aa), FASTA scores: opt: 560, E(): 1.9e-27, (50.6% identity in 166 aa overlap). Note that an AUC initiation codon has been used, the Bacillus (IF3_BACSU) and Escherichia coli (IF3_ECOLI) proteins use an AUU initiation codon, and the Myxococcus xanthus (DSG_MYXXA) homolog uses a AUC. Belongs to the if-3 family. P9WKJ9 No information pathways P65135 P9WKJ9 GO:0003743,GO:0006413,GO:0005737 P9WKJ9 NC_000962.3 Mycobrowser_v5 CDS 1859758 1862253 . + 0 Rv1650 pheT Charging PHE-tRNA [catalytic activity: ATP + L-phenylalanine + tRNA(PHE) = AMP + diphosphate + L-phenylalanyl-tRNA(PHE)] Probable phenylalanyl-tRNA synthetase, beta chain PheT Rv1650, (MTCY06H11.15), len: 831 aa. Probable pheT, Phenylalanyl-tRNA synthetase beta chain, similar to several e.g. SYFB_ECOLI|P07395 from Escherichia coli (795 aa), FASTA scores: opt: 995, E(): 0, (31.8% identity in 847 aa overlap). Belongs to the phenylalanyl-tRNA synthetase beta chain family - subfamily 1. P9WFU1 No information pathways P94985 P9WFU1 GO:0005737,GO:0000287,GO:0004826,GO:0006432,GO:0000049,GO:0008033,GO:0005524 6.1.1.20 P9WFU1 NC_000962.3 Mycobrowser_v5 CDS 1862347 1865382 . - 0 Rv1651c PE_PGRS30 Function unknown. Thought to be involved in virulence. PE-PGRS family protein PE_PGRS30 Rv1651c, (MTCY06H11.16c), len: 1011 aa. PE_PGRS30, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citations below), similar to many e.g. Q10637|Y03A_MYCTU hypothetical glycine-rich 49.6 kd protein (603 aa), FASTA scores: opt: 1757, E(): 0, (50.8% identity in 714aa overlap). The transcription of this CDS seems to be activated in macrophages (see Ramakrishnan et al., 2000). Q79FL8 No PE/PPE Q79FL8 Q79FL8 Q79FL8 NC_000962.3 Mycobrowser_v5 CDS 1870842 1871354 . + 0 Rv1657 argR Regulates arginine biosynthesis genes Probable arginine repressor ArgR (AHRC) Rv1657, (MTCY06H11.22), len: 170 aa. Probable argR, Arginine repressor (alternate gene name: ahrC). Similar to AHRC_BACSU|P17893 arginine hydroximate resistance protein from Bacillus subtilis (149 aa), FASTA scores: opt: 283, E(): 1.8e-11, (34.5% identity in 142 aa overlap); and ARGR_ECOLI|P15282 arginine repressor from Escherichia coli (156 aa), FASTA scores: opt: 194, E(): 6.4e-06, (30.8% identity in 146 aa overlap). Belongs to the ArgR family. P9WPY9 No regulatory proteins 2ZFZ,3BUE,3CAG,3ERE,3FHZ,3LAJ,3LAP P0A4Y8 P9WPY9 GO:0005737,GO:0006355,GO:0006350,GO:0003700,GO:0006526 P9WPY9 NC_000962.3 Mycobrowser_v5 CDS 1886512 1888020 . + 0 Rv1663 pks17 Potentially involved in some intermediate steps for the synthesis of a polyketide molecule which may be involved in secondary metabolism Probable polyketide synthase Pks17 Rv1663, (MTCY275.02), len: 502 aa. Probable pks17, polyketide synthase, similar to other polyketide synthases e g. ERY2_SACER|Q03132 erythronolide synthase, modules 3 and 4 (3567 aa) from Saccharopolyspora erythraea (Streptomyces erythraeus), FASTA scores: opt: 1207, E(): 0, (43.9% identity in 531 aa overlap). Also similar to other Mycobacterium tuberculosis probable polyketide synthases e.g. pks7 and pks1. Note that the similarity extends into the upstream ORF Rv1662 (MTCY275.01) and this could be accounted for by a frameshift, although the sequence has been checked and no discrepancy was found. Contains PS00012 Phosphopantetheine attachment site. O06585 No lipid metabolism O06585 O06585 GO:0009058,GO:0048037,GO:0016491,GO:0031177,GO:0000036 O06585 NC_000962.3 Mycobrowser_v5 CDS 1891226 1892287 . + 0 Rv1665 pks11 Possibly involved in the biosynthesis of secondary metabolites [catalytic activity: 3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2] Chalcone synthase Pks11 Rv1665, (MTCY275.04-MTV047.01), len: 353 aa. pks11, chalcone synthase, some similarity to BCSA_BACSU|P54157 putative chalcone synthase from Bacillus subtilis (365 aa), FASTA scores: opt: 615, E(): 6.2e-32, (33.4% identity in 308 aa overlap); and to many plant chalcone synthases e.g. CHS_VIGUN|P51089 chalcone synthase (388 aa), FASTA scores: opt: 391, E(): 7.8e-18, (27.2% identity in 349 aa overlap). Highly similar to upstream ORF Rv1660|MTCY06H11.25 pks10 (72.7% identity in 308 aa overlap); and Rv1372 pks18. P9WPF3 No lipid metabolism O06587 P9WPF3 GO:0009058,GO:0016210,GO:0008415 2.3.1.74 P9WPF3 NC_000962.3 Mycobrowser_v5 CDS 1896876 1898207 . - 0 Rv1672c Rv1672c Thought to be involved in transport across the membrane. Responsible for the translocation of undetermined substrate across the membrane. Probable conserved integral membrane transport protein Rv1672c, (MTV047.07c), len: 443 aa. Probable conserved integral membrane transport protein, major facilitator superfamily, similar to several phthalate transporters or tartrate transporters e.g. U25634|AVU25634_2 Agrobacterium vitis plasmid pTrAB (433 aa), FASTA scores: opt: 914, E(): 0, (37.1% identity in 426 aa overlap); etc. O53919 No cell wall and cell processes O53919 O53919 GO:0055085 NC_000962.3 Mycobrowser_v5 CDS 1903299 1904420 . + 0 Rv1679 fadE16 Function unknown, but involvement in lipid degradation. Possible acyl-CoA dehydrogenase FadE16 Rv1679, (MTV047.14, MTCI125.01), len: 373 aa. Possible fadE16, acyl-CoA dehydrogenase, similar to acyl/butyryl-CoA dehydrogenases e.g. NP_244665.1|NC_002570 acyl-CoA dehydrogenase from Bacillus halodurans (380 aa); NP_000008.1|NM_000017 acyl-Coenzyme A dehydrogenase from Homo sapiens (412 aa); Z99113|BSUB0010_119 from Bacillus subtilis (380 aa), FASTA scores: opt: 439, E(): 3.4e-20, (29.6% identity in 287 aa overlap); etc. Weakly similar to many dehydrogenases and to P31571|CAIA_ECOLI probable carnitine operon oxidoreductase from Escherichia coli (380 aa), FASTA scores: opt: 109, E(): 0.0066, (28.6% identity in 98 aa overlap). O53926 No lipid metabolism O53926 O53926 GO:0003995,GO:0055114,GO:0050660 1.3.99.- O53926 NC_000962.3 Mycobrowser_v5 CDS 1907594 1910593 . + 0 Rv1683 Rv1683 Supposed involvement in lipid biosynthesis and degradation Possible bifunctional enzyme; long-chain acyl-CoA synthase and lipase. Rv1683, (MTCI125.05), len: 999 aa. Possible bifunctional long-chain acyl-CoA synthase and lipase. Equivalent to Z95117|MLCB1351_21 possible long-chain acyl-CoA synthase from Mycobacterium leprae (1002 aa) (85.6% identity in 1002 aa overlap). Weakly similar to FATP_MOUSE|Q60714 long-chain fatty acid transport protein (646 aa), fasta scores: opt: 331, E(): 5e-08, (24.8% identity in 630 aa overlap). Also similar to O35488|AF033031 Mouse very-long-chain acyl-CoA synthetase (620 aa), fasta scores: opt: 435, E(): 2.2e-12, (24.8% identity in 545 aa overlap). Weakly similar to Mycobacterium tuberculosis protein MTCI364.18 (27.4% identity in 583 aa overlap). Contains PS00120 Lipases, serine active site. O33185 No lipid metabolism O33185 O33185 GO:0008152,GO:0003824 O33185 NC_000962.3 Mycobrowser_v5 CDS 1912153 1912920 . - 0 Rv1687c Rv1687c Thought to be involved in active transport of undetermined substrate (possibly drug) across the membrane. Responsible for energy coupling to the transport system. Probable conserved ATP-binding protein ABC transporter Rv1687c, (MTCI125.09c), len: 255 aa. Probable conserved ATP-binding protein ABC transporter (see citation below), similar to many ABC-type transporters e.g. P55476|NODI_RHISN nodulation ATP-binding protein I from Rhizobium sp. (343 aa), FASTA scores: opt: 479, E(): 3.7e-23, (34.6% identity in 243 aa overlap); etc. Also similar to many other Mycobacterium tuberculosis ABC-type transporters e.g. MTCY19H9.04 (34.5% identity in 238 aa overlap). Contains PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters). Also contains PS00039 dead-box subfamily ATP-dependent helicases signature, though this may be spurious. O33189 No cell wall and cell processes O33189 O33189 GO:0016887,GO:0005524 O33189 NC_000962.3 Mycobrowser_v5 CDS 1917940 1918746 . + 0 Rv1694 tlyA Methylates 16S and 23S rRNA. Also has contact-dependent haemolytic activity; possibly involved in virulence (pore formation). 2'-O-methyltransferase TlyA Rv1694, (MTCI125.16), len: 268 aa. TlyA, 2'-O-methyltransferase; cytotoxin/haemolysin homologue (see citations below), almost identical to NP_301968.1|NC_002677 cytotoxin/haemolysin homologue TlyA from Mycobacterium leprae (269 aa). TlyA homologues were also identified by PCR in Mycobacterium avium, Mycobacterium bovis BCG, but appeared absent in M. smegmatis, M. vaccae, M. kansasii, M. chelonae and M. phlei (see Wren et al., 1998). Also highly similar to CAB83047.1|AJ271681 putative haemolysin from Mycobacterium ulcerans (281 aa); and similar to HLYA_TREHY|Q06803 pore-forming haemolysin/cytotoxin virulence determinant from Treponema hyodysenteriae (240 aa), FASTA scores: opt: 514, E():3e-30, (37.3% identity in 236 aa overlap). P9WJ63 No virulence, detoxification, adaptation Q50760 P9WJ63 GO:0019836,GO:0032259,GO:0008168,GO:0003723 AMI P9WJ63 NC_000962.3 Mycobrowser_v5 CDS 1929131 1929721 . - 0 Rv1703c Rv1703c Catalyzes the O-methylation [catalytic activity: S-adenosyl-L-methionine + catechol = S-adenosyl-L-homocysteine + guaiacol] Probable catechol-O-methyltransferase Rv1703c, (MTCI125.25c), len: 196 aa. Probable catechol-o-methyltransferase, most similar to COMT_HUMAN|P21964 soluble form of mammalian catechol o-methyltransferase (271 aa), FASTA scores: opt: 405, E(): 7.8e-29, (38.9% identity in 190 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical methyltransferases Rv0187, Rv1220c. L0TAD5 No intermediary metabolism and respiration O33202 L0TAD5 GO:0016206 2.1.1.6 L0TAD5 NC_000962.3 Mycobrowser_v5 CDS 1929786 1931456 . - 0 Rv1704c cycA Permease that is involved in the transport across the cytoplasmic membrane of D-alanine, D-serine and glycine Probable D-serine/alanine/glycine transporter protein CycA Rv1704c, (MTCI125.26c), len: 556 aa. Probable cycA, D-serine/D-alanine/glycine transporter, highly similar to P39312|CYCA_ECOLI d-serine/d-alanine/glycine transporter from Escherichia coli (470 aa), FASTA scores: opt: 1906, E(): 0, (59.3% identity in 459 aa overlap); etc. Also similar to other Mycobacterium tuberculosis amino-acid permeases e.g. Rv2127, Rv0346c, etc. Contains PS00218 amino acid permeases signature. Belongs to the amino acid permease family (APC family). O33203 No cell wall and cell processes O33203 O33203 GO:0006865,GO:0016021,GO:0055085,GO:0015171 O33203 NC_000962.3 Mycobrowser_v5 CDS 1934882 1936342 . + 0 Rv1707 Rv1707 Unknown; possibly involved in transport of sulfate across the membrane. Probable conserved transmembrane protein Rv1707, (MTCI125.29), len: 486 aa. Probable conserved transmembrane protein, possibly involved in transport of sulfate, similar to several hypothetical proteins belonging to the sulfate permease family e.g. P40877|YCHM_ECOLI hypothetical 58.4 kDa protein in pth-prsa intergenic region from Escherichia coli (550 aa), FASTA scores: opt: 486, E(): 0, (33.1% identity in 492 aa overlap). Also similar to many other Mycobacterium tuberculosis membrane proteins e.g. Rv3273, Rv1739c. Seems to belong to the SulP family. O33206 No cell wall and cell processes O33206 O33206 GO:0055085,GO:0005215,GO:0016021 O33206 NC_000962.3 Mycobrowser_v5 CDS 1942659 1943573 . + 0 Rv1715 fadB3 Thought to be involved in fatty acid degradation. FADB and FADA are the alpha and beta subunits of the multifunctional enzyme complex of the fatty acid degradation cycle [catalytic activity: (S)-3-hydroxybutanoyl-CoA + NADP+ = 3-acetoacetyl-CoA + NADPH]. Probable 3-hydroxybutyryl-CoA dehydrogenase FadB3 (beta-hydroxybutyryl-CoA dehydrogenase) (BHBD) Rv1715, (MTV048.02), len: 304 aa. Probable fadB3, 3-hydroxybutyryl-CoA dehydrogenase, highly similar to many e.g. NP_107236.1|NC_002678 3-hydroxybutyryl-CoA dehydrogenase from Mesorhizobium loti (309 aa); NP_250319.1|NC_002516 probable 3-hydroxyacyl-CoA dehydrogenase from Pseudomonas aeruginosa (509 aa); P45856|HBD_BACSU probable 3-hydroxybutyryl-CoA dehydrogenase from Bacillus subtilis (287 aa), FASTA scores: opt: 488, E(): 1.5e-24, (38.7% identity in 279 aa overlap); etc. Could belong to the 3-hydroxyacyl-CoA dehydrogenase family. L7N688 No lipid metabolism Q7D836 L7N688 GO:0050662,GO:0006631,GO:0055114,GO:0008691 1.1.1.157 L7N688 NC_000962.3 Mycobrowser_v5 CDS 3004745 3005650 . - 0 Rv2688c Rv2688c Thought to be involved in active transport of unidentified antibiotic across the membrane (export): antibiotic resistance by an export mechanism. Responsible for energy coupling to the transport system. Antibiotic-transport ATP-binding protein ABC transporter Rv2688c, (MTCY05A6.09c), len: 301 aa. Antibiotic-transport ATP-binding protein ABC transporter (see citation below), highly similar to AAK47077|MT2762 ABC transporter ATP-binding protein from Mycobacterium tuberculosis strain CDC1551 (317 aa), FASTA scores: opt: 1714, E(): 5.1e-93, (95.6% identity in 274 aa overlap). Also highly similar to other ATP-binding proteins ABC transporter e.g. Q9K639|BH3893 from Bacillus halodurans (282 aa), FASTA scores: opt: 644, E(): 1.4e-30, (38.% identity in 285 aa overlap); O58550|PH0820 from Pyrococcus horikoshii (312 aa), FASTA scores: opt: 574, E(): 1.8e-26, (39.1% identity in 307 aa overlap); Q9WYM0|TM0389 from Thermotoga maritima (301 aa), FASTA scores: opt: 536, E(): 2.9e-24, (36.1% identity in 291 aa overlap); etc. Has ATP/GTP-binding site motif A (P-loop) at N-terminus (PS00017). Belongs to the ATP-binding transport protein family (ABC transporters). P9WQL7 No cell wall and cell processes O07190 P9WQL7 GO:0016887,GO:0005886,GO:0046677,GO:0006810,GO:0005524 3.6.3.- P9WQL7 NC_000962.3 Mycobrowser_v5 CDS 1954631 1955569 . - 0 Rv1729c Rv1729c Possible methyltransferase Possible S-adenosylmethionine-dependent methyltransferase Rv1729c, (MTCY04C12.14c), len: 312 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), similar to many Mycobacterium tuberculosis proteins e.g. Q50726|Rv3399|YX99_MYCTU (348 aa), FASTA scores: opt: 1019, E(): 0, (55.7% identity in 296 aa overlap); P95074|Rv0726c (367 aa), O53795|Rv0731c (318 aa), and O53841|Rv0830 (301 aa), etc. P9WFH9 No lipid metabolism P71987 P9WFH9 GO:0008168 2.1.1.- P9WFH9 NC_000962.3 Mycobrowser_v5 CDS 1957677 1959233 . + 0 Rv1731 gabD2 Involved in 4-aminobutyrate (GABA) degradation pathway [catalytic activity: succinate semialdehyde + NAD(P)(+) + H(2)O = succinate + NAD(P)H]. Possible succinate-semialdehyde dehydrogenase [NADP+] dependent (SSDH) GabD2 Rv1731, (MTCY04C12.16), len: 518 aa. Possible gabD2, succinate-semialdehyde dehydrogenase [NADP+] dependent, similar to others e.g. GABD_ECOLI|P25526 succinate-semialdehyde dehydrogenase from Escherichia coli (482 aa), FASTA scores: opt: 870, E(): 0, (34.7% identity in 449 aa overlap); etc. Also similar to gabD1|Rv0234c|MTCY08D5.30c probable succinate-semialdehyde dehydrogenase [NADP+] dependent from Mycobacterium tuberculosis (511 aa); and other semialdehyde dehydrogenases e.g. Rv0768|aldA (489 aa), Rv2858c|aldC (455 aa), etc. Contains PS00216 Sugar transport proteins signature 1, PS00687 Aldehyde dehydrogenases glutamic acid active site. Belongs to the aldehyde dehydrogenases family. Note that previously known as gabD1. P9WNX7 No intermediary metabolism and respiration P96417 P9WNX7 GO:0009013,GO:0055114 1.2.1.16 P9WNX7 NC_000962.3 Mycobrowser_v5 CDS 1962228 1964186 . - 0 Rv1736c narX Involved in nitrate reduction, and in the persistence in the host [catalytic activity: nitrite + acceptor = nitrate + reduced acceptor] Probable nitrate reductase NarX Rv1736c, (MTCY04C12.21c), len: 652 aa. Probable narX, nitrate reductase. Contains three domains: N-terminus (250 aa) is similar to e.g. N-terminus of NARG_ECOLI|P09152 respiratory nitrate reductase 1 alpha chain from Escherichia coli (1246 aa), FASTA scores: E(): 0, (58.6% identity in 251 aa overlap); and Rv1161|MTCI65.28|NARG probable respiratory nitrate reductase (alpha chain) from Mycobacterium tuberculosis (1232 aa). Central region (260-410 aa) is similar to Rv1163|O06561|NARJ probable respiratory nitrate reductase (delta chain) from Mycobacterium tuberculosis (201 aa), FASTA scores: E(): 0, (64.2% identity in 159 aa overlap). C-terminus (420 aa-) is similar to Rv1164|O06562|NARI probable respiratory nitrate reductase (gamma chain) from Mycobacterium tuberculosis (246 aa), FASTA scores: E(): 0, (68.6% identity in 239 aa overlap). Contains PS00551 Prokaryotic molybdopterin oxidoreductases signature 1. P9WJQ1 No intermediary metabolism and respiration P71994 P9WJQ1 GO:0009055,GO:0022900,GO:0016021,GO:0046872,GO:0042128,GO:0008940,GO:0009325,GO:0005886,GO:0006810,GO:0051539 1.7.99.4 P9WJQ1 NC_000962.3 Mycobrowser_v5 CDS 1964183 1965370 . - 0 Rv1737c narK2 Involved in excretion of nitrite, produced by the dissimilatory reduction of nitrate, across the membrane. Responsible for the translocation of the substrate across the membrane. Possible nitrate/nitrite transporter NarK2 Rv1737c, (MTCY04C12.22c), len: 395 aa. Possible narK2, nitrate/nitrite-transport integral membrane protein (see Hutter & Dick 2000), possibly member of major facilitator superfamily (MFS), similar to P46907|NARK_BACSU nitrite extrusion protein from Bacillus subtilis (395 aa), FASTA scores: opt: 742, E(): 0, (33.6% identity in 375 aa overlap); and to AL109989|SCJ12.23 hypothetical nitrate/nitrite transporter from Streptomyces coelicolor (412 aa), FASTA scores: opt: 1181, E(): 0, (49.4% identity in 389 aa overlap). P9WJY7 No cell wall and cell processes P71995 P9WJY7 GO:0042128,GO:0005886,GO:0055085,GO:0016021 P9WJY7 NC_000962.3 Mycobrowser_v5 CDS 1965955 1967637 . - 0 Rv1739c Rv1739c Involved in sulphate transport across the membrane. Responsible for the translocation of the substrate across the membrane. Probable sulphate-transport transmembrane protein ABC transporter Rv1739c, (MTCY04C12.24c, MTCY28.01), len: 560 aa. Probable sulphate-transport transmembrane protein ABC transporter, similar to several e.g. P53392|G607186 high affinity sulphate transporter from Stylosanthes hamata (662 aa), FASTA scores: opt: 382, E(): 1.6e-16, (28.0% identity in 564 aa overlap); U59234.1|AAB88215.1 biotin carb. from Synechococcus sp. PCC 7942 (574 aa), FASTA scores: opt: 1838, E(): 0, (50.0% identity in 550 aa overlap); etc. Contains PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters), and seems to belong to the SULP family. P9WGF7 No cell wall and cell processes P71997 P9WGF7 GO:0005886,GO:0008271,GO:0008272,GO:0055085,GO:0016021 P9WGF7 NC_000962.3 Mycobrowser_v5 CDS 3034635 3034883 . + 0 Rv2722 Rv2722 Function unknown Conserved hypothetical protein Rv2722, (MTCY154.02), len: 82 aa. Conserved hypothetical protein, similar to Q9CCB9|ML1001 hypothetical protein from Mycobacterium leprae (91 aa), FASTA scores: opt: 154, E(): 0.00053, (37.5% identity in 88 aa overlap). Equivalent to AAK47111 from Mycobacterium tuberculosis strain CDC1551 (94 aa) but shorter 12 aa. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O33227 No conserved hypotheticals O33227 NC_000962.3 Mycobrowser_v5 CDS 1969004 1970704 . + 0 Rv1743 pknE Involved in signal transduction (via phosphorylation). Thought to be involved in membrane transport [catalytic activity: ATP + a protein = ADP + a phosphoprotein]. Probable transmembrane serine/threonine-protein kinase E PknE (protein kinase E) (STPK E) Rv1743, (MTCY28.05,MTCY04C12.28), len: 566 aa. Probable pknE, transmembrane serine/threonine protein kinase (see citation below), similar to PKN1_MYXXA|P33973 serine/threonine-protein kinase pkn1 (693 aa), fasta scores: opt: 542, E(): 1.1e-19, (35.8% identity in 302 aa overlap). Also highly similar to K08G_MYCTU|Q11053 probable serine/threonine-protein kinase (626 aa) (59.8% identity in 381 aa overlap). Contains PS00107 Protein kinases ATP-binding region signature. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases. P9WI77 No regulatory proteins 2H34 P72001 P9WI77 GO:0016021,GO:0005886,GO:0006468,GO:0004674,GO:0005524 2.7.11.1 P9WI77 NC_000962.3 Mycobrowser_v5 CDS 1977969 1979567 . - 0 Rv1750c fadD1 Function unknown, but supposed involvement in lipid degradation. Possible fatty-acid-CoA ligase FadD1 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv1750c, (MTCY28.13c, MTCY04C12.34), len: 532 aa. Possible fadD1, fatty-acid-CoA synthetase, similar in part to others e.g. O35488|VLCS_MOUSE very-long-chain acyl-CoA synthetase from Mus musculus (620 aa); NP_113924.1|NM_031736 solute carrier family 27 (fatty acid transporter) member 2 from Rattus norvegicus (620 aa); NP_459076.1|NC_003197 crotonobetaine/carnitine-CoA ligase from Salmonella typhimurium (517 aa); CAIC_ECOLI|P31552 probable crotonobetaine/carnitine-CoA ligase from Escherichia coli (522 aa), FASTA scores: opt: 448, E(): 1.9e-21, (25.1% identity in 502 aa overlap); etc. Also highly similar to fadD17|Rv3506|MTV023.13 probable fatty-acid-CoA ligase from Mycobacterium tuberculosis (502 aa); and similar to others from Mycobacterium tuberculosis e.g. fadD6|MTCI364.18|Rv1206|O05307 probable fatty-acid-CoA ligase (597 aa), FASTA score: (28.3% identity in 519 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family. P72007 No lipid metabolism P72007 P72007 GO:0008152,GO:0016874 6.2.1.- P72007 NC_000962.3 Mycobrowser_v5 CDS 1988680 1989006 . - 0 Rv1757c Rv1757c Required for the transposition of the insertion element IS6110. Putative transposase for insertion sequence element IS6110 (fragment) Rv1757c, (MTCY28.23c), len: 108 aa. Putative Transposase for IS6110 (fragment), identical to many other Mycobacterium tuberculosis IS6110 transposase subunits e.g. Q50686|YIA4_MYCTU Insertion element IS6110 hypothetical 12.0 kDa protein (108 aa), fasta scores: E(): 1.4e-43, (100.00% identity in 108 aa overlap). The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv1756c and Rv1757c, the sequence UUUUAAAG (directly upstream of Rv1756c) maybe responsible for such a frameshifting event (see McAdam et al., 1990). P9WKH5 No insertion seqs and phages P0C5G9 P9WKH5 GO:0004803,GO:0006313,GO:0003677 P9WKH5 NC_000962.3 Mycobrowser_v5 CDS 3430387 3430710 . + 0 Rv3065 mmr Involved in transport of multidrugs (tetraphenylphosphonium, erythromycin, ethidium bromide, acriflavine, safranin O, pyronin Y, etc) across the membrane (export): multidrugs resistance by an export mechanism (conferes resistance to toxic compounds by removing them for the cells). Responsible for the translocation of the substrate across the membrane. Multidrugs-transport integral membrane protein Mmr Rv3065, (MT3150.1, MTCY22D7.17c), len: 107 aa. Mmr, integral membrane multidrugs resistance transporter (see citation below), equivalent to Q9CBP1|ML1756 probable multidrug resistance protein from Mycobacterium leprae (107 aa), FASTA scores: opt: 534, E(): 3.3e-28, (77.55% identity in 107 aa overlap). Also highly similar to bacterial proteins involved in resistance to ethidium bromide or methyl viologen e.g. O87866|QACG_STASP quaternary ammonium compound-resistance protein QACG (quarternary ammonium determinant G) from Staphylococcus sp. strain ST94 (107 aa), FASTA scores: opt: 307, E(): 1.8e-13, (39.8% identity in 103 aa overlap); P96460|QAC quaternary ammonium compounds resistance protein QAC from Staphylococcus aureus (107 aa), FASTA scores: opt: 304, E(): 2.8e-13, (40.4% identity in 104 aa overlap); Q57225|QACE_ECOLI quaternary ammonium compound-resistance protein QACE (quarternary ammonium determinant E) from Escherichia coli (110 aa), FASTA scores: opt: 300, E(): 5.2e-13, (48.15% identity in 108 aa overlap); AAG55967|Z1870 methylviologen resistance protein encoded within prophage CP-933X from Escherichia coli strain O157:H7 EDL933 (110 aa); P23895|EMRE|MVRC|EB|B0543 EMRE protein from Escherichia coli (110 aa), FASTA scores: opt: 290, E(): 2.3e-12, (43.55% identity in 101 aa overlap); etc. Also similar to the SugE protein of enteric bacteria. Belongs to the small multidrug resistance (SMR) protein family. Note that previously known as emrE. P9WGF1 No cell wall and cell processes 2IQ4 P69926 P9WGF1 GO:0005886,GO:0046677,GO:0006810,GO:0016021 P9WGF1 NC_000962.3 Mycobrowser_v5 CDS 1989833 1992577 . - 0 Rv1759c wag22 Function unknown. Has fibronectin-binding activity (could thus mediate bacterial attachment to host cells). Thought to be expressed during infection. PE-PGRS family protein Wag22 Rv1759c, (MT1807, MTCY28.25c), len: 914 aa. Wag22, antigen member (see citations below) of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins, highly similar to others e.g. MT1367|Q10637 hypothetical glycine-rich 49.6 kDa protein from Mycobacterium tuberculosis (603 aa), FASTA scores: opt: 2010, E(): 0, (53.0% identity in 724 aa overlap); etc. P9WIG5 No PE/PPE P0A686 P9WIG5 P9WIG5 NC_000962.3 Mycobrowser_v5 CDS 1996427 1997413 . + 0 Rv1764 Rv1764 Required for the transposition of the insertion element IS6110. Putative transposase Rv1764, (MTCY28.30), len: 328 aa. Putative Transposase for IS6110 insertion element. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv1763 and Rv1764, the sequence UUUUAAAG (directly upstream of Rv1764) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 34 aa) P9WKH9 No insertion seqs and phages P0C5G8 P9WKH9 GO:0015074,GO:0006310,GO:0032196,GO:0003677 P9WKH9 NC_000962.3 Mycobrowser_v5 CDS 2005161 2006447 . + 0 Rv1771 Rv1771 Possibly involved in biosynthesis of L-ascorbic acid (vitamin C). Oxidizes L-gulono-1,4-lactone. L-gulono-1,4-lactone dehydrogenase Rv1771, (MTCY28.37), len: 428 aa. L-gulono-1,4-lactone dehydrogenase (See Wolucka and Communi, 2006), similar to e.g. GGLO_RAT|P10867 l-gulonolactone oxidase (439 aa), FASTA scores: opt: 862, E(): 0, (34.1% identity in 434 aa overlap). Also shows slight similarity to Mycobacterium tuberculosis oxidoreductase Rv1726|MTCY04C12.11 (22.9% identity in 441 aa overlap) and others e.g. Rv3107c, Rv1257c, Rv2251, etc. Contains PS00862 Oxygen oxidoreductases covalent FAD-binding site. Alternative nucleotide at position 2006032 (a->G; Q291R) has been observed. P9WIT3 No intermediary metabolism and respiration O06804 P9WIT3 GO:0050660,GO:0016020,GO:0055114,GO:0003885 1.-.-.- P9WIT3 NC_000962.3 Mycobrowser_v5 CDS 2010656 2011960 . + 0 Rv1777 cyp144 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Probable cytochrome P450 144 Cyp144 Rv1777, (MT1827, MTCY25C11.04), len: 434 aa. Probable cyp144, cytochrome p450, similar to CPXM_BACME|Q06069 cytochrome p450 (meg) (410 aa), FASTA scores: opt: 435 E(): 2.3e-16, (28.8% identity in 372 aa overlap). Also similar to several other Mycobacterium tuberculosis p450 genes including Rv0766c, Rv2266, etc. Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. Belongs to the cytochrome P450 family. P9WPL1 No intermediary metabolism and respiration O33180 P9WPL1 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPL1 NC_000962.3 Mycobrowser_v5 CDS 2015302 2017476 . - 0 Rv1781c malQ Transfers a segment of a (1,4)-alpha-D-glucan to a new 4-position in an acceptor, which may be glucose or (1,4)-alpha-D-glucan Probable 4-alpha-glucanotransferase MalQ (amylomaltase) (disproportionating enzyme) (D-enzyme) Rv1781c, (MTV049.03c), len: 724 aa. Probable malQ, 4-alpha-glucanotransferase, similar to many, e.g. P15977|MALQ_ECOLI 4-alpha-glucanotransferase (694 aa), FASTA scores: opt: 964, E(): 0, (31.8% identity in 694 aa overlap). Belongs to the disproportionating enzyme family. P9WK23 No intermediary metabolism and respiration P65336 P9WK23 GO:0005975,GO:0043169,GO:0005737,GO:0004134 2.4.1.25 P9WK23 NC_000962.3 Mycobrowser_v5 CDS 2028425 2029477 . + 0 Rv1790 PPE27 Function unknown PPE family protein PPE27 Rv1790, (MTV049.12), len: 350 aa. PPE27, Member of the Mycobacterium tuberculosis PPE family of glycine-rich protein, similar to Z74024|MTCY274.24 Mycobacterium tuberculosis cosmid (404 aa), FASTA scores: opt: 849, E(): 0, (50.0% identity in 406 aa overlap). Q79FK5 No PE/PPE Q79FK5 Q79FK5 Q79FK5 NC_000962.3 Mycobrowser_v5 CDS 3709715 3710248 . - 0 Rv3324c moaC3 Thought to be involved in the biosynthesis of molybdopterin. Probable molybdenum cofactor biosynthesis protein C 3 MoaC3 Rv3324c, (MTV016.24c), len: 177 aa. Probable moaC3, molybdopterin cofactor biosynthesis protein, highly similar to others e.g. Q9HX95|MOAC|PA3918 from Pseudomonas aeruginosa (160 aa), FASTA scores: opt: 567, E(): 7.5e-30, (58.35% identity in 156 aa overlap); Q9RKA8|MOAC from Streptomyces coelicolor (170 aa), FASTA scores: opt: 553, E(): 6.3e-29, (58.25% identity in 158 aa overlap); P30747|MOAC_ECOLI|CHLA3|B0783 from Escherichia coli strain K12 (160 aa), FASTA scores: opt: 516, E(): 1.5e-26, (55.95% identity in 159 aa overlap); etc. Also highly similar to O05788|MOAC1|Rv3111|MTCY164.21 putative molybdenum cofactor biosynthesis protein C from Mycobacterium tuberculosis (170 aa), FASTA scores: opt: 734, E(): 1.3e-40, (71.8% identity in 163 aa overlap); and Rv0864|MOAC2|MTV043.57 putative molybdenum cofactor biosynthesis protein (167 aa). P9WJR5 No intermediary metabolism and respiration P65392 P9WJR5 GO:0006777 P9WJR5 NC_000962.3 Mycobrowser_v5 CDS 2031066 2031968 . + 0 Rv1794 Rv1794 Function unknown Conserved protein Rv1794, (MTV049.16), len: 300 aa. Conserved protein, slight similarity to Mycobacterium tuberculosis O53694|Rv0289|MTV035.17, (295 aa), FASTA scores: opt: 172, E(): 0.00083, (25.7% identity in 261 aa overlap). Equivalent to Mycobacterium leprae hypothetical protein Q9Z5I1|MLCB596.31|AL035472 (300 aa), (88.0% identity in 300 aa overlap). Contains PS00211 ABC transporters family signature. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53943 No conserved hypotheticals O53943 O53943 NC_000962.3 Mycobrowser_v5 CDS 2033729 2035486 . + 0 Rv1796 mycP5 Thought to have proteolytic activity. Probable proline rich membrane-anchored mycosin MycP5 (serine protease) (subtilisin-like protease) (subtilase-like) (mycosin-5) Rv1796, (MTV049.18), len: 585 aa. Probable mycP5, pro-rich membrane-anchored serine protease (mycosin) (see citations below). Member of family with four other Mycobacterium tuberculosis serine proteases: Rv3886c|O05458|MTCY15F10.26|Z94121 (550 aa), FASTA scores: opt: 1173, E(): 0, (47.9% identity in 578 aa overlap); Rv0291, Rv3883c, and Rv3449. Genes all linked to those of ESAT-6 family. Has possible N-terminal signal peptide and hydrophobic anchor-like stretch at C-terminus. Contains two serine protease, subtilase family active site motifs: a aspartic acid active site motif (PS00136); and a histidine active site motif (PS00137). Belongs to peptidase family S8 (also known as the subtilase family), pyrolysin subfamily. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). O53945 No intermediary metabolism and respiration O53945 O53945 GO:0004252,GO:0006508 3.4.21.- O53945 NC_000962.3 Mycobrowser_v5 CDS 2035483 2036703 . + 0 Rv1797 eccE5 Function unknown ESX conserved component EccE5. ESX-5 type VII secretion system protein. Probable membrane protein. Rv1797, (MTV049.19), len: 406 aa. eccE5, esx conserved component, ESX-5 type VII secretion system protein, probable membrane protein, some similarity to Mycobacterium tuberculosis O05462|Rv3882c|MTCY15F10.30|Z94121 (462 aa), FASTA scores: opt: 181, E(): 9.2e-05, (25.4% identity in 283 aa overlap). Has two hydrophobic stretch near N-terminus. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WJE3 No cell wall and cell processes O53946 P9WJE3 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2036700 2038532 . + 0 Rv1798 eccA5 Function unknown ESX conserved component EccA5. ESX-5 type VII secretion system protein. Rv1798, (MTV049.20), len: 610 aa. eccA5, esx conserved component, ESX-5 type VII secretion system protein, similar to several mycobacterial proteins e.g. O05460|MTCY15F10.28|Rv3884c|Z94121 from M. tuberculosis (619 aa), FASTA scores: opt: 669, E(): 0, (31.0% identity in 549 aa overlap); and O33089|MLCB628.18c|Y14967 from Mycobacterium leprae (573 aa), FASTA scores: opt: 723, E(): 0, (32.4% identity in 568 aa overlap). Also very similar to Rv0282. May belong to the CbxX/CfqX family as last ~320 aa domain very similar to several family members. Contains ATP/GTP-binding site motif A (P-loop; PS00017). P9WPI1 No cell wall and cell processes P63744 P9WPI1 GO:0005737,GO:0017111,GO:0005524 P9WPI1 NC_000962.3 Mycobrowser_v5 CDS 2044923 2046842 . - 0 Rv1803c PE_PGRS32 Function unknown PE-PGRS family protein PE_PGRS32 Rv1803c, (MTV049.25c), len: 639 aa. PE_PGRS32, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below). Most similar to Rv1768|MTCY28.34|Z95890 (618 aa), FASTA scores: opt: 1827, E(): 0, (53.5% identity in 664 aa overlap). Contains two PS00583 pfkB family of carbohydrate kinases signatures 1. Predicted to be an outer membrane protein (See Song et al., 2008). Q79FJ9 No PE/PPE Q79FJ9 Q79FJ9 NC_000962.3 Mycobrowser_v5 CDS 2051282 2052688 . + 0 Rv1809 PPE33 Function unknown PPE family protein PPE33 Rv1809, (MTV049.31), len: 468 aa. PPE33, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, most similar to RV1802AL022021|MTV049.23 (463 aa), FASTA scores: opt: 1238, E(): 0, (51.2% identity in 471 aa overlap). Alternative nucleotide at position 2051746 (T->C; A155A) has been observed. P9WI03 No PE/PPE Q79FJ5 P9WI03 P9WI03 NC_000962.3 Mycobrowser_v5 CDS 2056521 2057423 . + 0 Rv1814 erg3 Involved in lipid desaturation Membrane-bound C-5 sterol desaturase Erg3 (sterol-C5-desaturase) Rv1814, (MTCY1A11.29c), len: 300 aa. Erg3, transmembrane C-5 sterol desaturase (see *), weak similarity to several e.g. ERG3_YEAST|P32353 c-5 sterol desaturase (365 aa), FASTA scores: opt: 154, E(): 0.0011, (22.9% identity in 288 aa overlap). Belongs to the sterol desaturase family. [* Note: work of Jackson, C.J., Lamb, D.C., Kelly, D.E., Kelly, S.L., Characterization of a sterol delta 5,6-desaturase homolog in Mycobacterium bovis (BCG). Submitted (jun-2000) to the EMBL/GenBank/DDBJ databases]. P9WNZ9 No lipid metabolism P68435 P9WNZ9 GO:0006633,GO:0016021,GO:0005506,GO:0055114,GO:0016491,GO:0005886,GO:0016126,GO:0005783 1.3.-.- NC_000962.3 Mycobrowser_v5 CDS 2062809 2064728 . - 0 Rv1819c bacA Thought to be involved in active transport of drugs across the membrane (export): multidrugs resistance by an export mechanism. Responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane. Probable drug-transport transmembrane ATP-binding protein ABC transporter BacA Rv1819c, (MTCY1A11.24), len: 639 aa. Probable bacA, drug-transport transmembrane ATP-binding protein ABC transporter (see citation below), equivalent to AL008609|MLCB1788.47 hypothetical ABC transporter from Mycobacterium leprae (638 aa), (74.9% identity in 634 aa overlap). Also similar to other transmembrane ATP-binding proteins e.g. Q57335|Y036_HAEIN hypothetical ABC transporter ATP-binding protein from Haemophilus influenzae (592 aa), FASTA scores: opt: 1235, E(): 2.8e-61, (40.8% identity in 623 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). P9WQI9 No cell wall and cell processes Q50614 P9WQI9 GO:0042626,GO:0016021,GO:0005886,GO:0055085,GO:0005524 P9WQI9 NC_000962.3 Mycobrowser_v5 CDS 3710433 3710759 . + 0 Rv3325 Rv3325 Involved in the transposition of the insertion sequence element IS6110. Probable transposase for insertion sequence element IS6110 (fragment) Rv3325, (MTV016.25), len: 108 aa. Putative Transposase for IS6110 (fragment). Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv3325 and Rv3326, the sequence UUUUAAAG (directly upstream of Rv3326) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Belongs to the transposase family 8. P9WKH5 No insertion seqs and phages P0C5G9 P9WKH5 GO:0004803,GO:0006313,GO:0003677 P9WKH5 NC_000962.3 Mycobrowser_v5 CDS 2066457 2068883 . + 0 Rv1821 secA2 Involved in protein export. May interact with the SECY/SECE subunits. SECA has a central role in coupling the hydrolysis of ATP to the transfer of PRE-secretory periplasmic and outer membrane proteins across the membrane. Possible preprotein translocase ATPase SecA2 Rv1821, (MTCY1A11.22c), len: 808 aa. Possible secA2, preprotein translocase and ATPase, component of secretion apparatus (see Braunstein & Belisle 2000), similar to several preprotein translocases e.g. P28366|SECA_BACSU preprotein translocase secA subunit from Bacillus subtilis (841 aa), FASTA scores: opt: 1424, E(): 0, (35.9% identity in 786 aa overlap). Equivalent to AL008609|MLCB1788.45 Preprotein translocase SecA 2 from Mycobacterium leprae (778 aa) (87.1% identity in 780 aa overlap). Also similar to Rv3240c|MTCY20B11.15c secA preprotein translocase from Mycobacterium tuberculosis (949 aa). Could be part of the prokaryotic protein translocation apparatus which comprise SECA|Rv3240c, SECD|Rv2587c, SECE|Rv0638, SECF|Rv2586c, SECG|Rv1440 and SECY|Rv0732. Binds ATP. P9WGP3 No cell wall and cell processes P66785 P9WGP3 GO:0005737,GO:0065002,GO:0005886,GO:0005515,GO:0017038,GO:0006605,GO:0005524 P9WGP3 NC_000962.3 Mycobrowser_v5 CDS 2071952 2072356 . + 0 Rv1826 gcvH The glycine cleavage system catalyses the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein Probable glycine cleavage system H protein GcvH Rv1826, (MTCY1A11.17c), len: 134 aa. Probable gcvH, glycine cleavage system H protein, highly similar to GCSH_ECOLI|P23884 glycine cleavage system H protein from Escherichia coli (129 aa), FASTA scores: opt: 428, E(): 2.2e-22, (47.8% identity in 134 aa overlap). Equivalent to MLCB1788.37c gcvH from Mycobacterium leprae (78.4% identity in 134 aa overlap). Contains PS00189 2-oxo acid dehydrogenases acyltransferase component lipoyl binding site. Belongs to the GcvH family. P9WN55 No intermediary metabolism and respiration 3HGB,3IFT Q50607 P9WN55 GO:0019464,GO:0031405,GO:0005739,GO:0005960 P9WN55 NC_000962.3 Mycobrowser_v5 CDS 2075877 2078702 . + 0 Rv1832 gcvB The glycine cleavage system catalyses the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein [catalytic activity: glycine + lipoylprotein = S- aminomethyldihydrolipoylprotein + CO(2)] Probable glycine dehydrogenase GcvB (glycine decarboxylase) (glycine cleavage system P-protein) Rv1832, (MTCY1A11.11c), len: 941 aa. Probable gcvB, glycine dehydrogenase [decarboxylating], highly similar to GCSP_ECOLI|P33195 glycine dehydrogenase (decarboxylating) from Escherichia coli (957 aa), FASTA scores: opt: 2194, E(): 0, (55.4% identity in 961 aa overlap). The glycine cleavage system is composed of four proteins: P, T, L, and H P9WN53 No intermediary metabolism and respiration Q50601 P9WN53 GO:0004375,GO:0055114,GO:0030170,GO:0019464 1.4.4.2 P9WN53 NC_000962.3 Mycobrowser_v5 CDS 2082603 2084636 . - 0 Rv1836c Rv1836c Function unknown Conserved protein Rv1836c, (MTCY1A11.07), len: 677 aa. Conserved protein. Equivalent to MLCB1788.28|AL008609 hypothetical protein from Mycobacterium leprae (710 aa), FASTA scores: opt: 2938, E(): 0, (66.0% identity in 714 aa overlap). Contains PS00036 bZIP transcription factors basic domain signature. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WLQ9 No conserved hypotheticals P9WLQ9 GO:0005886,GO:0016021 P9WLQ9 NC_000962.3 Mycobrowser_v5 CDS 2087971 2089518 . - 0 Rv1840c PE_PGRS34 Function unknown PE-PGRS family protein PE_PGRS34 Rv1840c, (MTCY359.33), len: 515 aa. PE_PGRS34, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below). Similar to many e.g. Y03A_MYCTU|Q10637 hypothetical glycine-rich 49.6 kDa protein (603 aa), FASTA scores: opt: 1693, E(): 0, (53.1% identity in 612 aa overlap); etc. P9WIF3 No PE/PPE Q50594 P9WIF3 GO:0005886,GO:0016021 P9WIF3 NC_000962.3 Mycobrowser_v5 CDS 2090718 2092085 . - 0 Rv1842c Rv1842c Unknown Conserved hypothetical membrane protein Rv1842c, (MTCY359.31), len: 455 aa. Conserved hypothetical membrane protein. Similar to Z99109|0O7589 Potential integral membrane protein from Bacillus subtilis (461 aa), FASTA scores: opt: 723, E(): 0, (31.2% identity in 449 aa overlap). Similar to other Mycobacterium tuberculosis putative integral membrane proteins e.g. Rv2366c, Rv1841c. P9WFP3 No cell wall and cell processes Q50592 P9WFP3 GO:0005886,GO:0016021 P9WFP3 NC_000962.3 Mycobrowser_v5 CDS 2093731 2095188 . - 0 Rv1844c gnd1 Involved in hexose monophosphate shunt (pentose phosphate pathway) [catalytic activity: 6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH]. Probable 6-phosphogluconate dehydrogenase Gnd1 Rv1844c, (MTCY359.29), len: 485 aa. Probable gnd1, 6-phosphogluconate dehydrogenase. Similar to others e.g. 6PGD_ECOLI|P00350 from Escherichia coli (468 aa), FASTA scores: opt: 1661, E(): 0, (53.6% identity in 466 aa overlap); etc. Also similar to Rv1122|MTCY22G8.11|gnd2 probable 6-phosphogluconate dehydrogenase, decarboxylating from Mycobacterium tuberculosis (340 aa), FASTA score: (33.0% identity in 351 aa overlap). Note that Rv1844c is most similar to gnd's from Gram negative organisms, while Rv1122|MTCY22G8.11|gnd2 is most similar to gnd's from Gram positive organisms. Belongs to the 6-phosphogluconate dehydrogenase family. Q79FJ2 No intermediary metabolism and respiration Q79FJ2 Q79FJ2 GO:0055114,GO:0006098,GO:0004616,GO:0050661 1.1.1.44 Q79FJ2 NC_000962.3 Mycobrowser_v5 CDS 2097961 2099694 . + 0 Rv1850 ureC Involved in the conversion of urea to NH3 [catalytic activity: urea + H2O = CO2 + 2 NH3] Urease alpha subunit UreC (urea amidohydrolase) Rv1850, (MTCY359.23c), len: 577 aa. UreC, urease alpha subunit. Similar to URE1_MYCTU|P50042 from Mycobacterium tuberculosis (577 aa), FASTA scores: opt: 3794, E(): 0, (98.3% identity in 577 aa overlap). Contains PS00145 Urease active site motif. Belongs to the urease family. P9WFF1 No intermediary metabolism and respiration P0A660 P9WFF1 GO:0016151,GO:0019627,GO:0009039,GO:0005737 3.5.1.5 P9WFF1 NC_000962.3 Mycobrowser_v5 CDS 2101651 2103042 . - 0 Rv1854c ndh Transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Does not couple the redox reaction to proton translocation. Probable NADH dehydrogenase Ndh Rv1854c, (MTCY359.19), len: 463 aa. Probable ndh, NADH dehydrogenase (see citations below), similar to several e.g. S74826 NADH dehydrogenase from Synechocystis sp. (445 aa), FASTA score: opt: 1228, E(): 0, (46.3% identity in 432 aa overlap). Highly similar to Rv0392c|Z84725|g1817703 from Mycobacterium tuberculosis (470 aa), FASTA scores: opt: 1911, E(): 0, (64.7% identity in 459 aa overlap); and Rv1812c. P95160 No intermediary metabolism and respiration P95160 P95160 GO:0003954,GO:0055114,GO:0050660 1.6.99.3 INH P95160 NC_000962.3 Mycobrowser_v5 CDS 2105773 2106567 . + 0 Rv1858 modB Part of the binding-protein-dependent transport system modabc for molybdenum; responsible for the translocation of the substrate across the membrane. Probable molybdenum-transport integral membrane protein ABC transporter ModB Rv1858, (MTCY359.15c), len: 264 aa. Probable modB, molybdenum-transport integral membrane protein ABC transporter (see citation below), similar to others e.g. Y10817|ANY108175 ModB from Arthrobacter (239 aa), FASTA scores: opt: 937, E(): 0, (67.8% identity in 230 aa overlap); etc. Similar to other Mycobacterium tuberculosis transport proteins e.g. Rv2039c, Rv2316, etc. P9WG13 No cell wall and cell processes P0A624 P9WG13 GO:0015098,GO:0015689,GO:0005886,GO:0016021 P9WG13 NC_000962.3 Mycobrowser_v5 CDS 2106574 2107683 . + 0 Rv1859 modC Part of the binding-protein-dependent transport system modabc for molybdenum; responsible for energy coupling to the transport system. Probable molybdenum-transport ATP-binding protein ABC transporter ModC Rv1859, (MTCY359.14c), len: 369 aa. Probable modC, molybdenum-transport ATP-binding protein ABC transporter (see citation below), similar to others e.g. Y10817|ANY108176 ModC from Arthrobacter (349 aa), FASTA scores: opt: 895, E(): 0, (46.0% identity in 361 aa overlap); etc. Shows similarity to other Mycobacterium tuberculosis ABC-transporter proteins e.g. Rv0073, Rv1238, Rv2564, etc. Contains both PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signatures involved in molybdate uptake. Belongs to the ATP-binding transport protein family (ABC transporters). P9WQL3 No cell wall and cell processes P95155 P9WQL3 GO:0043190,GO:0030001,GO:0015689,GO:0015412,GO:0042888,GO:0005886,GO:0005524 3.6.3.29 P9WQL3 NC_000962.3 Mycobrowser_v5 CDS 3796448 3797437 . - 0 Rv3382c lytB1 Unknown. Possibly involved in drug/antibiotic tolerance. In other organisms, LYTB product is involved in penicillin tolerance and control of the stringent response. Probable LYTB-related protein LytB1 Rv3382c, (MTV004.40c), len: 329 aa. Probable lytB1, lytB-related protein, highly similar to many e.g. Q9HVM7|LYTB_PSEAE|PA4557 from Pseudomonas aeruginosa (314 aa), FASTA scores: opt: 1048, E(): 2e-55, (53.2% identity in 314 aa overlap); Q9JR39|LYTB|NMA0624|NMB1831 from Neisseria meningitidis (serogroup a and B) (322 aa), FASTA scores: opt: 1041, E(): 5.4e-55, (52.25% identity in 312 aa overlap); P22565|LYTB_ECOLI|B0029 from Escherichia coli strain K12 (316 aa), FASTA scores: opt: 1013, E(): 2.5e-53, (51.45% identity in 311 aa overlap) (for more information about lytB protein, see citation below); Q9X781|LYTB_MYCLE|LYTB2|ML1938|MLCB1222.06c from Mycobacterium leprae (332 aa), FASTA scores: opt: 979, E(): 2.8e-51, (51.3% identity in 312 aa overlap); etc. Also similar to Q9PAS9|XF2416 drug tolerance protein from Xylella fastidiosa (316 aa), FASTA scores: opt: 1043, E(): 4.1e-55, (53.65% identity in 315 aa overlap). And similar to O53458|Rv1110|LYTB2|MTV017.63 from Mycobacterium tuberculosis (335 aa), FASTA scores: opt: 975, E(): 4.9e-51, (51.3% identity in 312 aa overlap). Belongs to the LytB family. P9WKF9 No cell wall and cell processes P0A5I2 P9WKF9 GO:0051745,GO:0019288,GO:0046872,GO:0055114,GO:0016114,GO:0051538 1.17.1.2 P9WKF9 NC_000962.3 Mycobrowser_v5 CDS 2113140 2115476 . + 0 Rv1866 Rv1866 Function unknown, but supposed involvement in lipid degradation. Conserved protein Rv1866, (MTCY359.07c), len: 778 aa. Conserved protein, N-terminal region similar to fatty acyl-CoA racemases e.g. Rv0855, Rv1143, and C-terminal region (from aa 370) similar to L-carnitine dehydratases, racemases, and Rv3272|MTCY71.12 Mycobacterium tuberculosis (394 aa), FASTA score: opt: 472, E(): 2.1e-21, (29.9% identity in 388 aa overlap). Also similar to P31572|CAIB_ECOLI L-carnitine dehydratase (405 aa), FASTA score: opt: 306, E(): 2.1e-11, (23.3% identity in 424 aa overlap). P95149 No lipid metabolism P95149 P95149 GO:0008152,GO:0003824 P95149 NC_000962.3 Mycobrowser_v5 CDS 2115764 2117248 . + 0 Rv1867 Rv1867 Function unknown, but supposed involvement in lipid degradation. Conserved protein Rv1867, (MTCY359.06c), len: 494 aa. Conserved protein, some similarity to acetyl CoA synthase and to lipid carriers. FASTA best: E155295 acetyl CoA synthase (388 aa), opt: 213, E(): 4.5e-07, (23.2% identity in 423 aa overlap), P95148 No lipid metabolism P95148 GO:0008152,GO:0003824 P95148 NC_000962.3 Mycobrowser_v5 CDS 2121907 2123151 . - 0 Rv1872c lldD2 Involved in respiration; catalyzes conversion of lactate into pyruvate [catalytic activity: (S)-lactate + 2 ferricytochrome C = pyruvate + 2 ferrocytochrome C]. Possible L-lactate dehydrogenase (cytochrome) LldD2 Rv1872c, (MTCY180.46, MTCY359.01), len: 414 aa (start uncertain). Possible lldD2, L-lactate dehydrogenase (cytochrome), similar to other lactate dehydrogenases and other oxidases e.g. LLDD_ECOLI|P33232 l-lactate dehydrogenase (cytochrome) from Escherichia coli strain K12 (396 aa), FASTA results: opt: 674, E(): 1.1e-37, (40.5% identity in 279 aa overlap); Q51135 lactate dehydrogenase from Neisseria meningitidis (390 aa), FASTA results: opt: 309, E(): 4.1e-15, (42.5% identity in 113 aa overlap); etc. Also shows similarity with Rv0694|lldD1|MTCY210.11 possible L-lactate dehydrogenase (cytochrome) from Mycobacterium tuberculosis (396 aa). Contains PS00557 FMN-dependent alpha-hydroxy acid dehydrogenases active site. Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenases family. Phosphorylated in vitro by PknJ|Rv2088 (See Arora et al., 2010). P9WND5 No intermediary metabolism and respiration P95143 P9WND5 GO:0004460,GO:0055114,GO:0010181 1.1.2.3 P9WND5 NC_000962.3 Mycobrowser_v5 CDS 2179673 2180446 . + 0 Rv1927 Rv1927 Function unknown Conserved hypothetical protein Rv1927, (MTCY09F9.37c), len: 257 aa. Conserved hypothetical protein, similar to SCG11A.10c|AL133210 hypothetical protein from Streptomyces coelicolor (252 aa), FASTA scores: opt: 729, E(): 0, (48.3% identity in 238 aa overlap). Slight similarity with P54543|YQJF_BACSU hypothetical 23.9 kDa protein from Bacillus subtilis (209 aa), FASTA scores, opt: 230, E(): 2.8e-08, (28.0% identity in 164 aa overlap). P95287 No conserved hypotheticals P95287 P95287 P95287 NC_000962.3 Mycobrowser_v5 CDS 2125340 2125819 . + 0 Rv1876 bfrA Involved in iron storage (may perform analogous functions in iron detoxification and storage as that of animal ferritins); ferritin is an intracellular molecule that stores iron in a soluble, nontoxic, readily available form. The functional molecule, which is composed of 24 chains, is roughly spherical and contains a central cavity in which the polymeric ferric iron core is deposited. Probable bacterioferritin BfrA Rv1876, (MTCY180.42c), len: 159 aa. Probable bfrA (alternate gene name: bfr), bacterioferritin (see citation below), similar to BFR_MYCLE|P43315 bacterioferritin (bfr) from Mycobacterium leprae (159 aa), FASTA results: opt: 958, E(): 0, (90.6% identity in 159 aa overlap). Also similar to Rv3841|MTCY01A6.28c|bfrB possible bacterioferritin from Mycobacterium tuberculosis (181 aa). Belongs to the bacterioferritin family. P9WPQ9 No intermediary metabolism and respiration 2WTL P63697 P9WPQ9 GO:0008199,GO:0006826,GO:0055114,GO:0016491,GO:0006879 P9WPQ9 NC_000962.3 Mycobrowser_v5 CDS 2125904 2127967 . + 0 Rv1877 Rv1877 Unknown; possibly involved in transport of drug across the membrane. Probable conserved integral membrane protein Rv1877, (MTCY180.41c), len: 687 aa. Probable conserved integral membrane protein, part of major facilitator superfamily (MFS), similar to many antibiotic and drug efflux proteins. Similar to e.g. Q56175 TU22 dTDP-glucose dehydrtatase from Streptomyces violaceoruber (557 aa), FASTA scores: opt: 895, E(): 0, (34.7% identity in 528 aa overlap). Also similar to Mycobacterium tuberculosis relatives protein, include Rv3728, Rv3239c, Rv2846c, etc. Contains PS00217 Sugar transport proteins signature 2 (PS00217). P9WG85 No cell wall and cell processes O07753 P9WG85 GO:0005886,GO:0046677,GO:0015904,GO:0015520,GO:0055085,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2129377 2130513 . + 0 Rv1879 Rv1879 Function unknown Conserved hypothetical protein Rv1879, (MTCY180.39c), len: 378 aa. Conserved hypothetical protein, similar to SCC22.14c|AL096839 hypothetical protein from Streptomyces coelicolor (368 aa), FASTA results: opt: 772, E(): 0 (40.3% identity in 372 aa overlap); and to N-terminal half of nodulin/glutamate-ammonia ligase-like protein. Some similarity to N-terminus of AL132958|ATT4D2_11 Arabidopsis thaliana (845 aa), FASTA results: opt: 354, E(): 3.1e-16, (29.2% identity in 383 aa overlap); and to P38094|FLUG_EMENI Flug protein of Emericella nidulans (865 aa), FASTA results: opt: 306, E(): 6.2e-13, (26.5% identity in 415 aa overlap). Note that the upstream ORF Rv1878|MTCY18 0.40c is similar to the C-terminus. O07751 No conserved hypotheticals O07751 O07751 GO:0008152,GO:0003824 O07751 NC_000962.3 Mycobrowser_v5 CDS 2130541 2131857 . - 0 Rv1880c cyp140 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Probable cytochrome P450 140 Cyp140 Rv1880c, (MT1929, MTCY180.38), len: 438 aa. Probable cyp140, cytochrome p450. Similar to Q00441|CPXJ_SACER 6-deoxyerythronolide beta hydroxylase (404 aa), FASTA scores: opt: 775, E(): 0, (44.2% identity in 319 aa overlap); and other members of the cytochrome P450 family. Related to Mycobacterium tuberculosis proteins include: Rv0766c, Rv2266, Rv0778, etc. Contains cytochrome P450 cysteine heme-iron ligand signature (PS00086). Belongs to the cytochrome P450 family. P9WPL9 No intermediary metabolism and respiration P63721 P9WPL9 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPL9 NC_000962.3 Mycobrowser_v5 CDS 2132370 2133203 . - 0 Rv1882c Rv1882c Function unknown; probably involved in cellular metabolism Probable short-chain type dehydrogenase/reductase Rv1882c, (MTCY180.36), len: 277 aa. Probable short-chain dehydrogenase/reductase, similar to various dehydrogenases/reductases, generally belonging to SDR family, e.g. NP_250789.1|NC_002516 probable short-chain dehydrogenase from Pseudomonas aeruginosa (251 aa); NP_421760.1|NC_002696 short chain dehydrogenase family protein from Caulobacter crescentus (270 aa); NP_107167.1|NC_002678 oxidoreductase (short chain dehydrogenase/reductase family) from Mesorhizobium loti (253 aa); P50197|LINC_PSEPA 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase from Pseudomonas paucimobilis (Sphingomonas paucimobilis) (250 aa), FASTA scores: opt: 301, E(): 2.3e-12, (30.0% identity in 223 aa overlap); etc. Also similar to proteins from Mycobacterium tuberculosis e.g. Rv3057c, Rv1245, etc. Contains possible helix-turn-helix motif at aa 246-267 (+4.32 SD). Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. O07749 No intermediary metabolism and respiration O07749 O07749 GO:0055114,GO:0016491 1.1.-.- O07749 NC_000962.3 Mycobrowser_v5 CDS 2134890 2135867 . - 0 Rv1886c fbpB Involved in cell wall mycoloylation. Proteins of the antigen 85 complex are responsible for the high affinity of mycobacteria to fibronectin. Possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity. Secreted antigen 85-B FbpB (85B) (antigen 85 complex B) (mycolyl transferase 85B) (fibronectin-binding protein B) (extracellular alpha-antigen) Rv1886c, (MT1934, MTCY180.32), len: 325 aa. FbpB (alternate gene names: mpt59, 85B), precursor of the 85-B antigen (fibronectin-binding protein B) (mycolyl transferase 85B) (see citations below), highly similar to other Mycobacterial antigen precursors e.g. P12942|A85B_MYCBO antigen 85-B precursor from Mycobacterium bovis (323 aa); P21160|A85B_MYCKA antigen 85-B precursor from Mycobacterium kansasii (325 aa); etc. Also highly similar to Mycobacterium tuberculosis antigen precursors: Rv3804c|fbpA (338 aa), Rv0129c|fbpC2 (340 aa), and Rv3803c|fbpC1 (299 aa). Predicted possible vaccine candidate (See Zvi et al., 2008). P9WQP1 No lipid metabolism 1F0N,1F0P P0C5B9 P9WQP1 GO:0005576,GO:0008415 2.3.1.- P9WQP1 NC_000962.3 Mycobrowser_v5 CDS 2194644 2194973 . - 0 Rv1942c mazF5 Function unknown Possible toxin MazF5 Rv1942c, (MTCY09F9.22), len: 109 aa. Possible mazF5, toxin, part of toxin-antitoxin (TA) operon with Rv1943c (See Pandey and Gerdes, 2005; Zhu et al., 2006), shows some similarity to Q10867|MTCY39.28|Rv1991 hypothetical 12.3 kDa protein (114 aa), FASTA scores: opt: 117, E(): 0.021, (24. 5% identity in 110 aa overlap) also P33645|CHPA_ECOLI pemk-like protein 1 (mazf protein) from Escherichia coli (111 aa), FASTA scores: opt: 104, E(): 0.18, (29.1% identity in 110 aa overlap). Also similar to Mycobacterium tuberculosis Rv0659c (102 aa) (32.7% identity in 101 aa overlap); Rv1102c (33.3% identity in 93 aa overlap) and Rv1495. P95272 No virulence, detoxification, adaptation P95272 P95272 GO:0003677 P95272 NC_000962.3 Mycobrowser_v5 CDS 2140739 2141869 . - 0 Rv1894c Rv1894c Function unknown Conserved hypothetical protein Rv1894c, (MTCY180.24), len: 376 aa. Conserved hypothetical protein, weak similarity to some oxidoreductases e.g. Q01284 2-nitropropane dioxygenase precursor (378 aa), FASTA results: opt: 204, E(): 5.8e-06, (34.3% identity in 140 aa overlap). Similar to hypothetical Mycobacterium tuberculosis proteins e.g. Rv3553|MTCY03C7.02c (355 aa), FASTA results: opt: 296, E(): 1.6e-10, (32.9% identity in 167 aa overlap); Rv1533 (375 aa) (48.1% identity in 376 aa overlap); Rv0021c, Rv2781c. O07738 No conserved hypotheticals O07738 O07738 GO:0055114,GO:0018580 O07738 NC_000962.3 Mycobrowser_v5 CDS 2146245 2147633 . - 0 Rv1900c lipJ Function unknown; probably involved in cellular metabolism Probable lignin peroxidase LipJ Rv1900c, (MTCY180.18), len: 462 aa. Probable lipJ, lignin peroxidase, with some similarity to esterases, hydrolases and hypothetical Mycobacterium tuberculosis proteins e.g. Q43936 beta-ketoadipate enol-lactone hydrolase from Acinetobacter calcoaceticus (267 aa), FASTA results: opt: 217, E(): 1.7e-07, (29.2% identity in 260 aa overlap). Also similar to other Mycobacterium tuberculosis hypothetical proteins e.g. Rv2212|Q10400|YM12_MYCTU (378 aa), FASTA results: opt: 216, E(): 6.7e-07, (27.7% identity in 285 aa overlap). O07732 No intermediary metabolism and respiration 1YBT,1YBU O07732 O07732 GO:0004601,GO:0016849,GO:0009190 O07732 NC_000962.3 Mycobrowser_v5 CDS 2153235 2153882 . - 0 Rv1907c Rv1907c Unknown Hypothetical protein Rv1907c, (MTCY180.11), len: 215 aa. Hypothetical unknown protein. Similar to Q50763 Ethyl methane sulphonate resistance protein from Mycobacterium tuberculosis (168 aa), FASTA scores: opt: 638, E(): 0, (69.7% identity in 152 aa overlap). Downstream of a cloned katG gene (EMBL:mtkatg). Differences are due to frameshift errors in the EMBL sequence and the use of an earlier start codon. Alternative nucleotide at position 2153410 (a->G; V158A) has been observed. L0TAY1 No conserved hypotheticals L0TAY1 NC_000962.3 Mycobrowser_v5 CDS 2153889 2156111 . - 0 Rv1908c katG Multifunctional enzyme, exhibiting both a catalase, a broad-spectrum peroxidase, and a peroxynitritase activities. May play a role in the intracellular survival of mycobacteria within macrophages; protection against reactive oxygen and nitrogen intermediates produced by phagocytic cells. Seems regulated by SIGB|Rv2710 [catalytic activity: 2 H(2)O(2) = O(2) + 2 H(2)O]. Catalase-peroxidase-peroxynitritase T KatG Rv1908c, (MTCY180.10), len: 740 aa. KatG, catalase-peroxidase-peroxynitritase T (see citations below), HPI. FASTA results: Q57215 catalase-peroxidase from Mycobacterium tuberculosis (740 aa) opt: 5081, E(): 0, (100% identity in 740 aa overlap). Contains peroxidases active site signature (PS00436) and ATP/GTP-binding site motif A (P-loop; PS00017). Cosmid sequence was corrected to agree with a sequencing read from the H37Rv genome. Deletions or defects in KATG gene cause isoniazid (INH) resistance. Belongs to the peroxidase family. Bacterial peroxidase/catalase subfamily. KATG transcription seems to be regulated by FURA|Rv1909c product. The catalase-peroxidase activity is associated with the amino-terminal domain but no definite function has been assigned to the carboxy-terminal domain. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WIE5 No virulence, detoxification, adaptation 1SFZ,1SJ2,2CCA,2CCD Q08129 P9WIE5 GO:0020037,GO:0042744,GO:0055114,GO:0046677,GO:0004096 1.11.1.7,1.11.1.6 INH P9WIE5 NC_000962.3 Mycobrowser_v5 CDS 2158087 2159091 . - 0 Rv1912c fadB5 Thought to be involved in fatty acid degradation. FADB and FADA are the alpha and beta subunits of the multifunctional enzyme complex of the fatty acid degradation cycle. Possible oxidoreductase FadB5 Rv1912c, (MTCY180.06), len: 334 aa. Possible fadB5, oxidoreductase, similar to various oxidoreductases: 3-hydroxyacyl-CoA dehydrogenase, quinone oxidoreductases, and polyketide synthases, e.g. NP_104067.1|NC_002678 probable oxidoreductase from Mesorhizobium loti (308 aa); NP_464140.1|NC_003210 protein similar to oxidoreductase from Listeria monocytogenes (313 aa); NP_193889.1|NC_003075 putative NADPH quinone oxidoreductase from Arabidopsis thaliana (325 aa); NP_001880.2|NM_001889 crystallin, zeta; quinone oxidoreductase; NADPH:quinone reductase from Homo sapiens (329 aa); part 2983 to 3197 of T17410 polyketide synthase type I from Streptomyces venezuelae (3739 aa); Q53927|SCBAC20F6.16 hydroxyacyl-CoA dehydrogenase from Streptomyces coelicolor (329 aa), FASTA scores: opt: 621, E(): 2e-30, (39.5% identity in 349 aa overlap); etc. Also similar to many hypothetical Mycobacterium tuberculosis proteins including: MTCY24G1.09, MTCY13D12.11, MTCY19H9.01, MTCY24G1.03, MTCY03A2.17c, etc. Contains quinone oxidoreductase/zeta-crystallin signature (PS01162). O07721 No lipid metabolism O07721 O07721 GO:0055114,GO:0008270,GO:0003960 1.6.5.5 O07721 NC_000962.3 Mycobrowser_v5 CDS 2161566 2162762 . + 0 Rv1916 aceAb Involved in glyoxylate bypass, an alternative to the tricarboxylic acid cycle [catalytic activity: isocitrate = succinate + glyoxylate]. Probable isocitrate lyase AceAb [second part] (isocitrase) (isocitratase) (Icl) Rv1916, (MTCY180.02c), len: 398 aa. Probable aceAb, isocitrate lyase (see citations below). Highly similar to the C-terminus of ACEA_MYCLE|P46831 isocitrate lyase from Mycobacterium leprae (606 aa), FASTA results: opt: 1635, E(): 0, (86.3% identity in 278 aa overlap). Although this ORF and the upstream ORF representing the N-terminal half of aceA could be joined by a frameshift no error is apparent in the cosmid, or in a seqencing read from the genome of H37Rv. As this ORF has a RBS and transcriptional start immediately following the stop of the upstream ORF, it is possible that they are expressed as two separate modules. In Mycobacterium tuberculosis strain CDC1551, aceA exists as a single gene, MT1966: the corresponding protein has been purified experimentally and seems have an active isocitrate lyase activity (see Honer et al., 1999). For Mycobacterium tuberculosis strain H37Rv, immunoblot assay didn't detect AceAa or AceAb products (see Honer et al., 1999) but mRNA of AceAa|Rv1915 has been detected (see Betts et al., 2002); so AceAb|Rv1916 could be a pseudogene. Icl2 has 2-methyl-isocitrate lyase (MCL) activity in M. tuberculosis Erdman (See Munoz-Elias et al., 2006). O07717 No intermediary metabolism and respiration O07717 O07717 GO:0004451,GO:0019752 4.1.3.1 O07717 NC_000962.3 Mycobrowser_v5 CDS 2171623 2172486 . + 0 Rv1920 Rv1920 Unknown Probable membrane protein Rv1920, (MTV050.04), len: 287 aa. Probable membrane protein, similar to AL0215|SC10A5.04 putative membrane protein from Streptomyces coelicolor cosmid 10A5 (295 aa), FASTA scores: opt: 292, E(): 3.6e-13, (31.3% identity in 243 aa overlap). Also weakly similar to several Mycobacterial putative proteins with unknown function e.g. Rv0502, Rv1428c, U00018_22 Mycobacterium leprae cosmid B2168. O53962 No cell wall and cell processes O53962 O53962 GO:0008152,GO:0008415 O53962 NC_000962.3 Mycobrowser_v5 CDS 2174067 2175182 . + 0 Rv1922 Rv1922 Unknown Probable conserved lipoprotein Rv1922, (MTCY09F9.42c), len: 371 aa. Probable conserved lipoprotein, possibly peptidase similar to many peptidases, e.g. P15555|DAC_STRSQ D-alanyl-D-alanine carboxypeptidase from Streptomyces sp. (406 aa), FASTA scores: opt: 382, E(): 3.1e-17, (28.0% identity in 379 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins Rv1497, Rv2463, Rv3775, etc. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. P95291 No cell wall and cell processes P95291 P95291 P95291 NC_000962.3 Mycobrowser_v5 CDS 2177087 2178949 . + 0 Rv1925 fadD31 Function unknown, but involvement in lipid degradation. Probable acyl-CoA ligase FadD31 (acyl-CoA synthetase) (acyl-CoA synthase) Rv1925, (MTCY09F9.39c), len: 620 aa. Probable fadD31, acyl-CoA synthetase, highly similar to others from Mycobacterium leprae e.g. NP_301198.1|NC_002677 putative acyl-CoA synthetase (635 aa); NP_302537.1|NC_002677 probable acyl-CoA synthase (583 aa); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. fadD32 (637 aa); fadD21 (578 aa); fadD29 (619 aa); fadD26|FD26_MYCTU|Q10976 (626 aa), FASTA scores: opt: 945, E(): 0, (39.8% identity in 598 aa overlap); etc. Also similar to N-terminus of G1171128 saframycin MX1 synthetase B from Myxococcus xanthus (1770 aa), FASTA scores: opt: 845, E(): 0, (37.4% identity in 593 aa overlap); N-terminus of T34918 polyketide synthase from Streptomyces coelicolor (2297 aa); etc. Nucleotide position 2177654 in the genome sequence has been corrected, A:C resulting in M190L. I6Y7V6 No lipid metabolism P95288 I6Y7V6 GO:0008152,GO:0016874 6.2.1.- I6Y7V6 NC_000962.3 Mycobrowser_v5 CDS 2180450 2181217 . - 0 Rv1928c Rv1928c Function unknown; probably involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv1928c, (MTCY09F9.36), len: 255 aa. Probable short-chain dehydrogenase/reductase, highly similar to others e.g. NP_228109.1|NC_000853 oxidoreductase (short chain dehydrogenase/reductase family) from Thermotoga maritima (257 aa); T41116 short chain dehydrogenase from Schizosaccharomyces pombe (261 aa); P87219|SOU1_CANAL sorbitol utilization protein (SDR family) from Candida albicans (281 aa); P25529|HDHA_ECOLI 7-alpha-hydroxysteroid dehydrogenase from Escherichia coli (255 aa), FASTA scores: opt: 541, E(): 1.2e-27, (37.5% identity in 251 aa overlap); etc. Also similar to many mycobacterial tuberculosis proteins e.g. Rv1350, Rv0927c, Rv2002, Rv0769, Rv2766c, etc. Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. P95286 No intermediary metabolism and respiration P95286 P95286 GO:0055114,GO:0016491 1.-.-.- P95286 NC_000962.3 Mycobrowser_v5 CDS 2184959 2186188 . - 0 Rv1934c fadE17 Function unknown, but supposed involvement in lipid degradation. Probable acyl-CoA dehydrogenase FadE17 Rv1934c, (MTCY09F9.30), len: 409 aa. Probable fadE17, acyl-CoA dehydrogenase, highly similar to ACD_MYCLE|P46703 acyl-CoA dehydrogenase from Mycobacterium leprae (389 aa), FASTA scores: opt: 414, E(): 2.6e-19, (28.3% identity in 407 aa overlap). Also similar to many e.g. NP_249713.1|NC_002516 probable acyl-CoA dehydrogenase from Pseudomonas aeruginosa (381 aa); NP_420614.1|NC_002696 acyl-CoA dehydrogenase family protein from Caulobacter crescentus (355 aa); CAB61610.1|AL133210 putative acyl-CoA dehydrogenase from Streptomyces coelicolor (393 aa); etc. Also similar to others from Mycobacterium tuberculosis e.g. fadE30 (385 aa); fadE31 (377 aa); C-terminus of fadE34 (711 aa); etc. Could belong to the acyl-CoA dehydrogenases family. P95280 No lipid metabolism P95280 P95280 GO:0050660,GO:0003995,GO:0055114 1.3.99.- P95280 NC_000962.3 Mycobrowser_v5 CDS 2188496 2191015 . + 0 Rv1937 Rv1937 Function unknown; may be involved in electron transfer. Possible oxygenase Rv1937, (MTCY09F9.27c), len: 839 aa. Possible oxygenase, similar in N-terminus to N-terminal part (approx. 350 aa) of dioxygenases (including ring-hydroxylating dioxygenase electron transfer components) and monooxygenases, e.g. AAC34815.1|AF071556 anthranilate dioxygenase reductase from Acinetobacter sp. (343 aa); AAK52291.1|AY026914|AntC putative anthranilate dioxygenase reductase from Pseudomonas putida (340 aa); AAF63450.1|AF218267_7|AF218267 benzoate dioxygenase / ferredoxin reductase from Pseudomonas putida (336 aa); P23101|XYLZ_PSEPU toluate 1,2-dioxygenase electron transfer component [includes: ferredoxin; ferredoxin--NAD(+) reductase ] from Pseudomonas putida plasmid TOL pWW0 (336 aa), FASTA scores: opt: 700, E(): 0, (34.3% identity in 335 aa overlap); S23479 probable benzoate 1,2-dioxygenase reductase component benC from Acinetobacter calcoaceticus (338 aa); AAC45294.1|U81594 soluble methane monooxygenase protein C from Methylocystis sp. (343 aa); P22868|MEMC_METCA methane monooxygenase component C from Methylococcus capsulatus (348 aa); etc. Also similar in part to Mycobacterium tuberculosis hypothetical electron transfer proteins Rv3554, Rv3571, etc. Contains PS00197 2Fe-2S ferredoxins, iron-sulfur binding region signature. P95277 No intermediary metabolism and respiration P95277 P95277 GO:0009055,GO:0046872,GO:0055114,GO:0016491,GO:0051537 1.-.-.- P95277 NC_000962.3 Mycobrowser_v5 CDS 2192094 2192609 . + 0 Rv1939 Rv1939 Function unknown; probably involved in cellular metabolism Probable oxidoreductase Rv1939, (MTCY09F9.25c), len: 171 aa. Probable oxidoreductase, similar to NP_302637.1|NC_002677 probable oxidoreductase from Mycobacterium leprae (162 aa) Also similar to NTAB_CHELE|P54990 nitrilotriacetate monooxygenase component from Chelatobacter heintzii (322 aa), fasta scores: opt: 269, E(): 5.3e-11, (33.1% identity in 151 aa overlap). And similar to Mycobacterium tuberculosis probable monooxygenase components Rv0246, Rv3567, and to a lesser extent, Rv3007c. P95275 No intermediary metabolism and respiration P95275 P95275 GO:0055114,GO:0016491,GO:0010181 1.-.-.- P95275 NC_000962.3 Mycobrowser_v5 CDS 2202138 2202587 . + 0 Rv1956 higA Possibly involved in transcriptional mechanism. Possible antitoxin HigA Rv1956, (MTCY09F9.08c), len: 149 aa. Possible higA, antitoxin, part of toxin-antitoxin (TA) operon with Rv1955 (See Pandey and Gerdes, 2005; Gupta, 2009). Possible transcriptional regulatory protein, contains probable helix-turn-helix motif at aa 52-73 (+4.78 SD). Upon expression in E.coli Rv1956 has been shown to function as a toxin inhibiting cell growth and colony formation that is neutralized by coexpression with Rv1955 (PubMed: 19016878); It is not clear if these conflicting results are due to expression in a heterologous system. The gene names higA and higB have been assigned to both Rv1955 and Rv1956; we have chosen to call Rv1956 higA after consulting the authors. P9WJA7 No virulence, detoxification, adaptation P95258 P9WJA7 GO:0043565 P9WJA7 NC_000962.3 Mycobrowser_v5 CDS 2930344 2930784 . + 0 Rv2602 vapC41 Unknown Possible toxin VapC41. Contains PIN domain. Rv2602, (MTCI270A.03c), len: 146 aa. Possible vapC41, toxin, part of toxin-antitoxin (TA) operon with Rv2601A, contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis (strains H37Rv and CDC1551) e.g. O50457|Rv1242|MTV006.14 (143 aa), FASTA scores: opt: 147, E(): 0.0021, (26.25% identity in 141 aa overlap); P95023|Rv2530c|MTCY159.26 (139 aa), FASTA scores: opt: 131, E(): 0.027, (33.35% identity in 135 aa overlap); O53812|Rv0749|MTV041.23 (142 aa), FASTA scores: opt: 125, E(): 0.072, (26.45% identity in 140 aa overlap). P9WF59 No virulence, detoxification, adaptation O33215 P9WF59 P9WF59 NC_000962.3 Mycobrowser_v5 CDS 2205582 2206802 . - 0 Rv1963c mce3R Involved in transcriptional mechanism; repression of the MCE3 operon. Could also have a regulatory action on the MCE2 operon. Probable transcriptional repressor (probably TetR-family) Mce3R Rv1963c, (MTV051.01c-MTCY09F9.01), len: 406 aa. Probable mce3R, negative transcriptional regulatory protein, TetR family (see citation below); similar to several transcriptional regulator e.g. AL049485|SC6A5.30 Streptomyces coelicolor cosmid 6 a (404 aa), FASTA scores: opt: 319, E(): 6.4e-13, (29.5% identity in 373 aa overlap); and Z84498|MTCY9F9_1 (259 aa), FASTA scores: opt: 208, E(): 1.6e-07, (100.0% identity in 32 aa overlap). Contains probable helix-turn-helix at aa 36-57 (+4.23 SD) and two tet-R family signatures. P95251 No regulatory proteins P95251 P95251 GO:0006350,GO:0003700,GO:0006355 P95251 NC_000962.3 Mycobrowser_v5 CDS 2208507 2209322 . + 0 Rv1965 yrbE3B Unknown Conserved hypothetical integral membrane protein YrbE3B Rv1965, (MTV051.03), len: 271 aa. YrbE4B, hypothetical unknown integral membrane protein, part of mce3 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07413|Rv0168|MTCI28.08|yrbE1B (289 aa), FASTA scores: opt: 937, E(): 0, (54.3% identity in 254 aa overlap); O07790|Rv0588|MTCY19H5.34|yrbE2B (295 aa); etc. Also highly similar to conserved hypothetical integral membrane proteins of the yrbEB type, e.g. AAD24545.1|AF116213|YrbE1B from Mycobacterium leprae (106 aa); P45392|YRBE_ECOLI hypothetical 27.9 kDa protein from Escherichia coli (260 aa), FASTA scores: opt: 218, E(): 1.2e-07, (24.1% identity in 245 aa overlap); etc. The transcription of this CDS seems negatively regulated by the product of Rv1963c|mce3R (see Santangelo et al., 2002). O53966 No virulence, detoxification, adaptation O53966 O53966 O53966 NC_000962.3 Mycobrowser_v5 CDS 2209327 2210604 . + 0 Rv1966 mce3A Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce3A Rv1966, (MTV051.04), len: 425 aa. Mce3A; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins P72013|MCE1|Rv0169|MTCI28.09|mce1A (454 aa); O07789|MCE2|Rv0589|MTCY19H5.33c|mce2A (404 aa); etc. Also highly similar to others e.g. AAD52105.1|AF113402_1|AF113402 mycobacterial cell entry protein from Mycobacterium bovis BCG (454 aa); NP_302656.1|NC_002677 putative cell invasion protein from Mycobacterium leprae (441 aa); CAC12798.1|AL445327 putative secreted protein from Streptomyces coelicolor (418 aa); etc. Contains a possible N-terminal signal sequence or membrane anchor. Note that previously known as mce3. The transcription of this CDS seems negatively regulated by the product of Rv1963c|mce3R (see Santangelo et al., 2002). Predicted to be an outer membrane protein (See Song et al., 2008). L7N698 No virulence, detoxification, adaptation Q7D7P0 L7N698 L7N698 NC_000962.3 Mycobrowser_v5 CDS 2211626 2212858 . + 0 Rv1968 mce3C Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce3C Rv1968, (MTV051.06), len: 410 aa. Mce3C; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07415|R0171|MTCI28.11|mce1C (515 aa); O07787|Rv0591|MTCY19H5.31|mce2C (481 aa); etc. Also similar to others e.g. CAC12796.1|AL445327 putative secreted protein from Streptomyces coelicolor (351 aa); NP_302658.1|NC_002677 putative secreted protein from Mycobacterium leprae (519 aa); etc. Contains a possible N-terminal signal sequence or membrane anchor. The transcription of this CDS seems negatively regulated by the product of Rv1963c|mce3R (see Santangelo et al., 2002). Predicted to be an outer membrane protein (See Song et al., 2008). O53969 No virulence, detoxification, adaptation O53969 O53969 O53969 NC_000962.3 Mycobrowser_v5 CDS 2219754 2220800 . + 0 Rv1977 Rv1977 Function unknown Conserved protein Rv1977, (MTV051.15), len: 348 aa. Conserved protein, similar to SCC123.20|AL136518 hypothetical protein from Streptomyces coelicolor (402 aa), blastp scores: Score = 311 bits (789), Expect = 5e-84 Identities = 156/316 (49%), Positives = 212/316 (66%); and PCC6803|D90907_31 Synechocystis sp. (303 aa), FASTA scores: opt: 533, E(): 4.7e- 29, (38.5% identity in 275 aa overlap). Contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. Alternative nucleotide at position 2219929 (T->C; L59P) has been observed. O53978 No conserved hypotheticals O53978 O53978 GO:0046872,GO:0004222,GO:0006508,GO:0016021 O53978 NC_000962.3 Mycobrowser_v5 CDS 2234991 2237306 . - 0 Rv1992c ctpG Metal cation-transporting ATPase; possibly catalyzes the transport of an undetermined metal cation with the hydrolysis of ATP [catalytic activity: ATP + H(2)O + undetermined metal cation(in) = ADP + phosphate + undetermined metal cation(out)]. Probable metal cation transporter P-type ATPase G CtpG Rv1992c, (MTCY39.27), len: 771 aa. Probable ctpG, metal cation-transporting P-type ATPase G (transmembrane protein), similar to others, especially cadmium-transporting ATPases, e.g. NP_244904.1|NC_002570 cadmium-transporting ATPase from Bacillus halodurans (707 aa); P30336|CADA_BACFI probable cadmium-transporting ATPase from Bacillus firmus (723 aa); BAB47609.1|AB037671 cadmium resistance protein B from Staphylococcus aureus (804 aa); 3121832|Q60048|CADA_LISMO probable cadmium-transporting ATPase from Listeria monocytogenes (707 aa); etc. Also similar to others from Mycobacterium tuberculosis e.g. Rv0969|MTCY10D7.05c|ctpV putative cation transporter P-type ATPase V (770 aa); Rv1469; Rv0092; etc. Contains PS00435 Peroxidases proximal heme-ligand signature and PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases), subfamily IB. P9WPS7 No cell wall and cell processes P63689 P9WPS7 GO:0006754,GO:0015662,GO:0016021,GO:0046872,GO:0046873,GO:0030001,GO:0005886,GO:0005524 3.6.3.- P9WPS7 NC_000962.3 Mycobrowser_v5 CDS 2224220 2225188 . - 0 Rv1981c nrdF1 Involved in the DNA replication pathway. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides, precursors that are necessary for DNA synthesis [catalytic activity: 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H(2)O = ribonucleoside diphosphate + reduced thioredoxin]. Ribonucleoside-diphosphate reductase (beta chain) NrdF1 (ribonucleotide reductase small subunit) (R2F protein) Rv1981c, (MTCY39.38), len: 322 aa. NrdF1, ribonucleoside-diphosphate reductase, beta chain (see citation below), highly similar to others e.g. RIR4_SALTY|P17424 ribonucleoside-diphosphate reductase (319 aa), FASTA scores: opt: 1402, E(): 0, (66.0% identity in 315 aa overlap); etc. Also similar to Rv3048c|MTV012.63c from Mycobacterium tuberculosis. Contains PS00368 Ribonucleotide reductase small subunit signature. Belongs to the ribonucleoside diphosphate reductase small chain family. Cofactor: binds 2 iron ions (by similarity). Note that previously known as nrdF. P9WH73 No information pathways Q10840 P9WH73 GO:0009186,GO:0055114,GO:0004748,GO:0046914,GO:0006260 1.17.4.1 P9WH73 NC_000962.3 Mycobrowser_v5 CDS 2226244 2227920 . + 0 Rv1983 PE_PGRS35 Function unknown PE-PGRS family protein PE_PGRS35 Rv1983, (MTCY39.36c), len: 558 aa. PE_PGRS35, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002). Similar to other PE proteins e.g. Rv0977, etc. Contains PS00141 Eukaryotic and viral aspartyl proteases active site. P9WIF1 No PE/PPE Q10873 P9WIF1 GO:0005886,GO:0016021 P9WIF1 NC_000962.3 Mycobrowser_v5 CDS 2243816 2245138 . - 0 Rv1999c Rv1999c Unknown. Possibly transporter involved in transport of undetermined substrate (possibly cationic amino acids) across the membrane: so responsible for the translocation of the substrate across the membrane. Probable conserved integral membrane protein Rv1999c, (MTCY39.19), len: 440 aa. Probable conserved integral membrane protein, possibly transporter of cationic amino acid, similar to many transporters, especially amino acid transporters, e.g. CAC08265.1|AL392146 putative amino acid transporter from Streptomyces coelicolor (414 aa); P39277|YJEH_ECOLI hypothetical 44.8 kDa protein from Escherichia coli (418 aa), FASTA scores, opt: 343, E(): 6.6e-15, (27.2% identity in 408 aa overlap); etc. Also similar to Rv1979c from Mycobacterium tuberculosis, FASTA score: (28.2% identity in 277 aa overlap); Rv2127, Rv0346c, Rv0522, etc. Seems to belong to the APC family. P9WQM3 No cell wall and cell processes P63349 P9WQM3 GO:0006865,GO:0016021,GO:0005886,GO:0055085,GO:0015171 P9WQM3 NC_000962.3 Mycobrowser_v5 CDS 2263998 2265038 . + 0 Rv2017 Rv2017 Thought to be involved in transcriptional mechanism. Transcriptional regulatory protein Rv2017, (MTV018.04), len: 346 aa. Transcriptional regulator. Contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature in C-terminal half, may be fortuitous. Contains probable helix-turn-helix motif at aa 18-39 (Score 2243, +6.83 SD); IPR001387 Helix-turn-helix type 3. O53463 No regulatory proteins O53463 O53463 GO:0043565 O53463 NC_000962.3 Mycobrowser_v5 CDS 2268693 2270240 . - 0 Rv2024c Rv2024c Unknown Conserved hypothetical protein Rv2024c, (MTV018.11c), len: 515 aa. Conserved hypothetical protein. Identical to N-terminal part of much larger hypothetical protein, RvD1-Rv2024c' (1606 aa), from Mycobacterium bovis BCG: CAB44655.1|Y18605|13881753|AAK46361.1|AE007059 so probably truncated. Part of RvD1 chromosomal deletion region. O53470 No conserved hypotheticals O53470 O53470 GO:0003677,GO:0009307,GO:0004519,GO:0005524 O53470 NC_000962.3 Mycobrowser_v5 CDS 2272787 2274508 . - 0 Rv2027c dosT Sensor part of the two component regulatory system DEVR/DEVS/dost; O2, NO and CO are ligands, dost is inactive when bound to oxygen Two component sensor histidine kinase DosT Rv2027c, (MTV018.14c), len: 573 aa. DosT, Histidine kinase response regulator, highly similar to others. P9WGK1 No regulatory proteins 2VZW,3ZXQ O53473 P9WGK1 GO:0005737,GO:0016021,GO:0046872,GO:0046983,GO:0000155,GO:0000160,GO:0005524 P9WGK1 NC_000962.3 Mycobrowser_v5 CDS 2289282 2289599 . - 0 Rv2044c Rv2044c Unknown Conserved hypothetical protein Rv2044c, (MTV018.31c), len: 105 aa. Conserved hypothetical protein, similar to many. O53487 No conserved hypotheticals O53487 O53487 NC_000962.3 Mycobrowser_v5 CDS 3871084 3872496 . - 0 Rv3450c eccB4 Unknown ESX conserved component EccB4. ESX-4 type VII secretion system protein. Probable membrane protein. Rv3450c, (MTCY13E12.03c), len: 470 aa. EccB4, esx conserved component, ESX-4 type VII secretion system protein, probable membrane protein (possible membrane spanning region near N-terminus). Similar to hypothetical unknowns proteins from Mycobacterium leprae e.g. O33088|MLCB628.17C|ML0054 hypothetical 51.9 KDA protein (putative membrane protein)(481 aa), FASTA scores: opt: 708, E(): 6.4e-32, (32.9% identity in 480 aa overlap); Q9CD29|ML2536 (552 aa), FASTA scores: opt: 394, E(): 1.7e-14, (33.6% identity in 503 aa overlap); etc. Also similar to other proteins from Mycobacterium tuberculosis (strains H37Rv and CDC1551) e.g. O69734|Rv3869|MTV027.04 (480 aa), FASTA scores: opt: 717, E(): 2e-32, (32.55% identity in 479 aa overlap); O05449|Rv3895c|MTCY15F10.17 (495 aa), FASTA scores: opt: 670, E(): 8.3e-30, (36.4% identity in 475 aa overlap); O5368|Rv0283|MTV035.11 (538 aa), FASTA scores: opt: 467, E(): 1.5e-18, (36.3% identity in 493 aa overlap); etc. P9WNR1 No cell wall and cell processes O06317 P9WNR1 GO:0005886,GO:0016021 P9WNR1 NC_000962.3 Mycobrowser_v5 CDS 2279129 2280124 . + 0 Rv2032 acg Unknown. May have a role for bacteria within the host environment. Conserved protein Acg Rv2032, (MTV018.19), len: 331 aa. Acg (for acr-coregulated gene), conserved protein possibly member of a superfamily of classical nitroreductases (see Purkayastha et al., 2002), similar to Rv3127 and Rv3131. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WIZ9 No conserved hypotheticals O53476 P9WIZ9 GO:0016491,GO:0055114 1.-.-.- P9WIZ9 NC_000962.3 Mycobrowser_v5 CDS 2308131 2310755 . - 0 Rv2051c ppm1 Transfers mannose from GDP-mannose to all endogenous polyprenol-phosphates. Polyprenol-monophosphomannose synthase Ppm1 Rv2051c, (MTV018.38c), len: 874 aa. Ppm1, Polyprenol-monophosphomannose synthase. Transfers mannose from GDP-Mannose to all endogenous polyprenol-phosphates in Mycobacterium tuberculosis, proven experimentally (A. Baulard, Institut Pasteur de Lille: see citation below). O53493 No cell wall and cell processes O53493 O53493 GO:0016810,GO:0016021,GO:0042158,GO:0006807,GO:0005886,GO:0016410 O53493 NC_000962.3 Mycobrowser_v5 CDS 2317169 2320753 . - 0 Rv2062c cobN Required for cobalt insertion. Cobalamin biosynthesis protein CobN Rv2062c, (MTCY49.01c, MTV019.03), len: 1194 aa. cobN, cobalamin biosynthesis protein, similar to many. O53498 No intermediary metabolism and respiration O53498 O53498 GO:0009058 O53498 NC_000962.3 Mycobrowser_v5 CDS 2323175 2324701 . + 0 Rv2066 cobI Involved in cobalamin biosynthesis. Probable bifunctional protein, CobI-COBJ fusion protein: S-adenosyl-L-methionine-precorrin-2 methyl transferase + precorrin-3 methylase Rv2066, (MTCY49.05), len: 508 aa. Probable CobI-CobJ fusion protein, S-adenosyl-L-methionine-precorrin-2 methyl transferase and precorrin-3 methylase. Similar in N-terminal half (aa 1-240) to many S-adenosyl-L-methionine-precorrin-2 methyl transferase (244 aa), and in C-terminal half (aa 240-508) to precorrin-3 methylase (254 aa). P9WGB3 No intermediary metabolism and respiration P66877 P9WGB3 GO:0030788,GO:0030789,GO:0009236 2.1.1.131,2.1.1.130 P9WGB3 NC_000962.3 Mycobrowser_v5 CDS 2333037 2333288 . - 0 Rv2076c Rv2076c Unknown Conserved hypothetical protein Rv2076c, (MTCY49.15c), len: 83 aa. Conserved hypothetical protein, similar to many. P9WLL3 No conserved hypotheticals P9WLL3 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2335355 2337325 . + 0 Rv2079 Rv2079 Unknown Conserved hypothetical protein Rv2079, (MTCY49.18), len: 656 aa. Conserved hypothetical protein; part of Mycobacterium tuberculosis protein family with Rv2542, Rv2077c, Rv2797c, Rv0963c, Rv1949c. Contains PS00120 Lipases, serine active site P9WLK7 No conserved hypotheticals Q10687 P9WLK7 P9WLK7 NC_000962.3 Mycobrowser_v5 CDS 2344411 2346180 . + 0 Rv2088 pknJ Involved in signal transduction (via phosphorylation). Can phosphorylate the peptide substrate myelin basic protein (MBP). [catalytic activity: ATP + a protein = ADP + a phosphoprotein]. Transmembrane serine/threonine-protein kinase J PknJ (protein kinase J) (STPK J) Rv2088, (MTCY49.28), len: 589 aa. PknJ, transmembrane serine/threonine-protein kinase (see citation below). Contains PS00108 Serine/Threonine protein kinases active-site signature. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases. Experimental studies show evidence of auto-phosphorylation. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Cofactor: requires divalent cations for activity. P9WI67 No regulatory proteins P65732 P9WI67 GO:0016021,GO:0005886,GO:0006468,GO:0004674,GO:0005524 2.7.11.1 P9WI67 NC_000962.3 Mycobrowser_v5 CDS 2349334 2352054 . - 0 Rv2092c helY DNA helicase activity. ATP-dependent DNA helicase HelY Rv2092c, (MTCY49.32c), len: 906 aa. HelY, ATP-dependent DNA helicase, similar to many; contains PS00017 ATP/GTP-binding site motif A, PS00402 Binding-protein-dependent transport systems inner membrane component signature. Belongs to the SKI2 subfamily of helicases. P9WMR1 No information pathways Q10701 P9WMR1 GO:0008026,GO:0003676,GO:0005524 3.6.4.- P9WMR1 NC_000962.3 Mycobrowser_v5 CDS 2358389 2360041 . + 0 Rv2100 Rv2100 Unknown Conserved hypothetical protein Rv2100, (MTCY49.40), len: 550 aa. Conserved hypothetical protein. Member of Mycobacterium tuberculosis REP13E12 repeat family with Rv1148c, Rv1945, Rv3467, Rv0094c, Rv1128c, Rv1587c, Rv1702c, Rv3466, Rv1588c. P9WLJ3 No insertion seqs and phages P64945 P9WLJ3 NC_000962.3 Mycobrowser_v5 CDS 2360240 2363281 . + 0 Rv2101 helZ Has helicase activity. Probable helicase HelZ Rv2101, (MTV020.01), len: 1013 aa. Probable helZ, helicase, similar to many. Nucleotide position 2361623 in the genome sequence has been corrected, A:C resulting in M462L. I6YCF3 No information pathways O53499 I6YCF3 GO:0003677,GO:0004386,GO:0005524 3.6.-.- I6YCF3 NC_000962.3 Mycobrowser_v5 CDS 2372630 2373823 . + 0 Rv2113 Rv2113 Unknown Probable integral membrane protein Rv2113, (MTCY261.09), len: 397 aa. Probable integral membrane protein. O33248 No cell wall and cell processes O33248 NC_000962.3 Mycobrowser_v5 CDS 2374461 2376290 . - 0 Rv2115c mpa Involved in proteasomal protein degradation Mycobacterial proteasome ATPase Mpa Rv2115c, (MTCY261.11c), len: 609 aa. Mpa, mycobacterial proteasome ATPase, similar to many. Contains PS00674 AAA-protein family signature and PS00017 ATP/GTP-binding site motif A (P-loop). Identified as a substrate for proteasomal degradation (See Pearce et al., 2006). Pup|Rv2111c and Mpa|Rv2115c interact (See Pearce et al., 2008). P9WQN5 No cell wall and cell processes 3FP9,3M91,3M9B,3M9D,3M9H P63345 P9WQN5 GO:0017111,GO:0009405,GO:0000502,GO:0005524 P9WQN5 NC_000962.3 Mycobrowser_v5 CDS 2382489 2386067 . - 0 Rv2124c metH Involved in biosynthesis of methionine (at the terminal step) [catalytic activity: 5-methyltetrahydrofolate + L-homocysteine = tetrahydrofolate + L-methionine]. 5-methyltetrahydrofolate--homocystein methyltransferase MetH (methionine synthase, vitamin-B12 dependent isozyme) (ms) Rv2124c, (MTCY261.20c), len: 1192 aa. MetH, methionine synthase, similar to many. Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Belongs to the vitamin-B12 dependent methionine synthase family. O33259 No intermediary metabolism and respiration O33259 O33259 GO:0008898,GO:0005622,GO:0009086,GO:0008705,GO:0042558,GO:0008270,GO:0031419 2.1.1.13 O33259 NC_000962.3 Mycobrowser_v5 CDS 2388616 2390085 . + 0 Rv2127 ansP1 Involved in L-asparagine transport. L-asparagine permease AnsP1 Rv2127, (MTCY261.26), len: 489 aa. AnsP1, L-asparagine permease, integral membrane protein similar to many. Contains PS00218 Amino acid permeases signature. Seems to belong to the APC family. P9WQM9 No cell wall and cell processes O33261 P9WQM9 GO:0006865,GO:0016021,GO:0005886,GO:0055085,GO:0015171 P9WQM9 NC_000962.3 Mycobrowser_v5 CDS 2390085 2390288 . + 0 Rv2128 Rv2128 Unknown Conserved transmembrane protein Rv2128, (MTCY26.27), len: 67 aa. Conserved transmembrane protein, similar to many. O33262 No cell wall and cell processes O33262 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2409697 2410641 . - 0 Rv2151c ftsQ This protein may be involved in septum formation. Possible cell division protein FtsQ Rv2151c, (MTCY270.17), len: 314 aa. Possible ftsQ, cell division protein, with some homology to FTSQ_STRGR|P45503 cell division protein ftsq homolog from Streptomyces griseus (208 aa), FASTA scores: opt: 204, E(): 4e-05; (30.6% identity in 193 aa overlap). P9WNA1 No cell wall and cell processes P64168 P9WNA1 GO:0007049,GO:0016021,GO:0005886,GO:0000917 P9WNA1 NC_000962.3 Mycobrowser_v5 CDS 2392517 2393320 . - 0 Rv2131c cysQ Can dephosphorylate a broad range of substrates. Likely involved in sulfur metabolism, controlling the pool of 3'-phosphoadenosine 5'-phosphate (pap) and 3'-phosphoadenoside 5'-phosphosulfate (PAPS) [catalytic activity: adenosine 3',5'-bisphosphate + H2O = adenosine 5'-phosphate + phosphate]. Has also been shown to have myo-inositol 1-phosphatase [catalytic activity: myo-inositol 1-phosphate + H(2)O = myo-inositol + phosphate] and fructose 1,6-bisphosphatase [catalytic activity: D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate] activities. Monophosphatase CysQ Rv2131c, (MTCY270.37), len: 267 aa. CysQ, monophosphatase, equivalent to CYSQ_MYCLE|P46726 cysQ protein homolog from Mycobacterium leprae (289 aa), FASTA scores: opt: 1374, E(): 0, (77.3% identity in 264 aa overlap). Contains inositol monophosphatase family signature 1 (PS00629), significance uncertain. Seems to belong to the inositol monophosphatase family. Cofactor: Mg2+. Inhibited by Li+; PAPase activity is inhibited by Na+ and K+, but IMPase activity is not (See Gu et al., 2006; Hatzios et al., 2008). P9WKJ1 No intermediary metabolism and respiration P65163 P9WKJ1 GO:0046872,GO:0004437 3.1.3.- P9WKJ1 NC_000962.3 Mycobrowser_v5 CDS 2396008 2396838 . - 0 Rv2136c Rv2136c Unknown Possible conserved transmembrane protein Rv2136c, (MTCY270.32), len: 276 aa. Possible conserved transmembrane protein, very similar to hypothetical Mycobacterium leprae protein Q49783. FASTA best: Q49783 B2126_C2_190 opt: 1023, E(): 0; (82.4% identity in 187 aa over lap) similar to BACA_ECOLI P31054 bacitracin resistance protein (273 aa) opt: 477, E(): 7e-26, (35.6% identity in 267 aa overlap) P9WFF9 No cell wall and cell processes O06239 P9WFF9 GO:0016311,GO:0016021,GO:0009252,GO:0005886,GO:0008360,GO:0046677,GO:0050380,GO:0007047 3.6.1.27 NC_000962.3 Mycobrowser_v5 CDS 2400376 2401722 . - 0 Rv2141c Rv2141c Unknown Conserved protein Rv2141c, (MTCY270.27), len: 448 aa. Conserved protein. Shows some similarity to conserved hypothetical proteins and to acetylornithine deacetylase and succinyl-diaminopimelate desuccinylase and contains ArgE/dapE/ACY1/CPG2/yscS family signature 1 (PS00758). FASTA best: CBPS_YEAST P27614 carboxypeptidases precursor (576 aa) opt: 234, E(): 4.3e-08; (24.3% identity in 412 aa overlap). Previously named dapE2. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). L7N684 No intermediary metabolism and respiration Q7D7G7 L7N684 GO:0005515,GO:0006508,GO:0008237 L7N684 NC_000962.3 Mycobrowser_v5 CDS 2417472 2419004 . - 0 Rv2157c murF Involved in cell wall formation; peptidoglycan biosynthesis. Probable UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanyl ligase MurF Rv2157c, (MTCY270.11), len: 510 aa. Probable murF, UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanyl ligase (UDP-murnac-pentapeptide synthetase) (see citation below), also related to other Mycobacterium tuberculosis mur gene products. FASTA best: MURF_ECOLI|P11880 (452 aa), opt: 515, E(): 2.6e-24, (31.9% identity in 511 aa overlap). P9WJL1 No cell wall and cell processes P0A5L4 P9WJL1 GO:0047480,GO:0008766,GO:0007049,GO:0051301,GO:0007047,GO:0005737,GO:0009252,GO:0008360,GO:0005524 6.3.2.10 P9WJL1 NC_000962.3 Mycobrowser_v5 CDS 2425048 2427087 . - 0 Rv2163c pbpB Involved in peptidoglycan biosynthesis Probable penicillin-binding membrane protein PbpB Rv2163c, (MTCY270.05), len: 679 aa. Probable pbpB, penicillin-binding membrane protein, similar to many bacterial PBP2 proteins e.g. P11882|PBP2_NEIME|PENA|NMA2072|NMB0413 penicillin-binding protein 2 (pbp-2) from Neisseria meningitidis (serogroups a and B) (581 aa), FASTA scores: opt: 665, E(): 1.6e-31, (33.2% identity in 591 aa overlap); etc. Also similar to Rv0016c and Rv2864c from Mycobacterium tuberculosis (2.8e-10). Contains PS00017 possible ATP/GTP-binding site motif A (P-loop) near C-terminus. FASTA best: PBP2_NEIME P11882 penicillin-binding protein 2 (pbp-2). (581 aa) opt: 665, E(): 1.6e-31; (33.2% identity in 591 aa overlap). FtsW|Rv2154c interacts with PbpB|Rv2163c and FtsZ|RvRv2150c (See Datta et al., 2006). Cleavage of PbpB|Rv2163c by Rv2869c under conditions of oxidative stress is prevented by Wag31|Rv2145c (See Mukherjee et al., 2009). L0T911 No cell wall and cell processes O06214 L0T911 GO:0009273,GO:0008658 L0T911 NC_000962.3 Mycobrowser_v5 CDS 2432235 2432855 . + 0 Rv2170 Rv2170 Acetylation, substrate unknown GCN5-related N-acetyltransferase Rv2170, (MTV021.03), len: 206 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain in C-terminal part. See Vetting et al. 2005. Equivalent to hypothetical protein ML0903 (210 aa) from Mycobacterium leprae. FASTA scores: ML0903 conserved hypothetical protein (210 aa) opt: 1045, E(): 9.1e-57; 77.143% identity in 210 aa overlap. >emb|CAA18679.1| (AL022602) >gi|13092973|emb|CAC31284.1| (AL583920). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53504 No intermediary metabolism and respiration O53504 O53504 GO:0008152,GO:0008080 O53504 NC_000962.3 Mycobrowser_v5 CDS 2626654 2626980 . - 0 Rv2348c Rv2348c Unknown Hypothetical protein Rv2348c, (MTCY98.17c), len: 108 aa. Hypothetical unknown protein. P95244 No unknown P95244 NC_000962.3 Mycobrowser_v5 CDS 2437941 2439140 . + 0 Rv2176 pknL Involved in signal transduction (via phosphorylation). May be involved in transcriptional mechanism. Probable transmembrane serine/threonine-protein kinase L PknL (protein kinase L) (STPK L) Rv2176, (MTV021.09), len: 399 aa. Probable pknL, transmembrane serine/threonine-protein kinase (see citation below), similar to many e.g. MLCB1770_9 (622 aa). Lacks C-terminal domain and ends with putative transmembrane segment. Contains PS00108 Serine/Threonine protein kinases active-site signature. FASTA scores: Z70722|MLC B1770_9 Mycobacterium leprae cosmid B1770 (622 aa) opt: 732, E(): 5.9e-23; 44.4% identity in 266 aa overlap. Also similar to several Mycobacterium tuberculosis STPK proteins e.g. Rv0014c|PKNB, Rv0015c|PKNA, Rv1743|PKNE, Rv1266c|PKNH etc. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases. P9WI63 No regulatory proteins O53510 P9WI63 GO:0016021,GO:0005886,GO:0006468,GO:0004674,GO:0005524 2.7.11.1 P9WI63 NC_000962.3 Mycobrowser_v5 CDS 2440332 2441720 . - 0 Rv2178c aroG Chorismate biosynthesis 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase AroG (DAHP synthetase, phenylalanine-repressible) Rv2178c, (MTV021.11c), len: 462 aa. aroG, 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase similar to many, especially those from plants. FASTA scores: Y15113|M C3DDAH7P_1Morinda citrifolia mRNA for 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (535 aa) opt: 1421, E(): 0; 48.3% identity in 443 aa overlap. O53512 No intermediary metabolism and respiration 2B7O,2W19,2W1A,3KGF,3NUD,3NUE,3NV8,3PFP O53512 O53512 GO:0009073,GO:0003849 2.5.1.54 O53512 NC_000962.3 Mycobrowser_v5 CDS 2444586 2445329 . - 0 Rv2182c Rv2182c Transfer of fatty acyl groups 1-acylglycerol-3-phosphate O-acyltransferase Rv2182c, (MTV021.15c), len: 247 aa. Probable 1-acylglycerol-3-phosphate O-acyltransferase, similar to many e.g. in Streptomyces. Contains PS00017 ATP/GTP-binding site motif A (P-loop). FASTA scores: pir||T35503 1-acylglycerol-3-phosphate O-acyltransferase homolog SC6E10.16c - Streptomyces coelicolor >gi|5689932|emb|CAB51970.1| (AL109661) hypothetical protein [Streptomyces coelicolor A3(2)] Length = 262, Expect = 6e-61 (54% identity in 215 aa overlap). O53516 No conserved hypotheticals O53516 O53516 GO:0008152,GO:0003841 2.3.1.51 O53516 NC_000962.3 Mycobrowser_v5 CDS 2461504 2462148 . - 0 Rv2197c Rv2197c Unknown Probable conserved transmembrane protein Rv2197c, (MTCY190.08c), len: 214 aa. Probable conserved transmembrane protein, equivalent to ML0878 conserved hypothetical protein (212 aa) of Mycobacterium leprae. FASTA scores: opt: 858; 62.559% identity in 211 aa overlap CAC31259.1|(AL583920). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WLI9 No cell wall and cell processes P9WLI9 GO:0005886,GO:0016021 P9WLI9 NC_000962.3 Mycobrowser_v5 CDS 3491808 3492122 . - 0 Rv3126c Rv3126c Unknown Hypothetical protein Rv3126c, (MTCY164.36c), unknown, len: 104 aa. Hypothetical unknown protein. Shortened version of MTCY164.36c, avoiding overlap. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WL09 No conserved hypotheticals P9WL09 EMB NC_000962.3 Mycobrowser_v5 CDS 2448160 2449962 . + 0 Rv2187 fadD15 Function unknown, but involved in lipid degradation. Long-chain-fatty-acid-CoA ligase FadD15 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv2187, (MTV021.20), len: 600 aa. fadD15, long-chain-fatty-acid-CoA ligase, similar to several e.g. P44446|LCFH_HAEIN putative long-chain-fatty-acid--CoA ligase from Haemophilus influenzae (607 aa), FASTA scores: (607 aa) opt: 992, E(): 0, (31.5% identity in 578 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family. O53521 No lipid metabolism O53521 O53521 GO:0008152,GO:0004467 6.2.1.3 O53521 NC_000962.3 Mycobrowser_v5 CDS 2453819 2455756 . + 0 Rv2191 Rv2191 Unknown Conserved hypothetical protein Rv2191, (MTCY190.02), len: 645 aa. Conserved hypothetical protein, similar to SW:DP3A_B ACSU P13267 DNA polymerase III, alpha chain (31.3% identity in 249 aa overlap) and SW:UVRC_ECOLI P07028 excinuclease ABC subunit C (25.7% identity in 230 aa overlap). Also similar to M. tuberculosis Rv3711c (dnaQ DNA polymerase III e chain) and Rv1420 (uvrC excinuclease ABC subunit C) P9WLJ1 No information pathways Q10384 P9WLJ1 GO:0006281,GO:0006260,GO:0003887,GO:0004527,GO:0005622,GO:0003677 P9WLJ1 NC_000962.3 Mycobrowser_v5 CDS 2459678 2461327 . + 0 Rv2196 qcrB Respiration Probable ubiquinol-cytochrome C reductase QcrB (cytochrome B subunit) Rv2196, (MTCY190.07), len: 549 aa. Probable qcrB, Ubiquinol-cytochrome C reductase cytochrome B subunit (cytB), integral membrane protein, low similarity in amino-terminal half to cytochrome b subunits, highly similar at C-terminus to SW:12KD_MYCLE P15878 12 KD protein PIR:S08427 (86.9% identity in 153 aa overlap). FASTA scores: sp|Q45658|QCRB_BACST menaquinol-cytochrome C reductase (224 aa) opt: 341, E(): 6.8e-15; 28.0% identity in 207 aa overlap P9WP37 No intermediary metabolism and respiration P9WP37 GO:0016021,GO:0046872,GO:0016491,GO:0005886,GO:0022904,GO:0006810,GO:0009055 NC_000962.3 Mycobrowser_v5 CDS 2687128 2687973 . + 0 Rv2393 che1 Possibly involved in inserting FE2+ into sirohydrochlorin to produce siroheme, required for SIRA (Rv2391) function Ferrochelatase Che1 Rv2393, (MTCY253.28c), len: 281 aa. Che1, ferrochelatase (See Pinto et al., 2007). Conserved protein, with some similarity to Q9L2E8|SC7A8.10c putative secreted protein from Streptomyces coelicolor (274 aa), FASTA scores: opt: 407, E(): 2.8e-18, (37% identity in 246 aa overlap); CAC38793|SCI39.05 Conserved hypothetical protein from Streptomyces coelicolor (305 aa), FASTA scores: opt: 394, E(): 2e-17, (35.0% identity in 251 aa overlap); AAK44492|MT0272 Chalcone/stilbene synthase family protein from Mycobacterium tuberculosis (247 aa), FASTA scores: opt: 350, E(): 9.2e-15, (34.0% identity in 235 aa overlap); P95216|Rv0259c|MTCY06A4.03c|Z86089 hypothetical protein from Mycobacterium tuberculosis (247 aa), FASTA scores: opt: 345, E(): 1.9e-14,(33.6% identity in 235 aa overlap). P71751 No intermediary metabolism and respiration P71751 P71751 GO:0016829,GO:0046872,GO:0009236 P71751 NC_000962.3 Mycobrowser_v5 CDS 2468231 2468923 . + 0 Rv2203 Rv2203 Unknown Possible conserved membrane protein Rv2203, (MTCY190.14), len: 230 aa. Possible conserved membrane protein; has single hydrophobic stretch from aa 75 to 97 and is equivalent to Mycobacterium leprae ML0872 putative membrane protein (171 aa). FASTA scores: opt: 821, E(): 3.4e-42; 72.353% identity in 170 aa overlap - CAC31253.1| (AL583920). 2468411. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WLI7 No cell wall and cell processes P9WLI7 GO:0016021 P9WLI7 NC_000962.3 Mycobrowser_v5 CDS 2477190 2478326 . + 0 Rv2212 Rv2212 Possibly involved in cAMP synthesis [catalytic activity: ATP = 3',5'-cyclic AMP + diphosphate] Adenylyl cyclase (ATP pyrophosphate-lyase) (adenylate cyclase) Rv2212, (MTCY190.23), len: 378 aa. Adenylyl cyclase (See Abdel Motaal et al., 2006). Some similarity to e.g. SW:CYAA_STRCO P40135 adenylate cyclase (29.2% identity in 291 aa overlap); ttg at 24614 in MTCY190 has a better rbs. Contains possible helix-turn-helix motif at aa 64- 85, (+2.72 SD). Also similar to Rv1264 and Rv1647 P9WMU7 No intermediary metabolism and respiration P64265 P9WMU7 GO:0009190,GO:0016849 P9WMU7 NC_000962.3 Mycobrowser_v5 CDS 2478338 2479885 . + 0 Rv2213 pepB Protein degradation Probable aminopeptidase PepB Rv2213, (MTCY190.24), len: 515 aa. Probable pepB, leucine aminopeptidase, similar to many e.g. SW:AMPA_ECOLI P11648 aminopeptidase a/I, (41.4% identity in 309 aa overlap). Equivalent to Z98741|MLCB22_6 Mycobacterium leprae cosmid B22; Am (524 aa), FASTA scores: opt: 2793, E(): 0; 83.1% identity in 522 aa overlap. Contains PS00631 Cytosol aminopeptidase signature, ntdaegrl. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P9WHT3 No intermediary metabolism and respiration Q10401 P9WHT3 GO:0005737,GO:0030145,GO:0008235,GO:0006508,GO:0004177 3.4.11.1 P9WHT3 NC_000962.3 Mycobrowser_v5 CDS 2481965 2483626 . + 0 Rv2215 dlaT Involved in tricarboxylic acid cycle; converts pyruvate to acetyl-CoA and CO2. Also involved in antioxidant defense; LPDC|Rv0462, DLAT|Rv2215, AHPD|Rv2429, and AHPC|Rv2428 constitute an NADH-dependent peroxidase and peroxynitrite reductase that provides protection against oxidative stress. DlaT, dihydrolipoamide acyltransferase, E2 component of pyruvate dehydrogenase Rv2215, (MTCY190.26), len: 553 aa. DlaT, dihydrolipoamide acyltransferase, E2 component of pyruvate dehydrogenase, proven biochemically (see Tian et al. 2005), similar to e.g. SW:O PD2_ACHLA P35489 dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex (35.3% identity in 552 aa overlap); contains PS00189 2-oxo acid dehydrogenases acyltransferase component lipoyl binding site. Rhodanine compounds inhibit DlaT|Rv2215 and can kill non-replicating mycobacteria in mouse bone marrow-derived macrophages (See Bryk et al., 2008). LpdC|Rv0462 co-immunoprecipitates with DlaT|Rv2215 (in lpdC|Rv0462 mutant) and with BkdC|Rv2495c (in dlaT|Rv2215 mutant) (See Venugopal et al., 2011). P9WIS7 No intermediary metabolism and respiration P65633 P9WIS7 GO:0004149,GO:0031405,GO:0005515,GO:0006099,GO:0016209 2.3.1.61 P9WIS7 NC_000962.3 Mycobrowser_v5 CDS 2487615 2489051 . + 0 Rv2220 glnA1 Involved in glutamine biosynthesis [catalytic activity: ATP + L-glutamate + NH(3) = ADP + glutamine + orthophosphate]. Glutamine synthetase GlnA1 (glutamine synthase) (GS-I) Rv2220, (MTCY190.31, MTCY427.01), len: 478 aa. glnA1, glutamine synthetase class I (see Tullius et al., 2001), similar to many e.g. GLNA_STRCO|P15106 from Streptomyces coelicolor, FASTA score: (71.4% identity in 475 aa overlap); etc. Also similar to three other potential glutamine synthetases in Mycobacterium tuberculosis: Rv2222c|glnA2, Rv2860c|glnA4, and Rv1878|glnA3. Contains PS00180 Glutamine synthetase signature 1, PS00181 Glutamine synthetase putative ATP-binding region signature, and PS00182 Glutamine synthetase class-I adenylation site. Belongs to the glutamine synthetase family. Note has shown to be essential for M. tuberculosis virulence. P9WN39 No intermediary metabolism and respiration 1HTO,1HTQ,2BVC,2WGS,2WHI P0A590 P9WN39 GO:0005737,GO:0004356,GO:0006542,GO:0009399,GO:0005524 6.3.1.2 P9WN39 NC_000962.3 Mycobrowser_v5 CDS 2489369 2492353 . - 0 Rv2221c glnE Regulatory protein involved in the regulation of glutamine synthetase activity. Adenylylation and deadenylylation of glutamine synthetase. Possibly regulates GLNB|Rv2919c [catalytic activity: ATP + [L-glutamate:ammonia ligase (ADP-forming)] = pyrophosphate + adenylyl-[L-glutamate:ammonia ligase (ADP-forming)]]. Glutamate-ammonia-ligase adenylyltransferase GlnE (glutamine-synthetase adenylyltransferase) Rv2221c, (MTCY190.32c, MTCY427.02c), len: 994 aa. glnE, glutamate-ammonia-ligase adenylyltransferase (see citations below), similar to others e.g. GLNE_ECOLI|P30870 glutamate-ammonia-ligase adenylyltransferase from Escherichia coli, FASTA score: (24.4% identity in 721 aa overlap); GLNE_HAEIN|P44419 Glutamate-ammonia-ligase adenylyltransferase from Haemophilus influenzae (981 aa), FASTA score: (28.1% identity in 199 aa overlap); etc. Note that initiation codon uncertain. P9WN27 No intermediary metabolism and respiration P69942 P9WN27 GO:0008882,GO:0005524 2.7.7.42 P9WN27 NC_000962.3 Mycobrowser_v5 CDS 2495461 2497023 . - 0 Rv2224c caeA Converts unknown esters to corresponding free acid and alcohol Probable carboxylesterase CaeA Rv2224c, (MTCY427.05c), len: 520 aa. Probable caeA, carboxylesterase; has signal sequence and lipoprotein motif at N-terminal end. Very similar to three proteases/peptidases from Streptomyces spp.: L42758, L42759, L27466. FASTA score: L4 2758|STMSLPD STMSLPD NID: g940302 - Streptomyces (539 aa) opt: 1032 E(): 0, (37.5% identity in 533 aa overlap). Similar to hypothetical protein SW:YZZE_ECOLI P34211 (27.7% identity in 412 aa overlap) and highly similar to Rv2224c and Rv2672 (49.3% identity in 507 aa overlap); contains PS00013, Prokaryotic membrane lipoprotein lipid attachment site, and PS00120 Lipases, serine active site. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). Predicted to be an outer membrane protein (See Song et al., 2008). P9WHR3 No cell wall and cell processes P65823 P9WHR3 P9WHR3 NC_000962.3 Mycobrowser_v5 CDS 2497742 2498587 . + 0 Rv2225 panB Involved in pantothenate biosynthesis. 3-methyl-2-oxobutanoate hydroxymethyltransferase PanB Rv2225, (MTCY427.06), len: 281 aa. panB, 3-methyl-2-oxobutanoate hydroxymethyltransferase, similar to PANB_ECOLI|P31057 3-methyl-2-oxobutanoate hydroxymethyltransferase from Escherichia coli (45.9% identity in 257 aa overlap). Identified as a substrate for proteasomal degradation (See Pearce et al., 2006). P9WIL7 No intermediary metabolism and respiration 1OY0 P0A5Q8 P9WIL7 GO:0005737,GO:0046872,GO:0015940,GO:0003864 2.1.2.11 P9WIL7 NC_000962.3 Mycobrowser_v5 CDS 2501644 2502738 . - 0 Rv2228c Rv2228c Unknown Multifunctional protein. Has RNASE H, alpha-ribazole phosphatase, and acid phosphatase activities. Rv2228c, (MTCY427.09c), len: 364 aa. Multifunctional protein with RNase H, alpha-ribazole phosphatase, and acid phosphatase activities. Some similarity to phosphoglycerate mutase and ribonuclease H. Similar to CAB88177.1|AL352972 putative bifunctional protein (ribonuclease H/phosphoglycerate mutase) from Streptomyces coelicolor A3(2) (497 aa); Smith-Waterman scores: 107 bits (424), Expect = 4e-41 Identities = 160/485 (32%). Also similar in C-terminal part to Rv2419c and Rv2135c. P9WLH5 No information pathways 3HST P64955 P9WLH5 GO:0004523,GO:0003676 P9WLH5 NC_000962.3 Mycobrowser_v5 CDS 2504605 2505699 . - 0 Rv2231c cobC Involved in cobalamin biosynthesis Possible aminotransferase CobC Rv2231c, (MTCY427.12c), len: 364 aa. Possible cobC, aminotransferase. Note that initiation codon uncertain. Similar to CobC aminotransferases e.g. sp|P21633|COBC_PSEDE COBC protein (333 aa) opt: 277, E(): 1.7e-11; 28.8% identity in 313 aa overlap and also to e.g. SW:HIS8_ECOLI P06986 histidinol-phosphate aminotransferase (27.0% identity in 289 aa overlap), contains PS00105 aminotransferases class-I pyridoxal-phosphate attachment site. Real Mycobacterium tuberculosis histidinol-phosphate aminotransferase, hisC, is Rv1600 (MTCY336.04c). P9WQ89 No intermediary metabolism and respiration P63500 P9WQ89 GO:0005737,GO:0030170,GO:0008483,GO:0009058 2.6.1.- P9WQ89 NC_000962.3 Mycobrowser_v5 CDS 2506278 2507153 . + 0 Rv2232 ptkA Involved in signal transduction (via phosphorylation). Can phosphorylate PTPA|Rv2234 and the peptide substrate myelin basic protein (MBP) [catalytic activity: ATP + a protein = ADP + a phosphoprotein] Protein tyrosine kinase transcriptional regulatory protein PtkA Rv2232, (MTCY427.13), len: 291 aa. PtkA, protein tyrosine kinase, similar to members of haloacid dehalogenase-like family from several bacteria and to putative phosphatases e.g. Q9I767 and AAK78398. Contains N-terminal extension. FASTA scores: Q9I767 hypothetical protein PA0065 (221 aa) opt: 439 E(): 3.2e-18; 38.679% identity (40.196% ungapped) in 212 aa overlap; >>tr|AAK78398 Predicted phosphatase, had family (216 aa) opt: 427, E(): 1.5e-17; 34.762% identity (35.437% ungapped) in 210 aa overlap. Replaces previous Rv2232 and Rv2233. Predicted to be an outer membrane protein (See Song et al., 2008). P9WPI9 No regulatory proteins P68911 P9WPI9 GO:0008967,GO:0008152 P9WPI9 NC_000962.3 Mycobrowser_v5 CDS 2512539 2515244 . + 0 Rv2241 aceE Involved in energy metabolism; contributes to acetyl-CoA production as part of pyruvate dehydrogenase complex [catalytic activity: pyruvate + lipoamide = S-acetyl-dihydro-lipoamide + CO(2)]. Pyruvate dehydrogenase E1 component AceE (pyruvate decarboxylase) (pyruvate dehydrogenase) (pyruvic dehydrogenase) Rv2241, (MTCY427.22), len: 901 aa. AceE, pyruvate dehydrogenase E1 component, similar to others e.g. ODP1_ECOLI|P06958 pyruvate dehydrogenase E1 component from Escherichia coli, FASTA score: (51.2% identity in 891 aa overlap); etc. P9WIS9 No intermediary metabolism and respiration Q10504 P9WIS9 GO:0055114,GO:0004739,GO:0006096 1.2.4.1 P9WIS9 NC_000962.3 Mycobrowser_v5 CDS 2516787 2517695 . + 0 Rv2243 fabD Catalyzes malonyl-CoA-ACP transacylase (MCAT) activity using holo-ACPM as substrate for transacylation [catalytic activity: malonyl-CoA + [acyl-carrier protein] = CoA + malonyl-[acyl-carrier protein]]. Malonyl CoA-acyl carrier protein transacylase FabD (malonyl CoA:ACPM acyltransferase) (MCT) Rv2243, (MTCY427.24), len: 302 aa. FabD (alternate gene name: mtFabD), malonyl CoA-acyl carrier protein transacylase (see citations below), highly similar to e.g. A57356 acyl-CoA carrier protein malonyltransferase from Streptomyces coelicolor (316 aa), FASTA score: opt: 955, E(): 0, (52.6% identity in 304 aa overlap); FABD_HAEIN|P43712 malonyl CoA-acyl carrier protein transacylase from Haemophilus influenzae, FASTA score: (30.5% identity in 308 aa overlap); and FABD_ECOLI|P25715 from Escherichia coli, FASTA score: (31.4% identity in 309 aa overlap). Identified as a substrate for proteasomal degradation (See Pearce et al., 2006). P9WNG5 No lipid metabolism 2QC3,2QJ3 P63458 P9WNG5 GO:0006633,GO:0004314 2.3.1.39 INH P9WNG5 NC_000962.3 Mycobrowser_v5 CDS 2572076 2573008 . - 0 Rv2300c Rv2300c Function unknown Conserved protein Rv2300c, (MTCY339.09), len: 310 aa (start uncertain). Conserved protein, similar to others e.g. Q9RXY2|DR0172 conserved hypothetical protein from Deinococcus radiodurans (271 aa), FASTA scores: opt: 306, E(): 1.3e-12, (34.6% identity in 229 aa overlap); Q9HZH1|PA3037 hypothetical protein from Pseudomonas aeruginosa (288 aa), FASTA scores: opt: 248, E(): 7.9e-09, (31.5% identity in 238 aa overlap); Q9PDL8|XF1361 hypothetical protein from Xylella fastidiosa (279 aa), FASTA scores: opt: 236, E(): 4.6e-08, (29.7% identity in 249 aa overlap); U70053|XCU70053_3 GumP protein from Xanthomonas campestris (282 aa), FASTA scores: opt: 222, E(): 3.7e-07, (30.1% identity in 248 aa overlap); etc. P9WLD7 No conserved hypotheticals P9WLD7 GO:0016787 P9WLD7 NC_000962.3 Mycobrowser_v5 CDS 2519396 2520712 . + 0 Rv2246 kasB Involved in fatty acid biosynthesis (mycolic acids synthesis); involved in meromycolate extension. Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP [catalytic activity: acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = 3-oxoacyl-[acyl-carrier protein] + [acyl-carrier protein] + CO(2)]. 3-oxoacyl-[acyl-carrier protein] synthase 2 KasB (beta-ketoacyl-ACP synthase) (KAS I) Rv2246, (MTCY427.27), len: 438 aa. KasB, beta-ketoacyl-ACP synthase, involved in meromycolate extension (see citations below). Highly similar or similar to others e.g. L43074|STMFABD3|g870805 beta-ketoacyl-ACP synthase from Streptomyces glaucescens (423 aa), FASTA scores: opt: 1091, E(): 0, (44.7% identity in 416 aa overlap); FABF_ECOLI|P39435 3-oxoacyl-[acyl-carrier-protein] synthase II from Escherichia coli, FASTA score: (37.0% identity in 411 aa overlap); FABB_HORVU|P23902 3-oxoacyl-[acyl-carrier-protein] synthase I, FASTA score: (32.5% identity in 415 aa overlap); etc. Strongest similarity to upstream ORF Rv2245|kasA|MTCY427.26 3-oxoacyl-[acyl-carrier-protein] synthase 1 from Mycobacterium tuberculosis (416 aa), FASTA score: (66.3% identity in 409 aa overlap). Belongs to the beta-ketoacyl-ACP synthases family. P9WQD7 No lipid metabolism 2GP6 P63456 P9WQD7 GO:0005737,GO:0006633,GO:0004315 2.3.1.41 P9WQD7 NC_000962.3 Mycobrowser_v5 CDS 2525565 2526992 . + 0 Rv2251 Rv2251 Electron acceptor Possible flavoprotein Rv2251, (MTV022.01), len: 475 aa. Possible flavoprotein, probably continuation of Rv2250A, similar to MTCY164.18 from Mycobacterium tuberculosis and to several alkyldihydroxyacetonephosphate synthases (e.g. O00116). Also some similarity to D-lactate dehydrogenases. FASTA scores: sptr|O05784|O05784 hypothetical 56.5 kDa protein. (527 aa) opt: 1019 E(): 0; (38.6% identity in 487 aa overlap) and sp|O00116|ADAS_HUMAN alkyldihydroxyaceton ephosphate synthase precursor (658 aa) opt: 558 E(): 6.2e-27; (31.3% identity in 447 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). L0TBR2 No intermediary metabolism and respiration O53525 L0TBR2 GO:0016491,GO:0050660 L0TBR2 NC_000962.3 Mycobrowser_v5 CDS 2532245 2533330 . + 0 Rv2259 mscR Involved in the reduction of S-nitroglutathione S-nitrosomycothiol reductase MscR Rv2259, (MTV022.09), len: 361 aa. MscR, S-nitrosomycothiol reductase (see Vogt et al., 2003), similar to several zinc-containing alcohol dehydrogenases especially from Amycolatopsis methanolica P80094 (360 aa), FASTA scores: sp|P80094|FADH_AMYME NAD/mycothiol-dependent formaldehyde dehydrogenase (MD-FALDH) Length = 360, Expect = e-156, Identities = 268/358 (74%). Also similar to Rv0162c, (MTCI28.02c, 35.0% identity in 371 aa overlap). Contains PS00059 Zinc-containing alcohol dehydrogenases signature. Note previously known as adhE2 O53533 No intermediary metabolism and respiration O53533 O53533 GO:0016491,GO:0008270,GO:0055114 1.2.1.- O53533 NC_000962.3 Mycobrowser_v5 CDS 2534470 2535552 . - 0 Rv2262c Rv2262c Function unknown; thought to be involved in lipid metabolism. Conserved hypothetical protein Rv2262c, (MTV022.12c), len: 360 aa. Conserved hypothetical protein, with function unknown but some similarity to N-terminal 70% of P23930|P77703|LNT_ECOLI|cute|B0657 apolipoprotein N-acyltransferase from Escherichia coli strain K12 (512 aa), FASTA scores: opt: 239, E(): 1.6e-07, (30.4% identity in 359 aa overlap). Note that neighboring ORF shows similarity to N -terminal part of PCC6803 apolipoprotein N-acyltransferase from Synechocystis sp., suggesting possibility of frameshift. Sequence of clones from two sources has been checked but no error found. Appear to be two extra bases at position 1876970 compared to CDC1551 strain. O53536 No lipid metabolism O53536 O53536 GO:0016810,GO:0016021,GO:0042158,GO:0006807,GO:0016410 O53536 NC_000962.3 Mycobrowser_v5 CDS 2540104 2541390 . + 0 Rv2266 cyp124 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Probable cytochrome P450 124 Cyp124 Rv2266, (MT2328, MTCY339.44c), len: 428 aa. Probable cyp124, cytochrome P450, similar to e.g. G405543 cytochrome P450 (406 aa), FASTA scores, opt: 763,E(): 0, (35.4% identity in 393 aa overlap), similar to e.g. MTCY50.26, 33.8% identity in 370 aa overlap P9WPP3 No intermediary metabolism and respiration 2WM4,2WM5 P0A516 P9WPP3 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPP3 NC_000962.3 Mycobrowser_v5 CDS 2553173 2554831 . + 0 Rv2281 pitB Involved in phosphate transport. Putative phosphate-transport permease PitB Rv2281, (MTCY339.29c), len: 552 aa. Putative pitB, phosphate-transport permease, integral membrane protein, similar to YG04_HAEIN P45268 putative phosphate permease hi1604 (420 aa). FASTA scores, opt: 484, E(): 5e-23, (33.5% identity in 498 aa overlap) also to G399598 amphotropic murine retrovirus receptor (656 aa) FASTA scores, opt: 453, E(): 5.8e-21, (26.8% identity in 645 aa overlap). Also similar to Rv0545c|pitA from M. tuberculosis. Belongs to the pit subfamily. P9WIA5 No cell wall and cell processes P65712 P9WIA5 GO:0016021,GO:0006817,GO:0005886,GO:0005315 NC_000962.3 Mycobrowser_v5 CDS 2561675 2562457 . + 0 Rv2289 cdh Involved in phospholipid biosynthesis [catalytic activity: CDP-diacylglycerol + H(2)O = CMP + phosphatidate]. Probable CDP-diacylglycerol pyrophosphatase Cdh (CDP-diacylglycerol diphosphatase) (CDP-diacylglycerol phosphatidylhydrolase) Rv2289, (MTCY339.21c), len: 260 aa. Probable cdh, CDP-diacylglycerol pyrophosphatase, similar to CDH_SALTY|P26219 cdp-diacylglycerol pyrophosphatase (251 aa), FASTA scores: opt: 395, E(): 5.9e-20, (33.5% identity in 221 aa overlap). P9WPG9 No lipid metabolism P63751 P9WPG9 GO:0016021,GO:0008654,GO:0005886,GO:0008715 3.6.1.26 P9WPG9 NC_000962.3 Mycobrowser_v5 CDS 2565327 2566550 . + 0 Rv2294 Rv2294 Unknown Probable aminotransferase Rv2294, (MTCY339.16c), len: 407 aa. Probable aminotransferase, similar to others in M. tuberculosis e.g. MTV030_19, also similar to PATB_BACSU|Q08432 putative aminotransferase b from Bacillus subtilis (387 aa), FASTA scores: opt: 563, E(): 2.8e-29, (31.4% identity in 408 aa overlap); and to MALY_ECOLI|P23256 maly protein from Escherichia coli (390 aa), FASTA scores: opt: 530, E(): 3.6e-27, (31.3% identity in 384 aa overlap). Belongs to class-II of pyridoxal-phosphate-dependent aminotransferases. P9WQ83 No intermediary metabolism and respiration P63502 P9WQ83 GO:0009086,GO:0030170,GO:0016769,GO:0004121 4.4.1.8 P9WQ83 NC_000962.3 Mycobrowser_v5 CDS 2570059 2572002 . - 0 Rv2299c htpG Molecular chaperone involved in protein folding. Has ATPase activity. Probable chaperone protein HtpG (heat shock protein) (HSP90 family protein) (high temperature protein G) Rv2299c, (MTCY339.11), len: 647 aa. HtpG, probable chaperone, heat shock protein 90 family. Similar to HTPG_BACSU|P46208 heat shock protein htpG homologue from Bacillus subtilis (626 aa), FASTA scores: opt: 1551, E(): 0, (39.6% identity in 631 aa overlap). Contains possible helix-turn-helix motif at aa 519-540 (+3.77 SD). P9WMJ7 No virulence, detoxification, adaptation P64411 P9WMJ7 GO:0005737,GO:0006457,GO:0006950,GO:0051082,GO:0005524 P9WMJ7 NC_000962.3 Mycobrowser_v5 CDS 2574096 2575019 . - 0 Rv2303c Rv2303c Could be involved in antibiotic-resistance. Probable antibiotic-resistance protein Rv2303c, (MTCY339.06, MT2360), len: 307 aa. Probable antibiotic-resistance protein, with some similarity to Q54229|G153373 macrotetrolide antibiotic-resistance protein (NONR) from Streptomyces griseus (347 aa) (see Plater and Robinson, 1992), FASTA scores: opt: 438, E(): 3.1e-21, (33.2% identity in 226 aa overlap); and other hypothetical proteins e.g. P95886 ORF C02006 from Sulfolobus solfataricus (269 aa), FASTA scores: opt: 252, E(): 3.5e-09, (25.5% identity in 286 aa overlap); etc. Also similar to Mycobacterium tuberculosis Rv3510c|O53555|MTV023.17. Note that the protein Q9XDF3|NONC from Streptomyces griseus subsp. griseus (317 aa) is equivalent to Q54229|G153373|NONR however the N-terminal end is shorter (30 aa) owing to a changed start codon (see Walczak et al., 2000). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q50662 No virulence, detoxification, adaptation Q50662 Q50662 GO:0008152,GO:0003824 Q50662 NC_000962.3 Mycobrowser_v5 CDS 2577108 2577701 . + 0 Rv2306A Rv2306A Unknown Possible conserved membrane protein Rv2306A, len: 197 aa. Possible conserved membrane protein, similar to several hypothetical membrane proteins from Mycobacterium tuberculosis and Streptomyces coelicolor, e.g. Rv0625c|P96915|Y625_MYCTU hypothetical 25.2 KDA protein from Mycobacterium tuberculosis (246 aa), FASTA scores: opt: 410, E(): 2.7e-17, (53.25% identity in 139 aa overlap). First 140 aa show high similarity, this then decreases but continues in next ORF Rv2306B, suggesting a frameshift near nt 2577473. However the sequence has been checked and no error found. The sequence is identical in CDC1551 and Mycobacterium bovis. Replaces original Rv2306c on other strand. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q79FG5 No cell wall and cell processes Q79FG5 NC_000962.3 Mycobrowser_v5 CDS 2583435 2583779 . + 0 Rv2310 Rv2310 Use for sequence excision. Possible excisionase Rv2310, (MT2372, MTCY3G12.24c), len: 114 aa. Possible excisionase, showing some similarity to others e.g. Q9LCU5 putative excisionase from Arthrobacter sp. TM1 (174 aa) FASTA scores: opt: 341, E(): 6.6e-15, (48.2% identity in 110 aa overlap); O85865 putative excisionase from Sphingomonas aromaticivorans (152 aa), FASTA scores: opt: 205, E(): 2.2e-06, (41.25% identity in 80 aa overlap); etc. Also similar to Rv3750c|O69717 hypothetical protein from Mycobacterium tuberculosis (130 aa), FASTA scores: opt: 228, E(): 6.9e-08, (43.9% identity in 82 aa overlap). Contains possible helix-turn-helix motif at aa 20-41 (Score 2181, +6.62 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLC3 No insertion seqs and phages P9WLC3 GO:0000166,GO:0003677 NC_000962.3 Mycobrowser_v5 CDS 2587287 2588804 . - 0 Rv2315c Rv2315c Function unknown Conserved protein Rv2315c, (MTCY3G12.19), len: 505 aa. Conserved protein, highly similar to Q9S273|SCI28.10 hypothetical 47.1 KDA protein from Streptomyces coelicolor (435 aa), FASTA scores: opt: 1768, E():5.6e-101, (63.2% identity in 432 overlap); and similar to others e.g. AAK24787|CC2823 hypothetical protein (TldD/PmbA family) from Caulobacter crescentus (543 aa), FASTA scores: opt: 876, E():3.1e-46, (42.8% identity in 505 overlap); O58578|PH0848 hypothetical 54.4 KDA protein from Pyrococcus horikoshii (481 aa), FASTA scores: opt: 661, E(): 4.3e-33, (29.95% identity in 484 aa overlap); Q9UZ95|PAB1547 hypothetical 53.6 KDA protein from Pyrococcus abyssi (473 aa), FASTA scores: opt: 656, E(): 8.6e-33, (29.1% identity in 481 aa overlap); etc. P71897 No conserved hypotheticals P71897 P71897 NC_000962.3 Mycobrowser_v5 CDS 2588838 2589710 . + 0 Rv2316 uspA Thought to be involved in active transport of sugar across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable sugar-transport integral membrane protein ABC transporter UspA Rv2316, (MTCY3G12.18c), len: 290 aa. Probable uspA, sugar-transport integral membrane protein ABC transporter (see citation below), most similar to Q9CBN8|USPA|ML1768 sugar transport integral membrane protein from Mycobacterium leprae (328 aa), FASTA scores: opt: 1593, E(): 1.9e-93, (82.35% identity in 289 aa overlap); and similar to O32940|ML1426|MLCB2052.28 possible sugar transport protein (probable ABC-transport protein, inner membrane component) from Mycobacterium leprae (319 aa), FASTA scores: opt: 600, E(): 9.2e-31, (34.25% identity in 295 aa overlap). Also similar to other proteins involved in transport e.g. Q9X860|SCE134.05c putative binding protein dependent transport protein from Streptomyces coelicolor (327 aa), FASTA scores: opt: 639, E(): 3.2e-33, (40.45% identity in 272 aa overlap); Q9K6N9|BH3689 sugar transport system (permease) from Bacillus halodurans (300 aa), FASTA scores: opt: 590, E(): 3.7e-30, (35.65% identity in 289 aa overlap); etc. P71896 No cell wall and cell processes P71896 P71896 GO:0005886,GO:0006810,GO:0005215,GO:0016021 P71896 NC_000962.3 Mycobrowser_v5 CDS 2594154 2594699 . - 0 Rv2321c rocD2 Involved in arginine metabolism [catalytic activity: L-ornithine + a 2-oxo acid = L-glutamate 5-semialdehyde + an L-amino acid]. Probable ornithine aminotransferase (C-terminus part) RocD2 (ornithine--oxo-acid aminotransferase) Rv2321c, (MTCY3G12.13), len: 181 aa. Probable rocD2, ornithine aminotransferase, highly similar to C-terminal region of other ornithine aminotransferases, e.g. Q9FC90|ROCD from Streptomyces coelicolor (407 aa), FASTA scores: opt: 628, E(): 1.2e-32, (55.35% identity in 168 aa overlap); P3802|OAT_BACSU|ROCD from Bacillus subtilis (401 aa), FASTA scores: opt: 477, E(): 4.3e-23, (42.1% identity in 178 aa overlap); BAB42057|ROCD|SA0818 from Staphylococcus aureus subsp. aureus N315 (396 aa), FASTA scores: opt: 437, E(): 1.5e-20, (41.3% identity in 170 aa overlap); etc. Contains PS00600 Aminotransferases class-III pyridoxal-phosphate attachment site. Belongs to class-III of pyridoxal-phosphate-dependent aminotransferases. Rv2322c|MTCY3G12.12 (upstream ORF) and Rv2321c|MTCY3G12.13 appear to be an ornithine aminotransferase homologue but are frameshifted - we can find no sequence error in the cosmid to account for this. P71891 No intermediary metabolism and respiration P71891 P71891 GO:0030170,GO:0004587 2.6.1.13 P71891 NC_000962.3 Mycobrowser_v5 CDS 2597009 2597857 . - 0 Rv2325c Rv2325c Function unknown Conserved hypothetical protein Rv2325c, (MTCY3G12.09), len: 282 aa. Conserved hypothetical protein, equivalent to O32970|MLCB22.37c|ML0849 hypothetical protein from Mycobacterium leprae (283 aa), FASTA scores: opt: 1405, E(): 1.8e-78, (77.7% identity in 282 aa overlap). Also some similarity to other proteins e.g. Q9Z9J1|YBAF|BH0166 YBAF protein (BH0166 protein) (hypothetical protein) from Bacillus halodurans (265 aa), FASTA scores: opt: 288, E(): 2.8e-10, (25.8% identity in 264 aa overlap); P70972|YBAF YBAF protein (hypothetical protein) from Bacillus subtilis (265 aa), FASTA scores: opt: 259, E(): 1.5e-08, (25.45% identity in 224 aa overlap); AAK34821|SPY2193|Q99X13 Conserved hypothetical protein from Streptococcus pyogenes (266 aa), FASTA scores: opt: 232, E(): 6.5e-07, (25.1% identity in 267 aa overlap); etc. P9WPI7 No conserved hypotheticals P64997 P9WPI7 GO:0015087,GO:0006824,GO:0016021,GO:0005886,GO:0009236 NC_000962.3 Mycobrowser_v5 CDS 2644320 2645774 . + 0 Rv2363 amiA2 Generates monocarboxylate from monocarboxylic acid amide [catalytic activity: a monocarboxylic acid amide + H(2)O = a monocarboxylate + NH(3)]. Probable amidase AmiA2 (aminohydrolase) Rv2363, (MTCY27.17c), len: 484 aa. Probable amiA2, amidase, highly similar or similar to others e.g. O28325|YJ54_ARCFU|AF1954 putative amidase from Archaeoglobus fulgidus (453 aa), FASTA scores: opt: 777, E(): 1.1e-38, (35.0% identity in 474 aa overlap); Q55424|AMID_SYNY3|SLL0828 putative amidase from Synechocystis sp. strain PCC 6803 (506 aa), FASTA scores: opt: 770, E(): 3e-38, (36.4% identity in 456 aa overlap); Q53116|AMDA enantiomerase-selective amidase from Rhodococcus sp. (462 aa), FASTA scores: opt: 701, E(): 3.5e-34, (32.7% identity in 468 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. AMI2_MYCTU|AMIB2|Q11056|Rv1263|MT1301|MTCY50.19c|cy50.19c amidase (462 aa), FASTA scores: opt: 1141, E(): 2.9e-60, (45.4% identity in 454 aa overlap); etc. Contains PS00571 Amidases signature, and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the amidase family. P9WQ99 No intermediary metabolism and respiration P63490 P9WQ99 GO:0016884,GO:0004040 3.5.1.4 P9WQ99 NC_000962.3 Mycobrowser_v5 CDS 2597854 2599947 . - 0 Rv2326c Rv2326c Probably involved in active transport accross the membrane. Thought to be responsible for energy coupling to the transport system and the translocation of the substrate across the membrane. Possible transmembrane ATP-binding protein ABC transporter Rv2326c, (MTC3G12.08), len: 697 aa. Possible transmembrane ATP-binding protein ABC transporter (see citation below). Equivalent to Q9CCF9|ML0848 ABC transporter from Mycobacterium leprae (724 aa), FASTA scores: opt: 3482, E(): 2.8e-182, (76.9% identity in 697 aa overlap) and also to O32971|MLCB22.38c ABC-type transporter from Mycobacterium leprae (726 aa), FASTA scores: opt: 3482, E(): 2.8e-182, (76.9% identity in 697 aa overlap). Similar in part to other ABC transporters e.g. Q9WY65|TM0222 from Thermotoga maritima (266 aa), FASTA scores: opt: 407, E(): 4.2e-15, (38.0% identity in 213 aa overlap); etc. Contains 2 X PS00017 ATP/GTP-binding site motif A (P-loop); and 2 x PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). P9WQI7 No cell wall and cell processes P63399 P9WQI7 GO:0016887,GO:0016021,GO:0005886,GO:0006810,GO:0005524 P9WQI7 NC_000962.3 Mycobrowser_v5 CDS 2605108 2606754 . + 0 Rv2332 mez Catalyzes the oxidative decarboxylation of malate into pyruvate, important for a wide range of metabolic pathways [catalytic activity: (S)-malate + NAD(+) = pyruvate + CO(2) + NADH]. Probable [NAD] dependent malate oxidoreductase Mez (malic enzyme) (NAD-malic enzyme) (malate dehydrogenase (oxaloacetate decarboxylating)) (pyruvic-malic carboxylase) (NAD-me) Rv2332, (MTCY3G12.02c, MTCY98.01, MT2394), len: 548 aa. Probable mez, malate oxidoreductase [NAD] dependent (malic enzyme), highly similar to others e.g. O34389|MALS putative malolactic enzyme [includes: malic enzyme ; L-lactate dehydrogenase] from Bacillus subtilis (566 aa), FASTA scores: opt: 1927, E(): 5.5e-111, (52.9% identity in 539 aa overlap); P45868|MAO2_BACSU|YWKA probable NAD-dependent malic enzyme from Bacillus subtilis (582 aa), FASTA scores: opt: 1849, E(): 3.6e-106, (50.45% identity in 543 aa overlap); Q48796|MLES_OENOE malolactic enzyme from Oenococcus oeni (541 aa), FASTA scores: opt: 1540, E(): 3.6e-87, (44.2% identity in 536 aa overlap); etc. Belongs to the malic enzymes family. N-terminus shortened since first submission (previously 652 aa). P9WK25 No intermediary metabolism and respiration P71880 P9WK25 GO:0016619,GO:0006108,GO:0046872,GO:0055114,GO:0051287 1.1.1.38 P9WK25 NC_000962.3 Mycobrowser_v5 CDS 2608796 2609728 . + 0 Rv2334 cysK1 Involved in cysteine biosynthesis [catalytic activity: O3-acetyl-L-serine + H(2)S = L-cysteine + acetate]. Cysteine synthase a CysK1 (O-acetylserine sulfhydrylase A) (O-acetylserine (thiol)-lyase A) (CSASE A) Rv2334, (MT2397, MTCY98.03), len: 310 aa. cysK1, cysteine synthase A, equivalent to O32978|CYSK_MYCLE|ML0839|MLCB22.47 cysteine synthase a from Mycobacterium leprae (310 aa), FASTA scores: opt: 1756, E(): 8.6e-96, (85.8% identity in 310 aa overlap). Also highly similar to other cysteine synthases e.g. Q9JQL6|CYSK|NMA0974|NMB0763 putative cysteine synthase from Neisseria meningitidis (serogroup a and B) (310 aa), FASTA scores: opt: 1368, E(): 4.6e-73, (66.45% identity in 310 aa overlap); P73410|CYSK_SYNY3|SLR1842 from Synechocystis sp (312 aa), FASTA scores: opt: 1310, E(): 1.2e-69, (64.65% identity in 311 aa overlap); Q43725|CYSM_ARATH|OASC|ACS1|AT3G59760|F24G16.30 cysteine synthase (mitochondrial precursor) from Arabidopsis thaliana (Mouse-ear cress) (424 aa), FASTA scores: opt: 1253, E(): 3.2e-66, (59.2% identity in 309 aa overlap) (has its N-terminus longer 104 aa); etc. Contains PS00901 Cysteine synthase/cystathionine beta-synthase P-phosphate attachment site. Belongs to the cysteine synthase/cystathionine beta-synthase family. Note that previously known as cysK. P9WP55 No intermediary metabolism and respiration 2Q3B,2Q3C,2Q3D P0A534 P9WP55 GO:0004124,GO:0030170,GO:0016740,GO:0006535 2.5.1.47 P9WP55 NC_000962.3 Mycobrowser_v5 CDS 2613107 2614063 . - 0 Rv2338c moeW Involved in molybdoptenum cofactor biosynthesis; thought to be involved in the biosynthesis of a demolybdo-cofactor (molybdopterin), necessary for molybdo-enzymes. Possible molybdopterin biosynthesis protein MoeW Rv2338c, (MTCY98.07c), len: 318 aa. Possible moeW, molybdoptenum biosynthesis protein, showing some similarity to several molybdopterin biosynthesis proteins e.g. O27613|MTH1571 molybdopterin biosynthesis protein MOEB homolog from Methanobacterium thermoautotrophicum (251 aa), FASTA scores: opt: 309, E(): 4.7e-14; (30.7% identity in 254 aa overlap); Q9KPQ5|VC2311 HESA/MOEB/THIF family protein from Vibrio cholerae (273 aa), FASTA scores: opt: 255, E(): 4e-09, (36.25% identity in 149 aa overlap); Q9PD34|XF1545 molybdopterin biosynthesis protein from Xylella fastidiosa (276 aa), FASTA scores: opt: 233,E(): 1e-07, (33.6% identity in 128 aa overlap); etc. Seems to belong to the HESA/MOEB/THIF family. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P95234 No intermediary metabolism and respiration P95234 P95234 GO:0003824,GO:0008152 P95234 NC_000962.3 Mycobrowser_v5 CDS 2620533 2622452 . - 0 Rv2343c dnaG DNA primase is the polymerase that synthesizes small RNA primers for the okazaki fragments on both template strands replication FORKS during chromosomal DNA synthesis. Probable DNA primase DnaG Rv2343c, (MTCY98.12c), len: 639 aa. Probable dnaG, DNA primase, equivalent to O52200|PRIM_MYCSM|DNAG DNA primase from Mycobacterium smegmatis (636 aa), FASTA scores: opt: 3504, E(): 5.5e-202, (81.55% identity in 639 aa overlap); and Q9CCG2|DNAG|ML0833 DNA primase from Mycobacterium leprae (642 aa), FASTA scores: opt: 3443, E(): 2.5e-198, (80.4% identity in 642 aa overlap). Also highly similar to many DNA primases e.g. Q9S1N4|PRIM_STRCO|DNAG|SC7A8.07c from Streptomyces coelicolor (641 aa), FASTA scores: opt: 1899, E(): 5.1e-106, (47.9% identity in 643 aa overlap); P74893|PRIM_SYNP7|DNAG from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (616 aa), FASTA scores: opt: 860, E(): 6.6e-44, (35.3% identity in 513 aa overlap); P05096|PRIM_BACSU from Bacillus subtilis (603 aa) FASTA scores: opt: 800, E(): 2.5e-40, (33.7% identity in 430 aa overlap); etc. P9WNW1 No information pathways P63962 P9WNW1 GO:0003896,GO:0006269,GO:0005658,GO:0006350,GO:0008270,GO:0003677 2.7.7.- P9WNW1 NC_000962.3 Mycobrowser_v5 CDS 2627172 2628698 . - 0 Rv2349c plcC Hydrolyzes sphingomyelin in addition to phosphatidylcholine. Probable virulence factor implicated in the pathogenesis of Mycobacterium tuberculosis at the level of intracellular survival, by the alteration of cell signaling events or by direct cytotoxicity [catalytic activity: a phosphatidylcholine + H(2)O = 1,2- diacylglycerol + choline phosphate]. Probable phospholipase C 3 PlcC Rv2349c, (MT2414, MTCY98.18c), len: 508 aa. Probable plcC, phospolipase C 3 (see citations below), similar to other precursors of several phospolipases C e.g. P15713|PHLN_PSEAE|PA3319 non-hemolytic phospholipase C precursor from Pseudomonas aeruginosa (692 aa), FASTA scores: opt: 1013, E(): 9.3e-54, (38.85% identity in 525 aa overlap); P06200|PHLC_PSEAE hemolytic phospholipase C precursor from Pseudomonas aeruginosa (730 aa), FASTA scores: opt: 630, E(): 1.5e-30, (35.15% identity in 535 aa overlap); Q9S816|T12J13.18|T21P5.4 putative phospholipase from Arabidopsis thaliana (Mouse-ear cress) (521 aa), FASTA scores: opt: 218, E(): 1e-05, (27.05% identity in 451 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. Q9XB13|PLCD|Rv1755c|MT1799|MTCY28.21C phospholipase C 4 (514 aa), FASTA scores: opt: 2497, E(): 9e-144, (68.35% identity in 509 aa overlap); Q50560|Rv2351c|PLCA|MTP40|MT2416|MTCY98.20c phospholipase C 1 (520 aa), FASTA scores: opt: 2494, E(): 1.4e-143, (68.1% identity in 514 aa overlap); P95246|PLCB|MPCB|Rv2350c|MT2415|MTCY98.19c phospholipase C 2 (512 aa), FASTA scores: opt: 2474, E(): 2.2e-142, (67.65% identity in 513 aa overlap); etc. Belongs to the bacterial phospholipase C family. P9WIB1 No intermediary metabolism and respiration P95245 P9WIB1 GO:0034480 3.1.4.3 P9WIB1 NC_000962.3 Mycobrowser_v5 CDS 2634528 2635592 . - 0 Rv2353c PPE39 Function unknown PPE family protein PPE39 Rv2353c, (MTCY98.22c), len: 354 aa. PPE39, Member of Mycobacterium tuberculosis PPE family, highly similar to many e.g. near ORF P95249|Rv2356c|MTCY98.25 from Mycobacterium tuberculosis (615 aa), FASTA scores: opt: 1566, E(): 3.2e-69, (66.1% identity in 349 aa overlap); Q10778|MTCY48.17, Q10540|MTCY31.06c, E241779|MTCY98, Q10813|MTCY274.23c, P71868|MTCY03C7.23, P71869|MTCY03C7.24c, P42611|MTV037.06C, E64997|MTCY98, Q10707|MTCY49.38C, P71657|MTCY02B10.25c, etc. Note that the ATG and RBS appear to be provided by the IR of neighbouring IS6110. Predicted to be an outer membrane protein (See Song et al., 2008). Q79FF3 No PE/PPE Q79FF3 Q79FF3 NC_000962.3 Mycobrowser_v5 CDS 2639673 2641064 . - 0 Rv2357c glyS Involved in translation mechanisms [catalytic activity: ATP + L-glycine + tRNA(GLY) = AMP + pyrophosphate + L-glycyl-tRNA(GLY)]. Probable glycyl-tRNA synthetase GlyS (glycine--tRNA ligase) (GLYRS) Rv2357c, (MTCY27.23, MTCY98.26), len: 463 aa. Probable glyS, glycyl-tRNA synthetase, equivalent to Q9CCG4|GLYS|ML0826 putative glycyl-tRNA synthase from Mycobacterium leprae (463 aa), FASTA scores: opt: 2898, E(): 1e-179, (90.2% identity in 459 aa overlap). Also highly similar to others e.g. Q9L2H9|SYG_STRCO|SCC121.07c from Streptomyces coelicolor (460 aa), FASTA scores: opt: 2210, E(): 2.9e-135, (68.3% identity in 457 aa overlap); Q9PPZ7|SYG_UREPA|GLYS|UU493 glycyl-tRNA synthetase from Ureaplasma parvum (Ureaplasma urealyticum biotype 1) (473 aa), FASTA scores: opt: 1254, E(): 1.7e-73, (45.25% identity in 462 aa overlap); P75425|SYG_MYCPN|GLYS|MPN354|MP482 glycyl-tRNA synthetase from Mycoplasma pneumoniae (449 aa), FASTA scores: opt: 1074, E(): 6.9e-62, (39.45% identity in 454 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00179 Aminoacyl-transfer RNA synthetases class-II signature 1. Belongs to class-II aminoacyl-tRNA synthetase family. P9WFV7 No information pathways P67032 P9WFV7 GO:0005737,GO:0004820,GO:0006426,GO:0046983,GO:0005524 6.1.1.14 P9WFV7 NC_000962.3 Mycobrowser_v5 CDS 2643461 2644258 . - 0 Rv2362c recO Involved in DNA repair and RECF pathway recombination Possible DNA repair protein RecO Rv2362c, (MTCY27.18), len: 265 aa. RecO, DNA repair protein, equivalent to Q9CCN0|ML0633 Mycobacterium leprae Hypothetical protein (268 aa), FASTA scores: opt: 1560, E(): 8.5e-93, (86.6% identity in 268 aa overlap). Also highly similar to others e.g. Q9L2H3|SCC121.13c DNA repair protein recO from Streptomyces coelicolor (251 aa), FASTA scores: opt: 843, E(): 6.9e-47, (52.2% identity in 249 aa overlap); and similar to other hypothetical proteins. Weak similarity with P42095|RECO_BACSU DNA repair protein recombinase from Bacillus subtilis (255 aa), FASTA scores: opt: 270, E(): 3.6e-10, (26.4% identity in 182 aa overlap). Maybe involved in modulating assembly and disassembly of RECA filaments (with RECF|Rv0003 and RECR|Rv3715c) (see citation below). Contains match to Pfam entry PF02565 Recombination protein O. Belongs to the RECO family. P9WHI5 No information pathways P65983 P9WHI5 GO:0006281,GO:0006310 P9WHI5 NC_000962.3 Mycobrowser_v5 CDS 2648916 2649974 . - 0 Rv2368c phoH1 Unknown Probable PHOH-like protein PhoH1 (phosphate starvation-inducible protein PSIH) Rv2368c, (MTCY27.12), len: 352 aa. Probable phoH1, phoH-like protein (phosphate starvation-induced protein), probably ATP-binding protein, equivalent to Q49751|PHOL_MYCLE| ML0627|B1937_F1_20 PHOH-like protein from Mycobacterium leprae (349 aa), FASTA scores: opt: 1952, E(): 4.7e-107, (88.9% identity in 352 aa overlap). Also highly similar to Q9L2L5|SCC117.05 PHOH-like protein from Streptomyces coelicolor (359 aa), FASTA scores: opt: 1407, E(): 3.6e-75, (63.6% identity in 349 aa overlap); Q9RSY1|DR1988 PHOH-related protein from Deinococcus radiodurans (380 aa), FASTA scores: opt: 1053, E(): 1.9e-54, (53.3% identity in 349 aa overlap); Q9KD58|PHOH|BH1361 phosphate starvation-induced protein from Bacillus halodurans (320 aa), FASTA scores: opt: 1019, E(): 1.6e-52, (54.35% identity in 300 aa overlap); P46343|PHOL_BACSU PHOH-like protein from Bacillus subtilis (319 aa), FASTA scores: opt: 1014, E(): 3.2e-52, (50.8% identity in 303 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the PHOH family. Note that previously known as phoH. P9WIA3 No intermediary metabolism and respiration P0A5S0 P9WIA3 GO:0003723,GO:0005737,GO:0005524 P9WIA3 NC_000962.3 Mycobrowser_v5 CDS 2652037 2652825 . - 0 Rv2372c Rv2372c Function unknown Conserved hypothetical protein Rv2372c, (MTCY27.08), len: 262 aa. Conserved hypothetical protein, equivalent to Q9CCN1|ML0626 hypothetical protein from Mycobacterium leprae (257 aa), FASTA scores: opt: 1277, E(): 3e-71, (77.25% identity in 255 aa overlap). Also highly similar to others e.g. Q9RDD9|SDRD hypothetical 26.1 KDA protein from Streptomyces coelicolor (249 aa), FASTA scores: opt: 624, E(): 3.2e-31, (45.05% identity in 253 aa overlap); P54461|YQEU_BACSU hypothetical 28.8 kDa protein from Bacillus subtilis (256 aa), FASTA scores: opt: 375, E(): 6e-16, (32.5% identity in 234 aa overlap); etc. C-terminal half highly similar to Q49763|B1937_F2_57 from Mycobacterium leprae (128 aa), FASTA scores: opt: 577, E(): 1.4e-28, (75.8% identity in 124 aa overlap). Belongs to the UPF0088 family. P9WGX1 No conserved hypotheticals P67202 P9WGX1 GO:0008168,GO:0006364,GO:0005737 2.1.1.- P9WGX1 NC_000962.3 Mycobrowser_v5 CDS 2654062 2655093 . - 0 Rv2374c hrcA Involved in transcriptional regulation (repression) of class I heat shock proteins e.g. DNAK-GRPE-DNAJ1 and groels operons). Prevents heat-shock induction of these operons. Probable heat shock protein transcriptional repressor HrcA Rv2374c, (MTCY27.06), len: 343 aa. Probable hrcA, heat-inducible transcriptional repressor (see citation below), equivalent to Q9CCN2|HRCA|ML0624 putative heat-inducible transcriptional regulator from Mycobacterium leprae (343 aa), FASTA scores: opt: 1926, E(): 3.9e-107, (89.8% identity in 343 aa overlap). Also highly similar to other heat-inducible transcription repressor proteins e.g. Q9RDD6|HRCA|SCC77.22c from Streptomyces coelicolor (338 aa), FASTA scores: opt: 1227, E(): 1.1e-65, (58.8% identity in 335 aa overlap); O52163|HRCA_STRAL from Streptomyces albus (338 aa), FASTA scores: opt: 1196, E(): 7.7e-64, (56.1% identity in 335 aa overlap); P25499|HRCA_BACSU heat-inducible transcription repressor from Bacillus subtilis (343 aa), FASTA scores: opt: 538, E(): 8.4e-25, (28.9% identity in 325 aa overlap); etc. Almost identical, but conflict at C-terminus, to Q49749|YGRP|B1937_F1_18 putative heat-inducible transcription repressor from Mycobacterium leprae (197 aa) FASTA scores: opt: 1126, E(): 6.9e-60, (91.8% identity in 195 aa overlap). Belongs to the HRCA family. P9WMK3 No virulence, detoxification, adaptation P64398 P9WMK3 GO:0045892,GO:0006950,GO:0006350,GO:0016564,GO:0003677 P9WMK3 NC_000962.3 Mycobrowser_v5 CDS 2656215 2656430 . - 0 Rv2377c mbtH Thought to be involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins. Putative conserved protein MbtH Rv2377c, (MT2445.1, MTCY27.03), len: 71 aa. Putative mbtH, conserved protein with no function assigned (see Quadri et al., 1998; De Voss et al., 1999), similar to hypothetical proteins or proteins found in several gene clusters for biosynthesis or transport of siderophores and other nonribosomally synthesized peptides e.g. Q9Z388|SCE8.11c putative small conserved hypothetical protein from Streptomyces coelicolor (71 aa), FASTA scores: opt: 345, E(): 1.4e-19, (68.2% identity in 66 aa overlap); Q9F8V3|CUMB COUY protein (probably involved in the biosynthesis of aminocoumarin antibiotic coumermycin a(1)) (see Wang et al., 2000) from Streptomyces rishiriensis (71 aa), FASTA scores: opt: 329, E(): 2.2e-18, (63.2% identity in 68 aa overlap); Q9F5J2|SIM-CB MBTH-like protein (probably protein involved in the biosynthesis of aminocoumarin antibiotic coumermycin a(1)) from Streptomyces antibioticus (70 aa), FASTA scores: opt: 308, E(): 8.4e-17, (65.6% identity in 64 aa overlap); Q9FB14 MBTH-like protein (involved in the biosynthesis of the antitumor drug bleomycin) (see Du et al., 2000) from Streptomyces verticillus FASTA scores: opt: 220, E(): 8.8e-10, (41.2% identity in 68 aa overlap); etc. P9WIP5 No lipid metabolism 2KHR O05821 P9WIP5 P9WIP5 NC_000962.3 Mycobrowser_v5 CDS 2656408 2657703 . - 0 Rv2378c mbtG Involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins. This hydroxylase is possibly required for N-hydroxylation of the two lysine residues at some stage during mycobactin assembly [catalytic activity: L-lysine + O(2) = N6-hydroxy-L-lysine + H(2)O. No information can be found if this enzyme is NADPH dependent or independent]. Lysine-N-oxygenase MbtG (L-lysine 6-monooxygenase) (lysine N6-hydroxylase) Rv2378c, (MTCY27.02), len: 431 aa. MbtG, lysine-N-oxygenase (hydroxylase) (EC 1.13.12.10 or 1.14.13.59; depending if enzyme is NADPH dependent or independent) (see citations below), showing some similarity with various proteins including ornithine and lysine-N-oxygenases, e.g. Q9K6Q1|TRKA|BH3677 potassium uptake protein from Bacillus halodurans (350 aa), FASTA scores: opt: 153, E(): 0.016, (25.2% identity in 246 aa overlap); P56584|SID1_USTMA L-ornithine 5-monooxygenase from Ustilago maydis (Smut fungus) (570 aa), FASTA scores: opt: 136, E(): 0.31, (22.85% identity in 127 aa overlap); Q9HHV0|HXYA|VNG6214G monooxygenase from Halobacterium sp. strain NRC-1 (477 aa), FASTA scores: opt: 119, E(): 3.4, (40.0% identity in 70 aa overlap); O69828|SC1A6.23 putative lysine N-hydroxlase (fragment) from Streptomyces coelicolor (134 aa), blast score: 76 (similarity in part for this one); etc. Cofactors: FAD (by similarity). P9WKF7 No lipid metabolism P9WKF7 GO:0006826,GO:0055114,GO:0047091 1.14.13.59 P9WKF7 NC_000962.3 Mycobrowser_v5 CDS 2720776 2721777 . - 0 Rv2424c Rv2424c Required for the transposition of the insertion element IS1558. Probable transposase Rv2424c, (MTCY428.23), len: 333 aa. Probable transposase for IS1558, similar to is element proteins e.g. AL021957|Rv2177c|MTV021_10 from Mycobacterium tuberculosis (221 aa), FASTA scores: opt: 1491, E(): 6.2e-87, (98.6% identity in 221 aa overlap); P19780|YIS1_STRCO hypothetical insertion element IS110 from Streptomyces coelicolor (45 aa), FASTA scores: opt: 203, E(): 1.7e-05; (27.3% identity in 238 aa overlap); etc. Contains PS01159 WW/rsp5/WWP domain signature. P71924 No insertion seqs and phages P71924 P71924 GO:0004803,GO:0006313,GO:0003677 P71924 NC_000962.3 Mycobrowser_v5 CDS 2667255 2670269 . - 0 Rv2381c mbtD Involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins. Polyketide synthetase MbtD (polyketide synthase) Rv2381c, (MTCY22H8.04), len: 1004 aa. MbtD, polyketide synthase (see citations below), similar in part to several synthases e.g. Q03132|ERY2_SACER|ERYA erythronolide synthase, modules 3 and 4 from Saccharopolyspora erythraea (Streptomyces erythraeus) (3567 aa), FASTA scores: opt: 971, E(): 1e-46, (29.35% identity in 1043 aa overlap); Q9F829|megaii megalomicin 6-deoxyerythronolide B synthase 2 from Micromonospora megalomicea subsp. nigra (3562 aa), FASTA scores: opt: 787, E(): 2.4e-36, (29.35% identity in 1032 aa overlap); Q9L4W4|NYSB polyketide synthase from Streptomyces noursei (3192 aa), FASTA scores: opt: 761, E(): 6.6e-35, (29.55% identity in 1086 aa overlap); O30764|NIDA1 polyketide synthase modules 1 and 2 from Streptomyces caelestis (4340 aa), FASTA scores: opt: 726, E(): 7.8e-33, (27.3% identity in 1052 aa overlap); etc. Contains PS00012 Phosphopantetheine attachment site. P71719 No lipid metabolism P71719 P71719 GO:0009058,GO:0048037,GO:0031177,GO:0016740,GO:0000036 P71719 NC_000962.3 Mycobrowser_v5 CDS 2983071 2983874 . + 0 Rv2666 Rv2666 Required for the transposition of the insertion element IS1081. Probable transposase for insertion sequence element IS1081 (fragment) Rv2666, (MTCY441.35), len: 267 aa. Probable transposase (fragment), identical in region of overlap to P35882|TRA1_MYCBO|TRA1_MYCTU transposase for insertion sequence element IS1081 from Mycobacterium tuberculosis or bovis (415 aa). Last 4 codons not part of gene. Contains PS01007 Transposases, Mutator family, signature. P71963 No insertion seqs and phages P71963 P71963 GO:0004803,GO:0006313,GO:0003677 P71963 NC_000962.3 Mycobrowser_v5 CDS 2677729 2678649 . + 0 Rv2385 mbtJ Involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins. Possibly required for N-hydroxylation of the two lysine residues at some stage during mycobactin assembly. Putative acetyl hydrolase MbtJ Rv2385, (MTCY253.36c), len: 306 aa. Putative mbtJ, acetyl hydrolase (see citations below), showing some similarity with various hydrolases including acetyl hydrolases e.g. Q9ZBM4|MLCB1450.08|ML0314 putative hydrolase/esterase from Mycobacterium leprae (335 aa), FASTA scores: opt: 449, E(): 6.7e-21, (33.85% identity in 313 aa overlap); AAK47950|MT3591 Esterase from M. tuberculosis strain CDC1551 (327 aa), FASTA scores: opt: 469, E(): 3.6e-22, (35% identity in 283 aa overlap); Q9X8J4|SCE9.22 putative esterase from Streptomyces coelicolor (266 aa), FASTA scores: opt: 430,E(): 8.5e-20, (38% identity in 245 aa overlap); Q01109|BAH_STRHY acetyl-hydrolase from Streptomyces hygroscopicus (299 aa), FASTA scores: opt: 420, E(): 4e-19, (35.1% identity in 265 aa overlap). Equivalent to AAK46748 from Mycobacterium tuberculosis strain CDC1551 (327 aa) but shorter 21 aa. Note that previously known as lipK. Q79FE8 No lipid metabolism Q79FE8 Q79FE8 GO:0016787 3.1.1.- Q79FE8 NC_000962.3 Mycobrowser_v5 CDS 2678653 2680005 . - 0 Rv2386c mbtI Involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins. Possibly plays a role in the conversion of chorismate to salicilate (the starter unit for mycobactin siderophore construction). Isochorismate synthase MbtI Rv2386c, (MTCY253.35), len: 450 aa. mbtI, isochorismate synthase (see citations below), similar to Q9X9I8|IRP9 salicylate synthetase from Yersinia enterocolitica (434 aa), FASTA scores: opt: 887, E(): 7.5e-48, (37.45% identity in 422 aa overlap); and similar in C-terminal region to many anthranilate synthases component I e.g. Q9Z4W7|TRPE_STRCO|SCE8.07c from Streptomyces coelicolor (511 aa), FASTA scores: opt: 509, E(): 3e-24, (40.4% identity in 255 aa overlap); P33975|TRPE_HALVO from Halobacterium volcanii (Haloferax volcanii) (523 aa) FASTA scores: opt: 488, E(): 6.2e-23, (34.2% identity in 298 aa overlap); and similar to Q08653|TRPE_THEMA|TM0142 anthranilate synthase component I from Thermotoga maritima (461 aa), FASTA scores: opt: 478, E(): 2.3e-22, (28.4% identity in 440 aa overlap); etc. Could be belong to the anthranilate synthase component I family. Note that previously known as trpE2, an anthranilate synthase component I. P9WFX1 No lipid metabolism 2G5F,2I6Y,3LOG Q7D785 P9WFX1 GO:0006826,GO:0008909,GO:0016829,GO:0046872,GO:0009058 4.1.3.-,5.4.4.2 P9WFX1 NC_000962.3 Mycobrowser_v5 CDS 2690072 2692075 . + 0 Rv2395 Rv2395 Function unknown (possibly involved in transport across the membrane). Probable conserved integral membrane protein Rv2395, (MTCY253.26c), len: 667 aa. Probable conserved integral membrane protein, similar to AAK24613|CC2646 oligopeptide transporter/opt family protein from Caulobacter crescentus (666 aa), FASTA scores: opt: 1638, E(): 4.8e-86, (51.0% identity in 658 aa overlap); Q9PIS5|CJ0204 putative integral membrane protein from Campylobacter jejuni (665 aa), FASTA scores: opt: 1484, E(): 2.9e-77, (40.6% identity in 658 aa overlap); and P44016|Y561_HAEIN hypothetical integral membrane protein from Haemophilus influenzae (635 aa), FASTA scores: opt: 1449, E(): 2.8e-75, (42.15% identity in 624 aa overlap). P71749 No cell wall and cell processes P71749 P71749 GO:0055085 P71749 NC_000962.3 Mycobrowser_v5 CDS 2696644 2697714 . - 0 Rv2400c subI Involved in the active transport across the membrane of multiple sulfur-containing compounds, including sulfate and thiosulfate (import). Probable sulfate-binding lipoprotein SubI Rv2400c, (MTCY253.21), len: 356 aa. Probable subI, sulfate-binding lipoprotein component of sulfate transport system (see citations below), equivalent to Q9CCN3|SUBI|ML0615 (alias Q49748|B1937_F1_11, 358 aa) putative sulphate-binding protein from Mycobacterium leprae (348 aa), FASTA scores: opt: 1775, E(): 2.3e-102, (76.45% identity in 340 aa overlap). Also similar to others and other substrate-binding proteins e.g. P27366|SUBI_SYNP7|SBPA sulfate-binding protein precursor from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (350 aa), FASTA scores: opt: 703, E(): 4.6e-36, (35.6% identity in 351 aa overlap); Q9I6K7|SBP|PA0283 sulfate-binding protein precursor from Pseudomonas aeruginosa (332 aa), FASTA scores: opt: 591, E(): 3.7e-29, (36.9% identity in 317 aa overlap); CAC49112|SMB21133 putative sulfate uptake ABC transporter periplasmic solute-binding protein precursor from Rhizobium meliloti (Sinorhizobium meliloti) (341 aa), FASTA scores: opt: 569, E(): 8.8e-28, (36.15% identity in 321 aa overlap); etc. Belongs to the prokaryotic sulfate binding protein family. P71744 No cell wall and cell processes P71744 GO:0015419,GO:0008272,GO:0030288 P71744 NC_000962.3 Mycobrowser_v5 CDS 2701287 2703248 . - 0 Rv2404c lepA Involved in translation. Probable GTP-binding protein LepA (GTP-binding elongation factor) Rv2404c, (MT2476, MTCY253.16), len: 653 aa. Probable lepA, GTP-binding protein (a protein of unknown function, but apparently with membrane-related functions and very similar to protein synthesis elongation factors; see citations below). Equivalent to P53530|LEPA_MYCLE|ML0611|B1937_F3_81 GTP-binding protein from Mycobacterium leprae (646 aa), FASTA scores: opt: 3610, E(): 1.2e-205, (88.0% identity in 649 aa overlap). Also highly similar to many GTP-binding proteins LEPA e.g. Q9RDC9|LEPA_STRCO|SCC77.29c from Streptomyces coelicolor (622 aa), FASTA scores: opt: 3046, E(): 2.3e-172, (74.3% identity in 626 aa overlap); P37949|LEPA_BACSU from B. subtilis (612 aa), FASTA scores: opt: 2430, E(): 5.3e-136, (58.7% identity in 610 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00301 GTP-binding elongation factors signature. Belongs to the GTP-binding elongation factor family, LEPA subfamily. P9WK97 No intermediary metabolism and respiration P65269 P9WK97 GO:0003924,GO:0005525 P9WK97 NC_000962.3 Mycobrowser_v5 CDS 2721866 2723308 . - 0 Rv2425c Rv2425c Function unknown Conserved hypothetical protein Rv2425c, (MTCY428.22), len: 480 aa. Hypothetical protein; C-terminal half shares similarity to other unknown conserved proteins e.g. Q53065 hypothetical 24.3 KDA protein from Rhodococcus erythropolis (219 aa), FASTA scores: opt: 398, E(): 9.9e-17, (34.15% identity in 202 aa overlap); C-terminus of O27843|MTH1815 conserved protein from Methanothermobacter thermautotrophicus (346 aa), FASTA scores: opt: 341, E(): 3.7e-13, (31.35% identity in 233 aa overlap); etc. P71923 No conserved hypotheticals P71923 P71923 P71923 NC_000962.3 Mycobrowser_v5 CDS 2706494 2707333 . - 0 Rv2409c Rv2409c Function unknown Conserved protein Rv2409c, (MTCY253.11), len: 279 aa. Conserved protein, equivalent to Q49757|YP69_MYCLE|G466976|B1937_F2_39 hypothetical protein from Mycobacterium leprae (279 aa), FASTA scores: opt: 1564, E(): 4.6e-95, (82.1% identity in 279 aa overlap). Also similar to others e.g. Q9RSX6|DR1993 from Deinococcus radiodurans (274 aa), FASTA scores: opt: 494, E(): 4e-25, (35.1% identity in 282 aa overlap); BAB49898|Mll2875 from Rhizobium loti (Mesorhizobium loti) (294 aa), FASTA scores: opt: 382, E(): 8.9e-18, (29.75% identity in 269 aa overlap); Q9I305|PA1732 from Pseudomonas aeruginosa (266 aa), FASTA scores: opt: 326, E(): 3.7e-14, (31.25% identity in 275 aa overlap); etc. Also similar to Rv2569c|MTCY227.32 from Mycobacterium tuberculosis. P71734 No conserved hypotheticals P71734 P71734 P71734 NC_000962.3 Mycobrowser_v5 CDS 2708310 2709965 . - 0 Rv2411c Rv2411c Function unknown Conserved hypothetical protein Rv2411c, (MTCY253.09c), len: 551 aa. Hypothetical protein, highly similar to Q49755|YO11_MYCLE|ML0605|MLCL536.05c|U1937B|B1937_F1_4 hypothetical 61.8 KDA protein from Mycobacterium leprae (561 aa), FASTA scores, opt: 3163, E(): 4.1e-178, (87.35% identity in 554 aa overlap). Also highly similar, except in N-terminus, to others e.g. Q55587|Y335_SYNY3|SLL0335 hypothetical protein from Synechocystis sp. strain PCC 6803 (481 aa), FASTA scores: opt: 1620, E(): 1.2e-87, (52.8% identity in 468 aa overlap); Q9I307|PA1730 hypothetical protein from Pseudomonas aeruginosa (470 aa), FASTA scores: opt: 1574, E(): 5.8e-85, (52.7% identity in 467 aa overlap); Q9RST1|DR2042 conserved hypothetical protein from Deinococcus radiodurans (655 aa), FASTA scores: opt: 1561, E(): 4.4e-84, (53.3% identity in 467 aa overlap); etc. P9WLA9 No conserved hypotheticals P65001 P9WLA9 P9WLA9 NC_000962.3 Mycobrowser_v5 CDS 2712891 2713784 . - 0 Rv2415c Rv2415c Function unknown Conserved hypothetical protein Rv2415c, (MTCY253.05), len: 297 aa. Hypothetical protein, with some similarity in C-terminal part to comE operon proteins 1 e.g. Q9EU10|come|COME4|COME1|COME2|COME3 come protein (a competence protein with DNA-binding activity) from Neisseria gonorrhoeae (99 aa), FASTA scores: opt: 190, E(): 0.0032, (49.2% identity in 61 aa overlap); Q9JYB8|NMB1657 from Neisseria meningitidis (205 aa) FASTA scores: opt: 191, E(): 0.0052, (49.2% identity in 61 aa overlap); CME1_BACSU|P39694 come operon protein 1 from Bacillus subtilis (205 aa), FASTA scores, opt: 181, E(): 0.017 (29.8% identity in 218 aa overlap); etc. P71728 No conserved hypotheticals P71728 P71728 GO:0006281,GO:0003677 P71728 NC_000962.3 Mycobrowser_v5 CDS 2724230 2725477 . - 0 Rv2427c proA Involved in proline biosynthesis pathway (at the second step). Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. [catalytic activity: L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-gamma-glutamyl 5-phosphate + NADPH]. Probable gamma-glutamyl phosphate reductase protein ProA (GPR) (glutamate-5-semialdehyde dehydrogenase) (glutamyl-gamma-semialdehyde dehydrogenase) Rv2427c, (MTCY428.20), len: 415 aa. Probable proA, gamma-glutamyl phosphate reductase protein, equivalent to Q9CBZ7|ML1458|PROA [gamma]-glutamyl phosphate reductase from Mycobacterium leprae (409 aa), FASTA scores: opt: 2120, E(): 7.4e-118, (81.9% identity in 409 aa overlap). Also highly similar or similar to other gamma-glutamyl phosphate reductases proteins (GPR) e.g. Q9RDK1|PROA from Streptomyces coelicolor (428 aa), FASTA scores: opt: 1073, E(): 4.6e-56, (60.4% identity in 429 aa overlap); P45638|PROA_CORGL from Corynebacterium glutamicum (432 aa), FASTA scores: opt: 993, E(): 2.4e-51, (58.5% identity in 417 aa overlap); P96489|PROA_STRTR gamma-glutamyl phosphate reductase from Streptococcus thermophilus (416 aa), FASTA scores: opt: 863, E(): 1.1e-43, (49.15% identity in 413 aa overlap); etc. Belongs to the gamma-glutamyl phosphate reductase family. P9WHV1 No intermediary metabolism and respiration P65788 P9WHV1 GO:0005737,GO:0004350,GO:0055114,GO:0006561,GO:0050661 1.2.1.41 P9WHV1 NC_000962.3 Mycobrowser_v5 CDS 2726806 2727339 . + 0 Rv2429 ahpD Involved in oxidative stress response. LPDC|Rv0462, DLAT|Rv2215, AHPD|Rv2429, and AHPC|Rv2428 constitute an NADH-dependent peroxidase and peroxynitrite reductase that provides protection against oxidative stress. Alkyl hydroperoxide reductase D protein AhpD (alkyl hydroperoxidase D) Rv2429, (MTCY428.17c), len: 177 aa. AhpD, alkyl hydroperoxide reductase, similar to other alkyl hydroperoxide reductases D proteins e.g. Q9RN73|AHPD from Streptomyces coelicolor (178 aa), FASTA scores: opt: 611, E(): 1.4e-33, (57.4% identity in 169 aa overlap); Q50441|AHPD_MYCSM AHPD protein (fragment) from Mycobacterium smegmatis (52 aa), FASTA score: opt:196. P9WQB5 No virulence, detoxification, adaptation 1GU9,1KNC,1LW1,1ME5 P0A5N4 P9WQB5 GO:0055114,GO:0004601,GO:0051920,GO:0008785 1.11.1.15 P9WQB5 NC_000962.3 Mycobrowser_v5 CDS 2728844 2729134 . - 0 Rv2433c Rv2433c Unknown Hypothetical protein Rv2433c, (MTCY428.13), len: 96 aa. Hypothetical unknown protein. P71916 No conserved hypotheticals P71916 NC_000962.3 Mycobrowser_v5 CDS 2729115 2730560 . - 0 Rv2434c Rv2434c Unknown Probable conserved transmembrane protein Rv2434c, (MTCY428.12), len: 481 aa. Probable conserved transmembrane protein, with some similarity to BAB48444|MLR0973 probable integral membrane protein from Rhizobium loti (410 aa), FASTA scores: opt: 298, E(): 4.1e-11, (27.25% identity in 389 aa overlap); and also similarity with other hypothetical proteins and/or putative integral membrane proteins. P71915 No cell wall and cell processes P71915 P71915 GO:0055085,GO:0016021 P71915 NC_000962.3 Mycobrowser_v5 CDS 2733230 2734144 . + 0 Rv2436 rbsK Involved in ribose metabolism (in the first step) [catalytic activity: ATP + D-ribose = ADP + D-ribose 5-phosphate]. Ribokinase RbsK Rv2436, (MTCY428.10c), len: 304 aa. Probable rbsK, ribokinase, similar to others e.g. Q9RZ99|DRA0055 from Deinococcus radiodurans (300 aa) FASTA scores: opt: 485, E(): 9.1e-21, (44.55% identity in 301 aa overlap); P36945|P96733|RBSK_BACSU from Bacillus subtilis (293 aa), FASTA scores: opt: 398, E(): 8.5e-16, (36.35% identity in 297 aa overlap); P05054|RBSK_ECOLI|B3752|Z5253|ECS4694 from Escherichia coli strain K12 (309 aa), FASTA scores: opt: 387, E(): 3.8e-15, (34.7% identity in 314 aa overlap); etc. Contains PS00583 pfkB family of carbohydrate kinases signature 1. Belongs to the PFKB family of carbohydrate kinases. P71913 No intermediary metabolism and respiration 3GO6,3GO7 P71913 P71913 GO:0004747,GO:0006014 2.7.1.15 P71913 NC_000962.3 Mycobrowser_v5 CDS 2734792 2736831 . - 0 Rv2438c nadE Involved in biosynthesis of NAD. Can use both glutamine or ammonia as a nitrogen source [catalytic activity: ATP + deamido-NAD(+) + L-glutamine + H(2)O = AMP + diphosphate + NAD(+) + L-glutamate]. Glutamine-dependent NAD(+) synthetase NadE (NAD(+) synthase [glutamine-hydrolysing]) Rv2438c, (MT2513, MTCY428.08), len: 679 aa. NadE, glutamine-dependent NAD(+) synthetase (see citation below), equivalent to Q9CBZ6|NADE_MYCLE|ML1463 Glutamine-dependent NAD(+) synthetase from Mycobacterium leprae (680 aa), FASTA scores: opt: 3877, E(): 0. Also similar to others e.g. O83759|NADE_TREPA|TP0780 from Treponema pallidum (679 aa), FASTA scores: opt: 543, E(): 1.1e-25; O74940|NADE_SCHPO|SPCC553.02 from Schizosaccharomyces pombe (Fission yeast) (700 aa), FASTA scores: opt: 354, E(): 4.7e-14 ; P38795|NADE_YEAST|YHR074W from Saccharomyces cerevisiae (Baker's yeast) (714 aa), FASTA scores: opt: 339, E(): 4e-13; etc. Contains PS00591 Glycosyl hydrolases family 10 active site. Belongs to the NAD synthetase family in the C-terminal section. N-terminus shorter since first submission. P9WJJ3 No intermediary metabolism and respiration 3DLA P0A5L6 P9WJJ3 GO:0009435,GO:0003952,GO:0016810,GO:0005524 6.3.5.1 P9WJJ3 NC_000962.3 Mycobrowser_v5 CDS 2737117 2738247 . - 0 Rv2439c proB Involved in proline biosynthesis pathway (at the first step). Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline [catalytic activity: ATP + L-glutamate = ADP + L-glutamate 5- phosphate]. Probable glutamate 5-kinase protein ProB (gamma-glutamyl kinase) (GK) Rv2439c, (MTCY428.07), len: 376 aa. Probable proB, glutamate 5-kinase protein (GK), equivalent to Q9CBZ5|prob|ML1464 from Mycobacterium leprae (367 aa) FASTA scores: opt: 1937, E(): 1.1e-102, (84.4% identity in 366 aa overlap). Also highly similar to other glutamate 5-kinase proteins e.g. P46546|PROB_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (369 aa), FASTA scores: opt: 1241, E(): 3e-63, (54.35% identity in 368 aa overlap); Q9ZG98|PROB_MEIRU glutamate 5-kinase from Meiothermus ruber (390 aa), FASTA scores: opt: 825, E(): 1.2e-39, (45.05% identity in 353 aa overlap); Q9RDJ9|prob|SCC123.25c from Streptomyces coelicolor (374 aa), FASTA scores: opt: 1193, E(): 1.6e-60, (55.85% identity in 367 aa overlap); etc. Contains PS00902 Glutamate 5-kinase signature. Belongs to the glutamate 5-kinase family. P9WHU9 No intermediary metabolism and respiration P71910 P9WHU9 GO:0003723,GO:0005737,GO:0004349,GO:0006561,GO:0005524 2.7.2.11 P9WHU9 NC_000962.3 Mycobrowser_v5 CDS 2752262 2752660 . + 0 Rv2451 Rv2451 Unknown Hypothetical proline and serine rich protein Rv2451, (MTV008.07), len: 132 aa. Hypothetical unknown pro-, ser-rich protein. O53178 No conserved hypotheticals O53178 O53178 NC_000962.3 Mycobrowser_v5 CDS 2738247 2739686 . - 0 Rv2440c obg Essential GTP-binding protein. Probable GTP1/Obg-family GTP-binding protein Obg Rv2440c, (MTCY428.06), len: 479 aa. Probable obg, nucleotide-binding protein, equivalent to Q9CBZ4|ML1465 GTP1/OBG-family GTP-binding protein from Mycobacterium leprae (478 aa), FASTA scores: opt: 1328, E(): 8.4e-70, (58.9% identity in 479 aa overlap). Also highly similar to others e.g. P95722|OBG GTP-binding protein from Streptomyces coelicolor (478 aa), FASTA scores: opt: 1311, E(): 8.2e-69, (60.7% identity in 476 aa overlap); P20964|OBG_BACSU SPO0B-associated GTP-binding protein from Bacillus subtilis (428 aa), FASTA scores: opt: 1006, E(): 3.9e-51, (42.9% identity in 436 aa overlap); Q9KDK0|OBG|BH1213 GTP-binding protein involved in initiation of sporulation from Bacillus halodurans (427 aa), FASTA scores: opt: 978, E(): 1.7e-49, (41.95% identity in 436 aa overlap); etc. Highly similar (identical but shorter 5 aa) to AAK46813|MT2516 GTP-binding protein from Mycobacterium tuberculosis strain CDC1551 (484 aa), FASTA scores: opt: 3205, E(): 7.9e-179, (100% identity in 479 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the GTP1/OBG family. P9WMT1 No intermediary metabolism and respiration P71909 P9WMT1 GO:0003924,GO:0005737,GO:0005525 P9WMT1 NC_000962.3 Mycobrowser_v5 CDS 2740709 2742184 . + 0 Rv2443 dctA Involved in the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the inner membrane. Responsible for the translocation of the substrate across the membrane. Probable C4-dicarboxylate-transport transmembrane protein DctA Rv2443, (MTCY428.03c), len: 491 aa. Probable dctA, C4-dicarboxylate-transport transmembrane protein, similar to other C4-dicarboxylate transport proteins e.g. AAK46817|MT2519 from Mycobacterium tuberculosis strain CDC1551 (491 aa); Q9L1K8|SC6A11.12 putative sodium:dicarboxylate symporter from Streptomyces coelicolor (466 aa), FASTA scores: opt: 1797, E(): 2.9e-98, (61.3% identity in 452 aa overlap); Q9RRG7|DR2525 from Deinococcus radiodurans (463 aa); P50334|DCTA_SALTY from Salmonella typhimurium (428 aa) FASTA scores: opt: 1241, E(): 1.3e-65, (47.2% identity in 415 aa overlap); etc. Belongs to the sodium dicarboxylate symporter family (SDF) (DAACS family). P71906 No cell wall and cell processes P71906 P71906 GO:0016021,GO:0017153,GO:0006835 P71906 NC_000962.3 Mycobrowser_v5 CDS 2746135 2747598 . - 0 Rv2447c folC Conversion of folates to polyglutamate derivatives. Bacteria require folate for the biosynthesis of glycine, methionine, formyl-met-tRNA, thymidylates, purines, and pantothenate [catalytic activity: ATP + {tetrahydrofolyl-[GLU]}(N) + L-glutamate = ADP + phosphate + {tetrahydrofolyl-[GLU]}(N+1)]. Probable folylpolyglutamate synthase protein FolC (folylpoly-gamma-glutamate synthetase) (FPGS) Rv2447c, (MTV008.03c), len: 487 aa. Probable folC, folylpolyglutamate synthase, equivalent to Q9CBY8|FOLC|ML1471 from Mycobacterium leprae (485 aa), FASTA scores: opt: 2425, E(): 2.2e-134, (78.7% identity in 483 aa overlap). Also highly similar to others e.g. Q9L1G4|FPGS|O08416|Y13070 from Streptomyces coelicolor (444 aa), FASTA scores: opt: 774, E(): 6.3e-38, (53.9% identity in 462 aa overlap); P15925|FOLC_LACCA|FGS from Lactobacillus casei (428 aa), FASTA scores: opt: 631, E(): 1.4e-29, (34.55% identity in 437 aa overlap); Q05865|FOLC_BACSU from Bacillus subtilis (430 aa), FASTA scores: opt: 421, E(): 2.6e-17, (32.9% identity in 383 aa overlap); etc. Contains PS01012 Folylpolyglutamate synthase signature 2. Belongs to the folylpolyglutamate synthase family. I6Y0R5 No intermediary metabolism and respiration 2VOR,2VOS O53174 I6Y0R5 GO:0009396,GO:0004326,GO:0005524 6.3.2.17 I6Y0R5 NC_000962.3 Mycobrowser_v5 CDS 2747595 2750225 . - 0 Rv2448c valS Involved in translation mechanisms [catalyic activity: ATP + L-valine + tRNA(val) = AMP + diphosphate + L-valyl-tRNA(val)]. Probable valyl-tRNA synthase protein ValS (valyl-tRNA synthetase) (valine--tRNA ligase) (valine translase) Rv2448c, (MTV008.04c), len: 876 aa. Probable valS, valyl-tRNA synthetases, equivalent to Q9CBY7|VALS|ML1472 valyl-tRNA synthase from Mycobacterium leprae (886 aa), FASTA scores: opt: 5181,E(): 0, (85.4% identity in 876 aa overlap). Also highly similar to others e.g. O06851|SYV_STRCO from Streptomyces coelicolor (874 aa), FASTA scores: opt: 2470, E(): 1.6e-143, (60.45% identity in 880 aa overlap); Q9X2D7|SYV_THEMA|VALS|TM1817 from Thermotoga maritima (865 aa), FASTA scores: opt: 2418, E(): 2.4e-140, (44.2% identity in 891 aa overlap); Q05873|SYV_BACSU|VALS from Bacillus subtilis (880 aa), FASTA scores: opt: 2063, E(): 1.4e-118, (46.08% identity in 894 aa overlap); etc. Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Contains probable coiled-coil from aa 810 to 846. Belongs to class-I aminoacyl-tRNA synthetase family. P9WFS9 No information pathways P67599 P9WFS9 GO:0005737,GO:0004832,GO:0006438,GO:0005524 6.1.1.9 P9WFS9 NC_000962.3 Mycobrowser_v5 CDS 2751662 2752180 . - 0 Rv2450c rpfE Thought to promote the resuscitation and growth of dormant, nongrowing cells. Could also stimulate the growth of several other high G+C gram+ organisms, e.g. Mycobacterium avium, Mycobacterium bovis (BCG), Mycobacterium kansasii, Mycobacterium smegmatis. Probable resuscitation-promoting factor RpfE Rv2450c, (MTV008.06c), len: 172 aa. Probable rpfE, resuscitation-promoting factor (see Mukamolova et al., 1998), similar to O86308|Z96935|MLRPF_1 RPF protein precursor from Micrococcus luteus (220 aa), FASTA scores: opt: 291, E(): 3e-7, (48.75% identity in 80 aa overlap). C-terminus is similar to other Mycobacterial rpf proteins e.g. O05594|Rv1009|MTCI237.26|RPFB probable resuscitation-promoting factor from Mycobacterium tuberculosis (362 aa), FASTA scores: opt: 344, E(): 1.4e-09, (42.85% identity in 147 aa overlap); etc. C-terminal region similar to N-terminal region of Q9F2Q2|SCE41.06c putative secreted protein from Streptomyces coelicolor (244 aa), FASTA scores: opt: 355, E(): 3.1e-10, (56.65% identity in 90 aa overlap). Also similar to Q9F2Q1|SCE41.07c putative secreted protein from Streptomyces coelicolor (near Q9F2Q2|SCE41.06c) (341 aa) FASTA scores: opt: 317, E(): 2.5e-08, (51.7% identity in 87 aa overlap). With Mycobacterium leprae, high similarity between the two corresponding C-terminal regions of two hypothetical proteins, Q9CD53|ML0240 (375 aa), FASTA scores: opt: 339, E(): 2.5e-09, (59.15% identity in 93 aa overlap) and O33049|MLCB57.05c|ML2151 (174 aa), FASTA scores: opt: 329, E(): 4e-09, (58.14% identity in 86 aa overlap). Contains a possible secretory signal sequence in N-terminus. Possible autocrine and/or paracrine bacterial growth factor or cytokine (see citations below). Interacts with RipA (see Hett et al., 2007). Predicted possible vaccine candidate (See Zvi et al., 2008). O53177 No cell wall and cell processes O53177 O53177 GO:0005576 O53177 NC_000962.3 Mycobrowser_v5 CDS 2758208 2759488 . - 0 Rv2457c clpX ATP-dependent specificity component of the CLP protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of CLPP). Probable ATP-dependent CLP protease ATP-binding subunit ClpX Rv2457c, (MTV008.13c), len: 426 aa. Probable clpX, ATP-dependent clp protease ATP-binding subunit clpX, equivalent to Q9CBY6|CLPX|ML1477 ATP-dependent CLP protease ATP-binding protein from Mycobacterium leprae (426 aa), FASTA scores: opt: 2652, E(): 1.4e-142, (96.0% identity in 426 aa overlap). Also highly similar to others e.g. Q9F316|CLPX from Streptomyces coelicolor (428 aa) FASTA scores: opt: 2178, E(): 8.2e-116, (77.8% identity in 428 aa overlap); P50866|CLPX_BACSU from Bacillus subtilis (420 aa), FASTA scores: opt: 1788, E(): 8.5e-94, (63.6% identity in 426 aa overlap); P33138|CLPX_ECOLI from Escherichia coli (423 aa), FASTA scores: opt: 1694, E(): 1.7e-88, (62.4% identity in 415 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the CLPX chaperone family. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P9WPB9 No intermediary metabolism and respiration P0A528 P9WPB9 GO:0016887,GO:0046983,GO:0006457,GO:0051082,GO:0008270,GO:0005524 P9WPB9 NC_000962.3 Mycobrowser_v5 CDS 2763172 2763774 . - 0 Rv2461c clpP1 CLP cleaves peptides in various proteins in a process that requires ATP hydrolysis. CLP may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. Probable ATP-dependent CLP protease proteolytic subunit 1 ClpP1 (endopeptidase CLP) Rv2461c, (MT2536, MTV008.17c), len: 200 aa. Probable clpP1, ATP-dependent clp protease proteolytic subunit 1, equivalent to Q9CBY3|CLP1_MYCLE ATP-dependent CLP protease proteolytic subunit from Mycobacterium leprae (224 aa), FASTA scores: opt: 1226, E(): 1.3e-71, (95.0% identity in 200 aa overlap). Also highly similar to others e.g. Q9F315|CLPP1 from Streptomyces coelicolor (219 aa), FASTA scores: opt: 713, E(): 9.3e-39, (61.75% identity in 183 aa overlap); P80244|CLPP_BACSU from Bacillus subtilis (197 aa), FASTA scores: opt: 658, E(): 2.8e-35, (54% identity in 187 aa overlap); Q9WZF9|CLPP_THEMA|TM0695 from Thermotoga maritima (203 aa), FASTA scores: opt: 653, E(): 6.1e-35, (55.25% identity in 172 aa overlap); etc. Also similar to downstream ORF Rv2460c|MTV008.16c|clpP2 (214 aa), FASTA score: (48.3% identity in 172 aa overlap). Belongs to peptidase family S14, also known as CLPP family. Note that previously known as clp. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P9WPC5 No intermediary metabolism and respiration 2C8T,2CBY,2CE3 P0A526 P9WPC5 GO:0005737,GO:0006508,GO:0004252,GO:0005524 3.4.21.92 P9WPC5 NC_000962.3 Mycobrowser_v5 CDS 2924817 2925383 . - 0 Rv2594c ruvC Nuclease that resolves holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous ARMS and leaves a 5'terminal phosphate and a 3'terminal hydroxyl group [catalytic activity: endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (holliday junction)]. Probable crossover junction endodeoxyribonuclease RuvC (holliday junction nuclease) (holliday junction resolvase) Rv2594c, (MTCY227.07), len: 188 aa. Probable ruvC, Holliday junction resolvase (see citations below), equivalent to P40834|RUVC_MYCLE|ML0481|B1177_C3_226 crossover junction endodeoxyribonuclease from Mycobacterium leprae (188 aa), FASTA scores: opt: 984, E(): 2.3e-55, (81.0% identity in 184 aa overlap). Also highly similar to others e.g. Q9AE11|RUVC from Corynebacterium glutamicum (Brevibacterium flavum) (221 aa), FASTA scores: opt: 713, E(): 3.6e-38, (56.9% identity in 188 aa overlap); Q9L289|RUVC_STRCO|SCL2.10c from Streptomyces coelicolor (188 aa), FASTA scores: opt: 704, E(): 1.2e-37, (60.65% identity in 178 aa overlap); P24239|RUVC_ECOLI|B1863 from Escherichia coli strain K12 (172 aa), FASTA scores: opt: 322, E(): 1.6e-13, (38.65% identity in 163 aa overlap); etc. Belongs to the RUVC family. Cofactor: magnesium. P9WGV9 No information pathways P66760 P9WGV9 GO:0006281,GO:0008821,GO:0000287,GO:0003676,GO:0006310 3.1.22.4 P9WGV9 NC_000962.3 Mycobrowser_v5 CDS 2765655 2766839 . + 0 Rv2463 lipP Function unknown; lipolytic enzyme involved in cellular metabolism. Probable esterase/lipase LipP Rv2463, (MTV008.19), len: 394 aa. Probable lipP, esterase, lipase similar to others eg O87861|ESTA esterase a from Streptomyces chrysomallus (389 aa), FASTA scores: opt: 964, E(): 1.9e-53, (44.35% identity in 399 aa overlap); Q9I4S7|PA1047 probable esterase from Pseudomonas aeruginosa (392 aa), FASTA scores: opt: 863, E(): 4.6e-47, (40.05% identity in 377 aa overlap); Q53403|ESTC esterase III from Pseudomonas fluorescens (382 aa), FASTA scores: opt: 753, E(): 3.9e-40, (36.3% identity in 380 aa overlap); etc. O53190 No intermediary metabolism and respiration O53190 O53190 GO:0016787 3.1.-.- O53190 NC_000962.3 Mycobrowser_v5 CDS 2767671 2768159 . - 0 Rv2465c rpiB Interconverts ribose-5-phosphate and ribulose-5-phosphate Ribose-5-phosphate isomerase Rv2465c, (MTV008.21c), len: 162 aa. RpiB, Ribose-5-phosphate isomerase, proven biochemically (see Roos et al., 2004) equivalent to AAK46840|MT2540 putative carbohydrate-phosphate isomerase from Mycobacterium tuberculosis strain CDC1551 (159 aa). Equivalent to Q9CBY1|ML1484 possible phosphopentose isomerase from Mycobacterium leprae (162 aa), FASTA scores: opt: 992, E(): 7.1e-59, (89.5% identity in 162 aa overlap). Also highly similar or similar to several diverse isomerases e.g. Q9L206|SC8E4.02c putative isomerase from Streptomyces coelicolor (159 aa), FASTA scores: opt: 661, E(): 6.1e-37, (61.45% identity in 153 aa overlap); P47636|Y396_MYCGE|MG396 hypothetical LACA/RPIB family protein from Mycoplasma genitalium (152 aa), FASTA scores: opt: 357, E(): 8.2e-17, (42% identity in 150 aa overlap); P53527|Y396_MYCPN|MPN595|MP247 hypothetical LACA/RPIB family protein from Mycoplasma pneumoniae (152 aa), FASTA scores: opt: 340, E(): 1.1e-15, (38.6% identity in 145 aa overlap); P26592|LACB_STAAU galactose-6-phosphate isomerase from Staphylococcus aureus (171 aa), FASTA scores: opt: 296, E(): 1e-12, (35.4% identity in 158 aa overlap) and P37351|RPIB_ECOLI ribose 5-phosphate isomerase b from Escherichia coli (149 aa), FASTA scores: opt: 262, E(): 1.6e-10, (32.2% identity in 146 aa overlap); etc. Could belong to the LACA/RPIB family. P9WKD7 No intermediary metabolism and respiration 1USL,2BES,2BET,2VVO,2VVP,2VVQ Q79FD7 P9WKD7 GO:0004751,GO:0005975 5.3.1.6 P9WKD7 NC_000962.3 Mycobrowser_v5 CDS 2772367 2773035 . - 0 Rv2469c Rv2469c Function unknown Conserved hypothetical protein Rv2469c, (MTV008.25c), len: 222 aa. Conserved hypothetical protein, highly similar to other hypothetical proteins e.g. Q9X7B4|MLCB1610.15|ML1254 from Mycobacterium leprae (215 aa), FASTA scores: opt: 1183, E(): 3.3e-70, (77.9% identity in 222 aa overlap); Q9L1Y0|SC8E4A.25c from Streptomyces coelicolor (178 aa), FASTA scores: opt: 589, E(): 1.7e-31, (53.4% identity in 161 aa overlap) (N-terminal region is shorter 50 aa approximately); Q9RRS6|DR2409 conserved hypothetical protein from Deinococcus radiodurans (186 aa), FASTA scores: opt: 440, E(): 9.6e-22, (42.25% identity in 168 aa overlap) (N-terminal region is shorter 30 aa approximately); etc. O53196 No conserved hypotheticals O53196 O53196 GO:0003676,GO:0004519 O53196 NC_000962.3 Mycobrowser_v5 CDS 2773564 2775204 . + 0 Rv2471 aglA Involved in sugar metabolism (hydrolysis of terminal, non-reducing 1,4-linked D-glucose residues with release of D-glucose). Probable alpha-glucosidase AglA (maltase) (glucoinvertase) (glucosidosucrase) (maltase-glucoamylase) (lysosomal alpha-glucosidase) (acid maltase) Rv2471, (MTV008.27), len: 546 aa. Probable aglA, maltase (alpha-glucosidase), highly similar or similar to several e.g. Q60027|AGLA from Thermomonospora curvata (544 aa), FASTA scores: opt: 2071, E(): 4e-116, (57.7% identity in 525 aa overlap); Q9KZE3|AGLAE from Streptomyces coelicolor (534 aa), FASTA scores: opt: 1475, E(): 1.5e-80, (50.1% identity in 537 aa overlap); O86874|AGLA from Streptomyces lividans (534 aa), FASTA scores: opt: 1473, E(): 2e-80, (50.1% identity in 537 aa overlap); etc. Seems to belong to family 13 of glycosyl hydrolases, also known as the alpha-amylase family. O53198 No intermediary metabolism and respiration O53198 O53198 GO:0005975,GO:0043169,GO:0004558 3.2.1.20 O53198 NC_000962.3 Mycobrowser_v5 CDS 2823256 2824593 . - 0 Rv2508c Rv2508c Unknown Probable conserved integral membrane leucine and alanine rich protein Rv2508c, (MTCY07A7.14c), len: 445 aa. Probable conserved integral membrane leu-, ala-rich protein, equivalent to Q9CCU4|ML0430 putative membrane protein from Mycobacterium leprae (454 aa) (alias O07710|MLCL383.37 longer 10 aa), FASTA scores: opt: 2205, E(): 2.5e-124, (75.75% identity in 441 aa overlap). Also similar to hypothetical or membrane proteins e.g. BAB50841|MLL4103 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (458 aa), FASTA scores: opt: 396, E(): 2.4e-16, (27.75% identity in 447 aa overlap); Q9RKX9|SC6D7.19c putative integral membrane protein from Streptomyces coelicolor (486 aa), FASTA scores: opt: 323, E(): 5.7e-12, (28.95% identity in 428 aa overlap); P42306|YXIO_BACSU probable integral membrane protein from Bacillus subtilis (428 aa), FASTA scores: opt: 220, E(): 7.2e-06, (20.35% identity in 413 aa overlap); etc. Also similar to proteins from Mycobacterium tuberculosis e.g. Q10564|Y876_MYCTU|Rv0876c|MT0899|MTCY31.04c (548 aa), FASTA scores: opt: 184, E(): 0.0012, (24.7% identity in 466 aa overlap). O06171 No cell wall and cell processes O06171 NC_000962.3 Mycobrowser_v5 CDS 2777388 2782262 . - 0 Rv2476c gdh Catabolic glutdh involved in the utilization of glutamate and other amino acids of the glutamate family. Generates 2-oxoglutarate from L-glutamate [catalytic activity: L-glutamate + H(2)O + NAD(+) = 2-oxoglutarate + NH(3) + NADH]. Probable NAD-dependent glutamate dehydrogenase Gdh (NAD-Gdh) (NAD-dependent glutamic dehydrogenase) Rv2476c, (MTV008.32c), len: 1624 aa. Probable gdh, glutamate dehydrogenase. Highly similar to Q9X7B2|MLCB1610.10|ML1249 hypothetical 177.9 KDA protein from Mycobacterium leprae (1622 aa), FASTA scores: opt: 8630,E(): 0, (81.45% identity in 1634 aa overlap). But highly similar to Q9F0J1|GDH NAD-glutamate dehydrogenase from Streptomyces clavuligerus (1651 aa), FASTA scores: opt: 3833, E(): 0, (45.8% identity in 1600 aa overlap); (see Minambres et al., 2000). Also similar with others e.g. AAG53963|PA3068|GDHB hypothetical (NAD(+)-dependent glutamate dehydrogenase from Pseudomonas aeruginosa (1620 aa), FASTA scores: opt: 2214, E(): 1e-124, (40.1% identity in 1561 aa overlap) (see Lu & Abdelal 2001); and Q9Y8G5|GDHB NAD-specific glutamate dehydrogenase from Agaricus bisporus (1029 aa), FASTA scores: opt: 194, E(): 0.00099, (22.7% identity in 647 aa overlap) (see Kersten et al., 1999); etc. Contains possible Helix-turn-helix motif at aa 1568 to 1589 (score 1098, +2.93 SD). O53203 No intermediary metabolism and respiration O53203 O53203 GO:0004352,GO:0055114 1.4.1.2 NC_000962.3 Mycobrowser_v5 CDS 2786914 2789283 . - 0 Rv2482c plsB2 Involved in phospholipid biosynthesis (at the first step). May also function in the regulation of membrane biogenesis [catalytic activity: acyl-CoA + SN-glycerol 3-phosphate = CoA + 1-acyl-SN-glycerol 3-phosphate]. Probable glycerol-3-phosphate acyltransferase PlsB2 (GPAT) Rv2482c, (MT2555, MTV008.38c), len: 789 aa. Probable plsB2, glycerol-3-phosphate acyltransferase, highly similar to Q9X7B0|PLSB_MYCLE probable glycerol-3-phosphate acyltransferase from Mycobacterium leprae (775 aa), FASTA scores: opt: 4210, E(): 0, (80.7% identity in 783 aa overlap). Also similar to others e.g. P00482|PLSB_ECOLI from Escherichia coli (806 aa), FASTA scores: opt: 521, E(): 3e-24, (24.35 identity in 612 aa overlap); Q9CLN7|PLSB_PASMU from Pasteurella multocida (809 aa), FASTA scores: opt: 529, E(): 9.7e-25, (27.05% identity in 540 aa overlap); Q9KVP8|PLSB_VIBCH from Vibrio cholerae (811 aa), FASTA scores: opt: 510, E(): 1.4e-23, (26.0% identity in 639 aa overlap); etc. Also highly similar to Q10775|PLSB1|Rv1551|MTCY48.14c from M. tuberculosis (621 aa), FASTA scores: opt: 1013, E(): 1.5e-54, (34.65% identity in 586 aa overlap). Belongs to the GPAT/DAPAT family. P9WI61 No lipid metabolism O53207 P9WI61 GO:0008654,GO:0005886,GO:0004366 2.3.1.15 P9WI61 NC_000962.3 Mycobrowser_v5 CDS 2792723 2793988 . - 0 Rv2485c lipQ Function unknown; lipolytic enzyme involved in cellular metabolism. Probable carboxylesterase LipQ Rv2485c, (MTV008.41c), len: 421 aa. Probable lipQ, carboxylesterase protein (lipase). Similar (greater at the C-terminal end) to AAK46626|MT2342 putative carboxylesterase from Mycobacterium tuberculosis strain CDC1551 (431 aa), FASTA scores: opt: 1134, E(): 4.3e-60, (46.25% identity in 428 aa overlap); and Q50681|Rv2284|MTCY339.26c hypothetical protein from M. tuberculosis strain H37Rv (431 aa), FASTA scores: opt: 1134, E(): 4.3e-60, (46.25% identity in 428 aa overlap). Also similar in part to other putative lipases/esterases e.g. AAK44451|MT0230 from Mycobacterium tuberculosis strain CDC1551 (403 aa), FASTA scores: opt: 763, E(): 4.6e-38, (37.95% identity in 390 aa overlap); Q9RY19|DR0133 from Deinococcus radiodurans (296 aa), FASTA scores: opt: 392, E(): 4e-16, (33.7% identity in 276 aa overlap); Q9Z545|SC9B2.14 from Streptomyces coelicolor (502 aa) FASTA scores: opt: 279, E(): 3.2e-09, (31.15% identity in 292 aa overlap); etc. I6Y9F7 No intermediary metabolism and respiration O53210 I6Y9F7 GO:0016787 3.1.-.- I6Y9F7 NC_000962.3 Mycobrowser_v5 CDS 2801254 2806236 . - 0 Rv2490c PE_PGRS43 Function unknown PE-PGRS family protein PE_PGRS43 Rv2490c, (MTV008.46c), len: 1660 aa. PE_PGRS43, Member of the Mycobacterium tuberculosis PE family, PGRS-subfamily of Gly-rich proteins (see Brennan and Delogu, 2002), similar to many e.g. AAK47971|MT3612.1 PE_PGRS family protein from Mycobacterium tuberculosis strain CDC1551 (1715 aa), FASTA scores: opt: 5161, E(): 1.5e-187, (51.7% identity in 1752 aa overlap); etc. Q79FD4 No PE/PPE Q79FD4 Q79FD4 Q79FD4 NC_000962.3 Mycobrowser_v5 CDS 2808758 2809939 . - 0 Rv2495c bkdC Involved in energy metabolism. The branched-chain alpha-keto acid dehydrogenase complex catalyzes the overall conversion of branched chain alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Probable branched-chain keto acid dehydrogenase E2 component BkdC Rv2495c, (MTCY07A7.01c-MTV008.51c), len: 393 aa. Probable bkdC, branched-chain keto acid dehydrogenase, E2 component, similar to others e.g. Q9XA49|SCGD3.30c from Streptomyces coelicolor (491 aa) FASTA scores: opt: 615, E(): 1.2e-28, (36.45% identity in 491 aa overlap; several gaps); P19262|ODO2_YEAST|KGD2|YDR148C|YD8358.05c from Saccharomyces cerevisiae (Baker's yeast) (463 aa) FASTA scores: opt: 533, E(): 7.1e-24, (28.55% identity in 396 aa overlap); Q9HN75|DSA|VNG2219G from Halobacterium sp. strain NRC-1 (478 aa), FASTA scores: opt: 521, E(): E(): 3.7e-23, (30.25% identity in 486 aa overlap; in part); etc. Belongs to the 2-oxoacid dehydrogenase family. Alternative nucleotide at position 2809621 (T->C; T107A) has been observed. LpdC|Rv0462 co-immunoprecipitates with DlaT|Rv2215 (in lpdC|Rv0462 mutant) and with BkdC|Rv2495c (in dlaT|Rv2215 mutant) (See Venugopal et al., 2011). Previously known as pdhC. O06159 No intermediary metabolism and respiration 3L60 O06159 O06159 GO:0031405,GO:0008152,GO:0005515,GO:0004742 2.3.1.12 O06159 NC_000962.3 Mycobrowser_v5 CDS 2814916 2816880 . - 0 Rv2501c accA1 This protein carries two functions: biotin carboxyl carrier protein and biotin carboxyltransferase. Involved in the first step of long-chain fatty acid synthesis [catalytic activity: ATP + biotin-carboxyl-carrier protein + CO(2) = ADP + phosphate + carboxybiotin-carboxyl-carrier protein]. Probable acetyl-/propionyl-coenzyme A carboxylase alpha chain (alpha subunit) AccA1: biotin carboxylase + biotin carboxyl carrier protein (BCCP) Rv2501c, (MTCY07A7.07c, P46401), len: 654 aa. Probable accA1 (alternate gene name: bccA), acetyl-/propionyl-coenzyme A carboxylase (alpha subunit) [includes: biotin carboxylase ; biotin carboxyl carrier protein (BCCP)], similar to others eg Q9L076|FABG from Streptomyces coelicolor (646 aa), FASTA scores: opt: 2071, E(): 1e-113, (57.8% identity in 659 aa overlap); AAK24139|Q9A6C6|CC2168 from Caulobacter crescentus (654 aa), FASTA scores: opt: 1754, E(): 3.7e-95, (47.2% identity in 661 aa overlap); etc. Contains PS00188 Biotin-requiring enzymes attachment site, PS00866 Carbamoyl-phosphate synthase subdomain signature 1, and PS00867 Carbamoyl-phosphate synthase subdomain signature 2. P9WPQ3 No lipid metabolism P0A508 P9WPQ3 GO:0009374,GO:0004075,GO:0006633,GO:0005524 6.3.4.14 P9WPQ3 NC_000962.3 Mycobrowser_v5 CDS 2821712 2822359 . + 0 Rv2506 Rv2506 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably TetR-family) Rv2506, (MTCY07A7.12), len: 215 aa. Probable transcriptional regulator, TetR family, similar to many others e.g. Q9L078|SCC105.06c putative TetR-family regulatory protein from Streptomyces coelicolor (208 aa), FASTA scores: opt: 333, E(): 1.5e-14, (48.75% identity in 197 aa overlap); Q9X7X6|SC6A5.30c putative regulatory protein from Streptomyces coelicolor (404 aa), FASTA scores: opt: 267, E(): 4.8e-10, (30.45% identity in 207 aa overlap) (similarity only with C-terminus for this one); Q9FBI8|SCP8.33c putative TetR-family transcriptional regulator from Streptomyces coelicolor (213 aa), FASTA scores: opt: 239, E(): 1.8e-08, (29.9% identity in 184 aa overlap); etc. Also similar to transcriptional regulatory proteins from Mycobacterium tuberculosis e.g. O05858|Rv3208|MTCY07D11.18c (228 aa), FASTA scores: opt: 218, E(): 4.4e-07, (30.35% identity in 191 aa overlap); C-terminus of P95251|Rv1963c|MTV051.01c|MTCY09F9.01 (406 aa), FASTA scores: opt: 238, E(): 3.6e-08, (28.25% identity in 177 aa overlap); P96839|Rv3557c|MTCY06G11.04c (200 aa), FASTA scores: opt: 215, E(): 6.2e-07, (38.25% identity in 148 aa overlap); etc. Equivalent to AAK46885 from Mycobacterium tuberculosis strain CDC1551 (231 aa) but shorter 16 aa. Contains probable helix-turn-helix motif at aa 46-67, (Score 1660, +4.84 SD). Belongs to the TetR/AcrR family of transcriptional regulators. O06169 No regulatory proteins O06169 O06169 GO:0006350,GO:0003700,GO:0006355 O06169 NC_000962.3 Mycobrowser_v5 CDS 2925734 2926138 . + 0 Rv2596 vapC40 Unknown Possible toxin VapC40. Contains PIN domain. Rv2596, (MTCY227.05c), len: 134 aa. Possible vapC40, toxin, part of toxin-antitoxin (TA) operon with Rv2595, contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. O07780|Rv0598c|MTCY19H5.24 hypothetical 14.8 KDA protein from (137 aa), FASTA scores: opt: 254, E(): 8.8e-11, (41.55% identity in 130 aa overlap). P9WF61 No virulence, detoxification, adaptation Q50625 P9WF61 P9WF61 NC_000962.3 Mycobrowser_v5 CDS 2824678 2825484 . + 0 Rv2509 Rv2509 Function unknown; possibly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv2509, (MTCY07A7.15), len: 268 aa. Probable ala-rich oxidoreductase, short-chain dehydrogenase/reductase, equivalent to O07709|MLCL383.36c|ML0429 dehydrogenase (putative oxidoreductase) from Mycobacterium leprae (268 aa), FASTA scores: opt: 1509, E(): 2.6e-84, (88.75% identity in 267 aa overlap). Also highly similar to others e.g. O86553|SC1F2.16c putative dehydrogenase from Streptomyces coelicolor (276 aa), FASTA scores: opt: 492, E(): 9.5e-23, (38.15% identity in 262 aa overlap); Q9I5R3|PA0658 probable short-chain dehydrogenase from Pseudomonas aeruginosa (266 aa), FASTA scores: opt: 472, E(): 1.5e-21, (37.8% identity in 246 aa overlap); AAK22120|CC0133 oxidoreductase (short-chain dehydrogenase/reductase family) from Caulobacter crescentus (266 aa), FASTA scores: opt: 428, E(): 6.9e-19, (35.8% identity in 243 aa overlap); etc. Also highly similar or similar to oxidoreductases from Mycobacterium tuberculosis e.g. Q10782|Rv1544|MTCY48.21 putative ketoacyl reductase (267 aa), FASTA scores: opt: 656, E(): 1.1e-32, (43.05% identity in 267 aa overlap). Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. I6Y9I3 No intermediary metabolism and respiration O06172 I6Y9I3 GO:0055114,GO:0016491 1.-.-.- I6Y9I3 NC_000962.3 Mycobrowser_v5 CDS 2834109 2835335 . - 0 Rv2518c ldtB Involved in peptidoglycan synthesis. Catalyzes the formation of 3->3 crosslinks between peptidoglycan subunits. Probable L,D-transpeptidase LdtB Rv2518c, (MTV009.03c), len: 408 aa. Probable ldtB, L,D-transpeptidase, highly similar to O07707|MLCL383.3 hypothetical 43.6 KDA protein from Mycobacterium leprae (407 aa), FASTA scores: opt: 2300, E(): 1.2e-130, (82.5% identity in 406 aa overlap); Q9CCU5|LPPS|ML0426 putative secreted protein from Mycobacterium leprae (404 aa), FASTA scores: opt: 2279, E(): 2.3e-129, (82.4% identity in 403 aa overlap); and Q9CB49|ML2446 possible lipoprotein from Mycobacterium leprae (441 aa), FASTA scores: opt: 736, E(): 8.4e-37, (35.6% identity in 399 aa overlap). Also similar to other proteins from several organisms e.g. Q9X811|SC6G10.26c putative secreted protein from Streptomyces coelicolor (424 aa), FASTA scores: opt: 867, E(): 1.1e-44, (32.25% identity in 403 aa overlap); Q9L1E8|SC3D11.14 putative lipoprotein from Streptomyces coelicolor (416 aa), FASTA scores: opt: 737, E(): 7e-37, (32.95% identity in 413 aa overlap); Q9KYV1|SCE22.11 putative lipoprotein from Streptomyces coelicolor (407 aa), FASTA scores: opt: 721, E(): 6.2e-36, (33.5% identity in 400 aa overlap). And similar to several hypothetical mycobacterial proteins e.g. Q11149|Y483_MYCTU|Rv0483|MT0501|MTCY20G9.09 (451 aa), FASTA scores: opt: 763, E(): 2.1e-38, (34.85% identity in 402 aa overlap). Has very long signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Note that previously known as lppS I6Y9J2 No cell wall and cell processes O53223 I6Y9J2 I6Y9J2 NC_000962.3 Mycobrowser_v5 CDS 2838129 2839541 . - 0 Rv2522c Rv2522c Function unknown Conserved hypothetical protein Rv2522c, (MTV009.07c), len: 470 aa. Conserved hypothetical protein, equivalent, but longer 20 aa, to Q9X7E4|ML1193|MLCB458.08 from hypothetical 46.6 KDA protein Mycobacterium leprae (442 aa), FASTA scores: opt: 2521, E(): 4.1e-142, (86.35% identity in 440 aa overlap). Also similar to various proteins e.g. Q9K425|SCG22.20 putative peptidase from Streptomyces coelicolor (451 aa), FASTA scores: opt: 1097, E(): 1.1e-57, (42.5% identity in 451 aa overlap); Q9FCK3|2SC3B6.09 putative peptidase from Streptomyces coelicolor (470 aa), FASTA scores: opt: 669, E(): 2.8e-32, (34.2% identity in 462 aa overlap); Q98AF9|MLL6018 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (486 aa), FASTA scores: opt: 622, E(): 1.7e-29, (33.95% identity in 442 aa overlap); Q9RSU7|DR2025 ARGE/DAPE/ACY1 family protein from Deinococcus radiodurans (459 aa), FASTA scores: opt: 616, E(): 3.7e-29, (34.15% identity in 442 aa overlap); etc (include some similarity to hypothetical proteins from C. elegans and yeast). Alternative start possible at 6687 but then no RBS obvious. I6X4J0 No conserved hypotheticals O53227 I6X4J0 GO:0008152,GO:0005515,GO:0016787 I6X4J0 NC_000962.3 Mycobrowser_v5 CDS 2839538 2839930 . - 0 Rv2523c acpS Biosynthesis of fatty acids and lipids. Transfers the 4'-phosphopantetheine moiety from coenzyme A to a SER of acyl-carrier protein. Catalyzes the formation of holo-ACP, which mediates the transfer of acyl fatty-acid intermediates during the biosynthesis of fatty acids and lipids [catalytic activity: CoA + APO-[acyl-carrier protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] ]. holo-[acyl-carrier protein] synthase AcpS (holo-ACP synthase) (CoA:APO-[ACP]pantetheinephosphotransferase) (CoA:APO-[acyl-carrier protein]pantetheinephosphotransferase) Rv2523c, (MT2599, MTV009.08c), len: 130 aa. AcpS, holo-[Acyl Carrier Protein] synthase (see citation below), equivalent to Q9X7E3|ACPS_MYCLE|ML1192|MLCB458.07 holo-[acyl-carrier protein] synthase from Mycobacterium leprae (130 aa), FASTA scores: opt: 732, E(): 5.5e-42, (87.5% identity in 128 aa overlap). Also similar to others e.g. O86785|ACPS_STRCO|SC6G4.22c from Streptomyces coelicolor (123 aa), FASTA scores: opt: 204, E(): 6.6e-07, (36.7% identity in 139 aa overlap); Q9KPB6|VC2457 from Vibrio cholerae (126 aa), FASTA scores: opt: 163, E(): 0.00036, (32.55% identity in 129 aa overlap); P24224|ACPS_ECOLI|DPJ|B2563 from Escherichia coli strain K12 (125 aa), FASTA scores: opt: 151, E(): 0.0022, (30.55% identity in 131 aa overlap); etc. Belongs to the ACPS family. Acts on fas-I enzymes in C. glutamicum (See Chalut et al., 2006). P9WQD3 No lipid metabolism 3H7Q,3HQJ,3NE1,3NE3 P0A4W8 P9WQD3 GO:0006633,GO:0008897,GO:0009059,GO:0000287,GO:0005737 2.7.8.7 P9WQD3 NC_000962.3 Mycobrowser_v5 CDS 2851751 2852671 . - 0 Rv2528c mrr Involved in the acceptance of foreign DNA which is modified. Restricts both adenine- and cytosine-methylated DNA. Probable restriction system protein Mrr Rv2528c, (MTCY159.28), len: 306 aa. Probable mrr, restriction system protein, similar to other mrr proteins e.g. Q9RWS8|DR0587|MRR from Deinococcus radiodurans (306 aa), FASTA scores: opt: 776, E(): 4.2e-40, (40.45% identity in 309 aa overlap); P24202|MRR_ECOLI|B4351 from Escherichia coli strain K12 (304 aa), FASTA scores: opt: 647, E(): 2.9e-32, (35.25% identity in 309 aa overlap); Q9RX07|DR0508 from Deinococcus radiodurans (336 aa), FASTA scores: opt: 456, E(): 1.3e-20, (37.3% identity in 319 aa overlap); etc. I6Y9K2 No information pathways P95025 I6Y9K2 GO:0009307,GO:0004519,GO:0003677 I6Y9K2 NC_000962.3 Mycobrowser_v5 CDS 2858727 2859290 . - 0 Rv2534c efp Involved in peptide bond synthesis. Stimulate efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase. Probable elongation factor P Efp Rv2534c, (MTCY159.22), len: 187 aa. Probable efp, elongation factor P, equivalent to Q9CCS0|EFP|ML0522 elongation factor P from Mycobacterium leprae (187 aa), FASTA scores: opt: 1158, E(): 2.1e-67, (94.1% identity in 186 aa overlap). Also highly similar to many e.g. Q45288|EFP_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (187 aa), FASTA scores: opt: 843, E(): 3.4e-47, (69.5% identity in 187 aa overlap); Q9KXQ9|EFP from Streptomyces coelicolor (188 aa), FASTA scores: opt: 833, E(): 1.5e-46, (67.0% identity in 188 aa overlap); P49778|EFP_BACSU from Bacillus subtilis (185 aa), FASTA scores: opt: 607, E(): 4.6e-32, (47.8% identity in 182 aa overlap); P33398|EFP_ECOLI|B4147 from Escherichia coli strain K12 (187 aa), FASTA scores: opt: 503, E(): 1.8e-27, (42.3% identity in 182 aa overlap); etc. Belongs to the elongation factor P family. P9WNM3 No information pathways P64034 P9WNM3 GO:0003746,GO:0006414,GO:0005737 P9WNM3 NC_000962.3 Mycobrowser_v5 CDS 2859300 2860418 . - 0 Rv2535c pepQ Function unknown; hydrolyses peptides. Probable cytoplasmic peptidase PepQ Rv2535c, (MTCY159.21), len: 372 aa. Probable pepQ, cytoplasmic peptidase, equivalent to Q9CCS1|PEPQ|ML0521 putative cytoplasmic peptidase from Mycobacterium leprae (376 aa), FASTA scores: opt: 1954, E(): 1.1e-105, (82.7% identity in 376 aa overlap). Also similar to other peptidases e.g. P54518|YQHT_BACSU putative peptidase (belongs to peptidase family M24B) from Bacillus subtilis (353 aa), FASTA scores: opt: 808, E(): 1.6e-39, (39.65% identity in 368 aa overlap); Q9KXQ8|SC9C5.16c putative peptidase from Streptomyces coelicolor (368 aa), FASTA scores: opt: 803, E(): 3.2e-39, (43.15% identity in 380 aa overlap); Q9K950|BH2800 XAA-pro dipeptidase from Bacillus halodurans (355 aa), FASTA scores: opt: 801, E(): 4.1e-39, (39.45% identity in 365 aa overlap); etc. Note that second part of protein is similar to second part of MTCY49.29c|Rv2089c|MT2150|MTCY49.29c probable dipeptidase; belongs to peptidase family M24B from Mycobacterium tuberculosis (375 aa) (33.9% identity in 354 aa overlap) blast results: Score: 142 bits (359), E: 4e-33, Identities: 86/224 (38%), Positives: 119/224 (52%), Gaps: 4/224 (1%). Could be belong to peptidase family M24B. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). I6YDN6 No intermediary metabolism and respiration P95018 I6YDN6 GO:0009987,GO:0016805,GO:0046872,GO:0008235,GO:0006508,GO:0004177 3.4.13.9 I6YDN6 NC_000962.3 Mycobrowser_v5 CDS 2860452 2861144 . + 0 Rv2536 Rv2536 Unknown Probable conserved transmembrane protein Rv2536, (MTCY159.20c), len: 230 aa. Probable conserved transmembrane protein, equivalent to Q9CCS2|ML0520 putative membrane protein from Mycobacterium leprae (202 aa), FASTA scores: opt: 812, E(): 2e-41, (63.2% identity in 201 aa overlap). Also similar in part to Q9HMD5|VNG2594c from Halobacterium sp. strain NRC-1 (117 aa), FASTA scores: opt: 33.6, E(): 1.8, (33.6% identity in 116 aa overlap); and perhaps AAK65752|SMA1996 putative ABC transporter permease protein from Rhizobium meliloti (Sinorhizobium meliloti) plasmid pSymA (323 aa), FASTA scores: opt: 117, E(): 6.1, (30.6% identity in 121 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P95017 No cell wall and cell processes P95017 P95017 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2861148 2861591 . - 0 Rv2537c aroD Involved at the third step in the biosynthesis of chorismate within the biosynthesis of aromatic amino acids (the shikimate pathway). Catalyze a trans-dehydration via an enolate intermediate [catalytic activity: 3-dehydroquinate = 3-dehydroshikimate + H(2)O]. 3-dehydroquinate dehydratase AroD (AROQ) (3-dehydroquinase) (type II dhqase) Rv2537c, (MTCY159.19), len: 147 aa. AroD (alternate gene name: aroQ), 3-dehydroquinate dehydratase (see citation below), equivalent to Q9CCS3|AROD|ML0519 3-dehydroquinate dehydratase from Mycobacterium leprae (145 aa), FASTA scores: opt: 803, E(): 3.4e-46, (85.9% identity in 142 aa overlap). Also highly similar to many e.g. P96750|AROQ_CORPS from Corynebacterium pseudotuberculosis (146 aa), FASTA scores: opt: 559, E(): 4.1e-30, (61.05% identity in 136 aa overlap); Q9K949|BH2801 from Bacillus halodurans (145 aa), FASTA scores: opt: 453, E(): 4e-23, (52.15% identity in 138 aa overlap); P54517|AROQ_BACSU|YQHS from Bacillus subtilis (148 aa), FASTA scores: opt: 419, E(): 7.1e-21, (45.3% identity in 139 aa overlap); etc. Contains PS01029 Dehydroquinase class II signature. Belongs to the type-II 3-dehydroquinase family. P9WPX7 No intermediary metabolism and respiration 1H05,1H0R,1H0S,2DHQ,2Y71,2Y76,2Y77,2XB8,3N59,3N76,3N7A,3N86,3N87,3N8K,3N8N P0A4Z6 P9WPX7 GO:0009073,GO:0003855 4.2.1.10 P9WPX7 NC_000962.3 Mycobrowser_v5 CDS 2861588 2862676 . - 0 Rv2538c aroB Involved at the second step in the biosynthesis of chorismate within the biosynthesis of aromatic amino acids (the shikimate pathway) [catalytic activity: 7-phospho-3-deoxy-arabino-heptulosonate = 3-dehydroquinate + orthophosphate]. 3-dehydroquinate synthase AroB Rv2538c, (MTCY159.18), len: 362 aa. AroB, 3-dehydroquinate synthase (see citations below), equivalent to Q9CCS4|AROB_MYCLE|ML0518 3-dehydroquinate synthase from Mycobacterium leprae (361 aa), FASTA scores: opt: 2059, E(): 3.3e-117, (87.25% identity in 361 aa overlap). Also highly similar to many e.g. Q9KXQ6|AROB from Streptomyces coelicolor (363 aa), FASTA scores: opt: 1363, E(): 4e-75, (60.05% identity in 358 aa overlap); Q9X5D2|AROB_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (366 aa), FASTA scores: opt: 1154, E(): 1.7e-62, (50.95% identity in 359 aa overlap); P07639|AROB_ECOLI|B3389 from Escherichia coli strain K12 (362 aa), FASTA scores: opt: 771, E(): 2.4e-39, (40.6% identity in 345 aa overlap); etc. Belongs to the dehydroquinate synthase family. P9WPX9 No intermediary metabolism and respiration P0A4Z4 P9WPX9 GO:0009073,GO:0005737,GO:0003856 4.2.3.4 P9WPX9 NC_000962.3 Mycobrowser_v5 CDS 2868860 2869237 . + 0 Rv2548 vapC19 Unknown Possible toxin VapC19 Rv2548, (MTCY159.08c), len: 125 aa. Possible vapC19, toxin, part of toxin-antitoxin (TA) operon with Rv2547, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similarity to others in Mycobacterium tuberculosis e.g. P71665|Rv1397c|MTCY21B4.14c hypothetical 15.0 KDA protein (133 aa), FASTA scores: opt: 265, E(): 7.1e-12, (42.3% identity in 123 aa overlap); and to Q97WY5|SSO1975 hypothetical protein from Sulfolobus solfataricus (125 aa), FASTA scores: opt: 131, E(): 0.018, (30.0% identity in 110 aa overlap); O52285|YLE hypothetical 14.9 KDA protein from Agrobacterium radiobacter (133 aa), FASTA scores: opt: 128, E(): 0.03, (32.8% identity in 125 aa overlap); etc. P9WF93 No virulence, detoxification, adaptation P95005 P9WF93 P9WF93 NC_000962.3 Mycobrowser_v5 CDS 2871206 2872015 . - 0 Rv2552c aroE Possibly involved at the fourth step in the biosynthesis of chorismate within the biosynthesis of aromatic amino acids (the shikimate pathway) [catalytic activity: shikimate + NADP(+) = 5-dehydroshikimate + NADPH]. Probable shikimate 5-dehydrogenase AroE (5-dehydroshikimate reductase) Rv2552c, (MTCY159.04), len: 269 aa. Probable aroE, shikimate 5-dehydrogenase, equivalent to Q9CCS7|AROE|ML0515 putative shikimate 5-dehydrogenase from Mycobacterium leprae (278 aa), FASTA scores: opt: 1452, E(): 1.8e-77, (81.5% identity in 270 aa overlap). Also highly similar, but longer 101 aa, to Q9KH59|AROE putative shikimate dehydrogenase (fragment) from Mycobacterium marinum (148 aa), FASTA scores: opt: 729, E(): 1.3e-35, (76.35% identity in 148 overlap); Q9F7W3|AROE from Mycobacterium ulcerans (148 aa), FASTA scores: opt: 718, E(): 5.9e-35, (75.7% identity in 148 aa overlap). And also similar to to others e.g. Q9KXQ2|AROE from Streptomyces coelicolor (255 aa), FASTA scores: opt: 572, E(): 2.8e-26, (43.4% identity in 251 aa overlap); Q98DY3|MLR4492 from Rhizobium loti (Mesorhizobium loti) (280 aa), FASTA scores: opt: 385, E(): 2.2e-15, (34.85% identity in 284 aa overlap); P74591|AROE_SYNY3|SLR1559 from Synechocystis sp. strain PCC 6803 (290 aa), FASTA scores: opt: 347, E(): 3.7e-13, (30.9% identity in 275 aa overlap); P15770|AROE_ECOLI|B3281 from Escherichia coli strain K12 (272 aa), FASTA scores: opt: 230, E(): 7.7e-08, (29.5% identity in 251 aa overlap); etc. Belongs to the shikimate dehydrogenase family. I6Y120 No intermediary metabolism and respiration P95001 I6Y120 GO:0005737,GO:0055114,GO:0004764 1.1.1.25 I6Y120 NC_000962.3 Mycobrowser_v5 CDS 2873771 2876485 . - 0 Rv2555c alaS Involved in translation mechanism [catalytic activity: ATP + L-alanine + tRNA(ala) = AMP + pyrophosphate + L-alanyl-tRNA (ala)]. Probable alanyl-tRNA synthetase AlaS (alanine--tRNA ligase) (alanine translase) (ALARS) Rv2555c, (MTCY318.01c-MTCY159.01), len: 904 aa. Probable alaS, alanyl-tRNA synthetase, equivalent to Q9CCT0|alas|ML0512 alanyl-tRNA synthetase from Mycobacterium leprae (908 aa), FASTA scores: opt: 5013, E(): 0, (84.65% identity in 907 aa overlap). Also highly similar to many e.g. Q9KXP9|alas from Streptomyces coelicolor (890 aa), FASTA scores: opt: 2159, E(): 3.8e-118, (53.45% identity in 907 aa overlap); Q9FFC7 Arabidopsis thaliana (Mouse-ear cress) (954 aa), FASTA scores: opt: 1963, E(): 1.1e-106, (41.1% identity in 925 aa overlap); Q9RS27|DR2300 from Deinococcus radiodurans (890 aa), FASTA scores: opt: 1352, E(): 4.1e-71, (38.05% identity in 915 aa overlap); etc. Belongs to class-II aminoacyl-tRNA synthetase family. P9WFW7 No information pathways O07438 P9WFW7 GO:0004813,GO:0006419,GO:0005737,GO:0046872,GO:0000049,GO:0005524 6.1.1.7 P9WFW7 NC_000962.3 Mycobrowser_v5 CDS 2878571 2879929 . - 0 Rv2559c Rv2559c Function unknown Conserved hypothetical alanine leucine valine rich protein Rv2559c, (MTCY9C4.09), len: 452 aa. Conserved hypothetical ala-, leu-, val-rich protein, equivalent to Q9CCT1|ML0510 hypothetical protein from Mycobacterium leprae (473 aa), FASTA scores: opt: 2411, E(): 3.9e-121, (83.4% identity in 452 aa overlap); O69490|O69490 hypothetical 47.1 KDA protein from Mycobacterium leprae (447 aa), FASTA scores: opt: 2406, E(): 6.9e-121, (83.95% identity in 448 aa overlap). Also highly similar to Q9KXP4|SC9C5.30c conserved ATP/GTP binding protein from Streptomyces coelicolor (451 aa), FASTA scores: opt: 1742, E(): 1.5e-85, (64.4% identity in 430 aa overlap); Q9RT67|DR1898 conserved hypothetical protein from Deinococcus radiodurans (434 aa), FASTA scores: opt: 1147, E(): 6.6e-54, (46.0% identity in 415 aa overlap); P45262|YCAJ_HAEIN|HI1590 hypothetical protein from Haemophilus influenzae (446 aa), FASTA scores: opt: 1140, E(): 1.6e-53, (42.5% identity in 428 aa overlap); etc. Also similar to Q50629|MTCY227.09|RUVB|Rv2592c|MT2669|MTCY227.09 holliday junction DNA helicase from Mycobacterium tuberculosis (344 aa), (30.1% identity in 296 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). P9WQN1 No conserved hypotheticals Q50739 P9WQN1 GO:0017111,GO:0005524 P9WQN1 NC_000962.3 Mycobrowser_v5 CDS 2883342 2884334 . + 0 Rv2564 glnQ Thought to be involved in active transport of glutamine across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable glutamine-transport ATP-binding protein ABC transporter GlnQ Rv2564, (MTCY9C4.04c), len: 330 aa. Probable glnQ, glutamine-transport ATP-binding protein ABC transporter (see citation below), highly similar to many e.g. Q9L0J9|SCD40A.12c putative ABC-transporter ATP-binding protein from Streptomyces coelicolor (246 aa), FASTA scores: opt: 598, E(): 2.5e-26, (46.35% identity in 218 aa overlap); O54136|SC2E9.11 from Streptomyces coelicolor (230 aa), FASTA scores: opt: 592, E(): 5.1e-26, (46.55% identity in 219 aa overlap); O29244|AF1018 from Archaeoglobus fulgidus (228 aa), FASTA scores: opt: 580, E(): 2.4e-25, (42.4% identity in 210 aa overlap); P75831|YBJZ_ECOLI|B0879 from Escherichia coli strain K12 (648 aa), FASTA scores: opt: 555, E(): 1.3e-23, (39.65% identity in 232 aa overlap); etc. Also highly similar to O53618|Rv0073|MTV030.17 ABC-transporter ATP-binding subunit from Mycobacterium tuberculosis (330 aa), FASTA scores: opt: 1782, E(): 4.7e-92, (83.65% identity in 330 aa overlap); etc. Shows some similarity to Q11040|YC81_MYCTU|MTCY50.01|Rv1281c|MT1318 hypothetical ABC transporter ATP-binding protein from Mycobacterium tuberculosis (612 aa) (32.9 % identity in 234 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00211 ABC transporters family signature, and PS00889 Cyclic nucleotide-binding domain signature 2. Belongs to the ATP-binding transport protein family (ABC transporters). P9WQI5 No cell wall and cell processes P63401 P9WQI5 GO:0016887,GO:0005952,GO:0008603,GO:0001932,GO:0006810,GO:0005524 P9WQI5 NC_000962.3 Mycobrowser_v5 CDS 2884611 2886362 . + 0 Rv2565 Rv2565 Function unknown Conserved protein Rv2565, (MTCY9C4.03c), len: 583 aa. Conserved protein, similar in part to Q9A6C3|CC2171 hypothetical protein from Caulobacter crescentus (610 aa), FASTA scores: opt: 765, E(): 2.8e-37, (32.15% identity in 575 aa overlap). C-terminus also highly similar to various bacterial proteins e.g. O34731|YLBK_BACSU hypothetical 28.3 KDA protein from Bacillus subtilis (260 aa), FASTA scores: opt: 386, E(): 2.2e-15, (33.05% identity in 245 aa overlap); CAC45997|SMC01003 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (321 aa), FASTA scores: opt: 352, E(): 2.5e-13, (29.65% identity in 280 aa overlap); Q9K9Q8|BH2587 hypothetical protein from Bacillus halodurans (275 aa), FASTA scores: opt: 334, E(): 2.5e-12, (33.7% identity in 175 aa overlap); etc. And shows similarity to C-terminal half of some eukaryotic proteins e.g. Q9R114|NTE neuropathy target esterase homolog from Mus musculus (Mouse) (1327 aa), FASTA scores: opt: 411, E(): 2.7e-16, (24.45% identity in 626 aa overlap); O60859 neuropathy target esterase from Homo sapiens (Human) (1327 aa), FASTA scores: opt: 410, E(): 3.1e-16, (24.1% identity in 627 aa overlap); Q9U969|SWS|CG2212 swiss cheese protein from Drosophila melanogaster (Fruit fly) (1425 aa), FASTA scores: opt: 401, E(): 1.1e-15, (27.75% identity in 544 aa overlap); etc. Also shows strong similarity to C-terminal half of O05884|Z95121|Rv3239c|MTY20B11.14c hypothetical 110.2 KDA protein from Mycobacterium tuberculosis (1048 aa), FASTA scores: opt: 648, E(): 3e-30, (36.55% identity in 572 aa overlap); and O69695|Rv3728|MTV025.076 putative two-domain membrane protein from Mycobacterium tuberculosis (1065 aa), FASTA scores: opt: 643, E(): 6e-30, (34.3% identity in 595 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). P9WIY7 No conserved hypotheticals P0A642 P9WIY7 GO:0004622,GO:0046470,GO:0005789 P9WIY7 NC_000962.3 Mycobrowser_v5 CDS 2886373 2889795 . + 0 Rv2566 Rv2566 Function unknown Long conserved protein Rv2566, (MTCY9C4.02c), len: 1140 aa. Long conserved protein, equivalent to O53120|ML2678 or MLCB1913.12 hypothetical protein from Mycobacterium leprae (1000 aa), FASTA scores: opt: 760, E(): 7.1e-38, (50.2% identity in 1128 aa overlap); and middle part equivalent to Q9ZB40 72.2 KDA protein (fragment) from Mycobacterium leprae (644 aa), FASTA scores: opt: 1017, E(): 1.5e-65, (45.65% identity in 655 aa overlap). Also highly similar to Q98HG6|MLL2877 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (1119 aa), FASTA scores: opt: 1413, E(): 3.7e-77, (52.4% identity in 1148 aa overlap); and N-terminus shows similarity with other proteins e.g. Q9HUN8|PA4926 hypothetical protein from Pseudomonas aeruginosa (311 aa), FASTA scores: opt: 278, E(): 3e-09, (29.95% identity in 284 aa overlap); and upstream ORF Q50652|YP69_MYCTU|Rv2569c|MT2645|MTCY227.32 conserved hypothetical protein from Mycobacterium tuberculosis (314 aa), FASTA scores: opt: 252, E(): 1.1e-07, (28.9% identity in 315 aa overlap). Equivalent to AAK46955 from Mycobacterium tuberculosis strain CDC1551 (1156 aa) but shorter 16 aa. Q50732 No conserved hypotheticals Q50732 Q50732 Q50732 NC_000962.3 Mycobrowser_v5 CDS 2889795 2892449 . + 0 Rv2567 Rv2567 Function unknown Conserved hypothetical alanine and leucine rich protein Rv2567, (MTCY227.34c, MTCY9C4.01c), len: 884 aa. Conserved hypothetical ala-, leu-rich protein, equivalent to O53121|ML2679|MLCB1913.13 hypothetical protein from Mycobacterium leprae (893 aa), FASTA scores: opt: 4326, E(): 0, (75.2% identity in 883 aa overlap); and similar to Q49755|YO11_MYCLE|ML0605|MLCL536.05c|U1937B|B1937_F1_4 hypothetical 61.8 KDA protein from Mycobacterium leprae (561 aa), FASTA scores: opt: 758, E(): 1.2e-38, (32.2% identity in 537 aa overlap). Also similar to others e.g. Q9HUN7|PA4927 hypothetical protein from Pseudomonas aeruginosa (830 aa), FASTA scores: opt: 1247, E(): 2.2e-68, (38.25% identity in 831 aa overlap); Q98HG7|MLL2876 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (803 aa), FASTA scores: opt: 937, E(): 1.9e-49, (32.15% identity in 828 aa overlap); CAC47419|SMC04057 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (802 aa), FASTA scores: opt: 900, E(): 3.4e-47, (30.85% identity in 852 aa overlap); etc. And similar to P71732|YO11_MYCTU|Rv2411c|MT2484|MTCY253.09 conserved hypothetical protein from Mycobacterium tuberculosis (551 aa), FASTA scores: opt: 781, E(): 4.6e-40, (33.75% identity in 495 aa overlap). P9WL97 No conserved hypotheticals Q50654 P9WL97 P9WL97 NC_000962.3 Mycobrowser_v5 CDS 2896013 2897803 . - 0 Rv2572c aspS Involved in translation mechanism [catalytic activity: ATP + L-aspartate + tRNA(asp) = AMP + pyrophosphate + L-aspartyl-tRNA(asp)]. Probable aspartyl-tRNA synthetase AspS (aspartate--tRNA ligase) (ASPRS) (aspartic acid translase) Rv2572c, (MTCY227.29), len: 596 aa. Probable aspS, aspartyl-tRNA synthetase, equivalent to P36429|SYD_MYCLE|ML0501|MLCB1259.19 aspartyl-tRNA synthetase from Mycobacterium leprae (589 aa), FASTA scores: opt: 3534, E(): 1.8e-215, (87.85% identity in 592 aa overlap). Also highly similar to many e.g. O67589|SYD_AQUAE|AQ_1677 from Aquifex aeolicus (603 aa), FASTA scores: opt: 1829, E(): 8.2e-108, (47.5% identity in 598 aa overlap); O32038|SYD_BACSU from Bacillus subtilis (592 aa), FASTA scores: opt: 1732, E(): 1.1e-101, (46.25% identity in 597 aa overlap); P21889|SYD_ECOLI|TLS|B1866 from Escherichia coli strain K12 (590 aa), FASTA scores: opt: 1588, E(): 1.3e-92, (47.35% identity in 581 aa overlap); etc. Contains PS00179 Aminoacyl-transfer RNA synthetases class-II signature 1. Belongs to class-II aminoacyl-tRNA synthetase family. P9WFW3 No information pathways Q50649 P9WFW3 GO:0004815,GO:0006422,GO:0005737,GO:0003676,GO:0005524 6.1.1.12 P9WFW3 NC_000962.3 Mycobrowser_v5 CDS 2899339 2900220 . + 0 Rv2575 Rv2575 Unknown Possible conserved membrane glycine rich protein Rv2575, (MTCY227.26c), len: 293 aa. Possible conserved membrane gly-rich protein, highly similar to hypothetical proteins e.g. Q9RR98|DR2596 conserved hypothetical protein from Deinococcus radiodurans (313 aa), FASTA scores: opt: 734, E(): 2.8e-38, (42.95% identity in 291 aa overlap); Q9HV81|PA4717 from Pseudomonas aeruginosa (297 aa), FASTA scores: opt: 641, E(): 1.5e-32, (43.35% identity in 300 aa overlap); Q98IA4|MLL2493 from Rhizobium loti (Mesorhizobium loti) (306 aa), FASTA scores: opt: 628, E(): 1e-31, (38.45% identity in 307 aa overlap); etc. Contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. P9WL85 No cell wall and cell processes P65019 P9WL85 GO:0016021 P9WL85 NC_000962.3 Mycobrowser_v5 CDS 2900918 2902507 . + 0 Rv2577 Rv2577 Unknown Conserved protein Rv2577, (MTCY227.24c), len: 529 aa. Conserved protein, showing similarity with various proteins from eukaryotes, in particular phosphatases, e.g. Q9SE01|pap purple acid phosphatase precursor from Glycine max (Soybean) (464 aa), FASTA scores: opt: 190, E(): 0.00026, (27.3% identity in 388 aa overlap); Q9SVP2|F18A5.90|AT4G13700 hypothetical 53.4 KDA protein from Arabidopsis thaliana (Mouse-ear cress) (474 aa), FASTA scores: opt: 280, E(): 6.6e-10, (27.2% identity in 331 aa overlap); Q9FK32 similarity to unknown protein from Arabidopsis thaliana (Mouse-ear cress) (529 aa), FASTA scores: opt: 249, E(): 6.2e-08, (25.3% identity in 435 aa overlap); Q12546|APHA acid phosphatase precursor from Aspergillus ficuum (614 aa), FASTA scores: opt: 207, E(): 2.9e-05, (22.95% identity in 458 aa overlap); etc. P9WL81 No conserved hypotheticals Q50644 P9WL81 GO:0046872,GO:0003993 NC_000962.3 Mycobrowser_v5 CDS 2902509 2903531 . - 0 Rv2578c Rv2578c Function unknown Conserved hypothetical protein Rv2578c, (MTCY227.23), len: 340 aa. Conserved hypothetical protein, highly similar to hypothetical proteins (conserved or not) e.g. Q9ZBJ3|SC9C7.17c from Streptomyces coelicolor (348 aa), FASTA scores: opt: 998, E(): 1.6e-55, (47.6% identity in 355 aa overlap); Q9I763|PA0069 from Pseudomonas aeruginosa (352 aa), FASTA scores: opt: 560, E(): 6e-28, (36.6% identity in 284 aa overlap); Q986C9|MLL7417 from Rhizobium loti (Mesorhizobium loti) (356 aa), FASTA scores: opt: 550, E(): 2.6e-27, (39.15% identity in 240 aa overlap); etc. P9WL79 No conserved hypotheticals P65023 P9WL79 GO:0003824,GO:0016021,GO:0046872,GO:0005886,GO:0051539 P9WL79 NC_000962.3 Mycobrowser_v5 CDS 2903639 2904541 . + 0 Rv2579 dhaA Generates a primary alcohol and halide from 1-haloalkane and H2O [catalytic activity: 1-haloalkane + H2O = a primary alcohol + halide]. Possible haloalkane dehalogenase DhaA (1-chlorohexane halidohydrolase) Rv2579, (MTCY227.22c), len: 300 aa. Possible dhaA, haloalkane dehalogenase, strictly equivalent to Q9XB14|ISO-RV2579 haloalkane dehalogenase (1-chlorohexane halidohydrolase) from Mycobacterium bovis (300 aa), FASTA scores: opt: 2075, E(): 7.1e-125, (99.35% identity in 300 aa overlap); note that only two residues, 120 and 293 are different. Also highly similar to others e.g. Q9ZER0|DHAAF haloalkane dehalogenase from Mycobacterium sp strain GP1 (307 aa), FASTA scores: opt: 842, E(): 2.3e-46, (44.95% identity in 298 aa overlap); Q53042|DHAA haloalkane dehalogenase from Rhodococcus rhodochrous, and Pseudomonas pavonaceae (293 aa), FASTA scores: opt: 837, E(): 4.5e-46, (44.6% identity in 298 aa overlap); etc. Note that this protein may also be a 1,3,4,6-tetrachloro-1,4-cyclohexadiene hydrolase, because also highly similar to P51698|LINB_PSEPA 1,3,4,6-tetrachloro-1,4-cyclohexadiene hydrolase from Pseudomonas paucimobilis (Sphingomonas paucimobilis) (see Nagata et al., 1993) (296 aa), FASTA scores: opt: 1494, E(): 6.8e-88, (69.5% identity in 295 aa overlap). Also shows some similarity with proteins from Mycobacterium tuberculosis e.g. Q50670|YM96_MYCTU|Rv2296|MT2353|MTCY339.14c putative haloalkane dehalogenase (300 aa), FASTA scores: opt: 302, E(): 5.3e-12, (30.85% identity in 295 aa overlap); and Q50600|YJ33_MYCTU|Rv1833c|MT1881|MTCY1A11.10 hypothetical 32.2 KDA protein (286 aa), FASTA scores: opt: 286, E(): 5.3e-11, (29.85% identity in 288 aa overlap). May belong to alpha/beta hydrolase fold family. Note that previously known as linB. P9WMR9 No intermediary metabolism and respiration 2O2H,2O2I,2QVB Q50642 P9WMR9 GO:0018786 3.8.1.5 P9WMR9 NC_000962.3 Mycobrowser_v5 CDS 2904821 2906092 . - 0 Rv2580c hisS Involved in translation mechamism [catalytic activity: ATP + L-histidine + tRNA(his) = AMP + pyrophosphate + L-histidyl-tRNA(his)]. Probable histidyl-tRNA synthetase HisS (histidine--tRNA ligase) (HISRS) (histidine--translase) Rv2580c, (MT2657, MTCY227.21), len: 423 aa. Probable hisS, histidyl-tRNA synthetase, equivalent to P46696|SYH_MYCLE|hiss|ML0494|MLCB1259.12|B1177_C3_248 histidyl-tRNA synthetase from Mycobacterium leprae (427 aa), FASTA scores: opt: 2380, E(): 2.1e-131, (85.85% identity in 417 aa overlap). Also highly similar to many e.g. Q9KXP2|hiss from Streptomyces coelicolor (425 aa), FASTA scores: opt: 1542, E(): 1.4e-82, (56.0% identity in 418 aa overlap); O32422|SYH_STAAU|hiss from Staphylococcus aureus (420 aa), FASTA scores: opt: 1135, E(): 7.4e-59, (44.9% identity in 412 aa overlap); P04804|SYH_ECOLI|hiss|B2514 from Escherichia coli strain K12 (423 aa), FASTA scores: opt: 1099, E(): 9.4e-57, (43.9% identity in 417 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to class-II aminoacyl-tRNA synthetase family. P9WFV5 No information pathways P67483 P9WFV5 GO:0005737,GO:0004821,GO:0006427,GO:0005524 6.1.1.21 P9WFV5 NC_000962.3 Mycobrowser_v5 CDS 2906089 2906763 . - 0 Rv2581c Rv2581c Involved in glyoxal pathway. Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid [catalytic activity: (S)-(2-hydroxyacyl)glutathione + H(2)O = glutathione + a 2-hydroxy acid anion]. Possible glyoxalase II (hydroxyacylglutathione hydrolase) (GLX II) Rv2581c, (MTCY227.20), len: 224 aa. Possible glyoxalase II, equivalent to Q49649|YP81_MYCLE|ML0493|MLCB1259.11|B1177_C3_247 hypothetical 23.9 KDA protein from Mycobacterium leprae (218 aa), FASTA scores: opt: 1264, E(): 7.8e-73, (82.0% identity in 222 aa overlap). Also highly similar to Q9KXP1|SC9C5.33c possible hydrolase from Streptomyces coelicolor (235 aa), FASTA scores: opt: 654, E(): 2.9e-34, (46.8% identity in 220 aa overlap); and similar to Q9CI24|YFCI hypothetical protein from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (210 aa), FASTA scores: opt: 360, E(): 9.9e-16, (35.0% identity in 217 aa overlap); AAK75726|SP1646 metallo-beta-lactamase superfamily protein from Streptococcus pneumoniae (209 aa), FASTA scores: opt: 320, E(): 3.3e-13, (35.85% identity in 198 aa overlap); AAK80229|CAC2272 predicted Zn-dependent hydrolase of metallo-beta-lactamase superfamily from Clostridium acetobutylicum (199 aa), FASTA scores: opt: 282, E(): 8e-11, (32.7% identity in 217 aa overlap); etc. Equivalent to AAK46971 from Mycobacterium tuberculosis strain CDC1551 (246 aa) but shorter 22 aa. Belongs to the glyoxalase II family. Cofactor: binds two zinc ions. P9WMW3 No virulence, detoxification, adaptation P9WMW3 GO:0046872,GO:0016787 3.-.-.- P9WMW3 NC_000962.3 Mycobrowser_v5 CDS 2906814 2907740 . + 0 Rv2582 ppiB PPIases accelerate the folding of proteins [catalytic activity: cis-trans isomerization of proline imidic peptide bonds inoligopeptides]. Probable peptidyl-prolyl cis-trans isomerase B PpiB (cyclophilin) (PPIase) (rotamase) (peptidylprolyl isomerase) Rv2582, (MTCY227.19c), len: 308 aa. Probable ppiB (alternate gene name: ppi), cyclophilin (peptidyl-prolyl cis-trans isomerase), equivalent to P46697|PPIB_MYCLE|PPI|ML0492|MLCB1259.10c|B1177_F3_97 probable peptidyl-prolyl cis-trans isomerase B from Mycobacterium leprae (295 aa), FASTA scores: opt: 1423, E(): 1.3e-66, (72.2% identity in 295 aa overlap). Also similar to others e.g. Q9KJG8|PPIB peptidyl-prolyl cis-trans isomerase from Streptomyces lividans (277 aa), FASTA scores: opt: 485, E(): 3.2e-18, (38.35% identity in 292 aa overlap); Q9KXP0|SC9C5.34 peptidyl-prolyl cis-trans isomerase from Streptomyces coelicolor (277 aa), FASTA scores: opt: 483, E(): 4.1e-18, (38.35% identity in 292 aa overlap); Q9RT72|DR1893 peptidyl-prolyl cis-trans isomerase from Deinococcus radiodurans (350 aa), FASTA scores: opt: 296, E(): 2.2e-08, (29.0% identity in 276 aa overlap); etc. Belongs to the cyclophilin-type PPIase family. P9WHW1 No information pathways Q50639 P9WHW1 GO:0006457,GO:0003755 5.2.1.8 P9WHW1 NC_000962.3 Mycobrowser_v5 CDS 2939012 2939962 . - 0 Rv2611c Rv2611c Catalyzes the acylation of the 6-position of the mannose residue linked to position 2 of the myo-inositol in phosphatylinositol mono- and DI-mannosides. Probable acyltransferase Rv2611c, (MTCY01A10.22), len: 316 aa. Probable acyltransferase , equivalent to O07148|MLCL581.15c|ML0453 hypothetical 35.4 KDA protein from Mycobacterium leprae (320 aa), FASTA scores: opt: 1529, E(): 5e-90, (71.45% identity in 312 aa overlap); and equivalent to Q9F7Y8 putative acyltransferase from Mycobacterium smegmatis (303 aa), FASTA scores: opt: 1464, E(): 6.5e-86, (72.15% identity in 291 aa overlap) (see citation below). Also highly similar to Q9L283|SCL2.16c putative acyltransferase from Streptomyces coelicolor (311 aa), FASTA scores: opt: 810, E(): 2.8e-44, (47.7% identity in 302 aa overlap); and similar to other acyltransferases e.g. Q9F0N3 acyltransferase from Campylobacter jejuni (295 aa), FASTA scores: opt: 207, E(): 6.4e-06, (20.45% identity in 220 aa overlap); Q9K379 acyltransferase (lipid a biosynthesis acyltransferase) from Campylobacter jejuni (295 aa), FASTA scores: opt: 203, E(): 1.1e-05, (20.0% identity in 220 aa overlap); etc. P9WMB5 No lipid metabolism O06203 P9WMB5 GO:0016021,GO:0009244,GO:0005886,GO:0008415 2.3.1.- P9WMB5 NC_000962.3 Mycobrowser_v5 CDS 2907826 2910198 . - 0 Rv2583c relA Involved in the metabolism of PPGPP (at the first step). In eubacteria PPGPP (guanosine 3'-diphosphate 5-'diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of PPPGPP which is then hydrolysed to form PPGPP [catalytic activity: ATP + GTP = AMP + guanosine 3'-diphosphate 5-'triphosphate]. Probable GTP pyrophosphokinase RelA (ATP:GTP 3'- pyrophosphotransferase) (PPGPP synthetase I) ((P)PPGPP synthetase) (GTP diphosphokinase) Rv2583c, (MTCY227.18), len: 790 aa. Probable relA, GTP pyrophosphokinase, equivalent to Q49640|RELA_MYCLE|ML0491|MLCB1259.09|B1177_C1_168 probable GTP pyrophosphokinase from Mycobacterium leprae (787 aa), FASTA scores: opt: 4834, E(): 0, (93.4% identity in 790 aa overlap). Also highly similar to others e.g. O87331|RELA_CORGL|RELA|rel from Corynebacterium glutamicum (Brevibacterium flavum) (760 aa), FASTA scores: opt: 3375, E(): 1.6e-196, (67.0% identity in 758 aa overlap); O85709|RELA_STRAT from Streptomyces antibioticus (841 aa), FASTA scores: opt: 3209, E(): 1.9e-186, (63.85% identity in 786 aa overlap); Q9KDH1|RELA|BH1242 from Bacillus halodurans (728 aa), FASTA scores: opt: 2195,E(): 3.8e-125, (45.65% identity in 714 aa overlap); etc. Belongs to the RELA / spot family. P9WHG9 No intermediary metabolism and respiration P66014 P9WHG9 GO:0005525,GO:0008728,GO:0016597,GO:0015969,GO:0016301,GO:0005524 2.7.6.5 P9WHG9 NC_000962.3 Mycobrowser_v5 tRNA 2969497 2969568 . - 0 MTB000035 valT - tRNA-Val (CAC) valT, tRNA-Val, anticodon cac, length = 72 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 2910229 2910900 . - 0 Rv2584c apt Involved in purine salvage. Catalyses a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis [catalytic activity: AMP + pyrophosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate]. Adenine phosphoribosyltransferase Apt (APRT) (AMP diphosphorylase) (AMP pyrophosphorylase) (transphosphoribosidase) Rv2584c, (MTCY227.17), len: 223 aa. Probable apt, adenine phosphoribosyltransferase, similar, but longer in N-terminus, to others e.g. O87330|APT_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (185 aa), FASTA scores: opt: 524, E(): 1.3e-24, (50.95% identity in 159 aa overlap); P52561|APT_STRCO from Streptomyces coelicolor (182 aa), FASTA scores: opt: 503, E(): 2.3e-23, (51.85% identity in 164 aa overlap); P47956|APT_MUSPA|APRT from Mus pahari (Shrew mouse) (180 aa), FASTA scores: opt: 419, E(): 2.5e-18, (44.7% identity in 170 aa overlap); P07672|P09993|P77121|APT_ECOLI|B0469 from Escherichia coli strain K12 (183 aa), FASTA scores: opt: 393, E(): 1.9e-18, (42.6% identity in 162 aa overlap); etc. Contains PS00103 Purine/ pyrimidine phosphoribosyl transferases signature, and PS00144 Asparaginase / glutaminase active site signature 1. Belongs to the purine/pyrimidine phosphoribosyltransferase family. Nearest initiation codon indicated by homology is TTG at 17426 or GTG at 17465. P9WQ07 No intermediary metabolism and respiration Q50637 P9WQ07 GO:0006168,GO:0005737,GO:0006166,GO:0003999 2.4.2.7 P9WQ07 NC_000962.3 Mycobrowser_v5 CDS 2911004 2912677 . - 0 Rv2585c Rv2585c Unknown Possible conserved lipoprotein Rv2585c, (MT2662, MTCY227.16), len: 557 aa. Possible conserved lipoprotein precursor, possibly attached to the membrane by a lipid anchor and substrate-binding protein involved in transport, equivalent to Q49646|YP85_MYCLE|ML0489|MLCB1259.07|B1177_C2_197 hypothetical lipoprotein precursor from Mycobacterium leprae (555 aa), FASTA scores: opt: 2812, E(): 9.8e-158, (78.95% identity in 546 aa overlap); and C-terminus highly similar to C-terminus of Q49638|DCIAE|B1177_C1_166 DCIAE protein from Mycobacterium leprae (344 aa), FASTA scores: opt: 1177, E(): 7.4e-62, (78.6% identity in 229 aa overlap). Also similar in part to various proteins, principally substrate-binding proteins, e.g. O87329|DCIAE dipeptide-binding protein from Corynebacterium glutamicum (Brevibacterium flavum) (502 aa), FASTA scores: opt: 614, E(): 1.2e-28, (30.7% identity in 427 aa overlap); Q9AKR0|OPPA|CAC49261 putative oligopeptide uptake ABC transporter periplasmic solute-binding protein precursor from Rhizobium meliloti (Sinorhizobium meliloti) (532 aa), FASTA scores: opt: 209, E(): 7.7e-05, (22.85% identity in 460 aa overlap); P76128|YDDS_ECOLI|B1487|P77769|P76874 putative ABC transporter periplasmic binding protein from Escherichia coli strain K12 (516 aa), FASTA scores: opt: 182, E(): 0.0029, (20.0% identity in 315 aa overlap); etc. P9WL77 No cell wall and cell processes Q50636 P9WL77 GO:0006810,GO:0005215,GO:0005886 P9WL77 NC_000962.3 Mycobrowser_v5 CDS 2912683 2914011 . - 0 Rv2586c secF Involved in protein export. Part of the prokaryotic protein translocation apparatus which comprise SECA, SECB, SECD, SECE, SECF, SECG and SECY. Probable protein-export membrane protein SecF Rv2586c, (MT2663, MTCY227.15), len: 442 aa. Probable secF, protein-export membrane protein (integral membrane protein) (see citation below), equivalent to P38386|SECF_MYCLE|SECF|ML0488|MLCB1259.06|B1177_C3_239 protein-export membrane protein from Mycobacterium leprae (471 aa), FASTA scores: opt: 1910, E(): 2.9e-104, (72.15% identity in 456 aa overlap). Also similar to others e.g. Q9AE06|SECF from Corynebacterium glutamicum (Brevibacterium flavum) (403 aa), FASTA scores: opt: 1198, E(): 9.8e-63, (47.1% identity in 399 aa overlap); Q53956|SECF_STRCO|SCL2.05c from Streptomyces coelicolor (373 aa), FASTA scores: opt: 670, E(): 6.4e-32, (39.25% identity in 400 aa overlap); Q55611|SECF_SYNY3|SLR0775 from Synechocystis sp. strain PCC 6803 (315 aa), FASTA scores: opt: 416, E(): 3.8e-17, (33.8% identity in 296 aa overlap); etc. Belongs to the SECD/SECF family, SECF family. Part of the prokaryotic protein translocation apparatus which comprise SECA|Rv3240c, SECD|Rv2587c, SECE|Rv0638, SECF, SECG|Rv1440 and SECY|Rv0732. P9WGN9 No cell wall and cell processes Q50635 P9WGN9 GO:0016021,GO:0006886,GO:0005886,GO:0015628,GO:0055085,GO:0015627,GO:0015450 P9WGN9 NC_000962.3 Mycobrowser_v5 CDS 2915846 2916193 . - 0 Rv2588c yajC Thought to be involved in secretion apparatus. Probable conserved membrane protein secretion factor YajC Rv2588c, (MTCY227.13), len: 115 aa. Probable yajC, secretion factor, a conserved membrane protein (see Braunstein & Belisle 2000), equivalent to Q49647|YP88_MYCLE|ML0486|MLCB1259.04|B1177_C3_235 hypothetical 12.8 KDA protein from Mycobacterium leprae (114 aa), FASTA scores: opt: 499, E(): 2.7e-26, (77.0% identity in 100 aa overlap). Also similar to other proteins e.g. Q9AE08 hypothetical 13.5 KDA protein from Corynebacterium glutamicum (Brevibacterium flavum) (121 aa), FASTA scores: opt: 222, E(): 5e-08, (39.8% identity in 103 aa overlap); Q9L292|SCL2.07c putative secreted protein from Streptomyces coelicolor (169 aa), FASTA scores: opt: 203, E(): 1.2e-06, (32.05% identity in 106 aa overlap); Q9CDT0|YWAB unknown protein from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (110 aa), FASTA scores: opt: 150, E(): 0.0026, (30.85% identity in 94 aa overlap); etc. P9WL75 No cell wall and cell processes P65025 P9WL75 GO:0005576 NC_000962.3 Mycobrowser_v5 CDS 2916360 2917709 . + 0 Rv2589 gabT Involved in 4-aminobutyrate (GABA) degradation pathway [catalytic activity: 4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate]. 4-aminobutyrate aminotransferase GabT (gamma-amino-N-butyrate transaminase) (GABA transaminase) (glutamate:succinic semialdehyde transaminase) (GABA aminotransferase) (GABA-at) Rv2589, (MTCY227.12c), len: 449 aa. Probable gabT, 4-aminobutyrate aminotransferase, equivalent to P40829|GABT_MYCLE|ML0485|MLCB1259.03c|B1177_F2_67 4-aminobutyrate aminotransferase (446 aa), FASTA scores: opt: 2468, E(): 4.5e-141, (83.75% identity in 449 aa overlap). Also highly similar to others e.g. O86823|GABT from Streptomyces coelicolor (444 aa), FASTA scores: opt: 1832, E(): 8e-103, (63.9% identity in 443 aa overlap); AAK79395|CAC1427 from Clostridium acetobutylicum (445 aa), FASTA scores: opt: 1283, E(): 8.4e-70, (45.75% identity in 433 aa overlap); Q9KE66|BH0991 from Bacillus halodurans (443 aa), FASTA scores: opt: 1224, E(): 2.9e-66, (44.55% identity in 431 aa overlap); etc. Contains PS00600 Aminotransferases class-III pyridoxal-phosphate attachment site. Belongs to class-III of pyridoxal-phosphate-dependent aminotransferases. Cofactor: pyridoxal phosphate. P9WQ79 No intermediary metabolism and respiration P63504 P9WQ79 GO:0003867,GO:0009448,GO:0030170,GO:0047298 2.6.1.22,2.6.1.19 P9WQ79 NC_000962.3 Mycobrowser_v5 CDS 2917871 2921377 . + 0 Rv2590 fadD9 Function unknown, but involved in lipid degradation. Probable fatty-acid-CoA ligase FadD9 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv2590, (MTCY227.11c), len: 1168 aa. Probable fadD9, fatty-acid-CoA synthetase, highly similar to O69484|FADD9 (alias Q9CCT4|FADD9|ML0484 but longer 14 aa) putative acyl-CoA synthetase from Mycobacterium leprae (1174 aa), FASTA scores: opt: 5247, E(): 0, (68.0% identity in 1178 aa overlap). N-terminal (approximately 700 residues) similar to other long chain fatty acid ligases. And C-terminus highly similar to C-terminus of Q9XCF2|PSTB PSTB protein from Mycobacterium avium (2552 aa), FASTA scores: opt: 2083, E(): 8.4e-116, (40.8% identity in 1150 aa overlap) (and weak similarity on N-terminus). C-terminal part highly similar to polyketide synthases and peptides synthases (weak similarity on N-terminus) e.g. Q10896|Rv0101|MTCY251.20|NRP probable peptide synthetase from Mycobacterium tuberculosis (2512 aa), FASTA scores: opt: 1988, E(): 3.7e-110, (40.2% identity in 1181 aa overlap); etc. Contains PS00455 putative AMP-binding domain signature, and PS00061 Short-chain alcohol dehydrogenase family signature. Seems to belong to the ATP-dependent AMP-binding enzyme family, and to the short-chain dehydrogenases/reductases (SDR) family. Q50631 No lipid metabolism Q50631 Q50631 GO:0000036,GO:0048037,GO:0016874,GO:0008152,GO:0004043 6.2.1.- Q50631 NC_000962.3 Mycobrowser_v5 CDS 2924230 2924820 . - 0 Rv2593c ruvA forms a complex with RUVB. RUVA stimulates, in the presence of DNA, the weak ATPase activity of RUVB. The RUVA-RUVB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RUVAB is an helicase that mediates the holliday junction migration by localized denaturation and reanneling. Probable holliday junction DNA helicase RuvA Rv2593c, (MTCY227.08), len: 196 aa. Probable ruvA, Holliday junction binding protein (see citations below), equivalent to P40832|RUVA_MYCLE|ML0482|B1177_C2_188 holliday junction DNA helicase from Mycobacterium leprae (203 aa), FASTA scores: opt: 923, E(): 9.9e-50, (76.85% identity in 203 aa overlap). Also highly similar to others e.g. Q9L290|RUVA from Streptomyces coelicolor (201 aa) (201 aa), FASTA scores: opt: 549, E(): 8.2e-27, (47.55% identity in 204 aa overlap); Q9AE10|RUVA from Corynebacterium glutamicum (Brevibacterium flavum) (206 aa), FASTA scores: opt: 440, E(): 4e-20, (47.1% identity in 206 aa overlap); P08576|RUVA_ECOLI|B1861|Z2913|ECS2571 from Escherichia coli strains K12 and O157:H7 (203 aa), FASTA scores: opt: 312, E(): 2.8e-12, (34.85% identity in 201 aa overlap); etc. Belongs to the RuvA family. P9WGW3 No information pathways 2H5X,2ZTC,2ZTD,2ZTE P66744 P9WGW3 GO:0003677,GO:0006310,GO:0006281,GO:0009379,GO:0009432,GO:0009378,GO:0005524 3.6.4.12 P9WGW3 NC_000962.3 Mycobrowser_v5 CDS 2927990 2928391 . + 0 Rv2600 Rv2600 Unknown Probable conserved integral membrane protein Rv2600, (MTCY277.01c, MTV001.01), len: 133 aa. Probable conserved integral membrane protein, equivalent (but shorter 18 aa) to Q9K537|YQ00_MYCPA hypothetical protein RV2600 homolog from Mycobacterium paratuberculosis (151 aa), FASTA scores: opt: 543, E(): 4.2e-28, (62.9% identity in 132 aa overlap). Also some similarity with other hypothetical or membrane proteins e.g. Q9L090|SCC24.27c putative integral membrane protein from Streptomyces coelicolor (146 aa), FASTA scores: opt: 241, E(): 8.7e-09, (34.8% identity in 135 aa overlap); O58487|PH0773 hypothetical 15.0 KDA protein from Pyrococcus horikoshii (138 aa), FASTA scores: opt: 116, E(): 0.84, (34.35% identity in 96 aa overlap); etc. Equivalent to AAK46990 from Mycobacterium tuberculosis strain CDC1551 (152 aa) but shorter 19 aa. P9WFG5 No cell wall and cell processes P68915 P9WFG5 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2928388 2929959 . + 0 Rv2601 speE Involved in the biosynthesis of spermidine from arginine (at the fifth, last step). The activity is thought to be regulated mainly by the availability of decarboxylated S-adenosylmethionine [catalytic activity: S-adenosylmethioninamine + putrescine = 5'-methylthioadenosine + spermidine]. Probable spermidine synthase SpeE (putrescine aminopropyltransferase) (aminopropyltransferase) (SPDSY) Rv2601, (MTCI270.04c-MTV001.02), len: 523 aa. Probable speE, spermidine synthase, highly similar to many e.g. Q9L091|SCC24.26c from Streptomyces coelicolor (531 aa), FASTA scores: opt: 1493, E(): 1.3e-79, (48.45% identity in 514 aa overlap); Q9X8S2|SCH10.33c from Streptomyces coelicolor (554 aa), FASTA scores: opt: 1045, E(): 1.7e-53, (40.55% identity in 525 aa overlap); P09158|SPEE_ECOLI|B0121 from Escherichia coli strain K12 (287 aa), FASTA scores: opt: 368, E(): 2.9e-14, (30.5% identity in 272 aa overlap); etc. P9WGE5 No intermediary metabolism and respiration O33279 P9WGE5 GO:0004766,GO:0008295 2.5.1.16 P9WGE5 NC_000962.3 Mycobrowser_v5 CDS 2930805 2931560 . - 0 Rv2603c Rv2603c Function unknown Highly conserved protein Rv2603c, (MTCI270A.02), len: 251 aa. Highly conserved protein, equivalent to Q49645|YQ03_MYCLE|ML0475|U1177B|B1177_C2_181 hypothetical 26.6 KDA protein from Mycobacterium leprae (251 aa), FASTA scores: opt: 1514, E(): 2.2e-84, (92.45% identity in 251 aa overlap). Also highly similar to Q9L288|SCL2.11c hypothetical 26.8 KDA protein from Streptomyces coelicolor (250 aa), FASTA scores: opt: 1268, E(): 1.5e-69, (76.7% identity in 249 aa overlap); Q9AE12|YFCA hypothetical structural protein from Corynebacterium glutamicum (Brevibacterium flavum) (251 aa), FASTA scores: opt: 1231, E(): 2.6e-67, (72.9% identity in 251 aa overlap); O83487|Y474_TREPA|TP0474 hypothetical protein from Treponema pallidum (245 aa), FASTA scores: opt: 780, E(): 4.4e-40, (47.75% identity in 245 aa overlap); P24237|YEBC_ECOLI|B1864 protein YEBC from Escherichia coli strain K12 (246 aa), FASTA scores: opt: 776, E(): 7.6e-40, (47.8% identity in 249 aa overlap); etc. P9WGA5 No conserved hypotheticals P67177 P9WGA5 P9WGA5 NC_000962.3 Mycobrowser_v5 CDS 2931693 2932289 . - 0 Rv2604c snoP Possibly involved in the biosynthesis of pyridoxine/pyridoxal 5-phosphate biosynthesis Probable glutamine amidotransferase SnoP Rv2604c, (MTCY01A10.29, MTCI270A.01), len: 198 aa. Probable snoP, glutamine amidotransferase, equivalent (but shorter 21 aa) to Q49637|HISH|B1177_C1_149 HISH protein (belongs to the YFL060C/YAAE/HI1648 family) (alias Q9CCT5|ML0474 hypothetical protein 223 aa) from Mycobacterium leprae (219 aa), FASTA scores: opt: 1069, E(): 1.7e-60, (83.35% identity in 198 aa overlap). Also highly similar to hypothetical proteins or amidotransferases e.g. Q9L287|SCL2.12c hypothetical 21.5 KDA protein from Streptomyces coelicolor (202 aa), FASTA scores: opt: 702, E(): 2.3e-37, (56.75% identity in 192 aa overlap); P37528|YAAE_BACSU hypothetical 21.4 KDA protein from Bacillus subtilis (196 aa), FASTA scores: opt: 608, E(): 1.9e-31, (48.7% identity in 189 aa overlap); Q9KGN5|BH0023 amidotransferase from Bacillus halodurans (196 aa), FASTA scores: opt: 583, E(): 7.4e-30, (48.7% identity in 195 aa overlap); etc. Also some similarity with several proteins from Mycobacterium tuberculosis e.g. O06589|HIS5_MYCTU|Rv1602|MT1638|MTCY336.02c amidotransferase (206 aa), FASTA scores: opt: 154, E(): 0.00036, (30.6% identity in 193 aa overlap). Contains a Pfam match to entry PF01174 SNO glutamine amidotransferase family. Note possibly co-regulated with snzP (Rv2606c). P9WII7 No intermediary metabolism and respiration O06210 P9WII7 GO:0042823,GO:0016740,GO:0006543 2.6.-.- P9WII7 NC_000962.3 Mycobrowser_v5 CDS 2933171 2934070 . - 0 Rv2606c snzP Possibly involved in the biosynthesis of pyridoxine/pyridoxal 5-phosphate biosynthesis Possible pyridoxine biosynthesis protein SnzP Rv2606c, (MTCY01A10.27), len: 299 aa. Probable snzP, pyridoxine biosynthesis protein. Highly similar to O07145|YQ06_MYCLE|ML0450|MLCL581.12c possible pyridoxine biosynthesis protein from Mycobacterium leprae (307 aa), FASTA scores: opt: 1686, E(): 1.5e-95, (89.7% identity in 291 aa overlap). Also highly similar to several pyridoxine biosynthesis proteins and hypothetical proteins e.g. Q9L286|SCL2.13c hypothetical 32.2 KDA protein from Streptomyces coelicolor (303 aa), FASTA scores: opt: 1461, E(): 7.6e-82, (76.8% identity in 293 aa overlap); O14027|YEM4_SCHPO|SPAC29B12.04 putative stress-induced protein from Schizosaccharomyces pombe (Fission yeast) (296 aa), FASTA scores: opt: 1318, E(): 3.8e-73, (70.35% identity in 290 aa overlap); Q9UW83|PYROA protein involved in pyridoxine biosynthesis from Emericella nidulans (Aspergillus nidulans) (see citation below) (304 aa), FASTA scores: opt: 1288, E(): 2.6e-71, (67.9% identity in 302 aa overlap); etc. Contains Pfam match to entry PF01680, SOR_SNZ family. Contains PS01235 Uncharacterized protein family UPF0019 signature. Belongs to the SOR_SNZ family. Note possibly co-regulated with snoP (Rv2604c). P9WII9 No intermediary metabolism and respiration P60796 P9WII9 GO:0042823,GO:0016829 4.-.-.- P9WII9 NC_000962.3 Mycobrowser_v5 CDS 2934198 2934872 . + 0 Rv2607 pdxH Involved in biosynthesis of pyridoxine (vitamin B6) and pyridoxal phosphate. Oxidize PNP and PMP into pyridoxal 5'-phosphate (PLP)[catalytic activity: pyridoxamine 5'-phosphate + H(2)O + O(2) = pyridoxal 5'-phosphate + NH(3) + H(2)O(2)]. Probable pyridoxamine 5'-phosphate oxidase PdxH (PNP/PMP oxidase) (pyridoxinephosphate oxidase) (PNPOX) (pyridoxine 5'-phosphate oxidase) Rv2607, (MTCY01A10.26c), len: 224 aa. Probable pdxH, pyridoxinephosphate oxidase, equivalent to O33065|PDXH_MYCLE|ML2131|MLCB57.46 pyridoxamine 5'-phosphate oxidase from Mycobacterium leprae (219 aa), FASTA scores: opt: 1038, E(): 8.3e-61, (67.1% identity in 219 aa overlap). Also similar to others e.g. Q9I4S5|PDXH|PA1049 from Pseudomonas aeruginosa (215 aa), FASTA scores: opt: 608, E(): 1.1e-32, (49.55% identity in 218 aa overlap); Q9K3V7|SCD10.19c from Streptomyces coelicolor (234 aa), FASTA scores: opt: 600, E(): 3.9e-32, (42.3% identity in 234 aa overlap); P28225|PDXH_ECOLI|B1638 from Escherichia coli strain K12 (217 aa), FASTA scores: opt: 533, E(): 8.9e-28, (40.3% identity in 216 aa overlap); etc. Contains a match to Pfam entry PF01243 Pyridoxamine 5'-phosphate oxidase. Belongs to the pyridoxamine 5'-phosphate oxidase family. Cofactor: FMN. P9WIJ1 No intermediary metabolism and respiration 2A2J P65682 P9WIJ1 GO:0055114,GO:0004733,GO:0008615,GO:0010181 1.4.3.5 P9WIJ1 NC_000962.3 Mycobrowser_v5 CDS 2935046 2936788 . + 0 Rv2608 PPE42 Function unknown PPE family protein PPE42 Rv2608, (MTCY01A10.25c), len: 580 aa. PPE42, Member of the Mycobacterium tuberculosis PPE family, highly similar to many e.g. O06828|Rv1430|MTCY493.24c from Mycobacterium tuberculosis (528 aa), FASTA scores: opt: 1004, E(): 5.9e-48, (56.05% identity in 307 aa overlap). P9WHZ5 No PE/PPE Q79FC6 P9WHZ5 P9WHZ5 NC_000962.3 Mycobrowser_v5 CDS 2937865 2939001 . - 0 Rv2610c pimA Involved in the first mannosylation step in phosphatidylinositol mannoside biosynthesis (transfer of mannose residues onto PI, leading to the synthesis of phosphatidylinositol monomannoside). Alpha-mannosyltransferase PimA Rv2610c, (MTCY01A10.23), len: 378 aa. PimA, alpha-mannosyltransferase (see citations below), equivalent to O07147|MLCL581.14c|ML0452 putative glycosyltransferase from Mycobacterium leprae (374 aa), FASTA scores: opt: 2044, E(): 8.8e-118, (82.25% identity in 378 aa overlap). N-terminus (from aa 1 to 27) equivalent to Q9FY7 putative alpha-mannosyl transferase (fragment) from Mycobacterium smegmatis (27 aa), blastp scores: 57.4 bits (137), E(): 3e-8, Identities = 25/27 (92%), Positives = 27/27 (99%) (see citation below). Also highly similar to Q9L284|SCL2.15c putative sugar transferase from Streptomyces coelicolor (387 aa), FASTA scores: opt: 1222, E(): 1.8e-67, (52.95% identity in 376 aa overlap); and similar in part to various proteins e.g. Q9YA73|APE2066 long hypothetical N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein from Aeropyrum pernix (392 aa), FASTA scores: opt: 434, E(): 3e-19, (31.5% identity in 378 aa overlap); Q9UZA1|PAB0827 galactosyltransferase or LPS biosynthesis RFBU related protein from Pyrococcus abyssi (371 aa), FASTA scores: opt: 382, E(): 4.3e-16, (28.2% identity in 383 aa overlap); O26275|MTH173 LPS biosynthesis RFBU related protein from Methanothermobacter thermautotrophicus (382 aa), FASTA scores: opt: 372, E(): 1.8e-15, (28.4% identity in 391 aa overlap); etc. Shows also some similarity with O05313|Rv1212c|MTCI364.24c hypothetical 41.5 KDA protein from Mycobacterium tuberculosis (387 aa), FASTA scores: opt: 232, E(): 1.1e -07, (28.4% identity in 402 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). P9WMZ5 No lipid metabolism O06204 P9WMZ5 GO:0009405,GO:0043750,GO:0008654,GO:0005886,GO:0016021 2.4.1.57 P9WMZ5 NC_000962.3 Mycobrowser_v5 CDS 2940609 2941196 . - 0 Rv2613c Rv2613c Function unknown; but could be involved in lipid metabolism. Conserved protein Rv2613c, (MTCY01A10.20A), len: 195 aa. Conserved protein, equivalent to Q9CCU0|ML0455 hypothetical protein from Mycobacterium leprae (206 aa), FASTA scores: opt: 1074, E(): 7.4e-62, (84.7% identity in 196 aa overlap); and highly similar, but longer 18 aa, to O07150|MLCL581.17c hypothetical 20.7 KDA protein from Mycobacterium leprae (186 aa), FASTA scores: opt: 1038, E(): 1.4e-59, (89.7% identity in 175 aa overlap). Also highly similar to other hypothetical proteins (often Hit family member) e.g. Q9F7Z0 from Mycobacterium smegmatis (see citation below) (205 aa), FASTA scores: opt: 975, E(): 1.6e-55, (79.35% identity in 184 aa overlap); Q9L279|SCL2.20 from Streptomyces coelicolor (186 aa), FASTA scores: opt: 638, E(): 5.8e-34, (52.85% identity in 176 aa overlap); Q9YFX8|APE0122 from Aeropyrum pernix (184 aa), FASTA scores: opt: 515, E(): 4.4e-26, (45.9% identity in 159 aa overlap); etc. It seems the Rv2613c and downstream ORF Rv2612c|psgA1 are expressed from the same promoter (see citation below) and that Rv2613c should be involved in lipid metabolism. P9WMK9 No lipid metabolism 3ANO O06201 P9WMK9 GO:0005524,GO:0003877 2.7.7.53 P9WMK9 NC_000962.3 Mycobrowser_v5 CDS 2941189 2943267 . - 0 Rv2614c thrS Involved in translation mechanism [catalytic activity: ATP + L-threonine + tRNA(THR) = AMP + pyrophosphate + L-threonyl-tRNA(THR)]. Probable threonyl-tRNA synthetase ThrS (threonine-tRNA synthetase)(ThrRS) (threonine-tRNA ligase) Rv2614c, (MT2689, MTCY01A10.20), len: 692 aa. Probable thrS, threonyl-tRNA synthetase (Threonine--tRNA ligase), equivalent to O07151|SYT_MYCLE|THRS|ML0456|MLCL581.18c threonyl-tRNA synthetase from Mycobacterium leprae (702 aa), FASTA scores: opt: 3988, E(): 0, (84.05% identity in 702 aa overlap). Also highly similar to others e.g. Q9L278|THRS from Streptomyces coelicolor (658 aa), FASTA scores: opt: 1982, E(): 5.1e-114, (65.1% identity in 659 aa overlap); P56881|SYT_THETH|THRS from Thermus aquaticus (subsp. thermophilus) (659 aa), FASTA scores: opt: 1551, E(): 1.5e-87, (46.5% identity in 650 aa overlap); P00955|SYT_ECOLI from Escherichia coli (642 aa), FASTA scores: opt: 946, E(): 0, (40.7% identity in 612 aa overl ap); etc. Contains PS00339 Aminoacyl-transfer RNA synthetases class-II signature 2. Belongs to class-II aminoacyl-tRNA synthetase family. Cofactor: binds 1 zinc ion (by similarity). P9WFT5 No information pathways P67582 P9WFT5 GO:0005737,GO:0046872,GO:0004829,GO:0006435,GO:0005524 6.1.1.3 P9WFT5 NC_000962.3 Mycobrowser_v5 CDS 3769514 3769807 . - 0 Rv3355c Rv3355c Function unknown Probable integral membrane protein Rv3355c, (MTV004.12c), len: 97 aa. Probable integral membrane protein, equivalent to O32878|MLCB1779.16c|ML0675 hypothetical 9.6 KDA protein from Mycobacterium leprae (91 aa), FASTA scores: opt: 439, E(): 3.9e-23, (78.9% identity in 90 aa overlap). Identical, but with a gap, to O50377|Rv3346c|MTV004.02c hypothetical 8.9 KDA protein from Mycobacterium tuberculosis (85 aa), FASTA scores: opt: 413, E(): 2.1e-21, (85.55% identity in 97 aa overlap). Also some similarity to other proteins e.g. Q9K3J5|SC2A6.10 putative integral membrane protein from Streptomyces coelicolor (178 aa), FASTA scores: opt: 147, E(): 0.003, (31.25% identity in 80 aa overlap). O50384 No cell wall and cell processes O50384 O50384 NC_000962.3 Mycobrowser_v5 CDS 2948636 2949457 . + 0 Rv2622 Rv2622 Causes methylation Possible methyltransferase (methylase) Rv2622, (MTCY01A10.10c), len: 273 aa. Possible methyltransferase, similar in part to others e.g. AAK75664|SP1578 putative methyltransferase from Streptococcus pneumoniae (252 aa), FASTA scores: opt: 406, E(): 6.6e-18, (32.65% identity in 251 aa overlap); Q9F8B8 methyltransferase from Streptococcus agalactiae (254 aa), FASTA scores: opt: 381, E(): 2.3e-16, (31.75% identity in 252 aa overlap); Q9RJB6|SCF91.08 putative methyltransferase from Streptomyces coelicolor (231 aa), FASTA scores: opt: 159, E(): 0.0091, (33.1% identity in 151 aa overlap); etc. Also similar in part to several hypothetical proteins e.g. Q99YR0|SPY1582 hypothetical protein from Streptococcus pyogenes (251 aa), FASTA scores: opt: 397, E(): 2.3e-17, (36.3% identity in 248 aa overlap). I6XES4 No intermediary metabolism and respiration O06190 I6XES4 GO:0008168,GO:0008152 2.1.1.- I6XES4 NC_000962.3 Mycobrowser_v5 CDS 2949593 2950486 . + 0 Rv2623 TB31.7 Function unknown Universal stress protein family protein TB31.7 Rv2623, (MTCY01A10.09c), len: 297 aa. TB31.7, universal stress protein family protein, highly similar to hypothetical proteins from Mycobacterium tuberculosis e.g. Q10851|YK05_MYCTU|Rv2005c|MT2061|MTCY39.12 (295 aa), FASTA scores: opt: 1076, E(): 1.4e-60, (55.25% identity in 295 aa overlap); O53472|Rv2026c|MTV018.13c (294 aa), FASTA scores: opt: 988, E(): 4.8e-55, (51.5% identity in 295 aa overlap); Q10862|YJ96_MYCTU|Rv1996|MT2052|MTCY39.23c (317 aa), FASTA scores: opt: 688, E(): 4.1e-36, (45.1% identity in 315 aa overlap); etc. Also similar to several Streptomyces proteins e.g. Q9RIZ8|SCJ1.16c conserved hypothetical protein from Streptomyces coelicolor (294 aa), FASTA scores: opt: 407, E(): 2e-18, (32.65% identity in 303 aa overlap); and other bacterial hypothetical proteins e.g. Q9HPP5|VNG1536 from Halobacterium sp (147 aa), FASTA scores: opt: 180, E(): 0.00022, (31.65% identity in 139 aa overlap). Predicted possible vaccine candidate (See Zvi et al., 2008). Binds ATP. P9WFD7 No virulence, detoxification, adaptation 2JAX,3CIS O06189 P9WFD7 GO:0009405,GO:0006950,GO:0005524 P9WFD7 NC_000962.3 Mycobrowser_v5 CDS 2977231 2978658 . - 0 Rv2655c Rv2655c Unknown Possible PhiRv2 prophage protein Rv2655c, (MTCY441.24c), len: 475 aa. Hypothetical protein, possibly phiRv2 phage protein (putative primase-like protein) (see citation below). C-terminus similar to P22875|YXIS_SACER hypothetical 28.9 KDA protein (probably does not play a direct role in plasmid integration or excision) from Saccharopolyspora erythraea (Streptomyces erythraeus) plasmid pSE211 (263 aa), FASTA scores: opt: 389, E(): 2.7e-15, (33.45% identity in 269 aa overlap). Weak similarity in N-terminus to O06608|MTCY336.22|Rv1582c Probable phiRV1 phage protein from Mycobacterium tuberculosis (471 aa), FASTA scores: opt: 133, E(): 2.5, (36.0% identity in 75 aa overlap). I6Y1F0 No insertion seqs and phages P71952 I6Y1F0 I6Y1F0 NC_000962.3 Mycobrowser_v5 CDS 2957572 2958870 . + 0 Rv2631 Rv2631 Function unknown Conserved hypothetical protein Rv2631, (MTCY441.01, MTCY01A10.01c), len: 432 aa. Conserved hypothetical protein, highly similar to several conserved hypothetical proteins from various species e.g. O29399|AF0862 conserved hypothetical protein from Archaeoglobus fulgidus (482 aa), FASTA scores: opt: 1496, E(): 2.1e-80, (52.3% identity in 432 aa overlap) (has its N-terminus longer 30 aa); O27634|MTH1597 conserved protein from Methanothermobacter thermautotrophicus (488 aa), FASTA scores: opt: 1428, E(): 2.1e-76, (50.9% identity in 432 aa overlap); Q9YB37|APE1758 hypothetical 53.7 KDA protein APE1758 from Aeropyrum pernix (483 aa), FASTA scores: opt: 1422, E(): 4.6e-76, (49.3% identity in 432 aa overlap) (has its N-terminus longer 30 aa); etc. Equivalent to AAK47022 from Mycobacterium tuberculosis strain CDC1551 (432 aa). 3' part extended since first submission (+175 aa). P9WGW5 No conserved hypotheticals P71930 P9WGW5 P9WGW5 NC_000962.3 Mycobrowser_v5 CDS 2985283 2985753 . + 0 Rv2669 Rv2669 Acetylation, substrate unknown GCN5-related N-acetyltransferase Rv2669, (MTCY441.38), len: 156 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Similarity to several proteins e.g. Q9A6M0|CC2073 acetyltransferase (GNAT family) from Caulobacter crescentus (178 aa), FASTA scores: opt: 242, E(): 1.2e-09, (30.9% identity in 165 aa overlap); Q99RQ8|SA2159 hypothetical protein similar to transcription repressor of sporulation, septation and degradation paiA from Staphylococcus aureus subsp. aureus N315 (171 aa), FASTA scores: opt: 214, E(): 9.8e-08, (27.5% identity in 160 aa overlap); BAB58531|SAV2369 hypothetical 20.1 KDA protein from Staphylococcus aureus subsp. aureus Mu50 (171 aa), FASTA scores: opt: 214, E(): 9.8e-08, (27.5% identity in 160 aa overlap); P21340|PAIA_BACSU|O32112 protease synthase and sporulation from Bacillus subtilis (171 aa), FASTA scores: opt: 209, E(): 2.1e-07, (22.85% identity in 162 aa overlap); etc. P9WQG5 No intermediary metabolism and respiration P63425 P9WQG5 GO:0008152,GO:0008080 2.3.1.- P9WQG5 NC_000962.3 Mycobrowser_v5 CDS 2963586 2964242 . + 0 Rv2637 dedA Unknown Possible transmembrane protein DedA Rv2637, (MTCY441.07), len: 218 aa. Possible dedA, transmembrane protein, equivalent to Q49642|YQ37_MYCLE|ML0467|MLCL581.27|B1177_C2_172/B1177_C1_140 hypothetical 23.1 KDA protein (potential integral membrane protein, belongs to the DedA family) from Mycobacterium leprae (214 aa), FASTA scores: opt: 1160, E(): 4.4e-64, (82.75% identity in 209 aa overlap); and O69601|Y364_MYCLE|ML0287|MLCB4.30 hypothetical protein (potential integral membrane protein) (222 aa), FASTA scores: opt: 292, E(): 6.6e-11, (32.25% identity in 189 aa overlap). Also highly similar to other membrane proteins e.g. CAC42863|SCBAC36F5.27c putative integral membrane from Streptomyces coelicolor (211 aa), FASTA scores: opt: 837, E(): 2.6e-44, (59.2% identity in 201 aa overlap); Q55705|Y232_SYNY3|SLR0232 potential integral membrane protein from Synechocystis sp. strain PCC 6803 (218 aa), FASTA scores: opt: 415, E(): 1.9e-18, (37.85% identity in 206 aa overlap); Q9RV63|DR1167 DEDA protein from Deinococcus radiodurans (200 aa); P09548|DEDA_ECOLI|B2317|Z3579|ECS3201 DEDA protein (DSG-1 protein) from Escherichia coli strains K12 and O157:H7 (219 aa), blast scores: 178, E(): 1.8e-13, Identities = 53/175 (30%); etc. Also similar to O06314|Y364_MYCTU|Rv0364|MT0380|MTCY13E10.26 hypothetical 24.5 KDA protein (potential integral membrane protein) from Mycobacterium tuberculosis (227 aa), FASTA scores: opt: 293, E(): 5.8e-11, (35.85% identity in 184 aa overlap). Belongs to the DedA family. P9WP07 No cell wall and cell processes P63911 P9WP07 GO:0005886,GO:0016021 P9WP07 NC_000962.3 Mycobrowser_v5 CDS 2965026 2965358 . - 0 Rv2639c Rv2639c Unknown Probable conserved integral membrane protein Rv2639c, (MTCY441.09c), len: 110 aa. Probable conserved integral membrane protein, highly similar to many bacterial hypothetical or membrane proteins e.g. Q9X889|YE14_STRCO|SCE15.14 potential integral membrane protein from Streptomyces coelicolor (112 aa), FASTA scores: opt: 597, E(): 3.1e-31, (73.15% identity in 108 aa overlap); Q55939|Y793_SYNY3|SLL0793 potential integral membrane protein from Synechocystis sp. strain PCC 6803 (108 aa), FASTA scores: opt: 341, E(): 4.9e-15, (51.4% identity in 109 aa overlap); O31553|YFJF_BACSU potential integral membrane protein from Bacillus subtilis (109 aa), FASTA scores: opt: 334, E(): 1.4e-14, (47.5% identity in 109 aa overlap); etc. P9WFN9 No cell wall and cell processes P67146 P9WFN9 GO:0005886,GO:0016021 P9WFN9 NC_000962.3 Mycobrowser_v5 CDS 2965478 2965837 . - 0 Rv2640c Rv2640c Involved in transcriptional mechanism. Possible transcriptional regulatory protein (probably ArsR-family) Rv2640c, (MTCY441.10c), len: 119 aa. Possible transcriptional regulator, arsR family, highly similar to many e.g. Q9L1V5|SC4A9.07 putative ArsR-family transcriptional regulator from Streptomyces coelicolor (117 aa), FASTA scores: opt: 261, E(): 5.6e-10, (47.75% identity in 103 aa overlap); Q9X8X8|SCH35.28c putative transcriptional regulator from Streptomyces coelicolor (122 aa), FASTA scores: opt: 252, E(): 2.2e-09, (37.05% identity in 116 aa overlap); Q9L220|SC1A2.21 putative ArsR-family transcriptional from Streptomyces coelicolor (119 aa), FASTA scores: opt: 252, E(): 2.2e-09, (37.05% identity in 116 aa overlap); P77295|YGAV_ECOLI|B2667 hypothetical transcriptional regulator from Escherichia coli strain K12 (99 aa), FASTA scores: opt: 156, E(): 0.0023, (34.1% identity in 88 aa overlap); etc. Also similar to upstream ORF P71941|Rv2642|MTCY441.12 putative transcriptional regulatory protein from Mycobacterium tuberculosis (126 aa), FASTA scores: opt: 237, E(): 2e-08, (38.55% identity in 109 aa overlap). Contains helix-turn-helix motif at aa 59-80 (Score 1166, +3.16 SD). Belongs to the ArsR family of transcriptional regulators. I6Y1A7 No regulatory proteins P71939 I6Y1A7 GO:0006355,GO:0006350,GO:0003700,GO:0005622 I6Y1A7 NC_000962.3 Mycobrowser_v5 CDS 2966910 2968406 . + 0 Rv2643 arsC Involved in transport of arsenic compounds across the membrane (export): arsenic resistance by an export mechanism. Responsible for the translocation of the substrate across the membrane. Probable arsenic-transport integral membrane protein ArsC Rv2643, (MTCY441.13), len: 498 aa. Probable arsC, arsenical resistance transport integral membrane protein, highly similar or similar to others e.g. Q9L1X4|SC3D9.05 possible arsenic resistance membrane transport protein from Streptomyces coelicolor (368 aa), FASTA scores: opt: 1729, E(): 2.2e-96, (74.3% identity in 358 aa overlap); Q9X8Y0|SCH35.26 putative heavy metal resistance membrane protein from Streptomyces coelicolor (369 aa), FASTA scores: opt: 1729, E(): 2.2e-96, (73.8% identity in 359 aa overlap); Q06598|ACR3_YEAST|ACR3|YPR201W|P9677.2 arsenical-resistance protein from Saccharomyces cerevisiae (Baker's yeast) (404 aa), FASTA scores: opt: 591, E(): 4e-28, (36.6% identity in 380 aa overlap); etc. Belongs to the ACR3 family. I6X4W4 No cell wall and cell processes P71942 I6X4W4 GO:0016021,GO:0006470,GO:0004725,GO:0006814,GO:0008508 I6X4W4 NC_000962.3 Mycobrowser_v5 CDS 2979691 2980818 . - 0 Rv2659c Rv2659c Sequence integration. Integrase is necessary for integration of a phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome. Probable PhiRv2 prophage integrase Rv2659c, (MTCY441.28c), len: 375 aa. Probable integrase, phiRv2 phage protein: putative member of the phage integrase family of tyrosine recombinases (see Hatfull 2000), highly similar to others e.g. P22884|VINT_BPML5|33|int from Mycobacteriophage L5 (371 aa), FASTA scores: opt: 836, E(): 1.2e-44, (39.0% identity in 372 aa overlap); Q38361|VINT_BPMD2|33|int from Mycobacteriophage D29 (333 aa), FASTA scores: opt: 786, E(): 1.4e-41, (40.55% identity in 338 aa overlap); etc. Seems belongs to the 'phage' integrase family. P9WMB3 No insertion seqs and phages P71956 P9WMB3 GO:0015074,GO:0006310,GO:0003677 P9WMB3 NC_000962.3 Mycobrowser_v5 CDS 2985731 2986840 . - 0 Rv2670c Rv2670c Function unknown Conserved hypothetical protein Rv2670c, (MTCY441.39c), len: 369 aa. Conserved hypothetical protein, equivalent, but longer 164 aa, to O05683|MLC1351.22c hypothetical 17.3 KDA protein from Mycobacterium leprae (160 aa), FASTA scores: opt: 847, E(): 1.2e-45, (82.4% identity in 159 aa overlap). And highly similar to Q9X824|SC9B1.04c putative ATP/GTP-binding integral membrane protein from Streptomyces coelicolor (350 aa), FASTA scores: opt: 1169, E(): 2e-65, (56.85% identity in 343 aa overlap); and Q9RWB0|DR0759 conserved hypothetical protein from Deinococcus radiodurans (351 aa), FASTA scores: opt: 859, E(): 4e-46, (45.9% identity in 331 aa overlap). Also some similarity with other proteins e.g. P46442|YHCM_ECOLI|AAG58360|BAB37528 hypothetical protein from Escherichia coli strains K12 and O157:H7 (375 aa), FASTA scores: opt: 237, E(): 2.1e-07, (28.0% identity in 325 aa overlap); Q9JRK2|NMA1520|NMB1306 putative nucleotide-binding protein from Neisseria meningitidis (serogroup a and B) (383 aa), FASTA scores: opt: 221, E(): 2.1e-06, (27.8% identity in 356 aa overlap); Q9HVX7|PA4438 hypothetical protein from Pseudomonas aeruginosa (364 aa), FASTA scores: opt: 211, E(): 8.5e-06, (28.9% identity in 353 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). I6Y1G3 No conserved hypotheticals P71967 I6Y1G3 GO:0005524 I6Y1G3 NC_000962.3 Mycobrowser_v5 CDS 3001983 3003269 . + 0 Rv2685 arsB1 Thought to be involved in transport of arsenical compounds across the membrane (export): arsenic resistance by an export mechanism. Responsible for the translocation of the substrate across the membrane. Probable arsenic-transport integral membrane protein ArsB1 Rv2685, (MTCY05A6.06), len: 428 aa. Probable arsB1, arsenic-transport integral membrane protein, equivalent to P46838|AG45_MYCLE|ML1036 46 KDA probable integral membrane protein (antigen 45, a transmembrane protein related to arsenical pumps) from Mycobacterium leprae (429 aa), FASTA scores: opt: 2048, E(): 7.3e-120, (74.25% identity in 427 aa overlap); and downstream ORF O07186|YQ84_MYCTU|ARSA|Rv2684|MT2758|MTCY05A6.05 probable integral membrane protein ARSA from Mycobacterium tuberculosis (429 aa), FASTA scores: opt: 2154, E(): 1.9e-126, (76.8% identity in 427 aa overlap). Also highly similar to other proteins e.g. O59575|PH1912 hypothetical 46.0 KDA protein from Pyrococcus horikoshii (424 aa), FASTA scores: opt: 1075, E(): 1.9e-59, (43.55% identity in 427 aa overlap); Q9UY19|PAB1107 transport protein from Pyrococcus abyssi (425 aa), FASTA scores: opt: 1062, E(): 1.3e-58, (41.8% identity in 428 aa overlap); Q9KDI2|BH1231 hypothetical 46.0 KDA protein from Bacillus halodurans (428 aa), FASTA scores: opt: 993, E(): 2.4e-54, (39.55% identity in 430 aa overlap); etc. Belongs to the NADC/P/PHO87 family of transporters, P subfamily. Note that previously known as arsB. P9WPD7 No cell wall and cell processes O07187 P9WPD7 GO:0015137,GO:0015746,GO:0016021,GO:0005886,GO:0055085,GO:0015105 P9WPD7 NC_000962.3 Mycobrowser_v5 CDS 2989291 2990592 . + 0 Rv2673 aftC Involved in the biosynthesis of the mycobacterial cell wall arabinan Possible arabinofuranosyltransferase AftC Rv2673, (MTCY441.42), len: 433 aa. Possible aftC, arabinofuranosyltransferase (See Birch et al., 2008). Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). Possible conserved integral membrane protein, equivalent to MLC1351.25|ML1338 possible conserved integral membrane protein from Mycobacterium leprae (440 aa), FASTA scores: opt: 2410, E(): 5.3e-143, (82.05% identity in 434 aa overlap); and showing some similarity with Q9CBX0|ML1504 probable conserved membrane protein from Mycobacterium leprae (430 aa), FASTA scores: opt: 159, E(): 0.014, (24.4% identity in 340 aa overlap). Also similar to Q53873|SC6G4.11 putative integral membrane protein from Streptomyces coelicolor (411 aa), FASTA scores: opt: 383, E(): 1.4e-16, (29.6% identity in 422 aa overlap); and with weak similarity with P71061|YVFB hypothetical protein from Bacillus subtilis (396 aa), FASTA scores: opt: 136, E(): 0.36, (24.35% identity in 279 aa overlap); and BAB60134|TVG1014811 hypothetical protein from Thermoplasma volcanium (695 aa), FASTA scores: opt: 133, E(): 0.85, (26.45% identity in 280 aa overlap). Shows also some similarity with O06557|Rv1159|MTCI65.26 hypothetical 47.1 KDA protein from Mycobacterium tuberculosis (431 aa), FASTA scores: opt: 149, E(): 0.059, (22.45% identity in 410 aa overlap); and O53515|Rv2181|MTV021.14 putative membrane protein from Mycobacterium tuberculosis (427 aa), FASTA scores: opt: 129, E(): 1, (24.8% identity in 367 aa overlap). P9WMZ7 No cell wall and cell processes P71970 P9WMZ7 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2992634 2993992 . - 0 Rv2677c hemY Involved in heme and porphyrin biosynthesis (at the penultimate step). Catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX [catalytic activity: protoporphyrinogen-IX + O(2) = protoporphyrin-IX + H(2)O(2)]. Probable protoporphyrinogen oxidase HemY (protoporphyrinogen-IX oxidase) (protoporphyrinogenase) (PPO) Rv2677c, (MT2751, MTV010.01c), len: 452 aa. Probable hemY, protoporphyrinogen oxidase, equivalent to Q50008|PPOX_MYCLE|HEMY|ML1044 protoporphyrinogen oxidase from Mycobacterium leprae (451 aa), FASTA scores: opt: 2211, E(): 8.8e-118, (75.4% identity in 455 aa overlap). Also similar to others e.g. Q9RV99|DR1130 from Deinococcus radiodurans (462 aa), FASTA scores: opt: 523, E(): 2.7e-22, (29.8% identity in 453 aa overlap); O32434|PPOX_PROFR|HEMY from Propionibacterium freudenreichii shermanii (527 aa), FASTA scores: opt: 344, E(): 4e-12, (32.1% identity in 495 aa overlap); P32397|PPOX_BACSU|HEMY|HEMG from Bacillus subtilis (470 aa), FASTA scores: opt: 305, E(): 5.9e-10, (26.8% identity in 463 aa overlap); etc. Belongs to the protoporphyrinogen oxidase family. Cofactor: contains one FAD per homodimer. P9WMP1 No intermediary metabolism and respiration P0A5A7 P9WMP1 GO:0006783,GO:0055114,GO:0004729,GO:0005737 1.3.3.4 P9WMP1 NC_000962.3 Mycobrowser_v5 CDS 2993989 2995062 . - 0 Rv2678c hemE Involved in porphyrin biosynthesis [catalytic activity: uroporphyrinogen III = coproporphyrinogen + 4 CO(2)]. Probable uroporphyrinogen decarboxylase HemE (uroporphyrinogen III decarboxylase) (URO-D) (UPD) Rv2678c, (MTV010.02c), len: 357 aa. Probable hemE, uroporphyrinogen decarboxylase, equivalent to P46809|DCUP_MYCLE|heme|ML1043 uroporphyrinogen decarboxylase from Mycobacterium leprae (357 aa), FASTA scores: opt: 2017, E(): 8.2e-111, (83.75% identity in 357 aa overlap). Also highly similar to many e.g. O69861|DCUP_STRCO|heme|SC1C3.19 from Streptomyces coelicolor (355 aa), FASTA scores: opt: 1165, E(): 5.6e-61, (58.15% identity in 349 aa overlap); P32395|DCUP_BACSU|heme from Bacillus subtilis (353 aa), FASTA scores: opt: 859, E(): 4.5e-43, (44.1% identity in 356 aa overlap); Q9RV96|DCUP_DEIRA|heme|DR1133 from Deinococcus radiodurans (344 aa), FASTA scores: opt: 850, E(): 1.5e-42, (43.0% identity in 349 aa overlap); etc. Equivalent to AAK47067 from Mycobacterium tuberculosis strain CDC1551 (372 aa) but shorter 15 aa. Contains PS00907 Uroporphyrinogen decarboxylase signature 2. Belongs to the uroporphyrinogen decarboxylase family. P9WFE1 No intermediary metabolism and respiration O53231 P9WFE1 GO:0006779,GO:0004853,GO:0005737 4.1.1.37 P9WFE1 NC_000962.3 Mycobrowser_v5 CDS 2996105 2996737 . + 0 Rv2680 Rv2680 Function unknown Conserved protein Rv2680, (MTV010.04), len: 210 aa. Conserved protein, equivalent to Q50005|ML1041|U1764V hypothetical protein from Mycobacterium leprae (196 aa), FASTA scores: opt: 1136, E(): 9.7e-66, (83.95% identity in 193 aa overlap). Also similar to O69860|SC1C3.18c hypothetical 24.7 KDA protein from Streptomyces coelicolor (238 aa), FASTA scores: opt: 516, E(): 5.7e-26, (45.5% identity in 189 aa overlap); and similar in part to Q9I6V4|PA0178 probable two-component sensor from Pseudomonas aeruginosa (639 aa), FASTA scores: opt: 120, E(): 3.1, (33.05% identity in 115 aa overlap); and a few other proteins. Equivalent to AAK47069 from Mycobacterium tuberculosis strain CDC1551 (178 aa) but longer 32 aa; and N-terminus highly similar to N-terminus of AAK48352|MT3984 hypothetical 4.2 KDA protein from Mycobacterium tuberculosis strain CDC1551 (38 aa), FASTA scores: opt: 102, E(): 3.6, (62.05% identity in 29 aa overlap). Nucleotide position 2996194 in the genome sequence has been corrected, T:A resulting in V30V. O86317 No conserved hypotheticals O86317 O86317 O86317 NC_000962.3 Mycobrowser_v5 CDS 3010024 3010686 . + 0 Rv2692 ceoC Part of a potassium transport system. TRK system potassium uptake protein CeoC Rv2692, (MTCY05A6.13), len: 220 aa. CeoC (alternate gene names: trkA and trkB), TRK system potassium uptake protein (see citation below), highly similar to others e.g. O54141|SC2E9.16c from Streptomyces coelicolor (226 aa), FASTA scores: opt: 870, E(): 9.4e-48, (58.8% identity in 216 aa overlap); Q58505|TRKA_METJA|MJ1105 from Methanococcus jannaschii (218 aa), FASTA scores: opt: 361, E(): 9.7e-16, (29.8% identity in 218 aa overlap); O27333|TRKA_METTH|MTH1265 from Methanobacterium thermoautotrophicum (216 aa), FASTA scores: opt: 326, E(): 1.5e-13, (30.1% identity in 216 aa overlap); etc. Also similar to downstream orf O07193|CEOB|TRKA|Rv2691|MTCY05A6.12 TRK system potassium uptake protein from Mycobacterium tuberculosis (227 aa), FASTA scores: opt: 259, E(): 2.6e-09, (26.55% identity in 226 aa overlap). Contains a motif common to NAD+ binding pockets (see citation below). Belongs to the TrkA family. P9WFZ3 No cell wall and cell processes O07194 P9WFZ3 GO:0003824,GO:0008324,GO:0008152,GO:0006813 P9WFZ3 NC_000962.3 Mycobrowser_v5 CDS 3034909 3036102 . + 0 Rv2723 Rv2723 Unknown Probable conserved integral membrane protein Rv2723, (MTCY154.03), len: 397 aa. Probable conserved integral membrane protein, highly similar to others e.g. Q9Z503|SCC54.23c putative integral membrane export protein from Streptomyces coelicolor (333 aa), FASTA scores: opt: 883, E(): 2.4e-48, (46.4% identity in 332 aa overlap); Q9RD18|SCM1.25c putative integral membrane protein from Streptomyces coelicolor (316 aa), FASTA scores: opt: 865, E(): 3.1e-47, (47.55% identity in 324 aa overlap); P96554|Y319_MYXXA integral membrane protein (probable) from Myxococcus xanthus (319 aa), FASTA scores: opt: 626, E(): 3.4e-32, (34.65% identity in 323 aa overlap); P42601|YGJT_ECOLI|B3088 from Escherichia coli strain K12 integral membrane protein (probable) (321 aa), FASTA scores: opt: 541, E(): 7.7e-27, (35.1% identity in 279 aa overlap); etc. P9WG93 No cell wall and cell processes P0A614 P9WG93 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 3013683 3014147 . - 0 Rv2697c dut Involved in biosynthesis of thymidylate. This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA [catalytic activity: dUTP + H(2)O = dUMP + pyrophosphate]. Probable deoxyuridine 5'-triphosphate nucleotidohydrolase Dut (dUTPase) (dUTP pyrophosphatase) (deoxyuridine 5'-triphosphatase) (dUTP diphosphatase) (deoxyuridine-triphosphatase) Rv2697c, (MT2771, MTCY05A6.18c), len: 154 aa. Probable dut, deoxyuridine 5'-triphosphate nucleotidohydrolase (see citation below), equivalent to Q49992|DUT_MYCLE|ML1028 deoxyuridine 5'-triphosphate nucleotidohydrolase from Mycobacterium leprae (154 aa), FASTA scores: opt: 928, E(): 2.1e-51, (90.25% identity in 154 aa overlap). Also highly similar to others e.g. O54134|DUT_STRCO|SC2E9.09 from Streptomyces coelicolor (183 aa), FASTA scores: opt: 534, E(): 1.2e-26, (56.1% identity in 148 aa overlap); O66592|DUT_AQUAE|AQ_220 from Aquifex aeolicus (150 aa), FASTA scores: opt: 398, E(): 3.3e-18, (48.05% identity in 152 aa overlap); Q9X3X5|DUT_ZYMMO from Zymomonas mobilis (146 aa), FASTA scores: opt: 396, E(): 4.4e-18, (49.0% identity in 147 aa overlap); etc. Belongs to the dUTPase family. P9WNS5 No intermediary metabolism and respiration 1MQ7,1SIX,1SJN,1SLH,1SM8,1SMC,1SNF,2PY4,3H6D,3HZA,3I93,3LOJ P0A552 P9WNS5 GO:0046080,GO:0000287,GO:0004170 3.6.1.23 P9WNS5 NC_000962.3 Mycobrowser_v5 CDS 3015203 3015853 . + 0 Rv2700 Rv2700 Unknown Possible conserved secreted alanine rich protein Rv2700, (MTCY05A6.21), len: 216 aa. Possible secreted ala-rich protein, equivalent to Q4998|ML1025|U1764H possible secreted protein from Mycobacterium leprae (216 aa), FASTA scores: opt: 1198, E(): 1.2e-65, (82.4% identity in 216 aa overlap). Also showing some similarity with Q9AK75|2SCD60.08c conserved hypothetical protein from Streptomyces coelicolor (204 aa), FASTA scores: opt: 193, E(): 8.9e-05, (31.25% identity in 192 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). I6Y1I5 No cell wall and cell processes I6Y1I5 NC_000962.3 Mycobrowser_v5 CDS 3016858 3017655 . + 0 Rv2702 ppgK Catalyzes the phosphorylation of glucose using polyphosphate or ATP as the phosphoryl donor. GTP, UTP and CTP can replace ATP as phosphoryl donor [catalytic activity: (phosphate)(N) + D-glucose = (phosphate)(N-1) + D-glucose 6-phosphate]. Polyphosphate glucokinase PpgK (polyphosphate-glucose phosphotransferase) Rv2702, (MTCY05A6.23), len: 265 aa. PpgK, polyphosphate glucokinase (see citations below), equivalent, but shorter 60 aa, to Q49988|PPGK_MYCLE|ML1023|U1764FG polyphosphate glucokinase from Mycobacterium leprae (324 aa), FASTA scores: opt: 1411, E(): 5.6e-80, (82.8% identity in 262 aa overlap). Also highly similar (or just similar) to others e.g. Q9ADE8|PPGK from Streptomyces coelicolor (246 aa), FASTA scores: opt: 912, E(): 3e-49, (57.3% identity in 239 aa overlap); Q9AGV8|PPGK from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (277 aa), FASTA scores: opt: 890, E(): 7.5e-48, (57.75% identity in 239 aa overlap); P40184|GLK_STRCO|SC6E10.20c from Streptomyces coelicolor (317 aa), FASTA scores: opt: 233, E(): 3.2e-07, (31.3% identity in 163 aa overlap); etc. P9WIN1 No intermediary metabolism and respiration Q59568 P9WIN1 GO:0005737,GO:0047330,GO:0005524 2.7.1.63 P9WIN1 NC_000962.3 Mycobrowser_v5 CDS 3022461 3023432 . + 0 Rv2710 sigB The sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released. May control the regulons of stationary phase and general stress resistance. Seems to be regulated by sigh (Rv3223c product) and SIGE (Rv1221 product). Seems to regulate KATG|Rv1908c and the heat-shock response. RNA polymerase sigma factor SigB Rv2710, (MTCY05A6.31), len: 323 aa. SigB (formerly known as mysB), RNA polymerase sigma factor (see citations below), equivalent to Q59531|ML1014 RNA polymerase sigma factor from Mycobacterium leprae (319 aa), FASTA scores: opt: 1935, E(): 1.9e-109, (96.2% identity in 316 aa overlap). Also highly similar to others e.g. Q59553|MYSB from Mycobacterium smegmatis (319 aa), FASTA scores: opt: 1874, E(): 9.1e-106, (92.4% identity in 316 aa overlap); Q9ANT6|SIGB from Brevibacterium flavum (331 aa), FASTA scores: opt: 1525, E(): 9.9e-85, (78.9% identity in 303 aa overlap); Q60158|RPOV from Mycobacterium bovis (528 aa), FASTA scores: opt: 1246, E(): 9.3e-68, (62.85% identity in 315 aa overlap); etc. Contains sigma-70 factors family signatures 1 and 2 (PS00715 and PS00716). And contains possible helix-turn-helix motif at aa 282-303 (Score 1887, +5.61 SD). Belongs to the sigma-70 factor family. P9WGI5 No information pathways Q59563 P9WGI5 GO:0016987,GO:0003700,GO:0006352,GO:0006355 P9WGI5 NC_000962.3 Mycobrowser_v5 CDS 3833694 3834521 . - 0 Rv3415c Rv3415c Function unknown Conserved hypothetical protein Rv3415c, (MTCY78.14), len: 275 aa. Conserved hypothetical protein, equivalent to Q9CCV3|ML0383 hypothetical protein from Mycobacterium leprae (281 aa), FASTA scores: opt: 1278, E(): 4.2e-71, (73.5% identity in 279 aa overlap). Also some similarity with P71677|RIBD_MYCTU|RIBG|Rv1409|MT1453|MTCY21B4.26 riboflavin biosynthesis protein R (339 aa), FASTA scores: opt: 143, E(): 0.13, (28.25% identity in 184 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). I6YG27 No conserved hypotheticals I6YG27 I6YG27 NC_000962.3 Mycobrowser_v5 CDS 3025441 3026847 . + 0 Rv2713 sthA Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation [catalytic activity: NADPH + NAD(+) = NADP(+) + NADH]. Probable soluble pyridine nucleotide transhydrogenase SthA (STH) (NAD(P)(+) transhydrogenase [B-specific]) (nicotinamide nucleotide transhydrogenase) Rv2713, (MT2786, MTCY05A6.34), len: 468 aa. Probable sthA, soluble pyridine nucleotide transhydrogenase, highly similar to others e.g. Q983E2|MLR8366 from Rhizobium loti (Mesorhizobium loti) (481 aa), FASTA scores: opt: 1447, E(): 4.1e-78, (49.55% identity in 460 aa overlap); P27306|STHA_ECOLI|STH|UDHA|B3962 from Escherichia coli strain K12 (465 aa), FASTA scores: opt: 1267, E(): 1.7e-67, (43.05% identity in 462 aa overlap); O05139|STHA_PSEFL|STH from Pseudomonas fluorescens (463 aa), FASTA scores: opt: 1257, E(): 6.6e-67, (43.8% identity in 461 aa overlap); etc. Also highly similar to CAC46308|SMC00300 putative oxidoreductase protein from Rhizobium meliloti (Sinorhizobium meliloti) (467 aa), FASTA scores: opt: 1466, E(): 3e-79, (49.55% identity in 462 aa overlap). Shows some similarity to MTCY359.04, E(): 3.1e-08; MTCY210.05, E(): 3.4e-08. Contains ATP/GTP-binding site motif A (P-loop; PS00017). Belongs to the pyridine nucleotide-disulfide oxidoreductases class-I. Cofactor: FAD (by similarity). P9WHH5 No intermediary metabolism and respiration P66006 P9WHH5 GO:0003957,GO:0006739,GO:0045454,GO:0005737,GO:0055114,GO:0050660 1.6.1.1 P9WHH5 NC_000962.3 Mycobrowser_v5 CDS 3027065 3028039 . + 0 Rv2714 Rv2714 Function unknown Conserved alanine and leucine rich protein Rv2714, (MTCY05A6.35), len: 324 aa. Conserved ala-, leu-rich protein, equivalent to Q49847|ML1009|B2235_F1_6 hypothetical protein from Mycobacterium leprae (326 aa), FASTA scores: opt: 1881, E(): 5.8e-107, (89.7% identity in 320 aa overlap); and similar to Q49797|MLCB2533.03c|B2126_F1_36 hypothetical protein from Mycobacterium leprae (317 aa), FASTA scores: opt: 376, E(): 1.2e-15, (30.1% identity in 279 aa overlap); and Q9CC38|ML1306 hypothetical protein from Mycobacterium leprae (274 aa), FASTA scores: opt: 367, E(): 3.6e-15, (29.8% identity in 275 aa overlap). Also highly similar to Q9S2K6|SC7H2.11c hypothetical 34.2 KDA protein from Streptomyces coelicolor (312 aa), FASTA scores: opt: 770, E(): 1.4e-39, (40.9% identity in 286 aa overlap); and similar to Q9ADA5|SCI52.04 conserved hypothetical protein from Streptomyces coelicolor (333 aa), FASTA scores: opt: 386, E(): 3e-16, (29.05% identity in 296 aa overlap). Also similar to O33260|Rv2125|MTCY261.21 hypothetical protein from Mycobacterium tuberculosis (292 aa), FASTA scores: opt: 387, E(): 2.3e-16, (29.45% identity in 292 aa overlap). I6YA29 No conserved hypotheticals 2WAM O07213 I6YA29 I6YA29 NC_000962.3 Mycobrowser_v5 CDS 3031788 3032498 . + 0 Rv2720 lexA Involved in regulation of nucleotide excision repair and sos response. Represses a number of genes involved in the response to DNA damage (sos response), including RECA and LEXA. Has been shown to bind to the 14 bp palindromic sequence 5'-cgaacnnnngttcg-3'. In the presence of single-stranded DNA, RECA interacts with LEXA causing an autocatalytic cleavage which disrupts the DNA-binding part of LEXA, leading to derepression of the sos regulon and eventually DNA repair [catalytic activity: hydrolysis of ala-|-GLY bond in repressor LEXA]. Repressor LexA Rv2720, (MTCY05A6.41), len: 236 aa. LexA repressor (see citations below), equivalent to Q49848|LEXA_MYCLE|ML1003|B2235_F2_55 LEXA repressor from Mycobacterium leprae (217 aa), FASTA scores: opt: 1255, E(): 7.1e-70, (89.8% identity in 216 aa overlap). Also highly similar to others e.g. O69979|LEXA_STRCO|SC4H2.24c from Streptomyces coelicolor (234 aa), FASTA scores: opt: 1034, E(): 2.6e-56, (70.5% identity in 217 aa overlap); O86847|LEXA_STRCL from Streptomyces clavuligerus (239 aa), FASTA scores: opt: 1021, E(): 1.6e-55, (69.1% identity in 217 aa overlap); Q9KAD3|LEXA_BACHD from Bacillus halodurans (207 aa), FASTA scores: opt: 645, E(): 1.5e-32, (47.9% identity in 213 aa overlap); etc. Belongs to peptidase family S24; also known as the UMUD/LEXA family. Start changed since first submission (+19 aa). P9WHR7 No regulatory proteins Q50765 P9WHR7 GO:0006508,GO:0006281,GO:0006260,GO:0009432,GO:0045892,GO:0004252,GO:0006350,GO:0003677 3.4.21.88 P9WHR7 NC_000962.3 Mycobrowser_v5 CDS 3062816 3063634 . + 0 Rv2750 Rv2750 Function unknown; possibly involved in cellular metabolism. Probable dehydrogenase Rv2750, (MTV002.15), len: 272 aa. Probable dehydrogenase, highly similar to other dehydrogenases/reductases e.g. Q9L5X5|cox cholesterol oxidase from Nocardioides simplex (Arthrobacter simplex) (270 aa), FASTA scores: opt: 836, E(): 1.8e-43, (55.7% identity in 264 aa overlap); Q9RA05|LIMC carveol dehydrogenase from Rhodococcus erythropolis (277 aa), FASTA scores: opt: 792, E(): 8.6e-41, (48.55% identity in 274 aa overlap); Q9F5J1|SIM-NJ1|SIMD2 putative 3-keto-acyl-reductase from Streptomyces antibioticus (273 aa), FASTA scores: opt: 435, E(): 3.7e-19, (35.75% identity in 263 aa overlap); etc. Also highly similar to AAK44941MT0715 oxidoreductase (short-chain dehydrogenase/reductase family) from Mycobacterium tuberculosis strain CDC1551 (275 aa), FASTA scores: opt: 702, E(): 2.4e-35, (44.45% identity in 270 aa overlap); and similar to many other Mycobacterium tuberculosis dehydrogenases. P9WGS5 No intermediary metabolism and respiration O33292 P9WGS5 GO:0055114,GO:0016491 1.-.-.- P9WGS5 NC_000962.3 Mycobrowser_v5 CDS 3037427 3038914 . - 0 Rv2725c hflX Possibly a putative GTPase, modulating activity of HFLK and HFLC proteins. Probable GTP-binding protein HflX Rv2725c, (MTCY154.05c), len: 495 aa. Probable hflX (hfl for high frequency of lysogenization), GTP-binding protein ,equivalent to Q9CCC0|ML0997 (alias Q49843|HFLX but longer) possible ATP/GTP-binding protein from Mycobacterium leprae (488 aa), FASTA scores: opt: 2562, E(): 1.1e-133, (84.55% identity in 485 aa overlap). Also highly similar to many e.g. Q9XCC1 from Streptomyces fradiae (425 aa), FASTA scores: opt: 1280, E(): 3.2e-63, (57.7% identity in 423 aa overlap); P73965|HFLX|SLR1521 from Synechocystis sp. strain PCC 6803 (534 aa), FASTA scores: opt: 1028, E(): 2.8e-49, (44.7% identity in 414 aa overlap); P25519|HFLX_ECOLI|B4173 from Escherichia coli strain K12 (426 aa), FASTA scores: opt: 916, E(): 3.4e-43, (40.1% identity in 414 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). O33230 No intermediary metabolism and respiration O33230 O33230 GO:0016787,GO:0005622,GO:0005525 3.1.5.- O33230 NC_000962.3 Mycobrowser_v5 CDS 3039825 3040769 . - 0 Rv2727c miaA Catalyzes the first step in the biosynthesis of 2-methylthio-N6-(delta(2)-isopentenyl)-adenosine (ms[2]I[6]a]) adjacent to the anticodon of several tRNA species [catalytic activity: isopentenyl diphosphate + tRNA = pyrophosphate + tRNA containing 6-isopentenyladenosine]. Probable tRNA delta(2)-isopentenylpyrophosphate transferase MiaA (IPP transferase) (isopentenyl-diphosphate:tRNA isopentenyltransferase) (iptase) (IPPT) Rv2727c, (MTCY154.07c), len: 314 aa. Probable miaA, tRNA delta(2)-isopentenylpyrophosphate transferase, equivalent to P46811|MIAA_MYCLE|ML0995|B2235_C3_232 tRNA delta(2)-isopentenylpyrophosphate transferase from Mycobacterium leprae (311 aa), FASTA scores: opt: 1679, E(): 3.2e-89, (81.85% identity in 314 aa overlap). Also highly similar to many e.g. O69967|MIAA_STRCO|SC4H2.12 from Streptomyces coelicolor (312 aa), FASTA scores: opt: 1006, E(): 1.2e-50, (55.5% identity in 301 aa overlap); O31795|MIAA_BACSU from Bacillus subtilis (314 aa), FASTA scores: opt: 671, E(): 1.9e-31, (38.55% identity in 293 aa overlap);P16384|MIAA_ECOLI|TRPX|B4171 from Escherichia coli strain K12 and Shigella flexneri (316 aa), FASTA scores: opt: 565, E(): 2.3e-25, (35.2% identity in 307 aa overlap);etc. Contains PS00017 ATP/GTP-binding site motif A (P -loop). Belongs to the IPP transferase family. P9WJW1 No intermediary metabolism and respiration P65352 P9WJW1 GO:0016779,GO:0004811,GO:0008033,GO:0005524 2.5.1.75 P9WJW1 NC_000962.3 Mycobrowser_v5 CDS 3043026 3044378 . + 0 Rv2731 Rv2731 Function unknown Conserved alanine and arginine rich protein Rv2731, (MTCY174.11), len: 450 aa. Conserved ala-, arg-rich protein, highly similar in part to Q49849|B2235_F2_77 hypothetical protein from Mycobacterium leprae (266 aa), FASTA scores: opt: 368, E(): 1e-10, (73.5% identity in 83 aa overlap); and Q9KXN9|SC9C5.35 hypothetical 6.5 KDA protein (fragment) from Streptomyces coelicolor (58 aa), FASTA scores: opt: 214, E(): 0.00065, (51.7% identity in 58 aa overlap). Also similar to Q9L296|SCL2.01 hypothetical 37.4 KDA protein (fragment) from Streptomyces coelicolor (328 aa), FASTA scores: opt: 843, E(): 3.7e-33, (45.95% identity in 296 aa overlap) (but N-terminus shorter); and shows some similarity with other proteins e.g. Q26938 kinetoplast-associated protein (KAP) from Trypanosoma cruzi (1052 aa), FASTA scores: opt: 223, E(): 0.0022, (30.3% identity in 297 aa overlap). Start site chosen by RBS and to avoid overlap, although there are several other possible start sites further upstream. I6XF60 No conserved hypotheticals O33236 I6XF60 I6XF60 NC_000962.3 Mycobrowser_v5 CDS 3044986 3046524 . - 0 Rv2733c Rv2733c Unknown Conserved hypothetical alanine, arginine-rich protein Rv2733c, (MTCY154.13c), len: 512 aa. Conserved hypothetical ala-, arg-rich protein. Similar to other hypothetical proteins from a range of organisms e.g. Y195_MYCLE|Q49842 hypothetical 56.0 kDa protein b2235_c2_195 from Mycobacterium leprae (516 aa), FASTA scores: opt: 2689, E(): 0, (80.4% identity in 509 aa overlap). P9WK05 No conserved hypotheticals P67085 P9WK05 GO:0005737,GO:0046872,GO:0006400,GO:0016740,GO:0051539 2.-.-.- P9WK05 NC_000962.3 Mycobrowser_v5 CDS 3052023 3053189 . - 0 Rv2739c Rv2739c Function unknown; probably involved in cellular metabolism. Possible alanine rich transferase Rv2739c, (MTV002.04c), len: 388 aa. Possible ala-rich transferase, equivalent to Q49841|ML0985|MLCB33.02c|U2235C possible glycosyltransferase from Mycobacterium leprae (392 aa), FASTA scores: opt: 2112, E(): 5.1e-114, (80.95% identity in 388 aa overlap). Shows some similarity with other transferases e.g. Q9S1V2|SCJ4.21 putative glycosyl transferase from Streptomyces coelicolor (407 aa), FASTA scores: opt: 290, E(): 2e-09, (27.75% identity in 382 aa overlap); Q9RYI3|DRA0329 putative glycosyltransferase from Deinococcus radiodurans (418 aa), FASTA scores: opt: 267, E(): 4.3e-08, (29.05% identity in 396 aa overlap); P96560|GTFC glycosyltransferase from Amycolatopsis orientalis (409 aa), FASTA scores: opt: 253, E(): 2.7e-07, (27.75% identity in 418 aa overlap); etc. Equivalent to AAK47130 from Mycobacterium tuberculosis strain CDC1551 (420 aa) but shorter 32 aa. O33282 No intermediary metabolism and respiration O33282 O33282 GO:0005975,GO:0030259,GO:0016758,GO:0030246 2.-.-.- O33282 NC_000962.3 Mycobrowser_v5 CDS 3068189 3068464 . - 0 Rv2755c hsdS.1 Implicated in restriction/modification of DNA. Component of type I restriction/modification system. It's possible that the M and S subunits together form a methyltransferase (MTASE) that methylates two adenine residues in complementary strands of bipartite DNA recognition sequence. Possible type I restriction/modification system specificity determinant (fragment) HsdS.1 (S protein) Rv2755c, (MTV002.20c), len: 91 aa. Possible hsdS.1, fragment of type I restriction/modification system specificity determinant (S protein), similar to the N-terminus of other hsdS proteins e.g. O34140|HSDS from Klebsiella pneumoniae (439 aa), FASTA scores: opt: 303, E(): 2.1e-13, (46.65% identity in 90 aa overlap); P72419|sty|SBLI from Salmonella typhimurium (434 aa), FASTA scores: opt: 278, E(): 1.1e-11, (47.65% identity in 86 aa overlap); and Q9P9X9|XF2741 from Xylella fastidiosa (412 aa), FASTA scores: opt: 144, E(): 0.015, (31.7% identity in 82 aa overlap). Also some similarity with O33303|Rv2761c|MTV002.26c|HSDS possible type I restriction/modification system specificity determinant from Mycobacterium tuberculosis (364 aa), FASTA scores: opt: 145, E(): 0.012, (29.9% identity in 87 aa overlap). Note that previously known as hsdS'. I6XF84 No information pathways I6XF84 NC_000962.3 Mycobrowser_v5 CDS 3057251 3058063 . - 0 Rv2744c 35kd_ag Unknown Conserved 35 kDa alanine rich protein Rv2744c, (MTV002.09c), len: 270 aa. 35kd_ag, conserved ala-rich protein 35-kd antigen (see O'Connor et al., 1990). N-terminal part is equivalent to Q49840|MLCB33.06c|B2235_C2_187 hypothetical protein from Mycobacterium leprae (167 aa), FASTA scores: opt: 789, E(): 3.4e-35, (85.05% identity in 147 aa overlap); and C-terminal part equivalent to Q49845|MLCB33.05c|B2235_C3_214 hypothetical protein from Mycobacterium leprae (114 aa), FASTA scores: opt: 465, E(): 3.6e-18, (65.8% identity in 114 aa overlap); note that these two proteins from Mycobacterium leprae are adjacent. Shows some similarity with Q55707||Y617_SYNY3|SLL0617 hypothetical 28.9 KDA protein from Synechocystis sp. strain PCC 6803 (267 aa), FASTA scores: opt: 155, E(): 0.19, (23.4% identity in 252 aa overlap); and C-terminus of Q9L4N1|EMM M protein from Streptococcus equisimilis (592 aa), FASTA scores: opt: 165, E(): 0.11, (23.45% identity in 260 aa overlap). C-terminus also similar to AAK45945|MT1676 conserved hypothetical protein from Mycobacterium tuberculosis strain CDC1551 (85 aa), FASTA scores: opt: 159, E(): 0.047, (50.9% identity in 55 aa overlap). Predicted possible vaccine candidate (See Zvi et al., 2008). P9WHP5 No conserved hypotheticals P0C5C4 P9WHP5 GO:0005737 P9WHP5 NC_000962.3 Mycobrowser_v5 CDS 3058602 3059231 . - 0 Rv2746c pgsA3 Involved in acidic phospholipid biosynthesis. This protein probably catalyzes the committed step to the synthesis of the acidic phospholipids [catalytic activity: CDP-diacylglycerol + glycerol-3-phosphate = CMP + 3-(3-phosphatidyl)-glycerol 1-phosphate]. Probable PGP synthase PgsA3 (CDP-diacylglycerol--glycerol-3-phosphate 3- phosphatidyltransferase) (phosphatidylglycerophosphate synthase) Rv2746c, (MTV002.11c), len: 209 aa. Probable pgsA3, PGP synthase (see citation below), transmembrane protein, equivalent, but longer 19 aa, to Q49839|O08087|PGSA|ML0979 PGSA from Mycobacterium leprae (193 aa), FASTA scores: opt: 925, E(): 3.7e-53, (77.15% identity in 188 aa overlap). Also highly similar to O86813|PGSA phosphatidylglycerophosphate synthase from Streptomyces coelicolor (263 aa), FASTA scores: opt: 692, E(): 6.6e-38, (57.85% identity in 185 aa overlap) (has its N-terminus longer); and similar to others (generally with N-terminus shorter) e.g. Q99XI0|PGSA|SPY2196 phosphatidylglycerophosphate synthase from Streptococcus pyogenes (180 aa), FASTA scores: opt: 368, E(): 5.4e-17, (39.9% identity in 168 aa overlap); Q9ZE96|PGSA_RICPR|PGSA|RP049 CDP-diacylglycerol--glycerol-3-phosphate 3- phosphatidyltransferase from Rickettsia prowazekii (181 aa), FASTA scores: opt: 343, E(): 2.3e-15, (40.1% identity in 172 aa overlap); P06978|PGSA_ECOLI|PGSA|B1912|Z3000|ECS2650 CDP-diacylglycerol--glycerol-3-phosphate 3- phosphatidyltransferase from Escherichia coli strains K12 and O157:H7 (181 aa), FASTA scores: opt: 322, E(): 5.3e-14, (34.45% identity in 180 aa overlap); etc. Also some similarity to PGSA2|Rv1822|MTCY1A11.21c probable CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase from Mycobacterium tuberculosis (209 aa), FASTA score: (27.1% identity in 166 aa overlap). Contains PS00379 CDP-alcohol phosphatidyltransferases signature. Belongs to the CDP-alcohol phosphatidyltransferase class-I family. P9WPG3 No lipid metabolism O33288 P9WPG3 GO:0016021,GO:0008654,GO:0005886,GO:0008444 2.7.8.5 P9WPG3 NC_000962.3 Mycobrowser_v5 CDS 3068461 3070083 . - 0 Rv2756c hsdM Implicated in methylation of DNA. Component of type I restriction/modification system. It is possible that the M and S subunits together form a methyltransferase (MTASE) that methylates two adenine residues in complementary strands of bipartite DNA recognition sequence. Possible type I restriction/modification system DNA methylase HsdM (M protein) (DNA methyltransferase) Rv2756c, (MTV002.21c), len: 540 aa. Possible hsdM, type I restriction/modification system DNA methylase (M protein), highly similar to others e.g. Q9P9X8|XF2742 from Xylella fastidiosa (519 aa), FASTA scores: opt: 1613, E(): 1.9e-96, (52.3% identity in 543 aa overlap); O34139|HSDM from Klebsiella pneumoniae (539 aa), FASTA scores: opt: 1267, E(): 4.4e-74, (45.9% identity in 549 aa overlap); P72418|sty|SBLI|HSDM from Salmonella typhimurium (539 aa), FASTA scores: opt: 1263, E(): 8e-74, (45.7% identity in 549 aa overlap); etc. Possible alternative start site (GTG) overlapping with termination codon of previous ORF 90 bp upstream. Note that the corresponding endonuclease (M protein) does not appear to be present in Mycobacterium tuberculosis. O33298 No information pathways O33298 O33298 GO:0006306,GO:0008170,GO:0003677 2.1.1.- O33298 NC_000962.3 Mycobrowser_v5 CDS 3071546 3072640 . - 0 Rv2761c hsdS Implicated in restriction/modification of DNA. Component of type I restriction/modification system. It is thought that the M and S subunits together form a methyltransferase (MTASE) that methylates two adenine residues in complementary strands of bipartite DNA recognition sequence. Possible type I restriction/modification system specificity determinant HsdS (S protein) Rv2761c, (MTV002.26c), len: 364 aa. Possible hsdS, type I restriction/modification system specificity determinant (S protein), similar in part to other hsdS protein (S proteins) e.g. Q9P9X9|XF2741 from Xylella fastidiosa (412 aa), FASTA scores: opt: 252, E(): 7.4e-09, (24.95% identity in 401 aa overlap); N-terminus of Q9RC12 type I S-subunit from Lactobacillus delbrueckii (subsp. lactis) (389 aa), FASTA scores: opt: 232, E(): 1.4e-07, (28.1% identity in 185 aa overlap); N-terminus of P72419|sty|SBLI from Salmonella typhimurium (434 aa), FASTA scores: opt: 221, E(): 8e-07, (28.45% identity in 130 aa overlap); C-terminus of P17222|PRRB_ECOLI from Escherichia coli strain CTR5X (401 aa), FASTA scores: opt: 197, E(): 2.8e-05, (27.05% identity in 148 aa overlap); etc. Seems to belong to type-I restriction system S methylase family. I6YEB1 No information pathways O33303 I6YEB1 GO:0006304,GO:0003677 I6YEB1 NC_000962.3 Mycobrowser_v5 CDS 3074636 3075373 . + 0 Rv2765 Rv2765 Function unknown; probably involved in cellular metabolism. Probable alanine rich hydrolase Rv2765, (MTV002.30), len: 245 aa. Probable ala-rich hydrolase, similar to various hydrolases or hypothetical proteins e.g. Q9KYM6|SC9H11.13c putative hydrolase from Streptomyces coelicolor (251 aa), FASTA scores: opt: 630, E(): 1.4e-33, (43.1% identity in 246 aa overlap); Q9A5T9|CC2358 dienelactone hydrolase family protein from Caulobacter crescentus (286 aa), FASTA scores: opt: 592, E(): 4.5e-31, (38.45% identity in 242 aa overlap); Q9FCF1|2SCD46.33 putative hydrolase (dienelactone hydrolase family) from Streptomyces coelicolor (254 aa), FASTA scores: opt: 500, E(): 3.9e-25, (37.7% identity in 252 aa overlap); P73163|DLHH_SYNY3|SLL1298 putative carboxymethylenebutenolidase (dienelactone hydrolase) from Synechocystis sp. (strain PCC 6803) (246 aa), FASTA scores: opt: 276, E(): 1.3e-10, (26.95% identity in 230 aa overlap); etc. I6XF92 No intermediary metabolism and respiration O33307 I6XF92 GO:0008806 3.1.1.45 I6XF92 NC_000962.3 Mycobrowser_v5 CDS 3079309 3080457 . - 0 Rv2770c PPE44 Function unknown. May be involved in virulence. PPE family protein PPE44 Rv2770c, (MTV002.35c), len: 382 aa. PPE44, Member of the Mycobacterium tuberculosis PPE family, highly similar to many e.g. downstream ORF O33310|Rv2768c|MTV002.33c from M. tuberculosis (394 aa), FASTA scores: opt: 1135, E(): 2.2e-53, (62.15% identity in 391 aa overlap); and P96362|Rv1039c|MTCY10G2.10 from Mycobacterium tuberculosis (391 aa), FASTA scores: opt: 1010, E(): 1e-46, (55.95% identity in 395 aa overlap). Equivalent to AAK47159 from Mycobacterium tuberculosis strain CDC1551 (402 aa) but shorter 20 aa. Start changed since first submission (-20 aa). P9WHZ3 No PE/PPE Q79FA7 P9WHZ3 P9WHZ3 NC_000962.3 Mycobrowser_v5 CDS 3108416 3110065 . + 0 Rv2800 Rv2800 Function unknown; probably involved in cellular metabolism. Possible hydrolase Rv2800, (MTCY16B7.43c), len: 549 aa. Possible hydrolase, an esterase or an acylase. Similar, but longer in N-terminus, to esterases or acylases e.g. Q9L9D7|COCE cocaine esterase from Rhodococcus sp. MB1 'Bresler 1999' (574 aa), FASTA scores: opt: 510, E(): 3.1e-23, (33.6% identity in 571 aa overlap); Q9L3U2|STTE putative acylase from Streptomyces rochei (Streptomyces parvullus) (554 aa), FASTA scores: opt: 492, E(): 3.7e-22, (34.45% identity in 569 aa overlap); CAC49652|SMB21424 putative esterase or acylase protein from Rhizobium meliloti (Sinorhizobium meliloti) plasmid pSymB (578 aa), FASTA scores: opt: 405, E(): 7.1e-17, (34.45% identity in 569 aa overlap); etc. I6YEE6 No intermediary metabolism and respiration P71651 I6YEE6 GO:0008152,GO:0008239 3.-.-.- I6YEE6 NC_000962.3 Mycobrowser_v5 CDS 3081604 3082341 . - 0 Rv2773c dapB Involved in biosynthesis of diaminopimelate and lysine from aspartate semialdehyde (at the second step) [catalytic activity: 2,3,4,5-tetrahydrodipicolinate + NAD(P)(+) = 2,3-dihydrodipicolinate + NAD(P)H]. Dihydrodipicolinate reductase DapB (DHPR) Rv2773c, (MTV002.38c), len: 245 aa. DapB, dihydrodipicolinate reductase (see Pavelka et al., 1997), highly similar to many e.g. P40110|DAPB_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (248 aa), FASTA scores: opt: 1030, E(): 1.8e-58, (65.45% identity in 246 aa overlap); O86836|DAPB_STRCO|SC9A10.03 from Streptomyces coelicolor (250 aa), FASTA scores: opt: 997, E(): 2.3e-56, (61.15% identity in 247 aa overlap); P42976|DAPB_BACSU from Bacillus subtilis (267 aa), FASTA scores: opt: 608, E(): 1.7e-31, (45.95% identity in 209 aa overlap); P46829|DAPB_MYCBO from Mycobacterium bovis (see Cirillo et al., 1994) (271 aa), FASTA scores: opt: 505, E(): 6.3e-25, (36.2% identity in 246 aa overlap); etc. Belongs to the dihydrodipicolinate reductase family. P9WP23 No intermediary metabolism and respiration 1C3V,1P9L,1YL5,1YL6,1YL7 P72024 P9WP23 GO:0005737,GO:0019877,GO:0008839,GO:0055114 1.3.1.26 P9WP23 NC_000962.3 Mycobrowser_v5 CDS 3083374 3084303 . - 0 Rv2776c Rv2776c Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv2776c, (MTV002.41c), len: 309 aa. Probable oxidoreductase, similar to other oxidoreductases e.g. Q9KZ15|SC10B7.17 putative iron-sulfur oxidoreductase from Streptomyces coelicolor (364 aa), FASTA scores: opt: 846, E(): 1.2e-45, (46.75% identity in 308 aa overlap); O88034|SC5A7.28c iron-sulfur oxidoreductase beta subunit from Streptomyces coelicolor (313 aa), FASTA scores: opt: 745, E(): 2.3e-39, (41.45% identity in 316 aa overlap); P33164|PDR_BURCE|OPHA1 phthalate dioxygenase reductase from Burkholderia cepacia (Pseudomonas cepacia) (321 aa), FASTA scores: opt: 616, E(): 2.9e-31, (33.65% identity in 309 aa overlap); etc. Equivalent to AAK47165 from Mycobacterium tuberculosis strain CDC1551 (363 aa) but shorter 54 aa. Contains PS00197 2Fe-2S ferredoxins, iron-sulfur binding region signature and PS00063 Aldo/keto reductase family putative active site signature. Seems to belong to the 2FE2S plant-type ferredoxin family in the C-terminal section. O86347 No intermediary metabolism and respiration O86347 O86347 GO:0009055,GO:0046872,GO:0055114,GO:0016491,GO:0051537 1.-.-.- O86347 NC_000962.3 Mycobrowser_v5 CDS 3086820 3087935 . + 0 Rv2780 ald May play a role in cell wall synthesis as L-alanine is an important constituent of the peptidoglycan layer [catalytic activity: L-alanine + H(2)O + NAD(+) = pyruvate + NH(3) + NADH]. Secreted L-alanine dehydrogenase Ald (40 kDa antigen) (TB43) Rv2780, (MT2850, MTV002.45), len: 371 aa. Ald, secreted L-alanine dehydrogenase (40 kd antigen); equivalent to Q9CBV6|ALD|ML1532 L-alanine dehydrogenase from Mycobacterium leprae (371 aa), FASTA scores: opt: 2081, E(): 4e-115, (85.45% identity in 371 aa overlap). Also highly similar to others e.g. Q9S227|SCI51.13c from Streptomyces coelicolor (371 aa), FASTA scores: opt: 1575, E(): 2.3e-85, (66.05% identity in 371 aa overlap); Q9K827|BH3180 from Bacillus halodurans (371 aa), FASTA scores: opt: 1341, E(): 1.4e-71, (56.45% identity in 372 aa overlap); Q9RT70|DR1895 from Deinococcus radiodurans (390 aa), FASTA scores: opt: 1319, E(): 2.8e-70, (54.2% identity in 371 aa overlap); etc. Contains PS00836 and PS00837 Alanine dehydrogenase & pyridine nucleotide transhydrogenase signature 1 and 2. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WQB1 No intermediary metabolism and respiration 2VHV,2VHW,2VHX,2VHY,2VHZ,2VOE,2VOJ P30234 P9WQB1 GO:0005576,GO:0055114,GO:0000286 1.4.1.1 P9WQB1 NC_000962.3 Mycobrowser_v5 CDS 3089045 3090361 . - 0 Rv2782c pepR Function unknown; possibly hydrolyzes peptides and/or proteins Probable zinc protease PepR Rv2782c, (MTV002.47c), len: 438 aa. Probable pepR, protease/peptidase, equivalent to O32965|YR82_MYCLE|ML0855|MLCB22.26c hypothetical zinc protease from Mycobacterium leprae (445 aa), FASTA scores: opt: 2346, E(): 4.3e-146, (84.3% identity in 421 aa overlap). Also highly similar to others e.g. O86835|YA12_STRCO|SC9A10.02 from Streptomyces coelicolor (459 aa), FASTA scores: opt: 1394, E(): 1.1e-83, (51.9% identity in 416 aa overlap); Q04805|YMXG_BACSU|YMXG from Bacillus subtilis (409 aa), FASTA scores: opt: 1014, E(): 7.9e-59, (37.55% identity in 410 aa overlap); Q9KA85|BH2405 from Bacillus halodurans (413 aa), FASTA scores: opt: 967, E(): 9.6e-56, (38.6% identity in 417 aa overlap); etc. Contains PS00143 Insulinase family, zinc-binding region signature. Belongs to peptidase family M16, also known as the insulinase family. Cofactor: requires divalent cations for activity. Binds zinc. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P9WHT5 No intermediary metabolism and respiration P0A5S8 P9WHT5 GO:0006508,GO:0008270,GO:0004222 3.4.24.- P9WHT5 NC_000962.3 Mycobrowser_v5 CDS 3212162 3212956 . - 0 Rv2902c rnhB Degrades the ribonucleotide moiety on RNA-DNA hybrid molecules [catalytic activity: endonucleolytic cleavage to 5'- phosphomonoester]. Probable ribonuclease HII protein RnhB (RNase HII) Rv2902c, (MT2970, MTCY274.33c), len: 264 aa. Probable rnhB, ribonuclease HII, equivalent to O33022|RNH2_MYCLE|RNHB|ML1611|MLCB250.40 ribonuclease HII from Mycobacterium leprae (240 aa), FASTA scores: opt: 1242, E(): 6.9e-72, (76.75% identity in 245 aa overlap). Also similar (but longer ~20 aa) to others e.g. Q9HXY9|RNHB|PA3642 ribonuclease HII from Pseudomonas aeruginosa (201 aa), FASTA scores: opt: 572, E(): 3.1e-29, (52.7% identity in 184 aa overlap); Q9PEI7|RNH2_XYLFA|RNHB|XF1041 ribonuclease HII from Xylella fastidiosa (234 aa), FASTA scores: opt: 556, E(): 3.6e-28, (50.25% identity in 185 aa overlap); P10442|RNH2_ECOLI|RNHB|B0183 ribonuclease HII from Escherichia coli strain K-12 (213 aa), FASTA scores: opt: 519, E(): 7.4e-26, (48.65% identity in 183 aa overlap); etc. Belongs to the RNASE HII family. Cofactor: manganese (by similarity). P9WH01 No information pathways Q10793 P9WH01 GO:0005737,GO:0046872,GO:0004523,GO:0003723 3.1.26.4 P9WH01 NC_000962.3 Mycobrowser_v5 CDS 3093905 3094900 . - 0 Rv2786c ribF Involved in FAD biosynthesis [catalytic activity 1: ATP + riboflavin = ADP + FMN] [catalytic activity 2: ATP + FMN = diphosphate + FAD]. Probable bifunctional FAD synthetase/riboflavin biosynthesis protein RibF: riboflavin kinase (flavokinase) + FMN adenylyltransferase (FAD pyrophosphorylase) (FAD synthetase)(FAD diphosphorylase) (flavin adenine dinucleotide synthetase) Rv2786c, (MTV002.51c), len: 331 aa. Probable ribF, FAD synthetase/riboflavin biosynthesis protein, bifunctional enzyme, equivalent to O32968|RIBF|ML0852 riboflavin kinase from Mycobacterium leprae (331 aa), FASTA scores: opt: 1923, E(): 2.3e-115, (87.45% identity in 327 aa overlap). Also highly similar to many e.g. Q59263|RIBF_CORAM from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (338 aa), FASTA scores: opt: 899, E(): 5.7e-50, (45.8% identity in 321 aa overlap); Q9Z530|SC9F2.05c from Streptomyces coelicolor (318 aa), FASTA scores: opt: 862, E(): 1.3e-47, (52.45% identity in 324 aa overlap); P08391|RIBF_ECOLI|B0025|Z0029ECS0028 from Escherichia coli strains K12 and O157:H7 (313 aa), FASTA scores: opt: 517, E(): 1.3e-25, (36.05% identity in 305 aa overlap); etc. I6X5C9 No intermediary metabolism and respiration O33328 I6X5C9 GO:0009231,GO:0008531,GO:0003919 2.7.1.26 I6X5C9 NC_000962.3 Mycobrowser_v5 CDS 3098964 3100169 . - 0 Rv2790c ltp1 Possibly catalyzes the transfer of a great variety of lipids between membranes. Probable lipid-transfer protein Ltp1 Rv2790c, (MTV002.55c), len: 401 aa. Probable ltp1, lipid-transfer protein, highly similar to many eukaryotic sterol-carrier proteins/lipid-transfer protein precursors (see Ossendorp & Wirtz 1993) e.g. O62742|SCP2 sterol carrier protein X from Oryctolagus cuniculus (Rabbit) (547 aa), FASTA scores: opt: 1710, E(): 6e-102, (63.7% identity in 394 aa overlap); Q9QW19 3-oxoacyl-CoA thiolase homolog (fragment) from Rattus sp. (405 aa), FASTA scores: opt: 1696, E(): 3.8e-101, (63.2% identity in 394 aa overlap); P11915|NLTP_RAT|SCP2|SCP-2 nonspecific lipid-transfer protein precursor from Rattus norvegicus (Rat) (547 aa), FASTA scores: opt: 1696, E(): 4.8e-101, (63.2% identity in 394 aa overlap); P32020|NLTP_MOUSE|SCP2|SCP-2 nonspecific lipid-transfer protein precursor from Mus musculus (Mouse) (547 aa), FASTA scores: opt: 1681, E(): 4.3e-100, (62.7% identity in 394 aa overlap); etc. Contains PS00098 Thiolases acyl-enzyme intermediate signature and PS00737 Thiolases signature 2. Also similar to other M. tuberculosis proteins e.g. O06144|Rv1627c|MTCY01B2.19c (402 aa) (35.8% identity in 413 aa overlap). O33332 No cell wall and cell processes O33332 O33332 GO:0016747,GO:0008152 O33332 NC_000962.3 Mycobrowser_v5 CDS 3113658 3114812 . + 0 Rv2807 Rv2807 Function unknown Conserved hypothetical protein Rv2807, (MTCY16B7.36c), len: 384 aa. Conserved hypothetical protein, highly similar, but shorter 35 aa, to Q9KK74 hypothetical 47.4 KDA protein from Brevibacterium linens (418 aa), FASTA scores: opt: 1865, E(): 9.4e-116, (69.75% identity in 380 aa overlap); and with similarity with other hypothetical proteins or transposases e.g. Q981U5|MLR9230 protein from Rhizobium loti (Mesorhizobium loti) (504 aa), FASTA scores: opt: 636,, (36.05% identity in 377 aa overlap); CAC47689 putative transposase for insertion sequence ISRM18 from Rhizobium meliloti (Sinorhizobium meliloti) (507 aa), FASTA scores: opt: 553, E(): 6.6e-29, (33.5% identity in 370 aa overlap); etc. Also similar to Rv3128c|MTCY164.38c (336 aa) (47.2% identity in 339 aa overlap); and high similarity at N-terminal region with Rv2805|MTCY16B7.38c (79.2% identity in 101 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P71644 No conserved hypotheticals P71644 P71644 GO:0015074,GO:0003677 P71644 NC_000962.3 Mycobrowser_v5 CDS 3118224 3119036 . + 0 Rv2813 Rv2813 Function unknown Conserved hypothetical protein Rv2813, (MTCY16B7.30c), len: 270 aa. Conserved hypothetical protein, similar to various proteins (notably secreted proteins) e.g. Q9ZFL2 hypothetical 30.4 KDA protein from Bacillus stearothermophilus (266 aa), FASTA scores: opt: 518, E(): 1.4e-26, (33.85% identity in 266 aa overlap); P45754|GSPA_AERHY|EXEA general secretion pathway protein from Aeromonas hydrophila (547 aa), FASTA scores: opt: 386, E(): 1.1e-17, (32.05% identity in 265 aa overlap); Q9KPC7|VC2445 general secretion pathway protein A from Vibrio cholerae (529 aa), FASTA scores: opt: 366, E(): 2.2e-16, (31.1% identity in 270 aa overlap); Q56674|VC0403 mannose-sensitive hemagglutinin D from Vibrio cholerae (281 aa), FASTA scores: opt: 317, E(): 2.1e-13, (27.85% identity in 262 aa overlap); etc. Also highly similar to AAK40072 Rv2813-like protein from Mycobacterium celatum (270 aa), FASTA scores: opt: 1628, E(): 2.8e-99, (90.75% identity in 270 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6XFD1 No conserved hypotheticals P71638 I6XFD1 I6XFD1 NC_000962.3 Mycobrowser_v5 CDS 3120566 3121552 . - 0 Rv2814c Rv2814c Required for the transposition of the insertion element IS6110. Probable transposase Rv2814c, (MTCY16B7.29), len: 328 aa. Probable transposase subunit for IS6110. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2814c and Rv2815c, the sequence UUUUAAAG (directly upstream of Rv2814c) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 16 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WKH9 No insertion seqs and phages P0C5G8 P9WKH9 GO:0015074,GO:0006310,GO:0032196,GO:0003677 P9WKH9 NC_000962.3 Mycobrowser_v5 CDS 3397214 3398290 . - 0 Rv3037c Rv3037c Function unknown Conserved hypothetical protein Rv3037c, (MTV012.52c), len: 358 aa. Conserved hypothetical protein, similar in part to others e.g. O86799|SC6G4.36c from Streptomyces coelicolor (426 aa), FASTA scores: opt: 545, E(): 5.5e-27, (36.15% identity in 354 aa overlap); Q9UZW6|PAB0687 from Pyrococcus abyssi (386 aa), FASTA scores: opt: 262, E(): 3.5e-09, (31.0% identity in 200 aa overlap). P9WJZ3 No conserved hypotheticals P9WJZ3 P9WJZ3 NC_000962.3 Mycobrowser_v5 CDS 3128253 3128963 . - 0 Rv2821c Rv2821c Function unknown Conserved hypothetical protein Rv2821c, (MTCY16B7.21), len: 236 aa. Conserved hypothetical protein, similar to several hypothetical proteins e.g. Q9X2C9|TM1809 conserved hypothetical protein from Thermotoga maritima (247 aa), FASTA scores: opt: 318, E(): 8.2e-15, (39.45% identity in 213 aa overlap); O27152|MTH1080 conserved hypothetical protein from Methanothermobacter thermautotrophicus (245 aa), FASTA scores: opt: 294, E(): 3.9e-13, (34.8% identity in 224 aa overlap); BAB59251|TVG0114661 hypothetical protein from Thermoplasma volcanium (229 aa), FASTA scores: opt: 252, E(): 3.3e-10, (33.8% identity in 225 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WJF9 No conserved hypotheticals P71631 P9WJF9 P9WJF9 NC_000962.3 Mycobrowser_v5 CDS 3137271 3138020 . + 0 Rv2831 echA16 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Probable enoyl-CoA hydratase EchA16 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv2831, (MTCY16B7.11c), len: 249 aa. Probable echA16, enoyl-CoA hydratase, similar to others e.g. O23468|AT4G16210 from Arabidopsis thaliana (Mouse-ear cress) (244 aa), FASTA scores: opt: 491, E(): 7.3e-25, (42.1% identity in 190 aa overlap); Q98LI4|MLL1009 from Rhizobium loti (Mesorhizobium loti) (258 aa), FASTA scores: opt: 491, E(): 7.6e-25, (40.75% identity in 248 aa overlap); O07137|ECH8_MYCLE|ML2402|MLCB1306.05c from Mycobacterium leprae (257 aa), FASTA scores: opt: 478, E(): 5.3e-24, (38.05% identity in 226 aa overlap); P76082|PAAF_ECOLI|B1393 from scherichia coli strain K12 (255 aa), FASTA scores: opt: 439, E(): 1.9e-21, (37.55% identity in 221 aa overlap); etc. Also similar to O53418|ECH8_MYCTU|ECHA8|Rv1070c|MT1100|MTV017.23c from Mycobacterium tuberculosis (257 aa), FASTA scores: opt: 471, E(): 1.5e-23, (38.05% identity in 226 aa overlap). I6YEH6 No lipid metabolism P71621 I6YEH6 GO:0016853,GO:0008152,GO:0004300 4.2.1.17 I6YEH6 NC_000962.3 Mycobrowser_v5 CDS 3138099 3139181 . - 0 Rv2832c ugpC Thought to be involved in active transport of Sn-glycerol-3-phosphate across the membrane (import). Responsible for energy coupling to the transport system. Probable Sn-glycerol-3-phosphate transport ATP-binding protein ABC transporter UgpC Rv2832c, (MTCY16B7.10), len: 360 aa. Probable ugpC, Sn-glycerol-3-phosphate transport ATP-binding protein ABC transporter (see Braibant et al., 2000), similar to others: CAC48805 probable glycerol-3-phosphate ABC transporter ATP-binding protein from Rhizobium meliloti (Sinorhizobium meliloti) plasmid pSymB (349 aa), FASTA scores: opt: 1018, E(): 4.1e-53, (48.6% identity in 356 aa overlap); Q98G42|MLL3499|UGPC SN-glycerol-3-phosphate transport ATP-binding protein from Rhizobium loti (Mesorhizobium loti) (366 aa), FASTA scores: opt: 1016, E(): 5.6e-53, (48.5% identity in 367 aa overlap). But also highly similar to many msiK proteins, ABC transporter ATP-binding proteins possibly involved in transport of cellolbiose and maltose (see Schlosser et al., 1997) e.g. P96483|MSIK MSIK protein from Streptomyces reticuli (377 aa), FASTA scores: opt: 1277, E(): 1.9e-68, (58.05% identity in 379 aa overlap); Q9L0Q1|MSIK ABC transporter ATP-binding protein from Streptomyces coelicolor (378 aa), FASTA scores: opt: 1276, E(): 2.1e-68, (57.65% identity in 380 aa overlap); Q54333|MSIK from Streptomyces lividans (314 aa), FASTA scores: opt: 1217, E(): 5.9e-65, (63.7% identity in 292 aa overlap); and other ABC-type sugar transport proteins. Also highly similar to O53482|Rv2038c|MTV018.25c ABC-type sugar transport protein from Mycobacterium tuberculosis (357 aa), FASTA scores: opt: 1248, E(): 9.4e-67, (56.8% identity in 354 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). I6X5H3 No cell wall and cell processes P71620 I6X5H3 GO:0043190,GO:0016887,GO:0016820,GO:0006810,GO:0005524 I6X5H3 NC_000962.3 Mycobrowser_v5 CDS 3140487 3141314 . - 0 Rv2834c ugpE Involved in active transport of Sn-glycerol-3-phosphate across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable Sn-glycerol-3-phosphate transport integral membrane protein ABC transporter UgpE Rv2834c, (MTCY16B7.08), len: 275 aa. Probable ugpE, Sn-glycerol-3-phosphate transport integral membrane protein ABC transporter (see citation below), similar to various permeases e.g. Q9KDY3|BH1078 glycerol-3-phosphate ABC transporter from Bacillus halodurans (270 aa), FASTA scores: opt: 620, E(): 4.3e-32, (34.7% identity in 268 aa overlap); Q9X0K6|TM1122 glycerol-3-phosphate ABC transporter permease protein from Thermotoga maritima (276 aa), FASTA scores: opt: 605, E(): 3.9e-31, (32.5% identity in 274 aa overlap); AAG58557|UGPE SN-glycerol 3-phosphate transport system (integral membrane protein) from Escherichia coli strain O157:H7 and EDL933 (281 aa), FASTA scores: opt: 574, E(): 3.7e-29, (32.95% identity in 264 aa overlap); P10906|UGPE_ECOLI|B3451 SN-glycerol-3-phosphate transport system permease protein from Escherichia coli strain K12 (281 aa), FASTA scores: opt: 569, E(): 7.6e-29, (32.6% identity in 264 aa overlap); etc. Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature. I6Y1U3 No cell wall and cell processes P71618 I6Y1U3 GO:0005886,GO:0006810,GO:0005215,GO:0016021 I6Y1U3 NC_000962.3 Mycobrowser_v5 CDS 3141311 3142222 . - 0 Rv2835c ugpA Involved in active transport of Sn-glycerol-3-phosphate across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable Sn-glycerol-3-phosphate transport integral membrane protein ABC transporter UgpA Rv2835c, (MTCY1B7.07), len: 303 aa. Probable ugpA, Sn-glycerol-3-phosphate transport integral membrane protein ABC transporter (see citation below), similar to various permeases e.g. Q9RK71|SCF11.19 probable sugar transporter inner membrane protein from Streptomyces coelicolor (316 aa), FASTA scores: opt: 643, E(): 3.1e-35, (38.85% identity in 291 aa overlap); Q9KDY4|BH1077 glycerol-3-phosphate ABC transporter (permease) from Bacillus halodurans (315 aa), FASTA scores: opt: 548, E(): 6.2e-29, (31.5% identity in 295 aa overlap); AAK78407|CAC0427 glycerol-3-phosphate ABC-transporter, permease component from Clostridium acetobutylicum (304 aa), FASTA scores: opt: 538, E(): 2.8e-28, (29.1% identity in 292 aa overlap); etc. Contains PS00062 Aldo/keto reductase family signature 2, and PS00402 Binding-protein-dependent transport systems inner membrane comp signature. I6XFF3 No cell wall and cell processes P71617 I6XFF3 GO:0005886,GO:0006810,GO:0005215,GO:0016021 I6XFF3 NC_000962.3 Mycobrowser_v5 CDS 3728624 3729355 . + 0 Rv3342 Rv3342 Causes methylation Possible methyltransferase (methylase) Rv3342, (MTV016.42), len: 243 aa. Possible methyltransferase, similar to various proteins e.g. Q9I5X8|PA0558 hypothetical protein from Pseudomonas aeruginosa (255 aa), FASTA scores: opt: 496, E(): 4.4e-24, (39.85% identity in 236 aa overlap); Q9XBC9|CZA382.22c putative rRNA methylase from Amycolatopsis orientalis (259 aa), FASTA scores: opt: 473, E(): 1.2e-22, (42.45% identity in 245 aa overlap); Q9UTA8|SPAC25B8.10 putative methyltransferase from Schizosaccharomyces pombe (Fission yeast) (256 aa), FASTA scores: opt: 470, E(): 1.9e-22, (35.7% identity in 238 aa overlap); and Q9UTA9|SPAC25B8.09 putative methyltransferase from Schizosaccharomyces pombe (Fission yeast) (251 aa), FASTA scores: opt: 418, E(): 3.4e-19, (31.2% identity in 237 aa overlap); etc. Start uncertain. Belongs to the methyltransferase superfamily. P9WK01 No intermediary metabolism and respiration P65348 P9WK01 GO:0008168,GO:0008152 2.1.1.- P9WK01 NC_000962.3 Mycobrowser_v5 CDS 3148385 3149428 . - 0 Rv2841c nusA Could participates in both the termination and antitermination of transcription. Probable N utilization substance protein A NusA Rv2841c, (MTCY24A1.16, MTCY16B7.01), len: 347 aa. Probable nusA, N-utilization substance protein A, equivalent to Q9Z5J1|NUSA|ML1558 probable transcription termination/antitermination factor from Mycobacterium leprae (347 aa), FASTA scores: opt: 2054, E(): 5.4e-120, (91.95% identity in 347 aa overlap). Also highly similar to others e.g. Q9KYR1|SC5H4.28 putative transcriptional termination/antitermination factor from Streptomyces coelicolor (340 aa), FASTA scores: opt: 1346, E(): 4.3e-76, (63.35% identity in 341 aa overlap); P32727|NUSA_BACSU N utilization substance protein A (371 aa), FASTA scores: opt: 847, E(): 4.1e-45, (43.95% identity in 346 aa overlap); Q9KA74|NUSA|BH2416 transcriptional terminator from Bacillus halodurans (382 aa), FASTA scores: opt: 846, E(): 4.8e-45, (43.15% identity in 373 aa overlap); etc. Belongs to the NUSA family. P9WIV3 No information pathways 1K0R,2ASB,2ATW P0A5M2 P9WIV3 GO:0005515,GO:0031564,GO:0003700,GO:0006353,GO:0003723 P9WIV3 NC_000962.3 Mycobrowser_v5 CDS 3151202 3152950 . - 0 Rv2845c proS Involved in translation mechanism [catalytic activity: ATP + L-proline + tRNA(pro) = AMP + pyrophosphate + L-prolyl-tRNA(pro)]. Probable prolyl-tRNA synthetase ProS (proline--tRNA ligase) (PRORS) (global RNA synthesis factor) (proline translase) Rv2845c, (MTCY24A1.12), len: 582 aa. Probable proS, prolyl-tRNA synthetase, highly similar to others e.g. Q9KYR6|SYP_STRCO|pros|SC5H4.23 from Streptomyces coelicolor (567 aa), FASTA scores: opt: 1161, E(): 9e-64, (57.15% identity in 574 aa overlap); P56124|SYP_HELPY|pros|HP0238 from Helicobacter pylori (Campylobacter pylori) (577 aa), FASTA scores: opt: 1082, E(): 6.6e-59, (37.8% identity in 553 aa overlap); P16659|SYP_ECOLI|pros|DRPA|B0194 from Escherichia coli strain K12 (572 aa), FASTA scores: opt: 926, E(): 2.6e-49, (39.85% identity in 587 aa overlap); etc. Contains PS00179 Aminoacyl-transfer RNA synthetases class-II signature 1. Belongs to class-II aminoacyl-tRNA synthetase family. P9WFT9 No information pathways O05814 P9WFT9 GO:0005737,GO:0004827,GO:0006433,GO:0005524 6.1.1.15 P9WFT9 NC_000962.3 Mycobrowser_v5 CDS 3156148 3157521 . - 0 Rv2848c cobB Involved in the cobalamin biosynthesis. Responsible for the amidation of carboxylic groups at position a and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation. Probable cobyrinic acid A,C-diamide synthase CobB Rv2848c, (MTCY24A1.09), len: 457 aa. Probable cobB, cobyrinic acid A,C-diamide synthase, highly similar to others e.g. O27509|COBB_METTH|MTH1460 from Methanobacterium thermoautotrophicum (447 aa), FASTA scores: opt: 980, E(): 1.3e-49, (39.65% identity in 454 aa overlap); Q9KBM8|BH1898 from Bacillus halodurans (465 aa), FASTA scores: opt: 928, E(): 1.4e-46, (37.0% identity in 457 aa overlap); O68108|COBB_RHOCA from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (435 aa), FASTA scores: opt: 921, E(): 3.3e-46, (39.35% identity in 437 aa overlap); etc. Belongs to the COBB/COBQ family, COBB subfamily. P9WP97 No intermediary metabolism and respiration P63835 P9WP97 GO:0015420,GO:0042242,GO:0006541,GO:0009236 P9WP97 NC_000962.3 Mycobrowser_v5 CDS 3398425 3399408 . - 0 Rv3038c Rv3038c Function unknown Conserved protein Rv3038c, (MTV012.53c), len: 327 aa. Conserved protein, equivalent to Q9CBQ9|ML1723 hypothetical protein from Mycobacterium leprae (327 aa), FASTA scores: opt: 1843, E(): 6.1e-108, (80.75% identity in 327 aa overlap). Weak similarity with e.g. Q9KZI3|SCG8A.16 putative methyltransferase from Streptomyces coelicolor (199 aa), FASTA scores: opt: 227, E(): 3.9e-07, (31.95% identity in 191 aa overlap) and O52570 methyltransferase from Amycolatopsis mediterranei (272 aa), FASTA scores: opt: 228, E(): 4.3e-07, (31.7% identity in 164 aa overlap). Contains PS00044 Bacterial regulatory proteins, lysR family signature but shows no similarity to known LysR family members. I6YAZ1 No conserved hypotheticals O53285 I6YAZ1 GO:0008168,GO:0008152 I6YAZ1 NC_000962.3 Mycobrowser_v5 CDS 3213912 3214253 . - 0 Rv2904c rplS This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site. 50S ribosomal protein L19 RplS Rv2904c, (MTCY274.35c), len: 113 aa. rplS, 50S ribosomal protein L19, equivalent to O33020|RL19_MYCLE 50S ribosomal protein L19 from Mycobacterium leprae (113 aa), FASTA scores: opt: 702, E(): 1.4e-45, (93.8% identity in 113 aa overlap). Also highly similar to others e.g. O69883|RL19_STRCO from Streptomyces coelicolor (116 aa), FASTA scores: opt: 571, E(): 9.5e-36, (77.25% identity in 110 aa overlap); O31742|RL19_BACSU from Bacillus subtilis (115 aa), FASTA scores: opt: 523, E(): 3.8e-32, (72.9% identity in 107 aa overlap); RL19_BACST|P30529 from Bacillus stearothermophilus (116 aa), FASTA scores: opt: 518, E(): 9.1e-32, (71.7% identity in 106 aa overlap); etc. Belongs to the L19P family of ribosomal proteins. P9WHC9 No information pathways P66080 P9WHC9 GO:0003735,GO:0006412,GO:0005840 P9WHC9 NC_000962.3 Mycobrowser_v5 CDS 3160580 3162061 . - 0 Rv2852c mqo Involved in tricarboxylic acid cycle [catalytic activity: (S)-malate + acceptor = oxaloacetate + reduced acceptor]. Probable malate:quinone oxidoreductase Mqo (malate dehydrogenase [acceptor]) Rv2852c, (MT2918, MTCY24A1.05), len: 493 aa. Probable mqo, malate:quinone oxidoreductase, highly similar to others e.g. O69282|MQO_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (499 aa), FASTA scores: opt: 1701, E(): 1.2e-101, (50.7% identity in 495 aa overlap); Q9Z9Q7|BH3960 from Bacillus halodurans (500 aa), FASTA scores: opt: 1632, E(): 3.3e-97, (48.55% identity in 486 aa overlap); Q9HYF4|MQOA|PA3452 from Pseudomonas aeruginosa (523 aa), FASTA scores: opt: 1604, E(): 2.1e-95, (49.1% identity in 487 aa overlap) (N-terminus longer); P33940|MQO_ECOLI|B2210 from Escherichia coli strain K12 (548 aa), FASTA scores: opt: 1525, E(): 2.7e-90, (48.15% identity in 492 aa overlap); etc. Belongs to the MQO family. Cofactors: FAD. P9WJP5 No intermediary metabolism and respiration P65419 P9WJP5 GO:0055114,GO:0006099,GO:0008924 1.1.5.4 NC_000962.3 Mycobrowser_v5 CDS 3165205 3166584 . + 0 Rv2855 mtr Involved in reduction of mycothiol. NADPH-dependent mycothiol reductase Mtr Rv2855, (MTCY24A1.02c), len: 459 aa. Mtr, NADPH-dependent mycothiol reductase, proven enzymatically but previously described as glutathione reductase homolog (gene name: gorA) (see citation below). Similar to others e.g. Q9L7K8|MERA mercuric reductase from Streptomyces sp. CHR28 (474 aa), FASTA scores: opt: 719, E(): 9e-38, (35.2% identity in 460 aa overlap); P30341|MERA_STRLI mercuric reductase from Streptomyces lividans (474 aa), FASTA scores: opt: 712, E(): 2.5e-37, (34.95% identity in 455 aa overlap); Q98ED5|MLL4296 ferric leghemoglobin reductase-2 precursor, dihydrolipoamide dehydrogenase from Rhizobium loti (Mesorhizobium loti) (468 aa), FASTA scores: opt: 670, E(): 1.1e-34, (30.8% identity in 471 aa overlap); etc. Belongs to the pyridine nucleotide-disulphide oxidoreductases class-I. Cofactor: FAD. P9WHH3 No intermediary metabolism and respiration O07927 P9WHH3 GO:0045454,GO:0005737,GO:0055114,GO:0016491,GO:0050660 1.-.-.- P9WHH3 NC_000962.3 Mycobrowser_v5 CDS 3166684 3167802 . + 0 Rv2856 nicT Seems involved in nickel incorporation. Thought to be involved in transport of nickel across the membrane. Responsible for the translocation of the substrate across the membrane. Possible nickel-transport integral membrane protein NicT Rv2856, (MTCY24A1.01c), len: 372 aa. Possible nicT, nickel-transport integral membrane protein, similar to transport proteins and hydrogenase cluster proteins e.g. BAB58860|SAV2698 hypothetical 37.9 KDA protein from Staphylococcus aureus subsp. aureus Mu50 (338 aa), FASTA scores: opt: 1082, E(): 7.1e-60, (48.05% identity in 335 aa overlap); Q97ZB2|HOXN high-affinity nickel-transport protein from Sulfolobus solfataricus (373 aa), FASTA scores: opt: 922, E(): 6.6e-50, (42.2% identity in 372 aa overlap); P23516|HOXN_ALCEU high-affinity nickel transport protein (integral membrane protein) from Alcaligenes eutrophus (Ralstonia eutropha) (351 aa), FASTA scores: opt: 904, E(): 8.3e-49, (41.9% identity in 339 aa overlap); Q45247|HUPN_BRAJA hydrogenase nickel incorporation protein from Bradyrhizobium japonicum (381 aa), FASTA scores: opt: 853, E(): 1.3e-45, (41.65% identity in 329 aa overlap); etc. Seems to belong to the HOXN/HUPN/NIXA family of nickel transporters (NiCoT family). I6YEJ7 No cell wall and cell processes O05804 I6YEJ7 GO:0016021,GO:0046872,GO:0015099,GO:0015675,GO:0055085 NC_000962.3 Mycobrowser_v5 CDS 3169356 3170723 . - 0 Rv2858c aldC Oxidizes a wide variety of aldehydes [catalytic activity: aldehyde + NAD(+) + H(2)O = acid + NADH]. Probable aldehyde dehydrogenase AldC Rv2858c, (MTV003.04c), len: 455 aa. Probable aldC, aldehyde dehydrogenase, similar to many e.g. O88069|SCI35.34c putative aldehyde dehydrogenase from Streptomyces coelicolor (483 aa), FASTA scores: opt: 1872, E(): 6.4e-109, (64.5% identity in 448 aa overlap); Q9FAB1|ALDH|BT-ALDH aldehyde dehydrogenase from Bacillus thermoleovorans (497 aa), FASTA scores: opt: 1157, E(): 2.1e-64, (44.3% identity in 458 aa overlap); O33455|CYMC P-CUMIC aldehyde dehydrogenase from Pseudomonas putida (494 aa), FASTA scores: opt: 1149, E(): 6.5e-64, (43.15% identity in 452 aa overlap); P40047|DHA5_YEAST|ALD5|ALDH5|ALD3|YER073W aldehyde dehydrogenase from Saccharomyces cerevisiae (Baker's yeast) (519 aa), FASTA scores: opt: 1091, E(): 2.7e-60, (38.55% identity in 459 aa overlap); P80668|FEAB_ECOLI|PADA|MAOB|B1385 phenylacetaldehyde dehydrogenase from Escherichia coli strain K12 (499 aa), FASTA scores: opt: 1074, E(): 3e-59, (42.2% identity in 462 aa overlap); etc. Also similar to many M. tuberculosis dehydrogenases e.g. P71823|Rv0768|MTCY369.13 (489 aa), FASTA score: (38.1% identity in 467 aa overlap). Contains PS00687 Aldehyde dehydrogenases glutamic acid active site and PS00070 Aldehyde dehydrogenases cysteine active site. Belongs to the aldehyde dehydrogenases family. O33340 No intermediary metabolism and respiration O33340 O33340 GO:0055114,GO:0004029 1.2.1.3 O33340 NC_000962.3 Mycobrowser_v5 CDS 3170720 3171646 . - 0 Rv2859c Rv2859c Function unknown; probably involved in cellular metabolism. Possible amidotransferase Rv2859c, (MTV003.05c), len: 308 aa. Possible amidotransferase, equivalent (but longer 58 aa) to Q9CBU9|ML1573 possible amidotransferase from Mycobacterium leprae (249 aa), FASTA scores: opt: 1226, E(): 3e-64, (71.55% identity in 239 aa overlap). Also similar to other amidotransferases and hypothetical proteins, but shorter in N-terminus e.g. O88072|SCI35.37 hypothetical 25.3 KDA protein from Streptomyces coelicolor (242 aa), FASTA scores: opt: 683, E(): 1.2e-32, (47.65% identity in 235 aa overlap); AAK79730|Q97I88|CAC1764 predicted glutamine amidotransferase from Clostridium acetobutylicum (241 aa), FASTA scores: opt: 458, E(): 1.6e-19, (32.95% identity in 246 aa overlap); AAK75201|Q97QV9|SP1089 glutamine amidotransferase class I from Streptococcus pneumoniae (229 aa), FASTA scores: opt: 431, E(): 5.6e-18, (34.75% identity in 236 aa overlap); etc. Contains three 17 aa repeats at the N-terminus very similar to those in other Mycobacterium tuberculosis proteins e.g. Q10699|YY30_MYCTU|Rv2090|MT2151|MTCY49.30 putative 5'-3' exonuclease RV2090. O33341 No intermediary metabolism and respiration O33341 O33341 GO:0016787,GO:0016874,GO:0016740,GO:0006541 6.3.5.-,2.-.-.- O33341 NC_000962.3 Mycobrowser_v5 CDS 3175454 3177265 . - 0 Rv2864c Rv2864c Function unknown, possibly involved in cell wall biosynthesis. Possible penicillin-binding lipoprotein Rv2864c, (MTV003.10c), len: 603 aa. Possible penicillin-binding lipoprotein, probably located in periplasm, equivalent to Q9CBU6|ML1577 probable penicillin binding protein from Mycobacterium leprae (608 aa), FASTA scores: opt: 3352, E(): 2.1e-193, (81.5% identity in 606 aa overlap). Also shows some similarity to others e.g. P72405|PCBR from Streptomyces clavuligerus (551 aa), FASTA scores: opt: 543, E(): 6.1e-25, (28.4% identity in 567 aa overlap); Q9F2L0|SCH63.18c from Streptomyces coelicolor (546 aa), FASTA scores: opt: 519, E(): 1.7e-23, (29.3% identity in 577 aa overlap); Q9RKD1|SCE87.07 from Streptomyces coelicolor (541 aa), FASTA scores: opt: 472, E(): 1.1e-20, (34.3% identity in 318 aa overlap); etc. Equivalent to AAK47258 from Mycobacterium tuberculosis strain CDC1551 (618 aa) but shorter 15 aa. Contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site, and PS00017 ATP/GTP-binding site motif A (P-loop). O33346 No cell wall and cell processes O33346 O33346 GO:0009273,GO:0046677,GO:0008658 O33346 NC_000962.3 Mycobrowser_v5 CDS 3180548 3181762 . - 0 Rv2869c rip Controls membrane composition Membrane bound metalloprotease Rv2869c, (MTV003.15c), len: 404 aa. Rip, metalloprotease, regulates intramembrane proteolysis and controls membrane composition (rip, see Makinoshima and Glickman, 2005). Similar to site two protease (S2P) in higher eukaryotes. Conserved transmembrane protein, equivalent to Q9CBU4|ML1582 probable integral membrane protein from Mycobacterium leprae (404 aa), FASTA scores: opt: 2250, E(): 1.1e-128, (82.2% identity in 404 aa overlap). Also weakly similar to other membrane proteins or hypothetical proteins e.g. Q9A710|CC1916 putative membrane-associated zinc metalloprotease from Caulobacter crescentus (398 aa), FASTA scores: opt: 368, E(): 7.8e-15, (28.1% identity in 427 aa overlap). Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). Cleaves PbpB|Rv2163c in a Zn2+ -dependent manner (See Mukherjee et al., 2009). Cleaves proteins RskA|Rv0444c, RslA|Rv0736, and Rv3912, in M. tuberculosis Erdman (See Sklar et al., 2010). P9WHS3 No cell wall and cell processes O33351 P9WHS3 GO:0046872,GO:0004222,GO:0005886,GO:0005515,GO:0006508,GO:0016021 3.4.24.- P9WHS3 NC_000962.3 Mycobrowser_v5 CDS 3184847 3186934 . + 0 Rv2874 dipZ Could be involved in cytochrome-C biogenesis. Possible integral membrane C-type cytochrome biogenesis protein DipZ Rv2874, (MT2942, MTCY274.05), len: 695 aa. Possible dipZ, cytochrome c-type biogenesis protein (see citation below), probable integral membrane protein, similar in part to others or hypothetical proteins e.g. CAC48606|SMB20213 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (627 aa), FASTA scores: opt: 844, E(): 7.3e-43, (32.65% identity in 643 aa overlap); Q9ZMH0|CCDA or JHP0250 putative cytochrome C-type biogenesis protein from Helicobacter pylori J99 (Campylobacter pylori J99) (239 aa), FASTA scores: opt: 250, E(): 1.4e-07, (27.3% identity in 227 aa overlap); Q9LA04|CCDA C-type cytochrome biogenesis protein from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (252 aa), FASTA scores: opt: 245, E(): 2.9e-07, (27.85% identity in 244 aa overlap); etc. Also similar to O06393|CCSA|Rv0527|MTCY25D10.06 cytochrome C-type biogenesis protein from Mycobacterium tuberculosis (259 aa), FASTA scores: opt: 280, E(): 2.4e-09, (29.3% identity in 239 aa overlap). P9WG63 No intermediary metabolism and respiration 2HYX Q10801 P9WG63 GO:0045454,GO:0017004,GO:0016021,GO:0055114,GO:0016491,GO:0005886,GO:0016209 P9WG63 NC_000962.3 Mycobrowser_v5 CDS 3188008 3188871 . - 0 Rv2877c Rv2877c Unknown; possibly involved in transport of mercury across the membrane. Probable conserved integral membrane protein Rv2877c, (MTCY274.08c), len: 287 aa. Probable conserved integral membrane protein, Mer family possibly involved in transport of mercury, similar to others, and to the fourth protein of the mercury resistance operon of Streptomyces sp (or other organisms), and to putative cytochrome-c biogenesis proteins e.g. Q9XBD1|CZA382.20C putative integral membrane transporter from Amycolatopsis orientalis (298 aa), FASTA scores: opt: 913, E(): 7.6e-46, (51.55% identity in 293 aa overlap); P30344|MER4_STRLI mercury resistance probable HG transport protein from Streptomyces lividans (319 aa), FASTA scores: opt: 427, E(): 1.2e-17, (32.85% identity in 289 aa overlap); Q9M5P3 putative cytochrome C biogenesis protein precursor from Arabidopsis thaliana (Mouse-ear cress) (354 aa), FASTA scores: opt: 229, E(): 4e-06, (29.85% identity in 221 aa overlap); etc. Contains PS00044 Bacterial regulatory proteins, lysR family signature. Note that previously known as merT. I6YEL8 No cell wall and cell processes Q7D6F5 I6YEL8 GO:0016020,GO:0055114,GO:0017004 NC_000962.3 Mycobrowser_v5 CDS 3383885 3385066 . - 0 Rv3025c iscS Catalyzes the removal of elemental sulfur from cysteine to produce alanine. Cysteine desulfurase IscS (NIFS protein homolog) (nitrogenase metalloclusters biosynthesis protein NIFS) Rv3025c, (MTV012.40c), len: 393 aa. IscS (alternate gene name: nifS), cysteine desulfurase (NifS-like protein) , equivalent to MLCB637.06|O33098 NIFS-like protein from Mycobacterium leprae (396 aa), FASTA scores: opt: 2186, E(): 2.7e-122, (84.9% identity in 391 aa overlap). Also highly similar to many e.g. O86581|SC2A11.20 putative pyridoxal-phosphate-dependent aminotransferase from Streptomyces coelicolor (389 aa), FASTA scores: opt: 1568, E(): 1.1e-85, (61.7% identity in 389 aa overlap); P57795|ISCS|NIFS cysteine desulfurase (NIFS protein homolog) from Methanosarcina thermophila (404 aa), FASTA scores: opt: 1059, E(): 1.6e-55, (46.2% identity in 381 aa overlap); O54055|ISCS_RUMFL|ISCS|NIFS cysteine desulfurase from Ruminococcus flavefaciens (396 aa), FASTA scores: opt: 973, E(): 2e-50, (43.3% identity in 381 aa overlap); P57794|NIFS_ACEDI cysteine desulfurase from Acetobacter diazotrophicus (400 aa), FASTA scores: opt: 958, E(): 1.6e-49, (41.1% identity in 392 aa overlap); etc. Also similar to Rv1464|MTV007.11 from Mycobacterium tuberculosis. Contains PS00595 Aminotransferases class-V pyridoxal-phosphate attachment site. Belongs to class-V of pyridoxal-phosphate-dependent aminotransferases, NIFS/ISCS subfamily. Cofactor: pyridoxal phosphate (by similarity). P9WQ71 No intermediary metabolism and respiration O53272 P9WQ71 GO:0008152,GO:0030170,GO:0008483,GO:0016829 4.4.1.- P9WQ71 NC_000962.3 Mycobrowser_v5 CDS 3189583 3190152 . - 0 Rv2879c Rv2879c Function unknown Conserved hypothetical protein Rv2879c, (MTCY274.10c), len: 189 aa. Conserved hypothetical protein, similar to others e.g. C-terminus of Q9RVT6|DR0936 conserved hypothetical protein from Deinococcus radiodurans (346 aa), FASTA scores: opt: 505, E(): 1e-26, (46.5% identity in 185 aa overlap); O34617|YLON_BACSU hypothetical 41.6 KDA protein from Bacillus subtilis (363 aa), FASTA scores: opt: 459, E(): 1.2e-24, (40.5% identity in 185 aa overlap); YFGB_ECOLI|P36979 hypothetical 43.1 kDa protein from Escherichia coli (384 aa), FASTA scores, opt: 410, E(): 2.8e-21, (41.7% identity in 187 aa overlap); etc. Appears to be a frame shift with respect to following ORF but we can detect no error in the cosmid sequence to account for this. P0A644 No conserved hypotheticals P0A644 P0A644 GO:0008173,GO:0008757,GO:0005737,GO:0046872,GO:0006364,GO:0051539 2.1.1.- P9WH15 NC_000962.3 Mycobrowser_v5 CDS 3190701 3191621 . - 0 Rv2881c cdsA Involved in the phospholipid biosynthesis [catalytic activity: CTP + phosphatidate = pyrophosphate + CDP-diacylglycerol]. Probable integral membrane phosphatidate cytidylyltransferase CdsA (CDP-diglyceride synthetase) (CDP-diglyceride pyrophosphorylase) (CDP-diacylglycerol synthase) (CDS) (CTP:phosphatidate cytidylyltransferase) (CDP-DAG synthase) (CDP-DG synthetase) Rv2881c, (MTCY274.12c), len: 306 aa. Probable cdsA, phosphatidate cytidylyltransferase, integral membrane protein, equivalent to Q9CBU1|CDSA_MYCLE|ML1589 phosphatidate cytidylyltransferase from Mycobacterium leprae (312 aa), FASTA scores: opt: 1470, E(): 1.1e-84, (70.3% identity in 313 aa overlap). Also similar to others e.g. Q9KPV7|VC2255 from Vibrio cholerae (280 aa), FASTA scores: opt: 383, E(): 1.1e-16, (29.3% identity in 280 aa overlap); Q9CDT2|CDSA from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (267 aa), FASTA scores: opt: 361, E(): 2.6e-15, (29.05% identity in 265 aa overlap); P06466|CDSA_ECOLI|CDS|B0175|Z0186|ECS0177 from Escherichia coli strains K12 and O157:H7 (249 aa), FASTA scores: opt: 352, E(): 9.2e-15, (40.4% identity in 156 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the CDS family. P9WPF7 No lipid metabolism P63758 P9WPF7 GO:0004605,GO:0008654,GO:0005886,GO:0016021 2.7.7.41 P9WPF7 NC_000962.3 Mycobrowser_v5 CDS 3193393 3194151 . + 0 Rv2884 Rv2884 Involved in transcriptional mechanism Probable transcriptional regulatory protein Rv2884, (MTCY274.15), len: 252 aa. Probable transcriptional regulatory protein, highly similar to others e.g. Q05943|GLNR_STRCO|SCD84.26c transcriptional regulatory protein from Streptomyces coelicolor (267 aa), FASTA scores: opt: 609, E(): 2.7e-34, (46.4% identity in 224 aa overlap); Q55733|SLL0396 regulatory components of sensory transduction system from Synechocystis sp. strain PCC 6803 (224 aa), FASTA scores: opt: 330, E(): 3e-15, (31.8% identity in 217 aa overlap); Q9A4S3|CC2757 DNA-binding response regulator from Caulobacter crescentus (223 aa), FASTA scores: opt: 311, E(): 6e-14, (30.3% identity in 221 aa overlap); etc. Also highly similar to O53830|Rv0818|MTV043.10 putative regulatory protein from Mycobacterium tuberculosis (255 aa), FASTA scores: opt: 665, E(): 3.8e-38, (47.6% identity in 227 aa overlap). The N-terminal region is similar to that of other regulatory components of sensory transduction systems. I6X5M3 No regulatory proteins Q10808 I6X5M3 GO:0005622,GO:0006355,GO:0000156,GO:0000160,GO:0003677 I6X5M3 NC_000962.3 Mycobrowser_v5 CDS 3198292 3199107 . - 0 Rv2889c tsf Associates with the EF-TU.GDP complex and induces the exchange of GDP to GTP, it remains bound to the aminoacyl-tRNA. EF-TU.GTP complex up to the GTP hydrolysis stage on the ribosome. Probable elongation factor Tsf (EF-ts) Rv2889c, (MTCY274.20c), len: 271 aa. Probable tsf, elongation factor, equivalent to O33039|EFTS_MYCLE|TSF|ML1597|MLCB250.64 elongation factor from Mycobacterium leprae (276 aa), FASTA scores: opt: 1430, E(): 1.9e-80, (83.7% identity in 276 aa overlap). Also highly similar to others e.g. Q9X5Z9|EFTS_STRRA|TSF from Streptomyces ramocissimus (278 aa), FASTA scores: opt: 928, E(): 1.1e-49, (57.05% identity in 277 aa overlap); O31213|EFTS_STRCO|TSF|SC2E1.42 from Streptomyces coelicolor (278 aa), FASTA scores: opt: 927, E(): 1.3e-49, (56.3% identity in 277 aa overlap); P80700|EFTS_BACSU|TSF from Bacillus subtilis (292 aa), FASTA scores: opt: 650, E(): 1.3e-32, (43.85% identity in 276 aa overlap); etc. Contains PS01127 Elongation factor Ts signature 2. Belongs to the EF-ts family. P9WNM1 No information pathways Q10788 P9WNM1 GO:0003746,GO:0006414,GO:0005737 P9WNM1 NC_000962.3 Mycobrowser_v5 CDS 3202420 3203397 . + 0 Rv2893 Rv2893 Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv2893, (MTCY274.24), len: 325 aa. Possible oxidoreductase, showing similarity with various proteins and/or oxidoreductases e.g. Q9AE05|RIF11 eleventh protein in the rif biosynthetic gene cluster from Amycolatopsis mediterranei (Nocardia mediterranei) (294 aa), FASTA scores: opt: 270, E(): 4.8e-10, (34.5% identity in 313 aa overlap); O52567 reductase from Amycolatopsis mediterranei (Nocardia mediterranei) (153 aa), FASTA scores: opt: 251, E(): 5e-09, (42.4% identity in 125 aa overlap); Q58929|mer|MJ1534 F420-dependent methylenetetrahydromethanopterin reductase from Methanococcus jannaschii (331 aa), FASTA scores: opt: 249, E(): 1.2e-08, (29.7% identity in 283 aa overlap); etc. Also some similarity with others proteins from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. P71844|Rv0791c|MTCY369.35c putative oxidoreductase (347 aa), FASTA scores: opt: 264, E(): 1.3e-09, (29.05% identity in 272 aa overlap); and P96809|Rv0132|MTCI5.06c putative oxidoreductase (360 aa), FASTA scores: opt: 260, E(): 2.4e-09, (33.05% identity in 239 aa overlap). I6YEN3 No intermediary metabolism and respiration Q10814 I6YEN3 GO:0016491,GO:0055114 1.-.-.- I6YEN3 NC_000962.3 Mycobrowser_v5 CDS 3206431 3207942 . - 0 Rv2897c Rv2897c Function unknown Conserved hypothetical protein Rv2897c, (MTCY274.28c), len: 503 aa. Conserved hypothetical protein, possibly Mg-chelatase, highly similar to hypothetical proteins and chelatases e.g. Q9RTV0|DR1656 mg(2+) chelatase family protein from Deinococcus radiodurans (519 aa), FASTA scores: opt: 1333, E(): 3.6e-68, (46.55% identity in 505 aa overlap);Q55372|SLR0904 hypothetical 55.1 KDA protein from Synechocystis sp. strain PCC 6803 (509 aa), FASTA scores: opt: 1271, E(): 1.2e-64, (42.65% identity in 504 aa overlap); Q9HTR4|PA5290 hypothetical protein from Pseudomonas aeruginosa (497 aa), FASTA scores: opt: 1248, E(): 2.3e-63, (45.9% identity in 503 aa overlap); Q9K0Z6|comm|NMB0405 competence protein (mg-chelatase) from Neisseria meningitidis (serogroup B), FASTA scores: opt: 1229, E(): 2.8e-62, (43.2% identity in 509 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). P9WPR1 No conserved hypotheticals P68909 P9WPR1 GO:0015995,GO:0016851,GO:0000166,GO:0006508,GO:0004252,GO:0004176 P9WPR1 NC_000962.3 Mycobrowser_v5 CDS 3207942 3208328 . - 0 Rv2898c Rv2898c Function unknown Conserved hypothetical protein Rv2898c, (MTCY274.29c), len: 128 aa. Conserved hypothetical protein, highly similar to O33024|YS98_MYCLE|ML1607|MLCB250.49 hypothetical 11.0 KDA protein from Mycobacterium leprae (96 aa), FASTA scores: opt: 318, E(): 2.3e-16, (58.35% identity in 96 aa overlap). Also similar to other hypothetical proteins e.g. O69890|YE19_STRCO|SC2E1.19 from Streptomyces coelicolor (130 aa), FASTA scores: opt: 253, E(): 1.7e-11, (39.65% identity in 121 aa overlap); Q9HVZ1|PA4424 from Pseudomonas aeruginosa (125 aa), FASTA scores: opt: 234, E(): 4.2e-10, (40.85% identity in 115 aa overlap); O86871 from Streptomyces lividans (85 aa), FASTA scores: opt: 224, E(): 1.8e-09, (46.45% identity in 84 aa overlap); etc. Equivalent to AAK47292 from Mycobacterium tuberculosis strain CDC1551 (141 aa) but shorter 13 aa. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WFM9 No conserved hypotheticals P67230 P9WFM9 GO:0003677 P9WFM9 NC_000962.3 Mycobrowser_v5 CDS 3209406 3211745 . - 0 Rv2900c fdhF Decomposes formic acid to hydrogen and carbon dioxide under anaerobic conditions in the absence of exogenous electron acceptors [catalytic activity: formate + NAD(+) = CO(2) + NADH]. Possible formate dehydrogenase H FdhF (formate-hydrogen-lyase-linked, selenocysteine-containing polypeptide) (formate dehydrogenase-H alpha subunit) (FDH-H) Rv2900c, (MTCY274.31c), len: 779 aa. Possible fdhF, formate dehydrogenase, highly similar to others formate dehydrogenases and prokaryotic molybdopterin-containing oxidoreductases e.g. Q9S2J9|SC7H2.18 putative formate dehydrogenase from Streptomyces coelicolor (759 aa), FASTA scores: opt: 3038, E(): 2.7e-180, (59.7% identity in 767 aa overlap); Q9HU08|PA5181 probable oxidoreductase from Pseudomonas aeruginosa (773 aa), FASTA scores: opt: 2560, E(): 1.1e-150, (53.2% identity in 761 aa overlap); P78160 formate dehydrogenase a chain (fragment) from Escherichia coli strain K12 (740 aa), FASTA scores: opt: 2002, E(): 3.7e-116, (43.1% identity in 733 aa overlap); P07658|FDHF_ECOLI|P78137|B4079 formate dehydrogenase from Escherichia coli strain K12 (715 aa), FASTA scores: opt: 305, E(): 5.6e-13, (25.5% identity in 748 aa overlap); etc. Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. P9WJP9 No intermediary metabolism and respiration P65408 P9WJP9 GO:0008863,GO:0030151,GO:0055114,GO:0051539 P9WJP9 NC_000962.3 Mycobrowser_v5 CDS 3215665 3216357 . - 0 Rv2906c trmD Specifically methylates guanosime-37 in various TRNAS [catalytic activity S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N1-methylguanine]. Probable tRNA (guanine-N1)-methyltransferase TrmD (M1G-methyltransferase) (tRNA [GM37] methyltransferase) Rv2906c, (MTCY274.37c), len: 230 aa. Probable trmD, tRNA m1G methyltransferase, equivalent to O33017|TRMD_MYCLE from Mycobacterium leprae (238 aa), FASTA scores: opt: 1363, E(): 8.1e-86, (87.2% identity in 227 aa overlap). Also highly similar to others e.g. O69882|TRMD_STRCO from Streptomyces coelicolor and S. lividans (277 aa), FASTA scores: opt: 841, E(): 4.5e-50, (55.55% identity in 234 aa overlap); Q9A0B6 from Streptococcus pyogenes (243 aa), FASTA scores: opt: 698, E(): 2.5e-40, (47.6% identity in 227 aa overlap); P07020|TRMD_ECOLI|TRMD|B2607|Z3901|ECS3470 from Escherichia coli strain O157:H7 (255 aa), FASTA scores: opt: 573, E(): 3.8e-33, (42.1% identity in 228 aa overlap); etc. Belongs to the RNA methyltransferase TRMD family. P9WFY7 No information pathways P66968 P9WFY7 GO:0005737,GO:0009019,GO:0006400,GO:0003723 2.1.1.31 P9WFY7 NC_000962.3 Mycobrowser_v5 CDS 3216361 3216891 . - 0 Rv2907c rimM Essential for efficient processing of 16S rRNA. Probably part of the 30S subunit prior to or during the final step in the processing of 16S free 30S ribosomal subunits. It could be some accessory protein needed for efficient assembly of the 30S subunit. RIMM is needed in a step prior to RBFA during the maturation of 16S rRNA. Has affinity for free ribosomal 30S subunits but not for 70S ribosomes. Probable 16S rRNA processing protein RimM Rv2907c, (MTCY274.38c), len: 176 aa. Probable rimM, 16S rRNA processing protein, equivalent to O33016|RIMM_MYCLE probable 16S rRNA processing protein from Mycobacterium leprae (179 aa), FASTA scores: opt: 797, E(): 2.4e-46, (73.15% identity in 175 aa overlap). Also highly similar to others e.g. O69881|RIMM_STRCO from Streptomyces coelicolor (188 aa), FASTA scores: opt: 485, E(): 2.3e-25, (48.85% identity in 176 aa overlap); Q9KA14|RIMM_BACHD from Bacillus halodurans (173 aa), FASTA scores: opt: 289, E(): 3.2e-12, (30.65% identity in 173 aa overlap); P21504|RIMM_ECOLI|RIMM|B2608 from Escherichia coli strain K12 (182 aa), FASTA scores: opt: 237, E(): 1e-08, (29.4% identity in 177 aa overlap). Belongs to the RimM family. P9WH19 No information pathways P66653 P9WH19 GO:0042274,GO:0005840,GO:0043022,GO:0006364 P9WH19 NC_000962.3 Mycobrowser_v5 CDS 3218339 3219214 . + 0 Rv2911 dacB2 Involved in peptidoglycan synthesis (at final stages). Hydrolyzes the bound D-alanyl-D-alanine [catalytic activity: D-alanyl-D-alanine + H(2)O = 2 D-alanine]. Probable penicillin-binding protein DacB2 (D-alanyl-D-alanine carboxypeptidase) (DD-peptidase) (DD-carboxypeptidase) (PBP) (DD-transpeptidase) (serine-type D-ala-D-ala carboxypeptidase) (D-amino acid hydrolase) Rv2911, (MTCY274.43), len: 291 aa. Probable dacB2, D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein), an ala-rich protein. Highly similar (except in N-terminus) to Q9CCM2|ML0691 putative D-alanyl-D-alanine carboxypeptidase from Mycobacterium leprae (411 aa), FASTA scores: opt: 749, E(): 9.3e-39, (46.75% identity in 276 aa overlap). Also similar to penicillin binding proteins / D-alanyl-D-alanine carboxypeptidases e.g. Q9KCJ8|SC4G1.16c D-alanyl-D-alanine carboxypeptidase from Streptomyces coelicolor (382 aa), FASTA scores: opt: 386, E(): 2.1e-16, (31.25% identity in 285 aa overlap); P35150|DACB_BACSU penicillin-binding protein 5* precursor from Bacillus subtilis (382 aa), FASTA scores: opt: 384, E(): 3.6e-17, (30.7% identity in 244 aa overlap); Q9K8X5|DACB|BH2877 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) from Bacillus halodurans (395 aa), FASTA scores: opt: 359, E(): 9.7e-15, (30.3% identity in 241 aa overlap); P33364|PBP7_ECOLI|PBPG|B2134 penicillin-binding protein 7 precursor from Escherichia coli strain K12 (313 aa), FASTA scores: opt: 273, E(): 7.5e-10, (27.8% identity in 263 aa overlap); etc. Also similar to O53380|Rv3330|MTV016.30 penicillin-binding protein from Mycobacterium tuberculosis (405 aa), FASTA scores: opt: 746, E(): 1.4e-38, (47.0% identity in 266 aa overlap). Seems to contain PF00768 Peptidase_S11 domain PFAM. Belongs to peptidase family S11; also known as the D-alanyl-D-alanine carboxypeptidase 1 family. Thought to be a membrane-bound protein. Note that previously known as dacB. I6Y204 No cell wall and cell processes 2BCF Q7D6F2 I6Y204 GO:0009002,GO:0006508 3.4.16.4 I6Y204 NC_000962.3 Mycobrowser_v5 CDS 3219863 3221698 . - 0 Rv2913c Rv2913c Hydrolyzes specific D-amino acid. Possible D-amino acid aminohydrolase (D-amino acid hydrolase) Rv2913c, (MTCY338.01c, MTCY274.45c), len: 611 aa. Possible D-amino acid aminohydrolase, similar (principally in N-terminus) to D-amino acid aminohydrolases e.g. Q9V2D3|NDAD|PAB0090 D-aminoacylase (aspartate, glutamate etc) from Pyrococcus abyssi (526 aa), FASTA scores: opt: 336, E(): 2.2e-13, (27.55% identity in 581 aa overlap); P94212|NDDD_ALCXX N-acyl-D-aspartate deacylase (N-acyl-D-aspartate amidohydrolase) from Alcaligenes xylosoxydans xylosoxydans (Achromobacter xylosoxidans) (498 aa), FASTA scores: opt: 221, E(): 3.4e-06, (25.95% identity in 532 aa overlap); Q9AGH8 D-aminoacylase from Alcaligenes faecalis (484 aa), FASTA scores: opt: 218, E(): 5.1e-06, (28.35% identity in 434 aa overlap); etc. P9WJH9 No intermediary metabolism and respiration P65530 P9WJH9 GO:0016810 P9WJH9 NC_000962.3 Mycobrowser_v5 CDS 3385163 3386077 . - 0 Rv3026c Rv3026c Function unknown Conserved hypothetical protein Rv3026c, (MTV012.41c), len: 304 aa. Conserved hypothetical protein, similar to Q9RCZ0|SCM10.08C putative acyltransferase from Streptomyces coelicolor (275 aa), FASTA scores: opt: 393, E(): 2.2e-17, (41.4% identity in 299 aa overlap). Similar in part to other hypothetical proteins and acyltransferases e.g. BAB51968|MLR5533 from Rhizobium loti (266 aa), FASTA scores: opt: 280, E(): 2.4e-10, (29.45% identity in 258 aa overlap); Q9KIH9 putative acyltransferase (putative acyltransferase transmembrane protein) from Rhizobium meliloti (Sinorhizobium meliloti) (292 aa), FASTA scores: opt: 252, E(): 1.4e-08, (30.5% identity in 210 aa overlap); O69114|PLSC putative 1-acyl-SN-glycerol-3-phosphate acyltransferase from Burkholderia pseudomallei (Pseudomonas pseudomallei) (289 aa), FASTA scores: opt: 216, E(): 2.4e-06, (30.85% identity in 269 aa overlap); etc. So may be a member of acyltransferase family protein. I6XFY8 No conserved hypotheticals O53273 I6XFY8 GO:0008152,GO:0008415 I6XFY8 NC_000962.3 Mycobrowser_v5 CDS 3226363 3228243 . + 0 Rv2917 Rv2917 Function unknown Conserved hypothetical alanine and arginine rich protein Rv2917, (MTCY338.05), len: 626 aa. Conserved hypothetical ala-, arg-rich protein, highly similar (but longer 34 aa) to O33011|ML1624|MLCB250.18C hypothetical 65.2 KDA protein from Mycobacterium leprae (596 aa), FASTA scores: opt: 3117, E(): 9e-183, (79.8% identity in 584 aa overlap). Also highly similar to Q9S2E8|SCE19A.36C hypothetical 66.2 KDA protein from Streptomyces coelicolor (598 aa), FASTA scores: opt: 1921, E(): 1.1e-109, (56.08% identity in 567 aa overlap); and Q9S3Y6|SDRA SDRA protein from Streptomyces coelicolor (597 aa), FASTA scores: opt: 1896, E(): 3.6e-108, (55.75% identity in 567 aa overlap). And shows some similarity with others proteins from other organisms. Equivalent to AAK47311 putative RNA helicase from Mycobacterium tuberculosis strain CDC1551 (602 aa) but longer 24 aa. Contains PS00017 ATP/GTP-binding site motif A (P-loop). P9WL21 No conserved hypotheticals Q10966 P9WL21 GO:0003677,GO:0017111,GO:0005524 P9WL21 NC_000962.3 Mycobrowser_v5 CDS 3228254 3230680 . - 0 Rv2918c glnD Modifies, by uridylylation or deuridylylation the PII (GLNB|Rv2919c) regulatory protein [catalytic activity: UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII]]. Probable [protein-PII] uridylyltransferase GlnD (PII uridylyl- transferase) (uridylyl removing enzyme) (UTASE) Rv2918c, (MTCY338.07c), len: 808 aa. Probable glnD, uridylyltransferase (ala-rich protein), similar to other uridylyltransferases e.g. O69873||SC2E1.02 from Streptomyces coelicolor (835 aa), FASTA scores: opt: 1473, E(): 2.8e-81, (41.03% identity in 858 aa overlap); P43919|GLND_HAEIN from Haemophilus influenzae (863 aa), FASTA scores: opt: 333, E(): 2.5e-12, (25.4% identity in 819 aa overlap); P27249|GLND_ECOLI|GLND|B0167 from Escherichia coli strain K12 (890 aa), FASTA scores: opt: 306, E(): 1.1e-10, (27.75% identity in 858 aa overlap); etc. Belongs to the GlnD family. P9WN29 No intermediary metabolism and respiration Q10961 P9WN29 GO:0016597,GO:0006807,GO:0008773 2.7.7.59 P9WN29 NC_000962.3 Mycobrowser_v5 CDS 3231073 3232506 . - 0 Rv2920c amt Thought to be involved in transport of ammonium across the membrane (export). Responsible for the translocation of the substrate across the membrane. Probable ammonium-transport integral membrane protein Amt Rv2920c, (MTCY338.09c), len: 477 aa. Probable amt, ammonium-transport integral membrane protein (ala-, gly-, leu-, val-rich protein), highly similar to others e.g. Q9ZBP6|SC7A1.27 ammonium transporter from Streptomyces coelicolor (448 aa), FASTA scores: opt: 1246, E(): 7.3e-67, (54.1% identity in 462 aa overlap); P54146|AMT_CORGL ammonium transport system from Corynebacterium glutamicum (452 aa), FASTA scores: opt: 953, E(): 2.1e-49, (41.45% identity in 475 aa overlap); Q07429|NRGA_BACSU probable ammonium transporter (membrane protein NRGA) from Bacillus subtilis (404 aa), FASTA scores: opt: 721, E(): 0, (44.4% identity in 430 aa overlap); etc. Belongs to the AMT1/MEP/NRGA family of ammonium transporters (TC 2.49). P9WQ65 No cell wall and cell processes P63519 P9WQ65 GO:0015696,GO:0016021,GO:0005886,GO:0055085,GO:0008519 P9WQ65 NC_000962.3 Mycobrowser_v5 CDS 3232871 3234139 . - 0 Rv2921c ftsY Probably involved in the reception and insertion of a subset of proteins at the membrane: possibly membrane receptor for FFH|Rv2916c. Probable cell division protein FtsY (SRP receptor) (signal recognition particle receptor) Rv2921c, (MTCY338.10c, MT2989), len: 422 aa. Probable ftsY, signal recognition particle (SRP) receptor, a membrane-associated cell division protein (see citation below), equivalent to O33010|FTSY_MYCLE cell division protein FTSY homolog from Mycobacterium leprae (430 aa), FASTA scores: opt: 1760, E(): 1.1e-108, (81.35% identity in 429 aa overlap). Also similar to others e.g. Q9I6C1|FTSY|PA0373 signal recognition particle receptor FTSY from Pseudomonas aeruginosa (455 aa), FASTA scores: opt: 882, E(): 5.1e-40, (42.08% identity in 385 aa overlap); Q9KVJ6|FTSY cell division protein from Vibrio cholerae (391 aa), FASTA scores: opt: 837, E(): 1.2e-37, (36.3% identity in 394 aa overlap); P10121|FTSY_ECOLI|FTSY|B3464 cell division protein from Escherichia coli strain K12 (497 aa), FASTA scores: opt: 800, E(): 1.3e-35, (39.75% identity in 327 aa overlap); etc. Also similar to Q9ZBP9|SC7A1.24 putative prokaryotic docking protein from Streptomyces coelicolor (412 aa), FASTA scores: opt: 1461, E(): 4.3e-71, (60.3% identity in 423 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00300 SRP54-type proteins GTP-binding domain signature. Belongs to the SRP family of GTP-binding proteins. P9WGD9 No cell wall and cell processes P66842 P9WGD9 GO:0003723,GO:0006614,GO:0007049,GO:0051301,GO:0017111,GO:0005886,GO:0005515,GO:0030529,GO:0005525 P9WGD9 NC_000962.3 Mycobrowser_v5 CDS 3241222 3241959 . - 0 Rv2927c Rv2927c Function unknown Conserved hypothetical protein Rv2927c, (MTCY338.16c), len: 245 aa. Conserved hypothetical protein, equivalent to Q9CBS6|ML1661|MLCB1243.13 (alias O69467) hypothetical protein from Mycobacterium leprae (247 aa), FASTA scores: opt: 1440, E(): 4.9e-76, (90.6% identity in 245 aa overlap). Also similar to many hypothetical proteins from other organisms e.g. Q9ZBR0|SC7A1.13 hypothetical 41.0 KDA protein from Streptomyces coelicolor (379 aa), FASTA scores: opt: 266, E(): 3.4e-08, (29.9% identity in 234 aa overlap); etc. Also some similarity with P46815|AG84_MYCLE|ML0922 antigen 84 from Mycobacterium leprae (266 aa), FASTA scores: opt: 193, E(): 0.00043, (28.7% identity in 136 aa overlap) (see citation below); and P46816|AG84_MYCTU|WAG31|Rv2145c|MT2204|MTCY270.23 antigen 84 from Mycobacterium tuberculosis (260 aa), FASTA scores: opt: 178, E(): 0.0031, (34.35% identity in 131 aa overlap) (see citation below). Contains potential coiled-coil region. P9WL15 No conserved hypotheticals P9WL15 P9WL15 NC_000962.3 Mycobrowser_v5 CDS 3272214 3273209 . + 0 Rv2936 drrA Probably involved in active transport of antibiotic and phthiocerol dimycocerosate (dim) across the membrane (export). DRRA, DRRB|Rv2936|MTCY19H9.05 and DRRC|Rv2938|MTCY19H9.06 may act jointly to confer daunorubicin and doxorubicin resistance by an export mechanism. Responsible for energy coupling to the transport system. Daunorubicin-dim-transport ATP-binding protein ABC transporter DrrA Rv2936, (MTCY19H9.04), len: 331 aa. drrA, daunorubicin-dim-transport resistance ATP-binding protein ABC transporter, probably involved in daunorubicin resistance and phthiocerol dimycocerosate transport (see citations below), equivalent to Q49938|DRRA|ML2352|L518_F2_43|DRRA probable daunorubicin resistance ATP-binding protein from Mycobacterium leprae (331 aa), FASTA scores: opt: 1842, E(): 4.2e-103, (85.2% identity in 331 aa overlap). Also highly similar to others e.g. Q9XCF7 DRRA from Mycobacterium avium (315 aa), FASTA scores: opt: 1040, E(): 4.7e-55, (54.35% identity in 309 aa overlap); Q9X5J8 daunorubicin resistance protein A from Mycobacterium avium (315 aa), FASTA scores: opt: 1030, E(): 1.9e-54, (53.7% identity in 309 aa overlap); P32010|DRRA_STRPE daunorubicin resistance ATP-binding protein from Streptomyces peucetius (330 aa), FASTA scores: opt: 852, E(): 9e-44, (47.15% identity in 318 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). Note that Rv2936|drrA belongs to the transcriptional unit Rv2930|fadD26-Rv2939|papA5 (proven experimentally). P9WQL9 No cell wall and cell processes P96205 P9WQL9 GO:0016887,GO:0005886,GO:0046677,GO:0055085,GO:0005524 3.6.3.- P9WQL9 NC_000962.3 Mycobrowser_v5 CDS 3274072 3274902 . + 0 Rv2938 drrC Probably involved in active transport of antibiotic and phthiocerol dimycocerosate (dim) across the membrane (export). DRRA|Rv2934|MTCY19H9.04, DRRB|Rv2937|MTCY19H9.05 and DRRC may act jointly to confer daunorubicin and doxorubicin resistance by an export mechanism. Probably responsible for the translocation of the substrate across the membrane and localization of dim into the cell wall. Probable daunorubicin-dim-transport integral membrane protein ABC transporter DrrC Rv2938, (MTCY19H9.06), len: 276 aa. Probable drrC, daunorubicin-dim-transport integral membrane protein ABC transporter, probably involved in daunorubicin resistance and phthiocerol dimycocerosate transport (see citations below), equivalent to Q9CB71|ML2350 probable antibiotic resistance membrane protein from Mycobacterium leprae (276 aa), FASTA scores: opt: 1434, E(): 1.2e-81, (79.0% identity in 276 aa overlap); and Q49941|DRRC|L518_F3_76 putative daunorubicin resistance transmembrane protein from Mycobacterium leprae (244 aa), FASTA scores: opt: 1194, E(): 8.3e-67, (76.85% identity in 242 aa overlap). Also similar to others e.g. Q9XCF9 DRRC protein from Mycobacterium avium (263 aa), FASTA scores: opt: 538, E(): 3.7e-26, (32.65% identity in 251 aa overlap); Q9S6H3 daunorubicin resistance protein C from Mycobacterium avium (263 aa), FASTA scores: opt: 533, E(): 7.6e-26, (32.25% identity in 251 aa overlap); P32011|DRRB_STRPE daunorubicin resistance transmembrane protein from Streptomyces peucetius (283 aa), FASTA scores: opt: 276, E(): 6.6e-10, (21.07% identity in 261 aa overlap); etc. Note that Rv2938|drrC belongs to the transcriptional unit Rv2930|fadD26-Rv2939|papA5 (proven experimentally). P9WG21 No cell wall and cell processes P96207 P9WG21 GO:0042626,GO:0016021,GO:0005886,GO:0046677,GO:0006810,GO:0005524 NC_000962.3 Mycobrowser_v5 CDS 3283335 3285077 . + 0 Rv2941 fadD28 Involved in phthiocerol dimycocerosate (dim) biosynthesis. Thought to be involved in the release and transfer of mycoserosic acid from mas onto the DIOLS. Fatty-acid-AMP ligase FadD28 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) Rv2941, (MTCY24G1.08c), len: 580 aa. FadD28 (alternate gene name: acoas), fatty-acid-AMP synthetase (see citations below), almost identical to P71495 acyl-CoA synthase from Mycobacterium bovis (582 aa), FASTA scores: opt: 3828, E(): 0, (99.15% identity in 580 aa overlap); and equivalent to Q9CD79|FADD28|ML0138 acyl-CoA synthetase from Mycobacterium leprae (579 aa), FASTA scores: opt: 3183, E(): 8.8e-186, (81.9% identity in 580 aa overlap). And also highly similar to others Mycobacteria proteins e.g. O07797|FADD23|Rv3826|MTCY409.04c putative fatty-acid-CoA synthetase from Mycobacterium tuberculosis (584 aa); etc. Contains PS00018 EF-hand calcium-binding domain. Note that Rv2941|fadD28 and Rv2942|mmpL7 are transcriptionally coupled (proven experimentally). P9WQ59 No lipid metabolism 3E53 P96290 P9WQ59 GO:0008152,GO:0016874 6.2.1.- P9WQ59 NC_000962.3 Mycobrowser_v5 CDS 3350274 3350993 . - 0 Rv2993c Rv2993c Isomerizes HHDD (2-hydroxy-hept-2,4-diene-1,7-dioate) to OHED (2-oxo-hept-3-ene-1,7-dioate). Possible 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (HHDD isomerase) Rv2993c, (MTV012.07c), len: 239 aa. Possible 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, equivalent to O33119|ML1689|MLCB637.28 possible 2-hydroxyhepta-2,4-diene- 1,7-dioate isomerase from Mycobacterium leprae (242 aa), FASTA scores: opt: 1427, E(): 4.4e-86, (85.9% identity in 241 aa overlap). Also similar to others e.g. Q9LBE3|DR1609 from Deinococcus radiodurans (250 aa), FASTA scores: opt: 723, E(): 5.5e-40, (49.05% identity in 216 aa overlap); O27551|MTH1507 from Methanothermobacter thermautotrophicus (260 aa), FASTA scores: opt: 708, E(): 5.4e-39, (52.1% identity in 213 aa overlap); Q9HQR6|VNG1037G|HPCE from Halobacterium sp. (strain NRC-1) (244 aa), FASTA scores: opt: 590, E(): 2.7e-31, (43.65% identity in 220 aa overlap); etc. Start chosen by homology, but ORF could continue upstream. I6Y276 No intermediary metabolism and respiration O53242 I6Y276 GO:0008152,GO:0016853 5.3.3.- I6Y276 NC_000962.3 Mycobrowser_v5 CDS 3290624 3291325 . - 0 Rv2945c lppX Unknown Probable conserved lipoprotein LppX Rv2945c, (MTCY24G1.04), len: 233 aa. Probable lppX, conserved lipoprotein, equivalent to Q9CD80 putative lipoprotein from Mycobacterium leprae (233 aa), FASTA scores: opt: 1165, E(): 2.1e-65, (76.4% identity in 233 aa overlap); and similar to Q9CCP6|ML0557 from Mycobacterium leprae (238 aa), FASTA scores: opt: 338, E(): 7.4e-14, (30.75% identity in 231 aa overlap). Also similar to others from Mycobacterium tuberculosis e.g. P71679|LPRG_MYCTU lipoprotein (236 aa), FASTA scores: opt: 342, E(): 4.1e-14, (32.05% identity in 231 aa overlap); etc. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site, and has in its N-terminal a signal peptide. Belongs to the LPPX/lprafg family of lipoproteins. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WK65 No cell wall and cell processes 2BYO P65306 P9WK65 GO:0005886 P9WK65 NC_000962.3 Mycobrowser_v5 CDS 3297837 3299954 . - 0 Rv2948c fadD22 Involved in biosynthesis of phenolic glycolipids (PGLs) P-hydroxybenzoyl-AMP ligase FadD22 Rv2948c, (MTCY24G1.01), len: 705 aa. FadD22, p-hydroxybenzoyl-AMP ligase. Highly similar to many e.g. Q9CD82|ML0134 putative acyl-CoA synthetase from Mycobacterium leprae (707 aa), fasta scores: opt: 3554, E(): 6.4e-209, (75.9% identity in 705 aa overlap). Almost identical to G560509|Q50468 PKS002A protein from Mycobacterium tuberculosis (705 aa), fasta scores: opt: 4647, E(): 0, (99.7% identity in 705 aa overlap). P9WQ61 No lipid metabolism P96283 P9WQ61 GO:0048037,GO:0016874,GO:0008152,GO:0000036 6.2.1.- P9WQ61 NC_000962.3 Mycobrowser_v5 CDS 3300596 3302455 . - 0 Rv2950c fadD29 Involved in biosynthesis of phenolic glycolipids (PGLs) Fatty-acid-AMP ligase FadD29 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) Rv2950c, (MTCY349.40), len: 619 aa. fadD29, fatty-acid-AMP synthetase, similar to various mycobacterial enzymes believed to be involved in polyketide or fatty acid synthesis. Equivalent (but shorter 61 aa) to Q9CD84 from Mycobacterium leprae (680 aa), FASTA scores: opt: 3280, E(): 2.2e-192, (80.15% identity in 620 aa overlap); and highly similar to others from Mycobacterium leprae e.g. Q9Z5K5 probable acyl-CoA synthase (583 aa), FASTA scores: opt: 2358, E(): 3.4e-136, (62.35% identity in 579 aa overlap). Also similar to others from Mycobacterium tuberculosis e.g. Q10976|FD26_MYCTU putative fatty-acid--CoA ligase (583 aa), FASTA scores: opt: 2416, E(): 1e-139, (63.15% identity in 581 aa overlap) (N-terminus shorter); etc. Equivalent to AAK47349 from Mycobacterium tuberculosis strain CDC1551 (582 aa) but longer 37 aa. P95141 No lipid metabolism P95141 P95141 GO:0008152,GO:0016874 6.2.1.- P95141 NC_000962.3 Mycobrowser_v5 CDS 3304441 3305253 . + 0 Rv2952 Rv2952 Thought to cause methylation. Possible methyltransferase (methylase) Rv2952, (MTCY349.38), len: 270 aa. Probable methyltransferase, equivalent to Q9CD86|ML0130 hypothetical protein from Mycobacterium leprae (270 aa), FASTA scores: opt: 1584, E(): 6.1e-99, (83.7% identity in 270 aa overlap). Also highly similar to Q9RMN9|MTF2 putative methyltransferase from Mycobacterium smegmatis (274 aa), FASTA scores: opt: 902, E(): 3.8e-53, (56.35% identity in 252 aa overlap). Also similar to other methyltransferases e.g. Q9ADL4|SORM O-methyltransferase from Polyangium cellulosum (346 aa), FASTA scores: opt: 390, E(): 1.1e-18, (36.25% identity in 251 aa overlap); Q54303|RAPM methyltransferase from Streptomyces hygroscopicus (317 aa), FASTA scores: opt: 315, E(): 1.1e-13, (40.75% identity in 135 aa overlap); etc. Very similar to C-terminal part of Q50584|Rv1523|MTCY19G5.05c hypothetical 37.9 KDA protein from Mycobacterium tuberculosis (358 aa), FASTA score: opt: 965, E(): 2.7e-57, (60.3% identity in 247 aa overlap). P9WIN3 No intermediary metabolism and respiration Q50464 P9WIN3 GO:0008168,GO:0008610 2.1.1.- P9WIN3 NC_000962.3 Mycobrowser_v5 CDS 3310714 3312000 . - 0 Rv2958c Rv2958c Function unknown; probably involved in cellular metabolism. Possibly involved in resistance to killing by human macrophages. Possible glycosyl transferase Rv2958c, (MTCY349.30), len: 428 aa. Possible glycosyl transferase (see citation below), highly similar to Q9CD88|ML0128 putative glycosyl transferase from Mycobacterium leprae (435 aa), FASTA scores: opt: 2116, E(): 5.8e-126, (75.05% identity in 417 aa overlap); and Q9CD91|ML0125 putative glycosyl transferase from Mycobacterium leprae (438 aa), FASTA scores: opt: 2104, E(): 3.3e-125, (74.65% identity in 418 aa overlap). Also shows some similarity to variety of glycosyl transferases e.g. Q9RYI3 putative glycosyltransferase from Deinococcus radiodurans (418 aa), FASTA scores: opt: 317, E(): 1.9e-12, (31.0% identity in 297 aa overlap); Q9S1V2 putative glycosyl transferase from Streptomyces coelicolor (407 aa), FASTA scores: opt: 264, E(): 4.1e-09, (27.2% identity in 342 aa overlap); P72650|CRTX|SLR1125 zeaxanthin glucosyl transferase from Synechocystis sp. strain PCC 6803 (419 aa), FASTA scores: opt: 251, E(): 2.8e-08, (26.8% identity in 295 aa overlap); etc. Very similar to P95130|MTCY349.25 from Mycobacterium tuberculosis (449 aa), FASTA score: opt: 2215, E(): 3.3e-132, (77.25% identity in 422 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WFR1 No intermediary metabolism and respiration P95134 P9WFR1 GO:0016758,GO:0008152 2.4.1.- P9WFR1 NC_000962.3 Mycobrowser_v5 CDS 3313773 3315122 . - 0 Rv2962c Rv2962c Function unknown; probably involved in cellular metabolism. Possibly involved in resistance to killing by human macrophages. Possible glycosyl transferase Rv2962c, (MTCY349.25), len: 449 aa. Possible glycosyl transferase (see citation below), highly similar or identical to Mycobacterium tuberculosis proteins G560522 U0002JA, G560521 U0002H, G560522 U0002JA, G560519 U0002KA. Equivalent (but longer 21 aa) to Q9CD91 putative glycosyl transferase from Mycobacterium leprae (438 aa), FASTA scores: opt: 2229, E(): 1.3e-133, (77.45% identity in 426 aa overlap); and highly similar to Q9CD88 putative glycosyl transferase from Mycobacterium leprae (435 aa), FASTA scores: opt: 2129, E(): 2.7e-127, (74.35% identity in 425 aa overlap); and others from Mycobacterium leprae. Also shows some similarity to variety of glycosyl transferases e.g. Q9RYI3|DRA0329 putative glycosyl transferase from Deinococcus radiodurans (418 aa), FASTA scores: opt: 340, E(): 5.5e-14, (31.2% identity in 330 aa overlap); P72650 zeaxanthin glucosyl transferase from Synechocystis sp. (strain PCC 6803) (419 aa), FASTA scores: opt: 244, E(): 6.6e-08, (26.2% identity in 294 aa overlap); etc. Also highly similar to P95134 hypothetical 46.8 KDA protein from Mycobacterium tuberculosis (428 aa), FASTA scores: opt: 2215, E(): 9.6e-133, (77.25% identity in 422 aa overlap). P9WN09 No intermediary metabolism and respiration P95130 P9WN09 GO:0016758,GO:0008152 2.4.1.- P9WN09 NC_000962.3 Mycobrowser_v5 CDS 3316529 3317461 . + 0 Rv2964 purU Involved in de novo purine biosynthesis [catalytic activity: 10-formyltetrahydrofolate + H(2)O = formate + tetrahydrofolate]. Probable formyltetrahydrofolate deformylase PurU (formyl-FH(4) hydrolase) Rv2964, (MTCY349.23c), len: 310 aa. Probable purU, formyltetrahydrofolate deformylase, highly similar to others e.g. Q9RWT1|DR0584 formyltetrahydrofolate deformylase from Deinococcus radiodurans (298 aa), FASTA scores: opt: 1005, E(): 4.9e-52, (52.25% identity in 297 aa overlap); Q9K7U4 formyltetrahydrofolate deformylase from Bacillus halodurans (289 aa), FASTA scores: opt: 982, E(): 1.1e-50, (51.8% identity in 280 aa overlap); Q55135|PURU_SYNY3|SLL0070 formyltetrahydrofolate deformylase from Synechocystis sp. strain PCC 6803 (284 aa), FASTA scores: opt: 839, E(): 2.9e-42, (48.2% identity in 280 aa overlap); etc. P9WHM3 No intermediary metabolism and respiration P0A5T6 P9WHM3 GO:0016597,GO:0008864,GO:0016742,GO:0006730,GO:0006189 3.5.1.10 P9WHM3 NC_000962.3 Mycobrowser_v5 CDS 3319663 3323046 . - 0 Rv2967c pca Involved in gluconeogenesis and lipogenesis. Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [catalytic activity: ATP + pyruvate + HCO(3)(-) = ADP + phosphate + oxaloacetate]. Probable pyruvate carboxylase Pca (pyruvic carboxylase) Rv2967c, (MTCY349.20), len: 1127 aa. Probable pca, pyruvate carboxylase (ala-rich protein), equivalent to Q9F843|PYC pyruvate carboxylase from Mycobacterium smegmatis (1127 aa), FASTA scores: opt: 6232, E(): 0, (83.3% identity in 1127 aa overlap). Also highly similar to others e.g. Q9RK64|SCF11.26c pyruvate carboxylase from Streptomyces coelicolor (1124 aa), FASTA scores: opt: 5526, E(): 0, (74.65% identity in 1125 aa overlap); O54587|PYC pyruvate carboxylase from Corynebacterium glutamicum (Brevibacterium flavum) (1140 aa), FASTA scores: opt: 4811, E(): 0, (64.5% identity in 1132 aa overlap); Q9DDT1 pyruvate carboxylase from Brachydanio rerio (Zebrafish) (1180 aa), FASTA scores: opt: 3133, E(): 1.1e-171, (47.8% identity in 1142 aa overlap); etc. Contains PS00867 Carbamoyl-phosphate synthase subdomain signature 2, PS00165 Serine/threonine dehydratases pyridoxal-phosphate attachment site, and PS00188 Biotin-requiring enzymes attachment site. I6YEU0 No intermediary metabolism and respiration P95127 I6YEU0 GO:0009374,GO:0005737,GO:0006094,GO:0004736,GO:0005524 6.4.1.1 I6YEU0 NC_000962.3 Mycobrowser_v5 CDS 3323709 3324476 . - 0 Rv2969c Rv2969c Unknown Possible conserved membrane or secreted protein Rv2969c, (MTCY349.18), len: 255 aa. Possible conserved membrane or exported protein, equivalent to Q9CBS4|ML1667 possible conserved membrane protein from Mycobacterium leprae (264 aa), FASTA scores: opt: 1101, E(): 9.9e-68, (65.9% identity in 258 aa overlap); and highly similar to O69463 putative transmembrane protein from Mycobacterium leprae (258 aa), FASTA scores: opt: 1097, E(): 1.8e-67, (65.5% identity in 258 aa overlap). C-terminus also highly similar to Q9KK65|996A160 exported protein (fragment) from Mycobacterium avium (85 aa), FASTA scores: opt: 418, E(): 2e-21, (72.95% identity in 85 aa overlap). Also weakly similar to membrane or exported proteins e.g. Q9S2U7|SC4G6.04c putative integral membrane protein from Streptomyces coelicolor (275 aa), FASTA scores: opt: 312, E(): 7.6e-14, (28.25% identity in 230 aa overlap); Q9XAB6|SCC22.22C putative secreted protein from Streptomyces coelicolor (255 aa), FASTA scores: opt: 181, E(): 6.4e-05, (27.0% identity in 226 aa overlap); etc. Also some similarity with P72001|PKNE_MYCTU from Mycobacterium tuberculosis (566 aa), FASTA scores: opt: 264, E(): 2.3e-10, (30.5% identity in 177 aa overlap). O33272 No cell wall and cell processes O33272 O33272 NC_000962.3 Mycobrowser_v5 CDS 3326101 3326949 . + 0 Rv2971 Rv2971 Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv2971, (MTCY349.16c), len: 282 aa. Probable oxidoreductase, possibly aldo/keto reductase, equivalent to O69462 putative oxidoreductase from Mycobacterium leprae (282 aa), FASTA scores: opt: 1495, E(): 4.9e-93, (82.35% identity in 272 aa overlap). Also similar to others e.g. Q9KYM9|SC9H11.10C oxidoreductase from Streptomyces coelicolor (276 aa), FASTA scores: opt: 849, E(): 1.2e-49, (51.7% identity in 267 aa overlap); Q9ZBW7|SC4B5.01C putative oxidoreductase from Streptomyces coelicolor (277 aa), FASTA scores: opt: 847, E(): 1.7e-49, (49.1% identity in 271 aa overlap); Q46857|YQHE_ECOLI|YQHE|B3012 hypothetical oxidoreductase from Escherichia coli strain K12 (275 aa), FASTA scores: opt: 827, E(): 3.7e-48, (47.45% identity in 276 aa overlap); etc. Contains PS00063 Aldo/keto reductase family putative active site signature; and PS00062 Aldo/keto reductase family signature 2. P9WQA5 No intermediary metabolism and respiration P95124 P9WQA5 GO:0016491,GO:0055114 1.-.-.- P9WQA5 NC_000962.3 Mycobrowser_v5 CDS 3327023 3327736 . - 0 Rv2972c Rv2972c Unknown Possible conserved membrane or exported protein Rv2972c, (MTCY349.15), len: 237 aa. Possible conserved membrane or exported protein, equivalent (but longer 52 aa) to O69461|MLCB1243.02 hypothetical 20.5 KDA protein from Mycobacterium leprae (180 aa), FASTA scores: opt: 581, E(): 8.2e-32, (55.75% identity in 174 aa overlap). Also similar to membrane or exported proteins e.g. Q9F2P3|SCE41.16C putative lipoprotein from Streptomyces coelicolor (258 aa), FASTA scores: opt: 498, E(): 4.1e-26, (44.08% identity in 186 aa overlap); Q99QB5|SCP1.323C putative secreted protein from Streptomyces coelicolor (219 aa), FASTA scores: opt: 329, E(): 8.5e-15, (36.35% identity in 176 aa overlap); Q9ACQ1|SCP1.267 putative secreted protein from Streptomyces coelicolor (219 aa), FASTA scores: opt: 286, E(): 6.6e-12, (32.03% identity in 231 aa overlap); etc. I6X5W6 No cell wall and cell processes P95123 I6X5W6 I6X5W6 NC_000962.3 Mycobrowser_v5 CDS 3332071 3332754 . - 0 Rv2976c ung Involved in base excision repair.excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. Probable uracil-DNA glycosylase Ung (UDG) Rv2976c, (MTCY349.11), len: 227 aa. Probable ung, uracil-DNA glycosylase (see citation below), equivalent to Q9CBS3 uracil-DNA glycosylase from Mycobacterium leprae (227 aa), FASTA scores: opt: 1394, E(): 8.8e-85, (88.1% identity in 227 aa overlap). Also highly similar to others e.g. Q9EX12 from Streptomyces coelicolor (225 aa), FASTA scores: opt: 1134, E(): 1.3e-67, (72.75% identity in 224 aa overlap); Q9K682|UNG_BACHD from Bacillus halodurans (224 aa), FASTA scores: opt: 652, E(): 8.9e-36, (45.5% identity in 222 aa overlap); P39615|UNG_BACSU from Bacillus subtilis (225 aa), FASTA scores: opt: 625, E(): 5.4e-34, (45.5% identity in 222 aa overlap); etc. Belongs to the uracil-DNA glycosylase family. P9WFQ9 No information pathways 2ZHX,3A7N P67071 P9WFQ9 GO:0005737,GO:0004844,GO:0006284 3.2.2.27 P9WFQ9 NC_000962.3 Mycobrowser_v5 CDS 3332787 3333788 . - 0 Rv2977c thiL Involved in thiamine biosynthesis [catalytic activity: ATP + thiamine phosphate = ADP + thiamine diphosphate]. Probable thiamine-monophosphate kinase ThiL (thiamine-phosphate kinase) Rv2977c, (MTCY349.10), len: 333 aa. Possible thiL, thiamin-monophosphate kinase, equivalent to Q9CBS2 probable thiamine-monophosphate kinase from Mycobacterium leprae (325 aa), FASTA scores: opt: 1738, E(): 4.5e-98, (80.9% identity in 314 aa overlap). Also highly similar to others e.g. Q9ZBR7|SC7A1.06 putative thiamine monphosphate kinase from Streptomyces coelicolor (322 aa), FASTA scores: opt: 959, E(): 7.8e-51, (51.1% identity in 319 aa overlap); O05514|THIL_BACSU thiamine-monophosphate kinase from Bacillus subtilis (325 aa), FASTA scores: opt: 476, E(): 1.5e-21, (35.15% identity in 273 aa overlap); P77785|THIL_ECOLI|THIL|B0417 thiamine-monophosphate kinase from Escherichia coli strain K12 (325 aa), FASTA scores: opt: 418, E(): 5e-18, (36.9% identity in 282 aa overlap); etc. Belongs to the thiamine-monophosphate kinase family. Note that the start, as given, is in IS1538. P9WG71 No intermediary metabolism and respiration P95118 P9WG71 GO:0009030,GO:0009228 2.7.4.16 P9WG71 NC_000962.3 Mycobrowser_v5 CDS 3336796 3337917 . - 0 Rv2981c ddlA Involved in cell wall formation. Along with alanine racemase, it MAKES up the D-alanine branch of the peptidoglycan biosynthetic route. [catalytic activity: ATP + D-alanine + D-alanine = ADP + phosphate + D-alanyl-D-alanine]. Probable D-alanine--D-alanine ligase DdlA (D-alanylalanine synthetase) (D-ala-D-ala ligase) Rv2981c, (MTCY349.06), len: 373 aa. Probable ddlA (alternate gene name: ddl), D-alanine--D-alanine ligase a (see citation below), equivalent to Q9CBS0|Q9CBS0 D-alanine-D-alanine ligase a from Mycobacterium leprae (384 aa), FASTA scores: opt: 2001, E(): 2.4e-115, (81.75% identity in 367 aa overlap); and Q9ZGN0|DDL_MYCSM D-alanine--D-alanine ligase from Mycobacterium smegmatis (373 aa), FASTA scores: opt: 1934, E(): 3.1e-111, (77.95% identity in 372 aa overlap). Also highly similar to others e.g. Q9ZBR9|DDL_STRCO from Streptomyces coelicolor (389 aa), FASTA scores: opt: 1187, E(): 2.2e-65, (52.0% identity in 379 aa overlap); P15051|DDLA_SALTY from Salmonella typhimurium and Salmonella typhi (363 aa), FASTA scores: opt: 946, E(): 1.3e-50, (44.5% identity in 364 aa overlap); P23844|DDLA_ECOLI|DDLA|B0381|Z0477|ECS0431 from Escherichia coli strain O157:H7 and K12 (364 aa), FASTA scores: opt: 938, E(): 3.9e-50, (43.55% identity in 363 aa overlap); etc. Contains PS00843 D-alanine--D-alanine ligase signature 1. Belongs to the D-alanine--D-alanine ligase family. P9WP31 No cell wall and cell processes 3LWB P95114 P9WP31 GO:0008716,GO:0005618,GO:0007047,GO:0005737,GO:0046872,GO:0009252,GO:0008360,GO:0005524 6.3.2.4 P9WP31 NC_000962.3 Mycobrowser_v5 CDS 3339854 3342082 . + 0 Rv2984 ppk1 Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyp) [catalytic activity: ATP + {phosphate}(N) = ADP + {phosphate}(N+1)]. Likely involved in MPRAB-SIGE-rel signalling and the stringent response. Polyphosphate kinase PPK (polyphosphoric acid kinase) (ATP-polyphosphate phosphotransferase) Rv2984, (MTCY349.03c), len: 742 aa. Ppk1, polyphosphate kinase (See Sureka et al., 2007), equivalent to O33127|PPK_MYCLE polyphosphate kinase from Mycobacterium leprae (739 aa), FASTA scores: opt: 4264, E(): 0, (87.85% identity in 742 aa overlap). Also highly similar to others e.g. Q9KZV6|PPK_STRCO from Streptomyces coelicolor (746 aa), FASTA scores: opt: 1979, E(): 2.6e-117, (59.9% identity in 701 aa overlap); Q9KD27|PPK_BACHD from Bacillus halodurans (705 aa), FASTA scores: opt: 1319, E(): 1.4e-75, (45.55% identity in 674 aa overlap); Q9PAC7|PPK_XYLFA from Xylella fastidiosa (698 aa), FASTA scores: opt: 1300, E(): 2.2e-74, (43.3% identity in 693 aa overlap); etc. Belongs to the polyphosphate kinase family. P9WHV9 No intermediary metabolism and respiration P65768 P9WHV9 GO:0006799,GO:0008976,GO:0009358,GO:0005524 2.7.4.1 P9WHV9 NC_000962.3 Mycobrowser_v5 CDS 3342165 3343118 . + 0 Rv2985 mutT1 Function unknown; hydrolytic enzyme. Possibly involved in removal of damaged nucleotide. Possible hydrolase MutT1 Rv2985, (MTCY349.02c), len: 317 aa. Possible mutT1, long MutT protein (hydrolase) (see citation below), highly similar to O33126|MLCB637.35 hypothetical 34.5 KDA protein from Mycobacterium leprae (312 aa), FASTA scores: opt: 1514, E(): 5.1e-91, (71.85% identity in 316 aa overlap); and Q9CBR8|ML1682 hypothetical protein from Mycobacterium leprae (311 aa), FASTA scores: opt: 1510, E(): 9.2e-91, (71.5% identity in 316 aa overlap). Also similar to Q50195|L222-ORF6|ML2698 hypothetical protein from Mycobacterium leprae (251 aa), FASTA scores: opt: 231, E(): 1.1e-07, (36.7% identity in 128 aa overlap). Also similar to shorter mutt proteins and related hypothetical protein e.g. Q9EUS6 hypothetical 16.6 KDA protein from Streptomyces griseus subsp. griseus (152 aa), FASTA scores: opt: 380, E(): 1.7e-17, (50.75% identity in 130 aa overlap); Q9KZV8|SCD84.10C putative mutt-like protein from Streptomyces coelicolor (142 aa), FASTA scores: opt: 376, E(): 2.9e-17, (46.1% identity in 128 aa overlap); P96590|mutt mutt protein from Bacillus subtilis (149 aa), FASTA scores: opt: 180, E(): 0.00017, (35.25% identity in 122 aa overlap); etc. Also similar to O05437 hypothetical 27.1 KDA protein from Mycobacterium tuberculosis (248 aa), FASTA scores: opt: 224, E(): 3.2e-07, (34.03% identity in 144 aa overlap). Contains PS00893 mutT domain signature. Seems to belong to the mutt/NUDIX family protein. P9WIY3 No information pathways P95110 P9WIY3 GO:0006260,GO:0016787,GO:0046872,GO:0006281 3.6.1.- P9WIY3 NC_000962.3 Mycobrowser_v5 CDS 3346147 3346848 . + 0 Rv2989 Rv2989 Involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv2989, (MTV012.03), len: 233 aa. Probable transcriptional regulator (ala-rich protein), highly similar to O86533|SC1C2.33c putative transcriptional regulator from Streptomyces coelicolor (238 aa), FASTA scores: opt: 711, E(): 2.3e-38, (53.05% identity in 230 aa overlap); and similar to others e.g. Q9KND6 putative transcriptional regulator from Vibrio cholerae (244 aa), FASTA scores: opt: 232, E(): 1.2e-07, (29.75% identity in 232 aa overlap); Q9R9U0|SRPS efflux pump regulator from Pseudomonas putida (259 aa), FASTA scores: opt: 224, E(): 4.1e-07, (28.35% identity in 247 aa overlap); etc. Also similar to proteins from Mycobacterium tuberculosis e.g. O06806|Rv1773c|MTCY28.39 hypothetical 26.6 KDA protein (248 aa), FASTA scores: opt: 239, E(): 4.4e-08, (29.85% identity in 231 aa overlap); P71977|RV1719|MTCY04C12.04 hypothetical 27.9 KDA protein (259 aa), FASTA scores: opt: 215, E(): 1.6e-06, (31.85% identity in 223 aa overlap); etc. Equivalent to AAK47396 from Mycobacterium tuberculosis strain CDC1551 (267 aa) but shorter 34 aa. Contains possible helix-turn-helix motif at aa 25-46 (Score 1005, +2.61 SD). O53238 No regulatory proteins O53238 O53238 GO:0006355,GO:0006350,GO:0003677 O53238 NC_000962.3 Mycobrowser_v5 CDS 3351269 3352606 . + 0 Rv2994 Rv2994 Unknown; COULB be involved in efflux system (possibly drug). Probable conserved integral membrane protein Rv2994, (MTV012.08), len: 445 aa. Probable conserved integral membrane protein, member of major facilitator superfamily (MFS) possibly involved in transport of drug. C-terminal part highly similar to O33118|MLCB637.27c hypothetical 14.7 KDA protein (probable pseudogene product) from Mycobacterium leprae (134 aa), FASTA scores: opt: 483, E(): 2.7e-21, (60.9% identity in 138 aa overlap). Also similar to various transporters e.g. Q9I5C8|PA0811 probable MFS transporter from Pseudomonas aeruginosa (415 aa), FASTA scores: opt: 289, E(): 1.3e-09, (26.05% identity in 399 aa overlap); O30210|AF0025 cyanate transport protein from Archaeoglobus fulgidus (393 aa), FASTA scores: opt: 281, E(): 3.7e-09, (24.05% identity in 399 aa overlap); Q9RI35|SCJ12.25C putative nitrate/nitrite transporter from Streptomyces coelicolor (412 aa), FASTA scores: opt: 264, E(): 3.8e-08, (24.95% identity in 409 aa overlap); Q9A5N5|CC2412 major facilitator family transporter from Caulobacter crescentus (405 aa), FASTA scores: opt: 263, E(): 4.3e-08, (27.55% identity in 399 aa overlap); etc. First start taken; similarity to P21191|NORA_STAAU quinolone resistance protein from Staphylococcus aureus (388 aa) suggests alternative start at 7319 but then no positively charged aa before first transmembrane segment. P9WJW7 No cell wall and cell processes Q7ARU7 P9WJW7 GO:0005886,GO:0055085,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 3352458 3353468 . - 0 Rv2995c leuB Involved in leucine biosynthesis (at the third step) [catalytic activity: 3-carboxy-2-hydroxy-4-methylpentanoate + NAD(+) = 3-carboxy-4-methyl-2-oxopentanoate + NADH (the product decarboxylates to 4-methyl-2-oxopentanoate)]. Probable 3-isopropylmalate dehydrogenase LeuB (beta-IPM dehydrogenase) (IMDH) (3-IPM-DH) Rv2995c, (MTV012.09), len: 336 aa. Probable leuB, 3-isopropylmalate dehydrogenase, identical except a single bp to P94929|LEU3_MYCBO 3-isopropylmalate dehydrogenase from Mycobacterium bovis (336 aa) (see citation below), FASTA scores: opt: 2168, E(): 5.1e-132, (99.7% identity in 336 aa overlap); and equivalent to O33117|LEU3_MYCLE 3-isopropylmalate dehydrogenase from Mycobacterium leprae (336 aa), FASTA scores: opt: 1864, E(): 1.8e-112, (83.95% identity in 336 aa overlap). Also highly similar to others e.g. P94631|LEU3_CORGL from Corynebacterium glutamicum (340 aa), FASTA scores: opt: 1526, E(): 1e-90, (69.9% identity in 339 aa overlap); O86504 from Streptomyces coelicolor (347 aa), FASTA scores: opt: 1470, E(): 4.2e-87, (67.85% identity in 339 aa overlap); Q9UZ05|PAB2424 from Pyrococcus abyssi (354 aa), FASTA scores: opt: 998, E(): 1e-56, (50.0% identity in 322 aa overlap); etc. Note that also shows high similarity with many tartrate dehydrogenases. Belongs to the isocitrate and isopropylmalate dehydrogenases family. P9WKK9 No intermediary metabolism and respiration 1W0D,2G4O P95313 P9WKK9 GO:0051287,GO:0005737,GO:0009098,GO:0000287,GO:0055114,GO:0003862 1.1.1.85 P9WKK9 NC_000962.3 Mycobrowser_v5 CDS 3357602 3358567 . + 0 Rv2999 lppY Unknown Probable conserved lipoprotein LppY Rv2999, (MTV012.13), len: 321 aa. Probable lppY, conserved lipoprotein, highly similar to O07774|LPQO|Rv0604|MTCY19H5.18c putative lipoprotein from Mycobacterium tuberculosis (316 aa), FASTA scores: opt: 1153, E(): 5e-62, (53.2% identity in 312 aa overlap); and showing similarity with AAK80743|CAC2799 uncharacterized conserved protein similar to LPPY/LPQO of Mycobacterium tuberculosis from Clostridium acetobutylicum (152 aa), FASTA scores: opt: 165, E(): 0.0077, (26.08% identity in 138 aa overlap); and Q9F2T1|SCD65.01c putative lipoprotein (fragment) from Streptomyces coelicolor (146 aa), FASTA scores: opt: 126, E(): 1.6, (% identity in aa overlap). Equivalent to AAK47407 from Mycobacterium tuberculosis strain CDC1551 (329 aa) but shorter 8 aa. Contains probable N-terminal signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O53246 No cell wall and cell processes O53246 O53246 O53246 NC_000962.3 Mycobrowser_v5 CDS 3360624 3361130 . - 0 Rv3002c ilvN Involved in valine and isoleucine biosynthesis (at the first step) [catalytic activity: 2-acetolactate + CO(2) = 2-pyruvate]. Probable acetolactate synthase (small subunit) IlvN (acetohydroxy-acid synthase) (AHAS) (ALS) Rv3002c, (MT3082, MTV012.16c), len: 168 aa. Probable ilvN (alternate gene name: ilvH), acetolactate synthase, small subunit, equivalent or highly similar to others e.g. O33113|ILVH_MYCLE|MLCB637.21 from Mycobacterium leprae (169 aa), FASTA scores: opt: 843, E(): 5.1e-47, (83.5% identity in 164 aa overlap); Q59499|ILVH_MYCAV|ILVN from Mycobacterium avium (167 aa), FASTA scores: opt: 798, E(): 3.7e-44, (81.05% identity in 169 aa overlap); Q9Z566|ILVN from Streptomyces coelicolor (174 aa), FASTA scores: opt: 678, E(): 1.7e-36, (64.8% identity in 159 aa overlap); etc. Belongs to the acetolactate synthase small subunit family. P9WKJ3 No intermediary metabolism and respiration P65161 P9WKJ3 GO:0016597,GO:0009082,GO:0003984 2.2.1.6 P9WKJ3 NC_000962.3 Mycobrowser_v5 CDS 3361130 3362986 . - 0 Rv3003c ilvB1 Involved in valine and isoleucine biosynthesis (at the first step) [catalytic activity: 2-acetolactate + CO(2) = 2 pyruvate]. Acetolactate synthase (large subunit) IlvB1 (acetohydroxy-acid synthase) Rv3003c, (MT3083, MTV012.17c), len: 618 aa. ilvB1, acetolactate synthase, large subunit, equivalent or highly similar to others e.g. O33112|ILVB_MYCLE|MLCB637.20|ML1696 from Mycobacterium leprae (625 aa), FASTA scores: opt: 3653, E(): 5.4e-208, (87.1% identity in 627 aa overlap); Q59498|ILVB_MYCAV from Mycobacterium avium (621 aa), FASTA scores: opt: 3473, E(): 2.3e-197, (84.7% identity in 614 aa overlap); P42463|ILVB_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (626 aa), FASTA scores: opt: 2754, E(): 5.9e-155, (65.8% identity in 589 aa overlap); etc. Contains PS00187 Thiamine pyrophosphate enzymes signature. Cofactor: thiamine pyrophosphate, and magnesium (by similarity). Note that previously known as ilvB. P9WG41 No intermediary metabolism and respiration P0A622 P9WG41 GO:0003984,GO:0009082,GO:0000287,GO:0030976,GO:0050660 2.2.1.6 P9WG41 NC_000962.3 Mycobrowser_v5 CDS 3372545 3374620 . - 0 Rv3014c ligA This protein catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA [catalytic activity: NAD(+) + (deoxyribonucleotide)(N) + (deoxyribonucleotide)(M) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(N+M)]. DNA ligase [NAD dependent] LigA (polydeoxyribonucleotide synthase [NAD+]) Rv3014c, (MT3094, MTV012.28c), len: 691 aa. ligA (alternate gene name: lig), DNA ligase NAD-dependent (see citation below), equivalent to O33102|DNLJ_MYCLE|LIGA|LIG|ML1705|MLCB637.10 DNA ligase from Mycobacterium leprae (694 aa), FASTA scores: opt: 3844, E(): 0, (84.7% identity in 687 aa overlap). Also highly similar to many prokaryotic and eukaryotic ligases e.g. Q9Z585|LIGA|SC8D9.06 from Streptomyces coelicolor (735 aa), FASTA scores: opt: 2002, E(): 4e-113, (59.4% identity in 714 aa overlap); P49421|DNLJ_RHOMR|LIGA|LIG from Rhodothermus marinus (712 aa), FASTA scores: opt: 1835, E(): 4.6e-103, (45.55% identity in 685 aa overlap); P15042|DNLJ_ECOLI|LIGA|LIG|DNAL|PDEC|lop|B2411 from Escherichia coli strain K12 (671 aa), FASTA scores: opt: 1696, E(): 1.1e-94, (43.8% identity in 680 aa overlap); etc. Belongs to the NAD-dependent DNA ligase family. P9WNV1 No information pathways 1ZAU P63973 P9WNV1 GO:0003911,GO:0006281,GO:0006260,GO:0005622,GO:0046872,GO:0003677 6.5.1.2 P9WNV1 NC_000962.3 Mycobrowser_v5 CDS 3376490 3376852 . - 0 Rv3017c esxQ Unknown ESAT-6 like protein EsxQ (TB12.9) (ESAT-6 like protein 8) Rv3017c, (MT3097, MTV012.31c), len: 120 aa. EsxQ, ESAT-6 like protein (see citation below), possibly secreted protein, very similar to AAK47433|MT3104 putative secreted ESAT-6 like protein 9 from Mycobacterium tuberculosis strain CDC1551 (96 aa), FASTA scores: opt: 315, E(): 1.2e-14, (65.7% identity in 70 aa overlap); Rv3019c|O53266|MTV012.33c putative secreted ESAT-6 like protein 9 from Mycobacterium tuberculosis (96 aa), FASTA scores: opt: 315, E(): 1.2e-14, (65.7% identity in 70 aa overlap) and Rv0288|O53693|CFP7|MT0301|MTV035.16 10 KDA antigen CFP7 (low molecular weight protein antigen 7) (CFP-7) from Mycobacterium tuberculosis (95 aa), FASTA scores: opt: 303, E(): 7.4e-14, (66.2% identity in 68 aa overlap). An alternative start site exists at 3376801. Belongs to the ESAT6 family. Note previously known as TB12.9. P9WNJ1 No cell wall and cell processes P9WNJ1 P9WNJ1 NC_000962.3 Mycobrowser_v5 CDS 3387075 3388031 . - 0 Rv3028c fixB The electron transfer flavoprotein serves as a specific electron acceptor for other dehydrogenases. It transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Probable electron transfer flavoprotein (alpha-subunit) FixB (alpha-ETF) (electron transfer flavoprotein large subunit) (ETFLS) Rv3028c, (MTV012.43c), len: 318 aa. Probable fixB (alternate gene name: etfA), electron transfer flavoprotein (alpha subunit) for various dehydrogenases. Equivalent to O33096|ETFA_MYCLE|FIXB|ML1711|MLCB637.04 electron transfer flavoprotein from Mycobacterium leprae (318 aa), FASTA scores: opt: 1788, E(): 1.1e-87, (89.3% identity in 318 aa overlap). Also highly similar to many e.g. Q9K418|SCG22.27c from Streptomyces coelicolor (320 aa), FASTA scores: opt: 1161, E(): 1.6e-54, (59.45% identity in 323 aa overlap); AAK08137|etfa from Rhodobacter sphaeroides (308 aa), FASTA scores: opt: 792, E(): 5.1e-35, (45.95% identity in 309 aa overlap); P38974|ETFA_PARDE electron transfer flavoprotein from Paracoccus denitrificans (307 aa), FASTA scores: opt: 789, E(): 7.4e-35, (45.95% identity in 309 aa overlap); etc. Belongs to the Etf alpha-subunit / FixB family. P9WNG9 No intermediary metabolism and respiration O53275 P9WNG9 GO:0009055,GO:0022900,GO:0006810,GO:0050660 P9WNG9 NC_000962.3 Mycobrowser_v5 CDS 3389922 3391502 . + 0 Rv3031 Rv3031 Function unknown Conserved protein Rv3031, (MTV012.46), len: 526 aa. Conserved protein, equivalent to Q9CBR4|ML1714 hypothetical protein from Mycobacterium leprae (522 aa), FASTA scores: opt: 3167, E(): 4.4e-190, (86.15% identity in 526 aa overlap); and highly similar to truncated O33093|MLCB637.01c hypothetical 37.2 KDA protein (fragment) from Mycobacterium leprae (338 aa), FASTA scores: opt: 2041, E(): 5.7e-120, (84.8% identity in 342 aa overlap). Also some similarity to hypothetical proteins Q9V0M7|PAB1857 from Pyrococcus abyssi (602 aa), FASTA scores: opt: 477, E(): 3.5e-22, (31.2% identity in 556 aa overlap); and Synechocystis P74630|D90916|SLL0735 from Synechocystis sp. strain PCC 6803 (529 aa), FASTA scores: opt: 282, E(): 4.7e-10, (28.6% identity in 560 aa overlap). P9WQ27 No conserved hypotheticals O53278 P9WQ27 GO:0003824,GO:0005975 P9WQ27 NC_000962.3 Mycobrowser_v5 CDS 3399419 3400183 . - 0 Rv3039c echA17 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Probable enoyl-CoA hydratase EchA17 (crotonase) (unsatured acyl-CoA hydratase) (enoyl hydrase) Rv3039c, (MTV012.54c), len: 254 aa. Probable echA17, Enoyl-CoA Hydratase/Isomerase Superfamily member (crotonase). Similar to many e.g. Q9L1E6|SC3D11.16 putative enoyl-CoA hydratase from Streptomyces coelicolor (255 aa), FASTA scores: opt: 625, E(): 1.5e-30, (45.55% identity in 224 aa overlap); O07137||ECH8_MYCLE|ML2402|MLCB1306.05c probable enoyl-CoA hydratase ECHA8 from Mycobacterium leprae (257 aa), FASTA scores: opt: 448, E(): 6.4e-20, (35.3% identity in 235 aa overlap), P97087|CRT crotonase / enoyl-CoA hydratase from Clostridium thermosaccharolyticum (Thermoanaerobacterium thermosaccharolyticum) (259 aa), FASTA scores: opt: 420, E(): 3.1e-18, (31.2% identity in 234 aa overlap). Also similar to Mycobacterium tuberculosis AAK45356|O53418|Rv1070c|ECHA8|MT1100|MTV017.23c probable enoyl-CoA hydratase ECHA8 (257 aa), FASTA scores: opt: 450, E(): 4.9e-20, (36.4% identity in 226 aa overlap). Belongs to the enoyl-CoA hydratase/isomerase family. P9WNN3 No lipid metabolism O53286 P9WNN3 GO:0006631,GO:0004300 4.2.1.17 P9WNN3 NC_000962.3 Mycobrowser_v5 CDS 3400192 3401058 . - 0 Rv3040c Rv3040c Function unknown Conserved protein Rv3040c, (MTV012.55c), len: 288 aa. Conserved protein, highly similar to Q9XA40|SCH17.07c hypothetical protein from Streptomyces coelicolor (312 aa), FASTA scores: opt: 648, E(): 5.2e-34, (50.0% identity in 260 aa overlap). Also similar to Q9F7R7 predicted mutt superfamily hydrolase from uncultured proteobacterium EBAC31A08 (264 aa), FASTA scores: opt: 295, E(): 1.3e-11, (27.2% identity in 257 aa overlap); AAK24293|CC2322 hypothetical protein from Caulobacter crescentus (254 aa), blast scores: 185 (32% identity) and 131 (37% identity), etc. O53287 No conserved hypotheticals O53287 O53287 GO:0016787 O53287 NC_000962.3 Mycobrowser_v5 CDS 3405136 3406215 . + 0 Rv3044 fecB May be involved in active transport of FeIII-decitrate across the membrane (import). Probable FEIII-dicitrate-binding periplasmic lipoprotein FecB Rv3044, (MTV012.59), len: 359 aa. Probable fecB, FeIII dicitrate-binding periplasmic lipoprotein (see citation below), equivalent to Q9CBQ4|FECB|ML1729 putative FEIII-dicitrate transporter lipoprotein from Mycobacterium leprae (364 aa), FASTA scores: opt: 1816, E(): 1.1e-96, (75.65% identity in 357 aa overlap); and Q9LA57|FECB from Mycobacterium avium (364 aa), FASTA scores: opt: 1769, E(): 5.1e-94. Similar to many periplasmic FeIII-dicitrate transporters e.g. P72593|FECB|SLR1319 from Synechocystis sp. strain PCC 6803 (315 aa), FASTA scores: opt: 459, E(): 3.6e-19, (31.35% identity in 303 aa overlap); and P72611|FECB|SLR1492 from Synechocystis sp. strain PCC 6803. N-terminus longer (approximately 30 aa) to AAK47459 from Mycobacterium tuberculosis strain CDC1551 (327 aa). Has signal peptide and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O53291 No cell wall and cell processes O53291 O53291 GO:0005381,GO:0006827 O53291 NC_000962.3 Mycobrowser_v5 CDS 3414719 3414958 . - 0 Rv3053c nrdH Involved in electron transfer system for ribonucleotide reductase system NRDEF. Probable glutaredoxin electron transport component of NRDEF (glutaredoxin-like protein) NrdH Rv3053c, (MTCY22D7.29), len: 79 aa. Probable nrdH, glutaredoxin-like protein, equivalent to Q9CBP8|NRDH|ML1736 from Mycobacterium leprae (80 aa), FASTA scores: opt: 478, E(): 2.7e-27, (91.15% identity in 79 aa overlap), and similar to many glutaredoxin-like proteins e.g. Q9XD65|NRDH from Corynebacterium glutamicum (Brevibacterium flavum) (77 aa), FASTA scores: opt: 382, E(): 1.5e-20, (72.35% identity in 76 aa overlap); and Q56108|NRDH_SALTY from Salmonella typhimurium (81 aa), FASTA scores: opt: 243, E(): 9.9e-11, (45.85% identity in 72 aa overlap). Belongs to the glutaredoxin family. I6YB06 No information pathways P95106 I6YB06 GO:0009055,GO:0015035,GO:0045454 I6YB06 NC_000962.3 Mycobrowser_v5 CDS 3416081 3416695 . + 0 Rv3055 Rv3055 Involved in transcriptional mechanism. Possible transcriptional regulatory protein (probably TetR-family) Rv3055, (MTCY22D7.26c), len: 204 aa. Possible transcriptional regulatory protein, similar to Q9RD23|SCM1.20c putative TetR-family transcriptional regulator from Streptomyces coelicolor (234 aa), FASTA scores: opt: 471, E(): 4.6e-23, (44.9% identity in 187 aa overlap); and with low similarity to other e.g. Q9ADK8|2SCK31.12 putative TetR-family transcriptional regulator from Streptomyces coelicolor (198 aa), FASTA scores: opt: 208, 2.5e-06, (32.9% identity in 155 aa overlap); Q9ADD9|SCBAC20F6.11c putative TetR-family transcriptional from Streptomyces coelicolor (199 aa), FASTA scores: opt: 182, E(): 0.00012, (31.0% identity in 184 aa overlap). Contains potential helix-turn-helix motif from aa 48 to 69 (+3.42 SD). so may belong to the TetR/AcrR family of transcriptional regulators. P95103 No regulatory proteins P95103 P95103 GO:0006350,GO:0003700,GO:0006355 P95103 NC_000962.3 Mycobrowser_v5 CDS 3417799 3418662 . - 0 Rv3057c Rv3057c Unknown, but similar to various oxidoreductases and enzymes involved in polyketides synthesis Probable short chain alcohol dehydrogenase/reductase Rv3057c, (MTCY22D7.24), len: 287 aa. Probable oxidoreductase, probably short-chain alcohol dehydrogenase/reductase. Equivalent to Q9CBP7|ML1740 possible short chain dehydrogenases/reductase from Mycobacterium leprae (312 aa), FASTA scores: opt: 1563, E(): 6e-89, (81.8% identity in 280 aa overlap). Also similar to many oxidoreductases e.g. Q9ZBX8|SCD78.21c putative oxidoreductase from Streptomyces coelicolor (585 aa), FASTA scores: opt: 541, E(): 6.7e-26, (37.25% identity in 263 aa overlap); AAK47506|MT3170 oxidoreductase, short-chain dehydrogenase/reductase family from Mycobacterium tuberculosis strain CDC1551 (276 aa), FASTA scores: opt: 521, E(): 6.1e-25, (36.25% identity in 276 aa overlap); AAK45541|MT1283 oxidoreductase, short-chain dehydrogenase/reductase family from Mycobacterium tuberculosis strain CDC1551 (276 aa), FASTA scores: opt: 471, E(): 7.2e-22, (32.4% identity in 281 aa overlap). Also similar to O50460|Rv1245c|MTV006.17C dehydrogenase (276 aa). Contains short-chain alcohol dehydrogenase family signature (PS00061). May belong to the short-chain dehydrogenases/reductases (SDR) family. I6YB11 No intermediary metabolism and respiration P95101 I6YB11 GO:0055114,GO:0016491 1.1.-.- I6YB11 NC_000962.3 Mycobrowser_v5 CDS 3418726 3419376 . - 0 Rv3058c Rv3058c Involved in transcriptional mechanism. Possible transcriptional regulatory protein (probably TetR-family) Rv3058c, (MTCY22D7.23), len: 216 aa. Possible transcriptional regulatory protein, TetR-family, showing reasonable similarity to others e.g. AAK48337|MT3970 from Mycobacterium tuberculosis strain CDC1551 (216 aa), FASTA scores: opt: 261, E(): 2.8e-10, (31.7% identity in 221 aa overlap); Q49962|ML1070|U1756B from Mycobacterium leprae (217 aa), FASTA scores: opt: 234, E(): 1.8e-08, (27.2% identity in 195 aa overlap); Q9CDD3|ML0064 from Mycobacterium leprae (214 aa), FASTA scores: opt: 199, E(): 3.6e-06, (25.65% identity in 195 aa overlap); O66121|CPRS from Streptomyces coelicolor (215 aa), FASTA scores: opt: 183, E(): 4.2e-05, (26.0% identity in 196 aa overlap). Equivalent to AAK47476|MT3144 from Mycobacterium tuberculosis strain CDC1551 (237 aa) but N-terminus shorter 21 residues. Start was predicted by TBParse but alternatives (ATG) are possible. Could belong to the TetR/AcrR family of transcriptional regulators. P95100 No regulatory proteins P95100 P95100 GO:0006350,GO:0003700,GO:0006355 P95100 NC_000962.3 Mycobrowser_v5 CDS 3419492 3420970 . + 0 Rv3059 cyp136 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Probable cytochrome P450 136 Cyp136 Rv3059, (MTCY22D7.22c), len: 492 aa. Probable cyp136, cytochrome P450 136, similar to other cytochrome P450-dependent oxidases e.g. Q59990|CYP120|CYP|SLR0574 putative cytochrome P450 120 from Synechocystis sp. strain PCC 6803 (444 aa), FASTA scores: opt: 579, E(): 1.5e-29, (27.3% identity in 443 aa overlap); Q64654|CYP51|CP51_RAT cytochrome P450 51 (lanosterol 14-alpha demethylase) from Rattus norvegicus (Rat) (503 aa), FASTA scores: opt: 549, E(): 1.4e-27, (26.2% identity in 458 aa overlap); Q9JIY3|CYP51 lanosterol 14-alpha-demethylase from Mus musculus (Mouse) (486 aa), FASTA scores: opt: 546, E(): 2.1e-27, (25.75% identity in 458 aa overlap). Contains cytochrome P450 cysteine heme-iron ligand signature (PS00086). Belongs to the cytochrome P450 family. P9WPM7 No intermediary metabolism and respiration P95099 P9WPM7 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPM7 NC_000962.3 Mycobrowser_v5 CDS 3423262 3425427 . - 0 Rv3061c fadE22 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE22 Rv3061c, (MTCY22D7.20), len: 721 aa. Probable fadE22, Acyl-CoA Dehydrogenase, similar to many e.g. AAK44503|MT0284 from Mycobacterium tuberculosis strain CDC1551 (731 aa), FASTA scores: opt: 1804, E(): 1.1e-101, (43.45% identity in 743 aa overlap); AAK48037|MT3678 from Mycobacterium tuberculosis strain CDC1551 (711 aa), FASTA scores: opt: 1630, E(): 3.9e-91, (42.55% identity in 733 aa overlap); and extensive similarity in C-terminal part to many acyl-CoA dehydrogenases e.g. Q9A5G9|CC2478 from Caulobacter crescentus (407 aa), FASTA scores: opt: 767, E(): 4.8e-39, (36.7% identity in 376 aa overlap). Also similar to many hypothetical proteins. Could belong to the acyl-CoA dehydrogenases family. I6X654 No lipid metabolism P95097 I6X654 GO:0003995,GO:0055114,GO:0050660 1.3.99.- I6X654 NC_000962.3 Mycobrowser_v5 CDS 3431979 3433622 . - 0 Rv3068c pgmA This enzyme participates in both the breakdown and synthesis of glucose [catalytic activity: alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate]. Probable phosphoglucomutase PgmA (glucose phosphomutase) (PGM) Rv3068c, (MTCY22D7.13), len: 547 aa. Probable pgmA, phosphoglucomutase, highly similar to other phosphoglucomutases e.g. Q9L117|PGM from Streptomyces coelicolor (546 aa), FASTA scores: opt: 2569, E(): 2.8e-149, (71.4% identity in 545 aa overlap); Q9ABY5|CC0085 from Caulobacter crescentus (545 aa), FASTA scores: opt: 2465, E(): 6.2e-143, (70.4% identity in 541 aa overlap); P38569|PGMU_ACEXY|CELB from Acetobacter xylinum (555 aa), FASTA scores: opt: 2206, E(): 4e-127, (62.25% identity in 543 aa overlap); P74643|PGM|SLL0726 from Synechocystis sp. strain PCC 6803 (567 aa), FASTA scores: opt: 2168, E(): 8.5e-125, (60.0% identity in 550 aa overlap); P36938|PGMU_ECOLI|PGM|B0688 from Escherichia coli (546 aa), FASTA scores: opt: 2111, E(): 2.5e-121, (58.2% identity in 550 aa overlap). Also similar to other phosphomannomutases. Has phosphoglucomutase and phosphomannomutase signature (PS00710) and ATP/GTP-binding site motif A (P-loop) (PS00017). Belongs to the phosphohexose mutases family. I6Y2G3 No intermediary metabolism and respiration P95090 I6Y2G3 GO:0000287,GO:0004614,GO:0005975 5.4.2.2 I6Y2G3 NC_000962.3 Mycobrowser_v5 CDS 3435798 3436322 . - 0 Rv3072c Rv3072c Function unknown Conserved hypothetical protein Rv3072c, (MTCY22D7.09), len: 174 aa. Hypothetical protein, similar in part to O87779 hypothetical 18.1 KDA protein (fragment) from Mycobacterium paratuberculosis (166 aa), FASTA scores: opt: 238, E(): 2.5e-08, (42.6% identity in 108 aa overlap); Q9AH10 putative F420-dependent dehydrogenase from Rhodococcus erythropolis (295 aa), FASTA scores: opt: 228, E(): 1.7e-07, (34.25% identity in 111 aa overlap); P71557|Y953_MYCTU|Rv0953c|MTCY10D7.21 possible oxidoreductase from Mycobacterium tuberculosis strain H37Rv (304 aa), FASTA scores: opt: 208, E(): 3.2e-06, (38.9% identity in 108 aa overlap); etc. N-terminal region similar to several proteins from Mycobacterium tuberculosis. P95086 No conserved hypotheticals P95086 P95086 GO:0016491,GO:0055114 P95086 NC_000962.3 Mycobrowser_v5 CDS 3438050 3438973 . - 0 Rv3075c Rv3075c Function unknown Conserved protein Rv3075c, (MTCY22D7.06), len: 307 aa. Conserved protein, with some similarity to Q9I562|PA0883 probable acyl-CoA lyase beta chain from Pseudomonas aeruginosa (275 aa), FASTA scores: opt: 408, E(): 9.2e-19, (35.15% identity in 273 aa overlap); Q9S2U9|SC4G6.02 putative citrate lyase beta chain from Streptomyces coelicolor (274 aa), FASTA scores: opt: 384, E(): 3.1e-17, (34.7% identity in 265 aa overlap); O06162|cite|Rv2498c|MTCY07A7.04c from Mycobacterium tuberculosis (273 aa), FASTA scores: opt: 349, E(): 5.1e-15, (35.2% identity in 264 aa overlap); etc. Several initiation codons possible, first one chosen. I6YF40 No conserved hypotheticals P95083 I6YF40 GO:0008815,GO:0009346,GO:0046872,GO:0006725 I6YF40 NC_000962.3 Mycobrowser_v5 CDS 3439541 3441352 . + 0 Rv3077 Rv3077 Function unknown; probably involved in cellular metabolism. Possible hydrolase Rv3077, (MTCY22D7.04c), len: 603 aa. Possible hydrolase, with some similarity to variety of hydrolases (aryl- and steryl sulfatases principaly) e.g. Q45087|PEHA phosphonate monoester hydrolase from Burkholderia caryophylli (514 aa), FASTA scores: opt: 239, E(): 7.2e-07, (23.95% identity in 413 aa overlap); Q9I1E5|PA2333 probable sulfatase from Pseudomonas aeruginosa (538 aa), FASTA scores: opt: 231, E(): 2.3e-06, (28.1% identity in 516 aa overlap); P31447|YIDJ_ECOLI|B3678 putative sulfatase from Escherichia coli (497 aa), FASTA scores: opt: 222, E(): 7.4e-06, (27.7% identity in 390 aa overlap); etc. Note that previously known as atsF. Q6MX15 No intermediary metabolism and respiration Q6MX15 Q6MX15 GO:0008484,GO:0008152 3.1.-.- NC_000962.3 Mycobrowser_v5 CDS 3441770 3442597 . - 0 Rv3079c Rv3079c Function unknown Conserved protein Rv3079c, (MTCY22D7.02), len: 275 aa. Conserved protein, similar to other hypothetical mycobacterium proteins e.g. P71557|Y953_MYCTU|Rv0953c|MTCY10D7.21 possible oxidoreductase from Mycobacterium tuberculosis strain H37Rv (282 aa), FASTA scores: opt: 668, E(): 2.4e-34, (40.55% identity in 281 aa overlap); O06216|Rv2161c|MTCY270.07 from Mycobacterium tuberculosis strain H37Rv (288 aa), FASTA scores: opt: 595, E(): 8.5e-30, (40.9% identity in 274 aa overlap); O87779 from Mycobacterium paratuberculosis (166 aa), FASTA scores: opt: 464, E(): 7.2e-22, (41.55% identity in 166 aa overlap); etc. Also some similarity to other proteins e.g. Q9AH10 putative F420-dependent dehydrogenase from Rhodococcus erythropolis (295 aa), FASTA scores: opt: 401, E(): 9.6e-18, (30.2% identity in 288 aa overlap); Q9AE04|RIF17 RIF17 protein from Amycolatopsis mediterranei (356 aa), FASTA scores: opt: 298, E(): 2.8e-11, (35.0% identity in 203 aa overlap); AAK48081|MT3720 luciferase-related protein from Mycobacterium tuberculosis strain CDC1551 (395 aa), FASTA scores: opt: 223, E(): 1.4e-06, (29.4% identity in 211 aa overlap). I6XG43 No conserved hypotheticals P95079 I6XG43 GO:0016491,GO:0055114 I6XG43 NC_000962.3 Mycobrowser_v5 CDS 3442656 3445988 . - 0 Rv3080c pknK Involved in signal transduction (via phosphorylation). Involved in transcriptional regulatory mechanism and in the regulation of secondary metabolites [catalytic activity: ATP + a protein = ADP + a phosphoprotein]. Serine/threonine-protein kinase transcriptional regulatory protein PknK (protein kinase K) (STPK K) Rv3080c, (MTV013.01c-MTCY22D7.01), len: 1110 aa. pknK, serine/threonine protein kinase involved in transcriptional regulatory function (see citation below). Similar but shorter in N-terminus (approximately 300 residues) to others e.g. Q48411|ACOK transcriptional regulatory protein of aco ABCD operon from Klebsiella pneumoniae (921 aa), FASTA scores: opt: 886, E(): 7.6e-37, (27.75% identity in 829 aa overlap); Q9HX92|PA3921 probable transcriptional regulator from Pseudomonas aeruginosa (belongs to the LuxR/UhpA family of transcriptional regulators) (906 aa), FASTA scores: opt: 760, E(): 1.5e-30, (29.55% identity in 822 aa overlap); Q9I2X9|PA1760 probable transcriptional regulator from Pseudomonas aeruginosa (belongs to the LuxR/UhpA family of transcriptional regulators) (907 aa), FASTA scores: opt: 696, E(): 2.3e-27, (25.85% identity in 685 aa overlap); P06993|malt (alias BAB37683|ECS4260 and AAG58520|malt) positive regulator of MAL regulon from Escherichia coli strain O157:H7 (901 aa), FASTA scores: opt: 660, E(): 1.4e-25, (29.25% identity in 530 aa overlap); Q9KNF3|VCA0011 malt regulatory protein from Vibrio cholerae (belongs to the LuxR/UhpA family of transcriptional regulators) (921 aa), FASTA scores: opt: 626, E(): 7.2e-24, (25.8% identity in 659 aa overlap); etc. N-terminal region similar to N-terminus of serine/threonine kinases e.g. Q9KK90|PKMA serine/threonine kinase (similar to the Ser/Thr family of protein kinases) from Amycolatopsis mediterranei (589 aa), FASTA scores: opt: 545, E(): 5.7e-20, (34.45% identity in 334 aa overlap); Q9RPT5|AMK serine/threonine protein kinase homolog (similar to the Ser/Thr family of protein kinases) from Amycolatopsis mediterranei (606 aa), FASTA scores: opt: 537, E(): 1.5e-19, (35.55% identity in 346 aa overlap); Q9L0I0|PKAD protein serine/threonine kinase from Streptomyces coelicolor (599 aa), FASTA scores: opt: 520, E(): 1e-18, (36.1% identity in 324 aa overlap); etc. N-terminal part also similar to O53510|PKNL_MYCTU|Rv2176|MT2232|MTV021.09 probable serine/threonine-protein kinase from Mycobacterium tuberculosis strain H37Rv (399 aa), FASTA scores: opt: 511, E(): 2.1e-18, (35.15% identity in 313 aa overlap). Contains PS00107 Protein kinases ATP-binding region signature and PS00017 ATP/GTP-binding site motif A (P-loop). Contains Hank's kinase subdomain. First part of the protein seems belong to the Ser/Thr family of protein kinases, and second parts seems belongs to the LuxR/UhpA family of transcriptional regulators. P9WI65 No regulatory proteins P95078 P9WI65 GO:0006468,GO:0004674,GO:0005524 2.7.11.1 P9WI65 NC_000962.3 Mycobrowser_v5 CDS 3450920 3451750 . + 0 Rv3085 Rv3085 Function unknown; supposedly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv3085, (MTV013.06), len: 276 aa. Probable short-chain dehydrogenase/reductase, similar to various oxidoreductases in the short chain dehydrogenases/reductases family e.g. Q9CC98|ML1094 short chain alcohol dehydrogenase from Mycobacterium leprae (277 aa), FASTA scores: opt: 1059, E(): 4.8e-56, (61.65% identity in 266 aa overlap); Q9I3H6|PA1537 probable short-chain dehydrogenase from Pseudomonas aeruginos (295 aa), FASTA scores: opt: 858, E(): 4.7e-44, (48.4% identity in 285 aa overlap); Q9CBP7|ML1740 possible short chain reductase from Mycobacterium leprae (312 aa), FASTA scores: opt: 500, E(): 1e-22, (36.6% identity in 257 aa overlap); etc. Also similar to mycobacterium proteins O50460|Rv1245c|MTV006.17c dehydrogenase similar to the short-chain dehydrogenases/reductases family (276 aa), FASTA scores: opt: 1200, E(): 1.9e-64, (65.2% identity in 273 aa overlap); and P95101|Rv3057c|MTCY22D7.24 hypothetical dehydrogenase (287 aa). Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. P9WGP9 No intermediary metabolism and respiration O53302 P9WGP9 GO:0055114,GO:0016491 1.-.-.- P9WGP9 NC_000962.3 Mycobrowser_v5 CDS 3455761 3457272 . + 0 Rv3089 fadD13 Function unknown, but involved in lipid degradation. Probable chain -fatty-acid-CoA ligase FadD13 (fatty-acyl-CoA synthetase) Rv3089, (MTV013.10), len: 503 aa. Probable fadD13, Acyl-CoA Synthetase, similar to many e.g. MTCI28.06, MTCY08D5.09, MTCY06G11.08 from Mycobacterium tuberculosis strain H37Rv; and to Q9F7P5 predicted acid--CoA ligase FADD13 from uncultured proteobacterium EBAC31A08 (504 aa), FASTA scores: opt: 1126, E(): 2.4e-62, (38.85% identity in 502 aa overlap); Q9EY88|FCS feruloyl-CoA synthetase from Amycolatopsis sp. strain HR167 (491 aa), FASTA scores: opt: 1073, E(): 4.5e-59, (38.5% identity in 504 aa overlap); BAB49118|MLR1843 probable acid-CoA ligase from Rhizobium loti (Mesorhizobium loti) (495 aa), FASTA scores: opt: 937, E(): 1.2e-50, (36.2% identity in 503 aa overlap); Q9KZC1|SC6F7.21 probable long-chain-fatty-acid-CoA ligase from Streptomyces coelicolor (511 aa), FASTA scores: opt: 899, E(): 2.8e-48, (36.1% identity in 510 aa overlap); Q9A5P7|CC2400 putative acid-CoA ligase from Caulobacter crescentus (496 aa), FASTA scores: opt: 874, E(): 9.8e-47, (35.1% identity in 507 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature and PS00061 Short-chain alcohol dehydrogenase family signature. P9WQ37 No lipid metabolism O53306 P9WQ37 GO:0008152,GO:0016874 6.2.1.- P9WQ37 NC_000962.3 Mycobrowser_v5 CDS 3459116 3460807 . + 0 Rv3091 Rv3091 Function unknown Conserved protein Rv3091, (MTCY164.02), len: 563 aa. Conserved protein, similar in part to O60859 neuropathy target esterase from Homo sapiens (Human) (1327 aa), FASTA scores: opt: 177, E(): 0.0062, (30.65% identity in 173 aa overlap); and Q9I385|PA1640 hypothetical protein from Pseudomonas aeruginosa (345 aa), FASTA scores: opt: 152, E(): 0.069, (27.8% identity in 180 aa overlap). I6YB49 No conserved hypotheticals O05770 I6YB49 GO:0006629 NC_000962.3 Mycobrowser_v5 CDS 3460814 3461734 . - 0 Rv3092c Rv3092c Unknown Probable conserved integral membrane protein Rv3092c, (MTCY164.03c), len: 306 aa. Probable conserved integral membrane protein, highly similar to Q9RUT5|DR1297 conserved hypothetical protein from Deinococcus radiodurans (311 aa), FASTA scores: opt: 941, E(): 9.8e-51, (55.65% identity in 309 aa overlap); Q9A8B8|CC1436 hypothetical protein from Caulobacter crescentus (314 aa), FASTA scores: opt: 791, E(): 1.6e-41, (46.9% identity in 305 aa overlap); and also highly similar to Q9I2N8|PA1857 hypothetical protein from Pseudomonas aeruginosa (307 aa), FASTA scores: opt: 373, E(): 8.1e-16, (40.8% identity in 321 aa overlap); BAB36119|ECS2696 putative methyl-independent mismatch repair protein from Escherichia coli strain O157:H7 (305 aa), FASTA scores: opt: 335, E(): 1.7e-13, (39.75% identity in 307 aa overlap). I6Y2I9 No cell wall and cell processes O05771 I6Y2I9 NC_000962.3 Mycobrowser_v5 CDS 3465778 3467091 . - 0 Rv3097c lipY Generates diacylglycerol and a fatty acid anion from triacylglycerol [catalytic activity: triacylglycerol + H(2)O = diacylglycerol + a fatty acid anion]. PE-PGRS family protein, triacylglycerol lipase LipY (esterase/lipase) (triglyceride lipase) (tributyrase) Rv3097c, (MTCY164.08c), len: 437 aa. LipY, triacylglycerol lipase. Belongs to the hormone-sensitive lipase family (See Deb et al., 2006) and member of the M. tuberculosis PE-family PGRS subfamily of gly-rich proteins (see citation below); N-terminal part similar to N-terminus of M. tuberculosis PE-PGRS family members e.g. Q10637|Y03A_MYCTU hypothetical glycine-rich 49.6 kDa protein (603 aa). Other relatives include MTCY1A11.25c; MTCY21B4.13c; MTCY270.06; MTCY359.33; MTC1A11.04. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6Y2J4 No PE/PPE P77909 I6Y2J4 GO:0004806 3.1.1.3 I6Y2J4 NC_000962.3 Mycobrowser_v5 CDS 3469301 3469783 . - 0 Rv3100c smpB Binds specifically to the SSRA RNA (TMRNA) and is required for stable association of SSRA with ribosomes. Thought to be implicated in the survival of bacterium within macrophages. Probable SSRA-binding protein SmpB Rv3100c, (MTCY164.11c), len: 160 aa. Probable smpB, small protein b related to several bacterial small protein b homologs e.g. O32881|SSRP_MYCLE|ML0671|MLCB1779.19c from Mycobacterium leprae (160 aa), FASTA scores: opt: 914, E(): 1.1e-52, (84.9% identity in 159 aa overlap); Q9L1S9|SMPB from Streptomyces coelicolor (159 aa), FASTA scores: opt: 568, E(): 3.3e-30, (55.15% identity in 145 aa overlap); O32230|SSRP_BACSU from Bacillus subtilis (156 aa), FASTA scores: opt: 511, E(): 1.7e-26, (47.05% identity in 153 aa overlap); etc. Belongs to the SSRP family. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P9WGD3 No virulence, detoxification, adaptation P0A612 P9WGD3 GO:0005737,GO:0006412,GO:0003723 P9WGD3 NC_000962.3 Mycobrowser_v5 CDS 3469786 3470679 . - 0 Rv3101c ftsX Involved in growth (principally during log phase cells). Thought to be involved in active transport of septation component across the membrane. Responsible for the translocation of the substrate across the membrane. Is coded in an operon essential for cell division. Putative cell division protein FtsX (septation component-transport integral membrane protein ABC transporter) Rv3101c, (MTCY164.12c), len: 297 aa. Putative ftsX, cell division protein, septation component transport integral membrane protein ABC transporter (see citations below), equivalent to O32882|FTSX_MYCLE|ML0670|MLCB1779.20c cell division protein from Mycobacterium leprae (297 aa), FASTA scores: opt: 1597, E(): 9.2e-93, (80.8% identity in 297 aa overlap); and similar to others e.g. Q9L1S7|SCE59.27c from Streptomyces coelicolor (305 aa), FASTA scores: opt: 585, E(): 1.9e-29, (34.55% identity in 304 aa overlap); O34876|FTSX_BACSU from Bacillus subtilis (296 aa), FASTA scores: opt: 318, E(): 9.1e-13, (24.65% identity in 300 aa overlap); Q9K6X3|FTSX|BH3601 from Bacillus halodurans (298 aa), FASTA scores: opt: 290, E(): 5.2e-11, (22.75% identity in 299 aa overlap); etc. Belongs to the FTSX family. P9WG19 No cell wall and cell processes P96293 P9WG19 GO:0051301,GO:0016021,GO:0005886,GO:0007049 P9WG19 NC_000962.3 Mycobrowser_v5 CDS 3472768 3473904 . - 0 Rv3105c prfB Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA. Probable peptide chain release factor 2 PrfB (RF-2) Rv3105c, (MTCY164.15c), len: 378 aa. Probable prfB, peptide chain release factor 2, equivalent to O32885|RF2_MYCLE|ML0667|MLCB1779.24c from Mycobacterium leprae, FASTA scores: opt: 2197, E(): 1.8e-126, (90.05% identity in 372 aa overlap); and also similar to other peptide chain release factors e.g. Q9L1S3|PRFB from Streptomyces coelicolor (368 aa), FASTA scores: opt: 1674, E(): 1.2e-94, (69.3% identity in 365 aa overlap); O67695|RF2_AQUAE|PRFB|AQ_1840 from Aquifex aeolicus (373 aa), FASTA scores: opt: 1082, E(): 1.3e-58, (44.45% identity in 369 aa overlap); P28367|RF2_BACSU from B. subtilis (366 aa), FASTA scores: opt: 1030, E(): 1.9e-55, (44.0% identity in 359 aa overlap); etc. Also related to Q10605|MTCY373.19|RF1_MYCTU|Rv1299|MT1338 peptide chain release factor 1 (rf-1) (357 aa), FASTA scores: opt: 646, E(): 1.1e-34, (38.6% identity in 350 aa overlap). Contains prokaryotic-type class I peptide chain release factors signature (PS00745). Belongs to the prokaryotic and mitochondrial release factors family. P9WHG1 No information pathways P66026 P9WHG1 GO:0016149,GO:0006415,GO:0005737 P9WHG1 NC_000962.3 Mycobrowser_v5 CDS 3475378 3476961 . - 0 Rv3107c agpS Involved in ether lipid biosynthesis [catalytic activity: 1-acyl-glycerone 3-phosphate + a long-chain alcohol = 1-alkyl-glycerone 3-phosphate + a long-chain acid anion]. Possible alkyldihydroxyacetonephosphate synthase AgpS (alkyl-DHAP synthase) (alkylglycerone-phosphate synthase) Rv3107c, (MTCY164.17c), len: 527 aa. Possible agpS, alkyl-dihydroxyacetonephosphate synthase, similar to others and some various enzymes e.g. AAK46595|MT2311 putative alkyl-dihydroxyacetonephosphate synthase from Mycobacterium tuberculosis strain CDC1551 (529 aa), FASTA scores: opt: 1052, E(): 2.1e-58, (37.1% identity in 542 aa overlap); Q9RJ97|SCF91.28c putative flavoprotein from Streptomyces coelicolor (530 aa), FASTA scores: opt: 972, E(): 2.2e-53, (36.2% identity in 544 aa overlap); O96759|ADAS_DICDI alkyldihydroxyacetonephosphate synthase from Dictyostelium discoideum (Slime mold) (611 aa), FASTA scores: opt: 617, E(): 4.5e-31, (33.95% identity in 480 aa overlap); O97157|ADAS_TRYBB alkyldihydroxyacetonephosphate synthase from Trypanosoma brucei (613 aa), FASTA scores: opt: 567, E(): 6.2e-28, (29.15% identity in 521 aa overlap); etc. Also similar to O53525|Rv2251|MTV022.01 hypothetical 49.8 KDA protein from Mycobacterium tuberculosis strain H37Rv (475 aa), FASTA scores: opt: 1019, E(): 2.3e-56, (38.6% identity in 487 aa overlap). Belongs to the FAD-binding oxidoreductase/transferase family 4. Cofactor: FAD (by similarity). O05784 No lipid metabolism O05784 O05784 GO:0008609,GO:0016491,GO:0050660 2.5.1.26 O05784 NC_000962.3 Mycobrowser_v5 CDS 3477649 3478728 . + 0 Rv3109 moaA1 Involved in molybdenum cofactor biosynthesis; involved in the biosynthesis of molybdopterin precursor Z from guanosine. Probable molybdenum cofactor biosynthesis protein A MoaA1 Rv3109, (MTCY164.19), len: 359 aa. Probable moaA1, molybdenum cofactor biosynthesis protein, highly similar to others e.g. P39757|MOAA_BACSU|NARA|NARAB from Bacillus subtilis (341 aa), FASTA scores: opt: 810, E(): 6.2e-44, (39.75% identity in 327 aa overlap); O67929|MOAA_AQUAE|AQ_2183 from Aquifex aeolicus (320 aa), FASTA scores: opt: 794, E(): 6e-43, (40.55% identity in 323 aa overlap); Q9ZIM6|MOAA_STACA from Staphylococcus carnosus (340 aa), FASTA scores: opt: 783, E(): 3.2e-42, (38.65% identity in 326 aa overlap); etc. Also highly similar to O53143|MOAA3|MOA3_MYCTU|MT3427 molybdenum cofactor biosynthesis protein A 3 from Mycobacterium tuberculosis strain F4 (378 aa), FASTA scores: opt: 1762, E(): 4.7e-104, (74.3% identity in 350 aa overlap); and similar to O53881|MOA2_MYCTU|MOAA2|Rv0869c|MT0892|MTV043.62 molybdenum cofactor biosynthesis protein A 2 from Mycobacterium tuberculosis strain H37Rv (360 aa), FASTA scores: opt: 657, E(): 3e-34, (36.55% identity in 309 aa overlap). Belongs to the MoaA / NifB / PqqE family. Note that previously known as moaA. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WJS3 No intermediary metabolism and respiration O05786 P9WJS3 GO:0005525,GO:0006777,GO:0003824,GO:0046872,GO:0019008,GO:0051539 P9WJS3 NC_000962.3 Mycobrowser_v5 CDS 3480759 3481289 . + 0 Rv3114 Rv3114 Function unknown Conserved hypothetical protein Rv3114, (MTCY164.24), len: 176 aa. Conserved hypothetical protein, with some similarity to Q9F9W7 cytosine deaminase from Bifidobacterium longum (143 aa), FASTA scores: opt: 207, E(): 2.2e-07, (37.05% identity in 108 aa overlap); and Q9RV23|DR1207 cell cycle protein MESJ, putative/cytosine deaminase-related protein from Deinococcus radiodurans (600 aa), FASTA scores: opt: 212, E(): 3.5e-07, (33.35% identity in 177 aa overlap). Equivalent to AAK47536|MT3196 cytidine and deoxycytidylate deaminase family protein from Mycobacterium tuberculosis strain CDC1551 (187 aa) but shorter 11 aa. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O05791 No conserved hypotheticals O05791 O05791 GO:0008270,GO:0016787 O05791 NC_000962.3 Mycobrowser_v5 CDS 3482776 3483945 . + 0 Rv3116 moeB2 Possibly involved in molybdopterin metabolism (synthesis). Probable molybdenum cofactor biosynthesis protein MoeB2 (MPT-synthase sulfurylase) (molybdopterin synthase sulphurylase) Rv3116, (MTCY164.26), len: 389 aa. Probable moeB2, molybdopterin cofactor biosynthesis protein, equivalent to Q9CCG8|MOEZ|ML0817 protein probably involved in molybdopterin biosynthesis from Mycobacterium leprae (395 aa), FASTA scores: opt: 1433, E(): 8e-80, (57.8% identity in 384 aa overlap). Very similar to members of the HESA/MOEB/THIF family e.g. Q9FCL0|2SC3B6.02 putative sulfurylase from Streptomyces coelicolor (392 aa), FASTA scores: opt: 1562, E(): 1.1e-87, (58.15% identity in 380 aa overlap); Q9XC37|PDTORFF MOEB-like protein (putative sulfurylase) from Pseudomonas stutzeri (Pseudomonas perfectomarina) (391 aa), FASTA scores: opt: 1311, E(): 2.1e-72, (52.4% identity in 395 aa overlap); O54307|MPT|MOEB MPT-synthase sulfurylase from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (391 aa), FASTA scores: opt: 1238, E(): 5.7e-68, (51.4% identity in 393 aa overlap); P74344|MOEB|SLL1536 molybdopterin biosynthesis MOEB protein from Synechocystis sp. strain PCC 6803 (392 aa), FASTA scores: opt: 1212, E(): 2.2e-66, (46.5% identity in 398 aa overlap); etc. Also highly similar to O05860|MTCY07D11.20|MOEB1|Rv3206c putative molybdenum cofactor biosynthesis protein from Mycobacterium tuberculosis strain H37Rv (392 aa), FASTA scores: opt: 1445, E(): 1.5e-80, (56.25% identity in 400 aa overlap). Belongs to the HesA /MoeB/ThiF family. Note that previously known as moeB. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L7N674 No intermediary metabolism and respiration Q7D637 L7N674 GO:0003824,GO:0008152 L7N674 NC_000962.3 Mycobrowser_v5 CDS 3484809 3485111 . + 0 Rv3118 sseC1 Thought to be involved in sulphur metabolism. Conserved hypothetical protein SseC1 Rv3118, (MTCY164.28, O05794), len: 100 aa. SseC1, conserved hypothetical protein, equivalent to Q9CBC7|ML2199 hypothetical protein from Mycobacterium leprae (100 aa), FASTA scores: opt: 545, E(): 3.1e-30, (84.0% identity in 10 aa overlap). Also similar to hypothetical proteins e.g. Q50035 from Saccharopolyspora erythraea (Streptomyces erythraeus) (101 aa), FASTA scores: opt: 345, E(): 9.7e-17, (57.15% identity in 98 aa overlap); and Q9K4H3|SCD66.02 from Streptomyces coelicolor (95 aa), FASTA scores: opt: 249, E(): 2.8e-10, (48.5% identity in 99 aa overlap). Some weak similarity with Q9ZB84|PCAG protocatechuate 3,4-dioxygenase alpha-subunit from Pseudomonas marginata (196 aa), FASTA scores: opt: 109, E(): 1.4, (31.3% identity in 83 aa overlap); and other bacterial proteins. Identical second copy present as Rv0814c|AL022004|MTV043.06c|SSEC2 from Mycobacterium tuberculosis (100 aa) (100.0% identity in 100 aa overlap). Note that previously known as sseC. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P0CG96 No intermediary metabolism and respiration Q7D986 P0CG96 NC_000962.3 Mycobrowser_v5 CDS 3485132 3485575 . + 0 Rv3119 moaE1 Possibly a molybdenum biosynthesis cofactor. Conversion of molybdopterin precursor Z into molybdopterin requires transfer of two sulfur atoms to precursor Z (to generate the dithiolene group). This is catalyzed by the converting factor composed of a small and large subunit. Probable molybdenum cofactor biosynthesis protein E MoaE1 (molybdopterin converting factor large subunit) (molybdopterin [MPT] converting factor, subunit 2) Rv3119, (MTCY164.29), len: 147 aa. Probable moaE1, molybdopterin converting factor E (molybdopterin converting factor (subunit 2)), highly similar to others e.g. O31705|MOAE from Bacillus subtilis (157 aa), FASTA scores: opt: 390, E(): 8.6e-19, (43.95% identity in 132 aa overlap); Q9K8I7|MOAE|BH3019 from Bacillus halodurans (156 aa), FASTA scores: opt: 369, E(): 2e-17, (42.4% identity in 132 aa overlap); P30749|MOAE_ECOLI|CHLA5|B0785 from Escherichia coli strain K12 (149 aa), FASTA scores: opt: 312, E(): 1.1e-13, (38.45% identity in 130 aa overlap); etc. Also highly similar (but shorter 74 aa) to O53375|GPHA|Rv3323c|MTV016.23c MOAD-MOAE fusion protein from Mycobacterium tuberculosis (221 aa), FASTA scores: opt: 733, E(): 3.9e-41, (76.2% identity in 143 aa overlap); and highly similar to O53878|MOAE2|Rv0866|MTV043.59 putative molybdopterin synthase large subunit from Mycobacterium tuberculosis (141 aa), FASTA scores: opt: 321, E(): 2.6e-14, (40.9% identity in 132 aa overlap). Note that previously known as moaE. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WJR3 No intermediary metabolism and respiration 2WP4 O05795 P9WJR3 GO:0016740,GO:0006777 2.-.-.- P9WJR3 NC_000962.3 Mycobrowser_v5 CDS 3490476 3491651 . - 0 Rv3125c PPE49 Function unknown PPE family protein PPE49 Rv3125c, (MTCY164.35c), len: 391 aa. PPE49, Member of the Mycobacterium tuberculosis PPE family, similar to other e.g. P95247|Rv2352c|MTCY98.21c (391 aa), FASTA scores: opt: 1576, E(): 3.8e-72, (62.55% identity in 398 aa overlap), MTCY98.0029c, MTCY03A2.22c, MTCY10G2.10, MTCY02B10.25c, MTCI364.08, M TCY21C12.09c, MTCY48.17. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WHY5 No PE/PPE Q7D631 P9WHY5 EMB P9WHY5 NC_000962.3 Mycobrowser_v5 CDS 3496551 3497549 . + 0 Rv3131 Rv3131 Function unknown Conserved protein Rv3131, (MTCY03A2.27c), len: 332 aa. Conserved protein, similar to other hypothetical bacterial proteins e.g. O53476|Rv2032|MTV018.19 (331 aa), FASTA scores: opt: 568, E(): 2.5e-27, (36.7% identity in 321 aa overlap); O05800|Rv3127|MTCY164.37 (344 aa), FASTA scores: opt: 521, E(): 1.9e-24, (34.95% identity in 326 aa overlap); Q9RI33|SCJ12.27c from Streptomyces coelicolor (335 aa), FASTA scores: opt: 441, E(): 1.3e-19, (35.75% identity in 319 aa overlap); Q9RI44|SCJ12.14 from Streptomyces coelicolor (309 aa), FASTA scores: opt: 328, E(): 9.3e-13, (27.9% identity in 308 aa overlap); Q9CBP5|ML1751 from Mycobacterium leprae (721 aa), FASTA scores: opt: 137, E(): 0.78, (26.15% identity in 298 aa overlap); etc. Equivalent to AAK47555 from Mycobacterium tuberculosis strain CDC1551 but shorter 12 aa. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WIZ7 No conserved hypotheticals P95195 P9WIZ7 GO:0016491,GO:0055114 P9WIZ7 NC_000962.3 Mycobrowser_v5 CDS 3499943 3500749 . - 0 Rv3134c Rv3134c Function unknown. Could play a role in the adaptation to hypoxia, participating in the phosphorelay in the two component regulatory system DEVR|Rv3133c/DEVS|Rv3132c. Universal stress protein family protein Rv3134c, (MTCY03A2.240, len: 268 aa. Universal stress protein family protein. Ala-, Val- rich (see citations below), related to other hypothetical Mycobacterium tuberculosis proteins e.g. O53474|Rv2028c|MTV018.15c (279 aa), FASTA scores: opt: 562, E(): 3.2e-28, (40.65% identity in 273 aa overlap); O06188|Rv2624c|MTCY01A10.08 (272 aa), FASTA scores: opt: 458, E(): 1.1e-21, (36.55% identity in 271 aa overlap); O53472|R2026c|MTV018.13c (294 aa), FASTA scores: opt: 232, E(): 1.9e-07, (30.45% identity in 276 aa overlap); etc. Shares some similarity with other hypothetical proteins from Streptomyces coelicolor e.g. Q9RIZ8|SCJ1.16c (294 aa), FASTA scores: opt: 207, E(): 6.9e-06, (28.9% identity in 263 aa overlap); Q9K4L5|SC5F8.09 putative stress-inducible protein (312 aa), FASTA scores: opt: 204, E(): 1.1e-05, (28.4% identity in 271 aa overlap); etc. Equivalent to AAK47558|MT3220 Universal stress protein family from Mycobacterium tuberculosis strain CDC1551 (268 aa). Rv3134c seems cotranscribed with devR-devS (see Sherman et al., 2001). P9WFD3 No virulence, detoxification, adaptation P95192 P9WFD3 GO:0006950 P9WFD3 NC_000962.3 Mycobrowser_v5 CDS 3505363 3506769 . + 0 Rv3139 fadE24 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE24 Rv3139, (MTCY03A2.19c), len: 468 aa. Probable fadE24, acyl-CoA dehydrogenase (1.3.99.-), equivalent to O32890|MLCB1779.30|FADE24|ML0661 putative acyl-CoA dehydrogenase from Mycobacterium leprae (465 aa), FASTA scores: opt: 2587, E(): 4e-153, (83.6% identity in 464 aa overlap). Similar to other e.g. Q9HUH0|PA4995 from Pseudomonas aeruginosa (429 aa), FASTA scores: opt: 1139, E(): 2.8e-63, (45.3% identity in 426 aa overlap); Q9K6D0|MMGC|BH3799 from Bacillus halodurans (379 aa), FASTA scores: opt: 603, E(): 4.7e-30, (30.3% identity in 366 aa overlap); Q9K6D1|ACDA|BH3798 from Bacillus halodurans (380 aa), FASTA scores: opt: 601, E(): 6.3e-30, (32.25% identity in 363 aa overlap); etc. Contains acyl-CoA dehydrogenases signature 2 (PS00073) near C-terminus. Belongs to the acyl-CoA dehydrogenases family. P95187 No lipid metabolism P95187 P95187 GO:0003995,GO:0055114,GO:0050660 1.3.99.- INH P95187 NC_000962.3 Mycobrowser_v5 CDS 3508095 3509066 . + 0 Rv3141 fadB4 Involved in lipid degradation [catalytic activity: NADPH + quinone = NADP(+) + semiquinone]. Probable NADPH quinone oxidoreductase FadB4 (NADPH:quinone reductase) (zeta-crystallin) Rv3141, (MTCY03A2.17c), len: 323 aa. Probable fadB4, quinone oxidoreductase, showing strong similarity to variety of quinone oxidoreductases and domains in polyketide and fatty acid synthases e.g. Q9HTV6|PA5234 probable oxidoreductase from Pseudomonas aeruginosa (325 aa), FASTA scores: opt: 737, E(): 1.4e-35, (39.65% identity in 328 aa overlap); Q9RYQ7|DRA0251 putative NADPH quinone oxidoreductase from Deinococcus radiodurans (336 aa), FASTA scores: opt: 688, E(): 1e-32, (40.6% identity in 325 aa overlap); Q9RVG8|DR1061 putative NADPH quinone oxidoreductase from Deinococcus radiodurans (388 aa), FASTA scores: opt: 559, E(): 3.3e-25, (36.3% identity in 325 aa overlap); BAB49685|MLL2594 probable quinone oxidoreductase from Rhizobium loti (Mesorhizobium loti) (326 aa), FASTA scores: opt: 519, E(): 5.9e-23, (34.25% identity in 330 aa overlap); Q9LXZ4|T5P19_110 quinone reductase-like protein from Arabidopsis thaliana (348 aa), FASTA scores: opt: 517, E(): 8.1e-23, (33.55% identity in 322 aa overlap); etc. Also similar to Q9AA38|CC0770 zinc-containing alcohol dehydrogenase from Caulobacter crescentus (325 aa), FASTA scores: opt: 673, E(): 7.2e-32, (40.2% identity in 326 aa overlap); and Q9ABX4|CC0096 zinc-containing alcohol dehydrogenase from Caulobacter crescentus (332 aa), FASTA scores: opt: 623, E(): 5.7e-29, (40.7% identity in 334 aa overlap). Also resembles Mycobacterium tuberculosis proteins P96826|Rv0149|MTCI5_23, MTCY13D12.11, MTCY24G1.03, MTCY19H9.01. Belongs to the zinc-containing alcohol dehydrogenase family, quinone oxidoreductase subfamily. Thought to be differentially expressed within host cells (see Triccas et al., 1999). P95185 No lipid metabolism P95185 P95185 GO:0055114,GO:0008270,GO:0003960 1.6.5.5 P95185 NC_000962.3 Mycobrowser_v5 CDS 3512628 3513338 . + 0 Rv3147 nuoC Involved in aerobic|anaerobic respiration [catalytic activity: NADH + ubiquinone = NAD(+) + ubiquinol]. Probable NADH dehydrogenase I (chain C) NuoC (NADH-ubiquinone oxidoreductase chain C) Rv3147, (MTCY03A2.11c), len: 236 aa. Probable nuoC, NADH dehydrogenase, chain C, similar to others e.g. Q9XAQ6|NUOC from Streptomyces coelicolor (251 aa), FASTA scores: opt: 1113, E(): 2.6e-64, (67.35% identity in 236 aa overlap); Q9A6X2|CC1954 from Caulobacter crescentus (197 aa), FASTA scores: opt: 351, E(): 1.6e-15, (41.65% identity in 132 aa overlap); BAB48757|MLL1369 from Rhizobium loti (Mesorhizobium loti) (201 aa), FASTA scores: opt: 347, E(): 3e-15, (42.4% identity in 132 aa overlap); etc. Also similar to Q9UUU0|NUGM NUGM protein precursor from Yarrowia lipolytica (Candida lipolytica) (281 aa), FASTA scores: opt: 356, E(): 1.1e-15, (34.55% identity in 162 aa overlap). Also similar to MTCY251.05, FASTA score: E():4.9e-05. Equivalent to AAK47574 from Mycobacterium tuberculosis strain CDC1551 but longer 26 aa. Belongs to the complex I 30 KDA subunit family. P9WJH3 No intermediary metabolism and respiration P65571 P9WJH3 GO:0055114,GO:0019684,GO:0005886,GO:0048038,GO:0006810,GO:0008137 1.6.99.5 P9WJH3 NC_000962.3 Mycobrowser_v5 CDS 3515412 3516749 . + 0 Rv3150 nuoF Involved in aerobic|anaerobic respiration [catalytic activity: NADH + ubiquinone = NAD(+) + ubiquinol]. Probable NADH dehydrogenase I (chain F) NuoF (NADH-ubiquinone oxidoreductase chain F) Rv3150, (MTCY03A2.08c), len: 445 aa. Probable nuoF, NADH dehydrogenase, chain F, similar to others e.g. Q9XAQ9|NUOF_STRCO from Streptomyces coelicolor (449 aa), FASTA scores: opt: 2314, E(): 3.5e-139, (76.25% identity in 434 aa overlap); NUF2_RHIME from Rhizobium meliloti (421 aa), FASTA scores: opt: 1545, E(): 1.8e-90, (53.1% identity in 424 aa overlap); Q9RU92|DR1500 from Deinococcus radiodurans (444 aa), FASTA scores: opt: 1445, E(): 4.1e-84, (52.9% identity in 427 aa overlap); etc. Contains respiratory-chain NADH dehydrogenase 51 Kd subunit signature 2 (PS00645). Belongs to the complex I 51 KDA subunit family. Cofactor: FMN and one 4FE-4S cluster (probable). P9WIV7 No intermediary metabolism and respiration P65567 P9WIV7 GO:0010181,GO:0051287,GO:0008137,GO:0046872,GO:0055114,GO:0048038,GO:0051539 1.6.99.5 P9WIV7 NC_000962.3 Mycobrowser_v5 CDS 3519282 3520514 . + 0 Rv3152 nuoH Involved in aerobic|anaerobic respiration [catalytic activity: NADH + ubiquinone = NAD(+) + ubiquinol]. Probable NADH dehydrogenase I (chain H) NuoH (NADH-ubiquinone oxidoreductase chain H) Rv3152, (MTCY03A2.06c), len: 410 aa. Probable nuoH, integral membrane NADH dehydrogenase I, chain H, similar to others e.g. Q9XAR1 Q9XAR1|NUOH from Streptomyces coelicolor (467 aa), FASTA scores: opt: 1630, E(): 3.4e-90, (58.35% identity in 413 aa overlap); Q9RU94|DR1498 from Deinococcus radiodurans (397 aa), FASTA scores: opt: 1081, E(): 2e-57, (45.5% identity in 391 aa overlap); Q9ZCF7|NUOH_RICPR|RP796 from Rickettsia prowazekii (339 aa), FASTA scores: opt: 976, E(): 3.4e-51, (46.2% identity in 329 aa overlap); etc. Contains respiratory-chain NADH dehydrogenase subunit 1 signature 2 (PS00668). Some similarity to MTCY251.02 (FASTA score: E(): 1.2e-07). Belongs to the complex I subunit 1 family. P9WIX1 No intermediary metabolism and respiration P65561 P9WIX1 GO:0016021,GO:0055114,GO:0005886,GO:0048038,GO:0050136 1.6.99.5 NC_000962.3 Mycobrowser_v5 CDS 3521139 3521927 . + 0 Rv3154 nuoJ Involved in aerobic|anaerobic respiration [catalytic activity: NADH + ubiquinone = NAD(+) + ubiquinol]. Probable NADH dehydrogenase I (chain J) NuoJ (NADH-ubiquinone oxidoreductase chain J) Rv3154, (MTCY03A2.04c), len: 262 aa. Probable nuoJ, transmembrane NADH dehydrogenase I, chain J, similar to others e.g. Q9XAR3|NUOJ from Streptomyces coelicolor (285 aa), FASTA scores: opt: 991, E(): 3.2e-52, (63.7% identity in 243 aa overlap); Q9JX90|NUOJ|NMA0006 from Neisseria meningitidis (serogroup A) (223 aa), FASTA scores: opt: 329, E(): 9.6e-13, (34.85% identity in 175 aa overlap); Q9K1B2|NMB0253 from Neisseria meningitidis (serogroup B) (223 aa), FASTA scores: opt: 326, E(): 1.5e-12, (34.85% identity in 175 aa overlap); etc. But also similarity with Q00243|NU6C_PLEBO|NDH6 NADH-plastoquinone oxidoreductase chain 6 homolog (catalytic activity: NADH + plastoquinone = NAD(+) + plastoquinol) from Plectonema boryanum (199 aa), FASTA scores: opt: 287, E(): 2.8e-10, (34.35% identity in 195 aa overlap). Similar to polypeptide 6 of the NADH-ubiquinol oxidoreductase of chloroplasts or mitochondria. P95172 No intermediary metabolism and respiration P95172 P95172 GO:0055114,GO:0008137 1.6.5.3 P95172 NC_000962.3 Mycobrowser_v5 CDS 3521924 3522223 . + 0 Rv3155 nuoK Involved in aerobic|anaerobic respiration [catalytic activity: NADH + ubiquinone = NAD(+) + ubiquinol]. Probable NADH dehydrogenase I (chain K) NuoK (NADH-ubiquinone oxidoreductase chain K) Rv3155, (MTCY03A2.03c), len: 99 aa. Probable nuoK, integral membrane NADH dehydrogenase I, chain K, similar to others e.g. Q9XAR4|NUOK from Streptomyces coelicolor (99 aa), FASTA scores: opt: 509, E(): 2.7e-31, (78.55% identity in 98 aa overlap); Q56226|NQOB_THETH|NQO11 from Thermus aquaticus (subsp. thermophilus) (95 aa), blast scores: initn: 298, init1: 180, bits: 85.7, FASTA scores: opt: 313, E(): 9.4e-17, (53.7% identity in 95 aa overlap); Q9RU97|DR1495 from Deinococcus radiodurans (103 aa), FASTA scores: opt: 309, E(): 2e-16, (52.0% identity in 100 aa overlap); etc. But also similarity with NADH-plastoquinone oxidoreductases chain 4L e.g. Q9MUL4|NULC_MESVI|NDHE from Mesostigma viride (catalytic activity: NADH + plastoquinone = NAD(+) + plastoquinol) (101 aa), FASTA scores: opt: 280, E(): 2.8e-14, (40.6% identity in 101 aa overlap); and P06261|NULC_TOBAC|NDHE|NDH4L from Nicotiana tabacum (Common tobacco) (101 aa), FASTA scores: opt: 259, E(): 1e-12, (43.0% identity in 93 aa overlap). Similar to polypeptide 4L of the NADH-ubiquinol oxidoreductase of chloroplasts or mitochondria. P9WIX3 No intermediary metabolism and respiration P65565 P9WIX3 GO:0050136,GO:0016021,GO:0019684,GO:0005886,GO:0048038,GO:0006810,GO:0042773 1.6.99.5 P9WIX3 NC_000962.3 Mycobrowser_v5 CDS 3522234 3524135 . + 0 Rv3156 nuoL Involved in aerobic|anaerobic respiration [catalytic activity: NADH + ubiquinone = NAD(+) + ubiquinol]. Probable NADH dehydrogenase I (chain L) NuoL (NADH-ubiquinone oxidoreductase chain L) Rv3156, (MTCY03A2.02c), len: 633 aa. Probable nuoL, integral membrane NADH dehydrogenase I, chain L, similar to others e.g. Q9XAR5|NUOL_STRCO from Streptomyces coelicolor (654 aa), FASTA scores: opt: 2074, E(): 1.1e-111, (61.1% identity in 648 aa overlap); Q56227|NQOC_THETH|NQO12 from Thermus aquaticus (subsp. thermophilus) (606 aa), FASTA scores: opt: 1420, E(): 3.8e-74, (43.35% identity in 630 aa overlap); Q9ZJV6|NUOL|JHP1192 from Helicobacter pylori J99 (Campylobacter pylori J99) (612 aa), FASTA scores: opt: 1279, E(): 4.7e-66, (41.65% identity in 516 aa overlap); etc. Also similar to MTCY251.04 (FASTA score: E(): 1.3e-11) and MTCY03A2.01c (FASTA score: E(): 2.3e-10). Similar to polypeptide 5 of the NADH-ubiquinol oxidoreductase of chloroplasts or mitochondrial. P9WIW1 No intermediary metabolism and respiration O86350 P9WIW1 GO:0008137,GO:0016021,GO:0005886,GO:0048038,GO:0042773 1.6.99.5 P9WIW1 NC_000962.3 Mycobrowser_v5 CDS 3525790 3527385 . + 0 Rv3158 nuoN Involved in aerobic|anaerobic respiration [catalytic activity: NADH + ubiquinone = NAD(+) + ubiquinol]. Probable NADH dehydrogenase I (chain N) NuoN (NADH-ubiquinone oxidoreductase chain N) Rv3158, (MTV014.02c), len: 531 aa. Probable nuoN, integral membrane NADH dehydrogenase I, chain N, similar to others e.g. Q9XAR7|SC10A7.08c from Streptomyces coelicolor (552 aa), FASTA scores: opt: 1493, E(): 1.1e-81, (56.7% identity in 543 aa overlap); Q9PGI2|XF0318 from Xylella fastidiosa (485 aa), FASTA scores: opt: 942, E(): 7.4e-49, (39.6% identity in 379 aa overlap); CAB51628|NUON2 from Rhizobium meliloti (Sinorhizobium meliloti) (479 aa), FASTA scores: opt: 934, E(): 2.2e-48, (35.5% identity in 479 aa overlap); etc. But also similarity with NADH-plastoquinone oxidoreductases chain 4L (catalytic activity: NADH + plastoquinone = NAD(+) + plastoquinol) e.g. P29801|NU2C_SYNP7|NDHB from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (521 aa), FASTA scores: opt: 921, E(): 1.4e-47, (40.25% identity in 395 aa overlap). Belongs to the complex I subunit 2 family. P9WIW9 No intermediary metabolism and respiration P0A5M0 P9WIW9 GO:0008137,GO:0016021,GO:0019684,GO:0005886,GO:0048038,GO:0006810,GO:0042773 1.6.99.5 NC_000962.3 Mycobrowser_v5 CDS 3535431 3536057 . - 0 Rv3167c Rv3167c Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably TetR-family) Rv3167c, (MTV014.11c), len: 208 aa. Probable transcriptional regulator, TetR family, similar to several transcriptional regulators e.g. Q9L2A4|SC8F4.22c (TetR/AcrR family) from Streptomyces coelicolor (234 aa), FASTA scores: opt: 317, E(): 7.5e-13, (33.35% identity in 210 aa overlap); Q9RK47|SCF12.11 (TetR/AcrR family) from Streptomyces coelicolor (206 aa), FASTA scores: opt: 293, E(): 2.1e-11, (32.65% identity in 199 aa overlap); Q54288 regulator of antibiotic transport complexes (TetR/AcrR family) (204 aa), FASTA scores: opt: 260, E(): 2.4e-09, (30.75% identity in 205 aa overlap); etc. Equivalent to AAK47595 from Mycobacterium tuberculosis strain CDC1551 but shorter 21 aa. Contains probable helix-turn-helix motif from aa 42 to 63 (Score 1727, +5.07 SD). May belong to the TetR/AcrR family of transcriptional regulators. O53317 No regulatory proteins O53317 O53317 GO:0006350,GO:0003700,GO:0006355 O53317 NC_000962.3 Mycobrowser_v5 CDS 3538505 3539851 . + 0 Rv3170 aofH Possibly catalyzes the oxidative deamination: oxidize on primary amines, and perhaps on secondary and tertiary amines [catalytic activity: RCH(2)NH(2) + H(2)O + O(2) = RCHO + NH(3) + H(2)O(2)]. Must have important function in metabolism. Supposedly involved in stationary-phase survival. Probable flavin-containing monoamine oxidase AofH (amine oxidase) (MAO) Rv3170, (MT3259, MTV014.14), len: 448 aa. Probable aofH, flavin-containing (mono)amine oxidase, equivalent to a predicted homologous protein from Mycobacterium smegmatis (see citation below), and similar to many eukaryotic monoamine oxidases e.g. P49253|AOF_ONCMY from Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri) (522 aa), FASTA scores: opt: 869, E(): 5.3e-44, (37.7% identity in 448 aa overlap); P21396|AOFA_RAT|MAOA from Rattus norvegicus (Rat) (526 aa), FASTA scores: opt: 839, E(): 3.2e-42, (37.45% identity in 446 aa overlap); Q99NA8|MAO-a from Cavia porcellus (Guinea pig) (506 aa), FASTA scores: opt: 836, E(): 4.6e-42, (37.0% identity in 446 aa overlap); P21398|AOFA_BOVIN from Bos taurus (Bovine) (527 aa), FASTA scores: opt: 806, E(): 2.8e-40, (37.0% identity in 446 aa overlap); P21397|AOFA_HUMAN (527 aa), FASTA scores: opt: 801, E(): 5.6e-40, (37.2% identity in 446 aa overlap); etc. Alternative start possible at position 3538487. Belongs to the flavin monoamine oxidase family. Cofactor: FAD (potential). P9WQ15 No intermediary metabolism and respiration P63533 P9WQ15 GO:0016491,GO:0055114 1.4.3.- P9WQ15 NC_000962.3 Mycobrowser_v5 CDS 3539846 3540745 . - 0 Rv3171c hpx Supposedly involved in detoxification reactions. Possible non-heme haloperoxidase Hpx Rv3171c, (MTV014.15c), len: 299 aa. Possible hpx, non-heme haloperoxidase, similar to other hydrolases (principaly epoxide hydrolases) and non-heme chloroperoxidases e.g. Q9RKB6|SCE87.22c putative hydrolase from Streptomyces coelicolor (314 aa), FASTA scores: opt: 431, E(): 6e-20, (38.05% identity in 297 aa overlap); Q9HZ14|PA3226 probable hydrolase (similar to alpha/beta hydrolase fold) from Pseudomonas aeruginosa (275 aa), FASTA scores: opt: 236, E(): 1e-07, (29.6% identity in 277 aa overlap); Q9DBL9|1300003 D03RIK protein similar to alpha/beta hydrolase fold from Mus musculus (Mouse) (351 aa), FASTA scores: opt: 223, E(): 8.3e-07, (24.35% identity in 304 aa overlap); AAK46260|MT1988 epoxide hydrolase from Mycobacterium tuberculosis strain CDC1551 (356 aa), FASTA scores: opt: 223, E(): 8.4e-07, (40.7% identity in 113 aa overlap); P49323|PRXC_STRLI|CPO|CPOL non-heme chloroperoxidase (chloride peroxidase) from Streptomyces lividans (275 aa), FASTA scores: opt: 220, E(): 1e-06, (29.5% identity in 305 aa overlap); etc. Equivalent to AAK47599 Hydrolase, alpha/beta hydrolase family from Mycobacterium tuberculosis strain CDC1551 but shorter 24 aa. Start chosen by similarity, alternative with good RBS possible. O53321 No virulence, detoxification, adaptation O53321 O53321 GO:0004601,GO:0055114 1.11.1.- O53321 NC_000962.3 Mycobrowser_v5 CDS 3542860 3544347 . + 0 Rv3175 Rv3175 Function unknown; involved in cellular metabolism. Possible amidase (aminohydrolase) Rv3175, (MTV014.19), len: 495 aa. Possible amidase , similar to others e.g. Q9F6D0|ZHUL enantiomer selective amidase from Streptomyces sp. R1128 (507 aa), FASTA scores: opt: 1328 ,E(): 7.5e-69, (44.5% identity in 492 aa overlap); BAB51815|MLR5350 probable amidase from Rhizobium loti (Mesorhizobium loti) (457 aa), FASTA scores: opt: 7487, E(): 1.3e-35, (35.9% identity in 482 aa overlap); O28325|YJ54_ARCFU|AF1954 putative amidase from Archaeoglobus fulgidus (453 aa), FASTA scores: opt: 532, E(): 3.2e-23, (32.05% identity in 471 aa overlap); etc. But also similar to glutamyl-tRNA amidotransferases who belong to amidase family e.g. Q9RTA9|DR1856 glutamyl-tRNA(GLN) amidotransferase, subunit A from Deinococcus radiodurans (482 aa), FASTA scores: opt: 560, E(): 8.2e-25, (30.6% identity in 513 aa overlap); Q9LCX3|GATA GLU/asp-tRNA amidotransferase subunit A from Thermus aquaticus (subsp. thermophilus) (471 aa), FASTA scores: opt: 558, E(): 1.1e-24, (30.85% identity in 486 aa overlap); Q49091|GATA_MORCA glutamyl-tRNA(GLN) amidotransferase subunit A from Moraxella catarrhalis (492 aa), FASTA scores: opt: 526, E(): 7.5e-23, (30.45% identity in 473 aa overlap); etc. Seems to belong to the amidase family. Contains PS00017 ATP/GTP-binding site motif A (P-loop). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53325 No intermediary metabolism and respiration O53325 O53325 GO:0016787,GO:0016884 3.5.1.- O53325 NC_000962.3 Mycobrowser_v5 CDS 3544344 3545300 . - 0 Rv3176c mesT Biotransformation enzyme that acts on a variety of epoxides and arene oxides. Catalyzes the hydrolysis of arene and epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water [catalytic activity: an epoxide + H(2)O = a glycol]. Probable epoxide hydrolase MesT (epoxide hydratase) (arene-oxide hydratase) Rv3176c, (MTV014.20c), len: 318 aa. Probable mesT, epoxide hydrolase, similar to others e.g. O15007|PEG1|MEST|Q92571|O14973 MEST protein (mesoderm specific transcript (mouse) homolog) (similar to alpha/beta hydrolase fold) from Homo sapiens (Human) (335 aa), FASTA scores: opt: 348, E(): 6e-15, (32.15% identity in 280 aa overlap); AAH06639|Q07646 MEST protein from Mus musculus (Mouse) (335 aa), FASTA scores: opt: 342, E(): 1.4e-14, (31.45% identity in 280 aa overlap); Q9I8E7|MEST epoxide hydrolase from Fugu rubripes (Japanese pufferfish) (Takifugu rubripes) (326 aa), FASTA scores: opt: 322, E(): 2.7e-13, (29.55% identity in 301 aa overlap); Q9PUC9|PEG1|MEST epoxide hydrolase from Brachydanio rerio (Zebrafish) (Zebra danio) (344 aa), FASTA scores: opt: 322, E(): 2.8e-13, (32.35% identity in 207 aa overlap); Q9HYH6|PA3429 probable epoxide hydrolase from Pseudomonas aeruginosa (298 aa), FASTA scores: opt: 258, E(): 3e-09, (29.85% identity in 288 aa overlap); O31243|ECHA epoxide hydrolase from Agrobacterium radiobacter (294 aa), FASTA scores: opt: 202, E(): 1.1e-05, (27.0% identity in 278 aa overlap); etc. Also similar to Q50599|Rv1834|MT1882|MTCY1A11.09c hypothetical 31.7 KDA protein from Mycobacterium tuberculosis (288 aa), FASTA scores: opt: 294, E(): 1.5e-11, (29.95% identity in 287 aa overlap). Equivalent to AAK47604 from Mycobacterium tuberculosis strain CDC1551 (339 aa) but shorter 21 aa. Similar to alpha/beta hydrolase fold. May belong to peptidase family S33. Note that previously known as lipS. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q6MX03 No virulence, detoxification, adaptation Q6MX03 GO:0016787 3.3.2.3 Q6MX03 NC_000962.3 Mycobrowser_v5 CDS 3557311 3558345 . - 0 Rv3191c Rv3191c Involved in the transposition of an insertion sequence. Probable transposase Rv3191c, (MTV014.35c), len: 344 aa. Probable transposase, similar to many especially Q9K2N8 putative transposase from Pseudomonas aeruginosa (338 aa), FASTA scores: opt: 837, E(): 1.3e-43, (42.55% identity in 336 aa overlap); Q9RBF4 insertion sequence IS1088 from Alcaligenes eutrophus (Ralstonia eutropha) (342 aa), FASTA scores: opt: 823, E(): 9.2e-43, (43.05% identity in 337 aa overlap); and Q51379 putative transposase from Pseudomonas alcaligenes (338 aa), FASTA scores: opt: 818, E(): 1.8e-42, (42.35% identity in 333 aa overlap). Contains probable helix-turn-helix motif from aa 25 to 46 (Score 1968, +5.89 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53337 No insertion seqs and phages O53337 O53337 GO:0015074,GO:0003677 O53337 NC_000962.3 Mycobrowser_v5 CDS 3560194 3563172 . - 0 Rv3193c Rv3193c Unknown Probable conserved transmembrane protein Rv3193c, (MTV014.37c), len: 992 aa. Probable conserved transmembrane protein, with hydrophobic N-terminal domain (~1-340 aa), highly similar to Q9CCM6|ML0644 putative integral membrane protein from Mycobacterium leprae (983 aa), FASTA scores: opt: 5421, E(): 0, (86.15% identity in 989 aa overlap); and O53609|Rv0064|MTV030.07 putative membrane protein from Mycobacterium tuberculosis strain H37Rv (979 aa), FASTA scores: opt: 3204, E(): 2.1e-142, (50.25% identity in 985 aa overlap). C-terminal part (709-990 aa) highly similar to O32904|MLCB1779.46 hypothetical 29.1 KDA protein from Mycobacterium leprae (277 aa), FASTA scores: opt: 1521, E(): 3.4e-64, (82.6% identity in 282 aa overlap). Also some similarity to hypothetical proteins generally transmembrane e.g. Q9FCI4|2SC3B6.28 from Streptomyces coelicolor (815 aa), FASTA scores: opt: 951, E(): 3.4e-37, (39.2% identity in 826 aa overlap); P72637|SLL1060 from Synechocystis sp. strain PCC 6803 (1032 aa), FASTA scores: opt: 938, E(): 1.6e-36, (29.95% identity in 855 aa overlap); O28851|AF1421 from Archaeoglobus fulgidus (880 aa), FASTA scores: opt: 526, E(): 2.6e-17, (28.05% identity in 970 aa overlap); etc. P9WFL3 No cell wall and cell processes O53339 P9WFL3 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 3567024 3568367 . + 0 Rv3197 Rv3197 Thought to be involved in active transport across the membrane. Responsible for energy coupling to the transport system. Probable conserved ATP-binding protein ABC transporter Rv3197, (MTV014.41), len: 447 aa. Probable conserved ATP-binding protein ABC transporter, highly similar to Mycobacterium leprae proteins: Q9CCM8|ML0640 hypothetical protein (473 aa), FASTA scores: opt: 2512, E(): 2.1e-140, (83.0% identity in 447 aa overlap). Interestingly, the N-terminal half (1-219 aa) corresponds to Q49747|ABC1|B1937_C3_233 ABC1 protein from Mycobacterium leprae (267 aa), FASTA scores: opt: 1276, E(): 6.3e-68, (88.6% identity in 219 aa overlap); and the C-terminal half (239-447 aa) corresponds to Q49745|B1937_C2_179 hypothetical 23.1 KDA protein (206 aa), FASTA scores: opt: 1138, E(): 6.5e-60, (77.05% identity in 209 aa overlap); two adjacent orfs from Mycobacterium leprae. Also highly similar to other proteins (generally ABC transporters) e.g. Q9FCJ6|2SC3B6.16c hypothetical 51.3 KDA protein from Streptomyces coelicolor (469 aa), FASTA scores: opt: 1340, E(): 1.8e-71, (45.9% identity in 449 aa overlap); O65576|ABC1AT ABC1 protein (alias Q9SBB2|T15B16.14|AT4G01660 putative ABC transporter) from Arabidopsis thaliana (Mouse-ear cress) (623 aa), FASTA scores: opt: 543, E(): 1.7e-24, (28.4% identity in 405 aa overlap); O27682|MTH1645 ABC transporter from Methanobacterium thermoautotrophicum (623 aa), FASTA scores: opt: 497, E(): 7.8e-22, (33.0% identity in 309 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters). O53343 No cell wall and cell processes O53343 O53343 GO:0005524 O53343 NC_000962.3 Mycobrowser_v5 CDS 3568401 3568679 . - 0 Rv3197A whiB7 Involved in transcriptional mechanism. Probable transcriptional regulatory protein WhiB-like WhiB7 Rv3197A, len: 92 aa. Probable whiB7 (alternate gene name: whmC), WhiB-like regulatory protein (see citation below), similar to WhiB paralogue of Streptomyces coelicolor, wblE gene product (85 aa). Equivalent to Q49765|WHIB7|ML0639|B1937_F2_68 putative transcriptional regulator WHIB7 from Mycobacterium leprae (89 aa), FASTA scores: opt: 441, E(): 6.3e-24, (69.3% identity in 88 aa overlap). Similar to Q9FCJ8|2SC3B6.14 putative DNA-binding protein from Streptomyces coelicolor (122 aa), FASTA scores: opt: 348, E(): 2.2e-17, (57.7% identity in 78 aa overlap); Q9AD55|SCP1.95 putative regulatory protein from Streptomyces coelicolor (102 aa), FASTA scores: opt: 166, E(): 7.1e-05, (39.4% identity in 76 aa overlap); etc. Q6MX01 No regulatory proteins Q6MX01 Q6MX01 GO:0003677 NC_000962.3 Mycobrowser_v5 CDS 3605751 3607034 . - 0 Rv3229c desA3 Thought to be involved in lipid metabolism [catalytic activity: linoleoyl-CoA + ah(2) + O(2) = gamma-linolenoyl-CoA + a + 2 H(2)O] Possible linoleoyl-CoA desaturase (delta(6)-desaturase) Rv3229c, (MTCY20B11.04c), len: 427 aa. DesA3, linoleoyl-CoA desaturase, showing similarity with desaturases and other proteins e.g. Q08871|DES6|SLL0262 linoleoyl-CoA desaturase from Synechocystis sp. strain PCC 6803 (359 aa), FASTA scores: opt: 319, E(): 4e-13, (25.1% identity in 295 aa overlap); Q54795|DESD delta 6 desaturase from Spirulina platensis (368 aa), FASTA scores: opt: 268, E(): 7.7e-10, (25.0% identity in 300 aa overlap); Q9ZTU8|S276 protein with similarity to cytochrome B5 domain from Triticum aestivum (Wheat) (469 aa), FASTA scores: opt: 240, E(): 5.9e-08, (27.05% identity in 266 aa overlap); etc. P9WNZ3 No lipid metabolism Q7D5W1 P9WNZ3 GO:0055114,GO:0016717,GO:0005886,GO:0006633 1.14.19.n4 NC_000962.3 Mycobrowser_v5 CDS 3571602 3572543 . - 0 Rv3199c nudC Involved in nicotinate and nicotinamide metabolism. Generates AMP and NMN from NAD(+) and H(2)O. Acting on acid anhydrides, in phosphorus-containing anhydrides. Also acts on NADP+, 3-acetylpyridine and the thionicotinamide analogues of NAD+ and NADP+ [catalytic activity: NADH + H(2)O = AMP + NMNH]. Probable NADH pyrophosphatase NudC (NAD+ diphosphatase) (NAD+ pyrophosphatase) (NADP pyrophosphatase) Rv3199c, (MTV014.43)c, len: 313 aa. Probable nudC, NADH pyrophosphatase, similar in particular to Q9CXN4|4933433B15RIK from Mus musculus (Mouse) (356 aa), FASTA scores: opt: 493, E(): 7.4e-24, (39.65% identity in 232 aa overlap); Q9ABG1|CC0266 mutt/NUDIX family protein from Caulobacter crescentus (313 aa), FASTA scores: opt: 479, E(): 5.1e-23, (38.3% identity in 222 aa overlap); O86062|NUDC_PSEAE|NUDC|PA1823 NADH pyrophosphatase from Pseudomonas aeruginosa (278 aa), FASTA scores: opt: 371,2 E(): 3e-16, (43.15% identity in 153 aa overlap); Q9RV62|NUDC_DEIRA|NUDC|DR1168 NADH pyrophosphatase from Deinococcus radiodurans (280 aa), FASTA scores: opt: 363, E(): 9.6e-16, (34.45% identity in 270 aa overlap); etc. Caution: equivalent to AAK47636 from Mycobacterium tuberculosis strain CDC1551 (386 aa) but shorter 72 aa. Contains PS00893 mutT domain signature. Belongs to the NUDIX hydrolase family, NUDC subfamily. Cofactor: requires divalent ions: manganese or magnesium. P9WIX5 No intermediary metabolism and respiration O53345 P9WIX5 GO:0000287,GO:0030145,GO:0000210 3.6.1.22 P9WIX5 NC_000962.3 Mycobrowser_v5 CDS 3572602 3573669 . - 0 Rv3200c Rv3200c Thought to be involved in cation transport across the membrane. Possible transmembrane cation transporter Rv3200c, (MTV014.44c), len: 355 aa. Possible transmembrane cation transporter, similar to many transmembrane proteins and putative potassium channels e.g. Q9XA52|SCGD3.27C putative membrane protein from Streptomyces coelicolor (365 aa), FASTA scores: opt: 1022, E(): 2.6e-53, (49.85% identity in 325 aa overlap); Q9RRZ3|DR2336 putative potassium channel from Deinococcus radiodurans (320 aa), FASTA scores: opt: 436, E(): 1e-18, (30.9% identity in 304 aa overlap); O28600|AF1673 putative potassium channel from Archaeoglobus fulgidus (314 aa), FASTA scores: opt: 363, E(): 2.1e-14, (27.2% identity in 309 aa overlap); Q57604|Y13B_METJAMJ0138.1|MJ0138.1 putative potassium channel from Methanococcus jannaschii (333 aa), FASTA scores: opt: 356, E(): 5.7e-14, (26.0% identity in 281 aa overlap); P73132|SLL0993 potassium channel from Synechocystis sp. strain PCC 6803 (365 aa), FASTA scores: opt: 330, E(): 2.1e-12, (27.8% identity in 324 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). O53346 No cell wall and cell processes O53346 O53346 GO:0003824,GO:0016021,GO:0008152,GO:0006813 O53346 NC_000962.3 Mycobrowser_v5 CDS 3582532 3583710 . - 0 Rv3206c moeB1 Possibly involved in molybdopterin metabolism (synthesis) Probable molybdenum cofactor biosynthesis protein MoeB1 (MPT-synthase sulfurylase) (molybdopterin synthase sulphurylase) Rv3206c, (MTCY07D11.20), len: 392 aa. Probable moeB1, molybdopterin cofactor biosynthesis protein, equivalent to Q9CCG8|MOEZ|ML0817 protein probably involved in molybdopterin biosynthesis from Mycobacterium leprae (395 aa), FASTA scores: opt: 2285, E(): 3.3e-130, (86.45% identity in 391 aa overlap.) Very similar to members of the HESA/MOEB/THIF family e.g. Q9FCL0|2SC3B6.02 putative sulfurylase from Streptomyces coelicolor (392 aa), FASTA scores: opt: 1776, E(): 1.4e-99, (65.3% identity in 395 aa overlap); Q9XC37|PDTORFF MOEB-like protein (putative sulfurylase) from Pseudomonas stutzeri (Pseudomonas perfectomarina) (391 aa), FASTA scores: opt: 1526, E(): 1.5e-84, (59.1% identity in 391 aa overlap); O54307|MPT|MOEB MPT-synthase sulfurylase from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (391 aa), FASTA scores: opt: 1309, E(): 1.8e-71, (52.95% identity in 387 aa overlap); P74344|MOEB|SLL1536 molybdopterin biosynthesis MOEB protein from Synechocystis sp. strain PCC 6803 (392 aa), FASTA scores: opt: 1308, E(): 2e-71, (50.65% identity in 397 aa overlap); etc. Also highly similar to O05792|MOEB2|Rv3116|MTCY164.26 putative molybdenum cofactor biosynthesis protein from Mycobacterium tuberculosis (389 aa), FASTA scores: opt: 1440, E(): 2.3e-79, (57.25% identity in 386 aa overlap). Has hydrophobic segment from ~45-71. Belongs to the HesA/MoeB/ThiF FAMILY. Note that previously known as moeZ. Thought to be differentially expressed within host cells (see citation below). P9WMN7 No intermediary metabolism and respiration Q7D5X9 P9WMN7 GO:0003824,GO:0008152 P9WMN7 NC_000962.3 Mycobrowser_v5 CDS 3583801 3584658 . - 0 Rv3207c Rv3207c Function unknown Conserved protein Rv3207c, (MTCY07D11.19), len: 285 aa. Conserved protein, highly similar but shorter (57 aa) to Q9CCG9|ML0816 hypothetical protein from Mycobacterium leprae (341 aa), FASTA scores: opt: 1676, E(): 9.7e-96, (81.0% identity in 284 aa overlap). Also similar to C-terminus of Q9FBI6|SCP8.36 hypothetical protein from Streptomyces coelicolor (559 aa), FASTA scores: opt: 426, E(): 8.4e-19, (37.35% identity in 281 aa overlap); and similar to other hypothetical proteins (generally membrane proteins) e.g. Q9K456|SC2H12.28C putative membrane protein from Streptomyces coelicolor (314 aa), FASTA scores: opt: 341, E(): 8.8e-14, (29.75% identity in 296 aa overlap). Contains neutral zinc metallopeptidases, zinc-binding region signature (PS00142). O05859 No conserved hypotheticals O05859 O05859 GO:0006508,GO:0008270,GO:0008237 O05859 NC_000962.3 Mycobrowser_v5 CDS 3585004 3585690 . + 0 Rv3208 Rv3208 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably TetR-family) Rv3208, (MTCY07D11.18c), len: 228 aa. Probable transcriptional regulator, TetR family, equivalent to Q9CCH0|ML0815 putative TetR-family transcriptional regulator from Mycobacterium leprae (228 aa), FASTA scores: opt: 1248, E(): 1.4e-74, (82.4% identity in 227 aa overlap). Also highly similar to Q9FBI8|SCP8.33c putative TetR-family transcriptional regulator from Streptomyces coelicolor (213 aa), FASTA scores: opt: 629, E(): 4e-34, (45.8% identity in 203 aa overlap); Q9KIL9|F58R F58R (fragment) from Streptomyces coelicolor A3(2) (149 aa), FASTA scores: opt: 497, E(): 1.3e-25, (50.35% identity in 147 aa overlap); Q9K3T5|SCE66.08 putative TetR-family transcriptional regulator from Streptomyces coelicolor (225 aa), FASTA scores: opt: 344, E(): 1.8e-15, (31.15% identity in 212 aa overlap); Q9RYK4|DRA0308 transcriptional regulator, TetR family from Deinococcus radiodurans (239 aa), FASTA scores: opt: 290, E(): 6.5e-12, (30.5% identity in 223 aa overlap); etc. And also similar to Mycobacterium tuberculosis proteins P96381|Rv1019|MTCY10G2.30c hypothetical 21.7 KDA protein (197 aa), FASTA scores: opt: 356, E(): 2.7e-16, (34.4% identity in 189 aa overlap); MTV034_4; MTY07A7A_3; MTV032_1; MTCY07A7_12; etc. Contains probable helix-turn-helix motif at aa 60-81 (Score 1517, +4.35 SD). Similar to the TetR/AcrR family of transcriptional regulators. O05858 No regulatory proteins O05858 O05858 GO:0006350,GO:0003700,GO:0006355 O05858 NC_000962.3 Mycobrowser_v5 CDS 3736984 3738438 . - 0 Rv3344c PE_PGRS49 Function unknown PE-PGRS family protein PE_PGRS49 Rv3344c, (MTV016.44c), len: 484 aa. PE_PGRS49, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-, ala-rich proteins (see Brennan and Delogu, 2002). Appears to be a gene fragment, should be in-frame with following ORF, MTV016.45c, frameshift required around 49595 but could not be found on checking BAC and cosmid clones. Similar to many from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. O53557|Rv3512|MTV023.19 (1079 aa), FASTA scores: opt: 1595, E(): 1.8e-54, (52.0% identity in 544 aa overlap). L0TFC2 No PE/PPE L0TFC2 NC_000962.3 Mycobrowser_v5 CDS 3587798 3589381 . + 0 Rv3211 rhlE Has a helix-destabilizing activity Probable ATP-dependent RNA helicase RhlE Rv3211, (MTCY07D11.15c), len: 527 aa. Probable rhlE, ATP-dependent RNA helicase, equivalent (but shorter 22 aa) to Q9CCH3|RHLE|ML0811 putative ATP-dependent RNA helicase from Mycobacterium leprae (544 aa), FASTA scores: opt: 2497, E(): 8.7e-131, (74.75% identity in 531 aa overlap). Also highly similar to other RNA helicases e.g. Q9FBJ2|SCP8.29c from Streptomyces coelicolor (879 aa), FASTA scores: opt: 1458, E(): 3.6e-73, (52.5% identity in 522 aa overlap); Q9DF36 from Xenopus laevis (African clawed frog) (800 aa), FASTA scores: opt: 792, E(): 2.3e-36, (37.15% identity in 385 aa overlap); Q99Z38|dead|SPY1415 from Streptococcus pyogenes (759 aa), FASTA scores: opt: 779, E(): 1.1e-35, (37.1% identity in 380 aa overlap); P33906|dead|CSDA from Klebsiella pneumoniae (642 aa), FASTA scores: opt: 768, E(): 4e-35, (43.4% identity in 387 aa overlap); etc. Contains ATP/GTP-binding site motif A (PS00017) and dead-box subfamily ATP-dependent helicases signature (PS00039). Similar to dead/DEAH box helicase family and similar to helicase C-terminal domain. O05855 No information pathways O05855 O05855 GO:0008026,GO:0003676,GO:0005524 O05855 NC_000962.3 Mycobrowser_v5 CDS 3589394 3590617 . + 0 Rv3212 Rv3212 Function unknown Conserved alanine valine rich protein Rv3212, (MTCY07D11.14c), len: 407 aa. Conserved ala-, val-rich protein, equivalent to Q9CCH4|ML0810 putative membrane protein from Mycobacterium leprae (407 aa), FASTA scores: opt: 2158, E(): 5.3e-119, (79.85% identity in 407 aa overlap). Weak similarity to several eukaryotic transcription factors e.g. P08393|ICP0_HSV11|ICP0|IE110 trans-acting transcriptional protein from Herpes simplex virus (type 1 / strain 17) (775 aa), FASTA scores: opt: 115, E(): 2, (26.9% identity in 334 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). O05854 No conserved hypotheticals O05854 NC_000962.3 Mycobrowser_v5 CDS 3590692 3591492 . - 0 Rv3213c Rv3213c Function unknown, but possibly involved in regulation of partitioning. Possible SOJ/para-related protein Rv3213c, (MTCY07D11.13), len: 266 aa. Possible soj/parA-related protein, very similar in particular to Soj/ParA proteins (and relatives) from Bacillus subtilis that inhibit the initiation of sporulation by preventing phosphorylation of Spo0A (see Quisel & Grossman 2000) e.g. Q9S228|SCI51.12c from Streptomyces coelicolor (340 aa), FASTA scores: opt: 746, E(): 1.6e-40, (48.2% identity in 249 aa overlap); Q9HT11|SOJ|PA5563 from Pseudomonas aeruginosa (262 aa), FASTA scores: opt: 649, E(): 2.1e-34, (42.2% identity in 256 aa overlap); Q9PB62|XF2282 from Xylella fastidiosa (264 aa), FASTA scores: opt: 624, E(): 8.3e-33, (42.25% identity in 251 aa overlap); Q9K5N0|SOJ_BACHD|SOJ|BH4058 from Bacillus halodurans (253 aa), FASTA scores: opt: 621, E(): 1.2e-32, (41.55% identity in 248 aa overlap); P37522|SOJ_BACSU (253 aa), FASTA scores: opt: 620, E(): 1.4e-32, (41.65% identity in 245; etc. Also similar to various mycobacterial proteins: U00021_10 from Mycobacterium leprae, MTCI125_29 from Mycobacterium tuberculosis, MLCB1351_6 from Mycobacterium leprae, MTV028_9c|Rv3918c|para probable chromosome partitioning protein from Mycobacterium tuberculosis, MSGDNAB_18 from Mycobacterium leprae. Seems to belong to the para family. O05853 No cell wall and cell processes O05853 O05853 O05853 NC_000962.3 Mycobrowser_v5 CDS 3591646 3592257 . + 0 Rv3214 gpm2 Involved in glycolysis [catalytic activity: 1,3-diphosphoglycerate + 3-phosphoglycerate = 2,3-diphosphoglycerate + 3-phosphoglycerate]. Possible phosphoglycerate mutase Gpm2 (phosphoglyceromutase) (PGAM) (BPG-dependent PGAM) Rv3214, (MTCY07D11.12c), len: 203 aa. Possible gpm2, phosphoglycerate mutase, similar to many mutases especially phosphoglycerate mutases e.g. Q9F3H5|2SCC13.14c putative mutase from Streptomyces coelicolor (198 aa), FASTA scores: opt: 487, E(): 4.4e-25, (42.25% identity in 194 aa overlap); BAB49378|MLL2186 probable phosphoglycerate mutase from Rhizobium loti (Mesorhizobium loti) (193 aa), FASTA scores: opt: 423, E(): 7e-21, (41.2% identity in 182 aa overlap); Q9RKV8|SC9G1.08c putative phosphatase from Streptomyces coelicolor (199 aa), FASTA scores: opt: 419, E(): 1.3e-20, (41.1% identity in 185 aa overlap); Q9RDL0|SCC123.14c putative phosphoglycerate mutase from Streptomyces coelicolor (223 aa), FASTA scores: opt: 240, E(): 8.8e-09, (36.9% identity in 168 aa overlap); Q9X194|TM1374 phosphoglycerate mutase from Thermotoga maritima (201 aa), FASTA scores: opt: 218, E(): 2.3e-07, (33.15% identity in 202 aa overlap); etc. But N-terminus also similar to Q9CCH5|ENTC|ML0808 putative isochorismate synthase from Mycobacterium leprae (577 aa), FASTA scores: opt: 346, E(): 2.1e-15, (55.05% identity in 109 aa overlap). N-terminus shows also some similarity with other M. tuberculosis proteins e.g. MTCY427.09c; MTCY20G9.15; MTCY428.28. Equivalent to AAK47652 from Mycobacterium tuberculosis strain CDC1551 (228 aa) but shorter 25 aa. Note that previously known as entD. Q6MWZ7 No intermediary metabolism and respiration 2A6P Q6MWZ7 Q6MWZ7 GO:0004619 5.4.2.1 Q6MWZ7 NC_000962.3 Mycobrowser_v5 CDS 3595713 3595967 . + 0 Rv3219 whiB1 Involved in transcriptional mechanism. Transcriptional regulatory protein WhiB-like WhiB1. Contains [4FE-4S]2+ cluster. Rv3219, (MTCY07D11.07c), len: 84 aa. WhiB1 (alternate gene name: whmE), WhiB-like regulatory protein (see Hutter and Dick, 1999), similar to WhiB paralogue of Streptomyces coelicolor. Equivalent to Q9CCH7|WHIB1|ML0804 putative transcriptional regulator from Mycobacterium leprae (84 aa), FASTA scores: opt: 580, E(): 3.5e-35, (95.25% identity in 84 aa overlap). Highly similar to several e.g. Q9X952|WBLE developmental regulatory protein WhiB-paralog from Streptomyces coelicolor (85 aa), FASTA scores: opt: 477, E(): 9.2e-28, (75.3% identity in 81 aa overlap); Q9AD55|SCP1.95 putative regulatory protein from Streptomyces coelicolor (102 aa), FASTA scores: opt: 383, E(): 6.1e-21, (60.75% identity in 79 aa overlap); Q9K4K8|SC5F8.16c from Streptomyces coelicolor (83 aa), FASTA scores: opt: 346, E(): 2.5e-18, (54.75% identity in 84 aa overlap); etc. P9WF43 No regulatory proteins O05847 P9WF43 P9WF43 NC_000962.3 Mycobrowser_v5 CDS 3611959 3613116 . - 0 Rv3236c Rv3236c Probably involved in transport of undetermined substrate (possibly cations Na/H) across the membrane. Thought to be responsible for the translocation of the substrate across the membrane. Probable conserved integral membrane transport protein Rv3236c, (MTCY20B11.11c), len: 385 aa. Probable conserved integral membrane transport protein, possibly cation (Na/H) transporter, equivalent to Q9CCI5|ML0782 putative transmembrane transport protein from Mycobacterium leprae (385 aa), FASTA scores: opt: 1975, E(): 2.4e-108, (81.55% identity in 385 aa overlap). Highly similar to others e.g. O69958|SC4H2.03c putative transmembrane transport protein from Streptomyces coelicolor (411 aa), FASTA scores: opt: 1226, E(): 1.6e-64, (53.5% identity in 372 aa overlap); Q9XAKO|SC66T3.13c putative transmembrane transport protein from Streptomyces coelicolor (403 aa), FASTA scores: opt: 1198, E(): 6.8e-63, (53.25% identity in 370 aa overlap); Q9RV80|DR1149 putative Na+/H+ antiporter from Deinococcus radiodurans (383 aa), FASTA scores: opt: 1069, E(): 2.3e-55, (47.35% identity in 376 aa overlap); Q9L191|SC10G8.11 putative transmembrane transport protein from Streptomyces coelicolor (446 aa), FASTA scores: opt: 695, E(): 1.9e-33, (38.05% identity in 384 aa overlap); Q9RRW8|DR2367 putative glutathione-regulated potassium-efflux system protein KEFB from Deinococcus radiodurans (575 aa), FASTA scores: opt: 414, E(): 6.2e-17, (30.25% identity in 380 aa overlap); etc. Seems to belong to the CPA2 family. Note that previously known as kefB. L7N665 No cell wall and cell processes Q7D5V5 L7N665 GO:0016021,GO:0015299,GO:0055085,GO:0006812 L7N665 NC_000962.3 Mycobrowser_v5 CDS 3599851 3600699 . + 0 Rv3224 Rv3224 Function unknown; probably involved in cellular metabolism. Possible iron-regulated short-chain dehydrogenase/reductase Rv3224, (MTCY07D11.02c), len: 282 aa. Probable iron-regulated oxidoreductase, possible short-chain dehydrogenase/reductase, highly similar to BAB49551|MLL2413 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (288 aa), FASTA scores: opt: 1053, E(): 6.4e-59, (57.95% identity in 276 aa overlap); Q9AB34|CC0400 short chain dehydrogenase family protein from Caulobacter crescentus (285 aa), FASTA scores: opt: 1051, E(): 8.5e-59, (55.9% identity in 281 aa overlap); and Q9VB10|CG5590 hypothetical protein (similar to the short-chain dehydrogenases/reductases (SDR) family) from Drosophila melanogaster (Fruit fly) (412 aa), FASTA scores: opt: 966, E(): 2.5e-53, (52.15% identity in 278 aa overlap). Similar to various proteins (principaly oxidoreductases) e.g. Q18639|C45B11.3 hypothetical protein (similar to the SDR family) from Caenorhabditis elegans (293 aa), FASTA scores: opt: 921, E(): 1.2e-50, (51.3% identity in 271 aa overlap); Q9HZV5|PA2892 probable short-chain dehydrogenase from Pseudomonas aeruginosa (274 aa), FASTA scores: opt: 847, E(): 5.1e-46, (49.25% identity in 274 aa overlap); Q9I6V0|PA0182 probable short-chain dehydrogenase (similar to the SDR family) from Pseudomonas aeruginosa (250 aa), FASTA scores: opt: 333, E(): 8.3e-14, (29.8% identity in 245 aa overlap); Q9HY98|PA3511 probable short-chain dehydrogenase from Pseudomonas aeruginosa (253 aa), FASTA scores: opt: 330, E(): 1.3e-13, (31.2% identity in 250 aa overlap); etc. Related proteins in Mycobacterium tuberculosis include MTCY02B10.14, MTCY369.14, and MTCY09F9.36. Has ATP/GTP-binding site motif A, (PS00017) near C-terminus. May be belong to the short-chain dehydrogenases/reductases (SDR) family. O05842 No intermediary metabolism and respiration O05842 O05842 GO:0055114,GO:0016491 1.-.-.- NC_000962.3 Mycobrowser_v5 CDS 3607112 3608254 . - 0 Rv3230c Rv3230c Function unknown; probably involved in cellular metabolism. Hypothetical oxidoreductase Rv3230c, (MTCY20B11.05c), len: 380 aa. Putative oxidoreductase, with some similarity to various proteins, especially reductases e.g. Q9HUS4|PA4889 probable oxidoreductase from Pseudomonas aeruginosa (366 aa), FASTA scores: opt: 516, E(): 1.8e-24, (33.8% identity in 367 aa overlap); P95533|TDNB electron transfer protein from Pseudomonas putida (337 aa), FASTA scores: opt: 380, E(): 4e-16, (30.7% identity in 277 aa overlap); BAB34381|ECS0958 NADH oxidoreductase for the HCP from Escherichia coli strain O157:H7 (322 aa), FASTA scores: opt: 369, E(): 1.8e-15, (28.65% identity in 328 aa overlap); Q44253|ATDA5 aniline dioxygenase reductase component from Acinetobacter sp. (336 aa), FASTA scores: opt: 305, E(): 1.6e-11, (27.4% identity in 303 aa overlap); etc. P9WNE9 No intermediary metabolism and respiration O05875 P9WNE9 GO:0005737,GO:0009055,GO:0022900,GO:0046872,GO:0016491,GO:0006810,GO:0051537 P9WNE9 NC_000962.3 Mycobrowser_v5 CDS 3609781 3610371 . - 0 Rv3233c Rv3233c May be involved in synthesis of triacylglycerol Possible triacylglycerol synthase (diacylglycerol acyltransferase) Rv3233c, (MTCY20B11.08c), len: 196 aa. Possible triacylglycerol synthase (See Daniel et al., 2004), similar to C-terminus of Q9RIU8|SCM11.13c hypothetical 47.1 KDA protein from Streptomyces coelicolor (446 aa), FASTA scores: opt: 308, E(): 1.2e-12, (32.0% identity in 200 aa overlap); and several hypothetical M. tuberculosis proteins e.g. O06343|YY80_MYCTU|Rv3480c|MTCY13E12.33c (497 aa), FASTA scores: opt: 248, E(): 9.8e-09, (27.5% identity in 200 aa overlap); MTCY28_26; MTCY493_29; MTCY31_25; MTCY31_25. O05878 No lipid metabolism O05878 O05878 NC_000962.3 Mycobrowser_v5 CDS 3614457 3617603 . - 0 Rv3239c Rv3239c Unknown, but seems involved in efflux system (probably sugar or drug transport). Probable conserved transmembrane transport protein Rv3239c, (MTCY20B11.14c), len: 1048 aa. Probable conserved transmembrane protein, organised in two domains. Domain comprising first ~500 aa residues is similar to various antibiotic resistance and efflux proteins and contains sugar transport proteins signature 1 (PS00216); e.g. Q9RL22|SC5G9.04c putative transmembrane efflux protein from Streptomyces coelicolor (489 aa), FASTA scores: opt: 905, E(): 3.1e-41, (36.95% identity in 482 aa overlap); and O68912|FRNF putative antibiotic antiporter from Streptomyces roseofulvus (517 aa), FASTA scores: opt: 866, E(): 4.1e-39, (37.1% identity in 512 aa overlap). Second part, corresponding to last 550 aa residues, is very similar to Q50733|Rv2565|MTCY9C4.03c hypothetical 62.1 kDa protein from Mycobacterium tuberculosis (583 aa), FASTA scores: E(): 2.1e-28, (36.5% identity in 572 aa overlap). Also equivalent to Rv3728|MTV025.076 putative two-domain membrane protein (similar to sugar transporter family) from Mycobacterium tuberculosis (1065 aa), FASTA scores: opt: 4328, E(): 0, (64.15% identity in 1046 aa overlap); and similar to other Mycobacterium tuberculosis proteins: MTCY3G12.01, E(): 6.3e-32; MTCY98.02c, E(): 6.3e-32; MTCY9C4.03c, E(): 1.5e-26; MTCY369.27c, E(): 2.5e-26. Equivalent to AAK47679 Drug transporter from Mycobacterium tuberculosis strain CDC1551 (1065 aa) but shorter 20 aa. Contains cyclic nucleotide-binding domain signature 2 (PS00889). Probably member of major facilitator superfamily (MFS). O05884 No cell wall and cell processes O05884 O05884 GO:0016021,GO:0004622,GO:0046470,GO:0046677,GO:0015904,GO:0015520,GO:0055085,GO:0005789 O05884 NC_000962.3 Mycobrowser_v5 CDS 3624910 3626613 . - 0 Rv3245c mtrB Sensor part of a two component regulatory system. Two component sensory transduction histidine kinase MtrB Rv3245c, (MTCY20B11.20c), len: 567 aa. MtrB, sensor-like histidine kinase (see citations below), equivalent to Q9CCJ1|MTRB or ML0774 putative two-component system sensor kinase from Mycobacterium leprae (562 aa), FASTA scores: opt: 3208, E(): 7.4e-173, (88.7% identity in 566 aa overlap). Also similar to others e.g. Q9KYW9|SCE33.14c putative two-component system histidine kinase from Streptomyces coelicolor (688 aa), FASTA scores: opt: 1355, E(): 1.1e-68, (48.95% identity in 515 aa overlap); etc. Relatives in Mycobacterium tuberculosis are: MTCY369.03, E(): 1.5e-22; MTCY20G9.16, E(): 1.9e-17. Similar to other prokaryotic sensory transduction histidine kinases. P9WGK9 No regulatory proteins Q50496 P9WGK9 GO:0016021,GO:0018106,GO:0005886,GO:0007165,GO:0000155,GO:0000160,GO:0005524 2.7.13.3 P9WGK9 NC_000962.3 Mycobrowser_v5 CDS 3626663 3627349 . - 0 Rv3246c mtrA Transcriptional activator part of a two component regulatory system. Two component sensory transduction transcriptional regulatory protein MtrA Rv3246c, (MTCY20B11.21c), len: 228 aa. MtrA, transcriptional activator, response regulator (see citations below), equivalent to Q9CCJ2|MTRA|ML0773 putative two-component response regulator from Mycobacterium leprae (228 aa), FASTA scores: opt: 1458, E(): 1.4e-85, (98.7% identity in 228 aa overlap). Also highly similar to others e.g. Q9F9J5|SCRA putative response regulator from Streptomyces coelicolor (228 aa), FASTA scores: opt: 1141, E(): 1.9e-65, (74.9% identity in 227 aa overlap); Q9KYW8|SCE33.15c putative two-component system response regulator from Streptomyces coelicolor (229 aa), FASTA scores: opt: 1141, E(): 1.9e-65, (74.9% identity in 227 aa overlap); Q9F868|REGX3 response regulator REGX3 from Mycobacterium smegmatis (228 aa), FASTA scores: opt: 730, E(): 2.3e-39, (50.90% identity in 222 aa overlap); etc. Relatives in Mycobacterium tuberculosis are: U01971|MTU01971_1; Q11156|RGX3_MYCTU; MTCY20G9.17, E(): 0; MTCY31.31c, E(): 3.4e-29; MTCY369.02, E(): 5.7e-28. Similar to bacterial regulatory proteins involved in signal transduction. The N-terminal region is similar to that of other regulatory components of sensory transduction systems. Experiments showed mtrA is differentially expressed in virulent and avirulent strains during growth in macrophages. P9WGM7 No regulatory proteins 2GWR,3NHZ P0A5Z4 P9WGM7 GO:0006355,GO:0006350,GO:0000156,GO:0000160,GO:0003677 P9WGM7 NC_000962.3 Mycobrowser_v5 CDS 3643177 3644838 . + 0 Rv3263 Rv3263 Causes DNA methylation. Probable DNA methylase (modification methylase) (methyltransferase) Rv3263, (MTCY71.03), len: 553 aa. Probable DNA methylase, equivalent to Q9CCK4|ML0756 probable DNA methylase from Mycobacterium leprae (555 aa), FASTA scores: opt: 2980, E(): 2.1e-184, (81.9% identity in 541 aa overlap). Also similar to others e.g. P25240|MT57_ECOLI|ECO57IM modification methylase from Escherichia coli (544 aa), FASTA scores: opt: 595, E(): 1e-30, (30.35% identity in 507 aa overlap); P25201|MTA1_ACICA|ACCIM modification methylase ACCI from Acinetobacter calcoaceticus (540 aa), FASTA scores: opt: 366, E(): 5.7e-16, (23.35% identity in 467 aa overlap); Q56752|M-ACCI ACCI methylase from Bergeyella zoohelcum (541 aa), FASTA scores: opt: 365, E(): 6.6e-16, (22.95% identity in 466 aa overlap); etc. Contains PS00092 N-6 Adenine-specific DNA methylases signature. Alternative start site at aa 25. P96868 No information pathways P96868 P96868 GO:0006306,GO:0008170,GO:0009007,GO:0003677 2.1.1.72 P96868 NC_000962.3 Mycobrowser_v5 CDS 3628160 3629647 . - 0 Rv3248c sahH Thioester hydrolase which acting on ether bounds. Could be involved in methionine and selenoamino acid metabolisms. Also involved in activated methyl. Cycle adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine [catalytic activity: S-adenosyl-L-homocysteine + H(2)O = adenosine + L-homocysteine]. Probable adenosylhomocysteinase SahH (S-adenosyl-L-homocysteine hydrolase) (adohcyase) Rv3248c, (MTCY20B11.23c), len: 495 aa. Probable sahH, adenosylhomocysteinase, equivalent to Q9CCJ4|SAHH|ML0771 putative S-adenosyl-L-homocysteine hydrolase from Mycobacterium leprae (492 aa), FASTA scores: opt: 3019, E(): 1.3e-177, (91.4% identity in 489 aa overlap). Also highly similar to other adenosylhomocysteinases e.g. Q9KZM1|SAHH from Streptomyces coelicolor (485 aa), FASTA scores: opt: 2258, E(): 5.7e-131, (70.0% identity in 483 aa overlap); P51540|SAHH_TRIVA from Trichomonas vaginalis (486 aa), FASTA scores: opt: 2005, E(): 1.8e-115, (62.05% identity in 477 aa overlap); P35007|SAHH_CATRO from Catharanthus roseus (Rosy periwinkle) (Madagascar periwinkle) (485 aa), FASTA scores: opt: 1941, E(): 1.5e-111, (60.15% identity in 492 aa overlap); etc. Has S-adenosyl-L-homocysteine hydrolase signature (PS00739). Belongs to the adenosylhomocysteinase family. P9WGV3 No intermediary metabolism and respiration 2ZIZ,2ZJ0,2ZJ1,3CE6,3DHY P60176 P9WGV3 GO:0005737,GO:0006730,GO:0004013 3.3.1.1 P9WGV3 NC_000962.3 Mycobrowser_v5 CDS 3630738 3631988 . - 0 Rv3252c alkB Thought to be involved in fatty acid metabolism. Generates octanol and oxidized rubredoxin from octane and reduced rubredoxin. Also hydroxylates fatty acids in the omega-position [catalytic activity: octane + reduced rubredoxin + (O)2 = 1-octanol + oxidized rubredoxin + H(2)O]. Probable transmembrane alkane 1-monooxygenase AlkB (alkane 1-hydroxylase) (lauric acid omega-hydroxylase) (omega-hydroxylase) (fatty acid omega-hydroxylase) (alkane hydroxylase-rubredoxin) Rv3252c, (MTCY20B11.27c), len: 416 aa. Probable alkB, transmembrane alkane-1-monooxygenase, highly similar to many (see Marin et al., 2001) e.g. Q9AE68|ALKB2 from Rhodococcus erythropolis (408 aa), FASTA scores: opt: 2018, E(): 9.6e-122, (68.6% identity in 415 aa overlap); Q9AFD5|ALKB from Nocardioides sp. CF8 (483 aa), FASTA scores: opt: 1485, E(): 1.4e-87, (56.55% identity in 405 aa overlap); Q9XAU0|ALKB1 from Rhodococcus erythropolis (391 aa), FASTA scores: opt: 1400, E(): 3.3e-82, (62.6% identity in 396 aa overlap); Q9XBM1|ALKB from Prauserella rugosa (490 aa), FASTA scores: opt: 1266, E(): 1.5e-73, (57.55% identity in 410 aa overlap); CAC40954|ALKB4 from Rhodococcus erythropolis (386 aa), FASTA scores: opt: 1190, E(): 9.1e-69, (54.3% identity in 383 aa overlap); etc. O05895 No lipid metabolism O05895 O05895 GO:0016021,GO:0006629,GO:0055114,GO:0018685 1.14.15.3 NC_000962.3 Mycobrowser_v5 CDS 3635041 3636267 . - 0 Rv3255c manA This enzyme converts D-mannose 6-phosphate to D-fructose 6-phosphate [catalytic activity: D-mannose 6-phosphate = D-fructose 6-phosphate]. Probable mannose-6-phosphate isomerase ManA (phosphomannose isomerase) (phosphomannoisomerase) (PMI) (phosphohexoisomerase) (phosphohexomutase) Rv3255c, (MTCY20B11.30c), len: 408 aa. Probable manA, mannose-6-phosphate isomerase, equivalent to Q9CCJ5|MANA|ML0765 putative mannose-6-phosphate isomerase from Mycobacterium leprae (410 aa), FASTA scores: opt: 2271, E(): 1.6e-133, (84.45% identity in 411 aa overlap). Also similar to many others e.g. Q9KZL9|MANA from Streptomyces coelicolor (383 aa), FASTA scores: opt: 946, E(): 2.4e-51, (44.4% identity in 403 aa overlap); Q9KV87|VC0269 from Vibrio cholerae (399 aa), FASTA scores: opt: 726, E(): 1.1e-37, (34.15% identity in 404 aa overlap); Q9CMJ5|PMI|PM0829 from Pasteurella multocida (400 aa), FASTA scores: opt: 640, E(): 2.4e-32, (32.5% identity in 391 aa overlap); etc. Similar to family 1 of mannose-6-phosphate isomerases. O05898 No intermediary metabolism and respiration O05898 O05898 GO:0004476,GO:0008270,GO:0005975 5.3.1.8 O05898 NC_000962.3 Mycobrowser_v5 CDS 3640543 3641538 . + 0 Rv3261 fbiA Required for coenzyme F420 production: involved in the conversion of FO into F420. Probable F420 biosynthesis protein FbiA Rv3261, (MTCY71.01), len: 331 aa. Probable fbiA, F420 biosynthesis protein, equivalent to FBIA F420 biosynthesis protein fbiA from Mycobacterium bovis BCG (see citations below). Also equivalent, but shorter 46 aa, to Q9CCK1|ML0759 hypothetical protein from Mycobacterium leprae (379 aa), FASTA scores: opt: 1855, E(): 3.9e-110, (79.3% identity in 333 aa overlap). Also similar to others e.g. Q9KZK9|SCE34.17 hypothetical 33.6 KDA protein from Streptomyces coelicolor (319 aa), FASTA scores: opt: 1151, E(): 1.2e-65, (55.1% identity in 332 aa overlap); O29345|AF0917 conserved hypothetical protein from Archaeoglobus fulgidus (296 aa), FASTA scores: opt: 469, E(): 1.7e-22, (31.15% identity in 302 aa overlap); Q58653|MJ1256 hypothetical protein from Methanococcus jannaschii (311 aa), FASTA scores: opt: 436, E(): 2.2e-20, (27.35% identity in 274 aa overlap); etc. P9WP81 No intermediary metabolism and respiration P96866 P9WP81 GO:0000287,GO:0016773,GO:0016780,GO:0009108 2.7.8.- P9WP81 NC_000962.3 Mycobrowser_v5 CDS 3644898 3645977 . - 0 Rv3264c manB Involved in GDP-mannose biosynthesis and biosynthesis of nucleotide-activated glycero-manno-heptose (D-alpha-D pathway): generates GDP-mannose and phosphate from GTP and alpha-D-mannose 1-phosphate. MANB product is needed for all mannosyl glycolipids and polysaccharides which, like rhamnosyl residues, are an important part of the mycobacterium envelope [catalytic activity: alpha-D-mannose 1-phosphate + GTP = GDP-mannose + phosphate]. D-alpha-D-mannose-1-phosphate guanylyltransferase ManB (D-alpha-D-heptose-1-phosphate guanylyltransferase) Rv3264c, (MTCY71.04c), len: 359 aa. ManB (alternate gene name: hddC), D-alpha-D-mannose-1-phosphate guanylyltransferase (see citations below), equivalent to Q9CCK6|RMLA2|ML0753 putative sugar-phosphate nucleotidyl transferase from Mycobacterium leprae (358 aa), FASTA scores: opt: 2075, E(): 2.7e-115, (86.9% identity in 359 aa overlap). Also similar to others e.g. Q9KZK6|SCE34.20c putative nucleotide phosphorylase from Streptomyces coelicolor (360 aa), FASTA scores: opt: 1314, E(): 2.2e-70, (57.0% identity in 358 aa overlap); Q9KZP4|SC1A8A.08 putative mannose-1-phosphate guanyltransferase from Streptomyces coelicolor (831 aa), FASTA scores: opt: 699, E(): 8.6e-34, (34.45% identity in 354 aa overlap) (only similarity in N-terminus for this one); P74589|SLL1496 mannose-1-phosphate guanyltransferase from Synechocystis sp. strain PCC 6803 (843 aa), FASTA scores: opt: 692, E(): 2.3e-33, (35.1% identity in 342 aa overlap) (only similarity in N-terminus for this one too); BAB59222|TVG0079558 mannose-1-phosphate guanyltransferase from Thermoplasma volcanium (359 aa), FASTA scores: opt: 664, E(): 5.2e-32, (34.6% identity in 338 aa overlap); Q9ZTW5|GMP GDP-mannose pyrophosphorylase from Solanum tuberosum (Potato) (361 aa), FASTA scores: opt: 636, E(): 2.3e-30, (34.65% identity in 361 aa overlap); etc. Belongs to family 2 of mannose-6-phosphate isomerases. Note that previously known as rmlA2. L7N6A5 No cell wall and cell processes Q7D5T3 L7N6A5 GO:0004475,GO:0009058 2.7.7.13 EMB L7N6A5 NC_000962.3 Mycobrowser_v5 CDS 3650234 3650515 . + 0 Rv3269 Rv3269 Function unknown. May be involved in a chaperoning process. Conserved protein Rv3269, (MTCY71.09), len: 93 aa. Conserved protein, similar to many Mycobacterium proteins and chaperonins/heat shock proteins e.g. Q9CCL0|ML0748 hypothetical protein from Mycobacterium leprae (92 aa), FASTA scores: opt: 427, E(): 6.8e-21, (73.65% identity in 91 aa overlap); Q10865|Rv1993c|MT2049|MTCY39.26c hypothetical protein from Mycobacterium tuberculosis (90 aa), FASTA scores: opt: 313, E(): 1.2e-13, (60.7% identity in 84 aa overlap); P71542|Y968_MYCTU|Rv0968|MTCY10D7.06c (98 aa), FASTA scores: opt: 294, E(): 2.2e-12, (55.1% identity in 98 aa overlap); Q50827|MOPA|GROEL|CH60_MYCVA chaperonin (protein CPN60) from Mycobacterium vaccae (120 aa), FASTA scores: opt: 107, E(): 2.1, (39.5% identity in 81 aa overlap); Q9AEB3|HSP65 heat shock protein (fragment) from Mycobacterium gadium (122 aa), FASTA scores: opt: 102, E(): 4.4, (38.25% identity in 81 aa overlap); Q49374|CH60_MYCGN|MOPA|GROEL chaperonin (protein CPN60) from Mycobacterium genavense (120 aa), FASTA scores: opt: 99, E(): 6.8, (40.25% identity in 82 aa overlap); etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P96874 No virulence, detoxification, adaptation P96874 P96874 NC_000962.3 Mycobrowser_v5 CDS 3653448 3654632 . + 0 Rv3272 Rv3272 Function unknown Conserved hypothetical protein Rv3272, (MTCY71.12), len: 394 aa. Conserved hypothetical protein, similar to various proteins e.g. Q9I672|PA0446 hypothetical protein from Pseudomonas aeruginosa (407 aa), FASTA scores: opt: 643, E(): 6.8e-32, (33.15% identity in 389 aa overlap); Q9RJU8|SCF41.21 putative racemase from Streptomyces coelicolor (403 aa), FASTA scores: opt: 541, E(): 1.1e-25, (31.95% identity in 385 aa overlap); O87838|SC8A6.04c putative transferase from Streptomyces coelicolor (410 aa), FASTA scores: opt: 539, E(): 1.5e-25, (29.95% identity in 395 aa overlap); Q9I563|PA0882 from Pseudomonas aeruginosa (400 aa), FASTA scores: opt: 530, E(): 5.2e-25, (28.8% identity in 396 aa overlap); BAB60328|TVG1215416 L-carnitine dehydratase from Thermoplasma volcanium (399 aa), FASTA scores: opt: 529, E(): 6e-25, (32.9% identity in 383 aa overlap); etc. C-terminus is similar to Q49678|U00012_27|B1308_C3_195 from Mycobacterium leprae (130 aa) (60.0% identity in 115 aa overlap). Also partially similar to MTCY359_7 from M. tuberculosis (778 aa) (29.9% identity in 388 aa overlap). P96877 No conserved hypotheticals P96877 P96877 GO:0008152,GO:0003824 P96877 NC_000962.3 Mycobrowser_v5 CDS 3654637 3656931 . + 0 Rv3273 Rv3273 Generates CO(2) and H(2)O from H(2)co(3), and possibly involved in transport of sulfate across the membrane. Probable transmembrane carbonic anhydrase (carbonate dehydratase) (carbonic dehydratase) Rv3273, (MTCY71.13), len: 764 aa. Probable transmembrane protein (N-terminal part is hydrophobic) with probable carbonic anhydrase activity (in C-terminal part). Possibly involved in transport of sulfate. Equivalent to Q9CBA3|ML2279 putative transmembrane transport protein from Mycobacterium leprae (496 aa), FASTA scores: opt: 1637, E(): 1.8e-89, (59.15% identity in 487 aa overlap). Similar to various proteins (principally sulfate transporters) e.g. Q9X927|SCH5.25 putative integral membrane protein from Streptomyces coelicolor (830 aa), FASTA scores: opt: 1325, E(): 8e-71, (40.85% identity in 788 aa overlap); Q9I729|PA0103 probable sulfate transporter from Pseudomonas aeruginosa (523 aa), FASTA scores: opt: 1015, E(): 1.3e-52, (39.95% identity in 488 aa overlap); Q9KN88|VCA0077 sulfate permease family protein from Vibrio cholerae (553 aa), FASTA scores: opt: 629, E(): 9.6e-30, (30.95% identity in 423 aa overlap); etc. C-terminal part (aa 550-764) shows similarity to carbonic anhydrase e.g. P27134|CYNT_SYNP7 carbonic anhydrase (272 aa), FASTA scores: opt: 350, E(): 8.1e-15, (33.8% identity in 201 aa overlap). Contains PS00704 Prokaryotic-type carbonic anhydrases signature 1. Seems to belong to the SulP family. P96878 No intermediary metabolism and respiration P96878 P96878 GO:0004089,GO:0016021,GO:0055085,GO:0005215,GO:0008270,GO:0015976 4.2.1.1 P96878 NC_000962.3 Mycobrowser_v5 CDS 4145614 4146891 . - 0 Rv3703c Rv3703c Function unknown Conserved hypothetical protein Rv3703c, (MTV025.051c), len: 425 aa. Conserved hypothetical protein, similar to other hypothetical proteins e.g. Q9RD00|SCM1.44 from Streptomyces coelicolor (446 aa), FASTA scores: opt: 1480, E(): 1.4e-85, (53.9% identity in 421 aa overlap); P72841|SLR1303 from Synechocystis sp. strain PCC 6803 (410 aa), FASTA scores: opt: 533, E(): 4.5e-26, (36.6% identity in 429 aa overlap); Q9KYH7|SCC61A.16 from Streptomyces coelicolor (256 aa), FASTA scores: opt: 266, E(): 1.9e-09, (32.25% identity in 248 aa overlap); etc. Also similar to P95060|Rv0712|MTCY210.31 hypothetical 32.7 KDA protein from Mycobacterium tuberculosis (299 aa), FASTA scores: opt: 243, E(): 5.9e-08, (30.6% identity in 304 aa overlap). O69671 No conserved hypotheticals O69671 O69671 O69671 NC_000962.3 Mycobrowser_v5 CDS 3658114 3658638 . - 0 Rv3275c purE Involved in purine biosynthesis (sixth step). This subunit can alone transform air to CAIR, but in association with PURK, which possesses an ATPase activity, an enzyme complex is produced which is capable of converting air to CAIR efficiently under physiological condition [catalytic activity: 1-(5-phosphoribosyl)-5-amino-4-imidazole-carboxylate = 1-(5-phosphoribosyl)-5-aminoimidazole + CO(2)]. Probable phosphoribosylaminoimidazole carboxylase catalytic subunit PurE (air carboxylase) (AIRC) Rv3275c, (MTCY71.15c, PUR6), len: 174 aa. Probable purE, phosphoribosylaminoimidazole carboxylase catalytic subunit, equivalent to P46702|PUR6_MYCLE|pure|ML0736|B1308_F3_98 from Mycobacterium leprae (171 aa), FASTA scores: opt: 878, E(): 1.5e-43, (81.55% identity in 168 aa overlap). Also similar to others e.g. Q9AXD0|AIRC from Nicotiana tabacum (Common tobacco) (623 aa), FASTA scores: opt: 712, E(): 1.4e-33, (69.35% identity in 160 aa overlap) (similarity in C-terminal part for this one); Q44679|PUR6_CORAM from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (177 aa), FASTA scores: opt: 651, E(): 1.5e-30, (68.25% identity in 148 aa overlap); Q55498|PUR6_SYNY3|pure|SLL0901 from Synechocystis sp. strain PCC 6803 (176 aa), FASTA scores: opt: 639, E(): 7.1e-30, (60.5% identity in 167 aa overlap); etc. P9WHM1 No intermediary metabolism and respiration 3LP6 P96880 P9WHM1 GO:0004638,GO:0006189 4.1.1.21 P9WHM1 NC_000962.3 Mycobrowser_v5 CDS 3682110 3683024 . - 0 Rv3298c lpqC Function unknown; lipolytic enzyme involved in cellular metabolism. Possible esterase lipoprotein LpqC Rv3298c, (MTCY71.38c), len: 304 aa. Possible lpqC, esterase lipoprotein, equivalent to Q9CCL5|LPQC|ML0715 putative secreted hydrolase from Mycobacterium leprae (304 aa), FASTA scores: opt: 1543, E(): 1.3e-87, (71.6% identity in 303 aa overlap); and Q49658|B1308_F2_43 tubulin family protein from Mycobacterium leprae (302 aa), FASTA scores: opt: 1541, E(): 1.7e-87, (72.0% identity in 300 aa overlap). Also similar to Q9I5Z3|PA0543 hypothetical protein from Pseudomonas aeruginosa (322 aa), FASTA scores: opt: 439, E(): 8.9e-20, (32.3% identity in 319 aa overlap); Q9F2K9|SCH63.19c putative secreted protein from Streptomyces coelicolor (348 aa), FASTA scores: opt: 394, E(): 5.5e-17, (30.25% identity in 334 aa overlap); etc. And similar to O86367|LPQP|Rv0671|MTCI376.03c from Mycobacterium tuberculosis strain H37Rv (280 aa), FASTA scores: opt: 519, E(): 9.8e-25, (39.25% identity in 275 aa overlap). Probably lipoprotein, esterase membrane-bound, with 18 aa signal sequence as it contains appropriately positioned (PS00013) Prokaryotic membrane lipoprotein lipid attachment site. P96903 No cell wall and cell processes P96903 GO:0016787 3.1.-.- P96903 NC_000962.3 Mycobrowser_v5 CDS 3658635 3659924 . - 0 Rv3276c purK Involved in purine biosynthesis (sixth step). Possesses an ATPase activity that is dependent on the presence of air (aminoimidazole ribonucleotide). The association of PURK and pure produces an enzyme complex capable of converting air to CAIR efficiently under physiological condition [catalytic activity: 1-(5-phosphoribosyl)-5-amino-4-imidazole-carboxylate = 1-(5-phosphoribosyl)-5-aminoimidazole + CO(2)]. Probable phosphoribosylaminoimidazole carboxylase ATPase subunit PurK (air carboxylase) (AIRC) Rv3276c, (MTCY71.16c), len: 429 aa. Probable purK, phosphoribosylaminoimidazole carboxylase ATPase subunit , equivalent to P46701|PURK_MYCLE|ML0735|B1308_F1_32 phosphoribosylaminoimidazole carboxylase ATPase subunit from Mycobacterium leprae (439 aa), FASTA scores: opt: 2168, E(): 2.3e-123, (76.15% identity in 444 aa overlap). Also similar to others e.g. Q44678|PURK_CORAM from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (413 aa), FASTA scores: opt: 1179, E(): 9.1e-64, (48.35% identity in 389 aa overlap); Q9KZ85|PURK from Streptomyces coelicolor (368 aa), FASTA scores: opt: 1150, E(): 4.7e-62, (55.35% identity in 345 aa overlap); Q54975|PURK_SYNP7 from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (395 aa), FASTA scores: opt: 772, E(): 3e-39, (38.1% identity in 383 aa overlap); etc. Belongs to the PurK / PurT family. P9WHL9 No intermediary metabolism and respiration P65898 P9WHL9 GO:0005524,GO:0004638,GO:0006189 4.1.1.21 P9WHL9 NC_000962.3 Mycobrowser_v5 CDS 3661212 3662012 . - 0 Rv3279c birA BIRA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins [catalytic activity: ATP + biotin + APO-[acetyl-CoA:carbon-dioxide ligase (ADP forming)] = AMP + pyrophosphate + [acetyl-CoA:carbon-dioxide ligase (ADP forming)]]. Possible bifunctional protein BirA: biotin operon repressor + biotin--[acetyl-CoA-carboxylase] synthetase (biotin--protein ligase) Rv3279c, (MTCY71.19c), len: 266 aa. Possible birA, bifunctional protein: biotin operon repressor and biotin--[acetyl-CoA-carboxylase] synthetase, equivalent to Q9CCL3|BIRA|ML0732 biotin APO-protein ligase from Mycobacterium leprae (274 aa), FASTA scores: opt: 1189, E(): 2.3e-66, (71.2% identity in 271 aa overlap). But as it lacks a BirA h-t-h domain at N-terminus, may simply be biotin apo-protein ligase. Also similar to others e.g. Q9CNX6|BIRA|PM0296 from Pasteurella multocida (312 aa), FASTA scores: opt: 347, E(): 2.7e-14, (32.95% identity in 270 aa overlap); Q9HWC0|BIRA|PA4280 from Pseudomonas aeruginosa (312 aa), FASTA scores: opt: 335, E(): 1.5e-13, (34.2% identity in 272 aa overlap); Q9A6Z0|CC1936 from Caulobacter crescentus (250 aa), FASTA scores: opt: 332, E(): 1.9e-13, (33.6% identity in 238 aa overlap); P06709|BIRA_ECOLI (321 aa), FASTA scores: opt: 314, E(): 3.1e-12, (34.15% identity in 249 aa overlap); etc. Similar with other bacterial BIRA and with eukaryotic biotin APO-protein ligase. I6YFP0 No intermediary metabolism and respiration 2CGH,3L1A,3L2Z,3RUX P96884 I6YFP0 GO:0006464,GO:0004077 6.3.4.15 I6YFP0 NC_000962.3 Mycobrowser_v5 CDS 3664219 3664887 . + 0 Rv3282 Rv3282 Function unknown Conserved hypothetical protein Rv3282, (MTCY71.22), len: 222 aa. Conserved hypothetical protein, equivalent to Q49670|ML0729 1308R (hypothetical protein ML0729) from Mycobacterium leprae (213 aa), FASTA scores: opt: 945, E(): 5.5e-54, (68.55% identity in 213 aa overlap). Also similar to Q9EWV6|2SCK31.18 conserved hypothetical protein from Streptomyces coelicolor (206 aa), FASTA scores: opt: 459, E(): 1.3e-22, (47.35% identity in 209 aa overlap); P74331|MAF or SLL0905 MAF protein from Synechocystis sp. strain PCC 6803 (195 aa), FASTA scores: opt: 401, E(): 6.9e-19, (43.0% identity in 207 aa overlap); and shows weak similarity with various proteins e.g. Q9BUL6 acetylserotonin O-methyltransferase-like from Homo sapiens (Human) (621 aa), FASTA scores: opt: 282, E(): 8.9e-11, (31.6% identity in 193 aa overlap); O95671|ASMTL ASMTL protein from Homo sapiens (Human) (629 aa), FASTA scores: opt: 282, E(): 9e-11, (31.6% identity in 193 aa overlap); BAB51136|MLR4491 MAF protein from Rhizobium loti (Mesorhizobium loti) (199 aa), FASTA scores: opt: 269, E(): 2.3e-10, (29.3% identity in 198 aa overlap); etc. P9WK27 No conserved hypotheticals P96887 P9WK27 GO:0005737 P9WK27 NC_000962.3 Mycobrowser_v5 CDS 3708438 3709052 . - 0 Rv3322c Rv3322c Could cause methylation. Possible methyltransferase Rv3322c, (MTV016.22c), len: 204 aa. Conserved hypothetical protein, showing weak similarity to proteins including several methyltransferases e.g. Q9X9V1|ORF8 putative methyltransferase from Streptomyces coelicolor (208 aa), FASTA scores: opt: 193, E(): 1e-05, (36.35% identity in 132 aa overlap); and Q9XA90|SCF43A.25c putative methyltransferase from Streptomyces coelicolor (215 aa), FASTA scores: opt: 161, E(): 0.0014, (32.05% identity in 131 aa overlap); P74712|SLR1183 hypothetical 21.3 KDA protein from Synechocystis sp. strain PCC 6803 (194 aa), FASTA scores: opt: 155, E(): 0.0032, (27.35% identity in 150 aa overlap); Q9ABW8|CC0102 rRNA methyltransferase RSMB from Caulobacter crescentus (429 aa), FASTA scores: opt: 148, E(): 0.018, (31.5% identity in 162 aa overlap); etc. Also highly similar to O05796|Rv3120|MTCY164.30 hypothetical 21.8 KDA protein from Mycobacterium tuberculosis (200 aa), FASTA scores: opt: 691, E(): 1.2e-38, (56.5% identity in 200 aa overlap); and shows weak similarity to O69667|Rv3699|MTV025.047 putative methyltransferase from Mycobacterium tuberculosis (233 aa), FASTA scores: opt: 155, E(): 0.0037, (29.15% identity in 168 aa overlap). L7N687 No intermediary metabolism and respiration L7N687 GO:0008168 2.1.1.- L7N687 NC_000962.3 Mycobrowser_v5 CDS 3668169 3668954 . - 0 Rv3286c sigF The sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released. Thought to be involved in survival and proliferation in lung granulomas during infection. Thought to be involved in virulence and persistence processes. Modulates expression of the 16 KDa alpha-crystallin homologue/Rv2031c. Negatively regulated by Rv3287c|RSBW|USFX. Alternative RNA polymerase sigma factor SigF Rv3286c, (MTCY71.26), len: 261 aa. SigF, stress response/stationary phase RNA polymerase sigma factor (see citations below), similar to several Streptomyces RNA polymerase sigma factors e.g. Q9RPC8|sigh from Streptomyces coelicolor A3(2) (354 aa), FASTA scores: opt: 869, E(): 1.1e-45, (51.15% identity in 258 aa overlap); Q9RIT0|SIG1 from Streptomyces coelicolor (361 aa), FASTA scores: opt: 869, E(): 1.1e-45, (51.15% identity in 258 aa overlap); Q9ADM4|2SC10A7.38c from Streptomyces coelicolor (318 aa), FASTA scores: opt: 776, E(): 4.6e-40, (48.75% identity in 240 aa overlap); P37971|RPOF_STRCO|SIGF|RPOX|2SCD60.01c from Streptomyces coelicolor (287 aa), FASTA scores: opt: 717, E(): 1.6e-36, (44.5% identity in 245 aa overlap); P37970|RPOF_STRAU|SIGF|RPOX from Streptomyces aureofaciens (297 aa); etc. Contains possible helix-turn-helix motif at aa 229-250 (+7.38 SD). Similar to the sigma-70 factor family. Seems expressed in stationary phase and under stress conditions in vitro (see citations below). P9WGI3 No information pathways Q7D5S2 P9WGI3 GO:0016987,GO:0003700,GO:0006352,GO:0006355 P9WGI3 NC_000962.3 Mycobrowser_v5 CDS 3670445 3671794 . - 0 Rv3290c lat Possibly involved in L-alpha-aminoadipic acid (L-AAA) biosynthesis. Catalyzes the transfer of the terminal amino group of L-lysine or L-ornithine to alpha-ketoglutarate [catalytic activity: L-lysine + 2-oxoglutarate = 2-aminoadipate 6-semialdehyde + L-glutamate]. Probable L-lysine-epsilon aminotransferase Lat (L-lysine aminotransferase) (lysine 6-aminotransferase) Rv3290c, (MTCY71.30), len: 449 aa. Probable lat, lysine-epsilon aminotransferase, similar to Q05174|LAT_NOCLA from Nocardia lactamdurans (450 aa), FASTA scores: opt: 1702, E(): 1.1e-99, (60.35% identity in 439 aa overlap); and Q01767|Q53823|LAT_STRCL from Streptomyces clavuligerus (457 aa), FASTA scores: opt: 1676, E(): 4.9e-98, (60.15% identity in 434 aa overlap). Also some similarity to 4-aminobutyrate aminotransferase proteins (gamma-amino-N-butyrate transaminases). Belongs to class-III of pyridoxal-phosphate-dependent aminotransferases. Cofactor: pyridoxal phosphate. P9WQ77 No intermediary metabolism and respiration 2CIN,2CJD,2CJG,2CJH,2JJE,2JJF,2JJG,2JJH P63509 P9WQ77 GO:0017000,GO:0030170,GO:0045484 2.6.1.36 P9WQ77 NC_000962.3 Mycobrowser_v5 CDS 3673602 3675086 . + 0 Rv3293 pcd Involved in L-alpha-aminoadipic acid (L-AAA) biosynthesis (in the second step; the first step is promoted by lat enzyme. Probable piperideine-6-carboxilic acid dehydrogenase Pcd (piperideine-6-carboxylate dehydrogenase) Rv3293, (MTCY71.33), len: 494 aa. Probable pcd, piperideine-6-carboxylic acid dehydrogenase, highly similar to others e.g. O85725|PCD semialdehyde dehydrogenase from Streptomyces clavuligerus (512 aa), FASTA scores: opt: 2214, E(): 6.7e-121, (68.75% identity in 496 aa overlap) (see Alexander & Jensen 1998); Q9I4U7|PA1027 probable aldehyde dehydrogenase from Pseudomonas aeruginosa (529 aa), FASTA scores: opt: 1984, E(): 1.4e-107, (64.5% identity in 493 aa overlap); BAB49892|MLL2867 aldehyde dehydrogenase from Rhizobium loti (Mesorhizobium loti) (504 aa), FASTA scores: opt: 1964, E(): 2e-106, (62.8% identity in 476 aa overlap); Q9A8Y1|CC1216 aldehyde dehydrogenase from Caulobacter crescentus (507 aa), FASTA scores: opt: 1909, E(): 3.1e-103, (59.95% identity in 497 aa overlap); O54199|PCD piperideine-6-carboxilic acid dehydrogenase from Streptomyces clavuligerus (496 aa), FASTA scores: opt: 1748, E(): 6.4e-94, (60.6% identity in 467 aa overlap); and Q9F1U8|PCD piperideine-6-carboxylate dehydrogenase from 'Flavobacterium' lutescens (510 aa), FASTA scores: opt: 1656, E(): 1.4e-88, (54.05% identity in 481 aa overlap) (see Fujii et al., 2000); etc. Contains PS00687 Aldehyde dehydrogenases glutamic acid active site. Note that ORF Rv3290c seems to encoded the putative lat enzyme. Note that previously known as aldB. L7N650 No intermediary metabolism and respiration Q7D5R7 L7N650 GO:0016491,GO:0055114 1.5.-.- L7N650 NC_000962.3 Mycobrowser_v5 CDS 3676066 3676731 . + 0 Rv3295 Rv3295 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably TetR-family) Rv3295, (MTCY71.35), len: 221 aa. Probable transcriptional regulator TetR-family, equivalent to Q9CCL4|ML0717 putative TetR-family transcriptional regulator from Mycobacterium leprae (223 aa), FASTA scores: opt: 1260, E(): 7.2e-75, (85.45% identity in 220 aa overlap). Also highly similar to other streptomyces regulators e.g. Q9RD77|SCF43.11 from Streptomyces coelicolor (205 aa), FASTA scores: opt: 442, E(): 9.8e-22, (38.6% identity in 202 aa overlap); Q9RKY8|SC6D7.09 from Streptomyces coelicolor (220 aa), FASTA scores: opt: 215, E(): 5.9e-07, (31.85% identity in 135 aa overlap); Q9L0U5|SCD35.06 from Streptomyces coelicolor (240 aa), FASTA scores: opt: 214, E(): 7.4e-07, (28.2% identity in 156 aa overlap); etc. Similar to the TetR/AcrR family of transcriptional regulators. Contains potential helix-turn-helix motif at aa 33-54 (+4.42 SD). P96900 No regulatory proteins P96900 P96900 GO:0006350,GO:0003700,GO:0006355 P96900 NC_000962.3 Mycobrowser_v5 CDS 3687685 3689442 . - 0 Rv3302c glpD2 Involved in aerobic respiration and oxidation of glycerol. Reduces an acceptor and generates glycerone phosphate from Sn-glycerol 3-phosphate. Possibly play a role in metabolism of riboflavin, FAD,FMN [catalytic activity: SN-glycerol 3-phosphate + acceptor = glycerone phosphate + reduced acceptor]. Probable glycerol-3-phosphate dehydrogenase GlpD2 Rv3302c, (MTCI418A.04c, MTV016.01c), len: 585 aa. Probable glpd2, glycerol-3-phosphate dehydrogenase, equivalent to P53435|GLPD_MYCLE|ML0713|L308_C1_179 glycerol-3-phosphate dehydrogenase from Mycobacterium leprae (585 aa), FASTA scores: opt: 3489, E(): 2.2e-198, (90.75% identity in 584 aa overlap). Also highly similar to many e.g. Q9L0I3|SCD63.06 from Streptomyces coelicolor (568 aa), FASTA scores: opt: 2203, E(): 1.6e-122, (59.95% identity in 564 aa overlap); Q9RVK8|DR1019 from Deinococcus radiodurans (522 aa), FASTA scores: opt: 949, E(): 1.4e-48, (37.0% identity in 538 aa overlap); BAB53412|MLR7270 from Rhizobium loti (Mesorhizobium loti) (505 aa), FASTA scores: opt: 861, E(): 2.2e-43, (37.3% identity in 488 aa overlap); P18158|GLPD_BACSU from B. subtilis (555 aa), FASTA scores: opt: 768, E(): 7.2e-38, (32.85% identity in 484 aa overlap); etc. Also similar to Mycobacterium tuberculosis protein Q10502|GLPD_MYCTU|MTCY427_31c|Rv2249c glycerol-3-phosphate dehydrogenase (516 aa), FASTA scores: opt: 843, E(): 2.6e-42, (36.5% identity in 515 aa overlap). Contains PS00978 FAD-dependent glycerol-3-phosphate dehydrogenase signature 2. Cofactor: FAD (by similarity). Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family. P9WN79 No intermediary metabolism and respiration P64184 P9WN79 GO:0009331,GO:0006072,GO:0055114,GO:0004368 1.1.5.3 P9WN79 NC_000962.3 Mycobrowser_v5 CDS 3689457 3690938 . - 0 Rv3303c lpdA Involved in energy metabolism. Can catalyze the reduction of electron acceptors such as 2,6-dimethyl-1,4-benzoquinone (DMBQ) and 5-hydroxy-1,4-napthaquinone (5-HNQ) [catalytic activity: NAD(P)H + a quinone + H+ -> a quinol + NAD(P)+]. NAD(P)H quinone reductase LpdA Rv3303c, (MTV016.02c), len: 493 aa. Probable lpdA, quinone reductase, similar to e.g. Q9EWV3|2SCK31.22c putative oxidoreductase from Streptomyces coelicolor (475 aa), FASTA scores: opt: 1420, E(): 2.4e-77, (54.9% identity in 471 aa overlap); Q9A7J2|CC1731 lipoamide dehydrogenase (E3 component,pyruvate dehydrogenase complex) from Caulobacter crescentus (466 aa), FASTA scores: opt: 696, E(): 3.6e-34, (29.6% identity in 463 aa overlap); Q04829|LPD|DLDH_HALVO dihydrolipoamide dehydrogenase from Halobacterium volcanii (Haloferax volcanii) (474 aa), FASTA scores: opt: 675, E(): 6.5e-33, (29.3% identity in 471 aa overlap); P50970|DLDH_ZYMMO|LPD dihydrolipoamide dehydrogenase from Zymomonas mobilis, FASTA scores: opt: 658, E(): 6.6e-32, (30.4% identity in 464 aa overlap); etc. Belongs to the pyridine nucleotide-disulfide oxidoreductases class-I. Cofactor: FAD. P9WHH7 No intermediary metabolism and respiration 1XDI O53355 P9WHH7 GO:0003955,GO:0045454,GO:0005737,GO:0004148,GO:0055114,GO:0050660 1.6.5.2 P9WHH7 NC_000962.3 Mycobrowser_v5 CDS 3691141 3691620 . + 0 Rv3304 Rv3304 Function unknown Conserved protein Rv3304, (MTV016.03), len: 159 aa. Conserved protein, very similar to Q9CCL6|ML0711 hypothetical protein from Mycobacterium leprae (159 aa), FASTA scores: opt: 1041, E(): 6.1e-62, (91.8% identity in 159 aa overlap); and Q49927|L308_F3_97 from M. leprae (174 aa), FASTA scores: opt: 974, E(): 1.8e-57, (91.2% identity in 149 aa overlap). Also highly similar to Q9AD81|SCK13.10c conserved hypothetical protein from Streptomyces coelicolor (145 aa), FASTA scores: opt: 615, E(): 7.8e-34, (60.55% identity in 147 aa overlap); and shows some similarity to other various hypotheticals proteins. ORF continues upstream with possible start at 2198 (equivalent to AAK47746 from Mycobacterium tuberculosis strain CDC1551 (212 aa) but shorter 53 aa). O53356 No conserved hypotheticals O53356 O53356 O53356 NC_000962.3 Mycobrowser_v5 CDS 3692805 3693989 . - 0 Rv3306c amiB1 Involved in cellular metabolism, active on carbon aliphatic amides and/or on many aromatic amides [catalytic activity: a monocarboxylic acid amide + H(2)O = a monocarboxylate + NH(3)]. Probable amidohydrolase AmiB1 (aminohydrolase) Rv3306c, (MTV016.05c), len: 394 aa. Probable amiB1, aminohydrolase, similar to several belonging to peptidase family M40 (and to hypothetical proteins) e.g. P54983|AMHX_BACSU amidohydrolase AMHX from Bacillus subtilis (389 aa), FASTA scores: opt: 286, E(): 9.9e-10, (26.6% identity in 351 aa overlap); P76052|ABGB_ECOLI Aminobenzoyl-glutamate utilizatio from Escherichia coli (481 aa), FASTA scores: opt: 383, E(): 2.1e-15, (30.5% identity in 328 aa overlap); P44765|YDAJ_HAEIN hypothetical protein HI0584 from Haemophilus influenzae (423 aa), FASTA scores: opt: 297, E(): 2.4e-10, (29.6% identity in 274 aa overlap). Note that previously known as amiB. L7N690 No intermediary metabolism and respiration Q7D5Q9 L7N690 GO:0008152,GO:0005515,GO:0016787 3.5.1.- L7N690 NC_000962.3 Mycobrowser_v5 CDS 3694054 3694860 . + 0 Rv3307 deoD Involved in purine nucleoside salvage. Cleavage of guanosine or inosine to respective BASES and sugar-1-phosphate molecules [catalytic activity: purine nucleoside + orthophosphate = purine + alpha-D-ribose 1-phosphate]. Probable purine nucleoside phosphorylase DeoD (inosine phosphorylase) (PNP) Rv3307, (MTV016.06), len: 268 aa. Probable deoD (alternate gene name: punA), purine nucleoside phosphorylase, similar to others especially P46862|PUNA_MYCLE|DEOD_MYCLE|ML0707|L308_F2_56 from M. leprae (268 aa), FASTA scores: opt: 1373, E(): 1.5e-74, (82.05% identity in 262 aa overlap); Q9EWV2|2SCK31.24 from Streptomyces coelicolor (274 aa), FASTA scores: opt: 1026, E(): 6.4e-54, (60.5% identity in 266 aa overlap); P81989|PUNA_CELSP from Cellulomonas sp (282 aa), FASTA scores: opt: 963, E(): 3.6e-50, (58.9% identity in 270 aa overlap); Q9X1T2|TM1596 from Thermotoga maritima (265 aa), FASTA scores: opt: 584, E(): 1.1e-27, (39.55% identity in 263 aa overlap); etc. Belongs to the PNP/MTAP family 2 of phosphorylases. P9WP01 No intermediary metabolism and respiration 1G2O,1I80,1N3I,3IOM P0A538 P9WP01 GO:0004731,GO:0009116 2.4.2.1 P9WP01 NC_000962.3 Mycobrowser_v5 CDS 3696470 3697093 . - 0 Rv3309c upp Involved in pyrimidine salvage pathway [catalytic activity: UMP + pyrophosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate]. Probable uracil phosphoribosyltransferase Upp (UMP pyrophosphorylase) (uprtase) (UMP diphosphorylase) Rv3309c, (MTV016.08c), len: 207 aa. Probable upp, uracil phosphoribosyltransferase, identical to P94928|UPP uracil phosphoribosyltransferase from Mycobacterium bovis (207 aa). Also similar to others e.g. P36399|UPP_STRSL from Streptococcus salivarius (209 aa), FASTA scores: opt: 658, E(): 4.7e-35, (48.3% identity in 207 aa overlap); Q9A194|UPP|SPY0392 from Streptococcus pyogenes (209 aa), FASTA scores: opt:650, E(): 1.5e-34, (47.35% identity in 207 aa overlap); Q9RE01|UPP from Lactobacillus plantarum (209 aa), FASTA scores: opt: 644, E(): 3.7e-34, (46.4% identity in 207 aa overlap); etc. Belongs to the uprtase family. P9WFF3 No intermediary metabolism and respiration P0A658 P9WFF3 GO:0004845,GO:0006223,GO:0009116 2.4.2.9 P9WFF3 NC_000962.3 Mycobrowser_v5 CDS 3698121 3699383 . + 0 Rv3311 Rv3311 Function unknown Conserved protein Rv3311, (MTV016.10), len: 420 aa. Conserved protein, equivalent to Mycobacterium leprae hypothetical proteins Q9CCL8|ML0703 (423 aa), FASTA scores: opt: 2185, E(): 5.5e-120, (77.55% identity in 423 aa overlap); Q49918|L308_F2_61 (167 aa), FASTA scores: opt: 929, E(): 3.5e-47, (84.4% identity in 167 aa overlap) (similarity at C-terminus for this one); and Q49914|L308_F1_17 (166 aa), FASTA scores: opt: 900, E(): 1.7e-45, (79.0% identity in 162 aa overlap) (similarity at N-terminus for this one); Q49923|U0308N (86 aa) FASTA scores: opt: 149, E(): 0.052, (48.35% identity in 60 aa overlap); etc. Note that the Rv3311 corresponding protein in Mycobacterium leprae is similar to products of two adjacent ORFs. Also some similarity to Q9XI61|F9L1.1 hypothetical protein from Arabidopsis thaliana (Mouse-ear cress) (523 aa), FASTA scores: opt: 134, E(): 1.8, (25.1% identity in 203 aa overlap). Equivalent to AAK47753 from Mycobacterium tuberculosis strain CDC1551 (431 aa) but shorter 12 aa. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53362 No conserved hypotheticals O53362 NC_000962.3 Mycobrowser_v5 CDS 3703464 3703865 . - 0 Rv3315c cdd This enzyme scavenge exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis [catalytic activity: cytidine + H(2)O = uridine + NH(3)]. Probable cytidine deaminase Cdd (cytidine aminohydrolase) (cytidine nucleoside deaminase) Rv3315c, (MTV016.15c), len: 133 aa. Probable cdd, cytidine deaminase, equivalent to Q9CBD3|CDD|ML2174 cytidine deaminase from Mycobacterium leprae (134 aa), FASTA scores: opt: 516, E(): 5.8e-28, (56.8% identity in 132 aa overlap). Also highly similar to many e.g. Q9AK37|2SCK8.15 from Streptomyces coelicolor (130 aa), FASTA scores: opt: 523, E(): 1.9e-28, (60.0% identity in 130 aa overlap); Q9KD53|CDD|BH1366 from Bacillus halodurans (132 aa), FASTA scores: opt: 305, E(): 9.2e-14, (41.55% identity in 130 aa overlap); P56389|CDD_MOUSE|CDA|CDD from Mus musculus (Mouse) (146 aa), FASTA scores: opt: 287, E(): 1.6e-12, (40.3% identity in 124 aa overlap); P19079|CDD_BACSU (136 aa), FASTA scores: opt: 270, E(): 2.1e-11, (28.6% identity in 127 aa overlap); etc. Contains PS00903 Cytidine and deoxycytidylate deaminases zinc-binding region signature. Belongs to the cytidine and deoxycytidylate deaminases family. Cofactor: zinc (by similarity). P9WPH3 No intermediary metabolism and respiration 3IJF O53367 P9WPH3 GO:0046087,GO:0008270,GO:0004126 3.5.4.5 P9WPH3 NC_000962.3 Mycobrowser_v5 CDS 3767346 3768140 . - 0 Rv3351c Rv3351c Function unknown Conserved hypothetical protein Rv3351c, (MTV004.08c), len: 264 aa. Hypothetical protein, highly similar to C-terminal region (aa 292-479) of O53608|Rv0063|MTV030.06 oxidoreductase from Mycobacterium tuberculosis (479 aa), FASTA scores: opt: 699, E(): 1.7e-36, (54.75% identity in 190 aa overlap). Shows some similarity to Q9KYD6|SCD72A.20 putative lipoprotein (fragment) from Streptomyces coelicolor (403 aa), FASTA scores: opt: 192, E(): 9.1e-05, (27.9% identity in 154 aa overlap); and P71091|YGAK hypothetical 54.4 KDA protein from Bacillus subtilis (480 aa), FASTA scores: opt: 174, E(): 0.0014, (26.5% identity in 166 aa overlap). Note that the two upstream ORFs Rv3352c and Rv3353c also show similarity to Rv0063 (MTV030_7). Sequence was checked but no errors found. Predicted to be an outer membrane protein (See Song et al., 2008). O50380 No conserved hypotheticals O50380 O50380 GO:0016491,GO:0050660 O50380 NC_000962.3 Mycobrowser_v5 CDS 3704102 3704440 . + 0 Rv3316 sdhC Involved in tricarboxylic acid cycle. Mono-heme cytochrome of the succinate dehydrogenase complex. Probable succinate dehydrogenase (cytochrome B-556 subunit) SdhC (succinic dehydrogenase) (fumarate reductase) (fumarate dehydrogenase) (fumaric hydrogenase) Rv3316, (MTV016.16), len: 112 aa. Probable sdhC, cytochrome B-556 of succinate dehydrogenase SdhC subunit , transmembrane protein, equivalent (but shorter 35 aa) to Q9CCM0|SDHC|ML0699 putative succinate dehydrogenase cytochrome B-556 subunit from Mycobacterium leprae (153 aa), FASTA scores: opt: 692, E(): 1.2e-39, (88.4% identity in 112 aa overlap). Also similar to others e.g. Q9KZ88|SC5G8.26c from Streptomyces coelicolor (126 aa), FASTA scores: opt: 484, E(): 8.3e-26, (65.65% identity in 99 aa overlap); Q9RVR8|DR0954 from Deinococcus radiodurans (118 aa), FASTA scores: opt: 195, E(): 1.7e-06, (36.8% identity in 87 aa overlap); Q9HQ63|DHSD_HALN1|SDHD|SDHC|VNG1310G from Halobacterium sp. strain NRC-1 (130 aa), FASTA scores: opt: 192, E(): 2.9e-06, (37.85% identity in 74 aa overlap); P72109|DHSD_NATPH|SDHD|SDHC from Natronomonas pharaonis (Natronobacterium pharaonis) (130 aa), FASTA scores: opt: 183, E(): 1.1e-05, (35.15% identity in 74 aa overlap); etc. Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, cytochrome B-556, and an hydrophobic anchor protein. Belongs to the cytochrome B560 family. O53368 No intermediary metabolism and respiration O53368 O53368 GO:0000104,GO:0055114 1.3.99.1 O53368 NC_000962.3 Mycobrowser_v5 CDS 3713394 3714332 . - 0 Rv3328c sigJ The sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released. Probable alternative RNA polymerase sigma factor (fragment) SigJ Rv3328c, (MTV016.28c), len: 312 aa. Probable sigJ, alternative RNA polymerase sigma factor (see citations below), highly similar to many e.g. Q9K3H7|2SCG18.10c from Streptomyces coelicolor (295 aa), FASTA scores: opt: 642, E(): 7.3e-31, (42.8% identity in 292 aa overlap); Q9A3D8|CC3266 from Caulobacter crescentus (291 aa), FASTA scores: opt: 607, E(): 8.4e-29, (39.8% identity in 294 aa overlap); Q9RD74|SCF43.14c from Streptomyces coelicolor (324 aa), FASTA scores: opt: 555, E(): 1.1e-25, (41.1% identity in 297 aa overlap); etc. Similar also to U00022_20 from Mycobacterium leprae; and MTCI28_22 and MSU87307_1. Also similar to O50445|SIGI|Rv1189|MTV005.25|MTCI364.01 putative RNA polymerase sigma factor from Mycobacterium tuberculosis (290 aa), FASTA scores: opt: 426, E(): 4.2e-18, (32.65% identity in 294 aa overlap). Equivalent to AAK47774 from Mycobacterium tuberculosis strain CDC1551 (282 aa) but longer 30 aa. Contains probable helix-turn-helix motif at aa 129-150 (Score 1126, +3.02 SD). Belongs to the sigma-70 factor family, ECF subfamily. L0TCG5 No information pathways O53378 L0TCG5 GO:0016987,GO:0003700,GO:0006352,GO:0006355 L0TCG5 NC_000962.3 Mycobrowser_v5 CDS 3724615 3725844 . - 0 Rv3339c icd1 Involved in the KREBS cycle [catalytic activity: isocitrate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH]. Probable isocitrate dehydrogenase [NADP] Icd1 (oxalosuccinate decarboxylase) (IDH) (NADP+-specific ICDH) (IDP) Rv3339c, (MTV016.39c), len: 409 aa. Probable icd1, isocitrate dehydrogenase NADP-dependent, highly similar to many e.g. Q9A5C8|CC2522 from Caulobacter crescentus (403 aa), FASTA scores: opt: 1972, E(): 4.6e-115, (72.45% identity in 403 aa overlap); AAF73472|ICD from Rhizobium meliloti (404 aa), FASTA scores: opt: 1968, E(): 8.1e-115, (73.2% identity in 403 aa overlap); P50215|IDH_SPHYA from Sphingomonas yanoikuyae (406 aa), FASTA scores: opt: 1964, E(): 1.4e-114, (71.45% identity in 403 aa overlap); etc. Contains PS00470 Isocitrate and isopropylmalate dehydrogenases signature. Belongs to the isocitrate and isopropylmalate dehydrogenases family. Note that in H37Rv, Rv0066c is named icd2 and Rv3339c is icd1 while in CDC1551 and Erdman strains, Rv0066c is icd1 and Rv3339c is icd2. P9WKL1 No intermediary metabolism and respiration P65097 P9WKL1 GO:0006097,GO:0004450,GO:0006102,GO:0000287,GO:0055114,GO:0006099,GO:0051287 1.1.1.42 P9WKL1 NC_000962.3 Mycobrowser_v5 CDS 3726127 3727476 . + 0 Rv3340 metC Transforms O-acetylhomoserine into L-methionine [catalytic activity: O-acetyl-L-homoserine + methanethiol = L-methionine + acetate]. Probable O-acetylhomoserine sulfhydrylase MetC (homocysteine synthase) (O-acetylhomoserine (thiol)-lyase) (OAH sulfhydrylase) (O-acetyl-L-homoserine sulfhydrylase) Rv3340, (MTV016.40), len: 449 aa. Probable metC, O-acetyl-L-homoserine sulfhydrylase, highly similar to many e.g. Q9K9P2|BH2603 O-acetylhomoserine sulfhydrylase from Bacillus halodurans (430 aa), FASTA scores: opt: 1716, E(): 3.3e-97, (60.45% identity in 425 aa overlap); Q9HUE4|METY|PA5025 homocysteine synthase from Pseudomonas aeruginosa (425 aa), FASTA scores: opt: 1517, E(): 4.4e-85, (56.95% identity in 425 aa overlap); Q9WZY4|TM0882 O-acetylhomoserine sulfhydrylase from Thermotoga maritima (430 aa), FASTA scores: opt: 1488, E(): 2.6e-83, (55.75% identity in 418 aa overlap); BAB54344|MLR8465 O-acetylhomoserine sulfhydrylase from Rhizobium loti (Mesorhizobium loti) (426 aa), FASTA scores: opt: 1445, E(): 1.1e-80, (53.2% identity in 419 aa overlap); P50125|CYSD_EMENI O-acetylhomoserine (thiol)-lyase from Emericella nidulans (Aspergillus nidulans) (437 aa), FASTA scores: opt: 1442, E(): 1.7e-80, (53.7% identity in 430 aa overlap); etc. Contains PS00868 Cys/Met metabolism enzymes pyridoxal-phosphate attachment site. Cofactor: pyridoxal phosphate. Belongs to the trans-sulfuration enzymes family. O53390 No intermediary metabolism and respiration O53390 O53390 GO:0006520,GO:0016829,GO:0030170,GO:0003961 2.5.1.49 O53390 NC_000962.3 Mycobrowser_v5 tRNA 3348659 3348730 . - 0 MTB000040 glnU - tRNA-Gln (CTG) glnU, tRNA-Gln; anticodon ctg, length = 72 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 3769804 3770649 . - 0 Rv3356c folD Necessary for the biosynthesis of purines, thymydylate, methionine, histidine, pantothenate, and formyl tRNA-met [catalytic activity: 5,10-methylenetetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH] [catalytic activity: 5,10-methenyltetrahydrofolate + H(2)O = 10-formyltetrahydrofolate]. Probable bifunctional protein FolD: methylenetetrahydrofolate dehydrogenase + methenyltetrahydrofolate cyclohydrolase Rv3356c, (MTV004.13c), len: 281 aa. Probable folD, bifunctional enzyme include methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase , equivalent to O32879|fold|ML0674 methylenetetrahydrofolate dehydrogenase (putative methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase) from Mycobacterium leprae (282 aa), FASTA scores: opt: 1624, E(): 1.2e-93, (86.45% identity in 281 aa overlap). Also similar to many others e.g. Q9K3J6|fold from Streptomyces coelicolor (284 aa), FASTA scores: opt: 1223, E(): 9.5e-69, (66.65% identity in 279 aa overlap); Q9K966|fold from Bacillus halodurans (279 aa), FASTA scores: opt: 886, E(): 7.7e-48, (47.15% identity in 280 aa overlap); P54382|FOLD_BACSU from Bacillus subtilis (283 aa), FASTA scores: opt: 820, E(): 9.7e-44, (45.7% identity in 280 aa overlap); P51696|FOLD_PHOPO from Photobacterium phosphoreum (285 aa), FASTA scores: opt: 778, E(): 4e-41, (44.9% identity in 283 aa overlap); P24186|FOLD_ECOLI|ads|B0529 from Escherichia coli (287 aa), FASTA scores: opt: 741, E(): 0,44.4, (44.4% identity in 277 aa overlap); etc. Also highly similar to MLCB1779_9 from Mycobacterium leprae cosmid B1779 (282 aa) (86.5% identity in 281 aa overlap). Similar to other dehydrogenase/cyclohydrolase enzymes or domains. P9WG81 No intermediary metabolism and respiration 2C2X,2C2Y O50385 P9WG81 GO:0009396,GO:0000105,GO:0004477,GO:0009086,GO:0004488,GO:0006730,GO:0055114,GO:0006164 3.5.4.9,1.5.1.5 P9WG81 NC_000962.3 Mycobrowser_v5 CDS 3771344 3772534 . + 0 Rv3359 Rv3359 Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv3359, (MTV004.16), len: 396 aa. Possible oxidoreductase, similar to N-terminal part of various proteins (hypothetical unknowns or oxidoreductases) e.g. Q9ZB94 hypothetical 69.3 KDA protein from Rhodococcus erythropolis (649 aa), FASTA scores: opt: 509, E(): 3e-24, (30.0% identity in 380 aa overlap); O29991|AF0248 NADH-dependent flavin oxidoreductase from Archaeoglobus fulgidus (378 aa), FASTA scores: opt: 478, E(): 1.6e-22, (32.45% identity in 379 aa overlap); Q9HUH9|PA4986 probable oxidoreductase from Pseudomonas aeruginosa (648 aa), FASTA scores: opt: 412, E(): 3.3e-18, (30.45% identity in 384 aa overlap); Q9KCT8|BH1481 NADH oxidase from Bacillus halodurans (338 aa), FASTA scores: opt: 404, E(): 6.1e-18, (30.2% identity in 275 aa overlap); etc. Some weak similarity to Mycobacterium leprae MLCB1779_10. O50388 No intermediary metabolism and respiration O50388 O50388 GO:0055114,GO:0016491,GO:0010181 1.-.-.- O50388 NC_000962.3 Mycobrowser_v5 CDS 3793257 3794867 . - 0 Rv3379c dxs2 Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP). Possibly involved in deoxyxylulose-5-phosphate pathway (DXP) of isoprenoid biosynthesis (at the first step), and biosynthetic pathway to thiamine andpyridoxol (at the first step). Probable 1-deoxy-D-xylulose 5-phosphate synthase Dxs2 (1-deoxyxylulose-5-phosphate synthase) (DXP synthase) (DXPS) Rv3379c, (MTV004.37c), len: 536 aa. Probable dxs2, 1-deoxy-D-xylulose 5-phosphate synthase, similar to many e.g. Q9F1V2|DXS from Kitasatospora griseola (Streptomyces griseolosporeus) (649 aa), FASTA scores: opt: 1274, E(): 5.4e-71, (50.9% identity in 570 aa overlap); Q9X7W3|DXS_STRCO|SC6A5.17 from Streptomyces coelicolor (656 aa), FASTA scores: opt: 1248, E(): 2.2e-69, (50.55% identity in 568 aa overlap); Q9RBN6|DXS_STRC1 from Streptomyces sp. strain CL190 (631 aa), FASTA scores: opt: 1237, E(): 1e-68, (49.1% identity in 570 aa overlap); Q50000|DXS_MYCLE|TKTB|ML1038 from Mycobacterium leprae (643 aa), FASTA scores: opt: 1215, E(): 2.4e-67, (46.75% identity in 571 aa overlap); Q9R6S7|DXS_SYNLE from Synechococcus leopoliensis (636 aa), FASTA scores: opt: 849, E(): 8.9e-45, (38.55% identity in 550 aa overlap); etc. Also similar to O07184|DXS_MYCTU|Rv2682c|MT2756|MTCY05A6.03c from Mycobacterium tuberculosis (638 aa), FASTA scores: opt: 1226, E(): 4.9e-68, (48.9% identity in 558 aa overlap). Belongs to the transketolase family, DXS subfamily. Cofactor: thiamine pyrophosphate (by similarity). Note that the N-terminus of this putative protein appears to have been interrupted by the adjacent IS6110 element. O50408 No intermediary metabolism and respiration O50408 O50408 GO:0016740,GO:0008152 2.2.-.- O50408 NC_000962.3 Mycobrowser_v5 CDS 3777737 3778201 . + 0 Rv3366 spoU Causes methylation Probable tRNA/rRNA methylase SpoU (tRNA/rRNA methyltransferase) Rv3366, (MTV004.23), len: 154 aa. Probable spoU, tRNA/rRNA methylase, equivalent to Q9CCU7|ML0419 putative tRNA/rRNA methyltransferase from Mycobacterium leprae (158 aa), FASTA scores: opt: 861, E(): 1.2e-50, (83.75% identity in 154 aa overlap); and O07698|MLCL383.24c rRNA methylase from Mycobacterium leprae (169 aa), FASTA scores: opt: 861, E(): 1.3e-50, (83.75% identity in 154 aa overlap). Also highly similar to many members of the spoU family of rRNA methylases e.g. Q9K199|NMB0268 RNA methyltransferase (TRMH family) from Neisseria meningitidis (serogroup B) (154 aa), FASTA scores: opt: 534, E(): 7.6e-29, (50.0% identity in 154 aa overlap); and Q9JSM8|NMA2218 from Neisseria meningitidis (serogroup A) (154 aa), FASTA scores: opt: 526, E(): 2.6e-28, (49.35% identity in 154 aa overlap); Q9HU57|PA5127 from Pseudomonas aeruginosa (153 aa), FASTA scores: opt: 531, E(): 1.2e-28, (52.95% identity in 151 aa overlap); P33899|YIBK_ECOLI|B3606 from Escherichia coli strain K12 (157 aa), FASTA scores: opt: 511, E(): 2.6e-27, (49.35% identity in 154 aa overlap); etc. Belongs to the RNA methyltransferase TrmH family. O50394 No information pathways O50394 O50394 GO:0008173,GO:0006396,GO:0003723 2.1.1.- O50394 NC_000962.3 Mycobrowser_v5 CDS 3781501 3784740 . - 0 Rv3370c dnaE2 DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The alpha chain is the DNA polymerase. [catalytic activity: N deoxynucleoside triphosphate = N pyrophosphate + DNA(N)]. Probable DNA polymerase III (alpha chain) DnaE2 (DNA nucleotidyltransferase) Rv3370c, (MTV004.28c), len: 1079 aa. Probable dnaE2, DNA polymerase III, alpha chain (see citations below), similar to many e.g. BAB51086|MLR4428 from Rhizobium loti (Mesorhizobium loti) (1118 aa), FASTA scores: opt: 1103, E(): 8.9e-59, (37.65% identity in 1075 aa overlap); Q9S291|SCI11.28c from Streptomyces coelicolor (1185 aa), FASTA scores: opt: 937, E(): 1e-48, (33.4% identity in 1090 aa overlap); O67125|DP3A_AQUAE|DNAE|AQ_1008 from Aquifex aeolicus (1161 aa), FASTA scores: opt: 895, E(): 3.4e-46, (29.9% identity in 1071 aa overlap); O51526|DP3A_BORBU from Borrelia burgdorferi (Lyme disease spirochete) (1147 aa), FASTA scores: opt: 835, E(): 1.4e-42, (30.05% identity in 888 aa overlap); etc. Equivalent to AAK47817 from Mycobacterium tuberculosis strain CDC1551 (1098 aa) but shorter 19 aa. Also similar to Mycobacterium tuberculosis DP3A_MYCTU|MTCY48.18c|dnaE1 (29.6% identity in 1110 aa overlap). Belongs to DNA polymerase type-C family, DNAE subfamily. P9WNT5 No information pathways O50399 P9WNT5 GO:0003677,GO:0006281,GO:0006260,GO:0003887,GO:0005737,GO:0008408 2.7.7.7 P9WNT5 NC_000962.3 Mycobrowser_v5 CDS 3786314 3787489 . + 0 Rv3372 otsB2 Involved in osmoregulatory trehalose biosynthesis. Mycobacteria can produce trehalose from glucose 6-phosphate and UDP-glucose (the OtsA-OtsB pathway) from glycogen-like alpha(1-->4)-linked glucose polymers (the TreY-TreZ pathway) and from maltose (the TreS pathway) [catalytic activity: trehalose 6-phosphate + H(2)O = trehalose + orthophosphate]. Trehalose 6-phosphate phosphatase OtsB2 (trehalose-phosphatase) (TPP) Rv3372, (MTV004.30), len: 391 aa. otsB2, trehalose-6-phosphate phosphatase, equivalent to Q49734|OTSB2|OTSP|B1620_F1_1|MLCL383.17c putative trehalose-phosphatase from Mycobacterium leprae (429 aa), FASTA scores: opt: 1675, E(): 2.4e-91, (67.05% identity in 425 aa overlap). Also weakly similar to several trehalose phosphatases e.g. Q9C8B3|F10O5.8 from Arabidopsis thaliana (Mouse-ear cress) (366 aa), FASTA scores: opt: 432, E(): 3.1e-18, (36.65% identity in 281 aa overlap); O27788|MTH1760 from Methanobacterium thermoautotrophicum (264 aa), FASTA scores: opt: 347, E(): 2.5e-13, (30.75% identity in 221 aa overlap); Q9FWQ2 from Oryza sativa (Rice) (382 aa), FASTA scores: opt: 338, E(): 1.1e-12, (32.5% identity in 320 aa overlap); etc. Also similar to part of Mycobacterium tuberculosis Q10850|YK06_MYCTU|Rv2006|MT2062|MTCY39.11c (1327 aa), FASTA scores: opt: 1192, E(): 1.6e-62, (56.65% identity in 339 aa overlap). P9WFZ5 No virulence, detoxification, adaptation O50401 P9WFZ5 GO:0004805,GO:0005992 3.1.3.12 P9WFZ5 NC_000962.3 Mycobrowser_v5 CDS 3787726 3788367 . + 0 Rv3373 echA18 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Probable enoyl-CoA hydratase EchA18 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv3373, (MTV004.31), len: 213 aa. Probable echA18, enoyl-CoA hydratase, similar to others e.g. P97087|CRT from Clostridium thermosaccharolyticum (Thermoanaerobacterium thermosaccharolyticum) (259 aa), FASTA scores: opt: 423, E(): 3.4e-20, (37.95% identity in 174 aa overlap); Q9X7Q4|SC5F2A.31c from Streptomyces coelicolor (257 aa), FASTA scores: opt: 399, E(): 1.2e-18, (45.05% identity in 171 aa overlap); BAB52005|MLL5584 from Rhizobium loti (Mesorhizobium loti) (257 aa), FASTA scores: opt: 385, E(): 9.6e-18, (41.95% identity in 174 aa overlap); etc. Also some similarity to 3-hydroxybutyryl-CoA dehydratases e.g. P52046|CRT_CLOAB from Clostridium acetobutylicum (261 aa), FASTA scores: opt: 414, E(): 1.3e-19, (38.3% identity in 175 aa overlap). And similar to other hydratases from Mycobacterium tuberculosis e.g. O53418|ECH8_MYCTU|Rv1070c|MT1100|MTV017.23c probable enoyl-CoA hydratase (257 aa), FASTA scores: opt: 365, E(): 1.9e-16, (39.1% identity in 174 aa overlap). Belongs to the enoyl-CoA hydratase/isomerase family. Note that this homology extends across the stop codon and directly into the next ORF MTV004.29, suggesting a possible readthrough of the TGA stop codon. O50402 No lipid metabolism O50402 O50402 GO:0008152,GO:0004300 4.2.1.17 O50402 NC_000962.3 Mycobrowser_v5 CDS 3790156 3790809 . + 0 Rv3376 Rv3376 Function unknown Conserved hypothetical protein Rv3376, (MTV004.34), len: 217 aa. Hypothetical protein, similar to various bacterial proteins (notably hydrolases) e.g. Q9RUP0|DR1344 hydrolase from Deinococcus radiodurans (222 aa), FASTA scores: opt: 348, E(): 1.8e-15, (36.75% identity in 215 aa overlap); Q9RXA1|DR0414 hydrolase (CBBY/CBBZ/GPH/YIEH family) from Deinococcus radiodurans (155 aa), FASTA scores: opt: 233, E(): 3.5e-08, (36.4% identity in 151 aa overlap); Q9X0Q9|TM1177 conserved hypothetical protein from Thermotoga maritima (225 aa), FASTA scores: opt: 231, E(): 6.6e-08, (27.6% identity in 221 aa overlap); Q9ABI3|CC0244 hydrolase, haloacid dehalogenase-like from Caulobacter crescentus (213 aa), FASTA scores: opt: 213, E(): 9.1e-07, (28.95% identity in 221 aa overlap); BAB38231|ECS4808 putative phosphatase from Escherichia coli strain O157:H7 (206 aa), FASTA scores: opt: 210, E(): 1.4e-06, (26.95% identity in 193 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WMS5 No conserved hypotheticals O50405 P9WMS5 GO:0008967,GO:0008152 P9WMS5 NC_000962.3 Mycobrowser_v5 CDS 3790848 3792353 . - 0 Rv3377c Rv3377c Produces halimadienyl diphosphate (tuberculosinyl diphosphate) from geranylgeranyl diphosphate (GGPP) [catalytic activity: geranylgeranyl diphosphate = halima-5(6),13-DIEN-15-YL diphosphate] Halimadienyl diphosphate synthase Rv3377c, (MTV004.35c), len: 501 aa. Halimadienyl diphosphate synthase; similarity with various proteins, notably cyclases involved in steroid biosynthesis in plants and bacteria e.g. BAB52679|MLR6369 from Rhizobium loti (Mesorhizobium loti) (516 aa), FASTA scores: opt: 533, E(): 5.6e-27, (30.45% identity in 522 aa overlap); Q9ZTN8 copalyl diphosphate synthase 1 from Cucurbita maxima (Pumpkin) (Winter squash) (823 aa), FASTA scores: opt: 484, E(): 1.2e-23, (28.35% identity in 388 aa overlap); Q38710|AC22 abietadiene cyclase from Abies grandis (868 aa), FASTA scores: opt: 382, E(): 5.2e-17, (25.55% identity in 462 aa overlap); Q41771|AN1 kaurene synthase a from Zea mays (Maize) (823 aa), FASTA scores: opt: 377, E(): 1.1e-16, (29.75% identity in 390 aa overlap); Q9AJE4 diterpene cyclase-1 from Kitasatospora griseola (Streptomyces griseolosporeus) (499 aa), FASTA scores: opt: 336, E(): 3.2e-14, (27.5% identity in 513 aa overlap); Q9SAU6 E-alpha-bisabolene synthase (fragment) from Abies grandis (782 aa), FASTA scores: opt: 317, E(): 7.8e-13, (25.25% identity in 479 aa overlap); etc. Note that this and the upstream ORF MTV004.36c have a significantly lower GC bias than the rest of the genome. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Cofactor: Mg2+. O50406 No intermediary metabolism and respiration O50406 5.5.1.16 O50406 NC_000962.3 Mycobrowser_v5 CDS 3801653 3803848 . + 0 Rv3388 PE_PGRS52 Function unknown PE-PGRS family protein PE_PGRS52 Rv3388, (MTV004.46), len: 731 aa. PE_PGRS52, Member of the M. tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), similar to many PE-family proteins from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. O53553|YZ08_MYCTU|RV3508|MTV023.15 (1901 aa), FASTA scores: opt: 2380, E(): 3.6e-87, (53.8% identity in 773 aa overlap); and MTV023_21, MTV023_18, MTV023_14, MTV039_16, MTCY441_4. Predicted to be an outer membrane protein (See Song et al., 2008). Q6MWX5 No PE/PPE Q6MWX5 Q6MWX5 Q6MWX5 NC_000962.3 Mycobrowser_v5 CDS 3807574 3808437 . - 0 Rv3392c cmaA1 Has cyclopropane function. Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge. Mycolic acids, which represent the major constituent of mycobacterial cell wall complex, act as substrates [catalytic activity: S-adenosyl-L-methionine + phospholipidolefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid]. Cyclopropane-fatty-acyl-phospholipid synthase 1 CmaA1 (cyclopropane fatty acid synthase) (CFA synthase) (cyclopropane mycolic acid synthase 1) Rv3392c, (MTV004.50), len: 287 aa. CmaA1, cyclopropane mycolic acid synthase 1, characterized in 1995 as CFA1_MYCTU|Q11195|CMAA1|CMA1 cyclopropane-fatty-acyl-phospholipid synthase 1 (see citations below). Highly similar to Mycobacterium tuberculosis proteins MTCY20H10.23c (58.7% identity in 286 aa overlap); MTCY20H10.24c (68.6% identity); MTCY20H10.25c (73.5% identity); MTCY20H10.26c (57.0% identity); and MTCY20G9.30c (55.7% identity). Also highly similar to Q9CBK3|MMAA4|ML1903 methyl mycolic acid synthases from Mycobacterium leprae (298 aa), FASTA scores: opt: 1098, E(): 1e-63, (57.0% identity in 286 aa overlap). Equivalent to AAK44898|MT0672 from Mycobacterium tuberculosis strain CDC1551 (317 aa) but shorter 30 aa and with some differences in residues between the proteins. P9WPB7 No lipid metabolism 1KP9,1KPG,1KPH P0C5C2 P9WPB7 GO:0005737,GO:0008610,GO:0008825 2.1.1.79 P9WPB7 NC_000962.3 Mycobrowser_v5 CDS 3808461 3809387 . + 0 Rv3393 iunH Involved in purine salvage. Catalyzes the hydrolysis of all of the commonly occurring purine and pyrimidine nucleosides into ribose and the associated base, and could have a preference for inosine and uridine as substrates [catalytic activity: a N-D-ribosylpurine + H(2)O = a purine + D- ribose]. Probable nucleoside hydrolase IunH (purine nucleosidase) Rv3393, (MTV004.51), len: 308 aa. Probable iunH, nucleoside hydrolase, similar to others e.g. Q9RXB2|DR0403 from Deinococcus radiodurans (314 aa), FASTA scores: opt: 497, E(): 6e-24, (34.3% identity in 312 aa overlap); Q27546|IUNH_CRIFA from Crithidia fasciculata (314 aa), FASTA scores: opt: 475, E(): 1.4e-22, (31.45% identity in 318 aa overlap); Q9CK67|IUNH from Pasteurella multocida (310 aa), FASTA scores: opt: 464, E(): 6.9e-22, (30.9% identity in 314 aa overlap); Q9A549|CC2615 from Caulobacter crescentus (323 aa), FASTA scores: opt: 464, E(): 7.2e-22, (37.85% identity in 280 aa overlap); etc. Note that also similar to BAB34113|ECS0690 (alias AAG54985|YBEK) putative tRNA synthetase from Escherichia coli strain O157:H7 (311 aa), FASTA scores: opt: 483, E(): 4.5e-23, (33.0% identity in 315 aa overlap). The active site histidine is conserved. O50418 No intermediary metabolism and respiration O50418 O50418 GO:0008477,GO:0008152 3.2.2.1 O50418 NC_000962.3 Mycobrowser_v5 CDS 4192850 4193245 . - 0 Rv3742c Rv3742c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv3742c, (MTV025.090c), len: 131 aa. Possible oxidoreductase, probably combines with product of downstream ORF MTV025.090c to form a functional monooxygenase, highly similar to N-terminal end of various oxidoreductases e.g. Q9A588|CC2569 monooxygenase (flavin-binding family) from Caulobacter crescentus (498 aa), FASTA scores: opt: 170, E(): 0.00048, (47.55% identity in 103 aa overlap); Q9APW3 aromatic-ring hyroxylase from Pseudomonas aeruginosa (508 aa) FASTA scores: opt: 160, E(): 0.0022, (50.55% identity in 87 aa overlap); Q9RZT0|DRB0033 arylesterase/monoxygenase from Deinococcus radiodurans (833 aa), FASTA scores: opt: 153, E(): 0.0097, (45.45% identity in 88 aa overlap); etc. Also similar to C-terminal end of Mycobacterium tuberculosis proteins (generally monooxygenases) e.g. P96223|Rv3854c|MTCY01A6.14 hypothetical 55.3 KDA protein (489 aa), FASTA scores: opt: 140, E(): 0.044, (37.1% identity in 132 aa overlap); O53300|Rv3083|MTV013.04 monoxygenase (495 aa) FASTA scores: opt: 133, E(): 0.13, (43.05% identity in 79 aa overlap); O53762|Rv0565c|MTV039.03c putative monoxygenase (486 aa), FASTA scores: opt: 110, E(): 4.1, (42.85% identity in 77 aa overlap); etc. Note similarity to MTCY01A6.14 and MTV013.04 continue in downstream ORF (MTV025.089c) after a gap of ~100 aa. O69709 No intermediary metabolism and respiration O69709 GO:0016491,GO:0055114 1.-.-.- O69709 NC_000962.3 Mycobrowser_v5 CDS 3812501 3814078 . - 0 Rv3396c guaA Involved in GMP biosynthesis [catalytic activity: ATP + xanthosine 5'-phosphate + L-glutamine + H(2)O = AMP + pyrophosphate + GMP + L-glutamate]. Probable GMP synthase [glutamine-hydrolyzing] GuaA (glutamine amidotransferase) (GMP synthetase) Rv3396c, (MTCY78.32), len: 525 aa. Probable guaA, gmp synthase (see citation below), equivalent to P46810|GUAA_MYCLE|ML0395|B1620_C2_205 GMP synthase [glutamine-hydrolyzing] from Mycobacterium leprae (529 aa), FASTA scores: opt: 2992, E(): 8.5e-168, (86.85% identity in 525 aa overlap). Also highly similar to others e.g. O52831|GUAA_CORAM from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (524 aa), FASTA scores: opt: 2636, E(): 5.9e-147, (76.2% identity in 521 aa overlap); Q9L0H2|GUAA_STRCO from Streptomyces coelicolor (526 aa), FASTA scores: opt: 2451, E(): 4.1e-136, (71.55% identity in 513 aa overlap); Q9KF78|GUAA_BACHD from Bacillus Halodurans (513 aa), FASTA scores: opt: 1819, E(): 4.1e-99, (52.55% identity in 510 aa overlap); etc. Contains PS00442 Glutamine amidotransferases class-I active site. Belongs to the type-1 glutamine amidotransferase family in the N-terminal section. And belongs to the GMP synthase family in the C-terminal section. P9WMS7 No intermediary metabolism and respiration P0A5A1 P9WMS7 GO:0006177,GO:0003922,GO:0006541,GO:0005524 6.3.5.2 P9WMS7 NC_000962.3 Mycobrowser_v5 CDS 3814090 3814998 . - 0 Rv3397c phyA Involved in carotenoid biosynthesis and in astaxanthin biosynthetic pathway. Catalyses the reaction from prephytoene diphosphate to phytoene [catalytic activity 1: 2 geranylgeranyl diphosphate = pyrophosphate + prephytoene diphosphate] [catalytic activity 2: prephytoene diphosphate = pyrophosphate + phytoene]. Probable phytoene synthase PhyA Rv3397c, (MTCY78.31), len: 302 aa. Probable phyA (alternate gene name: crtB), phytoene synthase, similar to many others e.g. Q9X7V5|SC6A5.09 from Streptomyces coelicolor (312 aa), FASTA scores: opt: 791, E(): 2.8e-43, (48.25% identity in 286 aa overlap); Q9RW07|DR0862 from Deinococcus radiodurans (325 aa), FASTA scores: opt: 482, E(): 1.5e-23, (35.25% identity in 292 aa overlap); Q9JRU9|NMB1168|NMB1130 from Neisseria meningitidis (serogroup B) (290 aa), FASTA scores: opt: 446, E(): 2.8e-21, (34.25% identity in 260 aa overlap); P37272|PSY_CAPAN from Capsicum annuum (Bell pepper) (419 aa), FASTA scores: opt: 431, E(): 3.4e-20, (33.0% identity in 288 aa overlap); etc. Also similar to Q9JUF5|NMA1339 putative poly-isoprenyl transferase from Neisseria meningitidis (serogroup A) (290 aa), FASTA scores: opt: 450, E(): 1.6e-21, (34.6% identity in 260 aa overlap). And similar to crtB|O05424 phytoene synthase from Mycobacterium marinum (319 aa), blastp scores: 113, E= 6e-24, Identities = 89/283 (31%) (see citation below). Contains PS01045 Squalene and phytoene synthases signature 2. Belongs to the phytoene/squalene synthetase family. P9WHP3 No intermediary metabolism and respiration P65860 P9WHP3 GO:0016767,GO:0016117 2.5.1.32 P9WHP3 NC_000962.3 Mycobrowser_v5 CDS 3817239 3818027 . + 0 Rv3400 Rv3400 Function unknown; probably involved in cellular metabolism. Probable hydrolase Rv3400, (MTCY78.28c), len: 262 aa. Probable hydrolase, strongly equivalent to Q49741|YY00_MYCLE|ML0393|B1620_F3_119 hypothetical 28.6 KDA protein from Mycobacterium leprae (261 aa), FASTA scores: opt: 1293, E(): 2.2e-71, (74.45% identity in 262 aa overlap). Similar to several various proteins (notably hydrolases) e.g. Q9L2I7|SCF42.32 putative hydrolase from Streptomyces coelicolor (246 aa), FASTA scores: opt: 888, E(): 7.7e-47, (56.35% identity in 245 aa overlap); Q9EX06|2SCG38.13 putative hydrolase from Streptomyces coelicolor (238 aa), FASTA scores: opt: 195, E(): 8.1e-05, (29.5% identity in 234 aa overlap); Q9I5X4|PA0562 probable hydrolase from Pseudomonas aeruginosa (224 aa), FASTA scores: opt: 190, E(): 0.00015, (27.8% identity in 248 aa overlap); O06995|PGMB_BACSU|YVDM putative beta-phosphoglucomutase from Bacillus subtilis (226 aa), FASTA scores: opt: 190, E(): 0.00016, (33.9% identity in 245 aa overlap); etc. Also similar to Mycobacterium tuberculosis hypothetical protein Q10850|YK06_MYCTU|Rv2006|MT2062|MTCY39.11c (1327 aa), FASTA scores: opt: 413, E(): 2e-17, (34.9% identity in 238 aa overlap). Interestingly, note that Rv3400 and Rv3401 are similar to beginning and end of Q10850|YK06_MYCTU|Rv2006|MT2062|MTCY39.11c with approx. 270 aa missing from the middle. P9WKZ7 No intermediary metabolism and respiration P65069 P9WKZ7 GO:0008152,GO:0003824 P9WKZ7 NC_000962.3 Mycobrowser_v5 CDS 3820653 3821891 . - 0 Rv3402c Rv3402c Function unknown; thought to be involved in cell process. Conserved hypothetical protein Rv3402c, (MTCY78.26), len: 412 aa. Conserved hypothetical protein, probably involved in cell process, similar to various proteins generally involved in extracellular compounds (lipopolysaccharide O-antigen) biosynthesis e.g. O68392|RFBE perosamine synthetase from Brucella melitensis (367 aa), FASTA scores: opt: 420, E(): 1.2e-19, (26.15% identity in 375 aa overlap); Q9L6C1 3,4-dehydratase-like protein from Streptomyces antibioticus (393 aa), FASTA scores: opt: 419, E(): 1.5e-19, (30.65% identity in 385 aa overlap); Q9RR26|OLENI dehydratase from Streptomyces antibioticus (393 aa), FASTA scores: opt: 416, E(): 2.3e-19, (30.65% identity in 385 aa overlap); O33942 eryciv protein from Saccharopolyspora erythraea (Streptomyces erythraeus) (401 aa), FASTA scores: opt: 410, E(): 5.6e-19, (31.75% identity in 362 aa overlap); Q9UZI4|ASPB-LIKE1|PAB0774 aspartate aminotransferase (ASPB-LIKE1) from Pyrococcus abyssi (366 aa), FASTA scores: opt: 402, E(): 1.7e-18, (27.05% identity in 377 aa overlap); O88001|WLBC putative amino-sugar biosynthesis protein from Bordetella bronchiseptica (Alcaligenes bronchisepticus) (366 aa), FASTA scores: opt: 394, E(): 5.6e-18, (26.8% identity in 347 aa overlap); Q45378|BPLC DNA for lipopolysaccharide biosynthesis from Bordetella pertussis (366 aa), FASTA scores: opt: 393, E(): 6.5e-18, (26.8% identity in 347 aa overlap); etc. P9WGJ7 No cell wall and cell processes Q50723 P9WGJ7 GO:0030170,GO:0003824 P9WGJ7 NC_000962.3 Mycobrowser_v5 CDS 4030493 4031407 . + 0 Rv3589 mutY Involved in base excision repair. Removes adenine mispaired with 8-OXOG. May repair A.G and A.C mismatches by adenine excision. Probable adenine glycosylase MutY Rv3589, (MTV024.07), len: 304 aa. Probable mutY, adenine glycosylase (see citation below), equivalent to Q9CBJ0|MUTY|ML1920 probable DNA glycosylase from Mycobacterium leprae (297 aa), FASTA scores: opt: 1592, E(): 2.6e-94, (74.9% identity in 303 aa overlap). Also similar to many DNA glycosylases (generally adenine glycosylases) e.g. Q9S6T7|SCE94.06 from Streptomyces coelicolor (308 aa), FASTA scores: opt: 965, E(): 2.6e-54, (50.5% identity in 297 aa overlap); Q9S6G1|MUTY from Streptomyces antibioticus (307 aa), FASTA scores: opt: 901, E(): 3.1e-50, (48.5% identity in 303 aa overlap); Q9HPQ6|MUTY|VNG1520G from Halobacterium sp. strain NRC-1 (312 aa), FASTA scores: opt: 566, E(): 7.2e-29, (39.85% identity in 296 aa overlap); BAB53965|MLL7523 from Rhizobium loti (Mesorhizobium loti) (396 aa), FASTA scores: opt: 511, E(): 2.8e-25, (39.65% identity in 237 aa overlap); Q05869|MUTY_SALTY|MUTB from Salmonella typhimurium (350 aa), FASTA scores: opt: 421, E(): 3.8e-20, (35.2% identity in 227 aa overlap); etc. Could belong to the nth/MUTY family. P9WQ09 No information pathways O53574 P9WQ09 GO:0003677,GO:0006284,GO:0004519,GO:0016798,GO:0051539 3.2.2.- P9WQ09 NC_000962.3 Mycobrowser_v5 CDS 3828783 3829910 . - 0 Rv3410c guaB3 Catalyses the first reaction unique to GMP biosynthesis [catalytic activity: inosine 5'-phosphate + NAD(+) + H(2)O = xanthosine 5'-phosphate + NADH]. Probable inosine-5'-monophosphate dehydrogenase GuaB3 (imp dehydrogenase) (inosinic acid dehydrogenase) (inosinate dehydrogenase) (imp oxidoreductase) (inosine-5'-monophosphate oxidoreductase) (IMPDH) (IMPD) Rv3410c, (MTCY78.18), len: 375 aa. Probable guaB3, inosine-5'-monophosphate (imp) dehydrogenase, equivalent to Q49721|YY10_MYCLE|ML0388|B1620_C2_193 hypothetical 38.9 KDA protein from Mycobacterium leprae (375 aa), FASTA scores: opt: 2182, E(): 9.5e-122, (90.6% identity in 373 aa overlap). Highly similar to Q9RHY9 GUAB ORF genes for imp dehydrogenase, hypothetical protein from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (376 aa), FASTA scores: opt: 1490, E(): 7.6e-81, (61.0% identity in 382 aa overlap); Q9L0I6|SCD63.03 putative inosine-5'-monophosphate dehydrogenase from Streptomyces coelicolor (374 aa), FASTA scores: opt: 1275, E(): 3.8e-68, (52.95% identity in 372 aa overlap); P73853|GUAB|SLR1722 imp dehydrogenase subunit from Synechocystis sp. strain PCC 6803 (387 aa), FASTA scores: opt: 882, E(): 6.7e-45, (41.3% identity in 373 aa overlap); and similar to other inosine-5'-monophosphate dehydrogenases e.g. P44334|IMDH_HAEIN|GUAB|HI0221 from Haemophilus influenzae (488 aa), FASTA scores: opt: 267, E(): 1.8e-08, (34.25% identity in 216 aa overlap); etc. Also highly similar to the C-terminus of Q50753|GUAA/B homology to Mycobacterium leprae GUAA (fragment) from Mycobacterium tuberculosis (130 aa), FASTA scores: opt: 506, E(): 4.6e-23, (85.05% identity in 87 aa overlap). Similar to other eukaryotic and prokaryotic IMPDH and to GMP reductase. P9WKI5 No intermediary metabolism and respiration P65170 P9WKI5 GO:0016491,GO:0055114 1.-.-.- P9WKI5 NC_000962.3 Mycobrowser_v5 CDS 3834892 3835200 . + 0 Rv3416 whiB3 Involved in transcriptional mechanism (growth phase-dependent). Transcriptional regulatory protein WhiB-like WhiB3. Contains [4FE-4S] cluster. Rv3416, (MTCY78.13c), len: 102 aa. WhiB3 (alternate gene name: whmB), WhiB-like regulatory protein (see citations below), similar to WhiB paralogue of Streptomyces coelicolor, wblE gene product (85 aa). Equivalent to Q49871|WHIB3|WHIB|ML0382|B229_F1_2|B1620_F3_137 probable transcription factor WHIB3 from Mycobacterium leprae (102 aa), FASTA scores: opt: 657, E(): 7.9e-39, (86.25% identity in 102 aa overlap). Also highly similar to Q9Z6E9|WHIB3 from Mycobacterium smegmatis (96 aa), FASTA scores: opt: 604, E(): 3.5e-35, (80.4% identity in 102 aa overlap); and O88103|WHID|SC6G4.45c|WBLB from Streptomyces coelicolor (112 aa), FASTA scores: opt: 437, E(): 1.4e-23, (62.5% identity in 96 aa overlap). Also similar to O05847|WHIB1|Rv3219|MTCY07D11.07c from Mycobacterium tuberculosis (84 aa), FASTA scores: opt: 215, E(): 2.5e-08, (44.45% identity in 81 aa overlap). Note that primer extension analysis revealed three transcriptional start sites and that expression from the three potential promoters is growth phase-dependent (see Mulder et al., 1999). Moreover, the transcription of this CDS seems to be activated in macrophages (see Ramakrishnan et al., 2000). [4Fe-4S] cluster is degraded by oxygen and reacts with no (See Singh et al., 2007). P9WF41 No regulatory proteins Q50710 P9WF41 P9WF41 NC_000962.3 Mycobrowser_v5 CDS 3835272 3836891 . - 0 Rv3417c groEL1 Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions. 60 kDa chaperonin 1 GroEL1 (protein CPN60-1) (GroEL protein 1) Rv3417c, (MTCY78.12), len: 539 aa. GroEL1 (alternate genbe name: cpn60_1), 60 kDa chaperonin 1 (protein cpn60 1) (see citations below), equivalent to P37578|CH61_MYCLE|B1620_C3_228|GROL1|GROEL1|GROEL-1|GROE1|ML0381|B229_ 60 KDA chaperonin 1 from Mycobacterium leprae (537 aa), FASTA scores: opt: 2846, E(): 1.5e-154, (82.95% identity in 539 aa overlap). Also highly similar to others e.g. Q00767|CH61_STRAL|GROL1|GROEL1 from Streptomyces albus G (539 aa), FASTA scores: opt: 2130, E(): 8.1e-114, (61.9% identity in 541 aa overlap); P40171|CH61_STRCO|GROL1|GROEL1|SC6G4.40 from Streptomyces coelicolor (540 aa), FASTA scores: opt: 2119, E(): 3.4e-113, (61.8% identity in 542 aa overlap); etc. Also similar to P06806|CH62_MYCTU|Q48931|Rv0440|MTV037.04|GROL2|GROEL2|GROEL-2|HSP65 (62.2% identity in 527 aa overlap). Contains PS00017 ATP/GTP-binding site motif A, PS00296 Chaperonins cpn60 signature. Belongs to the chaperonin (HSP60) family. P9WPE9 No virulence, detoxification, adaptation 3M6C P0A518 P9WPE9 GO:0005737,GO:0042026,GO:0051082,GO:0005524 P9WPE9 NC_000962.3 Mycobrowser_v5 CDS 3837555 3838589 . - 0 Rv3419c gcp Hydrolysis of O-sialoglycoproteins; cleaves 31-ARG-|-asp-32 bond in glycophorin A. Does not cleave unglycosylated proteins, desialylated glycoproteins or glycoproteins that are only N-glycosylated. Could be a metalloprotease. Probable O-sialoglycoprotein endopeptidase Gcp (glycoprotease) Rv3419c, (MTCY78.10), len: 344 aa. Probable gcp, glycoprotease, equivalent to P37969|GCP_MYCLE|GCP|ML0379|U229E|U1620c|B229_C3_246|B1620_C3_226 probable glycoprotease from Mycobacterium leprae (351 aa), FASTA scores: opt: 1898, E(): 2.4e-101, (86.1% identity in 345 aa overlap). Highly similar to others e.g. O86793|GCP_STRCO|GCP|SC6G4.30 from Streptomyces coelicolor (374 aa), FASTA scores: opt: 1282, E(): 4.1e-66, (60.45% identity in 344 aa overlap); Q9WXZ2|TM0145 from Thermotoga maritima (327 aa), FASTA scores: opt: 867, E(): 1.9e-42, (45.4% identity in 337 aa overlap); P05852|GCP_ECOLI|B3064 from Escherichia coli strain K12 (337 aa), FASTA scores: opt: 838, E(): 9e-41, (46.55% identity in 346 aa overlap); etc. Shows some similarity to Q50707|YY21_MYCTU|Rv3421c|MTCY78.08 (33.9% identity in 127 aa overlap). Contains PS01016 Glycoprotease family signature. Belongs to peptidase family M22; also known as the glycoprotease family. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P9WHT7 No intermediary metabolism and respiration P65801 P9WHT7 GO:0006508,GO:0008270,GO:0004222 3.4.24.57 P9WHT7 NC_000962.3 Mycobrowser_v5 CDS 3850372 3851754 . - 0 Rv3432c gadB Catalyzes the production of GABA [catalytic activity: L-glutamate = 4-aminobutanoate + CO(2)]. Probable glutamate decarboxylase GadB Rv3432c, (MTCY77.04c), len: 460 aa. Probable gadB, glutamate decarboxylase, similar to many e.g. P73043|gad|SLL1641 from Synechocystis sp. strain PCC 6803 (467 aa), FASTA scores: opt: 1684, E(): 6.2e-99, (55.35% identity in 457 aa overlap); Q9X8J5|SCE9.23 from Streptomyces coelicolor (475 aa), FASTA scores: opt: 1650, E(): 8.9e-97, (57.4% identity in 446 aa overlap); Q9AQU4|gad from Oryza sativa (Rice) (501 aa), FASTA scores: opt: 1498, E(): 3.7e-87, (51.6% identity in 432 aa overlap); Q07346|DCE_PETHY from Petunia hybrida (Petunia) (500 aa), FASTA scores: opt: 1485, E(): 2.5e-86, (51.15% identity in 437 aa overlap); etc. Belongs to group II decarboxylases (DDC, gad, HDC and TYRDC). I6YG46 No intermediary metabolism and respiration O06249 I6YG46 GO:0006536,GO:0030170,GO:0004351 4.1.1.15 I6YG46 NC_000962.3 Mycobrowser_v5 CDS 3855015 3856889 . - 0 Rv3436c glmS Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source [catalytic activity: L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate]. Probable glucosamine--fructose-6-phosphate aminotransferase [isomerizing] GlmS (hexosephosphate aminotransferase) (D-fructose-6-phosphate amidotransferase) (GFAT) (L-glutamine-D-fructose-6-phosphate amidotransferase) (glucosamine-6-phosphate synthase) Rv3436c, (MTCY77.08c), len: 624 aa. Probable glmS, glucosamine--fructose-6-phosphate aminotransferase, equivalent to P40831|GLMS_MYCLE|ML0371|B229_C3_238 glucosamine--fructose-6-phosphate aminotransferase [isomerizing] from Mycobacterium leprae (623 aa), FASTA scores: opt: 3584, E(): 4.7e-214, (89.3% identity in 627 aa overlap). Also highly similar to others e.g. O68956|GLMS_MYCSM from Mycobacterium smegmatis (627 aa), FASTA scores: opt: 3517, E(): 6.5e-210, (87.25% identity in 627 aa overlap); O86781|GLMS_STRCO|SC6G4.18 from Streptomyces coelicolor (614 aa), FASTA scores: opt: 2364, E(): 1.3e-138, (64.95% identity in 625 aa overlap); Q9K1P9|NMB0031 from Neisseria meningitidis (serogroup B) and Q9JWN9|GLMS|NMA0276 from Neisseria meningitidis (serogroup A) (612 aa), FASTA scores: opt: 1445, E(): 8.4e-82, (43.55% identity in 627 aa overlap); etc. Belongs to the type-2 gatase domain in the N-terminal section. Belongs to the sis family, GLMS subfamily, in the C-terminal section. P9WN49 No intermediary metabolism and respiration P0A588 P9WN49 GO:0005737,GO:0006541,GO:0004360,GO:0005529,GO:0016051 2.6.1.16 P9WN49 NC_000962.3 Mycobrowser_v5 CDS 3860024 3861370 . - 0 Rv3441c mrsA Function unknown; involved in cellular metabolism. Probable phospho-sugar mutase / MrsA protein homolog Rv3441c, (MTCY77.13c), len: 448 aa. Probable mrsA, phosphoglucomutase or phosphomannomutase, equivalent to Q49869|URED|B229_C3_234 MRSA protein homolog from Mycobacterium leprae (463 aa), FASTA scores: opt: 2449, E(): 6.3e-135, (87.65% identity in 445 aa overlap); and highly similar (but longer 178 aa) to Q49862|UREC|B229_C2_192 putative urease operon UREC protein from Mycobacterium leprae (288 aa), FASTA scores: opt: 1442, E(): 1.3e-76, (86.5% identity in 267 aa overlap). Highly similar to phospho-sugar mutases e.g. Q53876|SC6G4.14 putative phospho-sugar mutase (similar to phosphomannomutases) from Streptomyces coelicolor (452 aa), FASTA scores: opt: 1710, E(): 5e-92, (60.45% identity in 450 aa overlap); Q9KG46|BH0267 phosphoglucosamine mutase from Bacillus halodurans (447 aa), FASTA scores: opt: 1351, E(): 3.5e-71, (48.4% identity in 444 aa overlap); BAB58323|GLMM phosphoglucosamine-mutase from Staphylococcus aureus subsp. aureus Mu50 (451 aa) and Q99QR5|GLMM(FEMD)|SA1965 phosphoglucosamine-mutase from Staphylococcus aureus subsp. aureus N315. (451 aa), FASTA scores: opt: 1315, E(): 4.3e-69, (48.45% identity in 446 aa overlap); P95685|FEMD|GLMM phosphoglucosamine-mutase (451 aa), FASTA scores: opt: 1310, E(): 8.5e-69, (48.2% identity in 446 aa overlap); P95575|MRSA_PSESY MRSA protein homolog from Pseudomonas syringae (pv. syringae) (447 aa), FASTA scores: opt: 1143, E(): 4.2e-59, (42.75% identity in 447 aa overlap); etc. Contains PS00710 Phosphoglucomutase and phosphomannomutase phosphoserine signature. Belongs to the phosphohexose mutases family. P9WN41 No intermediary metabolism and respiration O06258 P9WN41 GO:0000287,GO:0008966,GO:0005975 5.4.2.10 P9WN41 NC_000962.3 Mycobrowser_v5 CDS 3861947 3862390 . - 0 Rv3443c rplM Involved in translation mechanism. This protein is one of the early assembly proteins of the 50S ribosomal subunit. 50S ribosomal protein L13 RplM Rv3443c, (MTCY77.15c), len: 147 aa. rplM, 50S ribosomal protein L13, equivalent to P38014|RL13_MYCLE|RPLM|ML0364|B229_C3_232 from Mycobacterium leprae (147 aa), FASTA scores: opt: 917, E(): 7.5e-53, (91.15% identity in 147 aa overlap). Also highly similar to others e.g. Q53874|RL13_STRCO|RPLM|SC6G4.12 from Streptomyces coelicolor (147 aa), FASTA scores: opt: 668, E(): 1.1e-36, (65.5% identity in 145 aa overlap); Q9X1G5|RL13_THEMA|RPLM|TM1454 from Thermotoga maritima (149 aa), FASTA scores: opt: 536, E(): 4.4e-28, (53.65% identity in 136 aa overlap); O67722|RL13_AQUAE|RPLM|AQ_1877 from Aquifex aeolicus (144 aa), FASTA scores: opt: 529, E(): 1.2e-27, (53.2% identity in 141 aa overlap); etc. Belongs to the L13P family of ribosomal proteins. P9WHE1 No information pathways P66065 P9WHE1 GO:0003735,GO:0006412,GO:0005840 P9WHE1 NC_000962.3 Mycobrowser_v5 CDS 3877464 3878507 . - 0 Rv3457c rpoA DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The amino-terminal portion is involved in the assembly of core RNAP, whereas the C-terminal is involved in interaction with transcriptional regulators [catalytic activity: N nucleoside triphosphate = N pyrophosphate + RNA(N)]. Probable DNA-directed RNA polymerase (alpha chain) RpoA (transcriptase alpha chain) (RNA polymerase alpha subunit) (DNA-directed RNA nucleotidyltransferase) Rv3457c, (MTCY13E12.10c), len: 347 aa. Probable rpoA, alpha chain of RNA polymerase, equivalent to Q9X798|RPOA_MYCLE|ML1957|MLCB1222.27c DNA-directed RNA polymerase alpha from Mycobacterium leprae (347 aa), FASTA scores: opt: 2139, E(): 1.3e-123, (95.65% identity in 347 aa overlap). Also highly similar to others e.g. P72404|RPOA_STRCO|C6G4.07 from Streptomyces coelicolor (340 aa), FASTA scores: opt: 1672, E(): 4.7e-95, (75.55% identity in 348 aa overlap); Q9X4V6|RPOA_STRGT from Streptomyces granaticolor (340 aa), FASTA scores: opt: 1671, E(): 5.4e-95, (75.55% identity in 348 aa overlap); P20429|RPOA_BACSU from Bacillus subtilis (314 aa), FASTA scores: opt: 939, E(): 3e-50, (48.9% identity in 311 aa overlap); etc. Contains (PS00017) ATP/GTP-binding site motif A (P-loop). Belongs to the RNA polymerase alpha chain family. P9WGZ1 No information pathways P66701 P9WGZ1 GO:0003899,GO:0046983,GO:0006351,GO:0003677 2.7.7.6 P9WGZ1 NC_000962.3 Mycobrowser_v5 CDS 3879696 3880070 . - 0 Rv3460c rpsM Involved in the binding of fMet-tRNA and, hence, in the initiation of translation. 30S ribosomal protein S13 RpsM Rv3460c, (MTCY13E12.13c), len: 124 aa. rpsM, 30S ribosomal protein S13, equivalent to Q9X7A1|RS13_MYCLE|RPSM|ML1960|MLCB1222.30c 30S ribosomal protein S13 from Mycobacterium leprae (124 aa), FASTA scores: opt: 762, E(): 1.5e-43, (92.75% identity in 124 aa overlap); and P45813|RS13_MYCBO|RPSM from Mycobacterium bovis (123 aa), FASTA scores: opt: 727, E(): 3e-41, (98.25% identity in 114 aa overlap). Also highly similar to others e.g. O86773|RS13_STRCO|SC6G4.05 from Streptomyces coelicolor (126 aa), FASTA scores: opt: 631, E(): 6.2e-35, (73.75% identity in 122 aa overlap); Q9RA65|RPS13 from Thermus aquaticus (subsp. thermophilus) (126 aa), FASTA scores: opt: 552, E(): 9.8e-30, (62.6% identity in 123 aa overlap); P20282|RS13_BACSU|RPSM from Bacillus subtilis (120 aa), FASTA scores: opt: 533, E(): 1.7e-28, (64245% identity in 121 aa overlap); etc. Contains ribosomal protein S13 signature (PS00646). Belongs to the S13P family of ribosomal proteins. P9WH61 No information pathways O06327 P9WH61 GO:0005840,GO:0003735,GO:0000049,GO:0006412,GO:0019843 P9WH61 NC_000962.3 Mycobrowser_v5 CDS 3880432 3880653 . - 0 Rv3462c infA No specific function has so far been attributed to this initiation factor; however, it seems to stimulate more or less all the activities of the other two initiation factors, if-2 and if-3. Probable translation initiation factor if-1 InfA Rv3462c, (MTCY13E12.15c), len: 73 aa. Probable infA, initiation factor if-1, equivalent to P45957|ML1962|INFA translation initiation factor if-1 from Mycobacterium bovis (72 aa) and Mycobacterium leprae (72 aa), FASTA scores: opt: 472, E(): 6.6e-28, (100.0% identity in 72 aa overlap). Also highly similar to others e.g. O54209|IF1_STRCO|INFA|SC6G4.03 from Streptomyces coelicolor (73 aa), FASTA scores: opt: 424, E(): 2e-24, (84.95% identity in 73 aa overlap); O50630|IF1_BACHD|INFA|BH0158 from Bacillus halodurans (71 aa), FASTA scores: opt: 388, E(): 8.1e-22, (77.8% identity in 72 aa overlap); Q9XD14|IF1_LEPIN|INFA from Leptospira interrogans (71 aa), FASTA scores: opt: 376, E(): 6e-21, (80.0% identity in 70 aa overlap); etc. Contains 1 'S1 motif' domain. Belongs to the if-1 family. P9WKK3 No information pathways 3I4O P0A5H5 P9WKK3 GO:0005737,GO:0003743,GO:0006413,GO:0003723 P9WKK3 NC_000962.3 Mycobrowser_v5 CDS 3881837 3882832 . + 0 Rv3464 rmlB Involved in dTDP-L-rhamnose biosynthesis [catalytic activity: dTDP-glucose = dTDP-4-dehydro-6-deoxy-D-glucose + H(2)O]. dTDP-glucose 4,6-dehydratase RmlB Rv3464, (MTCY13E12.17), len: 331 aa. RmlB (alternate gene name: rfbB), dTDP-glucose-4,6-dehydratase (see citations below), nearly identical to Q50556|RMLB rhamnose biosynthesis protein from Mycobacterium tuberculosis (329 aa) (previously rfbB, now known as rmlB). Equivalent to Q9CBH7|RMLB|ML1964 dTDP-glucose 4,6-dehydratase (alias Q9X7A3|RMLB putative dTDP-(glucose or rhamnose)-4,6-dehydratase (331 aa)) from Mycobacterium leprae (333 aa), FASTA scores: opt: 1925, E(): 1.9e-112, (84.0% identity in 331 aa overlap). Also highly similar to others e.g. Q9UZH2|RFBB|PAB0785 from Pyrococcus abyssi (333 aa), FASTA scores: opt: 1115, E(): 4.2e-62, (51.55% identity in 322 aa overlap); O27817|MTH1789 from Methanobacterium thermoautotrophicum (336 aa), FASTA scores: opt: 1104, E(): 2.1e-61, (51.65% identity in 331 aa overlap); BAB60064|TVG0950610 from Thermoplasma volcanium (318 aa), FASTA scores: opt: 1102, E(): 2.6e-61, (49.65% identity in 310 aa overlap); etc. Also related to P72050|MTCY13D12.18|RV3784 hypothetical 36.3 KDA protein (similar to galactowaldenases from eukaryotic and prokaryotic origin) from Mycobacterium tuberculosis (326 aa), FASTA scores: E(): 1.4e-26, (33.8% identity in 320 aa overlap). P9WN65 No intermediary metabolism and respiration O06329 P9WN65 GO:0008460,GO:0009225,GO:0050662 4.2.1.46 P9WN65 NC_000962.3 Mycobrowser_v5 CDS 3886073 3887083 . - 0 Rv3469c mhpE Involved in aromatic hydrocarbons degradation [catalytic activity: 4-hydroxy-2-oxovalerate = pyruvate + acetaldehyde]. Probable 4-hydroxy-2-oxovalerate aldolase MhpE (HOA) Rv3469c, (MTCY13E12.22c), len: 336 aa. Probable mhpE, 4-hydroxy-2-oxovalerate aldolase, similar to others (principally from Pseudomonas species) e.g. Q99PZ1|SCP1.301|SCP1.53c from Streptomyces coelicolor (338 aa), FASTA scores: opt: 615, E(): 7.9e-31, (37.65% identity in 332 aa overlap); Q9X9Q0|NIKB NIKB protein (see Bruntner et al., 1999) from Streptomyces tendae (357 aa), FASTA scores: opt: 571, E(): 4.4e-28, (34.5% identity in 339 aa overlap); P51014|BPHF_PSES1 from Pseudomonas sp. strain KKS102 (352 aa), FASTA scores: opt: 549, E(): 9.9e-27, (31.2% identity in 314 aa overlap); Q51983|CMTG_PSEPU from Pseudomonas putida (350 aa), FASTA scores: opt: 543, E(): 2.3e-26, (30.7% identity in 319 aa overlap); P51020|MHPE_ECOLI|MHPF|B0352 from Escherichia coli strain K12 (337 aa), FASTA scores: opt: 517, E(): 9.1e-25, (31.75% identity in 312 aa overlap); etc. Also similar to P71867|MTCY03C7.22|Rv3534c hypothetical 36.4 KDA protein from Mycobacterium tuberculosis (346 aa), FASTA scores: E(): 7.5e-24, (31.9% identity in 310 aa overlap). O06334 No intermediary metabolism and respiration O06334 O06334 GO:0008152,GO:0016829 4.1.3.- O06334 NC_000962.3 Mycobrowser_v5 CDS 3889948 3890733 . - 0 Rv3473c bpoA Supposedly involved in detoxification reactions. Possible peroxidase BpoA (non-haem peroxidase) Rv3473c, (MTCY13E12.26c), len: 261 aa. Possible bpoA, peroxidase (non-haem peroxidase), similar to various enzymes or hypothetical unknown proteins e.g. O85849 hypothetical 26.2 KDA protein from Sphingomonas aromaticivorans (247 aa), FASTA scores: opt: 684, E(): 4.9e-34, (43.8% identity in 242 aa overlap); AAK45412|MT1155 hydrolase, alpha/beta hydrolase fold family from Mycobacterium tuberculosis strain CDC1551 (311 aa), FASTA scores: opt: 675, E(): 2e-33, (39.45% identity in 256 aa overlap); Q9K3V0|SCD10.27 putative hydrolase from Streptomyces coelicolor (352 aa), FASTA scores: opt: 248, E(): 9.7e-08, (26.05% identity in 261 aa overlap); P29715|BPA2_STRAU|BPOA2 non-haem bromoperoxidase (bromide peroxidase) (277 aa), FASTA scores: opt: 237, E(): 3.6e-07, (29.45% identity in 265 aa overlap); O31168|PRXC_STRAU|CPO|CPOT non-heme chloroperoxidase (278 aa), FASTA scores: opt: 236, E(): 4.2e-07, (29.45% identity in 265 aa overlap); AAK62388|T5L19.180 lipase-like protein from Arabidopsis thaliana (Mouse-ear cress) (350 aa), FASTA scores: opt: 236, E(): 5.1e-07, (26.65% identity in 274 aa overlap); etc. Also similar to O06575|BPOB|Rv1123c|MTCY22G8.12c hypothetical 32.5 KDA protein from Mycobacterium tuberculosis (302 aa), FASTA scores: opt: 675, E(): 2e-33, (39.45% identity in 256 aa overlap). Equivalent to AAK47936 from Mycobacterium tuberculosis strain CDC1551 (294 aa) but shorter 33 aa. May have been inactivated or truncated by neighbouring IS6110. O06338 No virulence, detoxification, adaptation O06338 O06338 GO:0055114,GO:0004601 1.11.1.- O06338 NC_000962.3 Mycobrowser_v5 CDS 3892371 3893720 . - 0 Rv3476c kgtP Involved in active transport of dicarboxylic acid across the membrane. Responsible for the translocation of the substrate across the membrane. Probable dicarboxylic acid transport integral membrane protein KgtP (dicarboxylate transporter) Rv3476c, (MTCY13E12.29c), len: 449 aa. Probable kgtP, dicarboxylate-transport integral membrane protein, possibly member of major facilitator superfamily (MFS), highly similar to others e.g. Q9HT43|PA5530 from Pseudomonas aeruginosa (435 aa), FASTA scores: opt: 1209, E(): 2.3e-68, (47.05% identity in 425 aa overlap); Q9I6Q9|PCAT|PA0229 from Pseudomonas aeruginosa (432 aa), FASTA scores: opt: 1131, E(): 1.8e-63, (40.4% identity in 438 aa overlap); Q9WWZ2 from Pseudomonas putida (429 aa), FASTA scores: opt: 1090, E(): 6.5e-61, (41.2% identity in 425 aa overlap); P17448|KGTP_ECOLI|WITA|B2587 from Escherichia coli strain K12 (432 aa), FASTA scores: opt: 1083, E(): 1.8e-60, (40.05% identity in 422 aa overlap); etc. Also similar to O05301|MTCI364.12|Rv1200 hypothetical 44.6 KDA protein from Mycobacterium tuberculosis (425 aa), FASTA scores: E(): 5.2e-25, (28.5% identity in 382 aa overlap). Contains sugar transport protein signatures 1 and 2 (PS00216, PS00217). Belongs to the sugar transporter family. I6XHB8 No cell wall and cell processes O06339 I6XHB8 GO:0016021,GO:0055085,GO:0005215,GO:0008643 NC_000962.3 Mycobrowser_v5 CDS 3906174 3907007 . - 0 Rv3487c lipF Function unknown; lipolytic enzyme involved in cellular metabolism. Probable esterase/lipase LipF Rv3487c, (MTCY13E12.41c), len: 277 aa. Probable lipF, esterase/lipase (see citation below), highly similar, but shorter 50 aa, to O53424|LIPU|Rv1076|MTV017.29 putative esterase/lipase from Mycobacterium tuberculosis (297 aa), FASTA scores: opt: 1229, E(): 3.3e-71, (76.4% identity in 246 aa overlap); and similar to other putative lipases from Mycobacterium tuberculosis e.g. P71759|LIPK|RV2385|MTCY253.36c (306 aa), FASTA scores: opt: 468, E(): 1.2e-22, (36.2% identity in 254 aa overlap). Equivalent, but shorter 79 aa, to Q9ZBM4|MLCB1450.08|ML0314 putative hydrolase (putative esterase) from Mycobacterium leprae (335 aa), FASTA scores: opt: 1225, E(): 6.6e-71, (73.6% identity in 250 aa overlap). Also similar to esterases and lipases of around 300 aa e.g. Q44087|est esterase precursor from Acinetobacter lwoffii (303 aa), FASTA scores: opt: 428, E(): 4.3e-20, (31.85% identity in 251 aa overlap); P18773|EST_ACICA esterase from Acinetobacter calcoaceticus (303 aa), FASTA scores: opt: 420, E(): 1.4e-19, (31.5% identity in 251 aa overlap); Q9KIU1 esterase from uncultured bacterium Plasmid pAH116 (308 aa), FASTA scores: opt: 405, E(): 1.3e-18, (35.1% identity in 242 aa overlap); Q9X8J4|SCE9.22 putative esterase from Streptomyces coelicolor (266 aa), FASTA scores: opt: 390, E(): 1e-17, (35.85% identity in 237 aa overlap); etc. Equivalent to AAK47950 from Mycobacterium tuberculosis strain CDC1551 (327 aa) but shorter 50 aa. O06350 No intermediary metabolism and respiration O06350 O06350 GO:0008152,GO:0016787 3.-.-.- O06350 NC_000962.3 Mycobrowser_v5 CDS 3902150 3902812 . - 0 Rv3483c Rv3483c Function unknown Possible exported protein Rv3483c, (MTCY13E12.36c), len: 220 aa. Possible exported protein, similar to Q9CC94|ML1099 putative lipoprotein from Mycobacterium leprae (202 aa), FASTA scores: opt: 276, E(): 1.4e-08, (33.1% identity in 148 aa overlap). Also showing similarity with Mycobacterium tuberculosis proteins Q11065|LPRE_MYCTU|LPRE|Rv1252c|MT1291|MTCY50.30. putative lipoprotein precursor (202 aa), FASTA scores: opt: 276, E(): 1.4e-08, (29.5% identity in 200 aa overlap); O53445|Rv1097c|MTV017.50c hypothetical 29.9 KDA protein (293 aa), FASTA scores: opt: 161, E(): 0.047, (25.4% identity in 118 aa overlap); P71882|LPPP_MYCTU|Rv2330c|MT2392|MTCY3G12.04 putative lipoprotein precursor (175 aa), FASTA scores: opt: 146, E(): 0.21, (28.25% identity in 184 aa overlap); and O06170|Rv2507|MTCY07A7.13 hypothetical 28.5 KDA protein (273 aa), FASTA scores: opt: 148, E(): 0.23, (25.15% identity in 191 aa overlap). Contains possible N-terminal signal sequence I6X7F2 No cell wall and cell processes I6X7F2 NC_000962.3 Mycobrowser_v5 CDS 3914531 3915886 . - 0 Rv3496c mce4D Unknown, but thought to be involved in host cell invasion. Predicted to be involved in lipid catabolism. Mce-family protein Mce4D Rv3496c, (MTV023.03c), len: 451 aa. Mce4D; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07416|Rv0172|MTCI28.12|mce1D (530 aa); O07786|Rv0592|MTCY19H5.30c|mce2D (508 aa); and O53970|Rv1969|MTV051.07|mce3D (423 aa). Also similar to others e.g. Q9CD11|MCE1D|ML2592 putative secreted protein from Mycobacterium leprae (531 aa), FASTA scores: opt: 837, E(): 2.6e-34, (34.55% identity in 446 aa overlap); Q9F359|SC8A2.04c putative secreted protein from Streptomyces coelicolor (337 aa), FASTA scores: opt: 606, E(): 4.9e-23, (32.35% identity in 300 aa overlap); etc. Hydrophobic region at N-terminus. Predicted to be an outer membrane protein (See Song et al., 2008). I6XHD6 No virulence, detoxification, adaptation O53541 I6XHD6 I6XHD6 NC_000962.3 Mycobrowser_v5 CDS 3957521 3958432 . + 0 Rv3521 Rv3521 Function unknown Conserved hypothetical protein Rv3521, (MTV023.28), len: 303 aa. Conserved hypothetical protein, similar to (although longer than) other conserved hypothetical proteins e.g. O29296|AF0966 from Archaeoglobus fulgidus (176 aa), FASTA scores: opt: 286, E(): 5.4e-11, (31.15% identity in 170 aa overlap); O30036|AF0203 from Archaeoglobus fulgidus (149 aa) FASTA scores: opt: 259, E(): 2.3e-09, (33.8% identity in 142 aa overlap); O29297|AF0965 from Archaeoglobus fulgidus (154 aa), FASTA scores: opt: 241, E(): 3.2e-08, (31.4% identity in 137 aa overlap); Q9Y995|APE2390 from Aeropyrum pernix (157 aa), FASTA scores: opt: 204, E(): 6.8e-06, (27.45% identity in 153 aa overlap); BAB60424|TVG1322512 from Thermoplasma volcanium (164 aa), FASTA scores: opt: 183, E(): 0.00015, (29.75% identity in 148 aa overlap); etc. Equivalent to AAK47982 from Mycobacterium tuberculosis strain CDC1551 (334 aa) but shorter 31 aa. O53566 No conserved hypotheticals O53566 O53566 O53566 NC_000962.3 Mycobrowser_v5 CDS 3920097 3920861 . - 0 Rv3501c yrbE4A Unknown. Predicted to be involved in lipid catabolism. Conserved integral membrane protein YrbE4A. Possible ABC transporter. Rv3501c, (MTV023.08c), len: 254 aa. YrbE4A, conserved integral membrane protein, part of mce4 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07412|Rv0167|MTCI28.07|yrbE1A (265 aa); O07791|Rv0587|MTCY19H5.35|yrbE2A (265 aa); and O53965|Rv1964|MTV051.02|yrbE3A (265 aa). Also highly similar to conserved hypothetical integral membrane proteins of the P45030|YRBE_HAEIN (261 aa) type, e.g. Q9CD16|YRBE1A|ML2587 from Mycobacterium leprae (267 aa), FASTA scores: opt: 1059, E(): 1e-57, (64.75% identity in 247 aa overlap); P45030|YRBE_HAEIN|HI1086 from Haemophilus influenzae (261 aa), FASTA scores: opt: 313, E(): 3e-14, (25.7% identity in 241 aa overlap); etc. O53546 No virulence, detoxification, adaptation O53546 O53546 O53546 NC_000962.3 Mycobrowser_v5 CDS 3921087 3922040 . - 0 Rv3502c Rv3502c Function unknown; supposed involvement in cellular metabolism. Predicted to be involved in lipid catabolism. Probable short-chain type dehydrogenase/reductase. Possible 17-beta-hydroxysteroid dehydrogenase. Rv3502c, (MTV023.09c), len: 317 aa (start uncertain). Probable short-chain dehydrogenase/reductase , similar to Mycobacterium tuberculosis proteins P71853|Rv3548c|MTCY03C7.08 hypothetical 31.1 KDA protein (304 aa), FASTA scores: opt: 739, E(): 6.2e-35, (45.15% identity in 310 aa overlap); and Q11020|YD50_MYCTU|FABG2|Rv1350|MT1393|MTCY02B10.14 putative oxidoreductase (247 aa), FASTA scores: opt: 475, E(): 5.1e-20, (40.15% identity in 254 aa overlap). Also similar to various dehydrogenases e.g. Q9I4V1|PA1023 probable short-chain dehydrogenase from Pseudomonas aeruginosa (305 aa), FASTA scores: opt: 535, E(): 2.3e-23, (37.1% identity in 302 aa overlap); Q9UVH9|FOX2 FOX2 protein (SDR family) (1015 aa), FASTA scores: opt: 487, E(): 3.2e-20, (38.4% identity in 276 aa overlap); P22414|FOX2_CANTR peroxisomal hydratase-dehydrogenase, D-3-hydroxyacyl CoA dehydrogenase (SDR family) from Candida tropicalis (Yeast) (906 aa) FASTA scores: opt: 481, E(): 6.4e-20, (38.0% identity in 250 aa overlap); P50171|DHB8_MOUSE|HSD17B8|HKE6|H2-KE6 estradiol 17 beta-dehydrogenase 8 from Mus musculus (Mouse) (260 aa) FASTA scores: opt: 459, E(): 4.3e-19, (39.75% identity in 259 aa overlap); CAC41362|BKR1 3-oxyacyl-[acyl-carrier protein] reductase (fragment) from Brassica napus (Rape) (317 aa), FASTA scores: opt: 447, E(): 2.4e-18, (39.2% identity in 255 aa overlap); etc. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. O53547 No intermediary metabolism and respiration O53547 O53547 GO:0055114,GO:0016491 1.-.-.- O53547 NC_000962.3 Mycobrowser_v5 CDS 3923698 3924819 . + 0 Rv3505 fadE27 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE27 Rv3505, (MTV023.12), len: 373 aa. Probable fadE27, acyl-CoA dehydrogenase, similar to other acyl-CoA dehydrogenases from Mycobacterium tuberculosis e.g. P71857|FADE28|Rv3544c|MTCY03C7.12 (339 aa) FASTA scores: opt: 497, E(): 1.8e-22, (30.3% identity in 343 aa overlap); and P95281|FADE18|Rv1933c|MTCY09F9.31 (363 aa) FASTA scores: opt: 421, E(): 6.4e-18, (32.35% identity in 334 aa overlap). Also similar to other e.g. Q9A5G8|CC2479 from Caulobacter crescentus (344 aa), FASTA scores: opt: 425, E(): 3.5e-18, (30.75% identity in 351 aa overlap); Q9RJX3|SCF37.28c from Streptomyces coelicolor (362 aa) FASTA scores: opt: 317, E(): 1e-11, (32.8% identity in 372 aa overlap); Q9L8Q3|PDTORFO from Pseudomonas stutzeri (Pseudomonas perfectomarina) (513 aa), FASTA scores: opt: 301, E(): 1.2e-10, (25.9% identity in 394 aa overlap); etc. Could belong to the acyl-CoA dehydrogenases family. I6Y3Q0 No lipid metabolism O53550 I6Y3Q0 GO:0003995,GO:0055114,GO:0050660 1.3.99.- I6Y3Q0 NC_000962.3 Mycobrowser_v5 CDS 3924890 3926398 . + 0 Rv3506 fadD17 Function unknown, but supposed involvement in lipid degradation. Fatty-acid-CoA synthetase FadD17 (fatty-acid-CoA synthase) (fatty-acid-CoA ligase) Rv3506, (MTV023.13), len: 502 aa. fadD17, fatty-acid-CoA synthetase (ligase), similar to P72007|FADD1|RV1750c|MTCY28.13c|MTCY04C12.34 from Mycobacterium tuberculosis (532 aa), FASTA scores: opt: 666, E(): 9.8e-32, (52.05% identity in 488 aa overlap). Also similar to various ligases/synthetases e.g. Q9EY88|FCS feruloyl-CoA synthetase from Amycolatopsis sp. HR167 (491 aa), FASTA scores: opt: 490, E(): 2.1e-21, (30.3% identity in 462 aa overlap); BAB33463|ECS0040 (alias AAG54340|CAIC) probable crotonobetaine/carnitine-CoA ligase from Escherichia coli strain O157:H7 (522 aa), FASTA scores: opt: 478, E(): 1.1e-20, (28.5% identity in 347 aa overlap); Q9KHL1|ENCH putative acyl-CoA ligase from Streptomyces maritimus (535 aa), FASTA scores: opt: 477, E(): 1.3e-20, (28.7% identity in 453 aa overlap); Q50017|XCLC|ML1051 acyl-CoA synthase from Mycobacterium leprae (476 aa), FASTA scores: opt: 472, E(): 2.3e-20, (31.35% identity in 469 aa overlap); P31552|CAIC_ECOLI|B0037 from Escherichia coli strain K12 (522 aa), FASTA scores: opt: 467, E(): 4.8e-20, (28.75% identity in 348 aa overlap); Q9KBC2|BH2006 from Bacillus halodurans long-chain acyl-CoA synthetase (ligase) (513 aa), FASTA scores: opt: 462, E(): 9.4e-20, (27.65% identity in 463 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. O53551 No lipid metabolism O53551 O53551 GO:0008152,GO:0016874 6.2.1.- O53551 NC_000962.3 Mycobrowser_v5 CDS 3958448 3959512 . + 0 Rv3522 ltp4 Function unknown; probably involved in lipid metabolism. Possible lipid transfer protein or keto acyl-CoA thiolase Ltp4 Rv3522, (MTV023.29), len: 354 aa. Possible ltp4, lipid carrier protein or keto acyl-CoA thiolase, similar to several e.g. O30103|AF0134 3-ketoacyl-CoA thiolase (ACAB-4) from Archaeoglobus fulgidus (398 aa) FASTA scores: opt: 352, E(): 5.3e-15, (30.45% identity in 381 aa overlap); O29295|AF0967 3-ketoacyl-CoA thiolase (ACAB-9) from Archaeoglobus fulgidus (400 aa) FASTA scores: opt: 312, E(): 1.8e-12, (28.05% identity in 367 aa overlap); O29294|AF0968 3-ketoacyl-CoA thiolase (ACAB-10) from Archaeoglobus fulgidus (388 aa), FASTA scores: opt: 293, E(): 2.9e-11, (25.9% identity in 309 aa overlap); O58409|PH0676 long hypothetical nonspecific lipid-transfer protein (acethyl CoA synthetase) from Pyrococcus horikoshii (389 aa), FASTA scores: opt: 292, E(): 3.3e-11, (25.8% identity in 368 aa overlap); Q9Y9A3|APE2382 long hypothetical non specific lipid-transfer protein from Aeropyrum pernix (360 aa) FASTA scores: opt: 270, E(): 7.8e-10, (27.25% identity in 363 aa overlap); Q9YDI4|APE0929 long hypothetical nonspecific lipid-transfer protein from Aeropyrum pernix (400 aa), FASTA scores: opt: 258, E(): 4.9e-09, (26.45% identity in 306 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). O53567 No lipid metabolism O53567 GO:0008152,GO:0003988 2.3.1.16 O53567 NC_000962.3 Mycobrowser_v5 CDS 3936877 3938424 . - 0 Rv3509c ilvX Could be involved in valine and isoleucine biosynthesis (at the first step) [catalytic activity: 2-acetolactate + CO(2) = 2 pyruvate]. Probable acetohydroxyacid synthase IlvX (acetolactate synthase) Rv3509c, (MTV023.16), len: 515 aa. Probable ilvX, acetohydroxyacid synthase, equivalent to Mycobacterium leprae protein described as Acetolactate synthase I, valine sensitive, large subunit Q49865|ILVX|ILVI1|B229_C3_222 (515 aa), FASTA scores: opt: 2762, E(): 8.8e-145, (82.9% identity in 515 aa overlap). Also similar to various enzymes (principally acetohydroxyacid/acetolactate synthases) e.g. Q9AB41|CC0393 thiamine-pyrophosphate-requiring enzyme from Caulobacter crescentus (512 aa), FASTA scores: opt: 1572, E(): 2.8e-79, (50.95% identity in 514 aa overlap); BAB50432|MLL3567 acetolactate synthase I from Rhizobium loti (Mesorhizobium loti) (517 aa), FASTA scores: opt: 1440, E(): 5.2e-72, (47.9% identity in 548 aa overlap); P20906|MDLC_PSEPU benzoylformate decarboxylase from Pseudomonas putida (528 aa), FASTA scores: opt: 356, E(): 2.5e-12, (28.1% identity in 530 aa overlap); Q9L123|SC6D11.33c putative decarboxylase from Streptomyces coelicolor (526 aa), FASTA scores: opt: 325, E(): 1.3e-10, (33.2% identity in 530 aa overlap); Q9RDF9|SCC57A.40c putative acetolactate synthase from Streptomyces coelicolor (564 aa), FASTA scores: opt: 304, E(): 1.9e-09, (28.55% identity in 550 aa overlap); P94783 valine-sensitive acetohydroxy acid synthase from Citrobacter freundii (561 aa), FASTA scores: opt: 278, E(): 5.1e-08, (25.8% identity in 550 aa overlap); Q42767|AHAS acetohydroxyacid synthase from Gossypium hirsutum (Upland cotton) (659 aa), FASTA scores: opt: 278, E(): 5.8e-08, (26.15% identity in 558 aa overlap); etc. Note that other Mycobacterium tuberculosis proteins, e.g. O53250|MTV012.17c|ILVB_MYCTU|Rv3003c|MT3083|MTV012.17c, show better similarity to Acetolactate synthase I. Similar to other enzymes which require TPP. Cofactor: thiamin pyrophosphate (by similarity). O53554 No intermediary metabolism and respiration O53554 O53554 GO:0030976,GO:0003984 2.2.1.6 O53554 NC_000962.3 Mycobrowser_v5 CDS 3941724 3944963 . + 0 Rv3512 PE_PGRS56 Function unknown PE-PGRS family protein PE_PGRS56 Rv3512, (MTV023.19), len: 1079 aa. PE_PGRS56, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), similar to others from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. AAK47974|MT3615.3 (1217 aa) FASTA scores: opt: 3688, E(): 4.5e-130, (53.95% identity in 1136 aa overlap); and downstream O53559|Rv3514|MTV023.21 (1489 aa), FASTA scores: opt: 3611, E(): 3.6e-127, (53.15% identity in 1195 aa overlap); etc. Frameshifted PGRS protein, could be continuation of upstream MTV023.18, but no error could be found. Q6MWW7 No PE/PPE Q6MWW7 NC_000962.3 Mycobrowser_v5 CDS 3959529 3960713 . + 0 Rv3523 ltp3 Function unknown; probably involved in lipid metabolism. Probable lipid carrier protein or keto acyl-CoA thiolase Ltp3 Rv3523, (MTCY03C7.33c), len: 394 aa. Probable ltp3, lipid carrier protein or keto acyl-CoA thiolase, similar to several e.g. O30037|AF0202 3-ketoacyl-CoA thiolase (ACAB-6) from Archaeoglobus fulgidus (380 aa) FASTA scores: opt: 782, E(): 1.7e-40, (38.35% identity in 386 aa overlap); Q9Y9A1|APE2384 long hypothetical non specific lipid-transfer protein (acethyl CoA synthetase) from Aeropyrum pernix (394 aa), FASTA scores: opt: 626, E(): 5.9e-31, (35.75% identity in 386 aa overlap); BAB59210|TVG0067506 lipid transfer protein from Thermoplasma volcanium (390 aa), FASTA scores: opt: 591, E(): 8.1e-29, (34.35% identity in 384 aa overlap); Q9YDI4|APE0929 long hypothetical nonspecific lipid-transfer protein from Aeropyrum pernix (400 aa) FASTA scores: opt: 588, E(): 1.3e-28, (31.6% identity in 408 aa overlap); O30104|AF0133 3-ketoacyl-CoA thiolase (ACAB-3) from Archaeoglobus fulgidus (411 aa) FASTA scores: opt: 583, E(): 2.6e-28, (39.8% identity in 412 aa overlap); O29811|AF0438 3-ketoacyl-CoA thiolase (ACAB-8) from Archaeoglobus fulgidus (387 aa), FASTA scores: opt: 574, E(): 8.8e-28, (30.95% identity in 388 aa overlap); etc. I6YGD8 No lipid metabolism I6YGD8 GO:0008152,GO:0003988 2.3.1.16 I6YGD8 NC_000962.3 Mycobrowser_v5 CDS 3962439 3963599 . + 0 Rv3526 kshA Predicted to be involved in lipid catabolism Oxygenase component of 3-ketosteroid-9-alpha-hydroxylase KshA Rv3526, (MTCY03C7.30c), len: 386 aa. kshA, oxygenase component of 3-ketosteroid-9-alpha-hydroxylase, highly similar, except in C-terminus (also longer 69 aa), to O69348|ORF12 protein (function unknown) from Rhodococcus erythropolis (316 aa) FASTA scores: opt: 1137, E(): 6.9e-65, (59.6% identity in 250 aa overlap). Also some similarity with several aminopyrrolnitrin oxidases (PRND proteins, involved in the pathway for pyrrolnitrin biosynthesis, a secondary metabolite derived from tryptophan which has strong anti-fungal activity) e.g. Q9RPG0|PRND from Myxococcus fulvus (379 aa), FASTA scores: opt: 322, E(): 4.4e-13, (25.85% identity in 352 aa overlap); Q9RPG4|PRND from Burkholderia cepacia (Pseudomonas cepacia) (373 aa) FASTA scores: opt: 306, E(): 4.5e-12, (25.2% identity in 373 aa overlap); P95483|PRND from Pseudomonas fluorescens (363 aa), FASTA scores: opt: 305, E(): 5.1e-12, (25.0% identity in 372 aa overlap); etc. And also some similarity to other putative enzymes like dioxygenases, oxidases, vanillate O-demethyl oxygenase, etc. P71875 No intermediary metabolism and respiration 2ZYL P71875 P71875 GO:0046872,GO:0055114,GO:0016491,GO:0051537 1.-.-.- P71875 NC_000962.3 Mycobrowser_v5 CDS 3972552 3973592 . - 0 Rv3534c hsaF Supposed involvement in one, or several, catabolic pathways [catalytic activity: 4-hydroxy-2-oxovalerate = pyruvate + acetaldehyde]. Predicted to be involved in lipid catabolism. Probable 4-hydroxy-2-oxovalerate aldolase (HOA) Rv3534c, (MTCY03C7.22), len: 346 aa. Probable hsaF, 4-hydroxy-2-oxovalerate aldolase, highly similar to others e.g. P51015|BPHI_PSESP from Pseudomonas sp. strain LB400 (346 aa), FASTA scores: opt: 1150, E(): 2.3e-61, (51.35% identity in 331 aa overlap); Q52040|BPHX3 from Pseudomonas pseudoalcaligenes (346 aa), FASTA scores: opt: 1147, E(): 3.5e-61, (51.35% identity in 331 aa overlap); P51017|NAHM_PSEPU from Pseudomonas putida (346 aa), FASTA scores: opt: 1145, E(): 4.7e-61, (50.9% identity in 330 aa overlap) (see citation below); P51020|MHPE_ECOLI|MHPF|B0352 from Escherichia coli strain K12 (337 aa), FASTA scores: opt: 1133, E(): 2.4e-60, (52.0% identity in 327 aa overlap); O24833|ATDG from Acinetobacter sp (340 aa), FASTA scores: opt: 1132, E(): 2.7e-60, (50.45% identity in 331 aa overlap); etc. Note that also highly similar to Q9ZI56|NAHM 2-oxo-4-hydroxypentanoate aldolase from Pseudomonas stutzeri (Pseudomonas perfectomarina) (346 aa) FASTA scores: opt: 1168, E(): 2e-62, (51.05% identity in 331 aa overlap) (see citation below). P9WMK5 No intermediary metabolism and respiration P71867 P9WMK5 GO:0019439,GO:0008701 4.1.3.39 P9WMK5 NC_000962.3 Mycobrowser_v5 CDS 3974511 3975296 . - 0 Rv3536c hsaE Function unknown; probably involved in cellular metabolism. Predicted to be involved in lipid catabolism. Probable hydratase Rv3536c, (MTCY03C7.20), len: 261 aa. Probable hsaE, hydratase, 2-oxo-hepta-3-ene-1,7-dioate hydratase or 2-keto-4-pentenoate hydratase. Indeed, highly similar to many 2-oxo-hepta-3-ene-1,7-dioate hydratases e.g. Q9CKS2|HPAH|PM1534 from Pasteurella multocida (267 aa) FASTA scores: opt: 743, E(): 1.5e-39, (45.5% identity in 266 aa overlap) Q9RZ31|DRA0122 from Deinococcus radiodurans (268 aa), FASTA scores: opt: 709, E(): 2e-37, (45.5% identity in 266 aa overlap); Q9HWQ4|HPCG|PA4127 from Pseudomonas aeruginosa (267 aa), FASTA scores: opt: 703, E(): 4.8e-37, (45.1% identity in 266 aa overlap); Q46982|HPAH|HPCG from Escherichia colis strain ATCC 11105 (267 aa), FASTA scores: opt: 679, E(): 1.6e-35, (41.35% identity in 266 aa overlap); etc. But also highly similar to many 2-keto-4-pentenoate hydratases (2-hydroxypentadienoic acidhydratases) e.g. Q9LAF7|PHED from Bacillus thermoglucosidasius (258 aa), FASTA scores: opt: 698, E(): 9.7e-37, (42.45% identity in 252 aa overlap); Q52442|BPHH from Pseudomonas sp (260 aa) FASTA scores: opt: 675, E(): 2.7e-35, (41.4% identity in 251 aa overlap); P77608|MHPD_ECOLI|B0350 from Escherichia coli strain K12 (269 aa), FASTA scores: opt: 674, E(): 3.2e-35, (42.75% identity in 255 aa overlap); Q52038|BPHX1 from Pseudomonas pseudoalcaligenes (260 aa), FASTA scores: opt: 663, E(): 1.5e-34, (40.6% identity in 251 aa overlap); etc. I6XHH5 No intermediary metabolism and respiration P71865 I6XHH5 GO:0008152,GO:0016829 4.2.1.- I6XHH5 NC_000962.3 Mycobrowser_v5 CDS 3977062 3977922 . + 0 Rv3538 Rv3538 Unknown; probably involved in cellular metabolism. Predicted to be involved in lipid catabolism. Probable dehydrogenase. Possible 2-enoyl acyl-CoA hydratase. Rv3538, (MTCY03C7.18c), len: 286 aa. Probable double hotdog R-specific hydratase, substrate unknown, shows structural similarity to six others in Mycobacterium tuberculosis (see Castell et al (2005) below) especially Rv3389. Probable dehydrogenase, similar to Q9L009|SCC30.12c putative dehydrogenase from Streptomyces coelicolor (333 aa), FASTA scores: opt: 842, E(): 3.6e-44, (48.4% identity in 285 aa overlap); and similar to C-terminal part of other (principally estradiol 17 beta-dehydrogenases/17-beta-hydroxysteroid dehydrogenases) e.g. P70540 peroxisomal multifunctional enzyme type II (SDR family) from Rattus norvegicus (Rat) (735 aa) FASTA scores: opt: 622, E(): 1.9e-30, (37.45% identity in 283 aa overlap); or P70523|MPF-2 multifunctional protein 2 (SDR family) (beta-oxidation protein displaying 2-enoyl-CoA hydratase and D-3-hydroxyacyl-CoA dehydrogenase activity) from Rattus norvegicus (Rat) (734 aa), FASTA scores: opt: 616, E(): 4.3e-30, (37.1% identity in 283 aa overlap); P51659|DHB4_HUMAN|HSD17B4|EDH17B4 estradiol 17 beta-dehydrogenase from Homo sapiens (Human) (736 aa), FASTA scores: opt: 614, E(): 5.7e-30, (35.9% identity in 284 aa overlap); P97852|DHB4_RAT|HSD17B4|EDH17B4 estradiol 17 beta-dehydrogenase from Rattus norvegicus (Rat) (735 aa) FASTA scores: opt: 613, E(): 6.6e-30, (37.1% identity in 283 aa overlap); Q9DBM3|HSD17B4 estradiol 17 beta-dehydrogenase from Mus musculus (Mouse) (735 aa) FASTA scores: opt: 611, E(): 8.7e-30, (36.5% identity in 285 aa overlap); etc. Also similar to Q11198|Rv3389c|MTV004.47c hypothetical 30.3 KDA protein from Mycobacterium tuberculosis (290 aa), FASTA scores: opt: 609, E(): 5.3e-30, (39.65% identity in 285 aa overlap). Note that previously known as ufaA2. Q6MWW2 No intermediary metabolism and respiration Q6MWW2 Q6MWW2 GO:0016491,GO:0055114 1.-.-.- Q6MWW2 NC_000962.3 Mycobrowser_v5 CDS 3981977 3983140 . - 0 Rv3543c fadE29 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE29 Rv3543c, (MTCY03C7.13), len: 387 aa. Probable fadE29, acyl-CoA dehydrogenase, similar to many e.g. Q9A8P3|CC1310 from Caulobacter crescentus (404 aa), FASTA scores: opt: 624, E(): 9.4e-32, (32.75% identity in 400 aa overlap); Q9I4V2|PA1022 from Pseudomonas aeruginosa (381 aa), FASTA scores: opt: 550, E(): 3.9e-27, (33.7% identity in 350 aa overlap); O28976|AF1293 from Archaeoglobus fulgidus (384 aa), FASTA scores: opt: 529, E(): 8.1e-26, (30.0% identity in 393 aa overlap); etc. Also similar to other from Mycobacterium tuberculosis e.g. O53549|FADE26|Rv3504|MTV023.11 (400 aa), FASTA scores: opt: 1031, E(): 2.8e-57, (46.0% identity in 402 aa overlap). Could belong to the acyl-CoA dehydrogenases family. P71858 No lipid metabolism P71858 P71858 GO:0003995,GO:0055114,GO:0050660 1.3.99.- P71858 NC_000962.3 Mycobrowser_v5 CDS 3991621 3992688 . + 0 Rv3553 Rv3553 Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv3553, (MTCY03C7.02c), len: 355 aa. Possible oxidoreductase, highly similar (except in C-terminus) to Q9A327|CC3379 hypothetical protein from Caulobacter crescentus (321 aa), FASTA scores: opt: 639, E(): 4.6e-29, (46.35% identity in 248 aa overlap); and Q9WZQ7|TM0800 conserved hypothetical protein from Thermotoga maritima (314 aa), FASTA scores: opt: 622, E(): 4.1e-28, (37.95% identity in 340 aa overlap). Also similar to two trans-2-enoyl-ACP reductases; Q99YD4|FABK|SPY1751 from Streptococcus pyogenes (323 aa), FASTA scores: opt: 604, E(): 4.4e-27, (33.25% identity in 346 aa overlap); and Q9FBC5|FABK from Streptococcus pneumoniae (324 aa), FASTA scores: opt: 553, E(): 3.3e-24, (32.1% identity in 346 aa overlap); and similar with several 2-nitropropane dioxygenases, e.g. Q9F7P8 from uncultured proteobacterium EBAC31A08 (322 aa), FASTA scores: opt: 505, E(): 1.7e-21, (33.6% identity in 348 aa overlap); Q9FMG0 (alias AAK44141) from Arabidopsis thaliana (Mouse-ear cress) (333 aa), FASTA scores: opt: 489, E(): 1.4e-20, (33.15% identity in 341 aa overlap); O28109|AF2173 (NCD2) from Archaeoglobus fulgidus (274 aa), FASTA scores: opt: 456, E(): 8.9e-19, (36.3% identity in 237 aa overlap); etc. P71847 No intermediary metabolism and respiration P71847 P71847 GO:0055114,GO:0018580 1.-.-.- P71847 NC_000962.3 Mycobrowser_v5 CDS 3983125 3984144 . - 0 Rv3544c fadE28 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE28 Rv3544c, (MTCY03C7.12), len: 339 aa. Probable fadE28, acyl-CoA dehydrogenase, similar to many e.g. Q9RJX3|SCF37.28c from Streptomyces coelicolor (362 aa), FASTA scores: opt: 334, E(): 5.1e-13, (27.65% identity in 329 aa overlap); Q9A5G8|CC2479 from Caulobacter crescentus (344 aa), FASTA scores: opt: 278, E(): 1.2e-09, (26.95% identity in 319 aa overlap); O29813|AF0436 from Archaeoglobus fulgidus (382 aa) FASTA scores: opt: 205, E(): 3.5e-05, (24.75% identity in 384 aa overlap); etc. Also similar to other from Mycobacterium tuberculosis e.g. O53550|FADE27|Rv3505|MTV023.12 (373 aa) FASTA scores: opt: 497, E(): 7e-23, (30.3% identity in 343 aa overlap); and to P46703|ACDP_MYCLE|FADE25|ACD|ML0737|B1308_F1_34 probable acyl-CoA dehydrogenase from Mycobacterium leprae (389 aa) FASTA scores: opt: 165, E(): 0.0012, (25.2% identity in 345 aa overlap). Could belong to the acyl-CoA dehydrogenases family. P71857 No lipid metabolism P71857 P71857 GO:0003995,GO:0055114,GO:0050660 1.3.99.- P71857 NC_000962.3 Mycobrowser_v5 CDS 3984144 3985445 . - 0 Rv3545c cyp125 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Probable cytochrome P450 125 Cyp125 Rv3545c, (MT3649, MTCY03C7.11), len: 433 aa. Probable cyp125, cytochrome P-450, similar to others e.g. Q59723|LINC|CYP111 from Pseudomonas incognita (406 aa), FASTA scores: opt: 831, E(): 8e-45, (34.75% identity in 406 aa overlap); Q9X8Q3|CYP107P1|SCH10.14c from Streptomyces coelicolor (411 aa), FASTA scores: opt: 694, E(): 3.3e-36, (32.35% identity in 417 aa overlap); Q9L465|CYP162A1|NIKQ from Streptomyces tendae (396 aa) FASTA scores: opt: 664, E(): 2.5e-34, (34.15% identity in 413 aa overlap); O08469|CPXY_BACSU|CYPA|CYP107J1 from Bacillus subtilis (410 aa), FASTA scores: opt: 579, E(): 5.6e-29, (30.05% identity in 366 aa overlap); etc. Also similar to other from Mycobacterium tuberculosis e.g. Q50696|CYP124|Rv2266|MT2328|MTCY339.44c (428 aa) FASTA scores: opt: 1040, E(): 6.1e-58, (40.75% identity in 432 aa overlap). Belongs to the cytochrome P450 family. P9WPP1 No intermediary metabolism and respiration 3IVY,3IW0,3IW1,3IW2,2X5L,2X5W,2XC3,2XN8 P63709 P9WPP1 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPP1 NC_000962.3 Mycobrowser_v5 CDS 3994830 3995699 . - 0 Rv3555c Rv3555c Function unknown Conserved protein Rv3555c, (MTCY06G11.02c), len: 289 aa. Conserved protein, highly similar to others from Mycobacterium tuberculosis e.g. O53562|AL022022|Rv3517|MTV023.24 (279 aa), FASTA scores: opt: 874, E(): 8.3e-48, (49.45% identity in 275 aa overlap); P71763|Rv1482c|MTCY277.03c (339 aa), FASTA scores: opt: 755, E(): 3e-40, (45.75% identity in 260 aa overlap); O69681|Rv3714c|MTV025.062c (296 aa), FASTA scores: opt: 733, E(): 6.4e-39, (44.1% identity in 281 aa overlap); etc. Also highly similar to other mycobacterial hypothetical proteins e.g. O07396|MAV346 from Mycobacterium avium (346 aa), FASTA scores: opt: 714, E(): 1.1e-37, (44.6% identity in 260 aa overlap); and Q50134|U650AG|MLCB57.67c from Mycobacterium leprae (75 aa), FASTA scores: opt: 130, E(): 0.17, (35.1% identity in 57 aa overlap) (only partial homology with this protein). Shows some similarity to P52392|NHSR_STRAS putative nosiheptide resistance regulatory protein (ORF699) from Streptomyces actuosus (233 aa), FASTA scores: opt: 120, E(): 1.9, (25.25% identity in 194 aa overlap). P96837 No conserved hypotheticals P96837 P96837 GO:0003677 P96837 NC_000962.3 Mycobrowser_v5 CDS 3995804 3996964 . - 0 Rv3556c fadA6 Function unknown, but involved in lipid degradation [catalytic activity: 2 acetyl-CoA = CoA + acetoacetyl-CoA]. Probable acetyl-CoA acetyltransferase FadA6 (acetoacetyl-CoA thiolase) Rv3556c, (MTCY06G11.03), len: 386 aa. Probable fadA6, acetyl-CoA acetyltransferase, similar to many e.g. Q9K409|2SCG61.06c from Streptomyces coelicolor (389 aa), FASTA scores: opt: 1091, E(): 2.9e-58, (48.1% identity in 399 aa overlap); Q9AAT4|CC0510 from Caulobacter crescentus (391 aa), FASTA scores: opt: 902, E(): 6.6e-47, (40.25% identity in 395 aa overlap); P45359|THL_CLOAB from Clostridium acetobutylicum (392 aa), FASTA scores: opt: 872, E(): 4.2e-45, (37.9% identity in 396 aa overlap); Q9I2A8|ATOB|PA2001 from Pseudomonas aeruginosa (393 aa), FASTA scores: opt: 872, E(): 4.2e-45, (41.3% identity in 397 aa overlap); etc. Contains PS00737 Thiolases signature 2. Belongs to the thiolase family. I6XHJ3 No lipid metabolism P96838 I6XHJ3 GO:0008152,GO:0003985 2.3.1.9 I6XHJ3 NC_000962.3 Mycobrowser_v5 CDS 3997980 3999638 . + 0 Rv3558 PPE64 Function unknown PPE family protein PPE64 Rv3558, (MTCY06G11.05), len: 552 aa. PPE64, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, similar to many e.g. P71868|Rv3533c|MTCY03C7.23 (582 aa), FASTA scores: opt: 1908, E(): 1.7e-83, (58.5% identity in 583 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). Q6MWW0 No PE/PPE Q6MWW0 Q6MWW0 Q6MWW0 NC_000962.3 Mycobrowser_v5 CDS 4001637 4003160 . + 0 Rv3561 fadD3 Function unknown, but involved in lipid degradation. Probable fatty-acid-CoA ligase FadD3 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv3561, (MTCY06G11.08), len: 507 aa. Probable fadD3, fatty-acid-CoA synthetase, similar to many substrate-CoA symthetases/ligases e.g. Q9KBC2|BH2006 long-chain acyl-CoA synthetase from Bacillus halodurans (513 aa), FASTA scores: opt: 821, E(): 1.6e-43, (32.9% identity in 517 aa overlap); Q9EY88|FCS feruloyl-CoA synthetase from Amycolatopsis sp. HR167 (491 aa) FASTA scores: opt: 767, E(): 3.5e-40, (37.65% identity in 502 aa overlap); Q9ZIP5|MATB malonyl CoA synthetase from Rhizobium leguminosarum (504 aa), FASTA scores: opt: 758, E(): 1.3e-39, (33.7% identity in 472 aa overlap); Q9CD27|FADD2|ML2546 acyl-CoA synthase from Mycobacterium leprae (548 aa), FASTA scores: opt: 700, E(): 5.6e-36, (31.85% identity in 515 aa overlap); P29212|LCFA_ECOLI|FADD|OLDD|B1805 long-chain-fatty-acid--CoA ligase from Escherichia coli strain K12 (561 aa), FASTA scores: opt: 532, E(): 6.3e-28, (30.0% identity in 533 aa overlap); etc. Also similar to other from Mycobacterium tuberculosis eg O53306|FADD13|Rv3089|MTV013.10 (503 aa), FASTA scores: opt: 819, E(): 2.1e-43, (35.1% identity in 490 aa overlap). Contains PS00455 Putative AMP-binding domain signature. P96843 No lipid metabolism P96843 P96843 GO:0008152,GO:0016874 6.2.1.- P96843 NC_000962.3 Mycobrowser_v5 CDS 4004291 4005250 . + 0 Rv3563 fadE32 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE32 Rv3563, (MTCY06G11.10), len: 319 aa. Probable fadE32, acyl-CoA dehydrogenase, similar to many e.g. Q9I4V4|PA1020 from Pseudomonas aeruginosa (370 aa), FASTA scores: opt: 347, E(): 7.6e-14, (35.15% identity in 333 aa overlap); Q9RJX3|SCF37.28c from Streptomyces coelicolor (362 aa), FASTA scores: opt: 300, E(): 5.3e-11, (32.4% identity in 349 aa overlap); Q9A5G8|CC2479 from Caulobacter crescentus (344 aa), FASTA scores: opt: 285, E(): 4.1e-10, (30.4% identity in 329 aa overlap); P45857|ACDB_BACSU|MMGC from Bacillus subtilis (379 aa), FASTA scores: opt: 230, E(): 1.1e-07, (25.5% identity in 357 aa overlap); etc. Also similar to other from Mycobacterium tuberculosis eg P96846|FADE33|Rv3564|MTCY06G11.11 (318 aa), FASTA scores: opt: 478, E(): 7.6e-22, (32.9% identity in 292 aa overlap). Could belong to the acyl-CoA dehydrogenases family. P96845 No lipid metabolism P96845 P96845 GO:0003995,GO:0055114,GO:0050660 1.3.99.- P96845 NC_000962.3 Mycobrowser_v5 CDS 4005247 4006203 . + 0 Rv3564 fadE33 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE33 Rv3564, (MTCY06G11.11), len: 318 aa. Probable fadE33, acyl-CoA dehydrogenase, similar to others e.g. Q9A5G8|CC2479 from Caulobacter crescentus (344 aa), FASTA scores: opt: 373, E(): 1.9e-15, (34.3% identity in 338 aa overlap); Q9I4V4|PA1020 from Pseudomonas aeruginosa (370 aa), FASTA scores: opt: 277, E(): 1.4e-09, (31.95% identity in 335 aa overlap); Q9X7Y6|SC6A5.40c from Streptomyces coelicolor (395 aa), FASTA scores: opt: 273, E(): 2.5e-09, (30.1% identity in 352 aa overlap); P45857|ACDB_BACSU|MMGC from Bacillus subtilis (379 aa), FASTA scores: opt: 478, E(): 7.9e-22, (32.9% identity in 292 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. P96845|FADE32|Rv3563|MTCY06G11.10 (319 aa), FASTA scores: opt: 478, E(): 7.9e-22, (32.9% identity in 292 aa overlap). Could belong to the acyl-CoA dehydrogenases family. I6YCF5 No lipid metabolism P96846 I6YCF5 GO:0003995,GO:0055114,GO:0050660 1.3.99.- I6YCF5 NC_000962.3 Mycobrowser_v5 CDS 4006200 4007366 . + 0 Rv3565 aspB Thought to be involved in glutamate biosynthesis [catalytic activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate]. Possible aspartate aminotransferase AspB (transaminase A) (ASPAT) (glutamic--oxaloacetic transaminase) (glutamic--aspartic transaminase) Rv3565, (MTCY06G11.12), len: 388 aa. Possible aspB, aspartate aminotransferase, similar to many e.g. Q9A5J2|CC2455 aminotransferase class I from Caulobacter crescentus (381 aa), FASTA scores: opt: 1112, E(): 1e-61, (45.85% identity in 384 aa overlap); Q9HV76|PA4722 probable aminotransferase from Pseudomonas aeruginosa (390 aa), FASTA scores: opt: 863, E(): 3.1e-46, (37.2% identity in 390 aa overlap); Q9RWP3|DR0623 aspartate aminotransferase from Deinococcus radiodurans (388 aa), FASTA scores: opt: 713, E(): 6.3e-37, (35.5% identity in 383 aa overlap); Q9HQK2|ASPC2|VNG1121G aspartate aminotransferase from Halobacterium sp. strain NRC-1 (391 aa), FASTA scores: opt: 710, E(): 9.8e-37, (34.45% identity in 380 aa overlap); O33822|AAT_THEAQ|ASPC aspartate aminotransferase from Thermus aquaticus (383 aa), FASTA scores: opt: 695, E(): 8.2e-36, (35.1% identity in 376 aa overlap); etc. Contains PS00105 Aminotransferases class-I pyridoxal-phosphate attachment site. Belongs to class-I of pyridoxal-phosphate-dependent aminotransferases. Cofactor: pyridoxal phosphate (by similarity). P96847 No intermediary metabolism and respiration P96847 P96847 GO:0004069,GO:0009058,GO:0030170,GO:0016847 2.6.1.1 P96847 NC_000962.3 Mycobrowser_v5 CDS 4010196 4011071 . - 0 Rv3569c hsaD Catalyzes the hydrolysis of 4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-OIC acid (4,9-DHSA) 4,9-DHSA hydrolase Rv3569c, (MTCY06G11.16c), len: 291 aa. HsaD, highly similar to e.g. Q9KWQ6|BPHD2 from Rhodococcus sp. RHA1 (292 aa), FASTA scores: opt: 1468, E(): 1.3e-85, (75.5% identity in 294 aa overlap); Q52036 from Pseudomonas putida (286 aa), FASTA scores: opt: 785, E(): 1.9e-42, (45.1% identity in 295 aa overlap); Q52011|BPHD from Pseudomonas pseudoalcaligenes (286 aa), FASTA scores: opt: 774, E(): 9.3e-42, (44.05% identity in 295 aa overlap); P47229|BPHD_BURCE from Burkholderia cepacia (Pseudomonas cepacia) (286 aa) FASTA scores: opt: 772, E(): 1.2e-41, (44.5% identity in 295 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A. Similar to alpha/beta hydrolase fold. P9WNH5 No intermediary metabolism and respiration 2VF2,2WUD,2WUE,2WUF,2WUG P96851 P9WNH5 GO:0016787 3.7.1.- P9WNH5 NC_000962.3 Mycobrowser_v5 promoter 4012356 4012362 . + 0 Rv3571 kshB Predicted to be involved in lipid catabolism Reductase component of 3-ketosteroid-9-alpha-hydroxylase KshB Rv3571, (MTCY06G11.18), len: 358 aa. kshB, reductase component of 3-ketosteroid-9-alpha-hydroxylase, similar to several e.g. Q44253|ATDA5 aniline dioxygenase reductase component from Acinetobacter sp (336 aa) FASTA scores: opt: 748, E(): 1.5e-38, (34.95% identity in 346 aa overlap); P95533|TDNB electron transfer protein from Pseudomonas putida (337 aa), FASTA scores: opt: 723, E(): 5.2e-37, (36.35% identity in 341 aa overlap); AAK65059|SMA0752 possible dioxygenase reductase subunit from Rhizobium meliloti (Sinorhizobium meliloti) (353 aa) FASTA scores: opt: 495, E(): 4.9e-23, (31.9% identity in 345 aa overlap); P76081|PAAE_ECOLI|B1392 probable phenylacetic acid degradation NADH oxidoreductase (356 aa), FASTA scores: opt: 364, E(): 5.1e-15, (34.45% identity in 357 aa overlap); Q9L131|HMPA flavohemoprotein from Streptomyces coelicolor (398 aa), FASTA scores: opt: 352, E(): 3e-14, (32.8% identity in 247 aa overlap); etc. Contains PS00197 2Fe-2S ferredoxins, iron-sulfur binding region signature. Note that it has been shown hmp transcription increased at early stationary phase and is lower at late stationary phase and during exponential growth. Note that previously known as hmp. P9WJ93 No intermediary metabolism and respiration P96853 P9WJ93 GO:0009055,GO:0046872,GO:0055114,GO:0016491,GO:0051537 1.-.-.- P9WJ93 NC_000962.3 Mycobrowser_v5 promoter 4012380 4012385 . + 0 Rv3571 kshB Predicted to be involved in lipid catabolism Reductase component of 3-ketosteroid-9-alpha-hydroxylase KshB Rv3571, (MTCY06G11.18), len: 358 aa. kshB, reductase component of 3-ketosteroid-9-alpha-hydroxylase, similar to several e.g. Q44253|ATDA5 aniline dioxygenase reductase component from Acinetobacter sp (336 aa) FASTA scores: opt: 748, E(): 1.5e-38, (34.95% identity in 346 aa overlap); P95533|TDNB electron transfer protein from Pseudomonas putida (337 aa), FASTA scores: opt: 723, E(): 5.2e-37, (36.35% identity in 341 aa overlap); AAK65059|SMA0752 possible dioxygenase reductase subunit from Rhizobium meliloti (Sinorhizobium meliloti) (353 aa) FASTA scores: opt: 495, E(): 4.9e-23, (31.9% identity in 345 aa overlap); P76081|PAAE_ECOLI|B1392 probable phenylacetic acid degradation NADH oxidoreductase (356 aa), FASTA scores: opt: 364, E(): 5.1e-15, (34.45% identity in 357 aa overlap); Q9L131|HMPA flavohemoprotein from Streptomyces coelicolor (398 aa), FASTA scores: opt: 352, E(): 3e-14, (32.8% identity in 247 aa overlap); etc. Contains PS00197 2Fe-2S ferredoxins, iron-sulfur binding region signature. Note that it has been shown hmp transcription increased at early stationary phase and is lower at late stationary phase and during exponential growth. Note that previously known as hmp. P9WJ93 No intermediary metabolism and respiration P96853 P9WJ93 GO:0009055,GO:0046872,GO:0055114,GO:0016491,GO:0051537 1.-.-.- P9WJ93 NC_000962.3 Mycobrowser_v5 CDS 4012417 4013493 . + 0 Rv3571 kshB Predicted to be involved in lipid catabolism Reductase component of 3-ketosteroid-9-alpha-hydroxylase KshB Rv3571, (MTCY06G11.18), len: 358 aa. kshB, reductase component of 3-ketosteroid-9-alpha-hydroxylase, similar to several e.g. Q44253|ATDA5 aniline dioxygenase reductase component from Acinetobacter sp (336 aa) FASTA scores: opt: 748, E(): 1.5e-38, (34.95% identity in 346 aa overlap); P95533|TDNB electron transfer protein from Pseudomonas putida (337 aa), FASTA scores: opt: 723, E(): 5.2e-37, (36.35% identity in 341 aa overlap); AAK65059|SMA0752 possible dioxygenase reductase subunit from Rhizobium meliloti (Sinorhizobium meliloti) (353 aa) FASTA scores: opt: 495, E(): 4.9e-23, (31.9% identity in 345 aa overlap); P76081|PAAE_ECOLI|B1392 probable phenylacetic acid degradation NADH oxidoreductase (356 aa), FASTA scores: opt: 364, E(): 5.1e-15, (34.45% identity in 357 aa overlap); Q9L131|HMPA flavohemoprotein from Streptomyces coelicolor (398 aa), FASTA scores: opt: 352, E(): 3e-14, (32.8% identity in 247 aa overlap); etc. Contains PS00197 2Fe-2S ferredoxins, iron-sulfur binding region signature. Note that it has been shown hmp transcription increased at early stationary phase and is lower at late stationary phase and during exponential growth. Note that previously known as hmp. P9WJ93 No intermediary metabolism and respiration P96853 P9WJ93 GO:0009055,GO:0046872,GO:0055114,GO:0016491,GO:0051537 1.-.-.- P9WJ93 NC_000962.3 Mycobrowser_v5 CDS 4014077 4016212 . - 0 Rv3573c fadE34 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE34 Rv3573c, (MTCY06G11.20c), len: 711 aa. Probable fadE34, acyl-CoA dehydrogenase, similar to others, especially in C-terminal half, e.g. Q9RJX2|SCF37.29c from Streptomyces coelicolor (393 aa) FASTA scores: opt: 780, E(): 2.8e-39, (44.1% identity in 347 aa overlap); Q9A6N8|CC2049 from Caulobacter crescentus (401 aa), FASTA scores: opt: 705, E(): 8.7e-35, (41.5% identity in 342 aa overlap); Q9EX72|MLHC from Rhodococcus erythropolis (324 aa), FASTA scores: opt: 673, E(): 6.1e-33, (42.05% identity in 283 aa overlap); P41367|ACDM_PIG|ACADM from Sus scrofa (Pig)(421 aa) FASTA scores: opt: 325, E(): 4.9e- 13, (28.5% identity in 368 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. P95097|FADE22|Rv3061c|MTCY22D7.20 (721 aa), FASTA scores: opt: 1635, E(): 2.7e-90, (42.65% identity in 729 aa overlap). Could belong to the acyl-CoA dehydrogenases family. P96855 No lipid metabolism P96855 P96855 GO:0003995,GO:0055114,GO:0050660 1.3.99.- P96855 NC_000962.3 Mycobrowser_v5 CDS 4020142 4021383 . + 0 Rv3578 arsB2 Thought to be involved in transport of arsenic across the membrane (export): arsenic resistance by an export mechanism. Form the channel of an arsenite pump responsible for the translocation of the substrate across the membrane. Possible arsenical pump integral membrane protein ArsB2 Rv3578, (MTCY06G11.25), len: 413 aa. Possible arsB2, arsenical pump integral membrane protein, similar to many e.g. Q9I1J6|ARSB|PA2278 from Pseudomonas aeruginosa (427 aa), FASTA scores: opt: 375, E(): 3.1e-15, (32.15% identity in 429 aa overlap); Q9K8K7|ARSB|BH2999 from Bacillus halodurans (436 aa), FASTA scores: opt: 360, E(): 2.5e-14, (28.7% identity in 432 aa overlap); P52146|ARB2_ECOLI from Escherichia coli (plasmid R46) (429 aa), FASTA scores: opt: 345, E(): 2e-13, (29.8% identity in 426 aa overlap); etc. Also highly similar to Q9KYM0|SC9H11.21c probable membrane efflux protein from Streptomyces coelicolor (446 aa), FASTA scores: opt: 730, E(): 1.7e-36, (53.95% identity in 443 aa overlap). Seems to belong to the ARS family. I6YCG9 No cell wall and cell processes P96860 I6YCG9 GO:0016021,GO:0015105 I6YCG9 NC_000962.3 Mycobrowser_v5 CDS 4023868 4024347 . - 0 Rv3581c ispF Involved in the deoxyxylulose-5-phosphate pathway (DXP) of isoprenoid biosynthesis (at the fifth step). Converts 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate into 2C-methyl-D-erythritol 2,4-cyclodiphosphate and CMP. Also converts 4-diphosphocytidyl-2C-methyl-D-erythritol into 2C-methyl-D-erythritol 3,4-cyclophosphate and CMP. Probable 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase IspF (MECPS) Rv3581c, (MT3687, MTCY06G11.28c), len: 159 aa. Probable ispF, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, equivalent to Q9CCW5|ML0322 putative 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase from Mycobacterium leprae (158 aa), FASTA scores: opt: 830, E(): 2.9e-47, (79.1% identity in 158 aa overlap). Also highly similar to others e.g. Q9L0Q7|ISPF_STRCO|SCD8A.07 from Streptomyces coelicolor (170 aa), FASTA scores: opt: 585, E(): 2.9e-31, (56.5% identity in 154 aa overlap); Q9PDT5|ISPF_XYLFA|XF1294 from Xylella fastidiosa (176 aa), FASTA scores: opt: 398, E(): 4.6e-19, (44.9% identity in 156 aa overlap); Q08113|ISDF_RHOCA|ISPDF from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (379 aa), FASTA scores: opt: 387, E(): 4.5e-18, (42.85% identity in 154 aa overlap) (only similar with C-terminal end of this bifunctional protein ISPD and ISPF); Q06756|ISPF_BACSU from Bacillus subtilis (158 aa), FASTA scores: opt: 367, E(): 4.5e-17, (41.2% identity in 153 aa overlap); etc. Belongs to the IspF family. P9WKG5 No intermediary metabolism and respiration P65183 P9WKG5 GO:0046872,GO:0016114,GO:0008685 4.6.1.12 P9WKG5 NC_000962.3 Mycobrowser_v5 CDS 4024344 4025039 . - 0 Rv3582c ispD Involved in the deoxyxylulose-5-phosphate pathway (DXP) of isoprenoid biosynthesis (at the third step). Catalyzes the formation of 4-diphosphocytidyl-2C-methyl-D-erythritol (CDP-me) from CTP and 2C-methyl-D-erythritol 4-phosphate (MEP). 4-diphosphocytidyl-2C-methyl-D-erythritol synthase IspD (MEP cytidylyltransferase) (MCT) Rv3582c, (MT3688, MTCY06G11.29c), len: 231 aa. ispD, 4-diphosphocytidyl-2C-methyl-D-erythritol synthase , equivalent to Q9CCW6|ML0321 putative 4-diphosphocytidyl-2C-methyl-D-erythritol synthase from Mycobacterium leprae (241 aa), FASTA scores: opt: 694, E(): 1.7e-35, (66.95% identity in 236 aa overlap). Also highly similar to others e.g. Q9L0Q8|ISPD_STRCO|SCD8A.06 from Streptomyces coelicolor (270 aa), FASTA scores: opt: 537, E(): 7.5e-26, (43.4% identity in 242 aa overlap); P74323|ISPD_SYNY3|SLR0951 from Synechocystis sp. strain PCC 6803 (230 aa), FASTA scores: opt: 410, E(): 3.8e-18, (36.15% identity in 224 aa overlap); Q9KGF8|ISPD_BACHD|BH0107 from Bacillus halodurans (228 aa) FASTA scores: opt: 367, E(): 1.6e-15, (34.65% identity in 228 aa overlap); Q08113|ISDF_RHOCA|ISPDF from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (379 aa)FASTA scores: opt: 359, E(): 7.8e-15, (34.1% identity in 223 aa overlap) (only similar with N-terminus of this bifunctional protein ISPD and ISPF); Q46893|ISPD_ECOLI|B2747 from Escherichia coli strain K12 (235 aa), FASTA scores: opt: 336, E(): 1.3e-13, (33.65% identity in 223 aa overlap); etc. Belongs to the ISPD family. P9WKG9 No intermediary metabolism and respiration 2XWN,3OKR,3Q7U,3Q80 P96864 P9WKG9 GO:0016114,GO:0050518 2.7.7.60 P9WKG9 NC_000962.3 Mycobrowser_v5 CDS 4034352 4035710 . + 0 Rv3593 lpqF Unknown Probable conserved lipoprotein LpqF Rv3593, (MTCY6F7.01c), len: 452 aa. Probable lpqF, conserved lipoprotein, equivalent to Q9CBI7|MPQF|ML1923 probale secreted protein from Mycobacterium leprae (454 aa), FASTA scores: opt: 2465, E(): 5.7e-144, (79.15% identity in 451 aa overlap). Also similar to Q9KJ91 hypothetical 47.1 KDA protein from Streptomyces clavuligerus (430 aa), FASTA scores: opt: 609, E(): 5.2e-30, (30.3% identity in 350 aa overlap); and some similarity with putative beta-lactamases e.g. Q9RYR7|DRA0241 beta lactamase-related protein from Deinococcus radiodurans (499 aa), FASTA scores: opt: 322, E(): 2.5e-12, (28.25% identity in 322 aa overlap). Equivalent to AAK48057 from Mycobacterium tuberculosis strain CDC1551 (438 aa) but longer 14 aa. Contains N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O06155 No cell wall and cell processes O06155 O06155 NC_000962.3 Mycobrowser_v5 CDS 4041423 4042940 . - 0 Rv3598c lysS Involved in translation [catalytic activity: ATP + L-lysine + tRNA(LYS) = AMP + pyrophosphate + L-lysyl-tRNA(LYS)]. Lysyl-tRNA synthetase 1 LysS (lysine--tRNA ligase 1) (LysRS 1) (lysine translase) Rv3598c, (MTCY07H7B.24), len: 505 aa. Probable lysS, lysyl-tRNA synthetase 1, equivalent to P46861|SYK_MYCLE|LYSS|ML0233 lysyl-tRNA synthetase from Mycobacterium leprae (507 aa), FASTA scores: opt: 2835, E(): 4.5e-172, (85.45% identity in 501 aa overlap); and similar with C-terminal part of Q9CC23|LYSX|ML1393 C-term lysyl-tRNA synthase from Mycobacterium leprae (1039 aa) FASTA scores: opt: 1257, E(): 7.6e-72, (44.55% identity in 505 aa overlap). Also similar to others e.g. P37477|SYK_BACSU|LYSS from Bacillus subtilis (499 aa) FASTA scores: opt: 1294, E(): 1.9e-74, (42.35% identity in 498 aa overlap); Q9RHV9|SYK_BACST|LYSS from Bacillus stearothermophilus (494 aa), FASTA scores: opt: 1258, E(): 3.5e-72, (41.15% identity in 498 aa overlap); Q9PEB6|SYK_XYLFA|LYSS|XF1112 from Xylella fastidiosa (506 aa), FASTA scores: opt: 1228, E(): 2.9e-70, (43.05% identity in 495 aa overlap); etc. Also similar to P94974|SYK2_MYCTU|LYSS2|LYSX|Rv1640c|MTCY06H11.04c lysyl-tRNA synthetase 2 from Mycobacterium tuberculosis (1172 aa), FASTA scores: opt: 1295, E(): 3.3e-74, (45.65% identity in 506 aa overlap). Contains PS00179 Aminoacyl-transfer RNA synthetases class-II signature 1. Belongs to class-II aminoacyl-tRNA synthetase family. P9WFU9 No information pathways P67607 P9WFU9 GO:0005737,GO:0004824,GO:0006430,GO:0000287,GO:0003676,GO:0005524 6.1.1.6 P9WFU9 NC_000962.3 Mycobrowser_v5 CDS 4044281 4045210 . - 0 Rv3602c panC Involved in pantothenate biosynthesis [catalytic activity: ATP + (R)-pantoate + beta-alanine = AMP + pyrophosphate + (R)-pantothenate]. Pantoate--beta-alanine ligase PanC (pantothenate synthetase) (pantoate activating enzyme) Rv3602c, (MTCY07H7B.20), len: 309 aa. panC, pantoate--beta-alanine ligase, equivalent to O69524|PANC_MYCLE|ML0230|MLCB2548.01c pantoate--beta-alanine ligase from Mycobacterium leprae (313 aa), FASTA scores: opt: 1541, E(): 3.4e-84, (82.15% identity in 297 aa overlap). Also similar to others e.g. O67891|PANC_AQUAE|AQ_2132 from Aquifex aeolicus (282 aa) FASTA scores: opt: 774, E(): 8.6e-39, (46.9% identity in 273 aa overlap); Q9HV69|PANC_PSEAE|PA4730 from Pseudomonas aeruginosa (283 aa), FASTA scores: opt: 770, E(): 1.5e-38, (51.45% identity in 276 aa overlap); Q9A6C8|CC2166 from Caulobacter crescentus (285 aa), FASTA scores: opt: 744, E(): 5.2e-37, (47.75% identity in 268 aa overlap); P31663|PANC_ECOLI|B0133 from Escherichia coli strain K12 (283 aa), FASTA scores: opt: 695, E(): 4.1e-34, (46.1% identity in 271 aa overlap); etc. Belongs to the pantothenate synthetase family. P9WIL5 No intermediary metabolism and respiration 1MOP,1N2B,1N2E,1N2G,1N2H,1N2I,1N2J,1N2O,2A7X,2A84,2A86,2A88,3COV,3COW,3COY,3COZ,3IMC,3IME,3IMG,3IOB,3IOC,3IOD,3IOE,3ISJ,3IUB,3IUE,3IVC,3IVG,3IVX,3LE8 P0A5R0 P9WIL5 GO:0005737,GO:0046872,GO:0004592,GO:0015940,GO:0005524 6.3.2.1 P9WIL5 NC_000962.3 Mycobrowser_v5 CDS 4048181 4048747 . - 0 Rv3606c folK Involved in dihydrofolate biosynthesis (at the first step) [catalytic activity: ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine = AMP + 2-amino-7,8-dihydro-4-hydroxy-6-(diphosphooxymethyl)pteridine]. 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase FolK (7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase) (HPPK) (6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase) (PPPK) (2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase) (7,8-dihydro-6-hydroxymethylpterin-diphosphokinase) (6-hydroxymethyl-7,8-dihydropterin diphosphokinase) Rv3606c, (MTCY07H7B.16), len: 188 aa. Probable folK, 2-amino-4-hydroxy-6-hydroxymethyldihydropterine pyrophosphokinase, equivalent to O69528|HPPK_MYCLE|folk|ML0226MLCB2548.05c 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase from Mycobacterium leprae (191 aa) FASTA scores: opt: 772, E(): 1.2e-44, (63.15% identity in 190 aa overlap). Also similar to many e.g. P71512|HPPK_METEX|folk|FOLA from Methylobacterium extorquens (158 aa), FASTA scores: opt: 292, E(): 1.4e-12, (36.85% identity in 171 aa overlap); O33726|HPPK_STRPY|folk|SPY1100 from Streptococcus pyogenes (166 aa), FASTA scores: opt: 234, E(): 1.1e-08, (34.3% identity in 175 aa overlap); Q9X8I1|SCE9.08 from Streptomyces coelicolor (203 aa), FASTA scores: opt: 232, E(): 1.7e-08, (43.25% identity in 185 aa overlap); P26281|HPPK_ECOLI|folk|B0142 from Escherichia coli strain K12 (158 aa), FASTA scores: opt: 198, E(): 2.6e-06, (32.85% identity in 143 aa overlap); etc. Belongs to the HppK family. P9WNC7 No intermediary metabolism and respiration P64143 P9WNC7 GO:0005524,GO:0046656,GO:0016301,GO:0003848 2.7.6.3 P9WNC7 NC_000962.3 Mycobrowser_v5 CDS 4048744 4049145 . - 0 Rv3607c folB Involved in folate biosynthesis. Catalyzes the conversion of 7,8-dihydroneopterin to 6- hydroxymethyl-7,8-dihydropterin [catalytic activity: 2-amino-4-hydroxy-6-(D-erythro-1,2,3-trihydroxypropyl)-7,8-dihydropteridine = 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine + glycolaldehyde]. Probable dihydroneopterin aldolase FolB (DHNA) Rv3607c, (MTCY07H7B.15), len: 133 aa. Probable folB, dihydroneopterin aldolase, equivalent to O69529|FOLB_MYCLE|ML0225|MLCB2548.06c probable dihydroneopterin aldolase from Mycobacterium leprae (132 aa), FASTA scores: opt: 673, E(): 5.1e-37, (74.8% identity in 131 aa overlap). Also similar to many e.g. Q9X8I0|FOLB_STRCO|SCE9.07 from Streptomyces coelicolor (119 aa), FASTA scores: opt: 334, E(): 4.5e-15, (46.15% identity in 117 aa overlap); P74342|FOLB_SYNY3|SLR1626 from Synechocystis sp. strain PCC 6803 (118 aa) FASTA scores: opt: 287, E(): 5e-12, (38.45% identity in 117 aa overlap); P28823|FOLB_BACSU|FOLA from Bacillus subtilis (120 aa), FASTA scores: opt: 283, E(): 9.2e-12, (39.0% identity in 118 aa overlap); etc. Belongs to the DHNA family. Note that previously known as folX. P9WNC5 No intermediary metabolism and respiration 1NBU,1Z9W P0A580 P9WNC5 GO:0046656,GO:0004150 4.1.2.25 P9WNC5 NC_000962.3 Mycobrowser_v5 CDS 4049138 4049980 . - 0 Rv3608c folP1 Involved in dihydrofolate biosynthesis (at the second step). Catalyzes the formation of the immediate precursor of FOLIC acid. It is implicated in resistance to sulfonamide [catalytic activity: 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate = pyrophosphate + dihydropteroate]. Dihydropteroate synthase 1 FolP (DHPS 1) (dihydropteroate pyrophosphorylase 1) (dihydropteroate diphosphorylase 1) Rv3608c, (MTCY07H7B.14), len: 280 aa. Probable folP1, dihydropteroate synthase 1, equivalent to O69530|FOLP (alias Q9S0T0|FOLP and Q9R2U9|FOLP) dihydroneopterin aldolase from Mycobacterium leprae (284 aa), FASTA scores: opt: 1418, E(): 7.2e-77, (76.75% identity in 284 aa overlap). Also highly similar to many e.g. Q9X8H8|SCE9.05 from Streptomyces coelicolor (288 aa), FASTA scores: opt: 953, E(): 2.4e-49, (56.0% identity in 266 aa overlap); Q9A3I0|CC3224 from Caulobacter crescentus (274 aa), FASTA scores: opt: 682, E(): 2.6e-33, (45.5% identity in 268 aa overlap); P73248|DHPS_SYNY3|FOLP|SLR2026 from Synechocystis sp. strain PCC 6803 (289 aa), FASTA scores: opt: 665, E(): 2.7e-32, (44.55% identity in 265 aa overlap); P26282|DHPS_ECOLI|FOLP|B3177 from Escherichia coli strain K12 (282 aa), FASTA scores: opt: 642, E(): 6.1e-31, (41.95% identity in 274 aa overlap); etc. Contains PS00792 Dihydropteroate synthase signature 1, PS00793 Dihydropteroate synthase signature 2. Similar to other species DHPS. P9WND1 No intermediary metabolism and respiration 1EYE P0A578 P9WND1 GO:0046656,GO:0046677,GO:0004156 2.5.1.15 P9WND1 NC_000962.3 Mycobrowser_v5 CDS 4054142 4054696 . - 0 Rv3614c espD Function unknown ESX-1 secretion-associated protein EspD Rv3614c, (MTCY07H7B.08), len: 184 aa. EspD, ESX-1 secretion-associated protein, equivalent to Q49730|ML0407|B1620_C3_264|MLCL383.03 hypothetical 24.2 KDA protein from Mycobacterium leprae (216 aa) FASTA scores: opt: 899, E(): 1.7e-51, (71.3% identity in 188 aa overlap); and similar to two hypothetical proteins from Mycobacterium leprae: Q9CDD6|ML0056 (169 aa), FASTA scores: opt: 285, E(): 1.2e-11, (38.35% identity in 172 aa overlap); and O33090|MLCB628.19c (338 aa), FASTA scores: opt: 289, E(): 1.2e-11, (38.95% identity in 172 aa overlap). Also highly similar to O69732|Rv3867|MTV027.02 hypothetical 19.9 KDA protein from Mycobacterium tuberculosis (183 aa), FASTA scores: opt: 563, E(): 1e-29, (54.9% identity in 173 aa overlap). Rv3614c and Rv3882c interact, by yeast two-hybrid analysis (See MacGurn et al., 2005). EspD|Rv3614c is still secreted by M. tuberculosis H37Rv and Erdman ESX-1 secretion system mutants, but at levels lower than in wild-type (See Chen et al., 2012). P9WJD5 No cell wall and cell processes P9WJD5 GO:0009405 NC_000962.3 Mycobrowser_v5 CDS 4065900 4067285 . - 0 Rv3627c Rv3627c Function unknown (possibly involved in cell wall biosynthesis). Conserved protein Rv3627c, (MTCY15C10.25), len: 461 aa. Conserved ala-rich protein which may have cleavable signal peptide at N-terminal end. Equivalent to O69539|MLCB2548.20c|ML0211 hypothetical 47.2 KDA protein from Mycobacterium leprae (461 aa), FASTA scores: opt: 2295, E(): 3.5e-116, (76.2% identity in 462 aa overlap); and C-terminal end shows similarity with O05758|MLCB5.28c hypothetical 24.1 KDA protein from Mycobacterium leprae (225 aa), FASTA scores: opt: 268, E(): 1.8e-07, (32.25% identity in 220 aa overlap). Also similar (or with similarity) to various proteins (notably penicillin binding proteins) e.g. Q9X8I8|SCE9.15c hypothetical 45.9 KDA protein from Streptomyces coelicolor (459 aa) FASTA scores: opt: 707, E(): 8.3e-31, (35.75% identity in 439 aa overlap); Q9Z541|SC9B2.18c putative carboxypeptidase from Streptomyces coelicolor (451 aa), FASTA scores: opt: 450, E(): 5.3e-17, (31.75% identity in 469 aa overlap); Q9JVV4|NMA0665 putative peptidase from Neisseria meningitidis (serogroup A) (or Q9JY10|NMB1797 from serogroup B) (469 aa), FASTA scores: opt: 269, E(): 3e-07, (26.15% identity in 463 aa overlap); O85665|PBP3 penicillin binding protein 3 from Neisseria gonorrhoeae (469 aa), FASTA scores: opt: 265, E(): 4.9e-07, (31.85% identity in 201 aa overlap); P45161|PBP4_HAEIN|DACB|HI1330 penicillin-binding protein 4 precursor/peptidase (479 aa) FASTA scores: opt: 230, E(): 3.8e-05, (27.9% identity in 394 aa overlap); P24228|PBP4_ECOLI|DACB|B3182 penicillin-binding protein 4 precursor from Escherichia coli strain K12 (477 aa), FASTA scores: opt: 166, E(): 0.1, (28.2% identity in 408 aa overlap); etc. Predicted to be an outer membrane protein (See Song et al., 2008). O06380 No conserved hypotheticals O06380 O06380 GO:0004185,GO:0006508 O06380 NC_000962.3 Mycobrowser_v5 CDS 4105459 4107084 . - 0 Rv3666c dppA Thought to be involved in active transport of dipeptide across the membrane (import). Probable periplasmic dipeptide-binding lipoprotein DppA Rv3666c, (MTV025.014c), len: 541 aa. Probable dppA, dipeptide-binding lipoprotein component of dipeptide transport system (see citation below), similar to many substrate-binding proteins e.g. Q9F353|SC9E12.02 putative peptide transport system secreted peptide-binding protein from Streptomyces coelicolor (544 aa), FASTA scores: opt: 1200, E(): 9e-67, (39.2% identity in 538 aa overlap); P24141|OPPA_BACSU oligopeptide-binding protein from Bacillus subtilis (545 aa), FASTA scores: opt: 523, E(): 7.9e-25, (26.15% identity in 516 aa overlap); P23843|OPPA_ECOLI periplasmic oligopeptide-binding protein from Escherichia coli (543 aa), FASTA scores: opt: 452, E(): 2e-20, (25.9% identity in 529 aa overlap); etc. Contains probable N-terminal signal sequence. I6X811 No cell wall and cell processes O69634 I6X811 GO:0005215,GO:0006810 I6X811 NC_000962.3 Mycobrowser_v5 CDS 4055197 4056375 . - 0 Rv3616c espA Function unknown ESX-1 secretion-associated protein A, EspA Rv3616c, (MTCY07H7B.06), len: 392 aa. EspA, ESX-1 secretion-associated protein A. Ala-, gly-rich protein, equivalent to Q49722|ML0405|B1620_C2_213|MLCL383.01 hypothetical 40.8 KDA protein from Mycobacterium leprae (394 aa) FASTA scores: opt: 1620, E(): 5.3e-75, (62.7% identity in 394 aa overlap). Also similar to P96213|Rv3864|MTCY01A6.04c hypothetical 42.1 KDA protein from Mycobacterium tuberculosis (402 aa), FASTA scores: opt: 389, E(): 1.1e-12, (31.75% identity in 400 aa overlap). P9WJE1 No cell wall and cell processes P9WJE1 GO:0009405,GO:0005576 NC_000962.3 Mycobrowser_v5 CDS 4058698 4059885 . + 0 Rv3618 Rv3618 Function unknown Possible monooxygenase Rv3618, (MTCY15C10.34c, MTCY07H7B.04c), len: 395 aa. Possible monooxygenase, similar to others (principally bacterial luciferases alpha chain) e.g. Q9JN87|MMYO putative alkanal monooxygenase from Streptomyces coelicolor (373 aa), FASTA scores: opt: 949, E(): 8.9e-54, (41.7% identity in 374 aa overlap); Q9EUT9|limb limonene monooxygenase from Rhodococcus erythropolis (387 aa), FASTA scores: opt: 856, E(): 9.1e-48, (42.0% identity in 388 aa overlap); AAK72698 LUXA-like protein from Bradyrhizobium japonicum (458 aa) FASTA scores: opt: 350, E(): 4.4e-15, (29.7% identity in 347 aa overlap); Q9K4C1|2SC6G5.34c putative alkanal monooxygenase (luciferase) from Streptomyces coelicolor (342 aa), FASTA scores: opt: 291, E(): 2.2e-11, (26.5% identity in 362 aa overlap); etc. Also similar to P95278|Rv1936|MTCY09F9.28c hypothetical 41.8 KDA protein from Mycobacterium tuberculosis (369 aa), FASTA scores: opt: 473, E(): 4.3e-23, (32.55% identity in 378 aa overlap). I6X7W8 No intermediary metabolism and respiration O07914 I6X7W8 GO:0055114,GO:0004497 1.-.-.- I6X7W8 NC_000962.3 Mycobrowser_v5 CDS 4063254 4063904 . - 0 Rv3624c hpt Involved in purine salvage [catalytic activity: imp + pyrophosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate (guanine can replace hypoxanthine to produce GMP)]. Hypoxanthine-guanine phosphoribosyltransferase Hpt (HGPRT) (HGPRTase) (hypoxanthine phosphoribosyltransferase) (imp pyrophosphorylase) (imp diphosphorylase) (transphosphoribosyltransferase) (guanine phosphoribosyltransferase) Rv3624c, (MTCY15C10.28), len: 216 aa. Hpt (alternate gene name: hprT), hypoxanthine-guanine phosphoribosyltransferase (but seems to have a 35 aa extension at N-terminus), equivalent to other mycobacterial hypoxanthine-guanine phosphoribosyltransferases e.g. P96794 from Mycobacterium avium (203 aa), FASTA scores: opt: 1136, E(): 1.2e-65, (88.5% identity in 200 aa overlap); and O69537|HPT|ML0214 from Mycobacterium leprae (213 aa), FASTA scores: opt: 1115, E(): 2.8e-64, (81.6% identity in 212 aa overlap). Also similar to others e.g. Q9X8I5|SCE9.12c from Streptomyces coelicolor (187 aa), FASTA scores: opt: 724, E(): 2.4e-39, (60.55% identity in 180 aa overlap); P37472|HPRT_BACSU|HPT from Bacillus subtilis (180 aa) FASTA scores: opt: 574, E(): 9.1e-30, (48.6% identity in 181 aa overlap); etc. Equivalent to AAK48087 from Mycobacterium tuberculosis strain CDC1551 (202 aa) but longer 14 aa. Contains PS00103 Purine/pyrimidine phosphoribosyltransferases signature. Belongs to the purine/pyrimidine phosphoribosyltransferase family. P9WHQ9 No intermediary metabolism and respiration P0A5T0 P9WHQ9 GO:0004422,GO:0046872,GO:0006166,GO:0005737 2.4.2.8 P9WHQ9 NC_000962.3 Mycobrowser_v5 CDS 4102032 4103678 . - 0 Rv3663c dppD Involved in active transport of dipeptide across the membrane (import). Responsible for energy coupling to the transport system. Probable dipeptide-transport ATP-binding protein ABC transporter DppD Rv3663c, (MTV025.011c), len: 548 aa. Probable dppD, dipeptide-transport ATP-binding protein ABC-transporter (see citation below), similar to many ATP-binding proteins e.g. AAK65441|SMA1434 probable ABC transporter ATP-binding protein from Rhizobium meliloti (Sinorhizobium meliloti) plasmid pSymA (550 aa), FASTA scores: opt: 1528, E(): 1e-78, (46.25% identity in 545 aa overlap); O50270|MOAD MOAD protein from Agrobacterium radiobacter (588 aa), FASTA scores: opt: 1354, E(): 6.7e-69, (42.9% identity in 541 aa overlap); Q9KM01|VCA0588 putative peptide ABC transporter ATP-binding protein from Vibrio cholerae (530 aa), FASTA scores: opt: 951, E(): 3.1e-46, (44.0% identity in 534 aa overlap); BAB49448|MLR2279 ATP-binding protein of peptide ABC transporter from Rhizobium loti (Mesorhizobium loti) (604 aa), FASTA scores: opt: 949, E(): 4.4e-46, (41.55% identity in 544 aa overlap); etc. Contains 2 PS00211 ABC transporters family signature, and 2 PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters). I6Y482 No cell wall and cell processes O69631 I6Y482 GO:0016887,GO:0015833,GO:0005524 I6Y482 NC_000962.3 Mycobrowser_v5 CDS 4067423 4067911 . + 0 Rv3628 ppa Involved in the function of cellular bioenergetics [catalytic activity: pyrophosphate + H(2)O = 2 orthophosphate]. Inorganic pyrophosphatase Ppa (pyrophosphate phospho-hydrolase) (PPASE) (inorganic diphosphatase) (diphosphate phospho-hydrolase) Rv3628, (MTCY15C10.24), len: 162 aa. Ppa, inorganic pyrophosphatase (see Triccas & Gicquel 2001), identical to O69540|IPYR_MYCLEPPA|ML0210|MLCB2548.21 inorganic pyrophosphatase from Mycobacterium leprae (162 aa) FASTA scores: opt: 1018, E(): 1.3e-59, (89.5% identity in 162 aa overlap). Also highly similar to many bacterial pyrophosphatases e.g. Q9X8I9|IPYR_STRCO|PPA|SCE9.16 from Streptomyces coelicolor (163 aa), FASTA scores: opt: 773, E(): 1.3e-43, (67.5% identity in 163 aa overlap); O05545|IPYR_GLUOX|PPA from Gluconobacter oxydans (Gluconobacter suboxydans) (176 aa), FASTA scores: opt: 553, E(): 3.2e-29, (53.8% identity in 145 aa overlap); P77992|IPYR_THELI|PPA from Thermococcus litoralis (176 aa) FASTA scores: opt: 537, E(): 3.5e-28, (49.35% identity in 152 aa overlap); P50308|IPYR_SULAC|PPA from Sulfolobus acidocaldarius (173 aa), FASTA scores: opt: 518, E(): 6e-27, (45.3% identity in 159 aa overlap); etc. Belongs to the PPASE family. Cofactor: requires the presence of divalent metal cation. Magnesium confers the highest activity. Binds 4 divalent cations per subunit (by similarity). P9WI55 No intermediary metabolism and respiration 1SXV,1WCF,2UXS P65746 P9WI55 GO:0004427,GO:0000287,GO:0006796,GO:0005737 3.6.1.1 P9WI55 NC_000962.3 Mycobrowser_v5 CDS 4069175 4070470 . + 0 Rv3630 Rv3630 Unknown Probable conserved integral membrane protein Rv3630, (MTCY15C10.22c), len: 431 aa. Probable conserved integral membrane, highly similar to P71789|YF10_MYCTU|Rv1510|MTCY277.32 hypothetical 44.3 KDA protein from Mycobacterium tuberculosis (432 aa) FASTA scores: opt: 1940, E(): 2.3e-103, (70.75% identity in 424 aa overlap). Note that N-terminal end is highly similar to AAK45825|MT1558 hypothetical 18.1 KDA protein from Mycobacterium tuberculosis strain CDC1551 (172 aa) FASTA scores: opt: 649, E(): 4.2e-30, (61.65% identity in 167 aa overlap); and C-terminal end is highly similar to AAK45826|MT1560 hypothetical 25.8 KDA protein from Mycobacterium tuberculosis strain CDC1551 (256 aa), FASTA scores: opt: 1269, E(): 2.6e-65, (76.7% identity in 253 aa overlap). Contains PS00639 Eukaryotic thiol (cysteine) proteases histidine active site, so could be a protease. P9WKX9 No cell wall and cell processes P9WKX9 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 4070514 4071239 . + 0 Rv3631 Rv3631 Function unknown; probably involved in cellular metabolism. Possible transferase (possibly glycosyltransferase) Rv3631, (MTCY15C10.21c), len: 241 aa. Possible transferase, more specifically a glycosyltransferase , equivalent to O69542|MLCB2548.24c|ML0207 putative transferase (putative glycosyltransferase) from Mycobacterium leprae (239 aa) FASTA scores: opt: 1303, E(): 2.8e-72, (81.2% identity in 239 aa overlap). Also similar to many dolichyl-phosphate mannose synthases and hypothetical proteins e.g. O59263|PH1585 hypothetical 34.6 KDA protein from Pyrococcus horikoshii (313 aa), FASTA scores: opt: 472, E(): 1.2e-21, (36.65% identity in 232 aa overlap); Q9V152|PAB1971 dolichyl-phosphate mannose synthase from Pyrococcus abyssi (287 aa), FASTA scores: opt: 467, E(): 2.3e-21, (35.85% identity in 223 aa overlap); Q58619|YC22_METJA|MJ1222 hypothetical protein from Methanococcus jannaschii (243 aa), FASTA scores: opt: 400, E(): 2.4e-17, (33.35% identity in 228 aa overlap); O26474|MTH374 dolichyl-phosphate mannose synthase related protein from Methanobacterium thermoautotrophicum (291 aa) FASTA scores: opt: 354, E(): 1.7e-14, (33.5% identity in 218 aa overlap); O26239|MTH136 dolichyl-phosphate mannose synthase from Methanobacterium thermoautotrophicum (220 aa), FASTA scores: opt: 345, E(): 4.8e-14, (33.5% identity in 221 aa overlap); etc. O06376 No intermediary metabolism and respiration O06376 O06376 GO:0016740 2.-.-.- O06376 NC_000962.3 Mycobrowser_v5 CDS 4076484 4076984 . + 0 Rv3637 Rv3637 Possibly required for the transposition of an insertion element. Possible transposase Rv3637, (MTCY15C10.15c), len: 166 aa. Possible transposase. C-terminal end highly similar to Q9RLQ9|ISTA putative transposase a (fragment) from Mycobacterium bovis (102 aa), FASTA scores: opt: 397, E(): 1.4e-19, (58.8% identity in 102 aa overlap). Weakly similar to others e.g. Q9KJ02 putative transposase (fragment) from Polyangium cellulosum (329 aa), FASTA scores: opt: 191, E(): 1.6e-05, (32.1% identity in 134 aa overlap); Q9LCU2|ISTA cointegrase from Pseudomonas aeruginosa (382 aa) FASTA scores: opt: 144, E(): 0.024, (26.8% identity in 123 aa overlap); P15025|ISTA_PSEAE transposase for insertion sequence element IS21 from Pseudomonas aeruginosa (390 aa), FASTA scores: opt: 144, E(): 0.025, (26.85% identity in 123 aa overlap); etc. Also highly similar to C-terminal end of P96288|Rv2943|MTCY24G1.06c hypothetical 45.8 KDA protein from Mycobacterium tuberculosis (413 aa) FASTA scores: opt: 722, E(): 1.5e-40, (63.7% identity in 168 aa overlap). O06370 No insertion seqs and phages O06370 O06370 NC_000962.3 Mycobrowser_v5 CDS 4076984 4077730 . + 0 Rv3638 Rv3638 Possibly required for the transposition of an insertion element. Possible transposase Rv3638, (MTCY15C10.14c), len: 248 aa. Possible transposase, highly similar to Q9RLQ8|ISTB ISTB protein from Mycobacterium bovis (266 aa), FASTA scores: opt: 784, E(): 4e-46, (78.0% identity in 259 aa overlap); and similar to others e.g. P15026|ISTB_PSEAE insertion sequence IS21 putative ATP-binding protein from Pseudomonas aeruginosa (265 aa), FASTA scores: opt: 420, E(): 2.2e-21, (38.8% identity in 255 aa overlap); Q45619|ISTB_BACST insertion sequence IS5376 putative ATP-binding protein from Bacillus stearothermophilus (251 aa), FASTA scores: opt: 402, E(): 3.6e-20, (34.5% identity in 232 aa overlap); P15026|ISTB_ECOLI ISTB protein from Escherichia coli (265 aa), FASTA scores: opt: 419, E(): 8e-23, (38.8% identity in 255 aa overlap); etc. C-terminus highly similar to C-terminus of P96287|Rv2944|MTCY24G1.05 hypothetical 25.5 KDA protein from Mycobacterium tuberculosis strain H37Rv (alias AAK47343|MT3016 IS1533, ORFB from Mycobacterium tuberculosis strain CDC1551) (238 aa), FASTA scores: opt: 784, E(): 3.6e-46, (87.4% identity in 135 aa overlap). I6XHU7 No insertion seqs and phages O06369 I6XHU7 GO:0005524 I6XHU7 NC_000962.3 Mycobrowser_v5 CDS 4081516 4082721 . - 0 Rv3644c Rv3644c DNA polymerase is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. Possible DNA polymerase Rv3644c, (MTCY15C10.08), len: 401 aa. Possible DNA polymerase, equivalent to O69546|MLCB2548.29c|ML0202 hypothetical 42.7 KDA protein from Mycobacterium leprae (405 aa), FASTA scores: opt: 2180, E(): 6.1e-116, (84.4% identity in 404 aa overlap). Similar (in totality or in first 200 aa) to DNA polymerases III, delta' or gamma subunit, e.g. Q9X906|SCH5.03c putative DNA polymerase from Streptomyces coelicolor (401 aa), FASTA scores: opt: 1022, E(): 1.5e-50, (47.05% identity in 404 aa overlap); Q9RRS5|DR2410 DNA polymerase III, tau/gamma subunit from Deinococcus radiodurans (615 aa), FASTA scores: opt: 370, E(): 1.3e-13, (29.95% identity in 394 aa overlap); P28631|HOLB_ECOLI|B1099 DNA polymerase III, delta' subunit from Escherichia coli strain K12 (334 aa), FASTA scores: opt: 345, E(): 2.2e-12, (33.45% identity in 239 aa overlap); Q9JTS1|DNAZX|NMA1656 DNA polymerase III tau and gamma chains from Neisseria meningitidis (serogroup A) (709 aa), FASTA scores: opt: 346, E(): 3.3e-12, (28.55% identity in 364 aa overlap); etc. O06363 No information pathways O06363 O06363 GO:0005524,GO:0006260,GO:0003887,GO:0017111,GO:0008408 2.7.7.- O06363 NC_000962.3 Mycobrowser_v5 CDS 4088328 4088531 . - 0 Rv3648c cspA Possibly involved in cold acclimation processes (the production of the protein is supposedly predominantly induced at low temperatures). Probable cold shock protein A CspA Rv3648c, (MTCY15C10.04), len: 67 aa. Probable cspA, cold shock protein A, identical to O69550|CSPB|CSPA|ML0198 small cold-shock protein from Mycobacterium leprae (67 aa) FASTA scores: opt: 451, E(): 3.7e-27, (97.0% identity in 67 aa overlap). Also highly similar to many e.g. Q9KGW0|CSPA from Mycobacterium smegmatis (67 aa) FASTA scores: opt: 439, E(): 2.9e-26, (92.55% identity in 67 aa overlap); P54584|CSP_ARTGO from Arthrobacter globiformis (67 aa), FASTA scores: opt: 335, E(): 1.5e-18, (73.45% identity in 64 aa overlap); O30875|CSPA_MICLU from Micrococcus luteus (Micrococcus lysodeikticus); Q9Z5R4|CSPA_BORPE from Bordetella pertussis (67 aa) FASTA scores: opt: 294, E(): 1.7e-15, (59.7% identity in 67 aa overlap); etc. Contains 'cold-shock' DNA-binding domain signature (PS00352) at N-terminal end. Belongs to the cold-shock domain (CSD) family. P9WP75 No virulence, detoxification, adaptation P63848 P9WP75 GO:0005737,GO:0006355,GO:0006350,GO:0003677 P9WP75 NC_000962.3 Mycobrowser_v5 CDS 4088781 4091096 . + 0 Rv3649 Rv3649 Possibly has helicase activity. Probable helicase Rv3649, (MTCY15C10.03c), len: 771 aa. Probable helicase, similar to many (known or hypothetical) ATP-dependent helicases e.g. Q9X915|SCH5.13 putative helicase from Streptomyces coelicolor (815 aa) FASTA scores: opt: 2550, E(): 9.6e-139, (52.45% identity in 774 aa overlap); Q05549|YDR291W|D9819.1 protein similar to several DNA helicases from Saccharomyces cerevisiae (Baker's yeast) (1077 aa), FASTA scores: opt: 1161, E(): 5.9e-59, (31.05% identity in 780 aa overlap); P50830|YPRA_BACSU hypothetical helicase from Bacillus subtilis (749 aa), FASTA scores: opt: 1154, E(): 1.1e-58, (34.05% identity in 734 aa overlap); Q9KC10|BH1764 ATP-dependent RNA helicase from Bacillus halodurans (764 aa), FASTA scores: opt: 1122, E(): 8e-57, (32.3% identity in 759 aa overlap); etc. Seems similar to dead/DEAH box helicase family, and to helicase C-terminal domain. O06359 No information pathways O06359 O06359 GO:0008026,GO:0003676,GO:0005524 3.6.-.- O06359 NC_000962.3 Mycobrowser_v5 CDS 4096936 4097994 . - 0 Rv3659c Rv3659c Function unknown Conserved hypothetical protein Rv3659c, (MTV025.007c), len: 352 aa. Conserved hypothetical protein, highly similar, but always shorter (various lengths) at N-terminus, to Q9X921|SCH5.19c putative secretory protein from Streptomyces coelicolor (523 aa), FASTA scores: opt: 1287, E(): 5.3e-66, (59.85% identity in 351 aa overlap); Q9HW98|PA4302 probable type II secretion system protein from Pseudomonas aeruginosa (421 aa), FASTA scores: opt: 776, E(): 5.4e-37, (42.8% identity in 320 aa overlap); AAK65510|CPAF2 probable CPAF2 PILUS assembly protein from Rhizobium meliloti (Sinorhizobium meliloti) plasmid pSymA (497 aa) FASTA scores: opt: 769, E(): 1.5e-36, (40.45% identity in 309 aa overlap); Q9KY93|SCK15.11 putative secretory protein from Streptomyces coelicolor (445 aa), FASTA scores: opt: 751, E(): 1.5e-35, (38.15% identity in 333 aa overlap); etc. Contains PS00017 ATP/GTP binding site motif A (P-loop). Note that previously known as trbB. P9WMT3 No conserved hypotheticals Q7D547 P9WMT3 GO:0000746,GO:0005737,GO:0006810,GO:0005524 P9WMT3 NC_000962.3 Mycobrowser_v5 CDS 4099647 4100510 . + 0 Rv3661 Rv3661 Possibly plays a regulatory role in celular differentiation. Conserved hypothetical protein Rv3661, (MTV025.009), len: 287 aa. Conserved hypothetical protein, highly similar to O33611|IMD_STRCN from Streptomyces cyaneus (Streptomyces curacoi) protein involved in inhibition of morphological differentiation in Streptomyces azureus (belongs to the SerB family) (277 aa) FASTA scores: opt: 1073, E(): 3.5e-61, (61.45% identity in 262 aa overlap); and Q9X923|SCH5.21 putative morphological differentiation-associated protein from Streptomyces coelicolor (268 aa), FASTA scores: opt: 1057, E(): 3.6e-60, (61.45% identity in 262 aa overlap). Also similar to various bacterial proteins (principally serB-related proteins) e.g. Q49823|ML2424 hypothetical SERB protein from Mycobacterium leprae (300 aa), FASTA scores: opt: 452, E(): 1.4e-21, (35.8% identity in 257 aa overlap); Q9WX12|SCE68.20 hypothetical 32.0 KDA protein from Streptomyces coelicolor (298 aa), FASTA scores: opt: 415, E(): 3.1e-19, (33.55% identity in 280 aa overlap); Q9RIT2|SERB phosphoserine phosphatase (fragment) from Streptomyces coelicolor (266 aa), FASTA scores: opt: 405, E(): 1.2e-18, (34.1% identity in 261 aa overlap); etc. Also similar to Q11169|Y505_MYCTU|Rv0505c|MTCY20G9.32c hypothetical 39.5 KDA protein from Mycobacterium tuberculosis (373 aa), FASTA scores: opt: 454, E(): 1.2e-21, (35.15% identity in 276 aa overlap). Belongs to the SerB family. P9WGJ1 No virulence, detoxification, adaptation P9WGJ1 GO:0016791,GO:0008152 P9WGJ1 NC_000962.3 Mycobrowser_v5 CDS 4107792 4109747 . + 0 Rv3667 acs Activates acetate to acetyl-coenzyme A [catalytic activity: ATP + acetate + CoA = AMP + pyrophosphate + acetyl-CoA]. Acetyl-coenzyme A synthetase Acs (acetate--CoA ligase) (acetyl-CoA synthetase) (acetyl-CoA synthase) (acyl-activating enzyme) (acetate thiokinase) (acetyl-activating enzyme) (acetate--coenzyme A ligase) (acetyl-coenzyme A synthase) Rv3667, (MTV025.015), len: 651 aa. Probable acs, acetyl-coenzyme-a synthetase, similar to many e.g. Q9X928|SCH5.26 from Streptomyces coelicolor (651 aa) FASTA scores: opt: 2850, E(): 1.9e-164, (66.05% identity in 639 aa overlap); Q55404|ACSA_SYNY3|ACS|SLL0542 from Synechocystis sp. strain PCC 6803 (653 aa), FASTA scores: opt: 2342, E(): 8.8e-134, (55.15% identity in 649 aa overlap); P31638|ACSA_ALCEU|ACOE from Alcaligenes eutrophus (Ralstonia eutropha) (660 aa), FASTA scores: opt: 2181, E(): 4.6e-124, (52.05% identity in 665 aa overlap); P27550|ACSA_ECOLI|ACS|B4069 from Escherichia coli strain K12 (652 aa), FASTA scores: opt: 1625, E(): 0, (48.3% identity in 646 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family. P9WQD1 No lipid metabolism O69635 P9WQD1 GO:0005524,GO:0003987,GO:0008152,GO:0016208 6.2.1.1 P9WQD1 NC_000962.3 Mycobrowser_v5 CDS 4111346 4112329 . + 0 Rv3670 ephE Thought to be involved in detoxification reactions following oxidative damage to lipids. Biotransformation enzyme that catalyzes the hydrolysis of epoxides: aromatic hydrocarbons catabolism [catalytic activity: an epoxide + H(2)O = a glycol]. Possible epoxide hydrolase EphE (epoxide hydratase) (arene-oxide hydratase) Rv3670, (MTV025.018), len: 327 aa. Possible ephE, epoxide hydrolase (see citation below), equivalent to Q9CB96|ML2297 putative hydrolase from Mycobacterium leprae (324 aa), FASTA scores: opt: 1799, E(): 7.2e-105, (80.55% identity in 324 aa overlap). Also similar to many hydrolases (epoxide hydrolases) and hypothetical proteins e.g. Q9X931|SCH5.29 putative hydrolase from Streptomyces coelicolor (324 aa), FASTA scores: opt: 687, E(): 1.4e-35, (40.65% identity in 327 aa overlap); Q9RRE3|DR2549 epoxide hydrolase-related protein from Deinococcus radiodurans (278 aa), FASTA scores: opt: 321, E(): 8.2e-13, (32.15% identity in 311 aa overlap); Q9K3Q1|2SCG4.13 putative hydrolase from Streptomyces coelicolor (292 aa), FASTA scores: opt: 295, E(): 3.5e-11, (30.18% identity in 275 aa overlap); Q9S7P1 epoxide hydrolase from Oryza sativa (Rice) (322 aa), FASTA scores: opt: 289, E(): 9.1e-11, (28.7% identity in 338 aa overlap); O23227|C7A10.830|AT4G36530 epoxide hydrolase from Arabidopsis thaliana (Mouse-ear cress) (378 aa) FASTA scores: opt: 287, E(): 1.4e-10, (26.1% identity in 272 aa overlap); Q21147|K02F3.6 epoxide hydrolase from Caenorhabditis elegans (386 aa), FASTA scores: opt: 283, E(): 2.5e-10, (33.35% identity in 156 aa overlap); etc. Also similar to P95276|EPHB|Rv1938|MTCY09F9.26c from Mycobacterium tuberculosis (356 aa), FASTA scores: opt: 296, E(): 3.6e-11, (29.7% identity in 340 aa overlap). Contains PS00213 Lipocalin signature. Similar to alpha/beta hydrolase fold. I6YCQ4 No virulence, detoxification, adaptation O69638 I6YCQ4 GO:0016787 3.3.2.3 I6YCQ4 NC_000962.3 Mycobrowser_v5 CDS 4112322 4113515 . - 0 Rv3671c Rv3671c Function unknown; hydrolyses of peptides and/or proteins (possibly cleaved preferentially after serine residue). Membrane-associated serine protease Rv3671c, (MTV025.019c), len: 397 aa. Serine protease membrane protein, equivalent to Q9CB95|ML2298 putative membrane-associated serine protease from Mycobacterium leprae (401 aa), FASTA scores: opt: 2061, E(): 2.3e-108, (80.9% identity in 398 aa overlap). Also similar to many serine proteases, but generally with extended N-terminus, e.g. Q9X932|SCH5.30c putative serine protease (fragment) from Streptomyces coelicolor (385 aa), FASTA scores: opt: 835, E(): 1.2e-39, (39.9% identity in 386 aa overlap); Q9Z6T0|DEGP_CHLPN|HTRA|CPN0979|CP0877 probable serine protease do-like precursor from Chlamydia pneumoniae (Chlamydophila pneumoniae) (488 aa), FASTA scores: opt: 285, E(): 1e-08, (29.05% identity in 296 aa overlap); P73354|HTRA|SLR1204 serine protease from Synechocystis sp. strain PCC 6803 (452 aa), FASTA scores: opt: 284, E(): 1.1e-08, (29.55% identity in 308 aa overlap); Q9RWC4|DR0745 periplasmic serine protease, HTRA/DEGQ/DEGS family from Deinococcus radiodurans (366 aa), FASTA scores: opt: 271, E(): 4.9e-08, (35.45% identity in 206 aa overlap); etc. Also similar, but longer 114 aa at the N-terminus, to Q9S2P8|SC5F7.13 putative peptidase from Streptomyces coelicolor (282 aa), FASTA scores: opt: 594, E(): 3.1e-26, (38.95% identity in 285 aa overlap). And similar, but longer 146 aa at the N-terminus, to O07175|PEPA|Rv0125|MTCI418B.07 from Mycobacterium tuberculosis (355 aa), FASTA scores: opt: 295, E(): 2.2e-09, (29.55% identity in 254 aa overlap); and Q9CCY9|ML2659 probable secreted serine protease from Mycobacterium leprae FASTA scores: opt: 286, E(): 6.9e-09, (30.6% identity in 255 aa overlap). Contains PS00135 Serine proteases, trypsin family, serine active site. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P9WHR9 No intermediary metabolism and respiration 3K6Y,3K6Z,3LT3 O69639 P9WHR9 GO:0006508,GO:0004252,GO:0009403,GO:0016020 3.4.21.- P9WHR9 NC_000962.3 Mycobrowser_v5 CDS 4118776 4119798 . + 0 Rv3679 Rv3679 Anion-transporting ATPase; supposedly catalyzes the extrusion of undetermined anions [catalytic activity: ATP + H(2)O + undetermined anion(in) = ADP + phosphate + undetermined anion(out)]. Probable anion transporter ATPase Rv3679, (MTV025.027), len: 340 aa. Probable anion transporting ATPase, equivalent to Q9CB88|ML2305 probable anion transporter protein from Mycobacterium leprae (341 aa), FASTA scores: opt: 1810, E(): 2.1e-98, (84.15% identity in 341 aa overlap). Also highly similar to Q9XA36|SCH17.11 putative ion-transporting ATPase from Streptomyces coelicolor (325 aa), FASTA scores: opt: 989, E(): 1.4e-50, (52.15% identity in 328 aa overlap); and similar to many anion transporting ATPases (principally arsenite transporters) e.g. O50593|ARSA_ACIMU arsenical pump-driving ATPase (arsenite-translocating ATPase) from Acidiphilium multivorum (583 aa), FASTA scores: opt: 225, E(): 8.1e-06, (25.1% identity in 319 aa overlap); AAG43231|ARSA arsenite activated ATPase from Salmonella typhimurium plasmid R46 FASTA scores: opt: 211, E(): 5.3e-05, (26.95% identity in 267 aa overlap); P52145|ARA2_ECOLI|ARSA arsenical pump-driving ATPase from Escherichia coli plasmid IncN R46 (583 aa), FASTA scores: opt: 211, E(): 5.3e-05, (26.95% identity in 267 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Some similarity to the ARSA ATPase family. P9WKX5 No cell wall and cell processes P9WKX5 GO:0005524 P9WKX5 NC_000962.3 Mycobrowser_v5 CDS 4124417 4125376 . + 0 Rv3683 Rv3683 Function unknown Conserved protein Rv3683, (MTV025.031), len: 319 aa. Conserved protein, equivalent to Q9CB84|ML2309 hypothetical protein from Mycobacterium leprae (330 aa) FASTA scores: opt: 1791, E(): 9e-107, (85.45% identity in 296 aa overlap). Also similar to Q9X935|SCH66.03 conserved hypothetical protein from Streptomyces coelicolor (309 aa) FASTA scores: opt: 610, E(): 1.4e-31, (51.45% identity in 307 aa overlap); and Q9RRY7|YN45_DEIRA|DR2345 hypothetical protein from Deinococcus radiodurans (305 aa) FASTA scores: opt: 243, E(): 3.2e-08, (31.1% identity in 315 aa overlap) and some similarity to other hypothetical bacterial proteins e.g. Q9CF81|YQED from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (278 aa) FASTA scores: opt: 200, E(): 1.6e-05, (26.85% identity in 287 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). I6X827 No conserved hypotheticals O69651 I6X827 GO:0016787 I6X827 NC_000962.3 Mycobrowser_v5 CDS 4129323 4129691 . - 0 Rv3687c rsfB Regulates negatively Rv3287c|RSBW|USFX. Possibly regulated by phosphorylation. Anti-anti-sigma factor RsfB (anti-sigma factor antagonist) (regulator of sigma F B) Rv3687c, (MTV025.035c), len: 122 aa. RsfB, anti-anti-sigma factor (see citation below), showing some similarity to sporulation proteins and sigma-factor genes e.g. Q9WVX8|RSBV_STRCO|bldg|SCH5.12c anti-sigma B factor antagonist from Streptomyces coelicolor (113 aa) FASTA scores: opt: 163, E(): 0.0007, (31.15% identity in 106 aa overlap); Q9F3A2|SC5F1.27c putative anti-sigma factor antagonist from Streptomyces coelicolor (114 aa) FASTA scores: opt: 159, E(): 0.0013, (29.8% identity in 104 aa overlap); P73609|SLR1859 hypothetical 12.0 KDA protein from Synechocystis sp. strain PCC 6803 (108 aa) FASTA scores: opt: 152, E(): 0.0034, (32.2% identity in 90 aa overlap); L47358|BACSPOI_1 spoIIA a from Paenibacillus polymyxa (117 aa), FASTA scores: opt: 107, E(): 0.23, (24.8% identity in 113 aa overlap); SQSIGB_4 rsbU, rsbV, rsbW & sigB genes from Steptomyces aureus (108 aa) (28.3% identity in 60 aa overlap); etc. Also similar to hypothetical proteins from Mycobacterium tuberculosis e.g. MTCY180_14 and MTCY441 _8. P9WGE1 No information pathways O69655 P9WGE1 GO:0006355,GO:0045152 P9WGE1 NC_000962.3 Mycobrowser_v5 CDS 4137206 4138138 . + 0 Rv3695 Rv3695 Unknown Possible conserved membrane protein Rv3695, (MTV025.043), len: 310 aa. Possible conserved membrane protein, equivalent, but longer 88 aa, to Q9CB83|ML2312 possible membrane protein from Mycobacterium leprae (196 aa), FASTA scores: opt: 898, E(): 5.2e-36, (71.05% identity in 190 aa overlap). Also highly similar to Q9KZM3|SCE34.02 putative integral membrane protein from Streptomyces coelicolor (318 aa), FASTA scores: opt: 740,E(): 2.4e-28, (43.25% identity in 319 aa overlap); and similar to P72718|SLR0254 hypothetical 30.4 KDA protein from Synechocystis sp. strain PCC 6803 (266 aa), FASTA scores: opt: 287, E(): 6.1e-07, (29.6% identity in 260 aa overlap); Q9HW83|PA4318 hypothetical protein from Pseudomonas aeruginosa (265 aa), FASTA scores: opt: 250, E(): 3.5e-05, (32.0% identity in 203 aa overlap); Q9KEW5|BH0734 hypothetical protein from Bacillus halodurans (266 aa), FASTA scores: opt: 168, E(): 0.0047, (25.95% identity in 231 aa overlap); etc. C-terminal end shows some similarity to proline-rich proteins e.g. Q62106 proline-rich salivary protein (fragment) from Mus musculus (Mouse) (188 aa) (36.1% identity in 97 aa overlap). Equivalent to AAK48164 from Mycobacterium tuberculosis strain CDC1551 (269 aa) but longer 41 aa. O69663 No cell wall and cell processes O69663 O69663 O69663 NC_000962.3 Mycobrowser_v5 CDS 4138202 4139755 . - 0 Rv3696c glpK Acts in rate-limiting step in glycerol utilization. Key enzyme in the regulation of glycerol uptake and metabolism [catalytic activity: ATP + glycerol = ADP + glycerol 3-phosphate]. Probable glycerol kinase GlpK (ATP:glycerol 3-phosphotransferase) (glycerokinase) (GK) Rv3696c, (MTV025.044c), len: 517 aa. Probable glpK, glycerol kinase, equivalent to Q9CB81|GLPK_MYCLE|ML2314 glycerol kinase from Mycobacterium leprae (508 aa), FASTA scores: opt: 3120, E(): 4.7e-189, (91.35% identity in 508 aa overlap). Also highly similar to others e.g. Q9RJM2|GLPK from Streptomyces coelicolor (507 aa), FASTA scores: opt: 2606, E(): 1.1e-156, (75.35% identity in 503 aa overlap); Q9ADA7|GLPK from Streptomyces coelicolor (512 aa) FASTA scores: opt: 2002, E(): 1.3e-118, (59.05% identity in 503 aa overlap); Q9X1E4|GLK2_THEMA|TM1430 from Thermotoga maritima (496 aa), FASTA scores: opt: 1838, E(): 2.7e-108, (54.8% identity in 498 aa overlap); P08859|GLPK_ECOLI|B3926 from Escherichia coli strain K12 (501 aa), FASTA scores: opt: 1740, E(): 4.1e-102, (52.3% identity in 499 aa overlap); etc. Contains PS00933 FGGY family of carbohydrate kinases signature 1, PS00070 Aldehyde dehydrogenases cysteine active site, PS00445 FGGY family of carbohydrate kinases signature 2. Belongs to the fucokinase / gluconokinase / glycerokinase / xylulokinase family. P9WPK1 No intermediary metabolism and respiration O69664 P9WPK1 GO:0004370,GO:0006072,GO:0005524 2.7.1.30 P9WPK1 NC_000962.3 Mycobrowser_v5 CDS 4142748 4143920 . - 0 Rv3700c Rv3700c Function unknown; probably involved in cellular metabolism. Conserved hypothetical protein Rv3700c, (MTV025.048c), len: 390 aa. Conserved hypothetical protein; could be a transferase or a lyase. Indeed, similar to various enzymes e.g. Q53824|CAC capreomycin acetyltransferase from Streptomyces capreolus (359 aa), FASTA scores: opt: 338, E(): 1.1e-12, (33.35% identity in 363 aa overlap); Q9HXX3|CSD_PSEAE|PA3667 probable cysteine desulfurase from Pseudomonas aeruginosa (401 aa) FASTA scores: opt: 260, E(): 4.8e-08, (30.2% identity in 404 aa overlap); Q9X815|SC6G10.30 putative aminotransferase from Streptomyces coelicolor (460 aa), FASTA scores: opt: 243, E(): 5.4e-07, (29.15% identity in 374 aa overlap); Q9A761|CC1865 aminotransferase class V from Caulobacter crescentus (379 aa), FASTA scores: opt: 234, E(): 1.6e-06, (27.95% identity in 383 aa overlap); O74351|NFS1_SCHPO|SPBC21D10.11c probable cysteine desulfurase from Schizosaccharomyces pombe (Fission yeast) (498 aa), FASTA scores: opt: 232, E(): 2.5e-06, (29.1% identity in 285 aa overlap); Q9RME8|NIFS NIFS protein (cysteine desulfurase, tRNA splicing protein) from Zymomonas mobilis (370 aa), FASTA scores: opt: 230, E(): 2.6e-06, (32.85% identity in 201 aa overlap); etc. Contains PS00626 Regulator of chromosome condensation (RCC1) signature 2. O69668 No intermediary metabolism and respiration O69668 O69668 GO:0008152,GO:0030170,GO:0003824 O69668 NC_000962.3 Mycobrowser_v5 CDS 4146888 4148186 . - 0 Rv3704c gshA Involved in glutathione biosynthesis (at the first step) [catalytic activity: ATP + L-glutamate + L-cysteine = ADP + orthophosphate + gamma-L-glutamyl-L-cysteine]. Glutamate--cysteine ligase GshA (gamma-glutamylcysteine synthetase) (gamma-ECS) (GCS) (gamma-glutamyl-L-cysteine synthetase) Rv3704c, (MTV025.052c), len: 432 aa. Possible gshA, glutamate--cysteine ligase, similar to many e.g. Q9A2Z2|CC3414 glutamate--cysteine ligase from Caulobacter crescentus (453 aa), FASTA scores: opt: 404, E(): 5.9e-17, (30.45% identity in 312 aa overlap); Q9SEH0|GSH1 gamma-glutamylcysteinyl synthetase precursor from Pisum sativum (Garden pea) (499 aa), FASTA scores: opt: 400, E(): 1.1e-16, (26.4% identity in 439 aa overlap); Q9RH09|GSH gamma-glutamylcysteine synthetase from Zymomonas mobilis (462 aa), FASTA scores: opt: 397, E(): 1.6e-16, (28.95% identity in 304 aa overlap); P46309|GSH1_ARATH|GSH1|AT4G23100|F7H19.290 glutamate--cysteine ligase from Arabidopsis thaliana (Mouse-ear cress) (522 aa), FASTA scores: opt: 395, E(): 2.3e-16, (27.25% identity in 385 aa overlap); etc. But note that this putative protein is also similar to Q9JMV4|GSHA putative glutathione synthetase (fragment) from Bradyrhizobium japonicum (460 aa), FASTA scores: opt: 498, E(): 1.3e-22, (33.35% identity in 333 aa overlap) (no significant publications found (August 2001)). Nucleotide position 4147070 in the genome sequence has been corrected, A:G resulting in L373L. P9WPK7 No intermediary metabolism and respiration O69672 P9WPK7 GO:0006750,GO:0004357 6.3.2.2 P9WPK7 NC_000962.3 Mycobrowser_v5 CDS 4151180 4152217 . - 0 Rv3708c asd Involved at the second step in the common biosynthetic pathway leading from asp to the cell wall precursor MESO-diaminopimelate, to LYS, to met, to ILE and to THR [catalytic activity: L-aspartate-semialdehyde + orthophosphate + NADP(+) = L-aspartyl phosphate + NADPH]. Aspartate-semialdehyde dehydrogenase Asd (ASA dehydrogenase) (ASADH) (aspartic semialdehyde dehydrogenase) (L-aspartate-beta-semialdehyde dehydrogenase) Rv3708c, (MTV025.056c), len: 345 aa. Asd, aspartate-semialdehyde dehydrogenase (see citation below), equivalent to many e.g. P47730|DHAS_MYCBO|ASD from Mycobacterium bovis (345 aa) FASTA scores: opt: 2150, E(): 1.6e-124, (97.7% identity in 345 aa overlap); or Q9JN40|ASD from Mycobacterium bovis (323 aa), FASTA scores: opt: 2021, E(): 1.2e-116, (97.5% identity in 323 aa overlap); Q9CB78|ASD|ML2322 from Mycobacterium leprae (351 aa), FASTA scores: opt: 1889, E(): 1.6e-108, (84.45% identity in 347 aa overlap); P41404|DHAS_MYCSM|ASD from Mycobacterium smegmatis (346 aa), FASTA scores: opt: 1801, E(): 3.9e-103, (80.3% identity in 345 aa overlap); etc. Contains PS01103 Aspartate-semialdehyde dehydrogenase signature. Belongs to the aspartate-semialdehyde dehydrogenase family. P9WNX5 No intermediary metabolism and respiration 2GUL,3LLG P0A542 P9WNX5 GO:0050661,GO:0004073,GO:0005737,GO:0019877,GO:0009086,GO:0055114,GO:0046983,GO:0009088,GO:0051287 1.2.1.11 P9WNX5 NC_000962.3 Mycobrowser_v5 CDS 4152218 4153483 . - 0 Rv3709c ask Involved at the first step in the common biosynthetic pathway leading from asp to the cell wall precursor MESO-diaminopimelate, to LYS, to met, to ILE and to THR [catalytic activity: ATP + L-aspartate = ADP + 4-phospho-L-aspartate]. Possibly acts in tetramer configuration, tetramer consisting of two alpha (catalytic activity) and two beta (function not known) chains. Aspartokinase Ask (aspartate kinase) [contains: aspartokinase alpha subunit (Ask-alpha); and aspartokinase beta subunit (Ask-beta)] Rv3709c, (MTV025.057c), len: 421 aa. Ask, aspartokinase (see citation below), equivalent to Q9CB77|ask|ML2323 from Mycobacterium leprae (421 aa), FASTA scores: opt: 2531, E(): 2e-140, (92.65% identity in 421 aa overlap); and P41403|AK_MYCSM|ask from Mycobacterium smegmatis (421 aa), FASTA scores: opt: 2423, E(): 4e-134, (88.1% identity in 421 aa overlap); and to several other organisms e.g. Q9RQ25|ASKA from Amycolatopsis mediterranei (421 aa), FASTA scores: opt: 2026, E(): 5.8e-111, (72.2% identity in 421 aa overlap). Contains PS00324 Aspartokinase signature. Belongs to the aspartokinase family. Alternative products: the alpha and beta subunits of aspartokinase are produced by the use of alternative initiation sites (by similarity). P9WPX3 No intermediary metabolism and respiration P0A4Z8 P9WPX3 GO:0016597,GO:0004072,GO:0019877,GO:0005524 2.7.2.4 P9WPX3 NC_000962.3 Mycobrowser_v5 CDS 4155740 4156729 . - 0 Rv3711c dnaQ DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease [catalytic activity: N deoxynucleoside triphosphate = N diphosphate + {DNA}(N)]. Probable DNA polymerase III (epsilon subunit) DnaQ Rv3711c, (MTV025.059c), len: 329 aa. Probable dnaQ, DNA polymerase III, epsilon subunit, similar to many e.g. Q9RJ41|SCI8.12 from Streptomyces coelicolor (328 aa), FASTA scores: opt: 509, E(): 4.2e-25, (41.6% identity in 315 aa overlap); Q9JYS6|NMB1451 from Neisseria meningitidis (serogroup B) (and Q9JTR5|MA1665 from serogroup A) (470 aa), FASTA scores: opt: 247, E(): 2.6e-08, (33.15% identity in 172 aa overlap); O83649|DP3E_TREPA|DNAQ|TP0643 from Treponema pallidum (215 aa), FASTA scores: opt: 240, E(): 3.7e-08, (29.65% identity in 162 aa overlap); P03007|DP3E_ECOLI|MUTD|B0215 from Escherichia coli strain K12 (243 aa), FASTA scores: opt: 208, E(): 4.5e-06, (28.4% identity in 169 aa overlap); etc. Also similar to Q10384|YL91_MYCTU|Rv2191|MTCY190.02 from Mycobacterium tuberculosis (645 aa), FASTA scores: opt: 260, E(): 5e-09, (28.55% identity in 301 aa overlap). O69678 No information pathways O69678 O69678 GO:0006260,GO:0003887,GO:0004527,GO:0005622,GO:0003677 2.7.7.7 O69678 NC_000962.3 Mycobrowser_v5 CDS 4156981 4158222 . + 0 Rv3712 Rv3712 Function unknown; probably involved in cellular metabolism. Possible ligase Rv3712, (MTV025.060), len: 413 aa. Possible ligase , equivalent to O69522|ML2326|MLCB2407.24c hypothetical 43.8 KDA protein (possible ligase) from Mycobacterium leprae (411 aa), FASTA scores: opt: 2265, E(): 8e-129, (84.25% identity in 413 aa overlap). Also similar to ligases or hypothetical proteins e.g. Q9FCA1|2SCG58.12 putative ligase from Streptomyces coelicolor (412 aa), FASTA scores: opt: 1168, E(): 6.7e-63, (45.8% identity in 406 aa overlap); P74303|SLR0938 hypothetical 50.2 KDA protein from Synechocystis sp. strain PCC 6803 (459 aa), FASTA scores: opt: 392, E(): 3.1e-16, (28.45% identity in 397 aa overlap); Q99ZX1|SPY1035 putative UDP-N-acetylmuramyl tripeptide synthetase from Streptococcus pyogenes (445 aa), FASTA scores: opt: 335, E(): 8.1e-13, (29.2% identity in 438 aa overlap); Q9CGJ0|YLBD hypothetical protein from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (449 aa), FASTA scores: opt: 324, E(): 3.8e-12, (28.75% identity in 445 aa overlap); Q9ZGG7|MURC UDP-N-acetylmuramyl tripeptide synthetase from Heliobacillus mobilis (455 aa), FASTA scores: opt: 292, E(): 3.2e-10, (30.75% identity in 449 aa overlap); etc. I6Y4C7 No intermediary metabolism and respiration O69679 I6Y4C7 GO:0009058,GO:0016874,GO:0005524 6.-.-.- NC_000962.3 Mycobrowser_v5 CDS 4158931 4159821 . - 0 Rv3714c Rv3714c Function unknown Conserved hypothetical protein Rv3714c, (MTV025.062c), len: 296 aa. Conserved hypothetical protein, highly similar to O07396|MAV346 MAV346 protein from Mycobacterium avium (346 aa) FASTA scores: opt: 834, E(): 2.2e-46, (50.0% identity in 286 aa overlap); and also highly similar to several proteins from Mycobacterium tuberculosis e.g. O53421|Rv1073|MTV017.26 (283 aa), FASTA scores: opt: 869, E(): 1e-48, (51.1% identity in 270 aa overlap); P71763|Rv1482c|MTCY277.03c (339 aa), FASTA scores: opt: 775, E(): 1.3e-42, (46.35% identity in 289 aa overlap); P96837|Rv3555c|MTCY06G11.02c (289 aa), FASTA scores: opt: 733, E(): 5.9e-40, (44.15% identity in 281 aa overlap); etc. Partially similar to Q9Z512|UVRC_STRCO|SCC54.13c excinuclease ABC subunit C from Streptomyces coelicolor (728 aa), FASTA scores: opt: 122, E(): 2.5, (27.0% identity in 174 aa overlap). Equivalent to AAK48186 from Mycobacterium tuberculosis strain CDC1551 (341 aa) but shorter 45 aa. O69681 No conserved hypotheticals O69681 GO:0003677 O69681 NC_000962.3 Mycobrowser_v5 CDS 4195440 4195802 . + 0 Rv3744 nmtR Represses transcription from the NMTA operator-promoter. Repression is alleviated by NI(II) or co(II). Metal sensor transcriptional regulator (ArsR-SmtB family) Rv3744, (MTV025.092), len: 120 aa. Transcriptional regulator nmtR (See Cavet et al., 2002). Highly similar to many e.g. Q9ZBF4|SC9B5.26c from Streptomyces coelicolor (120 aa), FASTA scores: opt: 480, E(): 2.4e-24, (63.25% identity in 117 aa overlap); O31844|YOZA YOZA regulator from Bacillus subtilis (107 aa), FASTA scores: opt: 249, E(): 1.6e-09, (44.8% identity in 96 aa overlap); P30340|SMTB_SYNP7|SMTB from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (122 aa), FASTA scores: opt: 230, E(): 2.9e-08, (46.0% identity in 87 aa overlap); etc. Equivalent to AAK48216 from Mycobacterium tuberculosis strain CDC1551 (135 aa) but shorter 15 aa. Also similar to MTCY27_22; MTCY39_25; and MTCY441_12. Contains helix-turn-helix motif at aa 47-68 (Score 1815, +5.37 SD). Belongs to the ArsR-SmtB family of transcriptional regulators. O69711 No regulatory proteins O69711 O69711 GO:0006355,GO:0006350,GO:0003700,GO:0005622 O69711 NC_000962.3 Mycobrowser_v5 CDS 4163736 4164998 . + 0 Rv3720 Rv3720 Function unknown, but involved in lipid metabolism. Possible fatty acid synthase Rv3720, (MTV025.068), len: 420 aa. Possible fatty-acyl-phospholipid synthase, equivalent to Q9CB74|ML2334 (alias O69515|MLCB2407.16c, 439 aa) hypothetical protein from Mycobacterium leprae (420 aa) FASTA scores: opt: 2508, E(): 4.7e-153, (86.45% identity in 420 aa overlap). Also similar (especially at the C-terminus) to various fatty-acid synthases (principally cyclopropane-fatty-acyl-phospholipid synthases) and hypothetical proteins e.g. Q9KZ58|SCE25.32c putative fatty acid synthase from Streptomyces coelicolor (438 aa), FASTA scores: opt: 1101, E(): 5.5e-63, (46.1% identity in 425 aa overlap); P31049|YLP3_PSEPU hypothetical 44.7 KDA protein from Pseudomonas putida (394 aa), FASTA scores: opt: 810, E(): 2.1e-44, (46.4% identity in 293 aa overlap); Q9HT28|PA5546 hypothetical protein from Pseudomonas aeruginosa (394 aa), FASTA scores: opt: 804, E(): 5.2e-44, (40.7% identity in 371 aa overlap); Q9RSD7|DR2187 putative cyclopropane-fatty-acyl-phospholipid synthase from Deinococcus radiodurans (462 aa), FASTA scores: opt: 747, E(): 2.6e-40, (35.95% identity in 409 aa overlap); BAB50831|Q98ET6|MLL4091 cyclopropane-fatty-acyl-phospholipid synthase from Rhizobium loti (Mesorhizobium loti) (422 aa), FASTA scores: opt: 674, E(): 1.1e-35, (39.1% identity in 284 aa overlap); P30010|CFA_ECOLI|CDFA|B1661 cyclopropane-fatty-acyl-phospholip synthase from Escherichia coli strain K12 (381 aa), FASTA scores: opt: 530, E(): 1.7e-26, (33.65% identity in 312 aa overlap); etc. Also similar to other proteins from Mycobacterium tuberculosis e.g. CMA2|Rv0503c|MTCY20G9.30c (302 aa); P96911|Rv0621|MTCY20H10 (354 aa); O50416|LPQD|Rv3390|MTV004.48 (236 aa); etc. O69687 No lipid metabolism O69687 O69687 GO:0008610,GO:0008825 2.1.1.- O69687 NC_000962.3 Mycobrowser_v5 CDS 4164995 4166731 . - 0 Rv3721c dnaZX DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [catalytic activity: N deoxynucleoside triphosphate = N pyrophosphate + DNA(N)]. DNA polymerase III (subunit gamma/tau) DnaZ/X Rv3721c, (MTV025.069c), len: 578 aa. Probable dnaZX, DNA polymerase III gamma (dnaZ) and tau (dnaX), equivalent to O69514|DNAZX|ML2335 DNA polymerase III subunit gamma/tau from Mycobacterium leprae (611 aa) FASTA scores: opt: 2344, E(): 4.7e-118, (78.75% identity in 602 aa overlap). Also highly similar to many e.g. Q9RKL5|DNAZ from Streptomyces coelicolor (784 aa) FASTA scores: opt: 1755, E(): 1.8e-86, (59.55% identity in 435 aa overlap); Q9KGM4|DNAX|BH0034 from Bacillus halodurans (564 aa), FASTA scores: opt: 946, E(): 2.5e-43, (37.4% identity in 460 aa overlap); P09122|DP3X_BACSU|DNAX|DNAH from Bacillus subtilis (563 aa), FASTA scores: opt: 841, E(): 1e-37, (30.8% identity in 510 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). P9WNT9 No information pathways P63975 P9WNT9 GO:0003677,GO:0009360,GO:0006260,GO:0003887,GO:0017111,GO:0005524 2.7.7.7 P9WNT9 NC_000962.3 Mycobrowser_v5 CDS 4169467 4169709 . + 0 Rv3724A cut5a Hydrolysis of cutin (a polyester that forms the structure of plant cuticle). Probable cutinase precursor [first part] Cut5a Rv3724A, (MTV025.072), len: 80 aa. Probable cut5a, truncated cutinase precursor, similar to N-terminal end of others e.g. Q9KK87 serine esterase cutinase from Mycobacterium avium (220 aa), FASTA scores: opt: 202, E(): 1.5e-06, (56.45% identity in 62 aa overlap); Q9XB09|RVD2-RV1758 protein (fragment) from Mycobacterium bovis BCG (143 aa), FASTA scores: opt: 200, E(): 1.5e-06, (61.4% identity in 57 aa overlap); and Q00298|CUTI_BOTCI|CUTA cutinase precursor from Botrytis cinerea (Botryotinia fuckeliana) (202 aa), FASTA scores: opt: 108, E(): 2.2, (40.4% identity in 52 aa overlap). Also highly similar to others from Mycobacterium tuberculosis e.g. O06318|CUT3_MYCTU|Rv3451|MT3557|MTCY13E12.04 probable cutinase precursor (247 aa), FASTA scores: opt: 189, E(): 1.2e-05, (58.0% identity in 50 aa overlap); Q50664|CUT2_MYCTU|Rv2301|MT2358|MTCY339.08c probable cutinase precursor (219 aa), FASTA scores: opt: 172, E(): 0.00015, (59.2% identity in 49 aa overlap); O06793|Rv1758|MTCY28.24|Z95890 hypothetical 17.9 KDA protein (174 aa), FASTA scores: opt: 641, E(): 2.7e-29, (57.2% identity in 166 aa overlap); O06319|Rv3452|MTY13E12.05; and U00015_11 from Mycobacterium leprae. Belongs to the cutinase family. Rest of cutinase ORF continues as Rv3724B|CUT5B, frameshifting could occur near position 4169668. Sequence has been checked but no errors found. Q79FA5 No cell wall and cell processes Q79FA5 Q79FA5 GO:0016787,GO:0008152 3.1.1.- Q79FA5 NC_000962.3 Mycobrowser_v5 CDS 4172955 4174763 . + 0 Rv3727 Rv3727 Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv3727, (MTV025.075), len: 602 aa. Possible oxidoreductase, similar to several plants phytoene dehydrogenases/desaturases e.g. Q9HSE1|CRTI3|VNG0277G phytoene dehydrogenase from Halobacterium sp. strain NRC-1 (541 aa), FASTA scores: opt: 299, E(): 1.1e-10, (29.85% identity in 576 aa overlap); Q9FZL6|CITPDS1 phytoene desaturase from Citrus unshiu (Satsuma orange) (553 aa), FASTA scores: opt: 164, E(): 0.018, (24.2% identity in 434 aa overlap); Q07356|CRTI_ARATH|PDS|AT4G14210|DL3145c phytoene dehydrogenase precursor from Arabidopsis thaliana (Mouse-ear cress) (566 aa), FASTA scores: opt: 163, E(): 0.021, (23.95% identity in 434 aa overlap); etc. N-terminal end similar to O69871|SC1C3.29 putative protoporphyrinogen oxidase (fragment) from Streptomyces coelicolor (61 aa), FASTA scores: opt: 154, E(): 0.012, (60.45% identity in 43 aa overlap). The region between aa 155-310 is highly similar to Q49778|B2126_C1_169 from Mycobacterium leprae (159 aa), FASTA scores: opt: 437, E(): 1.5e-19, (46.6% identity in 161 aa overlap). And the region between aa 462-546 is highly similar to the N-terminal end of Q50003|U1764T from Mycobacterium leprae (155 aa), FASTA scores: opt: 277, E(): 8.3e-10, (57.65% identity in 85 aa overlap). O69694 No intermediary metabolism and respiration O69694 O69694 GO:0016491,GO:0055114 1.-.-.- NC_000962.3 Mycobrowser_v5 CDS 4181758 4182834 . + 0 Rv3731 ligC This protein seals during DNA replication, DNA recombination and DNA repair NICKS in double-stranded DNA [catalytic activity: ATP + (deoxyribonucleotide)(N) + (deoxyribonucleotide)(M) = AMP + pyrophosphate + (deoxyribonucleotide)(N+M)]. Possible ATP-dependent DNA ligase LigC (polydeoxyribonucleotide synthase [ATP]) (polynucleotide ligase [ATP]) (sealase) (DNA repair protein) (DNA joinase) Rv3731, (MTV025.079), len: 358 aa. Possible ligC, DNA ligase ATP-dependent (see citation below), similar to numerous archaebacterial and eukaryotic polynucleotide DNA ligases e.g. Q9XAM3|SC4C6.17c from Streptomyces coelicolor (355 aa), FASTA scores: opt: 1429, E(): 1.7e-82, (60.4% identity in 361 aa overlap); BAB54870|MLL9685 from Rhizobium loti (Mesorhizobium loti) plasmid pMLb (337 aa), FASTA scores: opt: 667, E(): 1.2e-34, (40.35% identity in 347 aa overlap); Q9HH07|DNLI_THEFM|LIG from Thermococcus fumicolans (559 aa), FASTA scores: opt: 335, E(): 1.4e-13, (27.25% identity in 330 aa overlap); O59288|DNLI_PYRHO from Pyrococcus horikoshii (559 aa), FASTA scores: opt: 307, E(): 8e-12, (26.85% identity in 272 aa overlap); etc. Also similar to Rv3062|MTCY22D7_19c|LIGB probable DNA ligase from Mycobacterium tuberculosis (507 aa), FASTA score: (30.3% identity in 356 aa overlap). Seems to belong to the ATP-dependent DNA ligase family. L0TDE1 No information pathways O69698 L0TDE1 GO:0003910,GO:0006310,GO:0006281,GO:0006260,GO:0005524 6.5.1.1 L0TDE1 NC_000962.3 Mycobrowser_v5 CDS 4184526 4185890 . - 0 Rv3734c tgs2 May be involved in synthesis of triacylglycerol Putative triacylglycerol synthase (diacylglycerol acyltransferase) Tgs2 Rv3734c, (MTV025.082c), len: 454 aa. Putative tgs2, triacylglycerol synthase (See Daniel et al., 2004), highly similar to O69707|Y1E0_MYCTU|Rv3740c|MT3848|MTV025.088c hypothetical protein from Mycobacterium tuberculosis (448 aa), FASTA scores: opt: 1917, E(): 1.3e-111, (61.4% identity in 451 aa overlap); and similar to many other proteins from Mycobacterium tuberculosis (strains H37Rv and CDC1551) e.g. P71694|YE43_MYCTU|Rv1425|MT1468|MTCY21B4.43|MTCY493.29c (459 aa), FASTA scores: opt: 824, E(): 1.1e-43, (36.5% identity in 460 aa overlap); Q50680|YM85_MYCTU|Rv2285|MT2343|MTCY339.25c (445 aa) FASTA scores: opt: 766, E(): 4.1e-40, (36.4% identity in 453 aa overlap); etc. Also similar to Q9RIU8|SCM11.13c hypothetical 47.1 KDA protein from Streptomyces coelicolor (446 aa), FASTA scores: opt: 331, E(): 4.3e-13, (32.9% identity in 468 aa overlap); and Q9X7A8|ML1244|MLCB1610.05 conserved membrane protein from Mycobacterium leprae (491 aa), FASTA scores: opt: 296, E(): 7e-11, (28.35% identity in 413 aa overlap). Contains PS00339 Aminoacyl-transfer RNA synthetases class-II signature 2. Start site chosen by homology, but may extend further upstream to 93257. P9WKC7 No lipid metabolism P67210 P9WKC7 GO:0006071,GO:0008610,GO:0047196,GO:0004144 2.3.1.20,2.3.1.75 NC_000962.3 Mycobrowser_v5 CDS 4186634 4187695 . + 0 Rv3736 Rv3736 Involved in transcriptional mechanism. Transcriptional regulatory protein (probably AraC/XylS-family) Rv3736, (MTV025.084), len: 353 aa. Probable transcriptional regulator, araC/xylS family, similar to many transcriptional regulators and hypothetical proteins e.g. CAC38740 hypothetical 35.4 KDA protein from Bradyrhizobium japonicum (318 aa), FASTA scores: opt: 438, E(): 2e-20, (29.4% identity in 306 aa overlap); Q9HZ25|PA3215 probable transcriptional regulator from Pseudomonas aeruginosa (337 aa), FASTA scores: opt: 395, E(): 1.1e-17, (30.3% identity in 320 aa overlap); Q9HTN1|PA5324 probable transcriptional regulator from Pseudomonas aeruginosa (356 aa), FASTA scores: opt: 313, E(): 1.8e-12, (25.85% identity in 329 aa overlap); Q9Z3Y6|PHBR transcriptional regulator PHBR from Pseudomonas sp. 61-3 (379 aa), FASTA scores: opt: 271, E(): 8.3e-10, (22.95% identity in 357 aa overlap); etc. Also highly similar to Q06861|VIRS_MYCTU|Rv3082c|MTV013.03c possible virulence-regulating protein from Mycobacterium tuberculosis (340 aa), FASTA scores: opt: 656, E(): 3.7e-34, (36.95% identity in 333 aa overlap); and similar to other hypothetical mycobacterial proteins e.g. P71663|YD95_MYCTU|Rv1395|MT1440|MTCY21B4.12 (344 aa). Contains helix-turn-helix motif at aa 245-266 (Score 1140, +3.07 SD). Seems belong to the AraC/XylS family of transcriptional regulators. O69703 No regulatory proteins O69703 O69703 GO:0006355,GO:0043565,GO:0006350,GO:0003700,GO:0005622 O69703 NC_000962.3 Mycobrowser_v5 CDS 4200421 4201326 . + 0 Rv3754 tyrA Involved in tyrosine biosynthesis [catalytic activity: prephenate + NAD(+) = 4-hydroxyphenylpyruvate + CO(2) + NADH]. Prephenate dehydrogenase TyrA (PDH) (hydroxyphenylpyruvate synthase) Rv3754, (MTV025.102), len: 301 aa. Probable tyrA, prephenate dehydrogenase, equivalent, but shorter 27 aa, to Q9CB34|ML2472 possible prephenate dehydrogenase from Mycobacterium leprae (327 aa) FASTA scores: opt: 1600, E(): 1.6e-89, (80.0% identity in 300 aa overlap). Also similar to many pephenate dehydrogenases e.g. Q9RND8|TYRA from Bordetella bronchiseptica (Alcaligenes bronchisepticus) (299 aa), FASTA scores: opt: 345, E(): 9.7e-14, (32.85% identity in 271 aa overlap); Q9RVA7|DR1122 from Deinococcus radiodurans (372 aa) FASTA scores: opt: 341, E(): 2e-13, (35.65% identity in 216 aa overlap); P20692|TYRA_BACSU from Bacillus subtilis (372 aa), FASTA scores: opt: 314, E(): 8.6e-12, (27.75% identity in 263 aa overlap); etc. Also similar to Q04983|TYRC_ZYMMO TYRC protein [includes: cyclohexadienyl dehydrogenase and prephenate dehydrogenase activities] from Zymomonas mobilis (293 aa), FASTA scores: opt: 290, E(): 2e-10, (30.15% identity in 239 aa overlap). Equivalent to AAK48225 from Mycobacterium tuberculosis strain CDC1551 (323 aa) but shorter 22 aa. O69721 No intermediary metabolism and respiration O69721 O69721 GO:0055114,GO:0004665,GO:0008977,GO:0006571 1.3.1.12 O69721 NC_000962.3 Mycobrowser_v5 CDS 4201894 4202613 . - 0 Rv3756c proZ Thought to be involved in active transport of osmoprotectant (glycine betaine/carnitine/choline/L-proline) across the membrane (import). Responsible for the translocation of the substrate across the membrane. Possible osmoprotectant (glycine betaine/carnitine/choline/L-proline) transport integral membrane protein ABC transporter ProZ Rv3756c, (MTV025.104c), len: 239 aa. Possible proZ, osmoprotectant transport integral membrane protein ABC transporter (see citation below), similar to osmoprotection proteins (proW, proZ) involved in glycine betaine/L-proline/choline transport, e.g. BAB58609|Q99RI4|OPUCB|SA2236|SAV2447 OPUCB protein (probable glycine betaine/carnitine/choline ABC transporter) from Staphylococcus aureus (211 aa) FASTA scores: opt: 434, E(): 2.5e-18, (36.6% identity in 194 aa overlap); Q45461|OPBB_BACSU|OPUBB|prow choline transport system permease protein (mediate the uptake of choline for synthesis of the osmoprotectant glycine betaine) from Bacillus subtilis (217 aa), FASTA scores: opt: 402, E(): 1.9e-16, (32.0% identity in 203 aa overlap); O34878|OPCB_BACSU|OPUCB glycine betaine/carnitine/choline transport system permease protein from Bacillus subtilis (217 aa), FASTA scores: opt: 385, E(): 1.8e-15, (30.2% identity in 222 aa overlap); P39775|O34657|OPUBD|PROZ|OPBD_BACSU choline transport system permease protein from Bacillus subtilis (226 aa) FASTA scores: opt: 350, E(): 2e-13, (31.75% identity in 208 aa overlap); etc. Could belong to the CYSTW subfamily. O69722 No virulence, detoxification, adaptation O69722 O69722 GO:0005886,GO:0006810,GO:0005215,GO:0016021 O69722 NC_000962.3 Mycobrowser_v5 CDS 4203287 4204417 . - 0 Rv3758c proV Thought to be involved in active transport of osmoprotectant (glycine betaine/carnitine/choline/L-proline) across the membrane (import). Responsible for energy coupling to the transport system. Possible osmoprotectant (glycine betaine/carnitine/choline/L-proline) transport ATP-binding protein ABC transporter ProV Rv3758c, (MTV025.106c), len: 376 aa. Possible proV, osmoprotectant transport ATP-binding protein ABC transporter (see citation below), highly similar to osmoprotection proteins (proV) involved in glycine betaine/L-proline/choline transport, e.g. BAB58610|Q99RI3|OPUCA|SA2237|SAV2448 glycine betaine/carnitine/choline ABC transporter (ATP-binding) from Staphylococcus aureus (410 aa), FASTA scores: opt: 816, E(): 8.4e-39, (39.5% identity in 362 aa overlap); O34992|OPCA_BACSU|OPUCA glycine betaine/carnitine/choline transport ATP-binding protein from Bacillus subtilis (380 aa), FASTA scores: opt: 807, E(): 2.5e-38, (40.55% identity in 333 aa overlap); Q45460|OPBA_BACSU|OPUBA|prov choline transport ATP-binding protein from Bacillus subtilis (381 aa), FASTA scores: opt: 801, E(): 5.6e-38, (40.65% identity in 337 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporter family signature. Belongs to the ATP-binding transport protein family (ABC transporters). O69724 No virulence, detoxification, adaptation O69724 O69724 GO:0016887,GO:0005524 O69724 NC_000962.3 Mycobrowser_v5 CDS 4206996 4208876 . - 0 Rv3762c Rv3762c Function unknown; probably involved in cellular metabolism. Possible hydrolase Rv3762c, (MTV025.110c), len: 626 aa. Possible hydrolase, highly similar to hypothetical proteins and beta-lactamases e.g. Q9RL04|SC5G9.23 hypothetical 70.3 KDA protein from Streptomyces coelicolor (648 aa), FASTA scores: opt: 2088, E(): 3.7e-124, (52.9% identity in 624 aa overlap); P32717|YJCS_ECOLI|B4083 hypothetical 73.2 KDA protein from Escherichia coli strain K12 (661 aa), FASTA scores: opt: 1911, E(): 5.7e-113, (46.9% identity in 631 aa overlap); Q9A824|CC1540 metallo-beta-lactamase family protein from Caulobacter crescentus (647 aa), FASTA scores: opt: 1891, E(): 1e-111, (48.55% identity in 628 aa overlap); Q08347|YOL164W chromosome xv reading frame ORF from Saccharomyces cerevisiae (Baker's yeast) (646 aa) FASTA scores: opt: 1829, E(): 8.4e-108, (45.7% identity in 615 aa overlap); Q9I5I9|PA0740 probable beta-lactamase from Pseudomonas aeruginosa (658 aa), FASTA scores: opt: 1699, E(): 1.4e-99, (43.15% identity in 630 aa overlap); Q52556|SDSA alkyl sulfatase (protein involved in the degradation of sulfate esters of long-chain primaryal cohols e.g. SDS sodium dodecyl sulfate) from Pseudomonas sp (528 aa), FASTA scores: opt: 841, E(): 1.7e-45, (33.7% identity in 534 aa overlap); etc. N-terminual end also highly similar to Q48790|SEPA SEPA protein (protein implicated in cell separation) from Listeria monocytogenes (391 aa), FASTA scores: opt: 1256, E(): 8.3e-72, (49.6% identity in 363 aa overlap). Also slight similarity to P96253|Rv0407|MTCY22G10.03 hypothetical 37.0 KDA protein from Mycobacterium tuberculosis (336 aa). O69728 No intermediary metabolism and respiration O69728 GO:0016787 3.-.-.- O69728 NC_000962.3 Mycobrowser_v5 CDS 4212996 4213940 . - 0 Rv3767c Rv3767c Possible methyltransferase Possible S-adenosylmethionine-dependent methyltransferase Rv3767c, (MTV025.115c, MTCY13D12.01), len: 314 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), similar to other Mycobacterium tuberculosis hypothetical proteins e.g. P96823|Rv0146|MTCI5.20 34.0 KDA protein (310 aa), FASTA scores: opt: 909, E(): 5.3e-50, (48.1% identity in 316 aa overlap); O53686|Rv0281|MTV035.09 (302 aa), FASTA scores: opt: 802, E(): 2.8e-43, (45.2% identity in 314 aa overlap); Q50726|YX99_MYCTU|Rv3399|MT3507|MTCY78.29c (348 aa), FASTA scores: opt: 796, E(): 7.6e-43, (45.35% identity in 302 aa overlap); MTCY78_30; MTCY31_23; MTCY210_45; MTCY4C12_14; MTY13D12_21, MTCI5_19; MTCY180_22; etc. Contains probable N-terminal signal sequence P9WFH5 No lipid metabolism O86359 P9WFH5 GO:0008168 2.1.1.- P9WFH5 NC_000962.3 Mycobrowser_v5 CDS 4217134 4218195 . + 0 Rv3772 hisC2 Involved in histidine biosynthetic pathway (at the eighth step) [catalytic activity: L-histidinol-phosphate + 2-oxoglutarate = 3-(imidazol-4-YL)-2-oxopropyl phosphate + glutamate]. Probable histidinol-phosphate aminotransferase HisC2 (imidazole acetol-phosphate transaminase) (imidazolylacetolphosphate aminotransferase) Rv3772, (MTCY13D12.06), len: 353 aa. Probable hisC2, histidinol-phosphate aminotransferase, highly similar to Q9ZBY8|SCD78.11 putative histidinol-phophate aminotransferase from Streptomyces coelicolor (359 aa), FASTA scores: opt: 1165, E(): 7.1e-64, (52.55% identity in 356 aa overlap); and similar to many e.g. Q9EYX2 from Gardnerella vaginalis (317 aa) FASTA scores: opt: 814, E(): 1.7e-42, (45.15% identity in 308 aa overlap); Q9CMI7|HISH_1PM0838|HISH from Pasteurella multocida (365 aa), FASTA scores: opt: 701, E(): 1.5e-35, (35.05% identity in 351 aa overlap); O07131|HIS8_METFL|HISC|HISH from Methylobacillus flagellatum (368 aa), FASTA scores: opt: 645, E(): 4e-32, (34.5% identity in 345 aa overlap); etc. Contains PS00599 Aminotransferases class-II pyridoxal-phosphate attachment site. Belongs to class-II of pyridoxal-phosphate-dependent aminotransferases. Cofactor: pyridoxal phosphate. P9WML5 No intermediary metabolism and respiration P72039 P9WML5 GO:0004400,GO:0030170,GO:0000105 2.6.1.- P9WML5 NC_000962.3 Mycobrowser_v5 CDS 4329417 4329614 . - 0 Rv3857c Rv3857c Unknown Possible membrane protein Rv3857c, (MTCY01A6.11), len: 65 aa. Possible membrane protein. P96220 No cell wall and cell processes P96220 NC_000962.3 Mycobrowser_v5 CDS 4219685 4220932 . + 0 Rv3775 lipE Function unknown; lipolytic enzyme probably involved in cellular metabolism. Probable lipase LipE Rv3775, (MTCY13D12.09), len: 415 aa. Probable lipE, hydrolase lipase, equivalent to Q9CD95|LIPE|ML0119 probable hydrolase from Mycobacterium leprae (411 aa), FASTA scores: opt: 2418, E(): 6.4e-144, (84.75% identity in 406 aa overlap). Also similar to other esterases e.g. Q9ABH2|CC0255 esterase a from Caulobacter crescentus (374 aa), FASTA scores: opt: 427, E(): 2.4e-19, (28.9% identity in 391 aa overlap); O87861|ESTA esterase a from Streptomyces chrysomallus (389 aa), FASTA scores: opt: 417, E(): 1e-18, (31.0% identity in 361 aa overlap); Q9RK50|SCF12.08 putative esterase from Streptomyces coelicolor (376 aa), FASTA scores: opt: 385, E(): 1e-16, (31.35% identity in 373 aa overlap); etc. Also similar to proteins from Mycobacterium tuberculosis e.g. P71778|Rv1497|MTCY277.19 hypothetical 45.8 KDA protein (429 aa), FASTA scores: opt: 457, E(): 3.5e-21, (30.4% identity in 395 aa overlap). P72041 No intermediary metabolism and respiration P72041 P72041 GO:0016787 3.1.-.- P72041 NC_000962.3 Mycobrowser_v5 CDS 4222694 4223680 . + 0 Rv3777 Rv3777 Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv3777, (MTCY13D12.11), len: 328 aa. Probable oxidoreductase, equivalent to Q9CD96|ML0118 putative oxidoreductase from Mycobacterium leprae (336 aa) FASTA scores: opt: 1661, E(): 1.1e-87, (76.0% identity in 325 aa overlap). Also highly similar to many e.g. Q9XA55|SCGD3.24c putative quinone oxidoreductase from Streptomyces coelicolor (326 aa) FASTA scores: opt: 1118, E(): 1.3e-64, (59.6% identity in 312 aa overlap); O65423|F18E5.200|F17L22.40|AT4G21580 putative NADPH quinone oxidoreductase from Arabidopsis thaliana (Mouse-ear cress) (325 aa), FASTA scores: opt: 1110, E(): 3e-56, (52.15% identity in 326 aa overlap); Q98FI0|MLL3767 NADPH quinone oxidoreductase from Rhizobium loti (Mesorhizobium loti) (326 aa), FASTA scores: opt: 980, E(): 7.9e-49, (47.85% identity in 324 aa overlap); etc. P72043 No intermediary metabolism and respiration P72043 P72043 GO:0016491,GO:0008270,GO:0055114 1.-.-.- P72043 NC_000962.3 Mycobrowser_v5 CDS 4223699 4224895 . - 0 Rv3778c Rv3778c Function unknown; probably involved in cellular metabolism. Possible aminotransferase Rv3778c, (MTCY13D12.12c), len: 398 aa. Possible aminotransferase, equivalent to Q9CD97|ML0117 hypothetical protein from Mycobacterium leprae (398 aa) FASTA scores: opt: 2141, E(): 1.2e-123, (83.4% identity in 398 aa overlap). Also similar to other aminotransferases and cysteine desulfurases e.g. Q9K3K6|SCG20A.34 putative aminotransferase from Streptomyces coelicolor (400 aa), FASTA scores: opt: 723, E(): 6.5e-37, (36.3% identity in 402 aa overlap); Q9KSS2|VC1184 NIFS-related protein (aminotransferase-related) from Vibrio cholerae (416 aa) FASTA scores: opt: 595, E(): 4.5e-29, (31.35% identity in 405 aa overlap); Q98NK4|MLR0102 aminotransferase from Rhizobium loti (Mesorhizobium loti) (425 aa), FASTA scores: opt: 563, E(): 4.2e-27, (29.4% identity in 408 aa overlap); Q9RY03|DR0151 NIFS-related protein from Deinococcus radiodurans (401 aa), FASTA scores: opt: 484, E(): 2.7e-22, (32.35% identity in 399 aa overlap); Q9A766|CC1860 aminotransferase class V from Caulobacter crescentus (408 aa), FASTA scores: opt: 390, E(): 1.5e-16, (27.85% identity in 413 aa overlap); etc. P9WQ67 No intermediary metabolism and respiration 3CAI P72044 P9WQ67 GO:0030170,GO:0008483,GO:0008152 2.6.1.- P9WQ67 NC_000962.3 Mycobrowser_v5 CDS 4227529 4228350 . + 0 Rv3781 rfbE May form an ATP-driven O-antigen/lipopolysaccharide export apparatus, in association with RFBD|Rv3783. Responsible for energy coupling to the transport system. Probable O-antigen/lipopolysaccharide transport ATP-binding protein ABC transporter RfbE Rv3781, (MTCY13D12.15), len: 273 aa. Probable rfbE, polysaccharide-transport ATP-binding protein ABC transporter, involved in O-antigen/lipopolysaccharides (LPS) transport (see Braibant et al., 2000), equivalent to Q9CDA0|ML0114 putative ABC transporter ATP-binding component from Mycobacterium leprae (272 aa), FASTA scores: opt: 1581, E(): 3e-83, (91.4% identity in 267 aa overlap). Also highly similar to AAK71283 LPS/O-antigen export permease from Coxiella burnetii (258 aa), FASTA scores: opt: 793, E(): 2.5e-38, (45.45% identity in 253 aa overlap); Q9PAF0|XF2568 ABC transporter ATP-binding protein from Xylella fastidiosa (246 aa), FASTA scores: opt: 758, E(): 2.4e-36, (47.75% identity in 243 aa overlap); Q56903|RFBE_YEREN O-antigen export system ATP-binding protein from Yersinia enterocolitica (239 aa) (see Zhang et al., 1993), FASTA scores: opt: 697, E(): 7e-33, (48.65% identity in 224 aa overlap); Q50863|RFBB_MYXXA O-antigen export system ATP-binding from Myxococcus xanthus (437 aa), FASTA scores: opt: 605, E(): 2e-27, (42.05% identity in 207 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters). P72047 No cell wall and cell processes P72047 P72047 GO:0016887,GO:0005524 P72047 NC_000962.3 Mycobrowser_v5 CDS 4230256 4231236 . + 0 Rv3784 Rv3784 Possibly involved in dTDP-L-rhamnose biosynthesis within the O antigen biosynthesis pathway of lipopolysaccharide biosynthesis [catalytic activity: dTDP-glucose = dTDP-4-dehydro-6-deoxy-D-glucose + H(2)O]. Possible dTDP-glucose 4,6-dehydratase Rv3784, (MTCY13D12.18), len: 326 aa. Possible dTDP-glucose 4,6-dehydratase, but experimental study shown that the purified protein didn't have dTDP-glucose dehydratase (rmlB) activity (see citation below). Similar to others e.g. Q9YCT1|APE1180 long hypothetical dTDP-glucose 4,6-dehydratase from Aeropyrum pernix (330 aa) FASTA scores: opt: 598, E(): 3.7e-30, (34.9% identity in 315 aa overlap); O27817|MTH1789 dTDP-glucose 4,6-dehydratase from Methanothermobacter thermautotrophicus (336 aa) FASTA scores: opt: 587, E(): 1.8e-29, (34.9% identity in 315 aa overlap); Q9X5W0|GRSE TDP-glucose-4,6-dehydratase homolog from Streptomyces griseus (324 aa), FASTA scores: opt: 583, E(): 3.2e-29, (35.7% identity in 325 aa overlap); Q9K7J7|SPSJ|BH3364 spore coat polysaccharide synthesis (dTDP glucose 4, 6-dehydratase) from Bacillus halodurans (321 aa), FASTA scores: opt: 562, E(): 6.5e-28, (33.0% identity in 318 aa overlap); Q9UZH2|RFBB|PAB0785 dTDP-glucose 4,6-dehydratase from Pyrococcus abyssi (333 aa), FASTA scores: opt: 552, E(): 2.8e-27, (33.95% identity in 318 aa overlap); P27830|RFFG_ECOLI|B3788 dTDP-glucose 4,6-dehydratase from Escherichia coli strain K12 (355 aa), FASTA scores: opt: 401, E(): 7.5e-28, (31.3% identity in 348 aa overlap); etc. But also similar to several UDP-glucose 4-epimerases and other proteins e.g. O59375|PH1742 long hypothetical UDP-glucose 4-epimerase from Pyrococcus horikoshii (306 aa) FASTA scores: opt: 600, E(): 2.6e-30, (34.5% identity in 313 aa overlap); Q9ZGC7|LANH14 NDP-hexose 4,6-dehydratase HOMOLOGfrom Streptomyces cyanogenus (326 aa), FASTA scores: opt: 593, E(): 7.6e-30, (36.45% identity in 321 aa overlap); Q57664|GALE_METJA|MJ0211 putative UDP-glucose 4-epimerase from Methanococcus jannaschii (305 aa) FASTA scores: opt: 575, E(): 9.6e-29, (32.6% identity in 313 aa overlap); etc. Seems to belong to the sugar epimerase family, dTDP-glucose dehydratase subfamily. Note that previously known as epiB. P72050 No intermediary metabolism and respiration P72050 P72050 GO:0050662,GO:0008460,GO:0044237 4.2.1.46 P72050 NC_000962.3 Mycobrowser_v5 CDS 4235779 4237164 . + 0 Rv3790 dprE1 Together with DPRE2|Rv3791, catalyzes epimerization of decaprenylphosphoryl ribose (DPR) to decaprenylphosphoryl arabinose (DPA) in arabinan synthesis Decaprenylphosphoryl-beta-D-ribose 2'-oxidase Rv3790, (MTCY13D12.24), len: 461 aa. DprE1, decaprenylphosphoryl-beta-D-ribose 2'-oxidase, equivalent to Q9CDA4|ML0109 putative FAD-linked oxidoreductase from Mycobacterium leprae (460 aa), FASTA scores: opt: 2722, E(): 1.4e-161, (86.55% identity in 461 aa overlap). Also highly similar to others e.g. Q9KZA4|SC5G8.10c putative oxidoreductase from Streptomyces coelicolor (457 aa), FASTA scores: opt: 1336, E(): 1.7e-75, (47.1% identity in 452 aa overlap); Q98KY4|MLL1265 probable oxidoreductase from Rhizobium loti (Mesorhizobium loti) (449 aa), FASTA scores: opt: 636, E(): 4.9e-32, (36.0% identity in 439 aa overlap); Q9HDX8|SPAPB1A10.12c putative D-arabinono-1,4-lactone oxidase from Schizosaccharomyces pombe (Fission yeast) (461 aa), FASTA scores: opt: 297, E(): 5.6e-11, (23.55% identity in 467 aa overlap); etc. C-terminal end has a high similarity to Q9AQD0 putative oxidoreductase (fragment) from Mycobacterium smegmatis (149 aa) FASTA scores: opt: 901, E(): 6.5e-49, (86.6% identity in 149 aa overlap). Identified as the target of antimicrobial agent 1,3-benzothiazin-4-ones (BTZs) (See Makarov et al., 2009). P9WJF1 No lipid metabolism P72056 P9WJF1 GO:0050660,GO:0007047,GO:0016020,GO:0055114,GO:0003885 1.-.-.- P9WJF1 NC_000962.3 Mycobrowser_v5 CDS 4237165 4237929 . + 0 Rv3791 dprE2 Together with DPRE2|Rv3791, catalyzes epimerization of decaprenylphosphoryl ribose (DPR) to decaprenylphosphoryl arabinose (DPA) in arabinan synthesis Decaprenylphosphoryl-D-2-keto erythro pentose reductase Rv3791, (MTCY13D12.25), len: 254 aa. DprE2, decaprenylphosphoryl-D-2-keto erythro pentose reductase, equivalent to Q9CDA5|ML0108 putative oxidoreductase from Mycobacterium leprae (254 aa), FASTA scores: opt: 1458, E(): 1.6e-83, (89.0% identity in 254 aa overlap); and O05764 putative protein belonging to the short-chain alcohol dehydrogenase from Mycobacterium smegmatis (254 aa), FASTA scores: opt: 1412, E(): 1.2e-80, (85.05% identity in 254 aa overlap). Also highly similar to Q9KZA5|SC5G8.09c putative short-chain dehydrogenase from Streptomyces coelicolor (256 aa), FASTA scores: opt: 733, E(): 1.8e-38, (45.3% identity in 254 aa overlap); and P43168|YMP3_STRCO hypothetical oxidoreductase from Streptomyces coelicolor (251 aa), FASTA scores: opt: 623, E(): 1.2e-31, (42.15% identity in 254 aa overlap); and similar to various oxidoreductases (principally acetoacetyl-CoA reductases) e.g. P14697|PHBB_ALCEU acetoacetyl-CoA reductase (246 aa) from Alcaligenes eutrophus (Ralstonia eutropha) (246 aa) FASTA scores: opt: 264, E(): 2.3e-09, (29.9% identity in 204 aa overlap); P45375|PHBB_CHRVI acetoacetyl-CoA reductase from Chromatium vinosum (246 aa), FASTA scores: opt: 261, E(): 3.5e-09, (27.45% identity in 226 aa overlap); Q9RT30|DR1938 oxidoreductase (short-chain dehydrogenase/reductase family) from Deinococcus radiodurans (283 aa), FASTA scores: opt: 251, E(): 1.7e-08, (27.55% identity in 236 aa overlap); etc. Also similar to Q10681|YK73_MYCTU|Rv2073c|MT2133|MTCY49.12 putative short-chain type dehydrogenase/reductase from Mycobacterium tuberculosis (249 aa), FASTA scores: opt: 589, E(): 1.5e-29, (41.25% identity in 252 aa overlap). Contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. P9WGS9 No lipid metabolism P66783 P9WGS9 GO:0007047,GO:0055114,GO:0016491 1.1.1.- P9WGS9 NC_000962.3 Mycobrowser_v5 CDS 4237932 4239863 . + 0 Rv3792 aftA Involved in the biosynthesis of the mycobacterial cell wall arabinan Arabinofuranosyltransferase AftA Rv3792, (MTCY13D12.26), len: 643 aa. aftA, arabinofuranosyltransferase (See Alderwick et al., 2006). Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). Probable conserved transmembrane protein, equivalent, but longer 21 aa, to Q9CDA6|ML0107 putative membrane protein from Mycobacterium leprae (632 aa), FASTA scores: opt: 1981, E(): 2.1e-110, (77.5% identity in 631 aa overlap). C-terminal end highly similar to C-terminus of O05765 putative product ORF 3 from Mycobacterium smegmatis (603 aa), FASTA scores: opt: 1261, E(): 1.4e-67, (70.7% identity in 266 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WN03 No cell wall and cell processes P72058 P9WN03 GO:0007047,GO:0016021,GO:0005886,GO:0046677,GO:0016757,GO:0044038 2.4.2.- P9WN03 NC_000962.3 Mycobrowser_v5 CDS 4243233 4246517 . + 0 Rv3794 embA Involved in the biosynthesis of the mycobacterial cell wall arabinan and resistance to ethambutol (EMB; dextro-2,2'-(ethylenediimino)-DI-1-butanol). Polymerizes arabinose into the arabinan of arabiogalactan [catalytic activity: UDP-L-arabinose + indol-3-ylacetyl-myo-inositol = UDP + indol-3-ylacetyl-myo-inositol L-arabinoside]. Integral membrane indolylacetylinositol arabinosyltransferase EmbA (arabinosylindolylacetylinositol synthase) Rv3794, (MTCY13D12.28), len: 1094 aa. EmbA, integral membrane protein, indolylacetylinositol arabinosyltransferase (see citations below), equivalent to P71485|EMBA arabinosyl transferase from Mycobacterium avium (1108 aa), FASTA scores: opt: 5024, E(): 0, (81.9% identity in 1109 aa overlap); Q9CDA8|EMBA|ML0105 putative arabinosyl transferase from Mycobacterium leprae (1111 aa), FASTA scores: opt: 4782, E(): 0, (78.6% identity in 1111 aa overlap); Q50394|EMBA putative arabinosyl transferase from Mycobacterium smegmatis (1092 aa), FASTA scores: opt: 4100, E(): 0, (67.4% identity in 1092 aa overlap). Also similar to Q9CDA7|EMBC|ML0106 putative arabinosyl transferase from Mycobacterium leprae (1070 aa), FASTA scores: opt: 1933, E(): 1.5e-100, (40.6% identity in 1108 aa overlap); Q50393|EMBC putative arabinosyl transferase from Mycobacterium smegmatis (1074 aa), FASTA scores: opt: 1870, E(): 5.1e-97, (41.4% identity in 1113 aa overlap); etc. Also similar to P72059|EMBC|Rv3793|MTCY13D12.27 indolylacetylinositol arabinosyltransferase from Mycobacterium tuberculosis (1094 aa), FASTA scores: opt: 1974, E(): 7.7e-103, (40.9% identity in 1110 aa overlap); and P72030|EMBB|Rv3795|MTCY13D12.29 indolylacetylinositol arabinosyltransferase from Mycobacterium tuberculosis (1098 aa), FASTA scores: opt: 1288, E(): 2.1e-64, (42.5% identity in 1114 aa overlap). Supposedly regulated by embR|Rv1267c. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WNL9 No cell wall and cell processes P0A560 P9WNL9 GO:0016021,GO:0009252,GO:0005886,GO:0046677,GO:0016763,GO:0007047 2.4.2.- EMB P9WNL9 NC_000962.3 Mycobrowser_v5 CDS 4251085 4252866 . + 0 Rv3797 fadE35 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE35 Rv3797, (MTV026.02), len: 593 aa. Probable fadE35, acyl-CoA dehydrogenase, similar to many e.g. Q9HY33|PA3593 from Pseudomonas aeruginosa (575 aa) FASTA scores: opt: 838, E(): 2.1e-46, (35.3% identity in 569 aa overlap); Q9ANZ8|AIDB from Burkholderia pseudomallei (Pseudomonas pseudomallei) (554 aa), FASTA scores: opt: 633, E(): 3.4e-33, (33.1% identity in 480 aa overlap); Q9HX44|PA3972 from Pseudomonas aeruginosa (549 aa) FASTA scores: opt: 560, E(): 1.7e-28, (29.9% identity in 569 aa overlap); P33224|AIDB_ECOLI|B4187 from Escherichia coli strain K12 (541 aa), FASTA scores: opt: 455, E(): 1e-21, (31.15% identity in 514 aa overlap); etc. Also similar to O86368|FADE8|Rv0672|MTCI376.02c acyl-CoA dehydrogenase from Mycobacterium tuberculosis (542 aa), FASTA scores: opt: 479, E(): 2.9e-23, (32.2% identity in 460 aa overlap). Could belong to the acyl-CoA dehydrogenases family. O53577 No lipid metabolism O53577 O53577 GO:0055114,GO:0003995 1.3.99.- O53577 NC_000962.3 Mycobrowser_v5 CDS 4254380 4255948 . - 0 Rv3799c accD4 Key enzyme in the catabolic pathway of odd-chain fatty acids, isoleucine, threonine, methionine, and valine [catalytic activity: ATP + propionyl-CoA + CO(2) + H(2)O = ADP + orthophosphate + methylmalonyl-CoA.] Probable propionyl-CoA carboxylase beta chain 4 AccD4 (pccase) (propanoyl-CoA:carbon dioxide ligase) Rv3799c, (MTV026.04c), len: 522 aa. Probable accD4, propionyl-CoA carboxylase beta chain 4, equivalent to Q9CDB0|ACCD4|ML0102 putative acyl CoA carboxylase from Mycobacterium leprae (517 aa) FASTA scores: opt: 3154, E(): 8e-187, (91.2% identity in 511 aa overlap). Also similar to many e.g. Q9X4K7|PCCB from Streptomyces coelicolor (530 aa), FASTA scores: opt: 1714, E(): 4.4e-98, (50.0% identity in 510 aa overlap); P53003|PCCB_SACER from Saccharopolyspora erythraea (Streptomyces erythraeus) (546 aa), FASTA scores: opt: 1549, E(): 6.6e-88, (50.65% identity in 519 aa overlap); Q9WZH5|TM0716 from Thermotoga maritima (515 aa) FASTA scores: opt: 1529, E(): 1.1e-86, (46.7% identity in 512 aa overlap); etc. Also similar to P53002|PCCB_MYCLE|ACCD5|PCCB|ML0731|B1308_C1_125 probable propionyl-CoA carboxylase beta chain 5 from Mycobacterium leprae (549 aa), FASTA scores: opt: 1493, E(): 1.9e-84, (49.8% identity in 514 aa overlap); and P96885|PCC5_MYCTU|ACCD5|PCCB|Rv3280|MT3379.1|MTCY71.20 probable propionyl-CoA carboxylase beta chain 5 from Mycobacterium tuberculosis (548 aa), FASTA scores: opt: 1471, E(): 4.2e-83, (49.15% identity in 515 aa overlap). Belongs to the ACCD/PCCB family. Length extended since first submission (+5 aa). AccA3 (Rv3285), AccD5 (Rv3280), AccD4 (Rv3799), and AccE5 (Rv3281) form a biotin-dependent acyl-CoA carboxylase in M. tuberculosis H37Rv (See Oh et al., 2006). O53578 No lipid metabolism O53578 O53578 GO:0004658 6.4.1.3 O53578 NC_000962.3 Mycobrowser_v5 CDS 4261153 4263066 . - 0 Rv3801c fadD32 Involved in the final steps of mycolic acid biosynthesis. Activates meromycolic acid into meromycoloyl-AMP and transfers the meromycolic acyl chains onto PKS13 Fatty-acid-AMP ligase FadD32 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase). Also shown to have acyl-ACP ligase activity. Rv3801c, (MTV026.06c), len: 637 aa. FadD32, fatty-acid-AMP synthetase, equivalent to Q9CDB2|FADD32|ML0100 putative acyl-CoA synthetase from Mycobacterium leprae (635 aa), FASTA scores: opt: 3892, E(): 0, (93.05% identity in 632 aa overlap); and highly similar to others from Mycobacterium leprae. Also similar to others from Mycobacterium tuberculosis e.g. P95288|FADD31|Rv1925|MTCY09F9.39c (620 aa), FASTA scores: opt: 1567, E(): 1.7e-88, (47.05% identity in 612 aa overlap); MTCY338_18, MTCY349_40, MTV005_21, MTCY24G1_8, MTCY19G5_7, MTCY4D9_17; and MBU75685_1 acyl-CoA ligase from Mycobacterium bovis. O53580 No lipid metabolism O53580 O53580 GO:0008152,GO:0016874 6.2.1.- O53580 NC_000962.3 Mycobrowser_v5 CDS 4268925 4269833 . - 0 Rv3806c ubiA Involved in arabinogalactan synthesis [catalytic activity: phosphoribose diphosphate + decaprenyl phosphate = decaprenylphosphoryl-5-phosphoribose + diphosphate] Decaprenylphosphoryl-5-phosphoribose (DPPR) synthase (decaprenyl-phosphate 5-phosphoribosyltransferase) Rv3806c, (MTV026.11c), len: 302 aa. UbiA, decaprenylphosphoryl-5-phosphoribose (DPPR) synthase (See Huang et al., 2005), equivalent to Q9CDB5|ML0095 putative integral membrane protein from Mycobacterium leprae (302 aa), FASTA scores: opt: 1677, E(): 3.9e-103, (83.75% identity in 302 aa overlap). Also highly similar to others e.g. Q9KZA2|SC5G8.12 putative integral membrane protein from Streptomyces coelicolor (322 aa), FASTA scores: opt: 937, E(): 2e-54, (51.4% identity in 292 aa overlap); AAK79783|CAC1818 conserved membrane protein, possible 4-hydroxybenzoate from Clostridium acetobutylicum (290 aa), FASTA scores: opt: 467, E(): 1.5e-23, (26.9% identity in 290 aa overlap); Q98KY3|MLL1266 nodulation protein NOEC (potential integral membrane protein) from Rhizobium loti (Mesorhizobium loti) (297 aa), FASTA scores: opt: 331, E(): 1.4e-14, (27.4% identity in 299 aa overlap); etc. And highly similar to C-terminal part of Q981F8|MLR9393 nodulation protein NOEC (potential integral membrane protein) from Rhizobium loti (Mesorhizobium loti) plasmid pMLa (541 aa), FASTA scores: opt: 388, E(): 4e-18, (30.9% identity in 301 aa overlap); and P55585|Y4NM_RHISN integral membrane protein (possible permease/transporter) from Rhizobium sp. strain NGR234 plasmid sym pNGR234a (516 aa), FASTA scores: opt: 380, E(): 1.3e-17, (31.85% identity in 295 aa overlap). Contains PS00225 Crystallins beta and gamma 'Greek key' motif signature. P9WFR5 No cell wall and cell processes O53583 P9WFR5 GO:0004659,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 4272276 4273475 . - 0 Rv3809c glf Involved in lipopolysaccharide biosynthesis, in the conversion of UDP-galactopyranose into UDP-galactofuranose through a 2-keto intermediate [catalytic activity: UDP-D-galactopyranose = UDP-D-galacto-1,4-furanose]. UDP-galactopyranose mutase Glf (UDP-GALP mutase) (NAD+-flavin adenine dinucleotide-requiring enzyme) Rv3809c, (MTV026.14), len: 399 aa. Glf (alternate gene name: ceoA), UDP-galactopyranose mutase (see citations below), identical to previously sequenced gene, and equivalent to Q9CDB8|GLF|ML0092 putative UDP-galactopyranose mutase from Mycobacterium leprae (413 aa), FASTA scores: opt: 2347, E(): 1.3e-140, (86.6% identity in 396 aa overlap). Also highly similar to others e.g. AAK61905|EPSJ UDP-galactopyranose mutase (protein involved in exopolysaccharides biosynthesis) from Streptococcus thermophilus (365 aa), FASTA scores: opt: 972, E(): 5.9e-54, (45.85% identity in 375 aa overlap); P37747|GLF_ECOLI|B2036 UDP-galactopyranose mutase from Escherichia coli strain K12 (367 aa), FASTA scores: opt: 958, E(): 4.5e-53, (43.55% identity in 379 aa overlap); O86897|CAP33FN from Streptococcus pneumoniae (369 aa) FASTA scores: opt: 954, E(): 8.1e-53, (44.8% identity in 375 aa overlap); etc. Cofactor: FAD (by similarity). N-terminal SHOWS similarity to FAD or NAD containing proteins. P9WIQ1 No cell wall and cell processes 1V0J O06934 P9WIQ1 GO:0008152,GO:0008767 5.4.99.9 P9WIQ1 NC_000962.3 Mycobrowser_v5 CDS 4274798 4276417 . + 0 Rv3811 Rv3811 Function unknown Conserved hypothetical protein Rv3811, (MTV026.16), len: 539 aa. Conserved hypothetical protein, showing some similarity to Q9KZK5|SCE34.21c putative secreted protein from Streptomyces coelicolor (416 aa), FASTA scores: opt: 603, E(): 8.1e-26, (34.4% identity in 404 aa overlap); Q9S2P9|SC5F7.14c hypothetical 31.9 KDA protein from Streptomyces coelicolor (308 aa), FASTA scores: opt: 472, E(): 9.5e-19, (37.5% identity in 208 aa overlap). Middle section (approximately aa 185-350/390) shows some similarity with Q9GK12 peptidoglycan recognition protein precursor from Camelus dromedarius (Dromedary) (Arabian camel) (193 aa) FASTA scores: opt: 274, E(): 4.6e-08, (32.2% identity in 177 aa overlap); O75594|PGLYRP|PGRP from Homo sapiens (Human) (196 aa), FASTA scores: opt: 272, E(): 6e-08, (30.9% identity in 220 aa overlap); Q9JLN4|PGRP peptidoglycan recognition protein from Rattus norvegicus (Rat) (182 aa), FASTA scores: opt: 253, E(): 6.2e-07, (32.15% identity in 171 aa overlap); etc. C-terminal end shows similarity with Q01377|CSP1_CORGL PS1 protein precursor (one of the two major secreted proteins) from Corynebacterium glutamicum (Brevibacterium flavum) (657 aa), FASTA scores: opt: 250, E(): 2.7e-06, (39.45% identity in 109 aa overlap). Contains PS00687 Aldehydedehydrogenases glutamic acid active site. Note that previously known as csp. Q79F96 No conserved hypotheticals Q79F96 Q79F96 GO:0009253,GO:0005515,GO:0008745 Q79F96 NC_000962.3 Mycobrowser_v5 CDS 4280033 4280788 . - 0 Rv3815c Rv3815c Function unknown; probably involved in cellular metabolism. Possible acyltransferase Rv3815c, (MTCY409.15), len: 251 aa. Possible acyltransferase, highly similar to Q9CDC0|ML0087 putative acyltransferase from Mycobacterium leprae (257 aa), FASTA scores: opt: 845, E(): 2.7e-47, (53.25% identity in 246 aa overlap). Also highly similar to Q9K3R3|2SCG4.01 putative acyltransferase from Streptomyces coelicolor (242 aa), FASTA scores: opt: 656, E(): 3.7e-35, (47.85% identity in 234 aa overlap); and similar to many putative acyltransferases and hypothetical proteins e.g. P74498|SLL1848 hypothetical 24.3 KDA protein from Synechocystis sp. strain PCC 6803 (225 aa) FASTA scores: opt: 275, E(): 1.2e-10, (34.8% identity in 181 aa overlap); Q9ZBS1|SC7A1.02 putative acyltransferase from Streptomyces coelicolor (264 aa), FASTA scores: opt: 266, E(): 5.2e-10, (29.7% identity in 229 aa overlap); Q9PNZ5|AAS|CJ0938 putative 2-acylglycerophosphoethanolamine acyltransferase/ acyl-acyl carrier protein synthetase from Campylobacter jejuni (1170 aa), FASTA scores: opt: 264, E(): 2.3e-09, (23.55% identity in 221 aa overlap) (similarity only with middle section); etc. Also highly similar to upstream ORF O07809|Rv3814c|MTCY409.16 putative acyltransferase from Mycobacterium tuberculosis (261 aa), FASTA scores: opt: 1069, E(): 1e-61, (60.4% identity in 245 aa overlap) ; and downstream ORF O07807|Rv3816c|MTCY409.14 putative acyltransferase from Mycobacterium tuberculosis (259 aa) FASTA scores: opt: 847, E(): 2e-47, (55.7% identity in 246 aa overlap). And similar to O53516|Rv2182c|MTV021.15c hypothetical 27.0 KDA protein from Mycobacterium tuberculosis (247 aa), FASTA scores: opt: 237, E(): 3.6e-08, (30.9% identity in 233 aa overlap). O07808 No intermediary metabolism and respiration O07808 O07808 GO:0008152,GO:0008415 2.3.1.- O07808 NC_000962.3 Mycobrowser_v5 CDS 4280792 4281571 . - 0 Rv3816c Rv3816c Function unknown; probably involved in cellular metabolism. Possible acyltransferase Rv3816c, (MTCY409.14), len: 259 aa. Possible acyltransferase, equivalent to Q9CDC0|ML0087 putative acyltransferase from Mycobacterium leprae (257 aa) FASTA scores: opt: 1401, E(): 1.5e-80, (81.9% identity in 254 aa overlap). Also highly similar to many putative acyltransferases and hypothetical proteins e.g. Q9K3R3|2SCG4.01 putative acyltransferase from Streptomyces coelicolor (242 aa), FASTA scores: opt: 758, E(): 2.4e-40, (51.7% identity in 234 aa overlap); Q9ZBS1|SC7A1.02 putative acyltransferase from Streptomyces coelicolor (264 aa), FASTA scores: opt: 312, E(): 2e-12, (29.55% identity in 237 aa overlap); O67841|AAS|AQ_2058 2-acylglycerophosphoethanolamine acyltransferase from Aquifex aeolicus (211 aa), FASTA scores: opt: 281, E(): 1.5e-10, (32.7% identity in 162 aa overlap); etc. Also highly similar to upstream ORFs O07808|Rv3815c|MTCY409.15 putative acyltransferase from Mycobacterium tuberculosis (251 aa), FASTA scores: opt: 847, E(): 6.7e-46, (55.7% identity in 246 aa overlap); and O07809|Rv3814c|MTCY409.16 putative acyltransferase from Mycobacterium tuberculosis (261 aa), FASTA scores: opt: 776, E(): 1.9e-41, (50.9% identity in 228 aa overlap). O07807 No intermediary metabolism and respiration O07807 O07807 GO:0008152,GO:0008415 2.3.1.- O07807 NC_000962.3 Mycobrowser_v5 CDS 4291639 4293174 . - 0 Rv3824c papA1 Involved in sulfolipid-1 (SL-1) biosynthesis Conserved polyketide synthase associated protein PapA1 Rv3824c, (MTCY409.06), len: 511 aa. papA1, conserved polyketide synthase (PKS) associated protein, highly similar to Q49618|PAPA3|ML1230|B1170_C1_180 PKS-associated protein A3 from Mycobacterium leprae (471 aa), FASTA scores: opt: 1879, E(): 7.1e-111, (55.5% identity in 465 aa overlap). Also similar to Q9F2R3|SCD65.19c hypothetical 52.8 KDA protein from Streptomyces coelicolor (473 aa), FASTA scores: opt: 476, E(): 1.7e-22, (26.7% identity in 464 aa overlap); and similar in part to Q09164|SIMA|CYSYN cyclosporin synthetase from Tolypocladium inflatum (15281 aa) FASTA scores: opt: 238, E(): 2.8e-06, (22.35% identity in 371 aa overlap). Also highly similar to other PKS-associated proteins from Mycobacterium tuberculosis; O50438|PAPA3|Rv1182|MTV005.18 (472 aa), FASTA scores: opt: 1862, E(): 8.4e-110, (55.95% identity in 470 aa overlap); and upstream ORF O07803|PAPA2|Rv3820c|MTCY409.10 (468 aa) FASTA scores: opt: 1664, E(): 2.5e-97, (53.9% identity in 462 aa overlap). Contains PS00453 FKBP-type peptidyl-prolyl cis-trans isomerase signature 1. P9WIK9 No lipid metabolism O07799 P9WIK9 GO:0008415 2.3.1.- NC_000962.3 Mycobrowser_v5 CDS 4301563 4302789 . - 0 Rv3827c Rv3827c Required for the transposition of the insertion sequence IS1537. Possible transposase Rv3827c, (MTCY409.03), len: 408 aa. Possible transposase within IS1537 element, similar to several transposases e.g. O83029|TNPC|DR2324|DR0666|DR0978|DR1381|DR1651|DR1933 transposase from Deinococcus radiodurans(408 aa) FASTA scores: opt: 302, E(): 3.9e-12, (30.75% identity in 358 aa overlap); Q9RXX7|DR0178 putative transposase from Deinococcus radiodurans (409 aa), FASTA scores: opt: 297, E(): 8.2e-12, (31.1% identity in 360 aa overlap); P73816|SLR2062 transposase from Synechocystis sp. strain PCC 6803 (400 aa), FASTA scores: opt: 296, E(): 9.3e-12, (30.05% identity in 353 aa overlap); etc. Highly similar to proteins from Mycobacterium tuberculosis e.g. O33333|Rv2791c|MTV002.56c transposase (459 aa) FASTA scores: opt: 2211, E(): 9.4e-136, (87.75% identity in 367 aa overlap); P95117|Rv2978c|MTCY349.09 hypothetical 51.4 KDA protein (459 aa), FASTA scores: opt: 2165, E(): 9e-133, (85.85% identity in 367 aa overlap); Q10809|YS85_MYCTU|Rv2885c|MT2953|MTCY274.16c hypothetical 51.3 KDA protein (460 aa), FASTA scores: opt: 2127, E(): 2.6e-130, (83.95% identity in 368 aa overlap); O0777|Rv0606|MTCY19H5.16c probable transposase (fragment) (247 aa), FASTA scores: opt: 1405, E(): 9.3e-84, (85.3% identity in 238 aa overlap); etc. O07796 No insertion seqs and phages O07796 O07796 NC_000962.3 Mycobrowser_v5 CDS 4307655 4308914 . - 0 Rv3834c serS Involved in translation mechanism [catalytic activity: ATP + L-serine + tRNA(SER) = AMP + pyrophosphate + L-SERYL-tRNA(SER)]. SERYL-tRNA synthetase SerS (serine--tRNA ligase) (SERRS) (serine translase) Rv3834c, (MTCY01A6.35), len: 419 aa. Probable serS, seryl-tRNA synthetase, equivalent to Q9CDC1|SERS|ML0082 putative SERYL-tRNA synthase from Mycobacterium leprae (417 aa), FASTA scores: opt: 2361, E(): 8.5e-138, (85.8% identity in 416 aa overlap). Also highly similar many e.g. Q9ZBX1|SYS_STRCO|SERS|SCD78.28c from Streptomyces coelicolor (425 aa), FASTA scores: opt: 1594, E(): 1.2e-90, (59.75% identity in 425 aa overlap); Q9X199|SYS_THEMA|SERS|TM1379 from Thermotoga maritima (425 aa), FASTA scores: opt: 1083, E(): 3.3e-59, (43.3% identity in 425 aa overlap); P37464|SYS_BACSU|SERS from Bacillus subtilis (425 aa), FASTA scores: opt: 1015, E(): 5e-55, (39.3% identity in 425 aa overlap); etc. Contains PS00179 Aminoacyl-transfer RNA synthetases class-II signature 1. Belongs to class-II aminoacyl-tRNA synthetase family. P9WFT7 No information pathways P67561 P9WFT7 GO:0005737,GO:0016260,GO:0004828,GO:0006434,GO:0005524 6.1.1.11 P9WFT7 NC_000962.3 Mycobrowser_v5 CDS 4311009 4311707 . - 0 Rv3837c Rv3837c Thought to be involved in glycolisis and perhaps glycogen metabolism [catalytic activity: 3-phosphoglycerate = 2-phosphoglycerate]. Probable phosphoglycerate mutase (phosphoglyceromutase) (phosphoglycerate phosphomutase) Rv3837c, (MTCY01A6.32), len: 232 aa. Probable phosphoglycerate mutase, equivalent to Q9CDC3|ML0079 putative phosphoglycerate mutase from Mycobacterium leprae (231 aa), FASTA scores: opt: 1116, E(): 7.3e-66, (71.55% identity in 232 aa overlap). Also similar to others e.g. Q9ZAX0|PGM 2,3-PDG dependent phosphoglycerate mutase from Amycolatopsis methanolica (205 aa), FASTA scores: opt: 474, E(): 6.4e-24, (41.85% identity in 203 aa overlap); Q9F3Q7|SC10F4.03 putative isomerase from Streptomyces coelicolor (224 aa) FASTA scores: opt: 349, E(): 1e-15, (33.2% identity in 223 aa overlap); Q9RDL0|SCC123.14c putative phosphoglycerate mutase from Streptomyces coelicolor (223 aa), FASTA scores: opt: 256, E(): 1.2e-09, (34.0% identity in 203 aa overlap); Q9RVD2|DR1097 putative phosphoglycerate mutase from Deinococcus radiodurans (232 aa), FASTA scores: opt: 201, E(): 5.1e-06, (31.45% identity in 175 aa overlap); etc. Also similar to P71724|Rv2419c|MTCY428.28|MTCY253.01 hypothetical 24.2 KDA protein from Mycobacterium tuberculosis (223 aa), FASTA scores: opt: 210, E(): 1.3e-06, (32.0% identity in 172 aa overlap). Contains PS00175 Phosphoglycerate mutase family phosphohistidine signature. P96241 No intermediary metabolism and respiration P96241 P96241 GO:0016853 5.4.2.- P96241 NC_000962.3 Mycobrowser_v5 CDS 4313567 4313980 . + 0 Rv3840 Rv3840 Supposedly involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv3840, (MTCY01A6.29c), len: 137 aa. Possible transcriptional regulator, highly similar in part to PSR proteins (penicillin binding protein repressors) e.g. Q47828|PSR PSR protein from Enterococcus hirae (293 aa) FASTA scores: opt: 221, E(): 2.2e-07, (41.65% identity in 108 aa overlap); O86213|PSRFM PSRFM protein (fragment) from Enterococcus hirae (171 aa), FASTA scores: opt: 202, E(): 2.4e-06, (40.75% identity in 108 aa overlap); Q47865|PSR penicillin binding protein repressor from Enterococcus hirae (148 aa), FASTA scores: opt: 201, E(): 2.5e-06, (51.65% identity in 60 aa overlap); etc. Also highly similar in part to other transcriptional regulators e.g. BAB57524|MSRR peptide methionine sulfoxide reductase regulator from Staphylococcus aureus subsp. aureus Mu50 (327 aa), FASTA scores: opt: 195, E(): 1.2e-05, (36.7% identity in 109 aa overlap); Q99Q02|MSRR|SA1195 peptide methionine sulfoxide reductase regulator from Staphylococcus aureus subsp. aureus N315, and Staphylococcus aureus (327 aa), FASTA scores: opt: 192, E(): 1.9e-05, (36.7% identity in 109 aa overlap); Q9K6Q8|LYTR|BH3670 attenuator for lytabc and LYTR expression from Bacillus halodurans (304 aa), FASTA scores: opt: 171, E(): 0.00041, (34.5% identity in 113 aa overlap); etc. P96238 No regulatory proteins P96238 P96238 P96238 NC_000962.3 Mycobrowser_v5 CDS 4326004 4327473 . - 0 Rv3854c ethA Activates the pro-drug ethionamide (ETH); induced ETH sensitivity when overexpressed in Mycobacterium tuberculosis. Monooxygenase EthA Rv3854c, (MTCY01A6.14), len: 489 aa. EthA (alternate gene names: aka, etaA), monooxygenase required for activation of the pro-drug ethionamide (see citations below), highly similar to other monooxygenases e.g. Q9A588|CC2569 monooxygenase (flavin-binding family) from Caulobacter crescentus (498 aa), FASTA scores: opt: 1959, E(): 2.9e-114, (57.6% identity in 481 aa overlap); Q9RZT0|DRB0033 arylesterase/monoxygenase from Deinococcus radiodurans (833 aa), FASTA scores: opt: 1771, E(): 2.2e-102, (53.75% identity in 480 aa overlap); Q9A8K5|CC1348 monooxygenase (flavin-binding family) from Caulobacter crescentus (499 aa), FASTA scores: opt: 1385, E(): 1.4e-78, (43.2% identity in 486 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. O53300|Rv3083|MTV013.04 monoxygenase (495 aa) FASTA scores: opt: 1692, E(): 1.1e-97, (49.7% identity in 489 aa overlap); O53762|Rv0565c|MTV039.03c putative monoxygenase (486 aa), FASTA scores: opt: 1571, E(): 3.7e-90, (49.05% identity in 471 aa overlap); O69708|Rv3741c|MTV025.089c possible oxidoreductase (probably second part of a two component monooxygenase) (224 aa), FASTA scores: opt: 542, E(): 1.7e-26, (50.0% identity in 162 aa overlap); etc. P9WNF9 No intermediary metabolism and respiration P96223 P9WNF9 GO:0050661,GO:0004499,GO:0055114,GO:0050660 1.-.-.- ETH P9WNF9 NC_000962.3 Mycobrowser_v5 CDS 4330039 4331505 . - 0 Rv3858c gltD Probably involved in glutamate biosynthesis [catalytic activity: 2 L-glutamate + NAD(+) = L-glutamine + 2-oxoglutarate + NADH]. Probable NADH-dependent glutamate synthase (small subunit) GltD (L-glutamate synthase) (L-glutamate synthetase) (NADH-glutamate synthase) (glutamate synthase (NADH)) (GLTS beta chain) (NADPH-GOGAT) Rv3858c, (MTCY01A6.10), len: 488 aa. Probable gltD, small subunit of NADH-dependent glutamate synthase, equivalent to Q9CDD4|GLTD|ML0062 NADH-dependent glutamate synthase small subunit from Mycobacterium leprae (488 aa), FASTA scores: opt: 2997, E(): 1e-166, (87.7% identity in 488 aa overlap). Also highly similar to many e.g. Q9S2Z0|SC3A3.03s from Streptomyces coelicolor (487 aa), FASTA scores: opt: 2152, E(): 1.2e-117, (63.85% identity in 487 aa overlap); Q9KPJ3|VC2374 from Vibrio cholerae (489 aa), FASTA scores: opt: 1699, E(): 2.5e-91, (51.75% identity in 487 aa overlap); Q03460|GLSN_MEDSA from Medicago sativa (Alfalfa) (2194 aa), FASTA scores: opt: 1322, E(): 6.2e-69, (54.45% identity in 485 aa overlap); P09832|GLTD_ECOLI from strain (471 aa) FASTA scores: opt: 889, E() : 0, (37.4% identity in 473 aa overlap); etc. Similar to other glutamate synthases. Cofactor: FAD (by similarity). P9WN19 No intermediary metabolism and respiration P96219 P9WN19 GO:0006537,GO:0004355,GO:0051536,GO:0055114,GO:0050660 1.4.1.13 P9WN19 NC_000962.3 Mycobrowser_v5 CDS 4336777 4337949 . + 0 Rv3860 Rv3860 Function unknown Conserved protein Rv3860, (MTCY01A6.08c), len: 390 aa. Conserved protein, showing similarity with hypothetical proteins from Mycobacterium leprae e.g. Q9CDD8|ML0048 (586 aa), FASTA scores: opt: 484, E(): 5.5e-14, (29.95% identity in 407 aa overlap); O33082|MLCB628.11c (478 aa) FASTA scores: opt: 484, E(): 4.8e-14, (29.95% identity in 407 aa overlap); etc. Also some similarity with O86637|SC3C3.03c hypothetical 112.1 KDA protein from Streptomyces coelicolor(1083 aa), FASTA scores: opt: 483, E(): 9.6e-14, (30.45% identity in 404 aa overlap). And some similarity with other proteins from Mycobacterium tuberculosis (strains H37Rv and CDC1551) e.g. O05456|Rv3888c|MTCY15F10.24 hypothetical 37.7 KDA protein (341 aa), FASTA scores: opt: 603, E(): 2.8e-19, (35.2% identity in 284 aa overlap); O06396|Rv0530|MTCY25D10.09 hypothetical 43.0 KDA protein (405 aa), FASTA scores: opt: 538, E(): 2e-16, (31.0% identity in 371 aa overlap); O69740|Rv3876|MTV027.11 (666 aa), FASTA scores: opt: 475, E(): 1.5e-13, (30.2% identity in 391 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). P96217 No conserved hypotheticals P96217 P96217 P96217 NC_000962.3 Mycobrowser_v5 CDS 4340270 4341478 . + 0 Rv3864 espE Function unknown ESX-1 secretion-associated protein EspE Rv3864, (MTCY01A6.04c), len: 402 aa. EspE, ESX-1 secretion-associated protein, similar to Q49722|ML0405|B1620_C2_213|MLCL383.01 hypothetical 40.8 KDA protein from Mycobacterium leprae (394 aa) FASTA scores: opt: 397, E(): 1.2e-12, (31.0% identity in 410 aa overlap). Also similar to various proteins from several organisms e.g. Q9VYF9|CG12723 hypothetical protein from Drosophila melanogaster (Fruit fly) (450 aa), FASTA scores: opt: 291, E(): 2.3e-07, (34.6% identity in 130 aa overlap); Q98UE3 procollagen ALPHA1(III) (fragment) from Xenopus laevis (African clawed frog) (117 aa) FASTA scores: opt: 257, E(): 3.6e-06, (41.75% identity in 103 aa overlap); P27393|CA24_ASCSU collagen alpha 2(IV) chain precursor from Ascaris suum (Pig roundworm) (Ascaris lumbricoides) (1763 aa), FASTA scores: opt: 273, E(): 5.7e-06, (32.1% identity in 240 aa overlap); etc. Also similar to O06267|Rv3616c|MTCY07H7B.06 (392 aa) FASTA scores: opt: 389, E(): 3e-12, (31.6% identity in 399 aa overlap). P9WJD3 No cell wall and cell processes P9WJD3 GO:0005886,GO:0016021 P9WJD3 NC_000962.3 Mycobrowser_v5 CDS 4341880 4342731 . + 0 Rv3866 espG1 Function unknown ESX-1 secretion-associated protein EspG1 Rv3866, (MTCY01A6.01c, MTV027.01), len: 283 aa. espG1, ESX-1 secretion-associated protein. N-terminal end highly similar to O33091|MLCB628.20c hypothetical 13.1 KDA protein from Mycobacterium leprae (122 aa), FASTA scores: opt: 260, E(): 2.1e-09, (43.6% identity in 117 aa overlap); and C-terminal end highly similar to O33090|MLCB628.19c hypothetical 36.7 KDA protein from Mycobacterium leprae (338 aa), FASTA scores: opt: 540, E(): 1.4e-26, (54.5% identity in 156 aa overlap). Also similar to Q9CD34|ML2530 possible DNA-binding protein from Mycobacterium leprae (289 aa), FASTA scores: opt: 146, E(): 0.058, (28.25% identity in 269 aa overlap) and O53694|Rv0289|MTV035.17 hypothetical 31.6 KDA protein from Mycobacterium tuberculosis (295 aa), FASTA scores: opt: 133, E(): 0.39, (28.15% identity in 277 aa overlap). P96210 No cell wall and cell processes P96210 P96210 NC_000962.3 Mycobrowser_v5 CDS 4362032 4363420 . - 0 Rv3882c eccE1 Unknown ESX conserved component EccE1. ESX-1 type VII secretion system protein. Possible membrane protein. Rv3882c, (MTV027.17c, MTCY15F10.30), len: 462 aa. eccE1, esx conserved component, ESX-1 type VII secretion system protein, possible membrane protein, equivalent to O33077|ML0042|MLCB628.05 putative membrane protein from Mycobacterium leprae (467 aa), FASTA scores: opt: 2346, E(): 1.1e-140, (72.1% identity in 462 aa overlap). Also similar to O05459|Rv3885c|MTCY15F10.27 possible membrane protein from Mycobacterium tuberculosis (537 aa) FASTA scores: opt: 283, E(): 2.5e-10, (26.8% identity in 414 aa overlap); and C-terminal end shows similarity with AAK48368|MT4000 hypothetical 45.6 KDA protein from Mycobacterium tuberculosis strain CDC1551 (422 aa) FASTA scores: opt: 215, E(): 4.1e-06, (26.85% identity in 320 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Rv3614c and Rv3882c interact, by yeast two-hybrid analysis (See MacGurn et al., 2005). P9WJE9 No cell wall and cell processes O05462 P9WJE9 GO:0005886,GO:0016021 P9WJE9 NC_000962.3 Mycobrowser_v5 CDS 4345039 4346481 . + 0 Rv3869 eccB1 Unknown ESX conserved component EccB1. ESX-1 type VII secretion system protein. Possible membrane protein. Rv3869, (MTV027.04), len: 480 aa. EccB1, esx conserved component, ESX-1 type VII secretion system protein, possible membrane protein (has hydrophobic stretch near N-terminus), equivalent to O33088|ML0054|MLCB628.17c putative membrane protein from Mycobacterium leprae (481 aa), FASTA scores: opt: 2489, E(): 8.3e-136, (75.75% identity in 478 aa overlap); and similar to others e.g. Q9Z5I3|ML1544|MLCB596.27 conserved membrane protein from Mycobacterium leprae (506 aa), FASTA scores: opt: 739, E(): 3.9e-35, (33.65% identity in 490 aa overlap). Also similar to hypothetical proteins from Mycobacterium tuberculosis e.g. O05449|Rv3895c|MTCY15F10.17 (495 aa), FASTA scores: opt: 795, E(): 2.3e-38, (35.8% identity in 486 aa overlap); O53933|Rv1782|MTV049.04 (506 aa), FASTA scores: opt: 763, E(): 1.6e-36, (34.7% identity in 490 aa overlap); O06317|Rv3450c|MTCY13E12.03c (470 aa) FASTA scores: opt: 717, E(): 6.7e-34, (32.55% identity in 479 aa overlap); etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WNR7 No cell wall and cell processes O69734 P9WNR7 GO:0005886,GO:0016021 P9WNR7 NC_000962.3 Mycobrowser_v5 CDS 4348827 4350602 . + 0 Rv3871 eccCb1 Function unknown ESX conserved component EccCb1. ESX-1 type VII secretion system protein. Rv3871, (MTV027.06), len: 591 aa. EccCb1, esx conserved component, ESX-1 type VII secretion system protein, equivalent to Q9CDD7|ML0052 hypothetical protein from Mycobacterium leprae (597 aa) FASTA scores: opt: 3341, E(): 9.8e-192, (80.85% identity in 596 aa overlap); and O33086|MLCB628.15c hypothetical protein from Mycobacterium leprae (597 aa), FASTA scores: opt: 3329, E(): 5.1e-191, (80.55% identity in 596 aa overlap). And similar to C-terminal end of others e.g. Q9Z5I2|ML1543|MLCB596.28 possible SPOIIIE-family membrane protein from Mycobacterium leprae (1345 aa), FASTA scores: opt: 601, E(): 5.6e-28, (32.3% identity in 613 aa overlap); O86653|SC3C3.20c ATP/GTP binding protein from Streptomyces coelicolor (1321 aa), FASTA scores: opt: 977, E(): 2.1e-50, (35.15% identity in 583 aa overlap); Q9L0T6|SCD35.15c putative cell division-related protein from Streptomyces coelicolor (1525 aa), FASTA scores: opt: 414, E(): 9e-17, (27.6% identity in 424 aa overlap);P71068|YUKA YUKA protein from Bacillus subtilis (1207 aa), FASTA scores: opt: 343, E(): 1.3e-12, (25.8% identity in 395 aa overlap); etc. And similar to to C-terminal end of hypothetical proteins from Mycobacterium tuberculosis e.g. O06264|Rv3447c|MTCY77.19c (1236 aa) FASTA scores: opt: 845, E(): 1.5e-42, (35.3% identity in 586 aa overlap); O53689|Rv0284|MTV035.12 (1330 aa) FASTA scores: opt: 646, E(): 1.2e-30, (33.35% identity in 606 aa overlap); O53935|Rv1784|MTV049.06 (932 aa) FASTA scores: opt: 589, E(): 2.1e-27, (33.1% identity in 619 aa overlap); etc. Contains 2 X PS00017 ATP/GTP-binding site motif A (P-loop). Note some similarity (with hypothetical proteins from Mycobacterium tuberculosis and P71068|YUKA) continues in upstream ORF MTV027.05. P9WNB1 No cell wall and cell processes O69736 P9WNB1 GO:0003677,GO:0007049,GO:0051301,GO:0007059,GO:0005737,GO:0016021,GO:0017111,GO:0005524 P9WNB1 NC_000962.3 Mycobrowser_v5 CDS 4383008 4383640 . - 0 Rv3897c Rv3897c Function unknown Conserved hypothetical protein Rv3897c, (MTCY15F10.15), len: 210 aa. Conserved hypothetical protein, highly similar in part to Q10691|YK83_MYCTU|Rv2083|MT2145|MTCY49.22 hypothetical 30.8 KDA protein from Mycobacterium tuberculosis (314 aa) FASTA scores: opt: 815, E(): 4.7e-26, (73.05% identity in 167 aa overlap). Similarity to MTCY49.22 suggests that this is a continuation of MTCY15F10.14. There is a frameshift mutation near 3'-end with respect to this sequence as well, similarity to MTCY49.22 continues in an overlapping ORF. Sequence appears to be correct. O07036 No conserved hypotheticals O07036 NC_000962.3 Mycobrowser_v5 CDS 4360543 4361925 . - 0 Rv3881c espB Function unknown. Is a proteolytic substrate of MYCP1|Rv3883c. Secreted ESX-1 substrate protein B, EspB. Conserved alanine and glycine rich protein Rv3881c, (MTV027.16c), len: 460 aa. EspB, ESX-1 substrate protein B (See McLaughlin et al., 2007). Conserved ala-, gly-rich protein. C-terminal end highly similar to O06126 hypothetical 9.5 KDA protein (fragment) from Mycobacterium tuberculosis strain NTI 64719 (90 aa) FASTA scores: opt: 333, E(): 6.3e-07, (69.75% identity in 86 aa overlap) but sequence difference causes frameshift in NTI 64719. Also similar to part of small Mycobacterium leprae ORF O33078|MLCB628.06 (EMBL:Y14967) (101 aa), FASTA scores: opt: 194, E(): 0.04, (59.3% identity in 54 aa overlap), suggesting this is represented by a pseudogene in Mycobacterium leprae. P9WJD9 No cell wall and cell processes P9WJD9 GO:0005576 P9WJD9 NC_000962.3 Mycobrowser_v5 CDS 4364979 4366838 . - 0 Rv3884c eccA2 Function unknown ESX conserved component EccA2. ESX-2 type VII secretion system protein. Probable CbxX/CfqX family protein. Rv3884c, (MTCY15F10.28), len: 619 aa. eccA2, esx conserved component, ESX-2 type VII secretion system protein. Probable CbxX/CfqX protein family, similar to hypothetical proteins from Mycobacterium leprae e.g. Q9CD28|Y282_MYCLE|ML2537 (640 aa), FASTA scores: opt: 725, E(): 2.9e-34, (28.95% identity in 587 aa overlap); O33089|Y2G8_MYCLE|ML0055|MLCB628.18c (belongs to the CbxX/CfqX family) (573 aa); Q9CBV5|ML1536 (610 aa) FASTA scores: opt: 648, E(): 7.4e-30, (31.5% identity in 549 aa overlap). Also similar to proteins belonging to the CbxX/CfqX family e.g. Q9RKZ2|SC6D7.05c putative CbxX/CfqX family protein from Streptomyces coelicolor (618 aa) FASTA scores: opt: 557, E(): 1.3e-24, (28.6% identity in 601 aa overlap); P27643|SP5K_BACSU|SPOVK|SPOVJ stage V sporulation protein K from Bacillus subtilis (322 aa) FASTA scores: opt: 485, E(): 1.1e-20, (35.0% identity in 280 aa overlap) (similarity only at C-terminus); Q9KAC6|BH2363 stage V sporulation protein K from Bacillus halodurans (315 aa), FASTA scores: opt: 462, E(): 2.2e-19, (36.05% identity in 244 aa overlap) (similarity only at C-terminus); etc. And similar to hypothetical proteins from Mycobacterium tuberculosis belonging to the CbxX/CfqX family e.g. O53687|Y282_MYCTU|Rv0282|MT0295|MTV035.10 hypothetical 68.1 KDA protein (631 aa), FASTA scores: opt: 743, E(): 2.6e-35, (29.9% identity in 612 aa overlap); O69733|Y2G8_MYCTU|Rv3868|MT3981|MTV027.03 hypothetical 62.4 KDA protein (573 aa), FASTA scores: opt: 678, E(): 1.3e-31, (31.25% identity in 589 aa overlap); O53947|YH98_MYCTU|Rv1798|MT1847|MTV049.20 (610 aa) FASTA scores: opt: 669, E(): 4.6e-31, (30.95% identity in 549 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Seems to belong to the CbxX/CfqX family. P9WPH7 No cell wall and cell processes O05460 P9WPH7 GO:0005737,GO:0017111,GO:0005524 P9WPH7 NC_000962.3 Mycobrowser_v5 CDS 4380453 4381940 . - 0 Rv3895c eccB2 Unknown ESX conserved component EccB2. ESX-2 type VII secretion system protein. Probable membrane protein. Rv3895c, (MTCY15F10.17), len: 495 aa. EccB2, esx conserved component, ESX-2 type VII secretion system protein, probable membrane protein, highly similar to two conserved membrane protein from Mycobacterium leprae: Q9Z5I3|ML1544|MLCB596.27 (506 aa), FASTA scores: opt: 1070, E(): 1.4e-53, (39.8% identity in 485 aa overlap); and Q9CD29|ML2536 (552 aa), FASTA scores: opt: 483, E(): 4e-20, (36.85% identity in 499 aa overlap). Also highly similar to various proteins from Mycobacterium tuberculosis e.g. O53933|Rv1782|MTV049.04 hypothetical protein (506 aa), FASTA scores: opt: 1106, E(): 1.2e-55, (41.25% identity in 485 aa overlap); O69734|Rv3869|MTV027.04 hypothetical protein (480 aa), FASTA scores: opt: 795, E(): 6.1e-38, (36.0% identity in 486 aa overlap); O33088|ML0054|MLCB628.17c putative membrane protein (481 aa), FASTA scores: opt: 740, E(): 8.3e-35, (35.65% identity in 485 aa overlap); etc. P9WNR5 No cell wall and cell processes O05449 P9WNR5 GO:0005886,GO:0016021 P9WNR5 NC_000962.3 Mycobrowser_v5 CDS 4384147 4385379 . - 0 Rv3899c Rv3899c Function unknown Conserved hypothetical protein Rv3899c, (MTCY15F10.13), len: 410 aa. Conserved hypothetical protein, similar in part to proteins from Mycobacterium tuberculosis strains H37Rv and CDC1551. Region between aa 29-80 is strictly identical to P96909 hypothetical 15.1 KDA protein (fragment) (143 aa) FASTA scores: opt: 562, E(): 4e-16, (69.0% identity in 142 aa overlap); and the N-terminal end is highly similar, but longer 65 aa, to O07266 hypothetical 13.7 KDA protein (fragment) (143 aa), FASTA scores: opt: 562, E(): 4e-16, (69.0% identity in 142 aa overlap). Highly similar to C-terminal end of Q10690|YK82_MYCTU|Rv2082|MTCY49.21 hypothetical 73.6 KDA protein (721 aa), FASTA scores: opt: 1388, E(): 1.5e-48, (55.25% identity in 409 aa overlap). And similar to P71599|Rv0029|MTCY10H4.29 hypothetical 39.6 KDA protein (365 aa), FASTA scores: opt: 403, E(): 1.7e-09, (33.75% identity in 252 aa overlap). Note that MTCY15F10.12 and MTCY15F10.13 appear frameshifted with respect to MTCY49.21 although the sequence appears to be correct. O05446 No conserved hypotheticals O05446 O05446 NC_000962.3 Mycobrowser_v5 CDS 4391631 4393073 . - 0 Rv3907c pcnA Involved in transcription mechanism [catalytic activity: N ATP + (nucleotide)(M) = N diphosphate + (nucleotide)(M+N)]. Probable poly(A) polymerase PcnA (polynucleotide adenylyltransferase) (NTP polymerase) (RNA adenylating enzyme) (poly(A) polymerase) Rv3907c, (MTCY15F10.04), len: 480 aa. Probable pcnA, polynucleotide polymerase, equivalent to Q9CCY1|PCNA|ML2697 PCNA protein from Mycobacterium leprae (486 aa), FASTA scores: opt: 2713, E(): 4.3e-160, (84.1% identity in 478 aa overlap); and Q59534|PCNB POLYA polymerase from Mycobacterium leprae (411 aa) FASTA scores: opt: 2077, E(): 7.1e-121, (82.55% identity in 373 aa overlap). Also highly similar to many e.g. Q9X8T2|SCH24.18 putative RNA nucleotidyltransferase from Streptomyces coelicolor (483 aa), FASTA scores: opt: 1856, E(): 3.7e-107, (61.55% identity in 455 aa overlap); Q9ZN65 POLYA polymerase from Prevotella ruminicola (Bacteroides ruminicola) (479 aa), FASTA scores: opt: 830, E(): 8.5e-44, (34.85% identity in 445 aa overlap); P42977|PAPS_BACSU poly(A) polymerase from Bacillus subtilis (397 aa), FASTA scores: opt: 479, E(): 3.5e-22, (29.35% identity in 450 aa overlap); etc. Contains: PS00017 ATP/GTP-binding site motif A (P-loop), PS00018 EF-hand calcium-binding domain, and probably less significant a PS00237 G-protein coupled receptor signature, and PS00639 Eukaryotic thiol (cysteine) proteases histidine active site. Belongs to the tRNA nucleotidyltransferase / poly(A) polymerase family. L7N672 No information pathways Q7D4M2 L7N672 GO:0003723,GO:0006396,GO:0004652,GO:0005524 2.7.7.19 L7N672 NC_000962.3 Mycobrowser_v5 CDS 4394192 4396600 . + 0 Rv3909 Rv3909 Function unknown Conserved protein Rv3909, (MTCY15F10.02c), len: 802 aa. Conserved protein, equivalent to Q9CCY0|ML2699 putative secreted protein from Mycobacterium leprae (797 aa) FASTA scores: opt: 3777, E(): 8.8e-206, (72.35% identity in 803 aa overlap). Note that the N-terminal end is highly similar to Q50196|L222-ORF7 (286 aa), FASTA scores: opt: 1213, E(): 2.7e-61, (71.75% identity in 255 aa overlap); and the C-terminal end is highly similar to Q50197|L222-ORF8 also from Mycobacterium leprae (512 aa) FASTA scores: opt: 2375, E(): 9.9e-127, (71.8% identity in 518 aa overlap). Shows some similarity with N-terminal end of Q9I2M3|PA1874 hypothetical protein from Pseudomonas aeruginosa (2468 aa), FASTA scores: opt: 171, E(): 0.13, (22.9% identity in 672 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). O05436 No conserved hypotheticals O05436 O05436 NC_000962.3 Mycobrowser_v5 CDS 4400186 4400854 . + 0 Rv3911 sigM The sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released. Possible alternative RNA polymerase sigma factor SigM Rv3911, (MTV028.02), len: 222 aa. Possible sigM, alternative RNA polymerase sigma factor (see Gomez et al., 1997; Chen et al., 2000), highly similar to others e.g. Q9S6U3|SCH24.14c (alias O86856|SIGT) putative RNA polymerase sigma factor from Streptomyces coelicolor (236 aa), FASTA scores: opt: 336, E(): 2.8e-13, (41.5% identity in 212 aa overlap); Q98KG8|MLR1481 probable RNA polymerase sigma subunit from Rhizobium loti (Mesorhizobium loti) (307 aa), FASTA scores: opt: 221, E(): 2.9e-06, (32.95% identity in 179 aa overlap); Q9A4S9|CC2751 putative RNA polymerase sigma factor from Caulobacter crescentus (186 aa), FASTA scores: opt: 217, E(): 3.3e-06, (36.95% identity in 138 aa overlap); etc. Also similarity with other mycobacterial factors e.g. O06289|SIGE|Rv1221|MTCI61.04 putative RNA polymerase sigma factor from Mycobacterium tuberculosis (257 aa), FASTA scores: opt: 193, E(): 0.00012, (33.15% identity in 163 aa overlap); and O05735|SIGE putative RNA polymerase sigma factor from Mycobacterium avium (251 aa), FASTA scores: opt: 192, E(): 0.00014, (33.15% identity in 163 aa overlap). Equivalent to AAK48395|MT4030 RNA polymerase sigma-70 factor from Mycobacterium tuberculosis strain CDC1551 (196 aa) but without similarity at the C-terminal end. Belongs to the sigma-70 factor family, ECF subfamily. O53590 No information pathways O53590 O53590 GO:0016987,GO:0003700,GO:0006352,GO:0006355 O53590 NC_000962.3 Mycobrowser_v5 CDS 4403192 4404412 . + 0 Rv3915 Rv3915 Function unknown; probably involved in cellular metabolism. Probable peptidoglycan hydrolase Rv3915, (MTV028.06), len: 406 aa. Probable peptidoglycan hydrolase, equivalent to Q9CCX8|ML2704 putative hydrolase from Mycobacterium leprae (406 aa) FASTA scores: opt: 2341, E(): 2.7e-138, (86.95% identity in 406 aa overlap); the N-terminal end is highly similar to Q59535 N-acetymuramyl-L-alanine amidase from Mycobacterium leprae (205 aa), FASTA scores: opt: 1046, E(): 5.7e-58, (84.85% identity in 185 aa overlap). Also similar to other hydrolases (especially amidases) e.g. C-terminal end of Q9K6R3|LYTC|BH3665 N-acetylmuramoyl-L-alanine amidase (major autolysin) from Bacillus halodurans (588 aa), FASTA scores: opt: 363, E(): 4.3e-15, (33.15% identity in 356 aa overlap); Q9PKC7|TC0539 putative N-acetylmuramoyl-L-alanine amidase from Chlamydia muridarum (268 aa), FASTA scores: opt: 285, E(): 1.6e-10, (26.05% identity in 242 aa overlap) (RV3915 product appears longer 127 aa); Q9S596|PDCA penicillin-resistant DD-carboxypeptidase from Myxococcus xanthus (302 aa), FASTA scores: opt: 270, E(): 1.5e-09, (39.85% identity in 158 aa overlap); etc. Note that previously known as cwlM. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). L7N653 No intermediary metabolism and respiration Q7D4L9 L7N653 GO:0009253,GO:0008745 3.-.-.- L7N653 NC_000962.3 Mycobrowser_v5 CDS 4405457 4406491 . - 0 Rv3917c parB Involved in chromosome partition. Localize to both POLES of the predivisional cell following completion of DNA replication. Binds to the DNA origin of replication. Probable chromosome partitioning protein ParB Rv3917c, (MTV028.08c, MT4036), len: 344 aa. Probable parB, chromosome partitioning protein, equivalent to Q50201|PARB_MYCLE|ML2706 probable chromosome partitioning protein from Mycobacterium leprae (333 aa), FASTA scores: opt: 1654, E(): 1.6e-88, (78.6% identity in 332 aa overlap). Also highly similar to to others e.g. Q9S6U1|STH24.09 putative partitioning or sporulation protein from Streptomyces coelicolor (328 aa), FASTA scores: opt: 966, E(): 9.7e-49, (58.55% identity in 287 aa overlap) (no similarity on N-terminus); Q9PB63|PARB_XYLFA|XF2281 probable chromosome partitioning protein from Xylella fastidiosa (310 aa), FASTA scores: opt: 598, E(): 1.8e-27, (38.65% identity in 326 aa overlap); P31857|PARB_PSEPU probable chromosome partitioning protein from Pseudomonas putida (290 aa), FASTA scores: opt: 573, E(): 4.6e-26, (40.35% identity in 322 aa overlap); etc. Contains probable helix-turn-helix motif at aa 179 to 200 (Score 1150, +3.1 0 SD). Belongs to the ParB family. Note that previously known as parA. P9WIJ9 No cell wall and cell processes P0A5R2 P9WIJ9 GO:0007059,GO:0003677 P9WIJ9 NC_000962.3 Mycobrowser_v5 CDS 4408969 4410069 . - 0 Rv3921c Rv3921c Unknown Probable conserved transmembrane protein Rv3921c, (MTV028.12c), len: 366 aa. Probable conserved transmembrane protein, equivalent to Q9L7M1 hypothetical 39.2 KDA protein from Mycobacterium paratuberculosis (353 aa), FASTA scores: opt: 2001, E(): 8.4e-100, (83.05% identity in 366 aa overlap); Q9CCX6|ML2710 putative conserved membrane protein from Mycobacterium leprae (380 aa), FASTA scores: opt: 1929, E(): 6.2e-96, (77.1% identity in 380 aa overlap); Q50205 CDS 27 on L222 from Mycobacterium leprae (312 aa) FASTA scores: opt: 1770, E(): 1.6e-87, (88.2% identity in 288 aa overlap). Also similar to other e.g. O54569|STH24.05 inner membrane protein. from Streptomyces coelicolor (431 aa), FASTA scores: opt: 412, E(): 6.5e-15, (33.45% identity in 266 aa overlap); O84253|CT251 60 KDA inner membrane protein from Chlamydia trachomatis (787 aa), FASTA scores: opt: 304, E(): 6e-09, (27.9% identity in 269 aa overlap); P29431|60IM_BUCAP 60 KDA inner-membrane protein homolog from Buchnera aphidicola (subsp. Schizaphis graminum) (536 aa), FASTA scores: opt: 282, E(): 6.7e-08, (36.1% identity in 108 aa overlap); etc. P9WIT5 No cell wall and cell processes P65626 P9WIT5 GO:0005886,GO:0051205,GO:0016021 P9WIT5 NC_000962.3 Mycobrowser_v5 CDS 4410412 4410789 . - 0 Rv3923c rnpA RNaseP catalyzes the removal of the 5'-leader sequence from PRE-tRNA to produce the mature 5'terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme [catalytic activity: endonucleolytic cleavage of RNA, removing 5'-extra-nucleotide from tRNA precursor]. Ribonuclease P protein component RnpA (RNaseP protein) (RNase P protein) (protein C5) Rv3923c, (MT4041, MTV028.14c), len: 125 aa. RnpA, ribonuclease P protein component (see citations below), equivalent, but longer ~10 aa, to P46610|RNPA_MYCLE|ML2712 ribonuclease P protein component from Mycobacterium leprae (120 aa), FASTA scores: opt: 456, E(): 3.3e-24, (63.0% identity in 119 aa overlap); and Q9L7L9|RNPA from Mycobacterium paratuberculosis (119 aa), FASTA scores: opt: 426, E(): 3.5e-22, (60.65% identity in 122 aa overlap). Also similar to many e.g. P25817|RNPA_STRBI from Streptomyces bikiniensis (123 aa), FASTA scores: opt: 174, E(): 4.2e-05, (36.8% identity in 125 aa overlap); P25814|RNPA_BACSU from Bacillus subtilis (116 aa) FASTA scores: opt: 168, E(): 0.0001, (26.85% identity in 108 aa overlap); P48206|RNPA_STRCO|STH24.03 from Streptomyces coelicolor (123 aa), FASTA scores: opt: 166, E(): 0.00015, (37.6% identity in 125 aa overlap); etc. Contains PS00648 Bacterial Ribonuclease P protein component signature. Belongs to the RnpA family. P9WGZ3 No information pathways P0A5X8 P9WGZ3 GO:0001682,GO:0000049,GO:0004526 3.1.26.5 P9WGZ3 NC_000962.3 Mycobrowser_v5 CDS 580809 581492 . + 0 Rv0491 regX3 Transcriptional regulatory protein part of the two component regulatory system REGX3/SENX3. Two component sensory transduction protein RegX3 (transcriptional regulatory protein) (probably LuxR-family) Rv0491, (MTCY20G9.17), len: 227 aa. RegX3, response regulator protein (sensory transduction protein) (see citations below), equivalent to O07130|RGX3_MYCBO|REGX3 sensory transduction protein from Mycobacterium bovis BCG (227 aa); AAG09797.1|AF258346_2|AF258346|REGX3 response regulator from Mycobacterium smegmatis (228 aa); equivalent to P54884|RGX3_MYCLE|REGX3 sensory transduction protein from Mycobacterium leprae (198 aa), FASTA scores : E(): 0, (95.4% identity in 197 aa overlap). Also highly similar to other response regulators e.g. AAG43239.1|AF123314_2 |AF123314 putative response regulator from Corynebacterium glutamicum (232 aa). P9WGL9 No regulatory proteins 2OQR Q11156 P9WGL9 GO:0006355,GO:0006350,GO:0000156,GO:0000160,GO:0003677 P9WGL9 NC_000962.3 Mycobrowser_v5 CDS 1204067 1205233 . + 0 Rv1079 metB Involved in methionine biosynthesis: converts O-succinyl-L-homoserine to cystathionine [catalytic activity: O-succinyl-L-homoserine + L-cysteine = cystathionine + succinate (can also use hydrogen sulfide and methanethiol as substrates)]. Cystathionine gamma-synthase MetB (CGS) (O-succinylhomoserine [thiol]-lyase) Rv1079, (MTV017.32), len: 388 aa. metB, cystathionine gamma-synthase (see citation below). P46807|METB_MYCLE cystathionine gamma-synthase from Mycobacterium leprae (388 aa), FASTA scores: opt: 2220, E(): 0, (87.3% identity in 387 aa overlap). Also similar to other Mycobacterium tuberculosis enzymes involved in methionine synthesis e.g. Rv0391 and Rv3340. Contains PS00868 Cys/Met metabolism enzymes pyridoxal-phosphate attachment site. Belongs to the trans-sulfuration enzymes family. P9WGB7 No intermediary metabolism and respiration P66875 P9WGB7 GO:0005737,GO:0009086,GO:0030170,GO:0003962 2.5.1.48 P9WGB7 NC_000962.3 Mycobrowser_v5 CDS 2507146 2507637 . + 0 Rv2234 ptpA Involved in signal transduction (via dephosphorylation). Can dephosphorylated in vitro the phosphotyrosine residue of myelin basic protein (MBP) at pH 7.0 [catalytic activity: protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate]. Phosphotyrosine protein phosphatase PtpA (protein-tyrosine-phosphatase) (PTPase) (LMW phosphatase) Rv2234, (MTCY427.15), len: 163 aa. PtpA (alternate gene name: MPtpA), low molecular weight protein-tyrosine-phosphatase (see citations below), similar to other phosphotyrosine protein phosphatases e.g. P53433|PTPA_STRCO low molecular weight protein-tyrosine phosphatase from Streptomyces coelicolor (164 aa), FASTA scores: opt: 455, E(): 3.3e -25, (49.7% identity in 155 aa overlap); PA1S_HUMAN|P24667 red cell acid phosphatase 1, FASTA score: (37.7% identity in 138 aa overlap); etc. Contains a phosphatase catalytic site domain located in N-terminal part. Activity proven biochemically. Supposed a secreted protein. Substrate of PtkA|Rv2232. P9WIA1 No regulatory proteins 1U2P,1U2Q,1ZOJ P65716 P9WIA1 GO:0004725,GO:0006470 3.1.3.48 P9WIA1 NC_000962.3 Mycobrowser_v5 CDS 4211080 4211784 . - 0 Rv3765c tcrX Sensor part of a two component regulatory system. Probable two component transcriptional regulatory protein TcrX Rv3765c, (MTV025.113c), len: 234 aa. Probable tcrX, response regulator of a two-component regulatory system, highly similar to others e.g. Q9ADN7|2SC10A7.24 putative two component system response regulator from Streptomyces coelicolor (271 aa), FASTA scores: opt: 1111, E(): 4.8e-63, (72.3% identity in 231 aa overlap); Q9F161 response regulator from Corynebacterium glutamicum (Brevibacterium flavum) (232 aa), FASTA scores: opt: 692, E(): 1.2e-36, (46.0% identity in 226 aa overlap); Q9KZU5|SCD84.23c putative two-component systen response regulator from Streptomyces coelicolor (248 aa), FASTA scores: opt: 674, E(): 1.7e-35, (44.05% identity in 236 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. Q50806|Rv1033c|MTCY10G2.16 response regulator homolog (257 aa), FASTA scores: opt: 947, E(): 1e-52, (59.5% identity in 232 aa overlap); P71814|Rv0757|MTCY369.02 PHOP-like protein (247 aa) FASTA scores: opt: 829, E(): 2.8e-45, (54.65% identity in 225 aa overlap); O53894|Rv0981|MTV044.09 (230 aa), FASTA scores: opt: 662, E(): 9e-35, (44.65% identity in 224 aa overlap); and also similar to MTCY31_34; MTCY19H5_20; MTY13628_5; MTCY20G9_17; and to MLCB57_27 from Mycobacterium leprae; and MBY13627_3 from Mycobacterium bovis BCG. Equivalent to AAK48236 from Mycobacterium tuberculosis strain CDC1551 (286 aa) but shorter 52 aa. The N-terminal region is similar to that of other regulatory components of sensory transduction systems. Similar to bacterial regulatory proteins involved in signal transduction. O69730 No regulatory proteins O69730 O69730 GO:0006355,GO:0000156,GO:0000160,GO:0003677 O69730 NC_000962.3 Mycobrowser_v5 misc_RNA 3467967 3468334 . - 0 MTB000042 ssr Involved in degradation of proteins encoded by abnormal messenger RNA. 10Sa RNA ssr, match to EM_BA:MT10SARNA X60301 M.tuberculosis gene for 10Sa RNA. Ends changed since first submission (-239 nt). No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 4216968 4217056 . - 0 MTB000049 serT - tRNA-Ser (GCT) serT, tRNA-Ser, anticodon gct, length = 89 No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 2517032 2517134 . - 0 MTB000073 mcr16 Unknown Putative small regulatory RNA mcr16, putative small regulatory RNA (See DiChiara et al., 2010), ends not mapped, ~100 nt band detected by Northern blot. No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 2719597 2720643 . + 0 Rv2423 Rv2423 Unknown Hypothetical protein Rv2423, (MTCY428.24c), len: 348 aa. Hypothetical unknown protein. P71925 No conserved hypotheticals P71925 P71925 P71925 NC_000962.3 Mycobrowser_v5 ncRNA 1413105 1413227 . - MTB000130 ncRv11264c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1455386 1455461 . + MTB000131 ncRv1298 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1455406 1455461 . + MTB000132 ncRv11298 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1473385 1473503 . + MTB000133 ncRv11315 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1497132 1497220 . + MTB000134 ncRv1329 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1564297 1564499 . + MTB000135 ncRv1389 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1692646 1692731 . + MTB000136 ncRv1501 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1816131 1816235 . + MTB000137 ncRv1617 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 3837297 3837458 . - MTB000154 ncRv13418cA Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 2794176 2794249 . + 0 MTB000032 argW - tRNA-Arg (TCT) argW, tRNA-Arg; anticodon tct, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 2827854 2827926 . + 0 MTB000033 hisT - tRNA-His (GTG) hisT, tRNA-His, anticodon gtg, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 2719083 2719355 . + 0 Rv2422 Rv2422 Unknown Hypothetical protein Rv2422, (MTCY428.25c), len: 90 aa. Hypothetical unknown protein. P71926 No conserved hypotheticals P71926 NC_000962.3 Mycobrowser_v5 CDS 2528980 2529174 . - 0 Rv2255c Rv2255c Unknown Hypothetical protein Rv2255c, (MTV022.05c), len: 64 aa. Hypothetical unknown protein. O53529 No conserved hypotheticals O53529 NC_000962.3 Mycobrowser_v5 tRNA 4216865 4216937 . - 0 MTB000048 argU - tRNA-Arg (ACG) argU, tRNA-Arg, anticodon acg, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 4222581 4222667 . - 0 MTB000050 serU - tRNA-Ser (TGA) serU, tRNA-Ser, anticodon tga, length = 87 No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 4314798 4314891 . + 0 MTB000079 ncrMT3949 Unknown Fragment of putative small regulatory RNA ncrMT3949, fragment of putative small regulatory RNA (See Pelly et al., 2012), cloned from M. tuberculosis CDC1551; supported by RNA-seq in H37Rv (unpublished data). No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 23861 25444 . - 0 Rv0020c fhaA Signal transduction Conserved protein with FHA domain, FhaA Rv0020c, (MTCY10H4.20c), len: 527 aa. FhaA, TB39.8, conserved protein with forkhead-associated domain (IPR000253) at C-terminus, may be involved in signal transduction. Alternative start codon in position 24979 has been suggested (see citation below). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P71590 No regulatory proteins 3PO8,3POA,2LC0,2LC1 P71590 P71590 P71590 NC_000962.3 Mycobrowser_v5 CDS 5240 7267 . + 0 Rv0005 gyrB DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [catalytic activity: ATP-dependent breakage, passage and rejoining of double-stranded DNA]. DNA gyrase (subunit B) GyrB (DNA topoisomerase (ATP-hydrolysing)) (DNA topoisomerase II) (type II DNA topoisomerase) Rv0005, (MTCY10H4.03), len: 675 aa. GyrB, DNA gyrase subunit B (see citations below). Contains PS00177 DNA topoisomerase II signature. Belongs to the type II topoisomerase family. Start changed since first submission (-39 aa). P9WG45 No information pathways 2ZJT,3IG0,3M4I P0C5C5 P9WG45 GO:0003918,GO:0006265,GO:0005524 5.99.1.3 FLQ P9WG45 NC_000962.3 Mycobrowser_v5 CDS 39056 39829 . - 0 Rv0036c Rv0036c Function unknown Conserved protein Rv0036c, (MTCY10H4.36c), len: 257 aa. Conserved protein, highly similar to CAB95889.1|AL359988 conserved hypothetical protein from Streptomyces (276 aa). Also some similarity to Rv3099c|MTCY164_10 (283 aa), FASTA scores: E(): 3.3e-05, (25.9% identity in 205 aa overlap). P9WM91 No conserved hypotheticals P64675 P9WM91 P9WM91 NC_000962.3 Mycobrowser_v5 CDS 165323 165826 . + 0 Rv0138 Rv0138 Function unknown Conserved hypothetical protein Rv0138, (MTCI5.12), len: 167 aa. Conserved hypothetical protein, showing weak similarity to Q10827|YT10_MYCTU hypothetical 17.0 KDA protein from Mycobacterium tuberculosis (147 aa), FASTA scores: opt: 131, E(): 0.047, (31.15% identity in 106 aa overlap). P96815 No conserved hypotheticals P96815 P96815 NC_000962.3 Mycobrowser_v5 CDS 2386293 2387171 . + 0 Rv2125 Rv2125 Unknown Conserved hypothetical protein Rv2125, (MTCY261.21), len: 292 aa. Conserved hypothetical protein, similar to many. O33260 No conserved hypotheticals O33260 O33260 O33260 NC_000962.3 Mycobrowser_v5 CDS 4140239 4140463 . - 0 Rv3697A vapB48 Unknown Possible antitoxin VapB48 Rv3697A, len: 74 aa. Possible vapB48, antitoxin, part of toxin-antitoxin (TA) operon with Rv3697c, see Arcus et al. 2005. Similar to others in M. tuberculosis e.g. Rv3321c, Rv0748 P9WJ15 No virulence, detoxification, adaptation P9WJ15 P9WJ15 NC_000962.3 Mycobrowser_v5 CDS 4305757 4306239 . + 0 Rv3831 Rv3831 Unknown Hypothetical protein Rv3831, (MTCY01A6.38c), len: 160 aa. Hypothetical unknown protein. P96247 No conserved hypotheticals P96247 P96247 NC_000962.3 Mycobrowser_v5 CDS 4324683 4324967 . + 0 Rv3851 Rv3851 Unknown Possible membrane protein Rv3851, (MTCY01A6.17c), len: 94 aa. Possible membrane protein. P96226 No cell wall and cell processes P96226 P96226 NC_000962.3 Mycobrowser_v5 tRNA 25644 25726 . + 0 MTB000003 leuT - tRNA-Leu (CAG) leuT, tRNA-Leu, anticodon cag, length = 83 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 14914 15612 . + 0 Rv0013 trpG Possibly involved in biosynthesis of tryptophan (at the first step). Supposed tetramer of two components I and two components II: component I (Rv1609|trpE) catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II (Rv0013|trpG) provides glutamine amidotransferase activity. Possibly participates in the tryptophan-dependent indole-3-acetic acid production [catalytic activity: chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate]. Possible anthranilate synthase component II TrpG (glutamine amidotransferase) Rv0013, (MTCY10H4.13), len: 232 aa. Possible trpG, anthranilate synthase component II (glutamine amidotransferase). Contains PS00606 Beta-ketoacyl synthases active site; and PS00442 Glutamine amidotransferases class-I active site. Similarity to other type-1 glutamine amidotransferase domains. Note that previously known as pabA. P9WN35 No intermediary metabolism and respiration Q7DAK6 P9WN35 GO:0006541,GO:0000162,GO:0004049 4.1.3.27 P9WN35 NC_000962.3 Mycobrowser_v5 CDS 17467 18762 . - 0 Rv0015c pknA Involved in signal transduction (via phosphorylation). Thought to regulate morphological changes associated with cell division/differentiation process. Phosphorylates at serine and threonine residues [catalytic activity: ATP + a protein = ADP + a phosphoprotein]. Transmembrane serine/threonine-protein kinase A PknA (protein kinase A) (STPK A) Rv0015c, (MTCY10H4.15c), len: 431 aa. PknA, transmembrane serine/threonine-protein kinase, magnesium/manganese dependent (see citations below). Contains PS00108 Serine/Threonine protein kinases active-site signature. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases. It has been shown that sodium orthovanadate inhibits the activity of the enzyme in vitro. P9WI83 No regulatory proteins P65726 P9WI83 GO:0006468,GO:0004674,GO:0005524 2.7.11.1 P9WI83 NC_000962.3 Mycobrowser_v5 CDS 2322552 2323178 . + 0 Rv2065 cobH Required for cobalamin biosynthesis. Precorrin-8X methylmutase CobH (aka precorrin isomerase) Rv2065, (MTCY49.04), len: 208 aa. CobH, precorrin-8X methylmutase (aka precorrin isomerase), similar to many. P9WP87 No intermediary metabolism and respiration P63839 P9WP87 GO:0016993,GO:0009236 5.4.1.2 P9WP87 NC_000962.3 Mycobrowser_v5 CDS 20231 21640 . - 0 Rv0017c rodA This is a septum-peptidoglycan biosynthetic protein, involved in cell wall formation. Plays a role in the stabilization of the FTSZ ring during cell division. Probable cell division protein RodA Rv0017c, (MTCY10H4.17c), len: 469 aa. Probable rodA (alternate gene name: ftsW), cell division protein, integral membrane protein. Belongs to the FTSW/RODA/SPOVE family. P9WN99 No cell wall and cell processes P63760 P9WN99 GO:0051301,GO:0007047,GO:0016021,GO:0009252,GO:0005886,GO:0008360,GO:0007049 NC_000962.3 Mycobrowser_v5 CDS 23270 23737 . - 0 Rv0019c fhaB Signal transduction Conserved protein with FHA domain, FhaB Rv0019c, (MTCY10H4.19c), len: 155 aa. FhaB, conserved protein with forkhead-associated domain (IPR000253), probably involved in signal transduction. P9WJB5 No regulatory proteins P71589 P9WJB5 P9WJB5 NC_000962.3 Mycobrowser_v5 CDS 27023 27442 . - 0 Rv0022c whiB5 Involved in transcriptional mechanism. Probable transcriptional regulatory protein WhiB-like WhiB5 Rv0022c, (MTCY10H4.22c), len: 139 aa. Probable whiB5 (alternate gene name: whmG), WhiB-like regulatory protein (see citations below), similar to WhiB paralogue of Streptomyces coelicolor, wblE gene product (85 aa). P71592 No regulatory proteins P71592 P71592 P71592 NC_000962.3 Mycobrowser_v5 CDS 37259 38947 . + 0 Rv0035 fadD34 Function unknown, but involved in lipid degradation. Probable fatty-acid-CoA ligase FadD34 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv0035, (MTCY10H4.35), len: 562 aa. Probable fadD34, fatty-acid-CoA synthetase, similar to many. L7N699 No lipid metabolism Q7DAJ8 L7N699 GO:0008152,GO:0016874 6.2.1.- L7N699 NC_000962.3 Mycobrowser_v5 CDS 59122 59376 . + 0 Rv0055 rpsR1 This protein has been implicated in aminoacyl-transfer RNA binding. It appears to be situated at the decoding site of messenger RNA. 30S ribosomal protein S18-1 RpsR1 Rv0055, (MTCY21D4.18), len: 84 aa. rpsR1, 30S ribosomal protein S18-1. Belongs to the S18P family of ribosomal proteins. Note that previously known as rpsR. P9WH49 No information pathways P69230 P9WH49 GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WH49 NC_000962.3 Mycobrowser_v5 CDS 1175723 1176112 . + 0 Rv1052 Rv1052 Unknown Hypothetical protein Rv1052, (MTV017.05), len: 129 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53400 No conserved hypotheticals O53400 NC_000962.3 Mycobrowser_v5 CDS 3051619 3051792 . + 0 Rv2737A Rv2737A Function unknown Conserved hypothetical cysteine rich protein (fragment) Rv2737A, len: 57 aa. Conserved hypothetical cys-rich protein (possibly gene fragment), similar to central part of AJ243803_1|glgA from Streptomyces coelicolor glgA (181 aa), FASTA scores: opt: 210, E(): 6.1e-09, (59.25% identity in 54 aa overlap). Q79FB4 No conserved hypotheticals Q79FB4 NC_000962.3 Mycobrowser_v5 CDS 46581 47207 . - 0 Rv0042c Rv0042c Possibly involved in transcriptional mechanism. Possible transcriptional regulatory protein (probably MarR-family) Rv0042c, (MTCY21D4.05c), len: 208 aa. Possible transcriptional regulatory protein, MarR-family. Some similarity to Mycobacterium tuberculosis proteins Rv2327, Rv0880, and Rv1404. P71699 No regulatory proteins P71699 P71699 GO:0006355,GO:0006350,GO:0003700,GO:0005622 P71699 NC_000962.3 Mycobrowser_v5 CDS 47366 48100 . - 0 Rv0043c Rv0043c Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably GntR-family) Rv0043c, (MTCY21D4.06c), len: 244 aa. Probable transcriptional regulator, GntR family, similar to others. P9WMG9 No regulatory proteins P67737 P9WMG9 GO:0006355,GO:0006350,GO:0003700,GO:0005622 P9WMG9 NC_000962.3 Mycobrowser_v5 CDS 48233 49027 . - 0 Rv0044c Rv0044c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0044c, (MTCY21D4.07c), len: 264 aa. Possible oxidoreductase, highly similar to AAD32732.1|MmcI|AF127374| F420-dependent H4MPT reductase from Streptomyces lavendulae (264 aa). Also similar to Mycobacterium tuberculosis proteins e.g. Rv1855c, Rv0953c, Rv0791c, Rv0132c, etc. P71701 No intermediary metabolism and respiration P71701 P71701 GO:0016491,GO:0055114 1.-.-.- P71701 NC_000962.3 Mycobrowser_v5 CDS 97758 98351 . + 0 Rv0089 Rv0089 Thought to cause methylation. Possible methyltransferase/methylase Rv0089, (MTCY251.07), len: 197 aa. Possible methyltransferase, showing some weak similarity to others. Also some similarity with many biotin biosynthesis proteins. Belongs to the methyltransferase superfamily. P9WK03 No intermediary metabolism and respiration P65346 P9WK03 GO:0008168,GO:0008152 2.1.1.- P9WK03 NC_000962.3 Mycobrowser_v5 CDS 49043 49939 . - 0 Rv0045c Rv0045c Function unknown; probably involved in lipid biosynthesis. Possible hydrolase Rv0045c, (MTCY21D4.08c), len: 298 aa. Possible hydrolase, showing similarity with others. Also similar to Mycobacterium tuberculosis proteins Rv3473c, Rv1123c, Rv1938, Rv3617, Rv3670, etc. I6XU97 No lipid metabolism 3P2M P71702 I6XU97 GO:0016787 3.-.-.- I6XU97 NC_000962.3 Mycobrowser_v5 CDS 50021 51124 . - 0 Rv0046c ino1 Involved in phosphatidylinositol (PI) biosynthetic pathway [catalytic activity: D-glucose 6-phosphate = 1L-myo-inositol 1-phosphate]. myo-inositol-1-phosphate synthase Ino1 (inositol 1-phosphate synthetase) (D-glucose 6-phosphate cycloaldolase) (glucose 6-phosphate cyclase) (glucocycloaldolase) Rv0046c, (MTCY21D4.09c), len: 367 aa. Ino1 (alternate gene name: tbINO), myo-inositol-1-phosphate synthase (see citations below). P9WKI1 No intermediary metabolism and respiration 1GR0 P71703 P9WKI1 GO:0006021,GO:0004512,GO:0008654 P9WKI1 NC_000962.3 Mycobrowser_v5 CDS 349624 349932 . + 0 Rv0285 PE5 Function unknown PE family protein PE5 Rv0285, (MTV035.13), len: 102 aa. PE5, Member of the Mycobacterium tuberculosis PE family (see Brennan & Delogu 2002), similar to others e.g. AL0212|MTV012_37 from Mycobacterium tuberculosis (105 aa), FASTA scores: opt: 497, E(): 2.6e-24, (80.4% identity in 102 aa overlap); Z80108|MTCY21B4.03 from Mycobacterium tuberculosis (102 aa), FASTA scores: opt: 413, E(): 3.7e-19, (66.7% identity in 102 aa overlap); etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). L7N695 No PE/PPE Q7DA36 L7N695 L7N695 NC_000962.3 Mycobrowser_v5 CDS 55696 57378 . + 0 Rv0051 Rv0051 Unknown Probable conserved transmembrane protein Rv0051, (MTCY21D4.14), len:560 aa. Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). Probable conserved transmembrane protein. P71708 No cell wall and cell processes P71708 P71708 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 57410 57973 . + 0 Rv0052 Rv0052 Function unknown Conserved protein Rv0052, (MTCY21D4.15), len: 187 aa. Conserved protein, similar to others including Rv1930c from Mycobacterium tuberculosis (174 aa). May be a membrane protein. I6Y6S3 No conserved hypotheticals P71709 I6Y6S3 I6Y6S3 NC_000962.3 Mycobrowser_v5 CDS 58586 59080 . + 0 Rv0054 ssb This protein is essential for replication of the chromosome. It is also involved in DNA recombination and repair. Single-strand binding protein Ssb (helix-destabilizing protein) Rv0054, (MTCY21D4.17), len: 164 aa. ssb, single-strand binding protein (see Mizrahi & Andersen 1998), highly similar to others. Belongs to the SSB family. P9WGD5 No information pathways 1UE1,1UE5,1UE6,1UE7 P0A610 P9WGD5 GO:0006260,GO:0003697,GO:0006281 P9WGD5 NC_000962.3 Mycobrowser_v5 CDS 59409 59867 . + 0 Rv0056 rplI Binds to the 23S rRNA. 50S ribosomal protein L9 RplI Rv0056, (MTCY21D4.19), len: 152 aa. rplI, 50S ribosomal protein L9. Contains PS00651 Ribosomal protein L9 signature. Belongs to the L9P family of ribosomal proteins. P9WH79 No information pathways P66315 P9WH79 GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WH79 NC_000962.3 Mycobrowser_v5 CDS 59896 60417 . + 0 Rv0057 Rv0057 Unknown Hypothetical protein Rv0057, (MTCY21D4.20), len: 173 aa. Hypothetical unknown protein. P9WM77 No conserved hypotheticals P9WM77 NC_000962.3 Mycobrowser_v5 CDS 60396 63020 . + 0 Rv0058 dnaB Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity. The intein is an endonuclease (potential). Probable replicative DNA helicase DnaB Rv0058, (MTV030.01, MTCY21D4.21), len: 874 aa. Probable dnaB, replicative DNA helicase. Contains an intein (position 61630..62838) similar to, and in the same position as, those in Sycnechocystis and Rhodothermus marinus (see citation below) and C-terminal extein (position 62839..63015) similar to many dnaB proteins. This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation. Belongs to the helicase family, DNAB subfamily. In the intein section; belongs to the homing endonuclease family. P9WMR3 No information pathways 2R5U P71715 P9WMR3 GO:0003677,GO:0003678,GO:0006269,GO:0005658,GO:0004519,GO:0016539,GO:0006314,GO:0005524 3.1.-.-,3.6.4.12 P9WMR3 NC_000962.3 Mycobrowser_v5 CDS 63200 63892 . + 0 Rv0059 Rv0059 Unknown Hypothetical protein Rv0059, (MTV030.02), len: 230 aa. Hypothetical unknown protein. O53604 No conserved hypotheticals O53604 NC_000962.3 Mycobrowser_v5 CDS 74629 75198 . - 0 Rv0067c Rv0067c Possibly involved in transcriptional mechanism. Possible transcriptional regulatory protein (possibly TetR-family) Rv0067c, (MTV030.10c), len: 189 aa. Possible transcriptional regulator, highly similar to many. Contains probable helix-turn-helix motif from aa 34 to 55 (Score 1523, +4.37 SD). O53612 No regulatory proteins O53612 O53612 GO:0006350,GO:0003700,GO:0006355 O53612 NC_000962.3 Mycobrowser_v5 rRNA 1473658 1476795 . + 0 MTB000020 rrl - Ribosomal RNA 23S rrl, 23S rRNA gene (approximate coordinates). No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 66923 68362 . + 0 Rv0063 Rv0063 Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0063, (MTV030.06), len: 479 aa. Possible oxidoreductase, similar to many. Similar to Mycobacterium tuberculosis proteins e.g. Rv3107c, Rv1257c, etc. Contains PS00862 Oxygen oxidoreductases covalent FAD-binding site. O53608 No intermediary metabolism and respiration O53608 O53608 GO:0055114,GO:0016491,GO:0050660 1.-.-.- O53608 NC_000962.3 Mycobrowser_v5 CDS 68620 71559 . + 0 Rv0064 Rv0064 Unknown Probable conserved transmembrane protein Rv0064, (MTV030.07), len: 979 aa. Probable conserved transmembrane protein, similar to many. Contains probable coiled-coil domain from aa 948 to 976. P9WFL5 No cell wall and cell processes O53609 P9WFL5 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 71589 71828 . + 0 Rv0064A vapB1 Unknown Possible antitoxin VapB1 Rv0064A, len: 79 aa. Possible vapB1, antitoxin, part of toxin-antitoxin (TA) operon with Rv0065 (See Arcus et al., 2005; Pandey and Gerdes, 2005). Weakly similar to others in Mycobacterium tuberculosis e.g. Rv0300 (73 aa), Rv1721c (75 aa) P0CW29 No virulence, detoxification, adaptation P0CW29 NC_000962.3 Mycobrowser_v5 CDS 71821 72222 . + 0 Rv0065 vapC1 Unknown Possible toxin VapC1 Rv0065, (MTV030.08), len: 133 aa. Possible vapC1, toxin, part of toxin-antitoxin (TA) operon with Rv0064A, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to several others in Mycobacterium tuberculosis: Rv0960 (127 aa), Rv1720c (129 aa), and Rv0549c (137 aa). P9WFC1 No virulence, detoxification, adaptation P9WFC1 P9WFC1 NC_000962.3 Mycobrowser_v5 CDS 89575 89919 . + 0 Rv0081 Rv0081 Involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv0081, (MTV030.25), len: 114 aa. Probable transcriptional regulator, highly similar to others. P9WMI7 No regulatory proteins O53626 P9WMI7 GO:0006355,GO:0006350,GO:0003700,GO:0005622 P9WMI7 NC_000962.3 Mycobrowser_v5 CDS 89924 90403 . + 0 Rv0082 Rv0082 Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv0082, (MTV030.26), len: 159 aa. Probable oxidoreductase, highly similar or similar to other various oxidoreductases. Nucleotide position 90144 in the genome sequence has been corrected, A:G resulting in Q74R. I6XUD2 No intermediary metabolism and respiration O53627 I6XUD2 GO:0008137,GO:0055114,GO:0048038,GO:0051539 1.-.-.- I6XUD2 NC_000962.3 Mycobrowser_v5 CDS 2073943 2074437 . + 0 Rv1829 Rv1829 Function unknown Conserved protein Rv1829, (MTCY1A11.14c), len: 164 aa. Conserved protein, equivalent to O32917|MLCB1788.34|AL008609 Hypothetical protein from Mycobacterium leprae (164 aa), FASTA scores: opt: 1011, E(): 0, (95.1% identity in 164 aa overlap). Also present in Aquifex aeolicus, etc. P9WLR5 No conserved hypotheticals Q50604 P9WLR5 P9WLR5 NC_000962.3 Mycobrowser_v5 CDS 77619 78896 . - 0 Rv0070c glyA2 Key enzyme in the biosynthesis of purines, lipids, other components. Interconversion of serine and glycine [catalytic activity: 5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine]. Serine hydroxymethyltransferase GlyA2 (serine methylase 2) (SHMT 2) Rv0070c, (MTV030.13c), len: 425 aa. glyA2, serine hydroxymethyltransferase. Contains PS00096 Serine hydroxymethyltransferase pyridoxal-phosphate attachment site. Belongs to the ShmT family. Cofactor: pyridoxal phosphate. P9WGI7 No intermediary metabolism and respiration O53615 P9WGI7 GO:0004372,GO:0006544,GO:0006730,GO:0030170,GO:0006563 2.1.2.1 P9WGI7 NC_000962.3 Mycobrowser_v5 CDS 79486 80193 . + 0 Rv0071 Rv0071 Unknown Possible maturase Rv0071, (MTV030.14), len: 235 aa. Possible maturase, similar to many proteins of the group II intron maturase family. Contains 5 VDP repeats at N-terminus, these are also found in two Streptococcus plasmid hypothetical proteins Q52246|X17092 and Q54942|X66468. O53616 No information pathways O53616 O53616 GO:0006278,GO:0003964,GO:0003723 O53616 NC_000962.3 Mycobrowser_v5 CDS 80624 81673 . + 0 Rv0072 Rv0072 Thought to be involved in active transport of glutamine across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable glutamine-transport transmembrane protein ABC transporter Rv0072, (MTV030.16), len: 349 aa. Probable glutamine-transport transmembrane protein ABC-transporter (see citation below). Note that supposed act with near ORF Rv0073|MTV030.17 ATP-binding protein ABC-transporter. P9WG17 No cell wall and cell processes O53617 P9WG17 GO:0005886,GO:0016021 P9WG17 NC_000962.3 Mycobrowser_v5 CDS 82748 83983 . + 0 Rv0074 Rv0074 Function unknown Conserved protein Rv0074, (MTV030.18), len: 411 aa. Conserved protein, similar to many. O53619 No conserved hypotheticals O53619 O53619 GO:0016810 O53619 NC_000962.3 Mycobrowser_v5 CDS 83996 85168 . + 0 Rv0075 Rv0075 Function unknown; probably involved in cellular metabolism. Probable aminotransferase Rv0075, (MTV030.19), len: 390 aa. Probable aminotransferase, similar to many class-II pyridoxal-phosphate-dependent aminotransferases (MALY/PATB subfamily). Also similar to other proteins from Mycobacterium tuberculosis e.g. Rv2294, Rv0858c, etc. O53620 No intermediary metabolism and respiration O53620 O53620 GO:0030170,GO:0008483,GO:0009058 2.6.1.- O53620 NC_000962.3 Mycobrowser_v5 CDS 85183 85572 . - 0 Rv0076c Rv0076c Unknown Probable membrane protein Rv0076c, (MTV030.20c), len: 129 aa. Probable membrane protein, with membrane-spanning domain at C-terminus. O53621 No cell wall and cell processes O53621 NC_000962.3 Mycobrowser_v5 CDS 167271 167681 . - 0 Rv0141c Rv0141c Unknown Unknown protein Rv0141c, (MTCI5.15c), len: 136 aa. Unknown protein. P96818 No conserved hypotheticals P96818 P96818 NC_000962.3 Mycobrowser_v5 CDS 608059 608535 . - 0 Rv0516c Rv0516c May be involved in regulating sigma factor Possible anti-anti-sigma factor Rv0516c, (MTCY20G10.06c), len: 158 aa. Possible anti-anti-sigma factor, showing some similarity to Rv1365c|MTCY02B10_29 from Mycobacterium tuberculosis (128 aa), FASTA scores: E(): 0.0012, (27.4% identity in 124 aa overlap). O33361 No information pathways O33361 O33361 O33361 NC_000962.3 Mycobrowser_v5 CDS 92328 93278 . + 0 Rv0084 hycD Involved in hydrogen metabolism; FHL pathway. Possible formate hydrogenlyase HycD (FHL) Rv0084, (MTCY251.02), len: 316 aa. Possible hycD (alternate gene name: hevD), formate hydrogenlyase, integral membrane protein, similar to others. Belongs to the complex I subunit 1 family. Q10881 No intermediary metabolism and respiration Q10881 Q10881 GO:0016829,GO:0055114,GO:0016491,GO:0016021 1.-.-.- Q10881 NC_000962.3 Mycobrowser_v5 CDS 93289 93951 . + 0 Rv0085 hycP Involved in hydrogen metabolism. Possible hydrogenase HycP Rv0085, (MTCY251.03), len: 220 aa. Possible hycP, hydrogenase, integral membrane protein. Belongs to NADH-ubiquinone/plastoquinone oxidoreductase chain 4L superfamily. P9WM75 No intermediary metabolism and respiration P9WM75 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 93951 95417 . + 0 Rv0086 hycQ Possibly involved in hydrogen metabolism. Possible hydrogenase HycQ Rv0086, (MTCY251.04), len: 488 aa. Possible hycQ, hydrogenase, integral membrane protein. Belongs to the NADH-Ubiquinone/plastoquinone (complex I) superfamily. Q10883 No intermediary metabolism and respiration Q10883 Q10883 GO:0008137,GO:0016021,GO:0042773 1.-.-.- NC_000962.3 Mycobrowser_v5 CDS 95414 96892 . + 0 Rv0087 hycE Involved in hydrogen metabolism; FHL pathway. Possible formate hydrogenase HycE (FHL) Rv0087, (MTCY251.05), len: 492 aa. Possible hycE (alternate gene name: hevE), formate hydrogenlyase, similar to others. Belongs to the complex I 49 kDa subunit family. Q10884 No intermediary metabolism and respiration Q10884 Q10884 GO:0008137,GO:0008901,GO:0016151,GO:0055114,GO:0048038,GO:0051287 1.-.-.- Q10884 NC_000962.3 Mycobrowser_v5 CDS 96927 97601 . + 0 Rv0088 Rv0088 Unknown Possible polyketide cyclase/dehydrase Rv0088, (MTCY251.06), len: 224 aa. Possible polyketide cyclase/dehydrase. Belongs to the SRPBCC ligand-binding domain superfamily. Predicted to be an outer membrane protein (See Song et al., 2008). P9WM73 No lipid metabolism P0A5C3 P9WM73 P9WM73 NC_000962.3 Mycobrowser_v5 CDS 329705 330325 . - 0 Rv0273c Rv0273c Could be involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0273c, (MTV035.01c), len: 206 aa (start uncertain). Possible transcriptional regulator, showing some similarity to hypothetical regulators from Mycobacterium tuberculosis e.g. P96222|Rv3855|MTCY01A6.13c (216 aa); O08377|Rv1534|MTCY07A7A.03 (225 aa), FASTA scores: opt: 123, E(): 3.2e-06, (28.5% identity in 172 aa overlap). O86342 No regulatory proteins O86342 O86342 GO:0006350,GO:0003700,GO:0006355 O86342 NC_000962.3 Mycobrowser_v5 CDS 1183508 1183981 . + 0 Rv1060 Rv1060 Unknown Unknown protein Rv1060, (MTV017.13), len: 157 aa. Unknown protein. O53408 No conserved hypotheticals O53408 O53408 O53408 NC_000962.3 Mycobrowser_v5 CDS 102815 103663 . - 0 Rv0093c Rv0093c Unknown Probable conserved membrane protein Rv0093c, (MTCY251.12c), len: 282 aa. Probable conserved membrane protein, equivalent only to CAC30943.1|AL583924 probable integral membrane protein from Mycobacterium leprae (237 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WM69 No cell wall and cell processes P9WM69 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 103710 104663 . - 0 Rv0094c Rv0094c Function unknown Conserved hypothetical protein Rv0094c, (MTCY251.13c), len: 317 aa. Member of 13E12 repeat family, showing some similarity to U15187|MLU15187_7 from Mycobacterium leprae (94 aa), FASTA score: (49.4% identity in 79 aa overlap). Q50655 No insertion seqs and phages Q50655 Q50655 NC_000962.3 Mycobrowser_v5 CDS 104805 105215 . - 0 Rv0095c Rv0095c Function unknown Conserved hypothetical protein Rv0095c, (MTCY251.14c), len: 136 aa. Member of 13E12 repeat, also partially similar to AF0418|AF041819_8 from Mycobacterium bovis BCG (222 aa), FASTA score: (89.6% identity in 96 aa overlap). Q10891 No insertion seqs and phages Q10891 NC_000962.3 Mycobrowser_v5 CDS 106734 107603 . + 0 Rv0097 Rv0097 Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0097, (MTCY251.16), len: 289 aa. Possible oxidoreductase, equivalent to NP_302343.1|NC_002677 putative oxidoreductase from Mycobacterium leprae (289 aa). Also highly similar to BAB69377.1|AB070955 putative oxidoreductase from Streptomyces avermitilis (296 aa). Contains PS00077 Cytochrome c oxidase subunit I, copper B binding region signature. P9WG83 No intermediary metabolism and respiration P67755 P9WG83 GO:0055114,GO:0016702,GO:0046872 1.-.-.- P9WG83 NC_000962.3 Mycobrowser_v5 CDS 107600 108151 . + 0 Rv0098 fcoT Involved in fatty acid metabolism Probable fatty acyl CoA thioesterase type III FcoT Rv0098, (MTCY251.17), len: 183 aa. FcoT, long-chain fatty acyl CoA thioesterase type III (See Wang et al., 2007), equivalent to CAC30948.1|AL583924 from Mycobacterium leprae (183 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WM67 No lipid metabolism 2PFC P64685 P9WM67 P9WM67 NC_000962.3 Mycobrowser_v5 CDS 109783 110019 . + 0 Rv0100 Rv0100 Function unknown Conserved hypothetical protein Rv0100, (MTCY251.19), len: 78 aa. Conserved hypothetical protein, equivalent only to CAC30950.1|AL583924 conserved hypothetical protein from Mycobacterium leprae (78 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WM65 No conserved hypotheticals P64687 P9WM65 GO:0048037 NC_000962.3 Mycobrowser_v5 CDS 591475 591576 . + 0 Rv0500B Rv0500B Function unknown Conserved hypothetical protein Rv0500B, len: 33 aa. Conserved hypothetical protein. Basic protein 18 of the 33 aa are Arg or Lys, with strong similarity to AL079345|SCE68_25 protein from Streptomyces coelicolor cosmid E6 (32 aa), FASTA scores: opt: 176, E(): 1e-06, (93.1% identity in 29 aa overlap). Same gene arrangement in both actinomycetes. P9WKT5 No conserved hypotheticals P0A5C9 P9WKT5 P9WKT5 NC_000962.3 Mycobrowser_v5 CDS 884072 884800 . - 0 Rv0790c Rv0790c Unknown Hypothetical protein Rv0790c, (MTCY369.34c), len: 242 aa. Hypothetical unknown protein. I6XWA9 No conserved hypotheticals P71843 I6XWA9 I6XWA9 NC_000962.3 Mycobrowser_v5 CDS 133950 136007 . + 0 Rv0111 Rv0111 Function unknown; probably involved in cellular metabolism. Possible transmembrane acyltransferase Rv0111, (MTV031.05), len: 685 aa. Possible transmembrane acyltransferase, equivalent to AA22904.1|AL035300 putative acyltransferase from Mycobacterium leprae (696 aa). Also similar to others e.g. C69975 acyltransferase homolog yrhL from Bacillus subtilis (634 aa), FASTA scores: opt: 520, E(): 4e-22, (36.4% identity in 382 aa overlap). Very similar to Mycobacterium tuberculosis proteins Rv0228, Rv1254, Rv1565c, etc. O53633 No intermediary metabolism and respiration O53633 O53633 GO:0016021,GO:0008415 2.3.1.- O53633 NC_000962.3 Mycobrowser_v5 CDS 119915 122173 . - 0 Rv0103c ctpB Cation-transporting ATPase; possibly catalyzes the transport of a cation (possibly coopper) with the hydrolyse of ATP [catalytic activity: ATP + H(2)O + cation(in) = ADP + orthophosphate + cation(out)]. Probable cation-transporter P-type ATPase B CtpB Rv0103c, (MTCY251.22c), len: 752 aa. Probable ctpB, cation-transporting P-type ATPase B (transmembrane protein), equivalent to CTPB_MYCLE|P46840 cation-transporting P-type ATPase B from Mycobacterium leprae (750 aa), FASTA scores: opt: 3615, E(): 0, (76.5% identity in 752 aa overlap). Also highly similar to others e.g. CAB96031.1|AL360055 putative metal transporter ATPase from Streptomyces coelicolor (753 aa); NP_241423.1|NC_002570 copper-transporting ATPase from Bacillus halodurans (806 aa); etc. Also highly similar to Z46257|MLACEA_7 aceA gene for isocitrate L from Mycobacterium leprae (750 aa), FASTA scores: opt: 3615, E():0, (76.5% identity in 752 aa overlap). And similar to MTCY251.11 from Mycobacterium tuberculosis, FASTA score: (68.3% identity in 742 aa overlap). Contains PS01047 Heavy-metal-associated domain, PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases), subfamily IB. P9WPT9 No cell wall and cell processes Q10877 P9WPT9 GO:0006754,GO:0004008,GO:0016021,GO:0046872,GO:0030001,GO:0005886,GO:0005524 3.6.3.- P9WPT9 NC_000962.3 Mycobrowser_v5 CDS 122317 123831 . + 0 Rv0104 Rv0104 Function unknown Conserved hypothetical protein Rv0104, (MTCY251.23), len: 504 aa. Conserved hypothetical protein, showing weak similarity with other cAMP-dependent protein kinases e.g. AAC37564.1|M65066 cAMP-dependent protein kinase RI-beta regulatory subunit from Homo sapiens (380 aa); etc. P9WM61 No conserved hypotheticals Q10898 P9WM61 GO:0003824,GO:0016021,GO:0008152,GO:0005886 P9WM61 NC_000962.3 Mycobrowser_v5 CDS 123980 124264 . - 0 Rv0105c rpmB1 Possibly involved in a translation mechanism. 50S ribosomal protein L28-1 RpmB1 Rv0105c, (MTCY251.24c), len: 94 aa. rpmB1, 50S ribosomal protein L28-1, highly similar to others e.g. Q9X8K8|R28B_STRCO 50S ribosomal protein L28-2 from Streptomyces coelicolor (78 aa); RL28_ECOLI|P02428 50s ribosomal protein l28 from Escherichia coli (77 aa), FASTA scores: opt: 167, E(): 6.2e-06, (40.7% identity in 59 aa overlap); etc. Also similar to MTCY63A_2 from Mycobacterium tuberculosis. Belongs to the L28P family of ribosomal proteins. P9WHB1 No information pathways P0A5V6 P9WHB1 GO:0003735,GO:0006412,GO:0005840 P9WHB1 NC_000962.3 Mycobrowser_v5 CDS 157847 158302 . + 0 Rv0130 htdZ Unknown Probable 3-hydroxyl-thioester dehydratase Rv0130, (MTCI5.04), len: 151 aa. Probable htdZ, 3-hydroxyl-thioester dehydratase. Forms single hot-dog fold, features R-specific hydratase motif, substrate unknown, forms homodimer. Shows structural similarity to six others in Mycobacterium tuberculosis (see Castell et al (2005) below). Similar to others e.g. AL096811|SCI30A_19 from Streptomyces coelicolor (153 aa), FASTA scores: opt: 639, E(): 0, (60.8% identity in 148 aa overlap). P9WNP3 No intermediary metabolism and respiration 2C2I P96807 P9WNP3 GO:0006631,GO:0016491,GO:0004300 4.2.1.17 P9WNP3 NC_000962.3 Mycobrowser_v5 CDS 778990 779487 . + 0 Rv0678 Rv0678 Function unknown Conserved protein Rv0678, (MTV040.06), len: 165 aa. Conserved protein, showing weak similarity with AL049754|SCH10_10 hypothetical protein from Streptomyces coelicolor (152 aa), FASTA scores: opt: 149, E(): 0.0018, (22.9% identity in 140 aa overlap). I6Y8F7 No conserved hypotheticals I6Y8F7 I6Y8F7 NC_000962.3 Mycobrowser_v5 CDS 2258273 2258671 . + 0 Rv2010 vapC15 Unknown Toxin VapC15 Rv2010, (MTCY39.07c), len: 132 aa. VapC15, toxin, part of toxin-antitoxin (TA) operon with Rv2009, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). P9WF97 No virulence, detoxification, adaptation P64925 P9WF97 P9WF97 NC_000962.3 Mycobrowser_v5 CDS 125643 130541 . - 0 Rv0107c ctpI Cation-transporting ATPase; possibly catalyzes the transport of a cation (possibly magnesium) with the hydrolyse of ATP [catalytic activity: ATP + H(2)O + cation(in) = ADP + phosphate + cation(out)]. Probable cation-transporter ATPase I CtpI Rv0107c, (MTCY251.26c, MTV031.01c), len: 1632 aa. Probable ctpI, cation-transporting ATPase I P-type, highly similar to NP_302704.1|NC_002677 probable cation transport ATPase from Mycobacterium leprae (1609 aa); and similar to others e.g. CAB69720.1|AL137166 putative transport ATPase from Streptomyces coelicolor (1472 aa); ATA1_SYNY|P37367 cation-transporting ATPase pma1 from Synechocystis sp. (915 aa), FASTA scores: opt: 603, E(): 6.6e-29, (32.4% identity in 710 aa overlap); etc. Also similar to MTCY39.21c and MTCY22G10.22c from Mycobacterium tuberculosis, FASTA score: (34.4% identity in 796 aa overlap). Contains PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases). P9WPS5 No cell wall and cell processes Q10900 P9WPS5 GO:0006754,GO:0016021,GO:0015693,GO:0015444,GO:0046872,GO:0005886,GO:0005524 3.6.3.- P9WPS5 NC_000962.3 Mycobrowser_v5 CDS 130895 131104 . - 0 Rv0108c Rv0108c Unknown Hypothetical protein Rv0108c, (MTV031.02c), len: 69 aa. Hypothetical unknown protein. O53630 No conserved hypotheticals O53630 NC_000962.3 Mycobrowser_v5 CDS 133020 133769 . + 0 Rv0110 Rv0110 Unknown Probable conserved integral membrane protein Rv0110, (MTV031.04), len: 249 aa. Probable conserved integral membrane protein, similar to many e.g. AL079308|SCH69_25 from Streptomyces coelicolor (297 aa), FASTA scores: opt: 552, E(): 6.1e-29, (45.4% identity in 251 aa overlap); P54493|YQGP_BACSU hypothetical 56.4 KD protein from Bacillus subtilis (507 aa), FASTA scores: opt: 320, E(): 4e-15, (32.4% identity in 210 aa overlap); etc. O53632 No cell wall and cell processes O53632 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 136289 137245 . + 0 Rv0112 gca Possibly involved in synthesis of a-band common antigen lipopolysaccharide. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose [catalytic activity: GDP-mannose = GDP-4-dehydro-6-deoxy-D-mannose + H2O]. Possible GDP-mannose 4,6-dehydratase Gca (GDP-D-mannose dehydratase) Rv0112, (MTV031.06), len: 318 aa. Possible gca, GDP-mannose 4,6-dehydratase, similar to others e g. U18320|PAU18320_1 GDP-D-mann from Pseudomonas aeruginosa (323 aa), FASTA scores: opt: 415, E(): 4.4e-21, (27.0% identity in 318 aa overlap). Similar to Rv3634c, Rv3784, etc from Mycobacterium tuberculosis. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Seems to belong to the GDP-mannose 4,6-dehydratase family. Cofactor: NAD(+). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53634 No cell wall and cell processes O53634 O53634 GO:0044237,GO:0050662,GO:0016491,GO:0008446 4.2.1.47 O53634 NC_000962.3 Mycobrowser_v5 CDS 677710 677925 . + 0 Rv0581 vapB26 Unknown Possible antitoxin VapB26 Rv0581, (MTV039.19), len: 71 aa. Possible vapB26, antitoxin, part of toxin-antitoxin (TA) operon with Rv0582, see Arcus et al. 2005. Showing weak similarity to other Mycobacterium tuberculosis proteins including P95003|Z83863|Rv2550c|MTCY159_6 conserved hypothetical protein (81 aa), FASTA scores: opt: 93, E(): 3.2, (25.7% identity in 70 aa overlap); Rv2871; Rv1241; etc. Also shows weak similarity to X05648|SGSPH_1 from Streptomyces glaucescens (77 aa), FASTA scores: opt: 92, E(): 3.6, (35.4% identity in 65 aa overlap). O53778 No virulence, detoxification, adaptation O53778 O53778 GO:0006355,GO:0003677 O53778 NC_000962.3 Mycobrowser_v5 CDS 138513 139673 . + 0 Rv0115 hddA Involved in biosynthesis of nucleotide-activated glycero-manno-heptose (D-alpha-D pathway) [catalytic activity: D-glycero-alpha,beta-D-manno-heptose 7-phosphate + ATP = D-glycero-alpha-D-manno-heptose 1,7-biphosphate]. Possible D-alpha-D-heptose-7-phosphate kinase HddA Rv0115, (MTV031.09), len: 386 aa. Possible hddA, D-alpha-D-heptose-7-phosphate kinase (see citation below), similar to several hypothetical proteins and sugar kinases e.g. AAK27850.1|AF324836_3 D-glycero-D-manno-heptose 7-phosphate kinase from Aneurinibacillus thermoaerophilus (341 aa); AAK80995.1|AE007802_11 Sugar kinase from Clostridium acetobutylicum (364 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53637 No cell wall and cell processes O53637 O53637 GO:0016301,GO:0016310,GO:0005524 2.-.-.- O53637 NC_000962.3 Mycobrowser_v5 CDS 140267 141022 . - 0 Rv0116c ldtA Involved in peptidoglycan synthesis. Catalyzes the formation of 3->3 crosslinks between peptidoglycan subunits. Probable L,D-transpeptidase LdtA Rv0116c, (MTV031.10c), len: 251 aa. Probable ldtA, L,D-transpeptidase, showing similarity to several hypothetical mycobacterial proteins e.g. Rv1433 from Mycobacterium tuberculosis (271 aa); and Q49706|B1496_F2_81|U00013 from Mycobacterium leprae (271 aa); to the C-terminal regions of others like Rv0192 from Mycobacterium tuberculosis (366 aa), FASTA scores: opt: 451, E(): 1.7e-21, (46.7% identity in 270 aa overlap); and Rv0192|Z97050|MTCI28_32 from Mycobacterium tuberculosis cosmid (366 aa), FASTA scores: opt: 699, E(): 0, (45.7% identity in 221 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). O53638 No cell wall and cell processes O53638 O53638 O53638 NC_000962.3 Mycobrowser_v5 CDS 141200 142144 . + 0 Rv0117 oxyS Could effect functions of OXYR during evolution. Oxidative stress response regulatory protein OxyS Rv0117, (MTV031.11), len: 314 aa. OxyS, oxidative stress response protein regulatory protein, LysR family (see citation below). Similar to many transcription regulators and OxyR, the oxidative stress response protein of many bacteria. Contains LysR family signature at N-terminus. Also contains helix-turn-helix motif at aa 16-37 (Score 1543, +4.44 SD). Belongs to the LysR family of transcriptional regulators. OXYR is required for the induction of a regulon of hydrogen peroxide inducible genes such as catalase, glutathione-reductase, etc. L7N677 No regulatory proteins O33175 L7N677 GO:0006350,GO:0003700,GO:0006355 L7N677 NC_000962.3 Mycobrowser_v5 CDS 158315 159658 . - 0 Rv0131c fadE1 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE1 Rv0131c, (MTCI5.05c), len: 447 aa. Probable fadE1, acyl-CoA dehydrogenase, similar to many e.g. ACDS_HUMAN|P16219 acyl-CoA dehydrogenase short-chain specific precursor (412 aa), FASTA scores: opt: 522, E(): 1.4e-23, (30.1% identity in 425 aa overlap). Also highly similar to MTCI5_28 from Mycobacterium tuberculosis. P96808 No lipid metabolism P96808 P96808 GO:0003995,GO:0055114,GO:0050660 1.3.99.- P96808 NC_000962.3 Mycobrowser_v5 CDS 171215 172168 . + 0 Rv0145 Rv0145 Possible methyltransferase Possible S-adenosylmethionine-dependent methyltransferase Rv0145, (MTCI5.19), len: 317 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), highly similar to many e.g. CAC32172.1|AL583926 conserved hypothetical protein from Mycobacterium leprae (310 aa); and several Mycobacterium tuberculosis proteins e.g. Rv0726c, Rv0731c, etc. P9WFJ1 No lipid metabolism P96822 P9WFJ1 GO:0008168 2.1.1.- P9WFJ1 NC_000962.3 Mycobrowser_v5 CDS 144049 145626 . + 0 Rv0119 fadD7 Function unknown, but involved in lipid degradation. Probable fatty-acid-CoA ligase FadD7 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv0119, (MTV031.13-MTCI418B.01), len: 525 aa. Probable fadD7, fatty-acid-CoA synthetase, similar to 4-coumarate:CoA ligase of many organisms e.g. U39405|PTU39405_1 4-coumarate:CoA ligase from Pinus taedaxylem (537 aa), FASTA scores: opt: 483, E(): 8.3e-22, (28.2% identity in 440 aa overlap). Contains PS00455 Putative AMP-binding domain signature. O07169 No lipid metabolism O07169 O07169 GO:0008152,GO:0016874 6.2.1.- O07169 NC_000962.3 Mycobrowser_v5 CDS 145627 147771 . - 0 Rv0120c fusA2 Involved in translation mechanism. This protein may promote the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. Probable elongation factor G FusA2 (EF-G) Rv0120c, (MTCI418B.02c), len: 714 aa. Probable fusA2 (alternate gene name: fus2), elongation factor G, highly similar to others e.g. EFG_ECOLI|P02996 elongation factor G (ef-g) from Escherichia coli (703 aa), FASTA scores: opt: 1049, E(): 0, (32.5% identity in 717 aa overlap). Also similar to fusA1|MTCY210.01 from Mycobacterium tuberculosis FASTA score: (39.1% identity in 299 aa overlap); and P30767|EFG_MYCLE elongation factor G (EF-G) from Mycobacterium leprae (701 aa), FASTA score: (31.7% identity in 710 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the GTP-binding elongation factor family, EF-G/EF-2 subfamily. P9WNM9 No information pathways O07170 P9WNM9 GO:0003924,GO:0005525 P9WNM9 NC_000962.3 Mycobrowser_v5 CDS 147908 148342 . - 0 Rv0121c Rv0121c Function unknown Conserved protein Rv0121c, (MTCI418B.03c), len: 144 aa. Conserved protein, showing some similarity with others proteins from Mycobacterium tuberculosis e.g. Rv1155, Rv1875, Rv2074, etc. O07171 No conserved hypotheticals O07171 O07171 GO:0010181 O07171 NC_000962.3 Mycobrowser_v5 CDS 148491 148859 . + 0 Rv0122 Rv0122 Unknown Hypothetical protein Rv0122, (MTCI418B.04), len: 122 aa. Hypothetical unknown protein. O07172 No conserved hypotheticals O07172 O07172 NC_000962.3 Mycobrowser_v5 CDS 148856 149224 . + 0 Rv0123 Rv0123 Unknown Unknown protein Rv0123, (MTCI418B.05), len: 122 aa. Unknown protein. O07173 No conserved hypotheticals O07173 O07173 GO:0006355,GO:0003677 NC_000962.3 Mycobrowser_v5 CDS 149533 150996 . + 0 Rv0124 PE_PGRS2 Function unknown PE-PGRS family protein PE_PGRS2 Rv0124, (MTCI418B.06), len: 487 aa. PE_PGRS2, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), highly similar to many e.g. Y0DP_MYCTU|Q50615 from Mycobacterium tuberculosis (498 aa), FASTA scores: opt: 1730, E(): 0, (60.7% identity in 504 aa overlap). Q79G08 No PE/PPE Q79G08 Q79G08 Q79G08 NC_000962.3 Mycobrowser_v5 CDS 289345 289782 . + 0 Rv0240 vapC24 Unknown Possible toxin VapC24. Contains PIN domain. Rv0240, (MTV034.06), len: 145 aa. Possible vapC24, toxin, part of toxin-antitoxin (TA) operon with Rv0239, contains PIN domain, weak similarity with Rv3697c from Mycobacterium tuberculosis (145 aa). See Arcus et al. 2005. FASTA scores: opt: 145, E(): 7.6e-05, (28.0% identity in 143 aa overlap). P9WF87 No virulence, detoxification, adaptation O53663 P9WF87 P9WF87 NC_000962.3 Mycobrowser_v5 CDS 677922 678329 . + 0 Rv0582 vapC26 Unknown Possible toxin VapC26. Contains PIN domain. Rv0582, (MTV039.20), len: 135 aa. Possible vapC26, toxin, part of toxin-antitoxin (TA) operon with Rv0581, contains PIN domain, see Arcus et al. 2005. O53779 No virulence, detoxification, adaptation O53779 O53779 O53779 NC_000962.3 Mycobrowser_v5 CDS 154232 155599 . + 0 Rv0127 mak Catalyzes the transfer of a phosphate group from ATP to maltose to produce maltose-1-phosphate [catalytic activity: ATP + maltose = ADP + maltose-1-phosphate] Maltokinase Mak Rv0127, (MTCI418B.09, MTCI5.01), len: 455 aa. Mak, maltokinase; highly similar to various proteins e.g. AJ0012|SCJ001205_4 hypothetical protein from Streptomyces coelicolor A3(2) (464 aa), FASTA scores: opt: 412, E(): 1.1e-19, (40.6% identity in 485 aa overlap); AJ0012|SCJ001206_5 hypothetical protein from Streptomyces coelicolor A3(2) (453 aa), FASTA scores: opt: 403, E(): 4.3 e-19, (36.5% identity in 455 aa overlap). O07177 No virulence, detoxification, adaptation O07177 O07177 O07177 NC_000962.3 Mycobrowser_v5 CDS 176665 176952 . - 0 Rv0150c Rv0150c Function unknown Conserved hypothetical protein Rv0150c, (MTCI5.24c), len: 95 aa. Conserved hypothetical protein, showing some similarity with C-terminus of O53949|Rv1800|MTV049.22 PPE-family protein from Mycobacterium tuberculosis (655 aa), FASTA score: (36.5% identity in 104 aa overlap). P96827 No conserved hypotheticals P96827 P96827 NC_000962.3 Mycobrowser_v5 CDS 155667 156446 . + 0 Rv0128 Rv0128 Unknown Probable conserved transmembrane protein Rv0128, (MTCI5.02), len: 259 aa. Probable conserved transmembrane protein, with some similarity to Rv3064c and other bacterial proteins e.g. AAK85977.1|AE007957|AGR_C_254p from Agrobacterium tumefaciens (206 aa). P96805 No cell wall and cell processes P96805 P96805 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 208417 208971 . + 0 Rv0177 Rv0177 Unknown Probable conserved Mce associated protein Rv0177, (MTCI28.17), len: 184 aa. Probable conserved Mce-associated protein, equivalent to CAC32129.1|AL583926 conserved membrane protein from Mycobacterium leprae (184 aa). Also similar to mce-associated proteins from Mycobacterium tuberculosis e.g. Rv1363c, Rv1973, Rv3493c, etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O07421 No conserved hypotheticals O07421 NC_000962.3 Mycobrowser_v5 CDS 257783 258856 . - 0 Rv0215c fadE3 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE3 Rv0215c, (MTCY08D5.10c), len: 357 aa. Probable fadE3, acyl- dehydrogenase, similar to many e.g. ACDB_BACSU|P45857 acyl-CoA dehydrogenase from B. subtilis (379 aa), FASTA scores: opt: 812, E(): 0, (39.5% identity in 354 aa overlap). P96397 No lipid metabolism P96397 P96397 GO:0003995,GO:0055114,GO:0050660 1.3.99.- P96397 NC_000962.3 Mycobrowser_v5 CDS 475816 476184 . + 0 Rv0397 Rv0397 Function unknown Conserved 13E12 repeat family protein Rv0397, (MTCY04D9.09), len: 122 aa. Part of 13E12 repeat family of conserved Mycobacterium tuberculosis proteins, similar to downstream Rv0393|Z84725|MTCY4D9_5 conserved 13E12 repeat family protein (441 aa), FASTA scores: E(): 0, (87.7% identity in 122 aa overlap). P95205 No insertion seqs and phages P95205 P95205 NC_000962.3 Mycobrowser_v5 CDS 2263426 2264001 . + 0 Rv2016 Rv2016 Unknown Hypothetical protein Rv2016, (MTV018.03), len: 191 aa. Hypothetical protein. O53462 No conserved hypotheticals O53462 NC_000962.3 Mycobrowser_v5 CDS 159700 160782 . - 0 Rv0132c fgd2 Catalyzes oxidation of glucose-6-phosphate to 6-phosphogluconolactone using coenzyme F420 (an *-hydroxy-5-deazaflavin derivative) as the electron acceptor. Putative F420-dependent glucose-6-phosphate dehydrogenase Fgd2 Rv0132c, (MTCI5.06c), len: 360 aa. Putative fgd2, F420-dependent glucose-6-phosphate dehydrogenase, highly similar to many from Mycobacteria e.g. AAD38167|g5031431 from Mycobacterium chelonae. Also similar to MJ1534|Q58929 N5,N10-methylene tetrahydromethanopterin reductase from methanococcus jannaschii (342 aa), FASTA scores: opt: 285, E(): 7.9e-11, (28.4% identity in 292 aa overlap). And also similar to Rv0953c, Rv0791c, etc from Mycobacterium tuberculosis. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. P96809 No intermediary metabolism and respiration P96809 P96809 GO:0016491,GO:0055114 1.-.-.- P96809 NC_000962.3 Mycobrowser_v5 CDS 160869 161474 . + 0 Rv0133 Rv0133 Acetylation, substrate unknown GCN5-related N-acetyltransferase Rv0133, (MTCI5.07), len: 201 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain in C-terminal part. See Vetting et al. 2005. Highly similar to others e.g. PUAC_STRLP|P13249 puromycyn N-acetyltransferase (199 aa), FASTA scores: opt: 341, E(): 1.8e-16, (33.3% identity in 201 aa overlap). P96810 No intermediary metabolism and respiration P96810 P96810 GO:0008152,GO:0008080 2.3.1.- P96810 NC_000962.3 Mycobrowser_v5 CDS 161771 162673 . + 0 Rv0134 ephF Thought to be involved in detoxification reactions following oxidative damage to lipids [catalytic activity: an epoxide + H(2)O = a glycol]. Possible epoxide hydrolase EphF (epoxide hydratase) (arene-oxide hydratase) Rv0134, (MTCI5.08), len: 300 aa. Possible ephE, epoxide hydrolase (see citation below), similar to others e.g. Q39856 epoxide hydrolase (341 aa), FASTA scores: opt: 369, E(): 4.6e-17, (27.2% identity in 335 aa overlap); etc. Also similar to MTCY09F9.26c from Mycobacterium tuberculosis (29.5% identity in 346 aa overlap). P96811 No virulence, detoxification, adaptation P96811 P96811 GO:0016787 3.3.2.3 P96811 NC_000962.3 Mycobrowser_v5 CDS 196861 197658 . + 0 Rv0167 yrbE1A Unknown Conserved integral membrane protein YrbE1A Rv0167, (MTCI28.07), len: 265 aa. YrbE1A, unknown integral membrane protein, part of mce1 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07791|Rv0587|MTCY19H5.35|yrbE2A (265 aa); O53965|Rv1964|MTV051.02|yrbE3A (265 aa); etc. Also highly similar or similar to conserved hypothetical integral membrane proteins of yrbEA type, e.g. NP_302654.1|NC_002677 conserved membrane protein from Mycobacterium leprae (267 aa); P45030|YRBE_HAEIN|HI1086 hypothetical protein from Haemophilus influenzae (261 aa), FASTA scores: opt: 328, E(): 1.8e-15, (26.6% identity in 244 aa overlap); etc. O07412 No virulence, detoxification, adaptation O07412 O07412 O07412 NC_000962.3 Mycobrowser_v5 CDS 2545332 2545631 . + 0 Rv2271 Rv2271 Unknown Conserved hypothetical protein Rv2271, (MTCY339.39c), len: 99 aa. Conserved hypothetical protein; some similarity to hypothetical protein AAK01340.1|AF265275_3 (AF265275) from uncultured organism Pu8 (104 aa) E= 4e-10, (34% identity in 91 aa overlap) P9WLF7 No conserved hypotheticals P9WLF7 NC_000962.3 Mycobrowser_v5 CDS 237892 238395 . - 0 Rv0201c Rv0201c Function unknown Conserved protein Rv0201c, (MTV033.09c), len: 167 aa. Conserved protein, equivalent to Z95398|MLCL622.15c from Mycobacterium leprae (170 aa), FASTA scores: opt: 646, E(): 0, (63.9% identity in 158 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53652 No conserved hypotheticals O53652 O53652 NC_000962.3 Mycobrowser_v5 CDS 2833510 2833761 . - 0 Rv2517c Rv2517c Unknown Unknown protein Rv2517c, (MTV009.02c), len: 83 aa. Unknown protein. Equivalent to AAK46899 from Mycobacterium tuberculosis strain CDC1551 (97 aa) but shorter 14 aa. O53222 No conserved hypotheticals O53222 NC_000962.3 Mycobrowser_v5 ncRNA 293604 293705 . + 0 MTB000051 F6 Unknown Putative small regulatory RNA F6, putative small regulatory RNA (See Arnvig and Young, 2009; DiChiara et al., 2010). Alternate 3'-ends at positions 293641 and 293661. No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 165827 166849 . + 0 Rv0139 Rv0139 Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0139, (MTCI5.13), len: 340 aa. Possible oxidoreductase, similar to others e.g. O34285|HPNA HPNA protein from Zymomonas mobilis (337 aa), FASTA scores: opt: 507, E (): 5.8e-27, (31.1% identity in 328 aa overlap); TRE_STRGR|P29782 dtdp-glucose 4,6-dehydratase (328 aa), FASTA scores: opt: 254, E(): 2.6e-10, (29.0% identity in 307 aa overlap). P96816 No intermediary metabolism and respiration P96816 P96816 GO:0050662,GO:0055114,GO:0016491,GO:0044237 1.-.-.- P96816 NC_000962.3 Mycobrowser_v5 CDS 170284 171126 . + 0 Rv0144 Rv0144 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (possibly TetR-family) Rv0144, (MTCI5.18), len: 280 aa. Probable transcriptional regulator, possibly TetR family. Has region similar to others e.g. Q59431|UIDR_ECOLI|GUSR|B1618|Z2623|ECS2326 UID operon repressor (GUS operon) from Escherichia coli strains K12 and O157:H7 (196 aa), FASTA scores: opt: 214, E(): 1.1e-06, (26.0% identity in 196 aa overlap). Contains probable helix-turn helix motif from aa 109-130 (Score 1463, +4.17 SD). Could belong to the TetR/AcrR family of transcriptional regulators. P96821 No regulatory proteins P96821 P96821 GO:0006350,GO:0003700,GO:0006355 P96821 NC_000962.3 Mycobrowser_v5 CDS 172211 173143 . + 0 Rv0146 Rv0146 Possible methyltransferase Possible S-adenosylmethionine-dependent methyltransferase Rv0146, (MTCI5.20), len: 310 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), highly similar to others e.g. AC30975.1|AL583924 conserved hypothetical protein from Mycobacterium leprae (304 aa); and several Mycobacterium tuberculosis proteins e.g. Rv0726c, Rv0731c, etc. P9WFJ3 No lipid metabolism P96823 P9WFJ3 GO:0008168 2.1.1.- P9WFJ3 NC_000962.3 Mycobrowser_v5 CDS 223607 223909 . + 0 Rv0192A Rv0192A Function unknown Conserved secreted protein Rv0192A, len: 100 aa. Probable N-terminal part of Rv0192, which is member of family P5.17 with Rv0116c, Rv1433, Rv2518c, Rv0483. These are all predicted to be exported/membrane proteins. Rv0192A has typical N-terminal signal peptide which is functional and was identified by PhoA fusion screens: O52054 PGB14T-O1 precursor (fragment 45 AA) (see Chubb et al., 1998). Since Rv0192 misses a signal peptide this suggests that there is a frameshift in the region of the overlap with Rv0192 but none found on reinspection of sequence. Q79FZ8 No cell wall and cell processes Q79FZ8 NC_000962.3 Mycobrowser_v5 CDS 1027104 1027685 . + 0 Rv0921 Rv0921 Prevents the cointegration of foreign DNA before integration into the chromosome. Possible resolvase Rv0921, (MTCY21C12.15), len: 193 aa. Possible resolvase for IS1535, highly similar to many bacterial resolvases e.g. MTCY274.17c|YX1C_MYCTU Q10831 from Mycobacterium tuberculosis (295 aa), FASTA scores: opt: 537, E(): 5.7e-29, (51.8% identity in 166 aa overlap). Presents an helix turn helix motif. I6WZS4 No insertion seqs and phages O05913 I6WZS4 GO:0006310,GO:0000150,GO:0003677 I6WZS4 NC_000962.3 Mycobrowser_v5 promoter 174782 174803 . + 0 Rv0148 Rv0148 Function unknown; possibly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv0148, (MTCI5.22), len: 286 aa. Probable short-chain dehydrogenase, similar to others, in particular Estradiol 17 beta-dehydrogenases, e.g. DHB4_MOUSE|P51660 estradiol 17 beta-dehydrogenase 4 (735 aa), FASTA scores: opt: 952, E(): 0, (52.5% identity in 276 aa overlap). Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. P96825 No intermediary metabolism and respiration P96825 P96825 GO:0055114,GO:0016491 1.-.-.- P96825 NC_000962.3 Mycobrowser_v5 CDS 174833 175693 . + 0 Rv0148 Rv0148 Function unknown; possibly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv0148, (MTCI5.22), len: 286 aa. Probable short-chain dehydrogenase, similar to others, in particular Estradiol 17 beta-dehydrogenases, e.g. DHB4_MOUSE|P51660 estradiol 17 beta-dehydrogenase 4 (735 aa), FASTA scores: opt: 952, E(): 0, (52.5% identity in 276 aa overlap). Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. P96825 No intermediary metabolism and respiration P96825 P96825 GO:0055114,GO:0016491 1.-.-.- P96825 NC_000962.3 Mycobrowser_v5 CDS 175700 176668 . + 0 Rv0149 Rv0149 Possibly binds NADP and acts through a one-electron transfer process. Quinones are supposed to be the best substrates. May act in the detoxification of xenobiotics [catalytic activity: NADPH + quinone = NADP+ + semiquinone] Possible quinone oxidoreductase (NADPH:quinone oxidoreductase) (zeta-crystallin) Rv0149, (MTCI5.23), len: 322 aa. Possible quinone oxidoreductase, similar to others oxidoreductases e.g. Q08257 quinone oxidoreductase (329 aa), FASTA scores: opt: 397, E(): 3.2e-18, (28.4% identity in 328 aa overlap); SCHCOADH_4 from Streptomyces coelicolor. Also similar to many proteins from Mycobacterium tuberculosis. Contains PS01162 Quinone oxidoreductase / zeta-crystallin signature. Belongs to the zinc-containing alcohol dehydrogenase family, quinone oxidoreductase subfamily. P96826 No intermediary metabolism and respiration P96826 P96826 GO:0055114,GO:0008270,GO:0003960 1.6.5.5 P96826 NC_000962.3 Mycobrowser_v5 CDS 209703 210812 . - 0 Rv0179c lprO Unknown Possible lipoprotein LprO Rv0179c, (MTCI28.19c), len: 369 aa. Possible lprO, lipoprotein (visibly not conserved). Contains possible N-terminal signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O07423 No cell wall and cell processes O07423 NC_000962.3 Mycobrowser_v5 CDS 214969 215718 . + 0 Rv0184 Rv0184 Function unknown Conserved hypothetical protein Rv0184, (MTCI28.24), len: 249 aa. Conserved hypothetical protein, equivalent to CAC32136.1|AL583926 conserved hypothetical protein from Mycobacterium leprae (249 aa); and C-terminus highly similar to CAB08793.1|Z95398 conserved hypothetical protein from Mycobacterium leprae (145 aa), FASTA scores: E(): 0, (75.2 identity in 145 aa overlap). Also similar to 049841|SCE9_39|T36358 hypothetical protein from Streptomyces coelicolor (418 aa), FASTA scores: opt: 231, E(): 8.1e-08, (30.4% identity in 270 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O07428 No conserved hypotheticals O07428 NC_000962.3 Mycobrowser_v5 CDS 424269 424694 . - 0 Rv0354c PPE7 Function unknown PPE family protein PPE7 Rv0354c, (MTCY13E10.14c), len: 141 aa. PPE7, Member of the Mycobacterium tuberculosis PPE family, similar to others e.g. MTCY63_9 from Mycobacterium tuberculosis (2411 aa), FASTA scores: E(): 3.6e-11, (47.6% identity in 103 aa overlap). Possible continuation of ORF upstream, but no sequence error apparent. L0T545 No PE/PPE L0T545 NC_000962.3 Mycobrowser_v5 CDS 181987 183198 . - 0 Rv0154c fadE2 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE2 Rv0154c, (MTCI5.28c), len: 403 aa. Probable fadE2, acyl-CoA dehydrogenase, similar to many e.g. C-terminal region of O01590 acyl-CoA dehydrogenase (974 aa), FASTA scores: opt: 1150, E(): 0, (50.0% identity in 402 aa overlap); ACDS_MEGEL|Q06319 acyl-CoA dehydrogenase (short-chain) (383 aa), FASTA score: (35.0% identity in 306 aa overlap). Could belong to the acyl-CoA dehydrogenases family. P96831 No lipid metabolism P96831 P96831 GO:0003995,GO:0055114,GO:0050660 1.3.99.- P96831 NC_000962.3 Mycobrowser_v5 CDS 183622 184722 . + 0 Rv0155 pntAa The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [catalytic activity: NADPH + NAD+ = NADP+ + NADH]. Probable NAD(P) transhydrogenase (subunit alpha) PntAa [first part; catalytic part] (pyridine nucleotide transhydrogenase subunit alpha) (nicotinamide nucleotide transhydrogenase subunit alpha) Rv0155, (MTCI5.29), len: 366 aa. Probable pntAa, first part of NAD(P) transhydrogenase subunit alpha, similar to N-terminus of others e.g. PNTA_ECOLI|P07001|P76888|B1603 NAD (P) transhydrogenase subunit alpha from Escherichia coli strain K12 (510 aa), FASTA scores: opt: 921, E(): 0, (42.1% identity in 361 aa overlap); proton-translocating nicotinamide nucleotide transhydrogenase subunit PNTAA. P96832 No intermediary metabolism and respiration P96832 P96832 GO:0055114,GO:0008750 1.6.1.2 P96832 NC_000962.3 Mycobrowser_v5 CDS 210892 212250 . - 0 Rv0180c Rv0180c Unknown Probable conserved transmembrane protein Rv0180c, (MTCI28.20c), len: 452 aa. Probable conserved transmembrane protein, equivalent to CAC32132.1|AL583926 probable conserved membrane protein from Mycobacterium leprae (465 aa). Shows some similarity with others membrane proteins e.g. AL096849|SCI11_29 from Streptomyces coelicolor (354 aa), FASTA scores: opt: 190, E(): 0.00067, (25.9% identity in 409 aa overlap). O07424 No cell wall and cell processes O07424 GO:0016021 O07424 NC_000962.3 Mycobrowser_v5 CDS 224724 226571 . - 0 Rv0193c Rv0193c Unknown Hypothetical protein Rv0193c, (MTV033.01c-MTCI28.32), len: 615 aa. Hypothetical unknown protein. O07437 No conserved hypotheticals O07437 O07437 NC_000962.3 Mycobrowser_v5 CDS 249038 250123 . + 0 Rv0209 Rv0209 Unknown Hypothetical protein Rv0209, (MTCY08D5.04), len: 361 aa. Hypothetical unknown protein. P96391 No conserved hypotheticals P96391 P96391 NC_000962.3 Mycobrowser_v5 CDS 1593505 1593978 . + 0 Rv1419 Rv1419 Unknown Unknown protein Rv1419, (MTCY21B4.37), len: 157 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). P9WLX9 No conserved hypotheticals P64849 P9WLX9 GO:0005529,GO:0016021 P9WLX9 NC_000962.3 Mycobrowser_v5 CDS 184723 185055 . + 0 Rv0156 pntAb The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [catalytic activity: NADPH + NAD+ = NADP+ + NADH]. Probable NAD(P) transhydrogenase (subunit alpha) PntAb [second part; integral membrane protein] (pyridine nucleotide transhydrogenase subunit alpha) (nicotinamide nucleotide transhydrogenase subunit alpha) Rv0156, (MTCI5.30), len: 110 aa. Probable pntAb, second part of NAD(P) transhydrogenase subunit alpha, integral membrane protein, similar to C-terminus of others e.g. Q59764 nicotinamide nucleotide transhydrogenase subunit PNTAB (139 aa), FASTA scores: opt: 247, E(): 1.9e-11, (45.5% identity in 88 aa overlap). P96833 No intermediary metabolism and respiration P96833 P96833 NC_000962.3 Mycobrowser_v5 CDS 186785 187429 . + 0 Rv0158 Rv0158 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (possibly TetR-family) Rv0158, (MTV032.01), len: 214 aa. Probable transcriptional regulator, possibly TetR family, showing weak similarity to various transcriptional activators and repressors e.g. P32398|YIXD_BACSU|YHGD hypothetical transcriptional regulatory protein from Bacillus subtilis (191 aa), FASTA scores: opt:172, E(): 2.4e-05, (23.0% identity in 191 aa overlap). Contains helix-turn-helix motif at aa 32-53 (Score 1296, +3.60 SD). Could belong to the TetR/AcrR family of transcriptional regulators. O53641 No regulatory proteins O53641 O53641 GO:0006355,GO:0006350,GO:0003700,GO:0016481 O53641 NC_000962.3 Mycobrowser_v5 CDS 187433 188839 . - 0 Rv0159c PE3 Function unknown PE family protein PE3 Rv0159c, (MTV032.02c), len: 468 aa. PE3, Member of the Mycobacterium tuberculosis PE family (see citation below), similar to many other PE proteins e.g. O06828 from Mycobacterium tuberculosis (528 aa), FASTA scores: opt: 1163, E(): 0, (45.8% identity in 467 aa overlap). Also highly similar to upstream MTV032_3, and to MTCI5_25, MTCI5_26, MTV049_ 21, MTCY1A10_26, etc. Q79G04 No PE/PPE Q79G04 Q79G04 Q79G04 NC_000962.3 Mycobrowser_v5 CDS 188931 190439 . - 0 Rv0160c PE4 Function unknown PE family protein PE4 Rv0160c, (MTV032.03c), len: 502 aa. PE4, Member of the Mycobacterium tuberculosis PE family (see citation below), similar to many other PE proteins e.g. Z92770|MTCI5_26c from Mycobacterium tuberculosis (525 aa), FASTA scores: opt: 816, E(): 0, (41.4% identity in 367 aa overlap); C-terminal region of O06801|RV1768|MTCY28.34 from Mycobacterium tuberculosis (618 aa), FASTA scores: opt: 417, E(): 6.7e-18, (53.5% identity in 142 aa overlap). Also highly similar to downstream ORF MTV032_2. L7N661 No PE/PPE Q7DAC9 L7N661 L7N661 NC_000962.3 Mycobrowser_v5 CDS 212277 213011 . - 0 Rv0181c Rv0181c Function unknown Conserved hypothetical protein Rv0181c, (MTCI28.21c), len: 244 aa. Conserved hypothetical protein, highly similar to other hypothetical proteins e.g. YHHW_ECOLI|P46852 hypothetical 26.3 kd protein from Escherichia coli (231 aa), FASTA scores: opt: 479, E(): 1.2e-29, (37.3% identity in 233 aa overlap); P73623|SLL1773 hypothetical 25.7 kDa protein from Synechocystis sp. strain PCC 6803 (232 aa), FASTA score: (39.1% identity in 233 aa overlap). P9WI85 No conserved hypotheticals P65724 P9WI85 P9WI85 NC_000962.3 Mycobrowser_v5 CDS 262254 262802 . + 0 Rv0219 Rv0219 Unknown Probable conserved transmembrane protein Rv0219, (MTCY08D5.14), len: 182 aa. Probable conserved transmembrane protein, showing similarity with CAB76992.1|AL159178 putative lipoprotein from Streptomyces coelicolor (163 aa). P96401 No cell wall and cell processes P96401 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 552026 552550 . + 0 Rv0461 Rv0461 Unknown Probable transmembrane protein Rv0461, (MTV038.05), len: 174 aa (start uncertain). Probable transmembrane protein. Nucleotide position 552085 in the genome sequence has been corrected, A:G resulting in Q20Q. I6X961 No cell wall and cell processes I6X961 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 191984 193135 . - 0 Rv0162c adhE1 Dehydrogeneses a alcohol (OXIDO-reduction) [catalytic activity: an alcohol + NAD+ = an aldehyde or ketone + NADH]. Probable zinc-type alcohol dehydrogenase (E subunit) AdhE1 Rv0162c, (MTCI28.02c), len: 383 aa. Probable adhE1, zinc-type alcohol dehydrogenase, similar to others e.g. ADH_MACMU|P28469 alcohol dehydrogenase alpha chain (374 aa), FASTA scores: opt: 619, E(): 0, (34.7% identity in 363 aa overlap). Also similar to other alcohol dehydrogenases from Mycobacterium tuberculosis e.g. MTCY369.06c FASTA score: (34.0% identity in 365 aa overlap), MTV022_9 FASTA score: (35.0% identity in 371 aa overlap). Contains PS00059 Zinc-containing alcohol dehydrogenases signature. Belongs to the zinc-containing alcohol dehydrogenase family, class-I subfamily. Cofactor: zinc. L7N6B3 No intermediary metabolism and respiration Q7DAC8 L7N6B3 GO:0055114,GO:0008270,GO:0004022 1.1.1.1 L7N6B3 NC_000962.3 Mycobrowser_v5 CDS 710584 710850 . - 0 Rv0616c Rv0616c Unknown Hypothetical protein Rv0616c, (MTCY19H5.05), len: 88 aa. Hypothetical unknown protein. O07761 No conserved hypotheticals O07761 NC_000962.3 Mycobrowser_v5 CDS 193117 193572 . + 0 Rv0163 Rv0163 Function unknown Conserved protein Rv0163, (MTCI28.03), len: 151 aa. Conserved protein, similar to others e.g. Q44017 hypothetical 16.6 KDA protein in GBD 5'region (ORF6)from Alcaligenes eutrophus (145 aa), FASTA scores: opt: 155, E(): 0.0002, (26.6% identity in 139 aa overlap). Also weak similarity with MTV008.31c|Rv2475c|B70867 from Mycobacterium tuberculosis (138 aa). O07408 No conserved hypotheticals O07408 O07408 O07408 NC_000962.3 Mycobrowser_v5 CDS 193626 194111 . + 0 Rv0164 TB18.5 Function unknown Conserved protein TB18.5 Rv0164, (MTCI28.04), len: 161 aa. TB18.5, conserved protein, equivalent to CAB08818.1|Z95398 hypothetical protein from Mycobacterium leprae (156 aa) FASTA scores: opt: 762, E(): 0, (76.3% identity in 152 aa overlap). Some similarity to Rv2185c, Rv0854, Rv0857 from Mycobacterium tuberculosis. Alternative start codon has been suggested. 3' part corrected since first submission (-24 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). L7N657 No conserved hypotheticals Q8VKQ8 L7N657 L7N657 NC_000962.3 Mycobrowser_v5 CDS 194144 194815 . - 0 Rv0165c mce1R Involved in transcriptional mechanism Probable transcriptional regulatory protein Mce1R (probably GntR-family) Rv0165c, (MTCI28.05c), len: 223 aa. Probable mce1R, transcriptional regulator, GntR family (See Casali et al., 2006), showing some similarity to several e.g. NTRA_CHELE|P54988 nta operon transcriptional regulator (231 aa), FASTA scores: opt: 154, E(): 0.00058, (32.0% identity in 125 aa overlap); P46833|GNTR_BACLI gluconate operon transcriptional repressor from Bacillus licheniformis (243 aa); GNTR_BACSU gluconate operon repressor from Bacillus subtilis (243 aa). Also similar to Rv0043c from Mycobacterium tuberculosis. Seems to belong to the GntR family of transcriptional regulators. Start changed since first submission (-41 aa). Q79G00 No regulatory proteins Q79G00 Q79G00 GO:0006355,GO:0006350,GO:0003700,GO:0005622 Q79G00 NC_000962.3 Mycobrowser_v5 CDS 197660 198529 . + 0 Rv0168 yrbE1B Unknown Conserved integral membrane protein YrbE1B Rv0168, (MTCI28.08), len: 289 aa. YrbE1B, unknown integral membrane protein, part of mce1 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07790|Rv0588|MTCY19H5.34|yrbE2B (295 aa); O53966|Rv1965|MTV051.03|yrbE3B (271 aa); etc. Also highly similar to conserved hypothetical integral membrane proteins of the yrbEB type, e.g. NP_302655.1|NC_002677 conserved membrane protein from Mycobacterium leprae (289 aa); P45030|YRBE_HAEIN|HI1086 hypothetical protein from Haemophilus influenzae (261 aa), FASTA scores: opt: 223, E(): 7.6e-07, (23.7% identity in 257 aa overlap); etc. L0T2Q9 No virulence, detoxification, adaptation O07413 L0T2Q9 L0T2Q9 NC_000962.3 Mycobrowser_v5 CDS 475350 475742 . + 0 Rv0396 Rv0396 Unknown Hypothetical protein Rv0396, (MTCY04D9.08), len: 130 aa. Hypothetical unknown protein. P95204 No conserved hypotheticals P95204 NC_000962.3 Mycobrowser_v5 CDS 704752 705909 . - 0 Rv0610c Rv0610c Unknown Hypothetical protein Rv0610c, (MTCY19H5.11), len: 385 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6Y481 No conserved hypotheticals I6Y481 I6Y481 NC_000962.3 Mycobrowser_v5 CDS 1836387 1836830 . - 0 Rv1632c Rv1632c Unknown Hypothetical protein Rv1632c, (MTCY01B2.24c), len: 147 aa. Hypothetical unknown protein. O06149 No conserved hypotheticals O06149 O06149 O06149 NC_000962.3 Mycobrowser_v5 CDS 199895 200935 . + 0 Rv0170 mce1B Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce1B Rv0170, (MTCI28.10), len: 346 aa. Mce1B (alternate gene name: mceD); belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07788|Rv0590|MTCY19H5.32c|mce2B (275 aa); O53968|Rv1967|MTV051.05|mce3B (342 aa); etc. Also highly similar to others e.g. NP_302657.1|NC_002677 putative secreted protein from Mycobacterium leprae (346 aa); CAC12797.1|AL445327 putative secreted protein from Streptomyces coelicolor (354 aa); etc. Contains hydrophobic region in N-terminal 30 residues. In Escherichia coli, N-terminal part is functional and directs export of a leaderless beta-lactamase into the periplasm (see Chubb et al., 1998). Predicted to be an outer membrane protein (See Song et al., 2008). O07414 No virulence, detoxification, adaptation O07414 O07414 O07414 NC_000962.3 Mycobrowser_v5 CDS 214088 214927 . + 0 Rv0183 Rv0183 Function unknown; probably involved in cellular metabolism. Possible lysophospholipase Rv0183, (MTCI28.23), len: 279 aa. Possible lysophospholipase, similar to several (especially eukaryotic enzymes, weaker with Escherichia coli), e.g. U67963|HSU67963_1 Human lysophospholipase homolog from Homo sapiens (313 aa), FASTA scores: opt: 569, E(): 2.6e-29, (37.1% identity in 259 aa overlap); P07000|PLDB_ECOLI lysophospholipase L2 from Escherichia coli (165 aa), FASTA scores: opt: 219, E(): 0.00012. Start changed based on similarity to AE001997_8 from Deinococcus radiodurans (282 aa), FASTA scores: opt: 510, E(): 1.4e-25, (34.8% identity in 282 aa overlap). Also shows some similarity to epoxide hydrolases from Mycobacterium tuberculosis e.g. Rv1938 FASTA score: (30.7% identity in 114 aa overlap); and O07214|YR15_MYCTU|Rv2715|MT2788|MTCY05A6.36 (341 aa). O07427 No intermediary metabolism and respiration O07427 GO:0016787 3.1.-.- O07427 NC_000962.3 Mycobrowser_v5 CDS 213028 214140 . - 0 Rv0182c sigG The sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released. Probable alternative RNA polymerase sigma factor SigG (RNA polymerase ECF type sigma factor) Rv0182c, (MTCI28.22c), len: 370 aa (start site uncertain; first of several possibles was chosen, but note that this overlaps the upstream ORF). Probable sigG, alternative RNA polymerase sigma subunit (see citations below), similar to many e.g. Q45585|SIGW_BACSU RNA polymerase sigma factor from Bacillus subtilis (187 aa). Also similar to nine other ECF sigma factors from Mycobacterium tuberculosis e.g. Rv1221, Rv0735, etc. Contains PS01063 Sigma-70 factors ECF subfamily signature and probable helix-turn helix motif from aa 205-226 (Score 1181, +3.21 SD). Belongs to the sigma-70 factor family, ECF subfamily. P9WGG5 No information pathways O07426 P9WGG5 GO:0016987,GO:0003700,GO:0006352,GO:0006355 P9WGG5 NC_000962.3 Mycobrowser_v5 CDS 382490 382876 . + 0 Rv0313 Rv0313 Function unknown Conserved protein Rv0313, (MTCY63.18), len: 128 aa. Conserved protein, equivalent only to CAC32049.1|AL583926 conserved hypothetical protein from Mycobacterium leprae (130 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WL03 No conserved hypotheticals P9WL03 NC_000962.3 Mycobrowser_v5 CDS 438302 438739 . - 0 Rv0360c Rv0360c Function unknown Conserved protein Rv0360c, (MTCY13E10.22c), len: 145 aa. Conserved protein, equivalent to AL023514|MLCB4_16|CAA18948.1|AL023514|MLCB4.27c hypothetical protein from Mycobacterium leprae (137 aa), FASTA scores: opt: 793, E(): 0, (85.4% identity in 137 aa overlap). And similar to AL049754|SCH10_25c|T36537 hypothetical protein from Streptomyces coelicolor (143 aa), FASTA scores: opt: 497, E(): 3.2e-27, (55.8% identity in 138 aa overlap). O06310 No conserved hypotheticals O06310 O06310 NC_000962.3 Mycobrowser_v5 CDS 985513 985971 . - 0 Rv0887c Rv0887c Function unknown Conserved hypothetical protein Rv0887c, (MTCY31.15c), len: 152 aa. Conserved hypothetical protein, highly similar to others e.g. NP_436346.1|NC_003037 Hypothetical protein from Sinorhizobium meliloti (149 aa); AL132644|SCI8_26 hypothetical protein from Streptomyces coelicolor (194 aa), FASTA scores: opt: 220, E(): 1.5e-07, (33.6% identity in 131 aa overlap); etc. Also shows weak similarity with transposases and related proteins. P9WKQ3 No conserved hypotheticals P64741 P9WKQ3 P9WKQ3 NC_000962.3 Mycobrowser_v5 CDS 202476 204068 . + 0 Rv0172 mce1D Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce1D Rv0172, (MTCI28.12), len: 530 aa. Mce1D; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07786|Rv0592|MTCY19H5.30c|mce2D (508 aa); O53970|Rv1969|MTV051.07|mce3D (423 aa); etc. Also highly similar to others e.g. NP_302659.1|NC_002677 putative secreted protein from Mycobacterium leprae (531 aa); CAC12795.1|AL445327 putative secreted protein from Streptomyces coelicolor (337 aa); etc. Hydrophobic region at N-terminus. Predicted to be an outer membrane protein (See Song et al., 2008). O07416 No virulence, detoxification, adaptation O07416 O07416 O07416 NC_000962.3 Mycobrowser_v5 CDS 204065 205237 . + 0 Rv0173 lprK Unknown, but thought to be involved in host cell invasion. Possible Mce-family lipoprotein LprK (Mce-family lipoprotein Mce1E) Rv0173, (MTCI28.13), len: 390 aa. Possible lprK (alternate gene name: mce1E), lipoprotein which belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07785|LPRL|Rv0593|MTCY19H5.29|mce2E (402 aa); O53971|LPRM|Rv1970|MTV051.08|mce3E (377 aa); etc. Also highly similar to others e.g. NP_302660.1|NC_002677 putative lipoprotein from Mycobacterium leprae (392 aa); CAC12794.1|AL445327 putative secreted protein from Streptomyces coelicolor (413 aa); etc. Contains PS00013 prokaryotic membrane lipoprotein lipid attachment site. O07417 No cell wall and cell processes O07417 O07417 O07417 NC_000962.3 Mycobrowser_v5 CDS 207452 208420 . + 0 Rv0176 Rv0176 Unknown Probable conserved Mce associated transmembrane protein Rv0176, (MTCI28.16), len: 322 aa. Probable conserved Mce-associated transmembrane protein. Contains short region of similarity to PRA_MYCLE|P41484 proline-rich antigen (36 kDa antigen) from Mycobacterium leprae (249 aa) (outside the proline-rich region), FASTA scores: opt: 165, E(): 2.9e-05, (40.0% identity in 65 aa overlap). Also similar to mce-associated proteins from Mycobacterium tuberculosis e.g. Rv1363c, Rv0177, Rv3493c, etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O07420 No cell wall and cell processes O07420 O07420 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 221871 222161 . + 0 Rv0190 Rv0190 Function unknown Conserved protein Rv0190, (MTCI28.29), len: 96 aa. Conserved protein, highly similar to several hypothetical proteins e.g. SYCSLRA_35|Q55554|SLL0176 hypothetical 18.9 kDa protein from Synechocystis (167 aa), FASTA scores: opt: 237, E(): 5.8e-16, (39.4% identity in 94 aa overlap). Also highly similar to Z95398|MLCL622_7|O06070 from Mycobacterium leprae (135 aa), FASTA score: (82.6% identity in 92 aa overlap). Also similar to hypothetical proteins from Mycobacterium tuberculosis e.g. Rv0967, Rv0030, Rv1766 (42.5% identity in 80 aa overlap). O07434 No conserved hypotheticals O07434 O07434 O07434 NC_000962.3 Mycobrowser_v5 CDS 219486 219917 . + 0 Rv0188 Rv0188 Unknown Probable conserved transmembrane protein Rv0188, (MTCI28.27), len: 143 aa. Probable conserved transmembrane protein, similar to T35347|4835334|CAB42956.1|AL049863|SC5H1_31 probable membrane protein from Streptomyces coelicolor (147 aa), FASTA scores: opt: 326, E(): 6.5e-15, (36.2% identity in 141 aa overlap); N-terminus of P80185|MTRC_METTH tetrahydromethanopterin S-methyltransferase subunit C from Methanobacterium thermoautotrophicum strain Marburg/DSM 2133 (266 aa), FASTA scores: opt: 125, E(): 0.033, (31.6% identity in 98 aa overlap). Also similar to Rv3635 from Mycobacterium tuberculosis. O07432 No cell wall and cell processes O07432 O07432 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 286898 287071 . - 0 Rv0236A Rv0236A Unknown Small secreted protein Rv0236A, len: 57 aa. Small secreted protein. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P0A5C5 No cell wall and cell processes P0A5C5 P0A5C5 NC_000962.3 Mycobrowser_v5 CDS 206814 207455 . + 0 Rv0175 Rv0175 Unknown Probable conserved Mce associated membrane protein Rv0175, (MTCI28.15), len: 213 aa. Probable conserved Mce-associated membrane protein, equivalent, but longer in N-terminus, to CAC32127.1|AL583926 possible membrane protein from Mycobacterium leprae (182 aa). Also similar to mce-associated proteins from Mycobacterium tuberculosis e.g. Rv1363c, Rv0177, Rv1973, etc. Contains two 12 residue direct repeats at N-terminus. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O07419 No cell wall and cell processes O07419 O07419 NC_000962.3 Mycobrowser_v5 CDS 216269 218344 . + 0 Rv0186 bglS Possibly involved in degradation [catalytic activity: hydrolysis of terminal, non-reducing beta-D-glucose residues with release of beta-D-glucose]. Probable beta-glucosidase BglS (gentiobiase) (cellobiase) (beta-D-glucoside glucohydrolase) Rv0186, (MTCI28.25b), len: 691 aa. Probable bglS, beta-glucosidase, highly similar to many e.g. BGLS_AGRTU|P27034 beta-glucosidase from Agrobacterium tumefaciens (818 aa), FASTA scores: opt: 643, E(): 0, (32.5% identity in 842 aa overlap). Seems to belong to family 3 of glycosyl hydrolases. O07430 No intermediary metabolism and respiration O07430 O07430 GO:0005975,GO:0008422 3.2.1.21 O07430 NC_000962.3 Mycobrowser_v5 CDS 218390 218551 . - 0 Rv0186A mymT Coordinates Cu(I) ions into a Cu(I)-thiolate core, protects cell from copper toxicity Metallothionein, MymT Rv0186A, len: 53 aa. MymT, metallothionein, equivalent to MAV_4993|A0QMH5 hypothetical protein from Mycobacterium avium (strain 104) (51 aa), and MAP_3626c|Q73TU2 hypothetical protein from Mycobacterium avium subsp. paratuberculosis (51 aa), FASTA scores: opt: 312, E(): 4.6e-17, (81.2% identity in 48 aa overlap). P9WK09 No virulence, detoxification, adaptation P9WK09 P9WK09 NC_000962.3 Mycobrowser_v5 CDS 218705 219367 . + 0 Rv0187 Rv0187 Thought to be involved in transfer of methyl group. Probable O-methyltransferase Rv0187, (MTCI28.26), len: 220 aa. Probable O-methyltransferase, similar to many e.g. AB93458.1|AL357591 putative O-methyltransferase from Streptomyces coelicolor (223 aa); MDMC_STRMY|Q00719 O-methyltransferase from Streptomyces mycarofaciens (221 aa), FASTA scores: opt: 327, E(): 2.4e-17, (35.9% identity in 192 aa overlap). Also similar to Rv1703c, Rv1220c from Mycobacterium tuberculosis. O07431 No intermediary metabolism and respiration O07431 O07431 GO:0008171 2.1.1.- O07431 NC_000962.3 Mycobrowser_v5 CDS 265507 266295 . + 0 Rv0222 echA1 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Probable enoyl-CoA hydratase EchA1 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv0222, (MTCY08D5.17), len: 262 aa. Probable echA1, enoyl-CoA hydratase, similar to others e.g. AAC77915.1|AF063588 enoyl CoA hydratase from Rhodococcus fascians (275 aa); etc. P96404 No lipid metabolism P96404 P96404 GO:0016853,GO:0008152,GO:0004300 4.2.1.17 P96404 NC_000962.3 Mycobrowser_v5 CDS 328575 329708 . - 0 Rv0272c Rv0272c Unknown Unknown protein Rv0272c, (MTCY06A4.16c), len: 377 aa. Unknown protein. P95229 No conserved hypotheticals P95229 NC_000962.3 Mycobrowser_v5 CDS 382879 383541 . - 0 Rv0314c Rv0314c Unknown Possible conserved membrane protein Rv0314c, (MTCY63.19c), len: 220 aa. Possible conserved membrane protein, with hydrophobic stretch from residues ~75-100. Similar in C-terminal part to Mycobacterium tuberculosis proteins Rv0679c and Rv0680c. O07241 No cell wall and cell processes O07241 O07241 NC_000962.3 Mycobrowser_v5 CDS 705961 706344 . - 0 Rv0611c Rv0611c Unknown Hypothetical protein Rv0611c, (MTCY19H5.10), len: 127 aa. Hypothetical unknown protein. Note that first start has been taken although this overlaps slightly with the upstream ORF. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6XVR9 No conserved hypotheticals I6XVR9 NC_000962.3 Mycobrowser_v5 CDS 222289 223530 . + 0 Rv0191 Rv0191 Unknown; possibly involved in transport of drug across the membrane. Probable conserved integral membrane protein Rv0191, (MTCI28.30), len: 413 aa. Probable conserved integral membrane protein, member of major facilitator superfamily (MFS) possibly involved in transport of drug, similar to several hypothetical proteins e.g. YDEA_ECOLI|P31122 hypothetical 42.5 kd protein from Escherichia coli (396 aa), FASTA scores: opt: 475, E(): 4.2e-33, (29.7% identity in 381 aa overlap); and to several chloramphenicol resistance proteins e.g. CMLR_STRLI|P31141 chloramphenicol resistance protein from Streptomyces lividans (392 aa), FASTA scores: opt: 394, E(): 6.7e-12, (28.2% identity in 383 aa overlap). Also similar to SVU09991_1 from Mycobacterium tuberculosis. P9WJX7 No cell wall and cell processes O07435 P9WJX7 GO:0005886,GO:0055085,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 223564 224664 . + 0 Rv0192 Rv0192 Function unknown Conserved hypothetical protein Rv0192, (MTCI28.31), len: 366 aa. Conserved hypothetical protein. Has Gly- Arg-rich region followed by highly Pro-rich repetitive region near N-terminus. Similar in C-terminus to other hypothetical proteins e.g. Q49706|B1496_F2_81|U00013 from Mycobacterium leprae (271 aa), FASTA scores: opt: 375, E(): 3.2e-24, (36.1% identity in 255 aa overlap); YV09_MYCTU|Q11149|cY20G9.09 hypothetical 47.9 kDa protein from Mycobacterium tuberculosis (451 aa), FASTA scores: opt: 330, E(): 3.2e-13, (35.1% identity in 271 aa overlap). Also similar to Rv0116c, Rv1433, Rv2518c, Rv0483 from Mycobacterium tuberculosis. O07436 No conserved hypotheticals O07436 O07436 O07436 NC_000962.3 Mycobrowser_v5 CDS 226878 230462 . + 0 Rv0194 Rv0194 Thought to be involved in active transport of drugs across the membrane (export): multidrug resistance by an export mechanism. Responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane. Probable transmembrane multidrug efflux pump Rv0194, (MTV033.02), len: 1194 aa. Probable multidrug efflux pump (See Danilchanka et al., 2008), highly similar to many e.g. U62129|STU62129_2|T30293 ABC transport protein homolog from Salmonella typhi (1218 aa), FASTA scores: opt: 1116, E(): 0, (36.3% identity in 1209 aa overlap); CAB66302.1|AL136519 ABC transporter protein ATP-binding component from Streptomyces coelicolor (1243 aa); I84547 mdl protein from Escherichia coli (1143 aa); etc. Also similar to MTCY50_9 and MTCY50_10 from Mycobacterium tuberculosis, FASTA score: (33.8% identity in 574 aa overlap). Contains two PS00017 ATP/GTP-binding site motif A (P-loop) and one PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). Alternative start possible at 1823 but no RBS. O53645 No cell wall and cell processes O53645 O53645 GO:0042626,GO:0016021,GO:0055085,GO:0005524 O53645 NC_000962.3 Mycobrowser_v5 CDS 1078391 1078687 . + 0 Rv0968 Rv0968 Function unknown Conserved protein Rv0968, (MTCY10D7.06c), len: 98 aa. Conserved protein, part of cso operon, similar to NP_301579.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (92 aa). Also highly similar to conserved hypothetical proteins from Mycobacterium tuberculosis e.g. Rv3269 (93 aa), FASTA score: (51.1% identity in 94 aa overlap); and Rv1993c (90 aa). P9WKL9 No conserved hypotheticals P64779 P9WKL9 NC_000962.3 Mycobrowser_v5 CDS 3841714 3842076 . - 0 Rv3424c Rv3424c Unknown Hypothetical protein Rv3424c, (MTCY78.05), len: 120 aa. Hypothetical unknown protein. P9WKY3 No conserved hypotheticals P9WKY3 NC_000962.3 Mycobrowser_v5 CDS 231647 232231 . + 0 Rv0196 Rv0196 Possibly involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0196, (MTV033.04), len: 194 aa. Possible transcriptional regulatory protein, similar to two Bacillus subtilis regulators: P42105|YXAF_BACSU hypothetical 21.0 kDa protein (191 aa), FASTA scores: opt: 323, E(): 2.1e-15, (30.9% identity in 181 aa overlap); and Z99105|BSUB0002_9 negative regulator of the lincomycin operon (188 aa), FASTA scores: opt: 255, E(): 1e-10, (25.9 identity in 185 aa overlap). P9WME1 No regulatory proteins O53647 P9WME1 GO:0006350,GO:0003700,GO:0006355 P9WME1 NC_000962.3 Mycobrowser_v5 CDS 234516 236507 . - 0 Rv0198c zmp1 Function unknown; hydrolyzes peptides and/or proteins. Probable zinc metalloprotease Zmp1 Rv0198c, (MTV033.06c), len: 663 aa. Probable zmp1, zinc metalloprotease, equivalent to Z95398|MLCL622.12c from Mycobacterium leprae (667 aa), FASTA scores: opt: 3710, E(): 0, (80.8 % identity in 667 aa overlap). Also similar to many other metalloproteases e.g. members of the eukaryotic neprilysin family: P08473|NEP_HUMAN neprilysin (749 aa), FASTA scores: opt: 872, E(): 0, (31.1% identity in 692 aa overlap); Q07744|PEPO_LACLA neutral endopeptidase from Lactococcus lactis (626 aa), FASTA scores: opt: 862, E(): 0, (30.0% identity in 654 aa overlap). Contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. Belongs to peptidase family M13 (zinc metalloprotease); also known as the neprilysin subfamily. I6X8R2 No intermediary metabolism and respiration 3ZUK O53649 I6X8R2 GO:0006508,GO:0004222 3.4.24.- I6X8R2 NC_000962.3 Mycobrowser_v5 CDS 236550 237209 . + 0 Rv0199 Rv0199 Unknown Probable conserved membrane protein Rv0199, (MTV033.07), len: 219 aa. Probable conserved membrane protein, equivalent to Z95398|MLCL622.13 from Mycobacterium leprae (224 aa), FASTA scores: opt: 920, E(): 0, (67.7% identity in 220 aa overlap). Also some similarity to Mce-associated membrane proteins from Mycobacterium tuberculosis e.g. Rv0178, Rv0175, etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53650 No cell wall and cell processes O53650 O53650 NC_000962.3 Mycobrowser_v5 CDS 248115 248906 . - 0 Rv0208c Rv0208c Causes methylation Hypothetical methlytransferase (methylase) Rv0208c, (MTCY08D5.03c), len: 263 aa. Hypothetical methyltransferase, equivalent to Z95398|MLCL622_20 from Mycobacterium leprae (279 aa), FASTA score: (64.2% identity in 246 aa overlaps). Also similar to others e.g. 10178368|CAC08407.1|AL392177|Q9F305|MT04_STRCO|SCD17A.03c hypothetical methlytransferase from Streptomyces coelicolor (271 aa). Could start at aa 7. P9WFY9 No intermediary metabolism and respiration P67498 P9WFY9 GO:0006400,GO:0008176 2.1.1.33 P9WFY9 NC_000962.3 Mycobrowser_v5 CDS 254637 255950 . - 0 Rv0213c Rv0213c Causes methylation Possible methyltransferase (methylase) Rv0213c, (MTCY08D5.08c), len: 437 aa. Possible methyltransferase, weakly similar to others methyltransferases e.g. AF127374_30|LINA from Streptomyces lavendulae (611 aa), FASTA scores: opt: 400, E(): 8.1e-19, (27.3% identity in 388 aa overlap); Q50258 fortimicin kl1 methyltransferase (553 aa), FASTA scores: opt: 267, E(): 1.2e-13, (29.3% identity in 351 aa overlap). P96395 No intermediary metabolism and respiration P96395 P96395 GO:0009451,GO:0031419,GO:0046872,GO:0008168,GO:0051539 2.1.1.- P96395 NC_000962.3 Mycobrowser_v5 CDS 2204212 2204706 . + 0 Rv1961 Rv1961 Unknown Hypothetical protein Rv1961, MTCY09F9.03c, len: 164 aa. Hypothetical unknown protein. P95253 No conserved hypotheticals P95253 NC_000962.3 Mycobrowser_v5 CDS 241514 241924 . + 0 Rv0203 Rv0203 Unknown Possible exported protein Rv0203, (MTV033.11), len: 136 aa. Possible exported protein (has hydrophobic stretch near N-terminus). Some similarity to part of U02459|LDU02459_1 hypothetical protein from Leishmania donovani (741 aa), FASTA score: opt: 111, E(): 9.1, (30.0% identity in 90 aa overlap). I6X8R5 No cell wall and cell processes 3MAY I6X8R5 I6X8R5 NC_000962.3 Mycobrowser_v5 CDS 241976 243214 . - 0 Rv0204c Rv0204c Unknown Probable conserved transmembrane protein Rv0204c, (MTV033.12c), len: 412 aa. Probable conserved transmembrane protein (see citation below), equivalent, but has C-terminal extension, to Z95398|MLCL622.17c from Mycobacterium leprae (367 aa), FASTA scores: opt: 2002, E(): 0, (82.4% identity in 374 aa overlap). Some similarity to Rv0585c from Mycobacterium tuberculosis. Nucleotide position 242299 in the genome sequence has been corrected, C:G resulting in V306L. I6Y748 No cell wall and cell processes I6Y748 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 243384 244487 . + 0 Rv0205 Rv0205 Unknown Probable conserved transmembrane protein Rv0205, (MTV033.13), len: 367 aa. Possible conserved transmembrane protein, similar to hypothetical proteins from many bacteria e.g. AL0209|SC4H8_6 from Streptomyces coelicolor (402 aa), FASTA scores: opt: 436, E(): 1.7e-21, (27.2% identity in 349 aa overlap); Z99117|BSUB0014_221 from Bacillus subtilis (353 aa), FASTA scores: opt: 394, E(): 8.6e-19, (28.7% identity in 324 aa overla). P9WFM5 No cell wall and cell processes O53656 P9WFM5 GO:0005886,GO:0016021 P9WFM5 NC_000962.3 Mycobrowser_v5 CDS 244484 247318 . - 0 Rv0206c mmpL3 Unknown. Thought to be involved in fatty acid transport. Possible conserved transmembrane transport protein MmpL3 Rv0206c, (MTV033.14c, MTCY08D5.01c), len: 944 aa. Possible mmpL3, conserved transmembrane transport protein (see Tekaia et al., 1999), equivalent to Z95398|MLCL622.18c from Mycobacterium leprae (955 aa), FASTA scores: opt: 806, E(): 1.8e-21, (57.2% identity in 243 aa overlap). Member of RND superfamily, similar to others. Belongs to the MmpL family. P9WJV5 No cell wall and cell processes O53657 P9WJV5 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 247384 248112 . - 0 Rv0207c Rv0207c Function unknown Conserved hypothetical protein Rv0207c, (MTCY08D5.02c), len: 242 aa. Conserved hypothetical protein, equivalent to Z95398|MLCL622_19 from Mycobacterium leprae (261 aa), FASTA scores: E(): 0, (60.8 identity in 199 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P96389 No conserved hypotheticals P96389 P96389 NC_000962.3 Mycobrowser_v5 CDS 1003957 1004484 . + 0 Rv0901 Rv0901 Function unknown Possible conserved exported or membrane protein Rv0901, (MTCY31.29), len: 175 aa. Possible conserved exported or membrane protein, with hydrophobic N-terminus at aa 7-25. Shows some similarity in C-terminus to O33070|Z99494|MLCB57.59 hypothetical protein from Mycobacterium leprae (113 aa), FASTA scores: opt: 204, E(): 3.2e-12, (44.9% identity in 78 aa overlap). P9WJG5 No cell wall and cell processes P9WJG5 NC_000962.3 Mycobrowser_v5 CDS 3195545 3196432 . - 0 Rv2886c Rv2886c Prevents the cointegration of foreign DNA before integration into the chromosome. Probable resolvase Rv2886c, (MTCY274.17c), len: 295 aa. Probable resolvase for IS1539. Contains PS00213 Lipocalin signature. P9WL35 No insertion seqs and phages P65045 P9WL35 GO:0006310,GO:0000150,GO:0003677 P9WL35 NC_000962.3 Mycobrowser_v5 CDS 253669 254640 . - 0 Rv0212c nadR Involved in transcriptional mechanism. Possible transcriptional regulatory protein NadR (probably AsnC-family) Rv0212c, (MTCY08D5.07c), len: 323 aa. Possible nadR (alternate gene name: nadI), transcriptional regulator, similar to others e.g. NADR_ECOLI|P27278 transcriptional regulator from Escherichia coli (410 aa), FASTA scores: opt: 377, E (): 1e-17, (31.1% identity in 347 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). P96394 No regulatory proteins P96394 GO:0003824,GO:0019363,GO:0045449,GO:0030528,GO:0006810,GO:0003677 P96394 NC_000962.3 Mycobrowser_v5 CDS 258913 259926 . + 0 Rv0216 Rv0216 Unknown Double hotdog hydratase Rv0216, (MTCY08D5.11), len: 337 aa. Double hotdog R-specific hydratase of unknown function, shows no activity for crotonyl-CoA, equivalent to Z95398|MLCL622_22 from Mycobacterium leprae (339 aa), FASTA scores: E(): 0, (73.7 identity in 338 aa overlap). Shows structural similarity to six others in Mycobacterium tuberculosis (see Castell et al (2005) below). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). I6Y340 No intermediary metabolism and respiration 2BI0 I6Y340 I6Y340 NC_000962.3 Mycobrowser_v5 CDS 259923 260831 . - 0 Rv0217c lipW Function unknown; lipolytic enzyme probably involved in cellular metabolism. Possible esterase LipW Rv0217c, (MTCY08D5.12c), len: 302 aa. Possible esterase, showing similarity with others e.g. EST_ACICA|P18773 esterase (303 aa), FASTA scores: opt: 320, E(): 3.2e-13, (29.2% identity in 274 aa overlap). P96399 No intermediary metabolism and respiration P96399 P96399 GO:0016787 3.1.1.- P96399 NC_000962.3 Mycobrowser_v5 CDS 260924 262252 . + 0 Rv0218 Rv0218 Unknown Probable conserved transmembrane protein Rv0218, (MTCY08D5.13), len: 442 aa. Probable conserved transmembrane protein, some similarity with sulfite oxidases e.g. SUOX_HUMAN|P51687 sulfite oxidase precursor (488 aa), FASTA scores: opt: 153, E(): 0.0087, (28.6% identity in 161 aa overlap); and with some nitrate reductases e.g. NIA_FUSOX|P39863 nitrate reductase (NADPH) (905 aa), FASTA scores: opt: 143, E(): 0.06, (29.3% identity in 92 aa overlap). Also similar to BSUB0017_86 from Mycobacterium tuberculosis. P96400 No cell wall and cell processes P96400 P96400 GO:0016021,GO:0009055 P96400 NC_000962.3 Mycobrowser_v5 CDS 387888 388550 . + 0 Rv0320 Rv0320 Unknown Possible conserved exported protein Rv0320, (MTCY63.25), len: 220 aa. Possible conserved exported protein, similar to some hypothetical proteins and to the middle part of a peptidase: NP_066789.1|10657900|AAG21739.1|AF116907 putative peptidase from Rhodococcus equi (546 aa). Also similar to Rv1728c|MTCY04C12.13c from Mycobacterium tuberculosis (256 aa), FASTA scores: opt: 497, E(): 1.2e-26, (41.8% identity in 225 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). O07246 No cell wall and cell processes O07246 O07246 NC_000962.3 Mycobrowser_v5 CDS 264067 265476 . + 0 Rv0221 Rv0221 May be involved in synthesis of triacylglycerol Possible triacylglycerol synthase (diacylglycerol acyltransferase) Rv0221, (MTCY08D5.16), len: 469 aa. Possible triacylglycerol synthase (See Daniel et al., 2004), similar to other proteins from Mycobacterium tuberculosis e.g. Q50680|Rv2285|MT2343|MTCY339.25c 47.7 kDa protein (445 aa), FASTA scores: opt: 455, E(): 8.1e-23, (26.7% identity in 461 aa overlap); Rv3740c, Rv3734c, etc. P9WKB7 No lipid metabolism P96403 P9WKB7 GO:0006071,GO:0008610,GO:0004144 2.3.1.20 NC_000962.3 Mycobrowser_v5 CDS 267863 268627 . - 0 Rv0224c Rv0224c Causes methylation Possible methyltransferase (methylase) Rv0224c, (MTCY08D5.19c), len: 254 aa. Possible methyltransferase, showing weak similarity with other methyltransferases e.g. P74388 sterol-C-methyltransferase (318 aa), FASTA scores: opt: 190, E(): 3.6e-05, (33.3% identity in 114 aa overlap). Equivalent to AL022486|MLCB1883_1 from Mycobacterium leprae (269 aa), FASTA scores: opt: 1456, E(): 0, (82.9% identity in 252 aa overlap). Also some similarity with MTCY21B4.22c from Mycobacterium tuberculosis FASTA score: (30.1% identity in 136 aa overlap). P9WJZ9 No intermediary metabolism and respiration P96406 P9WJZ9 GO:0008168,GO:0008152 2.1.1.- P9WJZ9 NC_000962.3 Mycobrowser_v5 CDS 605604 606152 . + 0 Rv0513 Rv0513 Unknown Possible conserved transmembrane protein Rv0513, (MTCY20G10.03), len: 182 aa. Possible conserved transmembrane protein, with its N-terminus highly similar to S72925|B2168_C1_182 hypothetical protein from Mycobacterium leprae (103 aa), FASTA scores: opt: 217, E(): 8.2e-14, (45.3 % identity in 106 aa overlap). O33358 No cell wall and cell processes O33358 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 268663 269817 . + 0 Rv0225 Rv0225 Possibly involved in LPS biosynthesis. Possible conserved protein Rv0225, (MTCY08D5.20), len: 384 aa. Possible conserved protein involved in LPS biosynthesis, similar to O26275 LPS biosynthesis RFBU related protein (382 aa), FASTA scores: opt: 426, E(): 1.2e-20, (28.2% identity in 394 aa overlap). Some similarity with Rv3032 from Mycobacterium tuberculosis FASTA score: (31.6% identity in 228 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). P96407 No cell wall and cell processes P96407 P96407 GO:0016740,GO:0009058 P96407 NC_000962.3 Mycobrowser_v5 CDS 296809 298119 . + 0 Rv0246 Rv0246 Unknown Probable conserved integral membrane protein Rv0246, (MTV034.12), len: 436 aa (start uncertain). Probable conserved integral membrane protein, similar to Rv2209|1237062|CAA94252.1|Z70283|Q10398|YM09_MYCTU from Mycobacterium tuberculosis (512 aa), FASTA scores: opt: 712, E(): 0, (33.2% identity in 422 aa overlap). O53668 No cell wall and cell processes O53668 NC_000962.3 Mycobrowser_v5 CDS 349935 351476 . + 0 Rv0286 PPE4 Function unknown PPE family protein PPE4 Rv0286, (MTV035.14), len: 513 aa. PPE4, Member of the Mycobacterium tuberculosis PPE family, similar to others e.g. AL0212|MTV012_32 from Mycobacterium tuberculosis (434 aa), FASTA scores: opt: 958, E(): 0, (43.5% identity in 522 aa overlap). P9WI43 No PE/PPE Q7DA35 P9WI43 GO:0005886,GO:0016021 P9WI43 NC_000962.3 Mycobrowser_v5 CDS 554016 554309 . + 0 Rv0463 Rv0463 Unknown Probable conserved membrane protein Rv0463, (MTV038.07), len: 97 aa. Probable conserved transmembrane protein, highly similar to AAA63017.1|U15183 hypothetical protein from Mycobacterium leprae (101 aa), FASTA scores: opt: 364, E(): 4e-21, (57.9% identity in 95 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53748 No cell wall and cell processes O53748 NC_000962.3 Mycobrowser_v5 CDS 271574 272839 . - 0 Rv0227c Rv0227c Unknown Probable conserved membrane protein Rv0227c, (MTCY08D5.22c), len: 421 aa. Possible conserved membrane protein, equivalent to AL022486|MLCB1883_4 from Mycobacterium leprae (448 aa), FASTA scores: opt: 2148, E(): 0, (76.6% identity in 423 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P96409 No cell wall and cell processes P96409 P96409 P96409 NC_000962.3 Mycobrowser_v5 CDS 273055 274278 . + 0 Rv0228 Rv0228 Function unknown; probably involved in cellular metabolism. Probable integral membrane acyltransferase Rv0228, (MTCY08D5.23), len: 407 aa. Probable integral membrane acyltransferase, equivalent to 3063875|CAA18555.1|AL022486|T44870 acyltransferase from Mycobacterium leprae (384 aa), FASTA scores: opt: 2004, E(): 0, (79.3% identity in 381 aa overlap). Also similar to others e.g. Q11064 probable acyltransferase CY50.28C (383 aa), FASTA scores: opt: 372, E(): 2.6e-16, (35.9% identity in 359 aa overlap); Q00718|MDMB_STRMY acyltransferase. Very similar to Rv0111, Rv1254, etc from Mycobacterium tuberculosis. P96410 No intermediary metabolism and respiration P96410 P96410 GO:0016020,GO:0008415 2.3.1.- NC_000962.3 Mycobrowser_v5 CDS 274306 274986 . - 0 Rv0229c Rv0229c Unknown Possible conserved membrane protein with PIN domain Rv0229c, (MTCY08D5.24c), len: 226 aa. Possible conserved membrane protein with PIN domain in C-terminal half, similar to several others from Mycobacterium tuberculosis. Has some similarity with Rv2757c|D70880 from Mycobacterium tuberculosis (138 aa). (See Arcus et al., 2005). FASTA scores: E(): 1e-15, (45.3% identity in 137 aa overlap), and Rv0301, Rv2546, etc. Also some similarity with Q48177 virulence associated protein C (132 aa), FASTA scores: opt: 101, E(): 0.6, (24.3% identity in 136 aa overlap). Contains PS00626 Regulator of chromosome condensation (RCC1) signature 2. L0T5V6 No cell wall and cell processes P96411 L0T5V6 L0T5V6 NC_000962.3 Mycobrowser_v5 CDS 305453 305809 . + 0 Rv0253 nirD Involved in nitrate assimilation (denitrification); required for activity of the reductase [catalytic activity: 3 NAD(P)H + nitrite = 3 NAD(P)+ + NH4OH + H2O.] Probable nitrite reductase [NAD(P)H] small subunit NirD Rv0253, (MTV034.19), len: 118 aa. Probable nirD, nitrite reductase [NAD(P)H] small subunit, similar to others e.g. P23675|NIRD_ECOLI|B3366|Z4727|ECS4217 from Escherichia coli strains K12 and O157:H7 (108 aa), FASTA scores: opt: 271, E():1.7e-12, (41.9% identity in 105 aa overlap). Associates with NIRB|Rv0252. O53675 No intermediary metabolism and respiration O53675 GO:0042128,GO:0008942,GO:0055114,GO:0051537 1.6.6.4 O53675 NC_000962.3 Mycobrowser_v5 CDS 718025 718285 . + 0 Rv0626 vapB5 Possible ribonuclease Possible antitoxin VapB5 Rv0626, (MTCY20H10.07), len: 86 aa. Possible vapB5, antitoxin, part of toxin-antitoxin (TA) operon with Rv0627 (See Arcus et al., 2005; Pandey and Gerdes, 2005)., similar to others in Mycobacterium tuberculosis hypothetical proteins e.g. Rv0596c, Rv3385c, Rv3407,Rv3181c, etc. Cofactor: Mg2+ P9WF19 No virulence, detoxification, adaptation 3DBO P96916 P9WF19 P9WF19 NC_000962.3 Mycobrowser_v5 CDS 274983 275963 . - 0 Rv0230c php Enzymatic activity unknown [catalytic activity: aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol]. Probable phosphotriesterase Php (parathion hydrolase) (PTE) (aryldialkylphosphatase) (paraoxonase) (a-esterase) (aryltriphosphatase) (paraoxon hydrolase) Rv0230c, (MTCY08D5.26c), len: 326 aa. Probable php, phosphotriesterase, similar to others e.g. AAK42653.1|AE006849 putative aryldialkylphosphatase (phosphotriesterase) (paraoxonase) from Sulfolobus solfataricus (314 aa); PHP_ECOLI|P45548 phosphotriesterase homology protein from Escherichia coli (292 aa), FASTA scores: opt: 408, E(): 7.1e-20, (31.1% identity in 305 aa overlap); OPD_FLASP|P16648 parathion hydrolase precursor (365 aa), FASTA scores: opt: 319, E(): 5.1e-14, (34.5% identity in 333 aa overlap); etc. Belongs to the phosphotriesterase family. Cofactor: contains 2 moles of zinc per subunit. P9WHN9 No lipid metabolism P96413 P9WHN9 GO:0016788,GO:0008270,GO:0009056 P9WHN9 NC_000962.3 Mycobrowser_v5 CDS 289104 289337 . + 0 Rv0239 vapB24 Unknown Possible antitoxin VapB24 Rv0239, (MTV034.05), len: 77 aa. Possible vapB24, antitoxin, part of toxin-antitoxin (TA) operon with Rv0240. Weakly similar to others e.g. Rv1839c|Z83859|MTCY359_34 from Mycobacterium tuberculosis (87 aa). See Arcus et al. 2005. FASTA scores: opt: 88, E(): 5, (40.0% identity in 45 aa overlap). P9WJ41 No virulence, detoxification, adaptation P9WJ41 NC_000962.3 Mycobrowser_v5 CDS 479789 480160 . + 0 Rv0401 Rv0401 Unknown Probable conserved transmembrane protein Rv0401, (MTCY04D9.14), len: 123 aa. Probable conserved transmembrane protein, equivalent to AL023514|MLCB4_9 putative integral membrane protein from Mycobacterium leprae (122 aa), FASTA scores: opt: 548, E(): 4.4e-32, (66.9% identity in 121 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P95210 No cell wall and cell processes P95210 P95210 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1241115 1241390 . - 0 Rv1116A Rv1116A Function unknown Conserved hypothetical protein (fragment) Rv1116A, len: 91 aa. Conserved hypothetical protein (possibly gene fragment), similar to C-terminal part of Rv1646|Z85982_9 from Mycobacterium tuberculosis (310 aa), FASTA scores: opt: 301, E(): 9.3e-13, (68.05% identity in 72 aa overlap). Also overlaps gene on other strand, Rv1116, at 3'-end. L7N6A9 No conserved hypotheticals L7N6A9 L7N6A9 NC_000962.3 Mycobrowser_v5 CDS 277899 278588 . + 0 Rv0232 Rv0232 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably TetR/AcrR-family) Rv0232, (MTCY08D5.28), len: 229 aa. Probable transcriptional regulatory protein, TetR/AcrR family, similar to others e.g. YIXD_BACSU|P32398 hypothetical transcriptional regulator (191 aa), FASTA scores: opt: 149, E(): 0.0014, (21.5% identity in 158 aa overlap). Also similar to MTV030_11 from Mycobacterium tuberculosis. Contains PS01081 Bacterial regulatory proteins, TetR family signature, and probable helix-turn helix motif from aa 33-54 (Score 1142, +3.08 SD). Belongs to the TetR/AcrR family of transcriptional regulators. P96415 No regulatory proteins P96415 P96415 GO:0006350,GO:0003700,GO:0006355 P96415 NC_000962.3 Mycobrowser_v5 CDS 278585 279529 . + 0 Rv0233 nrdB Involved in the DNA replication pathway (first reaction). Provides the precursors necessary for DNA synthesis [catalytic activity: 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O = ribonucleoside diphosphate + reduced thioredoxin]. Ribonucleoside-diphosphate reductase (beta chain) NrdB (ribonucleotide reductase small chain) Rv0233, (MTCY08D5.29), len: 314 aa. nrdB (alternate gene name: rnrS) ribonucleoside-diphosphate reductase, beta chain, similar to others e.g. RIR2_SCHPO|P36603 ribonucleoside-diphosphate reductase (391 aa), FASTA scores: opt: 168, E(): 0.00018, (26.1% identity in 199 aa overlap); etc. Belongs to the ribonucleoside diphosphate reductase small chain family. Cofactor: iron, manganese P9WH69 No information pathways 3EE4 P96416 P9WH69 GO:0055114,GO:0004748,GO:0046914,GO:0009186 1.-.-.- P9WH69 NC_000962.3 Mycobrowser_v5 CDS 281166 282614 . - 0 Rv0235c Rv0235c Unknown Probable conserved transmembrane protein Rv0235c, (MTCY08D5.31c), len: 482 aa. Probable conserved transmembrane protein, highly similar to AL133278|CAB61913.1|SCM11_2 putative integral membrane protein from Streptomyces coelicolor (470 aa), FASTA scores: opt: 2116, E(): 0, (61.8% identity in 474 aa overlap); and similar to hypothetical proteins from other organisms e.g. Q13392|384D8_7 hypothetical protein (579 aa), FASTA scores: opt: 355, E(): 6.9e-17, (28.5% identity in 569 aa overlap). P96418 No cell wall and cell processes P96418 P96418 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 282649 286851 . - 0 Rv0236c aftD Involved in the biosynthesis of the mycobacterial cell wall arabinan Possible arabinofuranosyltransferase AftD Rv0236c, (MTV034.01c, MTV034.02c, MTCY08D5.32c), len: 1400 aa. Possible aftD, arabinofuranosyltransferase (See Skovierova et al., 2009). Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). Probable conserved transmembrane protein, equivalent to AL022486|CAC32102.1|MLCB1883_7 possible integral membrane protein from Mycobacterium leprae (1440 aa), FASTA scores: opt: 7491, E(): 0, (78.8% identity in 1397 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P96419 No cell wall and cell processes P96419 P96419 GO:0016021,GO:0007155 P96419 NC_000962.3 Mycobrowser_v5 CDS 288428 289042 . + 0 Rv0238 Rv0238 Possibly involved in transcriptional mechanism. Possible transcriptional regulatory protein (probably TetR-family) Rv0238, (MTV034.04), len: 204 aa. Possible transcriptional regulatory protein, TetR family, equivalent to AL022486|MLCB1883_9|T44874 probable transcription regulator from Mycobacterium leprae (208 aa), FASTA scores: opt: 1029, E(): 0, (80.9% identity in 199 aa overlap). Also similar to others e.g. CAB77290.1|AL160312 putative TetR-family regulatory protein from Streptomyces coelicolor (240 aa). Also similar to Mycobacterium tuberculosis proteins Z95120|Rv3208 (228 aa), FASTA scores: opt: 266, E(): 8.3e-12, (28.1% identity in 196 aa overlap); and Rv1019 (197 aa). O53661 No regulatory proteins O53661 O53661 GO:0006350,GO:0003700,GO:0006355 O53661 NC_000962.3 Mycobrowser_v5 CDS 289812 290654 . - 0 Rv0241c htdX Involved in fatty acid synthesis type II (fas-II) Probable 3-hydroxyacyl-thioester dehydratase HtdX Rv0241c, (MTV034.07c), len: 280 aa. Probable htdX, 3-hydroxyacyl-thioester dehydratase (See Gurvitz et al., 2009), highly similar to MLCB1883.17c|T44876063881|CAA18566.1|AL022486 hypothetical protein from Mycobacterium leprae (280 aa), FASTA scores: opt: 1564, E(): 0, (81.8% identity in 280 aa overlap); and CAC32097.1|AL583926 conserved hypothetical protein from Mycobacterium leprae (300 aa). Shows structural similarity to six others in Mycobacterium tuberculosis (see Castell et al (2005) below). Also similar to proteins from other organisms e.g. CAB77291.1|AL160312 putative dehydratase from Streptomyces coelicolor (291 aa); part of BAA92930.1|AB032743 fatty acid synthetase beta subunit from Pichia angusta (2060 aa). Predicted to be an outer membrane protein (See Song et al., 2008). O53664 No intermediary metabolism and respiration O53664 O53664 GO:0004312,GO:0005835,GO:0055114,GO:0016491,GO:0006633 O53664 NC_000962.3 Mycobrowser_v5 CDS 302866 305427 . + 0 Rv0252 nirB Involved in nitrate assimilation (denitrification) (at the second step). This enzyme is a FAD flavoprotein that also contains a siroheme and one 2FE-2S iron-sulfur center [catalytic activity: 3 NAD(P)H + nitrite = 3 NAD(P)+ + NH4OH + H2O.] Probable nitrite reductase [NAD(P)H] large subunit [FAD flavoprotein] NirB Rv0252, (MTV034.18), len: 853 aa. Probable nirB (alternate gene name: nasB), nitrite reductase [NAD(P)H] large subunit, flavoprotein containing siroheme and a 2FE-2S iron-sulfur centre. Highly similar to many others bacterial enzymes e.g. P08201|NIRB_ECOLI nitrite reductase (NAD(P)H) large subunit from Escherichia coli strain K12 (847 aa), FASTA scores: opt: 2775, E(): 0, (49.8% identity in 840 aa overlap); Q06458|NIRB_KLEPN nitrite reductase (NAD(P)H) large subunit (957 aa), FASTA scores: opt: 2902, E(): 0, (54.2% identity in 827 aa overlap). Contains PS00365 Nitrite and sulfite reductases iron-sulfur/siroheme-binding site. Homodimer which associates with NIRD|Rv0253. Cofactors: FAD; Iron; Siroheme. O53674 No intermediary metabolism and respiration O53674 O53674 GO:0020037,GO:0050660,GO:0050661,GO:0042128,GO:0008942,GO:0055114,GO:0051539 1.6.6.4 O53674 NC_000962.3 Mycobrowser_v5 CDS 310774 311517 . - 0 Rv0259c Rv0259c Function unknown Conserved hypothetical protein Rv0259c, (MTCY06A4.03c), len: 247 aa. Conserved hypothetical protein, showing some similarity to Rv2393|Z81368|MTCY253_28 from Mycobacterium tuberculosis (281 aa), FASTA scores: E(): 9.5e-16, (33.6 % identity in 235 aa overlap). Also some similarity with CAC33938.1|AL589708 putative secreted protein from Streptomyces coelicolor (248 aa). P95216 No conserved hypotheticals P95216 P95216 GO:0016829,GO:0046872,GO:0009236 P95216 NC_000962.3 Mycobrowser_v5 CDS 339364 340974 . + 0 Rv0280 PPE3 Function unknown PPE family protein PPE3 Rv0280, (MTV035.08), len: 536 aa. PPE3, Member of the Mycobacterium tuberculosis PPE family, similar to others e.g. Z80108|MTCY21B4_4|Rv0453 from Mycobacterium tuberculosis (539 aa), FASTA scores: opt: 1131, E(): 0, (51.7% identity in 540 aa overlap). P9WI45 No PE/PPE Q79FY8 P9WI45 P9WI45 NC_000962.3 Mycobrowser_v5 CDS 351525 351818 . + 0 Rv0287 esxG Function unknown ESAT-6 like protein EsxG (conserved protein TB9.8) Rv0287, (MTV035.15), len: 97 aa. EsxG, ESAT-6 like protein. PE-family related protein; distant member of the Mycobacterium tuberculosis PE family, similar to Rv3020c|AL0212|MTV012.34 (97 aa), FASTA scores: opt: 564, E(): 0, (91.8% identity in 97 aa overlap). Contains probable helix-turn-helix motif at aa 14-35 (Score 144, +4.11 SD). Seems to belong to the ESAT6 family (see Gey Van Pittius et al., 2001). Note that previously known as TB9.8. O53692 No cell wall and cell processes 2KG7 O53692 O53692 NC_000962.3 Mycobrowser_v5 CDS 2544289 2544621 . - 0 Rv2269c Rv2269c Unknown Hypothetical protein Rv2269c, (MTCY339.41), len: 110 aa. Unknown protein; questionable ORF. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLF9 No conserved hypotheticals P9WLF9 P9WLF9 NC_000962.3 Mycobrowser_v5 CDS 314309 314854 . - 0 Rv0262c aac Confers resistance to aminoglycosides (gentamicin, tobramycin, dibekacin, netilmicin, and 6'-N-ethylnetilmicin). Aminoglycoside 2'-N-acetyltransferase Aac (Aac(2')-IC) Rv0262c, (MTCY06A4.06c), len: 181 aa. Aac, aminoglycoside 2'-N-acetyltransferase (aac(2')-IC) (see citation below), highly similar to NP_302635.1|NC_002677 aminoglycoside 2'-N-acetyltransferase from Mycobacterium leprae (182 aa); Q49157|AAC2_MYCFO|AAC aminoglycoside 2'-N-acetyltransferase from Mycobacterium fortuitum (195 aa), Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. FASTA scores: opt: 884, E(): 0, (69.1% identity in 181 aa overlap); and P94968|AAC2_MYCSM|AAC aminoglycoside 2'-N-acetyltransferase from Mycobacterium smegmatis (210 aa) (see also citation below). Also similar to Q52424|AAC2_PROST aminoglycoside 2'-N-acetyltransferase from Providencia stuartii (178 aa). Belongs to the AAC(2')-I family of acetyltransferases. Note that previously known as aac(2')-IC. P9WQG9 No virulence, detoxification, adaptation 1M44,1M4D,1M4G,1M4I P0A5N0 P9WQG9 GO:0008152,GO:0046677,GO:0008080 2.3.1.- P9WQG9 NC_000962.3 Mycobrowser_v5 CDS 292171 293493 . + 0 Rv0243 fadA2 Function unknown, but involved in lipid degradation [catalytic activity: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA]. Probable acetyl-CoA acyltransferase FadA2 (3-ketoacyl-CoA thiolase) (beta-ketothiolase) Rv0243, (MTV034.09), len: 440 aa. Probable fadA2, acetyl-CoA acyltransferase (3-acyl-CoA thiolase), equivalent, but shorter 17 aa, to AL022486|MLCB1883_14T44879 acetyltransferase from Mycobacterium leprae (457 aa), FASTA scores: opt: 250 7, E(): 0, (87.6% identity in 435 aa overlap). Also highly similar to many e.g. G83046|PA478 probable acyl-CoA thiolase from Pseudomonas aeruginosa (425 aa); AB77293.1|AL160312 putative ketoacyl CoA thiolase from Streptomyces coelicolor (428 aa); P76503|7449731|YFCY_ECOLI|D65007|B2342 probable 3-ketoacyl-CoA thiolase (acetyl-CoA acyltransferase) (beta-ketothiolase) from Escherichia coli strain K-12 (436 aa), FASTA scores: opt: 914, E(): 0, (38.2% identity in 434 aa overlap); P55084|ECHB_HUMAN mitochondrial trifunctonal enzyme (474 aa), FASTA scores: opt: 881, E(): 0, (37.7 identity in 451 aa overlap). Contains PS00099 Thiolases active site. Belongs to the thiolase family. O86361 No lipid metabolism O86361 O86361 GO:0008152,GO:0003988 2.3.1.16 O86361 NC_000962.3 Mycobrowser_v5 CDS 305825 306349 . - 0 Rv0254c cobU Involved in cobalamin biosynthesis. Probable bifunctional cobalamin biosynthesis protein CobU: cobinamide kinase + cobinamide phosphate guanylyltransferase Rv0254c, (MTV034.20), len: 174 aa. Probable cobU, cobalamin biosynthesis protein including a cobinamide kinase and cobinamide phosphate guanylyltransferase. Highly similar to many e.g. Q05599|COBU_SALTY cobinamide kinase / cobinamide phosphate guanylyltransferase from Salmonella typhimurium (181 aa), FASTA scores: opt: 308, E(): 1.1e-14, (38.7% identity in 181 aa overlap); P46886|COBU_ECOLI|B1993|Z3153|ECS2788 Bifunctional cobalamin biosynthesis protein cobU from Escherichia coli strains K12 and O157:H7 (181 aa); part of AL096872|SC5F7_10 from Streptomyces coelicolor (397 aa), FASTA scores: opt: 445, E(): 3.6e-23, (46.0% identity in 176 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). O53676 No intermediary metabolism and respiration O53676 O53676 GO:0043753,GO:0051188,GO:0000166,GO:0043752 2.-.-.- O53676 NC_000962.3 Mycobrowser_v5 CDS 1282306 1282893 . + 0 Rv1156 Rv1156 Function unknown Conserved protein Rv1156, (MTCI65.23), len: 195 aa. Conserved protein, highly similar to CAC32318.1|AL583944 conserved hypothetical protein from Streptomyces coelicolor (197 aa). O06554 No conserved hypotheticals O06554 O06554 GO:0003824,GO:0006284 O06554 NC_000962.3 Mycobrowser_v5 CDS 298863 300803 . - 0 Rv0248c Rv0248c Involved in interconversion of fumarate and succinate (aerobic respiration) [catalytic activity: succinate + acceptor = fumarate + reduced acceptor]. Probable succinate dehydrogenase [iron-sulfur subunit] (succinic dehydrogenase) Rv0248c, (MTV034.14c), len: 646 aa. Probable succinate dehydrogenase, flavoprotein subunit, highly similar to flavoprotein subunit of various succinate dehydrogenases e.g. M88696|RIRSDHA_1 flavoprotein from Rickettsia prowazekii (596 aa), FASTA scores: opt: 651, E(): 0, (34.6 % identity in 598 aa overlap). Also similar to truncated U00022_17 flavoprotein from Mycobacterium leprae (401 aa), FASTA scores: opt: 677, E(): 0, (39.0% identity in 423 aa overlap). Note that succinate dehydrogenase forms generally part of an enzyme complex containing four subunits: a flavoprotein (Rv0248c ?), an iron-sulfur (Rv0247c ?), and two hydrophobic anchor proteins (Rv0249c ?). O53670 No intermediary metabolism and respiration O53670 O53670 GO:0055114,GO:0000104,GO:0009055 1.3.99.1 O53670 NC_000962.3 Mycobrowser_v5 CDS 300834 301655 . - 0 Rv0249c Rv0249c Could be involved in interconversion of fumarate and succinate (aerobic respiration). This hydrophobic component may be required to anchor the catalytic components of the succinate dehydrogenase complex to the cytoplasmic membrane. Probable succinate dehydrogenase [membrane anchor subunit] (succinic dehydrogenase) Rv0249c, (MTV034.15c), len: 273 aa. Probable succinate dehydrogenase, membrane-anchor subunit for succinate dehydrogenase encoded by Rv0247c and Rv0248c. Highly similar to AC44315.1|AL596043 putative integral membrane protein from Streptomyces coelicolor (278 aa). Note that succinate dehydrogenase forms generally part of an enzyme complex containing four subunits: a flavoprotein (Rv0248c ?), an iron-sulfur (Rv0247c ?), and two hydrophobic anchor proteins (Rv0249c ?). O53671 No intermediary metabolism and respiration O53671 NC_000962.3 Mycobrowser_v5 CDS 301735 302028 . - 0 Rv0250c Rv0250c Function unknown. Possibly down-regulated by HSPR|Rv0353. Conserved protein Rv0250c, (MTV034.16c), len: 97 aa. Conserved protein, equivalent to MLCB1883.27c|T44883|3063888|CAA18576.1|AL022486 hypothetical protein from Mycobacterium leprae (98 aa), FASTA scores: opt: 478, E(): 4.4e-28, (72.6% identity in 95 aa overlap). Also similar to C-terminus of AC44316.1|AL596043|SCBAC31E11.05c hypothetical protein from Streptomyces coelicolor (146 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53672 No conserved hypotheticals O53672 NC_000962.3 Mycobrowser_v5 CDS 2472493 2473242 . + 0 Rv2208 cobS Involved in cobalamin biosynthesis Probable cobalamin 5'-phosphate synthase CobS Rv2208, (MTCY190.19), len: 249 aa. Probable cobS, cobalamin 5'-phosphate synthase; similarity to SW:COBS_ECOLI P36561 cobalamin (5'-phosphate) synthase (28.0% identity in 243 aa overlap) P9WP91 No intermediary metabolism and respiration Q10397 P9WP91 GO:0009236,GO:0015420,GO:0016021,GO:0005886,GO:0008818 2.-.-.- NC_000962.3 Mycobrowser_v5 CDS 302173 302652 . - 0 Rv0251c hsp Thought to be involved in the initiation step of translation at high temperature. Bound to 30S ribosomal subunit. Possibly a molecular chaperone. Seems to be regulated positively by SIGE|Rv1221 and negatively by HSPR|Rv0353. Heat shock protein Hsp (heat-stress-induced ribosome-binding protein A) Rv0251c, (MTV034.17c), len: 159 aa. Hsp (alternate gene name: hsp20, hrpA, acr2), heat-stress-induced ribosome-binding protein A (see citations below). Highly similar to AAD39038.1|AF072875_1|AF072875 putative HSP20 from Mycobacterium smegmatis (145 aa), FASTA scores: opt: 479, E(): 2.3e-24, (59.9% identity in 157 aa overlap); and similar to many bacterial and eukaryotic hsp proteins e.g. P12811|HS2C_CHLRE chloroplast heat shock 22KD protein from chlamydomonas reinhardtii (157 aa), FASTA scores: opt: 184, E(): 1.2e-05, (32.4% identity in 142 aa overlap). Also similar to PCC6803 Spore protein sp21 from Synechocystis sp. (146 aa), FASTA scores: opt: 213, E(): 1.2e-07, (30.3 identity in 145 aa overlap). Also similar to P30223|14KD_MYCTU 14 KDA antigen (16 KDA antigen) 19K major membrane protein (HSP 16.3) from Mycobacterium tuberculosis (144 aa). Belongs to the small heat shock protein (HSP20) family. O53673 No virulence, detoxification, adaptation O53673 O53673 GO:0006950 O53673 NC_000962.3 Mycobrowser_v5 CDS 2274569 2275408 . - 0 Rv2028c Rv2028c Unknown Universal stress protein family protein Rv2028c, (MTV018.15c), len: 279 aa. Universal stress protein family protein, highly similar to many, contains IPR006016 UspA domain. P9WFD9 No virulence, detoxification, adaptation O53474 P9WFD9 GO:0006950 P9WFD9 NC_000962.3 Mycobrowser_v5 CDS 1779314 1779724 . + 0 Rv1573 Rv1573 Unknown Probable PhiRv1 phage protein Rv1573, (MTCY336.31c), len: 136 aa. Probable phiRv1 phage protein (see citation below). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06617 No insertion seqs and phages O06617 NC_000962.3 Mycobrowser_v5 CDS 307877 309547 . - 0 Rv0256c PPE2 Function unknown PPE family protein PPE2 Rv0256c, (MTV034.22c), len: 556 aa. PPE2, Member of the M. tuberculosis PPE family, similar to many e.g. Rv0280, Rv0286, etc. Equivalent to Z98756|MLCB2492.30 from Mycobacterium leprae (572 aa), FASTA scores: opt: 1837, E(): 0, (62.9% identity in 461 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WI47 No PE/PPE Q79FZ4 P9WI47 P9WI47 NC_000962.3 Mycobrowser_v5 CDS 309699 310073 . + 0 Rv0257 Rv0257 Function unknown Conserved hypothetical protein Rv0257, len: 124 aa. Hypothetical protein, orthologue of ML1828A conserved hypothetical protein from Mycobacterium leprae. Replaced Rv0257c (older annotation). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). L7N694 No conserved hypotheticals L7N694 NC_000962.3 Mycobrowser_v5 CDS 310294 310749 . - 0 Rv0258c Rv0258c Function unknown Conserved hypothetical protein Rv0258c, (MTCY06A4.02c), len: 151 aa (alternative start possible). Conserved hypothetical protein, showing some similarity to Rv1685c|MTCI125_6 from Mycobacterium tuberculosis (207 aa), FASTA scores: E(): 9.3e-07, (32.1% identity in 140 aa overlap). Also some similarity with AL049819|SCE7_13|T36295 probable transcription regulator from Streptomyces coelicolor (204 aa), FASTA scores: opt: 158, E(): 0.00052, (27.0% identity in 111 aa overlap). P95215 No conserved hypotheticals P95215 P95215 NC_000962.3 Mycobrowser_v5 CDS 311514 312659 . - 0 Rv0260c Rv0260c Could be involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0260c, (MTCY0A4.04c), len: 381 aa. Possible two-component response regulator, highly similar to CAB72204.1|AL138851 putative transcriptional regulator from Streptomyces coelicolor (395 aa); and similar to O34394|D69851|YJJA conserved hypothetical protein from Bacillus subtilis (270 aa), FASTA scores: opt: 312, E(): 7.4e-14, (25.8% identity in 267 aa overlap). Also some similarity to regulatory proteins at C-terminal region e.g. CUTR_STRLI|Q03756 transcriptional regulatory protein (217 aa), FASTA scores: opt: 138, E(): 0.02, (30.6% identity in 111 aa overlap). P95217 No regulatory proteins P95217 P95217 GO:0005622,GO:0006355,GO:0033014,GO:0000156,GO:0000160,GO:0004852,GO:0003677 P95217 NC_000962.3 Mycobrowser_v5 CDS 358945 359748 . - 0 Rv0295c Rv0295c Function unknown Conserved protein Rv0295c, (MTV035.23c), len: 267 aa. Conserved protein, showing weak similarity with CAC46877.1|AL591790 conserved hypothetical protein from Sinorhizobium meliloti (213 aa); and NP_104818.1|14023999|BAB50604.1|AP00300 Protein with weak similarity to NodH from Mesorhizobium loti (257 aa). Predicted to be an outer membrane protein (See Song et al., 2008). O53699 No conserved hypotheticals O53699 O53699 NC_000962.3 Mycobrowser_v5 CDS 455637 455858 . + 0 Rv0378 Rv0378 Function unknown Conserved hypothetical glycine rich protein Rv0378, (MTV036.13), len: 73 aa. Conserved hypothetical gly-rich protein, showing some similarity to Mycobacterium tuberculosis PE_PGRS family; also similar to MTCY06H11_16|Z85982 hypothetical glycine-rich 88.5 KD protein (1011 aa), FASTA scores: opt: 237, E(): 0.0032, (58.7% identity in 63 aa overlap); MTV043_25. O53713 No conserved hypotheticals O53713 NC_000962.3 Mycobrowser_v5 CDS 314864 315766 . - 0 Rv0263c Rv0263c Function unknown Conserved hypothetical protein Rv0263c, (MTCY06A4.07c), len: 300 aa. Conserved hypothetical protein, equivalent to NP_302634.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (305 aa). Also similar to others e.g. AL121596|SC51A_21 hypothetical protein from Streptomyces coelicolor (285 aa), FASTA scores: opt: 714, E(): 0, (45.3% identity in 289 aa overlap); NP_233164.1|NC_002506 conserved hypothetical protein from Vibrio cholerae (309 aa); NP_406216.1|NC_003143 conserved hypothetical protein from Yersinia pestis (316 aa); YH30_HAEIN|P44298|hi1730 hypothetical protein from Haemophilus influenzae (309 aa), FASTA scores: opt: 430, E(): 3e-20, (29.6% identity in 284 aa overlap); etc. Also similar to carboxylases eg NP_415240.1|NC_000913|P75745|YBGK_ECOLI putative carboxylase from Escherichia coli strain K12 (310 aa), FASTA score: (34.6% identity in 286 aa overlap); NP_459698.1|NC_003197 putative carboxylase from Salmonella typhimurium (310 aa); and to middle part of NP_420636.1|NC_002696 urea amidolyase-related protein from Caulobacter crescentus (1207 aa). P95220 No conserved hypotheticals P95220 P95220 P95220 NC_000962.3 Mycobrowser_v5 CDS 315783 316415 . - 0 Rv0264c Rv0264c Function unknown Conserved hypothetical protein Rv0264c, (MTCY06A4.08c), len: 210 aa. Conserved hypothetical protein, equivalent to CAC32080.1|AL583926 conserved hypothetical protein from Mycobacterium leprae (222 aa). Also similar to others hypothetical proteins e.g. AL121596|SC51A_20 from Streptomyces coelicolor (252 aa), FASTA scores: opt: 420, E(): 2.7e-20, (41.7% identity in 204 aa overlap); P75744|YBGJ_ECOLI hypothetical 23.9 KD protein from Escherichia coli (218 aa), FASTA scores: E(): 2.1e-14, (35.7% identity in 182 aa overlap); YH31_HAEIN|P44299|hi173 hypothetical protein from Haemophilus influenzae (213 aa), FASTA scores: opt: 252, E(): 8.3e-10, (31.1% identity in 183 aa overlap). P95221 No conserved hypotheticals P95221 P95221 P95221 NC_000962.3 Mycobrowser_v5 CDS 322764 323273 . - 0 Rv0268c Rv0268c Unknown Hypothetical protein Rv0268c, (MTCY06A4.12c), len: 169 aa. Hypothetical unknown protein. P95225 No conserved hypotheticals P95225 P95225 NC_000962.3 Mycobrowser_v5 CDS 351848 352138 . + 0 Rv0288 esxH Function unknown. May be involved in virulence. Low molecular weight protein antigen 7 EsxH (10 kDa antigen) (CFP-7) (protein TB10.4) Rv0288, (MT0301, MTV035.16), len: 96 aa. EsxH, low molecular weight protein antigen 7 (10 kDa antigen) (CFP-7) (Protein TB10.4) (see citations below), ala-rich protein; member of mycobacterial protein family containing ESAT-6, very similar to MTV012_33 from Mycobacterium tuberculosis (96 aa), FASTA scores: opt: 566, E(): 0, (84.4% identity in 96 aa overlap). Alternative start codon possible position 351878 (see Rosenkrands et al., 2000). Belongs to the ESAT6 family (see Skjot et al., 2000; 2002; Gey Van Pittius et al., 2001). Note that previously known as cfp7 (alternate gene name: TB10.4). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted possible vaccine candidate (See Zvi et al., 2008). P9WNK3 No cell wall and cell processes 2KG7 P9WNK3 P9WNK3 NC_000962.3 Mycobrowser_v5 CDS 1961291 1961788 . - 0 Rv1735c Rv1735c Function unknown Hypothetical membrane protein Rv1735c, (MTCY04C12.20c), len: 165 aa. Hypothetical membrane protein, similar to part of O58614|PH0884|AP000004 Hypothetical malic acid transport protein from Pyrococcus horikoshii (330 aa), FASTA scores: opt: 167, E(): 0.0003, (29.2% identity in 120 aa overlap). P9WLS5 No cell wall and cell processes P71993 P9WLS5 GO:0005886,GO:0055085,GO:0016021 P9WLS5 NC_000962.3 Mycobrowser_v5 CDS 317525 321154 . - 0 Rv0266c oplA Catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate [catalytic activity: ATP + 5-oxo-L-proline + 2 H2O = ADP + phosphate + L-glutamate]. Probable 5-oxoprolinase OplA (5-oxo-L-prolinase) (pyroglutamase) (5-OPASE) Rv0266c, (MTCY06A4.10c), len: 1209 aa. Probable oplA, 5-oxoprolinase, highly similar to others or to hypothetical proteins e.g. AAK24340.1|AE005906 hydantoinase/oxoprolinase from Caulobacter crescentus (1196 aa); NP_103129.1|14022305|BAB48915.1|AP002997 5-oxoprolinase from Mesorhizobium loti (1210 aa); CAC48426.1|AL603642 conserved hypothetical protein from Sinorhizobium meliloti (1205 aa); S77037|slr0697|1006579|BAA10729.1|D6400 hypothetical protein from Synechocystis sp. strain PCC 6803 (1252 aa), FASTA scores: opt: 2016, E(): 0, (51.4% identity in 1247 aa overlap); P97608|OPLA_RAT|T42756|11278797 5-oxoprolinase (5-oxo-L-prolinase) (pyroglutamase) (5-OPASE) from Rattus norvegicus (1288 aa); etc. Belongs to the oxoprolinase family. P95223 No intermediary metabolism and respiration P95223 P95223 GO:0017168 3.5.2.9 P95223 NC_000962.3 Mycobrowser_v5 CDS 323338 324531 . - 0 Rv0269c Rv0269c Function unknown Conserved hypothetical protein Rv0269c, (MTCY06A4.13c), len: 397 aa. Conserved hypothetical protein, highly similar to AL079355|SC4C6_19 hypothetical protein from Streptomyces coelicolor (341 aa), FASTA scores: opt: 1019, E(): 0, (46.5% identity in 344 aa overlap), and similar to other proteins e.g. CAC49016.1|AL603644 putative ATP-dependent DNA ligase protein from Sinorhizobium meliloti (636 aa); O34398 YKOU protein from Bacillus subtilis (611 aa), FASTA score: (27.2% identity in 283 aa overlap). Also similar to proteins from Mycobacterium tuberculosis e.g. Rv3062, Rv3731 (both DNA ligases), and Rv0938, Rv3730c. P95226 No conserved hypotheticals P95226 P95226 GO:0006269,GO:0003896 P95226 NC_000962.3 Mycobrowser_v5 CDS 469591 470013 . + 0 Rv0390 Rv0390 Function unknown Conserved protein Rv0390, (MTCY04D9.02), len: 140 aa. Conserved protein, equivalent to AL023514|MLCB4_11|CAA18942.1|AL023514 hypothetical protein from Mycobacterium leprae (147 aa), FASTA scores: opt: 778, E(): 0, (79.0% identity in 138 aa overlap). Also similar to hypothetical proteins from several Rickettsia species. P95198 No conserved hypotheticals 2FSX P95198 P95198 P95198 NC_000962.3 Mycobrowser_v5 CDS 324567 326249 . + 0 Rv0270 fadD2 Function unknown, but involved in lipid degradation. Probable fatty-acid-CoA ligase FadD2 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv0270, (MTCY06A4.14), len: 560 aa. Probable fadD2, fatty-acid-CoA synthetase, similar to many e.g. LCFA_ECOLI|P29212 long-chain-fatty-acid--CoA ligase from Escherichia coli (561 aa), FASTA scores: opt: 544, E(): 2.9e-26, (27.7% identity in 535 aa overlap). Also similar to others from Mycobacterium tuberculosis e.g. MTCY493_2, MTCY8D5_9, MTCY6G11_8, etc. Contains PS00455 Putative AMP-binding domain signature. P95227 No lipid metabolism P95227 P95227 GO:0008152,GO:0016874 6.2.1.- P95227 NC_000962.3 Mycobrowser_v5 CDS 326266 328461 . - 0 Rv0271c fadE6 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE6 Rv0271c, (MTCY06A4.15c), len: 731 aa. Probable fadE6, acyl-CoA dehydrogenase, with C-terminal half similar to many e.g. ACDS_HUMAN|P16219 acyl-CoA dehydrogenase (short-chain) from Homo sapiens (412 aa), FASTA scores: opt: 339, E(): 1.3e-13, (28.1% identity in 288 aa overlap). P95228 No lipid metabolism P95228 P95228 GO:0003995,GO:0055114,GO:0050660 1.3.99.- P95228 NC_000962.3 Mycobrowser_v5 CDS 379172 380401 . + 0 Rv0311 Rv0311 Unknown Unknown protein Rv0311, (MTCY63.16), len: 409 aa. Unknown protein. Contains PS00881 Protein splicing signature. O07238 No conserved hypotheticals O07238 O07238 NC_000962.3 Mycobrowser_v5 CDS 392039 392263 . + 0 Rv0325 Rv0325 Unknown Hypothetical protein Rv0325, (MTCY63.30), len: 74 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07250 No unknown O07250 NC_000962.3 Mycobrowser_v5 CDS 2123684 2124370 . + 0 Rv1874 Rv1874 Unknown Unknown protein Rv1874, (MTCY180.44c), len: 228 aa. Unknown protein. O07755 No conserved hypotheticals O07755 NC_000962.3 Mycobrowser_v5 CDS 330933 331658 . - 0 Rv0275c Rv0275c Could be involved in transcriptional mechanism. Possible transcriptional regulatory protein (possibly TetR-family) Rv0275c, (MTV035.03c), len: 241 aa. Possible transcriptional regulator, TetR family, similar to others e.g. Q9RJE7|SCF81.04c putative TetR-family transcriptional regulator from Streptomyces coelicolor (219 aa); Q9FBI8|SCP8.33c putative TetR-family transcriptional regulator from Streptomyces coelicolor (213 aa); Q9I2Q9|PA1836 probable transcriptional regulator from Pseudomonas aeruginosa (193 aa); etc. Also shows some similarity with Rv0825c from Mycobacterium tuberculosis (213 aa), FASTA scores: opt: 230, E(): 2.7e-07, (32.6% identity in 190 aa overlap). Seems to belong to the TetR/AcrR family of transcriptional regulators (M. tuberculosis regulatory protein family with many TetR orthologues). L7N6A2 No regulatory proteins Q7DA42 L7N6A2 GO:0006350,GO:0003700,GO:0006355 L7N6A2 NC_000962.3 Mycobrowser_v5 CDS 331748 332668 . + 0 Rv0276 Rv0276 Function unknown Conserved hypothetical protein Rv0276, (MTV035.04), len: 306 aa. Conserved hypothetical protein, similar to Rv2237|Z70692|MTCY427.18 from Mycobacterium tuberculosis (296 aa), FASTA scores: opt: 874, E(): 0, (49.6% identity in 282 aa overlap). O53682 No conserved hypotheticals O53682 O53682 O53682 NC_000962.3 Mycobrowser_v5 CDS 332708 333136 . - 0 Rv0277c vapC25 Unknown Possible toxin VapC25. Contains PIN domain. Rv0277c, (MTV035.05c), len: 142 aa. Possible vapC25, toxin, part of toxin-antitoxin (TA) operon with Rv0277A, contains PIN domain, see Arcus et al. 2005. Highly similar to others e.g. Rv0749|H70824|2911023|CAA17516.1|AL021958 conserved hypothetical protein from Mycobacterium tuberculosis (142 aa); and Rv2530c, etc. P9WF85 No virulence, detoxification, adaptation O53683 P9WF85 P9WF85 NC_000962.3 Mycobrowser_v5 CDS 336560 339073 . - 0 Rv0279c PE_PGRS4 Function unknown PE-PGRS family protein PE_PGRS4 Rv0279c, (MTV035.07c), len: 837 aa. PE_PGRS4, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), similar to many e.g. Z95890|MTCY28_25|Rv0278c from Mycobacterium tuberculosis (914 aa), FASTA scores: opt: 2677, E(): 0, (64.5% identity in 926 aa overlap). L0T4W6 No PE/PPE Q79FY9 L0T4W6 L0T4W6 NC_000962.3 Mycobrowser_v5 CDS 345635 349627 . + 0 Rv0284 eccC3 Unknown ESX conserved component EccC3. ESX-3 type VII secretion system protein. Possible membrane protein. Rv0284, (MTV035.12), len: 1330 aa. eccC3, esx conserved component, ESX-3 type VII secretion system protein, possible membrane protein, similar to products of two adjacent Mycobacterium leprae genes, MLCB628.16c (744 aa) and MLCB628.15c (597 aa); and throughout its length to several large Mycobacterium tuberculosis proteins: Rv3447c, Rv3870, Rv1784, etc. Y14967|MLCB628_ 15 (744 aa), FASTA scores: opt: 942, E(): 0, (33.8% identity in 730 aa overlap); Y14967|MLCB628_14 (597 aa), FASTA scores: opt: 613, E(): 3.1e-30, (31.7% identity in 615 aa overlap); Z94121|MTY15F10_17 (1396 aa), FASTA scores: opt: 652, E(): 2.2e-32, (35.4% identity in 1321 aa overlap); Z95389|MTCY77_19 (1236 aa), FASTA scores: opt 652, E(): 2.2e-32, (35.4% identity in 1321 aa overlap). Contains three PS00017 ATP/GTP-binding site motif A (P-loop). P9WNA9 No cell wall and cell processes O53689 P9WNA9 GO:0003677,GO:0007049,GO:0051301,GO:0007059,GO:0016021,GO:0017111,GO:0005886,GO:0005524 P9WNA9 NC_000962.3 Mycobrowser_v5 CDS 344022 345638 . + 0 Rv0283 eccB3 Unknown ESX conserved component EccB3. ESX-3 type VII secretion system protein. Possible membrane protein. Rv0283, (MTV035.11), len: 538 aa. eccB3, esx conserved component, ESX-3 type VII secretion system protein, possible membrane protein, similar to several hypothetical mycobacterial proteins e.g. Z94121|MTY15F10_16|Rv3895c from Mycobacterium tuberculosis (495 aa), FASTA scores: opt: 698, E(): 0, (37.6% identity in 492 aa overlap); Rv1782; Rv3450c; Rv3869; and Y14967|MLCB628_16|MLCB628.17c from Mycobacterium leprae (481 aa), FASTA scores: opt: 672, E(): 1.5e-31, (37.2% identity in 506 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WNR3 No cell wall and cell processes O53688 P9WNR3 GO:0005886,GO:0016021 P9WNR3 NC_000962.3 Mycobrowser_v5 CDS 353083 354501 . + 0 Rv0290 eccD3 Unknown ESX conserved component EccD3. ESX-3 type VII secretion system protein. Probable transmembrane protein. Rv0290, (MTV035.18), len: 472 aa. EccD3, esx conserved component, ESX-3 type VII secretion system protein, probable transmembrane protein, similar to several others in mycobacteria e.g. Z95389|MTCY77_20|Rv3887c from Mycobacterium tuberculosis (467 aa), FASTA scores: opt: 429, E(): 5.1e-19, (28. 6% identity in 479 aa overlap); Rv3877; Rv1795; Rv3448; and Y14967|MLCB628_9|MLCB628.10c from Mycobacterium leprae (480 aa), FASTA scores: opt: 269, E(): 3.1e-09, (26.0% identity in 503 aa overlap). P9WNQ3 No cell wall and cell processes O86362 P9WNQ3 GO:0005886,GO:0016021 P9WNQ3 NC_000962.3 Mycobrowser_v5 CDS 384535 385149 . + 0 Rv0316 Rv0316 Could be involved in the catabolism of catechol to succinate- and acetyl-CoA in the beta-ketoadipate pathway (at the third step) [catalytic activity: 2,5-dihydro-5-oxofuran-2-acetate = 3,4-dihydro-5-oxofuran-2-acetate]. Possible muconolactone isomerase Rv0316, (MTCY63.21), len: 204 aa. Possible muconolactone isomerase, showing weak similarity with some muconolactone isomerases e.g. O33947|CTC1_ACILW muconolactone delta-isomerase 1 (MIASE 1)(96 aa), FASTA scores: opt: 179, E(): 3.9e-05, (32.6% identity in 92 aa overlap). O07243 No intermediary metabolism and respiration O07243 O07243 GO:0016853,GO:0006725 5.3.3.- O07243 NC_000962.3 Mycobrowser_v5 CDS 398254 398628 . + 0 Rv0333 Rv0333 Unknown Unknown protein Rv0333, (MTCY63.38), len: 124 aa. Unknown protein. O33273 No conserved hypotheticals O33273 O33273 NC_000962.3 Mycobrowser_v5 CDS 3315236 3316456 . + 0 Rv2963 Rv2963 Unknown Probable integral membrane protein Rv2963, (MTCY349.24c), len: 406 aa. Probable integral membrane protein. I6YET7 No cell wall and cell processes P95129 I6YET7 NC_000962.3 Mycobrowser_v5 CDS 355880 356875 . + 0 Rv0292 eccE3 Unknown ESX conserved component EccE3. ESX-3 type VII secretion system protein. Probable transmembrane protein. Rv0292, (MTV035.20), len: 331 aa. EccE3, esx conserved component, ESX-3 type VII secretion system protein, probable transmembrane protein (has two hydrophobic segments at N-terminal end), equivalent to CAC32058.1|AL583926 conserved membrane protein from Mycobacterium leprae (339 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WJE5 No cell wall and cell processes O53696 P9WJE5 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 356862 358064 . - 0 Rv0293c Rv0293c Function unknown Conserved protein Rv0293c, (MTV035.21c), len: 400 aa. Conserved protein, similar in C-terminal part to Rv2627c|B70573|MTCY01A10.05|CAB08637.1|Z95387 conserved hypothetical protein from Mycobacterium tuberculosis (413 aa), FASTA scores: opt: 394, E(): 2.1e-17, (31.1% identity in 299 aa overlap). O53697 No conserved hypotheticals O53697 O53697 O53697 NC_000962.3 Mycobrowser_v5 CDS 358171 358956 . + 0 Rv0294 tam Possibly catalyzes the S-adenosylmethionine monomethyl esterification of trans-aconitate at high affinity and of cis-aconitate, isocitrate, and citrate at lower velocities and affinities. Probable trans-aconitate methyltransferase Tam Rv0294, (MTV035.22), len: 261 aa. Probable tam, trans-aconitate methyltransferase, similar to others e.g. P76145|TAM_ECOLI|7465793|B64906|B1519 trans-aconitate methyltransferase from Escherichia coli strain K12 (252 aa), FASTA scores: opt: 649, E(): 0, (39.3 identity in 252 aa overlap). Belongs to the methyltransferase superfamily. P9WGA3 No intermediary metabolism and respiration P66885 P9WGA3 GO:0008152,GO:0030798,GO:0005737 2.1.1.144 P9WGA3 NC_000962.3 Mycobrowser_v5 CDS 442395 443078 . + 0 Rv0364 Rv0364 Unknown Possible conserved transmembrane protein Rv0364, (MTCY13E10.26), len: 227 aa. Possible conserved transmembrane protein, equivalent to O69601|Y364_MYCLE|ML0287|CAA18951.1|AL023514|AL023514|MLCB4_19 hypothetical 24.3 KDA protein from Mycobacterium leprae (222 aa), FASTA scores: opt: 1027, E(): 0, (66.1% identity in 227 aa overlap). Shows strong similarity to DEDA_ECOLI|P09548 DedA protein protein from Escherichia coli FASTA scores: E(): 1.3e-28, (39.5% identity in 195 aa overlap). Similar also to Mycobacterium tuberculosis DedA protein Rv2637|MTCY441.0. P9WP09 No cell wall and cell processes O06314 P9WP09 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 600098 600391 . + 0 Rv0508 Rv0508 Function unknown Conserved hypothetical protein Rv0508, (MTCY20G9.35), len: 97 aa. Conserved hypothetical protein, showing similarity with T36269|5123666|CAB45355.1|AL079345 probable redoxin from Streptomyces coelicolor (101 aa), FASTA scores: opt: 160, E(): 3.4e-05, (33.3% identity in 75 aa overlap); and E81943|NMA0966 probable thioredoxin from Neisseria meningitidis group a strain Z2491 (77 aa). P9WKS9 No conserved hypotheticals P64727 P9WKS9 NC_000962.3 Mycobrowser_v5 CDS 365234 366142 . + 0 Rv0303 Rv0303 Function unknown; probably involved in cellular metabolism. Probable dehydrogenase/reductase Rv0303, (MTCY63.08), len: 302 aa. Possible dehydrogenase/reductase, similar to various NADPH dehydrogenases and other NADPH oxidoreductases e.g. O48741|PORC_ARATH|7488284|T00897 protochlorophyllide reductase C chloroplast precursor (NADPH-protochlorophyllide oxidoreductase C) from Arabidopsis thaliana (401 aa); Q42850 NADPH dehydrogenase (395 aa), FASTA scores: opt: 347, E(): 3.8e-16, (35.4% identity in 319 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07230 No intermediary metabolism and respiration O07230 O07230 GO:0055114,GO:0016491 1.-.-.- O07230 NC_000962.3 Mycobrowser_v5 CDS 363252 363479 . + 0 Rv0298 Rv0298 Unknown Hypothetical protein Rv0298, (MTCY63.03), len: 75 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WJ09 No conserved hypotheticals O07225 P9WJ09 GO:0006355,GO:0003677 NC_000962.3 Mycobrowser_v5 CDS 363476 363778 . + 0 Rv0299 Rv0299 Unknown Hypothetical protein Rv0299, (MTCY63.04), len: 100 aa. Hypothetical unknown protein. Equivalent to AAK44536.1 from Mycobacterium tuberculosis strain CDC1551 (49 aa) but longer 51 aa. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07226 No conserved hypotheticals O07226 NC_000962.3 Mycobrowser_v5 CDS 363826 364047 . + 0 Rv0300 vapB2 Unknown Possible antitoxin VapB2 Rv0300, (MTCY63.05), len: 73 aa. Possible vapB2, antitoxin, part of toxin-antitoxin (TA) operon with Rv0301 (See Arcus et al., 2005; Pandey and Gerdes, 2005). Weak similarity with others e.g. Rv3697c from Mycobacterium tuberculosis (145 aa), Rv1721c|MTCY04C12.06c|Z81360|MTCY4C12_4 conserved hypothetical protein from Mycobacterium tuberculosis (75 aa), FASTA scores: opt: 84, E(): 8.3, (39.5% identity in 38 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07227 No virulence, detoxification, adaptation 3H87 O07227 O07227 GO:0006355,GO:0003677 O07227 NC_000962.3 Mycobrowser_v5 CDS 392273 392728 . + 0 Rv0326 Rv0326 Unknown Hypothetical protein Rv0326, (MTCY63.31), len: 151 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07251 No unknown O07251 O07251 GO:0008168,GO:0008152 O07251 NC_000962.3 Mycobrowser_v5 CDS 397442 398227 . + 0 Rv0332 Rv0332 Function unknown Conserved protein Rv0332, (MTCY63.37), len: 261 aa. Conserved protein, similar to several conserved hypothetical proteins from Streptomyces coelicolor e.g. SC6A9.18c|AL031035|SC6A9_18|T35449 hypothetical protein (266 aa), FASTA scores: opt: 508, E(): 5.7e-27, (36.7% identity in 251 aa overlap). O07256 No conserved hypotheticals O07256 O07256 O07256 NC_000962.3 Mycobrowser_v5 CDS 417304 418290 . + 0 Rv0347 Rv0347 Unknown Probable conserved membrane protein Rv0347, (MTCY13E10.07), len: 328 aa (alternative start possible). Probable conserved membrane protein, similar to Rv0831c|AL022004|MTV043_23 from Mycobacterium tuberculosis (271 aa), FASTA scores: E(): 9.6e-21, (33.1% identity in 266 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06298 No cell wall and cell processes O06298 O06298 NC_000962.3 Mycobrowser_v5 CDS 731712 731879 . + 0 Rv0634B rpmG2 Involved in translation mechanism 50S ribosomal protein L33 RpmG2 Rv0634B, len: 55 aa. rpmG2, 50S ribosomal protein L33. Note that Mycobacterium tuberculosis has a second rpmG gene: P96925|R33H_MYCTU|Rv2057c|MTCY63A.03|rpmG1 putative 50S ribosomal protein L33 (55 aa), FASTA scores: opt: 391, E(): 2.9e-25, (100.0% identity in 55 aa overlap). Belongs to the L33P family of ribosomal proteins. P9WH95 No information pathways P0A5W2 P9WH95 GO:0003735,GO:0006412,GO:0005840 P9WH95 NC_000962.3 Mycobrowser_v5 CDS 377113 377829 . + 0 Rv0308 Rv0308 Unknown Probable conserved integral membrane protein Rv0308, (MTCY63.13), len: 238 aa. Probable conserved integral membrane protein, with C-terminus highly similar to C-terminus of other integral membrane proteins or phosphatases e.g. AAK25788.1|AF336822_1|13430250|AAK25789.1|AF336823_1 putative phosphatase from Streptococcus pyogenes (201 aa); Q06074 hypothetical 24.9 kDa protein (216 aa), FASTA scores: opt: 209, E(): 2e-07, (27.9% identity in 140 aa overlap). Could be a phosphatase. O07235 No cell wall and cell processes O07235 O07235 GO:0016020,GO:0003824 O07235 NC_000962.3 Mycobrowser_v5 CDS 377931 378587 . + 0 Rv0309 Rv0309 Unknown Possible conserved exported protein Rv0309, (MTCY63.14), len: 218 aa. Possible conserved exported protein (has putative N-terminal signal sequence), equivalent to AC32053.1|AL583926 putative secreted protein from Mycobacterium leprae (218 aa). Also similar to others e.g. AB76092.1|AL157956 putative secreted protein from Streptomyces coelicolor (238 aa). Predicted to be an outer membrane protein (See Song et al., 2008). O07236 No cell wall and cell processes O07236 O07236 O07236 NC_000962.3 Mycobrowser_v5 CDS 378657 379148 . - 0 Rv0310c Rv0310c Function unknown Conserved protein Rv0310c, (MTCY63.15c), len: 163 aa. Conserved protein, similar to some bile acid dehydratases e.g. P19412|BAIE_EUBSP|98749|D37844|1381566|AAC45413.1|U57489 bile acid-inducible operon protein E from Eubacterium sp (166 aa), FASTA scores: opt: 302, E(): 1e-11, (38.8% identity in 134 aa overlap); AAF22847.1|AF210152_4 bile acid 7a-dehydratase from Clostridium sp. (168 aa). O07237 No conserved hypotheticals O07237 O07237 NC_000962.3 Mycobrowser_v5 CDS 380556 382418 . + 0 Rv0312 Rv0312 Function unknown Conserved hypothetical proline and threonine rich protein Rv0312, (MTCY63.17), len: 620 aa. Conserved hypothetical protein with highly Pro-, Thr-rich C-terminus. Similar to Pro-,Thr-rich region in Rv2264c|AL021925|MTV022_14 from Mycobacterium tuberculosis (592 aa), FASTA scores: opt: 1075, E(): 0, (38.9% identity in 627 aa overlap). Also some similarity with Rv0350|dnaK from Mycobacterium tuberculosis. Possibly membrane protein; has hydrophobic stetch in its middle part. O07239 No conserved hypotheticals O07239 O07239 GO:0005524 O07239 NC_000962.3 Mycobrowser_v5 CDS 383602 384486 . + 0 Rv0315 Rv0315 Possibly hydrolyzes specific sugar (hydrolyzation of glycosidic bond) and could be involved in exopolysaccharide biosynthesis/degradation. Could also have a LYTIC activity against cell WALLS. Possible beta-1,3-glucanase precursor Rv0315, (MTCY63.20), len: 294 aa. Possible beta-1,3-glucanase precursor (has hydrophobic stretch in its N-terminal part), similar to others e.g. Q51333|AAC44371.1 beta-1,3-glucanase II a from Oerskovia xanthineolytica (306 aa), FASTA scores: opt: 76, E(): 3e-14, (34.1% identity in 302 aa overlap); and AAC38290.1|AF052745 beta-1,3-glucanase II from Oerskovia xanthineolytica (435 aa). Contains glycosyl hydrolases family 16 active site signature (PS01034). O07242 No intermediary metabolism and respiration O07242 O07242 GO:0004553,GO:0005975 3.2.1.- O07242 NC_000962.3 Mycobrowser_v5 CDS 589183 590058 . + 0 Rv0499 Rv0499 Function unknown Conserved hypothetical protein Rv0499, (MTCY20G9.25), len: 291 aa. Conserved hypothetical protein, showing some similarity to AL031184|SC2A11_16|T34762 hypothetical protein from Streptomyces coelicolor (340 aa), FASTA scores: opt: 240, E(): 1.8e-07, (28.9% identity in 270 aa overlap). P9WKT9 No conserved hypotheticals P9WKT9 GO:0004143,GO:0007205 P9WKT9 NC_000962.3 Mycobrowser_v5 CDS 386305 387099 . - 0 Rv0318c Rv0318c Unknown Probable conserved integral membrane protein Rv0318c, (MTCY63.23c), len: 264 aa. Probable conserved integral membrane protein, with some similarity to C-terminus of GUFA_MYXXA|Q06916 (254 aa), FASTA scores: opt: 157, E (): 0.0032, (28.3% identity in 198 aa overlap). Also similar to O26573 conserved protein from Methanobacterium thermoauto (259 aa), FASTA scores: opt: 173, E(): 5.2e-05, (32.7% identity in 214 aa overlap). Q6MX47 No cell wall and cell processes Q6MX47 Q6MX47 GO:0046873,GO:0030001,GO:0055085,GO:0016020 Q6MX47 NC_000962.3 Mycobrowser_v5 CDS 387148 387816 . + 0 Rv0319 pcp Removes 5-oxoproline from various penultimate amino acid residues except L-proline [catalytic activity: 5-oxoprolyl-peptide + H2O = 5-oxoproline + peptide]. Probable pyrrolidone-carboxylate peptidase Pcp (5-oxoprolyl-peptidase) (pyroglutamyl-peptidase I) (PGP-I) (pyrase) Rv0319, (MTCY63.24), len: 222 aa. Probable pcp, pyrrolidone-carboxylate peptidase, highly similar to others e.g. PCP_PSEFL|P42673 pyrrolidone-carboxylate peptidase from Pseudomonas fluorescens (213 aa), FASTA scores: opt: 478, E(): 7.5e-25, (40.2% identity in 219 aa overlap). Belongs to peptidase family C15 (thiol protease). P9WIJ5 No intermediary metabolism and respiration P0A5R4 P9WIJ5 GO:0005737,GO:0006508,GO:0016920,GO:0008234 3.4.19.3 P9WIJ5 NC_000962.3 Mycobrowser_v5 CDS 388582 389154 . + 0 Rv0321 dcd Involved in interconversion of dCTP and dUTP [catalytic activity: dCTP + H2O = dUTP + NH3]. Probable deoxycytidine triphosphate deaminase Dcd (dCTP deaminase) Rv0321, (MTCY63.26), len: 190 aa. Probable dcd (alternate gene names: dus or paxA), deoxycytidine triphosphate deaminase, equivalent to CAC32024.1|AL583925 probable deoxycytidine triphosphate deaminase from Mycobacterium leprae (190 aa). Also highly similar to others e.g. Q9X8W0|DCD_STRCO|7480599|T36613|SCH35.46 deoxycytidine triphosphate deaminase from Streptomyces coelicolor (191 aa); DCD_ECOLI|P28248|DUS|PAXA|B2065 deoxycytidine triphosphate deaminase from Escherichia coli strain K12 (193 aa), FASTA scores: opt: 408, E(): 2.7e-21, (43.1% identity in 188 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the dCTP deaminase family. The transcription of this CDS seems to be activated specifically in host granulomas (see citation below). P9WP17 No intermediary metabolism and respiration 2QLP,2QXX O07247 P9WP17 GO:0006229,GO:0005515,GO:0009220,GO:0008829 3.5.4.13 P9WP17 NC_000962.3 Mycobrowser_v5 CDS 389260 390591 . + 0 Rv0322 udgA Possibly involved in polysaccharide biosynthesis [catalytic activity: UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH]. Probable UDP-glucose 6-dehydrogenase UdgA (UDP-GLC dehydrogenase) (UDP-GLCDH) (UDPGDH) Rv0322, (MTCY63.27), len: 443 aa. Probable udg (alternate gene name: rkpK), UDP-glucose 6-dehydrogenase , highly similar to others e.g. CAC44517.1|AL596138 putative UDP-glucose 6-dehydrogenase from Streptomyces coelicolor (447 aa); Q56812 UDP-glucose dehydrogenase from Xanthomonas campestris (445 aa), FASTA scores: opt: 713, E(): 0, (41.9% identity in 351 aa overlap); etc. Also similar to several GDP-mannose 6-dehydrogenase. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the UDP-glucose/GDP-mannose dehydrogenases family. O07248 No intermediary metabolism and respiration O07248 O07248 GO:0003979,GO:0055114,GO:0051287 1.1.1.22 O07248 NC_000962.3 Mycobrowser_v5 CDS 390580 391251 . - 0 Rv0323c Rv0323c Function unknown Conserved hypothetical protein Rv0323c, (MTCY63.28c), len: 223 aa. Conserved hypothetical protein, similar to others e.g. YPJG_BACSU|P42981 hypothetical 24.8 kDa protein from Bacillus subtilis (224 aa), FASTA scores: opt: 182, E(): 1.3e-05, (27.5% identity in 211 aa overlap). Also some similarity to MLU15183_8 from Mycobacterium tuberculosis FASTA score: (32.0% identity in 147 aa overlap). Alternative nucleotide at position 390828 (T->C; S142G) has been observed. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L0T643 No conserved hypotheticals O07249 L0T643 L0T643 NC_000962.3 Mycobrowser_v5 CDS 421709 422416 . + 0 Rv0351 grpE Stimulates, jointly with DNAJ|Rv0352, the ATPase activity of DNAK|Rv0350. HELPS to release ADP from DNAK thus allowing DNAK to recycle more efficiently. Seems to be regulated negatively by HSPR (Rv0353 product). Probable GrpE protein (HSP-70 cofactor) Rv0351, (MTCY13E10.11), len: 235 aa. Probable grpE protein (HSP-70 cofactor), equivalent to CAC32012.1|AL583925 Hsp70 cofactor from Mycobacterium leprae (229 aa). Also highly similar to others eg Q05562|GRPE_STRCO|2127521|PN0643 GRPE protein from Streptomyces coelicolor (225 aa). Contains grpE protein signature (PS01071). Belongs to the GrpE family. P9WMT5 No virulence, detoxification, adaptation P32724 P9WMT5 GO:0051087,GO:0005739,GO:0006457,GO:0042803,GO:0030150,GO:0006950,GO:0000774 P9WMT5 NC_000962.3 Mycobrowser_v5 CDS 392696 394045 . - 0 Rv0327c cyp135A1 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Possible cytochrome P450 135A1 Cyp135A1 Rv0327c, (MT0342, MTCY63.32c), len: 449 aa. Possible cyp135A1, cytochrome P450, similar to cytochrome P-450 monoxygenases and other cytochrome P-450 related enzymes e.g. FQ12609 putative P450 monooxygenase (506 aa), FASTA scores: opt: 276, E() : 1.7e-11, (27.9% identity in 433 aa overlap). Also similar to other Mycobacterium tuberculosis proteins e.g. MTV039.06|Rv0568 putative cytochrome P450 (472 aa); MTCI5.10 cytochrome p450 FASTA score: (30.4% identity in 434 aa overlap). Contains cytochrome P450 cysteine heme-iron ligand signature (PS00086). Belongs to the cytochrome P450 family. Alternative start possible at 33706 but no RBS. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WPN1 No intermediary metabolism and respiration O08447 P9WPN1 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPN1 NC_000962.3 Mycobrowser_v5 CDS 394111 394713 . + 0 Rv0328 Rv0328 Possibly involved in a transcriptional mechanism. Possible transcriptional regulatory protein (possibly TetR/AcrR-family) Rv0328, (MTCY63.33), len: 200 aa. Possible transcription regulator, TetR/acrR family, similar in part to various hypothetical transcriptional regulators e.g. T36696|4726006|CAB41735.1|AL049731 probable regulatory protein from Streptomyces coelicolor (197 aa). Also some similarity with YX44_MYCTU|Q10829 hypothetical transcriptional regulator from Mycobacterium tuberculosis (195 aa), FASTA scores: opt: 154, E(): 0.00061, (26.7% identity in 202 aa overlap). Contains probable helix-turn helix motif from aa 27-48 (Score 1408, +3.98 SD). Seems to belong to the TetR/AcrR family of transcriptional regulators. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07252 No regulatory proteins O07252 O07252 GO:0006350,GO:0003700,GO:0006355 O07252 NC_000962.3 Mycobrowser_v5 CDS 754980 755225 . - 0 Rv0660c mazE2 Function unknown Possible antitoxin MazE2 Rv0660c, (MTCI376.16), len: 81 aa. Possible mazE2, antitoxin, part of toxin-antitoxin (TA) operon with Rv0659c (See Pandey and Gerdes, 2005; Zhu et al., 2006), showing some similarity to AF016485_130 from Halobacterium sp (100 aa), FASTA scores: (32.4% identity in 74 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06779 No virulence, detoxification, adaptation O06779 O06779 NC_000962.3 Mycobrowser_v5 CDS 394694 395320 . - 0 Rv0329c Rv0329c Function unknown Conserved hypothetical protein Rv0329c, (MTCY63.34c), len: 208 aa. Conserved hypothetical protein, showing some similarity with others hypothetical proteins and methyltransferases e.g. MitM|AF127374_14 methyltransferase from Streptomyces lavendulae (283 aa), FASTA scores: opt: 242, E(): 1.8e-08, (37.2% identity in 145 aa overlap); Q48938 from Methanosarcina barkeri (262 aa), FASTA scores: opt: 194, E(): 3.6e-06, (31.1% identity in 119 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07253 No conserved hypotheticals O07253 O07253 GO:0008168,GO:0008152 O07253 NC_000962.3 Mycobrowser_v5 CDS 395347 396087 . - 0 Rv0330c Rv0330c Unknown Hypothetical protein Rv0330c, (MTCY63.35c), len: 246 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07254 No conserved hypotheticals O07254 O07254 GO:0006350,GO:0003700,GO:0006355 NC_000962.3 Mycobrowser_v5 CDS 1240187 1240885 . + 0 Rv1115 Rv1115 Unknown Possible exported protein Rv1115, (MTCY22G8.04), len: 232 aa. Possible exported protein, contains possible N-terminal signal sequence. O06567 No cell wall and cell processes O06567 O06567 NC_000962.3 Mycobrowser_v5 CDS 4387365 4387895 . - 0 Rv3902c Rv3902c Unknown Hypothetical protein Rv3902c, (MTCY15F10.10), len: 176 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O05443 No conserved hypotheticals O05443 O05443 NC_000962.3 Mycobrowser_v5 CDS 399535 400050 . - 0 Rv0335c PE6 Function unknown PE family protein PE6 Rv0335c, (MTCY279.02c), len: 171 aa. PE6, Member of the Mycobacterium tuberculosis PE family (see Brennan & Delogu 2002); contains short region of similarity to part of the unique N-terminus of the Mycobacterium tuberculosis PGRS family of Glycine-rich proteins e.g. Y03A_MYCTU|Q10637 hypothetical glycine-rich 49.6 kd protein (603 aa), FASTA scores: opt: 219, E(): 1.1e-08, (51.5% identity in 66 aa overlap). L7N648 No PE/PPE O86338 L7N648 L7N648 NC_000962.3 Mycobrowser_v5 CDS 400192 401703 . + 0 Rv0336 Rv0336 Function unknown Conserved 13E12 repeat family protein Rv0336, (MTCY279.03), len: 503 aa. Part of Mycobacterium tuberculosis 13E12 repeat family; almost identical to Rv0515|MTCY20G10.05 hypothetical protein from Mycobacterium tuberculosis FASTA scores: (99.8% identity in 503 aa overlap), possibly due to a recent gene duplication. Also similar to other Mycobacterium tuberculosis hypothetical proteins e.g. Rv1148c, Rv1945, etc. O33266 No insertion seqs and phages O33266 O33266 GO:0003676,GO:0004519 NC_000962.3 Mycobrowser_v5 CDS 401873 403162 . - 0 Rv0337c aspC Generates oxaloacetate and L-glutamate from L-aspartate and 2-oxoglutarate [catalytic activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate]. Probable aspartate aminotransferase AspC (transaminase A) (ASPAT) Rv0337c, (MTCY279.04c), len: 429 aa. Probable aspC, aspartate aminotransferase (transaminase A), equivalent to CAC32019.1|AL583925 probable aspartate aminotransferase from Mycobacterium leprae (437 aa). Also highly similar to many e.g. Q48143|U32823 aspartate aminotransferase (404 aa), FASTA scores: opt: 1646, E(): 0, (57.2% identity in 404 aa overlap). Also some similarity to Rv3565|MTCY06G11.12 from Mycobacterium tuberculosis FASTA score: (27.2% identity in 383 aa overlap). Belongs to class-I of pyridoxal-phosphate-dependent aminotransferases. Cofactor: pyridoxal phosphate. P9WQ91 No intermediary metabolism and respiration P63498 P9WQ91 GO:0004069,GO:0009058,GO:0005737,GO:0030170,GO:0016847 2.6.1.1 P9WQ91 NC_000962.3 Mycobrowser_v5 CDS 443067 444197 . - 0 Rv0365c Rv0365c Function unknown: may be involved in the ability to survive in macrophages. Conserved protein Rv0365c, (MTCY13E10.27c), len: 376 aa (start uncertain). Conserved protein (see citation below), very similar to G388212|CAA35191.1, a truncated ORF immediately upstream of the Corynebacterium glutamicum fda gene encoding fructose-1,6-biphosphate aldolase (304 aa), FASTA scores: E(): 7.1e-19, (42.2% identity in 296 aa overlap). O06315 No conserved hypotheticals O06315 O06315 GO:0003824 O06315 NC_000962.3 Mycobrowser_v5 CDS 2139076 2139687 . - 0 Rv1890c Rv1890c Unknown Hypothetical protein Rv1890c, (MTCY180.28), len: 203 aa. Hypothetical unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). O07742 No conserved hypotheticals O07742 O07742 O07742 NC_000962.3 Mycobrowser_v5 CDS 408634 409173 . + 0 Rv0340 Rv0340 Function unknown Conserved protein Rv0340, (MTCY279.07), len: 179 aa. Conserved protein; MEME-mast analysis shows similarity to product of downstream gene, Rv0341|iniB. O33270 No conserved hypotheticals O33270 EMB,INH NC_000962.3 Mycobrowser_v5 CDS 410838 412760 . + 0 Rv0342 iniA Unknown Isoniazid inductible gene protein IniA Rv0342, iniA, (MTCY13E10.02), len: 640 aa. IniA, isoniazid-inducible gene, (see citations below). Shows slight similarity to some hypothetical bacterial proteins e.g. P40983|YOR6_THER hypothetical protein (402 aa), FASTA scores: opt: 242, E(): 1.4e-07, (22.3% identity in 349 aa overlap). Also some similarity to downstream ORF Rv0343|iniC. Possible transmembrane stretch around residue 490. Alternative start site exists at 410824. Contains a phosphopantetheine attachment site motif suggestive of an acyl carrier protein. Note that the iniA gene is also induced by the antibiotic ethambutol, an agent that inhibits cell wall biosynthesis by a mechanism that is distinct from isoniazid. P9WJ99 No cell wall and cell processes O06293 P9WJ99 GO:0003924,GO:0016021,GO:0005886,GO:0046677,GO:0005525 EMB,INH P9WJ99 NC_000962.3 Mycobrowser_v5 CDS 436860 437507 . + 0 Rv0358 Rv0358 Function unknown Conserved protein Rv0358, (MTCY13E10.20), len: 215 aa. Conserved protein, highly similar to ML0281|AL023514|MLCB4_14 conserved hypothetical protein from Mycobacterium leprae (229 aa), FASTA scores: opt: 852, E(): 0, (62.9% identity in 229 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06308 No conserved hypotheticals O06308 NC_000962.3 Mycobrowser_v5 CDS 444222 444815 . - 0 Rv0366c Rv0366c Function unknown Conserved hypothetical protein Rv0366c, (MTV036.01c), len: 197 aa. Conserved hypothetical protein, showing weak similarity to HI1395|P44173|YD95_HAEIN hypothetical protein from Haemophilus influenzae (140 aa), FASTA scores: opt: 152, E(): 0.0015, (27.0% identity in 126 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS00850 Glycine radical signature. O53701 No conserved hypotheticals O53701 O53701 GO:0001906,GO:0005524 O53701 NC_000962.3 Mycobrowser_v5 CDS 456915 457823 . - 0 Rv0381c Rv0381c Unknown Hypothetical protein Rv0381c, (MTV036.16c), len: 302 aa. Hypothetical unknown protein. Equivalent to AAK44616.1 from Mycobacterium tuberculosis strain CDC1551 (254 aa) but longer 48 aa. O53716 No conserved hypotheticals O53716 NC_000962.3 Mycobrowser_v5 CDS 1184015 1184878 . + 0 Rv1061 Rv1061 Function unknown Conserved protein Rv1061, (MTV017.14), len: 287 aa. Conserved protein, similar to hypothetical proteins from various bacteria e.g. D64002|SYCSLRD_75 Synechocystis sp. PCC6803 (304 aa),FASTA scores: opt: 245, E():1.2e-09, (27.1% identity in 258 aa overlap). O53409 No conserved hypotheticals O53409 O53409 GO:0006541,GO:0009113,GO:0004044 NC_000962.3 Mycobrowser_v5 CDS 3131770 3132714 . - 0 Rv2824c Rv2824c Unknown Hypothetical protein Rv2824c, (MTCY16B7.18), len: 314 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WPJ1 No conserved hypotheticals P71628 P9WPJ1 P9WPJ1 NC_000962.3 Mycobrowser_v5 CDS 414381 414941 . - 0 Rv0344c lpqJ Unknown Probable lipoprotein LpqJ Rv0344c, (MTCY13E10.04c), len: 186 aa. Probable lipoprotein, without homology. Has an appropriately positioned prokaryotic lipoprotein signature (PS00013). O06295 No cell wall and cell processes O06295 NC_000962.3 Mycobrowser_v5 CDS 415050 415460 . + 0 Rv0345 Rv0345 Function unknown Conserved hypothetical protein Rv0345, (MTCY13E10.05), len: 136 aa. Conserved hypothetical protein, similar to other hypothetical proteins e.g. AL13282 4|SCAH10_9 hypothetical protein from Streptomyces coelicolor (207 aa), FASTA scores: opt: 188, E(): 1.5e-05, (41.0% identity in 117 aa overlap). O06296 No conserved hypotheticals O06296 O06296 NC_000962.3 Mycobrowser_v5 CDS 418293 418946 . + 0 Rv0348 Rv0348 Could be involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0348, (MTCY13E10.08), len: 217 aa. Possible transcriptional regulator, showing some similarity to O53334|RV3188|MTV014.32 conserved hypothetical protein from Mycobacterium tuberculosis (115 aa), FASTA score: (30.0% identity in 100 aa overlap). Contains probable helix-turn helix motif from aa 89-110 (Score 1407, +3.98 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06299 No regulatory proteins O06299 O06299 NC_000962.3 Mycobrowser_v5 CDS 498300 499619 . - 0 Rv0412c Rv0412c Unknown Possible conserved membrane protein Rv0412c, (MTCY22G10.08c), len: 439 aa. Possible conserved membrane protein, equivalent to AL035159|MLCB1450_16|T44737 probable membrane protein from Mycobacterium leprae (403 aa), FASTA scores: opt: 2027, E(): 0, (80.4% identity in 403 aa overlap). Also some similarity with CAB71201.1|AL138538 putative secreted protein from Streptomyces coelicolor (429 aa). P96258 No cell wall and cell processes P96258 P96258 NC_000962.3 Mycobrowser_v5 CDS 419835 421712 . + 0 Rv0350 dnaK Acts as a chaperone. Involved in induction by stress conditions e.g. heat shock. Possibly has an ATPase activity. Seems to be regulated positively by sigh (Rv3223c product) and negatively by HSPR (Rv0353 product). Probable chaperone protein DnaK (heat shock protein 70) (heat shock 70 kDa protein) (HSP70) Rv0350, (MTCY13E10.10), len: 625 aa. Probable dnaK (alternate gene name: hsp70), 70 kDa heat shock protein (see citations below), equivalent to AAA25362.1|M95576|1924344A|738248 heat shock protein 70 from Mycobacterium leprae (621 aa); and DNAK_MYCPA|Q00488 (623 aa), FASTA scores: opt: 3678, E(): 0, (92.3% identity in 625 aa overlap). Also highly similar to others e.g. Q05558|DNAK_STRCO|453231|CAA54606.1|X77458 chaperone protein DNAK from Streptomyces coelicolor (618 aa). Has probably an ATPase activity. Note that this sequence differs from DNAK_MYCTU|P32723 (609 aa), due to a frameshift near the N-terminus. Belongs to the heat shock protein 70 family. P9WMJ9 No virulence, detoxification, adaptation P0A5B9 P9WMJ9 GO:0006457,GO:0006950,GO:0051082,GO:0005524 P9WMJ9 NC_000962.3 Mycobrowser_v5 CDS 2307821 2308156 . + 0 Rv2050 Rv2050 Unknown Conserved protein Rv2050, (MTV018.37), len: 111 aa. Conserved protein, similar to many. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WHJ5 No conserved hypotheticals P9WHJ5 P9WHJ5 NC_000962.3 Mycobrowser_v5 CDS 423639 424019 . + 0 Rv0353 hspR Involved in transcriptional regulation (repression) of heat shock proteins e.g. DNAK|Rv0350, GRPE|Rv0351, DNAJ1|Rv0352. Binds to three inverted repeats (IR1-IR3) in the promoter region of the DNAK operon. Induction: by heat shock. Probable heat shock protein transcriptional repressor HspR (MerR family) Rv0353, (MTCY13E10.13), len: 126 aa. Probable hspR, heat shock regulatory protein (see Stewart et al., 2001), merR family, highly similar to others e.g. HspR|P40183 heat shock regulatory protein from Streptomyces coelicolor (151 aa), FASTA scores: E(): 4.9e-22, (55.7% identity in 140 aa overlap), that binds to three inverted repeats (IR1-IR3) in the promoter region of the dnaK operon. Has possible coiled coil region in C-terminal half. Belongs to the MerR family of transcriptional regulators. O06302 No virulence, detoxification, adaptation O06302 O06302 GO:0006355,GO:0006950,GO:0003700,GO:0000166 O06302 NC_000962.3 Mycobrowser_v5 CDS 424777 434679 . - 0 Rv0355c PPE8 Function unknown PPE family protein PPE8 Rv0355c, (MTCY13E10.15c, MTCY13E10.16c, MTCY13E10.17c), len: 3300 aa. PPE8, Member of the Mycobacterium tuberculosis PPE family, similar to others e.g. AL009198|MTV004_5 from Mycobacterium tuberculosis (3716 aa), FASTA scores: opt: 2906, E(): 0, (40.9% identity in 3833 aa overlap); MTV004_3 FASTA scores: (39.0% identity in 3531 aa overlap); etc. Gene contains large number of clustered Major Polymorphic Tandem Repeats (MPTR). Related to MTCY13E10.16c, E(): 0; MTCY13E10.17c, E(): 0; MTCY48.17, E(): 0; MTCY98.0034c, E(): 0; MTCY03C7.23 E(): 0; MTCY98.0031c, E(): 0; MTCY31.06c, E(): 5.6e-17; MTCY359.33, E(): 2.3e-16. Nucleotide position 426909 in the genome sequence has been corrected, A:C resulting in W2591G. I6Y7L4 No PE/PPE Q6MX44 I6Y7L4 NC_000962.3 Mycobrowser_v5 CDS 434830 435474 . - 0 Rv0356c Rv0356c Function unknown Conserved protein Rv0356c, (MTCY13E10.18c), len: 214 aa. Conserved protein, equivalent to AL023514|MLCB4_12 conserved hypothetical protein from Mycobacterium leprae (218 aa), FASTA scores: opt: 1067, E(): 0, (73.4% identity in 214 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06307 No conserved hypotheticals O06307 O06307 O06307 NC_000962.3 Mycobrowser_v5 CDS 437518 438297 . + 0 Rv0359 Rv0359 Unknown Probable conserved integral membrane protein Rv0359, (MTCY13E10.21), len: 259 aa. Probable conserved integral membrane protein, highly similar to hypothetical or other membrane proteins e.g. AL133220|SCC75A_6|T50569 probable membrane protein from Streptomyces coelicolor (265 aa), FASTA scores: opt: 642, E(): 0, (43.1% identity in 248 aa overlap); P70995 hypothetical 24.7 kDa protein from Bacillus subtilis (219 aa), FASTA scores: E(): 1.5e-12, (31.3% identity in 192 aa overlap). Contains neutral zinc metallopeptidases, zinc-binding region signature (PS00142). L0T550 No cell wall and cell processes O06309 L0T550 GO:0006508,GO:0004222 NC_000962.3 Mycobrowser_v5 CDS 438822 439649 . + 0 Rv0361 Rv0361 Unknown Probable conserved membrane protein Rv0361, (MTCY13E10.23), len: 275 aa. Probable conserved membrane protein (has hydrophobic stretch from residues 132-156), equivalent to AL023514|MLCB4_17|AA18949.1|AL023514 putative membrane protein from Mycobacterium leprae (292 aa), FASTA scores: opt: 1044, E(): 0, (58.6% identity in 292 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06311 No cell wall and cell processes O06311 O06311 NC_000962.3 Mycobrowser_v5 CDS 474122 474841 . - 0 Rv0394c Rv0394c Unknown Possible secreted protein Rv0394c, (MTCY04D9.06c), len: 239 aa. Possible secreted protein, sharing no homology with other proteins. Has hydrophobic stretch at its N-terminus. P95202 No cell wall and cell processes P95202 P95202 NC_000962.3 Mycobrowser_v5 CDS 791831 792160 . + 0 Rv0692 Rv0692 Function unknown Conserved hypothetical protein Rv0692, (MTCY210.09), len: 109 aa. Conserved hypothetical protein, highly similar to U17129|RSU17129_3|AAC77477.1 unknown protein from Rhodococcus erythropolis (95 aa), FASTA scores: opt: 393, E(): 8.8e-22, (68.2% identity in 88 aa overlap). P95038 No conserved hypotheticals P95038 NC_000962.3 Mycobrowser_v5 CDS 441265 442299 . - 0 Rv0363c fba Involved in glycolysis (at the sixth step) [catalytic activity: D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate]. Probable fructose-bisphosphate aldolase Fba Rv0363c, (MTCY13E10.25c), len: 344 aa. Probable fba (alternate gene name: fda), fructose bisphosphate aldolase , equivalent to AL023514|MLCB4_18|O69600|ALF_MYCLE fructose-bisphosphate aldolase from Mycobacterium leprae (345 aa), FASTA scores: opt: 1995, E(): 0, (87.7% identity in 342 aa overlap). Also highly similar to others. Belongs to class II fructose-bisphosphate aldolase family. Cofactor: zinc. P9WQA3 No intermediary metabolism and respiration 3EKL,3EKZ,3ELF,4A21,4A22 P67475 P9WQA3 GO:0006096,GO:0008270,GO:0004332 4.1.2.13 P9WQA3 NC_000962.3 Mycobrowser_v5 CDS 446531 447046 . - 0 Rv0369c Rv0369c Function unknown; probably involved in cellular metabolism. Possible membrane oxidoreductase Rv0369c, (MTV036.04c), len: 171 aa. Possible membrane protein oxidoreductase, similar to ORF 4 of the Pseudomonas thermocarboxydovorans protein of cutA-cutB-cutC gene cluster: X77931|PTC2CUTAC_4 ORF4 from Pseudomonas thermocarboxydovorans (171 aa), FASTA scores: opt: 226, E(): 9.8e-08, (31.3% identity in 166 aa overlap). Also similar to MTV036.05, MTV036.08, MTV036.09, and MTV026.10. O53704 No intermediary metabolism and respiration O53704 O53704 GO:0016491,GO:0055114 1.-.-.- O53704 NC_000962.3 Mycobrowser_v5 CDS 447147 448043 . - 0 Rv0370c Rv0370c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0370c, (MTV036.05c), len: 298 aa. Possible oxidoreductase, similar to many hypothetical proteins, but also similar to ORF4|X82447|OCCOXMSL4_4 Protein of coxMSL gene cluster from Pseudomonas/Oligotropha carboxidovorans (295 aa), FASTA scores: opt: 851, E(): 0, (48.2% identity in 282 aa overlap); AJ224684|BJAJ4684_3 cooxS from Bradyrhizobium japonicum (302 aa), FASTA scores: opt: 881, E(): 0, (47.6% identity in 290 aa overlap). Also highly similar to MTCY428_21 from Mycobacterium tuberculosis. Contains PS00017 ATP/GTP-binding site motif A (P-loop). O53705 No intermediary metabolism and respiration O53705 O53705 GO:0016887,GO:0055114,GO:0016491,GO:0005524 1.-.-.- NC_000962.3 Mycobrowser_v5 CDS 532396 532911 . + 0 Rv0443 Rv0443 Function unknown Conserved protein Rv0443, (MTV037.07), len: 171 aa. Conserved protein, highly similar to AL049863|SC5H1_23|T35339 hypothetical protein from Streptomyces coelicolor (171 aa), FASTA scores: opt: 561, E(): 2.3e-32, (49.7% identity in 165 aa overlap); and CAC42482.1|AJ318385 hypothetical protein from Amycolatopsis mediterranei (163 aa). O53728 No conserved hypotheticals O53728 O53728 O53728 NC_000962.3 Mycobrowser_v5 CDS 1589386 1589901 . + 0 Rv1413 Rv1413 Function unknown Conserved hypothetical protein Rv1413, (MTCY21B4.30), len: 171 aa. Conserved hypothetical protein, similar to part of AB010956|AB010956_1 metal-activated pyridoxal enzyme from Arthrobacter sp. (379 aa), FASTA scores: opt: 187, E(): 0.00026, (29.0% identity in 162 aa overlap). P9WLY5 No conserved hypotheticals P64843 P9WLY5 P9WLY5 NC_000962.3 Mycobrowser_v5 CDS 451800 452279 . - 0 Rv0374c Rv0374c Function unknown; probably involved in cellular metabolism [catalytic activity: CO + H(2)O + acceptor = CO(2) + reduced acceptor]. Probable carbon monoxyde dehydrogenase (small chain) Rv0374c, (MTV036.09c), len: 159 aa. Probable carbon monoxide dehydrogenase, small chain, highly similar to others e.g. B56279|5822285|X82447|OCCOXMSL4_2|COXS carbon-monoxide dehydrogenase small chain from Pseudomonas/Oligotropha carboxydovorans (166 aa), FASTA scores: opt: 662, E(): 0, (59.3% identity in 150 aa overlap); CAA12063.1|AJ224684 putative carbon monoxide dehydrogenase small subunit from Bradyrhizobium japonicum (161 aa); S49123|509390|CAA54901.1|X77931|CUTC carbon-monoxide dehydrogenase small chain from Pseudomonas thermocarboxydovorans (163 aa); etc. O53709 No intermediary metabolism and respiration O53709 O53709 GO:0009055,GO:0051536,GO:0046872,GO:0055114,GO:0018492 1.2.99.2 O53709 NC_000962.3 Mycobrowser_v5 CDS 452294 453154 . - 0 Rv0375c Rv0375c Function unknown; probably involved in cellular metabolism [catalytic activity: CO + H(2)O + acceptor = CO(2) + reduced acceptor]. Probable carbon monoxyde dehydrogenase (medium chain) Rv0375c, (MTV036.10c), len: 286 aa. Probable carbon monoxide dehydrogenase, medium chain, similar to others e.g. AAD00361.1|U80806|CUTM carbon monoxide dehydrogenase middle subunit from Hydrogenophaga pseudoflava (287 aa); S49122|509389|CAA54900.1|X77931|CUTB carbon-monoxide dehydrogenase medium chain from Pseudomonas thermocarboxydovorans (287 aa); A56279|809564|CAA57827.1|X82447|OCCOXMSL4_1|COXM|CODH carbon-monoxide dehydrogenase medium chain from Pseudomonas/Oligotropha carboxydovorans (288 aa), FASTA scores: opt: 594, E(): 0, (37.5% identity in 277 aa overlap); etc. I6Y7N2 No intermediary metabolism and respiration O53710 I6Y7N2 GO:0018492,GO:0055114,GO:0050660 1.2.99.2 I6Y7N2 NC_000962.3 Mycobrowser_v5 CDS 466672 467406 . - 0 Rv0387c Rv0387c Function unknown Conserved hypothetical protein Rv0387c, (MTV036.22c), len: 244 aa. Conserved hypothetical protein, showing some similarity to MTCI237.20c, and M17282|HUMEL20_1 Human elastin gene, exon 1, Elastin (687 aa), FASTA scores: opt: 193, E(): 0.35, (34.4% identity in 189 aa overlap). L0T6I4 No conserved hypotheticals O53721 L0T6I4 NC_000962.3 Mycobrowser_v5 CDS 476679 477320 . - 0 Rv0398c Rv0398c Unknown Possible secreted protein Rv0398c, (MTCY04D9.10c), len: 213 aa. Possible secreted protein, sharing no homology with other proteins. Has potential signal sequence with hydrophobic stretch from aa 7-25. P95206 No cell wall and cell processes P95206 NC_000962.3 Mycobrowser_v5 CDS 455977 456192 . + 0 Rv0379 secE2 Thought to be involved in protein transport (export). Possible protein transport protein SecE2 Rv0379, (MTV036.14), len: 71 aa. Possible secE2, protein transport protein, showing similarity with P27340|S61G_SULSO|SECE preprotein translocase SECE subunit (protein transport protein SEC61 gamma subunit homolog) from Sulfolobus acidocaldarius (65 aa), FASTA scores: opt: 79, E(): 4.7. (30.3% identity in 66 aa overlap); and hypothetical proteins e.g. Q9HPW4|VNG1446H hypothetical protein from Halobacterium sp. strain NRC-1 (77 aa); Q9I794|PA0038 hypothetical protein from Pseudomonas aeruginosa (71 aa); etc. Also highly similar to U85467|MTU85467_1 hypothetical Mycobacterium tuberculosis protein from a patient isolate (116 aa), FASTA scores: opt: 443, E(): 7.7e-29, (98.6% identity in 71 aa overlap). Note that for Rv0379|MTV036.14, a translation initiation region different to the one in U85467|MTU85467_1 was chosen. Could be a part of the prokaryotic protein translocation apparatus which comprise SECA|Rv3240c, SECD|Rv2587c, SECE|Rv0638, SECF|Rv2586c, SECG|Rv1440 and SECY|Rv0732. Q6MX43 No cell wall and cell processes 3ONR Q6MX43 Q6MX43 Q6MX43 NC_000962.3 Mycobrowser_v5 CDS 456268 456819 . - 0 Rv0380c Rv0380c Possibly causes methylation of RNA. Possible RNA methyltransferase (RNA methylase) Rv0380c, (MTV036.15c), len: 183 aa. Possible RNA methyltransferase, equivalent to CAC32002.1|AL583925 possible RNA methyltransferase from Mycobacterium leprae (182 aa). Also some similarity with others methyltransferases e.g. P19396|TRMH_ECOLI|78514|JV0043 tRNA (guanosine-2'-O-)-methyltransferase (tRNA methyltransferase) from Escherichia coli (229 aa), FASTA scores: opt: 227, E(): 1.4e-09, (28.9% identity in 166 aa overlap). Also similar to Rv0881, Rv3579c, Rv1644 from Mycobacterium tuberculosis. O53715 No intermediary metabolism and respiration O53715 O53715 GO:0008173,GO:0006396,GO:0003723 2.1.1.- O53715 NC_000962.3 Mycobrowser_v5 CDS 467459 468001 . - 0 Rv0388c PPE9 Function unknown PPE family protein PPE9 Rv0388c, (MTV036.23c), len: 180 aa. PPE9, Member of the Mycobacterium tuberculosis PPE family, highly similar to others e.g. MTCY10G2_10|Z92539 from Mycobacterium tuberculosis (391 aa), FASTA scores: opt: 667, E(): 0, (58.3% identity in 180 aa overlap) but much shorter. L0T6B3 No PE/PPE Q6MX42 L0T6B3 L0T6B3 NC_000962.3 Mycobrowser_v5 CDS 566776 567222 . + 0 Rv0477 Rv0477 Unknown Possible conserved secreted protein Rv0477, (MTCY20G9.03), len: 148 aa. Possible conserved secreted protein, equivalent to CAC31969.1|AL583925 hypothetical protein from Mycobacterium leprae (123 aa). Also similar to G83406|PA1914 conserved hypothetical protein from Pseudomonas aeruginosa (408 aa). Contains possible N-terminal signal sequence. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WKV9 No cell wall and cell processes P64697 P9WKV9 NC_000962.3 Mycobrowser_v5 CDS 2447605 2447994 . - 0 Rv2186c Rv2186c Unknown Conserved hypothetical protein Rv2186c, (MTV021.19c), len: 129 aa. Conserved hypothetical protein, equivalent to hypothetical Mycobacterium leprae protein ML0888 (135 aa). FASTA scores: ML0888 opt: 704, E(): 2.9e-43; 80.000% identity in 130 aa overlap CAA18694.1| (AL022602). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53520 No conserved hypotheticals O53520 NC_000962.3 Mycobrowser_v5 CDS 458461 459315 . - 0 Rv0383c Rv0383c Unknown Possible conserved secreted protein Rv0383c, (MTV036.18c), len: 284 aa. Possible conserved secreted protein, with hydrophobic stretch in N-terminus and Pro-rich C-terminus. Equivalent to CAC32006.1|AL583925 possible secreted protein from Mycobacterium leprae (286 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53718 No cell wall and cell processes O53718 NC_000962.3 Mycobrowser_v5 CDS 462135 463307 . + 0 Rv0385 Rv0385 Function unknown; probably involved in cellular metabolism. Probable monooxygenase Rv0385, (MTV036.20), len: 390 aa. Probable monooxygenase, similar to T37003|5738846|CAB52917.1|AL109949 probable flavohemoprotein from Streptomyces coelicolor (435 aa); and similar in part (C-termini) to various monooxygenases e.g. P19734|DMPP_PSESP|94993|F37831 phenol hydroxylase P5 protein (phenol 2-monooxygenase P5 component) from Pseudomonas putida (353 aa), FASTA scores: opt: 363, E(): 4.2e-16, (31.8% identity in 255 aa overlap); S47292|2120861|pir|S70085 phenol 2-monooxygenase chain mopP from Acinetobacter calcoaceticus (350 aa); P21394|XYLA_PSEPU|94933|B37316 xylene monooxygenase electron transfer component [includes: ferredoxin; ferredoxin--NAD(+) reductase] from Pseudomonas putida plasmid pWW0 (350 aa); AAC38360.1|AF043544|NtnMA|ntnA reductase component of 4-nitrotoluene monooxygenase from Pseudomonas sp. (328 aa); etc. Q7ARS9 No intermediary metabolism and respiration Q7ARS9 Q7ARS9 GO:0004497,GO:0055114,GO:0019825,GO:0015671,GO:0005344,GO:0020037 1.-.-.- Q7ARS9 NC_000962.3 Mycobrowser_v5 CDS 463411 466668 . + 0 Rv0386 Rv0386 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably LuxR/UhpA-family) Rv0386, (MTV036.21), len: 1085 aa. Probable regulatory protein, LuxR/uhpA family, highly similar to CAC30706.1|AL583923 possible transcriptional regulator from Mycobacterium leprae (1106 aa). Also similar in part to other regulatory proteins e.g. CAB95788.1|AL359949 putative multi-domain regulatory protein from Streptomyces coelicolor (780 aa); N-terminus of CAB92369.1|AL356612 putative AfsR-like regulatory protein from Streptomyces coelicolor (1114 aa); N-terminus of NP_107139.1|14026327|BAB52925.1|AP003009 transcriptional regulator from Mesorhizobium loti (952 aa); AFSR_STRCO|P25941 regulatory protein afsr from Streptomyces coelicolor (993 aa), FASTA scores: opt: 224, E() : 1.1e-06, (26.1% identity in 867 aa overlap); etc. Also similar to many putative Mycobacterium tuberculosis regulatory proteins e.g. AL0212|MTV008_44 (1137 aa), FASTA scores: opt: 3756, E(): 0, (56.7% identity in 1089 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00622 Bacterial regulatory proteins, luxR family signature and probable helix-turn-helix motif at aa 1042-1063 (Score 1025, +2.68 S D). Belongs to the LuxR/UhpA family of transcriptional regulators. O53720 No regulatory proteins O53720 O53720 GO:0006915,GO:0009190,GO:0006952,GO:0005622,GO:0016849,GO:0006355,GO:0043565,GO:0003700,GO:0000156,GO:0000160,GO:0005524 O53720 NC_000962.3 Mycobrowser_v5 CDS 1241003 1241188 . + 0 Rv1116 Rv1116 Unknown Hypothetical protein Rv1116, (MTCY22G8.05), len: 61 aa. Hypothetical unknown protein. O06568 No conserved hypotheticals O06568 NC_000962.3 Mycobrowser_v5 CDS 470010 471230 . + 0 Rv0391 metZ Involved in methionine biosynthesis. Converts O-succinylhomoserine into homocysteine. Probable O-succinylhomoserine sulfhydrylase MetZ (OSH sulfhydrylase) Rv0391, (MTCY04D9.03), len: 406 aa. Probable metZ, O-succinylhomoserine sulfhydrylase, equivalent, but shorter 20 aa in N-terminus, to AA18941.1|AL023514 O-succinylhomoserine sulfhydrylase from Mycobacterium leprae (426 aa). Also highly similar to others e.g. METZ_PSEAE|P55218 o-succinylhomoserine sulfhydrylase from Pseudomonas aeruginosa (403 aa), FASTA scores: opt: 1175, E(): 0, (47.2% identity in 392 aa overlap); etc. Belongs to the trans-sulfuration enzymes family. Could also be a cystathionine gamma-synthase. P9WGB5 No intermediary metabolism and respiration 3NDN P95199 P9WGB5 GO:0016829,GO:0030170,GO:0006520 4.2.99.- P9WGB5 NC_000962.3 Mycobrowser_v5 CDS 471227 472639 . - 0 Rv0392c ndhA Transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Does not couple the redox reaction to proton translocation [catalytic activity: NADH + acceptor = NAD+ + reduced acceptor]. Probable membrane NADH dehydrogenase NdhA Rv0392c, (MTCY04D9.04c), len: 470 aa. Probable ndhA, membrane NADH dehydrogenase, equivalent to many e.g. AF038423|AF038423_1 NADH dehydrogenase from Mycobacterium smegmatis (457 aa), FASTA scores: opt: 1991, E(): 0, (67.9% identity in 458 aa overlap); MLCB1788_3 NADH dehydrogenase from Mycobacterium leprae (466 aa), FASTA score: (62.5% identity in 467 aa overlap). Also similar to others from several organisms e.g. P00393|DHNA_ECOLI|66211|581140|CAA23586.1|V00306 NADH dehydrogenase from Escherichia coli (434 aa); and Rv0392c|ndhB from Mycobacterium tuberculosis. Has hydrophobic stretch in C-terminus. Belongs to the NADH dehydrogenase family. P95200 No intermediary metabolism and respiration P95200 P95200 GO:0003954,GO:0055114,GO:0050660 1.6.99.3 P95200 NC_000962.3 Mycobrowser_v5 CDS 477327 478556 . - 0 Rv0399c lpqK Unknown Possible conserved lipoprotein LpqK Rv0399c, (MTCY04D9.11c), len: 409 aa. Possible lpqK, conserved lipoprotein, showing some similarity to penicillin binding proteins and various peptidases e.g. DAC_STRSQ|P15555 d-alanyl-d-alanine carboxypeptidase protein (406 aa), FASTA scores: opt: 348, E(): 5.6e-16, (29.2% identity in 301 aa overlap). Also similar to other Mycobacterium tuberculosis PBPs and esterases. Has possible N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein lipid attachment site (PS00013). P95207 No cell wall and cell processes P95207 P95207 P95207 NC_000962.3 Mycobrowser_v5 CDS 544835 545185 . + 0 Rv0454 Rv0454 Function unknown Conserved hypothetical protein Rv0454, (MTV037.18), len: 116 aa (start uncertain). Conserved hypothetical protein, showing similarity with AAA63007.1|U15183 hypothetical protein from Mycobacterium leprae (115 aa), FASTA scores: opt: 151, E(): 0.0019, (31.5% identity in 89 aa overlap). O86364 No conserved hypotheticals O86364 NC_000962.3 Mycobrowser_v5 CDS 480355 483231 . - 0 Rv0402c mmpL1 Unknown. Thought to be involved in fatty acid transport. Probable conserved transmembrane transport protein MmpL1 Rv0402c, (MTCY04D9.15c), len: 958 aa. Probable mmpL1, conserved transmembrane transport protein (see Tekaia et al., 1999), member of RND superfamily, highly similar to other Mycobacterial proteins e.g. YV34_MYCTU|Q11171 hypothetical 106.2 kDa membrane protein from Mycobacterium tuberculosis (968 aa), FASTA scores: opt: 3551, E(): 0, (55.4% identity in 933aa overlap); YV34_MYCLE|P54881 hypothetical 105.2 kDa protein from Mycobacterium leprae (959 aa), FASTA scores: opt: 3615, E(): 0, (55.5% identity in 941 aa overlap); etc. Highly similar to many other mycobacterial MmpL proteins from Mycobacterium tuberculosis and Mycobacterium leprae e.g. Rv0450c, Rv0676c, Rv0507, etc. Belongs to the MmpL family. P9WJV9 No cell wall and cell processes P95211 P9WJV9 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 483228 483656 . - 0 Rv0403c mmpS1 Unknown Probable conserved membrane protein MmpS1 Rv0403c, (MTCY04D9.16c), len: 142 aa. Probable mmpS1, conserved membrane protein (see citation below), highly similar to other Mycobacterial proteins e.g. YV33_MYCLE|P54880 hypothetical 16.9 kDa protein from Mycobacterium leprae (154 aa), FASTA scores: opt: 458, E(): 1.6e-26, (46.9% identity in 143 aa overlap); YV33_MYCTU|Q11170 hypothetical 15.9 kDa protein from Mycobacterium tuberculosis (147 aa), FASTA scores: opt: 362, E(): 1.1e-19, (42.1% identity in 140 aa overlap); etc. Also similar to other MmpS proteins from Mycobacterium tuberculosis e.g. Rv0677c, Rv0451c, etc. Belongs to the MmpS family. Predicted to be an outer membrane protein (See Song et al., 2008). P9WJT5 No cell wall and cell processes P0A5K0 P9WJT5 GO:0005886,GO:0016021 P9WJT5 NC_000962.3 Mycobrowser_v5 CDS 799629 799850 . + 0 Rv0699 Rv0699 Unknown Hypothetical protein Rv0699, (MTCY210.17), len: 73 aa. Hypothetical unknown protein. P95046 No conserved hypotheticals P95046 P95046 NC_000962.3 Mycobrowser_v5 CDS 485731 489939 . + 0 Rv0405 pks6 Polyketide synthase possibly involved in lipid synthesis. Probable membrane bound polyketide synthase Pks6 Rv0405, (MTCY22G10.01), len: 1402 aa. Probable pks6, membrane-bound polyketide synthase (see citation below), highly similar to others e.g. CAC29643.1|AL583917 putative polyketide synthase from Mycobacterium leprae (2103 aa); Y06K_MYCTU|Q10977 probable polyketide synthase (1876 aa), FASTA scores: opt: 2303, E(): 0, (38.7% identity in 1232 aa overlap); etc. Contains PS00606 Beta-ketoacyl synthases active site, 2 x PS00017 ATP/GTP-binding site motif A (P-loop), and PS00012 Phosphopantetheine attachment site. O86335 No lipid metabolism O86335 O86335 GO:0009058,GO:0048037,GO:0016788,GO:0031177,GO:0016740,GO:0000036 O86335 NC_000962.3 Mycobrowser_v5 CDS 489887 490705 . - 0 Rv0406c Rv0406c Function unknown Beta lactamase like protein Rv0406c, (MTCY22G10.02c), len: 272 aa. Beta-lactamase-like protein, equivalent to AAD38170.1|AF152397_1 beta-lactamase-like protein from Mycobacterium phlei (243 aa); AL023514|MLCB4_8 hypothetical protein from Mycobacterium leprae (251 aa), FASTA scores: opt: 1284, E(): 0, (74.9% identity in 243 aa overlap); and AAD38164.1|AF152394_2 beta-lactamase-like protein from Mycobacterium avium (247 aa), FASTA scores: opt: 1301, E(): 0, (74.2% identity in 244 aa overlap); etc. Also slight similarity to others beta-lactamases and hypothetical proteins e.g. P52700|BLA1_XANMA|628530|S45349 metallo-beta-lactamase L1 precursor (beta-lactamase, type II) (penicillinase) from Xanthomonas maltophilia (290 aa), FASTA scores: (34.4% identity in 96 aa overlap). Recombinant protein has beta lactamase activity (See Nampoothiri et al., 2008). O86336 No conserved hypotheticals O86336 GO:0016787 O86336 NC_000962.3 Mycobrowser_v5 CDS 493851 495008 . + 0 Rv0409 ackA Involved at the first step (of two) in the conversion of acetate to acetyl-CoA [catalytic activity: ATP + acetate = ADP + acetyl phosphate]. Probable acetate kinase AckA (acetokinase) Rv0409, (MTCY22G10.05), len: 385 aa. Probable ackA, acetate kinase, highly similar to others e.g. ACKA_BACSU|P37877 acetate kinase from Bacillus subtilis (395 aa), FASTA scores: opt: 974, E(): 0, (43.5% identity in 393 aa overlap); etc. Contains PS01075 Acetate and butyrate kinases family signature 1, PS00758 ArgE / dapE / ACY1/ CPG2 / yscS family signature 1. Belongs to the acetokinase family. P9WQH1 No intermediary metabolism and respiration P63409 P9WQH1 GO:0008776,GO:0005737,GO:0006082,GO:0016310,GO:0005524 2.7.2.1 P9WQH1 NC_000962.3 Mycobrowser_v5 CDS 497314 498300 . - 0 Rv0411c glnH Involved in active transport of glutamine across the membrane (import). Interacts with the glutamine-transport system. Probable glutamine-binding lipoprotein GlnH (GLNBP) Rv0411c, (MTCY22G10.07c), len: 328 aa. Probable glnH, glutamine-binding protein, membrane-bound lipoprotein (see citation below), equivalent to AL035159|MLCB1450_15|T44736|4154051|CAA22704.1 glutamine-binding protein homolog from Mycobacterium leprae (325 aa), FASTA scores: opt: 1747, E(): 0, (79.3% identity in 328 aa overlap). Also similar to others e.g. GLNH_BACST|P27676 glutamine-binding protein precursor from Bacillus stearothermophilus (262 aa), FASTA scores: opt: 493, E(): 7.5e-22, (37.8% identity in 193 aa overlap); etc. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site, PS01039 Bacterial extracellular solute-binding proteins, family 3 signature. Belongs to the bacterial extracellular solute-binding protein family 3. Presumed attached to the membrane by a lipid anchor. P96257 No cell wall and cell processes P96257 P96257 GO:0006810,GO:0005215,GO:0030288 P96257 NC_000962.3 Mycobrowser_v5 CDS 1241633 1241956 . + 0 Rv1117 Rv1117 Function unknown Conserved protein Rv1117, (MTCY22G8.06), len: 107 aa. Conserved protein, some similarity to P94425|D50453 hypothetical protein from Bacillus subtilis (95 aa), fasta scores: opt: 128, E(): 5.1e-06, (28.3% identity in 92 aa overlap); and AL117322|SCF1.02 Streptomyces coelicolor (109 aa), FASTA scores: opt: 437, E(): 1.6e-25, (57.5% identity in 106 aa overlap). O06569 No conserved hypotheticals O06569 O06569 GO:0005737,GO:0016491,GO:0017000 O06569 NC_000962.3 Mycobrowser_v5 CDS 495062 497314 . - 0 Rv0410c pknG Involved in signal transduction (via phosphorylation). Thought to regulate amino-acid uptake and stationary-phase metabolism. Phosphorylates the peptide substrate myelin basic protein (MBP) at serine residues [catalytic activity: ATP + a protein = ADP + a phosphoprotein]. Serine/threonine-protein kinase PknG (protein kinase G) (STPK G) Rv0410c, (MTCY22G10.06c), len: 750 aa. PknG, serine/threonine-protein kinase (see citations below), equivalent to PKNG_MYCLE|P57993|13092623|CAC29812.1|AL583918 probable serine/threonine-protein kinase from Mycobacterium leprae (767 aa). Also similar to others e.g. AB76890.1|AL159139 putative serine/threonine protein kinase from Streptomyces coelicolor (774 aa); etc. Contains PS00108 Serine/Threonine protein kinases active-site signature. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases. Structure of PknG with inhibitor AX20017 reveals that the inhibitor-binding pocket is shaped by a unique set of amino acid side chains not found in any human kinase (See Scherr et al., 2007). P9WI73 No regulatory proteins 2PZI P65728 P9WI73 GO:0006468,GO:0004674,GO:0005524 2.7.11.1 P9WI73 NC_000962.3 Mycobrowser_v5 CDS 1051544 1052317 . - 0 Rv0941c Rv0941c Function unknown Conserved hypothetical protein Rv0941c, (MTCY10D7.33), len: 257 aa. Conserved hypothetical protein, showing some similarity with parts of several hypothetical proteins from Streptomyces coelicolor e.g. AL035161|SC9C7_20 (860 aa), FASTA scores: opt: 197, E(): 2.6e-05, (34.2% identity in 114 aa overlap). P71568 No conserved hypotheticals P71568 P71568 P71568 NC_000962.3 Mycobrowser_v5 CDS 501148 502170 . + 0 Rv0415 thiO Possibly involved in thiamine biosynthesis. Possible thiamine biosynthesis oxidoreductase ThiO Rv0415, (MTCY22G10.12), len: 340 aa. Possible thiO, thiamine biosynthesis oxidoreductase, equivalent to T44739|4154054|CAA22708.1|AL035159|MLCB1450.24 hypothetical protein from Mycobacterium leprae (340 aa), FASTA scores: opt: 1867, E(): 0, (82.0% identity in 338 aa overlap). Shows some similarity to other thiO proteins e.g. THIO_RHIET|O34292 Putative thiamine biosynthesis oxidoreductase from Rhizobium etli plasmid pb (327 aa) (see citation below); AAG31046.1|AF264948_8|THIO putative amino acid oxidase flavoprotein ThiO from Erwinia amylovora (349 aa); NP_106392.1|14025578|BAB52178.1|AP003007|THIO thiamine biosynthesis oxidoreductase THIO from Mesorhizobium loti (333 aa); etc. P96261 No intermediary metabolism and respiration P96261 P96261 GO:0055114,GO:0016491,GO:0050660 1.-.-.- P96261 NC_000962.3 Mycobrowser_v5 CDS 502167 502373 . + 0 Rv0416 thiS Possibly involved in thiamine biosynthesis. Possible protein ThiS Rv0416, (MTCY22G10.13), len: 68 aa. Possible thiS protein, equivalent to T44740|4154055|CAA22709.1|AL035159|MLCB1450.25 hypothetical protein from Mycobacterium leprae (74 aa), FASTA scores: opt: 303, E(): 2e-18, (71.6% identity in 74 aa overlap). Shows weak similarity with O32583|THIS_ECOLI|THIG1|B3991.1 this protein from Escherichia coli strain K12 (66 aa), FASTA scores: opt: 103, E(): 0.052, (30.9% identity in 68 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P96262 No intermediary metabolism and respiration P96262 P96262 GO:0006790 P96262 NC_000962.3 Mycobrowser_v5 CDS 545889 546803 . - 0 Rv0456c echA2 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. enoyl-CoA hydratase EchA2 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv0456c, (MTCI429A.02, MTV037.20c), len: 304 aa. Probable echA2, enoyl-CoA hydratase, similar to other enoyl-CoA hydratases e.g. Q13011 peroxisomal enoyl-CoA hydratase-like protein (328 aa), FASTA scores: opt: 209, E(): 5.3e-07, (31.7% identity in 142 aa overlap). Also similar to several other proteins from Mycobacterium tuberculosis e.g. MTCY09F9.29 FASTA score: (32.9% identity in 146 aa overlap); and MTI376.01c. O07179 No lipid metabolism O07179 O07179 GO:0016853,GO:0008152,GO:0004300 4.2.1.17 O07179 NC_000962.3 Mycobrowser_v5 CDS 1592150 1592614 . + 0 Rv1417 Rv1417 Unknown Possible conserved membrane protein Rv1417, (MTCY21B4.35), len: 154 aa. Possible conserved membrane protein, similar to others e.g. AL133213|SC6D7_2 Streptomyces coelicolor (156 aa), FASTA scores: opt: 212, E(): 4.4e-07, (32.4% identity in 136 aa overlap). P9WLY1 No cell wall and cell processes P64847 P9WLY1 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 505086 506582 . + 0 Rv0419 lpqM Function unknown; possibly hydrolyzes peptides and/or proteins. Possible lipoprotein peptidase LpqM Rv0419, (MTCY22G10.16), len: 498 aa. Possible lpqM, lipoprotein peptidase ; has potential N-terminal signal peptide and contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site, PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. P96265 No cell wall and cell processes P96265 GO:0004177 3.4.11.- P96265 NC_000962.3 Mycobrowser_v5 CDS 506561 506971 . - 0 Rv0420c Rv0420c Unknown Possible transmembrane protein Rv0420c, (MTCY22G10.17c), len: 136 aa. Possible transmembrane protein; has potential transmembrane domains aa 53-99 and aa 100-122. P96266 No cell wall and cell processes P96266 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 507132 507761 . - 0 Rv0421c Rv0421c Function unknown Conserved hypothetical protein Rv0421c, (MTCY22G10.18c), len: 209 aa. Conserved hypothetical protein, showing similarity with NP_103507.1|14022684|BAB49293.1|AP002998 hypothetical protein from Mesorhizobium loti (214 aa). P96267 No conserved hypotheticals P96267 P96267 NC_000962.3 Mycobrowser_v5 CDS 507758 508555 . - 0 Rv0422c thiD Involved in thiamine biosynthesis. Catalyzes the phosphorylation of HMP-P to HMP-pp [catalytic activity: ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine]. Probable phosphomethylpyrimidine kinase ThiD (HMP-phosphate kinase) (HMP-P kinase) Rv0422c, (MTCY22G10.19c), len: 265 aa. Probable thiD, phosphomethylpyrimidine kinase, equivalent to AL035159|MLCB1450_21 phosphomethylpyrimidine kinase from Mycobacterium leprae (279 aa), FASTA scores: opt: 1386, E(): 0, (77.8% identity in 266 aa overlap). Also highly similar to others e.g. HIU32725_3|P44697|THID_HAEIN phosphomethylpyrimidine kinase from Haemophilus influenzae (269 aa), FASTA scores: opt: 605, E(): 0, (42.1% identity in 259 aa overlap). Belongs to the ThiD family. P9WG77 No intermediary metabolism and respiration P66913 P9WG77 GO:0008902,GO:0008972,GO:0009228,GO:0005524 2.7.4.7,2.7.1.49 P9WG77 NC_000962.3 Mycobrowser_v5 CDS 510377 510652 . - 0 Rv0424c Rv0424c Unknown Hypothetical protein Rv0424c, (MTCY22G10.21c), len: 91 aa. Hypothetical unknown protein. P96270 No conserved hypotheticals P96270 P96270 NC_000962.3 Mycobrowser_v5 CDS 596759 597202 . + 0 Rv0506 mmpS2 Unknown Probable conserved membrane protein MmpS2 Rv0506, (MTCY20G9.33), len: 147 aa. Probable mmpS2, conserved membrane protein (see citation below), highly similar to other Mycobacterial proteins e.g. C-terminus of AAD44232.1|AF143772_38|AF143772|TmtpA from Mycobacterium avium (221 aa); P54880|MMS4_MYCLE|MMPS4 putative membrane protein from Mycobacterium leprae (154 aa), FASTA scores: opt: 392, E(): 1.3e-20, (43.7% identity in 151 aa overlap); and the putative membrane proteins from Mycobacterium tuberculosis MTV040_5, MTCY4D9_16, MTV037_15. Belongs to the MmpS family. Predicted to be an outer membrane protein (See Song et al., 2008). P9WJT3 No cell wall and cell processes P65376 P9WJT3 GO:0016021 P9WJT3 NC_000962.3 Mycobrowser_v5 CDS 1592639 1593325 . + 0 Rv1418 lprH Unknown Probable lipoprotein LprH Rv1418, (MTCY21B4.36), len: 228 aa. Probable lprH, lipoprotein. Contains N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein lipid attachment site (PS00013). P9WK43 No cell wall and cell processes P9WK43 GO:0005886,GO:0016021 P9WK43 NC_000962.3 Mycobrowser_v5 CDS 2370598 2370792 . - 0 Rv2111c pup Involved in proteasomal degradation. Covalently binds to protein substrates. Prokaryotic ubiquitin-like protein Pup Rv2111c, MTCY261.07c, len: 64 aa. Pup, prokaryotic ubiquitin-like protein (See Pearce et al., 2008). Highly similar to many. Pup|Rv2111c and Mpa|Rv2115c interact (See Pearce et al., 2008). P9WHN5 No intermediary metabolism and respiration 3M91,3M9D O33246 P9WHN5 GO:0070490,GO:0019941 P9WHN5 NC_000962.3 Mycobrowser_v5 CDS 515373 515816 . - 0 Rv0426c Rv0426c Unknown Possible transmembrane protein Rv0426c, (MTCY22G10.23c), len: 147 aa. Possible transmembrane protein; has potential transmembrane domains aa 19-41, and aa 61-83. P96272 No cell wall and cell processes P96272 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 517803 518396 . - 0 Rv0429c def Removes the formyl group from the N-terminal met of newly synthesized proteins [catalytic activity: N-formyl-L-methionine + H2O = formate + L-methionine]. Probable polypeptide deformylase Def (PDF) (formylmethionine deformylase) Rv0429c, (MTCY22G10.26c), len: 197 aa. Probable def, polypeptide deformylase, equivalent to CAC30884.1|AL583923 polypeptide deformylase from Mycobacterium leprae (197 aa). Also similar to others e.g. DEF_ECOLI|P27251|95874|S23107 polypeptide deformylase from Escherichia coli (169 aa), FASTA scores: opt: 179, E(): 1.8e-05, (34.6% identity in 162 aa overlap); etc. Belongs to the polypeptide deformylase family. Cofactor: binds 1 zinc ion. P9WIJ3 No information pathways 3E3U P96275 P9WIJ3 GO:0042586,GO:0006412,GO:0005506 3.5.1.88 P9WIJ3 NC_000962.3 Mycobrowser_v5 CDS 518733 519041 . + 0 Rv0430 Rv0430 Function unknown Conserved hypothetical protein Rv0430, (MTCY22G10.27), len: 102 aa. Conserved hypothetical protein, equivalent to AC30882.1|AL583923 conserved hypothetical protein from Mycobacterium leprae (102 aa). Also highly similar to CAB93047.1|SCD95A.20|AL357432 hypothetical protein from Streptomyces coelicolor (84 aa). P96276 No conserved hypotheticals P96276 P96276 NC_000962.3 Mycobrowser_v5 CDS 519073 519567 . + 0 Rv0431 Rv0431 Unknown Putative tuberculin related peptide Rv0431, (MTCY22G10.28), len: 164 aa. Putative tuberculin related peptide; almost identical to D00815|MSGAT103_1 AT103 from Mycobacterium tuberculosis (172 aa), FASTA score: (99.4% identity in 163 aa overlap). Highly similar to to CAC30881.1|AL583923 tuberculin related peptide (AT103) from Mycobacterium leprae (167 aa). Some similarity to G550415|HRPC (282 aa), FASTA scores: opt: 120, E(): 0.36, (33.3% identity in 111 aa overlap). Potential transmembrane domain at aa 19-37. P96277 No cell wall and cell processes P96277 P96277 NC_000962.3 Mycobrowser_v5 CDS 519600 520322 . + 0 Rv0432 sodC Destroys radicals which are normally produced within the cells and are toxic to biological systems [catalytic activity: 2 superoxide + 2 H+ = O2 + H2O2]. Periplasmic superoxide dismutase [Cu-Zn] SodC Rv0432, (MTCY22G10.29), len: 240 aa. sodC, periplasmic superoxide dismutase [Cu-Zn], equivalent to CAC30880.1|AL583923 superoxide dismutase precursor (Cu-Zn) from Mycobacterium leprae (240 aa); and AAK20038.1|AF326234_1 copper zinc superoxide dismutase from Mycobacterium avium subsp. paratuberculosis (226 aa). Also similar to others e.g. SODC_PHOLE|P00446 superoxide dismutase precursor (Cu-Zn) from Photobacterium leiognathi (173 aa), FASTA scores: opt: 214, E(): 5.2 e-06, (36.5% identity in 181 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Belongs to the Cu-Zn superoxide dismutase family. Possibly localized in periplasm, membrane-bound. P9WGE9 No virulence, detoxification, adaptation 1PZS P0A608 P9WGE9 GO:0055114,GO:0005886,GO:0004784,GO:0006801,GO:0046872 1.15.1.1 P9WGE9 NC_000962.3 Mycobrowser_v5 CDS 1901047 1901751 . + 0 Rv1676 Rv1676 Unknown Unknown protein Rv1676, (MTV047.11), len: 234 aa. Unknown protein. O53923 No conserved hypotheticals O53923 O53923 GO:0016491,GO:0016209 O53923 NC_000962.3 Mycobrowser_v5 CDS 552614 554008 . + 0 Rv0462 lpdC Involved in energy metabolism. Lipoamide dehydrogenase is the E3 component of pyruvate dehydrogenase and the alpha-ketoacid dehydrogenase complex [catalytic activity: dihydrolipoamide + NAD(+) = lipoamide + NADH]. Also involved in antioxidant defense; LPDC|Rv0462, DLAT|Rv2215, AHPD|Rv2429, and AHPC|Rv2428 constitute an NADH-dependent peroxidase and peroxynitrite reductase that provides protection against oxidative stress. Dihydrolipoamide dehydrogenase LpdC (lipoamide reductase (NADH)) (lipoyl dehydrogenase) (dihydrolipoyl dehydrogenase) (diaphorase) Rv0462, (MTV038.06), len: 464 aa. LpdC (alternate gene name: TB49.2, CIP50), dihydrolipoamide dehydrogenase (see Argyrou & Blanchard 2001), equivalent to AAA63016.1|U15183 lipoamide dehydrogenase from Mycobacterium leprae (467 aa), FASTA scores: opt: 2583, E(): 0, (83.1% identity in 467 aa overlap). Also similar to to many e.g. P50970|DLDH_ZYMMO|X82291|ZMLPD_1 dihydrolipoamide dehydrogenase from Z.mobilis (466 aa), FASTA scores: opt: 1198, E(): 0, (42.4 % identity in 465 aa overlap); etc. Belongs to the pyridine nucleotide-disulfide oxidoreductases class-I. Binds to coronin-1 in BCG and M. tuberculosis - coronin-1 is retained on phagosomes and phagosome maturation is arrested (See Deghmane et al., 2007). LpdC|Rv0462 co-immunoprecipitates with DlaT|Rv2215 (in lpdC|Rv0462 mutant) and with BkdC|Rv2495c (in dlaT|Rv2215 mutant) (See Venugopal et al., 2011). P9WHH9 No intermediary metabolism and respiration 2A8X,3II4 P66004 P9WHH9 GO:0016209,GO:0045454,GO:0005737,GO:0004148,GO:0006096,GO:0055114,GO:0050660 1.8.1.4 P9WHH9 NC_000962.3 Mycobrowser_v5 CDS 524530 525390 . - 0 Rv0436c pssA Involved in phospholipid biosynthesis. Generates phosphatidylserine [catalytic activity: CDP-diacylglycerol + L-serine = CMP + O-SN-phosphatidyl-L-serine]. Probable CDP-diacylglycerol--serine O-phosphatidyltransferase PssA (PS synthase) (phosphatidylserine synthase) Rv0436c, (MTCY22G10.33c), len: 286 aa. Probable pssA, PS synthase (CDP-diacylglycerol--serine O-phosphatidyltransferase) (see citation below), integral membrane protein, equivalent to AL035159|MLCB1450_9|T44730 from Mycobacterium leprae (300 aa), FASTA scores: opt: 1506, E(): 0, (77.9% identity in 285 aa overlap). Also highly similar to others e.g. NP_108059.1|14027250|BAB54204.1|AP003012 phosphatidylserine synthase from Mesorhizobium loti (248 aa); PSS_BACSU|P39823 cdp-diacylglycerol--serine o-phosphatidyltransferase from Bacillus subtilis (177 aa), FASTA scores: opt: 277, E(): 9.9e-12, (33.3% identity in 183 aa overlap); etc. Contains PS00379 CDP-alcohol phosphatidyltransferases signature. Belongs to the CDP-alcohol phosphatidyltransferase class-I family. P9WPG1 No lipid metabolism P96282 P9WPG1 GO:0003882,GO:0016021,GO:0008654,GO:0005886,GO:0046341 2.7.8.8 NC_000962.3 Mycobrowser_v5 CDS 526143 527360 . - 0 Rv0438c moeA2 Involved in molybdenum cofactor biosynthesis: involved in the biosynthesis of a demolybdo-cofactor (molybdopterin), necessary for molybdo-enzymes. Probable molybdopterin biosynthesis protein MoeA2 Rv0438c, (MTV037.02c), len: 405 aa. Probable moeA2, molybdenum cofactor biosynthesis protein, highly similar to many e.g. Y10817|ANY10817_2 from A. nicotinovorans (429 aa), FASTA scores: opt: 786, E(): 0, (39.2% identity in 398 aa overlap); etc. Also similar to MOEA1|Rv0994|MTCI237.08|O05577 probable molybdopterin biosynthesis protein from Mycobacterium tuberculosis (426 aa), FASTA scores: opt: 667, E(): 2e-32, (36.5% identity in 425 aa overlap). Note that previously known as moeA3. P9WJQ5 No intermediary metabolism and respiration O53725 P9WJQ5 GO:0046872,GO:0016740,GO:0006777 2.-.-.- P9WJQ5 NC_000962.3 Mycobrowser_v5 CDS 554313 554885 . - 0 Rv0464c Rv0464c Function unknown Conserved protein Rv0464c, (MTV038.08c), len: 190 aa. Conserved protein, highly similar to CAC31982.1|AL583925 conserved hypothetical protein from Mycobacterium leprae (188 aa). Also some similarity with Rv1531|AL022000|MTV045_5|D70820 hypothetical protein from Mycobacterium tuberculosis (188 aa), FASTA scores: E(): 9.6e-10, (30.9% identity in 175 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53749 No conserved hypotheticals O53749 O53749 O53749 NC_000962.3 Mycobrowser_v5 CDS 3964479 3965192 . - 0 Rv3528c Rv3528c Unknown Unknown protein Rv3528c, (MTCY03C7.28), len: 237 aa. Unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6YGE4 No conserved hypotheticals I6YGE4 I6YGE4 NC_000962.3 Mycobrowser_v5 CDS 530296 530724 . - 0 Rv0441c Rv0441c Unknown Hypothetical protein Rv0441c, (MTV037.05c), len: 142 aa. Hypothetical unknown protein. P9WKW3 No conserved hypotheticals P9WKW3 P9WKW3 NC_000962.3 Mycobrowser_v5 CDS 530751 532214 . - 0 Rv0442c PPE10 Function unknown PPE family protein PPE10 Rv0442c, (MTV037.06c), len: 487 aa. PPE10, Member of the Mycobacterium tuberculosis PPE family, nearly identical to hypothetical protein from Mycobacterium tuberculosis (strain Erdman) and to AN5S46909_1 protein fragment from Mycobacterium bovis (302 aa); P42611|YHS6_MYCTU hypothetical 50.6 kDa protein (517 aa), FASTA scores: opt: 3144, E(): 0, (98.4 identity in 492 aa overlap); and S46909|S46909_1 (302 aa), FASTA scores: opt: 1897, E(): 0, (98.0% identity in 302 aa overlap). Nucleotide position 532097 in the genome sequence has been corrected, T:C resulting in K40E. P9WI41 No PE/PPE P42611 P9WI41 P9WI41 NC_000962.3 Mycobrowser_v5 CDS 547076 547357 . - 0 Rv0456A mazF1 Function unknown Possible toxin MazF1 Rv0456A, len: 93 aa. Possible mazF1, toxin, part of toxin-antitoxin (TA) operon with Rv0456B (See Pandey and Gerdes, 2005; Zhu et al., 2006); N-terminus highly similar to N-terminal part of P71650|Rv2801c|MT2869|MTCY16B7.42 conserved hypothetical protein from Mycobacterium tuberculosis (118 aa), FASTA scores: opt: 303, E(): 1e-14, (60.44% identity in 91 aa overlap). Also some similarity in part with other hypothetical proteins e.g. Q9PHH8|XFA0027 Plasmid maintenance protein from Xylella fastidiosa (108 aa), FASTA scores: opt: 169, E(): 3.9e-05, (50.820% identity in 61 aa overlap). Q6MX40 No virulence, detoxification, adaptation Q6MX40 Q6MX40 GO:0003677 Q6MX40 NC_000962.3 Mycobrowser_v5 CDS 533091 533789 . - 0 Rv0444c rskA Regulates negatively Rv0445c|SIGK Anti-sigma factor RskA (regulator of sigma K) Rv0444c, (MTV037.08c), len: 232 aa. RskA, regulator of SigK (See Said-Salim et al., 2006); C-terminus similar to P12752|Y24K_STRGR hypothetical 24.7 kDa protein from Streptomyces griseus (238 aa), FASTA scores: opt: 207, E(): 2.2e-05, (32.9% identity in 158 aa overlap). Cleaved by Rip|Rv2869c, in M. tuberculosis Erdman (See Sklar et al., 2010). P9WGX5 No information pathways O53729 P9WGX5 GO:0005886,GO:0016021 P9WGX5 NC_000962.3 Mycobrowser_v5 CDS 1917756 1917932 . + 0 Rv1693 Rv1693 Unknown Conserved hypothetical protein Rv1693, (MTCI125.15), len: 58 aa. Conserved hypothetical protein, shows some similarity to AL583921 hypothetical protein from Mycobacterium leprae (61 aa). Probable coiled-coil from aa 30 to 58. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O33195 No conserved hypotheticals O33195 O33195 NC_000962.3 Mycobrowser_v5 CDS 534445 535215 . - 0 Rv0446c Rv0446c Unknown Possible conserved transmembrane protein Rv0446c, (MTV037.10c), len: 256 aa. Possible conserved transmembrane protein, similar at N-terminus to U1740AF|U15183|MLU15183_40 from Mycobacterium leprae (117 aa), FASTA scores: opt: 175, E(): 2.5e-05, (62.5% identity in 40 aa overlap); and at C-terminus to AL021529|SC10A5_3 from Streptomyces coelicolor (226 aa), FASTA scores: opt: 207, E(): 9.8e-07, (34.2% identity in 114 aa overlap). Also similar to others hypothetical proteins e.g. AAK04680.1|AE006291_14|AE006291 hypothetical protein from Lactococcus lactis subsp. lactis (257 aa). O53731 No cell wall and cell processes O53731 O53731 GO:0016021,GO:0006629,GO:0016627,GO:0005737 O53731 NC_000962.3 Mycobrowser_v5 CDS 536504 537169 . - 0 Rv0448c Rv0448c Function unknown Conserved hypothetical protein Rv0448c, (MTV037.12c), len: 221 aa. Conserved hypothetical protein, similar to other hypothetical proteins e.g. Z74841|BOD5A2_1 from B. oleracea (283 aa), FASTA scores: opt: 257, E(): 1.4e-10, (32.0% identity in 197 aa overlap); etc. Some similarity to U15183|MLU15183_38 from Mycobacterium leprae (82 aa), FASTA scores: opt: 134, E(): 0.014, (71.0% identity in 31 aa overlap). O53733 No conserved hypotheticals O53733 O53733 NC_000962.3 Mycobrowser_v5 CDS 537229 538548 . - 0 Rv0449c Rv0449c Function unknown Conserved hypothetical protein Rv0449c, (MTV037.13c), len: 439 aa. Conserved hypothetical protein, some similarity with several hypothetical proteins and various enzymes e.g. AAK24569.1|AE005927 amine oxidase, flavin-containing from Caulobacter crescentus (454 aa); BAB02771.1|AB023036 mycolic acid methyl transferase-like protein from Arabidopsis thaliana (842 aa); BAB01742.1|AP000374 protein which contains similarity to cyclopropane fatty acid synthase from Arabidopsis thaliana (793 aa); etc. Has hydrophobic stretch at N-terminus. O53734 No conserved hypotheticals O53734 O53734 O53734 NC_000962.3 Mycobrowser_v5 CDS 543174 544730 . + 0 Rv0453 PPE11 Function unknown PPE family protein PPE11 Rv0453, (MTV037.17), len: 518 aa. PPE11, Member of the Mycobacterium tuberculosis PPE family, similar to many e.g. AL0212|MTV012_32 from Mycobacterium tuberculosis (434 aa), FASTA scores: opt: 882, E(): 7e-31, (41.8% identity in 514 aa overlap). P9WI39 No PE/PPE Q6MX41 P9WI39 P9WI39 NC_000962.3 Mycobrowser_v5 CDS 632372 633034 . + 0 Rv0540 Rv0540 Function unknown Conserved hypothetical protein Rv0540, (MTCY25D10.19), len: 220 aa. Conserved hypothetical protein, similar to hypothetical proteins from Streptomyces coelicolor: CAB76990.1|AL159178 (213 aa); N-terminus of BAA84086.1|AB032065 (446 aa); and CAB61872.1|AL133252|SCE46_21 (210 aa), FASTA scores: opt: 267, E(): 5.3e-10, (32.7% identity in 202 aa overlap). Also some similarity with D90913_63|PCC6803 from Synecho cystis sp (211 aa), FASTA scores: opt: 189, E(): 4.7e-06, (25.3 identity in 194 aa overlap). O06406 No conserved hypotheticals O06406 O06406 O06406 NC_000962.3 Mycobrowser_v5 CDS 541488 541910 . - 0 Rv0451c mmpS4 Unknown Probable conserved membrane protein MmpS4 Rv0451c, (MTV037.15c), len: 140 aa. Probable mmpS4, conserved membrane protein (see citations below), equivalent to U1740W|P54880|YV33_MYCLE hypothetical 16.9 kDa protein from Mycobacterium leprae (154 aa), FASTA scores: opt: 727, E(): 0, (75.9% identity in 137 aa overlap). Also similar to other Mycobacterial proteins e.g. Z84725|MTCY04D9.16c from Mycobacterium tuberculosis (142 aa), FASTA scores: opt: 451, E(): 3.2e-24, (50.0% identity in 138 aa overlap); etc. Belongs to the MmpS family. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). P9WJS9 No cell wall and cell processes P0A5K2 P9WJS9 GO:0016021 P9WJS9 NC_000962.3 Mycobrowser_v5 CDS 542142 542852 . + 0 Rv0452 Rv0452 Possibly involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0452, (MTV037.16), len: 236 aa. Possible transcriptional regulator, similar to several putative TetR-family transcriptional regulators from Streptomyces coelicolor. Also similar in N-terminus to U1740Y|U15183|MLU15183_33 from Mycobacterium leprae (67 aa), FASTA score: (76.1% identity in 67 aa overlap). Contains probable helix-turn-helix motif at aa 44-65 (Score 1727, +5.07 SD). O53737 No regulatory proteins O53737 O53737 GO:0006350,GO:0003700,GO:0006355 O53737 NC_000962.3 Mycobrowser_v5 CDS 547586 549607 . - 0 Rv0457c Rv0457c Function unknown; hydrolyzes peptides and/or proteins. Probable peptidase Rv0457c, (MTCI429A.01, MTV038.01c), len: 673 aa. Probable peptidase, similar to many e.g. NP_102851.1|14022026|BAB48637.1 probable endopeptidase from Mesorhizobium loti (687 aa); Y4NA_RHISN|P55577 probable peptidase (726 aa), FASTA scores: opt: 1126, E(): 0, (40.9% identity in 491 aa overlap). Also similar to Mycobacterium tuberculosis protein MTCY369.26 FASTA score: (33.8% identity in 299 aa overlap). O07178 No intermediary metabolism and respiration O07178 O07178 GO:0004252,GO:0006508 3.4.-.- O07178 NC_000962.3 Mycobrowser_v5 CDS 549675 551198 . + 0 Rv0458 Rv0458 Interconversion aldehyde and acid [catalytic activity: an aldehyde + NAD+ + H2O = an acid + NADH]. Probable aldehyde dehydrogenase Rv0458, (MTV038.02), len: 507 aa. Probable aldehyde dehydrogenase, highly similar to many, closest to P46369|THCA_RHOER EPTC-inducible aldehyde dehydrogenase from Rhodococcus erythropolis (506 aa), FASTA scores: opt: 2767, E(): 0, (79.7% identity in 507 aa overlap); AAC13641.1|AF029733 chloroacetaldehyde dehydrogenase from Xanthobacter autotrophicus (505 aa), FASTA scores: opt: 2563, E(): 0, (75.4% identity in 492 aa overlap); Q9RJZ6|DHAL_STRCO probable aldehyde dehydrogenase from Streptomyces coelicolor (507 aa). Also similar to other semialdehyde dehydrogenases in Mycobacterium tuberculosis e.g. Rv0768, Rv2858c. Belongs to the aldehyde dehydrogenases family. P9WNY1 No intermediary metabolism and respiration P63937 P9WNY1 GO:0055114,GO:0004029 1.2.1.3 P9WNY1 NC_000962.3 Mycobrowser_v5 CDS 551198 551689 . + 0 Rv0459 Rv0459 Function unknown Conserved hypothetical protein Rv0459, (MTV038.03), len: 163 aa. Conserved hypothetical protein, highly similar to other hypothetical proteins. Note that highly similar to products of unidentified ORFs in Xanthobacter autotrophicus, AF029733_2 (139 aa), and Rhodococcus erythropolis, REREUTP BC_1 (186 aa). Like MTV038.03, these ORF's are linked to aldehyde dehydrogenase genes. FASTA scores: AF0297|AF029733_2 (139 aa), opt: 439, E(): 6.2e-24, (50.0% identity in 126 aa overlap); and L24492|REREUTPBC_1 (186 aa), opt: 347, E(): 2.1e-17, (52.7% identity in 169 aa overlap). N-terminus also highly similar to AAA63041.1|U15183 ethanolamine permease (eutP) match from Mycobacterium leprae (53 aa). O53744 No conserved hypotheticals O53744 O53744 NC_000962.3 Mycobrowser_v5 CDS 551749 551988 . + 0 Rv0460 Rv0460 Function unknown Conserved hydrophobic protein Rv0460, (MTV038.04), len: 79 aa. Conserved hydrophobic protein, highly similar AAA63024.1|U15183 hypothetical protein from Mycobacterium leprae (56 aa), FASTA scores: opt: 197, E(): 3.7e-09, (63.8% identity in 47 aa overlap). O53745 No conserved hypotheticals O53745 NC_000962.3 Mycobrowser_v5 CDS 556458 557252 . + 0 Rv0466 Rv0466 Function unknown Conserved protein Rv0466, (MTV038.10), len: 264 aa. Conserved protein, equivalent to CAC31980.1|AL583925 conserved hypothetical protein from Mycobacterium leprae (264 aa). Similar to Rv2001|Z74025|MTCY39.17c hypothetical 28.7 KDA protein from Mycobacterium tuberculosis (250 aa), FASTA scores: opt: 592, E(): 0, (38.0% identity in 263 aa overlap). Some similarity to several thioesterases e.g. Q42561|ATACPTE17_1 acyl-(acyl carrier protein) thioester from A. thaliana (362 aa), FASTA scores: E(): 0.0092, (24.4% identity in 197 aa overlap). O53751 No conserved hypotheticals O53751 O53751 O53751 NC_000962.3 Mycobrowser_v5 CDS 558895 559755 . + 0 Rv0468 fadB2 Butyrate/butanol-producing pathway [catalytic activity: (S)-3-hydroxybutanoyl-CoA + NADP+ = 3-acetoacetyl-CoA + NADPH] 3-hydroxybutyryl-CoA dehydrogenase FadB2 (beta-hydroxybutyryl-CoA dehydrogenase) (BHBD) Rv0468, (MTV038.12), len: 286 aa. fadB2, 3-hydroxybutyryl-CoA dehydrogenase, equivalent to CAC31978.1|AL583925 3-hydroxyacyl-CoA dehydrogenase from Mycobacterium leprae (287 aa). Also similar to many 3-hydroxybutyryl-CoA dehydrogenases e.g. U32229|BJU32229_1 beta-hydroxybutyryl coenzyme A dehydrogenase from Bradyrhizobium japonicum (293 aa), FASTA scores: opt: 771, E(): 0, (45.7% identity in 282 aa overlap). Belongs to the 3-hydroxyacyl-CoA dehydrogenase family. P9WNP7 No lipid metabolism O53753 P9WNP7 GO:0050662,GO:0006631,GO:0055114,GO:0008691 1.1.1.157 P9WNP7 NC_000962.3 Mycobrowser_v5 CDS 559888 560748 . + 0 Rv0469 umaA Involved in mycolic acid modification or synthesis. Possible mycolic acid synthase UmaA Rv0469, (MTV038.13), len: 286 aa. Possible umaA, mycolic acid synthase (see citations below), highly similar to CAC30854.1|AL583923 methyl mycolic acid synthase 1 from Mycobacterium leprae (286 aa); and CAC31976.1|AL583925 Mycolic acid synthase from Mycobacterium leprae (295 aa), FASTA scores: opt: 1402, E(): 0, (69.6% identity in 286 aa overlap). Also very similar to mycobacterial methyltransferases e.g. U77466|CmaD|MBU77466_1 (286 aa); MTCY20H10.26c|Z92772|MTY20H10_27 (296 aa); highly similar to CFA1_MYCTU|Q11195|U66108|MTU66108_1 cyclopropane-fatty-acyl-phospholipid synthase 1 (287 aa), FASTA scores: opt: 1360, E(): 0, (67.8% identity in 286 aa overlap) (see citation below); and very similar also to methoxy mycolic acid synthase 1 from Mycobacterium tuberculosis e.g. MTU66108_1 (286 aa). Note that previously known as umaA1. Q6MX39 No lipid metabolism Q6MX39 Q6MX39 GO:0008610,GO:0008825 2.-.-.- Q6MX39 NC_000962.3 Mycobrowser_v5 CDS 566508 566771 . + 0 Rv0476 Rv0476 Unknown Possible conserved transmembrane protein Rv0476, (MTCY20G9.02), len: 87 aa. Possible conserved transmembrane protein, equivalent to CAC31970.1|AL583925 conserved membrane protein from Mycobacterium leprae (95 aa). Also highly similar to CAC04036.1|AL391406 putative membrane protein from Streptomyces coelicolor (113 aa). Contains PS00606 Beta-ketoacyl synthases active site. P9WKW1 No cell wall and cell processes P64695 P9WKW1 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1108172 1108504 . - 0 Rv0991c Rv0991c Function unknown Conserved serine rich protein Rv0991c, (MTCI237.05c), len: 110 aa. Conserved ser-rich protein (especially in C-terminus), highly similar to N-terminus of NP_301255.1|NC_002677 conserved hypothetical protein (Ser-rich C-terminus) from Mycobacterium leprae (99 aa). Also highly similar to SCE22.04|AB90971.1|AL355832 hypothetical protein from Streptomyces coelicolor (110 aa); and similar to others. O05574 No conserved hypotheticals O05574 O05574 NC_000962.3 Mycobrowser_v5 CDS 561854 562294 . - 0 Rv0470A Rv0470A Unknown Hypothetical protein Rv0470A, len: 146 aa. Hypothetical unknown protein. GC plot suggests CDS for Cys-rich protein, could possibly be continuation of Rv0471c but no frameshift found to allow this. Sequence same in Mycobacterium bovis and Mycobacterium tuberculosis strain CDC1551. Weak hits to Cys-rich region (aa 258-314) of D63395|D63395_1 mRNA for NOTCH4 from Homo sapiens (1095 aa), FASTA scores: opt: 132, E(): 1.1, (39.35% identity in 61 aa overlap). L7N651 No conserved hypotheticals Q8VKK9 L7N651 GO:0004659,GO:0016021 L7N651 NC_000962.3 Mycobrowser_v5 CDS 562225 562713 . - 0 Rv0471c Rv0471c Unknown Hypothetical protein Rv0471c, (MTV038.15c), len: 162 aa. Hypothetical unknown protein. O53756 No conserved hypotheticals O53756 NC_000962.3 Mycobrowser_v5 CDS 563564 564934 . + 0 Rv0473 Rv0473 Unknown Possible conserved transmembrane protein Rv0473, (MTV038.17), len: 456 aa. Possible conserved transmembrane protein, showing some similarity to hypothetical proteins e.g. NP_102800.1|14021975|BAB48586.1|AP002996 hypothetical protein from Mesorhizobium loti (431 aa); P39385|YJIN_ECOLI|YJIN|B4336 hypothetical 48.2 kDa protein (potential integral membrane protein) from Escherichia coli strain K12 (426 aa), FASTA scores: opt: 396, E(): 9.8e-19, (31.8 % identity in 424 aa overlap); etc. Nucleotide position 563577 in the genome sequence has been corrected, A:G resulting in K5R. I6Y3V3 No cell wall and cell processes O53758 I6Y3V3 GO:0016021 I6Y3V3 NC_000962.3 Mycobrowser_v5 CDS 565021 565443 . + 0 Rv0474 Rv0474 Possibly involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv0474, (MTV038.18), len: 140 aa. Probable transcriptional regulator, highly similar to others e.g. CAC04034.1|AL391406 putative DNA-binding protein from Streptomyces coelicolor (141 aa); N-terminus of NP_104173.1|14023352|BAB49959.1|AP003000 transcriptional regulator from Mesorhizobium loti (219 aa); N-terminus of A83618|PA0225 probable transcription regulator from Pseudomonas aeruginosa (179 aa); SINR_BACSU|P06533 sinr protein from Bacillus subtilis (111 aa), FASTA scores: opt: 147, E(): 8.9e-06, (30.6% identity in 111 aa overlap). Also similar to other hypothetical proteins e.g. X66407|RRPHAS_1|ORF1 from Rhodococcus ruber (171 aa), FASTA scores: opt: 280, E(): 4.8e-12, (43.6% identity in 117 aa overlap). Also similar to Rv2745c from Mycobacterium tuberculosis. Contains probable helix-turn-helix domain at aa 35-56 (Score 1709, +5.01 SD). P9WMH9 No regulatory proteins O53759 P9WMH9 GO:0043565,GO:0006350,GO:0045449 P9WMH9 NC_000962.3 Mycobrowser_v5 CDS 594802 595302 . - 0 Rv0504c Rv0504c Function unknown Conserved protein Rv0504c, (MTCY20G9.31c), len: 166 aa. Conserved protein, equivalent to P54879|Y504_MYCLE|ML2425 hypothetical 18.7 KDA protein from Mycobacterium leprae (166 aa), FASTA scores: opt: 884, E(): 0, (83.1% identity in 166 aa overlap); and highly similar to other proteins from Mycobacterium leprae. Also highly similar to CAB77410.1|AL160431|SCD82.07 hypothetical protein from Streptomyces coelicolor (150 aa). Also similar to M. tuberculosis hypothetical proteins Rv0635|H70612 (158 aa); and Rv0637|B70613 (166 aa). P9WFK3 No conserved hypotheticals P64725 P9WFK3 GO:0016491,GO:0008152 P9WFK3 NC_000962.3 Mycobrowser_v5 CDS 715370 716317 . + 0 Rv0622 Rv0622 Unknown Possible membrane protein Rv0622, (MTCY20H10.03), len: 315 aa. Possible membrane protein; contains potential membrane spanning region. Shows weak similarity with Mycobacterium tuberculosis hypothetical proteins Rv1804c, Rv1810, etc. Start changed since first submission (-26 aa). P96912 No cell wall and cell processes P96912 P96912 NC_000962.3 Mycobrowser_v5 CDS 567222 567896 . + 0 Rv0478 deoC Involved in nucleotide and deoxyribonucleotide catabolism [catalytic activity: 2-deoxy-D-ribose 5-phosphate = D-glyceraldehyde 3-phosphate + acetaldehyde]. Probable deoxyribose-phosphate aldolase DeoC (phosphodeoxyriboaldolase) (deoxyriboaldolase) Rv0478, (MTCY20G9.04), len: 224 aa. Probable deoC, deoxyribose-phosphate aldolase, equivalent to Q9CB45|DEOC_MYCLE deoxyribose-phosphate aldolase from Mycobacterium leprae (226 aa). Also highly similar to others e.g. DEOC_BACSU|P39121 from Bacillus subtilis (214 aa), FASTA scores: opt: 543, E(): 1.4e-26, (45.9% identity in 209 aa overlap); etc. Belongs to the DEOC/FBAB family of aldolases, DEOC subfamily. P9WP03 No intermediary metabolism and respiration P63929 P9WP03 GO:0005737,GO:0009264,GO:0004139,GO:0016052 4.1.2.4 P9WP03 NC_000962.3 Mycobrowser_v5 CDS 567921 568967 . - 0 Rv0479c Rv0479c Unknown Probable conserved membrane protein Rv0479c, (MTCY20G9.04c), len: 348 aa. Probable conserved membrane protein, equivalent to CAC31967.1|AL583925 possible secreted protein from Mycobacterium leprae (254 aa); and C-terminus highly similar to AAF74996.1|AF143402_1|AF143402 putative multicopper oxidase from Mycobacterium avium (149 aa). Contains hydrophobic domain in centre of protein. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WKV7 No cell wall and cell processes P9WKV7 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 569988 570512 . - 0 Rv0481c Rv0481c Unknown Hypothetical protein Rv0481c, (MTCY20G9.07c), len: 174 aa. Hypothetical unknown protein. P9WKV5 No conserved hypotheticals P64701 P9WKV5 P9WKV5 NC_000962.3 Mycobrowser_v5 CDS 586394 587380 . + 0 Rv0496 Rv0496 Function unknown Conserved hypothetical protein Rv0496, (MTCY20G9.22), len: 328 aa. Conserved hypothetical protein, highly similar to S72894|467046|AAA17230.1|U00018 exopolyphosphatase ppx from Mycobacterium leprae (406 aa), FASTA scores: opt: 1902, E(): 0, (86.6% identity in 343 aa overlap); and P54882|Y496_MYCLE|ML2434|13094008|CAC31951.1|AL583925 hypothetical 36.2 KDA protein from Mycobacterium leprae (339 aa). Also highly similar to hypothetical proteins and exopolyphosphatases e.g. Q9X8H1|Y715_STRCO|SCE7.15c hypothetical protein from Streptomyces coelicolor (309 aa). C-terminal region similar to CGU31224_1|Q46054 protein similar to ppx gene product of Mycobacterium leprae from Cornybacterium glutamicum (140 aa), FASTA scores: opt: 615, E(): 2.7e-33, (70.9% identity in 134 aa overlap). P9WHV5 No conserved hypotheticals P65786 P9WHV5 GO:0016787 P9WHV5 NC_000962.3 Mycobrowser_v5 CDS 610188 610883 . + 0 Rv0518 Rv0518 Unknown Possible exported protein Rv0518, (MTCY20G10.08), len: 231 aa. Possible exported protein; has hydrophobic N-terminus. O33363 No cell wall and cell processes O33363 GO:0016787 O33363 NC_000962.3 Mycobrowser_v5 CDS 1504356 1505078 . + 0 Rv1337 Rv1337 Unknown Probable integral membrane protein Rv1337, (MTCY130.22), len: 240 aa. Probable integral membrane protein. Highly similar to P53426 hypothetical protein B1549_C3_240 from M.leprae (251); and P74553|D90916 hypothetical protein from Synechocystis sp. (198 aa), FASTA scores: E(): 2.3e-25, (43.6% identity in 181 aa overlap). P9WM21 No cell wall and cell processes P9WM21 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 4061899 4062198 . - 0 Rv3622c PE32 Function unknown PE family protein PE32 Rv3622c, (MTCY15C10.30), len: 99 aa. PE32, Member of the Mycobacterium tuberculosis PE family (see Brennan and Delogu, 2002), but no glycine rich C-terminus present. Similar to others e.g. O53938|Rv1788|MTV049.10 (99 aa), FASTA scores: opt: 376, E(): 7.1e-17, (65.6% identity in 96 aa overlap). I6YGS7 No PE/PPE Q7D568 I6YGS7 I6YGS7 NC_000962.3 Mycobrowser_v5 ncRNA 575033 575069 . + 0 MTB000060 mcr19 Unknown Fragment of putative small regulatory RNA mcr19, fragment of putative small regulatory RNA (See DiChiara et al., 2010), cloned from M. bovis BCG Pasteur; ends not mapped, 66-82 nt band detected by Northern blot in M. bovis BCG Pasteur. No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 571710 573065 . + 0 Rv0483 lprQ Unknown Probable conserved lipoprotein LprQ Rv0483, (MTCY20G9.09), len: 451 aa. Probable lprQ, conserved lipoprotein, equivalent to CAC31963.1|AL583925|ML2446 possible lipoprotein from Mycobacterium leprae (441 aa); appears longer than ML2446, so start may be further downstream. Shows also similarity with MLCL383_24|O07707 hypothetical 43.6 kDa protein from Mycobacterium leprae; and to Q49706|B1496_F2_81 (271 aa). Similar to others lipoproteins from other organisms. Also similar to several Mycobacterium tuberculosis hypothetical proteins e.g. Rv0116c, Rv0192, Rv1433, Rv2518c. Contains potential N-terminal signal sequence and appropriately positioned PS00013 prokaryotic membrane lipoprotein lipid attachment site. P9WKV3 No cell wall and cell processes P64703 P9WKV3 GO:0016787,GO:0016021,GO:0009252,GO:0008360,GO:0016757,GO:0007047 2.-.-.- P9WKV3 NC_000962.3 Mycobrowser_v5 CDS 573046 573801 . - 0 Rv0484c Rv0484c Function unknown; possibly involved in cellular metabolism. Probable short-chain type oxidoreductase Rv0484c, (MTCY20G9.10c), len: 251 aa. Probable short-chain oxidoreductase, highly similar to others e.g. T36118|4678912|CAB41284.1|AL049707 probable oxidoreductase from Streptomyces coelicolor (260 aa); YDFG_HAEIN|P45200|HI1430 hypothetical oxidoreductase (SDR family) from Haemophilus influenzae (252 aa), FASTA scores: opt: 496, E(): 7.9e-25, (35.0 % identity in 243 aa overlap); etc. Belongs to the short-chain dehydrogenases/reductases (SDR) family. Strong similarity, to bacterial YDFG homologs. P9WGR5 No intermediary metabolism and respiration Q11150 P9WGR5 GO:0055114,GO:0016491 1.-.-.- P9WGR5 NC_000962.3 Mycobrowser_v5 CDS 573984 575300 . + 0 Rv0485 Rv0485 Possibly involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0485, (MTCY20G9.11), len: 438 aa. Possible transcriptional repressor, member of the NAGC/XYLR repressor family; similar to several e.g. D87820_3|O32446|D82254 NAGC N-acetylglucosamine repressor from Vibrio cholerae (404 aa), FASTA scores: opt: 378, E(): 1.2e-17, (26.9% identity in 350 aa overlap); NAGC_ECOLI|P15301 N-acetylglucosamine repressor from Escherichia coli (406 aa), FASTA scores: opt: 305, E(): 1.8e-12, (21.8% identity in 357 aa overlap); etc. P9WKV1 No regulatory proteins P64705 P9WKV1 P9WKV1 NC_000962.3 Mycobrowser_v5 CDS 585424 586314 . - 0 Rv0495c Rv0495c Function unknown Conserved hypothetical protein Rv0495c, (MTCY20G9.21c), len: 296 aa. Conserved hypothetical protein, highly similar to S72915|B2168_F1_37 hypothetical protein from Mycobacterium leprae (323 aa), FASTA scores: opt: 1615, E(): 0, (82.7% identity in 271 aa overlap); and P54579|Y495_MYCLE|ML243|13094009|CAC31952.1|AL583925 conserved hypothetical protein from Mycobacterium leprae (277 aa). Also highly similar to Q9X8H2|Y716_STRCO|SCE7.16 hypothetical protein from Streptomyces coelicolor (271 aa). P9WKU5 No conserved hypotheticals P9WKU5 NC_000962.3 Mycobrowser_v5 CDS 587377 588309 . + 0 Rv0497 Rv0497 Unknown Probable conserved transmembrane protein Rv0497, (MTCY20G9.23), len: 310 aa. Probable conserved transmembrane protein, equivalent (but shorter in C-terminus) to P54580|Y497_MYCLE|ML2433 hypothetical 37.9 KDA protein from Mycobacterium leprae (355 aa). N-terminus highly similar to S72922|B2168_C1_166|467074 hypothetical protein from Mycobacterium leprae (118 aa), FASTA scores: opt: 350, E(): 1.4e-12, (57.9% identity in 114 aa overlap); and hydrophobic C-terminus, highly similar to S72895|B2168_C2_209|467047 hypothetical protein from Mycobacterium leprae (241 aa), FASTA scores: opt: 473, E(): 8e-19, (53.9% identity in 241 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WKU3 No cell wall and cell processes P9WKU3 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 933003 934031 . - 0 Rv0837c Rv0837c Unknown Hypothetical protein Rv0837c, (MTV043.30c), len: 342 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6Y4Y1 No conserved hypotheticals O53849 I6Y4Y1 I6Y4Y1 NC_000962.3 Mycobrowser_v5 CDS 576787 577338 . + 0 Rv0487 Rv0487 Function unknown Conserved hypothetical protein Rv0487, (MTCY20G9.13), len: 183 aa. Conserved hypothetical protein, highly similar to P54139|Y487_MYCLE|U00018_38|ML2442 hypothetical 20.8 KDA protein from Mycobacterium leprae (184 aa), FASTA scores: opt: 760, E(): 2.4 e-34, (73.0% identity in 159 aa overlap). Also highly similar to CAC04041.1|AL391406 conserved hypothetical protein from Streptomyces coelicolor (168 aa). P9WKU9 No conserved hypotheticals P64709 P9WKU9 P9WKU9 NC_000962.3 Mycobrowser_v5 CDS 578426 579175 . + 0 Rv0489 gpm1 Involved in glycolysis [catalytic activity: 1,3-diphosphoglycerate + 3-phosphoglycerate = 2,3-diphosphoglycerate + 3-phosphoglycerate]. Probable phosphoglycerate mutase 1 Gpm1 (phosphoglyceromutase) (PGAM) (BPG-dependent PGAM) Rv0489, (MTCY20G9.15), len: 249 aa. Probable gpm1, phosphoglycerate mutase 1, equivalent to P53531|PMGY_MYCLE phosphoglycerate mutase from Mycobacterium leprae (247 aa). Also highly similar to others e.g. PMG1_ECOLI|P31217 (249 aa), FASTA scores: opt: 805, E(): 0, (51.4% identity in 245 aa overlap); etc. Contains PS00175 Phosphoglycerate mutase family phosphohistidine signature, and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the phosphoglycerate mutase family. Note that previously known as gpm. P9WIC9 No intermediary metabolism and respiration 1RII P0A5R6 P9WIC9 GO:0006096,GO:0046538 5.4.2.1 P9WIC9 NC_000962.3 Mycobrowser_v5 CDS 588325 589167 . + 0 Rv0498 Rv0498 Function unknown Conserved hypothetical protein Rv0498, (MTCY20G9.24), len: 280 aa. Conserved hypothetical protein, highly similar to P54581|Y498_MYCLE|ML2432 hypothetical 30.5 KDA protein from Mycobacterium leprae (280 aa); and S72896|B2168_C2_210 hypothetical protein from Mycobacterium leprae (244 aa), FASTA scores: opt: 1486, E():0, (89.3% identity in 244 aa overlap). Also similar to Q9X8H0|Y714_STRCO|SCE7.14c hypothetical protein from Streptomyces coelicolor. P9WKU1 No conserved hypotheticals P64717 P9WKU1 P9WKU1 NC_000962.3 Mycobrowser_v5 CDS 1485313 1485771 . - 0 Rv1322A Rv1322A Function unknown Conserved protein Rv1322A, len: 152 aa. Conserved protein, similar to proteins from Mycobacterium leprae and Streptomyces coelicolor e.g. AL583921_2|ML1157 from M. leprae strain tn (155 aa), FASTA scores: opt: 771, E(): 5.1e-43, (75.3% identity in 154 aa overlap); and AL137242_2 from Streptomyces coelicolor (146 aa), FASTA scores: opt: 404, E(): 2e-19, (43.165% identity in 139 aa overlap). L7N6B1 No conserved hypotheticals Q8VK36 L7N6B1 GO:0016702 L7N6B1 NC_000962.3 Mycobrowser_v5 CDS 1791334 1791573 . + 0 Rv1590 Rv1590 Function unknown Conserved hypothetical protein Rv1590, (MTCY336.14c), len: 79 aa. Conserved hypothetical protein, similar to Q49616|LEPB1170_C1_162|YF90_MYCLE from Mycobacterium leprae (80 aa), FASTA scores: opt: 368, E(): 1.7e-21, Smith-Waterman score: 368, (67.1% identity in 73 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WLT7 No conserved hypotheticals P9WLT7 NC_000962.3 Mycobrowser_v5 CDS 2261098 2261688 . + 0 Rv2014 Rv2014 Required for the transposition of an insertion element. Transposase Rv2014, (MTCY39.03c), len: 196 aa. Transposase, similar to insertion elements; possibly made by frameshifting with respect to Rv2013. Length changed since first submission. Q10843 No insertion seqs and phages Q10843 Q10843 GO:0004803,GO:0006313,GO:0003677 NC_000962.3 Mycobrowser_v5 CDS 2544698 2545225 . + 0 Rv2270 lppN Unknown Probable lipoprotein LppN Rv2270, (MTCY339.40c), len: 175 aa. Probable lppN, lipoprotein; has appropriately positioned prokaryotic membrane lipoprotein attachment site PS00013. P9WK73 No cell wall and cell processes P9WK73 GO:0005886 NC_000962.3 Mycobrowser_v5 CDS 583701 584690 . - 0 Rv0493c Rv0493c Function unknown Conserved protein Rv0493c, (MTCY20G9.19), len: 329 aa. Conserved protein, showing some similarity to U00018_33|B2168_F2_93 from Mycobacterium leprae (167 aa), FASTA scores: opt: 166, E(): 0.00077, (35.9% identity in 131 aa overlap). P9WKU7 No conserved hypotheticals P9WKU7 NC_000962.3 Mycobrowser_v5 CDS 584695 585423 . + 0 Rv0494 Rv0494 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably GntR-family) Rv0494, (MTCY20G9.20), len: 242 aa. Probable transcriptional regulator, GntR family, with C-terminal part highly similar to S72893|B2168_C2_205 hypothetical protein from Mycobacterium leprae (105 aa). Also similar to other transcription regulators e.g. PDHR_ECOLI|P06957 pyruvate dehydrogenase complex repressor PDHR or GENA from Escherichia coli (254 aa), FASTA scores: opt: 284, E(): 1.2e-11, (32.6% identity in 224 aa overlap); etc. Contains PS00043 Bacterial regulatory proteins, gntR family signature, and probable helix-turn helix motif from aa 50-71 (Score 1229, +3.37 SD). P9WMG7 No regulatory proteins P67739 P9WMG7 GO:0006355,GO:0006350,GO:0003700,GO:0005622 P9WMG7 NC_000962.3 Mycobrowser_v5 CDS 606551 608062 . + 0 Rv0515 Rv0515 Function unknown Conserved 13E12 repeat family protein Rv0515, (MTCY20G10.05), len: 503 aa. Part of M. tuberculosis 13E12 repeat family. Almost identical to Rv0336 (99.8% identity in 503 aa overlap), possibly due to a recent gene duplication. Also similar to other M. tuberculosis hypothetical 13E12 repeat proteins e.g. Rv1148c, Rv1945, etc. O33360 No insertion seqs and phages O33360 O33360 GO:0003676,GO:0004519 NC_000962.3 Mycobrowser_v5 CDS 592791 593867 . + 0 Rv0502 Rv0502 Function unknown Conserved protein Rv0502, (MTCY20G9.29), len: 358 aa. Conserved protein, equivalent to P54878|Y502_MYCLE|ML2427 hypothetical 40.5 KDA protein from Mycobacterium leprae (367 aa), FASTA scores: opt: 2042, E(): 0, (84.1% identity in 365 aa overlap). Also similar to T36273|SCE68.23c hypothetical protein from Streptomyces coelicolor (355 aa). C-terminal similar to AL021529|SC10A5_4|T34572 hypothetical protein from Streptomyces coelicolor (295 aa), FASTA score: (57.8% identity in 263 aa overlap); and to hypothetical proteins from Mycobacterium tuberculosis Rv1920|G70808 (287 aa); and Rv1428c|G70914 (275 aa). P9WKT1 No conserved hypotheticals P64723 P9WKT1 GO:0008152,GO:0008415 P9WKT1 NC_000962.3 Mycobrowser_v5 CDS 1733116 1733550 . - 0 Rv1532c Rv1532c Function unknown Conserved hypothetical protein Rv1532c, (MTCY07A7A.01c), len: 144 aa. Conserved hypothetical protein, similar to P20378|YPHR_HALHA Hypothetical 15.6 kDa protein from Halobacterium halobium (151 aa), FASTA scores: opt: 152, E():4.5e-05, (30.1% identity in 103 aa overlap). O06178 No conserved hypotheticals O06178 O06178 O06178 NC_000962.3 Mycobrowser_v5 CDS 4116002 4116379 . + 0 Rv3675 Rv3675 Unknown Possible membrane protein Rv3675, (MTV025.023), len: 125 aa. Possible membrane protein, with some similarity to Q9YCZ2|APE1120 hypothetical 11.7 KDA protein from Aeropyrum pernix (103 aa), FASTA scores: opt: 100, E(): 9, (40.0% identity in 55 aa overlap). I6YCQ7 No cell wall and cell processes I6YCQ7 I6YCQ7 NC_000962.3 Mycobrowser_v5 CDS 590083 590970 . + 0 Rv0500 proC Involved at the terminal (third) step in proline biosynthesis [catalytic activity: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H]. Probable pyrroline-5-carboxylate reductase ProC (P5CR) (P5C reductase) Rv0500, (MTCY20G9.26), len: 295 aa. Probable proC, Pyrroline-5-carboxylate reductase (see citation below), equivalent to P46725|PROC_MYCLE pyrroline-5-carboxylate reductase from Mycobacterium leprae (294 aa), FASTA scores: opt: 1473, E(): 0, (82.4% identity in 295 aa overlap). Also similar to others e.g. P46540|PROC_CORGL pyrroline-5-carboxylate reductase from Corynebacterium glutamicum (270 aa); T36286|4803683|CAB42663.1|AL049819 pyrroline-5-carboxylate reductase from Streptomyces coelicolor (284 aa); etc. Belongs to the pyrroline-5-carboxylate reductase family. P9WHU7 No intermediary metabolism and respiration Q11141 P9WHU7 GO:0005737,GO:0055114,GO:0006561,GO:0004735 1.5.1.2 P9WHU7 NC_000962.3 Mycobrowser_v5 CDS 597199 600105 . + 0 Rv0507 mmpL2 Unknown. Thought to be involved in fatty acid transport. Probable conserved transmembrane transport protein MmpL2 Rv0507, (MTCY20G9.34), len: 968 aa. Probable mmpL2, conserved transmembrane transport protein (see citations below), member of RND superfamily, highly similar to other Mycobacterial proteins e.g. YV34_MYCLE from Mycobacterium leprae (959 aa), FASTA scores: opt: 3699, E(): 0, (58.3% identity in 940 aa overlap); and the Mycobacterium tuberculosis proteins MTV037_14, MTV040_4, MTCY98_8, MTCY4D9_15, MTCY48_8, MTCY19G5_6, MTV005_19, etc. Also similar to STMACTII_3|SC10A5_9 from Streptomyces coelicolor; and BSUB0|004_12 from Bacillus subtilis. C-terminal half similar to Q50086|U1740AB from Mycobacterium leprae (386 aa), FASTA scores: opt: 1526, E(): 0, (61.5% identity in 371 aa overlap). Belongs to the MmpL family. P9WJV7 No cell wall and cell processes Q11171 P9WJV7 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 600441 601847 . + 0 Rv0509 hemA Involved in porphyrin biosynthesis by the C5 pathway (at the first step) [catalytic activity: glutamyl-tRNA(GLU) + NADPH = glutamate-1-semialdehyde + NADP+ + tRNA(GLU)]. Probable glutamyl-tRNA reductase HemA (GLUTR) Rv0509, (MTCY20G9.36), len: 468 aa. Probable hemA, glutamyl-tRNA reductase, equivalent to HEM1_MYCLE|P46724 glutamyl-tRNA reductase from Mycobacterium leprae (467 aa), FASTA scores: opt: 2377, E(): 0, (82.3% identity in 463 aa overlap). Also highly similar (sometimes in part) to others e.g. Q9WX15|HEM1_STRCO glutamyl-tRNA reductase from Streptomyces coelicolor (581 aa); P16618|HEM1_BACSU|HEMA glutamyl-tRNA reductase from Bacillus subtilis (455 aa); etc. Contains PS00747 Glutamyl-tRNA reductase signature. Belongs to the glutamyl-tRNA reductase family. P9WMP7 No intermediary metabolism and respiration P64328 P9WMP7 GO:0005737,GO:0008883,GO:0055114,GO:0006779,GO:0004764,GO:0050661 1.2.1.70 P9WMP7 NC_000962.3 Mycobrowser_v5 CDS 611172 612074 . - 0 Rv0519c Rv0519c Unknown; could have possibly a lipolytic activity. Possible conserved membrane protein Rv0519c, (MTCY20G10.09c), len: 300 aa. Possible conserved membrane protein, with hydrophobic region near N-terminus. Could be a lipase. Similar to Rv0774c|MTCY369.19c|A70708 from Mycobacterium tuberculosis (312 aa), FASTA scores: opt: 1092, E(): 0, (57.9% identity in 299 aa overlap). Contains PS00120 Lipases, serine active site. O33364 No cell wall and cell processes O33364 O33364 O33364 NC_000962.3 Mycobrowser_v5 tRNA 4126541 4126614 . + 0 MTB000045 proY - tRNA-Pro (CGG) proY, tRNA-Pro, anticodon cgg, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 604602 605591 . + 0 Rv0512 hemB Involved in porphyrin and heme biosynthesis (at the second step) [catalytic activity: 2 5-aminolevulinate = porphobilinogen + 2 H2O]. Probable delta-aminolevulinic acid dehydratase HemB (porphobilinogen synthase) (ALAD) (ALADH) Rv0512, (MTCY20G10.02), len: 329 aa. Probable hemB, delta-aminolevulinic acid dehydratase, equivalent to 46723|HEM2_MYCLE delta-aminolevulinic acid dehydratase from Mycobacterium leprae (329 aa). Also highly similar to many e.g. P54919|HEM2_STRCO from Streptomyces coelicolor (330 aa); HEM2_ECOLI|P15002 from Escherichia coli (323 aa), FASTA scores: opt: 942, E(): 0, (47.6% identity in 317 aa overlap); etc. Contains PS00169 Delta-aminolevulinic acid dehydratase active site. Belongs to the ALADH family. Cofactor: zinc. P9WMP5 No intermediary metabolism and respiration O33357 P9WMP5 GO:0046872,GO:0004655,GO:0006783 4.2.1.24 P9WMP5 NC_000962.3 Mycobrowser_v5 CDS 1934482 1934649 . - 0 Rv1706A Rv1706A Function unknown Conserved hypothetical protein Rv1706A, len: 55 aa. Conserved hypothetical protein, similar to part of several probable export proteins e.g. Rv0783c|Z80226_28 from Mycobacterium tuberculosis (540 aa), FASTA scores: opt: 125, E(): 0.011, (52.85% identity in 53 aa overlap). Size difference suggests possible gene fragment. Q79FL4 No conserved hypotheticals Q79FL4 NC_000962.3 Mycobrowser_v5 CDS 608746 610056 . + 0 Rv0517 Rv0517 Function unknown; probably involved in cellular metabolism. Possible membrane acyltransferase Rv0517, (MTCY20G10.07), len: 436 aa. Possible acyltransferase, integral membrane protein, equivalent (but longer 26 aa in N-terminus) to AAK44761.1|AE006954 putative acyltransferase from Mycobacterium tuberculosis strain CDC1551 (410 aa). Also similar to many acyltransferases e.g. MDMB_STRMY|Q00718 from Streptomyces mycarofaciens (387 aa), FASTA scores: opt: 200, E(): 1.1e-08, (28.2% identity in 394 aa overlap). And similar to Rv0111, Rv0228, Rv1254, Rv1565c from Mycobacterium tuberculosis. O33362 No intermediary metabolism and respiration O33362 O33362 GO:0016020,GO:0008415 2.3.1.- NC_000962.3 Mycobrowser_v5 CDS 1530173 1530925 . + 0 Rv1359 Rv1359 Involved in transcriptional mechanism Probable transcriptional regulatory protein Rv1359, (MTCY02B10.23), len: 250 aa. Probable transcriptional regulatory protein, similar to Rv0891c|MTCY31.19c, (48.5% identity in 204 aa overlap) and to Rv1358|MTCY02B10.22 (43.7% identity in 197 aa overlap). P9WM05 No regulatory proteins P9WM05 GO:0016849,GO:0009190 P9WM05 NC_000962.3 Mycobrowser_v5 CDS 1640680 1641543 . + 0 Rv1455 Rv1455 Function unknown Conserved protein Rv1455, (MTV007.02), len: 287 aa. Conserved protein, some similarity from aa 80-160 to Z99125|MLCL536.35c hypothetical Mycobacterium leprae protein (101 aa), FASTA scores: opt: 238, E(): 1.8e-08, (51.3% identity in 78 aa overlap). O53147 No conserved hypotheticals O53147 O53147 NC_000962.3 Mycobrowser_v5 CDS 614326 614721 . - 0 Rv0523c Rv0523c Function unknown Conserved protein Rv0523c, (MTCY25D10.02), len: 131 aa. Conserved protein, showing some similarity to M. tuberculosis proteins Rv1598c|MTCY336.06; and Rv1871c|MTCY336_06|O06592 (136 aa), FASTA scores: opt: 197, E(): 5e-08, (38.4% identity in 99 aa overlap). O06389 No conserved hypotheticals O06389 O06389 NC_000962.3 Mycobrowser_v5 CDS 616223 616831 . + 0 Rv0525 Rv0525 Function unknown Conserved protein Rv0525, (MTCY25D10.04), len: 202 aa. Conserved protein, equivalent to Q49821|B2168_C3_276|S72912 hypothetical protein from Mycobacterium leprae (202 aa), FASTA scores: opt: 1151, E(): 0, (82.5% identity in 200 aa overlap). Also highly similar to CAC08377.1|AL392176 putative phosphoglycerate mutase from Streptomyces coelicolor (233 aa); and similar to SLL0395|Q55734 hypothetical 23.8 kDa protein from synechocystis SP. (212 aa), FASTA scores: opt: 207, E(): 5.1e-07, (28.2% identity in 195 aa overlap). Also some similarity to Rv2228c|Y019_MYCTU|Q10512|cy427.09 hypothetical 39.2 kDa protein from Mycobacterium tuberculosis (364 aa), FASTA scores: opt: 236, E(): 1.1e-08, (34.3% identity in 198 aa overlap). O06391 No conserved hypotheticals O06391 O06391 O06391 NC_000962.3 Mycobrowser_v5 CDS 978481 978756 . - 0 Rv0879c Rv0879c Unknown Possible conserved transmembrane protein Rv0879c, (MTCY31.07c), len: 91 aa. Possible conserved transmembrane protein, C-terminus highly similar to C-terminal part of MLCB57_14|O33059 conserved hypothetical protein from Mycobacterium leprae (91 aa), FASTA scores: E(): 1.2e-25, (76.9% identity in 91 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WKR1 No cell wall and cell processes P64735 P9WKR1 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 616846 617496 . + 0 Rv0526 Rv0526 Possibly acts on thioredoxin Possible thioredoxin protein (thiol-disulfide interchange protein) Rv0526, (MTCY25D10.05), len: 216 aa. Possible thioredoxin protein (thiol-disulfide interchange protein) , equivalent to Q49816|U2168C|S72901 hypothetical protein from Mycobacterium leprae (216 aa), FASTA scores: opt: 1144, E(): 0, (78.5% identity in 214 aa overlap). C-terminus shows some similarity to C-terminus of thioredoxins e.g. RESA_BACSU|P35160 resa protein from Bacillus subtilis (181 aa), FASTA scores: opt: 200, E(): 7.4e-06, (24.2% identity in 132 aa overlap); etc. Also similar to Mycobacterium tuberculosis thioredoxin-like proteins Rv1470, Rv1471, Rv1677, etc. Contains PS00194 Thioredoxin family active site. Seems to belong to the thioredoxin family. O06392 No intermediary metabolism and respiration O06392 O06392 GO:0045454,GO:0055114,GO:0016491,GO:0016209 1.-.-.- O06392 NC_000962.3 Mycobrowser_v5 CDS 622793 624577 . + 0 Rv0532 PE_PGRS6 Function unknown PE-PGRS family protein PE_PGRS6 Rv0532, (MTCY25D10.11), len: 594 aa. PE_PGRS6, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), similar to others e.g. Y0DP_MYCTU|Q50615 from Mycobacterium tuberculosis (498 aa), FASTA scores: opt: 1703, E(): 0, (58.2% identity in 536 aa overlap). L0T3X8 No PE/PPE Q6MX30 L0T3X8 L0T3X8 NC_000962.3 Mycobrowser_v5 CDS 1743778 1744371 . - 0 Rv1541c lprI Unknown Possible lipoprotein LprI Rv1541c, (MTCY48.24), len: 197 aa. Possible lipoprotein lprI, contains appropriately positioned prokaryotic membrane lipoprotein lipid attachment site (PS0013). P9WK41 No cell wall and cell processes P65318 P9WK41 GO:0005886 P9WK41 NC_000962.3 Mycobrowser_v5 CDS 618305 619894 . + 0 Rv0528 Rv0528 Unknown Probable conserved transmembrane protein Rv0528, (MTCY25D10.07), len: 529 aa. Probable conserved transmembrane protein, equivalent (shorter 14 aa in N-terminus) to CAC31926.1|AL583925 conserved membrane protein from Mycobacterium leprae (542 aa). Also highly similar to Q49817|B2168_C2_237|S72902 hypothetical protein from Mycobacterium leprae (364 aa), FASTA scores: opt: 1846, E(): 0, (81.1% identity in 338 aa overlap); and Q49811|B2168_C1_194|S72891 hypothetical protein from Mycobacterium leprae (106 aa), FASTA scores: opt: 506, E(): 3.8e-26, (73.6% identity in 106 aa overlap). Also highly similar to CAC08381.1|AL392176 putative integral membrane protein from Streptomyces coelicolor (574 aa). O06394 No cell wall and cell processes O06394 O06394 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 619891 620865 . + 0 Rv0529 ccsA Required during cytochrome biogenesis at the step of heme attachment. Possible cytochrome C-type biogenesis protein CcsA Rv0529, (MTCY25D10.08), len: 324 aa. Possible ccsA, cytochrome C-type biogenesis protein, integral membrane protein, equivalent to NP_302558.1|NC_002677|B2168_C3_281 possible cytochrome C biogenesis protein from Mycobacterium leprae (327 aa), FASTA scores: opt: 1779, E(): 0, (82.9% identity in 327 aa overlap). Also highly similar to others e.g. CAC08382.1|AL392176 putative cytochrome biogenesis related protein from Streptomyces coelicolor (380 aa); CCSA_CHLRE|P48269 probable cytochrome c biogenesis protein from Chlamydomonas reinhardtii (353 aa), FASTA scores: opt: 449, E(): 1.3e-23, (34.4% identity in 247 aa overlap); etc. Belongs to the CCMF/CYCK/CCL1/NRFE/CCSA family. Note that previously known as ccsB. O06393 No intermediary metabolism and respiration O06393 O06393 GO:0008535,GO:0016020 O06393 NC_000962.3 Mycobrowser_v5 CDS 620907 622124 . + 0 Rv0530 Rv0530 Function unknown Conserved protein Rv0530, (MTCY25D10.09), len: 405 aa. Conserved protein, similar in part to other hypothetical proteins e.g. AL031231|SC3C3_3|CAA20252.1 from Streptomyces coelicolor (1083 aa), FASTA scores: opt: 870, E(): 0, (39.5% identity in 443 aa overlap); etc. Also similar to Mycobacterium tuberculosis proteins e.g. Rv3868, Rv0282, Rv1798, etc. O06396 No conserved hypotheticals O06396 O06396 O06396 NC_000962.3 Mycobrowser_v5 CDS 622121 622282 . - 0 Rv0530A Rv0530A Unknown Conserved protein Rv0530A, len: 53 aa. Conserved protein. V5QPR5 No conserved hypotheticals V5QPR5 NC_000962.3 Mycobrowser_v5 CDS 650779 651504 . - 0 Rv0560c Rv0560c Possibly causes methylation Possible benzoquinone methyltransferase (methylase) Rv0560c, (MTCY25D10.39c), len: 241 aa. Possible benzoquinone methyltransferase (see citation below), similar to other hypothetical proteins and methyltransferases e.g. Q54300 methyltransferase (211 aa), FASTA scores: opt: 203, E(): 4.8e-07, (30.9% identity in 136 aa overlap). Similar to Rv3699, Rv1377c, Rv2675c, etc from Mycobacterium tuberculosis. Rv0560c can be induced by salicylate and para-amino-salicylate (pas). P9WKL5 No intermediary metabolism and respiration O06426 P9WKL5 GO:0008168,GO:0008152 2.1.1.- P9WKL5 NC_000962.3 Mycobrowser_v5 CDS 779543 780040 . - 0 Rv0679c Rv0679c Function unknown Conserved threonine rich protein Rv0679c, (MTV040.07c), len: 165 aa. Conserved Thr-rich protein, similar in part to neighboring ORF Rv0680c (124 aa), FASTA score: (35.1% identity in 131 aa overlap); and Rv0314c (220 aa). Predicted to be an outer membrane protein (See Song et al., 2008). I6WZ30 No conserved hypotheticals O53787 I6WZ30 NC_000962.3 Mycobrowser_v5 CDS 842033 842278 . + 0 Rv0750 Rv0750 Function unknown Conserved hypothetical protein Rv0750, (MTV041.24), len: 81 aa. Conserved hypothetical protein, showing almost perfect overlap with C-terminus of Rv0740|MTV041_14 conserved hypothetical protein from Mycobacterium tuberculosis (175 aa), FASTA scores: (93.8% identity in 81 aa overlap). Possible duplication. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53813 No conserved hypotheticals O53813 NC_000962.3 Mycobrowser_v5 CDS 1241971 1242831 . - 0 Rv1118c Rv1118c Function unknown Conserved protein Rv1118c, (MTCY22G8.07c), len: 286 aa. Conserved protein, similar to pseudogene ML0942 in Mycobacterium leprae. O06570 No conserved hypotheticals O06570 O06570 NC_000962.3 Mycobrowser_v5 CDS 1242864 1243013 . - 0 Rv1119c Rv1119c Unknown Hypothetical protein Rv1119c, (MTCY22G8.08c), len: 49 aa. Hypothetical unknown protein. O06571 No conserved hypotheticals O06571 O06571 NC_000962.3 Mycobrowser_v5 CDS 625562 626440 . - 0 Rv0534c menA Involved in menaquinone biosynthesis. Conversion of 1,4-dihydroxy-2-naphthoate (DHNA) to dimethylmenaquinone (DMK). Attaches octaprenylpyrophosphate, a membrane-bound 40-carbon side chain to DHNA. The conversion of DHNA to DMK proceeds in three stages: the removal of the carboxyl group of DHNA as CO2, the attachment of the isoprenoid side chain, and a quinol-to-quinone oxidation, which is thought to be spontaneous. 1,4-dihydroxy-2-naphthoate octaprenyltransferase MenA (DHNA-octaprenyltransferase) Rv0534c, (MTCY25D10.13c), len: 292 aa. Probable menA, 1,4-dihydroxy-2-naphthoate octaprenyltransferase, integral membrane protein, equivalent to Y13803|MLB1306_2|NP_302556.1 probable 4-dihydroxy-2-naphthoate octaprenyltransferase from Mycobacterium leprae (294 aa), FASTA scores: opt: 1509, E(): 0, (80.2% identity in 288 aa overlap). Also highly similar to others e.g. MENA_ECOLI|P32166|B3930 from Escherichia coli (308 aa), FASTA scores: opt: 495, E(): 2.9e-25, (36.3 identity in 289 aa overlap); etc. Belongs to the MenA family. P9WIP3 No intermediary metabolism and respiration P65650 P9WIP3 GO:0009234,GO:0005886,GO:0004659,GO:0016021 2.5.1.74 NC_000962.3 Mycobrowser_v5 CDS 661003 661269 . + 0 Rv0569 Rv0569 Function unknown Conserved protein Rv0569, (MTV039.07), len: 88 aa. Conserved protein. C-terminus highly similar to AAA63065.1|U15184|MLU15184_10 hypothetical protein from Mycobacterium leprae (53 aa), FASTA scores: opt: 140, E(): 0.0046, (64.7% identity in 34 aa overlap). Also similar to T36824|SCI35.11 hypothetical protein from Streptomyces coelicolor (64 aa); and N-terminus of T36956 probable DNA-binding protein from Streptomyces coelicolor (323 aa). Also highly similar to Rv2302|MTCY339.07c|NP_216818.1|NC_000962 conserved hypothetical protein from Mycobacterium tuberculosis (80 aa), FASTA scores: opt: 300, E(): 1.4e-13, (61.8% identity in 76 aa overlap). P9WM83 No conserved hypotheticals O53766 P9WM83 P9WM83 NC_000962.3 Mycobrowser_v5 CDS 633055 634404 . - 0 Rv0541c Rv0541c Unknown Probable conserved integral membrane protein Rv0541c, (MTCY25D10.20c), len: 449 aa. Probable conserved integral membrane protein, highly similar (except first 40 residues) to CAB76994.1|AL159178 putative integral membrane protein from Streptomyces coelicolor (456 aa). Also some similarity to Q13724|GCS1_HUMAN mannosyl-oligosaccharide glucosidase (834 aa), FASTA scores: opt: 150, E(): 0.013, (27.1% identity in 339 aa overlap). Contains PS00041 Bacterial regulatory proteins, araC family signature. O06407 No cell wall and cell processes O06407 O06407 NC_000962.3 Mycobrowser_v5 CDS 697154 697567 . - 0 Rv0598c vapC27 Unknown Possible toxin VapC27. Contains PIN domain. Rv0598c, (MTCY19H5.24), len: 137 aa. Possible vapC27, toxin, part of toxin-antitoxin (TA) operon with Rv00599c, contains PIN domain, see Arcus et al. 2005. Similar to others e.g. Rv2596|Y0B5_MYCTU|Q50625 conserved hypothetical protein from Mycobacterium tuberculosis (134 aa), FASTA scores: opt: 254, E(): 8.2e-12, (41.5% identity in 130 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WF83 No virulence, detoxification, adaptation O07780 P9WF83 P9WF83 NC_000962.3 Mycobrowser_v5 CDS 1264606 1264947 . + 0 Rv1136 Rv1136 Involved in carnitine metabolism. Possible enoyl-CoA hydratase Rv1136, (MTCI65.03), len: 113 aa. Probable enoyl-CoA hydratase (possible gene fragment). Some similarity to N-terminus of carnitine racemases and enoyl-CoA hydratases (but much shorter) e.g. I41014 carnitine racemase from Escherichia coli (297 aa), FASTA scores: opt: 258, E(): 2.5e-11, (44.5% identity in 110 aa overlap); and Rv0222 putative enoyl-CoA hydratase from M. tuberculosis (262 aa). O06536 No lipid metabolism O06536 O06536 GO:0008152,GO:0016853 5.-.-.- O06536 NC_000962.3 Mycobrowser_v5 CDS 628298 629731 . - 0 Rv0537c Rv0537c Unknown Probable integral membrane protein Rv0537c, (MTCY25D10.16c), len: 477 aa. Probable integral membrane protein, showing weak similarity to YDNK_STRCO|P40180 hypothetical 41.2 kDa protein from Streptomyces coelicolor (411 aa), FASTA scores: opt: 122, E(): 0.85, (28.2% identity in 373 aa overlap). O06403 No cell wall and cell processes O06403 O06403 NC_000962.3 Mycobrowser_v5 CDS 631743 632375 . + 0 Rv0539 Rv0539 Substrate (sugar) unknown [catalytic activity: NDP-sugar + dolichyl phosphate = NDP + dolichyl sugar phosphate]. Probable dolichyl-phosphate sugar synthase (dolichol-phosphate sugar synthetase) (dolichol-phosphate sugar transferase) (sugar phosphoryldolichol synthase) Rv0539, (MTCY25D10.18), len: 210 aa. Probable dolichol-P-sugar synthase, highly similar to CAB76989.1|AL159178 putative glycosyltransferase from Streptomyces coelicolor (242 aa), and similar to various dolichol-P-sugar synthetases and sugar transferases e.g. NP_126257.1|NC_000868 dolichyl-phosphate mannose synthase related protein from Pyrococcus abyssi (211 aa); N-terminus of NP_127133.1|NC_000868 dolichol-P-glucose synthetase from Pyrococcus abyssi (378 aa); N-terminus of NP_068880.1|NC_000917 putative dolichol-P-glucose synthetase from Archaeoglobus fulgidus (369 aa), FASTA scores: E(): 2.4e-13, (32. 1% identity in 193 aa overlap); Q26732 dolichyl-phosphate-mannose synthase precursor from trypanosoma brucei (267 aa), FASTA scores: opt: 179, E(): 0.0011, (30.7% identity in 205 aa overlap); etc. Also similar to Rv2051c|MTY25D10_18 from Mycobacterium tuberculosis. Contains PS00017 ATP/GTP-binding site motif A (P-loop). P9WMY1 No intermediary metabolism and respiration P0A597 P9WMY1 GO:0016757 2.4.-.- P9WMY1 NC_000962.3 Mycobrowser_v5 CDS 635573 635875 . - 0 Rv0543c Rv0543c Function unknown Conserved protein Rv0543c, (MTCY25D10.22c), len: 100 aa. Conserved protein, equivalent to Q50171|MLU15187_32|NP_302469.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (100 aa), FASTA scores: opt: 493, E(): 6.1e-30, (73.5% identity in 98 aa overlap). Some similarity to Rv3046c|NP_217562.1 from Mycobacterium tuberculosis. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Alternative nucleotide at position 635633 (C->T; A81A) has been observed. O06409 No conserved hypotheticals O06409 O06409 O06409 NC_000962.3 Mycobrowser_v5 CDS 637583 637969 . - 0 Rv0546c Rv0546c Function unknown Conserved protein Rv0546c, (MTCY25D10.25c), len: 128 aa. Conserved protein, equivalent to AAA63111.1|U15187|Q50174|U296X hypothetical protein from Mycobacterium leprae (144 aa), FASTA scores: opt: 748, E(): 0, (84.2% identity in 133 aa overlap). Also highly similar to CAB95979.1|AL360034 conserved hypothetical protein from Streptomyces coelicolor (130 aa); and similar to AE000854_8|O26852 S-D-lactoylglutathione methylglyoxal lyase from Methanobacterium thermoautotropto (116 aa), FASTA scores: opt: 155, E(): 0.00019, (30.6% identity in 108 aa overlap); YAER_ECOLI hypothetical 14.7 kDa protein from Escherichia coli (129 aa), FASTA scores: opt: 104, E(): 0.42, (28.7% identity in 115 aa overlap). Also similar to Rv2068c from Mycobacterium tuberculosis. O06412 No conserved hypotheticals O06412 O06412 O06412 NC_000962.3 Mycobrowser_v5 CDS 817531 817866 . - 0 Rv0724A Rv0724A Function unknown Conserved hypothetical protein Rv0724A, len: 111 aa. Similarity suggests that this CDS should be continuation of Rv0725c but we can find no frame-shift to account for this. Possible extended protein is very similar to other hypothetical Mycobacterium tuberculosis proteins e.g. Rv1729c|Z81360_12 (312 aa), FASTA scores: opt: 399, E(): 2e-19, (58.7% identity in 109 aa overlap); Rv0731c, Rv0726c, etc. Frame-shift could occur at nt 817866. Same sequence for strain CDC1551 and Mycobacterium bovis. Q79FX1 No conserved hypotheticals Q79FX1 Q79FX1 NC_000962.3 Mycobrowser_v5 CDS 638032 638916 . - 0 Rv0547c Rv0547c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0547c, (MTCY25D10.26c), len: 294 aa. Possible oxidoreductase, similar to various oxidoreductases e.g. fatty acyl-CoA reductase from Acinetobacter calcoaceticus (295 aa); NP_280196.1|NC_002607 3-oxoacyl-[acyl-carrier-protein] reductase from Halobacterium sp. NRC-1 (255 aa); NP_349214.1|NC_003030 Short-chain alcohol dehydrogenase family protein from Clostridium acetobutylicum (255 aa); etc. Also similar to several proteins from Mycobacterium tuberculosis e.g. Y04M_MYCTU|Q10783 putative oxidoreductase (341 aa), FASTA scores: opt: 644, E(): 0, (46.1% identity in 258 aa overlap). O06413 No intermediary metabolism and respiration O06413 O06413 GO:0055114,GO:0016491 1.-.-.- O06413 NC_000962.3 Mycobrowser_v5 CDS 639012 639956 . - 0 Rv0548c menB Involved in menaquinone biosynthesis. Convert O-succinylbenzoyl-CoA (OSB-CoA) to 1,4-dihydroxy-2-naphthoic acid (DHNA) [catalytic activity: O-succinylbenzoyl-CoA = 1,4-dihydroxy-2-naphthoate + CoA]. Naphthoate synthase MenB (dihydroxynaphthoic acid synthetase) (DHNA synthetase) Rv0548c, (MTCY25D10.27c), len: 314 aa. menB, naphthoate synthase (dihydroxynaphthonic acid synthase), equivalent to NP_302473.1|NC_002677 naphthoate synthase from Mycobacterium leprae (300 aa). Also similar to others e.g. MENB_ECOLI|P27290 naphthoate synthase from Escherichia coli (285 aa), FASTA scores: opt: 599, E(): 9.3e-33, (48.1 identity in 285 aa overlap); etc. Belongs to the enoyl-CoA hydratase/isomerase family. P9WNP5 No intermediary metabolism and respiration 1Q51,1Q52,1RJM,1RJN,3T8A,3T8B O06414 P9WNP5 GO:0008935,GO:0009234 4.1.3.36 P9WNP5 NC_000962.3 Mycobrowser_v5 CDS 640228 640641 . - 0 Rv0549c vapC3 Unknown Possible toxin VapC3 Rv0549c, (MTCY25D10.28c), len: 137 aa. Possible vapC3, toxin, part of toxin-antitoxin (TA) operon with Rv0550c, contains PIN domain (see Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others e.g. Rv0960, Rv0065, and Rv1720c from Mycobacterium tuberculosis. P9WFB7 No virulence, detoxification, adaptation O06415 P9WFB7 P9WFB7 NC_000962.3 Mycobrowser_v5 CDS 640638 640904 . - 0 Rv0550c vapB3 Unknown Possible antitoxin VapB3 Rv0550c, (MTCY25D10.29c), len: 88 aa. Possible vapB3, antitoxin, part of toxin-antitoxin (TA) operon with Rv0549c (See Arcus et al., 2005; Pandey and Gerdes, 2005). P9WJ59 No virulence, detoxification, adaptation O06416 P9WJ59 NC_000962.3 Mycobrowser_v5 CDS 658321 659340 . + 0 Rv0567 Rv0567 Causes methylation Probable methyltransferase/methylase Rv0567, (MTV039.05), len: 339 aa. Probable methyltransferase, similar to several e.g. P39896|TCMO_STRGA tetracenomycin polyketide synthesis 8-O-methyltransferase from Streptomyces glaucescens (339 aa), FASTA scores: opt: 685, E(): 0, (35.8% identity in 335 aa overlap); P10950|HIOM_BOVIN hydroxyindole O-methyltransferase from Bos taurus (345 aa), FASTA scores: opt: 509, E(): 3.4e-27, (30.7% identity in 332 aa overlap) etc. O53764 No intermediary metabolism and respiration O53764 O53764 GO:0008171 2.1.1.- O53764 NC_000962.3 Mycobrowser_v5 CDS 642889 644493 . + 0 Rv0552 Rv0552 Function unknown Conserved protein Rv0552, (MTCY25D10.31), len: 534 aa. Conserved protein, similar to others from several organisms. Also shows some similarity with regulatory proteins e.g. AEPA_ERWCA|Q06555 exoenzymes regulatory protein aepA [Precursor] from Erwinia carotovora (465 aa), FASTA scores: opt: 278, E(): 7.6e-11, (23.0% identity in 408 aa overlap). Also similar to Z99119|BSUB0016_28 from Bacillus subtilis (529 aa), FASTA scores: opt: 436, E(): 8.3e-20, (23.8% identity in 547 aa overlap). C-terminus is similar to MLRRNOPR_1 hypothetical 17.7 kDa protein from Mycobacterium leprae (154 aa), FASTA score: (43.1% identity in 160 aa overlap). O06418 No conserved hypotheticals O06418 O06418 GO:0016810 O06418 NC_000962.3 Mycobrowser_v5 CDS 644490 645470 . + 0 Rv0553 menC Possibly involved in menaquinone biosynthesis. Catalyzes a syn cycloisomerization [catalytic activity: 2,5-dihydro-5-oxofuran-2-acetate = cis,cis-hexadienedioate]. Probable muconate cycloisomerase MenC (cis,cis-muconate lactonizing enzyme) (MLE) Rv0553, (MTCY25D10.32), len: 326 aa. Probable menC, muconate cycloisomerase, equivalent to NP_302476.1|NC_002677 putative isomerase/racemase from Mycobacterium leprae (334 aa). Also similar to other muconate cycloisomerases e.g. TCBD_PSESP|P27099 chloromuconate cycloisomerase (370 aa), FASTA scores: opt: 249, E(): 7.8e-09, (32.7% identity in 199 aa overlap). Also similar to O-succinylbenzoate-CoA synthases. Belongs to the mandelate racemase / muconate lactonizing enzyme family. P9WJP3 No intermediary metabolism and respiration P65425 P9WJP3 GO:0009234,GO:0046872,GO:0016836 4.2.1.113 P9WJP3 NC_000962.3 Mycobrowser_v5 CDS 698524 698994 . - 0 Rv0601c Rv0601c Sensor part of a two component regulatory system. Two component sensor kinase [first part] Rv0601c, (MTCY19H5.21), len: 156 aa (probable partial CDS). Two-component sensor kinase (first part), similar to part (N-termini) of others e.g. Q0375|CUTS_STRLI cuts protein from streptomyces lividans (414 aa), FASTA scores: opt: 230, E(): 3.1e-08, (39.1% identity in 115 aa overlap). Note that the sequence was checked and no errors were detected that would allow this and the downstream ORF to be joined. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07777 No regulatory proteins O07777 O07777 GO:0005886,GO:0007165,GO:0000155,GO:0000160,GO:0016021 2.7.13.3 NC_000962.3 Mycobrowser_v5 CDS 645467 646255 . + 0 Rv0554 bpoC Supposedly involved in detoxification reactions. Possible peroxidase BpoC (non-haem peroxidase) Rv0554, (MTCY25D10.33), len: 262 aa. Possible bpoC, peroxidase (non-haem peroxidase), equivalent to NP_302477.1|NC_002677 putative hydrolase from Mycobacterium leprae (265 aa). Also highly similar or similar to various hydrolases and peroxidases e.g. CAB38877.1|AL035707|T36181 probable hydrolase from Streptomyces coelicolor (272 aa); CAC48368.1|Y16952 putative hydrolase from Amycolatopsis mediterranei (284 aa); P29715|BPA2_STRAU non-haem bromoperoxidase bpo-a2 (bromide peroxidase) from Streptomyces aureofaciens (277 aa), FASTA scores: opt: 325, E(): 2.3e-15, (29.5% identity in 268 aa overlap); O31168|PRXC_STRAU|CPO|CPOT non-heme chloroperoxidase (chloride peroxidase) from Streptomyces aureofaciens (278 aa); etc. Also similar to M. tuberculosis non-heme haloperoxidases and epoxide hydrolases e.g. Rv1938, Rv3617, etc. P9WNH1 No virulence, detoxification, adaptation 3E3A,3HSS,3HYS,3HZO O06420 P9WNH1 GO:0004601,GO:0055114 1.11.1.- P9WNH1 NC_000962.3 Mycobrowser_v5 CDS 651529 652755 . - 0 Rv0561c Rv0561c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0561c, (MTCY25D10.40c), len: 408 aa. Possible oxidoreductase, highly similar (except in first 30 aa) to NP_302482.1|NC_002677 putative FAD-linked oxidoreductase from Mycobacterium leprae (408 aa). Also similar to T34627 probable electron transfer oxidoreductase from Streptomyces coelicolor (430 aa); and some bacteriochlorophyll synthases e.g. NP_069300.1|NC_000917 bacteriochlorophyll synthase from Archaeoglobus fulgidus (410 aa); Q55087 geranylgeranyl hydrogenase (407 aa), FASTA scores: opt: 208, E(): 1.7e-06, (26.9% identity in 327 aa overlap). P9WNY9 No intermediary metabolism and respiration O06427 P9WNY9 GO:0055114,GO:0004497 1.-.-.- P9WNY9 NC_000962.3 Mycobrowser_v5 CDS 677125 677616 . - 0 Rv0580c Rv0580c Function unknown Conserved protein Rv0580c, (MTV039.18c), len: 163 aa. Conserved protein, equivalent to AAA90989.1|U20446|MK35 lipoprotein precursor from Mycobacterium kansasii (225 aa). O53777 No conserved hypotheticals O53777 O53777 NC_000962.3 Mycobrowser_v5 CDS 1214513 1214947 . + 0 Rv1088 PE9 Function unknown PE family protein PE9 Rv1088, (MTV017.41), len: 144 aa. PE9, Member of Mycobacterium tuberculosis PE family (see citation below), similar to many others e.g. Z96071|MTCI418B_6 Mycobacterium tuberculosis cosmid (487 aa), FASTA scores: opt: 318, E(): 7.3e-14, (60.9% identity in 87 aa overlap) - except it appears to be frameshifted around codon 84. No error to account for frameshift could be found. Q79FS8 No PE/PPE Q79FS8 Q79FS8 Q79FS8 NC_000962.3 Mycobrowser_v5 CDS 1265087 1265455 . - 0 Rv1137c Rv1137c Unknown Hypothetical protein Rv1137c, (MTCI65.04c), len: 122 aa. Hypothetical unknown protein. O06537 No conserved hypotheticals O06537 NC_000962.3 Mycobrowser_v5 CDS 647959 648474 . + 0 Rv0556 Rv0556 Unknown Probable conserved transmembrane protein Rv0556, (MTCY25D10.35), len: 171 aa. Probable conserved transmembrane protein, equivalent to NP_302479.1|NC_002677 putative membrane protein from Mycobacterium leprae (175 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06422 No cell wall and cell processes O06422 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 648536 649672 . + 0 Rv0557 mgtA Involved in lipomannan (LM) biosynthesis Mannosyltransferase MgtA Rv0557, (MTCY25D10.36), len: 378 aa. MgtA (previously known as pimB), mannosyltransferase (see citation below), similar to other various transferases e.g. NP_243554.1|NC_002570 alpha-D-mannose-alpha(1-6)phosphatidyl myo-inositol monomannoside transferase from Bacillus halodurans (381 aa); NP_249533.1|NC_002516 probable glycosyl transferase from Pseudomonas aeruginosa (406 aa); NP_419573.1|NC_002696 glycosyl transferase, group 1 family protein, from Caulobacter crescentus (455 aa); etc. Also similar to Q55598 hypothetical 44.9 kDa protein from synechocystis SP (409 aa), FASTA scores: opt: 703, E(): 0, (33.9% identity in 378 aa overlap); GPI3_YEAST|P32363 n-acetylglucosaminyl-phosphatidylinositol biosynthetic protein (452 aa), FASTA scores: opt: 230, E(): 1.1e-07, (23.5% identity in 328 aa overlap). P9WMY5 No lipid metabolism O06423 P9WMY5 GO:0008654,GO:0016757,GO:0009405 2.4.1.- P9WMY5 NC_000962.3 Mycobrowser_v5 CDS 652771 653778 . + 0 Rv0562 grcC1 Possibly supplies polyprenyl diphosphate. Probable polyprenyl-diphosphate synthase GrcC1 (polyprenyl pyrophosphate synthetase) Rv0562, (MTCY25D10.41), len: 335 aa. Probable grcC1, polyprenyl diphosphate synthetase, equivalent to NP_302483.1|NC_002677 polyprenyl diphosphate synthase component from Mycobacterium leprae (330 aa). Also similar to others (generally hepta or hexaprenyl) e.g. GRC3_BACSU|P31114 probable heptaprenyl diphosphate syntetase (348 aa), FASTA scores: opt: 599, E(): 4e-31, (33.2% identity in 307 aa overlap); etc. Also highly similar to Mycobacterium tuberculosis proteins Rv0989c|grcC2|NP_215504.1|MTCI237.03c probable polyprenyl-diphosphate synthase (325 aa); Rv3383c, Rv3398c, etc. Contains PS00444 Polyprenyl synthetases signature 2. Belongs to the FPP/GGPP synthetases family. O06428 No intermediary metabolism and respiration O06428 O06428 GO:0016740,GO:0008299 2.5.1.- O06428 NC_000962.3 Mycobrowser_v5 CDS 1419961 1420302 . - 0 Rv1271c Rv1271c Unknown Conserved hypothetical secreted protein Rv1271c, (MTCY50.11), len: 113 aa. Conserved hypothetical exported protein with potential N-terminal signal sequence. Similar to Mycobacterium tuberculosis hypothetical proteins Rv1804c, Rv1810, Rv0622, etc. P9WM43 No cell wall and cell processes P64793 P9WM43 P9WM43 NC_000962.3 Mycobrowser_v5 CDS 654924 655949 . - 0 Rv0564c gpdA1 Involved in de novo phospholipid biosynthesis; glycerol-3 phosphate formation [catalytic activity: SN-glycerol 3-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H]. Probable glycerol-3-phosphate dehydrogenase [NAD(P)+] GpdA1 (NAD(P)H-dependent glycerol-3-phosphate dehydrogenase) (NAD(P)H-dependent dihydroxyacetone-phosphate reductase) Rv0564c, (MTV039.02c), len: 341 aa. Possible gpdA1(alternate gene names: gpsA, glyC), glycerol-3-phosphate dehydrogenase [NAD(P)+] dependent, similar to many other glycerol-3-phosphate dehydrogenases e.g. P46919|GPDA_BACSU from Bacillus subtilis (345 aa), FASTA scores: opt: 731, E(): 0, (37.3% identity in 332 aa overlap); etc. Also similar to Rv2982c|gpdA2|MTCY349.05|Z83018|MTCY349_5 from Mycobacterium tuberculosis (334 aa), FASTA scores: opt: 740, E(): 0, (40.4% identity in 322 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family. P9WN75 No lipid metabolism P64188 P9WN75 GO:0046167,GO:0046168,GO:0004367,GO:0047952,GO:0009331,GO:0055114,GO:0008654,GO:0051287 1.1.1.94 P9WN75 NC_000962.3 Mycobrowser_v5 CDS 656010 657470 . - 0 Rv0565c Rv0565c Function unknown; probably involved in cellular metabolism. Probable monooxygenase Rv0565c, (MTV039.03c), len: 486 aa. Probable monoxygenase, highly similar to NP_301173.1|NC_002677 putative monooxygenase from Mycobacterium leprae (494 aa). Also highly similar to others e.g. NP_421371.1|NC_002696 monooxygenase (flavin-binding family) from Caulobacter crescentus (498 aa); C-terminus of NP_051574.1|NC_000958 arylesterase/monoxygenase from Deinococcus radiodurans (833 aa); P12015|CYMO_ACISP cyclohexanone monooxygenase from Acinetobacter sp. (542 aa), FASTA scores: opt: 354, E(): 2.1e-16, (23.7% identity in 435 aa overlap); etc. Also similar to other putative monoxygenases from Mycobacterium tuberculosis e.g. Rv3854c (489 aa), MTCY01A6.14 (489 aa), MTV013_4 (495 aa), MTCY31.20 (495 aa). O53762 No intermediary metabolism and respiration O53762 O53762 GO:0050661,GO:0004499,GO:0055114,GO:0050660 1.14.-.- O53762 NC_000962.3 Mycobrowser_v5 CDS 657548 658039 . - 0 Rv0566c Rv0566c Function unknown Conserved protein Rv0566c, (MTV039.04c), len: 163 aa. Conserved protein, similar to others e.g. P77482|YAJQ_ECOLI hypothetical 19.0 KDa protein from Escherichia coli (169 aa), FASTA scores: opt: 422, E(): 5.4e-20, (44.1 identity in 161 aa overlap); etc. P9WFK9 No conserved hypotheticals P67380 P9WFK9 P9WFK9 NC_000962.3 Mycobrowser_v5 CDS 667252 668394 . - 0 Rv0574c Rv0574c Function unknown Conserved hypothetical protein Rv0574c, (MTV039.12c), len: 380 aa. Conserved hypothetical protein, showing similarity with other hypothetical proteins and polyglutamate synthases (encapsulation proteins) e.g. AAK64444.1|AF377339_5|AF377339 polyglutamate synthase CapA from Myxococcus xanthus (405 aa); M24150|BACCAPABC_3|CapA polyglutamate synthase (encapsulation protein) from B.anthracis (411 aa), FASTA scores: opt: 261, E(): 4.3e-10, (25.8% identity in 287 aa overlap); etc. P9WM79 No conserved hypotheticals O53771 P9WM79 P9WM79 NC_000962.3 Mycobrowser_v5 CDS 676238 676996 . + 0 Rv0579 Rv0579 Function unknown Conserved hypothetical protein Rv0579, (MTV039.17), len: 252 aa. Conserved hypothetical protein, showing some similarity to others e.g. AE001747_4 hypothetical protein from Thermotoga maritima (247 aa), FASTA scores: opt: 612, E(): 0, (39.6% identity in 235 aa overlap); AE001004_2 hypothetical protein from Archaeoglobus fulgidus (159 aa), FASTA scores: opt: 196, E(): 1e-06, (28.3% identity in 159 aa overlap); etc. O53776 No conserved hypotheticals O53776 O53776 O53776 NC_000962.3 Mycobrowser_v5 CDS 2546488 2546805 . - 0 Rv2274c mazF8 Unknown Possible toxin MazF8 Rv2274c, (MTCY339.36), len: 105 aa. Possible mazF8, toxin, part of toxin-antitoxin (TA) operon with Rv2274A (See Pandey and Gerdes, 2005). Questionable ORF. P9WIH7 No virulence, detoxification, adaptation P9WIH7 P9WIH7 NC_000962.3 Mycobrowser_v5 CDS 663487 664818 . - 0 Rv0571c Rv0571c Function unknown Conserved protein Rv0571c, (MTV039.09c), len: 443 aa. Conserved protein, highly similar to the products of two adjacent orfs in Mycobacterium leprae: AAA63059.1|U15184|U650S|Q50111 hypothetical protein (258 aa), FASTA scores: opt: 1071, E(): 0, (72.5% identity in 233 aa overlap); and AAA63058.1|U15184|U650T hypothetical protein (86 aa), FASTA scores: opt: 192, E(): 6.4e-06, (70.8% identity in 48 aa overlap). Also similar to others e.g. NP_107072.1|NC_002678 hypothetical protein from Mesorhizobium loti (235 aa); NP_213031.1|NC_000918 hypothetical protein from Aquifex aeolicus (175 aa); etc. And similar to part of hypothetical proteins from Mycobacterium tuberculosis e.g. C-terminus of Rv2143|MTCY270.25c|Z95388|NP_216659.1|NC_000962 (352 aa), FASTA scores: opt: 592, E(): 7e-32, (49.3% identity in 205 aa overlap); N-terminus of Rv2030c|NP_216546.1|NC_000962 (681 aa). P9WHK1 No conserved hypotheticals O53768 P9WHK1 GO:0009116,GO:0016740,GO:0016787 P9WHK1 NC_000962.3 Mycobrowser_v5 CDS 679229 681862 . + 0 Rv0584 Rv0584 Unknown Possible conserved exported protein Rv0584, (MTV039.22), len: 877 aa. Possible conserved exported protein, similar to other hypothetical proteins which are not necessarily secreted e.g. CAB61925.1|AL133278 putative secreted protein from Streptomyces coelicolor (772 aa); AAD51075.1|AF175722_1|AF175722 immunoreactive 89kD antigen PG87 from Porphyromonas gingivalis (781 aa), FASTA scores: opt: 637, E(): 2.1e-30, (29.1% identity in 794 aa overlap); etc. Contains PS00699 Nitrogenases component 1 alpha and beta subunits signature 1. Has potential N-terminal signal peptide. Predicted to be an outer membrane protein (See Song et al., 2008). O86365 No cell wall and cell processes O86365 O86365 O86365 NC_000962.3 Mycobrowser_v5 CDS 668579 669745 . - 0 Rv0575c Rv0575c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0575c, (MTV039.13c), len: 388 aa. Possible oxidoreductase, similar to many diverse oxidoreductases and monooxygenases e.g. AL109974|SCF34_5|T36404 probable monooxygenase from Streptomyces coelicolor (407 aa), FASTA scores: opt: 786, E(): 0, (38.7% identity in 398 aa overlap); P96555|AB000564 salicylate hydroxylase from sphingomonas (395 aa), FASTA scores: opt: 267, E():5e-11, (26.4% identity in 390 aa overlap). Also similar to Rv1260|Z77137|MTCY50.22C from Mycobacterium tuberculosis (372 aa), FASTA scores: opt: 762, E(): 0, (40.9% identity in 345 aa overlap). The transcription of this CDS seems to be activated in macrophages (see citation below). O53772 No intermediary metabolism and respiration O53772 O53772 GO:0055114,GO:0004497 1.-.-.- O53772 NC_000962.3 Mycobrowser_v5 CDS 669848 671152 . + 0 Rv0576 Rv0576 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (possibly ArsR-family) Rv0576, (MTV039.14), len: 434 aa. Probable transcriptional regulator, ArsR family. N-terminus highly similar to others e.g. NP_102487.1|NC_002678 transcriptional regulator from Mesorhizobium loti (104 aa); NP_242952.1|NC_002570 transcriptional regulator (ArsR family) from Bacillus halodurans (109 aa); etc. C-terminal region (~240-434) shows similarity with D67028_1 from Rhodococcus rhodochrous (112 aa); and Rv0738 from Mycobacterium tuberculosis (182 aa). N-terminus also highly similar to Rv2034 from Mycobacterium tuberculosis (107 aa). Contains helix-turn-helix motif at aa 23-43 (Score 1628, +4.73 SD). O53773 No regulatory proteins O53773 O53773 GO:0006355,GO:0006950,GO:0006350,GO:0003700,GO:0005622 O53773 NC_000962.3 Mycobrowser_v5 CDS 671166 671951 . + 0 Rv0577 TB27.3 Function unknown. Involved in ability to absorb neutral red stain. Conserved protein TB27.3 Rv0577, (MTV039.15), len: 261 aa. TB27.3, conserved protein. Corresponds to O53774|CF30_MYCTU 27 kDa antigen CFP30B from Mycobacterium tuberculosis culture filtrate (260 aa), FASTA scores: opt: 1781, E(): 0, (100.0% identity in 260 aa overlap). Also similar to several hypothetical proteins and hydroxylases from Steptomyces sp. e.g. T35032 probable hydroxylase from Streptomyces coelicolor (263 aa); Q55078 orfA gene product from Streptomyces sp. (275 aa), FASTA scores: E(): 1.5e-1 9, (38.6% identity in 264 aa overlap); D89734_1|P95754 DNA for SgaA SGAA protein from Streptomyces griseus; and SC9B10_20 from Streptomyces coelicolor (267 aa), FASTA score: (38.9 identity in 252 aa overlap). Also similar to Rv0911|MTCY21C12.05 from Mycobacterium tuberculosis (257 aa), FASTA scores: E(): 1.1e-20, (32.0% identity in 259 aa overlap). P9WIR3 No conserved hypotheticals 3OXH P0A5N8 P9WIR3 P9WIR3 NC_000962.3 Mycobrowser_v5 CDS 1981130 1981579 . + 0 Rv1752 Rv1752 Function unknown Conserved hypothetical protein Rv1752, (MTCY28.15), len: 149 aa. Conserved hypothetical protein, similar to C-terminal half of Q9TV68|AB021930|CAN2DD Dihydrodiol dehydrogenase from Canis familiaris (335 aa), FASTA score, opt: 168, E(): 0.00015, (31.3% identity in 112 aa overlap). O06789 No conserved hypotheticals O06789 O06789 NC_000962.3 Mycobrowser_v5 CDS 684410 685132 . + 0 Rv0586 mce2R Involved in transcriptional mechanism. Probable transcriptional regulatory protein Mce2R (GntR-family) Rv0586, (MTCY19H5.36c), len: 240 aa. Probable mce2R, transcriptional regulator, GntR family, part of mce2 operon, similar to many e.g. P33233|LLDR_ECOLI putative L-lactate dehydrogenase operon regulatory protein from Escherichia coli (258 aa), FASTA scores: opt: 225, E(): 9.3e-08, (26.7% identity in 232 aa overlap); etc. Also similar to other M. tuberculosis transcriptional regulators GntR proteins e.g. Rv3060c, Rv0792c, etc. Contains PS00043 Bacterial regulatory proteins, gntR family signature and probable helix-turn helix motif from aa 35-56 (Score 1531, +4.40 SD). P9WMG5 No regulatory proteins P67741 P9WMG5 GO:0006355,GO:0006350,GO:0003700,GO:0005622 P9WMG5 NC_000962.3 Mycobrowser_v5 CDS 685129 685926 . + 0 Rv0587 yrbE2A Unknown Conserved hypothetical integral membrane protein YrbE2A Rv0587, (MTCY19H5.35c), len: 265 aa. YrbE2A, hypothetical unknown integral membrane protein, part of mce2 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07412|Rv0167|MTCI28.07|yrbE1A (265 aa); O53965|Rv1964|MTV051.02|yrbE3A (265 aa); etc. Also highly similar to conserved hypothetical integral membrane proteins of the yrbEA type, e.g. P45392|YRBE_ECOLI hypothetical 27.9 kDa protein from Escherichia coli (260 aa), FASTA scores: opt: 287, E(): 6.1e-12, (21.5% identity in 256 aa overlap); P45030|YRBE_HAEIN|HI1086 hypothetical protein from Haemophilus influenzae (261 aa), FASTA scores: opt: 311, E(): 1.8e-83, (24.2% identity in 265 aa overlap); NP_302654.1|NC_002677 conserved membrane protein from Mycobacterium leprae (267 aa); etc. I6Y870 No virulence, detoxification, adaptation O07791 I6Y870 NC_000962.3 Mycobrowser_v5 CDS 699038 699799 . - 0 Rv0602c tcrA Sensor part of a two component regulatory system. Two component DNA binding transcriptional regulatory protein TcrA Rv0602c, (MTCY19H5.20), len: 253 aa. tcrA, two-component DNA-binding response regulator, highly similar to others e.g. NP_107959.1|NC_002678 two-component response regulator from Mesorhizobium loti (239 aa); etc. Also similar to many other Mycobacterium tuberculosis two-component regulators e.g. Q50806|MTCY10G2.16|Rv1033c response regulator homolog TRCR (TCRV) (257 aa), FASTA score: (47.4 identity in 232 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07776 No regulatory proteins O07776 O07776 GO:0005737,GO:0006355,GO:0006350,GO:0000156,GO:0000160,GO:0003677 O07776 NC_000962.3 Mycobrowser_v5 CDS 702813 703199 . + 0 Rv0607 Rv0607 Unknown Hypothetical protein Rv0607, (MTCY19H5.15c), len: 128 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07771 No conserved hypotheticals O07771 NC_000962.3 Mycobrowser_v5 CDS 703244 703489 . + 0 Rv0608 vapB28 Unknown Possible antitoxin VapB28 Rv0608, (MTCY19H5.14c), len: 81 aa. Possible vapB28, antitoxin, part of toxin-antitoxin (TA) operon with Rv0609, see Arcus et al. 2005. Similar to several others e.g. Rv0623|P96913|MTCY20H10.04 (84 aa), FASTA scores: opt: 159, E(): 1.2e-09, (43.0% identity in 86 aa overlap); Rv2760c (89 aa); Rv1740 (70 aa), etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WJ39 No virulence, detoxification, adaptation O07770 P9WJ39 NC_000962.3 Mycobrowser_v5 CDS 703830 704057 . + 0 Rv0609A Rv0609A Function unknown Conserved hypothetical protein Rv0609A, len: 75 aa. Conserved hypothetical protein, highly similar to part of upstream ORF Rv0612|MTCY19H5.09c conserved hypothetical protein from Mycobacterium tuberculosis (201 aa), FASTA scores: opt: 154, E(): 1.8e-05, (74.3% identity in 35 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q79FY5 No conserved hypotheticals Q79FY5 NC_000962.3 Mycobrowser_v5 ncRNA 704187 704247 . + 0 MTB000052 B55 Unknown Putative small regulatory RNA B55, putative small regulatory RNA (See Arnvig and Young, 2009). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 711006 711407 . + 0 Rv0617 vapC29 Unknown Possible toxin VapC29. Contains PIN domain. Rv0617, (MTCY19H5.04c), len: 133 aa. Possible vapC29, toxin, part of toxin-antitoxin (TA) operon with Rv0616A, contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. Rv2494, Rv3320c, Rv0749, Rv0277c, Rv2530c, etc. P9WF79 No virulence, detoxification, adaptation O07760 P9WF79 P9WF79 NC_000962.3 Mycobrowser_v5 CDS 711536 712231 . + 0 Rv0618 galTa Involved in galactose metabolism (leloir pathway) [catalytic activity: UTP + alpha-D-galactose 1-phosphate = diphosphate + UDP-galactose]. Probable galactose-1-phosphate uridylyltransferase GalTa [first part] Rv0618, (MTCY19H5.03c), len: 231 aa (probable partial CDS). Probable galTa, first part of galactose-1-phosphate uridylyltransferase, highly similar to N-terminal half of other galT proteins e.g. P13212|GAL7_STRLI galactose-1-phosphate uridylyltransferase from Streptomyces lividans (354 aa), FASTA scores: opt: 296, E(): 1.4e-11, (50.8% identity in 177 aa overlap); etc. Also highly similar to N-terminal half of some UDP glucose--hexose-1-phosphate uridylyltransferases. N-terminal 28 aa similar to MTCY20H11.08|Rv0627|MTCY20H11.08 conserved hypothetical protein from Mycobacterium tuberculosis (135 aa), FASTA score: (71.4% identity in 28 overlap). Cosmid sequence is correct but there may be a frameshift mutation in this region which would allow the two ORFs to be joined. Belongs to the galactose-1-phosphate uridylyltransferase family 1. Note that previously known as galT'. Q79FY4 No intermediary metabolism and respiration Q79FY4 Q79FY4 GO:0017103,GO:0006012,GO:0008270,GO:0008108 2.7.7.10 Q79FY4 NC_000962.3 Mycobrowser_v5 CDS 712174 712719 . + 0 Rv0619 galTb Involved in galactose metabolism (leloir pathway) [catalytic activity: UTP + alpha-D-galactose 1-phosphate = diphosphate + UDP-galactose]. Probable galactose-1-phosphate uridylyltransferase GalTb [second part] Rv0619, (MTCY19H5.02c), len: 181 aa (probable partial CDS). Probable galTb, second part of galactose-1-phosphate uridylyltransferase, highly similar to C-terminal half of other galT proteins e.g. P13212|GAL7_STRLI galactose-1-phosphate uridylyltransferase from Streptomyces lividans (354 aa), FASTA scores: opt: 416, E(): 5.2e-22, (43.0% identity in 186 aa overlap), etc. Cosmid sequence is correct but there may be a frameshift mutation in this region which would allow the two ORFS to be joined. Belongs to the galactose-1-phosphate uridylyltransferase family 1. Note that previously known as 'galT. Q79FY3 No intermediary metabolism and respiration Q79FY3 Q79FY3 GO:0017103,GO:0006012,GO:0008270,GO:0008108 2.7.7.10 Q79FY3 NC_000962.3 Mycobrowser_v5 CDS 729327 730166 . - 0 Rv0633c Rv0633c Unknown Possible exported protein Rv0633c, (MTCY20H11.14c), len: 279 aa. Possible exported protein; has hydrophobic stretch at aa 23-41. P96923 No cell wall and cell processes P96923 NC_000962.3 Mycobrowser_v5 CDS 828140 828892 . + 0 Rv0736 rslA Regulates negatively SIGL|Rv0735 Anti-sigma factor RslA Rv0736, (MTV041.10), len: 250 aa. RslA, anti-sigma factor (See Dainese et al., 2006). Probable membrane protein, showing weak similarity with AL133469|SCM10_32 putative membrane protein from Streptomyces coelicolor (216 aa), FASTA scores: opt: 180, E(): 0.00018, (34.3% identity in 216 aa overlap). Cleaved by Rip|Rv2869c, in M. tuberculosis Erdman (See Sklar et al., 2010). P9WJ67 No information pathways 3HUG P9WJ67 P9WJ67 NC_000962.3 Mycobrowser_v5 CDS 1445058 1445372 . + 0 Rv1290A Rv1290A Unknown Hypothetical protein Rv1290A, len: 104 aa. Hypothetical unknown protein, equivalent to AAK45590 from Mycobacterium tuberculosis strain CDC1551 (122 aa) but shorter 18 aa. Q79FQ6 No conserved hypotheticals Q79FQ6 NC_000962.3 Mycobrowser_v5 CDS 718282 718689 . + 0 Rv0627 vapC5 Unknown Possible toxin VapC5 Rv0627, (MTCY20H11.08), len: 135 aa. Possible vapC5, toxin, part of toxin-antitoxin (TA) operon with Rv0626, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. Rv0595c and Rv0665. P96917 No virulence, detoxification, adaptation 3DBO P96917 P96917 P96917 NC_000962.3 Mycobrowser_v5 CDS 714202 715266 . + 0 Rv0621 Rv0621 Unknown Possible membrane protein Rv0621, (MTCY20H10.02), len: 354 aa. Possible membrane protein; contains potential membrane spanning regions. Also contains PS00017 ATP/GTP-binding site motif A (P-loop). I6X9F4 No cell wall and cell processes I6X9F4 I6X9F4 NC_000962.3 Mycobrowser_v5 CDS 716410 716664 . + 0 Rv0623 vapB30 Unknown Possible antitoxin VapB30 Rv0623, (MTCY20H10.04), len: 84 aa. Possible vapB30, antitoxin, part of toxin-antitoxin (TA) operon with Rv0624, see Arcus et al. 2005. Also similar to others in Mycobacterium tuberculosis e.g MTCY28_2|Rv1740|MTCY28.02|MTCY04C12.25 conserved hypothetical protein (70 aa), FASTA score: (73.5% identity in 68 aa overlap); MTCY4C12_25|Rv0608|MTCY19H5.14c conserved hypothetical protein (81 aa), FASTA score: (73.5 identity in 68 aa overlap); etc. P9WJ35 No virulence, detoxification, adaptation P96913 P9WJ35 P9WJ35 NC_000962.3 Mycobrowser_v5 CDS 716664 717059 . + 0 Rv0624 vapC30 Unknown Possible toxin VapC30. Contains PIN domain. Rv0624, (MTCY20H10.05), len: 131 aa. Possible vapC30, toxin, part of toxin-antitoxin (TA) operon with Rv0623, contains PIN domain, see Arcus et al. 2005. Highly similar to others in Mycobacterium tuberculosis e.g. Rv1741, Rv0609, Rv2759c,Rv0565c, Rv3854c, Rv3083, etc. P9WF77 No virulence, detoxification, adaptation P67240 P9WF77 P9WF77 NC_000962.3 Mycobrowser_v5 CDS 717153 717893 . - 0 Rv0625c Rv0625c Unknown Probable conserved transmembrane protein Rv0625c, (MTCY20H10.06c), len: 246 aa. Probable conserved transmembrane protein, showing similarity with others e.g. CAB61866.1|AL133252 putative integral membrane protein from Streptomyces coelicolor (249 aa). Also similar to Rv1491c|MTCY277_13 from Mycobacterium tuberculosis. Contains potential membrane spanning regions. P9WFS5 No cell wall and cell processes P67115 P9WFS5 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 718761 719912 . - 0 Rv0628c Rv0628c Function unknown Conserved hypothetical protein Rv0628c, (MTCY20H10.09c), len: 383 aa. Conserved hypothetical protein, highly similar to Rv0874c|YZ02_MYCTU|Q10536 conserved hypothetical protein from Mycobacterium tuberculosis (386 aa), FASTA scores: opt: 2082, E(): 0, (81.5% identity in 383 aa overlap). Also some similarity to P72543|SPU62616_1 hypothetical protein from Synechococcus, FASTA scores: E(): 2.8e-28, (36.6 identity in 265 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WKS7 No conserved hypotheticals P64729 P9WKS7 GO:0005886,GO:0016021 P9WKS7 NC_000962.3 Mycobrowser_v5 CDS 732393 732821 . + 0 Rv0636 hadB Involved in fatty acid synthesis type II (fas-II) (3R)-hydroxyacyl-ACP dehydratase subunit HadB Rv0636, (MTCY20H10.17), len: 142 aa. HadB, (3R)-hydroxyacyl-ACP dehydratase subunit, equivalent to NP_302286.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (142 aa). Shows structural similarity to six others in Mycobacterium tuberculosis (see Castell et al (2005) below). Also highly similar to CAB77411.1|AL160431|SCD82.08 hypothetical protein from Streptomyces coelicolor (142 aa); and similar to others e.g. U28943|CELE04F6_3 from Caenorhabditis elegans (cosmid E04) (298 aa), FASTA scores: opt: 167, E(): 0.00064, (31.6 identity in 117 aa overlap). I6WYY7 No intermediary metabolism and respiration P96927 I6WYY7 GO:0016491,GO:0008152 I6WYY7 NC_000962.3 Mycobrowser_v5 CDS 735022 735450 . + 0 Rv0640 rplK This protein binds directly to 23S ribosomal RNA. 50S ribosomal protein L11 RplK Rv0640, (MTCY20H11.21), len: 142 aa. rplK, 50S ribosomal protein L11, equivalent to NP_302282.1|NC_002677 50S ribosomal protein L11 from Mycobacterium leprae (142 aa). Also highly similar to others e.g. P48954|RL11_STRCO|SCD82.19 50s ribosomal protein L11 from Streptomyces coelicolor (144 aa), FASTA scores: opt: 763, E(): 0, (84.6% identity in 143 aa overlap); etc. Contains PS00359 Ribosomal protein L11 signature. Belongs to the L11P family of ribosomal proteins. P9WHE5 No information pathways P66056 P9WHE5 GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WHE5 NC_000962.3 Mycobrowser_v5 CDS 721729 725013 . - 0 Rv0630c recB Involved in homologous recombination. Probable exonuclease V (beta chain) RecB (exodeoxyribonuclease V beta chain)(exodeoxyribonuclease V polypeptide) (chi-specific endonuclease) Rv0630c, (MTCY20H10.11c), len: 1094 aa. Probable recB, exonuclease V, beta chain (exodeoxyribonuclease V, beta chain) (see citation below), highly similar to other exonucleases e.g. AF157643_2|recB|AAD46808.1 Escherichia coli RecB protein homolog from Mycobacterium smegmatis (1083 aa); P08394|EX5B_ECOLI|RORA|B2820 exodeoxyribonuclease V 135 kDa polypeptide (exonuclease V beta chain) from Escherichia coli strain K12 (1180 aa), FASTA scores: opt: 289, E(): 4.3e-11, (29.5 identity in 1059 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the helicase family, UVRD subfamily. Consists of three subunits; RECB, RECC|Rv0631c and recd|Rv0629c. P9WMQ3 No information pathways P96920 P9WMQ3 GO:0004003,GO:0003677,GO:0006281,GO:0004519,GO:0008854,GO:0005524 3.1.11.5 P9WMQ3 NC_000962.3 Mycobrowser_v5 CDS 731930 732406 . + 0 Rv0635 hadA Involved in fatty acid synthesis type II (fas-II) (3R)-hydroxyacyl-ACP dehydratase subunit HadA Rv0635, (MTCY20H10.16), len: 158 aa. HadA, (3R)-hydroxyacyl-ACP dehydratase subunit, equivalent to NP_302287.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (159 aa); and highly similar to YV31_MYCLE|P54879 conserved hypothetical protein from Mycobacterium leprae (166 aa), FASTA scores: opt: 387, E(): 5.9e-21, (43.4% identity in 145 aa overlap). Also similar CAB77410.1|AL160431|SCD82.07 hypothetical protein from Streptomyces coelicolor (150 aa). And highly similar to two hypothetical proteins from Mycobacterium tuberculosis: Rv0504c|YV31_MYCTU|Q11168 (166 aa), FASTA scores: opt: 405, E(): 3.2e-22, (45.0% identity in 140 aa overlap); and Rv0637|MTY20H10_19 (2 ORFs downstream) (166 aa), FASTA score: (48.7% identity in 150 aa overlap). P9WFK1 No intermediary metabolism and respiration P9WFK1 P9WFK1 NC_000962.3 Mycobrowser_v5 CDS 746363 747037 . + 0 Rv0649 fabD2 Involved in lipid metabolism; fatty acid biosynthesis [catalytic activity: malonyl-CoA + [acyl-carrier protein] = CoA + malonyl-[acyl-carrier protein]]. Possible malonyl CoA-acyl carrier protein transacylase FabD2 (MCT) Rv0649, (MTCY20H10.30), len: 224 aa. Possible fabD2, malonyl CoA-acyl carrier protein transacylase, similar to mtfabd|FABD_MYCTU|Q10501|Rv2243 malonyl CoA-acyl carrier protein transacylase from Mycobacterium tuberculosis (302 aa), FASTA scores: opt: 133, E(): 0.074, (31.3% identity in 147 aa overlap). Q79FX5 No lipid metabolism Q79FX5 GO:0004314 2.3.1.39 Q79FX5 NC_000962.3 Mycobrowser_v5 CDS 754685 754993 . - 0 Rv0659c mazF2 Function unknown Toxin MazF2 Rv0659c, (MTCI376.17), len: 102 aa. MazF2, toxin, part of toxin-antitoxin (TA) operon with Rv0660c (See Pandey and Gerdes, 2005; Zhu et al., 2006), weakly similar to other Mycobacterium tuberculosis hypothetical proteins e.g. Rv1942c, Rv1495, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WII1 No virulence, detoxification, adaptation O06780 P9WII1 GO:0003677 P9WII1 NC_000962.3 Mycobrowser_v5 CDS 1301307 1301747 . + 0 Rv1171 Rv1171 Unknown Conserved hypothetical protein Rv1171, (MTV005.07), len: 146 aa. Conserved hypothetical protein, possibly transmembrane protein. Start has been changed since first submission. O50427 No conserved hypotheticals O50427 NC_000962.3 Mycobrowser_v5 tRNA 731494 731566 . + 0 MTB000006 thrT - tRNA-Thr (GGT) thrT, tRNA-Thr, anticodon ggt, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 731603 731676 . + 0 MTB000007 metT - tRNA-Met (CAT) metT, tRNA-Met, anticodon cat, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 3128973 3129347 . - 0 Rv2822c Rv2822c Unknown Hypothetical protein Rv2822c, (MTCY16B7.20), len: 124 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WJG1 No conserved hypotheticals P71630 P9WJG1 P9WJG1 NC_000962.3 Mycobrowser_v5 CDS 733737 734222 . + 0 Rv0638 secE1 Essential for protein export. Probable preprotein translocase SecE1 Rv0638, (MTCY20H10.19), len: 161 aa. Probable secE1, preprotein translocase (tail-anchored membrane protein) (see citation below), highly similar at C-terminal half to others e.g. P36690|SECE_STRGR preprotein translocase SECE subunit from Streptomyces griseus (86 aa), FASTA scores: opt: 220, E(): 4.6e-06, (35.4% identity in 96 aa overlap); P16920|SECE_ECOLI preprotein translocase sece subunit from Escherichia coli strains K12 and O157:H7 (127 aa), FASTA scores: opt: 122, E(): 0.34, (37.0% identity in 54 aa overlap); etc. Contains PS01067 Protein secE/sec61-gamma signature. Belongs to the SECE/SEC61-gamma family. Part of the prokaryotic protein translocation apparatus which comprise SECA|Rv3240c, SECD|Rv2587c, SECE, SECF|Rv2586c, SECG|Rv1440 and SECY|Rv0732. Note that previously known as secE. P9WGN7 No cell wall and cell processes P0A5Z0 P9WGN7 GO:0016021,GO:0005886,GO:0009306,GO:0006605,GO:0055085,GO:0015450 P9WGN7 NC_000962.3 Mycobrowser_v5 CDS 734254 734970 . + 0 Rv0639 nusG Influences transcription termination and antitermination. Acts as a component of the transcription complex, and interacts with the termination factor rho and RNA polymerase. Probable transcription antitermination protein NusG Rv0639, (MTCY20H10.20), len: 238 aa. Probable nusG, transcription antitermination protein, equivalent to NP_302283.1|NC_002677 transcription antitermination protein nusG from Mycobacterium leprae (228 aa). Also highly similar to others e.g. P36260|NUSG_STRGR from Streptomyces griseus (294 aa), FASTA scores: opt: 845, E(): 0, (55.4% identity in 233 aa overlap); etc. Note that shorter at the N-terminus than other nusG. Contains PS01014 Transcription termination factor nusG signature. Belongs to the NusG family. P9WIU9 No information pathways P65589 P9WIU9 GO:0031564,GO:0003711,GO:0006353,GO:0032968 P9WIU9 NC_000962.3 Mycobrowser_v5 CDS 2545737 2546105 . + 0 Rv2272 Rv2272 Unknown Probable conserved transmembrane protein Rv2272, (MTCY339.38c), len: 122 aa. Probable conserved transmembrane protein, similar to YIDH_ECOLI P31445 hypothetical 12.8 kDa protein (115 aa), FASTA scores, opt: 291, E(): 2.9e-14, (45.6% identity in 103 aa overlap), similar to MTCY339.37c, (35.0% identity in 100 aa overlap). P9WLF5 No cell wall and cell processes P64969 P9WLF5 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 737268 738149 . - 0 Rv0643c mmaA3 Involved in mycolic acids modification. Catalyzes unusual S-adenosyl-methionine-dependent transformation of a cis-olefin mycolic acid into a secondary alcohol. Catalyzes introduction of a hydroxyl group at the distal position on mycolic acid chains to produce the hydroxyl mycolate. Mycolic acids represent a major constituent of the mycobacterial cell wall complex. Methyl transfer results in formation of a secondary hydroxy group with an adjacent methyl branch; olefinic mycolic acid methyl transferase. Methoxy mycolic acid synthase 3 MmaA3 (methyl mycolic acid synthase 3) (MMA3) (hydroxy mycolic acid synthase) Rv0643c, (MTCY20H10.24c), len: 293 aa. MmaA3, methoxy mycolic acid synthase 3 (methyltransferase) (see citations below). Equivalent to AAC44875|AAC44875.1|cmaB methyl transferase (mycolic acid modification protein) from Mycobacterium bovis BCG strain Pasteur (289 aa); and highly similar to others from Mycobacteria e.g. upstream ORF P72028|mmaA4|Rv0642c|MTCY20H10.23c putative methoxy mycolic acid synthase 4 from Mycobacterium tuberculosis (301 aa). P0CH91 No lipid metabolism P72027 P0CH91 GO:0008610,GO:0008825 2.1.1.- P0CH91 NC_000962.3 Mycobrowser_v5 CDS 738297 739160 . - 0 Rv0644c mmaA2 Involved in mycolic acids modification. Catalyzes unusual S-adenosyl-methionine-dependent transformation of a cis-olefin mycolic acid into a secondary alcohol. Catalyzes introduction of a hydroxyl group at the distal position on mycolic acid chains to produce the hydroxyl mycolate. Mycolic acids represent a major constituent of the mycobacterial cell wall complex. Methyl transfer results in formation of a secondary hydroxy group with an adjacent methyl branch; olefinic mycolic acid methyl transferase. Has also cyclopropane function. Methoxy mycolic acid synthase 2 MmaA2 (methyl mycolic acid synthase 2) (MMA2) (hydroxy mycolic acid synthase) Rv0644c, (MTCY20H10.25c), len: 287 aa. MmaA2, methoxy mycolic acid synthase 2 (methyltransferase) (see citations below). Equivalent to AAC44874|AAC44874.1|cmaC methyl transferase (mycolic acid modification protein) from Mycobacterium bovis BCG strain Pasteur (287 aa); and highly similar to others from Mycobacteria e.g. upstream ORF P72028|mmaA4|Rv0642c|MTCY20H10.23c putative methoxy mycolic acid synthase 4 from Mycobacterium tuberculosis (301 aa). Note that alternative start is at position 739247. Q79FX6 No lipid metabolism 1TPY Q79FX6 Q79FX6 GO:0008610,GO:0008825 2.1.1.- Q79FX6 NC_000962.3 Mycobrowser_v5 CDS 741151 742617 . - 0 Rv0647c Rv0647c Function unknown Conserved protein Rv0647c, (MTCY20H10.28c), len: 488 aa. Conserved protein, equivalent to NP_302277.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (448 aa). Also showing similarity to a variety of hypothetical ABC1-like proteins or conserved hypothetical proteins e.g. D90908_28|P73627 ABC1-like protein from Synechocystis (585 aa), FASTA scores: E(): 1.8e-31, (29.1% identity in 474 aa overlap); Q55884 HYPOTHETICAL6 5.0 KD protein (567 aa), FASTA scores: opt: 583, E(): 5.7e-30, (28.1% identity in 416 aa overlap); etc. Also similar to Rv3197 conserved hypothetical protein from Mycobacterium tuberculosis. P9WQI1 No conserved hypotheticals P96936 P9WQI1 GO:0006468,GO:0004672,GO:0005524 P9WQI1 NC_000962.3 Mycobrowser_v5 CDS 800487 800792 . + 0 Rv0700 rpsJ This protein is involved in the binding of tRNA to the ribosomes, and in the regulation of rRNA biosynthesis (by modulating the efficiency of transcriptional termination). Interacts with NUSB|Rv2533c. 30S ribosomal protein S10 RpsJ (transcription antitermination factor NusE) Rv0700, (MTCY210.19), len: 101 aa. rpsJ (alternate gene name: nusE), 30S ribosomal protein S10 (see Gopal et al., 2001), equivalent to RS10_MYCLE P307653 30S ribosomal protein S10 from Mycobacterium leprae (101 aa), FASTA scores: opt: 645, E(): 0, (97.0% identity in 101 aa overlap). Also highly similar to others e.g. CAB82069.1|AL161803 30S ribosomal protein S10 from Streptomyces coelicolor (102 aa); etc. Contains PS00361 Ribosomal protein S10 signature. Belongs to the S10P family of ribosomal proteins. P9WH67 No information pathways P0A5X0 P9WH67 GO:0003735,GO:0000049,GO:0006412,GO:0015935 P9WH67 NC_000962.3 Mycobrowser_v5 CDS 740234 741139 . - 0 Rv0646c lipG Function unknown; lipolytic enzyme involved in cellular metabolism. Probable lipase/esterase LipG Rv0646c, (MTCY20H10.27c), len: 301 aa. Probable lipG, lipase/esterase, equivalent to NP_302278.1|NC_002677 probable hydrolase from Mycobacterium leprae (304 aa). Also highly similar to various hydrolases, especially lipases e.g. AA61351.1|X88895 carboxyl esterase from Acinetobacter calcoaceticus (312 aa), FASTA scores: opt: 867, E(): 0, (50.2% identity in 279 aa overlap); etc. Also similar to transferases e.g. P77026 macrolide 2'-phosphotransferase II from Escherichia coli (279 aa), FASTA scores: E(): 1.3e-14, (32.5% identity in 286 aa overlap). Similar to M. tuberculosis non-heme bromoperoxidases and epoxide hydrolases. P96935 No intermediary metabolism and respiration P96935 P96935 GO:0016787 3.1.-.- P96935 NC_000962.3 Mycobrowser_v5 CDS 747037 747945 . + 0 Rv0650 Rv0650 Function unknown; probably involved in specific sugar metabolism or regulation. Possible sugar kinase Rv0650, (MTCY20H10.31), len: 302 aa. Possible sugar kinase, highly similar to others e.g. CAB95296.1|AL359779 putative sugar kinase from Streptomyces coelicolor (317 aa); NP_406512.1|NC_003143 putative sugar kinase from Yersinia pestis (290 aa); NP_229269.1|NC_000853 glucokinase from Thermotoga maritima (317 aa); etc.Contains PS01125 ROK family signature. Belongs to the ROK (NAGC/XYLR) family. I6Y8D3 No intermediary metabolism and respiration P96939 I6Y8D3 GO:0016301 2.7.-.- I6Y8D3 NC_000962.3 Mycobrowser_v5 CDS 748276 748812 . + 0 Rv0651 rplJ Involved in translation mechanisms. 50S ribosomal protein L10 RplJ Rv0651, (MTCY20H10.32), len: 178 aa. rplJ, 50S ribosomal protein L10, equivalent to NP_302276.1|NC_002677 50S ribosomal protein L10 from Mycobacterium leprae (177 aa). Also highly similar to others e.g. P36257|RL10_STRGR 50s ribosomal protein L10 from Streptomyces griseus (185 aa), FASTA scores: opt: 633, E(): 0, (59.0 % identity in 173 aa overlap); etc. Belongs to the L10P family of ribosomal proteins. P9WHE7 No information pathways P66044 P9WHE7 GO:0042254,GO:0003735,GO:0006412,GO:0005840 P9WHE7 NC_000962.3 Mycobrowser_v5 CDS 791658 791846 . + 0 Rv0691A Rv0691A Electron carrier Mycofactocin precursor protein Rv0691A, len: 62 aa. Mycofactocin precursor protein. P9WJ81 No intermediary metabolism and respiration P9WJ81 NC_000962.3 Mycobrowser_v5 CDS 809946 810887 . + 0 Rv0713 Rv0713 Unknown Probable conserved transmembrane protein Rv0713, (MTCY210.32), len: 313 aa. Probable conserved transmembrane protein, similar to Rv3435c|MTCY77_7|O06252 from Mycobacterium tuberculosis (284 aa), FASTA scores: opt: 557, E(): 2.1e-29, (35.8% identity in 282 aa overlap); MLCB2492_12|O32991 hypothetical 10.7 kDa protein from Mycobacterium leprae (95 aa). I6WZ58 No cell wall and cell processes I6WZ58 GO:0016021 I6WZ58 NC_000962.3 Mycobrowser_v5 CDS 1662381 1662572 . + 0 Rv1473A Rv1473A Possibly involved in transcriptional mechanism Possible transcriptional regulatory protein Rv1473A, len: 63 aa. Possible transcriptional regulator, CDS predicted by GC plot. Similar to SCI8.24c|AL132644_24 putative transcriptional regulator from Streptomyces coelicolor (73 aa), FASTA scores: opt: 210, E(): 1.5e-08, (56.15% identity in 57 aa overlap). L7N691 No regulatory proteins L7N691 NC_000962.3 Mycobrowser_v5 CDS 748849 749241 . + 0 Rv0652 rplL Involved in translation mechanisms: seems to be the binding site for several of the factors involved in protein synthesis and appears to be essential for accurate translation. 50S ribosomal protein L7/L12 RplL (SA1) Rv0652, (MTCY20H10.33), len: 130 aa. rplL (alternate gene name: L7|L12), 50S ribosomal protein L7/L12, equivalent to NP_302275.1|NC_002677 50S ribosomal protein L7/L12 from Mycobacterium leprae (130 aa); and P37381|RL7_MYCBO 50s ribosomal protein L7/L12 from Mycobacterium bovis (130 aa). Also highly similar to others e.g. P02396|RL7_STRGR 50S ribosomal protein L7/L12 from Streptomyces griseus (127 aa); etc. Belongs to the L12P family of ribosomal proteins. P9WHE3 No information pathways P0A5V2 P9WHE3 GO:0003735,GO:0006412,GO:0005840 P9WHE3 NC_000962.3 Mycobrowser_v5 CDS 759807 763325 . + 0 Rv0667 rpoB Catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [catalytic activity: N nucleoside triphosphate = N diphosphate + {RNA}(N)]. DNA-directed RNA polymerase (beta chain) RpoB (transcriptase beta chain) (RNA polymerase beta subunit) Rv0667, (MTCI376.08c), len: 1172 aa. RpoB, DNA-directed RNA polymerase, beta chain (see Miller et al., 1994; Ahmad et al., 2000), equivalent to P30760|RPOB_MYCLE|ML1891 DNA-directed RNA polymerase beta chain from Mycobacterium leprae (1178 aa). Also highly similar to others e.g. AAF60349.1|AF242549_1|AF242549 DNA-dependent RNA polymerase beta subunit from Amycolatopsis mediterranei (1167 aa); CAB77428.1|AL160431 DNA-directed RNA polymerase beta chain from Streptomyces coelicolor (1161 aa); etc. Start site chosen on basis of RBS but alternative start exists at position 14359. Belongs to the RNA polymerase beta chain family. P9WGY9 No information pathways P0A680 P9WGY9 GO:0003899,GO:0046677,GO:0032549,GO:0006351,GO:0003677 2.7.7.6 RIF P9WGY9 NC_000962.3 Mycobrowser_v5 CDS 751517 752596 . + 0 Rv0655 mkl Thought to be involved in active transport of ribonucleotide across the membrane. Responsible for energy coupling to the transport system. Possible ribonucleotide-transport ATP-binding protein ABC transporter Mkl Rv0655, (MTCI376.21), len: 359 aa. Possible mkl, ribonucleotide-transport ATP-binding protein ABC transporter (see Braibant et al., 2000), equivalent to P30769|MKL_MYCLE|ML1892 possible ribonucleotide transport ATP-binding protein from Mycobacterium leprae (347 aa), FASTA scores: opt: 2021, E(): 0, (92.2% identity in 335 aa overlap). Also highly similar to many e.g. AB92896.1|AL356992 putative ABC-transporter ATP-binding protein from Streptomyces coelicolor (343 aa); NP_253146.1|NC_002516 probable ATP-binding component of ABC transporter from Pseudomonas aeruginosa (269 aa); P45393|YRBF_ECOLI hypothetical ABC transporter ATP-binding protein from Escherichia coli (269 aa), FASTA scores: opt: 644, E(): 3.4e-33, (38.5% identity in 244 aa overlap); etc. Also similar to many other Mycobacterium tuberculosis ABC transporters e.g. P71747|CYSA|Rv2397c|MTCY253.24 (351 aa), FASTA score: (33.6% identity in 241 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). P9WQL5 No cell wall and cell processes P63357 P9WQL5 GO:0016887,GO:0006810,GO:0005524 P9WQL5 NC_000962.3 Mycobrowser_v5 CDS 752984 753367 . - 0 Rv0656c vapC6 Unknown Possible toxin VapC6 Rv0656c, (MTCI376.20), len: 127 aa. Possible vapC6, toxin, part of toxin-antitoxin (TA) operon with Rv0657c, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to other proteins from Mycobacterium tuberculosis e.g. Rv2757c, Rv2546, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WFB5 No virulence, detoxification, adaptation O06783 P9WFB5 P9WFB5 NC_000962.3 Mycobrowser_v5 CDS 753462 753617 . - 0 Rv0657c vapB6 Unknown Possible antitoxin VapB6 Rv0657c, (MTCI376.19), len: 51 aa. Possible vapB6, antitoxin, part of toxin-antitoxin (TA) operon with Rv0656c (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similarity with others from Mycobacterium tuberculosis e.g. Rv2009|MT2064.1|MTCY39.08c|YW08_MYCTU|Q10848 (80 aa), FASTA scores: opt: 107, E(): 0.0038, (45.8% identity in 48 aa overlap), Rv2871, Rv1560, etc. Also some similarity with AL020958|SC4H8_7 from Streptomyces coelicolor (66 aa), FASTA score: (41.0% identity in 39 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WJ57 No virulence, detoxification, adaptation O06782 P9WJ57 NC_000962.3 Mycobrowser_v5 CDS 798833 799444 . + 0 Rv0698 Rv0698 Function unknown Conserved hypothetical protein Rv0698, (MTCY210.15), len: 203 aa. Conserved hypothetical protein, highly similar to C-terminus of Rv3639c|MTY15C10.12 conserved hypothetical protein from Mycobacterium tuberculosis (188 aa), FASTA scores: E(): 2.1e-07, (54.8% identity in 73 aa overlap). P95044 No conserved hypotheticals P95044 NC_000962.3 Mycobrowser_v5 CDS 881075 881464 . - 0 Rv0786c Rv0786c Function unknown Conserved protein Rv0786c, (MTCY369.30c), len: 129 aa. Conserved protein, similar to three other hypothetical proteins from Streptomyces coelicolor e.g. SC7H1.08c|T35703 hypothetical protein (202 aa), FASTA scores: opt: 241, E(): 5.1e-10, (41.0% identity in 105 aa overlap); SC3A7.08|T29426 (211 aa). P71839 No conserved hypotheticals P71839 P71839 NC_000962.3 Mycobrowser_v5 CDS 755769 756023 . - 0 Rv0662c vapB7 Unknown Possible antitoxin VapB7 Rv0662c, (MTCI376.14), len: 84 aa. Possible vapB7, antitoxin, part of toxin-antitoxin (TA) operon with Rv0661c (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similarity with others from Mycobacterium tuberculosis e.g. Rv2871, Rv1241, Rv2550c, etc. Start changed since first submission, now 38 aa shorter. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06777 No virulence, detoxification, adaptation O06777 NC_000962.3 Mycobrowser_v5 CDS 759136 759309 . + 0 Rv0666 Rv0666 Unknown Possible membrane protein Rv0666, (MTCI376.10c), len: 57 aa. Possible membrane protein; has hydrophobic stretch at aa 29-47. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06773 No cell wall and cell processes O06773 NC_000962.3 Mycobrowser_v5 CDS 755335 755772 . - 0 Rv0661c vapC7 Unknown Possible toxin VapC7 Rv0661c, (MTCI376.15), len: 145 aa. Possible vapC7, toxin, part of toxin-antitoxin (TA) operon with Rv0662c, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. Rv2863|MTV003.09|MTV003_7 (126 aa), FASTA scores: E(): 0.00087, (30.4% identity in 125 aa overlap), Rv0749|MTV041.23 (163 aa); Rv0277c, Rv2530c, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WFB3 No virulence, detoxification, adaptation O06778 P9WFB3 P9WFB3 NC_000962.3 Mycobrowser_v5 CDS 758532 758804 . + 0 Rv0664 vapB8 Unknown Possible antitoxin VapB8 Rv0664, (MTCI376.12c), len: 90 aa. Possible vapB8, antitoxin, part of toxin-antitoxin (TA) operon with Rv0665 (See Arcus et al., 2005; Pandey and Gerdes, 2005). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06775 No virulence, detoxification, adaptation O06775 O06775 NC_000962.3 Mycobrowser_v5 CDS 758801 759139 . + 0 Rv0665 vapC8 Unknown Possible toxin VapC8 Rv0665, (MTCI376.11c), len: 112 aa. Possible vapC8, toxin, part of toxin-antitoxin (TA) operon with Rv0664, contains PIN domain (See Arcus et al. 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. Rv0627 (135 aa), and Rv0595c. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WFB1 No virulence, detoxification, adaptation O06774 P9WFB1 P9WFB1 NC_000962.3 Mycobrowser_v5 CDS 769792 770550 . + 0 Rv0670 end Involved in base excision repair. Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5' ends that are base-free deoxyribose 5-phosphate residues [catalytic activity: endonucleolytic cleavage to 5'-phosphooligonucleotide end-products]. Probable endonuclease IV End (endodeoxyribonuclease IV) (apurinase) Rv0670, (MTCI376.04c), len: 252 aa. Probable end (alternate gene name: nfo), endonuclease IV (apurinase) (see citation below), equivalent to END_MYCLE|P30770|NFO|ML1889 probable endonuclease IV (apurinase) from Mycobacterium leprae (252 aa), FASTA scores: opt: 1463, E(): 0, (85.6% identity in 250 aa overlap). Also similar to others e.g. Q9S2N2|END4_STRCO|NFO|SC6E10.05 probable endonuclease IV from Streptomyces coelicolor (294 aa); etc. Contains PS00729 AP endonucleases family 2 signatures 1 and 2 (PS00729, and PS00730). Belongs to the AP endonucleases family 2. Cofactor: binds 3 zinc ions. The transcription of this CDS seems negatively regulated by the product of mce2R|Rv0586 (See Santangelo et al., 2009). P9WQ13 No information pathways P63535 P9WQ13 GO:0006281,GO:0008833,GO:0005622,GO:0008270,GO:0003677 3.1.21.2 P9WQ13 NC_000962.3 Mycobrowser_v5 CDS 778477 778905 . - 0 Rv0677c mmpS5 Unknown Possible conserved membrane protein MmpS5 Rv0677c, (MTV040.05c), len: 142 aa. Possible mmpS5, conserved membrane protein (see Tekaia et al., 1999), highly similar to other Mycobacterial proteins e.g. P54880|MMS4_MYCLE putative membrane protein from Mycobacterium leprae (154 aa), FASTA scores: opt: 443, E(): 1.4e-23, (47.1% identity in 155 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis. Belongs to the MmpS family. Predicted to be an outer membrane protein (See Song et al., 2008). P9WJS7 No cell wall and cell processes P65380 P9WJS7 GO:0016021 P9WJS7 NC_000962.3 Mycobrowser_v5 CDS 811373 811741 . + 0 Rv0714 rplN This protein binds directly to 23S ribosomal RNA. 50S ribosomal protein L14 RplN Rv0714, (MTCY210.33), len: 122 aa. rplN, 50S ribosomal protein L14, equivalent to O32993|MLCB2492_14|ML1849|RL14_MYCLE 50S ribosomal protein L14 from Mycobacterium leprae (122 aa). Also highly similar to others e.g. CAB82080.1|AL161803 50S ribosomal protein L14 from Streptomyces coelicolor (122 aa); P33100|RL14_MICLU 50s ribosomal protein L14 from Micrococcus luteus (122 aa), FASTA scores: opt: 674, E(): 0, (85.2% identity in 122 aa overlap); etc. Contains PS00049 Ribosomal protein L14 signature. Belongs to the L14P family of ribosomal proteins. P9WHD9 No information pathways P66069 P9WHD9 GO:0019843,GO:0003735,GO:0006412,GO:0015934 P9WHD9 NC_000962.3 Mycobrowser_v5 CDS 2348558 2349292 . - 0 Rv2091c Rv2091c Unknown Probable membrane protein Rv2091c, (MTCY49.31c), len: 244 aa. Probable membrane protein; contains potential transmembrane region. Repetitive ORF. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WLJ5 No cell wall and cell processes P9WLJ5 GO:0016021 P9WLJ5 NC_000962.3 Mycobrowser_v5 CDS 773123 774061 . + 0 Rv0673 echA4 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Possible enoyl-CoA hydratase EchA4 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv0673, (MTCI376.01c, MTV040.01), len: 312 aa. Possible echA4, enoyl-CoA hydratase, showing similarity with others e.g. NP_419216.1|NC_002696 enoyl-CoA hydratase/isomerase family protein from Caulobacter crescentus (256 aa); Q52995|ECHH_RHIME probable enoyl-CoA hydratase from Sinorhizobium meliloti (257 aa), FASTA scores: opt: 210, E(): 1.2e-06, (27.9% identity in 280 aa overlap); etc. Also similar to other enoyl-CoA hydratases from Mycobacterium tuberculosis e.g. P95279|MTCY09F9.29|ECHA13|Rv1935c|MTCY09F9.29 enoyl-CoA hydratase (318 aa), FASTA score: (27.1% identity in 280 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). I6Y8F2 No lipid metabolism O86369 I6Y8F2 GO:0016853,GO:0008152,GO:0004300 4.2.1.17 I6Y8F2 NC_000962.3 Mycobrowser_v5 CDS 774064 774786 . + 0 Rv0674 Rv0674 Function unknown Conserved hypothetical protein Rv0674, (MTV040.02), len: 240 aa. Conserved hypothetical protein, highly similar to AC13063.1|AL445503 conserved hypothetical protein from Streptomyces coelicolor (268 aa); and similar to NP_438100.1|NC_003078 putative regulator of phenylacetic acid degradation ArsR family protein from Sinorhizobium meliloti (306 aa) and other proteins e.g. AB011837|AB011837_13 hypothetical protein from Bacillus halodurans (298 aa), FASTA scores: opt: 148, E(): 0.0081, (25.1% identity in 235 aa overlap); etc. I6WZ26 No conserved hypotheticals 3KFW O53782 I6WZ26 I6WZ26 NC_000962.3 Mycobrowser_v5 CDS 775586 778480 . - 0 Rv0676c mmpL5 Unknown. Thought to be involved in fatty acid transport. Probable conserved transmembrane transport protein MmpL5 Rv0676c, (MTV040.04c), len: 964 aa. Probable mmpL5, conserved transmembrane transport protein (see Tekaia et al., 1999), member of RND superfamily, highly similar to other Mycobacterial proteins e.g. MTV037_14, MTCY98_8, MTCY20G9_34, MTCY4D9_15, MTCY48_8, MTCY19G5_6, MTV005_19, etc. Also similar to other Mycobacterial mmpl proteins e.g. P54881|MML4_MYCLE putative membrane protein MMPL4 from Mycobacterium leprae (959 aa), FASTA scores: opt: 3991, E(): 0, (62.8% identity in 933 aa overlap); etc. Belongs to the MmpL family. P9WJV1 No cell wall and cell processes O53784 P9WJV1 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 780042 780416 . - 0 Rv0680c Rv0680c Unknown Probable conserved transmembrane protein Rv0680c, (MTV040.08c), len: 124 aa. Possible conserved transmembrane protein, showing similarity with C-terminal part of Rv0314c|Z96800|MTCY63.19c conserved hypothetical protein from Mycobacterium tuberculosis (220 aa), FASTA scores: opt: 175, E(): 2.2e-05, (31.4% identity in 102 aa overlap). Also some similarity to upstream ORF Rv0679c|MTV040.07c conserved hypothetical threonine rich protein (124 aa), FASTA score: (35.1% identity in 131 aa overlap). Contains possible membrane spanning regions. I6Y4F1 No cell wall and cell processes O53788 I6Y4F1 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 780721 781311 . + 0 Rv0681 Rv0681 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (possibly TetR-family) Rv0681, (MTV040.09), len: 196 aa. Probable transcription regulator, TetR family, similar to others and especially many tetracycline repressors e.g. T34657 probable transcription regulator from Streptomyces coelicolor (189 aa); AF0278|AF027868_40|NP_389788.1|NC_000964 yobS regulator from Bacillus subtilis (191 aa), FASTA scores: opt: 213, E(): 1.6e-07, (28.8% identity in 153 aa overlap); P09164|TER4_ECOLI tetracycline repressor protein from Escherichia coli (217 aa), FASTA scores: opt: 145, E(): 0.0068, (39.0% identity in 59 aa overlap); etc. Contains helix-turn-helix motif at aa 28-49 (Score 1020, +2.66 SD). O53789 No regulatory proteins O53789 O53789 GO:0006350,GO:0003700,GO:0006355 O53789 NC_000962.3 Mycobrowser_v5 CDS 781934 782404 . + 0 Rv0683 rpsG Protein S7 binds specifically to part of the 3' end of 16S ribosomal RNA. 30S ribosomal protein S7 RpsG Rv0683, (MTV040.11), len: 156 aa. rpsG, 30S ribosomal protein S7 (see citation below), equivalent to others from Mycobacteria e.g. P41193|RS7_MYCSM 30S ribosomal protein S7 from Mycobacterium smegmatis (156 aa), FASTA scores: opt: 986, E(): 0, (96.2% identity in 156 aa overlap); Q53539|RS7_MYCBO 30S ribosomal protein S7 from Mycobacterium bovis (156 aa); etc. Also highly similar to others e.g. Q9L0K4|RS7_STRCO 30S ribosomal protein S7 from Streptomyces coelicolor (156 aa); etc. Contains PS00052 Ribosomal protein S7 signature. Belongs to the S7P family of ribosomal proteins. P9WH29 No information pathways P41194 P9WH29 GO:0015935,GO:0003735,GO:0000049,GO:0006412,GO:0019843 P9WH29 NC_000962.3 Mycobrowser_v5 CDS 782485 784590 . + 0 Rv0684 fusA1 This protein promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. Probable elongation factor G FusA1 (EF-G) Rv0684, (MTV040.12, MTCY210.01), len: 701 aa. Probable fusA1, elongation factor G, equivalent to P30767|EFG_MYCLE|S31150 translation elongation factor EF-G from Mycobacterium leprae (701 aa), FASTA scores: opt: 2521, E(): 0, (88.2% identity in 432 aa overlap). Also highly similar to others e.g. CAB81852.1|AL161691 elongation factor G from Streptomyces coelicolor (708 aa); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS00301 GTP-binding elongation factors signature. Belongs to the GTP-binding elongation factor family, EF-G/EF-2 subfamily. Note that previously known as fusA. P9WNM7 No information pathways P0A556 P9WNM7 GO:0003924,GO:0005737,GO:0003746,GO:0006414,GO:0005525 P9WNM7 NC_000962.3 Mycobrowser_v5 CDS 784821 786011 . + 0 Rv0685 tuf This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Probable iron-regulated elongation factor TU Tuf (EF-TU) Rv0685, (MTCY210.02), len: 396 aa. Probable tuf, iron-regulated elongation factor EF-Tu, equivalent to JC2262 translation elongation factor Tu from Mycobacterium leprae (396 aa). Also highly similar to others e.g. P42439|EFTU_CORGL elongation factor TU (EF-TU) from Corynebacterium glutamicum (396 aa); etc. Contains PS00017 ATP/GTP-binding site motif A, and PS00301 GTP-binding elongation factors signature. Belongs to the GTP-binding elongation factor family, EF-TU/EF-1A subfamily. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WNN1 No information pathways P0A558 P9WNN1 GO:0003924,GO:0005737,GO:0003746,GO:0006414,GO:0005525 P9WNN1 NC_000962.3 Mycobrowser_v5 CDS 790024 791073 . - 0 Rv0690c Rv0690c Function unknown Conserved hypothetical protein Rv0690c, (MTCY210.07c), len: 349 aa. Conserved hypothetical protein, showing similarity with NP_386956.1|NC_003047 conserved hypothetical protein from Sinorhizobium meliloti (358 aa); NP_356573.1|NC_003063 AGR_L_1570p from Agrobacterium tumefaciens (346 aa); NP_421938.1|NC_002696 conserved hypothetical protein from Caulobacter crescentus (370 aa). I6Y4G1 No conserved hypotheticals P95036 I6Y4G1 I6Y4G1 NC_000962.3 Mycobrowser_v5 CDS 792157 793332 . + 0 Rv0693 pqqE Required for coenzyme pyrrolo-quinoline-quinone (PQQ) biosynthesis. Probable coenzyme PQQ synthesis protein E PqqE (coenzyme PQQ synthesis protein III) Rv0693, (MTCY210.10), len: 391 aa. Probable pqqE (alternate gene name: pqqIII), coenzyme PQQ synthesis protein E, similar to others AE001109_9|O30258|PQQE coenzyme PQQ synthesis protein from Archaeoglobus fulgidus (375 aa), FASTA scores: E(): 1.6e-16, (28.1% identity in 377 aa overlap); PQQE_ACICA|P07782 coenzyme pqq synthesis protein e from Acinetobacter calcoaceticus (384 aa), FASTA scores: opt: 302, E(): 1.8e-12, (23.9% identity in 377 aa overlap); etc. Also similar to C-terminus of heme biosynthesis proteins e.g. O28270|AF2009 heme biosynthesis protein (NIRJ-2) from Archaeoglobus fulgidus (468 aa). Note that also highly similar to U17129|RSU17129_4|AAC77478.1 unknown protein from Rhodococcus erythropolis (405 aa), FASTA scores: opt: 1997, E(): 0, (73.3% identity in 390 aa overlap). Could belong to the MoaA / NifB / PqqE family. P9WJ79 No intermediary metabolism and respiration P95039 P9WJ79 GO:0051536,GO:0003824 P9WJ79 NC_000962.3 Mycobrowser_v5 CDS 794715 795470 . + 0 Rv0695 Rv0695 Function unknown Conserved hypothetical protein Rv0695, (MTCY210.12), len: 251 aa. Conserved hypothetical protein, similar to many creatinine amidohydrolases or hypothetical proteins e.g. NP_443048.1|NC_000911 creatinine amidohydrolase from Synechococcus sp. PCC 6803 (273 aa); NP_466169.1|NC_003210 protein similar to creatinine amidohydrolase from Listeria monocytogenes (249 aa); T35153|SC5A7.04c hypothetical protein from Streptomyces coelicolor (273 aa); etc. Note that highly similar to RSU17129_10|AAC77474.1|U17129 unknown protein from Rhodococcus erythropolis (230 aa), FASTA scores: opt: 693, E(): 0, (55.7% identity in 237 aa overlap). P9WP59 No conserved hypotheticals P95041 P9WP59 P9WP59 NC_000962.3 Mycobrowser_v5 CDS 796933 798372 . + 0 Rv0697 Rv0697 Function unknown; probably involved in cellular metabolism. Probable dehydrogenase Rv0697, (MTCY210.14, unknown), len: 479 aa. Probable dehydrogenase, highly similar to P30772|YTUR_MYCLE hypothetical 24 kDa protein from Mycobacterium leprae (220 aa), FASTA scores: opt: 557, E(): 1.7e-28, (46.2% identity in 223 aa overlap). Also highly similar to P46371|YTH2_RHOER hypothetical 53.0 KDA GMC-type oxidoreductase from Rhodococcus erythropolis (493 aa); and similar to many dehydrogenases e.g. NP_250814.1|NC_002516 probable dehydrogenase from Pseudomonas aeruginosa (545 aa); BAA13145.1|D86622 FAD dependent L-sorbose dehydrogenase from Gluconobacter oxydans (531 aa); etc. Also similar to Rv1279 conserved hypothetical protein from Mycobacterium tuberculosis. I6Y8H4 No intermediary metabolism and respiration P95043 I6Y8H4 GO:0006066,GO:0055114,GO:0016614,GO:0050660 1.-.-.- I6Y8H4 NC_000962.3 Mycobrowser_v5 CDS 800809 801462 . + 0 Rv0701 rplC This protein binds directly to 23S ribosomal RNA and may participate in the formation of the peptidyltransferase center of the ribosome. 50S ribosomal protein L3 RplC Rv0701, (MTCY210.20), len: 217 aa. rplC, 50S ribosomal protein L3, equivalent to O06044|RL3_MYCBO 50S ribosomal protein L3 from Mycobacterium bovis BCG (217 aa); and P30762|RL3_MYCLE 50S ribosomal protein L3 from Mycobacterium leprae (217 aa). Also highly similar to others e.g. CAB82070.1|AL161803 50S ribosomal protein L3 from Streptomyces coelicolor (214 aa); P52860|RL3_THETH ribosomal protein l3 from Thermus aquaticus (206 aa), FASTA scores: opt: 717, E(): 0, (55.2% identity in 210 aa overlap); etc. Contains PS00474 Ribosomal protein L3 signature. Belongs to the L3P family of ribosomal proteins. P9WH87 No information pathways P60442 P9WH87 GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WH87 NC_000962.3 Mycobrowser_v5 CDS 1073545 1073928 . + 0 Rv0960 vapC9 Unknown Possible toxin VapC9 Rv0960, (MTCY10D7.14c), len: 127 aa. Possible vapC9, toxin, part of toxin-antitoxin (TA) operon with Rv0959A, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. Rv0065|MTV030.08 (133 aa), FASTA scores: E(): 1.5e-14, (38.3% identity in 128 aa overlap), Rv1720c (129 aa), and Rv0549c (137 aa). P9WFA9 No virulence, detoxification, adaptation P64773 P9WFA9 P9WFA9 NC_000962.3 Mycobrowser_v5 CDS 801462 802133 . + 0 Rv0702 rplD This protein binds directly and specifically to 23S rRNA. 50S ribosomal protein L4 RplD Rv0702, (MTCY210.21), len: 223 aa. rplD, 50S ribosomal protein L4, equivalent to O06045|RL4_MYCBO 50S ribosomal protein L4 from Mycobacterium bovis BCG (223 aa); O06114|RL4_MYCSM 50S ribosomal protein L4 from Mycobacterium smegmatis (215 aa); and MLCB2492_3 50S ribosomal protein L4 from Mycobacterium leprae (230 aa). Also highly similar to others e.g. CAB82071.1|AL161803 50S ribosomal protein L4 from Streptomyces coelicolor (219 aa); P28601|RL4_BACST 50s ribosomal protein L4 from Bacillus stearothermophilus (207 aa), FASTA scores: opt: 522, E(): 3.5e-26, (42.4% identity in 198 aa overlap); etc. Belongs to the L4P family of ribosomal proteins. P9WH85 No information pathways P60729 P9WH85 GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WH85 NC_000962.3 Mycobrowser_v5 CDS 802133 802435 . + 0 Rv0703 rplW Binds to a specific region on the 23S rRNA. 50S ribosomal protein L23 RplW Rv0703, (MTCY21.22), len: 100 aa. rplW, 50S ribosomal protein L23, equivalent to O06046|RL23_MYCBO 50S ribosomal protein L23 from Mycobacterium bovis BCG (100 aa); and MLCB2492_4 50S ribosomal protein L23 from Mycobacterium leprae (100 aa). Also highly similar to others e.g. CAB82072.1|AL161803 50S ribosomal protein L23 from Streptomyces coelicolor (139 aa) (N-terminus longer); P04454|RL23_BACST 50s ribosomal protein L23 from Bacillus stearothermophilus (95 aa), FASTA scores: opt: 275, E(): 1.4e-13, (50.5% identity in 95 aa overlap); etc. Contains PS00050 Ribosomal protein L23 signature. Belongs to the L23P family of ribosomal proteins. P9WHB9 No information pathways P95051 P9WHB9 GO:0019843,GO:0005840,GO:0003735,GO:0006412,GO:0000166 P9WHB9 NC_000962.3 Mycobrowser_v5 CDS 802528 803370 . + 0 Rv0704 rplB This protein is a primary 23S rRNA-binding protein. It has peptidyltransferase activity. 50S ribosomal protein L2 RplB Rv0704, (MTCY210.23), len: 280 aa. rplB, 50S ribosomal protein L2, equivalent to O06047|RL2_MYCBO 50S ribosomal protein L2 from Mycobacterium bovis BCG (280 aa); and MLCB2492_5M 50S ribosomal protein L2 from Mycobacterium leprae (280 aa). Also highly similar to others e.g. CAB82073.1|AL161803 50S ribosomal protein L2 from Streptomyces coelicolor (278 aa); P42919|RL2_BACSU 50s ribosomal protein l2 (bl2) from Bacillus subtilis (276 aa), FASTA scores: opt: 1179, E(): 0, (61.1% identity in 275 aa overlap); etc. Contains PS00467 Ribosomal protein L2 signature. Belongs to the L2P family of ribosomal proteins. P9WHA5 No information pathways P95052 P9WHA5 GO:0019843,GO:0003735,GO:0016740,GO:0006412,GO:0015934 P9WHA5 NC_000962.3 Mycobrowser_v5 CDS 808746 809645 . + 0 Rv0712 Rv0712 Function unknown Conserved protein Rv0712, (MTCY210.31), len: 299 aa. Conserved protein, similar to others e.g. NP_106128.1|NC_002678 hypothetical protein from Mesorhizobium loti (372 aa); D90901_33|P72841 hypothetical 48.1 kDa protein from Synechocystis sp (410 aa), FASTA scores: E(): 1.1e-07, (28.8% identity in 299 aa overlap); etc. Slight similarity to carboxykinases. Similar to C-terminal part of Rv3703c conserved hypothetical protein from Mycobacterium tuberculosis (425 aa). I6Y8I5 No conserved hypotheticals P95060 I6Y8I5 I6Y8I5 NC_000962.3 Mycobrowser_v5 CDS 804282 805106 . + 0 Rv0707 rpsC This protein is involved in the binding of initiator met-tRNA. 30S ribosomal protein S3 RpsC Rv0707, (MTCY210.26), len: 274 aa. rpsC, 30S ribosomal protein S3, equivalent to O06048|RS3_MYCBO|MBS10OPER_8 30S ribosomal protein S3 from Mycobacterium bovis BCG (274 aa); and MLCB2492_8 30S ribosomal protein S3 from Mycobacterium leprae (281 aa). Also highly similar to others e.g. CAB82076.1|AL161803 30S ribosomal protein S3 from Streptomyces coelicolor (277 aa); P21465|RS3_BACSU 30s ribosomal protein s3 (bs3) (bs2) from Bacillus subtilis (217 aa), FASTA scores: opt: 794, E(): 0, (52.8% identity in 212 aa overlap); etc. Belongs to the S3P family of ribosomal proteins. P9WH37 No information pathways P0A5X6 P9WH37 GO:0015935,GO:0003735,GO:0006412,GO:0019843 P9WH37 NC_000962.3 Mycobrowser_v5 CDS 805110 805526 . + 0 Rv0708 rplP This protein binds directly to 23S ribosomal RNA and is located at the a site of the peptidyltransferase center. 50S ribosomal protein L16 RplP Rv0708, (MTCY210.27), len: 138 aa. rplP, 50S ribosomal protein L16, equivalent to O06049|RL16_MYCBO|MBS10OPER_9 50S ribosomal protein L16 from Mycobacterium bovis BCG (138 aa); and MLCB2492_9 50S ribosomal protein L16 from Mycobacterium leprae (138 aa). Also highly similar to others e.g. CAB82077.1|AL161803 50S ribosomal protein L16 from Streptomyces coelicolor (139 aa); P14577|RL16_BACSU 50s ribosomal protein l16 from Bacillus subtilis (144 aa), FASTA scores: opt: 600, E(): 0, (63.2% identity in 136 aa overlap); etc. Contains PS00701 Ribosomal protein L16 signature 2. Belongs to the L16P family of ribosomal proteins. P9WHD5 No information pathways P95056 P9WHD5 GO:0005840,GO:0003735,GO:0000049,GO:0006412,GO:0019843 P9WHD5 NC_000962.3 Mycobrowser_v5 CDS 805526 805759 . + 0 Rv0709 rpmC Involved in translation mechanisms. 50S ribosomal protein L29 RpmC Rv0709, (MTCY210.28), len: 77 aa. rpmC, 50S ribosomal protein L29, equivalent to O06050|RL29_MYCBO|MBS10OPER_10 50S ribosomal protein L29 from Mycobacterium bovis BCG (75 aa); and O32989|RL29_MYCLE|MLCB2492_10 50S ribosomal protein L29 from Mycobacterium leprae (80 aa). Also highly similar to others e.g. Q9L0D2|RL29_STRCO 50S ribosomal protein L29 from Streptomyces coelicolor (74 aa); P12873|RL29_BACSU 50s ribosomal protein l29 from Bacillus subtilis (66 aa), FASTA scores: opt: 225, E(): 8.3e-11, (58.6% identity in 58 aa overlap); etc. Belongs to the L29P family of ribosomal proteins. P9WHA7 No information pathways P95057 P9WHA7 GO:0003735,GO:0006412,GO:0005840 P9WHA7 NC_000962.3 Mycobrowser_v5 CDS 823683 824639 . - 0 Rv0731c Rv0731c Possible methyltransferase Possible S-adenosylmethionine-dependent methyltransferase Rv0731c, (MTV041.05c), len: 318 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), highly similar to other proteins from Mycobacterium tuberculosis e.g. Rv0726c|MTCY210.45c (367 aa), FASTA score: (60.9% identity in 317 aa overlap); Rv3399, Rv1729c, etc. P9WFI5 No lipid metabolism Q7D9D6 P9WFI5 GO:0008168 2.1.1.- P9WFI5 NC_000962.3 Mycobrowser_v5 CDS 805756 806166 . + 0 Rv0710 rpsQ Protein S17 binds specifically to the 5' end of 16S ribosomal RNA. 30S ribosomal protein S17 RpsQ Rv0710, (MTCY210.29), len: 136 aa. rpsQ, 30S ribosomal protein S17, equivalent to O06051|RS17_MYCBO 30S|MBS10OPER_11 30S ribosomal protein S17 from Mycobacterium bovis BCG (136 aa); and MLCB2492_11 30S ribosomal protein S17 from Mycobacterium leprae (126 aa). Also highly similar to others e.g. CAB82079.1|AL161803 30S ribosomal protein S17 from Streptomyces coelicolor (95 aa); P12874|RS17_BACSU 30s ribosomal protein s17 (bs 16) from Bacillus subtilis (86 aa), FASTA scores: opt: 305, E(): 1.6e-11, (60.5% identity in 81 aa overlap); etc. Contains PS00056 Ribosomal protein S17 signature. P9WH51 No information pathways P95058 P9WH51 GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WH51 NC_000962.3 Mycobrowser_v5 CDS 850741 851466 . - 0 Rv0756c Rv0756c Unknown Unknown protein Rv0756c, (MTCY369.01c), len: 241 aa. Unknown protein. P71813 No conserved hypotheticals P71813 NC_000962.3 Mycobrowser_v5 CDS 1052360 1052638 . + 0 Rv0942 Rv0942 Unknown Hypothetical protein Rv0942, (MTCY10D7.32c), len: 92 aa. Hypothetical unknown protein. P9WKN9 No conserved hypotheticals P9WKN9 P9WKN9 NC_000962.3 Mycobrowser_v5 CDS 2249478 2250974 . - 0 Rv2004c Rv2004c Unknown Conserved protein Rv2004c, (MTCY39.13), len: 498 aa. Conserved protein. Contains PS00017 ATP/GTP-binding site motif A. P9WLN3 No conserved hypotheticals P0A5F9 P9WLN3 GO:0005618,GO:0001906,GO:0005576,GO:0005524 NC_000962.3 Mycobrowser_v5 CDS 812627 812812 . + 0 Rv0717 rpsN1 Known to be required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the a site. 30S ribosomal protein S14 RpsN1 Rv0717, (MTCY210.36), len: 61 aa. rpsN1, 30S ribosomal protein S14, equivalent to MLCB2492_17|O32996 ribosomal protein S14 from Mycobacterium leprae (61 aa). Also highly similar to others e.g. CAB82083.1|AL161803 30S ribosomal protein S14 from Streptomyces coelicolor (61 aa); P24320|RS14_THETH 30s ribosomal protein S14 from Thermus aquaticus (subsp. thermophilus) (60 aa), FASTA scores: opt: 316, E(): 2e-19,(70.0% identity in 60 aa overlap); etc. Contains PS00527 Ribosomal protein S14 signature. Belongs to the S14P family of ribosomal proteins. Note that previously known as rpsN. P9WH57 No information pathways P0A5X2 P9WH57 GO:0019843,GO:0005840,GO:0003735,GO:0006412,GO:0046872 P9WH57 NC_000962.3 Mycobrowser_v5 CDS 811742 812059 . + 0 Rv0715 rplX This protein is found in the ribonucleoprotein core and is involved in the early assembly of the 50S subunit. It is not involved in the functions of the mature 50S subunit. 50S ribosomal protein L24 RplX Rv0715, (MTCY210.34), len: 105 aa. rplX, 50S ribosomal protein L24, equivalent to O32994|MLCB2492_15 50S ribosomal protein L24 from Mycobacterium leprae (105 aa). Also highly similar to others e.g. CAB82081.1|AL161803 50S ribosomal protein L24 from Streptomyces coelicolor (107 aa); P12876|RL24_BACSU 50s ribosomal protein L24 (bl23) from Bacillus subtilis (103 aa), FASTA scores: opt: 363, E(): 1.8e-18, (56.7% identity in 104 aa overlap); etc. Contains PS01108 Ribosomal protein L24 signature. Belongs to the L24P family of ribosomal proteins. P9WHB7 No information pathways P60627 P9WHB7 GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WHB7 NC_000962.3 Mycobrowser_v5 CDS 812059 812622 . + 0 Rv0716 rplE This is one of 3 proteins that mediate the attachment of the 5S RNA into the large ribosomal subunit. 50S ribosomal protein L5 RplE Rv0716, (MTCY210.35), len: 187 aa. rplE, 50S ribosomal protein L5, equivalent to MLCB2492_16 50S ribosomal protein L5 from Mycobacterium leprae (187 aa). Also highly similar to others e.g. CAB82082.1|AL161803 50S ribosomal protein L5 from Streptomyces coelicolor (185 aa); P33098|RL5_MICLU 50S ribosomal protein L5 from Micrococcus luteus (191 aa), FASTA scores: opt: 930, E(): 0, (73.8% identity in 183 aa overlap); etc. Belongs to the L5P family of ribosomal proteins. P9WH83 No information pathways P62403 P9WH83 GO:0005840,GO:0003735,GO:0000049,GO:0006412,GO:0019843 P9WH83 NC_000962.3 Mycobrowser_v5 CDS 813398 813937 . + 0 Rv0719 rplF This protein binds directly to 23S ribosomal RNA and is located at the aminoacyl-tRNA binding site of the peptidyltransferase center. 50S ribosomal protein L6 RplF Rv0719, (MTCY210.38), len: 179 aa. rplF, 50S ribosomal protein L6, equivalent to O32998|MLCB2492_19 50S ribosomal protein L6 from Mycobacterium leprae (179 aa). Also highly similar to others e.g. P46786|RL6_STRCO|CAB82085.1|AL161803|SCD31.42 50S ribosomal protein L6 from Streptomyces coelicolor (179 aa), FASTA scores: opt: 872, E(): 0, (70.4% identity in 179 aa overlap); etc. Contains PS00525 Ribosomal protein L6 signature 1. Belongs to the L6P family of ribosomal proteins. P9WH81 No information pathways P66311 P9WH81 GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WH81 NC_000962.3 Mycobrowser_v5 CDS 813940 814308 . + 0 Rv0720 rplR This is one of 3 proteins that mediate the attachment of the 5S RNA into the large ribosomal subunit. 50S ribosomal protein L18 RplR Rv0720, (MTCY210.39), len: 122 aa. rplR, 50S ribosomal protein L18, equivalent to O32999|MLCB2492_20|RL18_MYCLE 50S ribosomal protein L18 from Mycobacterium leprae (122 aa). Also highly similar to others e.g. CAB82086.1|AL161803 50S ribosomal protein L18 from Streptomyces coelicolor (127 aa); P33102|RL18_MICLU 50s ribosomal protein L18 from Micrococcus luteus (119 aa), FASTA scores: opt: 447, E(): 8.7e-24, (60.4% identity in 111 aa overlap); etc. Belongs to the L18P family of ribosomal proteins. P9WHD1 No information pathways P66076 P9WHD1 GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WHD1 NC_000962.3 Mycobrowser_v5 CDS 2250996 2251883 . - 0 Rv2005c Rv2005c Unknown Universal stress protein family protein Rv2005c, (MTCY39.12), len: 295 aa. Universal stress protein family protein. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WLN1 No virulence, detoxification, adaptation P64921 P9WLN1 GO:0006950 P9WLN1 NC_000962.3 Mycobrowser_v5 CDS 815190 815630 . + 0 Rv0723 rplO This protein binds directly to 23S ribosomal RNA. 50S ribosomal protein L15 RplO Rv0723, (MTCY210.42), len: 146 aa. rplO, 50S ribosomal protein L15, equivalent to MLCB2492_23|O33002 50S ribosomal protein L15 from Mycobacterium leprae (146 aa). Also highly similar to others e.g. P46787|RL15_STRCO|SCD31.46 50S ribosomal protein L15 from Streptomyces coelicolor (151 aa); P19946|RL15_BACSU 50s ribosomal protein L15 from Bacillus subtilis (146 aa), FASTA scores: opt: 419, E(): 6.5e-20, (51.0% identity in 145 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00475 Ribosomal protein L15 signature. Belongs to the L15P family of ribosomal proteins. P9WHD7 No information pathways P95071 P9WHD7 GO:0019843,GO:0003735,GO:0006412,GO:0015934 P9WHD7 NC_000962.3 Mycobrowser_v5 CDS 814993 815190 . + 0 Rv0722 rpmD Involved ion translation mechanisms. 50S ribosomal protein L30 RpmD Rv0722, (MTCY210.41), len: 65 aa. rpmD, 50S ribosomal protein L30, equivalent to O33001 ribosomal protein L30 from Mycobacterium leprae (71 aa). Also highly similar to others e.g. P46789|RL30_STRCO 50S ribosomal protein L30 from Streptomyces coelicolor (60 aa); P02430|RL30_ECOLI 50S ribosomal protein L30 from Escherichia coli (58 aa), FASTA scores: opt: 168, E(): 1.5e-13, (53.7% identity in 54 aa overlap); etc. Belongs to the L30P family of ribosomal proteins. P9WHA3 No information pathways P66181 P9WHA3 GO:0003735,GO:0006412,GO:0015934 P9WHA3 NC_000962.3 Mycobrowser_v5 CDS 830062 830610 . + 0 Rv0738 Rv0738 Function unknown Conserved protein Rv0738, (MTV041.12), len: 182 aa. Conserved protein, showing weak similarity with hypothetical proteins from Mycobacterium tuberculosis: Rv1727|MTCY04C12.12 (189 aa); MTY13D12_7|Z80343 hypothetical protein from Mycobacterium tuberculosis (194 aa), FASTA scores: opt: 172, E(): 0.0004, (24.2% identity in 178 aa overlap); and C-terminus of Rv0576. P9WKS3 No conserved hypotheticals P9WKS3 P9WKS3 NC_000962.3 Mycobrowser_v5 CDS 817539 818444 . - 0 Rv0725c Rv0725c Function unknown Conserved hypothetical protein Rv0725c, (MTCY210.44c), len: 301 aa. Conserved hypothetical protein, similar to hypothetical proteins from Mycobacterium tuberculosis e.g. Rv0726c, Rv0731c, Rv3399, etc, e.g. Y893_MYCTU|Q10552|Rv0893C hypothetical 36.1 kDa protein cy31.21c (325 aa), FASTA scores: opt: 600, E(): 3.9e-32, (43.8% identity in 219 aa overlap). P95073 No conserved hypotheticals P95073 P95073 GO:0008168 P95073 NC_000962.3 Mycobrowser_v5 CDS 818537 819640 . - 0 Rv0726c Rv0726c Possible methyltransferase Possible S-adenosylmethionine-dependent methyltransferase Rv0726c, (MTCY210.45c), len: 367 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), highly similar to other proteins from Mycobacterium tuberculosis e.g. Q10552|Y893_MYCTU|Rv0893c|MT0917|MTCY31.21c (325 aa), FASTA scores: opt: 646, E(): 0, (38.3% identity in 329 aa overlap); Rv0731c|MTV041.05c (318 aa), Rv3399, etc. Also similar to proteins from Mycobacterium leprae and other organisms e.g. T35930 hypothetical protein SC9B5.10 from Streptomyces coelicolor (303 aa). P9WFI7 No lipid metabolism P95074 P9WFI7 GO:0008168 2.1.1.- P9WFI7 NC_000962.3 Mycobrowser_v5 CDS 881459 882418 . + 0 Rv0787 Rv0787 Unknown Unknown protein Rv0787, (MTCY369.31), len: 319 aa. Unknown protein, equivalent to AAK45053.1 from Mycobacterium tuberculosis strain CDC1551 (242 aa) but longer 77 aa. P71840 No conserved hypotheticals P71840 NC_000962.3 Mycobrowser_v5 CDS 819843 820499 . - 0 Rv0727c fucA Involved in fucose metabolism (at the third step) [catalytic activity: L-fuculose 1-phosphate = glycerone phosphate + (S)-lactaldehyde]. Possible L-fuculose phosphate aldolase FucA (L-fuculose-1-phosphate aldolase) Rv0727c, (MTV41.01c, MTCY210.46c), len: 218 aa. Possible fucA, L-fuculose-1-phosphate aldolase, similar to many e.g. NP_386339.1|NC_003047 putative L-fuculose phosphate aldolase protein from Sinorhizobium meliloti (222 aa); P11550|FUCA_ECOLI L-fuculose phosphate aldolase from Escherichia strain K12 (215 aa), FASTA scores: opt: 372, E(): 4.1e-19, (34.6% identity in 185 aa overlap); etc. Belongs to the aldolase class II family, ARAD/FUCA subfamily. Cofactor: binds one zinc ion per molecule. P95075 No intermediary metabolism and respiration P95075 P95075 GO:0046872,GO:0008738 4.1.2.17 P95075 NC_000962.3 Mycobrowser_v5 CDS 820496 821476 . - 0 Rv0728c serA2 Involved at the first committed step in the 'phosphorylated' pathway of L-serine biosynthesis. Catalyzes the oxidation of D-3-phosphoglycerate to 3-phosphohydroxypyruvate [catalytic activity: 3-phosphoglycerate + NAD(+) = 3-phosphohydroxypyruvate + NADH]. Possible D-3-phosphoglycerate dehydrogenase SerA2 (phosphoglycerate dehydrogenase) (PGDH) Rv0728c, (MTV041.02c), len: 326 aa. Possible serA2, D-3-phosphoglycerate dehydrogenase, similar to others e.g. AF0278|AF027868_5|YoaD D-3-phosphoglycerate dehydrogenase from Bacillus subtilis (344 aa), FASTA scores: opt: 594, E(): 3.1e-31, (35.9% identity in 309 aa overlap); etc. Also similar to Rv2996c|MTV012.10|SERA1 D-3-phosphoglycerate dehydrogenase from Mycobacterium tuberculosis (528 aa). I6WZ71 No intermediary metabolism and respiration O86322 I6WZ71 GO:0055114,GO:0004617,GO:0051287 1.1.1.95 I6WZ71 NC_000962.3 Mycobrowser_v5 CDS 1616961 1617386 . - 0 Rv1439c Rv1439c Unknown Unknown protein Rv1439c, (MTCY493.15), len: 141 aa. Unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06820 No conserved hypotheticals O06820 O06820 O06820 NC_000962.3 Mycobrowser_v5 CDS 827543 828076 . + 0 Rv0735 sigL The sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released. Probable alternative RNA polymerase sigma factor SigL Rv0735, (MTV041.09), len: 177 aa. Probable sigL, alternative RNA polymerase sigma factor (rpoE) (see citations below), highly similar to many proteins of the extracytoplasmatic function (ECF) subfamily e.g. CAB72200.1|AL138851 putative RNA polymerase sigma factor from Streptomyces coelicolor (194 aa); Q06909|CARQ_MYXXA RNA polymerase sigma factor CARQ from Myxococcus xanthus (174 aa), FASTA scores: opt: 251, E(): 9.6e-11, (32.9% identity in 161 aa overlap); etc. Also similar to MTCI61_4, MTU87242_1, and MLU15180_30 from Mycobacterium tuberculosis. Contains PS01063 Sigma-70 factors ECF subfamily signature and probable helix-turn helix motif from aa 139-160 (Score 1134, +3.05 SD). Belongs to the sigma-70 factor family, ECF subfamily. P9WGH5 No information pathways 3HUG Q7D9D4 P9WGH5 GO:0016987,GO:0003700,GO:0006352,GO:0006355 P9WGH5 NC_000962.3 Mycobrowser_v5 CDS 2314825 2315061 . - 0 Rv2058c rpmB2 Involved in ribosome activity 50S ribosomal protein L28 RpmB2 Rv2058c, (MTCY63A.02), len: 78 aa. rpmB2, 50S ribosomal protein L28, very similar to rL28 of M. tuberculosis. Also similar to rpmB (Rv0105c) of Mycobacterium tuberculosis. P9WHA9 No information pathways P66148 P9WHA9 GO:0003735,GO:0006412,GO:0005840 P9WHA9 NC_000962.3 Mycobrowser_v5 CDS 826122 826667 . + 0 Rv0733 adk This small ubiquitous enzyme is essential in intracellular nucleotide metabolism, in addition it has been found to act as both a nucleoside mono- and DI-phosphate kinase suggesting it may have a role in RNA and DNA biosynthesis [catalytic activity: ATP + AMP = ADP + ADP]. Adenylate kinase Adk (ATP-AMP transphosphorylase) Rv0733, (MTV041.07), len: 181 aa. adk, adenylate kinase (ATP-AMP transphosphorylase), equivalent to Z98756|MLCB24 92_28 probable adenylate kinase from Mycobacterium leprae (181 aa), FASTA scores: opt: 978, E(): 0, (83.6% identity in 177 aa overlap); and AAF86323.1|AF271342 putative adenylate kinase from Mycobacterium marinum (124 aa) (N-terminus shorter). Also highly similar to others e.g. P43414|KAD_STRCO adenylate kinase from Streptomyces coelicolor (217 aa), FASTA score: (43.0% identity in 186 aa overlap); etc. Contains PS00113 Adenylate kinase signature. Belongs to the adenylate kinase family. P9WKF5 No intermediary metabolism and respiration 1P4S,2CDN P69440 P9WKF5 GO:0004017,GO:0005737,GO:0009165,GO:0005524 2.7.4.3 P9WKF5 NC_000962.3 Mycobrowser_v5 CDS 826670 827470 . + 0 Rv0734 mapA Removes the amino-terminal methionine from nascent proteins [catalytic activity: L-methionylpeptide + H2O = L-methionine + peptide]. Methionine aminopeptidase MapA (map) (peptidase M) (MetAP) Rv0734, (MTV041.08), len: 266 aa. mapA, methionine aminopeptidase (map), equivalent to Z98756|MLCB2492_29 probable methionine aminopeptidase from Mycobacterium leprae (266 aa), FASTA scores: opt: 1717, E(): 0, (83.4% identity in 265 aa overlap). Also highly similar to many e.g. T35553 methionine aminopeptidase from Streptomyces coelicolor (278 aa); etc. Also similar to Rv2861c|MAPB probable methionine aminopeptidase from Mycobacterium tuberculosis (285 aa). Belongs to peptidase family M24A; also known as the map family 1. Cofactor: cobalt; binds 2 ions per subunit. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P9WK21 No intermediary metabolism and respiration Q7D9D5 P9WK21 GO:0009987,GO:0046872,GO:0008235,GO:0006508,GO:0004177 3.4.11.18 P9WK21 NC_000962.3 Mycobrowser_v5 CDS 829207 829704 . + 0 Rv0737 Rv0737 Involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0737, (MTV041.11), len: 165 aa. Possible transcriptional regulator, similar to others e.g. BAB69161.1|AB070937 regulator protein from Streptomyces avermitilis (169 aa); NP_419731.1|NC_002696 transcriptional regulator MarR family from Caulobacter crescentus (148 aa) (homology only at C-terminus); etc. Also shows weak similarity to AB0014|AB001488_14 hypothetical protein from Bacillus subtilis (164 aa), FASTA scores: opt: 163, E(): 9.3e-05, (32.8% identity in 116 aa overlap), which is similar to slyY gene of S. typhimurium required for survival in macrophage. Contains possible helix-turn helix motif from aa 73-94 (Score 1138, +3.06 SD). I6Y8K3 No regulatory proteins Q7D9D2 I6Y8K3 GO:0006355,GO:0006350,GO:0003700,GO:0005622 I6Y8K3 NC_000962.3 Mycobrowser_v5 CDS 830855 831661 . + 0 Rv0739 Rv0739 Function unknown Conserved hypothetical protein Rv0739, (MTV041.13), len: 268 aa. Conserved hypothetical protein, showing some similarity to Mycobacterium tuberculosis proteins Rv0026 (448 aa), FASTA score: (37.6% identity in 101 aa overlap)and Rv0025 (120 aa), FASTA score: (32.4% identity in 142 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WKS1 No conserved hypotheticals O53802 P9WKS1 P9WKS1 NC_000962.3 Mycobrowser_v5 CDS 1595979 1596884 . + 0 Rv1421 Rv1421 Function unknown Conserved protein Rv1421, (MTCY21B4.39), len: 301 aa. Conserved protein, similar to many hypothetical proteins e.g. YHBJ_ECOLI|P33995 hypothetical 32.5 kd protein from Escherichia coli (284 aa), FASTA scores: opt: 648, E(): 6.3e-36, (38.7% identity in 282aa overlap). P9WFQ3 No conserved hypotheticals P67106 P9WFQ3 GO:0005525,GO:0005524 P9WFQ3 NC_000962.3 Mycobrowser_v5 CDS 2291269 2291925 . + 0 Rv2046 lppI Unknown Probable lipoprotein LppI Rv2046, (MTV018.33), len: 218 aa. Probable lppI, lipoprotein contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O53489 No cell wall and cell processes O53489 NC_000962.3 Mycobrowser_v5 CDS 2546102 2546431 . + 0 Rv2273 Rv2273 Unknown Probable conserved transmembrane protein Rv2273, (MTCY339.37c), len: 109 aa. Probable conserved transmembrane protein, similar to Rv2272 (MTCY339.38c), (35.0% identity in 100 aa overlap). P9WLF3 No cell wall and cell processes P64971 P9WLF3 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 832534 832848 . + 0 Rv0741 Rv0741 Required for the transposition of the insertion element IS1557. Probable transposase (fragment) Rv0741, (MTV041.15), len: 104 aa. Probable truncated transposase for IS1557, showing similarity to transposases and is elements e.g. U63997|EFU63997_1 insertion sequence from Enterococcus faecium (424 aa), FASTA score: (31.0% identity in 87 aa overlap). Very high similarity with the C-terminal part of Z73419|MTCY373_3 2 IS1557 from Mycobacterium tuberculosis (444 aa), FASTA score: (86.5% identity in 104 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6X9N4 No insertion seqs and phages I6X9N4 NC_000962.3 Mycobrowser_v5 CDS 832981 833508 . + 0 Rv0742 PE_PGRS8 Function unknown PE-PGRS family protein PE_PGRS8 Rv0742, (MTV041.16), len: 175 aa. PE_PGRS8, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), similar to many Mycobacterium tuberculosis PGRS-type proteins e.g. Z78020|MTCY1A11_25 (498 aa), FASTA scores: opt: 766, E(): 6.1e-25, (73.6% identity in 178 aa overlap). Similarity suggests ORF starts with ATA start codon. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6Y8K5 No PE/PPE Q79FW9 I6Y8K5 I6Y8K5 NC_000962.3 Mycobrowser_v5 CDS 833886 834443 . - 0 Rv0743c Rv0743c Unknown Hypothetical protein Rv0743c, (MTV041.17c), len: 185 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6WZ83 No conserved hypotheticals I6WZ83 I6WZ83 NC_000962.3 Mycobrowser_v5 CDS 835154 835681 . + 0 Rv0745 Rv0745 Function unknown Conserved hypothetical protein Rv0745, (MTV041.19), len: 175 aa. Conserved hypothetical protein; shows high similarity to a 50 aa region of Rv3649|Z95436|MTY15C10_3 conserved hypothetical protein, similar to ATP-dependent helicases, from Mycobacterium tuberculosis (771 aa), FASTA scores: opt: 225, E(): 7e-06, (70.0% identity in 50 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6X9N8 No conserved hypotheticals I6X9N8 NC_000962.3 Mycobrowser_v5 CDS 835701 838052 . + 0 Rv0746 PE_PGRS9 Function unknown PE-PGRS family protein PE_PGRS9 Rv0746, (MTV041.20), len: 783 aa. PE_PGRS9, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to part of MTCY28.25c|Rv1759c|Z95890 antigen wag22 from M. tuberculosis (914 aa), FASTA scores: opt: 2429, E(): 0, (56.9% identity in 873 aa overlap). Also similar to other PE-PGRS family proteins e.g. AL0212|MTV008_46 FASTA score: (48.8% identity in 887 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q79FW8 No PE/PPE Q79FW8 Q79FW8 Q79FW8 NC_000962.3 Mycobrowser_v5 CDS 1419014 1419748 . - 0 Rv1270c lprA Unknown Possible lipoprotein LprA Rv1270c, (MTCY50.12), len: 244 aa. Possible lprA, lipoprotein. Similar to O32852|AJ000500 lipoprotein from Mycobacterium bovis (236 aa), fasta scores: E(): 5.2e-23, (35.1% identity in 245 aa overlap). Similar to M. tuberculosis lipoproteins: Rv1368, Rv1411c, Rv2945c. Contains probable N-terminal signal sequence. P9WK55 No cell wall and cell processes Q11049 P9WK55 GO:0005886 P9WK55 NC_000962.3 Mycobrowser_v5 CDS 2314354 2314659 . - 0 Rv2056c rpsN2 Involved in translation 30S ribosomal protein S14 RpsN2 Rv2056c, (MTCY63A.04), len: 101 aa. rpsN2, 30S ribosomal protein S14, similar to many. Also similar to rpsN|Rv0717|MTCY210.36 from Mycobacterium tuberculosis, (50.0% identity in 62 aa overlap). P9WH59 No information pathways P66405 P9WH59 GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WH59 NC_000962.3 Mycobrowser_v5 CDS 840947 841204 . + 0 Rv0748 vapB31 Unknown Possible antitoxin VapB31 Rv0748, (MTV041.22), len: 85 aa. Possible vapB31, antitoxin, part of toxin-antitoxin (TA) operon with Rv0749, see Arcus et al. 2005. Also similar to others in Mycobacterium tuberculosis proteins e.g. Rv2871 (75 aa); Rv1241, Rv2132, Rv3321c, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53811 No virulence, detoxification, adaptation O53811 O53811 GO:0006355,GO:0003677 O53811 NC_000962.3 Mycobrowser_v5 CDS 841228 841656 . + 0 Rv0749 vapC31 Unknown Possible toxin VapC31. Contains PIN domain. Rv0749, (MTV041.23), len: 142 aa. Possible vapC31, toxin, part of toxin-antitoxin (TA) operon with Rv0748, contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. Rv0277c, Rv2530c, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WF75 No virulence, detoxification, adaptation O53812 P9WF75 P9WF75 NC_000962.3 Mycobrowser_v5 CDS 841737 841874 . - 0 Rv0749A Rv0749A Unknown Hypothetical protein (fragment) Rv0749A, len: 45 aa. Conserved hypothetical protein (probably gene fragment), similar to part (aa 250-292) of Rv2807|Z81331_12 from Mycobacterium tuberculosis (384 aa), FASTA scores: opt: 238, E(): 1.9e-13, (79.07% identity in 43 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6X9Q1 No conserved hypotheticals I6X9Q1 NC_000962.3 Mycobrowser_v5 CDS 1074074 1074421 . + 0 Rv0961 Rv0961 Unknown Probable integral membrane protein Rv0961, (MTCY10D7.13c), len: 115 aa. Probable integral membrane protein. P9WKM9 No cell wall and cell processes P9WKM9 GO:0005886,GO:0016021 P9WKM9 NC_000962.3 Mycobrowser_v5 CDS 843242 844414 . - 0 Rv0752c fadE9 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE9 Rv0752c, (MTV041.26c), len: 390 aa. Probable fadE9, acyl-CoA dehydrogenase, highly similar to many e.g. NP_437985.1|NC_003078 putative acyl-CoA dehydrogenase protein from Sinorhizobium meliloti (380 aa); Z99123|BSUB0020_14 from Bacillus subtilis (379 aa), FASTA scores: opt: 853, E(): 0, (39.8% identity in 384 aa overlap); etc. Contains PS00072 Acyl-CoA dehydrogenases signature 1, and PS00073 Acyl-Co Adehydrogenases signature 2. Belongs to the acyl-CoA dehydrogenases family. I6Y4R2 No lipid metabolism O53815 I6Y4R2 GO:0003995,GO:0055114,GO:0050660 1.3.99.- I6Y4R2 NC_000962.3 Mycobrowser_v5 CDS 863256 864143 . + 0 Rv0770 Rv0770 Function unknown; 3-hydroxyisobutyrate dehydrogenase family protein probably involved in cellular metabolism. Probable dehydrogenase/reductase Rv0770, (MTCY369.15), len: 295 aa. Probable dehydrogenase/reductase, 3-hydroxyisobutyrate dehydrogenase family, possibly 3-hydroxyisobutyrate dehydrogenase or 2-hydroxy-3-oxopropionate reductase, similar to others e.g. P23523|GARR_ECOLI 2-hydroxy-3-oxopropionate reductase (tartronate semialdehyde reductase) (TSAR) from Escherichia coli strain K12 (294 aa), FASTA scores: opt: 469, E(): 6.7e-22, (34.4% identity in 282 aa overlap); P28811|MMSB_PSEAE 3-hydroxyisobutyrate dehydrogenase (hibadh) from Pseudomonas aeruginosa (298 aa), FASTA scores: opt: 439, E(): 4.3e-20, (34.9% identity in 269 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. Rv1122 and Rv1844c. Seems to belong to the 3-hydroxyisobutyrate dehydrogenase family. P9WNY3 No intermediary metabolism and respiration P71825 P9WNY3 GO:0050662,GO:0055114,GO:0006098,GO:0004616,GO:0006573,GO:0008442 1.1.-.- P9WNY3 NC_000962.3 Mycobrowser_v5 CDS 2258854 2259285 . - 0 Rv2011c Rv2011c Unknown Conserved hypothetical protein, probable transcription repressor. Rv2011c, (MTCY39.06), len: 143 aa. Conserved hypothetical protein, probable transcription repressor. Contains IPR011991 Winged helix-turn-helix transcription repressor DNA-binding domain. P9WLM5 No regulatory proteins P9WLM5 P9WLM5 NC_000962.3 Mycobrowser_v5 CDS 846159 847913 . + 0 Rv0754 PE_PGRS11 Function unknown PE-PGRS family protein PE_PGRS11 Rv0754, (MTV041.28), len: 584 aa. PE_PGRS11, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), similar to others e.g. AL0212|MTV008_46 from Mycobacterium tuberculosis (1660 aa), FASTA score: (48.7% identity in 345 aa overlap); Z80225|MTCY441_4 from Mycobacterium tuberculosis (778 aa), FASTA score: (41.6% identity in 442 aa overlap); etc. Q79FW5 No PE/PPE Q79FW5 Q79FW5 Q79FW5 NC_000962.3 Mycobrowser_v5 CDS 848103 850040 . - 0 Rv0755c PPE12 Function unknown PPE family protein PPE12 Rv0755c, (MTV041.29), len: 645 aa. PPE12, Member of the Mycobacterium tuberculosis PPE family, highly similar to others e.g. Z82098|MTCY3C7_23 from Mycobacterium tuberculosis (582 aa), FASTA scores: (56.1% identity in 636 aa overlap); Z92774|MTCY6G11_5 from Mycobacterium tuberculosis (552 aa), FASTA scores: (55.8% identity in 590 aa overlap); etc. Predicted to be an outer membrane protein (See Song et al., 2008). P9WI37 No PE/PPE Q79FW4 P9WI37 P9WI37 NC_000962.3 Mycobrowser_v5 CDS 850342 850527 . - 0 Rv0755A Rv0755A Could be required for the transposition of an insertion element. Putative transposase (fragment) Rv0755A, len: 61 aa. Putative transposase (possibly gene fragment), similar to C-terminal part of Q9EZM2|ISMav2|AF286339_1 putative transposase from Mycobacterium paratuberculosis (395 aa), FASTA scores: opt: 284, E(): 5e-13, (83.02% identity in 53 aa overlap); and to SCJ11.25c|Q9RI80 possible noncomposite transposon transposase from Streptomyces coelicolor (283 aa). Q79FW3 No insertion seqs and phages Q79FW3 Q79FW3 GO:0004803,GO:0006313,GO:0003677 NC_000962.3 Mycobrowser_v5 CDS 868984 869763 . - 0 Rv0776c Rv0776c Function unknown Conserved hypothetical protein Rv0776c, (MTCY369.21a), len: 259 aa. Conserved hypothetical protein, similar (except first 50 aa) to P72737|D90900_57 hypothetical protein from Synechocystis sp. strain PCC 6803 (261 aa), FASTA scores: opt: 337, E(): 1.7e-15, (30.5% identity in 266 aa overlap). I6Y4S9 No conserved hypotheticals P71831 I6Y4S9 NC_000962.3 Mycobrowser_v5 CDS 878638 879324 . + 0 Rv0784 Rv0784 Function unknown Conserved hypothetical protein Rv0784, (MTC369.28), len: 228 aa. Conserved hypothetical protein, with some similarity to MLCB5_20|O05752 hypothetical protein from Mycobacterium leprae (193 aa), FASTA scores: opt: 141, E(): 0.0022, (36.0% identity in 114 aa overlap). Also similar to N-terminus of NP_253002.1|NC_002516 conserved hypothetical protein from Pseudomonas aeruginosa (253 aa). P71837 No conserved hypotheticals P71837 P71837 NC_000962.3 Mycobrowser_v5 CDS 853825 854157 . - 0 Rv0759c Rv0759c Function unknown Conserved hypothetical protein Rv0759c, (MTCY369.04c), len: 110 aa. Conserved hypothetical protein, highly similar (but shorter 45 aa in N-terminus) to P49774|YHIT_MYCLE|ML2237|MLCB5.04c|U296A hypothetical hit-like protein from Mycobacterium leprae (155 aa), FASTA scores: opt: 766, E(): 0, (78.7% identity in 150 aa overlap). Also highly similar (but N-terminus always shorter) to hit-like proteins and protein kinase inhibitors e.g. AAF72728.1|AF265258_1|AF265258 hit-like protein from Rhodococcus sp. (141 aa); NP_212513.1|NC_001318 protein kinase C1 inhibitor (pkcI) from Borrelia burgdorferi (149 aa) ; P94252|YHIT_BORBU|BB0379 hypothetical hit-like protein from Borrelia burgdorferi (139 aa); NP_110768.1|NC_002689 hit (histidine triad) family protein from Thermoplasma volcanium (158 aa); P16436|IPK1_BOVIN protein kinase C inhibitor 1 (pkci-1) from Bos taurus (Bovine) (125 aa), FASTA scores: opt: 195, E(): 5.2e-08, (33.3% identity in 111 aa overlap); etc. Also shows similarity with Rv2613c|MTCY01A10.20A conserved hypothetical protein from Mycobacterium tuberculosis (195 aa) and Rv1262c|MTCY50.20 hypothetical hit-like protein (144 aa). P9WML3 No conserved hypotheticals P0A5B5 P9WML3 GO:0003824 P9WML3 NC_000962.3 Mycobrowser_v5 CDS 854267 854686 . - 0 Rv0760c Rv0760c Function unknown Conserved protein Rv0760c, (MTCY369.05), len: 139 aa. Conserved protein, similar to N-terminal part of Rv2042c conserved hypothetical protein from Mycobacterium tuberculosis (265 aa), FASTA scores: opt: 150, E(): 4.1e-05, (28.7% identity in 136 aa overlap). Belongs to the NTF2-like (nuclear transport factor 2) protein superfamiily. I6WZD7 No conserved hypotheticals 2A15,2Z76,2Z77,2Z7A P71817 I6WZD7 GO:0016853,GO:0006810,GO:0005622 I6WZD7 NC_000962.3 Mycobrowser_v5 CDS 855925 856470 . - 0 Rv0762c Rv0762c Function unknown Conserved hypothetical protein Rv0762c, (MTCY369.07c), len: 181 aa. Conserved hypothetical protein, showing weak similarity to D90907_77|P73575 hypothetical 31.3KD protein from Synechocystis sp, FASTA scores: E(): 0.0012, (30.4% identity in 92 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). I6XW93 No conserved hypotheticals I6XW93 I6XW93 NC_000962.3 Mycobrowser_v5 CDS 867440 868351 . - 0 Rv0774c Rv0774c Unknown; could have possibly a lipolytic activity. Probable conserved exported protein Rv0774c, (MTCY369.19c), len: 303 aa. Possible conserved exported protein with hydrophobic region near N-terminus, highly similar, except in N-terminus, to Rv0519c|Z97831|MTY20G10.09c|O33364 hypothetical protein from Mycobacterium tuberculosis (300 aa), FASTA scores: opt: 1092, E(): 0, (57.9% identity in 299 aa overlap). Contains PS00061 Short-chain alcohol dehydrogenase family signature, and PS00120 Lipases, serine active site. So could be a lipase. Start changed since first submission (-9 aa). Predicted to be an outer membrane protein (See Song et al., 2008). I6Y8R4 No cell wall and cell processes P71829 I6Y8R4 I6Y8R4 NC_000962.3 Mycobrowser_v5 CDS 1243010 1243504 . - 0 Rv1120c Rv1120c Function unknown Conserved hypothetical protein Rv1120c, (MTCY22G8.09c), len: 164 aa. Conserved hypothetical protein, some similarity at C-terminus to Mycobacterium tuberculosis hypothetical proteins e.g. Rv1890c|MTCY180.28 (462 aa), FASTA scores: opt: 187, E(): 2.2e-05, (36.6% identity in 93 aa overlap) and Rv2488c|YZ19_MYCTU|Q10551 (285 aa), FASTA scores: opt: 156, E(): 0.00074, (32.7% identity in 107 aa overlap). O06572 No conserved hypotheticals O06572 O06572 GO:0016849,GO:0009190 O06572 NC_000962.3 Mycobrowser_v5 CDS 856473 856679 . - 0 Rv0763c Rv0763c Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Probably involved in electron transport for cytochrome P-450 system. Possible ferredoxin Rv0763c, (MTCY369.08c), len: 68 aa. Possible ferredoxin, similar to others and related proteins e.g. P18324|FER1_STRGO|SUAB ferredoxin 1 (fd-1) from Streptomyces griseolus (68 aa); AAK31349.1|AF350429_2|AF350429 putative ferredoxin from Nocardioides sp (63 aa); AAK16536.1|AF331043_16|AF331043 phthalate dioxygenase ferredoxin subunit from Arthrobacter keyseri (64 aa); etc. Probably involved in electron transport for cytochrome P-450 system e.g. downstream ORF Rv0764c|MTCY369.09c probable cytochrome P450 51 from Mycobacterium tuberculosis (451 aa), FASTA scores: opt: 137, E(): 0.00013, (36.4% identity in 66 aa overlap). Also similar to putative ferredoxins Rv3503c and Rv1786 from Mycobacterium tuberculosis. Could belong to the bacterial type ferredoxin family. P71820 No intermediary metabolism and respiration P71820 P71820 P71820 NC_000962.3 Mycobrowser_v5 CDS 858037 858864 . - 0 Rv0765c Rv0765c Function unknown; probably involved in cellular metabolism, possibly electron transfer. Probable oxidoreductase Rv0765c, (MTCY369.10c), len: 275 aa. Probable oxidoreductase, similar others e.g. P39071|DHBA_BACSU 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase from Bacillus subtilis (261 aa), FASTA scores: opt: 385, E(): 1.8e-17, (30.6% identity in 252 aa overlap); AAF81239.1|AF263012 putative beta-ketoacyl reductase from Streptomyces griseus (274 aa); NP_436514.1|NC_003037 putative oxidoreductase from Sinorhizobium meliloti (240 aa); etc. Also similar to several other oxidoreductases from Mycobacterium tuberculosis e.g. Rv1544|MTCY48.21, FASTA score: (32.6% identity in 267 aa overlap); etc. Contains PS00061 Short-chain alcohol dehydrogenase family signature. I6WZD9 No intermediary metabolism and respiration P71821 I6WZD9 GO:0055114,GO:0016491 1.-.-.- I6WZD9 NC_000962.3 Mycobrowser_v5 CDS 4400870 4401634 . + 0 Rv3912 Rv3912 Unknown Hypothetical alanine rich protein Rv3912, (MTV008.03), len: 254 aa. Hypothetical unknown ala-rich protein. Cleaved by Rip|Rv2869c, in M. tuberculosis Erdman (See Sklar et al., 2010). P9WJ65 No conserved hypotheticals P9WJ65 NC_000962.3 Mycobrowser_v5 tRNA 923803 923875 . - 0 MTB000010 lysT - tRNA-Lys (TTT) lysT, tRNA-Lys, anticodon ttt, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 923999 924072 . + 0 MTB000011 gluT - tRNA-Glu (TTC) gluT, tRNA-Glu, anticodon ttc, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 858864 860072 . - 0 Rv0766c cyp123 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Probable cytochrome P450 123 Cyp123 Rv0766c, (MT0790, MTCY369.11c), len: 402 aa. Probable cyp123, cytochrome P-450, similar to others e.g. P33271|CPXK_SACER cytochrome P-450 107B1 from Saccharopolyspora erythraea (405 aa), FASTA scores: opt: 770, E(): 0, (36.9% identity in 406 aa overlap); T36526 probable cytochrome P450 hydroxylase from Streptomyces coelicolor (411 aa); P27632|CPXM_BACSU cytochrome P450 109 from Bacillus subtilis (405 aa); etc. Also similar to several other cytochromes P-450 from Mycobacterium tuberculosis e.g. Rv1256c|MTCY50.26 (405 aa), FASTA score: (35.2% identity in 389 aa overlap); etc. Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. Belongs to the cytochrome P450 family. P9WPP5 No intermediary metabolism and respiration P63707 P9WPP5 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPP5 NC_000962.3 Mycobrowser_v5 CDS 860069 860710 . - 0 Rv0767c Rv0767c Function unknown Conserved hypothetical protein Rv0767c, (MTCY369.12c), len: 213 aa. Conserved hypothetical protein, showing weak similarity with AL133220|SCC75A_26 hypothetical protein from Streptomyces coelicolor (215 aa), FASTA scores: opt: 152, E(): 0.0048, (28.4% identity in 204 aa overlap). P9WMD7 No conserved hypotheticals P67432 P9WMD7 GO:0006350,GO:0003700,GO:0006355 P9WMD7 NC_000962.3 Mycobrowser_v5 CDS 864140 864574 . + 0 Rv0771 Rv0771 Involved in aromatic hydrocarbons catabolism. Thought to be involved in the catabolism of protocatechuate to succinate-and acetyl-CoA in the beta-ketoadipate pathway (at the third step) [catalytic activity: 2-carboxy-5-oxo-2,5-dihydrofuran-2-acetate = 5-oxo-4,5-dihydrofuran-2-acetate + CO(2)]. Possible 4-carboxymuconolactone decarboxylase (CMD) Rv0771, (MTCY369.16), len: 144 aa. Possible 4-carboxymuconolactone decarboxylase, showing similarity with other carboxymuconolactone decarboxylases e.g. AAD39557.1|AF031417 PcaC-like protein from Pseudomonas putida (130 aa); P20370|DC4C_ACICA 4-carboxymuconolactone decarboxylase (CMD) from Acinetobacter sp. ADP1 (134 aa), FASTA scores: opt: 174, E(): 0.00075, (31.4% identity in 121 aa overlap); C-terminus of NP_421214.1|NC_002696 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase from Caulobacter crescentus (393 aa); C-terminus of T47115 probable 4-carboxymuconolactone decarboxylase / 3-oxoadipate enol-lactone hydrolase from Streptomyces sp (373 aa); NP_407104.1|NC_003143 putative gamma carboxymuconolactone decarboxylase from Yersinia pestis (131 aa); etc. I6Y4S7 No intermediary metabolism and respiration P71826 I6Y4S7 GO:0047575 4.1.1.44 I6Y4S7 NC_000962.3 Mycobrowser_v5 CDS 865851 867389 . - 0 Rv0773c ggtA Besides the cephalosporin acylase I activity which converts GL-7ACA into 7-ACA; this enzyme displays some gamma glutamyltranspeptidase activity: GGT plays a key role in the gamma-glutamyl cycle, a pathway for the synthesis and degradation of glutathione. [catalytic activity 1: 7-beta-(4-carboxybutanamido)-cephalosporanic acid + H2O = 7-aminocephalosporanic acid + glutaric acid] [catalytic activity 2: (5-L-glutamyl)-peptide + an amino acid = peptide + 5-L-glutamyl-amino acid]. Probable bifunctional acylase GgtA: cephalosporin acylase (GL-7ACA acylase) + gamma-glutamyltranspeptidase (GGT) Rv0773c, (MTCY369.18), len: 512 aa. Probable ggtA, bifunctional acylase including cephalosporin acylase, and gamma-glutamyl transpeptidase; highly similar to others e.g. NP_295247.1|NC_001263 cephalosporin acylase from Deinococcus radiodurans (535 aa); NP_248854.1|NC_002516 probable gamma-glutamyltranspeptidase from Pseudomonas aeruginosa (538 aa); P15557|PAC1_PSES3 acylase ACY 1 [includes: cephalosporin acylase (GL-7ACA acylase); gamma-glutamyltranspeptidase (GGT)] from Pseudomonas sp. strain SE83 (558 aa), FASTA scores: opt: 784, E(): 0, (34.2% identity in 526 aa overlap); NP_391491.1|NC_000964|Z93767|BSZ93767_6|O0521 protein similar to gamma-glutamyltransferase from Bacillus subtilis (525 aa), FASTA scores: opt: 1169, E(): 0, (40.1% identity in 516 aa overlap); etc. Also similar to Rv2394|ggtB from Mycobacterium tuberculosis. Member of GL-7ACA acylases and to GGT group. I6X9S5 No intermediary metabolism and respiration P71828 I6X9S5 GO:0003840,GO:0016787,GO:0008415 3.5.1.-,2.3.2.2 I6X9S5 NC_000962.3 Mycobrowser_v5 CDS 868407 869030 . + 0 Rv0775 Rv0775 Function unknown Conserved hypothetical protein Rv0775, (MTCY369.20), len: 207 aa. Conserved hypothetical protein, showing some similarity to other proteins e.g. ECAE000186_11|MG1655 hypothetical protein from Escherichia coli strain K-12 (178 aa), FASTA scores: E(): 6.4e-05, (27.2% identity in 147 aa overlap); P41037|BIH_ECOLI hypothetical transcriptional regulator from Escherichia coli (103 aa), FASTA scores: opt: 138, E(): 0.003, (30.9% identity in 97 aa overlap); etc. P71830 No conserved hypotheticals P71830 P71830 GO:0006350,GO:0003700,GO:0006355 P71830 NC_000962.3 Mycobrowser_v5 CDS 2330214 2330963 . - 0 Rv2073c Rv2073c Unknown Probable shortchain dehydrogenase Rv2073c, (MTCY49.12c), len: 249 aa. Probable oxidoreductase, belonging to shortchain dehydrogenase reductase (SDR) family, similar to many. P9WGR3 No intermediary metabolism and respiration Q10681 P9WGR3 GO:0055114,GO:0016491 1.-.-.- P9WGR3 NC_000962.3 Mycobrowser_v5 CDS 894631 894978 . + 0 Rv0801 Rv0801 Function unknown Conserved protein Rv0801, (MTCY07H7A.08c), len: 115 aa. Conserved protein, similar to many hypothetical proteins from Streptomyces sp. e.g. SCD840A.20|AB81865.1|AL161691 hypothetical protein from Streptomyces coelicolor (145 aa); AF072709|AF072709_8 from Streptomyces lividans (131 aa), FASTA scores: opt: 120, E(): 0.2, (26.3% identity in 118 aa overlap); etc. O06633 No conserved hypotheticals O06633 O06633 NC_000962.3 Mycobrowser_v5 CDS 871431 872675 . + 0 Rv0778 cyp126 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Possible cytochrome P450 126 Cyp126 Rv0778, (MT0802, MTCY369.22), len: 414 aa. Possible cyp126, cytochrome P-450, similar to other cytochromes and related proteins e.g. AAG29781.1|AF235050_4|AF235050 cytochrome P-450 from Streptomyces rishiriensis (407 aa); Q59723|PSECYTOCHR_1 cytochrome p-450 linalool 8-monooxygenase (lin C) from Pseudomonas incognita (406 aa), FASTA scores: opt: 769, E(): 0, (37.0% identity in 411 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. Rv0766c, Rv2266, Rv3545c, etc. Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. P9WPN9 No intermediary metabolism and respiration P63711 P9WPN9 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPN9 NC_000962.3 Mycobrowser_v5 CDS 872672 873292 . - 0 Rv0779c Rv0779c Unknown Possible conserved transmembrane protein Rv0779c, (MTCY369.23c), len: 206 aa. Possible conserved transmembrane protein, equivalent to Z95151|MLCB5_14 O05747 conserved hypothetical protein from Mycobacterium leprae (206 aa), FASTA scores: opt: 902, E(): 0, (67.2% identity in 204 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P71833 No cell wall and cell processes P71833 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 889072 889398 . + 0 Rv0795 Rv0795 Required for the transposition of the insertion element IS6110. Putative transposase for insertion sequence element IS6110 (fragment) Rv0795, (MTV042.05), len: 108 aa. Putative transposase for IS6110 (fragment), identical to Q50686 insertion element IS6110 (108 aa), FASTA score: (100.0 % identity in 108 aa overlap). The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv0795 and Rv0796, the sequence UUUUAAAG (directly upstream of Rv0796) maybe responsible for such a frameshifting event (see McAdam et al., 1990). P9WKH5 No insertion seqs and phages P0C5G9 P9WKH5 GO:0004803,GO:0006313,GO:0003677 P9WKH5 NC_000962.3 Mycobrowser_v5 CDS 910972 911739 . + 0 Rv0818 Rv0818 Involved in transcriptional mechanism. Transcriptional regulatory protein Rv0818, (MTV043.10), len: 255 aa. Probable transcriptional regulatory protein, highly similar to Q05943|GLNR_STRCO|L03213|STMGLNR_1|SCD84.26c transcriptional regulatory protein from Streptomyces coelicolor (267 aa), FASTA scores: opt: 945, E(): 0, (61.5 identity in 239 aa overlap); and similar to others from other organisms. Also similar to Rv2884|MTCY274.15|Z74024 from Mycobacterium tuberculosis (252 aa), FASTA scores: opt: 662, E(): 0, (47.8% identity in 226 aa overlap). O53830 No regulatory proteins O53830 O53830 GO:0005622,GO:0006355,GO:0000156,GO:0000160,GO:0003677 O53830 NC_000962.3 Mycobrowser_v5 CDS 1029513 1030577 . - 0 Rv0923c Rv0923c Function unknown Conserved hypothetical protein Rv0923c, (MTCY21C12.17c), len: 354 aa. Conserved hypothetical protein, showing similarity with C-terminal part of AF034138|AF034138_7|yjoB hypothetical protein from Bacillus subtilis (200 aa), FASTA scores: opt: 193, E(): 4.2e-05, (32.3% identity in 167 aa overlap). I6XWK6 No conserved hypotheticals O05915 I6XWK6 I6XWK6 NC_000962.3 Mycobrowser_v5 CDS 874732 876390 . + 0 Rv0782 ptrBb Cleaves peptide bonds on the C-terminal side of lysyl and argininyl residues [catalytic activity: hydrolysis of ARG-|-XAA and LYS-|-XAA bonds in oligopeptides, even when P1' residue is proline]. Probable protease II PtrBb [second part] (oligopeptidase B) Rv0782, (MTCY369.26), len: 552 aa. Probable ptrBb, second part of protease II, equivalent to C-terminus of NP_302455.1|NC_002677 protease II from Mycobacterium leprae (724 aa). Also highly similar to N-termini of many proteases II e.g. P24555|PTRB_ECOLI|TLP|B1845 protease II from Escherichia coli strains K12 and HB101 (707 aa), FASTA scores: opt: 1251, E(): 0, (42.7% identity in 489 aa overlap); etc. ORFs Rv0782 and Rv0781 appear to be a frameshifted homologues of protease II, but we can find no error in the cosmid sequence to account for this. Belongs to peptidase family S9A; also known as the prolyl oligopeptidase family. Note that previously known as ptrBa. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P71834 No intermediary metabolism and respiration P71834 P71834 GO:0004252,GO:0006508 3.4.21.83 P71834 NC_000962.3 Mycobrowser_v5 CDS 882760 883434 . + 0 Rv0788 purQ Involved in de novo purine biosynthesis (at the fourth step) [catalytic activity: ATP + 5'-phosphoribosylformylglycinamide + L-glutamine + H2O = ADP + phosphate + 5'-phosphoribosylformylglycinamidine + L-glutamate]. Probable phosphoribosylformylglycinamidine synthase I PURG (FGAM synthase I) Rv0788, (MTCY369.32), len: 224 aa. Probable purQ, phosphoribosylformylglycinamidine synthase I, equivalent to MLCB5_24|Z95151|O05756|PURQ phosphoribosylformylglycinamidine synthase I from Mycobacterium leprae (224 aa), FASTA scores: opt: 1341, E(): 0, (88.7% identity in 222 aa overlap). Also highly similar to others e.g. P12041|PURQ_BACSU phosphoribosylformylglycinamidine synthase I from Bacillus subtilis (227 aa), FASTA scores: opt: 691, E(): 8.6e-39, (47.7% identity in 214 aa overlap); etc. Contains PS00442 Glutamine amidotransferases class-I active site. Belongs to type-1 glutamine amidotransferases. P9WHL5 No intermediary metabolism and respiration P65902 P9WHL5 GO:0005524,GO:0005737,GO:0006541,GO:0004642,GO:0006189 6.3.5.3 P9WHL5 NC_000962.3 Mycobrowser_v5 CDS 884797 885840 . - 0 Rv0791c Rv0791c Function unknown Conserved protein Rv0791c, (MTV042.01c, MTCY369.35c), len: 347 aa. Conserved protein, similar (except in N-terminus) to others e.g. CAC44585.1|AL596162 conserved hypothetical protein from Streptomyces coelicolor (307 aa); NP_252643.1|NC_002516 hypothetical protein from Pseudomonas aeruginosa (364 aa); etc. Also some similarity with oxidoreductases e.g. AAK38097.1|AF323606_3|AF323606 putative F420-dependent dehydrogenase from Rhodococcus erythropolis (295 aa); etc. And also similar in part to other proteins from Mycobacterium tuberculosis e.g. Rv1855c|MTCY359.18|Z83859 (307 aa), FASTA scores: opt: 366, E(): 4e-16, (35.0% identity in 226 aa overlap); Rv3079c|MTCY22D7.02|Z83866 conserved hypothetical protein (275 aa), FASTA scores: opt: 342, E(): 1.2e-14, (31.6% identity in 234 aa overlap); Rv0044c possible oxidoreductase (264 aa). I6X9T8 No conserved hypotheticals P71844 I6X9T8 GO:0016491,GO:0055114 I6X9T8 NC_000962.3 Mycobrowser_v5 CDS 885837 886646 . - 0 Rv0792c Rv0792c Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably GntR-family) Rv0792c, (MTV042.02c), len: 269 aa. Probable transcriptional regulator, GntR-family, similar to many others of GntR family e.g. BSUB0018_189|Z99121 from Bacillus subtilis (243 aa), FASTA scores: opt: 367, E(): 1.5e-17, (32.1% identity in 246 aa overlap); P31453|YIDP_ECOLI from Escherichia coli (238 aa), FASTA scores: opt: 236, E(): 8.8e-09, (26.4% identity in 235 aa overlap); etc. O86331 No regulatory proteins O86331 O86331 GO:0006355,GO:0006350,GO:0003700,GO:0005622 O86331 NC_000962.3 Mycobrowser_v5 CDS 886719 887024 . + 0 Rv0793 Rv0793 Function unknown Possible monooxygenase Rv0793, (MTV042.03), len: 101 aa. Possible monooxygenase (See Lemieux et al., 2005). Similar to e.g. NP_250888.1|NC_002516 hypothetical protein from Pseudomonas aeruginosa (114 aa); AE 001908|AE001908_7 hypothetical protein from Deinococcus radiodurans (101 aa), FASTA scores: opt: 215, E(): 3.1e-09, (40.4% identity in 99 aa overlap); NP_440966.1|NC_000911|D90908|PCC6803|D90908_2 unknown protein from Synechocystis sp. strain PCC 6803 (147 aa), FASTA scores: opt: 194, E(): 4.5e-08, (31.1% identity in 90 aa overlap); etc. Also similar to Rv2749|MTV002.14|AL0089|MTV002_15 conserved hypothetical protein from Mycobacterium tuberculosis (104 aa), FASTA scores: opt: 143, E(): 0.00026, (26.9% identity in 93 aa overlap). O86332 No intermediary metabolism and respiration 1Y0H O86332 O86332 GO:0005737,GO:0016491,GO:0017000 O86332 NC_000962.3 Mycobrowser_v5 CDS 922894 923709 . - 0 Rv0831c Rv0831c Function unknown Conserved protein Rv0831c, (MTV043.23c), len: 271 aa. Conserved protein, similar to Rv0347|MTY13E10_7|Z95324 conserved hypothetical protein from Mycobacterium tuberculosis (328 aa), FASTA scores: opt: 426, E(): 2.6e-21, (33.6% identity in 262 aa overlap). O53842 No conserved hypotheticals O53842 NC_000962.3 Mycobrowser_v5 CDS 4118530 4118691 . - 0 Rv3678A Rv3678A Function unknown Conserved hypothetical protein Rv3678A, len: 53 aa. Conserved hypothetical protein, similar to SCH17.10|AL079353_10 conserved hypothetical protein from Streptomyces coelicolor (53 aa), FASTA scores: opt: 259, E(): 1.5e-13, (78.0% identity in 50 aa overlap). I6X824 No conserved hypotheticals I6X824 I6X824 NC_000962.3 Mycobrowser_v5 CDS 890388 891482 . + 0 Rv0797 Rv0797 Required for the transposition of the insertion element IS1547. Putative transposase for insertion sequence element IS1547 Rv0797, (MTCI249B.03c, MTV042.07), len: 364 aa. Putative transposase for IS1547; almost identical to (but 20 aa shorter than) Y13470|MTY13470_2 from Mycobacterium tuberculosis (383 aa). Also similar to other transposases e.g. MAIS1110A _1|Q48909 transposase from Mycobacterium avium (464 aa), FASTA scores: opt: 226, E(): 2.4e-08, (30.7% identity in 199 aa overlap). Also slight similarity to Rv2014|MTCY39.03c from Mycobacterium tuberculosis (222 aa), FASTA score: (24.8% identity in 141 aa overlap). O07182 No insertion seqs and phages O07182 O07182 GO:0004803,GO:0006313,GO:0003677 O07182 NC_000962.3 Mycobrowser_v5 CDS 891472 892269 . - 0 Rv0798c cfp29 Unknown. Supposedly released from the envelope to the outside during growth. 29 KDa antigen CFP29 Rv0798c, (MTCI429B.02), len: 265 aa. Cfp29, 29 kDa antigen (see citations below). Highly similar to Q45296|BLLINM18P_1|CAA63787.1|X93588 linocin M18 from Brevibacterium linens (266 aa), FASTA scores: (58.5% identity in 265 aa overlap). Also shows similarity with NP_228594.1|NC_000853 bacteriocin from Thermotoga maritima (262 aa). I6WZG6 No virulence, detoxification, adaptation O07181 I6WZG6 GO:0008233,GO:0042742 I6WZG6 NC_000962.3 Mycobrowser_v5 CDS 893318 894619 . + 0 Rv0800 pepC Function unknown; possibly hydrolyzes peptides and/or proteins. Probable aminopeptidase PepC Rv0800, (MTCY07H7A.09c), len: 433 aa. Probable pepC, aminopeptidase I, highly similar (but shorter 17 aa) to Q50022|PEPX aminopeptidase from Mycobacterium leprae (443 aa), FASTA scores: opt: 2237, E(): 0, (78.3% identity in 433 aa overlap). Also highly similar to others from Eukaryotes and bacteria, e.g. T36482 probable aminopeptidase from Streptomyces coelicolor (432 aa), P14904|AMPL_YEAST vacuolar aminopeptidase I precursor from Saccharomyces cerevisiae (514 aa), FASTA scores: opt: 425, E(): 4.8e-21, (31.0% identity in 445 aa overlap); etc. Also similar to hypothetical proteins e.g. P38821|YHR3_YEAST hypothetical 54.2 kDa protein from Saccharomyces cerevisiae (490 aa), FASTA scores: opt: 429, E(): 2.5e-21, (34.8% identity in 443 aa overlap); etc. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P9WHT1 No intermediary metabolism and respiration O06634 P9WHT1 GO:0008237,GO:0006508,GO:0005773,GO:0008270,GO:0004177 3.4.11.- P9WHT1 NC_000962.3 Mycobrowser_v5 CDS 894972 895628 . - 0 Rv0802c Rv0802c Succinylation, substrate unknown Possible succinyltransferase in the GCN5-related N-acetyltransferase family Rv0802c, (MTCY07H7A.07c), len: 218 aa. Possible succinyltransferase in the GNAT (Gcn5-related N-acetyltransferase) family (See Vetting et al., 2008). Shows partial similarity with many acetyltransferases and hypothetical proteins e.g. P96579|BSUB0003_68 probable acetyltransferase from Bacillus subtilis (183 aa), FASTA scores: E(): 0.0044, (26.4% identity in 110 aa overlap). P9WQG7 No intermediary metabolism and respiration 2VZY,2VZZ P9WQG7 GO:0008152,GO:0008080 P9WQG7 NC_000962.3 Mycobrowser_v5 CDS 1261922 1262158 . + 0 Rv1134 Rv1134 Unknown Hypothetical protein Rv1134, (MTCI65.01), len: 78 aa. Hypothetical unknown protein. O06534 No conserved hypotheticals O06534 NC_000962.3 Mycobrowser_v5 CDS 898081 898710 . + 0 Rv0804 Rv0804 Function unknown Conserved hypothetical protein Rv0804, (MTCY07H7A.05c), len: 209 aa. Conserved hypothetical protein, showing similarity with C-terminus of Rv1863c|MTCY359.10 conserved hypothetical protein from Mycobacterium tuberculosis (256 aa), FASTA scores: opt: 199, E(): 1.2e-05, (33.2% identity in 220 aa overlap); and Rv0658c. Contains PS01151 Fimbrial biogenesis outer membrane usher protein signature. I6Y4V2 No conserved hypotheticals O06630 I6Y4V2 GO:0016020 NC_000962.3 Mycobrowser_v5 CDS 898831 899787 . + 0 Rv0805 Rv0805 Hydrolyzes cyclic nucleotide monophosphate to nucleotide monophosphate. Shown to hydrolyze 2',3'-cNMP and 3',5'-cNMP. Class III cyclic nucleotide phosphodiesterase (cNMP PDE) Rv0805, (MTCY07H7A.04c), len: 318 aa. Cyclic nucleotide phosphodiesterase (cNMP PDE) (See Shenoy et al., 2005), member of binuclear metallophosphoesterase superfamily, equivalent to Q50024 from Mycobacterium leprae (317 aa), FASTA scores: opt: 1713, E(): 0, (82.5% identity in 315 aa overlap). Also shows similarity with hypothetical proteins and icc proteins e.g. SC9B1.22c|T35867 hypothetical protein from Streptomyces coelicolor (305 aa); P36650|ICC_ECOLI icc protein from Escherichia coli (275 aa), FASTA scores: opt: 310, E(): 8.9e-14, (31.3% identity in 214 aa overlap); etc. P9WP65 No intermediary metabolism and respiration 2HY1,2HYO,2HYP,3IB7,3IB8 O06629 P9WP65 GO:0016787 P9WP65 NC_000962.3 Mycobrowser_v5 CDS 908181 908483 . - 0 Rv0814c sseC2 Thought to be involved in sulphur metabolism. Conserved protein SseC2 Rv0814c, (MTV043.06c, O05794), len: 100 aa. SseC2, conserved protein, highly similar to AAA62972.1|U15182|MLU15182_17 hypothetical protein from Mycobacterium leprae (143 aa), FASTA scores: opt: 545, E(): 0, (84.0% identity in 100 aa overlap); and NP_302441.1|NC_002677|Z95150|MTCY164_29 conserved hypothetical protein from Mycobacterium leprae (100 aa), FASTA scores: opt: 647, E(): 0, (100.0% identity in 100 aa overlap). Also highly similar to M29612|SERCYSA_5 rhodanese-like protein from Saccharopolyspora erythraea (101 aa), FASTA scores: opt: 345, E(): 1.2e-18, (57.1% identity in 98 aa overlap); and similar at the C-terminus to the C-terminus of CAB94069.1|AL358692 conserved hypothetical protein from Streptomyces coelicolor (95 aa). Identical second copy present as Rv3118|MTCY164.28|SSEC1 from Mycobacterium tuberculosis (100 aa) (100.0% identity in 100 aa overlap). P0CG95 No intermediary metabolism and respiration Q7D986 P0CG95 NC_000962.3 Mycobrowser_v5 CDS 916477 917646 . - 0 Rv0823c Rv0823c Thought to be involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0823c, (MTV043.15c), len: 389 aa. Possible transcriptional regulator (resembles nitrogen regulation protein), equivalent (but longer 24 aa in N-terminus) to MLU15182_31|U15182|NtrB NtrB protein from Mycobacterium leprae (384 aa), FASTA scores: opt: 2070, E(): 0, (82.3% identity in 384 aa overlap) (see citation below). Also highly similar to CAB63312.1|AL133471|SCC82.03c hypothetical protein from Streptomyces coelicolor (406 aa); and to many transcriptional regulators members of UPF0034 family (NIFR3/SMM1) e.g. D26185|BAC180K_143 protein similar to transcriptional regulator (nitrogen regulation protein) from Bacillus subtilis (333 aa), FASTA scores: opt: 609, E(): 1.4e-32, (38.3% identity in 326 aa overlap); NP_349795.1|NC_003030 NifR3 family enzyme from Clostridium acetobutylicum (321 aa); etc. Contains PS01136 Uncharacterized protein family UPF0034 signature. P9WNS7 No regulatory proteins O53835 P9WNS7 GO:0055114,GO:0017150,GO:0008033,GO:0050660 1.-.-.- P9WNS7 NC_000962.3 Mycobrowser_v5 tRNA 924213 924286 . + 0 MTB000013 pheU - tRNA-Phe (GAA) pheU, tRNA-Phe, anticodon gaa, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 901635 902024 . + 0 Rv0807 Rv0807 Function unknown Conserved hypothetical protein Rv0807, (MTCY07H7A.02c), len: 129 aa. Conserved hypothetical protein, equivalent to O05761|MLCB5_31 hypothetical 14.0 kDa protein from Mycobacterium leprae (131 aa), FASTA scores: E(): 0, (73.4% identity in 128 aa overlap). Also highly similar to BAA89438.1|AB003158|ORF3 hypothetical protein from Corynebacterium ammoniagenes (132 aa); and C-terminus of SCD25.20|CAB56364.1|AL118514 hypothetical protein from Streptomyces coelicolor (202 aa). I6Y8U3 No conserved hypotheticals I6Y8U3 I6Y8U3 NC_000962.3 Mycobrowser_v5 CDS 907338 908018 . - 0 Rv0813c Rv0813c Function unknown Conserved protein Rv0813c, (MTV043.05c), len: 226 aa. Conserved protein, highly similar to U15182|MLU15182_16 hypothetical protein from Mycobacterium leprae (242 aa), FASTA scores: opt: 1191, E(): 0, (78.3% identity in 226 aa overlap); and NP_302442.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (228 aa). Also similar to AB94083.1|AL358692|SCD66.16 hypothetical protein from Streptomyces coelicolor (191 aa); and Rv2717c|MTCY05A6_37 hypothetical protein from Mycobacterium tuberculosis (164 aa), FASTA score: (30.4% identity in 171 aa overlap). Possibly a new bacterial family of fatty acid-binding protein-like proteins (See Shepard et al., 2007). P9WFG9 No conserved hypotheticals 2FWV O53827 P9WFG9 GO:0006810 P9WFG9 NC_000962.3 Mycobrowser_v5 CDS 917734 918750 . - 0 Rv0824c desA1 Catalyzes the principal conversion of saturated fatty acids to unsaturated fatty acids. Thought to convert stearoyl-ACP to oleoyl-ACP by introduction of a cis double bond between carbons delta-9 and delta-10 of the acyl chain [catalytic activity: stearoyl-[acyl-carrier protein] + AH2 + O2 = oleoyl-[acyl-carrier protein] + a + 2 H2O]. Probable acyl-[acyl-carrier protein] desaturase DesA1 (acyl-[ACP] desaturase) (stearoyl-ACP desaturase) (protein Des) Rv0824c, (MTV043.16c), len: 338 aa. Probable desA1 (alternate gene name: des), acyl-[acyl-carrier protein] desaturase (stearoyl-ACP desaturase) (see Jackson et al., 1997), equivalent to U15182|MLU15182_32 acyl-[ACP] desaturase from Mycobacterium leprae (338 aa), FASTA scores: opt: 1880, E(): 0, (79.9% identity in 338 aa overlap); and highly similar in part to fragment CAB96061.1|AJ250019 Steroyl-ACP-desaturase from Mycobacterium avium subsp. paratuberculosis (93 aa). Also similar to other fatty acid desaturases e.g. T35035 probable acyl-[acyl-carrier protein] desaturase from Streptomyces coelicolor (328 aa); Q40731|STAD_ORYSA acyl-[acyl-carrier protein] desaturase precursor from Oryza sativa (Rice) (390 aa); etc. Also highly similar to desA2|Rv1094 from Mycobacterium tuberculosis (275 aa). Contains PS00225 Crystallins beta and gamma 'Greek key' motif signature. Belongs to the fatty acid desaturase family. Cofactor: ferredoxin, ferredoxin NADPH reductase, and NADPH. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WNZ7 No lipid metabolism Q50824 P9WNZ7 GO:0006633,GO:0055114,GO:0046914,GO:0045300 1.14.19.- P9WNZ7 NC_000962.3 Mycobrowser_v5 CDS 937593 937835 . + 0 Rv0841 Rv0841 Unknown Probable conserved transmembrane protein Rv0841, len: 80 aa. Conserved transmembrane protein, highly similar to C-terminus of next ORF Rv0842|O53854 putative membrane protein from Mycobacterium tuberculosis (442 aa), FASTA scores: opt: 246, E(): 3.3e-10, (59.7% identity in 72 aa overlap). Replace previous Rv0841c. I6WZK3 No cell wall and cell processes I6WZK3 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1143736 1144467 . + 0 Rv1022 lpqU Unknown Probable conserved lipoprotein LpqU Rv1022, (MTCY10G2.27c), len: 243 aa. Probable lpqU conserved lipoprotein. Similar to Mycobacterium tuberculosis hypothetical protein Rv1230c|MTV006.02C, FASTA scores: E(): 2.8e-18, (37.9% identity in 240 aa overlap). Similar to AL133423|SC4A7.37 hypothetical protein from Streptomyces coelicolor (421 aa), FASTA scores: opt: 474, E(): 2.7e-21, (42.2% identity in 211 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. P96378 No cell wall and cell processes P96378 P96378 NC_000962.3 Mycobrowser_v5 CDS 1279998 1280846 . - 0 Rv1153c omt Function unknown, but supposedly involved in lipid metabolism Probable O-methyltransferase Omt Rv1153c, (MTCI65.20c), len: 282 aa. Probable omt, O-methyltransferase, similar to TCMP_STRGA|P39887 Tetracenomycin polyketide synthesis O-methyltransferase tcmP from Streptomyces glaucescens (270 aa), FASTA scores: opt: 368, E(): 1.7e-17, (31.3% identity in 233 aa overlap). O06551 No lipid metabolism O06551 O06551 GO:0008168 2.1.1.- O06551 NC_000962.3 Mycobrowser_v5 CDS 1402778 1403386 . - 0 Rv1255c Rv1255c Involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv1255c, (MTCY50.27), len: 202 aa. Possible regulatory protein, similar to others e.g. ACRR_ECOLI|P34000 potential acrab operon repressor from E. coli (215 aa), FASTA scores: opt: 128, E(): 0.25, (42.1% identity in 57 aa overlap). Helix turn helix motif present at aa 36-57 (+5.48 SD). P9WMD5 No regulatory proteins Q11063 P9WMD5 GO:0006350,GO:0003700,GO:0006355 P9WMD5 NC_000962.3 Mycobrowser_v5 ncRNA 918264 918458 . + 0 MTB000053 ASdes Unknown Putative small regulatory RNA ASdes, putative small regulatory RNA (See Arnvig and Young, 2009). Alternate 5'-ends at positions 918350 and 918365. Alternate 3'-ends at positions 918432 and 918412. No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 903725 904819 . + 0 Rv0809 purM Involved in de novo purine biosynthesis (at the fifth step) [catalytic activity: ATP + 5'-phosphoribosylformylglycinamidine = ADP + phosphate + 5'-phosphoribosyl-5-aminoimidazole]. Probable phosphoribosylformylglycinamidine CYCLO-ligase PurM (AIRS) (phosphoribosyl-aminoimidazole synthetase) (air synthase) Rv0809, (MTV043.01), len: 364 aa. Probable purM, 5'-phosphoribosyl-5-aminoimidazole synthetase, equivalent to NP_302446.1|NC_002677 5'-phosphoribosyl-5-aminoimidazole synthase from Mycobacterium leprae (364 aa). Also highly similar to many e.g. P12043|PUR5_BACSU phosphoribosylformylglycinamidine CYCLO-ligase from Bacillus subtilis (346 aa), FASTA scores: opt: 1023, E(): 0, (46.5% identity in 331 aa overlap); U68765|STU68765_2 from Salmonella typhimurium (345 aa), FASTA scores: opt: 1014, E():0, (47.6% identity in 330 aa overlap); etc. I6Y4V6 No intermediary metabolism and respiration O53823 I6Y4V6 GO:0005524,GO:0005737,GO:0004641,GO:0006189 6.3.3.1 I6Y4V6 NC_000962.3 Mycobrowser_v5 CDS 904905 905087 . - 0 Rv0810c Rv0810c Function unknown Conserved hypothetical protein Rv0810c, (MTV043.02c), len: 60 aa. Conserved hypothetical protein, with its N-terminus highly similar to NP_302445.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (62 aa); and AL118514|SCD25_24 hypothetical protein from Streptomyces coelicolor (84 aa), FASTA scores: opt: 180, E(): 5.7e-07, (51.8% identity in 56 aa overlap). I6XWB9 No conserved hypotheticals O53824 I6XWB9 NC_000962.3 Mycobrowser_v5 CDS 905234 906340 . - 0 Rv0811c Rv0811c Function unknown Conserved protein Rv0811c, (MTV043.03c), len: 368 aa. Conserved protein, equivalent to U2266F|U15182|MLU15182_13 hypothetical protein from Mycobacterium leprae (366 aa), FASTA scores: opt: 1870, E(): 0, (77.4% identity in 367 aa overlap). Also highly similar to BAA89441.1|AB003158|ORF4 hypothetical protein from Corynebacterium ammoniagenes (359 aa); and CAB94085.1|AL358692 conserved hypothetical protein from Streptomyces coelicolor (321 aa). I6X9V3 No conserved hypotheticals O53825 I6X9V3 GO:0005737,GO:0006546,GO:0004047 I6X9V3 NC_000962.3 Mycobrowser_v5 CDS 1074440 1075114 . - 0 Rv0962c lprP Unknown Possible lipoprotein LprP Rv0962c, (MTCY10D7.12), len: 224 aa. Possible lprP, lipoprotein. Contains possible N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. P9WK39 No cell wall and cell processes P9WK39 GO:0005886 P9WK39 NC_000962.3 Mycobrowser_v5 CDS 906423 907292 . + 0 Rv0812 Rv0812 Acts on amino acids. Probable amino acid aminotransferase Rv0812, (MTV043.04), len: 289 aa. Probable amino acid aminotransferase, similar to other amino acid aminotransferases, generelly class-IV of pyridoxal-phosphate-dependent aminotransferases, and especially ILVE proteins and PABC proteins e.g. B76065.1|AL157953 putative aminotransferase from Streptomyces coelicolor (273 aa); NP_069766.1|NC_000917 branched-chain amino acid aminotransferase (ilvE) from Archaeoglobus fulgidus (290 aa); P54692|DAAA_BACLI D-alanine aminotransferase from Bacillus licheniformis (283 aa); P28305|PABC_ECOLI|B1096 4-amino-4-deoxychorismate lyase (ADC lyase) From Escherichia coli strain K12 (269 aa), FASTA scores: opt: 165, E(): 0.00064, (26.8% identity in 198 aa overlap); etc. Note that previously known as pabC. Q79FW0 No intermediary metabolism and respiration Q79FW0 Q79FW0 GO:0008483,GO:0008152 2.6.1.- Q79FW0 NC_000962.3 Mycobrowser_v5 CDS 910030 910842 . - 0 Rv0817c Rv0817c Unknown Probable conserved exported protein Rv0817c, (MTV043.09c), len: 270 aa. Probable conserved exported protein, with N-terminal signal sequence, equivalent (but shorter 13 aa) to U15182|MLU15182_22|U2266M probable exported protein from Mycobacterium leprae (283 aa), FASTA scores: opt: 1287, E(): 0, (73.0% identity in 270 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). I6WZH9 No cell wall and cell processes I6WZH9 NC_000962.3 Mycobrowser_v5 CDS 1308223 1308792 . - 0 Rv1176c Rv1176c Function unknown Conserved hypothetical protein Rv1176c, (MTV005.12c), len: 189 aa. Conserved hypothetical protein, some similarity to P94443|D78508 hypothetical protein from Bacillus subtilis (182 aa), FASTA scores: opt: 219, E(): 1.7e-15, (25.1% identity in 183 aa overlap). Similar to Mycobacterium tuberculosis hypothetical protein Rv0047c. O50432 No conserved hypotheticals O50432 O50432 O50432 NC_000962.3 Mycobrowser_v5 CDS 911736 912683 . + 0 Rv0819 mshD Involved in the fourth step of mycothiol biosynthesis GCN5-related N-acetyltransferase, MshD Rv0819, (MTV043.11), len: 315 aa. MshD, acetyltransferase involved in mycothiol synthesis (see Koledin et al., 2002). Contains two GNAT (Gcn5-related N-acetyltransferase) domains. See Vetting et al. 2003, 2005, 2006. Equivalent to U2266N|U15182|MLU15182_24 hypothetical protein from Mycobacterium leprae (312 aa), FASTA scores: opt: 1540, E(): 0, (75.2% identity in 314 aa overlap). Also highly similar to CAB88484.1|AL353816 putative acetyltransferase from Streptomyces coelicolor (309 aa). P9WJM7 No intermediary metabolism and respiration 1OZP,1P0H,2C27 O53831 P9WJM7 GO:0008152,GO:0008080 P9WJM7 NC_000962.3 Mycobrowser_v5 CDS 912726 913502 . + 0 Rv0820 phoT Involved in active transport of inorganic phosphate across the membrane (import); responsible for energy coupling to the transport system. This is one of the proteins required for binding-protein-mediated phosphate transport. Probable phosphate-transport ATP-binding protein ABC transporter PhoT Rv0820, (MTV043.12), len: 258 aa. Probable phoT, phosphate-transport ATP-binding protein ABC transporter (see citation below), equivalent to PhoT|MLU15182_28|U15182 phosphate transport system ABC transporter from Mycobacterium leprae (258 aa), FASTA scores: opt: 1556, E(): 0, (91.5% identity in 258 aa overlap). Also highly similar to others e.g. CAB88472.1|AL353816 phosphate ABC transport system ATP-binding protein from Streptomyces coelicolor (258 aa); etc. Note that also highly similar to many PstB proteins e.g. AAC15686.1|AF045938|PstB putative ABC transporter nucleotide binding subunit from Mycobacterium smegmatis (258 aa). Contains PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters). P9WQL1 No cell wall and cell processes O53832 P9WQL1 GO:0005315,GO:0015415,GO:0006817,GO:0005886,GO:0005524 3.6.3.27 P9WQL1 NC_000962.3 Mycobrowser_v5 CDS 918912 919553 . - 0 Rv0825c Rv0825c Function unknown Conserved protein Rv0825c, (MTV043.17c), len: 213 aa. Conserved protein, highly similar, but in part (between aa ~43-96) to fadD27|Rv0275c|MTV035.03 putative fatty-acid-CoA ligase from Mycobacterium tuberculosis (241 aa), FASTA scores: E(): 7.3e-09, (32.6% identity in 190 aa overlap). Also shows similarity with other proteins from Mycobacterium tuberculosis e.g. Rv0078|AL0214|MTV030_22 (201 aa), FASTA scores: opt:118, E(): 0.32, (34.5% identity in 113 aa overlap); etc. O53836 No conserved hypotheticals O53836 O53836 GO:0006350,GO:0003700,GO:0006355 O53836 NC_000962.3 Mycobrowser_v5 CDS 919634 920689 . + 0 Rv0826 Rv0826 Function unknown Conserved hypothetical protein Rv0826, (MTV043.18), len: 351 aa. Conserved hypothetical protein, similar to CAB94053.1|AL358672|SC7A12.06 hypothetical protein from Streptomyces coelicolor (300 aa); and NP_421372.1|NC_002696 hypothetical protein from Caulobacter crescentus (299 aa). Also similar to other proteins from Mycobacterium tuberculosis e.g. Rv1645c|Z85982|MTCY06H11.09 (351 aa), FASTA scores: opt: 1199, E(): 0, (57.5% identity in 299 aa overlap); Rv2237; Rv0276; etc. O53837 No conserved hypotheticals O53837 O53837 NC_000962.3 Mycobrowser_v5 CDS 973806 974294 . - 0 Rv0875c Rv0875c Unknown Possible conserved exported protein Rv0875c, (MTCY31.03c), len: 162 aa. Possible conserved exported protein, equivalent to MLCB57_11|O33056 possible exported protein from Mycobacterium leprae (162 aa), FASTA scores: opt: 789, E(): 0, (71.4% identity in 161 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). P9WKR7 No cell wall and cell processes P9WKR7 NC_000962.3 Mycobrowser_v5 CDS 920741 921133 . - 0 Rv0827c kmtR Represses transcription from the KMTR operator-promoter. Repression is alleviated by NI(II) or cobalt(II). Metal sensor transcriptional regulator KmtR (ArsR-SmtB family) Rv0827c, (MTV043.19c), len: 130 aa. KmtR, transcriptional regulator (See Campbell et al., 2007), similar to many e.g. CAC42856.1|AL592292 putative regulatory protein from Streptomyces coelicolor (115 aa); NP_301626.1|NC_002677 putative ArsR-family transcriptional regulator from Mycobacterium leprae (140 aa); BSUB0011_75|O31844|Z99114 YOZA protein from Bacillus subtilis (107 aa), FASTA scores: opt: 208, E(): 3.2e-08, (35.5% identity in 93 aa overlap); etc. Also similar to MTCY27.22c|Z95208 from Mycobacterium tuberculosis (135 aa), FASTA scores: opt: 201, E(): 1.2e-07, (35.7% identity in 98 aa overlap). Contains probable helix-turn helix motif from aa 42-63 (Score 1300, +3.61 SD). Belongs to the ArsR family of transcriptional regulators. O53838 No regulatory proteins O53838 O53838 GO:0006355,GO:0006350,GO:0003700,GO:0005622 O53838 NC_000962.3 Mycobrowser_v5 CDS 924951 925364 . + 0 Rv0832 PE_PGRS12 Function unknown PE-PGRS family protein PE_PGRS12 Rv0832, (MTV043.24), len: 137 aa. PE_PGRS12, Member of the Mycobacterium tuberculosis PE family, possibly PGRS subfamily of gly-rich proteins (see citation below), highly similar to many others e.g. MTCY1A11.25c|Z78020 (498 aa), FASTA scores: opt: 529, E(): 5.2e-22, (61.8% identity in 136 aa overlap); etc. Appears to have incurred frameshift as next ORF should be continuation; sequence has been checked but no error found. Q79FV8 No PE/PPE Q79FV8 Q79FV8 Q79FV8 NC_000962.3 Mycobrowser_v5 CDS 921191 921613 . - 0 Rv0828c Rv0828c Function unknown; probably involved in deamination of specific substrate. Possible deaminase Rv0828c, (MTV043.20c), len: 140 aa. Possible deaminase, with its N-terminus highly similar to middle part of NP_302602.1|NC_002677 possible cytidine/deoxycytidylate deaminase from Mycobacterium leprae (171 aa). Also similar to other deaminases e.g. CAC18715.2|AL451182 putative deaminase from Streptomyces coelicolor (167 aa); NP_251189.1|NC_002516 probable deaminase from Pseudomonas aeruginosa (151 aa); NP_108387.1|NC_002678 nitrogen fixation protein gene from Mesorhizobium loti (149 aa); etc. Also similar to many conserved hypothetical proteins e.g. NP_389200.1|NC_000964 hypothetical protein from Bacillus subtilis (156 aa), FASTA scores: E(): 1.3e-07, (38.9% identity in 95 aa overlap); etc. And similar to Rv3752c possible deaminase from Mycobacterium tuberculosis. Contains PS00903 Cytidine and deoxycytidylate deaminases zinc-binding region signature. Belongs to the cytidine and deoxycytidylate deaminases family. O53839 No intermediary metabolism and respiration O53839 O53839 GO:0008270,GO:0016787 3.5.-.- O53839 NC_000962.3 Mycobrowser_v5 CDS 921575 921865 . + 0 Rv0829 Rv0829 Required for the transposition of the insertion element IS1605'. Possible transposase (fragment) Rv0829, (MTV043.21), len: 96 aa. Possible transposase for IS1605' (fragment), similar to C-terminal end of many mycobacterial transposases and hypothetical proteins e.g. Z74024|MTCY274_16 from Mycobacterium tuberculosis (460 aa), FASTA scores: opt: 668, E(): 6.2e-32, (98.9% identity in 93 aa overlap); MTV002_57|O33333 transposase from Mycobacterium tuberculosis ; L07627|SERRY1_1 insertion element IS1136 from Saccharopolyspora erythraea (90 aa), FASTA score: (34.9% identity in 83 aa overlap). O53840 No insertion seqs and phages O53840 O53840 NC_000962.3 Mycobrowser_v5 CDS 921970 922875 . + 0 Rv0830 Rv0830 Possible methyltransferase Possible S-adenosylmethionine-dependent methyltransferase Rv0830, (MTV043.22), len: 301 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), member of Mycobacterium tuberculosis protein family consisting of the proteins Rv0726c, Rv0731c, Rv3399, Rv1729c|Z81360|MTCY4C12_14c (312 aa), FASTA scores: opt: 1014, E(): 0, (54.1% identity in 292 aa overlap); etc. P9WFI3 No lipid metabolism O53841 P9WFI3 GO:0008168 2.1.1.- P9WFI3 NC_000962.3 Mycobrowser_v5 CDS 939388 940392 . + 0 Rv0843 Rv0843 Function unknown; probably involved in cellular metabolism. Probable dehydrogenase Rv0843, (MTV043.36), len: 334 aa. Probable dehydrogenase, similar to various dehydrogenases e.g. Q46142|Q46142 TPP-dependent acetoin dehydrogenase (326 aa), FASTA scores: opt: 500, E(): 2.4e-26, (32.3% identity in 300 aa overlap); P51267|ODPA_PORPU pyruvate dehydrogenase E1 component from Porphyra purpurea (344 aa), FASTA scores: opt: 451, E(): 4.7e-23, (29.6% identity in 311 aa overlap); etc. Also similar to Rv2497c|pdhA pyruvate dehydrogenase E1 component from Mycobacterium tuberculosis (367 aa). I6XWE5 No intermediary metabolism and respiration O53855 I6XWE5 GO:0016624,GO:0055114 1.-.-.- I6XWE5 NC_000962.3 Mycobrowser_v5 CDS 1184883 1185740 . + 0 Rv1062 Rv1062 Function unknown Conserved hypothetical protein Rv1062, (MTV017.15), len: 285 aa. Conserved hypothetical protein, some similarity to AL079356|SC6G9_10 hypothetical protein in Streptomyces coelicolor (289 aa), FASTA scores: opt: 556, E(): 1.2e-27, (39.0% identity in 287 aa overlap), and Z99111|BSUB0008_176 Bacillus subtilis (260aa), FASTA scores: opt: 163, E(): 0.0013, (27.4% identity in 179aa overlap). O53410 No conserved hypotheticals O53410 O53410 GO:0006629 O53410 NC_000962.3 Mycobrowser_v5 CDS 934720 935490 . + 0 Rv0838 lpqR Unknown Probable conserved lipoprotein LpqR Rv0838, (MTV043.31), len: 256 aa. Probable lpqR, conserved lipoprotein. Similar (except in N-terminus) to hypothetical proteins and D-alanyl-D-alanine dipeptidases e.g. NP_416005.1|NC_000913 hypothetical protein from Escherichia coli strain K12 (193 aa); NP_421076.1|NC_002696 D-alanyl-D-alanine dipeptidase from Caulobacter crescentus (212 aa); Q06241|VANX_ENTFC D-alanyl-D-alanine dipeptidase from Enterococcus faecium (202 aa), FASTA scores: opt: 198, E(): 1.9e-05, (28.1% identity in 199 aa overlap); etc. Contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O53850 No cell wall and cell processes O53850 O53850 GO:0016805,GO:0008237,GO:0006508,GO:0005618 O53850 NC_000962.3 Mycobrowser_v5 CDS 935577 936389 . + 0 Rv0839 Rv0839 Function unknown Conserved hypothetical protein Rv0839, (MTV043.32), len: 270 aa. Conserved hypothetical protein, similar to various hypothetical proteins or methyltransferases from yeast and bacteria e.g. T34740|SC1E6.19c|AL033505|SC1E6_19 hypothetical protein from Streptomyces coelicolor (273 aa), FASTA scores: opt: 1102, E(): 0, (58.6% identity in 263 aa overlap); T38024|Z98598|SPAC1B3.06c hypothetical protein from Schizosaccharomyces pombe (278 aa), FASTA scores: opt: 562, E(): 1.9e-3, (36.4% identity in 269 aa overlap); JC6531 avermectin B 5-O-methyltransferase from Streptomyces avermitilis (283 aa); etc. Also similar to other Mycobacterium tuberculosis hypothetical proteins that may be methyltransferases e.g. Rv1523, Rv2952, Rv1405c, etc. I6X9X6 No conserved hypotheticals O53851 I6X9X6 GO:0008168,GO:0008152 I6X9X6 NC_000962.3 Mycobrowser_v5 CDS 936457 937317 . - 0 Rv0840c pip Specifically catalyzes the removal of N-terminal proline residues from peptides. Thought to release the N-terminal proline from the dipeptides, pro-pro, pro-GLN, pro-TRP and pro-TYR; also from amides (pro-beta NA) and oligopeptides, pro-LEU-GLYNH2, pro-LEU-GLY and pro-PHE-GLY-LYS. Higher activity toward small peptides (up to three residues), but very low activity for longer peptides [catalytic activity: release of a N-terminal proline from a peptide]. Probable proline iminopeptidase Pip (prolyl aminopeptidase) (pap) Rv0840c, (MTV043.33c), len: 286 aa. Possible pip, proline iminopeptidase, similar to many e.g. P46541|PIP_BACCO proline iminopeptidase from bacillus coagulans (288 aa), FASTA scores: opt: 657, E(): 0, (37.6% identity in 282 aa overlap); NP_386922.1|NC_003047 putative proline iminopeptidase protein from Sinorhizobium meliloti (296 aa); etc. Belongs to peptidase family S33. I6Y8X0 No intermediary metabolism and respiration O53852 I6Y8X0 GO:0005737,GO:0006508,GO:0004177 3.4.11.5 I6Y8X0 NC_000962.3 Mycobrowser_v5 CDS 938112 939404 . + 0 Rv0842 Rv0842 Unknown Probable conserved integral membrane protein Rv0842, (MT0864, MTV043.35), len: 430 aa. Probable conserved integral membrane protein, showing similarity with other integral membrane proteins e.g. P28246|BCR_ECOLI bicyclomycin resistance protein from EScherichia coli (396 aa), FASTA scores: opt: 216, E(): 5.4e-07, (23.7% identity in 376 aa overlap); etc. O53854 No cell wall and cell processes O53854 O53854 GO:0055085 NC_000962.3 Mycobrowser_v5 CDS 4373726 4374013 . - 0 Rv3890c esxC Unknown ESAT-6 like protein EsxC (ESAT-6 like protein 11) Rv3890c, (MT4005, MTCY15F10.22), len: 95 aa. EsxC, ESAT-6 like protein (see Gey Van Pittius et al., 2001), equivalent to Q9K548|ES6B_MYCPA putative ESAT-6 like protein 11 (ORF3890C) from Mycobacterium paratuberculosis (95 aa), FASTA scores: opt: 490, E(): 3.3e-26, (76.85% identity in 95 aa overlap). Belongs to the ESAT6 family. P9WNI1 No cell wall and cell processes P9WNI1 NC_000962.3 Mycobrowser_v5 CDS 942680 944194 . - 0 Rv0846c Rv0846c May have multicopper oxidase activity. Probable oxidase Rv0846c, (MTV043.39c), len: 504 aa. Probable oxidase, showing similarity with several oxidases, mainly L-ascorbate oxidases and copper resistance proteins a (precursors) e.g. P24792|ASO_CUCMA L-ascorbate oxidase precursor (ascorbase) from Cucurbita maxima (Pumpkin) (Winter squash) (579 aa), FASTA scores: opt: 423, E(): 5.8e-18, (28.4% identity in 493 aa overlap); AF010496|AF010496_32 potential multicopper oxidase from Rhodobacter capsulatus (491 aa), FASTA scores: opt: 490, E(): 2.7e-22, (28.8% identity in 510 aa overlap); 47452|PCOA_ECOLI copper resistance protein A precursor (belongs to the family of multicopper oxidases) from Escherichia coli strain K12 (605 aa); etc. Contains PS00080 Multicopper oxidases signature 2 at C-terminus. Seems to belong to the family of multicopper oxidases. I6WZK7 No intermediary metabolism and respiration O53858 I6WZK7 GO:0055114,GO:0016491,GO:0005507 1.-.-.- I6WZK7 NC_000962.3 Mycobrowser_v5 CDS 944343 944735 . + 0 Rv0847 lpqS Unknown Probable lipoprotein LpqS Rv0847, (MTV043.40), len: 130 aa. Probable lpqS, lipoprotein. Contains possible signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O53859 No cell wall and cell processes O53859 NC_000962.3 Mycobrowser_v5 CDS 948559 949395 . + 0 Rv0852 fadD16 Function unknown, but involved in lipid degradation. Possible fatty-acid-CoA ligase FadD16 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv0852, (MTV043.45), len: 278 aa. Possible fadD16, fatty-acid-CoA synthetase, similar in part to various CoA ligases e.g. P18163|LCFB_RAT long-chain-fatty-acid--CoA ligase from Rattus norvegicus (Rat) (699 aa); D49366|LEP4CCOALA_1 4-coumarate:CoA ligase from Lithospermum erythrorhizon (636 aa), FASTA scores: opt: 134, E(): 0.15, (26.8% identity in 213 aa overlap); orgp|L09229|HUMFACAL_1 long-chain acyl-coenzyme A from homo sapiens (human) (699 aa), FASTA score: (50.0% identity in 40 aa overlap); etc. Contains PS00626 Regulator of chromosome condensation (RCC1) signature 2. I6Y4Z4 No lipid metabolism I6Y4Z4 GO:0016874 6.2.1.- I6Y4Z4 NC_000962.3 Mycobrowser_v5 CDS 951183 951626 . + 0 Rv0854 Rv0854 Function unknown Conserved protein Rv0854, (MTV043.47), len: 147 aa. Conserved protein, similar to several hypothetical protein from Mycobacterium leprae e.g. NP_301674.1|NC_002677 (144 aa); NP_302683.1|NC_002677|Z95398|MLCL622.27c (156 aa), FASTA scores: opt: 193, E(): 1.6e-06, (24.6% identity in 134 aa overlap); NP_301218.1|NC_002677 (146 aa); MTCI28.04|Z97050 (184 aa), FASTA scores: opt: 171, E(): 5.8e-05, (21.5% identity in 135 aa overlap). Also similar to SC6G10.02c|T35511|AL049497|SC6G10_2 hypothetical protein from Streptomyces coelicolor (144 aa), FASTA scores: opt: 344, E(): 6.1e- 17, (37.6% identity in 141 aa overlap). And similar to many proteins from Mycobacterium tuberculosis e.g. downstreams ORFs Rv0856 and Rv0857, etc. I6X9Y7 No conserved hypotheticals O53866 I6X9Y7 I6X9Y7 NC_000962.3 Mycobrowser_v5 CDS 1008944 1010062 . + 0 Rv0906 Rv0906 Function unknown Conserved protein Rv0906, (MTCY31.34), len: 372 aa. Conserved protein, highly similar to others e.g. SC6A5.25|AL049485|T35416 hypothetical protein from Streptomyces coelicolor (370 aa), FASTA scores: opt: 1125, E(): 0, (51.3% identity in 335 aa overlap); NP_242955.1|NC_002570|BH2089 conserved protein from Bacillus halodurans (370 aa); etc. Also shows some similarity to C-terminus of Q48412|ROMA_KLEPN Q48412 outer membrane protein roma (fragment) from Klebsiella pneumoniae (132 aa), FASTA scores: opt: 319, E(): 8.5e-14, (46.2% identity in 104 aa overlap); NP_105215.1|NC_002678 hypothetical protein which contains similarity to outer membrane protein romA from Enterobacter cloacae (350 aa); etc. Predicted to be an outer membrane protein (See Song et al., 2008). P9WKP3 No conserved hypotheticals P9WKP3 P9WKP3 NC_000962.3 Mycobrowser_v5 CDS 1002441 1002704 . - 0 Rv0898c Rv0898c Function unknown Conserved hypothetical protein Rv0898c, (MTCY31.26c), len: 87 aa. Conserved hypothetical protein, highly similar to CAC01589.1|AL391041 hypothetical protein from Streptomyces coelicolor (87 aa). Also shows some similarity to Rv0709|MTCY210.28|rpmC from Mycobacterium tuberculosis (77 aa), FASTA score: (28.8% identity in 73 aa overlap). P9WKP5 No conserved hypotheticals P64753 P9WKP5 NC_000962.3 Mycobrowser_v5 CDS 946056 947315 . + 0 Rv0849 Rv0849 Thought to be involved in transport of undetermined substrate (possibly drug) across the membrane. Responsible for the translocation of the substrate across the membrane. Probable conserved integral membrane transport protein Rv0849, (MTV043.42), len: 419 aa. Probable conserved integral membrane transport protein, possibly member of major facilitator superfamily (MFS) involved in transport of drug, showing similarity with others e.g. T35055 probable transport system permease protein from Streptomyces coelicolor (436 aa); NP_295031.1|NC_001263 major facilitator family protein from Deinococcus radiodurans (458 aa); NP_455659.1|NC_003198 putative membrane transporter from Salmonella enterica subsp. enterica serovar Typhi (402 aa); etc. P9WJX5 No cell wall and cell processes O53861 P9WJX5 GO:0005886,GO:0055085,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 947312 947644 . + 0 Rv0850 Rv0850 Required for the transposition of an insertion element. Putative transposase (fragment) Rv0850, (MTV043.43), len: 110 aa. Putative transposase (fragment), similar in part to others e.g. Q45144|Q4514 transposable element IS31831 (436 aa), FASTA scores: opt: 175, E(): 4.3e-05, (38.6% identity in 57 aa overlap); etc. I6Y8X8 No insertion seqs and phages I6Y8X8 NC_000962.3 Mycobrowser_v5 CDS 949436 951118 . - 0 Rv0853c pdc Possible indole-3-pyruvate decarboxylase; EC 4.1.1.74 [catalytic activity: 3-(indol-3-YL)pyruvate = 2-(indol-3-YL)acetaldehyde + CO2], or possible pyruvate decarboxylase; EC 4.1.1.1 [catalytic activity: a 2-oxo acid = an aldehyde + CO2]. Probable pyruvate or indole-3-pyruvate decarboxylase Pdc Rv0853c, (MTV043.46c), len: 560 aa. Probable pdc, pyruvate or indole-pyruvate decarboxylase, equivalent to NP_302424.1|NC_002677 pyruvate (or indolepyruvate) decarboxylase from Mycobacterium leprae (569 aa). Also highly similar to others e.g. AAB06571.1|L80006 indolepyruvate decarboxylase from Pantoea agglomerans (550 aa); Q12629|DCPY_KLULA pyruvate decarboxylase from Kluyveromyces marxianus var. lactis (563 aa); P71323 indolepyruvate decarboxylase from Enterobacter herbicola (550 aa), FASTA scores: opt: 1642, E(): 0, (48.1% identity in 547 aa overlap); P23234|DCIP_ENTCL indole-3-pyruvate decarboxylase (indolepyruvate decarboxylase) from Enterobacter cloacae (552 aa), FASTA scores: opt: 1596, E(): 0, (46.8% identity in 551 aa overlap); etc. Contains PS00187 Thiamine pyrophosphate enzymes signature and PS00017 ATP/GTP-binding site motif A (P-loop). Cofactor: thiamine pyrophosphate. P9WG37 No intermediary metabolism and respiration O53865 P9WG37 GO:0000287,GO:0030976,GO:0016740,GO:0016831 4.1.1.- P9WG37 NC_000962.3 Mycobrowser_v5 CDS 972546 973706 . - 0 Rv0874c Rv0874c Function unknown Conserved hypothetical protein Rv0874c, (MTCY31.02c), len: 386 aa. Conserved hypothetical protein, highly similar in part to SPU62616_1 hypothetical protein from Synechococcus sp. (280 aa), FASTA scores: E(): 6.3e-26, (35.2% identity in 264 aa overlap); SYCSLLLH_102 from Synechocystis sp. (447 aa), FASTA scores: E(): 1.1e-18, (29.5% identity in 400 aa overlap). Also highly similar to Rv0628c|MTCY20H10_9 from Mycobacterium tuberculosis (383 aa), FASTA scores: E():0, (81.5% identity in 383 aa overlap). P9WKR9 No conserved hypotheticals P0A5D3 P9WKR9 GO:0005886,GO:0016021 P9WKR9 NC_000962.3 Mycobrowser_v5 CDS 952825 953229 . + 0 Rv0856 Rv0856 Function unknown Conserved hypothetical protein Rv0856, (MTV043.49), len: 134 aa. Conserved hypothetical protein, showing weak similarity with NP_301674.1| (NC_002677) conserved hypothetical protein from Mycobacterium leprae (144 aa); and SC6G10.02c|T35511 hypothetical protein from Streptomyces coelicolor (144 aa). Also highly similar to other proteins from Mycobacterium tuberculosis e.g. neighbouring ORF downstream Rv0857 conserved hypothetical protein (126 aa), FASTA scores: E(): 7.4e-27, (62.0% identity in 100 aa overlap); neighbouring ORF Rv0854|MTV043_47 conserved hypothetical protein (147 aa), FASTA scores: E(): 1.6e-15, (36.6% identity in 123 aa overlap), MTCI28.04|Z97050|MTCI28_4 (184 aa), FASTA scores: opt: 127, E(): 0.036, (26.0% identity in 127 aa overlap); and MLCL622.27c|Z95398 (156 aa), FASTA scores: opt: 123, E(): 0.06, (26.4% identity in 125 aa overlap). O53868 No conserved hypotheticals O53868 O53868 O53868 NC_000962.3 Mycobrowser_v5 CDS 953257 953730 . + 0 Rv0857 Rv0857 Function unknown Conserved hypothetical protein Rv0857, (MTV043.50), len: 157 aa. Conserved hypothetical protein, showing weak similarity with Q9X7Y8|SC6G10.02c|T35511 hypothetical protein from Streptomyces coelicolor (144 aa), FASTA scores: opt: 215, E(): 7.6e-08, (30.282% identity in 142 aa overlap). Also highly similar to other proteins from Mycobacterium tuberculosis e.g. upstream ORF Rv0856 (134 aa), FASTA scores: opt: 566, E(): 2e-32, (58.15% identity in 129 aa overlap); upstream ORF Rv0854 (147 aa), FASTA scores: opt: 401, E(): 7.2e-21, (41.8% identity in 146 aa overlap); MTCI28.04|Z97050 (184 aa), FASTA scores: opt: 122, E(): 0.031, (29.4% identity in 85 aa overlap); and MLCL622.27c|Z95398 (156 aa), FASTA scores: opt: 114, E(): 0.1, (30.9% identity in 55 aa overlap). Length extended since first submission (+33 aa). I6Y4Z9 No conserved hypotheticals O53869 I6Y4Z9 I6Y4Z9 NC_000962.3 Mycobrowser_v5 CDS 953727 954920 . - 0 Rv0858c dapC Involved in biosynthesis of diaminopimelate and lysine from aspartate semialdehyde (at the fourth step) [catalytic activity: L-glutamate + N-succinyl-2-amino-6-ketopimelate = 2-oxoglutarate + N-succinyl-L,L-2,6-diaminopimelate Probable N-succinyldiaminopimelate aminotransferase DapC (DAP-at) Rv0858c, (MTV043.51c), len: 397 aa. Probable dapC, N-succinyldiaminopimelate aminotransferase, highly similar to others from Eukaryota and bacteria, especially aspartate aminotransferases (transaminases), e.g. NP_177890.1|NC_003070 putative aminotransferase from Arabidopsis thaliana (440 aa); NP_419555.1|NC_002696 aminotransferase class I from Caulobacter crescentus (385 aa); NP_415133.1|NC_000913|AE0001|ECAE000165_8 putative aminotransferase from Escherichia coli strain K12 (386 aa), FASTA scores: opt: 830, E(): 0, (38.0% identity in 389 aa overlap); X99521|TAX99521_1 aspartate aminotransferase from Thermus aquaticus (383 aa), FASTA scores: opt: 702, E(): 0, (34.9% identity in 393 aa overlap); etc. Also similar to other putative aminotransferases from Mycobacterium tuberculosis e.g. Rv2294, Rv3565, etc. P9WPZ5 No intermediary metabolism and respiration 2O0R O53870 P9WPZ5 GO:0009058,GO:0030170,GO:0008483,GO:0016847 2.6.1.- P9WPZ5 NC_000962.3 Mycobrowser_v5 CDS 1145858 1146544 . + 0 Rv1024 Rv1024 Unknown Possible conserved membrane protein Rv1024, (MTCY10G2.25c), len: 228 aa. Possible conserved membrane protein, with a hydrophobic region from aa 83-101. Equivalent to ML0256|NP_301311.1|NC_002677 possible conserved membrane protein from Mycobacterium leprae (227 aa), S&W scores: 178, E()= 2e-72, Identities: 145/203 (71%). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P96376 No cell wall and cell processes P96376 P96376 GO:0051301,GO:0016021,GO:0005886,GO:0007049 NC_000962.3 Mycobrowser_v5 CDS 1417658 1418356 . - 0 Rv1268c Rv1268c Unknown Hypothetical protein Rv1268c, (MTCY50.14), len: 232 aa. Hypothetical unknown protein, probably secreted protein : contains possible signal peptide sequence (score 7.9 at residue 28). Predicted to be an outer membrane protein (See Song et al., 2008). P9WM47 No conserved hypotheticals P9WM47 GO:0006508,GO:0004197 P9WM47 NC_000962.3 Mycobrowser_v5 CDS 967344 967733 . - 0 Rv0870c Rv0870c Unknown Possible conserved integral membrane protein Rv0870c, (MTV043.63c), len: 129 aa. Possible conserved integral membrane protein, highly similar to other membrane proteins: putative secreted proteins or hypothetical proteins e.g. CAC08263.1| AL392146 putative integral membrane protein from Streptomyces coelicolor (138 aa); NP_233433.1|NC_002506 conserved hypothetical protein from Vibrio cholerae (143 aa); NP_455572.1|NC_003198 putative membrane protein from Salmonella enterica subsp. enterica serovar Typhi (148 aa); P37065|YCCF_ECOLI hypothetical 16.3 kDa protein from Escherichia coli (148 aa), FASTA scores: opt: 183, E(): 1.9e-06, (36.6% identity in 134 aa overlap); etc. I6Y8Z3 No cell wall and cell processes O53882 I6Y8Z3 NC_000962.3 Mycobrowser_v5 CDS 967898 968305 . + 0 Rv0871 cspB Function unknown; thought to act in response to low temperature. Probable cold shock-like protein B CspB Rv0871, (MTV043.64), len: 135 aa. Probable cspB, cold shock-like protein B, equivalent to Z99494|MLCB57_7|MLCB57.11 probable cold shock protein from Mycobacterium leprae (136 aa), FASTA scores: opt: 787, E(): 0, (86.0% identity in 136 aa overlap). Also highly similar (but often longer than) to others e.g. CAB93399.1|AL357524 cold shock protein B from Streptomyces coelicolor (127 aa); Q45099|CSPD_BACCE cold shock-like protein CSPD from Bacillus cereus (66 aa); Y101 81|LLCSPB_1 cold shock protein from Lactococcus lactis (66 aa), FASTA scores: opt: 220, E(): 2.5e-07, (48.3% identity in 60 aa overlap); etc. Seems to belong to the cold-shock domain (CSD) family. I6WZM9 No virulence, detoxification, adaptation O53883 I6WZM9 GO:0005737,GO:0006355,GO:0003677 I6WZM9 NC_000962.3 Mycobrowser_v5 CDS 1138967 1142671 . + 0 Rv1020 mfd Involved in nucleotide excision repair. Necessary for strand-specific repair. A lesion in the template strand blocks the RNA polymerase complex (RNAP). The RNAP-DNA-RNA complex is specifically recognized by TRCF which releases RNAP and the truncated transcript; the TCRF may replace RNAP at the lesion site and then recruit the UVRA/B/C repair system. Probable transcription-repair coupling factor Mfd (TRCF) Rv1020, (MTCY10G2.29c), len: 1234 aa. Probable mfd (alternate gene name: trcF), transcription-repair coupling factor (see citation below), similar to many e.g. MFD_ECOLI|P30958 transcription-repair coupling factor from Escherichia coli (1148 aa), FASTA scores: opt: 1900, E(): 0, (37.9% identity in 1107 aa overlap); similar to M. tuberculosis Rv2973c and Rv1633. Contains PS00017 ATP/GTP-binding site motif A (P-loop). In the N-terminal section; belongs to the UVRB family. In the C-terminal section; belongs to the helicase family. RECG subfamily. P9WMQ5 No information pathways P64326 P9WMQ5 GO:0008026,GO:0006281,GO:0003684,GO:0006355,GO:0003700,GO:0005524 3.6.4.- P9WMQ5 NC_000962.3 Mycobrowser_v5 CDS 2340871 2341815 . + 0 Rv2083 Rv2083 Unknown Conserved hypothetical protein Rv2083, (MTCY49.22), len: 314 aa. Conserved hypothetical protein. Similar to many e.g. Mycobacterium tuberculosis Rv3898c (110 aa) and Rv3897c (210 aa). P9WLK3 No conserved hypotheticals P9WLK3 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 960342 962612 . - 0 Rv0862c Rv0862c Function unknown Conserved protein Rv0862c, (MTV043.55c), len: 756 aa. Conserved protein, equivalent to NP_302419.1|NC_002677 possible DNA-binding protein from Mycobacterium leprae (753 aa); and highly similar (except in C-terminus) to MLCB57.01|Z99494|T45333 hypothetical protein from Mycobacterium leprae (>577 aa, truncated), FASTA scores: opt: 3047, E(): 0, (78.9% identity in 578 aa overlap). Also similar in part to SCD12A.03c|AB93395.1|AL357524 hypothetical protein from Streptomyces coelicolor (867 aa). O53874 No conserved hypotheticals O53874 O53874 NC_000962.3 Mycobrowser_v5 CDS 962599 962880 . + 0 Rv0863 Rv0863 Function unknown Conserved hypothetical protein Rv0863, (MTV043.56), len: 93 aa. Conserved hypothetical protein, highly similar to NP_302418.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (74 aa). Also weakly similar in part to U82598|ECU82598_135 hypothetical protein from Escherichia coli, FASTA scores: (32.4% identity in 71 aa overlap); and M74011|YEPYSCOP_8 hypothetical protein from Yersinia enterocolitica (165 aa), FASTA scores: (38.6 identity in 57 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). I6XWF9 No conserved hypotheticals I6XWF9 I6XWF9 NC_000962.3 Mycobrowser_v5 CDS 974291 975937 . - 0 Rv0876c Rv0876c Unknown Possible conserved transmembrane protein Rv0876c, (MTCY31.04c), len: 548 aa. Possible conserved transmembrane protein, equivalent to MLCB57_12|O33057 possible membrane protein from Mycobacterium leprae (579 aa), FASTA scores: opt: 2850, E(): 0, (81.0% identity in 568 aa overlap). Also highly similar (except in N-terminus) to CAB93403.1|AL357524 putative integral membrane protein from Streptomyces coelicolor (463 aa). P9WKR5 No cell wall and cell processes Q10564 P9WKR5 GO:0005886,GO:0055085,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 978934 979365 . + 0 Rv0880 Rv0880 Thought to be involved in transcriptional mechanism. Possible transcriptional regulatory protein (possibly MarR-family) Rv0880, (MTCY31.08), len: 143 aa. Possible transcriptional regulator, MarR family, equivalent to MLCB57_15|O3306|NP_302411.1|NC_002677 putative MarR-family protein from Mycobacterium leprae (143 aa), FASTA scores: opt: 818, E(): 0, (89.5% identity in 143 aa overlap). Also similar to many others e.g. CAB93410.1|AL357524 putative marR-family protein from Streptomyces coelicolor (145 aa); NP_251757.1|NC_002516 probable transcriptional regulator from Pseudomonas aeruginosa (147 aa); etc. Also similar to Rv2327 from Mycobacterium tuberculosis (163 aa). P9WMF1 No regulatory proteins P67745 P9WMF1 GO:0006355,GO:0006350,GO:0003700,GO:0005622 P9WMF1 NC_000962.3 Mycobrowser_v5 CDS 964312 965535 . - 0 Rv0867c rpfA Unknown. May be promote the resuscitation and growth of dormant, nongrowing cell. Possible resuscitation-promoting factor RpfA Rv0867c, (MTV043.60c), len: 407 aa. Possible rpfA, resuscitation-promoting factor (see citation below). N-terminus highly similar to N-terminal part (1-125 aa) of Z99494|MLCB57_3|NP_302417.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (174 aa), FASTA scores: opt: 785, E(): 1.8e-18, (63.0% identity in 200 aa overlap); and highly similar to C-terminus of NP_301299.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (375 aa); and middle part of NP_302360.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (157 aa). N-terminus also highly similar in part of three secreted proteins from Streptomyces coelicolor e.g. CAC09538.1|AL442120 putative secreted protein (244 aa). Regions highly similar to CAB76321.1|AL158060 putative membrane protein from Streptomyces coelicolor (121 aa); and middle part of CAB09664.1|Z96935 rpf from Micrococcus luteus (220 aa). Also highly similar in part to four resuscitation-promoting factors from Mycobacterium tuberculosis: Rv2450 (172 aa), Rv1009 (362 aa), Rv1884c (176 aa), and Rv2389c (154 aa). Contains a probable secretory signal sequence in N-terminus. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WG31 No cell wall and cell processes O53879 P9WG31 GO:0005576 P9WG31 NC_000962.3 Mycobrowser_v5 CDS 965983 966261 . - 0 Rv0868c moaD2 Involved in molybdenum cofactor biosynthesis. Probable molybdenum cofactor biosynthesis protein D 2 MoaD2 (molybdopterin converting factor small subunit) (molybdopterin [MPT] converting factor, subunit 1) Rv0868c, (MTV043.61c), len: 92 aa. Probable moaD2, molybdenum cofactor biosynthesis protein (molybdopterin converting factor (subunit 1)), similar to CAB88494.1|AL353816 putative molybdopterin converting factor from Streptomyces coelicolor (84 aa); and weakly similar to others MoaD proteins e.g. Z99111|BSUB0008_103 from Bacillus subtilis (77 aa), FASTA scores: opt: 86, E(): 2.8, (22.9% identity in 83 aa overlap); etc. Also some similarity with Rv3112|MOAD1|MTCY164.22 putative molybdenum cofactor biosynthesis protein D from Mycobacterium tuberculosis (83 aa), FASTA scores: opt: 113, E(): 0.024, (31.3% identity in 83 aa overlap). I6XWG2 No intermediary metabolism and respiration O53880 I6XWG2 GO:0006790 I6XWG2 NC_000962.3 Mycobrowser_v5 CDS 1418579 1418953 . - 0 Rv1269c Rv1269c Unknown Conserved probable secreted protein Rv1269c, (MTCY50.13), len: 124 aa. Conserved probable exported protein with putative N-terminal signal sequence. Similar to Mycobacterium tuberculosis protein Rv1813c|Y0DU_MYCTU|Q50620 hypothetical protein cy1a11.30 (137 aa), FASTA scores: E(): 9e-21, (41.6% identity in 137 aa overlap). P9WM45 No cell wall and cell processes P9WM45 NC_000962.3 Mycobrowser_v5 CDS 968424 970244 . - 0 Rv0872c PE_PGRS15 Function unknown PE-PGRS family protein PE_PGRS15 Rv0872c, (MTV043.65c), len: 606 aa. PE_PGRS15, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002), similar to many e.g. MTCY24A1.04c|Z95207 (615 aa), FASTA scores: opt: 2636, E(): 0, (64.6% identity in 619 aa overlap); etc. Q79FV3 No PE/PPE Q79FV3 Q79FV3 Q79FV3 NC_000962.3 Mycobrowser_v5 CDS 970505 972457 . + 0 Rv0873 fadE10 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE10 Rv0873, (MTV043.66-MTCY31.01), len: 650 aa. Probable fadE10, acyl-CoA dehydrogenase, highly similar to many e.g. CAB91129.1|AL355913 putative acyl CoA dehydrogenase from Streptomyces coelicolor (658 aa); P50544|ACDV_MOUSE acyl-CoA dehydrogenase from Mus musculus (656 aa); D30647|RATVLCAD_1 very-long-chain Acyl-CoA dehydrogenase from Rattus norvegicus (655 aa), FASTA scores: opt: 675, E(): 0, (33.9% identity in 380 aa overlap); etc. P9WQF7 No lipid metabolism P63429 P9WQF7 GO:0003995,GO:0055114,GO:0050660 P9WQF7 NC_000962.3 Mycobrowser_v5 CDS 976075 976863 . + 0 Rv0877 Rv0877 Function unknown Conserved hypothetical protein Rv0877, (MTCY31.05), len: 262 aa. Conserved hypothetical protein, equivalent to MLCB57_13|O33058 conserved hypothetical protein from Mycobacterium leprae (269 aa), FASTA scores: E(): 0, (80.5% identity in 257 aa overlap). Also highly similar (except in C-terminus) to SCD12A.13|CAB93404.1|AL357524 hypothetical protein from Streptomyces coelicolor (308 aa). P9WKR3 No conserved hypotheticals P64733 P9WKR3 NC_000962.3 Mycobrowser_v5 CDS 1426164 1427417 . + 0 Rv1277 Rv1277 Function unknown Conserved hypothetical protein Rv1277, (MTCY50.05c), len: 417 aa. Conserved hypothetical protein, some similarity to 3914967|O68033|SBCD_RHOCA exonuclease SBCD homolog from Rhodobacter capsulatus (405 aa). May be sbcD protein (see Mizrahi & Andersen 1998) Q50699 No conserved hypotheticals Q50699 Q50699 GO:0004527 Q50699 NC_000962.3 Mycobrowser_v5 CDS 3127364 3128272 . - 0 Rv2820c Rv2820c Unknown Hypothetical protein Rv2820c, (MTCY16B7.22), len: 302 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WJF7 No conserved hypotheticals P9WJF7 P9WJF7 NC_000962.3 Mycobrowser_v5 CDS 980225 980509 . + 0 Rv0882 Rv0882 Unknown Probable transmembrane protein Rv0882, (MTCY31.10), len: 94 aa. Probable transmembrane protein. P9WKQ9 No cell wall and cell processes P9WKQ9 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 980506 981267 . - 0 Rv0883c Rv0883c Function unknown Conserved hypothetical protein Rv0883c, (MTCY31.11c), len: 253 aa. Conserved hypothetical protein, equivalent to O3306|MLCB57_16 conserved hypothetical protein from Mycobacterium leprae (251 aa), FASTA scores: E(): 0, (79.4% identity in 253 aa overlap). Also highly similar to N_terminus of AL009204|SC9B10_22 hypothetical protein from Streptomyces coelicolor (352 aa), FASTA scores: E(): 6.1e-20, (35.0% identity in 246 aa overlap). P9WKQ7 No conserved hypotheticals P64739 P9WKQ7 P9WKQ7 NC_000962.3 Mycobrowser_v5 CDS 982762 983784 . + 0 Rv0885 Rv0885 Function unknown Conserved hypothetical protein Rv0885, (MTCY31.13), len: 340 aa. Conserved hypothetical protein, equivalent to O33063|MLCB57_18 possible transmembrane protein from Mycobacterium leprae (341 aa), FASTA score: (83.9% identity in 341 aa overlap). Also similar except in C-terminus to T35630 probable membrane protein from Streptomyces coelicolor (312 aa). P9WKQ5 No conserved hypotheticals P9WKQ5 GO:0005886,GO:0016021 P9WKQ5 NC_000962.3 Mycobrowser_v5 CDS 983803 985530 . + 0 Rv0886 fprB Serves as the first electron transfer protein in all the P450 systems [catalytic activity: reduced adrenodoxin + NADP+ = oxidized adrenodoxin + NADPH]. Probable NADPH:adrenodoxin oxidoreductase FprB (adrenodoxin reductase) (AR) (ferredoxin-NADP(+) reductase) Rv0886, (MTCY31.14), len: 575 aa. Probable fprB, ferredoxin/ferredoxin-NADP(+) reductase (NADPH:adrenodoxin oxidoreductase), equivalent to O3306|MLCB57_19 ferredoxin/ferredoxin--NADP reductase from Mycobacterium leprae (555 aa), FASTA scores: E(): 0, (76.6 identity in 560 aa overlap). Also highly similar or similar to others e.g. NP_294219.1|NC_001263 putative ferredoxin/ferredoxin--NADP reductase from Deinococcus radiodurans (479 aa) (N-terminus shorter); P22570|ADRO_HUMAN NADPH:adrenodoxin oxidoreductase from homo sapiens (497 aa), FASTA scores: opt: 624, E(): 3e-30, (39.7% identity in 484 aa overlap); P08165|ADRO_BOVIN NADPH:adrenodoxin oxidoreductase from Bos taurus (492 aa); etc. Also similar to others from Mycobacterium tuberculosis e.g. Rv3106, Rv3858c, etc. Contains PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature. P9WJI1 No intermediary metabolism and respiration P65528 P9WJI1 GO:0009055,GO:0022900,GO:0004324,GO:0046872,GO:0006810,GO:0051539 1.18.1.2 P9WJI1 NC_000962.3 Mycobrowser_v5 CDS 987233 988705 . + 0 Rv0888 Rv0888 Unknown Probable exported protein Rv0888, (MTCY31.16), len: 490 aa. Probable exported protein. Equivalent to AAK45157.1 from Mycobacterium tuberculosis strain CDC1551 (507 aa) but shorter 17 aa. Contains possible N-terminal signal sequence. Predicted to be an outer membrane protein (See Song et al., 2008). P9WKQ1 No cell wall and cell processes P64743 P9WKQ1 GO:0005886,GO:0016021 P9WKQ1 NC_000962.3 Mycobrowser_v5 CDS 989948 992596 . - 0 Rv0890c Rv0890c Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably LuxR-family) Rv0890c, (MTCY31.18c), len: 882 aa. Probable transcriptional regulatory protein, LuxR family, highly similar (but shorter 238 aa in N-terminus) to NP_302202.1|NC_002677 possible transcriptional regulator from Mycobacterium leprae (1106 aa). Also highly similar (generally in part) to others e.g. T50568 probable multi-domain regulatory protein from Streptomyces coelicolor (1334 aa); P10957|NARL_ECOLI nitrate/nitrite response regulator protein from Escherichia coli (216 aa), FASTA scores: opt: 193, E(): 6e-06, (37.4% identity in 99 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. MTCY02B10_22, MTV008_44, MTV036_21, and MTCY31_24. Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00622 Bacterial regulatory proteins, luxR family signature, and probable helix-turn helix motif from aa 836 to 857 (Score 1559, +4.50 SD). Belongs to the LuxR/UhpA family of transcriptional regulators. Alternative nucleotide at position 990001 (G->C; P866A) has been observed. P9WMG1 No regulatory proteins Q10550 P9WMG1 GO:0005622,GO:0006355,GO:0043565,GO:0006350,GO:0003700,GO:0000156,GO:0000160,GO:0006952 P9WMG1 NC_000962.3 Mycobrowser_v5 CDS 992598 993455 . - 0 Rv0891c Rv0891c Thought to be involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0891c, (MTCY31.19c), len: 285 aa. Possible transcriptional regulator, highly similar in N-terminus to NP_302202.1|NC_002677 possible transcriptional regulator from Mycobacterium leprae (1106 aa). Also highly similar to several Mycobacterium tuberculosis putative transcriptional regulators e.g. Q1102|MTCY02B10_22 probable transcriptional regulatory protein (1159 aa), FASTA scores: opt: 702, E(): 8.3e-40, (50.6% identity in 247 aa overlap); MTV036_21; MTV008_44; MTCY02B10_23. Also shows similarity with several adenylate cyclases and hydrolases from other organisms. P9WMV1 No regulatory proteins Q10551 P9WMV1 GO:0016849,GO:0009190 P9WMV1 NC_000962.3 Mycobrowser_v5 CDS 993853 995340 . + 0 Rv0892 Rv0892 Function unknown; probably involved in cellular metabolism. Probable monooxygenase Rv0892, (MTCY31.20), len: 495 aa. Probable monooxygenase, highly similar to others e.g. NP_250787.1|NC_002516 probable flavin-binding monooxygenase from Pseudomonas aeruginosa (491 aa); CAB59668.1|AL132674 monooxygenase from Streptomyces coelicolor (519 aa); P12015|CYMO_ACIS cyclohexanone monooxygenase from Acinetobacter sp. (542 aa), FASTA scores: opt: 489, E(): 6.8e-26, (30.3% identity in 492 aa overlap); etc. Also highly similar to Rv0565c, Rv3854c, Rv3083, etc from Mycobacterium tuberculosis. Has hydrophobic stretch at N-terminus. P9WNG1 No intermediary metabolism and respiration P64745 P9WNG1 GO:0050661,GO:0004499,GO:0055114,GO:0050660 1.14.13.- P9WNG1 NC_000962.3 Mycobrowser_v5 CDS 995318 996295 . - 0 Rv0893c Rv0893c Possible methyltransferase Possible S-adenosylmethionine-dependent methyltransferase Rv0893c, (MTCY31.21c), len: 325 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), belongs in family with P96823|Rv0146|MTCI5.20 from Mycobacterium tuberculosis (310 aa), FASTA scores: opt: 784, E(): 0, (43.2% identity in 308 aa overlap); Rv0726c, Rv0731c, Rv3399, etc. Also shows some similarity with others e.g. SC9B5.10|T35930 hypothetical protein from Streptomyces coelicolor (303 aa); BSUB0008_141|Q45500 hypothetical 34.8 kDa protein from Bacillus subtilis (304 aa), FASTA scores: E(): 0.00033, (26.8% identity in 168 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WFI1 No lipid metabolism P64747 P9WFI1 GO:0008168 2.1.1.- P9WFI1 NC_000962.3 Mycobrowser_v5 CDS 1016236 1016685 . + 0 Rv0912 Rv0912 Unknown Probable conserved transmembrane protein Rv0912, (MTCY21C12.06), len: 149 aa. Probable conserved transmembrane protein, equivalent to Q50121|NP_302397.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (144 aa), FASTA scores: opt: 677, E(): 6.9e-38, (69.5% identity in 141 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O05904 No cell wall and cell processes O05904 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1024211 1024687 . + 0 Rv0918 Rv0918 Function unknown Conserved protein Rv0918, (MTCY21C12.12), len: 158 aa. Conserved protein, similar in part to Q50116 hypothetical protein from Mycobacterium leprae (44 aa), FASTA scores: opt: 132, E(): 0.0055, (65.6% identity in 32 aa overlap). Also some similarity in C-terminus with other hypothetical proteins e.g. NP_289961.1|NC_002655 hypothetical protein from Escherichia coli strain O157:H7 (94 aa); etc. O05910 No conserved hypotheticals O05910 O05910 NC_000962.3 Mycobrowser_v5 CDS 1158918 1159307 . - 0 Rv1034c Rv1034c Possibly required for the transposition of the insertion element IS1560. Probable transposase (fragment) Rv1034c, (MTCY10G2.15), len: 129 aa. Probable IS1560 transposase fragment, similar to part of Rv3387|E1202305|MTV004.45 (225 aa) (65.1% identity in 129 aa overlap). I6X043 No insertion seqs and phages I6X043 NC_000962.3 Mycobrowser_v5 CDS 997782 999299 . + 0 Rv0895 Rv0895 May be involved in synthesis of triacylglycerol Possible triacylglycerol synthase (diacylglycerol acyltransferase) Rv0895, (MTCY31.23), len: 505 aa. Possible triacylglycerol synthase (See Daniel et al., 2004); member of family with: Rv3740c, Rv3734c, Rv1425, Rv1760, etc. Shows some similarity with NP_301898.1|NC_002677 conserved membrane protein from Mycobacterium leprae (491 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WKA3 No lipid metabolism P67204 P9WKA3 GO:0006071,GO:0008610,GO:0004144 2.3.1.20 P9WKA3 NC_000962.3 Mycobrowser_v5 CDS 1032710 1033786 . - 0 Rv0926c Rv0926c Function unknown Conserved hypothetical protein Rv0926c, (MTCY21C12.20c), len: 358 aa. Conserved hypothetical protein, similar to Rv1059 conserved hypothetical protein from Mycobacterium tuberculosis (354 aa). Also shows some similarity to AF170923|AF170923_3 dihydrodipicolinate reductase from Mastigocladus laminosus (278 aa), FASTA scores: opt: 170, E(): 0.00088, (25.7% identity in 276 aa overlap). I6WZS8 No conserved hypotheticals O05918 I6WZS8 GO:0008839,GO:0009089,GO:0055114 NC_000962.3 Mycobrowser_v5 CDS 999472 1000767 . + 0 Rv0896 gltA2 Involved in tricarboxylic acid cycle (KREBS cycle) [catalytic activity: citrate + CoA = acetyl-CoA + H2O + oxaloacetate]. Probable citrate synthase I GltA2 Rv0896, (MTCY31.24), len: 431 aa. Probable gltA2, citrate synthase 1, highly similar to O33066|NP_302405.1|NC_002677 citrate synthase 1 from Mycobacterium leprae (431 aa), FASTA scores: E(): 0, (91.0 identity in 431 aa overlap); and AAF04133.1|AF191033_1|AF191033 citrate synthase from Mycobacterium smegmatis (441 aa). Also highly similar to others e.g. AAF14286.1|AF181118_1|AF181118 citrate synthase from Streptomyces coelicolor (429 aa); P42457|CISY_CORGL citrate synthase from Corynebacterium glutamicum (437 aa), FASTA scores: opt: 1847, E(): 0, (63.0% identity in 433 aa overlap); etc. Also similar to two other Mycobacterium tuberculosis citrate synthases, Rv0889|MTCY31.17c|citA (373 aa), FASTA score: (29.2% identity in 274 aa overlap) and Rv1131|MTCY22G8.20|gltA1 (393 aa). Contains PS00480 Citrate synthase signature. Belongs to the citrate synthase family. P9WPD5 No intermediary metabolism and respiration Q10530 P9WPD5 GO:0004108,GO:0005737,GO:0006099,GO:0044262 2.3.3.1 P9WPD5 NC_000962.3 Mycobrowser_v5 CDS 1000808 1002415 . - 0 Rv0897c Rv0897c Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv0897c, (MTCY31.25c), len: 535 aa. Possible oxidoreductase, similar to various oxidoreductases from diverse organisms e.g. CAB94055.1|AL358672 putative oxidoreductase from Streptomyces coelicolor (540 aa); NP_147877.1|NC_000854 phytoene dehydrogenase from Aeropyrum pernix (549 aa); Q01671|CRTD_RHOSH methoxyneurosporene dehydrogenase from Rhodobacter sphaeroides (495 aa), FASTA scores: opt: 139, E(): 2.6e-06, (23.8% identity in 538 aa overlap); etc. Also similar to Rv1432, Rv2997, and Rv3829c from Mycobacterium tuberculosis. P9WKP7 No intermediary metabolism and respiration P9WKP7 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1027685 1029337 . + 0 Rv0922 Rv0922 Required for the transposition of the insertion element IS1535 Possible transposase Rv0922, (MTCY21C12.16), len: 550 aa. Possible transposase for IS1535, similar to many e.g. YX16_MYCTU|Q10809|MTCY274.16c from Mycobacterium tuberculosis (460 aa), FASTA scores: opt 939, E(): 0, (40.6% identity in 465 aa overlap); etc. I6Y560 No insertion seqs and phages O05914 I6Y560 I6Y560 NC_000962.3 Mycobrowser_v5 CDS 1076196 1076678 . - 0 Rv0964c Rv0964c Unknown Hypothetical protein Rv0964c, (MTCY10D7.10), len: 160 aa. Hypothetical unknown protein. Equivalent to AAK45241.1 from Mycobacterium tuberculosis strain CDC1551 (138 aa) but longer 22 aa. P9WKM5 No conserved hypotheticals P9WKM5 NC_000962.3 Mycobrowser_v5 CDS 1142671 1143648 . + 0 Rv1021 Rv1021 Function unknown Conserved protein Rv1021, (MTCY10G2.28c), len: 325 aa. Conserved protein, similar to YBL1_STRCI|P33653 hypothetical 26.1 kDa protein from Streptomyces cacaoi (242 aa), FASTA scores: opt: 493, E(): 1.1e-23, (42.9% identity in 238 aa overlap). P96379 No conserved hypotheticals P96379 P96379 P96379 NC_000962.3 Mycobrowser_v5 CDS 1005852 1006562 . - 0 Rv0903c prrA Transcriptional regulator part of the two component regulatory system PRRA/PRRB. Thought to be involved in the environmental adaptation, specifically in an early phase of the intracellular growth. Two component response transcriptional regulatory protein PrrA Rv0903c, (MTCY31.31c), len: 236 aa. PrrA, two-component response regulator (see citations below), equivalent to Z99494|MLCB57_27|NP_302402.1|NC_002677 two-component response regulator from Mycobacterium leprae (233 aa), FASTA scores: opt: 1414, E(): 0, (95.7% identity in 233 aa overlap); and similar to T45446 probable two-component response regulator from Mycobacterium leprae (253 aa). Also similar to many sensor-like histidine kinase proteins e.g. CAB88489.1|AL353816 putative two-component systen response regulator from Streptomyces coelicolor (248 aa); AAG36759.1|AF119221_1 |AF119221 response regulator from Corynebacterium glutamicum (232 aa); Q02540|COPR_PSESM transcriptional activator protein COPR from Pseudomonas syringae (pv. tomato) (227 aa), FASTA scores: opt: 600, E(): 0, (44.4% identity in 225 aa overlap); etc. Also similar to Rv0981 from Mycobacterium tuberculosis (230 aa), Rv3765c (234 aa), phoP (247 aa), etc. Thought to be induced at phagocytosis (see Graham & Clark-Curtiss 1999). P9WGM1 No regulatory proteins 1YS6,1YS7 P0A5Z6 P9WGM1 GO:0005737,GO:0006355,GO:0006350,GO:0000156,GO:0000160,GO:0003677 P9WGM1 NC_000962.3 Mycobrowser_v5 CDS 1077975 1078334 . + 0 Rv0967 csoR Involved in transcriptional mechanism; repression of the CSO operon. Copper-sensitive operon repressor CsoR Rv0967, (MTCY10D7.07c), len: 119 aa. CsoR, copper-sensitive operon repressor, part of cso operon (See Liu et al., 2007), similar to hypothetical proteins from several organisms e.g. AE002074|AE002074_11 from Deinococcus radiodurans (102 aa), FASTA scores: opt: 233, E(): 8.6e-10, (47.0% identity in 83 aa overlap); O32222|Z99121|YVGZ from Bacillus subtilis (101 aa), FASTA scores: opt:228, E(): 3.2e-15, (38.0% identity in 92 aa overlap); etc. Also similar to Mycobacterium tuberculosis hypothetical proteins Rv0190, and Rv1766. P9WP49 No regulatory proteins 2HH7 P71543 P9WP49 GO:0005737,GO:0046872,GO:0045449,GO:0006350,GO:0003677 P9WP49 NC_000962.3 Mycobrowser_v5 CDS 1010136 1011734 . + 0 Rv0907 Rv0907 Function unknown, possibly involved in cell wall biosynthesis. Conserved protein Rv0907, (MTCY21C12.01), len: 532 aa. Conserved protein, possibly involved in cell wall biosynthesis: similar to many beta-lactamases, penicillin-binding proteins and hypothetical proteins e.g. NP_298910.1|NC_002488 beta-lactamase from Xylella fastidiosa (455 aa); Q06317|PBP4_NOCLA penicillin-binding protein 4 (PBP-4) (381 aa), FASTA scores: opt: 299, E(): 8.8e-05, (28.7% identity in 401 aa overlap); etc. N-terminus highly similar to AAA63047.1|U15184 hypothetical protein from Mycobacterium leprae (58 aa). Related to other putative esterases and penicillin binding proteins in Mycobacterium tuberculosis e.g. Rv1730c|MTCY04C12.15c (517 aa). O05900 No cell wall and cell processes O05900 O05900 O05900 NC_000962.3 Mycobrowser_v5 CDS 1017217 1018725 . - 0 Rv0913c Rv0913c Function unknown; probably involved in cellular metabolism. Possible dioxygenase Rv0913c, (MTCY21C12.07c), len: 502 aa. Possible dioxygenase, showing similarity with others e.g. AAK38744.1|AY029525 carotenoid 9,10-9',10' cleavage dioxygenase from Phaseolus vulgaris (543 aa); CAB56138.1|AL117669 putative dioxygenase from Streptomyces coelicolor (503 aa); AAK06796.1|AF324838_15|AF324838 putative dioxygenase SimC5 from Streptomyces antibioticus (456 aa); Q53353|S65040 lignostilbene-alpha,beta-dioxygenase from Pseudomonas paucimobilis (485 aa), FASTA scores: opt: 310, E(): 3.4e-20, (28.9% identity in 495 aa overlap); etc. Also some similarity with Rv0654|MTCI376.22 probable dioxygenase from Mycobacterium tuberculosis (501 aa). I6Y551 No intermediary metabolism and respiration O05905 I6Y551 GO:0016702,GO:0055114 1.-.-.- I6Y551 NC_000962.3 Mycobrowser_v5 CDS 1008207 1008938 . + 0 Rv0905 echA6 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Possible enoyl-CoA hydratase EchA6 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv0905, (MTCY31.33), len: 243 aa. Possible echA6, enoyl-CoA hydratase, highly similar to ML15184|U15184 enoyl-CoA hydratase from Mycobacterium leprae (247 aa), FASTA score: (85.8% identity in 247 aa overlap). Also similar to many e.g. NP_250320.1|NC_002516 probable enoyl-CoA hydratase/isomerase from Pseudomonas aeruginosa (261 aa); NP_415911.1|NC_000913 putative enzyme from Escherichia coli strain K12 (255 aa); P24162|ECHH_RHOCA|FADB1 enoyl-CoA hydratase homolog from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (257 aa), FASTA scores: opt: 404, E():7.8e-21, (37.3% identity in 249 aa overlap); etc. P9WNP1 No lipid metabolism 3HE2 P64014 P9WNP1 GO:0006631,GO:0004300 4.2.1.17 P9WNP1 NC_000962.3 Mycobrowser_v5 CDS 1014681 1014860 . + 0 Rv0909 Rv0909 Function unknown Conserved hypothetical protein Rv0909, (MTCY21C12.03), len: 59 aa. Conserved hypothetical protein, equivalent to NP_302399.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (56 aa). Also some similarity with AL022268|SC4H2_10c hypothetical protein from Streptomyces coelicolor (97 aa), FASTA scores: opt: 106, E(): 0.13, (43.2% identity in 37 aa overlap). P9WJ07 No conserved hypotheticals P9WJ07 NC_000962.3 Mycobrowser_v5 CDS 1014866 1015300 . + 0 Rv0910 Rv0910 Function unknown Conserved hypothetical protein Rv0910, (MTCY21C12.04), len: 144 aa. Conserved hypothetical protein, equivalent to NP_302398.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (181 aa), FASTA scores: opt: 820, E(): 0, (83.9% identity in 143 aa overlap). Also similar to Rv1546|MTCY48.19c hypothetical protein from Mycobacterium tuberculosis (143 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WJ05 No conserved hypotheticals O05902 P9WJ05 P9WJ05 NC_000962.3 Mycobrowser_v5 CDS 1015398 1016171 . + 0 Rv0911 Rv0911 Function unknown Conserved protein Rv0911, (MTCY21C12.05), len: 257 aa. Conserved protein, showing similarity with hydroxylases and hypothetical proteins e.g. T35325 probable hydroxylase from Streptomyces coelicolor (265 aa); Q54242 hypothetical protein from Streptomyces, FASTA scores: opt: 372, E(): 8.8e-18, (32.0% identity in 256 aa overlap); AAD04716.1|U77891 doxorubicin biosynthesis enzyme DnrV from Streptomyces peucetius (275 aa); AAA63051.1|U15184 hypothetical protein from Mycobacterium leprae (94 aa); etc. Also similar to Rv0577 hypothetical protein from Mycobacterium tuberculosis (261 aa). I6XA34 No conserved hypotheticals O05903 I6XA34 I6XA34 NC_000962.3 Mycobrowser_v5 CDS 1020058 1021329 . - 0 Rv0915c PPE14 Function unknown. Possibly a protective antigen involved with the early control of infection. PPE family protein PPE14 Rv0915c, (MTCY21C12.09c), len: 423 aa. PPE14 (alternate gene name: MTB41). Member of the Mycobacterium tuberculosis PPE family (see citation below), highly similar to many e.g. Rv1807 from Mycobacterium tuberculosis (403 aa), FASTA scores: opt: 966, E(): 4.4e-30, (45.7% identity in 392 aa overlap); etc. Contains PS00626 Regulator of chromosome condensation (RCC1) signature 2. P9WI33 No PE/PPE Q79FV1 P9WI33 P9WI33 NC_000962.3 Mycobrowser_v5 CDS 1021344 1021643 . - 0 Rv0916c PE7 Function unknown PE family protein PE7 Rv0916c, (MTCY21C12.10c), len: 99 aa. PE7 (alternate gene name: MTB10). Member of the Mycobacterium tuberculosis PE family (see citations below), similar to many e.g. Rv1788 from Mycobacterium tuberculosis (99 aa), FASTA scores: opt: 321, E(): 1.3e-11, (53.5% identity in 99 aa overlap); etc. I6Y936 No PE/PPE Q7D937 I6Y936 I6Y936 NC_000962.3 Mycobrowser_v5 CDS 1064114 1064962 . - 0 Rv0953c Rv0953c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0953c, (MTCY10D7.21), len: 282 aa. Possible oxidoreductase, equivalent to CAA48222.1|X68102 hypothetical protein from Mycobacterium avium subsp. paratuberculosis (166 aa). Similar to several hypothetical proteins and oxidoreductases e.g. AAK38097.1|AF323606_3|AF323606 putative F420-dependent dehydrogenase from Rhodococcus erythropolis (295 aa); NP_070025.1|NC_000917 N5,N10-methylenetetrahydromethanopterin reductase (mer-2) from Archaeoglobus fulgidus (348 aa); etc. Also similar to several hypothetical proteins and oxidoreductases from Mycobacterium tuberculosis e.g. Rv2161c|O06216|Z95388|MTCY270.07 (288 aa), FASTA scores: opt: 633, E(): 0, (40.4% identity in 277 aa overlap), Rv3079c (275 aa), Rv0791c (347 aa), etc. Contains PS00201 Flavodoxin signature. P9WKN5 No intermediary metabolism and respiration P64769 P9WKN5 P9WKN5 NC_000962.3 Mycobrowser_v5 CDS 1077233 1077835 . - 0 Rv0966c Rv0966c Function unknown Conserved protein Rv0966c, (MTCY10D7.08), len: 200 aa. Conserved protein, equivalent to AL035500|MLCL373_12 conserved hypothetical protein from Mycobacterium leprae (200 aa), FASTA scores: opt: 1080, E(): 0, (79.5% identity in 200 aa overlap). Also highly similar to SCE6.30c|CAB88834.1|AL353832 hypothetical protein from Streptomyces coelicolor (277 aa). Some similarity to Rv2862c|MTV007.08 conserved hypothetical protein from Mycobacterium tuberculosis (194 aa), FASTA scores: E(): 3.1e-06, (31.5% identity in 184 aa overlap). Equivalent to AAK45243.1 from Mycobacterium tuberculosis strain CDC1551 (230 aa) but shorter 30 aa. Note that Rv0966c has been shortened since first entry. P9WKM1 No conserved hypotheticals P71544 P9WKM1 P9WKM1 NC_000962.3 Mycobrowser_v5 CDS 2204866 2205273 . - 0 Rv1962c vapC35 Unknown Possible toxin VapC35. Contains PIN domain. Rv1962c, (MTCY09F9.02), len: 135 aa. Possible vapC35, toxin, part of toxin-antitoxin (TA) operon with Rv1962A, contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. Rv3408|MTCY78.20c (133 aa) (36.2% identity in 138 aa overlap); and Rv3384c (130 aa) (43.1% identity in 130 aa overlap) P9WF67 No virulence, detoxification, adaptation P95252 P9WF67 P9WF67 NC_000962.3 Mycobrowser_v5 CDS 1031896 1032633 . - 0 Rv0925c Rv0925c Function unknown Conserved protein Rv0925c, (MTCY21C12.19c), len: 245 aa. Conserved protein, similar to AL132991|SCF55_19 hypothetical protein from Streptomyces coelicolor (197 aa), FASTA scores: opt: 459, E(): 1.2e-23, (39.3% identity in 201 aa overlap). I6Y946 No conserved hypotheticals O05917 I6Y946 I6Y946 NC_000962.3 Mycobrowser_v5 CDS 1033840 1034631 . - 0 Rv0927c Rv0927c Function unknown; possibly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv0927c, (MTCY21C12.21c), len: 263 aa. Probable short-chain dehydrogenase/reductase, similar to various dehydrogenases/reductases, notably 7-alpha-hydroxysteroid dehydrogenases and glucose 1-dehydrogenases e.g. P25529|HDHA_ECOLI 7-alpha-hydroxysteroid dehydrogenase from Escherichia coli (255 aa), FASTA scores: opt: 551, E(): 1e-26, (39.5% identity in 248 aa overlap); NP_252778.1|NC_002516 probable short-chain dehydrogenase from Pseudomonas aeruginosa (253 aa); AAC44307.1|U59433 3-ketoacyl-acyl carrier protein reductase from Bacillus subtilis (246 aa); etc. Also similar to other dehydrogenases from Mycobacterium tuberculosis e.g. MTCY09F9.36, E():1.4e-18; MTCY369.14, E():8e-17; MTCY02B10.14, E():2.5e-14; MTCY09F9.23c, E():1.5e-13; MTCY03C7.07, E():1.9e-13. Contains PS00061 Short-chain dehydrogenases/reductases family signature, and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the short-chain dehydrogenases/reductases (SDR) family. P9WGQ5 No intermediary metabolism and respiration O05919 P9WGQ5 GO:0055114,GO:0016491 1.-.-.- P9WGQ5 NC_000962.3 Mycobrowser_v5 CDS 1034903 1036015 . + 0 Rv0928 pstS3 Involved in active transport of inorganic phosphate across the membrane (import). This is one of the proteins required for binding-protein-mediated phosphate transport. Periplasmic phosphate-binding lipoprotein PstS3 (PBP-3) (PstS3) (PHOS1) Rv0928, (MTCY21C12.22), len: 370 aa. PstS3 (previously known as phoS2), phosphate-binding lipoprotein component of inorganic phosphate transport system (see citations below), highly similar to others from Mycobacterium leprae e.g. Q50099|PSTS3|PHOS1 phosphate-binding protein 3 precursor (328 aa), FASTA scores: opt: 1772, E(): 0, (79.6% identity in 328 aa overlap); and highly similar to others e.g. AAF74819.1|AF137360_1|AF137360 periplasmic phosphate permease from Mycobacterium avium (369 aa). Also highly similar to Rv0932c|MTCY08D9.07|pstS2 phosphate-binding periplasmic lipoprotein (370 aa); and Rv0934|pstS1 phosphate-binding periplasmic lipoprotein (374 aa) from Mycobacterium tuberculosis (Mycobacterium tuberculosis seems to have three PstS-like proteins, others being Rv0932c and Rv0934c). Contains lipoprotein signature (PS00013) at N-terminus. Belongs to family of phosphate receptors for bacterial ABC-type lipoprotein transporters. P9WGT7 No cell wall and cell processes P0A5Y2 P9WGT7 GO:0006817,GO:0005886,GO:0006950,GO:0005315 P9WGT7 NC_000962.3 Mycobrowser_v5 CDS 1053765 1054241 . + 0 Rv0944 Rv0944 This enzyme may play a significant role in processes leading to recovery from mutagenesis and/or cell death by alkylating agents [catalytic activity: hydrolysis of DNA containing ring-opened N7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimide]. Possible formamidopyrimidine-DNA glycosylase (FAPY-DNA glycosylase) Rv0944, (MTCY10D7.30c), len: 158 aa. Possible formamidopyrimidine-DNA glycosylase, similar to C-terminus of formamidopyrimidine-DNA glycosylases e.g. CAB63194.1|AL133469 putative formamidopyrimidine-DNA glycosylase from Streptomyces coelicolor (287 aa); FPG_LACLA|NP_266509.1|NC_002662 formamidopyrimidine-DNA glycosylase from Lactococcus lactis subsp. lactis (273 aa), FASTA scores: opt: 246, E(): 2.4e-09, (28.9% identity in 142 aa overlap); O50606|FPG_THETH|MUTM|FPG formamidopyrimidine-DNA glycosylase from Thermus thermophilus (267 aa); etc. Also similar to C-termini of endonucleases or DNA glycosylases of Mycobacterium tuberculosis e.g. Rv3297, Rv2464c, Rv2924c. May belong to the FPG family. L0T864 No information pathways P71565 L0T864 GO:0006289,GO:0008534,GO:0008270,GO:0003684 3.2.2.23 L0T864 NC_000962.3 Mycobrowser_v5 CDS 1036999 1037925 . + 0 Rv0930 pstA1 Involved in active transport of inorganic phosphate across the membrane (import); responsible for the translocation of the substrate across the membrane. This is one of the proteins required for binding-protein-mediated phosphate transport. Probable phosphate-transport integral membrane ABC transporter PstA1 Rv0930, (MTCY21C12.24), len: 308 aa. Probable pstA1, phosphate-transport integral membrane ABC transporter (see citation below), highly similar to others e.g. NP_302393.1|NC_002677 membrane-bound component of phosphate transport from Mycobacterium leprae (304 aa); CAB88473.1|AL353816 phosphate ABC transport system permease protein from Streptomyces coelicolor (354 aa) (N-terminus longer); NP_312689.1|NC_002695 phosphate transport system permease protein PstA from Escherichia coli strain O157:H7 (296 aa); etc. Also similar to Rv0936|MTCY08D9.03c|PSTA2 probable transmembrane ABC transporter component of phosphate uptake system from Mycobacterium tuberculosis (301 aa). P9WG11 No cell wall and cell processes O86345 P9WG11 GO:0016021,GO:0006817,GO:0005886,GO:0005315 P9WG11 NC_000962.3 Mycobrowser_v5 CDS 1037920 1039914 . - 0 Rv0931c pknD Involved in signal transduction (via phosphorylation). Thought to regulate phosphate transport. Can phosphorylate the peptide substrate myelin basic protein (MBP) at serine and threonine residues. Can be autophosphorylated on threonine residues [catalytic activity: ATP + a protein = ADP + a phosphoprotein]. Transmembrane serine/threonine-protein kinase D PknD (protein kinase D) (STPK D) Rv0931c, (MTCY08D9.08), len: 664 aa. PknD (alternate gene name: mbk), transmembrane serine/threonine protein kinase (see citations below), equivalent to CAB62227.1|AJ250200 putative serine/threonine protein kinase from Mycobacterium bovis BCG (291 aa); and highly similar in N-terminus to P54744|PKNB_MYCLE probable serine/threonine-specific protein kinase from Mycobacterium leprae (622 aa). Also highly similar to others, particularly in N-terminal half e.g. NP_243370.1|NC_002570 serine/threonine protein kinase from Bacillus halodurans (664 aa); NP_268044.1|NC_002662 serine/threonine protein kinase from Lactococcus lactis (627 aa); etc. Also highly similar to other serine/threonine protein kinases from Mycobacterium tuberculosis e.g. pknH (626 aa), FASTA scores: opt: 1398, E: 0, (49.3% identity in 540 aa overlap); pknE (566 aa); pknB (626 aa); Rv3524 (343 aa); etc. Contains Hank's kinase subdomain. Contains two transmembrane segments, which flank a highly repetitive region, suggesting a receptor-like anchoring. Belongs to the Ser/Thr family of protein kinases. Experimental studies show evidence of auto-phosphorylation on a serine residue. Appears to be co-transcribed with Rv0932c|pstS2. P9WI79 No regulatory proteins 1RWI,1RWL O05871 P9WI79 GO:0006468,GO:0004674,GO:0005524 2.7.11.1 P9WI79 NC_000962.3 Mycobrowser_v5 CDS 1977328 1977885 . - 0 Rv1749c Rv1749c Unknown Possible integral membrane protein Rv1749c, (MTCY28.12c-MTCY04C12.33c), len: 185 aa. Possible integral membrane protein, similar to O27914|AE000940 hypothetical protein MTH1892 from Methanobacterium thermoautotrophicum (168 aa), fasta scores: E(): 9.3e-16, (37.4% identity in 123 aa overlap). O65935 No cell wall and cell processes O65935 O65935 NC_000962.3 Mycobrowser_v5 CDS 1041264 1042094 . + 0 Rv0933 pstB Involved in active transport of inorganic phosphate across the membrane (import); responsible for energy coupling to the transport system. This is one of the proteins required for binding-protein-mediated phosphate transport. Have ATP-binding ability and ATPase activity. Phosphate-transport ATP-binding protein ABC transporter PstB Rv0933, (MTCY08D9.06c), len: 276 aa. PstB, phosphate-transport ATP-binding protein ABC transporter (see citations below), thermostable ATPase, highly similar to others e.g. NP_348334.1|NC_003030 ATPase component of ABC-type phosphate transport system from Clostridium acetobutylicum (249 aa); NP_212352.1|NC_001318 phosphate ABC transporter ATP-binding protein (pstB) from Borrelia burgdorferi (260 aa); NP_390375.1|NC_000964 phosphate ABC transporter (ATP-binding protein) from Bacillus subtilis (269 aa), FASTA scores: opt: 762, E(): 0, (47.7% identity in 243 aa overlap); etc. Also similar to other M. tuberculosis ABC transporters e.g. MTCY253.24, E(): 2.5e-15 and MTCY359.14c, E(): 3.4e-15. Contains PS00211 ABC transporters family signature, and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters). Magnesium or calcium seem to have no influence on the functionality of this enzyme. P9WQK9 No cell wall and cell processes P95302 P9WQK9 GO:0005315,GO:0015415,GO:0006817,GO:0005886,GO:0005524 3.6.3.27 P9WQK9 NC_000962.3 Mycobrowser_v5 CDS 1043299 1044315 . + 0 Rv0935 pstC1 Involved in active transport of inorganic phosphate across the membrane (import); responsible for the translocation of the substrate across the membrane. This is one of the proteins required for binding-protein-mediated phosphate transport. Phosphate-transport integral membrane ABC transporter PstC1 Rv0935, (MTCY08D9.04c), len: 338 aa. PstC1, phosphate-transport integral membrane ABC transporter (see citations below), highly similar to others e.g. NP_104768.1|NC_002678|pstC phosphate ABC transporter permease protein from Mesorhizobium loti (327 aa); NP_245372.1|NC_002663|PstC PstC protein from Pasteurella multocida (320 aa); P45191|PSTC_HAEIN phosphate transport system permease from Haemophilus influenza (315 aa), FASTA scores: opt: 667, E(): 0, (36.2% identity in 309 aa overlap); etc. Also similar to Rv0929|MTCY21C12.23|PSTC2 probable transmembrane ABC transporter component of phosphate uptake system from Mycobacterium tuberculosis (324 aa), FASTA scores: opt: 487, E(): 4.1e-21, (32.3% identity in 303 aa overlap); and shows slight similarity to MTCY08D9.03c|PSTA2|Rv0936 probable transmembrane ABC transporter component of phosphate uptake system from Mycobacterium tuberculosis (301 aa). Contains binding-protein-dependent transport systems inner membrane comp signature (PS00402). P9WG07 No cell wall and cell processes P0A628 P9WG07 GO:0016021,GO:0006817,GO:0005886,GO:0005315 P9WG07 NC_000962.3 Mycobrowser_v5 CDS 1044317 1045222 . + 0 Rv0936 pstA2 Involved in active transport of inorganic phosphate across the membrane (import); responsible for the translocation of the substrate across the membrane. This is one of the proteins required for binding-protein-mediated phosphate transport. Phosphate-transport integral membrane ABC transporter PstA2 Rv0936, (MTCY08D9.03c), len: 301 aa. PstA2, phosphate-transport integral membrane ABC transporter (see citations below), highly similar to others e.g. NP_442269.1|NC_000911|PstA phosphate transport system permease protein from Synechocystis sp. strain PCC 6803 (287 aa); NP_232473.1|NC_002506 phosphate ABC transporter permease protein from Vibrio cholerae (289 aa); P07654|PSTA_ECOLI phosphate transport system permease from Escherichia coli (296 aa), FASTA scores: opt: 464, E(): 6.7e-24, (30.5% identity in 282 aa overlap); etc. Also similar to O86345|MTCY21C12.24|PSTA1|Rv0930 probable transmembrane ABC transporter component of phosphate uptake system from Mycobacterium tuberculosis (304 aa), FASTA scores: opt: 369, E(): 6.1e-15, (32.7% identity in 248 aa overlap). Contains binding-protein-dependent transport systems inner membrane comp signature (PS00402). P9WG09 No cell wall and cell processes P0A626 P9WG09 GO:0016021,GO:0006817,GO:0005886,GO:0005315 P9WG09 NC_000962.3 Mycobrowser_v5 CDS 1045199 1046020 . - 0 Rv0937c mku Involved in DNA double-strand break repair, by nonhomologous end joining (NHEJ). Interacts with LIGD (Rv0938). DNA end-binding protein, Mku Rv0937c, (MTCY08D9.02), len: 273 aa. Mku, DNA end-binding protein, highly similar to others e.g. SC6G9.24c|T35620|AL079356 hypothetical protein from Streptomyces coelicolor (365 aa), FASTA scores: opt: 648, E(): 0, (36.5% identity in 274 aa overlap); Z99110|BSUB0007_223|NP_389224.1|NC_000964 hypothetical proteins from Bacillus subtilis (311 aa), FASTA scores: opt: 623, E(): 1.1e-31, (33.9% identity in 274 aa overlap); O28548|AE000984|AF1726|NP_070554.1|NC_000917 conserved hypothetical protein from Archaeoglobus fulgidus (286 aa), FASTA scores: opt: 583, E(): 0, (36.6% identity in 262 aa overlap); etc. P9WKD9 No information pathways O05866 P9WKD9 GO:0003677,GO:0006303,GO:0004003 P9WKD9 NC_000962.3 Mycobrowser_v5 CDS 1050593 1051459 . - 0 Rv0940c Rv0940c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0940c, (MTCY10D7.34), len: 288 aa. Possible oxidoreductase, similar to hypothetical proteins and oxidoreductases e.g. AAK38097.1|AF323606_3|AF323606 putative F420-dependent dehydrogenase from Rhodococcus erythropolis (295 aa); AAG52987.1|AF040570|Rif17 putative alkanal monooxygenase from Amycolatopsis mediterranei (356 aa); etc. Also similar to putative oxidoreductases from Mycobacterium tuberculosis such as Rv0953c|P71557|YT21_MYCTU (282 aa), FASTA scores: opt: 311, E(): 3.7e-08, (31.0% identity in 248 aa overlap), Rv3079c (275 aa), Rv0791c (347 aa), etc. P9WKP1 No intermediary metabolism and respiration P64761 P9WKP1 P9WKP1 NC_000962.3 Mycobrowser_v5 CDS 2205277 2205549 . - 0 Rv1962A vapB35 Unknown Possible antitoxin VapB35 Rv1962A, len: 90 aa. Possible vapB35, antitoxin, part of toxin-antitoxin (TA) operon with Rv1962c, see Arcus et al. 2005. Similar to others in M. tuberculosis e.g. Rv3385c, Rv3407, Rv0626 P9WF17 No virulence, detoxification, adaptation P9WF17 P9WF17 NC_000962.3 Mycobrowser_v5 CDS 1048412 1050346 . + 0 Rv0939 Rv0939 Function unknown; probably involved in cellular metabolism, possibly in a degradation pathway. Possible bifunctional enzyme: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (HHDD isomerase) + cyclase/dehydrase Rv0939, (MTCY10D7.35c), len: 644 aa. Possible bifunctional enzyme, including 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase activity, and cyclase/dehydrase activity. N-terminal part similar to many isomerases e.g. NP_343861.1|NC_002754 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (hpcE-1) from Sulfolobus solfataricus (318 aa); NP_068932.1|NC_000917 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (hpcE-1) from Archaeoglobus fulgidus (324 aa), FASTA scores: opt: 400, E(): 5.8e-15, (33.9% identity in 289 aa overlap); etc. And C-terminal part highly similar to many cyclases/dehydrases e.g. AAK61721.1|AY033994 cyclase-like protein from Streptomyces aureofaciens (305 aa); CAC44204.1|AL593842 cyclase from Streptomyces coelicolor (297 aa), FASTA scores: opt: 375, E(): 2.7e-26, (35.6% identity in 284 aa overlap); NP_343860.1|NC_002754 putative Cyclase/dehydrase from Sulfolobus solfataricus (308 aa); etc. Also similar to Rv2993c hypothetical protein from Mycobacterium tuberculosis. O86346 No intermediary metabolism and respiration O86346 O86346 GO:0016853,GO:0008152,GO:0016787 5.3.3.- O86346 NC_000962.3 Mycobrowser_v5 CDS 1052696 1053736 . - 0 Rv0943c Rv0943c Function unknown; probably involved in cellular metabolism. Probable monooxygenase Rv0943c, (MTCY10D7.31), len: 346 aa. Possible monooxygenase, similar in part to others e.g. NP_250229.1|NC_002516 probable flavin-containing monooxygenase from Pseudomonas aeruginosa (527 aa); AAC36351.1|AF090329 cyclohexanone monooxygenase homolog from Pseudomonas fluorescens (437 aa); CAB59668.1|AL132674 monooxygenase from Streptomyces coelicolor (519 aa); etc. Also similar to putative monooxygenases from Mycobacterium tuberculosis e.g. Rv1393c|P71662|CY21B4.10C (492 aa). FASTA scores: opt: 129, E(): 8.5e-21, (27.5% identity in 236 aa overlap); Rv0892 (495 aa); Rv3049c (524 aa); etc. P9WKN7 No intermediary metabolism and respiration P9WKN7 P9WKN7 NC_000962.3 Mycobrowser_v5 CDS 1112384 1113460 . + 0 Rv0996 Rv0996 Unknown Probable conserved transmembrane protein Rv0996, (MTCI237.10), len: 358 aa. Probable conserved transmembrane protein, equivalent to AL035500|MLCL373_25 putative membrane protein from Mycobacterium leprae (342 aa), FASTA scores: (66.4% identity in 360 aa overlap). Contains possible signal sequence and other hydrophobic domains. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O05579 No cell wall and cell processes O05579 GO:0016021 O05579 NC_000962.3 Mycobrowser_v5 CDS 1057300 1057530 . - 0 Rv0947c Rv0947c Function unknown; thought to be involved in mycolic acid biosynthesis. Probable mycolyl transferase, pseudogene Rv0947c, (MTCY10D7.27), len: 76 aa. Probable mycolyl transferase pseudogene, similar to part of P31953|A85C_MYCTU|fbpC2 antigen 85-c precursor (85c) (fibronectin-binding protein C) from Mycobacterium tuberculosis (340 aa), FASTA scores: opt: 213, E(): 2e-08, (69.6% identity in 46 aa overlap). Yes lipid metabolism NC_000962.3 Mycobrowser_v5 CDS 1057646 1057963 . - 0 Rv0948c Rv0948c Involved in the shikimate pathway. Converts chorismate to prephenate in the biosynthesis of tyrosine and phenylalanine. Chorismate mutase Rv0948c, (MTCY10D7.26), len: 105 aa. Chorismate mutase, AroQ class (See Prakash et al., 2005; Schneider et al., 2008), equivalent to NP_301237.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (105 aa). Also similar (except in N-terminus) to SCD63.16c|CAB82023.1|AL161755 hypothetical protein from Streptomyces coelicolor (110 aa); and to N-terminus of two chorismate mutase/prephenate dehydratase. P9WIC1 No intermediary metabolism and respiration 2QBV,2VKL,2W19,2W1A P64767 P9WIC1 GO:0046417 P9WIC1 NC_000962.3 Mycobrowser_v5 CDS 1060656 1061654 . - 0 Rv0950c Rv0950c Function unknown Conserved hypothetical protein Rv0950c, (MTCY10D7.24), len: 332 aa. Conserved hypothetical protein, highly similar to AL035500|MLCL373.02c|T45433 hypothetical protein from Mycobacterium leprae (343 aa), FASTA scores: opt: 1500, E(): 0, (71.0% identity in 331 aa overlap). C-terminus highly similar to part of various proteins e.g. C-terminal part of NP_441943.1|NC_000911|NlpD lipoprotein from Synechocystis sp (715 aa); N-terminal part of NP_066789.1|NC_002576 putative peptidase from Rhodococcus equi (546 aa); C-terminal part of NP_212396.1|NC_001318 conserved hypothetical protein from Borrelia burgdorferi (417 aa); C-terminal part of P33648|NLPD_ECOLI|nlpd lipoprotein from Escherichia coli (379 aa), FASTA scores: opt: 276, E(): 2e-10, (29.9% identity in 234 aa overlap); etc. P71560 No conserved hypotheticals P71560 P71560 GO:0006508,GO:0004222 P71560 NC_000962.3 Mycobrowser_v5 CDS 1075297 1076097 . - 0 Rv0963c Rv0963c Function unknown Conserved hypothetical protein Rv0963c, (MTCCY10D7.11), len: 266 aa. Conserved hypothetical protein, similar in part to other conserved hypothetical proteins from Mycobacterium tuberculosis e.g. Rv2797c|MTCY16B7.46 (562 aa), FASTA scores: E(): 1.2e-23, (39.0% identity in 254 aa overlap); Rv2542 (403 aa); Rv2079 (656 aa). Also similar in part to AL133423|SC4A7_3 hypothetical secreted protein from Streptomyces coelicolor (406 aa), FASTA scores: opt: 231, E(): 6.8e-07, (31.4% identity in 204 aa overlap); and SCH10.21c|T36533 hypothetical protein from Streptomyces coelicolor (329 aa). P9WKM7 No conserved hypotheticals P64777 P9WKM7 P9WKM7 NC_000962.3 Mycobrowser_v5 CDS 3136330 3136599 . - 0 Rv2828A Rv2828A Unknown Conserved hypothetical protein Rv2828A, len: 89 aa. Conserved hypothetical protein, present in many mycobacteria. Equivalent to BCG2848c and Mb2852A (100% identity to both in 89 aa overlap) I6YAC9 No conserved hypotheticals I6YAC9 NC_000962.3 Mycobrowser_v5 CDS 1063140 1064051 . + 0 Rv0952 sucD Involved in tricarboxylic acid cycle [catalytic activity: ATP + succinate + CoA = ADP + succinyl-CoA + phosphate]. Probable succinyl-CoA synthetase (alpha chain) SucD (SCS-alpha) Rv0952, (MTCY10D7.22c), len: 303 aa. Probable sucD, succinyl-CoA synthetase, alpha chain, equivalent to AL035500|MLCL373_4|NP_301242.1|NC_002677 succinyl-CoA synthase [alpha] chain from Mycobacterium leprae (300 aa), FASTA score: (86.3% identity in 300 aa overlap). Also highly similar to others e.g. CAB92672.1|AL356832 from Streptomyces coelicolor (294 aa); P53591|SUCD_COXBU from Escherichia coli (288 aa), FASTA scores: opt: 855, E(): 0, (53.8% identity in 286 aa overlap); etc. Contains PS00399 ATP-citrate lyase and succinyl-CoA ligases active site, and PS00017 ATP/GTP-binding site motif A (P-loop). P9WGC7 No intermediary metabolism and respiration P71558 P9WGC7 GO:0003878,GO:0004775,GO:0006099,GO:0005524 6.2.1.5 P9WGC7 NC_000962.3 Mycobrowser_v5 CDS 1065127 1066038 . + 0 Rv0954 Rv0954 Unknown Probable conserved transmembrane protein Rv0954, (MTCY10D7.20c), len: 303 aa. Probable conserved transmembrane protein, highly similar to 34KD_MYCPA|Q04959 34 kDa antigenic protein from Mycobacterium paratuberculosis (298 aa), FASTA scores: opt: 1023, E(): 7.2e-36, (59.3% identity in 305 aa overlap); AAC69251.1|U82111 34 kDa antigen precursor from Mycobacterium leprae (336 aa); and AL035500|MLCL373.06 hypothetical membrane protein from Mycobacterium leprae (297 aa), FASTA score: (55.6% identity in 315 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WIR9 No cell wall and cell processes P9WIR9 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1066078 1067445 . + 0 Rv0955 Rv0955 Unknown Probable conserved integral membrane protein Rv0955, (MTCY10D7.19c), len: 455 aa. Probable conserved integral membrane protein, highly similar to AL035500|MLCL373_6 putative membrane protein from Mycobacterium leprae (430 aa), FASTA score: (75.9% identity in 419 aa overlap); and AAL05878.1|AF411607_2|AF411607 unknown protein from Mycobacterium avium subsp. paratuberculosis (409 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WKN3 No cell wall and cell processes P9WKN3 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1088493 1090175 . - 0 Rv0976c Rv0976c Function unknown Conserved hypothetical protein Rv0976c, (MTV044.04c), len: 560 aa. Conserved hypothetical protein, highly similar to others e.g. CAB95890.1|AL359988 conserved hypothetical protein from Streptomyces coelicolor (558 aa); P_251576.1|NC_002516 hypothetical protein from Pseudomonas aeruginosa (600 aa); etc. N-terminal part highly similar to AL035500|MLCL373_14 probable pseudogene from Mycobacterium leprae (163 aa), FASTA score: (50.0% identity in 122 aa overlap). O86320 No conserved hypotheticals O86320 O86320 O86320 NC_000962.3 Mycobrowser_v5 CDS 1069883 1071262 . + 0 Rv0958 Rv0958 Chelation, introducing a magnesium ion into specific substrate. Possible magnesium chelatase Rv0958, (MTCY10D7.16c), len: 459 aa. Possible magnesium chelatase, similar to others (especially in N-terminal parts) e.g. NP_296313.1|NC_001263|AE002088_10 putative magnesium protoporphyrin chelatase from Deinococcus radiodurans (487 aa), FASTA scores: opt: 1148, E(): 0, (42.4% identity in 450 aa overlap); Q44498|CHLI_ANAVA magnesium-chelatase subunit CHLI from Anabaena variabilis (338 aa); T31460 probable magnesium chelatase chain I bchI from Heliobacillus mobilis (363 aa); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). P71552 No intermediary metabolism and respiration P71552 P71552 GO:0005524,GO:0005622,GO:0016829,GO:0006355,GO:0008134 4.99.1.- P71552 NC_000962.3 Mycobrowser_v5 CDS 1071255 1073273 . + 0 Rv0959 Rv0959 Function unknown Conserved hypothetical protein Rv0959, (MTCY10D7.15c), len: 672 aa. Conserved hypothetical protein, similar to AE002069|AE002069_12 hypothetical protein from Deinococcus radiodurans (403 aa), FASTA scores: opt: 395, E(): 1.3e-15, (26.8% identity in 426 aa overlap). Contains a single copy at the N-terminus of a short repeat found three times in the M. tuberculosis ORF O33341|MTV003.05c|AL008883. P9WKN1 No conserved hypotheticals P9WKN1 NC_000962.3 Mycobrowser_v5 CDS 1073327 1073548 . + 0 Rv0959A vapB9 Unknown Possible antitoxin VapB9 Rv0959A, len: 73 aa. Possible vapB9, antitoxin, part of toxin-antitoxin (TA) operon with Rv0960 (See Arcus et al., 2005; Pandey and Gerdes, 2005). Weakly similar to others in Mycobacterium tuberculosis e.g. Rv1721c P9WJ55 No virulence, detoxification, adaptation P9WJ55 NC_000962.3 Mycobrowser_v5 CDS 1076778 1077197 . - 0 Rv0965c Rv0965c Function unknown Conserved hypothetical protein Rv0965c, (MTCY10D7.09), len: 139 aa. Conserved hypothetical protein, showing weak similarity with Rv2798c|MTCY16B7.45 conserved hypothetical protein from Mycobacterium tuberculosis (108 aa), FASTA scores: E(): 5.6e-12, (38.9% identity in 90 aa overlap). Equivalent to AAK45242.1 from Mycobacterium tuberculosis strain CDC1551 (146 aa) but shorter 7 aa. P9WKM3 No conserved hypotheticals P9WKM3 NC_000962.3 Mycobrowser_v5 CDS 1081052 1081684 . + 0 Rv0970 Rv0970 Unknown Probable conserved integral membrane protein Rv0970, (MTCY10D7.04c), len: 210 aa. Probable conserved integral membrane protein, part of cso operon, equivalent to NP_302348.1|NC_002677 probable integral membrane protein from Mycobacterium leprae (210 aa). P9WKL7 No cell wall and cell processes P9WKL7 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1081775 1082584 . - 0 Rv0971c echA7 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Probable enoyl-CoA hydratase EchA7 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv0971c, (MTCY10D7.03), len: 269 aa. Probable echA7, enoyl-CoA hydratase, similar to many e.g. CAB95895.1|AL359988 putative enoyl CoA hydratase from Streptomyces coelicolor (247 aa); P24162|ECHH_RHOCA enoyl-CoA hydratase from Rhodobacter capsulatus (257 aa), FASTA scores: opt: 369, E(): 2.6e-15, (33.7% identity in 246 aa overlap); etc. P71540 No lipid metabolism P71540 P71540 GO:0016853,GO:0008152,GO:0004300 4.2.1.17 P71540 NC_000962.3 Mycobrowser_v5 CDS 1082584 1083750 . - 0 Rv0972c fadE12 Function unknown, but involved in lipid degradation. Acyl-CoA dehydrogenase FadE12 Rv0972c, (MTCY10D7.02), len: 388 aa. fadE12, acyl-CoA dehydrogenase, highly similar to many e.g. CAB95893.1|AL359988 putative acyl CoA dehydrogenase from Streptomyces coelicolor (382 aa); P45857|ACDB_BACSU from Bacillus subtilis (379 aa), FASTA scores: opt: 576, E(): 2.3e-26, (29.7% identity in 381 aa overlap); etc. P9WQG3 No lipid metabolism P71539 P9WQG3 GO:0003995,GO:0055114,GO:0050660 1.3.99.- P9WQG3 NC_000962.3 Mycobrowser_v5 CDS 1085756 1087345 . - 0 Rv0974c accD2 Involved in fatty acid metabolism. Probable acetyl-/propionyl-CoA carboxylase (beta subunit) AccD2 Rv0974c, (MTV044.02c), len: 529 aa. Probable accD2, acetyl-/propionyl-CoA carboxylase (beta subunit), highly similar to many e.g. CAB95891.1|AL35998 putative acetyl/propionyl CoA carboxylase beta subunit from Streptomyces coelicolor (532 aa); NP_250704.1|NC_002516 probable acyl-CoA carboxyltransferase beta chain from Pseudomonas aeruginosa (535 aa); BAB16296.1|AB039884 acetyl-CoA carboxylase carboxyltransferase from Myxococcus xanthus (538 aa); NP_420973.1|NC_002696 putative propionyl-CoA carboxylase beta subunit from Caulobacter crescentus (530 aa); etc. Also similar to other from Mycobacterium tuberculosis: Rv2502c|ACCD1, Rv3799c|ACCD4, etc. Could belong to the ACCD/PCCB family. O86318 No lipid metabolism O86318 O86318 GO:0016874 6.4.1.- O86318 NC_000962.3 Mycobrowser_v5 CDS 1994671 1995054 . - 0 Rv1761c Rv1761c Unknown Possible exported protein Rv1761c, (MTCY28.27c), len: 127 aa. Possibly exported protein with hydrophobic stretch or TMhelix at aa 15-37. O06796 No cell wall and cell processes 2K3M O06796 O06796 NC_000962.3 Mycobrowser_v5 CDS 1094886 1095059 . + 0 Rv0979A rpmF Involved in translation mechanism. 50S ribosomal protein L32 RpmF Rv0979A, len: 57 aa. rpmF, 50S ribosomal protein L32, similar to others e.g. rpmF|Q9RL50 probable 50S ribosomal protein from Streptomyces coelicolor (56 aa), FASTA scores: E(): 5.1e-09, (63.45% identity in 52 aa overlap); etc. Belongs to the L32P family of ribosomal proteins. P0A5V8 No information pathways P0A5V8 P0A5V8 GO:0003735,GO:0006412,GO:0015934 NC_000962.3 Mycobrowser_v5 CDS 1093361 1094356 . - 0 Rv0978c PE_PGRS17 Function unknown PE-PGRS family protein PE_PGRS17 Rv0978c, (MTV044.06c), len: 331 aa. PE_PGRS17, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to others e.g. Z95387|MTCY1A10_19 from Mycobacterium tuberculosis (461 aa), FASTA score: (73.6% identity in 277 aa overlap); etc. Q79FU2 No PE/PPE Q79FU2 Q79FU2 Q79FU2 NC_000962.3 Mycobrowser_v5 CDS 1094670 1094864 . - 0 Rv0979c Rv0979c Unknown Hypothetical protein Rv0979c, (MTV044.07c), len: 64 aa (unlikely ORF). Hypothetical unknown protein. Start codon changed since first submission (-44 aa). O53892 No conserved hypotheticals O53892 NC_000962.3 Mycobrowser_v5 CDS 1162549 1163376 . - 0 Rv1040c PE8 Function unknown PE family protein PE8 Rv1040c, (MTCY10G2.09), len: 275 aa. PE8, Member of the Mycobacterium tuberculosis PE family (see citation below), most similar to AL008967|MTV002_34 Mycobacterium tuberculosis H37Rv (275 aa), FASTA scores: opt: 1111, E(): 0, (68.6% identity in 283 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L7N667 No PE/PPE Q7D8Y6 L7N667 L7N667 NC_000962.3 Mycobrowser_v5 CDS 1095078 1096451 . - 0 Rv0980c PE_PGRS18 Function unknown PE-PGRS family protein PE_PGRS18 Rv0980c, (MTV044.08c), len: 457 aa. PE_PGRS18, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002), highly similar to others e.g. Z95387|MTCY1A10_19 from Mycobacterium tuberculosis (461 aa), FASTA score: (66.7% identity in 405 aa overlap); Z95844|MTCY493_2 from Mycobacterium tuberculosis (741 aa), FASTA score: (53.0% identity in 394 aa overlap); etc. Predicted to be an outer membrane protein (See Song et al., 2008). Q79FU0 No PE/PPE Q79FU0 Q79FU0 Q79FU0 NC_000962.3 Mycobrowser_v5 CDS 1108578 1109171 . - 0 Rv0992c Rv0992c Function unknown Conserved hypothetical protein Rv0992c, (MTCI237.06c), len: 197 aa. Conserved hypothetical protein, equivalent to NP_301256.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (197 aa). Also similar, except in N-terminus, to other hypothetical proteins and ligases e.g. SCE87.34|CAB59679.1|AL132674 hypothetical protein from Streptomyces coelicolor (204 aa); NP_461977.1|NC_003197 putative ligase from Salmonella typhimurium (182 aa); P09160|YGFA_ECOLI hypothetical 21.1 kDa protein from Escherichia coli (182 aa), FASTA scores: opt: 191, E(): 1.1e-09, (29.5% identity in 146 aa overlap); etc. O05575 No conserved hypotheticals O05575 O05575 GO:0005524,GO:0009396,GO:0030272 O05575 NC_000962.3 Mycobrowser_v5 CDS 1114293 1114724 . + 0 Rv0997 Rv0997 Unknown Hypothetical protein Rv0997, (MTCI237.11), len: 143 aa. Hypothetical unknown protein, equivalent to AAK45276.1 from Mycobacterium tuberculosis strain CDC1551 (87 aa) but longer 56 aa. O05580 No conserved hypotheticals O05580 NC_000962.3 Mycobrowser_v5 CDS 1099066 1100460 . + 0 Rv0983 pepD Function unknown; possibly hydrolyzes peptides and/or proteins (seems to cleave preferentially after serine residues). Probable serine protease PepD (serine proteinase) (MTB32B) Rv0983, (MTV044.11), len: 464 aa. Probable pepD (alternate gene name: mtb32b), secreted or membrane serine protease (see citation below), equivalent (but longer 18 aa in N-terminus) to AL035500|MLCL373_17|T45448 probable serine proteinase from Mycobacterium leprae (452 aa), FASTA score: (74.2% identity in 466 aa overlap); and highly similar to others from Mycobacterium leprae. Also highly similar (except in N-terminus) to other proteases e.g. CAC01350.1|AL390975 putative protease from Streptomyces coelicolor (542 aa); NP_440705.1|NC_000911|HtrA serine protease from Synechocystis sp. (452 aa); NP_346646.1|NC_003028 serine protease from Streptococcus pneumoniae (393 aa); etc. Also similar in part to members of the htrA-antigen family e.g. U87242|MTU87242_3|HtrA serine protease from M. tuberculosis (542 aa), FASTA scores: opt: 846, E(): 2e-28, (40.6% identity in 392 aa overlap); and similar to other hypothetical serine proteases e.g. Rv0983, Rv0125, etc. Belongs to the serine protease family. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). O53896 No intermediary metabolism and respiration 1Y8T,2Z9I O53896 O53896 GO:0006508,GO:0004252,GO:0005515 3.4.21.- O53896 NC_000962.3 Mycobrowser_v5 CDS 1100460 1101005 . + 0 Rv0984 moaB2 Thought to be involved in molybdopterin biosynthesis. Catalyzes the dehydratation of 4A-hydroxytetrahydropterins [catalytic activity: (6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4A-hydroxypterin = (6R)-6-(L-erythro-1,2- dihydroxypropyl)-7,8-dihydro-6H-pterin + H(2)O.]. Possible pterin-4-alpha-carbinolamine dehydratase MoaB2 (PHS) (4-alpha- hydroxy-tetrahydropterin dehydratase) (pterin-4-a-carbinolamine dehydratase) (phenylalanine hydroxylase-stimulating protein) (PHS) (pterin carbinolamine dehydratase) (PCD) Rv0984, (MTV044.12), len: 181 aa. Possible moaB2, pterin-4-alpha-carbinolamine dehydratase, highly similar to NP_301253.1|NC_002677 putative molybdenum cofactor biosynthesis protein from Mycobacterium leprae (181 aa), FASTA score: (92.3% identity in 181 aa overlap). Also similar to others e.g. CAB59675.1|AL132674 molybdenum cofactor biosynthesis protein from Streptomyces coelicolor (179 aa); Q56208|MOCB_SYNP7 molybdenum cofactor biosynthesis protein CB from Synechococcus sp. (319 aa), FASTA score: (37.3% identity in 142 aa overlap); C-terminus of NP_197599.1|NC_003076 molybdopterin biosynthesis CNX1 protein from Arabidopsis thaliana (670 aa); etc. Also similar to Rv0865|MOG from Mycobacterium tuberculosis (160 aa); and other mog proteins e.g. CAC39235.1|AJ312124 Mog protein from Eubacterium acidaminophilum (162 aa). Could belong to the pterin-4-alpha-carbinolamine dehydratase family. Alternative start codon has been suggested in position 1100508. O53897 No intermediary metabolism and respiration O53897 O53897 GO:0016829,GO:0006777 O53897 NC_000962.3 Mycobrowser_v5 CDS 1122222 1123598 . - 0 Rv1005c pabB Catalyzes the biosynthesis of 4-amino-4-deoxychorismate (ADC) from chorismate and glutamine Probable para-aminobenzoate synthase component I PABD Rv1005c, (MTCI237.22c), len: 458 aa (Start-site not certain). Probable PabD, para-aminobenzoate synthase component I. Similar to PABB_ECOLI|P05041 para-aminobenzoate synthase component I from Escherichia coli (453 aa), FASTA scores: opt: 589, E(): 1.8e-27, (40.7% identity in 268 aa overlap). Similar to M. tuberculosis Rv1609, Rv3215, Rv2386c. O05591 No intermediary metabolism and respiration O05591 O05591 GO:0016829,GO:0009396 4.1.3.- O05591 NC_000962.3 Mycobrowser_v5 CDS 1102542 1105109 . + 0 Rv0987 Rv0987 Thought to be involved in active transport of adhesion component across the membrane: involved in atachment and virulence. Responsible for the translocation of the substrate across the membrane. Probable adhesion component transport transmembrane protein ABC transporter Rv0987, (MTV044.15, MTCI237.01), len: 855 aa. Probable transmembrane protein ABC transporter supposedly involved in transport of adhesion component (see citation below), whose N-terminus shows similarity with hypothetical proteins, generally transmembrane proteins, e.g. CAB96016.1|AL360055 putative ABC transport system integral membrane protein from Streptomyces coelicolor (855 aa); P44252|YCFU_HAEIN|HI1555 hypothetical protein from Haemophilus influenzae (393 aa), FASTA scores: opt: 265, E(): 1.7e-09, (23.6% identity in 402 aa overlap); etc. N- and C-termini respectively show similarity to O32735 ATTF protein (420 aa), FASTA scores: E(): 1e-09, (26.7% identity in 430 aa overlap), and G2340078 ATTG protein (359 aa), FASTA scores: E(): 2.7e-08, (27.8% identity in 356 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Believed to have been acquired by horizontal gene transfer (See Rosas-Magallanes et el., 2006; Becq et al., 2007). O53900 No cell wall and cell processes O53900 O53900 GO:0005886,GO:0016021 O53900 NC_000962.3 Mycobrowser_v5 CDS 1105116 1106276 . + 0 Rv0988 Rv0988 Unknown Possible conserved exported protein Rv0988, (MTCI237.02), len: 386 aa. Possible conserved exported protein, with potential N-terminal signal sequence, similar (except in N-terminus) to O32737|L63540 ATTH protein from Agrobacterium tumefaciens (355 aa), FASTA scores: opt: 651, E(): 5.7e-33, (33.4% identity in 344 aa overlap); and NP_231265.1|NC_002505 conserved hypothetical protein from Vibrio cholerae (372 aa). Predicted to be an outer membrane protein (See Song et al., 2008). Believed to have been acquired by horizontal gene transfer (See Rosas-Magallanes et el., 2006; Becq et al., 2007). O86370 No cell wall and cell processes O86370 O86370 GO:0015995,GO:0055114,GO:0016491,GO:0016117 O86370 NC_000962.3 Mycobrowser_v5 CDS 1144564 1145853 . + 0 Rv1023 eno Glycolysis [catalytic activity:2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O] Probable enolase Eno Rv1023, (MTCY10G2.26c), len: 429 aa. Probable eno, enolase, highly similar to others e.g. ENO_ECOLI|P08324 enolase from Escherichia coli (431 aa), FASTA scores: opt: 1487, E(): 0, (55.5% identity in 422 aa overlap); etc. Magnesium is required for catalysis and for stabilizing the dimer. Belongs to the enolase family. P9WNL1 No intermediary metabolism and respiration P96377 P9WNL1 GO:0005576,GO:0006096,GO:0000287,GO:0004634,GO:0000015,GO:0009986 4.2.1.11 P9WNL1 NC_000962.3 Mycobrowser_v5 CDS 2258030 2258272 . + 0 Rv2009 vapB15 Unknown Antitoxin VapB15 Rv2009, (MTCY39.08c), len: 80 aa. VapB15, antitoxin, part of toxin-antitoxin (TA) operon with Rv2010 (See Arcus et al., 2005; Pandey and Gerdes, 2005). P9WLM7 No virulence, detoxification, adaptation Q10848 P9WLM7 P9WLM7 NC_000962.3 Mycobrowser_v5 ncRNA 1175225 1175315 . + 0 MTB000061 mpr5 Unknown Fragment of putative small regulatory RNA mpr5, fragment of putative small regulatory RNA (See DiChiara et al., 2010), ends not mapped, ~100 nt band detected by Northern blot. No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 1107443 1108099 . - 0 Rv0990c Rv0990c Unknown Hypothetical protein Rv0990c, (MTCI237.04c), len: 218 aa. Hypothetical unknown protein. O05573 No conserved hypotheticals O05573 O05573 NC_000962.3 Mycobrowser_v5 CDS 1110269 1111549 . + 0 Rv0994 moeA1 Involved in molybdenum cofactor biosynthesis: involved in the biosynthesis of a demolybdo-cofactor (molybdopterin), necessary for molybdo-enzymes. Probable molybdopterin biosynthesis protein MoeA1 Rv0994, (MTCI237.08), len: 426 aa. Probable moeA1, molybdenum cofactor biosynthesis protein, equivalent to AL035500|MLCL373_23 putative molybdopterin biosynthesis protein from Mycobacterium leprae (424 aa), FASTA score: (88.3% identity in 426 aa overlap). Also highly similar to many e.g. CAB59677.1|AL132674 molybdopterin biosynthesis protein from Streptomyces coelicolor (424 aa); NP_385769.1|NC_003047 probable molybdopterin biosynthesis protein from Sinorhizobium meliloti (406 aa); P12281|MOEA_ECOLI molybdopterin biosynthesis moea protein from Escherichia coli (411 aa), FASTA scores: opt: 519, E(): 1.3e-24, (32.3% identity in 402 aa overlap); etc. Also similar to MOEA2|Rv0438c|MTV037.02c probable molybdopterin biosynthesis protein from Mycobacterium tuberculosis (405 aa). Note that previously known as moeA. P9WJQ7 No intermediary metabolism and respiration O05577 P9WJQ7 GO:0046872,GO:0016740,GO:0006777 2.-.-.- P9WJQ7 NC_000962.3 Mycobrowser_v5 CDS 1111612 1112223 . + 0 Rv0995 rimJ Acetylates the N-terminal alanine of ribosomal protein S5 [catalytic activity: acetyl-CoA + ribosomal-protein L-alanine = CoA + ribosomal-protein N-acetyl-L-alanine]. Ribosomal-protein-alanine acetyltransferase RimJ (acetylating enzyme for N-terminal of ribosomal protein S5) Rv0995, (MTCI237.09), len: 203 aa. RimJ, ribosomal-protein-alanine acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Equivalent to AL035500|MLCL373_24 probable acyltransferase from Mycobacterium leprae (218 aa), FASTA scores: (86.0% identity in 200 aa overlap). Also similar to others and many acyltransferases e.g. BAB69252.1|AB070946 possible acyltransferase from Streptomyces avermitilis (156 aa); NP_385025.1|NC_003047 probable ribosomal-protein-alanine acetyltransferase from Sinorhizobium meliloti (203 aa); P09454|RIMJ_ECOLI|B1066|Z1703|ECS1444 ribosomal-protein-alanine acetyltransferase from Escherichia coli strains K12 and O157:H7 (194 aa), FASTA scores: opt: 247, E(): 1.5e-10, (26.9% identity in 186 aa overlap). Belongs to the acetyltransferase family, RIMJ subfamily. O05578 No information pathways O05578 GO:0008999,GO:0005840,GO:0008152 2.3.1.128 O05578 NC_000962.3 Mycobrowser_v5 CDS 1160855 1161151 . - 0 Rv1038c esxJ Function unknown ESAT-6 like protein EsxJ (ESAT-6 like protein 2) Rv1038c, (MT1067, MTCY10G2.11), len: 98 aa. EsxJ, ESAT-6 like protein (see Gey Van Pittius et al., 2001), similar to Q49945|U1756C, Mycobacterium leprae (100 aa), FASTA scores: opt: 375, E(): 7.7e-21, (58.3% identity in 96 aa overlap). Member of M. tuberculosis hypothetical QILSS protein family with Rv1197, Rv1792, Rv2347c and Rv3620c. Belongs to the ESAT6 family. P9WNJ9 No cell wall and cell processes P9WNJ9 P9WNJ9 NC_000962.3 Mycobrowser_v5 CDS 1116531 1117148 . - 0 Rv1000c Rv1000c Function unknown Conserved hypothetical protein Rv1000c, len: 205 aa. Conserved hypothetical protein, equivalent to ML0190|NP_301263.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (205 aa). Also highly similar to SC5F8.12c|CAB93740.1|AL357613 hypothetical protein from Streptomyces coelicolor (210 aa), FASTA scores: E(): 2.1e-45, (56.8% identity); 9106290|AAF84108.1|AE003963_5|NP_298588.1|NC_002488 protein described as DNA repair system specific for alkylated DNA from Xylella fastidiosa (200 aa), FASTA scores: E(): 3.4e-14, (38.55% identity); and similar in C-terminus to other hypothetical proteins. Note that replaces original Rv1000 predicted on other strand. L7N6A4 No conserved hypotheticals L7N6A4 L7N6A4 NC_000962.3 Mycobrowser_v5 CDS 1117185 1118393 . + 0 Rv1001 arcA Arginine degradation [catalytic activity:L-arginine + H(2)O = L-citrulline + NH(3)] Probable arginine deiminase ArcA (adi) (ad) (arginine dihydrolase) Rv1001, (MTCI237.16), len: 402 aa. Probable arcA, arginine deiminase, similar to e.g. ARCA_PSEAE|P13981 arginine deiminase (417 aa), fasta scores: opt: 581, E(): 1.4e-31, (39.4% identity in 411 aa overlap); also similar to SAGP_STRPY|P16962 streptococcal acid glycoprotein (410 aa), FASTA scores, opt: 823, E():0, (38.3% identity in 402 aa overlap). Belongs to the arginine deiminase family. P9WQ05 No intermediary metabolism and respiration P63551 P9WQ05 GO:0016990,GO:0005737,GO:0006527 3.5.3.6 P9WQ05 NC_000962.3 Mycobrowser_v5 CDS 1118428 1119939 . - 0 Rv1002c Rv1002c Unknown Conserved membrane protein Rv1002c, (MTCI237.17c), len: 503 aa. Conserved membrane protein. Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). Similar to AL132674|SCE87.05 hypothetical protein from Streptomyces coelicolor (591 aa), FASTA scores: opt: 666, E(): 0, (39.0% identity in 546 aa overlap); weakly similar and to TSCC_PSEAM|P55019 thiazide-sensitive sodium-chloride cotransporter from Pseudopleuronectes americanus (1023 aa), FASTA scores: opt: 44, E(): 4.2e-06, (22.4% identity in 326 aa overlap). P9WN05 No cell wall and cell processes O05586 P9WN05 GO:0000030,GO:0005886,GO:0006493,GO:0016021 2.4.-.- NC_000962.3 Mycobrowser_v5 CDS 1120022 1120879 . + 0 Rv1003 Rv1003 Function unknown Conserved protein Rv1003, (MTCI237.19), len: 285 aa. Conserved protein, similar to others e.g. AL132674|SCE87.04 Streptomyces coelicolor (286 aa), FASTA scores: opt: 877, E(): 0, (53.2% identity in 280 aa overlap); and YRAL_ECOLI|P45528 hypothetical 31.3 kd protein (286 aa), FASTA scores: opt: 561, E(): 4.4e-27, (36.9% identity in 279 aa overlap). P9WGW7 No conserved hypotheticals P0A640 P9WGW7 GO:0008168,GO:0008152 2.1.1.- P9WGW7 NC_000962.3 Mycobrowser_v5 CDS 1120889 1122148 . - 0 Rv1004c Rv1004c Unknown Probable membrane protein Rv1004c, (MTCI237.20c), len: 419 aa. Probable membrane protein. Contains repetitive sequences, which have similarities with elastin, and possible N-terminal signal sequence. O05589 No cell wall and cell processes O05589 NC_000962.3 Mycobrowser_v5 CDS 1123714 1125417 . + 0 Rv1006 Rv1006 Unknown Unknown protein Rv1006, (MTCI237.23), len: 567 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). O05592 No conserved hypotheticals O05592 NC_000962.3 Mycobrowser_v5 CDS 1133921 1134568 . - 0 Rv1015c rplY Binds to the 50S rRNA 50S ribosomal protein L25 RplY Rv1015c, (MTCY10G2.34), len: 215 aa. rplY, 50s ribosomal protein L25, similar to RL25_ECOLI|P02426 50s ribosomal protein L25 from Escherichia coli (94 aa), FASTA scores: opt: 182, E(): 2.5e-05, (38.4% identity in 86 aa overlap) and to CTC_BACSU|P14194 general stress protein from Bacillus subtilis (203 aa), FASTA scores: opt: 260, E(): 1.4e-09, (28.4% identity in 201 aa overlap). Belongs to the L25P family of ribosomal proteins. P9WHB5 No information pathways P66121 P9WHB5 GO:0005840,GO:0003735,GO:0006412,GO:0008097 P9WHB5 NC_000962.3 Mycobrowser_v5 CDS 2139741 2140148 . + 0 Rv1891 Rv1891 Function unknown Conserved protein Rv1891, (MTCY180.27c), len: 135 aa. Conserved protein. Equivalent to MLCB561.09|AL049571 hypothetical protein from Mycobacterium leprae (134 aa), FASTA scores: opt: 800, E(): 0, (79.7% identity in 133 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O07741 No conserved hypotheticals O07741 NC_000962.3 Mycobrowser_v5 CDS 1128091 1129179 . + 0 Rv1009 rpfB Thought to promote the resuscitation and growth of dormant, nongrowing cells. Could also stimulate the growth of several other high G+C gram+ organisms, e.g. Mycobacterium avium, Mycobacterium bovis (BCG), Mycobacterium kansasii, Mycobacterium smegmatis. Probable resuscitation-promoting factor RpfB Rv1009, (MTCI237.26), len: 362 aa. Probable rpfB, resuscitation-promoting factor (see citation below), similar to others from Mycobacterium tuberculosis: Rv2450c|MTV008.06c|RPFE probable resuscitation-promoting factor (172 aa), FASTA scores: E(): 1.9e-19, (42.9% identity in 147 aa overlap); Rv0867c|RPFA, Rv1884c|RPFC, and Rv2389c|RPFD. Possible lipoprotein; contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Interacts with RipA (see Hett et al., 2007). Predicted possible vaccine candidate (See Zvi et al., 2008). Validated new TSS at 13 codons upstream, corresponding to the alternative TTG start (see Schwenk et. al 2018). A transcriptionally regulated RNA switch/riboswitch candidate was identified in the 5' UTR of rpfB (see Schwenk et. al 2018). P9WG29 No cell wall and cell processes 1XSF,3EO5 O05594 P9WG29 GO:0016787,GO:0005576 P9WG29 NC_000962.3 Mycobrowser_v5 CDS 1127089 1127883 . + 0 Rv1008 tatD DNase involved in protein export. This sec-independent pathway is termed tat for twin-arginine translocation system. This system mainly transports proteins with bound cofactors that require folding prior to export. Probable deoxyribonuclease TatD (YJJV protein) Rv1008, (MTCI237.25), len: 264 aa. Probable tatD (alternate gene name: yjjV), deoxyribonuclease, component of twin arginine translocation protein export system (see citations below). Similar to many members of the YBL055C/YJJV family e.g. YCFH_ECOLI|P37346 Putative deoxyribonuclease ycfH (265 aa), fasta scores: opt: 487, E(): 1.4e-24, (36.7% identity in 270 aa overlap). Also similar to P37545|YABD_BACSU Putative deoxyribonuclease yabD (255 aa), FASTA scores: opt: 599, E(): 7.7e-33, (40.1% identity in 262 aa overlap). Contains PS01137 Hypothetical YBL055c/yjjV family signature 1, and PS01091 Hypothetical YBL055c/yjjV family signature 3. O08343 No cell wall and cell processes O08343 O08343 GO:0016888 3.1.21.- O08343 NC_000962.3 Mycobrowser_v5 CDS 1129152 1130105 . + 0 Rv1010 ksgA Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. Its inactivation LEADS to kasugamycin resistance Probable dimethyladenosine transferase KsgA (S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase) (16S rRNA dimethylase) (high level kasugamycin resistance protein KsgA) (kasugamycin dimethyltransferase) Rv1010, (MTCI237.27), len: 317 aa. Probable ksgA, dimethyladenosine transferase, similar to many e.g. KSGA_BACSU|P37468 dimethyladenosine transferase from Bacillus subtilus (292 aa), FASTA scores: opt: 524, E(): 1.5e-28, (37.2% identity in 274 aa overlap); similar to Mycobacterium tuberculosis hypothetical protein Rv1988. Contains PS01131 Ribosomal RNA adenine dimethylases signature. P9WH07 No information pathways P66660 P9WH07 GO:0005737,GO:0000179,GO:0000154,GO:0003723 2.1.1.- P9WH07 NC_000962.3 Mycobrowser_v5 CDS 1130191 1131111 . + 0 Rv1011 ispE Thought to be involved in deoxyxylulose-5-phosphate pathway (DXP) of isoprenoid biosynthesis (at the fourth step). Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol [catalytic activity: ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol]. Probable 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase IspE (CMK) (4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase) Rv1011, (MTCI237.28, MT1040), len: 306 aa. Probable ispE, 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase , similar to others e.g. Q9K3R6|ISPE_STRCO Streptomyces coelicolor (299 aa), FASTA scores: opt: 925, E(): 2.7e-49, (54.5% identity in 297 overlap); etc. Belongs to the ISPE family. P9WKG7 No intermediary metabolism and respiration P65178 P9WKG7 GO:0005524,GO:0016310,GO:0016114,GO:0050515 2.7.1.148 P9WKG7 NC_000962.3 Mycobrowser_v5 CDS 1131128 1131421 . + 0 Rv1012 Rv1012 Unknown Hypothetical protein Rv1012, (MTCI237.29), len: 97 aa. Hypothetical unknown protein. O05597 No conserved hypotheticals O05597 NC_000962.3 Mycobrowser_v5 CDS 1995054 1995842 . - 0 Rv1762c Rv1762c Unknown Unknown protein Rv1762c, (MTCY28.28c), len: 262 aa. Unknown protein. O06797 No conserved hypotheticals O06797 O06797 NC_000962.3 Mycobrowser_v5 CDS 1133333 1133908 . - 0 Rv1014c pth The natural substrate for this enzyme may be peptidyl-TRNAS which drop off the ribosome during protein synthesis [catalytic activity: N-substituted aminoacyl-tRNA + H(2)O = N-substituted amino acid + tRNA] Probable peptidyl-tRNA hydrolase Pth Rv1014c, (MTCY10G2.35), len: 191 aa. Probable pth, peptidyl-tRNA hydrolase, similar to PTH_ECOLI|P23932 peptidy l-trna hydrolase from Escherichia coli (194 aa), FASTA scores: opt: 472, E(): 2.3e-25, (39.6% identity in 187 aa overlap). Belongs to the PTH family. P9WHN7 No intermediary metabolism and respiration 2JRC,2Z2I,2Z2J,2Z2K P65865 P9WHN7 GO:0005737,GO:0006412,GO:0004045 3.1.1.29 P9WHN7 NC_000962.3 Mycobrowser_v5 CDS 1214040 1214360 . + 0 Rv1087A Rv1087A Function unknown Conserved hypothetical protein Rv1087A, len: 106 aa (fragment). Conserved hypothetical protein, highly similar to C-terminus of near ORF O53434|YA86_MYCTU|Rv1086|MT1118|MTV017.39 short (C15) chain Z-isoprenyl diphosphate synthase from Mycobacterium tuberculosis (262 aa), FASTA scores: opt: 200, E(): 1.1e-06, (57.9% identity in 76 aa overlap). L7N654 No cell wall and cell processes Q8VK75 L7N654 GO:0016765 L7N654 NC_000962.3 Mycobrowser_v5 CDS 1135501 1136481 . - 0 Rv1017c prsA Catalyzes the formation of PRPP from ATP and ribose 5-phosphate. PRPP is then used in various biosynthetic pathways, as for example in the formation of purines, pyrimidines, histidine and tryptophan. [catalytic activity: ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate] Probable ribose-phosphate pyrophosphokinase PrsA (phosphoribosyl pyrophosphate synthetase) (PRPP synthetase) Rv1017c, (MTCY10G2.32), len: 326 aa. Probable prsA, ribose-phosphate pyrophosphokinase, highly similar to others e.g. KPRS_ECOLI|P08330 ribose-phosphate pyrophosphokinase from Escherichia coli (314 aa), FASTA scores: opt: 826, E(): 0, (43.8% identity in 317 aa overlap). Contains PS00103 Purine/pyrimidine phosphoribosyl transferases signature; contains PS00144 Asparaginase / glutaminase active site signature 1. Belongs to the ribose-phosphate pyrophosphokinase family. Cofactor: both inorganic phosphate and magnesium ion are required for enzyme stability and activity (by similarity). P9WKE3 No intermediary metabolism and respiration P65232 P9WKE3 GO:0005737,GO:0016301,GO:0000287,GO:0009116,GO:0009156,GO:0004749,GO:0005524 2.7.6.1 P9WKE3 NC_000962.3 Mycobrowser_v5 CDS 1136573 1138060 . - 0 Rv1018c glmU Peptidoglycan and lipopolysaccharide biosynthesis Probable UDP-N-acetylglucosamine pyrophosphorylase GlmU Rv1018c, (MTCY10G2.31), len: 495 aa. Probable glmU, UDP-n-acetylglucosamine pyrophosphorylase, similar to GCAD_BACSU|P14192 UDP-n-acetylglucosamine pyrophosphorylase (456 aa), FASTA scores: opt: 1150, E(): 0, (40.0% identity in 453 aa overlap). Similar to various Mycobacterium tuberculosis sugar-phosphate transferases e.g. Rv0334, Rv1213, Rv3264c, etc. P9WMN3 No cell wall and cell processes 2QKX,3D8V,3D98,3DJ4,3FOQ P96382 P9WMN3 GO:0000902,GO:0007047,GO:0005737,GO:0019134,GO:0009103,GO:0000287,GO:0009252,GO:0008360,GO:0003977 2.3.1.157,2.7.7.23 P9WMN3 NC_000962.3 Mycobrowser_v5 CDS 1138315 1138908 . + 0 Rv1019 Rv1019 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably TetR-family) Rv1019, (MTCY10G2.30c), len: 197 aa. Probable transcriptional regulator, similar to many memebers of the TetR family e.g. MTCY7D11.18c (34.4% identity in 189 aa overlap). Helix turn helix motif from aa 27-48 (+5.42 SD). P96381 No regulatory proteins P96381 P96381 GO:0006350,GO:0003700,GO:0006355 P96381 NC_000962.3 Mycobrowser_v5 CDS 1168704 1169228 . - 0 Rv1046c Rv1046c Unknown Hypothetical protein Rv1046c, (MTCY10G2.03), len: 174 aa. Hypothetical unknown protein. Start changed since first submission (-65 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L0T8G6 No unknown L0T8G6 NC_000962.3 Mycobrowser_v5 CDS 1341006 1341290 . + 0 Rv1198 esxL Unknown Putative ESAT-6 like protein EsxL (ESAT-6 like protein 4) Rv1198, (MT1236, MTCI364.10), len: 94 aa. EsxL, ESAT-6 like protein (see citation below). Member of the ESAT-6 family with Rv3619c, Rv1037c, etc. Almost identical to MTCY10G2.12 (94 aa) (97.9% identity in 94 aa overlap) and MTCY98.022c (94 aa) (94.7% identity in 94 aa overlap). Highly similar to Q49946|U1756D Mycobacterium leprae (95 aa), FASTA scores: opt: 403, E(): 1.1e-22, (64.1% identity in 92 aa overlap). seems to belong to the ESAT6 family. P9WNJ5 No cell wall and cell processes P9WNJ5 P9WNJ5 NC_000962.3 Mycobrowser_v5 CDS 1151920 1152012 . + 0 Rv1028A kdpF Thought to be involved in stabilization of the KDP complex. Probable membrane protein KdpF Rv1028A, len: 30 aa. Probable kdpF, membrane protein, showing similarity with P36937|KDPF_ECOLI|B0698.1 protein KDPF from Escherichia coli strain K12 (see citation below) (27% identity); and KdpF protein from Streptomyces coelicolor (51% identity). Q79FT7 No cell wall and cell processes Q79FT7 Q79FT7 NC_000962.3 Mycobrowser_v5 CDS 1159375 1160061 . - 0 Rv1035c Rv1035c Possibly required for the transposition of the insertion element IS1560. Probable transposase (fragment) Rv1035c, (MTCY10G2.14), len: 228 aa. Probable IS1560 transposase fragment, similar to parts of Rv3387|E1202305|MTV004.45 (225 aa) (47.8% identity in 67 aa overlap) and Rv3386|E1202304|MTV004.44 (234 aa) (55.1% identity in 127 aa overlap). P96366 No insertion seqs and phages P96366 NC_000962.3 Mycobrowser_v5 CDS 1165092 1165499 . - 0 Rv1042c Rv1042c Possibly required for the transposition of an insertion element. Probable is like-2 transposase Rv1042c, (MTCY10G2.07), len: 135 aa. Probable is like-2 transposase, similar to Q50761 transposase from Mycobacterium tuberculosis (308 aa), FASTA scores: opt: 823, E(): 0, (99.1% identity in 117 aa overlap). Second copy is Rv1149. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L0T897 No insertion seqs and phages L0T897 NC_000962.3 Mycobrowser_v5 CDS 1172881 1173786 . + 0 Rv1050 Rv1050 Function unknown; probably involved in cellular metabolism Probable oxidoreductase Rv1050, (MTV017.03), len: 301 aa. Probable oxidoreductase similar to many e.g. Rv1543|MTCY48.22C|Q10783 putative oxidoreductase CY48.22C (341 aa), FASTA scores: opt: 462, E(): 3e-22, (33.6% identity in 265 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53398 No intermediary metabolism and respiration O53398 O53398 GO:0055114,GO:0016491 1.-.-.- O53398 NC_000962.3 Mycobrowser_v5 CDS 1148427 1149107 . - 0 Rv1027c kdpE Member of the two-component regulatory system KDPD/KDPE involved in the regulation of the KDP operon. Probable transcriptional regulatory protein KdpE Rv1027c, (MTCY10G2.22), len: 226 aa. Probable KdpE, transcriptional regulatory protein, similar to others e.g. KDPE_ECOLI|P21866 kdp operon transcriptional regulatory protein from Escherichia coli strain K12 (225 aa), FASTA scores: opt: 691, E(): 0, (47.8% identity in 224 aa overlap); AL021530|SC2E9.13 from Streptomyces coelicolor (227 aa), FASTA scores: opt: 981, E(): 0, (66.4% identity in 226 aa overlap); etc. P9WGN1 No regulatory proteins P96373 P9WGN1 GO:0006355,GO:0006350,GO:0000156,GO:0000160,GO:0003677 P9WGN1 NC_000962.3 Mycobrowser_v5 CDS 1149104 1151686 . - 0 Rv1028c kdpD Member of the two-component regulatory system KDPD/KDPE involved in the regulation of the KDP operon. KDPD may function as a membrane-associated protein kinase that phosphorylates KDPE|Rv1027c in response to environmental signals. Probable sensor protein KdpD Rv1028c, (MTCY10G2.21), len: 860 aa. Probable kdpD, sensor protein, similar to others e.g. KDPD_ECOLI|P21865 sensor protein from Escherichia coli strain K12 (894 aa), FASTA scores: opt: 1041, E(): 0, (32.3% identity in 888 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to universal stress protein family. P9WGL3 No regulatory proteins P96372 P9WGL3 GO:0016021,GO:0018106,GO:0005886,GO:0006950,GO:0007165,GO:0000155,GO:0000160,GO:0005524 2.7.13.3 P9WGL3 NC_000962.3 Mycobrowser_v5 CDS 1155853 1156422 . + 0 Rv1031 kdpC One of the components of the high-affinity ATP-driven potassium transport (or KDP) system, which catalyzes the hydrolysis of ATP coupled with the exchange of hydrogen and potassium ions. The C subunit may be involved in assembly of the KDP complex. [catalytic activity: ATP + H(2)O + K(+)(out) = ADP + phosphate + K(+)(in)]. Probable potassium-transporting ATPase C chain KdpC (potassium-translocating ATPase C chain) (ATP phosphohydrolase [potassium-transporting] C chain) (potassium binding and translocating subunit C) Rv1031, (MTCY10G2.18c), len: 189 aa. Probable kdpC, potassium-transporting ATPase C chain (membrane protein) , similar to others e.g. ATKC_ECOLI|P03961 potassium-transporting ATPase C chain from Escherichia coli strain K12 (190 aa), FASTA scores: opt: 475, E(): 3.1e-24, (45.7% identity in 186 aa overlap); etc. Belongs to the KdpC family. P9WKF1 No cell wall and cell processes P65211 P9WKF1 GO:0016021,GO:0005886,GO:0006813,GO:0008556,GO:0005524 3.6.3.12 P9WKF1 NC_000962.3 Mycobrowser_v5 CDS 1161297 1162472 . - 0 Rv1039c PPE15 Function unknown PPE family protein PPE15 Rv1039c, (MTCY10G2.10), len: 391 aa. PPE15, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, most similar to Rv2768c|AL008967|MTV002_33 Mycobacterium tuberculosis H37Rv (394 aa), FASTA scores: opt: 1721, E(): 0, (70.4% identity in 398 aa overlap). P9WI31 No PE/PPE Q7D8Y7 P9WI31 P9WI31 NC_000962.3 Mycobrowser_v5 CDS 1172386 1172832 . + 0 Rv1049 Rv1049 Involved in transcriptional mechanism Probable transcriptional repressor protein Rv1049, (MTV017.02), len: 148 aa. Probable transcriptional repressor protein, similar to many e.g. P74870 negative regulator of EMR locus EMR from Salmonella typhimurium (149 aa), FASTA scores: opt: 146, E(): 0.0011, (31.6% identity in 95 aa overlap). Contains probable helix-turn-helix motif at aa 58-79 (Score 1495, +4.28 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6Y5H3 No regulatory proteins O53397 I6Y5H3 GO:0006355,GO:0006350,GO:0003700,GO:0005622 I6Y5H3 NC_000962.3 Mycobrowser_v5 CDS 1173945 1174700 . - 0 Rv1051c Rv1051c Function unknown Conserved hypothetical protein Rv1051c, (MTV017.04c), len: 251 aa. Conserved hypothetical protein, similar to LLU36837|U36837.1 protein encoded by Lactococcus lactis plasmid pNP40 (298 aa), FASTA scores: opt: 194, E(): 3.5e-06, (30.3% identity in 155 aa overlap). Contains possible helix-turn-helix motif at aa 197-218 (Score 1097, +2.92 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53399 No conserved hypotheticals O53399 O53399 GO:0000166 O53399 NC_000962.3 Mycobrowser_v5 CDS 1187435 1188001 . + 0 Rv1065 Rv1065 Function unknown Conserved hypothetical protein Rv1065, (MTV017.18), len: 188 aa. Conserved hypothetical protein, some similarity to AL0209|SC4H8_11 hypothetical protein from Streptomyces coelicolor (182 aa), FASTA scores: opt: 156, E(): 0.0011, (31.3% identity in 195 aa overlap). O53413 No conserved hypotheticals O53413 O53413 GO:0017172,GO:0005506,GO:0055114,GO:0046439 O53413 NC_000962.3 Mycobrowser_v5 CDS 1232311 1232826 . + 0 Rv1105 Rv1105 In combination with MTV017.57|Rv1104 catalyzes hydrolysis of several beta-lactam antibiotic PNB esters to the corresponding free acid and PNB alcohol Possible para-nitrobenzyl esterase (fragment) Rv1105, (MTV017.58), len: 171 aa. Possible para-nitrobenzyl esterase (fragment; possibly second part) . Similar to C-terminal domain of many e.g. P71048 para-nitrobenzyl esterase from Bacillus subtilis (489 aa), FASTA scores: opt: 248, E(): 2.7e-10, (32.3% identity in 167 aa overlap). Gene may be inactivated as a frameshift is required to obtain a product continuing from MTV017.57|Rv1104. Start changed since first submission. O53453 No intermediary metabolism and respiration O53453 O53453 GO:0016787 3.1.1.- O53453 NC_000962.3 Mycobrowser_v5 CDS 1156426 1157955 . - 0 Rv1032c trcS Sensor part of the two component regulatory system TRCS/TRCR. Two component sensor histidine kinase TrcS Rv1032c, (MTCY10G2.17), len: 509 aa. TrcS, two component sensor histidine kinase protein (see citations below), similar to YV16_MYCLE|P54883 probable sensor-like histidine kinase from Mycobacterium leprae (443 aa), FASTA scores: opt: 392, E(): 3.8e-18, (31.7% identity in 334 aa overlap). Note that in vitro autophosphorylation of TrcS requires the presence of Mn2+ or Ca2+ as a divalent cation cofactor and subsequent transphosphorylation of TrcR is evident in the presence of TrcS-phosphate and Ca2+. P96368 No regulatory proteins P96368 P96368 GO:0016021,GO:0018106,GO:0007165,GO:0000155,GO:0000160,GO:0005524 2.7.13.3 P96368 NC_000962.3 Mycobrowser_v5 CDS 1160544 1160828 . - 0 Rv1037c esxI Unknown Putative ESAT-6 like protein EsxI (ESAT-6 like protein 1) Rv1037c, (MTCY10G2.12), len: 94 aa. EsxI, ESAT-6 like protein (see citations below), highly similar to Q49946|ES6X_MYCLE|U1756D putative ESAT-6 like protein X from Mycobacterium leprae (95 aa), FASTA scores: opt: 409, E(): 6.3e-23, (64.15% identity in 92 aa overlap); Rv3619c, Rv1198, Rv2346c, etc from Mycobacterium tuberculosis. Strictly identical to P96364|ES61_MYCTU|Rv3619c|MTCY15C10.33|MTCY07H7B.03|MT3721 putative ESAT-6 like protein 1 (94 aa). Belongs to the ESAT6 family. P0DOA6 No cell wall and cell processes P0DOA6 P0DOA6 NC_000962.3 Mycobrowser_v5 CDS 1164572 1165435 . - 0 Rv1041c Rv1041c Possibly required for the transposition of an insertion element. Probable is like-2 transposase Rv1041c, (MTCY10G2.08), len: 287 aa. Probable is like-2 transposase, overlaps MTCY10G2.07. Similar to Q00430|X53945 insertion element IS869 hypothetical protein from Agrobacterium tumefaciens (186 aa), FASTA scores: opt: 173, E(): 0.00016, (40.9% identity in 176 aa overlap). Similar to Rv1150, C-terminal part of transposase of putative Mycobacterium tuberculosis is like-1. MTCY10G2.07 and MTCY10G2.08 are frameshifted with respect to Mycobacterium tuberculosis Q50761 transposase, the 10G2 cosmid sequence appears to be correct. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P96360 No insertion seqs and phages P96360 P96360 GO:0004803,GO:0006313,GO:0003677 P96360 NC_000962.3 Mycobrowser_v5 CDS 1165781 1166806 . - 0 Rv1043c Rv1043c Function unknown Conserved hypothetical protein Rv1043c, (MTCY10G2.06), len: 341 aa. Conserved hypothetical protein similar to AL096872|SC5F7.08 putative lipoate-protein ligase from Streptomyces coelicolor (362 aa), FASTA scores: opt: 206, E(): 1.4e-05, (30.3% identity in 201 aa overlap). Weak similarity to P39668|YYXA_BACSU hypothetical protease from Bacillus subtitis (400 aa), FASTA scores: opt: 159, E(): 0.013, (27.1% identity in 210 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P96358 No conserved hypotheticals P96358 P96358 GO:0004252,GO:0006508 P96358 NC_000962.3 Mycobrowser_v5 CDS 1167053 1167676 . + 0 Rv1044 Rv1044 Function unknown Conserved hypothetical protein Rv1044, (MTCY10G2.05c), len: 207 aa. Conserved hypothetical protein, similar to Mycobacterium tuberculosis hypothetical protein MTCY06G11.02C|P96837 (289 aa), fasta scores: E(): 8.9e-06, (30.7% identity in 150 aa overlap). Some similarity to U36837|LLU36837_1 Lactococcus lactis plasmid pNP40 (287 aa), FASTA scores: opt: 147, E (): 0.0087, (29.7% identity in 91 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P96357 No conserved hypotheticals P96357 P96357 NC_000962.3 Mycobrowser_v5 CDS 1167673 1168554 . + 0 Rv1045 Rv1045 Unknown Hypothetical protein Rv1045, (MTCY10G2.04c), len: 293 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P96356 No conserved hypotheticals P96356 P96356 P96356 NC_000962.3 Mycobrowser_v5 CDS 1169423 1170670 . + 0 Rv1047 Rv1047 Possibly required for the transposition of the insertion element IS1081. Probable transposase Rv1047, (MTCY10G2.02c), len: 415 aa. IS1081 transposase, most similar to TRA1_MYCBO|P35882 transposase for insertion sequence element (415 aa), FASTA scores: opt: 2675, E(): 0, (99.8% identity in 415 aa overlap). Contains PS01007 Transposases, Mutator family, signature. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P96354 No insertion seqs and phages P96354 P96354 GO:0004803,GO:0006313,GO:0003677 P96354 NC_000962.3 Mycobrowser_v5 CDS 1171038 1172153 . - 0 Rv1048c Rv1048c Unknown Hypothetical protein Rv1048c, (MTV017.01c-MTCY10G2.01), len: 371 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P96353 No conserved hypotheticals P96353 P96353 NC_000962.3 Mycobrowser_v5 CDS 1187998 1188393 . + 0 Rv1066 Rv1066 Function unknown Conserved hypothetical protein Rv1066, (MTV017.19), len: 131 aa. Conserved hypothetical protein, strong similarity to AL0209|SC4H8.10 hypothetical protein from Streptomyces coelicolor (132 aa), FASTA scores: opt: 429, E(): 5.2e-23, (57.1% identity in 119 aa overlap). O53414 No conserved hypotheticals O53414 O53414 O53414 NC_000962.3 Mycobrowser_v5 CDS 1177239 1177373 . + 0 Rv1055 Rv1055 Use for sequence integration. Integrase is necessary for integration of a phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome. Possible integrase (fragment) Rv1055, (MTV017.08), len: 44 aa. Possible integrase (fragment); first 49 aa similar to Rv2309c|MTCY3G12_25|Z79702 hypothetical protein (shows similarity to integrases) from Mycobacterium tuberculosis (151 aa), FASTA scores: opt: 291, E(): 2.2e-16, (74.3% identity in 70 aa overlap); and to L39071|MSGINT_1 integrase from Mycobacterium paratuberculosis (191 aa), FASTA scores: opt: 146, E(): 8.3e-05, (52.1% identity in 48 aa overlap); and to many other integrases or transposases. Shortened since first submission (-34 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53403 No insertion seqs and phages O53403 NC_000962.3 Mycobrowser_v5 CDS 1177628 1178392 . + 0 Rv1056 Rv1056 Function unknown Conserved protein Rv1056, (MTV017.09), len: 254 aa. Conserved protein, some similarity in C-terminal region of Rv0140|MTCI5.14|Z92770 Mycobacterium tuberculosis (126 aa), FASTA scores: opt: 254, E(): 1.2e-10, (43.4% identity in 106 aa overlap); and to Rv1670. C-terminal region is similar to AL035569|SC8D9.02 hypothetical protein from Streptomyces coelicolor (113 aa), FASTA scores: opt: 282, E(): 4.5e-12, (48.0% identity in 100 aa overlap). O53404 No conserved hypotheticals O53404 O53404 O53404 NC_000962.3 Mycobrowser_v5 CDS 1180684 1182315 . + 0 Rv1058 fadD14 Involved in the fatty acid beta oxidation pathway (degradation). Probable medium chain fatty-acid-CoA ligase FadD14 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv1058, (MTV017.11), len: 543 aa. Probable fadD14, medium-chain fatty-acid-CoA synthetase, highly similar to many e.g. CAC32346.1|AL583945 putative fatty acid CoA ligase from Streptomyces coelicolor (558 aa); N-terminus of NP_419738.1|NC_002696 medium-chain-fatty-acid--CoA ligase from Caulobacter crescentus (1006 aa); Q00594|ALKK_PSEOL medium-chain-fatty-acid--CoA ligase from Pseudomonas oleovorans (546 aa), FASTA scores: opt: 1468, E(): 0, (41.1% identity in 538 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family. O53406 No lipid metabolism O53406 O53406 GO:0008152,GO:0016874 6.2.1.- O53406 NC_000962.3 Mycobrowser_v5 CDS 1182391 1183455 . + 0 Rv1059 Rv1059 Function unknown Conserved protein Rv1059, (MTV017.12), len: 354 aa. Conserved protein, similar to Rv0926c|MTCY21C12.20c hypothetical protein from Mycobacterium tuberculosis (358 aa), FASTA scores: opt: 338, E(): 1.4e-14, (33.1% identity in 363 aa overlap). O53407 No conserved hypotheticals O53407 O53407 GO:0008839,GO:0009089,GO:0055114 NC_000962.3 Mycobrowser_v5 CDS 1231301 1231990 . + 0 Rv1104 Rv1104 In combination with MTV017.58|Rv1105 catalyzes hydrolysis of several beta-lactam antibiotic PNB esters to the corresponding free acid and PNB alcohol Possible para-nitrobenzyl esterase (fragment) Rv1104, (MTV017.57), len: 229 aa. Possible para-nitrobenzyl esterase (fragment; possibly first part) . Similar to the N-terminal domain of many e.g. P37967|PNBA_BACSU Bacillus subtilis (489 aa), FASTA scores: opt: 715, E(): 0, (53.4% identity in 191 aa overlap). Gene may be inactivated as a frameshift is required to obtain a product continuing in MTV017.58|Rv1105. O53452 No intermediary metabolism and respiration O53452 O53452 GO:0016787 3.1.1.- O53452 NC_000962.3 Mycobrowser_v5 CDS 2000074 2000433 . + 0 Rv1767 Rv1767 Function unknown Conserved protein Rv1767, (MTCY28.33), len: 119 aa. Conserved protein, similar to Q57498|YA53_HAEIN hypothetical protein HI1053 from Haemophilus influenzae (113 aa), FASTA scores: opt: 233, E(): 6.4e-10, (40.0% identity in 90 aa overlap). O06800 No conserved hypotheticals O06800 O06800 O06800 NC_000962.3 Mycobrowser_v5 CDS 1186904 1187323 . - 0 Rv1064c lpqV Unknown Possible lipoprotein LpqV Rv1064c, (MTV017.17c), len: 139 aa. Possible lipoprotein LpqV. Has N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. P9WK57 No cell wall and cell processes P9WK57 GO:0005886 NC_000962.3 Mycobrowser_v5 CDS 1190757 1192148 . - 0 Rv1068c PE_PGRS20 Function unknown PE-PGRS family protein PE_PGRS20 Rv1068c, (MTV017.21c), len: 463 aa. PE_PGRS20, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002). Similar to AL021897|MTV017_19 Mycobacterium tuberculosis H37Rv (667 aa), FASTA scores: opt: 1875, E(): 0, (55.0% identity in 667 aa overlap). P9WIF9 No PE/PPE O53416 P9WIF9 P9WIF9 NC_000962.3 Mycobrowser_v5 CDS 1696727 1697422 . - 0 Rv1507c Rv1507c Function unknown Conserved protein Rv1507c, (MTCY277.29c), len: 231 aa. Conserved protein. Similar to AJ007747|BBR007747_6 Hypothetical protein BbLPS1.06 from Bordetella bronchiseptica cosmid (239 aa), FASTA scores: opt: 362, E(): 1.3e-17, (30.8% identity in 221 aa overlap). P9WLW5 No conserved hypotheticals P71786 P9WLW5 P9WLW5 NC_000962.3 Mycobrowser_v5 CDS 1192510 1194273 . - 0 Rv1069c Rv1069c Function unknown Conserved protein Rv1069c, (MTV017.22c), len: 587 aa. Conserved protein, hydrophobic regions in N-terminal domain. Similar in part to O07136|B1306.04C B1306.04c protein from Mycobacterium leprae (89 aa), FASTA scores: opt: 229, E(): 1.3e-07, (54.2% identity in 72 aa overlap). O53417 No conserved hypotheticals O53417 O53417 O53417 NC_000962.3 Mycobrowser_v5 CDS 1194270 1195043 . - 0 Rv1070c echA8 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Probable enoyl-CoA hydratase EchA8 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv1070c, (MTV017.23c), len: 257 aa. Probable echA8, enoyl-CoA hydratase, equivalent to O07137|B1306.05c putative enoyl-CoA hydratase/isomerase from Mycobacterium leprae (257 aa), FASTA scores: opt: 1417, E(): 0, (86.4% identity in 257 aa overlap). Also highly similar to others e.g. NP_106219.1|NC_002678 enoyl CoA hydratase from Mesorhizobium loti (257 aa); L39265|RHMRPST_2 enoyl-CoA hydratase from Rhizobium melilotii (257 aa), FASTA scores: opt: 1100, E(): 0, (66.9% identity in 257 aa overlap); AAK18173.1|AF290950_5|AF290950|FadB1x enoyl-CoA hydratase from Pseudomonas putida (257 aa); etc. Contains PS00166 Enoyl-CoA hydratase/isomerase signature. Belongs to the enoyl-CoA hydratase/isomerase family. P9WNN9 No lipid metabolism 3H81,3PZK P64016 P9WNN9 GO:0006631,GO:0004300 4.2.1.17 P9WNN9 NC_000962.3 Mycobrowser_v5 CDS 1228683 1229384 . + 0 Rv1100 Rv1100 Function unknown Conserved protein Rv1100, (MTV017.53), len: 233 aa. Conserved protein, slightly similar to Rv1906c|MTCY180.12 hypothetical protein from Mycobacterium tuberculosis (156 aa), FASTA scores: opt: 122, E(): 6.9, (27.4% identity in 135 aa overlap). Equivalent to AL049491|MLCB1222_9 Mycobacterium leprae (257 aa) (63.8% identity in 257 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53448 No conserved hypotheticals O53448 NC_000962.3 Mycobrowser_v5 CDS 1196279 1197115 . + 0 Rv1072 Rv1072 Unknown Probable conserved transmembrane protein Rv1072, (MTV017.25), len: 278 aa. Probable conserved transmembrane protein, equivalent to O07139|B1306.07|Y13803 Protein B1306.07 from Mycobacterium leprae (220 aa), FASTA scores: opt:1032, E(): 0, (75.0% identity in 220 aa overlap); and at the C-terminal end to Q50056|U1740D Mycobacterium leprae (96 aa), FASTA scores: opt: 381, E(): 1.2e-18, (71.6% identity in 81 aa overlap). Similar to Q54192|M80628|STMBLDA_1 transfer RNA-LEU (BLDA) gene and ORF from Streptomyces griseus (293 aa), FASTA scores: opt:558, E(): 4.7e-30, (41.5% identity in 299 aa overlap). O53420 No cell wall and cell processes O53420 O53420 GO:0016021 O53420 NC_000962.3 Mycobrowser_v5 CDS 1199426 1200370 . - 0 Rv1075c Rv1075c Function unknown Conserved exported protein Rv1075c, (MTV017.28c), len: 314 aa. Possibly exported protein, as it contains a N-terminal signal sequence, hydrophobic domain from aa 7-25. Similar to U15183|MLU15183_2 Mycobacterium leprae cosmid B1740 (106 aa), FASTA scores: opt: 207, E(): 1.6e-06, (42.6% identity in 101 aa overlap). Also weak similarity to many glyceraldehyde-3-phosphate dehydrogenases e.g. Q41595|G3PC_TAXBA Taxus baccata (340 aa), FASTA scores: opt: 147, E(): 0.027, (27.5% identity in 189 aa overlap). O53423 No cell wall and cell processes O53423 O53423 GO:0006629,GO:0016788 O53423 NC_000962.3 Mycobrowser_v5 CDS 1200767 1201660 . + 0 Rv1076 lipU Hydrolyses lipids Possible lipase LipU Rv1076, (MTV017.29), len: 297 aa. Possible lipU, lipase, very similar to several Mycobacterium tuberculosis proteins e.g. Z95390|Rv3487c|MTCY13E12.41c (277 aa), FASTA scores: opt: 1225, E(): 0, (76.0% identity in 246 aa overlap); Rv1426c, etc. Also similar to esterases and lipases of around 300 aa e.g. Q44087 esterase precursor from Acinetobacter lwoffii esterase (303), FASTA scores: opt: 427, E(): 1.9e-21, (32.5% identity in 280 aa overlap). Equivalent to AL035159|MLCB1450 _7 Mycobacterium leprae (335 aa), FASTA scores: opt: 1588, E(): 0, (79.7% identity in 296 aa overlap). O53424 No intermediary metabolism and respiration O53424 O53424 GO:0008152,GO:0016787 3.1.-.- O53424 NC_000962.3 Mycobrowser_v5 CDS 1203313 1204035 . + 0 Rv1078 pra Unknown Probable proline-rich antigen homolog Pra Rv1078, (MTV017.31), len: 240 aa. Probable pra, Proline-rich antigen homolog, equivalent to X65546|MLPRAG_1 proline rich antigen from Mycobacterium leprae (249 aa), FASTA scores: opt: 1162, E(): 3.3e-30, (64.8% identity in 253 aa overlap). Has potential hydrophobic domains. P9WIM7 No conserved hypotheticals O53426 P9WIM7 NC_000962.3 Mycobrowser_v5 CDS 1229391 1230548 . - 0 Rv1101c Rv1101c Unknown Conserved membrane protein Rv1101c, (MTV017.54c), len: 385 aa. Conserved membrane protein, shows some similarity to other bacterial proteins e.g. P77406|PERM_ECOLI putative permease perm from Escherichia coli (353 aa), FASTA scores: opt: 287, E(): 8.8e-12, (24.9% identity in 349 aa overlap). P9WFM3 No cell wall and cell processes O53449 P9WFM3 GO:0005886,GO:0016021 P9WFM3 NC_000962.3 Mycobrowser_v5 CDS 1205304 1205798 . - 0 Rv1080c greA Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent trancript by cleavage factors such as GREA or GREB allows the resumption of elongation from the new 3'terminus. GREA releases sequences of 2 to 3 nucleotides Probable transcription elongation factor GreA (transcript cleavage factor GreA) Rv1080c, (MTV017.33c), len: 164 aa. Probable greA, transcription elongation factor G, closest to P46808|GREA_MYCLE transcription elongation factor G from Mycobacterium leprae (202 aa), FASTA scores: opt: 1005, E(): 0, (94.5% identity in 164 aa overlap); and similar to many e.g. P21346|GREA_ECOLI from Escherichia coli (158 aa), FASTA scores: opt: 257, E(): 5.7e-10, (37.2% identity in 148 aa overlap); etc. Contains two PS00829 and one PS00830 Prokaryotic transcription elongation factors signatures 1 and 2, respectively. Belongs to the GREA/GREB family. P9WMT9 No information pathways P64279 P9WMT9 GO:0006355,GO:0006350,GO:0003711,GO:0003677 P9WMT9 NC_000962.3 Mycobrowser_v5 CDS 1205984 1206418 . - 0 Rv1081c Rv1081c Unknown Probable conserved membrane protein Rv1081c, (MTV017.34c), len: 144 aa. Probable conserved membrane protein, with hydrophobic stretch from aa 26 - 48, highly similar to NP_302548.1|NC_002677 conserved membrane protein from Mycobacterium leprae. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). O53429 No cell wall and cell processes O53429 NC_000962.3 Mycobrowser_v5 CDS 1207383 1207649 . + 0 Rv1083 Rv1083 Function unknown Conserved hypothetical protein Rv1083, (MTV017.36), len: 88 aa. Conserved hypothetical protein, similar to U15183|MLU15183_9 hypothetical protein from Mycobacterium leprae (167 aa), FASTA scores: opt: 332, E(): 1.2e-13, (58.4% identity in 101 aa overlap). Hydrophobic domain aa 25-43. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53431 No conserved hypotheticals O53431 NC_000962.3 Mycobrowser_v5 CDS 1219248 1220186 . - 0 Rv1092c coaA Coenzyme A (CoA) biosynthesis [catalytic activity: ATP + pantothenate = ADP + D-4'- phosphopantothenate.] Probable pantothenate kinase CoaA (pantothenic acid kinase) Rv1092c, (MTV017.45c), len: 312 aa. Probable coaA, pantothenate kinase, similar to many e.g. P15044|COAA_ECOLI Escherichia coli (316 aa), FASTA scores :opt: 1079, E(): 0, (52.7% identity in 311 aa overlap). Equivalent to AL049491|MLCB1222_17 Mycobacterium leprae (312 aa) (93.6% identity in 312 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the pantothenate kinase family. P9WPA7 No intermediary metabolism and respiration 2GES,2GET,2GEU,2GEV,2ZS7,2ZS8,2ZS9,2ZSA,2ZSB,2ZSD,2ZSE,2ZSF,3AEZ,3AF0,3AF1,3AF2,3AF3,3AF4,3AVO,3AVP,3AVQ P63810 P9WPA7 GO:0015937,GO:0005737,GO:0004594,GO:0005524 2.7.1.33 P9WPA7 NC_000962.3 Mycobrowser_v5 CDS 1207636 1209657 . + 0 Rv1084 Rv1084 Function unknown Conserved protein Rv1084, (MTV017.37), len: 673 aa. Conserved protein, similar to P37512|YYAL_BACSU hypothetical protein from Bacillus subtilis (689 aa), FASTA scores: opt: 1063, E() : 0, (36.5% identity in 696 aa overlap); AE0009|AE000983_10 Archaeoglobus fulgidus section 1 (642 aa), FASTA scores: opt: 1018, E(): 0, (37.2% identity in 600 aa overlap). Also similar to AE001938|AE001938_9 Deinococcus radiodurans (690 aa), FASTA scores: opt: 1097, E(): 0, (41.6% identity in 694 aa overlap). O53432 No conserved hypotheticals O53432 O53432 GO:0003824 O53432 NC_000962.3 Mycobrowser_v5 CDS 1209756 1210484 . - 0 Rv1085c Rv1085c Unknown, but supposedly involved in virulence Possible hemolysin-like protein Rv1085c, (MTV017.38c), len: 242 aa. Possible hemolysin-like protein, integral membrane protein, similar to many hemolysins, and hypothetical proteins e.g. U28375|ECU28375_49 Hypothetical protein from Escherichia coli (219 aa), FASTA scores: opt: 308, E(): 7.5e-15, (30.6% identity in 180 aa overlap); AE0011|HIAE001124_2 Hypothetical protein from Borrelia burgdorferi (233 aa), FASTA scores: opt: 305, E(): 1.3e-14, (25.6% identity in 203 aa overlap). Also weakly similar to HLY3_BACCE|P54176 haemolysin from Bacillus cereus (219 aa), FASTA scores: opt: 247, E(): 8.7e-12, (27.5% identity in 171 aa overlap). Also similar to AE002027|AE002027_8 probable hemolysin from Deinococcus radiodurans (219 aa), FASTA scores: opt: 354, E(): 1.8e-16, (31.1% identity in 219 aa overlap). P9WFN7 No virulence, detoxification, adaptation P67157 P9WFN7 GO:0016021,GO:0005886,GO:0019835 NC_000962.3 Mycobrowser_v5 CDS 1220574 1221854 . + 0 Rv1093 glyA1 Interconversion of serine and glycine. Key enzyme in the biosynthesis of purines, lipids, hormones and other components [catalytic activity: 5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine.] Serine hydroxymethyltransferase 1 GlyA1 Rv1093, (MTV017.46), len: 426 aa. glyA1, serine hydroxymethyltransferase 1, equivalent to AL049491|MLCB1222_16 from Mycobacterium leprae (426 aa), FASTA score: (89.9 % identity in 426 aa overlap). Also similar to many e.g. P34895|GLYA_HYPME hyphomicrobium methylovorum (434 aa), FASTA scores: opt: 1492, E(): 0, (56.8% identity in 419 aa overlap); etc. Belongs to the ShmT family. Note that previously known as glyA. P9WGI9 No intermediary metabolism and respiration 1LXB,3H7F O53441 P9WGI9 GO:0005737,GO:0004372,GO:0006544,GO:0006730,GO:0030170,GO:0006563 2.1.2.1 P9WGI9 NC_000962.3 Mycobrowser_v5 CDS 1214769 1215131 . + 0 Rv1089 PE10 Function unknown PE family protein PE10 Rv1089, (MTV017.42), len: 120 aa. PE10, Member of the Mycobacterium tuberculosis PE family of glycine-rich proteins (see citation below). Partial ORF that appears to be frameshifted continuation of Rv1088|MTV017.41. Sequence has been checked and appears correct. Similar to Z95555|MTCY06F7_4 Mycobacterium tuberculosis cosmid (401 aa), FASTA scores: opt:126, E(): 2, (29.6% identity in 125 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L0T5T4 No PE/PPE L0T5T4 NC_000962.3 Mycobrowser_v5 CDS 1215517 1215621 . + 0 Rv1089A celA2a The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) exocellobiohydrolases that cut the dissaccharide cellobiose from the nonreducing end of the cellulose polymer chain; (3) beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose [catalytic activity:endohydrolysis of 1,4-beta-D-glucosidic linkages in cellulose]. Probable cellulase CelA2a (endo-1,4-beta-glucanase) (endoglucanase) (carboxymethyl cellulase) Rv1089A, len: 34 aa. Probable celA2a, first part of cellulase (endoglucanase), similar to N-terminus of others. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q79FS6 No intermediary metabolism and respiration Q79FS6 GO:0008152,GO:0008810 3.2.1.4 Q79FS6 NC_000962.3 Mycobrowser_v5 CDS 1215599 1216054 . + 0 Rv1090 celA2b The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) exocellobiohydrolases that cut the dissaccharide cellobiose from the nonreducing end of the cellulose polymer chain; (3) beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose [catalytic activity:endohydrolysis of 1,4-beta-D-glucosidic linkages in cellulose]. Probable cellulase CelA2b (endo-1,4-beta-glucanase) (endoglucanase) (carboxymethyl cellulase) Rv1090, (MTV017.43), len: 151 aa. Probable celA2b, second part of cellulase (endoglucanase), similar to C-terminus of others e.g. O08468 cellulase CEL2 from Streptomyces halstedi (377 aa), FASTA scores: opt: 554, E(): 1.2e-30, (52.0% identity in 152 aa overlap); etc. Gene appears to have been inactivated by frameshift mutations but no errors could be found that would account for this. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53438 No intermediary metabolism and respiration O53438 O53438 GO:0000272,GO:0008810 3.2.1.4 O53438 NC_000962.3 Mycobrowser_v5 CDS 1216469 1219030 . + 0 Rv1091 PE_PGRS22 Function unknown PE-PGRS family protein PE_PGRS22 Rv1091, (MTV017.44), len: 853 aa. PE_PGRS22, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below). Similar to Rv1087|AL021897|MTV017_39 Mycobacterium tuberculosis H37Rv (767 aa), FASTA scores: opt: 2819, E(): 0, (60.0% identity in 860 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q79FS5 No PE/PPE Q79FS5 Q79FS5 Q79FS5 NC_000962.3 Mycobrowser_v5 CDS 1230660 1230971 . - 0 Rv1102c mazF3 Sequence-specific mRNA cleavage Toxin MazF3 Rv1102c, (MTV017.55c), len: 103 aa. MazF3, toxin, part of toxin-antitoxin (TA) operon with Rv1103c (See Pandey and Gerdes, 2005; Zhu et al., 2006), similar to Mycobacterium tuberculosis hypothetical protens e.g. Rv1942c|MTCY9F9_22 (109 aa), FASTA scores: opt: 158, E(): 3.6e-05, (33.3% identity in 93 aa overlap); Rv0659c|MTCI376_17 (102aa), opt: 140, E(): 0.00072, (40.6% identity in 69aa overlap); and Rv1495. P9WIH9 No virulence, detoxification, adaptation O53450 P9WIH9 GO:0003677 P9WIH9 NC_000962.3 Mycobrowser_v5 CDS 2140165 2140476 . + 0 Rv1892 Rv1892 Unknown Probable membrane protein Rv1892, (MTCY180.26c), len: 103 aa. Probable membrane protein. O07740 No cell wall and cell processes O07740 O07740 NC_000962.3 Mycobrowser_v5 CDS 1222997 1224298 . + 0 Rv1095 phoH2 Unknown Probable PHOH-like protein PhoH2 (phosphate starvation-inducible protein PSIH) Rv1095, (MTV017.48), len: 433 aa. Probable phoH2, phoH-like protein (phosphate starvation-induced protein), probably ATP-binding protein. Equivalent to AL049491 MLCB1222_14 Mycobacterium leprae (433 aa) (92.8% identity in 432 aa overlap). Similar to many proteins described as PhoH-like e.g. Z97025|BSZ97025_12 Bacillus subtilis (442 aa), FASTA scores: opt: 605, E(): 0, (40.1% identity in 444 aa overlap); or Mycobacterium tuberculosis Rv2368c|O05830|PHOL_MYCTU Mycobacterium tuberculosis (352 aa), FASTA scores: opt: 390, E(): 4e-19, (31.5% identity in 241 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the PhoH family. O53443 No intermediary metabolism and respiration O53443 O53443 GO:0005524 O53443 NC_000962.3 Mycobrowser_v5 CDS 2156706 2157299 . - 0 Rv1910c Rv1910c Unknown Probable exported protein Rv1910c, (MTCY180.08), len: 197 aa. Possible exported protein, very similar to upstream ORF MTCY180.07 (201 aa), FASTA score: E(): 0, (64.0% identity in 200 aa overlap). Also similar to Q9Z729|Y877_CHLPN protein CPN0877 from Chlamydophila pneumoniae (150 aa). Predicted to be an outer membrane protein (See Song et al., 2008). P9WFN5 No cell wall and cell processes P67222 P9WFN5 P9WFN5 NC_000962.3 Mycobrowser_v5 CDS 1224385 1225260 . + 0 Rv1096 Rv1096 Probably involved in carbohydrate degradation. May hydrolyse the glycosidic bond between two or more carbohydrates or between a carbohydrate and a non-carbohydrate moiety. Possible glycosyl hydrolase Rv1096, (MTV017.49), len: 291 aa. Possible glycosyl hydrolase, possibly deacetylase or esterase. Equivalent to AL049491|MLCB1222_13 Mycobacterium leprae (291 aa) (81.3% identity in 289 aa overlap). Similar at the C-terminus to enzymes involved in carbohydrate degradation including Z99110|BSUB0007_92 endo-1,4-beta-xylanase homolog yjeA from Bacillus subtilis (467 aa), FASTA scores: opt: 418, E(): 2.6e-17, (38.6% identity in 184 aa overlap); M64552|STMXLNB_2 acetyl-xylan esterase from Streptomyces lividans (335 aa), FASTA scores: opt: 371, E(): 1.1e-14, (31.6% identity in 237 aa overlap); NP_345933.1|NC_003028 peptidoglycan N-acetylglucosamine deacetylase a from Streptococcus pneumoniae (463 aa); etc. Has possible N-terminal signal sequence with TMhelix at aa 13-31. O53444 No intermediary metabolism and respiration O53444 O53444 GO:0016810,GO:0005975 3.-.-.- O53444 NC_000962.3 Mycobrowser_v5 CDS 1230971 1231291 . - 0 Rv1103c mazE3 Function unknown Possible antitoxin MazE3 Rv1103c, (MTV017.56c), len: 106 aa. Possible mazE3, antitoxin, part of toxin-antitoxin (TA) operon with Rv1102c (See Pandey and Gerdes, 2005; Zhu et al., 2006). Note that Zhu et al., 2006 identifies a different amino acid sequence as the possible antitoxin to Rv1102c. Similar to part of Mycobacterium tuberculosis hypothetical protein Rv2472|AL021246|MTV008_27 Mycobacterium tuberculosis (97 aa), FASTA score: opt: 135, E(): 0.0091, (45.8% identity in 72 aa overlap). O53451 No virulence, detoxification, adaptation O53451 NC_000962.3 Mycobrowser_v5 CDS 1235457 1236095 . - 0 Rv1109c Rv1109c Function unknown Conserved protein Rv1109c, (MTV017.62c), len: 212 aa. Conserved protein. Equivalent to AL049491|MLCB1222_4 hypothetical protein from Mycobacterium leprae (205 aa) (68.1% identity in 213 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WM59 No conserved hypotheticals P9WM59 NC_000962.3 Mycobrowser_v5 CDS 1239416 1239613 . + 0 Rv1113 vapB32 Unknown Possible antitoxin VapB32 Rv1113, (MTCY22G8.02), len: 65 aa. Possible vapB32, antitoxin, part of toxin-antitoxin (TA) operon with Rv1114, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. Rv2758c|AL00896 7|MTV002.23 (88 aa) FASTA scores: opt: 97, E(): 0.86, (33.3% identity in 69 aa overlap). Part of family including Rv2871, Rv1241, Rv2132, Rv3321c, etc. P9WJ33 No virulence, detoxification, adaptation O06565 P9WJ33 P9WJ33 NC_000962.3 Mycobrowser_v5 CDS 1239610 1239984 . + 0 Rv1114 vapC32 Unknown Possible toxin VapC32. Contains PIN domain. Rv1114, (MTCY22G8.03), len: 124 aa. Possible vapC32, toxin, part of toxin-antitoxin (TA) operon with Rv1113, contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. Rv1561 and Rv2010. P9WF73 No virulence, detoxification, adaptation O06566 P9WF73 P9WF73 NC_000962.3 Mycobrowser_v5 CDS 1253074 1254534 . - 0 Rv1129c Rv1129c Involved in transcriptional mechanism Probable transcriptional regulator protein Rv1129c, (MTCY22G8.18c), len: 486 aa. Possible transcriptional regulator protein, similar to Rv0465c|MTV038.09c Mycobacterium tuberculosis (474 aa), FASTA scores: E(): 0, (47.4% identity in 468 aa overlap). Helix turn helix motif present from aa 32-53. O06581 No regulatory proteins O06581 O06581 GO:0043565 O06581 NC_000962.3 Mycobrowser_v5 CDS 1227596 1228684 . - 0 Rv1099c glpX Involved in gluconeogenesis [catalytic activity: fructose-1,6-bisphosphate + H2O = D-fructose-6-phosphate + phosphate] Fructose 1,6-bisphosphatase GlpX Rv1099c, (MTV017.52c), len: 362 aa. glpX, class II fructose 1,6-bisphosphatase (See Movahedzadeh et al., 2004), highly similar to P44811|GLPX_HAEIN GLPX protein homolog (believed to be involved in glycerol metabolism) (333 aa), FASTA scores: opt: 763, E():0, (46.2% identity in 327 aa overlap); and Q03224|YWJI_BACSU hypothetical protein from Bacillus subtilis (321aa), FASTA scores: opt: 1092, E(): 0, (52.1% identity in 313 aa overlap). Equivalent to AL049491|MLCB1222_10 Mycobacterium leprae (355 aa), (93.0% identity in 328 aa overlap). N-terminus extended since first submission (previously 328 aa). P9WN21 No intermediary metabolism and respiration O53447 P9WN21 GO:0006071 P9WN21 NC_000962.3 Mycobrowser_v5 CDS 1233966 1234223 . - 0 Rv1107c xseB Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides [catalytic activity: exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphomononucleotides.] Probable exodeoxyribonuclease VII (small subunit) XseB (exonuclease VII small subunit) Rv1107c, (MTV017.60c), len: 85 aa. Probable xseB, exonuclease VII small subunit (see citation below). Equivalent to AL049491|MLCB1222_6 Mycobacterium leprae (87 aa) (77.9% identity in 68 aa overlap). Similar to P43914|EX7S_HAEIN exodeoxyribonuclease small subunit from H. influenzae (84 aa), FASTA scores: opt: 126, E(): 0.006, (37.3% identity in 67 aa overlap); and P22938|EX7S_ECOLI exodeoxyribonuclease small subunit from Escherichia coli (79 aa), FASTA scores: opt: 125, E(): 0.0067, (39.7% identity in 58 aa overlap). Belongs to the XseB family. P9WF29 No information pathways P67456 P9WF29 GO:0005737,GO:0008855,GO:0009318,GO:0006308 3.1.11.6 P9WF29 NC_000962.3 Mycobrowser_v5 CDS 1234213 1235460 . - 0 Rv1108c xseA Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides [catalytic activity: exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphomononucleotides] Probable exodeoxyribonuclease VII (large subunit) XseA (exonuclease VII large subunit) Rv1108c, (MTV017.61c), len: 415 aa. Probable xseA, exodeoxyribonuclease VII large subunit (see Mizrahi & Andersen 1998). Equivalent to AL049491|MLCB1222_5 Mycobacterium leprae (428 aa) (81.5% identity in 411 aa overlap). Similar to many e.g. P04994|EX7L_ECOLI exodeoxyribonuclease large subunit from Escherichia coli (456 aa), FASTA scores: opt: 581, E(): 1.6 e-30, (30.8% identity in 425 aa overlap); also similar to the exodeoxyribonuclease in Bacillus subtilis, H. influenzae and H. pylori. Belongs to the XseA family. P9WF31 No information pathways P67447 P9WF31 GO:0005737,GO:0008855,GO:0009318,GO:0006308 3.1.11.6 P9WF31 NC_000962.3 Mycobrowser_v5 CDS 1237209 1238192 . - 0 Rv1111c Rv1111c Function unknown Conserved hypothetical protein Rv1111c, (MTV017.64c), len: 327 aa. Conserved hypothetical protein, N-terminal domain is hydrophobic, C-terminal half is very rich in Arg. Equivalent to AL049491|MLCB1222_2 hypothetical protein from Mycobacterium leprae (379 aa) (46.0% identity in 374 aa overlap). Start changed since first submission. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O86351 No conserved hypotheticals O86351 NC_000962.3 Mycobrowser_v5 CDS 1238255 1239328 . + 0 Rv1112 Rv1112 Unknown Probable GTP binding protein Rv1112, (MTCY22G8.01-MTV017.65), len: 357 aa. Probable GTP binding protein, similar to YCHF_HAEIN|P44681 probable gtp-binding protein (362 aa), FASTA scores: opt: 1189, E(): 0, (52.7% identity in 357 aa overlap). Equivalent to AL049491|MLCB1222_1 hypothetical protein from Mycobacterium leprae (356 aa) (85.9% identity in 354 aa overlap0. Contains PS00017 ATP/GTP-binding site motif A (P-loop). O53459 No conserved hypotheticals O53459 O53459 GO:0005622,GO:0005525 O53459 NC_000962.3 Mycobrowser_v5 CDS 1243707 1245107 . + 0 Rv1121 zwf1 Involved in pentose phosphate pathway (first step) [catalytic activity: D-glucose 6-phosphate + NADP(+) = D-glucono-1,5-lactone 6-phosphate + NADPH.] Probable glucose-6-phosphate 1-dehydrogenase Zwf1 (G6PD) Rv1121, (MTCY22G8.10), len: 466 aa. Probable zwf1, glucose-6-phosphate 1-dehydrogenase, highly similar to many e.g. G6PD_E COLI|P22992 Escherichia coli (491 aa), FASTA scores: opt: 642, E(): 0, (35.8% identity in 478 aa overlap). Mycobacterium tuberculosis has two genes for ZWF, this one is highly divergent. Belongs to the glucose-6-phosphate dehydrogenase family. Note that previously known as zwf. Nucleotide position 1244700 in the genome sequence has been corrected, T:C resulting in L332L. P9WN71 No intermediary metabolism and respiration P0A586 P9WN71 GO:0006006,GO:0004345,GO:0055114 1.1.1.49 P9WN71 NC_000962.3 Mycobrowser_v5 CDS 1245129 1246151 . + 0 Rv1122 gnd2 Involved in hexose monophosphate shunt (pentose phosphate pathway). [catalytic activity: 6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH] Probable 6-phosphogluconate dehydrogenase, decarboxylating Gnd2 Rv1122, (MTCY22G8.11), len: 340 aa. Probable gnd2, 6-phosphogluconate dehydrogenase, decarboxylating, highly similar to Q53917 6-phosphogluconate dehydrogenase from Streptomyces coelicolor (291 aa), fasta scores: opt: 431, E(): 2.2e-20, (44.5% identity in 335 aa overlap). Also similar to Rv1844c|MTCY359.29|gnd1 probable 6-phosphogluconate dehydrogenase from Mycobacterium tuberculosis (485 aa), FASTA score: (33.0% identity in 351 aa overlap). Note that Rv1844c|MTCY359.29|gnd1 is most similar to gnd's from Gram negative organisms, while gnd2 is most similar to gnd's from Gram positive organisms. Belongs to the 6-phosphogluconate dehydrogenase family. O06574 No intermediary metabolism and respiration O06574 O06574 GO:0055114,GO:0006098,GO:0004616,GO:0050661 1.1.1.44 O06574 NC_000962.3 Mycobrowser_v5 CDS 1246144 1247052 . - 0 Rv1123c bpoB Supposedly involved in detoxification reactions. Possible peroxidase BpoB (non-haem peroxidase) Rv1123c, (MTCY22G8.12c), len: 302 aa. Possible bpoB, peroxidase (non-haem peroxidase), with some similarity to a range of enzymes from several organisms including: DEH1_MORSP|Q01398 haloacetate dehalogenase from Moraxella sp. (294 aa), FASTA scores: opt: 201, E(): 2.1e-06, (35.8% identity in 134 aa overlap); and BPA1_STRAU|P33912 non-haem bromoperoxidase bpo-a1 from Streptomyces aureofaciens (274 aa), FASTA scores: opt: 187, E(): 1.6e-05, (23.1% identity in 281 aa overlap). Similar to several other Mycobacterium tuberculosis proteins, probable epoxide hydrolases and non-heme bromoperoxidases e.g. Rv1938, Rv3617, Rv3473c, Rv3171c, etc. Contains PS00216 Sugar transport proteins signature 1. O06575 No virulence, detoxification, adaptation O06575 O06575 GO:0004601,GO:0055114 1.11.1.- O06575 NC_000962.3 Mycobrowser_v5 CDS 2200938 2201249 . + 0 Rv1953 vapC14 Unknown Possible toxin VapC14 Rv1953, (MTCY09F9.11c), len: 103 aa. Possible vapC14, toxin, part of toxin-antitoxin (TA) operon with Rv1952, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Some similarity to O33827 plasmid stability-like protein from Thiobacillus ferrooxidans (143 aa), FASTA scores: opt: 170, E(): 3.5e-06, (45.3% identity in 75 aa overlap). P9WF99 No virulence, detoxification, adaptation P95261 P9WF99 NC_000962.3 Mycobrowser_v5 CDS 1248082 1249326 . + 0 Rv1125 Rv1125 Function unknown Conserved hypothetical protein Rv1125, (MTCY22G8.14), len: 414 aa. Conserved hypothetical protein. Similar to AL133278|SCM11.13 hypothetical protein from Streptomyces coelicolor (446 aa), FASTA scores: opt: 182, E(): 0.0005, (28.1% identity in 437 aa overlap). O06577 No conserved hypotheticals O06577 NC_000962.3 Mycobrowser_v5 CDS 1249330 1249935 . - 0 Rv1126c Rv1126c Function unknown Conserved protein Rv1126c, (MTCY22G8.15c), len: 201 aa. Conserved protein, similar in N-terminus to O05567|MLCB33.17 hypothetical protein from Mycobacterium leprae (141 aa), FASTA scores: opt: 332, E(): 1.4e-23, (58.4% identity in 101 aa overlap). O06578 No conserved hypotheticals O06578 O06578 NC_000962.3 Mycobrowser_v5 CDS 1251617 1252972 . - 0 Rv1128c Rv1128c Unknown Conserved hypothetical protein Rv1128c, (MTCY22G8.17c), len: 451 aa. Conserved hypothetical protein, in REP13E12 degenerate repeat, highly similar to several Mycobacterium tuberculosis proteins in REP13E12 repeats e.g. Rv1148c, Rv1945, Rv3467, etc. P9WM57 No insertion seqs and phages O06580 P9WM57 P9WM57 NC_000962.3 Mycobrowser_v5 CDS 1254555 1256135 . + 0 Rv1130 prpD Involved in methyl citrate cycle Possible methylcitrate dehydratase PrpD Rv1130, (MTCY22G8.19), len: 526 aa. Possible prpD, methylcitrate dehydratase (MCD), some similarity to AP000063|AP000063_192 hypothetical protein from Aeropyrum pernix (479 aa), FASTA scores: opt: 717, E(): 0, (34.3% identity in 443 a a overlap), and to PRPD_ECOLI|P77243 prpd protein from Escherichia coli (483aa), FASTA scores: opt: 234, E(): 3.3e-08, (27.0% identity in 429 aa overlap). Predicted possible vaccine candidate (See Zvi et al., 2008). O06582 No intermediary metabolism and respiration O06582 O06582 GO:0019543,GO:0047547 O06582 NC_000962.3 Mycobrowser_v5 CDS 1264314 1264556 . + 0 Rv1135A Rv1135A Function unknown, but involved in lipid degradation [catalytic activity: 2 acetyl-CoA = CoA + acetoacetyl-CoA]. Possible acetyl-CoA acetyltransferase (acetoacetyl-CoA thiolase) Rv1135A, len: 80 aa. Possible acetyl-CoA acetyltransferase (possible gene fragment), highly similar to other acetyl-CoA acetyltransferases e.g. C-terminal part of Rv3556c|Z92774|MTCY6G11_2|MTCY06G11.03|fadA6 acetyl-CoA acetyltransferase from Mycobacterium tuberculosis (386 aa), FASTA scores: opt: 219, E(): 5.7e-09, (63.6% identity in 55 aa overlap). L7N682 No lipid metabolism Q8VK64 L7N682 GO:0008152,GO:0003985 2.3.1.9 L7N682 NC_000962.3 Mycobrowser_v5 CDS 1266485 1266985 . - 0 Rv1139c Rv1139c Function unknown Conserved hypothetical membrane protein Rv1139c, (MTCI65.06c), len: 166 aa. Conserved hypothetical membrane protein. Highly similar to P54158|YBPQ_BACSU hypothetical Bacillus subtilis protein, YBPQ (168 aa), FASTA scores: opt: 446, E(): 2.2e-26, (38.4% identity in 164 aa overlap). Some similarity to Mycobacterium tuberculosis hypothetical proteins, Rv0740, Rv0750. O06539 No cell wall and cell processes O06539 O06539 GO:0016021,GO:0004671,GO:0006481 O06539 NC_000962.3 Mycobrowser_v5 CDS 1257325 1259055 . + 0 Rv1132 Rv1132 Unknown Conserved membrane protein Rv1132, (MTCY22G8.21), len: 576 aa. Conserved membrane protein, similar to O06827|Rv1431|MTCY493.23C membrane protein from Mycobacterium tuberculosis (589 aa), fasta scores: opt: 1811, E(): 0, (48.2% identity in 585 aa overlap). O06583 No cell wall and cell processes O06583 O06583 O06583 NC_000962.3 Mycobrowser_v5 CDS 1262272 1264128 . - 0 Rv1135c PPE16 Function unknown PPE family protein PPE16 Rv1135c, (MTCI65.02c), len: 618 aa. PPE16, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins. Similar to Rv2356c (59.6% identity in 627 aa overlap); etc.. Predicted to be an outer membrane protein (See Song et al., 2008). P9WI29 No PE/PPE Q79FS0 P9WI29 P9WI29 NC_000962.3 Mycobrowser_v5 CDS 1267347 1268195 . + 0 Rv1140 Rv1140 Function unknown Probable integral membrane protein Rv1140, (MTCI65.07), len: 282 aa. Probable integral membrane protein. Weak similarity in C-terminus to hypothetical Escherichia coli proteins YPRA and YPRB, possibly membrane-bound e.g. YPRA_ECOLI hypothetical 24.3 kDa protein (URF 1) (217 aa), FASTA scores: opt: 166, E(): 0.00062, (31.0% identity in 158 aa overlap). O06540 No cell wall and cell processes O06540 O06540 GO:0016020 NC_000962.3 Mycobrowser_v5 CDS 1268203 1269009 . - 0 Rv1141c echA11 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Probable enoyl-CoA hydratase EchA11 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv1141c, (MTCI65.08c), len: 268 aa. Probable echA11, enoyl-CoA hydratase, similar to others e.g. P24162|ECHH_RHOCA probable enoyl-CoA hydratase from Rhodobacter capsulatus(257 aa); CAA66096.1|X97452 enoyl-CoA isomerase from Escherichia coli (262 aa), FASTA scores: opt: 513, E():1e-25, (36.1% identity in 249 aa overlap); etc. Also similarity with naphthoate synthases. Also highly similar to downstream ORF Rv1142c|MTCI65.09|echA10 probable enoyl-CoA hydratase from Mycobacterium tuberculosis (268 aa), FASTA scores: opt: 1225, E(): 0, (72.3% identity in 267 aa overlap). O06541 No lipid metabolism O06541 O06541 GO:0016853,GO:0008152,GO:0004300 4.2.1.17 O06541 NC_000962.3 Mycobrowser_v5 CDS 1270062 1271144 . + 0 Rv1143 mcr Required for bile acid synthesis and for catabolism of branched-chain fatty acids Probable alpha-methylacyl-CoA racemase Mcr (2-methylacyl-CoA racemase) (2-arylpropionyl-CoA epimerase ) Rv1143, (MTCI65.10), len: 360 aa. Probable mcr, alpha-methylacyl-CoA racemase. Strong similarity to other alpha-methylacyl-CoA racemases and also some similarity to L-carnitine dehydratase e.g. U89905|g1552373 methylacyl-CoA racemase alpha from Norway rat (361 aa), FASTA scores: opt: 1035, E():0, (47.2% identity in 339 aa overlap). Equivalent to (but longer than) Z94723|MLCB33_13 Mycobacterium leprae (253 aa) (85.3% identity in 245 aa overlap). Also similar to Mycobacterium tuberculosis putative racemases Rv0855, Rv1866, Rv3272. O06543 No lipid metabolism 1X74,2GCE,2GCI,2GD0,2GD2,2GD6 O06543 O06543 GO:0008152,GO:0008111 5.1.99.4 O06543 NC_000962.3 Mycobrowser_v5 CDS 1271156 1271908 . + 0 Rv1144 Rv1144 Function unknown; supposedly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv1144, (MTCI65.11), len: 250 aa. Probable short-chain dehydrogenase/reductase, highly similar to various dehydrogenases e.g. NP_104056.1|NC_002678 3-hydroxyacyl-CoA dehydrogenase type II from Mesorhizobium loti (253 aa); NP_251244.1|NC_002516 probable short-chain dehydrogenase from Pseudomonas aeruginosa (255 aa); AAK15008.1|AF233685_1|AF233685 short chain L-3-hydroxyacyl-CoA dehydrogenase from Mus musculus (261 aa); HSU73514|g1778354|XH98G2 human short-chain alcohol dehydrogenase from Homo sapiens (261 aa), FASTA scores: opt: 875, E(): 0, (60.1% identity in 253 aa overlap); etc. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. P9WGQ7 No intermediary metabolism and respiration O06544 P9WGQ7 GO:0055114,GO:0016491 1.-.-.- P9WGQ7 NC_000962.3 Mycobrowser_v5 CDS 1272423 1273334 . + 0 Rv1145 mmpL13a Unknown. Thought to be involved in fatty acid transport. Probable conserved transmembrane transport protein MmpL13a Rv1145, (MTCI65.12), len: 303 aa. Probable mmpL13a, conserved transmembrane transport protein (see citation below), member of RND superfamily, showing some similarity to putative Mycobacterial and Streptomyces membrane proteins e.g. MTCY987|g1781238 from Mycobacterium tuberculosis (962 aa), FASTA scores: opt: 213, E(): 1.9e-06, (28.0% identity in 296 aa overlap); etc. Strong similarity to U92075|MMU92075_5 hypothetical protein from Mycobacterium marinum (256 aa), FASTA scores: opt: 957, E(): 0, (57.6% identity in 257 aa overlap). Should continue as mmpL13B|Rv1146, but frameshift required. Sequence has been checked and is identical in M. tuberculosis strain CDC1551, and Mycobacterium bovis strain AF2122/97. Belongs to the MmpL family. O06545 No cell wall and cell processes O06545 O06545 GO:0016021 O06545 NC_000962.3 Mycobrowser_v5 CDS 1278904 1279617 . - 0 Rv1151c Rv1151c Involved in transcriptional mechanism Transcriptional regulatory protein Rv1151c, (MTCI65.18c), len: 237 aa. Transcriptional regulatory protein, similar to others AE000776|AE000776_10 Aquifex aeolicus (239 aa), FASTA scores: opt: 725, E(): 0, (46.4% identity in 237 aa overlap); ECAE0002125|g1787358 Escherichia coli (279 aa), FASTA scores: opt: 464, E(): 1.3e-23, (36.7% identity in 240 aa overlap). P9WGG3 No regulatory proteins P66813 P9WGG3 GO:0006342,GO:0005737,GO:0016811,GO:0006476,GO:0008270,GO:0070403 3.5.1.- P9WGG3 NC_000962.3 Mycobrowser_v5 CDS 1279655 1280020 . + 0 Rv1152 Rv1152 Involved in transcriptional mechanism Probable transcriptional regulatory protein Rv1152, (MTCI65.19), len: 121 aa (Start uncertain). Probable transcriptional regulatory protein, some similarity to others e.g. YHCF_BACSU hypothetical transcriptional regulator (121 aa), FASTA scores: opt: 187, E(): 1.9e-06, (34.9% identity in 106 aa overlap). Helix turn helix motif from aa 42-63 (+3.10 SD). O06550 No regulatory proteins O06550 O06550 GO:0006355,GO:0006350,GO:0003700,GO:0005622 O06550 NC_000962.3 Mycobrowser_v5 CDS 1332980 1333858 . + 0 Rv1190 Rv1190 Function unknown Conserved hypothetical protein Rv1190, (MTCI364.02), len: 292 aa. Conserved hypothetical protein, similar to Rv1833c|Y0DA_MYCTU|Q50600 hypothetical 32.2 kDa protein cy1a11.10 (286 aa), fasta scores: opt: 331, E(): 1.4e-15, (29.0% identity in 272 aa overlap), also YU14_MYCTU|Q50670 putative haloalkane dehalogenase (300 aa), FASTA scores: opt: 239, E(): 2.2e-09, (29.9% identity in 298 aa overlap). O86348 No conserved hypotheticals O86348 O86348 GO:0016787 O86348 NC_000962.3 Mycobrowser_v5 CDS 1388685 1388978 . - 0 Rv1246c relE Function unknown Toxin RelE Rv1246c, (MTV006.18c), len: 97 aa. RelE, toxin, part of toxin-antitoxin (TA) operon with Rv1247c (See Pandey and Gerdes, 2005), highly similar to Rv2866|MTV003.12 hypothetical Mycobacterium tuberculosis protein (87 aa), FASTA scores: opt: 290, E(): 3.9e-24, (54.1% identity in 85 aa overlap). O50461 No virulence, detoxification, adaptation O50461 O50461 O50461 NC_000962.3 Mycobrowser_v5 CDS 1278269 1278820 . + 0 Rv1150 Rv1150 Possibly required for the transposition of an insertion element Possible transposase (fragment) Rv1150, (MTCI65.17), len: 183 aa. Possible fragment of transposase (pseudogene). Identical to C-terminal part of S21394 transposase of putative Mycobacterium tuberculosis is element (308 aa), FASTA scores: opt: 959, E(): 0, (99.3% identity in 145 aa overlap). The transposase described here may be made by a -1 frame shifting mechanism during translation that fuses Rv1149|MTCI65.16 and Rv1150|MTCI65.17. No evidence found to account for discrepancy with previously published sequence. Second copy is Rv1041c|MTCY10G2.08. Yes insertion seqs and phages NC_000962.3 Mycobrowser_v5 ncRNA 1283693 1283815 . + 0 MTB000072 mcr10 Unknown Putative small regulatory RNA mcr10, putative small regulatory RNA (See DiChiara et al., 2010). 5'-end mapped by 5'RLM-RACE in M. bovis BGC Pasteur, 3'-end not mapped, ~118 nt band detected by Northern blot. No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 1274900 1275550 . + 0 Rv1147 Rv1147 Function unknown Conserved protein Rv1147, (MTCI65.14), len: 216 aa. Conserved protein, similar to many conserved hypothetical proteins, and some similarity to several methyltransferases e.g. Q05197|PMTA_RHOSH phosphatidylethanolamine N-methyltransferase from R. sphaeroides (203 aa), FASTA scores: opt: 156, E(): 0.00073, (27.6% identity in 156 aa overlap). O06547 No conserved hypotheticals O06547 O06547 GO:0008168,GO:0008152 O06547 NC_000962.3 Mycobrowser_v5 CDS 1276300 1277748 . - 0 Rv1148c Rv1148c Unknown Conserved hypothetical protein Rv1148c, (MTCI65.15c), len: 482 aa. Conserved hypothetical ORF in REP13E12 degenerate repeat, nearly identical to other hypothetical Mycobacterium tuberculosis proteins in REP13E12 repeats, although similarity extends upstream past proposed f-Met start. Very similar to other REP13E12 proteins e.g. Rv1945, Rv3467, Rv0094c, Rv1128c etc. P9WM55 No insertion seqs and phages P0A5D9 P9WM55 P9WM55 NC_000962.3 Mycobrowser_v5 CDS 1277893 1278300 . + 0 Rv1149 Rv1149 Possibly required for the transposition of an insertion element Possible transposase Rv1149, (MTCI65.16), len: 135 aa. Possible transposase. Identical to 117 aa N-terminal region of S21394|X65618 transposase of Mycobacterium tuberculosis (308 aa), FASTA scores: opt: 823, E(): 0, (99.1% identity in 117 aa overlap). Second copy is Rv1042c|MTCY10G2.07. L0T897 No insertion seqs and phages L0T897 NC_000962.3 Mycobrowser_v5 CDS 1281429 1281872 . + 0 Rv1155 Rv1155 May be involved in biosynthesis of pyridoxine (vitamin B6) and pyridoxal phosphate. Oxidize PNP and PMP into pyridoxal 5'-phosphate (PLP) [catalytic activity: pyridoxamine 5'-phosphate + H2O + O2 = pyridoxal 5'-phosphate + NH3 + H2O2] Possible pyridoxamine 5'-phosphate oxidase (PNP/PMP oxidase) (pyridoxinephosphate oxidase) (PNPOX) (pyridoxine 5'-phosphate oxidase) Rv1155, (MTCI65.22), len: 147 aa. Possible pyridoxine 5'-phosphate oxidase (PNPOx) (See Biswal et al., 2005; Canaan et al., 2005). Similar to hypothetical proteins e.g. AL079356|SC6G9.20 Streptomyces coelicolor (144 aa), FASTA scores: opt: 478, E(): 2.8e-26, (55.7% identity in 140 aa overlap); and Mycobacterium tuberculosis proteins Rv1875, Rv0121c, Rv2074. O06553 No intermediary metabolism and respiration 1W9A,1XXO,1Y30,2AQ6 O06553 O06553 GO:0010181 O06553 NC_000962.3 Mycobrowser_v5 CDS 1283056 1284171 . - 0 Rv1157c Rv1157c Function unknown Conserved ala-, pro-rich protein Rv1157c, (MTCI65.24c), len: 371 aa. Conserved Ala-, Pro-rich protein, similar to other proline rich proteins and extensins e.g. GBU04267|g451543 sea-island cotton proline-rich protein of cotton fiber (214 aa), FASTA scores: opt: 305, E(): 3.9e-05, (35.7% identity in 182 aa overlap). Has hydrophobic stretch at N-terminus suggestive of secretion signal. First start taken. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06555 No conserved hypotheticals O06555 GO:0005199 NC_000962.3 Mycobrowser_v5 CDS 1701295 1702593 . + 0 Rv1510 Rv1510 Unknown Conserved probable membrane protein Rv1510, (MTCY277.32), len: 432 aa. Probable membrane protein. Highly similar to Rv3630|MTCY15C10.22 (431 aa), FASTA scores: E(): 0, (70.8% identity in 424 aa overlap). P9WLW1 No cell wall and cell processes P9WLW1 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1286284 1286568 . - 0 Rv1159A Rv1159A Unknown Unknown protein Rv1159A, len: 94 aa. Unknown protein. P0A5S2 No conserved hypotheticals P0A5S2 P0A5S2 GO:0006729,GO:0008124 4.2.1.96 NC_000962.3 Mycobrowser_v5 CDS 1299822 1300124 . - 0 Rv1169c lipX Function unknown PE family protein. Possible lipase LipX. Rv1169c, (MTV005.05c), len: 100 aa. Possible lipX, lipase. Member of the Mycobacterium tuberculosis PE family of proteins (see Brennan & Delogu 2002), e.g. O05297|Z93777|MTCI364.07 (99 aa), FASTA scores: opt: 209, E(): 1.6e-15, (37.4% identity in 99 aa overlap). Also simlar to the N-terminus of P77909|U76006 esterase/lipase from Mycobacterium tuberculosis (437 aa), FASTA scores: opt: 193, E(): 4.4e-14, (37.2% identity in 94 aa overlap). Contains a helix-turn-helix motif from aa 88-109 (+2.76 SD). Predicted possible vaccine candidate (See Zvi et al., 2008). Q79FR5 No PE/PPE Q79FR5 Q79FR5 Q79FR5 NC_000962.3 Mycobrowser_v5 CDS 1286595 1287020 . + 0 Rv1160 mutT2 Involved in the go system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite DA and DC residues of template DNA with almost equal efficiency thus leading to A.T to G.C transversions. MUTT2 specifically degrades 8-oxo-dGTP to the monophosphate. Probable mutator protein MutT2 (7,8-dihydro-8-oxoguanine-triphosphatase) (8-oxo-dGTPase) Rv1160, (MTCI65.27), len: 141 aa. Probable mutT2, mutator protein or homolog (see citation below). More similar to D908197|g1742860 MutT homolog from Escherichia coli (135 aa), FASTA scores: opt: 226, E():1.1e-08, (39.7% identity in 116 aa overlap); than to MUTT_ECOLI|P08337 mutator mutt protein from Escherichia coli (129 aa), FASTA scores: opt: 180, E(): 1.2e-05, (27.1% identity in 129 aa overlap). Contains PS00893 mutT domain signature. P9WIY1 No information pathways O06558 P9WIY1 GO:0006260,GO:0016787,GO:0046872,GO:0006281 3.6.1.- P9WIY1 NC_000962.3 Mycobrowser_v5 CDS 1287328 1291026 . + 0 Rv1161 narG Nitrate reduction [catalytic activity: nitrite + acceptor = nitrate + reduced acceptor]. Respiratory nitrate reductase (alpha chain) NarG Rv1161, (MTCI65.28), len: 1232 aa. narG, respiratory nitrate reductase alpha chain. Similar to others e.g. NARG_BACSU nitratereductase alpha chain from Bacillus subtilis (1228 aa), FASTA scores: opt: 4218, E(): 0, (50.3% identity in 1229 aa overlap); etc. Also highly similar to N-terminal part of Rv1736c|MTCY04C12.21c|NARX probable nitrate reductase from Mycobacterium tuberculosis (85.1% identity in 281 aa overlap). Contains prokaryotic molybdopterin oxidoreductase signatures 1 and 2 (PS00551, PS00490). Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. P9WJQ3 No intermediary metabolism and respiration O06559 P9WJQ3 GO:0009055,GO:0022900,GO:0030151,GO:0042128,GO:0008940,GO:0009325,GO:0005886,GO:0006810,GO:0051539 1.7.99.4 P9WJQ3 NC_000962.3 Mycobrowser_v5 CDS 1292798 1293403 . + 0 Rv1163 narJ Nitrate reduction [catalytic activity: nitrite + acceptor = nitrate + reduced acceptor]. Probable respiratory nitrate reductase (delta chain) NarJ Rv1163, (MTCI65.30), len: 201 aa. Probable narJ, respiratory nitrate reductase delta chain. Similar to others e.g. P42178|NARJ_BACSU nitrate reductase delta chain from Bacillus subtilis (184 aa), FASTA scores: opt: 254, E(): 1.9e-10, (31.8% identity in 179 aa overlap); etc. Strong similarity to region from aa 260 - 410 of Rv1736c|MTCY04C12.21c|NARX probable nitrate reductase from Mycobacterium tuberculosis (64.8% identity in 159 aa overlap). O06561 No intermediary metabolism and respiration O06561 O06561 GO:0009325,GO:0055114,GO:0008940 1.7.99.4 O06561 NC_000962.3 Mycobrowser_v5 CDS 1353157 1353525 . + 0 Rv1209 Rv1209 Function unknown Conserved protein Rv1209, (MTCI364.21), len: 122 aa. Conserved protein, containing a hydrophobic N-terminus. Similar to Q49956|U1756M hypothetical protein from Mycobacterium leprae (114 aa), FASTA scores: opt: 524, E(): 8.9e-29, (78.6% identity in 112 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). O05310 No conserved hypotheticals O05310 NC_000962.3 Mycobrowser_v5 CDS 1676941 1677375 . + 0 Rv1487 Rv1487 Unknown Conserved membrane protein Rv1487, (MTCY277.08), len: 144 aa. Conserved membrane protein. Highly similar to O07404|AF002133 MAV145 from Mycobacterium avium (145 aa), FASTA scores: opt: 667, E(): 0, (72.5% identity in 142 aa overlap). Also similar to AL079332|SCI5.05 hypothetical protein from Streptomyces coelicolor (143 aa), FASTA scores: opt: 344, E(): 1.3e-15, (44.8% identity in 134 aa overlap). P71767 No cell wall and cell processes P71767 P71767 P71767 NC_000962.3 Mycobrowser_v5 CDS 1296152 1298059 . + 0 Rv1166 lpqW Unknown Probable conserved lipoprotein LpqW Rv1166, (MTV005.02), len: 635 aa. Probable lpqW, conserved lipoprotein, almost identical in part to G2384665|AF009358 Mycobacterium tuberculosis gene fragment ORFA2-898 (fragment) (59 aa) (93.9% identity in 49 aa overlap) (see * below). Also similar to Rv1280c and Rv2585c. Contains possible N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. [* Note: Unpublished. Identification of Mycobacterium tuberculosis peptides that stimulate immune human peripheral blood monocytes. Nano F.E., Doran J.L., Treit J.D., Moran A.J.] P9WGU7 No cell wall and cell processes O50422 P9WGU7 GO:0008654,GO:0006810,GO:0005215,GO:0009405 P9WGU7 NC_000962.3 Mycobrowser_v5 CDS 1298087 1298692 . - 0 Rv1167c Rv1167c Involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv1167c, (MTV005.03c), len: 201 aa. Probable transcriptional regulator, similar to several e.g. D1022772|D85417 hemR from Propionibacterium freudenreichii (243 aa), FASTA scores: opt: 268, E(): 5.4e-16, (35.9% identity in 198 aa overlap) and AL022268|SC4H2.32 Streptomyces coelicolor (111 aa), FASTA scores: opt: 274, E(): 5e-11, (55.1% identity in 89 aa overlap). O50423 No regulatory proteins O50423 O50423 GO:0006350,GO:0003700,GO:0006355 O50423 NC_000962.3 Mycobrowser_v5 CDS 1298764 1299804 . - 0 Rv1168c PPE17 Function unknown PPE family protein PPE17 Rv1168c, (MTV005.04c), len: 346 aa. PPE17, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, similar to many e.g. E332789|Z98268|MTCI125.27C (385 aa), FASTA scores: opt: 504, E(): 0, (36.6% identity in 388 aa overlap). P9WI27 No PE/PPE Q7D8Q2 P9WI27 P9WI27 NC_000962.3 Mycobrowser_v5 CDS 1334927 1335754 . + 0 Rv1192 Rv1192 Unknown Unknown protein Rv1192, (MTCI364.04), len: 275 aa. Unknown protein, contains PS00120 lipases, serine active site. O05294 No conserved hypotheticals O05294 O05294 O05294 NC_000962.3 Mycobrowser_v5 CDS 1357759 1359444 . - 0 Rv1215c Rv1215c Function unknown Conserved protein Rv1215c, (MTCI364.27c), len: 561 aa. Conserved protein, low similarity to Rv1835c|Y0D8_MYCTU|Q50598 hypothetical 69.9 kDa protein cy1a11.08 (628 aa), FASTA scores: opt: 257, E(): 1.3e-09, (34.1% identity in 185 aa overlap). O05316 No conserved hypotheticals O05316 O05316 GO:0008152,GO:0008239 O05316 NC_000962.3 Mycobrowser_v5 CDS 1301755 1302681 . - 0 Rv1172c PE12 Function unknown PE family protein PE12 Rv1172c, (MTV005.08c), len: 308 aa. PE12, Member of the Mycobacterium tuberculosis PE family of proteins (see Brennan & Delogu 2002), e.g. P71748|Z81368|MTCY253.25C (361 aa), FASTA scores: opt: 483, E(): 7.8e-22, (46.4% identity in 192 aa overlap). L7N693 No PE/PPE Q7D8P8 L7N693 L7N693 NC_000962.3 Mycobrowser_v5 CDS 1305669 1306001 . - 0 Rv1174c TB8.4 Unknown function (secreted protein) Low molecular weight T-cell antigen TB8.4 Rv1174c, (MTV005.10c), len: 110 aa. TB8.4, low molecular weight T-cell antigen (see citations below), hypothetical unknown secreted protein. Predicted to be an outer membrane protein (See Song et al., 2008). Predicted possible vaccine candidate (See Zvi et al., 2008). O50430 No cell wall and cell processes O50430 NC_000962.3 Mycobrowser_v5 CDS 1306202 1308226 . - 0 Rv1175c fadH Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-2- enoyl-CoA [catalytic activity: trans-2,3-didehydroacyl-CoA + NADP(+) = trans,trans-2,3,4,5-tetradehydroacyl-CoA + NADPH]. Probable NADPH dependent 2,4-dienoyl-CoA reductase FadH (2,4-dienoyl coenzyme A reductase) (4-enoyl-CoA reductase) Rv1175c, (MTV005.11c), len: 674 aa. Probable fadH, NADPH-dependent 2,4-dienoyl-CoA reductase, highly similar to others e.g. NP_251782.1|NC_002516 2,4-dienoyl-CoA reductase FadH1 from Pseudomonas aeruginosa (679 aa); CAC01564.1|AL391039 2,4-dienoyl-CoA reductase [NADPH] from Streptomyces coelicolor (671 aa); P42593|FADH_ECOLI 2,4-dienoyl-CoA reductase from Escherichia coli (671 aa), FASTA scores: opt: 2344, E(): 0, (53.1% identity in 671 aa overlap); etc. Also similar to Rv3359|MTV004.16 putative oxidoreductase from Mycobacterium tuberculosis (396 aa). O50431 No lipid metabolism O50431 O50431 GO:0050660,GO:0010181,GO:0055114,GO:0008670 1.3.1.34 O50431 NC_000962.3 Mycobrowser_v5 CDS 1365344 1365808 . + 0 Rv1222 rseA Regulates negatively SIGE|Rv1221 Anti-sigma factor RseA Rv1222, (MTCI61.05), len: 154 aa. RseA, anti-sigma factor (See Dona et al., 2008). Identical to O06290|MTU87242 (but shorter due to different start site chosen by proximity of RBS). Equivalent to O05736|U87308|MAU87308_2 hypothetical protein from Mycobacterium avium (133 aa), FASTA scores: opt: 644, E(): 7e-32, (86.2% identity in 109 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). L0T905 No information pathways L0T905 NC_000962.3 Mycobrowser_v5 CDS 1309005 1309331 . + 0 Rv1177 fdxC Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Probable ferredoxin FdxC Rv1177, (MTV005.13), len: 108 aa. Probable fdxC, ferredoxin, equivalent to NP_302047.1|NC_002677 ferredoxin from Mycobacterium leprae (108 aa); P00215|FER_MYCSM ferredoxin from Mycobacterium smegmatis (106 aa), FASTA scores: opt: 705, E(): 0, (87.7% identity in 106 aa overlap). Also highly similar to many e.g. JH0239 ferredoxin precursor from Saccharopolyspora erythraea (105 aa); P24496|FER_SACER ferredoxin from Saccharopolyspora erythraea (106 aa); etc. Contains PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature. Belongs to the bacterial type ferredoxin family. Cofactor: binds 1 4FE-4S cluster and a 3FE-4S cluster (by similarity). O50433 No intermediary metabolism and respiration O50433 O50433 GO:0009055,GO:0046872,GO:0055114,GO:0016491,GO:0051539 1.-.-.- O50433 NC_000962.3 Mycobrowser_v5 CDS 1313725 1315191 . + 0 Rv1180 pks3 Potentially involved in some intermediate steps for the synthesis of a polyketide molecule which may be involved in secondary metabolism. Supposedly involved in stationary-phase survival. Probable polyketide beta-ketoacyl synthase Pks3 Rv1180, (MTV005.16), len: 488 aa. Probable polyketide beta-ketoacyl synthase, equivalent to a predicted homologous protein from Mycobacterium smegmatis (see citation below), and similar to the N-terminus of many polyketide synthases e.g. MCAS_MYCBO|Q02251 mycocerosic acid synthase from Mycobacterium bovis (2110 aa), FASTA scores: opt: 2115, E(): 0, (66.5% identity in 472 aa overlap). Also similar to, and same length as P96284|Z83858|MTCY24G1.02 M. tuberculosis (496 aa), FASTA scores: opt: 1424, E(): 0, (50.9% identity in 444 aa overlap). Contains possible signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site, also PS00606 Beta-ketoacyl synthases active site. Belongs to the beta-ketoacyl-ACP synthases family. Alternative nucleotide at position 1315191 (a->C; Stop489Y) has been observed. Rv1180/Rv1181 fusion has been called msl3. O50436 No lipid metabolism O50436 O50436 GO:0009058,GO:0008415 2.3.1.- A0A089QRB9 NC_000962.3 Mycobrowser_v5 CDS 1315234 1319982 . + 0 Rv1181 pks4 Potentially involved in some intermediate steps for the synthesis of a polyketide molecule which may be involved in secondary metabolism. Probable polyketide beta-ketoacyl synthase Pks4 Rv1181, (MTV005.17), len: 1582 aa. Probable pks4, polyketide synthase, similar to many e.g. MCAS_MYCBO|Q02251 mycocerosic acid synthase from Mycobacterium bovis (2110 aa), FASTA scores: opt: 3518, E(): 0, (59.7% identity in 1614 aa overlap). Note that this similarity extends upstream of the first initiation codon into the upstream MTV005.16; the stop codon at the end of MTV005.16 is present in at least 4 independent clones (BAC, cosmid and pUC) from the genome (however an alternative nucleotide at position 1315191 (a->C; Stop489Y) has also been observed). The two CDS's may represent separate modules of the polyketide synthase. Rv1180/Rv1181 fusion has been called msl3. O50437 No lipid metabolism O50437 O50437 GO:0008415,GO:0009058,GO:0048037,GO:0055114,GO:0016491,GO:0008270,GO:0000036 2.3.1.- A0A089QRB9 NC_000962.3 Mycobrowser_v5 CDS 1320035 1321453 . + 0 Rv1182 papA3 Function unknown; thought to be involved in lipid metabolism. Probable conserved polyketide synthase associated protein PapA3 Rv1182, (MTV005.18), len: 472 aa. Probable papA3, conserved polyketide synthase (PKS) associated protein, similar to other Mycobacterial hypothetical proteins e.g. Q49618|U00010 B1170_C1_180 from Mycobacterium leprae (471 aa), FASTA scores: opt: 2526, E(): 0, (75.6% identity in 471 aa overlap). Similar to other Mycobacterium tuberculosis hypothetical papA proteins; Rv3824c, Rv3820c, Rv1528c. P9WIK5 No lipid metabolism O50438 P9WIK5 P9WIK5 NC_000962.3 Mycobrowser_v5 CDS 1332092 1332964 . + 0 Rv1189 sigI The sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released Possible alternative RNA polymerase sigma factor SigI Rv1189, (MTV005.25-MTCI364.01), len: 290 aa. Possible sigI, alternative RNA polymerase sigma factor (see Gomez et al., 1997; Chen et al., 2000), similar to several e.g. O05767|U87307 extracytoplasmic function alternative sigma factor (sigE) from Mycobacterium smegmatis (204 aa), FASTA scores: opt: 239, E(): 1.3e-09, (32.9% identity in 167 aa overlap). P9WGH3 No information pathways O50445 P9WGH3 GO:0016987,GO:0003700,GO:0006352,GO:0006355 P9WGH3 NC_000962.3 Mycobrowser_v5 CDS 1340659 1340955 . + 0 Rv1197 esxK Function unknown ESAT-6 like protein EsxK (ESAT-6 like protein 3) Rv1197, (MT1235, MTCI364.09), len: 98 aa. EsxK, ESAT-6 like protein (see citation below). Member of M. tuberculosis hypothetical QILSS protein family with Rv1038c, etc. Almost identical to MTCY98.023c (98 aa) (99.0% identity in 98 aa overlap) and MTCY10G2.11 (98 aa), FASTA scores: opt: 643, E(): 0, (99.0% identity in 98 aa overlap); highly similar to Q49945|U1756C from Mycobacterium leprae (100 aa), FASTA scores: opt: 377, E(): 8e-21, (58.3% identity in 96 aa overlap). Belongs to the ESAT6 family. P9WNJ7 No cell wall and cell processes P9WNJ7 P9WNJ7 NC_000962.3 Mycobrowser_v5 CDS 1324532 1325611 . - 0 Rv1184c Rv1184c Function unknown Possible exported protein Rv1184c, (MTV005.20c), len: 359 aa. Possible exported protein with potential N-terminal signal sequence. Similar to several Mycobacterial hypothetical proteins e.g. Q49633|U00010 Protein B1170_F3_112 from M. leprae (391 aa), FASTA scores: opt: 1422, E(): 0, (62.7% identity in 338 aa overlap). Also similar to Rv3822, Rv3539, Rv1430, Rv0151c, etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O50440 No cell wall and cell processes O50440 O50440 NC_000962.3 Mycobrowser_v5 CDS 1325776 1327512 . - 0 Rv1185c fadD21 Function unknown, but supposedly involved in lipid degradation. Probable fatty-acid-AMP ligase FadD21 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) Rv1185c, (MTV005.21c), len: 578 aa. Probable fadD21, fatty-acid-AMP synthetase, highly similar to several from Mycobacteria e.g. NP_301895.1|NC_002677 possible acyl-CoA synthase from Mycobacterium leprae (579 aa); P71495|U75685 acyl-CoA synthase from Mycobacterium bovis (582 aa), FASTA scores: opt: 2388, E(): 0, (61.8% identity in 579 aa overlap); etc. Seems to belong to the ATP-dependent AMP-binding enzyme family. Nucleotide position 1327402 in the genome sequence has been corrected, T:C resulting in E37E. P9WQ49 No lipid metabolism P63523 P9WQ49 GO:0016874,GO:0006631 6.2.1.- P9WQ49 NC_000962.3 Mycobrowser_v5 CDS 1327689 1329305 . - 0 Rv1186c Rv1186c Function unknown Conserved protein Rv1186c, (MTV005.22c), len: 538 aa. Conserved protein, similar to AL117385|SC5G9.24 hypothetical protein from Streptomyces coelicolor (555 aa), FASTA scores: opt: 485, E(): 2.3e-23, (32.6% identity in 568 aa overlap). Contains helix turn helix motif from aa 488-509 (+2.81 SD). O50442 No conserved hypotheticals O50442 O50442 NC_000962.3 Mycobrowser_v5 CDS 1339349 1340524 . + 0 Rv1196 PPE18 Function unknown PPE family protein PPE18 Rv1196, (MTCI364.08), len: 391 aa. PPE18 (alternate gene name: mtb39a). Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, highly similar to others e.g. Y07P_MYCTU|Q11031 hypothetical 40.0 kDa protein cy02b10.25c (396 aa), FASTA scores: opt: 2124, E(): 0, (85.1% identity in 397 aa overlap). Note that expression of Rv1196 was demonstrated in lysates by immunodetection (see Dillon et al., 1999). L7N675 No PE/PPE Q7D8M9 L7N675 L7N675 NC_000962.3 Mycobrowser_v5 CDS 1346936 1348624 . - 0 Rv1204c Rv1204c Function unknown Conserved hypothetical protein Rv1204c, (MTCI364.16c), len: 562 aa. Conserved hypothetical protein, some similarity to Q55103 CHO-ORF2 from streptomyces SP. (642 aa), FASTA scores: opt: 215, E(): 3.6e-06, (26.4% identity in 576 aa overlap). Contains PS00017 ATP/GTP-binding site motif A. O05305 No conserved hypotheticals O05305 GO:0017111,GO:0005524 O05305 NC_000962.3 Mycobrowser_v5 CDS 1359472 1360146 . - 0 Rv1216c Rv1216c Function unknown Probable conserved integral membrane protein Rv1216c, (MTCI364.28c), len: 224 aa. Probable conserved integral membrane protein, C-terminal region similar to Q49963|U1756P from Mycobacterium leprae (134 aa), FASTA scores: opt: 311, E(): 3.3e-15, (52.2% identity in 113 aa overlap). O05317 No cell wall and cell processes O05317 O05317 GO:0016021,GO:0004671,GO:0006481 O05317 NC_000962.3 Mycobrowser_v5 ncRNA 1340578 1340625 . + 0 MTB000075 ncrMT1234 Unknown Fragment of putative small regulatory RNA ncrMT1234, fragment of putative small regulatory RNA (See Pelly et al., 2012), cloned from M. tuberculosis CDC1551; supported by RNA-seq in H37Rv (unpublished data). No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 1331021 1332010 . + 0 Rv1188 Rv1188 Oxidizes proline to glutamate for use as a carbon and nitrogen source [catalytic activity: L-proline + acceptor + H2O = (S)-1-pyrroline-5-carboxylate + reduced acceptor] Probable proline dehydrogenase Rv1188, (MTV005.24), len: 329 aa. Possible putA, proline dehydrogenase, similar to part of Q52711|X78346 proline dehydrogenase from Rhodobacter capsulatus (1127 aa), FASTA scores: opt: 194, E(): 1.5e-07, (31.2% identity in 349 aa overlap). Also similar to two Bacillus subtilis proline dehydrohenases E1184363|Z99120 (302 aa), FASTA scores: opt: 509, E(): 0, (37.1% identity in 313 aa overlap); and E1182272|Z99105 (303 aa), FASTA scores: opt: 513, E(): 0, (32.5% identity in 311 aa overlap). Highly similar to AL035569|SC8D9.31 Streptomyces coelicolor (308 aa), FASTA scores: opt: 984, E(): 0, (50.0% identity in 312 aa overlap). Nucleotide position 1331696 in the genome sequence has been corrected, A:C resulting in R226R. O50444 No intermediary metabolism and respiration O50444 O50444 GO:0055114,GO:0006562,GO:0004657,GO:0006537 1.5.99.8 O50444 NC_000962.3 Mycobrowser_v5 CDS 1333931 1334845 . + 0 Rv1191 Rv1191 Function unknown Conserved protein Rv1191, (MTCI364.03), len: 304 aa. Conserved protein, similar to Q54528 RDMC from Streptomyces purpurascens (298 aa), FASTA scores: opt: 196, E(): 1.5e-05, (27.5% identity in 269 aa overlap); Rv0134|MTCI5.08 (300 aa), FASTA scores: opt: 197, E(): 6.6e-06, (26.4% identity in 299 aa overlap), some similarity to PIP_NEIGO|P42786 proline iminopeptidase (310 aa), FASTA scores: opt: 196, E(): 1.3e-05, (32.2% identity in 152 aa overlap). Contains PS00044 Bacterial regulatory proteins, lysR family signature. O05293 No conserved hypotheticals O05293 O05293 O05293 NC_000962.3 Mycobrowser_v5 CDS 1337248 1338513 . - 0 Rv1194c Rv1194c Function unknown Conserved protein Rv1194c, (MTCI364.06c), len: 421 aa. Conserved protein, highly similar to Q50018 possible transcriptional activator from Mycobacterium leprae (517 aa), FASTA scores: opt: 1960, E(): 0, (69.8% identity in 421 aa overlap). Also similar to Mycobacterium tuberculosis Rv2370c|MTCY27.10, (62.0% identity in 421 aa overlap) and Rv1453|MTCY493.01c. O05296 No conserved hypotheticals O05296 O05296 NC_000962.3 Mycobrowser_v5 CDS 1339003 1339302 . + 0 Rv1195 PE13 Function unknown PE family protein PE13 Rv1195, (MTCI364.07), len: 99 aa. PE13, Member of Mycobacterium tuberculosis PE family (see Brennan & Delogu 2002), e.g. Y0DP_MYCTU|Q50615 hypothetical glycine-rich 40.8 kd protein (498 aa), FASTA scores: opt: 307, E(): 1.4e-12, (56.3% identity in 96 aa overlap), similar to MTCY21C12.10c (99 aa), FASTA scores: opt:295, E(): 1.9e-11, (51.5% identity in 97 aa overlap). Q79FR3 No PE/PPE Q79FR3 Q79FR3 Q79FR3 NC_000962.3 Mycobrowser_v5 CDS 1342942 1344219 . + 0 Rv1200 Rv1200 Unknown; probably involved in transport accross the membrane (probably sugar transport). Probable conserved integral membrane transport protein Rv1200, (MTCI364.12), len: 425 aa. Probable conserved integral membrane transport protein, possibly member of major facilitator superfamily (MFS), similar to others e.g. YHJE_ECOLI|P37643 hypothetical metabolite transport protein from Escherichia coli (440 aa), FASTA scores: opt: 1047, E(): 0, (39.1% identity in 427 aa overlap); etc. Contains PS00217 Sugar transport proteins signature 2. The transcription of this CDS seems to be activated in macrophages (see citation below). O05301 No cell wall and cell processes O05301 O05301 GO:0016021,GO:0055085,GO:0005215,GO:0008643 NC_000962.3 Mycobrowser_v5 CDS 1344216 1345169 . - 0 Rv1201c dapD Involved in biosynthesis of diaminopimelate and lysine from aspartate semialdehyde (at the third step) [catalytic activity: tetrahydrodipicolinate + succinyl-CoA + H2O = N-succinyl-2-amino-6-ketopimelate + coenzyme A] Tetrahydrodipicolinate N-succinyltransferase DapD Rv1201c, (MTCI364.13c), len: 317 aa. dapD, tetrahydrodipicolinate N-succinyltransferase. Highly similar to Q49948|U1756F Mycobacterium leprae (317 aa), FASTA scores: opt: 1776, E(): 0, (84.9% identity in 317 aa overlap), also Q46064 ORF3 protein from corynebacterium glutamicum (316 aa), FASTA scores: opt: 864, E(): 0, (44.1% identity in 311 aa overlap). P9WP21 No intermediary metabolism and respiration 3FSX,3FSY P9WP21 GO:0008666 2.3.1.117 P9WP21 NC_000962.3 Mycobrowser_v5 CDS 1346321 1346905 . - 0 Rv1203c Rv1203c Unknown Hypothetical protein Rv1203c, (MTCI364.15c), len: 194 aa. Hypothetical unknown protein. O05304 No conserved hypotheticals O05304 O05304 NC_000962.3 Mycobrowser_v5 CDS 1348719 1349282 . + 0 Rv1205 Rv1205 Function unknown Conserved hypothetical protein Rv1205, (MTCI364.17), len: 187 aa. Conserved hypothetical protein, similar to Q49952 cosmid B1756 from Mycobacterium leprae (187 aa), FASTA scores: opt: 865, E(): 0, (72.4% identity in 174 aa overlap), also similar to FAS6_RHOFA|P46378 hypothetical 21.1 kDa protein in fasciation locus (ORF6) (198 aa), FASTA scores: opt: 368, E(): 1.3e-17, (37.4% identity in 174 aa overlap). Some similarity to YJL055W Hypothetical protein in BTN1-PEP8 intergenic region from Saccharomyces cerevisiae and P48636 hypothetical protein in AZU 5'region from Pseudomonas aeruginosa. The transcription of this CDS seems to be activated specifically in host granulomas (see citation below). O05306 No conserved hypotheticals O05306 O05306 O05306 NC_000962.3 Mycobrowser_v5 CDS 1351191 1352147 . + 0 Rv1207 folP2 Involved in dihydrofolate biosynthesis [catalytic activity: 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate] Dihydropteroate synthase 2 FolP2 (DHPS 2) (dihydropteroate pyrophosphorylase 2) Rv1207, (MTCI364.19), len: 318 aa. folP2, Dihydropteroate synthase 2, similar to many e.g. DHPS_ECOLI|P26282 Escherichia coli (282 aa), FASTA scores: opt: 480, E(): 1.9e-22, (34.4% identity in 270 aa overlap). Contains PS00792 dihydropteroate synthase signature 1, PS00793 dihydropteroate synthase signature 2. P9WNC9 No intermediary metabolism and respiration 2VP8 P64139 P9WNC9 GO:0046656,GO:0046677,GO:0004156 2.5.1.15 P9WNC9 NC_000962.3 Mycobrowser_v5 CDS 1352144 1353118 . + 0 Rv1208 gpgS Glucosylglycerate biosynthesis [catalytic activity: NDP-glucose + 3-phosphoglycerate = glucosyl-3-phsophoglycerate + NDP] Probable glucosyl-3-phosphoglycerate synthase GpgS Rv1208, (MTCI364.20), len: 324 aa. Probable gpgS, glucosyl-3-phosphoglycerate synthase (See Empadinhas et al., 2008), similar to Q49955|U1756L Mycobacterium leprae (318 aa), FASTA scores, opt: 1621, E(): 0, (80.5% identity in 318 aa overlap). Belongs to retaining glycosyltransferase family 81. P9WMW9 No intermediary metabolism and respiration 3E25,3E26 O05309 P9WMW9 P9WMW9 NC_000962.3 Mycobrowser_v5 CDS 1353522 1354136 . + 0 Rv1210 tagA Involved in base excision repair. Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions Probable DNA-3-methyladenine glycosylase I TagA (tag I) (3-methyladenine-DNA glycosylase I, constitutive) (DNA-3-methyladenine glycosidase I) Rv1210, (MTCI364.22), len: 204 aa. Probable tagA, DNA-3-methyladenine glycosidase I (see citation below), similar to several e.g. 3MG1_ECOLI|P05100 DNA-3-methyladenine glycosidase I from Escherichia coli (187 aa), FASTA scores: opt: 530, E(): 1.3e-27, (44.2% identity in 190 aa overlap); similar to Q49957 Mycobacterium leprae cosmid B1756 (192 aa), FASTA scores: opt: 1042, E(): 0, (80.2% identity in 192 aa overlap). O05311 No information pathways O05311 O05311 GO:0006284,GO:0008725 3.2.2.20 O05311 NC_000962.3 Mycobrowser_v5 CDS 1354243 1354470 . + 0 Rv1211 Rv1211 Function unknown Conserved protein Rv1211, (MTCI364.23), len: 75 aa. Conserved protein, similar to Q49958|U1756N Mycobacterium leprae (75 aa), FASTA scores: opt: 460, E(): 0, (90.7% identity in 75 aa overlap). O05312 No conserved hypotheticals O05312 O05312 O05312 NC_000962.3 Mycobrowser_v5 CDS 1354498 1355661 . - 0 Rv1212c glgA Function unknown; probably involved in cellular metabolism Putative glycosyl transferase GlgA Rv1212c, (MTCI364.24c), len: 387 aa. Putative glgA, glycosyl transferase, highly similar to AJ243803|SCO243803_2 Putative glycosyl transferase from Streptomyces coelicolor (387 aa), FASTA scores: opt: 1344, E(): 0, (54.9% identity in 388 aa overlap). Also similar to MJ1607 probable hexosyltransferase from Methanococcus jannaschii (390 aa), FASTA scores: opt: 445, E(): 7.8e-23, (27.9% identity in 401 aa overlap). The region from aa 267-355 highly similar to Q49959 cosmid B1756 from Mycobacterium leprae (91 aa), FASTA scores, opt: 471, E(): 4.8e-25, (80.9% identity in 89 aa overlap). Similar to Mycobacterium tuberculosis hypothetical protein, Rv3032. P9WMZ1 No intermediary metabolism and respiration O05313 P9WMZ1 GO:0016740,GO:0009058 2.-.-.- P9WMZ1 NC_000962.3 Mycobrowser_v5 CDS 1363503 1364150 . - 0 Rv1220c Rv1220c Function unknown; involved in cellular metabolism Probable methyltransferase Rv1220c, (MTCI61.03c), len: 215 aa. Possible methyltransferase, some similarity to MDMC_STRMY|Q00719 o-methyltransferase from Streptomyces mycarofaciens (221 aa), FASTA scores; opt: 289, E(): 1.3e-07, (30.0% identity in 203 aa overlap). Also similar to Mycobacterium tuberculosis methyltransferases Rv0187|MTCI28.26 (32.9% identity in 222 aa overlap) and Rv1703c. Start site chosen by homology; other possible start sites exist upstream. P9WJZ7 No intermediary metabolism and respiration O33219 P9WJZ7 GO:0008171 2.1.1.- P9WJZ7 NC_000962.3 Mycobrowser_v5 CDS 1374322 1374864 . - 0 Rv1231c Rv1231c Unknown Probable membrane protein Rv1231c, (MTV006.03c), len: 180 aa. Probable membrane protein, similar to others e.g. AL390975 Streptomyces coelicolor (198 aa). O86314 No cell wall and cell processes O86314 O86314 NC_000962.3 Mycobrowser_v5 CDS 2259326 2259820 . + 0 Rv2012 Rv2012 Unknown Conserved hypothetical protein Rv2012, (MTCY39.05c), len: 164 aa. Conserved hypothetical protein. P9WLM3 No conserved hypotheticals P9WLM3 P9WLM3 NC_000962.3 Mycobrowser_v5 ncRNA 1365274 1365365 . + 0 MTB000062 mpr6 Unknown Fragment of putative small regulatory RNA mpr6, fragment of putative small regulatory RNA (See DiChiara et al., 2010), ends not mapped, ~118 nt band detected by Northern blot in M. bovis BCG Pasteur. No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1413094 1413224 . - 0 MTB000063 mcr11 Unknown Putative small regulatory RNA mcr11, putative small regulatory RNA (See DiChiara et al., 2010). 5'-end mapped by RLM-RACE in M. tuberculosis H37Rv, 3'-end not mapped (See Arnvig et al., 2011). No stable RNAs NC_000962.3 Mycobrowser_v5 -10_signal 1413232 1413237 . - 0 MTB000063 mcr11 Unknown Putative small regulatory RNA mcr11, putative small regulatory RNA (See DiChiara et al., 2010). 5'-end mapped by RLM-RACE in M. tuberculosis H37Rv, 3'-end not mapped (See Arnvig et al., 2011). No stable RNAs NC_000962.3 Mycobrowser_v5 -35_signal 1413255 1413260 . - 0 MTB000063 mcr11 Unknown Putative small regulatory RNA mcr11, putative small regulatory RNA (See DiChiara et al., 2010). 5'-end mapped by RLM-RACE in M. tuberculosis H37Rv, 3'-end not mapped (See Arnvig et al., 2011). No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 1361798 1362733 . - 0 Rv1218c Rv1218c Thought to be involved in active transport of tetronasin across the membrane (export): tetronasin resistance by an export mechanism. Responsible for energy coupling to the transport system. Probable tetronasin-transport ATP-binding protein ABC transporter Rv1218c, (MTCI61.01c), len: 311 aa. Probable tetronasin-transport ATP-binding protein ABC transporter (see citation below), similar to many e.g. Q54406|X73633|TNRB2 TNRB2 protein from Streptomyces longisporoflavus (300 aa), FASTA scores: opt: 1133, E(): 0, (60.8% identity in 291 aa overlap); etc. Also similar to others in Mycobacterium tuberculosis e.g. MTCY19H9.04 (30.0% identity in 297 aa overlap); etc. Contains PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters). O86311 No cell wall and cell processes O86311 O86311 GO:0016887,GO:0005524 O86311 NC_000962.3 Mycobrowser_v5 CDS 1362723 1363361 . - 0 Rv1219c Rv1219c Involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv1219c, (MTCI61.02c), len: 212 aa. Probable transcriptional regulatory protein, some similarity in N-terminus to YBIH_ECOLI|P41037 hypothetical transcriptional regulator from Escherichia coli (103 aa), FASTA scores: opt: 143, E(): 8.9e-06, (39.7% identity in 63 aa overlap); Helix turn helix motif from aa 28-49. O86312 No regulatory proteins O86312 O86312 GO:0006350,GO:0003700,GO:0006355 O86312 NC_000962.3 Mycobrowser_v5 -10_signal 1363369 1363374 . - 0 Rv1219c Rv1219c Involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv1219c, (MTCI61.02c), len: 212 aa. Probable transcriptional regulatory protein, some similarity in N-terminus to YBIH_ECOLI|P41037 hypothetical transcriptional regulator from Escherichia coli (103 aa), FASTA scores: opt: 143, E(): 8.9e-06, (39.7% identity in 63 aa overlap); Helix turn helix motif from aa 28-49. O86312 No regulatory proteins O86312 O86312 GO:0006350,GO:0003700,GO:0006355 O86312 NC_000962.3 Mycobrowser_v5 -35_signal 1363392 1363397 . - 0 Rv1219c Rv1219c Involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv1219c, (MTCI61.02c), len: 212 aa. Probable transcriptional regulatory protein, some similarity in N-terminus to YBIH_ECOLI|P41037 hypothetical transcriptional regulator from Escherichia coli (103 aa), FASTA scores: opt: 143, E(): 8.9e-06, (39.7% identity in 63 aa overlap); Helix turn helix motif from aa 28-49. O86312 No regulatory proteins O86312 O86312 GO:0006350,GO:0003700,GO:0006355 O86312 NC_000962.3 Mycobrowser_v5 CDS 1413260 1413940 . + 0 Rv1265 Rv1265 Unknown. Seems to be expressed during macrophage infection. Unknown protein Rv1265, (MTCY50.17c), len: 226 aa. Unknown protein (see citation below). P9WM49 No conserved hypotheticals P9WM49 NC_000962.3 Mycobrowser_v5 CDS 1367463 1367858 . + 0 Rv1224 tatB Involved in proteins export. This sec-independent pathway is termed tat for twin-arginine translocation system. This system mainly transports proteins with bound cofactors that require folding prior to export. Probable protein TatB Rv1224, (MTCI61.07), len: 131 aa. Probable tatB, component of twin-arginine translocation protein export system (see citation below). Possible exported protein with hydrophobic stretch at N-terminus. Highly similar to Q49973|U15180 hypothetical protein U1756Y from Mycobacterium leprae (120 aa), FASTA scores: opt: 601, E(): 0, (73.3% identity in 131 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WG99 No cell wall and cell processes O33220 P9WG99 GO:0005886,GO:0009306,GO:0043953,GO:0008565,GO:0055085,GO:0016021 P9WG99 NC_000962.3 Mycobrowser_v5 CDS 1368832 1370295 . - 0 Rv1226c Rv1226c Unknown Probable transmembrane protein Rv1226c, (MTCI61.09c), len: 487 aa. Probable transmembrane protein. Some similarity to AL049841|SCE9.01 Streptomyces coelicolor (436 aa), FASTA scores: opt: 203, E(): 1.2e-05, (29.8% identity in 346 aa overlap). O33222 No cell wall and cell processes O33222 O33222 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1370292 1370825 . - 0 Rv1227c Rv1227c Unknown Probable transmembrane protein Rv1227c, (MTCI61.10c), len: 177 aa. Possible transmembrane protein, similar to P96615 hypothetical protein ydbS from Bacillus subtilis (159 aa), fasta scores: E(): 3.6e-07, (30.1% identity in 163 aa overlap). O33223 No cell wall and cell processes O33223 O33223 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1371777 1372949 . - 0 Rv1229c mrp Unknown: thought to be a ATP-binding protein. Probable Mrp-related protein Mrp Rv1229c, (MT1267, MTCI61.12c, MTV006.01c), len: 390 aa. Probable Mrp protein, similar to others e.g. MRP_ECOLI|P21590 mrp protein from Escherichia coli (379 aa), FASTA scores: E(): 0, (34.1% identity in 355 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop); and PS01215 MRP Prosite domain. Belongs to the MRP/NBP35 family of ATP-binding proteins. P9WJN7 No intermediary metabolism and respiration P65441 P9WJN7 GO:0005524 P9WJN7 NC_000962.3 Mycobrowser_v5 CDS 1372962 1374197 . - 0 Rv1230c Rv1230c Unknown Possible membrane protein Rv1230c, (MTV006.02c), len: 411 aa. Possible membrane protein with two hydrophobic stretches near N-terminus. Some similarity to Rv1022|MTCY10G2.27c|Z92539 probable lpqU protein Mycobacterium tuberculosis (243 aa), FASTA score: opt: 408, E(): 1e-11, (43.6% identity in 172 aa overlap). Similar to AL133423|SC4A7.37 hypothetical protein from Streptomyces coelicolor (421 aa), FASTA score: opt: 679, E(): 5.1e-23, (36.4% identity in 398 aa overlap). O86313 No cell wall and cell processes O86313 GO:0003700,GO:0006355 O86313 NC_000962.3 Mycobrowser_v5 CDS 1376230 1376826 . - 0 Rv1233c Rv1233c Function unknown Conserved hypothetical membrane protein Rv1233c, (MTV006.05c), len: 198 aa. Conserved hypothetical membrane protein, N-terminus is highly proline rich, C-terminus has two hydrophobic stretches. Proline-rich N-terminus has some similarity to CBPA_DICDI calcium binding protein from Dictyostelium discoideum (467 aa), FASTA scores: E(): 4.8e-06, (35.5% identity in 183 aa overlap). Both sequences share multiple copies of a Tyr-Pro-Pro motif. O86316 No cell wall and cell processes O86316 NC_000962.3 Mycobrowser_v5 CDS 1376976 1377503 . + 0 Rv1234 Rv1234 Unknown Probable transmembrane protein Rv1234, (MTV006.06), len: 175 aa. Possible transmembrane protein with two TM helices. O50451 No cell wall and cell processes O50451 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1378927 1379850 . + 0 Rv1236 sugA Involved in active transport of sugar across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable sugar-transport integral membrane protein ABC transporter SugA Rv1236, (MTV006.08), len: 307 aa. Probable sugA, sugar-transport integral membrane protein ABC transporter (see citation below), equivalent to U15180|MLU1518035 protein malFM from Mycobacterium leprae (310 aa), FASTA scores: opt: 1566, E(): 0, (81.8% identity in 292 aa overlap). Also similar to numerous bacterial sugar transport system components. Also similar to Rv2316|MTCY3G12.18c from Mycobacterium tuberculosis (290 aa), FASTA scores: opt: 514, E(): 7.3e-27, (33.2% identity in 283 aa overlap). Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature. P9WG03 No cell wall and cell processes O50452 P9WG03 GO:0005886,GO:0006810,GO:0005215,GO:0016021 P9WG03 NC_000962.3 Mycobrowser_v5 CDS 1386857 1387717 . + 0 Rv1244 lpqZ Unknown Probable lipoprotein LpqZ Rv1244, (MTV006.16), len: 286 aa. Probable lipoprotein lpqZ, equivalent toU15180|MLU1518042 protein u1756x from Mycobacterium leprae (228 aa), FASTA scores: opt: 1039, E(): 0, (72.5% identity in 229 aa overlap). Similar to Mycobacterium tuberculosis hypothetical protein Rv3759c. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O50459 No cell wall and cell processes O50459 O50459 GO:0006810,GO:0005215 O50459 NC_000962.3 Mycobrowser_v5 CDS 1379855 1380679 . + 0 Rv1237 sugB Involved in active transport of sugar across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable sugar-transport integral membrane protein ABC transporter SugB Rv1237, (MTV006.09), len: 274 aa. Probable sugB, sugar-transport integral membrane protein ABC transporter (see citation below), equivalent to U15180|MLU1518036 protein MalGM from Mycobacterium leprae (296 aa), FASTA scores: opt: 1571, E(): 0, (89.8% identity in 274 aa overlap). Also similar to numerous bacterial sugar transport protein. Related to Rv2834c|MTCY16B7.08 from Mycobacterium tuberculosis (275 aa), FASTA scores: opt: 370, E(): 2.4e-17, (26.8% identity in 269 aa overlap). P9WG01 No cell wall and cell processes O50453 P9WG01 GO:0005886,GO:0006810,GO:0005215,GO:0016021 P9WG01 NC_000962.3 Mycobrowser_v5 CDS 1456565 1457542 . + 0 Rv1300 hemK Possibly involved in the oxidation of protoporphyrinogen into protoporphyrin IX Probable HemK protein homolog HemK Rv1300, (MTCY373.20), len: 325 aa. Probable hemK protein homolog, homology suggests translation may start at aa 22, highly similar to many e.g. HEMK_MYCLE|P45832 Mycobacterium leprae (288 aa), FASTA scores: opt: 936, E(): 0, (76.7% identity in 189 aa overlap). Belongs to the HemK family of modification methylases. P9WHV3 No intermediary metabolism and respiration Q10602 P9WHV3 GO:0006479,GO:0008276,GO:0003676 2.1.1.- P9WHV3 NC_000962.3 Mycobrowser_v5 CDS 1686570 1686887 . + 0 Rv1495 mazF4 Sequence-specific mRNA cleavage Possible toxin MazF4 Rv1495, (MTCY277.17), len: 105 aa. Possible mazF4, toxin, part of toxin-antitoxin (TA) operon with Rv1494 (See Pandey and Gerdes, 2005; Zhu et al., 2006), some similarity to Rv1942c|MTCY09F9.22 hypothetical protein from Mycobacterium tuberculosis (109 aa) (0.7% identity in 101 aa overlap) and Rv0659c, Rv1102c. P9WII5 No virulence, detoxification, adaptation P64859 P9WII5 GO:0003677 P9WII5 NC_000962.3 Mycobrowser_v5 CDS 1384278 1384538 . + 0 Rv1241 vapB33 Unknown Possible antitoxin VapB33 Rv1241, (MTV006.13), len: 86 aa. Possible vapB33, antitoxin, part of toxin-antitoxin (TA) operon with Rv1242, see Arcus et al. 2005. Member of family of 16 hypothetical Mycobacterium tuberculosis proteins including: Rv2871|Q10799|YS71_MYCTU hypothetical 13.2 kDa protein CY2 (124 aa), FASTA scores: opt: 172, E(): 9.5e-06, (37.2% identity in 86 aa overlap); Rv2132, Rv3321c, etc. O50456 No virulence, detoxification, adaptation O50456 O50456 NC_000962.3 Mycobrowser_v5 CDS 1381942 1383042 . - 0 Rv1239c corA Thought to be involved in transport of magnesium and cobalt ions across the membrane. Responsible for the translocation of the substrate across the membrane. Possible magnesium and cobalt transport transmembrane protein CorA Rv1239c, (MTV006.11c), len: 366 aa. Possible corA, magnesium and cobalt transport transmembrane protein, highly similar to U15180 corA protein from Mycobacterium leprae (373 aa), FASTA scores: opt: 1985, E(): 0, (79.1% identity in 369 aa overlap). Also similar to various CorA proteins of Gram negative bacteria e.g. P27841|CORA_ECOLI|B3816|Z5333|ECS4746 Magnesium and cobalt transport protein from Escherichia coli strains K12 and O157:H7 (316 aa), FASTA scores: opt: 236, E(): 8e-08, (24.5% identity in 306 aa overlap); etc. Seems to belong to the MIT family. O50455 No cell wall and cell processes O50455 O50455 GO:0006824,GO:0016021,GO:0015095,GO:0015693,GO:0055085,GO:0015087 O50455 NC_000962.3 Mycobrowser_v5 CDS 1383213 1384202 . + 0 Rv1240 mdh Involved in the conversion of malate to oxaloacetate [catalytic activity: (S)-malate + NAD+ = oxaloacetate + NADH]. Probable malate dehydrogenase Mdh Rv1240, (MTV006.12), len: 329 aa. Probable mdh, Malate dehydrogenase. Most similar to P50917|MDH_MYCLE malate dehydrogenase from Mycobacterium leprae (329 aa), FASTA scores: opt: 1887, E(): 0, (89.1% identity in 329 aa overlap). Contains PS00068 Malate dehydrogenase active site signature. Belongs to the LDH family. MDH subfamily. P9WK13 No intermediary metabolism and respiration P0A5J6 P9WK13 GO:0006096,GO:0006108,GO:0055114,GO:0006099,GO:0030060 1.1.1.37 P9WK13 NC_000962.3 Mycobrowser_v5 CDS 1384535 1384966 . + 0 Rv1242 vapC33 Unknown Possible toxin VapC33. Contains PIN domain. Rv1242, (MTV006.14), len: 143 aa. Possible vapC33, toxin, part of toxin-antitoxin (TA) operon with Rv1241, contains PIN domain, see Arcus et al. 2005. Member of family of 14 hypothetical Mycobacterium tuberculosis proteins including: Rv2872|Q10800|YS72_MYCTU (147 aa), FASTA scores: opt: 226, E(): 2.7e-09, (32.1% identity in 137 aa overlap); Rv0749, Rv0277c, Rv2530c, etc. P9WF69 No virulence, detoxification, adaptation P9WF69 P9WF69 NC_000962.3 Mycobrowser_v5 CDS 1384989 1386677 . - 0 Rv1243c PE_PGRS23 Function unknown PE-PGRS family protein PE_PGRS23 Rv1243c, (MTV006.15c), len: 562 aa. PE_PGRS23, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002). Q79FQ7 No PE/PPE Q79FQ7 Q79FQ7 Q79FQ7 NC_000962.3 Mycobrowser_v5 CDS 1699866 1700228 . + 0 Rv1508A Rv1508A Function unknown Conserved hypothetical protein Rv1508A, len: 120 aa. Conserved hypothetical protein, highly similar to central part of glycosyl transferases from various mycobacteria and eubacteria e.g. P71790|MTCY277.33|Rv1511 Hypothetical protein from M. tuberculosis (340 aa), FASTA scores: opt: 210, E(): 2.5 e-09, (42.9% identity in 105 aa overlap). Q79FN0 No conserved hypotheticals Q79FN0 GO:0019673,GO:0005622,GO:0008446 Q79FN0 NC_000962.3 Mycobrowser_v5 CDS 1388975 1389244 . - 0 Rv1247c relB Function unknown Antitoxin RelB Rv1247c, (MTV006.19c), len: 89 aa. RelB, antitoxin, part of toxin-antitoxin (TA) operon with Rv1246c (See Pandey and Gerdes, 2005), some similarity to hypothetical proteins including Mycobacterium tuberculosis proteins Rv2865|MTV003.11 (93 aa), FASTA scores: opt: 249, E(): 5.4e-13, (44.2% identity in 86 aa overlap); Rv0268|Z86089|P95225 (169 aa) opt: 125, E(): 0.0089, (41.8% identity in 55 aa overlap); etc. and Escherichia coli AE000293|ECAE0002933 (92 aa), FASTA scores: opt: 127, E(): 0.0038, (29.3% identity in 82 aa overlap). O50462 No virulence, detoxification, adaptation O50462 O50462 O50462 NC_000962.3 Mycobrowser_v5 CDS 1393194 1393982 . - 0 Rv1249c Rv1249c Unknown Possible membrane protein Rv1249c, (MTV006.21c), len: 262 aa. Possible membrane protein. Start uncertain. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O50464 No cell wall and cell processes O50464 NC_000962.3 Mycobrowser_v5 CDS 1399970 1401661 . + 0 Rv1253 deaD Has a helix-destabilizing activity Probable cold-shock DeaD-box protein A homolog DeaD (ATP-dependent RNA helicase dead homolog) Rv1253, (MTCY50.29c), len: 563 aa. Probable deaD, Cold-shock dead-box protein A homolog, similar to many e.g. DEAD_ECOLI|P23304 Escherichia coli (646 aa), FASTA scores: opt: 1490, E(): 0, (46.7% identity in 578 aa overlap); similar to Mycobacterium tuberculosis Rv3211. Contains PS00017 ATP/GTP-binding site motif A, PS00039 dead-box subfamily ATP-dependent helicases signature. Belongs to the dead box family helicase. P9WH05 No information pathways Q11039 P9WH05 GO:0008026,GO:0003723,GO:0005737,GO:0045449,GO:0006350,GO:0005524 3.6.4.13 P9WH05 NC_000962.3 Mycobrowser_v5 CDS 1700212 1701093 . + 0 Rv1509 Rv1509 Unknown Hypothetical protein Rv1509, (MTCY277.31), len: 293 aa. Hypothetical unknown protein. P9WLW3 No unknown P71788 P9WLW3 GO:0008168,GO:0008152 P9WLW3 NC_000962.3 Mycobrowser_v5 CDS 1764755 1764973 . + 0 Rv1560 vapB11 Unknown Possible antitoxin VapB11 Rv1560, (MTCY48.05c), len: 72 aa. Possible vapB11, antitoxin, part of toxin-antitoxin (TA) operon with Rv1561 (See Arcus et al., 2005; Pandey and Gerdes, 2005). Part of a Mycobacterial tuberculosis family of proteins e.g. Q10848|Rv2009|MTCY39.08c (80 aa), FASTA score: (54.4% identity in 68 aa overlap); Q10799|Rv2871|MTCY274.02 (85 aa); O50456|Rv1241|MTV006.13 (86 aa), O06243|Rv2132|MTCY270.36C (76 aa); etc. P9WLU3 No virulence, detoxification, adaptation P64877 P9WLU3 P9WLU3 NC_000962.3 Mycobrowser_v5 CDS 1395821 1399240 . - 0 Rv1251c Rv1251c Function unknown Conserved hypothetical protein Rv1251c, (MTV006.23c), len: 1139 aa. Conserved hypothetical protein, showing some similarity in C-terminal region with other proteins from eukaryotes and bacteria e.g. NP_142121.1 hypothetical protein from Pyrococcus horikoshii (1188 aa); and some similarity to GTP-binding proteins e.g. P23249|MV10_MOUSE putative GTP-binding protein (1004 aa), FASTA scores: opt: 228, E(): 1.7e-06, (27.7% identity in 560 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O50466 No conserved hypotheticals O50466 O50466 GO:0017111,GO:0005524 O50466 NC_000962.3 Mycobrowser_v5 CDS 1399296 1399904 . - 0 Rv1252c lprE Unknown Probable lipoprotein LprE Rv1252c, (MTCY50.30), len: 202 aa. Probable lipoprotein lprE, some similarity to Mycobacterium tuberculosis protein Rv3483c|MTCY13E12.36C (220 aa), FASTA scores: E(): 7e-05, (29.5% identity in 200 aa overlap). Contains possible N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein lipid attachment site (PS00013). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WK49 No cell wall and cell processes P9WK49 GO:0005886 NC_000962.3 Mycobrowser_v5 CDS 1401658 1402809 . + 0 Rv1254 Rv1254 Catalyzes the acylation of the mycaminose sugar during midecamycin biosynthesis Probable acyltransferase Rv1254, (MTCY50.28c), len: 383 aa. Probable Acyltransferase, similar to G927228 midecamycin 4-0-propionyl transferase (fragment) (388 aa), FASTA scores, opt: 305, E(): 5.6e-14, (28.4% identity in 377 aa overlap). Also similar to other Mycobacterium tuberculosis acyltransferases e.g. Rv0111, Rv0228, etc. Contains PS00881 Protein splicing signature. Q11064 No intermediary metabolism and respiration Q11064 Q11064 GO:0016020,GO:0008415 2.3.1.- Q11064 NC_000962.3 Mycobrowser_v5 CDS 1403386 1404603 . - 0 Rv1256c cyp130 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Probable cytochrome P450 130 Cyp130 Rv1256c, (MT1295, MTCY50.26), len: 405 aa. Probable cyp130, cytochrome P450, similar to other cytochromes P-450 e.g. S51594 cytochrome P450 mycG from Micromonospora griseorubida (397 aa); T36526 probable cytochrome P450 hydroxylase from Streptomyces coelicolor (411 aa); CPXK_SACER|P33271|107B1 cytochrome P450 from Saccharopolyspora erythraea (405 aa), FASTA scores: opt: 639, E(): 2.7e-33, (33.2% identity in 391 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. Rv0766c|MTCY369.11c cytochrome P450 (402 aa); etc. Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. Belongs to the cytochrome P450 family. P9WPN5 No intermediary metabolism and respiration 2UUQ,2UVN,2WGY,2WH8,2WHF Q11062 P9WPN5 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPN5 NC_000962.3 Mycobrowser_v5 CDS 1404717 1406084 . - 0 Rv1257c Rv1257c Function unknown; probably involved in cellular metabolism Probable oxidoreductase Rv1257c, (MTCY50.25), len: 455 aa. Probable oxidoreductase, similar to e.g. GLCD_ECOLI|P52075 glycolate oxidase subunit glcd (499 aa), FASTA scores: E(): 0, (38.9% identity in 458 aa overlap). Similar to Mycobacterium tuberculosis oxidoreductases e.g. Rv3107c Q11061 No intermediary metabolism and respiration Q11061 Q11061 GO:0055114,GO:0016491,GO:0050660 1.-.-.- Q11061 NC_000962.3 Mycobrowser_v5 CDS 1407339 1408238 . + 0 Rv1259 udgB Involved in base excision repair. Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. Probable uracil DNA glycosylase, UdgB Rv1259, (MTCY50.23c), len: 299 aa. Probable udgB, uracil DNA glycosylase. Similar to AL109732|SC7H2.04 hypothetical protein from Streptomyces coelicolor (237 aa), FASTA scores: opt: 870, E(): 0, (57.1% identity in 231 aa overlap). P9WM53 No information pathways P64785 P9WM53 P9WM53 NC_000962.3 Mycobrowser_v5 CDS 1409484 1409933 . - 0 Rv1261c Rv1261c Function unknown Conserved protein Rv1261c, (MTCY50.21), len: 149 aa. Conserved protein, similar to Mycobacterium tuberculosis hypothetical proteins e.g. Rv1558|MTCY48.07c (39.2% identity in 125 aa overlap); Rv3547 and Rv3178. P9WP13 No conserved hypotheticals P64787 P9WP13 P9WP13 NC_000962.3 Mycobrowser_v5 CDS 1409938 1410372 . - 0 Rv1262c Rv1262c Unknown Hypothetical hit-like protein Rv1262c, (MTCY50.20), len: 144 aa. Hypothetical hit-like protein, similar to Q04344|HIT_YEAST hit1 protein (orf u) (144 aa), FASTA scores: opt: 306, E(): 3e-14, (35.9 % identity in 142 aa overlap); also similar to YHIT_MYCGE|P47378 hypothetical 15.6 kDa protein (141 aa), FASTA scores: opt: 250, E(): 1.6e-10, (35.5% identity in 107 aa overlap); and YHIT_MYCLE|P49774 hypothetical 17.0 kDa protein hit-like (155 aa), FASTA scores: opt: 196, E(): 7e-07, (30.6% identity in 144 aa overlap). Similar to other proteins from Mycobacterium tuberculosis e.g. Rv2613c, Rv0759c. Contains PS00892 hit family signature. Belongs to the hit family. P9WML1 No conserved hypotheticals Q11066 P9WML1 GO:0003824 P9WML1 NC_000962.3 Mycobrowser_v5 CDS 1410431 1411819 . + 0 Rv1263 amiB2 Involved in cellular metabolism, active on 2- to 6- carbon aliphatic amides and on many aromatic amides [catalytic activity: a monocarboxylic acid amide + H(2)O = a monocarboxylate + NH(3)]. Probable amidase AmiB2 (aminohydrolase) Rv1263, (MTCY50.19c), len: 462 aa. Probable amiB2, amidase. Similar to G1001278 hypothetical 54.3 kDa protein (506 aa), FASTA scores: opt: 767, E(): 7.6e-40, (32.8% identity in 461 aa overlap), also similar to G580673 rhodococcus enantiose lective amidase gene (462 aa), FASTA scores, opt: 668, E(): 7.4e-34, (33.5% identity in 484 aa overlap) also to NYLA_PSES8|P13398 6-aminohexanoate-cyclic-dimer hydrolase (492 aa), FASTA scores opt: 543, E(): 3.1e-26, (33.5% identity in 493 aa overlap). Also similar to MTCY274.19c (33.5% identity in 427 aa overlap). Similar to other putative amidases in M. tuberculosis; Rv2363, Rv2888c, etc. Contains PS00017 ATP/GTP-binding site motif A. Belongs to the amidase family. P9WQ97 No intermediary metabolism and respiration P63492 P9WQ97 GO:0016884,GO:0004040 3.5.1.4 P9WQ97 NC_000962.3 Mycobrowser_v5 CDS 1508543 1508923 . - 0 Rv1343c lprD Unknown Probable conserved lipoprotein LprD Rv1343c, (MTCY02B10.07c), len: 126 aa. Probable lprD, conserved lipoprotein, highly similar to G466928 Mycobacterium leprae protein B1549_F3_106 (126 aa), FASTA scores, opt: 704, E(): 7.5e-36, (78.4 % identity in 125 aa overlap). Has N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein attachment site. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WK51 No cell wall and cell processes P9WK51 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1416181 1417347 . - 0 Rv1267c embR Involved in transcriptional mechanism. Thought to regulate the biosynthesis of the mycobacterial cell wall arabinan and resistance to ethambutol (EMB; dextro-2,2'-(ethylenediimino)-DI-1-butanol), regulating EMBA|Rv3794 and EMBB|Rv3795. Probable transcriptional regulatory protein EmbR Rv1267c, (MT1305, MTCY50.15), len: 388 aa. Probable embR, regulatory protein (see citation below), similar to many e.g. AFSR_STRCO|P25941 regulatory protein AfsR from Streptomyces coelicolor (993 aa), FASTA scores: opt: 489, E(): 1e-25, (33.5% identity in 361 aa overlap); etc. Belongs to the AFSR/DNRI/REDD family of regulators. Phosphorylated in vitro by PknJ|Rv2088 (See Jang et al., 2010). P9WGJ9 No regulatory proteins 2FEZ,2FF4 P66799 P9WGJ9 GO:0005622,GO:0006355,GO:0006350,GO:0000156,GO:0000160,GO:0003677 EMB P9WGJ9 NC_000962.3 Mycobrowser_v5 CDS 1420410 1422305 . - 0 Rv1272c Rv1272c Thought to be involved in active transport of drugs across the membrane (export): multidrugs resistance by an export mechanism. Responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane. Probable drugs-transport transmembrane ATP-binding protein ABC transporter Rv1272c, (MTCY50.10), len: 631 aa. Probable drugs-transport transmembrane ATP-binding protein ABC transporter (see citation below), similar to e.g. Y015_MYCGE|P47261 hypothetical ABC transporter mg015m from Mycoplasma genitalium (589 aa), FASTA scores: opt: 1054, E(): 0, (34.3% identity in 522 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop); and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters), MSBA subfamily. P9WQJ3 No cell wall and cell processes P63397 P9WQJ3 GO:0042626,GO:0016021,GO:0005886,GO:0055085,GO:0005524 P9WQJ3 NC_000962.3 Mycobrowser_v5 CDS 1437324 1437815 . + 0 Rv1284 canA Catalyzes reversible dehydration of CO2 to form bicarbonate Beta-carbonic anhydrase Rv1284, (MTCY373.03), len: 163 aa. CanA, Beta-carbonic anhydrase, proven biochemically (See Suarez Covarrubias et al. 2005) similar to others e.g. AL109663|SC4A10.26 hypothetical protein from Streptomyces coelicolor (167 aa), FASTA scores: opt: 567, E(): 1.5e-32, (53.4% identity in 163 aa overla); shows some similarity to hypothetical protein from Methanobacterium thermoautotrophicum. Weak similarity to carbonic anhydrases e.g. U51624|MTU516242|P17582 Methanothermobacter thermautotrophicus (171 aa), FASTA score: opt: 305, E(): 1.2e-14, (35.2% identity in 165 aa overlap). P9WPJ7 No intermediary metabolism and respiration 1YLK P64797 P9WPJ7 GO:0004089,GO:0008270,GO:0015976 P9WPJ7 NC_000962.3 Mycobrowser_v5 CDS 1457558 1458211 . + 0 Rv1301 Rv1301 Function unknown Conserved protein Rv1301, (MTCY373.21), len: 217 aa. Conserved protein, highly similar to YRFE_MYCLE|P45831 hypothetical 22.7 kDa protein in rfe-hemk intergenic region, (220 aa), FASTA scores: opt: 1168, E(): 0, (82.8% identity in 215 aa overlap). Contains PS01147 Hypothetical SUA5/yciO/yrdC family signature. Belongs to the SUA5/YRDC/YCIO/YWLC family. P9WGC9 No conserved hypotheticals Q10618 P9WGC9 P9WGC9 NC_000962.3 Mycobrowser_v5 CDS 1413960 1415840 . - 0 Rv1266c pknH Involved in signal transduction (via phosphorylation). Thought to be involved in arabinan metabolism, phosphorylating perhaps EMBR|Rv1267c [catalytic activity: ATP + a protein = ADP + a phosphoprotein]. Probable transmembrane serine/threonine-protein kinase H PknH (protein kinase H) (STPK H) Rv1266c, (MTCY50.16), len: 626 aa. Probable pknH, transmembrane serine/threonine-protein kinase (see citation below), similar to many e.g. PKN1_MYXXA|P33973 pkn1 (693 aa), FASTA scores: opt: 611, E(): 1.4e- 14, (29.7% identity in 492 aa overlap); etc. Contains PS00107 Protein kinases ATP-binding region signature; PS00108 Serine/Threonine protein kinases active-site signature. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases. Experimental studies show evidence of auto-phosphorylation. P9WI71 No regulatory proteins Q11053 P9WI71 GO:0006468,GO:0004674,GO:0005524 2.7.11.1 P9WI71 NC_000962.3 Mycobrowser_v5 CDS 1424197 1424754 . + 0 Rv1274 lprB Unknown Possible lipoprotein LprB Rv1274, (MTCY50.08c), len: 185 aa. Possible lprB, lipoprotein; contains possible N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein lipid attachment site (PS00013). Some similarity to Rv1275. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WK53 No cell wall and cell processes P9WK53 GO:0005886 NC_000962.3 Mycobrowser_v5 CDS 1424751 1425293 . + 0 Rv1275 lprC Unknown Possible lipoprotein LprC Rv1275, (MTCY50.07c), len: 180 aa. Possible lprC, lipoprotein; contains possible N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein lipid attachment site (PS00013). Some similarity to Rv1274. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O86337 No cell wall and cell processes O86337 NC_000962.3 Mycobrowser_v5 CDS 1425438 1425914 . - 0 Rv1276c Rv1276c Function unknown Conserved hypothetical protein Rv1276c, (MTCY50.06), len: 158 aa. Conserved hypothetical protein, similar to AL096844|SCI28.03 hypothetical protein from Streptomyces coelicolor (172 aa), FASTA scores: opt: 385, E(): 3.3e-19, (43.5% identity in 161 aa overlap). Some similarity to P76502|SIXA_ECOLI phosphohistidine phosphatase SIXA (161 aa), FASTA scores: opt: 146, E(): 0.0047, (31.9% identity in 116 aa overlap). Belongs to the SixA family of phosphatases. P9WGF9 No conserved hypotheticals Q11043 P9WGF9 GO:0016787 3.1.3.- P9WGF9 NC_000962.3 Mycobrowser_v5 CDS 1427414 1430041 . + 0 Rv1278 Rv1278 Unknown Hypothetical protein Rv1278, (MTCY50.04c), len: 875 aa. Hypothetical unknown protein, possible coiled-coil regions, contains PS00017 ATP/GTP-binding site motif A. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WM41 No conserved hypotheticals P9WM41 P9WM41 NC_000962.3 Mycobrowser_v5 CDS 1430062 1431648 . + 0 Rv1279 Rv1279 Function unknown; probably involved in cellular metabolism, probably electron-transfer-linked. Probable dehydrogenase FAD flavoprotein GMC oxidoreductase Rv1279, (MTCY50.03c), len: 528 aa. Probable dehydrogenase, FAD flavoprotein GMC oxidoreductase, similar to several e.g. dBETA_ECOLI|P17444 choline dehydrogenase from Escherichia coli (556 aa), FASTA scores, opt: 1047, E(): 0, (37.7% identity in 541 aa overlap). Similar to Rv0697 putative Mycobacterium tuberculosis GMC oxidoreductase. Contains PS00623 GMC oxidoreductases signature 1, and PS00624 GMC oxidoreductases signature 2. Belongs to the GMC oxidoreductases family. P9WMV5 No intermediary metabolism and respiration P64263 P9WMV5 GO:0006066,GO:0055114,GO:0016614,GO:0050660 1.1.-.- P9WMV5 NC_000962.3 Mycobrowser_v5 CDS 1445499 1445834 . - 0 Rv1291c Rv1291c Unknown Conserved hypothetical secreted protein Rv1291c, (MTCY373.10c), len: 111 aa. Conserved hypothetical secreted protein, similar to others in Mycobacterium tuberculosis e.g. Rv1271c|Q11048|YC71_MYCTU hypothetical 11.6 kDa protein (113 aa), FASTA score: opt: 246, E(): 1.7e-09, (40.0% identity in 110 aa overlap); Rv1804c, Rv1810, Rv0622, etc. P9WM33 No cell wall and cell processes P0A5E5 P9WM33 NC_000962.3 Mycobrowser_v5 CDS 1433433 1435271 . - 0 Rv1281c oppD Involved in active transport of oligopeptide across the membrane (import). Responsible for energy coupling to the transport system. Probable oligopeptide-transport ATP-binding protein ABC transporter OppD Rv1281c, (MTCY50.01), len: 612 aa. Probable oppD, oligopeptide-transport ATP-binding protein ABC transporter (see citation below), similar to others e.g. DPPD_BACSU|P26905 dipeptide transport ATP-binding protein from Bacillus subtilis (335 aa), FASTA scores: opt: 983, E(): 0, (48.6% identity in 319 aa overlap); etc. Contains 2 x PS00017 ATP/GTP-binding site motif A (P-loop); 2 x PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). P9WQJ5 No cell wall and cell processes P63395 P9WQJ5 GO:0016887,GO:0015833,GO:0015197,GO:0005524 P9WQJ5 NC_000962.3 Mycobrowser_v5 CDS 1435268 1436143 . - 0 Rv1282c oppC Involved in active transport of oligopeptide across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable oligopeptide-transport integral membrane protein ABC transporter OppC Rv1282c, (MTCY373.01c-MTCY3H3.01), len: 291 aa. Probable oppC, oligopeptide-transport integral membrane protein ABC transporter (see Braibant et al., 2000), similar to other integral membrane proteins e.g. OPPC_ECOLI|P77664 oligopeptide transport system permease from Escherichia coli (302 aa), FASTA scores: E(): 4.6e-33, (40.7% identity in 275 aa overlap); etc. Also similar to Rv3664c|DPPC probable peptide-transport integral membrane protein from Mycobacterium tuberculosis. P9WFZ9 No cell wall and cell processes P66964 P9WFZ9 GO:0015833,GO:0005886,GO:0015031,GO:0005215,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1436140 1437117 . - 0 Rv1283c oppB Involved in active transport of oligopeptide across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable oligopeptide-transport integral membrane protein ABC transporter OppB Rv1283c, (MTCY373.02c), len: 325 aa. Probable oppB, oligopeptide-transport integral membrane protein ABC transporter (see citation below), similar to other integral membrane proteins e.g. DPPB_ECOLI|P37316 dipeptide transport system permease protein from Escherichia coli (339 aa), FASTA scores: opt: 402, E(): 3.4e-20, (31.0% identity in 345 aa overlap); etc. Also similar to Rv3665c|DppB probable peptide-transport integral membrane protein from Mycobacterium tuberculosis. Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature. P9WFZ7 No cell wall and cell processes P66966 P9WFZ7 GO:0015833,GO:0005886,GO:0015031,GO:0005215,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1446379 1448031 . + 0 Rv1292 argS Involved in translation mechanism [catalytic activity: ATP + L-arginine + tRNA(ARG) = AMP + diphosphate + L-arginyl-tRNA(ARG)]. Probable arginyl-tRNA synthetase ArgS (ARGRS) (arginine--tRNA ligase) Rv1292, (MTCY373.12), len: 550 aa. Probable argS, Arginyl-tRNA synthetase, highly similar to SYR_MYCLE|P45840 Mycobacterium leprae (550 aa), FASTA scores: opt: 3115, E(): 0, (84.9% identity in 550 aa overlap). Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Belongs to class-I aminoacyl-tRNA synthetase family. P9WFW5 No information pathways P67569 P9WFW5 GO:0004814,GO:0006420,GO:0005737,GO:0005524 6.1.1.19 P9WFW5 NC_000962.3 Mycobrowser_v5 CDS 1508968 1509288 . + 0 Rv1344 mbtL Thought to be involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins Acyl carrier protein (ACP) MbtL Rv1344, (MTCY02B10.08), len: 106 aa. mbtL, acyl carrier protein, similar to others e.g. ACP_RHIME|P19372 Rhizobium meliloti (77 aa), FASTA scores: opt: 117, E(): 0.03, (29.9% identity in 67 aa overlap) and ACP_SYNY3|P20804 acyl carrier protein (acp) from Synechocystis sp (77 aa), FASTA scores: E(): 7.1e-05, (34.8% identity in 66 aa overlap). Also similar to Rv2244 and Rv0033 from Mycobacterium tuberculosis. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WQF1 No lipid metabolism P63452 P9WQF1 GO:0048037,GO:0005737,GO:0006826,GO:0000036 P9WQF1 NC_000962.3 Mycobrowser_v5 CDS 1438907 1440751 . + 0 Rv1286 cysN ATP sulfurylase may be the GTPase, regulating ATP sulfurylase activity [catalytic activity 1: ATP + sulfate = diphosphate + adenylylsulfate] and APS kinase catalyzes the synthesis of activated sulfate [catalytic activity 2: ATP + adenylylsulfate = ADP + 3'- phosphoadenylylsulfate]. First and second steps in the sulfate activation pathway. These reactions occurs early in the reductive branch of the cysteine biosynthetic pathway. Probable bifunctional enzyme CysN/CysC: sulfate adenyltransferase (subunit 1) + adenylylsulfate kinase Rv1286, (MTCY373.05), len: 614 aa. Probable cysN/cysC bifunctional enzyme, sulfate adenylyltransferase subunit 1 and Adenylylsulfate kinase (see Wooff et al., 2002), similar to CYSN_ECOLI|P23845 sulfate adenylate transferase subunit 1 from Escherichia coli (475 aa), FASTA scores: opt: 1291, E():0, (50.2% identity in 428 aa overlap). Contains 2 x PS00017 ATP/GTP-binding site motif A, PS00301 GTP-binding elongation factors signature. P9WNM5 No intermediary metabolism and respiration Q10600 P9WNM5 GO:0005525,GO:0003924,GO:0004020,GO:0004781,GO:0000103,GO:0005524 2.7.1.25,2.7.7.4 P9WNM5 NC_000962.3 Mycobrowser_v5 CDS 1440805 1441290 . + 0 Rv1287 Rv1287 Function unknown Conserved hypothetical protein Rv1287, (MTCY373.06), len: 161 aa. Conserved hypothetical protein, similar to VjeB family of proteins e.g. FASTA score: P44675|Y379_HAEIN hypothetical protein HI0379 (150 aa), FASTA scores: opt: 213, E(): 2.5e-08, (30.0% identity in 130 aa overlap) and YJEB_ECOLI|P21498 hypothetical 15.6 kDa protein in pura-vacb (141 aa), opt: 167, E(): 9.5e-06, (25.0% identity in 136 aa overlap). Belongs to the UPF0074 (RFF2) family. P9WME3 No conserved hypotheticals P67159 P9WME3 GO:0003677 P9WME3 NC_000962.3 Mycobrowser_v5 CDS 1441348 1442718 . + 0 Rv1288 Rv1288 Function unknown Conserved protein Rv1288, (MTCY373.07), len: 456 aa. Conserved protein, some similarity to A85B_MYCTU|P31952 antigen 85-b precursor (85b) (325 aa), FASTA scores: opt: 199, E(): 2.7e-06, (24.7% identity in 279 aa overlap). Also similar to Q01377|CSP1_CORGL PS1 protein precursor (related to antigen 85 complex) from Corynebacterium glutamicum (657 aa), FASTA scores: opt: 280, E(): 1.9e-10, (26.4% identity in 352 aa overlap). Seems to contain 3 LYSM repeats P9WM39 No conserved hypotheticals Q10614 P9WM39 GO:0016998 P9WM39 NC_000962.3 Mycobrowser_v5 CDS 1442767 1443399 . + 0 Rv1289 Rv1289 Unknown Unknown protein Rv1289, (MTCY373.08), len: 210 aa. Unknown protein. P9WM37 No conserved hypotheticals P9WM37 P9WM37 NC_000962.3 Mycobrowser_v5 CDS 1443482 1445047 . - 0 Rv1290c Rv1290c Function unknown. Thought to be involved in virulence. Conserved protein Rv1290c, (MTCY373.09c), len: 521 aa. Conserved protein (see citation below), similar to AL031013|SC8A6.09 hypothetical protein from Streptomyces coelicolor (443 aa), FASTA scores: opt: 371, E(): 9.5e-17, (28.3% identity in 446 aa overlap). P9WM35 No conserved hypotheticals P0A5E3 P9WM35 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1448028 1449371 . + 0 Rv1293 lysA Involved in biosynthesis of lysine (last step) [catalytic activity: MESO-2,6-diaminoheptanedioate = L-lysine + CO(2)]. Diaminopimelate decarboxylase LysA (DAP decarboxylase) Rv1293, (MTCY373.13), len: 447 aa. lysA, diaminopimelate decarboxylase (see citation below), almost identical to DCDA_MYCTU|P31848. Contains PS00878 Orn/DAP/Arg decarboxylases family 2 pyridoxal-P attachment site, PS00879 Orn/DAP/Arg decarboxylases family 2 signature 2. Belongs to family 2 of ornithine, DAP, and arginine decarboxylases. P9WIU7 No intermediary metabolism and respiration 1HKV,1HKW,2O0T P0A5M4 P9WIU7 GO:0009089,GO:0008836 4.1.1.20 P9WIU7 NC_000962.3 Mycobrowser_v5 CDS 1450697 1451779 . + 0 Rv1295 thrC Involved in threonine biosynthesis [catalytic activity: O-phospho-L-homoserine + H(2)O = L-threonine + phosphate] Threonine synthase ThrC (ts) Rv1295, (MTCY373.15), len: 360 aa. thrC, threonine synthase (see Parish et al., 1999), highly similar to THRC_MYCLE|P45837 Mycobacterium leprae (360 aa), FASTA scores: opt: 2202, E(): 0, (93.9% identity in 359 aa overlap). Contains PS00165 Serine/threonine dehydratases pyridoxal-phosphate attachment site. P9WG59 No intermediary metabolism and respiration 2D1F P66902 P9WG59 GO:0009088,GO:0004795,GO:0030170 4.2.3.1 P9WG59 NC_000962.3 Mycobrowser_v5 CDS 1451997 1452947 . + 0 Rv1296 thrB Threonine biosynthesis from asparate (fourth step) [catalytic activity: ATP + L-homoserine = ADP + O-phospho-L-homoserine]. Probable homoserine kinase ThrB Rv1296, (MTCY373.16), len: 316 aa. Probable thrB, homoserine kinase (see citation below), highly similar to KHSE_MYCLE|P45836 from Mycobacterium leprae (314 aa), FASTA scores, opt: 1657, E(): 0, (82.0% identity in 311 aa overlap). Contains PS00639 Eukaryotic thiol (cysteine) proteases histidine active site, and PS00627 GHMP kinases putative ATP-binding domain. P9WKE7 No intermediary metabolism and respiration P65224 P9WKE7 GO:0005737,GO:0004413,GO:0016310,GO:0009088,GO:0005524 2.7.1.39 P9WKE7 NC_000962.3 Mycobrowser_v5 CDS 1455163 1455405 . + 0 Rv1298 rpmE Involved in translation 50S ribosomal protein L31 RpmE Rv1298, (MTCY373.18), len: 80 aa. rpmE, 50s ribosomal protein L31, highly similar to many e.g. RL31_MYCLE|P45834 50s ribosomal protein L31 from Mycobacterium leprae (84 aa), FASTA scores: opt: 490, E(): 5.5e-28, (89.6% identity in 77 aa overlap). Contains PS01143 Ribosomal protein L31 signature. Belongs to the L31P family of ribosomal proteins. P9WHA1 No information pathways P66187 P9WHA1 GO:0019843,GO:0005840,GO:0003735,GO:0006412,GO:0046872 P9WHA1 NC_000962.3 Mycobrowser_v5 CDS 1455495 1456568 . + 0 Rv1299 prfA Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA Probable peptide chain release factor 1 PrfA (RF-1) Rv1299, (MTCY373.19), len: 357 aa. Probable prfA, peptide chain release factor 1 (rf-1), highly similar to many e.g. RF1_MYCLE|P45833 peptide chain release factor 1 (rf-1) from Mycobacterium leprae (357 aa), FASTA scores: opt: 2047, E(): 0, (89.3% identity in 356 aa overlap); also similar to Mycobacterium tuberculosis Rv3105c, prfB peptide chain release factor 2. Contains PS00745 Prokaryotic-type class I peptide chain release factors signature. Belongs to the prokaryotic and mitochondrial release factors family. P9WHG3 No information pathways P66016 P9WHG3 GO:0016149,GO:0006415,GO:0005737 P9WHG3 NC_000962.3 Mycobrowser_v5 CDS 2260665 2261144 . + 0 Rv2013 Rv2013 Required for the transposition of an insertion element. Transposase Rv2013, (MTCY39.04c), len: 159 aa. Transposase, shows similarity to N-terminal part of transposase and insertion element hypothetical proteins. Length changed since first submission (no clear start apparent). Q10844 No insertion seqs and phages Q10844 Q10844 GO:0004803,GO:0006313,GO:0003677 Q10844 NC_000962.3 Mycobrowser_v5 CDS 1459766 1460251 . + 0 Rv1303 Rv1303 Function unknown Conserved hypothetical transmembrane protein Rv1303, (MTCY373.23), len: 161 aa. Conserved hypothetical transmembrane protein, highly similar to P53431|Y02N_MYCLE hypothetical Mycobacterium leprae protein (153 aa), FASTA score: opt: 636, E():0, (69.8% identity in 149 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WM31 No cell wall and cell processes P64801 P9WM31 GO:0015078,GO:0016021,GO:0005886,GO:0045263,GO:0015986 P9WM31 NC_000962.3 Mycobrowser_v5 CDS 1467688 1468131 . + 0 Rv1312 Rv1312 Unknown Conserved hypothetical secreted protein Rv1312, (MTCY373.32), len: 147 aa. Conserved hypothetical secreted protein with potential N-terminal signal sequence. Highly similar to P53432|Y02W_MYCLE hypothetical Mycobacterium leprae protein (147 aa), FASTA score: opt: 884, E(): 0, (88.4% identity in 147 aa overlap). N-terminus hydrophobic. P9WM29 No cell wall and cell processes Q10620 P9WM29 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1460244 1460996 . + 0 Rv1304 atpB Key component of the proton channel; it may play a direct role in the translocation of protons (H+) across the membrane Probable ATP synthase a chain AtpB (protein 6) Rv1304, (MTCY373.24), len: 250 aa. Probable atpB, ATP synthase a chain, highly similar to ATP6_MYCLE|P45829 Mycobacterium leprae (251 aa), FASTA scores: opt: 1382, E(): 0, (84.0% identity in 250 aa overlap). Contains PS00449 ATP synthase a subunit signature. subunit: F-type ATPases have 2 components, cf(1) - the catalytic core - and cf(0) - the membrane proton channel. cf(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). cf(0) has three main subunits: A, B and C. Belongs to the ATPase a chain family. P9WPV7 No intermediary metabolism and respiration P63654 P9WPV7 GO:0016021,GO:0005886,GO:0042777,GO:0045263,GO:0046933 P9WPV7 NC_000962.3 Mycobrowser_v5 CDS 1461045 1461290 . + 0 Rv1305 atpE This is one of the three chains of the nonenzymatic component (cf(0) subunit) of the ATPase complex. Probable ATP synthase C chain AtpE (lipid-binding protein) (dicyclohexylcarbodiimide-binding protein) Rv1305, (MTCY373.25), len: 81 aa. Probable atpE, ATP synthase C chain, highly similar to P45828|ATPL_MYCLE Mycobacterium leprae (92.6% identity in 81 aa overlap). Contains PS00605 ATP synthase C subunit signature. subunit: F-type ATPases have 2 components, cf(1) - the catalytic core - and cf(0) - the membrane proton channel. cf(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). cf(0) has three main subunits: A, B and C. Belongs to the ATPase C chain family. P9WPS1 No intermediary metabolism and respiration P63691 P9WPS1 GO:0046933,GO:0016021,GO:0008289,GO:0005886,GO:0045263,GO:0015986 P9WPS1 NC_000962.3 Mycobrowser_v5 CDS 1461321 1461836 . + 0 Rv1306 atpF This is one of the three chains of the nonenzymatic component (cf(0) subunit) of the ATPase complex. Probable ATP synthase B chain AtpF Rv1306, (MTCY373.26), len: 171 aa. Probable atpF, ATP synthase B chain, highly similar to ATPF_MYCLE P45827 (170 aa), FASTA scores, opt: 802, E(): 0, (79.5% identity in 171 aa overlap). subunit: F-type ATPases have 2 components, cf(1) - the catalytic core - and cf(0) - the membrane proton channel. cf(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). cf(0) has three main subunits: A, B and C. Belongs to the ATPase B chain family. P9WPV5 No intermediary metabolism and respiration P63656 P9WPV5 GO:0046933,GO:0016021,GO:0005886,GO:0045263,GO:0015986 P9WPV5 NC_000962.3 Mycobrowser_v5 CDS 1470321 1471577 . + 0 Rv1315 murA Involved in cell wall formation; peptidoglycan biosynthesis. Adds enolpyruvyl to UDP-N-acetylglucosamine [catalytic activity: phosphoenolpyruvate + UDP-N-acetyl-D- glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine] Probable UDP-N-acetylglucosamine 1-carboxyvinyltransferase MurA Rv1315, (MTCY373.35-MTCY149.01), len: 418 aa. Probable murA, UDP-N-acetylglucosamine 1-carboxyvinyltransferase (see Belanger & Inamine 2000), highly similar to many e.g. MURA_MYCLE|P45821 (418 aa), FASTA scores: opt: 2495, E(): 0, (96.2% identity in 396 aa overlap). Belongs to the EPSP synthase family. MURA subfamily. P9WJM1 No cell wall and cell processes P0A5L2 P9WJM1 GO:0008760,GO:0007049,GO:0051301,GO:0007047,GO:0005737,GO:0009252,GO:0008360,GO:0019277 2.5.1.7 P9WJM1 NC_000962.3 Mycobrowser_v5 CDS 1467315 1467680 . + 0 Rv1311 atpC Produces ATP from ADP in the presence of a proton gradient across the membrane [catalytic activity: ATP + H(2)O + H(+)(in) = ADP + phosphate + H(+)(out)] Probable ATP synthase epsilon chain AtpC Rv1311, (MTCY373.31), len: 121 aa. Probable atpC, ATP synthase epsilon chain, highly similar to ATPE_MYCLE|P45822 Mycobacterium leprae (124 aa), FASTA scores: opt: 682, E(): 5.4e-40, (87.6% identity in 121 aa overlap). subunit: F-type ATPases have 2 components, cf(1) - the catalytic core - and cf(0) - the membrane proton channel. cf(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). cf(0) has three main subunits: A, B and C. Belongs to the ATPase epsilon chain family. P9WPV1 No intermediary metabolism and respiration P63662 P9WPV1 GO:0046933,GO:0005886,GO:0042777,GO:0045261,GO:0046961,GO:0005524 P9WPV1 NC_000962.3 Mycobrowser_v5 CDS 1464884 1465801 . + 0 Rv1309 atpG Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the cf(0) complex. Probable ATP synthase gamma chain AtpG Rv1309, (MTCY373.29), len: 305 aa. Probable atpG, ATP synthase gamma chain, highly similar to ATPG_MYCLE|P45824 ATP synthase gamma chain from Mycobacterium leprae (298 aa), FASTA scores: opt: 1579, E():0, (83.9% identity in 305 aa overlap). Contains PS00153 ATP synthase gamma subunit signature. subunit: F-type ATPases have 2 components, cf(1) - the catalytic core - and cf(0) - the membrane proton channel. cf(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). cf(0) has three main subunits: A, B and C. Belongs to the ATPase gamma chain family. P9WPU9 No intermediary metabolism and respiration P63671 P9WPU9 GO:0015986,GO:0046933,GO:0005886,GO:0045261,GO:0046961,GO:0005524 P9WPU9 NC_000962.3 Mycobrowser_v5 CDS 1465841 1467301 . + 0 Rv1310 atpD Produces ATP from ADP in the presence of a proton gradient across the membrane. The beta chain is the catalytic subunit [catalytic activity: ATP + H(2)O + H(+)(in) = ADP + phosphate + H(+)(out)] Probable ATP synthase beta chain AtpD Rv1310, (MTCY373.30), len: 486 aa. Probable atpD, ATP synthase beta chain, highly similar to ATPB_MYCLE|P45823 Mycobacterium leprae (485 aa), FASTA score: opt: 2916, E(): 0, (92.6% identity in 484 aa overlap). Contains PS00017 ATP/GTP-binding site motif A, PS00152 ATP synthase alpha and beta subunits signature. subunit: F-type ATPases have 2 components, cf(1) - the catalytic core - and cf(0) - the membrane proton channel. cf(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). cf(0) has three main subunits: A, B and C. Belongs to the ATPase alpha/beta chains family. P9WPU5 No intermediary metabolism and respiration P63677 P9WPU5 GO:0046933,GO:0008553,GO:0005886,GO:0042777,GO:0045261,GO:0046961,GO:0005524 3.6.3.14 P9WPU5 NC_000962.3 Mycobrowser_v5 CDS 1500661 1500966 . + 0 Rv1331 Rv1331 Function unknown Conserved hypothetical protein Rv1331, (MTCY130.16), len: 101 aa. Conserved hypothetical protein, highly similar to U00014|ML014 B1549_C2_207 from Mycobacterium leprae (94 aa), FASTA scores: opt: 573, E(): 2.9e-40, (90.3% identity in 93 aa overlap). Similar to AL096852|SCE19A_16 hypothetical protein from Streptomyces coelicolor (105 aa), FASTA scores: opt: 377, E(): 2.9e-22, (60.0% identity in 105 aa overlap). P9WPC1 No conserved hypotheticals P67647 P9WPC1 GO:0006508,GO:0030163 P9WPC1 NC_000962.3 Mycobrowser_v5 CDS 1508184 1508546 . - 0 Rv1342c Rv1342c Unknown Conserved membrane protein Rv1342c, (MTCY02B10.06c), len: 120 aa. Conserved membrane protein. Highly similar to G466926|P54133 hypothetical protein B1549_F2_59 from Mycobacterium leprae (119 aa), FASTA scores, opt: 544, E(): 1.9e-29, (68.3 % identity in 120 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WM19 No cell wall and cell processes P9WM19 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2341808 2342944 . + 0 Rv2084 Rv2084 Unknown Hypothetical protein Rv2084, (MTCY49.23), len: 378 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLK1 No unknown P9WLK1 P9WLK1 NC_000962.3 Mycobrowser_v5 CDS 1469671 1470252 . - 0 Rv1314c Rv1314c Function unknown Conserved protein Rv1314c, (MTCY373.34c), len: 193 aa. Conserved protein, highly similar to P53523|Y02Y_MYCLE hypothetical Mycobacterium leprae protein (191 aa), FASTA score: opt:1019, E(): 0, (81.2% identity in 191 aa overlap). Some similarity with YDHW_CITFR|P45515 hypothetical 19.8 kDa protein in dhar-dhat intergenic region (176 aa), FASTA scores: opt: 297, E(): 1.6e-13, (37.6% identity in 178 aa overlap). Also similar to hypothetical protein AE002007|AE002007_3 Deinococcus radiodurans (185 aa), FASTA score: opt: 386, E(): 7.7e-19, (42.4% identity in 172 aa overlap). P9WP99 No conserved hypotheticals 2G2D P64803 P9WP99 GO:0008817,GO:0009236,GO:0005737,GO:0005524 2.5.1.17 P9WP99 NC_000962.3 Mycobrowser_v5 CDS 1477134 1477631 . - 0 Rv1316c ogt Repair of alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated [catalytic activity: DNA (containing 6-O-methylguanine) + [protein]-L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.] Methylated-DNA--protein-cysteine methyltransferase Ogt (6-O-methylguanine-DNA methyltransferase) (O-6-methylguanine-DNA-alkyltransferase) Rv1316c, (MTCY130.01c), len: 165 aa. Ogt, methylated-dna--protein-cysteine methytransferase (see citation below), similar to many e.g. OGT_HAEIN|P44687 Haemophilus influenzae (190 aa), FASTA scores: opt: 405, E(): 6.5e-20, (41.9% identity in 155 aa overlap). Contains PS00374 Methylated-DNA--protein-cysteine methyltransferase active site. P9WJW5 No information pathways P0A696 P9WJW5 GO:0003908,GO:0006281 2.1.1.63 P9WJW5 NC_000962.3 Mycobrowser_v5 CDS 1479199 1480824 . - 0 Rv1318c Rv1318c Thought to play an essential role in regulation of cellular metabolism by catalysing the synthesis of a second messenger, camp [catalytic activity: ATP = 3',5'-cyclic AMP + pyrophosphate]. Possible adenylate cyclase (ATP pyrophosphate-lyase) (adenylyl cyclase) Rv1318c, (MTCY130.03c), len: 541 aa. Possible adenylate cyclase. Some similarity at the c-terminus to CYAA_RHIME|P19485 adenylate cyclase from Rhizobium meliloti (193 aa), FASTA scores, opt: 270, E(): 2.5e-11, (28.8% identity in 184 aa overlap); similar to other mycbacterium tuberculosis putative adenylate cyclases e.g. Rv1319c|MTCY130.04c (535 aa), FASTA scores: opt: 2505, E(): 0, (71.0% identity in 534 aa overlap), also similar to Rv1320c|MTCY130.05c (567 aa), FASTA scores, opt: 2423, E(): 0, (68.7% identity in 534 aa overlap). N-terminus is hydrophobic. Belongs to adenylyl cyclase class-3 family. P9WQ33 No intermediary metabolism and respiration P63527 P9WQ33 GO:0016021,GO:0016849,GO:0005886,GO:0004871,GO:0007165,GO:0009190 P9WQ33 NC_000962.3 Mycobrowser_v5 CDS 1500926 1501582 . + 0 Rv1332 Rv1332 Involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv1332, (MTCY130.17), len: 218 aa. Possible regulatory protein, high similarity to ML014|U00014 M. leprae B1549_C3_236 (222 aa), FASTA scores: opt: 1158, E(): 0, (75.6% identity in 221 aa overlap). Helix turn helix motif fram aa 8-29 (+3.03 SD). P9WM25 No regulatory proteins P64809 P9WM25 NC_000962.3 Mycobrowser_v5 CDS 1497195 1499189 . - 0 Rv1329c dinG Probable helicase involved in DNA repair and perhaps also replication. Probable ATP-dependent helicase DinG Rv1329c, (MTCY130.14c), len: 664 aa. Probable dinG, ATP-dependent helicase (see citation below), similar to several e.g. DING_HAEIN|P44680 probable ATP-dependent helicase ding from Haemophilus influenzae (640 aa), FASTA scores: opt: 685, E(): 2.3e-38, (32.8% identity in 644 aa overlap). Contains PS00017 ATP/GTP-binding site motif A. P9WMR5 No information pathways P64314 P9WMR5 GO:0008026,GO:0003677,GO:0006310,GO:0006281,GO:0005524 3.6.4.12 P9WMR5 NC_000962.3 Mycobrowser_v5 CDS 1482514 1484217 . - 0 Rv1320c Rv1320c Thought to play an essential role in regulation of cellular metabolism by catalysing the synthesis of a second messenger, cAMP. May be involved in virulence [catalytic activity: ATP = 3',5'-cyclic AMP + pyrophosphate]. Possible adenylate cyclase (ATP pyrophosphate-lyase) (adenylyl cyclase) Rv1320c, (MTCY130.05c), len: 567 aa. Possible adenylate cyclase (see Rindi et al., 1999). Some similarity at the C-terminus to CYAA_RHIME|P19485 adenylate cyclase from Rhizobium meliloti (193 aa), FASTA scores: opt: 277, E(): 2e-12, (34.0% identity in 156 aa overlap); similar to other mycbacterium tuberculosis putative adenylate cyclases e.g. Rv1318c|MTCY130.03c (541 aa), FASTA scores: opt: 2423, E(): 0, (68.7% identity in 534 aa overlap); Rv1319c|MTCY130.04c (535 aa), FASTA scores: opt: 2354, E(): 0, (66.3% identity in 534 aa overlap). N-terminus is hydrophobic. Belongs to adenylyl cyclase class-3 family. P9WQ29 No intermediary metabolism and respiration Q10633 P9WQ29 GO:0016021,GO:0016849,GO:0005886,GO:0004871,GO:0007165,GO:0009190 P9WQ29 NC_000962.3 Mycobrowser_v5 CDS 1485862 1487031 . + 0 Rv1323 fadA4 Function unknown, but supposed involvement in lipid degradation [catalytic activity: 2 acetyl-CoA = CoA + acetoacetyl-CoA]. Probable acetyl-CoA acetyltransferase FadA4 (acetoacetyl-CoA thiolase) Rv1323, (MTCY130.08), len: 389 aa. Probable fadA4, acetyl-CoA acetyltransferase, equivalent to THIL_MYCLE|P46707 possible acetyl-CoA C-acetyltransferase from Mycobacterium leprae (393 aa), FASTA scores: opt: 2218, E(): 0, (87.0% identity in 392 aa overlap). Also highly similar to others e.g. CAB70629.1|AL137242 probable acetoacetyl-CoA thiolase from Streptomyces coelicolor (401 aa); T51772 acetyl-CoA C-acetyltransferase [validated] from Alcaligenes latus (392 aa); etc. Some homologies indicate ATA start codon. Contains PS00098 Thiolases acyl-enzyme intermediate signature, PS00737 Thiolases signature 2, and PS00099 Thiolases active site. Belongs to the thiolase family. P9WG69 No lipid metabolism P66926 P9WG69 GO:0008152,GO:0003985 2.3.1.9 P9WG69 NC_000962.3 Mycobrowser_v5 CDS 1487161 1488075 . + 0 Rv1324 Rv1324 Thioredoxin participates in various redox reactions through the reversible oxidation of its active center dithiol, to a disulfide, & catalyzes dithiol-disulfide exchange reactions Possible thioredoxin Rv1324, (MTCY130.09), len: 304 aa. Possible thioredoxin, similar to several e.g. U00014|Q49716 TRXA from Mycobacterium leprae (255 aa), FASTA scores: opt: 1014, E(): 0, (69.7% identity in 228 aa overlap); THIO_RHOSH|P08058 TrxA from Rhodobacter sphaeroides (105 aa), FASTA scores: opt 196, E(): 1.9e-06, (33.0% identity in 103 aa overlap). Contains PS00339 Aminoacyl-transfer RNA synthetases class-II signature 2. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WG61 No intermediary metabolism and respiration P64807 P9WG61 GO:0045454 P9WG61 NC_000962.3 Mycobrowser_v5 CDS 1518231 1518560 . + 0 Rv1351 Rv1351 Unknown Hypothetical protein Rv1351, (MTCY02B10.15), len: 109 aa. Hypothetical unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WM17 No conserved hypotheticals P9WM17 NC_000962.3 Mycobrowser_v5 CDS 1490117 1492312 . - 0 Rv1326c glgB Involved in glycogen biosynthesis (cytoplasmic polysaccharides) (third step) [catalytic activity: formation of 1,6-glucosidic linkages of glycogen]. 1,4-alpha-glucan branching enzyme GlgB (glycogen branching enzyme) Rv1326c, (MTCY130.11c), len: 731 aa. glgB, 1,4-alpha-glucan branching enzyme, similar to others e.g. GLGB_ECOLI|P07762 Escherichia coli (728 aa), FASTA scores: opt: 2330, E(): 0, (48.7% identity in 719 aa overlap). Similar to other Mycobacterium tuberculosis putative alpha-glucan branching enzymes Rv1562c, Rv1563c. Belongs to family 13 of glycosyl hydrolases, also known as the alpha-amylase family. P9WN45 No intermediary metabolism and respiration 3K1D Q10625 P9WN45 GO:0043169,GO:0005978,GO:0004553,GO:0003844 2.4.1.18 P9WN45 NC_000962.3 Mycobrowser_v5 CDS 1494564 1497155 . + 0 Rv1328 glgP Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties [catalytic activity: {(1,4)-alpha-D-glucosyl}(N) + phosphate = {(1,4)-alpha-D-glucosyl}(N-1) + alpha-D-glucose 1-phosphate]. Probable glycogen phosphorylase GlgP Rv1328, (MTCY130.13), len: 863 aa. Probable glgP, glycogen phosphorylase, similar to many e.g. PHSG_HAEIN|P45180 glycogen phosphorylase from Haemophilus influenzae (821 aa), FASTA scores: E(): 6.9e-08, (25.6% identity in 675 aa overlap). Belongs to the glycogen phosphorylase family. P9WMW1 No intermediary metabolism and respiration Q10639 P9WMW1 GO:0004645,GO:0030170,GO:0005977 2.4.1.1 P9WMW1 NC_000962.3 Mycobrowser_v5 CDS 1503394 1504365 . + 0 Rv1336 cysM Involved in cysteine biosynthesis [catalytic activity: O-phospho-L-serine + H(2)S = L-cysteine + phosphate] Cysteine synthase B CysM (CSASE B) (O-phosphoserine sulfhydrylase B) (O-phosphoserine (thiol)-lyase B) Rv1336, (MTCY130.21), len: 323 aa. cysM, cysteine synthase B, similar to many e.g. CYSM_ECOLI|P16703 Escherichia coli (303 aa), FASTA scores: opt: 720, E(): 4.6e-40, (41.1% identity in 302 aa overlap). Also similar to other Mycobacterium tuberculosis cysteine synthase subunits e.g. Rv1077, Rv2334, Rv0848, etc. Contains PS00901 Cysteine synthase/cystathionine beta-synthase P-phosphate attachment site. Belongs to the cysteine synthase/cystathionine beta-synthase family. P9WP53 No intermediary metabolism and respiration 3DKI,3DWG,3DWI,3FGP P63873 P9WP53 GO:0004124,GO:0030170,GO:0016740,GO:0006535 2.5.1.47 P9WP53 NC_000962.3 Mycobrowser_v5 CDS 1518763 1519134 . + 0 Rv1352 Rv1352 Function unknown Conserved protein Rv1352, (MTCY02B10.16), len: 123 aa. Conserved protein, some similarity to Rv1906c|MTCY180.12 hypothetical protein from Mycobacterium tuberculosis (156 aa), FASTA scores: E(): 4e-05, (36.2% identity in 116 aa overlap). P9WM15 No conserved hypotheticals P9WM15 P9WM15 NC_000962.3 Mycobrowser_v5 CDS 2358033 2358206 . - 0 Rv2099c PE21 Function unknown PE family protein PE21 Rv2099c, (MTCY49.39c), len: 58 aa. PE21, Member of the Mycobacterium tuberculosis PE family (see Brennan and Delogu, 2002); 5'-end of Rv2098c|PE_PGRS36|MTCY49.38c, then frameshifts. Sequence has been checked, no errors found. Q79FH7 Yes PE/PPE Q79FH7 Q79FH7 NC_000962.3 Mycobrowser_v5 CDS 1501599 1502633 . + 0 Rv1333 Rv1333 Function unknown; probably involved in cellular metabolism Probable hydrolase Rv1333, (MTCY130.18), len: 344 aa. Possible hydrolase, similar to Q57326|D26094 endo-type 6-aminohexanoate oligomer hydrolase (355 aa), fasta scores: E(): 1.4e-10, (31.9% identity in 339 aa overlap). Equivalent to P53425|YD33_MYCLE hypothetical 36.1 KD protein B154 Mycobacterium leprae (362 aa), FASTA scores: opt: 1735, E(): 0, (76.7% identity in 352 aa overlap). P9WM23 No intermediary metabolism and respiration P64811 P9WM23 GO:0005886,GO:0016021 P9WM23 NC_000962.3 Mycobrowser_v5 CDS 1502641 1503081 . + 0 Rv1334 mec Possibly involved in cysteine biosynthesis. Generates cysteine from hydrolysis of CysO-CYS. Possible hydrolase Rv1334, (MTCY130.19), len: 146 aa. Possible mec, hydrolase (See Burns et al., 2005), similar to AL096852|SCE19A_13 hypothetical protein from Streptomyces coelicolor (140 aa), Fasta scores: opt: 579, E(): 0, (65.0% identity in 140 aa overlap); and Q54330|M29166 MEC+ from Streptomyces kasugaensis (115 aa), FASTA scores; E(): 7.6e-33, (56.9% identity in 109 aa overlap). P9WHS1 No intermediary metabolism and respiration P64813 P9WHS1 P9WHS1 NC_000962.3 Mycobrowser_v5 CDS 1503103 1503384 . + 0 Rv1335 cysO Probably involved in cysteine biosynthesis, as sulfur donor in thiocarboxylated form. Sulfur carrier protein CysO Rv1335, (MT1376.1, MTCY130.20), len: 93 aa. CysO, sulfur carrier protein (See Burns et al., 2005). Note that previously known as cfp10A. Similar to hypothetical proteins from other organisms e.g. P74060|D90911 Synechocystis (109 aa), FASTA scores: E(): 2.3e-20, (49.5% identity in 93 aa overlap). P9WP33 No intermediary metabolism and respiration 3DWG,3DWM P0A646 P9WP33 GO:0006790 P9WP33 NC_000962.3 Mycobrowser_v5 CDS 1505075 1505890 . + 0 Rv1338 murI Involved in peptidoglycan biosynthesis. Provides the (R)-glutamic acid required for cell wall biosynthesis [catalytic activity: L-glutamate = D-glutamate] Probable glutamate racemase MurI Rv1338, (MTCY130.23), len: 271 aa. Probable murI, glutamate racemase, highly similar to many e.g. MURI_MYCLE|P46705 (272 aa), FASTA scores: opt: 1559, E(): 0, (88.9% identity in 271 aa overlap). Contains PS00924 Aspartate and glutamate racemases signature 2. P9WPW9 No cell wall and cell processes P63635 P9WPW9 GO:0008881,GO:0009252,GO:0008360,GO:0007047 5.1.1.3 P9WPW9 NC_000962.3 Mycobrowser_v5 CDS 1505917 1506738 . + 0 Rv1339 Rv1339 Function unknown Conserved protein Rv1339, (MTCY130.24), len: 273 aa. Conserved protein, highly similar to Y211_MYCLE|P50474 hypothetical protein b1549_c2_211 from Mycobacterium leprae (284 aa), FASTA scores: opt: 1672, E(): 0, (86.2% identity in 276 aa overlap). Also similar to AL096852|SCE19A.08 hypothetical protein from Streptomyces coelicolor (250 aa), FASTA scores: opt: 630, E(): 0, (42.2% identity in 256 aa overlap). Similar to M. tuberculosis hypothetical proteins Rv3796, Rv2407. Predicted to be an outer membrane protein (See Song et al., 2008). P9WGC1 No conserved hypotheticals P9WGC1 GO:0016787 P9WGC1 NC_000962.3 Mycobrowser_v5 CDS 1506755 1507534 . + 0 Rv1340 rphA RNase PH is a phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [catalytic activity: {tRNA}(N+1) + phosphate = {tRNA}(N) + a nucleoside diphosphate]. Probable ribonuclease RphA (RNase PH) (tRNA nucleotidyltransferase) Rv1340, (MTCY130.25), len: 259 aa. Probable rphA, Ribonuclease ph, highly similar to others e.g. RNPH_MYCLE|P37939 Mycobacterium leprae (259 aa), FASTA scores: opt: 1524, E(): 0, (88.8% identity in 259 aa overlap). Belongs to the RNASE PH family. P9WGZ7 No information pathways 3B4T Q10628 P9WGZ7 GO:0000049,GO:0009022,GO:0008033,GO:0004549,GO:0000175 2.7.7.56 P9WGZ7 NC_000962.3 Mycobrowser_v5 CDS 1539180 1539440 . + 0 Rv1366A Rv1366A Unknown Conserved protein Rv1366A, len: 86 aa. Conserved protein. V5QQR7 No conserved hypotheticals V5QQR7 NC_000962.3 Mycobrowser_v5 CDS 1507573 1508187 . + 0 Rv1341 Rv1341 Function unknown Conserved protein Rv1341, (MTCY130.26), len: 204 aa. Conserved protein, some similarity to P52061|YGGV_ECOLI hypothetical protein yggV (197 aa), FASTA scores: opt: 521, E(): 7.9e-27, (46.0% identity in 200 aa overlap). Equivalent to ML014|U00014 hypothetical protein B1549_C2_213 from Mycobacterium leprae (285 aa), FASTA scores: opt: 1073, E(): 0, (83.0% identity in 206 aa overlap). P9WMR7 No conserved hypotheticals P64307 P9WMR7 GO:0017111 3.6.1.15 P9WMR7 NC_000962.3 Mycobrowser_v5 CDS 1510846 1512006 . + 0 Rv1346 mbtN Thought to be involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins Acyl-CoA dehydrogenase MbtN Rv1346, (MTCY02B10.10), len: 386 aa. mbtN, acyl-CoA dehydrogenase, similar to many e.g. NP_251579.1|NC_002516 probable acyl-CoA dehydrogenase from Pseudomonas aeruginosa (386 aa); NP_036951.1|NM_012819|ACDL_RAT|P15650 acyl Coenzyme A dehydrogenase (long chain) from Rattus norvegicus (430 aa), FASTA scores: opt: 414, E(): 1.2e-18, (26.1% identity in 376 aa overlap); etc. Note that previously known as fadE14. P9WQF9 No lipid metabolism P63431 P9WQF9 GO:0006826,GO:0055114,GO:0003995 1.3.99.- P9WQF9 NC_000962.3 Mycobrowser_v5 CDS 1511973 1512605 . - 0 Rv1347c mbtK Involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins Lysine N-acetyltransferase MbtK Rv1347c, (MTCY02B10.11c), len: 210 aa. MbtK, lysine N-acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Some similarity to the C-terminus of malonyl-coenzyme A carboxylases e.g. G545170 malonyl-coenzyme A carboxylase (417 aa), FASTA scores: opt: 392, E(): 4.9 e-20, (35.6% identity in 174 aa overlap). P9WK15 No lipid metabolism 1YK3 P64819 P9WK15 GO:0006826,GO:0008415 2.3.1.- P9WK15 NC_000962.3 Mycobrowser_v5 CDS 1531348 1532370 . + 0 Rv1360 Rv1360 Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv1360, (MTCY02B10.24), len: 340 aa. Probable oxidoreductase. Similar to Q49598|G1002714 coenzyme F420-dependent n5, n10-methylenetetrahydromethanopterin reductase from Methanopyrus kandleri (349 aa), FASTA scores: opt: 264, E(): 4.4e-11, (26.3% identity in 323 aa overlap). P9WM03 No intermediary metabolism and respiration P64831 P9WM03 P9WM03 NC_000962.3 Mycobrowser_v5 CDS 1539512 1540645 . - 0 Rv1367c Rv1367c Function unknown (possibly involved in cell wall biosynthesis). Conserved protein Rv1367c, (MTCY02B12.01c,MTCY02B10.31c), len: 377 aa. Conserved protein. Some similarity to penicillin binding proteins e.g. PBPE_BACSU|P32959 penicillin-binding protein 4* (pbp 4*) from Bacillus subtilis (451 aa), FASTA scores: E(): 6.9e-06, (23.6% identity in 373 aa overlap). Similar to AL031107|SC5A7.06 hypothetical protein from Streptomyces coelicolor (409 aa), FASTA scores: opt: 675, E(): 0, (40.4% identity in 339 aa overlap). P9WLZ3 No conserved hypotheticals Q11037 P9WLZ3 P9WLZ3 NC_000962.3 Mycobrowser_v5 CDS 1541994 1542980 . - 0 Rv1369c Rv1369c Possibly required for the transposition of the insertion element IS6110. Probable transposase Rv1369c, (MTCY02B12.03c), len: 328 aa. Probable transposase subunit for IS6110. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv1368c and Rv1369c, the sequence UUUUAAAG (directly upstream of Rv1369c) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 34 aa). P9WKH9 No insertion seqs and phages P0C5G8 P9WKH9 GO:0015074,GO:0006310,GO:0032196,GO:0003677 P9WKH9 NC_000962.3 Mycobrowser_v5 CDS 1517491 1518234 . + 0 Rv1350 fabG2 Involved in the fatty acid biosynthesis pathway (first reduction step) [catalytic activity: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH]. Probable 3-oxoacyl-[acyl-carrier protein] reductase FabG2 (3-ketoacyl-acyl carrier protein reductase) Rv1350, (MTCY02B10.14), len: 247 aa. Probable fabG2, 3-oxoacyl-[acyl-carrier protein] reductase, highly similar to many e.g. NP_350157.1|NC_003030 3-ketoacyl-acyl carrier protein reductase from Clostridium acetobutylicum (249 aa); NP_229523.1|NC_000853 3-oxoacyl-(acyl carrier protein) reductase from Thermotoga maritima (246 aa); AAC44307.1|U59433 3-ketoacyl-acyl carrier protein reductase from Bacillus subtilis (246 aa); etc. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. P9WGR9 No lipid metabolism P66781 P9WGR9 GO:0055114,GO:0016491 1.-.-.- P9WGR9 NC_000962.3 Mycobrowser_v5 CDS 1519200 1519985 . - 0 Rv1353c Rv1353c Involved in transcriptional mechanism Probable transcriptional regulatory protein Rv1353c, (MTCY02B10.17c), len: 261 aa. Probable transcriptional regulatory protein, similar to TER1_ECOLI|P03038 tetracycline repressor protein class a from Escherichia coli (216 aa), FASTA scores, opt: 231, E(): 1.6e-08, (31.3% identity in 211 aa overlap). Helix turn helix motif present at aa 3859 (+3.59 SD). Belongs to the TetR/AcrR family of transcriptional regulators. P9WMD3 No regulatory proteins P67434 P9WMD3 GO:0006355,GO:0016566,GO:0006350,GO:0003700,GO:0016481 P9WMD3 NC_000962.3 Mycobrowser_v5 CDS 1521885 1524032 . - 0 Rv1355c moeY Involved in biosynthesis of a demolybdo cofactor (molybdopterin), necessary for molybdoenzymes. Plays a role in the activation of the small subunit of the molybdopterin converting factor (MOAD) Possible molybdopterin biosynthesis protein MoeY Rv1355c, (MTCY02B10.19c), len: 715 aa. Possible moeY, Molybdopterin biosynthesis protein, very weak similarity to MOEB_ECOLI|P12282 molybdopterin biosynthesis moeb protein (249 aa), FASTA scores, opt: 180, E(): 8.5e-05, (29.3% identity in 174 aa overlap). P9WM11 No intermediary metabolism and respiration Q11025 P9WM11 GO:0003824,GO:0008152 P9WM11 NC_000962.3 Mycobrowser_v5 CDS 1524029 1524820 . - 0 Rv1356c Rv1356c Unknown Hypothetical protein Rv1356c, (MTCY02B10.20c), len: 263 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WM09 No conserved hypotheticals P9WM09 NC_000962.3 Mycobrowser_v5 CDS 1525293 1526216 . - 0 Rv1357c Rv1357c Function unknown Conserved hypothetical protein Rv1357c, (MTCY02B10.21c), len: 307 aa. Conserved hypothetical protein, similar to members of the YEGE/YHJK/YJCC family e.g. Y4LL_RHISN|P55552 hypothetical protein Y4ll from Rhizobium sp. (827 aa), FASTA scores: E(): 0, (37.7% identity in 257 aa overlap), also similar to Rv1354c|MTCY02B10.18c (34.0% identity in 253 aa overlap). Belongs to the YEGE/YHDA/YHJK/YJCC family. P9WM07 No conserved hypotheticals P64829 P9WM07 GO:0005886,GO:0016021 P9WM07 NC_000962.3 Mycobrowser_v5 CDS 1532443 1533633 . - 0 Rv1361c PPE19 Function unknown PPE family protein PPE19 Rv1361c, (MTCY02B10.25c), len: 396 aa. PPE19 (alternate gene name: mtb39b). Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, highly similar to many e.g. Rv1196|MTCI364.08|PPE18, FASTA scores: E(): 0, (84.9% identity in 397 aa overlap); MTCY274.23c (42.3% identity in 416 aa overlap); etc. Contains PS00501 Signal peptidases I serine active site. Note that expression of Rv1361c was demonstrated in lysates by immunodetection (see Dillon et al., 1999). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WI25 No PE/PPE Q11031 P9WI25 P9WI25 NC_000962.3 Mycobrowser_v5 CDS 1533948 1534610 . - 0 Rv1362c Rv1362c Function unknown Possible membrane protein Rv1362c, (MTCY02B10.26c), len: 220 aa. Possible membrane protein, similar to Mycobacterium tuberculosis hypothetical proteins e.g. Rv1362c|MTCY02B10.27c (25.9% identity in 216 aa overlap), Rv0177, Rv1973, Rv1972, etc. P9WM01 No cell wall and cell processes P9WM01 GO:0016021 P9WM01 NC_000962.3 Mycobrowser_v5 CDS 1534607 1535392 . - 0 Rv1363c Rv1363c Function unknown Possible membrane protein Rv1363c, (MTCY02B10.27c), len: 261 aa. Possible membrane protein, similar to Mycobacterium tuberculosis hypothetical proteins Rv1362c|MTCY02B10.26c (25.9% identity in 216 aa overlap); Rv1972|MTV051.10 and Rv0177 etc. P9WLZ9 No cell wall and cell processes P9WLZ9 GO:0016021 P9WLZ9 NC_000962.3 Mycobrowser_v5 CDS 1535683 1537644 . - 0 Rv1364c Rv1364c May be involved in regulating SIGF|Rv3286c Possible sigma factor regulatory protein Rv1364c, (MTCY02B10.28c), len: 653 aa. Possible sigma factor regulatory protein, some similarity to RSBU_BACSU|P40399 sigma factor sibg regulation protein from Bacillus subtilis (335 aa), FASTA scores: opt: 224, E(): 2e-07, (25.8% identity in 244 aa overlap). Also known as mursiF. P9WLZ7 No information pathways 3K3C,3K3D,3KE6,3KX0 Q11034 P9WLZ7 GO:0004721,GO:0006355,GO:0007165,GO:0000155,GO:0000160,GO:0005524 P9WLZ7 NC_000962.3 Mycobrowser_v5 CDS 1537783 1538169 . - 0 Rv1365c rsfA Regulates negatively Rv3287c|RSBW|USFX. Regulated by redox potential. Anti-anti-sigma factor RsfA (anti-sigma factor antagonist) (regulator of sigma F A) Rv1365c, (MTCY02B10.29c), len: 128 aa. RsfA, anti-anti-sigma factor (see citation below), similar to other Mycobacterium tuberculosis proteins e.g. Rv2638|MTCY441.08 (148 aa), FASTA scores: E(): 0, (53.6% identity in 125 aa overlap); Rv1904, Rv3687c. Weak similarity to putative anti-anti-sigma factors e.g. AF134889|AF134889_1 Streptomyces coelicolor (113 aa), FASTA scores: opt: 137, E(): 0.004, (26.0% identity in 100 aa overlap). P9WGE3 No information pathways Q11035 P9WGE3 GO:0006355,GO:0045152 P9WGE3 NC_000962.3 Mycobrowser_v5 CDS 1538390 1539211 . + 0 Rv1366 Rv1366 Unknown Hypothetical protein Rv1366, (MTCY02B10.30), len: 273 aa. Hypothetical unknown protein. P9WLZ5 No conserved hypotheticals P64833 P9WLZ5 GO:0015969 P9WLZ5 NC_000962.3 Mycobrowser_v5 CDS 2009172 2009990 . + 0 Rv1775 Rv1775 Function unknown Conserved hypothetical protein Rv1775, (MTCY25C11.02), unknown, len: 272 aa. Conserved hypothetical protein, similar to O28806|AF1466 conserved hypothetical protein from Archaeoglobus fulgidus (255 aa), FASTA scores: opt: 364, E(): 1e-17, (29.2% identity in 267 aa overlap). O33178 No conserved hypotheticals O33178 O33178 NC_000962.3 Mycobrowser_v5 ncRNA 1547129 1547268 . + 0 MTB000076 MTS1082 Unknown Putative small regulatory RNA MTS1082, putative small regulatory RNA (See Arnvig et al., 2011), ends not mapped, ~150 bp band detected by Northern blot. No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 1547072 1547530 . - 0 Rv1374c Rv1374c Unknown Hypothetical protein Rv1374c, (MTCY02B12.08c), len: 152 aa. Hypothetical unknown protein. P71802 No conserved hypotheticals P71802 P71802 NC_000962.3 Mycobrowser_v5 CDS 1549148 1550641 . + 0 Rv1376 Rv1376 Function unknown Conserved hypothetical protein Rv1376, (MTCY02B12.10), len: 497 aa. Conserved hypothetical protein, some similarity to hypothetical proteins from several organisms e.g. Q52872|U39409 Rhizobium leguminosarum (247 aa), FASTA scores: E(): 2.1e-12, (34.7% identity in 219 aa overlap). P71804 No conserved hypotheticals P71804 P71804 P71804 NC_000962.3 Mycobrowser_v5 CDS 1542929 1543255 . - 0 Rv1370c Rv1370c Possibly required for the transposition of the insertion element IS6110. Putative transposase for insertion sequence element IS6110 (fragment) Rv1370c, (MTCY02B12.04c), len: 108 aa. Putative transposase for IS6110 (fragment), identical to many other Mycobacterium tuberculosis IS6110 transposase subunits e.g. Q50686|YIA4_MYCTU Insertion element IS6110 hypothetical 12.0 kDa protein (108 aa), fasta scores: E(): 1.4e-43, (100.00% identity in 108 aa overlap). The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv1368c and Rv1369c, the sequence UUUUAAAG (directly upstream of Rv1369c) maybe responsible for such a frameshifting event (see McAdam et al., 1990). P9WKH5 No insertion seqs and phages P0C5G9 P9WKH5 GO:0004803,GO:0006313,GO:0003677 P9WKH5 NC_000962.3 Mycobrowser_v5 CDS 1543359 1544828 . + 0 Rv1371 Rv1371 Unknown Probable conserved membrane protein Rv1371, (MTCY02B12.05), len: 489 aa. Probable membrane protein. Weak similarity to delta 5 fatty acid desaturases e.g. AB022097|AB022097_1 Dictyostelium discoideum (467 aa), FASTA score: opt: 173, E(): 0.00052, (22.4% identity in 438 aa overlap); and Homo sapiens. P71799 No cell wall and cell processes P71799 P71799 GO:0006629,GO:0055114,GO:0016491,GO:0020037 NC_000962.3 Mycobrowser_v5 CDS 1544825 1546006 . + 0 Rv1372 Rv1372 Function unknown Conserved hypothetical protein Rv1372, (MTCY02B12.06), len: 393 aa. Conserved hypothetical protein, similar to several chalcone synthases e.g. CHS2_GERHY|P48391 chalcone synthase 2 from gerbra hybrid (402 aa), FASTA scores: opt: 511, E(): 7e-26, (28.4% identity in 380 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical chalcone synthases, Rv1665, Rv1660. P9WPF1 No conserved hypotheticals 1TED,1TEE Q7D8I1 P9WPF1 GO:0009058,GO:0008415 P9WPF1 NC_000962.3 Mycobrowser_v5 CDS 1546012 1546992 . + 0 Rv1373 Rv1373 Involved in sulfation: activity towards typical ceramide glycolipids and trehalose glycolipids. Glycolipid sulfotransferase Rv1373, (MTCY02B12.07), len: 326 aa. Glycolipid sulfotransferase (see citation below); slight similarity to sulfotransferases e.g. SUOE_CAVPO|P49887 estrogen sulfotransferase from Cavia porcellus (Guinea pig) (296 aa), FASTA scores, opt: 165, E():0.00054, (24.5% identity in 294 aa overlap). P9WGB9 No intermediary metabolism and respiration P71801 P9WGB9 GO:0008146 2.8.2.- P9WGB9 NC_000962.3 Mycobrowser_v5 CDS 1695281 1695946 . - 0 Rv1505c Rv1505c Function unknown Conserved hypothetical protein Rv1505c, (MTCY277.27c), len: 221 aa. Conserved hypothetical protein, some similarity to hypothetical proteins and glycosylases e.g. P71063|O08181 hypothetical 22.5 kDa protein YVFD from Bacillus subtilis (216 aa), FASTA scores: E(): 2.4e-08, (25.5% identity in 196 aa overlap). P71784 No conserved hypotheticals P71784 GO:0016740 P71784 NC_000962.3 Mycobrowser_v5 CDS 2367359 2367655 . + 0 Rv2107 PE22 Function unknown PE family protein PE22 Rv2107, (MTCY261.03), len: 98 aa. PE22, Member of mycobacterial PE family (see citation below). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). L7N6B5 No PE/PPE Q7D7I5 L7N6B5 L7N6B5 NC_000962.3 Mycobrowser_v5 CDS 1574112 1574513 . - 0 Rv1397c vapC10 Unknown Possible toxin VapC10 Rv1397c, (MTCY21B4.14c), len: 133 aa. Possible vapC10, toxin, part of toxin-antitoxin (TA) operon with Rv1398c, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Conserved hypothetical protein, similar to Mycobacterium tuberculosis protein MTCY159.08C|Rv2548 (125 aa), FASTA scores: E(): 2.3e-14, (42.4% identity in 125 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WFA7 No virulence, detoxification, adaptation P71665 P9WFA7 P9WFA7 NC_000962.3 Mycobrowser_v5 CDS 1552654 1553235 . + 0 Rv1379 pyrR Binds to the conserved sequence in the PYR operon mRNA and disrupts the antiterminator, permitting terminator hairpin formation and promoting transcription termination Probable pyrimidine operon regulatory protein PyrR Rv1379, (MTCY02B12.13), len: 193 aa. Probable pyrR, pyrimidine operon regulatory protein, similar to PYRR_BACCL|P41007 pyrimidine operon regulatory protein from Bacillus caldolyticus (179 aa), FASTA scores: opt: 544, E(): 1.1e-30, (54.2% identity in 179 aa overlap). P9WHK3 No regulatory proteins 1W30 P65941 P9WHK3 GO:0045449,GO:0006350,GO:0004845,GO:0009116 2.4.2.9 P9WHK3 NC_000962.3 Mycobrowser_v5 CDS 1554188 1555480 . + 0 Rv1381 pyrC Involved in pyrimidine biosynthesis (third step) [catalytic activity: (S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate] Probable dihydroorotase PyrC (DHOase) Rv1381, (MTCY02B12.15), len: 430 aa. Probable pyrC, dihydroorotase, similar to many e.g. PYRC_BACCL|P46538 (40.5% identity in 395 aa overlap). Contains PS00483 Dihydroorotase signature 2. Belongs to the DHOase family. subfamily 2. P9WHL3 No intermediary metabolism and respiration P71809 P9WHL3 GO:0046872,GO:0006221,GO:0004151 3.5.2.3 P9WHL3 NC_000962.3 Mycobrowser_v5 CDS 1555477 1555974 . + 0 Rv1382 Rv1382 Unknown Probable export or membrane protein Rv1382, (MTCY02B12.16), len: 165 aa. Possible exported or membrane protein, hydrophobic domain at N-terminus. Predicted to be an outer membrane protein (See Song et al., 2008). P71810 No cell wall and cell processes P71810 NC_000962.3 Mycobrowser_v5 CDS 1744426 1744836 . - 0 Rv1542c glbN Oxygen transport Hemoglobin GlbN Rv1542c, (MTCY48.23), len: 136 aa. glbN, hemoglobin. Belongs to the protozoan/cyanobacterial globin family. Similar to myoglobins e.g. GLB_PARCA|P15160 myoglobin (hemoglobin) paramecium (116 aa), FASTA scores, opt: 284, E(): 2.1e -13, (35.7% identity in 115 aa overlap). Similar to Mycobacterium tuberculosis hypothetical globin, Rv2470. P9WN25 No intermediary metabolism and respiration 1IDR,1RTE,1S56,1S61,2GKM,2GKN,2GL3,2GLN P0A592 P9WN25 GO:0019825,GO:0015671,GO:0005344,GO:0020037 P9WN25 NC_000962.3 Mycobrowser_v5 CDS 2217659 2218036 . + 0 Rv1974 Rv1974 Unknown Probable conserved membrane protein Rv1974, (MTV051.12), len: 125 aa. Probable conserved membrane protein, weakly similar to other Mycobacterium tuberculosis proteins e.g. Rv1271c|Z77137|MTCY50.11 (113 aa), FASTA scores: opt: 98, E(): 1.4, (24.5% identity in 110 aa overlap); Rv1804c; Rv1690. Has possible signal peptide or transmembrane stretch from aa 12-30. Predicted to be an outer membrane protein (See Song et al., 2008). O53975 No cell wall and cell processes O53975 O53975 NC_000962.3 Mycobrowser_v5 CDS 1560445 1561269 . + 0 Rv1385 pyrF Involved in the biosynthesis of pyrimidines [catalytic activity: orotidine 5'-phosphate = UMP + CO(2)] Probable orotidine 5'-phosphate decarboxylase PyrF (OMP decarboxylase) (ompdecase) Rv1385, (MTCY21B4.02), len: 274 aa. Probable pyrF, orotidine 5'-phosphate decarboxylase, identical to DCOP_MYCBO|P42610 Mycobacterium bovis (274 aa). Contains PS00156 Orotidine 5'-phosphate decarboxylase active site. Belongs to the OMP decarboxylase family. P9WIU3 No intermediary metabolism and respiration P0A5M6 P9WIU3 GO:0004590,GO:0006221,GO:0006207 4.1.1.23 P9WIU3 NC_000962.3 Mycobrowser_v5 CDS 1561464 1561772 . + 0 Rv1386 PE15 Function unknown PE family protein PE15 Rv1386, (MTCY21B4.03), len: 102 aa. PE15, Member of Mycobacterium tuberculosis PE family (see Brennan & Delogu 2002), similar to many e.g. G913039 ORF 3' of PGRS tandem repeat (polymorphic GC-rich sequence) (100 aa), FASTA scores: opt: 149, E(): 0.0013, (31.5% identity in 92 aa overlap); also similar to Q49943|U1756A (99 aa) (34.7% identity in 95 aa overlap) and G466937|U1620K (100 aa) (36.2% identity in 69 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). P9WIH1 No PE/PPE P0A682 P9WIH1 P9WIH1 NC_000962.3 Mycobrowser_v5 CDS 1563694 1564266 . + 0 Rv1388 mihF Unknown Putative integration host factor MihF Rv1388, (MTCY21B4.05), len: 190 aa. Putative mihF, integration host factor. Almost identical to, but longer than, P96802|U75344 Mycobacterium smegmatis integration host factor (mIHF) for mycobacteriophage L5 (105 aa), FASTA scores: E(): 0, (96.1% identity in 102 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P71658 No information pathways P71658 P71658 NC_000962.3 Mycobrowser_v5 CDS 1564401 1565027 . + 0 Rv1389 gmk Essential for recycling GMP and indirectly, CGMP [catalytic activity: ATP + GMP = ADP + GDP] Probable guanylate kinase Gmk Rv1389, (MTCY21B4.06), len: 208 aa. Probable gmk, guanylate kinase, similar to e.g. KGUA_ECOLI|P24234 guanylate kinase from Escherichia coli (207 aa), FASTA scores: opt: 424, E(): 6.6e-20, (35.9% identity in 184 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00856 Guanylate kinase signature. Belongs to the guanylate kinase family. P9WKE9 No intermediary metabolism and respiration 1S4Q,1Z8F,1ZNW,1ZNX,1ZNY,1ZNZ P0A5I4 P9WKE9 GO:0005737,GO:0004385,GO:0006163,GO:0005524 2.7.4.8 P9WKE9 NC_000962.3 Mycobrowser_v5 CDS 1565093 1565425 . + 0 Rv1390 rpoZ Promotes RNA polymerase assembly. Latches the N-and C-terminal regions of the beta' subunit thereby faciltating its interaction with the beta and alpha subunits [catalytic activity: N nucleoside triphosphate = N diphosphate + {RNA}N]. Probable DNA-directed RNA polymerase (omega chain) RpoZ (transcriptase omega chain) (RNA polymerase omega subunit) Rv1390, (MTCY21B4.07), len: 110 aa. Probable rpoZ, DNA-directed RNA polymerase omega chain. Belongs to the RNA polymerase omega chain family. P9WGY5 No information pathways P66721 P9WGY5 GO:0003899,GO:0006351,GO:0003677 2.7.7.6 P9WGY5 NC_000962.3 Mycobrowser_v5 CDS 1583101 1584474 . + 0 Rv1407 fmu Unknown Probable Fmu protein (sun protein) Rv1407, (MTCY21B4.24), len: 457 aa. Probable fmu protein, similar to SUN_ECOLI|P36929 sun protein (fmu protein) from Escherichia coli (429 aa), FASTA scores: E(): 2.5e-20, (30.6% identity in 451 aa overlap). P9WGX3 No information pathways P71675 P9WGX3 GO:0008168,GO:0006355,GO:0003723 2.1.1.- P9WGX3 NC_000962.3 Mycobrowser_v5 CDS 1764979 1765383 . + 0 Rv1561 vapC11 Unknown Possible toxin VapC11 Rv1561, (MTCY48.04c), len: 134 aa. Possible vapC11, toxin, part of toxin-antitoxin (TA) operon with Rv1560, contains PIN domain, (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others from Mycobacterium tuberculosis e.g. Q10847|Rv2010|MTCY39.07c (132 aa), FASTA scores: (37.0% identity in 127 aa overlap); and O06566|Rv1114|MTCY22G8.03 (124 aa). P9WFA5 No virulence, detoxification, adaptation P64879 P9WFA5 P9WFA5 NC_000962.3 Mycobrowser_v5 CDS 1568109 1569587 . - 0 Rv1393c Rv1393c Function unknown; probably involved in cellular metabolism Probable monoxygenase Rv1393c, (MTCY21B4.10c), len: 492 aa. Probable monooxygenase, similar to others e.g. CYMO_ACISP|P12015 cyclohexanone monooxygenase from Acinetobacter sp. (542 aa), FASTA scores: E(): 0, (33.0% identity in 473 aa overlap); also to Rv3083|MTCY31.20|E241788 hypothetical 55.0 kDa protein from Mycobacterium tuberculosis (495 aa) (36.3% identity in 490 aa overlap); and Rv0565c, Rv3854c, Rv3049c, Rv0892. P71662 No intermediary metabolism and respiration P71662 P71662 GO:0055114,GO:0004497 1.14.13.- P71662 NC_000962.3 Mycobrowser_v5 CDS 1569584 1570969 . - 0 Rv1394c cyp132 Cytochromes P450 are a group of heme-thiolate monooxygenases. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Probable cytochrome P450 132 Cyp132 Rv1394c, (MT1439, MTCY21B4.11c), len: 461 aa. Probable cyp132, cytochrome P450 132. Some similarity to others e.g. CP4B_HUMAN|P13584 human cytochrome p450 (511 aa), FASTA scores: opt: 486, E(): 7.4e-21, (28.6% identity in 423 aa overlap); etc. Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. May belong to the cytochrome P450 family. Experimentally shown that the expression of cyp132 is induced by the transcriptional regulatory protein Rv1395 (Recchi et al., 2003). P9WPN3 No intermediary metabolism and respiration P77900 P9WPN3 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPN3 NC_000962.3 Mycobrowser_v5 CDS 1574850 1575809 . - 0 Rv1399c nlhH Function unknown, but supposedly involved in lipid metabolism Probable non lipolytic carboxylesterase NlhH Rv1399c, (MTCY21B4.16c), len: 319 aa. Possible nlhH, non lipolytic carboxylesterase, most similar to G695278 lipase like enzyme from Ralstonia eutropha (364 aa), FASTA scores: opt: 648, E(): 4.4e-34, (37.3% identity in 327 aa ov erlap), similar to Mycobacterium tuberculosis hypothetical lipases e.g. Rv2284, Rv2485c, Rv1426c, etc. Previously known as lipH. P9WK87 No intermediary metabolism and respiration P71667 P9WK87 GO:0016787 3.1.-.- P9WK87 NC_000962.3 Mycobrowser_v5 CDS 1589891 1590292 . + 0 Rv1414 Rv1414 Function unknown Conserved hypothetical protein Rv1414, (MTCY21B4.31), len: 133 aa. Conserved hypothetical protein, similar to C-terminal part of AB010956|AB010956_1 novel metal-activated pyridoxal enzyme from Arthrobacter sp. (379 aa), FASTA scores: opt: 163, E(): 0.00063, (32.1% identity in 112 aa overlap). Rv1413 is similar to N-terminal part of same enzyme suggesting possible frameshift. Sequence has been checked and no errors found, it is identical in Mycobacterium bovis strain AF2122/97 and in Mycobacterium tuberculosis CDC1551. P9WLY3 No conserved hypotheticals P9WLY3 P9WLY3 NC_000962.3 Mycobrowser_v5 CDS 1599658 1601037 . + 0 Rv1425 Rv1425 May be involved in synthesis of triacylglycerol Possible triacylglycerol synthase (diacylglycerol acyltransferase) Rv1425, (MTCY21B4.43,MTCY493.29c), len: 459 aa. Possible triacylglycerol synthase (See Daniel et al., 2004), similar to many M. tuberculosis proteins e.g. Rv3740c, Rv3734c, Rv1760, etc. P9WKC1 No lipid metabolism P71694 P9WKC1 GO:0006071,GO:0008610,GO:0004144 2.3.1.20 P9WKC1 NC_000962.3 Mycobrowser_v5 CDS 1572127 1573857 . - 0 Rv1396c PE_PGRS25 Function unknown PE-PGRS family protein PE_PGRS25 Rv1396c, (MTCY21B4.13c), len: 576 aa. PE_PGRS25, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002), strong similarity to many e.g. glycine rich protein MTCY130.10C|E245019 (603 aa), FASTA scores: opt: 1945, E(): 0, (57.5% identity in 619 aa overlap). Contains PS00017 ATP/GTP-binding site motif A, similar to other PGRS-type sequences. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P71664 No PE/PPE P71664 P71664 P71664 NC_000962.3 Mycobrowser_v5 CDS 1574510 1574767 . - 0 Rv1398c vapB10 Unknown Possible antitoxin VapB10 Rv1398c, (MTCY21B4.15c), len: 85 aa. Possible vapB10, antitoxin, part of toxin-antitoxin (TA) operon with Rv1397c (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. Rv2547|MTCY159.09C (85 aa), FASTA scores: E(): 0.0035, (37.1% identity in 62 aa overlap); Rv0581, Rv2871, Rv1241, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLZ1 No virulence, detoxification, adaptation P64835 P9WLZ1 GO:0006355,GO:0003677 P9WLZ1 NC_000962.3 Mycobrowser_v5 CDS 1575834 1576796 . - 0 Rv1400c lipI Function unknown, but possibly involved in lipid metabolism Probable lipase LipH Rv1400c, (MTCY21B4.17c), len: 320 aa. Possible lipI, lipase, most similar to G695278 lipase like enzyme (364 aa), FASTA sscores: opt: 611, E(): 3.5e-30, (36.6% identity in 352 aa overlap); similar to M. tuberculosis hypothetical lipases e.g. Rv1399c|MTCY21B4.16c (58.1% identical in 315 aa overlap); Rv1426c, Rv2284, etc. P71668 No intermediary metabolism and respiration P71668 P71668 GO:0008152,GO:0016787 3.1.-.- P71668 NC_000962.3 Mycobrowser_v5 CDS 1576930 1577532 . + 0 Rv1401 Rv1401 Unknown Possible membrane protein Rv1401, (MTCY21B4.18), len: 200 aa. Possible membrane protein. P9WG51 No cell wall and cell processes P64837 P9WG51 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1577613 1579580 . + 0 Rv1402 priA Recognizes a specific hairpin sequence on PHIX SSDNA; this structure is then recognized and bound by proteins PRIB and PRIC. Formation of the primosome proceeds with the subsequent actions of DNAB, DNAC, DNAT and primase. PRIA then functions as a helicase within the primosome Putative primosomal protein N' PriA (replication factor Y) Rv1402, (MTCY21B4.19), len: 655 aa. Putative priA, primosomal protein N'. Similar to e.g. PRIA_ECOLI|P17888 primosomal protein N' (replication factor Y) (732 aa), FASTA scores, opt: 386, E(): 1.3e-16, (27.6% identity in 711 aa overlap). Compared to other bacterial priA, it has a very divergent helicase domain. Belongs to the helicase family. PRIA subfamily. P9WMQ9 No information pathways P9WMQ9 GO:0003677,GO:0006269,GO:0005658,GO:0004386,GO:0046872,GO:0005524 3.6.4.- P9WMQ9 NC_000962.3 Mycobrowser_v5 CDS 1695943 1696443 . - 0 Rv1506c Rv1506c Unknown Hypothetical protein Rv1506c, (MTCY277.28c), len: 166 aa. Hypothetical unknown protein. P71785 No unknown P71785 P71785 P71785 NC_000962.3 Mycobrowser_v5 CDS 1580591 1581073 . + 0 Rv1404 Rv1404 Involved in transcriptional mechanism Probable transcriptional regulatory protein Rv1404, (MTCY21B4.21), len: 160 aa. Probable transcriptional regulatory protein, some similarity to MARR_ECOLI|P27245 multiple antibiotic resistance protein from Escherichia coli (125 aa), FASTA scores: opt: 136, E(): 0.004, (35.1% identity in 74 aa overlap). P71672 No regulatory proteins 2NYX P71672 P71672 GO:0006350,GO:0006355,GO:0003700,GO:0005622 P71672 NC_000962.3 Mycobrowser_v5 CDS 1581145 1581969 . - 0 Rv1405c Rv1405c Causes methylation Putative methyltransferase Rv1405c, (MTCY21B4.22c), len: 274 aa. Putative methyltransferase, most similar to PMTA_RHOSH|Q05197 phosphatidylethanolamine m-methyltransferase (203 aa), FASTA scores: opt: 219, E(): 2.6e-07, (29.9% identity in 144 aa overlap); similar to Rv1403c|MTCY21B4.20c (59.3% identity in 273 aa overlap), Rv1523, Rv2952, etc. P9WLY7 No intermediary metabolism and respiration P64841 P9WLY7 GO:0008168,GO:0008152 P9WLY7 NC_000962.3 Mycobrowser_v5 CDS 1584499 1585197 . + 0 Rv1408 rpe Involved in the calvin cycle [catalytic activity: D-ribulose 5-phosphate = D-xylulose 5- phosphate] Probable ribulose-phosphate 3-epimerase Rpe (PPE) (R5P3E) (pentose-5-phosphate 3-epimerase) Rv1408, (MTCY21B4.25), len: 232 aa. Probable rpe, ribulose-phosphate 3-epimerase, similar to many e.g. CXEC_ALCEU|P40117 (241 aa), FASTA scores: opt: 638, E(): 1.5e-34, (48.3% identity in 234 aa overlap); and RPE_ECOLI|P32661 ribulose-phosphate 3-epimerase (225 aa), FASTA scores: E(): 0, (46.2% identity in 221 aa overlap). Contains PS01085 Ribulose-phosphate 3-epimerase family signature 1. Belongs to the ribulose-phosphate 3-epimerase family. P9WI51 No intermediary metabolism and respiration P65760 P9WI51 GO:0004750,GO:0005975 5.1.3.1 P9WI51 NC_000962.3 Mycobrowser_v5 CDS 1601059 1602321 . - 0 Rv1426c lipO Function unknown, but supposedly involved in lipid metabolism Probable esterase LipO Rv1426c, (MTCY493.28), len: 420 aa. Possible lipO, esterase, similar to several Mycobacterium tuberculosis hypothetical lipases and esterases e.g. Rv1399c, Rv2284, etc. Also similar in central region to AAAD_HUMAN|P22760 human arylacetamide deacetylase (398 aa), FASTA scores: opt:210, E(): 7.6e-07, (29.3% identity in 191 aa overlap). O06832 No intermediary metabolism and respiration O06832 O06832 GO:0016787 3.1.-.- O06832 NC_000962.3 Mycobrowser_v5 CDS 1608083 1609852 . + 0 Rv1431 Rv1431 Unknown Conserved membrane protein Rv1431, (MTCY493.23c), len: 589 aa. Conserved membrane protein, shows strong similarity to another M. tuberculosis hypothetical protein Rv1132|MTCY22G8.21 (48.2% identity in 585 aa overlap). O06827 No cell wall and cell processes O06827 O06827 NC_000962.3 Mycobrowser_v5 CDS 1586210 1587766 . - 0 Rv1410c Rv1410c Involved in transport of aminoglycosides and tetracycline across the membrane (export): drug resistance by an export mechanism (conferes resistance to toxic compounds by removing them for the cells). Responsible for the translocation of the substrate across the membrane. Aminoglycosides/tetracycline-transport integral membrane protein Rv1410c, (MTCY21B4.27c), len: 518 aa. Aminoglycoside/tetracycline-transport integral membrane protein (see citation below), member of major facilitator superfamily (MFS), similar to others e.g. AC22_STRCO|P46105 probable actinorhodin transporter from Streptomyces coelicolor (578 aa), FASTA scores: opt: 442, E(): 4.9e-21, (28.5% identity in 466 aa overlap); etc. Contains PS00216 Sugar transport proteins signature 1. Could be termed P55. Note that the Rv1410c-Rv1411c operon seems transcribed from two promoters in Mycobacterium bovis BCG (see Bigi et al., 2000). P9WJY3 No cell wall and cell processes P71678 P9WJY3 GO:0005886,GO:0055085,GO:0005215,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1588567 1589172 . + 0 Rv1412 ribC Involved in riboflavin synthesis. Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyzes the dismutation of 6,7-dimethyl-8-lumazine to riboflavin and 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)- pyrimidinedione. Probable riboflavin synthase alpha chain RibC (RibE) Rv1412, (MTCY21B4.29), len: 201 aa. Probable ribC (ribE), Riboflavin synthase alpha chain, strong similarity to e.g. RISA_ACTPL|P50854 (215 aa), FASTA scores: opt: 586, E(): 1.8e-33, (50.8% identity in 197 aa overlap). Contains 2 x PS00693 Riboflavin synthase alpha chain family signature. P9WK35 No intermediary metabolism and respiration P65327 P9WK35 GO:0004746,GO:0009231 2.5.1.9 P9WK35 NC_000962.3 Mycobrowser_v5 CDS 1590397 1591674 . + 0 Rv1415 ribA2 Involved in riboflavin biosynthesis [catalytic activity: GTP + 3 H(2)O = formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate]. Probable riboflavin biosynthesis protein RibA2 : GTP cyclohydrolase II + 3,4-dihydroxy-2-butanone 4-phosphate synthase (DHBP synthase) Rv1415, (MTCY21B4.33), len: 425 aa. Probable ribA2, Riboflavin biosynthesis protein, similar to many e.g. GCH2_BACSU|P17620 Bacillus subtilis (398 aa), FASTA scores: opt: 1388, E(): 0, (55.4% identity in 399 aa overlap). Also similar to second Mycobacterium tuberculosis gtp cyclohydrolase Rv1940|ribA1 (353 aa). In the N-terminal section; belongs to the DHBP synthase family. In the C-terminal section; belongs to the GTP cyclohydrolase II family. P9WHF1 No intermediary metabolism and respiration P0A5V0 P9WHF1 GO:0005525,GO:0003935,GO:0000287,GO:0030145,GO:0009231,GO:0008270,GO:0008686 3.5.4.25,4.1.99.12 P9WHF1 NC_000962.3 Mycobrowser_v5 CDS 1623287 1623697 . - 0 Rv1444c Rv1444c Unknown Unknown protein Rv1444c, (MTCY493.10), len: 136 aa. Unknown protein. O06815 No conserved hypotheticals O06815 NC_000962.3 Mycobrowser_v5 CDS 1596881 1597909 . + 0 Rv1422 Rv1422 Function unknown Conserved hypothetical protein Rv1422, (MTCY21B4.40), len: 342 aa. Conserved hypothetical protein, similar to many hypothetical proteins e.g. YAMB_THETU|P38541 Thermoanaerobacterium thermosulfurigenes (323 aa), FASTA scores: opt: 519, E(): 1.6e-25, (33.1% identity in 320 aa overlap); and AF106003|AF106003_3 Streptomyces coelicolor (363 aa), FASTA scores: opt: 1047, E(): 0, (54.5% identity in 308 aa overlap). P9WMU5 No conserved hypotheticals P71691 P9WMU5 P9WMU5 NC_000962.3 Mycobrowser_v5 CDS 1598893 1599654 . - 0 Rv1424c Rv1424c Unknown Possible membrane protein Rv1424c, (MTCY21B4.42c,MTCY493.30), len: 253 aa. Possible membrane protein, contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLX7 No cell wall and cell processes P9WLX7 NC_000962.3 Mycobrowser_v5 CDS 1602321 1603928 . - 0 Rv1427c fadD12 Function unknown, but supposed involvement in lipid degradation. Possible long-chain-fatty-acid--CoA ligase FadD12 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv1427c, (MTCY493.27), len: 535 aa. Possible fadD12, long-chain-fatty-acid-CoA synthetase, similar to many e.g. NP_302632.1|NC_002677 acyl-CoA synthase from Mycobacterium leprae (548 aa); AAD01929.2|AF031419 putative long-chain-fatty-acid--CoA ligase from Pseudomonas putida (565 aa); NP_419782.1|NC_002696 putative long-chain-fatty-acid--CoA ligase from Caulobacter crescentus (530 aa); PC60_YEAST|P38137 yeast peroxisomal-coenzyme A synthetase (543 aa), FASTA scores: opt: 507, E(): 2.9e-25, (30.4% identity in 365 aa overlap). Also similar to many M. tuberculosis proteins e.g. MTCY06A4.14 (44.8% identity in 525 aa overlap). Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family. O06831 No lipid metabolism O06831 O06831 GO:0008152,GO:0016874 6.2.1.- O06831 NC_000962.3 Mycobrowser_v5 CDS 1603932 1604759 . - 0 Rv1428c Rv1428c Function unknown Conserved hypothetical protein Rv1428c, (MTCY493.26), len: 275 aa. Conserved hypothetical protein, some similarity to hypothetical proteins from Mycobacterium tuberculosis e.g. Rv0502|YV29_MYCTU|Q11167 (358 aa), FASTA scores: opt: 355, E(): 5e-16, (32.6% identity in 273 aa overlap); and Rv1920. O06830 No conserved hypotheticals O06830 O06830 GO:0008152,GO:0008415 O06830 NC_000962.3 Mycobrowser_v5 CDS 2387202 2387972 . - 0 Rv2126c PE_PGRS37 Function unknown PE-PGRS family protein PE_PGRS37 Rv2126c, (MTCY261.22c), len: 256 aa. PE_PGRS37, Possible PE_PGRS pseudogene fragment, similar to the Gly-rich C-terminus of many members of the Mycobacterium tuberculosis PGRS family. L0TBL4 No PE/PPE L0TBL4 NC_000962.3 Mycobrowser_v5 CDS 3177822 3178085 . + 0 Rv2866 relG Function unknown Toxin RelG Rv2866, (MTV003.12), len: 87 aa. RelG, toxin, part of toxin-antitoxin (TA) operon with Rv2865 (See Pandey and Gerdes, 2005), similar to O50461|Rv1246c|MTV006.18c conserved hypothetical protein from Mycobacterium tuberculosis (97 aa), FASTA scores: opt: 290, E(): 3.6e-16, (54.1% identity in 85 aa overlap). O33348 No virulence, detoxification, adaptation 3G5O O33348 O33348 O33348 NC_000962.3 Mycobrowser_v5 CDS 1604878 1606146 . + 0 Rv1429 Rv1429 Function unknown Conserved protein Rv1429, (MTCY493.25c), len: 422 aa. Conserved protein, some similarity to transcriptional regulator proteins e.g. CDAR_ECOLI|P37047 Carbohydrate diacid regulator from Escherichia coli (391 aa), FASTA scores: opt: 210, E(): 3e-06, (27.7% identity in 296 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins Rv2370c, Rv1194c, Rv1453, Rv2242, and Rv1186c. O06829 No conserved hypotheticals O06829 O06829 NC_000962.3 Mycobrowser_v5 CDS 1609849 1611270 . + 0 Rv1432 Rv1432 Function unknown; probably involved in cellular metabolism Probable dehydrogenase Rv1432, (MTCY493.22c), len: 473 aa. Probable dehydrogenase, shows strong similarity to P49_STRLI|P06108 p49 protein from Streptomyces lividans (469 aa), FASTA scores: opt: 1362, E(): 0, (44.9% identity in 474 aa overlap); and weak similarity to other dehydrogenases. O06826 No intermediary metabolism and respiration O06826 O06826 GO:0016491,GO:0055114 1.-.-.- O06826 NC_000962.3 Mycobrowser_v5 CDS 1611434 1612249 . + 0 Rv1433 Rv1433 Unknown Possible conserved exported protein Rv1433, (MTCY493.21c), len: 271 aa. Possible exported protein with N-terminal signal sequence, highly similar to Q49706 hypothetical protein from Mycobacterium leprae (271 aa), FASTA scores: opt: 1341, E(): 0, (68.3% identity in 271 aa overlap). Also shows similarity to M. tuberculosis lipoprotein Rv2518c|MTV009.03c lppS (408 aa) (40.0% identity in 230 aa overlap); and others e.g. Rv0116c, Rv0192, Rv2518c, Rv0483. Predicted to be an outer membrane protein (See Song et al., 2008). O06825 No cell wall and cell processes O06825 O06825 O06825 NC_000962.3 Mycobrowser_v5 CDS 1623714 1624457 . - 0 Rv1445c devB Involved in pentose phosphate pathway. Hydrolysis of 6-phosphogluconolactone to 6- phosphogluconate. [catalytic activity: 6-phospho-D-glucono-1,5-lactone + H(2)O = 6- phospho-D-gluconate] Probable 6-phosphogluconolactonase DevB (6PGL) Rv1445c, (MTCY493.09), len: 247 aa. Possible devB (PGL), 6-phosphogluconolactonase, belongs to a different family to the upstream gene zwf2. Similar to e.g. DEVB_ANASP|P46016 putative glucose-6-phosphate 1-dehydrogenase (239 aa), FASTA scores: opt: 439, E(): 2.6e-20, (34.0% identity in 247 aa overlap). Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily. P9WQP5 No intermediary metabolism and respiration 3ICO P63338 P9WQP5 GO:0006098,GO:0017057 3.1.1.31 P9WQP5 NC_000962.3 Mycobrowser_v5 CDS 1652768 1653256 . + 0 Rv1465 Rv1465 Function unknown; probably involved in cellular metabolism. Possible nitrogen fixation related protein Rv1465, (MTV007.12), len: 162 aa. Possible nitrogen fixation related protein. Equivalent to Z99125|MLCL536.24c nitrogen fixation protein NIFU from Mycobacterium leprae (165 aa), FASTA scores: opt: 870, E(): 0, (81.8% identity in 165 aa overlap). Also similar to O32163|Z99120|NIFU_BACSU NifU-like protein from Bacillus subtilis (147 aa), FASTA scores: opt: 354, E(): 4.1e-17, (38.3% identity in 141 aa overlap) and to AL096839|SCC22.02 hypothetical protein from Streptomyces coelicolor (156 aa), FASTA scores: opt: 569, E(): 1.2e-31, (56.3% identity in 158 aa overlap). O53156 No intermediary metabolism and respiration O53156 O53156 GO:0016226,GO:0051536,GO:0005515,GO:0005506 O53156 NC_000962.3 Mycobrowser_v5 CDS 1846716 1846973 . - 0 Rv1638A Rv1638A Function unknown Conserved hypothetical protein Rv1638A, len: 85 aa. Conserved hypothetical protein, similar to C-terminal part of P31511|35KD_MYCTU 35kd immunogenic protein from Mycobacterium tuberculosis (270 aa), FASTA scores: opt: 159, E(): 0.002, (50.90% identity in 55 aa overlap); and to Mycobacterium leprae ML0981 possible pseudogene, an orthologue of 35kd immunogenic protein from Mycobacterium tuberculosis. Size difference suggests possible gene fragment. L7N673 No conserved hypotheticals L7N673 NC_000962.3 Mycobrowser_v5 CDS 1614329 1615567 . + 0 Rv1437 pgk Involved in the second phase of glycolysis (second step) [catalytic activity: ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate] Probable phosphoglycerate kinase Pgk Rv1437, (MTCY493.17c), len: 412 aa. Probable pgk, Phosphoglycerate kinase, highly similar to many e.g. PGK_MYCLE|P46712 Mycobacterium leprae (416 aa), FASTA scores: opt: 2153, E(): 0, (80.4% identity in 414 aa overlap). Contains PS00111 Phosphoglycerate kinase signature. Belongs to the phosphoglycerate kinase family. P9WID1 No intermediary metabolism and respiration P65700 P9WID1 GO:0005737,GO:0006096,GO:0004618,GO:0005524 2.7.2.3 P9WID1 NC_000962.3 Mycobrowser_v5 CDS 1615564 1616349 . + 0 Rv1438 tpi Plays an important role in several metabolic pathways [catalytic activity: D-glyceraldehyde 3-phosphate = glycerone phosphate] Probable triosephosphate isomerase Tpi (TIM) Rv1438, (MTCY493.16c), len: 261 aa. Probable tpi (tpiA), Triosephosphate isomerase, highly similar to many e.g. TPIS_MYCLE|P46711 Mycobacterium leprae (261 aa), FASTA scores: opt: 1456, E(): 0, (83.9% identity in 261 aa overlap). Contains PS00171 Triosephosphate isomerase active site. Belongs to the triosephosphate isomerase family. P9WG43 No intermediary metabolism and respiration 3GVG,3TA6,3TAO P66940 P9WG43 GO:0006094,GO:0006096,GO:0006098,GO:0004807,GO:0005737 5.3.1.1 P9WG43 NC_000962.3 Mycobrowser_v5 CDS 1617837 1618070 . + 0 Rv1440 secG Involved in protein export. Participates in a early event of protein translocation. Probable protein-export membrane protein (translocase subunit) SecG Rv1440, (MTCY493.14c), len: 77 aa. Probable secG, protein-export membrane protein (translocase subunit) (see citation below), similar to many e.g. P38388|SECG_MYCLE probable protein-export membrane (77 aa), FASTA scores: opt: 450, E(): 6.7e-24, (96.1% identity in 77 aa overlap). Start changed since original submission (-40 aa). Part of the prokaryotic protein translocation apparatus which comprise SECA|Rv3240c, SECD|Rv2587c, SECE|Rv0638, SECF|Rv2586c, SECG and SECY|Rv0732. P9WGN5 No cell wall and cell processes P66791 P9WGN5 GO:0016021,GO:0005886,GO:0009306,GO:0055085,GO:0015450 NC_000962.3 Mycobrowser_v5 CDS 1618209 1619684 . - 0 Rv1441c PE_PGRS26 Function unknown PE-PGRS family protein PE_PGRS26 Rv1441c, (MTCY493.13), len: 491 aa. PE_PGRS26, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002), similar to Y0DP_MYCTU|Q50615 hypothetical glycine-rich 40.8 kDa protein (498 aa), fasta scores: opt: 1625, E(): 0, (55.2% identity in 518 aa overlap). Q79FP3 No PE/PPE Q79FP3 Q79FP3 Q79FP3 NC_000962.3 Mycobrowser_v5 CDS 1619791 1622091 . + 0 Rv1442 bisC This enzyme may serve as a scavenger, allowing the cell to utilize biotin sulfoxide as a biotin source Probable biotin sulfoxide reductase BisC (BDS reductase) (BSO reductase) Rv1442, (MTCY493.12c), len: 766 aa. Probable bisC, Biotin sulfoxide reductase, similar to BISC_ECOLI|P20099 biotin sulfoxide reductase from Escherichia coli (739 aa), FASTA scores: opt: 1271, E():0, (40.2% identity in 744 aa overlap). O06817 No intermediary metabolism and respiration O06817 O06817 GO:0055114,GO:0016491,GO:0030151 1.-.-.- O06817 NC_000962.3 Mycobrowser_v5 CDS 1622207 1622692 . - 0 Rv1443c Rv1443c Unknown Unknown protein Rv1443c, (MTCY493.11), len: 161 aa. Unknown protein. O06816 No conserved hypotheticals O06816 O06816 O06816 NC_000962.3 Mycobrowser_v5 CDS 1678942 1679172 . + 0 Rv1489A Rv1489A Function unknown Conserved protein Rv1489A, len: 76 aa. Conserved protein, similar to part of alpha subunit of many methylmalonyl-CoA mutases (~750 aa). Size difference suggests possible gene fragment although Mycobacterium tuberculosis has intact methylmalonyl-CoA mutase gene. P71774|MUTB_MYCTU probable methylmalonyl-CoA mutase from Mycobacterium tuberculosis (750 aa), FASTA scores: opt: 258, E(): 3.2e-10, (73.35% identity in 60 aa overlap). ORF predicted by GC plot. L7N6A8 No conserved hypotheticals Q8VK12 L7N6A8 GO:0016866,GO:0008152,GO:0031419 L7N6A8 NC_000962.3 Mycobrowser_v5 CDS 1624454 1625365 . - 0 Rv1446c opcA May be involved in the functional assembly of glucose 6-phosphate dehydrogenase Putative OXPP cycle protein OpcA Rv1446c, (MTCY493.08), len: 303 aa. Putative opcA, OxPP cycle protein. Highly similar to S72774 B1496_F1_30 protein from Mycobacterium leprae (265 aa), FASTA scores: opt: 1056, E(): 0, (70.3% identity in 239 aa overlap). Also similar to OPCA_NOSS2|P48971 putative oxppcycle protein opca from Nostoc punctiforme (465 aa), fasta scores: opt: 177, E(): 7.3e-05, (23.4% identity in 321 aa overlap). Aids in G6PD activity. O06813 No intermediary metabolism and respiration O06813 O06813 NC_000962.3 Mycobrowser_v5 CDS 1626959 1628080 . - 0 Rv1448c tal Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway [catalytic activity: sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-erythrose 4-phosphate + D-fructose 6-phosphate] Probable transaldolase Tal Rv1448c, (MTCY493.06), len: 373 aa. Probable tal, Transaldolase, highly similar to many e.g. TAL_MYCLE|P55193 transaldolase from Mycobacterium leprae (375 aa), FASTA scores: opt: 1891, E(): 0, (78.6% identity in 370 aa overlap). Belongs to the transaldolase family. P9WG33 No intermediary metabolism and respiration O06812 P9WG33 GO:0006098,GO:0004801,GO:0005737 2.2.1.2 P9WG33 NC_000962.3 Mycobrowser_v5 CDS 1630638 1634627 . - 0 Rv1450c PE_PGRS27 Function unknown PE-PGRS family protein PE_PGRS27 Rv1450c, (MTCY493.04), len: 1329 aa. PE_PGRS27, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), similar to Y03A_MYCTU|Q10637 hypothetical glycine-rich 49.6 kDa protein (603 aa), fasta scores: opt: 2112, E(): 0, (56.5% identity in 630 aa overlap). Q79FP2 No PE/PPE Q79FP2 Q79FP2 Q79FP2 NC_000962.3 Mycobrowser_v5 CDS 1635029 1635955 . + 0 Rv1451 ctaB Thought to be involved in aerobic respiration. Probable cytochrome C oxidase assembly factor CtaB Rv1451, (MTCY493.03c), len: 308 aa. Probable ctaB, cytochrome C oxidase assembly factor, and integral membrane protein. Highly similar to several Mycobacterium leprae proteins e.g. Q49685 CYOE cytochrome O ubiquinol oxidase assembly factor (300 aa), FASTA scores: opt: 1636, E(): 0, (82.7% identity in 307 aa overlap); NP_301495.1|NC_002677 putative protoheme IX farnesyltransferase (321 aa); NP_301495.1|NC_002677 putative protoheme IX farnesyltransferase (321 aa). P9WFR7 No intermediary metabolism and respiration O06809 P9WFR7 GO:0016021,GO:0005886,GO:0008495,GO:0048034 2.5.1.- NC_000962.3 Mycobrowser_v5 CDS 1658980 1659354 . + 0 Rv1470 trxA Thioredoxin participates in various redox reactions through the reversible oxidation of its active center dithiol, to a disulfide, & catalyzes dithiol-disulfide exchange reactions Probable thioredoxin TrxA Rv1470, (MTV007.17), len: 124 aa. Probable trxA, thioredoxin, similar to many e.g. P12243|THI1_SYNP7 thioredoxin 1 from Synechococcus sp. (106 aa), FASTA scores: opt: 201, E(): 9.2e-08, (35.4% identity in 99 aa overlap); etc. Highly similar to downstream ORF Rv1471|trxB1 probable thioredoxin from Mycobacterium tuberculosis (123 aa), FASTA scores: opt: 402, E(): 0, (54.4% identity in 114 aa overlap). Warning: note that Rv3914|MT4033|MTV028.05|trxC can be alternatively named trxA. O53161 No intermediary metabolism and respiration O53161 O53161 GO:0055114,GO:0016491,GO:0045454 1.-.-.- O53161 NC_000962.3 Mycobrowser_v5 CDS 1638381 1639646 . + 0 Rv1453 Rv1453 Involved in transcriptional mechanism Possible transcriptional activator protein Rv1453, (MTCY493.01c), len: 421 aa. Possible transcriptional activator, similar to Q50018 putative transcriptional activator trx from Mycobacterium leprae (517 aa), FASTA scores: opt: 1719, E(): 0, (54.0% identity in 500 aa overlap). Also highly similar to Mycobacterium tuberculosis proteins Rv2370c, Rv1194c, Rv2242, Rv1186c, and to the further upstream ORF's Rv1429|MTCY493.25c (28.1% identity in 335 aa overlap). Start changed since first submission (-11 aa). O06807 No regulatory proteins O06807 O06807 NC_000962.3 Mycobrowser_v5 CDS 1760997 1761605 . + 0 Rv1556 Rv1556 Possibly involved in a transcriptional mechanism Possible regulatory protein Rv1556, (MTCY48.09c), len: 202 aa. Possible regulatory protein, similar to X86780|SHGCPIR2|g987088 orfY, regulator of antibiotic transport complexes from Streptomyces hygroscopicus (204 aa), FASTA score: opt: 251, E(): 1.7e-10, (33.8% identity in 201 aa overlap) and others. P9WMD1 No regulatory proteins P67436 P9WMD1 GO:0006350,GO:0003700,GO:0006355 P9WMD1 NC_000962.3 Mycobrowser_v5 CDS 1639674 1640660 . - 0 Rv1454c qor Catalyzes the one electron reduction of certain quinones [catalytic activity: NADPH + quinone = NADP+ + semiquinone] Probable quinone reductase Qor (NADPH:quinone reductase) (zeta-crystallin homolog protein) Rv1454c, (MTV007.01c), len: 328 aa. Probable qor, quinone oxidoreductase, simiar to U87282|RCU87282_2 quinone oxidoreductase from Rhodobacter capsulatus (323 aa), FASTA scores: opt: 849, E(): 0, (44.7% identity in 329 aa overlap). Also similar to MTCY180.06 Hypothetical protein from Mycobacterium tuberculosis (334 aa), FASTA scores: opt: 430, E(): 2e-14, (32.3% identity in 350 aa overlap). Contains PS01162 Quinone oxidoreductase / zeta-crystallin signature. O53146 No intermediary metabolism and respiration O53146 O53146 GO:0055114,GO:0008270,GO:0003960 1.6.5.5 O53146 NC_000962.3 Mycobrowser_v5 CDS 1641493 1642425 . - 0 Rv1456c Rv1456c Thought to be involved in active transport of antibiotic across the membrane (export): unidentified antibiotic resistance by an export mechanism. Responsible for the translocation of the substrate across the membrane. Probable unidentified antibiotic-transport integral membrane ABC transporter Rv1456c, (MTV007.03c), len: 310 aa. Possible unidentified antibiotic-transport integral membrane protein ABC transporter (see citation below), equivalent to Z99125|MLCL536.34 from Mycobacterium leprae (311 aa), FASTA scores: opt: 1607, E(): 0, (83.3% identity in 300 aa overlap). O53148 No cell wall and cell processes O53148 O53148 GO:0006461,GO:0016021 O53148 NC_000962.3 Mycobrowser_v5 CDS 1642537 1643322 . - 0 Rv1457c Rv1457c Thought to be involved in active transport of antibiotic across the membrane (export): unidentified antibiotic resistance by an export mechanism. Responsible for the translocation of the substrate across the membrane. Probable unidentified antibiotic-transport integral membrane ABC transporter Rv1457c, (MTV007.04c), len: 261 aa. Possible unidentified antibiotic-transport integral membrane protein ABC transporter (see citation below), equivalent to Z99125|MLCL536.32 from Mycobacterium leprae (265 aa), FASTA scores: opt: 1415, E(): 0, (83.1% identity in 260 aa overlap). O86349 No cell wall and cell processes O86349 O86349 GO:0016020 NC_000962.3 Mycobrowser_v5 CDS 1643319 1644260 . - 0 Rv1458c Rv1458c Thought to be involved in active transport of antibiotic across the membrane (export): unidentified antibiotic resistance by an export mechanism. Responsible for energy coupling to the transport system. Probable unidentified antibiotic-transport ATP-binding protein ABC transporter Rv1458c, (MTV007.05c), len: 313 aa. Possible unidentified antibiotic-transport ATP-binding protein ABC transporter (see citation below), equivalent to Z99125|MLCL536.31 from Mycobacterium leprae (315 aa), FASTA scores: opt: 1812, E(): 0, (88.0% identity in 308 aa overlap). Similar to AF027770|AF027770_7 ABC-type transporter in FxbA region in Mycobacterium smegmatis (284 aa), FASTA scores: opt: 1412, E(): 0, (85.1% identity in 248 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). O53149 No cell wall and cell processes O53149 O53149 GO:0016887,GO:0005524 O53149 NC_000962.3 Mycobrowser_v5 CDS 2183372 2183869 . + 0 Rv1932 tpx Has antioxidant activity. Could remove peroxides or H(2)O(2) Probable thiol peroxidase Tpx Rv1932, (MTCY09F9.32c), len: 165 aa. Probable tpx (alternate gene name: cfp20), thiol peroxidase similar to TPX_ECOLI|P37901 thiol peroxidase (p20) from Escherichia coli (167 aa), fasta scores: opt: 535, E(): 7.3e-25, (52.4% identity in 164 aa overlap). There are four other related enzymes in M. tuberculosis: Rv2428, Rv2521, Rv2238c, Rv1608c. P9WG35 No virulence, detoxification, adaptation 1XVQ,1Y25 P66952 P9WG35 GO:0055114,GO:0008379,GO:0045454 1.11.1.- P9WG35 NC_000962.3 Mycobrowser_v5 CDS 1653231 1653578 . + 0 Rv1466 Rv1466 Function unknown Conserved protein Rv1466, (MTV007.13), len: 115 aa. Conserved protein. Equivalent to Z99125|MLCL536.23c hypothetical protein from Mycobacterium leprae (115 aa), FASTA scores: opt: 648, E(): 0, (81.7% identity in 115 aa overlap). Similar to ORF's downstream of sigma factors in Streptococcus mutans and Streptococcus pneumoniae e.g. O06451 ORF3 downstream of RpoD (SPDNAGCPO) (109 aa). Alternative TTG start possible at 13757 then avoids overlap with MTV007.12. O53157 No conserved hypotheticals O53157 O53157 O53157 NC_000962.3 Mycobrowser_v5 CDS 1646186 1646992 . + 0 Rv1460 Rv1460 Involved in transcriptional mechanism Probable transcriptional regulatory protein Rv1460, (MTV007.07), len: 268 aa. Probable transcriptional regulatory protein. Equivalent to Z99125|MLCL536.29c hypothetical protein from Mycobacterium leprae (254 aa), FASTA scores: opt: 1273, E(): 0, (79.6% identity in 250 aa overlap). Possible helix-turn-helix motif between aa 68 - 89. Start changed since original submission. O53151 No regulatory proteins O53151 O53151 GO:0006355,GO:0003700,GO:0005622 O53151 NC_000962.3 Mycobrowser_v5 CDS 1649526 1650719 . + 0 Rv1462 Rv1462 Function unknown Conserved hypothetical protein Rv1462, (MTV007.09), len: 397 aa. Conserved hypothetical protein. Equivalent to MLCL536.27c|Z99125 hypothetical protein from Mycobacterium leprae (392 aa), FASTA scores: opt: 2059, E(): 0, (80.4% identity in 392 aa overlap). Also similar to nearby Mycobacterium tuberculosis hypothetical protein Rv1461. P9WFP5 No conserved hypotheticals O53153 P9WFP5 GO:0005515,GO:0016226 P9WFP5 NC_000962.3 Mycobrowser_v5 CDS 1650716 1651516 . + 0 Rv1463 Rv1463 Thought to be involved in active transport across the membrane. Responsible for energy coupling to the transport system. Probable conserved ATP-binding protein ABC transporter Rv1463, (MTV007.10), len: 266 aa. Probable conserved ATP-binding protein ABC transporter, equivalent to Z99125|MLCL536.26c putative ABC transporter ATP-binding protein from Mycobacterium leprae (260 aa), FASTA scores: opt: 1444, E(): 0, (86.0% identity in 267 aa overlap). Very similar to U38804|PPU38804_55 ATP-dependent transporter YCF16 from porphyra purpurea chloroplast (251 aa), FASTA scores: opt: 822, E(): 0, (52.4% identity in 248 aa overlap); and similar to others. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters). O53154 No cell wall and cell processes O53154 O53154 GO:0016887,GO:0006810,GO:0005524 O53154 NC_000962.3 Mycobrowser_v5 CDS 1655609 1656721 . - 0 Rv1468c PE_PGRS29 Function unknown PE-PGRS family protein PE_PGRS29 Rv1468c, (MTV007.15c), len: 370 aa. PE_PGRS29, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below). Q79FP0 No PE/PPE Q79FP0 Q79FP0 Q79FP0 NC_000962.3 Mycobrowser_v5 CDS 1669283 1670416 . + 0 Rv1479 moxR1 Involved in transcriptional mechanism Probable transcriptional regulatory protein MoxR1 Rv1479, (MTV007.26), len: 377 aa. Probable moxR1, transcriptional regulatory protein, similar to X96434|BBGIDBMOX_2 moxR regulator from Borrelia burgdorferi (329 aa), FASTA scores: opt: 850, E():0, (43.5% identity in 317 aa overlap); and P. denitrificans. Highly similar to MoxR homologs of Mycobacterium tuberculosis and Mycobacterium avium (but these both differ at C-terminus) e.g. Rv3692, Rv3164c, and AF0021|AF002133_6 Mycobacterium avium strain GIR10 (309 aa), FASTA scores: opt: 1181, E(): 0, (83.7% identity in 227 aa overlap). Also similar to O33173|AF006054 MoxR fragment from Mycobacterium tuberculosis (211 aa), FASTA scores: opt: 1305, E(): 0, (94.3% identity in 212 aa overlap). Note that previously known as moxR. Q79FN7 No regulatory proteins Q79FN7 Q79FN7 GO:0016887,GO:0005524 Q79FN7 NC_000962.3 Mycobrowser_v5 CDS 1671377 1672384 . + 0 Rv1481 Rv1481 Unknown Probable membrane protein Rv1481, (MTCY277.02), len: 335 aa. Probable membrane protein, highly similar to YS02_MYCAV|O07395 hypothetical 36.1 kDa protein mav335 from Mycobacterium avium (335 aa), FASTA scores: opt: 1904, E(): 0, (89.0% identity in 337 aa overlap). Similar to AF116251|AF116251_1 BatA protein from Bacteroides fragilis (327 aa), FASTA scores: opt: 317, E(): 2e-12, (26.5% identity in 340 aa overlap). P9WFJ7 No cell wall and cell processes P64855 P9WFJ7 GO:0005886,GO:0016021 P9WFJ7 NC_000962.3 Mycobrowser_v5 CDS 1697356 1697859 . + 0 Rv1507A Rv1507A Unknown Hypothetical protein Rv1507A, len: 167 aa. Hypothetical unknow protein. Shows weak similarity with C-terminus of Q9XHQ7|CDA9 cytidine deaminase 9 from Arabidopsis thaliana (Mouse-ear cress) (298 aa), FASTA scores: opt: 104, E(): 4.2, (33.6% identity in 133 aa overlap), blastp scores: Score: 77, Identities: 39/133 (29%), Positives: 62/133 (46%). L7N6B6 No unknown L7N6B6 NC_000962.3 Mycobrowser_v5 CDS 1762947 1763393 . + 0 Rv1558 Rv1558 Function unknown Conserved protein Rv1558, (MTCY48.07c), len: 148 aa. Conserved protein, similar to other Mycobacterial tuberculosis proteins e.g. P71854|MTCY03C7.09c|Rv3547 (151 aa), FASTA scores opt: 330, E(): 9.1e-17, (39.7% identity in 151 aa overlap); also Q11057|Rv1261c (149 aa), and O53328|Rv3178 (119 aa). Similar also to AF072709|AF072709_5 Hypothetical protein with a new amplifiable element AUD4 from Streptomyces lividans (149 aa), FASTA scores: opt: 695, E(): 0, (69.1% identity in 149 aa overlap). P9WP11 No conserved hypotheticals P64875 P9WP11 P9WP11 NC_000962.3 Mycobrowser_v5 CDS 1660656 1662284 . + 0 Rv1473 Rv1473 Thought to be involved in active transport of macrolide across the membrane (export). Macrolide antibiotics resistance by an export mechanism. Responsible for energy coupling to the transport system. Probable macrolide-transport ATP-binding protein ABC transporter Rv1473, (MTV007.20), len: 542 aa. Possible macrolide-transport ATP-binding protein ABC transporter (see citation below), possibly in EF-3 subfamily. Similar to many ABC-transporters e.g. D90909_48|YHES_HAEIN from Synechocystis sp. strain PCC6803 (574 aa), FASTA scores: opt: 870, E(): 0, (33.3% identity in 525 aa overlap); P44808|YHES_HAEIN from Haemophilus influenzae (638 aa), FASTA scores: opt: 706, E(): 0, (33.7% identity in 517 aa overlap); etc. Contains two PS00017 ATP/GTP-binding site motif A (P-loop), and two PS00211 ABC transporter family signatures. Belongs to the ATP-binding transport protein family (ABC transporters). O53164 No cell wall and cell processes O53164 O53164 GO:0016887,GO:0005524 O53164 NC_000962.3 Mycobrowser_v5 CDS 1778539 1779048 . + 0 Rv1571 Rv1571 Function unknown Conserved protein Rv1571, (MTCY336.32c), len: 169 aa. Conserved protein, similar at N-terminal region to Q49625|LEPB1170_C3_227 hypothetical protein from Mycobacterium leprae (104 aa), FASTA results: opt: 473, E(): 3.9e-24, (74.5% identity in 102 aa overlap). Identical to O06619|AF041819|AF041819_6 Mycobacterium bovis BCG (169 aa). O06619 No conserved hypotheticals O06619 GO:0003824,GO:0005622,GO:0016070 O06619 NC_000962.3 Mycobrowser_v5 CDS 1663215 1666046 . - 0 Rv1475c acn Involved in tricarboxylic acid cycle [catalytic activity: citrate = cis-aconitate + H2O] Probable iron-regulated aconitate hydratase Acn (citrate hydro-lyase) (aconitase) Rv1475c, (MTV007.22c), len: 943 aa. Probable acn, iron-regulated aconitate hydratase, similar to many e.g. P70920|ACON_BRAJA aconitate hydratase from Bradyrhizobium japonicum (906 aa), FASTA scores: opt:1912, E(): 0, (54.8% identity in 958 aa overlap); closest to AF0021|AF002133_2 Mycobacterium avium strain GIR10 (961 aa), FASTA scores: opt: 5072, E(): 0, (82.8% identity in 943 aa overlap). Note aconitase has an active (4FE-4S) and an inactive (3FE-4S) forms. The active (4FE-4S) cluster is part of the catalytic site that interconverts citrate, cis-aconitase, and isocitrate. O53166 No intermediary metabolism and respiration O53166 O53166 GO:0003994,GO:0008152,GO:0051539 4.2.1.3 O53166 NC_000962.3 Mycobrowser_v5 CDS 1666204 1666764 . + 0 Rv1476 Rv1476 Unknown Possible membrane protein Rv1476, (MTV007.23), len: 186 aa. Possibly membrane protein, TMhelix 138-60. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53167 No cell wall and cell processes O53167 O53167 NC_000962.3 Mycobrowser_v5 CDS 1747195 1747626 . + 0 Rv1546 Rv1546 Function unknown Conserved protein Rv1546, (MTCY48.19c), len: 143 aa. Conserved protein, similar to O05902|Rv0910|MTCY21C12.04 Hypothetical protein from Mycobacterium tuberculosis (144 aa), FASTA scores: E(): 5e-30, (37.3% identity in 142 aa overlap). P9WLU7 No conserved hypotheticals P64873 P9WLU7 P9WLU7 NC_000962.3 Mycobrowser_v5 CDS 2140486 2140704 . + 0 Rv1893 Rv1893 Function unknown Conserved hypothetical protein Rv1893, (MTCY180.25c), len: 72 aa. Conserved hypothetical protein. Equivalent to MLCB561.11|AL049571 hypothetical protein from Mycobacterium leprae (74 aa), FASTA scores: opt: 317, E(): 4.6e-15, (69.4% identity in 72 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O07739 No conserved hypotheticals O07739 NC_000962.3 Mycobrowser_v5 CDS 1668419 1669144 . + 0 Rv1478 Rv1478 Unknown, but supposedly involved in virulence Possible invasion protein Rv1478, (MTV007.25), len: 241 aa. Possible invasion protein. Possibly exported protein, nearly identical to AF0060|AF006054_2 hypothetical invasion protein INV2 of Mycobacterium tuberculosis (240 aa), FASTA scores: opt: 1509, E(): 0, (95.0% identity in 241 aa overlap); very similar to AF0021|AF002133_5 hypothetical invasion protein INV2 from Mycobacterium avium (244 aa), FASTA scores: opt: 1269, E():0, (78.0% identity in 246 aa overlap). Also similar to Mycobacterium tuberculosis protein MTCY336.37 and weakly similar to C-terminal segment of p60 proteins of Listeria spp.e.g. Q01836|P60_LISIN protein P60 precursor (481 aa), FASTA scores: opt: 241, E():4e-07, (37.7% identity in 122 aa overlap). Highly similar to C-terminal domain of preceeding ORF Rv1477|MTV007.24 (472 aa), FASTA scores: opt: 864, E(): 0, (60.1% identity in 213 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). P9WHU5 No virulence, detoxification, adaptation 3PBI O53169 P9WHU5 P9WHU5 NC_000962.3 Mycobrowser_v5 CDS 1670413 1671366 . + 0 Rv1480 Rv1480 Function unknown Conserved protein Rv1480, (MTV007.27,MTCY227.01), len: 317 aa. Conserved protein, last 110 aa residues correspond to first 110 aa of YS01_MYCAV|O07394 hypothetical 18.7 kDa Mycobacterium avium protein MAV169 (169 aa), FASTA scores: opt: 642, E(): 0, (84.2% identity in 114 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins Rv3163c and Rv3693. P9WLX5 No conserved hypotheticals P64853 P9WLX5 P9WLX5 NC_000962.3 Mycobrowser_v5 CDS 1673440 1674183 . + 0 Rv1483 fabG1 Involved in the fatty acid biosynthesis pathway (first reduction step) (mycolic acid biosynthesis); reduces KASA/KASB products [catalytic activity: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH]. 3-oxoacyl-[acyl-carrier protein] reductase FabG1 (3-ketoacyl-acyl carrier protein reductase) (mycolic acid biosynthesis a protein) Rv1483, (MTCY277.04), len: 247 aa. FabG1 (alternate gene name: mabA), 3-oxoacyl-[acyl-carrier protein] reductase (see citations below), equivalent to O07399|FABG_MYCAV 3-oxoacyl-[acyl-carrier protein] reductase from Mycobacterium avium (255 aa); P71534|FABG_MYCSM 3-oxoacyl-[acyl-carrier protein] reductase from Mycobacterium smegmatis (255 aa); and NP_302228.1|NC_002677 3-oxoacyl-[ACP] reductase (aka MabA) from Mycobacterium leprae (253 aa). Also highly similar to many e.g. T36779 probable 3-oxacyl-(acyl-carrier-protein) reductase from Streptomyces coelicolor (234 aa); FABG_ECOLI|P25716|NP_415611.1|NC_000913 3-oxoacyl-[acyl-carrier-protein] reductase from Escherichia coli strain K12 (244 aa), FASTA scores: opt: 664, E(): 6.8e-35, (44.4% identity in 241 aa overlap); etc. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. P9WGT3 No lipid metabolism 1UZL,1UZM,1UZN,2NTN P0A5Y4 P9WGT3 GO:0051287,GO:0006633,GO:0055114,GO:0004316 1.1.1.100 ETH,INH P9WGT3 NC_000962.3 Mycobrowser_v5 CDS 1675017 1676051 . + 0 Rv1485 hemZ Involved in protoheme biosynthesis (last step). Catalyzes the insertion of ferrous iron into protoporphyrin IX to form protoheme [catalytic activity: protoporphyrin + FE(2+) = protoheme + 2 H(+)]. Ferrochelatase HemZ (protoheme ferro-lyase) (heme synthetase) Rv1485, (MTCY277.06), len: 344 aa. HemZ, ferrochelatase (see citation below), similar to many e.g. HEMZ_BACSU|P32396 ferrochelatase from Bacillus subtilus (310 aa), FASTA scores: opt:490, E(): 2e-24, (30.2% identity in 295 aa overlap); etc. Belongs to the ferrochelatase family. P9WNE3 No intermediary metabolism and respiration P0A576 P9WNE3 GO:0005737,GO:0004325,GO:0006783,GO:0046872,GO:0051537 4.99.1.1 P9WNE3 NC_000962.3 Mycobrowser_v5 CDS 1676017 1676883 . - 0 Rv1486c Rv1486c Function unknown Conserved hypothetical protein Rv1486c, (MTCY277.07c), len: 288 aa. Conserved hypothetical protein, highly similar to YS07_MYCAV|O07402 hypothetical 33.5 kDa protein mav321 from Mycobacterium avium (320 aa), FASTA scores: opt: 1217, E(): 0, (71.1% identity in 315 aa overlap). Weak similarity to AL079332|SCI5.07 hypothetical protein from Streptomyces coelicolor (259 aa), FASTA scores: opt: 131, E(): 0.29, (32.3% identity in 279 aa overlap). Start changed since original submission. P9WLX3 No conserved hypotheticals P9WLX3 NC_000962.3 Mycobrowser_v5 CDS 1690134 1690346 . - 0 Rv1498A Rv1498A Function unknown Conserved protein Rv1498A, len: 70 aa. Conserved protein, highly similar to other hypothetical proteins e.g. from Streptomyces coelicolor, Sinorhizobium meliloti and Pseudomonas aeruginosa. I6XY36 No conserved hypotheticals 3OQT,2YIZ,2YJ0 Q8VK10 I6XY36 I6XY36 NC_000962.3 Mycobrowser_v5 CDS 2176550 2176930 . - 0 Rv1924c Rv1924c Unknown Unknown protein Rv1924c, (MTCY09F9.40), len: 126 aa. Unknown protein. P95289 No conserved hypotheticals P95289 NC_000962.3 Mycobrowser_v5 CDS 1684005 1686257 . + 0 Rv1493 mutB Involved in propionic acid fermentation. Catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates [catalytic activity : (R)-2-methyl-3-oxopropanoyl-CoA = succinyl- CoA.] Probable methylmalonyl-CoA mutase large subunit MutB (MCM) Rv1493, (MTCY277.15), len: 750 aa. Probable mutB, Methylmalonyl-CoA mutase large-subunit, strong similarity to e.g. MUTB_STRCM|Q05065 methylmalonyl-CoA mutase alpha-subunit from Streptomyces cinnamonensis (733 aa), FASTA scores: opt: 3562, E(): 0, (75.8% identity in 730 aa overlap). Contains PS00544 Methylmalonyl-CoA mutase signature. Belongs to the methylmalonyl-CoA mutase family. P9WJK5 No lipid metabolism 1SE5 P65487 P9WJK5 GO:0008152,GO:0046872,GO:0004494,GO:0031419 5.4.99.2 P9WJK5 NC_000962.3 Mycobrowser_v5 CDS 1686271 1686573 . + 0 Rv1494 mazE4 Unknown Possible antitoxin MazE4 Rv1494, (MTCY277.16), len: 100 aa. Possible mazE4, antitoxin, part of toxin-antitoxin (TA) operon with Rv1495 (See Pandey and Gerdes, 2005; Zhu et al., 2006). P9WJ91 No virulence, detoxification, adaptation P9WJ91 P9WJ91 NC_000962.3 Mycobrowser_v5 CDS 1686884 1687888 . + 0 Rv1496 Rv1496 Possibly involved in transport (possibly arginine) Possible transport system kinase Rv1496, (MTCY277.18), len: 334 aa. Possible transport system kinase. Equivalent to NP_302220.1|NC_002677 putative kinase from Mycobacterium leprae (327 aa). Highly similar to several transport system kinases and NTPase transporters e.g. P27254|ARGK_ECOLI|B2918 LAO/AO transport system kinase from Escherichia coli K12 (331 aa) (see citation below); NP_311815.1|NC_002695 ATPase component of two convergent arginine transporter from Escherichia coli O157:H7 (331 aa); etc. Also similar to YPLE_CAUCR|P37895 hypothetical 34.6 kDa protein in Caulobacter crescentus (326 aa), FASTA scores, opt: 1125, E(): 0, (55.7% identity in 316 aa overlap). P9WPZ1 No cell wall and cell processes 3MD0,3P32 P63577 P9WPZ1 GO:0005524 3.6.-.- P9WPZ1 NC_000962.3 Mycobrowser_v5 CDS 1687941 1689230 . + 0 Rv1497 lipL Function unknown, but supposed involvement in lipid metabolism Probable esterase LipL Rv1497, (MTCY277.19), len: 429 aa. Probable LipL, esterase, very similar to Mycobacterium tuberculosis hypothetical esterases and penicillin binding proteins e.g. Rv1923, Rv2463, Rv3775, etc. Also similar to G151214|M68491 esterase estA from Pseudomonas sp (389 aa), FASTA scores: opt: 604, E(): 1e-31, (34.4% identity in 389 aa overlap). P71778 No intermediary metabolism and respiration P71778 P71778 GO:0016787 3.1.-.- P71778 NC_000962.3 Mycobrowser_v5 CDS 1689303 1689920 . - 0 Rv1498c Rv1498c Causes methylation Probable methyltransferase Rv1498c, (MTCY277.20c), len: 205 aa. Probable methyltransferase. Similar to G2792343|AF040571 methyltransferase from amycolatopsis mediterranei (272 aa), FASTA scores: E(): 5.1e-11, (32.3% identity in 124 aa overlap). Contains PS00017 ATP/GTP-binding site motif A. P9WLW9 No intermediary metabolism and respiration P71779 P9WLW9 GO:0008168,GO:0008152 P9WLW9 NC_000962.3 Mycobrowser_v5 CDS 1791570 1792235 . + 0 Rv1591 Rv1591 Function unknown Probable transmembrane protein Rv1591, (MTCY336.13c), len: 221 aa. Probable transmembrane protein, similar to Q49626|LEPB1170_C3_229|YF91_MYCLE Hypothetical Mycobacterium leprae protein (198 aa), FASTA results: opt: 802, E(): 0, (63.8% identity in 188 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WLT5 No cell wall and cell processes P0A5F3 P9WLT5 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1691890 1692711 . + 0 Rv1501 Rv1501 Function unknown Conserved hypothetical protein Rv1501, (MTCY277.23), len: 273 aa. Conserved hypothetical protein, some similarity to O06374|Rv3633|MTCY15C10.19C hypothetical protein from Mycobacterium tuberculosis, FASTA scores: E(): 3.9e-10, (27.5% identity in 280 aa overlap). P9WI91 No conserved hypotheticals P67770 P9WI91 P9WI91 NC_000962.3 Mycobrowser_v5 CDS 1692924 1693823 . + 0 Rv1502 Rv1502 Unknown Hypothetical protein Rv1502, (MTCY277.24), len: 299 aa. Hypothetical unknown protein. P9WLW7 No unknown P9WLW7 P9WLW7 NC_000962.3 Mycobrowser_v5 CDS 1693996 1694544 . - 0 Rv1503c Rv1503c Function unknown Conserved hypothetical protein Rv1503c, (MTCY277.25c), len: 182 aa. Conserved hypothetical protein, similar to C-terminal region of P27833|RFFA_ECOLI lipopolysaccharide biosynthesis protein from Escherichia coli (376 aa), FASTA scores: opt: 565, E(): 0, (49.4% identity in 170 aa overlap); Rv1503c and Rv1504c are both similar to RFFA_ECOLI but are separated by a stop codon, sequence appears to be correct so possible pseudogene. L0T8G4 No conserved hypotheticals Q79FN3 L0T8G4 GO:0030170,GO:0003824 L0T8G4 NC_000962.3 Mycobrowser_v5 CDS 1694545 1695144 . - 0 Rv1504c Rv1504c Function unknown Conserved hypothetical protein Rv1504c, (MTCY277.26c), len: 199 aa. Conserved hypothetical protein, similar to N-terminal region of P27833|RFFA_ECOLI lipopolysaccharide biosynthesis protein from Escherichia coli (376 aa), FASTA scores: opt: 863, E(): 0, (68.0% identity in 194 aa overlap); Rv1503c and Rv1504c are similar to RFFA_ECOLI but are separated by a stop codon, sequence appears to be correct so possible pseudogene. L0T6V0 No conserved hypotheticals Q79FN2 L0T6V0 GO:0030170,GO:0003824 L0T6V0 NC_000962.3 Mycobrowser_v5 CDS 1698095 1699894 . - 0 Rv1508c Rv1508c Unknown Probable membrane protein Rv1508c, (MTCY277.30c), len: 599 aa. Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). Probable membrane protein. P71787 No cell wall and cell processes P71787 P71787 P71787 NC_000962.3 Mycobrowser_v5 CDS 1733610 1734737 . + 0 Rv1533 Rv1533 Unknown Conserved protein Rv1533, (MTCY07A7A.02), len: 375 aa. Conserved protein. Similar to 2NPD_NEUCR|Q01284 2-nitropropane dioxygenase precursor (378 aa), fasta scores: opt: 279, E(): 9.1e-11, (31.3% identity in 256 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins Rv1894c, Rv0021c, Rv3553, Rv2781c. O06179 No conserved hypotheticals O06179 O06179 GO:0004497,GO:0055114,GO:0018580 1.13.12.- O06179 NC_000962.3 Mycobrowser_v5 CDS 2218052 2218717 . + 0 Rv1975 Rv1975 Unknown Conserved hypothetical protein Rv1975, (MTV051.13), len: 221 aa. Conserved hypothetical protein, showing some similarity to AJ251435 hypothetical protein from Mycobacterium avium subsp. paratuberculosis (193 aa). Predicted to be an outer membrane protein (See Song et al., 2008). O53976 No conserved hypotheticals O53976 O53976 NC_000962.3 Mycobrowser_v5 CDS 4095324 4095530 . - 0 Rv3656c Rv3656c Function unknown Conserved hypothetical protein Rv3656c, (MTV025.004c), len: 68 aa. Conserved hypothetical protein, similar to Q9X918|SCH5.16c small hypothetical protein from Streptomyces coelicolor (75 aa), FASTA scores: opt: 129, E(): 0.0039, (40.0% identity in 60 aa overlap). Equivalent to AAK48120 from Mycobacterium tuberculosis strain CDC1551 (42 aa) but longer 26 aa. O69624 No conserved hypotheticals O69624 NC_000962.3 Mycobrowser_v5 CDS 1705058 1705789 . + 0 Rv1513 Rv1513 Function unknown Conserved protein Rv1513, (MTCY277.35), len: 243 aa. Conserved protein, similar to hypothetical proteins from several organisms e.g. AJ223833|MAP223833_3 from Mycobacterium avium paratuberculosis (240 aa), FASTA scores: opt: 1053 E(): 0, (66.3% identity in 243 aa overlap); P74191|SLL1173 from Synechocystis (244 aa), FASTA scores: opt: 276, E(): 1.1e-07, (32.2 % identity in 202 aa overlap). Also highly similar to P95136|Q50460|MTCY349.33c|Rv2956 from Mycobacterium tuberculosis (243 aa), (70.0% identity in 237 aa overlap). P71792 No conserved hypotheticals P71792 P71792 NC_000962.3 Mycobrowser_v5 CDS 1705807 1706595 . - 0 Rv1514c Rv1514c Unknown Conserved hypothetical protein Rv1514c, (MTCY277.36c), len: 262 aa. Conserved hypothetical protein. Similar to other hypothetical proteins, and to WCAE_ECOLI|P71239 putative colanic acid biosynthesis glycosyl transferase (248 aa), FASTA scores: opt: 231, E(): 4.1e-08, (33.3% identity in 210 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical glycosyltransferase, Rv2957. P9WMX9 No conserved hypotheticals P71793 P9WMX9 GO:0016757 2.4.-.- P9WMX9 NC_000962.3 Mycobrowser_v5 CDS 1706630 1707526 . - 0 Rv1515c Rv1515c Function unknown Conserved hypothetical protein Rv1515c, (MTCY277.37c), len: 298 aa. Conserved hypothetical protein, similar to P71805|MTCY02B12.11C|Rv1377c Hypothetical protein from Mycobacterium tuberculosis, FASTA scores: E(): 1.3e-05, (25.4% identity in 134 aa overlap). P71794 No conserved hypotheticals P71794 P71794 GO:0008168,GO:0008152 P71794 NC_000962.3 Mycobrowser_v5 CDS 1707529 1708539 . - 0 Rv1516c Rv1516c Unknown; involved in cellular metabolism. Probable sugar transferase Rv1516c, (MTCY277.38c), len: 336 aa. Probable sugar transferase, similar to AB010970|AB010970_6 glycosyltransferase from Streptococcus mutans (465 aa), FASTA scores: opt: 388, E(): 4.1e-18, (32.7% identity in 214 aa overlap), slight similarity to SPSA_BACSU|P39621 spore coat polysaccharide biosynthesis (256 aa), fasta scores: opt: 185, E(): 6.5e-05, (26.2% identity in 187 aa overlap), strong similarity to Rv1520|MTCY19G5.08c probable sugar transferase from Mycobacterium tuberculosis (63.5% identity in 318 aa overlap). P71795 No intermediary metabolism and respiration P71795 P71795 GO:0016740 2.-.-.- P71795 NC_000962.3 Mycobrowser_v5 CDS 1708871 1709635 . + 0 Rv1517 Rv1517 Unknown Conserved hypothetical transmembrane protein Rv1517, (MTCY277.39), len: 254 aa. Conserved hypothetical transmembrane protein, similar to G466802|LEPB1170_F2_64 from Mycobacterium leprae (230 aa), FASTA scores: opt: 282, E(): 2.2e-11, (34.1% identity in 255 aa overlap). Also similar to Mycobacterium tuberculosis Rv3821|MTCY409.09c (237 aa) (36.3% identity in 256 aa overlap); and Rv3481c. P71796 No cell wall and cell processes P71796 P71796 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1709644 1710603 . + 0 Rv1518 Rv1518 Unknown, possibly glycosyl transferase Conserved hypothetical protein Rv1518, (MTCY277.40, MTCY19G5.11c), len: 319 aa. Conserved hypothetical protein, possibly glycosyl transferase involved in exopolysaccharide synthesis, similar to several hypothetical proteins and glycosyl transferases from diverse organisms e.g. P73996|D90911 from synecho cystis sp. (309 aa), Fasta scores: opt: 300, E(): 1.8e-13, (29.5% identity in 241 aa overlap). P9WLV9 No conserved hypotheticals Q50590 P9WLV9 GO:0016021 P9WLV9 NC_000962.3 Mycobrowser_v5 CDS 1710733 1711002 . + 0 Rv1519 Rv1519 Function unknown Conserved hypothetical protein Rv1519, (MTCY19G5.09c), len: 89 aa. Conserved hypothetical protein, high similarity to C-terminus of Q50723|MTCY78.26|Rv3402c (412 aa) (58.1% identity in 74 aa overlap). P9WLV7 No conserved hypotheticals P64861 P9WLV7 NC_000962.3 Mycobrowser_v5 CDS 1711028 1712068 . + 0 Rv1520 Rv1520 Unknown; thought to be involved in cellular metabolism. Probable sugar transferase Rv1520, (MTCY19G5.08c), len: 346 aa. Probable sugar transferase, similar to several e.g. AB010970|AB010970_6 Streptococcus mutans glycosyltransferase (465 aa), FASTA scores: opt: 381, E(): 1.2e-18, (31.7% identity in 240 aa overlap); O34234|Y07786 sugar transferase from Vibrio cholerae (337 aa), FASTA scores: opt: 214, E(): 8.4e-05, (25.9% identity in 212 aa overlap). Also strongly similar to Mycobacterium tuberculosis probable sugar transferase Rv1516c. Alternative nucleotide at position 1711627 (C->T; Y200Y) has been observed. P9WLV5 No intermediary metabolism and respiration P64863 P9WLV5 NC_000962.3 Mycobrowser_v5 CDS 1717653 1718696 . + 0 Rv1523 Rv1523 Causes methylation Probable methyltransferase Rv1523, (MTCY19G5.05c), len: 347 aa (start uncertain). Probable methyltransferase, similar to G560513|U0002O Mycobacterium leprae (270 aa), FASTA scores: opt: 965, E(): 0, (60.3% identity in 247 aa overlap). Also similar to many e.g. Q54303|X86780 methyltransferase RAPM from Streptomyces hygroscopicus (317 aa), FASTA scores: opt: 323, E(): 1e-15, (41.2% identity in 136 aa overlap). And similar to M. tuberculosis hypothetical proteins Rv2952, Rv1405c, Rv1403c, Rv0839. Q50584 No intermediary metabolism and respiration Q50584 Q50584 GO:0008168,GO:0008152 2.1.1.- Q50584 NC_000962.3 Mycobrowser_v5 CDS 1718726 1719970 . + 0 Rv1524 Rv1524 Unknown Probable glycosyltransferase Rv1524, (MTCY19G5.04c), len: 414 aa. Probable glycosyltransferase, similar to many e.g. P96559|U84349 glycosyltransferase GTFB from Amycolatopsis orientalis (407 aa), FASTA scores: opt: 363, E(): 6.2e-23, (28.8% identity in 430 aa overlap); also high similarity to Rv1526c|MTCY19G5.02 Mycobacterium tuberculosis hypothetical protein (58.7% identity in 416 aa overlap); and AF143772|AF143772_15 glycosyltransferase gtfB from Mycobacterium avium strain 215 (418 aa), FASTA scores: opt: 1801, E(): 0, (65.2% identity in 417 aa overlap). P9WN07 No intermediary metabolism and respiration P64865 P9WN07 GO:0030259,GO:0016758,GO:0005975 2.4.-.- P9WN07 NC_000962.3 Mycobrowser_v5 CDS 1720017 1720802 . + 0 Rv1525 wbbL2 Possibly involved in cell wall arabinogalactan linker formation: Uses dTDP-L-rhamnose as substrate to insert the rhamnosyl residue into the cell wall. Possible rhamnosyl transferase WbbL2 Rv1525, (MT1576, MTCY19G5.03c), len: 261 aa. Possible wbbL2, rhamnosyl transferase (see citation below), showing weak similarity to several rhamnosyl transferases. Similar to AF105060|AF105060_1 Riftia pachyptila endosymbiont (746 aa), FASTA scores: opt: 183, E(): 0.00013, (35.2% identity in 105 aa overlap). P9WLV3 No conserved hypotheticals P9WLV3 P9WLV3 NC_000962.3 Mycobrowser_v5 CDS 1731373 1732476 . + 0 Rv1530 adh Catalyzes the reversible oxidation of ethanol to acetaldehyde with the concomitant reduction of NAD Probable alcohol dehydrogenase Adh Rv1530, (MTV045.04), len: 367 aa. Probable adh, alcohol dehydrogenase, zinc-dependent, similar to many e.g. AE0009|AE000958_23 Archaeoglobus fulgidus section 1 (402 aa), FASTA scores: opt: 423, E(): 1.8e-19, (31.7% identity in 341 aa overlap). Contains PS00059 Zinc-containing alcohol dehydrogenases signature. P9WQC3 No intermediary metabolism and respiration O53904 P9WQC3 GO:0055114,GO:0008270,GO:0004022 1.1.1.1 P9WQC3 NC_000962.3 Mycobrowser_v5 CDS 2218844 2219251 . - 0 Rv1976c Rv1976c Function unknown Conserved hypothetical protein Rv1976c, (MTV051.14), len: 135 aa. Conserved hypothetical protein, similar to SC1C3.03c|AL023702 hypothetical protein from Streptomyces coelicolor (125 aa), FASTA score: opt: 223, E(): 3.3e-08, (39.6% identity in 111 aa overlap). O53977 No conserved hypotheticals O53977 O53977 O53977 NC_000962.3 Mycobrowser_v5 CDS 1722083 1728409 . - 0 Rv1527c pks5 Involved in polyketide metabolism. Probable polyketide synthase Pks5 Rv1527c, (MTV045.01c-MTCY19G5.01), len: 2108 aa. Probable pks5, polyketide synthase, highly similar to many e.g. MCAS_MYCBO|Q02251 mycocerosic acid synthase from Mycobacterium bovis (2110 aa), FASTA scores: opt: 6270, E(): 0, (63.6% identity in 2126 aa overlap). O53901 No lipid metabolism O53901 O53901 GO:0009058,GO:0048037,GO:0055114,GO:0016491,GO:0016740,GO:0008270,GO:0000036 O53901 NC_000962.3 Mycobrowser_v5 CDS 1728953 1729450 . - 0 Rv1528c papA4 Function unknown; thought to be involved in lipid metabolism. Probable conserved polyketide synthase associated protein PapA4 Rv1528c, (MTV045.02), len: 165 aa. Probable papA4, conserved polyketide synthase (PKS) associated protein; shows some similarity to C-terminal part of hypothetical proteins from Mycobacterium tuberculosis and Mycobacterium leprae e.g. Z97188|MTCY409_10 Mycobacterium tuberculosis cosmid (468) (37.9% identity in 66 aa overlap); or U00010_11 Mycobacterium leprae cosmid B1170 (35.7% identity in 84 aa overlap). Also similar to Mycobacterium tuberculosis PKS-associated proteins Rv1182, Rv3824c, Rv3820c. O53902 No lipid metabolism O53902 NC_000962.3 Mycobrowser_v5 CDS 1729502 1731256 . + 0 Rv1529 fadD24 Function unknown, but involvement in lipid degradation. Probable fatty-acid-AMP ligase FadD24 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) Rv1529, (MTV045.03), len: 584 aa. Probable fadD24, fatty-acid-AMP synthetase, highly similar to many e.g. MBU75685_1|AAB52538.1|U75685 acyl-CoA synthase from Mycobacterium bovis (582 aa), FASTA score: (65.6% identity in 582 aa overlap); and many other fatty-acid-CoA synthetases from Mycobacteria e.g. fadD25|MTCY19G5_7 from Mycobacterium tuberculosis (583 aa), FASTA score: (68.7% identity in 584 aa overlap); fadD28|MTCY24G1_8 from Mycobacterium tuberculosis (580 aa), FASTA score: (66.0% identity in 582 aa overlap); NP_301232.1|NC_002677|U00010_6 from Mycobacterium leprae (372 aa), FASTA score: (57.6% identity in 342 aa overlap); FADD23|Rv3826|MTCY409.04c from Mycobacterium tuberculosis (584 aa), FASTA score: (63.2% identity in 584 aa overlap); etc. O53903 No lipid metabolism O53903 O53903 GO:0008152,GO:0016874 6.2.1.- O53903 NC_000962.3 Mycobrowser_v5 CDS 1732473 1733039 . + 0 Rv1531 Rv1531 Unknown Conserved protein Rv1531, (MTV045.05), len: 188 aa. Conserved protein, similar to Rv0464c|MTV038.08c (190 aa), FASTA scores: E(): 4.8e-10, (30.9% identity in 175 aa overlap). O53905 No conserved hypotheticals O53905 O53905 O53905 NC_000962.3 Mycobrowser_v5 CDS 1734734 1735411 . + 0 Rv1534 Rv1534 Possibly involved in a transcriptional mechanism Probable transcriptional regulator Rv1534, (MTCY07A7A.03), len: 225 aa. Probable transcriptional regulator, similar to YCDC_ECOLI|P75899 hypothetical transcriptional regulator from Escherichia coli (212 aa), FASTA scores: opt: 166, E(): 9.8e-05, (24.2% identity in 219 aa overlap). Contains PS01081 Bacterial regulatory proteins, TetR family signature and helix turn helix motif (aa 41-62). O08377 No regulatory proteins O08377 O08377 GO:0006350,GO:0003700,GO:0006355 O08377 NC_000962.3 Mycobrowser_v5 CDS 1736519 1739644 . + 0 Rv1536 ileS Charging ILE tRNA [catalytic activity: ATP + L-isoleucine + tRNA(ILE) = AMP + diphosphate + L-isoleucyl-tRNA(ILE)]. Isoleucyl-tRNA synthetase IleS Rv1536, (MTCY48.29c-MTCCY07A7A.05), len: 1041 aa. ileS, Isoleucyl-tRNA synthetase , similar to several e.g. SYIC_YEAST P09436 isoleucyl-tRNA synthetase (1072 aa), FASTA scores: opt: 1447, E(): 0, (37.8% identity in 1072 aa overlap); contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Belongs to class-I aminoacyl-tRNA synthetase family. P9WFV3 No information pathways Q10765 P9WFV3 GO:0005737,GO:0004822,GO:0006428,GO:0046677,GO:0008270,GO:0005524 6.1.1.5 P9WFV3 NC_000962.3 Mycobrowser_v5 CDS 1741212 1742192 . - 0 Rv1538c ansA Conversion of asparagine to aspartate [catalytic activity: L-asparagine + H(2)O = L-aspartate + NH(3).] Probable L-aparaginase AnsA Rv1538c, (MTCY48.27), len: 326 aa. Probable ansA, L-aparaginase, most similar to ASPG_BACLI|P30363 L-asparaginase (322 aa), FASTA scores: opt: 417, E(): 8.8e-19, (30.9% identity in 314 aa overlap). Contains PS00917 Asparaginase / glutaminase active site signature 2. P9WPX5 No intermediary metabolism and respiration P63627 P9WPX5 GO:0006520,GO:0005737,GO:0004067 3.5.1.1 P9WPX5 NC_000962.3 Mycobrowser_v5 CDS 1742244 1742852 . + 0 Rv1539 lspA This protein specifically catalyzes the removal of signal peptides from prolipoproteins [catalytic activity: cleavage of N-terminal leader sequences from membrane prolipoproteins. Hydrolyses XAA-XBB-XBB-|-CYS, in which XAA is hydrophobic (preferably LEU), XBB is often SER or ala, XCC is often GLY or ala, and the CYS is alkylated on sulfur with a diacylglyceryl group]. Probable lipoprotein signal peptidase LspA Rv1539, (MTCY48.26c), len: 202 aa. Probable lspA, lipoprotein signal peptidase (see citation below), similar to several e.g. LSPA_PSEFL|P17942 (170 aa), FASTA scores: opt: 299, E(): 2.6e-12, (38.3% identity in 167 aa overlap). Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P9WK99 No cell wall and cell processes P65262 P9WK99 GO:0016021,GO:0005886,GO:0006508,GO:0004190 3.4.23.36 P9WK99 NC_000962.3 Mycobrowser_v5 CDS 1742845 1743771 . + 0 Rv1540 Rv1540 Function unknown Conserved hypothetical protein member of yabO/yceC/yfiI family Rv1540, (MTCY48.25c), len: 308 aa. Member of the yabO/yceC/yfiI family of hypothetical proteins, similar to P44445|YFII_HAEIN hypothetical protein HI0176 from Haemophilus influenzae (324 aa), FASTA scores: opt: 437, E(): 1.2e-22, (33.2% identity in 322 aa overlap). Equivalent to AL049478|MLCL458_13 hypothetical protein from Mycobacterium leprae (308 aa), (89.3% identity in 307 aa overlap). Contains PS01129 hypothetical yabO/yceC/yfiI family signature. P9WHQ3 No conserved hypotheticals P0A5T2 P9WHQ3 GO:0009982,GO:0001522,GO:0003723 5.4.99.- P9WHQ3 NC_000962.3 Mycobrowser_v5 CDS 1745064 1746089 . + 0 Rv1543 Rv1543 Thought to reduce acyl-CoA esters of fatty acids to fatty aldehydes. Possible fatty acyl-CoA reductase Rv1543, (MTCY48.22c), len: 341 aa. Possible fatty-acyl CoA reductase, highly similar to P94129|U77680 fatty acyl-CoA reductase ACR1 from Acinetobacter calcoaceticus (295 aa), FASTA scores: opt: 899, E(): 0, (48.5% identity in 293 aa overlap). Also highly similar to acrA1|Rv3391|MTV004.49|NP_217908.1|NC_000962 fatty acyl-CoA reductase from Mycobacterium tuberculosis (650 aa). Also highly similar to many oxidoreductases short-chain family. P9WGS1 No lipid metabolism P66779 P9WGS1 GO:0055114,GO:0016491 1.-.-.- P9WGS1 NC_000962.3 Mycobrowser_v5 CDS 1746094 1746897 . + 0 Rv1544 Rv1544 Function unknown, but possibly involvement in lipid metabolism. Possible ketoacyl reductase Rv1544, (MTCY48.21), len: 267 aa. Possible ketoacyl reductase, highly similar to Z97179|MLCL383_26 putative oxidoreductase from Mycobacterium leprae (268 aa), FASTA score: (43.0% identity in 270 aa overlap). Also highly similar to others e.g. T29125 ketoacyl reductase homolog from Streptomyces coelicolor (276 aa); NP_470957.1|NC_003212 protein similar to ketoacyl reductases from Listeria innocua (253 aa); HETN_ANASP|P37694 ketoacyl reductase from Anabaena sp. strain PCC 7120 (287 aa), FASTA scores: opt: 379, E(): 7.5e-18, (31.6% identity in 250 aa overlap); etc. And highly similar to many oxidoreductases short-chain family. Also highly similar to Rv2509 from Mycobacterium tuberculosis (268 aa). Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. Q10782 No lipid metabolism Q10782 Q10782 GO:0055114,GO:0016491 1.3.1.- Q10782 NC_000962.3 Mycobrowser_v5 CDS 1746919 1747146 . + 0 Rv1545 Rv1545 Unknown Hypothetical protein Rv1545, (MTCY48.20), len: 75 aa. Hypothetical unknown protein. P9WLU9 No conserved hypotheticals P9WLU9 NC_000962.3 Mycobrowser_v5 CDS 1780199 1780699 . + 0 Rv1575 Rv1575 Unknown Probable PhiRv1 phage protein Rv1575, (MTCY336.29c), len: 166 aa. Probable phiRV1 phage protein (see citation below), showing similarity in N-terminal part to Rv1574|MTCY336.30c Probable phiRV1 phage protein (103 aa), FASTA score: opt: 375, E(): 3.8e-16, (60.2% identity in 103 aa overlap); and Rv2647 Probable phiRV2 phage protein. Start changed since first submission (+49 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L0T9U5 No insertion seqs and phages L0T9U5 NC_000962.3 Mycobrowser_v5 CDS 1755445 1757310 . + 0 Rv1551 plsB1 Thought to be involved in lipid metabolism. Possible acyltransferase PlsB1 Rv1551, (MT1601, MTCY48.14c), len: 621 aa. Possible plsB1, acyltransferase, similar to PLSB_HAEIN|P44857 glycerol-3-phosphate acyltransferase from Haemophilus influenzae (810 aa), FASTA scores: opt: 434, E(): 6.2e-22, (27.6% identity in 395 aa overlap). Also similar to Rv2482c|plsB2 Probable glycerol-3-phosphate acyltransferase from Mycobacterium tuberculosis (789 aa). P9WI59 No lipid metabolism P65734 P9WI59 GO:0008654,GO:0005886,GO:0004366 P9WI59 NC_000962.3 Mycobrowser_v5 CDS 1747694 1751248 . + 0 Rv1547 dnaE1 DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase [catalytic activity: N deoxynucleoside triphosphate = N diphosphate + {DNA}(N)]. Probable DNA polymerase III (alpha chain) DnaE1 (DNA nucleotidyltransferase) Rv1547, (MTCY48.18c), len: 1184 aa. Probable dnaE1, DNA polymerase III, alpha chain (see citation below), similar to many e.g. DP3A_ECOLI|P10443 dna polymerase III, alpha chain (1160 aa), FASTA scores: opt: 1789, E(): 0, (36.5% identity in 1193 aa overlap). Also similar to M. tuberculosis, DnaE2|Rv3370c. Belongs to DNA polymerase type-C family, DNAE subfamily. P9WNT7 No information pathways P63977 P9WNT7 GO:0003677,GO:0006260,GO:0003887,GO:0005737,GO:0008408 2.7.7.7 P9WNT7 NC_000962.3 Mycobrowser_v5 CDS 1753510 1754037 . + 0 Rv1549 fadD11.1 Function unknown, but involvement in lipid degradation. Possible fatty-acid-CoA ligase FadD11.1 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv1549, (MTCY48.16c), len: 175 aa. Possible fadD11.1, fatty-acid-CoA synthetase, similar to the N-terminus of many fatty-acid CoA synthetases e.g. NP_147860.1|NC_000854 long-chain-fatty-acid--CoA ligase from Aeropyrum pernix (651 aa); P31685|4CL2_SOLTU 4-coumarate--CoA ligase 2 from Solanum tuberosum (Potato) (545 aa), FASTA scores: opt: 168, E(): 4.4e-06, (30.4% identity in 112 aa overlap); etc. Possible frameshift with respect to next ORF Rv1550|MTCY48.15c but we can find no sequence error to account for this. Note that previously known as fadD11'. P9WLU5 No lipid metabolism Q10777 P9WLU5 GO:0008152,GO:0003824 P9WLU5 NC_000962.3 Mycobrowser_v5 CDS 1782072 1782584 . - 0 Rv1577c Rv1577c Unknown Probable PhiRv1 phage protein Rv1577c, (MTCY336.27), len: 170 aa. Probable phiRv1 phage protein (prohead protease) (see citation below). Highly similar to hypothetical protein Rv2651c|MTCY441.20c phiRV2 prohead protease, FASTA scores: E(): 0, (89.3% identity in 169 aa overlap). Some similarity to VP4_BPHK7|P49860 putative bacteriophage HK97 prohead protease (gp4) (225 aa), FASTA results: opt: 176, E(): 1.3e-05, (27.3% identity in 165 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06613 No insertion seqs and phages O06613 O06613 O06613 NC_000962.3 Mycobrowser_v5 CDS 1794756 1795805 . + 0 Rv1594 nadA Quinolinate biosynthesis Probable quinolinate synthetase NadA Rv1594, (MTCY336.10c), len: 349 aa. Probable nadA, quinolinate synthetase. Similar to many e.g. Q49622 NADA from Mycobacterium leprae (368 aa), FASTA results: opt: 1994, E(): 0, (84.4% identity in 352 aa overlap). P9WJK1 No intermediary metabolism and respiration P65497 P9WJK1 GO:0009435,GO:0005737,GO:0046872,GO:0008987,GO:0016740,GO:0051539 2.5.1.72 P9WJK1 NC_000962.3 Mycobrowser_v5 CDS 1759435 1760178 . + 0 Rv1553 frdB Involved in interconversion of fumarate and succinate (anaerobic respiration) [catalytic activity: succinate + acceptor = fumarate + reduced acceptor]. Probable fumarate reductase [iron-sulfur subunit] FrdB (fumarate dehydrogenase) (fumaric hydrogenase) Rv1553, (MTCY48.12c), len: 247 aa. Probable frdB, fumarate reductase, iron-sulfur subunit, highly similar to others e.g. P00364|FRDB_ECOLI fumarate reductase iron-sulfur protein from Escherichia coli strain K12 (243 aa), FASTA scores: opt: 846, E(): 0, (50.0% identity in 242 aa overlap); P20921|FRDB_PROVU fumarate reductase iron-sulfur protein from Proteus vulgaris (245 aa); G64097 fumarate reductase iron-sulfur protein from Haemophilus influenzae (276 aa); etc. Contains PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature. Note that fumarate reductase forms part of an enzyme complex containing four subunits: a flavoprotein (Rv1552|frdA), an iron-sulfur (Rv1553|frdB), and two hydrophobic anchor proteins (Rv1554|frdC and Rv1555|frdD). P9WN89 No intermediary metabolism and respiration P9WN89 GO:0051538,GO:0051539,GO:0009055,GO:0022900,GO:0046872,GO:0000104,GO:0006810,GO:0006099,GO:0051537 1.3.99.1 P9WN89 NC_000962.3 Mycobrowser_v5 CDS 1760175 1760555 . + 0 Rv1554 frdC Involved in interconversion of fumarate and succinate (anaerobic respiration). This hydrophobic component may be required to anchor the catalytic components of the fumarate reductase complex to the cytoplasmic membrane. Probable fumarate reductase [membrane anchor subunit] FrdC (fumarate dehydrogenase) (fumaric hydrogenase) Rv1554, (MTCY48.11c), len: 126 aa. Probable frdC, fumarate reductase, membrane-anchor subunit, highly similar to others e.g. P03805|FRDC_ECOLI fumarate reductase 15 kDa hydrophobic protein from Escherichia coli strain K12 (131 aa), FASTA scores, opt: 268, E(): 3.9e-10, (31.1% identity in 122 aa overlap); NP_458780.1|NC_003198 fumarate reductase complex subunit C; membrane anchor polypeptide from Salmonella enterica subsp. enterica serovar Typhi (131 aa); P20923|FRDC_PROVU fumarate reductase 15 kDa hydrophobic protein from Proteus vulgaris (131 aa); etc. Note that fumarate reductase forms part of an enzyme complex containing four subunits: a flavoprotein (Rv1552|frdA), an iron-sulfur (Rv1553|frdB), and two hydrophobic anchor proteins (Rv1554|frdC and Rv1555|frdD). P9WNB7 No intermediary metabolism and respiration Q10762 P9WNB7 GO:0005886,GO:0016021 P9WNB7 NC_000962.3 Mycobrowser_v5 CDS 1760552 1760929 . + 0 Rv1555 frdD Involved in interconversion of fumarate and succinate (anaerobic respiration). This hydrophobic component may be required to anchor the catalytic components of the fumarate reductase complex to the cytoplasmic membrane. Probable fumarate reductase [membrane anchor subunit] FrdD (fumarate dehydrogenase) (fumaric hydrogenase) Rv1555, (MTCY48.10c), len: 125 aa. Probable frdD, fumarate reductase, membrane-anchor subunit, similar to others e.g. P03806|FRDD_ECOLI fumarate reductase 13 kDa hydrophobic protein from Escherichia coli strain K12 (119 aa), FASTA scores: opt: 212, E(): 4.4e-08, (36.8% identity in 106 aa overlap); etc. Note that fumarate reductase forms part of an enzyme complex containing four subunits: a flavoprotein (Rv1552|frdA), an iron-sulfur (Rv1553|frdB), and two hydrophobic anchor proteins (Rv1554|frdC and Rv1555|frdD). P9WNB5 No intermediary metabolism and respiration P67643 P9WNB5 GO:0016021,GO:0005886,GO:0006106 P9WNB5 NC_000962.3 Mycobrowser_v5 CDS 1782758 1783228 . - 0 Rv1578c Rv1578c Unknown Probable PhiRv1 phage protein Rv1578c, (MTCY336.26), len: 156 aa. Probable phiRv1 phage protein (terminase) (see citation below), highly similar to Rv2652c|MTCY441.21c phiRV2 phage protein from Mycobacterium tuberculosis, FASTA scores: E(): 4.8e-22, (48.1% identity in 156 aa overlap). Also similar to X65555|ARP3COS_1 hypothetical protein (cos site) - actinophage RP3 (210 aa), FASTA scores: opt: 373, E(): 6.5e-17, (50.0% identity in 114 aa overlap). Contains MIP family signature (PS00221). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06612 No insertion seqs and phages O06612 NC_000962.3 Mycobrowser_v5 CDS 1763428 1764717 . + 0 Rv1559 ilvA Involved in isoleucine biosynthesis (first step). Catalyzes the formation of alpha-ketobutyrate from threonine in a two step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia [catalytic activity: L-threonine + H(2)O = 2-oxobutanoate + NH(3) + H(2)O]. Probable threonine dehydratase IlvA Rv1559, (MTCY48.06c), len: 429 aa. Probable ilvA, threonine dehydratase, biosynthetic protein, similar to several e.g. THD1_CORGL|Q04513 threonine dehydratase biosynthetic (436 aa), FASTA scores: opt: 1694, E(): 0, (61.9% identity in 415 aa overlap). Contains PS00165 Serine/threonine dehydratases pyridoxal-phosphate attachment site. P9WG95 No intermediary metabolism and respiration P66897 P9WG95 GO:0009097,GO:0030170,GO:0004794 4.3.1.19 P9WG95 NC_000962.3 Mycobrowser_v5 CDS 1767135 1769432 . - 0 Rv1563c treY Involved in trehalose biosynthesis (protective effect). Mycobacteria can produce trehalose from glucose 6-phosphate and UDP-glucose (the OtsA-OtsB pathway) from glycogen-like alpha(1-->4)-linked glucose polymers (the TreY-TreZ pathway) and from maltose (the TreS pathway). Maltooligosyltrehalose synthase TreY Rv1563c, (MTCY48.02), len: 765 aa. TreY (previously called glgY), maltooligosyl trehalose synthase, confirmed biochemically (see citation below). Strong similarity to Q44315|63343 trey maltooligosyl trehalose synthase from arthrobacter SP (775 aa), fasta scores: opt: 1953, E(): 0; (46.0% identity in 789 aa overlap). Some similarity to alpha-amylases and to MTCY48.03 (30.2% identity in 215 aa overlap). May catalyse conversion of maltodextrins to maltooligosyl trehaloses. Also similar to Mycobacterium tuberculosis glgB (Rv1326c), treZ (Rv1562c). P9WQ21 No virulence, detoxification, adaptation Q10768 P9WQ21 GO:0005975,GO:0043169,GO:0047470 5.4.99.15 P9WQ21 NC_000962.3 Mycobrowser_v5 CDS 1773928 1774620 . - 0 Rv1566c Rv1566c Unknown Possible Inv protein Rv1566c, (MTCY336.37), len: 230 aa. Possible inv protein, probably exported as has QQAPV repeats at C-terminus. Similar to Q49634 inv protein from Mycobacterium leprae (246 aa), FASTA scores: opt: 957, E(): 0, (70.0% identity in 207 aa overlap); also to putative invasins 1,2 (O07390, O07391) from Mycobacterium avium. Slightly similar to C-terminus of P60_LISMO|P21171 Listeria invasion-associated protein p60 precursor. Also similar to Mycobacterium tuberculosis p60 homologues Rv1477, Rv1478, Rv0024, Rv2190c. Predicted to be an outer membrane protein (See Song et al., 2008). O06624 No virulence, detoxification, adaptation O06624 O06624 O06624 NC_000962.3 Mycobrowser_v5 CDS 1776702 1777862 . + 0 Rv1569 bioF1 Involved in biotin biosynthesis (at the first step) [catalytic activity: 6-carboxyhexanoyl-CoA + L-alanine = 8-amino-7-oxononanoate + CoA + CO2]. Probable 8-amino-7-oxononanoate synthase BioF1 (AONS) (8-amino-7-ketopelargonate synthase) (7-keto-8-amino-pelargonic acid synthetase) (7-KAP synthetase) (L-alanine--pimelyl CoA ligase) Rv1569, (MTCY336.34c), len: 386 aa. Probable bioF1, 8-amino-7-oxononanoate synthase, highly similar to BIOF_MYCLE|P45487 from Mycobacterium leprae (385 aa), FASTA results: opt: 1971, E(): 0, (80.1% identity in 381 aa overlap). Also similar to BIOF2|Rv0032|MTCY10H4.32 possible 8-amino-7-oxononanoate synthase from Mycobacterium tuberculosis (771 aa), FASTA score: E(): 5.5e-29, (37.4% identity in 393 aa overlap). Contains aminotransferases class-II pyridoxal-phosphate attachment site (PS00599). Belongs to class-II of pyridoxal-phosphate-dependent aminotransferases. P9WQ87 No intermediary metabolism and respiration P0A4X4 P9WQ87 GO:0009102,GO:0008890,GO:0030170,GO:0016769,GO:0008710 2.3.1.29,2.3.1.47 P9WQ87 NC_000962.3 Mycobrowser_v5 CDS 1777859 1778539 . + 0 Rv1570 bioD Involved in bioconversion of pimelate into dethiobiotin [catalytic activity: ATP + 7,8-diaminononanoate + CO(2) = ADP + phosphate + dethiobiotin] Dethiobiotin synthetase BioD Rv1570, (MTCY336.33c), len: 226 aa. bioD, dethiobiotin synthetase. Similar to many e.g. BIOD_MYCLE|P45486 from Mycobacterium leprae (223 aa), FASTA results: opt: 1059, E(): 0, (74.8% identity in 222 aa overlap). Belongs to the dethiobiotin synthetase family. P9WPQ5 No intermediary metabolism and respiration 3FGN,3FMF,3FMI,3FPA P9WPQ5 GO:0009102,GO:0004141,GO:0000287,GO:0005524 6.3.3.3 P9WPQ5 NC_000962.3 Mycobrowser_v5 CDS 1779930 1780241 . + 0 Rv1574 Rv1574 Unknown Probable PhiRv1 phage related protein Rv1574, (MTCY336.30), len: 103 aa. Probable phiRV1 phage related protein (see citation below); some similarity to N-terminus of Rv1575|MTCY441.17 Probable phiRV1 phage protein (166 aa), E(): 1.5e-06; and Rv2647|MTCY336.29c Probable phiRV2 phage protein, E(): 3.5e-05. Helix turn helix motif present at aa 14-35 (+3.61 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L0TA08 No insertion seqs and phages L0TA08 NC_000962.3 Mycobrowser_v5 CDS 1783906 1784301 . - 0 Rv1581c Rv1581c Unknown Probable PhiRv1 phage protein Rv1581c, (MTCY336.23), len: 131 aa. Probable phiRv1 phage protein (see citation below). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06609 No insertion seqs and phages O06609 O06609 NC_000962.3 Mycobrowser_v5 CDS 1785912 1786310 . - 0 Rv1583c Rv1583c Unknown Probable PhiRv1 phage protein Rv1583c, (MTCY336.21), len: 132 aa. Probable phiRv1 phage protein (see citation below), highly similar to Rv2656c|MTCY441.25c phiRV2 phage protein (130 aa), FASTA score: E(): 1.3e-33, (81.7% identity in 131 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLU1 No insertion seqs and phages P9WLU1 NC_000962.3 Mycobrowser_v5 CDS 1786584 1787099 . - 0 Rv1585c Rv1585c Unknown Possible phage PhiRv1 protein Rv1585c, (MTCY336.19), len: 171 aa. Possible phage phiRv1 protein (see Hatfull 2000). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06605 No insertion seqs and phages O06605 NC_000962.3 Mycobrowser_v5 CDS 1788162 1789163 . - 0 Rv1587c Rv1587c Unknown Partial REP13E12 repeat protein Rv1587c, (MTCY336.17), len: 333 aa. Partial REP13E12 repeat protein (see citation below), nearly identical (but has been interrupted by phiRv1 prophage) to Q50655|MTCY251.13c|Rv0094c hypothetical 34.6 kDa protein from M. tuberculosis (317 aa), FASTA results: opt: 1511, E(): 1.1e-84, (97.75% identity in 224 aa overlap). Codon usage suggests that translation may involve frameshifting of Rv1588c mRNA in poly_C stretch into reading frame of Rv1587c. 3' end found in Rv1572c. Length extended since first submission (+115 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06603 No insertion seqs and phages O06603 O06603 O06603 NC_000962.3 Mycobrowser_v5 CDS 1789168 1789836 . - 0 Rv1588c Rv1588c Unknown Partial REP13E12 repeat protein Rv1588c, (MTCY336.16), len: 222 aa. Partial REP13E12 repeat protein (see citation below), nearly identical to ORF's in other Rep13E12 repeats, including Rv0095c|MTCY251.14c|Y05E_MYCTU|Q10891 hypothetical 15.4 kd protein cy251.14 from Mycobacterium tuberculosis (136 aa), FASTA results: opt: 613, E(): 9.9e-29, (86.5% identity in 111 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLT9 No insertion seqs and phages P9WLT9 NC_000962.3 Mycobrowser_v5 CDS 1790284 1791333 . + 0 Rv1589 bioB Involved in biotin synthesis. Probable biotin synthetase BioB Rv1589, (MTCY336.15c), len: 349 aa. Probable bioB, biotin synthetase O06601. Highly similar to BIOB_MYCLE|P46715 BioB from Mycobacterium leprae (345 aa), FASTA results: opt: 1982, E(): 0, (86.5% identity in 349 aa overlap). Identical to AF041819|AF041819_9 bioB from Mycobacterium bovis BCG (349 aa). P9WPQ7 No intermediary metabolism and respiration P0A506 P9WPQ7 GO:0051539,GO:0009102,GO:0004076,GO:0005506,GO:0051537 2.8.1.6 P9WPQ7 NC_000962.3 Mycobrowser_v5 CDS 1792400 1793740 . - 0 Rv1592c Rv1592c Function unknown Conserved hypothetical protein Rv1592c, (MTCY336.12), len: 446 aa. Conserved hypothetical protein, some similarity to Q49629|B1170_F1_46 from Mycobacterium leprae (132 aa), FASTA results: opt: 332, E(): 4.5e-14, (56.3% identity in 87 aa overlap). Nearly identical to truncated Mycobacterium bovis BCG protein (148 aa) AF041819|AF041819_11. P9WK89 No conserved hypotheticals O06598 P9WK89 GO:0004806,GO:0016042 INH P9WK89 NC_000962.3 Mycobrowser_v5 CDS 1797388 1798245 . + 0 Rv1596 nadC de novo biosynthesis of NAD and NADP [catalytic activity: nicotinate D-ribonucleotide + diphosphate + CO(2) = pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate] Probable nicotinate-nucleotide pyrophosphatase NadC Rv1596, (MTCY336.08c), len: 285 aa. Probable nadC, nicotinate-nucleotide pyrophosphatase O06594. Similar to many e.g. ADC_MYCLE|P46714 from Mycobacterium leprae (284 aa), FASTA results: opt: 1418, E(): 0,(79.2% identity in 283 aa overlap). Belongs to the NADC/MODD family. P9WJJ7 No intermediary metabolism and respiration 1QPN,1QPO,1QPQ,1QPR O06594 P9WJJ7 GO:0004514,GO:0009435 2.4.2.19 P9WJJ7 NC_000962.3 Mycobrowser_v5 CDS 1798294 1799052 . + 0 Rv1597 Rv1597 Unknown Hypothetical protein Rv1597, (MTCY336.07c), len: 252 aa. Hypothetical unknown protein. O06593 No conserved hypotheticals O06593 O06593 GO:0008168,GO:0008152 O06593 NC_000962.3 Mycobrowser_v5 CDS 1799073 1799483 . - 0 Rv1598c Rv1598c Function unknown Conserved protein Rv1598c, (MTCY336.06), len: 136 aa. Conserved protein, some similarity to O06389|Rv0523c|MTCY25D10.02 from Mycobacterium tuberculosis (131 aa), FASTA scores: E(): 2.2e-09, (38.4% identity in 99 aa overlap); and P95144|MTCY359.02|Rv1871c (129 aa). O06592 No conserved hypotheticals O06592 O06592 NC_000962.3 Mycobrowser_v5 CDS 1857731 1858537 . + 0 Rv1648 Rv1648 Unknown Probable transmembrane protein Rv1648, (MTCY06H11.13), len: 268 aa. Probable transmembrane protein, some similarity to Rv3434c|MTCY77.06C (237 aa), FASTA scores: E(): 0.00039, (31.4% identity in 194 aa overlap). P94983 No cell wall and cell processes P94983 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1944809 1945627 . + 0 Rv1718 Rv1718 Function unknown Conserved hypothetical protein Rv1718, (MTCY04C12.03), len: 272 aa. Conserved hypothetical protein, similar to O29058|AF1210|AE001021 Hypothetical protein from Archeoglobus (313 aa), FASTA scores: opt: 301, E(): 8e-23, (31.6% identity in 301 aa overlap). P71976 No conserved hypotheticals P71976 P71976 P71976 NC_000962.3 Mycobrowser_v5 CDS 1951041 1951751 . - 0 Rv1725c Rv1725c Function unknown Conserved hypothetical protein Rv1725c, (MTCY04C12.10c), len: 236 aa. Conserved hypothetical protein, similar to other hypothetical proteins from diverse organisms e.g. P70885|U44893 ORF108 from butyrivibrio fibrisolvens, (108 aa), FASTA scores: opt: 223, E(): 2e-09, (39.1% identity in 92 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical transcriptional regulator, O05774|Rv3095|YU95_MYCTU (158 aa). P71983 No conserved hypotheticals P71983 P71983 P71983 NC_000962.3 Mycobrowser_v5 CDS 1799583 1800899 . + 0 Rv1599 hisD Involved in histidine biosynthesis pathway (tenth step). This protein is considered as a bifunctional enzyme, possessing two active sites, one an alcohol dehydrogenase and the other an aldehyde dehydrogenase [catalytic activity: L-histidinol + 2 NAD(+) + H(2)O = L-histidine + 2 NADH]. Probable histidinol dehydrogenase HisD (HDH) Rv1599, (MTCY336.05c), len: 438 aa. Probable hisD, histidinol dehydrogenase (see citation below) O08396. Similar to many e.g. HISX_MYCSM|P28736 from Mycobacterium smegmatis (445 aa), FASTA results: opt: 2356, E(): 0, (83.1% identity in 437 aa overlap). Contains histidinol dehydrogenase signature (PS00611). P9WNW9 No intermediary metabolism and respiration P63950 P9WNW9 GO:0008270,GO:0000105,GO:0004399,GO:0055114,GO:0051287 1.1.1.23 P9WNW9 NC_000962.3 Mycobrowser_v5 CDS 1900241 1900975 . - 0 Rv1675c cmr Involved in transcriptional mechanism Probable transcriptional regulatory protein Cmr Rv1675c, (MTV047.10c), len: 244 aa. Probable cmr, cAMP and macrophage regulator, transcriptional regulatory protein, weak similarity to D00496|LBATRP_7 trp operon from Lactobacillus casei (219 aa), FASTA scores: opt: 172, E(): 0.00011, (26.9% identity in 186 aa overlap). P9WMH5 No regulatory proteins O53922 P9WMH5 GO:0006355,GO:0003700,GO:0005622 P9WMH5 NC_000962.3 Mycobrowser_v5 CDS 1802664 1803284 . + 0 Rv1602 hisH Histidine biosynthesis pathway (fifth step). Catalyzes an amidotransferase reaction that generates imidazole-glycerol phosphate and 5-aminoimidazol-4-carboxamide ribonucleotide, which is used for purine synthesis. Probable amidotransferase HisH Rv1602, (MTCY336.02c), len: 206 aa. Probable hisH, amidotransferase. Similar to many e.g. HIS5_STRCO|P16249 from Streptomyces coelicolor (222 aa), FASTA results: opt: 872, E():0, (61.0% identity in 210 aa overlap). Contains glutamine amidotransferases class-I active site (PS00442). Belongs to the HisH family. P9WMM1 No intermediary metabolism and respiration O06589 P9WMM1 GO:0006541,GO:0000105,GO:0000107,GO:0005737 2.4.2.- P9WMM1 NC_000962.3 Mycobrowser_v5 CDS 1803294 1804031 . + 0 Rv1603 hisA Histidine biosynthesis pathway (fourth step) [catalytic activity: N-(5'-phospho-D-ribosylformimino)-5-amino-1-(5''-phosphoribosyl)-4-imidazolecarb oxamide = N-(5'-phospho-D-1'-ribulosylformimino)-5-amino-1-(5''-phosphoribosyl)-4- imidazolecarboxamide.] Probable phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase HisA Rv1603, (MTV046.01-MTCY336.01c), len: 245 aa. Probable hisA, phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase, similar to many e.g. HIS4_STRCO|P16250 phosphoribosylformimino-5-aminoimidaz from Streptomyces coelicolor (240 aa), FASTA scores: opt: 1081, E(): 0, (69.0% identity in 239 aa overlap). P9WMM5 No intermediary metabolism and respiration 2Y85,2Y88,2Y89 P60578 P9WMM5 GO:0005737,GO:0000105,GO:0004640,GO:0000162,GO:0003949 5.3.1.24,5.3.1.16 P9WMM5 NC_000962.3 Mycobrowser_v5 CDS 1804039 1804851 . + 0 Rv1604 impA Involved in inositol phosphate metabolism. It is responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides. Key enzyme of the phosphatidyl inositol signaling pathway [catalytic activity: inositol 1(or 4)-monophosphate + H(2)O = inositol + orthophosphate]. Probable inositol-monophosphatase ImpA (imp) Rv1604, (MTV046.02), len: 270 aa. Probable impA, inositol monophosphatase, similar to many e.g. AF0059|AF005905_2 inositol monophosphate phosphatase from Mycobacterium smegmatis (276 aa), FASTA scores: opt: 1241, E(): 0, (70.5% identity in 261 aa overlap). Also similar to Mycobacterium tuberculosis proteins Rv3137 and Rv2701c. O53907 No intermediary metabolism and respiration O53907 O53907 GO:0008934 3.1.3.25 O53907 NC_000962.3 Mycobrowser_v5 CDS 1804853 1805656 . + 0 Rv1605 hisF Histidine biosynthesis pathway (sixth step). Catalyzes the cyclization reaction that produces D-erythro-imidazole glycerol phosphate. Probable cyclase HisF Rv1605, (MTV046.03), len: 267 aa. Probable hisF, cyclase involved in histidine biosynthetic pathway, similar to many e.g. AF0304|AF030405_1 Corynebacterium glutamicum cyclase (257 aa), FASTA scores: opt: 1201, E(): 0, (71.9% identity in 256 aa overlap). Belongs to the HisA/HisF family. P9WMM3 No intermediary metabolism and respiration O53908 P9WMM3 GO:0000105,GO:0000107,GO:0016829,GO:0005737 4.1.3.- P9WMM3 NC_000962.3 Mycobrowser_v5 CDS 1812359 1813171 . + 0 Rv1613 trpA Tryptophan biosynthesis pathway (fifth - last step). The alpha subunit is responsible for the ALDOL cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate. [catalytic activity: L-serine + 1-(indol-3-YL)glycerol 3-phosphate = L-tryptophan + glyceraldehyde 3-phosphate + H(2)O.] Probable tryptophan synthase, alpha subunit TrpA Rv1613, (MTCY01B2.05), len: 270 aa. Probable trpA, tryptophan synthase alpha chain. FASTA best: O68906|TRPA_MYCIT tryptophan synthase alpha chain from Mycobacterium intracellulare (271 aa), opt: 1442, E(): 0, (85.3% identity in 265 aa overlap). P9WFY1 No intermediary metabolism and respiration P66980 P9WFY1 GO:0004834,GO:0000162 4.2.1.20 P9WFY1 NC_000962.3 Mycobrowser_v5 CDS 2393411 2393641 . + 0 Rv2132 Rv2132 Unknown Conserved hypothetical protein Rv2132, (MTCY270.36c), len: 76 aa. Conserved hypothetical protein. Function unknown but belongs to Mycobacterium tuberculosis protein family including Rv2871, Rv1241, Rv3321c, Rv1113, Rv0657c, Rv1560, Rv2104c, etc. Similarity to Mycobacterium tuberculosis protein Rv2871 (AL021924|MTV020_4, 84 aa). FASTA score: opt: 142, E(): 0.00036; 41.8% identity in 55 aa overlap O06243 No conserved hypotheticals O06243 O06243 GO:0006355,GO:0003677 NC_000962.3 Mycobrowser_v5 CDS 1807298 1807762 . - 0 Rv1608c bcpB Peroxide detoxification Probable peroxidoxin BcpB Rv1608c, (MTV046.06), len: 154 aa. Probable bcpB, peroxidoxin or bacterioferritin comigratory protein, similar to many, e.g. AE0003|ECAE000335_4 bacterioferritin comigratory protein from Escherichia coli K-12 MG1655 (156 aa), FASTA scores: opt: 329, E(): 1.2e-16, (38.2% identity in 152 aa overlap); Z97179|MLCL383_22 Mycobacterium leprae cosmid L383 (161 aa) (40.2% identity in 132 aa overlap). Also similar to Rv2428 AhpC, alkyl hydroperoxide reductase from Mycobacterium tuberculosis; and other Mycobacterium tuberculosis putative peroxidoxins Rv2521, Rv2238c, Rv1932. P9WID9 No virulence, detoxification, adaptation O53911 P9WID9 GO:0045454,GO:0016491,GO:0016209 P9WID9 NC_000962.3 Mycobrowser_v5 CDS 1809443 1810150 . + 0 Rv1610 Rv1610 Unknown Possible conserved membrane protein Rv1610, (MTCY01B2.02), len: 235 aa. Possible conserved membrane protein. Equivalent to AL049913|MLCB1610_23 hypothetical protein from Mycobacterium leprae (264 aa), FASTA score: (65.8% identity in 231 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06128 No cell wall and cell processes O06128 O06128 NC_000962.3 Mycobrowser_v5 CDS 1852928 1853122 . + 0 Rv1642 rpmI Translation 50S ribosomal protein L35 RpmI Rv1642, (MTCY06H11.06), len: 64 aa. rpmI, 50S ribosomal protein L35, similar to several e.g. RL35_SYNY3|P48959 from Synechocystis sp. (67 aa), fasta scores: opt: 179, E(): 2.7e-08, (51.6% identity in 64 aa overlap). Belongs to the L35P family of ribosomal proteins. P9WH91 No information pathways P66271 P9WH91 GO:0003735,GO:0006412,GO:0005840 P9WH91 NC_000962.3 Mycobrowser_v5 CDS 1810240 1811058 . + 0 Rv1611 trpC Tryptophan biosynthesis pathway (fourth step) [catalytic activity: 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-(indol-3-YL)glycerol 3-phosphate + CO(2) + H(2)O.] Probable indole-3-glycerol phosphate synthase TrpC Rv1611, (MTCY01B2.03), len: 272 aa. Probable trpC, indole-3-glycerol phosphate synthase. Similar to Q55508|SLR0546 hypothetical 33.0 kDa protein from synechocystis SP (295 aa), FASTA score: opt: 26, E(): 7.6e-32, (44.2% identity in 265 aa overlap); also similar to TRPC_AZOBR|P26938 ndole-3-glycerol-phosphate synthaseindole-3-glycerol-phosphate synthase from Azospirillum brasilense (262 aa), FASTA score: opt: 596, E(): 4.8e-30, (43.8% identity in 258 aa overlap). Equivalent to AL0499 13|MLCB1610_24 from Mycobacterium leprae (272 aa) (90.8% identity in 272 aa overlap). Contains indole-3-glycerol phosphate synthase signature (PS00614). Belongs to the TrpC family. P9WFX7 No intermediary metabolism and respiration 3QJA P0A632 P9WFX7 GO:0000162,GO:0004425 4.1.1.48 P9WFX7 NC_000962.3 Mycobrowser_v5 CDS 1830070 1830561 . - 0 Rv1628c Rv1628c Function unknown Conserved protein Rv1628c, (MTCY01B2.20c), len: 163 aa. Conserved protein, some similarity to others e.g. Q51796 ACAC protein in Pyrococcus furiosus (136 aa), FASTA scores: opt: 199, E(): 4.6e-06, (34.7% identity in 121 aa overlap). O06145 No conserved hypotheticals O06145 O06145 O06145 NC_000962.3 Mycobrowser_v5 CDS 1813171 1814577 . + 0 Rv1614 lgt Prolipoprotein modification Possible prolipoprotein diacylglyceryl transferases Lgt Rv1614, (MTCY01B2.06), len: 468 aa. Possible lgt, prolipoprotein diacylglyceryl transferases, similar to many prolipoprotein diacylglyceryl transferases. FASTA scores: LGT_STAAU|P52282 prolipoprotein diacylglyceryl transferase from Staphylococcus aureus subsp. (279 aa), opt: 289, E():3.6e- 09, (31.5% identity in 257 aa overlap); AL096884|SC4G6_3 cosmid 4G6 from Streptomyces coelicolor (343 aa), opt: 735, E(): 4e-32, (46.5% identity in 391 aa overlap). P9WK93 No cell wall and cell processes O06131 P9WK93 GO:0042158,GO:0008961,GO:0005886,GO:0009249,GO:0016021 2.4.99.- P9WK93 NC_000962.3 Mycobrowser_v5 CDS 1815683 1816081 . + 0 Rv1616 Rv1616 Function unknown Conserved membrane protein Rv1616, (MTCY01B2.08), len: 132 aa. Conserved membrane protein, with some similarity to other hypothetical proteins e.g. AL096884|SC4G6_9 from Streptomyces coelicolor cosmid 4G6 (148 aa), FASTA scores: opt: 245, E(): 1.7e-1 0, (36.7% identity in 128 aa overlap); Q55401|SLL0543 hypothetical 16.5 kDa protein from synechocystis SP (148 aa), FASTA scores: opt: 225, E(): 6.5e-10, (35.9% identity in 117 aa overlap). Has cysteine cluster and contains a rubredoxin signature (PS00202). O06133 No cell wall and cell processes O06133 O06133 NC_000962.3 Mycobrowser_v5 CDS 1816189 1817607 . + 0 Rv1617 pykA Produces phosphoenol pyruvate in glycolysis [catalytic activity: ATP + pyruvate = ADP + phosphoenolpyruvate] Probable pyruvate kinase PykA Rv1617, (MTCY01B2.09), len: 472 aa. Probable pykA, pyruvate kinase. FASTA best: Q46078 pyruvate kinase from corynebacterium glutamicum (475 aa), opt: 2221, E(): 0, (72.2% identity in 468 aa overlap). Belongs to the pyruvate kinase family. Phosphorylated in vitro by PknJ|Rv2088 (See Arora et al., 2010). P9WKE5 No intermediary metabolism and respiration O06134 P9WKE5 GO:0006096,GO:0000287,GO:0030955,GO:0004743,GO:0005524 2.7.1.40 P9WKE5 NC_000962.3 Mycobrowser_v5 CDS 1817615 1818517 . + 0 Rv1618 tesB1 Involved in fatty acid metabolism. Probable acyl-CoA thioesterase II TesB1 Rv1618, (MTCY01B2.10), len: 300 aa. Probable tesB1, acyl-CoA thioesterase II, similar to other acyl-CoA thioesterases e.g. TESB_ECOLI|P23911 acyl-CoA thioesterase II from Escherichia coli (285 aa), FASTA scores: opt: 495, E(): 2.9e-27, (32.5% identity in 283 aa overlap); etc. Also similar to Rv2605c|tesB2 from M. tuberculosis. O06135 No lipid metabolism O06135 O06135 GO:0016291,GO:0006637 3.1.2.- O06135 NC_000962.3 Mycobrowser_v5 CDS 1818575 1820029 . + 0 Rv1619 Rv1619 Function unknown Conserved membrane protein Rv1619, (MTCY01B2.11), len: 484 aa. Conserved membrane protein. Some similarity to N-terminus of P94974|Rv1640c|MTCY06H11.04c probable lysyl-tRNA synthetase 2 from Mycobacterium tuberculosis (1172 aa), FASTA scores: E(): 1.4e-16, (28.0% identity in 410 aa overlap); and similar in part to O69916| SC3C8.03C Putative intergral membrane protein from Streptomyces coelicolor cosmid 3C8 (589 aa), FASTA scores: opt: 453 E(): 8.4e-22, (31.3% identity in 313 aa overlap). O06136 No cell wall and cell processes O06136 O06136 NC_000962.3 Mycobrowser_v5 CDS 1828865 1830073 . - 0 Rv1627c Rv1627c Thought to be involved in lipid metabolism. Probable nonspecific lipid-transfer protein Rv1627c, (MTCY01B2.19c), len: 402 aa. Probable nonspecific lipid-transfer protein, similar to many lipid carrier proteins e.g. Q51797 acetyl CoA synthase from Pyrococcus furiosus (388 aa), FASTA scores: opt: 400, E(): 3.2e-18, (34.4% identity in 407 aa overlap); etc. Also some similarity to Mycobacterium tuberculosis proteins Rv3523, Rv3540c, Rv0244, Rv2790c, Rv1323, etc. O06144 No lipid metabolism O06144 GO:0016747,GO:0008152 O06144 NC_000962.3 Mycobrowser_v5 CDS 1821690 1823273 . - 0 Rv1621c cydD Involved in active transport across the membrane of component linked with the assembly of cytochrome: involved in cytochrome biogenesis (aerobic respiration). Responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane. Probable 'component linked with the assembly of cytochrome' transport transmembrane ATP-binding protein ABC transporter CydD Rv1621c, (MTCY01B2.13c), len: 527 aa. Probable cydD, transmembrane ATP-binding protein ABC transporter involved in transport of component linked with the assembly of cytochrome (see citation below), similar to others e.g. P94366|CYDC_BACSU transport ATP-binding protein from Bacillus subtilis (567 aa), FASTA scores: opt: 784, E(): 0, (30.1% identity in 535 aa overlap); N-terminal part of AL034355|SCD78_14 from Streptomyces coelicolor (1172 aa), FASTA scores: opt: 1295, E(): 0, (44.6% identity in 534 aa overlap); etc. Also similar to Q11019|Y07D_MYCTU from Mycobacterium tuberculosis (579 aa), FASTA scores: opt: 530, E(): 6.9e-25, (29.1% identity in 530 aa overlap). Contains (PS00211) ABC transporters family signature, and (PS00017) ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters). O06138 No intermediary metabolism and respiration O06138 O06138 GO:0042626,GO:0016021,GO:0055085,GO:0005524 O06138 NC_000962.3 Mycobrowser_v5 CDS 1823360 1824400 . - 0 Rv1622c cydB Involved in the respiratory chain (at the terminal step): aerobic respiration. Cytochrome D terminal oxidase complex is the component of the aerobic respiratory chain that is supposedly predominant when cells are grown at low aeration [catalytic activity: ubiquinol-8 + O(2) = ubiquinone-8 + H(2)O]. Probable integral membrane cytochrome D ubiquinol oxidase (subunit II) CydB (cytochrome BD-I oxidase subunit II) Rv1622c, (MTCY01B2.14c), len: 346 aa. Probable cydB, cytochrome D ubiquinol oxidase subunit II, integral membrane protein, similar to others e.g. P11027|CYDB_ECOLI cytochrome D ubiquinol oxidase subunit II from Escherichia coli strain K12 (379 aa), FASTA scores: opt: 519, E(): 0, (32.3% identity in 372 aa overlap); P94365|CYDB_BACSU cytochrome D ubiquinol oxidase subunit II from Bacillus subtilis (338 aa), FASTA scores: opt: 824, E(): 0, (39.5% identity in 337 aa overlap); etc. O06139 No intermediary metabolism and respiration O06139 O06139 GO:0055114,GO:0016491,GO:0016020 1.10.3.- NC_000962.3 Mycobrowser_v5 CDS 1835013 1836236 . + 0 Rv1631 coaE Catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme a to form coenzyme a [catalytic activity: ATP + dephospho-CoA <=> ADP + CoA]. Probable dephospho-CoA kinase CoaE (dephosphocoenzyme a kinase) Rv1631, (MTCY01B2.23), len: 407 aa. Probable coaE, dephospho-CoA kinase, similar to many e.g. Q50178|ML1383|COAE_MYCLE dephospho-CoA kinase from Mycobacterium leprae (410 aa), FASTA scores: E(): 0, (77.5% identity in 409 aa overlap). Has ATP/GTP-binding site motif A (P-loop, PS00017) at N-terminus. In the N-terminal section; belongs to the CoaE family. In the C-terminal section; belongs to the UPF0157 (GrpB) family. P9WPA3 No intermediary metabolism and respiration P63826 P9WPA3 GO:0015937,GO:0005737,GO:0004140,GO:0005524 2.7.1.24 P9WPA3 NC_000962.3 Mycobrowser_v5 CDS 1843741 1846659 . + 0 Rv1638 uvrA Involved in nucleotide excision repair. The ABC excision nuclease is a DNA repair enzyme that catalyzes the excision reaction of UV-damaged nucleotide segments producing oligomers having the modified base(S). UVRA is an ATPase and a DNA-binding protein that preferentially binds single-stranded or UV-irradiated double-stranded DNA. Probable excinuclease ABC (subunit A - DNA-binding ATPase) UvrA Rv1638, (MTCY06H11.01,MTCY06H11.02c), len: 972 aa. Probable uvrA, excinuclease ABC, subunit A; DNA-binding ATPase (see citations below), similar to many e.g. UVRA_ECOLI|P07671 excinuclease abc subunit A from Escherichia coli (940 aa), FASTA scores: opt: 2573, E(): 0, (56.2% identity in 951 aa overlap). Contains 2x PS00017 ATP/GTP-binding site motif A, PS00211 ABC transporters family signature, PS00211 ABC transporters family signature. Consists of three subunits; UVRA, UVRB and UVRC. Belongs to the ABC transporter family. UVRA subfamily. P9WQK7 No information pathways 3ZQJ P63380 P9WQK7 GO:0016887,GO:0003677,GO:0009432,GO:0005737,GO:0009381,GO:0009380,GO:0046872,GO:0006289,GO:0005524 P9WQK7 NC_000962.3 Mycobrowser_v5 CDS 1848517 1852035 . - 0 Rv1640c lysX Charging LYS tRNA [catalytic activity: ATP + L-lysine + tRNA(LYS) = AMP + diphosphate + L-lysyl-tRNA (LYS)] Lysyl-tRNA synthetase 2 LysX Rv1640c, (MTCY06H11.04c), len: 1172 aa. lysX, lysyl-tRNA synthetase 2, probable two domain protein. N-terminal part (bases 1850153 to 1852033) is similar to AL023861|SC3C8_3 hypothetical membrane protein from Streptomyces coelicolor (589 aa), Fasta scores: opt: 1426, E(): 0, (44.6% identity in 585 aa overlap). The C-terminal part is similar to SYK_CRILO|P37879 lysyl-tRNA synthetases from Cricetulus longicaudatus (Long-tailed hamster) (597 aa), Fasta scores, opt: 985, E(): 0, (36.8% identity in 524 aa overlap). Contains PS00179 Aminoacyl-transfer RNA synthetases class-II signature 1, PS00339 Aminoacyl-transfer RNA synthetases class-II signature 2. This may indicate a frame shift but sequence has been checked and no error found. Belongs to class-II aminoacyl-tRNA synthetase family. P9WFU7 No information pathways P94974 P9WFU7 GO:0005737,GO:0016021,GO:0006629,GO:0004824,GO:0006430,GO:0050071,GO:0000287,GO:0003676,GO:0009405,GO:0005886,GO:0046677,GO:0005524 2.3.2.3,6.1.1.6 P9WFU7 NC_000962.3 Mycobrowser_v5 CDS 1825998 1826585 . - 0 Rv1624c Rv1624c Unknown Probable conserved membrane protein Rv1624c, (MTCY01B2.16c), len: 195 aa. Probable membrane protein, first start taken. Some similarity to Rv3155 nuoK, NADH dehydrogenase chain K from M. tuberculosis. Also similar to AAK72093.1|AF196488 hypothetical protein from Mycobacterium smegmatis (205 aa). Identities = 117/195 (60%). O06141 No cell wall and cell processes O06141 O06141 NC_000962.3 Mycobrowser_v5 CDS 1828180 1828797 . + 0 Rv1626 Rv1626 Sensor part of a two component regulatory system Probable two-component system transcriptional regulator Rv1626, (MTCY01B2.18), len: 205 aa. Probable two-component response system transcriptional regulator, similar to many e.g. CHEY_BACSU|P24072 chemotaxis protein chey homolog (119 aa), FASTA scores: opt: 283, E(): 1.6e-16, (43.0% identity in 114 aa overlap). Also similar to AL109732|SC7H2_27 hypothetical protein from Streptomyces coelicolor (218 aa), opt: 880, E(): 0, (69.4% identity in 196 aa overlap). P9WGM3 No regulatory proteins 1S8N,1SD5 O06143 P9WGM3 GO:0031564,GO:0000156,GO:0000160,GO:0006350 P9WGM3 NC_000962.3 Mycobrowser_v5 CDS 1846989 1848458 . - 0 Rv1639c Rv1639c Function unknown Conserved hypothetical membrane protein Rv1639c, (MTCY06H11.03c), len: 489 aa. Conserved hypothetical membrane protein. Some similarity to P35866|YLI2_CORGL Hypothetical 45.7 kDa protein from Corynebacterium glutamicum (426 aa), FASTA scores: opt: 511, E(): 2.4e-23, (28.9% identity in 370 aa overlap). Contains PS00904 protein phenyltransferases alpha subunit repeat signature P94973 No cell wall and cell processes P94973 P94973 P94973 NC_000962.3 Mycobrowser_v5 CDS 1854399 1855454 . - 0 Rv1645c Rv1645c Function unknown Conserved hypothetical protein Rv1645c, (MTCY06H11.10c), len: 351 aa. Conserved hypothetical protein, similar to other Mycobacterium tuberculosis hypothetical proteins e.g. O53837|Rv0826|MTV043.18 (351 aa), FASTA scores: (57.5% identity in 299 aa overlap); Q10519|Rv2237|YM37_MYCTU (255 aa), O53682|Rv0276 (306 aa). P94979 No conserved hypotheticals P94979 P94979 P94979 NC_000962.3 Mycobrowser_v5 CDS 2201223 2201744 . - 0 Rv1954c Rv1954c Unknown Hypothetical protein Rv1954c, (MTCY09F9.10), len: 173 aa. Hypothetical unknown protein, end overlaps next ORF upstream, Rv1955 (MTCY09F9.09c). P9WLQ3 No conserved hypotheticals P9WLQ3 NC_000962.3 Mycobrowser_v5 CDS 1839168 1840583 . + 0 Rv1634 Rv1634 Thought to be involved in transport of drug across the membrane (export). Drug resistance by an export mechanism (confers resistance to toxic compounds by removing them for the cells). Possible drug efflux membrane protein Rv1634, (MTCY01B2.26), len: 471 aa. Possible drug efflux membrane protein of major facilitator superfamily (MFS), similar to many antibiotic resistance (efflux) proteins. FASTA best: Q56175 TU22 dTDP-glucose dehydrtatase (GRAE) from Streptomyces violaceoruber (557 aa), opt: 415, E(): 1.7e-17, (26.7% identity in 446 aa overlap). Relatives in Mycobacterium tuberculosis: MTCY369.27c, E(): 4.8e-12; MTCY20B11.14c, E(): 2.9e-10. P9WJX3 No cell wall and cell processes O06151 P9WJX3 GO:0005886,GO:0046677,GO:0055085,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1840572 1842242 . - 0 Rv1635c Rv1635c Possibly involved in the biosynthesis of lipoarabinomannan (lam) Probable mannosyltransferase. Probable conserved transmembrane protein. Rv1635c, (MTCY01B2.27c), len: 556 aa. Probable mannosyltransferase (See Dinadayala et al., 2006). Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). Probable conserved transmembrane protein, equivalent to CAC31770.1|AL583921 Mycobacterium leprae membrane protein (527 aa), Identities = 332/527 (62%). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06152 No cell wall and cell processes O06152 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1842451 1842891 . + 0 Rv1636 TB15.3 Function unknown Iron-regulated universal stress protein family protein TB15.3 Rv1636, (MTCY01B2.28), len: 146 aa. TB15.3, iron-regulated universal stress protein family protein (see citations below), similar to other hypothetical proteins from diverse organisms e.g. Q57951|MJ0531|Y531_METJA from Methanococcus jannaschii (170 aa), FASTA scores: opt: 188, E(): 6e-06, (32.2% identity in 149 aa overlap); also P42297|YXIE_BACSU hypothetical 15.9 kDa protein in bglh-wapa intergenic region precursor from Bacillus subtilis (148 aa), FASTA scores: opt: 162, E(): 0.00025, (30.8% identity in 156 aa overlap). Part of family of Mycobacterium tuberculosis hypothetical proteins (but lacks C-terminal region) including Rv2005c, Rv2623, Rv2026c, Rv1996, etc. P9WFC9 No virulence, detoxification, adaptation 1TQ8 O06153 P9WFC9 GO:0006950 P9WFC9 NC_000962.3 Mycobrowser_v5 CDS 1842898 1843692 . - 0 Rv1637c Rv1637c Function unknown Conserved protein Rv1637c, (MTCY01B2.29c,MTCY06H11.01c), len: 264 aa. Conserved protein, some similarity to others e.g. P05446|GLO2_RHOBL probable hydroxyacylglutathione hydrolase (255 aa), FASTA scores: opt: 252, E(): 2e-09, (39.0% identity in 146 aa overlap). Also similar to Q9Z505|AL035591|SCC54.20 putative hydrolase from Streptomyces coelicolor (218 aa), FASTA scores: opt: 732, E(): 0, (52.3% identity in 220 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins and putative glyoxylases e.g. Rv0634c, Rv3677c, Rv2581c, Rv2260. O06154 No conserved hypotheticals O06154 GO:0016787 O06154 NC_000962.3 Mycobrowser_v5 CDS 1855764 1856696 . + 0 Rv1646 PE17 Function unknown PE family protein PE17 Rv1646, (MTCY06H11.11), len: 310 aa. PE17, Member of the Mycobacterium tuberculosis PE family of proteins (see citation below), similar to many e.g. YW36_MYCTU|Q10873 hypothetical 53.7 kd protein cy39.36c (558 aa), FASTA scores, opt: 411, E(): 1.3e-15, (34.4% identity in 320 aa overlap). L7N681 No PE/PPE Q7D879 L7N681 L7N681 NC_000962.3 Mycobrowser_v5 CDS 1853184 1853573 . + 0 Rv1643 rplT This protein binds directly to 23S ribosomal RNA and is necessary to the in vitro assembly process of the 50S ribosomal subunit; it is not involved in the protein synthesizing functions of that subunit 50S ribosomal protein L20 RplT Rv1643, (MTCY06H11.07), len: 129 aa. rplT, 50S ribosomal protein L20, similar to several e.g. RL20_ECOLI|P02421 from Escherichia coli (117 aa), FASTA scores: opt: 438, E(): 5.8e-24, (60.3% identity in 116 aa overlap). Contains PS00937 Ribosomal protein L20 signature. P9WHC5 No information pathways P66105 P9WHC5 GO:0000027,GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WHC5 NC_000962.3 Mycobrowser_v5 CDS 1853606 1854388 . + 0 Rv1644 tsnR rRNA modification Possible 23S rRNA methyltransferase TsnR Rv1644, (MTCY06H11.08), len: 260 aa. Possible tsnR, 23S rRNA methyltransferase, similar to several e.g. TSNR_STRLU|P52393 from Streptomyces laurentii (270 aa), FASTA scores: opt: 276, E(): 3.6e-11, (27.6% identity in 261 aa overlap). Also similar to M. tuberculosis hypothetical proteins Rv0881, Rv3579c, and Rv0380c. P94978 No information pathways P94978 P94978 GO:0008173,GO:0006396,GO:0003723 2.1.1.- P94978 NC_000962.3 Mycobrowser_v5 CDS 1856774 1857724 . + 0 Rv1647 Rv1647 Thought to play an essential role in regulation of cellular metabolism by catalysing the synthesis of a second messenger, camp [catalytic activity: ATP = 3',5'-cyclic AMP + pyrophosphate]. Adenylate cyclase (ATP pyrophosphate-lyase) (adenylyl cyclase) Rv1647, (MTCY06H11.12), len: 316 aa. Adenylate cyclase, some similarity to other Mycobacterium tuberculosis proteins e.g. Q11055|Rv1264|YC64_MYCTU 42.2 kDa protein (397 aa), FASTA scores: opt: 197, E(): 9.4e-06, (27.1% identity in 181 aa overlap) and Q10400|Rv2212|YM12_MYCTU (378 aa). Belongs to adenylyl cyclase class-3 family. P94982 No intermediary metabolism and respiration P94982 P94982 GO:0016849,GO:0009190 P94982 NC_000962.3 Mycobrowser_v5 CDS 1858733 1859758 . + 0 Rv1649 pheS Charging PHE tRNA [catalytic activity: ATP + L-phenylalanine + tRNA(PHE) = AMP + diphosphate + L-phenylalanyl-tRNA(PHE)] Probable phenylalanyl-tRNA synthetase, alpha chain PheS Rv1649, (MTCY06H11.14), len: 341 aa. Probable pheS, Phenylalanyl-tRNA synthetase alpha chain, similar to several e.g. SYFA_ECOLI|P08312 from Escherichia coli (327 aa), FASTA scores: opt: 978, E(): 0, (46.5% identity in 331 aa overlap). Homology suggests this start site, but there is a potential rbs upstream of a gtg 30 bp upstream; contains PS00179 Aminoacyl-transfer RNA synthetases class-II signature 1. Belongs to class-II aminoacyl-tRNA synthetase family. PHE-tRNA synthetase alpha chain subfamily 1. P9WFU3 No information pathways P94984 P9WFU3 GO:0005737,GO:0000287,GO:0004826,GO:0006432,GO:0000049,GO:0005524 6.1.1.20 P9WFU3 NC_000962.3 Mycobrowser_v5 CDS 1869922 1870845 . + 0 Rv1656 argF Involved in arginine biosynthesis [catalytic activity: carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.] Probable ornithine carbamoyltransferase, anabolic ArgF Rv1656, (MTCY06H11.21), len: 307 aa. Probable argF, ornithine carbamoyltransferase, anabolic (see citation below), almost identical to OTCA_MYCBO|Q02095 ornithine carbamoyltransferase, anabolic from Mycobacterium bovis (307 aa), FASTA scores: opt: 1980, E(): 0, (99.0% identity in 307 aa overlap); contains PS00097 Aspartate and ornithine carbamoyltransferases signature. Belongs to the ATCases/OTCases family. P9WIT9 No intermediary metabolism and respiration 2I6U,2P2G P0A5M8 P9WIT9 GO:0006526,GO:0004585,GO:0009348,GO:0016597 2.1.3.3 P9WIT9 NC_000962.3 Mycobrowser_v5 CDS 1967705 1967917 . + 0 Rv1740 vapB34 Unknown Possible antitoxin VapB34 Rv1740, (MTCY28.02-MTCY04C12.25), len: 70 aa. Possible vapB34, antitoxin, part of toxin-antitoxin (TA) operon with Rv1741, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. P96913|Rv0623|MTCY20H10.04 (84 aa), (73.5% identity in 68 aa overlap); P71998|Rv1740 (70 aa), and O07770|Rv0608 (81 aa). P9WJ31 No virulence, detoxification, adaptation P71998 P9WJ31 P9WJ31 NC_000962.3 Mycobrowser_v5 CDS 1865576 1866634 . + 0 Rv1652 argC Involved in arginine biosynthesis (at the third step) [catalytic activity: N-acetyl-L-glutamate 5-semialdehyde + NADP(+) + phosphate = N-acetyl-5-glutamyl phosphate + NADPH]. Probable N-acetyl-gamma-glutamyl-phoshate reductase ArgC Rv1652, (MTCY06H11.17), len: 352 aa. Probable argC, N-acetyl-gamma-glutamyl-phosphate reductase, similar to many e.g. ARGC_STRCL|P54896 from Streptomyces clavuligerus (340 aa), FASTA scores: opt: 1119, E(): 0, (56.9% identity in 350 aa overlap); etc. Belongs to the NAGSA dehydrogenase family. P9WPZ9 No intermediary metabolism and respiration 2I3A,2I3G,2NQT P63562 P9WPZ9 GO:0051287,GO:0006526,GO:0005737,GO:0055114,GO:0046983,GO:0003942 1.2.1.38 P9WPZ9 NC_000962.3 Mycobrowser_v5 CDS 1866631 1867845 . + 0 Rv1653 argJ Arginine biosynthesis. Probable glutamate N-acetyltransferase ArgJ Rv1653, (MTCY06H11.18), len: 404 aa. Probable argJ, Glutamate n-acetyltransferase, similar to ARGJ_BACSU|P36843 from Bacillus subtilis (406 aa), fasta scores: opt: 727, E(): 0, (36.3% identity in 410 a a overlap). P9WPZ3 No intermediary metabolism and respiration 3IT4,3IT6 P63571 P9WPZ3 GO:0006526,GO:0005737,GO:0004358,GO:0004042 2.3.1.1,2.3.1.35 P9WPZ3 NC_000962.3 Mycobrowser_v5 CDS 1999142 1999357 . - 0 Rv1765A Rv1765A Possibly required for the transposition of an insertion element. Putative transposase (fragment) Rv1765A, len: 71 aa. Putative transposase (fragment), similar to part of many transposase genes including IS6110 e.g. P19774|TRA9_MYCTU putative transposase from Mycobacterium tuberculosis (278 aa), FASTA scores: opt: 231, E(): 4.7e-11, (45.35% identity in 75 aa overlap). Q79FL0 No insertion seqs and phages Q79FL0 Q79FL0 GO:0015074,GO:0003677 NC_000962.3 Mycobrowser_v5 CDS 1867842 1868726 . + 0 Rv1654 argB Arginine biosynthesis (second step) [catalytic activity: ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate] Probable acetylglutamate kinase ArgB Rv1654, (MTCY06H11.19), len: 294 aa. Probable argB, Acetylglutamate kinase, similar to ARGB_CORGL|Q59281 (294 aa), FASTA scores: opt: 1209, E(): 0, (64.4% identity in 270 aa overlap). Belongs to the acetylglutamate kinase family. P9WQ01 No intermediary metabolism and respiration 2AP9 P0A4Y6 P9WQ01 GO:0003991,GO:0006526,GO:0005737,GO:0004349,GO:0006561,GO:0005524 2.7.2.8 P9WQ01 NC_000962.3 Mycobrowser_v5 CDS 1868723 1869925 . + 0 Rv1655 argD Arginine biosynthesis (fourth step) [catalytic activity: N2-acetyl-L-ornithine + 2-oxoglutarate = N-acetyl-L-glutamate 5-semialdehyde + L-glutamate] Probable acetylornithine aminotransferase ArgD Rv1655, (MTCY06H11.20), len: 400 aa. Probable argD, Acetylornithine aminotransferase, similar to ARGD_ECOLI|P18335 (406 aa), FASTA scores: opt: 958, E(): 0, (38.6% identity in 404 aa overlap), contains PS00600 Aminotransferases class-III pyridoxal-phosphate attachment site. Belongs to class-III of pyridoxal-phosphate-dependent aminotransferases. P9WPZ7 No intermediary metabolism and respiration P63568 P9WPZ7 GO:0006526,GO:0005737,GO:0030170,GO:0003992 2.6.1.11 P9WPZ7 NC_000962.3 Mycobrowser_v5 CDS 1910776 1911399 . - 0 Rv1685c Rv1685c Function unknown Conserved hypothetical protein Rv1685c, (MTCI125.07c), len: 207 aa. Conserved hypothetical protein, some similarity to other Mycobacterium tuberculosis hypothetical regulatory proteins e.g. Q10774|Rv1556|YF56_MYCTU (202 aa), FASTA scores: opt: 111, E(): 1.7e-05, (24.1% identity in 195 aa overlap); and P95215|Rv0258c|MTCY06A4.02c (151 aa) FASTA scores: (32.9% identity in 140 aa overlap); also similar to Q9X8G9|SCE7.13C|AL049819 putative Streptomyces coelicolor transcriptional regulator (204 aa), FASTA scores: opt: 480, E(): 6.4e-25, (40.4% identity in 203 aa overlap). O33187 No conserved hypotheticals O33187 O33187 GO:0006350,GO:0003700,GO:0006355 O33187 NC_000962.3 Mycobrowser_v5 ncRNA 1960667 1960783 . + 0 MTB000077 MTS1338 Unknown Putative small regulatory RNA MTS1338, putative small regulatory RNA (See Arnvig et al., 2011), 5'-end mapped by RLM-RACE, alternate 5'-end at position 1960601, ~100 bp band detected by Northern blot. No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 1871363 1872559 . + 0 Rv1658 argG Arginine biosynthesis [catalytic activity: ATP + L-citrulline + L-aspartate = AMP + diphosphate + L-argininosuccinate] Probable argininosuccinate synthase ArgG Rv1658, (MTCY06H11.23), len: 398 aa. Probable argG, Argininosuccinate synthase, similar to ASSY_STRCL|P50986 argininosuccinate synthase from Streptomyces clavuligerus (397 aa), FASTA scores: opt: 1873, E(): 0, (67.8% identity in 397 aa overlap); contains PS00564 Argininosuccinate synthase signature 1, PS00565 Argininosuccinate synthase signature 2. Belongs to the argininosuccinate synthase family. P9WPW7 No intermediary metabolism and respiration P63642 P9WPW7 GO:0006526,GO:0004055,GO:0005737,GO:0005524 6.3.4.5 P9WPW7 NC_000962.3 Mycobrowser_v5 CDS 1872639 1874051 . + 0 Rv1659 argH Arginine biosynthesis (last step) [catalytic activity: N-(L-arginino)succinate = fumarate + L- arginine] Probable argininosuccinate lyase ArgH Rv1659, (MTCY06H11.24), len: 470 aa. Probable argH, Argininosuccinate lyase, similar to ARLY_ECOLI|P11447 argininosuccinate lyase from Escherichia coli (457 aa), FASTA scores: opt: 1091, E(): 0, (42.5% identity in 461 aa overlap); contains PS00017 ATP/GTP-binding site motif A, PS00163 Fumarate lyases signature. Belongs to the lyase 1 family. Argininosuccinate lyase subfamily. P9WPY7 No intermediary metabolism and respiration P0A4Z0 P9WPY7 GO:0004056,GO:0005737,GO:0042450 4.3.2.1 P9WPY7 NC_000962.3 Mycobrowser_v5 CDS 1874160 1875221 . + 0 Rv1660 pks10 Possibly involved in the biosynthesis of secondary metabolites [catalytic activity: 3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2] Chalcone synthase Pks10 Rv1660, (MTCY06H11.25), len: 353 aa. pks10, chalcone synthase, similar to BCSA_BACSU|P54157 putative chalcone synthase from B. subtilis (365 aa), FASTA scores: opt: 701, E(): 0, (33.1% identity in 362 aa overlap). Also similar to M. tuberculosis Rv1665|pks11 polyketide synthase (chalcone synthase); and Rv1372|pks18 polyketide synthase. Other upstream initiation sites are possible but homology suggests this start. Note pks10 has been shown to be involved in the biosynthesis of phthiocerol. P9WPF5 No lipid metabolism P94995 P9WPF5 GO:0009058,GO:0016210,GO:0008415 2.3.1.74 P9WPF5 NC_000962.3 Mycobrowser_v5 CDS 1875304 1881684 . + 0 Rv1661 pks7 Potentially involved in some intermediate steps for the synthesis of a polyketide molecule which may be involved in secondary metabolism Probable polyketide synthase Pks7 Rv1661, (MTCY06H11.26), len: 2126 aa. Probable pks7, polyketide synthase, similar to many e.g. ERY2_SACER|Q03132 erythronolide synthase, modules 3 and 4 (3567 aa), FASTA scores: E(): 0, (48.8% identity in 2131 aa overlap); also similar to Mycobacterium tuberculosis pks12. Contains PS00606 Beta-ketoacyl synthases active site, PS00012 Phosphopantetheine attachment site. P94996 No lipid metabolism P94996 P94996 GO:0009058,GO:0048037,GO:0055114,GO:0016491,GO:0031177,GO:0016740,GO:0008270,GO:0000036 P94996 NC_000962.3 Mycobrowser_v5 CDS 2727336 2727920 . - 0 Rv2430c PPE41 Function unknown PPE family protein PPE41 Rv2430c, (MTCY428.16), len: 194 aa. PPE41, Member of the Mycobacterium tuberculosis PPE family similar to others e.g. AAK46014|Rv1745|MT1745 from Mycobacterium tuberculosis (385 aa) FASTA scores: opt: 389, E(): 1.2e-17, (35.95% identity in 192 aa overlap); etc. Q79FE1 No PE/PPE 2G38 Q79FE1 Q79FE1 Q79FE1 NC_000962.3 Mycobrowser_v5 CDS 1881704 1886512 . + 0 Rv1662 pks8 Potentially involved in some intermediate steps for the synthesis of a polyketide molecule which may be involved in secondary metabolism Probable polyketide synthase Pks8 Rv1662, (MTCY275.01-MTCY06H11.27), len: 1602 aa. Probable pks8, polyketide synthase, similar to many polyketide synthases e.g. ERY2_SACER|Q03132 erythronolide synthase, modules 3 and 4 from Saccharopolyspora erythraea (Streptomyces erythraeus) (3567 aa), FASTA scores: opt: 3319, E(): 0, (45.8% identity in 1619 aa overlap). Also similar to other Mycobacterium tuberculosis probable polyketide synthases e.g. pks7 and pks12. Contains PS00606 Beta-ketoacyl synthases active site and PS01162 Quinone oxidoreductase/zeta-crystallin signature. Note that the similarity extends into the downstream ORF Rv1663 (MTCY275.02), and this could be accounted for by a frameshift, although the sequence has been checked and no discrepancy was found. O65933 No lipid metabolism O65933 O65933 GO:0048037,GO:0055114,GO:0016491,GO:0016740,GO:0008270,GO:0009058 O65933 NC_000962.3 Mycobrowser_v5 CDS 2195344 2195934 . - 0 Rv1944c Rv1944c Function unknown Conserved protein Rv1944c, (MTCY09F9.20), len: 196 aa. Conserved protein, similar to C-terminal part of SCE20.29|AL136058|CAB65585.1 hypothetical protein from Streptomyces coelicolor (338 aa), blastp scores, Identities = 37/131 (28%), Positives = 51/131 (38%). P95270 No conserved hypotheticals P95270 P95270 NC_000962.3 Mycobrowser_v5 CDS 2200190 2200486 . - 0 Rv1951c Rv1951c Function unknown Conserved hypothetical protein Rv1951c, (MTCY09F9.13), len: 98 aa. Conserved hypothetical protein, similar to Mycobacterium tuberculosis hypothetical protein Rv2541 (135 aa) (40.9% identity in 88 aa overlap). P95263 No conserved hypotheticals P95263 NC_000962.3 Mycobrowser_v5 CDS 1893577 1894230 . - 0 Rv1667c Rv1667c Thought to be involved in active transport of macrolide across the membrane (export). Macrolide antibiotics resistance by an export mechanism. Responsible for energy coupling to the transport system. Probable second part of macrolide-transport ATP-binding protein ABC transporter Rv1667c, (MTV047.03c), len: 217 aa. Probable second part of macrolide-transport ATP-binding protein ABC transporter (see citation below), with similarity to C-terminal end of putative ABC transporters/ATP binding proteins, e.g. Z99108|BSUB0005_6 ABC transporter (ATP-binding protein) homolog yfmR from Bacillus subtilis (629 aa), FASTA scores: opt: 411, E(): 6.9e-17, (37.8% identity in 217 aa overlap); etc. Similarity to other NBD components of ABC transporters suggests that Rv1667c and Rv1668c should be contiguous. However, sequence has been checked and no errors found, also same sequence in M. tuberculosis CSU93 and Mycobacterium bovis. O53915 No cell wall and cell processes O53915 O53915 GO:0016887,GO:0005524 O53915 NC_000962.3 Mycobrowser_v5 CDS 1888026 1891079 . + 0 Rv1664 pks9 Potentially involved in some intermediate steps for the synthesis of a polyketide molecule which may be involved in secondary metabolism Probable polyketide synthase Pks9 Rv1664, (MTCY275.03), len: 1017 aa. Probable pks9, polyketide synthase, similar to OL56_STRAT|Q07017 oleandomycin polyketide synthase, modules 5 and 6 from Streptomyces antibioticus (3519 aa), FASTA scores: opt: 1767, E(): 0, (41.6% identity in 919 aa overlap). Similar to other Mycobacterium tuberculosis probable polyketide synthases e.g. pks6, pks8, etc. Contains PS00012 Phosphopantetheine attachment site. O06586 No lipid metabolism O06586 O06586 GO:0009058,GO:0048037,GO:0031177,GO:0016740,GO:0000036 O06586 NC_000962.3 Mycobrowser_v5 CDS 1927211 1928575 . - 0 Rv1702c Rv1702c Unknown Conserved hypothetical protein Rv1702c, (MTCI125.24c), len: 454 aa. Conserved hypothetical ORF in REP13E12 degenerate repeat. Similar to other hypothetical proteins inside REP13E12 elements (often in two parts) e.g. Rv0094c|Q50655|MTCY251.13c (317 aa), FASTA scores: opt: 1284, E(): 0, (59.7% identity in 315 aa overlap); and Rv1128c, Rv1945, Rv1148c, etc. P9WLT3 No insertion seqs and phages P64885 P9WLT3 P9WLT3 NC_000962.3 Mycobrowser_v5 CDS 1895725 1896087 . + 0 Rv1669 Rv1669 Unknown Hypothetical protein Rv1669, (MTV047.04B), len: 120 aa. Hypothetical unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). O86371 No conserved hypotheticals O86371 O86371 NC_000962.3 Mycobrowser_v5 CDS 1896120 1896467 . + 0 Rv1670 Rv1670 Function unknown Conserved hypothetical protein Rv1670, (MTV047.05), len: 115 aa. Conserved hypothetical protein, highly similar to D90908|D90908_87 Hypothetical protein of Synechocystis sp. PCC6803 complete (94 aa), FASTA scores opt: 378, E(): 3.5e-2, (55.2% identity in 96 aa overlap); also shows some similarity to Mycobacterium tuberculosis hypothetical proteins e.g. C-terminal region of O53404|Rv1056 (254 aa), and P96817|Rv0140 (126 aa). O53917 No conserved hypotheticals O53917 O53917 O53917 NC_000962.3 Mycobrowser_v5 CDS 2394650 2395237 . - 0 Rv2134c Rv2134c Unknown Conserved protein Rv2134c, (MTCY270.34), len: 195 aa. Conserved protein. Function: unknown but equivalent to hypothetical Mycobacterium leprae protein, Q49789. FASTA best: Q49789 B2126_C3_228, opt: 1192, E(): 0 (91.1% identity in 192 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06241 No conserved hypotheticals O06241 O06241 NC_000962.3 Mycobrowser_v5 CDS 1898300 1899232 . - 0 Rv1673c Rv1673c Function unknown Conserved hypothetical protein Rv1673c, (MTV047.08c), len: 310 aa. Conserved hypothetical protein, shows weak similarity to P44103|YA48_HAEIN Hypothetical protein HI10 48 precursor (369 aa), FASTA scores: E(): 8.3e-11, (26.1% identity in 330 aa overlap). O53920 No conserved hypotheticals O53920 O53920 O53920 NC_000962.3 Mycobrowser_v5 CDS 1899260 1899916 . - 0 Rv1674c Rv1674c Involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv1674c, (MTV047.09c), len: 218 aa. Probable transcriptional regulatory protein. Highly similar to AJ005575|SPE005575_2 Streptomyces peucetius (226 aa), FASTA scores: opt: 662, E(): 0, (50.0% identity in 208 aa overlap). Similar to Rv0324|Z96800|MTCY63.29 M. tuberculosis cosmid (226 aa), FASTA scores: opt: 579, E(): 0, (45.3% identity in 214 aa overlap). N-terminus is similar to transcriptional activators e.g. MERR_STRLI|P30346 probable mercury resistance operon regulator (125 aa), FASTA scores: opt: 183, E(): 1.9e-06, (35.6% identity in 90 aa overlap). Contains PS00380 Rhodanese signature 1. O53921 No regulatory proteins O53921 O53921 GO:0006355,GO:0008272,GO:0004792,GO:0006350,GO:0003700,GO:0005622 O53921 NC_000962.3 Mycobrowser_v5 CDS 1901748 1902296 . + 0 Rv1677 dsbF Unknown; possibly involved in thiol:disulfide interchange. Probable conserved lipoprotein DsbF Rv1677, (MTV047.12), len: 182 aa. Probable dsbF, conserved lipoprotein possibly involved in thiol:disulfide interchange. Highly similar to C-terminus of Z74024|MTCY274.09 mpt53 soluble secreted antigen precursor from Mycobacterium tuberculosis (173 aa), FASTA scores: opt: 482, E(): 3.6e-23, (52.8% identity in 142 aa overlap) . Also some similarity to P52237|TIPB_PSEFL thiol:disulfide interchange protein TIPB precursor from Pseudomonas fluorescens (178 aa), FASTA scores: opt: 190, E(): 4.4e-05, (28.5% identity in 151 aa overlap); and P33926|DSBE_ECOLI thiol:disulfide interchange protein from Escherichia coli (185 aa), FASTA scores: opt: 194, E(): 2.6e-05, (29.1% identity in 175 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site and PS00194 Thioredoxin family active site. Nucleotide position 1901816 in the genome sequence has been corrected, A:G resulting in Q23Q. I6XYM2 No cell wall and cell processes 1ZZO,3IOS O53924 I6XYM2 GO:0016491,GO:0045454 I6XYM2 NC_000962.3 Mycobrowser_v5 CDS 1902397 1903299 . + 0 Rv1678 Rv1678 Unknown Probable integral membrane protein Rv1678, (MTV047.13), len: 300 aa. Probable integral membrane protein. O53925 No cell wall and cell processes O53925 NC_000962.3 Mycobrowser_v5 CDS 1904429 1905253 . + 0 Rv1680 Rv1680 Unknown Hypothetical protein Rv1680, (MTCI125.02), len: 274 aa. Hypothetical unknown protein. O33182 No conserved hypotheticals O33182 O33182 NC_000962.3 Mycobrowser_v5 CDS 1905250 1906242 . + 0 Rv1681 moeX Involved in molybdoptenum cofactor biosynthesis Possible molybdopterin biosynthesis protein MoeX Rv1681, (MTCI125.03), len: 330 aa. Possible moeX, Molybdopterin biosynthesis protein, has weak similarity to MOAA_ECOLI|P30745 molybdenum cofactor biosynthesis protein (329 aa), FASTA scores: opt: 162, E(): 0.00081, (27.7% identity in 224 aa overlap) and to Rv3109|MTCY164.19 MoaA from Mycobacterium tuberculosis (28.5% identity in 165 aa overlap). O33183 No intermediary metabolism and respiration O33183 O33183 GO:0051536,GO:0003824 O33183 NC_000962.3 Mycobrowser_v5 CDS 1906403 1907320 . + 0 Rv1682 Rv1682 Function unknown Probable coiled-coil structural protein Rv1682, (MTCI125.04), len: 305 aa. Probable coiled-coil structural protein, weakly similar to many paramyosins, kinesins and plectins e.g. MYSP_ONCVO|Q02171 paramyosin from onchocerca volvulus (879 aa), fasta scores: opt: 180, E():2.6e-08, (24.4% identity in 234 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical coiled-coil proteins (wag31 antigen 84) Rv2145c and Rv2927c. O33184 No conserved hypotheticals O33184 O33184 NC_000962.3 Mycobrowser_v5 CDS 1910586 1910810 . + 0 Rv1684 Rv1684 Function unknown Conserved hypothetical protein Rv1684, (MTCI125.06), len: 74 aa. Conserved hypothetical protein, similar to P75844|YCAR_ECOLI Protein YCAR from Escherichia coli (60 aa), FASTA scores: opt: 108, E(): 0.00022, (39.0% identity in 59 aa overlap). O33186 No conserved hypotheticals O33186 O33186 O33186 NC_000962.3 Mycobrowser_v5 CDS 1911401 1912081 . - 0 Rv1686c Rv1686c Thought to be involved in active transport of undetermined substrate (possibly drug) across the membrane. Responsible for the translocation of the substrate across the membrane. Probable conserved integral membrane protein ABC transporter Rv1686c, (MTCI125.08c), len: 226 aa. Probable conserved integral membrane protein ABC transporter (see citation below), similar to AL049819|SCE7.05 putative integral membrane protein from Streptomyces coelicolor (266 aa), FASTA sacores: opt: 661, E(): 0, (45.1% identity in 226 aa overlap); and Q53627|U43537 membrane protein involved in mithramycin resistance from streptomyces argillaceus (233 aa), FASTA scores: opt: 222, E(): 5.4e-10, (28.7% identity in 216 aa overlap). O33188 No cell wall and cell processes O33188 O33188 GO:0042626,GO:0016020,GO:0006810,GO:0005524 O33188 NC_000962.3 Mycobrowser_v5 CDS 2002626 2003870 . + 0 Rv1769 Rv1769 Function unknown Conserved protein Rv1769, (MTCY28.35), len: 414 aa. Conserved protein, similar to O88066|SCI35.31|AL031541 hypothetical protein from Streptomyces coelicolor (402 aa), FASTA scores: opt: 1341, E(): 0, (53.8% identity in 398 aa overlap). O06802 No conserved hypotheticals O06802 O06802 NC_000962.3 Mycobrowser_v5 ncRNA 1914962 1915190 . - 0 MTB000054 G2 Unknown Putative small regulatory RNA G2, putative small regulatory RNA (See Arnvig and Young, 2009). Alternate 5'-end at position 1915028. Alternate 3'-end at position 1914977. No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 1912979 1913590 . + 0 Rv1688 mpg Thought to be involved in base excision repair. Possible 3-methyladenine DNA glycosylase Mpg Rv1688, (MTCI125.10), len: 203 aa. Possible mpg, 3-methyladenine DNA glycosylase (see citation below), similar to several eukaryotic 3-methylpurine DNA glycosylases and 3-methyladenine DNA glycosylases e.g. Q39147|X76169 3-methyladenine glycosylase from Arabidobsis thaliana (254 aa), FASTA scores: opt: 297, E(): 8.3e-15, (31.8% identity in 198 aa overlap) and P29372|3MG_HUMAN dna-3-methyladenine glycosidase (298 aa), FASTA scores: opt: 220, E(): 7.2e-05, (36.4% identity in 184 aa overlap). Belongs to the mpg family of DNA glycosylases. P9WJP7 No information pathways P65412 P9WJP7 GO:0003905,GO:0006284,GO:0003677 3.2.2.- P9WJP7 NC_000962.3 Mycobrowser_v5 CDS 1913602 1914876 . + 0 Rv1689 tyrS Involved in translation mechanism [catalytic activity: ATP + L-tyrosine + tRNA(TYR) = AMP + diphosphate + L-tyrosyl-tRNA(TYR)] Probable tyrosyl-tRNA synthase TyrS (TYRRS) Rv1689, (MTCI125.11), len: 424 aa. Probable tyrS, Tyrosyl-tRNA synthase, highly similar to many e.g. SYY_ECOLI|P00951 Escherichia coli (423 aa), FASTA scores: opt: 1271, E(): 0, (47.3% identity in 419 aa overlap). Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Belongs to class-I aminoacyl-tRNA synthetase family. P9WFT1 No information pathways 2JAN P67611 P9WFT1 GO:0003723,GO:0005737,GO:0004831,GO:0006437,GO:0005524 6.1.1.1 P9WFT1 NC_000962.3 Mycobrowser_v5 CDS 1915527 1915910 . + 0 Rv1690 lprJ Unknown Probable lipoprotein LprJ Rv1690, (MTCI125.12), len: 127 aa. Probable lprJ, lipoprotein; contains possible signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Weakly similar to other Mycobacterium tuberculosis hypothetical proteins with conserved cysteines e.g. Rv1804c, Rv1810, Rv3354, etc O33192 No cell wall and cell processes O33192 O33192 NC_000962.3 Mycobrowser_v5 CDS 1915949 1916701 . + 0 Rv1691 Rv1691 Function unknown Conserved hypothetical protein Rv1691, MTCI125.13, len: 250 aa. Conserved hypothetical protein, similar to Q9S210|SCI51.30C|AL109848 Hypothetical protein from Streptomyces coelicolor (210 aa), FASTA score: opt: 556, E(): 6.4e-27, (50.6% identity in 180 aa overlap). O33193 No conserved hypotheticals O33193 O33193 NC_000962.3 Mycobrowser_v5 CDS 1916698 1917759 . + 0 Rv1692 Rv1692 Function unknown; probably involved in cellular metabolism Probable phosphatase Rv1692, (MTCI125.14), len: 353 aa. Probable phosphatase, some similarity to others e.g. PNPP_SCHPO|Q00472 4-nitrophenylphosphatase (269 aa), FASTA scores: opt: 214, E(): 1.3e-10, (29.5% identity in 241 aa overlap); and to NAGD_ECOLI|P15302 nagd protein from Escherichia coli (250 aa), FASTA scores: opt: 314, E(): 9.8e-08, (28.2% identity in 245 aa overlap). Also similar to AL109848|SCI51.28 hypothetical protein from Streptomyces coelicolor (343 aa), FASTA scores: opt: 768, E(): 0, (44.8% identity in 315 aa overlap). O33194 No intermediary metabolism and respiration O33194 GO:0008152,GO:0016787 O33194 NC_000962.3 Mycobrowser_v5 CDS 1921542 1922723 . + 0 Rv1697 Rv1697 Function unknown Conserved hypothetical protein Rv1697, (MTCI125.19), len: 393 aa. Conserved hypothetical protein, highly similar to Q49895|MLC1351.11C|U00021 Hypothetical protein of Mycobacterium leprae from cosmid L247 (430 aa), FASTA scores: opt: 2345, E(): 0, (90.6% identity in 393 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O33198 No conserved hypotheticals O33198 O33198 GO:0009229,GO:0004788,GO:0005524 O33198 NC_000962.3 Mycobrowser_v5 CDS 1931497 1932654 . - 0 Rv1705c PPE22 Function unknown PPE family protein PPE22 Rv1705c, (MTCI125.27c), len: 385 aa. PPE22, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, similar to many e.g. YX23_MYCTU|Q10813 hypothetical 41.1 kDa protein cy274.2 3 (404 aa), fasta scores: opt: 819, E(): 0, (46.2% identity in 413 aa overlap). P9WI19 No PE/PPE Q79FL6 P9WI19 P9WI19 NC_000962.3 Mycobrowser_v5 CDS 1918746 1919669 . + 0 Rv1695 ppnK Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [catalytic activity: ATP + NAD+ = ADP + NADP+]. Inorganic polyphosphate/ATP-NAD kinase PpnK (poly(P)/ATP NAD kinase) Rv1695, (MTCI125.17), len: 307 aa. PpnK, inorganic polyphosphate/ATP-NAD kinase (see citation below), equivalent to Q49897|MLC1351.13C|Z95117|PPNK_MYCLE inorganic polyphosphate/ATP-NAD kinase from Mycobacterium leprae (311 aa) (87.9% identity in 305 aa overlap). Also similar to many e.g. P37768|PPNK_ECOLI probable inorganic polyphosphate/ATP-NAD kinase (292 aa), FASTA scores: opt: 384, E(): 1.7e-23, (33.5% identity in 233 aa overlap); etc. Belongs to the NAD kinase family. P9WHV7 No intermediary metabolism and respiration 1U0R,1U0T,1Y3H,1Y3I P0A5S6 P9WHV7 GO:0003951,GO:0005737,GO:0008152,GO:0046872,GO:0005524 2.7.1.23 P9WHV7 NC_000962.3 Mycobrowser_v5 CDS 1919683 1921446 . + 0 Rv1696 recN Involved in recombinational repair of damaged DNA. Probable DNA repair protein RecN (recombination protein N) Rv1696, (MTCI125.18), len: 587 aa. Probable recN, DNA repair protein (see citation below), similar to many e.g. RECN_ECOLI|P05824 dna repair protein recN (553 aa), FASTA scores: opt: 508, E(): 1.9e-33, (31.5% identity in 587 aa overlap). Equivalent to Z95117|MLCB1351_12 recN from Mycobacterium leprae (587 aa), FASTA scores: (76.1% identit y in 589 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). P9WHI7 No information pathways P0A5U6 P9WHI7 GO:0006310,GO:0006281,GO:0005524 P9WHI7 NC_000962.3 Mycobrowser_v5 CDS 1922745 1923689 . + 0 Rv1698 mctB Outer membrane channel Outer membrane protein MctB Rv1698, (MTCI125.20), len: 314 aa. MctB, mycobacterial copper transport protein B essential for Cu resistance and maintenance of low intracellular Cu levels (See Wolschendorf et al., 2011). Outer membrane protein (See Siroy et al., 2008) with predicted N-terminal signal sequence. Probable coiled-coil from aa 31 to 67. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). P9WJ83 No cell wall and cell processes P64883 P9WJ83 GO:0006811,GO:0005886,GO:0046930,GO:0015288,GO:0009279 NC_000962.3 Mycobrowser_v5 CDS 1923829 1925589 . + 0 Rv1699 pyrG Pyrimidine biosynthesis (last step) [catalytic activity: ATP + UTP + glutamine = ADP + orthophosphate + CTP (ammonia can replace glutamine).] Probable CTP synthase PyrG Rv1699, (MTCI125.21), len: 586 aa. Probable pyrG, CTP synthase highly similar to many e.g. PYRG_ECOLI|P08398 ctp synthase from Escherichia coli (544 aa), FASTA scores: opt: 1786, E():0, (51.8% identity in 548 aa overlap). Contains PS00442 Glutamine amidotransferases class-I active site. P9WHK7 No intermediary metabolism and respiration P0A5U2 P9WHK7 GO:0003883,GO:0006541,GO:0006221,GO:0005524 6.3.4.2 P9WHK7 NC_000962.3 Mycobrowser_v5 CDS 1925582 1926205 . + 0 Rv1700 Rv1700 NUDIX hydrolases catalyze the hydrolysis of a variety of nucleoside diphoshate derivatives NUDIX hydrolase Rv1700, (MTCI125.22), len: 207 aa. Nudix hydrolase, equivalent to Q49891|MLC1351.08C|Z95117 Hypothetical protein from Mycobacterium leprae (177 aa), FASTA scores: (66.7% identity in 171 aa overlap); also similar to Q9S225|SCI51.15C|AL109848 Hypothetical protein from Streptomyces coelicolor (211 aa), FASTA scores: opt: 508, E(): 1.2e-27, (43.1% identity in 197 aa overlap); similar to P54570|ADPP_BACSU ADP-ribose pyrophosphatase (185 aa), FASTA scores: opt: 313, E(): 1.1e-06, (42.7% identity in 124 aa overlap). Belongs to the family of Nudix hydrolases I6X235 No information pathways 1MK1,1MP2,1MQE,1MQW,1MR2 O33199 I6X235 GO:0016787 I6X235 NC_000962.3 Mycobrowser_v5 CDS 1926202 1927137 . + 0 Rv1701 Rv1701 Sequence integration/recombination. Probable integrase/recombinase Rv1701, (MTCI125.23), len: 311 aa. Probable integrase/recombinase, similar to many e.g. XERD_ECOLI|P21891 integrase/recombinase xerd (298 aa), FASTA scores: opt: 583, E(): 0, (41.8% identity in 311 aa overlap). Also similar to other Mycobacterium tuberculosis integrase/recombinase proteins RV2894c|MTCY274.25c (43.1% identity in 304 aa overlap); and Rv2646|MTCY441.16 phiRv2 integrase (31.1% identity in 161 aa overlap). Equivalent to Z95117|MLCB1351_7 from Mycobacterium leprae (316 aa) (85.4% identity in 316 aa overlap). P9WF33 No insertion seqs and phages P67636 P9WF33 GO:0015074,GO:0007049,GO:0051301,GO:0007059,GO:0005737,GO:0006313,GO:0009037,GO:0003677 P9WF33 NC_000962.3 Mycobrowser_v5 CDS 1932694 1933878 . - 0 Rv1706c PPE23 Function unknown PPE family protein PPE23 Rv1706c, (MTCI125.28c), len: 394 aa. PPE23, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, similar to many e.g. YX23_MYCTU|Q10813 hypothetical 41.1 kDa protein cy274.23 (404 aa), fasta scores: opt: 841, E(): 3.9e-31, (46.8% identity in 408 aa overlap). P9WI17 No PE/PPE Q7D842 P9WI17 P9WI17 NC_000962.3 Mycobrowser_v5 CDS 1936360 1937316 . + 0 Rv1708 Rv1708 Unknown; thought to be involved in cell process. Putative initiation inhibitor protein Rv1708, (MTCI125.30), len: 318 aa. Putative initiation inhibitor protein, a soj-related protein probably involved in cell process, highly similar to many sporulation initiation inhibitor proteins soj e.g. P37522|SOJ_BACSU Soj protein from Bacillus subtilis (253 aa), FASTA scores: opt: 745, E(): 0, (46.0% identity in 248 aa overlap), and more weakly to various repA/para/incC proteins from various organisms e.g. Y4CK_RHISN|P55393 putative replication protein A from Rhizobium sp. (407 aa), FASTA scores: opt: 205, E(): 4e-13, (29.0% identity in 252 aa overlap). Also similar to Mycobacterium tuberculosis hyothetical proteins Rv3213c and Rv3918c. P9WLT1 No cell wall and cell processes O33207 P9WLT1 GO:0005524 P9WLT1 NC_000962.3 Mycobrowser_v5 CDS 1944406 1944756 . + 0 Rv1717 Rv1717 Function unknown Conserved hypothetical protein Rv1717, (MTCY04C12.02), len: 116 aa. Conserved hypothetical protein, similar to O29060|AF1208|AE001021 Hypothetical protein from Arecheoglobus fulgidus (114 aa), FASTA scores: opt: 254, E(): 3.3e-09, (37.7% identity in 114 aa overlap). O86372 No conserved hypotheticals O86372 O86372 O86372 NC_000962.3 Mycobrowser_v5 CDS 1989042 1989566 . + 0 Rv1758 cut1 Hydrolysis of cutin. Probable cutinase Cut1 Rv1758, (MTCY28.24), len: 174 aa. Probable cut1, serine esterase, cutinase family, similar to Rv2301|CUT2_MYCTU|Q50664 probable cutinase cy339.08c precursor from Mycobacterium tuberculosis (219 aa), FASTA scores: opt: 369, E(): 1. 1e-16, (39.1% identity in 179 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical cutinases Rv3452, Rv1984c, Rv3451 and Rv3724. CDS has been interrupted by IS6110 insertion element and 5'-end deleted. Belongs to the cutinase family. O06793 No cell wall and cell processes O06793 O06793 GO:0008152,GO:0016787 3.1.1.- O06793 NC_000962.3 Mycobrowser_v5 CDS 2282099 2282740 . + 0 Rv2036 Rv2036 Unknown Conserved hypothetical protein Rv2036, (MTV018.23), len: 213 aa. Conserved hypothetical protein; similar to many. O53480 No conserved hypotheticals O53480 O53480 O53480 NC_000962.3 Mycobrowser_v5 CDS 1938146 1938841 . + 0 Rv1710 scpB Involved in chromosome structure and partitioning Possible segregation and condensation protein ScpB Rv1710, (MTCI125.32), len: 231 aa. Possible scpB, segregation and condensation protein, similar to several hypothetical proteins e.g. P35155|YPUH_BACSU from Bacillus subtilis (197 aa), FASTA scores: opt: 339, E(): 1.3e-09, (36.0% identity in 186 aa overlap); Q9S231|SCI51.09C|AL109848 from Streptomyces coelicolor (223 aa), FASTA scores: opt: 626, E(): 0, (51.0% identity in 192 aa overlap). Equivalent to O05669|MLC1351.04C|Z95117 Hypothetical protein from Mycobacterium leprae (231 aa), FASTA scores: (77.9% identity in 231 aa overlap). I6XCB2 No cell wall and cell processes 2Z99 O33209 I6XCB2 GO:0005737,GO:0007049,GO:0051301,GO:0007059 I6XCB2 NC_000962.3 Mycobrowser_v5 CDS 1938838 1939602 . + 0 Rv1711 Rv1711 Function unknown Conserved hypothetical protein Rv1711, (MTCI125.33), len: 254 aa. Conserved hypothetical protein, highly similar to a large family of hypothetical proteins e.g. P37765|YCIL_ECOLI from Escherichia coli (291 aa), FASTA scores: opt: 496, E(): 1.1e-29, (41.6% identity in 250 aa overlap); 9S232|SCI51.08C|AL109848 putative pseudouridine synthase from Streptomyces coelicolor (371 aa), FASTA scores: opt: 818, E(): 0, (53.1% identity in 245 aa overlap). Equivalent to O05668|MLCB1351.03C|Z95117 Hypothetical protein from Mycobacterium leprae (256 aa), (80.5% identity in 256 aa overlap). Contains PS01149 Hypothetical yciL/yejD/yjbC family signature. P9WHQ1 No conserved hypotheticals P65842 P9WHQ1 GO:0009982,GO:0001522,GO:0003723 5.4.99.- P9WHQ1 NC_000962.3 Mycobrowser_v5 CDS 1939599 1940291 . + 0 Rv1712 cmk Catalyzes the transfer of a phosphate group from ATP to either CMP or dCMP to form CDP or dCDP and ADP [catalytic activity: ATP + CMP = ADP + CDP]. Cytidylate kinase Cmk (CMP kinase) (cytidine monophosphate kinase) (ck) Rv1712, (MTCI125.34), len: 230 aa. cmk, cytidylate kinase, highly similar to many e.g. KCY_ECOLI|P23863 cytidylate kinase from Escherichia coli (227 aa), FASTA scores: opt: 534, E (): 0, (40.3% identity in 221 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Equivalent to Z95117|MLCB1351_2 from Mycobacterium leprae (223 aa) (73.5% identity in 226 aa overlap). Belongs to the cytidylate kinase family, subfamily 1. P9WPA9 No intermediary metabolism and respiration P63803 P9WPA9 GO:0004127,GO:0005737,GO:0006220,GO:0005524 2.7.4.14 P9WPA9 NC_000962.3 Mycobrowser_v5 CDS 1940288 1941679 . + 0 Rv1713 engA Binds both GDP and GTP. Has an intrinsic GTPase activity and is essential for cell growth. Probable GTP-binding protein EngA Rv1713, (MTCI125.35), len: 463 aa. Probable engA, GTP-binding protein. Equivalent to Q49884|MLCB1351.01|U00021_5 probable GTP-binding protein ENGA from Mycobacterium leprae (461 aa), (88.6% identity in 463 aa overlap). And similar to many e.g. P50743|ENGA_BACSU probable GTP-binding protein ENGA from Bacillus subtilus (436 aa), FASTA scores: opt: 1077, E(): 0, (40.6% identity in 434 aa overlap). Contains two PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the era/TRME family of GTP-binding proteins. ENGA subfamily. P9WNL3 No intermediary metabolism and respiration P64057 P9WNL3 GO:0005622,GO:0017111,GO:0005525 P9WNL3 NC_000962.3 Mycobrowser_v5 CDS 1941853 1942665 . + 0 Rv1714 Rv1714 Function unknown; probably involved in cellular metabolism Probable oxidoreductase Rv1714, (MTV048.01), len: 270 aa. Probable oxidoreductase similar to many e.g. AE0010|AE001021_4 Archaeoglobus fulgidus section 79 (281 aa), FASTA scores: opt: 578, E(): 3.3e-31, (38.9% identity in 265 aa overlap). Also similar to several other M. tuberculosis oxidoreductases e.g. Rv1544, etc. P9WGQ3 No intermediary metabolism and respiration O53927 P9WGQ3 GO:0055114,GO:0016491 1.-.-.- P9WGQ3 NC_000962.3 Mycobrowser_v5 CDS 1953864 1954634 . - 0 Rv1728c Rv1728c Function unknown Conserved hypothetical protein Rv1728c, (MTCY04C12.13c), len: 256 aa. Conserved hypothetical protein, some similarity to O07246|Rv0320|MTCY63.25 possible exported protein from Mycobacterium tuberculosis (220 aa), FASTA scores: E(): 1.3e-31, (42.3% identity in 220 aa overlap). C-terminal region similar to Q9ZX60|AF068845|AF068845_17 segment of gp17 of Mycobacteriophage TM4 (1229 aa), FASTA scores: opt: 385, E(): 4.3e-17, (44.6% identity in 139 aa overlap). P71986 No conserved hypotheticals P71986 NC_000962.3 Mycobrowser_v5 CDS 1960774 1961016 . - 0 Rv1734c Rv1734c Function unknown Conserved hypothetical protein Rv1734c, (MTCY04C12.19c), len: 80 aa. Conserved hypothetical protein, similar to C-terminal region Q9Z8N2|CP0452|AE001615 Dihydrolipoamide Acetyltransferase from Chlamydia pneumoniae (429 aa), FASTA scores: opt: 138, E(): 0.0012, (26.9% identity in 78 aa overlap). P9WLS7 No conserved hypotheticals P71992 P9WLS7 GO:0008152,GO:0008415 NC_000962.3 Mycobrowser_v5 ncRNA 1952291 1952503 . - 0 MTB000055 AS1726 Unknown Putative small regulatory RNA AS1726, putative small regulatory RNA (See Arnvig and Young, 2009). Alternate 5'-ends at positions 1952400, 1952375, 1952367, and 1952351. No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 1943576 1944406 . + 0 Rv1716 Rv1716 Function unknown Conserved hypothetical protein Rv1716, (MTV048.03,MTCY04C12.01), len: 276 aa. Conserved hypothetical protein, shows high similarity with AF1200|O29068|AE001021_11A conserved protein of Archaeoglobus fulgidus, gp fulgidus section 7 (278 aa), FASTA scores: E(): 0, (61.8% identity in 251 a a overlap); also weak similarity to several polyketide cyclases e.g. O68500|AF048833|DPSY from Streptomyces peucetius (272 aa), FASTA scores: opt: 194, E(): 1.7e-05, (29.6% identity in 223 aa overlap). O53929 No conserved hypotheticals O53929 O53929 O53929 NC_000962.3 Mycobrowser_v5 CDS 1945641 1946420 . + 0 Rv1719 Rv1719 Involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv1719, (MTCY04C12.04), len: 259 aa. Probable transcriptional regulatory protein, similar to YIAJ_ECOLI|P37671 hypothetical transcriptional regulator from Escherichia coli (282 aa), FASTA scores: opt: 353, E(): 3.2e-15, (31.1% identity in 235 aa overlap). Similar to Mycobacterium tuberculosis hypothetical IclR-family transcriptional regulators Rv2989, Rv1773c. Helix-turn-helix motif from aa 34-55 (+6.94 SD). P71977 No regulatory proteins P71977 P71977 GO:0006355,GO:0006350,GO:0003700,GO:0005622 P71977 NC_000962.3 Mycobrowser_v5 CDS 1947030 1947419 . - 0 Rv1720c vapC12 Unknown Possible toxin VapC12 Rv1720c, (MTCY04C12.05c), len: 129 aa. Possible vapC12, toxin, part of toxin-antitoxin (TA) operon with Rv1721c, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to other Mycobacterium tuberculosis hypothetical proteins e.g. O53610|Rv0065|MTV030.08 (133 aa), FASTA scores: E(): 1.5e-10, (39.1% identity in 128 aa overlap); P71550|Rv0960|MTCY10D7.14C (129 aa) and O06415|Rv0549c|MTCY25D10.28C (137 aa). P9WFA3 No virulence, detoxification, adaptation P9WFA3 P9WFA3 NC_000962.3 Mycobrowser_v5 CDS 1949342 1950589 . + 0 Rv1723 Rv1723 Function unknown; probably involved in cellular metabolism Probable hydrolase Rv1723, (MTCY04C12.08), len: 415 aa. Possible hydrolase, similar to others e.g. NYLB_FLASP|P07061 6-aminohexanoate-dimer hydrolase from Flavobacterium sp. (392 aa), FASTA scores: opt: 717, E(): 0, (35.1% identity in 396 aa overlap). Also similar to M. tuberculosis hypothetical esterases and penicillin binding proteins e.g. Rv1923, Rv1497, Rv2463, etc P71981 No intermediary metabolism and respiration P71981 P71981 GO:0016787 3.-.-.- P71981 NC_000962.3 Mycobrowser_v5 CDS 1951852 1953237 . + 0 Rv1726 Rv1726 Function unknown; probably involved in cellular metabolism Probable oxidoreductase Rv1726, (MTCY04C12.11), len: 461 aa. Probable oxidoreductase, similar to HDNO_ARTOX|P08159 6-hydroxy-d-nicotine oxidase (458 aa), FASTA scores: opt: 678, E(): 0, (29.5% identity in 465 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical dehydrogenases e.g. Rv3107c, Rv1257c, etc. P71984 No intermediary metabolism and respiration P71984 P71984 GO:0055114,GO:0016491,GO:0050660 1.-.-.- P71984 NC_000962.3 Mycobrowser_v5 CDS 1953270 1953839 . + 0 Rv1727 Rv1727 Function unknown Conserved hypothetical protein Rv1727, (MTCY04C12.12), len: 189 aa. Conserved hypothetical protein, similar to Mycobacterium tuberculosis hypothetical proteins P72040|Rv3773c|MTCY13D12.07C (194 aa), FASTA scores: opt: 176, E(): 2.7e-08, (31.1% identity in 180 aa overlap); and O53801|Rv0738 (182 aa). P71985 No conserved hypotheticals P71985 P71985 NC_000962.3 Mycobrowser_v5 CDS 1955692 1957245 . - 0 Rv1730c Rv1730c Thought to be involved in cell wall biosynthesis and may also act as a sensor of external penicillins Possible penicillin-binding protein Rv1730c, (MTCY04C12.15c), len: 517 aa. Possible penicillin-binding protein, similar to others e.g. PBP4_NOCLA|Q06317 penicillin-binding protein 4 (pbp-4) from Nocardia lactamdurans (381 aa), FASTA scores: opt: 643, E(): 3.8e-32, (33.8% identity in 370 aa overlap); etc. Also similar to other Mycobacterium tuberculosis hypothetical penicillin binding proteins and esterases e.g. Rv1923, Rv1497, etc. P71988 No cell wall and cell processes P71988 P71988 P71988 NC_000962.3 Mycobrowser_v5 CDS 1959243 1959791 . - 0 Rv1732c Rv1732c Function unknown Conserved protein Rv1732c, (MTCY04C12.17c), len: 182 aa. Conserved protein, highly similar to hypothetical proteins from several organisms e.g. P73178|SLL1289|D90904 from Synechocystis (194 aa), FASTA scores: opt: 663, E(): 0, (53.1% identity in 179 aa overlap); etc. P71990 No conserved hypotheticals P71990 P71990 GO:0045454,GO:0016491,GO:0016209 P71990 NC_000962.3 Mycobrowser_v5 CDS 1959855 1960487 . - 0 Rv1733c Rv1733c Unknown Probable conserved transmembrane protein Rv1733c, (MTCY04C12.18c), len: 210 aa. Probable conserved transmembrane protein. Similar to AL109962|SCJ1_26 hypothetical protein from Streptomyces coelicolor (193 aa), FASTA scores: opt: 287, E(): 3.8e-11, (35.2% identity in 182 aa overlap). Predicted possible vaccine candidate (See Zvi et al., 2008). P9WLS9 No cell wall and cell processes P9WLS9 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2026477 2026776 . + 0 Rv1788 PE18 Function unknown PE family protein PE18 Rv1788, (MTV049.10), len: 99 aa. PE18, Member of the Mycobacterium tuberculosis PE family of gly-, ala-rich proteins (see citation below), similar to Z93777|MTCI364.07 Mycobacterium tuberculosis cosmid (99 aa), FASTA scores: opt: 414, E(): 3.6e-20, (72.4% identity in 98 aa overlap). L7N649 No PE/PPE Q7D7Y9 L7N649 L7N649 NC_000962.3 Mycobrowser_v5 CDS 1965657 1965941 . + 0 Rv1738 Rv1738 Function unknown Conserved protein Rv1738, (MTCY04C12.23), len: 94 aa. Conserved protein, similar to P71931|Rv2632c|YQ32_MYCTU Hypothetical 10.1 kDa protein from Mycobacterium tuberculosis (93 aa), FASTA scores: opt: 319, E(): 2.6e-27, (53.9% identity in 89 aa overlap). Predicted possible vaccine candidate (See Zvi et al., 2008). P9WLS3 No conserved hypotheticals P64887 P9WLS3 P9WLS3 NC_000962.3 Mycobrowser_v5 CDS 1967917 1968165 . + 0 Rv1741 vapC34 Unknown Possible toxin VapC34. Contains PIN domain. Rv1741, (MTCY28.03,MTCY04C12.26), len: 82 aa. Possible vapC34, toxin, part of toxin-antitoxin (TA) operon with Rv1740, contains PIN domain, see Arcus et al. 2005. Similar in N-terminus to others in Mycobacterium tuberculosis e.g. P96914|Rv0624|MTCY20H10.05 (131 aa), (80.4% identity in 56 aa overlap); P71999|Rv1741 (82 aa) and O07769|Rv0609 (133 aa). P9WF71 No virulence, detoxification, adaptation P9WF71 P9WF71 NC_000962.3 Mycobrowser_v5 CDS 1968173 1968910 . + 0 Rv1742 Rv1742 Unknown Unknown protein Rv1742, (MTCY28.04,MTCY04C12.27), len: 245 aa. Unknown protein. O33271 No unknown O33271 NC_000962.3 Mycobrowser_v5 CDS 1986854 1987696 . - 0 Rv1755c plcD Hydrolyzes sphingomyelin in addition to phosphatidylcholine. Probable virulence factor implicated in the pathogenesis of M.tuberculosis at the level of intracellular survival, by the alteration of cell signaling events or by direct cytotoxicity [catalytic activity: a phosphatidylcholine + H(2)O = 1,2- diacylglycerol + choline phosphate]. Probable phospholipase C 4 (fragment) PlcD Rv1755c, (MT1799, MTCY28.21c), len: 280 aa. Probable plcD, phospholipase C 4 (fragment) (see citations below), highly similar to C-terminus of other phospholipases e.g. CQ50771|Rv2351c|PLCA|MTP40|MT2416|MTCY98.20c phospholipase C 1 from Mycobacterium tuberculosis (512 aa), FASTA score: (71.1% identity in 284 aa overlap); etc. Note that this ORF has been interrupted by insertion of IS6110 element. Belongs to the bacterial phospholipase C family. P9WIA9 No intermediary metabolism and respiration P0A5R8 P9WIA9 GO:0034480 3.1.4.3 P9WIA9 NC_000962.3 Mycobrowser_v5 CDS 1987745 1988731 . - 0 Rv1756c Rv1756c Required for the transposition of the insertion element IS6110. Putative transposase Rv1756c, (MTCY28.22c), len: 328 aa. Putative Transposase subunit for IS6110. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv1756c and Rv1757c, the sequence UUUUAAAG (directly upstream of Rv1756c) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 34 aa) P9WKH9 No insertion seqs and phages P0C5G8 P9WKH9 GO:0015074,GO:0006310,GO:0032196,GO:0003677 P9WKH9 NC_000962.3 Mycobrowser_v5 CDS 1999737 2000006 . + 0 Rv1766 Rv1766 Function unknown Conserved protein Rv1766, (MTCY28.32), len: 89 aa. Conserved protein, highly similar to P54431|YRKD_BACSU Hypothetical 7.0 kDa protein in bltr-spoIIIC intergenic region from Bacillus subtilis (63 aa), FASTA scores: opt: 151, E(): 1.5e-05, (53.3% identity in 45 aa overlap). Also similar to Q9RD62|SCF56.04C|AL133424 Hypothetical protein from Streptomyces coelicolor (92 aa), FASTA scores: opt: 239, E(): 1.3e-11, (62.5% identity in 64 aa overlap). Also some similarity to other Mycobacterium tuberculosis hypothetical proteins e.g. O07434|Rv0190|MTCI28.29 (96 aa), (35.5% identity in 62 aa overlap); P71543|Rv0967 (119 aa), and P71600|Rv0030 (109 aa). Start changed since original submission. O06799 No conserved hypotheticals O06799 O06799 O06799 NC_000962.3 Mycobrowser_v5 CDS 2000614 2002470 . + 0 Rv1768 PE_PGRS31 Function unknown PE-PGRS family protein PE_PGRS31 Rv1768, (MTCY28.34), len: 618 aa. PE_PGRS31, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), highly similar to Q50615 hypothetical 40.8 kDa protein (498 aa), FASTA scores: opt: 1703, E(): 0, (57.4% identity in 566 aa overlap). Q79FK9 No PE/PPE Q79FK9 Q79FK9 Q79FK9 NC_000962.3 Mycobrowser_v5 CDS 1970989 1971390 . - 0 Rv1744c Rv1744c Unknown Probable membrane protein Rv1744c, (MTCY28.06c), len: 133 aa. Probable membrane protein, contains four imperfect 10 aa repeats, some similarity to Q25946 (MSA-2) (fragment) from Plasmodium falciparum (205 aa), FASTA scores: opt: 145, E( ): 0.048, (52.4% identity in 63 aa overlap). O06787 No cell wall and cell processes O06787 O06787 NC_000962.3 Mycobrowser_v5 CDS 1971380 1971991 . - 0 Rv1745c idi Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopenten to its allylic isomer, dimethylallyl diphosphate (DMAPP) [catalytic activity :isopentenyl diphosphate = dimethylallyl diphosphate] Probable isopentenyl-diphosphate delta-isomerase Idi (IPP isomerase) (isopentenyl pyrophosphate isomerase) Rv1745c, (MTCY28.08c,MTCY04C12.29c), len: 203 aa. Probable idi, isopentenyl-diphosphate delta-isomerase, similar to Q46822|ORF_O182 from Escherichia coli (182 aa), FASTA scores: opt: 465, E(): 4.7e-25, (46.9% identity in 162 aa overlap), and to IPPI_SCHPO|Q10132 isopentenyl-diphosphate delta-isomerase from Schizosaccharomyces pombe (227 aa), FASTA scores: opt: 185, E(): 5.4e-06, (30.3% identity in 152 aa overlap). Belongs to the IPP isomerase type 1 family. P9WKK5 No intermediary metabolism and respiration P72002 P9WKK5 GO:0016787,GO:0004452,GO:0008299,GO:0046872,GO:0005737 5.3.3.2 P9WKK5 NC_000962.3 Mycobrowser_v5 CDS 1972138 1973568 . + 0 Rv1746 pknF Involved in signal transduction (via phosphorylation). Thought to be involved in membrane transport. Phosphorylates the peptide substrate myelin basic protein (MBP) at serine and threonine residues [catalytic activity: ATP + a protein = ADP + a phosphoprotein]. Anchored-membrane serine/threonine-protein kinase PknF (protein kinase F) (STPK F) Rv1746, (MTCY28.09, MTCY04C12.30), len: 476 aa. pknF, transmembrane serine/threonine-protein kinase (see citations below), highly similar to KY28_MYCTU|Q10697 probable serine/threonine-protein kinase from Mycobacterium tuberculosis (589 aa), FASTA scores: opt: 870, E(): 0, (41.6% identity in 406 aa overlap). Contains PS00108 Serine/Threonine protein kinases active-site signature. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases. Experimental studies show evidence of auto-phosphorylation. Start site chosen by homology, may extend further upstream. P9WI75 No regulatory proteins P72003 P9WI75 GO:0006468,GO:0004674,GO:0005524 2.7.11.1 P9WI75 NC_000962.3 Mycobrowser_v5 CDS 1973630 1976227 . + 0 Rv1747 Rv1747 Thought to be involved in active transport of undetermined substrate (possibly lipooligosaccharide) across the membrane. Responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane. Probable conserved transmembrane ATP-binding protein ABC transporter Rv1747, (MTCY28.10, MTCY04C12.31), len: 865 aa. Probable conserved transmembrane ATP-binding protein ABC transporter (see citation below), similar to others e.g Q55956 ABC transporter from Synechocystis sp. (790 aa), FASTA scores: opt: 738, E(): 6.3e-26, (31.6% identity in 632 aa overlap); etc. Also similar to other M. tuberculosis ABC-type transporters e.g. Rv2397c|MTCY253.24, FASTA score: (35.2% identity in 213 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). O65934 No cell wall and cell processes O65934 O65934 GO:0016887,GO:0016021,GO:0005524 O65934 NC_000962.3 Mycobrowser_v5 CDS 1976600 1977331 . + 0 Rv1748 Rv1748 Unknown Unknown protein Rv1748, (MTCY28.11, MTCY04C12.32), len: 243 aa. Unknown protein. Possibly exported protein, hydrophobic domain, TM helix aa 23-45. P72005 No conserved hypotheticals P72005 NC_000962.3 Mycobrowser_v5 CDS 2881758 2882147 . + 0 Rv2562 Rv2562 Function unknown Conserved hypothetical protein Rv2562, (MTCY9C4.06c), len: 129 aa. Conserved hypothetical protein, highly similar, but shorter 83 aa, to downstream ORF AAK46951|RV2561|MT2638|MTCY9C4.07c conserved hypothetical protein from Mycobacterium tuberculosis (97 aa), FASTA scores: opt: 866, E(): 2.2e-54, (100.0% identity in 129 aa overlap). Q50737 No conserved hypotheticals Q50737 Q50737 P9WL99 NC_000962.3 Mycobrowser_v5 CDS 1979621 1981003 . + 0 Rv1751 Rv1751 Function unknown; probably involved in cellular metabolism Probable oxidoreductase Rv1751, (MTCY28.14-MTCY04C12.35), len: 460 aa. Probable oxidoreductase, possibly a monooxygenase or hydroxylase, similar to MHPA_ECOLI|P77397 3-(3-hydroxy-phenyl) propionate hydroxylase (554 aa), FASTA scores: opt: 239, E(): 2e-08, (24.6% identity in 435 aa overlap); and AJ007932|SAR7932.13 oxygenase from Streptomyces argillaceus (436 aa), FASTA scores: opt: 587, E(): 8.6e-30, (32.3% identity in 359 aa overlap). Contains PS00075 Dihydrofolate reductase signature. Also similar to Mycobacterium tuberculosis hypothetical oxidoreductases Rv1260 and Rv0575c. O65936 No intermediary metabolism and respiration O65936 O65936 GO:0055114,GO:0004497 1.-.-.- O65936 NC_000962.3 Mycobrowser_v5 CDS 1981614 1984775 . - 0 Rv1753c PPE24 Function unknown PPE family protein PPE24 Rv1753c, (MTCY28.16c), len: 1053 aa. PPE24, Member of the Mycobacterium tuberculosis PPE family of Gly-, Asn-rich proteins, similar to many e.g. YF48_MYCTU|Q10778 hypothetical protein cy48.17 (678 aa), FASTA scores: opt: 1360, E(): 0, (48.9% identity in 550 aa overlap). Note that the Gly-, Asn-rich sequence is interrupted by six near-perfect 26 aa repeats, a unique region, and another, more degenerate region of five 25 aa repeats before resuming at the C-terminus. The end of the first Gly-, Asn- rich region and the start of the first set of repeats shows some similarity to Q50577|AT10S from Mycobacterium tuberculosis (170 aa) (40.2% identity in 189 aa overlap). P9WI15 No PE/PPE Q79FL2 P9WI15 P9WI15 NC_000962.3 Mycobrowser_v5 CDS 1984979 1986670 . - 0 Rv1754c Rv1754c Function unknown Conserved protein Rv1754c, (MTCY28.17c), len: 563 aa. Conserved protein, has proline-rich central region. Some similarity in central region to other Mycobacterium tuberculosis proline-rich proteins e.g. O06555|Rv1157c|MTCI65.24c (371 aa), (32.5% identity in 191 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06790 No conserved hypotheticals O06790 O06790 NC_000962.3 Mycobrowser_v5 CDS 2003878 2005164 . + 0 Rv1770 Rv1770 Function unknown Conserved protein Rv1770, (MTCY28.36), len: 428 aa. Conserved protein, highly similar in N-terminus to Q49882 Hypothetical protein from Mycobacterium leprae from cosmid L247 (83 aa), FASTA scores: opt: 301, E(): 1e-12, (56.5% identity in 85 aa overlap). O06803 No conserved hypotheticals O06803 O06803 GO:0006508,GO:0008233 NC_000962.3 Mycobrowser_v5 CDS 1993153 1994661 . + 0 Rv1760 Rv1760 May be involved in synthesis of triacylglycerol Possible triacylglycerol synthase (diacylglycerol acyltransferase) Rv1760, (MTCY28.26), len: 502 aa. Possible triacylglycerol synthase (See Daniel et al., 2004), similar to several other Mycobacterium tuberculosis proteins e.g. Q10554|Y895_MYCTU|MTCY31.23 (505 aa), FASTA scores: opt: 692, E(): 0, (31.7% identity in 477 aa overlap). Member of family with at least 15 other members e.g. Rv3740c, Rv3734c, Rv1425, etc. P9WKB9 No lipid metabolism O06795 P9WKB9 GO:0006071,GO:0008610,GO:0004144 2.3.1.20 NC_000962.3 Mycobrowser_v5 CDS 1996152 1996478 . + 0 Rv1763 Rv1763 Required for the transposition of the insertion element IS6110. Putative transposase for insertion sequence element IS6110 (fragment) Rv1763, (MTCY28.29), len: 108 aa. Putative Transposase for IS6110 (fragment), identical to many other Mycobacterium tuberculosis IS6110 transposase subunits e.g. Q50686|YIA4_MYCTU Insertion element IS6110 hypothetical 12.0 kDa protein (108 aa), fasta scores: E(): 1.4e-43, (100.00% identity in 108 aa overlap). The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv1763 and Rv1764, the sequence UUUUAAAG (directly upstream of Rv1764) maybe responsible for such a frameshifting event (see McAdam et al., 1990). P9WKH5 No insertion seqs and phages P0C5G9 P9WKH5 GO:0004803,GO:0006313,GO:0003677 P9WKH5 NC_000962.3 Mycobrowser_v5 CDS 1997418 1998515 . - 0 Rv1765c Rv1765c Function unknown Conserved hypothetical protein Rv1765c, (MTCY28.31c), len: 365 aa. Conserved hypothetical protein, highly similar to O53461|Rv2015c|MTV018.02c conserved hypothetical protein (418 aa), (97.8% identity in 364 aa overlap). Blast hits with non-is part of sequence submitted under MTU78639. O06798 No conserved hypotheticals O06798 O06798 GO:0003676,GO:0004519 NC_000962.3 Mycobrowser_v5 CDS 2007020 2007766 . - 0 Rv1773c Rv1773c Involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv1773c, (MTCY28.39), len: 248 aa. Probable transcriptional regulator belonging to IclR family, similar to ICLR_ECOLI|P16528 acetate operon repressor from Escherichia coli (274 aa), FASTA scores: opt: 261, E(): 3.3e-10, (26.9% identity in 249 aa overlap). Also similar to Mycobacterium tuberculosis protein Rv1719|MTCY04C12.04 (40.2% identity in 244 aa overlap); and Rv2989. Start site chosen by homology, but may extend further upstream. Contains possible helix-turn-helix motif at aa 37-58 (+3.24 SD). O06806 No regulatory proteins O06806 O06806 GO:0006355,GO:0006350,GO:0003677 O06806 NC_000962.3 Mycobrowser_v5 CDS 2007832 2009172 . + 0 Rv1774 Rv1774 Function unknown; probably involved in cellular metabolism Probable oxidoreductase Rv1774, (MTCY25C11.01), len: 446 aa. Probable oxidoreductase, similar to several e.g. HDNO_ARTOX|P08159 6-hydroxy-d-nicotine oxidase (458 aa), FASTA scores: opt: 417, E(): 6e-20, (28.4% identity in 462 aa overlap). Also some similarity to Mycobacterium tuberculosis oxidoreductase MTCY04C12.11 (24.1% identity in 444 aa overlap). Contains PS00862 Oxygen oxidoreductases covalent FAD-binding site. O33177 No intermediary metabolism and respiration O33177 O33177 GO:0055114,GO:0016491,GO:0050660 1.-.-.- O33177 NC_000962.3 Mycobrowser_v5 CDS 2009995 2010555 . - 0 Rv1776c Rv1776c Involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv1776c, (MTCY25C11.03c), len: 186 aa. Possible regulatory protein, some similarity to Mycobacterium tuberculosis Rv1255c|Q11063 hypothetical transcriptional regulator (202 aa), FASTA scores: opt: 270, E(): 9.7e-09, (28.3% identity in 191 aa overlap). Contains possible helix-turn-helix motif at aa 37-58 (+3.49 SD). O33179 No regulatory proteins O33179 O33179 GO:0006350,GO:0003700,GO:0006355 O33179 NC_000962.3 Mycobrowser_v5 CDS 2012686 2014479 . - 0 Rv1779c Rv1779c Unknown Possible integral membrane protein Rv1779c, (MTV049.01c), len: 597 aa. Possible integral membrane protein. O53930 No cell wall and cell processes O53930 O53930 NC_000962.3 Mycobrowser_v5 CDS 2014699 2015262 . + 0 Rv1780 Rv1780 Function unknown Conserved protein Rv1780, (MTV049.02), len: 187 aa. Conserved protein, equivalent to Q49881|ML1380|U00021_2 cosmid L247 from Mycobacterium leprae (187 aa), FASTA scores: opt: 1000, E(): 0, (82.4% identity in 187 aa overlap). O53931 No conserved hypotheticals O53931 NC_000962.3 Mycobrowser_v5 CDS 2048398 2049597 . + 0 Rv1807 PPE31 Function unknown PPE family protein PPE31 Rv1807, (MTV049.29), len: 399 aa. PPE31, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, most similar to Rv1789|MTV049.11|AL022021 (393 aa), FASTA scores: opt: 1169, E(): 0, (49.5% identity in 412 aa overlap). L0T7Y7 No PE/PPE Q79FJ7 L0T7Y7 L0T7Y7 NC_000962.3 Mycobrowser_v5 CDS 2024828 2025031 . + 0 Rv1786 Rv1786 Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Probable ferredoxin Rv1786, (MTV049.08), len: 67 aa. Probable ferredoxin, similar to others e.g. X63601|FERS_STRGR ferredoxin from Streptomyces griseus (65 aa), FASTA scores: opt: 140, E(): 0.001, (38.1% identity in 63 aa overlap); T50943 probable ferredoxin DitA from Pseudomonas abietaniphila (78 aa); BAA84714.1|AB017795 ferredoxin from Nocardioides sp. (69 aa); etc. Also similar to Rv0763c|MTCY369.08 from Mycobacterium tuberculosis (68 aa), FASTA score: (30.6% identity in 62 aa overlap); and Rv0763c. O53937 No intermediary metabolism and respiration O53937 GO:0016491,GO:0055114 1.-.-.- O53937 NC_000962.3 Mycobrowser_v5 CDS 2017740 2019260 . + 0 Rv1782 eccB5 Function unknown ESX conserved component EccB5. ESX-5 type VII secretion system protein. Probable membrane protein. Rv1782, (MTV049.04), len: 506 aa. eccB5, esx conserved component, ESX-5 type VII secretion system protein, probable membrane protein, similar to four other Mycobacterium tuberculosis hypothetical membrane proteins e.g. O05449|Rv3895c|MTCY15F10.17|Z94121 (495 aa), FASTA scores: opt: 1106, E(): 0, (41.2% identity in 485 aa overlap); Rv0283, Rv3450c, and Rv3869, all located near ESAT-6 family genes. Also similar to O33088|MLCB628.17C|Y14967 cosmid B628 from Mycobacterium leprae (481 aa), (32.7% identity in 486 aa overlap); and equivalent to Q9Z5I3|MLCB596.27|AL035472 hypothetical protein from Mycobacterium leprae (506 aa) (82.6% identity in 506 aa overlap). Has hydrophobic stretch from aa 54-76. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WNQ9 No cell wall and cell processes O53933 P9WNQ9 GO:0005886,GO:0016021 P9WNQ9 NC_000962.3 Mycobrowser_v5 CDS 2019257 2023432 . + 0 Rv1783 eccC5 Function unknown ESX conserved component EccC5. ESX-5 type VII secretion system protein. Rv1783, (MTV049.05-MTV049.06), len: 1391 aa. eccC5, esx conserved component, ESX-5 type VII secretion system protein, probable membrane protein. FtsK/SpoIIIE family protein. Similar to Rv3894c. Member of family of Mycobacterium tuberculosis hypothetical proteins including Rv3447c, Rv0284, Rv3870, Rv1783, Rv3871, Rv3894c, all linked to ESAT-6 family genes. Equivalent to Mycobacterium leprae hypothetical protein Q9Z512|MLCB596.28|AL035472 (1345 aa). Previously annotated as two separate genes eccCa5|Rv1783 and eccCb5|Rv1784, now fused due to A:T correction at position 2020563 resulting in *463L. Contains two times PS00017 ATP/GTP-binding site motif A (P-loop). Former Rv1784 - Predicted to be an outer membrane protein (See Song et al., 2008). P9WNA5 No cell wall and cell processes O53935,O53934 P9WNA5 GO:0005886,GO:0016021,GO:0003677,GO:0007049,GO:0051301,GO:0007059,GO:0005737,GO:0017111,GO:0005524 P9WNA5 NC_000962.3 Mycobrowser_v5 CDS 2023447 2024628 . - 0 Rv1785c cyp143 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Probable cytochrome P450 143 Cyp143 Rv1785c, (MT1834, MTV049.07c), len: 393 aa. Probable cyp143, cytochrome P450 (1.14.-.-), similar to many e.g. AE0001|RZAE000101_4 Rhizobium sp. NGR234 (414 aa), FASTA scores: opt: 663, E(): 0, (32.4% identity in 413 aa overlap). Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. Belongs to the cytochrome P450 family. P9WPL3 No intermediary metabolism and respiration P63723 P9WPL3 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPL3 NC_000962.3 Mycobrowser_v5 CDS 2025301 2026398 . + 0 Rv1787 PPE25 Function unknown PPE family protein PPE25 Rv1787, (MTV049.09), len: 365 aa. PPE25, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, similar to Z74024|MTCY274.24 Mycobacterium tuberculosis cosmid (404 aa), FASTA scores: opt: 837, E(): 0, (52.0% identity in 406 aa overlap). P9WI13 No PE/PPE Q79FK8 P9WI13 P9WI13 NC_000962.3 Mycobrowser_v5 CDS 2026790 2027971 . + 0 Rv1789 PPE26 Function unknown PPE family protein PPE26 Rv1789, (MTV049.11), len: 393 aa. PPE26, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, highly similar to others e.g.Z98268|MTCI125.26 Mycobacterium tuberculosis cosmid (385 aa), FASTA score: opt: 1283, E(): 0, (62.7% identity in 408 aa overlap). Q79FK6 No PE/PPE Q79FK6 Q79FK6 Q79FK6 NC_000962.3 Mycobrowser_v5 CDS 2030347 2030643 . + 0 Rv1792 esxM Function unknown ESAT-6 like protein EsxM Rv1792, (MTV049.14), len: 98 aa. EsxM, ESAT-6 like protein (see Gey Van Pittius et al., 2001), member of Mycobacterium tuberculosis QILSS family of proteins with Rv1038c, Rv1197, Rv3620c and Rv2347c. Has in-frame stop codon at 18074, no error could be found to account for this. Identical (apart from stop codon) to P96363|Rv1038c|MTCY10G2.11 putative ESAT-6 like protein 2 (98 aa), FASTA scores: opt: 389, E(): 5.8e-26, (100.0% identity in 58 aa overlap). Similar protein present in Mycobacterium leprae e.g. Q49946|MLCB1701.06C|AL049191 putative ESAT-6 like protein X (95 aa), FASTA scores: opt: 343, E(): 1.6e-17, (57.6% identity in 92 aa overlap). Seems to belong to the ESAT6 family. Yes cell wall and cell processes NC_000962.3 Mycobrowser_v5 CDS 2029904 2030203 . + 0 Rv1791 PE19 Function unknown PE family protein PE19 Rv1791, (MTV049.13), len: 99 aa. PE19, Member of the Mycobacterium tuberculosis PE family, but no glycine rich C-terminus (see Brennan & Delogu 2002), highly similar to Z93777|MTCI364.07 M.tuberculosis cosmid (99 aa) opt: 430 E(): 2.4e-21, (75.5% identity in 98 aa overlap). Q79FK4 No PE/PPE Q79FK4 Q79FK4 Q79FK4 NC_000962.3 Mycobrowser_v5 CDS 2030694 2030978 . + 0 Rv1793 esxN Function unknown Putative ESAT-6 like protein EsxN (ESAT-6 like protein 5) Rv1793, (MT1842, MTV049.15), len: 94 aa. EsxN, ESAT-6 like protein (see citation below), almost identical to several mycobacterial proteins of the ESAT-6-like family including P95242|Rv2346c|MTCY98.15C|Z83860 putative ESAT-6 like protein 6 (94 aa), FASTA scores: opt: 610, E(): 0, (97.9 % identity in 94 aa overlap); Rv3619c, Rv1037c, and Rv1198, etc. Also present in Mycobacterium leprae. Seems to belong to the ESAT6 family. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WNJ3 No cell wall and cell processes P9WNJ3 P9WNJ3 NC_000962.3 Mycobrowser_v5 CDS 2032240 2033751 . + 0 Rv1795 eccD5 Unknown ESX conserved component EccD5. ESX-5 type VII secretion system protein. Probable membrane protein. Rv1795, (MTV049.17), len: 503 aa. eccD5, esx conserved component, ESX-5 type VII secretion system protein, probable membrane protein, has a hydrophilic stretch from ~1-130 then very hydrophobic. Similar to several other mycobacterial proteins, all linked to ESAT-6 family e.g. Rv3887c|MTY15F10.24|Z94121 (509 aa), FASTA scores: opt: 360, E(): 1.6e-15, (26.7% identity in 514 aa overlap); Rv3448, and Rv0290. P9WNP9 No cell wall and cell processes O53944 P9WNP9 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2052933 2053289 . + 0 Rv1810 Rv1810 Function unknown Conserved protein Rv1810, (MTCY16F9.04c), len: 118 aa. Conserved protein, similar to several hypothetical Mycobacterium tuberculosis proteins that may be exported (possible N-terminal signal sequence) e.g. O53953|Rv1804c|MTV049.26c|AL022021 (108 aa), FASTA scores: opt: 361, E(): 9.6e-17, (53.5% identity in 101 aa overlap); Rv0622, and Rv1690, etc. O07222 No conserved hypotheticals O07222 O07222 O07222 NC_000962.3 Mycobrowser_v5 CDS 2057528 2058193 . + 0 Rv1815 Rv1815 Function unknown Conserved protein Rv1815, (MTCY1A11.28c), len: 221 aa. Conserved protein, similar to G473456 hypothetical protein from Mycobacterium fortuitum (255 aa), FASTA scores: opt: 182, E(): 3.2e-05, (29.6% identity in 230 aa overlap). Alternative nucleotide at position 2057774 (a->T; I83F) has been observed. Predicted to be an outer membrane protein (See Song et al., 2008). P9WLR9 No conserved hypotheticals P9WLR9 GO:0003824 P9WLR9 NC_000962.3 Mycobrowser_v5 CDS 2039159 2039350 . + 0 Rv1799 lppT Unknown Probable lipoprotein LppT Rv1799, (MTV049.21), len: 63 aa. Probable lppT lipoprotein, has possible signal peptide and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O53948 No cell wall and cell processes O53948 NC_000962.3 Mycobrowser_v5 CDS 2039453 2041420 . + 0 Rv1800 PPE28 Function unknown PPE family protein PPE28 Rv1800, (MTV049.22), len: 655 aa. PPE28, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, C-terminal very similar to parts of PE proteins e.g. Z92770|MTCI5.25|Rv0151c (588 aa), FASTA scores: opt: 1269, E(): 0, (41.5% identity in 591 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). P9WI11 No PE/PPE Q79FK2 P9WI11 P9WI11 NC_000962.3 Mycobrowser_v5 CDS 2042001 2043272 . + 0 Rv1801 PPE29 Function unknown PPE family protein PPE29 Rv1801, (MTV049.23), len: 423 aa. PPE29, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, most similar to AL022021|MTV049.29|Rv1808 (409 aa), FASTA scores: opt: 1229, E(): 0, (55.2% identity in 422 aa overlap). P9WI09 No PE/PPE Q7D7X9 P9WI09 P9WI09 NC_000962.3 Mycobrowser_v5 CDS 2043384 2044775 . + 0 Rv1802 PPE30 Function unknown PPE family protein PPE30 Rv1802, (MTV049.24), len: 463 aa. PPE30, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, most similar to AL022021|MTV049.30|Rv1809 (468 aa), FASTA scores: opt: 1238, E(): 0, (51.0% identity in 471 aa overlap). P9WI07 No PE/PPE P0A692 P9WI07 P9WI07 NC_000962.3 Mycobrowser_v5 CDS 2049921 2051150 . + 0 Rv1808 PPE32 Function unknown PPE family protein PPE32 Rv1808, (MTV049.30), len: 409 aa. PPE32, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, most similar to Rv1800|MTV049.22|AL022021 (655 aa), FASTA scores: opt: 1225, E(): 0, (55.1% identity in 423 aa overlap). Contains PS00343 Gram-positive cocci surface proteins 'anchoring' hexapeptide. Nucleotide position 2050913 in the genome sequence has been corrected, A:G resulting in E331E. P9WI05 No PE/PPE Q79FJ6 P9WI05 P9WI05 NC_000962.3 Mycobrowser_v5 CDS 2055681 2056112 . - 0 Rv1813c Rv1813c Function unknown Conserved hypothetical protein Rv1813c, (MTCY16F9.01), len: 143 aa. Conserved hypothetical protein. Possibly a exported protein with potential N-terminal signal sequence. Similar to Q11050|Rv1269c|MTCY50.13 hypothetical protein from Mycobacterium tuberculosis (124 aa), (42.7% identity in 143 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). Predicted possible vaccine candidate (See Zvi et al., 2008). P9WLS1 No conserved hypotheticals P9WLS1 NC_000962.3 Mycobrowser_v5 CDS 2047023 2047349 . - 0 Rv1804c Rv1804c Function unknown Conserved protein Rv1804c, (MTV049.26c), len: 108 aa. Conserved protein, similar to several hypothetical Mycobacterium tuberculosis proteins that may be exported (hydrophobic stretch at N-terminus) e.g. O07222|Rv1810|MTCY16F9.04C|Z96073 (118 aa), FASTA scores: opt: 361, E(): 2.3e-19, (53.5% identity in 101 aa overlap); Rv0622, Rv1690, and Rv3067, etc. Predicted to be an outer membrane protein (See Song et al., 2008). O53953 No conserved hypotheticals O53953 O53953 NC_000962.3 Mycobrowser_v5 CDS 2047687 2048034 . - 0 Rv1805c Rv1805c Unknown Hypothetical protein Rv1805c, (MTV049.27c), len: 115 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53954 No conserved hypotheticals O53954 NC_000962.3 Mycobrowser_v5 CDS 2048072 2048371 . + 0 Rv1806 PE20 Function unknown PE family protein PE20 Rv1806, (MTV049.28), len: 99 aa. PE20, Member of the Mycobacterium tuberculosis PE family of gly-, ala-rich proteins (see citation below), most similar to Rv1788|MTV049.10|AL022021 (99 aa), FASTA scores: opt: 334, E(): 4.7 e-15, (59.8% identity in 97 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L7N656 No PE/PPE Q7D7X6 L7N656 L7N656 NC_000962.3 Mycobrowser_v5 CDS 2053443 2054147 . + 0 Rv1811 mgtC Thought to be involved in Mg2+ transport (import). May act as an accessory protein for MGTB so mediating magnesium influx into the cytosol [catalytic activity: ATP + H(2)O + Mg(2+)(out) = ADP + phosphate + Mg(2+)(in)]. Possible Mg2+ transport P-type ATPase C MgtC Rv1811, (MTCY16F9.03c), len: 234 aa. Possible mgtC, magnesium (Mg2+) transport P-type ATPase C (transmembrane protein), highly similar to many e.g. NP_442124.1|NC_000911 Mg2+ transport ATPase from Synechocystis sp. strain PCC 6803 (234 aa); NP_251248.1|NC_002516 probable transport protein from Pseudomonas aeruginosa (230 aa); P22037|ATMC_SALTY|STM3764 magnesium transport ATPase protein C from Salmonella typhimurium (231 aa), FASTA scores: opt: 545, E(): 4.1e-30, (42.3% identity in 220 aa overlap); N-terminus of NP_213315.1|NC_000918 Mg(2+) transport ATPase from Aquifex aeolicus (225 aa); etc. Belongs to the MGTC / SAPB family I6YBN6 No cell wall and cell processes O07221 I6YBN6 GO:0004012,GO:0016020 3.6.3.1 I6YBN6 NC_000962.3 Mycobrowser_v5 CDS 2054157 2055359 . - 0 Rv1812c Rv1812c Function unknown; probably involved in cellular metabolism Probable dehydrogenase Rv1812c, (MTCY16F9.02), len: 400 aa. Probable dehydrogenase, similar to other dehydrogenases/oxidases e.g. AE001947|AE001947_10 NADH dehydrogenase II of Deinococcus radiodurans (379 aa), FASTA scores: opt: 404, E(): 3.4e-18, (26.4% identity in 363 aa overlap) and DHNA_HAEIN|P44856 nadh dehydrogenase (444 aa), FASTA scores: opt: 200, E(): 8.5e-06, (23.3% identity in 258 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical dehydrogenases Rv0392c, and Rv1854c|MTCY359.19 ndh probable NADH dehydrogenase (31.5% identity in 321 aa overlap). P9WJJ1 No intermediary metabolism and respiration O07220 P9WJJ1 GO:0055114,GO:0016491,GO:0050660 1.6.-.- P9WJJ1 NC_000962.3 Mycobrowser_v5 CDS 2058256 2058960 . + 0 Rv1816 Rv1816 Involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv1816, (MTCY1A11.27c), len: 234 aa. Possible transcriptional regulatory protein. MEME analysis suggests similarity to putative Mycobacterium tuberculosis transcriptional regulators, Rv0653c, Rv0681. Contains helix-turn-helix motif at aa 38-59 (+4.30 SD). P9WMC9 No regulatory proteins P67438 P9WMC9 GO:0006355,GO:0006350,GO:0003700,GO:0016021 P9WMC9 NC_000962.3 Mycobrowser_v5 CDS 2059595 2061058 . + 0 Rv1817 Rv1817 Function unknown; probably involved in cellular metabolism Possible flavoprotein Rv1817, (MTCY1A11.26c), len: 487 aa. Possible flavoprotein, similar to G746486 flavoprotein subunit of fumarate reductase FAD domain homologue (474 aa), FASTA scores: opt: 223, E(): 5.7e-07, (24.1% identity in 489 aa overlap); and AJ236923|SFR236923_3 soluble fumarate reductase of Shewanella frigidimarina ifcA (588 aa), FASTA scores: opt: 310, E(): 2.5e-11, (27.3% identity in 484 aa overlap). Q50616 No intermediary metabolism and respiration Q50616 Q50616 GO:0016491,GO:0009055 Q50616 NC_000962.3 Mycobrowser_v5 CDS 2061178 2062674 . - 0 Rv1818c PE_PGRS33 Function unknown. Seems to influence both cell surface interactions among mycobacteria and the interactions of bacteria with macrophages. PE-PGRS family protein PE_PGRS33 Rv1818c, (MTCY1A11.25), len: 498 aa. PE_PGRS33, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins, similar to many. Contains 2 x PS00583 pfkB family of carbohydrate kinases signature 1. Supposedly localised to the cell surface (see citations below). P9WIF5 No PE/PPE Q50615 P9WIF5 GO:0005886,GO:0016021 P9WIF5 NC_000962.3 Mycobrowser_v5 CDS 2064799 2066442 . + 0 Rv1820 ilvG Valine and isoleucine biosynthesis (first step) [catalytic activity: 2-acetolactate + CO(2) = 2 pyruvate] Probable acetolactate synthase IlvG (acetohydroxy-acid synthase)(ALS) Rv1820, (MTCY1A11.23c), len: 547 aa. Probable ilvG, acetolactate synthase. Equivalent to AL008609|MLCB1788.46c ilvG from Mycobacterium leprae (548 aa) (86.1% identity in 548 aa overlap). Similar to ILVB_KLEPN|P27696 (559 aa), FASTA scores: opt: 660, E(): 2.9e-34, (29.1% identity in 549 aa overlap). Also similar to other Mycobacterium tuberculosis Ilv proteins e.g. Rv3003c (ilvB), etc. Contains PS00187 Thiamine pyrophosphate enzymes signature. P9WG39 No intermediary metabolism and respiration P66946 P9WG39 GO:0009082,GO:0000287,GO:0030976,GO:0003984 2.2.1.6 P9WG39 NC_000962.3 Mycobrowser_v5 CDS 2096183 2096599 . - 0 Rv1846c blaI Involved in transcriptional mechanism Transcriptional repressor BlaI Rv1846c, (MTCY359.27), len: 138 aa. BlaI, transcriptional repressor. Equivalent to MLCB1788.17|AL008609 hypothetical protein from Mycobacterium leprae (142 aa), FASTA scores: opt: 736 E(): 0, (95.1% identity in 123 aa overlap). Also similar to BLAI_BACLI|P06555 penicillinase repressor (128 aa), fasta scores: opt: 114, E(): 0.12, (23.7% identity in 131 aa overlap). P9WMJ5 No regulatory proteins 2G9W P95163 P9WMJ5 GO:0005737,GO:0016481,GO:0046677,GO:0016566,GO:0006350,GO:0003677 P9WMJ5 NC_000962.3 Mycobrowser_v5 CDS 2099694 2100329 . + 0 Rv1851 ureF Probably facilitates nickel incorporation Urease accessory protein UreF Rv1851, (MTCY359.22c), len: 211 aa. UreF, urease accessory protein. Identical to UREF_MYCTU|P50050 from M. tuberculosis. P9WFE5 No intermediary metabolism and respiration P50050 P9WFE5 GO:0016151,GO:0006807,GO:0005737 P9WFE5 NC_000962.3 Mycobrowser_v5 CDS 2069080 2069709 . + 0 Rv1822 pgsA2 Thought to be involved in cardiolipin biosynthesis; generates cardiolipin from phosphatidylglycerol and CDP-diacylglycerol [catalytic activity: may be: phosphatidylglycerol + phosphatidylglycerol -> cardiolipin + glycerol, or: CDP-diacylglycerol + glycerol 3-phosphate = CMP + 3-(3-phosphatidyl)-glycerol 1-phosphate]. Probable CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase PgsA2 (PGP synthase) (phosphatidylglycerophosphate synthase) (3-phosphatidyl-1'-glycerol-3'phosphate synthase) Rv1822, (MTCY1A11.21c), len: 209 aa. Probable pgsA2, CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyl-transferase (see citation below), integral membrane protein, equivalent to AL008609|MLCB1788_17 phosphatidyltransferase from Mycobacterium leprae (206 aa), FASTA score: (76.6% identity in 205 aa overlap). Also highly similar or similar to others e.g. CAB88885.1|AL353861 putative CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyl-transferase from Streptomyces coelicolor (215 aa); AAC44003.1|U29587 phosphatidylglycerol phosphate synthase from Rhodobacter sphaeroides (227 aa); NP_405431.1|NC_003143 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase from Yersinia pestis (182 aa); P06978|PGSA_ECOLI CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase from Escherichia coli (181 aa), FASTA scores: opt: 252, E(): 2.8e-09, (29.7% identity in 175 aa overlap); etc. Also similar to Rv2746c|PGSA3|MTV002.11c CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (PGP synthase) from Mycobacterium tuberculosis (209 aa). Contains PS00379 CDP-alcohol phosphatidyltransferases signature; and PS00075 Dihydrofolate reductase signature. Belongs to the CDP-alcohol phosphatidyltransferase class-I family. P9WPG5 No lipid metabolism P63753 P9WPG5 GO:0016021,GO:0008654,GO:0005886,GO:0008444 2.7.8.5 P9WPG5 NC_000962.3 Mycobrowser_v5 CDS 2069702 2070625 . + 0 Rv1823 Rv1823 Function unknown Conserved protein Rv1823, (MTCY01A11.20), len: 307 aa. Conserved protein, similar to P71582|MTCY10H4.12|RV0012 hypothetical protein CY10H4.12 from Mycobacterium tuberculosis (262 aa), FASTA scores: opt: 304, E(): 1.5e-12, (30.1% identity in 246 aa overlap). P9WFG1 No conserved hypotheticals P64891 P9WFG1 GO:0005886,GO:0016021 P9WFG1 NC_000962.3 Mycobrowser_v5 CDS 2070654 2071019 . + 0 Rv1824 Rv1824 Unknown Conserved hypothetical membrane protein Rv1824, (MTCY1A11.19c), len: 121 aa. Conserved hypothetical membrane protein similar to P28265|SBP_BACSU sbp protein from Bacillus subtilis (121 aa), FASTA scores: opt: 261, E(): 1.9e-12, (38.9% identity in 113 aa overlap). P9WLR7 No cell wall and cell processes P64893 P9WLR7 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2071036 2071914 . + 0 Rv1825 Rv1825 Function unknown Conserved protein Rv1825, (MTCY1A11.18c), len: 292 aa. Conserved protein, weak similarity to Mycobacterium tuberculosis hypothetical proteins Q50610|MTCY1A11.20C|Rv1823|Z78020 (307 aa), FASTA scores: opt: 182, E(): 0.00044, (29.9% identity in 204 aa overlap); and Rv0012. Has a hydrophobic stretch, TMhelix from aa 67 to 85. P9WFG3 No conserved hypotheticals 3GMG P64895 P9WFG3 GO:0005886,GO:0016021 P9WFG3 NC_000962.3 Mycobrowser_v5 CDS 2074841 2075518 . + 0 Rv1830 Rv1830 Function unknown Conserved hypothetical protein Rv1830, (MTCY1A11.13c), len: 225 aa. Conserved hypothetical protein, equivalent to Mycobacterium leprae hypothetical protein MLCB1788.33c|AL008609|O32916 (231 aa), FASTA scores: opt: 1307, E(): 0, (89.6% identity in 231 aa overlap). P9WME5 No conserved hypotheticals P9WME5 GO:0006355,GO:0006350,GO:0003700,GO:0000166 P9WME5 NC_000962.3 Mycobrowser_v5 CDS 2157382 2157987 . - 0 Rv1911c lppC Unknown Probable lipoprotein LppC Rv1911c, (MTCY180.07), len: 201 aa. Probable lipoprotein lppC, contains appropriately positioned prokaryotic membrane lipoprotein lipid attachment site (PS00013). Very similar to downstream ORF MTCY180.08 (204 aa) (although this lacks lipoprotein motif), FASTA score: opt: 831, E(): 0, (64.0% identity in 200 aa overlap). Also similar to Q9Z729|Y877_CHLPN hypothetical protein CPN0877 from Chlamydia pneumoniae (strain CWL029) (150 aa). P9WFN3 No cell wall and cell processes P67224 P9WFN3 P9WFN3 NC_000962.3 Mycobrowser_v5 CDS 2546883 2547752 . + 0 Rv2275 Rv2275 Unknown Conserved hypothetical protein Rv2275, (MTCY339.35c), len: 289 aa. Conserved hypothetical protein. Some similarity to Bacillus subtilis sp|O34351|O34351 YVMC (248 aa), FASTA score: opt: 280, E(): 2.7e -11; 28.2% identity in 227 aa overlap P9WPF9 No conserved hypotheticals 2X9Q P9WPF9 P9WPF9 NC_000962.3 Mycobrowser_v5 CDS 2072596 2073084 . + 0 Rv1827 garA Function unknown Conserved protein with FHA domain, GarA Rv1827, (MTCY1A11.16c), len: 162 aa. GarA, conserved protein with forkhead-associated domain at C-terminus (see citation below), equivalent to O32919|MLCB1788.36c hypothetical protein from Mycobacterium leprae (162 aa), FASTA scores: opt: 888, E(): 0, (87.0% identity in 161 aa overlap). Putative physiological substrate of PknB and PknG. P9WJA9 No conserved hypotheticals 2KFU P64897 P9WJA9 P9WJA9 NC_000962.3 Mycobrowser_v5 CDS 2073081 2073824 . + 0 Rv1828 Rv1828 Function unknown Conserved protein Rv1828, (MTCY1A11.15c), len: 247 aa. Conserved protein, equivalent to O32918|MLCB1788.35c|AL008609 hypothetical protein from Mycobacterium leprae (251 aa), FASTA scores: opt: 1397, E(): 0, (87.6% identity in 251 aa overlap). P9WME7 No conserved hypotheticals P67669 P9WME7 GO:0006355,GO:0006350,GO:0003700,GO:0000166 P9WME7 NC_000962.3 Mycobrowser_v5 CDS 2976989 2977234 . - 0 Rv2654c Rv2654c Unknown Possible PhiRv2 prophage protein Rv2654c, (MTCY441.23c), len: 81 aa. Hypothetical ala-rich protein, possibly phiRv2 phage protein (see citation below), similar to C-terminus of Q9HNI3|VNG2091H hypothetical protein from Halobacterium sp. strain NRC-1 (212 aa), FASTA scores: opt: 122, E(): 0.46, (43.05% identity in 79 aa overlap). P9WJ11 No insertion seqs and phages P9WJ11 NC_000962.3 Mycobrowser_v5 CDS 2078929 2079789 . - 0 Rv1833c Rv1833c May act on a wide range of 1-haloalkanes, haloalcohols, haloalkenes and some haloaromatic compounds [catalytic activity: 1-haloalkane + H(2)O <=> a primary alcohol + halide] Possible haloalkane dehalogenase Rv1833c, (MTCY1A11.10), len: 286 aa. Possible haloalkane dehalogenase. Similar to several haloalkane dehalogenase e.g. CAB45532.1|AJ243259 from Mycobacterium bovis (300 aa); also similar to LINB_PSEPA|P51698 1,3,4,6-tetrachloro-1,4-cyclohexadien from Pseudomonas paucimobilis (295 aa), FASTA scores: opt: 314, E(): 1.5e-13, (33.1% identity in 281 aa overlap). P9WMS1 No intermediary metabolism and respiration P64303 P9WMS1 GO:0018786 3.8.1.5 P9WMS1 NC_000962.3 Mycobrowser_v5 CDS 2079830 2080696 . + 0 Rv1834 lipZ Unknown Probable hydrolase Rv1834, (MTCY1A11.09c), len: 288 aa. Probable lipZ, hydrolase, some similarity to haloalkane dehalogenases and D16262 hypothetical 38.9 kDa protein (335 aa), FASTA scores: opt: 507, E(): 7.6e-28, (33.0% identity in 300 aa overlap). P9WLR1 No intermediary metabolism and respiration Q50599 P9WLR1 GO:0003824 P9WLR1 NC_000962.3 Mycobrowser_v5 CDS 2080701 2082587 . - 0 Rv1835c Rv1835c Function unknown Conserved hypothetical protein Rv1835c, (MTCY1A11.08), len: 628 aa. Conserved hypothetical protein, some similarity to putative acylases e.g. G216374 glutaryl 7-aca acylase precursor (634 aa) FASTA scores, opt: 202, E(): 3.5e-06, (25.1% identity in 669 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins Rv2800 and Rv1215c. P9WIQ9 No conserved hypotheticals P0A5F5 P9WIQ9 GO:0008152,GO:0008239 P9WIQ9 NC_000962.3 Mycobrowser_v5 CDS 2134273 2134872 . - 0 Rv1885c Rv1885c Involved in the shikimate pathway. Converts chorismate to prephenate in the biosynthesis of tyrosine and phenylalanine. Chorismate mutase Rv1885c, (MTCY180.33), len: 199 aa. Chorismate mutase, AroQ class (See Prakash et al., 2005, Sasso et al., 2005), some similarity to P42517|CHMU_ERWHE monofunctional chorismate mutase (181 aa), FASTA score: opt: 181, E(): 0.00017, (28.6% identity in 133 aa overlap). Contains N-terminal signal sequence. P9WIB9 No intermediary metabolism and respiration 2AO2,2F6L,2FP1,2FP2 O07746 P9WIB9 GO:0046417 P9WIB9 NC_000962.3 Mycobrowser_v5 CDS 2084756 2086981 . - 0 Rv1837c glcB Involved in glyoxylate bypass (second step), an alternative to the tricarboxylic acid cycle [catalytic activity: L-malate + CoA = acetyl-CoA + H(2)O + glyoxylate] Malate synthase G GlcB Rv1837c, (MTCY1A11.06), len: 741 aa. glcB, malate synthase G (see citations below), highly similar to MASY_CORGL|P42450 malate synthase (738 aa), FASTA score: opt: 2961, E(): 0, (61.3% identity in 724 aa overlap). Belongs to the malate synthase G family. P9WK17 No intermediary metabolism and respiration 1N8I,1N8W,2GQ3 P0A5J4 P9WK17 GO:0006097,GO:0004474,GO:0006099,GO:0005737 2.3.3.9 P9WK17 NC_000962.3 Mycobrowser_v5 CDS 2087257 2087652 . - 0 Rv1838c vapC13 Unknown Possible toxin VapC13 Rv1838c, (MTCY359.35), len: 131 aa. Possible vapC13, toxin, part of toxin-antitoxin (TA) operon with Rv1839c, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Part of 14-membered Mycobacterium tuberculosis protein family with Rv2863|MTV003.09|AL008883 (126 aa), FASTA scores: opt: 293, E(): 1.5e-14, (38.2% identity in 123 aa overlap); Rv0749, Rv0277c, Rv2530c, etc. Also similar to AJ248288|CNSPAX06_181 Pyrococcus abyssi complete genome (136 aa), FASTA scores: opt: 197, E(): 2.2e-07, (33. 1% identity in 133 aa overlap). P9WFA1 No virulence, detoxification, adaptation P64901 P9WFA1 P9WFA1 NC_000962.3 Mycobrowser_v5 CDS 2087649 2087912 . - 0 Rv1839c vapB13 Unknown Possible antitoxin VapB13 Rv1839c, (MTCY359.34), len: 87 aa. Possible vapB13, antitoxin, part of toxin-antitoxin (TA) operon with Rv1838c (See Arcus et al., 2005; Pandey and Gerdes, 2005). Some similarity to others in M. tuberculosis e.g. Rv0239, Rv0662c P9WJ51 No virulence, detoxification, adaptation P9WJ51 GO:0003677 NC_000962.3 Mycobrowser_v5 CDS 2100340 2101014 . + 0 Rv1852 ureG Probably facilitates nickel incorporation Urease accessory protein UreG Rv1852, (MTCY359.21c), len: 224 aa. UreG, urease accessory protein. Identical to UREG_MYCTU|P50051 from M. tuberculosis. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the UreG family. P9WFE3 No intermediary metabolism and respiration P0A664 P9WFE3 GO:0005525,GO:0005737,GO:0016151,GO:0005524 P9WFE3 NC_000962.3 Mycobrowser_v5 CDS 2110591 2111361 . - 0 Rv1863c Rv1863c Unknown Probable conserved integral membrane protein Rv1863c, (MTCY359.10), len: 256 aa. Probable conserved integral membrane protein, similar to Rv0804|Z95618|MTCY7H7A.05 Hypothetical protein from Mycobacterium tuberculosis (209 aa), FASTA scores: opt: 199, E(): 1e-06, (33.2% identity in 220 aa overlap); and Rv0658c. P95152 No cell wall and cell processes P95152 P95152 GO:0016020 P95152 NC_000962.3 Mycobrowser_v5 CDS 2225413 2225832 . - 0 Rv1982c vapC36 Unknown Possible toxin VapC36. Contains PIN domain. Rv1982c, (MTCY39.37), len: 139 aa. Possible vapC36, toxin, part of toxin-antitoxin (TA) operon with Rv1982A, contains PIN domain, see Arcus et al. 2005. belongs to the UPF0110 family. Similar to Rv0624|Z92772|MTY20H10.05 from Mycobacterium tuberculosis (131 aa), FASTA scores: opt: 288, E(): 4.1e-14, (40.2% identity in 127 aa overlap); also similar to Rv0624, Rv2759c, and Rv0609 P9WF65 No virulence, detoxification, adaptation P0A652 P9WF65 P9WF65 NC_000962.3 Mycobrowser_v5 CDS 2089681 2090718 . - 0 Rv1841c Rv1841c Unknown Conserved hypothetical membrane protein Rv1841c, (MTCY359.32), len: 345 aa. Conserved hypothetical membrane protein. Some similarity to O07585|YHDP_BACSU hypothetical 49.9 kDa protein from Bacillus subtilis (444 aa), FASTA scores: opt: 620, E(): 0, (31.1% identity in 350 aa overlap). Also similar to other Mycobacterium tuberculosis proteins e.g. Rv1842c, Rv2366c. P9WLQ7 No cell wall and cell processes Q50593 P9WLQ7 GO:0005886,GO:0016021 P9WLQ7 NC_000962.3 Mycobrowser_v5 CDS 2092259 2093698 . - 0 Rv1843c guaB1 Involved in GMP biosynthesis [catalytic activity: inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH] Probable inosine-5'-monophosphate dehydrogenase GuaB1(imp dehydrogenase) (IMPDH) (IMPD) Rv1843c, (MTCY359.30), len: 479 aa. Probable guaB1, inosine-5'-monophosphate dehydrogenase. Similar to others e.g. IMDH_BACSU|P21879 from Bacillus subtilis (513 aa), FASTA score: opt: 904, E(): 0, (37.8% identity in 471 aa overlap). Similar to other Mycobacterium tuberculosis proteins e.g. guaB2, Rv3411c. P9WKI3 No intermediary metabolism and respiration P65172 P9WKI3 GO:0055114,GO:0003938 1.-.-.- P9WKI3 NC_000962.3 Mycobrowser_v5 CDS 2095218 2096168 . - 0 Rv1845c blaR Involved in transcriptional mechanism Possible sensor-transducer protein BlaR Rv1845c, (MTCY359.28), len: 316 aa. Possible blaR, sensor-transducer protein. Conserved hypothetical transmembrane protein. Equivalent to MLCB1788.18|AL008609 Hypothetical protein from Mycobacterium leprae (316 aa), FASTA scores: opt: 1762, E(): 0, (87.6% identity in 314 aa overlap). Similar to proteins in Streptomyces coelicolor e.g. SC10A7.04|AL078618.1. P95164 No cell wall and cell processes P95164 P95164 GO:0046872,GO:0004222,GO:0006508,GO:0016021 P95164 NC_000962.3 Mycobrowser_v5 CDS 2096877 2097299 . + 0 Rv1847 Rv1847 Function unknown Conserved protein Rv1847, (MTCY359.26c), len: 140 aa. Conserved protein, possible thioesterase, some similarity to YBDB proteins of Escherichia coli and H. influenzae e.g. P15050|YBDB_ECOLI hypothetical 15.0 KD protein in ENTA-CSTA intergenic region (137 aa), FASTA scores: opt: 232, E(): 6.6e-10, (35.8% identity in 106 aa overlap); C48956|G142208 thioesterase from Arthrobacter sp (151 aa), FASTA score: opt: 254, E(): 1.7e-11, (33.3% identity in 138 aa overlap). Also similar to AF064959|AF064959_1 hypothetical protein from Coxiella burnetii (148 aa), FASTA score: opt: 264, E(): 9.3e- 12, (36.8% identity in 117 aa overlap). P9WIM3 No conserved hypotheticals 3S4K P95162 P9WIM3 GO:0016787 3.1.2.- P9WIM3 NC_000962.3 Mycobrowser_v5 CDS 2097348 2097650 . + 0 Rv1848 ureA Involved in the conversion of urea to NH3 [catalytic activity: urea + H2O = CO2 + 2 NH3] Urease gamma subunit UreA (urea amidohydrolase) Rv1848, (MTCY359.25c), len: 100 aa. UreA, urease gamma subunit. Similar to URE3_MYCTU|P50043 from Mycobacterium tuberculosis (100 aa), FASTA scores: opt: 630, E(): 1.3e-36, (99.0% identity in 100 aa overlap). Belongs to the urease gamma subunit family. P9WFE7 No intermediary metabolism and respiration 2FVH P0A676 P9WFE7 GO:0016151,GO:0019627,GO:0009039,GO:0005737 3.5.1.5 P9WFE7 NC_000962.3 Mycobrowser_v5 CDS 2097647 2097961 . + 0 Rv1849 ureB Involved in the conversion of urea to NH3 [catalytic activity: urea + H2O = CO2 + 2 NH3] Urease beta subunit UreB (urea amidohydrolase) Rv1849, (MTCY359.24c), len: 104 aa. UreB, urease beta subunit. Identical to URE2_MYCTU|P50048 urease beta subunit from Mycobacterium tuberculosis (100 aa). Belongs to the urease gamma subunit family. P9WFE9 No intermediary metabolism and respiration P0A662 P9WFE9 GO:0016151,GO:0006807,GO:0009039,GO:0005737 3.5.1.5 P9WFE9 NC_000962.3 Mycobrowser_v5 CDS 2101022 2101648 . + 0 Rv1853 ureD Probably facilitates nickel incorporation Probable urease accessory protein UreD Rv1853, (MTCY359.20c), len: 208 aa. UreD, probable urease accessory protein. Similar to URED_YEREN|P42868 Urease operon ureD protein from Yersinia enterocolitica (325 aa), Fasta scores: opt: 114, E(): 0.37, (25.2% identity in 119 aa overlap). P95161 No intermediary metabolism and respiration P95161 P95161 GO:0006807,GO:0016151 P95161 NC_000962.3 Mycobrowser_v5 CDS 2103184 2104107 . - 0 Rv1855c Rv1855c Function unknown; probably involved in cellular metabolism Possible oxidoreductase Rv1855c, (MTCY359.18), len: 307 aa. Possible oxidoreductase, possibly a monooxygenase. Contains PS00217 Sugar transport proteins signature 2, probably fortuitously. Similar to G487716 (78-11) lincomycin production genes (29.2% identity in 154 aa overlap). Also similar to other Mycobacterium tuberculosis proteins e.g. Rv0953c, Rv0791c, Rv0132c, Rv2951c, etc. P95159 No intermediary metabolism and respiration P95159 P95159 GO:0016491,GO:0055114 1.-.-.- P95159 NC_000962.3 Mycobrowser_v5 CDS 2104146 2104823 . - 0 Rv1856c Rv1856c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv1856c, (MTCY359.17), len: 225 aa. Possible oxidoreductase. Equivalent to MLCB1788.11c|AL008609 oxidoreductase from Mycobacterium leprae (224 aa), FASTA scores: opt: 1211, E(): 0; (80.4% identity in 224 aa overlap). Some similarity to dehydrogenases of short-chain dehydrogenase/reductase family and fatty-acyl CoA reductases e.g. P16543|DHK2_STRVN granaticin polyketide synthase P (249 aa), FASTA score: opt: 194, E(): 1.1e-05, (32.5% identity in 237 aa overlap). P9WGQ1 No intermediary metabolism and respiration P95158 P9WGQ1 GO:0055114,GO:0016491 1.-.-.- P9WGQ1 NC_000962.3 Mycobrowser_v5 CDS 2104985 2105770 . + 0 Rv1857 modA Involved in the active transport of molybdenum into the cell across the membrane (import). Part of the binding-protein-dependent transport system modabc. Probable molybdate-binding lipoprotein ModA Rv1857, (MTCY359.16c), len: 261 aa. Probable modA, molybdate-binding protein attached to membrane by lipid-modified N-terminal cysteine (contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site), component of molybdate transport system (see citations below). Shows strong similarity to precursors of periplasmic molybdate/sulphate binding proteins e.g. O31229|Y10817|ANY108174 ModA from Arthrobacter nicotinovorans (260 aa), FASTA score: opt: 725, E(): 0, (47.8% identity in 249 aa overlap). P9WGU3 No cell wall and cell processes P0A5Y0 P9WGU3 GO:0015412,GO:0030288,GO:0005886,GO:0015689 P9WGU3 NC_000962.3 Mycobrowser_v5 CDS 2109165 2109470 . + 0 Rv1861 Rv1861 Function unknown Probable conserved transmembrane protein Rv1861, (MTCY359.12c), len: 101 aa. Probable conserved transmembrane protein, showing weak similarity to AE002069|AE002069_10 hypothetical protein from Deinococcus radiodurans (146 aa), FASTA scores: opt: 154, E(): 0.0027, (30.8% identity in 104 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P95154 No cell wall and cell processes P95154 P95154 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2111354 2112109 . - 0 Rv1864c Rv1864c Function unknown Conserved protein Rv1864c, (MTCY359.09), len: 251 aa. Conserved protein. Similar to other hypothetical proteins e.g. AL031317|SC6G4.43 from Streptomyces coelicolor cosmid 6G (233 aa), FASTA scores: opt: 716, E(): 0, (54.4% identity in 215 aa overlap); also P43976|YIIM_HAEIN hypothetical protein hi0278 (221 aa), FASTA scores: opt: 223, E(): 3.8e-08, (29.5% identity in 173 aa overlap). P95151 No conserved hypotheticals P95151 P95151 GO:0030151,GO:0030170,GO:0003824 P95151 NC_000962.3 Mycobrowser_v5 CDS 2137519 2138079 . - 0 Rv1888c Rv1888c Unknown Possible transmembrane protein Rv1888c, (MTCY180.30), len: 186 aa. Possible transmembrane protein. O07744 No cell wall and cell processes O07744 GO:0016021 O07744 NC_000962.3 Mycobrowser_v5 CDS 2107736 2108713 . + 0 Rv1860 apa Unknown (could mediate bacterial attachment to host cells). Alanine and proline rich secreted protein Apa (fibronectin attachment protein) (immunogenic protein MPT32) (antigen MPT-32) (45-kDa glycoprotein) (45/47 kDa antigen) Rv1860, (MT1908, MTCY359.0013), len: 325 aa. Apa (alternate gene names: mpt32, modD), Ala-, Pro-rich 45/47 kDa secreted protein, very similar to P46842|N43L_MYCLE from Mycobacterium leprae (287 aa), FASTA scores: opt: 1166, E(): 0, (66.4% identity in 298 aa overlap). Known to be glycosylated fibronectin-binding protein (see some citations). Changes in the mannosylation pattern of this protein affect its ability to stimulate T-lymphocyte response. Major immunodominant antigen that has potential as a vaccine against tuberculosis. APA-ELISA could be used in diagnosis. P9WIR7 No cell wall and cell processes Q50906 P9WIR7 GO:0005576,GO:0050840 P9WIR7 NC_000962.3 Mycobrowser_v5 CDS 2109544 2110584 . + 0 Rv1862 adhA Catalyzes the reversible oxidation of ethanol to acetaldehyde with the concomitant reduction of NAD Probable alcohol dehydrogenase AdhA Rv1862, (MTCY359.11), len: 346 aa. Probable adhA, alcohol dehydrogenase, similar to ADH2_BACST|P42327 alcohol dehydrogenase (339 aa), FASTA scores: opt: 630, E(): 2.4e-32 (34.4% identity in 320 aa overlap). Contains PS00059 Zinc-containing alcohol dehydrogenases signature. P9WQC1 No intermediary metabolism and respiration P95153 P9WQC1 GO:0055114,GO:0008270,GO:0004022 1.1.1.1 P9WQC1 NC_000962.3 Mycobrowser_v5 CDS 2112106 2112966 . - 0 Rv1865c Rv1865c Function unknown; probably involved in cellular metabolism Probable short-chain type dehydrogenase Rv1865c, (MTCY359.08), len: 286 aa. Probable short-chain dehydrogenase, highly similar to C-terminus of NP_301650.1|NC_00267 putative oxidoreductase from Mycobacterium leprae (596 aa). Also similar to various dehydrogenases, generally belonging to short-chain family, e.g. AAG02168.1|AF212041_24|AF212041 3-oxoacyl-(acylcarrier protein) reductase from Zymomonas mobilis (251 aa); P50198|LINX_PSEPA 2,5-dichloro-2,5-cyclohexadiene-1,4-DIOL dehydrogenase from Sphingomonas paucimobilis (250 aa); NP_105680.1|NC_002678 sorbitol dehydrogenase (also similar to acetoin reductase) from Mesorhizobium loti (256 aa); etc. And highly similar to C-terminus of ephD|Rv2214c|MTCY190.25c from Mycobacterium tuberculosis (592 aa); and many other oxidoreductases from Mycobacterium tuberculosis e.g. Y00P_MYCTU|Q10402 putative oxidoreductase (650 aa), FASTA scores: opt: 439, E(): 8.9e-20, (32.5% identity in 280 aa overlap). Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. P95150 No intermediary metabolism and respiration P95150 P95150 GO:0055114,GO:0016491 1.-.-.- P95150 NC_000962.3 Mycobrowser_v5 CDS 2145214 2146245 . - 0 Rv1899c lppD Unknown Possible lipoprotein LppD Rv1899c, (MTCY180.19), len: 343 aa. Possible lipoprotein; contains appropriately localized lipoprotein lipid attachment site (PS00013). Some similarity to C-terminal part of AE000717|AE000717_4 hypothetical protein from Aquifex aeolicus section 49 (165 aa), FASTA results: opt: 372, E(): 2.3e-14, (43.5% identity in 147 aa overlap); and Q44020 4-hydroxybutyrate dehydrogenase (173 aa), FASTA results: opt: 272, E(): 4.7e-09, (35.8% identity in 165 aa overlap). P9WK29 No cell wall and cell processes O07733 P9WK29 P9WK29 NC_000962.3 Mycobrowser_v5 CDS 2579504 2579935 . - 0 Rv2307B Rv2307B Unknown Hypothetical glycine rich protein Rv2307B, len: 143 aa. Hypothetical unknown Gly- rich protein. Equivalent to AAK46653 from Mycobacterium tuberculosis strain CDC1551 (133 aa) but longer 10 aa. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q79FG2 No conserved hypotheticals Q79FG2 NC_000962.3 Mycobrowser_v5 CDS 2117347 2119446 . + 0 Rv1868 Rv1868 Function unknown Conserved hypothetical protein Rv1868, (MTCY359.05c), len: 699 aa. Conserved hypothetical protein, similar to products of three consecutive ORFS in Mycobacterium leprae MLCB2052.18|Z98604|B2052 (257 aa), FASTA scores: opt: 314, E(): 9.9e-12, (35.2% identity in 213 aa overlap); MLCB2052.17, and MLCB2052.16. Also similar to M. tuberculosis hypothetical protein Rv2047c. P95147 No conserved hypotheticals P95147 GO:0003824,GO:0008152 P95147 NC_000962.3 Mycobrowser_v5 CDS 2119460 2120695 . - 0 Rv1869c Rv1869c Function unknown; probably involved in cellular metabolism Probable reductase Rv1869c, (MTCY359.04), len: 411 aa. Probable reductase (1.-.-.-). Similar to several reductases e.g. CAC04223.1|AL391515 putative ferredoxin reductase from Streptomyces coelicolor (420 aa); THCD_RHOSO|P43494 rhodocoxin reductase (426 aa), FASTA scores: opt: 904, E(): 0, (40.8% identity in 370 aa overlap). Also similar to Mycobacterium tuberculosis proteins Rv0688 (406 aa) (39.9% identity in 391 aa overlap); and Rv0253 (nitrite reductase subunit). P95146 No intermediary metabolism and respiration P95146 P95146 GO:0055114,GO:0016491,GO:0050660 1.-.-.- P95146 NC_000962.3 Mycobrowser_v5 CDS 2120795 2121430 . - 0 Rv1870c Rv1870c Unknown Conserved hypothetical protein Rv1870c, (MTCY359.03), len: 211 aa. Conserved hypothetical protein. Some similarity to SC6F7.17c hypothetical protein from Streptomyces coelicolor (216 aa). P95145 No conserved hypotheticals P95145 P95145 NC_000962.3 Mycobrowser_v5 CDS 2121495 2121884 . - 0 Rv1871c Rv1871c Function unknown Conserved protein Rv1871c, (MTCY359.02), len: 129 aa. Conserved protein, similar to Mycobacterium tuberculosis hypothetical proteins Q11057|Rv1261|MTCY50.21 (149 aa), FASTA score: opt: 125, E(): 0.019, (32.6% identity in 89 aa overlap); Rv0523c, and Rv1598c. P95144 No conserved hypotheticals P95144 P95144 P95144 NC_000962.3 Mycobrowser_v5 CDS 2124381 2124824 . + 0 Rv1875 Rv1875 Function unknown Conserved protein Rv1875, (MTCY180.43c), len: 147 aa. Conserved protein. Some similarity to Mycobacterium tuberculosis hypothetical proteins e.g. Rv1155|MTCI65.22|Z95584 (147 aa), FASTA scores: opt: 178, E(): 7.4e-06, (26.9% identity in 130 aa overlap); Rv0121c and Rv2074. Also similar to AL079356|SC6G9.21 hypothetical protein from Streptomyces coelicolor (144 aa), FASTA scores: opt: 239, E(): 3.1 e-09, (38.7% identity in 137 aa overlap). O07754 No conserved hypotheticals O07754 O07754 GO:0010181 O07754 NC_000962.3 Mycobrowser_v5 CDS 2194970 2195347 . - 0 Rv1943c mazE5 Function unknown Possible antitoxin MazE5 Rv1943c, (MTCY09F9.21), len: 125 aa. Possible mazE5, antitoxin, part of toxin-antitoxin (TA) operon with Rv1942c (See Pandey and Gerdes, 2005; Zhu et al., 2006), shows some similarity with Rv1946c|MTCY09F9.18|lppG possible conserved lipoprotein from Mycobacterium tuberculosis (150 aa), FASTA score: (71.4% identity in 28 aa overlap). P9WJ89 No virulence, detoxification, adaptation P9WJ89 NC_000962.3 Mycobrowser_v5 CDS 2138444 2138617 . - 0 Rv1888A Rv1888A Function unknown Conserved hypothetical protein Rv1888A, len: 57 aa. Conserved hypothetical protein. Possibly continuation of Rv1889c, part of large family of Mycobacterium tuberculosis proteins with conserved N-terminal domain of ~ 120 aa. Includes: C-terminus of Rv0726c|P95074 conserved hypothetical protein (367 aa), FASTA scores: opt: 295, E(): 3.1e-15, (73.684% identity in 57 aa overlap); C-terminus of Rv3399|Q50726|MTCY78.29c conserved hypothetical protein (348 aa), FASTA scores: opt: 504, E(): 7.3e-29, (64.2% identity in 120 aa overlap); C-terminus of Rv0731c; etc. Q79FJ0 No conserved hypotheticals Q79FJ0 Q79FJ0 GO:0008168 Q79FJ0 NC_000962.3 Mycobrowser_v5 CDS 3501334 3501732 . + 0 Rv3135 PPE50 Function unknown PPE family protein PPE50 Rv3135, (MTCY03A2.23c), len: 132 aa. PPE50, Member of the Mycobacterium tuberculosis Ala-, Gly-rich PPE family, similar to P95190|Rv3136|MTCY03A2.22c (380 aa), FASTA scores: opt: 494, E(): 6.7e-25, (57.25% identity in 131 aa overlap) (next ORF downstream), MTY21C12_9, MTCY3C7_24, MTCI125_27, MTV049_12, MTV049_9, MTV049_11, MTCY274_24 etc. Q6MX07 No PE/PPE Q6MX07 Q6MX07 Q6MX07 NC_000962.3 Mycobrowser_v5 CDS 2128022 2129374 . + 0 Rv1878 glnA3 Involved in glutamine biosynthesis [catalytic activity: ATP + L-glutamate + NH(3) = ADP + glutamine + orthophosphate]. Probable glutamine synthetase GlnA3 (glutamine synthase) (GS-I) Rv1878, (MTCY180.40c), len: 450 aa. Probable glnA3, glutamine synthetase class I, similar to many e.g. GLNA_BACCE|P19064 from Bacillus cereus (443 aa), FASTA results: opt: 497, E(): 5.2e-23, (29.0% identity in 331 aa overlap); etc. Also similar to C-terminus of FLUG_EMENI|P38094 flug protein from emericella nidulans (865 aa), FASTA scores: opt: 227, E (): 6.4e-13, (29.9% identity in 394 aa overlap). Note that the downstream ORF MTCY180.39c is similar to the N-terminus. Also similar to three other potential glutamine synthases in M. tuberculosis: Q10378|GLN2_MYCTU|GLNA2|Rv2222c|MT2280|MTCY190.33c|MTCY427.03c; Rv2860c|MTV003.06c|glnA4 and Rv2220|glnA1. Belongs to the glutamine synthetase family. O07752 No intermediary metabolism and respiration O07752 O07752 GO:0006807,GO:0004356 6.3.1.2 O07752 NC_000962.3 Mycobrowser_v5 CDS 2285628 2286530 . - 0 Rv2040c Rv2040c Thought to be involved in active transport of sugar across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable sugar-transport integral membrane protein ABC transporter Rv2040c, (MTV018.27c), len: 300 aa. Probable sugar-transport integral membrane protein ABC transporter (see citation below), similar to many. O53484 No cell wall and cell processes O53484 O53484 GO:0005886,GO:0006810,GO:0005215,GO:0016021 O53484 NC_000962.3 Mycobrowser_v5 CDS 2326944 2327501 . + 0 Rv2069 sigC Involved in promoter recognition, transcription initiation. RNA polymerase sigma factor, ECF subfamily, SigC Rv2069, (MTCY49.08), len: 185 aa. SigC, RNA polymerase sigma factor, ECF subfamily (see Gomez et al., 1997; Chen et al., 2000), similar to many. P9WGH1 No information pathways 2O7G,2O8X P66809 P9WGH1 GO:0016987,GO:0003700,GO:0006352,GO:0006355 P9WGH1 NC_000962.3 Mycobrowser_v5 CDS 2580028 2580210 . - 0 Rv2307D Rv2307D Unknown Hypothetical protein Rv2307D, len: 60 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L7N683 No conserved hypotheticals L7N683 L7N683 NC_000962.3 Mycobrowser_v5 CDS 2131907 2132329 . - 0 Rv1881c lppE Unknown Possible conserved lipoprotein LppE Rv1881c, (MTCY180.37), len: 140 aa. Possible lppE, lipoprotein, showing some similarity to L12238|MSG18S19K_1 19K antigen from Mycobacterium intracellulare (162 aa), FASTA scores: opt: 137, E(): 0.0069, (27.6% identity in 156 aa overlap). Contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O07750 No cell wall and cell processes O07750 O07750 NC_000962.3 Mycobrowser_v5 CDS 2133231 2133692 . - 0 Rv1883c Rv1883c Function unknown Conserved hypothetical protein Rv1883c, (MTCY180.35), len: 153 aa. Conserved hypothetical protein, some similarity to hypothetical proteins e.g. Rv2778c|AL008967|MTV002.43 from Mycobacterium tuberculosis (156 aa), FASTA score: opt: 212, E(): 3.1e-08, (34.4% identity in 151 aa overlap). Also similar to U75434|SAU75434_3 Nsh-OrfB from Streptomyces actuosus (173 aa), FASTA score: opt: 207, E(): 1.8e-07, (40.2% identity in 102 aa overlap). O07748 No conserved hypotheticals O07748 O07748 O07748 NC_000962.3 Mycobrowser_v5 CDS 2133731 2134261 . - 0 Rv1884c rpfC Thought to promote the resuscitation and growth of dormant, nongrowing cell. Could also stimulates the growth of several other high G+C gram+ organisms, e.g. Mycobacterium avium, Mycobacterium bovis (BCG), Mycobacterium kansasii, Mycobacterium smegmatis. Probable resuscitation-promoting factor RpfC Rv1884c, (MTCY180.34), len: 176 aa. Probable rpfC, resuscitation promoting factor (see citation below), similar to Z96935|MLRPF_1 resusicitation-promoting factor from Micrococcus luteus (220 aa), FASTA score: opt: 287, E() : 3.3e-11, (40.0% identity in 120 aa overlap). Also similar to others from Mycobacterium tuberculosis: Rv2389c|MTCY253.32|RPFD probable resuscitation-promoting factor (154 aa), FASTA score: opt: 382, E(): 7.1e-17, (55.4% identity in 101 aa overlap); Rv0867c|RPFA (N-terminal part), Rv2450c|RPFE, and Rv1009|RPFB (C-terminal part). Predicted possible vaccine candidate (See Zvi et al., 2008). O07747 No cell wall and cell processes O07747 O07747 GO:0005576 O07747 NC_000962.3 Mycobrowser_v5 CDS 2138661 2139017 . - 0 Rv1889c Rv1889c Function unknown Conserved hypothetical protein Rv1889c, (MTCY180.29), len: 118 aa. Conserved hypothetical protein. Part of large family of Mycobacterium tuberculosis proteins with conserved N-terminal domain of ~120 aa. Includes: Rv3399|Q50726|MTCY78.29C conserved hypothetical protein (348 aa), FASTA results: opt: 504, E(): 7.3e-29, (64.2% identity in 120 aa overlap); Rv0726c|P95074; Rv0731c; etc. Rv1888A possibly continuation of this CDS. O07743 No conserved hypotheticals O07743 O07743 GO:0008168 O07743 NC_000962.3 Mycobrowser_v5 CDS 2225841 2226101 . - 0 Rv1982A vapB36 Unknown Possible antitoxin VapB36 Rv1982A, len: 86 aa. Possible vapB36, antitoxin, part of toxin-antitoxin (TA) operon with Rv1982c, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. Rv0623, Rv2760c, Rv0608 P9WJ29 No virulence, detoxification, adaptation P9WJ29 NC_000962.3 Mycobrowser_v5 CDS 2316681 2317085 . - 0 Rv2061c Rv2061c Unknown Conserved protein Rv2061c, (MTV019.02c), len: 134 aa. Conserved protein. Similar to many. Contains IPR019965 F420-dependent enzyme, PPOX class, family Rv2061, domain. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O86340 No conserved hypotheticals O86340 O86340 GO:0010181 O86340 NC_000962.3 Mycobrowser_v5 CDS 2510351 2510587 . - 0 Rv2237A Rv2237A Unknown Conserved protein Rv2237A, len: 78 aa. Conserved protein. I6XDU8 No conserved hypotheticals I6XDU8 I6XDU8 NC_000962.3 Mycobrowser_v5 CDS 2136258 2137400 . + 0 Rv1887 Rv1887 Unknown Hypothetical protein Rv1887, (MTCY180.31), len: 380 aa. Hypothetical unknown protein; contains eukaryotic thiol (cysteine) proteases histidine active site at N-terminus (PS00639) and Pro-rich region near C-terminus. O07745 No conserved hypotheticals O07745 O07745 NC_000962.3 Mycobrowser_v5 CDS 2142521 2143675 . + 0 Rv1895 Rv1895 Function unknown; probably involved in cellular metabolism Possible dehydrogenase Rv1895, (MTCY180.23c), len: 384 aa. Possible dehydrogenase, similar to various sorbitol and alcohol dehydrogenases, and to putative glutathione-dependent aldehyde dehydrogenase e.g DHSO_BACSU|Q06004 Sorbitol dehydrogenase from Streptomyces coelicolor (352 aa), FASTA results: opt: 506, E(): 7.2e-24, (30.6% identity in 350 aa overlap); and AL109962|SCJ1.28 putative zinc-containing dehydrogenase from Streptomyces coelicolor (356 aa), FASTA results: opt: 634, E(): 2.9e-30, (34.7% identity in 357 aa overlap). Also similar to other Mycobacterium tuberculosis dehydrogenases. Note that there is a substantial (134 bp) overlap at the C-terminus with the C-terminus of the downstream ORF, although both appear to be true coding regions. O07737 No intermediary metabolism and respiration O07737 O07737 GO:0016491,GO:0008270,GO:0055114 1.1.-.- O07737 NC_000962.3 Mycobrowser_v5 CDS 2143535 2144446 . - 0 Rv1896c Rv1896c Function unknown Conserved hypothetical protein Rv1896c, (MTCY180.22), len: 303 aa. Conserved hypothetical protein. Similar to several (14) hypothetical Mycobacterium tuberculosis proteins e.g. Rv0145|MTCI5.19 (317 aa), FASTA results: opt: 720, E(): 0, (41.6% identity in 308 aa overlap); Q10552|YZ21_MYCTU (325 aa), opt: 689, E(): 0, (40.5% identity in 304 aa overlap); Rv0726c, Rv0731c, Rv3399, etc. and to related proteins in other actinomycetes. Note that there is a substantial (134 bp) overlap at the C-terminus with the C-terminus of the downstream ORF, although both appear to be true coding regions. P9WFH7 No conserved hypotheticals O07736 P9WFH7 GO:0008168 2.1.1.- P9WFH7 NC_000962.3 Mycobrowser_v5 CDS 2144451 2144882 . - 0 Rv1897c Rv1897c Function unknown Conserved hypothetical protein Rv1897c, (MTCY180.21), len: 143 aa. Conserved hypothetical protein. Some similarity to D63706|Q54235 ORF2 from Streptomyces griseus (149 aa), FASTA results: opt: 509, E(): 1.2e-28, (57.3% identity in 150 aa overlap); and Q45303 ORF1 protein from Corynebacterium glutamicum (144 aa), FASTA results: opt: 460, E(): 5.5e-23, (49.7% identity in 143 aa overlap). P9WNS9 No conserved hypotheticals P63995 P9WNS9 GO:0005737,GO:0016788,GO:0019478 3.1.-.- P9WNS9 NC_000962.3 Mycobrowser_v5 CDS 2144940 2145248 . + 0 Rv1898 Rv1898 Function unknown Conserved hypothetical protein Rv1898, (MTCY180.20c), len: 102 aa. Conserved hypothetical protein, some similarity to other hypothetical proteins e.g. Q58452 from methanococcus jannasch II (100 aa), FASTA results: opt: 152, E(): 9.1e-05, (31.5% identity in 92 aa overlap); and AE000771|AE000771_2 from Aquifex aeolicus (157 aa), FASTA results: opt: 246, E(): 3.2e-11, (39.0% identity in 100 aa overlap). P9WFQ1 No conserved hypotheticals P67119 P9WFQ1 P9WFQ1 NC_000962.3 Mycobrowser_v5 CDS 2171061 2171525 . - 0 Rv1919c Rv1919c Function unknown Conserved protein Rv1919c, (MTV050.03c), len: 154 aa. Conserved protein, shows weak similarity to several major pollen antigens e.g. Z72431|BVGC25_1 major allergen bet V 1 from Betula verrucosa (160 aa), FASTA scores: opt: 133, E(): 0.012, (26.8% identity in 149 aa overlap). Also shows some similarity to Rv2574|MTCY227.27C Hypothetical protein from Mycobacterium tuberculosis (167 aa), (27.4% identity in 124 aa overlap). O53961 No conserved hypotheticals O53961 O53961 O53961 NC_000962.3 Mycobrowser_v5 CDS 2147662 2148954 . + 0 Rv1901 cinA Unknown Probable CinA-like protein CinA Rv1901, (MTCY180.17c), len: 430 aa. Probable cinA-like protein, strong similarity to competence damage proteins CinA of Bacillus subtilis and S. pneumoniae. FASTA results: Q55760 hypothetical 44.7 kDa protein (416 aa) opt: 755, E(): 0, (36.0% identity in 433 aa overlap). P9WPE3 No virulence, detoxification, adaptation P63775 P9WPE3 GO:0006777 P9WPE3 NC_000962.3 Mycobrowser_v5 CDS 2149006 2150274 . - 0 Rv1902c nanT Involved in transport of sialic acid across the membrane. Responsible for the translocation of the substrate across the membrane. Probable sialic acid-transport integral membrane protein NanT Rv1902c, (MTCY180.16), len: 422 aa. Probable nanT, sialic acid-transport integral membrane protein, possibly member of major facilitator superfamily (MFS), similar to others e.g. Q48076 sialic acid transporter (407 aa), FASTA results: opt: 443, E(): 5.4e-22, (26.7% identity in 389 aa overlap); etc. Some similarity to MTCI364.12|O05301 conserved hypothetical protein from Mycobacterium tuberculosis (425 aa), FASTA results: opt: 251, E(): 1.1e-09, (23.5% identity in 417 aa overlap). Contains sugar transport proteins signature 2 (PS00217). O07730 No cell wall and cell processes O07730 O07730 GO:0016021,GO:0055085,GO:0015144 NC_000962.3 Mycobrowser_v5 CDS 2150364 2150768 . + 0 Rv1903 Rv1903 Unknown Probable conserved membrane protein Rv1903, (MTCY180.15c), len: 134 aa. Probable conserved membrane protein, similar to Q53868|YPT3_STRCO hypothetical 15.9 kDa protein from Streptomyces coelicolor (148 aa) opt: 323, E(): 1.3e-16, (42.9% identity in 126 aa overlap); and equivalent to AJ000521|MLCOSL672_3 from Mycobacterium leprae (139 aa), FASTA results: opt: 680, E(): 0, (80.6% identity in 129 aa overlap). O07729 No cell wall and cell processes O07729 O07729 NC_000962.3 Mycobrowser_v5 CDS 2150954 2151385 . + 0 Rv1904 Rv1904 Function unknown Conserved hypothetical protein Rv1904, (MTCY180.14c), len: 143 aa. Conserved hypothetical protein, some similarity to other hypothetical Mycobacterium tuberculosis proteins e.g. Rv2638|MTCY441.08|P71937 (148 aa), FASTA results: opt: 456, E(): 2.7e-23, (52.8% identity in 125 aa overlap); Rv1365|Q11035 (128 aa), FASTA results: opt: 393, E(): 1.4e-19, (48.8% identity in 123 aa overlap); and Rv3687c. Also weak similarity to Q9WVX8|RSBV_STRCO anti-sigma B factor antagonist from Streptomyces coelicolor (113 aa). O07728 No conserved hypotheticals O07728 O07728 GO:0006355,GO:0045152 O07728 NC_000962.3 Mycobrowser_v5 CDS 2151433 2152395 . - 0 Rv1905c aao Wide specificity for D-amino acids. Also acts on glycine [catalytic activity: a D-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2] Probable D-amino acid oxidase Aao Rv1905c, (MTCY180.13), len: 320 aa. Probable aao, D-amino acid oxidase, similar to many. Equivalent to AJ000521|MLCOSL672.02|O33145 Mycobacterium leprae (320 aa), FASTA results: opt: 1541, E(): 0, (71.7% identity in 315 aa overlap); also similar to OXDD_BOVIN|P31228 d-aspartate oxidase from bos taurus (338 aa), FASTA results: opt: 461, E(): 1.1e-21, (31.8% identity in 321 aa overlap). P9WP27 No intermediary metabolism and respiration O07727 P9WP27 GO:0055114,GO:0003884 1.4.3.3 P9WP27 NC_000962.3 Mycobrowser_v5 CDS 2152425 2152895 . - 0 Rv1906c Rv1906c Function unknown Conserved protein Rv1906c, (MTCY180.12), len: 156 aa. Conserved protein, possibly exported protein, equivalent to Mycobacterium leprae AJ000521|MLCOSL672.01 (153 aa), FASTA scores: opt: 637, E(): 2.6e-28, (63.2% identity in 155 aa overlap). Also similar to M. tuberculosis hypothetical exported protein, Rv1352. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). O07726 No conserved hypotheticals O07726 NC_000962.3 Mycobrowser_v5 CDS 2200726 2200941 . + 0 Rv1952 vapB14 Unknown Possible antitoxin VapB14 Rv1952, (MTCY09F9.12c), len: 71 aa. Possible vapB14, antitoxin, part of toxin-antitoxin (TA) operon with Rv1953 (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in M. tuberculosis e.g. Rv2601A. Some similarity to P55510|Y4JJ_RHISN putative plasmid stability protein (85 aa), FASTA scores: opt: 127, E(): 0.00096, (42.5% identity in 73 aa overlap). P95262 No virulence, detoxification, adaptation P95262 P95262 NC_000962.3 Mycobrowser_v5 CDS 2267119 2267724 . - 0 Rv2022c Rv2022c Unknown Conserved protein Rv2022c, (MTV018.09c), len: 201 aa. Conserved protein, similar to many. O53468 No conserved hypotheticals O53468 O53468 NC_000962.3 Mycobrowser_v5 CDS 2156149 2156592 . - 0 Rv1909c furA Acts as a global negative controlling element, employing FE(2+) as a cofactor to bind the operator of the repressed genes. Seems to regulate transcription of KATG|Rv1908c gene. Ferric uptake regulation protein FurA (fur) Rv1909c, (MTCY180.09), len: 147 aa. FurA, Ferric uptake regulation protein, similar to Q48835 legionella pneumophila 130B (wadsworth) ferric uptake regulation (136 aa), FASTA results: opt: 230, E(): 2.5e-09, (32.3% identity in 133 aa overlap). Also similar to Mycobacterium tuberculosis zur zinc uptake regulatory protein, Rv2359. Belongs to the fur family. Start changed since original submission (-3 aa). P9WN87 No regulatory proteins P0A582 P9WN87 GO:0046872,GO:0006355,GO:0006350,GO:0003700,GO:0005737 INH P9WN87 NC_000962.3 Mycobrowser_v5 CDS 2159191 2159943 . + 0 Rv1913 Rv1913 Unknown Conserved hypothetical protein Rv1913, (MTCY180.05c), len: 250 aa. Conserved hypothetical protein, slight similarity to dehydrase and beta-lactamase precursors e.g. Q02057 dehydrase from Streptomyces coelicolor (297 aa), FASTA scores: opt: 184, E(): 4.3e-05, (31.6% identity in 215 aa overlap). O07720 No conserved hypotheticals O07720 GO:0016787 O07720 NC_000962.3 Mycobrowser_v5 CDS 2160463 2161566 . + 0 Rv1915 aceAa Involved in glyoxylate bypass, an alternative to the tricarboxylic acid cycle [catalytic activity: isocitrate = succinate + glyoxylate]. Probable isocitrate lyase AceAa [first part] (isocitrase) (isocitratase) (Icl) Rv1915, (MTCY180.03c), len: 367 aa. Probable aceAa, isocitrate lyase (see citations below). Highly similar to the N-terminus of ACEA_MYCLE isocitrate lyase from Mycobacterium leprae (606 aa), FASTA results: opt: 3314, E(): 0, (86.5% identity in 572 aa overlap). Contains PS00161 Isocitrate lyase signature. Although this ORF and the downstream ORF representing the C-terminal half of aceA could be joined by a frameshift, no error is apparent in the cosmid, or in a seqencing read from the genome of H37Rv. As the downstream ORF has a RBS and transcriptional start immediately following the stop of this ORF, it is possible that they are expressed as two separate modules. In Mycobacterium tuberculosis strain CDC1551, aceA exists as a single gene, MT1966: the corresponding protein has been purified experimentally and seems have an active isocitrate lyase activity (see Honer et al., 1999). For Mycobacterium tuberculosis strain H37Rv, immunoblot assay didn't detect AceAa or AceAb products (see Honer et al., 1999) but mRNA of AceAa|Rv1915 has been detected (see Betts et al., 2002); so AceAb|Rv1916 could be a pseudogene. Icl2 has 2-methyl-isocitrate lyase (MCL) activity in M. tuberculosis Erdman (See Munoz-Elias et al., 2006). O07718 No intermediary metabolism and respiration O07718 O07718 GO:0004451,GO:0019752 4.1.3.1 O07718 NC_000962.3 Mycobrowser_v5 CDS 2162932 2167311 . - 0 Rv1917c PPE34 Function unknown PPE family protein PPE34 Rv1917c, (MTV050.01c-MTCY180.01), len: 1459 aa. PPE34, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, MPTR subfamily (see citation below). Similar to MTCY28.16, MTCY13E10.17, MTCY63.10, MTV004.05, MTCY98.24, MTCY6G11.05, etc. C-terminus is identical to Q50471. Unknown Mycobacterium tuberculosis protein (693 aa), FASTA results: opt: 2635, E(): 0, (99.7% identity in 391 aa overlap). Start changed since original submission (+23 aa). Thougth to be surface exposed, cell-wall associated. Q79FI9 No PE/PPE Q79FI9 Q79FI9 Q79FI9 NC_000962.3 Mycobrowser_v5 CDS 2167649 2170612 . - 0 Rv1918c PPE35 Function unknown PPE family protein PPE35 Rv1918c, (MTV050.02c), len: 987 aa. PPE35, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins. Similar to MTCY28.16|Z95890 Mycobacterium tuberculosis cosmid (1053 aa), FASTA scores: opt: 3404, E(): 0, (65.6% identity in 1058 aa overlap). Also similar to MTV004.05, MTY13E10.17, MTV014.03, MTCY3C7.23, MTCY6G11.05, MTCY48.17, MTV004.03, MTCY31.07, MTCY4C12.36, MTCY180.01, etc. Q79FI8 No PE/PPE Q79FI8 Q79FI8 Q79FI8 NC_000962.3 Mycobrowser_v5 CDS 2183866 2184957 . - 0 Rv1933c fadE18 Function unknown, but supposed involvement in lipid degradation. Probable acyl-CoA dehydrogenase FadE18 Rv1933c, (MTCY09F9.31), len: 363 aa. Probable fadE18, acyl-CoA dehydrogenase, similar to many e.g. CAB61609.1|AL133210 putative acyl-CoA dehydrogenase from Streptomyces coelicolor (362 aa); NP_421282.1|NC_002696 acyl-CoA dehydrogenase family protein from Caulobacter crescentus (344 aa); ACDS_RAT|P15651 short-chain specific acyl-CoA dehydrogenase from Rattus norvegicus (Rat) (412 aa), fasta scores: opt: 239, E(): 2.1e-08, (28.4% identity in 331 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. N-terminus of fadE22 (721 aa); fadE33 (318 aa); N-terminus of fadE34 (711 aa); etc. Could belong to the acyl-CoA dehydrogenases family. P95281 No lipid metabolism P95281 P95281 GO:0003995,GO:0055114,GO:0050660 1.3.99.- P95281 NC_000962.3 Mycobrowser_v5 CDS 2267749 2268108 . - 0 Rv2023c Rv2023c Unknown Hypothetical protein Rv2023c, (MTV018.10c), len: 119 aa. Hypothetical protein, alternative upstream start possible. O53469 No conserved hypotheticals O53469 O53469 NC_000962.3 Mycobrowser_v5 CDS 2282747 2283721 . - 0 Rv2037c Rv2037c Unknown Conserved transmembrane protein Rv2037c, (MTV018.24c), len: 324 aa. Conserved transmembrane protein, similar to many. Alternative nucleotide at position 2282787 (C->T; C312Y) has been observed. Contains IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase motif. L0TB61 No cell wall and cell processes O53481 L0TB61 GO:0006629,GO:0016021 L0TB61 NC_000962.3 Mycobrowser_v5 CDS 2172524 2173795 . - 0 Rv1921c lppF Unknown Probable conserved lipoprotein LppF Rv1921c, (MTCY09F9.43-MTV050.05c), len: 423 aa. Probable lppF, conserved lipoprotein, similar to G403173 lipoprotein precursor (fragment) from Rhodococcus erythropolis (225 aa), fasta scores: opt: 364, E(): 9.2e-19, (41.9% identity in 148 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O53963 No cell wall and cell processes O53963 O53963 NC_000962.3 Mycobrowser_v5 CDS 2175173 2176513 . + 0 Rv1923 lipD Function unknown; lipolytic enzyme probably involved in cellular metabolism Probable lipase LipD Rv1923, (MTCY09F9.41c), len: 446 aa. Probable lipD, hydrolase lipase, similar to esterases and beta-lactamases e.g. G151214 esterase, (389 aa), fasta scores: opt: 569, E(): 5.4e-29, (33.7% identity in 401 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins Rv1497, Rv2463, Rv3775, etc. P95290 No intermediary metabolism and respiration P95290 P95290 GO:0016787 3.1.-.- P95290 NC_000962.3 Mycobrowser_v5 CDS 2178957 2179436 . - 0 Rv1926c mpt63 Unknown Immunogenic protein Mpt63 (antigen Mpt63/MPB63) (16 kDa immunoprotective extracellular protein) Rv1926c, (MT1977, MTCY09F9.38), len: 159 aa. Mpt63 (alternate gene name: mpb63), immunogenic protein (see citations below), identical to MPT63|MPB63 from Mycobacterium bovis (159 aa). Exported protein containing a N-terminal signal sequence: see notes below about proteomics. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WIP1 No cell wall and cell processes 1LMI P0A5Q2 P9WIP1 GO:0005615 P9WIP1 NC_000962.3 Mycobrowser_v5 CDS 2182460 2183239 . - 0 Rv1931c Rv1931c Involved in transcriptional mechanism Probable transcriptional regulatory protein Rv1931c, (MTCY09F9.33), len: 259 aa. Probable transcriptional regulatory protein. Similarity in C-terminal half to transcriptional activators e.g. Q43970 AraC-like protein (227 aa), FASTA scores: opt: 238, E(): 7.1e-07, (42.4% identity in 92 aa overlap). Similar to many probable transcription regulators in Streptomyces e.g. AL049587|SC5F2A.29 Streptomyces coelicolor (325 aa), FASTA scores: opt: 387, E(): 3.2e-16, (34.4% identity in 259 aa overlap). P95283 No regulatory proteins P95283 P95283 GO:0006355,GO:0043565,GO:0006350,GO:0003700,GO:0005622 P95283 NC_000962.3 Mycobrowser_v5 CDS 2294531 2306986 . - 0 Rv2048c pks12 Involved in biosynthesis of mannosyl-beta-1-phosphomycoketide (MPM) Polyketide synthase Pks12 Rv2048c, (MTV018.35c), len: 4151 aa. Pks12, polyketide synthase similar to many. Contains 2x PS00012 Phosphopantetheine attachment site, 2x PS00606 Beta-ketoacyl synthases active site, and PS00343 Gram-positive cocci surface proteins 'anchoring' hexapeptide. Nucleotide position 2297976 in the genome sequence has been corrected, G:A resulting in S3004L. I6XD69 No lipid metabolism O53490 I6XD69 GO:0009058,GO:0048037,GO:0055114,GO:0016491,GO:0031177,GO:0016740,GO:0008270,GO:0000036 I6XD69 NC_000962.3 Mycobrowser_v5 CDS 2181262 2181906 . - 0 Rv1929c Rv1929c Function unknown Conserved hypothetical protein Rv1929c, MTCY09F9.35, len: 214 aa. Conserved hypothetical protein, similar to SC4G6.14|AL096884 hypothetical protein from Streptomyces coelicolor (211 aa), FASTA scores: opt: 416, E(): 2.4e-22, (39.8% identity in 206 aa overlap). P95285 No conserved hypotheticals P95285 P95285 NC_000962.3 Mycobrowser_v5 CDS 2181918 2182442 . - 0 Rv1930c Rv1930c Function unknown Conserved hypothetical protein Rv1930c, MTCY09F9.34, len: 174 aa. Conserved hypothetical protein, similar to SC5F2A.30|AL049587 hypothetical protein from Streptomyces coelicolor (211 aa), FASTA scores: opt: 307, E(): 2.8e-13, (54.8% identity in 84 aa overlap). Some similarity to M. tuber culosis hypothetical protein Rv0052|MTCY21D4.15 (43% identity in 93 aa overlap). P95284 No conserved hypotheticals P95284 P95284 NC_000962.3 Mycobrowser_v5 CDS 2186203 2187159 . - 0 Rv1935c echA13 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Possible enoyl-CoA hydratase EchA13 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv1935c, (MTCY09F9.29), len: 318 aa. Possible echA13, enoyl-CoA hydratase, similar to others and various enzymes e.g. CAC48381.1|Y16952 putative enoyl-CoA-isomerase from Amycolatopsis mediterranei (269 aa); AAK18173.1|AF290950_5|AF290950|FadB1x enoyl-CoA hydratase from Pseudomonas putida (257 aa); AAF78820.1|AF042490 4-chlorobenzoyl CoA dehalogenase from Arthrobacter sp. TM1 (276 aa); ECHM_RAT|P14604 enoyl-CoA hydratase mitochondrial precursor from Rattus norvegicus (Rat) (290 aa), FASTA scores: opt: 228, E(): 1.2e-08, (31.0% identity in 258 aa overlap); etc. P95279 No lipid metabolism P95279 P95279 GO:0016853,GO:0008152,GO:0004300 4.2.1.17 P95279 NC_000962.3 Mycobrowser_v5 CDS 2187384 2188493 . + 0 Rv1936 Rv1936 Function unknown; probably involved in cellular metabolism Possible monooxygenase Rv1936, (MTCY09F9.28c), len: 369 aa. Possible monooxygenase, similar to LXA2_PHOLU|P23146 alkanal monooxygenase alpha chain (362 aa), FASTA scores: opt: 196, E(): 6.3e-06, (22.3% identity in 373 aa overlap). Also similar to many other Mycobacterium tuberculosis hypothetical oxidoreductases and monooxygenases e.g. Rv0953c, Rv0791c, Rv0132c, etc. P95278 No intermediary metabolism and respiration P95278 P95278 GO:0055114,GO:0004497 1.-.-.- P95278 NC_000962.3 Mycobrowser_v5 CDS 2216592 2217167 . + 0 Rv1972 Rv1972 Unknown Probable conserved Mce associated membrane protein Rv1972, (MTV051.10), len: 191 aa. Probable conserved Mce-associated membrane protein. Probably part of mce3 operon. Similar to several Mycobacterium tuberculosis proteins e.g. Rv1363c|Z75555|MTCY02B10.27C (261 aa), FASTA scores: opt: 342, E(): 1.2e-15, (31.8% identity in 195 aa overlap); Rv1362c, Rv0177 (near Mce operon 1), etc. Has hydrophobic stretch at aa 20-40. O53973 No cell wall and cell processes O53973 O53973 NC_000962.3 Mycobrowser_v5 ncRNA 2299745 2299886 . + 0 MTB000057 ASpks Unknown Putative small regulatory RNA ASpks, putative small regulatory RNA (See Arnvig and Young, 2009). Alternate 5'-ends at positions 2299785 and 2299796. Alternate 3'-end at position 2299873. This sequence is repeated in pks12|Rv2048c at position 2305814-2305955. No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 2245209 2246822 . + 0 Rv2000 Rv2000 Unknown Unknown protein Rv2000, (MTCY39.18c), len: 537 aa. Unknown protein. P9WLN7 No conserved hypotheticals P9WLN7 NC_000962.3 Mycobrowser_v5 CDS 2191027 2192097 . + 0 Rv1938 ephB This enzyme acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potential toxic epoxides. Also determines steady-state levels of physiological mediators. Probable epoxide hydrolase EphB (epoxide hydratase) Rv1938, (MTCY09F9.26c), len: 356 aa. Probable ephB, epoxide hydrolase (see citation below), similar to many e.g. G1109600 ATSEH (321 aa), FASTA scores: opt: 442, E(): 1.2e-21 (33.1% identity in 356 aa overlap); etc. Also similar to many other M. tuberculosis hypothetical epoxide hydrolases e.g. Rv3617, Rv3670, Rv0134, etc. I6YC03 No virulence, detoxification, adaptation 2ZJF,2E3J P95276 I6YC03 GO:0016787 3.3.2.3 I6YC03 NC_000962.3 Mycobrowser_v5 CDS 2192606 2193667 . + 0 Rv1940 ribA1 Involved in riboflavin biosynthesis [catalytic activity: GTP + 3 H(2)O = formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate] Probable riboflavin biosynthesis protein RibA1 (GTP cyclohydrolase II) Rv1940, (MTCY09F9.24c), len: 353 aa. Probable ribA1, Riboflavin biosynthesis protein, similar to GCH2_BACSU|P17620 gtp cyclohydrolase II (398 aa), FASTA scores: opt: 682, E(): 0, (37.7% identity in 363 aa overlap), also similar to Rv1415|MTCY21B4.33|ribA2 (428 aa) (45.4% identity in 368 aa overlap). Note that previously known as ribA. L7N669 No intermediary metabolism and respiration Q7D7R0 L7N669 GO:0003935,GO:0009231,GO:0008686 3.5.4.25 L7N669 NC_000962.3 Mycobrowser_v5 CDS 2193664 2194434 . + 0 Rv1941 Rv1941 Function unknown; probably involved in cellular metabolism Probable short-chain type dehydrogenase/reductase Rv1941, (MTCY09F9.23c), len: 256 aa. Probable short-chain dehydrogenase/reductase, similar to various dehydrogenases/reductases, generally belonging to SDR family, e.g. NP_299015.1|NC_002488 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase from Xylella fastidiosa (255 aa); NP_250340.1|NC_002516 probable short-chain dehydrogenase from Pseudomonas aeruginosa (253 aa); NP_106890.1|NC_002678 probable short-chain type dehydrogenase/reductase from Mesorhizobium loti (374 aa) (has its N-terminus longter); P50197|LINC_PSEPA 2,5-dichloro-2,5-cyclohexadiene-1,4-dehydrogenase from Pseudomonas paucimobilis (Sphingomonas paucimobilis) (250 aa), FASTA scores: opt: 529, E(): 5.7e-25, (40.6% identity in 251 aa overlap); etc. Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. I6XZC4 No intermediary metabolism and respiration 3GVC P95273 I6XZC4 GO:0055114,GO:0016491 1.-.-.- I6XZC4 NC_000962.3 Mycobrowser_v5 CDS 2195989 2197353 . + 0 Rv1945 Rv1945 Unknown Conserved hypothetical protein Rv1945, (MTCY09F9.19c), len: 454 aa. Member of Mycobacterium tuberculosis REP13E12 repeat family. Similar to several others, best with Rv1148c|Z95584|MTCI65.15 (482 aa), FASTA score: opt: 2954, E(): 0, (97.1% identity in 454 aa overlap). Contains possible helix-turn-helix motif at aa 74-95 (+2.90 SD). P9WLQ5 No insertion seqs and phages P95269 P9WLQ5 P9WLQ5 NC_000962.3 Mycobrowser_v5 CDS 2555941 2556135 . + 0 Rv2283 Rv2283 Unknown Hypothetical protein Rv2283, (MTCY339.27c), len: 64 aa. Unknown protein; questionable ORF. P9WLE9 No conserved hypotheticals P9WLE9 NC_000962.3 Mycobrowser_v5 CDS 2203974 2204225 . - 0 Rv1960c parD1 Function unknown Possible antitoxin ParD1 Rv1960c, (MTCY09F9.04), len: 83 aa. Possible parD1, antitoxin, part of toxin-antitoxin (TA) operon with Rv1959c (See Pandey and Gerdes, 2005), similar to O85269|AF102990|AF102990_51 hypothetical protein of Yersinia enterocolitica (80 aa), FASTA scores: opt: 149, E(): 0.00037, (42.1% identity in 57 aa overlap). P9WIJ7 No virulence, detoxification, adaptation P9WIJ7 GO:0045449 P9WIJ7 NC_000962.3 Mycobrowser_v5 CDS 2197508 2197960 . - 0 Rv1946c lppG Unknown Possible lipoprotein Rv1946c, (MTCY09F9.18), len: 150 aa. Possible lppG, conserved lipoprotein, showing some similarity to Rv1943c|MTCY09F9.21 conserved hypothetical protein from Mycobacterium tuberculosis (125 aa), FASTA score: (71.4% identity in 28 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P95268 No cell wall and cell processes P95268 NC_000962.3 Mycobrowser_v5 CDS 2198024 2198425 . + 0 Rv1947 Rv1947 Unknown Hypothetical protein Rv1947, (MTCY09F9.17c), len: 133 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P95267 No conserved hypotheticals P95267 NC_000962.3 Mycobrowser_v5 CDS 2198714 2199064 . - 0 Rv1948c Rv1948c Unknown Hypothetical protein Rv1948c, (MTCY09F9.16), len: 116 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P95266 No conserved hypotheticals P95266 NC_000962.3 Mycobrowser_v5 CDS 2231680 2232219 . + 0 Rv1988 erm(37) Thought to cause methylation of 23S rRNA Probable 23S rRNA methyltransferase Erm(37) Rv1988, (MTCY39.31c), len: 179 aa. Probable erm(37), 23S rRNA methyltransferase, similar to ERME_SACER|P07287 rrna adenine n-6-methyltransferase (370 aa), FASTA scores: opt: 259, E(): 2e-11, (35.1% identity in 171 aa overlap); contains PS00092 N-6 Adenine-specific DNA methylases signature. Also similar to Mycobacterium tuberculosis Rv1010 ksgA 16S rRNA dimethyltransferase. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q10838 No intermediary metabolism and respiration Q10838 Q10838 GO:0000154,GO:0000179 2.1.1.- Q10838 NC_000962.3 Mycobrowser_v5 CDS 2199075 2200034 . - 0 Rv1949c Rv1949c Function unknown Conserved hypothetical protein Rv1949c, (MTCY09F9.15), len: 319 aa. Conserved hypothetical protein, partial ORF. Rv1949c and Rv1950c|MTCY09F9.14 are similar but frameshifted with respect to Rv2077c|MTCY49.16C|Q10685 hypothetical 33.3 kd protein (323 aa), FASTA scores: opt: 459, E(): 2.8e-16, (54.8% identity in 157 aa overlap). Cosmid sequence appears to be correct, genomic sequence is also frameshifted in Mycobacterium bovis strain AF2122/97. Similar to Mycobacterium tuberculosis hypothetical proteins: Rv2542, Rv2077c, Rv2797c, Rv0963c, etc. L0T9Q6 No conserved hypotheticals L0T9Q6 L0T9Q6 NC_000962.3 Mycobrowser_v5 CDS 2199998 2200189 . - 0 Rv1950c Rv1950c Function unknown Conserved hypothetical protein Rv1950c, (MTCY09F9.14), len: 63 aa. Conserved hypothetical protein, partial ORF. Highly similar to N-terminus of Rv2077c|MTCY49.16C|Q10685 hypothetical 33.3 kDa protein (323 aa), FASTA scores: opt: 280, E(): 1.2 e-16, (71.7% identity in 53 aa overlap) but homology continues in different frame ie MTCY09F9.15, cosmid sequence appears to be correct, genomic sequence is also frameshifted in Mycobacterium bovis strain AF2122/97. P95264 No conserved hypotheticals P95264 NC_000962.3 Mycobrowser_v5 CDS 2201719 2202096 . + 0 Rv1955 higB Unknown Possible toxin HigB Rv1955, (MTCY09F9.09c), len: 125 aa. Possible higB, toxin, part of toxin-antitoxin (TA) operon with Rv1956 (See Pandey and Gerdes, 2005; Gupta, 2009). Start overlaps another ORF, Rv1954c (MTCY09F9.10). Start changed since first submission (-45 aa). Predicted to be an outer membrane protein (See Song et al., 2008). Upon expression in E. coli has been shown to function as an antitoxin against Rv1956 (PubMed: 19016878); It is not clear if these conflicting results are due to expression in a heterologous system; In various publications, both gene names higA and higB have been assigned to both Rv1955 and Rv1956; we have chosen to call Rv1955 higB after consulting the authors. P9WJA5 No virulence, detoxification, adaptation P95259 P9WJA5 P9WJA5 NC_000962.3 Mycobrowser_v5 CDS 2246832 2247584 . + 0 Rv2001 Rv2001 Unknown Conserved hypothetical protein Rv2001, (MTCY39.17c), len: 250 aa. Conserved hypothetical protein. Similar to Mycobacterium tuberculosis Rv0466. P9WLN5 No conserved hypotheticals Q10856 P9WLN5 P9WLN5 NC_000962.3 Mycobrowser_v5 CDS 2203018 2203632 . - 0 Rv1958c Rv1958c Unknown Hypothetical protein Rv1958c, (MTCY09F9.06), len: 204 aa. Hypothetical unknown protein, questionable ORF P95256 No conserved hypotheticals P95256 P95256 NC_000962.3 Mycobrowser_v5 CDS 2203681 2203977 . - 0 Rv1959c parE1 Function unknown Possible toxin ParE1 Rv1959c, (MTCY09F9.05), len: 98 aa. Possible parE1, toxin, part of toxin-antitoxin (TA) operon with Rv1960c (See Pandey and Gerdes, 2005), similar to other hypothetical plasmid proteins e.g. AL117189|YPCD1.08 from Yersinia pestis (99 aa), FASTA scores: opt: 162, E(): 7.3e-05, (33.0% identity in 91 aa overlap); also some similarity to E145339 hypothetical protein (103 aa), FASTA scores: opt: 142, E(): 0.0003, (33.0% identity in 91 aa overlap). P9WHG7 No virulence, detoxification, adaptation P95255 P9WHG7 P9WHG7 NC_000962.3 Mycobrowser_v5 -35_signal 2207496 2207501 . + 0 Rv1964 yrbE3A Unknown Conserved hypothetical integral membrane protein YrbE3A Rv1964, (MTV051.02), len: 265 aa. YrbE3A, hypothetical unknown integral membrane protein, part of mce3 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07412|Rv0167|MTCI28.07|yrbE1A (265 aa), O07791|Rv0587|MTCY19H5.35|yrbE2A (265 aa), Rv3501c|MTV023.08c|yrbE4A (254 aa), etc. Also highly similar to conserved hypothetical integral membrane proteins of yrbEA type, e.g. AAD24544.1|AF116213|YrbE1A from Mycobacterium leprae (112 aa); P45392|YRBE_ECOLI from Escherichia coli (260 aa), FASTA scores: opt: 893, E(): 0, (51.4% identity in 253 aa overlap); etc. The transcription of this CDS seems negatively regulated by the product of Rv1963c|mce3R (see Santangelo et al., 2002). O53965 No virulence, detoxification, adaptation O53965 O53965 O53965 NC_000962.3 Mycobrowser_v5 -10_signal 2207518 2207523 . + 0 Rv1964 yrbE3A Unknown Conserved hypothetical integral membrane protein YrbE3A Rv1964, (MTV051.02), len: 265 aa. YrbE3A, hypothetical unknown integral membrane protein, part of mce3 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07412|Rv0167|MTCI28.07|yrbE1A (265 aa), O07791|Rv0587|MTCY19H5.35|yrbE2A (265 aa), Rv3501c|MTV023.08c|yrbE4A (254 aa), etc. Also highly similar to conserved hypothetical integral membrane proteins of yrbEA type, e.g. AAD24544.1|AF116213|YrbE1A from Mycobacterium leprae (112 aa); P45392|YRBE_ECOLI from Escherichia coli (260 aa), FASTA scores: opt: 893, E(): 0, (51.4% identity in 253 aa overlap); etc. The transcription of this CDS seems negatively regulated by the product of Rv1963c|mce3R (see Santangelo et al., 2002). O53965 No virulence, detoxification, adaptation O53965 O53965 O53965 NC_000962.3 Mycobrowser_v5 CDS 2207700 2208497 . + 0 Rv1964 yrbE3A Unknown Conserved hypothetical integral membrane protein YrbE3A Rv1964, (MTV051.02), len: 265 aa. YrbE3A, hypothetical unknown integral membrane protein, part of mce3 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07412|Rv0167|MTCI28.07|yrbE1A (265 aa), O07791|Rv0587|MTCY19H5.35|yrbE2A (265 aa), Rv3501c|MTV023.08c|yrbE4A (254 aa), etc. Also highly similar to conserved hypothetical integral membrane proteins of yrbEA type, e.g. AAD24544.1|AF116213|YrbE1A from Mycobacterium leprae (112 aa); P45392|YRBE_ECOLI from Escherichia coli (260 aa), FASTA scores: opt: 893, E(): 0, (51.4% identity in 253 aa overlap); etc. The transcription of this CDS seems negatively regulated by the product of Rv1963c|mce3R (see Santangelo et al., 2002). O53965 No virulence, detoxification, adaptation O53965 O53965 O53965 NC_000962.3 Mycobrowser_v5 CDS 2217164 2217646 . + 0 Rv1973 Rv1973 Unknown Possible conserved Mce associated membrane protein Rv1973, (MTV051.11), len: 160 aa. Possible conserved Mce-associated membrane protein. Probably part of mce3 operon. Similar to several other proteins from Mycobacterium tuberculosis e.g. Rv1362c|Z75555|MTCY02B10.26C (220 aa), FASTA scores: opt: 378, E(): 2.8e-19, (50.0% identity in 128 aa overlap); Rv1363c; Rv0177 (near Mce operon 1); etc. Contains possible N-terminal signal sequence or membrane anchor. Predicted to be an outer membrane protein (See Song et al., 2008). P9WJ77 No cell wall and cell processes P9WJ77 P9WJ77 NC_000962.3 Mycobrowser_v5 CDS 2238141 2238908 . + 0 Rv1995 Rv1995 Unknown Unknown protein Rv1995, (MTCY39.24c), len: 255 aa. Unknown protein. P9WLP3 No conserved hypotheticals P64915 P9WLP3 P9WLP3 NC_000962.3 Mycobrowser_v5 CDS 2591848 2592726 . - 0 Rv2319c Rv2319c Unknown Universal stress protein family protein Rv2319c, (MTCY3G12.15), len: 292 aa. Universal stress protein family protein. P9WLB5 No virulence, detoxification, adaptation P64995 P9WLB5 GO:0006950 P9WLB5 NC_000962.3 Mycobrowser_v5 CDS 2210601 2211629 . + 0 Rv1967 mce3B Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce3B Rv1967, (MTV051.05), len: 342 aa. Mce3B; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07414|Rv0170|MTCI28.10|mce1B (346 aa); O07788|Rv0590|MTCY19H5.32c|mce2B (275 aa); etc. Also similar to others e.g. NP_302657.1|NC_002677 putative secreted protein from Mycobacterium leprae (346 aa); CAC12797.1|AL445327 putative secreted protein from Streptomyces coelicolor (354 aa); etc. Contains a possible N-terminal signal sequence or membrane anchor. The transcription of this CDS seems negatively regulated by the product of Rv1963c|mce3R (see Santangelo et al., 2002). Predicted to be an outer membrane protein (See Song et al., 2008). O53968 No virulence, detoxification, adaptation O53968 O53968 O53968 NC_000962.3 Mycobrowser_v5 CDS 2212855 2214126 . + 0 Rv1969 mce3D Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce3D Rv1969, (MTV051.07), len: 423 aa. Mce3D; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07416|Rv0172|MTCI28.12|mce1D (530 aa); O07786|Rv0592|MTCY19H5.30c|mce2D (508 aa); etc. Also highly similar to others e.g. NP_302659.1|NC_002677 putative secreted protein from Mycobacterium leprae (531 aa); CAC12795.1|AL445327 putative secreted protein from Streptomyces coelicolor (337 aa); etc. Contains a possible N-terminal signal sequence or membrane anchor. The transcription of this CDS seems negatively regulated by the product of Rv1963c|mce3R (see Santangelo et al., 2002). Predicted to be an outer membrane protein (See Song et al., 2008). O53970 No virulence, detoxification, adaptation O53970 O53970 O53970 NC_000962.3 Mycobrowser_v5 CDS 2214123 2215256 . + 0 Rv1970 lprM Unknown, but thought to be involved in host cell invasion. Possible Mce-family lipoprotein LprM (Mce-family lipoprotein Mce3E) Rv1970, (MTV051.08), len: 377 aa. Possible lprM (alternate gene name: mce3E), lipoprotein which belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07417|LPRK|Rv0173|MTCI28.13|mce1E (390 aa); O07785|LPRL|Rv0593|MTCY19H5.29|mce2E (402 aa); etc. Also highly similar to others e.g. NP_302660.1|NC_002677 putative lipoprotein from Mycobacterium leprae (392 aa); CAC12794.1|AL445327 putative secreted protein from Streptomyces coelicolor (413 aa); etc. Contains possible N-terminal signal sequence or membrane anchor and PS00013 Prokaryotic membrane lipoprotein lipid attachment site. The transcription of this CDS seems negatively regulated by the product of Rv1963c|mce3R (see Santangelo et al., 2002). O53971 No cell wall and cell processes O53971 O53971 O53971 NC_000962.3 Mycobrowser_v5 CDS 2215257 2216570 . + 0 Rv1971 mce3F Unknown, but thought involved in host cell invasion. Mce-family protein Mce3F Rv1971, (MTV051.09), len: 437 aa. Mce3F; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), similar to Mycobacterium tuberculosis proteins O07418|Rv0174|MTCI28.14|mce1F (515 aa), O07784|Rv0594|MTCY19H5.28c|mce2F (516 aa); etc. Also highly similar to others e.g. NP_302661.1|NC_002677 putative secreted protein from Mycobacterium leprae (516 aa); CAC12793.1|AL445327 putative secreted protein from Streptomyces coelicolor (433 aa); etc. Contains a possible N-terminal signal sequence or membrane anchor. The transcription of this CDS seems negatively regulated by the product of Rv1963c|mce3R (see Santangelo et al., 2002). Predicted to be an outer membrane protein (See Song et al., 2008). O53972 No virulence, detoxification, adaptation O53972 O53972 O53972 NC_000962.3 Mycobrowser_v5 CDS 2227908 2228561 . - 0 Rv1984c cfp21 Hydrolyzes cutin. Shown to have esterase and lipase activity. Probable cutinase precursor CFP21 Rv1984c, (MTCY39.35), len: 217 aa. Cfp21, probable cutinase precursor with N-terminal signal sequence, similar to P41744|CUTI_ALTBR cutinase precursor from Alternaria brassicicola (209 aa), FASTA scores: opt: 283, E(): 2.2e-11, (32.6% identity in 193 aa overlap). Also similar to Mycobacterium tuberculosis proteins e.g. Rv3452, Rv3451, Rv2301, Rv1758, Rv3724. Belongs to the cutinase family. P9WP43 No cell wall and cell processes P63879 P9WP43 GO:0005576,GO:0008152,GO:0050525 3.1.1.74 P9WP43 NC_000962.3 Mycobrowser_v5 CDS 2220908 2221756 . + 0 Rv1978 Rv1978 Function unknown Conserved protein Rv1978, (MTV051.16), len: 282 aa. Conserved protein, similar to several hypothetical proteins and methyltransferases e.g. X86780|SHGCPIR.15 methyltransferase from S. hygroscopicus (211 aa), FASTA scores: opt: 151, E(): 0.0072, (30.6% identity in 121 aa overlap). O53979 No conserved hypotheticals O53979 O53979 O53979 NC_000962.3 Mycobrowser_v5 CDS 2232739 2233299 . - 0 Rv1989c Rv1989c Unknown Hypothetical protein Rv1989c, (MTCY39.30), len: 186 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLP9 No conserved hypotheticals P64907 P9WLP9 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2221719 2223164 . - 0 Rv1979c Rv1979c Unknown; possibly involved in transport of amino acid across the membrane. Possible conserved permease Rv1979c, (MTCY39.40-MTV051.17c), len: 481 aa. Possible permease, APC family possibly involved in transport of amino acid, showing some similarity to other permeases. Also similar to MTCY39.19 from Mycobacterium tuberculosis (28.2% identity in 277 aa overlap). Contains PS00599 Aminotransferases class-II pyridoxal-phosphate attachment site. Nucleotide position 2221796 in the genome sequence has been corrected, C:T resulting in V457I. P9WQM5 No cell wall and cell processes Q10875 P9WQM5 GO:0006865,GO:0016021,GO:0005886,GO:0055085,GO:0015171 P9WQM5 NC_000962.3 Mycobrowser_v5 CDS 2223343 2224029 . - 0 Rv1980c mpt64 Unknown Immunogenic protein Mpt64 (antigen Mpt64/MPB64) Rv1980c, (MT2032, MTCY39.39), len: 228 aa. Mpt64 (alternate gene name: mpb64), immunogenic protein (alternate gene name: mpb64) (see citations below), identical to MPT64|MPB64 from Mycobacterium bovis (228 aa). Similar to Rv3036c|MTV012.51c from Mycobacterium tuberculosis. Exported protein containing a N-terminal signal sequence: see notes below about proteomics. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WIN9 No cell wall and cell processes 2HHI P0A5Q4 P9WIN9 GO:0005576 P9WIN9 NC_000962.3 Mycobrowser_v5 CDS 2247660 2248442 . + 0 Rv2002 fabG3 Not really known; thought to be involved in lipid biosynthesis [catalytic activity: androstan-3-alpha,17-beta-DIOL + NAD+ = 17-beta-hydroxyandrostan-3-one + NADH]. Possible 20-beta-hydroxysteroid dehydrogenase FabG3 (cortisone reductase) ((R)-20-hydroxysteroid dehydrogenase) Rv2002, (MTCY39.16c), len: 260 aa. FabG3, 20-beta-hydroxysteroid dehydrogenase. Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. P9WGT1 No lipid metabolism 1NFF,1NFQ,1NFR P69167 P9WGT1 GO:0055114,GO:0008202,GO:0047044 1.1.1.53 P9WGT1 NC_000962.3 Mycobrowser_v5 CDS 2252002 2255985 . + 0 Rv2006 otsB1 Involved in trehalose biosynthesis (protective effect). Mycobacteria can produce trehalose from glucose 6-phosphate and UDP-glucose (the OtsA-OtsB pathway) from glycogen-like alpha(1-->4)-linked glucose polymers (the TreY-TreZ pathway) and from maltose (the TreS pathway) [catalytic activity: trehalose 6-phosphate + H(2)O = trehalose + orthophosphate]. Probable trehalose-6-phosphate phosphatase OtsB1 (trehalose-phosphatase) (TPP) Rv2006, (MTCY39.11c), len: 1327 aa. OtsB1, trehalose-6-phosphate phosphatase (see citations below). Belongs to Glycosyl hydrolases family 65. Note that previously known as otsB. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WN15 No virulence, detoxification, adaptation Q10850 P9WN15 GO:0016798,GO:0005992,GO:0030246 3.2.1.- P9WN15 NC_000962.3 Mycobrowser_v5 CDS 2945847 2946287 . - 0 Rv2617c Rv2617c Unknown Probable transmembrane protein Rv2617c, (MTCY01A10.17), len: 146 aa. Probable transmembrane protein, showing some similarity to hypothetical or membrane proteins e.g. CAC47207|SMC00744 putative transport protein transmembrane from Rhizobium meliloti (Sinorhizobium meliloti) (399 aa), FASTA scores: opt: 108, E(): 5.5, (29.15% identity in 144 aa overlap). I6XER9 No cell wall and cell processes O06197 I6XER9 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2228991 2229902 . - 0 Rv1985c Rv1985c Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably LysR-family) Rv1985c, (MTCY39.34), len: 303 aa. Probable transcriptional regulatory protein, LysR family member. Similar to many regulatory proteins, especially ICIA_ECOLI|P24194 chromosome initiation inhibitor from Escherichia coli (297 aa), FASTA scores: opt: 520, E(): 1.1e-28, (35.8% identity in 285 aa overlap); and P94632|LYSG_CORGL lysine export regulator protein (290 aa), FASTA scores: opt: 705, E(): 0, (42.7% identity in 288 aa overlap); etc. Contains PS00044 Bacterial regulatory proteins, lysR family signature. Also contains helix-turn-helix motif at aa 22-43,(+5.52 SD). Belongs to the LysR family of transcriptional regulators. P9WMF5 No regulatory proteins 3ISP P67665 P9WMF5 GO:0006350,GO:0003700,GO:0006355 P9WMF5 NC_000962.3 Mycobrowser_v5 CDS 2230011 2230610 . + 0 Rv1986 Rv1986 Unknown; possibly involved in transport of lysine across the membrane. Probable conserved integral membrane protein Rv1986, (MTCY39.33c), len: 199 aa. Probable conserved integral membrane protein, LysE family possibly involved in transport of Lysine, similar to P11667|YGGA_ECOLI hypothetical 23.2 kDa protein in sbm-fba intergenic region (211 aa), FASTA scores: opt: 379, E(): 1.5e-19, (37.3% identity in 185 aa overlap); and Q11154|Rv0488 hypothetical 20.9 kDa protein from M. tuberculosis (201 aa), FASTA scores: opt: 784, E(): 0, (63.4% identity in 186 aa overlap). Belongs to the LYSE/YGGA family. P9WK31 No cell wall and cell processes P64903 P9WK31 GO:0016021,GO:0005886,GO:0006865 NC_000962.3 Mycobrowser_v5 CDS 2231026 2231454 . + 0 Rv1987 Rv1987 Hydrolysis of chitin Possible chitinase Rv1987, (MTCY39.32c), len: 142 aa. Possible chitinase, similar to several e.g. P36909|CHIT_STRLI chitinase c precursor (619 aa) FASTA scores, opt: 324, E(): 1.2e-14, (39.5% identity in 129 aa overlap). P9WLQ1 No cell wall and cell processes P64905 P9WLQ1 GO:0004553,GO:0030247,GO:0005975 P9WLQ1 NC_000962.3 Mycobrowser_v5 CDS 2233296 2233637 . - 0 Rv1990c Rv1990c Involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv1990c, (MTCY39.29), len: 113 aa. Probable transcriptional regulatory protein, similar to Mycobacterium tuberculosis Rv3188|AL021646|MTV014.32 (115 aa), FASTA scores: opt: 184, E(): 8.2e-07, (28.4% identity in 109 aa overlap). Contains probable helix-turn-helix motif at aa 20-44 (+4.22 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLP7 No regulatory proteins P9WLP7 NC_000962.3 Mycobrowser_v5 CDS 2233881 2234216 . - 0 Rv1990A Rv1990A Function unknown; probably involved in cellular metabolism. Possible dehydrogenase (fragment) Rv1990A, len: 111 aa. Possible dehydrogenase (fragment), similar to N-terminal part of several dehydrogenases and hypothetical proteins, e.g. Rv2750|MTV002.15|AL008967 from Mycobacterium tuberculosis (272 aa), FASTA scores: opt: 151, E(): 0.0045, (47.45% identity in 78 aa overlap), but lacks C-terminal part. Maybe a pseudogene. Also similar to U17129|RSU17129_7 putative short-chain alcohol dehydrogenase from Rhodococcus erythropolis (275 aa), FASTA scores: opt: 142, E(): 0.018, (54.15% identity in 48 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L7N6A3 No intermediary metabolism and respiration L7N6A3 GO:0055114,GO:0016491 1.-.-.- L7N6A3 NC_000962.3 Mycobrowser_v5 CDS 2248563 2249420 . - 0 Rv2003c Rv2003c Unknown Conserved hypothetical protein Rv2003c, (MTCY39.14), len: 285 aa. Conserved hypothetical protein. P9WJZ5 No conserved hypotheticals P64919 P9WJZ5 GO:0008168,GO:0008152 P9WJZ5 NC_000962.3 Mycobrowser_v5 CDS 2321057 2321467 . + 0 Rv2063A mazF7 Unknown Possible toxin MazF7 Rv2063A, len: 136 aa. Possible mazF7 toxin, part of toxin-antitoxin (TA) operon with Rv2063 (See Pandey and Gerdes, 2005). P0CL62 No virulence, detoxification, adaptation P0CL62 P0CL62 NC_000962.3 Mycobrowser_v5 CDS 2234305 2234649 . - 0 Rv1991c mazF6 Sequence-specific mRNA cleavage Toxin MazF6 Rv1991c, (MTCY39.28), len: 114 aa. MazF6, toxin, part of toxin-antitoxin (TA) operon with Rv1991A. Some similarity to P13976|PEMK_ECOLI pemk protein (133 aa), FASTA scores: opt: 113, E(): 0.043, (29.2% identity in 113 aa overlap); and P96622|YDCE protein from Bacillus subtilis (116 aa), FASTA scores: opt: 227, E(): 6.9e-09, (37.4% identity in 115 aa overlap). Also similar to Mycobacterium tuberculosis Rv2801c, and Rv0659c. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WII3 No virulence, detoxification, adaptation P64911 P9WII3 GO:0003677 P9WII3 NC_000962.3 Mycobrowser_v5 CDS 2234643 2234891 . - 0 Rv1991A mazE6 Unknown Antitoxin MazE6 Rv1991A, len: 82 aa. MazE6, antitoxin, part of toxin-antitoxin (TA) operon with Rv1991c. Similar to ChpI of L. interrogans, FASTA scores: opt: 134, E(): 0.024, 29.762% identity (65.476% similar) in 84 aa overlap. Note that Pandey and Gerdes, 2005 predicts a different N-terminus, adding 10 amino acids. P9WJ87 No virulence, detoxification, adaptation P9WJ87 NC_000962.3 Mycobrowser_v5 CDS 2237303 2237575 . - 0 Rv1993c Rv1993c Function unknown Conserved protein Rv1993c, (MTCY39.26), len: 90 aa. Conserved protein, very similar to Rv3269|Z92771|MTCY71.09 hypothetical protein from Mycobacterium tuberculosis (93 aa), FASTA results: opt: 309, E(): 3.2e-16, (63.3% identity in 79 aa overlap). Also similar to Rv0968 (98 aa) (51.1% identity in 94 aa overlap). P9WLP5 No conserved hypotheticals P64913 P9WLP5 NC_000962.3 Mycobrowser_v5 CDS 2237628 2237984 . - 0 Rv1994c cmtR Represses transcription from the CMT operator-promoter. Repression is alleviated by CD(II). Metal sensor transcriptional regulator CmtR (ArsR-SmtB family) Rv1994c, (MTCY39.25), len: 118 aa. CmtR, transcriptional regulator (See Cavet et al., 2003). Similar to MERR_STRLI|P30346 probable mercury resistance operon repressor (125 aa), FASTA scores: opt: 199, E(): 3e-08, (36.3% identity in 102 aa overlap). Note that primer extension analysis revealed two transcriptional start sites (See Chauhan et al., 2009). P9WMI9 No regulatory proteins 2JSC P67731 P9WMI9 GO:0046872,GO:0006355,GO:0006350,GO:0003700,GO:0005622 P9WMI9 NC_000962.3 Mycobrowser_v5 promoter 2238001 2238036 . - 0 Rv1994c cmtR Represses transcription from the CMT operator-promoter. Repression is alleviated by CD(II). Metal sensor transcriptional regulator CmtR (ArsR-SmtB family) Rv1994c, (MTCY39.25), len: 118 aa. CmtR, transcriptional regulator (See Cavet et al., 2003). Similar to MERR_STRLI|P30346 probable mercury resistance operon repressor (125 aa), FASTA scores: opt: 199, E(): 3e-08, (36.3% identity in 102 aa overlap). Note that primer extension analysis revealed two transcriptional start sites (See Chauhan et al., 2009). P9WMI9 No regulatory proteins 2JSC P67731 P9WMI9 GO:0046872,GO:0006355,GO:0006350,GO:0003700,GO:0005622 P9WMI9 NC_000962.3 Mycobrowser_v5 CDS 2239004 2239957 . + 0 Rv1996 Rv1996 Function unknown Universal stress protein family protein Rv1996, (MTCY39.23c), len: 317 aa. Universal stress protein family protein. Similar to several Mycobacterium tuberculosis hypothetical proteins e.g. Rv2005c|Q10851|YK05_MYCTU (295 aa), FASTA scores: opt: 775, E(): 0, (50.3% identity in 316 aa overlap); Rv2026c (294 aa) (47.9% identity in 311 aa overlap); and Rv2623, etc. Also similar to SCJ1.30c|AL109962 hypothetical protein from Streptomyces coelicolor (328 aa). Predicted possible vaccine candidate (See Zvi et al., 2008). P9WLP1 No virulence, detoxification, adaptation P0A5F7 P9WLP1 GO:0006950 P9WLP1 NC_000962.3 Mycobrowser_v5 CDS 2240159 2242876 . + 0 Rv1997 ctpF Metal cation-transporting ATPase; possibly catalyzes the transport of an undetermined metal cation with the hydrolysis of ATP [catalytic activity: ATP + H(2)O + undetermined metal cation(in) = ADP + phosphate + undetermined metal cation(out)]. Probable metal cation transporter P-type ATPase A CtpF Rv1997, (MTCY39.22c, MTCY39.21c), len: 905 aa. Probable ctpF, metal cation-transporting P-type ATPase F (transmembrane protein), highly similar to others e.g. NP_250120.1|NC_002516 probable cation-transporting P-type ATPase from Pseudomonas aeruginosa (902 aa); NP_441217.1|NC_000911 cation-transporting ATPase (E1-E2 ATPase) from Synechocystis sp. strain PCC 6803 (905 aa); NP_404093.1|NC_003143 putative cation-transporting P-type ATPase from Yersinia pestis (908 aa); P37367|ATA1_SYNY3 cation-transporting ATPase pma1 from Synechocystis sp. (915 aa), FASTA scores: opt: 2392, E(): 0, (46.5% identity in 852 aa overlap); etc. Contains PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases), subfamily IB. Was frame-shifted in original cosmid sequence. P9WPS9 No cell wall and cell processes P63687 P9WPS9 GO:0006754,GO:0015662,GO:0006812,GO:0016021,GO:0046872,GO:0005886,GO:0005524 3.6.3.- P9WPS9 NC_000962.3 Mycobrowser_v5 CDS 2242945 2243721 . - 0 Rv1998c Rv1998c Function unknown Conserved protein Rv1998c, (MTCY39.20), len: 258 aa. Conserved protein, showing some similarity with other hypothetical proteins e.g. U82823|SEU82823.03 Saccharopolyspora erythraea (266 aa), FASTA results: opt: 654, E(): 0, (43.8% identity in 249 aa overlap); and AL034446|SC1A9.07 Streptomyces coelicolor (251 aa), FASTA scores: opt: 592, E(): 1.5e-31, (43.4% identity in 251 aa overlap). P9WLN9 No conserved hypotheticals P9WLN9 GO:0003824 P9WLN9 NC_000962.3 Mycobrowser_v5 CDS 2256617 2257942 . - 0 Rv2008c Rv2008c Unknown Conserved hypothetical protein Rv2008c, (MTCY39.09), len: 441 aa. Conserved hypothetical protein. Contains PS00017 ATP/GTP-binding site motif A, PS00501 Signal peptidases I serine active site. Also contains helix-turn-helix motif at aa 258-279. P9WLM9 No conserved hypotheticals P64923 P9WLM9 GO:0005524 P9WLM9 NC_000962.3 Mycobrowser_v5 CDS 2261816 2263072 . - 0 Rv2015c Rv2015c Unknown Conserved hypothetical protein Rv2015c, (MTV018.02c), len: 418 aa. Conserved hypothetical protein. Nearly identical to Mycobacterium tuberculosis Rv1765c|MTCY28.31c, (378 aa), an ORF starting next to ISB9, and ending in IS6110. Different N-terminus chosen and C-terminus differs as that of Rv1765c has been truncated by IS6110. Does not show similarities with transposases. Contains IPR002711 HNH endonuclease, IPR003615 HNH nuclease, IPR003870 DUF222 domains. O53461 No conserved hypotheticals O53461 O53461 GO:0003676,GO:0004519 NC_000962.3 Mycobrowser_v5 CDS 2265280 2265999 . + 0 Rv2018 Rv2018 Unknown Conserved protein Rv2018, (MTV018.05), len: 239 aa. Conserved protein. Contains probable helix-turn-helix motif at aa 215-236 (Score 1175, +3.19 SD). O53464 No conserved hypotheticals O53464 O53464 O53464 NC_000962.3 Mycobrowser_v5 CDS 2265989 2266405 . + 0 Rv2019 Rv2019 Unknown Conserved protein Rv2019, (MTV018.06), len: 138 aa. Conserved protein. O53465 No conserved hypotheticals O53465 NC_000962.3 Mycobrowser_v5 CDS 2266421 2266720 . - 0 Rv2020c Rv2020c Unknown Conserved hypothetical protein Rv2020c, (MTV018.07c), len: 99 aa. Conserved hypothetical protein, nearly identical to C-terminal part of hypothetical protein RvD1-Rv2024c' from Mycobacterium bovis BCG (1606 aa) emb|CAB44655.1| (Y18605). Corresponds to deletion region RvD1 so probably truncated protein. O53466 No conserved hypotheticals O53466 O53466 NC_000962.3 Mycobrowser_v5 CDS 2266805 2267110 . - 0 Rv2021c Rv2021c Thought to be involved in transcriptional mechanism. Transcriptional regulatory protein Rv2021c, (MTV018.08c), len: 101 aa. Regulatory protein, similar to many. Contains probable helix-turn-helix at aa 45-66 (Score 1472, +4.20 SD); IPR001387 Helix-turn-helix type 3 domain. O53467 No regulatory proteins O53467 O53467 GO:0043565 NC_000962.3 Mycobrowser_v5 CDS 2321451 2322542 . + 0 Rv2064 cobG Required for cobalamin biosynthesis. Precorrin-3B synthase CobG Rv2064, (MTCY49.03), len: 363 aa. CobG, precorrin-3B synthase, cobalamin biosynthesis protein. Q10675 No intermediary metabolism and respiration Q10675 Q10675 GO:0016491,GO:0055114 1.14.13.83 Q10675 NC_000962.3 Mycobrowser_v5 CDS 2268268 2268726 . - 0 Rv2023A Rv2023A Unknown Hypothetical protein, pseudogene Rv2023A, len: 152 aa. Hypothetical unknown protein (pseudogene), equivalent to the C-terminus of Q8VJS0|MT2080 hypothetical protein from Mycobacterium tuberculosis strain CDC1551 (225 aa), FASTA scores: opt: 1028, E(): 3.6e-66, (99.342% identity in 152 aa overlap) and C-terminus of Mb2047c hypothetical protein from Mycobacterium bovis (225 aa). And N-terminal part equivalent to the C-terminus of Q9XB17 hypothetical 15.5 kDa protein from Mycobacterium bovis BCG (131 aa), FASTA scores: opt: 409, E(): 4.2e-22, (98.276% identity in 58 aa overlap). Note that a deletion of DNA (RvD1 region) in Mycobacterium tuberculosis strain H37Rv resulted in a truncated CDS comparatively to Mycobacterium bovis or Mycobacterium tuberculosis strain CDC1551 genomes (see citations below). Yes conserved hypotheticals NC_000962.3 Mycobrowser_v5 CDS 2270750 2271748 . - 0 Rv2025c Rv2025c Unknown; possibly involved in transport of metal ions across the membrane. Conserved membrane protein Rv2025c, (MTV018.12c), len: 332 aa. Conserved transmembrane protein, involved in transport of metal ions, contains IPR002524 Cation efflux protein domain. P9WGF5 No cell wall and cell processes O53471 P9WGF5 GO:0006812,GO:0016020,GO:0055085,GO:0008324 P9WGF5 NC_000962.3 Mycobrowser_v5 CDS 2271863 2272747 . - 0 Rv2026c Rv2026c Unknown Universal stress protein family protein Rv2026c, (MTV018.13c), len: 294 aa. Universal stress protein family protein, contains IPR006016 UspA domain. P9WFD1 No virulence, detoxification, adaptation O53472 P9WFD1 GO:0006950 P9WFD1 NC_000962.3 Mycobrowser_v5 CDS 2275405 2276424 . - 0 Rv2029c pfkB Involved in glycolysis: converts sugar-1-P to sugar-1,6-P [catalytic activity: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate]. 6-phosphofructokinase PfkB (phosphohexokinase) (phosphofructokinase) Rv2029c, (MTV018.16c), len: 339 aa. PfkB, phosphofructokinase. Contains PS00583 pfkB family of carbohydrate kinases signature 1. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WID3 No intermediary metabolism and respiration O86352 P9WID3 GO:0016301,GO:0016773,GO:0005975 2.7.1.- P9WID3 NC_000962.3 Mycobrowser_v5 CDS 2276441 2278486 . - 0 Rv2030c Rv2030c Unknown Conserved protein Rv2030c, (MTV018.17c), len: 681 aa. Conserved protein. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WLM1 No conserved hypotheticals O53475 P9WLM1 GO:0046677,GO:0016740,GO:0009116 P9WLM1 NC_000962.3 Mycobrowser_v5 CDS 2278498 2278932 . - 0 Rv2031c hspX Stress protein induced by anoxia. Has a proposed role in maintenance of long-term viability during latent, asymptomatic infections, and a proposed role in replication during initial infection. Regulated by the two component regulatory system DEVR|Rv3133c/DEVS|Rv3132c, in response to a hypoxic signal. Heat shock protein HspX (alpha-crystallin homolog) (14 kDa antigen) (HSP16.3) Rv2031c, (MTV018.18c), len: 144 aa. HspX, heat shock protein localized in the inner membrane (see citations below). Identical to P30223|14KD_MYCTU 14 KD antigen (16 kDa antigen) (HSP 16.3) of Mycobacterium tuberculosis (143 aa). Belongs to the small heat shock protein (HSP20) family. Also known as alpha-crystallin and gene as acr (see some citations below). Predicted possible vaccine candidate (See Zvi et al., 2008). P9WMK1 No virulence, detoxification, adaptation P0A5B7 P9WMK1 GO:0005737,GO:0005576,GO:0005618 P9WMK1 NC_000962.3 Mycobrowser_v5 CDS 2281294 2281617 . + 0 Rv2034 Rv2034 Involved in transcriptional regulation. ArsR repressor protein Rv2034, (MTV018.21), len: 107 aa. Repressor protein belonging to the ArsR family. Contains probable helix-turn-helix at aa 32-53 (S core 1350, +3.78 SD). O53478 No regulatory proteins O53478 O53478 GO:0006355,GO:0006350,GO:0003700,GO:0005622 O53478 NC_000962.3 Mycobrowser_v5 CDS 2281614 2282102 . + 0 Rv2035 Rv2035 Unknown Conserved hypothetical protein Rv2035, (MTV018.22), len: 162 aa. Conserved hypothetical protein, similar to many. Contains IPR013538 Activator of Hsp90 ATPase homologue 1-like. O53479 No conserved hypotheticals O53479 O53479 GO:0006950 O53479 NC_000962.3 Mycobrowser_v5 CDS 2283723 2284796 . - 0 Rv2038c Rv2038c Thought to be involved in active transport of sugar across the membrane (import). Responsible for energy coupling to the transport system. Probable sugar-transport ATP-binding protein ABC transporter Rv2038c, (MTV018.25c), len: 357 aa. Probable sugar-transport ATP-binding protein ABC transporter (see citation below), similar to many. Contains PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). O53482 No cell wall and cell processes O53482 O53482 GO:0043190,GO:0016887,GO:0016820,GO:0006810,GO:0005524 O53482 NC_000962.3 Mycobrowser_v5 CDS 2284799 2285641 . - 0 Rv2039c Rv2039c Thought to be involved in active transport of sugar across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable sugar-transport integral membrane protein ABC transporter Rv2039c, (MTV018.26c), len: 280 aa. Probable sugar-transport integral membrane protein ABC transporter (see citation below), similar to many. Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature. Also contains possible helix-turn-helix motif at aa 171-192, although this is probably fortuitous. O53483 No cell wall and cell processes O53483 O53483 GO:0005886,GO:0006810,GO:0005215,GO:0016021 O53483 NC_000962.3 Mycobrowser_v5 CDS 2286527 2287846 . - 0 Rv2041c Rv2041c Thought to be involved in active transport of sugar across the membrane (import). Probable sugar-binding lipoprotein Rv2041c, (MTV018.28c), len: 439 aa. Probable sugar-binding lipoprotein component of sugar transport system, similar to many. Contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O53485 No cell wall and cell processes O53485 O53485 GO:0006810,GO:0005215 O53485 NC_000962.3 Mycobrowser_v5 CDS 2288681 2289241 . - 0 Rv2043c pncA Converts amides such as nicotinamide to corresponding acid. Pyrazinamidase/nicotinamidase PncA (PZase) Rv2043c, (MTV018.30c), len: 186 aa. PncA, pyrazinamidase/nicotinamidase (see citations below), involved in susceptibility or resistance to antituberculous drug pyrazinamide. I6XD65 No intermediary metabolism and respiration 3GBC,3PL1 Q50575 I6XD65 GO:0008152,GO:0016787 3.5.1.- PZA I6XD65 NC_000962.3 Mycobrowser_v5 CDS 2289685 2291220 . - 0 Rv2045c lipT Converts unknown esters to corresponding free acid and alcohol Carboxylesterase LipT Rv2045c, (MTV018.32c), len: 511 aa. LipT, carboxylesterase, similar to many. Contains PS00941 Carboxylesterases type-B signature 2. Contains PS00122 Carboxylesterases type-B serine active site. O53488 No intermediary metabolism and respiration O53488 O53488 GO:0008152,GO:0016787 3.1.1.- O53488 NC_000962.3 Mycobrowser_v5 CDS 2291962 2294526 . - 0 Rv2047c Rv2047c Unknown Conserved hypothetical protein Rv2047c, (MTV018.34c), len: 854 aa. Conserved hypothetical protein, similar to many. Contains IPR016040 NAD(P)-binding domain at N-terminus. P9WIH5 No conserved hypotheticals P65684 P9WIH5 GO:0016310,GO:0016772,GO:0050662 P9WIH5 NC_000962.3 Mycobrowser_v5 CDS 2310913 2312517 . - 0 Rv2052c Rv2052c Unknown Conserved protein Rv2052c, (MTV018.39c), len: 534 aa. Conserved protein, similar to many. Contains IPR013108 Amidohydrolase 3 domain. O53494 No conserved hypotheticals O53494 O53494 GO:0016810 O53494 NC_000962.3 Mycobrowser_v5 CDS 2312522 2313049 . - 0 Rv2053c fxsA Unknown Probable transmembrane protein FxsA Rv2053c, (MTV018.40c-MTCY63A.07), len: 175 aa. Probable fxsA, transmembrane protein. Contains IPR007313 FxsA cytoplasmic membrane protein domain in N-terminus O53495 No cell wall and cell processes O53495 O53495 GO:0016021 O53495 NC_000962.3 Mycobrowser_v5 CDS 2313125 2313838 . + 0 Rv2054 Rv2054 Unknown Conserved protein Rv2054, (MTCY63A.06c), len: 237 aa. Conserved protein, similar to many. Contains IPR002925 Dienelactone hydrolase domain. O86353 No conserved hypotheticals O86353 O86353 GO:0016787 O86353 NC_000962.3 Mycobrowser_v5 CDS 2314087 2314353 . - 0 Rv2055c rpsR2 Involved in translation, amino-acyl tRNA binding 30S ribosomal protein S18 RpsR2 Rv2055c, (MTCY63A.05), len: 88 aa. rpsR2, 30S ribosomal protein S18, similar to many. Also similar to rpsR|Rv0055|MTCY21D4.18 from Mycobacterium tuberculosis (50.0% identity in 84 aa overlap). P9WH47 No information pathways P66465 P9WH47 GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WH47 NC_000962.3 Mycobrowser_v5 CDS 2315174 2316709 . + 0 Rv2059 Rv2059 Unknown Conserved hypothetical protein Rv2059, (MTCY63A.01c), len: 511 aa. Conserved hypothetical protein. Some similarity to EWLA protein gp|U52850|ERU52850_1 Erysipelothrix rhusiopathiae 36 k (304 aa), FASTA score, opt: 287 E(): 6.9e-09; 27.2% identity in 228 aa overlap. There appears to be a frameshift in this ORF around position 3315980 that causes an overlap with next ORF. C-terminal end of protein may be wrong. No error can be found to account for this. O07257 No conserved hypotheticals O07257 O07257 GO:0030001,GO:0046872 O07257 NC_000962.3 Mycobrowser_v5 CDS 2316279 2316680 . + 0 Rv2060 Rv2060 Unknown Possible conserved integral membrane protein Rv2060, (MTV019.01), len: 133 aa. Possible conserved integral membrane protein smaller than but similar to several hypothetical bacterial proteins e.g. >emb|CAC29843.1| (AL583918) putative ABC-transporter transmembrane protein [Mycobacterium leprae] Length = 286 and P44691|YEBI_HAEIN (261 aa). FASTA scores: P44691|YEBI_HAEIN hypothetical protein HI0407 (261 aa) opt: 218, E(): 4.2e-08; 31.1% identity in 122 aa overlap. Maybe frameshift upstream at position 3315980 but no error can be found to account for this. O86339 No cell wall and cell processes O86339 O86339 GO:0042626,GO:0016021,GO:0006810,GO:0005524 O86339 NC_000962.3 Mycobrowser_v5 CDS 2320831 2321064 . + 0 Rv2063 mazE7 Function unknown Antitoxin MazE7 Rv2063, len: 77 aa. MazE7, antitoxin, part of toxin-antitoxin (TA) operon with Rv2063A (See Pandey and Gerdes, 2005), similar to many. This ORF replaces previous Rv2063c on other strand. P9WJ85 No virulence, detoxification, adaptation Q8VJR2 P9WJ85 GO:0006355,GO:0003677 NC_000962.3 Mycobrowser_v5 CDS 2324647 2325870 . - 0 Rv2067c Rv2067c Unknown Conserved protein Rv2067c, (MTCY49.06c), len: 407 aa. Conserved protein, similar to many. P9WLL9 No conserved hypotheticals P0A5G1 P9WLL9 P9WLL9 NC_000962.3 Mycobrowser_v5 CDS 2325886 2326809 . - 0 Rv2068c blaC Hydrolyses beta-lactams to generate corresponding beta-amino acid [catalytic activity: a beta-lactam + H(2)O = a substituted beta-amino acid]. Class a beta-lactamase BlaC Rv2068c, (MTCY49.07c), len: 307 aa. BlaC, class a beta-lactamase (see citation below), similar to many. Contains PS00013 Prokaryotic lipid attachment site near N-terminus, and PS00146 Beta-lactamase class-a active site. P9WKD3 No intermediary metabolism and respiration 2GDN,3CG5,3IQA,3M6B,3M6H,3DWZ,3N8S,3NY4,3N6I,3N7W,3N8L,3N8R,3NBL,3NC8,3NCK,3NDE,3NDG P0C5C1 P9WKD3 GO:0008800,GO:0005886,GO:0046677,GO:0030655 3.5.2.6 P9WKD3 NC_000962.3 Mycobrowser_v5 CDS 2328222 2328977 . - 0 Rv2071c cobM Involved in cobalamin biosynthesis. Precorrin-3 methylase CobM (precorrin-4 C11-methyltransferase) Rv2071c, (MTCY49.10c), len: 251 aa. CobM, precorrin-3 methylase, similar to many. Contains PS00839 Uroporphyrin-III C-methyltransferase signature 1, and PS00840 Uroporphyrin-III C-methyltransferase signature 2. P9WGB1 No intermediary metabolism and respiration Q10672 P9WGB1 GO:0046026,GO:0009236 2.1.1.133 P9WGB1 NC_000962.3 Mycobrowser_v5 CDS 2328974 2330146 . - 0 Rv2072c cobL Involved in cobalamin biosynthesis. Precorrin-6Y C(5,15)-methyltransferase (decarboxylating) CobL Rv2072c, (MTCY49.11c), len: 390 aa. CobL, precorrin-6Y C(5,15)-methyltransferase (decarboxylating). P9WGA9 No intermediary metabolism and respiration Q10671 P9WGA9 GO:0046025,GO:0008276,GO:0009236 2.1.1.132 P9WGA9 NC_000962.3 Mycobrowser_v5 CDS 2330993 2331406 . + 0 Rv2074 Rv2074 May be involved in biosynthesis of pyridoxine (vitamin B6) and pyridoxal phosphate. Oxidize PNP and PMP into pyridoxal 5'-phosphate (PLP) [catalytic activity: pyridoxamine 5'-phosphate + H2O + O2 = pyridoxal 5'-phosphate + NH3 + H2O2] Possible pyridoxamine 5'-phosphate oxidase (PNP/PMP oxidase) (pyridoxinephosphate oxidase) (PNPOX) (pyridoxine 5'-phosphate oxidase) Rv2074, (MTCY49.13), len: 137 aa. Possible pyridoxine 5'-phosphate oxidase (PNPOx) (See Biswal et al., 2006). Similar to conserved hypothetical proteins. P9WLL7 No intermediary metabolism and respiration 2ASF Q10682 P9WLL7 GO:0010181 P9WLL7 NC_000962.3 Mycobrowser_v5 CDS 2331416 2332879 . - 0 Rv2075c Rv2075c Unknown Possible hypothetical exported or envelope protein Rv2075c, (MTCY49.14c), len: 487 aa. Possibly exported or envelope protein; has potential signal peptide at N-terminus and hydrophobic stretch around residue 430. Predicted to be an outer membrane protein (See Song et al., 2008). P9WLL5 No cell wall and cell processes P9WLL5 GO:0006629,GO:0004629,GO:0005886,GO:0016021 P9WLL5 NC_000962.3 Mycobrowser_v5 CDS 2333323 2334294 . - 0 Rv2077c Rv2077c Unknown Possible conserved transmembrane protein Rv2077c, (MTCY49.16c), len: 323 aa. Possible conserved transmembrane protein. Part of Mycobacterium tuberculosis protein family with Rv2542, Rv2079, Rv2797c, Rv0963c, Rv1949c. Hydrophobic stretches at C-terminus. P9WLL1 No cell wall and cell processes P9WLL1 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2334295 2334594 . - 0 Rv2077A Rv2077A Unknown Conserved hypothetical protein Rv2077A, len: 99 aa. Conserved hypothetical protein, similar to many. L7N6B8 No conserved hypotheticals L7N6B8 NC_000962.3 Mycobrowser_v5 CDS 2335059 2335373 . + 0 Rv2078 Rv2078 Unknown Conserved hypothetical protein Rv2078, (MTCY49.17), len: 104 aa. Conserved hypothetical protein, similar to many. P9WLK9 No conserved hypotheticals Q10686 P9WLK9 NC_000962.3 Mycobrowser_v5 CDS 2343027 2343332 . + 0 Rv2085 Rv2085 Unknown Conserved hypothetical protein Rv2085, (MTCY49.24), len: 101 aa. Conserved hypothetical protein, similar to but shorter than many transposases but we can find no sequence errors to account for the frameshifts. Contains possible helix-turn-helix motif at aa 33 to 54,(+3.11 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLJ9 No insertion seqs and phages P9WLJ9 NC_000962.3 Mycobrowser_v5 CDS 2338709 2340874 . + 0 Rv2082 Rv2082 Unknown Conserved hypothetical protein Rv2082, (MTCY49.21), len: 721 aa. Conserved hypothetical protein. Similar to Mycobacterium tuberculosis Rv0029, and to Rv3899c and Rv3900c which may be frameshifted. Q10690 No conserved hypotheticals Q10690 Q10690 NC_000962.3 Mycobrowser_v5 CDS 2343311 2343916 . + 0 Rv2086 Rv2086 Unknown Conserved hypothetical protein Rv2086, (MTCY49.25), len: 201 aa. Conserved hypothetical protein, similarity to but shorter than many transposases but we can find no sequence errors to account for the frameshifts. Start changed since first submission (-16 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P64937 No insertion seqs and phages P64937 P64937 NC_000962.3 Mycobrowser_v5 CDS 2343994 2344224 . + 0 Rv2087 Rv2087 Unknown Conserved hypothetical protein Rv2087, (MTCY49.27), len: 76 aa. Conserved hypothetical protein, similar to but shorter than transposases, but we can find no sequence errors to account for the frameshifts. Start changed since first submission (-45 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLJ7 No insertion seqs and phages P9WLJ7 NC_000962.3 Mycobrowser_v5 CDS 2346197 2347324 . - 0 Rv2089c pepE Hydrolysis of peptide bonds Dipeptidase PepE Rv2089c, (MTCY49.29c), len: 375 aa. PepE, dipeptidase, similar to many; contains PS00491 Aminopeptidase P and proline dipeptidase signature. Also similar to Mycobacterium tuberculosis peptidases Rv2861c, Rv0734, Rv2535c. Phosphorylated in vitro by PknJ|Rv2088 (See Jang et al., 2010). P9WHS7 No intermediary metabolism and respiration P65810 P9WHS7 GO:0006508,GO:0016021,GO:0046872,GO:0008235,GO:0005886,GO:0009987 3.4.13.- P9WHS7 NC_000962.3 Mycobrowser_v5 CDS 2347373 2348554 . + 0 Rv2090 Rv2090 DNA metabolism Probable 5'-3' exonuclease Rv2090, (MTCY49.30), len: 393 aa. Probable 5'-3' exonuclease, similar to exonuclease part of DNA polymerase. Belongs to family a of DNA polymerases. P9WNU3 No information pathways Q10699 P9WNU3 GO:0003677,GO:0006281,GO:0008409 3.1.11.- P9WNU3 NC_000962.3 Mycobrowser_v5 CDS 2369726 2370601 . - 0 Rv2110c prcB Protein degradation Proteasome beta subunit PrcB; assembles with alpha subunit PrcA. Rv2110c, (MTCY261.06c), len: 291 aa. PrcB, proteasome beta-type subunit 2. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). prcBA genes encode a proteasome with broad substrate specificity (See Lin et al., 2006). P9WHT9 No intermediary metabolism and respiration 2FHG,2FHH,2JAY,3H6F,3H6I,3HF9,3HFA,3MKA,3KRD,3MFE,3MI0 O33245 P9WHT9 GO:0009405,GO:0005839,GO:0051603,GO:0004298,GO:0005737 3.4.25.1 P9WHT9 NC_000962.3 Mycobrowser_v5 CDS 2353046 2353297 . - 0 Rv2094c tatA Involved in protein export: required for correct localization of precursor proteins bearing signal peptides with the twin arginine conserved motif S/T-R-R-X-F-L-K. This sec-independent pathway is termed tat for twin-arginine translocation system. This system mainly transports proteins with bound cofactors that require folding prior to export. Sec-independent protein translocase membrane-bound protein TatA Rv2094c, (MT2155, MTCY49.34c), len: 83 aa. TatA, membrane-bound protein, component of twin-arginine translocation protein export system (see Berks et al., 2000), similar to many. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the TATA/E family. P9WGA1 No cell wall and cell processes P66889 P9WGA1 GO:0005886,GO:0009306,GO:0008565,GO:0055085,GO:0016021 P9WGA1 NC_000962.3 Mycobrowser_v5 CDS 2356729 2358033 . - 0 Rv2098c PE_PGRS36 Function unknown PE-PGRS family protein PE_PGRS36 Rv2098c, (MTCY49.38c), len: 434 aa. PE_PGRS36, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below). Frameshifted near N-terminus (see Rv2099c|PE21). P0A688 Yes PE/PPE P0A688 P0A688 NC_000962.3 Mycobrowser_v5 CDS 2352103 2353029 . - 0 Rv2093c tatC Involved in proteins export: required for correct localization of precursor proteins bearing signal peptides with the twin arginine conserved motif S/T-R-R-X-F-L-K. This sec-independent pathway is termed tat for twin-arginine translocation system. This system mainly transports proteins with bound cofactors that require folding prior to export. Sec-independent protein translocase transmembrane protein TatC Rv2093c, (MT2154, MTCY49.33c), len: 308 aa. TatC, transmembrane protein, component of twin-arginine translocation protein export system (see citation below), similar to many. Belongs to the TatC family. P9WG97 No cell wall and cell processes P66895 P9WG97 GO:0005886,GO:0015031,GO:0055085,GO:0016021 P9WG97 NC_000962.3 Mycobrowser_v5 CDS 2353365 2354315 . - 0 Rv2095c pafC Unknown Proteasome accessory factor C PafC Rv2095c, (MTCY49.35c), len: 316 aa. PafC, proteasome accessory factor C, similar to many. Contains possible helix-turn-helix motif at aa 25-46, (+2.92 SD). PafB|Rv2096c and PafC|Rv2095c interact (See Festa et al., 2007). P9WIL9 No intermediary metabolism and respiration P9WIL9 P9WIL9 NC_000962.3 Mycobrowser_v5 CDS 2354312 2355310 . - 0 Rv2096c pafB Unknown Proteasome accessory factor B PafB Rv2096c, (MTCY49.36c), len: 332 aa. PafB, proteasome accessory factor B, similar to many. PafB|Rv2096c and PafC|Rv2095c interact (See Festa et al., 2007). P9WIM1 No intermediary metabolism and respiration P9WIM1 P9WIM1 NC_000962.3 Mycobrowser_v5 CDS 2355319 2356677 . - 0 Rv2097c pafA Ligates deamidated pup to proteasomal substrate proteins. Requires hydrolysis of ATP to ADP. Proteasome accessory factor a PafA Rv2097c, (MTCY49.37c), len: 452 aa. PafA, proteasome accessory factor A, similar to many. Belongs to the carboxylate amine/ammonia ligase family. P9WNU7 No intermediary metabolism and respiration P64943 P9WNU7 GO:0016881,GO:0019941,GO:0009405,GO:0010498,GO:0005524 6.3.2.n2 P9WNU7 NC_000962.3 Mycobrowser_v5 CDS 2370905 2372569 . - 0 Rv2112c dop Deamidates the C-terminal glutamine of pup Deamidase of pup Dop Rv2112c, (MTCY261.08c), len: 554 aa. Dop, deamidase of Pup (See Streibel et al., 2009). Highly similar to many. Cofactor: ATP. Predicted to be an outer membrane protein (See Song et al., 2008). P9WNU9 No intermediary metabolism and respiration O33247 P9WNU9 GO:0019941,GO:0010498,GO:0016787 3.5.1.- P9WNU9 NC_000962.3 Mycobrowser_v5 CDS 2363391 2364107 . + 0 Rv2102 Rv2102 Unknown Conserved hypothetical protein Rv2102, (MTV020.02), len: 238 aa. Conserved hypothetical protein, similar to many. Contains PS00017 ATP/GTP-binding site motif A (P-loop). O53500 No conserved hypotheticals O53500 O53500 GO:0008270 NC_000962.3 Mycobrowser_v5 CDS 2364086 2364520 . - 0 Rv2103c vapC37 Unknown Possible toxin VapC37. Contains PIN domain. Rv2103c, (MTV020.03), len: 144 aa. Possible vapC37, toxin, part of toxin-antitoxin (TA) operon with Rv2104c, contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis including Rv0749, Rv0277c, Rv2530c, Rv3320c, Rv2494, Rv2872, Rv0617, Rv1242 etc. O53501 No virulence, detoxification, adaptation O53501 O53501 O53501 NC_000962.3 Mycobrowser_v5 CDS 2364527 2364781 . - 0 Rv2104c vapB37 Unknown Possible antitoxin VapB37 Rv2104c, (MTV020.04), len: 84 aa. Possible vapB37, antitoxin, part of toxin-antitoxin (TA) operon with Rv2103c, see Arcus et al. 2005. Similar to others in M. tuberculosis including Rv2871, Rv1241, Rv2132, Rv3321c, Rv1113, Rv0657, Rv1560, etc. P9WJ27 No virulence, detoxification, adaptation P9WJ27 NC_000962.3 Mycobrowser_v5 CDS 2365465 2365791 . + 0 Rv2105 Rv2105 Required for the transposition of the insertion element IS6110. Putative transposase for insertion sequence element IS6110 (fragment) Rv2105, (MTCY261.01), len: 108 aa. Putative transposase for IS6110 (fragment), identical to many other Mycobacterium tuberculosis IS6110 transposase subunits e.g. Q50686|YIA4_MYCTU Insertion element IS6110 hypothetical 12.0 kDa protein (108 aa), fasta scores: E(): 1.4e-43, (100.00% identity in 108 aa overlap). The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2105 and Rv2106, the sequence UUUUAAAG (directly upstream of Rv2106) maybe responsible for such a frameshifting event (see McAdam et al., 1990). P9WKH5 No insertion seqs and phages P0C5G9 P9WKH5 GO:0004803,GO:0006313,GO:0003677 P9WKH5 NC_000962.3 Mycobrowser_v5 CDS 2365740 2366726 . + 0 Rv2106 Rv2106 Required for the transposition of the insertion element IS6110. Probable transposase Rv2106, (MTCY261.02), len: 328 aa. Probable transposase subunit for IS6110. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2105 and Rv2106, the sequence UUUUAAAG (directly upstream of Rv2106) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 16 aa). P9WKH9 No insertion seqs and phages P0C5G8 P9WKH9 GO:0015074,GO:0006310,GO:0032196,GO:0003677 P9WKH9 NC_000962.3 Mycobrowser_v5 CDS 2367711 2368442 . + 0 Rv2108 PPE36 Function unknown PPE family protein PPE36 Rv2108, (MTCY261.04), len: 243 aa. PPE36, Member of the Mycobacterium tuberculosis PE family: N-terminus is similar to N-terminal region of Mycobacterium tuberculosis PPE family proteins. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WI01 No PE/PPE P95315 P9WI01 GO:0005886 P9WI01 NC_000962.3 Mycobrowser_v5 CDS 2368983 2369729 . - 0 Rv2109c prcA Protein degradation Proteasome alpha subunit PrcA; assembles with beta subunit PrcB. Rv2109c, (MTCY261.05c), len: 248 aa. PrcA, proteasome alpha-type subunit 1. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). prcBA genes encode a proteasome with broad substrate specificity (See Lin et al., 2006). P9WHU1 No intermediary metabolism and respiration 2FHG,2FHH,3H6F,3H6I,3HF9,3HFA,3MKA,3KRD,3MFE,3MI0 O33244 P9WHU1 GO:0009405,GO:0019773,GO:0051603,GO:0004298,GO:0005737 3.4.25.1 P9WHU1 NC_000962.3 Mycobrowser_v5 CDS 2373834 2374457 . + 0 Rv2114 Rv2114 Unknown Conserved protein Rv2114, (MTCY261.10), len: 207 aa. Conserved protein, similar to many. O33249 No conserved hypotheticals O33249 NC_000962.3 Mycobrowser_v5 CDS 2376571 2377140 . + 0 Rv2116 lppK Unknown Conserved lipoprotein LppK Rv2116, (MTCY261.12), len: 189 aa. LppK, conserved lipoprotein, similar to many. Contains N-terminal signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Some similarity to Rv2376c. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WK75 No cell wall and cell processes P9WK75 GO:0005886 P9WK75 NC_000962.3 Mycobrowser_v5 CDS 2377148 2377441 . + 0 Rv2117 Rv2117 Unknown Conserved hypothetical protein Rv2117, (MTCY261.13), len: 97 aa. Conserved hypothetical protein, similar to many. O33252 No conserved hypotheticals O33252 O33252 NC_000962.3 Mycobrowser_v5 CDS 2377470 2378312 . - 0 Rv2118c Rv2118c Involved in transfer of methyl group (from S-adenosyl-L-methionine to the substrate). RNA methyltransferase Rv2118c, (MTCY261.14c), len: 280 aa. S-adenosyl-l-methionine-dependent RNA methyltransferase (see citation below), similar to many. The larger catalytic C-terminal domain binds the cofactor S-adenosyl-l-methionine (AdoMet) and is involved in the transfer of methyl group from AdoMet to the substrate. P9WFZ1 No intermediary metabolism and respiration 1I9G O33253 P9WFZ1 GO:0030488,GO:0016429 2.1.1.- P9WFZ1 NC_000962.3 Mycobrowser_v5 CDS 2378386 2379222 . + 0 Rv2119 Rv2119 Unknown Conserved hypothetical protein Rv2119, (MTCY261.15), len: 278 aa. Conserved hypothetical protein, similar to many. O33254 No conserved hypotheticals O33254 O33254 O33254 NC_000962.3 Mycobrowser_v5 CDS 2379245 2379727 . - 0 Rv2120c Rv2120c Unknown Probable conserved integral membrane protein Rv2120c, (MTCY261.16c), len: 160 aa. Probable conserved integral membrane protein, similar to many. O33255 No cell wall and cell processes O33255 NC_000962.3 Mycobrowser_v5 CDS 2379806 2380660 . - 0 Rv2121c hisG Involved in histidine biosynthesis. ATP phosphoribosyltransferase HisG Rv2121c, (MTCY261.17c), len: 284 aa. HisG, ATP phosphoribosyltransferase (see citation below), similar to many. P9WMN1 No intermediary metabolism and respiration 1NH7,1NH8 P60759 P9WMN1 GO:0003879,GO:0005737,GO:0000105,GO:0000287,GO:0005524 2.4.2.17 P9WMN1 NC_000962.3 Mycobrowser_v5 CDS 2380663 2380944 . - 0 Rv2122c hisE Involved in histidine biosynthesis. Phosphoribosyl-AMP pyrophosphatase HisE Rv2122c, (MTCY261.18), len: 93 aa. HisE (alternate gene name: irg1), phosphoribosyl-AMP cyclohydrolase (see citation below), similar to many. Note that previously misnamed hisI. P9WMM9 No intermediary metabolism and respiration 1Y6X,3C90 P0A5B1 P9WMM9 GO:0005737,GO:0000105,GO:0004636,GO:0005524 3.6.1.31 P9WMM9 NC_000962.3 Mycobrowser_v5 CDS 2381071 2382492 . + 0 Rv2123 PPE37 Function unknown PPE family protein PPE37 Rv2123, (MTCY261.19), len: 473 aa. PPE37 (alternate gene name: irg2), member of the Mycobacterium tuberculosis PPE family of proteins but the C-terminus is not repetitive (see citation below). Q79FH3 No PE/PPE Q79FH3 Q79FH3 Q79FH3 NC_000962.3 Mycobrowser_v5 CDS 2405666 2405956 . - 0 Rv2146c Rv2146c Unknown Possible conserved transmembrane protein Rv2146c, (MTCY270.22), len: 96 aa. Possible conserved transmembrane protein, orthologs present in M. leprae, ML0921 (96 aa) and Streptomyces coelicolor. Second start taken GTG alternative upstream but much less probable in TBParse. FASTA best: Q44935 similar to a hypothetical integral membrane prot EIN (97 aa) opt: 105, E(): 0.093; (25.3% identity in 87 aa overlap). >emb|CAC31302.1| (AL583920) possible membrane protein ML0921 [Mycobacterium leprae] E(): 5e-32 (76% identity in 96 aa overlap) O06230 No cell wall and cell processes O06230 O06230 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2390308 2391189 . - 0 Rv2129c Rv2129c Unknown Probable oxidoreductase Rv2129c, (MTCY261.28), len: 293 aa. Probable oxidoreductase, similar to many e.g. FABG_SYNY3|P73826 3-oxoacyl-[acyl-carrier protein] reductase (240 aa), FASTA scores: opt: 241, E(): 5.1e-17, (32.7% identity in 196 aa overlap); etc. Also similar to a number of other Mycobacterium tuberculosis oxidoreductases e.g. MTCY210.04 (34.1% identity in 217 aa overlap). O33263 No intermediary metabolism and respiration O33263 O33263 GO:0055114,GO:0016491 1.-.-.- O33263 NC_000962.3 Mycobrowser_v5 CDS 2391215 2392459 . - 0 Rv2130c mshC Involved in the third step of mycothiol biosynthesis Cysteine:1D-myo-inosityl 2-amino-2-deoxy--D-glucopyranoside ligase MshC Rv2130c, (MTCY261.29c), len: 414 aa. MshC, cysteine:1D-myo-inosityl 2-amino-2-deoxy--D-glucopyranoside ligase (see Rawat et al., 2002), similar to several cysteinyl-tRNA synthetases e.g. SYC_ECOLI|P21888 cysteinyl-tRNA synthetase from Escherichia coli (461 aa), FASTA scores: opt: 535, E(): 0, (37.0% identity in 370 aa overlap); etc. Also similar to Mycobacterium tuberculosis cysS|Rv3580c|MTCY06G11.27c, (35.8% identity in 372 aa overlap). Contains a match to Pfam entry PF01406 tRNA synthetases class I (C). Previously known as cysS2. P9WJM9 No intermediary metabolism and respiration P67017 P9WJM9 GO:0004817,GO:0006423,GO:0005737,GO:0005524 6.1.1.16 P9WJM9 NC_000962.3 Mycobrowser_v5 CDS 2393851 2394639 . - 0 Rv2133c Rv2133c Unknown Conserved hypothetical protein Rv2133c, (MTCY270.35), len: 262 aa. Conserved hypothetical protein. Function: unknown but equivalent to hypothetical Mycobacterium leprae protein, Q49774. FASTA best: Q49774 B2126_C1_150 (262 aa) opt: 1447, E(): 0; (79.0% identity in 262 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06242 No conserved hypotheticals O06242 O06242 GO:0016773 O06242 NC_000962.3 Mycobrowser_v5 CDS 2395301 2396011 . - 0 Rv2135c Rv2135c Unknown Conserved protein Rv2135c, (MTCY270.33), len: 236 aa. Conserved protein. Function: unknown but equivalent to hypothetical Mycobacterium leprae protein, Q49773. FASTA best: Q49773 B2126_C1_148 opt: 1183, E() : 0; (74.8% identity in 250 aa overlap), also similar in C-terminus to PMG2_ECOLI P36942 probable phosphoglycerate mutase 2 (215 aa), FASTA scores; opt: 212, E(): 2.5e-07 27.9% identity in 190 aa overlap; and to Rv2228 and Rv2419c O06240 No conserved hypotheticals O06240 O06240 O06240 NC_000962.3 Mycobrowser_v5 tRNA 2401987 2402072 . + 0 MTB000025 leuU - tRNA-Leu (GAG) leuU, tRNA-Leu, anticodon gag, length = 86 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 2396902 2397315 . - 0 Rv2137c Rv2137c Unknown Conserved hypothetical protein Rv2137c, (MTCY270.31), len: 137 aa. Conserved hypothetical protein. C-terminus is very similar to hypothetical Mycobacterium leprae protein B2126_C2_188 (150 aa). FASTA best: Q49782 B2126_C2_188. (150 aa) opt: 469, E(): 9.6e-28; (77.2% identity in 101 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06238 No conserved hypotheticals O06238 NC_000962.3 Mycobrowser_v5 CDS 2397330 2398406 . + 0 Rv2138 lppL Unknown Probable conserved lipoprotein LppL Rv2138, (MTCY270.30c), len: 358 aa. Probable lppL, conserved lipoprotein, with appropriately placed lipoprotein signature (PS00013) strongly similar to hypothetical Mycobacterium leprae protein, Q49806. FASTA best: Q49806 B2126_F3_142. (298 aa) opt: 1495, E(): 0; (75.3% identity in 300 aa overlap). O06237 No cell wall and cell processes O06237 O06237 NC_000962.3 Mycobrowser_v5 CDS 2398720 2399793 . + 0 Rv2139 pyrD Pyrimidine biosynthesis Probable dihydroorotate dehydrogenase PyrD Rv2139, (MTCY270.29c), len: 357 aa. Probable pyrD, dihydroorotate dehydrogenase ; contains dihydroorotate dehydrogenase signatures 1 and 2 (PS00911, PS00912). FASTA best: PYRD_MYCLE P46727 dihydroorotate dehydrogenase (309 aa) opt: 1653, E(): 0; (82.6% identity in 304 aa overlap) P9WHL1 No intermediary metabolism and respiration P65908 P9WHL1 GO:0006222,GO:0004158,GO:0055114,GO:0005886,GO:0006207 1.3.5.2 P9WHL1 NC_000962.3 Mycobrowser_v5 CDS 2399798 2400328 . - 0 Rv2140c TB18.6 Unknown Conserved protein TB18.6 Rv2140c, (MTCY270.28), len: 176 aa. TB18.6, conserved protein; shows good similarity to hypothetical proteins from Streptomyces coelicolor (177 aa; 58% identity) >emb|CAC32358.1| (AL583945) and to 17.1 kDa Escherichia coli protein YbhB. FASTA best: YBHB_ECOLI P12994 hypothetical 17.1 kDa protein (158 aa) opt: 465 E( ): 2e-23; (46.2% identity in 156 aa overlap). P9WFN1 No conserved hypotheticals P67226 P9WFN1 P9WFN1 NC_000962.3 Mycobrowser_v5 CDS 2402193 2402510 . - 0 Rv2142c parE2 Unknown Possible toxin ParE2 Rv2142c, (MTCY270.26), len: 105 aa. Possible parE2, toxin, part of toxin-antitoxin (TA) operon with Rv2142A (See Pandey and Gerdes, 2005). P9WHG5 No virulence, detoxification, adaptation O06233 P9WHG5 NC_000962.3 Mycobrowser_v5 CDS 2402977 2404035 . + 0 Rv2143 Rv2143 Unknown Conserved hypothetical protein Rv2143, (MTCY270.25c), len: 352 aa. Conserved hypothetical protein, strongly similar to two hypothetical mycobacterial proteins Rv2030c 2.1e-50 and Rv0571c from position 120 (Q50819; Q50111). FASTA best: Q50819 opt: 882, E() 0; (61.1% identity in 226 aa overlap). Also similar to AL021942|MTV039_9 (443 aa), FASTA scores: opt: 592, E(): 5e-30; 46.9% identity in 224 aa overlap O06232 No conserved hypotheticals O06232 O06232 GO:0016740,GO:0009116 O06232 NC_000962.3 Mycobrowser_v5 CDS 2404165 2404521 . - 0 Rv2144c Rv2144c Unknown Probable transmembrane protein Rv2144c, (MTCY270.24), len: 118 aa. Probable transmembrane protein. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06231 No cell wall and cell processes O06231 GO:0016021 O06231 NC_000962.3 Mycobrowser_v5 CDS 2431565 2431969 . - 0 Rv2169c Rv2169c Unknown Probable conserved transmembrane protein Rv2169c, (MTV021.02c), len: 134 aa. Probable conserved transmembrane protein, with orthologs in M. leprae, ML0904 probable membrane protein (134 aa), and Streptomyces coelicolor. FASTA scores with ML0904, opt: 767, E(): 5.1e-43; 86.567% identity in 134 aa overlap. emb|CAA18678.1| (AL022602) >gi|13092974|emb|CAC31285.1| (AL583920). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53503 No cell wall and cell processes O53503 O53503 GO:0016021 O53503 NC_000962.3 Mycobrowser_v5 CDS 2404616 2405398 . - 0 Rv2145c wag31 Unknown Diviva family protein Wag31 Rv2145c, (MTCY270.23), len: 260 aa. Wag31 (alternate gene name: ag84). Function unknown but corresponds to antigen 84 of Mycobacterium tuberculosis (wag31) (see Hermans et al., 1995). Predicted to contain significant amount of coiled coil structure. Some similarity to Rv1682 and Rv2927c. FASTA best: AG84_MYCTU P46816 antigen 84. Wag31|Rv2145c and PbpB|Rv2163c have been shown to interact; cleavage of PbpB|Rv2163c by Rv2869c under conditions of oxidative stress is prevented by Wag31|Rv2145c (See Mukherjee et al., 2009). P9WMU1 No cell wall and cell processes P0A5N2 P9WMU1 GO:0005737 P9WMU1 NC_000962.3 Mycobrowser_v5 CDS 2406118 2406843 . - 0 Rv2147c Rv2147c Unknown Conserved hypothetical protein Rv2147c, (MTCY270.21), len: 241 aa. Conserved hypothetical protein, similar to conserved hypothetical proteins in Mycobacterium leprae ML0920 (210 aa) and Streptomyces coelicolor. FASTA scores: >emb|CAC31301.1| (AL583920) hypothetical protein ML0920 hypothetical protein (210 aa) opt: 1242, E(): 5.7e-74; 83.486% identity in 218 aa overlap. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WGJ5 No conserved hypotheticals O06229 P9WGJ5 GO:0043093,GO:0007049,GO:0005737,GO:0005515,GO:0000917 P9WGJ5 NC_000962.3 Mycobrowser_v5 CDS 2406840 2407616 . - 0 Rv2148c Rv2148c Unknown Conserved protein Rv2148c, (MTCY270.20), len: 258 aa. Conserved protein; should belong to the YGGS/YBL036C/F09E5.8 family. FASTA best: AB003132|AB003132_5 Corynebacterium glutamicum gene (221 aa) opt: 440, E(): 2.3e-23; 42.8% identity in 236 aa overlap; and YPI1_VIBAL P52055 hypothetical protein in pilt-proc intergenic region in Vibrio alginolyticus. opt: 266, E(): 1.8e-11; 27.9% identity in 244 aa overlap. P9WFQ7 No conserved hypotheticals P67083 P9WFQ7 P9WFQ7 NC_000962.3 Mycobrowser_v5 CDS 2407622 2408374 . - 0 Rv2149c yfiH Unknown Conserved protein YfiH Rv2149c, (MTCY270.19), len: 250 aa. YfiH; corresponds to 25.3 kDa YfiH protein in ftsZ 3' region of Streptomyces griseus, and to YfiH proteins in other bacteria. Belongs to UPF0124 Family. FASTA best: YFIH_STRGR P45496, (246 aa) opt: 722, E(): 1.9e-37; (49.4% identity in 245 aa overlap) P9WKD5 No conserved hypotheticals P67256 P9WKD5 P9WKD5 NC_000962.3 Mycobrowser_v5 CDS 2408385 2409524 . - 0 Rv2150c ftsZ Essential for cell division. It is thought that the intracellular concentration of FTSZ protein is critical for productive septum formation in mycobacteria. Cell division protein FtsZ Rv2150c, (MTCY270.18), len: 379 aa. FtsZ, cell division protein (see Dziadek et al., 2002). Contains FtsZ protein signature 2 (PS01135). FASTA best: FTSZ_STRCO P45500 cell division protein FtsZ (399 aa) opt: 1674, E(): 0; (77.3% identity in 339 aa overlap). FtsW|Rv2154c interacts with PbpB|Rv2163c and FtsZ|RvRv2150c (See Datta et al., 2006). P9WN95 No cell wall and cell processes 1RLU,1RQ2,1RQ7,2Q1X,2Q1Y P64170 P9WN95 GO:0003924,GO:0000917,GO:0007049,GO:0005737,GO:0043234,GO:0051258,GO:0005525 P9WN95 NC_000962.3 Mycobrowser_v5 CDS 2442327 2443214 . - 0 Rv2180c Rv2180c Unknown Probable conserved integral membrane protein Rv2180c, (MTV021.13c), len: 295 aa. Probable conserved integral membrane protein, similar to pir||T35292 probable integral membrane protein from Streptomyces coelicolor >gi|5578858|emb|CAB51260.1| (AL096872) (246 aa) (36% identity in 249 aa overlap). O53514 No cell wall and cell processes O53514 NC_000962.3 Mycobrowser_v5 CDS 2471411 2472496 . + 0 Rv2207 cobT Involved in cobalamin biosynthesis Probable nicotinate-nucleotide-dimethylbenzimidazol phosphoribosyltransferase CobT Rv2207, (MTCY190.18), len: 361 aa. Probable cobT, phosphoribosyltransferase, similar to many e.g. SW:COBT_ECOLI P36562 nicotinate-nucleotide--dimethylbenzimidazol phosphoribosyltransferase (34.6% identity in 341 aa overlap) P9WP85 No intermediary metabolism and respiration P63841 P9WP85 GO:0015420,GO:0008939,GO:0009163,GO:0009236 2.4.2.21 P9WP85 NC_000962.3 Mycobrowser_v5 CDS 2775568 2776284 . + 0 Rv2473 Rv2473 Unknown Possible alanine and proline rich membrane protein Rv2473, (MTV008.29), len: 238 aa. Possible pro-,ala-rich membrane protein, with possible transmembrane domain around aa 81-104. O53200 No cell wall and cell processes O53200 NC_000962.3 Mycobrowser_v5 CDS 2412119 2413351 . - 0 Rv2153c murG Involved in cell wall formation; peptidoglycan biosynthesis. Probable UPD-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol-N-acetylglucosamine transferase MurG Rv2153c, (MTCY270.15), len: 410 aa. Probable murG, UPD-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol-N-acetylglucosamine transferase (see citation below), similar to others e.g. MURG_BACSU[P37585 murg protein from Bacilus subtilis (363 aa), FASTA score: opt: 494, E(): 1.1e-20, (27.9% identity in 365 aa overlap); etc. P9WJK9 No cell wall and cell processes O06224 P9WJK9 GO:0030246,GO:0007049,GO:0051301,GO:0007047,GO:0030259,GO:0009252,GO:0005886,GO:0008360,GO:0050511,GO:0019277 2.4.1.227 P9WJK9 NC_000962.3 Mycobrowser_v5 CDS 2410638 2412122 . - 0 Rv2152c murC Involved in cell wall formation; peptidoglycan biosynthesis Probable UDP-N-acetylmuramate-alanine ligase MurC Rv2152c, (MTCY270.16), len: 494 aa. Probable murC, UDP-N-acetylmuramate-alanine ligase (see citation below), similar to others e.g. MURC_ECOLI|P17952 (491 aa), FASTA scores: opt: 764, E(): 0, (36.9% identity in 474 aa overlap); etc. P9WJL7 No cell wall and cell processes P65472 P9WJL7 GO:0007049,GO:0008763,GO:0051301,GO:0007047,GO:0005737,GO:0009252,GO:0008360,GO:0005524 6.3.2.8 P9WJL7 NC_000962.3 Mycobrowser_v5 CDS 2414934 2416394 . - 0 Rv2155c murD Involved in cell wall formation; peptidoglycan biosynthesis. Probable UDP-N-acetylmuramoylalanine-D-glutamate ligase MurD Rv2155c, (MTCY270.13), len: 486 aa. Probable murD, UDP-N-acetylmuramoylalanine-D-glutamate ligase (see citation below), similar to others e.g. MURD_BACSU|Q03522 (451 aa), FASTA scores: opt: 534, E(): 2.7e-25, (28.8% identity in 483 aa overlap); etc. Contains PS01011 Folylpolyglutamate synthase signature 1. P9WJL5 No cell wall and cell processes O06222 P9WJL5 GO:0008764,GO:0007049,GO:0051301,GO:0007047,GO:0005737,GO:0009252,GO:0008360,GO:0005524 6.3.2.9 P9WJL5 NC_000962.3 Mycobrowser_v5 CDS 2416396 2417475 . - 0 Rv2156c murX Involved in cell wall formation; peptidoglycan biosynthesis. Probable phospho-N-acetylmuramoyl-pentappeptidetransferase MurX Rv2156c, (MTCY270.12), len: 359 aa. Probable murX, phospho-N-acetylmuramoyl-pentappeptidetransferase (see citation below), similar to others e.g.MRAY_ECOLI|P15876 (360 aa), FASTA scores: opt: 572, E(): 2.7e-29, (35.8% identity in 344 aa overlap); etc. P9WMW7 No cell wall and cell processes P64259 P9WMW7 GO:0051301,GO:0007047,GO:0016021,GO:0009252,GO:0008963,GO:0005886,GO:0008360,GO:0007049 2.7.8.13 P9WMW7 NC_000962.3 Mycobrowser_v5 CDS 2422271 2423137 . - 0 Rv2161c Rv2161c Unknown Conserved protein Rv2161c, (MTCY270.07), len: 288 aa. Conserved protein; shows some similarity to protein involved in lincomycin production and to other M. tuberculosis proteins e.g. Rv0953c, Rv0791c, Rv0132c, Rv2951c, Rv1855c. FASTA best: Q54379 (78-11) lincomycin production genes (295 aa) opt: 243, E(): 2.4e-09; (29.5% identity in 285 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06216 No intermediary metabolism and respiration O06216 O06216 GO:0016491,GO:0055114 O06216 NC_000962.3 Mycobrowser_v5 CDS 2533330 2533965 . + 0 Rv2260 Rv2260 Unknown Conserved hypothetical protein Rv2260, (MTV022.10), len: 211 aa. Conserved hypothetical protein, similar to hypothetical proteins Rv0634c, Rv1637c, Rv3677c, Rv2581c from Mycobacterium tuberculosis and to various hydrolases. FASTA scores: sptr|O06154|O06154 hypothetical 21.3 kDa protein (200 aa) opt: 355, E(): 4e- 15; (37.4% identity in 198 aa overlap). O53534 No conserved hypotheticals O53534 GO:0016787 O53534 NC_000962.3 Mycobrowser_v5 CDS 2947462 2947887 . - 0 Rv2620c Rv2620c Unknown Probable conserved transmembrane protein Rv2620c, (MTCY01A10.13), len: 141 aa. Probable conserved transmembrane protein, highly similar to O54184|SC7H1.25 hypothetical 14.6 KDA protein from Streptomyces coelicolor (144 aa), FASTA scores: opt: 459, E(): 1.4e-22, (56.45% identity in 140 aa overlap). Predicted possible vaccine candidate (See Zvi et al., 2008). I6Y9U6 No cell wall and cell processes I6Y9U6 GO:0016021 I6Y9U6 NC_000962.3 Mycobrowser_v5 ncRNA 2437823 2437866 . - 0 MTB000066 mcr5 Unknown Fragment of putative small regulatory RNA mcr5, fragment of putative small regulatory RNA (See DiChiara et al., 2010), cloned from M. bovis BCG Pasteur; ends not mapped, ~82 nt band detected by Northern blot. No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 2419001 2420608 . - 0 Rv2158c murE Involved in cell wall formation; peptidoglycan biosynthesis. Probable UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase MurE Rv2158c, (MTCY270.10), len: 535 aa. Probable murE, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase; UDP-N-acetylmuramyl-tripeptide synthetase (see citation below), also related to other Mycobacterium tuberculosis mur gene products. FASTA best: MURE_BACSU|Q03523 (494 aa), opt: 1020, E(): 0, (40.1% identity in 476 aa overlap). P9WJL3 No cell wall and cell processes 2WTZ,2XJA P65477 P9WJL3 GO:0008765,GO:0007049,GO:0051301,GO:0007047,GO:0005737,GO:0009252,GO:0008360,GO:0005524 6.3.2.13 P9WJL3 NC_000962.3 Mycobrowser_v5 CDS 2420631 2421665 . - 0 Rv2159c Rv2159c Unknown Conserved protein Rv2159c, (MTCY270.09), len: 344 aa. Conserved protein; some similarity to hypothetical protein from Streptomyces coelicolor SC1A6.09c (337 aa, 29% identity). Smith-Waterman scores: >pir||T28690 hypothetical protein - Streptomyces coelicolor >gi|3127841|emb|CAA18907.1| (AL023496) Expect = 2e-18. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06218 No conserved hypotheticals O06218 O06218 O06218 NC_000962.3 Mycobrowser_v5 CDS 2421643 2422278 . - 0 Rv2160A Rv2160A Unknown Conserved hypothetical protein Rv2160A, len: 211 aa. Conserved hypothetical protein, possibly a TetR-family transcriptional regulator, similar to N-terminal half of AL512667_12|Q9AD73|SCK31.01c putative TetR-family transcriptional regulator from Streptomyces coelicolor (200 aa), FASTA scores: opt: 285, E(): 1.4e-08, (51.042% identity in 96 aa overlap). Next gene, Rv2160c, is similar to C-terminal half of 2SCK31.01c suggesting possible frameshift near 2421978 but sequence of this region has been checked and is also identical in strain CDC1551. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L0TBP1 No regulatory proteins Q79FG9 L0TBP1 GO:0006350,GO:0003700,GO:0006355 L0TBP1 NC_000962.3 Mycobrowser_v5 CDS 2421662 2422003 . - 0 Rv2160c Rv2160c Unknown Conserved hypothetical protein Rv2160c, (MTCY270.08), len: 113 aa. Conserved hypothetical protein, possibly a TetR-family transcriptional regulator, similar to C-terminal half of AL512667_12|Q9AD73|SCK31.01c putative TetR-family transcriptional regulator from Streptomyces coelicolor (200 aa), while Rv2160A is similar to the N-terminal half of 2SCK31.01c. This suggests possible frameshift near 2421978 but sequence of this region has been checked and is also identical in strain CDC1551. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q79FG9 No regulatory proteins Q79FG9 Q79FG9 NC_000962.3 Mycobrowser_v5 CDS 2423240 2424838 . - 0 Rv2162c PE_PGRS38 Function unknown PE-PGRS family protein PE_PGRS38 Rv2162c, (MTCY270.06), len: 532 aa. PE_PGRS38, Member of M. tuberculosis PE_PGRS family (see citations below). FASTA score: Y03A_MYCTU Q 10637 hypothetical glycine-rich 49.6 kDa protein (603 aa) op t: 1798 z-score: 1220.0 E(): 0; (55.4% identity in 590 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L7N6A1 No PE/PPE Q7D7F8 L7N6A1 L7N6A1 NC_000962.3 Mycobrowser_v5 CDS 2431094 2431420 . - 0 Rv2168c Rv2168c Required for the transposition of the insertion element IS6110. Putative transposase for insertion sequence element IS6110 (fragment) Rv2168c, (MTV021.01c), len: 108 aa. Putative transposase for IS6110 (fragment), identical to many other Mycobacterium tuberculosis IS6110 transposase subunits e.g. Q50686|YIA4_MYCTU Insertion element IS6110 hypothetical 12.0 kDa protein (108 aa), fasta scores: E(): 1.4e-43, (100.00% identity in 108 aa overlap). The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2167c and Rv2168c, the sequence UUUUAAAG (directly upstream of Rv2167c) maybe responsible for such a frameshifting event (see McAdam et al., 1990). P9WKH5 No insertion seqs and phages P0C5G9 P9WKH5 GO:0004803,GO:0006313,GO:0003677 P9WKH5 NC_000962.3 Mycobrowser_v5 CDS 2427084 2428238 . - 0 Rv2164c Rv2164c Unknown Probable conserved proline rich membrane protein Rv2164c, (MTCY270.04), len: 384 aa. Probable pro- rich conserved membrane protein, equivalent to ML0907|AL022602 putative conserved membrane protein from Mycobacterium leprae (377 aa) (AL022602), FASTA scores: opt: 1495, E(): 1.7e-56, (62.217% identity in 397 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06213 No cell wall and cell processes O06213 NC_000962.3 Mycobrowser_v5 CDS 2428235 2429425 . - 0 Rv2165c Rv2165c Unknown Conserved protein Rv2165c, (MTCY270.03), len: 396 aa. Conserved protein; shows strong similarity to several hypothetical bacterial proteins but has extra 80 aa residues at N-terminus FASTA best: YLXA_BACSU Q07876 hypothetical 35.3 kDa protein in ftsl (311 aa) opt: 781, E(): 0; (45.6% identity in 296 aa overlap), belongs to the YABC (E.coli), YLXA (B.subtilis) family P9WJP1 No conserved hypotheticals P65429 P9WJP1 GO:0071424,GO:0070475,GO:0005737 2.1.1.- P9WJP1 NC_000962.3 Mycobrowser_v5 CDS 2429427 2429858 . - 0 Rv2166c Rv2166c Unknown Conserved protein Rv2166c, (MTCY270.02), len: 143 aa. Conserved protein; shows strong similarity to several hypothetical bacterial proteins such as YLLB_BACSU P55343. Is equivalent to Mycobacterium leprae hypothetical protein ML0905 (143 aa, 92% identity) MLCB268.11c >sp|O69561|YL66_MYCLE hypothetical 16.1 KDA protein ML0905 >gi|3080482|emb|CAA18677.1|(AL022602) >gi|13092975|emb|CAC31286.1|(AL583920). FASTA scores: ML0905|ML0905 conserved hypothetical protein (143 aa) opt: 873, E(): 3.1e-52; 92.254% identity in 142 aa overlap; YLLB_BACSU P55343 hypothetical 16.6 kDa protein (143 aa) opt: 340, E(): 3.6e-17; (35.0% identity in 143 aa overlap). Belongs to the YABB (E.coli), YLLB (B.subtilis), MG221 (M.genitalium) family P9WJN9 No conserved hypotheticals P65436 P9WJN9 P9WJN9 NC_000962.3 Mycobrowser_v5 CDS 2430159 2431145 . - 0 Rv2167c Rv2167c Required for the transposition of the insertion element IS6110. Probable transposase Rv2167c, (MTCY270.01), len: 328 aa. Probable IS6110 transposase. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2167c and Rv2168c, the sequence UUUUAAAG (directly upstream of Rv2167c) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (- 18 aa). P9WKH9 No insertion seqs and phages P0C5G8 P9WKH9 GO:0015074,GO:0006310,GO:0032196,GO:0003677 P9WKH9 NC_000962.3 Mycobrowser_v5 CDS 2445415 2445810 . - 0 Rv2183c Rv2183c Unknown Conserved protein Rv2183c, (MTV021.16c), len: 131 aa. Conserved protein, equivalent to Mycobacterium leprae hypothetical protein ML0891 (MLCB268.25c, 130 aa). FASTA scores: opt: 558, E(): 8.3e-28; 61.832% identity in 131 aa overlap >gi|13092963|emb|CAC31272.1| (AL583920) (AL022602). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53517 No conserved hypotheticals O53517 NC_000962.3 Mycobrowser_v5 CDS 2470622 2471332 . + 0 Rv2206 Rv2206 Unknown Probable conserved transmembrane protein Rv2206, (MTCY190.17), len: 236 aa. Probable conserved transmembrane protein. Equivalent to hypothetical protein ML0869 (247 aa) of Mycobacterium leprae gZ98741|MLCB22_2 (247 aa), FASTA scores: opt: 1052, (67.5% identity in 237 aa overlap). Two hydrophobic stretches in C-terminal part. Start changed since original submission (+112 aa). P9WLI5 No cell wall and cell processes P64951 P9WLI5 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2486994 2487416 . - 0 Rv2219A Rv2219A Unknown Probable conserved membrane protein Rv2219A, len: 140 aa. Probable conserved membrane protein, similar to SC3H12.05c|AL355740_5 possible integral membrane protein from Streptomyces coelicolor (155 aa), FASTA scores: opt: 327, E(): 7.5e-14, (46.6% identity in 133 aa overlap), also linked to glnA. Q79FG7 No cell wall and cell processes Q79FG7 Q79FG7 NC_000962.3 Mycobrowser_v5 CDS 2432951 2433634 . + 0 Rv2171 lppM Unknown Probable conserved lipoprotein LppM Rv2171, (MTV021.04), len: 227 aa. Probable lppM, conserved lipoprotein; contains putative signal peptide and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Has hydrophobic stretch at C-terminus and also contains PS00225 Crystallins beta and gamma 'Greek key' motif signature. Unknown but equivalent to Mycobacterium leprae lipoprotein ML0902 (239 aa). FASTA scores: opt: 1083, E(): 2.4e-56; 75.446% identity in 224 aa overlap (5-227:16-239) >emb|CAA18680.1| (AL022602) >gi|13092972|emb|CAC31283.1| (AL583920). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53505 No cell wall and cell processes O53505 O53505 O53505 NC_000962.3 Mycobrowser_v5 CDS 2433631 2434536 . - 0 Rv2172c Rv2172c Unknown Conserved protein Rv2172c, (MTV021.05c), len: 301 aa. Conserved protein, equivalent to Mycobacterium leprae conserved hypothetical protein ML0901 (304 aa). FASTA scores: opt: 1656, E(): 7.7e-98; 81.271% identity in 299 aa overlap (1-299:1-299) >emb|CAA18681.1| (AL022602) >gi|13092971|emb|CAC31282.1| (AL583920). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53506 No conserved hypotheticals O53506 O53506 NC_000962.3 Mycobrowser_v5 CDS 2434847 2435905 . + 0 Rv2173 idsA2 Involved in lipid biosynthesis. Probable geranylgeranyl pyrophosphate synthetase IdsA2 (ggppsase) (GGPP synthetase) (geranylgeranyl diphosphate synthase) Rv2173, (MTV021.06), len: 352 aa. Probable idsA2, geranylgeranyl pyrophosphate synthase, similar to many e.g. Q54193 geranylgeranyl pyrophosphate synthase from Streptomyces griseus (425 aa). Contains PS00723 and PS00444Polyprenyl synthetases signature 1 and 2. FASTA scores: sptr|Q54193|Q54193 geranylgeranyl pyrophosphate synthase (425 aa) opt: 744, E(): 0; 39.2% identity in 352 aa overlap. O53507 No lipid metabolism O53507 O53507 GO:0016740,GO:0008299 2.5.1.- O53507 NC_000962.3 Mycobrowser_v5 CDS 2435909 2437459 . + 0 Rv2174 mptA Mannosyltransferase, likely involved in the biosynthesis of lipomannan (LM) Alpha(1->6)mannosyltransferase. Possible conserved integral membrane protein. Rv2174, (MTV021.07), len: 516 aa. MptA (mannopyranosyltransferase A) (See Mishra et al., 2007). Possible conserved integral membrane protein, similar to some hypothetical mycobacterial proteins e.g. Mycobacterium leprae ML0899 probable integral-membrane protein (505 aa) and MLCL536_26 (593 aa). FASTA scores: ML0899 opt: 2715; 78.884% identity in 502 aa overlap and gp|Z99125|MLCL536_26 Mycobacterium leprae cosmid L536. (593 aa) opt: 552, E(): 7.1e-30; 31.6% identity in 513 aa overlap. Also similar to Rv1459c. Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). O53508 No cell wall and cell processes O53508 O53508 NC_000962.3 Mycobrowser_v5 CDS 2437446 2437886 . - 0 Rv2175c Rv2175c Unknown Conserved regulatory protein Rv2175c, (MTV021.08c), len: 146 aa. Conserved protein, possibly involved in regulation. Contains possible helix-turn-helix domain at aa 31-52 (Score 1042, +2.74 SD). Equivalent to Mycobacterium leprae ML0898 putative DNA-binding protein (134 aa). FASTA scores: opt: 747; 82.090% identity in 134 aa overlap (AL022602) >gi|13092969|emb|CAC31279.1| (AL583920). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53509 No regulatory proteins 2KFS O53509 O53509 NC_000962.3 Mycobrowser_v5 CDS 2447066 2447500 . - 0 Rv2185c TB16.3 Unknown Conserved protein TB16.3 Rv2185c, (MTV021.18c), len: 144 aa. TB16.3, conserved protein, similar to other hypothetical actinomycete proteins and equivalent to Mycobacterium leprae ML0889 (144 aa). Some similarity to Mycobacterium tuberculosis Rv0854, Rv0856, Rv0857, Rv0164 and other Mycobacterium leprae proteins. FASTA scores : ML0889 opt: 811; 85.417% identity in 144 aa overlap (AL022602). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53519 No conserved hypotheticals O53519 O53519 O53519 NC_000962.3 Mycobrowser_v5 CDS 2506207 2506383 . - 0 Rv2231B vapB16 Unknown Possible antitoxin VapB16 Rv2231B, len: 58 aa. Possible vapB16, antitoxin, part of toxin-antitoxin (TA) operon with Rv2231A (See Pandey and Gerdes, 2005). P0CW31 No virulence, detoxification, adaptation P0CW31 P0CW31 NC_000962.3 Mycobrowser_v5 CDS 2439282 2439947 . - 0 Rv2177c Rv2177c Required for the transposition of an insertion element, possibly IS1558. Possible transposase Rv2177c, (MTV021.10c), len: 221 aa. Possible IS1558 transposase (see citation below), similar to several is element proteins and transposases but nearly identical to last 221 residues of MTCY428_23 (333 aa). FASTA scores: Z81451|MTCY428_23 Mycobacterium tuberculosis cosmid (333 aa) opt: 1491, E() : 0; 98.6% identity in 221 aa overlap. O53511 No insertion seqs and phages O53511 O53511 GO:0004803,GO:0006313,GO:0003677 O53511 NC_000962.3 Mycobrowser_v5 CDS 2441811 2442317 . - 0 Rv2179c Rv2179c Unknown Conserved hypothetical protein Rv2179c, (MTV021.12c), len: 168 aa. Conserved hypothetical protein, equivalent to conserved hypothetical protein from Mycobacterium leprae ML0895 conserved hypothetical protein (171 aa). FASTA scores: opt: 977, E(): 1.4e-58; 82.530% identity in 166 aa overlap (AL022602). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WJ73 No conserved hypotheticals P9WJ73 P9WJ73 NC_000962.3 Mycobrowser_v5 CDS 2443302 2444585 . + 0 Rv2181 Rv2181 Mannosyltansferase responsible for the addition of alpha(1->2) branches to the mannan core in the biosynthesis of lipomannan (LM) and lipoarabinomannan (lam) Alpha(1->2)mannosyltransferase Rv2181, (MTV021.14), len: 427 aa. Alpha(1->2)mannosyltransferase (See Kaur et al., 2006). Probable integral membrane protein, similar to others in Mycobacterium tuberculosis e.g. Rv1159 (MTCI65.26, 431 aa). Start uncertain. FASTA scores: Z95584|MTCI65_26 (431 aa) opt: 428, E(): 8e-22; 31.2% identity in 407 aa overlap. Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). P9WMZ9 No cell wall and cell processes O53515 P9WMZ9 GO:0009405,GO:0008654,GO:0005886,GO:0016757,GO:0016021 2.4.1.- P9WMZ9 NC_000962.3 Mycobrowser_v5 CDS 2445807 2446946 . - 0 Rv2184c Rv2184c Unknown Conserved hypothetical protein Rv2184c, (MTV021.17c), len: 379 aa. Conserved hypothetical protein, equivalent to hypothetical protein ML0890 (415 aa) from Mycobacterium leprae and also shows some similarity to other hypothetical proteins. FASTA scores: ML0890 opt: 1949; 79.630% identity in 378 aa overlap >emb|CAA18692.1| (AL022602) >gi|13092962|emb|CAC31271.1| (AL583920) and sptr|Q55794|Q55794 hypothetical 44.6 kDa protein. (396 aa) opt: 251, E(): 3.3e-09; 25.5% identity in 384 aa overlap. O53518 No conserved hypotheticals O53518 O53518 NC_000962.3 Mycobrowser_v5 CDS 2456901 2457512 . + 0 Rv2193 ctaE Thought to be involved in aerobic respiration. Probable cytochrome C oxidase (subunit III) CtaE Rv2193, (MTCY190.04), len: 203 aa. Probable ctaE, cytochrome c oxidase polypeptide III (cox3), with strong similarity to others e.g. COX3_SYNY3|Q06475 (29.8% identity in 225 aa overlap). P9WP67 No intermediary metabolism and respiration P63856 P9WP67 GO:0016021,GO:0006123,GO:0005886,GO:0004129 1.9.3.1 P9WP67 NC_000962.3 Mycobrowser_v5 CDS 2449993 2451150 . - 0 Rv2188c pimB Involved in lipoarabinomannan (lam) biosynthesis Mannosyltransferase PimB Rv2188c, (MTV021.21c), len: 385 aa. PimB (previously known as pimB'), mannosyltransferase. Equivalent to Mycobacterium leprae ML0886 putative glycosyl transferase (384 aa). FASTA scores: ML0886 (CAA18697.1| (AL022602) ) opt: 2113, E(): 1.8e-106; 81.462% identity in 383 aa overlap; sptr|P73369|P73369 hypothetical 46.2 kDa protein (404 aa) opt: 379, E(): 2.2e-18; 27.5% identity in 397 aa overlap. Start changed since first submission, now 14 aa shorter. P9WMZ3 No lipid metabolism O53522 P9WMZ3 GO:0043750,GO:0008654,GO:0009405 2.4.1.57 P9WMZ3 NC_000962.3 Mycobrowser_v5 CDS 2451247 2452020 . - 0 Rv2189c Rv2189c Unknown Conserved hypothetical protein Rv2189c, (MTV021.22c), len: 257 aa. Conserved hypothetical protein; some similarity to hypothetical protein SC6G10.07c (385 aa) from Streptomyces coelicolor A3(2). Smith-Waterman scores: pir||T35516 hypothetical protein SC6G10.07c - Streptomyces coelicolor >gi|4539203|emb|CAB39861.1| (AL049497) Expect = 2e-08; 30% identity in 245 aa overlap. O53523 No conserved hypotheticals O53523 O53523 NC_000962.3 Mycobrowser_v5 CDS 2452115 2453272 . - 0 Rv2190c Rv2190c Unknown Conserved hypothetical protein Rv2190c, (MTV021.23c, MTCY190.01c), len: 385 aa. Conserved hypothetical protein; similar to other hypothetical mycobacterial proteins, including Rv1477, Rv1478, Rv1566c, Rv0024, that are similar to protein p60 precursors from Listeria e.g. Q018 38|P60_LISSE protein p60 precursor (invasion-associated protein) (524 aa). FASTA scores: gp|Z80233|MTCY10H4_25 (281 aa) opt: 290, E(): 6.9e-05; 37.0% identity in 127 aa overlap and sp|Q01838|P60_LISSE protein P60 precursor (523 aa) opt: 268, E(): 0.00071; 38.5% identity in 104 aa overlap. P9WHU3 No virulence, detoxification, adaptation P67473 P9WHU3 P9WHU3 NC_000962.3 Mycobrowser_v5 CDS 2464997 2466955 . + 0 Rv2201 asnB Asparagine biosynthesis Probable asparagine synthetase AsnB Rv2201, (MTCY190.12), len: 652 aa. Probable asnB, asparagine synthetase, similar to e.g. SW:ASNH_BACSU P42113 putative asparagine synthetase (26.0% identity in 438 aa overlap) P9WN33 No intermediary metabolism and respiration P64247 P9WN33 GO:0006529,GO:0004066,GO:0006541,GO:0005524 6.3.5.4 P9WN33 NC_000962.3 Mycobrowser_v5 CDS 2455631 2456743 . - 0 Rv2192c trpD Tryptophan biosynthesis Probable anthranilate phosphoribosyltransferase TrpD Rv2192c, (MTCY190.03c), len: 370 aa. Probable trpD, anthranilate phosphoribosyltransferase (see citation below), similar to e.g. TRPD_LACCA|P17170, (43.2% identity in 308 aa overlap). Initiation codon uncertain, gtg at 4086 in MTCY190 favoured by homology but this has no clear ribosome binding site. P9WFX5 No intermediary metabolism and respiration 1ZVW,2BPQ,3QR9 P66992 P9WFX5 GO:0000162,GO:0004048 2.4.2.18 P9WFX5 NC_000962.3 Mycobrowser_v5 CDS 2457553 2458395 . + 0 Rv2194 qcrC Respiration Probable ubiquinol-cytochrome C reductase QcrC (cytochrome C subunit) Rv2194, (MTCY190.05), len: 280 aa. Probable qcrC, Ubiquinol-cytochrome C reductase cytochrome C subunit (cyoA), shows similarity to cytochrome c family; contains 2 X PS00190 Cytochrome c family heme-binding site signature. P9WP35 No intermediary metabolism and respiration P63887 P9WP35 GO:0022900,GO:0020037,GO:0016021,GO:0005886,GO:0006810,GO:0009055 P9WP35 NC_000962.3 Mycobrowser_v5 CDS 2458392 2459681 . + 0 Rv2195 qcrA Respiration Probable rieske iron-sulfur protein QcrA Rv2195, (MTCY190.06), len: 429 aa. Probable qcrA, Ubiquinol-cytochrome C reductase iron-sulfur subunit (cyoB), shows some similarity to cytochrome B6-F complex iron-sulphur subunits (Rieske iron-sulfur protein); contains PS00200 Rieske iron-sulfur protein signature 2 P9WH23 No intermediary metabolism and respiration Q10387 P9WH23 GO:0022900,GO:0016021,GO:0046872,GO:0016491,GO:0005886,GO:0006810,GO:0051537 P9WH23 NC_000962.3 Mycobrowser_v5 CDS 2462148 2463047 . - 0 Rv2198c mmpS3 Unknown Probable conserved membrane protein MmpS3 Rv2198c, (MTCY190.09c), len: 299 aa. Probable mmpS3, conserved membrane protein (see citation below), equivalent to ML0877|mmpS3 putative membrane protein from Mycobacterium leprae (293 aa), FASTA scores: opt: 1089, E(): 1.2e-43, (69.80% identity in 308 aa overlap). Also similar to other proteins e.g. Rv3209 from Mycobacterium tuberculosis. Contains PS00499 C2 domain signature, a hydrophobic region, and a repetitive proline and threonine rich region. Belongs to the MmpS family. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WJT1 No cell wall and cell processes P65378 P9WJT1 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2463233 2463652 . - 0 Rv2199c Rv2199c Unknown Possible conserved integral membrane protein Rv2199c, (MTCY190.10c), len: 139 aa. Possible conserved integral membrane protein, similar to hypothetical membrane proteins in Actinomycetes and equivalent to Mycobacterium leprae, ML0876, putative membrane protein (139 aa) FASTA scores: opt: 866, E(): 1.1e-43; 91.367% identity in 139 aa overlap CAC31257.1| (AL583920) P9WP45 No cell wall and cell processes P64947 P9WP45 GO:0016021,GO:0055114,GO:0005886,GO:0004129 1.9.3.1 P9WP45 NC_000962.3 Mycobrowser_v5 CDS 2463660 2464751 . - 0 Rv2200c ctaC Involved in aerobic respiration. Subunit I and II form the functional core of the enzyme complex. Electrons originating in cytochrome C are transferred via heme a and Cu(A) to the binuclear center formed by heme A3 and Cu(B). Probable transmembrane cytochrome C oxidase (subunit II) CtaC Rv2200c, (MTCY190.11c), len: 363 aa. Probable ctaC, transmembrane cytochrome C oxidase (subunit II), COX2, similar e.g. to JT0964 cytochrome-c oxidase chain II (23.0% identity in 317 aa overlap); etc. Contains PS00078 Cytochrome c oxidase subunit II, copper a binding region signature. Belongs to the cytochrome C oxidase subunit 2 family. P9WP69 No intermediary metabolism and respiration P63854 P9WP69 GO:0004129,GO:0009055,GO:0020037,GO:0016021,GO:0005886,GO:0070469,GO:0022904,GO:0006810,GO:0005507 1.9.3.1 P9WP69 NC_000962.3 Mycobrowser_v5 CDS 2500931 2501632 . + 0 Rv2227 Rv2227 Unknown Conserved hypothetical protein Rv2227, (MTCY427.08), len: 233 aa. Conserved hypothetical protein, similar to conserved hypothetical proteins from various bacteria e.g. gb|AAK22693.1| (AE005746) conserved hypothetical protein from Caulobacter crescentus (234 aa) Smith-Waterman score = 109 bits (429), Expect = 1e-41 Identities = 83/167 (49%) P9WLH7 No conserved hypotheticals Q10511 P9WLH7 P9WLH7 NC_000962.3 Mycobrowser_v5 CDS 2467053 2468027 . - 0 Rv2202c adoK Adenosine phosphorylation Adenosine kinase Rv2202c, (MTCY190.13c), len: 324 aa. AdoK, Adenosine kinase activity proven biochemically (See Long et al. 2003). Similar to several others but shows greater sequence homology with ribokinase and fructokinase than it does with other AKs e.g. AE000915_1 Methanobacterium thermoautotrop (309 aa) FASTA score: opt: 370, E(): 3.3e-18; 31.2% identity in 276 aa overlap. Low similarity to carbohydrate kinases, e.g. SW:RBSK_BACSU P36945 ribokinase (23.9% identity in 272 aa overlap); contains PS00583 pfkB family of carbohydrate kinases signature 1. Previously known as cbhK P9WID5 No intermediary metabolism and respiration 2PKF,2PKK,2PKM,2PKN P83734 P9WID5 GO:0004001,GO:0006166,GO:0005524 2.7.1.20 P9WID5 NC_000962.3 Mycobrowser_v5 CDS 2468931 2469287 . - 0 Rv2204c Rv2204c Unknown Conserved protein Rv2204c, (MTCY190.15c), len: 118 aa. Conserved protein. Similar to conserved hypothetical proteins in Actinomycetes and equivalent to Mycobacterium leprae ML0871|ML0871 conserved hypothetical protein (118 aa) and to sp|P45344|YADR_HAEIN hypothetical protein HI1723 (114 aa). FASTA score: ML0871 opt: 720, E(): 8.4e-45; 92.373% identity in 118 aa overlapCAC31252.1| (AL583920); and P45344 opt: 346, E(): 1.8e-18; 45.6% identity in 103 aa overlap. Contains PS01152 Hypothetical hesB/y yadR/yfhF family signature P9WMN5 No conserved hypotheticals P0A5A9 P9WMN5 GO:0051536,GO:0005198,GO:0016226 P9WMN5 NC_000962.3 Mycobrowser_v5 CDS 2469387 2470463 . - 0 Rv2205c Rv2205c Unknown Conserved hypothetical protein Rv2205c, (MTCY190.16c), len: 358 aa. Conserved hypothetical protein. Very similar to YHAD_ECOLI|P23524 hypothetical protein (YHAD (E.coli) / YXAA (S14A) (B.subtilis) family) (41.6% identity in 154 aa overlap), and to other members of the glycerate kinase family. Start changed since first submission; protein now 122 aa shorter, owing to extension of Rv2206. Nucleotide position 2470149 in the genome sequence has been corrected, T:C resulting in E105E. P9WMT7 No conserved hypotheticals P64288 P9WMT7 GO:0031388,GO:0008887 P9WMT7 NC_000962.3 Mycobrowser_v5 CDS 2473400 2474938 . + 0 Rv2209 Rv2209 Unknown Probable conserved integral membrane protein Rv2209, (MTCY190.20), len: 512 aa. Probable conserved integral membrane protein, similar to but longer than Rv0246 gp|AL021929|MTV 034_12 Mycobacterium tuberculosis (436 aa). FASTA score: opt: 712, E(): 2.8e- 32; 33.4% identity in 422 aa overlap P9WLI3 No cell wall and cell processes P9WLI3 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2474864 2475970 . - 0 Rv2210c ilvE Thought to catalyze the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine [catalytic activity: L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate]. Branched-chain amino acid transaminase IlvE Rv2210c, (MTCY190.21c), len: 368 aa. ilvE, Branched-chain-amino-acid transaminase, highly similar to many e.g. YWAA_BACSU|P39576 from Bacillus subtilis (48.4% identity in 339 aa overlap); etc. P9WQ75 No intermediary metabolism and respiration 3HT5 Q10399 P9WQ75 GO:0004084,GO:0009082 2.6.1.42 P9WQ75 NC_000962.3 Mycobrowser_v5 CDS 2476042 2477181 . - 0 Rv2211c gcvT The glycine cleavage system catalyzes the degradation of glycine [catalytic activity: (6S)-tetrahydrofolate + S-aminomethyldihydrolipoylprotein = (6R)-5,10-methylenetetrahydrofolate + NH3 + dihydrolipoylprotein]. Probable aminomethyltransferase GcvT (glycine cleavage system T protein) Rv2211c, (MTCY190.22), len: 379 aa. Probable gcvT, aminomethyltransferase, similar to many e.g. GCST_ECOLI|P27248 for Escherichia coli (38.2% identity in 364 aa overlap); etc. Belongs to the GcvT family. P9WN51 No intermediary metabolism and respiration P64220 P9WN51 GO:0005737,GO:0019464,GO:0008483,GO:0004047 2.1.2.10 P9WN51 NC_000962.3 Mycobrowser_v5 CDS 2486235 2486987 . + 0 Rv2219 Rv2219 Unknown Probable conserved transmembrane protein Rv2219, (MTCY190.30), len: 250 aa. Probable conserved transmembrane protein. Equivalent to hypothetical membrane protein ML0857 (250 aa) from Mycobacterium leprae Z98741 |MLCB22_13 Mycobacterium leprae cosmid B22; H (250 aa) opt : 1328, E(): 0; 80.8% identity in 250 aa overlap. P9WLI1 No cell wall and cell processes Q10405 P9WLI1 GO:0005886,GO:0016021 P9WLI1 NC_000962.3 Mycobrowser_v5 CDS 2510715 2511176 . - 0 Rv2238c ahpE Detoxification of organic peroxides. Probable peroxiredoxin AhpE Rv2238c, (MTCY427.19c), len: 153 aa. Probable ahpE, peroxiredoxin. Similarity to many members of AHPC/TSA family e.g. sp|Q96291|BAS1_ARATH 2-CYS peroxiredoxin BAS1 precursor (265 aa). FASTA score: opt: 275, E(): 2.7e-12; 35.0% identity in 143 aa overlap. P9WIE3 No virulence, detoxification, adaptation 1XVW,1XXU P65688 P9WIE3 GO:0055114,GO:0004601,GO:0051920,GO:0045454 1.11.1.15 P9WIE3 NC_000962.3 Mycobrowser_v5 CDS 2854683 2854907 . - 0 Rv2530A vapB39 Unknown Possible antitoxin VapB39 Rv2530A, len: 74 aa. Possible vapB39, antitoxin, part of toxin-antitoxin (TA) operon with Rv2530c, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. O53218|Rv2493 (73 aa), FASTA scores: opt: 240, E(): 5.7e-11, (56.75% identity in 74 aa overlap); and Q92WE1|RB0399|SMB20413 hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti)p lasmid pSymB (megaplasmid 2) (75 aa), FASTA scores: opt: 226, E(): 6.5e-10, (56.00% identity in 75 aa overlap). P9WJ23 No virulence, detoxification, adaptation P9WJ23 NC_000962.3 Mycobrowser_v5 CDS 2479923 2481701 . - 0 Rv2214c ephD Thought to be involved in detoxification reactions following oxidative damage to lipids. Possible short-chain dehydrogenase EphD Rv2214c, (MTCY190.25c), len: 592 aa. Possible ephD, short-chain dehydrogenase (see citation below), equivalent to Z98741|MLCB22_8 Mycobacterium leprae cosmid B22; (596 aa), FASTA score: opt: 3262, E(): 0; 80.4% identity in 596 aa overlap. C-terminus similar to short-chain alcohol dehydrogenase family, similar to SW:LIGD_PSEPA Q01198 c alpha-dehydrogenase (30.7% identity in 241 aa overlap); contains PS00061 Short-chain alcohol dehydrogenase family signature, PS00697 ATP-dependent DNA ligase AMP-binding site. N-terminus corresponds to several epoxide hydrolases of plants and Mycobacterium tuberculosis e.g. MTCY9F925 P9WGS3 No virulence, detoxification, adaptation P66777 P9WGS3 GO:0055114,GO:0016491 1.-.-.- P9WGS3 NC_000962.3 Mycobrowser_v5 CDS 2483626 2484531 . + 0 Rv2216 Rv2216 Unknown Conserved protein Rv2216, (MTCY190.27), len: 301 aa. Conserved protein, equivalent to Mycobacterium leprae ML0860 (307 aa), Z98741|MLCB22_10 Mycobacterium leprae cosmid B22; H (307 aa). FASTA score: opt: 1656, E(): 0; 84.2% identity in 297 aa overlap. Also gp|AE000319|ECAE000319_8 Escherichia coli strain K12 MG1655 (297 aa) opt: 640, E(): 0; 39.5% identity in 294 aa overlap. P9WGP7 No conserved hypotheticals P67232 P9WGP7 GO:0044237,GO:0050662,GO:0003824 P9WGP7 NC_000962.3 Mycobrowser_v5 CDS 2484584 2485276 . + 0 Rv2217 lipB Lipoate biosynthesis Probable lipoate biosynthesis protein B LipB Rv2217, (MTCY190.28), len: 230 aa. Probable lipB, similar to SW:LIPB_ECOLI P30976 liopate biosynthesis protein B (33.8% identity in 160 aa overlap). Equivalent to gp|Z98741| MLCB22_11 Mycobacterium leprae (235 aa). FASTA score: opt: 1124, E(): 0; 78.4% identity in 218 aa overlap P9WK83 No intermediary metabolism and respiration 1W66 Q10404 P9WK83 GO:0009107,GO:0033819,GO:0016415,GO:0006464,GO:0005737 2.3.1.181 P9WK83 NC_000962.3 Mycobrowser_v5 CDS 2485273 2486208 . + 0 Rv2218 lipA Lipoate biosynthesis Probable lipoate biosynthesis protein A LipA Rv2218, (MTCY190.29), len: 311 aa. Probable lipA, lipoic acid synthetase, similar to e.g. SW:LIPA_HAEIN P44463 (42.6% identity in 291 aa overlap). Equivalent to Z98741|MLCB2 2_12 Mycobacterium leprae cosmid B22; (314 aa). FASTA score : opt: 1836, E(): 0; 86.8% identity in 310 aa overlap P9WK91 No intermediary metabolism and respiration P65283 P9WK91 GO:0005737,GO:0009107,GO:0016992,GO:0046872,GO:0051539 2.8.1.8 P9WK91 NC_000962.3 Mycobrowser_v5 CDS 2492402 2493742 . - 0 Rv2222c glnA2 Involved in glutamine biosynthesis [catalytic activity: ATP + L-glutamate + NH(3) = ADP + glutamine + orthophosphate]. Probable glutamine synthetase GlnA2 (glutamine synthase) (GS-II) Rv2222c, (MTCY427.03c), len: 446 aa. Probable glnA2, glutamine synthetase class II, similar to others. Also similar to three other potential glutamine synthetases in Mycobacterium tuberculosis: Rv2220|glnA1, Rv2860c|glnA4, and Rv1878|glnA3. Belongs to the glutamine synthetase family. P9WN37 No intermediary metabolism and respiration P64245 P9WN37 GO:0005737,GO:0004356,GO:0006542,GO:0009399,GO:0005524 6.3.1.2 P9WN37 NC_000962.3 Mycobrowser_v5 CDS 2493837 2495399 . - 0 Rv2223c Rv2223c Function unknown; thought to hydrolyze peptides and/or proteins. Probable exported protease Rv2223c, (MTCY427.04c), len: 520 aa. Probable exported protease ; has signal sequence. Very similar to three proteases/peptidases from Streptomyces spp.: L42758, L42759, L27466. FASTA score: L42758|STMSLPD STMSLPD NID: g940302 - Streptomyces (539 aa) opt: 1032 E(): 0, (37.5% identity in 533 aa overlap). Also similar to hypothetical proteins YZZE _ECOLI|P34211 from Escherichia coli (25.4% identity in 406 aa overlap) and PIR:B36944 in ompP 3' region (27.5% identity in 218 aa overlap). Highly similar to Rv2224c and Rv2672 (49.3% identity in 507 aa overlap); contains PS00120 Lipases, serine active site. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). Predicted to be an outer membrane protein (See Song et al., 2008). P9WHR5 No cell wall and cell processes P65821 P9WHR5 GO:0005886,GO:0016021 P9WHR5 NC_000962.3 Mycobrowser_v5 misc_RNA 2500445 2500751 . - 0 MTB000026 rnpB RNA component of RNase P: RNase P catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'terminus. This protein plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme. Ribonuclease P RNA rnpB, rna component of RNase P. No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 2498832 2500373 . + 0 Rv2226 Rv2226 Unknown Conserved protein Rv2226, (MTCY427.07), len: 513 aa. Conserved protein, similar to hypothetical secreted protein (510 aa) from Streptomyces coelicolor A3(2) emb|CAB59601.1| (AL132662) hypothetical secreted protein [Streptomyces coelicolor. Smith-Waterman scores Expect = 5e-44 Identities = 166/506 (32%) P9WLH9 No conserved hypotheticals Q10510 P9WLH9 GO:0006171,GO:0004016 P9WLH9 NC_000962.3 Mycobrowser_v5 CDS 2502735 2503472 . - 0 Rv2229c Rv2229c Unknown Conserved protein Rv2229c, (MTCY427.10c), len: 245 aa. Conserved protein; probable coiled-coil protein similar to conserved hypothetical proteins in Actinomycetes. Equivalent to Mycobacterium leprae ML1638 (232 aa), FASTA scores: opt: 868 E(): 4.4e-43; 60.870% identity in 230 aa overlap emb|CAC30589.1| (AL583922). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WLH3 No conserved hypotheticals Q10513 P9WLH3 P9WLH3 NC_000962.3 Mycobrowser_v5 CDS 2503469 2504608 . - 0 Rv2230c Rv2230c Unknown Conserved protein Rv2230c, (MTCY427.11c), len: 379 aa. Conserved protein. Equivalent to Mycobacterium leprae, ML1639, conserved hypothetical protein (385 aa). Similar to hypothetical proteins from B. subtilis, P54472, and L. monocytogenes, P53434. FASTA score: ML1639 (MLCB1243.36) opt: 2088, E(): 4e-107; 79.481% identity in 385 aa overlap same as >pir||T44719 hypothetical protein MLCB1243.36 [imported] - Mycobacterium leprae >gi|3150237|emb|CAA19217.1| (AL023635); P54472|YQFO_BACSU hypothetical 30. 7 kDa protein in (279 aa) opt: 604; E(): 2.2e-30; 38.8% identity in 258 aa overlap. P53434|YRP2_LISMO hypothetical 41.4 kDa protein (373 aa) opt: 595, E(): 1e-29; 30.7% identity in 326 aa overlap P9WFM1 No conserved hypotheticals P0A656 P9WFM1 P9WFM1 NC_000962.3 Mycobrowser_v5 CDS 2511690 2512280 . - 0 Rv2240c Rv2240c Unknown Unknown protein Rv2240c, (MTCY427.21c), len: 196 aa. Unknown protein. Start changed since first submission (-69 aa). P9WLG7 No conserved hypotheticals P9WLG7 GO:0016021 P9WLG7 NC_000962.3 Mycobrowser_v5 CDS 2541644 2542810 . - 0 Rv2267c Rv2267c Unknown Conserved hypothetical protein Rv2267c, (MTCY339.43), len: 388 aa. Conserved hypothetical protein; some similarity to Mycobacterium tuberculosis Rv3529c; gp|Z82098|MTCY3C7_27 (384 aa) FASTA score: opt: 261, E(): 3.6e-10; 27.3% identity in 253 aa overlap. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLG1 No conserved hypotheticals P64963 P9WLG1 GO:0008146 P9WLG1 NC_000962.3 Mycobrowser_v5 CDS 2564029 2564253 . - 0 Rv2292c Rv2292c Unknown Hypothetical protein Rv2292c, (MTCY339.18), len: 74 aa. Unknown hypothetical protein P9WLE3 No unknown P64977 P9WLE3 GO:0009116,GO:0003824 NC_000962.3 Mycobrowser_v5 CDS 2505736 2506161 . - 0 Rv2231A vapC16 Unknown Possible toxin VapC16 Rv2231A, len: 141 aa. Possible vapC16, toxin, part of toxin-antitoxin (TA) operon with Rv2231B (See Pandey and Gerdes, 2005). Nucleotide position 2505919 in the genome sequence has been corrected, A:G resulting in A81A. P0CV93 No virulence, detoxification, adaptation P0CV93 NC_000962.3 Mycobrowser_v5 CDS 2507637 2508452 . + 0 Rv2235 Rv2235 Function unknown: may be involved in the ability to survive in macrophages. Probable conserved transmembrane protein Rv2235, (MTCY427.16), len: 271 aa. Probable conserved transmembrane protein (see Miller & Shinnick 2001); hydrophobic regions near N- and C-terminus. Similar to conserved membrane proteins in other Actinomycetes. Equivalent to Mycobacterium leprae. ML1644 (270 aa). FASTA scores: opt: 1357, E(): 1.2e-72; 74.170% identity in 271 aa overlap T44717|3150235|CAA19213.1|AL023635 13093419|CAC30595.1|AL583922. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WGA7 No cell wall and cell processes P66883 P9WGA7 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2508434 2509375 . - 0 Rv2236c cobD Involved in cobalamin biosynthesis, in the conversion of cobyric acid to cobinamide. Probable cobalamin biosynthesis transmembrane protein CobD Rv2236c, (MTCY427.17c), len: 313 aa. Probable cobD, cobalamin biosynthesis transmembrane protein, similar to S52223 Rhodobacter capsulatus 945 protein BluD (39.0% identity in 287 aa overlap) involved in cobinamide synthesis, and to COBD_PSEDE Pseudomonas dentrificans cobD protein (37.5% identity in 269 aa overlap), also CBIB_SALTY Salmonella typhimurum cbiB protein (35.5% identity in 304 aa overlap). P9WP93 No intermediary metabolism and respiration Q10518 P9WP93 GO:0015420,GO:0016021,GO:0005886,GO:0009236 NC_000962.3 Mycobrowser_v5 CDS 2509489 2510256 . + 0 Rv2237 Rv2237 Unknown Conserved protein Rv2237, (MTCY427.18), len: 255 aa. Conserved protein. Similar to Mycobacterium tuberculosis hypothetical proteins Rv0276, Rv0826, Rv1645c. FASTA score: Rv0276 gp|AL021930|MTV035_4 (306 aa) opt: 874, E(): 0; 49.6% identity in 282 aa overlap P9WLH1 No conserved hypotheticals P64957 P9WLH1 GO:0005886,GO:0016021 P9WLH1 NC_000962.3 Mycobrowser_v5 CDS 2511176 2511652 . - 0 Rv2239c Rv2239c Unknown Conserved hypothetical protein Rv2239c, (MTCY427.20c), len: 158 aa. Conserved hypothetical protein, similar to conserved hypothetical proteins from Mycobacterium leprae (ML1649, 140 aa) and Streptomyces coelicolor A3(2) (SCC8A.28c, 159 aa). Equivalent to ML1649 conserved hypothetical protein (140 aa). FASTA scores: ML1649 conserved hypothetical protein (140 aa) opt: 846, E(): 6.5e-45; 86.429% identity in 140 aa overlap (tr|O69479|O69479 hypothetical 15.2 KDA protein (140 aa); and opt: 447, E(): 1.2e-21; 50.355% identity (51.825% ungapped) in 141 aa overlap. Similarity with ML1649 suggests alternative start at 251198. P9WLG9 No conserved hypotheticals P64959 P9WLG9 P9WLG9 NC_000962.3 Mycobrowser_v5 CDS 2515304 2516548 . + 0 Rv2242 Rv2242 Unknown Conserved hypothetical protein Rv2242, (MTCY427.23), len: 414 aa. Conserved hypothetical protein. Equivalent to ML1652 conserved hypothetical protein from Mycobacterium leprae (414 aa), and orthologue in Streptomyces coelicolor A3(2). FASTA scores: ML1652 opt: 2369, E(): 4.2e-128; 88.406% identity in 414 aa overlap (AL023635)(AL583922). Some similarity at 3' end with S25203 srmR protein - Streptomyces ambofaciens (604 aa) opt: 188 E(): 9e-05; (26.4% identity in 277 aa overlap) and with SW:YAEG_HAEIN P44509 hypothetical protein HI0093 (42.3% identity in 52 aa overlap). Contains possible helix-turn-helix motif at aa 360-381 (+3.52 SD) P9WPH5 No conserved hypotheticals P9WPH5 INH P9WPH5 NC_000962.3 Mycobrowser_v5 CDS 2517771 2518118 . + 0 Rv2244 acpM Involved in fatty acid biosynthesis (mycolic acids synthesis); involved in meromycolate extension. Meromycolate extension acyl carrier protein AcpM Rv2244, (MT2304, MTCY427.25), len: 115 aa. AcpM, acyl carrier protein, meromycolate precursor transport, involved in meromycolate extension (see citations below). Highly similar to others e.g. L43074|STMFABD2|STMFABD|g870805 acyl carrier protein from Streptomyces glaucescens (82 aa), FASTA scores: opt: 298, E(): 8.4e-13, (56.6% identity in 76 aa overlap); and ACP_ECOLI|P02901 acyl carrier protein from Escherichia coli, FASTA score: (37.3% identity in 67 aa overlap); etc. P9WQF3 No lipid metabolism 1KLP P0A4W6 P9WQF3 GO:0048037,GO:0005737,GO:0006633,GO:0000036 P9WQF3 NC_000962.3 Mycobrowser_v5 CDS 2518115 2519365 . + 0 Rv2245 kasA Involved in fatty acid biosynthesis (mycolic acids synthesis); involved in meromycolate extension. Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP [catalytic activity: acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = 3-oxoacyl-[acyl-carrier protein] + [acyl-carrier protein] + CO(2)]. 3-oxoacyl-[acyl-carrier protein] synthase 1 KasA (beta-ketoacyl-ACP synthase) (KAS I) Rv2245, (MTCY427.26), len: 416 aa. KasA, beta-ketoacyl-ACP synthase, involved in meromycolate extension (see citations below): belongs to the fas-II system, which utilizes primarily palmitoyl-ACP rather than short-chain acyl-ACP primers. Highly similar to others e.g. L43074|STMFABD3|g870805 beta-ketoacyl-ACP synthase from Streptomyces glaucescens (423 aa), FASTA scores: opt: 1105, E(): 0, (44.6% identity in 417 aa overlap); FABF_ECOLI|P39435 3-oxoacyl-[acyl-carrier-protein] synthase II from Escherichia coli, FASTA score: (39.4% identity in 254 aa overlap); FABB_HORVU|P23902 3-oxoacyl-[acyl-carrier-protein] synthase I, FASTA score: (33.4% identity in 413 aa overlap); etc. Strongest similarity to downstream ORF kasB|Rv2246|MTCY427.27 3-oxoacyl-[acyl-carrier-protein] synthase 2 from Mycobacterium tuberculosis (438 aa), FASTA score: (66.3% identity in 409 aa overlap). Belongs to the beta-ketoacyl-ACP synthases family. P9WQD9 No lipid metabolism 2WGD,2WGE,2WGF,2WGG P63454 P9WQD9 GO:0005737,GO:0006633,GO:0004315 2.3.1.41 INH P9WQD9 NC_000962.3 Mycobrowser_v5 CDS 2520743 2522164 . + 0 Rv2247 accD6 Involved in fatty acid biosynthesis (mycolic acids synthesis) [catalytic activity: ATP + propionyl-CoA + CO(2) + H(2)O = ADP + orthophosphate + methylmalonyl-CoA]. Acetyl/propionyl-CoA carboxylase (beta subunit) AccD6 Rv2247, (MTCY427.28), len: 473 aa. AccD6, Acetyl/Propionyl CoA Carboxylase, beta subunit (see citations below), highly similar to e.g. PCCB_RHOSO|Q06101 propionyl-CoA carboxylase beta chain, FASTA score: (75.1% identity in 437 aa overlap). Similar to many other Acetyl/Propionyl CoA Carboxylases from Mycobacterium tuberculosis. Belongs to the AccD / PccB family. P9WQH5 No lipid metabolism P63407 P9WQH5 GO:0004658,GO:0005524 6.4.1.3 INH P9WQH5 NC_000962.3 Mycobrowser_v5 CDS 2522360 2523175 . + 0 Rv2248 Rv2248 Unknown Conserved hypothetical protein Rv2248, (MTCY427.29), len: 271 aa. Conserved hypothetical protein. Very similar to hypothetical M. tuberculosis proteins Rv3517, Rv1482c, Rv3555c, Rv3714c, Rv1073. FASTA score: MTCY06G11.02c MTCY6G11 NID: g1877284 - (289 aa) opt: 366 E(): 5.3e-18; (32.1% identity in 249 aa overlap). Some similarity to Mycobacterium avium protein AF002133|AF0021 339 AF002133 NID: g2183254 (346 aa) opt: 308 E(): 5.2e-14; (28.3% identity in 254 aa overlap) P9WLG5 No conserved hypotheticals P9WLG5 GO:0003677 P9WLG5 NC_000962.3 Mycobrowser_v5 CDS 2523241 2524791 . - 0 Rv2249c glpD1 Involved in aerobic respiration and oxidation of glycerol. Reduces an acceptor and generates glycerone phosphate from Sn-glycerol 3-phosphate. Possibly play a role in metabolism of riboflavin, FAD,FMN [catalytic activity: SN-glycerol 3-phosphate + acceptor = glycerone phosphate + reduced acceptor]. Probable glycerol-3-phosphate dehydrogenase GlpD1 Rv2249c, (MTCY427.31c), len: 516 aa. Probable glpD1, glycerol-3-phosphate dehydrogenase, similar to SW:GLPD_ECOLI P13035 aerobic glycerol-3-phosphate dehydrogenase (30.0% identity in 486 aa overlap) and SW:GLPA_ECOLI P13032 anaerobic glycerol-3-phosphate dehydrogenase (28.2% identity in 504 aa overlap). Also similar to Rv3302c|glpD2 glycerol-3-phosphate dehydrogenase. Cofactor: FAD (by similarity). Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family. P9WN81 No intermediary metabolism and respiration P64182 P9WN81 GO:0009331,GO:0006072,GO:0055114,GO:0004368 1.1.5.3 P9WN81 NC_000962.3 Mycobrowser_v5 CDS 2524785 2525354 . - 0 Rv2250c Rv2250c Possibly involved in transcriptional regulation Possible transcriptional regulatory protein Rv2250c, (MTCY427.32c), len: 189 aa. Possible transcriptional regulatory protein, TetR family. Start unclear; ORF has been shortened since first submission to avoid overlap with Rv2251 (-30 aa). Contains probable helix-turn-helix motif (Score 2243, +6.70 SD) P9WMC5 No regulatory proteins Q10528 P9WMC5 GO:0006350,GO:0003700,GO:0006355 P9WMC5 NC_000962.3 Mycobrowser_v5 CDS 2525402 2525821 . + 0 Rv2250A Rv2250A Electron acceptor Possible flavoprotein Rv2250A, len: 139 aa. Conserved hypothetical protein, possibly flavoprotein. Similar to N-terminus of SCF91.28c|AL132973_28 possible flavoprotein from Streptomyces coelicolor (530 aa), FASTA scores: opt: 240, E(): 1.1e-07, (39.25% identity in 107 aa overlap). Possible frameshift between nt 2525723 to 2525727. The sequences of CDC 1551 and Mycobacterium bovis are missing a single G base. L0TBY6 No intermediary metabolism and respiration L0TBY6 L0TBY6 NC_000962.3 Mycobrowser_v5 CDS 2564292 2565032 . - 0 Rv2293c Rv2293c Unknown Conserved hypothetical protein Rv2293c, (MTCY339.17), len: 246 aa. Conserved hypothetical protein; some similarity to hypothetical protein (299 aa) AAK24237.1| (AE005897) belonging to phosphorylase family [Caulobacter crescentus] (33% identity in 131 aa overlap). Possible lipoprotein: signal peptide at N-terminus P9WLE1 No conserved hypotheticals Q50673 P9WLE1 GO:0016021,GO:0009116,GO:0005886,GO:0003824 P9WLE1 NC_000962.3 Mycobrowser_v5 CDS 2526989 2527918 . + 0 Rv2252 Rv2252 Unknown. Involved in synthesis of phosphatidylinositol mannosides (PIMS). Diacylglycerol kinase Rv2252, (MTV022.02), len: 309 aa. Diacylglycerol kinase (See Owens et al., 2006), similar to hypothetical proteins from Bacillus subtilis (e.g. BSUB0004_120), Streptomyces coelicolor A3(2) >emb|CAB61184.1| (AL132973) hypothetical protein SCF91.27c (293 aa) and P39074. FASTA scores: Z99107|BSUB0004_120 Bacillus subtilis complete genome (303 aa) opt: 397, E(): 1.7e-19; (26.4% identity in 299 aa overlap) and P390 74|BMRU_BACSU BMRU protein (297 aa) opt: 309, E(): 1.3e-13; (25.0% identity in 284 aa overlap). P9WP29 No lipid metabolism O53526 P9WP29 GO:0007205,GO:0005618,GO:0004143,GO:0005576,GO:0046872,GO:0008654,GO:0005524 2.7.1.107 P9WP29 NC_000962.3 Mycobrowser_v5 CDS 2527984 2528487 . + 0 Rv2253 Rv2253 Unknown Possible secreted unknown protein Rv2253, (MTV022.03), len: 167 aa. Possible secreted protein; has potential N-terminal signal peptide. Predicted to be an outer membrane protein (See Song et al., 2008). O53527 No cell wall and cell processes O53527 NC_000962.3 Mycobrowser_v5 CDS 2528520 2528975 . - 0 Rv2254c Rv2254c Unknown Probable integral membrane protein Rv2254c, (MTV022.04c), len: 151 aa. Probable integral membrane protein. O53528 No cell wall and cell processes O53528 O53528 GO:0016021,GO:0005886,GO:0006865 NC_000962.3 Mycobrowser_v5 CDS 2529341 2529874 . - 0 Rv2256c Rv2256c Unknown Conserved hypothetical protein Rv2256c, (MTV022.06c), len: 177 aa. Conserved hypothetical protein, similar to Streptomyces glaucescens ORF5 (164 aa) and Streptomyces coelicolor hypothetical protein SC4A7.19c (164 aa; emb|CAB62723.1|AL133423). FASTA scores: sptr|Q54209|Q54209 FABD, FABH, FABC, FABB, and ORF5 (164 aa) opt: 504, E(): 3.9e-27; (44.4% identity in 162 aa overlap). O53530 No conserved hypotheticals O53530 O53530 NC_000962.3 Mycobrowser_v5 CDS 2530004 2530822 . - 0 Rv2257c Rv2257c Unknown Conserved protein Rv2257c, (MTV022.07c), len: 272 aa. Conserved protein, similar to hypothetical protein SC4A7.08 from Streptomyces coelicolor (273 aa; 58% identity in 243 aa overlap). Also similar to several putative esterases and penicillin-binding proteins in M. tuberculosis e.g. Rv1923, Rv1497, Rv2463, Rv3775, Rv1922, Rv1730c. O53531 No conserved hypotheticals O53531 O53531 O53531 NC_000962.3 Mycobrowser_v5 CDS 2530836 2531897 . - 0 Rv2258c Rv2258c Possibly involved in transcriptional regulation Possible transcriptional regulatory protein Rv2258c, (MTV022.08c), len: 353 aa. Possible transcriptional regulatory protein, similar to several hypothetical proteins from C. elegans. FASTA scores: sptr|O01593|O01593 coded for by C. elegans CDNA YK102 F (365 aa) opt: 577, E(): 6.4e-31; (30.5% identity in 341 aa overlap). Contains possible helix-turn helix motif at aa 47-68 (+3.65 SD) O53532 No regulatory proteins O53532 O53532 GO:0008168,GO:0008152 O53532 NC_000962.3 Mycobrowser_v5 CDS 2534042 2534464 . - 0 Rv2261c Rv2261c Function unknown; thought to be involved in lipid metabolism. Conserved hypothetical protein Rv2261c, (MTV022.11c), len: 140 aa. Conserved hypothetical protein, with function unknown but some similarity to C-terminal end of PCC6803 apolipoprotein N-acyltransferase from Synechocystis sp. Note that next ORF shows similarity to N-terminal part of P74055 apolipoprotein N-acyltransferase from Escherichia coli (519 aa), FASTA scores: opt: 142, E(): 0.007, (29.9% identity in 117 aa overlap), suggesting possible frameshift. Sequence of clones from two sources has been checked but no error found. I6XDW5 No lipid metabolism I6XDW5 I6XDW5 NC_000962.3 Mycobrowser_v5 CDS 2892446 2893471 . - 0 Rv2568c Rv2568c Function unknown Conserved hypothetical protein Rv2568c, (MTCY227.33), len: 341 aa. Conserved hypothetical protein, highly similar (but longer 60 aa) to Q98E75|MLR4376 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (308 aa), FASTA scores: opt: 566, E(): 4.1e-29, (40.2% identity in 291 aa overlap). P9WL95 No conserved hypotheticals P65009 P9WL95 NC_000962.3 Mycobrowser_v5 CDS 2535641 2536594 . + 0 Rv2263 Rv2263 Oxidoreduction Possible oxidoreductase Rv2263, (MTV022.13), len: 317 aa. Possible oxidoreductase, similar to several oxidoreductases. Similarity suggests alternative GTG start at 10154 but then no rbs. FASTA scores: sptr|Q544 05|Q54405 probably an NADP-dependent oxidoreductase (297 aa) opt: 487, E(): 1.1e-23; (36.1% identity in 299 aa overlap). Also similar to Mycobacterium tuberculosis Rv0068, and Rv0439c. O53537 No intermediary metabolism and respiration O53537 O53537 GO:0055114,GO:0016491 1.-.-.- O53537 NC_000962.3 Mycobrowser_v5 CDS 2536572 2538350 . - 0 Rv2264c Rv2264c Unknown Conserved hypothetical proline rich protein Rv2264c, (MTV022.14c), len: 592 aa. Conserved hypothetical Pro-rich protein, similar to hypothetical proteins Rv0312 (MTCY63.17, 620 aa and Rv0350) that has highly Pro-, Thr-rich C-terminus. Contains PS00343 Gram-positive cocci surface proteins 'anchoring' hexapeptide. FASTA scores: Z96800|MTCY63_17 Mycobacterium tuberculosis cosmid (620 aa) opt: 1075, E(): 8.8e-24; (38.9% identity in 627 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). O53538 No conserved hypotheticals O53538 O53538 GO:0005524 O53538 NC_000962.3 Mycobrowser_v5 CDS 2538700 2539929 . + 0 Rv2265 Rv2265 Unknown Possible conserved integral membrane protein Rv2265, (MTCY339.45c), len: 409 aa. Possible conserved integral membrane protein, with some similarity to others e.g. M. thermoauto. sp|O26855|O26855 conserved protein (383 aa), FASTA score: opt: 898 z-score: 1023.5 E(): 0; 38.0% identity in 384 aa overlap; Q58713 hypothetical 44.1 kDa protein 1 317 (398 aa), FASTA scores, opt: 305 E(): 1.2e-11; 22.8% identity in 382 aa overlap; also KGTP_ECOLI P17448 alpha-ketoglutarate permease (432 aa), FASTA scores, opt: 156, E(): 0.006, (24.8% identity in 416 aa overlap) P9WLG3 No cell wall and cell processes P64961 P9WLG3 GO:0005886,GO:0055085,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2550065 2550391 . + 0 Rv2278 Rv2278 Required for the transposition of the insertion element IS6110. Putative transposase for insertion sequence element IS6110 (fragment) Rv2278, (MTCY339.32c), len: 108 aa. Putative Transposase for IS6110 (fragment). Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2278 and Rv2279, the sequence UUUUAAAG (directly upstream of Rv2279) maybe responsible for such a frameshifting event (see McAdam et al., 1990). P9WKH5 No insertion seqs and phages P0C5G9 P9WKH5 GO:0004803,GO:0006313,GO:0003677 P9WKH5 NC_000962.3 Mycobrowser_v5 CDS 2635628 2635954 . + 0 Rv2354 Rv2354 Required for the transposition of the insertion sequence IS6110. Probable transposase for insertion sequence element IS6110 (fragment) Rv2354, (MTCY98.23), len: 108 aa. Putative Transposase for IS6110 (fragment). Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2354 and Rv2355, the sequence UUUUAAAG (directly upstream of Rv2355) maybe responsible for such a frameshifting event (see McAdam et al., 1990). P9WKH5 No insertion seqs and phages P0C5G9 P9WKH5 GO:0004803,GO:0006313,GO:0003677 P9WKH5 NC_000962.3 Mycobrowser_v5 CDS 2776316 2776969 . - 0 Rv2474c Rv2474c Function unknown Conserved hypothetical protein Rv2474c, (MTV008.30c), len: 217 aa. Hypothetical protein. Shows weak similarity with Q9L246|SC6D10.18c hypothetical 24.9 KDA protein from Streptomyces coelicolor (238 aa), FASTA scores: opt: 111, E(): 5.6, (30% identity in 233 aa overlap), blastp scores: Score= 135, E= 3.5e-07, P= 3.5e-07, Identities= 55/182 (30%). I6X4D6 No conserved hypotheticals I6X4D6 I6X4D6 NC_000962.3 Mycobrowser_v5 CDS 2542807 2544276 . - 0 Rv2268c cyp128 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Probable cytochrome P450 128 Cyp128 Rv2268c, (MT2330, MTCY339.42), len: 489 aa. Probable cyp128, cytochrome P450, similar to (but longer than) cytochrome p-450 e.g. CPXK_SACER P3 3271 cytochrome p-450 107b1 (405 aa), FASTA scores, opt: 620, E(): 8.3e-33, (31.8% identity in 406 aa overlap); contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature, similar to MTCY50.26, 32.7% identity in 382 aa overlap. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WPN7 No intermediary metabolism and respiration P63713 P9WPN7 GO:0020037,GO:0016021,GO:0004497,GO:0055114,GO:0005886,GO:0009055 1.14.-.- P9WPN7 NC_000962.3 Mycobrowser_v5 CDS 2547749 2548939 . + 0 Rv2276 cyp121 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. It has been shown to bind tighly to a range of azole-based antifungal drugs (e.g. miconazole, clotrimazole). Cytochrome P450 121 Cyp121 Rv2276, (MT2336, MTCY339.34c), len: 396 aa. Cyp121, cytochrome P450 (see citation below), similar to e.g. G303644 (397 aa) opt: 675, z-score: 776.4, E(): 2.7e-36, (33.7% identity in 407 aa overlap); contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature, similar to MTCY339.42, 29.2% identity in 298 aa overlap. P9WPP7 No intermediary metabolism and respiration 1N40,1N4G,2IJ5,2IJ7,3CXV,3CXX,3CXY,3CXZ,3CY0,3CY1,3G5F,3G5H P0A514 P9WPP7 GO:0009055,GO:0020037,GO:0004497,GO:0055114,GO:0005737 1.14.-.- P9WPP7 NC_000962.3 Mycobrowser_v5 CDS 2549124 2550029 . - 0 Rv2277c Rv2277c Unknown Possible glycerolphosphodiesterase Rv2277c, (MTCY339.33), len: 301 aa. Possible glycerolphosphodiesterase, similar to e.g. UGPQ_ECOLI P10908 glycerophosphoryldiester phosphodiesterase (cytosolic) (247 aa), FASTA scores, opt: 149, E(): 0.0061, (27.2% identity in 195 aa overlap). Start of protein uncertain, encoded by neighbouring IS6110 as given, is intact in Mycobacterium tuberculosis CDC1551 P9WLF1 No intermediary metabolism and respiration Q50687 P9WLF1 GO:0008889,GO:0006629,GO:0006071 P9WLF1 NC_000962.3 Mycobrowser_v5 CDS 2550340 2551326 . + 0 Rv2279 Rv2279 Required for the transposition of the insertion element IS6110. Probable transposase Rv2279, (MTCY339.31c), len: 328 aa. Probable IS6110 transposase. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2278 and Rv2279, the sequence UUUUAAAG (directly upstream of Rv2279) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 16 aa). P9WKH9 No insertion seqs and phages P0C5G8 P9WKH9 GO:0015074,GO:0006310,GO:0032196,GO:0003677 P9WKH9 NC_000962.3 Mycobrowser_v5 CDS 2551560 2552939 . + 0 Rv2280 Rv2280 Oxidoreduction Probable dehydrogenase Rv2280, (MTCY339.30c), len: 459 aa. Probable dehydrogenase. Similar to D-lactate dehydrogenase (cytochrome) precursor e.g. G1061264 (587 aa), FASTA scores, opt: 645,E(): 1.3e-31, (28.0% identity in 478 aa overlap), similar to MTCY50.25, 36.5% identity in 447 aa overlap P9WIT1 No intermediary metabolism and respiration Q50685 P9WIT1 GO:0055114,GO:0016491,GO:0050660 1.-.-.- P9WIT1 NC_000962.3 Mycobrowser_v5 CDS 2585052 2585906 . - 0 Rv2313c Rv2313c Unknown Hypothetical protein Rv2313c, (MTCY3G12.21), len: 284 aa. Hypothetical unknown protein. P9WLB7 No conserved hypotheticals P9WLB7 P9WLB7 NC_000962.3 Mycobrowser_v5 CDS 2625888 2626172 . - 0 Rv2346c esxO Function unknown Putative ESAT-6 like protein EsxO (ESAT-6 like protein 6) Rv2346c, (MT2411, MTCY98.15c), len: 94 aa. EsxO, ESAT-6 like protein (see citation below), member of Mycobacterium tuberculosis protein family with O53942|Rv1793|MTV049.15, O05300|Rv1198|MTCI364.10, MTCY15C10.33, P96364|MTCY07H7B.03|Rv1037c|MTCY10G2.12, MTCI364.10, etc. Belongs to the ESAT6 family. P9WNI7 No cell wall and cell processes 3OGI P9WNI7 P9WNI7 NC_000962.3 Mycobrowser_v5 CDS 2554938 2555876 . - 0 Rv2282c Rv2282c Unknown Probable transcription regulator (LysR family) Rv2282c, (MTCY339.28), len: 312 aa. Probable transcriptional regulator, lysR family, similar to others e.g. YC30_CYAPA|P48271 hypothetical transcriptional regulator YCF30 (324 aa), FASTA scores: opt: 292, E(): 4e-12, (27.6% identity in 286 aa overlap); etc. Also similar to Rv0377|MTCY39.34 from Mycobacterium tuberculosis, FASTA score: (25.4% identity in 268 aa overlap). Contains PS00044 Bacterial regulatory proteins, lysR family signature, and contains helix-turn-helix motif at aa 24 -45 (+4.93 SD). P9WMF3 No regulatory proteins P67667 P9WMF3 GO:0006350,GO:0003700,GO:0006355 P9WMF3 NC_000962.3 Mycobrowser_v5 CDS 2556145 2557440 . + 0 Rv2284 lipM Hydrolysis of lipids (bound ester). Probable esterase LipM Rv2284, (MTCY339.26c), len: 431 aa. Probable lipM, esterase, similar to others e.g. gp|Z95844|MTCY493_28 from Mycobacterium tuberculosis cosmid (420 aa), FASTA scores: opt: 1266, E(): 0, (50.1% identity in 411 aa overlap). Some similarity to G537514 arylacetamide deacetylase (399 aa), FASTA scores: opt: 190, E(): 5.9e-05, (30.4% identity in 138 aa overlap). Q50681 No intermediary metabolism and respiration Q50681 Q50681 GO:0016787 3.1.-.- Q50681 NC_000962.3 Mycobrowser_v5 CDS 2557473 2558810 . + 0 Rv2285 Rv2285 May be involved in synthesis of triacylglycerol Possible triacylglycerol synthase (diacylglycerol acyltransferase) Rv2285, (MTCY339.25c), len: 445 aa. Possible triacylglycerol synthase (See Daniel et al., 2004), member of Mycobacterium tuberculosis 15-membered protein family including Rv3740c, Rv3734c, Rv1425, Rv1760, Rv0895, Rv3480c. FASTA scores: gp|Z95844|MTCY493_29 Mycobacterium tuberculosis cosmid (459 aa) opt: 640, E(): 0; 33.4% identity in 470 aa overlap. P9WKB5 No lipid metabolism P67206 P9WKB5 GO:0006071,GO:0008610,GO:0004144 2.3.1.20 NC_000962.3 Mycobrowser_v5 CDS 2558877 2559569 . - 0 Rv2286c Rv2286c Unknown Conserved hypothetical protein Rv2286c, (MTCY339.24), len: 230 aa. Conserved hypothetical protein. Similar to Mycobacterium tuberculosis hypothetical protein, Rv2466c, AL021246|MTV008_22 (207 aa). FASTA score: opt: 324, E(): 8.9e-15; 30.4% identity in 194 aa overlap P9WLE7 No conserved hypotheticals Q50679 P9WLE7 GO:0015035,GO:0030288 P9WLE7 NC_000962.3 Mycobrowser_v5 CDS 2559703 2561331 . + 0 Rv2287 yjcE Possibly involved in transport of NA+/H+ across the membrane. Probable conserved integral membrane transport protein YjcE Rv2287, (MTCY339.23c), len: 542 aa. Probable yjcE, conserved integral membrane transport protein, similar to eukaryote NA+/H+ exchangers e.g. YJCE_ECOLI|P32703|B4065 Putative Na(+)/H(+) exchanger from Escherichia coli (549 aa), FASTA scores: opt: 436, E(): 5.6e-21, (29.4% identity in 555 aa overlap); etc. Seems to belong to CPA1 family (NA(+)/H(+) exchanger family). P9WJI3 No cell wall and cell processes P65526 P9WJI3 GO:0005886,GO:0006814,GO:0015299,GO:0055085,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2561328 2561705 . + 0 Rv2288 Rv2288 Unknown Hypothetical protein Rv2288, (MTCY339.22c), len: 125 aa. Unknown hypothetical protein P9WLE5 No conserved hypotheticals P9WLE5 NC_000962.3 Mycobrowser_v5 CDS 2877831 2878541 . + 0 Rv2558 Rv2558 Function unknown; thought to be involved in the persistence in the host. Conserved protein Rv2558, (MTCY9C4.10c), len: 236 aa. Conserved protein, highly similar to downstream ORF Q50741|MTCY9C4.11c|Rv2557|MT2645 conserved hypothetical protein from Mycobacterium tuberculosis (224 aa), FASTA scores: opt: 1007, E(): 4.7e-59, (69.2% identity in 224 aa overlap); and Mb2588 in Mycobacterium bovis (236 aa). P9WLA3 No conserved hypotheticals P9WLA3 P9WLA3 NC_000962.3 Mycobrowser_v5 CDS 4149091 4149480 . - 0 Rv3705A Rv3705A Function unknown Conserved hypothetical proline rich protein Rv3705A, len: 129 aa. Conserved hypothetical protein, similar to downstream ORF O69674|Rv3706c|MTV025.054c conserved hypothetical proline rich protein from Mycobacterium tuberculosis (106 aa), FASTA scores: opt: 245, E(): 0.00013, (40.7% identity in 113 aa overlap). I6YGY9 No conserved hypotheticals I6YGY9 I6YGY9 NC_000962.3 Mycobrowser_v5 CDS 2562599 2563114 . + 0 Rv2290 lppO Unknown Probable conserved lipoprotein LppO Rv2290, (MTCY339.20c), len: 171 aa. Probable lppO, conserved lipoprotein, similar to Rv3763, 19KD_MYCTU P11572 19 kDa lipoprotein antigen precursor (159 aa) FASTA scores, opt: 119, E (): 1.3, (25.6% identity in 164 aa overlap). Contains appropriately positioned PS00013 lipoprotein motif (with one mismatch). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WK71 No cell wall and cell processes P9WK71 GO:0005886 P9WK71 NC_000962.3 Mycobrowser_v5 CDS 2563174 2564028 . + 0 Rv2291 sseB Unknown Probable thiosulfate sulfurtransferase SseB Rv2291, (MTCY339.19c), len: 284 aa. Probable sseB, thiosulfate sulfurtransferase. Very similar to thiosulfate sulfurtransferas/rhodanese from Streptomyces coelicolor AL00920 4|SC9B10_21 (283 aa) opt: 765, E(): 0; Smith-Waterman score: 765; 46.9% identity in 286 aa overlap, similar to THTR_ECOLI P31142 putative thiosulfate sulfurtransferase (280 aa), FASTA scores, opt: 478, E(): 1e-23, (35.1% identity in 265 aa overlap) P9WHF5 No intermediary metabolism and respiration Q59570 P9WHF5 GO:0004792,GO:0008272 2.8.1.1 P9WHF5 NC_000962.3 Mycobrowser_v5 CDS 2566772 2567410 . + 0 Rv2295 Rv2295 Unknown Conserved hypothetical protein Rv2295, (MTCY339.15c), len: 212 aa. Conserved hypothetical protein, cysteine-rich protein, similar to YIEJ_ECOLI P31469 hypothetical 22.5 kDa protein in tnab-bglb intergenic region (195 aa), opt: 270, E(): 3.4e-11, (36.4% identity in 198 aa overlap). Alternative start suggested by similarity 26 codons further downstream P9WFL7 No conserved hypotheticals P67309 P9WFL7 NC_000962.3 Mycobrowser_v5 CDS 2567504 2568406 . + 0 Rv2296 Rv2296 Converts haloalkanes to corresponding alcohol and halides [catalytic activity: 1-haloalkane + H2O = a primary alcohol + halide]. Probable haloalkane dehalogenase Rv2296, (MTCY339.14c), len: 300 aa. Probable haloalkane dehalogenase, similar to e.g. HALO_XANAU P22643, haloalkane dehalogenase, (310 aa), opt: 510 z-score: 577.7 E(): 3.1e-25 (39.0% identity in 315 aa overlap). P9WMS3 No intermediary metabolism and respiration P64301 P9WMS3 GO:0018786 3.8.1.5 P9WMS3 NC_000962.3 Mycobrowser_v5 CDS 2568438 2568890 . + 0 Rv2297 Rv2297 Unknown Unknown protein Rv2297, (MTCY339.13c), len: 150 aa. Unknown protein; contains PS00343 Gram-positive cocci surface proteins 'anchoring' hexapeptide P9WLD9 No conserved hypotheticals P9WLD9 P9WLD9 NC_000962.3 Mycobrowser_v5 CDS 2569082 2570053 . + 0 Rv2298 Rv2298 Unknown Conserved protein Rv2298, (MTCY339.12c), len: 323 aa. Conserved protein. Similar to SLR0545 Synechocystis sp, Q55493 hypothetical 34.6 kDa protein (314 aa), FASTA scores, opt: 427, E(): 1.7e-20, (39.3% identity in 303 aa overlap) and to YZAE_BACSU P46905 hypothetical protein in natb 3'region (268 aa) FASTA scores, opt: 370, E(): 6.1e-17, (31.4% identity in 264 aa overlap) P9WQA7 No conserved hypotheticals P63484 P9WQA7 GO:0016491,GO:0055114 1.-.-.- P9WQA7 NC_000962.3 Mycobrowser_v5 CDS 2581843 2582298 . - 0 Rv2309c Rv2309c Use for sequence integration. Integrase is necessary for integration of a phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome. Possible integrase (fragment) Rv2309c, (MTCY3G12.25), len: 151 aa. Possible integrase (fragment), similar to others e.g. Q48908 integrase (fragment) from Mycobacterium paratuberculos (191 aa), FASTA scores: opt: 279, E(): 3.2e-11, (40.4% identity in 136 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. Rv1055|MTV017.08 integrase (fragment) (78 aa) (72.85% identity in 70 aa overlap); and Rv1054|MTV017.07 integrase (fragment). Could belong to the 'phage' integrase family. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P71903 No insertion seqs and phages P71903 P71903 GO:0015074,GO:0006310,GO:0003677 P71903 NC_000962.3 Mycobrowser_v5 CDS 2642150 2642578 . - 0 Rv2360c Rv2360c Unknown Unknown protein Rv2360c, (MTCY27.20), len: 142 aa. Unknown protein. O05838 No conserved hypotheticals O05838 NC_000962.3 Mycobrowser_v5 CDS 2626223 2626519 . - 0 Rv2347c esxP Function unknown Putative ESAT-6 like protein EsxP (ESAT-6 like protein 7) Rv2347c, (MT2412, MTCY98.16c), len: 98 aa. EsxP, ESAT-6 like protein (see citation below). Member of M. tuberculosis hypothetical QILSS protein family with Rv1197, Rv1792, Rv1038c and Rv3620c. Belongs to the ESAT6 family. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WNI5 No cell wall and cell processes 3OGI P9WNI5 P9WNI5 NC_000962.3 Mycobrowser_v5 CDS 2573015 2573707 . + 0 Rv2301 cut2 Hydrolysis of cutin (a polyester that forms the structure of plant cuticle). Probable cutinase Cut2 Rv2301, (MTCY339.08c), len: 230 aa. Probable cut2 (alternate gene name: cfp25), cutinase, highly similar to others from Mycobacteria tuberculosis e.g. MTCY13E12.04|Rv3451|O06318|CUT3_MYCTU (247 aa), FASTA scores: opt: 569, E(): 2.3e-27, (45.3% identity in 223 aa overlap); MT2037|MTCY39.35|RV1984C|Q10837|CUT1_MYCTU (217 aa), FASTA scores: opt: 383, E(): 3.4e-16 (42.9% identity in 217 aa overlap); O69691|Rv3724|MTV025.072 putative cutinase precursor (187 aa), FASTA scores: opt: 248, E(): 4.3e-08, (41.85% identity in 172 aa overlap); etc. Also similar to few others from other organisms e.g. Q9KK87 serine esterase cutinase from Mycobacterium avium (220 aa), FASTA scores: opt: 391, E(): 1.1e-16, (39.15% identity in 235 aa overlap); etc. Contains PS00095 C-5 cytosine-specific DNA methylases C-terminal signature. Belongs to the cutinase family. Start changed since first submission (+11 aa). P9WP41 No cell wall and cell processes P63881 P9WP41 GO:0005576,GO:0008152,GO:0050525 3.1.1.74 P9WP41 NC_000962.3 Mycobrowser_v5 CDS 2573813 2574055 . + 0 Rv2302 Rv2302 Function unknown Conserved protein Rv2302, (MTCY339.07c), len: 80 aa. Conserved protein, highly similar to others: O53766|AL021942|Rv0569|MTV039.07 hypothetical 9.5 KDA protein from Mycobacterium tuberculosis (88 aa), FASTA scores: opt: 300, E(): 1.4e-14, (61.85% identity in 76 aa overlap); O88049|SCI35.11 hypothetical 7.1 KDA protein from Streptomyces coelicolor (64 aa), FASTA scores: opt: 169, E(): 1.5e-05, (46.55% identity in 58 aa overlap) (has its C-terminus shorter); Q9XCD1 hypothetical 12.0 KDA protein (fragment) from Thermomonospora fusca (106 aa), FASTA scores: opt: 126, E(): 0.023, (50.0% identity in 34 aa overlap) (similarity in part for this one). Also weakly similar to U650M|G699303|Q50105 hypothetical 5.7 KDA protein from Mycobacterium leprae (53 aa), FASTA scores: opt: 89, E(): 0.66, (45.5% identity in 33 aa overlap); and weakly similar to N-terminus of Q9RIZ1|SCJ1.23c putative DNA-binding protein from Streptomyces coelicolor (323 aa), FASTA scores: opt: 182, E(): 7.3e-06, (42.25% identity in 71 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLD5 No conserved hypotheticals 2A7Y P64983 P9WLD5 P9WLD5 NC_000962.3 Mycobrowser_v5 CDS 2583045 2583332 . + 0 Rv2309A Rv2309A Unknown Hypothetical protein Rv2309A, len: 95 aa. Hypothetical unknown protein. Equivalent to AAK46663 from Mycobacterium tuberculosis strain CDC1551 (95 aa) but longer 13 aa. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L7N666 No conserved hypotheticals L7N666 L7N666 NC_000962.3 Mycobrowser_v5 CDS 2599988 2600479 . + 0 Rv2327 Rv2327 Function unknown Conserved protein Rv2327, (MTCY3G12.07c), len: 163 aa. Conserved protein, similar to Z80775|MTCY21D4.05c|Rv0042c from Mycobacterium tuberculosis (208 aa), FASTA scores: opt: 242, E(): 5e-08, (43.0% identity in 107 aa overlap). Also slight similarity to putative transcriptional regulatory proteins belonging to the MarR-family e.g. Q9CCY2/ML2696 from Mycobacterium leprae (243 aa), FASTA scores: opt: 245, E(): 3.7e-08, (35.35% identity in 150 aa overlap); Q9L135|SC6D11.20 from Streptomyces coelicolor (155 aa), FASTA scores: opt: 242, E(): 3.9e-08, (34.75% identity in 141 aa overlap); etc. P71885 No conserved hypotheticals P71885 P71885 GO:0006355,GO:0006350,GO:0003700,GO:0005622 P71885 NC_000962.3 Mycobrowser_v5 CDS 2867783 2868061 . + 0 Rv2545 vapB18 Unknown Possible antitoxin VapB18 Rv2545, (MTY159.11c), len: 92 aa. Possible vapB18, antitoxin, part of toxin-antitoxin (TA) operon with Rv2546 (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. O33300|Rv2758c|MTV002.23c (88 aa), FASTA scores: opt: 151, E(): 9.8e-05, (66.65% identity in 45 aa overlap); and Q10771|Rv1560|MT1611|MTCY48.05 (72 aa), FASTA scores: opt: 84, E(): 8.2, (46.5% identity in 43 aa overlap). P9WJ47 No virulence, detoxification, adaptation P95008 P9WJ47 P9WJ47 NC_000962.3 Mycobrowser_v5 CDS 2575016 2575225 . - 0 Rv2304c Rv2304c Unknown Hypothetical protein Rv2304c, (MTCY339.05), len: 69 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLD3 No conserved hypotheticals P9WLD3 NC_000962.3 Mycobrowser_v5 tRNA 2581764 2581837 . - 0 MTB000028 metV - tRNA-Met (CAT) metV, tRNA-Met, anticodon cat, length = 74. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 2575809 2577098 . + 0 Rv2305 Rv2305 Unknown Unknown protein Rv2305, (MTCY339.04c), len: 429 aa. Unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLD1 No conserved hypotheticals Q50660 P9WLD1 NC_000962.3 Mycobrowser_v5 CDS 2577488 2577922 . + 0 Rv2306B Rv2306B Unknown Possible conserved membrane protein Rv2306B, len: 144 aa. Possible conserved membrane protein, similar to C-terminal part of several hypothetical membrane proteins from Mycobacterium tuberculosis and Streptomyces coelicolor e.g. P96915|Y625_MYCTU|RV0625c hypothetical 25.2 KDA protein from Mycobacterium tuberculosis (246 aa), FASTA scores: opt: 480, E(): 5e-24, (77.15% identity in 92 aa overlap). Could be a continuation of Rv2306A suggesting there may be a frameshift near nt 2577473. The C-terminal part is longer than Rv0625c and the 3'-end of gene overlaps Rv2307c, so maybe a further framehift. However, sequence has been checked and no error found. Also same sequence as strain CDC1551 and Mycobacterium bovis. Replaces original Rv2306c on other strand. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q79FG4 No cell wall and cell processes Q79FG4 Q79FG4 NC_000962.3 Mycobrowser_v5 CDS 2577851 2578696 . - 0 Rv2307c Rv2307c Unknown Conserved hypothetical protein Rv2307c, (MTCY339.02), len: 281 aa. Conserved hypothetical protein, similar to many other hypothetical proteins and BEM1/BUD5 suppressors e.g. P77538 hypothetical protein from Escherichia coli (293 aa), FASTA scores: opt: 421, E(): 2.4e-18, (32.1% identity in 268 aa overlap) (alias AAG57647|Z3802|BAB36823|ECS3400 Putative enzyme (3.4.-) from Escherichia coli (293 aa), FASTA scores: opt: 425, E(): 1.7e-18, (32.1% identity in 268 aa overlap));P54069|BE46_SCHPO|BEM46|SPBC32H8.03|PI020 BEM46 protein from Schizosaccharomyces pombe (Fission yeast) (352 aa), FASTA scores: opt: 355, E(): 3.3e-14, (30.45% identity in 279 aa overlap); O76462|BEM46 BEM46 protein from Drosophila melanogaster (338 aa), FASTA scores: opt: 404, E(): 2.8e-17, (32.75% identity in 281 aa overlap); etc. Equivalent (but with few differences) to AAK46650|MT2364 protein from Mycobacterium tuberculosis strain CDC1551 (281 aa). Predicted to be an outer membrane protein (See Song et al., 2008). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLC7 No conserved hypotheticals Q50658 P9WLC7 GO:0016021,GO:0005886,GO:0016787 P9WLC7 NC_000962.3 Mycobrowser_v5 CDS 2579228 2579419 . - 0 Rv2307A Rv2307A Unknown Hypothetical glycine rich protein Rv2307A, len: 63 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L7N678 No conserved hypotheticals L7N678 NC_000962.3 Mycobrowser_v5 CDS 2580419 2581135 . + 0 Rv2308 Rv2308 Function unknown Conserved hypothetical protein Rv2308, (MTCY339.01c), len: 238 aa. Conserved hypothetical protein, sharing similarity with O53464|Rv2018|MTV018.05 from Mycobacterium tuberculosis (239 aa), FASTA scores: opt: 142, E(): 0.034, (24.8% identity in 250 aa overlap). As contains possible helix-turn-helix motif at aa 16-37 (Sequence: YVYAEVDKLIGLPAGTAKRWIN) (Score 1169, +3.17 SD), may be a transcriptional regulator. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLC5 No conserved hypotheticals Q50657 P9WLC5 P9WLC5 NC_000962.3 Mycobrowser_v5 CDS 2583884 2584408 . + 0 Rv2311 Rv2311 Function unknown Conserved hypothetical protein Rv2311, (MTCY3G12.23c), len: 174 aa. Conserved hypothetical protein, with similarity (in part) to transfer proteins homologous TRAA e.g. Q9EUN8|TRAA transfer protein homolog TRAA from Corynebacterium glutamicum (1160 aa), FASTA scores: opt: 221, E(): 2.9e-07, (36.8% identity in 136 aa overlap); Q9ETQ3|TRAA conjugal transfer protein (TRAA-like protein) from Corynebacterium equii (1367 aa), FASTA scores: opt: 188, E(): 5.5e-05, (33% identity in 106 aa overlap); P55418|TRAA_RHISN|Y4DS probable conjugal transfer protein from Rhizobium sp. strain NGR234 (1102 aa), FASTA scores: opt: 145, E(): 0.035, (29.08% identity in 141 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLC1 No conserved hypotheticals P9WLC1 P9WLC1 NC_000962.3 Mycobrowser_v5 CDS 2585917 2587290 . - 0 Rv2314c Rv2314c Function unknown Conserved protein Rv2314c, (MTCY3G12.20), len: 457 aa. Conserved protein, highly similar to Q9RJ51|SCI8.02 hypothetical protein from Streptomyces coelicolor (464 aa) FASTA scores: opt: 1485, E(): 5.2e-83, (53.5% identity in 454 aa overlap); similar to AAK24788|CC2824 TldD/PmbA family protein from Caulobacter crescentus (441 aa), FASTA scores: opt: 364, E(): 8.3e-15, (29.8% identity in 460 aa overlap); and showing similarity with Q9HJZ6|TA0814 hypothetical protein from Thermoplasma acidophilum (430 aa), FASTA scores: opt: 220, E(): 4.7e-06, (21.85% identity in 348 aa overlap). P71898 No conserved hypotheticals P71898 P71898 NC_000962.3 Mycobrowser_v5 CDS 2596334 2596780 . + 0 Rv2324 Rv2324 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably AsnC-family) Rv2324, (MTCY3G12.10), len: 148 aa. Probable transcriptional regulatory protein, asnC-family, similar to other putative AsnC-family regulatory proteins e.g. Q9L101|SCL6.15C from Streptomyces coelicolor (150 aa) FASTA scores: opt: 466, E(): 2.4e-24, (52.8% identity in 142 aa overlap); Q9RKY4|SC6D7.14 putative AsnC-family transcriptional regulatory protein from Streptomyces coelicolor (165 aa), FASTA scores: opt: 266, E(): 5.5e-11, (32.4% identity in 145 aa overlap); Q9ZEP1|LRPA|SCE94.12c putative transcriptional regulator from Streptomyces coelicolor (150 aa), FASTA scores: opt: 249, E(): 6.9e-10, (33.35% identity in 147 aa overlap); etc. Also similar to P96896|Rv3291c|MTCY71.31c from Mycobacterium tuberculosis (150 aa), FASTA scores: opt: 261, E(): 1.1e-10, (36.4% identity in 143 aa overlap). P71888 No regulatory proteins P71888 P71888 GO:0006355,GO:0043565,GO:0006350,GO:0003700,GO:0005622 P71888 NC_000962.3 Mycobrowser_v5 CDS 2600731 2601879 . + 0 Rv2328 PE23 Function unknown PE family protein PE23 Rv2328, (MTCY3G12.06), len: 382 aa. PE23, Member of the Mycobacterium tuberculosis PE family (see citation below), similar to others e.g. Q9L8K5|MAG24-1 PE-PGRS homolog from Mycobacterium marinum (638 aa), FASTA scores: opt: 495, E(): 6.6e-18, (34.65% identity in 401 aa overlap); etc. P9WIG9 No PE/PPE P0A684 P9WIG9 GO:0005886,GO:0016021 P9WIG9 NC_000962.3 Mycobrowser_v5 CDS 2611869 2612987 . - 0 Rv2337c Rv2337c Unknown Hypothetical protein Rv2337c, (MTCY98.06c), len: 372 aa. Hypothetical unknown protein, sharing some similarity with Q9RI33|SCJ12.27c hypothetical 37.2 KDA protein from Streptomyces coelicolor (335 aa), blast scores: 134 and 46, (28% and 33% identity, 52% and 44% positive); FASTA scores: opt: 176, E(): 0.00042, (31.95% identity in 355 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P95233 No conserved hypotheticals P95233 P95233 NC_000962.3 Mycobrowser_v5 CDS 2958909 2959190 . - 0 Rv2632c Rv2632c Function unknown Conserved protein Rv2632c, (MTCY441.02c), len: 93 aa. Conserved protein, highly similar to conserved hypothetical proteins from Mycobacterium tuberculosis: P71996|YH38_MYCTU|Rv1738|MT1780|MTCY04C12.23 (94 aa), FASTA scores: opt: 319, E(): 4.2e-15, (53.95% identity in 89 aa overlap); and Q9KK61 from Mycobacterium bovis BCG (56 aa), FASTA scores: opt: 178, E(): 9.2e-06, (52.95% identity in 51 aa overlap). P9WL61 No conserved hypotheticals 2FGG P65033 P9WL61 P9WL61 NC_000962.3 Mycobrowser_v5 CDS 2646747 2647088 . - 0 Rv2365c Rv2365c Function unknown Conserved hypothetical protein Rv2365c, (MTCY27.15), len: 113 aa. Conserved hypothetical protein, highly similar to Q49767|ML0630|B1937_F3_101|CAC30138 Hypothetical protein from Mycobacterium leprae (108 aa), FASTA scores: opt: 426, E(): 1.4e-18, (67.9% identity in 106 aa overlap). Also highly similar to Q9RDF3|SCC77.05 from Streptomyces coelicolor (132 aa), FASTA scores: opt: 254, E(): 1.9e-18, (53.1% identity in 96 aa overlap). Equivalent to AAK46728 from Mycobacterium tuberculosis strain CDC1551 (93 aa) but longer 20 aa. O05833 No conserved hypotheticals O05833 NC_000962.3 Mycobrowser_v5 CDS 2589697 2590521 . + 0 Rv2317 uspB Thought to be involved in active transport of sugar across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable sugar-transport integral membrane protein ABC transporter UspB Rv2317, (MTC3G12.17c), len: 274 aa. Probable uspB, sugar-transport integral membrane protein ABC transporter (see citation below), most similar to Q9CBN7|USPE|ML1769 sugar transport integral membrane protein from Mycobacterium leprae (274 aa), FASTA scores: opt: 1522, E(): 3.4e-89, (85.0% identity in 274 aa overlap); and similar to O32941|ML1425|MLCB2052.29 probable ABC-transport protein, inner membrane component from Mycobacterium leprae (283 aa), FASTA scores: opt: 630, E(): 8.4e-33, (36.55% identity in 268 aa overlap). Also similar to other integral membrane proteins e.g. P73854|LACG|SLR1723 lactose transport system permease protein from Synechocystis sp. strain PCC 6803 (270 aa), FASTA scores: opt: 605, E(): 3.1e-31, (36.0% identity in 264 aa overlap); Q9F3B8|SC5F1.11 putative sugar transport integral membrane protein from Streptomyces coelicolor (307 aa), FASTA scores: opt: 582, E(): 9.7e-30, (34.45% identity in 264 aa overlap); etc. Also similar to O53483|Rv2039c|MTV018.26c sugar transport protein from Mycobacterium tuberculosis (280 aa), FASTA scores: opt: 630, E(): 8.3e-89, (37.7% identity in 268 aa overlap). L7N652 No cell wall and cell processes Q7D7B8 L7N652 GO:0005886,GO:0006810,GO:0005215,GO:0016021 L7N652 NC_000962.3 Mycobrowser_v5 CDS 2590518 2591840 . + 0 Rv2318 uspC Thought to be involved in active transport of sugar across the membrane (import). Probable periplasmic sugar-binding lipoprotein UspC Rv2318, (MTCY3G12.16c), len: 440 aa. Probable uspC, sugar-binding lipoprotein component of sugar transport system (see citation below), most similar to Q9CBN6|USPC|ML1770 sugar transport periplasmic binding protein from Mycobacterium leprae (446 aa), FASTA scores: opt: 2294, E(): 8.1e-135, (74.7% identity in 446 aa overlap). Also similar to other substrate-binding proteins e.g. Q9RK89|SCF1.15 putative substrate binding protein (extracellular) (binding-protein-dependent transport) (fragment) from Streptomyces coelicolor (221 aa), FASTA scores: opt: 377, E(): 3e-16, (32.25% identity in 217 aa overlap); Q9K6N8|BH3690 sugar transport system (sugar-binding protein) from Bacillus halodurans (420 aa), FASTA scores: opt: 227, E(): 1e-06, (25.00% identity in 452 aa overlap); etc. Also similar to O53485|Rv2041c|MTV018.28C lipoprotein component of sugar transport system from Mycobacterium tuberculosis (439 aa), FASTA scores: opt: 246, E(): 7e-08, (26.75% identity in 325 aa overlap). Contains a hydrophobic stretch (possible signal peptide) at N-terminal end. P71894 No cell wall and cell processes P71894 P71894 GO:0005215,GO:0006810 P71894 NC_000962.3 Mycobrowser_v5 CDS 2592723 2594153 . - 0 Rv2320c rocE Thought to be involved in transport of cationic amino acid (especially arginine and ornithine) across the membrane. Responsible for the translocation of the substrate across the membrane. Probable cationic amino acid transport integral membrane protein RocE Rv2320c, (MTCY3G12.14), len: 476 aa. Probable rocE, cationic amino acid (especially arginine and ornithine) transporter (permease), highly similar to other amino acid transporters e.g. Q9L100|SCL6.16C putative amino acid transporter from Streptomyces coelicolor (496 aa), FASTA scores: opt: 1485, E(): 9.4e-82, (48.4% identity in 477 aa overlap); O06479|YFNA putative amino acid transporter from Bacillus subtilis (462 aa), FASTA scores: opt: 1271, E(): 6.1e-69, (41.9% identity in 463 aa overlap); Q9PG94|XF0408 amino acid transporter from Xylella fastidiosa (509 aa), FASTA scores: opt: 1128, E(): 2.5e-60, (39.5% identity in 481 aa overlap); etc. Also some similarity with Z99108.1|BSUB0005 from Bacillus subtilis (461 aa), FASTA scores: opt: 1271, E(): 0, (41.9% identity in 463 aa overlap); and G403170 ethanolamine permease (488 aa), FASTA scores: opt: 468, E(): 1e-23, (28.1% identity in 462 aa overlap). Seems to belong to the APC family. P71892 No cell wall and cell processes P71892 P71892 GO:0006865,GO:0016021,GO:0055085,GO:0015171 P71892 NC_000962.3 Mycobrowser_v5 tRNA 2619407 2619479 . - 0 MTB000029 asnT - tRNA-Asn (GTT) asnT, tRNA-Asn, anticodon gtt, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 2594699 2595364 . - 0 Rv2322c rocD1 Involved in arginine metabolism [catalytic activity: L-ornithine + a 2-oxo acid = L-glutamate 5-semialdehyde + an L-amino acid]. Probable ornithine aminotransferase (N-terminus part) RocD1 (ornithine--oxo-acid aminotransferase) Rv2322c, (MTCY3G12.12), len: 221 aa. Probable rocD1, ornithine aminotransferase, highly similar to N-terminal region of other ornithine aminotransferases, e.g. Q9FC90|ROCD from Streptomyces coelicolor (407 aa), FASTA scores: opt: 770, E(): 8.7e-40, (55.7% identity in 201 aa overlap); BAB42057|ROCD|SA0818 from Staphylococcus aureus subsp. aureus N315 (396 aa) FASTA scores: opt: 632, E(): 2.2e-31, (46.1% identity in 208 aa overlap); P38021|OAT_BACSU|ROCD from Bacillus subtilis (401 aa), FASTA scores: opt: 626, E(): 5.1e-31, (43.1% identity in 218 aa overlap); etc. Belongs to class-III of pyridoxal-phosphate-dependent aminotransferases. Rv2322c|MTCY3G12.12 and Rv2321c|MTCY3G12.13 (upstream ORF) appear to be an ornithine aminotransferase homologue but are frameshifted - we can find no sequence error in the cosmid to account for this. P71890 No intermediary metabolism and respiration P71890 P71890 GO:0030170,GO:0004587 2.6.1.13 P71890 NC_000962.3 Mycobrowser_v5 CDS 2698042 2698245 . - 0 Rv2401A Rv2401A Unknown Possible conserved membrane protein Rv2401A, len: 67 aa. Possible conserved membrane protein, highly similar, but with 29 aa shorter, to ML0614|AL583919_34|Q49760 from Mycobacterium leprae (95 aa), FASTA scores: opt: 297, E(): 3.6e-15, (67.7% identity in 65 aa overlap). Has hydrophobic stretch. Q79FE4 No cell wall and cell processes Q79FE4 NC_000962.3 Mycobrowser_v5 CDS 2595361 2596269 . - 0 Rv2323c Rv2323c Function unknown Conserved protein Rv2323c, (MTCY3G12.11), len: 302 aa. Conserved protein, highly similar to others e.g. Q9FC91|2SCG58.22 conserved hypothetical protein from Streptomyces coelicolor (288 aa), FASTA scores: opt: 561, E(): 7.3e-28, (46.95% identity in 279 aa overlap); P74535|SLL1336 hypothetical 78.3 KDA protein from Synechocystis sp. (705 aa), FASTA scores: opt: 555, E(): 2.1e-27, (37.75% identity in 265 aa overlap); etc. Also similar to various hydrolases e.g. Q53797 beta-hydroxylase (bleomycin/phleomycin binding protein, ankyrin homologue, bleomycin and transport protein) from Streptomyces verticillus (326 aa), FASTA scores: opt: 211, E(): 4.5e-06, (26.75% identity in 303 aa overlap); Q9X7M4|DDAH_STRCO|SC5F2A.01c NG,NG-dimethylarginine dimethylaminohydrolase (Dimethylargininase) (Dimethylarginine dimethylaminohydrolase) (258 aa), FASTA scores: opt: 209, E(): 4.9e-06, (27.15% identity in 243 aa overlap); G434715 beta-hydroxylase (bleomicin/phleomycin binding protein) from Streptomyces verticillus (326 aa), FASTA scores: opt: 211, E(): 4.5e-06, (26.75% identity in 303 aa overlap); etc. P71889 No conserved hypotheticals P71889 P71889 GO:0016813,GO:0005737 P71889 NC_000962.3 Mycobrowser_v5 CDS 2610837 2611805 . + 0 Rv2336 Rv2336 Unknown. May be involved in virulence. Hypothetical protein Rv2336, (MTCY98.05), len: 322 aa. Hypothetical unknown protein (see Rindi et al., 2001). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P95232 No conserved hypotheticals P95232 NC_000962.3 Mycobrowser_v5 CDS 2617667 2618908 . - 0 Rv2340c PE_PGRS39 Function unknown PE-PGRS family protein PE_PGRS39 Rv2340c, (MTCY98.09c), len: 413 aa. PE_PGRS39, Member of the Mycobacterium tuberculosis PE_family, PGRS subfamily of gly-rich proteins (see citations below), similar to others eg YI18_MYCTU|Q50615|Rv1818c|MTCY1A11.25 PE-PGRS family protein from Mycobacterium tuberculosis (498 aa), FASTA scores: opt: 710, E(): 1.4e-22, (41.0% identity in 368 aa overlap); O53884|Rv0872v|MTV043.65c PGRS-family protein from Mycobacterium tuberculosis (606 aa), FASTA scores: opt: 708, E(): 1.9e-22, (42.4% identity in 389 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L7N659 No PE/PPE Q7D7A7 L7N659 L7N659 NC_000962.3 Mycobrowser_v5 CDS 2683709 2684266 . - 0 Rv2390c Rv2390c Function unknown Conserved hypothetical protein Rv2390c, (MTCY253.31), len: 185 aa. Conserved hypothetical protein, similar to other Mycobacterium tuberculosis proteins Q11032|YD62_MYCTU|MTCY02B10.26c|Rv1362c hypothetical 23.5 kDa protein (220 aa), FASTA scores: opt: 223, E(): 2.1e-07, (27.4% identity in 190 aa overlap); and Q11033|YD63_MYCTU|MTCY02B10.27c|Rv1363c hypothetical 28.3 kDa protein (261 aa), FASTA scores: opt: 238, E(): 2.7e-08, (27.6% identity in 163 aa overlap). P71754 No conserved hypotheticals P71754 P71754 NC_000962.3 Mycobrowser_v5 ncRNA 2692172 2692521 . + 0 MTB000067 mcr7 Unknown Putative small regulatory RNA mcr7, putative small regulatory RNA (See DiChiara et al., 2010). 5'-end mapped by 5'RLM-RACE in M. bovis BGC Pasteur, 3'-end not mapped. No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 2601914 2603461 . - 0 Rv2329c narK1 Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate. Probable nitrite extrusion protein 1 NarK1 (nitrite facilitator 1) Rv2329c, (MTCY3G12.05), len: 515 aa. Probable narK1, nitrite extrusion protein, possibly member of major facilitator superfamily (MFS). Equivalent to O32974|MLCB22.41c|nark|ML0844 putative nitrite extrusion protein from Mycobacterium leprae (517 aa), FASTA scores: opt: 2224, E(): 1.9e-129, (69.3% identity in 488 aa overlap). Also highly similar to others e.g. P94933 nitrite extrusion protein from Mycobacterium fortuitum (471 aa), FASTA scores: opt: 1969, E(): 8.6e-114, (62.1% identity in 459 aa overlap); P37758|NARU_ECOLI nitrite extrusion protein 2 from Escherichia coli strain K12 (462 aa), FASTA scores: opt: 792, E(): 2.3e-41, (36.95% identity in 476 aa overlap); P10903|NARK_ECOLI nitrite extrusion protein (nitrite facilitator 1) from Escherichia coli strain K12 (463 aa), FASTA scores: opt: 784, E(): 7e-41, (35.3% identity in 468 aa overlap); etc. Also similar to RV0261c|Z86089|MTCY6A4_5 from Mycobacterium tuberculosis (469 aa), FASTA scores: opt: 2000, E(): 1.1e-115, (62.6% identity in 470 aa overlap). Belongs to the nark/NASA family of transporters. P71883 No cell wall and cell processes P71883 P71883 GO:0055085 NC_000962.3 Mycobrowser_v5 CDS 2603695 2604222 . - 0 Rv2330c lppP Unknown Probable lipoprotein LppP Rv2330c, (MTCY3G12.04), len: 175 aa. Probable lppP, lipoprotein. Contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. P9WK69 No cell wall and cell processes P9WK69 GO:0005886 NC_000962.3 Mycobrowser_v5 CDS 2604297 2604683 . + 0 Rv2331 Rv2331 Unknown Hypothetical protein Rv2331, (MT2393, MTCY3G12.03c), len: 128 aa. Hypothetical unknown protein; shortened version of MTCY3G12.03c to eliminate overlap with MTCY3G12.04. P9WLB3 No conserved hypotheticals P9WLB3 NC_000962.3 Mycobrowser_v5 CDS 2604740 2605078 . + 0 Rv2331A Rv2331A Unknown Hypothetical protein Rv2331A, len: 112 aa. Hypothetical unknown protein. Q79FF7 No conserved hypotheticals Q79FF7 Q79FF7 NC_000962.3 Mycobrowser_v5 CDS 2606708 2608321 . - 0 Rv2333c stp Involved in transport of drug across the membrane (export) Integral membrane drug efflux protein Stp Rv2333c, (MTCY3G12.01), len: 537 aa. stp, integral membrane drug efflux protein (See Ramon-Garcia et al., 2007), member of major facilitator superfamily (MFS), highly similar to many e.g. Q9RL22|C5G9.04c putative transmembrane efflux protein from Streptomyces coelicolor (489 aa), FASTA scores: opt: 1031, E(): 4e-55, (37.4% identity in 412 aa overlap); Q9L0L9|SCD82.12 putative transmembrane efflux protein from Streptomyces coelicolor (490 aa), FASTA scores: opt: 883, E(): 3.8e-46, (36.35% identity in 407 aa overlap); Q9ZBW5|SC4B5.03c putative integral membrane efflux protein from Streptomyces coelicolor (504 aa), FASTA scores: opt: 899, E(): 4.1e-47, (37.4% identity in 415 aa overlap); P39886|TCMA_STRGA tetracenomycin C resistance and export protein from Streptomyces glaucescens (538 aa), FASTA scores: opt: 839, E(): 1.9e-43, (32.3% identity in 489 aa overlap); etc. Also highly similar to Rv2459|O53186|MTV008.15 probable conserved integral membrane transport protein from Mycobacterium tuberculosis strain H37Rv (508 aa), FASTA scores: opt: 1385, E(): 1.5e-76, (44.05% identity in 504 aa overlap); and AAK46834|MT2534 drug transporter from Mycobacterium tuberculosis strain CDC1551 (523 aa), FASTA scores: opt: 1385, E(): 1.5e-76, (44.4% identity in 504 aa overlap). P9WG91 No cell wall and cell processes P71879 P9WG91 GO:0005886,GO:0046677,GO:0015904,GO:0015520,GO:0055085,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2609732 2610421 . + 0 Rv2335 cysE Involved in cysteine biosynthesis [catalytic activity: acetyl-CoA + L-serine = CoA + O-acetyl-L-serine]. Probable serine acetyltransferase CysE (sat) Rv2335, (MTCY98.04), len: 229 aa. Probable cysE, serine acetyltransferase, equivalent to O32979|CYSE|ML0838 serine acetyltransferase from Mycobacterium leprae (227 aa), FASTA scores: opt: 1152, E(): 9.6e-62, (76.4% identity in 229 aa overlap). Also highly similar, except in C-terminal part, to others e.g. Q9HXI6|CYSE|PA3816 O-acetylserine synthase from Pseudomonas aeruginosa (258 aa), FASTA scores: opt: 737, E(): 6e-37, (61.3% identity in 168 aa overlap); P23145|NIFP_AZOCH probable serine acetyltransferase from Azotobacter chroococcum mcd 1 (269 aa), FASTA scores: opt: 718, E(): 8.4e-36, (55.45% identity in 220 aa overlap); Q06750|CYSE_BACSU serine acetyltransferase from Bacillus subtilis (217 aa), FASTA scores: opt: 640, E(): 3.1e-31, (48.0% identity in 200 aa overlap); etc. Contains PS00101 Bacterial hexapeptide-repeat containing-transferases signature. Belongs to the CYSE/LACA/LPXA/NODL family of acetyltransferases. Composed of multiple repeats of [LIV]-G-X(4). P95231 No intermediary metabolism and respiration P95231 GO:0005737,GO:0009001,GO:0006535 2.3.1.30 P95231 NC_000962.3 Mycobrowser_v5 CDS 2614693 2617581 . + 0 Rv2339 mmpL9 Unknown. Thought to be involved in fatty acid transport. Probable conserved transmembrane transport protein MmpL9 Rv2339, (MTCY98.08), len: 962 aa. Probable mmpL9, conserved transmembrane transport protein (see citation below), with strong similarity to other Mycobacterial proteins e.g. P54881|YV34_MYCLE|MML4_MYCLE hypothetical 105.2 kDa protein from Mycobacterium leprae (959 aa), FASTA scores: opt: 3799, E(): 0, (59.3% identity in 937 aa overlap); G699237|U1740AB from Mycobacterium leprae; and MTCY20G9.34; MTCY48.08c; MTCY19G5.06 from Mycobacterium tuberculosis. Belongs to the MmpL family. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WJU3 No cell wall and cell processes P95235 P9WJU3 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2619597 2620016 . + 0 Rv2341 lppQ Unknown Probable conserved lipoprotein LppQ Rv2341, (MTCY98.10), len: 139 aa. Probable lppQ, conserved lipoprotein, showing some similarity with Rv1228|O33224|LPQX|MTCI61.11 from Mycobacterium tuberculosis (185 aa), FASTA scores: opt: 155; E(): 0.0073; (31.9% identity in 116 aa overlap). Also shows few similarity with P29228|VLPA_MYCHR variant surface antigen a precursor from Mycoplasma hyorhinis (157 aa), FASTA scores: opt: 96, E(): 7.3, (23.1% identity in 143 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. P95237 No cell wall and cell processes P95237 NC_000962.3 Mycobrowser_v5 CDS 2620272 2620529 . + 0 Rv2342 Rv2342 Function unknown Conserved hypothetical protein Rv2342, (MTCY98.11), len: 85 aa. Conserved hypothetical protein, highly similar to Q9CCG1|ML0834 hypothetical protein from Mycobacterium leprae (100 aa), FASTA scores: opt: 392, E(): 2.9e-20, (78.2% identity in 78 aa overlap). N-terminus highly similar to N-terminal part of Q9L085|SCC24.32 putative secreted protein from Streptomyces coelicolor (108 aa), FASTA scores: opt: 122, E(): 0.077, (39.15% identity in 46 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P95238 No conserved hypotheticals P95238 NC_000962.3 Mycobrowser_v5 CDS 2635903 2636889 . + 0 Rv2355 Rv2355 Required for the transposition of the insertion sequence IS6110. Probable transposase Rv2355, (MTCY98.24), len: 328 aa. Probable IS6110 transposase. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2354 and Rv2355, the sequence UUUUAAAG (directly upstream of Rv2355) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 16 aa). P9WKH9 No insertion seqs and phages P0C5G8 P9WKH9 GO:0015074,GO:0006310,GO:0032196,GO:0003677 P9WKH9 NC_000962.3 Mycobrowser_v5 CDS 2728437 2728847 . - 0 Rv2432c Rv2432c Unknown Hypothetical protein Rv2432c, (MTCY428.14), len: 136 aa. Hypothetical unknown protein. P71917 No conserved hypotheticals P71917 P71917 NC_000962.3 Mycobrowser_v5 CDS 2947096 2947449 . - 0 Rv2619c Rv2619c Function unknown Conserved protein Rv2619c, (MTCY01A10.14), len: 117 aa. Conserved protein, highly similar to Q9L0F3|SCD31.14 hypothetical 11.6 KDA protein from Streptomyces coelicolor (110 aa), FASTA scores: opt: 407, E(): 2.3e-21, (55.95% identity in 109 aa overlap). Also similarity with other short bacterial hypothetical proteins e.g. Q9F8B9 hypothetical 12.4 KDA protein from Streptococcus agalactiae (112 aa), FASTA scores: opt: 143, E(): 0.0032, (32.45% identity in 74 aa overlap); etc. O06194 No conserved hypotheticals O06194 O06194 O06194 NC_000962.3 Mycobrowser_v5 CDS 2959335 2959820 . - 0 Rv2633c Rv2633c Unknown Hypothetical protein Rv2633c, (MTCY441.03c), len: 161 aa. Hypothetical unknown protein. P9WL59 No conserved hypotheticals P65035 P9WL59 P9WL59 NC_000962.3 Mycobrowser_v5 CDS 2622457 2623752 . - 0 Rv2344c dgt dGTPase preferentially hydrolyzes dGTP over the other canonical NTPS [catalytic activity: dGTP + H(2)O = deoxyguanosine + triphosphate]. Probable deoxyguanosine triphosphate triphosphohydrolase Dgt (dGTPase) (dGTP triphosphohydrolase) Rv2344c, (MT2409, MTCY98.13c), len: 431 aa. Probable dgt, deoxyguanosine triphosphate triphosphohydrolase, equivalent to Q9CCG3|DGT|ML0831 putative deoxyguanosine triphosphate triphosphohydrolase from Mycobacterium leprae (429 aa), FASTA scores: opt: 2316, E(): 1.6e-137, (83.85% identity in 421 aa overlap); and O52199|DGTP_MYCSM|AF027507_2 deoxyguanosinetriphosphate triphosphohydrolase from Mycobacterium smegmatis (428 aa), FASTA scores: opt: 1991, E(): 3.4e-117, (73.5% identity in 422 aa overlap). Also highly similar or similar to several deoxyguanosine triphosphate hydrolases e.g. Q9L2E9|SC7A8.09c putative deoxyguanosinetriphosphate triphosphohydrolase from Streptomyces coelicolor (424 aa), FASTA scores: opt: 1216, E(): 1e-68, (51.05% identity in 425 aa overlap); BAB48544|MLL1093 dGTP triphosphohydrolase from Rhizobium loti (Mesorhizobium loti) (404 aa), FASTA scores: opt: 489, E(): 3.1e-23, (33.85% identity in 387 aa overlap); P15723|DGTP_ECOLI|DGT|B0160 from Escherichia coli strain K12 (504 aa), FASTA scores: opt: 173, E(): 0.0022, (31.65% identity in 259 aa overlap); etc. Belongs to the dGTPase family. P9WNY7 No intermediary metabolism and respiration P0A540 P9WNY7 GO:0008832,GO:0000287,GO:0046039 P9WNY7 NC_000962.3 Mycobrowser_v5 CDS 2623821 2625803 . + 0 Rv2345 Rv2345 Unknown Possible conserved transmembrane protein Rv2345, (MTCY98.14), len: 660 aa. Possible conserved transmembrane protein, with hydrophobic stretch at N-terminal end around position 180. Similar to O52198 hypothetical 21.2 KDA protein (fragment) from Mycobacterium smegmatis (195 aa), FASTA scores: opt: 589, E(): 1.5e-23; (47.2% identity in 195 aa overlap). P9WFJ5 No cell wall and cell processes P95241 P9WFJ5 GO:0005886,GO:0016021 P9WFJ5 NC_000962.3 Mycobrowser_v5 CDS 2632923 2634098 . - 0 Rv2352c PPE38 Function unknown PPE family protein PPE38 Rv2352c, (MTCY98.21c), len: 391 aa. PPE38, Member of Mycobacterium tuberculosis PPE_family, highly similar to many e.g. Q10778|MTCY48.17|Y04H_MYCTU (734 aa), FASTA scores: opt: 713, E(): 2.8e-27, (37.7% identity in 430 aa overlap); Q10540|MTCY31.06c, Q11031|MTCY02B10.25c, Q10813|MTCY274.23c, P42611|MTV037.06C, P71868|MTCY03C7.23, P95248|MTCY98.22c, P71869|MTCY03C7.24c, etc. P9WHZ9 No PE/PPE Q7D7A2 P9WHZ9 P9WHZ9 NC_000962.3 Mycobrowser_v5 CDS 2637688 2639535 . - 0 Rv2356c PPE40 Function unknown PPE family protein PPE40 Rv2356c, (MTCY98.25), len: 615 aa. PPE40, Member of Mycobacterium tuberculosis PPE_family, highly similar to others e.g. Q10778|MTCY48.17|YF48_MYCTU hypothetical PPE-family protein (678 aa), FASTA scores: opt: 1888, E(): 1.9e-78, (54.4% identity in 667 aa overlap); Q10540|MTCY31.06c, E241779|MTCY98, P42611|MTV037.06c, Q10813|MTCY274.23c, P71657|MTCY02B10.25c, MTCY03C7.23, P71869|MTCY03C7.24c, etc. Predicted to be an outer membrane protein (See Song et al., 2008). P9WHZ7 No PE/PPE Q7D7A1 P9WHZ7 P9WHZ7 NC_000962.3 Mycobrowser_v5 CDS 2692224 2692439 . + 0 Rv2395A aprA Unknown Acid and phagosome regulated protein A AprA Rv2395A, len: 71 aa. AprA, acid and phagosome regulated protein A, restricted to M. tuberculosis complex. Note completely overlapped by sRNA mcr7. V5QPR9 No conserved hypotheticals Q8VJK0 V5QPR9 V5QPR9 NC_000962.3 Mycobrowser_v5 CDS 2628781 2630319 . - 0 Rv2350c plcB Hydrolyzes sphingomyelin in addition to phosphatidylcholine. Probable virulence factor implicated in the pathogenesis of Mycobacterium tuberculosis at the level of intracellular survival, by the alteration of cell signaling events or by direct cytotoxicity [catalytic activity: a phosphatidylcholine + H(2)O = 1,2- diacylglycerol + choline phosphate]. Membrane-associated phospholipase C 2 PlcB Rv2350c, (MT2415, MTCY98.19c), len: 512 aa. plcB (alternate gene name: mpcB), membrane-associated phospolipase C 2 (see citations below), similar to other precursors of several phospolipases C e.g. P15713|PHLN_PSEAE|PA3319 non-hemolytic phospholipase C precursor from Pseudomonas aeruginosa (692 aa), FASTA scores: opt: 885, E(): 2.3e-44, (38.5% identity in 525 aa overlap); P06200|PHLC_PSEAE hemolytic phospholipase C precursor from Pseudomonas aeruginosa (730 aa), FASTA scores: opt: 639, E(): 6.3e-30, (537 aa overlap); Q9RGS8 non-hemolytic phospholipase C from Pseudomonas aeruginosa (700 aa), FASTA scores: opt: 864, E(): 3.9e-43, (39.2% identity in 528 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. Q50560|Rv2351c|PLCA|MTP40|MT2416|MTCY98.20c phospholipase C 1 (520 aa), FASTA scores: opt: 2788, E(): 4.5e-156, (75.5% identity in 514 aa overlap); Q9XB13|PLCD|Rv1755c|MT1799|MTCY28.21C phospholipase C 4 (514 aa), FASTA scores: opt: 2623, E(): 2.1e-146, (71.5% identity in 512 aa overlap); P95245|PLCC|Rv2349c|MT2414|MTCY98.18c phospholipase C 3 (508 aa), FASTA scores: opt: 2474, E(): 1.1e-137, (67.65% identity in 513 aa overlap); etc. Belongs to the bacterial phospholipase C family. Supposed membrane-associated, at the extracellular side. Substrate of Tat pathway (See McDonough et al., 2008). P9WIB3 No intermediary metabolism and respiration P95246 P9WIB3 GO:0034480,GO:0005886,GO:0009405 3.1.4.3 P9WIB3 NC_000962.3 Mycobrowser_v5 CDS 2630537 2632075 . - 0 Rv2351c plcA Hydrolyzes sphingomyelin in addition to phosphatidylcholine. Probable virulence factor implicated in the pathogenesis of Mycobacterium tuberculosis at the level of intracellular survival, by the alteration of cell signaling events or by direct cytotoxicity [catalytic activity: a phosphatidylcholine + H(2)O = 1,2- diacylglycerol + choline phosphate]. Membrane-associated phospholipase C 1 PlcA (MTP40 antigen) Rv2351c, (MTP40, MT2416, MTCY98.20c), len: 512 aa. plcA (alternate gene name: mpcA), membrane-associated phospolipase C 1 (MTP40 antigen) (see citations below), similar to other precursors of several phospolipases C e.g. P15713|PHLN_PSEAE|PA3319 non-hemolytic phospholipase C precursor from Pseudomonas aeruginosa (692 aa), FASTA scores: opt: 1064, E(): 4.3e-55, (39.85% identity in 517 aa overlap); P06200|PHLC_PSEAE hemolytic phospholipase C precursor from Pseudomonas aeruginosa (730 aa), FASTA scores: opt: 562, E(): 1.6e-25, (35.35% identity in 481 aa overlap); Q9RGS8|PLCN|PHLN_BURPS non-hemolytic phospholipase C from Burkholderia pseudomallei (Pseudomonas pseudomallei) (700 aa), FASTA scores: opt: 843, E(): 4.4e-42, (40.5% identity in 531 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. P95246|PLCB|MPCB|Rv2350c|MT2415|MTCY98.19c phospholipase C 2 (512 aa), FASTA scores: opt: 2788, E(): 1.2e-156, (75.5% identity in 514 aa overlap) (alias Q50561|PLCB|MPCB|Rv2350c|MT2415|MTCY98.19c phospholipase C 2 (521 aa), FASTA scores: opt: 2700, E(): 1.8e-151, (73.8% identity in 515 aa overlap)); Q9XB13|PLCD|Rv1755c|MT1799|MTCY28.21C phospholipase C 4 (514 aa), FASTA scores: opt: 2643, E(): 4.1e-148, (71.6% identity in 511 aa overlap); etc. Belongs to the bacterial phospholipase C family. Supposed membrane-associated, at the extracellular side. P9WIB5 No intermediary metabolism and respiration Q04001 P9WIB5 GO:0034480,GO:0005886,GO:0009405 3.1.4.3 P9WIB5 NC_000962.3 Mycobrowser_v5 CDS 2641650 2642042 . + 0 Rv2359 zur Acts as a global negative controlling element, employing Zn(2+) as a cofactor to bind the operator of the repressed genes. Probable zinc uptake regulation protein Zur Rv2359, (MTCY27.21c), len: 130 aa. Probable zur, zinc uptake regulation protein, equivalent to FURB|ML0824|Q9CCG6 putative ferric uptake regulatory protein from Mycobacterium leprae (131 aa), FASTA scores: opt: 765, E(): 1.7e-43, (86.9% identity in 130 aa overlap). Also highly similar to ferric uptake regulation proteins e.g. Q9L2H5|SCC121.11 putative metal uptake regulation protein from Streptomyces coelicolor (139 aa), FASTA scores: opt: 547, E(): 3.4e-29, (59.4% identity in 133 aa overlap); P06975|FUR_ECOLI from Escherichia coli (148 aa), FASTA scores: opt: 322, E(): 1.9e-14, (37.9% identity in 132 aa overlap); P45599|FUR_KLEPN ferric uptake regulation protein from Klebsiella pneumoniae (155 aa), FASTA scores: opt: 314, E(): 6.7e-14, (36.35% identity in 132 aa overlap); etc. Belongs to the fur/ZUR family. Note that previously known as furB. P9WN85 No regulatory proteins 2O03 O05839 P9WN85 GO:0003700,GO:0006355 P9WN85 NC_000962.3 Mycobrowser_v5 CDS 2692551 2692715 . + 0 Rv2395B aprB Unknown Acid and phagosome regulated protein B AprB Rv2395B, len: 54 aa. AprB, acid and phagosome regulated protein B, restricted to M. tuberculosis complex. V5QRX2 No conserved hypotheticals Q8VJJ9 V5QRX2 NC_000962.3 Mycobrowser_v5 CDS 2641246 2641653 . + 0 Rv2358 smtB Involved in transcriptional mechanism. Probable transcriptional regulatory protein SmtB (probably ArsR-family) Rv2358, (MTCY27.22c), len: 135 aa. Probable smtB, transcriptional regulator, arsR family, equivalent to Q9CCG5|ML0825 putative ArsR-family transcriptional regulator from Mycobacterium leprae (140 aa), FASTA scores: opt: 647, E(): 2e-34, (72.9% identity in 140 aa overlap). Also similar to others e.g. BAB48273|MLR0745 Transcriptional regulator from Rhizobium loti (Mesorhizobium loti) (104 aa), FASTA scores: opt: 185, E(): 3.4e-05, (43.25% identity in 74 aa overlap) (has its N-terminus shorter); P15905|ARR1_ECOLI arsenical resistance operon repressor from Escherichia coli (117 aa), FASTA scores: opt: 164, E(): 8.1e-05, (39.1% identity in 69 aa overlap); etc. Also similar to O53838|Rv0827|MTV043.19c putative transcriptional regulator from Mycobacterium tuberculosis (130 aa), FASTA scores: opt: 201, E(): 4e-06, (35.7% identity in 98 aa overlap); and O69711|Rv3744|MTV025.092 putative regulatory protein from Mycobacterium tuberculosis (120 aa), FASTA scores: opt: 209, E(): 1.2e-06, (35.5 % identity in 93 aa overlap). Contains possible helix-turn-helix motif at aa 72-93 (Score 1103, +2.94 SD). Belongs to the ArsR family of transciptional regulators. Shown to bind palindromic DNA sequence upstream of Rv2358; inhibited by Zn2+ (See Canneva et al., 2005). P9WMI5 No regulatory proteins O05840 P9WMI5 GO:0006355,GO:0006350,GO:0003700,GO:0005622 P9WMI5 NC_000962.3 Mycobrowser_v5 CDS 2642578 2643468 . - 0 Rv2361c Rv2361c Involved in the synthesis of decaprenyl diphosphate, a molecule which has a central role in the biosynthesis of most features of the mycobacterial cell wall. Adds seven more isoprene UNITS to omega,E, Z-farnesyl diphosphate and releases decaprenyl diphosphate. Long (C50) chain Z-isoprenyl diphosphate synthase (Z-decaprenyl diphosphate synthase) Rv2361c, (MT2430, MTCY27.19), len: 296 aa. Long (C50) chain Z-isoprenyl diphosphate synthase (see citation below), equivalent to UPPS_MYCLE|ML0634|B1937_F2_65|P38119 undecaprenyl pyrophosphate synthetase from Mycobacterium leprae (296 aa), FASTA scores: opt: 1789, E(): 1.8e-97, (86.5% identity in 296 aa overlap). Also highly similar to others e.g. UPPS|Q9L2H4 undecaprenyl pyrophosphate synthetase from Streptomyces coelicolor (277 aa), FASTA scores: opt: 1098, E(): 8.2e-60, (63.5% identity in 247 aa overlap); Q55482|UPPS_SYNY3|SLL0506 from Synechocystis sp. strain PCC 6803 (249 aa), FASTA scores: opt: 686, E(): 4.2e-33, (46.4% identity in 235 aa overlap); O67291|UPPS_AQUAE|AQ_1248 from Aquifex aeolicus (231 aa), FASTA scores: opt: 684, E(): 5.2e-33, (46.3% identity in 229 aa overlap); etc. Also similar to Rv1086|MTV017.39 from Mycobacterium tuberculosis. Contains PS01066 Hypothetical YBR002c family signature. Seems to belong to the UPP synthetase family. Note that previously known as uppS. P9WFF7 No cell wall and cell processes 2VG2,2VG3,2VG4 P60479 P9WFF7 GO:0051301,GO:0007047,GO:0008834,GO:0009252,GO:0008360,GO:0007049 2.5.1.31 P9WFF7 NC_000962.3 Mycobrowser_v5 CDS 2692799 2693884 . + 0 Rv2396 PE_PGRS41 Function unknown PE-PGRS family protein PE_PGRS41 Rv2396, (MTCY253.25c), len: 361 aa. PE_PGRS41, member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below). Also known as aprC, acid and phagosome regulated protein C, restricted to M. tuberculosis complex (See Abramovitch et al., 2011). Contains PS00583 pfkB family of carbohydrate kinases signature 1. Predicted to be an outer membrane protein (See Song et al., 2008). Q79FE6 No PE/PPE Q79FE6 Q79FE6 Q79FE6 NC_000962.3 Mycobrowser_v5 CDS 2645771 2646673 . - 0 Rv2364c era Binds both GDP and GTP. Has an intrinsic GTPase activity and is essential for cell growth. Probable GTP-binding protein Era Rv2364c, (MT2433, MTCY27.16), len: 300 aa. Probable era, GTP-binding protein, equivalent to Q49768|ERA_MYCLE|ML0631|B1937_F3_102 GTP-binding protein era homolog from Mycobacterium leprae (300 aa) FASTA scores: opt: 1589, E(): 3.4e-88, (81.4% identity in 301 aa overlap). Also highly similar to other GTP-binding proteins e.g. Q9RDF2|ERA_STRCO|SCC77.06 from Streptomyces coelicolor (317 aa), FASTA scores: opt: 1264, E(): 1.1e-68, (64.0% identity in 306 aa overlap); Q9KD52|ERA_BACHD|BH1367|BEX from Bacillus halodurans (304 aa), FASTA scores: opt: 869, (44.8% identity in 297 aa overlap); Q9KIH7|ERA_LACLA|ERAL from Lactococcus lactis (subsp. lactis) (Streptococcus lactis), and Lactococcus lactis (subsp. cremoris) (Streptococcus cremoris) (303 aa), FASTA scores: opt: 781, E(): 9.4e-40, (40.25% identity in 298 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the era/TRME family of GTP-binding proteins, era subfamily. Note that previously known as bex. P9WNK9 No intermediary metabolism and respiration P0A562 P9WNK9 GO:0003723,GO:0005622,GO:0005525 P9WNK9 NC_000962.3 Mycobrowser_v5 CDS 2647060 2648367 . - 0 Rv2366c Rv2366c Function unknown Probable conserved transmembrane protein Rv2366c, (MTCY27.14), len: 435 aa. Probable conserved transmembrane protein, highly similar to Q9L2L3|SCC117.07 putative membrane protein from Streptomyces coelicolor (358 aa), FASTA scores: opt: 1159, E(): 5.5e-64, (53.0% identity in 353 aa overlap); ans similar to hypothetical proteins and hemolysin-related proteins e.g. Q9HN02|HLP|VNG2308G hemolysin protein from Halobacterium sp. strain NRC-1 (457 aa), FASTA scores: opt: 623, E(): 6.2e-31, (28.4% identity in 433 aa overlap); etc. Potential transmembrane protein with 2 CBS domains. Belongs to the UPF0053 family. P9WFP1 No cell wall and cell processes P67130 P9WFP1 GO:0005886,GO:0016021 P9WFP1 NC_000962.3 Mycobrowser_v5 CDS 2648364 2648912 . - 0 Rv2367c Rv2367c Function unknown Conserved hypothetical protein Rv2367c, (MTCY27.13), len: 182 aa. Conserved hypothetical protein, equivalent to Q49752|YN67_MYCLE|ML0628|B1937_F1_21 hypothetical 19.8 KDA protein from Mycobacterium leprae (178 aa), FASTA scores: opt: 1051, E(): 2e-59, (89.1% identity in 175 aa overlap). Also highly similar to others e.g. Q9L2L4|SCC117.06 conserved hypothetical protein from Streptomyces coelicolor (165 aa), FASTA scores: opt: 599, E(): 6e-31, (56.5% identity in 154 aa overlap); Q9KD56|BH1363 hypothetical protein from Bacillus halodurans (159 aa), FASTA scores: opt: 311, E(): 8.3e-13, (45.05% identity in 111 aa overlap); etc. P9WGX9 No conserved hypotheticals P67134 P9WGX9 GO:0008270,GO:0008237 3.4.24.- P9WGX9 NC_000962.3 Mycobrowser_v5 CDS 2675936 2677633 . + 0 Rv2384 mbtA Involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins (initiation step of mycobactin chain growth). Activates the mycobactin ArCP in two half-reactions: activates salicylic acid as acyladenylate (adenylation step) + transfers activated salicylate to the MBTA ArCP as a thioester (arylation step). Bifunctional enzyme MbtA: salicyl-AMP ligase (SAL-AMP ligase) + salicyl-S-ArCP synthetase Rv2384, (MTCY22H8.01, MTCY253.37c), len: 565 aa. mbtA, bifunctional enzyme, including salicyl-AMP ligase (Sal-AMP ligase) and salicyl-S-ArCP synthetase (see Quadri et al., 1998; De Voss et al., 1999), highly similar to other ligases e.g. Q9F638|MXCE from Stigmatella aurantiaca 2,3-DHBA-AMP ligase (protein involved in the biosynthesis of 2,3-dihydroxybenzoic acid, contains the AMP binding signature) (543 aa), FASTA scores: opt: 1683, E(): 2.8e-90, (48.25% identity in 545 aa overlap) (see Silakowski et al., 2000); P40871|DHBE_BACSU|ENTE 2,3-dihydroxybenzoate-AMP ligase from Bacillus subtilis (539 aa), FASTA scores: opt: 1569, E(): 1.2e-83, (44.9% identity in 532 aa overlap); O07899|VIBE_VIBCHVC0772 vibriobactin-specific 2,3-dihydroxybenzoate-AMP ligase from Vibrio cholerae (543 aa), FASTA scores: opt: 1457, E(): 3.7e-77, (44.6% identity in 545 aa overlap); etc. Also similar to P95819|SNBA pristinamycin I synthetase I from Streptomyces pristinaespiralis (582 aa), FASTA scores: opt: 1532, E(): 1.7e-81, (46.35% identity in 548 aa overlap); and Q9RFM9|PCHD salicyl-AMP ligase from Pseudomonas aeruginosa (547 aa), FASTA scores: opt: 1415, E(): 1e-74, (45.95% identity in 533 aa overlap). Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family. P71716 No lipid metabolism P71716 P71716 GO:0008152,GO:0016874 6.-.-.- P71716 NC_000962.3 Mycobrowser_v5 CDS 2651753 2651938 . + 0 Rv2371 PE_PGRS40 Function unknown PE-PGRS family protein PE_PGRS40 Rv2371, (MTCY27.09c), len: 61 aa. PE_PGRS40, Short protein, member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to N-terminal part of others e.g. AAK44356|MT0132 PE_PGRS family protein from Mycobacterium tuberculosis strain CDC1551 (561 aa), FASTA scores: opt: 217, E(): 4.9e-08, (69.65% identity in 56 aa overlap); etc. Q79FE9 No PE/PPE Q79FE9 Q79FE9 Q79FE9 NC_000962.3 Mycobrowser_v5 CDS 2649946 2650248 . - 0 Rv2369c Rv2369c Unknown Hypothetical protein Rv2369c, (MTCY27.11), len: 100 aa. Hypothetical unknown protein. L0TC46 No conserved hypotheticals L0TC46 L0TC46 NC_000962.3 Mycobrowser_v5 CDS 2650245 2651558 . - 0 Rv2370c Rv2370c Function unknown Conserved hypothetical protein Rv2370c, (MTCY27.10), len: 437 aa. Conserved hypothetical protein, member of family proteins from Mycobacterium tuberculosis with Rv1453|MTCY493_01c|O06807 conserved hypothetical protein from Mycobacterium tuberculosis (432 aa), FASTA scores: opt: 1943, E(): 9.4e-115, (69.9% identity in 409 aa overlap); Rv1194c|MTCI364.06c; etc. Also similar to AAK45764|MT1500 conserved hypothetical protein from Mycobacterium tuberculosis strain CDC1551 (432 aa), FASTA scores: opt: 1934, E(): 9.4e-115, (69.9% identity in 409 aa overlap). O05828 No conserved hypotheticals O05828 O05828 NC_000962.3 Mycobrowser_v5 CDS 3121501 3121827 . - 0 Rv2815c Rv2815c Required for the transposition of the insertion element IS6110. Probable transposase Rv2815c, (MTCY16B7.28), len: 108 aa. Putative Transposase for IS6110 (fragment). Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2814c and Rv2815c, the sequence UUUUAAAG (directly upstream of Rv2814c) maybe responsible for such a frameshifting event (see McAdam et al., 1990). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WKH5 No insertion seqs and phages P0C5G9 P9WKH5 GO:0004803,GO:0006313,GO:0003677 P9WKH5 NC_000962.3 Mycobrowser_v5 CDS 2652839 2653987 . - 0 Rv2373c dnaJ2 Acts as a co-chaperone. Stimulates, jointly with GRPE, the ATPase activity of DNAK|Rv0350. Probable chaperone protein DnaJ2 Rv2373c, (MTCY27.07), len: 382 aa. Probable dnaJ2, chaperone protein, equivalent to Q49762|DNJ2_MYCLE|ML0625|B1937_F2_56 chaperone protein from Mycobacterium leprae (378 aa), FASTA scores: opt: 2301, E(): 1.7e-120, (87.5% identity in 382 aa overlap). Also highly similar to other chaperone proteins DNAJ/DNAJ2 e.g. Q9RDD7|DNJ2_STRCO|SCC77.21c from Streptomyces coelicolor (378 aa), FASTA scores: opt: 1456, E(): 1.2e-73, (54.8% identity in 385 aa overlap); O52164|DNJ2_STRAL from Streptomyces albus (379 aa) FASTA scores: opt: 1378, E(): 2.6e-69, (52.2% identity in 385 aa overlap); Q9S5A3|DNAJ_LISMO from Listeria monocytogenes (377 aa), FASTA scores: opt: 1013, E(): 4.6e-49, (41.3% identity in 385 aa overlap); etc. Also similar to Rv0352|MTCY13E10.12 from Mycobacterium tuberculosis. Contains 1 J domain and 1 cr domain. Belongs to the DNAJ family. P9WNV7 No virulence, detoxification, adaptation P63966 P9WNV7 GO:0006260,GO:0005737,GO:0031072,GO:0046872,GO:0006457,GO:0009408,GO:0051082,GO:0005524 P9WNV7 NC_000962.3 Mycobrowser_v5 CDS 2703269 2703838 . + 0 Rv2405 Rv2405 Function unknown Conserved protein Rv2405, (MTCY253.15c), len: 189 aa. Conserved protein, identical (but N-terminus longer 40 residues) to AAK46773|MT2477 hypothetical protein from Mycobacterium tuberculosis strain CDC1551. Also highly similar, but N-terminus longer 38 residues, to Q9RD03|SCCM1.41 hypothetical 17.4 KDA protein from Streptomyces coelicolor (154 aa), FASTA scores: opt: 451, E(): 2e-22, (48.7% identity in 154 aa overlap). Shows also similarity with hypothetical proteins from other species. P71738 No conserved hypotheticals P71738 P71738 GO:0003677 P71738 NC_000962.3 Mycobrowser_v5 CDS 2655609 2656115 . - 0 Rv2376c cfp2 Function not known (putative secreted protein); may play a role in the development of protective immune responses. Low molecular weight antigen CFP2 (low molecular weight protein antigen 2) (CFP-2) Rv2376c, (MT2445, MTCY27.04), len: 168 aa. Cfp2 (alternate gene name: mtb12), low molecular weight antigen, secreted protein similar to Q49771|MB12_MYCLE|ML0620|B1937_F3_91 low molecular weight antigen MTB12 homolog precursor from Mycobacterium leprae (167 aa), FASTA scores: opt: 682, E(): 1.7e-32, (65.5% identity in 165 aa overlap). Belongs to the MTB12 family. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). P9WIN7 No cell wall and cell processes P9WIN7 GO:0005576 NC_000962.3 Mycobrowser_v5 CDS 2655265 2655582 . + 0 Rv2375 Rv2375 Function unknown Conserved hypothetical protein Rv2375, (MTCY27.05c), len: 105 aa. Conserved hypothetical protein, highly similar to only CAC32314|2SCD60.09c conserved hypothetical protein from Streptomyces coelicolor (98 aa), FASTA scores: opt: 425, E(): 5.7e-24, (63.25% identity in 98 aa overlap). O05823 No conserved hypotheticals O05823 O05823 NC_000962.3 Mycobrowser_v5 CDS 2739772 2740032 . - 0 Rv2441c rpmA Involved in translation mechanisms. 50S ribosomal protein L27 RpmA Rv2441c, (MTCY428.05), len: 86 aa. rpmA, 50S ribosomal proteins L27, equivalent to Q9CBZ3|RL27_MYCLE from Mycobacterium leprae (88 aa), FASTA scores: opt: 504, E(): 7.6e-28, (93.2% identity in 81 aa overlap). Also highly similar to others e.g. P95757|RL27_STRGR from Streptomyces griseus (85 aa), FASTA scores: opt: 442, E(): 1.2e-23, (81.5% identity in 81 aa overlap); etc. Contains PS00831 Ribosomal protein L27 signature. Belongs to the L27P family of ribosomal proteins. P9WHB3 No information pathways P66127 P9WHB3 GO:0003735,GO:0006412,GO:0005840 P9WHB3 NC_000962.3 Mycobrowser_v5 CDS 2662067 2667115 . - 0 Rv2380c mbtE Involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins. Probably activates the two lysine residues that are incorporated into mycobactin (lysine ligation). Peptide synthetase MbtE (peptide synthase) Rv2380c, (MTCY22H8.05), len: 1682 aa. MbtE, peptide synthetase (see citations below), similar in part to several synthases e.g. O07944|SNBDE pristinamycin I synthase 3 and 4 from Streptomyces pristinaespiralis (4848 aa), FASTA scores: opt: 2635, E(): 1.9e-146, (36.8% identity in 1657 aa overlap); O05647|SNBDE virginiamycin S synthetase (fragment) from Streptomyces virginiae (1997 aa) FASTA scores: opt: 2580, E(): 1.6e-143, (40.65% identity in 1163 aa overlap); Q9R9I2|DHBF protein involved in siderophore production from Bacillus subtilis (2378 aa), FASTA scores: opt: 2388, E(): 3.6e-132, (33.9% identity in 1579 aa overlap); O68487|ACMB actinomycin synthetase II from Streptomyces chrysomallus (2611 aa), FASTA scores: opt: 2165, E(): 4.9e-119, (35.0% identity in 1634 aa overlap); etc. Equivalent to AAK46743 from Mycobacterium tuberculosis strain CDC1551 (1787 aa) but shorter 105 aa. Contains PS00455 putative AMP-binding domain signature, and PS00012 Phosphopantetheine attachment site. Belongs to the ATP-dependent AMP-binding enzyme family. I6Y0L1 No lipid metabolism O86329 I6Y0L1 GO:0048037,GO:0016874,GO:0008152,GO:0031177,GO:0000036 I6Y0L1 NC_000962.3 Mycobrowser_v5 CDS 2671593 2675837 . - 0 Rv2383c mbtB Involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins. This peptide synthase forms amide bound between the carboxylic acid of salicylate and the alpha-amino group of serine (serine/threonine ligation). Phenyloxazoline synthase MbtB (phenyloxazoline synthetase) Rv2383c, (MTCY22H8.02), len: 1414 aa. MbtB, phenyloxazoline synthase (see citations below), similar to the N-terminal region of several synthetases e.g. Q9EWP5|SC4C2.17 putative non-ribosomal peptide synthase from Streptomyces coelicolor (2229 aa), FASTA scores: opt: 2878, E(): 4.1e-156, (46.85% identity in 1138 aa overlap); Q9Z399|IRP2 yersiniabactin biosynthetic from Yersinia pestis (2041 aa), FASTA scores: opt: 2297, E(): 5.3e-123, (38.55% identity in 1069 aa overlap); P48633|HMP2_YEREN|IRP2 high-molecular-weight protein 2 (may be involved in the nonribosomal synthesis of small peptides) from Yersinia enterocolitica (2035 aa), FASTA scores: opt: 2275, E(): 9.4e-122, (38.45% identity in 1069 aa overlap); O85739|PCHE|PA4226 dihydroaeruginoic acid synthetase from Pseudomonas aeruginosa (1438 aa) FASTA scores: opt: 2236, E(): 1.2e-119, (38.2% identity in 1330 aa overlap); Q9RFM8|PCHE pyochelin synthetase from Pseudomonas aeruginosa (1438 aa), FASTA scores: opt: 2229, E(): 3e-119, (38.0% identity in 1329 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature, and PS00012 Phosphopantetheine attachment site. Belongs to the ATP-dependent AMP-binding enzyme family. P9WQ63 No lipid metabolism P71717 P9WQ63 GO:0009058,GO:0048037,GO:0016788,GO:0006826,GO:0016874,GO:0031177,GO:0000036 6.3.2.- P9WQ63 NC_000962.3 Mycobrowser_v5 CDS 2657700 2662085 . - 0 Rv2379c mbtF Involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins. Probably activates the two lysine residues that are incorporated into mycobactin (lysine ligation). Peptide synthetase MbtF (peptide synthase) Rv2379c, (MTCY27.01), len: 1461 aa. MbtF, peptide synthetase (see citations below), similar in part to several synthases e.g. O52820|PCZA363.4 protein from Amycolatopsis orientalis (4077 aa), FASTA scores: opt: 1873, E(): 1.1e-99, (35.55% identity in 1522 aa overlap); O07944|SNBDE pristinamycin I synthase 3 and 4 from Streptomyces pristinaespiralis (4848 aa), FASTA scores: opt: 1817, E(): 2.1e-96, (33.65% identity in 1463 aa overlap); O52821 protein similar to peptide synthetase from Amycolatopsis orientalis (1860 aa) FASTA scores: opt: 1705, E(): 2.9e-90, (34.75% identity in 1344 aa overlap); Q9XCF2|PSTB putative peptide synthetase (similar to Mycobacterium tuberculosis nrp protein) from Mycobacterium avium (2552 aa), FASTA scores: opt: 1687, E(): 4e-89, (35.45% identity in 1058 aa overlap); Q9ZET7 peptide synthetase (fragment) from Mycobacterium smegmatis (1438 aa), FASTA scores: opt: 1479, E(): 2.5e-77, (30.45% identity in 1507 aa overlap); etc. Contains PS00455 putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family. O05819 No lipid metabolism O05819 O05819 GO:0048037,GO:0016874,GO:0008152,GO:0000036 O05819 NC_000962.3 Mycobrowser_v5 CDS 2670269 2671603 . - 0 Rv2382c mbtC Involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins. Polyketide synthetase MbtC (polyketide synthase) Rv2382c, (MTCY22H8.03), len: 444 aa. MbtC, polyketide synthase (see citations below), similar in part to several synthases e.g. Q9F7T9 avermectin polyketide synthase (fragment) from Streptomyces avermitilis (3626 aa), FASTA scores: opt: 1458, E(): 7e-82, (50.65% identity in 446 aa overlap); AAG23264|SPNA polyketide synthase loading and extender module 1 from Saccharopolyspora spinosa (2595 aa) FASTA scores: opt: 1441, E(): 6e-81, (49.1% identity in 446 aa overlap); O33954|TYLG tylactone synthase starter module and modules 1 & 2 from Streptomyces fradiae (4472 aa) FASTA scores: opt: 1439, E(): 1.2e-80, (51.0% identity in 447 aa overlap); O30764|NIDA1 polyketide synthase modules 1 and 2 from Streptomyces caelestis (4340 aa) FASTA scores: opt: 1432, E(): 3.3e-80, (50.9% identity in 442 aa overlap); etc. P71718 No lipid metabolism P71718 P71718 GO:0016740,GO:0009058 P71718 NC_000962.3 Mycobrowser_v5 CDS 2697728 2698057 . + 0 Rv2401 Rv2401 Unknown Hypothetical protein Rv2401, (MTCY253.19c), len: 109 aa. Hypothetical unknown protein. Equivalent to AAK46768 from Mycobacterium tuberculosis strain CDC1551 (134 aa) but shorter 25 aa. N-terminus extended since first submission (previously 72 aa). O86326 No conserved hypotheticals O86326 NC_000962.3 Mycobrowser_v5 CDS 2727967 2728266 . - 0 Rv2431c PE25 Function unknown PE family protein PE25 Rv2431c, (MTCY428.15), len: 99 aa. PE25, Member of the Mycobacterium tuberculosis PE family (see Brennan & Delogu 2002), similar to others e.g. AAK47158|MT2839 from Mycobacterium tuberculosis (275 aa) FASTA scores: opt: 194, E(): 2.5e-06, (40.0% identity in 95 aa overlap); etc. I6X486 No PE/PPE 2G38 Q7D756 I6X486 I6X486 NC_000962.3 Mycobrowser_v5 CDS 2680765 2682018 . + 0 Rv2387 Rv2387 Function unknown Conserved protein Rv2387, (MTCY253.34c), len: 417 aa. Conserved protein, showing some similarities with others e.g. Q9K663|BH3869 hypothetical protein from Bacillus halodurans (337 aa), FASTA scores: opt: 343, E(): 4.8e-14, (29.0% identity in 400 aa overlap); AAK25471|CC3509 hypothetical protein from Caulobacter crescentus (365 aa), FASTA scores: opt: 282, E(): 3.2e-10, (32.6% identity in 399 aa overlap); P73953|SLR1512 [D90911_21] conserved hypothetical protein from Synechocystis sp. strain PCC6803 (374 aa), FASTA scores: opt: 230, E(): 5.5e-07; (24.75% identity in 408 aa overlap); etc. Contains PS00213 Lipocalin signature. P71757 No conserved hypotheticals P71757 P71757 NC_000962.3 Mycobrowser_v5 CDS 2682015 2683142 . - 0 Rv2388c hemN Involved in porphyrin biosynthesis. Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX. Probable oxygen-independent coproporphyrinogen III oxidase HemN (coproporphyrinogenase) (coprogen oxidase) Rv2388c, (MTCY253.33), len: 375 aa. Probable hemN, oxygen-independent coproporphyrinogen III oxidases, highly similar to many putative oxygen-independent coproporphyrinogen III oxidases e.g. Q9RDD2|SCC77.26 from Streptomyces coelicolor (435 aa), FASTA scores: opt: 1358, E(): 1.5e-76, (56.55% identity in 382 aa overlap); BAB51237|MLR4627 from Rhizobium loti (Mesorhizobium loti) (392 aa), FASTA scores: opt: 696, E(): 1.1e-35, (36.8% identity in 383 aa overlap); Q9KUR0|VC0455 from Vibrio cholerae (391 aa), FASTA scores: opt: 691, 2.2e-35, (32.65% identity in 386 aa overlap); P54304|HEMN_BACSU from Bacillus subtilis (366 aa), FASTA scores: opt: 668 , E(): 5.6e-34; (34.9% identity in 327 aa overlap); etc. Equivalent to AAK46752 from Mycobacterium tuberculosis strain CDC1551 (390 aa) but shorter 375 aa. Belongs to the anaerobic coproporphyrinogen III oxidase family. P9WP73 No intermediary metabolism and respiration P71756 P9WP73 GO:0051989,GO:0004109,GO:0005737,GO:0046872,GO:0055114,GO:0006779,GO:0051539 1.3.99.22 P9WP73 NC_000962.3 Mycobrowser_v5 CDS 2683248 2683712 . - 0 Rv2389c rpfD Promotes the resuscitation and growth of dormant, nongrowing cell. Could also stimulates the growth of several other high G+C gram+ organisms, e.g. Mycobacterium avium, Mycobacterium bovis (BCG), Mycobacterium kansasii, Mycobacterium smegmatis. Probable resuscitation-promoting factor RpfD Rv2389c, (MTCY253.32), len: 154 aa. Probable rpfD, resuscitation-promoting factor. Possible autocrine and/or paracrine bacterial growth factor or cytokine (see citation below). Similar to others from Mycobacterium tuberculosis e.g. O07747|Rv1884c|MTCY180.34|RPFC probable resuscitation-promoting factor from Mycobacterium tuberculosis (176 aa), FASTA scores: opt: 382, E(): 2.3e-17, (55.45% identity in 101 aa overlap); etc. Also similarity with Q9CBF8|ML2030 hypothetical protein from Mycobacterium leprae (157 aa), FASTA scores: opt: 397, E(): 2.4e-18, (47.95% identity in 121 aa overlap); Q9F2Q2|SCE41.06c putative secreted protein from Streptomyces coelicolor (244 aa), FASTA scores: opt: 341, E(): 1.1e-14, (40.45% identity in 131 aa overlap); and O86308|Z96935|MLRPF_1 RPF protein precursor from Micrococcus luteus (220 aa), FASTA scores: opt: 301, E(): 3.6e-12, (39.4% identity in 132 aa overlap). Contains a secretory signal sequence in N-terminus. Supposed acts at very low concentration. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WG27 No cell wall and cell processes P71755 P9WG27 GO:0005576 P9WG27 NC_000962.3 Mycobrowser_v5 CDS 2684679 2686370 . + 0 Rv2391 sirA Catalyzes the reduction of sulfite to sulfide, in the biosynthesis of sulfur-containing amino acids and co-factors Ferredoxin-dependent sulfite reductase SirA Rv2391, (MTCY253.30c), len: 563 aa. SirA, ferredoxin-dependent sulfite reductase (See Schnell et al., 2005). Previously annotated as nirA. Similar to e.g. CAC33947|SCBAC1A6.26c Putative nitrite/sulphite reductase from Streptomyces coelicolor (565 aa), FASTA scores: opt: 2335, E(): 1.2e-137, (60.1% identity in 567 aa overlap); Q9RZD6|DRA0013 ferredoxin-nitrite reductase from Deinococcus radiodurans (563 aa), FASTA scores: opt: 1141, E(): 2.2e-63, (39.6% identity in 533 aa overlap); Q59656|NIRA (D31732|PEENIRNRT_1) ferredoxin-dependent nitrite reductase from Plectonema boryanum (654 aa) (see Suzuki & Kikuchi 1995), FASTA scores: opt: 805, E(): 1.9e-42, (31.7% identity in 517 aa overlap); Q55366|NIRA|SLR0898 ferredoxin-nitrite reductase from Synechocystis sp. strain PCC 6803 (502 aa), FASTA scores: opt: 799, E(): 3.7e-42, (32.3% identity in 517 aa overlap); etc. Highly similar (only in N-terminal part because shortened protein (fragment) owing to an IS900 insertion) to Q9K541|NIRA nitrate reductase (fragment) from Mycobacterium paratuberculosis (198 aa), FASTA scores: opt: 798, E(): 2.1e-42, (65.4% identity in 182 aa overlap) (see Bull et al., 2000). P9WJ03 No intermediary metabolism and respiration 1ZJ8,1ZJ9 P71753 P9WJ03 GO:0020037,GO:0055114,GO:0050311,GO:0051539 1.8.7.1 P9WJ03 NC_000962.3 Mycobrowser_v5 CDS 2686367 2687131 . + 0 Rv2392 cysH Involved in the sulfate activation pathway (at the third step) in the reductive branch of the cysteine biosynthetic pathway. Reduces activated sulfate into sulfite [catalytic activity: 5-phosphoadenosine 3-phosphosulfate + reduced thioredoxin = phosphoadenosine phosphate + oxidized thioredoxin + sulfite]. Probable 3'-phosphoadenosine 5'-phosphosulfate reductase CysH (PAPS reductase, thioredoxin DEP.) (padops reductase) (3'- phosphoadenylylsulfate reductase) (PAPS sulfotransferase) Rv2392, (MTCY253.29c), len: 254 aa. Probable cysH, 3'-phosphoadenosine 5'-phosphosulfate reductase (see citation below), similar to many e.g. P94498|O34620|CYH1_BACSU|CYSH from Bacillus subtilis (233 aa), FASTA scores: opt: 618, E(): 8.1e-32, (46.5% identity in 202 aa overlap); Q9KCT3|CYSH|BH1486 from Bacillus halodurans (231 aa), FASTA scores: opt: 560, E(): 3.6e-28, (41.3% identity in 230 aa overlap); P56860|CYSH_DEIRA from Deinococcus radiodurans (255 aa), FASTA scores: opt: 489, E(): 1.1e-23, (44.7% identity in 190 aa overlap); etc. Belongs to the PAPS reductase family and CYSH subfamily. Note that operon cysA-cysW-cysT-subI, probably involved in sulfate transport, is near this putative ORF. P9WIK3 No intermediary metabolism and respiration P65668 P9WIK3 GO:0005737,GO:0055114,GO:0004604,GO:0019379,GO:0016740,GO:0019344 1.8.4.8 P9WIK3 NC_000962.3 Mycobrowser_v5 CDS 2723308 2724183 . - 0 Rv2426c Rv2426c Function unknown Conserved hypothetical protein Rv2426c, (MTCY428.21), len: 291 aa. Conserved hypothetical protein, highly similar to others e.g. Q51326|ORF4 from Pseudomonas carboxydovorans (295 aa), FASTA scores: opt: 853, E(): 3.7e-43, (48.75% identity in 277 aa overlap); BAB47746|MLR0088 from Rhizobium loti (309 aa), FASTA scores: opt :809, E(): 1.5e-40, (46.5% identity in 291 aa overlap); Q9Y9R8|APE2220 from Aeropyrum pernix (297 aa), FASTA scores: opt: 763, E(): 7.4e-38, (47.1% identity in 261 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). P71922 No conserved hypotheticals P71922 P71922 GO:0016887,GO:0005524 P71922 NC_000962.3 Mycobrowser_v5 CDS 2688010 2689941 . + 0 Rv2394 ggtB Plays a key role in the gamma-glutamyl cycle, a pathway for the synthesis and degradation of glutathione [catalytic activity: 5-L-glutamyl)-peptide + an amino acid = peptide + 5-L-glutamyl-amino acid]. Probable gamma-glutamyltranspeptidase precursor GgtB (gamma-glutamyltransferase) (glutamyl transpeptidase) Rv2394, (MTCY253.27c), len: 643 aa. Probable ggtB, gamma-glutamyltranspeptidase precursor, similar to many e.g. Q9KVF2|VC0194 from Vibrio cholerae (588 aa), FASTA scores: opt: 943, E(): 7.5e-47, (40.0% identity in 597 aa overlap); O69935|SC3C8.26 from Streptomyces coelicolor (603 aa), FASTA scores: opt: 822, E(): 7.2e-40, (33.6% identity in 622 aa overlap); P54422|GGT_BACSU from Bacillus subtilis (587 aa) FASTA scores: opt: 491, E(): 8.2e-21, (33.4% identity in 574 aa overlap); etc. Has potential signal peptide and appropriately positioned prokaryotic lipoprotein attachment site (PS00013). P71750 No intermediary metabolism and respiration P71750 P71750 GO:0003840,GO:0008415 2.3.2.2 P71750 NC_000962.3 Mycobrowser_v5 CDS 2704009 2704437 . - 0 Rv2406c Rv2406c Function unknown Conserved protein Rv2406c, (MTCY253.14), len: 142 aa. Conserved protein. C-terminal region is identical with many CBS domain protein e.g. AAK46774|MT2478 CBS domain protein from Mycobacterium tuberculosis strain CDC1551 (aa 47-142), FASTA scores: opt: 594, E(): 1.9e-30, (98.97% identity in 97 aa overlap); etc. Also similar to other hypothetical proteins e.g. AAK24594|CC2626 CBS domain protein from Caulobacter crescentus (157 aa), FASTA scores: opt: 377, E(): 8.3e-17, (42.55% identity in 141 aa overlap); BAB47826|MLR0188 from Rhizobium loti; etc. P71737 No conserved hypotheticals P71737 P71737 P71737 NC_000962.3 Mycobrowser_v5 CDS 2693909 2694964 . - 0 Rv2397c cysA1 Involved in the active transport across the membrane of multiple sulfur-containing compounds, including sulfate and thiosulfate (import). Responsible for energy coupling to the transport system. Sulfate-transport ATP-binding protein ABC transporter CysA1 Rv2397c, (MTCY253.24), len: 351 aa. cysA1, sulfate-transport ATP-binding protein ABC transporter (see citations below), similar to other sulfate ABC transporter ATP-binding proteins e.g. P14788|CYSA_SYNP7 from Synechococcus sp. (344 aa), FASTA scores: opt: 1112, E(): 2.6e-56, (54.6% identity in 328 aa overlap); P74548|CYSA_SYNY3 from Synechocystis sp. (355 aa), FASTA scores: opt: 1063, E(): 1.7e-53, (51.9% identity in 343 aa overlap); Q9I6L0|CYSA|PA0280 from Pseudomonas aeruginosa (329 aa), FASTA scores: opt: 987, E(): 3.3e-49, (49.2% identity in 339 aa overlap); etc. Also similar to many ATP-binding proteins from Mycobacterium tuberculosis e.g. Rv2038c, Rv1238, Rv2832c, etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). Note that previously known as cysA. P9WQM1 No cell wall and cell processes P0A4W2 P9WQM1 GO:0043190,GO:0005886,GO:0015419,GO:0008272,GO:0005524 3.6.3.25 P9WQM1 NC_000962.3 Mycobrowser_v5 CDS 2694981 2695799 . - 0 Rv2398c cysW Involved in the active transport across the membrane of multiple sulfur-containing compounds, including sulfate and thiosulfate (import). Responsible for the translocation of the substrate across the membrane. Probable sulfate-transport integral membrane protein ABC transporter CysW Rv2398c, (MTCY253.23), len: 272 aa. Probable cysW, sulfate-transport integral membrane protein ABC transporter (see citations below), similar to others e.g. Q9K877|CYSW|BH3129 sulfate ABC transporter (permease) from Bacillus halodurans (287 aa), FASTA scores: opt: 765, E(): 4.1e-40, (43.8% identity in 249 aa overlap); P27370|CYSW_SYNP7 sulfate transport system (permease) protein from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (286 aa), FASTA scores: opt: 757, E(): 1.3e-39, (44.3% identity in 264 aa overlap); Q9I6K9|CYSW|PA0281 sulfate transport protein from Pseudomonas aeruginosa (289 aa), FASTA scores: opt: 753, E(): 2.3e-39, (44.4% identity in 250 aa overlap); P16702|P76534|CYSW_ECOLI sulfate transport system permease from Escherichia coli (291 aa), FASTA scores: opt: 633, E(): 5.7e-32, (38.2% identity in 267 aa overlap); etc. Contains PS00402 Binding-protein-dependent transport systems inner membrane component signature. Similarity with integral membrane components of other binding-protein-dependent transport systems and belongs to the CYSTW subfamily. P71746 No cell wall and cell processes P71746 P71746 GO:0005886,GO:0015116,GO:0008272,GO:0015563,GO:0016021 P71746 NC_000962.3 Mycobrowser_v5 CDS 2695796 2696647 . - 0 Rv2399c cysT Involved in the active transport across the membrane of multiple sulfur-containing compounds, including sulfate and thiosulfate (import). Responsible for the translocation of the substrate across the membrane. Probable sulfate-transport integral membrane protein ABC transporter CysT Rv2399c, (MTCY253.22), len: 283 aa. Probable cysT, sulfate-transport integral membrane protein ABC transporter (see citations below), similar to others e.g. BAB48989|MLR1667 permease protein of sulfate ABC transporter from Rhizobium loti (283 aa), FASTA scores: opt: 756, E(): 7.9e-40, (40.95% identity in 271 aa overlap); Q9K878|cyst|BH3128 sulfate ABC transporter (permease) from Bacillus halodurans (279 aa), FASTA scores: opt: 750, E(): 1.8e-39, (44.55% identity in 258 aa overlap); P16701|CYST_ECOLI|CYSU|cyst|B2424 from Escherichia coli (277 aa), FASTA scores: opt: 669, E(): 1.9e-34, (40.0% identity in 260 aa overlap); etc. Contains PS00402 Binding-protein-dependent transport systems inner membrane component signature, and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the CYSTW subfamily. P71745 No cell wall and cell processes P71745 P71745 GO:0015116,GO:0008272,GO:0015563,GO:0016021 P71745 NC_000962.3 Mycobrowser_v5 CDS 2706017 2706736 . + 0 Rv2408 PE24 Function unknown Possible PE family-related protein PE24 Rv2408, (MTCY253.12c), len: 239 aa. Possibly PE24, a member of PE family (see citation below), similar to AAK46440|MT2159 from Mycobacterium tuberculosis strain CDC1551 (491 aa) FASTA scores: opt: 269, E(): 5.4e-08, (38.45% identity in 156 aa overlap) and AAK45466|MT1209 from Mycobacterium tuberculosis strain CDC1551 (308 aa), FASTA scores: opt: 265, E(): 6.3e-08, (36.0% identity in 197 aa overlap). Q79FE3 No PE/PPE Q79FE3 Q79FE3 Q79FE3 NC_000962.3 Mycobrowser_v5 CDS 2698529 2700457 . + 0 Rv2402 Rv2402 Function unknown Conserved protein Rv2402, (MTCY253.18c), len: 642 aa. Conserved protein, highly similar to others e.g. 9X8C4|SCE36.11c conserved hypothetical protein (fragment) from Streptomyces coelicolor (612 aa), FASTA scores: opt: 1283, E(): 6.5e-75, (41.9% identity in 623 aa overlap); Q9RJ38|SCI8.15 hypothetical 66.3 KDA protein from Streptomyces coelicolor (595 aa), FASTA scores: opt: 1152, E(): 1.7e-66, (39.9% identity in 622 aa overlap), Q9S223|CI51.17 hypothetical 68.4 KDA protein from Streptomyces coelicolor (612 aa), FASTA scores: opt: 1146, E(): 4.2e-66, (40.6% identity in 623 aa overlap); YAY3_SCHPO|Q10211|c4h3.03c hypothetical 74.5 kDa protein from Schizosaccharomyces pombe (Fission yeast) (649 aa) FASTA scores: opt: 999, E(): 1.3e-56, (35.0% identity in 642 aa overlap); etc. Contains possible helix-turn-helix motif, at aa 224-245 (+4.68 SD). P71741 No conserved hypotheticals P71741 P71741 GO:0005976,GO:0004339 P71741 NC_000962.3 Mycobrowser_v5 CDS 2700535 2701290 . - 0 Rv2403c lppR Unknown Probable conserved lipoprotein LppR Rv2403c, (MTCY253.17), len: 251 aa. Probable lppR, conserved lipoprotein, with weak similarity with mycobacterial serine/threonine protein kinases e.g. AAK45563|MT1304 from Mycobacterium tuberculosis strain CDC1551 (626 aa), FASTA scores: opt: 186, E(): 0.00023, (24.4% identity in 238 aa overlap), and the C-terminal part of Q11053|Rv1266c|MTCY50.16|PKNH_MYCTU from Mycobacterium tuberculosis (626 aa), FASTA scores: opt: 185, E()= 0.00027, (24.35% identity in 238 aa overlap). Has signal peptide and appropriate positioned prokaryotic lipoprotein attachment site (PS00013). Could belong to the Ser/Thr family of protein kinases. P71740 No cell wall and cell processes P71740 P71740 NC_000962.3 Mycobrowser_v5 CDS 2740047 2740361 . - 0 Rv2442c rplU Involved in translation mechanisms. 50S ribosomal protein L21 RplU Rv2442c, (MTCY428.04), len: 104 aa. rplU, 50S ribosomal protein L21, equivalent to Q9CBZ2|RL21_MYCLE from Mycobacterium leprae (103 aa), FASTA scores: opt: 579, E(): 4.8e-31, (91.1% identity in 102 aa overlap). Also highly similar to others e.g. P95756|RL21_STRGR from Streptomyces griseus (106 aa), FASTA scores: opt: 362, E(): 5.4e-17, (56.0% identity in 100 aa overlap); etc. P9WHC3 No information pathways P66117 P9WHC3 GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WHC3 NC_000962.3 Mycobrowser_v5 CDS 2704697 2705518 . + 0 Rv2407 Rv2407 Function unknown Conserved hypothetical protein Rv2407, (MTCY253.13c), len: 273 aa. Conserved hypothetical protein, highly similar (but longer at N-terminus) to AAK46775|MT2479 putative arylsulfatase from Mycobacterium tuberculosis strain CDC1551 (224 aa) FASTA scores: opt: 1433, E(): 2.5e-81, (96.43% identity in 224 aa overlap); O33130|MLCL536.01 hypothetical protein from Mycobacterium leprae (220 aa), FASTA scores: opt: 658, E(): 1.5e-33, (56.75% identity in 215 aa overlap). Also similar to AAK23160|CC1176 Metallo-beta-lactamase family protein from Caulobacter crescentus (317 aa), FASTA scores: opt: 286, E(): 1.8e-10, (33% identity in 291 aa overlap). And similar to other hypothetical proteins eg Q49744|B1937_C1_163 hypothetical 22.6 KDA protein (precursor) from Mycobacterium leprae (211 aa), FASTA scores: opt: 623, E(): 2.1e-31, (56.3% identity in 206 aa overlap); O27859|MTH1831 conserved protein from Methanothermobacter thermautotrophicus (307 aa), FASTA scores: opt: 268, E(): 2.3e-09, (28.35% identity in 307 aa overlap); etc. P9WGZ5 No conserved hypotheticals P9WGZ5 GO:0042779,GO:0008270,GO:0042781 3.1.26.11 P9WGZ5 NC_000962.3 Mycobrowser_v5 CDS 2710075 2710335 . + 0 Rv2412 rpsT Involved in translation mechanisms. Binds directly to 16S ribosomal RNA. 30S ribosomal protein S20 RpsT Rv2412, (MT2485, MTCY253.08c), len: 86 aa. rpsT, 30s ribosomal protein s20, equivalent to O33132|RS20_MYCLE|L0604|MLCL536.06 30S ribosomal protein S20 from Mycobacterium leprae (86 aa), FASTA scores: opt: 456, E(): 4.6e-24, (87.20% identity in 86 aa overlap). Also highly similar or similar to others e.g. Q9RDM3|RPST|SCC123.01 30S ribosomal protein S20 from Streptomyces coelicolor (88 aa), FASTA scores: opt: 363, E(): 7.1e-18, (70.95% identity in 86 aa overlap); Q9KD79|RPST|BH1339 ribosomal protein S20 (BS20) from Bacillus halodurans (91 aa), FASTA scores: opt: 252, E(): 1.8e-10, (49.4% identity in 85 aa overlap); P02378|RS20_ECOLI 30s ribosomal protein s20 from Escherichia coli (86 aa), FASTA scores: opt: 210, E(): 1e-07, (42.4% identity in 85 aa overlap); etc. Belongs to the S20P family of ribosomal proteins. P9WH41 No information pathways P66505 P9WH41 GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WH41 NC_000962.3 Mycobrowser_v5 CDS 2707333 2708310 . - 0 Rv2410c Rv2410c Function unknown Conserved protein Rv2410c, (MTCY253.10), len: 325 aa. Conserved protein, equivalent to Q49770|CAC30114|ML0606 conserved hypothetical protein from Mycobacterium leprae (325 aa), FASTA scores: opt: 1928, E(): 3.5e-117, (90.75% identity in 325 aa overlap). Also some similarity with other hypothetical proteins e.g. Q9RST2|DR2041 conserved hypothetical protein from Deinococcus radiodurans (316 aa), FASTA scores: opt: 329, E(): 5.3e-14, (32.4% identity in 318 aa overlap); C-terminus of Q9HUN7|PA4927 hypothetical protein from Pseudomonas aeruginosa (830 aa), FASTA scores: opt: 297, E(): 1.5e-11, (27.6% identity in 315 aa overlap); etc. P71733 No conserved hypotheticals P71733 P71733 NC_000962.3 Mycobrowser_v5 CDS 2710351 2711301 . - 0 Rv2413c Rv2413c Function unknown Conserved hypothetical protein Rv2413c, (MTCY253.07), len: 316 aa. Conserved hypothetical protein, highly similar to O33133|MLCL536.07c|ML0603|Q49756|G466975|B1937_F2_36 hypothetical 39.1 KDA protein from Mycobacterium leprae (389 aa), FASTA scores: opt: 1683, E(): 1.8e-88, (83.9% identity in 316 aa overlap). ML0603 is a putative lipoprotein with an N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein lipid attachment site that is not present in Rv2413c as this seems to be 73 aa shorter. Also some similarity with various proteins from other organisms e.g. Q9RDM2|SCC123.02c putative DNA-binding protein from Streptomyces coelicolor (336 aa), FASTA scores: opt: 792, E(): 6.1e-38, (42.4% identity in 316 aa overlap); Q9HX31|HOLA|PA3989 DNA polymerase III, delta subunit from Pseudomonas aeruginosa (345 aa), FASTA scores: opt: 173, E(): 0.0084, (25.4% identity in 307 aa overlap); etc. P71730 No conserved hypotheticals P71730 P71730 GO:0009360,GO:0006260,GO:0003887,GO:0003677 P71730 NC_000962.3 Mycobrowser_v5 CDS 2711332 2712876 . - 0 Rv2414c Rv2414c Function unknown Conserved hypothetical protein Rv2414c, (MTCY253.06), len: 514 aa. Conserved hypothetical protein, showing some similarity with come operon proteins 3 (COMEC or COME3) e.g. Q9RTB1|DR1854 putative competence protein COMEC/REC2 from Deinococcus radiodurans (755 aa), FASTA scores: opt: 311, E(): 8.2e-11, (27.3% identity in 538 aa overlap); P73100|come|SLL1929 come protein from Synechocystis sp. strain PCC 6803 (709 aa), FASTA scores: opt: 302, E(): 2.6e-10, (26.3% identity in 323 aa overlap) (no similarity on N-terminus); P39695|CME3_BACSU come operon protein 3 from Bacillus subtilis (776 aa), FASTA scores: opt: 273, E(): 1.4e-08, (25.2% identity in 282 aa overlap) (no similarity on N-terminus); etc. P71729 No conserved hypotheticals P71729 P71729 NC_000962.3 Mycobrowser_v5 CDS 2726193 2726780 . + 0 Rv2428 ahpC Involved in oxidative stress response. LPDC|Rv0462, DLAT|Rv2215, AHPD|Rv2429, and AHPC|Rv2428 constitute an NADH-dependent peroxidase and peroxynitrite reductase that provides protection against oxidative stress. Alkyl hydroperoxide reductase C protein AhpC (alkyl hydroperoxidase C) Rv2428, (MTCY428.18c), len: 195 aa. AhpC, alkyl hydroperoxide reductase C (see citations below), equivalent to other alkyl hydroperoxide reductases C mycobacterial proteins e.g. Q9CBF5|AHPC|ML2042 alkyl hydroperoxide reductase from Mycobacterium leprae (195 aa) FASTA scores: opt: 1183, E(): 2.6e-72, (88.20% identity in 195 aa overlap); O87323|AHPC from Mycobacterium marinum (195 aa), FASTA scores: opt: 1215, E(): 1.9e-74, (90.8% identity in 195 aa overlap); Q57413|AHPC|AVI-3 from Mycobacterium avium (195 aa), FASTA scores: opt: 1201, E(): 1.6e-73, (90.25% identity in 195 aa overlap). Also highly similar to others from other organisms e.g. Q9FBP5|AHPC alkyl hydroperoxide reductase from Streptomyces coelicolor (184 aa), FASTA scores: opt: 768, E(): 1.7e-44, (62.45% identity in 189 aa overlap); etc. P9WQB7 No virulence, detoxification, adaptation 2BMX Q7BHK8 P9WQB7 GO:0055114,GO:0004601,GO:0051920,GO:0006950,GO:0045454 1.11.1.15 INH P9WQB7 NC_000962.3 Mycobrowser_v5 CDS 2714124 2715332 . - 0 Rv2416c eis Acetylation, substrate unknown. Involved in intracellular survival. Possibly associated with the cell surface and secreted. Modulates cytokine secretion by host immune cells. Enhanced intracellular survival protein Eis, GCN5-related N-acetyltransferase Rv2416c, (MTCY253.04), len: 402 aa. Eis, enhanced intracellular survival gene (see citations below). Conserved hypothetical protein, contains GNAT (Gcn5-related N-acetyltransferase) domain in N-terminal part, similar to Q9F309|SCC80.10 hypothetical 44.7 KDA protein from Streptomyces coelicolor (413 aa), FASTA scores: opt: 382, E(): 1e-16, (31.45% identity in 407 aa overlap); Q9K4F4|SCD66.23 conserved hypothetical protein from Streptomyces coelicolor (418 aa), FASTA scores: opt: 238, E(): 1.3e-07, (36.5% identity in 364 aa overlap): and Q54238|G1139577|ORF5 hypothetical protein from Streptomyces griseus (416 aa), FASTA scores: opt: 237, E(): 1.5e-07, (34.0 identity in 423 aa overlap). Start changed since first submission (- 6 aa) (see Dahl et al., 2001; Wei et al., 2000; Vetting et al. 2005). P9WFK7 No virulence, detoxification, adaptation 3R1K P9WFK7 GO:0008152,GO:0008080 P9WFK7 NC_000962.3 Mycobrowser_v5 CDS 2715472 2716314 . - 0 Rv2417c Rv2417c Function unknown Conserved protein Rv2417c, (MTCY253.03), len: 280 aa. Conserved protein, highly similar to Q9RDL7|SCC123.07c hypothetical 29.2 KDA protein from Streptomyces coelicolor (281 aa), FASTA scores: opt: 579, E(): 3.6e-27, (38.3% identity in 274 aa overlap). Also some similarity with DEGV proteins or hypothetical proteins from other organisms, e.g. Q9RSY3|DR1986 from Deinococcus radiodurans (281 aa), FASTA scores: opt: 393, E(): 3.4e-16, (31.0% identity in 280 aa overlap); P32436|DEGV_BACSU from Bacillus subtilis (281 aa), FASTA scores: opt: 365, E(): 1.5e-14, (27.8% identity in 284 aa overlap); BAB41937|BAB46307|SA0704|SAV0749 Conserved hypothetical protein from Staphylococcus aureus strain Mu50 and N315 (288 aa), FASTA scores: opt: 371, E(): 7e-15, (28.85% identity in 281 aa overlap); etc. P9WP05 No conserved hypotheticals P67368 P9WP05 P9WP05 NC_000962.3 Mycobrowser_v5 CDS 2717128 2717799 . - 0 Rv2419c gpgP Involved in the biosynthesis of methylglucose lipopolysaccharide (MGLP). Catalyzes the dephosphorylation of glucosyl-3-phosphoglycerate (GPG) to glucosylglycerate (GG). [Catalytic activity: glucosyl-3-phosphoglycerate + H2O = glucosylglycerate + phosphate] Glucosyl-3-phosphoglycerate phosphatase GpgP Rv2419c, (MTCY428.28-MTCY253.01), len: 223 aa. gpgP, glucosyl-3-phosphoglycerate phosphatase (See Mendes et al., 2011). Contains PS00175 Phosphoglycerate mutase family phosphohistidine signature. Belongs to the phosphoglycerate mutase family. Enzyme activity inhibited by Co2+ and Cu2+ (See Mendes et al., 2011). P9WIC7 No intermediary metabolism and respiration P71724 P9WIC7 GO:0004619,GO:0008152 3.1.3.70 P9WIC7 NC_000962.3 Mycobrowser_v5 CDS 2717796 2718176 . - 0 Rv2420c Rv2420c Function unknown Conserved hypothetical protein Rv2420c, (MTCY428.27), len: 126 aa. Conserved hypothetical protein, equivalent to Q9CBZ9|ML1453 hypothetical protein from Mycobacterium leprae (129 aa), FASTA scores: opt: 681, E(): 1.6e-38, (87.0% identity in 123 aa overlap). Also highly similar to Q9RDK9|SCC123.15c hypothetical protein from Streptomyces coelicolor (148 aa), FASTA scores: opt: 447, E(): 5.8e-23, (52.7% identity in 129 aa overlap); and similar to others e.g. P54457|YQEL_BACSU hypothetical protein from Bacillus subtilis (118 aa), FASTA scores: opt: 318, E(): 1.8e-14, (37.3% identity in 110 aa overlap); Q9KD89|BH1328 hypothetical protein from Bacillus halodurans (117 aa), FASTA scores: opt: 296, E(): 5.1e-13, (37.6% identity in 109 aa overlap); etc. O86327 No conserved hypotheticals O86327 O86327 O86327 NC_000962.3 Mycobrowser_v5 CDS 2776975 2777391 . - 0 Rv2475c Rv2475c Function unknown Conserved protein Rv2475c, (MTV008.31c), len: 138 aa. Conserved protein, showing similarity with Q9L245|SC6D10.19c hypothetical 16.2 KDA protein from Streptomyces coelicolor (136 aa), FASTA scores: opt: 236, E(): 1.9e-09, (34.1% identity in 126 aa overlap). Also some similarity with AAK44393|Z97050|MTCI28_3 conserved hypothetical protein from Mycobacterium tuberculosis cosmid I (151 aa), FASTA scores: opt: 147, E(): 0.00025, (29.2% identity in 120 aa overlap). I6Y9E8 No conserved hypotheticals I6Y9E8 NC_000962.3 Mycobrowser_v5 CDS 3031040 3031537 . - 0 Rv2719c Rv2719c Unknown Possible conserved membrane protein Rv2719c, (MTCY05A6.40c), len: 165 aa. Possible conserved membrane protein, equivalent to Q49846|ML1004|B2235_C3_243 possible conserved membrane protein from Mycobacterium leprae (164 aa), FASTA scores: opt: 486, E(): 4e-21, (55.2% identity in 163 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). I6YA32 No cell wall and cell processes O07218 I6YA32 GO:0016998 NC_000962.3 Mycobrowser_v5 CDS 2718173 2718808 . - 0 Rv2421c nadD Involved in NAD biosynthesis; catalyzes the reversible adenylation of nicotinate mononucleotide [catalytic activity: ATP + nicotinate ribonucleotide = diphosphate + deamido-NAD(+)]. Probable nicotinate-nucleotide adenylyltransferase NadD (deamido-NAD(+) pyrophosphorylase) (deamido-NAD(+) diphosphorylase) (nicotinate mononucleotide adenylyltransferase) (NAMN adenylyltransferase) Rv2421c, (MT2494, MTCY428.26), len: 211 aa. Probable nadD, nicotinate-nucleotide adenylyltransferase , equivalent to Q9CBZ8|NADD_MYCLE|ML1454 probable nicotinate-nucleotide adenylyltransferase from Mycobacterium leprae (214 aa), FASTA scores: opt: 1125, E(): 2.7e-66, (80.2% identity in 212 aa overlap). Also highly similar to Q9RDK7|NADD_STRCO probable nicotinate-nucleotide adenylyltransferase from Streptomyces coelicolor (188 aa), FASTA scores: opt: 855, E(): 9.8e-49, (66.5% identity in 194 aa overlap); and similar to others e.g. P54455|NADD_BACSU from Bacillus subtilis (189 aa), FASTA scores: opt: 351, E(): 7e-16, (36.1% identity in 191 aa overlap); etc. Belongs to the NadD family. P9WJJ5 No intermediary metabolism and respiration O86328 P9WJJ5 GO:0009435,GO:0004515,GO:0005524 2.7.7.18 P9WJJ5 NC_000962.3 Mycobrowser_v5 CDS 2730557 2732749 . - 0 Rv2435c Rv2435c Generates 3,'5'-cyclic (a/G)mp and diphosphate (or pyrophosphate) from (a/G)TP. Probable cyclase (adenylyl- or guanylyl-)(adenylate- or guanylate-) Rv2435c, (MTCY428.11), len: 730 aa. Probable cyclase (adenylyl- or guanylyl-cyclase; EC 4.6.1.1 or 4.6.1.2 respectively); C-terminal domain (aa 500-730) similar to domain at C-terminus of a series of adenylate/guanylate cyclases e.g. O30820|CYA AAK45931|MT1661 from Mycobacterium tuberculosis (443 aa) FASTA scores: opt: 446, E(): 1.3e-19, (30.55% identity in 301 aa overlap); BAB50179|MLL3242 cyclase (adenylyl or guanylyl) from Rhizobium loti (356 aa), FASTA scores: opt: 372, E(): 3.4e-15, (28.75% identity in 219 aa overlap); etc. Belongs to adenylyl cyclase class-4/guanylyl cyclase family. P71914 No intermediary metabolism and respiration P71914 P71914 GO:0016021,GO:0016849,GO:0004871,GO:0007165,GO:0009190 4.6.1.- P71914 NC_000962.3 Mycobrowser_v5 CDS 2734376 2734795 . + 0 Rv2437 Rv2437 Function unknown Conserved transmembrane protein Rv2437, (MTCY428.09c), len: 139 aa. Conserved transmembrane protein, with some similarity to conserved hypothetical proteins e.g. O06539|RV1139C|MTCI65.06c from Mycobacterium tuberculosis (166 aa); AAK45430|MT1172 from Mycobacterium tuberculosis (124 aa), FASTA scores: opt: 166, E(): 0.00013, (35.7% identity in 112 aa overlap); BAB48937|Mlr1600 from Rhizobium loti (222 aa), FASTA scores: opt: 163 ,E(): 0.00033, (28.1% identity in 121 aa overlap); etc. Contains membrane spanning regions. P71912 No cell wall and cell processes P71912 P71912 GO:0016021,GO:0004671,GO:0006481 P71912 NC_000962.3 Mycobrowser_v5 CDS 2745767 2746138 . - 0 Rv2446c Rv2446c Unknown Probable conserved integral membrane protein Rv2446c, (MTV008.02c), len: 123 aa. Probable conserved integral membrane protein, highly similar to Q9CBY9|ML1470 conserved membrane protein from Mycobacterium leprae (123 aa), FASTA scores: opt: 468, E(): 6.7e-23, (66.65% identity in 108 aa overlap). Also similar to Q9L1G5|SCC88.24c putative membrane protein from Streptomyces coelicolor (118 aa), FASTA scores: opt: 130, E(): 0.13, (37.2% identity in 86 aa overlap); and some similarity to O06852|Y13070 hypothetical Streptomyces coelicolor gene also between fpgs and ndk genes (see citation below) (117 aa), FASTA scores: opt: 128, E(): 0.17, (36.0% identity in 86 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53173 No cell wall and cell processes O53173 NC_000962.3 Mycobrowser_v5 CDS 2736709 2736987 . + 0 Rv2438A Rv2438A Function unknown Conserved hypothetical protein Rv2438A, len: 92 aa. Conserved hypothetical protein, showing few similarity with various enzymes e.g. part of O83441|VAA1_TREPA|ATPA1|TP0426 V-type ATP synthase alpha chain 1 from Treponema pallidum (589 aa), FASTA scores: opt: 110, E(): 1.5, (40.3% identity in 72 aa overlap); N-terminus of O95178|NIGM_HUMAN NADH-ubiquinone oxidoreductase AGGG subunit precursor from Homo sapiens (105 aa), FASTA scores: opt: 109, E(): 1.5, (35.5% identity in 62 aa overlap); N-terminus of Q9HJ76|TA1096 probable glycerol kinase from Thermoplasma acidophilum (488 aa); etc. Q79FD9 No conserved hypotheticals Q79FD9 NC_000962.3 Mycobrowser_v5 CDS 2753018 2753623 . - 0 Rv2453c mobA Involved in molybdenum cofactor biosynthesis. LINKS a guanosine 5'-phosphate to molydopterin (MPT) forming molybdopterin guanine dinucleotide (MGD). Probable molybdopterin-guanine dinucleotide biosynthesis protein A MobA Rv2453c, (MT2528, MTV008.09c), len: 201 aa. Probable mobA, molybdopterin-guanine dinucleotide biosynthesis protein A, similar to others e.g. Q9F8G7 from Carboxydothermus hydrogenoformans (224 aa), FASTA scores: opt: 249, E(): 3.9e-08, (30.6% identity in 173 aa overlap); P95645|MOBA_RHOSH|mob|Y09560 from Rhodobacter sphaeroides (199 aa), FASTA scores: opt: 240, E(): 1.2e-07, (33.9% identity in 186 aa overlap); Q9X7K0|MOBA_RHOCA from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (191 aa), FASTA scores: opt: 217, E(): 2.9e-06, (37.4% identity in 123 aa overlap); etc. Belongs to the MobA family. P9WJQ9 No intermediary metabolism and respiration P9WJQ9 GO:0006777,GO:0005737,GO:0005525 P9WJQ9 NC_000962.3 Mycobrowser_v5 CDS 2854938 2857781 . - 0 Rv2531c Rv2531c Unknown. Could be an ornithine/arginine/lysine decarboxylase involved in the biosynthesis of spermidine from arginine. Probable amino acid decarboxylase Rv2531c, (MTCY159.25), len: 947 aa. Probable amino acid decarboxylase, equivalent to Q9CCR8|adi|ML0524 putative amino acid decarboxylase from Mycobacterium leprae (950 aa), FASTA scores: opt: 5426, E(): 0, (86.45% identity in 951 aa overlap). Also similar to other amino acid decarboxylases (but longer in N-terminus) e.g. Q9I2S7|PA1818 probable ORN/ARG/LYS amino acid decarboxylase from Pseudomonas aeruginosa (751 aa), FASTA scores: opt: 434, E(): 2.5e-19, (29.15% identity in 738 aa overlap); Q9CML3|SPEF|PM0806 ornithine decarboxylase from Pasteurella multocida (720 aa), FASTA scores: opt: 402, E(): 2.4e-17, (24.85% identity in 752 aa overlap); P21169|DCOR_ECOLI|spec|B2965|BAB37264|ECS3841|AAG58096 ornithine decarboxylase isozyme (constitutive enzyme) from Escherichia coli strain K12 (711 aa), FASTA scores: opt: 396, E(): 5.6e-17, (28.0% identity in 646 aa overlap); P44317|DCOR_HAEIN|SPEF|HI0591 ornithine decarboxylase from Haemophilus influenzae (720 aa), FASTA scores: opt: 393, E(): 8.8e-17, (25.05% identity in 743 aa overlap) ; etc. Seems to belong to family 1 of ornithine, lysine, and arginine decarboxylases. Note that previously known as adi. I6X4K0 No intermediary metabolism and respiration Q7D6Y7 I6X4K0 GO:0030170,GO:0016829 4.1.1.- NC_000962.3 Mycobrowser_v5 CDS 2742123 2744984 . - 0 Rv2444c rne Thought to be involved in several cellular process. Possible ribonuclease E Rne Rv2444c, (MTCY428.02), len: 953 aa. Possible rne, ribonuclease E, highly similar to others e.g. Q9CBZ1|ML1468 possible ribonuclease from Mycobacterium leprae (924 aa), FASTA scores: opt: 3713, E(): 2.4e-174, (74.2% identity in 966 aa overlap); Q9SI08|AT2G04270 putative ribonuclease E from Arabidopsis thaliana (502 aa), FASTA scores: opt: 674, E(): 7.5e-26, (31.2% identity in 410 aa overlap); etc. Similar at C-terminal end to P21513|RNE_ECOLI|ams|HMP1|B1084 ribonuclease E (RNASE E) from Escherichia coli strain K12 (1061 aa), FASTA scores: opt: 554, E(): 9.9e-20, (37.8% identity in 386 aa overlap). Also similar in medium part to several cytoplasmic axial filament proteins e.g. Q9HVU4|CAFA|PA4477 from Pseudomonas aeruginosa (485 aa), FASTA scores: opt: 664, E(): 2.3e-25, (42.8% identity in 418 aa overlap); etc. Equivalent to AAK46818 from Mycobacterium tuberculosis strain CDC1551 (621 aa) but longer 332 aa in N-terminal part. Seems to belong to the RNE family. P71905 No information pathways P71905 P71905 GO:0006396,GO:0005737,GO:0004540,GO:0003723 3.1.-.- P71905 NC_000962.3 Mycobrowser_v5 CDS 2745314 2745724 . - 0 Rv2445c ndkA Major role in the synthesis of nucleoside triphosphates other than ATP [catalytic activity: ATP + nucleoside diphosphate = ADP + nucleoside triphosphate]. Probable nucleoside diphosphate kinase NdkA (NDK) (NDP kinase) (nucleoside-2-P kinase) Rv2445c, (MTV008.01c, MTCY428.01), len: 136 aa. Probable ndkA (alternate gene name: ndk), nucleoside diphosphate kinase, equivalent to Q9CBZ0|NDK|ML1469 from Mycobacterium leprae (136 aa), FASTA scores: opt: 762, E(): 1.5e-42, (87.4% identity in 135 aa overlap); and O85501|NDK from Mycobacterium smegmatis (139 aa), FASTA scores: opt: 714, E(): 1.9e-39, (80.7% identity in 135 aa overlap). Also highly similar to others e.g. P50589|NDK_STRCO from Streptomyces coelicolor (137 aa), FASTA scores: opt: 535, 6.8e-28, (60.3% identity in 136 aa overlap); O29491|NDK_ARCFU|AF0767 from Archaeoglobus fulgidus (151 aa), FASTA scores: opt: 521, E(): 5.9e-27, (58.0% identity in 131 aa overlap); P31103|NDK_BACSU from Bacillus subtilis (151 aa), FASTA scores: opt: 515, E(): 1.4e-26, (56.5% identity in 131 aa overlap); etc. Belongs to the NDK family. Ppk2|Rv3232c and NdkA|Rv2445c interact (See Sureka et al., 2009). P9WJH7 No intermediary metabolism and respiration 1K44 P84284 P9WJH7 GO:0006241,GO:0006183,GO:0006228,GO:0005737,GO:0046872,GO:0004550,GO:0005524 2.7.4.6 P9WJH7 NC_000962.3 Mycobrowser_v5 CDS 2750313 2751572 . - 0 Rv2449c Rv2449c Unknown Conserved protein Rv2449c, (MTV008.05c), len: 419 aa. Conserved protein, highly similar to hypothetical proteins e.g. P95139|Rv2953|MTCY349.37c from M. tuberculosis (418 aa), FASTA scores: opt: 1829, E(): 4.7e-103, (67.3% identity in 419 aa overlap); AAK47353|MT3027 from Mycobacterium tuberculosis strain CDC1551 (418 aa), FASTA score: opt: 1829, E(): 4.7e-103, (67.3 identity in 419 aa overlap); Q9CD87|ML0129 from Mycobacterium leprae (418 aa), FASTA scores: opt: 1727, E(): 6.8e-97, (65.45% identity in 414 aa overlap); etc. O53176 No conserved hypotheticals O53176 O53176 GO:0003824,GO:0008152 O53176 NC_000962.3 Mycobrowser_v5 CDS 2773178 2773564 . + 0 Rv2470 glbO Oxygen carrier, involved in oxygen transport. Globin (oxygen-binding protein) GlbO Rv2470, (MTV008.26), len: 128 aa. glbO, globin-like protein, highly similar to Q9CC59|GLBO|ML1253 hemoglobin-like (oxygen carrier) from Mycobacterium leprae (128 aa), FASTA scores: opt: 767, E(): 4e-47, (88.1% identity in 126 aa overlap); Q9X7B3|MLCB1610.14c putative globin from Mycobacterium leprae (131 aa); Q9L250|SC6D10.14 putative globin from Streptomyces coelicolor (137 aa), FASTA scores: opt: 466, E(): 5.7e-26, (53.6% identity in 125 aa overlap). Also similar to O31607 YJBI protein from Bacillus subtilis (132 aa), FASTA scores: opt: 294, E(): 6.6e-14; (39.85% identity in 128 aa overlap). Could belong to protozoan/cyanobacterial globin family protein. P9WN23 No intermediary metabolism and respiration 1NGK,2QRW P0A595 P9WN23 GO:0019825,GO:0015671,GO:0005344,GO:0005886,GO:0020037 P9WN23 NC_000962.3 Mycobrowser_v5 CDS 2816885 2818474 . - 0 Rv2502c accD1 Involved in fatty acid metabolism. Probable acetyl-/propionyl-CoA carboxylase (beta subunit) AccD1 Rv2502c, (MTCY07A7.08c), len: 529 aa. Probable accD1, acetyl-/propionyl-CoA carboxylase (beta subunit) , similar, but with N-terminus shorter, to Q9L077|ACCD1 from Streptomyces coelicolor (538 aa), FASTA scores: opt: 2747, E(): 1.9e-159, (77.9% identity in 516 aa overlap). Also similar to others e.g. AAK24141|CC2170 from Caulobacter crescentus (530 aa), FASTA scores: opt: 2413, E(): 3.8e-139, (69.4% identity in 529 aa overlap); BAB54131|MLL7731 from Rhizobium loti (537 aa), FASTA scores: opt: 2399, E(): 2.7e-138, (67.4% identity in 527 aa overlap); etc. Could belong to the ACCD/PCCB family. I6YDK7 No lipid metabolism O06165 I6YDK7 GO:0004658 6.4.1.3 I6YDK7 NC_000962.3 Mycobrowser_v5 CDS 4149591 4149911 . - 0 Rv3706c Rv3706c Function unknown Conserved hypothetical proline rich protein Rv3706c, (MTV025.054c), len: 106 aa. Conserved ypothetical pro-rich protein, similar to upstream ORF Rv3705A (129 aa), and AAK48176|MT3808.1 hypothetical 13.0 KDA protein from Mycobacterium tuberculosis strain CDC1551 (129 aa), FASTA scores: opt: 245, E(): 4.4e-06, (40.7% identity in 113 aa overlap). I6X849 No conserved hypotheticals I6X849 I6X849 NC_000962.3 Mycobrowser_v5 CDS 2784657 2785643 . - 0 Rv2479c Rv2479c Required for the transposition of the insertion element IS6110. Probable transposase Rv2479c, (MTV008.35c), len: 328 aa. Probable transposase for IS6110. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2480c and Rv2479c, the sequence UUUUAAAG (directly upstream of Rv2479c) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (- 18 aa). P9WKH9 No insertion seqs and phages P0C5G8 P9WKH9 GO:0015074,GO:0006310,GO:0032196,GO:0003677 P9WKH9 NC_000962.3 Mycobrowser_v5 CDS 2753625 2754746 . - 0 Rv2454c Rv2454c Function unknown; probably involved in cellular metabolism. Probable oxidoreductase (beta subunit) Rv2454c, (MTV008.10c), len: 373 aa. Probable oxidoreductase, beta subunit, similar to Q9F2W7|SCD20.12c putative oxidoreductase from Streptomyces coelicolor (352 aa), FASTA scores: opt: 1461, E(): 6.4e-85, (65.3% identity in 343 aa overlap) alias Q9RKS5|STAH10.34c putative oxidoreductase beta-subunit from Streptomyces coelicolor (350 aa), FASTA scores: opt: 1429, E(): 6.7e-83, (64.0% identity in 342 aa overlap); and similar in part to others e.g. Q9Z5X3 ferredoxin oxidoreductase B-subunit from Frankia sp. (346 aa), FASTA scores: opt: 1143, E(): 7.5e-65, (51.2% identity in 336 aa overlap); BAB21495|KORB ferredoxin oxidoreductase beta subunit from Hydrogenobacter thermophilus TK-6 (295 aa), FASTA scores: opt: 682, E(): 8.3e-36, (48.25% identity in 201 aa overlap); etc. Note that the upstream ORF (MTV008.11c|Rv2455c) is possibly an oxidoreductase alpha subunit. O53181 No intermediary metabolism and respiration O53181 O53181 GO:0016491,GO:0030976,GO:0055114 1.-.-.- O53181 NC_000962.3 Mycobrowser_v5 CDS 2754743 2756704 . - 0 Rv2455c Rv2455c Function unknown; probably involved in cellular metabolism. Probable oxidoreductase (alpha subunit) Rv2455c, (MTV008.11c), len: 653 aa. Probable oxidoreductase, alpha subunit, similar to others e.g. Q9F2W6|SCD20.13c putative oxidoreductase from Streptomyces coelicolor (645 aa), FASTA scores: opt: 2017, E(): 1e-111, (66.45% identity in 617 aa overlap) alias Q9RKS4|STAH10.35c putative oxidoreductase alpha-subunit from Streptomyces coelicolor (630 aa), FASTA scores: opt: 2008, E(): 3.4e-111, (66.45% identity in 614 aa overlap); Q9YA13|APE2126 long hypothetical 2-oxoacid--ferredoxin oxidoreductase alpha chain from Aeropyrum pernix (644 aa) FASTA scores: opt: 687, E(): 4.6e-33, (33.35% identity in 441 aa overlap); etc. Note that the downstream ORF (MTV008.10c|Rv2454c) is possibly an oxidoreductase beta subunit. O53182 No intermediary metabolism and respiration O53182 O53182 GO:0016903,GO:0055114 1.-.-.- O53182 NC_000962.3 Mycobrowser_v5 CDS 2756936 2758192 . - 0 Rv2456c Rv2456c Thought to be involved in transport of undetermined substrate (possibly sugar) across the membrane. Responsible for the translocation of the substrate across the membrane. Probable conserved integral membrane transport protein Rv2456c, (MTV008.12c), len: 418 aa. Probable conserved integral membrane transport protein, involved in a efflux system, weakly similar to many e.g. Q9RUR0|YD22_DEIRA|DR1322 putative sugar efflux transporter from Deinococcus radiodurans (389 aa), FASTA scores: opt: 224, E(): 8.4e-06, (24.45% identity in 409 aa overlap); Q9UYY0|PAB0913 multidrug resistance protein from Pyrococcus abyssi (410 aa), FASTA scores: opt: 210, E(): 5.6e-05, (21.8% identity in 408 aa overlap); etc. Contains PS00216 Sugar transport proteins signature 1. P9WJX1 No cell wall and cell processes O53183 P9WJX1 GO:0005886,GO:0055085,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2785592 2785918 . - 0 Rv2480c Rv2480c Thought to be required for the transposition of the insertion element IS6110. Possible transposase for insertion sequence element IS6110 (fragment) Rv2480c, (MTV008.36c), len: 108 aa. Putative Transposase for IS6110 (fragment). Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2480c and Rv2479c, the sequence UUUUAAAG (directly upstream of Rv2479c) maybe responsible for such a frameshifting event (see McAdam et al., 1990). P9WKH5 No insertion seqs and phages P0C5G9 P9WKH5 GO:0004803,GO:0006313,GO:0003677 P9WKH5 NC_000962.3 Mycobrowser_v5 CDS 2830877 2831338 . - 0 Rv2514c Rv2514c Function unknown Conserved hypothetical protein Rv2514c, (MTCY07A7.20c), len: 153 aa. Conserved hypothetical protein, showing some similarity to Q9PG05|XF0497 hypothetical protein from Xylella fastidiosa (155 aa), FASTA scores: opt: 215, E(): 1.4e-07, (30.6% identity in 160 aa overlap). I6Y0Y0 No conserved hypotheticals I6Y0Y0 I6Y0Y0 NC_000962.3 Mycobrowser_v5 CDS 2759779 2760687 . + 0 Rv2458 mmuM Catalyzes methyl transfer from S-methylmethionine or S-adenosylmethionine (less efficient) to homocysteine, selenohomocysteine and less efficiently selenocysteine [catalytic activity: S-adenosyl-L-methionine + L-homocysteine = S-adenosyl-L-homocysteine + L-methionine]. Probable homocysteine S-methyltransferase MmuM (S-methylmethionine:homocysteine methyltransferase) (cysteine methyltransferase) Rv2458, (MTV008.14), len: 302 aa. Probable mmuM, homocysteine S-methyltransferase, equivalent to Q9CBY5|ML1478 possible transferase from Mycobacterium leprae (293 aa), FASTA scores: opt: 1507, E(): 2.7e-86, (78.85% identity in 293 aa overlap). Also similar to others e.g. Q47690|MMUM_ECOLI|B0261 homocysteine S-methyltransferase from Escherichia coli strain K12 (310 aa), FASTA scores: opt: 863, E(): 2.4e-46, (47.65% identity in 298 aa overlap); Q9FUM7 homocysteine S-methyltransferase-4 from Zea mays (Maize) (342 aa), FASTA scores: opt: 324, E(): 6.8e-13, (44.45% identity in 306 aa overlap); Q9LUI7|HMT3 cysteine methyltransferase from Arabidopsis thaliana (Mouse-ear cress) (347 aa), FASTA scores: opt: 312, E(): 3.8e-12, (41.85% identity in 313 aa overlap); etc. Identical to AAK46833|MT2533 homocysteine S-methyltransferase from Mycobacterium tuberculosis strain CDC1551 (302 aa). O53185 No intermediary metabolism and respiration O53185 O53185 GO:0008898 2.1.1.10 O53185 NC_000962.3 Mycobrowser_v5 CDS 2760854 2762380 . + 0 Rv2459 Rv2459 Thought to be involved in a transport system across the membrane (perhaps drug transport): responsible for the translocation of the substrate across the membrane. Probable conserved integral membrane transport protein Rv2459, (MTV008.15), len: 508 aa. Probable conserved integral membrane transport protein, member of major facilitator superfamily (MFS) possibly involved in drug transport, highly similar to many efflux proteins e.g. Q9RL22|SC5G9.04c putative transmembrane efflux protein from Streptomyces coelicolor (489 aa), FASTA scores: opt: 788, E(): 1.3e-38, (34.45% identity in 412 aa overlap); Q9I428|PA1316 probable MFS transporter from Pseudomonas aeruginosa (513 aa), FASTA scores: opt: 782, E(): 3.1e-38, (32.75% identity in 519 aa overlap); P39886|TCMA_STRGA tetracenomycin C resistance and export protein from Streptomyces glaucescens (538 aa), FASTA scores: opt: 752, E(): 1.8e-36, (31.7% identity in 511 aa overlap); etc. Also highly similar to AAK46687|MT2395 drug transporter from Mycobacterium tuberculosis strain CDC1551 (537 aa), FASTA scores: opt: 1396, E(): 5.6e-74, (44.45% identity in 504 aa overlap); and P71879|Rv2333c|MTCY3G12.01 probable conserved integral membrane transport protein from Mycobacterium tuberculosis strain H37Rv (537 aa), FASTA scores: opt: 1385, E(): 2.5e-73, (44.25% identity in 504 aa overlap). P9WJW9 No cell wall and cell processes O53186 P9WJW9 GO:0005886,GO:0055085,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2762531 2763175 . - 0 Rv2460c clpP2 CLP cleaves peptides in various proteins in a process that requires ATP hydrolysis. CLP may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. Probable ATP-dependent CLP protease proteolytic subunit 2 ClpP2 (endopeptidase CLP 2) Rv2460c, (MT2535, MTV008.16c), len: 214 aa. Probable clpP2, ATP-dependent clp protease proteolytic subunit 2, equivalent to Q9CBY4|CLP2_MYCLE ATP-dependent CLP protease proteolytic subunit from Mycobacterium leprae (214 aa). Also highly similar to others e.g. Q9ZH58|CLPP2 from Streptomyces coelicolor (236 aa), FASTA scores: opt: 918, E(): 2.1e-50, (66.35% identity in 214 aa overlap); O67357|CLPP_AQUAE|AQ_1339 from Aquifex aeolicus (201 aa), FASTA scores: opt: 680, E(): 1.4e-35, (52.0% identity in 194 aa overlap); P43867|CLPP_HAEIN from Haemophilus influenzae (193 aa), FASTA scores: opt: 662, E(): 1.8e-34, (53.35% identity in 193 aa overlap); etc. Contains PS00381 Endopeptidase Clp serine active site. Also similar to upstream ORF Rv2461c|MTV008.17c|clpP1 (200 aa), FASTA score: (48.3% identity in 172 aa overlap). Belongs to peptidase family S14, also known as ClpP family. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P9WPC3 No intermediary metabolism and respiration P63783 P9WPC3 GO:0005737,GO:0005515,GO:0006508,GO:0004252,GO:0005524 3.4.21.92 P9WPC3 NC_000962.3 Mycobrowser_v5 CDS 2786575 2786898 . - 0 Rv2481c Rv2481c Unknown Hypothetical protein Rv2481c, (MTV008.37c), len: 107 aa. Hypothetical unknown protein. O53206 No conserved hypotheticals O53206 O53206 NC_000962.3 Mycobrowser_v5 CDS 2763891 2765291 . - 0 Rv2462c tig Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation. Probable trigger factor (TF) protein Tig Rv2462c, (MTV008.18c), len: 466 aa. Probable tig, trigger factor (TF), a chaperone protein, equivalent to Q9CBY2|ML1481 possible molecular chaperone from Mycobacterium leprae (469 aa), FASTA scores: opt: 2171, E(): 7.2e-113, (70.1% identity in 468 aa overlap). Also similar to oyher trigger factors from several organisms e.g. Q9F314|SCC80.05c from Streptomyces coelicolor (468 aa), FASTA scores: opt: 1224, E(): 1.7e-60, (41.8% identity in 469 aa overlap); Q9K8F3|TIG_BACHD from Bacillus halodurans (431 aa), FASTA scores: opt: 675, E(): 3.6e-30, (28.5% identity in 421 aa overlap); P22257|TIG_ECOLI from Escherichia coli (432 aa), FASTA scores: opt: 493, E(): 4.2e-20, (23.35% identity in 433 aa overlap); etc. Belongs to the FKBP-type PPIase family, TIG subfamily. P9WG55 No cell wall and cell processes O53189 P9WG55 GO:0051301,GO:0003755,GO:0006457,GO:0015031,GO:0007049 P9WG55 NC_000962.3 Mycobrowser_v5 CDS 2766859 2767665 . - 0 Rv2464c Rv2464c Hydrolyses DNA (this enzyme may play a significant role in processes leading to recovery from mutagenesis and/or cell death by alkylating agents). Possible DNA glycosylase Rv2464c, (MT2539, MTV008.20c), len: 268 aa. Possible DNA glycosylase, showing some similarity to several other DNA glycosylases e.g. Q9F308|SCC80.11c putative DNA repair hydrolase (fragment) from Streptomyces coelicolor (306 aa), FASTA scores: opt: 894, E(): 6.1e-51, (51.05% identity in 282 aa overlap); O50606|MUTM|FPG_THETH formamidopyrimidine-DNA glycosylase from Thermus aquaticus (267 aa), FASTA scores: opt: 342, E(): 4.6e-15, (32.4% identity in 250 aa overlap); Q9RCW5|SCM10.34c putative formamidopyrimidine-DNA glycosylase from Streptomyces coelicolor (287 aa), FASTA scores: opt: 321, E(): 1.1e-13, (29.35% identity in 259 aa overlap); etc. Identical to AAK46839|MT2539 formamidopyrimidine-DNA glycosylase from Mycobacterium tuberculosis strain CDC1551. Also similar to other Mycobacterium tuberculosis DNA glycosylases e.g. MTCY71.37 (32.9% identity in 277 aa overlap). Belongs to the FPG family. P9WNB9 No information pathways P64158 P9WNB9 GO:0006284,GO:0003684,GO:0016799,GO:0006289,GO:0008270,GO:0003906 4.2.99.18,3.2.2.- P9WNB9 NC_000962.3 Mycobrowser_v5 CDS 2867124 2867786 . + 0 Rv2544 lppB Unknown Probable conserved lipoprotein LppB Rv2544, (MTCY159.12c), len: 220 aa. Probable lppB, conserved lipoprotein, highly similar to downstream ORF P95010|MTCY159.13c|LPPA|Rv2543|MTCY159.13 putative lipoprotein LPPA from Mycobacterium tuberculosis (219 aa), FASTA scores: opt: 1242, E(): 4.8e-72, (87.15% identity in 218 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. P9WK79 No cell wall and cell processes P9WK79 GO:0005886 P9WK79 NC_000962.3 Mycobrowser_v5 CDS 4095540 4096115 . - 0 Rv3657c Rv3657c Unknown Possible conserved alanine rich membrane protein Rv3657c, (MTV025.005c), len: 191 aa. Possible conserved membrane protein, rich in ala residues, similar to Q9X919|SCH5.17c putative integral membrane protein from Streptomyces coelicolor (267 aa), FASTA scores: opt: 324, E(): 4.7e-12, (40.9% identity in 154 aa overlap). O69625 No cell wall and cell processes O69625 O69625 O69625 NC_000962.3 Mycobrowser_v5 CDS 2768261 2768884 . - 0 Rv2466c Rv2466c Function unknown. Seems regulated by sigh (Rv3223c product). Conserved protein Rv2466c, (MTV008.22c), len: 207 aa. Conserved protein (see citation below), equivalent to Q9CBY0|ML1485 hypothetical protein from Mycobacterium leprae (207 aa), FASTA scores: opt: 1154, E(): 1.1e-67, (80.6% identity in 206 aa overlap). Also highly similar to Q9L201|SC8E4A.04c hypothetical protein from Streptomyces coelicolor (216 aa), FASTA scores: opt: 789, E(): 4.6e-44, (57.9% identity in 213 aa overlap). Also similar to AAK46628|MT2344 hypothetical protein from Mycobacterium tuberculosis strain CDC1551 (230 aa), FASTA scores: opt: 324, E(): 6.1e-14, (30.4% identity in 194 aa overlap). Contains PS00195 Glutaredoxin active site. O53193 No conserved hypotheticals O53193 O53193 GO:0015035,GO:0030288 O53193 NC_000962.3 Mycobrowser_v5 CDS 3011399 3011767 . - 0 Rv2694c Rv2694c Function unknown Conserved protein Rv2694c, (MTCY05A6.15c), len: 122 aa. Conserved protein, highly similar in part to SC2E9.14 hypothetical 16.9 KDA protein from Streptomyces coelicolor (154 aa), FASTA scores: opt: 299, E(): 1.9e-13, (41.05% identity in 117 aa overlap. Equivalent to AAK47083 from Mycobacterium tuberculosis strain CDC1551 (157 aa) but shorter 35 aa. O07196 No conserved hypotheticals O07196 NC_000962.3 Mycobrowser_v5 CDS 2768986 2771571 . + 0 Rv2467 pepN Aminopeptidase with broad substrate specificity to several peptides (could preferentially cleave leucine, arginine and lysine in peptide-bond-containing substrates). Probable aminopeptidase N PepN (Lysyl aminopeptidase) (LYS-AP) (alanine aminopeptidase) Rv2467, (MTV008.23), len: 861 aa. Probable pepN, aminopeptidase N, equivalent to Q9CBX9|ML1486 probable aminopeptidase from Mycobacterium leprae (862 aa), FASTA scores: opt: 4751,E(): 0, (83.3% identity in 862 aa overlap). Also highly similar to others e.g. Q11010|AMPN_STRLI|PEPN from Streptomyces lividans (857 aa), FASTA scores: opt: 2839, E(): 1.8e-170, (53.25% identity in 864 aa overlap); Q9L1Z2|PEPN from Streptomyces coelicolor (857 aa), FASTA scores: opt: 2834, E(): 3.8e-170, (53.1% identity in 864 aa overlap); P37896|AMPN_LACDL|PEPN from Lactobacillus delbrueckii (subsp. lactis) (842 aa), FASTA scores: opt: 719, E(): 2.4e-37, (31.65% identity in 439 aa overlap); etc. Contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. Belongs to peptidase family M1 (zinc metalloprotease), also known as the PEPN subfamily. Note that previously known as pepD. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). L7N655 No intermediary metabolism and respiration Q7D736 L7N655 GO:0008237,GO:0006508,GO:0008270,GO:0004177 3.4.11.2 L7N655 NC_000962.3 Mycobrowser_v5 CDS 2771644 2772147 . - 0 Rv2468c Rv2468c Function unknown Conserved protein Rv2468c, (MTV008.24c), len: 167 aa. Conserved protein, highly similar to Mycobacterium leprae hypothetical proteins Q9CC58|ML1255 (163 aa), FASTA scores: opt: 859, E(): 1.6e-49, (81.2% identity in 165 aa overlap) and Q9X7B5|MLCB1610.16 (169 aa), FASTA scores: opt: 859, E(): 1.6e-49, (81.2% identity in 165 aa overlap). Also weak similarity with Q9X8D7|SCE39.14c putative GntR-family regulator from Streptomyces coelicolor (243 aa), FASTA scores: opt: 116, E(): 1.3, (30.1% identity in 156 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WLA7 No conserved hypotheticals P9WLA7 P9WLA7 NC_000962.3 Mycobrowser_v5 CDS 2813173 2813730 . - 0 Rv2499c Rv2499c Function unknown Possible oxidase regulatory-related protein Rv2499c, (MTCY07A7.05c), len: 185 aa. Possible oxidase regulatory-related protein, similar to many maoC monoamine oxidase regulatory protein e.g. Q9RUZ1|DR1239 MAOC-related protein from Deinococcus radiodurans (160 aa), FASTA scores: opt: 519, E(): 7.6e-28, (58.1% identity in 148 aa overlap); BAB48392|MLR0905 Probable monoamine oxidase regulatory protein from Rhizobium loti (Mesorhizobium loti) (150 aa), FASTA scores: opt: 480, E(): 2.9e-25, (49.0% identity in 149 aa overlap); Q9HN18|MAOC1|VNG2290G monoamine oxidase regulatory-like from Halobacterium sp. strain NRC-1 (208 aa), FASTA scores: opt: 419, E(): 4.6e-21, (45.6% identity in 158 aa overlap); P77455|MAOC_ECOLI|PAAZ|B1387 MaoC protein (Phenylacetic acid degradation protein paaZ) from Escherichia coli strain K12 (681 aa), FASTA scores: opt: 252, E(): 1.9e-09, (36.0% identity in 172 aa overlap); etc. But also similar to other proteins with different putative functions e.g. Q9HRM9|MAOC2|VNG0626G molybdenum cofactor biosynthesis protein from Halobacterium sp strain NRC-1 (157 aa), FASTA scores: opt: 380, E(): 1.5e-18, (45.75% identity in 153 aa overlap); Q9KIF1 FKBR2 from Streptomyces hygroscopicus var. ascomyceticus (175 aa), FASTA scores: opt: 355, E(): 7.6e-17, (42.0% identity in 150 aa overlap); CAC36828|Q99Q03|SAPE Spore associated protein from Streptomyces coelicolor (174 aa), FASTA scores: opt: 318, E(): 2.2e-14, (41.45% identity in 152 aa overlap); etc. I6Y9H2 No intermediary metabolism and respiration O06163 I6Y9H2 GO:0016491,GO:0008152 I6Y9H2 NC_000962.3 Mycobrowser_v5 CDS 2775272 2775565 . + 0 Rv2472 Rv2472 Function unknown Conserved hypothetical protein Rv2472, (MTV008.28), len: 97 aa. Conserved hypothetical protein, showing some similarity to O53451|Rv1103c|MTV017.56c from Mycobacterium tuberculosis strain H37Rv (106 aa), FASTA scores: opt: 135, E(): 0.026, (45.85% identity in 72 aa overlap); and AAK45393|MT1135 hypothetical 11.4 KDA protein from Mycobacterium tuberculosis strain CDC1551 (78 aa) FASTA scores: opt: 139, E(): 0.011, (45.35% identity in 75 aa overlap). O53199 No conserved hypotheticals O53199 NC_000962.3 Mycobrowser_v5 CDS 2782366 2784042 . - 0 Rv2477c Rv2477c Thought to be involved in active transport of macrolide across the membrane (export): macrolide antibiotics resistance by an export mechanism. Responsible for energy coupling to the transport system. Probable macrolide-transport ATP-binding protein ABC transporter Rv2477c, (MTV008.33c), len: 558 aa. Probable ATP binding protein ABC-transporter (see citation below), probably involved in macrolide transport, equivalent to Q9X7B1|MLCB1610.09|ML1248 putative ABC transporter ATP-binding protein from Mycobacterium leprae (556 aa) FASTA scores: opt: 3448, E(): 3.8e-176, (92.3% identity in 557 aa overlap). Also highly similar to many ATP binding proteins e.g. Q9L244|SC6D10.20c putative ABC transporter ATP-binding protein from Streptomyces coelicolor (547 aa), FASTA scores: opt: 2937, E(): 5.6e-149, (79.5% identity in 551 aa overlap); AAK24119|CC2148 ABC transporter ATP-binding protein from Caulobacter crescentus (555 aa), FASTA scores: opt: 2175, E(): 1.9e-108, (59.4% identity in 557 aa overlap); Q9HVJ1 probable ATP-binding component of ABC transporter from Pseudomonas aeruginosa (554 aa), FASTA scores: opt: 2054, E(): 5.1e-102, (56.9% identity in 559 aa overlap); etc. Contains 2 x PS00017 ATP/GTP-binding site motif A (P-loop), 2 x PS00211 ABC transporters family signature, and probable coiled-coil from aa 273 to 311. Belongs to the ATP-binding transport protein family (ABC transporters). P9WQK3 No cell wall and cell processes O53204 P9WQK3 GO:0016887,GO:0005524 P9WQK3 NC_000962.3 Mycobrowser_v5 CDS 2784123 2784608 . - 0 Rv2478c Rv2478c Function unknown Conserved hypothetical protein Rv2478c, (MTV008.34c), len: 161 aa. Conserved hypothetical protein, with weak similarity with many single-strand binding proteins e.g. Q9X8U3|SCH24.29 putative single-strand binding protein from Streptomyces coelicolor (199 aa), FASTA scores: opt: 246, E(): 4.5e-08, (31.5% identity in 162 aa overlap); P46390|SSB_MYCLE|ML2684|MLCB1913.20c single-strand binding protein (SSB) (helix-destabilizing protein) from Mycobacterium leprae (168 aa), FASTA scores: opt: 239, E(): 1e-07, (30.8% identity in 146 aa overlap); P18310|SSBF_ECOLI single-strand binding protein from Escherichia coli (178 aa), FASTA scores: opt: 116, E(): 2.9, (25.7% identity in 140 aa overlap); etc. Also similarity with Rv0054|P71711|MTCY21D4.17|SSB_MYCTU probable single-strand binding protein from M. tuberculosis (164 aa), FASTA scores: opt: 234, E(): 2e-07, (31.75% identity in 148 aa overlap). N-terminus shorter 8 aa from AAK46855|MT2553 single-strand DNA binding protein from Mycobacterium tuberculosis strain CDC1551. O53205 No conserved hypotheticals O53205 O53205 GO:0003697,GO:0006260 O53205 NC_000962.3 Mycobrowser_v5 CDS 2789280 2791022 . - 0 Rv2483c plsC C-terminus: involved in phospholipid biosynthesis (at the second step); converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating acyl moiety at the 2 position [catalytic activity 2: acyl-CoA + 1-acyl-SN-glycerol 3-phosphate = CoA + 1,2-diacyl-SN-glycerol 3-phosphate]. N-terminus: could be generate serine and phosphate from phosphoserine; may catalyze the last step in the biosynthesis of serine from carbohydrates (the reaction mechanism could be proceed via the formation of a phosphoryl-enzyme intermediates) [catalytic activity 1: phosphoserine + H(2)O = serine + phosphate]. Possible transmembrane phospholipid biosynthesis bifunctional enzyme PlsC: putative L-3-phosphoserine phosphatase (O-phosphoserine phosphohydrolase) (PSP) (pspase) + 1-acyl-SN-glycerol-3-phosphate acyltransferase (1-AGP acyltransferase) (1-AGPAT) (lysophosphatidic acid acyltransferase) (LPAAT) Rv2483c, (MTV008.39c), len: 580 aa. Possible plsC, a transmembrane phospholipid biosynthesis bifunctional enzyme, including L-3-phosphoserine phosphatase and 1-acyl-Sn-glycerol-3-phosphate acyltransferase , equivalent to Q9X7A9|PLSC|ML1245 putative acyltransferase from Mycobacterium leprae (579 aa), FASTA scores: opt: 2835, E(): 9.2e-153, (77.15% identity in 573 aa overlap). C-terminal end is similar to many 1-acyl-SN-glycerol-3-phosphate acyltransferases (lysophosphatidic acidacyltransferases) e.g. Q9SDQ2 from Limnanthes floccosa (281 aa), FASTA scores: opt: 378, E(): 3.1e-14, (30.0% identity in 230 aa overlap) and Q42868|PLSC_LIMAL from Limnanthes alba (White meadowfoam) (281 aa), FASTA scores: opt: 374, E(): 5.2e-14, (30.55% identity in 221 aa overlap); and the N-terminal end is similar to many SerB family proteins e.g. AAK44749|MT0526 from Mycobacterium tuberculosis strain CDC1551 (308 aa), FASTA scores: opt: 356, E(): 5.8e-13, (32.5% identity in 298 aa overlap) and Q49823|ML2424 from Mycobacterium leprae (300 aa), FASTA scores: opt: 346, E(): 2.1e-12, (32.0% identity in 278 aa overlap). So belongs to the 1-acyl-SN-glycerol-3-phosphate acyltransferase family and may belong to the SerB family. I6YDI9 No lipid metabolism O53208 I6YDI9 GO:0008152,GO:0016791,GO:0008415 I6YDI9 NC_000962.3 Mycobrowser_v5 CDS 2791019 2792494 . - 0 Rv2484c Rv2484c May be involved in synthesis of triacylglycerol Possible triacylglycerol synthase (diacylglycerol acyltransferase) Rv2484c, (MTV008.40c), len: 491 aa. Possible triacylglycerol synthase (See Daniel et al., 2004), highly similar or similar to many Mycobacterial hypothetical proteins e.g. Q9X7A8|MLCB1610.05|ML1244 conserved membrane protein from Mycobacterium leprae (491 aa), FASTA scores: opt: 2459, E(): 3e-138, (75.15% identity in 483 aa overlap); O53304|YU87_MYCTU|Rv3087|MTV013.08 from Mycobacterium tuberculosis (472 aa), FASTA scores: opt: 527, E(): 8.1e-24, (29.1% identity in 485 aa overlap); O53305|YU88_MYCTU|Rv3088|MT3173|MTV013.09 from Mycobacterium tuberculosis (474 aa), FASTA scores: opt: 370, E(): 1.6e-14, (26.05% identity in 422 aa overlap); etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WKB3 No lipid metabolism O53209 P9WKB3 GO:0006071,GO:0008610,GO:0004144 2.3.1.20 P9WKB3 NC_000962.3 Mycobrowser_v5 CDS 2831344 2832591 . - 0 Rv2515c Rv2515c Function unknown Conserved hypothetical protein Rv2515c, (MTCY07A7.21c), len: 415 aa. Conserved hypothetical protein, showing some similarity to Q9PG06|XF0496 hypothetical protein from Xylella fastidiosa (391 aa), FASTA scores: opt: 388, E(): 4.4e-18, (27.8% identity in 399 aa overlap). Contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. I6XEH5 No conserved hypotheticals O06177 I6XEH5 GO:0043565 I6XEH5 NC_000962.3 Mycobrowser_v5 CDS 2868154 2868567 . + 0 Rv2546 vapC18 Unknown Possible toxin VapC18 Rv2546, (MTCY159.10c), len: 137 aa. Possible vapC18, toxin, part of toxin-antitoxin (TA) operon with Rv2545, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. P96411|Rv0229c|MTCY08D5.24c (226 aa), FASTA scores: opt: 272, E(): 1.3e-11, (39.7% identity in 136 aa overlap); O33299|Rv2757c|MTV002.22c (138 aa), FASTA scores: opt: 265, E(): 2.5e-11, (38.5% identity in 135 aa overlap); P95026|Rv2527|MTCY159.29c (133 aa), FASTA scores: opt: 206, E(): 2.6e-07, (38.0% identity in 100 aa overlap); etc. P95007 No virulence, detoxification, adaptation P95007 P95007 P95007 NC_000962.3 Mycobrowser_v5 CDS 2973795 2975234 . - 0 Rv2650c Rv2650c Unknown Possible PhiRv2 prophage protein Rv2650c, (MTCY441.19), len: 479 aa. Possible phiRv2 prophage protein (capsid subunit) (see citation below), highly similar to O06614|Rv1576c|MTCY336.28 probable phiRv1 phage protein from Mycobacterium tuberculosis (473 aa), FASTA scores: opt: 2782, E(): 2.8e-159, (89.1% identity in 468 aa overlap). P71947 No insertion seqs and phages P71947 NC_000962.3 Mycobrowser_v5 CDS 2794350 2795120 . + 0 Rv2486 echA14 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Probable enoyl-CoA hydratase EchA14 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv2486, (MTV008.42), len: 256 aa. Probable echA14, enoyl-CoA hydratase, similar to others e.g. P24162|ECHH_RHOCA2|FADB1 from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (257 aa), FASTA scores; opt: 453, E(): 3.8e-23, (39.4% identity in 259 aa overlap); Q9ETY7|PACA|PAAG from Azoarcus evansii (273 aa), FASTA scores: opt: 404, E(): 5.7e-17, (37.5% identity in 224 aa overlap); P77467|PAAG_ECOLI from Escherichia coli (262 aa), FASTA scores: opt: 401, E(): 8.3e-17, (36.3% identity in 259 aa overlap); etc. Contains PS00166 Enoyl-CoA hydratase/isomerase signature. Belongs to the enoyl-CoA hydratase/isomerase family. P9WNN5 No lipid metabolism P64018 P9WNN5 GO:0006631,GO:0004300 4.2.1.17 P9WNN5 NC_000962.3 Mycobrowser_v5 CDS 2795301 2797385 . - 0 Rv2487c PE_PGRS42 Function unknown PE-PGRS family protein PE_PGRS42 Rv2487c, (MTV008.43c), len: 694 aa. PE_PGRS42, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of Gly-rich proteins (see citation below), similar to many e.g. AAK47245|MT2919 PE_PGRS family protein from Mycobacterium tuberculosis strain CDC1515 (663 aa), FASTA scores: opt: 2317, E(): 2.3e-84, (58.35% identity in 622 aa overlap); etc. I6XEF1 No PE/PPE Q7D724 I6XEF1 I6XEF1 NC_000962.3 Mycobrowser_v5 CDS 2797467 2800880 . - 0 Rv2488c Rv2488c Involved in transcriptional mechanism. Probable transcriptional regulatory protein (LuxR-family) Rv2488c, (MTV008.44c), len: 1137 aa. Probable transcriptional regulatory protein, belonging to luxR family, similar to many in Mycobacterium tuberculosis e.g. AAK44621|MT0399 from strain CDC1551 (1092 aa) FASTA scores: opt: 3767, E(): 1.8e-211, (56.75% identity in 1093 aa overlap); O53720|Rv0386|MTV036.21 from strain H37Rv (1085 aa), FASTA scores: opt: 3756, E(): 7.6e-211, (56.75% identity in 1089 aa overlap); AAK45665|MT1402 from strain CDC1551 (1159 aa), FASTA scores: opt: 3395, E(): 8.2e-190, (52.0% identity in 1093 aa overlap); etc. Also similar to transcriptional regulatory proteins luxR-family from other organisms e.g. Q9CBP3|ML1753 from Mycobacterium leprae (1106 aa), FASTA scores: opt: 2823, E(): 1.5e-156, (50.35% identity in 1116 aa overlap); Q9KYF4|SCD72A.02 from Streptomyces coelicolor (1114 aa), FASTA scores: opt: 915, E(): 1.7e-45, (30.7% identity in 1143 aa overlap); etc. Some similarity with Q9KXP6|SC9C5.28 hypothetical 81.8 KDA protein from Streptomyces coelicolor (750 aa), FASTA scores: opt: 1085, E(): 1.6e-55, (35.45% identity in 722 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00622 Bacterial regulatory proteins, luxR family signature, probable coiled-coil from aa 585 to 616 and probable helix-turn-helix motif at aa 1086 to 1107 (score 1206, +3.29 SD). Belongs to the LuxR/UhpA family of transcriptional regulators. O53213 No regulatory proteins O53213 O53213 GO:0006915,GO:0009190,GO:0006952,GO:0005622,GO:0016849,GO:0006355,GO:0043565,GO:0003700,GO:0000156,GO:0000160,GO:0005524 O53213 NC_000962.3 Mycobrowser_v5 CDS 2800846 2801145 . - 0 Rv2489c Rv2489c Unknown Hypothetical alanine rich protein Rv2489c, (MTV008.45c), len: 99 aa. Hypothetical unknown ala-rich protein. O53214 No conserved hypotheticals O53214 NC_000962.3 Mycobrowser_v5 CDS 3224708 3226285 . - 0 Rv2916c ffh Necessary for efficient export of extra-cytoplasmic proteins. Binds to the signal sequence when it emerges from the ribosomes. Probable signal recognition particle protein Ffh (fifty-four homolog) (SRP protein) Rv2916c, (MTCY338.04c), len: 525 aa. Probable ffh, signal recognition particle (SRP) protein (ala-, gly-, leu-rich protein) (see citation below), equivalent to O33013|SR54_MYCLE signal recognition particle from Mycobacterium leprae (521 aa), FASTA scores: opt: 2968, E(): 1.6e-145, (87.85% identity in 526 aa overlap). Also highly similar to others e.g. O69874|FFH from Streptomyces coelicolor (550 aa), FASTA scores: opt: 2025, E(): 6e-97, (63.8% identity in 519 aa overlap) (N-terminus longer 34 aa); P37105|SR54_BACSU from Bacillus subtilis (446 aa), FASTA scores: opt: 1451, E(): 1.9e-67, (51.5% identity in 435 aa overlap); BAB57399|FFH from Staphylococcus aureus subsp. aureus Mu50 (455 aa), FASTA scores: opt: 1418, E(): 9.4e-66, (48.65% identity in 448 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the SRP family of GTP-binding proteins. Note that signal recognition particle consists of a small cytoplasmic RNA (SC-RNA) molecule and protein FFH. The protein has a two domain structure: the G-domain binds GTP; the M-domain binds the RNA and also binds the signal sequence. P9WGD7 No cell wall and cell processes P66844 P9WGD7 GO:0005525,GO:0006614,GO:0017111,GO:0005515,GO:0005786,GO:0008312 P9WGD7 NC_000962.3 Mycobrowser_v5 CDS 2808083 2808304 . + 0 Rv2493 vapB38 Unknown Possible antitoxin VapB38 Rv2493, (MTV008.49), len: 73 aa. Possible vapB38, antitoxin, part of toxin-antitoxin (TA) operon with Rv2494, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis strain e.g. Rv3321c|MTV016.21c hypothetical 8.8 KDA protein from Mycobacterium tuberculosis strain H37Rv (80 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WJ25 No virulence, detoxification, adaptation P9WJ25 NC_000962.3 Mycobrowser_v5 CDS 2806665 2807288 . + 0 Rv2491 Rv2491 Function unknown Conserved hypothetical protein Rv2491, (MTV008.47), len: 207 aa. Conserved hypothetical protein, similar in part to other hypothetical proteins e.g. O29139|AF1126 from Archaeoglobus fulgidus (151 aa), FASTA scores: opt: 293, E(): 2.8e-11, (42.85% identity in 126 aa overlap); O66531|AQ_134 from Aquifex aeolicus (151 aa), FASTA scores: opt: 261, E(): 2.6e-09, (37.75% identity in 106 aa overlap); Q9HKU3|TA0501 from Thermoplasma acidophilum (161 aa), FASTA scores: opt: 260, E(): 3.2e-09, (35.9% identity in 117 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6XEF6 No conserved hypotheticals I6XEF6 I6XEF6 NC_000962.3 Mycobrowser_v5 CDS 2807278 2808030 . + 0 Rv2492 Rv2492 Unknown Hypothetical protein Rv2492, (MTV008.48), len: 250 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6YDJ7 No conserved hypotheticals O53217 I6YDJ7 GO:0004799,GO:0006231 NC_000962.3 Mycobrowser_v5 CDS 2808310 2808735 . + 0 Rv2494 vapC38 Unknown Possible toxin VapC38. Contains PIN domain. Rv2494, (MTV008.50), len: 141 aa. Possible vapC38, toxin, part of toxin-antitoxin (TA) operon with Rv2493, contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. P95023|EMBL:Z83863|MTCY159.26|Rv2530c (139 aa) FASTA scores: opt: 380 E(): 6.6e-19, (48.0% identity in 125 aa overlap); O53372|Rv3320c|MTV016.20c (142 aa), etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53219 No virulence, detoxification, adaptation O53219 O53219 NC_000962.3 Mycobrowser_v5 CDS 2827157 2827804 . + 0 Rv2511 orn Involved in RNA degradation: 3'-to-5' exoribonuclease specific for small oligoribonucleotides. Oligoribonuclease Orn Rv2511, (MTCY07A7.17), len: 215 aa. Orn, oligoribonuclease, equivalent to O07708|ORN_MYCLE|ORN|ML0427|MLCL383.34c oligoribonuclease from Mycobacterium leprae (215 aa), FASTA scores: opt: 1170, E(): 3.5e-65, (84.5% identity in 213 aa overlap). Also highly similar to many e.g. P57667|ORN_STRGR|ORNA from Streptomyces griseus (201 aa), FASTA scores: opt: 807, E(): 7.7e-43, (59.0% identity in 200 aa overlap); ORN_STRCO|ORNA|2SC13.01 from Streptomyces coelicolor (200 aa), FASTA scores: opt: 799, E(): 2.4e-42, (59.7% identity in 201 aa overlap); P39287|ORN_ECOLI|B4162 from Escherichia coli strain K12 (180 aa), FASTA scores: opt: 519, E(): 3.9e-25, (47.4% identity in 173 aa overlap); etc. Belongs to the oligoribonuclease family. P9WIU1 No intermediary metabolism and respiration P65597 P9WIU1 GO:0016896,GO:0003676,GO:0005737 3.1.-.- P9WIU1 NC_000962.3 Mycobrowser_v5 CDS 2813727 2814911 . - 0 Rv2500c fadE19 Function unknown, but seems involved in metabolism of small branched-chain fatty acids and macrolide antibiotic production. Catalyses the alpha, beta-dehydrogenation of acyl-CoA esters and transfer electrons to ETF, the electron transfer protein. Possible acyl-CoA dehydrogenase FadE19 (MMGC) Rv2500c, (MTCY07A7.06c), len: 394 aa. Possible fadE19 (alternate gene name: mmgC), acyl-CoA dehydrogenase, similar to many e.g. Q9XCG6|ACDH from Streptomyces coelicolor (386 aa), FASTA scores: opt: 1714, E(): 1.1e-98, (69.45% identity in 383 aa overlap); Q9XCG5|ACDH from Streptomyces avermitilis (386 aa), FASTA scores: opt: 1713, E(): 1.3e-98, (70.0% identity in 383 aa overlap); Q9L7W5|FENK from Bacillus subtilis (370 aa), FASTA scores: opt: 1094, E(): 2.3e-60, (48.4% identity in 372 aa overlap); etc. Contains PS00072 Acyl-CoA dehydrogenases signature 1, PS00073 Acyl-CoA dehydrogenases signature 2. Belongs to the acyl-CoA dehydrogenases family. I6Y0W5 No lipid metabolism O06164 I6Y0W5 GO:0003995,GO:0055114,GO:0050660 1.3.99.- I6Y0W5 NC_000962.3 Mycobrowser_v5 CDS 2809936 2810982 . - 0 Rv2496c bkdB Involved in energy metabolism. The branched-chain alpha-keto acid dehydrogenase complex catalyzes the overall conversion of branched chain alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Probable branched-chain keto acid dehydrogenase E1 component, beta subunit BkdB Rv2496c, (MTCY07A7.02c), len: 348 aa. Probable bkdB, branched-chain keto acid dehydrogenase E1 component, beta subunit, similar to others e.g. Q9Y8I6||PDHB from Halobacterium volcanii (Haloferax volcanii) (327 aa) FASTA scores: opt: 1050, E(): 6.4e-60, (49.7% identity in 324 aa overlap); Q9KG98|BH0214 from Bacillus halodurans (328 aa), FASTA scores: opt: 987, E(): 6.9e-56, (45.7% identity in 324 aa overlap); Q9HN76|PDHB|VNG2218G from Halobacterium sp. strain NRC-1 (297 aa), FASTA scores: opt: 968, E(): 1.1e-54, (51.2% identity in 297 aa overlap); P21874|ODPB_BACST|PDHB pyruvate dehydrogenase E1 component from Bacillus stearothermophilus (324 aa), FASTA scores: opt: 951, E(): 1.4e-53, (47.6% identity in 321 aa overlap); etc. Also similar to Q9XA61|SCGD3.17c putative branched-chain alpha keto acid dehydrogenase E1, beta subunit (2-oxoisovalerate dehydrogenase) from Streptomyces coelicolor, (326 aa), FASTA scores: opt: 1178, E(): 4.1e-68, (55.0% identity in 322 aa overlap); Q9XA48|SCGD3.31c putative branched-chain alpha keto acid dehydrogenase E1 beta subunit from Streptomyces coelicolor (334 aa), FASTA scores: opt: 1173, E(): 8.8e-68, (55.6% identity in 320 aa overlap); Q53593|BKDB E1-beta branched-chain alpha keto acid dehydrogenase from Streptomyces avermitilis (334 aa), FASTA scores: opt: 1132, E(): 3.7e-65, (55.0% identity in 320 aa overlap); etc. Previously known as pdhB. P9WIS1 No intermediary metabolism and respiration O06160 P9WIS1 GO:0055114,GO:0004739,GO:0003863 1.2.4.1,1.2.4.4 P9WIS1 NC_000962.3 Mycobrowser_v5 CDS 2810993 2812096 . - 0 Rv2497c bkdA Involved in energy metabolism. The branched-chain alpha-keto acid dehydrogenase complex catalyzes the overall conversion of branched chain alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Probable branched-chain keto acid dehydrogenase E1 component, alpha subunit BkdA Rv2497c, (MTCY07A7.03c), len: 367 aa. Probable bkdA, branched-chain keto acid dehydrogenase E1 component, alpha subunit, similar to many e.g. Q9Y8I5|PDHA from Halobacterium volcanii (Haloferax volcanii) (368 aa) FASTA scores: opt: 961, E(): 1.3e-52, (45.6% identity in 351 aa overlap); BAB40585 from Bacillus sp. UTB2301 (356 aa) FASTA scores: opt: 947, E(): 9.1e-52, (43.1% identity in 355 aa overlap); Q9KG99|BH0213 from Bacillus halodurans (367 aa), FASTA scores: opt: 896, E(): 1.4e-48, (42.65% identity in 340 aa overlap); etc. Also similar to several putative branched-chain alpha keto acid dehydrogenases E1, beta subunit, alternate name : 2-oxoisovalerate dehydrogenase, e.g. Q53592|BKDA from Streptomyces avermitilis (381 aa), FASTA scores: opt: 980, E(): 8.5e-54, (45.65% identity in 370 aa overlap); etc. Previously known as pdhA. P9WIS3 No intermediary metabolism and respiration O06161 P9WIS3 GO:0006096,GO:0055114,GO:0004739,GO:0030976,GO:0003863 1.2.4.1,1.2.4.4 P9WIS3 NC_000962.3 Mycobrowser_v5 CDS 2812355 2813176 . - 0 Rv2498c citE Interconversion of acetate and oxaloacetate from citrate [catalytic activity: citrate = acetate + oxaloacetate]. Probable citrate (pro-3S)-lyase (beta subunit) CitE (citrase) (citratase) (citritase) (citridesmolase) (citrase aldolase) Rv2498c, (MTCY07A7.04c), len: 273 aa. Probable citE, citrate lyase, beta subunit, similar to others e.g. Q9S3L3|cite from Corynebacterium glutamicum (Brevibacterium flavum) (217 aa), FASTA scores: opt: 565, E(): 1.5e-28, (41.85% identity in 215 aa overlap); Q9HRM8|cite|VNG0627G from Halobacterium sp. strain NRC-1 (303 aa), FASTA scores: opt: 535, E(): 1.5e-26, (41.65% identity in 276 aa overlap); Q9S2U9|SC4G6.02 from Streptomyces coelicolor (274 aa), FASTA scores: opt: 426, E(): 1e-19, (37.6% identity in 274 aa overlap); P77770|CILB_ECOLI from Escherichia coli (307 aa), FASTA scores: opt: 265, E(): 1.5e-10, (32.8% identity in 265 aa overlap); etc. Also similar to Rv3075c|MTCY22D7.06 from Mycobacterium tuberculosis, FASTA score: (35.2% identity in 264 aa overlap). P9WPE1 No intermediary metabolism and respiration 1U5H,1U5V,1Z6K O06162 P9WPE1 GO:0008815,GO:0009346,GO:0046872,GO:0006725 4.1.-.- P9WPE1 NC_000962.3 Mycobrowser_v5 CDS 2822438 2823259 . + 0 Rv2507 Rv2507 Unknown Possible conserved proline rich membrane protein Rv2507, (MTCY07A7.13), len: 273 aa. Possible conserved pro-rich membrane protein (N-terminal half is Proline-rich), highly similar to Q9CCU3|ML0431 putative membrane protein from Mycobacterium leprae (259 aa) (alias O07711|MLCL383.38c but longer 2 aa), FASTA scores: opt: 968, E(): 1.4e-31, (60.35% identity in 275 aa overlap). Contains potential membrane spanning region. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06170 No cell wall and cell processes O06170 O06170 O06170 NC_000962.3 Mycobrowser_v5 CDS 2956893 2957432 . + 0 Rv2630 Rv2630 Unknown Hypothetical protein Rv2630, (MTCY01A10.02c), len: 179 aa. Hypothetical unknown protein. P9WQ03 No conserved hypotheticals O06182 P9WQ03 P9WQ03 NC_000962.3 Mycobrowser_v5 CDS 2818471 2819127 . - 0 Rv2503c scoB Involved in various degradation and synthesis [catalytic activity: succinyl-CoA + a 3-oxo acid = succinate + a 3-oxo-acyl-CoA]. Probable succinyl-CoA:3-ketoacid-coenzyme A transferase (beta subunit) ScoB (3-oxo-acid:CoA transferase) (OXCT B) (succinyl CoA:3-oxoacid CoA-transferase) Rv2503c, (MTCY07A7.09c, MT2578), len: 218 aa. Probable scoB, 3-oxo acid:CoA transferase, beta subunit (succinyl-CoA:3-ketoacid-CoA transferase). Highly similar to others e.g. Q9XAM8|SC4C6.12c from Streptomyces coelicolor (217 aa), FASTA scores: opt: 1048, E(): 2.6e-60, (73.9% identity in 207 aa overlap); Q9XD82|PCAJ from Streptomyces sp. 2065 (214 aa), FASTA scores: opt: 1031, E(): 3.2e-59, (70.8% identity in 209 aa overlap); AAK53493|LPSJ from Xanthomonas campestris (pv. campestris) (212 aa), FASTA scores: opt: 886, E(): 6.6e-50, (62.5% identity in 208 aa overlap); P42316|SCOB_BACSU from Bacillus subtilis (216 aa), FASTA scores: opt: 820, E(): 1.2e-45, (58.2% identity in 201 aa overlap); etc. Belongs to the 3-oxoacid CoA-transferase subunit B family. P9WPW3 No lipid metabolism P63650 P9WPW3 GO:0008152,GO:0008260 2.8.3.5 P9WPW3 NC_000962.3 Mycobrowser_v5 CDS 2819124 2819870 . - 0 Rv2504c scoA Involved in fatty acid degradation/synthesis [catalytic activity: succinyl-CoA + a 3-oxo acid = succinate + a 3-oxo-acyl-CoA]. Probable succinyl-CoA:3-ketoacid-coenzyme A transferase (alpha subunit) ScoA (3-oxo acid:CoA transferase) (OXCT A) (succinyl-CoA:3-oxoacid-coenzyme A transferase) Rv2504c, (MT2579, MTCY07A7.10c), len: 248 aa. Probable scoA, succinyl-CoA:3-ketoacid-Coenzyme A transferase, alpha subunit (3-oxo acid:CoA transferase). Highly similar to others e.g. Q9XAM7|SC4C6.13c from Streptomyces coelicolor (260 aa), FASTA scores: opt: 1130, E(): 2.2e-64, (69.9% identity in 249 aa overlap); Q9XD83|PCAI from Streptomyces sp. 2065 (251 aa), FASTA scores: opt: 1121, E(): 8.1e-64, (69.5% identity in 249 aa overlap); etc. Belongs to the 3-oxoacid CoA-transferase subunit A family. P9WPW5 No lipid metabolism P63648 P9WPW5 GO:0008152,GO:0008260 2.8.3.5 P9WPW5 NC_000962.3 Mycobrowser_v5 CDS 2819953 2821596 . - 0 Rv2505c fadD35 Function unknown, but involved in lipid degradation. Probable fatty-acid-CoA ligase FadD35 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv2505c, (MTCY07A7.11c), len: 547 aa. Probable fadD35, fatty-acid-CoA synthetase, highly similar to many e.g. Q9Z5A6|SC2G5.17 from Streptomyces coelicolor (541 aa), FASTA scores: opt: 2202, E(): 8e-131, (61.55% identity in 528 aa overlap); Q9F9U4|FADD from Pseudomonas stutzeri (Pseudomonas perfectomarina), FASTA scores: opt: 1551, E(): 7.3e-90, (55.55% identity in 551 aa overlap); Q987S7|MLR6932 from Rhizobium loti (Mesorhizobium loti) (590 aa), FASTA scores: opt: 1453, E(): 1.1e-83, (50.7% identity in 564 aa overlap); etc. I6Y0X0 No lipid metabolism O06168 I6Y0X0 GO:0008152,GO:0016874 6.2.1.- I6Y0X0 NC_000962.3 Mycobrowser_v5 CDS 2832710 2833513 . - 0 Rv2516c Rv2516c Unknown Hypothetical protein Rv2516c, (MTV009.01c), len: 267 aa. Hypothetical unknown protein. Contains probable helix-turn-helix motif at aa 98 to 119 (Score 1743, +5.12 SD). C-terminus extended since first submission (+ 18 aa). I6YDM0 No conserved hypotheticals I6YDM0 NC_000962.3 Mycobrowser_v5 CDS 2857853 2858254 . - 0 Rv2532c Rv2532c Unknown Hypothetical protein Rv2532c, (MTCY159.24), len: 133 aa. Hypothetical unknown protein, equivalent to AAK46918 from Mycobacterium tuberculosis strain CDC1551 but shorter 157 aa. P95021 No conserved hypotheticals P95021 P95021 NC_000962.3 Mycobrowser_v5 CDS 3289705 3290235 . + 0 Rv2943A Rv2943A Required for the transposition of an insertion element. Possible transposase Rv2943A, len: 176 aa. Possible transposase, similar to many e.g. AJ238712|MBO238712_2 putative transposase (IS21-l) from Mycobacterium bovis BCG (266 aa), FASTA scores: opt: 762, E(): 0, (100.0% identity in 118 aa overlap). Possible frameshift after codon 118 i.e. near position 3290056, to fuse with Rv2944. Q6MX22 No insertion seqs and phages Q6MX22 Q6MX22 GO:0005524 Q6MX22 NC_000962.3 Mycobrowser_v5 CDS 2825488 2827089 . - 0 Rv2510c Rv2510c Function unknown Conserved protein Rv2510c, (MTCY07A7.16c), len: 533 aa. Conserved protein, highly similar, but longer approximately 20 aa, to others e.g. Q9ABY0|CC0090 hypothetical protein from Caulobacter crescentus (516 aa), FASTA scores: opt: 1282, E(): 8.4e-63, (45.1% identity in 490 aa overlap); Q9A130|SPY0500 hypothetical protein from Streptococcus pyogenes (500 aa), FASTA scores: opt: 1281, E(): 9.3e-63, (43.8% identity in 491 aa overlap); Q985L5|MLR7622 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (515 aa), FASTA scores: opt: 1259, E(): 1.5e-61, (44.1% identity in 510 aa overlap); P39342|YJGR_ECOLI|B4263 hypothetical 54.3 KDA protein from Escherichia coli strain K12 (500 aa), FASTA scores: opt: 1257, E(): 1.9e-61, (42.7% identity in 501 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). I6Y0X6 No conserved hypotheticals O06173 I6Y0X6 I6Y0X6 NC_000962.3 Mycobrowser_v5 CDS 2968533 2968850 . - 0 Rv2644c Rv2644c Unknown Hypothetical protein Rv2644c, (MTCY441.14c), len: 105 aa. Hypothetical unknown protein. P9WL53 No conserved hypotheticals P9WL53 NC_000962.3 Mycobrowser_v5 CDS 2828556 2829803 . - 0 Rv2512c Rv2512c Required for the transposition of the insertion element IS1081. Transposase for insertion sequence element IS1081 Rv2512c, (MTCY07A7.18c), len: 415 aa. Transposase for IS1081, identical to P35882|TRA1_MYCBO transposase for insertion sequence element IS1081 from Mycobacterium bovis (415 aa), FASTA scores: opt: 2680, E(): 1.9e-162, (100.0% identity in 415 aa overlap). Also highly similar to others from Mycobacterium tuberculosis e.g. P96354|Rv1047|MTCY10G2.02c|Rv3115|MTCY164.25|Rv3023c|MTV012.38c (415 aa), FASTA scores: opt: 2675, E(): 3.9e-162, (99.75% identity in 415 aa overlap). Contains PS00435 Peroxidases proximal heme-ligand signature, PS01007 Transposases, Mutator family, signature. Belongs to the mutator family of transposase. P60230 No insertion seqs and phages P60230 P60230 GO:0004803,GO:0006313,GO:0003677 P60230 NC_000962.3 Mycobrowser_v5 CDS 2830161 2830583 . + 0 Rv2513 Rv2513 Unknown Hypothetical protein Rv2513, (MTCY07A7.19), len: 140 aa. Hypothetical unknown protein. O06175 No conserved hypotheticals O06175 NC_000962.3 Mycobrowser_v5 CDS 2849852 2850574 . - 0 Rv2525c Rv2525c Function unknown. Possible role in biosynthesis of the cell wall. Conserved hypothetical protein. Secreted; predicted to be a substrate of the twin arginine translocation (tat) export system. Rv2525c, (MTCY159.31), len: 240 aa. Conserved hypothetical protein, equivalent to Q9X7E1|ML1190|MLCB458.05 hypothetical 25.3 KDA protein from Mycobacterium leprae (239 aa), FASTA scores: opt: 1358, E(): 1e-75, (82.15% identity in 241 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). I6XEI5 No conserved hypotheticals P95028 I6XEI5 I6XEI5 NC_000962.3 Mycobrowser_v5 CDS 2866468 2867127 . + 0 Rv2543 lppA Unknown Probable conserved lipoprotein LppA Rv2543, (MTCY159.13c), len: 219 aa. Probable lppA, conserved lipoprotein, highly similar to upstream ORF P95009|LPPB|Rv2544|MTCY159.12 putative lipoprotein LPPB from Mycobacterium tuberculosis (220 aa), FASTA scores: opt: 1240, E(): 1.1e-73, (87.15% identity in 218 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. P9WK81 No cell wall and cell processes 2V7S P9WK81 GO:0005886 P9WK81 NC_000962.3 Mycobrowser_v5 CDS 2837388 2837615 . - 0 Rv2520c Rv2520c Unknown Possible conserved membrane protein Rv2520c, (MTV009.05c), len: 75 aa. Possible conserved membrane protein, equivalent to O07706|MLCL383.32 hypothetical 10.0 KDA protein from Mycobacterium leprae (91 aa), FASTA scores: opt: 290, E(): 4.1e-14, (58.65% identity in 75 aa overlap); and Q9CCU6|ML0425 putative membrane protein from Mycobacterium leprae (75 aa), FASTA scores: opt: 286, E(): 6.6e-14, (57.35% identity in 75 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). I6XEI0 No cell wall and cell processes O53225 I6XEI0 NC_000962.3 Mycobrowser_v5 CDS 2851091 2851318 . + 0 Rv2526 vapB17 Unknown Possible antitoxin VapB17 Rv2526, (MTCY159.30c), len: 75 aa. Possible vapB17, antitoxin, part of toxin-antitoxin (TA) operon with Rv2527 (See Arcus et al., 2005; Pandey and Gerdes, 2005). P9WJ49 No virulence, detoxification, adaptation P95027 P9WJ49 NC_000962.3 Mycobrowser_v5 CDS 2835785 2837263 . + 0 Rv2519 PE26 Function unknown PE family protein PE26 Rv2519, (MTV009.04), len: 492 aa. PE26, Member of the M. tuberculosis PE family (see citation below), highly similar to many e.g. Q50630|YP91_MYCTU|Rv2591|MT2668.1|MTCY227.10c (543 aa), FASTA scores: opt: 848, E(): 3e-30, (39.55% identity in 445 aa overlap). Q79FD3 No PE/PPE Q79FD3 Q79FD3 GO:0006508,GO:0004190 Q79FD3 NC_000962.3 Mycobrowser_v5 CDS 2837684 2838157 . + 0 Rv2521 bcp Putative antioxidant protein. Probable bacterioferritin comigratory protein Bcp Rv2521, (MTV009.06), len: 157 aa. Probable bcp, bacterioferritin comigratory protein, equivalent to O07705|BCP|ML0424 from Mycobacterium leprae (161 aa), FASTA scores: opt: 829, E(): 6.8e-46, (79.6% identity in 157 aa overlap). Also highly similar to Q9KZQ2|SCE6.38 hypothetical 16.8 KDA protein Streptomyces coelicolor (155 aa), FASTA scores: opt: 727, E(): 2e-39, (69.5% identity in 154 aa overlap); P23480|AAG57590|BCP_ECOLI|B2480|BAB36765|Z3739|ECS3342 bacterioferritin comigratory protein from Escherichia coli strain K12 (156 aa), FASTA scores: opt: 513, E(): 8.3e-26, (48.3% identity in 149 aa overlap); Q9RW23|DR0846 bacterioferritin comigratory protein from Deinococcus radiodurans (175 aa), FASTA scores: opt: 465, E(): 1e-22, (46.5% identity in 157 aa overlap); P44411|BCP_HAEIN|HI0254 bacterioferritin comigratory protein from Haemophilus influenzae (155 aa), FASTA scores: opt: 453, E(): 5.3e-22, (47.5% identity in 139 aa overlap); etc. Also similar to Mycobacterium tuberculosis Rv1608c|MTV046.06|bcpB and Rv2238c|MTCY427.19c|hpE. P9WIE1 No virulence, detoxification, adaptation O53226 P9WIE1 GO:0045454,GO:0016491,GO:0016209 P9WIE1 NC_000962.3 Mycobrowser_v5 CDS 2970123 2970554 . + 0 Rv2645 Rv2645 Unknown Hypothetical protein Rv2645, (MTCY441.15), len: 143 aa. Hypothetical unknown protein. P9WL51 No unknown P9WL51 P9WL51 NC_000962.3 Mycobrowser_v5 CDS 2858254 2858724 . - 0 Rv2533c nusB Involved in the transcription termination process. Interacts with RPSJ|NUSE|Rv0700. N utilization substance protein NusB (NusB protein) Rv2533c, (MT2608, MTCY159.23), len: 156 aa. NusB, N utilization substance protein (see citations below), equivalent to Q9CCR9|NUSB_MYCLE|ML0523 N utilization substance protein B from Mycobacterium leprae (190 aa), FASTA scores: opt: 749, E(): 2.6e-41, (75.7% identity in 148 aa overlap). Also highly similar to others e.g. Q9KXR0|SC9C5.14 from Streptomyces coelicolor (142 aa), FASTA scores: opt: 358, E(): 2.7e-16, (45.0% identity in 140 aa overlap); P54520|NUSB_BACSU from Bacillus subtilis (131 aa), FASTA scores: opt: 315, E(): 1.5e-13, (39.55% identity in 129 aa overlap); O83979|NUSB_TREPA|TP1015 from Treponema pallidum (141 aa), FASTA scores: opt: 268, E(): 1.6e-10, (36.95% identity in 138 aa overlap); etc. Belongs to the NusB family. P9WIV1 No information pathways 1EYV P95020 P9WIV1 GO:0006355,GO:0006353,GO:0003715,GO:0003723 P9WIV1 NC_000962.3 Mycobrowser_v5 CDS 2840123 2849332 . - 0 Rv2524c fas Involved in lipid metabolism. Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. Probable fatty acid synthase Fas (fatty acid synthetase) Rv2524c, (MTCY159.32, MTV009.09c), len: 3069 aa. Probable fas, Fatty Acid Synthase, equivalent to Q9X7E2|fas|ML1191 putative type I fatty acid synthase from Mycobacterium leprae (3076 aa), FASTA scores: opt: 17484, E(): 0, (85.8% identity in 3081 aa overlap). Also similar to others e.g. Q04846|fas|Q59497 from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (3104 aa), FASTA scores: opt: 3981, E(): 5.5e-203, (49.8% identity in 3099 aa overlap); Q48926|fas from Mycobacterium bovis (2796 aa), FASTA scores: opt: 2098, E(): 3.9e-103, (59.7% identity in 2862 aa overlap) (see Fernandes et al., 1996); P34731|FAS1_CANAL fatty acid synthase subunit beta from Candida albicans (Yeast) (2037 aa), FASTA scores: opt: 955, E(): 1.3e-42, (27.4% identity in 1926 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00606 Beta-ketoacyl synthases active site. P95029 No lipid metabolism P95029 P95029 GO:0006633,GO:0004312,GO:0005835,GO:0055114,GO:0016491 2.3.1.- P95029 NC_000962.3 Mycobrowser_v5 CDS 2851315 2851716 . + 0 Rv2527 vapC17 Unknown Possible toxin VapC17 Rv2527, (MTCY159.29c), len: 133 aa. Possible vapC17, toxin, part of toxin-antitoxin (TA) operon with Rv2526, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. P95007|MTCY159.10c|Rv2546 (137 aa), FASTA scores: opt: 206, E(): 1.4e-07, (38.0% identity in 100 aa overlap); O33299|MTV002.22c|Rv2757c (138 aa), FASTA scores: opt: 201, E(): 3.1e-07, (35.7% identity in 126 aa overlap); and P96411|MTCY08D5.24c|Rv0229c (226 aa), FASTA scores: opt: 153, E(): 0.0011, (32.8% identity in 128 aa overlap). P9WF95 No virulence, detoxification, adaptation P95026 P9WF95 P9WF95 NC_000962.3 Mycobrowser_v5 CDS 2852875 2854266 . + 0 Rv2529 Rv2529 Unknown Hypothetical protein Rv2529, (MTCY159.27c), len: 463 aa. Hypothetical unknown protein. Note that C-terminal part is similar to short region of Q53609|MTS1_STRAL|SALIM modification methylase SALI from Streptomyces albus G (587 aa), FASTA scores: opt: 170, E(): 0.016, (59.45% identity in 37 aa overlap). P95024 No conserved hypotheticals P95024 GO:0006259,GO:0004518,GO:0005515,GO:0003677 P95024 NC_000962.3 Mycobrowser_v5 CDS 2854267 2854686 . - 0 Rv2530c vapC39 Unknown Possible toxin VapC39. Contains PIN domain. Rv2530c, (MTCY159.26), len: 139 aa. Possible vapC39, toxin, part of toxin-antitoxin (TA) operon with Rv2530A, contains PIN domain, see Arcus et al. 2005. Highly similar to others in Mycobacterium tuberculosis e.g. O53219|Rv2494|MTV008.50 (141 aa), FASTA scores: opt: 380, E(): 3.6e-19, (48.0% identity in 125 aa overlap); and O53372|Rv3320c|MTV016.20c (142 aa), FASTA scores: opt: 286, E(): 9.3e-13, (41.35% identity in 133 aa overlap); and similar to others e.g. O07760|Rv0617|MTCY19H5.04c (133 aa), FASTA scores: opt: 158, E(): 0.00048, (39.55% identity in 129 aa overlap). Also some similarity with CAC48798|SMB20412 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) plasmid pSymB (54 aa), FASTA scores: opt: 184, E(): 3.7e-06, (53.85% identity in 52 aa overlap); and CAC48797|SMB20411 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) plasmid pSymB (82 aa), FASTA scores: opt: 170, E(): 4.8e-05, (44.45% identity in 63 aa overlap). P9WF63 No virulence, detoxification, adaptation P95023 P9WF63 P9WF63 NC_000962.3 Mycobrowser_v5 CDS 2881409 2881702 . + 0 Rv2561 Rv2561 Function unknown Conserved hypothetical protein Rv2561, (MTCY9C4.07c), len: 97 aa. Conserved hypothetical protein, highly similar in part (and longer 33 aa) to upstream ORF AAK46951|RV2562|MT2638|MTCY9C4.06c conserved hypothetical protein from Mycobacterium tuberculosis (212 aa), FASTA scores: opt: 205, E(): 2e-06, (76.1% identity in 46 aa overlap). Q50737 No conserved hypotheticals Q50737 Q50737 P9WL99 NC_000962.3 Mycobrowser_v5 CDS 2862673 2863203 . - 0 Rv2539c aroK Involved at the fifth step in the biosynthesis of chorismate within the biosynthesis of aromatic amino acids (the shikimate pathway) [catalytic activity: ATP + shikimate = ADP + shikimate 3-phosphate]. Shikimate kinase AroK (SK) Rv2539c, (MTCY159.17), len: 176 aa. AroK, shikimate kinase (see citations below), equivalent to Q9CCS5|AROK|ML0517 putative shikimate kinase from Mycobacterium leprae (199 aa), FASTA scores: opt: 852, E(): 1.3e-42, (79.65% identity in 167 aa overlap). Also highly similar to many e.g. Q9X5D1|AROK_CORG from Corynebacterium glutamicum (Brevibacterium flavum) (169 aa), FASTA scores: opt: 478, E(): 5.4e-21, (47.0% identity in 168 aa overlap); Q9KXQ5|AROK from Streptomyces coelicolor (171 aa), FASTA scores: opt: 465, E(): 3.1e-20, (49.1% identity in 167 aa overlap); P24167|AROK_ECOLI from Escherichia coli strain K12 (172 aa), FASTA scores: opt: 316, E(): 1.3e-11, (38.4% identity in 164 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A, and PS01128 Shikimate kinase signature. Belongs to the shikimate kinase family. P9WPY3 No intermediary metabolism and respiration 1L4U,1L4Y,1U8A,1WE2,1ZYU,2DFN,2DFT,2G1J,2G1K,2IYQ,2IYR,2IYS,2IYT,2IYU,2IYV,2IYW,2IYX,2IYY,2IYZ,3BAF P0A4Z2 P9WPY3 GO:0009073,GO:0005737,GO:0000287,GO:0004765,GO:0005524 2.7.1.71 P9WPY3 NC_000962.3 Mycobrowser_v5 CDS 2864427 2864834 . + 0 Rv2541 Rv2541 Unknown Hypothetical alanine rich protein Rv2541, (MTCY159.15c), len: 135 aa. Hypothetical unknown ala-rich protein, equivalent to AAK46926|MT2615.1 hypothetical 38.9 KDA protein from Mycobacterium tuberculosis strain CDC1551 but AAK46926|MT2615.1 longer at C-terminus. Questionable ORF. Some similarity with Rv2077A from Mycobacterium tuberculosis (99 aa). P95012 No conserved hypotheticals P95012 P95012 NC_000962.3 Mycobrowser_v5 CDS 2863207 2864412 . - 0 Rv2540c aroF Involved at the seventh step in the biosynthesis of chorismate within the biosynthesis of aromatic amino acids (the shikimate pathway) [catalytic activity: 5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + orthophosphate]. Probable chorismate synthase AroF (5-enolpyruvylshikimate-3-phosphate phospholyase) Rv2540c, (MTCY159.16), len: 401 aa. Probable aroF (alternate gene name: aroC), chorismate synthase, equivalent to Q9CCS6|AROF|ML0516 putative chorismate synthase from Mycobacterium leprae (407 aa), FASTA scores: opt: 2278, E(): 6.2e-123, (88.05% identity in 401 aa overlap). Also highly similar to many e.g. Q9X5D0|AROC_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (410 aa), FASTA scores: opt: 1811, E(): 3e-96, (70.3% identity in 397 aa overlap); Q9KXQ4|AROC_STRCO|AROF|SC9C5.20c from Streptomyces coelicolor (394 aa), FASTA scores: opt: 1710, E(): 1.7e-90, (67.0% identity in 385 aa overlap); Q9KCB7|AROC_BACHD|AROF|BH1656 from Bacillus halodurans (390 aa), FASTA scores: opt: 1196, E(): 3.9e-61, (48.7% identity in 386 aa overlap); etc. Contains PS00788 Chorismate synthase signature 2. Belongs to the chorismate synthase family. Cofactor: reduced flavin, NADH P9WPY1 No intermediary metabolism and respiration 1ZTB,2G85,2O11,2O12,2QHF P63611 P9WPY1 GO:0004107,GO:0009073 4.2.3.5 P9WPY1 NC_000962.3 Mycobrowser_v5 CDS 2865130 2866341 . + 0 Rv2542 Rv2542 Function unknown Conserved hypothetical protein Rv2542, (MTCY159.14c), len: 403 aa. Conserved hypothetical protein, highly similar to AAK46927|MT2616 hypothetical 28.0 KDA protein from Mycobacterium tuberculosis strain CDC1551 (265 aa), FASTA scores: opt: 1776, E(): 2.3e-94, (99.25% identity in 265 aa overlap). And similar to several hypothetical proteins from Mycobacterium tuberculosis (strain H37Rv and CDC1551) e.g. P71654|Rv2797c|MTCY16B7.46 (562 aa), FASTA scores: opt: 537, E(): 2.6e-23, (40.75% identity in 292 aa overlap); P71547|Y963_MYCTU|Rv0963c|MT0992|MTCY10D7.11 (266 aa), FASTA scores: opt: 357, E(): 2.6e-13, (34.6% identity in 234 aa overlap); Q10685|YK77_MYCTU|Rv2077c|MT2137|MTCY49.16c (323 aa), FASTA scores: opt: 261, E(): 9.5e-08, (32.7% identity in 211 aa overlap); etc. Also similar to Q9RDQ9|SC4A7.03 putative secreted protein from Streptomyces coelicolor (406 aa), FASTA scores: opt: 247, E(): 7.3e-07, (30.35% identity in 303 aa overlap). P95011 No conserved hypotheticals P95011 P95011 P95011 NC_000962.3 Mycobrowser_v5 CDS 2868606 2868863 . + 0 Rv2547 vapB19 Unknown Possible antitoxin VapB19 Rv2547, (MTCY159.09c), len: 85 aa. Possible vapB19, antitoxin, part of toxin-antitoxin (TA) operon with Rv2548 (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. P71666|YD98_MYCTU|Rv1398c|MT1442|MTCY21B4.15c hypothetical 9.4 KDA protein from (85 aa), FASTA scores: opt: 108, E(): 0.33, (37.1% identity in 62 aa overlap); and to CAC45864|SMC01933 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (71 aa), FASTA scores: opt: 105, E(): 0.46, (28.4% identity in 74 aa overlap); Q97W38|SSO10342 hypothetical protein from Sulfolobus solfataricus (58 aa), FASTA scores: opt: 94, E(): 2.3, (46.95% identity in 49 aa overlap). P95006 No virulence, detoxification, adaptation P95006 P95006 GO:0006355,GO:0003677 NC_000962.3 Mycobrowser_v5 CDS 2870775 2871194 . - 0 Rv2551c Rv2551c Function unknown Conserved hypothetical protein Rv2551c, (MTCY159.05), len: 139 aa. Conserved hypothetical protein, similar to the second part of Q9XAP1|SC10A7.34c putative type IV peptidase from Streptomyces coelicolor (259 aa), FASTA scores: opt: 243, E(): 7.4e-08, (40.95% identity in 144 aa overlap). Also some similarity with other proteins e.g. AAK58497|GSPO GSPO protein from Acetobacter diazotrophicus (261 aa), FASTA scores: opt: 152, E(): 0.025, (33.35% identity in 135 aa overlap). I6Y9M6 No conserved hypotheticals P95002 I6Y9M6 GO:0016020,GO:0004190 NC_000962.3 Mycobrowser_v5 CDS 3107768 3108397 . + 0 Rv2799 Rv2799 Unknown Probable membrane protein Rv2799, (MTCY16B7.44c), len: 209 aa. Probable membrane protein. Predicted to be an outer membrane protein (See Song et al., 2008). I6XFB7 No cell wall and cell processes I6XFB7 NC_000962.3 Mycobrowser_v5 CDS 2869253 2869627 . - 0 Rv2548A Rv2548A Unknown Conserved protein Rv2548A, len: 124 aa. Conserved protein. I6XEK2 No conserved hypotheticals I6XEK2 NC_000962.3 Mycobrowser_v5 CDS 2869727 2870122 . - 0 Rv2549c vapC20 Unknown Possible toxin VapC20 Rv2549c, (MTCY159.07), len: 131 aa. Possible vapC20, toxin, part of toxin-antitoxin (TA) operon with Rv2550c, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Conserved hypothetical protein, showing some similarity to P73415|SLL1715 from Synechocystis sp. strain PCC 6803 (157 aa), FASTA scores: opt: 167, E(): 4.2e-05, (29.45% identity in 129 aa overlap); Q9HHY6|VNG6166H from Halobacterium sp. plasmid pNRC200 strain NRC-1 (144 aa), FASTA scores: opt: 133, E(): 0.011, (29.6% identity in 125 aa overlap); and Q9HSU3|VNG0072H from Halobacterium sp. strain NRC-1 (144 aa), FASTA scores: opt: 113, E(): 0.29, (25.75% identity in 136 aa overlap). P95004 No virulence, detoxification, adaptation P95004 P95004 P95004 NC_000962.3 Mycobrowser_v5 CDS 2870119 2870364 . - 0 Rv2550c vapB20 Unknown Possible antitoxin VapB20 Rv2550c, (MTCY159.06), len: 81 aa. Possible vapB20, antitoxin, part of toxin-antitoxin (TA) operon with Rv2549c (See Arcus et al., 2005; Pandey and Gerdes, 2005). Some similarity to others in M. tuberculosis e.g. Rv0581 P9WJ45 No virulence, detoxification, adaptation P95003 P9WJ45 GO:0006355,GO:0003677 NC_000962.3 Mycobrowser_v5 CDS 2872012 2873265 . - 0 Rv2553c Rv2553c Unknown Probable conserved membrane protein Rv2553c, (MTCY159.03), len: 417 aa. Probable conserved membrane protein, equivalent to Q9CCS8|ML0514 putative membrane protein from Mycobacterium leprae (421 aa), FASTA scores: opt: 1955, E(): 1.1e-111, (72.7% identity in 414 aa overlap). Also similar in part to various proteins e.g. Q9L9G6|NOVB NOVB protein (aminodesoxychorismate lyase) from Streptomyces sphaeroides (284 aa), FASTA scores: opt: 451, E(): 2.9e-2, (37.95% identity in 203 aa overlap); Q9EWY3|2SCG38.36 conserved hypothetical protein from Streptomyces coelicolor (253 aa), FASTA scores: opt: 419, E(): 2.3e-18, (39.2% identity in 171 aa overlap); Q9CHT3|YGCC hypothetical protein from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (550 aa), FASTA scores: opt: 379, E(): 1.2e-15, (23.0% identity in 417 aa overlap); O25309|HP0587 aminodeoxychorismate lyase (PABC) from Helicobacter pylori (Campylobacter pylori) (329 aa), FASTA scores: opt: 290, E(): 2e-10, (31.65% identity in 180 aa overlap); etc. I6XEK6 No cell wall and cell processes P95000 I6XEK6 I6XEK6 NC_000962.3 Mycobrowser_v5 CDS 2873258 2873770 . - 0 Rv2554c Rv2554c Function unknown Conserved protein Rv2554c, (MTCY159.02), len: 170 aa. Conserved protein, equivalent to Q9CCS9|ML0513 hypothetical protein from Mycobacterium leprae (184 aa), FASTA scores: opt: 701, E(): 2e-34, (72.05% identity in 161 aa overlap). Also highly similar to Q9KXQ0|SC9C5.24c hypothetical 17.7 KDA protein from Streptomyces coelicolor (167 aa), FASTA scores: opt: 461, E(): 2.3e-20, (54.65% identity in 150 aa overlap); and similar to other hypothetical proteins e.g. Q9KDE4 from Bacillus halodurans (140 aa), FASTA scores: opt: 291, E(): 1.9e-10, (38.7% identity in 137 aa overlap); P74662|SLL1547 from Synechocystis sp. strain PCC 6803 (152 aa), FASTA scores: opt: 290, (36.55% identity in 145 aa overlap); Q52673|YQGF_RHOCA from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (159 aa), FASTA scores: opt: 246, E(): 8.4e-08, (34.8% identity in 135 aa overlap); etc. P9WGV7 No conserved hypotheticals P67487 P9WGV7 GO:0006281,GO:0005737,GO:0004518,GO:0003676,GO:0006310 3.1.-.- P9WGV7 NC_000962.3 Mycobrowser_v5 CDS 2876576 2876965 . - 0 Rv2556c Rv2556c Function unknown Conserved hypothetical protein Rv2556c, (MTCY09C4.12), len: 129 aa. Conserved hypothetical protein, highly similar to others e.g. Q9EWY5|2SCG38.34 conserved hypothetical protein from Streptomyces coelicolor (140 aa), FASTA scores: opt: 488, E(): 8.2e-26, (58.8% identity in 131 aa overlap); Q9L9G4|NOVD NOVD protein from Streptomyces sphaeroides (143 aa), FASTA scores: opt: 474, E(): 7.2e-25, (60.85% identity in 120 aa overlap); Q9X2I5|TM1872 from Thermotoga maritima (132 aa), FASTA scores: opt: 270, E(): 2.7e-11, (39.55% identity in 129 aa overlap); etc. P9WFP9 No conserved hypotheticals P67121 P9WFP9 P9WFP9 NC_000962.3 Mycobrowser_v5 CDS 2877072 2877746 . + 0 Rv2557 Rv2557 Function unknown; thought to be involved in the persistence in the host. Conserved protein Rv2557, (MTCY9C4.11c), len: 224 aa. Conserved protein, highly similar to upstream ORF Q50740|MTCY9C4.10c|Rv2558|MT2635 conserved hypothetical protein from Mycobacterium tuberculosis (236 aa), FASTA scores: opt: 1007, E(): 6.9e-60, (69.2% identity in 224 aa overlap); and Mb2587 in Mycobacterium bovis (224 aa). P9WLA5 No conserved hypotheticals P9WLA5 P9WLA5 NC_000962.3 Mycobrowser_v5 CDS 2880075 2881052 . + 0 Rv2560 Rv2560 Unknown Probable proline and glycine rich transmembrane protein Rv2560, (MTCY9C4.08c), len: 325 aa. Probable transmembrane protein, pro-, gly-rich protein. P9WLA1 No cell wall and cell processes Q50738 P9WLA1 GO:0005886,GO:0016021 P9WLA1 NC_000962.3 Mycobrowser_v5 CDS 2882290 2883339 . + 0 Rv2563 Rv2563 Thought to be involved in active transport of glutamine across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable glutamine-transport transmembrane protein ABC transporter Rv2563, (MTCY9C4.05c), len: 349 aa. Probable glutamine-transport transmembrane protein ABC transporter (see citation below), highly similar to O53617|Rv0072|MTV030.16 putative ABC-transporter transmembrane subunit from Mycobacterium tuberculosis (349 aa), FASTA scores: opt: 1772, E(): 1.1e-89, (76.2% identity in 349 aa overlap). Also some similarity with various hypothetical proteins e.g. Q9RYN1|DRA0279 hypothetical 37.1 KDA protein from Deinococcus radiodurans (353 aa), FASTA scores: opt: 347, E(): 6.6e-12, (24.35% identity in 357 aa overlap); BAB58522|SAV2360 conserved hypothetical protein from Staphylococcus aureus subsp. aureus Mu50 (351 aa), FASTA scores: opt: 262, E(): 2.9e-07, (19.4% identity in 356 aa overlap); Q9AK94|SC10A9.10c putative ABC transport system transmembrane protein from Streptomyces coelicolor (379 aa), FASTA scores: opt: 172, E(): 0.025, (26.85% identity in 387 aa overlap); etc. P9WG15 No cell wall and cell processes P65007 P9WG15 GO:0005886,GO:0006810,GO:0016021 P9WG15 NC_000962.3 Mycobrowser_v5 CDS 2972435 2973421 . + 0 Rv2649 Rv2649 Required for the transposition of the insertion element IS6110. Probable transposase for insertion sequence element IS6110 Rv2649, (MTCY441.18), len: 328 aa. Probable transposase for IS6110. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2648 and Rv2649, the sequence UUUUAAAG (directly upstream of Rv2649) maybe responsible for such a frameshifting event (see McAdam et al., 1990). P9WKH9 No insertion seqs and phages P0C5G8 P9WKH9 GO:0015074,GO:0006310,GO:0032196,GO:0003677 P9WKH9 NC_000962.3 Mycobrowser_v5 CDS 3021548 3021796 . - 0 Rv2708c Rv2708c Function unknown Conserved hypothetical protein Rv2708c, (MTCY05A6.29), len: 82 aa. Conserved hypothetical protein, equivalent (but shorter 25 aa) to Q49984|ML1016|U1764C hypothetical protein from Mycobacterium leprae (107 aa), FASTA scores: opt: 492, E(): 7.3e-27, (87.8% identity in 82 aa overlap). Also highly similar to Q9L1U7|SCE59.06c hypothetical 10.4 KDA protein from Streptomyces coelicolor (97 aa), FASTA scores: opt: 200, E(): 4.4e-07, (51.6% identity in 62 aa overlap). I6X562 No conserved hypotheticals O07209 I6X562 NC_000962.3 Mycobrowser_v5 CDS 3862947 3863264 . - 0 Rv3445c esxU Unknown ESAT-6 like protein EsxU Rv3445c, (MTCY77.17c), len: 105 aa. EsxU, ESAT-6 like protein (see citations below), showing weak similarity to O30373|VCD|PA2257 pyoverdine biosynthesis protein from Pseudomonas aeruginosa (215 aa), FASTA scores: opt: 103, E(): 5.6, (32.35% identity in 133 aa overlap). Seems to belong to the ESAT6 family. Start changed since first submission (-20 aa). I6Y3I6 No cell wall and cell processes I6Y3I6 NC_000962.3 Mycobrowser_v5 CDS 2893464 2894408 . - 0 Rv2569c Rv2569c Function unknown Conserved hypothetical protein Rv2569c, (MTCY227.32), len: 314 aa. Conserved hypothetical protein, equivalent to Q9CCT2|ML0508 hypothetical protein from Mycobacterium leprae (313 aa), FASTA scores: opt: 1723, E(): 1.9e-95, (84.4% identity in 301 aa overlap); and some similarity with Q49757|YP69_MYCLE|ML0607|MLCL536.03c|B1937_F2_39 hypothetical 31.1 KDA protein from Mycobacterium leprae (279 aa), FASTA scores: opt: 305, E(): 4.5e-11, (33.0% identity in 300 aa overlap). Also similar to to other hypothetical proteins e.g. Q9HUN8|PA4926 from Pseudomonas aeruginosa (311 aa), FASTA scores: opt: 704, E(): 8.7e-35, (39.7% identity in 320 aa overlap); Q98HG8|MLL2875 from Rhizobium loti (Mesorhizobium loti) (294 aa), FASTA scores: opt: 521, E(): 6.5e-24, (35.05% identity in 294 aa overlap); Q9A7W9|CC1600 from Caulobacter crescentus (325 aa), FASTA scores: opt: 510, E(): 3.2e-23, (34.4% identity in 2588 aa overlap); etc. Also some similarity with proteins from Mycobacterium tuberculosis e.g. P71734|Rv2409c|MTCY253.11 conserved hypothetical protein (279 aa), FASTA scores: opt: 312, E(): 1.7e-11, (34.45% identity in 296 aa overlap); and Q50732|Rv2566|MTCY9C4.02 long conserved hypothetical protein (1140 aa), FASTA scores: opt: 252, E(): 2.2e-07, (28.9% identity in 315 aa overlap) P9WL93 No conserved hypotheticals P0A5G5 P9WL93 P9WL93 NC_000962.3 Mycobrowser_v5 CDS 2894512 2894901 . + 0 Rv2570 Rv2570 Function unknown Conserved hypothetical protein Rv2570, (MTCY227.31c), len: 129 aa. Conserved hypothetical protein, similar to Q98GQ7|MLR3218 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (133 aa), FASTA scores: opt: 174, E(): 9.6e-05, (32.25% identity in 124 aa overlap); Q9A390|CC3314 hypothetical protein from Caulobacter crescentus (129 aa), FASTA scores: opt: 155, E(): 0.0017, (33.35% identity in 108 aa overlap); and Q9A2Y0|CC3426 hypothetical protein from Caulobacter crescentus (120 aa), FASTA scores: opt: 144, E(): 0.0083, (32.95% identity in 91 aa overlap). P9WL91 No conserved hypotheticals P65013 P9WL91 NC_000962.3 Mycobrowser_v5 CDS 2898043 2898783 . + 0 Rv2573 Rv2573 Function unknown Conserved hypothetical protein Rv2573, (MTCY227.28c), len: 246 aa. Conserved hypothetical protein, similar to various proteins e.g. Q9ABG6|CC0261 hypothetical protein from Caulobacter crescentus (290 aa), FASTA scores: opt: 516, E(): 5.8e-26, (40.1% identity in 237 aa overlap); Q99R37|SA2393 hypothetical protein (similar to 2-dehydropantoate 2-reductase) from Staphylococcus aureus subsp. aureus N315 (286 aa), FASTA scores: opt: 368, E(): 1.8e-16, (31.75% identity in 230 aa overlap); Q9KPQ9|VC2307 2-dehydropantoate 2-reductase from Vibrio cholerae (296 aa), FASTA scores: opt: 223, E(): 3.9e-07, (27.7% identity in 224 aa overlap); etc. Equivalent to AAK46962 from Mycobacterium tuberculosis strain CDC1551 (275 aa) but shorter 29 aa. P9WIL1 No conserved hypotheticals Q50648 P9WIL1 GO:0050661,GO:0005737,GO:0055114,GO:0015940,GO:0008677 1.1.1.169 P9WIL1 NC_000962.3 Mycobrowser_v5 CDS 2898806 2899309 . + 0 Rv2574 Rv2574 Function unknown Conserved protein Rv2574, (MTCY227.27c), len: 167 aa. Conserved protein, showing similarity with Q9K3N3|SCG20A.07 hypothetical 17.4 KDA protein from Streptomyces coelicolor (157 aa), FASTA scores: opt: 218, E(): 2.8e-08, (30.65% identity in 150 aa overlap). P9WL87 No conserved hypotheticals P65017 P9WL87 P9WL87 NC_000962.3 Mycobrowser_v5 CDS 2921551 2923182 . + 0 Rv2591 PE_PGRS44 Function unknown PE-PGRS family protein PE_PGRS44 Rv2591, (MTCY227.10c), len: 543 aa. PE_PGRS44, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to others e.g. O53845|Rv0834c|MTV043.26c from Mycobacterium tuberculosis (882 aa), FASTA scores: opt: 1813, E(): 5.8e-66, (55.3% identity in 568 aa overlap). Equivalent to AAK46982 from Mycobacterium tuberculosis strain CDC1551 (505 aa) but longer 38 aa. Contains PS00583 pfkB family of carbohydrate kinases signature 1. P9WIE9 No PE/PPE Q50630 P9WIE9 P9WIE9 NC_000962.3 Mycobrowser_v5 CDS 2926986 2927480 . + 0 Rv2598 Rv2598 Function unknown Conserved hypothetical protein Rv2598, (MTCY227.03c), len: 164 aa. Conserved hypothetical protein, showing similarity with hypothetical proteins from Streptomyces coelicolor e.g. Q9X8S3|SCH10.34c (185 aa), FASTA scores: opt: 197, E(): 3.5e-06, (34.75% identity in 167 aa overlap); and Q9L088|SCC24.29c (172 aa), FASTA scores: opt: 149, E(): 0.0053, (37.65% identity in 146 aa overlap). Equivalent to AAK46988 from Mycobacterium tuberculosis strain CDC1551 (154 aa) but longer 10 aa. P9WL71 No conserved hypotheticals P9WL71 NC_000962.3 Mycobrowser_v5 CDS 2982097 2982351 . + 0 Rv2664 Rv2664 Unknown Hypothetical protein Rv2664, (MTCY441.33), len: 84 aa. Hypothetical protein. Some weak similarity to nearby P71964|Rv2667|clpX'|MT2741|MTCY441.36 possible ATP-dependent protease ATP-binding subunit from Mycobacterium tuberculosis (252 aa), FASTA scores: opt: 134, E(): 0.027, (31.15% identity in 77 aa overlap). I6Y9Z5 No conserved hypotheticals I6Y9Z5 NC_000962.3 Mycobrowser_v5 CDS 2927477 2927908 . + 0 Rv2599 Rv2599 Unknown Probable conserved membrane protein Rv2599, (MTCY227.02c), len: 143 aa. Probable conserved membrane protein, equivalent to Q9K536|2599 hypothetical 15.0 KDA protein (fragment) from Mycobacterium paratuberculosis (143 aa), FASTA scores: opt: 691, E(): 1.7e-33, (68.55% identity in 143 aa overlap). Shows weak similarity with Q9L089|SCC24.28c putative lipoprotein from Streptomyces coelicolor (131 aa), FASTA scores: opt: 130, E(): 0.52, (26.45% identity in 136 aa overlap). Contains PS00626 Regulator of chromosome condensation (RCC1) signature 2. Predicted to be an outer membrane protein (See Song et al., 2008). P9WL69 No cell wall and cell processes P9WL69 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2943600 2944985 . - 0 Rv2615c PE_PGRS45 Function unknown PE-PGRS family protein PE_PGRS45 Rv2615c, (MTCY01A10.19), len: 461 aa. PE_PGRS45, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to many e.g. P71664|Rv1396c|MTCY21B4.13c from Mycobacterium tuberculosis (576 aa), FASTA scores: opt: 1629, E(): 4.8e-58, (56.65% identity in 482 aa overlap). Equivalent to AAK47006 from Mycobacterium tuberculosis strain CDC1551 (476 aa) but shorter 15 aa. Q79FC3 No PE/PPE Q79FC3 Q79FC3 Q79FC3 NC_000962.3 Mycobrowser_v5 CDS 2946434 2947111 . + 0 Rv2618 Rv2618 Function unknown Conserved hypothetical protein Rv2618, (MTCY01A10.15c), len: 225 aa. Conserved hypothetical protein, similar in part to Q9EWQ9|SC4C2.03 conserved hypothetical protein from Streptomyces coelicolor (159 aa), FASTA scores: opt: 235, E(): 1.3e-07, (43.7% identity in 103 aa overlap); Q9HLM6|TA0201 hypothetical protein from Thermoplasma acidophilum (215 aa), FASTA scores: opt: 164, E(): 0.0038, (23.4% identity in 201 aa overlap); and to mycobacterial proteins e.g. O06191|Rv2621c|MTCY01A10.11 hypothetical 24.2 KDA protein from Mycobacterium tuberculosis (224 aa), FASTA scores: opt: 149, E(): 0.033, (28.05% identity in 196 aa overlap). O06195 No conserved hypotheticals O06195 O06195 NC_000962.3 Mycobrowser_v5 CDS 3078158 3078985 . - 0 Rv2769c PE27 Function unknown PE family protein PE27 Rv2769c, (MTV002.34c), len: 275 aa. PE27, Member of the Mycobacterium tuberculosis PE family (see citation below), highly similar to many (notably in N-terminal part) e.g. P96361|Rv1040c|MTCY10G2.09 from Mycobacterium tuberculosis (275 aa), FASTA scores: opt: 1111, E(): 5.9e-52, (68.55% identity in 283 aa overlap). Q79FA8 No PE/PPE Q79FA8 Q79FA8 Q79FA8 NC_000962.3 Mycobrowser_v5 CDS 3137009 3137224 . - 0 Rv2830c vapB22 Unknown Possible antitoxin VapB22 Rv2830c, (MTCY16B7.12), len: 71 aa. Possible vapB22, antitoxin, part of toxin-antitoxin (TA) operon with Rv2829c, (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. Z97182|MTCY19H5.26|Rv0596c Hypothetical protein (85 aa), FASTA scores: opt: 88, E(): 1.3, (41.7% identity in 36 aa overlap); and to PHD_BPP1|Q06253 bacteriophage P1 phd gene (73 aa), FASTA scores: opt: 79, E(): 3.8, (35.9% identity in 39 aa overlap). P71622 No virulence, detoxification, adaptation P71622 P71622 P71622 NC_000962.3 Mycobrowser_v5 CDS 2914015 2915736 . - 0 Rv2587c secD Involved in protein export. Part of the prokaryotic protein translocation apparatus which comprise SECA, SECB, SECD, SECE, SECF, SECG and SECY. Probable protein-export membrane protein SecD Rv2587c, (MTCY227.14), len: 573 aa. Probable secD, protein-export membrane protein (integral membrane protein) (see citation below), equivalent to P38387|SECD_MYCLE|ML0487|MLCB1259.05|B1177_C1_164 protein-export membrane protein from Mycobacterium leprae (571 aa), FASTA scores: opt: 2948, E(): 2.6e-97, (80.6% identity in 583 aa overlap). Also similar to others e.g. Q9AE07|SECD from Corynebacterium glutamicum (Brevibacterium flavum) (637 aa), FASTA scores: opt: 1023, E(): 1.9e-29, (44.95% identity in 596 aa overlap); Q53955|SECD_STRCO from Streptomyces coelicolor (570 aa), FASTA scores: opt: 864, E(): 7.2e-24, (38.0% identity in 584 aa overlap); O33517|SECD_RHOCA from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (554 aa), FASTA scores: opt: 551, E(): 7.6e-13, (32.25% identity in 304 aa overlap); etc. Equivalent to AAK46977 from Mycobacterium tuberculosis strain CDC1551 (554 aa) but longer 19 aa. Belongs to the SecD/SecF family, SecD family. Part of the prokaryotic protein translocation apparatus which comprise SECA|Rv3240c, SECD, SECE|Rv0638, SECF|Rv2586c, SECG|Rv1440 and SECY|Rv0732. P9WGP1 No cell wall and cell processes Q50634 P9WGP1 GO:0016021,GO:0006886,GO:0005886,GO:0015628,GO:0055085,GO:0015627,GO:0015450 P9WGP1 NC_000962.3 Mycobrowser_v5 CDS 2925492 2925737 . + 0 Rv2595 vapB40 Unknown Possible antitoxin VapB40 Rv2595, (MTCY227.06c), len: 81 aa. Possible vapB40, antitoxin, part of toxin-antitoxin (TA) operon with Rv2596, see Arcus et al. 2005. Similarity with various bacterial proteins e.g. O28268|AF2011 conserved hypothetical protein from Archaeoglobus fulgidus (86 aa), FASTA scores: opt: 120, E(): 0.13, (34.35% identity in 67 aa overlap); CAC46196|SMC01176 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (79 aa), FASTA scores: opt: 119, E(): 0.14, (33.35% identity in 63 aa overlap); P37554|SP5T_BACSU|SPOVT stage V sporulation protein T from Bacillus subtilis (178 aa), FASTA scores: opt: 104, E(): 2.9, (51.45% identity in 35 aa overlap); etc. Also similar to O07779|Rv0599c|MTCY19H5.23 hypothetical protein from Mycobacterium tuberculosis (78 aa), FASTA scores: opt: 160, E(): 0.00026, (35.8% identity in 81 aa overlap). P9WFC3 No virulence, detoxification, adaptation P65027 P9WFC3 NC_000962.3 Mycobrowser_v5 CDS 2923199 2924233 . - 0 Rv2592c ruvB forms a complex with RUVA. RUVB could possess weak ATPase activity, which will be stimulated by the RUVA protein in the presence of DNA. The RUVA-RUVB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RUVAB is an helicase that mediates the holliday junction migration by localized denaturation and reanneling. Probable holliday junction DNA helicase RuvB Rv2592c, (MTCY227.09), len: 344 aa. Probable ruvB, Holliday junction binding protein (see Mizrahi & Andersen 1998), equivalent to P40833|RUVB_MYCLE|ML0483|B1177_C3_227 holliday junction DNA helicase from Mycobacterium leprae (349 aa), FASTA scores: opt: 2059, E(): 2.1e-106, (94.45% identity in 342 aa overlap). Also highly similar to others e.g. Q9AE09|RUVB from Corynebacterium glutamicum (Brevibacterium flavum) (363 aa), FASTA scores: opt: 1651, E(): 6.5e-84, (75.6% identity in 332 aa overlap); Q9L291|RUVB from Streptomyces coelicolor (357 aa), FASTA scores: opt: 1530, E(): 3e-77, (68.2% identity in 343 aa overlap); P08577|RUVB_ECOLI|B1860|Z2912|ECS2570 from Escherichia coli strains K12 and O157:H7 (336 aa), FASTA scores: opt: 1284, E(): 1e-63, (55.45% identity in 330 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the RuvB family. P9WGW1 No information pathways P66753 P9WGW1 GO:0004176,GO:0003677,GO:0006310,GO:0006281,GO:0009432,GO:0009378,GO:0006508,GO:0004252,GO:0005524 3.6.4.12 P9WGW1 NC_000962.3 Mycobrowser_v5 CDS 2950489 2951307 . - 0 Rv2624c Rv2624c Function unknown Universal stress protein family protein Rv2624c, (MTCY01A10.08), len: 272 aa. Universal stress protein family protein, similar to several Streptomyces proteins e.g. Q9RIY5|SCJ1.29c hypothetical 30.1 KDA protein from Streptomyces coelicolor (283 aa), FASTA scores: opt: 260, E(): 5e-09, (32.05% identity in 290 aa overlap). Also similar to Mycobacterium tuberculosis proteins O53474|Rv2028c|MTV018.15c (279 aa), FASTA scores: opt: 563, E(): 7e-28, (36.85% identity in 266 aa overlap); P95192|Rv3134c|MTCY03A2.240 (268 aa), FASTA scores: opt: 458, E(): 2.3e-21, (36.55% identity in 271 aa overlap); Q10851|YK05_MYCTU|Rv2005c|MT2061|MTCY39.12 (295 aa), FASTA scores: opt: 199, E(): 3.2e-05, (29.35% identity in 286 aa overlap); etc. P9WFD5 No virulence, detoxification, adaptation O06188 P9WFD5 GO:0006950 P9WFD5 NC_000962.3 Mycobrowser_v5 CDS 2930070 2930357 . + 0 Rv2601A vapB41 Unknown Possible antitoxin VapB41 Rv2601A, len: 95 aa. Possible vapB41, antitoxin, part of toxin-antitoxin (TA) operon with Rv2602, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. O53811|Rv0748 conserved hypothetical protein (88 aa), FASTA scores: opt: 132, E(): 0.017, (29.25% identity in 82 aa overlap); O53218|Rv2493 (73 aa), FASTA scores: opt: 107, E(): 0.97, (33.75% identity in 83 aa overlap); and Q10799|YS71_MYCTU|Rv2871 conserved hypothetical protein from Mycobacterium tuberculosis (85 aa), FASTA scores: opt: 108, E(): 0.91, (41.00% identity in 39 aa overlap). P9WJ21 No virulence, detoxification, adaptation P9WJ21 GO:0045449 P9WJ21 NC_000962.3 Mycobrowser_v5 CDS 2943376 2943603 . + 0 Rv2614A Rv2614A Unknown Conserved hypothetical protein Rv2614A, len: 75 aa. Conserved hypothetical protein. The region from aa 10-35 is similar to part of C-terminal part of several triosephosphate isomerases e.g. P46711|TPIS_MYCLE|TPIA|TPI|ML0572|B1496_C1_127 from Mycobacterium leprae (261 aa), FASTA scores: opt: 112, E(): 0.95, (60.0% identity in 25 aa overlap); and O08408|TPIS_MYCTU|TPIA|TPI|Rv1438|MT1482|MTCY493.16c from Mycobacterium tuberculosis (261 aa), FASTA scores: opt: 104, E(): 3.3, (60.0% identity in 25 aa overlap); P19583|TPIS_CORGL|TPIA|TPI from Corynebacterium glutamicum (Brevibacterium flavum) (259 aa), FASTA scores: opt: 100, E(): 6, (45.45% identity in 33 aa overlap); etc. Triosephosphate isomerases play an important role in several metabolic pathways (catalytic activity: D-glyceraldehyde 3-phosphate = dihydroxy-acetone phosphate). Nucleotide position 2943411 in the genome sequence has been corrected, T:C resulting in L12L. Q79FC4 No conserved hypotheticals Q79FC4 Q79FC4 NC_000962.3 Mycobrowser_v5 CDS 2945330 2945830 . + 0 Rv2616 Rv2616 Function unknown Conserved protein Rv2616, (MTCY01A10.18c), len: 166 aa. Conserved protein, highly similar to bacterial proteins: Q9L1G0|SC3D11.02c hypothetical 20.3 KDA protein from Streptomyces coelicolor (188 aa), FASTA scores: opt: 407, E(): 2.3e-20, (44.0% identity in 159 aa overlap); Q9X945 A3(2) glycogen metabolism cluster from Streptomyces coelicolor (134 aa), FASTA scores: opt: 330, E(): 2.5e-15, (46.65% identity in 120 aa overlap) (N-terminus shorter); Q9RST8|DR2035 conserved hypothetical protein from Deinococcus radiodurans (198 aa), FASTA scores: opt: 228, E(): 2.4e-08, (35.1% identity in 168 aa overlap). O06198 No conserved hypotheticals O06198 O06198 NC_000962.3 Mycobrowser_v5 CDS 2932297 2933142 . - 0 Rv2605c tesB2 Function unknown; lipolytic enzyme involved in cellular metabolism. Can hydrolyze a broad range of acyl-CoA thioesters. Probable acyl-CoA thioesterase II TesB2 (TEII) Rv2605c, (MTCY01A10.28), len: 281 aa. Probable tesB2, acyl-CoA thioesterase II, highly similar to others e.g. Q98EG9|MLL4250 from Rhizobium loti (Mesorhizobium loti) (286 aa), FASTA scores: opt: 563, E(): 3.9e-29, (47.75% identity in 287 aa overlap); CAC47767 from Rhizobium meliloti (Sinorhizobium meliloti) (294 aa), FASTA scores: opt: 553, E(): 1.8e-28, (49.3% identity in 280 aa overlap); P23911|TESB_ECOLI|B0452 from Escherichia coli strain K12 (285 aa), FASTA scores: opt: 487, E(): 3.1e-24, (41.9% identity in 277 aa overlap); etc. Also similar to O06135|TESB1|Rv1618|MTCY01B2.10 acyl-CoA thioesterase II from Mycobacterium tuberculosis (300 aa), FASTA scores: opt: 425, E(): 1.1e-21, (34.9% identity in 278 aa overlap). Belongs to the C/M/P thioester hydrolase family. I6X4S7 No lipid metabolism O06209 I6X4S7 GO:0016291,GO:0006637 3.1.2.- I6X4S7 NC_000962.3 Mycobrowser_v5 CDS 2936810 2937865 . - 0 Rv2609c Rv2609c Unknown Probable conserved membrane protein Rv2609c, (MTCY01A10.24), len: 351 aa. Probable conserved membrane protein, equivalent to O07146|MLCL581.13c|ML0451 hypothetical 37.9 KDA protein from Mycobacterium leprae (349 aa), FASTA scores: opt: 1675, E(): 1.4e-95, (77.85% identity in 334 aa overlap). Also similar to hypothetical proteins: O69888|SC2E1.17|mutt hypothetical 19.4 KDA protein from Streptomyces coelicolor and Streptomyces lividans (172 aa), FASTA scores: opt: 345, E(): 3.5e-14, (44.7% identity in 161 aa overlap); Q9L285|SCL2.14c hypothetical 19.8 KDA protein from Streptomyces coelicolor (180 aa), FASTA scores: opt: 179, E(): 0.00056, (43.25% identity in 171 aa overlap); and Q9RYE5|DR0004 mutt/NUDIX family protein from Deinococcus radiodurans (350 aa), FASTA scores: opt: 153, E(): 0.037, (33.35% identity in 123 aa overlap). Contains PS00893 mutT domain signature. Belongs to the mutt/NUDIX family. I6YDV4 No cell wall and cell processes O06205 I6YDV4 GO:0016787 I6YDV4 NC_000962.3 Mycobrowser_v5 CDS 2971659 2972027 . + 0 Rv2647 Rv2647 Unknown Hypothetical protein Rv2647, (MTCY441.17), len: 122 aa (questionable ORF). Hypothetical protein, probably corresponds to conserved DNA sequence also found in MTCY336.29c and Rv1574|MTCY336.30c|O06616 hypothetical 11.4 KDA protein from Mycobacterium tuberculosis (103 aa), FASTA scores: opt: 170, E(): 0.0002, (69.05% identity in 42 aa overlap). Shows weak similarity with Q9EUM1|RESB resolvase protein homolog from Corynebacterium glutamicum (Brevibacterium flavum) (343 aa), FASTA scores: opt: 112, E(): 2.9, (31.05% identity in 87 aa overlap). I6YDZ2 No insertion seqs and phages I6YDZ2 NC_000962.3 Mycobrowser_v5 CDS 2947884 2948558 . - 0 Rv2621c Rv2621c Could be involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv2621c, (MTCY01A10.11), len: 224 aa. Possible transcriptional regulator, similar in part to Q49688|MLCL536.29c|ML0592 putative DNA-binding protein from Mycobacterium leprae (254 aa), FASTA scores: opt: 168, E(): 0.0018, (29.75% identity in 222 aa overlap). Shows similarity with Q9XAD0|SCC22.08c putative DNA-binding protein from Streptomyces coelicolor (252 aa), FASTA scores: opt: 148, E(): 0.032, (29.4% identity in 204 aa overlap); and Q9RVM8|DR0999 conserved hypothetical protein from Deinococcus radiodurans (225 aa), FASTA scores: opt: 195, E(): 3.3e-05, (29.6% identity in 213 aa overlap). Also some similarity with O06195|Rv2618|MTCY01A10.15c from Mycobacterium tuberculosis (225 aa), FASTA scores: opt: 149, E(): 0.025, (28.95% identity in 197 aa overlap). Contains helix-turn-helix motif at aa 31-52 (Score 1662, +4.85 SD). I6Y187 No regulatory proteins O06191 I6Y187 GO:0006355,GO:0003677 I6Y187 NC_000962.3 Mycobrowser_v5 CDS 2951322 2952503 . - 0 Rv2625c Rv2625c Unknown Probable conserved transmembrane alanine and leucine rich protein Rv2625c, (MTCY01A10.07), len: 393 aa. Probable conserved transmembrane ala-, leu-rich protein, similar to many hypothetical or membrane proteins e.g. Q55518|Y528_SYNY3|SLL0528 potential integral membrane protein from Synechocystis sp. strain PCC 6803 (379 aa), FASTA scores: opt: 552, E(): 5.6e-26, (30.75% identity in 374 aa overlap); Q9RJ56|SCI41.35c hypothetical 39.8 KDA protein from Streptomyces coelicolor (374 aa), FASTA scores: opt: 419, E(): 5.7e-18, (31.6% identity in 383 aa overlap); CAC49448|SMB20925 conserved hypothetical membrane protein from Rhizobium meliloti (Sinorhizobium meliloti) (372 aa), FASTA scores: opt: 401, E(): 6.9e-17, (29.5% identity in 383 aa overlap); etc. Contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. P9WHR1 No cell wall and cell processes O06187 P9WHR1 GO:0046872,GO:0004222,GO:0005886,GO:0006508,GO:0016021 P9WHR1 NC_000962.3 Mycobrowser_v5 CDS 2979049 2979309 . - 0 Rv2657c Rv2657c Unknown Probable PhiRv2 prophage protein Rv2657c, (MTCY441.26c), len: 86 aa. Probable phiRv2 phage protein (excisionase) (see citation below), similar to O22001|VG36_BPMD2|36|G2 gene 36 protein (GP36) from Mycobacteriophage D29 (56 aa), FASTA scores: opt: 171, E(): 9.6e-06, (48.0% identity in 50 aa overlap); and Q05246|VG36_BPML5|36 gene 36 protein (GP36) from Mycobacteriophage L5 (56 aa), FASTA scores: opt: 169, E(): 1.3e-05, (50% identity in 50 aa overlap). Similarity suggests alternative start at 21737. Contains possible helix-turn-helix motif from aa 33 to 54 (Score 1655, +4.82 SD). I6YE30 No insertion seqs and phages I6YE30 GO:0003677 I6YE30 NC_000962.3 Mycobrowser_v5 CDS 3100202 3101581 . - 0 Rv2791c Rv2791c Required for the transposition of the insertion element IS1602. Probable transposase Rv2791c, (MTV002.56c), len: 459 aa. Probable IS1602 transposase for IS1602 element, similar to many e.g. P95117|Rv2978c|MTCY349.09 from Mycobacterium tuberculosis (459 aa), FASTA scores: opt: 2718, E(): 6.3e-165, (86.05% identity in 459 aa overlap). O33333 No insertion seqs and phages O33333 O33333 O33333 NC_000962.3 Mycobrowser_v5 CDS 2952562 2952993 . - 0 Rv2626c hrp1 Function unknown Hypoxic response protein 1 Hrp1 Rv2626c, (MTCY01A10.06), len: 143 aa. Hrp1, hypoxic response protein 1, similar to CAC49670|SMB21441 putative inosine-5'-monophosphate dehydrogenase protein from Rhizobium meliloti (Sinorhizobium meliloti) (120 aa), FASTA scores: opt: 287, E(): 6.6e-12, (43.75% identity in 112 aa overlap) (has its N-terminus shorter 27 aa); AAK78655|CAC0678 CBS domains from Clostridium acetobutylicum (142 aa), FASTA scores: opt: 276, E(): 3.9e-11, (35.65% identity in 115 aa overlap); Q9K9P0|BH2605 BH2605 protein from Bacillus halodurans (142 aa), FASTA scores: opt: 276, E(): 3.9e-11, (35.65% identity in 115 aa overlap); etc. Also some similarity to P71737|Rv2406c|MTCY253.14 hypothetical 15.1 KDA protein from Mycobacterium tuberculosis (142 aa), FASTA scores: opt: 145, E(): 0.00012, (22.3% identity in 112 aa overlap). Predicted possible vaccine candidate (See Zvi et al., 2008). P9WJA3 No conserved hypotheticals 1XKF,1Y5H O06186 P9WJA3 GO:0046872,GO:0005576 P9WJA3 NC_000962.3 Mycobrowser_v5 CDS 2953507 2954748 . - 0 Rv2627c Rv2627c Function unknown Conserved protein Rv2627c, (MTCY01A10.05), len: 413 aa. Conserved protein. Some similarity in C-terminal part of O53697|Rv0293c|MTV035.21c hypothetical 44.0 KDA protein from Mycobacterium tuberculosis (400 aa), FASTA scores: opt: 392, E(): 1.9e-17, (31.1% identity in 299 aa overlap). Alternative nucleotide at position 2954439 (T->C; R104G) has been observed. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WL67 No conserved hypotheticals O06185 P9WL67 P9WL67 NC_000962.3 Mycobrowser_v5 CDS 2955058 2955420 . + 0 Rv2628 Rv2628 Unknown Hypothetical protein Rv2628, (MTCY01A10.04c), len: 120 aa. Hypothetical unknown protein. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WL65 No conserved hypotheticals P9WL65 NC_000962.3 Mycobrowser_v5 CDS 2955767 2956891 . + 0 Rv2629 Rv2629 Function unknown Conserved protein Rv2629, (MTCY01A10.03c), len: 374 aa. Conserved protein, similar to Q9ZC00|SC1E6.22c hypothetical 40.7 KDA protein from Streptomyces coelicolor (373 aa), FASTA scores: opt: 425, E(): 2.5e-18, (30.2% identity in 371 aa overlap). Predicted possible vaccine candidate (See Zvi et al., 2008). P9WL63 No conserved hypotheticals P9WL63 P9WL63 NC_000962.3 Mycobrowser_v5 CDS 2965939 2966397 . + 0 Rv2641 cadI Function unknown Cadmium inducible protein CadI Rv2641, (MTCY441.11), len: 152 aa. CadI, conserved hypothetical protein. Gene induced by cadmium (see Hotter et al., 2001), highly similar to hypothetical proteins e.g. Q9L222|SC1A2.19c from Streptomyces coelicolor (152 aa), FASTA scores: opt: 509, E(): 2.3e-27, (55.05% identity in 149 aa overlap); P45945|YQCK_BACSU from Bacillus subtilis (146 aa), FASTA scores: opt: 295, E(): 5.4e-13, (33.55% identity in 146 aa overlap); and Q98CF8|MLL5167 from Rhizobium loti (Mesorhizobium loti) (124 aa), FASTA scores: opt: 110, E(): 1.3, (31.4% identity in 121 aa overlap). Some similarity with Q10548|Y887_MYCTU|Rv0887c|MT0910|MTCY31.15c from Mycobacterium tuberculosis (152 aa), FASTA scores: opt: 108, E(): 2.1, (25.7% identity in 148 aa overlap). P9WIR5 No conserved hypotheticals P0A5N6 P9WIR5 P9WIR5 NC_000962.3 Mycobrowser_v5 CDS 2960105 2962441 . - 0 Rv2634c PE_PGRS46 Function unknown PE-PGRS family protein PE_PGRS46 Rv2634c, (MTCY441.04c), len: 778 aa. PE_PGRS46, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to many e.g. O53553|YZ08_MYCTU|Rv3508|MTV023.15 from Mycobacterium tuberculosis (1901 aa), FASTA scores: opt: 2553, E(): 2.2e-93, (53.8% identity in 866 aa overlap). Equivalent to AAK47026 from Mycobacterium tuberculosis strain CDC1551 (788 aa) but shorter 10 aa. P9WIE7 No PE/PPE P0A690 P9WIE7 P9WIE7 NC_000962.3 Mycobrowser_v5 CDS 2962713 2963390 . + 0 Rv2636 Rv2636 Function unknown Conserved hypothetical protein Rv2636, (MTCY441.06), len: 225 aa. Conserved hypothetical protein, showing some similarity with various proteins: Q98FG2|MLL3789 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (239 aa), FASTA scores: opt: 304, E(): 3.7e-13, (31.55% identity in 187 aa overlap); CAC46568|SMC04451 putative chloramphenicol phosphotransferase protein from Rhizobium meliloti (Sinorhizobium meliloti) (220 aa), FASTA scores: opt: 175, E(): 0.00014, (28.0% identity in 225 aa overlap); Q56148|CPT_STRVL chloramphenicol 3-O phosphotransferase from Streptomyces violaceus (Streptomyces venezuelae) (178 aa), FASTA scores: opt: 131, E(): 0.1, (31.75% identity in 170 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Translational start site uncertain, chosen by similarity. P9WL55 No conserved hypotheticals P65039 P9WL55 GO:0016740,GO:0005524 2.7.1.- P9WL55 NC_000962.3 Mycobrowser_v5 CDS 2964405 2964851 . + 0 Rv2638 Rv2638 Function unknown Conserved hypothetical protein Rv2638, (MTCY441.08), len: 148 aa. Conserved hypothetical protein, similar in part to Q9WVX8|RSBV_STRCO|bldg|SCH5.12c anti-sigma B factor antagonist from Streptomyces coelicolor (113 aa), FASTA scores: opt: 162, E(): 0.00066, (31.8% identity in 110 aa overlap); and showing weak similarity with various proteins e.g. O69205 hypothetical 13.4 KDA protein from Actinosynnema pretiosum (subsp. auranticum) (128 aa), FASTA scores: opt: 157, E(): 0.0016, (29.8% identity in 114 aa overlap); Q9RJ93|SCF91.32 putative anti-sigma factor antagonist from Streptomyces coelicolor (183 aa), FASTA scores: opt: 148, E(): 0.0082, (30.85% identity in 107 aa overlap); etc. Also highly similar to hypothetical proteins from Mycobacterium tuberculosis: O07728|Rv1904|MTCY180.14c (143 aa), FASTA scores: opt: 456, E(): 3.9e-23, (52.8% identity in 125 aa overlap); and Q11035|YD65_MYCTU|Rv1365c|MT1411|MTCY02B10.29c (128 aa), FASTA scores: opt: 435, E(): 8.6e-22, (53.6% identity in 125 aa overlap). I6X4W0 No conserved hypotheticals P71937 I6X4W0 GO:0006355,GO:0045152 I6X4W0 NC_000962.3 Mycobrowser_v5 CDS 2981482 2981754 . + 0 Rv2662 Rv2662 Unknown Hypothetical protein Rv2662, (MTCY441.31), len: 90 aa. Hypothetical unknown protein. P71959 No conserved hypotheticals P71959 P71959 NC_000962.3 Mycobrowser_v5 CDS 2981853 2982086 . + 0 Rv2663 Rv2663 Unknown Hypothetical protein Rv2663, (MTCY441.32), len: 77 aa. Hypothetical unknown protein. I6X520 No conserved hypotheticals I6X520 NC_000962.3 Mycobrowser_v5 tRNA 2969753 2969825 . + 0 MTB000036 glyT - tRNA-Gly (GCC) glyT, tRNA-Gly, anticodon gcc, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 2969855 2969925 . + 0 MTB000037 cysU - tRNA-Cys (GCA) cysU, tRNA-Cys, anticodon gca, length = 71 No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 2969942 2970013 . + 0 MTB000038 valU - tRNA-Val (GAC) valU, tRNA-Val, anticodon gac, length = 72 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 2966533 2966913 . + 0 Rv2642 Rv2642 Involved in transcriptional mechanism. Possible transcriptional regulatory protein (probably ArsR-family) Rv2642, (MTCY441.12), len: 126 aa. Possible transcriptional regulator, arsR family, highly similar to many e.g. Q9X8X8|SCH35.28c putative transcriptional regulator from Streptomyces coelicolor (122 aa), FASTA scores: opt: 390, E(): 3.7e-19, (56.55% identity in 122 aa overlap); Q9L220|SC1A2.21 putative ArsR-family transcriptional from Streptomyces coelicolor (119 aa), FASTA scores: opt: 378, E(): 2.3e-18, (59.8% identity in 97 aa overlap); Q9L1V5|SC4A9.07 putative ArsR-family transcriptional regulator from Streptomyces coelicolor (117 aa), FASTA scores: opt: 359, E(): 4.1e-17, (56.9% identity in 116 aa overlap); P52144|ARR2_ECOLI|ARSR from Escherichia coli (117 aa), FASTA scores: opt: 202, E(): 1e-06, (39.8% identity in 88 aa overlap); etc. Also similar to downstream ORF P71939|Rv2640c|MTCY441.10c putative transcriptional regulatory protein from Mycobacterium tuberculosis (119 aa), FASTA scores: opt: 237, E(): 5e-09, (38.55% identity in 109 aa overlap); and others from Mycobacterium tuberculosis e.g. O05840|Rv2358|MTCY27.22c. Contains PS00846 Bacterial regulatory proteins, arsR family signature. Contains helix-turn-helix motif at aa 58-79 (Score 1112, +2.97 SD). Belongs to the ArsR family of transcriptional regulators. P71941 No regulatory proteins P71941 P71941 GO:0006355,GO:0006350,GO:0003700,GO:0005622 P71941 NC_000962.3 Mycobrowser_v5 CDS 2978660 2979052 . - 0 Rv2656c Rv2656c Unknown Possible PhiRv2 prophage protein Rv2656c, (MTCY441.25c), len: 130 aa. Probable phiRv2 phage protein (see Hatfull 2000), highly similar to O06607|YF83_MYCTU|Rv1583c|MT3573.2|MTCY336.21 Probable phiRV1 phage protein from Mycobacterium tuberculosis (132 aa), FASTA scores: opt: 734, E(): 2.5e-39, (81.5% identity in 131 aa overlap); and some similarity with Q982T4|MLL8506 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (204 aa), FASTA scores: opt: 104, E(): 9.7, (31.85% identity in 113 aa overlap). P9WL49 No insertion seqs and phages P9WL49 NC_000962.3 Mycobrowser_v5 CDS 2970551 2971549 . + 0 Rv2646 Rv2646 Sequence integration. Integrase is necessary for integration of a phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome. Probable integrase Rv2646, (MTCY441.16), len: 332 aa. Probable integrase, similar to others e.g. P06723|VINT_BP186|int integrase from Bacteriophage 186 (336 aa)s FASTA scores: opt: 198, E(): 6.3e-05, (30.45% identity in 138 aa overlap). Could be belong to the 'phage' integrase family. I6XEU5 No insertion seqs and phages P71945 I6XEU5 GO:0015074,GO:0006310,GO:0003677 I6XEU5 NC_000962.3 Mycobrowser_v5 CDS 2972160 2972486 . + 0 Rv2648 Rv2648 Required for the transposition of the insertion element IS6110. Probable transposase for insertion sequence element IS6110 (fragment) Rv2648, (MTCY441.17A), len: 108 aa. Putative Transposase for IS6110 (fragment). Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2648 and Rv2649, the sequence UUUUAAAG (directly upstream of Rv2649) maybe responsible for such a frameshifting event (see McAdam et al., 1990). P9WKH5 No insertion seqs and phages P0C5G9 P9WKH5 GO:0004803,GO:0006313,GO:0003677 P9WKH5 NC_000962.3 Mycobrowser_v5 CDS 2975242 2975775 . - 0 Rv2651c Rv2651c Function unknown; possibly hydrolyzes peptides and/or proteins. Possible PhiRv2 prophage protease Rv2651c, (MTCY441.20c), len: 177 aa. Possible protease protein, phiRv2 phage protein (prohead protease) (see citation below), showing some similarity with several proteases e.g. Q9A4P4|CC2786 putative protease from Caulobacter crescentus (138 aa), FASTA scores: opt: 206, E(): 2e-06, (36.35% identity in 132 aa overlap); Q9RNH0 putative prohead protease from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (184 aa), FASTA scores: opt: 196, E(): 1.1e-05, (35.05% identity in 137 aa overlap); BAB35014|ECS1591 putative prohead protease from Escherichia coli strain O157:H7 (185 aa), FASTA scores: opt: 187, E(): 4.1e-05, (32.9% identity in 158 aa overlap); etc. And highly similar to O06613|Rv1577c|MTCY336.27 Probable phiRV1 phage protein from Mycobacterium tuberculosis (170 aa), FASTA scores: opt: 987, E(): 2.3e-56, (89.35% identity in 169 aa overlap). I6XEX3 No insertion seqs and phages P71948 I6XEX3 GO:0008233 3.4.-.- NC_000962.3 Mycobrowser_v5 CDS 2975928 2976554 . - 0 Rv2652c Rv2652c Unknown Probable PhiRv2 prophage protein Rv2652c, (MTCY441.21c), len: 208 aa. Probable phiRv2 phage protein (terminase) (see citation below), showing some similarity with AAK79859|Q97HW1|CAC1896 phage terminase-like protein (small subunit) from Clostridium acetobutylicum (151 aa), FASTA scores: opt: 155, E(): 0.012, (24.7% identity in 158 aa overlap); and Q9B019 hypothetical 17.8 KDA protein from Bacteriophage GMSE-1 (159 aa), FASTA scores: opt: 141, E(): 0.087, (27.65% identity in 159 aa overlap). Also highly similar to O06612|Rv1578c|MTCY336.26 Probable phiRV1 phage protein from Mycobacterium tuberculosis (156 aa), FASTA scores: opt: 448, E(): 1.2e-20, (48.1% identity in 156 aa overlap). Equivalent to AAK47043 from Mycobacterium tuberculosis strain CDC1551 but longer 45 aa. P71949 No insertion seqs and phages P71949 NC_000962.3 Mycobrowser_v5 CDS 2982699 2982980 . + 0 Rv2665 Rv2665 Function unknown Hypothetical arginine rich protein Rv2665, (MTCY441.34), len: 93 aa. Hypothetical arg-rich protein, showing some similarity to N-terminus of P71640|Rv2811|MTCY16B7.32c hypothetical 21.1 KDA protein from Mycobacterium tuberculosis (202 aa), FASTA scores: opt: 157, E(): 0.0011, (37.5% identity in 72 aa overlap); and also to part of O35132|CP2B_RAT|CYP27B1|CYP27B 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial precursor from Rattus norvegicus (Rat) (501 aa), FASTA scores: opt: 106, E(): 5.4, (34.5% identity in 87 aa overlap). I6Y1F9 No conserved hypotheticals I6Y1F9 NC_000962.3 Mycobrowser_v5 CDS 2980963 2981190 . - 0 Rv2660c Rv2660c Unknown Hypothetical protein Rv2660c, (MTCY441.29c), len: 75 aa (questionable orf). Hypothetical unknown protein. I6Y1F5 No conserved hypotheticals I6Y1F5 NC_000962.3 Mycobrowser_v5 CDS 2981187 2981576 . - 0 Rv2661c Rv2661c Unknown Hypothetical protein Rv2661c, (MTCY441.30c), len: 129 aa. Hypothetical unknown protein. P71958 No conserved hypotheticals P71958 NC_000962.3 Mycobrowser_v5 CDS 2983896 2984654 . + 0 Rv2667 clpC2 Function unknown; possibly hydrolyzes peptides and/or proteins in presence of ATP. Possible ATP-dependent protease ATP-binding subunit ClpC2 Rv2667, (MTCY441.36), len: 252 aa. Possible clpC2, ATP-dependent protease atp-binding subunit, highly similar to Q9X8L2|SCE9.40 hypothetical 27.3 KDA protein from Streptomyces coelicolor (258 aa), FASTA scores: opt: 877, E(): 2.2e-46, (57.25% identity in 255 aa overlap). The second half of the protein is highly similar to N-terminal of several CLP-family proteins e.g. P24428|CLPC_MYCLE|ML0235 probable ATP-dependent CLP protease ATP-binding subunit from Mycobacterium leprae (848 aa), FASTA scores: opt: 307, E(): 3.2e-11, (38.6% identity in 158 aa overlap); O06286|CLPC_MYCTU|Rv3596c|MT3703|MTCY07H7B.26 probable ATP-dependent CLP protease ATP-binding subunit from Mycobacterium tuberculosis (848 aa), FASTA scores: opt: 307, E(): 3.2e-11, (38.6% identity in 158 aa overlap); Q9S6T8|SCE94.24c putative CLP-family ATP-binding protease from Streptomyces coelicolor (841 aa), FASTA scores: opt: 303, E(): 5.6e-11, (38.8% identity in 152 aa overlap); etc. Some weak similarity to nearby P71961|MTCY441.33|Rv2664 hypothetical protein from Mycobacterium tuberculosis (83 aa). Contain Pfam match to entry PF02861 Clp amino terminal domain. Belongs to the CLPA/CLPB family. CLPC subfamily. Note that previously known as clpX' P9WPC7 No intermediary metabolism and respiration P0A524 P9WPC7 GO:0019538,GO:0005515 P9WPC7 NC_000962.3 Mycobrowser_v5 CDS 2984733 2985254 . + 0 Rv2668 Rv2668 Unknown Possible exported alanine and valine rich protein Rv2668, (MTCY441.37), len: 173 aa. Hypothetical ala-, val-rich protein, possibly exported. Equivalent to AAK47057 from Mycobacterium tuberculosis strain CDC1551 (208 aa) but N-terminal part shorter 35 aa and with few differences. Has potential signal peptide sequence. Predicted to be an outer membrane protein (See Song et al., 2008). P71965 No cell wall and cell processes P71965 P71965 NC_000962.3 Mycobrowser_v5 CDS 2990706 2991116 . + 0 Rv2674 msrB Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine [catalytic activity: protein L-methionine + oxidized thioredoxin + H2O = protein-L-methionine-(R)-S-oxide + reduced thioredoxin] Probable peptide methionine sulfoxide reductase MsrB (protein-methionine-R-oxide reductase) (peptide met(O) reductase) Rv2674, (MTCY441.43), len: 136 aa. Probable msrB, peptide methionine sulfoxide reductase (See Lee et al., 2008), highly similar to various proteins e.g. Q9X828|SC9B1.08 putative oxidoreductase from Streptomyces coelicolor (135 aa), FASTA scores: opt: 653, E(): 1.8e-37, (71.1% identity in 128 aa overlap); O26807|MTH711 transcriptional regulator from Methanothermobacter thermautotrophicus (151 aa), FASTA scores: opt: 533, E(): 2.7e-29, (58.15% identity in 129 aa overlap); Q9C5C8|AT4G21860 hypothetical 22.0 KDA protein from Arabidopsis thaliana (Mouse-ear cress) (202 aa), FASTA scores: opt: 490, E(): 2.8e-26, (54.05% identity in 124 aa overlap); P39903|YEAA_ECOLI|B1778|Z2817|ECS2487 hypothetical protein from Escherichia coli strains K12 and O157:H7 (137 aa), FASTA scores: opt: 426, E(): 4.4e-22, (46.8% identity in 126 aa overlap). I6YA00 No intermediary metabolism and respiration P71971 I6YA00 GO:0055114,GO:0008113,GO:0046872 I6YA00 NC_000962.3 Mycobrowser_v5 CDS 3010697 3011368 . - 0 Rv2693c Rv2693c Unknown Probable conserved integral membrane alanine and leucine rich protein Rv2693c, (MTCY05A6.14c), len: 223 aa. Probable conserved integral membrane ala-, leu-rich protein, showing some similarity to O54140|SC2E9.15 hypothetical 29.6 KDA protein from Streptomyces coelicolor (272 aa), FASTA scores: opt: 212, E(): 4.3e-06, (23.5% identity in 247 aa overlap). I6X548 No cell wall and cell processes I6X548 NC_000962.3 Mycobrowser_v5 CDS 2986839 2987615 . + 0 Rv2671 ribD Involved in riboflavin biosynthesis (at the second and third steps). Converts 2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate into 5-amino-6-(ribosylamino)-2,4(1H,3H)-pyrimidinedione 5'-phosphate [catalytic activity 1: 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + H(2)O = 5-amino-6-(5-phosphoribosylamino)uracil + NH(3)] [catalytic activity 2: 5-amino-6-(5-phosphoribitylamino)uracil + NADP(+) = 5-amino-6-(5-phosphoribosylamino)uracil + NADPH]. Possible bifunctional enzyme riboflavin biosynthesis protein RibD: diaminohydroxyphosphoribosylaminopyrimidine deaminase (riboflavin-specific deaminase) + 5-amino-6-(5-phosphoribosylamino)uracil reductase (HTP reductase) Rv2671, (MTCY441.40), len: 258 aa. Possible ribD (alternate gene name: ribG), bifunctional riboflavin biosynthesis protein incuding diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5-amino-6-(5-phosphoribosylamino) uracil reductase, highly similar to O05684|MLC1351.23|ML1340 possible reductase from Mycobacterium leprae (268 aa), FASTA scores: opt: 1211, E(): 3e-68, (72.9% identity in 251 aa overlap). Also weakly similar to others e.g. Q9HWX2|RIBD|PA4056 riboflavin-specific deaminase/reductase from Pseudomonas aeruginosa (373 aa), FASTA scores: opt: 211, E(): 6.3e-06, (30.1% identity in 216 aa overlap); Q9HQA1|RIBG|VNG1256G riboflavin-specific deaminase from Halobacterium sp. strain NRC-1 (220 aa), FASTA scores: opt: 202, E(): 1.5e-05, (27.0% identity in 174 aa overlap); O28272|RIB7_ARCFU|AF2007 putative 5-amino-6-(5-phosphoribosylamino)uracil reductase (HTP reductase) from Archaeoglobus fulgidus (219 aa), FASTA scores: opt: 209, E(): 5.4e-06, (24.15% identity in 211 aa overlap); P25539|RIBD_ECOLI|RIBG|B0414 from Escherichia coli strain K12 (367 aa), FASTA scores: opt: 185, E(): 0.00026, (26.7% identity in 221 aa overlap); etc. But also similar to several hydrolases e.g. Q9X825|SC9B1.05 putative hydrolase from Streptomyces coelicolor (265 aa), FASTA scores: opt: 536, E(): 2.9e-26, (44.25% identity in 235 aa overlap); Q9RKM1|SCD17.10 putative bifunctional enzyme deaminase/reductase from Streptomyces coelicolor (376 aa), FASTA scores: opt: 228, E(): 5.6e-07, (33.5% identity in 188 aa overlap); etc. Equivalent to AAK47060 from Mycobacterium tuberculosis strain CDC1551 (239 aa) but longer 19 aa. Supposed belong to the cytidine and deoxycytidylate deaminases family in the N-terminal section; and to the HTP reductase family in the C-terminal section. P71968 No intermediary metabolism and respiration P71968 P71968 GO:0008835,GO:0055114,GO:0009231,GO:0008703 3.5.4.26,1.1.1.193 P71968 NC_000962.3 Mycobrowser_v5 CDS 2987682 2989268 . + 0 Rv2672 Rv2672 Function unknown; possibly hydrolyzes peptides and/or proteins. Possible secreted protease Rv2672, (MTCY441.41), len: 528 aa. Possible secreted protease, equivalent to O05685|MLC1351.24|ML1339 putative secreted protease from Mycobacterium leprae (525 aa), FASTA scores: opt: 2722, E(): 9.4e-140, (74.45% identity in 528 aa overlap). Also similar to several exported proteinases from Streptomyces and Mycobacteria e.g. Q54399|SLPE proteinase from Streptomyces lividans (513 aa), FASTA scores: opt: 429, E(): 6.8e-16, (26.2% identity in 538 aa overlap); Q9FCK9|2SC3B6.03c peptidase from Streptomyces coelicolor (513 aa), FASTA scores: opt: 421, E(): 1.8e-15, (26.45% identity in 541 aa overlap); Q10508|YM23_MYCTU from Mycobacterium tuberculosis (520 aa), FASTA scores: opt: 349, E(): 1.4e-11, (26.6% identity in 523 aa overlap); etc. Equivalent to AAK47061 from Mycobacterium tuberculosis strain CDC1551 (518 aa) but longer 10 aa. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). Predicted to be an outer membrane protein (See Song et al., 2008). P71969 No intermediary metabolism and respiration P71969 P71969 GO:0008233 3.4.-.- P71969 NC_000962.3 Mycobrowser_v5 CDS 3051806 3052012 . - 0 Rv2738c Rv2738c Function unknown Conserved hypothetical protein Rv2738c, (MTV002.03c), len: 68 aa. Conserved hypothetical protein, equivalent to Q9CCC1|ML0986 hypothetical protein from Mycobacterium leprae (67 aa), FASTA scores: opt: 397, E(): 3.7e-22, (83.6% identity in 67 aa overlap). Also highly similar to O50484|SC4H8.05 hypothetical 7.5 KDA protein from Streptomyces coelicolor (64 aa), FASTA scores: opt: 185, E(): 5.9e-07, (39.7% identity in 63 aa overlap). Second part of the protein is highly similar to C-terminus of upstream ORF O33285|Rv2742c|MTV002.07c conserved hypothetical protein from Mycobacterium tuberculosis (277 aa), FASTA scores: opt: 200, E(): 1.7e-07, (78.4% identity in 37 aa overlap). I6YA47 No conserved hypotheticals O33281 I6YA47 NC_000962.3 Mycobrowser_v5 CDS 2991184 2991936 . - 0 Rv2675c Rv2675c Function unknown Conserved hypothetical protein Rv2675c, (MTCY441.44c), len: 250 aa. Conserved hypothetical protein. C-terminus highly similar to Q50010|U1764Z from Mycobacterium leprae (69 aa), FASTA scores: opt: 284, E(): 4.6e-11, (68.25% identity in 63 aa overlap). Shows some similarity with Q9P3V6|SPAC1348.04 (alias Q9P3E7|Q9P7U5) hypothetical 16.6 KDA protein from Schizosaccharomyces pombe (Fission yeast) (145 aa), FASTA scores: opt: 203, E(): 9.5e-06, (33.05% identity in 118 aa overlap); Q9ZSZ7|BMCT methyl chloride transferase from Batis maritima (230 aa), FASTA scores: opt: 197, E(): 3.3e-05, (28.85% identity in 156 aa overlap); P72459|STSG methyltransferase from Streptomyces griseus (253 aa), FASTA scores: opt: 194, E(): 5.5e-05, (24.45% identity in 229 aa overlap); etc. Also similar to various proteins from Mycobacterium tuberculosis e.g. P71805|Rv1377c|MTCY02B12.11c hypothetical 22.8 KDA protein (212 aa), FASTA scores: opt: 431, E(): 8.3e-20, (39.1% identity in 197 aa overlap); O06426|Rv0560c|MTCY25D10.39c hypothetical 25.9 KDA protein (241 aa), FASTA scores: opt: 379, E(): 1.6e-16, (35.95% identity in 178 aa overlap); O69667|Rv3699|MTV025.047 putative methyltransferase (233 aa), FASTA scores: opt: 297, E(): 2e-11, (30.55% identity in 193 aa overlap); etc. I6Y1G8 No conserved hypotheticals P71972 I6Y1G8 GO:0008168,GO:0008152 I6Y1G8 NC_000962.3 Mycobrowser_v5 CDS 2991933 2992628 . - 0 Rv2676c Rv2676c Function unknown Conserved protein Rv2676c, (MTCY441.45c), len: 231 aa. Conserved protein, equivalent to Q9CCB2|ML1045 (alias Q50009|U1764Y but longer 66 aa) hypothetical protein from Mycobacterium leprae (231 aa), FASTA scores: opt: 1401, E(): 8.7e-88, (87.45% identity in 231 aa overlap). Also highly similar to O69830|SC1B5.02 hypothetical 28.1 KDA protein from Streptomyces coelicolor (243 aa), FASTA scores: opt: 915, E(): 7.7e-55, (61.25% identity in 222 aa overlap); and similar to others e.g. Q9RUB0|DR1481 conserved hypothetical protein from Deinococcus radiodurans (289 aa), FASTA scores: opt: 327, E(): 6.1e-15, (31.8% identity in 176 aa overlap); Q97WP2|SSO2169 hypothetical protein from Sulfolobus solfataricus (223 aa), FASTA scores: opt: 285, E(): 3.4e-12, (31.3% identity in 163 aa overlap); BAB59947|TVG0805714 hypothetical protein from Thermoplasma volcanium (223 aa), FASTA scores: opt: 206, E(): 7.7e-07, (25.0% identity in 176 aa overlap); etc. P9WL45 No conserved hypotheticals P71973 P9WL45 P9WL45 NC_000962.3 Mycobrowser_v5 CDS 3126240 3127367 . - 0 Rv2819c Rv2819c Unknown Hypothetical protein Rv2819c, (MTCY16B7.23), len: 375 aa. Hypothetical unknown protein (see citations below). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WJF5 No conserved hypotheticals P71633 P9WJF5 P9WJF5 NC_000962.3 Mycobrowser_v5 CDS 3000112 3000609 . + 0 Rv2683 Rv2683 Function unknown Conserved protein Rv2683, (MTCY05A6.04), len: 165 aa. Conserved protein, equivalent, but shorter 19 aa, to Q49999|ML1037|U1764Q hypothetical protein from Mycobacterium leprae (184 aa), FASTA scores: opt: 750, E(): 1.2e-41, (73.8% identity in 164 aa overlap). Shows some similarity with other hypothetical proteins e.g. Q988S9|MLL6611 from Rhizobium loti (Mesorhizobium loti) (232 aa), FASTA scores: opt: 128, E(): 0.25, (25.5% identity in 149 aa overlap); Q9YFL5|APE0233 from Aeropyrum pernix (340 aa), FASTA scores: opt: 123, E(): 0.73, (29.1% identity in 141 aa overlap); BAB60477|TVG1377730 from Thermoplasma volcanium (174 aa), FASTA scores: opt: 118, E(): 0.86, (28.8% identity in 59 aa overlap); etc. I6X540 No conserved hypotheticals O07185 I6X540 I6X540 NC_000962.3 Mycobrowser_v5 CDS 2995115 2995945 . + 0 Rv2679 echA15 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Probable enoyl-CoA hydratase EchA15 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv2679, (MTV010.03), len: 276 aa. Probable echA15, enoyl-CoA hydratase, similar to P53526|ECHC_MYCLE|ECHA12|ML1241|MLCB1610.01|B1170_C2_224 probable enoyl-CoA hydratase from Mycobacterium leprae (294 aa), FASTA scores: opt: 368, E(): 2.5e-16, (32.15% identity in 277 aa overlap). Also highly similar to Q9RXX1|DR0184 from Deinococcus radiodurans (273 aa), FASTA scores: opt: 993, E(): 2.2e-56, (58.15% identity in 263 aa overlap); and similar to many e.g. Q9ETY7|PACA|PAAG from Azoarcus evansii (273 aa), FASTA scores: opt: 396, E(): 3.8e-18, (34.9% identity in 258 aa overlap); O29299|AF0963|FAD-3 from Archaeoglobus fulgidus (259 aa), FASTA scores: opt: 363, E(): 4.7e-16, (30.4% identity in 250 aa overlap); P77467|PAAG_ECOLI|B1394 from Escherichia coli strain W (262 aa), FASTA scores: opt: 357, E(): 1.1e-15, (31.75% identity in 252 aa overlap); etc. Also similar to O53163|ECHC_MYCTU|ECHA12|FADB2|Rv1472|MT1518|MTV007.19 enoyl-CoA hydratase from Mycobacterium tuberculosis (285 aa), FASTA scores: opt: 355, E(): 1.6e-15, (31.3% identity in 265 aa overlap); and O06542|ECHA10|Rv1142c|MTCI65.09c|Z95584 enoyl-CoA hydratase from Mycobacterium tuberculosis (268 aa). Contains PS00166 Enoyl-CoA hydratase/isomerase signature. Belongs to the enoyl-CoA hydratase/isomerase family. I6YA03 No lipid metabolism O53232 I6YA03 GO:0016853,GO:0008152,GO:0004300 4.2.1.17 I6YA03 NC_000962.3 Mycobrowser_v5 CDS 3019814 3020203 . - 0 Rv2705c Rv2705c Function unknown Conserved hypothetical protein Rv2705c, (MTCY05A6.26c), len: 129 aa (unlikely ORF). Conserved hypothetical protein, similar to others e.g. Q9RXR5|DR0242 conserved hypothetical protein from Deinococcus radiodurans (112 aa), FASTA scores: opt: 259, E(): 9.4e-10, (40.5% identity in 116 aa overlap); CAC45122|SMC02246 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (115 aa), FASTA scores: opt: 208, E(): 1.6e-06, (38.3% identity in 107 aa overlap); Q98B88|MLL5682 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (116 aa), FASTA scores: opt: 173, E(): 0.00026, (34.95% identity in 103 aa overlap); etc. O07206 No conserved hypotheticals O07206 O07206 O07206 NC_000962.3 Mycobrowser_v5 CDS 2996739 2998055 . + 0 Rv2681 Rv2681 Function unknown Conserved hypothetical alanine rich protein Rv2681, (MTCY05A6.02), len: 438 aa. Conserved hypothetical ala-rich protein, equivalent to Q50004|ML1040|U1764U hypothetical protein from Mycobacterium leprae (429 aa), FASTA scores: opt: 2146, E(): 1.1e-119, (77.4% identity in 416 aa overlap). Also highly similar to O69858|SC1C3.16c hypothetical 42.5 KDA protein from Streptomyces coelicolor (394 aa), FASTA scores: opt: 1336, E(): 9e-72, (51.6% identity in 405 aa overlap); and with some similarity to ribonucleases D e.g. Q983F2|MLL8354 from Rhizobium loti (Mesorhizobium loti) (383 aa), FASTA scores: opt: 379, E(): 3.9e-15, (31.6% identity in 323 aa overlap); Q9A7L8|CC1704 from Caulobacter crescentus (389 aa), FASTA scores: opt: 370, E(): 1.3e-14, (31.45% identity in 318 aa overlap); CAC45770 from Rhizobium meliloti (Sinorhizobium meliloti) (383 aa), FASTA scores: opt: 331, E(): 2.7e-12, (27.75% identity in 357 aa overlap); etc. I6XF17 No conserved hypotheticals O07183 I6XF17 GO:0005622,GO:0003676,GO:0006139,GO:0008408 I6XF17 NC_000962.3 Mycobrowser_v5 CDS 2998052 2999968 . - 0 Rv2682c dxs1 Involved in the deoxyxylulose-5-phosphate pathway (DXP) of isoprenoidbiosynthesis (at the first step), and in the biosynthetic pathway to thiamine and pyridoxol (at the first step). Catalyzes the acyloin condensation reaction between atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP). Probable 1-deoxy-D-xylulose 5-phosphate synthase Dxs1 (1-deoxyxylulose-5-phosphate synthase) (DXP synthase) (DXPS) Rv2682c, (MTCY05A6.03c), len: 638 aa. Probable dxs1, 1-deoxy-D-xylulose 5-phosphate synthase, equivalent to Q50000|DXS_MYCLE|TKTB|ML1038 1-deoxy-D-xylulose 5-phosphate synthase from Mycobacterium leprae (643 aa), FASTA scores: opt: 3635, E(): 5.6e-209, (86.4% identity in 632 aa overlap). Also highly similar to other Q9X7W3|DXS_STRCO|DXS|SC6A5.17 from Streptomyces coelicolor (656 aa), FASTA scores: opt: 2501, E(): 2e-141, (61.3% identity in 623 aa overlap); Q9K971|DXS_BACHD|DXS|BH2779 from Bacillus halodurans (629 aa), FASTA scores: opt: 1612, E(): 1.8e-88, (41.35% identity in 619 aa overlap); P77488|DXS_ECOLI|DXS|B0420 from Escherichia coli strain K12 (619 aa), FASTA scores: opt: 1511, E(): 1.8e-82, (39.5% identity in 625 aa overlap); etc. Also similar to O50408|Rv3379c|MTV004.37c from Mycobacterium tuberculosis (536 aa). Belongs to the transketolase family. DXS subfamily. Cofactor: thiamine pyrophosphate. Note that previously known as dxs. P9WNS3 No intermediary metabolism and respiration P0A554 P9WNS3 GO:0016114,GO:0009228,GO:0030976,GO:0008661 2.2.1.7 P9WNS3 NC_000962.3 Mycobrowser_v5 CDS 3000614 3001903 . + 0 Rv2684 arsA Thought to be involved in active transport of arsenical compounds across the membrane (export): arsenic resistance by an export mechanism. Responsible for the translocation of the substrate across the membrane. Probable arsenic-transport integral membrane protein ArsA Rv2684, (MTCY05A6.05), len: 429 aa. Probable arsA, arsenic-transport integral membrane protein, equivalent to P46838|AG45_MYCLE|ML1036 46 KDA probable integral membrane protein (antigen 45, a transmembrane protein related to arsenical pumps) from Mycobacterium leprae (429 aa), FASTA scores: opt: 2067, E(): 9.9e-118, (74.05% identity in 428 aa overlap); and upstream orf O07187|YQ85_MYCTU|ARSB|Rv2685|MT2759|MTCY05A6.06 probable integral membrane 45.2 KDA protein ARSB from Mycobacterium tuberculosis (428 aa), FASTA scores: opt: 2148, E(): 1.3e-122, (76.58% identity in 427 aa overlap). Also highly similar to other proteins e.g. Q9UY19|PAB1107 transport protein from Pyrococcus abyssi (425 aa), FASTA scores: opt: 1109, E(): 8.3e-60, (41.45% identity in 427 aa overlap); O59575|PH1912 hypothetical 46.0 KDA protein from Pyrococcus horikoshii (424 aa), FASTA scores: opt: 1101, E(): 2.5e-59, (41.95% identity in 429 aa overlap); Q9KDI2|BH1231 hypothetical 46.0 KDA protein from Bacillus halodurans (428 aa), FASTA scores: opt: 1018, E(): 2.7e-54, (38.9% identity in 427 aa overlap); etc. Belongs to the NADC/P/PHO87 family of transporters, P subfamily (ARS family). P9WPD9 No cell wall and cell processes P0A606 P9WPD9 GO:0015137,GO:0015746,GO:0016021,GO:0005886,GO:0055085,GO:0015105 P9WPD9 NC_000962.3 Mycobrowser_v5 CDS 3003280 3004038 . - 0 Rv2686c Rv2686c Thought to be involved in active transport of unidentified antibiotic across the membrane (export): antibiotic resistance by an export mechanism. Responsible for the translocation of the substrate across the membrane. Antibiotic-transport integral membrane leucine and alanine and valine rich protein ABC transporter Rv2686c, (MTCY05A6.07c), len: 252 aa. Antibiotic-transport integral membrane leu-, ala-, val-rich protein ABC transporter (see citation below). The region from aa ~115 to 160 is highly similar to N-terminus of Q49998|U1764P hypothetical protein from Mycobacterium leprae (53 aa), FASTA scores: opt: 151, E(): 0.011, (58.15% identity in 43 aa overlap). Shows some similarity with membrane proteins e.g. AAK75541|SP1447 membrane protein from Streptococcus pneumoniae (298 aa), FASTA scores: opt: 139, E(): 0.21, (29.65% identity in 135 aa overlap); Q9K4C9|2SC6G5.26c putative ABC transporter integral membrane subunit from Streptomyces coelicolor (249 aa), FASTA scores: opt: 138, E(): 0.21, (26.9% identity in 253 aa overlap); Q53627|MTRB membrane protein involved in mithramycin resistance from Streptomyces argillaceus (233 aa), FASTA scores: opt: 136, E(): 0.27, (26.7% identity in 191 aa overlap); etc. P9WJB3 No cell wall and cell processes P9WJB3 GO:0005886,GO:0046677,GO:0006810,GO:0016021 P9WJB3 NC_000962.3 Mycobrowser_v5 CDS 3014663 3014965 . - 0 Rv2699c Rv2699c Function unknown Conserved hypothetical protein Rv2699c, (MTCY05A6.20c), len: 100 aa. Conserved hypothetical protein, very equivalent to Q49990|ML1026|U1764J hypothetical protein from Mycobacterium leprae (100 aa), FASTA scores: opt: 632, E(): 7.7e-36, (96.0% identity in 100 aa overlap). Also highly similar to O54130|SC2E9.05 hypothetical 11.0 KDA protein from Streptomyces coelicolor (98 aa), FASTA scores: opt: 465, E(): 1.1e-24, (71.45% identity in 98 aa overlap). I6YA17 No conserved hypotheticals I6YA17 NC_000962.3 Mycobrowser_v5 CDS 3021839 3022285 . + 0 Rv2709 Rv2709 Unknown Probable conserved transmembrane protein Rv2709, (MTCY05A6.30), len: 148 aa. Probable conserved transmembrane protein, equivalent to Q9CCB4|ML1015 (alias Q49983|U1764B but extended in N-terminus) possible conserved membrane protein from Mycobacterium leprae (139 aa), FASTA scores: opt: 578, E(): 5.5e-31, (70.75% identity in 123 aa overlap). Shows also similarity with Q9RJ48|SCI8.05 putative integral membrane protein from Streptomyces coelicolor (159 aa), FASTA scores: opt: 119, E(): 0.57, (31.95% identity in 119 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). I6YA25 No cell wall and cell processes O07210 I6YA25 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 3005845 3007062 . - 0 Rv2689c Rv2689c Function unknown Conserved alanine and valine and glycine rich protein Rv2689c, (MTCY05A6.10c), len: 405 aa (other less probable starts possible). Conserved ala-, val-, gly-rich protein, similar to O54099|SC10A5.06 hypothetical 49.5 KDA protein from Streptomyces coelicolor (458 aa), FASTA scores: opt: 455, E(): 2.7e-20, (38.35% identity in 417 aa overlap); and shows weak similarity in part with several methyltransferases e.g. Q9X0H9|TM1094 putative RNA methyltransferase from Thermotoga maritima (439 aa), FASTA scores: opt: 306, E(): 3e-11, (25.9% identity in 436 aa overlap); AK79403|CAC1435 S-adenosylmethionine-dependent methyltransferases from Clostridium acetobutylicum (456 aa), FASTA scores: opt: 294, E(): 1.6e-10, (23.4% identity in 449 aa overlap); Q9A8M7|CC1326 RNA methyltransferase from Caulobacter crescentus (415 aa), FASTA scores: opt: 247, E(): 1.1e-07, (28.4% identity in 433 aa overlap); etc. Equivalent to AAK47078 from Mycobacterium tuberculosis strain CDC1551 (434 aa) but shorter 29 aa. O07191 No conserved hypotheticals O07191 O07191 GO:0006396,GO:0008173 O07191 NC_000962.3 Mycobrowser_v5 CDS 3007236 3009209 . - 0 Rv2690c Rv2690c Unknown Probable conserved integral membrane alanine and valine and leucine rich protein Rv2690c, (MTCY05A6.11c), len: 657 aa. Probable conserved integral membrane ala-, val-, leu-rich protein, highly similar to others e.g. O54098|SC10A5.05 putative membrane protein from Streptomyces coelicolor (691 aa), FASTA scores: opt: 2007, E(): 1.6e-116, (62.35% identity in 669 aa overlap); O69917|SC3C8.04c putative integral membrane protein from Streptomyces coelicolor (644 aa), FASTA scores: opt: 923, E(): 1.7e-49, (35.3% identity in 669 aa overlap); AAK78253|CAC0272 amino acid transporter from Clostridium acetobutylicum (620 aa), FASTA scores: opt: 674, E(): 4.1e-34, (36.55% identity in 640 aa overlap); etc. I6Y1H7 No cell wall and cell processes I6Y1H7 I6Y1H7 NC_000962.3 Mycobrowser_v5 CDS 3009344 3010027 . + 0 Rv2691 ceoB Part of a potassium transport system. TRK system potassium uptake protein CeoB Rv2691, (MTCY05A6.12), len: 227 aa. CeoB (alternate gene name: trkA), TRK system potassium uptake protein (see citation below), highly similar to others e.g. Q53949|TRKA_STRCO|SC2E9.17c from Streptomyces coelicolor (223 aa), FASTA scores: opt: 781, E(): 5.8e-42, (53.2% identity in 220 aa overlap); O27333|TRKA_METTH|MTH1265 from Methanobacterium thermoautotrophicum (216 aa), FASTA scores: opt: 287, E(): 5.3e-11, (27.0% identity in 211 aa overlap); O54141|SC2E9.16c from Streptomyces coelicolor (226 aa), FASTA scores: opt: 269, E(): 7.3e-10, (29.9% identity in 214 aa overlap); etc. Also similar to upstream orf O07194|CEOC|TRKA_MYCTU|TRKA|TRKB|Rv2692|MT2766|MTCY05A6.13 TRK system potassium uptake protein from Mycobacterium tuberculosis (220 aa), FASTA scores: opt: 259, E(): 3e-09, (26.55% identity in 226 aa overlap). Contains a motif common to NAD+ binding pockets (see citation below). Belongs to the TrkA family. I6XF25 No cell wall and cell processes Q7BV63 I6XF25 GO:0003824,GO:0008324,GO:0008152,GO:0006813 I6XF25 NC_000962.3 Mycobrowser_v5 CDS 3056420 3057232 . - 0 Rv2743c Rv2743c Unknown Possible conserved transmembrane alanine rich protein Rv2743c, (MTV002.08c), len: 270 aa. Possible conserved transmembrane ala-rich protein, equivalent to Q49833|MLCB33.04c|B2235_C1_148 unknown protein from Mycobacterium leprae (123 aa), FASTA scores: opt: 639, E(): 3.3e-31, (74.8% identity in 123 aa overlap). I6YA50 No cell wall and cell processes I6YA50 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 3312953 3313201 . - 0 Rv2960c Rv2960c Unknown Hypothetical protein Rv2960c, (MT3036, MTCY349.28), len: 82 aa. Hypothetical unknown protein, equivalent to AAK47362 from Mycobacterium tuberculosis strain CDC1551 (116 aa) but shorter 34 aa. Shortened version of MTCY349.28 avoiding overlap. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P95133 No conserved hypotheticals P95133 NC_000962.3 Mycobrowser_v5 CDS 3011916 3012623 . + 0 Rv2695 Rv2695 Function unknown Conserved hypothetical alanine rich protein Rv2695, (MTCY05A6.16), len: 235 aa. Conserved hypothetical ala-rich protein, equivalent to Q49994|ML1030|U1764L hypothetical protein from Mycobacterium leprae (232 aa), FASTA scores: opt: 1166, E(): 6.3e-63, (76.95% identity in 230 aa overlap). Also shows some similarity with other hypothetical proteins e.g. Q986S2|MLR7232 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (277 aa), FASTA scores: opt: 150, E(): 0.059, (33.55% identity in 173 aa overlap); CAC47772|SMC03810 hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (269 aa), FASTA scores: opt: 143, E(): 0.15, (28.05% identity in 228 aa overlap); Q9A5N6|CC2411 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase from Caulobacter crescentus (393 aa), FASTA scores: opt: 138, E(): 0.41, (26.45% identity in 238 aa overlap); etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Nucleotide position 3012293 in the genome sequence has been corrected, A:G resulting in T126T. I6Y1I1 No conserved hypotheticals O07197 I6Y1I1 I6Y1I1 NC_000962.3 Mycobrowser_v5 CDS 3012829 3013608 . - 0 Rv2696c Rv2696c Function unknown Conserved alanine and glycine and valine rich protein Rv2696c, (MTCY05A6.17c), len: 259 aa. Conserved ala-, gly-, val-rich protein, equivalent (but shorter 18 aa) to Q49993|ML1029|U1764K hypothetical protein from Mycobacterium leprae (273 aa), FASTA scores: opt: 1174, E(): 2.1e-63, (70.6% identity in 262 aa overlap). Also similar to O54135|SC2E9.10 from Streptomyces coelicolor (250 aa), FASTA scores: opt: 213, E(): 9.8e-06, (28.25% identity in 255 aa overlap); and showing weak similarity with other proteins. I6XF31 No conserved hypotheticals O07198 I6XF31 NC_000962.3 Mycobrowser_v5 CDS 3014173 3014658 . + 0 Rv2698 Rv2698 Unknown Probable conserved alanine rich transmembrane protein Rv2698, (MTCY05A6.19), len: 161 aa. Probable conserved ala-rich transmembrane protein, equivalent to Q49991|ML1027|U1764I possible membrane protein from Mycobacterium leprae (157 aa), FASTA scores: opt: 886, E(): 1.1e-49, (78.9% identity in 161 aa overlap). Also similar to O54132|SC2E9.07c hypothetical 16.5 KDA protein from Streptomyces coelicolor (154 aa), FASTA scores: opt: 230, E(): 7.1e-08, (35.7% identity in 154 aa overlap). I6X552 No cell wall and cell processes O07200 I6X552 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 3015863 3016735 . - 0 Rv2701c suhB Involved in inositol phosphate metabolism. It is responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides. Key enzyme of the phosphatidyl inositol signaling pathway. [catalytic activity: myo-inositol 1-phosphate + H(2)O = myo-inositol + phosphate] Inositol-1-monophosphatase SuhB Rv2701c, (MTCY05A6.22c), len: 290 aa. SuhB, inositol-1-monophosphatase. Equivalent to AAK47090 from Mycobacterium tuberculosis strain CDC1551 (277 aa) but longer 13 aa. Contains PS00630 Inositol monophosphatase family signatures 1 and 2 (PS00629 and PS00630). Belongs to the inositol monophosphatase family. Cofactor: Mg2+. Activity is inhibited by Li+ but not when Leu81 is mutated (See Nigou et al., 2002). Mg2+ promotes dimerization; Li+ amplifies this effect but does not promote dimerization on its own (See Brown et al., 2007). P9WKI9 No intermediary metabolism and respiration 2Q74 P65165 P9WKI9 GO:0046872,GO:0008934 3.1.3.25 P9WKI9 NC_000962.3 Mycobrowser_v5 CDS 3017835 3019421 . + 0 Rv2703 sigA The sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released. This is the primary sigma-factor of this bacteria. Supposedly involved in the housekeeping regulons. RNA polymerase sigma factor SigA (sigma-A) Rv2703, (MTCY05A6.24), len: 528 aa. SigA (formerly named mysA, and also known as rpoV or rpoD), RNA polymerase sigma factor (see citations below), equivalent (but shorter 55 aa) to Q9S5K3|RPOT (alias Q59532) RNA polymerase sigma factor from Mycobacterium leprae (576 aa), FASTA scores: opt: 2638, E(): 8.6e-115, (80.35% identity in 535 aa overlap). Also similar to others e.g. Q59552|MYSA from Mycobacterium smegmatis (466 aa), FASTA scores: opt: 2259, E(): 2.3e-97, (76.5% identity in 528 aa overlap); Q45302|SIGA from Corynebacterium glutamicum (Brevibacterium flavum) (497 aa), FASTA scores: opt: 1972, E(): 4.3e-84, (67.35% identity in 505 aa overlap); Q59813|HRDB from Streptomyces aureofaciens (525 aa), FASTA scores: opt: 1654, E(): 2.1e-69, (67.5% identity in 468 aa overlap); etc. Contains sigma-70 family signatures 1 and 2 (PS00715 and PS00716). Belongs to the sigma-70 factor family. P9WGI1 No information pathways P0A602 P9WGI1 GO:0016987,GO:0003700,GO:0006352,GO:0006355 P9WGI1 NC_000962.3 Mycobrowser_v5 CDS 3019458 3019886 . + 0 Rv2704 Rv2704 Function unknown Conserved protein Rv2704, (MTCY05A6.25), len: 142 aa. Conserved protein, highly similar (but shorter 25 aa) to Q9RYB7|DR0033 conserved hypothetical protein from Deinococcus radiodurans (157 aa), FASTA scores: opt: 381, E(): 1.5e-17, (54.85% identity in 124 aa overlap); and highly similar to various proteins e.g. CAC47758|SMC03796 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (126 aa), FASTA scores: opt: 302, E(): 1.4e-12, (46.6% identity in 126 aa overlap); Q98E55|MLL4402 from Rhizobium loti (Mesorhizobium loti) (130 aa), FASTA scores: opt: 252, E(): 2.1e-09, (40.15% identity in 127 aa overlap); Q9K3V5|SCD10.21 putative acetyltransferase from Streptomyces coelicolor (291 aa), FASTA scores: opt: 247, E(): 8.7e-09, (41.3% identity in 138 aa overlap) (homology only in N-terminal region); etc. Belongs to the YJGF/YER057C/UK114 protein family. I6YA21 No conserved hypotheticals 3I7T O07205 I6YA21 I6YA21 NC_000962.3 Mycobrowser_v5 CDS 3020573 3021547 . + 0 Rv2707 Rv2707 Unknown Probable conserved transmembrane alanine and leucine rich protein Rv2707, (MTCY05A6.28), len: 324 aa. Probable conserved transmembrane ala-, leu-rich protein, equivalent to Q49985|ML1017|U1764D possible conserved integral membrane protein from Mycobacterium leprae (330 aa), FASTA scores: opt: 1617, E(): 2.5e-91, (75.4% identity in 325 aa overlap). Also similar to other membrane proteins e.g. Q9ADF6|SCBAC1A6.31 putative integral membrane protein from Streptomyces coelicolor (344 aa), FASTA scores: opt: 593, E(): 5.9e-29, (36.2% identity in 268 aa overlap); Q99SZ8|SA1699 hypothetical protein (similar to transporter) from Staphylococcus aureus subsp. aureus N315 (405 aa), FASTA scores: opt: 318, E(): 3.7e-12, (27.9% identity in 265 aa overlap); O34437|YFKH hypothetical protein (similar to transporter) from Bacillus subtilis (275 aa), FASTA scores: opt: 309, E(): 9.7e-12, (29.3% identity in 263 aa overlap); etc. I6YE67 No conserved hypotheticals O07208 I6YE67 GO:0004540,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 3023565 3024257 . + 0 Rv2711 ideR Transcriptional regulatory protein (repressor and activator), iron-binding repressor of siderophore biosynthesis and iron uptake. Seems to regulate a variety of genes encoding a variety of proteins e.g. transporters, proteins involved in siderophore synthesis and iron storage, members of the PE/PPE family, enzymes involved in lipid metabolism, transcriptional regulatory proteins, etc. Also activator of BFRA|Rv1876 gene. Iron-dependent repressor and activator IdeR Rv2711, (MTCY05A6.32), len: 230 aa. IdeR (formerly known as dtxR), iron dependent repressor and activator (see citations below), equivalent to Q9CCB5|ML1013 iron dependent repressor from Mycobacterium leprae (230 aa), FASTA scores: opt: 1365, E(): 3.8e-77, (90.0% identity in 230 aa overlap). Also highly similar to others e.g. Q50379|DTXR from Mycobacterium smegmatis (233 aa), FASTA scores: opt: 1291, E(): 1.4e-72, (86.1% identity in 230 aa overlap); Q9F7T3|IDER from Corynebacterium equii (Rhodococcus equi) (230 aa), FASTA scores: opt: 1130, E(): 1.2e-62, (74.8% identity in 230 aa overlap); P33120|DTXR_CORDI from Corynebacterium diphtheriae (226 aa), FASTA scores: opt: 803, E(): 1.6e-42, (57.85% identity in 230 aa overlap); etc. Belongs to the fur family. P9WMH1 No regulatory proteins 1B1B,1FX7,1U8R,2ISY,2ISZ,2IT0 P0A672 P9WMH1 GO:0005506,GO:0006355,GO:0006350,GO:0003700,GO:0005737 P9WMH1 NC_000962.3 Mycobrowser_v5 CDS 3024270 3025328 . - 0 Rv2712c Rv2712c Unknown Hypothetical protein Rv2712c, (MTCY05A6.33c), len: 352 aa. Hypothetical unknown ala-, leu-rich protein. I6YE70 No conserved hypotheticals I6YE70 I6YE70 NC_000962.3 Mycobrowser_v5 CDS 3049052 3051424 . - 0 Rv2737c recA Involved in regulation of nucleotide excision repair, in genetic recombination, and in induction of the sos response. Endonuclease which can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAS. It interacts with LEXA|Rv2720 causing its activation and leading to its autocatalytic cleavage. RecA protein (recombinase A) [contains: endonuclease PI-MTUI (MTU RecA intein)]. Rv2737c, (MTV002.02c), len: 790 aa. RecA, recombinase a (see citations below), equivalent to Q59560|RECA_MYCSM RECA protein from Mycobacterium smegmatis (349 aa), FASTA scores: opt: 1495, E(): 1.9e-79, (93.15% identity in 249 aa overlap); and P35901|RECA_MYCLE|ML0987 RECA protein from Mycobacterium leprae (711 aa), FASTA scores: opt: 1217, E(): 4.5e-63, (46.7% identity in 814 aa overlap). Also highly similar to many e.g. Q9REV6|RECA_AMYMD from Amycolatopsis mediterranei (Nocardia mediterranei) (348 aa), FASTA scores: opt: 1450, E(): 7.6e-77, (89.25% identity in 251 aa overlap); P42442|RECA_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (376 aa), FASTA scores: opt: 1355, E(): 2.6e-71, (76.55% identity in 273 aa overlap); P41054|RECA_STRAM from Streptomyces ambofaciens (372 aa), FASTA scores: opt: 1347, E(): 7.6e-71, (82.1% identity in 246 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00321 recA signature, and PS00881 Protein splicing signature. Belongs to the RecA family. This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation. Belongs to the homing endonuclease family in the intein section. P9WHJ3 No information pathways 1G18,1G19,1MO3,1MO4,1MO5,1MO6,2IMZ,2IN0,2IN8,2IN9,3IFJ,3IGD,2L8L P0A5U4 P9WHJ3 GO:0006281,GO:0008094,GO:0009432,GO:0005737,GO:0003684,GO:0004519,GO:0016539,GO:0006314,GO:0003697,GO:0005524 3.1.-.- P9WHJ3 NC_000962.3 Mycobrowser_v5 CDS 3062505 3062819 . + 0 Rv2749 Rv2749 Function unknown Conserved protein Rv2749, (MTV002.14), len: 104 aa. Conserved protein, showing some similarity with Q9I1R9|PA2198 hypothetical protein from Pseudomonas aeruginosa (114 aa), FASTA scores: opt: 157, E(): 0.00081, (35.0% identity in 100 aa overlap); and O86332|Rv0793|MTV042.03 hypothetical 11.2 KDA protein from Mycobacterium tuberculosis (101 aa), FASTA scores: opt: 143, E(): 0.0062, (26.9% identity in 93 aa overlap). O33291 No conserved hypotheticals O33291 O33291 GO:0005737,GO:0016491,GO:0017000 NC_000962.3 Mycobrowser_v5 CDS 3028098 3029123 . + 0 Rv2715 Rv2715 Function unknown; probably involved in cellular metabolism. Possible hydrolase Rv2715, (MTCY05A6.36), len: 341 aa. Possible hydrolase, showing some similarity with other hydrolases e.g. Q9I5B0|PA0829 probable hydrolase from Pseudomonas aeruginosa (313 aa), FASTA scores: opt: 336, E(): 9.9e-14, (28.05% identity in 289 aa overlap); BAB55888 hydrolase (fragment) from Terrabacter sp. DBF63 (319 aa), FASTA scores: opt: 326, E(): 4.2e-13, (27.95% identity in 290 aa overlap); O52866|CEH|eh soluble epoxide hydrolase from Corynebacterium SP (285 aa), FASTA scores: opt: 325, E(): 4.4e-13, (29.95% identity in 284 aa overlap); etc. Also shows some similarity to P96811|EPHF|Rv0134|MTCI5.08 hypothetical 33.8 KDA protein from Mycobacterium tuberculosis (300 aa), FASTA scores: E(): 1.8e-10, (27.7% identity in 271 aa overlap). Contains lipases, serine active site motif (PS00120). P9WNH3 No intermediary metabolism and respiration P0A572 P9WNH3 GO:0016787 P9WNH3 NC_000962.3 Mycobrowser_v5 CDS 3123625 3123966 . - 0 Rv2816c Rv2816c Function unknown Conserved hypothetical protein Rv2816c, (MTCY16B7.27), len: 113 aa. Conserved hypothetical protein, highly similar in part to N-terminus of several proteins e.g. O28403|AF1876 conserved hypothetical protein from Archaeoglobus fulgidus (94 aa), FASTA scores: opt: 137, E(): 0.0022, (47.55% identity in 61 aa overlap); Q97Y85|SSO8090 hypothetical protein from Sulfolobus solfataricus (88 aa), FASTA scores: opt: 124, E(): 0.02, (37.3% identity in 59 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WPJ3 No conserved hypotheticals P71637 P9WPJ3 P9WPJ3 NC_000962.3 Mycobrowser_v5 CDS 3029172 3029858 . + 0 Rv2716 Rv2716 Function unknown Conserved protein Rv2716, (MTCY05A6.37), len: 228 aa. Conserved protein, similar to other proteins e.g. Q9RKR0|SCC75A.14 hypothetical 23.3 KDA protein from Streptomyces coelicolor (214 aa), FASTA scores: opt: 447, E(): 4e-22, (44.1% identity in 220 aa overlap); Q9HHG6|PHZF|VNG6408G phenazine biosynthetic protein from Halobacterium sp. strain NRC-1 (299 aa), FASTA scores: opt: 201, E(): 6.1e-06, (30.4% identity in 148 aa overlap) (similarity only at N-terminus); P73125|SLR1019 hypothetical 34.1 KDA protein from Synechocystis sp. strain PCC 6803 (314 aa), FASTA scores: opt: 196, E(): 1.4e-05, (28.5% identity in 298 aa overlap); etc. P9WL43 No conserved hypotheticals P0A5G7 P9WL43 GO:0003824,GO:0009058 P9WL43 NC_000962.3 Mycobrowser_v5 CDS 3029867 3030361 . - 0 Rv2717c Rv2717c Function unknown Conserved protein Rv2717c, (MTCY05A6.38c), len: 164 aa. Conserved protein, equivalent to Q9CCB8|ML1006 (alias Q49838 but shortened N-terminus) hypothetical protein from Mycobacterium leprae (161 aa), FASTA scores: opt: 797, E(): 2.3e-46, (73.8% identity in 164 aa overlap). Also highly similar to other eukaryotic proteins e.g. O64527|YUP8H12R.14 hypothetical protein from Arabidopsis thaliana (Mouse-ear cress) (166 aa), FASTA scores: opt: 393, E(): 2.3e-19, (42.4% identity in 158 aa overlap); Q9Y325 CGI-36 protein from Homo sapiens (Human) (165 aa), FASTA scores: opt: 294, E(): 9.5e-13, (33.95% identity in 159 aa overlap); etc. P9WFG7 No conserved hypotheticals 2FR2 O07216 P9WFG7 GO:0006810 P9WFG7 NC_000962.3 Mycobrowser_v5 CDS 3030413 3030877 . - 0 Rv2718c nrdR Involved in transcriptional mechanism Probable transcriptional regulatory protein NrdR Rv2718c, (MTCY05A6.39c), len: 154 aa. Probable nrdR, transcriptional regulatory protein, equivalent to Q49844|ML1005|U2235A|B2235_C2_209 hypothetical 17.3 KDA protein from Mycobacterium leprae (154 aa), FASTA scores: opt: 937, E(): 1.5e-52, (92.7% identity in 151 aa overlap). Highly similar to O86848|NRDR_STRCL putative regulatory protein from Streptomyces clavuligerus (172 aa), FASTA scores: opt: 750, E(): 1.1e-40, (73.65% identity in 148 aa overlap); O69980|SC4H2.25 hypothetical protein from Streptomyces coelicolor (182 aa), FASTA scores: opt: 725, E(): 4.6e-39, (73.1% identity in 145 aa overlap); Q9KPU0|VC2272 hypothetical protein from Vibrio cholerae (156 aa), FASTA scores: opt: 462, E(): 1.8e-22, (47.3% identity in 148 aa overlap); etc. P9WIZ1 No regulatory proteins P67313 P9WIZ1 GO:0003677,GO:0046872,GO:0045449,GO:0006350,GO:0016564,GO:0005524 NC_000962.3 Mycobrowser_v5 CDS 3105056 3105619 . - 0 Rv2796c lppV Unknown Probable conserved lipoprotein LppV Rv2796c, (MTV002.61c, MTCY16B7.47), len: 187 aa. Probable lppV, conserved lipoprotein, similar to others from Mycobacterium tuberculosis e.g. P95009|LPPB|Rv2544|MTCY159.12c probable conserved lipoprotein (220 aa), FASTA scores: opt: 168, E(): 0.00066, (22.45% identity in 196 aa overlap); and P95010|LPPA|RV2543|MTCY159.13c probable conserved lipoprotein (219 aa), FASTA scores: opt: 165, E(): 0.001, (23.1% identity in 199 aa overlap). P71655 No cell wall and cell processes P71655 P71655 NC_000962.3 Mycobrowser_v5 CDS 3036131 3037291 . - 0 Rv2724c fadE20 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE20 Rv2724c, (MTCY154.04c), len: 386 aa. Probable fadE20, acyl-CoA dehydrogenase, highly similar to many e.g. Q9X7Y2|SC6A5.36 from Streptomyces coelicolor (382 aa), FASTA scores: opt: 1583, E(): 6.9e-94, (62.7% identity in 378 aa overlap); Q9HVY0|PA4435 from Pseudomonas aeruginosa (381 aa), FASTA scores: opt: 1468, E(): 1.6e-86, (57.65% identity in 380 aa overlap); Q9ABZ1|CC0079 from Caulobacter crescentus (391 aa), FASTA scores: opt: 1298, E(): 1.2e-75, (51.9% identity in 391 aa overlap); etc. Also similar to many other Mycobacterium tuberculosis proteins e.g. O06164|FADE19|Rv2500c|MTCY07A7.06c acyl-CoA dehydrogenase (394 aa) (34.3% identity in 382 aa overlap). Contains acyl-CoA dehydrogenases signature 2 (PS00073). Belongs to the acyl-CoA dehydrogenases family. O33229 No lipid metabolism O33229 O33229 GO:0003995,GO:0055114,GO:0050660 1.3.99.- O33229 NC_000962.3 Mycobrowser_v5 CDS 3032520 3034619 . - 0 Rv2721c Rv2721c Unknown Possible conserved transmembrane alanine and glycine rich protein Rv2721c, (MTCY05A6.42c, MTCY154.01c), len: 699 aa. Possible conserved transmembrane ala-, gly-rich protein, equivalent to Q49837|ML1002|U2235I possible conserved membrane protein from Mycobacterium leprae (687 aa), FASTA scores: opt: 2703, E(): 6.6e-135, (60.3% identity in 713 aa overlap). Shows some similaity to Q01377|CSP1 PS1 protein precursor (secreted protein) from Corynebacterium glutamicum (Brevibacterium flavum) (657 aa), FASTA scores: opt: 276, E(): 3.8e-07, (29.4% identity in 272 aa overlap); and Q9KIJ0 Rv2721c-like protein from Mycobacterium paratuberculosis (246 aa), FASTA scores: opt: 178, E(): 0.025, (37.5% identity in 120 aa overlap). I6XF52 No cell wall and cell processes O07219 I6XF52 GO:0016021 I6XF52 NC_000962.3 Mycobrowser_v5 CDS 3076894 3078078 . - 0 Rv2768c PPE43 Function unknown PPE family protein PPE43 Rv2768c, (MTV002.33c), len: 394 aa. PPE43, Member of the Mycobacterium tuberculosis PPE family, highly similar to many e.g. upstream ORF O33312|Rv2770c|MTV002.35c (402 aa), FASTA scores: opt: 1135, E(): 6.1e-51, (62.15% identity in 391 aa overlap); and P96362|Rv1039c|MTCY10G2.10 from M. tuberculosis (391 aa), FASTA scores: opt: 1721, E(): 6.8e-81, (70.35% identity in 398 aa overlap). Equivalent to AAK47157 from Mycobacterium tuberculosis strain CDC1551 (462 aa) but shorter 68 aa. Q79FA9 No PE/PPE Q79FA9 Q79FA9 Q79FA9 NC_000962.3 Mycobrowser_v5 CDS 3038931 3039800 . - 0 Rv2726c dapF Involved in biosynthesis of lysine from aspartate semialdehyde (at the sixth step) [catalytic activity: ll-2,6-diaminoheptanedioate = MESO-diaminoheptanedioate]. Probable diaminopimelate epimerase DapF (DAP epimerase) Rv2726c, (MTCY154.06c), len: 289 aa. Probable dapF, diaminopimelate epimerase, equivalent to P46814|DAPF_MYCLE|ML0996|B2235_C3_233 diaminopimelate epimerase from Mycobacterium leprae (296 aa), FASTA scores: opt: 1488, E(): 2.1e-83, (76.05% identity in 292 aa overlap). Also highly similar to O69969|DAPF_STRCO|SC4H2.14 from Streptomyces coelicolor (289 aa), FASTA scores: opt: 439, E(): 1.4e-19, (45.6% identity in 296 aa overlap); and similar to many e.g. O29511|DAPF_ARCFU|AF0747 from Archaeoglobus fulgidus (280 aa), FASTA scores: opt: 310, E(): 9.7e-12, (33.8% identity in 296 aa overlap); Q51564|DAPF_PSEAE|PA5278 from Pseudomonas aeruginosa (276 aa), FASTA scores: opt: 272, E(): 2e-09, (30.15% identity in 292 aa overlap); P08885|DAPF_ECOLI|B3809 from Escherichia coli strain K12 (274 aa), FASTA scores: opt: 266, E(): 4.5e-09, (30.4% identity in 296 aa overlap); etc. Belongs to the diaminopimelate epimerase family. P9WP19 No intermediary metabolism and respiration 3FVE P63897 P9WP19 GO:0008837,GO:0009089,GO:0005737 5.1.1.7 P9WP19 NC_000962.3 Mycobrowser_v5 CDS 3040766 3041461 . - 0 Rv2728c Rv2728c Function unknown Conserved alanine rich protein Rv2728c, (MTCY154.08c), len: 231 aa. Conserved ala-rich protein, equivalent to Q49835|ML0994|B2235_C1_162 hypothetical protein from Mycobacterium leprae (232 aa), FASTA scores: opt: 1037, E(): 1.2e-54, (68.55% identity in 232 aa overlap). Also similar to O69964|SC4H2.09 from Streptomyces coelicolor (237 aa), FASTA scores: opt: 300, E(): 7.7e-11, (32.8% identity in 241 aa overlap); and some similarity with other proteins e.g. Q14234|ELN elastin from Homo sapiens (Human) (757 aa), FASTA scores: opt: 161, E(): 0.03, (30.6% identity in 242 aa overlap); P55488|Y4IE hypothetical 15.4 KDA protein from Rhizobium sp. strain NGR234 (135 aa), FASTA scores: opt: 147, E(): 0.061, (34.95% identity in 123 aa overlap). Shows also some similarity with P71657|Rv1387|MTCY21B4.04 hypothetical protein from Mycobacterium tuberculosis (539 aa), FASTA scores: opt: 159, E(): 0.035, (34.8% identity in 135 aa overlap). I6X579 No conserved hypotheticals I6X579 I6X579 NC_000962.3 Mycobrowser_v5 CDS 3041570 3042475 . - 0 Rv2729c Rv2729c Unknown Probable conserved integral membrane alanine valine and leucine rich protein Rv2729c, (MTCY154.09c), len: 301 aa. Probable conserved integral membrane ala-, val-, leu-rich protein, similar to P42459|YLEU_CORGL hypothetical 29.6 KDA protein from Corynebacterium glutamicum (Brevibacterium flavum)(270 aa), FASTA scores: opt: 365, E(): 4.7e-15, (30.75% identity in 221 aa overlap); and to other integral membrane proteins (principally from Streptomyces sp.) e.g. Q9EWZ8|2SCG38.21 from Streptomyces coelicolor (302 aa), FASTA scores: opt: 365, E(): 5.2e-15, (32.0% identity in 278 aa overlap); Q9S267|SCI30A.06 from Streptomyces coelicolor (297 aa), FASTA scores: opt: 356, E(): 1.8e-14, (31.5% identity in 289 aa overlap); AAK81278|CAC3346 from Clostridium acetobutylicum (472 aa), FASTA scores: opt: 154, E(): 0.038, (24.1% identity in 224 aa overlap); etc. O33234 No cell wall and cell processes O33234 O33234 NC_000962.3 Mycobrowser_v5 CDS 3044375 3044989 . - 0 Rv2732c Rv2732c Unknown Probable conserved transmembrane protein Rv2732c, (MTCY174.12c), len: 204 aa. Probable conserved transmembrane protein, similar to Q49834 hypothetical protein B2235_C1_155 from Mycobacterium leprae (209 aa), FASTA scores: opt: 932, E(): 0, (70.6% identity in 201 aa overlap). Contains PS00343 Gram-positive cocci surface proteins 'anchoring' hexapeptide. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O33237 No cell wall and cell processes O33237 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 3046821 3047675 . + 0 Rv2734 Rv2734 Function unknown Conserved hypothetical protein Rv2734, (MTCY154.14), len: 284 aa. Conserved hypothetical protein, highly similar to various proteins e.g. Q984J2|MLR7981 ABC transporter ATP-binding protein from Rhizobium loti (Mesorhizobium loti) (286 aa), FASTA scores: opt: 877, E(): 9e-50, (52.45% identity in 246 aa overlap) (N-terminus longer); Q98DH1|MLL4707 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (249 aa), FASTA scores: opt: 829, E(): 1.1e-46, (50.4% identity in 244 aa overlap); AAK65865|SMA2239 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (259 aa), FASTA scores: opt: 796, E(): 1.5e-44, (50.0% identity in 252 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6YA42 No conserved hypotheticals O33239 I6YA42 NC_000962.3 Mycobrowser_v5 CDS 3047560 3048552 . - 0 Rv2735c Rv2735c Unknown Conserved hypothetical protein Rv2735c, (MTCY154.15c), len: 330 aa. Conserved hypothetical protein, showing some similarity with Q98DH2|MLR4706 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (302 aa), FASTA scores: opt: 140, E(): 0.062, (27.0% identity in 200 aa overlap); and Q9PHA1|XF0043 hypothetical protein from Xylella fastidiosa (293 aa), FASTA scores: opt: 120, E(): 1.2, (30.75% identity in 117 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6Y1K7 No conserved hypotheticals I6Y1K7 I6Y1K7 NC_000962.3 Mycobrowser_v5 CDS 3110507 3110737 . - 0 Rv2801A mazE9 Unknown Possible antitoxin MazE9 Rv2801A, len: 76 aa. Possible mazE9, antitoxin, part of toxin-antitoxin (TA) operon with Rv2801c (See Pandey and Gerdes, 2005; Zhu et al., 2006). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P0CL61 No virulence, detoxification, adaptation P0CL61 NC_000962.3 Mycobrowser_v5 CDS 3048562 3049086 . - 0 Rv2736c recX May play a regulatory role possibly by interacting with RECA, the product of the upstream ORF. Regulatory protein RecX Rv2736c, (MTV002.01c), len: 174 aa. Probable recX, regulatory protein (see citation below), equivalent to P37859|RECX_MYCLE|ML0988|U2235B regulatory protein RECX from Mycobacterium leprae (171 aa), FASTA scores: opt: 848, E(): 2e-46, (77.0% identity in 174 aa overlap); and CAA67596|RECX|P94965|RECX_MYCSM regulatory protein RECX from Mycobacterium smegmatis (188 aa), FASTA scores: opt: 679, E(): 8.8e-36, (66.45% identity in 164 aa overlap). Also similar (or highly similar to) others e.g. O50488|RECX_STRCO|SC4H8.09 from Streptomyces coelicolor (188 aa), FASTA scores: opt: 371, E(): 1.9e-16, (42.7% identity in 164 aa overlap); Q9LCZ3|RECX from Xanthomonas campestris pv. citri (162 aa), FASTA scores: opt: 189, E(): 4.4e-05, (32.45% identity in 151 aa overlap); P37860|RECX_PSEAE|PA3616 from Pseudomonas aeruginosa (153 aa), FASTA scores: opt: 159, E(): 0.0032, (30.65% identity in 137 aa overlap); etc. Belongs to the RecX family. P9WHI1 No information pathways P0A5U8 P9WHI1 GO:0006282,GO:0005737 P9WHI1 NC_000962.3 Mycobrowser_v5 CDS 3101581 3102162 . - 0 Rv2792c Rv2792c Prevents the cointegration of foreign DNA before integration into the chromosome. Possible resolvase Rv2792c, (MTV002.57c), len: 193 aa. Possible IS1602 resolvase, highly similar to many from Mycobacterium tuberculosis e.g. O07773|Rv0605|MTCY19H5.17c possible resolvase (202 aa), FASTA scores: opt: 1040, E(): 1.9e-62, (85.05% identity in 194 aa overlap). Contains PS00397 Site-specific recombinases active site and possible helix-turn-helix motif at aa 1-2 (Score 1687, +4.93 SD). I6YA93 No insertion seqs and phages O33334 I6YA93 GO:0006310,GO:0000150,GO:0003677 I6YA93 NC_000962.3 Mycobrowser_v5 CDS 3053233 3053682 . + 0 Rv2740 ephG Biotransformation enzyme that catalyzes the hydrolysis of epoxides (alkene oxides, oxiranes) and arene oxides to less reactive and more water soluble dihydrodiols by the trans addition of water. Involved in detoxification reactions following oxidative damage to lipids [catalytic activity: an epoxide + H(2)O = a glycol]. Epoxide hydrolase Rv2740, (MTV002.05), len: 149 aa. EphG, Epoxide hydrolase, proven biochemically (see Unge et al. 2005), similar to limonene-1,2-epoxide hydrolase capable of hydrolyzing long or bulky lipophilic epoxides. Equivalent, but shorter 17 aa, to Q9CCC2|ML0984 (alias Q49850 but longer) hypothetical protein from Mycobacterium leprae (164 aa), FASTA scores: opt: 481, E(): 9.7e-26, (52.0% identity in 150 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O33283 No virulence, detoxification, adaptation O33283 O33283 O33283 NC_000962.3 Mycobrowser_v5 CDS 3053914 3055491 . + 0 Rv2741 PE_PGRS47 Function unknown PE-PGRS family protein PE_PGRS47 Rv2741, (MTV002.06), len: 525 aa. PE_PGRS47, Member of the M. tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to others e.g. Q10637|YD25_MYCTU|Rv1325c|MT1367|MTCY130.10c hypothetical PE-PGRS family protein (603 aa), FASTA scores: opt: 1936, E(): 1.1e-71, (56.95% identity in 611 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q79FB3 No PE/PPE Q79FB3 Q79FB3 Q79FB3 NC_000962.3 Mycobrowser_v5 CDS 3055515 3056348 . - 0 Rv2742c Rv2742c Function unknown Conserved hypothetical arginine rich protein Rv2742c, (MTV002.07c), len: 277 aa (questionable ORF). Conserved hypothetical arg-rich protein. Extreme N-terminus is highly similar to the N-teminus of Q9CCC1ML0986 hypothetical protein from Mycobacterium leprae (67 aa), FASTA scores: opt: 183, E(): 0.00052, (71.05% identity in 38 aa overlap); and to the downstream ORF O33281|Rv2738c|MTV002.03c conserved hypothetical protein from Mycobacterium tuberculosis (68 aa), FASTA scores: opt: 200, E(): 5.5e-05, (78.4% identity in 37 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O33285 No conserved hypotheticals O33285 O33285 NC_000962.3 Mycobrowser_v5 CDS 3058193 3058531 . - 0 Rv2745c clgR Transcriptional regulator Transcriptional regulatory protein ClgR Rv2745c, (MTV002.10c), len: 112 aa. ClgR, transcriptional regulatory protein, controls protease systems and chaperones. P9WMH7 No regulatory proteins O33287 P9WMH7 GO:0043565 P9WMH7 NC_000962.3 Mycobrowser_v5 CDS 3071267 3071536 . - 0 Rv2760c vapB42 Unknown Possible antitoxin VapB42 Rv2760c, (MTV002.25c), len: 89 aa. Possible vapB42, antitoxin, part of toxin-antitoxin (TA) operon with Rv2759c, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. O07770|Rv0608|MTCY19H5.14c (81 aa), FASTA scores: opt: 128, E(): 0.057, (37.5% identity in 88 aa overlap); and P96913|Rv0623|MTCY20H10.04 (84 aa), FASTA scores: opt: 99, E(): 5.5, (37.1% identity in 89 aa overlap). Also showing some similarity with CAC45377|SMC00899 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (84 aa), FASTA scores: opt: 116, E(): 0.38, (36.25% identity in 91 aa overlap). P9WJ19 No virulence, detoxification, adaptation O33302 P9WJ19 NC_000962.3 Mycobrowser_v5 CDS 3081119 3081592 . - 0 Rv2772c Rv2772c Unknown Probable conserved transmembrane protein Rv2772c, (MTV002.37c), len: 157 aa. Probable conserved transmembrane protein, equivalent to Q9CBV7|ML1526 conserved membrane protein from Mycobacterium leprae (160 aa), FASTA scores: opt: 767, E(): 1.5e-43, (76.6% identity in 154 aa overlap); and similar to P46830|YDAB_MYCBO from Mycobacterium bovis (177 aa), FASTA scores: opt: 337, E(): 3.9e-15, (40.75% identity in 135 aa overlap). Also similar to O86837|SC9A10.04 putative membrane protein from Streptomyces coelicolor (151 aa), FASTA scores: opt: 338, E(): 3e-15, (43.75% identity in 144 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). I6YA75 No cell wall and cell processes I6YA75 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 3059262 3059786 . + 0 Rv2747 argA Involved in arginine biosynthesis [catalytic activity: L-glutamate + acetyl-CoA <=> N-acetyl-L-glutamate + coenzyme A] Probable L-glutamate alpha-N-acetyltranferase ArgA (alpha-N-acetylglutamate synthase) Rv2747, (MTV002.12), len: 174 aa. Probable argA, alpha-N-acetylglutamate synthase (See Errey et al., 2005). Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Equivalent to O05559|ML0978|MLCB33.08 putative acetyltransferase from Mycobacterium leprae (180 aa), FASTA scores: opt: 997, E(): 1.2e-57, (86.8% identity in 174 aa overlap). Also similar to various transferases e.g. Q9X8N2|SCE94.27c putative acetyltransferase from Streptomyces coelicolor (169 aa), FASTA scores: opt: 656, E(): 1.3e-35, (60.35% identity in 164 aa overlap); C-terminus of Q9K3D6|ARGH(A) argininosuccinase and N-acetylglutamate synthase from Moritella sp. 2693 (629 aa), FASTA scores: opt: 243, E(): 2e-08, (31.95% identity in 144 aa overlap); C-terminus of Q9JW21|ARGA or NMA0580 putative acetylglutamate synthase from Neisseria meningitidis serogroup a (436 aa), FASTA scores: opt: 201, E(): 7.8e-06, (32.75% identity in 119 aa overlap); etc. Also similar to hypothetical proteins e.g. O67372|AQ_1359 hypothetical 21.1 KDA protein from Aquifex aeolicus (181 aa), FASTA scores: opt: 348, E(): 1.2e-15, (42.35% identity in 137 aa overlap). O33289 No intermediary metabolism and respiration O33289 O33289 GO:0008152,GO:0008080 2.-.-.- O33289 NC_000962.3 Mycobrowser_v5 CDS 3059855 3062506 . - 0 Rv2748c ftsK Possibly involved in cell division processes Possible cell division transmembrane protein FtsK Rv2748c, (MTV002.13c), len: 883 aa. Possible ftsK, cell division transmembrane protein, equivalent to O05560|ML0977|FTSK|MLCB33.09c cell division protein from Mycobacterium leprae (886 aa), FASTA scores: opt: 3147, E(): 7.9e-175, (78.1% identity in 885 aa overlap). Also similar to other members of the spoIIIE/ftsK family e.g. O86810|SC7C7.05 FTSK homolog from Streptomyces coelicolor (929 aa), FASTA scores: opt: 2256, E(): 3.8e-123, (49.05% identity in 924 aa overlap); Q9CF25|FTSK cell division protein FTSK from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (763 aa), FASTA scores: opt: 1438, E(): 9.1e-76, (37.7% identity in 751 aa overlap); AAK75005|Q97RE4|SP0878 SPOE family protein from Streptococcus pneumoniae (767 aa), FASTA scores: opt: 1405, E(): 7.5e-74, (48.0% identity in 477 aa overlap); P46889|FTSK_ECOLI|B0890 from Escherichia coli strain K12 (1329 aa), FASTA scores: opt: 759, E(): 0, (44.5% identity in 537 aa overlap) (similarity in C-terminal half); etc. Equivalent to AAK47139 from Mycobacterium tuberculosis strain CDC1551 (968 aa) but shorter 85 aa. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the FTSK/SPOIIIE family. P9WNA3 No cell wall and cell processes O33290 P9WNA3 GO:0003677,GO:0007049,GO:0051301,GO:0007059,GO:0016021,GO:0017111,GO:0005886,GO:0005524 P9WNA3 NC_000962.3 Mycobrowser_v5 CDS 3082909 3083370 . + 0 Rv2775 Rv2775 Acetylation, substrate unknown GCN5-related N-acetyltransferase Rv2775, (MTV002.40), len: 153 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Showing weak similarity to other hypothetical proteins e.g. Q9ZBJ7|SC9C7.13c from Streptomyces coelicolor (179 aa), FASTA scores: opt: 167, E(): 0.00024, (29.05% identity in 148 aa overlap). Equivalent to AAK47164 from Mycobacterium tuberculosis strain CDC1551 (185 aa) but shorter 32 aa. O33317 No intermediary metabolism and respiration O33317 O33317 GO:0008152,GO:0008080 O33317 NC_000962.3 Mycobrowser_v5 CDS 3085713 3086183 . - 0 Rv2778c Rv2778c Function unknown Conserved protein Rv2778c, (MTV002.43c), len: 156 aa. Conserved protein, similar to Q9CBF7|ML2031 hypothetical protein from Mycobacterium leprae (151 aa), FASTA scores: opt: 227, E(): 8.5e-09, (35.95% identity in 153 aa overlap). Also similar to AAK46204|MT1931.1 hypothetical 17.8 KDA protein from Mycobacterium tuberculosis strain CDC1551 (158 aa), FASTA scores: opt: 238, E(): 1.5e-09, (35.75% identity in 151 aa overlap); or O07748|Rv1883c|MTCY180.35 hypothetical 17.3 KDA protein from Mycobacterium tuberculosis strain H37Rv (158 aa), FASTA scores: opt: 212, E(): 9.7e-08, (34.45% identity in 151 aa overlap); note that AAK46204|MT1931.1 and O07748|Rv1883c|MTCY180.35 are essentially the same protein except for a small (5 aa) gap. I6Y1P2 No conserved hypotheticals O33320 I6Y1P2 I6Y1P2 NC_000962.3 Mycobrowser_v5 CDS 3063638 3064528 . + 0 Rv2751 Rv2751 Function unknown Conserved protein Rv2751, (MTV002.16), len: 296 aa. Conserved protein, similar in part to others e.g. Q98LR1|MLR0915 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (299 aa), FASTA scores: opt: 279, E(): 1.6e-11, (32.85% identity in 210 aa overlap); Q9FBX1|SC8E7.10 conserved hypothetical protein from Streptomyces coelicolor (283 aa), FASTA scores: opt: 232, E(): 2.4e-08, (27.9% identity in 269 aa overlap); Q9FMY9 hypothetical protein (genomic DNA, chromosome 5, P1 clone:MJB21) from Arabidopsis thaliana (Mouse-ear cress) (370 aa), FASTA scores: opt: 205, E(): 2.1e-06, (28.9% identity in 211 aa overlap); etc. Also similar in part to several proteins from Mycobacterium tuberculosis: P72053|Rv3787c|MTCY13D12.21 hypothetical 33.4 KDA protein (308 aa), FASTA scores: opt: 266, E(): 1.3e-10, (29.6% identity in 267 aa overlap); O53795|MBE50c|Rv0731c|MTV041.05c hypothetical 34.9 KDA protein (318 aa), FASTA scores: opt: 266, E(): 1.3e-10, (32.05% identity in 281 aa overlap); O53841|Rv0830|MTV043.22 hypothetical 33.4 KDA protein (301 aa), FASTA scores: opt: 263, E(): 2e-10, (31.3% identity in 262 aa overlap); etc. Belongs to the MTCY13D12.21 / MTCY210.45C / MTCY78.29C family. I6YEA3 No conserved hypotheticals O33293 I6YEA3 GO:0008168 I6YEA3 NC_000962.3 Mycobrowser_v5 CDS 3064515 3066191 . - 0 Rv2752c Rv2752c Function unknown Conserved hypothetical protein Rv2752c, (MTV002.17c), len: 558 aa. Conserved hypothetical protein, equivalent to Q9CBW5|ML1512 hypothetical protein from Mycobacterium leprae (558 aa), FASTA scores: opt: 3301, E(): 1.2e-195, (89.05% identity in 558 aa overlap). Also highly similar to other hypothetical proteins from a wide range of prokaryotes e.g. CAC19480|P54122|YOR4_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (718 aa), FASTA scores: opt: 2142, E(): 3.5e-124, (57.2% identity in 554 aa overlap) (N-terminus longer); O86842|SC9A10.09 from Streptomyces coelicolor (561 aa), FASTA scores: opt: 2077, E(): 2.9e-120, (55.95% identity in 556 aa overlap); Q9ZI80 from Streptomyces toyocaensis (528 aa), FASTA scores: opt: 1843, E(): 7.3e-106, (52.45% identity in 528 aa overlap) (N-terminus shorter 30 aa); etc. P9WGZ9 No conserved hypotheticals O33294 P9WGZ9 GO:0016787 P9WGZ9 NC_000962.3 Mycobrowser_v5 CDS 3066222 3067124 . - 0 Rv2753c dapA Involved in biosynthesis of diaminopimelate and lysine from aspartate semialdehyde (at the first step) [catalytic activity: L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H(2)O]. Probable dihydrodipicolinate synthase DapA (DHDPS) (dihydrodipicolinate synthetase) Rv2753c, (MT2823, MTV002.18c), len: 300 aa. Probable dapA, dihydrodipicolinate synthase, equivalent to Q9CBW4|DAPA_MYCLE|ML1513 dihydrodipicolinate synthase from Mycobacterium leprae (300 aa), FASTA scores: opt: 1699, E(): 2.2e-98, (86.65% identity in 300 aa overlap). Also highly similar to many e.g. P19808|DAPA_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (301 aa), FASTA scores: opt: 1089, E(): 2e-60, (58.7% identity in 288 aa overlap); O86841|DAPA_STRCO|SC9A10.08 from Streptomyces coelicolor (299 aa), FASTA scores: opt: 1044, E(): 1.3e-57, (55.75% identity in 287 aa overlap); P05640|DAPA_ECOLI (292 aa), FASTA scores: opt: 515, E(): 0, (33.8% identity in 287 aa overlap); etc. Contains PS00665 and PS00666 Dihydrodipicolinate synthetase signatures 1 and 2. Belongs to the DHDPS family. P9WP25 No intermediary metabolism and respiration 1XXX,3L21 P63945 P9WP25 GO:0019877,GO:0008840,GO:0005737 4.2.1.52 P9WP25 NC_000962.3 Mycobrowser_v5 CDS 3072637 3073056 . - 0 Rv2762c Rv2762c Function unknown Conserved hypothetical protein Rv2762c, (MTV002.27c), len: 139 aa. Conserved hypothetical protein, similar to C-terminus of hypothetical proteins: Q9A380|CC3324 from Caulobacter crescentus (409 aa), FASTA scores: opt: 181, E(): 9.8e-05, (43.55% identity in 101 aa overlap); Q98KQ4|MLR1373 from Rhizobium loti (Mesorhizobium loti) (399 aa), FASTA scores: opt: 174, E(): 0.00028, (46.35% identity in 82 aa overlap); and Q9HZZ9|PA2844 from Pseudomonas aeruginosa (402 aa), FASTA scores: opt: 158, E(): 0.0033, (40.0% identity in 80 aa overlap). I6X5B0 No conserved hypotheticals O33304 I6X5B0 NC_000962.3 Mycobrowser_v5 CDS 3107311 3107637 . - 0 Rv2798c Rv2798c Function unknown Conserved hypothetical protein Rv2798c, (MTCY16B7.45), len: 108 aa. Conserved hypothetical ala-rich protein, similar to P71545|Y965_MYCTU|Rv0965c|MT0993|MTCY10D7.09 hypothetical 14.5 KDA protein from Mycobacterium tuberculosis (139 aa), FASTA scores: opt: 198, E(): 8e-07, (38.9% identity in 90 aa overlap). P71653 No conserved hypotheticals P71653 NC_000962.3 Mycobrowser_v5 CDS 3067193 3067945 . - 0 Rv2754c thyX Catalyzes the formation of dTMP and tetrahydrofolate from dUMP and methylenetetrahydrofolate Probable thymidylate synthase ThyX (ts) (TSase) Rv2754c, (MTV002.19c), len: 250 aa. Probable thyX, thymidylate synthase, highly similar to Q9CBW3|YF14_MYCLE|ML1514 thymidylate synthase from Mycobacterium leprae (254 aa), FASTA scores: opt: 1351, E(): 1e-84, (81.5% identity in 254 aa overlap). Also highly similar to several others e.g P40111|THYX_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (250 aa), FASTA scores: opt: 1080, E(): 9.8e-67, (62.85% identity in 245 aa overlap); Q05259|THYX_BPML5 Probable thymidylate synthase from Mycobacteriophage L5 (243 aa), FASTA scores: opt: 610, E(): 3.2e-34, (49.55% identity in 220 aa overlap); etc. Contains Pfam match to entry PF02511 Thymidylate synthase complementing protein. Belongs to the THY1 family. P9WG57 No intermediary metabolism and respiration 2AF6,2GQ2,3GWC,3HZG P66930 P9WG57 GO:0006231,GO:0050797,GO:0050660 2.1.1.148 P9WG57 NC_000962.3 Mycobrowser_v5 CDS 3070170 3070586 . - 0 Rv2757c vapC21 Unknown Possible toxin VapC21 Rv2757c, (MTV002.22c), len: 138 aa. Possible vapC21, toxin, part of toxin-antitoxin (TA) operon with Rv2758c, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to several others in M. tuberculosis e.g. P96411|Rv0229c| MTCY08D5.24c (226 aa), FASTA scores: opt: 354, E(): 4.6e-18, (45.25% identity in 137 aa overlap) (N-terminus longer 89 aa); P95007|RV2546|MTCY159.10c (137 aa), FASTA scores: opt: 265, E(): 7.5e-12, (38.5% identity in 135 aa overlap); O07228|Rv0301|MTCY63.06 (141 aa), FASTA scores: opt: 259, E(): 2.1e-11, (42.4% identity in 132 aa overlap); etc. P9WF91 No virulence, detoxification, adaptation O33299 P9WF91 P9WF91 NC_000962.3 Mycobrowser_v5 CDS 3070583 3070849 . - 0 Rv2758c vapB21 Unknown Possible antitoxin VapB21 Rv2758c, (MTV002.23c), len: 88 aa. Possible vapB21, antitoxin, part of toxin-antitoxin (TA) operon with Rv2757c (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to several others in M. tuberculosis e.g. P95008|Rv2545 (92 aa), FASTA scores: opt: 151, E(): 0.00028, (66.65% identity in 45 aa overlap); Q10771|YF60_MYCTU|RV1560|MT1611|MTCY48.05c (72 aa), FASTA scores: opt: 106, E(): 0.52, (39.15% identity in 46 aa overlap); O06565|Rv1113|MTCY22G8.02 (65 aa), FASTA scores: opt: 97, E(): 2.2, (33.35% identity in 69 aa overlap); etc. Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature. P9WJ43 No virulence, detoxification, adaptation O33300 P9WJ43 NC_000962.3 Mycobrowser_v5 CDS 3070875 3071270 . - 0 Rv2759c vapC42 Unknown Possible toxin VapC42. Contains PIN domain. Rv2759c, (MTV002.24c), len: 131 aa. Possible vapC42, toxin, part of toxin-antitoxin (TA) operon with Rv2760c, contains PIN domain, see Arcus et al. 2005. Similar to others in M. tuberculosis e.g. O07769|Y609_MYCTU|Rv0609|MT0638|MTCY19H5.13c (133 aa), FASTA scores: opt: 364, E(): 5.1e-18, (49.6% identity in 131 aa overlap); P96914|Y624_MYCTU|Rv0624|MT0652|MTCY20H10.05 (131 aa), FASTA scores: opt: 324, E(): 2.9e-15, (42.85% identity in 126 aa overlap); and Q10874|YJ82_MYCTU|Rv1982c|MT2034|MTCY39.37 (139 aa), FASTA scores: opt: 271, E(): 1.4e-11, (38.6% identity in 127 aa overlap). Also similar to other hypothetical proteins from other bacteria e.g. CAC45376|SMC00900 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (128 aa), FASTA scores: opt: 286, E(): 1.2e-12, (39.55% identity in 129 aa overlap); Q981I7|MLL9357 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (131 aa), FASTA scores: opt: 257, E(): 1.2e-10, (36.35% identity in 132 aa overlap); Q9AAG1|CC0639 hypothetical protein from Caulobacter crescentus (131 aa), FASTA scores: opt: 217, E(): 6.9e-08, (33.35% identity in 132 aa overlap); etc. P9WF57 No virulence, detoxification, adaptation P67242 P9WF57 P9WF57 NC_000962.3 Mycobrowser_v5 CDS 3073130 3073609 . - 0 Rv2763c dfrA Essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP [catalytic activity: 5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH]. Dihydrofolate reductase DfrA (DHFR) (tetrahydrofolate dehydrogenase) Rv2763c, (MTV002.28c), len: 159 aa. Probable dfrA (alternate gene names: folA, dhfr), dihydrofolate reductase, equivalent to O30463|FOLA dihydrofolate reductase from Mycobacterium avium (see citation below) (181 aa), FASTA scores: opt: 802, E(): 4.5e-48, (70.2% identity in 161 aa overlap); and Q9CBW1|FOLA|ML1518 dihydrofolate reductase from Mycobacterium leprae (165 aa), FASTA scores: opt: 782, E(): 1e-46, (70.55% identity in 163 aa overlap). Also highly similar to many e.g. Q9K168|DYR_NEIMB|FOLA|NMB0308 from Neisseria meningitidis (serogroup B) (162 aa), FASTA scores: opt: 469, E(): 3.8e-25, (46.65% identity in 163 aa overlap); P12833|DYR3_SALTY|DHFRIII from Salmonella typhimurium (162 aa), FASTA scores: opt: 367, E(): 4e-18, (45.4% identity in 141 aa overlap); Q59408|DYRC_ECOLI|DHFRXIII from Escherichia coli strain RA33.2 (165 aa), FASTA scores: opt: 313, E(): 2.2e-14, (41.9% identity in 136 aa overlap); etc. Contains PS00075 Dihydrofolate reductase signature. Belongs to the dihydrofolate reductase family. P9WNX1 No intermediary metabolism and respiration 1DF7,1DG5,1DG7,1DG8,2CIG P0A546 P9WNX1 GO:0004146,GO:0006545,GO:0009165,GO:0006730,GO:0055114,GO:0050661 1.5.1.3 P9WNX1 NC_000962.3 Mycobrowser_v5 CDS 3073680 3074471 . - 0 Rv2764c thyA Involved in deoxyribonucleotide biosynthesis. Provides the sole de novo source of dTMP for DANA biosynthesis [catalytic activity: 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP]. Probable thymidylate synthase ThyA (ts) (TSASE) Rv2764c, (MTV002.29c), len: 263 aa. Probable thyA, thymidylate synthase, equivalent to Q9CBW0|TYSY_MYCLE|THYA|ML1519 thymidylate synthase from Mycobacterium leprae (266 aa), FASTA scores: opt: 1602, E(): 5.9e-102, (85.5% identity in 262 aa overlap). Also highly similar to many e.g. P00470|TYSY_ECOLI|B2827|Z4144|ECS3684|BAB37107|AAG57938 from Escherichia coli strains K12 and O157:H7 (264 aa), FASTA scores: opt: 1309, E(): 5.9e-82, (66.65% identity in 261 aa overlap); P48464|TYSY_SHIFL|THYA from Shigella flexneri (264 aa), FASTA scores: opt: 1303, E(): 1.5e-81, (65.9% identity in 261 aa overlap); P54081|TYSB_BACAM|THYB|THYBA from Bacillus amyloliquefaciens (264 aa), FASTA scores: opt: 1235, E(): 6.7e-77, (66.65% identity in 261 aa overlap); etc. Contains PS00091 Thymidylate synthase active site. Belongs to the thymidylate synthase family. P9WFR9 No intermediary metabolism and respiration 3QJ7 P67044 P9WFR9 GO:0006231,GO:0004799,GO:0005737 2.1.1.45 P9WFR9 NC_000962.3 Mycobrowser_v5 CDS 3075588 3076370 . - 0 Rv2766c Rv2766c Function unknown, possibly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv2766c, (MTV002.31c), len: 260 aa. Probable short-chain dehydrogenase/reductase , similar to others (from bacteria and eukaryota) e.g. Q9K3Y8|2SCG61.27c putative short chain oxidoreductase from Streptomyces coelicolor (253 aa), FASTA scores: opt: 722, E(): 7.4e-39, (44.75% identity in 248 aa overlap); Q93790|F54F3.4 hypothetical SDR protein from Caenorhabditis elegans (260 aa), FASTA scores: opt: 613, E(): 6.9e-32, (41.7% identity in 247 aa overlap); O95162|O95162|scad-SRL peroxisomal short-chain alcohol dehydrogenase from Homo sapiens (Human) (260 aa), FASTA scores: opt: 594, E(): 1.1e-30, (39.6% identity in 250 aa overlap); P51831|FABG_BACSU 3-oxoacyl-[acyl-carrier protein] from Bacillus subtilis (246 aa), FASTA scores: opt: 504, E(): 4e-28, (37.2% identity in 247 aa overlap); etc. Also similar to many other Mycobacterium tuberculosis acyl-carrier proteins e.g. MTCY03C7.07 (38.5% identity in 244 aa overlap). Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. Note that previously known as fabG5, a 3-oxoacyl-[acyl-carrier-protein]. I6YEB6 No intermediary metabolism and respiration Q7D6M3 I6YEB6 GO:0055114,GO:0016491 1.-.-.- I6YEB6 NC_000962.3 Mycobrowser_v5 CDS 3134596 3135483 . - 0 Rv2827c Rv2827c Unknown Hypothetical protein Rv2827c, (MTCY16B7.15), len: 295 aa. Hypothetical unknown protein, equivalent to AAK47219 from Mycobacterium tuberculosis strain CDC1551 (315 aa) but shorter 20 aa. P71625 No conserved hypotheticals 1ZEL P71625 P71625 P71625 NC_000962.3 Mycobrowser_v5 CDS 3164152 3165192 . + 0 Rv2854 Rv2854 Unknown Unknown protein Rv2854, (MTCY24A1.03c), len: 346 aa. Unknown protein, showing similarity with Q9CD03|ML2603 hypothetical protein from Mycobacterium leprae (279 aa), FASTA scores: opt: 154, E(): 0.0083, (33.35% identity in 87 aa overlap). O05805 No conserved hypotheticals O05805 NC_000962.3 Mycobrowser_v5 CDS 3076367 3076720 . - 0 Rv2767c Rv2767c Unknown Possible membrane protein Rv2767c, (MTV002.32c), len: 117 aa (questionable ORF). Possible membrane protein, showing very weak similarity with Q9L2H7|SCC121.09 putative metal transport ABC transporter from Streptomyces coelicolor (256 aa), FASTA scores: opt: 110, E(): 1, (33.05% identity in 112 aa overlap). O33309 No cell wall and cell processes O33309 NC_000962.3 Mycobrowser_v5 CDS 3080581 3081033 . - 0 Rv2771c Rv2771c Function unknown Conserved hypothetical protein Rv2771c, (MTV002.36c), len: 150 aa. Conserved hypothetical protein, equivalent to Q9CBV8|ML1525 hypothetical protein from Mycobacterium leprae (151 aa), FASTA scores: opt: 489, E(): 1.7e-27, (52.7% identity in 148 aa overlap). Also highly similar to Q9RD46|SCF56.21 hypothetical 15.7 KDA protein from Streptomyces coelicolor (151 aa), FASTA scores: opt: 671, E(): 2.2e-40, (67.8% identity in 146 aa overlap). O33313 No conserved hypotheticals O33313 O33313 O33313 NC_000962.3 Mycobrowser_v5 CDS 3178458 3179312 . - 0 Rv2867c Rv2867c Acetylation, substrate unknown GCN5-related N-acetyltransferase Rv2867c, (MTV003.13c), len: 284 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain in C-terminal part. See Vetting et al. 2005. Similar to others e.g. Q9KYR8|SC5H4.21 hypothetical 31.3 KDA protein from Streptomyces coelicolor (287 aa), FASTA scores: opt: 798, E(): 2.4e-45, (47.95% identity in 269 aa overlap). I6XFI7 No intermediary metabolism and respiration O33349 I6XFI7 GO:0008152,GO:0008080 I6XFI7 NC_000962.3 Mycobrowser_v5 CDS 3084485 3085555 . - 0 Rv2777c Rv2777c Function unknown Conserved hypothetical protein Rv2777c, (MTV002.42c), len: 356 aa. Conserved hypothetical protein, highly similar (but longer in N-terminus) to hypothetical proteins Q9KZ16|SC10B7.16 from Streptomyces coelicolor (296 aa), FASTA scores: opt: 980, E(): 6.8e-57, (51.25% identity in 281 aa overlap); and Q9HYS0|PA3325 from Pseudomonas aeruginosa (295 aa), FASTA scores: opt: 816, E(): 4e-46, (43.75% identity in 288 aa overlap); and similar (but longer in N-terminus) to other hypothetical proteins e.g. Q9I3H1|PA1542 from Pseudomonas aeruginosa (278 aa), FASTA scores: opt: 234, E(): 6.3e-08, (31.8% identity in 258 aa overlap). Equivalent to AAK47166 from Mycobacterium tuberculosis strain CDC1551 (393 aa) but shorter 37 aa. O33319 No conserved hypotheticals O33319 O33319 O33319 NC_000962.3 Mycobrowser_v5 CDS 3086215 3086754 . - 0 Rv2779c Rv2779c Involved in transcriptional mechanism. Possible transcriptional regulatory protein (probably Lrp/AsnC-family) Rv2779c, (MTV002.44c), len: 179 aa. Possible transcriptional regulator, from the Lrp/AsnC family, similar (but longer ~30 aa in N-terminus) to others e.g. CAC42842|SCBAC36F5.06 putative AsnC-family transcriptional regulatory protein from Streptomyces coelicolor (163 aa), FASTA scores: opt: 333, E(): 4.4e-16, (39.7% identity in 141 aa overlap); O07920|AZLB_BACSU transcriptional regulator (AsnC family) from Bacillus subtilis; Q9I233|PA2082 probable transcriptional regulator (AsnC family) from Pseudomonas aeruginosa (158 aa), FASTA scores: opt: 322, E(): 2.5e-15, (33.1% identity in 148 aa overlap); etc. Also similar to P96896|Rv3291c|MTCY71.31c from Mycobacterium tuberculosis (33.3% identity in 120 aa overlap). Equivalent to AAK47168 from Mycobacterium tuberculosis strain CDC1551 (181 aa). Seems to belong to the AsnC family of transcriptional regulators. Start changed since first submission (+8 aa). O33321 No regulatory proteins O33321 O33321 GO:0006355,GO:0043565,GO:0006350,GO:0003700,GO:0005622 O33321 NC_000962.3 Mycobrowser_v5 CDS 3087950 3088984 . - 0 Rv2781c Rv2781c Function unknown; probably involved in cellular metabolism. Possible alanine rich oxidoreductase Rv2781c, (MTV002.46c), len: 344 aa. Possible ala-rich oxidoreductase, similar to various oxidoreductases or hypothetical proteins e.g. Q9RDD8|SCC77.20c putative oxidoreductase from Streptomyces coelicolor (364 aa), FASTA scores: opt: 912, E(): 5.3e-47, (45.55% identity in 336 aa overlap); Q9FDD4|2-NPDL putative 2-nitropropane dioxygenase from Streptomyces ansochromogenes (363 aa), FASTA scores: opt: 869, E(): 1.9e-44, (44.2% identity in 337 aa overlap); O05413|YRPB 2-nitropropane dioxygenase from Bacillus subtilis (347 aa), FASTA scores: opt: 560, E(): 4.9e-26, (33.75% identity in 317 aa overlap); etc. I6X5C5 No intermediary metabolism and respiration O33323 I6X5C5 GO:0055114,GO:0018580 1.-.-.- I6X5C5 NC_000962.3 Mycobrowser_v5 CDS 3135788 3136333 . - 0 Rv2828c Rv2828c Function unknown Conserved hypothetical protein Rv2828c, (MTCY16B7.14), len: 181 aa. Conserved hypothetical protein, similar to Q9RY53|DR0097 conserved hypothetical protein from Deinococcus radiodurans (189 aa), FASTA scores: opt: 267, E(): 1.9e-11, (34.1% identity in 176 aa overlap); and shows some similarity with N-terminus of O27278|MTH1210 MRR restriction system related protein from Methanothermobacter thermautotrophicus (340 aa), FASTA scores: opt: 133, E(): 0.07, (28.55% identity in 112 aa overlap). Also equivalent to downstream ORF P71627|Rv2825c|MTCY16B7.17 from Mycobacterium tuberculosis strain H37Rv (alias AAK47217 from strain CDC1551, 246 aa) (215 aa), FASTA scores: opt: 1173, E(): 8.3e-75, (98.9% identity in 181 aa overlap). I6X5G8 No conserved hypotheticals P71624 I6X5G8 NC_000962.3 Mycobrowser_v5 CDS 3090339 3092597 . - 0 Rv2783c gpsI Involved in mRNA degradation. Hydrolyses single-stranded polyribonucleotides processively in the 3' to 5' direction. Involved in the RNA degradosome, a multi-enzyme complex important in RNA processing and messenger RNA degradation [catalytic activity: RNA(N+1) + phosphate = RNA(N) + a nucleoside diphosphate]. Bifunctional protein polyribonucleotide nucleotidyltransferase GpsI: guanosine pentaphosphate synthetase + polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase) (pnpase) Rv2783c, (MTV002.48c), len: 752 aa. Probable gpsI, polyribonucleotide nucleotidyltransferase, equivalent to Q9CCF8|GPSI|ML0854 (alias O32966) putative polyribonucleotide phosphorylase / guanosine pentaphosphate synthetase from Mycobacterium leprae (773 aa), FASTA scores: opt: 4304, E(): 0, (89.95% identity in 757 aa overlap). Also highly similar to others e.g. O86656|GPSI guanosine pentaphosphate synthetase/ polyribonucleotide nucleotidyltransferase (fragment) from Streptomyces coelicolor (716 aa), FASTA scores: opt: 3393, E(): 5.8e-192, (72.77% identity in 718 aa overlap); Q53597|GPSI guanosine pentaphosphate synthetase from Streptomyces antibioticus (740 aa), FASTA scores: opt: 3314, E(): 2.6e-187, (70.55% identity in 733 aa overlap); P72659|PNP|SLL1043 polyribonucleotide nucleotidyltransferase from Synechocystis sp. strain PCC 6803 (718 aa), FASTA scores: opt: 1244, E(): 1.7e-65, (45.05% identity in 750 aa overlap); etc. Note that S. antibioticus guanosine pentaphosphate synthetase is a multifunctional enzyme that also acts as a polyribonucleotide nucleotidyltransferase. Start site chosen by homology from several alternatives. P9WI57 No information pathways O33325 P9WI57 GO:0003723,GO:0006396,GO:0006402,GO:0005739,GO:0004654,GO:0000175 2.7.7.8 P9WI57 NC_000962.3 Mycobrowser_v5 CDS 3092951 3093466 . - 0 Rv2784c lppU Unknown Probable lipoprotein LppU Rv2784c, (MTV002.49c), len: 171 aa. Probable lppU, lipoprotein, sharing no homology with other proteins. Contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. I6XFA6 No cell wall and cell processes I6XFA6 NC_000962.3 Mycobrowser_v5 CDS 3093479 3093748 . - 0 Rv2785c rpsO Involved in translation mechanism. This protein is one of the 16S ribosomal RNA binding proteins. 30S ribosomal protein S15 RpsO Rv2785c, (MTV002.50c), len: 89 aa. rpsO, 30s ribosomal protein S15, equivalent to O32967|RS15_MYCLE|RPSO|ML0853|MLCB22.28c 30S ribosomal protein S15 from Mycobacterium leprae (89 aa), FASTA scores: opt: 522, E(): 7.4e-34, (92.15% identity in 89 aa overlap). Also highly similar to many e.g. O86655|RS15_STRCO|RPSO|SC3C3.22 from Streptomyces coelicolor (95 aa), FASTA scores: opt: 408, E(): 6.7e-25, (62.9% identity in 89 aa overlap); P05766|RS15_BACST|RPSO from Bacillus stearothermophilus (88 aa), FASTA scores: opt: 385, E(): 4e-23, (62.5% identity in 88 aa overlap); P21473|RS15_BACSU|RPSO from Bacillus subtilis (88 aa), FASTA scores: opt: 351, E(): 1.9e-20, (57.95% identity in 88 aa overlap); P02371|RS15_ECOLI|RPSO|sec|B3165 from Escherichia coli strain K12 (88 aa), FASTA scores: opt: 295, E(): 4.5e-22, (52.3% identity in 88 aa overlap); etc. Contains PS00362 Ribosomal protein S15 signature. Belongs to the S15P family of ribosomal proteins. P9WH55 No information pathways P66429 P9WH55 GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WH55 NC_000962.3 Mycobrowser_v5 CDS 3102364 3103260 . - 0 Rv2793c truB Formation of pseudouridine at position 55 in the psi GC loop of transfer RNAS [catalytic activity: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H(2)O]. Probable tRNA pseudouridine synthase B TruB (tRNA pseudouridine 55 synthase) (PSI55 synthase) (pseudouridylate synthase) (uracil hydrolyase) Rv2793c, (MTV002.58c), len: 298 aa. Probable truB, tRNA pseudouridine synthase, equivalent to Q9Z5I4|TRUB_MYCLE|ML1546 or MLCB596.24 tRNA pseudouridine synthase B from Mycobacterium leprae (320 aa), FASTA scores: opt: 1403, E(): 2.9e-83, (74.05% identity in 293 aa overlap). Also highly similar to many e.g. Q9Z528|TRUB_STRCO|SC9F2.07c from Streptomyces coelicolor (301 aa), FASTA scores: opt: 870, E(): 7.6e-49, (50.7% identity in 296 aa overlap); P09171|TRUB_ECOLI|P35|B3166|Z4527|ECS4047 from Escherichia coli strains K12 and O157:H7 (314 aa), FASTA scores: opt: 574, E(): 1e-29, (42.5% identity in 214 aa overlap); Q9PGR1|TRUB_XYLFA|XF0237 from Xylella fastidiosa (302 aa), FASTA scores: opt: 569, E(): 2.1e-29, (41.05% identity in 285 aa overlap); etc. Belongs to the TruB family of pseudouridine synthases. P9WHP7 No information pathways 1SGV P62190 P9WHP7 GO:0009982,GO:0031119,GO:0003723 5.4.99.- P9WHP7 NC_000962.3 Mycobrowser_v5 CDS 3147959 3148258 . - 0 Rv2840c Rv2840c Function unknown Conserved hypothetical protein Rv2840c, (MTCY16B7.02), len: 99 aa. Conserved hypothetical protein, equivalent to Q9Z5J0|ML1557|MLCB596.13 hypothetical 11.6 KDA protein from Mycobacterium leprae (106 aa), FASTA scores: opt: 501, E(): 2.3e-29, (501% identity in 96 aa overlap). Also highly similar to other hypothetical proteins e.g. Q9KYR0|SC5H4.29 from Streptomyces coelicolor (101 aa), FASTA scores: opt: 256, E(): 1.4e-11, (50.6% identity in 81 aa overlap); Q9APM9 from Myxococcus xanthus (111 aa), FASTA scores: opt: 174, E(): 1.3e-05, (42.25% identity in 97 aa overlap); and similar to to others e.g. N-terminus of CAC41675|SMC02913 from Rhizobium meliloti (Sinorhizobium meliloti) (230 aa), FASTA scores: opt: 172, E(): 3e-05, (42.4% identity in 66 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). I6XFF7 No conserved hypotheticals P71612 I6XFF7 NC_000962.3 Mycobrowser_v5 CDS 3095111 3096874 . + 0 Rv2787 Rv2787 Function unknown Conserved hypothetical alanine rich protein Rv2787, (MTV002.52), len: 587 aa. Conserved hypothetical ala-rich protein, equivalent to Q9CCI1|ML0798 hypothetical protein from Mycobacterium leprae (592 aa), FASTA scores: opt: 2994, E(): 6.9e-179, (76.5% identity in 587 aa overlap); and similar in part to other proteins from Mycobacterium leprae e.g. O33082|MLCB628.11 hypothetical 52.0 KDA protein (478 aa), FASTA scores: opt: 481, E(): 2.3e-22, (30.95% identity in 294 aa overlap). Also similar in part to O86637|SC3C3.03c hypothetical 112.1 KDA protein from Streptomyces coelicolor (1083 aa), FASTA scores: opt: 488, E(): 1.5e-22, (28.95% identity in 297 aa overlap). And similar to other hypothetical proteins from Mycobacterium tuberculosis e.g. O06396|Rv0530|MTCY25D10.09 (405 aa), FASTA scores: opt: 625, E(): 2.2e-31, (34.05% identity in 320 aa overlap); O69740|Rv3876|MTV027.11 (666 aa), FASTA scores: opt: 453, E(): 1.6e-20, (29.2% identity in 370 aa overlap); P96217|Rv3860|MTCY01A6.08c (390 aa), FASTA scores: opt: 443, E(): 4.7e-20, (29.95% identity in 354 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). O33329 No conserved hypotheticals O33329 O33329 O33329 NC_000962.3 Mycobrowser_v5 CDS 3096959 3097645 . + 0 Rv2788 sirR Involved in transcriptional mechanism. Probable transcriptional repressor SirR Rv2788, (MTV002.53), len: 228 aa. Probable sirR, transcriptional repressor, highly similar to others e.g. Q9RRF3|DR2539 putative iron dependent repressor from Deinococcus radiodurans (232 aa), FASTA scores: opt: 518, E(): 4.5e-26, (41.2% identity in 221 aa overlap); Q9HRU8|SIRR|VNG0536G from Halobacterium sp. strain NRC-1 (233 aa), FASTA scores: opt: 516, E(): 6.1e-26, (40.45% identity in 220 aa overlap); Q9KIJ2|SLOR regulator SLOR from Streptococcus mutans (217 aa), FASTA scores: opt: 418, E(): 1.2e-19, (36.15% identity in 213 aa overlap); etc. Also some similarity to Q50495|IDER_MYCTU|MTCY05A6.32|IDER|DTXR|Rv2711|MT2784|MTCY05A6.32 iron-dependent repressor from Mycobacterium tuberculosis (230 aa), FASTA scores: opt: 266, E(): 7.1e-10, (27.6% identity in 221 aa overlap). Contains helix-turn-helix motif at aa 32-53 (Score 1327, +3.71 SD). Could belong to the Crp/Fnr family of transcriptional regulators. I6Y1Q2 No regulatory proteins O33330 I6Y1Q2 GO:0005506,GO:0006355,GO:0043565,GO:0003700,GO:0005622 I6Y1Q2 NC_000962.3 Mycobrowser_v5 CDS 3097706 3098938 . - 0 Rv2789c fadE21 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE21 Rv2789c, (MTV002.54c), len: 410 aa. Probable fadE21, acyl-CoA dehydrogenase, similar to many e.g. P45857|ACDB_BACSU|MMGC from Bacillus subtilis (379 aa), FASTA scores: opt: 689, E(): 9.3e-37, (35.75% identity in 400 aa overlap); Q9K6D1|ACDA|BH3798 from Bacillus halodurans (380 aa), FASTA scores: opt: 679, E(): 4.1e-36, (37.3% identity in 405 aa overlap); Q06319|ACDS_MEGEL from Megasphaera elsdenii (383 aa), FASTA scores: opt: 650, E(): 3e-34, (37.7% identity in 334 aa overlap); etc. Contains acyl-CoA dehydrogenases signature 1 (PS00072). Belongs to the acyl-CoA dehydrogenases family. I6XFA9 No lipid metabolism O33331 I6XFA9 GO:0003995,GO:0055114,GO:0050660 1.3.99.- I6XFA9 NC_000962.3 Mycobrowser_v5 CDS 3183138 3183395 . + 0 Rv2871 vapB43 Unknown Possible antitoxin VapB43 Rv2871, (MTCY274.02), len: 85 aa. Possible vapB43, antitoxin, part of toxin-antitoxin (TA) operon with Rv2872, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. O50456|Rv1241|MTV006.13 (86 aa), FASTA scores: opt: 172, E(): 2.9e-05, (37.2% identity in 86 aa overlap); O53811|Rv0748|MTV041.22 (85 aa), FASTA scores: opt: 170, E(): 4e-05, (35.3% identity in 85 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WL41 No virulence, detoxification, adaptation P0A5G9 P9WL41 GO:0006355,GO:0003677 P9WL41 NC_000962.3 Mycobrowser_v5 CDS 4374484 4375683 . - 0 Rv3892c PPE69 Function unknown PPE family protein PPE69 Rv3892c, (MTCY15F10.20), len: 399 aa. PPE69, Member of the Mycobacterium tuberculosis PPE family of conserved proteins, similar to many e.g. O05298|Rv1196|MTCI364.08 from Mycobacterium leprae (391 aa), FASTA scores: opt: 348, E(): 2.2e-08, (26.6% identity in 380 aa overlap). P9WHW7 No PE/PPE Q79F90 P9WHW7 P9WHW7 NC_000962.3 Mycobrowser_v5 CDS 3103257 3103940 . - 0 Rv2794c pptT Biosynthesis of fatty acids and lipids. Transfers the 4'-phosphopantetheine moiety from coenzyme A to a SER of acyl-carrier protein. Catalyzes the formation of holo-ACP, which mediates the transfer of acyl fatty-acid intermediates during the biosynthesis of fatty acids and lipids [catalytic activity: CoA + APO-[acyl-carrier protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] ]. Phosphopantetheinyl transferase PptT (CoA:APO-[ACP]pantetheinephosphotransferase) (CoA:APO-[acyl-carrier protein]pantetheinephosphotransferase) Rv2794c, (MTV002.59c), len: 227 aa. PptT, phosphopantetheinyl transferase, equivalent to Q9Z5I5|ML1547|MLCB596.23 putative iron-chelating complex subunit from Mycobacterium leprae (227 aa), FASTA scores: opt: 1248, E(): 9.1e-77, (79.75% identity in 227 aa overlap). Also highly similar to various proteins e.g. Q9F0Q6|PPTA phosphopantetheinyl transferase from Streptomyces verticillus (246 aa), FASTA scores: opt: 692, E(): 2.8e-39, (46.65% identity in 225 aa overlap); O88029|SC5A7.23 hypothetical 24.5 KDA protein from Streptomyces coelicolor (226 aa), FASTA scores: opt: 679, E(): 2e-38, (46.9% identity in 226 aa overlap); O24813 DNA for L-proline 3-hydroxylase from Streptomyces sp. (208 aa), FASTA scores: opt: 631, E(): 3.2e-35, (48.1% identity in 208 aa overlap); etc. O33336 No lipid metabolism O33336 GO:0009366,GO:0008897,GO:0009059,GO:0000287,GO:0009239 O33336 NC_000962.3 Mycobrowser_v5 CDS 3103937 3104911 . - 0 Rv2795c Rv2795c Function unknown Conserved hypothetical protein Rv2795c, (MTV002.60c), len: 324 aa. Conserved hypothetical protein, equivalent to Q9Z5I6|ML1548|MLCB596.22 hypothetical 37.5 KDA protein from Mycobacterium leprae (321 aa), FASTA scores: opt: 2018, E(): 6.3e-128, (87.4% identity in 318 aa overlap). Also highly similar to O88028|SC5A7.22 hypothetical 33.5 KDA protein from Streptomyces coelicolor (295 aa), FASTA scores: opt: 1202, E(): 3.4e-73, (57.2% identity in 285 aa overlap); and Q9AMH7|SIMX4 SIMX4 protein from Streptomyces antibioticus (293 aa), FASTA scores: opt: 1045, E(): 1.2e-62, (51.4% identity in 286 aa overlap). C-terminus highly similar to Q9F0Q7 hypothetical 9.6 KDA protein (fragment) from Streptomyces verticillus (81 aa), FASTA scores: opt: 395, E(): 1.8e-19, (68.35% identity in 79 aa overlap). Also similar to other proteins e.g. Q9FWV7 hypothetical 45.3 KDA protein from Oryza sativa (Rice) (402 aa), FASTA scores: opt: 294, E(): 3.6e-12, (26.45% identity in 340 aa overlap). I6YEE1 No conserved hypotheticals O33337 I6YEE1 GO:0016787 I6YEE1 NC_000962.3 Mycobrowser_v5 CDS 3105619 3107307 . - 0 Rv2797c Rv2797c Function unknown Conserved hypothetical protein Rv2797c, (MTCY16B7.46), len: 562 aa. Conserved hypothetical ala-rich protein. C-terminus highly similar to several mycobacterial proteins e.g. AAK46927|MT2616 hypothetical 28.0 KDA protein from Mycobacterium tuberculosis strain CDC1551 (265 aa), FASTA scores: opt: 535, E(): 4.6e-22, (42.95% identity in 263 aa overlap); P95011|Rv2542|MTCY159.14c hypothetical 42.4 KDA protein from Mycobacterium tuberculosis (403 aa), FASTA scores: opt: 537, E(): 5e-22, (40.75% identity in 292 aa overlap) (similarity in the second half of protein); P71547|Y963_MYCTU|Rv0963c|MT0992|MTCY10D7.11 hypothetical 28.1 KDA protein (266 aa), FASTA scores: opt: 314, E(): 5.7e-10, (39.0% identity in 254 aa overlap); etc. Contains PS00120 Lipases, serine active site. P71654 No conserved hypotheticals P71654 P71654 P71654 NC_000962.3 Mycobrowser_v5 CDS 3183905 3184567 . + 0 Rv2873 mpt83 Not really known. Cell surface lipoprotein Mpt83 (lipoprotein P23) Rv2873, (MTCY274.04), len: 220 aa. Mpt83 (alternate gene name: mpb83), cell surface lipoprotein (see citations below). Also similar to upstream ORF Q50769|MP70_MYCTU|MPT70|MPB70|Rv2875|MT2943|MTCY274.06 which is also known as major secreted immunogenic protein MPT70 precursor from Mycobacterium tuberculosis (193 aa), FASTA scores: opt: 806, E(): 2.7e-38, (70.25% identity in 185 aa overlap). Belongs to the MPT70 / MPT83 family. Attached to the membrane by a lipid anchor. P9WNF3 No cell wall and cell processes P0A670 P9WNF3 GO:0005886 P9WNF3 NC_000962.3 Mycobrowser_v5 CDS 3110167 3110523 . - 0 Rv2801c mazF9 Sequence-specific mRNA cleavage Toxin MazF9 Rv2801c, (MTCY16B7.42), len: 118 aa. MazF9, toxin, part of toxin-antitoxin (TA) operon with Rv2801A (See Pandey and Gerdes, 2005; Zhu et al., 2006), highly similar to Q9RWK4|DR0662 conserved hypothetical protein from Deinococcus radiodurans (115 aa), FASTA scores: opt: 306, E(): 2e-15, (43.95% identity in 116 aa overlap); and similar to AAK78474|CAC0494 PEMK family of DNA-binding proteins from Clostridium acetobutylicum (122 aa), FASTA scores: opt: 217, E(): 7.3e-09, (33.35% identity in 117 aa overlap); P96622|YDCE YDCE protein from Bacillus subtilis (116 aa), FASTA scores: opt: 194, E(): 3.5e-07, (33.35% identity in 117 aa overlap); Q9PHH8|XFA0027 plasmid maintenance protein from Xylella fastidiosa (108 aa), FASTA scores: opt: 188, E(): 9.1e-07, (40.85% identity in 115 aa overlap); etc. Also similar to Q10867|YJ91_MYCTU|Rv1991c|MT2046|MTCY39.28 hypothetical 12.3 KDA protein from Mycobacterium tuberculosis (114 aa), FASTA scores: opt: 190, E(): 6.8e-07, (36.75% identity in 117 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P71650 No virulence, detoxification, adaptation P71650 P71650 GO:0003677 P71650 NC_000962.3 Mycobrowser_v5 CDS 3110780 3111823 . - 0 Rv2802c Rv2802c Unknown Hypothetical arginine and alanine rich protein Rv2802c, (MTCY16B7.41), len: 347 aa. Hypothetical unknown arg-, ala-rich protein. C-terminus shows some similarity with N-terminal part of hypothetical proteins Q98K84|MLR1592 from Rhizobium loti (Mesorhizobium loti) (104 aa), FASTA scores: opt: 138, E(): 0.12, (37.35% identity in 91 aa overlap); and CAC47718|SMC03294 from Rhizobium meliloti (Sinorhizobium meliloti) (114 aa), FASTA scores: opt: 128, E(): 0.53, (31.4% identity in 86 aa overlap). Equivalent to AAK47191 from Mycobacterium tuberculosis strain CDC1551 (357 aa) but shorter 10 aa. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P71649 No conserved hypotheticals P71649 P71649 NC_000962.3 Mycobrowser_v5 CDS 3111822 3112289 . + 0 Rv2803 Rv2803 Function unknown Conserved hypothetical protein Rv2803, len: 155 aa. Conserved hypothetical protein, similar to hypothetical proteins from other organisms, and with some similarity to C-terminal part of Rv0918|Z95210_12 hypothetical protein from Mycobacterium tuberculosis (158 aa), FASTA scores: opt: 204, E(): 9e-07, (42.35% identity in 85 aa overlap). Replaces original 2803c on other strand. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6XFC2 No conserved hypotheticals Q8VJD2 I6XFC2 I6XFC2 NC_000962.3 Mycobrowser_v5 CDS 3112465 3113094 . - 0 Rv2804c Rv2804c Unknown Hypothetical protein Rv2804c, (MTCY16B7.39), len: 209 aa. Hypothetical unknown protein, overlaps neighbouring orf Rv2805|MTCY16B7.38c. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6YEE9 No conserved hypotheticals I6YEE9 NC_000962.3 Mycobrowser_v5 CDS 3112867 3113271 . + 0 Rv2805 Rv2805 Function unknown Conserved hypothetical protein Rv2805, (MTCY16B7.38c), len: 134 aa. Conserved hypothetical protein, highly similar to N-terminal region of downstream ORF P71644|Rv2807|MTCY16B7.36c conserved hypothetical protein from Mycobacterium tuberculosis (384 aa), FASTA scores: opt: 525, E(): 6.4e-29, (78.2% identity in 101 aa overlap). Also highly similar to N-terminus of other proteins: Q9KK74 hypothetical 47.4 KDA protein from Brevibacterium linens (418 aa), FASTA scores: opt: 480, E(): 8.8e-26, (64.15% identity in 106 aa overlap); AAK40065 Rv3128c-like protein from Mycobacterium celatum (423 aa), FASTA scores: opt: 218, E(): 1.2e-07, (46.05% identity in 89 aa overlap); Q981U5|MLR9230 from Rhizobium loti (Mesorhizobium loti) (504 aa), FASTA scores: opt: 131, E(): 0.15, (29.4% identity in 126 aa overlap). Overlaps neighbouring ORF Rv2804c|MTCY16B7.39. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P71646 No conserved hypotheticals P71646 NC_000962.3 Mycobrowser_v5 CDS 3113268 3113459 . + 0 Rv2806 Rv2806 Unknown Possible membrane protein Rv2806, (MTCY16B7.37c), len: 63 aa. Possible membrane protein, sharing no homology. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6YAA5 No cell wall and cell processes I6YAA5 NC_000962.3 Mycobrowser_v5 CDS 3379036 3379329 . - 0 Rv3020c esxS Function unknown ESAT-6 like protein EsxS Rv3020c, (MTV012.34c), len: 97 aa. EsxS, ESAT-6 like protein. PE-family related protein; distant member of the Mycobacterium tuberculosis PE family, similar to AAK44524|MT0300 PE family protein from M. tuberculosis strain CDC1551 (97 aa), FASTA scores: opt: 564, E(): 5.9e-30, (91.75% identity in 97 aa overlap). Has potential helix-turn-helix motif at positions 14-35. Seems to belong to the ESAT6 family (see Betts et al., 2002). Note that previously known as PE28. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Q6MX18 No cell wall and cell processes 3H6P Q6MX18 Q6MX18 NC_000962.3 Mycobrowser_v5 CDS 3115046 3115303 . + 0 Rv2808 Rv2808 Unknown Hypothetical protein Rv2808, (MTCY16B7.35c), len: 85 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P71643 No conserved hypotheticals P71643 NC_000962.3 Mycobrowser_v5 CDS 3115741 3116142 . - 0 Rv2810c Rv2810c Required for the transposition of the insertion element IS1555. Probable transposase Rv2810c, (MTCY16B7.33), len: 133 aa. Probable transposase for IS1555, similar to C-terminal domain of transposases for defective IS1555 e.g. Q9LCS0|TNPA transposase from Arthrobacter sp. TM1 (435 aa), FASTA scores: opt: 294, E(): 1.8e-13, (55.1% identity in 98 aa overlap); Q50440|TNPA insertion element TNPR and TNPA gene from Mycobacterium smegmatis (413 aa), FASTA scores: opt: 274, E(): 4.7e-12, (56.25% identity in 96 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P71641 No insertion seqs and phages P71641 P71641 NC_000962.3 Mycobrowser_v5 CDS 3116139 3116747 . + 0 Rv2811 Rv2811 Function unknown Conserved hypothetical protein Rv2811, (MTCY16B7.32c), len: 202 aa. Conserved hypothetical protein. C-terminus equivalent to C-terminus of AAK47198|MT2878 hypothetical 17.7 KDA protein Mycobacterium tuberculosis strain CDC1551 (178 aa), FASTA scores: opt: 609, E(): 1.5e-32, (61.0% identity in 182 aa overlap); and C-terminus highly similar to P72038|Rv3771c|MTCY13D12.05c hypothetical 11.3 KDA protein from Mycobacterium tuberculosis (108 aa), FASTA scores: opt: 465, E(): 2.8e-23, (73.6% identity in 106 aa overlap). Also some similarity with P71962|Rv2665|MTCY441.34 hypothetical 10.5 KDA protein from Mycobacterium tuberculosis (93 aa), FASTA scores: opt: 153, E(): 0.0057, (39.05% identity in 64 aa overlap); and Q9A6W6|CC1966 hypothetical protein CC1966 from Caulobacter crescentus (189 aa), FASTA scores: opt: 115, E(): 2.6, (39.4% identity in 104 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P71640 No conserved hypotheticals P71640 P71640 NC_000962.3 Mycobrowser_v5 CDS 3116818 3118227 . + 0 Rv2812 Rv2812 Required for the transposition of the insertion element IS1604. Probable transposase Rv2812, (MTCY16B7.31c), len: 469 aa. Probable transposase for IS1604, similar to putative transposases and hypothetical proteins e.g. Q9EZM2|putative transposase from Mycobacterium paratuberculosis (395 aa), FASTA scores: opt: 329, E(): 3e-13, (27.05% identity in 362 aa overlap); CAC46499 putative transposase protein from Rhizobium meliloti (Sinorhizobium meliloti) (390 aa), FASTA scores: opt: 327, E(): 3.9e-13, (30.5% identity in 367 aa overlap); etc. Contains possible helix-turn-helix motif at aa 50-71 (Score 1140, +3.07 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P71639 No insertion seqs and phages P71639 P71639 GO:0015074,GO:0003677 P71639 NC_000962.3 Mycobrowser_v5 CDS 3129344 3131773 . - 0 Rv2823c Rv2823c Function unknown Conserved protein Rv2823c, (MTCY16B7.19), len: 809 aa. Conserved protein, similar in part to others e.g. Q9X2D1|TM1811Thermotoga maritima (717 aa), FASTA scores: opt: 401, E(): 3.6e-18, (27.15% identity in 773 aa overlap); O27154|MTH1082 conserved hypothetical protein from Methanothermobacter thermautotrophicus (822 aa), FASTA scores: opt: 306, E(): 6e-12, (25.55% identity in 872 aa overlap); Q59066|MJ1672 hypothetical protein from Methanococcus jannaschii (800 aa), FASTA scores: opt: 302, E(): 1.1e-11, (24.9% identity in 812 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P71629 No conserved hypotheticals P71629 NC_000962.3 Mycobrowser_v5 CDS 3136620 3137012 . - 0 Rv2829c vapC22 Unknown Possible toxin VapC22 Rv2829c, (MTCY16B7.13), len: 130 aa. Possible vapC22, toxin, part of toxin-antitoxin (TA) operon with Rv2830c, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Conserved hypothetical protein similar to AAK65872|SMA2253 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (125 aa), FASTA scores: opt: 171, E(): 7.7e-05, (34.9% identity in 129 aa overlap); and shows some similarity with other proteins e.g. Q9AH69 hypothetical 14.7 KDA protein from Neisseria meningitidis (128 aa), FASTA scores: opt: 148, E(): 0.0031, (28.1% identity in 121 aa overlap). P71623 No virulence, detoxification, adaptation P71623 P71623 P71623 NC_000962.3 Mycobrowser_v5 CDS 3123967 3124983 . - 0 Rv2817c Rv2817c Function unknown Conserved hypothetical protein Rv2817c, (MTCY16B7.26), len: 338 aa. Conserved hypothetical protein, showing similarity with O30236|AF2435 conserved hypothetical protein from Archaeoglobus fulgidus (322 aa), FASTA scores: opt: 397, E(): 2.4e-19, (28.2% identity in 298 aa overlap); Q9KFX9|BH0341 hypothetical protein from Bacillus halodurans (343 aa), FASTA scores: opt: 337, E(): 2.8e-15, (27.35% identity in 300 aa overlap); Q9X2B7|TM1797 conserved hypothetical protein from Thermotoga maritima (319 aa), FASTA scores: opt: 321, E(): 3.3e-14, (26.5% identity in 268 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WPJ5 No conserved hypotheticals P71636 P9WPJ5 P9WPJ5 NC_000962.3 Mycobrowser_v5 CDS 3124996 3126144 . - 0 Rv2818c Rv2818c Unknown Hypothetical protein Rv2818c, (MTCY16B7.25), len: 382 aa. Hypothetical unknown protein, equivalent to AAK47210 from Mycobacterium tuberculosis strain CDC1551 (430 aa) but shorter 48 aa. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P71635 No unknown P71635 P71635 NC_000962.3 Mycobrowser_v5 CDS 3132892 3133539 . - 0 Rv2825c Rv2825c Function unknown Conserved hypothetical protein Rv2825c, (MTCY16B7.17), len: 215 aa. Conserved hypothetical protein, similar to Q9RY53|DR0097 conserved hypothetical protein from Deinococcus radiodurans (189 aa), FASTA scores: opt: 261, E(): 8e-11, (33.5% identity in 176 aa overlap); and shows some similarity with N-terminus of O27278|MTH1210 MRR restriction system related protein from Methanothermobacter thermautotrophicus (340 aa), FASTA scores: opt: 133, E(): 0.091, (28.55% identity in 112 aa overlap). Equivalent to AAK47217 from Mycobacterium tuberculosis strain CDC1551 (246 aa) but shorter 31 aa; and equivalent to upstream ORF P71624|Rv2828c|MTCY16B7.14 from Mycobacterium tuberculosis strain H37Rv (alias AAK47221 from strain CDC1551) (181 aa), FASTA scores: opt: 1169, E(): 8.5e-74, (98.35% identity in 181 aa overlap). P71627 No conserved hypotheticals P71627 P71627 NC_000962.3 Mycobrowser_v5 CDS 3133709 3134593 . - 0 Rv2826c Rv2826c Unknown Hypothetical protein Rv2826c, (MTCY16B7.16), len: 294 aa. Hypothetical unknown protein. P71626 No conserved hypotheticals P71626 P71626 NC_000962.3 Mycobrowser_v5 CDS 3501794 3502936 . + 0 Rv3136 PPE51 Function unknown PPE family protein PPE51 Rv3136, (MTCY03A2.22c), len: 380 aa. PPE51, Member of the Mycobacterium tuberculosis Ala-, Gly-rich PPE family, similar to Q9AGF0|Ov2770c Rv2770c-like protein from M. microti (397 aa), FASTA scores: opt: 917, E(): 9e-41, (46.15% identity in 388 aa overlap); O33312|Rv2770c|MTV002.35c, MTV002_36, MTCI125_26, MTCY10G2_10, MTCI364_8, MTV049_28, MTV049_29, etc. P9WHY3 No PE/PPE Q7D623 P9WHY3 P9WHY3 NC_000962.3 Mycobrowser_v5 CDS 3139174 3140484 . - 0 Rv2833c ugpB Involved in active transport of Sn-glycerol-3-phosphate and glycerophosphoryl diesters across the membrane (import). Sn-glycerol-3-phosphate and glycerophosphoryl diesters - binding protein interacts with the binding protein-dependent transport system ugpace. Probable Sn-glycerol-3-phosphate-binding lipoprotein UgpB Rv2833c, (MTCY16B7.09), len: 436 aa. Probable ugpB, Sn-glycerol-3-phosphate binding lipoprotein component of Sn-glycerol-3-phosphate transport system (see citation below), similar to various transporters substrate-binding periplasmic proteins e.g. Q9KDY2|BH1079 glycerol-3-phosphate ABC transporter (glycerol-3-phosphate binding protein) from Bacillus halodurans (459 aa), FASTA scores: opt: 357, E(): 3.1e-14, (23.4% identity in 406 aa overlap); P72397|male putative maltose-binding protein from Streptomyces coelicolor (423 aa), FASTA scores: opt: 318, E(): 7e-12, (23.7% identity in 430 aa overlap); AAK78409|CAC0429 glycerol-3-phosphate ABC-transporter periplasmic component from Clostridium acetobutylicum (447 aa), FASTA scores: opt: 305, E(): 4.5e-11, (27.15% identity in 438 aa overlap); P10904|UGPB_ECOLI|B3453 glycerol-3-phosphate-binding periplasmic protein precursor from Escherichia coli strain K12 (438 aa); etc. Contains signal sequence and appropriately positioned prokaryotic lipoprotein attachment site (PS00013). P71619 No cell wall and cell processes P71619 P71619 GO:0005215,GO:0006810 P71619 NC_000962.3 Mycobrowser_v5 CDS 3174059 3174643 . - 0 Rv2862c Rv2862c Function unknown Conserved hypothetical protein Rv2862c, (MTV003.08), len: 194 aa. Conserved hypothetical protein, showing some similarity with others e.g. Q9X8X5|SCH35.31c hypothetical 19.6 KDA protein from Streptomyces coelicolor (180 aa), FASTA scores: opt: 266, E(): 2.2e-11, (34.65% identity in 179 aa overlap); Q9Z5H1|ML0169|MLCB373.19 hypothetical 22.1 KDA protein from Mycobacterium leprae (200 aa), FASTA scores: opt: 195, E(): 2.3e-06, (30.15% identity in 189 aa overlap); etc. Also some similarity to P71544|Y966_MYCTU|Rv0966c|MT0994|MTCY10D7.08 conserved hypothetical protein from Mycobacterium tuberculosis (230 aa), FASTA scores: opt: 209, E(): 2.6e-07, (31.5% identity in 184 aa overlap). I6Y1W7 No conserved hypotheticals O33344 I6Y1W7 NC_000962.3 Mycobrowser_v5 CDS 3142309 3143628 . - 0 Rv2836c dinF Function unknown; induction by DNA damage. Possible DNA-damage-inducible protein F DinF Rv2836c, (MTCY16B7.06), len: 439 aa. Possible dinF, DNA-damage-inducible protein F, integral membrane protein, similar to others e.g. BAB38450|ECS5027|AAG59243 from Escherichia coli strain O157:H7 (459 aa), FASTA scores: opt: 501, E(): 2.7e-21, (29.55% identity in 443 aa overlap); P28303|DINF_ECOLI|B4044 from Escherichia coli strain K12 (459 aa), FASTA scores: opt: 491, E(): 1e-20, (29.35% identity in 443 aa overlap); Q98B90|MLR5680 from Rhizobium loti (Mesorhizobium loti) (471 aa), FASTA scores: opt: 466, E(): 2.7e-19, (30.7% identity in 433 aa overlap); etc. But also similar or highly similar to other hypothetical proteins e.g. Q9X8U6|SCH24.32c hypothetical 46.3 KDA protein from Streptomyces coelicolor (448 aa), FASTA scores: opt: 981, E(): 1.1e-48, (42.35% identity in 437 aa overlap). Contains PS00213 Lipocalin signature. P71616 No information pathways P71616 P71616 GO:0006855,GO:0015238,GO:0016021,GO:0015297 NC_000962.3 Mycobrowser_v5 CDS 3856911 3857387 . + 0 Rv3437 Rv3437 Unknown Possible conserved transmembrane protein Rv3437, (MTCY77.09), len: 158 aa. Questionable ORF. Possible conserved transmenbrane protein, C-terminus similar to N-terminal part of O06345|Rv3482c|MTCY13E12.35c hypothetical 28.5 KDA protein from Mycobacterium tuberculosis (260 aa), FASTA scores: opt: 140, E(): 0.1, (58.8% identity in 34 aa overlap); and Q9XAN5|SC4C6.05c putative membrane protein from Streptomyces (347 aa), coelicolor FASTA scores: opt: 112, E(): 6.8, (50.0% identity in 32 aa overlap). I6YG51 No cell wall and cell processes O06254 I6YG51 GO:0016021 I6YG51 NC_000962.3 Mycobrowser_v5 CDS 3143635 3144645 . - 0 Rv2837c Rv2837c Function unknown Conserved protein Rv2837c, (MTCY16B7.05), len: 336 aa. Conserved protein, showing some similarity with other proteins e.g. O67552|AQ_1630 hypothetical 36.2 KDA protein from Aquifex aeolicus (325 aa), FASTA scores: opt: 498, E(): 3.6e-25, (32.8% identity in 314 aa overlap); Q9X1T1|TM1595 conserved hypothetical protein from Thermotoga maritima (333 aa), FASTA scores: opt: 482, E(): 4.1e-24, (34.85% identity in 304 aa overlap); Q9RW43|DR0826 conserved hypothetical protein from Deinococcus radiodurans (338 aa), FASTA scores: opt: 444, E(): 1.3e-21, (33.85% identity in 331 aa overlap); etc. Equivalent to AAK47229 from Mycobacterium tuberculosis strain CDC1551 (316 aa) but longer 20 aa. P71615 No conserved hypotheticals P71615 P71615 GO:0030145,GO:0003676,GO:0016787 P71615 NC_000962.3 Mycobrowser_v5 CDS 3144620 3145171 . - 0 Rv2838c rbfA Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Essential for efficient processing of 16S rRNA. May interact with the 5'terminal helix region of 16S SRNA. Probable ribosome-binding factor a RbfA (P15B protein) Rv2838c, (MTCY16B7.04), len: 183 aa. Probable rbfA, ribosome-binding factor A, equivalent to Q9Z5I8|RBFA_MYCLE|ML1555|MLCB596.15 probable ribosome-binding factor a from Mycobacterium leprae (164 aa), FASTA scores: opt: 739, E(): 1.8e-40, (75.6% identity in 160 aa overlap). Also highly similar or similar to others e.g. Q9Z527|RBFA_STRCO|SC9F2.08c from Streptomyces coelicolor (160 aa), FASTA scores: opt: 425, E(): 2.8e-20, (50.35% identity in 141 aa overlap); P32731|RBFA_BACSU from Bacillus subtilis (117 aa), FASTA scores: opt: 199, E(): 7.8e-06, (32.4% identity in 108 aa overlap); P09170|RBFA_ECOLI|P15B|B3167 from Escherichia coli strain K12 (132 aa), FASTA scores: opt: 166, E(): 0.0011, (29.65% identity in 118 aa overlap); etc. Belongs to the RBFA family. Note that appears to be longer in C-terminus than other RbfA proteins. P9WHJ7 No information pathways P65964 P9WHJ7 GO:0006364,GO:0005737 P9WHJ7 NC_000962.3 Mycobrowser_v5 CDS 3145171 3147873 . - 0 Rv2839c infB If-2, one of the essential components for the initiation of protein synthesis in vitro, protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. It is also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex. Probable translation initiation factor if-2 InfB Rv2839c, (MTCY16B7.03), len: 900 aa. Probable infB, translation initiation factor if-2, highly similar, but in part, to Q9Z5I9|IF2_MYCLE|ML1556|MLCB596.14 translation initiation factor if-2 from Mycobacterium leprae (924 aa), FASTA scores: opt: 4548, E(): 2.4e-132, (83.6% identity in 933 aa overlap). Also similar in part to others e.g. Q9K3E2|SC5H4.30 from Streptomyces coelicolor (835 aa), FASTA scores: opt: 2559, E(): 1.3e-71, (59.9% identity in 833 aa overlap); P17889|IF2_BACSU|INFB from Bacillus subtilis (716 aa), FASTA scores: opt: 1782, E(): 6.6e-48, (46.65% identity in 686 aa overlap); P02995|IF2_ECOLI|INFB|SSYG|B3168|Z4529|ECS4049 from Escherichia coli strains O157:H7 and K12 (890 aa), FASTA scores: opt: 1708, E(): 1.3e-45, (46.2% identity in 662 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the if-2 family. P9WKK1 No information pathways P65131 P9WKK1 GO:0003924,GO:0005737,GO:0003743,GO:0006413,GO:0005525 P9WKK1 NC_000962.3 Mycobrowser_v5 CDS 4096139 4096939 . - 0 Rv3658c Rv3658c Unknown Probable conserved transmembrane protein Rv3658c, (MTV025.006c), len: 266 aa. Probable conserved transmembrane protein, similar to Q9X920|SCH5.18c putative integral membrane protein from Streptomyces coelicolor (321 aa), FASTA scores: opt: 335, E(): 4.1e-13, (38.05% identity in 247 aa overlap). I6Y479 No cell wall and cell processes O69626 I6Y479 GO:0016021 I6Y479 NC_000962.3 Mycobrowser_v5 CDS 3149425 3149976 . - 0 Rv2842c Rv2842c Function unknown Conserved protein Rv2842c, (MTCY24A1.15), len: 183 aa. Conserved protein, similar to Q9Z5J2|MLCB596.11 hypothetical 13.7 KDA protein from Mycobacterium leprae (122 aa), FASTA scores: opt: 192, E(): 2.1e-12, (50.0% identity in 128 aa overlap) (N-terminus shorter). Also similar in part to several hypothetical proteins e.g. Q9KYR2|SC5H4.27 hypothetical 19.8 KDA protein from Streptomyces coelicolor (177 aa), FASTA scores: opt: 288, E(): 2.1e-12, (37.15% identity in 148 aa overlap); O66619|Y260_AQUAE|AQ_260 hypothetical protein from Aquifex aeolicus (158 aa), FASTA scores: opt: 230, E(): 1.7e-08, (31.35% identity in 153 aa overlap); Q9KU82|VC0641 hypothetical protein from Vibrio cholerae (151 aa), FASTA scores: opt: 198, E(): 2.5e-06, (30.9% identity in 152 aa overlap); etc. P9WH17 No conserved hypotheticals P67214 P9WH17 GO:0042274,GO:0005737 P9WH17 NC_000962.3 Mycobrowser_v5 CDS 3150171 3150716 . + 0 Rv2843 Rv2843 Unknown Probable conserved transmembrane alanine rich protein Rv2843, (MTCY24A1.14c), len: 181 aa. Probable conserved transmembrane ala-rich protein, equivalent to Q9Z5J3|ML1560|MLCB596.10c hypothetical 17.5 KDA protein from Mycobacterium leprae (178 aa), FASTA scores: opt: 707, E(): 1.4e-32, (70.25% identity in 168 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). I6YAE2 No cell wall and cell processes I6YAE2 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 3150713 3151201 . + 0 Rv2844 Rv2844 Function unknown Conserved alanine rich protein Rv2844, (MTCY24A1.13c), len: 162 aa. Conserved ala-rich protein, equivalent to Q9Z5J4|ML1561|MLCB596.09c hypothetical 17.5 KDA protein from Mycobacterium leprae (165 aa), FASTA scores: opt: 771, E(): 4.9e-46, (71.5% identity in 165 aa overlap). Also similar to Q9KYR4|SC5H4.25c hypothetical 16.8 KDA protein from Streptomyces coelicolor (167 aa), FASTA scores: opt: 242, E(): 1.6e-09, (38.9% identity in 144 aa overlap). I6Y1V1 No conserved hypotheticals 2IB0 I6Y1V1 I6Y1V1 NC_000962.3 Mycobrowser_v5 CDS 3162268 3164115 . + 0 Rv2853 PE_PGRS48 Function unknown PE-PGRS family protein PE_PGRS48 Rv2853, (MTCY24A1.04c), len: 615 aa. PE_PGRS48, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to many e.g. O53884|Rv0872c|MTV043.65c from Mycobacterium tuberculosis (606 aa), FASTA scores: opt: 1405, E(): 1.4e-97, (64.6% identity in 619 aa overlap). Equivalent to AAK47245 from Mycobacterium tuberculosis strain CDC1551 (663 aa) but shorter 48 aa. Q6MX26 No PE/PPE Q6MX26 Q6MX26 Q6MX26 NC_000962.3 Mycobrowser_v5 CDS 3267737 3272203 . + 0 Rv2935 ppsE Involved in phenolpthiocerol and phthiocerol dimycocerosate (dim) biosynthesis: extension with malony CoA (partial reduction, decarboxylation). Phenolpthiocerol synthesis type-I polyketide synthase PpsE Rv2935, (MTCY19H9.03), len: 1488 aa. PpsE, type-I polyketide synthase (see citations below). Contains PS00606 Beta-ketoacyl synthases active site. Note that Rv2935|ppsE belongs to the transcriptional unit Rv2930|fadD26-Rv2939|papA5 (proven experimentally). TesA|Rv2928 interacts with PpsE|Rv2935, by bacterial two-hybrid and GST-pulldown assays (See Rao and Ranganathan, 2004). P9WQE1 No lipid metabolism P96204 P9WQE1 GO:0009058,GO:0048037,GO:0016740,GO:0000036 P9WQE1 NC_000962.3 Mycobrowser_v5 CDS 3153039 3154631 . - 0 Rv2846c efpA Thought to be involved in transport of undetermined substrate (possibly drug) across the membrane (export): so responsible for the translocation of the substrate across the membrane. Possible integral membrane efflux protein EfpA Rv2846c, (MTCY24A1.11), len: 530 aa. Possible efpA, integral membrane efflux protein, member of major facilitator superfamily (MFS) possibly involved in transport of drug (see citations below), equivalent to Q9Z5J5|ML1562|MLCB596.08 putative transmembrane efflux protein from Mycobacterium leprae (534 aa), FASTA scores: opt: 2881, E(): 4.1e-160, (86.55% identity in 535 aa overlap). Also highly similar to several membrane proteins e.g. O69986|SC4H2.31c transmembrane efflux protein (515 aa), FASTA scores: opt: 1063, E(): 2.2e-54, (39.65% identity in 406 aa overlap); Q9FBQ5|SCD86A.02c putative transport integral membrane protein from Streptomyces coelicolor (503 aa), FASTA scores: opt: 918, E(): 5.8e-46, (33.7% identity in 469 aa overlap); Q9KYU0|SCE22.23c putative transmembrane efflux protein from Streptomyces coelicolor (514 aa), FASTA scores: opt: 888, E(): 3.3e-44, (32.85% identity in 469 aa overlap); etc. P9WJY5 No cell wall and cell processes O05813 P9WJY5 GO:0005886,GO:0055085,GO:0016021 INH NC_000962.3 Mycobrowser_v5 CDS 3154654 3155871 . - 0 Rv2847c cysG Involved in the biosynthesis of siroheme and cobalamin [catalytic activity: 2 S-adenosyl-L-methionine + uroporphyrin III = 2 S-adenosyl-L-homocysteine + sirohydrochlorin]. SAM-dependent methyl transferase that methylates uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It catalyzes also the conversion of precorrin-2 into siroheme (consisting of an oxidation and FE(2+) chelation). Possible multifunctional enzyme siroheme synthase CysG: uroporphyrin-III C-methyltransferase (urogen III methylase) (SUMT) (uroporphyrinogen III methylase) (UROM) + precorrin-2 oxidase + ferrochelatase Rv2847c, (MTCY24A1.10), len: 405 aa. Possible cysG, multifunctional enzyme, siroheme synthase containing uroporphyrin-III c-methyltransferase, precorrin-2 oxidase and ferrochelatase. C-terminus highly similar to many uroporphyrin-III c-methyltransferases e.g. Q51720|COBA uroporphyrinogen III methyltransferase from Propionibacterium freudenreichii (257 aa), FASTA scores: opt: 776, E(): 1.5e-39, (48.95% identity in 243 aa overlap); Q9HMY4|UROM|VNG2331G S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase from Halobacterium sp. strain NRC-1 (246 aa), FASTA scores: opt: 704, E(): 3.1e-35, (49.4% identity in 245 aa overlap); P42437|NASF_BACSU|NASBE uroporphyrin-III C-methyltransferase from Bacillus subtilis (483 aa), FASTA scores: opt: 610, E(): 2.4e-29, (42.1% identity in 240 aa overlap); etc. And highly similar over entire length to other proteins e.g. Q9L1C9|SCL11.09c uroporphyrinogen III methyltransferase from Streptomyces coelicolor (410 aa), FASTA scores: opt: 1481, E(): 5.6e-82, (58.45% identity in 409 aa overlap); Q9I0M7|CYSG|PA2611 siroheme synthase from Pseudomonas aeruginosa (465 aa), FASTA scores: opt: 609, E(): 2.7e-29, (34.7% identity in 444 aa overlap); P11098|CYSG_ECOLI|B3368|Z4729|ECS4219 siroheme synthase from Escherichia coli stains O157:H7 and K12 (457 aa), FASTA scores: opt: 543, E(): 9.1e-27, (31.3% identity in 450 aa overlap); etc. Belongs to a family that groups SUMT, CYSG, CBIF/COBM and CBIL/COBI. Note that previously known as cysG2. I6X5I7 No intermediary metabolism and respiration P95077 I6X5I7 GO:0016829,GO:0055114,GO:0016491,GO:0006779,GO:0004851 2.1.1.107,4.99.1.-,1.-.-.- I6X5I7 NC_000962.3 Mycobrowser_v5 CDS 3158165 3160054 . - 0 Rv2850c Rv2850c Function unknown; possibly introduces a magnesium ion into specific substrate/compound. Possible magnesium chelatase Rv2850c, (MTCY24A1.07), len: 629 aa. Possible magnesium-chelatase, highly similar (but with gaps) to magnesium-chelatases from notably photosynthetic organisms involved in chlorophyll biosynthesis e.g. Q9RJ18|SCI8.35c putative chelatase from Streptomyces coelicolor (672 aa), FASTA scores: opt: 1941, E(): 2.1e-85, (54.65% identity in 675 aa overlap); Q9HZQ5|PA2942 probable magnesium chelatase from Pseudomonas aeruginosa (338 aa), FASTA scores: opt: 991, E(): 2.7e-40, (49.45% identity in 368 aa overlap); O33549|BCHI mg protoporphyrin IX chelatase subunit from Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides) (334 aa), FASTA scores: opt: 833, E(): 9.4e-33, (50.65% identity in 318 aa overlap); O30819|BCHI_RHOSH magnesium-chelatase 38 KDA subunit from Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides) (334 aa), FASTA scores: opt: 828, E(): 1.6e-32, (50.3% identity in 318 aa overlap); etc. Equivalent to AAK47242 from Mycobacterium tuberculosis strain CDC1551 (610 aa) but longer 19 aa. COULB belong to the mg-chelatase subunits D/I family. P9WPR3 No intermediary metabolism and respiration O05809 P9WPR3 GO:0016887,GO:0005524 P9WPR3 NC_000962.3 Mycobrowser_v5 CDS 3468413 3469264 . - 0 Rv3099c Rv3099c Function unknown Conserved protein Rv3099c, (MTCY164.10c), len: 283 aa. Conserved protein, some similarity with hypothetical proteins e.g. Q9XA69|SCGD3.09 from Streptomyces coelicolor (274 aa), FASTA scores: opt: 384, E(): 1.8e-17, (32.7% identity in 269 aa overlap); and P71606|Y036_MYCTU|Rv0036c from Mycobacterium tuberculosis strain H37Rv (257 aa), FASTA scores: opt: 179, E(): 0.00024, (25.85% identity in 205 aa overlap). O05777 No conserved hypotheticals O05777 O05777 O05777 NC_000962.3 Mycobrowser_v5 CDS 3157521 3158144 . - 0 Rv2849c cobO Involved in cobalamin biosynthesis; transforms cobyrinic acid into cobinamide [catalytic activity: ATP + cob(I)alamin + H(2)O = orthophosphate + pyrophosphate + adenosylcobalamin]. Probable cob(I)alamin adenosyltransferase CobO (corrinoid adenosyltransferase) (corrinoid adotransferase activity) Rv2849c, (MTCY24A1.08), len: 207 aa. Probable cobO, cob(I)alamin adenosyltransferase, highly similar to Q9RJ17|COBO from Streptomyces coelicolor (199 aa), FASTA scores: opt: 918, E(): 1.1e-55, (64.75% identity in 207 aa overlap); and similar to others e.g. O30785|COBO from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (212 aa), FASTA scores: opt: 329, E(): 2.8e-15, (44.3% identity in 185 aa overlap); P29930|COBO_PSEDE from Pseudomonas denitrificans (213 aa), FASTA scores: opt: 280, E(): 6.5e-12, (38.9% identity in 185 aa overlap); P31570|BTUR_SALTY|COBA from Salmonella typhimurium (196 aa), FASTA scores: opt: 278, E(): 8.4e-12, (39.8% identity in 196 aa overlap); etc. Cofactor: manganese. Note that previously known as cobA. I6Y1V6 No intermediary metabolism and respiration Q7D6G6 I6Y1V6 GO:0008817,GO:0009236,GO:0005524 2.5.1.17 I6Y1V6 NC_000962.3 Mycobrowser_v5 CDS 3160051 3160521 . - 0 Rv2851c Rv2851c Acetylation, substrate unknown GCN5-related N-acetyltransferase Rv2851c, (MTCY24A1.06), len: 156 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Similar to others e.g. Q9KP14|VC2565 ELAA protein from Vibrio cholerae (149 aa), FASTA scores: opt: 360, E(): 1e-18, (46.05% identity in 139 aa overlap); Q9I717|PA0115 hypothetical protein from Pseudomonas aeruginosa (150 aa), FASTA scores: opt: 341, E(): 2.4e-17, (43.65% identity in 142 aa overlap); Q9K8M4|BH2982 hypothetical protein from Bacillus halodurans (155 aa), FASTA scores: opt: 320, E(): 8e-16, (40.85% identity in 142 aa overlap); P52077|ELAA_ECOLI|B2267 protein ELAA from Escherichia coli strain K12 (153 aa), FASTA scores: opt: 269, E(): 3.8e-12, (35.7% identity in 140 aa overlap); etc. P9WFQ5 No intermediary metabolism and respiration P9WFQ5 GO:0008152,GO:0008080 P9WFQ5 NC_000962.3 Mycobrowser_v5 CDS 3208576 3209406 . - 0 Rv2899c fdhD Necessary for formate dehydrogenase activity Possible FdhD protein homolog Rv2899c, (MTCY274.30c), len: 276 aa. Possible fdhD protein homolog, highly similar to other bacterial fdhd protein homologs or formate dehydrogenase accessory proteins e.g. Q9ZBW0|FDHD_STRCO|SC4B5.08c from Streptomyces coelicolor (282 aa), FASTA scores: opt: 1032, E(): 3.6e-59, (59.0% identity in 278 aa overlap); BAB59387|TVG0258796 from Thermoplasma volcanium (279 aa), FASTA scores: opt: 536, E(): 3.4e-27, (38.65% identity in 282 aa overlap); Q9HL17|FDHD_THEAC|TA0423 from Thermoplasma acidophilum (282 aa), FASTA scores: opt: 529, E(): 9.6e-27, (38.8% identity in 281 aa overlap); P32177|FDHD_ECOLI FDHD protein from Escherichia coli strain K12 (277 aa), FASTA scores: opt: 297, E(): 8.6e-12, (33.35% identity in 261 aa overlap); etc. Contain a Pfam match to entry PF02634 FdhD/NarQ family. Belongs to the FdhD family. P9WNF1 No intermediary metabolism and respiration P64118 P9WNF1 GO:0008863,GO:0009326,GO:0005737 P9WNF1 NC_000962.3 Mycobrowser_v5 CDS 3289790 3290506 . + 0 Rv2944 Rv2944 Required for the transposition of the insertion element IS1533 Possible transposase for insertion sequence element IS1533 Rv2944, (MTCY24G1.05c), len: 238 aa. Possible transposase for IS1533, similar to is-element proteins e.g. P15026|ISTB_ECOLI istb protein from Escherichia coli (265 aa), FASTA scores: opt: 475, E (): 1.6e-21, (48.0% identity in 148 aa overlap); Z95436|MTY15C10_14 from Mycobacterium tuberculosis (248 aa), FASTA scores: opt: 784, E(): 0, (87.4% identity in 135 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). P96287 No insertion seqs and phages P96287 P96287 GO:0017111,GO:0005524 P96287 NC_000962.3 Mycobrowser_v5 CDS 3168583 3169359 . - 0 Rv2857c Rv2857c Function unknown; possibly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv2857c, (MTV003.03c), len: 258 aa. Probable short-chain dehydrogenase/reductase, highly similar to various dehydrogenases e.g. O88068|SCI35.33c probable dehydrogenase (SDR family) from Streptomyces coelicolor (260 aa), FASTA scores: opt: 1208, E(): 2e-68, (72.35% identity in 253 aa overlap); Q9I376|PA1649 from Pseudomonas aeruginosa probable short-chain dehydrogenase (253 aa), FASTA scores: opt: 569, E(): 2.1e-28, (39.2% identity in 255 aa overlap); Q9EX74|MLHA SDR-like enzyme from Rhodococcus erythropolis (246 aa), FASTA scores: opt: 567, E(): 2.8e-28, (41.15% identity in 248 aa overlap); etc. Also similar to many Mycobacterium tuberculosis dehydrogenases e.g. FABG3|Rv2002|MT2058|MTCY39.16c putative oxidoreductase (260 aa), FASTA score: (38.3% identity in 248 aa overlap). Belongs to the short-chain dehydrogenases/reductases (SDR) family. I6Y1W3 No intermediary metabolism and respiration O33339 I6Y1W3 GO:0055114,GO:0016491 1.-.-.- I6Y1W3 NC_000962.3 Mycobrowser_v5 CDS 3174992 3175372 . + 0 Rv2863 vapC23 Unknown Possible toxin VapC23 Rv2863, (MTV003.09), len: 126 aa. Possible vapC23, toxin, part of toxin-antitoxin (TA) operon with Rv2862A, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. Q50595|YI38_MYCTU|Rv1838c|MT1886|MTCY1A11.05|MTCY359.35 conserved hypothetical protein (131 aa), FASTA scores: opt: 299, E(): 6.5e-15, (39.0% identity in 123 aa overlap). P9WF89 No virulence, detoxification, adaptation O33345 P9WF89 P9WF89 NC_000962.3 Mycobrowser_v5 CDS 3177537 3177818 . + 0 Rv2865 relF Function unknown Antitoxin RelF Rv2865, (MTV003.11), len: 93 aa. RelF, antitoxin, part of toxin-antitoxin (TA) operon with Rv2866 (See Pandey and Gerdes, 2005), showing weak similarity with P58235|YR54_SYNY3|SSR2754 hypothetical 9.7 KDA protein from Synechocystis sp. strain PCC 6803 (87 aa), FASTA scores: opt: 134, E(): 0.007, (30.65% identity in 75 aa overlap); BAB58570|SAV2408 conserved hypothetical protein from Staphylococcus aureus subsp. aureus Mu50 (83 aa), FASTA scores: opt: 124, E(): 0.037, (27.5% identity in 80 aa overlap). Also similar to Rv1247|MTV006.19c hypothetical 9.8 KDA protein from Mycobacterium tuberculosis (89 aa), FASTA scores: opt: 249, E(): 2.6e-11, (44.2% identity in 86 aa overlap). O33347 No virulence, detoxification, adaptation 3G5O O33347 O33347 O33347 NC_000962.3 Mycobrowser_v5 CDS 3191644 3192201 . - 0 Rv2882c frr Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another. Ribosome recycling factor Frr (ribosome releasing factor) (RRF) Rv2882c, (MTCY274.13c), len: 185 aa. Probable frr, ribosome recycling factor, equivalent to O33046|RRF_MYCLE|FRR|ML1590|MLCB250.76 ribosome recycling factor from Mycobacterium leprae (185 aa), FASTA scores: opt: 1063, E(): 2.6e-60, (90.8% identity in 185 aa overlap). Also highly similar to others e.g. O86770|RRF_STRCO|FRR|SC6A9.40c from Streptomyces coelicolor (185 aa), FASTA scores: opt: 783, E(): 1.5e-42, (63.25% identity in 185 aa overlap); P81101|RRF_BACSU|FRR from Bacillus subtilis (184 aa), FASTA scores: opt: 640, E(): 1.7e-33, (51.65% identity in 182 aa overlap); P16174|RRF_ECOLI|FRR|B0172|Z0183|ECS0174 from Escherichia coli strains K12 and O157:H7 (185 aa), FASTA scores: opt: 473, E(): 1.4e-23, (40.2% identity in 184 aa overlap); etc. Belongs to the RRF family. P9WGY1 No information pathways 1WQF,1WQG,1WQH P66734 P9WGY1 GO:0006415,GO:0005737 P9WGY1 NC_000962.3 Mycobrowser_v5 CDS 3863317 3864531 . - 0 Rv3446c Rv3446c Unknown Hypothetical alanine and valine rich protein Rv3446c, (MTCY77.18c), len: 404 aa. Hypothetical unknown ala-, val-rich protein. O06263 No conserved hypotheticals O06263 O06263 NC_000962.3 Mycobrowser_v5 CDS 3171627 3173000 . - 0 Rv2860c glnA4 Involved in glutamine biosynthesis [catalytic activity: ATP + L-glutamate + NH(3) = ADP + glutamine + orthophosphate]. Probable glutamine synthetase GlnA4 (glutamine synthase) (GS-II) Rv2860c, (MTV003.06c), len: 457 aa. Probable glnA4, glutamine synthetase class II, similar to many glutamine synthases e.g. O88070|SCI35.35c from Streptomyces coelicolor (462 aa), FASTA scores: opt: 1947, E(): 8.2e-120, (64.15% identity in 452 aa overlap); Q98H15|MLL3074 from Rhizobium loti (Mesorhizobium loti) (465 aa), FASTA scores: opt: 1321, E(): 7.8e-79, (46.7% identity in 452 aa overlap); Q98EM0|MLL4187 from Rhizobium loti (Mesorhizobium loti) (456 aa), FASTA scores: opt: 698, E(): 4.6e-38, (33.5% identity in 454 aa overlap); Q9CDL9|GLNA from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (446 aa), FASTA scores: opt: 633, E(): 8.2e-34, (32.45% identity in 456 aa overlap); etc. Also similar to three other potential glutamine synthases in Mycobacterium tuberculosis: Q10378|GLN2_MYCTU|GLNA2|Rv2222c|MT2280|MTCY190.33c|MTCY427.03c probable glutamine synthetase (446 aa), FASTA score: (31.1% identity in 453 aa overlap); Rv1878|glnA3 and Rv2220|glnA1. Belongs to the glutamine synthetase family. I6X5K1 No intermediary metabolism and respiration O33342 I6X5K1 GO:0006542,GO:0004356 6.3.1.2 I6X5K1 NC_000962.3 Mycobrowser_v5 CDS 3173160 3174017 . - 0 Rv2861c mapB Removes the amino-terminal methionine from nascent proteins [catalytic activity: L-methionylpeptide + H(2)O = L-methionine + peptide]. Methionine aminopeptidase MapB (map) (peptidase M) Rv2861c, (MT2929, MTV003.07c), len: 285 aa. mapB (alternate gene name: map), methionine aminopeptidase, equivalent to Q9CBU7|MAPB|ML1576 methionine aminopeptidase from Mycobacterium leprae (285 aa), FASTA scores: opt: 1729, E(): 1e-99, (89.75% identity in 283 aa overlap). Also highly similar to many e.g. Q9RKR2|MAP3 from Streptomyces coelicolor (285 aa), FASTA scores: opt: 1385, E(): 2e-78, (70.65% identity in 283 aa overlap); Q9SW64|C7A10.320|AT4G37040 from Arabidopsis thaliana (Mouse-ear cress) (305 aa), FASTA scores: opt: 914, E(): 3e-49, (50.35% identity in 286 aa overlap); P07906|AMPM_ECOLI|map|B0168|Z0178|ECS0170 from Escherichia coli strains K12 and O157:H7 (264 aa), FASTA scores: opt: 793, E(): 8.5e-42, (51.0% identity in 245 aa overlap); etc. Belongs to peptidase family M24A; also known as the map family 1. Cofactor: cobalt; binds 2 ions per subunit. Note that this gene has an N-terminal extension present in the human map, but not in the prokaryotic map's. An alternative start, with RBS, will give a protein equivalent to the shorter prokaryotic map's. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P9WK19 No intermediary metabolism and respiration 1Y1N,1YJ3,3IU7,3IU8,3IU9,3PKA,3PKB,3PKC,3PKD,3PKE P0A5J2 P9WK19 GO:0009987,GO:0046872,GO:0008235,GO:0006508,GO:0004177 3.4.11.18 P9WK19 NC_000962.3 Mycobrowser_v5 CDS 3179368 3180531 . - 0 Rv2868c gcpE Unknown. GCPE is an essential gene. Probable GcpE protein Rv2868c, (MTV003.14c), len: 387 aa. Probable gcpE protein (protein e), equivalent to Q9CBU5|GCPE|ML1581 hypothetical protein GCPE from Mycobacterium leprae (392 aa), FASTA scores: opt: 2247, E(): 6.8e-134, (87.65% identity in 388 aa overlap). Highly similar to essential gene of unknown function from Escherichia coli and other prokaryotes e.g. Q9X7W2|GCPE_STRCO|SC6A5.16 GCPE protein homolog from Streptomyces coelicolor (384 aa), FASTA scores: opt: 1965, E(): 3.8e-116, (78.2% identity in 385 aa overlap); P54482|GCPE_BACSU GCPE protein homolog from Bacillus subtilis (377 aa), FASTA scores: opt: 1157, E(): 2.6e-65, (49.55% identity in 351 aa overlap); P27433|GCPE_ECOLI|B2515|Z3778|ECS3377 GCPE protein (protein E) from Escherichia coli strains K12 and O157:H7 (372 aa), FASTA scores: opt: 984, E(): 2e-54, (44.15% identity in 360 aa overlap); etc. Belongs to the GCPE family. P9WKG3 No conserved hypotheticals O33350 P9WKG3 GO:0046429,GO:0005506,GO:0055114,GO:0016114,GO:0051539 1.17.7.1 P9WKG3 NC_000962.3 Mycobrowser_v5 CDS 3181770 3183011 . - 0 Rv2870c dxr Involved in the deoxyxylulose-5-phosphate pathway (DXP) of isoprenoid biosynthesis (at the second step). Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D- erythritol 4-phosphate (MEP). Probable 1-deoxy-D-xylulose 5-phosphate reductoisomerase Dxr (DXP reductoisomerase) (1-deoxyxylulose-5-phosphate reductoisomerase) Rv2870c, (MTCY274.01c, MTV003.16c), len: 413 aa. Probable dxr, 1-deoxy-D-xylulose 5-phosphate reductoisomerase, equivalent to Q9CBU3|DXR|ML1583 1-deoxy-D-xylulose 5-phosphate reductoisomerase from Mycobacterium leprae (406 aa), FASTA scores: opt: 2145, E(): 1e-124, (84.05% identity in 395 aa overlap). Also highly similar to others e.g. Q9AJD7|DXR from Kitasatospora griseola (Streptomyces griseolosporeus) (386 aa), FASTA scores: opt: 1176, E(): 5.2e-65, (56.45% identity in 388 aa overlap); Q9KYS1|DXR_STRCO|SC5H4.18 from Streptomyces coelicolor (401 aa), FASTA scores: opt: 1079, E(): 5.1e-59, (52.25% identity in 396 aa overlap); P45568|DXR|B0173 from Escherichia coli strain K12 (398 aa), FASTA scores: opt: 120, E(): 0.032, (52.9% identity in 34 aa overlap); etc. Contains PS00133 Zinc carboxypeptidases, zinc-binding region 2 signature. Belongs to the DXR family. N-terminus shortened since first submission. P9WNS1 No intermediary metabolism and respiration 2C82,2JCV,2JCX,2JCY,2JCZ,2JD0,2JD1,2JD2,3RAS,4A03 P64012 P9WNS1 GO:0046872,GO:0055114,GO:0016114,GO:0030604 1.1.1.267 P9WNS1 NC_000962.3 Mycobrowser_v5 CDS 3183382 3183825 . + 0 Rv2872 vapC43 Unknown Possible toxin VapC43. Contains PIN domain. Rv2872, (MTCY274.03), len: 147 aa. Possible vapC43, toxin, part of toxin-antitoxin (TA) operon with Rv2871, contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. O53683|Rv0277c|MTV035.05c (142 aa), FASTA scores: opt: 357, E(): 1.4e-17, (41.45% identity in 140 aa overlap); O53812|Rv0749|MTV041.23 (142 aa), FASTA scores: opt: 350, E(): 4.3e-17, (41.55% identity in 142 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WF55 No virulence, detoxification, adaptation P65043 P9WF55 P9WF55 NC_000962.3 Mycobrowser_v5 CDS 3407314 3407688 . - 0 Rv3046c Rv3046c Function unknown Conserved protein Rv3046c, (MTV012.61c), len: 124 aa. Conserved protein, similar to several hypothetical mycobacterial proteins e.g. Q50171|ML2258 U296W hypothetical protein from Mycobacterium leprae (100 aa), FASTA scores: opt: 194, E(): 7.6e-06, (35.9% identity in 103 aa overlap); and O06409|Rv0543c|MTCY25D10.22c from Mycobacterium tuberculosis (100 aa), FASTA scores: opt: 192, E(): 1e-05, (34.7% identity in 98 aa overlap). I6YF16 No conserved hypotheticals O53292 I6YF16 I6YF16 NC_000962.3 Mycobrowser_v5 CDS 3187030 3187611 . + 0 Rv2875 mpt70 Unknown Major secreted immunogenic protein Mpt70 Rv2875, (MTCY274.06), len: 193 aa. Mpt70 (alternate gene name: mpb70), major secreted immunogenic protein MPT70 precursor (see citations below). Also similar to downstream ORF Q10790|MP83_MYCTU|MPT83|MPB83|Rv2873|MT2940|MTCY274.04 cell surface lipoprotein MPT83 precursor (lipoprotein P23) (220 aa), FASTA scores: opt: 806, E(): 1.2e-40, (70.25% identity in 185 aa overlap). Belongs to the MPT70 / MPT83 family. Generally found as a monomer; homodimer in culture fluids. P9WNF5 No cell wall and cell processes 1NYO P0A668 P9WNF5 GO:0005576 P9WNF5 NC_000962.3 Mycobrowser_v5 CDS 3187663 3187977 . + 0 Rv2876 Rv2876 Unknown Possible conserved transmembrane protein Rv2876, (MTCY274.07), len: 104 aa. Possible conserved transmembrane protein, equivalent (but longer 16 aa) to Q9CBU2|ML1584 possible conserved membrane protein from Mycobacterium leprae (84 aa), FASTA scores: opt: 444, E(): 8.3e-26, (73.85% identity in 88 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WL39 No cell wall and cell processes P9WL39 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 3188876 3189397 . - 0 Rv2878c mpt53 Not really known. Despite a weak homology to thioredoxin this cannot serve as a substrate for thioredoxin reductase. Furthermore it has no disulfide reducing activity. Soluble secreted antigen Mpt53 precursor Rv2878c, (MT2946, MTCY274.09c), len: 173 aa. Mpt53, secreted protein (contains N-terminal signal sequence) (see citations below). Shows some similarity with several disulfide bond interchange proteins e.g. P43787|THIX_HAEIN thioredoxin-like protein HI1115 from Haemophilus influenzae (167 aa), FASTA scores: opt: 200, E(): 1.4e-06, (28.9% identity in 135 aa overlap); P52237|TIPB_PSEFL thiol:disulfide interchange protein TIPB precursor (cytochrome C biogenesis protein TIPB) (178 aa), FASTA scores: opt: 184, E(): 1.8e-05, (26.3% identity in 171 aa overlap); etc. Also highly similar to O53924|DSBF|Rv1677|MTV047.12 putative lipoprotein from Mycobacterium tuberculosis (182 aa), FASTA scores: opt: 482, E(): 5.7e-26, (52.8% identity in 142 aa overlap). Could be belong to the thioredoxin family. Note that also previously known as dsbE. P9WG65 No cell wall and cell processes 1LU4 P0A618 P9WG65 GO:0005576,GO:0016491,GO:0045454 P9WG65 NC_000962.3 Mycobrowser_v5 CDS 3299971 3300570 . - 0 Rv2949c Rv2949c Catalyzes the conversion of chorismate to 4-hydroxybenzoate [catalytic activity: chorismate = 4-hydroxybenzoate + pyruvate] Chorismate pyruvate lyase Rv2949c, (MTCY349.41), len: 199 aa. Chorismate pyruvate lyase, equivalent to Q9CD83|ML0133 hypothetical protein from Mycobacterium leprae (210 aa), FASTA scores: opt: 797, E(): 7.4e-47, (62.55% identity in 195 aa overlap). Equivalent to AAK47348 from Mycobacterium tuberculosis strain CDC1551 (212 aa) but shorter 13 aa. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WIC5 No intermediary metabolism and respiration O86325 P9WIC5 GO:0016829 4.1.3.- P9WIC5 NC_000962.3 Mycobrowser_v5 CDS 3189851 3190678 . - 0 Rv2880c Rv2880c Function unknown Conserved hypothetical protein Rv2880c, (MTCY274.11c), len: 275 aa. Conserved hypothetical protein, highly similar in N-terminus to others e.g. O86754|SC6A9.22c hypothetical 40.4 KDA protein from Streptomyces coelicolor (368 aa), FASTA scores: opt: 663, E(): 2.6e-33, (52.6% identity in 213 aa overlap); Q55880|Y098_SYNY3|SLL0098 hypothetical 38.9 KDA protein from Synechocystis sp. strain PCC 6803 (350 aa), FASTA scores: opt: 362, E(): 7.3e-15, (38.9% identity in 162 aa overlap); O66732|AQ_416 hypothetical 40.2 KDA protein from Aquifex aeolicus (348 aa), FASTA scores: opt: 321, E(): 2.4e-12, (39.75% identity in 146 aa overlap); etc. Appears to be a frame shift with respect to preceding ORF but we can detect no error in the cosmid sequence to account for this. P0A644 No conserved hypotheticals P0A644 P0A644 GO:0008173,GO:0008757,GO:0005737,GO:0046872,GO:0006364,GO:0051539 2.1.1.- P9WH15 NC_000962.3 Mycobrowser_v5 CDS 3192373 3193158 . - 0 Rv2883c pyrH Uridine monophosphate kinase [catalytic activity: ATP + UMP = ADP + UDP]. Probable uridylate kinase PyrH (UK) (uridine monophosphate kinase) (UMP kinase) Rv2883c, (MT2951, MTCY274.14c), len: 261 aa. Probable pyrH, uridylate kinase, equivalent to O33045|PYRH_MYCLE|ML1591|MLCB250.75 uridylate kinase from Mycobacterium leprae (279 aa), FASTA scores: opt: 1437, E(): 3.8e-81, (85.05% identity in 274 aa overlap). Also highly similar to others e.g. O69913|PYRH from Streptomyces coelicolor (253 aa), FASTA scores: opt: 1086, E(): 1.4e-59, (68.9% identity in 251 aa overlap); P74457|PYRH_SYNY3|SLL0144 from Synechocystis sp. strain PCC 6803 (260 aa), FASTA scores: opt: 851, E(): 4.1e-45, (55.85% identity in 231 aa overlap); P29464|PYRH_ECOLI|SMBA|B0171|Z0182|ECS0173 from strains K12 and O157:H7 (240 aa), FASTA scores: opt: 666, E(): 1.1e-35, (45.7% identity in 232 aa overlap); etc. P9WHK5 No intermediary metabolism and respiration 3NWY P65929 P9WHK5 GO:0033862,GO:0008652,GO:0005737,GO:0006221,GO:0005524 2.7.4.22 P9WHK5 NC_000962.3 Mycobrowser_v5 CDS 3214628 3215572 . + 0 Rv2905 lppW Unknown Probable conserved alanine rich lipoprotein LppW Rv2905, (MTCY274.36), len: 314 aa. Probable lppW, conserved ala-rich lipoprotein, with slight similarity to beta-lactamases and hypothetical proteins e.g. Q9S1P7|SCJ9A.23 hypothetical 36.3 KDA protein from Streptomyces coelicolor (336 aa), FASTA scores: opt: 222, E(): 2.8e-06, (25.5% identity in 298 aa overlap); O69914|SC3C8.01 putative secreted protein from Streptomyces coelicolor (302 aa), FASTA scores: opt: 201, E(): 5.1e-05, (24.9% identity in 257 aa overlap); P14559|BLAC_STRAL beta-lactamase precursor from Streptomyces albus G (314 aa), FASTA scores: opt: 113, E(): 3.3, (25.2% identity in 278 aa overlap); etc. Has signal peptide and appropriately positioned prokaryotic lipoprotein lipid attachment site: attached to the membrane by a lipid anchor (potential). P9WK67 No cell wall and cell processes P9WK67 GO:0005886 P9WK67 NC_000962.3 Mycobrowser_v5 CDS 4168536 4169300 . + 0 Rv3723 Rv3723 Unknown Probable conserved transmembrane protein Rv3723, (MTV025.071), len: 254 aa. Probable conserved transmembrane protein, with hydrophobic stretches at the N-terminus, and equivalent to O69512|ML2337|MLCB2407.13c putative membrane protein from Mycobacterium leprae (250 aa), FASTA scores: opt: 1029, E(): 1.2e-44, (64.45% identity in 253 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O69690 No cell wall and cell processes O69690 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 3194166 3195548 . - 0 Rv2885c Rv2885c Required for the transposition of the insertion element IS1539. Probable transposase Rv2885c, (MTCY274.16c), len: 460 aa. Probable transposase for IS1539. Contains PS00017 ATP/GTP-binding site motif A (P-loop). P9WL37 No insertion seqs and phages Q10809 P9WL37 GO:0006310,GO:0046872,GO:0032196,GO:0003677 P9WL37 NC_000962.3 Mycobrowser_v5 CDS 3196431 3196850 . + 0 Rv2887 Rv2887 Involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv2887, (MTCY274.18), len: 139 aa. Probable transcriptional regulatory protein, highly similar to Q9EX59|SC1A4.04 putative MarR-family transcriptional regulator from Streptomyces coelicolor (151 aa), FASTA scores: opt: 354, E(): 6.6e-16, (42.95% identity in 135 aa overlap); and similar to others e.g. AAF97817|SLYA transcriptional regulator SLYA from Escherichia coli strain EPEC 2348/69 (146 aa), FASTA scores: opt: 181, E(): 0.0001, (27.25% identity in 132 aa overlap); P55740|SLYA_ECOLI|AAG56631|B1642|Z2657|ECS2351 transcriptional regulator SLYA from Escherichia coli strains K12 and O157:H7 (146 aa), FASTA scores: opt: 177, E(): 0.00018, (27.25% identity in 132 aa overlap) ; etc. Contains probable helix-turn-helix motif at aa 50-71 (Score 1182, +3.21 SD). P9WME9 No regulatory proteins P67747 P9WME9 GO:0006355,GO:0006350,GO:0003700,GO:0005622 P9WME9 NC_000962.3 Mycobrowser_v5 CDS 3196864 3198285 . - 0 Rv2888c amiC Hydrolyzes a monocarboxylic acid amide and generates a monocarboxylate [catalytic activity: a monocarboxylic acid amide + H(2)O = a monocarboxylate + NH(3)]. Probable amidase AmiC (aminohydrolase) Rv2888c, (MTCY274.19c), len: 473 aa. Probable amiC, amidase, equivalent to O33040|AMI3_MYCLE|AMIC|ML1596|MLCB250.65 putative amidase AMIC from Mycobacterium leprae (468 aa), FASTA scores: opt: 2361, E(): 4.2e-139, (76.7% identity in 468 aa overlap). Also similar to others e.g. Q9A8N0|CC1323 putative 6-aminohexanoate-cyclic-dimer hydrolase from Caulobacter crescentus (521 aa), FASTA scores: opt: 925, E(): 7.4e-50, (36.55% identity in 465 aa overlap); O28325|YJ54_ARCFU|AF1954 putative amidase from Archaeoglobus fulgidus (453 aa), FASTA scores: opt: 659, E(): 2.2e-33, (31.1% identity in 460 aa overlap); Q55424|AMID_SYNY3|SLL0828 putative amidase from Synechocystis sp. strain PCC 6803 (506 aa), FASTA scores: opt: 643, E(): 2.4e-32, (30.7% identity in 466 aa overlap); etc. Also similar to O05835|AMI1_MYCTU|AMIA2|Rv2363|MT2432|MTCY27.17c putative amidase AMIA2 (484 aa), FASTA scores: opt: 656, E(): 3.6e-33, (35.9% identity in 465 aa overlap); and Q11056|AMI2_MYCTU|AMIB2|Rv1263|MT1301|MTCY50.19c putative amidase from Mycobacterium tuberculosis (462 aa), FASTA scores: opt: 650, E(): 8.2e-33, (33.45% identity in 472 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-poop). Belongs to the amidase family. P9WQ95 No intermediary metabolism and respiration P63494 P9WQ95 GO:0016884,GO:0004040 3.5.1.4 P9WQ95 NC_000962.3 Mycobrowser_v5 CDS 3219274 3219861 . - 0 Rv2912c Rv2912c Thought to be involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably TetR-family) Rv2912c, (MTCY274.44c), len: 195 aa. Probable transcription regulatory protein, TetR family, showing similarity with others e.g. Q9K3V9|SCD10.17 putative TetR-family transcriptional from Streptomyces coelicolor (202 aa), FASTA scores: opt: 185, E(): 4.4e-05, (31.15% identity in 167 aa overlap); Q9KFQ0 TetR-family from Bacillus halodurans (185 aa), FASTA scores: opt: 164, E(): 0.001, (35.6% identity in 73 aa overlap); P17446|BETI_ECOLI|BETI|B0313 regulatory protein from Escherichia coli strain K12 (195 aa), FASTA scores: opt: 126, E(): 0.024, (24.5% identity in 196 aa overlap); etc. Contains possible helix-turn-helix motif at aa 33-54 (+2.71 SD). Possibly belongs to the TetR/AcrR family. P9WMC7 No regulatory proteins P67440 P9WMC7 GO:0006350,GO:0003700,GO:0006355 P9WMC7 NC_000962.3 Mycobrowser_v5 CDS 3251072 3255688 . + 0 Rv2932 ppsB Involved in phenolpthiocerol and phthiocerol dimycocerosate (dim) biosynthesis: extension with malony CoA (partial reduction). Phenolpthiocerol synthesis type-I polyketide synthase PpsB Rv2932, (MTV011.01, MTCY338.21, MT3002), len: 1538 aa. PpsB, type-I polyketide synthase (see citations below), highly similar to others from Mycobacterium leprae e.g. Q9S384|ML2356|MLCB12.01c putative polyketide synthase (1540 aa), FASTA scores: opt: 7284, E(): 0, (76.3% identity in 1561 aa overlap); Q49932|PKSC|L518_F1_2 putative polyketide synthase (1446 aa), FASTA scores: opt: 6811, E(): 0, (76.2% identity in 1462 aa overlap); etc. Also similar to polyketide synthases from other bacteria e.g. Q9KIZ6|EPOE EPOE protein from Polyangium cellulosum (3798 aa), FASTA scores: opt: 3052, E(): 3.3e-165, (38.35% identity in 1538 aa overlap); etc. And also highly similar to others from Mycobacterium tuberculosis e.g. Q10977|PPSA_MYCTU|RV2931 phenolpthiocerol synthesis polyketide synthase (1876 aa), FASTA scores: opt: 4227, E(): 0, (46.9% identity in 1810 aa overlap); P96203|PPSD|Rv2934|MTCY19H9.02 PKSE protein (1827 aa), FASTA scores: opt: 3756, E(): 1.8e-205, (42.9% identity in 1808 aa overlap); etc. Overlaps and extends CDS from neighbouring cosmid MTCY338.21. Contains PS00606 Beta-ketoacyl synthases active site. Note that Rv2932|ppsB belongs to the transcriptional unit Rv2930|fadD26-Rv2939|papA5 (proven experimentally). Nucleotide position 3254365 in the genome sequence has been corrected, T:C resulting in L1098L. P9WQE5 No lipid metabolism Q10978 P9WQE5 GO:0009058,GO:0048037,GO:0055114,GO:0016491,GO:0016740,GO:0000036 P9WQE5 NC_000962.3 Mycobrowser_v5 CDS 3199119 3199982 . - 0 Rv2890c rpsB Involved in translation mechanism. 30S ribosomal protein S2 RpsB Rv2890c, (MTCY274.21c), len: 287 aa. rpsB, 30s ribosomal protein s2, equivalent to O33038|RS2_MYCLE|RPSB|ML1598|MLCB250.63 30S ribosomal protein S2 from Mycobacterium leprae (277 aa), FASTA scores: opt: 1593, E(): 2.3e-93, (91.5% identity in 270 aa overlap). Also highly similar to others e.g. O31212|RS2_STRCO|RPSB|SC2E1.41 from Streptomyces coelicolor (310 aa), FASTA scores: opt: 1302, E(): 6.1e-75, (70.6% identity in 289 aa overlap); Q9KA63|RPSB|BH2427 from Bacillus halodurans (244 aa), FASTA scores: opt: 991, E(): 2.3e-55, (59.6% identity in 255 aa overlap); P21464|RS2_BACSU|RPSB from Bacillus subtilis (245 aa), FASTA scores: opt: 959, E(): 2.4e-53, (58.55% identity in 246 aa overlap); etc. Contains PS00962 Ribosomal protein S2 signature 1. Belongs to the S2P family of ribosomal proteins. P9WH39 No information pathways P66537 P9WH39 GO:0003735,GO:0006412,GO:0015935 P9WH39 NC_000962.3 Mycobrowser_v5 CDS 3200266 3201015 . + 0 Rv2891 Rv2891 Function unknown Conserved hypothetical protein Rv2891, (MTCY274.22), len: 249 aa (C-terminus overlaps neigbouring ORF). Conserved hypothetical protein, similar in N-terminus to O69910|SC2E1.40c hypothetical 22.8 KDA protein from Streptomyces coelicolor (226 aa), FASTA scores: opt: 315, E(): 3.4e-11, (40.7% identity in 145 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). P9WL33 No conserved hypotheticals P65047 P9WL33 P9WL33 NC_000962.3 Mycobrowser_v5 CDS 3200794 3202020 . - 0 Rv2892c PPE45 Function unknown PPE family protein PPE45 Rv2892c, (MTCY274.23c), len: 408 aa. PPE45, Member of the Mycobacterium tuberculosis PPE family, highly similar to many e.g. O06386|Rv3621c|MTCY15C10.31|MTCY07H7B.01 from M. tuberculosis (413 aa), FASTA scores: opt: 957, E(): 6.2e-46, (44.7% identity in 423 aa overlap). P9WHZ1 No PE/PPE P0A694 P9WHZ1 GO:0005886,GO:0016021 P9WHZ1 NC_000962.3 Mycobrowser_v5 CDS 3242198 3242983 . + 0 Rv2928 tesA Probably involved in biosynthesis of phthiocerol dimycocerosate (PDIM) Probable thioesterase TesA Rv2928, (MTCY338.17), len: 261 aa. Probable tesA, thioesterase, similar to many e.g. Q9L4W2|NYSE thioesterase involved in synthesis of the polyene antifungal antibiotic nystatin from Streptomyces noursei (see Brautaset et al., 2000) (251 aa). TesA|Rv2928 interacts with PpsE|Rv2935, by bacterial two-hybrid and GST-pulldown assays (See Rao and Ranganathan, 2004). P9WQD5 No lipid metabolism P63460 P9WQD5 GO:0016788,GO:0009058 3.1.2.- P9WQD5 NC_000962.3 Mycobrowser_v5 CDS 3318901 3319467 . - 0 Rv2966c Rv2966c Thought to cause methylation. Possible methyltransferase (methylase) Rv2966c, (MTCY349.21), len: 188 aa. Possible methyltransferase, equivalent (but shorter 36 aa) to O69465|MLCB1243.09 hypothetical 23.0 KDA protein from Mycobacterium leprae (220 aa), FASTA scores: opt: 872, E(): 9.1e-50, (74.2% identity in 182 aa overlap). Also similar to others e.g. Q9ZBR2|SC7A1.11 putative methylase from Streptomyces coelicolor (195 aa), FASTA scores: opt: 510, E(): 3.7e-26, (47.5% identity in 179 aa overlap); Q9F842 hypothetical methyltransferase (fragment) from Mycobacterium smegmatis (80 aa), FASTA scores: opt: 386, E(): 2.5e-18, (75.0% identity in 80 aa overlap); P10120|YHHF_ECOLI|YHHFZ|B3465 putative methylase from Escherichia colistrain K12 (198 aa), FASTA scores: opt: 319, E(): 1.1e-13, (35.5% identity in 183 aa overlap); etc. Contains PS00092 N-6 Adenine-specific DNA methylases signature. I6XFS7 No intermediary metabolism and respiration 3P9N P95128 I6XFS7 GO:0008168,GO:0003676,GO:0032259 2.1.1.- I6XFS7 NC_000962.3 Mycobrowser_v5 CDS 3203394 3204290 . - 0 Rv2894c xerC Participates in the site-specific recombination. Acts by catalyzing the cutting and rejoining of the recombinating DNA molecules. Acts jointly with XERD. Probable integrase/recombinase XerC Rv2894c, (MTCY274.25c), len: 298 aa. Probable xerC, integrase/recombinase, equivalent to Q9CBU0|XERC|ML1600|MLCB250.62 integrase/recombinase from Mycobacterium leprae (297 aa), FASTA scores: opt: 1624, E(): 2e-97, (85.15% identity in 296 aa overlap). Also highly similar to others integrases/recombinases (generally xerC and xerD) e.g. Q9HTS4|SSS|PA5280 site-specific recombinase from Pseudomonas aeruginosa (303 aa), FASTA scores: opt: 660, E(): 3.2e-35, (41.8% identity in 299 aa overlap); Q9HXQ6|XERD|PA3738 integrase/recombinase from Pseudomonas aeruginosa (298 aa), FASTA scores: opt: 656, E(): 5.7e-35, (40.05% identity in 297 aa overlap); Q9KCP0|BH1529 integrase/recombinase from Bacillus halodurans (299 aa), FASTA scores: opt: 645, E(): 2.9e-34, (37.35% identity in 300 aa overlap); etc. Also similar to O33200|Rv1701|MTCI125.23 integrase/recombinase from Mycobacterium tuberculosis (311 aa), FASTA scores: opt: 646, E(): 2.6e-34, (43.1% identity in 304 aa overlap). Belongs to the 'phage' integrase family. P9WF35 No insertion seqs and phages P67628 P9WF35 GO:0005737,GO:0015074,GO:0007049,GO:0051301,GO:0007059,GO:0006313,GO:0009037,GO:0003677 P9WF35 NC_000962.3 Mycobrowser_v5 CDS 3204381 3205232 . - 0 Rv2895c viuB Thought to be involved in intracellular removal of iron from iron-mycobactin complex. Mycobactin is an iron-chelating compound involved in the transport of iron from the bacterial environment into the cell cytoplasm. Possible mycobactin utilization protein ViuB Rv2895c, (MT2963, MTCY274.26c), len: 283 aa. Possible viuB, mycobactin utilization protein, highly similar to Q9RJ78|SCI41.06 hypothetical 31.5 KDA protein from Streptomyces coelicolor (280 aa), FASTA scores: opt: 639, E(): 5.1e-32, (46.3% identity in 285 aa overlap); and similar to other proteins e.g. Q9F641|MXCB protein of the biosynthetic gene cluster of the myxochelin-type iron chelator from Stigmatella aurantiaca (270 aa), FASTA scores: opt: 417, E(): 2.2e-18, (34.2% identity in 263 aa overlap); Q56646|VIUB_VIBCH|VC2210 vibriobactin utilization protein from Vibrio cholerae (271 aa), FASTA scores: opt: 395, E(): 5.1e-17, (31.0% identity in 274 aa overlap); Q56743|VIUB_VIBVU vulnibactin utilization protein V from Vibrio vulnificus (271 aa), FASTA scores: opt: 390, E(): 1e-16, (33.95% identity in 274 aa overlap); etc. Equivalent to AAK47289 from Mycobacterium tuberculosis strain CDC1551 (321 aa) but shorter 38 aa. P9WL31 No intermediary metabolism and respiration P65049 P9WL31 GO:0016491,GO:0055114 P9WL31 NC_000962.3 Mycobrowser_v5 CDS 3205265 3206434 . - 0 Rv2896c Rv2896c Function unknown Conserved hypothetical protein Rv2896c, (MTCY274.27c), len: 389 aa. Conserved hypothetical protein, similar to others proteins e.g. Q9ZJ08|FIR2 from Rhodococcus fascians (293 aa), FASTA scores: opt: 663, E(): 3.3e-32, (43.7% identity in 286 aa overlap); O69892|SC2E1.21 hypothetical 37.9 KDA protein from Streptomyces coelicolor (382 aa), FASTA scores: opt: 600, E(): 2.2e-28, (46.45% identity in 267 aa overlap); Q9JWZ4|DPRA|NMA0158 DPRA homolog from Neisseria meningitidis (serogroup A) (395 aa), FASTA scores: opt: 495, E(): 4.1e-22, (34.6% identity in 347 aa overlap); etc. Nucleotide position 3205978 in the genome sequence has been corrected, A:C resulting in S153A. P9WL29 No conserved hypotheticals Q10817 P9WL29 GO:0009294 P9WL29 NC_000962.3 Mycobrowser_v5 CDS 3211803 3212108 . - 0 Rv2901c Rv2901c Function unknown Conserved protein Rv2901c, (MTCY274.32c), len: 101 aa. Conserved protein, very equivalent to O33023|ML1610|MLCB250.41 hypothetical 12.3 KDA protein from Mycobacterium leprae (101 aa), FASTA scores: opt: 658, E(): 2.6e-43, (99.0% identity in 101 aa overlap). Also highly similar to O69889|SC2E1.18 hypothetical protein from Streptomyces coelicolor and Streptomyces lividans (102 aa), FASTA scores: opt: 515, E(): 2.2e-32, (75.0% identity in 100 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WL27 No conserved hypotheticals P65051 P9WL27 NC_000962.3 Mycobrowser_v5 CDS 3212970 3213854 . - 0 Rv2903c lepB Cleavage of N-terminal leader sequences from secreted protein precursors. Probable signal peptidase I LepB (SPASE I) (leader peptidase I). Rv2903c, (MTCY274.34c), len: 294 aa. Probable lepB, signal peptidase I (type II membrane protein) (see Braunstein & Belisle 2000), equivalent to O33021|LEP_MYCLE|ML1612|MLCB250.39 probable signal peptidase I from Mycobacterium leprae (289 aa), FASTA scores: opt: 1335, E(): 1.8e-77, (69.75% identity in 301 aa overlap). Also similar to many e.g. O86869|SIPX signal peptidase I from Streptomyces lividans (320 aa), FASTA scores: opt: 474, E(): 1e-22, (43.55% identity in 248 aa overlap); O69884|SIP1|SIPW putative signal peptidase I from Streptomyces coelicolor and Streptomyces lividans (259 aa), FASTA scores: opt: 226, E(): 5e-07, (36.0% identity in 214 aa overlap); P42668|LEP_BACLI|sip signal peptidase I from Bacillus licheniformis (186 aa), FASTA scores: opt: 218, E(): 1.3e-06, (34.5% identity in 194 aa overlap); etc. Contains PS00501 Signal peptidases I serine active site,and PS00761 Signal peptidases I signature 3. Belongs to peptidase family S26; also known as type I leader peptidase family. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P9WKA1 No cell wall and cell processes Q10789 P9WKA1 GO:0005886,GO:0006508,GO:0008236,GO:0016021 3.4.21.89 P9WKA1 NC_000962.3 Mycobrowser_v5 CDS 3414232 3414684 . - 0 Rv3052c nrdI Unknown. Probably involved in ribonucleotide reductase function. Probable NrdI protein Rv3052c, (MTCY22D7.30), len: 150 aa. Probable nrdI, equivalent to Q9CBP9|NRDI|ML1735 from Mycobacterium leprae (138 aa), FASTA scores: opt: 765, E(): 3.8e-44, (79.7% identity in 138 aa overlap), and similar to many NRDI proteins e.g. Q47415|NRDI_ECOLI|B2674 from Escherichia coli (136 aa), FASTA scores: opt: 574, E(): 1.9e-31, (62.2% identity in 135 aa overlap). Belongs to the NRDI family. P9WIZ3 No information pathways P65548 P9WIZ3 P9WIZ3 NC_000962.3 Mycobrowser_v5 CDS 3216905 3217147 . - 0 Rv2908c Rv2908c Function unknown Conserved hypothetical protein Rv2908c, (MTCY274.40c), len: 80 aa. Conserved hypothetical protein, equivalent to O33015|YT08_MYCLE from Mycobacterium leprae (80 aa), FASTA scores: opt: 492, E(): 3.1e-29, (93.75% identity in 80 aa overlap). Also highly similar to others e.g. O69880|YE09_STRCO from Streptomyces coelicolor (79 aa), FASTA scores: opt: 356, E(): 3e-19, (71.6% identity in 74 aa overlap); Q9KA12|BH2482 protein from Bacillus halodurans (76 aa), FASTA scores: opt: 220, E(): 2.9e-09, (48.6% identity in 72 aa overlap); O31738|YLQC_BACSU hypothetical 9.1 KDA protein from Bacillus subtilis (81 aa), FASTA scores: opt: 172, E(): 1e-05, (39.2% identity in 74 aa overlap); etc. Belongs to the UPF0109 family. P9WFM7 No conserved hypotheticals P9WFM7 GO:0003723 NC_000962.3 Mycobrowser_v5 CDS 3217155 3217643 . - 0 Rv2909c rpsP Involved in translation mechanism. 30S ribosomal protein S16 RpsP Rv2909c, (MTCY274.41c), len: 162 aa. rpsP, 30S ribosomal protein S16, equivalent to O33014|RS16_MYCLE 30S ribosomal protein S16 from Mycobacterium leprae (160 aa), FASTA scores: opt: 828, E(): 1.6e-39, (82.5% identity in 160 aa overlap). Also highly similar to others e.g. O69879|RS16_STRCO 30S ribosomal protein S16 from Streptomyces coelicolor (139 aa), FASTA scores: opt: 486, E(): 1.9e-20, (56.95% identity in 144 aa overlap); P80379|RS16_THETH 30S ribosomal protein S16 from Thermus Thermophilus (88 aa), FASTA scores: opt: 280, E(): 4.8e-09, (53.25% identity in 77 aa overlap) (C-terminus shorter); P21474|RS16_BACSU|RPSP 30S ribosomal protein S16 (BS17) from Bacillus subtilis (89 aa,), FASTA scores: opt: 258, E(): 8.2e-08, (42.85% identity in 91 aa overlap) (C-terminus shorter); etc. Belongs to the S16P family of ribosomal proteins. P9WH53 No information pathways P66435 P9WH53 GO:0003735,GO:0006412,GO:0005840 P9WH53 NC_000962.3 Mycobrowser_v5 CDS 3217827 3218270 . - 0 Rv2910c Rv2910c Function unknown Conserved hypothetical protein Rv2910c, (MTCY274.42c), len: 147 aa. Conserved hypothetical protein, showing some similarity with hypothetical proteins from other organisms e.g. Q9JN76|MMYY hypothetical 17.4 KDA protein from Streptomyces coelicolor (153 aa), FASTA scores: opt: 164, E(): 0.00026, (35.05% identity in 129 aa overlap); etc. Also some similarity with protein from Mycobacterium tuberculosis e.g. O07237|Rv0310c|MTCY63.15c (163 aa), FASTA scores: opt: 165, E(): 0.00023, (26.3% identity in 137 aa overlap); P96815|Rv0138|MTCI5.12 (167 aa), FASTA scores: opt: 132, E(): 0.048, (30.25% identity in 109 aa overlap); etc. P9WL25 No conserved hypotheticals P9WL25 P9WL25 NC_000962.3 Mycobrowser_v5 CDS 3223568 3224680 . - 0 Rv2915c Rv2915c Function unknown Conserved protein Rv2915c, (MTCY338.03c), len: 370 aa. Conserved protein, posssibly XAA-pro dipeptidase (prolidase), highly similar to CAC38796|SCI39.08c conserved hypothetical protein from Streptomyces coelicolor (363 aa), FASTA scores: opt: 1341, E(): 5.5e-76, (56.65% identity in 362 aa overlap); and similar to prolidases (XAA-pro dipeptidase) e.g. Q9ABC9|CC0300 putative XAA-pro dipeptidase from Caulobacter crescentus (428 aa), FASTA scores: opt: 327, E(): 7.4e-13, (30.2% identity in 374 aa overlap); Q97XD4 prolidase from Sulfolobus solfataricus (396 aa), FASTA scores: opt: 271, E(): 2.1e-09, (30.5% identity in 354 aa overlap); Q9WX55 prolidase from Microbacterium esteraromaticum (393 aa), FASTA scores: opt: 256, E(): 1.8e-08, (27.95% identity in 365 aa overlap); etc. Also similar to O53619|Rv0074|MTV030.18 conserved hypothetical protein from Mycobacterium tuberculosis (411 aa), FASTA scores: opt: 243, E(): 1.2e-07, (27.5% identity in 389 aa overlap). P9WL23 No conserved hypotheticals P68917 P9WL23 GO:0016787 P9WL23 NC_000962.3 Mycobrowser_v5 CDS 3234189 3237806 . - 0 Rv2922c smc Plays an important role in chromosome structure and partitioning. Essential for chromosome partition. Probable chromosome partition protein Smc Rv2922c, (MT2990, MTCY338.11c), len: 1205 aa. Probable smc, chromosome partition protein (ala-, arg-, leu-, glu-rich protein, possibly coiled-coil protein) (see * below), equivalent (but longer 84 aa) to Q9CBT5|SMC|ML1629|MLCB250.01 possible cell division protein from Mycobacterium leprae (1203 aa), FASTA scores: opt: 5957, E(): 0, (79.15% identity in 1205 aa overlap). Also highly similar to other chromosome segregation proteins e.g. Q9ZBQ2|SC7A1.21 putative chromosome associated protein from Streptomyces coelicolor (1186 aa), FASTA scores: opt: 2633, E(): 4.1e-120, (53.03% identity in 1205 aa overlap); P51834|SMC_BACSU chromosome partition protein from Bacillus subtilis (1186 aa), FASTA scores: opt: 1009, E(): 2.1e-41, (30.75% identity in 1205 aa overlap); Q9CHC9|SMC chromosome segregation protein from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (924 aa), FASTA scores: opt: 996, E(): 7.5e-41, (29.75% identity in 874 aa overlap); etc. Equivalent to AAK47317 from Mycobacterium tuberculosis strain CDC1551 (1205 aa) but longer 84 aa. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the SMC family. N-terminus shortened since first submission. [* Note: Unpublished. Cobbe N., Heck M.M.S.- Phylogenetic analysis of SMC proteins (OCT-2001)]. P9WGF3 No cell wall and cell processes Q10970 P9WGF3 GO:0051276,GO:0005515,GO:0005524 P9WGF3 NC_000962.3 Mycobrowser_v5 CDS 3238086 3238499 . - 0 Rv2923c Rv2923c Function unknown Conserved protein Rv2923c, (MTCY338.12c), len: 137 aa. Conserved protein, showing similarity with other hypothetical proteins e.g. P24246|YHFA_ECOLI|B3356|Z4717|ECS4207 from Escherichia coli strains K12 and O157:H7 (134 aa), FASTA scores: opt: 110, E(): 1.9, (25.9% identity in 135 aa overlap); etc. P9WL19 No conserved hypotheticals P65055 P9WL19 GO:0006950 P9WL19 NC_000962.3 Mycobrowser_v5 CDS 3600635 3600823 . + 0 Rv3224A Rv3224A Function unknown Conserved hypothetical protein Rv3224A, len: 62 aa. Conserved hypothetical protein (possibly gene fragment), overlaps Rv3224. Similar to N-terminus of ML0799|AL583919_131 conserved hypothetical protein from Mycobacterium leprae (135 aa), FASTA scores: opt: 104, E(): 0.78, (59.37% identity in 32 aa overlap). Note that upstream ORF Rv3224B is similar to C-terminus of ML0799. There appears to be no frameshift as sequence is identical in strain CDC1551 and in Mycobacterium bovis. Predicted to be an outer membrane protein (See Song et al., 2008). Q6MWZ5 No conserved hypotheticals Q6MWZ5 Q6MWZ5 NC_000962.3 Mycobrowser_v5 CDS 3221767 3223524 . - 0 Rv2914c pknI Involved in signal transduction (via phosphorylation). Thought to be involved in cell division/differentiation [catalytic activity: ATP + a protein = ADP + a phosphoprotein]. Probable transmembrane serine/threonine-protein kinase I PknI (protein kinase I) (STPK I) (phosphorylase B kinase kinase) (hydroxyalkyl-protein kinase) Rv2914c, (MTCY338.02c), len: 585 aa. Probable pknI, transmembrane serine/threonine-protein kinase (see citation below), ala-rich protein, highly similar to many in Mycobacterium tuberculosis and other bacteria e.g. Q9RLQ7|MBK putative serine/threonine protein kinase from Mycobacterium bovis BCG (291 aa), FASTA scores: opt: 376, E(): 1.1e-10, (36.95% identity in 287 aa overlap); P33973|PKN1_MYXXA serine/threonine-protein kinase from Myxococcus xanthus (693 aa), FASTA scores: opt: 286, E(): 5.4e-10, (29.9% identity in 374 aa overlap); P72003|PKNF_MYCTU|Rv1746|MT1788|MTCY28.09 probable serine/threonine-protein kinase from Mycobacterium tuberculosis (476 aa), FASTA scores: opt: 675, E(): 1.7e-24, (39.75% identity in 468 aa overlap); Q10697|PKNJ_MYCTU|Rv2088|MT2149|MTCY49.28 probable serine/threonine-protein kinase from Mycobacterium tuberculosis (589 aa), FASTA scores: opt: 574, E(): 1e-19, (34.85% identity in 479 aa overlap); etc. Equivalent to AAK47308 from Mycobacterium tuberculosis strain CDC1551 (603 aa) but shorter 18 aa. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases. P9WI69 No regulatory proteins P65730 P9WI69 GO:0006468,GO:0004674,GO:0005524 2.7.11.1 P9WI69 NC_000962.3 Mycobrowser_v5 CDS 3230738 3231076 . - 0 Rv2919c glnB In nitrogen-limiting conditions, when the ratio of GLN to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP by GLND|Rv2918c. P-II-UMP allows the deadenylylation of glutamine synthetase (gs), thus activating the enzyme. Converserly, in nitrogen excess P-II is deuridylated and promotes the adenylation of gs. P-II indirectly controls the transcription of the gs gene (GLNA: four copies in the genome). P-II prevents NR-II catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of GLNA. When P-II is uridylylated to P-II-UMP, these events are reversed. Probable nitrogen regulatory protein P-II GlnB Rv2919c, (MTCY338.08c), len: 112 aa. Probable glnB, nitrogen regulatory protein, highly similar to others e.g. Q9X705|GLNB PII protein from Corynebacterium glutamicum (Brevibacterium flavum) (112 aa), FASTA scores: opt: 531, E(): 4.5e-30, (68.75% identity in 112 aa overlap); P21193|GLNB_AZOBR nitrogen regulatory protein P-II from Azospirillum brasilense (112 aa), FASTA scores: opt: 496, E(): 1.2e-27, (60.7% identity in 112 aa overlap); P05826|GLNB_ECOLI|B2553|Z3829|ECS3419|STY2808 nitrogen regulatory protein P-II from Escherichia coli strains K12 and O157:H7 (112 aa), FASTA scores: opt: 487, E(): 5.3e-27, (61.6% identity in 112 aa overlap); etc. Contains PS00496 P-II protein urydylation site. Belongs to the P(II) protein family. P9WN31 No regulatory proteins 3BZQ,3LF0 P64249 P9WN31 GO:0000166,GO:0006808,GO:0045449,GO:0006350,GO:0030234 P9WN31 NC_000962.3 Mycobrowser_v5 CDS 3239829 3240551 . - 0 Rv2925c rnc Digests double-stranded RNA. Involved in the processing of ribosomal RNA precursors and of some mRNAs [catalytic activity: endonucleolytic cleavage to 5'-phosphomonoester]. Probable ribonuclease III Rnc (RNase III) Rv2925c, (MTCY338.14c), len: 240 aa. Probable rnc, ribonuclease III (RNase III), equivalent to O69469|RNC_MYCLE ribonuclease III from Mycobacterium leprae (238 aa). Also highly similar to other ribonucleases III e.g. Q9ZBQ7|RNC_STRCO from Streptomyces coelicolor (272 aa), FASTA scores: opt: 889, E(): 5.4e-51, (62.2% identity in 225 aa overlap) (N-terminus longer 21 aa); P51833|RNC_BACSU from Bacillus subtilis (249 aa), FASTA scores: opt: 493, E(): 5e-25, (43.25% identity in 215 aa overlap); P05797|RNC_ECOLI|RNC|B2567|Z3848|ECS3433 from Escherichia coli strain O157:H7 and K12 (226 aa), FASTA scores: opt: 459, E(): 7.9e-23, (41.8% identity in 213 aa overlap); etc. Contains PS00517 Ribonuclease III family signature. P9WH03 No information pathways 2A11 P66666 P9WH03 GO:0003725,GO:0006397,GO:0016075,GO:0006364,GO:0004525,GO:0005737 3.1.26.3 P9WH03 NC_000962.3 Mycobrowser_v5 CDS 3255685 3262251 . + 0 Rv2933 ppsC Involved in phenolpthiocerol and phthiocerol dimycocerosate (dim) biosynthesis: extension with malony CoA (complete reduction). Phenolpthiocerol synthesis type-I polyketide synthase PpsC Rv2933, (MTCY19H9.01, MTV011.02), len: 2188 aa. ppsC, type-I polyketide synthase (see citations below), highly similar to others from Mycobacterium leprae e.g. Q49933|PKSD|ML2355|L518_F1_3 putative polyketide synthase (2201 aa), FASTA scores: opt: 6973, E(): 0, (82.32% identity in 2217 aa overlap); Q49624|PKS3|MASA|ML1229|B1170_C2_209 probable mycocerosic acid synthase (2118 aa), FASTA scores: opt: 4015, E(): 2.9e-208, (36.6% identity in 2184 aa overlap); etc. Also similar to polyketide synthases from other bacteria e.g. C-terminus of Q9L8C7 polyketide synthase from Polyangium cellulosum (7257 aa), FASTA scores: opt: 3909, E(): 3.6e-202, (40.15% identity in 2220 aa overlap); Q9KIZ7|EPOD EPOD protein from Polyangium cellulosum (7257 aa), FASTA scores: opt: 3886, E(): 6.2e-201, (40.05% identity in 2220 aa overlap); etc. And also highly similar to others from Mycobacterium tuberculosis e.g. P96291|Rv2940c (2111 aa), FASTA scores: opt: 4204, E(): 0, (39.1% identity in 2176 aa overlap); Q10977|PPSA_MYCTU|RV2931 phenolpthiocerol synthesis polyketide synthase (1876 aa), FASTA scores: opt: 3793, E(): 2.4e-196, (46.65% identity in 1612 aa overlap); etc. Contains PS00606 Beta-ketoacyl synthases active site, and PS00012 Phosphopantetheine attachment site. Note that Rv2933|ppsC belongs to the transcriptional unit Rv2930|fadD26-Rv2939|papA5 (proven experimentally). P96202 No lipid metabolism 1PQW P96202 P96202 GO:0009058,GO:0048037,GO:0055114,GO:0016491,GO:0031177,GO:0016740,GO:0008270,GO:0000036 P96202 NC_000962.3 Mycobrowser_v5 CDS 3262248 3267731 . + 0 Rv2934 ppsD Involved in phenolpthiocerol and phthiocerol dimycocerosate (dim) biosynthesis: extension with methylmalony CoA (partial reduction). Phenolpthiocerol synthesis type-I polyketide synthase PpsD Rv2934, (MTCY19H9.02), len: 1827 aa. PpsD, type-I polyketide synthase (see citations below), highly similar to others from Mycobacterium leprae e.g. Q9CB70|ML2354 polyketide synthase (1822 aa), FASTA scores: opt: 9779, E(): 0, (80.35% identity in 1836 aa overlap); Q49940|L518_F3_67|PFSE (1815 aa), FASTA scores: opt: 9658, E(): 0, (79.85% identity in 1831 aa overlap); etc. Also similar to polyketide synthases from other bacteria e.g. C-terminus of Q9RNB2|MCYD|Q9FDU1 polyketide synthase (MCYD protein) from Microcystis aeruginosa (3906 aa), FASTA scores: opt: 2961, E(): 6e-159, (32.15% identity in 1827 aa overlap); etc. And also highly similar to others from Mycobacterium tuberculosis e.g. Q10978|PPSB_MYCTU|RV2932 phenolpthiocerol synthesis polyketide synthase (1538 aa), FASTA scores: opt: 3756, E(): 3.8e-204, (42.85% identity in 1808 aa overlap) (gaps in middle); P96202|PPSC|RV2933 polyketide synthase (2188 aa), FASTA scores: opt: 3463, E(): 1.7e-187, (39.2% identity in 2165 aa overlap); etc. Contains PS00606 Beta-ketoacyl synthases active site, PS00017 ATP/GTP-binding site motif A, PS00013 Prokaryotic membrane lipoprotein lipid attachment site, and PS00012 Phosphopantetheine attachment site. Note that Rv2934|ppsD belongs to the transcriptional unit Rv2930|fadD26-Rv2939|papA5 (proven experimentally). P9WQE3 No lipid metabolism P96203 P9WQE3 GO:0009058,GO:0048037,GO:0016491,GO:0031177,GO:0016740,GO:0000036 P9WQE3 NC_000962.3 Mycobrowser_v5 CDS 3237818 3238099 . - 0 Rv2922A acyP Involved in cellular metabolism [catalytic activity: an acylphosphate + H(2)O = a fatty acid anion + orthophosphate]. Probable acylphosphatase AcyP (acylphosphate phosphohydrolase) Rv2922A, len: 93 aa. Probable acyP, acylphosphatase (acylphosphate phosphohydrolase), highly similar to others e.g. Q9ZBQ3|SC7A1.20 putative acylphosphatase from Streptomyces coelicolor (93 aa), FASTA scores: opt: 345, E(): 9.5e-19, (58.9% identity in 90 aa overlap); P75877|ACYP_ECOLI|YCCX|B0968|Z1320|ECS1052 putative acylphosphatase from Escherichia coli strains K12 and O157:H7 (92 aa), FASTA scores: opt: 220, E(): 2e-09, (44.95% identity in 89 aa overlap); Q9RVU3|DR0929 putative acylphosphatase from Deinococcus radiodurans (87 aa), FASTA scores: opt: 193, E(): 2.1e-07, (44.3% identity in 79 aa overlap); etc. Belongs to the acylphosphatase family. P69419 No intermediary metabolism and respiration P69419 P69419 GO:0003998 3.6.1.7 NC_000962.3 Mycobrowser_v5 CDS 3238601 3239470 . - 0 Rv2924c fpg Involved in base excision repair (repair of oxidized purines). This enzyme may play a significant role in processes leading to recovery from mutagenesis and/or cell death by alkylating agents [catalytic activity hydrolysis of DNA containing ring-opened N7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimide]. Probable formamidopyrimidine-DNA glycosylase Fpg (FAPY-DNA glycosylase) Rv2924c, (MTCY338.13c), len: 289 aa. Probable fpg (alternate gene name: mutM), formamidopyrimidine-DNA glycosylase (see citation below), equivalent to O69470|FPG_MYCLE formamidopyrimidine-DNA glycosylase from Mycobacterium leprae (282 aa), FASTA scores: opt: 1563, E(): 1.3e-96, (80.6% identity in 289 aa overlap). Also highly similar to other formamidopyrimidine-DNA glycosylases e.g. Q9ZBQ6|FPG_STRCO from Streptomyces coelicolor (286 aa), FASTA scores: opt: 1047, E(): 2.9e-62, (57.55% identity in 292 aa overlap); P95744|FPG_SYNEN from Synechococcus elongatus naegeli (284 aa), FASTA scores: opt: 569, E(): 1.9e-30, (37.95% identity in 290 aa overlap); P05523|FPG_ECOLI|MUTM|FPG|B3635 from Escherichia coli strain K12 (269 aa), FASTA scores: opt: 424, E(): 8.2e-21, (33.9% identity in 289 aa overlap); etc. Belongs to the FPG family. Cofactor: binds 1 zinc ion. P9WNC3 No information pathways P64150 P9WNC3 GO:0003684,GO:0006289,GO:0008534,GO:0008270,GO:0006284 4.2.99.18,3.2.2.23 P9WNC3 NC_000962.3 Mycobrowser_v5 CDS 3240548 3241171 . - 0 Rv2926c Rv2926c Function unknown Conserved protein Rv2926c, (MTCY338.15c), len: 207 aa. Conserved protein, equivalent to O69468|ML1660|MLCB1243.14 hypothetical 23.5 KDA protein from Mycobacterium leprae (217 aa), FASTA scores: opt: 866, E(): 1.4e-48, (67.2% identity in 192 aa overlap). Also similar in part to other hypothetical proteins e.g. Q9WXZ8 conserved hypothetical protein from Thermotoga maritima (182 aa), FASTA scores: opt: 254, E(): 3.4e-09, (31.45% identity in 143 aa overlap); Q9ZBQ9|SC7A1.14 hypothetical 23.5 KDA protein from Streptomyces coelicolor (217 aa), FASTA scores: opt: 244, E(): 1.7e-08, (45.5% identity in 189 aa overlap); O65982 hypothetical 26.2 KDA protein from Clostridium thermosaccharolyticum (Thermoanaerobacterium thermosaccharolyticum) (228 aa), FASTA scores: opt: 220, E(): 6.1e-07, (32.45% identity in 148 aa overlap); etc. Equivalent to AAK47323 from Mycobacterium tuberculosis strain CDC1551 (195 aa) but longer 12 aa. P9WL17 No conserved hypotheticals P65057 P9WL17 NC_000962.3 Mycobrowser_v5 CDS 3242970 3243281 . + 0 Rv2929 Rv2929 Unknown Hypothetical protein Rv2929, (MTCY338.18), len: 103 aa. Hypothetical unknown protein; some weak similarity to C-terminal half of P18319|UREG_KLEAE urease accessory protein from klebsiella aerogenes (205 aa), FASTA scores: opt: 99, E(): 1.1, (38.6% identity in 57 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WL13 No conserved hypotheticals P9WL13 NC_000962.3 Mycobrowser_v5 CDS 3243697 3245448 . + 0 Rv2930 fadD26 Involved in phthiocerol dimycocerosate (dim) biosynthesis, possibly by activating substrates for the PPS polyketides synthase. Fatty-acid-AMP ligase FadD26 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) Rv2930, (MT2999, MTCY338.19), len: 583 aa. FadD26, fatty-acid-AMP synthetase, equivalent to Q9Z5K5|FADD26|ML2358|MLCB12.03c probable acyl-CoA synthase from Mycobacterium leprae (583 aa), FASTA scores: opt: 3026, E(): 9.2e-180, (76.85% identity in 583 aa overlap). Also highly similar to many e.g. Q9CD84|ML0132 putative acyl-CoA synthetase from Mycobacterium leprae (680 aa), FASTA scores: opt: 2324, E(): 3.2e-136, (61.35% identity in 572 aa overlap); P71495 acyl-CoA synthase from Mycobacterium bovis (582 aa), FASTA scores: opt: 2304, E(): 5e-135, (59.85% identity in 583 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. Q50586|FD25_MYCTU|RV1521|MTCY19G5.07 putative fatty-acid--CoA ligase (583 aa), FASTA scores: opt: 2188, E(): 7.6e-128, (57.55% identity in 584 aa overlap); etc. Belongs to the ATP-dependent AMP-binding enzyme family. N-terminus shortened since first submission. Note that Rv2930|fadD26 belongs to the transcriptional unit Rv2930|fadD26-Rv2939|papA5 (proven experimentally). P9WQ43 No lipid metabolism Q10976 P9WQ43 GO:0016874,GO:0006631 6.2.1.- P9WQ43 NC_000962.3 Mycobrowser_v5 CDS 3245445 3251075 . + 0 Rv2931 ppsA Involved in phenolpthiocerol and phthiocerol dimycocerosate (dim) biosynthesis: extension of C18 with malony CoA (partial reduction). Phenolpthiocerol synthesis type-I polyketide synthase PpsA Rv2931, (MTCY338.20), len: 1876 aa. PpsA, type-I polyketide synthase (see citations below), highly similar to others from Mycobacterium leprae e.g. Q9Z5K6|ML2357|MLCB12.02c putative polyketide synthase from Mycobacterium leprae (1871 aa), FASTA scores: opt: 7566, E(): 0, (76.1% identity in 1888 aa overlap); Q9S384|ML2356|MLCB12.01c putative polyketide synthase from Mycobacterium leprae (1540 aa), FASTA scores: opt: 4026, E(): 9.8e-212, (45.7% identity in 1811 aa overlap); Q49932|PKSC|L518_F1_2 putative polyketide synthase (1446 aa), FASTA scores: opt: 4026, E(): 9.4e-212, (70.6% identity in 885 aa overlap). Also similar to polyketide synthases from other bacteria e.g. C-terminus of Q9L8C7|EPOC polyketide synthase from Polyangium cellulosum (7257 aa), FASTA scores: opt: 2592, E(): 5.2e-133, (32.55% identity in 2245 aa overlap); P22367|MSAS_PENPA 6-methylsalicylic acid synthase from Penicillium patulum (Penicillium griseofulvum) (1774 aa), FASTA scores: opt: 2391, E(): 0, (34.2% identity in 1815 aa overlap); etc. And also highly similar to others from Mycobacterium tuberculosis e.g. Q10978|PPSB_MYCTU|RV2932 phenolpthiocerol synthesis polyketide synthase (1538 aa), FASTA scores: opt: 4227, E(): 0, (46.8% identity in 1810 aa overlap) (gap in middle); etc. Contains PS00606 Beta-ketoacyl synthases active site, and PS00012 Phosphopantetheine attachment site. Note that Rv2931|ppsA belongs to the transcriptional unit Rv2930|fadD26-Rv2939|papA5 (proven experimentally). P9WQE7 No lipid metabolism Q10977 P9WQE7 GO:0009058,GO:0048037,GO:0055114,GO:0016491,GO:0031177,GO:0016740,GO:0000036 P9WQE7 NC_000962.3 Mycobrowser_v5 CDS 3273206 3274075 . + 0 Rv2937 drrB Probably involved in active transport of antibiotic and phthiocerol dimycocerosate (dim) across the membrane (export). DRRA|Rv2934|MTCY19H9.04, DRRB and DRRC|Rv2938|MTCY19H9.06 may act jointly to confer daunorubicin and doxorubicin resistance by an export mechanism. Probably responsible for the translocation of the substrate across the membrane and localization of dim into the cell wall. Daunorubicin-dim-transport integral membrane protein ABC transporter DrrB Rv2937, (MTCY19H9.05), len: 289 aa. drrB, daunorubicin-dim-transport integral membrane protein ABC transporter, probably involved in daunorubicin resistance and phthiocerol dimycocerosate transport (see citations below), equivalent to Q49935|DRRB|ML2351|L518_F1_9 daunorubicin resistance transmembrane protein from Mycobacterium leprae (288 aa), FASTA scores: opt: 1252, E(): 5.3e-72, (64.0% identity in 289 aa overlap). Also similar to others e.g. Q9XCF8 DRRB protein from Mycobacterium avium (246 aa), FASTA scores: opt: 423, E(): 1.5e-19, (30.85% identity in 243 aa overlap); Q9S6H4 daunorubicin resistance protein B from Mycobacterium avium (246 aa), FASTA scores: opt: 420, E(): 2.3e-19, (30.85% identity in 243 aa overlap); P32011|DRRB_STRPE daunorubicin resistance transmembrane protein from Streptomyces peucetius (283 aa), FASTA scores: opt: 242, E(): 4.7e-08, (27.85% identity in 219 aa overlap); etc. Note that Rv293|drrB belongs to the transcriptional unit Rv2930|fadD26-Rv2939|papA5 (proven experimentally). P9WG23 No cell wall and cell processes P9WG23 GO:0005886,GO:0046677,GO:0055085,GO:0016021 P9WG23 NC_000962.3 Mycobrowser_v5 CDS 3288464 3289705 . + 0 Rv2943 Rv2943 Required for the transposition of the insertion element IS1533. Probable transposase for insertion sequence element IS1533 Rv2943, (MTCY24G1.06c), len: 413 aa. Probable transposase for insertion sequence IS1533, similar to other transposases e.g. P15025|ISTA_ECOLI ista protein (insertion sequence IS21) from Escherichia coli (390 aa), FASTA scores: opt: 268, E(): 5.1e-11, (24.1% identity in 378 aa overlap). Contains potential helix-turn-helix motif at aa 19-40 (Score 1611, +4.67 SD). I6X5T4 No insertion seqs and phages P96288 I6X5T4 GO:0006355,GO:0016987,GO:0003700,GO:0006352,GO:0015074 I6X5T4 NC_000962.3 Mycobrowser_v5 CDS 3274949 3276217 . + 0 Rv2939 papA5 Thought to be involved in phthiocerol dimycocerosate (dim) biosynthesis. Possible conserved polyketide synthase associated protein PapA5 Rv2939, (MTCY19H9.07), len: 422 aa. Possible papA5, conserved polyketide synthase (PKS) associated protein (see Camacho et al., 2001), equivalent to Q49939 hypothetical 45.6 KDA protein from Mycobacterium leprae (423 aa), FASTA scores: opt: 2398, E(): 4.5e-144, (84.05% identity in 426 aa overlap); and Q02279|YMA3_MYCBO hypothetical 38.1 KDA protein from Mycobacterium bovis (354 aa), FASTA scores: opt: 2193, E(): 3.6e-131, (97.4% identity in 343 aa overlap). And C-terminus highly similar to to Q9S381 hypothetical 5.0 KDA protein (fragment) from Mycobacterium leprae (44 aa), FASTA scores: opt: 275, E(): 1.4e-10, (88.65% identity in 44 aa overlap). Also similar in part to various synthetases e.g. Q9AE01|RIF20 RIF20 protein from Amycolatopsis mediterranei (Nocardia mediterranei) (403 aa), FASTA scores: opt: 282, E(): 2.7e-10, (30.3% identity in 393 aa overlap); middle part of Q00869|ESYN1 enniatin sythetase (fragment) (N-methyl peptide synthetase) from Fusarium equiseti (3131 aa), FASTA scores: opt: 180, E(): 0.0036, (26.85% identity in 242 aa overlap); N-terminus of Q9FB18 peptide synthetase NRPS2-1 from Streptomyces verticillus (2626 aa), FASTA scores: opt: 159, E(): 0.068, (23.65% identity in 351 aa overlap); etc. Note that Rv2939|papA5 belongs to the transcriptional unit Rv2930|fadD26-Rv2939|papA5 (proven experimentally). P9WIN5 No lipid metabolism 1Q9J P96208 P9WIN5 GO:0008610,GO:0008415 2.3.1.- P9WIN5 NC_000962.3 Mycobrowser_v5 CDS 3276380 3282715 . - 0 Rv2940c mas Catalyzes the elongation of N-fatty acyl-CoA with methylamalonyl-CoA (not malonyl-CoA) as the elongating agent to form mycocerosyl lipids. Probable multifunctional mycocerosic acid synthase membrane-associated Mas Rv2940c, (MTCY24G1.09, MTCY19H9.08c), len: 2111 aa. Probable mas, mycocerosic acid synthase membrane associated, multifunctional enzyme (see citations below), almost identical to Q02251|MCAS_MYCBO|mas mycocerosic acid synthase from Mycobacterium bovis (2110 aa), FASTA scores: opt: 13226, E(): 0, (95.8% identity in 2115 aa overlap) (see Mathur & Kolattukudy 1992); and equivalent to Q9CD78|mas|ML0139 putative mycocerosic synthase from Mycobacterium leprae (2116 aa), FASTA scores: opt: 12142, E(): 0, (87.95% identity in 2119 aa overlap); and Q49624|PKS3|MASA|ML1229|B1170_C2_209 probable mycocerosic acid synthase from Mycobacterium leprae (2118 aa), FASTA scores: opt: 8421, E(): 0, (60.8% identity in 2127 aa overlap). Also similar to other synthases e.g. C-terminus of Q9L8C7|EPOC polyketide synthase from Polyangium cellulosum (7257 aa), FASTA scores: opt: 4332, E(): 0, (40.85% identity in 2149 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. O53901|PKS5|Rv1527c|MTV045.01c|MTCY19G5.01 polyketide synthase (2108 aa), FASTA scores: opt: 5059, E(): 0, (65.9% identity in 2121 aa overlap); etc. Contains several domains, organized in the following order: beta-ketoacyl synthase (PS00606), acyl transferase, dehydratase-enoyl reductase, beta-ketoreductase, acyl carrier protein. Contains PS00012 Phosphopantetheine attachment site. I6Y231 No lipid metabolism P96291 I6Y231 GO:0009058,GO:0048037,GO:0055114,GO:0016491,GO:0031177,GO:0016740,GO:0008270,GO:0000036 I6Y231 NC_000962.3 Mycobrowser_v5 promoter 3282902 3282907 . - 0 Rv2940c mas Catalyzes the elongation of N-fatty acyl-CoA with methylamalonyl-CoA (not malonyl-CoA) as the elongating agent to form mycocerosyl lipids. Probable multifunctional mycocerosic acid synthase membrane-associated Mas Rv2940c, (MTCY24G1.09, MTCY19H9.08c), len: 2111 aa. Probable mas, mycocerosic acid synthase membrane associated, multifunctional enzyme (see citations below), almost identical to Q02251|MCAS_MYCBO|mas mycocerosic acid synthase from Mycobacterium bovis (2110 aa), FASTA scores: opt: 13226, E(): 0, (95.8% identity in 2115 aa overlap) (see Mathur & Kolattukudy 1992); and equivalent to Q9CD78|mas|ML0139 putative mycocerosic synthase from Mycobacterium leprae (2116 aa), FASTA scores: opt: 12142, E(): 0, (87.95% identity in 2119 aa overlap); and Q49624|PKS3|MASA|ML1229|B1170_C2_209 probable mycocerosic acid synthase from Mycobacterium leprae (2118 aa), FASTA scores: opt: 8421, E(): 0, (60.8% identity in 2127 aa overlap). Also similar to other synthases e.g. C-terminus of Q9L8C7|EPOC polyketide synthase from Polyangium cellulosum (7257 aa), FASTA scores: opt: 4332, E(): 0, (40.85% identity in 2149 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. O53901|PKS5|Rv1527c|MTV045.01c|MTCY19G5.01 polyketide synthase (2108 aa), FASTA scores: opt: 5059, E(): 0, (65.9% identity in 2121 aa overlap); etc. Contains several domains, organized in the following order: beta-ketoacyl synthase (PS00606), acyl transferase, dehydratase-enoyl reductase, beta-ketoreductase, acyl carrier protein. Contains PS00012 Phosphopantetheine attachment site. I6Y231 No lipid metabolism P96291 I6Y231 GO:0009058,GO:0048037,GO:0055114,GO:0016491,GO:0031177,GO:0016740,GO:0008270,GO:0000036 I6Y231 NC_000962.3 Mycobrowser_v5 promoter 3282905 3282921 . - 0 Rv2940c mas Catalyzes the elongation of N-fatty acyl-CoA with methylamalonyl-CoA (not malonyl-CoA) as the elongating agent to form mycocerosyl lipids. Probable multifunctional mycocerosic acid synthase membrane-associated Mas Rv2940c, (MTCY24G1.09, MTCY19H9.08c), len: 2111 aa. Probable mas, mycocerosic acid synthase membrane associated, multifunctional enzyme (see citations below), almost identical to Q02251|MCAS_MYCBO|mas mycocerosic acid synthase from Mycobacterium bovis (2110 aa), FASTA scores: opt: 13226, E(): 0, (95.8% identity in 2115 aa overlap) (see Mathur & Kolattukudy 1992); and equivalent to Q9CD78|mas|ML0139 putative mycocerosic synthase from Mycobacterium leprae (2116 aa), FASTA scores: opt: 12142, E(): 0, (87.95% identity in 2119 aa overlap); and Q49624|PKS3|MASA|ML1229|B1170_C2_209 probable mycocerosic acid synthase from Mycobacterium leprae (2118 aa), FASTA scores: opt: 8421, E(): 0, (60.8% identity in 2127 aa overlap). Also similar to other synthases e.g. C-terminus of Q9L8C7|EPOC polyketide synthase from Polyangium cellulosum (7257 aa), FASTA scores: opt: 4332, E(): 0, (40.85% identity in 2149 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. O53901|PKS5|Rv1527c|MTV045.01c|MTCY19G5.01 polyketide synthase (2108 aa), FASTA scores: opt: 5059, E(): 0, (65.9% identity in 2121 aa overlap); etc. Contains several domains, organized in the following order: beta-ketoacyl synthase (PS00606), acyl transferase, dehydratase-enoyl reductase, beta-ketoreductase, acyl carrier protein. Contains PS00012 Phosphopantetheine attachment site. I6Y231 No lipid metabolism P96291 I6Y231 GO:0009058,GO:0048037,GO:0055114,GO:0016491,GO:0031177,GO:0016740,GO:0008270,GO:0000036 I6Y231 NC_000962.3 Mycobrowser_v5 CDS 3305279 3306535 . + 0 Rv2953 Rv2953 May be involved in phenolpthiocerol and phthiocerol dimycocerosate (dim) biosynthesis Enoyl reductase Rv2953, (MTCY349.37c), len: 418 aa. Enoyl reductase, equivalent to Q9CD87|ML0129 hypothetical protein from Mycobacterium leprae (418 aa), FASTA scores: opt: 2357, E(): 2.7e-143, (86.6% identity in 418 aa overlap). Also highly similar to Q9X7N5|SC5F2A.12c conserved hypothetical protein from Streptomyces coelicolor (396 aa), FASTA scores: opt: 491, E(): 7e-24, (38.35% identity in 417 aa overlap); and similar to other hypothetical proteins e.g. Q9VG81 CG5167 protein from Drosophila melanogaster (Fruit fly) (431 aa), FASTA scores: opt: 393, E(): 1.4e-17, (26.55% identity in 433 aa overlap); Q9GZE9|F22F7.1 hypothetical protein from Caenorhabditis elegans (426 aa), FASTA scores: opt: 338, E(): 4.6e-14, (27.05% identity in 425 aa overlap); P73855|SLL1601 hypothetical 44.8 KDA protein from Synechocystis sp. (strain PCC 6803) (414 aa), FASTA scores: opt: 565, E(): 1.3e-28, (35.7% identity in 409 aa overlap); etc. Also highly similar to other proteins from Mycobacterium tuberculosis e.g. RV2449C|O53176|MTV008.05C hypothetical 44.4 KDA protein (419 aa), FASTA scores: opt: 1835, E(): 7e-110, (67.55% identity in 419 aa overlap); etc. P9WGV5 No lipid metabolism P95139 P9WGV5 GO:0008610,GO:0055114,GO:0016491 1.3.1.- P9WGV5 NC_000962.3 Mycobrowser_v5 CDS 3323071 3323703 . - 0 Rv2968c Rv2968c Unknown Probable conserved integral membrane protein Rv2968c, (MTCY349.19), len: 210 aa. Probable conserved integral membrane protein, equivalent to O69464 putative integral membrane protein from Mycobacterium leprae (214 aa), FASTA scores: opt: 1060, E(): 1.4e-58, (71.95% identity in 214 aa overlap). Also highly similar to others e.g. Q9F844 hypothetical integral membrane protein from Mycobacterium smegmatis (187 aa), FASTA scores: opt: 883, E(): 1.2e-47, (62.8% identity in 190 aa overlap); Q9KXP3 putative integral membrane protein from Streptomyces coelicolor (240 aa), FASTA scores: opt: 503, E(): 4.6e-24, (38.0% identity in 192 aa overlap); etc. I6X5W1 No cell wall and cell processes P95126 I6X5W1 I6X5W1 NC_000962.3 Mycobrowser_v5 CDS 3285070 3287832 . + 0 Rv2942 mmpL7 Involved in translocation of phthiocerol dimycocerosate (dim) in the cell wall. Conserved transmembrane transport protein MmpL7 Rv2942, (MTCY24G1.07c), len: 920 aa. MmpL7, conserved transmembrane transport protein (see citations below), member of RND superfamily, highly similar to Q9XB10 hypothetical 99.5 KDA protein from Mycobacterium bovis BCG (945 aa), FASTA scores: opt: 488, E(): 4.9e-20, (29.5% identity in 918 aa overlap); and to others from Mycobacteria e.g. O53735|MML4_MYCTU from Mycobacterium tuberculosis (945 aa), FASTA scores: opt: 481, E(): 1.2e-19, (25.9% identity in 922 aa overlap); etc. Also similar to other membrane proteins e.g. O54101|MMLB_STRCO|SC10A5.10c putative membrane protein from Streptomyces coelicolor (847 aa), FASTA scores: opt: 256, E(): 7.2e-07, (25.15% identity in 545 aa overlap); etc. Contains PS00639 Eukaryotic thiol (cysteine) proteases histidine active site, PS00079 Multicopper oxidases signature 1, and PS00044 Bacterial regulatory proteins, lysR family signature. Belongs to the MmpL family. Note that Rv2941|fadD28 and Rv2942|mmpL7 are transcriptionally coupled (proven experimentally). P9WJU7 No cell wall and cell processes P65370 P9WJU7 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 3291503 3296353 . - 0 Rv2946c pks1 Polyketide synthase possibly involved in lipid synthesis Probable polyketide synthase Pks1 Rv2946c, (MTCY24G1.03), len: 1616 aa. Probable pks1, polyketide synthase, similar to many e.g. ML035|AL583917|Q9CD81 putative polyketide synthase from Mycobacterium leprae (2103 aa), Fasta scores: opt: 8761, E(): 0, (82.6% identity in 1620 aa overlap); etc. Almost identical in part to G560507|Q50470 PKS002C protein from Mycobacterium tuberculosis (fragment) (950 aa), Fasta scores: opt: 5685, E(): 0, (95.3% identity in 927 aa overlap). Also similar to Mycobacterium tuberculosis polyketide synthases pks7|Rv1661|P94996 (2126 aa) (54.6% identity in 1632 aa); pks12|Rv2048c|O53490 (4151 aa) (58.0% identity in 1606 aa); pks8|rv1662|O65933 (1602 aa) (59.7% identity in 1144 aa). Contains a PS00012 Phosphopantetheine attachment site. Note pks1 has been shown to be involved in the biosynthesis of phthiocerol. pks15/pks1 has been shown to be involved in the biosynthesis of phenolphthiocerol glycolipids. P96285 No lipid metabolism P96285 P96285 GO:0009058,GO:0048037,GO:0055114,GO:0016491,GO:0031177,GO:0016740,GO:0008270,GO:0000036 P96285 NC_000962.3 Mycobrowser_v5 CDS 3296350 3297840 . - 0 Rv2947c pks15 Polyketide synthase possibly involved in lipid synthesis Probable polyketide synthase Pks15 Rv2947c, (MTCY24G1.02), len: 496 aa. Probable pks15, polyketide synthase. Almost identical to G560508|Q50469 PKS002B protein from Mycobacterium tuberculosis (495 aa), FASTA scores: opt: 3270, E(): 0, (99.6% identity in 496 a a overlap). Similar to Mycobacterium tuberculosis proteins MTCY338.20|RV2931|PPSA_MYCTU ppsA phenolpthiocerol synthesis (1876 aa) (49.9% identity in 465 aa overlap); MTCY24G1.09|RV2940C|P96291 Putative mas, mycocerosic acid synthase (2111 aa) (50.2% identity in 454 aa overlap); and MTCY22H8.03|RV2382C|P71718 hypothetical protein (444 aa) (47.6% identity in 437 aa overlap). Contains PS00606 Beta-ketoacyl synthases active site. Note pks15 has been shown to be involved in the biosynthesis of phthiocerol. pks15/pks1 has been shown to be involved in the biosynthesis of phenolphthiocerol glycolipids. P96284 No lipid metabolism P96284 P96284 GO:0048037,GO:0016740,GO:0009058 P96284 NC_000962.3 Mycobrowser_v5 CDS 3303103 3304248 . - 0 Rv2951c Rv2951c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv2951c, (MTCY349.39), len: 381 aa. Possible oxidoreductase, equivalent to Q9CD85 putative oxidoreductase from Mycobacterium leprae (382 aa), FASTA scores: opt: 2225, E(): 7.6e-134, (84.8% identity in 382 aa overlap); and similar to O30260 conserved hypothetical protein from Mycobacterium leprae (363 aa), FASTA scores: opt: 652, E(): 6.1e-34, (32.55% identity in 344 aa overlap). Also similar to various oxidoreductases e.g. O29071|AF1196 N5,N10-methylenetetrahydromethanopterin reductase from Archaeoglobus fulgidus (348 aa), FASTA scores: opt: 381, E(): 9.7e-17, (27.7% identity in 354 aa overlap); Q58929|mer|MJ1534 F420-dependent methylenetetrahydromethanopterin reductase from Methanococcus jannaschii (331 aa), FASTA scores: opt: 372, E(): 3.5e-16, (30.85% identity in 295 aa overlap); Q9UXP0 putative F420-dependent N5,N10-methylene-tetrahydromethanopterin reductase from Methanolobus tindarius (326 aa), FASTA scores: opt: 343, E(): 2.4e-14, (27.4% identity in 314 aa overlap); etc. P9WIB7 No intermediary metabolism and respiration P95140 P9WIB7 GO:0055114,GO:0016491,GO:0008610 1.2.-.- P9WIB7 NC_000962.3 Mycobrowser_v5 CDS 3306666 3307391 . - 0 Rv2954c Rv2954c Unknown Hypothetical protein Rv2954c, (MTCY349.36), len: 241 aa. Hypothetical unknown protein. Equivalent to AAK47354 from Mycobacterium tuberculosis strain CDC1551 (199 aa) but longer 42 aa. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6X5U4 No conserved hypotheticals Q50461 I6X5U4 I6X5U4 NC_000962.3 Mycobrowser_v5 CDS 3307580 3308545 . - 0 Rv2955c Rv2955c Function unknown Conserved protein Rv2955c, (MTCY349.34), len: 321 aa. Conserved protein, similar to others e.g. Q98NV5|MLL9724 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (284 aa), FASTA scores: opt: 231, E(): 6.5e-08, (34.6% identity in 182 aa overlap); Q9AGG2|NLPE1 NLPE1 from Rhizobium etli (249 aa), FASTA scores: opt: 212, E(): 1.1e-06, (27.85% identity in 255 aa overlap); Q9KXY2 hypothetical 31.3 KDA protein from Streptomyces coelicolor(291 aa), FASTA scores: opt: 211, E(): 1.4e-06, (30.9% identity in 249 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P95137 No conserved hypotheticals P95137 P95137 NC_000962.3 Mycobrowser_v5 CDS 3365836 3366450 . - 0 Rv3007c Rv3007c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv3007c, (MTV012.21c), len: 204 aa. Possible oxidoreductase, similar to Q9EWU5|3SC5B7.04c putative oxidoreductase from Streptomyces coelicolor (162 aa), FASTA scores: opt: 376, E(): 1.5e-18, (41.35% identity in 150 aa overlap); Q9K416|SCG22.29c putative flavin-dependent reductase protein from Streptomyces coelicolor (169 aa), FASTA scores: opt: 246, E(): 1e-09, (34.1% identity in 135 aa overlap); and some similarity to coupling proteins of 4-hydroxyphenylacetic hydroxylase/monooxygenase e.g. Q9HWT6|HPAC|PA4092 Pseudomonas aeruginosa (170 aa), FASTA score: opt: 214; O68232|HPAC Photorhabdus luminescens (Xenorhabdus luminescens) (172 aa), FASTA score: opt: 198; Q9RPU2|HPAC Salmonella dublin (170 aa), FASTA score: opt: 197; etc. Equivalent to AAK47416 from Mycobacterium tuberculosis strain CDC1551 (236 aa) but shorter 32 aa. Start chosen by similarity. O53254 No intermediary metabolism and respiration O53254 O53254 GO:0055114,GO:0016491,GO:0010181 1.-.-.- O53254 NC_000962.3 Mycobrowser_v5 CDS 3308668 3309399 . + 0 Rv2956 Rv2956 Function unknown Conserved protein Rv2956, (MTCY349.33c), len: 243 aa. Conserved protein, highly similar to O86299|GSC GSC protein from Mycobacterium avium subsp. silvaticum Mycobacterium avium (240 aa), FASTA scores: opt: 1070, E(): 3.5e-63, (67.5% identity in 240 aa overlap); and O86294|GSC GSC protein from Mycobacterium paratuberculosis (240 aa), FASTA scores: opt: 1070, E(): 3.5e-63, (67.5% identity in 240 aa overlap). Also some similarity with other proteins from other organisms e.g. Q9L727 nodulation protein NOEI from Rhizobium fredii (Sinorhizobium fredii) (241 aa), FASTA scores: opt: 205, E(): 3.5e-06, (27.25% identity in 198 aa overlap); Q9AGG1|LPEA LPEA protein from Rhizobium etli (286 aa), FASTA scores: opt: 201, E(): 7.2e-06, (28.85% identity in 208 aa overlap); P74191|SLL1173 hypothetical 28.0 KDA protein Synechocystis sp. (strain PCC 6803) (244 aa), FASTA scores: opt: 274, E(): 1e-10, (30.65% identity in 225 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. P71792|RV1513|MTCY277.35 hypothetical 26.7 KDA protein (243 aa), FASTA scores: opt: 1105, E(): 1.7e-65, (70.05% identity in 237 aa overlap); etc. Predicted to be an outer membrane protein (See Song et al., 2008). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6Y242 No conserved hypotheticals I6Y242 I6Y242 NC_000962.3 Mycobrowser_v5 CDS 3309470 3310297 . + 0 Rv2957 Rv2957 Function unknown; probably involved in cellular metabolism. Possible glycosyl transferase Rv2957, (MTCY349.31c), len: 275 aa. Possible glycosyl transferase ; possibly secreted protein. Highly similar to O88109|GSD|GTFD GSD protein from Mycobacterium avium subsp. silvaticum, Mycobacterium paratuberculosis, and Mycobacterium avium (266 aa), FASTA scores: opt: 1010, E(): 2.5e-62, (68.8% identity in 221 aa overlap). Also some similarity with other proteins and especially glycosyl transferases e.g. Q9AEE4 hypothetical 31.4 KDA protein from Leptospira interrogans (265 aa), FASTA scores: opt: 371, E(): 3.3e-18, (34.43% identity in 212 aa overlap); Q9EXY4 putative glycosyl transferase from Escherichia coli (248 aa), FASTA scores: opt: 339, E(): 5e-16, (32.4% identity in 210 aa overlap); Q9RCC4 glycosyltransferase-like protein from Yersinia pestis (247 aa), FASTA scores: opt: 333, E(): 1.3e-15, (31.8% identity in 217 aa overlap); Q9EXY1 putative glycosyl transferase from Escherichia coli (248 aa), FASTA scores: opt: 328, E(): 2.9e-15, (31.9% identity in 210 aa overlap); etc. Equivalent to AAK47357 from Mycobacterium tuberculosis strain CDC1551 (256 aa) but longer 19 aa. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WMX7 No intermediary metabolism and respiration P0A599 P9WMX7 GO:0016757 2.4.1.- P9WMX7 NC_000962.3 Mycobrowser_v5 CDS 3333785 3335164 . - 0 Rv2978c Rv2978c Required for the transposition of the insertion element IS1538. Probable transposase Rv2978c, (MTCY349.09), len: 459 aa. Probable transposase for IS1538, very similar to several other putative transposases from Mycobacterium tuberculosis e.g. YX16_MYCTU|Q10809 (460 aa), FASTA scores: opt: 2613, E(): 0, (83.0% identity in 458 aa overlap); etc. Low level matches to other tranposases. I6Y263 No insertion seqs and phages P95117 I6Y263 I6Y263 NC_000962.3 Mycobrowser_v5 CDS 3312101 3312838 . - 0 Rv2959c Rv2959c Thought to cause methylation. Possible methyltransferase (methylase) Rv2959c, (MTCY349.29), len: 245 aa. Possible methyltransferase, highly similar to Q9CD89|ML0127 from Mycobacterium leprae (229 aa), FASTA scores: opt: 1183, E(): 3.9e-69, (76.1% identity in 226 aa overlap). Also some similarity with other methyltransferases and other proteins e.g. Q51079 putative methyl transferase from Nocardia lactamdurans (236 aa), FASTA scores: opt: 156, E(): 0.0086, (23.25% identity in 159 aa overlap); Q98ID5 cephalosporin hydroxylase from Rhizobium loti (Mesorhizobium loti) (217 aa), FASTA scores: opt: 275, E(): 1.7e-10, (29.65% identity in 199 aa overlap); etc. And also similar to P72897 hypothetical 27.8 KDA protein from Mycobacterium tuberculosis (249 aa), FASTA scores: opt: 292, E(): 1.5e-11, (31.25% identity in 208 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WIM5 No intermediary metabolism and respiration Q50457 P9WIM5 GO:0008168,GO:0008610 2.1.1.- P9WIM5 NC_000962.3 Mycobrowser_v5 CDS 3313283 3313672 . + 0 Rv2961 Rv2961 Required for the transposition of an insertion element. Probable transposase Rv2961, (MTCY349.26c), len: 129 aa. Probable transposase, highly similar to C-terminus of O50414|Rv3387|MTV004.45 putative transposase from Mycobacterium tuberculosis (225 aa), FASTA scores: opt: 605, E(): 7.2e-34, (66.65% identity in 129 aa overlap); and similar to others e.g. CAC47401 putative partial transposase for ISRM17 protein from Rhizobium meliloti (Sinorhizobium meliloti) (174 aa), FASTA scores: opt: 183, E(): 2.6e-05, (30.25% identity in 129 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P95131 No insertion seqs and phages P95131 P95131 GO:0004803,GO:0006313,GO:0003677 NC_000962.3 Mycobrowser_v5 CDS 3367264 3368793 . - 0 Rv3009c gatB Component of the translational apparatus. Furnishes a means for formation of correctly charged GLN-tRNA(GLN) through the transamidation of misacylated GLU- tRNA(GLN) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-GLU-tRNA(GLN) [catalytic activity: ATP + L-glutamyl-tRNA(GLN) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(GLN) + L-glutamate]. Probable glutamyl-tRNA(GLN) amidotransferase (subunit B) GatB (Glu-ADT subunit B) Rv3009c, (MT3089, MTV012.23c), len: 509 aa. Probable gatB, Glu- tRNA-Gln amidotransferase, subunit B , equivalent to O33107|GATB_MYCLE|MLCB637_15 glutamyl-tRNA(GLN) amidotransferase from Mycobacterium leprae (509 aa), FASTA scores: opt: 2973, E(): 2.9e-173, (88.4% identity in 509 aa overlap). Also highly similar to other Glu- tRNA-Gln amidotransferases e.g. Q9Z578|GATB|SC8D9.13 from Streptomyces coelicolor (504 aa), FASTA scores: opt: 2264, E(): 3.6e-130, (66.0% identity in 495 aa overlap); P74215|GATB_SYNY3|SLL1435 from Synechocystis sp. strain PCC 6803 (519 aa), FASTA scores: opt: 1289, E(): 6.7e-71, (42.0% identity in 485 aa overlap); Q9X100|GATB_THEMA|TM1273 glutamyl-tRNA(GLN) amidotransferase from Thermotoga maritima (482 aa), FASTA scores: opt: 1165, E(): 2.2e-63, (40.05% identity in 487 aa overlap); etc. For more information about function, see citation below. Similar to many members of the pet112 family. Belongs to the GatB family. P9WN61 No information pathways P64199 P9WN61 GO:0050567,GO:0006412,GO:0005524 6.3.5.- P9WN61 NC_000962.3 Mycobrowser_v5 CDS 3318330 3318815 . - 0 Rv2965c kdtB Involved in the coenzyme A (CoA) biosynthesis (at the fourth step). Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (DPCOA) and pyrophosphate [catalytic activity: ATP + pantetheine 4'-phosphate = diphosphate + dephospho-CoA]. Probable phosphopantetheine adenylyltransferase KdtB (pantetheine- phosphate adenylyltransferase) (PPAT) (dephospho-CoA pyrophosphorylase) Rv2965c, (MTCY349.22), len: 161 aa. Probable kdtB (alternate gene name: coaD), phosphopantetheine adenylyltransferase, equivalent to O69466|COAD_MYCLE phosphopantetheine adenylyltransferase from Mycobacterium leprae (160 aa), FASTA scores: opt: 881, E(): 2.5e-54, (84.1% identity in 157 aa overlap). Also highly similar to others e.g. Q9ZBR1|COAD_STRCO from Streptomyces coelicolor (159 aa), FASTA scores: opt: 575, E(): 5.8e-33, (54.1% identity in 159 aa overlap); Q9WZK0|COAD_THEMA from Thermotoga maritima (161 aa), FASTA scores: opt: 509, E(): 2.4e-28, (50.0% identity in 154 aa overlap); P23875|COAD_ECOLICOAD|KDTB|B3634|Z5058|ECS4509 from Escherichia coli strain O157:H7 and K12 (159 aa), FASTA scores: opt: 459, E(): 7.3e-25, (45.15% identity in 155 aa overlap); etc. Belongs to the CoaD family. P9WPA5 No cell wall and cell processes 1TFU,3PNB,3LCJ,3NBA,3NBK P0A530 P9WPA5 GO:0015937,GO:0005737,GO:0004595,GO:0005524 2.7.7.3 P9WPA5 NC_000962.3 Mycobrowser_v5 CDS 4132518 4133519 . + 0 Rv3691 Rv3691 Function unknown Conserved protein Rv3691, (MTV025.039), len: 333 aa. Conserved protein, similar to Q9KYW4|SCE33.19 putative secreted protein from Streptomyces coelicolor (387 aa) FASTA scores: opt: 481, E(): 6e-23, (36.6% identity in 358 aa overlap). Equivalent to AAK48160 from Mycobacterium tuberculosis strain CDC1551 (381 aa) but shorter 48 aa. O69659 No conserved hypotheticals O69659 O69659 NC_000962.3 Mycobrowser_v5 CDS 3324573 3325703 . - 0 Rv2970c lipN Function unknown; lipolytic enzyme involved in cellular metabolism. Probable lipase/esterase LipN Rv2970c, (MTCY349.17), len: 376 aa. Probable lipN, lipase/esterase, similar to others e.g. Q9AA37|CC0771 putative esterase from Caulobacter crescentus (380 aa), FASTA scores: opt: 822, E(): 8e-46, (42.15% identity in 318 aa overlap); Q9XDR4 esterase HDE from petroleum-degrading bacterium HD-1 (317 aa), FASTA scores: opt: 738, E(): 2e-40, (48.85% identity in 262 aa overlap); O52270 lipase from Pseudomonas sp. (strain B11-1) (308 aa), FASTA scores: opt: 683, E(): 7.3e-37, (41.3% identity in 288 aa overlap); etc. Also similar to P71668 hypothetical 34.1 KDA protein from Mycobacterium tuberculosis (320 aa), FASTA scores: opt: 715, E(): 6.3e-39, (42.3% identity in 298 aa overlap). Equivalent to AAK47374 from Mycobacterium tuberculosis strain CDC1551 (309 aa) but longer 67 aa. P95125 No intermediary metabolism and respiration P95125 P95125 GO:0016787 3.1.1.- P95125 NC_000962.3 Mycobrowser_v5 CDS 3325934 3326104 . + 0 Rv2970A Rv2970A Function unknown Conserved hypothetical protein Rv2970A, len: 56 aa. Conserved hypothetical protein, similar to C-terminal part of several oxidoreductases e.g. Rv2971|Z83018|MTCY349_22 from Mycobacterium tuberculosis (282 aa), FASTA scores: opt: 158, E(): 3.6e-06, (45.0% identity in 60 aa overlap). May represent a gene fragment. I6XFT2 No conserved hypotheticals I6XFT2 I6XFT2 NC_000962.3 Mycobrowser_v5 CDS 3335164 3335748 . - 0 Rv2979c Rv2979c Prevents the cointegration of foreign DNA before integration into the chromosome. Probable resolvase Rv2979c, (MTCY349.08), len: 194 aa. Probable resolvase for IS1538, with low level matches to transposon resolvases; highly similar from aa 101 to YX1C_MYCTU|Q10831 from Mycobacterium tuberculosis (295 aa), FASTA scores: opt: 809, E(): 0, (69.1% identity in 194 aa overlap). Contains PS00397 Site-specific recombinases active site, and possible helix-turn-helix motiv at aa 2-23. I6XFU1 No insertion seqs and phages P95116 I6XFU1 GO:0006310,GO:0000150,GO:0003677 I6XFU1 NC_000962.3 Mycobrowser_v5 CDS 3363348 3363686 . + 0 Rv3004 cfp6 Unknown function (putative secreted protein). Low molecular weight protein antigen 6 (CFP-6) Rv3004, (MT3084.1, MTV012.18), len: 112 aa. Cfp6, low molecular weight protein antigen 6 (CFP-6) (See Bhaskar et al., 2000). Weak homology with Q9RKZ5|SC6D7.02 putative membrane protein from Streptomyces coelicolor (156 aa), FASTA scores: opt: 109, E(): 0.78, (39.4% identity in 122 aa overlap). Caution: the initiator methionine may be further upstream making the sequence a precursor. Predicted to be an outer membrane protein (See Song et al., 2008). P9WIR1 No cell wall and cell processes P0A5P2 P9WIR1 GO:0005576 NC_000962.3 Mycobrowser_v5 CDS 3327733 3329946 . - 0 Rv2973c recG Critical role in recombination and DNA repair. Help process holliday junction intermediates to mature products by catalysing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RECG unwind branched duplex DNA (Y-DNA). Probable ATP-dependent DNA helicase RecG Rv2973c, (MTCY349.14), len: 737 aa. Probable recG, ATP-dependent DNA helicase (see citation below), equivalent to O69460|RECG_MYCLE ATP-dependent DNA helicase from Mycobacterium leprae (743 aa), FASTA scores: opt: 3846, E(): 0, (79.3% identity in 744 aa overlap). Also highly similar to others e.g. Q9ZBR3|SC7A1.10 putative ATP-dependent DNA helicase from Streptomyces coelicolor (742 aa), FASTA scores: opt: 1249, E(): 1.1e-67, (46.2% identity in 758 aa overlap); Q9PGE8 ATP-dependent DNA helicase from Xylella fastidiosa (718 aa), FASTA scores: opt: 1174, E(): 3.5e-63, (42.1% identity in 539 aa overlap); P24230|RECG_ECOLI|RECG|B3652 from Escherichia coli strain K12 (693 aa), FASTA scores: opt: 457, E(): 7.3e-22, (35.2% identity in 733 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the helicase family, RECG subfamily. P9WMQ7 No information pathways P64322 P9WMQ7 GO:0004003,GO:0003677,GO:0006310,GO:0006281,GO:0005524 3.6.4.12 P9WMQ7 NC_000962.3 Mycobrowser_v5 CDS 3329949 3331361 . - 0 Rv2974c Rv2974c Function unknown Conserved hypothetical alanine rich protein Rv2974c, (MTCY349.13), len: 470 aa. Conserved hypothetical ala-rich protein, highly similar to others e.g. C-terminus of Q9ZBR4|SC7A1.09 hypothetical 59.5 KDA protein from Streptomyces coelicolor (589 aa), FASTA scores: opt: 774, E(): 1.3e-36, (41.0% identity in 495 aa overlap); Q9K9Z6|BH2498 hypothetical protein from Bacillus halodurans (557 aa), FASTA scores: opt: 268, E(): 8e-08, (27.7% identity in 502 aa overlap) (N-terminus longer 76 aa); Q9X293 conserved hypothetical protein from Thermotoga maritima (497 aa), FASTA scores: opt: 265, E(): 1.1e-07, (24.9% identity in 470 aa overlap) (N-terminus longer 43 aa); etc. Also some similarity with P47609|Y369_MYCGE|MG369 hypothetical protein from Mycoplasma genitalium (557 aa), FASTA scores: opt: 154, E(): 0.25, (20.25% identity in 489 aa overlap); this, and following ORF, are similar to Y369_MYCGE but no cosmid sequence error was identified. I6Y259 No conserved hypotheticals P95121 I6Y259 GO:0004371,GO:0006071 I6Y259 NC_000962.3 Mycobrowser_v5 CDS 3331358 3331612 . - 0 Rv2975c Rv2975c Function unknown Conserved hypothetical protein Rv2975c, (MTCY349.12), len: 84 aa. Conserved hypothetical protein, similar to N-terminus of others e.g. Q9ZBR4|SC7A1.09 hypothetical 59.5 KDA protein from Streptomyces coelicolor (589 aa), FASTA scores: opt: 141, E(): 0.0019, (41.25% identity in 80 aa overlap); Q98R49|MYPU_1610 hypothetical protein from Mycoplasma pulmonis (545 aa), FASTA scores: opt: 127, E(): 0.023, (48.0% identity in 50 aa overlap); Q9K9Z6|BH2498 hypothetical protein from Bacillus halodurans (557 aa), FASTA scores: opt: 126, E(): 0.028, (34.55% identity in 81 aa overlap); etc. Also some similarity with N-terminus of P47609|Y369_MYCGE|MG369 hypothetical protein from Mycoplasma genitalium (557 aa), FASTA scores: opt: 108, E(): 0.7, (36.75% identity in 49 aa overlap); this, and preceding ORF, are similar to Y369_MYCGE and YLOV protein but no cosmid sequence error was identified. P95120 No conserved hypotheticals P95120 GO:0004371,GO:0006071 P95120 NC_000962.3 Mycobrowser_v5 CDS 3488089 3488559 . + 0 Rv3122 Rv3122 Unknown Hypothetical protein Rv3122, (MTCY164.32), len: 156 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07033 No conserved hypotheticals O07033 NC_000962.3 Mycobrowser_v5 CDS 3335960 3336505 . + 0 Rv2980 Rv2980 Unknown Possible conserved secreted protein Rv2980, (MTCY349.07c), len: 181 aa. Possible conserved secreted protein, equivalent to Q9CBS1 possible secreted protein from Mycobacterium leprae (191 aa), FASTA scores: opt: 794, E(): 2.3e-40, (67.25% identity in 177 aa overlap). Also some weak similarity with other hypothetical proteins or secreted proteins e.g. C-terminus of Q98F98|MLL3872 MLL3872 protein from Rhizobium loti (Mesorhizobium loti) (575 aa), FASTA scores: opt: 148, E(): 0.16, (28.35% identity in 194 aa overlap); Q9L0W9|SCH22A.13C putative secreted protein from Streptomyces coelicolor (167 aa), FASTA scores: opt: 114, E(): 7.5, (40.0% identity in 80 aa overlap); etc. Equivalent to AAK47385 from Mycobacterium tuberculosis strain CDC1551 (214 aa) but shorter 33 aa. Has hydrophobic stretch near N-terminus. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). P95115 No cell wall and cell processes P95115 P95115 NC_000962.3 Mycobrowser_v5 CDS 3481451 3482698 . + 0 Rv3115 Rv3115 Involved in the transposition in the insertion sequence IS1081. Probable transposase Rv3115, (MTCY164.25), len: 415 aa. Probable IS1081 transposase, similar to others. Has transposases, mutator family, signature (PS01007). Other copies are MTCY10G2.02c, MTCY441.35, MTCY77.03c. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P96354 No insertion seqs and phages P96354 P96354 GO:0004803,GO:0006313,GO:0003677 P96354 NC_000962.3 Mycobrowser_v5 CDS 3550715 3551044 . + 0 Rv3183 Rv3183 Involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv3183, (MTV014.27), len: 109 aa. Possible transcriptional regulator, similar to others e.g. Q9S1D9|YPPCP1.08c from Yersinia pestis (99 aa), FASTA scores: opt: 119, E(): 0.47, (40.55% identity in 74 aa overlap); Q9X153|TM1330 from Thermotoga maritima (111 aa), FASTA scores: opt: 115, E(): 0.91, (40.35% identity in 57 aa overlap); P95258|Rv1956|MTCY09F9.08c (alias AAK46277 putative DNA-binding protein from strain CDC1551) (149 aa), FASTA scores: opt: 116, E(): 1, (42.25% identity in 71 aa overlap). Also similar to O53467|Rv2021c|MTV018.08c from Mycobacterium tuberculosis (101 aa), FASTA scores: opt: 214, E(): 5.8e-07, (43.0% identity in 107 aa overlap). Contains probable helix-turn-helix motif from aa 51 to 72 (Score 1803, +5.33 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53333 No regulatory proteins O53333 O53333 GO:0043565 O53333 NC_000962.3 Mycobrowser_v5 CDS 3337995 3338999 . - 0 Rv2982c gpdA2 Involved in de novo phospholipid biosynthesis; glycerol-3 phosphate formation [catalytic activity: SN-glycerol 3-phosphate + NAD(P)(+) = glycerone phosphate + NAD(P)H]. Probable glycerol-3-phosphate dehydrogenase [NAD(P)+] GpdA2 (NAD(P)H- dependent glycerol-3-phosphate dehydrogenase) Rv2982c, (MTCY349.05), len: 334 aa. Probable gpdA2 (alternate gene name: gpsA), glycerol-3-phosphate dehydrogenase [NAD(P)+], equivalent to Q9CBR9|GPDA_MYCLE glycerol-3-phosphate dehydrogenase [NAD(P)+] from Mycobacterium leprae (349 aa), FASTA scores: opt: 1686, E(): 1.7e-95, (77.95% identity in 349 aa overlap). Also highly similar to others e.g. Q9ZBS0|GPDA_STRCO from Streptomyces coelicolor (336 aa), FASTA scores: opt: 1165, E(): 9.8e-64, (56.25% identity in 327 aa overlap); P46919|GPDA_BACSU from Bacillus subtilis (345 aa), FASTA scores: opt: 872, E(): 7.5e-46, (44.9% identity in 325 aa overlap); P37606|GPDA_ECOLI|GPSA|B3608|Z5035|ECS4486. from Escherichia coli strain O157:H7 and K12 (339 aa), FASTA scores: opt: 799, E(): 2.1e-41, (42.9% identity in 331 aa overlap); etc. Also highly similar to O53761|GPD2_MYCTU probable glycerol-3-phosphate dehydrogenase from Mycobacterium tuberculosis (341 aa), FASTA scores: opt: 740, E(): 8.4e-38, (40.35% identity in 322 aa overlap). Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family. P9WN77 No lipid metabolism P95113 P9WN77 GO:0046167,GO:0046168,GO:0004367,GO:0047952,GO:0009331,GO:0055114,GO:0008654,GO:0051287 1.1.1.94 P9WN77 NC_000962.3 Mycobrowser_v5 CDS 3339118 3339762 . + 0 Rv2983 Rv2983 Function unknown Conserved hypothetical alanine rich protein Rv2983, (MTCY349.04c), len: 214 aa. Conserved hypothetical ala-rich protein, equivalent to O33128|ML1680|MLCB637.37c hypothetical 22.0 KDA protein from Mycobacterium leprae (216 aa), FASTA scores: opt: 1080, E(): 9e-61, (79.05% identity in 215 aa overlap). Also similar to other hypothetical proteins e.g. Q9ZBS2|SC7A1.01C from Streptomyces coelicolor (212 aa), FASTA scores: opt: 420, E(): 2.9e-19, (43.5% identity in 207 aa overlap); O26710|MTH613 from Methanothermobacter thermautotrophicus (223 aa), FASTA scores: opt: 193, E(): 5.8e-05, (30.0% identity in 190 aa overlap); Q9RKG8|SCE46.21 from Streptomyces coelicolor (210 aa), FASTA scores: opt: 139, E(): 0.14, (27.65% identity in 206 aa overlap); etc. P9WP83 No conserved hypotheticals P95112 P9WP83 P9WP83 NC_000962.3 Mycobrowser_v5 CDS 3347982 3348473 . + 0 Rv2991 Rv2991 Function unknown Conserved protein Rv2991, (MTV012.05), len: 163 aa. Conserved protein, similar to others e.g. Q9K3X7|2SCG61.39. hypothetical 17.6 KDA protein from Streptomyces coelicolor (153 aa), FASTA scores: opt: 266, E(): 2.1e-11, (34.85% identity in 155 aa overlap); Q9CNX3|PM0299 hypothetical protein from Pasteurella multocida (171 aa), FASTA scores: opt: 175, E(): 5.1e-05, (31.3% identity in 131 aa overlap); Q9KZI9|SCG8A.10 conserved hypothetical protein from Streptomyces coelicolor (142 aa), FASTA scores: opt: 163, E(): 0.00031, (32.4% identity in 108 aa overlap); etc. Also some similarity to O06553|MTCI65.22|Rv1155 hypothetical protein from Mycobacterium tuberculosis (147 aa), FASTA scores: opt: 127, E(): 0.1, (32.9% identity in 73 aa overlap); and to several proteins of similar size that confer resistance to 5-Nitroimidazole antibiotics in Bacteroides. O53240 No conserved hypotheticals 1RFE O53240 O53240 GO:0010181 O53240 NC_000962.3 Mycobrowser_v5 CDS 3348805 3350277 . - 0 Rv2992c gltS Involved in translation mechanisms [catalytic activity: ATP + L-glutamate + tRNA(GLU) = AMP + diphosphate + L-glutamyl-tRNA(GLU)]. Glutamyl-tRNA synthetase GltS (glutamate--tRNA ligase) (glutamyl-tRNA synthase) (GLURS) Rv2992c, (MTV012.06c), len: 490 aa. GltS (alternate gene name: gltX), glutamyl-tRNA synthase, equivalent to O33120|SYE_MYCLE glutamyl-tRNA synthetase from Mycobacterium leprae (502 aa), FASTA scores: opt: 2660, E(): 2.3e-163, (81.35% identity in 488 aa overlap). Also highly similar to others e.g. O86528|SYE_STRCO from Streptomyces coelicolor (494 aa), FASTA scores: opt: 1777, E(): 1.4e-106, (57.45% identity in 484 aa overlap); P22250|SYE_BACSU from Bacillus subtilis (483 aa), FASTA scores: opt: 1099, E(): 5.4e-63, (38.45% identity in 489 aa overlap); O51345|SYE_BORBU|GLTX|BB0372 from Borrelia burgdorferi (Lyme disease spirochete) (490 aa), FASTA scores: opt: 1009, E(): 3.3e-57, (34.85% identity in 491 aa overlap); etc. Belongs to class-I aminoacyl-tRNA synthetase family. P9WFV9 No information pathways 2JA2,3PNV,3PNY P0A636 P9WFV9 GO:0005737,GO:0004818,GO:0006424,GO:0005524 6.1.1.17 P9WFV9 NC_000962.3 Mycobrowser_v5 CDS 3343176 3343820 . - 0 Rv2986c hupB This protein belongs to the histone like family of prokaryotic DNA-binding proteins which are capable of wrapping DNA to stabilize it, and prevent its denaturation under extreme environmental conditions. DNA-binding protein HU homolog HupB (histone-like protein) (HLP) (21-kDa laminin-2-binding protein) Rv2986c, (MTCY349.01), len: 214 aa. hupB (alternate gene names: hup, hlp, lbp21), DNA-binding protein HU homolog (resembles fusion between HU and histone) (see Pethe et al., 2002), equivalent to others from Mycobacteria e.g. Q9XB18|DBH_MYCBO from Mycobacterium bovis (205 aa), FASTA scores: opt: 1050, E(): 5.6e-45, (95.35% identity in 214 aa overlap); Q9ZHC5|DBH_MYCSM from Mycobacterium smegmatis (208 aa), FASTA scores: opt: 1035, E(): 3.1e-44, (80.2% identity in 217 aa overlap); and O33125|DBH_MYCLE from Mycobacterium leprae (200 aa), FASTA scores: opt: 914, E(): 2.7e-38, (80.1% identity in 216 aa overlap). Also highly similar to others from other organisms e.g. O86537|DBH2_STRCO from Streptomyces coelicolor (218 aa), FASTA scores: opt: 569, E(): 2.6e-21, (51.35% identity in 220 aa overlap); P08821|DBH1_BACSU from Bacillus subtilis (92 aa), FASTA scores: opt: 280, E(): 2.5e-07, (45.05% identity in 91 aa overlap) (C-terminus shorter); etc. Contains PS00045 Bacterial histone-like DNA-binding proteins signature. Belongs to the bacterial histone-like protein family. Note that its C-terminal domain is very rich in lysine and alanine. P9WMK7 No information pathways 3C4I P95109 P9WMK7 GO:0030261,GO:0003677 P9WMK7 NC_000962.3 Mycobrowser_v5 CDS 3344033 3344629 . - 0 Rv2987c leuD Involved in leucine biosynthesis (at the second step) [catalytic activity: 3-isopropylmalate = 2-isopropylmaleate + H(2)O (also catalyses 2-isopropylmaleate + H(2)O = 3-hydroxy-4-methyl-3-carboxypentanone)]. Probable 3-isopropylmalate dehydratase (small subunit) LeuD (isopropylmalate isomerase) (alpha-IPM isomerase) (IPMI) Rv2987c, (MTV012.01c), len: 198 aa. Probable leuD, 3-isopropylmalate dehydratase, small subunit, equivalent to O33124|LEUD_MYCLE 3-isopropylmalate dehydratase small subunit from Mycobacterium leprae (198 aa), FASTA scores: opt: 1155, E(): 4.2e-72, (87.75% identity in 196 aa overlap). Also highly similar to many e.g. O86535|LEUD_STRCO from Streptomyces coelicolor (197 aa), FASTA scores: opt: 765, E(): 2.6e-45, (59.0% identity in 195 aa overlap); P04787|LEUD_SALTY from Salmonella typhimurium (201 aa), FASTA scores: opt: 528, E(): 5.2e-29, (45.05% identity in 191 aa overlap); P30126|LEUD_ECOLI|LEUD|B0071 from Escherichia coli strain K12 (201 aa), FASTA scores: opt: 498, E(): 6e-27, (43.45% identity in 191 aa overlap); etc. P9WK95 No intermediary metabolism and respiration 3H5E,3H5H,3H5J P65277 P9WK95 GO:0009316,GO:0009098,GO:0003861 4.2.1.33 P9WK95 NC_000962.3 Mycobrowser_v5 CDS 3344654 3346075 . - 0 Rv2988c leuC Involved in leucine biosynthesis (at the second step) [catalytic activity: 3-isopropylmalate = 2-isopropylmaleate + H(2)O (also catalyses 2-isopropylmaleate + H(2)O = 3-hydroxy-4-methyl-3-carboxypentanone)]. Probable 3-isopropylmalate dehydratase (large subunit) LeuC (isopropylmalate isomerase) (alpha-IPM isomerase) (IPMI) Rv2988c, (MTV012.02c), len: 473 aa. Probable leuC, 3-isopropylmalate dehydratase, large subunit, equivalent to O33123|LEU2_MYCLE 3-isopropylmalate dehydratase small subunit from Mycobacterium leprae (476 aa), FASTA scores: opt: 2818, E(): 1.3e-171, (88.75% identity in 471 aa overlap). Also highly similar to many e.g. Q44427|LEU2_ACTTI from Actinoplanes teichomyceticus (485 aa), FASTA scores: opt: 1958, E(): 6.5e-117, (71.0% identity in 479 aa overlap); P55251|LEU2_RHIPU from Rhizomucor pusillus (755 aa), FASTA scores: opt: 1937, E(): 1.9e-115, (61.25% identity in 467 aa overlap) (C-terminus longer); P30127|LEU2_ECOLI|LEUC|B0072 from Escherichia coli strain K12 (465 aa), FASTA scores: opt: 1896, E(): 5.5e-113, (61.6% identity in 456 aa overlap); etc. Contains PS00450 Aconitase family signature. Belongs to the aconitase/IPM isomerase family. P9WQF5 No intermediary metabolism and respiration O53237 P9WQF5 GO:0051539,GO:0009098,GO:0046872,GO:0003861 4.2.1.33 P9WQF5 NC_000962.3 Mycobrowser_v5 CDS 3842239 3842769 . + 0 Rv3425 PPE57 Function unknown PPE family protein PPE57 Rv3425, (MTCY78.04c), len: 176 aa. PPE57, Member of the M. tuberculosis PPE family, similar to many e.g. O06246|Rv3429|MTCY77.01 (178 aa), FASTA scores: opt: 781, E(): 7e-44, (69.9% identity in 176 aa overlap); and downstream Q50702|YY26_MYCTU|Rv3426|MTCY78.03c (232 aa), FASTA scores: opt: 517, E(): 1.2e-26, (68.0% identity in 125 aa overlap); MTV049_11, MTCY428_16, MTV049_22, MTV049_30, MTCY261_4; etc. Q50703 No PE/PPE Q50703 Q50703 Q50703 NC_000962.3 Mycobrowser_v5 CDS 3346859 3347719 . - 0 Rv2990c Rv2990c Unknown Hypothetical protein Rv2990c, (MTV012.04c), len: 286 aa. Hypothetical unknown protein. I6YEW1 No conserved hypotheticals I6YEW1 I6YEW1 NC_000962.3 Mycobrowser_v5 CDS 3743711 3753184 . - 0 Rv3347c PPE55 Function unknown PPE family protein PPE55 Rv3347c, (MTV004.03c), len: 3157 aa. PPE55, Member of the Mycobacterium tuberculosis PPE family, Gly-, Ala-, Asn-rich protein. Similar to many from Mycobacterium tuberculosis strains H37Rv and CDC1551, e.g. O50379|Rv3350c|MTV004.07c (3716 aa), FASTA scores: opt: 6497, E(): 0, (61.65% identity in 3756 aa overlap); and other upstream ORFs MTV004_5, MTY13E10_15, MTCY28_16, MTCY63_9, MTY13E10_17, MTCY180_1; etc. Predicted possible vaccine candidate (See Zvi et al., 2008). Q6MWX9 No PE/PPE Q6MWX9 Q6MWX9 Q6MWX9 NC_000962.3 Mycobrowser_v5 CDS 3357225 3357428 . - 0 Rv2998A Rv2998A Function unknown Conserved hypothetical protein Rv2998A, len: 67 aa. Probable conserved hypothetical protein, (possibly gene fragment), highly similar to central part of two-component sensor proteins e.g. O07777|Rv0601c|MTCY19H5.21 two component sensor (fragment) from Mycobacterium tuberculosis (156 aa), FASTA scores: opt: 212, E(): 3.7e-09, (58.2% identity in 67 aa overlap); Q9L2B6|SC8F4.08 probable two-component sensor kinase from Streptomyces coelicolor (478 aa), FASTA scores: opt: 193, E(): 2.6e-07, (47.05% identity in 68 aa overlap); etc. Q6MX20 No conserved hypotheticals Q6MX20 GO:0004871,GO:0007165,GO:0016021 Q6MX20 NC_000962.3 Mycobrowser_v5 CDS 3353483 3355069 . - 0 Rv2996c serA1 Involved at the first committed step in the 'phosphorylated' pathway of L-serine biosynthesis [catalytic activity: 3-phosphoglycerate + NAD(+) = 3-phosphohydroxypyruvate + NADH]. Probable D-3-phosphoglycerate dehydrogenase SerA1 (PGDH) Rv2996c, (MTV012.10), len: 528 aa. Probable serA1, D-3-phosphoglycerate dehydrogenase, equivalent to SERA_MYCLE D-3-phosphoglycerate dehydrogenase from Mycobacterium leprae (528 aa), FASTA scores: opt: 2974, E(): 1.9e-166, (89.6% identity in 528 aa overlap). Also highly similar to many e.g. Q9Z564 from Streptomyces coelicolor (529 aa), FASTA scores: opt: 1879, E(): 2.1e-102, (57.6% identity in 526 aa overlap); O29445|SERA_ARCFU from Archaeoglobus fulgidus (527 aa), FASTA scores: opt: 1252, E(): 9.6e-66, (41.3% identity in 530 aa overlap); P35136|SERA_BACSU from Bacillus subtilis (525 aa), FASTA scores: opt: 1172, E(): 4.5e-61, (37.9% identity in 528 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00065 D-isomer specific 2-hydroxyacid dehydrogenases NAD-binding signature, and PS00670 D-isomer specific 2-hydroxyacid dehydrogenases signature 2. Belongs to the D-isomer specific 2-hydroxyacid dehydrogenases family. Note that previously known as serA. P9WNX3 No intermediary metabolism and respiration 1YGY,3DC2,3DDN P0A544 P9WNX3 GO:0051287,GO:0016597,GO:0055114,GO:0004617,GO:0006564 1.1.1.95 P9WNX3 NC_000962.3 Mycobrowser_v5 CDS 3355099 3356541 . + 0 Rv2997 Rv2997 Function unknown; probably involved in cellular metabolism. Possible alanine rich dehydrogenase Rv2997, (MTV012.11), len: 480 aa. Possible ala-rich dehydrogenase, similar to others dehydrogenases and hypothetical proteins e.g. Q9EYI5 putative dehydrogenase from Streptomyces nogalater (472 aa), FASTA scores: opt: 1131, E(): 1.7e-61, (41.0% identity in 471 aa overlap); Q9ZBG4|SC9B5.16 putative dehydrogenase from Streptomyces coelicolor (472 aa), FASTA scores: opt: 1064, E(): 2e-57, (39.05% identity in 471 aa overlap); Q98BS8 probable dehydrogenase from Rhizobium loti (Mesorhizobium loti) (524 aa), FASTA scores: opt: 196, E(): 0.00021, (25.1% identity in 526 aa overlap); etc. Shows strong similarity throughout its length to O06826|MTCY493.22c|Rv1432 hypothetical 50.5 KDA protein from Mycobacterium tuberculosis (473 aa), FASTA scores: opt: 1220, E(): 6.1e-67, (42.35% identity in 465 aa overlap). I6YAU3 No intermediary metabolism and respiration I6YAU3 GO:0055114,GO:0016491 1.-.-.- NC_000962.3 Mycobrowser_v5 CDS 3356815 3357276 . + 0 Rv2998 Rv2998 Unknown Hypothetical protein Rv2998, (MTV012.12), len: 153 aa. Hypothetical unknown protein. Note that equivalent to AAK47405 Hypothetical 19.4 kDa protein from Mycobacterium tuberculosis strain CDC1551 (186 aa) but sequence differs in N-terminus. O53245 No conserved hypotheticals O53245 NC_000962.3 Mycobrowser_v5 CDS 3371815 3372471 . + 0 Rv3013 Rv3013 Function unknown Conserved protein Rv3013, (MTV012.27), len: 218 aa. Conserved protein, equivalent to O33103|MLCB637_11c hypothetical 24.4 KDA protein from Mycobacterium leprae (230 aa), FASTA scores: opt: 1188, E(): 2.6e-67, (83.95% identity in 218 aa overlap). Equivalent to AAK47422 from Mycobacterium tuberculosis strain CDC1551 (240 aa) but shorter 22 aa. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53260 No conserved hypotheticals O53260 O53260 NC_000962.3 Mycobrowser_v5 CDS 3549254 3549688 . - 0 Rv3180c Rv3180c Unknown Hypothetical alanine rich protein Rv3180c, (MTV014.24c), len: 144 aa. Hypothetical unknown ala-rich protein. Contains probable coiled-coil domain from aa 40 to 70. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WF51 No conserved hypotheticals P9WF51 P9WF51 NC_000962.3 Mycobrowser_v5 CDS 3358612 3359271 . + 0 Rv3000 Rv3000 Unknown Possible conserved transmembrane protein Rv3000, (MTV012.14), len: 219 aa. Possible conserved transmembrane protein, similar to various membrane proteins e.g. P77307|YBBM_ECOLI|B0491 hypothetical 28.2 KDA protein (potential integral membrane protein) from Escherichia coli strain K12 (259 aa), FASTA scores: opt: 292, E(): 3.1e-11, (30.25% identity in 218 aa overlap); N-terminus of Q9BJF3 putative ABC transporter (fragment) from Sterkiella histriomuscorum (1319 aa), FASTA scores: opt: 274, E(): 1.3e-09, (39.6% identity in 101 aa overlap); Q9C9W0|T23K23.21 putative ABC transporter from Arabidopsis thaliana (Mouse-ear cress) (263 aa), FASTA scores: opt: 258, E(): 4.4e-09, (30.1% identity in 196 aa overlap); P74369|YG47_SYNY3|SLR1647 hypothetical 28.1 KDA protein (potential integral membrane protein) from Synechocystis sp. strain PCC 6803 (259 aa), FASTA scores: opt: 257, E(): 5.1e-09, (37.75% identity in 98 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). I6X5Z8 No cell wall and cell processes O53247 I6X5Z8 GO:0016887,GO:0016021,GO:0005524 I6X5Z8 NC_000962.3 Mycobrowser_v5 CDS 3369950 3371434 . - 0 Rv3011c gatA Component of the translational apparatus. Furnishes a means for formation of correctly charged GLN-tRNA(GLN) through the transamidation of misacylated GLU-tRNA(GLN) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-GLU-tRNA(GLN) [catalytic activity: ATP + L-glutamyl-tRNA(GLN) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(GLN) + L-glutamate]. Probable glutamyl-tRNA(GLN) amidotransferase (subunit A) GatA (Glu-ADT subunit A) Rv3011c, (MT3091, MTV012.25c), len: 494 aa. Probable gatA, Glu-tRNA-Gln amidotransferase, subunit A , equivalent to O33105|GATA|ML1702|MLCB637.13 glutamyl-tRNA(GLN) amidotransferase from Mycobacterium leprae (497 aa), FASTA scores: opt: 2839, E(): 3.5e-161, (88.8% identity in 492 aa overlap). Also highly similar to other Glu-tRNA-Gln amidotransferases e.g. Q9Z580|GATA_STRCO from Streptomyces coelicolor (497 aa), FASTA scores: opt: 2231, E(): 4.5e-125, (70.3% identity in 486 aa overlap); P73558|GATA_SYNY3|SLR0877 from Synechocystis sp. strain PCC 6803 (483 aa), FASTA scores: opt: 1593, E(): 3.3e-87, (55.85% identity in 487 aa overlap); O06491|GATA_BACSU glutamyl-tRNA(GLN) amidotransferase from Bacillus subtilis (485 aa), FASTA scores: opt: 1389, E(): 4.3e-75, (51.7% identity in 468 aa overlap); etc. For more information about function, see citation below. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the amidase family. Nucleotide position 3370177 in the genome sequence has been corrected, T:G resulting in M420L. P9WQA1 No information pathways O53258 P9WQA1 GO:0050567,GO:0006412,GO:0005524 6.3.5.- P9WQA1 NC_000962.3 Mycobrowser_v5 CDS 3359585 3360586 . - 0 Rv3001c ilvC Involved in valine and isoleucine biosynthesis (at the second step) [catalytic activity: (R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH]. Probable KETOL-acid reductoisomerase IlvC (acetohydroxy-acid isomeroreductase) (alpha-keto-beta-hydroxylacil reductoisomerase) Rv3001c, (MT3081, MTV012.15c), len: 333 aa. Probable ilvC, ketol-acid reductoisomerase, equivalent or highly similar to others e.g. Q59500|ILVC_MYCAV from Mycobacterium avium (333 aa), FASTA scores: opt: 1977, E(): 3.2e-113, (87.7% identity in 333 aa overlap); O33114|ILVC_MYCLE from Mycobacterium leprae (333 aa), FASTA scores: opt: 1924, E(): 5.3e-110, (86.5% identity in 333 aa overlap); Q9Z565|ILVC_STRCO|SC8D9.26 from Streptomyces coelicolor (332 aa), FASTA scores: opt: 1494, E(): 8.3e-84, (67.5% identity in 326 aa overlap); Q59818|ILVC_STRAW from Streptomyces avermitilis (333 aa) FASTA scores: opt: 1487, E(): 2.2e-83, (66.8% identity in 326 aa overlap); etc. Belongs to the KETOL-acid reductoisomerases family. P9WKJ7 No intermediary metabolism and respiration P65149 P9WKJ7 GO:0009097,GO:0004455,GO:0055114,GO:0009099 1.1.1.86 P9WKJ7 NC_000962.3 Mycobrowser_v5 CDS 3364709 3365830 . + 0 Rv3006 lppZ Unknown Probable conserved lipoprotein LppZ Rv3006, (MTV012.20), len: 373 aa. Probable lppZ, conserved lipoprotein, equivalent to O33109|MLCB637.17C|ML1699 putative lipoprotein from M. leprae (372 aa), FASTA scores: opt: 2211, E(): 4.3e-100, (87.1% identity in 373 aa overlap). Shows also similarity (in part) with Q9Z571|SC8D9.20c putative oxidoreductase from Streptomyces coelicolor (447 aa), FASTA scores: opt: 185, E(): 0.051, (31.6% identity in 300 aa overlap); Q9Z9R3|BH2090 glucose dehydrogenase-B from Bacillus halodurans (371 aa), FASTA scores: opt: 206, E(): 0.0043, (28.3% identity in 205 aa overlap); and other glucose dehydrogenases B. Contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site, followed by a proline-rich domain. I6Y293 No cell wall and cell processes I6Y293 GO:0003824 I6Y293 NC_000962.3 Mycobrowser_v5 CDS 3396458 3397141 . - 0 Rv3036c TB22.2 Unknown Probable conserved secreted protein TB22.2 Rv3036c, (MTV012.51c), len: 227 aa. Probable TB22.2, conserved secreted protein, with putative N-terminal signal peptide, highly similar to secreted immunogenic protein MPT64/MPB64 P19996|Rv1980c|MTCY39.39 from Mycobacterium tuberculosis and Mycobacterium bovis (228 aa), FASTA scores: opt: 681, E(): 2.5e-35, (45.8% identity in 227 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). I6YF08 No cell wall and cell processes O53283 I6YF08 NC_000962.3 Mycobrowser_v5 CDS 3509118 3509546 . - 0 Rv3142c Rv3142c Unknown Hypothetical protein Rv3142c, (MTCY03A2.16), len: 142 aa. Hypothetical unknown protein. Equivalent to AAK47569 from Mycobacterium tuberculosis strain CDC1551 but shorter 33 aa. P95184 No conserved hypotheticals P95184 NC_000962.3 Mycobrowser_v5 CDS 3774482 3774874 . - 0 Rv3364c Rv3364c Function unknown Conserved protein Rv3364c, (MTV004.21c), len: 130 aa. Conserved protein, highly similar to others from Streptomyces coelicolor e.g. O86524|SC1C2.24c (137 aa), FASTA scores: opt: 466, E(): 1.3e-22, (58.6% identity in 116 aa overlap); O86521|SC1C2.20c (140 aa), FASTA scores: opt: 445, E(): 2.7e-21, (56.9% identity in 116 aa overlap); Q9KZI6|SCG8A.13c (145 aa), FASTA scores: opt: 341, E(): 9.5e-15, (51.3% identity in 113 aa overlap); etc. O50393 No conserved hypotheticals O50393 O50393 O50393 NC_000962.3 Mycobrowser_v5 CDS 3363693 3364532 . - 0 Rv3005c Rv3005c Function unknown Conserved hypothetical protein Rv3005c, (MTV012.19c), len: 279 aa. Conserved hypothetical protein, equivalent to O33110|MLCB637.18|ML1698 hypothetical 29.5 KDA protein from Mycobacterium leprae (277 aa), FASTA scores: opt: 1245, E(): 1.2e-65, (70.5% identity in 278 aa overlap). Also similar, but longer approximately 100 aa in N-terminus, to other hypothetical proteins, few membrane proteins, e.g. Q9RKN9|SCC75A.35 putative membrane protein from Streptomyces coelicolor (180 aa), FASTA scores: opt: 326, E(): 3.9e-12, (44.2% identity in 138 aa overlap); P96694|YDFP|AB001488 hypothetical protein from Bacillus subtilis (129 aa), FASTA scores: opt:273, E(): 3.7e-09, (33.1% identity in 130 aa overlap); Q9KKT1|VCA1019 hypothetical protein from Vibrio cholerae (148 aa), FASTA scores: opt: 258, E(): 3.1e-08, (34.9% identity in 126 aa overlap); etc. I6YAV3 No conserved hypotheticals O53252 I6YAV3 NC_000962.3 Mycobrowser_v5 CDS 3368823 3369854 . - 0 Rv3010c pfkA Involved in glycolysis; converts sugar-1-P to sugar-1,6-P [catalytic activity: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate]. Probable 6-phosphofructokinase PfkA (phosphohexokinase) (phosphofructokinase) Rv3010c, (MTV012.24c), len: 343 aa. Probable pfkA, phosphofructokinase, equivalent to O33106|K6PF_MYCLE|MLCB637.14 6-phosphofructokinase from Mycobacterium leprae (343 aa), FASTA scores: opt: 2099, E(): 4.1e-122, (90.4% identity in 343 aa overlap). Also highly similar to others e.g. Q9FC99|K6P3_STRCO from Streptomyces coelicolor (341 aa), FASTA scores: opt: 1329, E(): 1.1e-74, (58.9% identity in 338 aa overlap); Q9L1L8|K6P2_STRCO|PFKA2|PFK2|SC6A11.02 6-phosphofructokinase 2 from Streptomyces coelicolor (341 aa), FASTA scores: opt: 1303, E(): 4.5e-73, (56.7% identity in 342 aa overlap); Q9KH71|PFP PPI-dependent phosphofructokinase from Dictyoglomus thermophilum (346 aa), FASTA scores: opt: 893, E(): 8.4e-48, (41.85% identity in 344 aa overlap); etc. Contains PS00433 Phosphofructokinase signature. Belongs to the phosphofructokinase family. P9WID7 No intermediary metabolism and respiration P65690 P9WID7 GO:0005945,GO:0005524,GO:0006002,GO:0006096,GO:0046872,GO:0003872 2.7.1.11 P9WID7 NC_000962.3 Mycobrowser_v5 CDS 3549691 3550143 . - 0 Rv3181c Rv3181c Function unknown Conserved protein Rv3181c, (MTV014.25c), len: 150 aa. Conserved protein, with some similarity to other mycobacterium proteins e.g. Q50718|YY07_MYCTU|Rv3407|MT3515|MTCY78.21c (99 aa), FASTA scores: opt: 123, E(): 0.25, (33.7% identity in 89 aa overlap); and O50412|Rv3385c|MTV004.43c (102 aa), FASTA scores: opt: 123, E(): 0.26, (39.7% identity in 68 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WF15 No conserved hypotheticals O53331 P9WF15 NC_000962.3 Mycobrowser_v5 CDS 3371431 3371730 . - 0 Rv3012c gatC Component of the translational apparatus. Furnishes a means for formation of correctly charged GLN-tRNA(GLN) through the transamidation of misacylated GLU- tRNA(GLN) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-GLU-tRNA(GLN) [catalytic activity: ATP + L-glutamyl-tRNA(GLN) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(GLN) + L-glutamate]. Probable glutamyl-tRNA(GLN) amidotransferase (subunit C) GatC (Glu-ADT subunit C) Rv3012c, (MT3092, MTV012.26c), len: 99 aa. Probable gatC, Glu-tRNA-Gln amidotransferase, subunit C, equivalent to O33104|GATC_MYCLE|MLCB637.12 glutamyl-tRNA(GLN) amidotransferase from Mycobacterium leprae (99 aa), FASTA scores: opt: 483, E(): 3.1e-25, (74.75% identity in 99 aa overlap). Also highly similar to other Glu-tRNA-Gln amidotransferases e.g. Q9Z581|GATC_STRCO|SC8D9.10 from Streptomyces coelicolor (98 aa), FASTA scores: opt: 298, E(): 4e-13, (53.7% identity in 95 aa overlap); O06492|GATC_BACSU from B. subtilis (96 aa), FASTA scores: opt: 222, E(): 3.7e-08, (43.15% identity in 95 aa overlap); Q9KF29|BH0665 from Bacillus halodurans (96 aa), FASTA scores: opt: 211, E(): 1.9e-07, (41.05% identity in 95 aa overlap); etc. For more information about function, see citation below. Belongs to the GatC family. P9WN59 No information pathways P64205 P9WN59 GO:0050567,GO:0006450,GO:0006412,GO:0005524 6.3.5.- P9WN59 NC_000962.3 Mycobrowser_v5 CDS 3378329 3378415 . - 0 Rv3018A PE27A Function unknown PE family protein PE27A Rv3018A, len: 28 aa. PE27A, Member of Mycobacterium tuberculosis PE family (see Brennan and Delogu, 2002), most similar to Rv0285 (102 aa), FASTA scores: opt: 147, E(): 3.5e-05, (92.85% identity in 28 aa overlap); etc. Q6MX19 No PE/PPE Q6MX19 Q6MX19 NC_000962.3 Mycobrowser_v5 CDS 3374651 3375664 . - 0 Rv3015c Rv3015c Function unknown Conserved hypothetical protein Rv3015c, (MTV012.29c), len: 337 aa. Conserved hypothetical protein, equivalent to Q9CBR6|ML1706 hypothetical protein from Mycobacterium leprae (337 aa), FASTA scores: opt: 1703, E(): 3.1e-92, (78.05% identity in 337 aa overlap); and (but longer 47 aa) O33101|MLCB637.09 hypothetical 30.0 KDA protein from Mycobacterium leprae (290 aa), FASTA scores: opt: 1564, E(): 2.4e-78, (78.6% identity in 290 aa overlap). Also similar to Q9Z586|SC8D9.05 hypothetical 35.0 KDA protein from Streptomyces coelicolor (331 aa), FASTA scores: opt: 774, E(): 4.7e-38, (43.4% identity in 334 aa overlap); and showing similarity with other proteins e.g. Q39586|METE_CHLRE 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase from Chlamydomonas reinhardtii (814 aa), FASTA scores: opt: 162, E(): 0.048, (27.05% identity in 355 aa overlap). I6YAW3 No conserved hypotheticals O53262 I6YAW3 GO:0009086,GO:0003871 I6YAW3 NC_000962.3 Mycobrowser_v5 CDS 3375758 3376387 . + 0 Rv3016 lpqA Unknown Probable lipoprotein LpqA Rv3016, (MTV012.30), len: 209 aa. Probable lpqA, lipoprotein. Contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. I6Y2A3 No cell wall and cell processes I6Y2A3 I6Y2A3 NC_000962.3 Mycobrowser_v5 CDS 3376939 3378243 . - 0 Rv3018c PPE46 Unknown PPE family protein PPE46 Rv3018c, (MTV012.32c), len: 434 aa. PPE46, Member of PPE family but lacks Gly, Ala rich repeats at C-terminal domain, closest to MTCY261.19. See citation below. Also very similar to following ORF MTV012.35c. Nearly identical in parts to Mycobacterium tuberculosis protein erroneously described as dihydrofolate reductase (X59271|MTFOLA_1) P31500|DYR_MYCTU (214 aa), FASTA scores: opt: 972, E(): 4.4e-42, (80.0% identity in 195 aa overlap); and Z97559|MTCY261_19 from Mycobacterium tuberculosis cosmid (473 aa), FASTA scores: opt: 806, E(): 0; (38.8% identity in 479 aa overlap); and O53268|MTV012.35c from Mycobacterium tuberculosis (358 aa), FASTA scores: opt: 1714, E(): 3.3e-79, (78.3% identity in 355 aa overlap). P9WHY9 No PE/PPE P31500 P9WHY9 P9WHY9 NC_000962.3 Mycobrowser_v5 CDS 3379376 3380452 . - 0 Rv3021c PPE47 Unknown PPE family protein PPE47 Rv3021c, (MTV012.35c), len: 358 aa. PPE47, Member of Mycobacterium tuberculosis PPE family. Should be continuation of upstream ORF MTV012.36c but is frameshifted due to missing base at 36448 in v012. Sequence has been checked but no error apparent. Very similar to neighbouring ORF O53265|MTV012.32c|Rv3018c from Mycobacterium tuberculosis (434 aa), FASTA scores: opt: 1714, E(): 6.6e-770, (78.3% identity in 355 aa overlap) and AAK47430|MT3101 (strongly in the N-terminal part) (310 aa), FASTA scores: opt: 897, E(): 4.5e-37, (66.95% identity in 227 aa overlap). O53268 Yes PE/PPE O53268 O53268 P9WHY7 NC_000962.3 Mycobrowser_v5 CDS 3380679 3380993 . - 0 Rv3022A PE29 Function unknown PE family protein PE29 Rv3022A, len: 104 aa. PE29, Member of the Mycobacterium tuberculosis PE family (see Brennan and Delogu, 2002), similar to many others e.g. Rv0285|AL021930_12 from Mycobacterium tuberculosis (102 aa), FASTA scores: opt: 497, E(): 3e-21, (80.39% identity in 102 aa overlap); etc. Q6MX17 No PE/PPE Q6MX17 Q6MX17 Q6MX17 NC_000962.3 Mycobrowser_v5 CDS 3381375 3382622 . - 0 Rv3023c Rv3023c Required for the transposition of the insertion sequence IS1081. Probable transposase Rv3023c, (MTV012.38c), len: 415 aa. Probable IS1081 transposase. Contains PS01007 Transposases, Mutator family, signature. Similars to P35882|TRA1_MYCTU|Rv1199c|MTCI364.11c and Rv2512c|MTCY07A7.18c transposases for insertion sequence element IS1081 (415 aa), FASTA scores: opt: 2675, E(): 1.8e-162, (100.0% identity in 415 aa overlap). Belongs to the mutator family of transposase. P96354 No insertion seqs and phages P96354 P96354 GO:0004803,GO:0006313,GO:0003677 P96354 NC_000962.3 Mycobrowser_v5 CDS 3382785 3383888 . - 0 Rv3024c trmU Involved in translation mechanisms [catalytic activity: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing 5-methylaminomethyl-2-thiouridylate]. Probable tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase TrmU Rv3024c, (MT3108, MTV012.39c), len: 367 aa. Probable trmU, tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase , equivalent to O33099|TRMU_MYCLE|ML1707|MLCB637.07 probable tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase from Mycobacterium leprae (358 aa), FASTA scores: opt: 2033, E(): 5.5e-116, (85.45% identity in 357 aa overlap). Also highly similar to others e.g. O86583|TRMU_STRCO|SC2A11.22 from Streptomyces coelicolor (376 aa), FASTA scores: opt: 1336, E(): 1e-73, (56.9% identity in 369 aa overlap); BAB49856|MLR2824 from Rhizobium loti (378 aa), FASTA scores: opt: 826, E(): 8.3e-43, (42.35% identity in 359 aa overlap); Q9ZDM1|TRMU_RICPR|RP306 from Rickettsia prowazekii (358 aa), FASTA scores: opt: 800, E(): 3e-41, (40.1% identity in 359 aa overlap); etc. Belongs to the TrmU family. P9WJS5 No information pathways P9WJS5 GO:0005737,GO:0000049,GO:0006400,GO:0016740,GO:0005524 2.8.1.- P9WJS5 NC_000962.3 Mycobrowser_v5 CDS 3378711 3379001 . - 0 Rv3019c esxR Unknown Secreted ESAT-6 like protein EsxR (TB10.3) (ESAT-6 like protein 9) Rv3019c, (MT3104, MTV012.33c), len: 96 aa. EsxR, secreted ESAT-6 like protein (see citations below), most similar to O53693|AAK44525|Rv0288|CFP7|MT0301|MTV035.16 10 KDA antigen CFP7 (low molecular weight protein antigen 7) (CFP-7) from Mycobacterium tuberculosis (95 aa), FASTA scores: opt: 566, E(): 5.1e-31, (84.3% identity in 95 aa overlap). Also similar to Q9CD33|ML2531 possible cell surface protein from Mycobacterium leprae (96 aa), FASTA scores: opt: 472, E(): 8.3e-25, (66.6% identity in 96 aa overlap); O53264|Rv3017c|MTV012.31c putative secreted antigen from Mycobacterium tuberculosis (120 aa), FASTA scores: opt: 321, E(): 9.6e-15, (67.15% identity in 70 aa overlap); Q57165|AAK48357|O84901|X79562|ESAT6|Rv3875|MT3989|MTV027.10 esat6 gene from Mycobacterium tuberculosis strain Erdman (94 aa), FASTA scores: opt: 131, E(): 0.028, (26.1% identity in 88 aa overlap). Belongs to the ESAT6 family. P9WNI9 No cell wall and cell processes 3H6P P9WNI9 P9WNI9 NC_000962.3 Mycobrowser_v5 CDS 3380440 3380682 . - 0 Rv3022c PPE48 Function unknown PPE family protein PPE48 Rv3022c, (MTV012.36c), len: 81 aa. PPE48, Member of M. tuberculosis PPE family with frameshift due to missing bp in codon 82. The ORF continues in downstream MTV012.35c. The sequence has been checked and no errors were detected. Identical to neigbouring ORF O53265|Rv3018c|MTV012.32c (434 aa), FASTA scores: opt: 526, E(): 6.2e-26, (100.0% identity in 81 aa overlap); and O69706|Rv739c|MTV025.087c (77 aa), FASTA scores: opt: 392, E(): 3.4e-18, (72.7% identity in 77 aa overlap). O53268 Yes PE/PPE O53268 O53268 P9WHY7 NC_000962.3 Mycobrowser_v5 CDS 3386074 3386919 . - 0 Rv3027c Rv3027c Acetylation, substrate unknown GCN5-related N-acetyltransferase Rv3027c, (MTV012.42c), len: 281 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain in N-terminal part. See Vetting et al. 2005. Similar, to others e.g. Q9RCY9|SCM10.09c from Streptomyces coelicolor (256 aa), FASTA scores: opt: 498, E(): 7.8e-24, (47.7% identity in 237 aa overlap); BAB50158|MLR3216 from Rhizobium loti (291 aa), FASTA scores: opt: 359, E(): 3.7e-15, (33.35% identity in 246 aa overlap); etc. Start changed since first submission, extended by 25 aa. I6YEZ8 No intermediary metabolism and respiration I6YEZ8 I6YEZ8 NC_000962.3 Mycobrowser_v5 CDS 3388070 3388870 . - 0 Rv3029c fixA The electron transfer flavoprotein serves as a specific electron acceptor for other dehydrogenases. It transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Probable electron transfer flavoprotein (beta-subunit) FixA (beta-ETF) (electron transfer flavoprotein small subunit) (ETFSS) Rv3029c, (MTV012.44c), len: 266 aa. Probable fixA (alternate gene name: etfB), electron transfer flavoprotein (beta-subunit). Equivalent of O33095|ETFB_MYCLE|FixA|MLCB637.03 electron transfer flavoprotein from Mycobacterium leprae (266 aa), FASTA scores: opt: 1603, E(): 7.6e-87, (95.1% identity in 266 aa overlap). Also highly similar to others e.g. Q9K417|SCG22.28c from Streptomyces coelicolor (262 aa), FASTA scores: opt: 860, E(): 2.3e-43, (52.4% identity in 263 aa overlap); O85691|ETFB_MEGEL from Megasphaera elsdenii (270 aa), FASTA scores: opt: 548, E(): 4.2e-25, (35.15% identity in 273 aa overlap); etc. Also highly similar in particular to Q9KHD0|NONH flavoprotein reductase from Streptomyces griseus subsp. griseus (this one is required for macrotetrolide biosynthesis in Streptomyces griseus) (261 aa), FASTA scores: opt: 867, E(): 8.8e-44, (54.0% identity in 263 aa overlap). Belongs to the Etf beta-subunit / FixA family. P9WNG7 No intermediary metabolism and respiration P64097 P9WNG7 GO:0022900,GO:0006810,GO:0009055 P9WNG7 NC_000962.3 Mycobrowser_v5 CDS 3389101 3389925 . + 0 Rv3030 Rv3030 Function unknown Conserved protein Rv3030, (MTV012.45), len: 274 aa. Conserved protein, equivalent to O33094|MLCB637.02c|ML1713 hypothetical 30.8 KDa protein from Mycobacterium leprae (280 aa), FASTA scores: opt: 1388, E(): 5.5e-83, (78.2% identity in 280 aa overlap). N-terminus has similarity to hypothetical proteins from a number of organisms and to Q54303|EMBL:X86780|RAPM methyltransferase from Streptomyces hygroscopicus (317 aa), FASTA scores: opt: 191, E(): 3.6e-05, (35.65% identity in 101 aa overlap). P9WJZ1 No conserved hypotheticals O53277 P9WJZ1 GO:0008168,GO:0008152 P9WJZ1 NC_000962.3 Mycobrowser_v5 CDS 3391534 3392778 . + 0 Rv3032 Rv3032 Involved in the synthesis of glycogen and 6-O-methylglucosyl-containing lipopolysaccharides (MGLP) Alpha (1->4) glucosyltransferase Rv3032, (MTV012.47), len: 414 aa. Alpha (1->4) glucosyltransferase (See Stadthagen et al., 2007). Equivalent to Q9CBR3|ML1715 putative transferase from Mycobacterium leprae (438 aa), FASTA scores: opt: 2456, E(): 7.3e-145, (87.9% identity in 414 aa overlap). Also similar to hypothetical proteins and various transferases e.g. P73369|SLL1971 hypothetical 46.2 KDA protein from Synechocystis sp. strain PCC 6803 (404 aa), FASTA scores: opt: 584, E(): 7.3e-29, (34.5% identity in 400 aa overlap); Q9Z5B7|SC2G5.06 putative transferase from Streptomyces coelicolor (406 aa), FASTA scores: opt: 509, E(): 3.3e-24, (35.9% identity in 413 aa overlap); Q9UZA1|PAB0827 galactosyltransferase (LPS biosynthesis RFBU related protein) from Pyrococcus abyssi (371 aa), FASTA scores: opt: 381, E(): 2.6e-16, (26.75% identity in 404 aa overlap); etc. P9WMY9 No intermediary metabolism and respiration O53279 P9WMY9 GO:0016740,GO:0009058 2.-.-.- P9WMY9 NC_000962.3 Mycobrowser_v5 CDS 3393380 3393928 . + 0 Rv3033 Rv3033 Unknown Unknown protein Rv3033, (MTV012.48), len: 182 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). I6YAY5 No conserved hypotheticals I6YAY5 NC_000962.3 Mycobrowser_v5 CDS 3394019 3394921 . - 0 Rv3034c Rv3034c Function unknown; possibly involved in cellular metabolism. Possible transferase Rv3034c, (MTV012.49c), len: 300 aa. Possible transferase (2.-.-.-), equivalent to AAK47449|MT3119 Hexapeptide transferase family protein from M. tuberculosis strain CDC1551 but N-terminus shorter 39 residues (262 aa), FASTA scores: opt: 1773, E(): 4.7e-105, (100.0% identity in 262 aa overlap). Similar to Q9CBR1|ML1719 from Mycobacterium leprae but also shorter in N-terminus (245 aa), FASTA scores: opt: 1549, E(): 6.6e-91, (90.6% identity in 244 aa overlap). Some weakly similarity with other transferases (C-terminal part shows some similarity to acetyltransferase from Methanococcus jannaschii (214 aa)). Alternative start possible at 3395077 but codon usage not as good. O53281 No intermediary metabolism and respiration O53281 GO:0016740 2.-.-.- O53281 NC_000962.3 Mycobrowser_v5 CDS 3395379 3396461 . + 0 Rv3035 Rv3035 Function unknown Conserved protein Rv3035, (MTV012.50), len: 360 aa. Conserved protein, equivalent to Q9CBR0|ML1720 hypothetical protein from Mycobacterium leprae (364 aa), FASTA scores: opt: 1963, E(): 1.4e-108, (75.8% identity in 363 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). I6XFZ8 No conserved hypotheticals O53282 I6XFZ8 I6XFZ8 NC_000962.3 Mycobrowser_v5 CDS 3408022 3408306 . - 0 Rv3047c Rv3047c Unknown Hypothetical protein Rv3047c, (MTV012.62c), len: 94 aa. Hypothetical unknown protein. I6X642 No conserved hypotheticals I6X642 NC_000962.3 Mycobrowser_v5 CDS 3411217 3411957 . - 0 Rv3050c Rv3050c Involved in transcriptional mechanism Probable transcriptional regulatory protein (probably AsnC-family) Rv3050c, (MTV012.65c), len: 246 aa. Probable transcriptional regulatory protein TetR-family, equivalent but shorter to Q9CBQ1|ML1733 from Mycobacterium leprae (275 aa), FASTA scores: opt: 1381,(E): 2.7e-79, (86.25% identity in 240 aa overlap); AAK44712|MT0489 from Mycobacterium tuberculosis strain CDC1551 (256 aa), FASTA scores: opt: 328,(E): 1.8e-13, (30.75% identity in 234 aa overlap); etc. Also some similarity to O53757|Rv0472c|MTV038.16c. Alternative starts possible at 68052 or 67923. Has potential helix-turn-helix motif at positons 51-72. I6XG13 No regulatory proteins O53295 I6XG13 GO:0006350,GO:0003700,GO:0006355 I6XG13 NC_000962.3 Mycobrowser_v5 CDS 3401055 3401918 . - 0 Rv3041c Rv3041c Thought to be involved in active transport of undetermined substrate (possibly iron) across the membrane. Responsible for energy coupling to the transport system. Probable conserved ATP-binding protein ABC transporter Rv3041c, (MTV012.56c), len: 287 aa. Probable conserved ATP-binding protein ABC transporter (see citation below), equivalent to Q9CBQ7|ML1726 putative ABC transporter protein ATP-binding protein from Mycobacterium leprae (305 aa), FASTA scores: opt: 1576, E(): 8.6e-85, (83.4% identity in 289 aa overlap). Also similar to other putative ATP-binding proteins ABC transporters e.g. Q9X9Z4|SCI5.06C from Streptomyces coelicolor (265 aa), FASTA scores: opt: 893, E(): 4.8e-45, (53.3% identity in 257 aa overlap); Q9L156|SC5C11.16c from Streptomyces coelicolor (279 aa), FASTA scores: opt: 680, E(): 1.3e-32, (45.4% identity in 271 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters). I6YF11 No cell wall and cell processes O53288 I6YF11 GO:0016887,GO:0005524 I6YF11 NC_000962.3 Mycobrowser_v5 CDS 3401933 3403162 . - 0 Rv3042c serB2 Generates serine from phosphoserine [catalytic activity: phosphoserine + H(2)O = serine + phosphate]. Probable phosphoserine phosphatase SerB2 (PSP) (O-phosphoserine phosphohydrolase) (pspase) Rv3042c, (MTV012.57c), len: 409 aa. Probable serB2, Phosphoserine phosphatase, equivalent to Q9CBQ6|ML1727 putative phosphoserine phosphatase from Mycobacterium leprae (411 aa), FASTA scores: opt: 2173, E(): 1.3e-117, (86.3% identity in 408 aa overlap). Also similar to other e.g. Q9S281|SCI28.02 from Streptomyces coelicolor (410 aa), FASTA scores: opt: 1209, E(): 3e-62, (51.75% identity in 400 aa overlap); Q9HUK|PA4960 from Pseudomonas aeruginosa (429 aa), FASTA scores: opt: 704, E(): 3.1e-33, (40.95% identity in 393 aa overlap); O28142|SERB_ARCTU|AF2138 from Archaeoglobus fulgidus (344 aa), FASTA scores: opt: 671, E(): 2e-31, (37.25% identity in 325 aa overlap); and P06862|SERB_ECOLI (322 aa), FASTA scores: opt: 628, E(): 5.7e-29, (46.8% identity in 235 aa overlap). Belongs to the SerB family. O53289 No intermediary metabolism and respiration O53289 O53289 GO:0016597,GO:0004647,GO:0006564 3.1.3.3 O53289 NC_000962.3 Mycobrowser_v5 CDS 3403200 3404921 . - 0 Rv3043c ctaD Cytochrome C oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome C are transferred via the copper a center of subunit 2 and heme a of subunit 1 to the bimetallic center formed by heme A3 and copper B [catalytic activity: 4 ferrocytochrome C + O(2) = 2 H(2)O + 4 ferricytochrome C]. Probable cytochrome C oxidase polypeptide I CtaD (cytochrome AA3 subunit 1) Rv3043c, (MTV012.58c), len: 573 aa. Probable ctaD, integral membrane cytochrome C oxidase polypeptide I, equivalent to Q9CBQ5|ML1728 from Mycobacterium leprae (574 aa), FASTA scores: opt: 3738, E(): 3.8e-216, (95.4% identity in 566 aa overlap). Also similar to other cytochrome C oxidases polypeptide I e.g. Q9AEL9|CTAD from Corynebacterium glutamicum (Brevibacterium flavum) (584 aa), FASTA scores: opt: 3065, E(): 6.8e-176, (72.65% identity in 567 aa overlap); Q9X813|SC6G10.28c from Streptomyces coelicolor (578 aa), FASTA scores: opt: 2888, E(): 2.6e-165, (71.7% identity in 544 aa overlap); Q9K451|CTAD from Streptomyces coelicolor (573 aa), FASTA scores: opt: 2757, E(): 1.8e-157, (70.2% identity in 537 aa overlap). Contains PS00077 Cytochrome c oxidase subunit I, copper B binding region signature. Belongs to the heme-copper respiratory oxidase family. P9WP71 No intermediary metabolism and respiration P63852 P9WP71 GO:0005507,GO:0004129,GO:0009055,GO:0022900,GO:0020037,GO:0016021,GO:0005886,GO:0070469,GO:0006810,GO:0009060 1.9.3.1 P9WP71 NC_000962.3 Mycobrowser_v5 CDS 3415435 3415989 . - 0 Rv3054c Rv3054c Function unknown Conserved hypothetical protein Rv3054c, (MTCY22D7.28), len: 184 aa. Conserved hypothetical protein, similar to Q9RD22|SCM1.21 putative secreted protein from Streptomyces coelicolor (187 aa), FASTA scores: opt: 651, E(): 1.5e-33, (56.8% identity in 175 aa overlap). Also shares similarity with other hypothetical proteins and Chromate reductases e.g. AAK56853|CHRR from Pseudomonas putida (186 aa), FASTA scores: opt: 339, E(): 3.3e-14, (38.75% identity in 160 aa overlap). Contains aminotransferases class-II pyridoxal-phosphate attachment site (PS00599) near C-terminus. P95105 No conserved hypotheticals P95105 P95105 P95105 NC_000962.3 Mycobrowser_v5 CDS 3406285 3407325 . + 0 Rv3045 adhC Generates aldehyde or ketone from alcohol [catalytic activity: alcohol + NADP(+) = aldehyde or ketone + NADPH]. Probable NADP-dependent alcohol dehydrogenase AdhC Rv3045, (MTV012.60), len: 346 aa. Probable adhC, NADP-dependent alcohol dehydrogenase, equivalent to Q9CBQ3|ADHA|ML1730 alcohol dehydrogenases from Mycobacterium leprae (362 aa), FASTA scores: opt: 1982, E(): 1.3e-111, (85.85% identity in 346 aa overlap); Q9AE96|ADHC from Mycobacterium smegmatis (348 aa), FASTA scores: opt: 1808, E(): 3.4e-101, (78.95% identity in 347 aa overlap); Q9EWF1|SCK13.33c putative dehydrogenase from Streptomyces coelicolor (346 aa), FASTA scores: opt: 1508, E(): 3.3e-83, (64.45% identity in 346 aa overlap); O06007|ADHA from Bacillus subtilis (349 aa), FASTA scores: opt: 1412, E(): 1.9e-77, (61.8% identity in 335 aa overlap); etc. Contains PS00059 Zinc-containing alcohol dehydrogenases signature. Belongs to the zinc-containing alcohol dehydrogenase family. High similarity with other bacterial ADH'S. P9WQC5 No intermediary metabolism and respiration P0A4X0 P9WQC5 GO:0055114,GO:0008270,GO:0008106 1.1.1.2 P9WQC5 NC_000962.3 Mycobrowser_v5 CDS 3408404 3409378 . - 0 Rv3048c nrdF2 Involved in the DNA replication pathway. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides, precursors that are necessary for DNA synthesis [catalytic activity: 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H(2)O = ribonucleoside diphosphate + reduced thioredoxin]. Ribonucleoside-diphosphate reductase (beta chain) NrdF2 (ribonucleotide reductase small subunit) (R2F protein) Rv3048c, (MTV012.63c), len: 324 aa. NrdF2, ribonucleoside-diphosphate reductase, beta chain (see citation below), equivalent to Q9CBQ2|RIR2_MYCL|NRDF|ML1731 ribonucleoside-diphosphate reductase beta chain from Mycobacterium leprae (325 aa), FASTA scores: opt: 2009, E(): 1.3e-123, (93.5% identity in 324 aa overlap). Also similar to other ribonucleoside-diphosphate reductases e.g. Q9XD62|NRDF from Corynebacterium glutamicum (Brevibacterium flavum) (334 aa), FASTA scores: opt: 1648, E(): 4.2e-100, (78.35% identity in 314 aa overlap); O69274|NRDF from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (329 aa), FASTA scores: opt: 1626, E(): 1.1e-98, (75.3% identity in 320 aa overlap); P37146|NRDF|B2676 from Escherichia coli (319 aa), FASTA scores: opt: 1569, E(): 5.7e-95, (71.3% identity in 317 aa overlap). Contains PS00368 Ribonucleotide reductase small subunit signature. Belongs to the ribonucleoside diphosphate reductase small chain family. Cofactor: binds 2 iron ions (by similarity). Note that previously known as nrdG. P9WH71 No information pathways 1UZR Q50549 P9WH71 GO:0009186,GO:0055114,GO:0004748,GO:0046914,GO:0006260 1.17.4.1 P9WH71 NC_000962.3 Mycobrowser_v5 CDS 3409509 3411083 . - 0 Rv3049c Rv3049c Function unknown; involved in cellular metabolism. Probable monooxygenase Rv3049c, (MTV012.64c), len: 524 aa. Probable monooxygenase, similar to several monooxygenases e.g. Q9I3H5|PA1538 probable flavin-containing monooxygenase from Pseudomonas aeruginosa (527 aa), FASTA scores: opt: 1577, E(): 3.9e-90, (47.3% identity in 501 aa overlap); Q9RKB5|SCE87.23c monooxygenase from Streptomyces coelicolor (519 aa), FASTA scores: opt: 1522, E(): 9.8e-87, (47.4% identity in 485 aa overlap); Q9I218|PA2097 probable flavin-binding monooxygenase from Pseudomonas aeruginosa (491 aa), FASTA scores: opt: 1366, E(): 4.2e-77, (43.75% identity in 489 aa overlap); etc. Also similar to Q10532|Rv0892|Y892_MYCTU|MT0916|MTCY31.20 probable monooxygenase from Mycobacterium tuberculosis strain H37Rv (495 aa), FASTA scores: opt: 1147, E(): 1.5e-63, (38.0% identity in 479 aa overlap). I6Y2E2 No intermediary metabolism and respiration O53294 I6Y2E2 GO:0050661,GO:0004499,GO:0055114,GO:0050660 1.-.-.- I6Y2E2 NC_000962.3 Mycobrowser_v5 CDS 3412085 3414166 . - 0 Rv3051c nrdE Involved in the DNA replication pathway. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides, precursors that are necessary for DNA synthesis. [catalytic activity: 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H(2)O = ribonucleoside diphosphate + reduced thioredoxin]. Ribonucleoside-diphosphate reductase (alpha chain) NrdE (ribonucleotide reductase small subunit) (R1F protein) Rv3051c, (MTV012.66c), len: 693 aa. NrdE, ribonucleotide-diphosphate reductase, alpha chain (see citations below), equivalent to Q9CBQ0|NRDE|ML1734 from Mycobacterium leprae (693 aa), FASTA scores: opt: 4259,E(): 0, (93.2% identity in 693 aa overlap). Similar to other Ribonucleoside-diphosphate reductases e.g. Q9XD63|NRDE from Corynebacterium glutamicum (Brevibacterium flavum) (707 aa), FASTA scores: opt: 3683,E(): 0, (79.35% identity in 693 aa overlap); O69273|NRDE from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (720 aa), FASTA scores: opt: 3555, E(): 1.7e-214, (76.1% identity in 694 aa overlap); P39452|NRDE|B2675 from Escherichia coli (713 aa), FASTA scores: opt: 3430, E(): 1.1e-206, (73.6% identity in 693 aa overlap); etc. Equivalent to AAK47468|MT3137 from Mycobacterium tuberculosis strain CDC1551 (725 aa) but shorter in N-terminus. Contains PS00089 Ribonucleotide reductase large subunit signature. Belongs to the ribonucleoside diphosphate reductase large chain family. P9WH75 No information pathways P0A5W8 P9WH75 GO:0006260,GO:0055114,GO:0005515,GO:0004748,GO:0005971,GO:0005524 1.17.4.1 P9WH75 NC_000962.3 Mycobrowser_v5 CDS 3416705 3417745 . + 0 Rv3056 dinP Thought to be involved in DNA metabolism and mutagenesis [catalytic activity: N deoxynucleoside triphosphate = N diphosphate + {DNA}N]. Possible DNA-damage-inducible protein P DinP (DNA polymerase V) (pol IV 2) (DNA nucleotidyltransferase (DNA-directed)) Rv3056, (MTCY22D7.25c, MT3142), len: 346 aa. Possible dinP (alternate gene name: dinB2), DNA-damage-inducible protein (DNA polymerase V) (see citations below), similar to others e.g. AAK45855|MT1589 from Mycobacterium tuberculosis strain CDC1551 (485 aa), FASTA scores: opt: 620, E(): 6.1e-32, (37.2% identity in 344 aa overlap); BAB49140|MLR1877 from Rhizobium loti (Mesorhizobium loti) (415 aa), FASTA scores: opt: 533, E(): 1.8e-26, (34.35% identity in 358 aa overlap); and BAB54888|MLL9709 from Rhizobium loti (Mesorhizobium loti) (361 aa), FASTA scores: opt: 532, E(): 1.8e-26, (35.35% identity in 348 aa overlap). Extensive similarity to proteins induced by DNA damage such as dinP, mucB, umuC. Belongs to the DNA polymerase type-Y family. P9WNT1 No information pathways P63987 P9WNT1 GO:0006261,GO:0003887,GO:0005737,GO:0003684,GO:0000287,GO:0006281 2.7.7.7 P9WNT1 NC_000962.3 Mycobrowser_v5 CDS 3425584 3427107 . + 0 Rv3062 ligB This protein seals during DNA replication, DNA recombination and DNA repair NICKS in double-stranded DNA [catalytic activity: ATP + (deoxyribonucleotide)(N) + (deoxyribonucleotide)(M) = AMP + pyrophosphate + (deoxyribonucleotide)(N+M)]. Probable ATP-dependent DNA ligase LigB (polydeoxyribonucleotide synthase [ATP]) (polynucleotide ligase [ATP]) (sealase) (DNA repair protein) (DNA joinase) Rv3062, (MTCY22D7.19c), len: 507 aa. Probable ligB, DNA ligase ATP-dependent (see citation below), highly similar to numerous archaebacterial and eukaryotic polynucleotide DNA ligases, e.g. Q9FCB1|DNLI_STRCO|LIG|2SCG58.02 from Streptomyces coelicolor (512 aa), FASTA scores: opt: 1677, E(): 2.5e-90, (55.65% identity in 512 aa overlap); Q9HR35|DNLI_HALN1|LIG|VNG0881G from Halobacterium sp. strain NRC-1 (561 aa), FASTA scores: opt: 985, E(): 5.6e-50, (42.25% identity in 440 aa overlap); Q9V185|DNLI_PYRAB|LIG|PAB2002 from Pyrococcus abyssi (559 aa), FASTA scores: opt: 978, E(): 1.4e-49, (39.05% identity in 443 aa overlap); etc. Also similar to Rv3731|MTV025.079|LIGC possible DNA ligase from M. tuberculosis (358 aa). Similarity at N-terminus is poor so first start codon was taken. Contains (PS00697) ATP-dependent DNA ligase AMP-binding site signature, and (PS00017) ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-dependent DNA ligase family. P9WNV5 No information pathways P95096 P9WNV5 GO:0007049,GO:0003677,GO:0003910,GO:0006310,GO:0006281,GO:0006260,GO:0051301,GO:0046872,GO:0005524 6.5.1.1 P9WNV5 NC_000962.3 Mycobrowser_v5 CDS 3715777 3716994 . + 0 Rv3330 dacB1 Involved in peptidoglycan synthesis (at final stages). Hydrolyzes the bound D-alanyl-D-alanine [catalytic activity: D-alanyl-D-alanine + H(2)O = 2 D-alanine]. Probable penicillin-binding protein DacB1 (D-alanyl-D-alanine carboxypeptidase) (DD-peptidase) (DD-carboxypeptidase) (PBP) (DD-transpeptidase) (serine-type D-ala-D-ala carboxypeptidase) (D-amino acid hydrolase) Rv3330, (MTV016.30), len: 405 aa. Probable dacB1, D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein), equivalent to Mycobacterium leprae proteins Q9CCM2|ML0691 putative D-alanyl-D-alanine carboxypeptidase (411 aa), FASTA scores: opt: 2066, E(): 2.5e-102, (77.15% identity in 416 aa overlap); Q49917|L308_F1_36 (228 aa), FASTA scores: opt: 1241, E(): 7.9e-59, (78.9% identity in 232 aa overlap) (note that this protein corresponds to C-terminal part of the putative protein encoded by Rv3330, aa 174-405); and Q49921|PBPC (182 aa), FASTA scores: opt: 736, E(): 3.7e-32, (73.95% identity in 169 aa overlap) (note that this protein corresponds to N-terminal part of the putative protein encoded by Rv3330, aa 1-158); note L308_F1_36 (228 aa) and PBPC (182 aa) are two consecutive Mycobacterium leprae ORFs. Also similar to others e.g. Q9FC34|SC4G1.16c putative D-alanyl-D-alanine carboxypeptidase from Streptomyces coelicolor (413 aa), FASTA scores: opt: 572, E(): 3.4e-23, (33.75% identity in 382 aa overlap); P35150|DACB_BACSU penicillin-binding protein 5* precursor (D-alanyl-D-alanine carboxypeptidase) from Bacillus subtilis (382 aa), FASTA scores: opt: 422, E(): 2.8e-15, (31.3% identity in 249 aa overlap); Q9K8X5|DACB|BH2877 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein) from Bacillus halodurans (395 aa), FASTA scores: opt: 421, E(): 3.2e-15, (31.95% identity in 241 aa overlap); etc. Also similar to Mycobacterium tuberculosis Q10828|Rv2911|MTCY274.43 probable penicillin-binding protein (belongs to peptidase family S11; also known as the D-alanyl-D-alanine carboxypeptidase 1 family) (291 aa), FASTA scores: opt: 746, E(): 1.6e-32, (47.0% identity in 266 aa overlap). Has hydrophobic stretches at both N- and C-termini. Certainly membrane-bound protein. Belongs to peptidase family S11; also known as the D-alanyl-D-alanine carboxypeptidase 1 family. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). O53380 No cell wall and cell processes O53380 O53380 GO:0009002,GO:0006508 3.4.16.4 O53380 NC_000962.3 Mycobrowser_v5 CDS 3421741 3423213 . - 0 Rv3060c Rv3060c Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably GntR-family) Rv3060c, (MTCY22D7.21), len: 490 aa. Probable transcriptional regulatory protein, showing reasonable similarity to several members of the GntR family e.g. BAB54431|MLL8575 from Rhizobium loti (Mesorhizobium loti) (247 aa), FASTA scores: opt: 274, E(): 3.5e-10, (30.35% identity in 224 aa overlap); P96570|ESMR from Burkholderia cepacia (Pseudomonas cepacia) (277 aa), FASTA scores: opt: 229, E(): 2.8e-07, (25.85% identity in 240 aa overlap); Q9S276|SCI28.07 from Streptomyces coelicolor (230 aa), FASTA scores: opt: 211, E(): 3.4e-06, (27.25% identity in 220 aa overlap); etc. Seems to have two domains: residues 1-260 resemble UxuR, and 260-490 resemble PdhR, ExuR, etc. Contains bacterial regulatory proteins, GntR family signature (PS00043). Helix-turn-helix motif (+3.13 SD) at aa 38-59. Seems to belong to the GntR family of transcriptional regulators. P95098 No regulatory proteins P95098 P95098 GO:0006355,GO:0006350,GO:0003700,GO:0005622 P95098 NC_000962.3 Mycobrowser_v5 CDS 3436329 3436685 . - 0 Rv3073c Rv3073c Function unknown Conserved hypothetical protein Rv3073c, (MTCY22D7.08), len: 118 aa. Conserved hypothetical protein, highly similar to other e.g. Q9F3D7|SC2H2.18 from Streptomyces coelicolor (119 aa), FASTA scores: opt: 399, E(): 2.5e-20, (53.05% identity in 115 aa overlap); Q9K4K9|SC5F8.15c from Streptomyces coelicolor (117 aa), FASTA scores: opt: 334, E(): 6e-16, (49.1% identity in 112 aa overlap); Q9HKD5|TA0666 from Thermoplasma acidophilum (134 aa), FASTA scores: opt: 334, E(): 6.7e-16, (42.35% identity in 111 aa overlap); BAB53507|MLL7394 from Rhizobium loti (Mesorhizobium loti) (120 aa), FASTA scores: opt: 309, E(): 3e-14, (43.65% identity in 110 aa overlap); etc. P9WL11 No conserved hypotheticals P65063 P9WL11 NC_000962.3 Mycobrowser_v5 CDS 3488569 3489063 . + 0 Rv3123 Rv3123 Unknown Hypothetical protein Rv3123, (MTCY164.33), len: 164 aa. Hypothetical unknown protein, but N-terminus shares weak similarity with N-terminal part of O93439|CMESO-1 BHLH transcription factor from Gallus gallus (Chicken) (287 aa), FASTA scores: opt: 129, E(): 0.81, (38.75% identity in 80 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07034 No conserved hypotheticals O07034 NC_000962.3 Mycobrowser_v5 CDS 3541443 3542045 . - 0 Rv3173c Rv3173c Possibly involved in transcriptional mechanism (probably repression). Probable transcriptional regulatory protein (probably TetR/AcrR-family) Rv3173c, (MTV014.17c), len: 200 aa. Probable transcriptional regulatory protein TetR family, similar to several bacterial putative regulatory proteins e.g. Q9EWI2|SC7H9.14 from Streptomyces coelicolor (195 aa), FASTA scores: opt: 319, E(): 1.7e-13, (34.55% identity in 195 aa overlap); O85695|3SCF60.04 from Streptomyces lividans and Streptomyces coelicolor (192 aa), FASTA scores: opt: 297, E(): 4.3e-12, (37.45% identity in 187 aa overlap); BAB50853|MLR4117 from Rhizobium loti (Mesorhizobium loti) (205 aa), FASTA scores: opt: 280, E(): 5.5e-11, (31.45% identity in 194 aa overlap); BAB53760|MLL8133 from Rhizobium loti (Mesorhizobium loti) (194 aa), FASTA scores: opt: 270, E(): 2.3e-10, (34.05% identity in 185 aa overlap); etc. Also similar to other regulators from Mycobacterium tuberculosis e.g. P96839|Rv3557c|MTCY06G11.04c (200 aa), FASTA scores: opt: 154, E(): 0.0013, (38.8% identity in 80 aa overlap). Contains probable helix-turn-helix motif from aa 39 to 60 (Score 1251, +3.45 SD). Similar to the TetR/AcrR family of transcriptional regulators. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53323 No regulatory proteins O53323 O53323 GO:0006350,GO:0003700,GO:0006355 O53323 NC_000962.3 Mycobrowser_v5 CDS 3429825 3430250 . - 0 Rv3064c Rv3064c Unknown Probable conserved integral membrane protein Rv3064c, (MTCY22D7.17), len: 141 aa. Probable conserved integral membrane protein, similar to many e.g. Q9KY40|SCC8A.08 putative integral membrane protein from Streptomyces coelicolor (153 aa), FASTA scores: opt: 391, E(): 2.4e-18, (48.45% identity in 130 aa overlap); Q9K461|SC2H12.23c putative integral membrane protein from Streptomyces coelicolor (151 aa), FASTA scores: opt: 339, E(): 5.1e-15, (46.7% identity in 124 aa overlap); BAB48975|MLR1652 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (130 aa), FASTA scores: opt: 319, E(): 8.7e-14, (41.45% identity in 123 aa overlap); Q9JR31|NMA2196|NMB0291 conserved hypothetical inner membrane protein from Neisseria meningitidis serogroup a and B (132 aa), FASTA scores: opt: 303, E(): 9.4e-13, (43.65% identity in 126 aa overlap); etc. I6XG31 No cell wall and cell processes O86323 I6XG31 NC_000962.3 Mycobrowser_v5 CDS 3550374 3550718 . + 0 Rv3182 Rv3182 Function unknown Conserved hypothetical protein Rv3182, (MTV014.26), len: 114 aa. Hypothetical protein, with some similarity to other hypothetical bacterial proteins e.g. O53468|Rv2022c|MTV018.09c from M. tuberculosis (201 aa), FASTA scores: opt: 335, E(): 3.6e-16, (51.9% identity in 104 aa overlap); and Q9L3R6|ORF119 from Anabaena sp. strain PCC 7120 (119 aa), FASTA scores: opt: 250, E(): 1.6e-10, (42.1% identity in 95 aa overlap). Equivalent to AAK47614 from Mycobacterium tuberculosis strain CDC1551 (94 aa) but longer 20 aa. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53332 No conserved hypotheticals O53332 O53332 O53332 NC_000962.3 Mycobrowser_v5 CDS 3427243 3429519 . + 0 Rv3063 cstA Peptide utilization during carbon starvation. Probable carbon starvation protein A homolog CstA Rv3063, (MTCY22D7.18c), len: 758 aa. Probable cstA, integral membrane starvation-induced stress response protein, similar to other e.g. P15078|CSTA_ECOLI|B0598 from Escherichia coli strain K12 (701 aa), FASTA scores: opt: 2357, E(): 9.5e-137, (51.25% identity in 712 aa overlap); AAG54933|CSTA from Escherichia coli strain O157:H7 EDL933 (701 aa), FASTA scores: opt: 2356, E(): 1.1e-136, (51.1% identity in 712 aa overlap); etc. Predicted to be membrane associated. Similarity suggests start at GTG at 16801 in Y22D7 but no RBS obvious so TBParse-predicted start at 16881 taken. Belongs to the CstA family. P9WP47 No virulence, detoxification, adaptation P95095 P9WP47 GO:0016021,GO:0005886,GO:0009267 P9WP47 NC_000962.3 Mycobrowser_v5 CDS 3430707 3431315 . + 0 Rv3066 Rv3066 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably DeoR-family) Rv3066, (MTCY22D7.15c), len: 202 aa. Probable transcriptional regulatory protein deoR-family, with some similarity to transcriptional regulators and hypothetical proteins, e.g. Q9X9V5|SCI7.35c hypothetical 21.1 KDA protein from Streptomyces coelicolor (197 aa), FASTA scores: opt: 398, E(): 5.7e-19, (40.3% identity in 191 aa overlap); AAG55222|Z1073 putative DeoR-type transcriptional regulator from Escherichia coli strain O157:H7 EDL933 (178 aa), FASTA scores: opt: 257, E(): 7.9e-10, (28.4% identity in 176 aa overlap); Q9HXU1|PA3699 probable transcriptional regulator (TetR/AcrR family) from Pseudomonas aeruginosa (237 aa), FASTA scores: opt: 229, E(): 6.7e-08, (32.1% identity in 187 aa overlap); etc. I6X658 No regulatory proteins P95092 I6X658 GO:0006350,GO:0003700,GO:0006355 I6X658 NC_000962.3 Mycobrowser_v5 CDS 3431428 3431838 . + 0 Rv3067 Rv3067 Function unknown Conserved hypothetical protein Rv3067, (MTCY22D7.14c), len: 136 aa. Conserved hypothetical protein, weakly similar to other mycobacterium proteins e.g. O53953|Rv1804c|MTV049.26c (108 aa), FASTA scores: opt: 183, E(): 0.00053, (36.6% identity in 82 aa overlap); O07222|Rv1810|MTCY16F9.04c (118 aa), FASTA scores: opt: 149, E(): 0.05, (30.95% identity in 84 aa overlap). Has hydrophobic stretch at N-terminus. Start chosen on basis of codon usage but upstream ATG also possible. I6YB21 No conserved hypotheticals P95091 I6YB21 NC_000962.3 Mycobrowser_v5 CDS 3433692 3434090 . + 0 Rv3069 Rv3069 Unknown Probable conserved transmembrane protein Rv3069, (MTCY22D7.12c), len: 132 aa. Probable conserved transmembrane protein, similar to several hypothetical and CRCB bacterial proteins e.g. Q9A6V2|CC1981 CRCB protein (see citation below; seems to be involved in camphor resistance and chromosome condensation, promoting or protecting chromosome folding) from Caulobacter crescentus (127 aa), FASTA scores: opt: 275, E(): 1.6e-11, (41.1% identity in 124 aa overlap); Q9FC39|SC4G1.10 putative integral membrane protein from Streptomyces coelicolor (154 aa), FASTA scores: opt: 258, E(): 2.5e-10, (42.15% identity in 121 aa overlap); Q9V0X2|PAB1925 CRCB protein (see citation below) from Pyrococcus abyssi (123 aa), FASTA scores: opt: 256, E(): 2.8e-10, (39.8% identity in 113 aa overlap); O59171|PH1502 hypothetical 13.6 KDA protein from Pyrococcus horikoshii (123 aa), FASTA scores: opt: 249, E(): 8.2e-10, (38.65% identity in 119 aa overlap); etc. P9WP63 No cell wall and cell processes P63862 P9WP63 GO:0005886,GO:0016021 P9WP63 NC_000962.3 Mycobrowser_v5 CDS 3434087 3434467 . + 0 Rv3070 Rv3070 Unknown Probable conserved integral membrane protein Rv3070, (MTCY22D7.11c), len: 126 aa. Probable conserved integral membrane protein, similar to several hypothetical and CRCB bacterial proteins e.g. Q9FC37|SC4G1.12 putative integral membrane protein from Streptomyces coelicolor (124 aa), FASTA scores: opt: 280, E(): 3.1e-11, (45.3% identity in 117 aa overlap); O25823|HP1225 conserved hypothetical integral membrane protein from Helicobacter pylori (Campylobacter pylori) (130 aa), FASTA scores: opt: 225, E(): 1e-07, (33.35% identity in 123 aa overlap); O07590|YHDU hypothetical 12.4 KDA protein from Bacillus subtilis (118 aa), FASTA scores: opt: 224, E(): 1.1e-07, (37.85% identity in 111 aa overlap); Q9KVS9|VC0060 CRCB protein (see Hu et al., 1996; seems involved in camphor resistance and chromosome condensation, promoting or protecting chromosome folding) from Vibrio cholera (126 aa), FASTA scores: opt: 221, E(): 1.8e-07, (33.35% identity in 126 aa overlap); etc. P9WP61 No cell wall and cell processes P63864 P9WP61 GO:0005886,GO:0016021 P9WP61 NC_000962.3 Mycobrowser_v5 CDS 3434464 3435573 . + 0 Rv3071 Rv3071 Function unknown Conserved hypothetical protein Rv3071, (MTCY22D7.10c), len: 369 aa. Conserved hypothetical protein, weakly similar in N-terminus of Q9A4V0|CC2725 hypothetical protein CC2725 from Caulobacter crescentus (113 aa), FASTA scores: opt: 141, E(): 0.031, (27.6% identity in 105 aa overlap). C-terminal region also weakly similar to other hypothetical proteins e.g. Q9FC38|YG11_STRCO from Streptomyces coelicolor (114 aa), FASTA scores: opt: 151, E(): 0.007, (31.65% identity in 98 aa overlap). P95087 No conserved hypotheticals P95087 P95087 P95087 NC_000962.3 Mycobrowser_v5 CDS 3436779 3438053 . + 0 Rv3074 Rv3074 Function unknown Conserved hypothetical protein Rv3074, (MTCY22D7.07c), len: 424 aa. Conserved hypothetical protein, highly similar but shorter (46 aa) to P71806|Rv1378c|MTCY02B12.12c hypothetical 51.3 KDA protein from Mycobacterium tuberculosis (475 aa), FASTA scores: opt: 2009, E(): 5.8e-113, (72.95% identity in 429 aa overlap); and also similar to other hypothetical mycobacterium proteins e.g. O33266|Rv0336|MTCY279.03 (503 aa), FASTA scores: opt: 337, E(): 7.5e-13, (28.6% identity in 381 aa overlap); O33360|Rv0515|MTCY20G10.05 (503 aa), FASTA scores: opt: 337, E(): 7.5e-13, (28.6% identity in 381 aa overlap); etc. I6XG38 No conserved hypotheticals I6XG38 NC_000962.3 Mycobrowser_v5 CDS 3467606 3467926 . + 0 Rv3098A Rv3098A Possible mRNA interferase PemK-like protein Rv3098A, len: 106 aa. PemK-like protein. V5QRX7 No virulence, detoxification, adaptation V5QRX7 GO:0003677 V5QRX7 NC_000962.3 Mycobrowser_v5 CDS 3439072 3439548 . + 0 Rv3076 Rv3076 Function unknown Conserved hypothetical protein Rv3076, (MTCY22D7.05c), len: 158 aa. Conserved hypothetical protein, weakly similar to Q9AK12|SC8D11.07 hypothetical 17.0 KDA protein from Streptomyces coelicolor (151 aa), FASTA scores: opt: 110, E(): 1.5, (25.5% identity in 145 aa overlap). I6X666 No conserved hypotheticals P95082 I6X666 I6X666 NC_000962.3 Mycobrowser_v5 CDS 3441353 3441754 . + 0 Rv3078 hab Enzyme involved in the second step of nitrobenzene degradation: rearranged the intermediate hydroxylaminobenzene to 2-aminophenol. Probable hydroxylaminobenzene mutase Hab Rv3078, (MTCY22D7.03c), len: 133 aa. Probable hab, hydroxylaminobenzene mutase (5.-.-.-) (see Davis et al., 2000), highly similar to two hydroxylaminobenzene mutases from Pseudomonas pseudoalcaligenes O52214|HABA (135 aa), FASTA scores: opt: 495, E(): 6.8e-25, (51.1% identity in 133 aa overlap); and O52216|HABB (164 aa), FASTA scores: opt: 479, E(): 8.2e-24, (51.9% identity in 133 aa overlap) (see Davis et al., 2000); and to Q9AH35|NBZB hydroxylaminobenzene mutase from Pseudomonas putida (164 aa), FASTA scores: opt: 476, E(): 1.3e-23, (51.8% identity in 133 aa overlap) (see Park & Kim 2000). Gene name according to Pseudomonas pseudoalcaligenes nomenclature. Also similarity with putative different membrane proteins involved in transport (protein predicted to be a transmembrane protein). I6Y2H3 No intermediary metabolism and respiration I6Y2H3 GO:0016853 5.-.-.- NC_000962.3 Mycobrowser_v5 CDS 3447404 3448426 . - 0 Rv3082c virS May have a role in the regulation of proteins necessary for virulence. Virulence-regulating transcriptional regulator VirS (AraC/XylS family) Rv3082c, (MT3167, MTV013.03c), len: 340 aa. VirS, transcriptional regulatory protein araC/xylS family, probably involved in virulence (see citations below). Similar to many transcriptional regulators araC/xylS family e.g. Q9HZ25|PA3215 probable transcriptional regulator (AraC/XylS family) from Pseudomonas aeruginosa (337 aa), FASTA scores: opt: 379, E(): 3e-17, (30.4% identity in 306 aa overlap); Q9Z3Y6|PHBR polyhydroxybutyrate transcriptional activator from Pseudomonas sp. 61-3 (379 aa), FASTA scores: opt: 336, E(): 2e-14, (26.35% identity in 334 aa overlap); P72171|ORUR|PA0831 ornithine utilization transcriptional regulator oruR from Pseudomonas aeruginosa (339 aa), FASTA scores: opt: 274, E(): 1.9e-10, (23.7% identity in 321 aa overlap); Q9ZFW7 virulence regulating homolog from Pseudomonas alcaligenes (346 aa), FASTA scores: opt: 262, E(): 1.2e-09, (24.5% identity in 339 aa overlap); etc. Also similar to O69703|Rv3736|MTV025.084 putative regulatory protein (AraC/XylS family) from Mycobacterium tuberculosis strain H37Rv (353 aa), FASTA scores: opt: 656, E(): 3.5e-35, (36.95% identity in 333 aa overlap). Has potential helix-turn-helix motif at positions 252-273. Belongs to the AraC/XylS family of transcriptional regulators. Substrate of PknK. P9WMJ3 No virulence, detoxification, adaptation Q06861 P9WMJ3 GO:0009405,GO:0006355,GO:0043565,GO:0006350,GO:0003700,GO:0005622 P9WMJ3 NC_000962.3 Mycobrowser_v5 CDS 3446040 3447278 . + 0 Rv3081 Rv3081 Function unknown Conserved hypothetical protein Rv3081, (MTV013.02), len: 412 aa. Conserved hypothetical protein. Second part of the protein (approximately residues 250-412) shares weak similarity with other hypothetical proteins e.g. Q9YEU3|APE0488 from Aeropyrum pernix (188 aa), FASTA scores: opt: 149, E(): 0.019, E(): 0.019, (29.5% identity in 173 aa overlap); and first part shares weak similarity with C-terminal part of Q9RVT9|DR0933 alpha-amlyase from Deinococcus radiodurans (644 aa), FASTA scores: opt: 127, E(): 1.4, (27.25% identity in 198 aa overlap). Equivalent to AAK47502|MT3166 hypothetical 48.3 KDA protein from Mycobacterium tuberculosis strain CDC1551 (436 aa) but shorter 24 aa in N-terminus. Contains PS00850 Glycine radical signature and possible helix-turn-helix motif at aa 53-74. O53298 No conserved hypotheticals O53298 O53298 NC_000962.3 Mycobrowser_v5 CDS 3448504 3449991 . + 0 Rv3083 Rv3083 Function unknown; possible oxidoreductase involved in cellular metabolism. Probable monooxygenase (hydroxylase) Rv3083, (MTV013.04), len: 495 aa. Probable monooxygenase, highly similar to other putative monooxygenases flavin-binding family e.g. AAK48336|MT3969 from Mycobacterium tuberculosis strain CDC1551 (489 aa), FASTA scores: opt: 1692, E(): 4.9e-98, (49.7% identity in 489 aa overlap); Q9A588|CC2569 from Caulobacter crescentus (498 aa), FASTA scores: opt: 1684, E(): 1.6e-97, (52.25% identity in 484 aa overlap); Q9APW3 from Pseudomonas aeruginosa (508 aa), FASTA scores: opt: 1603, E(): 1.8e-92, (49.8% identity in 484 aa overlap); etc. P9WNF7 No intermediary metabolism and respiration O53300 P9WNF7 GO:0050661,GO:0004499,GO:0055114,GO:0050660 1.-.-.- P9WNF7 NC_000962.3 Mycobrowser_v5 CDS 3449997 3450923 . + 0 Rv3084 lipR Function unknown; lipolytic enzyme involved in cellular metabolism. Probable acetyl-hydrolase/esterase LipR Rv3084, (MTV013.05), len: 308 aa. Probable lipR, N-Acetyl-hydrolase/esterase, similar to other e.g. Q01109|BAH_STRH from Streptomyces hygroscopicus (299 aa), FASTA scores: opt: 558, E(): 4.1e-26, (40.25% identity in 246 aa overlap); Q9X8J4|SCE9.22 from Streptomyces coelicolor (266 aa), FASTA scores: opt: 544, E(): 2.5e-25, (36.95% identity in 257 aa overlap); Q56171|DEA from Streptomyces viridochromogenes (299 aa), FASTA scores: opt: 532, E(): 1.4e-24, (38.6% identity in 254 aa overlap); etc. Also similar to O06350|LIPF|Rv3487c|MTCY13E12.41c (277 aa), FASTA score: opt: 291, E(): 8.5e-10, (28.5% identity in 239 aa overlap). May belong to the 'GDXG' family of lipolytic enzymes. P9WK85 No intermediary metabolism and respiration O53301 P9WK85 GO:0016787 3.1.1.- P9WK85 NC_000962.3 Mycobrowser_v5 CDS 3452925 3454343 . + 0 Rv3087 Rv3087 May be involved in synthesis of triacylglycerol Possible triacylglycerol synthase (diacylglycerol acyltransferase) Rv3087, (MTV013.08), len: 472 aa. Possible triacylglycerol synthase (See Daniel et al., 2004), similar to several Mycobacterium tuberculosis proteins e.g. MTCY08D5.16, MTCY28.26, MTCY493.29c. Also similar to Q9X7A8|MLCB1610.05|ML1244 conserved membrane protein from Mycobacterium leprae (491 aa). P9WKB1 No lipid metabolism O53304 P9WKB1 GO:0006071,GO:0008610,GO:0004144 2.3.1.20 P9WKB1 NC_000962.3 Mycobrowser_v5 CDS 3451781 3452887 . + 0 Rv3086 adhD Function unknown; generates an aldehyde (or perhaps a ketone) from an alcohol. Probable zinc-type alcohol dehydrogenase AdhD (aldehyde reductase) Rv3086, (MTV013.07), len: 368 aa. Probable adhD, zinc-type alcohol dehydrogenase, highly similar to many e.g. O69045 hypothetical alcohol dehydrogenase from Rhodococcus rhodochrous (370 aa), FASTA scores: opt: 1255, E(): 8.7e-68, (50.4% identity in 367 aa overlap); P25406|ADHB_UROHA alcohol dehydrogenase I-B from Uromastyx hardwickii (Indian spiny-tailed lizard) (375 aa), FASTA scores: opt: 787, E(): 8.2e-40, (35.9% identity in 373 aa overlap); P72324||ADHI_RHOSH alcohol dehydrogenase class III from Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides) (376 aa), FASTA scores: opt: 787, E(): 8.3e-40, (35.1% identity in 379 aa overlap). Also highly similar to P71818|Rv0761c|MTCY369.06c hypothetical zinc-type alcohol dehydrogenase-like protein from Mycobacterium tuberculosis strain H37Rv (375 aa), FASTA scores: opt: 1186, E(): 1.2e-63, (47.3% identity in 368 aa overlap). Contains PS00059 Zinc-containing alcohol dehydrogenases signature. Belongs to the zinc-containing alcohol dehydrogenase. Possibly requires zinc for its activity. P9WQB9 No intermediary metabolism and respiration O53303 P9WQB9 GO:0016491,GO:0008270,GO:0055114 1.1.1.- P9WQB9 NC_000962.3 Mycobrowser_v5 CDS 3454340 3455764 . + 0 Rv3088 tgs4 May be involved in synthesis of triacylglycerol Putative triacylglycerol synthase (diacylglycerol acyltransferase) Tgs4 Rv3088, (MTV013.09), len: 474 aa. Putative tgs4, triacylglycerol synthase (See Daniel et al., 2004), similar to several Mycobacterium tuberculosis proteins e.g. MTCY31.23 (505 aa), MTCY13E12.34c (497 aa) and MTCY493.29c (459 aa). Also similar to Q9X7A8|MLCB1610.05|ML1244 conserved membrane protein from Mycobacterium leprae (491 aa). P9WKC3 No lipid metabolism P67208 P9WKC3 GO:0006071,GO:0008610,GO:0004144 2.3.1.20 NC_000962.3 Mycobrowser_v5 CDS 3458211 3459098 . + 0 Rv3090 Rv3090 Unknown Unknown alanine and valine rich protein Rv3090, (MTCY164.01), len: 295 aa. Unknown Ala-, Val-rich protein. Hydrophobic stretch at N-terminus. O05769 No conserved hypotheticals O05769 O05769 O05769 NC_000962.3 Mycobrowser_v5 CDS 3480074 3480742 . + 0 Rv3113 Rv3113 Function unknown Possible phosphatase Rv3113, (MTCY164.23), len: 222 aa. Possible phosphatase, with weak similarity to other phosphatases e.g. Q9KYY0|SCE33.02c from Streptomyces coelicolor (223 aa), FASTA scores: opt: 368, E(): 1.2e-16, (32.9% identity in 222 aa overlap); and Q55039|GPH_SYNP7|CBBZ phosphoglycolate phosphatase from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (212 aa), FASTA scores: opt: 176, E(): 0.00025, (24.7% identity in 182 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O05790 No intermediary metabolism and respiration O05790 O05790 GO:0008967,GO:0008152 3.1.3.- O05790 NC_000962.3 Mycobrowser_v5 CDS 3461760 3462764 . - 0 Rv3093c Rv3093c Function unknown; could be involved in cellular metabolism. Hypothetical oxidoreductase Rv3093c, (MTCY164.04c), len: 334 aa. Hypothetical oxidoreductase, with some similarity with various oxidoreductases e.g. Q58929|mer|MJ1534 N5,N10-methylene tetrahydromethanopterin reductase from Methanococcus jannaschii (331 aa), FASTA scores: opt: 300, E(): 1.1e-10, (24.1% identity in 324 aa overlap); and Q9ZA30|GRA-ORF29 putative FMN-dependent monooxygenase from Streptomyces violaceoruber (343 aa), FASTA scores: opt: 264, E(): 1.5e-08, (30.45% identity in 335 aa overlap); Q9CCV8|ML0348 possible coenzyme F420-dependent oxidoreductase from Mycobacterium leprae (350 aa), FASTA scores: opt: 220, E(): 6.4e-06, (26.5% identity in 328 aa overlap); etc. O05772 No intermediary metabolism and respiration O05772 O05772 GO:0016491,GO:0055114 1.-.-.- O05772 NC_000962.3 Mycobrowser_v5 CDS 3462761 3463891 . - 0 Rv3094c Rv3094c Function unknown Conserved hypothetical protein Rv3094c, (MTCY164.05c), len: 376 aa. Conserved hypothetical protein, some similarity with various proteins e.g. Q9RMR9|NRGC NRGC protein (corresponding gene seems regulated by NifA) from Bradyrhizobium japonicum (388 aa), FASTA scores: opt: 677, E(): 5.8e-35, (34.55% identity in 353 aa overlap); P26698|PIGM_RHOSO pigment protein from Rhodococcus sp. strain ATCC 21145 (387 aa), FASTA scores: opt: 480, E(): 1.2e-22, (28.7% identity in 376 aa overlap); Q9F0J3|NCNH hydroxylase from Streptomyces arenae (405 aa), FASTA scores: opt: 441, E(): 3.3e-20, (29.25% identity in 352 aa overlap); etc. Equivalent to AAK47516 from Mycobacterium tuberculosis strain CDC1551 (395 aa) but N-terminus shorter 19 aa. O05773 No conserved hypotheticals O05773 O05773 GO:0003995,GO:0055114,GO:0050660 O05773 NC_000962.3 Mycobrowser_v5 CDS 3463973 3464449 . + 0 Rv3095 Rv3095 Unknown. Could be involved in regulatory mechanism. Hypothetical transcriptional regulatory protein Rv3095, (MTCY164.06), len: 158 aa. Possible regulatory protein, because contains possible helix-turn-helix motif at aa 39-61 (+4.83 SD). Similar to hypothetical proteins e.g. Q9I0C9|PA2713 from Pseudomonas aeruginosa (159 aa), FASTA scores: opt: 486, E(): 1.6e-25, (45.95% identity in 148 aa overlap); Q9AAF6|CC0645 from Caulobacter crescentus (188 aa), FASTA scores: opt: 479, E(): 5.3e-25, (45.75% identity in 153 aa overlap); Q9K408|2SCG61.07 from Streptomyces coelicolor (157 aa), FASTA scores: opt: 407, E(): 2.8e-20, (43.9% identity in 139 aa overlap); etc. P9WMG3 No regulatory proteins P0A648 P9WMG3 GO:0045449,GO:0006350,GO:0003677 P9WMG3 NC_000962.3 Mycobrowser_v5 CDS 3464547 3465686 . + 0 Rv3096 Rv3096 Function unknown Conserved hypothetical protein Rv3096, (MTCY164.07), len: 379 aa. Hypothetical protein, with slight similarity to several proteins e.g. Q09671|OYEB_SCHPO|SPAC5H10.10 putative NADPH dehydrogenase C5H10.10 (old yellow enzyme homolog) from Schizosaccharomyces pombe (Fission yeast) (392 aa), FASTA scores: opt: 125, E(): 1.1, (25.45% identity in 165 aa overlap); and Q12603|XYNA_DICTH beta-1,4-xylanase (endo-1,4-beta-xylanase) from Dictyoglomus thermophilum (352 aa), FASTA scores: opt: 124, E(): 1.2, (25.65% identity in 195 aa overlap); etc. Contains glycosyl hydrolases family 5 signature (PS00659). Predicted to be an outer membrane protein (See Song et al., 2008). I6YB54 No conserved hypotheticals I6YB54 GO:0003824,GO:0043169,GO:0005975 I6YB54 NC_000962.3 Mycobrowser_v5 CDS 3467210 3467662 . - 0 Rv3098c Rv3098c Unknown Hypothetical protein Rv3098c, (MTCY164.09c), len: 150 aa. Hypothetical unknown protein (shorter version of MTCY164.09c). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O05776 No conserved hypotheticals O05776 NC_000962.3 Mycobrowser_v5 CDS 3470680 3471369 . - 0 Rv3102c ftsE Involved in growth. Thought to be involved in active transport of septation component across the membrane. Responsible for energy coupling to the transport system. Is coded in an operon essential for cell division. Putative cell division ATP-binding protein FtsE (septation component-transport ATP-binding protein ABC transporter) Rv3102c, (MTCY164.13_2c), len: 229 aa. Putative ftsE, cell division protein, septation component transport ATP-binding protein ABC transporter (see citations below), equivalent to O32883|FTSE|ML0669 cell division ATP-binding protein from Mycobacterium leprae (229 aa), FASTA scores: opt: 1384, E(): 2.4e-74, (91.7% identity in 229 aa overlap); and similar to Q9L1S6|FTSE from Streptomyces coelicolor (229 aa), FASTA scores: opt: 914, E(): 8.7e-47, (62.85% identity in 226 aa overlap); Q9A0S4|FTSE|SPY0644 from Streptococcus pyogenes (230 aa), FASTA scores: opt: 866, E(): 5.7e-44, (57.9% identity in 228 aa overlap); Q9CGX0|FTSE from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (230 aa), FASTA scores: opt: 792, E(): 1.3e-39, (52.2% identity in 228 aa overlap); etc. Other relatives from Mycobacterium tuberculosis include: MTCY253.24; MTCY16B7.10; MTCY9C4.04c; MTCY50.01; MTCY05A6.09c; MTCY04C12.31. Contains PS00017 ATP/GTP-binding site motif A (P-loop) and ABC transporters family signature (PS00211). Belongs to the ATP-binding transport protein family (ABC transporters). O05779 No cell wall and cell processes O05779 O05779 GO:0016887,GO:0051301,GO:0005524 O05779 NC_000962.3 Mycobrowser_v5 CDS 3471413 3471850 . - 0 Rv3103c Rv3103c Unknown Hypothetical proline-rich protein Rv3103c, (MTCY164.13c), len: 145 aa. Hypothetical unknown pro-rich protein, with some similarity to Proline-rich proteins e.g. Q39789 proline-rich cell wall protein from Gossypium hirsutum (Upland cotton) (214 aa), FASTA scores: opt: 267, E(): 0.00014, (40% identity in 110 aa overlap). Equivalent to AAK47525 from M. tuberculosis strain CDC1551 (158 aa) but shorter 13 aa. O05780 No conserved hypotheticals O05780 NC_000962.3 Mycobrowser_v5 CDS 3471852 3472778 . - 0 Rv3104c Rv3104c Unknown Possible conserved transmembrane protein Rv3104c, (MTCY164.14c), len: 308 aa. Possible conserved transmembrane protein, with some similarity to hypthetical proteins e.g. Q9L1X9|SC8E4A.26 putative membrane protein from Streptomyces coelicolor (408 aa), FASTA scores: opt: 514, E(): 4.3e-25, (35.2% identity in 287 aa overlap); Q9XA89|CF43A.26c hypothetical 36.1 KDA protein from Streptomyces coelicolor (333 aa), FASTA scores: opt: 482, E(): 3.7e-23, (34.9% identity in 301 aa overlap); Q55987|SLR0765 hypothetical 68.9 KDA protein from Synechocystis sp. strain PCC 6803 (617 aa), FASTA scores: opt: 429, E(): 1.3e-19, (30.6% identity in 278 aa overlap); etc. O05781 No cell wall and cell processes O05781 O05781 GO:0055085,GO:0016021 O05781 NC_000962.3 Mycobrowser_v5 CDS 3811022 3811636 . - 0 Rv3395c Rv3395c Function unknown Conserved hypothetical protein Rv3395c, (MTCY78.33), len: 204 aa. Conserved hypothetical protein, with some similarity with RECA proteins (recombinases A) e.g. P16238|RECA_THIFE from Thiobacillus ferrooxidans (346 aa), FASTA scores: opt: 131, E(): 1.1, (31.45% identity in 140 aa overlap); Q59560|RECA_MYCSM from Mycobacterium smegmatis (349 aa), FASTA scores: opt: 121, E(): 4.4, (30.25% identity in 129 aa overlap); etc. Note that shortened since first submission to avoid overlap with Rv3395A. Equivalent to AAK47839 from Mycobacterium tuberculosis strain CDC1551 (227 aa) but shorter 23 aa. P9WKZ9 No conserved hypotheticals P9WKZ9 GO:0016021 P9WKZ9 NC_000962.3 Mycobrowser_v5 CDS 3474007 3475377 . + 0 Rv3106 fprA Generates oxidized ferredoxin from ferredoxin [catalytic activity: reduced ferredoxin + NADP(+) = oxidized ferredoxin + NADPH]. NADPH:adrenodoxin oxidoreductase FprA (NADPH-ferredoxin reductase) Rv3106, (MTCY164.16), len: 456 aa. FprA, NADPH:adrenodoxin oxidoreductase (NADPH-ferredoxin reductase) (see citations below), equivalent to O32886|MLCB1779.25|FPRA|ML0666 from Mycobacterium leprae (456 aa), FASTA scores: opt: 2505, E(): 1.2e-142, (81,05% identity in 459 aa overlap); also similar to other NADPH:adrenodoxin oxidoreductases e.g. Q9RX19|DR0496 from Deinococcus radiodurans (479 aa), FASTA scores: opt: 1331, E(): 2.6e-72, (48.9% identity in 454 aa overlap); Q9RK35|SCF15.02 from Streptomyces coelicolor (454 aa), FASTA scores: opt: 1102, E(): 1.3e-58, (41.35% identity in 462 aa overlap); P82861 from Salvelinus fontinalis (Brook trout) (498 aa), FASTA scores: opt: 827, E(): 4e-42, (41.3% identity in 460 aa overlap); Q9V3T9|ADRO_DROME from Drosophila melanogaster (Fruit fly) (466 aa), FASTA scores: opt: 790, E(): 6.3e-40, (39.45% identity in 459 aa overlap); etc. Also similar to Q10547|FPRB|Rv0886|MT0909|MTCY31.14 from Mycobacterium tuberculosis strain H37Rv (575 aa), FASTA scores: opt: 894, E(): 4.4e-46, (42.05% identity in 459 aa overlap). Cofactor: FAD P9WIQ3 No intermediary metabolism and respiration 1LQT,1LQU,2C7G O05783 P9WIQ3 GO:0055114,GO:0004324 1.18.1.2 P9WIQ3 NC_000962.3 Mycobrowser_v5 CDS 3479171 3479683 . + 0 Rv3111 moaC1 Involved in the biosynthesis of molybdopterin. Probable molybdenum cofactor biosynthesis protein C MoaC1 Rv3111, (MTCY164.21), len: 170 aa. Probable moaC1, molybdopterin cofactor biosynthesis protein, highly similar to others e.g. Q9HX95|MOAC|PA3918 from Pseudomonas aeruginosa (160 aa), FASTA scores: opt: 576, E(): 2.2e-29, (62.1% identity in 153 aa overlap); Q9ZFA6|MOAC from Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides) (159 aa), FASTA scores: opt: 541, E(): 3.4e-27, (59.85% identity in 157 aa overlap); BAB48171|MLR0616 from Rhizobium loti (Mesorhizobium loti) (160 aa), FASTA scores: opt: 531, E(): 1.5e-26, (58.75% identity in 160 aa overlap); P30747|MOAC_ECOLI|CHLA3|B0783 from Escherichia coli strain K12 (160 aa), FASTA scores: opt: 527, E(): 2.6e-26, (58.5% identity in 159 aa overlap); etc. Also highly similar to O53376|MOAC3|Rv3324c|MTV016.24c putative molybdenum cofactor biosynthesis protein C 3 from Mycobacterium tuberculosis (177 aa), FASTA scores: opt: 738, E(): 1.7e-39, (71.5% identity in 165 aa overlap); AAK47767|MT3425 molybdopterin cofactor biosynthesis protein C from Mycobacterium tuberculosis strain CDC1551 (184 aa), FASTA scores: opt: 734, E(): 3.1e-39, (71.8% identity in 163 aa overlap); and Rv0864|MOAC2|MTV043.57 putative molybdenum cofactor biosynthesis protein C 2 (167 aa). Note that previously known as moaC. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WJR9 No intermediary metabolism and respiration P0A5K4 P9WJR9 GO:0006777 P9WJR9 NC_000962.3 Mycobrowser_v5 CDS 3485572 3486174 . + 0 Rv3120 Rv3120 Function unknown Conserved hypothetical protein Rv3120, (MTCY164.30), len: 200 aa. Conserved hypothetical protein, with weak similarity to several hypothetical proteins and many N-methyl transferases e.g. Q9X9V1|ORF8 putative methyltransferase from Streptomyces coelicolor A3(2) (208 aa), FASTA scores: opt: 177, E(): 0.00011, (34.6% identity in 130 aa overlap); Q9XA90|SCF43A.25c putative methyltransferase from Streptomyces coelicolor (215 aa), FASTA scores: opt: 147, E(): 0.011, (31.3% identity in 166 aa overlap); BAB52127|MLL5735 probable methyltransferase from Rhizobium loti (Mesorhizobium loti) (247 aa), FASTA scores: opt: 133, E(): 0.11, (29.75% identity in 158 aa overlap). Highly similar to O53374|Rv3322c|MTV016.22c possible methyltransferase from Mycobacterium tuberculosis strain H37Rv (204 aa), FASTA scores: opt: 691, E(): 1.1e-38, (57.0% identity in 200 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O05796 No conserved hypotheticals O05796 O05796 GO:0008168,GO:0008152 O05796 NC_000962.3 Mycobrowser_v5 CDS 3510088 3511317 . - 0 Rv3144c PPE52 Function unknown PPE family protein PPE52 Rv3144c, (MTCY03A2.14), len: 409 aa. PPE52, Member of the Mycobacterium tuberculosis PPE family, Gly-, Ala-rich, similar to others e.g. P71868|Rv3533c|MTCY03C7.23 (582 aa), FASTA scores: opt: 1007, E(): 5.2e-35, (56.2% identity in 306 aa overlap); and MTV014_3, MTCY6G11_5, MTCY98.0034c, MTCY31.06c, MTCY48.17, MTCY98.0029c, MTCY03C7.17c, etc. Nucleotide position 3510642 in the genome sequence has been corrected, T:C resulting in S226G. I6X6H8 No PE/PPE Q6MX05 I6X6H8 I6X6H8 NC_000962.3 Mycobrowser_v5 CDS 3477060 3477500 . + 0 Rv3108 Rv3108 Unknown Hypothetical protein Rv3108, (MTCY164.18), len: 146 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O05785 No conserved hypotheticals O05785 NC_000962.3 Mycobrowser_v5 CDS 3478779 3479174 . + 0 Rv3110 moaB1 Thought to be involved in molybdopterin biosynthesis. Catalyzes the dehydratation of 4A-hydroxytetrahydropterins [catalytic activity: (6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4A-hydroxypterin = (6R)-6-(L-erythro-1,2- dihydroxypropyl)-7,8-dihydro-6H-pterin + H(2)O]. Probable pterin-4-alpha-carbinolamine dehydratase MoaB1 (PHS) (4-alpha- hydroxy-tetrahydropterin dehydratase) (pterin-4-a-carbinolamine dehydratase) (phenylalanine hydroxylase-stimulating protein) (PHS) (pterin carbinolamine dehydratase) (PCD) Rv3110, (MTCY164.20), len: 131 aa. Probable moaB1, pterin-4-alpha-carbinolamine dehydratase, similar to others e.g. P73790|SSL2296 from Synechocystis sp. strain PCC 6803 (96 aa), FASTA scores: opt: 195, E(): 6.2e-07, (35.4% identity in 96 aa overlap); Q9PAB4|PHS_XYLFA|XF2604 from Xylella fastidiosa (116 aa), FASTA scores: opt: 187, E(): 2.6e-06, (36.25% identity in 102 aa overlap); AAK42360|Q97WM6|PHS_SULSO|SSO2187 from Sulfolobus solfataricus (114 aa), FASTA scores: opt: 177, E(): 1.3e-05, (34.6% identity in 78 aa overlap); etc. Also highly similar to AAK47768|MT3426 pterin-4-alpha-carbinolamine dehydratase from Mycobacterium tuberculosis CDC1551 (124 aa), FASTA scores: opt: 383, E(): 7.7e-20, (50.0% identity in 110 aa overlap). Belongs to the pterin-4-alpha-carbinolamine dehydratase family. Note that previously known as moaB. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q6MX13 No intermediary metabolism and respiration Q6MX13 Q6MX13 GO:0006729,GO:0008124 Q6MX13 NC_000962.3 Mycobrowser_v5 CDS 3479700 3479951 . + 0 Rv3112 moaD1 Involved in molybdenum cofactor biosynthesis. Probable molybdenum cofactor biosynthesis protein D MoaD1 (molybdopterin converting factor small subunit) (molybdopterin [MPT] converting factor, subunit 1) Rv3112, (MTCY164.22), len: 83 aa. Probable moaD1, molybdenum cofactor biosynthesis protein (molybdopterin converting factor (subunit 1)), similar to others e.g. Q9HJF0|TA1019 from Thermoplasma acidophilum (85 aa), FASTA scores: opt: 144, E(): 0.0012, (31.7% identity in 82 aa overlap); BAB59710|TVG0556526 from Thermoplasma volcanium (90 aa), FASTA scores: opt: 144, E(): 0.0012, (31.7% identity in 82 aa overlap); P30748|MOAD_ECOLI|CHLA4|CHLM|B0784 from Escherichia coli strain K12 (81 aa), FASTA scores: opt: 116, E(): 0.11, (36.9% identity in 84 aa overlap); etc. N-terminus also highly similar to to O53375|GPHA|Rv3323c|MTV016.23c MOAD-MOAE fusion protein from Mycobacterium tuberculosis (221 aa), FASTA scores: opt: 333, E(): 2e-16, (65.05% identity in 83 aa overlap); and some similarity with Rv0868c|MTV043.61c|MOAD2 putative molybdenum cofactor biosynthesis protein D 2 (92 aa). Note that previously known as moaD. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L7N6B4 No intermediary metabolism and respiration Q7D640 L7N6B4 GO:0006790,GO:0006777 L7N6B4 NC_000962.3 Mycobrowser_v5 CDS 3483974 3484807 . + 0 Rv3117 cysA3 May be a sulfotransferase involved in the formation of thiosulfate [catalytic activity: thiosulfate + cyanide = sulfite + thiocyanate]. Probable thiosulfate sulfurtransferase CysA3 (rhodanese-like protein) (thiosulfate cyanide transsulfurase) (thiosulfate thiotransferase) Rv3117, (MTCY164.27, MT3199, O05793), len: 277 aa. Probable cysA3 (alternate gene name: sseC3), thiosulfate sulfurtransferase (see Wooff et al., 2002), equivalent to Q50036|CYSA|CYSA3|ML2198|THTR_MYCLE putative sulfurtransferase thiosulfate from Mycobacterium leprae (277 aa). Also highly similar to other putative thiosulfate sulfurtransferases e.g. P16385|THTR_SACER|CYSA from Saccharopolyspora erythraea (Streptomyces erythraeus) (281 aa), FASTA scores: opt: 1442, E(): 1.7e-84, (75.55% identity in 274 aa overlap); Q9RXT9DR0217|DR0217 from Deinococcus radiodurans (286 aa), FASTA scores: opt: 1046, E(): 2.6e-59, (53.8% identity in 275 aa overlap); Q9HMT7|TSSA|VNG2393G from Halobacterium sp. strain NRC-1 (293 aa), FASTA scores: opt: 1030, E(): 2.7e-58, (56.1% identity in 278 aa overlap); Q9Y8N8|APE2595 from Aeropyrum pernix (218 aa), FASTA scores: opt: 808, E(): 2.7e-44, (53.5% identity in 215 aa overlap); etc. Identical second copy present as Rv0815c|AL022004|MTV043.07c|MT0837|O05793|cysA2 (277 aa) (100.0% identity in 277 aa overlap). Also shows some similarity to P96888|THT2_MYCTU|SSEA|Rv3283|MT3382|MTCY71.23 putative thiosulfate sulfurtransferase from Mycobacterium tuberculosis (297 aa), FASTA scores: opt: 955, E(): 1.6e-53, (50.2% identity in 271 aa overlap); and Q59570|THT3_MYCTU|SSEB|Rv2291|MT2348|MTCY339.19c putative thiosulfate sulfurtransferase from Mycobacterium tuberculosis (284 aa), FASTA scores: E(): 1.4e-14, (26.7% identity in 292 aa overlap). Contains rhodanese active site and C-terminal signatures (PS00380, PS00683). Belongs to the rhodanese family. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WHF9 No intermediary metabolism and respiration 3AAX,3AAY P9WHF9 GO:0004792,GO:0008272 2.8.1.1 P9WHF9 NC_000962.3 Mycobrowser_v5 CDS 3492147 3493181 . + 0 Rv3127 Rv3127 Function unknown Conserved protein Rv3127, (MTCY164.37), len: 344 aa. Conserved protein, highly similar to Mycobacterium tuberculosis protein O53476|Rv2032|MTV018.19 (331 aa), FASTA scores: opt: 1212, E(): 6e-69, (56.7% identity in 321 aa overlap), and also similar to P95195|MTCY03A2.27c (332 aa), FASTA scores: opt: 521, E(): 1.6e-25; (35.0% identity in 326 aa overlap). Some similarity to C-terminal half of hypothetical Mycobacterium tuberculosis proteins. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WL07 No conserved hypotheticals P9WL07 P9WL07 NC_000962.3 Mycobrowser_v5 CDS 3531208 3531645 . - 0 Rv3162c Rv3162c Unknown Possible integral membrane protein Rv3162c, (MTV014.06c), len: 145 aa. Possible integral membrane protein, with some similarity to C-terminal part of Q10803|Rv2877c|MTCY274.08c hypothetical protein from Mycobacterium tuberculosis (287 aa), FASTA scores: opt: 112, E(): 6.9, (29.65% identity in 135 aa overlap); and other hypothetical proteins from other organisms. O53312 No cell wall and cell processes O53312 NC_000962.3 Mycobrowser_v5 CDS 3493168 3494181 . - 0 Rv3128c Rv3128c Function unknown Conserved hypothetical protein Rv3128c, (MTCY164.38c), len: 337 aa. Conserved hypothetical protein, similar to other conserved hypothetical proteins. This ORF corresponds to a fusion of MTCY164.38 and MTCY164.39c. Has in-frame amber stop codon but is similar throughout its length to Rv2807|MTCY16B7.36c|Z81331 conserved hypothetical protein from Mycobacterium tuberculosis (384 aa), FASTA scores: opt: 954, E(): 0, (47.2% identity in 339 aa overlap). Yes conserved hypotheticals NC_000962.3 Mycobrowser_v5 CDS 3486509 3487711 . + 0 Rv3121 cyp141 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Probable cytochrome P450 141 Cyp141 Rv3121, (MTCY164.31), len: 400 aa. Probable cyp141, cytochrome P-450 integral membrane protein, similar to other cytochrome P450-dependent oxidases e.g. Q9X5P9|CYP107N1 from Streptomyces lavendulae (410 aa), FASTA scores: opt: 825, E(): 3.1e-42, (33.35% identity in 393 aa overlap); Q59819|OLEP|CYP107D1 from Streptomyces antibioticus (407 aa), FASTA scores: opt: 812, E(): 1.9e-41, (34.85% identity in 396 aa overlap); O32460|CYP107M1 from Actinomadura hibisca (411 aa), FASTA scores: opt: 713, E(): 1.6e-35, (31.05% identity in 396 aa overlap); P55544|CPXP_RHISN|CYP112A|Y4LD from Rhizobium sp. strain NGR234 (400 aa), FASTA scores: opt: 688, E(): 5.1e-34, (33.0% identity in 406 aa overlap); etc. Also similar to MTCY339.44c, MTCY369.22, MTCY50.26, MTCY03C7.11, MTCY339.34c, MTCY339.42, MTCY369.11c. Contains cytochrome P450 cysteine heme-iron ligand signature (PS00086). Belongs to the cytochrome P450 family. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WPL7 No intermediary metabolism and respiration O08362 P9WPL7 GO:0020037,GO:0016021,GO:0004497,GO:0055114,GO:0005886,GO:0009055 1.14.-.- P9WPL7 NC_000962.3 Mycobrowser_v5 CDS 3489506 3490375 . + 0 Rv3124 moaR1 Involved in transcriptional mechanism. Transcriptional regulatory protein MoaR1 Rv3124, (MTCY164.34), len: 289 aa. MoaR1, transcriptional regulatory protein, similar to many Streptomyces and Mycobacterium tuberculosis regulatory proteins e.g. Q11052|YC67_MYCTU|Rv1267c|MT1305|MTCY50.15 from Mycobacterium tuberculosis strain H37Rv (388 aa), FASTA scores: opt: 963, E(): 2e-56, (55.15% identity in 252 aa overlap); O53145 from Mycobacterium tuberculosis (381 aa); P71484|EMBR from Mycobacterium avium (384 aa), FASTA scores: opt: 859, E(): 1.5e-49, (52.2% identity in 249 aa overlap); Q9XCC3|TYLT from Streptomyces fradiae (404 aa), FASTA scores: opt: 462, E(): 3.1e-23, (35.05% identity in 254 aa overlap); Q9XCC4|TYLS from Streptomyces fradiae (277 aa), FASTA scores: opt: 456, E(): 5.6e-23, (33.45% identity in 269 aa overlap); etc. Start chosen by similarity, alternative possible (see AAK47548 from Mycobacterium tuberculosis strain CDC1551, longer N-terminus (311 aa)). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O05797 No regulatory proteins O05797 O05797 GO:0005622,GO:0006355,GO:0000156,GO:0000160,GO:0003677 EMB O05797 NC_000962.3 Mycobrowser_v5 CDS 3494660 3494992 . + 0 Rv3129 Rv3129 Function unknown Conserved hypothetical protein Rv3129, (MTCY164.40), len: 110 aa. Conserved hypothetical protein, with some similarity to various hypothetical proteins from Streptomyces coelicolor e.g. Q9RI34|SCJ12.26 hypothetical 14.5 KDA protein (137 aa), FASTA scores: opt: 141, E(): 0.0016, (39.3% identity in 84 aa overlap); Q9RI49|SCJ12.09c hypothetical 15.8 KDA protein (146 aa), FASTA scores: opt: 141, E(): 0.0017, (38.05% identity in 92 aa overlap); Q9RJ05|SCJ1.09C possible DNA-binding protein (233 aa), FASTA scores: opt: 140, E(): 0.0029, (34.85% identity in 89 aa overlap); Q9XA48|SCGD3.31c putative branched-chain alpha keto acid dehydrogenase E1 beta subunit (334 aa); etc. P9WL05 No conserved hypotheticals P9WL05 P9WL05 NC_000962.3 Mycobrowser_v5 CDS 3843036 3843734 . + 0 Rv3426 PPE58 Function unknown PPE family protein PPE58 Rv3426, (MTCY78.03c), len: 232 aa. PPE58, Member of the M. tuberculosis PPE family, similar to many e.g. the downstream O06246|Rv3429|MTCY77.01 (178 aa), FASTA scores: opt: 555, E(): 6.5e-26, (72.0% identity in 125 aa overlap); and upstream Q50703|YY25_MYCTU|Rv3425|MTCY78.04c (176 aa), FASTA scores: opt: 517, E(): 1.1e-23, (68.0% identity in 125 aa overlap); MTV049_30, MTCY3C7_24, MTCY428_16, MTCY3A2_22; etc. Q50702 No PE/PPE Q50702 Q50702 Q50702 NC_000962.3 Mycobrowser_v5 CDS 3494975 3496366 . - 0 Rv3130c tgs1 Involved in synthesis of triacylglycerol Triacylglycerol synthase (diacylglycerol acyltransferase) Tgs1 Rv3130c, (MTCY03A2.28, MTCY164.41c), len: 463 aa. tgs1, triacylglycerol synthase (See Daniel et al., 2004; Sirakova et al., 2006), similar to several hypothetical Mycobacterium tuberculosis strain H37Rv proteins e.g. O06795|YH60_MYCTU|Rv1760|MTCY28.26 hypothetical 54.1 KDA protein (502 aa), FASTA scores: opt: 586, E(): 9.8e-29, (28.95% identity in 463 aa overlap). Predicted possible vaccine candidate (See Zvi et al., 2008). P9WKC9 No lipid metabolism P0A650 P9WKC9 GO:0006071,GO:0008610,GO:0004144 2.3.1.20 NC_000962.3 Mycobrowser_v5 CDS 3497529 3499265 . - 0 Rv3132c devS Sensor part of the two component regulatory system DEVR/DEVS/dost. Thought to control HSPX|Rv2031|ACR expression; O2, NO and CO are ligands, DOSS is inactive in the presence of oxygen Two component sensor histidine kinase DevS Rv3132c, (MTCY03A2.26), len: 578 aa. DevS (alternate gene name: dosS), membrane-bound two component sensor histidine kinase (see citations below; dev for Differentially Expressed in Virulent strain), similar to others two component sensors e.g. Q9RI43|SCJ12.15c putative two-component sensor from Streptomyces coelicolor (585 aa), FASTA scores: opt: 1305, E(): 2.5e-69, (41.35% identity in 573 aa overlap); Q9ZBY4|SCD78.15 putative two component sensor from Streptomyces coelicolor (560 aa), FASTA scores: opt: 1194, E(): 8.1e-63, (41.05% identity in 558 aa overlap); O85371|CPRS two component regulator from Rhodococcus sp (563 aa), FASTA scores: opt: 803, E(): 8.3e-40, (38.4% identity in 552 aa overlap); Q9L094|SCC24.23 putative two-component sensor histidine kinase from Streptomyces coelicolor (similarity only in C-terminus for this one); etc. Also highly similar to mycobacterium O53473|Rv2027c|MTV018.14c putative membrane protein (573 aa), FASTA scores: opt: 2333, E(): 7.6e-130, (61.45% identity in 576 aa overlap). Predicted possible vaccine candidate (See Zvi et al., 2008). Contains GAF domain that binds heme. P9WGK3 No regulatory proteins 2W3D,2W3E,2W3F,2W3G,2W3H,3ZXO,2Y79 P95194 P9WGK3 GO:0005737,GO:0016021,GO:0046872,GO:0018106,GO:0046983,GO:0007165,GO:0000155,GO:0000160,GO:0005524 2.7.13.3 P9WGK3 NC_000962.3 Mycobrowser_v5 CDS 3811719 3812345 . + 0 Rv3395A Rv3395A Unknown Probable membrane protein Rv3395A, len: 208 aa. Probable membrane protein, with potential transmembrane stretches from aa 7..25 and 55..77. Weak similarity to Q9F2P3|SCE41.16C putative lipoprotein from Streptomyces coelicolor (258 aa), FASTA scores: opt: 107, E(): 7.4, (34.05% identity in 94 aa overlap). Q6MWX4 No cell wall and cell processes Q6MWX4 Q6MWX4 NC_000962.3 Mycobrowser_v5 CDS 3499262 3499915 . - 0 Rv3133c devR Regulator part of the two component regulatory system DEVR/DEVS/dost. Controls HSPX|Rv2031|ACR expression. Two component transcriptional regulatory protein DevR (probably LuxR/UhpA-family) Rv3133c, (MTCY03A2.25), len: 217 aa. DevR (alternate gene name: dosR), two component transcriptional regulator (see Dasgupta et al., 2000; dev for Differentially Expressed in Virulent strain), highly similar to several e.g. O85372|CPRR two component regulator from Rhodococcus sp. (212 aa), FASTA scores: opt: 868, E(): 6.2e-46, (65.05% identity in 206 aa overlap); Q9RI42|SCJ12.16c putative LuxR family two-component response regulator from Streptomyces coelicolor (233 aa), FASTA scores: opt: 849, E(): 9.7e-45, (60.55% identity in 218 aa overlap); Q9XA59|SCGD3.19 putative two-component system response transcriptional regulator from Streptomyces coelicolor (218 aa), FASTA scores: opt: 835, E(): 6.5e-44, (61.55% identity in 208 aa overlap); and similar to others. Contains bacterial regulatory proteins, LuxR family signature (PS00622) near C-terminus as seen in bvgA, comA, dctR, degU, evgA, fimZ, fixJ, gacA, glpR, narL, narP, nodW, rcsB and uhpA. Helix-turn-helix motif at 166-187 (+3.15 SD). Belongs to the LuxR/UhpA family of transcriptional regulators. The N-terminal region is similar to that of other regulatory components of sensory transduction systems. P9WMF9 No regulatory proteins 1ZLJ,1ZLK,3C3W,3C57 P95193 P9WMF9 GO:0044161,GO:0006355,GO:0043565,GO:0006350,GO:0003700,GO:0000156,GO:0000160,GO:0005737 P9WMF9 NC_000962.3 Mycobrowser_v5 CDS 3503393 3504175 . + 0 Rv3137 Rv3137 Function unknown; involved in cellular metabolism. Probable monophosphatase Rv3137, (MTCY03A2.21c), len: 260 aa. Probable monophosphatase, equivalent to O32889|MLCB1779_19|ML0662 putative monophosphatase from Mycobacterium leprae (255 aa), FASTA scores: opt: 1403, E(): 1.2e-81, (81.8% identity in 253 aa overlap). Also similar to Q9K4B1|SC7E4.05c from Streptomyces coelicolor (266 aa), FASTA scores: opt: 969, E(): 3.5e-54, (57.9% identity in 259 aa overlap); Q53743|PUR3 mono-phosphatase from Streptomyces lipmanii (Streptomyces alboniger) (273 aa), FASTA scores: opt: 862, E(): 2.1e-47, (55.25% identity in 257 aa overlap); BAB50023|MLL3039 mono-phosphatase from Rhizobium loti (Mesorhizobium loti) (262 aa), FASTA scores: opt: 448, E(): 3.2e-21, (31.37% identity in 255 aa overlap); etc. Contains inositol monophosphatase family signature 1 (PS00629). P95189 No intermediary metabolism and respiration P95189 P95189 GO:0004437 3.1.3.- P95189 NC_000962.3 Mycobrowser_v5 CDS 3504195 3505283 . + 0 Rv3138 pflA Involved in cellular metabolism [catalytic activity: S-adenosyl-L-methionine + dihydroflavodoxin + [formate acetyltransferase]-glycine = 5'-deoxyadenosine + methionine + flavodoxin + [formate acetyltransferase]-glycine-2-YL radical]. Probable pyruvate formate lyase activating protein PflA (formate acetyltransferase activating enzyme) ([pyruvate formate-lyase] activating enzyme) Rv3138, (MTCY03A2.20c), len: 362 aa. Probable pflA, pyruvate formate lyase activating protein, similar to other e.g. Q9V0N1|PAB1859 from Pyrococcus abyssi (348 aa), FASTA scores: opt: 926, E(): 1.1e-52, (39.95% identity in 343 aa overlap); O27446|MTH1395 from Methanobacterium thermoautotrophicum (335 aa), FASTA scores: opt: 909, E(): 1.3e-51, (42.2% identity in 327 aa overlap); O28939|AF1330 from Archaeoglobus fulgidus (336 aa), FASTA scores: opt: 884, E(): 5.6e-50, (42.0% identity in 319 aa overlap); etc. Also similar to O50099|PH1391 hypothetical 40.2 KDA protein from Pyrococcus horikoshii (348 aa), FASTA scores: opt: 934, E(): 3.3e-53, (40.5% identity in 343 aa overlap); and other hypothetical proteins. P95188 No intermediary metabolism and respiration P95188 P95188 GO:0051536,GO:0016829,GO:0055114,GO:0016740,GO:0043365 1.97.1.4 P95188 NC_000962.3 Mycobrowser_v5 CDS 3511682 3512068 . + 0 Rv3145 nuoA Involved in aerobic|anaerobic respiration [catalytic activity: NADH + ubiquinone = NAD(+) + ubiquinol]. Probable NADH dehydrogenase I (chain A) NuoA (NADH-ubiquinone oxidoreductase chain A) Rv3145, (MTCY03A2.13c), len: 128 aa. Probable nuoA, integral membrane NADH dehydrogenase, chain A, similar to others e.g. Q9XAQ4|NUOA from Streptomyces coelicolor (119 aa), FASTA scores: opt: 405, E(): 5.4e-20, (68.75% identity in 128 aa overlap); Q9RU86|DR1506 from Deinococcus radiodurans (160 aa), FASTA scores: opt: 327, E(): 9e-15, (40.3% identity in 124 aa overlap); BAB47039|NDHC from Triticum aestivum (Wheat), FASTA scores: opt: 273, E(): 2.6e-11, (38.1% identity in 126 aa overlap); etc. Also similar to a NADH-plastoquinone oxidoreductases e.g. P26303|NU3C_WHEAT|NDHC from Triticum aestivum (Wheat) (120 aa), FASTA scores: opt: 273, E(): 2.6e-1, (38.1% identity in 126 aa overlap). Belongs to the complex I subunit 3 family. P9WIW7 No intermediary metabolism and respiration P65563 P9WIW7 GO:0016021,GO:0055114,GO:0019684,GO:0005886,GO:0048038,GO:0006810,GO:0008137 1.6.99.5 P9WIW7 NC_000962.3 Mycobrowser_v5 CDS 3573731 3577036 . - 0 Rv3201c Rv3201c Has both ATPase and helicase activities Probable ATP-dependent DNA helicase Rv3201c, (MTV014.45c), len: 1101 aa. Probable ATP-dependent DNA helicase, similar to others e.g. Q9FCK4|2SC3B6.08 from Streptomyces coelicolor (1222 aa), FASTA scores: opt: 1209, E(): 5.4e-63, (38.45% identity in 1199 aa overlap); P71561|PCRA_MYCTU|CRA|IVRD|Rv0949|MT0976|MTCY10D7.25c from Mycobacterium tuberculosis (771 aa), FASTA scores: opt: 403, E(): 6.5e-16, (28.15% identity in 717 aa overlap); Q9FCK5|2SC3B6.07 from Streptomyces coelicolor (1159 aa), FASTA scores: opt: 349, E(): 1.3e-12, (29.2% identity in 1144 aa overlap); Q9L3M1|UVRD from Prochlorococcus sp. (512 aa; fragment), FASTA scores: opt: 290, E(): 2e-09, (27.95% identity in 479 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). O53347 No information pathways O53347 O53347 GO:0004003,GO:0003677,GO:0006281,GO:0005524 3.6.1.- O53347 NC_000962.3 Mycobrowser_v5 CDS 3649420 3650109 . + 0 Rv3268 Rv3268 Function unknown Conserved hypothetical protein Rv3268, (MTCY71.08), len: 229 aa. Conserved hypothetical protein, similar to Q9KZK4|SCE34.22 hypothetical 27.1 KDA protein from Streptomyces coelicolor (263 aa), FASTA scores: opt: 442, E(): 5.9e-20, (40.1% identity in 242 aa overlap). Also weak similarity to N-terminal part (approximately 1530 to 1740 residues) of O07944|SNBDE pristinamycin I synthase 3 and 4 from Streptomyces pristinaespiralis (4848 aa), FASTA scores: opt: 159, E(): 0.11, (30.35% identity in 224 aa overlap). P96873 No conserved hypotheticals P96873 P96873 NC_000962.3 Mycobrowser_v5 CDS 3506790 3507995 . + 0 Rv3140 fadE23 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE23 Rv3140, (MTCY03A2.18c), len: 401 aa. Probable fadE23, acyl-CoA dehydrogenase (1.3.99.-) (see citation below), equivalent to O32891|MLCB1779.31|FADE23|ML0660 putative acyl-CoA dehydrogenase from Mycobacterium leprae (400 aa), FASTA scores: opt: 2307, E(): 3e-136, (89.5% identity in 401 aa overlap). Also similar to others e.g. Q9HUH1|PA4994 from Pseudomonas aeruginosa (402 aa), FASTA scores: opt: 1558, E(): 1.2e-89, (61.0% identity in 400 aa overlap); O31251 from Acinetobacter sp. ADP1 (401 aa), FASTA scores: opt: 1509, E(): 1.3e-86, (58.2% identity in 402 aa overlap); Q9K6D1|ACDA or BH3798 from Bacillus halodurans (380 aa), FASTA scores: opt: 612, E(): 8.4e-31, (38.2% identity in 293 aa overlap); Q9AHX9|FADFX from Pseudomonas putida (375 aa), FASTA scores: opt: 584, E(): 4.6e-29, (32.7% identity in 379 aa overlap); etc. Could belong to the acyl-CoA dehydrogenases family. P95186 No lipid metabolism P95186 P95186 GO:0055114,GO:0003995 P95186 NC_000962.3 Mycobrowser_v5 CDS 3509654 3510055 . + 0 Rv3143 Rv3143 Unknown, but could be involved in regulatory mechanism Probable response regulator Rv3143, (MTCY03A2.15c), len: 133 aa. Probable response regulator, similar to other sensory transduction regulatory proteins e.g. Q9X810|SC6G10.25 from Streptomyces coelicolor (133 aa), FASTA scores: opt: 474, E(): 2.8e-24, (54.15% identity in 120 aa overlap); Q9KZ82|SCE25.04c from Streptomyces coelicolor (225 aa), FASTA scores: opt: 144, E(): 0.016, (32.3% identity in 127 aa overlap); Q9RZT4|DRB0029 from Deinococcus radiodurans (416 aa), FASTA scores: opt: 145, E(): 0.024, (30.65% identity in 124 aa overlap). Similar to other regulatory components of sensory transduction systems. P9WGL7 No regulatory proteins P9WGL7 GO:0000156,GO:0000160,GO:0006355 P9WGL7 NC_000962.3 Mycobrowser_v5 CDS 3512077 3512631 . + 0 Rv3146 nuoB Involved in aerobic|anaerobic respiration [catalytic activity: NADH + ubiquinone = NAD(+) + ubiquinol]. Probable NADH dehydrogenase I (chain B) NuoB (NADH-ubiquinone oxidoreductase chain B) Rv3146, (MTCY03A2.12c), len: 184 aa. Probable nuoB, NADH dehydrogenase, chain B, similar to others e.g. Q9XAQ5|NUOB from Streptomyces coelicolor (184 aa), FASTA scores: opt: 989, E(): 1.4e-56, (78.25% identity in 184 aa overlap); Q56218|NQO6_THETH|NQO6 from Thermus aquaticus (subsp. thermophilus) (181 aa), FASTA scores: opt: 720, E(): 2.6e-39, (64.45% identity in 152 aa overlap); Q9RU87|DR1505 from Deinococcus radiodurans (181 aa), FASTA scores: opt: 719, E(): 3e-39, (62.6% identity in 155 aa overlap); etc. Belongs to the complex I 20 KDA subunit family. May contain an iron-sulfur 4FE-4S cluster. P9WJH1 No intermediary metabolism and respiration P65575 P9WJH1 GO:0008137,GO:0005506,GO:0055114,GO:0019684,GO:0005886,GO:0048038,GO:0006810,GO:0051539 1.6.99.5 P9WJH1 NC_000962.3 Mycobrowser_v5 CDS 3553039 3554025 . + 0 Rv3187 Rv3187 Involved in the transposition of the insertion sequence IS6110. Probable transposase Rv3187, (MTV014.31), len: 328 aa. Probable IS6110 transposase. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv3186 and Rv3187, the sequence UUUUAAAG (directly upstream of Rv3187) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 16 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WKH9 No insertion seqs and phages P0C5G8 P9WKH9 GO:0015074,GO:0006310,GO:0032196,GO:0003677 P9WKH9 NC_000962.3 Mycobrowser_v5 CDS 3513338 3514660 . + 0 Rv3148 nuoD Involved in aerobic|anaerobic respiration [catalytic activity: NADH + ubiquinone = NAD(+) + ubiquinol]. Probable NADH dehydrogenase I (chain D) NuoD (NADH-ubiquinone oxidoreductase chain D) Rv3148, (MTCY03A2.10c), len: 440 aa. Probable nuoD, NADH dehydrogenase, chain B, similar to others e.g. Q9XAQ7|NUOD from Streptomyces coelicolor (440 aa), FASTA scores: opt: 2198, E(): 1e-131, (73.9% identity in 429 aa overlap); P15689|NUCM_PARTE from Paramecium tetraurelia (400 aa), FASTA scores: opt: 922, E(): 5.8e-51, (38.5% identity in 408 aa overlap); Q9RU89|NUOD_DEIRA|DR1503 from Deinococcus radiodurans (401 aa), FASTA scores: opt: 922, E(): 5.8e-51, (47.75% identity in 404 aa overlap); etc. Equivalent to AAK47575 from Mycobacterium tuberculosis strain CDC1551 but longer 42 aa. Contains helix-turn-helix motif at aa 340-361. Belongs to the complex I 49 KDA subunit family. P9WJH5 No intermediary metabolism and respiration P65569 P9WJH5 GO:0051287,GO:0050136,GO:0055114,GO:0019684,GO:0005886,GO:0048038,GO:0006810 1.6.99.5 P9WJH5 NC_000962.3 Mycobrowser_v5 CDS 3514657 3515415 . + 0 Rv3149 nuoE Involved in aerobic|anaerobic respiration [catalytic activity: NADH + ubiquinone = NAD(+) + ubiquinol]. Probable NADH dehydrogenase I (chain E) NuoE (NADH-ubiquinone oxidoreductase chain E) Rv3149, (MTCY03A2.09c), len: 252 aa. Probable nuoE, NADH dehydrogenase, chain E, similar to others e.g. Q9XAQ8|NUOE from Streptomyces coelicolor (290 aa), FASTA scores: opt: 1002, E(): 5.7e-55, (69.5% identity in 213 aa overlap); P40915|NUHM_NEUCR|NUO-24 from Neurospora crassa (263 aa), FASTA scores: opt: 412, E(): 1.9e-18, (38055% identity in 192 aa overlap); P19234|NUHM_RAT from Rattus norvegicus (Rat) (241 aa), FASTA scores: opt: 410, E(): 2.4e-18, (23.9% identity in 237 aa overlap); etc. Belongs to the complex I 24 KDA subunit family. Binds a 2FE-2S cluster (potential). P9WIV5 No intermediary metabolism and respiration P65573 P9WIV5 GO:0051287,GO:0050136,GO:0046872,GO:0055114,GO:0048038,GO:0051537 1.6.99.5 P9WIV5 NC_000962.3 Mycobrowser_v5 CDS 3516746 3519166 . + 0 Rv3151 nuoG Involved in aerobic|anaerobic respiration [catalytic activity: NADH + ubiquinone = NAD(+) + ubiquinol]. Probable NADH dehydrogenase I (chain G) NuoG (NADH-ubiquinone oxidoreductase chain G) Rv3151, (MTCY03A2.07c), len: 806 aa. Probable nuoG, NADH dehydrogenase I, chain G, similar to others e.g. Q9XAR0|NUOG_STRCO from Streptomyces coelicolor (843 aa), FASTA scores: opt: 1968 ,E(): 5.2e-107, (62.45% identity in 818 aa overlap); P56914|NUG2_RHIME from Rhizobium meliloti (853 aa), FASTA scores: opt: 964, E(): 1.6e-48, (30.6% identity in 840 aa overlap); etc. But also similarity with other proteins e.g. P77908|FDHA formate dehydrogenase, alpha subunit (formate dehydrogenase [NADP+]) from Moorella thermoacetica (Clostridium thermoaceticum) (893 aa), FASTA scores: opt: 928, E(): 2e-46, (28.65% identity in 865 aa overlap); and Q9UUU3|NUAM NUAM protein precursor from Yarrowia lipolytica (Candida lipolytica) (728 aa), FASTA scores: opt: 894, E(): 1.7e-44, (31.95% identity in 676 aa overlap). Equivalent to AAK47578 from Mycobacterium tuberculosis strain CDC1551 but longer 15 aa. Contains respiratory-chain NADH dehydrogenase 75 kDa subunit signature 2 (PS00642). Belongs to the complex I 75 KDA subunit family. Cofactor: may bind two 4FE-4S cluster and one 2FE-2S cluster. P9WIV9 No intermediary metabolism and respiration P95175 P9WIV9 GO:0051539,GO:0042773,GO:0008137,GO:0009055,GO:0016020,GO:0030151,GO:0048038,GO:0051537 1.6.99.5 P9WIV9 NC_000962.3 Mycobrowser_v5 CDS 4375762 4375995 . - 0 Rv3893c PE36 Function unknown PE family protein PE36 Rv3893c, (MTCY15F10.19), len: 77 aa. PE36, Member of the Mycobacterium tuberculosis PE family of conserved proteins (see citation below), similar to others e.g. O53690|Rv0285|MTV035.13 from Mycobacterium tuberculosis (102 aa), FASTA scores: opt: 136, E(): 0.042, (35.6% identity in 73 aa overlap). L7N660 No PE/PPE Q7D4N3 L7N660 L7N660 NC_000962.3 Mycobrowser_v5 CDS 3520507 3521142 . + 0 Rv3153 nuoI Involved in aerobic|anaerobic respiration [catalytic activity: NADH + ubiquinone = NAD(+) + ubiquinol]. Probable NADH dehydrogenase I (chain I) NuoI (NADH-ubiquinone oxidoreductase chain I) Rv3153, (MTCY03A2.05c), len: 211 aa. Probable nuoI, NADH dehydrogenase I, chain I, similar to others e.g. Q9XAR2|NUOI from Streptomyces coelicolor (211 aa), FASTA scores: opt: 825, E(): 9.3e-44, (70.1% identity in 164 aa overlap); Q56224|NQO9_THETH from Thermus aquaticus (subsp. thermophilus) (182 aa), FASTA scores: opt: 543, E(): 1.8e-26, (50.9% identity in 163 aa overlap); Q9RU95|DR1497 from Deinococcus radiodurans (178 aa), FASTA scores: opt: 527, E(): 1.7e-25, (48.75% identity in 162 aa overlap); etc. Contains two 4Fe-4S ferredoxins, iron-sulfur binding region signatures (PS00198). Belongs to the complex I 23 KDA subunit family. The iron-sulfur centers are similar to those of 'bacterial-type' 4FE-4S ferredoxins. Cofactor: binds two 4FE-4S clusters. P9WJG9 No intermediary metabolism and respiration P95173 P9WJG9 GO:0050136,GO:0009055,GO:0005506,GO:0055114,GO:0019684,GO:0005886,GO:0048038,GO:0051539 1.6.99.5 P9WJG9 NC_000962.3 Mycobrowser_v5 CDS 3534392 3535351 . - 0 Rv3166c Rv3166c Function unknown Conserved hypothetical protein Rv3166c, (MTV014.10c), len: 319 aa. Probable transmembrane protein, similar but longer (52 aa) to O32895|MLCB1779.35c hypothetical protein from Mycobacterium leprae (119 aa), FASTA scores: opt: 289, E(): 3.7e-10, (44.25% identity in 122 aa overlap). Also some similarity to Q9Z536|SC9B2.23c putative transmembrane protein from Streptomyces coelicolor (339 aa), FASTA scores: opt: 247, E(): 2.5e-07, (28.2% identity in 326 aa overlap); and in N-terminus to Q9RS20|DR2307 putative multidrug-efflux transporter from Deinococcus radiodurans (410 aa), FASTA scores: opt: 135,E(): 1, (32.35% identity in 136 aa overlap). O53316 No conserved hypotheticals O53316 NC_000962.3 Mycobrowser_v5 CDS 3554298 3554645 . + 0 Rv3188 Rv3188 Function unknown Conserved hypothetical protein Rv3188, (MTV014.32), len: 115 aa. Conserved hypothetical protein, with similarity to other proteins from Mycobacterium tuberculosis: Q10868|YJ90_MYCTU|Rv1990c|MT2044|MTCY39.29 hypothetical protein (113 aa), FASTA scores: opt: 184, E(): 8.1e-06, (28.45% identity in 109 aa overlap); and O06299|Rv0348|MTCY13E10.08 hypothetical protein (217 aa), FASTA scores: opt: 129, E(): 0.074, (30.0% identity in 100 aa overlap). Also some similarity with C-terminus of Q9XA59|SCGD3.19 putative two-component system response transcriptional regulator from Streptomyces coelicolor (218 aa), FASTA scores: opt: 114, E(): 0.76, (30.0% identity in 110 aa overlap) (for this one, no similarity exists in the N-terminal region with the N-terminus of other regulatory components of sensory transduction systems). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53334 No conserved hypotheticals O53334 NC_000962.3 Mycobrowser_v5 CDS 3753765 3754256 . + 0 Rv3348 Rv3348 Possibly involved in the transposition of the insertion sequence IS1608'. Probable transposase Rv3348, (MTV004.04), len: 163 aa. Probable transposase, partially similar to several insertion elements e.g. P19834|YI11_STRCL insertion element IS116 hypothetical 44.8 KDA protein (similar to IS900 of Mycobacterium paratuberculosis) from Streptomyces clavuligerus (399 aa), FASTA scores: opt: 146, E(): 0.016, (29.1% identity in 158 aa overlap). P96234 No insertion seqs and phages P96234 P96234 GO:0004803,GO:0006313,GO:0003677 NC_000962.3 Mycobrowser_v5 CDS 3874404 3874736 . + 0 Rv3453 Rv3453 Unknown Possible conserved transmembrane protein Rv3453, (MTCY13E12.06), len: 110 aa. Possible conserved transmembrane protein, showing weak similarity with other proteins e.g. Q9F6C3 putative ABC transporter from Propionibacterium thoenii (424 aa), FASTA scores: opt: 104, E(): 6.8, (40.6% identity in 69 aa overlap). O06320 No cell wall and cell processes O06320 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 3524132 3525793 . + 0 Rv3157 nuoM Involved in aerobic|anaerobic respiration [catalytic activity: NADH + ubiquinone = NAD(+) + ubiquinol]. Probable NADH dehydrogenase I (chain M) NUOK (NADH-ubiquinone oxidoreductase chain M) Rv3157, (MTCY03A2.01c-MTV014.01c), len: 553 aa. Probable nuoM, integral membrane NADH dehydrogenase I, chain M, similar to others e.g. Q9XAR6|NUOM from Streptomyces coelicolor (523 aa), FASTA scores: opt: 1621, E(): 4.2e-89, (56.55% identity in 541 aa overlap); P50974|NUOM_RHOCA|NUOM from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (512 aa), FASTA scores: opt: 996, E(): 6.5e-52, (38.2% identity in 521 aa overlap); P29925|NQOD_PARDE|NQO13 from Paracoccus denitrificans (513 aa), FASTA scores: opt: 987, E(): 2.2e-51, (37.05% identity in 540 aa overlap); etc. Also similar to MTCY251.04 (FASTA score: E(): 3.3e-16) and MTCY03A2.02c (FASTA score: E(): 9.6e-13). Similar to polypeptide 4 of the NADH-ubiquinol oxidoreductase of chloroplasts or mitochondrial. P9WIW5 No intermediary metabolism and respiration O53307 P9WIW5 GO:0008137,GO:0016021,GO:0005886,GO:0048038,GO:0042773 1.6.99.5 NC_000962.3 Mycobrowser_v5 CDS 3551556 3552542 . + 0 Rv3185 Rv3185 Involved in the transposition in the insertion sequence IS6110. Probable transposase Rv3185, (MTV014.29), len: 328 aa. Probable IS6110 transposase. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv3184 and Rv3185, the sequence UUUUAAAG (directly upstream of Rv3185) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 16 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WKH9 No insertion seqs and phages P0C5G8 P9WKH9 GO:0015074,GO:0006310,GO:0032196,GO:0003677 P9WKH9 NC_000962.3 Mycobrowser_v5 CDS 3531642 3532913 . - 0 Rv3163c Rv3163c Unknown Possible conserved secreted protein Rv3163c, (MTV014.07c), len: 423 aa. Possible conserved secreted protein, with some similarity to other hypothetical bacterial proteins e.g. Q9Z539|SC9B2.20c from Streptomyces coelicolor (460 aa), FASTA scores: opt: 666, E(): 1.5e-33, (33.55% identity in 417 aa overlap); O58486|PH0774 from Pyrococcus horikoshii (410 aa), FASTA scores: opt: 329, E(): 6.9e-13, (23.8% identity in 424 aa overlap); Q9UZ66|PAB0849 from Pyrococcus abyssi (410 aa), FASTA scores: opt: 322, E(): 1.9e-12, (24.15% identity in 389 aa overlap); etc. Also some similarity with P71761|Rv1480|MTV007.27|MTCY277.01 from Mycobacterium tuberculosis (317 aa), FASTA scores: opt: 198, E(): 6.3e-05, (26.75% identity in 269 aa overlap). Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature. O53313 No cell wall and cell processes O53313 O53313 O53313 NC_000962.3 Mycobrowser_v5 CDS 3532943 3533905 . - 0 Rv3164c moxR3 Involved in transcriptional mechanism; regulates methanol dehydrogenase. Probable methanol dehydrogenase transcriptional regulatory protein MoxR3 Rv3164c, (MTV014.08c), len: 320 aa. Probable moxR3, methanol dehydrogenase regulatory protein, highly similar to Q9Z538|SC9B2.21c putative regulatory protein from Streptomyces coelicolor (332 aa), FASTA scores: opt: 1227, E(): 1.7e-67, (60.25% identity in 302 aa overlap); Q9UZ67|MOXR-3|PAB0848 methanol dehydrogenase regulatory protein from Pyrococcus abyssi (314 aa), FASTA scores: opt: 1126, E(): 2.3e-61, (54.1% identity in 305 aa overlap); Q9HSH7|MOXR|VNG0223G methanol dehydrogenase regulatory protein from Halobacterium sp. strain NRC-1 (318 aa), FASTA scores: opt: 1072, E(): 4.5e-58, (51.45% identity in 315 aa overlap); Q9RVV4|DR0918 MOXR-related protein from Deinococcus radiodurans (354 aa), FASTA scores: opt: 1000, E(): 1.2e-53, (50.95% identity in 318 aa overlap); etc. Also high similarity with several hypothetical bacterial proteins. O53314 No regulatory proteins O53314 O53314 GO:0016887,GO:0005524 O53314 NC_000962.3 Mycobrowser_v5 CDS 3527391 3529163 . - 0 Rv3159c PPE53 Function unknown PPE family protein PPE53 Rv3159c, (MTV014.03c), len: 590 aa. PPE53, Member of the Mycobacterium tuberculosis PPE_family of Gly-, Asn-rich proteins. Highly similar to P71868|Rv3533c|MTCY03C7.23 (582 aa), FASTA scores: opt: 2289, E(): 3.2e-98, (63.5% identity in 600 aa overlap); and also similar to MTCY48_17, MTV041_29, MTCY6G11_5, MTCY98_24, etc. Predicted to be an outer membrane protein (See Song et al., 2008). Q6MX04 No PE/PPE Q6MX04 Q6MX04 Q6MX04 NC_000962.3 Mycobrowser_v5 CDS 3529338 3529979 . - 0 Rv3160c Rv3160c Possibly involved in transcriptional mechanism. Possible transcriptional regulatory protein (probably TetR-family) Rv3160c, (MTV014.04c), len: 213 aa. Possible transcriptional regulator, with some similarity to others e.g. Q9S3L4|AMTR AMTR protein (global repressor in the nitrogen regulation system; see Jakoby et al., 2000) (222 aa), FASTA scores: opt: 182, E(): 7.3e-05, (27.9% identity in 208 aa overlap); Q9X7X9|SC6A5.33c putative regulatory protein from Streptomyces coelicolor (223 aa), FASTA scores: opt: 176, E(): 0.00018, (26.5% identity in 185 aa overlap); Q9XA31|SCH69.03c putative transcriptional regulator from Streptomyces coelicolor (209 aa), FASTA scores: opt: 173, E(): 0.00027, (27.25% identity in 176 aa overlap); BAB54133|MLL7734 transcriptional regulator from Rhizobium loti (Mesorhizobium loti) (213 aa), FASTA scores: opt: 172, E(): 0.00031, (23.55% identity in 204 aa overlap); etc. Also similar to hypothetical proteins from Mycobacterium tuberculosis strain H37Rv e.g. P96839|Rv3557v|MTCY06G11.04c (200 aa), FASTA scores: opt: 169, E(): 0.00046, (26.75% identity in 157 aa overlap). Contains probable helix-turn-helix motif from aa 31 to 52 (Score 1857, +5.51 SD). Similar to the TetR/AcrR family of transcriptional regulators. O53310 No regulatory proteins O53310 O53310 GO:0006350,GO:0003700,GO:0006355 O53310 NC_000962.3 Mycobrowser_v5 CDS 3529990 3531138 . - 0 Rv3161c Rv3161c Function unknown; involved in cellular metabolism. Possible dioxygenase Rv3161c, (MTV014.05c), len: 382 aa. Possible dioxygenase, similar to subunit of several dioxygenases and related proteins e.g. BAB50510|MLR3662 dioxygenase, alpha subunit from Rhizobium loti (Mesorhizobium loti) (400 aa), FASTA scores: opt: 413, E(): 6.2e-20, (28.4% identity in 331 aa overlap); Q9A3T0|CC3122 rieske 2FE-2S family protein from Caulobacter crescentus (404 aa), FASTA scores: opt: 405, E(): 2.1e-19, (27.95% identity in 372 aa overlap); Q9HTF4|PA5410 probable ring hydroxylating dioxygenase, alpha-subunit from Pseudomonas aeruginosa (429 aa), FASTA scores: opt: 392, E(): 1.6e-18, (25.8% identity in 399 aa overlap); Q9AGK6|PHTAA phthalate dioxygenase large subunit from Arthrobacter keyseri (473 aa), FASTA scores: opt: 385, E(): 5.2e-18, (34.0% identity in 206 aa overlap); P76253|YEAW_ECOLI putative dioxygenase, alpha subunit from Escherichia coli (374 aa), FASTA scores: opt: 376, E(): 1.7e-17, (27.05% identity in 344 aa overlap); etc. O53311 No intermediary metabolism and respiration O53311 O53311 GO:0006725,GO:0005506,GO:0055114,GO:0016702,GO:0051537 1.-.-.- O53311 NC_000962.3 Mycobrowser_v5 CDS 4131739 4132392 . + 0 Rv3690 Rv3690 Unknown Probable conserved membrane protein Rv3690, (MTV025.038), len: 217 aa. Probable conserved membrane protein, similar to Q9KYW5|SCE33.18 putative integral membrane protein from Streptomyces coelicolor (231 aa), FASTA scores: opt: 419, E(): 1.5e-19, (36.0% identity in 211 aa overlap). Equivalent to AAK48159 from Mycobacterium tuberculosis strain CDC1551 (233 aa) but shorter 16 aa. O69658 No cell wall and cell processes O69658 NC_000962.3 Mycobrowser_v5 CDS 3536102 3537238 . + 0 Rv3168 Rv3168 Confers resistance to aminoglycoside antibiotics Putative aminoglycoside phosphotransferase Rv3168, (MTV014.12), len: 378 aa. Putative aminoglycoside phosphotransferase, similar to hypothetical proteins e.g. Q9M7Y6|F3E22.6 from Arabidopsis thaliana (Mouse-ear cress) (314 aa), FASTA scores: opt: 236, E(): 1.1e-07, (27.35% identity in 234 aa overlap); Q9RYW2|DRA0194 from Deinococcus radiodurans (386 aa), FASTA scores: opt: 207, E(): 9.1e-06, (23.45% identity in 320 aa overlap); etc. Also some similarity with O69727|Rc3761c|MTV025.109c hypothetical protein from Mycobacterium tuberculosis (351 aa), FASTA scores: opt: 193, E(): 6.4e-05, (29.4% identity in 242 aa overlap). P9WI99 No virulence, detoxification, adaptation 3ATS,3ATT O53318 P9WI99 P9WI99 NC_000962.3 Mycobrowser_v5 CDS 3537238 3538362 . + 0 Rv3169 Rv3169 Function unknown Conserved protein Rv3169, (MTV014.13), len: 374 aa. Conserved protein, with similarity to other hypothetical proteins: Q9A8W6|CC1232 from Caulobacter crescentus (368 aa), FASTA scores: opt: 669, E(): 3.3e-34, (34.05% identity in 376 aa overlap); and O32901|MLCB1779.41 from Mycobacterium leprae (127 aa), FASTA scores: opt: 179, E(): 0.00034, (29.0% identity in 131 aa overlap). Also weak similarity with P95149|Rv1866|MTCY359.07c (804 aa), FASTA scores: opt: 121, E(): 6.4, (37.0% identity in 119 aa overlap). Equivalent to AAK47597 from Mycobacterium tuberculosis strain CDC1551 but shorter 43 aa. O53319 No conserved hypotheticals O53319 O53319 NC_000962.3 Mycobrowser_v5 CDS 3554642 3555262 . + 0 Rv3189 Rv3189 Function unknown Conserved hypothetical protein Rv3189, (MTV014.33), len: 206 aa. Conserved hypothetical protein, weakly similar to other proteins from Mycobacterium tuberculosis e.g. O86329|MBTE|Rv2380c|MTCY22H8.05 (1682 aa), FASTA scores: opt: 135, E(): 0.79, (27.8% identity in 187 aa overlap); and Q10869|YJ89_MYCTU|Rv1989c|MT2043MTCY39.30 (186 aa), FASTA scores: opt: 122, E(): 0.85, (32.25% identity in 93 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53335 No conserved hypotheticals O53335 O53335 O53335 NC_000962.3 Mycobrowser_v5 CDS 4318775 4319266 . + 0 Rv3844 Rv3844 Required for the transposition of an insertion sequence. Possible transposase Rv3844, (MTCY01A6.25), len: 163 aa. Possible transposase, identical to P96234|Rv3348|MTV004.04 putative transposase from Mycobacterium tuberculosis. Also some similarity with others e.g. N-terminal part of P19834|YI11_STRCL insertion element IS116 hypothetical 44.8 KDA protein from Streptomyces clavuligerus (399 aa) FASTA scores: opt: 146, E(): 0.017, (29.1% identity in 158 aa overlap). P96234 No insertion seqs and phages P96234 P96234 GO:0004803,GO:0006313,GO:0003677 NC_000962.3 Mycobrowser_v5 CDS 3542138 3542845 . + 0 Rv3174 Rv3174 Function unknown; involved in cellular metabolism. Probable short-chain dehydrogenase/reductase Rv3174, (MTV014.18), len: 235 aa. Probable oxidoreductase short-chain dehyrogenase/reductase, similar to others e.g. Q9RPT7|sits from Streptomyces albus (223 aa), FASTA scores: opt: 654, E(): 6.1e-32, (49.3% identity in 215 aa overlap); Q9RI61|SCJ11.46 from Streptomyces coelicolor (230 aa), FASTA scores: opt: 626, E(): 2.9e-30, (50.9% identity in 224 aa overlap); Q9A5Z1|CC2306 from Caulobacter crescentus (252 aa), FASTA scores: opt: 430, E(): 1.3e-18, (39.45% identity in 228 aa overlap); Q51641 insect-type dehydrogenase (249 aa), FASTA scores: opt: 301, E(): 5.7e-11, (38.3% identity in 188 aa overlap); Q9HXC9|PA3883 from Pseudomonas aeruginosa (276 aa), FASTA scores: opt: 296, E(): 1.2e-10, (29.55% identity in 247 aa overlap); etc. May belong to the short-chain dehydrogenases/reductases (SDR) family. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53324 No intermediary metabolism and respiration O53324 O53324 GO:0055114,GO:0016491 1.-.-.- O53324 NC_000962.3 Mycobrowser_v5 CDS 3551281 3551607 . + 0 Rv3184 Rv3184 Involved in the transposition in the insertion sequence IS6110. Probable transposase for insertion sequence element IS6110 (fragment) Rv3184, (MTV014.28), len: 108 aa. Putative Transposase for IS6110 (fragment). Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv3184 and Rv3185, the sequence UUUUAAAG (directly upstream of Rv3185) maybe responsible for such a frameshifting event (see McAdam et al., 1990). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WKH5 No insertion seqs and phages P0C5G9 P9WKH5 GO:0004803,GO:0006313,GO:0003677 P9WKH5 NC_000962.3 Mycobrowser_v5 CDS 3555422 3556687 . - 0 Rv3190c Rv3190c Unknown Hypothetical protein Rv3190c, (MTV014.34c), len: 421 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53336 No conserved hypotheticals O53336 NC_000962.3 Mycobrowser_v5 CDS 3545447 3546307 . + 0 Rv3177 Rv3177 Supposedly involved in detoxification reactions. Possible peroxidase (non-haem peroxidase) Rv3177, (MTV014.21), len: 286 aa. Possible peroxidase (non-haem peroxidase), highly similar to Q9KJF9|W78 cultivar specificity protein (similar to alpha/beta hydrolase fold) W78 from Rhizobium leguminosarum (287 aa), FASTA scores: opt: 1059, E(): 2.3e-59, (61.4% identity in 272 aa overlap); BAB48728|MLL1328 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (286 aa), FASTA scores: opt: 746, E(): 1.1e-39, (43.25% identity in 282 aa overlap). Similar to nonheme chloroperoxidases and related esterases e.g. O73957|SAL lipolytic enzyme from Sulfolobus acidocaldarius (314 aa), FASTA scores: opt: 408, E(): 1.9e-18, (32.4% identity in 287 aa overlap); Q9AJM9|BIOH protein involved in biotin synthesis from Kurthia sp. 538-KA26 (267 aa), FASTA scores: opt: 324 ,E(): 3.2e-13, (30.0% identity in 250 aa overlap); Q9CBB1|ML2269 putative hydrolase (similar to alpha/beta hydrolase fold) from Mycobacterium leprae (265 aa); O05691|THCF_RHOER non-heme haloperoxidase from Rhodococcus erythropolis (similar to other bacterial non-heme BROMO- and chloro-peroxidases) (274 aa), FASTA scores: opt: 279, E(): 2.2e-10, (29.0% identity in 276 aa overlap); Q53540|est esterase (similar to alpha/beta hydrolase fold) from Pseudomonas putida (276 aa), FASTA scores: opt: 271, E(): 7.1e-10, (29.65% identity in 280 aa overlap); etc. Also similar to O06420|BPOC|Rv0554|MTCY25D10.33 hypothetical 28.3 KDA protein (similar to alpha/beta hydrolase fold) from M. tuberculosis (262 aa), FASTA scores: opt: 280 ,E(): 1.8e-10, (28.0% identity in 257 aa overlap). Equivalent to AAK47605 from Mycobacterium tuberculosis strain CDC1551 (300 aa) but shorter 14 aa. Similar to alpha/beta hydrolase fold. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53327 No virulence, detoxification, adaptation O53327 O53327 GO:0004601,GO:0055114 1.11.1.- O53327 NC_000962.3 Mycobrowser_v5 CDS 3546438 3546797 . + 0 Rv3178 Rv3178 Function unknown Conserved hypothetical protein Rv3178, (MTV014.22), len: 119 aa. Hypothetical protein, with some similarity to other hypothetical bacterial proteins (principally mycobacterium and streptomyces proteins) e.g. P71854|Rv3547|MTCY03C7.09c from Mycobacterium tuberculosis strain H37Rv (151 aa), FASTA scores: opt: 310, E(): 2e-14, (40.5% identity in 116 aa overlap); Q9ZH81 from M. paratuberculosis (144 aa), FASTA scores: opt: 274, E(): 5.6e-12, (38.9% identity in 108 aa overlap); O85698|3SCF60.07 from Streptomyces lividans and Streptomyces coelicolor (149 aa), FASTA scores: opt: 235, E(): 2.7e-09, (35.2% identity in 108 aa overlap); Q10772|YF58_MYCTU|Rv1558|MT1609|MTCY48.07c (148 aa); Q9WX21|SCE68.11 from Streptomyces coelicolor (305 aa); etc. Equivalent to AAK47606 from Mycobacterium tuberculosis strain CDC1551 (171 aa) but shorter 52 aa. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53328 No conserved hypotheticals O53328 O53328 O53328 NC_000962.3 Mycobrowser_v5 CDS 3547618 3548907 . + 0 Rv3179 Rv3179 Function unknown Conserved protein Rv3179, (MTV014.23), len: 429 aa. Conserved protein, highly similar to Q9KH61 putative ATP/GTP binding protein from Mycobacterium smegmatis (428 aa), FASTA scores: opt: 2466, E(): 1.5e-148, (89.7% identity in 428 aa overlap) (no article found on the NCBI web site (July 2001)); and to other hypothetical bacterial proteins e.g. O07781|Rv0597c|MTCY19H5.25 from M. tuberculosis (411 aa), FASTA scores: opt: 1031, E(): 8e-58, (41.5% identity in 417 aa overlap); BAB54715|MLR9349 from Rhizobium loti (Mesorhizobium loti) (435 aa), FASTA scores: opt: 365, E(): 1.1e-15, (31.75% identity in 416 aa overlap); etc. Equivalent to AAK47609 from Mycobacterium tuberculosis strain CDC1551 (454 aa) but shorter 25 aa. Contains PS00017 ATP/GTP-binding site motif A (P-loop). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53329 No conserved hypotheticals O53329 O53329 NC_000962.3 Mycobrowser_v5 CDS 3552764 3553090 . + 0 Rv3186 Rv3186 Involved in the transposition of the insertion sequence IS6110. Probable transposase for insertion sequence element IS6110 (fragment) Rv3186, (MTV014.30), len: 108 aa. Putative Transposase for IS6110 (fragment). Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv3186 and Rv3187, the sequence UUUUAAAG (directly upstream of Rv3187) maybe responsible for such a frameshifting event (see McAdam et al., 1990). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WKH5 No insertion seqs and phages P0C5G9 P9WKH5 GO:0004803,GO:0006313,GO:0003677 P9WKH5 NC_000962.3 Mycobrowser_v5 CDS 3849294 3850139 . - 0 Rv3431c Rv3431c Involved in the transposition of the insertion sequence IS1552 Possible transposase (fragment) Rv3431c, (MTCY77.03c), len: 281 aa. Possible truncated transposase for IS1552, similar to, but shorter than other transposases e.g. P72303 from Rhodococcus opacus (418 aa), FASTA scores: opt: 1509, E(): 1.2e-91, (80.95% identity in 278 aa overlap); Q9AKV5 from Mycobacterium paratuberculosis (395 aa), FASTA scores: opt: 1115, E(): 7.8e-66, (63.45% identity in 268 aa overlap); etc. I6XH73 No insertion seqs and phages O06248 I6XH73 GO:0004803,GO:0006313,GO:0003677 I6XH73 NC_000962.3 Mycobrowser_v5 tRNA 3559370 3559443 . - 0 MTB000043 metU - tRNA-fMet (CAT) metU, tRNA-fMet, anticodon cat, length = 74. Described in EM_BA: MTMETA Y08623 M.tuberculosis as metA gene. Name changed to metU as metA encodes homoserine transsuccinylase. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 3559563 3560024 . + 0 Rv3192 Rv3192 Function unknown Conserved hypothetical alanine and proline-rich protein Rv3192, (MTV014.36), len: 153 aa. Conserved hypothetical ala- and pro-rich protein, with weak similarity to N-terminal half of several proteins e.g. Q11030|YD60_MYCTU|Rv1360|MT1405|MTCY02B10.24 hypothetical 37.3 KDA protein from Mycobacterium tuberculosis (340 aa), FASTA scores: opt: 245, E(): 3.7e-08, (33.1% identity in 157 aa overlap); O30260|AF2411 conserved hypothetical protein from Archaeoglobus fulgidus (363 aa), FASTA scores: opt: 144, E(): 0.072, (32.6% identity in 92 aa overlap); Q9ZA30|GRA-ORF29 putative FMN-dependent monooxygenase from Streptomyces violaceoruber (343 aa), FASTA scores: opt: 133, E(): 0.33, (25.15% identity in 159 aa overlap). O53338 No conserved hypotheticals O53338 O53338 GO:0016491,GO:0055114 O53338 NC_000962.3 Mycobrowser_v5 CDS 3569109 3571211 . - 0 Rv3198c uvrD2 Involved in nucleotide excision repair. Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the postincision events of nucleotide excision repair and methyl-directed mismatch repair. Probable ATP-dependent DNA helicase II UvrD2 Rv3198c, (MTV014.42c), len: 700 aa. Probable UvrD2, ATP dependent DNA helicase II (see citation below), equivalent to P53528|UVRD_MYCLE|VRD|UVRD2|ML0637|B1937_F1_27 probable DNA helicase II homolog from Mycobacterium leprae (717 aa), FASTA scores: opt: 3749, E(): 0, (82.85% identity in 706 aa overlap); and C-terminal half (466-700 aa) corresponds to Q49764|RECQ|B1937_F2_66 putative DNA helicase RECQ (242 aa), FASTA scores: opt: 1267, E(): 1.4e-69, (82.5% identity in 234 aa overlap); products of two adjacent ORFS in Mycobacterium leprae. Also similar to other DNA helicases e.g. Q9FCK0|2SC3B6.12 from Streptomyces coelicolor (785 aa), FASTA scores: opt: 1687, E(): 1.2e-94, (52.05% identity in 728 aa overlap); P71561|CRA|IVRD|Rv0949|MT0976|MTCY10D7.25c ATP-dependent DNA helicase PCRA from Mycobacterium tuberculosis (771 aa), FASTA scores: opt: 715, E(): 1e-35, (34.1% identity in 710 aa overlap); Q9CD72|PCRA_MYCLE|UVRD|ML0153 ATP-dependent DNA helicase PCRA from Mycobacterium leprae (778 aa), FASTA scores: opt: 687, E(): 5.1e-34, (32.0% identity in 719 aa overlap); O83991|TP1028 DNA helicase II (UVRD) from Treponema pallidum (670 aa), FASTA scores: opt: 652, E(): 6e-32, (30.25% identity in 671 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the UVRD subfamily of helicases. P9WMP9 No information pathways P64320 P9WMP9 GO:0004003,GO:0003677,GO:0006281,GO:0006260,GO:0005622,GO:0005524 3.6.4.12 P9WMP9 NC_000962.3 Mycobrowser_v5 CDS 3586844 3587539 . - 0 Rv3210c Rv3210c Function unknown Conserved protein Rv3210c, (MTCY07D11.16), len: 231 aa. Conserved protein, similar (but N-terminus shorter) to Q9FBJ1|SCP8.30 conserved hypothetical protein from Streptomyces coelicolor (260 aa), FASTA scores: opt: 599, E(): 1.1e-30, (42.5% identity in 233 aa overlap); and some similarity to Q9RRV1|DR2384 phenylacetic acid degradation protein PAAC from Deinococcus radiodurans (263 aa), FASTA scores: opt: 129, E(): 0.43, (27.9% identity in 172 aa overlap); and Q9F621 FLGK protein from Rhizobium meliloti (Sinorhizobium meliloti) (472 aa). O05856 No conserved hypotheticals O05856 GO:0016491,GO:0046914,GO:0055114 O05856 NC_000962.3 Mycobrowser_v5 CDS 4319281 4319640 . + 0 Rv3845 Rv3845 Unknown Hypothetical protein Rv3845, (MTCY01A6.24c), len: 119 aa. Hypothetical unknown protein. Contains PS01137 Hypothetical YBL055c/yjjV family signature 1. P96233 No conserved hypotheticals P96233 NC_000962.3 Mycobrowser_v5 CDS 3563264 3564286 . - 0 Rv3194c Rv3194c Function unknown Possible conserved secreted protein Rv3194c, (MTV014.38c), len: 340 aa. Possible conserved secreted protein (N-terminal stretch hydrophobic), equivalent to Q9CCM7|ML0643 putative secreted protein from Mycobacterium leprae (340 aa), FASTA scores: opt: 1822, E(): 1.6e-102, (80.3% identity in 340 aa overlap). Also similar to other proteins e.g. Q9FCI6|2SC3B6.26 putative secreted protein from Streptomyces coelicolor (364 aa), FASTA scores: opt: 430, E(): 1.1e-18, (40.95% identity in 359 aa overlap); Q9S3Y5|SDRC SDRC protein from Streptomyces coelicolor (241 aa), FASTA scores: opt: 396, E(): 8.9e-17, (35.2% identity in 318 aa overlap) (similarity in part for this one); O34470|YLBL YLBL protein from Bacillus subtilis (350 aa), FASTA scores: opt: 385, E(): 5.6e-16, (27.7% identity in 350 aa overlap); etc. O53340 No cell wall and cell processes O53340 O53340 GO:0005515,GO:0006508,GO:0004252,GO:0004176 O53340 NC_000962.3 Mycobrowser_v5 CDS 3564364 3565782 . + 0 Rv3195 Rv3195 Function unknown Conserved hypothetical protein Rv3195, (MTV014.39), len: 472 aa. Hypothetical protein, equivalent to Q49746|ML0642|B1937_C3_231 hypothetical 50.3 KDA protein from Mycobacterium leprae (479 aa), FASTA scores: opt: 2503, E(): 1e-138, (79.35% identity in 475 aa overlap). Similar in part to Q9FCI9|2SC3B6.23c conserved hypothetical protein from Streptomyces coelicolor (487 aa), FASTA scores: opt: 1382, E(): 2.7e-73, (46.4% identity in 489 aa overlap); Q9X8I7|SCE9.14 hypothetical 41.2 KDA protein from Streptomyces coelicolor (375 aa), FASTA scores: opt: 319, E(): 2.4e-11, (25.6% identity in 383 aa overlap); etc. O53341 No conserved hypotheticals O53341 O53341 O53341 NC_000962.3 Mycobrowser_v5 CDS 3565788 3566687 . + 0 Rv3196 Rv3196 Function unknown Conserved hypothetical protein Rv3196, (MTV014.40), len: 299 aa. Hypothetical protein, with some similarity to other hypothetical proteins e.g. Q9FCJ5|2SC3B6.17c putative secreted protein from Streptomyces coelicolor (442 aa), FASTA scores: opt: 233, E(): 3.5e-07, (29.9% identity in 261 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). O53342 No conserved hypotheticals O53342 O53342 NC_000962.3 Mycobrowser_v5 CDS 3581627 3582505 . - 0 Rv3205c Rv3205c Function unknown Conserved protein Rv3205c, (MTCY07D11.21), len: 292 aa. Conserved protein, highly similar to Q9CCG7|ML0818 hypothetical protein from Mycobacterium leprae (297 aa), FASTA scores: opt: 1745, E(): 9.1e-98, (87.3% identity in 291 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O05861 No conserved hypotheticals O05861 O05861 NC_000962.3 Mycobrowser_v5 CDS 3571335 3571589 . + 0 Rv3198A Rv3198A Function unknown Possible glutaredoxin protein Rv3198A, len: 84 aa. Possible glutaredoxin protein , highly similar to Q9FCK1|2SC3B6.11c putative glutaredoxin-like protein from Streptomyces coelicolor (80 aa), FASTA scores: opt: 293, E(): 2.2e-14, (55.15% identity in 78 aa overlap); and Q9RSN9|DR2085 putative glutaredoxin from Deinococcus radiodurans (81 aa), FASTA scores: opt: 198, E(): 1.2e-07, (53.55% identity in 56 aa overlap). Also similar to several hypothetical bacterial proteins e.g. Q9X8C2|SCE36.09 hypothetical 13.0 KDA protein from Streptomyces coelicolor (114 aa), FASTA scores: opt: 181, E(): 2.6e-06, (44.45% identity in 72 aa overlap). Q8VJ51 No intermediary metabolism and respiration Q8VJ51 Q8VJ51 GO:0009055,GO:0055114,GO:0015035,GO:0045454 1.-.-.- NC_000962.3 Mycobrowser_v5 CDS 3598353 3598904 . - 0 Rv3222c Rv3222c Function unknown Conserved hypothetical protein Rv3222c, (MTCY07D11.04), len: 183 aa. Hypothetical protein, with some similarity to Q9SZD2|F19B15.50|AT4G29020 glycine-rich protein like from Arabidopsis thaliana (Mouse-ear cress) (158 aa), FASTA scores: opt: 131, E(): 0.77, (33.35% identity in 126 aa overlap); Q9S222|SCI51.18 putative transcriptional regulator from Streptomyces coelicolor (548 aa), FASTA scores: opt: 133, E(): 1.6, (36.25% identity in 149 aa overlap); etc. Also some similarity to other hypothetical Mycobacterium tuberculosis proteins e.g. O06292|Rv0341|MTCY13E10.01 (479 aa), FASTA scores: opt: 141, E(): 0.5, (31.2% identity in 170 aa overlap); AAK45760|MT1497.1 PE_PGRS family protein from strain CDC1551 (1408 aa), FASTA scores: opt: 137, E(): 2, (31.75% identity in 148 aa overlap); etc. O05844 No conserved hypotheticals O05844 NC_000962.3 Mycobrowser_v5 CDS 3598901 3599551 . - 0 Rv3223c sigH Alternative sigma factor that plays a role in the oxidative-stress response (regulation of thioredoxin recycling). The sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing in the extracytoplasmic compartment. Regulates positively DNAK and CLPB genes. Regulates TRXB2, TRXC, Rv2466c and SIGB genes, and probably SIGB gene. SigH may mediate the transcription of at least 31 genes directly and modulates the expression of about 150 others. Alternative RNA polymerase sigma-E factor (sigma-24) SigH (RPOE) Rv3223c, (MTCY07D11.03), len: 216 aa. SigH (alternate gene name: rpoE), alternative RNA polymerase sigma factor (see citations below), similar to many e.g. Q9XCD8|sigh from Mycobacterium smegmatis (215 aa), FASTA scores: opt: 1187, E(): 8.1e-69, (87.75% identity in 212 aa overlap); O87834|SIGR from Streptomyces coelicolor (227 aa), FASTA scores: opt: 913, E(): 2.6e-51, (68.8% identity in 202 aa overlap); O68520|RPOE1 from Myxococcus xanthus (213 aa), FASTA scores: opt: 452, E(): 6.7e-22, (42.8% identity in 187 aa overlap); Q06198|RPSH_PSEAE|ALGU|ALGT|PA0762 from Pseudomonas aeruginosa (193 aa), FASTA scores: opt: 301, E(): 2.7e-12, (29.9% identity in 194 aa overlap); etc. Equivalent to AAK47662 RNA polymerase sigma-70 factor from Mycobacterium tuberculosis strain CDC1551 (284 aa), but shorter 68 aa. Has sigma-70 factors ECF subfamily signature (PS01063). So belongs to the sigma-70 factor family, ECF subfamily. Start chosen on basis of similarity, other potential starts upstream. P9WGH9 No information pathways P66807 P9WGH9 GO:0006950,GO:0016987,GO:0003700,GO:0006352,GO:0006355 P9WGH9 NC_000962.3 Mycobrowser_v5 CDS 3600801 3601019 . + 0 Rv3224B Rv3224B Function unknown Conserved hypothetical protein Rv3224B, len: 72 aa. Conserved hypothetical protein (possibly gene fragment), similar to C-terminal part of ML0799|AL583919_131 conserved hypothetical protein from Mycobacterium leprae (135 aa), FASTA scores: opt: 229, E(): 2e-09, (60.00% identity in 70 aa overlap). Note that downstream ORF Rv3224A is similar to N-terminus of ML0799. There appears to be no frameshift as sequence is identical in strain CDC1551 and in Mycobacterium bovis. Predicted to be an outer membrane protein (See Song et al., 2008). Q6MWZ4 No conserved hypotheticals Q6MWZ4 Q6MWZ4 Q6MWZ4 NC_000962.3 Mycobrowser_v5 CDS 4368518 4370170 . - 0 Rv3886c mycP2 Thought to have proteolytic activity. Probable alanine and proline rich membrane-anchored mycosin MycP2 (serine protease) (subtilisin-like protease) (subtilase-like) (mycosin-2) Rv3886c, (MTCY15F10.26), len: 550 aa. Probable mycP2, ala-, pro-rich membrane-anchored serine protease (mycosin) (see citation below), highly similar to Q9CBV3|ML1538 possible protease from Mycobacterium leprae (567 aa), FASTA scores: opt: 1034, E(): 3.9e-32, (43.5% identity in 575 aa overlap); and highly similar, but with gaps, to several putative proteases from Mycobacterium leprae; O33076|ML0041|MLCB628.04 (446 aa), FASTA scores: opt: 860, E(): 1.1e-25, (38.65% identity in 538 aa overlap); Q9CD36|ML2528 (475 aa) (475 aa), FASTA scores: opt: 413, E(): 7.1e-09, (37.7% identity in 562 aa overlap). Also similarity with Q99405|PRTM_BACSP M-protease from Bacillus sp. strain KSM-K16 (269 aa), FASTA scores: E(): 7.6e-06, (27.1% identity in 277 aa overlap). And highly similar, but also with gaps, to other mycosins from Mycobacterium tuberculosis e.g. O53945|Rv1796|MTV049.18 (585 aa), FASTA scores: opt: 1173, E(): 2.4e-37, (47.9% identity in 578 aa overlap); the upstream ORF O05461|Rv3883c|MTCY15F10.29 (446 aa) FASTA scores: opt: 910, E(): 1.5e-27, (40.15% identity in 533 aa overlap); O06316|Rv3449|MTCY13E12.02 (455 aa) FASTA scores: opt: 477, E(): 2.7e-11, (38.75% identity in 550 aa overlap); etc. Contains Pro rich protein with two serine protease, subtilase family active site motifs: aspartic acid active site motif (PS00136); and histidine active site motif (PS00137). Belongs to peptidase family S8 (also known as the subtilase family), pyrolysin subfamily. Thought to be cleaved into smaller molecular weight proteins, 36 and 29 KDA (see citation below). O05458 No intermediary metabolism and respiration O05458 O05458 GO:0004252,GO:0006508 3.4.21.- O05458 NC_000962.3 Mycobrowser_v5 CDS 3577033 3580200 . - 0 Rv3202c Rv3202c Has both ATPase and helicase activities Possible ATP-dependent DNA helicase Rv3202c, (MTCY07D11.24, MTV014.46c), len: 1055 aa. Possible ATP-dependent DNA helicase, showing some similarity to UvrD proteins e.g. Q9FCK5|2SC3B6.07 putative ATP-dependent DNA helicase from Streptomyces coelicolor (1159 aa), FASTA scores: opt: 666, E(): 1e-29, (34.5% identity in 1154 aa overlap); Q9L7T3|UVRD|PA5443 mismatch repair protein MUTU (DNA helicase II) from Pseudomonas aeruginosa (728 aa), FASTA scores: opt: 239, E(): 7.3e-06, (23.8% identity in 677 aa overlap) (no similarity in C-terminal part for this one); etc. C-terminal region similar to Q9FDU2|ORF3 ORF3 protein (fragment) from Streptomyces griseus (551 aa), FASTA scores: opt: 800, E(): 1.7e-37, (36.2% identity in 525 aa overlap); and Q9ZG15 hypothetical 35.5 KDA protein from Rhodococcus erythropolis (323 aa), FASTA scores: opt: 232, E(): 9.7e-06, (28.55% identity in 266 aa overlap). O53348 No information pathways O53348 O53348 GO:0004003,GO:0003677,GO:0006281,GO:0005524 3.6.1.- O53348 NC_000962.3 Mycobrowser_v5 CDS 3580638 3581312 . + 0 Rv3203 lipV Function unknown; presumed lipolytic enzyme involved in cellular metabolism. Possible lipase LipV Rv3203, (MTCY07D11.23c), len: 224 aa. Possible lipV, hydrolase lipase, showing some similarity to other lipases e.g. Q9JSN0|NMA2216 putative hydrolase from Neisseria meningitidis (serogroup A) (312 aa), FASTA scores: opt: 192, E(): 0.00016, (45.2% identity in 73 aa overlap); Q9RK95|SCF1.09 putative hydrolase from Streptomyces coelicolor (258 aa), FASTA scores: opt: 188, E(): 0.00024, (30.1% identity in 226 aa overlap); Q9KZC3|SC6F7.19c putative lipase from Streptomyces coelicolor (269 aa), FASTA scores: opt: 179, E(): 0.00086, (36.35% identity in 121 aa overlap); etc. Equivalent to AAK47641 Hydrolase, alpha/beta hydrolase family from Mycobacterium tuberculosis strain CDC1551 (261 aa) but shorter 37 aa. Contains serine active site signature of lipases (PS00120). L0TC47 No intermediary metabolism and respiration O05863 L0TC47 GO:0016787 3.1.-.- L0TC47 NC_000962.3 Mycobrowser_v5 CDS 3581315 3581620 . + 0 Rv3204 Rv3204 Causes methylation Possible DNA-methyltransferase (modification methylase) Rv3204, (MTCY07D11.22c), len: 101 aa. Possible DNA methyltransferase, similar to many hypothetical bacteriel proteins and methyltransferases e.g. Q9KT40|VC1065 methylated-DNA--protein-cysteine methyltransferase-related protein from Vibrio cholerae (100 aa), FASTA scores: opt: 170, E(): 2.8e-05, (34.35% identity in 99 aa overlap); Q9UTN9|SPAC1250.04c putative methyltransferase from Schizosaccharomyces pombe (Fission yeast) (108 aa), FASTA scores: opt: 161, E(): 0.00013, (36.65% identity in 101 aa overlap); Q9YDF4|APE0959 175 AA long hypothetical methylated-DNA--protein-cysteine methyltransferase from Aeropyrum pernix (175 aa), FASTA scores: opt: 144, E(): 0.003, (37.95% identity in 87 aa overlap); Q50855 putative methylguanine-DNA methyltransferase from Myxococcus xanthus (147 aa), FASTA scores: opt: 141, E(): 0.0041, (37.65% identity in 93 aa overlap); etc. O05862 No information pathways O05862 O05862 GO:0008168,GO:0006281 2.1.1.- O05862 NC_000962.3 Mycobrowser_v5 CDS 3585677 3585949 . - 0 Rv3208A TB9.4 Function unknown Conserved protein TB9.4 Rv3208A, len: 90 aa. TB9.4, conserved protein (see citations below), equivalent to Q9CCH1|ML0814 hypothetical protein from Mycobacterium leprae (82 aa), FASTA scores: opt: 411, E(): 1.8e-22, (81.0% identity in 79 aa overlap). Also similar, but shorter in N-terminus, to Q9FBI9|SCP8.32c putative ATP-binding protein from Streptomyces coelicolor (94 aa), FASTA scores: opt: 246, E(): 8.1e-11, (53.4% identity in 73 aa overlap); Q9DGP6 (alias Q9DGP4) glutamate decarboxylase 67 KDA isoform (fragment) from Alepocephalus bairdii (182 aa), FASTA scores: opt: 100, E(): 2.6, (35.3% identity in 85 aa overlap). Corresponds to Statens Serum Institute antigen, CYP10 TB9.4. Has N-terminal sequence, vevkigitdsprelv. Q6MWZ8 No conserved hypotheticals Q6MWZ8 Q6MWZ8 NC_000962.3 Mycobrowser_v5 CDS 3586274 3586834 . + 0 Rv3209 Rv3209 Function unknown Conserved hypothetical threonine and proline rich protein Rv3209, (MTCY07D11.17c), len: 186 aa. Conserved hypothetical thr-, pro-rich protein, equivalent (but shorter 36 aa in N-terminus) to Q9CCH2|ML0813 putative membrane protein from Mycobacterium leprae (195 aa), FASTA scores: opt: 508, E(): 1.4e-15, (58.4% identity in 185 aa overlap). Also some similarity with Q10390|MMS3_MYCTU|MMPS3|Rv2198c|MT2254|MTCY190.09c probable conserved transmembrane transport protein from M. tuberculosis (299 aa), FASTA scores: opt: 339, E(): 3.7e-08, (35.0% identity in 180 aa overlap); and Q9CCE9|MMPS3|ML0877 putative membrane protein from Mycobacterium leprae (293 aa), FASTA scores: opt: 272, E(): 2.8e-05, (36.4% identity in 173 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). O05857 No conserved hypotheticals O05857 O05857 NC_000962.3 Mycobrowser_v5 CDS 3594468 3595433 . + 0 Rv3218 Rv3218 Function unknown Conserved protein Rv3218, (MTCY07D11.08c), len: 321 aa. Conserved protein, similar to several hypothetical bacterial proteins e.g. Q9F3M0|2SC7G11.03c from Streptomyces coelicolor (322 aa), FASTA scores: opt: 694, E(): 4.2e-35, (39.95% identity in 328 aa overlap); Q9A0J4|SPY0752 from Streptomyces pyogenes (340 aa), FASTA scores: opt: 187, E(): 0.00033, (30.5% identity in 141 aa overlap); O31502|YERQ from Bacillus subtilis (303 aa), FASTA scores: opt: 184, E(): 0.00045, (34.15% identity in 126 aa overlap); etc. O05848 No conserved hypotheticals O05848 O05848 GO:0004143,GO:0007205 O05848 NC_000962.3 Mycobrowser_v5 CDS 3622249 3623091 . - 0 Rv3243c Rv3243c Unknown Unknown protein Rv3243c, (MTCY20B11.18c), len: 280 aa. Unknown protein. O05888 No conserved hypotheticals O05888 NC_000962.3 Mycobrowser_v5 CDS 3592254 3593372 . + 0 Rv3215 entC Could be involved in enterobactin biosynthesis. Enterobactin is an iron-chelating compound involved in transporting iron from the bacterial environment into the cell cytoplasm. Could be also involved in 2,3-dihydroxybenzoate or enterochelin or menaquinone biosynthesis [catalytic activity: chorismate = isochorismate]. Probable isochorismate synthase EntC (isochorismate hydroxymutase) (enterochelin biosynthesis) Rv3215, (MTCY07D11.11c), len: 372 aa. Probable entC, isochorismate synthase, equivalent to Q9CCH5|ENTC|ML0808 putative isochorismate synthase from Mycobacterium leprae (577 aa), FASTA scores: opt: 1817, E(): 5.5e-105, (73.5% identity in 366 aa overlap). Also similar to others e.g. Q9F639|MXCD protein involved in myxochelin-type iron chelator biosynthesis (see citation below) from Stigmatella aurantiaca (408 aa), FASTA scores: opt: 893, E(): 6.2e-48, (41.6% identity in 382 aa overlap); P45744|DHBC_BACSU isochorismate synthase from Bacillus subtilis (398 aa), FASTA scores: opt: 883, E(): 2.5e-47, (40.45% identity in 393 aa overlap); Q9KI93|CSBC isochorismate synthase (fragment) from Azotobacter vinelandii (361 aa), FASTA scores: opt: 794, E(): 7.6e-42, (45.65% identity in 298 aa overlap); and the two Escherichia coli proteins AAG54928|ENTC (alias BAB34055|ECS0632) isochorismate hydroxymutase 2 from Escherichia coli strain O157:H7 (391 aa), FASTA scores: opt: 744, E(): 1e-38, (38.8% identity in 340 aa overlap); P10377|ENTC|B0593 isochorismate synthase from Escherichia coli strain K12 (391 aa), FASTA scores: opt: 744, E(): 1e-38, (38.8% identity in 340 aa overlap); etc. Stronger similarity to Escherichia coli entC. Also similar to MTCY253.35. P9WFW9 No intermediary metabolism and respiration O05851 P9WFW9 GO:0008909,GO:0009058 5.4.4.2 P9WFW9 NC_000962.3 Mycobrowser_v5 CDS 3593804 3594235 . - 0 Rv3217c Rv3217c Unknown Probable conserved integral membrane protein Rv3217c, (MTCY07D11.09), len: 143 aa. Probable conserved integral membrane protein, equivalent (highly similar but shorter 30 aa) to Q9CCH6|ML0806 putative membrane protein from Mycobacterium leprae (173 aa). Also similar to others e.g. Q9F3L9|2SC7G11.04 putative integral membrane protein from Streptomyces coelicolor (152 aa), FASTA scores: opt: 177, E(): 0.00024, (33.8% identity in 136 aa overlap). And shows similarity to O34238|MVIN|VC0680 virulence factor MVIN homolog from Vibrio (525 aa), FASTA scores: opt: 126, E(): 0.97, (30.9% identity in 68 aa overlap). First GTG taken. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O05849 No cell wall and cell processes O05849 NC_000962.3 Mycobrowser_v5 CDS 3597551 3597766 . - 0 Rv3221c TB7.3 Function unknown Biotinylated protein TB7.3 Rv3221c, (MTCY07D11.05), len: 71 aa. TB7.3, Biotinylated protein (see citations below), equivalent (appears to have one additional residue) to Q9CCH9|ML0802|BTB7_MYCLE biotinylated protein TB7.3 homolog from Mycobacterium leprae (70 aa), FASTA scores: opt: 367, E(): 4e-18, (90.0% identity in 70 aa overlap); Q9XCD6|BTB7_MYCSM biotinylated protein TB7.3 homolog from Mycobacterium smegmatis (70 aa), FASTA scores: opt: 341, E(): 2.1e-16, (84.05% identity in 69 aa overlap). Similar to C-terminal part of various proteins e.g. Q9HPP8|ACC|VNG1532G biotin carboxylase from Halobacterium sp. strain NRC-1 (610 aa), FASTA scores: opt: 212, E(): 4e-07, (50.0% identity in 68 aa overlap); Q58628|PYCB_METJA|MJ1231 pyruvate carboxylase subunit B from Methanococcus jannaschii (567 aa), FASTA scores: opt: 192, E(): 7.8e-06, (44.8% identity in 58 aa overlap); Q9ZAA7|GCDC glutaconyl-CoA decarboxylase gamma subunit from Acidaminococcus fermentans (145 aa), FASTA scores: opt: 184, E(): 8.9e-06, (39.4% identity in 66 aa overlap); etc. P9WPQ1 No lipid metabolism P0A510 P9WPQ1 P9WPQ1 NC_000962.3 Mycobrowser_v5 CDS 3613664 3614398 . - 0 Rv3238c Rv3238c Unknown Probable conserved integral membrane protein Rv3238c, (MTCY20B11.13c), len: 244 aa. Probable conserved integral membrane protein, similar to several hypothetical proteins and transmembrane proteins e.g. Q9UN92|NRM29 multispanning nuclear envelope membrane protein NURIM (fragment) from Homo sapiens (Human) (261 aa), FASTA scores: opt: 281, E(): 3.3e-11, (30.7% identity in 189 aa overlap); Q9VEG9|CG7655 hypothetical protein from Drosophila melanogaster (Fruit fly) (253 aa), FASTA scores: opt: 242, E(): 1.1e-08, (27.7% identity in 242 aa overlap); BAB48937|MLR1600 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (222 aa), FASTA scores: opt: 137, E(): 0.066, (28.1% identity in 185 aa overlap); BAB57936|SAV1774 aesenical pump membrane protein homolog from Staphylococcus aureus subsp. aureus Mu50 (430 aa), FASTA scores: opt: 125, E(): 0.68, (25.7% identity in 144 aa overlap); etc. O05883 No cell wall and cell processes O05883 O05883 NC_000962.3 Mycobrowser_v5 CDS 3617682 3620531 . - 0 Rv3240c secA1 Involved in protein export. Interacts with the SECY/SECE subunits. SECA has a central role in coupling the hydrolysis of ATP to the transfer of PRE-secretory periplasmic and outer membrane proteins across the membrane. Probable preprotein translocase SecA1 1 subunit Rv3240c, (MTCY20B11.15c), len: 949 aa. Probable secA1, preprotein translocase subunit, component of secretion apparatus (see citations below), highly similar to many e.g. P57996|SEA1_MYCLE from Mycobacterium leprae (940 aa), FASTA scores: opt: 5044, E(): 0, (87.5% identity in 849 aa overlap); P95759|SECA_STRGR from Streptomyces griseus (940 aa), FASTA scores: opt: 2612, E(): 1.9e-134, (61.35% identity in 960 aa overlap); P28366|SECA_BACSU|div+ from Bacillus subtilis (841 aa), FASTA scores: opt: 1776, E(): 4.9e-89, (48.05% identity in 837 aa overlap); etc. Belongs to the SecA family. Part of the prokaryotic protein translocation apparatus which comprise SECA, SECD|Rv2587c, SECE|Rv0638, SECF|Rv2586c, SECG|Rv1440 and SECY|Rv0732. Note that previously known as secA. Binds ATP. P9WGP5 No cell wall and cell processes 1NKT,1NL3 P0A5Y8 P9WGP5 GO:0006605,GO:0005737,GO:0065002,GO:0005886,GO:0005515,GO:0017038,GO:0005524 P9WGP5 NC_000962.3 Mycobrowser_v5 CDS 3596029 3597534 . - 0 Rv3220c Rv3220c Sensor part of a two component regulatory system. Probable two component sensor kinase Rv3220c, (MTCY07D11.06), len: 501 aa. Probable sensor (probably histidine kinase), equivalent to Q9CCH8|ML0803 putative two-component system sensor kinase from Mycobacterium leprae (500 aa). Similar to others e.g. Q9F3M1|2SC7G11.01 putative histidine kinase (fragment) from Streptomyces coelicolor (372 aa), FASTA scores: opt: 1038, E(): 7.4e-56, (48.95% identity in 380 aa overlap); Q9A3K5|CC3198 sensor histidine kinase from Caulobacter crescentus (327 aa), FASTA scores: opt: 311, E(): 1.2e-11, (33.35% identity in 201 aa overlap) (similarity only in C-terminal part for this one); Q9A2T2|CC3474 putative sensor histidine kinase from Caulobacter crescentus (547 aa); etc. C-terminal half shows similarity to many sensor proteins, that respond to various stimuli from Methanobacterium thermoautotrophicum e.g. O26568|MTH468 sensory transduction histidine kinase (554 aa), FASTA scores: opt: 425, E(): 2.1e-18, (34.0% identity in 244 aa overlap); O26546|MTH446 sensory transduction regulatory protein (583 aa), FASTA scores: opt: 380, E(): 1.2e-15, (37.15% identity in 202 aa overlap); O26913|MTH823 sensory transduction regulatory protein (677 aa), FASTA scores: opt: 375, E(): 2.7e-15, (35.4% identity in 195 aa overlap); etc. Seems similar to other prokaryotic sensory transduction histidine kinases. P9WGL5 No regulatory proteins O05846 P9WGL5 GO:0018106,GO:0007165,GO:0000155,GO:0000160,GO:0005524 2.7.13.3 P9WGL5 NC_000962.3 Mycobrowser_v5 CDS 3601016 3602440 . - 0 Rv3225c Rv3225c Acetylation, phosphorylation, substrates unknown GCN5-related N-acetyltransferase, phosphorylase Rv3225c, (MTCY07D11.01), len: 474 aa. Conserved hypothetical protein has GNAT (Gcn5-related N-acetyltransferase) domain in N-terminal part (see Vetting et al. 2005) and phosphotransferase domain in C-terminal part. C-terminal part shows similarity to various bacterial phosphotransferases e.g. BAB49093|MLL1809 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (298 aa), FASTA scores: opt: 557, E(): 2.8e-26, (34.55% identity in 295 aa overlap); P14509|KKA8_ECOLI|APHA aminoglycoside 3'-phosphotransferase from Escherichia coli (271 aa), FASTA scores: opt: 194, E(): 0.00018, (27.75% identity in 227 aa overlap); Q53826|CPH capreomycin phosphotransferase from Streptomyces capreolus (281 aa), FASTA scores: opt: 178, E(): 0.0017, (30.5% identity in 269 aa overlap). O05841 No intermediary metabolism and respiration O05841 O05841 GO:0008152,GO:0008080 2.-.-.- O05841 NC_000962.3 Mycobrowser_v5 CDS 3638811 3639302 . - 0 Rv3258c Rv3258c Function unknown Conserved hypothetical protein Rv3258c, (MTV015.03c), len: 163 aa. Conserved hypothetical protein, equivalent to Q9CCJ8|ML0762 hypothetical protein from Mycobacterium leprae (165 aa), FASTA scores: opt: 840, E(): 9.9e-42, (76.9% identity in 169 aa overlap). Also similar to Q9KZL4|SCE34.11c hypothetical 15.0 KDA protein from Streptomyces coelicolor (140 aa), FASTA scores: opt: 353, E(): 1.1e-13, (48.3% identity in 147 aa overlap); and shows really weak similarity to other bacterial proteins. O53351 No conserved hypotheticals O53351 O53351 O53351 NC_000962.3 Mycobrowser_v5 CDS 3602564 3603322 . - 0 Rv3226c Rv3226c Function unknown Conserved hypothetical protein Rv3226c, (MTCY20B11.01c), len: 252 aa. Conserved hypothetical protein, similar to various hypothetical bacterial proteins e.g. Q9CCI2|ML0793 putative bacteriophage protein from Mycobacterium leprae (252 aa), FASTA scores: opt: 1183, E(): 3.8e-68, (70.65% identity in 252 aa overlap); BAB54183|MLR7795 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (369 aa), FASTA scores: opt: 417, E(): 2.9e-19, (33.75% identity in 252 aa overlap); O64131 YOQW protein from Bacteriophage SPBc2 (224 aa), FASTA scores: opt: 413, E(): 3.4e-19, (38.5% identity in 244 aa overlap); O31916 YOQW protein from Bacillus subtilis (224 aa), FASTA scores: opt: 413, E(): 3.4e-19, (38.5% identity in 244 aa overlap); O34906 YOAM protein from Bacillus subtilis (227 aa), FASTA scores: opt: 401, E(): 2e-18, (37.7% identity in 244 aa overlap); Q9K4A5|SC7E4.11 hypothetical 30.8 KDA protein from Streptomyces coelicolor (271 aa), FASTA scores: opt: 383, E(): 3.3e-17, (39.6% identity in 283 aa overlap); etc. O05872 No conserved hypotheticals O05872 O05872 O05872 NC_000962.3 Mycobrowser_v5 CDS 3603377 3604729 . + 0 Rv3227 aroA Involved in the biosynthesis of chorismate within the biosynthesis of aromatic amino acids (the shikimate pathway). Acts in the sixth step of this pathway. [catalytic activity: phosphoenolpyruvate + 3-phosphoshikimate = orthophosphate + O(5)-(1-carboxyvinyl)-3-phosphoshikimate]. 3-phosphoshikimate 1-carboxyvinyltransferase AroA (5-enolpyruvylshikimate-3-phosphate synthase) (EPSP synthase) (EPSPS) Rv3227, (MTCY20B11.02), len: 450 aa. AroA, 3-phosphoshikimate 1-carboxyvinyl transferase (see citation below), equivalent (but C-terminus longer) to Q9CCI3|AROA|ML0792 putative 3-phosphoshikimate 1-carboxyvinyl transferase from Mycobacterium leprae (430 aa), FASTA scores: opt: 1466, E(): 1.4e-78, (55.05% identity in 427 aa overlap). Contains PS00885 EPSP synthase signature 2. Belongs to the EPSP synthase family. P9WPY5 No intermediary metabolism and respiration 2BJB,2O0B,2O0D,2O0E,2O0X,2O0Z,2O15 P22487 P9WPY5 GO:0009073,GO:0005737,GO:0003866 2.5.1.19 P9WPY5 NC_000962.3 Mycobrowser_v5 CDS 3604726 3605718 . + 0 Rv3228 Rv3228 Function unknown Conserved hypothetical protein Rv3228, (MTCY20B11.03), len: 330 aa. Conserved hypothetical protein, equivalent to Q9CCI4|ML0791 hypothetical protein from Mycobacterium leprae (327 aa), FASTA scores: opt: 1828, E(): 1e-98, (84.0% identity in 331 aa overlap). Also similar to several hypothetical bacterial proteins e.g. Q9K4A8|SC7E4.08c from Streptomyces coelicolor (337 aa), FASTA scores: opt: 1051, E(): 1e-53, (52.65% identity in 338 aa overlap); Q9HUL3|PA4952 from Pseudomonas aeruginosa (339 aa), FASTA scores: opt: 392 ,E(): 1.4e-15, (34.85% identity in 281 aa overlap); Q9PFV1|XF0556 from Xylella fastidiosa (341 aa), FASTA scores: opt: 367, E(): 4e-14, (36.85% identity in 247 aa overlap); P45339|YJEQ_HAEIN|HI1714 from Haemophilus influenzae (346 aa), FASTA scores: opt: 355, E(): 2e-13, (31.65% identity in 281 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A. O05873 No conserved hypotheticals O05873 O05873 GO:0003924,GO:0046872,GO:0005525 O05873 NC_000962.3 Mycobrowser_v5 CDS 3608364 3608873 . - 0 Rv3231c Rv3231c Function unknown Conserved protein Rv3231c, (MTCY20B11.06c), len: 169 aa. Conserved protein, similar to Q9KYX9|SCE33.03c hypothetical 17.4 KDA protein from Streptomyces coelicolor (167 aa), FASTA scores: opt: 415, E(): 6.6e-19, (49.1% identity in 171 aa overlap). O05876 No conserved hypotheticals O05876 O05876 NC_000962.3 Mycobrowser_v5 CDS 3608870 3609757 . - 0 Rv3232c ppk2 Catalyzes the reversible transfer of phosphate from polyphosphate (polyp) to form GTP [catalytic activity: GDP + {phosphate}(N) = GTP + {phosphate}(N-1)]. Polyphosphate kinase Ppk2 (polyphosphoric acid kinase) Rv3232c, (MTCY20B11.07c), len: 295 aa (start uncertain). Ppk2, polyphosphate kinase 2, highly similar to Q9I154|PA2428 hypothetical protein from Pseudomonas aeruginosa (304 aa), FASTA scores: opt: 1057, E(): 6.8e-62, (60.7% identity in 252 aa overlap); Q9I6Z1|PA0141 hypothetical protein from Pseudomonas aeruginosa (298 aa), FASTA scores: opt: 990, E(): 1.6e-57, (54.6% identity in 249 aa overlap); and other hypothetical bacterial proteins. Note that previously known as pvdS. Ppk2|Rv3232c and NdkA|Rv2445c interact (See Sureka et al., 2009). O05877 No intermediary metabolism and respiration O05877 O05877 O05877 NC_000962.3 Mycobrowser_v5 CDS 3610374 3611189 . - 0 Rv3234c tgs3 May be involved in synthesis of triacylglycerol Putative triacylglycerol synthase (diacylglycerol acyltransferase) Tgs3 Rv3234c, (MTCY20B11.09c), len: 271 aa. Putative tgs3, triacylglycerol synthase (See Daniel et al., 2004), similar to C-terminus of Mycobacterium tuberculosis hypothetical proteins e.g. P71694|Rv1425|MTCY21B4.43|MTCY493.29c (459 aa), FASTA scores: opt: 498, E(): 5.2e-24, (36.8% identity in 261 aa overlap); MTCY03A2.28; MTCY31.23; MTCY493_29; MTCY28_26; MTV013_8; MTY13E12_33; etc. Also similar to Q9X7A8|MLCB1610.05|ML1244 conserved membrane protein from Mycobacterium leprae (491 aa), FASTA scores: opt: 309, E(): 4.3e-12, (33.35% identity in 189 aa overlap). P9WKC5 No lipid metabolism O05879 P9WKC5 GO:0006071,GO:0008610,GO:0004144 2.3.1.20 P9WKC5 NC_000962.3 Mycobrowser_v5 CDS 3633675 3635063 . + 0 Rv3254 Rv3254 Function unknown Conserved hypothetical protein Rv3254, (MTCY20B11.29), len: 462 aa. Conserved hypothetical protein, similar to CAC37877|SC1G7.02 putative secreted protein from Streptomyces coelicolor (440 aa), FASTA scores: opt: 606, E(): 6.2e-31, (31.7% identity in 445 aa overlap); O86550|SC1F2.13c hypothetical 50.7 KDA protein from Streptomyces coelicolor (476 aa), FASTA scores: opt: 577, E(): 4.5e-29, (32.5% identity in 400 aa overlap); Q9L0A8|SCC24.09 putative secreted protein from Streptomyces coelicolor (468 aa), FASTA scores: opt: 380, E(): 1.3e-16, (30.7% identity in 391 aa overlap); BAB48792|MLL1411 probable FAD-dependent monooxygenase from Rhizobium loti (Mesorhizobium loti) (421 aa), FASTA scores: opt: 128, E(): 1.1, (25.2% identity in 397 aa overlap); Q9L7X9|BENF benzoate-specific porin-like protein from Pseudomonas putida (397 aa), FASTA scores: opt: 119, E(): 4, (24.85% identity in 157 aa overlap); etc. Also similar to N-terminus of AAK46259|MT1987 putative ferredoxin reductase, electron transfer component from Mycobacterium tuberculosis strain CDC1551 (839 aa), FASTA scores: opt: 493, E(): 1.5e-23, (30.65% identity in 382 aa overlap). O05897 No conserved hypotheticals O05897 NC_000962.3 Mycobrowser_v5 CDS 3826548 3826958 . + 0 Rv3408 vapC47 Unknown Possible toxin VapC47. Contains PIN domain. Rv3408, (MTCY78.20c), len: 136 aa. Possible vapC47, toxin, part of toxin-antitoxin (TA) operon with Rv3407, contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. O50411|Rv3384c|MTV004.42c (130 aa), FASTA scores: opt: 243, E(): 1.7e-09, (35.1% identity in 131 aa overlap); P95252|Rv1962c|MTCY09F9.02 (135 aa), FASTA scores: opt: 191, E(): 5e-06, (35.5% identity in 138 aa overlap), etc. P9WF49 No virulence, detoxification, adaptation Q50717 P9WF49 P9WF49 NC_000962.3 Mycobrowser_v5 CDS 3613121 3613603 . - 0 Rv3237c Rv3237c Function unknown Conserved protein Rv3237c, (MTCY20B11.12c), len: 160 aa. Conserved protein, equivalent to Q9CCI6|ML0781 hypothetical protein from Mycobacterium leprae (160 aa), FASTA scores: opt: 828, E(): 1.5e-45, (80.6% identity in 160 aa overlap); and similar to other hypothetical bacterial proteins and more weakly to putative potassium channels e.g. Q9RV81|DR1148 conserved hypothetical protein from Deinococcus radiodurans (175 aa), FASTA scores: opt: 420, E(): 9.5e-20, (37.95% identity in 158 aa overlap); O69959|SC4H2.04c hypothetical 17.1 KDA protein from Streptomyces coelicolor (161 aa), FASTA scores: opt: 315, E(): 3.8e-13, (40.0% identity in 150 aa overlap); Q9HNH3|PCHB|VNG2104G potassium channel homolog from Halobacterium sp. strain NRC-1 (418 aa), FASTA scores: opt: 158, E(): 0.007, (31.45% identity in 124 aa overlap); Q58752|YD57_METJA|MJ1357 putative potassium channel protein from Methanococcus jannaschii (343 aa), FASTA scores: opt: 143, E(): 0.053, (33.8% identity in 68 aa overlap). O05882 No conserved hypotheticals O05882 O05882 GO:0003824,GO:0008324,GO:0008152,GO:0005886,GO:0006813 O05882 NC_000962.3 Mycobrowser_v5 CDS 3652679 3653347 . - 0 Rv3271c Rv3271c Unknown Probable conserved integral membrane protein Rv3271c, (MTCY71.11c), len: 222 aa. Probable conserved integral membrane protein, similar to others e.g. Q9RD35|SCM1.07c from Streptomyces coelicolor (230 aa), FASTA scores: opt: 360, E(): 4.7e-16, (33.85% identity in 195 aa overlap); Q9X897|SCE2.02c from Streptomyces coelicolor (234 aa), FASTA scores: opt: 357, E(): 7.3e-16, (33.85% identity in 195 aa overlap); Q9D0E0 2610024A01RIK protein from Mus musculus (Mouse) (288 aa), FASTA scores: opt: 191, E(): 3.7e-05, (23.65% identity in 207 aa overlap). P96876 No cell wall and cell processes P96876 P96876 GO:0006812,GO:0016020,GO:0055085,GO:0008324 NC_000962.3 Mycobrowser_v5 CDS 3623159 3624910 . - 0 Rv3244c lpqB Unknown Probable conserved lipoprotein LpqB Rv3244c, (MTCY20B11.19c), len: 583 aa. Probable lpqB, conserved lipoprotein; contains appropriately placed lipoprotein signature (PS00013). Equivalent to Q9CCJ0|LPQB|ML0775 putative lipoprotein from Mycobacterium leprae (589 aa), FASTA scores: opt: 3375, E(): 1.4e-186, (87.9% identity in 579 aa overlap). Also similar to various proteins (in particular transferases) e.g. Q9KYX0|SCE33.13c putative lipoprotein from Streptomyces coelicolor (615 aa), FASTA scores: opt: 228, E(): 1.3e-05, (25.5% identity in 624 aa overlap); O87992|BBLPS1.19c putative glutamine amidotransferase from Bordetella bronchiseptica (Alcaligenes bronchisepticus) (628 aa), FASTA scores: opt: 162, E(): 0.079, (28.05% identity in 171 aa overlap); Q9L2F4|SC7A8.01 putative sugar kinase (fragment) from Streptomyces coelicolor (434 aa), FASTA scores: opt: 143, E(): 0.72, (27.65% identity in 293 aa overlap); etc. P9WK37 No cell wall and cell processes O05889 P9WK37 GO:0005886 P9WK37 NC_000962.3 Mycobrowser_v5 CDS 3627419 3628063 . - 0 Rv3247c tmk Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis [catalytic activity: ATP + thymidine 5'-phosphate = ADP + thymidine 5'-diphosphate]. Thymidylate kinase Tmk (dTMP kinase) (thymidylic acid kinase) (TMPK) Rv3247c, (MTCY20B11.22c), len: 214 aa. tmk, thymidylate kinase, equivalent to Q9CCJ3|TMK|ML0772 putative thymidylate kinase from Mycobacterium leprae (210 aa), FASTA scores: opt: 1023, E(): 4.8e-57, (77.3% identity in 207 aa overlap). Also similar to other thymidylate kinases e.g. Q9RQJ9|KTHY_CAUCR|TMK|CC1824 from Caulobacter crescentus (208 aa), FASTA scores: opt: 179, E(): 0.0003, (31.3% identity in 214 aa overlap); Q9V1E9|KTHY_PYRAB|TMK|PAB0319 from Pyrococcus abyssi (205 aa), FASTA scores: opt: 176, E(): 0.00045, (29.1% identity in 189 aa overlap); etc. Belongs to the thymidylate kinase family. P9WKE1 No intermediary metabolism and respiration 1G3U,1GSI,1GTV,1MRN,1MRS,1N5I,1N5J,1N5K,1N5L,1W2G,1W2H O05891 P9WKE1 GO:0006233,GO:0006235,GO:0046872,GO:0004798,GO:0005524 2.7.4.9 P9WKE1 NC_000962.3 Mycobrowser_v5 CDS 3636275 3637315 . - 0 Rv3256c Rv3256c Function unknown Conserved protein Rv3256c, (MTV015.01c-MTCY20B11.31c), len: 346 aa. Conserved protein, equivalent to Q9CCJ6|ML0764 hypothetical protein from Mycobacterium leprae (365 aa), FASTA scores: opt: 1574, E(): 1.4e-82, (75.35% identity in 365 aa overlap). Also similar to other hypothetical bacterial proteins e.g. Q9KZL8|SCE34.07c from Streptomyces coelicolor (375 aa), FASTA scores: opt: 171, E(): 0.012, (31.1% identity in 376 aa overlap); P55709|Y4YA_RHISN from Rhizobium sp. strain NGR234 (457 aa), FASTA scores: opt: 140, E(): 0.84, (28.75% identity in 233 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O05899 No conserved hypotheticals O05899 O05899 NC_000962.3 Mycobrowser_v5 CDS 3773574 3774155 . - 0 Rv3362c Rv3362c Function unknown Probable ATP/GTP-binding protein Rv3362c, (MTV004.19c), len: 193 aa. Probable ATP/GTP-binding protein, similar to others from Streptomyces coelicolor e.g. O86519|SC1C2.18c (174 aa), FASTA scores: opt: 731, E(): 9.8e-41, (66.85% identity in 169 aa overlap); Q9XAE1|SC6G9.41c (191 aa), FASTA scores: opt: 730, E(): 1.2e-40, (63.55% identity in 173 aa overlap); Q9L235|SC1A2.06 (184 aa), FASTA scores: opt: 650, E(): 1.9e-35, (55.95% identity in 177 aa overlap); Q9RJ74|SCI41.10c (176 aa), FASTA scores: opt: 618, E(): 2.3e-33, (55.9% identity in 161 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). O50391 No conserved hypotheticals O50391 O50391 GO:0000166 O50391 NC_000962.3 Mycobrowser_v5 CDS 3620610 3621254 . - 0 Rv3241c Rv3241c Function unknown, but may be involved in transduction mechanism Conserved protein Rv3241c, (MTCY20B11.16c), len: 214 aa. Conserved protein, similar to many hypothetical proteins and to some putative ribosomal proteins e.g. Q9CCI7|ML0778 hypothetical protein from Mycobacterium leprae (229 aa), FASTA scores: opt: 1234, E(): 1.3e-72, (89.3% identity in 206 aa overlap); Q9KYX2|SCE33.11c hypothetical 27.9 KDA protein from Streptomyces coelicolor (254 aa), FASTA scores: opt: 487, E(): 2.2e-24, (47.6% identity in 210 aa overlap); Q9FLV3 protein similar to ribosomal protein 30S subunit from Arabidopsis thaliana (Mouse-ear cress) (365 aa), FASTA scores: opt: 264, E(): 7e-10, (26.4% identity in 212 aa overlap); P19954|RR30_SPIOL|RPS22 plastid-specific 30S ribosomal protein 1, chloroplast, from Spinacia oleracea (Spinach) (302 aa), FASTA scores: opt: 261, E(): 9.3e-10, (26.15% identity in 214 aa overlap); P47995|YSEA_STACA hypothetical protein in SECA 5'region (ORF1) (fragment) (belongs to the S30AE family of ribosomal proteins) from Staphylococcus carnosus (165 aa), FASTA scores: opt: 201, E(): 4.2e-06, (33.35% identity in 147 aa overlap); etc. O05886 No information pathways O05886 O05886 GO:0044238 O05886 NC_000962.3 Mycobrowser_v5 CDS 3621570 3622211 . - 0 Rv3242c Rv3242c Function unknown Conserved hypothetical protein Rv3242c, (MTCY20B11.17c), len: 213 aa. Conserved hypothetical protein, highly similar in N-terminus to Q9CCI9|ML0776 hypothetical protein from Mycobacterium leprae (85 aa), FASTA scores: opt: 324, E(): 1.7e-13, (78.1% identity in 64 aa overlap). Also similar to Q9RUJ7|DR1389 putative competence protein COMF from Deinococcus radiodurans (219 aa), FASTA scores: opt: 223, E(): 6.3e-07, (35.8% identity in 215 aa overlap); BAB50338|MLL3453 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (240 aa), FASTA scores: opt: 218, E(): 1.4e-06, (28.5% identity in 224 aa overlap); Q9A9Y1|CC0830 competence protein F from Caulobacter crescentus (265 aa), FASTA scores: opt: 182, E(): 0.00026, (30.15% identity in 219 aa overlap); etc. Equivalent to AAK47682 from Mycobacterium tuberculosis strain CDC1551 (241 aa) but shorter 29 aa. Contains purine/pyrimidine phosphoribosyl transferases signature (PS00103). Seems to belong to purine/pyrimidine phosphoribosyl transferase family. O05887 No conserved hypotheticals O05887 O05887 GO:0016740,GO:0009116 O05887 NC_000962.3 Mycobrowser_v5 CDS 3630571 3630738 . - 0 Rv3251c rubA Involved in the hydrocarbon hydroxylating system, which transfers electrons from NADH to rubredoxin reductase and then through rubredoxin to alkane 1 monooxygenase. Probable rubredoxin RubA Rv3251c, (MTCY20B11.26c), len: 55 aa. Probable rubA, rubredoxin, highly similar to other rubredoxins (but sometimes shorter) e.g. Q9AE67|RUBA3 from Rhodococcus erythropolis (61 aa), FASTA scores: opt: 335, E(): 1e-17, (73.6% identity in 53 aa overlap); P00272|RUB2_PSEOL|ALKG from Pseudomonas oleovorans (172 aa), FASTA scores: opt: 278, E(): 2.7e-13, (65.3% identity in 49 aa overlap); CAC38028|ALKG from Alcanivorax borkumensis (174 aa), FASTA scores: opt: 271, E(): 8.6e-13, (62.0% identity in 50 aa overlap); Q9WWW4|ALKG from Pseudomonas putida (175 aa), FASTA scores: opt: 270, E(): 1e-12, (61.8% identity in 55 aa overlap); etc. Also highly similar to C-terminus of Q9XBM1|ALKB alkane 1-monooxygenase from Prauserella rugosa (490 aa), FASTA scores: opt: 296, E(): 2.9e-14, (75.5% identity in 49 aa overlap). Also similar to neighbouring ORF Mycobacterium tuberculosis rubB (MTCY20B11.25c). Contains rubredoxin signature (PS00202). Belongs to the rubredoxin family. O05894 No intermediary metabolism and respiration O05894 O05894 GO:0022900,GO:0046872,GO:0006810,GO:0009055 O05894 NC_000962.3 Mycobrowser_v5 CDS 3880286 3880399 . - 0 Rv3461c rpmJ Involved in translation mechanism. 50S ribosomal protein L36 RpmJ Rv3461c, (MTCY13E12.14c), len: 37 aa. rpmJ, 50S ribosomal protein L36, equivalent to P45810|RL36_MYCBO|RPMJ from Mycobacterium bovis (37 aa); and Q9X7A2|RL36_MYCLE|RPMJ|ML1961|MLCB1222.31c 50S ribosomal protein L36 from Mycobacterium leprae (37 aa), FASTA scores: opt: 241, E(): 9.7e-14, (86.5% identity in 37 aa overlap). Also highly similar to others e.g. O86772|RL36_STRCO|SC6G4.04 from Streptomyces coelicolor (37 aa), FASTA scores: opt: 233, E(): 4.5e-13, (83.8% identity in 37 aa overlap); P07841|RL36_BACST|RPMJ from Bacillus stearothermophilus (37 aa), FASTA scores: opt: 214, E(): 1.6e-11, (72.95% identity in 37 aa overlap); P12230|RK36_SPIOL|RPL36 from Spinacia oleracea (Spinach) (37 aa), FASTA scores: opt: 211, E(): 2.9e-11, (70.25% identity in 37 aa overlap); etc. Contains PS00828 Ribosomal protein L36 signature. Belongs to the L36P family of ribosomal proteins. P9WH89 No information pathways P0A5W6 P9WH89 GO:0003735,GO:0006412,GO:0005840 P9WH89 NC_000962.3 Mycobrowser_v5 CDS 3629752 3630387 . - 0 Rv3249c Rv3249c Probably involved in a transcriptional mechanism Possible transcriptional regulatory protein (probably TetR-family) Rv3249c, (MTCY20B11.24c), len: 211 aa. Possible transcriptional regulatory protein, TetR family, with similarity to several e.g. Q9AE61|ALKB1 putative TetR-regulatory from Rhodococcus erythropolis (208 aa), FASTA scores: opt: 503, E(): 7.7e-26, (40.6% identity in 192 aa overlap); CAC37620 putative TetR-regulatory protein from Prauserella rugosa (212 aa), FASTA scores: opt: 246, E(): 4.4e-09, (27.95% identity in 186 aa overlap); Q9K4B0|SC7E4.06 putative TetR-family transcriptional from Streptomyces coelicolor (203 aa), FASTA scores: opt: 224, E(): 1.1e-07, (34.5% identity in 197 aa overlap); Q11063|YC55_MYCTU|Rv1255c|MT1294|MTCY50.27 hypothetical transcriptional regulator from Mycobacterium tuberculosis (202 aa), FASTA scores: opt: 191, E(): 1.6e-05, (28.35% identity in 180 aa overlap); etc. Equivalent to AAK47689 from Mycobacterium tuberculosis strain CDC1551 (230 aa) but shorter 19 aa. Could belong to the TetR/AcrR family of transcriptional regulators. Possible helix-turn helix motif at aa 44-65 (+6.66 SD). O05892 No regulatory proteins O05892 O05892 GO:0006350,GO:0003700,GO:0006355 O05892 NC_000962.3 Mycobrowser_v5 CDS 3754293 3755033 . - 0 Rv3349c Rv3349c Possibly involved in the transposition of the insertion sequence IS1561'. Probable transposase Rv3349c, (MTV004.05c), len: 246 aa. Probable transposase pseudogene fragment, similar to part of Q50911|U10634 IS204 putative transposase from nocardia asteroides (377 aa), FASTA scores: opt: 288, E(): 8.3e-11, (48.5% identity in 97 aa overlap); and others. V5QQS8 No insertion seqs and phages Q93IG7 V5QQS8 GO:0004803,GO:0006313,GO:0003677 NC_000962.3 Mycobrowser_v5 CDS 3639872 3640141 . - 0 Rv3260c whiB2 Involved in transcriptional mechanism. Probable transcriptional regulatory protein WhiB-like WhiB2 Rv3260c, (MTV015.05c), len: 89 aa. Probable whiB2 (alternate gene name: whmD), WhiB-like regulatory protein (see Hutter & Dick 1999), similar to WhiB paralogue of Streptomyces coelicolor, wblE gene product (85 aa). Equivalent to Q9CCK0|WHIB2|ML0760 putative transcriptional regulator from Mycobacterium leprae (89 aa), FASTA scores: opt: 550, E(): 6.1e-31, (85.4% identity in 89 aa overlap). Also similar to others e.g. Q9S426 WHMD regulatory protein (see Gomez & Bishai 2000) from Mycobacterium smegmatis (129 aa), FASTA scores: opt: 488, E(): 1.4e-26, (83.55% identity in 85 aa overlap); Q06387|WHIB-STV WHIB-STV protein from Streptomyces griseocarneus (87 aa), FASTA scores: opt: 443, E(): 1.2e-23, (74.7% identity in 83 aa overlap); Q05429|WHIB|WHIB1 transcription-like factor WhiB from Streptomyces aureofaciens (87 aa), FASTA scores: opt: 442, E(): 1.3e-23, (74.7% identity in 83 aa overlap); etc. Equivalent to AAK47700 WhiB-related protein from Mycobacterium tuberculosis strain CDC1551 (123 aa) but shorter 34 aa. Also similar to other Mycobacterium tuberculosis proteins: MTCY07D11.07c (45.1% identity in 71 aa overlap) and MTCY78.13c (37.4% identity in 91 aa overlap). Start chosen by homology but ORF continues to ATG upstream at 3754. O53353 No regulatory proteins O53353 O53353 O53353 NC_000962.3 Mycobrowser_v5 CDS 3630384 3630566 . - 0 Rv3250c rubB Involved in the hydrocarbon hydroxylating system to convert conversion of dodecane to lauric acid, which transfers electrons from NADH to rubredoxin reductase and then through rubredoxin to alkane 1 monooxygenase. Probable rubredoxin RubB Rv3250c, (MTCY20B11.25c), len: 60 aa. Probable rubB, rubredoxin, highly similar to other rubredoxins e.g. Q9AE66|RUBA4 from Rhodococcus erythropolis (60 aa), FASTA scores: opt: 391, E(): 2.2e-21, (83.05% identity in 59 aa overlap); Q9AE63|RUBA2 from Rhodococcus erythropolis (63 aa), FASTA scores: opt: 380, E(): 1.4e-20, (83.9% identity in 56 aa overlap); P42453|RUBR_ACICA|RUBA from Acinetobacter calcoaceticus (54 aa), FASTA scores: opt: 315, E(): 4.9e-16, (69.8% identity in 53 aa overlap); Q9HTK7|PA5351 from Pseudomonas aeruginosa (55 aa), FASTA scores: opt: 298, E(): 8e-15, (64.15% identity in 53 aa overlap); Q9PGC3|XF0379 from Xylella fastidiosa (57 aa), FASTA scores: opt: 263, E(): 2.5e-12, (59.25% identity in 54 aa overlap); etc. Also similar to neighbouring ORF M. tuberculosis RubA (MTCY20B11.26c). Contains rubredoxin signature (PS00202). Belongs to the rubredoxin family. I6YFL7 No intermediary metabolism and respiration 2KN9 O05893 I6YFL7 GO:0022900,GO:0046872,GO:0006810,GO:0009055 I6YFL7 NC_000962.3 Mycobrowser_v5 CDS 3632097 3633584 . - 0 Rv3253c Rv3253c Thought to be involved in cationic amino acid transport across the membrane. Responsible for the translocation of the substrate across the membrane. Possible cationic amino acid transport integral membrane protein Rv3253c, (MTCY20B11.28c), len: 495 aa. Possible cationic amino acid transporter, integral membrane protein, similar to many e.g. O69844|SC1C3.02 putative cationic amino acid transporter from Streptomyces coelicolor (503 aa), FASTA scores: opt: 1649, E(): 5.8e-92, (52.6% identity in 485 aa overlap); Q9AE69 putative transporter (fragment) from Rhodococcus erythropolis (385 aa), FASTA scores: opt: 1594, E(): 9.7e-89, (62.0% identity in 387 aa overlap); Q9PBD7|XF2207 cationic amino acid transporter from Xylella fastidiosa (483 aa), FASTA scores: opt: 1079, E(): 1.2e-57, (40.55% identity in 493 aa overlap); Q9SRU9|F20H23.25 putative cationic amino acid transporter from Arabidopsis thaliana (Mouse-ear cress) (614 aa), FASTA scores: opt: 802, E(): 6.7e-41, (36.4% identity in 445 aa overlap); P30823|CTR1_RAT|SLC7A1|ATRC1 high-affinity cationic amino acid transporter-1 from Rattus norvegicus (Rat) (624 aa), FASTA scores: opt: 782, E(): 1.1e-39, (36.1% identity in 432 aa overlap); etc. Relatives in Mycobacterium tuberculosis include: MTCY3G12.14, E(): 5.6e-31; MTCY39.19, E(): 1.6e-14. Seems to belong to the APC family. O05896 No cell wall and cell processes O05896 O05896 GO:0006865,GO:0016021,GO:0055085,GO:0015171 O05896 NC_000962.3 Mycobrowser_v5 CDS 3639425 3639844 . + 0 Rv3259 Rv3259 Function unknown Conserved hypothetical protein Rv3259, (MTV015.04), len: 139 aa. Conserved hypothetical protein, equivalent, but shorter 29 aa, to Q9CCJ9|ML0761 hypothetical protein from Mycobacterium leprae (167 aa), FASTA scores: opt: 846, E(): 2.2e-47, (89.2% identity in 139 aa overlap). C-terminus highly similar to Q9S425 hypothetical 6.0 KDA protein (fragment) from Mycobacterium smegmatis (54 aa), FASTA scores: opt: 275, E(): 2.7e-11, (81.15% identity in 53 aa overlap). Also similar to Q9KZL3|SCE34.12 from Streptomyces coelicolor (117 aa), FASTA scores: opt: 152, E(): 0.004, (34.15% identity in 126 aa overlap). Equivalent to AAK47699 from Mycobacterium tuberculosis strain CDC1551 (175 aa) but shorter 36 aa. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53352 No conserved hypotheticals O53352 O53352 O53352 NC_000962.3 Mycobrowser_v5 CDS 3637312 3638709 . - 0 Rv3257c pmmA This enzyme converses D-mannose 1-phosphate in D-mannose 6-phosphate [catalytic activity: D-mannose 1-phosphate = D-mannose 6-phosphate]. Probable phosphomannomutase PmmA (PMM) (phosphomannose mutase) Rv3257c, (MTV015.02c), len: 465 aa. Probable pmmA, phosphomannomutase, equivalent to Q9CCJ7|PMMA|ML0763 phosphomannomutase from Mycobacterium leprae (468 aa), FASTA scores: opt: 2533, E(): 2e-145, (83.1% identity in 468 aa overlap). Also similar to many e.g. Q9KZL6|MANB from Streptomyces coelicolor (454 aa), FASTA scores: opt: 1820, E(): 2e-102, (63.2% identity in 459 aa overlap); Q9PGN8|XF0260 from Xylella fastidiosa (500 aa), FASTA scores: opt: 1085, E(): 4.7e-58, (40.7% identity in 462 aa overlap); Q9EY19|MANB from Salmonella enterica subsp. arizonae (456 aa), FASTA scores: opt: 988, E(): 3.1e-52, (38.65% identity in 445 aa overlap); etc. Belongs to the phosphohexose mutases family. O86374 No intermediary metabolism and respiration O86374 O86374 GO:0004615,GO:0005975 5.4.2.8 O86374 NC_000962.3 Mycobrowser_v5 CDS 3641535 3642881 . + 0 Rv3262 fbiB Required for coenzyme F420 production: involved in the conversion of FO into F420. Probable F420 biosynthesis protein FbiB Rv3262, (MTCY71.02), len: 448 aa. Probable fbiB, F420 biosynthesis protein, equivalent to FBIB F420 biosynthesis protein fbiB from Mycobacterium bovis BCG (see citations below). Also equivalent to Q9CCK2|ML0758 putative oxidoreductase from Mycobacterium leprae (457 aa), FASTA scores: opt: 2411, E(): 3.5e-137, (82.25% identity in 445 aa overlap). Also similar to Q9KZK8|SCE34.18 putative oxidoreductase from Streptomyces coelicolor (443 aa), FASTA scores: opt: 1180, E(): 2.2e-63, (51.75% identity in 433 aa overlap); other oxidoreductases in C-terminus; and several hypothetical bacterial proteins. P9WP79 No intermediary metabolism and respiration P96867 P9WP79 GO:0043773,GO:0009108,GO:0046872,GO:0016491,GO:0005525 6.3.2.- P9WP79 NC_000962.3 Mycobrowser_v5 CDS 3910947 3911675 . - 0 Rv3493c Rv3493c Unknown Conserved hypothetical Mce associated alanine and valine rich protein Rv3493c, (MTCY13E12.46c), len: 242 aa. Conserved hypothetical Mce-associated ala-, val-rich protein, showing weak similarity to O07422|Z97050|Rv0178|MTCI28.18 hypothetical 25.9 KDA protein (near Mce operon1) from Mycobacterium tuberculosis (244 aa), FASTA scores: opt: 163, E(): 0.046, (24.65% identity in 211 aa overlap). I6X7G4 No conserved hypotheticals I6X7G4 I6X7G4 NC_000962.3 Mycobrowser_v5 CDS 3645979 3646884 . - 0 Rv3265c wbbL1 Probably involved in cell wall arabinogalactan linker formation: Uses dTDP-L-rhamnose as substrate to insert the rhamnosyl residue into the cell wall. Seems to be essential for mycobacterial viability. dTDP-RHA:a-D-GlcNAc-diphosphoryl polyprenol, a-3-L-rhamnosyl transferase WbbL1 (alpha-L-rhamnose-(1->3)-alpha-D-GlcNAc(1->P)-P-decaprenyl) Rv3265c, (MTCY71.05c), len: 301 aa. wbbL1, dTDP-RHA:a-D-GlcNAc-diphosphoryl polyprenol a-3-L-rhamnosyl transferase (see citations below), equivalent to Q9CCK7|WBBL|ML0752 putative dTDP-rhamnosyl transferase from Mycobacterium leprae (308 aa), FASTA scores: opt: 1788, E(): 3e-104, (85.05% identity in 301 aa overlap); and Q9RN50|WBBL|Q9RN49 (see note * below) dTDP-RHA:a-D-GlcNAc-diphosphoryl polyprenol, a-3-L-rhamnosyl transferase from Mycobacterium smegmatis (296 aa), FASTA scores: opt: 1494, E(): 6.1e-86, (72.35% identity in 293 aa overlap). Note that previously known as wbbL. [* Note: unpublished (experimental study on Mycobacterium smegmatis). Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases - The cell wall arabinogalactan linker formation enzyme, dTDP-Rha:a-D-GlcNAc-diphosphoryl polyprenol, a-3-L-rhamnosyl transferase is essential for mycobacterial viability - Mills J.A., Motichka K., Jucker M., Wu H.P., Uhlic B.C., Stern R.J., Scherman M.S., Vissa V.D., Yan W., Pan F., Kimbrel S., Kundu M., McNeil M.]. P9WMY3 No cell wall and cell processes Q7D5T2 P9WMY3 GO:0016740 2.4.-.- NC_000962.3 Mycobrowser_v5 CDS 3646895 3647809 . - 0 Rv3266c rmlD Involved in dTDP-L-rhamnose biosynthesis: converts dTDP-6-deoxy-L-lyxo-4-hexulose to dTDP-L-rhamnose with the concomitant oxidation of NADPH to NADP+ [catalytic activity: dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH = dTDP-L-rhamnose + NADP+]. dTDP-6-deoxy-L-lyxo-4-hexulose reductase RmlD (dTDP-rhamnose modification protein) (dTDP-rhamnose biosynthesis protein) (dTDP-rhamnose synthase) Rv3266c, (MTCY71.06c), len: 304 aa. RmlD, dTDP-6-deoxy-L-lyxo-4-hexulose reductase (dTDP-rhamnose modification protein) (see citations below), highly similar to Q9CCK8 putative dTDP-rhamnose modification protein from Mycobacterium leprae (311 aa), FASTA scores, opt: 1440, E(): 1.1e-78, (74.7% identity in 312 aa overlap); and similar to several dTDP-4-dehydrorhamnose reductase e.g. STRL_STRGR|P29781 from Streptomyces griseus (304 aa), FASTA scores, opt: 788, E(): 0, (47.4% identity in 304 aa overlap). P9WH09 No intermediary metabolism and respiration P96871 P9WH09 GO:0008831,GO:0045226,GO:0055114 1.1.1.133 EMB P9WH09 NC_000962.3 Mycobrowser_v5 CDS 3647885 3649381 . + 0 Rv3267 Rv3267 Function unknown Conserved protein (CPSA-related protein) Rv3267, (MTCY71.07), len: 498 aa. Conserved protein, CPSA-related protein, equivalent to Q9CCK9|ML0750 hypothetical protein from Mycobacterium leprae (489 aa), FASTA scores: opt: 2523, E(): 5e-138, (78.9% identity in 498 aa overlap); and Q50160|CPSA (hypothetical protein CPSA) from Mycobacterium leprae (516 aa), FASTA scores: opt: 868, E(): 1.2e-42, (34.7% identity in 507 aa overlap). Also similar to O06347|CPSA|Rv3484|MTCY13E12.37 CPSA from Mycobacterium tuberculosis (512 aa), FASTA scores: opt: 928, E(): 4.2e-46, (37.35% identity in 498 aa overlap); and O53834|Rv0822c|MTV043.14c hypothetical 72.9 KDA protein from Mycobacterium tuberculosis (684 aa), FASTA scores: opt: 434, E(): 1.5e-17, (30.9% identity in 541 aa overlap). Also similar to Q9KZK0|SCE34.26 conserved hypothetical protein from Streptomyces coelicolor (507 aa), FASTA scores: opt: 437, E(): 8.1e-18, (28.55% identity in 469 aa overlap); O68907 FRNA protein from Streptomyces roseofulvus (770 aa), FASTA scores: opt: 388, E(): 7.6e-15, (32.6% identity in 267 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A. Predicted to be an outer membrane protein (See Song et al., 2008). P96872 No conserved hypotheticals P96872 P96872 P96872 NC_000962.3 Mycobrowser_v5 CDS 3900493 3901182 . - 0 Rv3481c Rv3481c Unknown Probable integral membrane protein Rv3481c, (MTCY13E12.34c), len: 229 aa. Probable integral membrane protein. No real similarity with others. I6XHC3 No cell wall and cell processes O06344 I6XHC3 NC_000962.3 Mycobrowser_v5 CDS 3650526 3652682 . + 0 Rv3270 ctpC Metal cation-transporting ATPase; possibly catalyzes the transport of undetermined metal cation with the hydrolyse of ATP [catalytic activity: ATP + H(2)O + undetermined metal cation(in) = ADP + phosphate + undetermined metal cation(out)]. Probable metal cation-transporting P-type ATPase C CtpC Rv3270, (MT3370, MTCY71.10), len: 718 aa. Probable ctpC, metal cation-transport ATPase P-type, integral membrane protein, equivalent to Q9CCL1|CTPC|ML0747 putative cation transport ATPase from Mycobacterium leprae (725 aa), FASTA scores: opt: 3908, E(): 0, (85.95% identity in 713 aa overlap). Also similar to O66027|MTAA metal transporting ATPase MTA72 from Mycobacterium tuberculosis (680 aa), FASTA scores: opt: 3756, E(): 5.5e-213, (91.45% identity in 679 aa overlap); and to other ATPases e.g. Q9ZHC7|SILP_SALTY putative cation transporting P-type ATPase from Salmonella typhimurium (824 aa), FASTA scores: opt: 1145, E(): 1.3e-59, (36.55% identity in 643 aa overlap); Q9HX93|PA3920 probable metal transporting P-type ATPase from Pseudomonas aeruginosa (792 aa), FASTA scores: opt: 1140, E(): 2.4e-59, (35.95% identity in 745 aa overlap); etc. Contains PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases), subfamily IB. P9WPT5 No cell wall and cell processes P0A502 P9WPT5 GO:0006754,GO:0015662,GO:0016021,GO:0046872,GO:0030001,GO:0005886,GO:0005524 3.6.3.- P9WPT5 NC_000962.3 Mycobrowser_v5 CDS 3774136 3774504 . - 0 Rv3363c Rv3363c Function unknown Conserved hypothetical protein Rv3363c, (MTV004.20c), len: 122 aa. Conserved hypothetical protein, similar to others from Streptomyces coelicolor e.g. O86523|SC1C2.23c (132 aa), FASTA scores: opt: 236, E(): 9e-09, (38.5% identity in 122 aa overlap); O86520|SC1C2.19c (190 aa), FASTA scores: opt: 231, E(): 2.7e-08, (41.0% identity in 122 aa overlap); Q9X834|SC9B1.14c (119 aa), FASTA scores: opt: 188, E(): 1.1e-05, (37.5% identity in 120 aa overlap); Q9ADJ4|SCBAC14E8.05 (113 aa), FASTA scores: opt: 167, E(): 0.00025, (33.05% identity in 109 aa overlap); etc. O50392 No conserved hypotheticals O50392 O50392 NC_000962.3 Mycobrowser_v5 CDS 3796035 3796361 . - 0 Rv3381c Rv3381c Involved in the transposition of the insertion sequence IS6110. Probable transposase for insertion sequence element IS6110 (fragment) Rv3381c, (MTV004.39c), len: 108 aa. Putative Transposase for IS6110 (fragment). Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv3380c and Rv3381c, the sequence UUUUAAAG (directly upstream of Rv3380c) maybe responsible for such a frameshifting event (see McAdam et al., 1990). P9WKH5 No insertion seqs and phages P0C5G9 P9WKH5 GO:0004803,GO:0006313,GO:0003677 P9WKH5 NC_000962.3 Mycobrowser_v5 CDS 3656920 3658089 . - 0 Rv3274c fadE25 Function unknown, but involved in lipid degradation [catalytic activity: acyl-CoA + ETF = 2,3-dehydroacyl-CoA + reduced ETF]. Probable acyl-CoA dehydrogenase FadE25 Rv3274c, (MTCY71.14c), len: 389 aa. Probable fadE25, Acyl-CoA Dehydrogenase, equivalent to P46703|ACDP_MYCLE|FADE25|ACD|ML0737|B1308_F1_34 probable acyl-CoA dehydrogenase FADE25 from Mycobacterium leprae (389 aa), FASTA scores: opt: 2394, E(): 3.8e-143, (92.05% identity in 389 aa overlap). Also similar to many e.g. Q9RIQ5|fade fatty acid acyl-CoA dehydrogenase from Streptomyces lividans (385 aa), FASTA scores: opt: 1692, E(): 4.9e-99, (67.35% identity in 383 aa overlap); P45867|ACDA_BACSU|ACD from Bacillus subtilis (379 aa), FASTA scores: opt: 1212, E(): 7.2e-69, (51.85% identity in 376 aa overlap); Q9K6D1|ACDA|BH3798 from Bacillus halodurans (380 aa), FASTA scores: opt: 1209, E(): 1.1e-68, (51.7% identity in 377 aa overlap); P52042|ACDS_CLOAB|BCD from Clostridium acetobutylicum (379 aa), FASTA scores: opt: 1056, E(): 4.6e-59, (44.6% identity in 379 aa overlap); etc. Contains PS00072 Acyl-CoA dehydrogenases signature 1, PS00073 Acyl-CoA dehydrogenases signature 2. Belongs to the acyl-CoA dehydrogenases family. P9WQG1 No lipid metabolism P63427 P9WQG1 GO:0055114,GO:0003995 1.3.99.- P9WQG1 NC_000962.3 Mycobrowser_v5 CDS 3663689 3664222 . + 0 Rv3281 accE5 Involved in long-chain fatty acid synthesis Probable bifunctional protein acetyl-/propionyl-coenzyme A carboxylase (epsilon chain) AccE5 Rv3281, (MTCY71.21), len: 177 aa. Probable accE5, bifunctional acetyl-/propionyl-coenzyme A carboxylase, epsilon chain, equivalent (but longer 14 aa and with a gap between aa 82-102) to AAK47723|MT3380 from Mycobacterium tuberculosis strain CDC1551 (142 aa), FASTA scores: opt: 830, E(): 3.1e-40, (86.5% identity in 163 aa overlap). C-terminus highly similar to Q49671|B1308_C3_211|ML0730 from Mycobacterium leprae (84 aa), FASTA scores: opt: 393, E(): 7.6e-16, (68.95% identity in 87 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). AccA3 (Rv3285), AccD5 (Rv3280), AccD4 (Rv3799), and AccE5 (Rv3281) form a biotin-dependent acyl-CoA carboxylase in M. tuberculosis H37Rv (See Oh et al., 2006). P96886 No lipid metabolism P96886 NC_000962.3 Mycobrowser_v5 CDS 3672328 3673575 . + 0 Rv3292 Rv3292 Function unknown Conserved hypothetical protein Rv3292, (MTCY71.32), len: 415 aa. Conserved hypothetical protein, similar to P76097|YDCJ_ECOLI|B1423 hypothetical 51.0 KDA protein from Escherichia coli strain K12 (447 aa), FASTA scores: opt: 747, E(): 5.6e-39, (38.55% identity in 449 aa overlap); BAB35451|ECS2028 hypothetical 51.0 KDA protein from Escherichia coli strain O157:H7 (447 aa), FASTA scores: opt: 744, E(): 8.6e-39, (38.3% identity in 449 aa overlap); AAG56352|Z2297 protein from Escherichia coli O157:H7 EDL933 (212 aa), FASTA scores: opt: 454, E(): 4.6e-21, (41.75% identity in 206 aa overlap); and similar in part with Q49664|B1308_C1_136 from Mycobacterium leprae (71 aa), FASTA scores: opt: 305, E(): 3.2e-12, (70.0% identity in 70 aa overlap). P9WL01 No conserved hypotheticals P65065 P9WL01 P9WL01 NC_000962.3 Mycobrowser_v5 CDS 3659878 3660696 . + 0 Rv3277 Rv3277 Unknown Probable conserved transmembrane protein Rv3277, (MTCY71.17), len: 272 aa. Probable conserved transmembrane protein, equivalent, but longer 49 aa, to Q49673|B1308_C1_121|ML0734 putative membrane protein from Mycobacterium leprae (228 aa), FASTA scores: opt: 1266, E(): 6.1e-78, (84.2% identity in 228 aa overlap). Also similar to various proteins (principally unknowns) e.g. Q9KZ84|SCE25.02 putative integral membrane protein from Streptomyces coelicolor (190 aa), FASTA scores: opt: 197, E(): 3.6e-06, (32.0% identity in 150 aa overlap); BAB50058|MLL3086 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (136 aa), FASTA scores: opt: 176, E(): 6.9e-05, (34.7% identity in 147 aa overlap); O29640|AF0615 hypothetical protein from Archaeoglobus fulgidus (129 aa), FASTA scores: opt: 120, E(): 0.38, (23.35% identity in 120 aa overlap); Q9KJU8|GTCA teichoic acid glycosylation protein from Listeria innocua (145 aa), FASTA scores: opt: 117, E(): 0.67, (23.85% identity in 151 aa overlap); etc. Equivalent to AAK47718 from Mycobacterium tuberculosis strain CDC1551 (256 aa) but longer 16 aa. Contains PS00044 Bacterial regulatory proteins, lysR family signature. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P96882 No cell wall and cell processes P96882 P96882 GO:0000271,GO:0006810,GO:0016021 P96882 NC_000962.3 Mycobrowser_v5 CDS 3660651 3661169 . - 0 Rv3278c Rv3278c Unknown Probable conserved transmembrane protein Rv3278c, (MTCY71.18c), len: 172 aa. Probable conserved transmembrane protein, equivalent to Q9CCL2|ML0733 putative membrane protein from Mycobacterium leprae (172 aa), FASTA scores: opt: 1024, E(): 6e-61, (83.15% identity in 172 aa overlap); and Q49672|B1308_F2_67 hypothetical protein from Mycobacterium leprae (181 aa), FASTA scores: opt: 1024, E(): 6.3e-61, (83.15% identity in 172 aa overlap) (this is certainly the same putative protein but with N-terminus longer). Also some similarity to other hypothetical proteins (generally membrane proteins) e.g. O26822|MTH726 hypothetical protein from Methanobacterium thermoautotrophicum (204 aa), FASTA scores: opt: 147, E(): 0.0079, (24.6% identity in 187 aa overlap); Q9X8H4|SCE9.01 hypothetical 47.7 KDA protein (fragment) from Streptomyces coelicolor (436 aa), FASTA scores: opt: 151, E(): 0.0079, (28.1% identity in 153 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P96883 No cell wall and cell processes P96883 P96883 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 3711749 3713461 . + 0 Rv3327 Rv3327 Involved in the transposition in the insertion sequence element IS1547. Probable transposase fusion protein Rv3327, (MTV016.27), len: 570 aa. Probable fusion protein. Indeed, N-terminal part corresponds to entire O07269 transposase of IS1547 (383 aa), and C-terminal part identical to MTCI249B.03c (210 aa). N-terminal part is identical to MTV042_7 (188 aa); C-terminal part (aa 378-570) is similar to hypothetical 20.5 kDa protein from Escherichia coli P76222|YNJA_ECOLI (182 aa), FASTA scores: opt: 292, E(): 5.3e-11, (32.6% identity in 181 aa overlap). O53377 No insertion seqs and phages O53377 O53377 GO:0004803,GO:0006313,GO:0003677 O53377 NC_000962.3 Mycobrowser_v5 CDS 3665818 3666249 . + 0 Rv3284 Rv3284 Function unknown Conserved hypothetical protein Rv3284, (MTCY71.24, unknown), len: 143 aa. Conserved hypothetical protein, with similarity to other bacterial hypothetical proteins e.g. Q9RXU0|DR0216 from Deinococcus radiodurans (147 aa), FASTA scores: opt: 425, E(): 9.1e-21, (46.55% identity in 146 aa overlap); BAB37094|ECS3671 from Escherichia coli strain O157:H7 (147 aa), FASTA scores: opt: 187, E(): 2.2e-05, (29.5% identity in 139 aa overlap); AAG57925|YGDK from Escherichia coli strain O157:H7 EDL933 (147 aa), FASTA scores: opt: 187, E(): 2.2e-05, (32.05% identity in 139 aa overlap); etc. P9WGC3 No conserved hypotheticals P67123 P9WGC3 P9WGC3 NC_000962.3 Mycobrowser_v5 CDS 3662062 3663708 . + 0 Rv3280 accD5 Key enzyme in the catabolic pathway of odd-chain fatty acids, isoleucine, threonine, methionine, and valine [catalytic activity: ATP + propionyl-CoA + CO(2) + H(2)O = ADP + orthophosphate + methylmalonyl-CoA.] Probable propionyl-CoA carboxylase beta chain 5 AccD5 (pccase) (propanoyl-CoA:carbon dioxide ligase) Rv3280, (MTCY71.20, pccB), len: 548 aa. Probable accD5, propionyl-CoA carboxylase beta chain 5, equivalent to P53002|PCCB_MYCLE|ACCD5|ML0731|B1308_C1_125 probable propionyl-CoA carboxylase beta chain 5 from Mycobacterium leprae (549 aa), FASTA scores: opt: 3241, E(): 4e-192, (88.7% identity in 549 aa overlap). Also similar to many e.g. O87201|DTSR2 DTSR2 protein involved in glutamate production from Corynebacterium glutamicum (Brevibacterium flavum) (537 aa), FASTA scores: opt: 2604, E(): 6.9e-153, (74.1% identity in 529 aa overlap) (see Kimura et al., 1996); P53003|PCCB_SACER from Saccharopolyspora erythraea (Streptomyces erythraeus) (546 aa), FASTA scores: opt: 2466, E(): 2.2e-144, (70.2% identity in 530 aa overlap); O88155|DTSR1 DTSR1 protein from Corynebacterium glutamicum (Brevibacterium flavum) (543 aa), FASTA scores: opt: 2375, E(): 8.8e-139, (67.1% identity in 529 aa overlap; Q9X4K7|PCCB from Streptomyces coelicolor (530 aa), FASTA scores: opt: 2360, E(): 7.3e-138, (67.9% identity in 533 aa overlap); O24789|mxpccb from Myxococcus xanthus (524 aa), FASTA scores: opt: 1868, E(): 1.5e-107, (56.85% identity in 524 aa overlap); etc. Also similar with methylmalonyl-CoA decarboxylases e.g. O59018|PH1287 from Pyrococcus horikoshii (522 aa), FASTA scores: opt: 1841, E(): 6.7e-106, (54.15% identity in 528 aa overlap). Also similarity with MTCY427.28 (43.8% identity in 434 aa overlap). Belongs to the ACCD/PCCB family. AccA3 (Rv3285), AccD5 (Rv3280), AccD4 (Rv3799), and AccE5 (Rv3281) form a biotin-dependent acyl-CoA carboxylase in M. tuberculosis H37Rv (See Oh et al., 2006). P9WQH7 No lipid metabolism 2A7S,2BZR P96885 P9WQH7 GO:0004658,GO:0005524 6.4.1.3 P9WQH7 NC_000962.3 Mycobrowser_v5 CDS 3664928 3665821 . + 0 Rv3283 sseA Possibly a sulfotransferase involved in the formation of thiosulfate [catalytic activity: thiosulfate + cyanide = sulfite + thiocyanate]. Probable thiosulfate sulfurtransferase SseA (rhodanese) (thiosulfate cyanide transsulfurase) (thiosulfate thiotransferase) Rv3283, (MTCY71.23), len: 297 aa. Probable sseA, thiosulfate sulfurtransferase, equivalent P46700|THT2_MYCLE|SSEA|ML0728|B1308_C1_127 putative thiosulfate sulfurtransferase SSEA from Mycobacterium leprae (296 aa), FASTA scores: opt: 1742, E(): 5.5e-108, (83.45% identity in 296 aa overlap). Also highly similar to others e.g. Q9RXT9|DR0217 from Deinococcus radiodurans (286 aa), FASTA scores: opt: 1057, E(): 1.2e-62, (53.86% identity in 273 aa overlap); P16385|THTR_SACER|CYSA from Saccharopolyspora erythraea (Streptomyces erythraeus) (281 aa), FASTA scores: opt: 1006, E(): 2.7e-59, (51.25% identity in 277 aa overlap); P71121|THTR_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (225 aa), FASTA scores: opt: 897, E(): 3.6e-52, (59.05% identity in 215 aa overlap); etc. Also highly similar to O05793|CYSA1|CYSA|Rv3117|MT3199|MTCY164.27|CYSA2|RV0815c|MT0837|MTV043. 07c|THTR_MYCTU putative thiosulfate sulfurtransferase from Mycobacterium tuberculosis (277 aa), FASTA scores: opt: 955, E(): 6.3e-56, (50.2% identity in 271 aa overlap); and Q50036|THTR_MYCLE|CYSA|CYSA3|ML2198 putative thiosulfate sulfurtransferase from Mycobacterium leprae (277 aa), FASTA scores: opt: 931, E(): 2.5e-54, (48.9% identity in 276 aa overlap). Shows some similarity to MTCY339.19c (30.3% identity in 254 aa overlap). Contains PS00683 Rhodanese C-terminal signature. Belongs to the rhodanese family. Thought to be differentially expressed within host cells (see Triccas et al., 1999). P9WHF7 No intermediary metabolism and respiration 3HZU P96888 P9WHF7 GO:0004792,GO:0008272 2.8.1.1 P9WHF7 NC_000962.3 Mycobrowser_v5 CDS 3666357 3668159 . + 0 Rv3285 accA3 Involved in long-chain fatty acid synthesis (at the first step). Carries two functions: biotin carboxyl carrier protein and biotin carboxyltransferase [catalytic activity: ATP + biotin-carboxyl-carrier protein + CO(2) = ADP + orthophosphate + carboxybiotin-carboxyl-carrier protein]. Probable bifunctional protein acetyl-/propionyl-coenzyme A carboxylase (alpha chain) AccA3: biotin carboxylase + biotin carboxyl carrier protein (BCCP) Rv3285, (MTCY71.25), len: 600 aa. Probable accA3, bifunctional protein acetyl-/propionyl-coenzyme A carboxylase, alpha chain (see citations below) equivalent to P46392|BCCA_MYCLE|BCCA|ML0726|B1308_C1_129 acetyl-/propionyl-coenzyme A carboxylase alpha chain from Mycobacterium leprae (598 aa), FASTA scores: opt: 3510, E(): 1.1e-196, (89.3% identity in 601 aa overlap). Also highly similar to other proteins e.g. P71122|ACCBC acyl coenzyme A carboxylase from Corynebacterium glutamicum (Brevibacterium flavum) (591 aa), FASTA scores: opt: 2776, E(): 5.6e-154, (71.95% identity in 592 aa overlap); Q54119|BCPA2 biotin carboxylase and biotin carboxyl carrier protein from Saccharopolyspora erythraea (Streptomyces erythraeus) (591 aa), FASTA scores: opt: 2723, E(): 6.7e-151, (70.5% identity in 590 aa overlap); Q54105|BCPA biotin carboxylase and biotin carboxyl carrier protein from Saccharopolyspora erythraea (Streptomyces erythraeus) (597 aa), FASTA scores: opt: 2721, E(): 8.9e-151, (70.05% identity in 594 aa overlap); Q9EWV4|2SCK31.20 putative acyl-CoA carboxylase complex a subunit from Streptomyces coelicolor (590 aa), FASTA scores: opt: 2626, E(): 2.9e-145, (68.25% identity in 595 aa overlap); etc. Contains PS00867 Carbamoyl-phosphate synthase subdomain signature 2, PS00188 Biotin-requiring enzymes attachment site. Similar to other biotin-dependent enzymes and carbamoyl-phosphate synthetases. AccA3 (Rv3285), AccD5 (Rv3280), AccD4 (Rv3799), and AccE5 (Rv3281) form a biotin-dependent acyl-CoA carboxylase in M. tuberculosis H37Rv (See Oh et al., 2006). P96890 No lipid metabolism P96890 P96890 GO:0009374,GO:0004075,GO:0008152,GO:0005524 6.3.4.14 P96890 NC_000962.3 Mycobrowser_v5 CDS 3668951 3669388 . - 0 Rv3287c rsbW Binds to sigma and blocks its ability to form an RNA polymerase holoenzyme. Regulates negatively SIGF|Rv3286c, and negatively regulated by Rv1365c|RSFA and Rv3687C|RSFB. Anti-sigma factor RsbW (sigma negative effector) Rv3287c, (MTCY71.27c), len: 145 aa. RsbW (alternate gene name: usfX), anti-sigma factor (see citations below), similar to Q49667|B1308_F3_89 from Mycobacterium leprae (75 aa), FASTA scores: opt: 308, E(): 2.5e-15, (72.2% identity in 72 aa overlap); Q9R3X8|PRS1|USHX|PRS PRS1 protein (anti-sigma factor) from Streptomyces coelicolor (137 aa), FASTA scores: opt: 184, E(): 3.7e-06, (36.8% identity in 106 aa overlap); O50231 putative sigma-B regulator from Bacillus licheniformis (160 aa), FASTA scores: opt: 122, E(): 0.13, (23.9% identity in 92 aa overlap); and P17904|RSBW_BACSU anti-sigma B factor (sigma-B negative effector RSBW) from Bacillus subtilis (160 aa), FASTA scores: opt: 108, E(): 1.3, (21.25% identity in 127 aa overlap). Equivalent to AAK47729 from Mycobacterium tuberculosis strain CDC1551 (145 aa) but longer 99 aa. Induction by heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation. N-terminus shortened since first submission (previously 242 aa). Binds ATP, GTP. P9WGX7 No information pathways P9WGX7 P9WGX7 NC_000962.3 Mycobrowser_v5 -10_signal 3669523 3669528 . - 0 Rv3287c rsbW Binds to sigma and blocks its ability to form an RNA polymerase holoenzyme. Regulates negatively SIGF|Rv3286c, and negatively regulated by Rv1365c|RSFA and Rv3687C|RSFB. Anti-sigma factor RsbW (sigma negative effector) Rv3287c, (MTCY71.27c), len: 145 aa. RsbW (alternate gene name: usfX), anti-sigma factor (see citations below), similar to Q49667|B1308_F3_89 from Mycobacterium leprae (75 aa), FASTA scores: opt: 308, E(): 2.5e-15, (72.2% identity in 72 aa overlap); Q9R3X8|PRS1|USHX|PRS PRS1 protein (anti-sigma factor) from Streptomyces coelicolor (137 aa), FASTA scores: opt: 184, E(): 3.7e-06, (36.8% identity in 106 aa overlap); O50231 putative sigma-B regulator from Bacillus licheniformis (160 aa), FASTA scores: opt: 122, E(): 0.13, (23.9% identity in 92 aa overlap); and P17904|RSBW_BACSU anti-sigma B factor (sigma-B negative effector RSBW) from Bacillus subtilis (160 aa), FASTA scores: opt: 108, E(): 1.3, (21.25% identity in 127 aa overlap). Equivalent to AAK47729 from Mycobacterium tuberculosis strain CDC1551 (145 aa) but longer 99 aa. Induction by heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation. N-terminus shortened since first submission (previously 242 aa). Binds ATP, GTP. P9WGX7 No information pathways P9WGX7 P9WGX7 NC_000962.3 Mycobrowser_v5 -35_signal 3669546 3669549 . - 0 Rv3287c rsbW Binds to sigma and blocks its ability to form an RNA polymerase holoenzyme. Regulates negatively SIGF|Rv3286c, and negatively regulated by Rv1365c|RSFA and Rv3687C|RSFB. Anti-sigma factor RsbW (sigma negative effector) Rv3287c, (MTCY71.27c), len: 145 aa. RsbW (alternate gene name: usfX), anti-sigma factor (see citations below), similar to Q49667|B1308_F3_89 from Mycobacterium leprae (75 aa), FASTA scores: opt: 308, E(): 2.5e-15, (72.2% identity in 72 aa overlap); Q9R3X8|PRS1|USHX|PRS PRS1 protein (anti-sigma factor) from Streptomyces coelicolor (137 aa), FASTA scores: opt: 184, E(): 3.7e-06, (36.8% identity in 106 aa overlap); O50231 putative sigma-B regulator from Bacillus licheniformis (160 aa), FASTA scores: opt: 122, E(): 0.13, (23.9% identity in 92 aa overlap); and P17904|RSBW_BACSU anti-sigma B factor (sigma-B negative effector RSBW) from Bacillus subtilis (160 aa), FASTA scores: opt: 108, E(): 1.3, (21.25% identity in 127 aa overlap). Equivalent to AAK47729 from Mycobacterium tuberculosis strain CDC1551 (145 aa) but longer 99 aa. Induction by heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation. N-terminus shortened since first submission (previously 242 aa). Binds ATP, GTP. P9WGX7 No information pathways P9WGX7 P9WGX7 NC_000962.3 Mycobrowser_v5 CDS 3669586 3669999 . - 0 Rv3288c usfY Function unknown Putative protein UsfY Rv3288c, (MTCY71.28c), len: 137 aa. UsfY, putative protein (see citation below). Has no significant homologues. May not be contranscribed with the usfX and sigF proteins. L7N685 No conserved hypotheticals L7N685 NC_000962.3 Mycobrowser_v5 CDS 3670034 3670411 . - 0 Rv3289c Rv3289c Unknown Possible transmembrane protein Rv3289c, (MTCY71.29c), len: 125 aa. Possible transmembrane protein, showing slight similarity to other membrane proteins or glycoproteins. P96894 No cell wall and cell processes P96894 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 3671845 3672297 . - 0 Rv3291c lrpA Involved in transcriptional mechanism. Probable transcriptional regulatory protein LrpA (Lrp/AsnC-family) Rv3291c, (MTCY71.31c), len: 150 aa. Probable lrpA, transcriptional regulator Lrp/AsnC-family, similar to other regulatory proteins e.g. Q9RKY4|SC6D7.14 from Streptomyces coelicolor (165 aa), FASTA scores: opt: 503, E(): 9.1e-26, (50.35% identity in 143 aa overlap); Q9KYP0|SCD69.13 from Streptomyces coelicolor (167 aa), FASTA scores: opt: 310, E(): 2.7e-13, (37.2% identity in 129 aa overlap); BAB50701|MLL3910 from Rhizobium loti (Mesorhizobium loti) (152 aa), FASTA scores: opt: 282, E(): 1.6e-11, (39.55% identity in 129 aa overlap); O87635|LRP_KLEAE from Klebsiella aerogenes (163 aa), FASTA scores: opt: 279, E(): 2.5e-11, (38.1% identity in 147 aa overlap); etc. Contains helix-turn-helix motif at aa 22-43 (+3.94 SD). Could belong to the Lrp/AsnC family of transcriptional regulators. I6YBQ3 No regulatory proteins 2IVM,2QZ8,2VBW,2VBX,2VBY,2VBZ,2VC0,2VC1,2W24,2W25,2W29 P96896 I6YBQ3 GO:0006355,GO:0043565,GO:0006350,GO:0003700,GO:0005622 I6YBQ3 NC_000962.3 Mycobrowser_v5 CDS 3894093 3894389 . + 0 Rv3477 PE31 Function unknown PE family protein PE31 Rv3477, (MTCY13E12.30), len: 98 aa. PE31, Member of the Mycobacterium tuberculosis PE family (see Brennan & Delogu 2002), similar to O53941|Rv1791|MTV049.13 (99 aa), FASTA scores: opt: 373, E(): 4.3e-18, (64.65% identity in 99 aa overlap); MTCI364.07; MTCY21C12.10c; MTCY1A11.25c; MTC1A11.04; MTCY359.33; etc. I6YG87 No PE/PPE Q7D5G7 I6YG87 I6YG87 NC_000962.3 Mycobrowser_v5 CDS 3800786 3801463 . + 0 Rv3387 Rv3387 Involved in the transposition of the insertion sequence IS1560. Possible transposase Rv3387, (MTV004.45), len: 225 aa. Possible transposase, showing very weak similarity to other is element proteins, and similar to various hypothetical proteins. O50414 No insertion seqs and phages O50414 O50414 NC_000962.3 Mycobrowser_v5 CDS 3675186 3675995 . - 0 Rv3294c Rv3294c Function unknown Conserved hypothetical protein Rv3294c, len: 269 aa. Conserved hypothetical protein, similar to several conserved hypothetical proteins from Mycobacterium tuberculosis: O07781|Rv0597c (411 aa), FASTA scores: opt: 682, E(): 3.6e-37, (44.85% identity in 243 aa overlap); O53329|Rv3179 (454 aa), FASTA scores: opt: 561, E(): 3.3e-29, (42.20% identity in 218 aa overlap); Q10849|YK08_MYCTU|Rv2008c (441 aa), FASTA scores: opt: 194, E(): 3.9e-05, (30.10% identity in 239 aa overlap). Also some similarity with proteins from other organisms. Replace previous Rv3294 on opposite strand. L7N658 No conserved hypotheticals L7N658 L7N658 NC_000962.3 Mycobrowser_v5 CDS 3676775 3681316 . + 0 Rv3296 lhr Has both ATPase and helicase activities. Probable ATP-dependent helicase Lhr (large helicase-related protein) Rv3296, (MTCY71.36), len: 1513 aa. Probable lhr, ATP-dependent helicase, similar to others e.g. P30015|LHR_ECOLI|RHLF|B1653 from Escherichia coli stain K12 (1538 aa), FASTA scores: opt: 2930, E(): 1.5e-159, (47.55% identity in 1569 aa overlap); AAG56642|LHR from Escherichia coli stain O157:H7 EDL933 (1538 aa), FASTA scores: opt: 2930, E(): 1.5e-159, (47.6% identity in 1561 aa overlap); O86821|SC7C7.16c from Streptomyces coelicolor (1690 aa), FASTA scores: opt: 2919, E(): 7e-159, (53.55% identity in 1703 aa overlap); Q9HYW9|PA3272 from Pseudomonas aeruginosa (1448 aa), FASTA scores: opt: 907, E(): 6.2e-44, (35.85% identity in 1512 aa overlap); etc. Similar to dead/DEAH box helicase family and to helicase C-terminal domain. Contains PS00017 ATP/GTP-binding site motif A and possible helix-turn-helix motif. P96901 No information pathways P96901 P96901 GO:0008026,GO:0003676,GO:0005524 3.6.1.- P96901 NC_000962.3 Mycobrowser_v5 CDS 3681320 3682087 . + 0 Rv3297 nei Involved in damage reversal. DNA N-glycosylase with an AP lyase activity. Required for the repair of oxidative DNA damage (oxidized pyrimidines). Probable endonuclease VIII Nei Rv3297, (MTCY71.37, MT3396), len: 255 aa. Probable nei, endonuclease VIII (see citation below), similar to others e.g. O86820|END8_STRCO|NEI|SC7C7.15c from Streptomyces coelicolor (276 aa), FASTA scores: opt: 770, E(): 1.2e-42, (50.35% identity in 268 aa overlap); P50465|END8_ECOLI|NEI|B0714 from Escherichia coli strain K12 (262 aa), FASTA scores: opt: 310, E(): 6.3e-13, (28.1% identity in 267 aa overlap); AAG55037|NEI from Escherichia coli strain O157:H7 EDL933 (263 aa), FASTA scores: opt: 301, E(): 2.4e-12, (27.7% identity in 267 aa overlap); etc. Belongs to the FPG family. P9WNC1 No information pathways P64156 P9WNC1 GO:0006284,GO:0003684,GO:0016799,GO:0006289,GO:0008270,GO:0003906 4.2.99.18,3.2.2.- P9WNC1 NC_000962.3 Mycobrowser_v5 CDS 3683051 3685963 . - 0 Rv3299c atsB Generates sulfate and phenol from phenol sulfate [catalytic activity: a phenol sulfate + H(2)O = a phenol + sulfate]. Probable arylsulfatase AtsB (aryl-sulfate sulphohydrolase) (sulfatase) Rv3299c, (MTCI418A.01c, MTCY71.39c), len: 970 aa. Probable atsB, arylsulfatase, similar to P51691|ARS_PSEAE|ATSA|PA0183 (alias CAA88421|ATSA) from Pseudomonas aeruginosa (535 aa), FASTA scores: opt: 645, E(): 5.8e-31, (32.0% identity in 550 aa overlap); Q9L4Y2|ATSA from Klebsiella pneumoniae (577 aa), FASTA scores: opt: 504, E(): 1.7e-22, (26.3% identity in 566 aa overlap); and P20713|ATSA|ARS_KLEAE (precursor) from Klebsiella pneumoniae (464 aa), FASTA scores: opt: 502, E(): 1.8e-22, (26.85% identity in 451 aa overlap). Also similar to Mycobacterium tuberculosis proteins O06776|MTI376.13c|ATSD|Rv0663 (787 aa) (43.6% identity in 796 aa overlap) and P95059|MTCY210.30|ATSA|R0711 (787 aa) (38.4% identity in 797 aa overlap). Equivalent to AAK47741 from Mycobacterium tuberculosis strain CDC1551 (992 aa) but shorter 22 aa. Contains PS00523 Sulfatases signature 1 and PS01095 Chitinases family 18 active site signature. Belongs to the sulfatase family. O65931 No intermediary metabolism and respiration O65931 O65931 GO:0008152,GO:0004065 3.1.6.1 O65931 NC_000962.3 Mycobrowser_v5 CDS 3685983 3686900 . - 0 Rv3300c Rv3300c Function unknown Conserved hypothetical protein Rv3300c, (MTCI418A.02c), len: 305 aa. Conserved hypothetical protein, similar to various proteins (notably pseudoridine synthase family proteins) e.g. Q9RJ76|SCI41.08 putative ribosomal pseudouridine synthase from Streptomyces coelicolor (324 aa), FASTA scores: opt: 876, E(): 4.5e-48, (52.1% identity in 313 aa overlap); Q9I272|PA2043 hypothetical protein from Pseudomonas aeruginosa (300 aa), FASTA scores: opt: 676, E(): 1.8e-35, (42.55% identity in 268 aa overlap); Q9JZW8|NMB0867 YABO/YCEC/SFHB family protein from Neisseria meningitidis (serogroup B) (307 aa), FASTA scores: opt: 597, E(): 1.8e-30, (42.9% identity in 282 aa overlap); Q9JUY2|NMA1085 hypothetical protein from Neisseria meningitidis (serogroup A) (307 aa), FASTA scores: opt: 597, E(): 1.8e-30, (42.9% identity in 282 aa overlap); Q12362|RIB2_YEAST|RIB2|YOL066C DRAP deaminase (pseudouridine synthase family protein) from Saccharomyces cerevisiae (Baker's yeast) (591 aa), FASTA scores: opt: 338, E(): 6.9e-14, (32.95% identity in 246 aa overlap); Q9RTS2|DR1684 putative pseudouridine synthase from Deinococcus radiodurans (321 aa), FASTA scores: opt: 319, E(): 6.5e-13, (32.75% identity in 235 aa overlap); etc. Also similar to Mycobacterium tuberculosis hypothetical protein Q10786|Y04P_MYCTU|MTCY48.25c|Rv1540|MT1592 (308 aa) (28.8% identity in 299 aa overlap). O07166 No conserved hypotheticals O07166 O07166 GO:0009982,GO:0001522,GO:0003723 5.4.99.- O07166 NC_000962.3 Mycobrowser_v5 CDS 3686912 3687577 . - 0 Rv3301c phoY1 Involved in transcriptional regulation of active transport of inorganic phosphate across the membrane. Probable phosphate-transport system transcriptional regulatory protein PhoU homolog 1 PhoY1 Rv3301c, (MTCI418A.03c), len: 221 aa. Probable phoY1, phosphate-transport system regulatory protein, highly similar to Q50047|phoY|PHOU1|PHOY1|ML2188 phosphate transport system protein PHOU homolog 1 from Mycobacterium leprae (222 aa), FASTA scores: opt: 929, E(): 7.8e-51, (61.45% identity in 218 aa overlap). Also highly similar to Q9FCE2|2SCD46.42c putative regulatory protein (fragment) from Streptomyces coelicolor (123 aa), FASTA scores: opt: 324, E(): 1.8e-13, (43.65% identity in 103 aa overlap); Q9L0R3|SCD8A.01c putative phosphate transport system regulatory protein (fragment) from Streptomyces coelicolor (139 aa), FASTA scores: opt: 309, E(): 1.7e-12, (36.7% identity in 139 aa overlap); Q52989|PHOU_RHIME phosphate transport system protein from Rhizobium meliloti (Sinorhizobium meliloti) (237 aa), FASTA scores: opt: 292, E(): 3.1e-11, (26.3% identity in 213 aa overlap); etc. And highly similar to Mycobacterium tuberculosis O53833|PHU2_MYCTU|MTV043_13c|PHOU2|PHOY2|Rv0821c|MT0843 phosphate transport system protein PHOU homolog 2 (213 aa) (63.4% identity in 213 aa overlap). Belongs to the PHOU family. P9WI97 No cell wall and cell processes P65718 P9WI97 GO:0006817,GO:0005737 P9WI97 NC_000962.3 Mycobrowser_v5 CDS 3691639 3692808 . - 0 Rv3305c amiA1 Function unknown; certainly hydrolyses L-amino acid. Possible N-acyl-L-amino acid amidohydrolase AmiA1 (N-acyl-L-amino acid aminohydrolase) Rv3305c, (MTV016.04c), len: 389 aa. Possible amiA1, N-acyl-L-amino acid amidohydrolase (or peptidase), similar to many proteins e.g. Q9AK43|2SCK8.09 putative peptidase from Streptomyces coelicolor (410 aa), FASTA scores: opt: 1015, E(): 3.9e-54, (50.8% identity in 374 aa overlap); Q9UZ30|PAB0873 amino acid amidohydrolase from Pyrococcus abyssi (383 aa), FASTA scores: opt: 823, E(): 1.6e-42, (38.2% identity in 369 aa overlap); O58453|PH0722 long hypothetical amino acid amidohydrolase from Pyrococcus horikoshii (388 aa), FASTA scores: opt: 815, E(): 4.8e-42, (38.75% identity in 369 aa overlap); O34980|YTNL_BACSU hypothetical 45.2 KDA protein from B. subtilis (416 aa), FASTA scores: opt: 805, E(): 2.1e-41, (37.85% identity in 367 aa overlap); Q9KCF8|BH1613 N-acyl-L-amino acid amidohydrolase from Bacillus halodurans (404 aa), FASTA scores: opt: 795, E(): 8.1e-41, (37.7% identity in 382 aa overlap); BAB50445|MLR3583 hypothetical hippurate hydrolase from Rhizobium loti (Mesorhizobium loti) (387 aa), FASTA scores: opt: 761, E(): 8.9e-39, (37.65% identity in 385 aa overlap); Q9RXH4|DR0339 putative N-acyl-L-amino acid amidohydrolase from Deinococcus radiodurans (392 aa), FASTA scores: opt: 745, E(): 8.4e-38, (36.15% identity in 379 aa overlap); etc. Contains PS00639 Eukaryotic thiol (cysteine) proteases histidine active site. Note that previously known as amiA. L7N663 No intermediary metabolism and respiration Q7D5R0 L7N663 GO:0008152,GO:0005515,GO:0016787 3.5.1.- L7N663 NC_000962.3 Mycobrowser_v5 CDS 3721731 3722600 . - 0 Rv3335c Rv3335c Unknown Probable conserved integral membrane protein Rv3335c, (MTV016.35c), len: 289 aa. Probable conserved integral membrane protein, equivalent to Q49909|ML0687 putative membrane protein U0308AA from Mycobacterium leprae (313 aa), FASTA scores: opt: 1299, E(): 8.9e-75, (68.75% identity in 288 aa overlap). Also similar to other hypothetical bacterial proteins e.g. BAB37825|ECS4402 from Escherichia coli strain O157:H7 (alias P37642|YHJD_ECOLI|B3522 strain K12) (337 aa), FASTA scores: opt: 591, E(): 4.2e-30, (35.15% identity in 273 aa overlap); P45417|YHJD_ERWCH from Erwinia chrysanthemi (328 aa), FASTA scores: opt: 500, E(): 2.2e-24, (34.9% identity in 275 aa overlap); Q9KZA0|SC5G8.14 putative integral membrane protein from Streptomyces coelicolor (321 aa), FASTA scores: opt: 321, E(): 4.3e-13, (27.3% identity in 271 aa overlap); etc. O53385 No cell wall and cell processes O53385 O53385 GO:0004540 NC_000962.3 Mycobrowser_v5 CDS 3723656 3724042 . + 0 Rv3337 Rv3337 Function unknown Conserved hypothetical protein Rv3337, (MTV016.37), len: 128 aa. Conserved hypothetical protein, equivalent to N-terminus of Q49926|ML0685 TPEA (putative hydrolase) from Mycobacterium leprae (303 aa), FASTA scores: opt: 362, E(): 5.7e-17, (74.3% identity in 70 aa overlap). Also weak similarity in N-terminus to Q98JT7|BAB49078|MLR1789 probable epoxide hydrolase from Rhizobium loti (Mesorhizobium loti) (300 aa), FASTA scores: opt: 122, E(): 0.74, (31.95% identity in 97 aa overlap). Homology suggests this ORF should be in frame with the following ORF MTV016.38 but no sequence error could be found. Short distance to start of trpS suggests region may not be protein-coding. C-terminus extended since first submission (+47 aa). O53387 No conserved hypotheticals O53387 O53387 NC_000962.3 Mycobrowser_v5 CDS 3769111 3769500 . + 0 Rv3354 Rv3354 Function unknown Conserved hypothetical protein Rv3354, (MTV004.11), len: 129 aa. Conserved hypothetical protein, equivalent (but shorter 29 aa) to Q9CCM4|ML0676 hypothetical protein from Mycobacterium leprae (158 aa), FASTA scores: opt: 467, E(): 3.3e-21, (55.9% identity in 127 aa overlaps). Highly similar to O33192|LPRJ|Rv1690|MTCI125.12 hypothetical protein from Mycobacterium tuberculosis (127 aa), FASTA scores: opt: 329, E(): 4.7e-13, (46.95% identity in 115 aa overlap); and also similar to other Mycobacterium tuberculosis hypothetical proteins e.g. O07222|Rv1810|MTCY16F9.04c (118 aa), FASTA scores: opt: 195, E(): 4.2e-05, (37.15% identity in 113 aa overlap); MTCI125_11, MTCY16F9_4, MTV049_25. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O50383 No conserved hypotheticals O50383 O50383 NC_000962.3 Mycobrowser_v5 CDS 3694864 3696468 . + 0 Rv3308 pmmB Converts D-mannose 1-phosphate to D-mannose 6-phosphate. Probable phosphomannomutase PmmB (phosphomannose mutase) Rv3308, (MTV016.07), len: 534 aa. Probable pmmB, phosphomannomutase, equivalent to Q9CCL7|PMMB|ML0706 putative phospho-sugar mutase from Mycobacterium leprae (538 aa), FASTA scores: opt: 2681, E(): 1.4e-150, (76.95% identity in 538 aa overlap). Also similar to others e.g. Q9AD82|SCK13.08c from Streptomyces coelicolor (549 aa), FASTA scores: opt: 1378, E(): 8.9e-74, (46.7% identity in 529 aa overlap); Q9ZHL4|PMM (fragment so no homology at N-terminus for this one) from Haemophilus ducreyi (443 aa), FASTA scores: opt: 935, E(): 9.6e-48, (39.4% identity in 449 aa overlap); P18159|YHXB_BACSU from Bacillus subtilis (565 aa), FASTA scores: opt: 776, E(): 2.7e-38, (31.7% identity in 574 aa overlap); etc. Contains PS00710 Phosphoglucomutase and phosphomannomutase phosphoserine signature. Belongs to the phosphohexose mutases family. O53360 No intermediary metabolism and respiration O53360 O53360 GO:0000287,GO:0004615,GO:0005975 5.4.2.8 O53360 NC_000962.3 Mycobrowser_v5 CDS 3784932 3786272 . + 0 Rv3371 Rv3371 May be involved in synthesis of triacylglycerol Possible triacylglycerol synthase (diacylglycerol acyltransferase) Rv3371, (MTV004.29), len: 446 aa. Possible triacylglycerol synthase (See Daniel et al., 2004), similar to many Mycobacterium tuberculosis (strains H37Rv and CDC1551) hypothetical proteins e.g. O07035|YV30_MYCTU|Rv3130c|MTCY03A2.28|MTCY164.41c (463 aa), FASTA scores: opt: 556, E(): 7.7e-28, (44.95% identity in 447 aa overlap); MTY20B11_9, MTCY28_26, MTV013_8, MTCY21B4_43, MTCY493_29; etc. Also similar to O07692|MLCL383_9|MLCL383.18c hypothetical 14.1 KDA protein from Mycobacterium leprae (129 aa), FASTA scores: opt: 293, E(): 1.3e-11, (47.85% identity in 117 aa overlap). P9WKA9 No lipid metabolism O50400 P9WKA9 GO:0006071,GO:0008610,GO:0004144 2.3.1.20 P9WKA9 NC_000962.3 Mycobrowser_v5 CDS 3697198 3698097 . + 0 Rv3310 sapM Involved in cellular metabolism: acting on ester bonds [catalytic activity: an orthophosphoric monoester + H(2)O = an alcohol + orthophosphate]. Acid phosphatase (acid phosphomonoesterase) (phosphomonoesterase) (glycerophosphatase) Rv3310, (MTV016.09), sapM, len: 299 aa. Secreted acid phosphatase, with N-terminal sequence beginning with ASAL..., (see Saleh and Belisle, 2000). Similar to several fungal or bacterial acid phosphatases e.g. BAB50846|MLR4110 from Rhizobium loti (Mesorhizobium loti) (292 aa), FASTA scores: opt: 460, E(): 4.8e-22, (38.65% identity in 295 aa overlap); P34724|PHOA_ASPNG from Aspergillus niger (417 aa), FASTA scores: opt: 172, E(): 0.0013, (29.1% identity in 306 aa overlap); P08540|PHOX_KLULA from Kluyveromyces lactis (Yeast) (421 aa), FASTA scores: opt: 170, E(): 0.0018, (27.8% identity in 266 aa overlap); P37274|PHOA_PENCH from Penicillium chrysogenum (412 aa), FASTA scores: opt: 163, E(): 0.0049, (29.05% identity in 303 aa overlap); etc. O53361 No cell wall and cell processes O53361 O53361 GO:0008152,GO:0003993 3.1.3.2 O53361 NC_000962.3 Mycobrowser_v5 CDS 3719937 3720782 . - 0 Rv3333c Rv3333c Unknown Hypothetical proline rich protein Rv3333c, (MTV016.33c), len: 281 aa. Hypothetical unknown pro-rich protein. Equivalent to AAK47780 hypothetical protein from Mycobacterium tuberculosis strain CDC1551 (265 aa) but longer 16 aa. Predicted to be an outer membrane protein (See Song et al., 2008). O53383 No conserved hypotheticals O53383 O53383 O53383 NC_000962.3 Mycobrowser_v5 CDS 3699404 3700330 . - 0 Rv3312c Rv3312c Function unknown Conserved hypothetical protein Rv3312c, (MTV016.11), len: 308 aa. Hypothetical protein, similar to various proteins (principally hypothetical unknowns or hydrolases) e.g. Q9M9P2|T17B22.7 hypothetical protein from Arabidopsis thaliana (Mouse-ear cress) (326 aa), FASTA scores: opt: 261, E(): 2.6e-09, (27.55% identity in 323 aa overlap); Q9FWB6 putative alpha/beta hydrolase from Oryza sativa (Rice) (354 aa), FASTA scores: opt: 241, E(): 4.9e-08, (28.9% identity in 301 aa overlap) (note that Q9FWB6 correspond to Q9FWB5 putative alpha/beta hydrolase (353 aa) but longer 1 aa; and to Q9AUW9 hypothetical protein (332 aa) but longer 22 aa); Q9M382|F24B22.200 hypothetical protein from Arabidopsis thaliana (Mouse-ear cress) (342 aa), FASTA scores: opt: 222, E(): 8e-07, (27.6% identity in 319 aa overlap); Q9HWM9|PA4152 probable hydrolase from Pseudomonas aeruginosa (370 aa), FASTA scores: opt: 176, E(): 0.00071, (29.2% identity in 209 aa overlap); Q9L3R2 hydrolase from Rhizobium leguminosarum (261 aa), FASTA scores: opt: 174, E(): 0.00071, (28.9% identity in 173 aa overlap); P49323|PRXC_STRLI|CPO|CPOL non-heme chloroperoxidase from Streptomyces lividans (275 aa), FASTA scores: opt: 172, E(): 0.001, (30.9% identity in 194 aa overlap) (similarity only at N-terminus for this one); etc. Some similarity in N-terminal part to non-heme chloroperoxidases. Also similar to O05293|Rv1191|MTCI364.03 hypothetical protein from M. tuberculosis (304 aa), FASTA scores: opt: 417, E(): 3.1e-19, (32.6% identity in 279 aa overlap) (note that Rv1191 is equivalent to AAK45485 from Mycobacterium tuberculosis strain CDC1551 but shorter 14 aa, and that AAK45485 is annoted Hydrolase, alpha/beta hydrolase family). O53363 No conserved hypotheticals O53363 O53363 O53363 NC_000962.3 Mycobrowser_v5 CDS 3700705 3701016 . - 0 Rv3312A Rv3312A Unknown Secreted protein antigen Rv3312A, len: 103 aa. Secreted protein antigen, described in Corixa patent as having N-terminal sequence YYWCPGQPFDPAWGP. Equivalent to AAK47756 from Mycobacterium tuberculosis strain CDC1551 (114 aa) but shorter 11 aa. Q6MWY5 No cell wall and cell processes Q6MWY5 GO:0009405,GO:0009289,GO:0007155 NC_000962.3 Mycobrowser_v5 CDS 3701087 3702184 . - 0 Rv3313c add Catalyzes hydrolytic deamination of adenosine and generates inosine [catalytic activity: adenosine + H(2)O = inosine + NH(3) (also may act on deoxyadenosine)]. Probable adenosine deaminase Add (adenosine aminohydrolase) Rv3313c, (MTV016.13), len: 365 aa. Probable add, adenosine deaminase, equivalent to Q9CCL9|add|ML0700 putative adenosine deaminase from Mycobacterium leprae (362 aa), FASTA scores: opt: 2097, E(): 1.4e-127, (88.2% identity in 356 aa overlap). Also similar to many e.g. Q9AK25|2SCK8.27 from Streptomyces coelicolor (396 aa), FASTA scores: opt: 1578, E(): 3.7e-94, (66.65% identity in 360 aa overlap); Q17747|C06G3.5 from Caenorhabditis elegans (349 aa), FASTA scores: opt: 435, E(): 1.1e-20, (29.6% identity in 348 aa overlap); P22333|ADD_ECOLI|B1623 from Escherichia coli strain K12 (333 aa), FASTA scores: opt: 380, E(): 3.7e-17, (29.4% identity in 340 aa overlap); etc. Belongs to the adenosine and AMP deaminases family. P63907 No intermediary metabolism and respiration P63907 P63907 GO:0009168,GO:0004000 3.5.4.4 P63907 NC_000962.3 Mycobrowser_v5 CDS 3702184 3703467 . - 0 Rv3314c deoA The enzymes which catalyze the reversible phosphorylosis of pyrimidine nucleosides are involved in the degradation of these compounds and in their utilization as carbon and energy sources, or in the rescue of pyrimidine BASES for nucleotide synthesis [catalytic activity: thymidine + phosphate = thymine + 2-deoxy-D-ribose 1-phosphate]. Probable thymidine phosphorylase DeoA (tdrpase) (pyrimidine phosphorylase) Rv3314c, (MTV016.14), len: 427 aa. Probable deoA, thymidine phosporylase, highly similar to many e.g. Q9AK36|DEOA from Streptomyces coelicolor (427 aa), FASTA scores: opt: 1668, E(): 3.2e-90, (62.35% identity in 425 aa overlap); Q9CFM5|PDP from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (430 aa), FASTA scores: opt: 1031, E(): 5.5e-53, (46.45% identity in 392 aa overlap); P19971|TYPH_HUMAN|ECGF1 from Homo sapiens (Human) (482 aa), FASTA scores: opt: 957, E(): 1.3e-48, (44.45% identity in 441 aa overlap); P07650|TYPH_ECOLI|DEOA|TPP|TTG|B4382 from Escherichia coli strain K12 (440 aa), FASTA scores: opt: 847, E(): 3.2e-42, (41.55% identity in 438 aa overlap); etc. Contains PS00647 Thymidine and pyrimidine-nucleoside phosphorylases signature. Belongs to the thymidine/pyrimidine-nucleoside phosphorylases family. P9WFS1 No intermediary metabolism and respiration O53366 P9WFS1 GO:0006213,GO:0009032,GO:0006206 2.4.2.4 P9WFS1 NC_000962.3 Mycobrowser_v5 CDS 3704437 3704871 . + 0 Rv3317 sdhD Involved in tricarboxylic acid cycle. Putative hydrophobic component of the succinate dehydrogenase complex. Could be required to anchor the catalytic components to the cytoplasmic membrane Probable succinate dehydrogenase (hydrophobic membrane anchor subunit) SdhD (succinic dehydrogenase) (fumarate reductase) (fumarate dehydrogenase) (fumaric hydrogenase) Rv3317, (MTV016.17), len: 144 aa. Probable sdhD, membrane anchor of succinate dehydrogenase SdhD subunit , equivalent (but shorter 19 aa) to Q49915|SDHD|ML0698|L308_F1_25 putative succinate dehydrogenase hydrophobic membrane anchor protein from Mycobacterium leprae (163 aa), FASTA scores: opt: 878, E(): 1.9e-51, (85.2% identity in 142 aa overlap). Also similar to others e.g. Q9KZ89|SC5G8.25c from Streptomyces coelicolor (160 aa), FASTA scores: opt: 553, E(): 6.6e-30, (58.85% identity in 141 aa overlap); Q9RVR9|DR0953 from Deinococcus radiodurans (125 aa), FASTA scores: opt: 251, E(): 5.5e-10, (37.15% identity in 113 aa overlap); O29573|DHSD_ARCFU|SDHD|AF0684 from Archaeoglobus fulgidus (117 aa), FASTA scores: opt: 160, E(): 0.00056, (25.95% identity in 108 aa overlap); etc. Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, cytochrome B-556, and an hydrophobic anchor protein. O53369 No intermediary metabolism and respiration O53369 O53369 GO:0000104,GO:0055114 1.3.99.1 O53369 NC_000962.3 Mycobrowser_v5 CDS 3721257 3721697 . + 0 Rv3334 Rv3334 Involved in a transcriptional mechanism. Probable transcriptional regulatory protein (probably MerR-family) Rv3334, (MTV016.34), len: 146 aa. Probable transcriptional regulator, similar to many regulatory proteins (notably mercury resistance operon regulators) e.g. Q9HXV1|PA3689 probable transcriptional regulator MerR family from Pseudomonas aeruginosa (156 aa), FASTA scores: opt: 275, E(): 1.6e-11, (35.95% identity in 139 aa overlap); Q9AKR6|PBRR lead resistance operon regulator from Ralstonia metallidurans strain CH34 (plasmid pMOL30) (145 aa), FASTA scores: opt: 267, E(): 5.2e-11, (35.8% identity in 134 aa overlap); P95838|MERR mercuric resistance operon regulator from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (144 aa), FASTA scores: opt: 266, E(): 6e-11, (31.35% identity in 118 aa overlap); P22853|MERR_BACSR mercuric resistance operon regulator from Bacillus sp. strain RC607 (132 aa), FASTA scores: opt: 262, E(): 1e-10, (34.6% identity in 130 aa overlap); etc. Contains probable helix-turn-helix motif at aa 1-22 (Score 1478, +4.22 SD). Seems to belong to the MerR family of transcriptional regulators. O53384 No regulatory proteins O53384 O53384 GO:0006355,GO:0003700,GO:0000166 O53384 NC_000962.3 Mycobrowser_v5 CDS 3768222 3768593 . - 0 Rv3352c Rv3352c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv3352c, (MTV004.09c), len: 123 aa. Possible oxidoreductase, similar to part of several oxidoreductases (and hypothetical proteins) from diverse organisms e.g. Q9KYD6|SCD72A.20 putative lipoprotein (fragment) from Streptomyces coelicolor (403 aa), FASTA scores: opt: 348, E(): 7.9e-15, (51.0% identity in 102 aa overlap); BAB53081|MLR6875 probable oxidoreductase from Rhizobium loti (Mesorhizobium loti) (479 aa), FASTA scores: opt: 262, E(): 2.3e-09, (53.85% identity in 78 aa overlap); O94206|OX1 oxidoreductase from Claviceps purpurea (Ergot fungus) (483 aa), FASTA scores: opt: 245, E(): 2.7e-08, (42.6% identity in 115 aa overlap); Q9KHK2|ENCM putative FAD-dependent oxygenase ENCM from Streptomyces maritimus (464 aa), FASTA scores: opt: 238, E(): 7.2e-08, (43.95% identity in 91 aa overlap); etc. Also highly similar to part of O53608|Rv0063|MTV030.06 oxidoreductase (479 aa), FASTA scores: opt: 599, E(): 1.6e-30, (71.55% identity in 123 aa overlap); and to other Mycobacterium tuberculosis proteins e.g. Rv3353c and Rv3351c. All show similarity to a family of oxidoreductases in Mycobacterium tuberculosis, suggesting that frameshift mutations may have occurred. Sequence has been checked but no errors were found. O50381 No intermediary metabolism and respiration O50381 O50381 GO:0055114,GO:0016491,GO:0050660 1.-.-.- O50381 NC_000962.3 Mycobrowser_v5 CDS 3705000 3706772 . + 0 Rv3318 sdhA Involved in tricarboxylic acid cycle. Membrane-bound FAD-containing enzyme which is responsible for succinate interconversion [catalytic activity: succinate + acceptor = fumarate + reduced acceptor]. Probable succinate dehydrogenase (flavoprotein subunit) SdhA (succinic dehydrogenase) (fumarate reductase) (fumarate dehydrogenase) (fumaric hydrogenase) Rv3318, (MTV016.18), len: 590 aa. Probable sdhA, flavoprotein of succinate dehydrogenase SdhA subunit, equivalent to Q9CCM1|SDHA|ML0697 succinate dehydrogenase flavoprotein subunit from Mycobacterium leprae (584 aa), FASTA scores: opt: 3657, E(): 1.2e-217, (92.55% identity in 590 aa overlap). Also highly similar to others e.g. Q9KZ90|DHSA from Streptomyces coelicolor (584 aa), FASTA scores: opt: 2813, E(): 1.1e-165, (70.5% identity in 586 aa overlap); Q9RVS0|DR0952 from Deinococcus radiodurans (583 aa), FASTA scores: opt: 2203, E(): 4.1e-128, (57.35% identity in 593 aa overlap); P31038|DHSA_RICPR|SDHA|RP128 from Rickettsia prowazekii (596 aa), FASTA scores: opt: 1892, E(): 5.8e-109, (50.0% identity in 588 aa overlap); P10444|DHSA_ECOLI|SDHA|B0723|Z0877|ECS0748 from Escherichia coli strains K12 and O157:H7 (588 aa), FASTA scores: opt: 1844, E(): 5.2e-106, (48.75% identity in 591 aa overlap); etc. Contains PS00504 Fumarate reductase / succinate dehydrogenase FAD-binding site. Cofactor: FAD. Similar to the flavoprotein subunits of other species succinate dehydrogenase and of fumarate reductase. Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, cytochrome B-556, and an hydrophobic anchor protein. O53370 No intermediary metabolism and respiration O53370 O53370 GO:0009055,GO:0022900,GO:0000104,GO:0006099,GO:0050660 1.3.99.1 O53370 NC_000962.3 Mycobrowser_v5 CDS 3980659 3981048 . - 0 Rv3541c Rv3541c Function unknown Conserved protein Rv3541c, (MTCY03C7.15), len: 129 aa. Conserved protein, showing some similarity to Q9CBJ7|ML1909 hypothetical protein from Mycobacterium leprae (142 aa) FASTA scores: opt: 110, E(): 1.2, (27.95% identity in 118 aa overlap); and other (see also blastp results) e.g. Q9L0M3|SCD82.08 hypothetical 15.2 KDA protein from Streptomyces coelicolor (142 aa), FASTA scores: opt: 127, E(): 0.086, (27.65% identity in 123 aa overlap). Contains PS00075 Dihydrofolate reductase signature. I6XHI0 No conserved hypotheticals I6XHI0 I6XHI0 NC_000962.3 Mycobrowser_v5 CDS 3706772 3707563 . + 0 Rv3319 sdhB Involved in tricarboxylic acid cycle. Membrane-bound FAD-containing enzyme which is responsible for succinate interconversion [catalytic activity: succinate + acceptor = fumarate + reduced acceptor]. Probable succinate dehydrogenase (iron-sulphur protein subunit) SdhB (succinic dehydrogenase) (fumarate reductase) (fumarate dehydrogenase) (fumaric hydrogenase) Rv3319, (MTV016.19), len: 263 aa. Probable sdhB, iron-sulphur protein succinate dehydrogenase SdhB subunit , equivalent to Q49916|SDHB|ML0696|L308_F1_28 succinate dehydrogenase iron-sulfur protein from Mycobacterium leprae (264 aa), FASTA scores: opt: 1678, E(): 4.7e-99, (89.8% identity in 264 aa overlap). Also highly similar to other e.g. Q9KZ91|DHSB from Streptomyces coelicolor (257 aa), FASTA scores: opt: 1125, E(): 4.6e-64, (64.1% identity in 262 aa overlap); Q9RVS1|DR0951 from Deinococcus radiodurans (264 aa), FASTA scores: opt: 1014, E(): 5e-57, (57.25% identity in 255 aa overlap); Q9PEF5|XF1073 from Xylella fastidiosa (261 aa), FASTA scores: opt: 681, E(): 5.8e-36, (45.1% identity in 244 aa overlap); P07014|DHSB_ECOLI|SDHB|B0724 from Escherichia coli strain K12 (238 aa), FASTA scores: opt: 657, E(): 1.8e-34, (43.75% identity in 240 aa overlap); etc. Contains PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature. Cofactor: binds three different iron-sulfur clusters: a 2FE-2S, a 3FE-4S and a 4FE-4S. The iron-sulfur centers are similar to those of 'plant-type' 2FE-2S and 'bacterial-type' 4FE-4S ferredoxins. Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, cytochrome B-556, and an hydrophobic anchor protein. O53371 No intermediary metabolism and respiration O53371 GO:0051536,GO:0055114,GO:0000104,GO:0006099,GO:0009055 1.3.99.1 O53371 NC_000962.3 Mycobrowser_v5 CDS 3707642 3708070 . - 0 Rv3320c vapC44 Unknown Possible toxin VapC44. Contains PIN domain. Rv3320c, (MTV016.20c), len: 142 aa. Possible vapC44, toxin, part of toxin-antitoxin (TA) operon with Rv0300, contains PIN domain, see Arcus et al. 2005. Similar to several others in Mycobacterium tuberculosis (strains H37Rv and CDC1551) e.g. P95023|Rv2530c|MTCY159.26 (139 aa), FASTA scores: opt: 292, E(): 4.8e-14, (41.5% identity in 135 aa overlap); O53219|Rv2494|MTV008.50 (141 aa), FASTA scores: opt: 287, E(): 1.1e-13, (41.6% identity in 125 aa overlap); O07760|Rv0617|MTCY19H5.04c (133 aa), FASTA scores: opt: 252, E(): 3.3e-11, (37.8% identity in 127 aa overlap); etc. P9WF53 No virulence, detoxification, adaptation O53372 P9WF53 P9WF53 NC_000962.3 Mycobrowser_v5 CDS 3710245 3710379 . - 0 Rv3324A Rv3324A Thought to be involved in molybdopterin biosynthesis. Catalyzes the dehydratation of 4A-hydroxytetrahydropterins [catalytic activity: (6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4A-hydroxypterin = (6R)-6-(L-erythro-1,2- dihydroxypropyl)-7,8-dihydro-6H-pterin + H(2)O]. Probable fragment of pterin-4-alpha-carbinolamine dehydratase MOAB3 (PHS) (4-alpha-hydroxy-tetrahydropterin dehydratase) (pterin-4-a-carbinolamine dehydratase) (phenylalanine hydroxylase-stimulating protein) (PHS) (pterin carbinolamine dehydratase) (PCD) Rv3324A, len: 44 aa. Probable pseudogene moaB3, fragment of pterin-4-alpha-carbinolamine dehydratase, equivalent to C-terminus of MT3426|Q8VJ32 pterin-4-alpha-carbinolamine dehydratase from Mycobacterium tuberculosis strain CDC1551 (124 aa), FASTA scores: opt: 309, E(): 1.1e-20, (100.000% identity in 44 aa overlap), and C-terminus of Mb3354c|moaB3 probable pterin-4-alpha-carbinolamine dehydratase from Mycobacterium bovis (124 aa). Note that a deletion of DNA (RvD5 region) in Mycobacterium tuberculosis strain H37Rv resulted in a truncated CDS comparatively to Mycobacterium bovis or Mycobacterium tuberculosis strain CDC1551 genomes (see citations below). Yes intermediary metabolism and respiration NC_000962.3 Mycobrowser_v5 CDS 3710708 3711694 . + 0 Rv3326 Rv3326 Involved in the transposition of the insertion sequence element IS6110. Probable transposase Rv3326, (MTV016.26), len: 328 aa. Probable transposase for insertion element IS6110. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv3325 and Rv3326, the sequence UUUUAAAG (directly upstream of Rv3326) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 16 aa). P9WKH9 No insertion seqs and phages P0C5G8 P9WKH9 GO:0015074,GO:0006310,GO:0032196,GO:0003677 P9WKH9 NC_000962.3 Mycobrowser_v5 CDS 3832146 3833045 . - 0 Rv3413c Rv3413c Unknown Unknown alanine and proline rich protein Rv3413c, (MTCY78.16), len: 299 aa. Unknown ala-, pro-rich protein. P9WJ71 No conserved hypotheticals P9WJ71 GO:0016021 P9WJ71 NC_000962.3 Mycobrowser_v5 CDS 3709049 3709714 . - 0 Rv3323c moaX Thought to be involved in molybdenum cofactor biosynthesis. Probable MoaD-MoaE fusion protein MoaX Rv3323c, (MTV016.23c), len: 221 aa. Probable moaX, MoaD-MoaE fusion protein, similar (whole or partial) to several MoaD and MoaE proteins e.g. Q9RR88|DR2607 molybdenum cofactor biosynthesis protein D/E from Deinococcus radiodurans (229 aa), FASTA scores: opt: 407, E(): 1.8e-18, (32.75% identity in 223 aa overlap); Q9K8I7|MOAE|BH3019 molybdopterin converting factor (subunit 2) from Bacillus halodurans (156 aa), FASTA scores: opt: 375, E(): 1.3e-16, (41.65% identity in 132 aa overlap); O31705|MOAE molybdopterin converting factor (subunit 2) from Bacillus subtilis (157 aa), FASTA scores: opt: 368, E(): 3.6e-16, (41.65% identity in 132 aa overlap); etc. C-terminus highly similar to O05795|MOAE_MYCTU|Rv3119|MT3201|MTCY164.29|MOAE1 putative molybdenum cofactor biosynthesis protein E from Mycobacterium tuberculosis (147 aa), FASTA scores: opt: 733, E(): 5.4e-39, (76.2% identity in 143 aa overlap); and N-terminus highly similar to O05789|MOAD1|Rv3112|MTCY164.22 putative molybdenum cofactor biosynthesis protein D from Mycobacterium tuberculosis (83 aa), FASTA scores: opt: 333, E(): 3.2e-14, (65.05% identity in 83 aa overlap). Q6MWY3 No intermediary metabolism and respiration Q6MWY3 Q6MWY3 GO:0006790,GO:0006777 Q6MWY3 NC_000962.3 Mycobrowser_v5 CDS 3714392 3715708 . + 0 Rv3329 Rv3329 Function unknown; probably involved in cellular metabolism. Probable aminotransferase Rv3329, (MTV016.29), len: 438 aa (start uncertain). Probable aminotransferase, similar to many e.g. O86744|SC6A9.12 from Streptomyces coelicolor (457 aa), FASTA scores: opt: 2120, E(): 5.1e-125, (70.1% identity in 438 aa overlap); Q9I6J2|PA0299 from Pseudomonas aeruginosa (456 aa), FASTA scores: opt: 983, E(): 5.7e-54, (38.1% identity in 425 aa overlap); Q53196|Y4UB_RHISN from Rhizobium sp. strain NGR234 plasmid sym pNGR234a (467 aa), FASTA scores: opt: 971, E(): 3.3e-53, (39.25% identity in 438 aa overlap); P33189|YHXA_BACSU from Bacillus subtilis (450 aa), FASTA scores: opt: 933, E(): 7.5e-51, (40.25% identity in 435 aa overlap); etc. Equivalent to AAK47775 from Mycobacterium tuberculosis strain CDC1551 (466 aa) but shorter 28 aa. Cofactor: pyridoxal phosphate. Could belong to class-III of pyridoxal-phosphate-dependent aminotransferases. O53379 No intermediary metabolism and respiration O53379 O53379 GO:0008483,GO:0030170 2.6.1.- O53379 NC_000962.3 Mycobrowser_v5 CDS 3755952 3767102 . - 0 Rv3350c PPE56 Function unknown PPE family protein PPE56 Rv3350c, (MTV004.07c), len: 3716 aa. PPE56, Member of the Mycobacterium tuberculosis PPE family of Gly-, Ala-, Asn-rich proteins, similar to many Mycobacterium tuberculosis proteins from strains H37Rv and CDC1551, e.g. O50378|Rv3347c|MTV004.03c (3157 aa), FASTA scores: opt: 6497, E(): 0, (61.65% identity in 3756 aa overlap); MTCY28_16, MTV050_2, MTY13E10_17, MTCY63_10, MTCY180_1, MTCY63_9, MTV050_1, MTV014_3, MTY13E10_15; etc. Q6MWX8 No PE/PPE Q6MWX8 Q6MWX8 Q6MWX8 NC_000962.3 Mycobrowser_v5 CDS 3717090 3718598 . + 0 Rv3331 sugI Thought to be involved in transport of sugar across the membrane. Responsible for the translocation of the substrate across the membrane. Probable sugar-transport integral membrane protein SugI Rv3331, (MTV016.31), len: 502 aa (start uncertain). Probable sugI, sugar-transport integral membrane protein, possibly member of major facilitator superfamily (MFS), similar to several transporters e.g. P37021|GALP_ECOLI|B2943 galactose-proton symporter (galactose transporter) from Escherichia coli strain K12 (464 aa), FASTA scores: opt: 818, E(): 1.8e-39, (31.85% identity in 446 aa overlap); P96742|YWTG metabolite-transport-related protein from Bacillus subtilis (457 aa), FASTA scores: opt: 810, E(): 5e-39, (33.2% identity in 428 aa overlap); AAG58074|GALP (alias BAB37242|ECS3819) galactose-proton symport of transport system from Escherichia coli strain O157:H7 EDL933 (464 aa), FASTA scores: opt: 810, E(): 5.1e-39, (32.2% identity in 432 aa overlap); P46333|CSBC_BACSU|SS92BR probable metabolite transport protein from Bacillus subtilis (461 aa), FASTA scores: opt: 792, E(): 5.4e-38, (33.7% identity in 442 aa overlap); etc. Equivalent to AAK47777|MT343 from Mycobacterium tuberculosis strain CDC1551 (500 aa) but with some divergence between residues 229 and 254. Contains PS00216 Sugar transport proteins signature 1 and PS00217 Sugar transport proteins signature 2. Belongs to the sugar transporter family. L0TDU1 No cell wall and cell processes O53381 L0TDU1 GO:0022891,GO:0055085,GO:0016021 L0TDU1 NC_000962.3 Mycobrowser_v5 CDS 3718595 3719746 . + 0 Rv3332 nagA Involved in N-acetyl glucosamine utilization pathway [catalytic activity: N-acetyl-D-glucosamine 6-phosphate + H(2)O = D-glucosamine 6-phosphate + acetate]. Probable N-acetylglucosamine-6-phosphate deacetylase NagA (GlcNAc 6-P deacetylase) Rv3332, (MTV016.32), len: 383 aa. Probable nagA, N-acetylglucosamine-6-phosphate deacetylase, similar to many e.g. Q9KXV7|SCD95A.17c putative deacetylase from Streptomyces coelicolor (381 aa), FASTA scores: opt: 1090, E(): 1.6e-55, (47.8% identity in 385 aa overlap); Q9PDB4|XF1465 N-acetylglucosamine-6-phosphate deacetylase from Xylella fastidiosa (386 aa), FASTA scores: opt: 667, E(): 3.5e-31, (38.3% identity in 394 aa overlap); Q9AAZ9|CC0443 N-acetylglucosamine-6-phosphate deacetylase from Caulobacter crescentus (378 aa), FASTA scores: opt: 661, E(): 7.5e-31, (38.9% identity in 383 aa overlap); O34450||NAGA_BACSU N-acetylglucosamine-6-phosphate deacetylase from Bacillus subtilis (396 aa), FASTA scores: opt: 571, E(): 1.2e-25, (32.45% identity in 376 aa overlap); etc. Equivalent to AAK47778 from Mycobacterium tuberculosis strain CDC1551 (346 aa) but longer 37 aa. Belongs to the NagA family. O53382 No cell wall and cell processes O53382 O53382 GO:0008448,GO:0006044 3.5.1.25 O53382 NC_000962.3 Mycobrowser_v5 CDS 3729364 3736935 . - 0 Rv3343c PPE54 Function unknown PPE family protein PPE54 Rv3343c, (MTV016.43c), len: 2523 aa. PPE54, Member of the Mycobacterium tuberculosis PPE family, MPTR subgroup of Gly-, Asn-rich proteins. Most similar to O50379|Rv3350c|MTV004.07c|MTV004_5 from Mycobacterium tuberculosis strain H37Rv (3716 aa), FASTA scores: opt: 4672, E(): 4e-211, (44.2% identity in 3174 aa overlap); and also similar to MTV004_3, MTCY63_9, MTY13E10_17, MTY13E10_16, MTCY180_1, MTV050_1, MTCY3C7_23, MTV014_3, MTCY63_10; etc. Q6MWY2 No PE/PPE Q6MWY2 Q6MWY2 Q6MWY2 NC_000962.3 Mycobrowser_v5 CDS 3722621 3723631 . - 0 Rv3336c trpS Involved in translation mechanism [catalytic activity: ATP + L-tryptophan + tRNA(TRP) = AMP + pyrophosphate + L-tryptophanyl-tRNA(TRP)]. Probable tryptophanyl-tRNA synthetase TrpS (tryptophan--tRNA ligase) (TRPRS) (tryptophan translase) Rv3336c, (MTV016.36c), len: 336 aa. Probable trpS, tryptophanyl-tRNA synthetase, equivalent to Q49901|SYW_MYCLE|TRPS|ML0686|L308_C1_147 tryptophanyl-tRNA synthetase from Mycobacterium leprae (343 aa), FASTA scores: opt: 1859, E(): 4.8e-107, (83.75% identity in 339 aa overlap). Also similar to many e.g. Q9KZA7|TRPS2 from Streptomyces coelicolor (339 aa), FASTA scores: opt: 1359, E(): 2.6e-76, (60.3% identity in 335 aa overlap); Q9EYY6|TRPS from Klebsiella aerogenes (334 aa), FASTA scores: opt: 1077, E(): 5.5e-59, (52.15% identity in 328 aa overlap); P00954|SYW_ECOLI|TRPS|B3384 from Escherichia coli strain K12 (334 aa), FASTA scores: opt: 1074, E(): 8.3e-59, (51.85% identity in 328 aa overlap); etc. Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Belongs to class-I aminoacyl-tRNA synthetase family. P9WFT3 No information pathways P67590 P9WFT3 GO:0005737,GO:0004830,GO:0006436,GO:0005524 6.1.1.2 P9WFT3 NC_000962.3 Mycobrowser_v5 CDS 4199721 4200221 . - 0 Rv3753c Rv3753c Function unknown Conserved protein Rv3753c, (MTV025.101c), len: 166 aa. Conserved protein, only equivalent to Q9CB33|ML2473 hypothetical protein from Mycobacterium leprae (159 aa) FASTA scores: opt: 920 E(): 1.4e-52,, (88.6% identity in 158 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O69720 No conserved hypotheticals O69720 NC_000962.3 Mycobrowser_v5 CDS 3723904 3724548 . + 0 Rv3338 Rv3338 Function unknown Conserved hypothetical protein Rv3338, (MTV016.38), len: 214 aa. Hypothetical protein, equivalent to C-termini of Q49926|ML0685 TPEA (putative hydrolase) from Mycobacterium leprae (303 aa), FASTA scores: opt: 984, E(): 2.6e-56, (65.4% identity in 214 aa overlap); and O32873|MLCB1779.02 hypothetical 31.8 KDA protein (similar to alpha/beta hydrolase fold) from Mycobacterium leprae (292 aa), FASTA scores: opt: 984, E(): 2.5e-56, (65.4% identity in 214 aa overlap). Also similar to C-termini of several hypothetical proteins (generally hydrolases) e.g. Q9K3H6|2SCG18.11 putative hydrolase from Streptomyces coelicolor (316 aa), FASTA scores: opt: 213, E(): 1.4e-06, (29.75% identity in 185 aa overlap). Homology suggests that this ORF should be in frame with the previous ORF MTV016.37 but no sequence error could be found. O53388 No conserved hypotheticals O53388 O53388 NC_000962.3 Mycobrowser_v5 CDS 3768736 3768996 . - 0 Rv3353c Rv3353c Function unknown Conserved hypothetical protein Rv3353c, (MTV004.10c), len: 86 aa. Hypothetical protein, showing some similarity to Q9X5Q4|MITR MITR protein from Streptomyces lavendulae (514 aa), FASTA scores: opt: 134, E(): 0.09, (29.5% identity in 78 aa overlap); and weak to Q49720|B1549_C3_218 from Mycobacterium leprae (222 aa), FASTA scores: opt: 99, E(): 8.8, (32.9% identity in 76 aa overlap). But highly similar to N-terminal part of O53608|Rv0063|MTV030.06 oxidoreductase from Mycobacterium tuberculosis (479 aa), FASTA scores: opt: 305, E(): 4.9e-13, (52.9% identity in 87 aa overlap); and some similarity can be found with Rv3352c and Rv3351c. All show similarity to a family of oxidoreductases in Mycobacterium tuberculosis, suggesting that frameshift mutations may have occurred. Sequence has been checked but no errors were found. Start changed since original submission. O50382 No conserved hypotheticals O50382 NC_000962.3 Mycobrowser_v5 CDS 3727488 3728627 . + 0 Rv3341 metA Catalyzes acylation of L-homoserine. Involved in biosynthesis of methionine; HTA variant; first step [catalytic activity: acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine]. Probable homoserine O-acetyltransferase MetA (homoserine O-trans-acetylase) (homoserine transacetylase) (HTA) Rv3341, (MTV016.41), len: 379 aa. Probable metA, homoserine o-acetyltransferase (see citation below), equivalent to O32874|METX_MYCLE|meta|ML0682|MLCB1779.11 homoserine O-acetyltransferase from Mycobacterium leprae (382 aa), FASTA scores: opt: 2263, E(): 9.2e-129, (85.0% identity in 380 aa overlap). Also highly similar to many e.g. O68640|METX_CORGL|meta from Corynebacterium glutamicum (Brevibacterium flavum) (379 aa), FASTA scores: opt: 1135, E(): 5.9e-61, (48.5% identity in 371 aa overlap); Q9AAS1|CC0525 from Caulobacter crescentus (382 aa), FASTA scores: opt: 860, E(): 2e-44, (40.5% identity in 363 aa overlap); P94891|METX_LEPME from Leptospira meyeri (379 aa), FASTA scores: opt: 787, E(): 4.9e-40, (38.2% identity in 385 aa overlap); etc. Belongs to the ab hydrolase family, HTA subfamily. P9WJY9 No intermediary metabolism and respiration P0A5J8 P9WJY9 GO:0004414,GO:0009086,GO:0005737 2.3.1.31 P9WJY9 NC_000962.3 Mycobrowser_v5 CDS 3839691 3840197 . - 0 Rv3422c Rv3422c Function unknown Conserved hypothetical protein Rv3422c, (MTCY78.07), len: 168 aa. Conserved hypothetical protein, equivalent to Q49864|YY22_MYCLE|ML0377|U229F|B229_C2_205 hypothetical 17.6 KDA protein from Mycobacterium leprae (161 aa), FASTA scores: opt: 752, E(): 8.3e-38, (77.4% identity in 146 aa overlap). Also similar to other hypothetical bacterial proteins e.g. O86788|YJEE_STRCO|SC6G4.25 from Streptomyces coelicolor (148 aa), FASTA scores: opt: 377, E(): 1.2e-15, (50.85% identity in 120 aa overlap); Q9X1W7|TM1632 from Thermotoga maritima (161 aa), FASTA scores: opt: 247, E(): 6.2e-08, (39.4% identity in 137 aa overlap); Q9RRY1|DR2351 from Deinococcus radiodurans (148 aa), FASTA scores: opt: 236, E(): 2.6e-07, (38.6% identity in 127 aa overlap); etc. Contains PS00017 ATP /GTP-binding site motif A. P9WFS7 No conserved hypotheticals P67171 P9WFS7 GO:0005524 P9WFS7 NC_000962.3 Mycobrowser_v5 CDS 3738158 3742774 . - 0 Rv3345c PE_PGRS50 Function unknown PE-PGRS family protein PE_PGRS50 Rv3345c, (MTV004.01c-MTV016.45c), len: 1538 aa. PE_PGRS50, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002). Similar to AAK47791 from strain CDC1551 but with some big gaps (after residues 501 and 1419; and for AAK47791 after residue 991). Similar to many from Mycobacterium tuberculosis strains H37Rv and CDC1551. Q6MWY0 No PE/PPE Q6MWY0 Q6MWY0 Q6MWY0 NC_000962.3 Mycobrowser_v5 CDS 3743198 3743455 . - 0 Rv3346c Rv3346c Function unknown Conserved transmembrane protein Rv3346c, (MTV004.02c), len: 85 aa. Conserved transmembrane protein, highly similar to mycobacterium hypothetical proteins O50384|Rv3355c|MTV004.12c from strain H37Rv (97 aa), FASTA scores: opt: 413, E(): 4.6e-23, (85.55% identity in 97 aa overlap); O32878|MLCB1779.16c|ML0675 from Mycobacterium leprae (91 aa), FASTA scores: opt: 349, E(): 1.7e-18, (67.35% identity in 95 aa overlap). Contains possible membrane spanning regions. O50377 No cell wall and cell processes O50377 O50377 NC_000962.3 Mycobrowser_v5 CDS 3770773 3771048 . + 0 Rv3357 relJ Function unknown Antitoxin RelJ Rv3357, (MTV004.14), len: 91 aa. RelJ, antitoxin, part of toxin-antitoxin (TA) operon with Rv3358 (See Cherny et al., 2004; Pandey and Gerdes, 2005), highly similar to other hypothetical proteins e.g. Q9Z4V7|YU1E_STRCO (alias CAC37261|SCBAC17D6.02) ORFU1E (belongs to the PHD/YEFM family) from Streptomyces coelicolor (87 aa), FASTA scores: opt: 344, E(): 1.9e-17, (62.05% identity in 87 aa overlap); P46147|YEFM_ECOLI|B2017 from Escherichia coli strain K12 (83 aa), FASTA scores: opt: 215, E(): 1.6e-08, (50.0% identity in 72 aa overlap); BAB58570|SAV2408 from Staphylococcus aureus subsp. aureus Mu50 (83 aa), FASTA scores: opt: 161, E(): 8.8e-05, (39.95% identity in 77 aa overlap); Q9Z5W8 putative PHD protein from Francisella novicid (85 aa), FASTA scores: opt: 143, E(): 0.0016, (28.9% identity in 83 aa overlap); etc. Also similar to Rv1247c|MTV006.19c (89 aa) (36.9% identity in 84 aa overlap). Seems to belong to the PHD/YEFM family. P9WF25 No virulence, detoxification, adaptation 3CTO,3D55,3OEI P65067 P9WF25 P9WF25 NC_000962.3 Mycobrowser_v5 CDS 3771045 3771302 . + 0 Rv3358 relK Function unknown Toxin RelK Rv3358, (MTV004.15), len: 85 aa. RelK, toxin, part of toxin-antitoxin (TA) operon with Rv3357 (See Cherny et al., 2004; Pandey and Gerdes, 2005), highly similar to other hypohetical proteins e.g. Q9Z4V8|SCBAC17D6.03 from Streptomyces coelicolor (84 aa), FASTA scores: opt: 393, E(): 1.1e-21, (59.75% identity in 82 aa overlap); P56605|YOEB_ECOLI from Escherichia coli (84 aa), FASTA scores: opt: 305, E(): 2.2e-15, (49.35% identity in 77 aa overlap); Q9Z5W7 putative doc protein from Francisella novicida (68 aa), FASTA scores: opt: 253, E(): 9.6e-12, (51.6% identity in 62 aa overlap); BAB58569|SAV2407 from Staphylococcus aureus subsp. aureus Mu50 (88 aa), FASTA scores: opt: 250, E(): 2e-11, (40.5% identity in 84 aa overlap); etc. P9WF09 No virulence, detoxification, adaptation 3OEI P64528 P9WF09 GO:0004519,GO:0006401 3.1.-.- P9WF09 NC_000962.3 Mycobrowser_v5 CDS 3772651 3773019 . + 0 Rv3360 Rv3360 Function unknown Conserved hypothetical protein Rv3360, (MTV004.17), len: 122 aa. Hypothetical protein, highly similar to the N-terminus of O65934|Rv1747|MTCY28.10|MTCY04C12.31 probable ABC-transporter ATP-binding protein from Mycobacterium tuberculosis (865 aa), FASTA scores: opt: 480, E(): 4.7e-25, (61.0% identity in 118 aa overlap); and some similarity with the N-terminus of P96214|Rv3863|MTCY01A6.05c hypothetical 41.1 KDA protein from Mycobacterium tuberculosis (392 aa), FASTA scores: opt: 138, E(): 0.033, (31.95% identity in 97 aa overlap). Some weak similarity with the N-terminus of other hypothetical proteins e.g. P73823|CYAA|SLR1991 adenylate cyclase from Synechocystis sp. strain PCC 6803 (337 aa), FASTA scores: opt: 127, E(): 0.16, (28.55% identity in 112 aa overlap). O50389 No conserved hypotheticals O50389 O50389 NC_000962.3 Mycobrowser_v5 CDS 3773016 3773567 . - 0 Rv3361c Rv3361c Function unknown Conserved protein Rv3361c, (MTV004.18c), len: 183 aa. Conserved protein, with some similarity to various proteins e.g. P74221|YB52_SYNY3|SLR1152 hypothetical 36.2 KDA protein SLR (contains 5 pentapeptide repeat domains) from Synechocystis sp. strain PCC 6803 (331 aa), FASTA scores: opt: 252, E(): 3.9e-10, (30.55% identity in 167 aa overlap); Q9SE95 FH protein interacting protein FIP2 from Arabidopsis thaliana (Mouse-ear cress) (298 aa), FASTA scores: opt: 207, E(): 4.4e-07, (30.35% identity in 168 aa overlap); Q9A735|CC1891 pentapeptide repeat family protein from Caulobacter crescentus (250 aa), FASTA scores: opt: 181, E(): 2.3e-05, (24.05% identity in 187 aa overlap); etc. I6YBX3 No conserved hypotheticals 2BM4,2BM5,2BM6,2BM7 O50390 I6YBX3 I6YBX3 NC_000962.3 Mycobrowser_v5 CDS 3774871 3777501 . - 0 Rv3365c Rv3365c Function unknown Conserved protein Rv3365c, (MTV004.22c), len: 876 aa. Conserved protein, similar to various proteins from Streptomyces coelicolor e.g. O86525|SC1C2.25c hypothetical 139.7 KDA protein (similar to other prokaryotic sensory transduction histidine kinases) (1329 aa), FASTA scores: opt: 879, E(): 5.4e-32, (29.9% identity in 924 aa overlap) (similarity in N-terminal part for this one); O86522|SC1C2.21c hypothetical 119.9 KDA protein (similar to other prokaryotic sensory transduction histidine kinases) (1111 aa), FASTA scores: opt: 855, E(): 5.6e-31, (28.9% identity in 892 aa overlap) (similarity in N-terminal part for this one); Q9KZI5|SCG8A.14c putative membrane protein (862 aa), FASTA scores: opt: 791, E(): 3.3e-28, (30.8% identity in 828 aa overlap); Q9KZN0|SC1A8A.22c (943 aa), FASTA scores: opt: 660, E(): 2.5e-22, (27.65% identity in 893 aa overlap); etc. Similar in part to two consecutive Mycobacterium leprae hypothetical ORFs, probably representing a pseudogene: O07701|MLCL383.27 (118 aa), FASTA scores: opt: 430, E(): 1e-12, (58.25% identity in 115 aa overlap); and O07700|MLCL383.26 (111 aa), FASTA scores: opt: 271, E(): 1.3e-05, (50.4% identity in 121 aa overlap). Contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. Q93IG6 No conserved hypotheticals Q93IG6 Q93IG6 GO:0016021,GO:0016301,GO:0004871,GO:0007165,GO:0000160,GO:0005524 Q93IG6 NC_000962.3 Mycobrowser_v5 CDS 3788621 3790048 . + 0 Rv3375 amiD Involved in cellular metabolism [catalytic activity: a monocarboxylic acid amide + H(2)O = a monocarboxylate + NH(3)]. Probable amidase AmiD (acylamidase) (acylase) Rv3375, (MTV004.33), len: 475 aa. Probable amiD, amidase, similar to various amidases e.g. Q53116|AMDA enantiomerase-selective amidase from Rhodococcus sp. (462 aa), FASTA scores: opt: 1036, E(): 1.6e-54, (38.6% identity in 464 aa overlap); Q9ZHK8|PZAA nicotinamidase/pyrazinamidase from Mycobacterium smegmatis (468 aa), FASTA scores: opt: 930, E(): 3.4e-48, (36.3% identity in 463 aa overlap); Q9A551|CC2613 pyrazinamidase/nicotinamidase from Caulobacter crescentus (464 aa), FASTA scores: opt: 841, E(): 7.1e-43, (39.45% identity in 469 aa overlap); O69768|AMID_PSEPU amidase from Pseudomonas putida (466 aa), FASTA scores: opt: 800, E(): 2e-40, (33.6% identity in 467 aa overlap); O28325|YJ54_ARCFU|AF1954 putative amidase from Archaeoglobus fulgidu (453 aa), FASTA scores: opt: 669, E(): 1.3e-32, (30.4% identity in 467 aa overlap); etc. Also some similarity to AMIB2|Rv1263|MT1301|MTCY50.19c putative amidase from Mycobacterium tuberculosis (462 aa), (31.5% identity in 466 aa overlap). Seems belong to the amidase family. P9WQ93 No intermediary metabolism and respiration P63496 P9WQ93 GO:0016884,GO:0004040 3.5.1.4 P9WQ93 NC_000962.3 Mycobrowser_v5 CDS 3778568 3780334 . + 0 Rv3367 PE_PGRS51 Function unknown PE-PGRS family protein PE_PGRS51 Rv3367, (MTV004.25), len: 588 aa. PE_PGRS51, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002). Similar to many from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. O50415|Rv3388|MTV004.46 (731 aa), FASTA scores: opt: 1999, E(): 7.2e-72, (55.0% identity in 620 aa overlap); and MTV004_44, MTV043_65, MTV006_15, MTCY63_2, MTCY21B4_13, MTV023_21, MTV008_43, MTCY24A1_4, MTV023_15; etc. Equivalent to AAK47814 from Mycobacterium tuberculosis strain CDC1551 (628 aa) but shorter 37 aa. L0TCB8 No PE/PPE Q6MWX7 L0TCB8 L0TCB8 NC_000962.3 Mycobrowser_v5 CDS 3780335 3780979 . - 0 Rv3368c Rv3368c Function unknown; probably involved in cellular metabolism Possible oxidoreductase Rv3368c, (MTV004.26c), len: 214 aa. Possible oxidoreductase, equivalent to O07697|MLCL383.23|ML0418 hypothetical 23.6 KDA protein (putative oxidoreductase) from Mycobacterium leprae (210 aa), FASTA scores: opt: 1215, E(): 1.5e-74, (81.4% identity in 210 aa overlap). Also similar to O30106|AF0131 putative NAD(P)H-flavin oxidoreductase from Archaeoglobus fulgidus (194 aa), FASTA scores: opt: 139, E(): 0.028, (29.0% identity in 207 aa overlap); Q60049|NOX_THETH NADH dehydrogenase from Thermus aquaticus (subsp. thermophilus) (205 aa), FASTA scores: opt: 169, E(): 0.00028, (28.3% identity in 212 aa overlap); and shows some similarity to other hypothetical proteins (unknowns or oxidoreductases). O50397 No intermediary metabolism and respiration O50397 O50397 GO:0016491,GO:0055114 1.-.-.- O50397 NC_000962.3 Mycobrowser_v5 CDS 3780978 3781412 . + 0 Rv3369 Rv3369 Function unknown Conserved protein Rv3369, (MTV004.27), len: 144 aa. Conserved protein. C-terminus is similar to N-terminus of O07696|MLCL383.22c hypothetical 14.7 KDA protein from Mycobacterium leprae (131 aa), FASTA scores: opt: 174, E(): 6e-05, (67.55% identity in 37 aa overlap). Also some slight similarity to Q9EWU1|3SC5B7.08c from Streptomyces coelicolor (153 aa), FASTA scores: opt: 125, E(): 0.13, (31.05% identity in 116 aa overlap). O50398 No conserved hypotheticals O50398 O50398 GO:0010181 O50398 NC_000962.3 Mycobrowser_v5 CDS 3797437 3798489 . - 0 Rv3383c idsB Involved in biosynthesis of membrane ether-linked lipids. Catalyzes the trans-addition of the three molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate which is a precursor of the ether-linked lipids. Catalyze the consecutive condensation of homoallylic diphosphate of isopentenyl diphosphates (IPP, C5) with allylic diphosphates to synthesize prenyl diphosphates of various chain lengths. Possible polyprenyl synthetase IdsB (polyprenyl transferase) (polyprenyl diphosphate synthase) Rv3383c, (MTV004.41c), len: 350 aa. Possible idsB, polyprenyl transferase (polyprenyl diphosphate synthase) , similar to many prenyltransferases involved in lipid biosynthesis e.g. Q9RGW1|GTR geranyl transferase from Streptomyces coelicolor (386 aa), FASTA scores: opt: 908, E(): 3.7e-50, (48.8/% identity in 334 aa overlap); Q9KWG0|GGDPS geranyl geranyl diphosphate synthase from Kitasatospora griseola (Streptomyces griseolosporeus) (348 aa), FASTA scores: opt: 801, E(): 2e-43, (41.5% identity in 347 aa overlap); Q9X7V8|SC6A5.12 putative polyprenyl synthetase from Streptomyces coelicolor (378 aa), FASTA scores: opt: 779, E(): 5.3e-42, (44.45% identity in 324 aa overlap); Q9S5E9 farnesyl, geranylgeranyl, geranylfarnesyl, hexaprenyl, heptaprenyl diphosphate synthase (self-HEPPS) from Synechococcus elongatus (324 aa), FASTA scores: opt: 563, E(): 2.3e-28, (39.85% identity in 241 aa overlap) (see citation below); O26156|IDSA_METTH|MTH50 bifunctional short chain isoprenyl diphosphate synthase [includes: farnesyl pyrophosphate synthetase (FPP synthetase) (dimethylallyltransferase) and geranyltranstransferase] from Methanobacterium thermoautotrophicum (325 aa), FASTA scores: opt: 540, E(): 6.5e-27, (35.75% identity in 319 aa overlap); P95999|GGPP_SULSO|GDS|GDS-1|SSO0061|C05010|C05_049 geranylgeranyl pyrophosphate synthetase (GGPP synthetase) (GGPS) [includes: dimethylallyltransferase and geranyltranstransferase and farnesyltranstransferase] from Sulfolobus solfataricus (332 aa), FASTA scores: opt: 511, E(): 4.5e-25 (36.9% identity in 244 aa overlap); etc. Also similar to Q50727|GGPP_MYCTU|Rv3398c|MT3506|MTCY78.30 probable multifunctional geranylgeranyl pyrophosphate synthetase [includes: dimethylallyltransferase; geranyltranstransferase; farnesyltranstransferase] from Mycobacterium tuberculosis (359 aa), FASTA scores: opt: 687, E(): 3.4e-36, (39.1% identity in 325 aa overlap). Contains PS00723 Polyprenyl synthetases signature 1. Belongs to the FPP/GGPP synthetases family. O50410 No lipid metabolism O50410 O50410 GO:0016740,GO:0008299 2.5.1.- O50410 NC_000962.3 Mycobrowser_v5 CDS 3788368 3788616 . + 0 Rv3374 echA18.1 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Probable enoyl-CoA hydratase (fragment) EchA18.1 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv3374, (MTV004.32), len: 82 aa. Probable echA18.1, enoyl-CoA hydratase C-terminus, similar to the C-terminus of several enoyl-CoA hydratases e.g. Q9I5I4|PA0745 from Pseudomonas aeruginosa (272 aa), FASTA scores: opt: 123, E(): 0.13, (34.55% identity in 81 aa overlap); P97087|CRT from Clostridium thermosaccharolyticum (Thermoanaerobacterium thermosaccharolyticum) (259 aa), FASTA scores: opt: 115, E(): 0.45, (32.95% identity in 82 aa overlap); Q9I002|PA2841 from Pseudomonas aeruginosa (263 aa), FASTA scores: opt: 108, E(): 1.4, (30.95% identity in 84 aa overlap); etc. Also some similarity to C-terminus of O29956|AF0285 3-hydroxyacyl-CoA dehydrogenase from Archaeoglobus fulgidus (658 aa), FASTA scores: opt: 116, E(): 0.81, (34.15% identity in 82 aa overlap); and other enzymes. And similar to other hydratases from Mycobacterium tuberculosis e.g. O53418|ECH8_MYCTU|Rv1070c|MT1100|MTV017.23c probable enoyl-CoA hydratase (257 aa), FASTA scores: opt: 111, E(): 0.83, (36.05% identity in 86 aa overlap). This homology extends across the upstream TGA stop codon into the upstream ORF MTV004.28, suggesting possible readthrough of the previous stop codon. Note that previously known as echA18'. Q6MWX6 No lipid metabolism Q6MWX6 GO:0004300 4.2.1.17 Q6MWX6 NC_000962.3 Mycobrowser_v5 CDS 3803919 3804791 . - 0 Rv3389c htdY Involved in fatty acid synthesis type II (fas-II) Probable 3-hydroxyacyl-thioester dehydratase HtdY Rv3389c, (MTV004.47c), len: 290 aa. Probable htdY, 3-hydroxyacyl-thioester dehydratase (See Gurvitz et al., 2009), shows structural similarity to six others in Mycobacterium tuberculosis (see Castell et al., 2005) especially Rv3538. Also shows similarity to members of short-chain dehydrogenases/reductases (SDR) family e.g. Q9L009|SCC30.12c putative dehydrogenase from Streptomyces coelicolor (333 aa), FASTA scores: opt: 602, E(): 2.7e-30, (40.35% identity in 305 aa overlap); Q19058|E04F6.3 hydratase-dehydrogenase-epimerase from Caenorhabditis elegans (298 aa), FASTA scores: opt: 573, E(): 1.6e-28, (41.0% identity in 266 aa overlap); Q9LBK1|PHAJ2|PA1018 (R)-specific enoyl-CoA hydratase from Pseudomonas aeruginosa (288 aa), FASTA scores: opt: 601, E(): 2.7e-30, (40.5% identity in 294 aa overlap). I6YBZ8 No intermediary metabolism and respiration 3KHP Q11198 I6YBZ8 GO:0016491,GO:0055114 1.-.-.- I6YBZ8 NC_000962.3 Mycobrowser_v5 CDS 3822262 3823863 . - 0 Rv3403c Rv3403c Unknown Hypothetical protein Rv3403c, (MTCY78.25), len: 533 aa. Hypothetical unknown protein, but some weak similarity to Q9KJP2 hypothetical 54.9 KDA protein from Myxococcus xanthus (504 aa), FASTA scores: opt: 157, E(): 0.011, (24.1% identity in 548 aa overlap). P9WKZ5 No conserved hypotheticals P9WKZ5 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 3795100 3796086 . - 0 Rv3380c Rv3380c Involved in the transposition of the insertion sequence IS6110. Probable transposase Rv3380c, (MTV004.38c), len: 328 aa. Probable transposase subunit for IS6110. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv3380c and Rv3381c, the sequence UUUUAAAG (directly upstream of Rv3380c) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 34 aa). P9WKH9 No insertion seqs and phages P0C5G8 P9WKH9 GO:0015074,GO:0006310,GO:0032196,GO:0003677 P9WKH9 NC_000962.3 Mycobrowser_v5 CDS 3792358 3793248 . - 0 Rv3378c Rv3378c Produces isotuberculosinol (nosyberkol) from halimadienyl diphosphate. Production of tuberculosinol has also been shown by some. Diterpene synthase Rv3378c, (MTV004.36c), len: 296 aa. Diterpene synthase. Note that this ORF and the downstream ORF MTV004.35c have a significantly lower GC bias than the rest of the genome. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Cofactor: Mg2+. P9WJ61 No intermediary metabolism and respiration P9WJ61 P9WJ61 NC_000962.3 Mycobrowser_v5 CDS 3799243 3799635 . - 0 Rv3384c vapC46 Unknown Possible toxin VapC46. Contains PIN domain. Rv3384c, (MTV004.42c), len: 130 aa. Possible vapC46, toxin, part of toxin-antitoxin (TA) operon with Rv3385c, contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. P95252|Rv1962c|MTCY09F9.02 (135 aa), FASTA scores: opt: 266, E(): 1.6e-10, (43.1% identity in 130 aa overlap); and Q50717|YY08_MYCTU|Rv3408|MTCY78.20c (136 aa), FASTA scores: opt: 243, E(): 4.8e-09, (35.1% identity in 131 aa overlap). O50411 No virulence, detoxification, adaptation O50411 O50411 O50411 NC_000962.3 Mycobrowser_v5 CDS 3799635 3799943 . - 0 Rv3385c vapB46 Unknown Possible antitoxin VapB46 Rv3385c, (MTV004.43c), len: 102 aa. Possible vapB46, antitoxin, part of toxin-antitoxin (TA) operon with Rv3386c, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis hypothetical proteins e.g. Q50718|Y09M_MYCTU|MTCY78.21c|Rv3407|MT3515 (99 aa), FASTA scores: opt: 155, E(): 0.001, (41.05% identity in 78 aa overlap); O07782|Rv0596c|MTCY19H5.26 (85 aa), FASTA scores: opt: 136, E(): 0.016, (39.45% identity in 71 aa overlap); P96916|Rv0626|MTCY20H10.07 (86 aa), FASTA scores: opt: 130, E(): 0.04, (51.2% identity in 41 aa overlap); etc. Also similar to prevent host death (PHD) proteins e.g. CAA66834|PHD from Escherichia coli (73 aa), FASTA scores: opt: 113, E(): 0.45, (39.4% identity in 66 aa overlap); and Q06253|PHD_BPP1 from Bacteriophage P1 (73 aa), FASTA scores: opt: 113, E(): 0.45, (39.4% identity in 66 aa overlap). P9WF13 No virulence, detoxification, adaptation O50412 P9WF13 P9WF13 NC_000962.3 Mycobrowser_v5 CDS 3800092 3800796 . + 0 Rv3386 Rv3386 Involved in the transposition of the insertion sequence IS1560. Possible transposase Rv3386, (MTV004.44), len: 234 aa. Possible transposase, showing very weak similarity to several is element transposases. Highly similar (but shorter) to P963659|MTCY10G2_13|Rv1036c from Mycobacterium tuberculosis (112 aa), FASTA scores: opt: 507, E(): 8.3e-25, (83.9% identity in 87 aa overlap). O50413 No insertion seqs and phages O50413 O50413 NC_000962.3 Mycobrowser_v5 CDS 3804865 3805575 . + 0 Rv3390 lpqD Unknown Probable conserved lipoprotein LpqD Rv3390, (MTV004.48), len: 236 aa. Probable lpqD, a conserved lipoprotein with some similarity to various bacterial proteins e.g. Q9F3Q7|SC10F4.03 putative isomerase from Streptomyces coelicolor (224 aa), FASTA scores: opt: 416, E(): 2.5e-18, (33.0% identity in 197 aa overlap); Q9ZAX0|PGM 2,3-PDG dependent phosphoglycerate mutase from Amycolatopsis methanolica (205 aa), FASTA scores: opt: 314, E(): 3.7e-12, (28.55% identity in 203 aa overlap); P73454|SLR1748 hypothetical 24.2 KDA protein from Synechocystis sp. strain PCC 6803 (214 aa), FASTA scores: opt: 201, E(): 2.8e-05, (23.8% identity in 189 aa overlap); etc. Also similar to Mycobacterium tuberculosis hypothetical proteins e.g. O53817|Rv0754|MTV041.28 PGRS-family protein (584 aa), FASTA scores: opt: 219, E(): 5.1e-06, (39.8% identity in 226 aa overlap). Contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O50416 No cell wall and cell processes O50416 O50416 O50416 NC_000962.3 Mycobrowser_v5 CDS 3805621 3807573 . + 0 Rv3391 acrA1 Function unknown; involved in cellular metabolism Possible multi-functional enzyme with acyl-CoA-reductase activity AcrA1 Rv3391, (MTV004.49), len: 650 aa. Possible acrA1, multi functional protein with fatty acyl-CoA reductase activity in C-terminal part. Indeed C-terminal part highly similar to P94129|ACR1 fatty acyl-CoA reductase from Acinetobacter calcoaceticus (295 aa), FASTA scores: opt: 767, E(): 1.4e-36, (45.4% identity in 260 aa overlap); and similar to other oxidoreductases dehydrogenases/reductases e.g. Q9Y3A1 CGI-93 protein (similarity with SDR family) from Homo sapiens (Human) (291 aa), FASTA scores: opt: 363, E(): 1.5e-13, (38.65% identity in 194 aa overlap); Q9L146|SC6D11.09 putative oxidoreductase (similarity with SDR family) from Streptomyces coelicolor (343 aa), FASTA scores: opt: 346, E(): 1.6e-12, (30.4% identity in 283 aa overlap); Q9HSR4|YUSZ1|VNG0115G oxidoreductase from Halobacterium sp. strain NRC-1 (260 aa), FASTA scores: opt: 338, E(): 3.7e-12, (33.85% identity in 248 aa overlap); etc. C-terminus also similar to Mycobacterium tuberculosis proteins Q10783|YF43_MYCTU|Rv1543|MTCY48.22c putative oxidoreductase (341 aa), FASTA scores: opt: 787, E(): 1.2e-37, (39.8% identity in 319 aa overlap); O06413|Rv0547c|MTCY25D10.26c hypothetical 31.8 KDA protein (294 aa), FASTA scores: opt: 565, E(): 4.7e-25, (36.8% identity in 242 aa overlap); O53398|Rv1050|MTV017.03 oxidoreductase (SDR family) (301 aa), FASTA scores: opt: 436, E(): 1.1e-17, (32.2% identity in 292 aa overlap). N-terminus (aa 1-320) is similar to P37693|HETM_ANASP polyketide synthase hetM from Anabaena sp. (506 aa), FASTA scores: opt: 188, E(): 1.3e-07, (27.7% identity in 361 aa overlap); so certainly a multi-domain enzyme. Seems to belong to the short-chain dehydrogenases/reductases (SDR) family. Note that this ORF corresponds to the gene ORF2|Q11197 (see Yuan et al., 1995), but longer 266 aa, due to use of a more upstream start site. O50417 No lipid metabolism O50417 O50417 GO:0055114,GO:0016491 1.2.1.- O50417 NC_000962.3 Mycobrowser_v5 CDS 3823880 3824584 . - 0 Rv3404c Rv3404c Unknown Conserved hypothetical protein Rv3404c, (MTCY78.24), len: 234 aa. Conserved hypothetical protein, some similarity to several methionyl-tRNA formyltransferases e.g. BAB51418|MLL4854 from Rhizobium loti (Mesorhizobium loti) (317 aa), FASTA scores: opt: 210, E(): 1.7e-06, (27.55% identity in 178 aa overlap); P94463|FMT_BACSU from Bacillus subtilis (317 aa), FASTA scores: opt: 199 ,E(): 8.8e-06, (28.25% identity in 177 aa overlap); O51091||FMT_BORBU|BB0064 from Borrelia burgdorferi (Lyme disease spirochete) (312 aa), FASTA scores: opt: 187, E(): 5.2e-05, (30.2% identity in 192 aa overlap); etc. P9WKZ3 No conserved hypotheticals P65073 P9WKZ3 GO:0016742,GO:0009058 P9WKZ3 NC_000962.3 Mycobrowser_v5 CDS 4036731 4038050 . - 0 Rv3595c PE_PGRS59 Function unknown PE-PGRS family protein PE_PGRS59 Rv3595c, (MTCY07H7B.27), len: 439 aa. PE_PGRS59, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), similar to many e.g. O53439|Rv1091|MTV017.44 (853 aa), FASTA scores: opt: 1644, E(): 1.2e-57, (58.75% identity in 492 aa overlap). Q6MWV6 No PE/PPE Q6MWV6 Q6MWV6 Q6MWV6 NC_000962.3 Mycobrowser_v5 CDS 3809442 3811025 . - 0 Rv3394c Rv3394c Function unknown Conserved hypothetical protein Rv3394c, (MTV004.52c), len: 527 aa. Hypothetical protein, with some similarity to various bacterial proteins e.g. BAB51085|MLR4427 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (545 aa), FASTA scores: opt: 267, E(): 2.8e-08, (26.5% identity in 509 aa overlap); BAB48362|MLR0866 DNA damage inducible protein P from Rhizobium loti (Mesorhizobium loti) (438 aa), FASTA scores: opt: 245, E(): 4.6e-07, (25.5% identity in 290 aa overlap); Q9S292|SCI11.27c hypothetical protein from Streptomyces coelicolor (322 aa), FASTA scores: opt: 202, E(): 0.00012, (28.5% identity in 323 aa overlap); etc. Also similarity with P95102|DINP|RV3056|MTCY22D7.25c hypothetical protein from Mycobacterium tuberculosis (346 aa), FASTA scores: opt: 211, E(): 3.9e-05, (26.45% identity in 306 aa overlap). Equivalent to AAK47838 from Mycobacterium tuberculosis strain CDC1551 (492 aa) but longer 35 aa. O50419 No conserved hypotheticals O50419 O50419 GO:0003887,GO:0003684,GO:0006281 O50419 NC_000962.3 Mycobrowser_v5 CDS 3833038 3833676 . - 0 Rv3414c sigD The sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released. Probable alternative RNA polymerase sigma-D factor SigD Rv3414c, (MTCY78.15), len: 212 aa. Probable sigD, alternative RNA polymerase sigma-D factor (see citations below), similar to others (notably from Streptomyces coelicolor) e.g. Q9L0I8|SCD63.01 from Streptomyces coelicolor (195 aa), FASTA scores: opt: 533, E(): 9.6e-28, (47.25% identity in 182 aa overlap); Q9FDS3|ADSA from Streptomyces griseus (258 aa), FASTA scores: opt: 223, E(): 1.8e-07, (28.95% identity in 183 aa overlap); BAB48649|MLL1224 from Rhizobium loti (Mesorhizobium loti) (187 aa), FASTA scores: opt: 202, E(): 3.2e-06, (30.4% identity in 194 aa overlap); P38133|RPOE_STRCO|SIGE|SCE94.07 from Streptomyces coelicolor (176 aa), FASTA scores: opt: 200, E(): 4.1e-06, (35.25% identity in 156 aa overlap); P37978|CNRH_ALCEU from Alcaligenes eutrophus (Ralstonia eutropha), FASTA scores: opt: 197, E(): 6.9e-06, (30.35% identity in 191 aa overlap); etc. C-terminus strongly similar to N-terminal part of Q49727|S1620B|B1620_C3_233 hypothetical 6.2 KDA protein from Mycobacterium leprae (59 aa), FASTA scores: opt: 217, E(): 1.3e-07, (90.25% identity in 41 aa overlap). Belongs to the sigma-70 factor family. P9WGG9 No information pathways P66811 P9WGG9 GO:0016987,GO:0003700,GO:0006352,GO:0006355 P9WGG9 NC_000962.3 Mycobrowser_v5 CDS 3853215 3853928 . - 0 Rv3434c Rv3434c Unknown Possible conserved transmembrane protein Rv3434c, (MTCY77.06c), len: 237 aa. Possible conserved transmembrane protein, showing some similarity with Q9CGH7|YLDB hypothetical protein from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (258 aa), FASTA scores: opt: 248, E(): 1.6e-09, (28.8% identity in 198 aa overlap); and P94983|Rv1648|MTCY06H11.13 from Mycobacterium tuberculosis (268 aa), FASTA scores: opt: 205, E(): 1.2e-06, (31.45% identity in 194 aa overlap). I6YC44 No cell wall and cell processes I6YC44 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 3815027 3816106 . - 0 Rv3398c idsA1 Involved in the biosynthesis of membrane ether-linked lipids. Catalyzes the trans-addition of the three molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate which is a precursor of the ether-linked lipids [catalytic ACTIVITY1: dimethylallyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate] [catalytic ACTIVITY2: geranyl diphosphate + isopentenyl diphosphate = diphosphate + trans,trans-farnesyl diphosphate] [catalytic ACTIVITY3: trans-trans-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate] Probable multifunctional geranylgeranyl pyrophosphate synthetase IdsA1 (GGPP synthetase) (ggppsase) (geranylgeranyl diphosphate synthase): dimethylallyltransferase (prenyltransferase) (geranyl-diphosphate synthase) + geranyltranstransferase (farnesyl-diphosphate synthase) (farnesyl-pyrophosphate synthetase) (farnesyl diphosphate synthetase) (FPP synthetase) + farnesyltranstransferase (geranylgeranyl-diphosphate synthase) Rv3398c, (MTCY78.30), len: 359 aa. Probable idsA1, geranylgeranyl pyrophosphate synthetase (GGPP synthetase) including: dimethylallyltransferase , geranyltranstransferase, and farnesyltranstransferase. Most similar to AE000797_3|O26156|Q53479 bifunctional short chain isoprenyl diphosphate synthase from Methanobacterium thermoautotrop (325 aa), FASTA scores: opt: 605, E(): 0, (37.1% identity in 329 aa overlap); homology suggests ATG at 30121 or TTG at 30145 to be the initiation codon. Contains PS00444 Polyprenyl synthetases signature 2. Belongs to the FPP/GGPP synthetases family; belongs to a family that groups together FPP synthetase, GGPP synthetase and hexaprenyl pyrophosphate synthetase. Note that previously known as idsA. P9WKH1 No lipid metabolism P0A5H8 P9WKH1 GO:0004311,GO:0004337,GO:0008299,GO:0046872,GO:0004161 2.5.1.10,2.5.1.29,2.5.1.1 P9WKH1 NC_000962.3 Mycobrowser_v5 CDS 3816129 3817175 . + 0 Rv3399 Rv3399 Possible methyltransferase Possible S-adenosylmethionine-dependent methyltransferase Rv3399, (MTCY78.29c), len: 348 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), similar to other Mycobacterium tuberculosis (strains H37Rv and CDC1551) hypothetical proteins e.g. P95074|Rv0726c|MTCY210.45c (367 aa), FASTA scores: opt: 1188, E(): 7.7e-69, (60.05% identity in 308 aa overlap); MTCY31.21c (38.0% identity in 308 aa overlap), MTV041_5, MTCY4C12_14, MTY13D12_21, MTV043_22, MTCY210_44, MTCI5_19, MTCI5_20, MTV035_9, MTCY180_22, MTCY31_23, MTY13D12_1, MTCY180_29; etc. P9WFH1 No lipid metabolism Q50726 P9WFH1 GO:0008168 2.1.1.- P9WFH1 NC_000962.3 Mycobrowser_v5 CDS 3818042 3820402 . + 0 Rv3401 Rv3401 Function unknown; probably enzyme involved in cellular metabolism. Conserved protein Rv3401, (MTCY78.27c), len: 786 aa. Conserved protein, may be an hydrolase or a transferase, equivalent to Q49736|ML0392|B1620_F1_30 hypothetical 88.1 KDA protein from Mycobacterium leprae (792 aa), FASTA scores: opt: 4820, E(): 0, (91.45% identity in 782 aa overlap). Also highly similar to Q9L2I8|SCF42.31c putative glycosyl transferase from Streptomyces coelicolor (792 aa), FASTA scores: opt: 3060, E(): 2.9e-179, (59.25% identity in 785 aa overlap); and similar to others e.g. Q9K109|NMB0390 maltose phosphorylase from Neisseria meningitidis (serogroup B) (752 aa), FASTA scores: opt: 980, E(): 3.5e-52, (29.2% identity in 774 aa overlap); Q9JSW8|MAPA|NMA2098 putative maltose phosphorylase from Neisseria meningitidis (serogroup A) (752 aa), FASTA scores: opt: 956, E(): 1e-50, (28.4% identity in 764 aa overlap); O06993|YVDK_BACSU hypothetical 88.3 KDA protein (belongs to family 65 of glycosyl hydrolases) from Bacillus subtilis (757 aa), FASTA scores: opt: 926, E(): 6.9e-49, (28.5% identity in 754 aa overlap); Q9CF04|MAPA maltosephosphorylase from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (751 aa), FASTA scores: opt: 907, E(): 1e-47, (26.95% identity in 753 aa overlap); P77154|YCJT_ECOLI|B1316 hypothetical 84.9 KDA protein (belongs to family 65 of glycosyl hydrolases) from Escherichia coli strain K12 (755 aa), FASTA scores: opt: 392, E(): 2.9e-16, (27.5% identity in 774 aa overlap); etc. Also similar to Mycobacterium tuberculosis hypothetical protein Q10850|YK06_MYCTU|Rv2006|MT2062|MTCY39.11c (1327 aa), (27.2% identity in 802 aa overlap); note that Rv3400 and Rv3401 are similar to beginning and end of Q10850|YK06_MYCTU|Rv2006|MT2062|MTCY39.11c with approx. 270 aa missing from the middle. P9WN13 No intermediary metabolism and respiration Q50724 P9WN13 GO:0005975,GO:0016798,GO:0030246 3.2.1.- P9WN13 NC_000962.3 Mycobrowser_v5 CDS 3824702 3825268 . - 0 Rv3405c Rv3405c May be involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv3405c, (MTCY78.23), len: 188 aa. Possible transcriptional regulator, showing weak similarity to other bacterial regulatory proteins e.g. Q9KE70|BH0987 from Bacillus halodurans (203 aa), FASTA scores: opt: 168, E(): 0.0016, (34.8% identity in 92 aa overlap); Q9A5F7|CC2493 Caulobacter crescentus (204 aa), FASTA scores: opt: 160, E(): 0.0051, (32.6% identity in 89 aa overlap); Q9RDR0|SC4A7.02 from Streptomyces coelicolor (227 aa), FASTA scores: opt: 159, E(): 0.0064, (37.0% identity in 189 aa overlap); etc. Also some similarity to hypothetical Mycobacterium tuberculosis regulatory proteins e.g. O05858|Rv3208|MTCY07D11.18c, MTCI125_6, MTCY7D11_18, MTCY10G2_30; etc. Contains potential helix-turn-helix motif from aa 39-60 (+2.97 SD). P9WMC3 No regulatory proteins P67442 P9WMC3 GO:0006350,GO:0003700,GO:0006355 P9WMC3 NC_000962.3 Mycobrowser_v5 CDS 3825330 3826217 . + 0 Rv3406 Rv3406 Function unknown; involved in cellular metabolism. Probable dioxygenase Rv3406, (MTCY78.22c), len: 295 aa. Probable dioxygenase, highly similar to Q9WWU|ATSK putative alpha-ketoglutarate dependent dioxygenase from Pseudomonas putida (301 aa), FASTA scores: opt: 994, E(): 3.9e-57, (53.7% identity in 283 aa overlap); Q9I6U1|PA0193 hypothetical protein from Pseudomonas aeruginosa (300 aa), FASTA scores: opt: 1024, E(): 4.4e-59, (53.65% identity in 287 aa overlap); Q9HX81|TAUD|PA3935 taurine dioxygenase from Pseudomonas aeruginosa (277 aa), FASTA scores: opt: 599, E(): 1.4e-31, (39.35% identity in 277 aa overlap); and similar to other dioxygenases e.g. AAG54718|TAUD (alias BAB33845|ECS0422) taurine dioxygenase 2-oxoglutarate-dependent from Escherichia coli strain O157:H7 (283 aa), FASTA scores: opt: 595, E(): 2.5e-31, (38.1% identity in 281 aa overlap); etc. Belongs to the TfdA family of dioxygenases. P9WKZ1 No intermediary metabolism and respiration P65075 P9WKZ1 GO:0016702,GO:0055114 1.-.-.- P9WKZ1 NC_000962.3 Mycobrowser_v5 CDS 3826252 3826551 . + 0 Rv3407 vapB47 Unknown Possible antitoxin VapB47 Rv3407, (MTCY78.21c), len: 99 aa. Possible vapB47, antitoxin, part of toxin-antitoxin (TA) operon with Rv3408, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. AAK46285|MT2013 (90 aa), FASTA scores: opt: 160, E(): 0.00021, (37.1% identity in 89 aa overlap); O50412|Rv3385c|MTV004.43c (102 aa), FASTA scores: opt: 155, E(): 0.00051, (41.05% identity in 78 aa overlap), MTCY19H5.26, MTCY20H10.07, MTI376.09c, MTCY427.21, etc. P9WF23 No virulence, detoxification, adaptation P65077 P9WF23 P9WF23 NC_000962.3 Mycobrowser_v5 CDS 3826991 3828727 . - 0 Rv3409c choD Involved in cholesterol metabolism [catalytic activity: cholesterol + O(2) = cholest-4-en-3-one + H(2)O(2)]. Cholesterol oxidase ChoD (cholesterol-O2 oxidoreductase) Rv3409c, (MTCY78.19), len: 578 aa. ChoD, cholesterol oxidase, equivalent to Q9CCV1|CHOD|ML0389 (alias Q59530|CHOD|B1620_C3_240) putative cholesterol oxidase from Mycobacterium leprae (569 aa), FASTA scores: opt: 3510, E(): 3.8e-198, (88.6% identity in 569 aa overlap). Belongs to the GMC oxidoreductases family. Cofactor: FAD flavoprotein. Contains PS00017 ATP/GTP-binding site motif A. P9WMV9 No lipid metabolism Q57307 P9WMV9 GO:0055114,GO:0016995 1.1.3.6 P9WMV9 NC_000962.3 Mycobrowser_v5 CDS 3843885 3844640 . - 0 Rv3427c Rv3427c Involved in the transposition of the insertion sequence IS1532. Possible transposase Rv3427c, (MTCY78.02), len: 251 aa. Possible transposase, similar to other e.g. Q9APG8|ORF2 putative transposase subunit 2 from Pseudomonas putida (251 aa), FASTA scores: opt: 479, E(): 1.8e-21, (34.85% identity in 238 aa overlap). Contains PS00017 ATP/GTP-binding site motif A. Q50701 No insertion seqs and phages Q50701 Q50701 GO:0017111,GO:0005524 Q50701 NC_000962.3 Mycobrowser_v5 CDS 3829930 3831519 . - 0 Rv3411c guaB2 Catalyses the first reaction unique to GMP biosynthesis [catalytic activity: inosine 5'-phosphate + NAD(+) + H(2)O = xanthosine 5'-phosphate + NADH]. Probable inosine-5'-monophosphate dehydrogenase GuaB2 (imp dehydrogenase) (inosinic acid dehydrogenase) (inosinate dehydrogenase) (imp oxidoreductase) (inosine-5'-monophosphate oxidoreductase) (IMPDH) (IMPD) Rv3411c, (MTCY78.17), len: 529 aa. Probable guaB2, inosine-5'-monophosphate (imp) dehydrogenase, equivalent to Q49729|IMDH_MYCLE|GUAB|ML0387|B1620_C3_238 inosine-5'-monophosphate dehydrogenase from Mycobacterium leprae (529 aa), FASTA scores: opt: 3154, E(): 4.4e-165, (92.45% identity in 529 aa overlap). Highly similar to other inosine-5'-monophosphate dehydrogenases e.g. Q9RHZ0|GUAB from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (506 aa), FASTA scores: opt: 2284, E(): 1.5e-117, (67.9% identity in 505 aa overlap); Q9L0I7|SCD63.02 from Streptomyces coelicolor (501 aa), FASTA scores: opt: 2178, E(): 9e-112, (67.2% identity in 491 aa overlap); O67820|IMDH_AQUAE|GUAB|AQ_2023 from Aquifex aeolicus (490 aa), FASTA scores: opt: 1820, E(): 3.2e-92, (58.1% identity in 487 aa overlap); etc. Also similar to Q50716|YY10_MYCTU|Rv3410c|MT3518|MTCY78.18 hypothetical 38.9 KDA protein from Mycobacterium tuberculosis (38.6% identity in 158 aa overlap). Contains PS00487 imp dehydrogenase / GMP reductase signature. Similar to other eukaryotic and prokaryotic IMPDH and to GMP reductase. P9WKI7 No intermediary metabolism and respiration P65167 P9WKI7 GO:0003938,GO:0046872,GO:0055114,GO:0006177 1.1.1.205 P9WKI7 NC_000962.3 Mycobrowser_v5 CDS 3831726 3832136 . + 0 Rv3412 Rv3412 Function unknown Conserved hypothetical protein Rv3412, (MTCY78.16c), len: 136 aa. Hypothetical protein, strongly similar to Q49742|YY12_MYCLE|ML0386|B1620_F3_131 hypothetical 15.3 KDA protein from Mycobacterium leprae (137 aa), FASTA scores: opt: 933, E(): 6.3e-52, (93.4% identity in 136 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WKY9 No conserved hypotheticals P9WKY9 NC_000962.3 Mycobrowser_v5 CDS 3847165 3847701 . + 0 Rv3429 PPE59 Function unknown PPE family protein PPE59 Rv3429, (MTCY77.01), len: 178 aa. PPE59, Member of the M. tuberculosis PPE family, similar to many e.g. the upstream Q50703|YY25_MYCTU|Rv3425|MTCY78.04c (176 aa), FASTA scores: opt: 781, E(): 1.9e-44, (69.9% identity in 176 aa overlap); and Q50702|YY26_MYCTU|Rv3426|MTCY78.03c (232 aa), FASTA scores: opt: 555, E(): 1.7e-29, (72.0% identity in 125 aa overlap) (but diverges at 3' end); etc. P9WHY1 No PE/PPE O06246 P9WHY1 P9WHY1 NC_000962.3 Mycobrowser_v5 CDS 3880907 3881764 . + 0 Rv3463 Rv3463 Function unknown Conserved protein Rv3463, (MTCY13E12.16), len: 285 aa. Conserved protein, similar to Q9RDA2|SCE20.23 hypothetical 31.4 KDA protein from Streptomyces coelicolor (290 aa), FASTA scores: opt: 770, E(): 2.2e-41, (48.6% identity in 247 aa overlap); and Q9X7Y1|SC6A5.35 putative oxidoreductase from Streptomyces coelicolor (341 aa), (see blastp results), FASTA scores: opt: 119, E(): 2.9, (24.1% identity in 274 aa overlap). I6X7D4 No conserved hypotheticals O06328 I6X7D4 GO:0016491,GO:0055114 I6X7D4 NC_000962.3 Mycobrowser_v5 CDS 3851792 3853213 . - 0 Rv3433c Rv3433c Function unknown Conserved protein Rv3433c, (MTCY77.05), len: 473 aa. Conserved protein, member of YKL151c/yjeF family, equivalent to P37391|YY33_MYCLE|ML0373|U229G|B229_C2_201 hypothetical 47.2 KDA protein from Mycobacterium leprae (473 aa), FASTA scores: opt: 2650, E(): 5e-136, (84.55% identity in 473 aa overlap). Also similar to other hypothetical bacterial proteins e.g. Q9X3W3 from Zymomonas mobilis (484 aa), FASTA scores: opt: 700, E(): 1.2e-30, (33.7% identity in 484 aa overlap); O86783|SC6G4.20c from Streptomyces coelicolor (485 aa), FASTA scores: opt: 563, E(): 3.2e-23, (48.45% identity in 489 aa overlap); Q9LC81 from Arthrobacter sp. Q36 (313 aa), FASTA scores: opt: 553, E(): 7.9e-23, (44.2% identity in 303 aa overlap); etc. Contains Pfam match to entry PF01256 hypothetical UPFOO31 family signature and PF03853 YjeF-related protein N-terminus. Belongs to the UPF0031 family. P9WF11 No conserved hypotheticals O06250 P9WF11 P9WF11 NC_000962.3 Mycobrowser_v5 CDS 3836986 3837288 . - 0 Rv3418c groES Binds to CPN60 in the presence of mg-ATP and suppresses the ATPase activity of the latter. 10 kDa chaperonin GroES (protein CPN10) (protein GroES) (BCG-a heat shock protein) (10 kDa antigen) Rv3418c, (MTCY78.11), len: 100 aa. GroES (alternate gene names: cpn10, mpt57), 10 kDa chaperonin (protein cpn10) (see citations below), equivalent to P24301|CH10_MYCLE|MOPB|GROES|CHPA|ML0380|B1620_C3_227|B229_C3_247 from Mycobacterium leprae (99 aa), FASTA scores: opt: 568, E(): 2.1e-31, (89.9% identity in 99 aa overlap). And also strongly identical to others e.g. O86017|CH10_MYCAV|MOPB|GROES from Mycobacterium avium and Mycobacterium paratuberculosis (99 aa), FASTA scores: opt: 611, E(): 2.9e-34, (96.95% identity in 99 aa overlap); P15020|CH10_MYCBO|MOPB|GROES from Mycobacterium bovis (99 aa), FASTA scores: opt: 596, E(): 2.9e-33, (98.95% identity in 94 aa overlap); P40172|CH10_STRCO|GROES|SC6G4.39 from Streptomyces coelicolor and Streptomyces lividans (102 aa), FASTA scores: opt: 480, E(): 1.6e-25, (76.75% identity in 99 aa overlap); etc. Also identical to MSG10KAG_1, MT10KAG_1, MTBCGA_1. Contains PS00681 Chaperonins cpn10 signature. Belongs to the GROES chaperonin family. P9WPE5 No virulence, detoxification, adaptation 1HX5,1P3H,1P83 P09621 P9WPE5 GO:0005737,GO:0006457,GO:0006950,GO:0005524 P9WPE5 NC_000962.3 Mycobrowser_v5 CDS 3840194 3841420 . - 0 Rv3423c alr Provides the D-alanine required for cell wall biosynthesis. Transforms L-alanine to D-alanine [catalytic activity: L-alanine = D-alanine] Alanine racemase Alr Rv3423c, (MTCY78.06), len: 408 aa. Alr, alanine racemase, equivalent to P38056|ALR_MYCLE|ML0375|B229_C3_243 alanine racemase from Mycobacterium leprae (388 aa), FASTA scores: opt: 2160, E(): 2.3e-124, (84.35% identity in 384 aa overlap). Also highly similar to other alanine racemases e.g. Q9L888|ALR_MYCAV from Mycobacterium avium (391 aa), FASTA scores: opt: 2103, E(): 6.8e-121, (83.6% identity in 384 aa overlap); P94967|ALR_MYCSM from Mycobacterium smegmatis (389 aa), FASTA scores: opt: 1721, E(): 1.3e-97, (67.25% identity in 385 aa overlap); O86786|ALR_STRCO|SC6G4.23 from Streptomyces coelicolor (391 aa), FASTA scores: opt: 1041, E(): 3.7e-56, (47.65% identity in 380 aa overlap); etc. Contains Pfam entry PF00842 Alanine racemase. Belongs to the alanine racemase family. P9WQA9 No intermediary metabolism and respiration 1XFC P0A4X2 P9WQA9 GO:0008784,GO:0007047,GO:0009252,GO:0030170,GO:0008360,GO:0006522 5.1.1.1 P9WQA9 NC_000962.3 Mycobrowser_v5 CDS 3838586 3839062 . - 0 Rv3420c rimI Acetylates the N-terminal alanine of ribosomal protein S18 [catalytic activity: acetyl-CoA + ribosomal-protein L-alanine = CoA + ribosomal-protein N-acetyl-L-alanine]. Ribosomal-protein-alanine acetyltransferase RimI (acetylating enzyme for N-terminal of ribosomal protein S18) Rv3420c, (MTCY78.09), len: 158 aa. Probable rimI, ribosomal-protein-alanine acetyltransferase, contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Equivalent to C-terminal part of Q49857|YY21_MYCLE|ML0378|B229_C1_170 hypothetical 38.0 KDA protein from Mycobacterium leprae (359 aa), FASTA scores: opt: 772, E(): 2.7e-44, (72.1% identity in 154 aa overlap). Similar notably to ribosomal-protein-alanine acetyltransferases e.g. Q9AC11|CC0058 from Caulobacter crescentus (150 aa), FASTA scores: opt: 223, E(): 4.9e-08, (37.5% identity in 136 aa overlap); Q9KFD4|BH0547 from Bacillus halodurans (151 aa), FASTA scores: opt: 222, E(): 5.8e-08, (35.2% identity in 142 aa overlap); Q9PG61|XF0441 from Xylella fastidiosa (156 aa), FASTA scores: opt: 207, E(): 5.9e-07, (32.2% identity in 149 aa overlap); Q9HVB7|RIMI|PA4678 from Pseudomonas aeruginosa (150 aa), FASTA scores: opt: 203, E(): 1.1e-06, (32.45% identity in 151 aa overlap); P09453|RIMI_ECOLI|B4373 from Escherichia coli strain K12 (148 aa), FASTA scores: opt: 196, E(): 3.1e-06, (33.55% identity in 149 aa overlap); etc. Belongs to the acetyltransferase family, RIMI subfamily. I6YG32 No information pathways Q50708 I6YG32 GO:0008999,GO:0005840,GO:0006474 2.3.1.128 I6YG32 NC_000962.3 Mycobrowser_v5 CDS 3839059 3839694 . - 0 Rv3421c Rv3421c Function unknown Conserved hypothetical protein Rv3421c, (MTCY78.08), len: 211 aa. Conserved hypothetical protein, equivalent to Q49857|YY21_MYCLE|ML0378|B229_C1_170 hypothetical 38.0 KDA protein from Mycobacterium leprae (359 aa), FASTA scores: opt: 1000, E(): 1.8e-50, (75.6% identity in 205 aa overlap). Also similar to other hypothetical bacterial proteins e.g. O86791|SC6G4.28 from Streptomyces coelicolor (217 aa), FASTA scores: opt: 453, E(): 3.3e-19, (48.1% identity in 212 aa overlap); Q9AC10|CC0059 (glycoprotease family protein) from Caulobacter crescentus (211 aa), FASTA scores: opt: 248, E(): 2e-07, (34.3% identity in 210 aa overlap); Q9KQK9|VC1989 from Vibrio cholerae (237 aa), FASTA scores: opt: 238, E(): 8.2e-07, (28.85% identity in 208 aa overlap); BAB51966|Mlr5530 from Rhizobium loti (Mesorhizobium loti) (225 aa), FASTA scores: opt: 237, E(): 9e-07, (35.0% identity in 220 aa overlap); etc. Some similarity to upstream Q50709|GCP_MYCTU|Rv3419c|MT3528|MTCY78.10 from Mycobacterium tuberculosis (344 aa), (33.9% identity in 127 aa overlap). P9WKY7 No conserved hypotheticals P65083 P9WKY7 GO:0006508,GO:0004222 P9WKY7 NC_000962.3 Mycobrowser_v5 CDS 3844738 3845970 . - 0 Rv3428c Rv3428c Involved in the transposition of the insertion sequence IS1532. Possible transposase Rv3428c, (MTCY78.01, len: 410 aa. Possible transposase insertion sequence, similar to others e.g. Q9APG9|ORF1 from Pseudomonas putida (509 aa), FASTA scores: opt: 578, E(): 1.1e-29, (32.45% identity in 376 aa overlap); P55379|Y4BL_RHISN from Rhizobium sp. strain NGR234 (516 aa), FASTA scores: opt: 665, E(): 2.7e-35, (35.3% identity in 391 aa overlap); etc. Q50700 No insertion seqs and phages Q50700 Q50700 GO:0015074,GO:0003677 Q50700 NC_000962.3 Mycobrowser_v5 CDS 3847642 3848805 . - 0 Rv3430c Rv3430c Involved in the transposition of the insertion sequence IS1540. Possible transposase Rv3430c, (MTCY77.02c), len: 387 aa. Possible IS1540 transposase, similar to several e.g. Q49592 transposase from Mycobacterium intracellulare (340 aa), FASTA scores: opt: 1377, E(): 1.6e-81, (64.2% identity in 338 aa overlap); similarity is lost at C-terminus due to possible frameshift after aa 297. I6YC39 No insertion seqs and phages O06247 I6YC39 GO:0015074,GO:0003677 I6YC39 NC_000962.3 Mycobrowser_v5 CDS 3905772 3906221 . + 0 Rv3486 Rv3486 Function unknown Conserved protein Rv3486, (MTCY13E12.39), len: 149 aa. Conserved protein, similar to Q9RC47|YFID|BH3304 hypothetical protein from Bacillus halodurans (129 aa), FASTA scores: opt: 186, E(): 2.1e-05, (40.0% identity in 95 aa overlap); and Q9KKT1|VCA1019 hypothetical protein from Vibrio cholerae (148 aa), FASTA scores: opt: 128, E(): 0.15, (35.25% identity in 139 aa overlap). Some similarity to other proteins e.g. P54720|YFID_BACSU hypothetical protein from Bacillus subtilis (134 aa), FASTA scores: opt: 165, E(): 0.00052, (31.75% identity in 126 aa overlap). Equivalent to AAK47949 from Mycobacterium tuberculosis strain CDC1551 (163 aa) but shorter 14 aa. O06349 No conserved hypotheticals O06349 O06349 NC_000962.3 Mycobrowser_v5 CDS 3853939 3854793 . - 0 Rv3435c Rv3435c Unknown Probable conserved transmembrane protein Rv3435c, (MTCY77.07c), len: 284 aa. Probable conserved transmembrane protein, showing some similarity with P95061|Rv0713|MTCY210.32 hypothetical 33.9 KDA protein from Mycobacterium tuberculosis (313 aa), FASTA scores: opt: 557, E(): 1.3e-26, (35.8% identity in 282 aa overlap); and O32991|MLCB2492.12 from Mycobacterium leprae (95 aa), FASTA scores: opt: 150, E(): 0.022, (35.3% identity in 85 aa overlap). Equivalent to AAK47881 from Mycobacterium tuberculosis strain CDC1551 (312 aa) but shorter 28 aa. O06252 No cell wall and cell processes O06252 GO:0016021 O06252 NC_000962.3 Mycobrowser_v5 CDS 3857397 3858239 . + 0 Rv3438 Rv3438 Function unknown Conserved protein Rv3438, (MTCY77.10), len: 280 aa. Conserved protein, equivalent to Q9CCV6|ML0370 hypothetical protein from Mycobacterium leprae (289 aa), FASTA scores: opt: 1491, E(): 9.2e-81, (79.85% identity in 283 aa overlap); and highly similar (but shorter 41 aa) to Q49872|B229_F1_20 hypothetical 34.0 KDA protein from Mycobacterium leprae (324 aa), FASTA scores: opt: 1491, E(): 1e-80, (79.85% identity in 283 aa overlap). Shows some similarity to Q9KIU3|LIPA lipase from plasmid pAH114 uncultured bacterium (281 aa), FASTA scores: opt: 168, E(): 0.0081, (29.3% identity in 140 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). I6X7B3 No conserved hypotheticals I6X7B3 I6X7B3 NC_000962.3 Mycobrowser_v5 CDS 3858259 3859662 . - 0 Rv3439c Rv3439c Function unknown Conserved hypothetical alanine and proline rich protein Rv3439c, (MTCY77.11c), len: 467 aa. Conserved hypothetical ala-, pro-rich protein, similar in part to N-terminal part of Q49853|B229_C1_154 hypothetical 11.2 KDA protein from Mycobacterium leprae (103 aa), FASTA scores: opt: 265, E(): 0.0013, (51.1% identity in 90 aa overlap). I6YC49 No conserved hypotheticals I6YC49 I6YC49 NC_000962.3 Mycobrowser_v5 CDS 3859665 3859976 . - 0 Rv3440c Rv3440c Unknown Hypothetical protein Rv3440c, (MTCY77.12c), len: 103 aa. Hypothetical unknown protein. O06257 No conserved hypotheticals O06257 NC_000962.3 Mycobrowser_v5 CDS 3970705 3972453 . - 0 Rv3533c PPE62 Function unknown PPE family protein PPE62 Rv3533c, (MTCY03C7.23), len: 582 aa. PPE62, Member of the Mycobacterium tuberculosis PPE protein family, similar to many, e.g. O53309|Rv3159c|MTV014.03c (590 aa) FASTA scores: opt: 2289, E(): 2.3e-95, (63.5% identity in 600 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). P9WHX7 No PE/PPE Q7D5C5 P9WHX7 P9WHX7 NC_000962.3 Mycobrowser_v5 CDS 3861495 3861950 . - 0 Rv3442c rpsI Involved in translation mechanism. This protein is one of the assembly proteins of the 50S ribosomal subunit. 30S ribosomal protein S9 RpsI Rv3442c, (MTCY77.14c), len: 151 aa. rpsI, 30S ribosomal protein S9, equivalent to P40828|RS9_MYCLE|ML0365|B229_C2_191 30S ribosomal protein S9 (153 aa), FASTA scores: opt: 800, E(): 2.1e-42, (83.85% identity in 155 aa overlap). Also highly similar to others e.g. Q53875|RS9_STRCO|SC6G4.13 from Streptomyces coelicolor (170 aa), FASTA scores: opt: 533, E(): 5.7e-26, (60.75% identity in 135 aa overlap); Q9KGD4|RPSI|BH0169 (BS10) from Bacillus halodurans (130 aa), FASTA scores: opt: 469, E(): 3.8e-22, (58.65% identity in 121 aa overlap); Q9CDG7|RPSI from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (130 aa), FASTA scores: opt: 451, E(): 4.9e-21, (58.65% identity in 121 aa overlap); P07842|RS9_BACST|RPSI from Bacillus stearothermophilus (129 aa), FASTA scores: opt: 448, E(): 7.4e-21, (54.55% identity in 121 aa overlap); etc. Contains PS00360 Ribosomal protein S9 signature. Belongs to the S9P family of ribosomal proteins. P9WH25 No information pathways P66639 P9WH25 GO:0003735,GO:0006412,GO:0005840 P9WH25 NC_000962.3 Mycobrowser_v5 CDS 3862624 3862926 . - 0 Rv3444c esxT Function unknown Putative ESAT-6 like protein EsxT Rv3444c, (MTCY77.16c), len: 100 aa. EsxT, ESAT-6 like protein (see citation below), equivalent to Q9CCV7|ML0363 possible secreted protein from Mycobacterium leprae (104 aa), FASTA scores: opt: 362, E(): 1.1e-18, (71.25% identity in 73 aa overlap). C-terminal part highly similar to Q49852|B229_C1_150 hypothetical 5.3 KDA protein from Mycobacterium leprae (49 aa), FASTA scores: opt: 227, E(): 1.4e-09, (68.9% identity in 45 aa overlap). Seems to belong to the ESAT6 family. I6YC53 No cell wall and cell processes I6YC53 NC_000962.3 Mycobrowser_v5 CDS 3864528 3868238 . - 0 Rv3447c eccC4 Unknown, but could hydrolyse ATP/GTP. ESX conserved component EccC4. ESX-4 type VII secretion system protein. Probable membrane protein. Rv3447c, (MTCY77.19c), len: 1236 aa. EccC4, esx conserved component, ESX-4 type VII secretion system protein, probable membrane protein, similar to various bacterial proteins e.g. O86653|SC3C3.20c ATP/GTP binding protein from Streptomyces coelicolor (1321 aa), FASTA scores: opt: 1186, E(): 1.9e-60, (42.9% identity in 1312 aa overlap); Q9L0T6|SCD35.15c from Streptomyces coelicolor (1525 aa), FASTA scores: opt: 932, E(): 9.2e-46, (27.2% identity in 1374 aa overlap); Q9CD30|ML2535 hypothetical protein from Mycobacterium leprae (1329 aa), FASTA scores: opt: 910, E(): 1.5e-44, (34.4% identity in 1319 aa overlap); Q9KE81|BH0975 hypothetical protein from Bacillus halodurans (1489 aa), FASTA scores: opt: 805, E(): 1.9e-38, (25.85% identity in 1292 aa overlap); etc. The C-terminal region is similar to Q9CDD7|ML0052 (alias O33086|MLCB628.15c) hypothetical protein from Mycobacterium leprae (597 aa), FASTA scores: opt: 850, E(): 2.3e-41, (35.2% identity in 588 aa overlap); and O6973|Rv3871|MTV027.06 hypothetical protein from Mycobacterium tuberculosis (591 aa), FASTA scores: opt: 845, E(): 4.3e-41, (35.3% identity in 586 aa overlap). N-terminal part shows similarity with hypothetical proteins from Mycobacterium tuberculosis e.g. O69735|Rv3870|MTV027.05 (747 aa), FASTA scores: opt: 761, E(): 3.6e-36, (38.2% identity in 746 aa overlap). Equivalent to AAK47893 from Mycobacterium tuberculosis strain CDC1551 (1200 aa) but longer 36 aa. Contains three of PS00017 ATP/GTP-binding site motif A (P-loop). P9WNA7 No cell wall and cell processes O06264 P9WNA7 GO:0003677,GO:0007049,GO:0051301,GO:0007059,GO:0016021,GO:0017111,GO:0005886,GO:0005524 P9WNA7 NC_000962.3 Mycobrowser_v5 CDS 3894426 3895607 . + 0 Rv3478 PPE60 Function unknown PE family protein PPE60 Rv3478, (MTCY13E12.31), len: 393 aa. PPE60 (alternate gene name: mtb39c). Member of the M. tuberculosis PPE family, highly similar to others e.g. Q11031|YD61_MYCTU|Rv1361c|MT1406|MTCY02B10.25c (396 aa), FASTA scores: opt: 2165, E(): 1.1e-109, (85.35% identity in 396 aa overlap); MTCI364.08; MTCY10G2.10; MTCY03A2.22c; MTCY274.23c; MTCY164.34c; MTCY98.0029c; etc. Note that expression of Rv3478 was demonstrated in lysates by immunodetection (see Dillon et al., 1999). Q6MWX1 No PE/PPE Q6MWX1 Q6MWX1 Q6MWX1 NC_000962.3 Mycobrowser_v5 CDS 3868352 3869755 . + 0 Rv3448 eccD4 Unknown. Possibly involved in transport across the membrane. ESX conserved component EccD4. ESX-4 type VII secretion system protein. Probable integral membrane protein. Rv3448, (MTCY77.20), len: 467 aa. EccD4, esx conserved component, ESX-4 type VII secretion system protein, probable integral membrane protein, showing some similarity with Q9CD35|ML2529 from Mycobacterium leprae (485 aa), FASTA scores: opt: 371, E(): 3.6e-14, (27.25% identity in 481 aa overlap); and two proteins from Mycobacterium tuberculosis O86362|Rv0290|MTV035.18 (472 aa), FASTA scores: opt: 429, E(): 1.6e-17, (28.6% identity in 479 aa overlap); and O05457|Rv3887c|MTCY15F10.25 (509 aa), FASTA scores: opt: 203, E(): 0.00019, (25.6% identity in 492 aa overlap). Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature. P9WNQ1 No cell wall and cell processes O33354 P9WNQ1 GO:0005886,GO:0016021 P9WNQ1 NC_000962.3 Mycobrowser_v5 CDS 3869752 3871119 . + 0 Rv3449 mycP4 Thought to have proteolytic activity. Probable membrane-anchored mycosin MycP4 (serine protease) (subtilisin-like protease) (subtilase-like) (mycosin-4) Rv3449, (MTCY13E12.02), len: 455 aa. Probable mycP4, membrane-anchored serine protease (mycosin) (see citation below), similar to hypothetical unknowns or proteases from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. AAK48366|MT3998 subtilase family protein from Mycobacterium tuberculosis strain CDC1551 (411 aa), FASTA scores: opt: 747, E(): 3.5e-33, (45.65% identity in 416 aa overlap); O05461|Rv3883c|MTCY15F10.29 membrane-anchored mycosin MYCP1 (446 aa), FASTA scores: opt: 747, E(): 3.8e-33, (45.45% identity in 451 aa overlap); O53695|Rv0291|MTV035.19 probable membrane-anchored mycosin MYCP2 (461 aa), FASTA scores: opt: 660, E(): 1.9e-28, (44.0% identity in 457 aa overlap); etc. And similar to hypothetical proteases from Mycobacterium leprae e.g. O33076|MLCB628.04|ML0041 hypothetical 45.7 KDA protein (probable secreted protease) (446 aa), FASTA scores: opt: 683, E(): 1.1e-29, (43.8% identity in 450 aa overlap); Q9CD36|ML2528 putative protease (475 aa), FASTA scores: opt: 608, E(): 1.3e-25, (43.0% identity in 451 aa overlap); Q9CBV3|ML1538 possible protease (567 aa), FASTA scores: opt: 389, E(): 9.7e-14, (33.8% identity in 562 aa overlap); etc. Also some similarity to other proteases from several organisms e.g. O31788|APRX alkaline serine protease from Bacillus subtilis (442 aa), FASTA scores: opt: 296, E(): 8.3e-09, (29.4% identity in 313 aa overlap); O86650|SC3C3.17c putative secreted serine protease from Streptomyces coelicolor (450 aa), FASTA scores: opt: 279, E(): 7e-08, (33.55% identity in 343 aa overlap); Q9KBJ7|APRX|BH193 intracellular alkaline serine protease from Bacillus halodurans (444 aa), FASTA scores: opt: 257, E(): 1.1e-06, (28.65% identity in 335 aa overlap); O86642|SC3C3.08 serine protease from Streptomyces coelicolor (413 aa), FASTA scores: opt: 243, E(): 5.7e-06, (38.25% identity in 387 aa overlap); etc. Has putative signal peptide at N-terminus and hydrophobic stretch at C-terminus. Contains three signatures typical of subtilase family: aspartic acid active site (PS00136), histidine active site (PS00137), and serine active site (PS00138). Belongs to peptidase family S8 (also known as the subtilase family), pyrolysin subfamily. I6YC58 No intermediary metabolism and respiration O06316 I6YC58 GO:0004252,GO:0006508 3.4.21.- I6YC58 NC_000962.3 Mycobrowser_v5 CDS 3965884 3967038 . - 0 Rv3529c Rv3529c Function unknown Conserved hypothetical protein Rv3529c, (MTCY03C7.27), len: 384 aa. Conserved hypothetical protein, showing some similarity to Q50695|YM67_MYCTU|Rv2267c|MT2329|MTCY339.43 hypothetical 46.1 KDA protein from Mycobacterium tuberculosis (388 aa) FASTA scores: opt: 261, E(): 1.6e-09, (27.25% identity in 253 aa overlap). I6YCC4 No conserved hypotheticals 2ZQ5 P71872 I6YCC4 GO:0008146 I6YCC4 NC_000962.3 Mycobrowser_v5 CDS 4040981 4041319 . - 0 Rv3597c lsr2 Dominant T-cell antigen and possibly stimulates lymphoproliferation. Has DNA-bridging activity. Iron-regulated H-NS-like protein Lsr2 Rv3597c, (MTCY07H7B.25), len: 112 aa. Lsr2, H-NS-like protein, identical to P24094|LSR2_MYCLE|ML0234 LSR2 protein precursor (15 KDA antigen) (A15) from Mycobacterium leprae (112 aa), FASTA scores: opt: 698, E(): 6.7e-37, (92.85% identity in 112 aa overlap). Also highly similar to others e.g. Q9X8N1|SCE94.26c from Streptomyces coelicolor (111 aa), FASTA scores: opt: 379, E(): 4.4e-17, (58.05% identity in 112 aa overlap); Q9ETI2|LSR2 from Corynebacterium equii (Rhodococcus equi) (119 aa), FASTA scores: opt: 328, E(): 6.9e-14, (47.5% identity in 120 aa overlap); and Q9RKK8|SCD25.12c from Streptomyces coelicolor (105 aa), FASTA scores: opt: 293, E(): 9.4e-12, (47.75% identity in 111 aa overlap). P9WIP7 No information pathways 2KNG P9WIP7 P9WIP7 NC_000962.3 Mycobrowser_v5 CDS 3874822 3876090 . + 0 Rv3454 Rv3454 Unknown Probable conserved integral membrane protein Rv3454, (MTCY13E12.07), len: 422 aa. Probable conserved integral membrane protein, showing some similarity to various proteins (generally transporters) e.g. Q9I5C8|PA0811 probable MFS transporter from Pseudomonas aeruginosa (415 aa), FASTA scores: opt: 145, E(): 0.13, (28.2% identity in 188 aa overlap); Q01266|YHYC_PSESN hypothetical protein in HYUC 3'region (ORF 5) (fragment) from Pseudomonas sp. strain NS671 (245 aa), FASTA scores: opt: 130, E(): 0.75, (24.65% identity in 134 aa overlap); Q9I242|PA2073 probable transporter (membrane subunit) from Pseudomonas aeruginosa (476 aa), FASTA scores: opt: 125, E(): 2.5, (24.6% identity in 252 aa overlap); etc. Equivalent to AAK47900 from Mycobacterium tuberculosis strain CDC1551 (562 aa) but shorter 140 aa. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O06321 No cell wall and cell processes O06321 NC_000962.3 Mycobrowser_v5 CDS 3908236 3909738 . + 0 Rv3490 otsA Involved in osmoregulatory trehalose biosynthesis. Mycobacteria can produce trehalose from glucose 6-phosphate and UDP-glucose (the OtsA-OtsB pathway) from glycogen-like alpha(1-->4)-linked glucose polymers (the TreY-TreZ pathway) and from maltose (the TreS pathway) [catalytic activity: UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate]. Alpha, alpha-trehalose-phosphate synthase [UDP-forming] OtsA (trehalose-6-phosphate synthase) (UDP-glucose-glucosephosphate glucosyltransferase) (trehalosephosphate-UDP glucosyltransferase) (trehalose-6-phosphate synthetase) (trehalose-phosphate synthase) (trehalose-phosphate synthetase) (transglucosylase) (trehalosephosphate-UDP glucosyl transferase) Rv3490, (MTCY13E12.43), len: 500 aa. otsA, alpha, alpha-trehalose-phosphate synthase (see citations below), equivalent to Q50167|OTSA|ML2254 probable trehalose-phosphate synthase from Mycobacterium leprae (498 aa), FASTA scores: opt: 2706, E(): 1.6e-166, (80.3% identity in 497 aa overlap). Also similar to others e.g. Q92410|TPS1_CANAL from Candida albicans (Yeast) (478 aa), FASTA scores: opt: 895, E(): 4.9e-50, (37.15% identity in 479 aa overlap); Q00764|TPS1_YEASTTPS1|CIF1|BYP1|FDP1|GGS1|GLC6|YBR126c|YBR0922 from Saccharomyces cerevisiae (Baker's yeast) (495 aa), FASTA scores: opt: 847, E(): 6.2e-47, (36.1% identity in 490 aa overlap); BAB48232|MLL0691 from Rhizobium loti (Mesorhizobium loti) (520 aa), FASTA scores: opt: 884, E(): 2.7e-49, (36.2% identity in 478 aa overlap); etc. Equivalent to AAK47953 from Mycobacterium tuberculosis strain CDC1551 (478 aa) but longer 22 aa. P9WN11 No virulence, detoxification, adaptation O06353 P9WN11 GO:0005992,GO:0016757 2.4.1.- P9WN11 NC_000962.3 Mycobrowser_v5 CDS 3876052 3876822 . - 0 Rv3455c truA Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAS [catalytic activity: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H(2)O]. Probable tRNA pseudouridine synthase a TruA (pseudouridylate synthase I) (pseudouridine synthase I) (uracil hydrolyase) Rv3455c, (MTCY13E12.08c), len: 256 aa. Probable truA, pseudouridine synthase A, equivalent to Q9X796|TRUA_MYCLE|ML1955|MLCB1222.25c tRNA pseudouridine synthase a from Mycobacterium leprae (249 aa), FASTA scores: opt: 1345, E(): 3.2e-80, (77.25% identity in 246 aa overlap). Also highly similar to others e.g. O86776|TRUA_STRCO|SC6G4.09 from Streptomyces coelicolor (284 aa), FASTA scores: opt: 595, E(): 1.7e-31, (49.8% identity in 259 aa overlap); Q9RS37|DR2290 from Deinococcus radiodurans (280 aa), FASTA scores: opt: 383, E(): 1e-17, (41.2% identity in 216 aa overlap); Q9PJT0|TRUA_CHLMU|TC0748 from Chlamydia muridarum (267 aa), FASTA scores: opt: 334, E(): 1.5e-14, (37.65% identity in 231 aa overlap); P07649|TRUA_ECOLI|hist|ASUC|LEUK|B2318 from Escherichia coli strain K12 (270 aa), FASTA scores: opt: 315, E(): 2.5e-13, (33.35% identity in 240 aa overlap); etc. Belongs to the TruA family of pseudouridine synthases. P9WHP9 No information pathways P65846 P9WHP9 GO:0009982,GO:0031119,GO:0003723 5.4.99.12 P9WHP9 NC_000962.3 Mycobrowser_v5 CDS 3876890 3877432 . - 0 Rv3456c rplQ Involved in translation mechanism. 50S ribosomal protein L17 RplQ Rv3456c, (MTCY13E12.09c), len: 180 aa. rplQ, 50S ribosomal protein L17, equivalent to Q9X797|RL17_MYCLE|ML1956|MLCB1222.26c 50S ribosomal protein L17 from Mycobacterium leprae (170 aa), FASTA scores: opt: 874, E(): 9.5e-45, (81.85% identity in 171 aa overlap). Also highly similar to other e.g. O86775|RL17_STRCO|SC6G4.08 from Streptomyces coelicolor (168 aa), FASTA scores: opt: 609, E(): 3.7e-29, (60.0% identity in 170 aa overlap); BAB47931|MLR0326 from Rhizobium loti (Mesorhizobium loti) (143 aa), FASTA scores: opt: 404, E(): 3.7e-17, (49.65% identity in 139 aa overlap); Q9Z9H5|RL17_THETH|RPLQ from Thermus aquaticus (subsp. thermophilus) (118 aa), FASTA scores: opt: 366, E(): 5.5e-15, (53.15% identity in 111 aa overlap); P02416|RL17_ECOLI|RPLQ|B3294 from Escherichia coli strain K12 (127 aa), FASTA scores: opt: 347, E(): 7.6e-14, (50.4% identity in 119 aa overlap); etc. Belongs to the L17P family of ribosomal proteins. P9WHD3 No information pathways P0A5V4 P9WHD3 GO:0003735,GO:0006412,GO:0005840 P9WHD3 NC_000962.3 Mycobrowser_v5 CDS 3878659 3879264 . - 0 Rv3458c rpsD This protein binds directly to 16S ribosomal RNA. 30S ribosomal protein S4 RpsD Rv3458c, (MTCY13E12.11c), len: 201 aa. rpsD, 30S ribosomal protein S4, equivalent to Q9X799|RS4_MYCLE|RPSD|ML1958|MLCB1222.28c 30S ribosomal protein S4 from Mycobacterium leprae (201 aa), FASTA scores: opt: 1271, E(): 2.2e-73, (93.5% identity in 201 aa overlap); and P45811|RS4_MYCBO|RPSD from Mycobacterium bovis (131 aa), FASTA scores: opt: 867, E(): 4.9e-48, (100.0% identity in 130 aa overlap). Also highly similar to others e.g. P81288|RS4_BACST|RPSD from Bacillus stearothermophilus (198 aa), FASTA scores: opt: 665, E(): 4e-35, (52.25% identity in 201 aa overlap); Q9K7Z8|RPSD|BH3209 from Bacillus halodurans (200 aa), FASTA scores: opt: 626, E(): 1.2e-32, (48.75% identity in 203 aa overlap); Q9X1I3|RS4_THEMA|RPSD|TM1473 from Thermotoga maritima (209 aa), FASTA scores: opt: 591, E(): 2e-30, (45.0% identity in 209 aa overlap); etc. Contains ribosomal protein S4 signature (PS00632) and ATP/GTP binding site motif A (PS00017). Belongs to the S4P family of ribosomal proteins. P9WH35 No information pathways O06325 P9WH35 GO:0015935,GO:0003735,GO:0006412,GO:0019843 P9WH35 NC_000962.3 Mycobrowser_v5 CDS 3882834 3883442 . + 0 Rv3465 rmlC Involved in dTDP-L-rhamnose biosynthesis, within the O antigen biosynthesis pathway of lipopolysaccharide biosynthesis: conversion of dTDP-4-keto-6-deoxy-D-glucose to dTDP-4-keto-rhamnose [catalytic activity: dTDP-4-dehydro-6-deoxy-D-glucose = dTDP-4-dehydro-6-deoxy-L-mannose]. dTDP-4-dehydrorhamnose 3,5-epimerase RmlC (dTDP-4-keto-6-deoxyglucose 3,5-epimerase) (dTDP-L-rhamnose synthetase) (thymidine diphospho-4-keto-rhamnose 3,5-epimerase) Rv3465, (MTCY13E12.18), len: 202 aa. RmlC (alternate gene name: rfbC), dTDP-4-dehydrorhamnose 3,5-epimerase (see citations below), nearly identical to O33170|RMLC RMLC protein from Mycobacterium tuberculosis (203 aa), FASTA scores: opt: 1171, E(): 2.6e-71, (89.5% identity in 200 aa overlap) (previously known as rfbC). Equivalent to Q9X7A4|RMLC|ML1965 putative dTDP-4-dehydrorhamnose 3,5-epimerase from Mycobacterium leprae (202 aa), FASTA scores: opt: 1072, E(): 1.1e-64, (75.4% identity in 199 aa overlap). Also highly similar to others e.g. Q9F8S7|CUMY from Streptomyces rishiriensis (198 aa), FASTA scores: opt: 671, E(): 7e-38, (51.3% identity in 193 aa overlap); Q9L6C5 from Streptomyces antibioticus (202 aa), FASTA scores: opt: 665, E(): 1.8e-37, (49.25% identity in 197 aa overlap); P29783|STRM_STRGR from Streptomyces griseus (200 aa), FASTA scores: opt: 608, E(): 1.2e-33, (49.25% identity in 201 aa overlap); Q54265|STRM from Streptomyces glaucescens (200 aa), FASTA scores: opt: 603, E(): 2.5e-33, (46.7% identity in 197 aa overlap); etc. Also highly similar to Q9S4D4|TYLJ putative NDP-hexose 3-epimerase from Streptomyces fradiae (205 aa), FASTA scores: opt: 625, E(): 8.6e-35, (45.9% identity in 194 aa overlap). P9WH11 No intermediary metabolism and respiration 1PM7,1UPI,2IXC O06330 P9WH11 GO:0009103,GO:0008830 5.1.3.13 P9WH11 NC_000962.3 Mycobrowser_v5 CDS 3883525 3884193 . + 0 Rv3466 Rv3466 Unknown Conserved hypothetical protein Rv3466, (MTCY13E12.19), len: 222 aa. Conserved hypothetical ORF in REP13E12 repeat, but extending 5' of repeat. Has segment of identity to other REP13E12 ORF's e.g. MTCY336.16, MTCI65.15c, MTCY09F9.19, cMTCY251.14c. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WKY1 No insertion seqs and phages P9WKY1 NC_000962.3 Mycobrowser_v5 CDS 3883964 3884917 . + 0 Rv3467 Rv3467 Unknown Conserved hypothetical protein Rv3467, (MTCY13E12.20), len: 317 aa. Conserved hypothetical ORF in REP13E12 repeat, identical to ORF's from other REP13E12 copies e.g. MTCY251.13c, MTCI65.15c, MTCY09F9.19, cMTCY336.17. Also identical to Mycobacterium bovis Q50655 hypothetical 34.6 kDa protein (317 aa) in identical repeat. Q50655 No insertion seqs and phages Q50655 Q50655 NC_000962.3 Mycobrowser_v5 CDS 3884975 3886069 . - 0 Rv3468c Rv3468c Possibly involved in dTDP-L-rhamnose biosynthesis [catalytic activity: dTDP-glucose = dTDP-4-dehydro-6-deoxy-D-glucose + H(2)O]. Possible dTDP-glucose 4,6-dehydratase Rv3468c, (MTCY13E12.21c), len: 364 aa. Possible dTDP-glucose-4,6-dehydratase, but experimental study shown that the purified protein didn't have dTDP-glucose dehydratase (rmlB) activity (see Ma et al., 2001). Similar to others e.g. O08246|MTME from Streptomyces argillaceus (331 aa), FASTA scores: opt: 238, E(): 1.2e-07, (29.65% identity in 344 aa overlap); Q9LFG7|F4P12_220 from Arabidopsis thaliana (Mouse-ear cress) (433 aa), FASTA scores: opt: 237, E(): 1.8e-07, (27.25% identity in 308 aa overlap); Q9LZI2|F26K9_260 from Arabidopsis thaliana (Mouse-ear cress) (445 aa), FASTA scores: opt: 225, E(): 1e-06, (25.95% identity in 335 aa overlap); etc. Also similar to various enzymes and hypothetical unknowns proteins e.g. BAB48655|MLL1234 UDP-glucose 4-epimerase from Rhizobium loti (Mesorhizobium loti) (307 aa), FASTA scores: opt: 757, E(): 4.6e-40, (43.4% identity in 302 aa overlap). First start taken, alternative at 17080 in cSCYY13E12 suggested by similarity. Note that previously known as rmlB3 (see Ma et al., 2001). Q6MWX3 No intermediary metabolism and respiration Q6MWX3 Q6MWX3 GO:0050662,GO:0008460,GO:0044237 4.2.1.46 Q6MWX3 NC_000962.3 Mycobrowser_v5 CDS 3891105 3892091 . + 0 Rv3475 Rv3475 Involved in the transposition of the insertion sequence IS6110. Possible transposase for insertion element IS6110 [second part] Rv3475, (MTCY13E12.28), len: 328 aa. Probable transposase subunit for IS6110. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv3474 and Rv3475, the sequence UUUUAAAG (directly upstream of Rv3475) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (- 18 aa). P9WKH9 No insertion seqs and phages P0C5G8 P9WKH9 GO:0015074,GO:0006310,GO:0032196,GO:0003677 P9WKH9 NC_000962.3 Mycobrowser_v5 CDS 3887144 3888802 . - 0 Rv3470c ilvB2 Involved in valine and isoleucine biosynthesis (at the first step) [catalytic activity: 2-acetolactate + CO(2) = 2-pyruvate]. Probable acetolactate synthase (large subunit) IlvB2 (AHAS) (acetohydroxy-acid synthase large subunit) (ALS) Rv3470c, (MTCY13E12.23c), len: 552 aa. Probable ilvB2, acetolactate synthase large subunit, similar to others e.g. P73913|ILVG|SLR2088 from Synechocystis sp. strain PCC 6803 (621 aa), FASTA scores: opt: 779, E(): 4.5e-39, (30.7% identity in 567 aa overlap); O78518|ILVB_GUITH from Guillardia theta (Cryptomonas phi) (575 aa), FASTA scores: opt: 742, E(): 6.9e-37, (28.8% identity in 566 aa overlap); Q59950|ILVX from Spirulina platensis (612 aa), FASTA scores: opt: 715, E(): 3e-35, (28.45% identity in 569 aa overlap); etc. Contains thiamine pyrophosphate enzymes signature (PS00187). O06335 No intermediary metabolism and respiration O06335 O06335 GO:0000287,GO:0030976,GO:0003984 2.2.1.6 O06335 NC_000962.3 Mycobrowser_v5 CDS 3888808 3889341 . - 0 Rv3471c Rv3471c Function unknown Conserved hypothetical protein Rv3471c, (MTCY13E12.24c), len: 177 aa. Conserved hypothetical protein, similar to Q59013|MJ1618 hypothetical protein from Methanococcus jannaschii (125 aa), FASTA scores: opt: 262, E(): 1.2e-09, (39.05% identity in 105 aa overlap); and O26452|MTH352 conserved protein from Methanobacterium thermoautotrophicum (131 aa), FASTA scores: opt: 222, E(): 3.8e-07, (35.05% identity in 117 aa overlap). Equivalent to AAK47934 from Mycobacterium tuberculosis strain CDC1551 (184 aa) but shorter 7 aa. O06336 No conserved hypotheticals O06336 O06336 GO:0045735 O06336 NC_000962.3 Mycobrowser_v5 CDS 3889362 3889868 . + 0 Rv3472 Rv3472 Function unknown Conserved protein Rv3472, (MTCY13E12.25), len: 168 aa. Conserved protein, showing some similarity to other proteins e.g. Q9ZAT9|DPSH daunorubicin biosynthesis enzyme from Streptomyces peucetius (194 aa), FASTA scores: opt: 181, E(): 6.8e-05, (30.7% identity in 127 aa overlap); Q53879 DAUH/E from Streptomyces sp. C5 (151 aa), FASTA scores: opt: 168, E(): 0.00038, (29.25% identity in 127 aa overlap); and Q9L4U3|AKNV from Streptomyces galilaeus (144 aa), FASTA scores: opt: 122, E(): 0.36, (31.25% identity in 129 aa overlap). I6YG83 No conserved hypotheticals 2CHC I6YG83 I6YG83 NC_000962.3 Mycobrowser_v5 CDS 3901324 3902106 . - 0 Rv3482c Rv3482c Unknown Probable conserved membrane protein Rv3482c, (MTCY13E12.35c), len: 260 aa. Probable conserved membrane protein. N-terminal region shares some similarity with N-terminus of O88067|SCI35.32c putative membrane protein from Streptomyces coelicolor (319 aa), FASTA scores: opt: 155, E(): 0.023, (54.55% identity in 33 aa overlap); and with C-terminus of O06254|Rv3437|MTCY77.09 hypothetical 17.9 KDA protein from Mycobacterium tuberculosis strain H37Rv (alias AAK47883|MT3542.1 from strain CDC1551) (158 aa), FASTA scores: opt: 140, E(): 0.11, (58.8% identity in 34 aa overlap). Some similarity to others e.g. Q9XAN5|SC4C6.05c putative membrane protein from Streptomyces coelicolor (347 aa), FASTA scores: opt: 131, E(): 0.75, (29.4% identity in 221 aa overlap). First start taken. I6YG92 No cell wall and cell processes O06345 I6YG92 I6YG92 NC_000962.3 Mycobrowser_v5 CDS 3890830 3891156 . + 0 Rv3474 Rv3474 Involved in the transposition of the insertion sequence IS6110. Possible transposase for insertion element IS6110 (fragment) Rv3474, (MTCY13E12.27), len: 108 aa. Probable transposase subunit for IS6110. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv3474 and Rv3475, the sequence UUUUAAAG (directly upstream of Rv3475) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Belongs to the transposase family 8. P9WKH5 No insertion seqs and phages P0C5G9 P9WKH5 GO:0004803,GO:0006313,GO:0003677 P9WKH5 NC_000962.3 Mycobrowser_v5 CDS 3895820 3898885 . + 0 Rv3479 Rv3479 Unknown Possible transmembrane protein Rv3479, (MTCY13E12.32), len: 1021 aa. Possible transmembrane protein, with hydrophobic stretches at C-terminus. Start changed since first submission (-54 aa). Alternative nucleotide at position 3896340 (T->G; L174R) has been observed. O06342 No cell wall and cell processes O06342 O06342 GO:0006629,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 3898909 3900402 . - 0 Rv3480c Rv3480c May be involved in synthesis of triacylglycerol Possible triacylglycerol synthase (diacylglycerol acyltransferase) Rv3480c, (MTCY13E12.33c), len: 497 aa. Possible triacylglycerol synthase (See Daniel et al., 2004), similar to many from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. O69701|Y1D4_MYCTU|Rv3734c|MT3839|MTV025.082c (454 aa), FASTA scores: opt: 520, E(): 2e-23, (39.95% identity in 488 aa overlap); Q10554|Y895_MYCTU|Rv0895|MTCY31.23 (505 aa), FASTA scores: opt: 434, E(): 2.7e-18, (34.2% identity in 497 aa overlap); AAK45165|MT0919 (520 aa), FASTA scores: opt: 434, E(): 2.7e-18, (34.2% identity in 497 aa overlap); etc. Also similar to Q9X7A8|MLCB1610.05|ML1244 conserved membrane protein from Mycobacterium leprae (491 aa), FASTA scores: opt: 272, E(): 1e-08, (28.85% identity in 485 aa overlap); and Q9RIU8|CM11.13c hypothetical 47.1 KDA protein from Streptomyces coelicolor (446 aa), FASTA scores: opt: 254, E(): 1.1e-07, (30.4% identity in 497 aa overlap). Seems to belong to the UPF0089 family. P9WKA7 No lipid metabolism O06343 P9WKA7 GO:0006071,GO:0008610,GO:0004144 2.3.1.20 NC_000962.3 Mycobrowser_v5 CDS 3903078 3904616 . + 0 Rv3484 cpsA Unknown Possible conserved protein CpsA Rv3484, (MTCY13E12.37), len: 512 aa. Possible cpsA, hypothetical protein, equivalent to Q50160|CPSA|ML2247 hypothetical protein CPSA from Mycobacterium leprae (516 aa), FASTA scores: opt: 2557, E(): 1.6e-143, (74.9% identity in 518 aa overlap); and with good similarity to Q9CCK9|ML0750 hypothetical protein from Mycobacterium leprae (489 aa), FASTA scores: opt: 855, E(): 4.6e-43, (34.45% identity in 502 aa overlap). Also similar (or with similarity) to hypothetical proteins from Mycobacterium tuberculosis: P96872|Rv3267|MTCY71.07 (498 aa), FASTA scores: opt: 928, E(): 2.3e-47, (37.35% identity in 498 aa overlap); and O53834|Rv0822c|MTV043.14c (684 aa), FASTA scores: opt: 425, E(): 1.5e-17, (26.15% identity in 524 aa overlap). Shows also similarity with various bacterial proteins e.g. Q9KZK0|SCE34.26 conserved hypothetical protein from Streptomyces coelicolor (507 aa), FASTA scores: opt: 329, E(): 5.3e-12, (28.85% identity in 478 aa overlap); Q9K4E6|2SC6G5.02 conserved hypothetical protein, possible membrane protein, from Streptomyces coelicolor (382 aa), FASTA scores: opt: 305, E(): 1.1e-10, (29.8% identity in 386 aa overlap); O69850|SC1C3.08c putative transcriptional regulator from Streptomyces coelicolor (366 aa), FASTA scores: opt: 304, E(): 1.2e-10, (29.6% identity in 395 aa overlap); Q9KZK3|SCE34.23 putative transcriptional regulator from Streptomyces coelicolor (396 aa), FASTA scores: opt: 296, E(): 3.8e-10, (31.25% identity in 349 aa overlap); AAK43602|CPSA CPSA protein from Streptococcus agalactiae (485 aa), FASTA scores: opt: 250, E(): 2.4e-07, (30.25% identity in 162 aa overlap); etc. Predicted to be an outer membrane protein (See Song et al., 2008). O06347 No conserved hypotheticals O06347 O06347 O06347 NC_000962.3 Mycobrowser_v5 CDS 3904622 3905566 . - 0 Rv3485c Rv3485c Function unknown; supposed to be involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv3485c, (MTCY13E12.38c), len: 314 aa. Probable short-chain dehydrogenase/reductase, similar, but longer 41 aa, to P71824|Rv0769|MTCY369.14 putative short-chain type dehydrogenase/reductase CY369.14 from Mycobacterium tuberculosis (248 aa), FASTA scores: opt: 462, E(): 1.8e-19, (34.0% identity in 253 aa overlap). Also similar to various dehydrogenases e.g. P25529|HDHA_ECOLI|HSDH|B1619 NAD-dependent 7 alpha-hydroxysteroid dehydrogenase (SDR family) from Escherichia coli strain K12 (alias BAB35750|ECS2327 or AAG56608|HDHA for strain O157:H7) (255 aa), FASTA scores: opt: 462, E(): 1.8e-19, (34.7% identity in 248 aa overlap); Q9FD15|RUBG putative reductase (SDR family) from Streptomyces collinus (249 aa), FASTA scores: opt: 446, E(): 1.5e-18, (36.1% identity in 255 aa overlap); BAB51974|MLL5540 putative dehydrogenase from Rhizobium loti (Mesorhizobium loti) (253 aa), FASTA scores: opt: 442, E(): 2.5e-18, (36.25% identity in 251 aa overlap); Q08632|SDR1_PICAB short-chain type dehydrogenase/reductase (SDR family) from Picea abies (Norway spruce) (Picea excelsa) (271 aa), FASTA scores: opt: 441, E(): 3.1e-18, (32.3% identity in 260 aa overlap); Q9A326|CC3380 2-deoxy-D-gluconate 3-dehydrogenase from Caulobacter crescentus (260 aa), FASTA scores: opt: 436, E(): 5.7e-18, (32.8% identity in 253 aa overlap); Q16698|DECR_HUMAN 2,4-dienoyl-CoA reductase, mitochondrial precursor from Homo sapiens (Human) (335 aa), FASTA scores: opt: 430, E(): 1.5e-17, (30.4% identity in 306 aa overlap); etc. Contains short-chain alcohol dehydrogenase family signature (PS00061). Belongs to the short-chain dehydrogenases/reductases family (SDR). O06348 No intermediary metabolism and respiration O06348 O06348 GO:0055114,GO:0016491 1.-.-.- O06348 NC_000962.3 Mycobrowser_v5 CDS 4184012 4184512 . - 0 Rv3733c Rv3733c Function unknown Conserved hypothetical protein Rv3733c, (MTV025.081c), len: 166 aa. Conserved hypothetical protein, highly similar to Q9FCB0|2SCG58.03 putative mutt-like protein from Streptomyces coelicolor (153 aa), FASTA scores: opt: 541, E(): 7.2e-29, (52.7% identity in 148 aa overlap); and BAB49143|MLR1881 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (156 aa), FASTA scores: opt: 526, E(): 7.2e-28, (52.65% identity in 150 aa overlap). O69700 No conserved hypotheticals O69700 O69700 GO:0016787 O69700 NC_000962.3 Mycobrowser_v5 CDS 3910465 3910947 . - 0 Rv3492c Rv3492c Unknown Conserved hypothetical Mce associated protein Rv3492c, (MTCY13E12.45c), len: 160 aa. Conserved hypothetical Mce-associated protein, showing some similarity to hypothetical Mycobacterium tuberculosis proteins e.g. O53974|Rv1973|MTV051.11 (near Mce operon 3) (160 aa), FASTA scores: opt: 214, E(): 2.6e-07, (25.3% identity in 154 aa overlap); and Q11032|YD62_MYCTU|Rv1362c|MT1407|MTCY02B10.26c (220 aa), FASTA scores: opt: 187, E(): 2e-05, (23.4% identity in 154 aa overlap). Contains lipocalin signature at C-terminus (PS00213). Predicted to be an outer membrane protein (See Song et al., 2008). I6YGA5 No conserved hypotheticals O06355 I6YGA5 I6YGA5 NC_000962.3 Mycobrowser_v5 CDS 3955550 3956260 . + 0 Rv3519 Rv3519 Unknown Unknown protein Rv3519, (MTV023.26), len: 236 aa (start uncertain). Unknown protein. The C-terminal end is highly similar to N-terminal end of AAK47980|MT3620 hypothetical 7.8 KDA protein from Mycobacterium tuberculosis strain CDC1551 (73 aa), FASTA scores: opt: 279, E(): 9.4e-12, (95.65% identity in 46 aa overlap). O53564 No conserved hypotheticals O53564 O53564 GO:0016831 O53564 NC_000962.3 Mycobrowser_v5 CDS 3981045 3981980 . - 0 Rv3542c Rv3542c Function unknown Conserved hypothetical protein Rv3542c, (MTCY03C7.14), len: 311 aa. Hypothetical protein, showing some similarity to other e.g. Q58947|MJ1552 from Methanococcus jannaschii (141 aa) FASTA scores: opt: 177, E(): 0.00065, (46.65% identity in 60 aa overlap); BAB59276|TVG0142586 from Thermoplasma volcanium (135 aa), FASTA scores: opt: 175, E(): 0.00083, (35.65% identity in 87 aa overlap); Q9HI85|TA1457 from Thermoplasma acidophilum (135 aa), FASTA scores: opt: 162, E(): 0.0052, (31.8% identity in 107 aa overlap); etc. I6YGF8 No conserved hypotheticals P71859 I6YGF8 I6YGF8 NC_000962.3 Mycobrowser_v5 CDS 3907667 3907990 . + 0 Rv3488 Rv3488 Function unknown Conserved hypothetical protein Rv3488, (MTCY13E12.41), len: 107 aa. Hypothetical protein, similar to various bacterial proteins e.g. O28730|AF1542 conserved hypothetical protein from Archaeoglobus fulgidus (101 aa), FASTA scores: opt: 321, E(): 6.4e-15, (50.55% identity in 87 aa overlap); O50207 SQ1_IV (fragment) from Rhodococcus erythropolis (59 aa), FASTA scores: opt: 298, E(): 1.4e-13, (71.2% identity in 59 aa overlap); Q9KFB0|BH0575 BH0575 protein from Bacillus halodurans (102 aa), FASTA scores: opt: 294, E(): 4.1e-13, (43.15% identity in 95 aa overlap); etc. Also similar to Mycobacterium tuberculosis P71704|Rv0047c|MTCY21D4.10c (180 aa) (37.8% identity in 82 aa overlap). I6X7F9 No conserved hypotheticals O06351 I6X7F9 I6X7F9 NC_000962.3 Mycobrowser_v5 CDS 3911675 3913369 . - 0 Rv3494c mce4F Unknown, but thought to be involved in host cell invasion. Predicted to be involved in lipid catabolism. Mce-family protein Mce4F Rv3494c, (MTV023.01c), len: 564 aa. Mce4F; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), similar to Mycobacterium tuberculosis proteins O07418|Rv0174|MTCI28.14|mce1F (515 aa); O07784|Rv0594|MTCY19H5.28c|mce2F (516 aa); and O53972|Rv1971|MTV051.09|mce3F (437 aa). Also similar to others e.g. Q9CD09|MCE1F|ML2594 putative secreted protein from Mycobacterium leprae (516 aa), FASTA scores: opt: 1040, E(): 3.6e-31, (35.9% identity in 529 aa overlap); Q9F361|SC8A2.02c putative secreted protein from Streptomyces coelicolor (433 aa), FASTA scores: opt: 570, E(): 3.7e-14, (30.8% identity in 458 aa overlap); etc. Has hydrophobic stretch, possibly a signal peptide at the N-terminus. Predicted to be an outer membrane protein (See Song et al., 2008). I6YC95 No virulence, detoxification, adaptation O53539 I6YC95 I6YC95 NC_000962.3 Mycobrowser_v5 CDS 4093632 4093946 . + 0 Rv3652 PE_PGRS60 Function unknown PE-PGRS family-related protein PE_PGRS60 Rv3652, (MTV025.001A), len: 104 aa. PE_PGRS60, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), similar at N-terminal end with many e.g. P56877|Y278_MYCTU|Rv0278c|MTV035.06c (957 aa) FASTA scores: opt: 242, E(): 3e-09, (77.35% identity in 53 aa overlap). Originally annotated as the first part of a PE-PGRS family protein (Rv3653/PE_PGRS61 being the second part) but more similar to a PE family protein. Length extended since first submission (+50 aa). Q6MWV1 No PE/PPE Q6MWV1 Q6MWV1 NC_000962.3 Mycobrowser_v5 CDS 3913380 3914534 . - 0 Rv3495c lprN Unknown, but thought to be involved in host cell invasion. Predicted to be involved in lipid catabolism. Possible Mce-family lipoprotein LprN (Mce-family lipoprotein Mce4E) Rv3495c, (MTV023.02c), len: 384 aa. Possible lprN (alternate gene name: mce4E), lipoprotein which belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07417|LPRK|Rv0173|MTCI28.13|mce1E (390 aa); O07785|LPRL|Rv0593|MTCY19H5.29|mce2E (402 aa); and O53971|LPRM|Rv1970|MTV051.08|mce3E (377 aa). Also similar to others e.g. Q9F360|SC8A2.03c putative secreted protein from Streptomyces coelicolor (413 aa), FASTA scores: opt: 656, E(): 2.2e-32, (37.55% identity in 317 aa overlap); Q9CD10|LPRK|ML2593 putative lipoprotein from Mycobacterium leprae (392 aa), FASTA scores: opt: 616, E(): 5.5e-30, (28.95% identity in 373 aa overlap); etc. Contains possible signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. I6Y3P1 No cell wall and cell processes O53540 I6Y3P1 I6Y3P1 NC_000962.3 Mycobrowser_v5 CDS 3992685 3994742 . + 0 Rv3554 fdxB Function unknown; C-terminus probably involved in electron transfer in one or several metabolic reactions. Possible electron transfer protein FdxB Rv3554, (MTCY06G11.01, MTCY03C7.01c), len: 685 aa. Possible fdxB, two-domain protein, with ferredoxin reductase electron transfer component in C-terminal part and unknown function in N-terminal part. Indeed, N-terminal end is similar to O85832 hypothetical 36.1 KDA protein from Sphingomonas aromaticivorans strain F199 (catabolic plasmid pNL1) (309 aa), FASTA scores: opt: 615, E(): 2.5e-30, (33.1% identity in 311 aa overlap); and P73428|SLL1468 hypothetical 36.2 KDA protein from Synechocystis sp. strain PCC 6803 (312 aa), FASTA scores: opt: 317, E(): 4.5e-12, (30.2% identity in 268 aa overlap). And C-terminal end is similar to Q9F9U6|PAAE protein involved in aerobic phenylacetate metabolism from Azoarcus evansii (360 aa), FASTA scores: opt: 935, E(): 7e-50, (43.85% identity in 351 aa overlap); CAC44653|PAAE|SCBAC17A6.08 putative phenylacetic acid degradation NADH oxidoreductase from Streptomyces coelicolor (368 aa), FASTA scores: opt: 93, E(): 9.5e-50, (41.95% identity in 372 aa overlap); Q9FA57|PACI ferredoxin from Azoarcus evansii (360 aa), FASTA scores: opt: 925, E(): 2.9e-49, (43.3% identity in 351 aa overlap); P76081|PAAE_ECOLI|B1392 probable phenylacetic acid degradation NADH oxidoreductase from Escherichia coli strains K12 and W (356 aa), FASTA scores: opt: 910, E(): 2.4e-48, (43.05% identity in 353 aa overlap); Q9APJ6|PAAE electron transfer protein (fragment) from Hyphomicrobium chloromethanicum (241 aa), FASTA scores: opt: 404, E(): 1.7e-17, (35.45% identity in 234 aa overlap); BAB51608|MLL5100 ferredoxin from Rhizobium loti (Mesorhizobium loti) (365 aa), FASTA scores: opt: 316, E(): 5.8e-12, (28.95% identity in 349 aa overlap); etc. C-terminus also similar to P96853|Rv3571|MTCY06G11.18 putative electron transfer protein from Mycobacterium tuberculosis (358 aa), FASTA scores: opt: 450, E(): 3.6e-20, (32.95% identity in 358 aa overlap). Contains PS00197 2Fe-2S ferredoxins, iron-sulfur binding region signature. Belongs to the 2FE2S plant-type ferredoxin family. Cofactor: binds a 2FE-2S cluster (by similarity). P71846 No intermediary metabolism and respiration P71846 P71846 GO:0009055,GO:0006629,GO:0046872,GO:0055114,GO:0016491,GO:0051537 1.-.-.- P71846 NC_000962.3 Mycobrowser_v5 CDS 3915883 3916956 . - 0 Rv3497c mce4C Unknown, but thought to be involved in host cell invasion. Predicted to be involved in lipid catabolism. Mce-family protein Mce4C Rv3497c, (MTV023.04c), len: 357 aa. Mce4C; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07415|R0171|MTCI28.11|mce1C (515 aa); O07787|Rv0591|MTCY19H5.31|mce2C (481 aa); and O53969|Rv1968|MTV051.06|mce3C (410 aa). Also similar to others e.g. Q9F358|SC8A2.05c putative secreted protein from Streptomyces coelicolor (351 aa), FASTA scores: opt: 658, E(): 1.1e-30, (33.95% identity in 318 aa overlap); Q9CD12|MCE1C|ML2591 putative secreted protein from Mycobacterium leprae (519 aa), FASTA scores: opt: 555, E(): 1.2e-24, (28.35% identity in 328 aa overlap); etc. Hydrophobic region at N-terminus. Predicted to be an outer membrane protein (See Song et al., 2008). I6YGB1 No virulence, detoxification, adaptation O53542 I6YGB1 I6YGB1 NC_000962.3 Mycobrowser_v5 CDS 3916946 3917998 . - 0 Rv3498c mce4B Unknown, but thought to be involved in host cell invasion. Predicted to be involved in lipid catabolism. Mce-family protein Mce4B Rv3498c, (MTV023.05c), len: 350 aa. Mce4B; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07414|Rv0170|MTCI28.10|mce1B (346 aa); O07788|Rv0590|MTCY19H5.32c|mce2B (275 aa); and O53968|Rv1967|MTV051.05|mce3B (342 aa). Also similar to others e.g. Q9CD13|MCE1B|ML2590 putative secreted protein from Mycobacterium leprae (346 aa), FASTA scores: opt: 803, E(): 6.1e-41, (41.05% identity in 346 aa overlap); Q9F357|SC8A2.06c putative secreted protein from Streptomyces coelicolor (354 aa), FASTA scores: opt: 624, E(): 3.4e-30, (32.55% identity in 338 aa overlap); etc. Hydrophobic region at N-terminus. Predicted to be an outer membrane protein (See Song et al., 2008). I6X7G8 No virulence, detoxification, adaptation O53543 I6X7G8 I6X7G8 NC_000962.3 Mycobrowser_v5 CDS 3917998 3919200 . - 0 Rv3499c mce4A Unknown, but thought to be involved in host cell invasion. Predicted to be involved in lipid catabolism. Mce-family protein Mce4A Rv3499c, (MTV023.06c), len: 400 aa. Mce4A; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins P72013|MCE1|Rv0169|MTCI28.09|mce1A (454 aa); O07789|MCE2|Rv0589|MTCY19H5.33c|mce2A (404 aa); and O53967|MCE3|Rv1966|MTV051.04|mce3A (425 aa). Also similar to others e.g. Q9F356|SC8A2.07c putative secreted protein from Streptomyces coelicolor (418 aa), FASTA scores: opt: 619, E(): 7.8e-30, (32.4% identity in 352 aa overlap); Q9S4U5|MCE1 mycobacterial cell entry protein from Mycobacterium bovis BCG (454 aa), FASTA scores: opt: 529, E(): 2.1e-24, (30.35% identity in 448 aa overlap); Q9CD14|MCE1A|ML2589 from Mycobacterium leprae (441 aa), FASTA scores: opt: 515, E(): 1.4e-23, (28.35% identity in 430 aa overlap); etc. Contains a possible N-terminal signal sequence. Note that previously known as mce4. Predicted to be an outer membrane protein (See Song et al., 2008). I6YC99 No virulence, detoxification, adaptation Q7D5E7 I6YC99 I6YC99 NC_000962.3 Mycobrowser_v5 CDS 3919220 3920062 . - 0 Rv3500c yrbE4B Unknown. Predicted to be involved in lipid catabolism. Conserved integral membrane protein YrbE4B. Possible ABC transporter. Rv3500c, (MTV023.07c), len: 280 aa. YrbE4B, conserved integral membrane protein, part of mce4 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07413|Rv0168|MTCI28.08|yrbE1B (289 aa); O07790|Rv0588|MTCY19H5.34|yrbE2B (295 aa); and O53966|Rv1965|MTV051.03|yrbE3B (271 aa). Also highly similar to conserved hypothetical integral membrane proteins of the P45030|YRBE_HAEIN (261 aa) type, e.g. Q9CD15|YRBE1B|ML2588 from Mycobacterium leprae (289 aa), FASTA scores: opt: 973, E(): 1.5e-50, (50.2% identity in 269 aa overlap); P45030|YRBE_HAEIN|HI1086 from Haemophilus influenzae (261 aa), FASTA scores: opt: 270, E(): 6e-11, (25.4% identity in 264 aa overlap); etc. I6Y3P5 No virulence, detoxification, adaptation O53545 I6Y3P5 I6Y3P5 NC_000962.3 Mycobrowser_v5 CDS 4071236 4071580 . + 0 Rv3632 Rv3632 Unknown Possible conserved membrane protein Rv3632, (MTCY15C10.20c), len: 114 aa. Possible conserved membrane protein, equivalent to O69541|MLCB2548.23c|ML0208 hypothetical 12.9 KDA protein (putative membrane protein) from Mycobacterium leprae (113 aa), FASTA scores: opt: 594, E(): 7.1e-35, (82.0% identity in 111 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). I6YGT7 No cell wall and cell processes O06375 I6YGT7 NC_000962.3 Mycobrowser_v5 CDS 3922065 3922256 . - 0 Rv3503c fdxD Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Probable ferredoxin FdxD Rv3503c, (MTV023.10c), len: 63 aa. Probable fdxD, ferredoxin, equivalent to Q9R6Z5|B229_C3_226 hypothetical 9.3 KDA protein from Mycobacterium leprae (83 aa) FASTA scores: opt: 276, E(): 1.8e-13, (75.9% identity in 54 aa overlap). Also similar to several e.g. Q9R6Z5|PHDC from Nocardioides sp. strain KP7 (69 aa), FASTA scores: opt: 177, E(): 2.1e-06, (43.35% identity in 60 aa overlap); Q9X4X8|DITA3 dioxygenase DITA ferredoxin component from Pseudomonas abietaniphila (78 aa), FASTA scores: opt: 166, E(): 1.4e-05, (36.2% identity in 58 aa overlap); P00203|FER_MOOTH from Moorella thermoacetica (Clostridium thermoaceticum) (63 aa), FASTA scores: opt: 157, E(): 5.4e-05, (36.65% identity in 60 aa overlap); P18325|FER2_STRGO|SUBB from Streptomyces griseolus (64 aa) FASTA scores: opt: 157, E(): 5.5e-05, (39.35% identity in 61 aa overlap); etc. Belongs to the bacterial type ferredoxin family. I6X7H4 No intermediary metabolism and respiration O53548 I6X7H4 GO:0005506,GO:0009055 I6X7H4 NC_000962.3 Mycobrowser_v5 CDS 3922471 3923673 . + 0 Rv3504 fadE26 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE26 Rv3504, (MTV023.11), len: 400 aa. Probable fadE26, acyl-CoA dehydrogenase, similar to other acyl-CoA dehydrogenases from Mycobacterium tuberculosis e.g. P71858|FADE29|Rv3543c|MTCY03C7.13 (387 aa) FASTA scores: opt: 1031, E(): 7.5e-59, (46.25% identity in 402 aa overlap); and P95280|FADE17|Rv1934c|MTCY09F9.30 (409 aa), FASTA scores: opt: 617, E(): 3.1e-32, (32.6% identity in 423 aa overlap); etc. Also similar to others e.g. Q9A6G3|CC2131 from Caulobacter crescentus (403 aa) FASTA scores: opt: 710, E(): 3.2e-38, (33.4% identity in 413 aa overlap); Q9I4V2|PA1022 from Pseudomonas aeruginosa (381 aa), FASTA scores: opt: 522, E(): 3.7e-26, (34.1% identity in 358 aa overlap); Q9RJX2|SCF37.29c from Streptomyces coelicolor (393 aa), FASTA scores: opt: 509, E(): 2.6e-25, (34.45% identity in 363 aa overlap); etc. Could belong to the acyl-CoA dehydrogenases family. I6YCA3 No lipid metabolism O53549 I6YCA3 GO:0003995,GO:0055114,GO:0050660 1.3.99.- I6YCA3 NC_000962.3 Mycobrowser_v5 CDS 4000432 4001589 . - 0 Rv3560c fadE30 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE30 Rv3560c, (MTCY06G11.07c), len: 385 aa. Probable fadE30, acyl-CoA dehydrogenase, similar to many e.g. Q9I4V2|PA1022 from Pseudomonas aeruginosa (381 aa), FASTA scores: opt: 845, E(): 1.6e-47, (39.2% identity in 388 aa overlap); Q9A5G9|CC2478 from Caulobacter crescentus (407 aa), FASTA scores: opt: 734, E(): 2.8e-40, (35.5% identity in 386 aa overlap); Q9RJX2|SCF37.29c from Streptomyces coelicolor (393 aa), FASTA scores: opt: 656, E(): 3.2e-35, (37.9% identity in 351 aa overlap); etc. Also similar to acyl-CoA dehydrogenases from Mycobacterium tuberculosis e.g. P95280|FADE17|Rv1934c|MTCY09F9.30 (409 aa), FASTA scores: opt: 939, E(): 1.4e-53, (43.8% identity in 404 aa overlap). Could belong to the acyl-CoA dehydrogenases family. I6Y3V5 No lipid metabolism P96842 I6Y3V5 GO:0003995,GO:0055114,GO:0050660 1.3.99.- I6Y3V5 NC_000962.3 Mycobrowser_v5 CDS 4196171 4196506 . - 0 Rv3746c PE34 Function unknown Probable PE family protein PE34 (PE family-related protein) Rv3746c, (MTV025.094c), len: 111 aa. PE34, Probable member of the Mycobacterium tuberculosis PE family (see citation below), but without the glycine-rich C-terminal part, similar to N-termini of many e.g. O69737|Rv3872|MTV027.07 (99 aa) FASTA scores: opt: 306, E(): 1e-13, (50.5% identity in 99 aa overlap); O53215|Rv2490c|MTV008.46 (1660 aa) FASTA scores: opt: 125, E(): 0.99, (34.25% identity in 111 aa overlap). Also weakly similar to MTV008_46; MTCI418B_6; MTCY130_1; MTY25D10_11; MTCY1A11_25; MTCY21B4_13; MTCY21B4_27; MTCY493_2; MTCY28_25; etc. Q79FA1 No PE/PPE Q79FA1 Q79FA1 NC_000962.3 Mycobrowser_v5 CDS 3926569 3930714 . + 0 Rv3507 PE_PGRS53 Function unknown PE-PGRS family protein PE_PGRS53 Rv3507, (MTV023.14), len: 1381 aa. PE_PGRS53, Member of the Mycobacterium tuberculosis PE protein family, PGRS subfamily of gly-rich proteins (see citation below), similar to others from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. O06810|Rv1450c|MTCY493.04 (1329 aa), FASTA scores: opt: 2173, E(): 1.4e-135, (51.15% identity in 1412 aa overlap). Equivalent to AAK47970 from Mycobacterium tuberculosis strain CDC1551 (1384 aa) but with some minor differences between the proteins. Contains two PS00583 pfkB family of carbohydrate kinases signatures 1. Q6MWW9 No PE/PPE Q6MWW9 Q6MWW9 Q6MWW9 NC_000962.3 Mycobrowser_v5 CDS 3931005 3936710 . + 0 Rv3508 PE_PGRS54 Function unknown PE-PGRS family protein PE_PGRS54 Rv3508, (MTV023.15), len: 1901 aa. PE_PGRS54, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002), similar to others from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. downstream O53559|Rv3514|MTV023.21 (1489 aa), FASTA scores: opt: 6598, E(): 0, (71.05% identity in 1533 aa overlap). Equivalent to AAK47971 from Mycobacterium tuberculosis strain CDC1551 (1384 aa) but shorter 13 aa and with some minor differences between the proteins. Contains five PS00583 pfkB family of carbohydrate kinases signatures 1. O53553 No PE/PPE O53553 O53553 O53553 NC_000962.3 Mycobrowser_v5 CDS 3939617 3941761 . + 0 Rv3511 PE_PGRS55 Function unknown PE-PGRS family protein PE_PGRS55 Rv3511, (MTV023.18), len: 714 aa. PE_PGRS55, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), similar to others from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. AAK47974|MT3615.3 (1217 aa) FASTA scores: opt: 2563, E(): 1.5e-94, (59.65% identity in 773 aa overlap); and upstream O53553|Rv3508|MTV023.15 (1901 aa), FASTA scores: opt: 2455, E(): 3.9e-90, (60.4% identity in 737 aa overlap); etc. Contains PS00583 pfkB family of carbohydrate kinases signature 1. Q6MWW8 No PE/PPE Q6MWW8 Q6MWW8 Q6MWW8 NC_000962.3 Mycobrowser_v5 CDS 3938421 3939257 . - 0 Rv3510c Rv3510c Function unknown Conserved protein Rv3510c, (MTV023.17), len: 278 aa. Conserved protein, similar to Q50662|Rv2303c|MTCY339.06 hypothetical 34.6 KDA protein from Mycobacterium tuberculosis (307 aa), FASTA scores: opt: 416, E(): 1.2e-19, (35.7% identity in 255 aa overlap). Middle of the putative protein highly similar to N-terminal end of Q49860|B229_C2_182 hypothetical 11.0 KDA protein from Mycobacterium leprae (95 aa), FASTA scores: opt: 304, E(): 7.9e-13, (83.65% identity in 55 aa overlap). Also some similarity with other bacterial proteins e.g. P95886 ORF C02006 from Sulfolobus solfataricus (269 aa), FASTA scores: opt: 293, E(): 9.6e-12, (31.3% identity in 198 aa overlap); Q9XDF3|NONC NONC protein from Streptomyces griseus subsp. griseus (317 aa), FASTA scores: opt: 270, E(): 3.4e-10, (29.95% identity in 227 aa overlap); Q54229|NONR macrotetrolide antibiotic-resistance protein from Streptomyces griseus (347 aa), FASTA scores: opt: 270, E(): 3.6e-10, (29.95% identity in 227 aa overlap); etc. I6Y3Q7 No conserved hypotheticals O53555 I6Y3Q7 GO:0008152,GO:0003824 I6Y3Q7 NC_000962.3 Mycobrowser_v5 CDS 3945092 3945748 . - 0 Rv3513c fadD18 Function unknown, but involved in lipid degradation. Probable fatty-acid-CoA ligase FadD18 (fragment) (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv3513c, (MTV023.20c), len: 218 aa (Start uncertain). Probable fadD18, fatty-acid-CoA synthetase (C-terminal fragment), almost identical to C-terminal end of downstream O53560|FADD19|Rv3515c|MTV023.22c, probably result of partial gene duplication. Also similar at the C-terminus to other fatty-acid-CoA synthetases e.g. Q9EXL2|FADD from Streptomyces griseus (540 aa), FASTA scores: opt: 586, E(): 1.2e-28, (52.45% identity in 185 aa overlap); AAB87139|MIG medium chain acyl-CoA synthetase precursor from Mycobacterium avium (550 aa), FASTA scores: opt: 506, E(): 9.5e-24, (50.0% identity in 150 aa overlap); Q9A7C3|CC1801 putative 4-coumarate--CoA ligase from Caulobacter crescentus (561 aa), FASTA scores: opt: 430, E(): 4.4e-19, (45.75% identity in 153 aa overlap); Q9KDT0|BH1131 acid-CoA ligase from Bacillus halodurans (546 aa), FASTA scores: opt: 338, E(): 1.9e-13, (38.05% identity in 142 aa overlap); Q9RTR4|DR1692 long-chain fatty acid--CoA ligase from Deinococcus radiodurans (584 aa), FASTA scores: opt: 331, E(): 5.3e-13, (35.15% identity in 145 aa overlap); etc. I6YGC8 No lipid metabolism O53558 I6YGC8 GO:0008152,GO:0016874 6.2.1.- I6YGC8 NC_000962.3 Mycobrowser_v5 CDS 3960755 3961786 . + 0 Rv3524 Rv3524 Unknown Probable conserved membrane protein Rv3524, (MTCY03C7.32c), len: 343 aa. Probable conserved membrane protein, showing some similarity to C-terminal part of putative Mycobacterium tuberculosis proteins O05871|P95308|PKND_MYCTU|Rv0931c|MT0958|MTCY08C9.08 serine-threonine protein kinase PknD (664 aa) FASTA scores: opt: 727, E(): 8.3e-36, (45.3% identity in 298 aa overlap); O53893|Rv0980c|MTV044.08c PGRS-family protein (457 aa), FASTA scores: opt: 208, E(): 4.4e-05, (33.75% identity in 166 aa overlap); and O53891|Rv0978c|MTV044.06c PGRS-family protein (331 aa) FASTA scores: opt: 153, E(): 0.062, (30.75% identity in 117 aa overlap). Contains PS00237 G-protein coupled receptors signature. I6X7J6 No cell wall and cell processes O86333 I6X7J6 I6X7J6 NC_000962.3 Mycobrowser_v5 CDS 3969343 3970563 . + 0 Rv3532 PPE61 Function unknown PPE family protein PPE61 Rv3532, (MTCY03C7.24c), len: 406 aa. PPE61, Member of the Mycobacterium tuberculosis PPE protein family, similar to many, e.g. O53956|Rv1807|MTV049.29 (403 aa), FASTA scores: opt: 954, E(): 1.1e-43, (44.1% identity in 417 aa overlap) P9WHX9 No PE/PPE Q6MWW4 P9WHX9 P9WHX9 NC_000962.3 Mycobrowser_v5 CDS 4019262 4020128 . + 0 Rv3577 Rv3577 Function unknown Conserved hypothetical protein Rv3577, (MTCY06G11.24), len: 288 aa (other start sites possible upstream; equivalent to AAK48041 from Mycobacterium tuberculosis strain CDC1551 (379 aa) but shorter 91 aa). Hypothetical protein, showing some similarity to Q9RI88|SCJ11.16c hypothetical 37.9 KDA protein from Streptomyces coelicolor (349 aa) FASTA scores: opt: 285, E(): 1.5e-10, (27.45% identity in 266 aa overlap). P96859 No conserved hypotheticals P96859 GO:0016787 P96859 NC_000962.3 Mycobrowser_v5 CDS 3945794 3950263 . + 0 Rv3514 PE_PGRS57 Function unknown PE-PGRS family protein PE_PGRS57 Rv3514, (MTV023.21), len: 1489 aa. PE_PGRS57, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), similar to others from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. AAK47971 (1715 aa) FASTA scores: opt: 6940, E(): 0, (67.0% identity in 1713 aa overlap); and upstream O53553|YZ08_MYCTU|Rv3508|MTV023.15 (1901 aa), FASTA scores: opt: 6598,E(): 0, (71.05% identity in 1533 aa overlap). Contains two PS00583 pfkB family of carbohydrate kinases signatures 1. Q6MWW6 No PE/PPE Q6MWW6 Q6MWW6 Q6MWW6 NC_000962.3 Mycobrowser_v5 CDS 3950824 3952470 . - 0 Rv3515c fadD19 Function unknown, but involved in lipid degradation. Fatty-acid-CoA ligase FadD19 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv3515c, (MTV023.22c), len: 548 aa. fadD19, fatty-acid-CoA synthetase, similar (or with similarity) to many e.g. Q9EXL2|FADD FADD protein from Streptomyces griseus (540 aa), FASTA scores: opt: 1449, E(): 1.5e-81, (46.0% identity in 535 aa overlap); AAB87139|MIG medium chain acyl-CoA synthetase precursor from Mycobacterium avium (550 aa), FASTA scores: opt: 1226, E(): 7.6e-68, (40.7% identity in 543 aa overlap); Q9A7C3|CC1801 putative 4-coumarate--CoA ligase from Caulobacter crescentus (561 aa), FASTA scores: opt: 979, E(): 1.2e-52, (34.05% identity in 531 aa overlap); O28502|AF1772 long-chain-fatty-acid--CoA ligase (FADD-7) from Archaeoglobus fulgidus (569 aa), FASTA scores: opt: 560, E(): 6.9e-27, (29.3% identity in 543 aa overlap); Q9A8N2|CC1321 long-chain-fatty-acid--CoA ligase from Caulobacter crescentus (583 aa), FASTA scores: opt: 544, E(): 6.7e-26, (27.2% identity in 518 aa overlap); P29212|LCFA_ECOLI|FADD|OLDD|B1805 long-chain-fatty-acid--CoA ligase from Escherichia coli strain K12 (561 aa), FASTA scores: opt: 460, E(): 4e-22, (26.3% identity in 567 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Note that upstream MTV023.20c|Rv3513c|fadD18 is identical to C-terminal part of FADD19|Rv3515c|MTV023.22c (probably result of partial gene duplication). P9WQ51 No lipid metabolism Q7D5D8 P9WQ51 GO:0008152,GO:0016874 6.2.1.- P9WQ51 NC_000962.3 Mycobrowser_v5 CDS 3952544 3953335 . + 0 Rv3516 echA19 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Possible enoyl-CoA hydratase EchA19 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv3516, (MTV023.23), len: 263 aa. Possible echA19, enoyl-CoA hydratase, similar to other e.g. Q9ZHG2|ECHA1 from Rhodococcus fascians (275 aa) FASTA scores: opt: 613, E(): 6.4e-32, (45.15% identity in 259 aa overlap); P76082|PAAF_ECOLI|B1393 from Escherichia coli strain K12 (255 aa), FASTA scores: opt: 523, E(): 3.3e-26, (33.6% identity in 256 aa overlap); Q9I393|PA1629 from Pseudomonas aeruginosa (261 aa), FASTA scores: opt: 475, E(): 3.8e-23, (36.85% identity in 247 aa overlap); etc. Also similar to many carnitine racemases eg BAB52369|MLL6015 from Rhizobium loti (Mesorhizobium loti) (257 aa), FASTA scores: opt: 546, E(): 1.1e-27, (36.65% identity in 251 aa overlap). Similar to several putative enoyl-CoA hydratases from Mycobacterium tuberculosis, e.g. P96404|ECHA1|Rv0222|MTCY08D5.17 (262 aa), FASTA scores: opt: 630, E(): 5.1e-33, (44.5% identity in 254 aa overlap); and O53783|ECHA5|Rv0675|MTV040.03 (263 aa) FASTA scores: opt: 499, E(): 1.1e-24, (40.5% identity in 252 aa overlap). Could belong to the enoyl-CoA hydratase/isomerase family. O53561 No lipid metabolism O53561 O53561 GO:0008152,GO:0004300 4.2.1.17 O53561 NC_000962.3 Mycobrowser_v5 CDS 3953431 3954270 . + 0 Rv3517 Rv3517 Function unknown Conserved hypothetical protein Rv3517, (MTV023.24), len: 279 aa. Hypothetical protein, similar to several hypothetical mycobacterial proteins e.g. P71763|Rv1482c|MTCY277.03c from Mycobacterium tuberculosis strain H37Rv (339 aa) (alias AAK45794|MT1529 from Mycobacterium tuberculosis strain CDC1551 (292 aa) but longer) FASTA scores: opt: 1040, E(): 3.7e-60, (59.0% identity in 273 aa overlap); O07396|MAV346 from Mycobacterium avium (346 aa) FASTA scores: opt: 1018, E(): 1e-58, (57.2% identity in 278 aa overlap); O53421|Rv1073|MTV017.26 from Mycobacterium tuberculosis strain H37Rv (283 aa), FASTA scores: opt: 903, E(): 2.4e-51, (48.0% identity in 277 aa overlap); Q50134|U650AG|MLCB57.67c from Mycobacterium leprae (75 aa) FASTA scores: opt: 158, E(): 0.0015, (41.8% identity in 55 aa overlap); etc. O53562 No conserved hypotheticals O53562 O53562 NC_000962.3 Mycobrowser_v5 CDS 3954325 3955521 . - 0 Rv3518c cyp142 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Probable cytochrome P450 monooxygenase 142 Cyp142 Rv3518c, (MTV023.25c), len: 398 aa. Probable cyp142, cytochrome P450 monoxygenase, member of Cytochrome P450 family and similar to many e.g. Q9L465|CYP162A1|NIKQ from Streptomyces tendae (396 aa) FASTA scores: opt: 798, E(): 2e-43, (36.7% identity in 403 aa overlap); P33271|CPXK_SACER|CYP107B1 from Saccharopolyspora erythraea (Streptomyces erythraeus) (405 aa), FASTA scores: opt: 725, E(): 9.1e-39, (37.1% identity in 407 aa overlap); Q9X8Q3|CYP107P1|SCH10.14c from Streptomyces coelicolor (411 aa), FASTA scores: opt: 691, E(): 1.3e-36, (37.2% identity in 317 aa overlap); etc. Also similar to Q50696|C124_MYCTU|CYP124|Rv2266|MT2328|MTCY339.44c from Mycobacterium tuberculosis strain H37Rv (428 aa) FASTA scores: opt: 692, E(): 1.2e-36, (36.8% identity in 402 aa overlap). Equivalent to AAK47979 from Mycobacterium tuberculosis strain CDC1551 (372 aa) but longer 26 aa. Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. Belongs to the cytochrome P450 family. P9WPL5 No intermediary metabolism and respiration 2XKR O53563 P9WPL5 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPL5 NC_000962.3 Mycobrowser_v5 CDS 3956325 3957368 . - 0 Rv3520c Rv3520c Function unknown; probably involved in cellular metabolism. Possible coenzyme F420-dependent oxidoreductase Rv3520c, (MTV023.27c), len: 347 aa. Possible coenzyme F420-dependent oxidoreductase, equivalent to Q9CCV8|ML0348 possible coenzyme F420-dependent oxidoreductase from Mycobacterium leprae (350 aa), FASTA scores: opt: 2029, E(): 9.1e-120, (86.85% identity in 342 aa overlap). Similar to many coenzyme F420-dependent enzymes (and other proteins) e.g. Q9AD98|SCI52.11c putative ATP/GTP-binding protein from Streptomyces coelicolor (351 aa), FASTA scores: opt: 859, E(): 1.6e-46, (41.9% identity in 346 aa overlap); Q9X7Y1|SC6A5.35 putative oxidoreductase from Streptomyces coelicolor (341 aa), FASTA scores: opt: 800, E(): 7.9e-43, (38.95% identity in 339 aa overlap); Q9ZA30|GRA-ORF29 putative FMN-dependent monooxygenase from Streptomyces violaceoruber (343 aa), FASTA scores: opt: 354, E(): 6.7e-15, (34.2% identity in 336 aa overlap); Q49598|mer coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin reductase from Methanopyrus kandleri (349 aa), FASTA scores: opt: 283, E(): 1.9e-10, (26.75% identity in 329 aa overlap); Q58929|mer|MJ1534 F420-dependent methylenetetrahydromethanopterin reductase from Methanococcus jannaschii (331 aa), FASTA scores: opt: 227, E(): 5.8e-07, (26.35% identity in 334 aa overlap); O27784|MTH1752 coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase from Methanobacterium thermoautotrophicum (321 aa), FASTA scores: opt: 207, E(): 1e-05, (27.4% identity in 336 aa overlap); etc. Also similar to Q11030|YD60_MYCTU|Rv1360|MT1405|MTCY02B10.24 hypothetical 37.3 KDA protein from Mycobacterium tuberculosis (340 aa), FASTA scores: opt: 313, E(): 2.5e-12, (28.0% identity in 311 aa overlap). O53565 No intermediary metabolism and respiration O53565 O53565 GO:0016491,GO:0055114 1.-.-.- O53565 NC_000962.3 Mycobrowser_v5 CDS 3967038 3967820 . - 0 Rv3530c Rv3530c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv3530c, (MTCY03C7.26), len: 260 aa. Possible oxidoreductase, similar to various oxidoreductases and hypothetical proteins e.g. BAB53258|Q987E5|MLL7083 probable oxidoreductase from Rhizobium loti (Mesorhizobium loti) (258 aa), FASTA scores: opt: 405, E(): 5.3e-18, (33.45% identity in 263 aa overlap); Q9VNF3|CG12171 hypothetical protein from Drosophila melanogaster (Fruit fly) (257 aa), FASTA scores: opt: 404, E(): 6.1e-18, (32.8% identity in 256 aa overlap); Q9A3X5|CC3076 oxidoreductase (short-chain dehydrogenase/reductase family) from Caulobacter crescentus (254 aa), FASTA scores: opt: 400, E(): 1.1e-17, (31.0% identity in 255 aa overlap); BAB50080|MLR3115 dehydrogenase from Rhizobium loti (Mesorhizobium loti) (259 aa), FASTA scores: opt: 393, E(): 3e-17, (31.9% identity in 254 aa overlap); Q9F5J1|SIM-NJ1|SIMD2 putative 3-keto-acyl-reductase from Streptomyces antibioticus (273 aa), FASTA scores: opt: 388, E(): 6.3e-17, (31.6% identity in 250 aa overlap); etc. I6Y3S9 No intermediary metabolism and respiration P71871 I6Y3S9 GO:0055114,GO:0016491 1.-.-.- I6Y3S9 NC_000962.3 Mycobrowser_v5 CDS 3961800 3962324 . - 0 Rv3525c Rv3525c Function unknown Possible siderophore-binding protein Rv3525c, (MTCY3C7.31), len: 174 aa. Possible siderophore-binding protein, similar to ferripyochelin binding proteins (and related) e.g. Q9RSN5|DR2089 ferripyochelin-binding protein from Deinococcus radiodurans (240 aa), FASTA scores: opt: 472, E(): 3.3e-21, (46.9% identity in 162 aa overlap); O59257|PH1591 long hypothetical ferripyochelin binding protein from Pyrococcus horikoshii (173 aa), FASTA scores: opt: 431, E(): 6.7e-19, (40.0% identity in 170 aa overlap); Q9V158|FBP|PAB0393 ferripyochelin binding protein from Pyrococcus abyssi (173 aa), FASTA scores: opt: 429, E(): 8.9e-19, (39.4% identity in 170 aa overlap); BAB47820|MLR0180 ferripyochelin binding protein-like from Rhizobium loti (Mesorhizobium loti) (175 aa), FASTA scores: opt: 415, E(): 6.1e-18, (42.55% identity in 141 aa overlap); etc. I6YCB9 No intermediary metabolism and respiration I6YCB9 GO:0016740 I6YCB9 NC_000962.3 Mycobrowser_v5 CDS 3967817 3968944 . - 0 Rv3531c Rv3531c Unknown Hypothetical protein Rv3531c, (MTCY03C7.25), len: 375 aa. Hypothetical unknown protein. I6XHH2 No conserved hypotheticals I6XHH2 I6XHH2 NC_000962.3 Mycobrowser_v5 CDS 3979499 3980659 . - 0 Rv3540c ltp2 Function unknown, supposed involvement in lipid metabolism. Probable lipid transfer protein or keto acyl-CoA thiolase Ltp2 Rv3540c, (MTCY03C7.16), len: 386 aa. Probable ltp2, lipid-transfer protein or keto acyl-CoA thiolase, similar to several e.g. Q9X4X2|DITF DITF protein (hypothetical protein, similar to non-specific lipid-transfer protein and 3-ketoacyl-CoA thiolase) from Pseudomonas abietaniphila (397 aa), FASTA scores: opt: 665, E(): 5.3e-34, (33.4% identity in 392 aa overlap); O30255|AF2416 3-ketoacyl-CoA thiolase (ACAB-12) from Archaeoglobus fulgidus (384 aa), FASTA scores: opt: 496, E(): 1.6e-23, (30.35% identity in 389 aa overlap); O28978|AF1291 3-ketoacyl-CoA thiolase (ACAB-11) from Archaeoglobus fulgidus (392 aa), FASTA scores: opt: 494, E(): 2.2e-23, (30.6% identity in 379 aa overlap); O26884|MTH793 lipid-transfer protein (sterol or nonspecific) from Methanobacterium thermoautotrophicum (383 aa), FASTA scores: opt: 487, E(): 5.9e-23, (30.4% identity in 388 aa overlap); etc. I6Y3T7 No lipid metabolism I6Y3T7 GO:0008152,GO:0003988 2.3.1.16 I6Y3T7 NC_000962.3 Mycobrowser_v5 CDS 4028968 4029762 . - 0 Rv3587c Rv3587c Function unknown Probable conserved membrane protein Rv3587c, (MTV024.05c), len: 264 aa. Probable conserved membrane protein, equivalent to Q9CBJ2|ML1918 hypothetical membrane protein from Mycobacterium leprae (263 aa), FASTA scores: opt: 1438, E(): 2.4e-57, (77.55% identity in 267 aa overlap). Contains hydrophobic stretch in N-terminus; possible signal sequence. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). O53572 No cell wall and cell processes O53572 O53572 NC_000962.3 Mycobrowser_v5 CDS 3973589 3974500 . - 0 Rv3535c hsaG Supposed involvement in one, or several, catabolic pathways [catalytic activity: acetaldehyde + CoA + NAD(+) = acetyl-CoA + NADH]. Predicted to be involved in lipid catabolism. Probable acetaldehyde dehydrogenase (acetaldehyde dehydrogenase [acetylating]) Rv3535c, (MTCY03C7.21), len: 303 aa. Probable hsaG, acetaldehyde dehydrogenase, highly similar to many e.g. BAB62056|TDNI from Pseudomonas putida (302 aa), FASTA scores: opt: 1159, E(): 1.5e-62, (60.45% identity in 301 aa overlap); Q9ZI57|NAHO from Pseudomonas stutzeri (Pseudomonas perfectomarina) (307 aa) FASTA scores: opt: 1151, E(): 4.6e-62, (59.55% identity in 299 aa overlap); Q9F9I4|CDOI from Comamonas sp. JS765 (302 aa) FASTA scores: opt: 1136, E(): 3.6e-61, (60.15% identity in 301 aa overlap); Q51962|NAHO from Pseudomonas putida (307 aa), FASTA scores: opt: 1133, E(): 5.6e-61, (58.55% identity in 299 aa overlap) (see citation below); P77580|MHPF_ECOLI|MHPF|MHPE|B0351 from Escherichia coli strain K12 (316 aa), FASTA scores: opt: 1040, E(): 2.2e-55, (56.85% identity in 306 aa overlap); etc. P9WQH3 No intermediary metabolism and respiration P71866 P9WQH3 GO:0008774,GO:0019439,GO:0006520,GO:0005737,GO:0055114,GO:0051287 1.2.1.10 P9WQH3 NC_000962.3 Mycobrowser_v5 CDS 4197236 4197595 . + 0 Rv3748 Rv3748 Function unknown Conserved hypothetical protein Rv3748, (MTV025.096), len: 119 aa. Hypothetical protein, highly similar to upstream ORF O69714|Rv3747|MTV025.095 conserved hypothetical protein (127 aa), FASTA scores: opt: 496, E(): 2.5e-28, (64.4% identity in 118 aa overlap). O69715 No conserved hypotheticals O69715 NC_000962.3 Mycobrowser_v5 CDS 3989153 3989896 . + 0 Rv3550 echA20 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Probable enoyl-CoA hydratase EchA20 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv3550, (MTCY03C7.06c), len: 247 aa. Probable echA20, enoyl-CoA hydratase, similar to others e.g. Q9A7B0|CC1814 from Caulobacter crescentus (275 aa), FASTA scores: opt: 488, E(): 3.5e-24, (36.4% identity in 239 aa overlap); O84978|PHAA from Pseudomonas putida (293 aa), FASTA scores: opt: 383, E(): 2e-17, (33.85% identity in 254 aa overlap); BAB48479|Q98LI4|MLL1009 from Rhizobium loti (Mesorhizobium loti) (258 aa), FASTA scores: opt: 378, E(): 3.8e-17, (21.45% identity in 231 aa overlap); etc. Could belong to the enoyl-CoA hydratase/isomerase family. I6Y3U6 No lipid metabolism P71851 I6Y3U6 GO:0016853,GO:0008152,GO:0004300 4.2.1.17 I6Y3U6 NC_000962.3 Mycobrowser_v5 CDS 4199247 4199705 . - 0 Rv3752c Rv3752c Function unknown; probably involved in deamination of specific substrate. Possible cytidine/deoxycytidylate deaminase Rv3752c, (MTV025.100c), len: 152 aa. Probable cytidine/deoxycytidylate deaminase, equivalent to Q9CB32|ML2474 possible cytidine/deoxycytidylate deaminase from Mycobacterium leprae (171 aa), FASTA scores: opt: 890, E(): 1.6e-50, (88.1% identity in 151 aa overlap). Also highly similar to other deaminases and hypothetical proteins e.g. Q9AK79|2SCD60.04c putative deaminase from Streptomyces coelicolor (143 aa), FASTA scores: opt: 559, E(): 2.9e-29, (66.45% identity in 146 aa overlap); Q9F9W7 cytosine deaminase from Bifidobacterium longum (143 aa) FASTA scores: opt: 512, E(): 3.1e-26, (54.85% identity in 144 aa overlap); P21335|YAAJ_BACSU hypothetical 17.8 KDA protein from Bacillus subtilis (161 aa), FASTA scores: opt: 425, E(): 1.4e-20, (47.7% identity in 151 aa overlap); AAK74212|SP0020 cytidine/deoxycytidylate deaminase family protein from Streptococcus pneumoniae (155 aa), FASTA scores: opt: 401, E(): 4.7e-19, (46.25% identity in 147 aa overlap); P30134|YFHC_ECOLI|B2559 hypothetical 20.0 KDA protein from Escherichia coli strain K12 (178 aa), FASTA scores: opt: 397, E(): 9.5e-19, (47.0% identity in 149 aa overlap); etc. Contains PS00903 Cytidine and deoxycytidylate deaminases zinc-binding region signature. Belongs to the cytidine and deoxycytidylate deaminases family. O69719 No intermediary metabolism and respiration O69719 O69719 GO:0008270,GO:0016787 3.5.4.- O69719 NC_000962.3 Mycobrowser_v5 CDS 3975369 3977060 . + 0 Rv3537 kstD Function unknown; probably involved in cellular metabolism. Predicted to be involved in lipid catabolism. Probable dehydrogenase Rv3537, (MTCY03C7.19c), len: 563 aa. Probable kstD, dehydrogenase, similar to many dehydrogenases or hypothetical proteins e.g. Q9I1M6|PA2243 hypothetical protein from Pseudomonas aeruginosa (577 aa), FASTA scores: opt: 984, E(): 1.2e-48, (34.75% identity in 573 aa overlap); Q06401|3O1D_COMTE 3-oxosteroid 1-dehydrogenase from Comamonas testosteroni (Pseudomonas testosteroni) (573 aa), FASTA scores: opt: 955, E(): 5.5e-47, (33.05% identity in 590 aa overlap); Q9RA02|KSTD1 3-ketosteroid dehydrogenase from Rhodococcus erythropolis (510 aa), FASTA scores: opt: 631, E(): 1.4e-28, (39.15% identity in 557 aa overlap); P77815|KSDD 3-ketosteroid-1-dehydrogenase from Nocardioides simplex (Arthrobacter simplex) (515 aa), FASTA scores: opt: 469, E(): 2.4e-19, (35.45% identity in 564 aa overlap); etc. P71864 No intermediary metabolism and respiration P71864 P71864 GO:0055114,GO:0016491,GO:0009055 1.-.-.- P71864 NC_000962.3 Mycobrowser_v5 CDS 3978059 3979498 . + 0 Rv3539 PPE63 Function unknown PPE family protein PPE63 Rv3539, (MTCY03C7.17c), len: 479 aa. PPE63, Member of the Mycobacterium tuberculosis PPE protein family, similar to many e.g. O53949|Rv1800|MTV049.22 (655 aa), FASTA scores: opt: 914, E(): 7.3e-47, (37.55% identity in 490 aa overlap); etc. P9WHX5 No PE/PPE Q7D5B9 P9WHX5 P9WHX5 NC_000962.3 Mycobrowser_v5 CDS 3985557 3986732 . + 0 Rv3546 fadA5 Function unknown, but involved in lipid degradation [catalytic activity: 2 acetyl-CoA = CoA + acetoacetyl-CoA]. Probable acetyl-CoA acetyltransferase FadA5 (acetoacetyl-CoA thiolase) Rv3546, (MTCY03C7.10c), len: 391 aa. Probable fadA5, acetyl-CoA acetyltransferase, similar to many e.g. Q9AA29|CC0779 from Caulobacter crescentus (390 aa), FASTA scores: opt: 999, E(): 7.1e-54, (43.5% identity in 400 aa overlap); Q9K783|BH3487 from Bacillus halodurans (393 aa), FASTA scores: opt: 843, E(): 2.6e-44, (37.45% identity in 398 aa overlap); Q9RRK9|DR2480 from Deinococcus radiodurans (399 aa), FASTA scores: opt: 826, E(): 2.8e-43, (38.15% identity in 396 aa overlap); P45369|THIL_CHRVI|PHBA from Chromatium vinosum (394 aa) FASTA scores: opt: 790, E(): 4.5e-41, (39.4% identity in 401 aa overlap); etc. Contains PS00737 Thiolases signature 2. Belongs to the thiolase family. I6XHI4 No lipid metabolism P71855 I6XHI4 GO:0008152,GO:0003985 2.3.1.9 I6XHI4 NC_000962.3 Mycobrowser_v5 CDS 3986844 3987299 . + 0 Rv3547 ddn Function unknown. Converts bicyclic nitroimidazole drug candidate pa-824 to three metabolites, generating NO. Deazaflavin-dependent nitroreductase Ddn Rv3547, (MTCY03C7.09c), len: 151 aa. Ddn, deazaflavin-dependent nitroreducatse (See Singh et al., 2008). Similar to hypothetical proteins e.g. O85698|3SCF60.07 from Streptomyces lividans and Streptomyces coelicolor (149 aa), FASTA scores: opt: 353, E(): 6.3e-17, (42.55% identity in 134 aa overlap); Q9WX21|SCE68.11 from Streptomyces coelicolor (305 aa) FASTA scores: opt: 290, E(): 2.1e-12, (38.5% identity in 122 aa overlap) (similarity in N-terminus for this protein); BAB52932|Q988L5|MLL6688 from Rhizobium loti (Mesorhizobium loti) (148 aa), FASTA scores: opt: 105, E(): 3, (26.75% identity in 86 aa overlap). Also similar to mycobacterial hypothetical proteins e.g. Q9ZH81 from Mycobacterium paratuberculosis (144 aa), FASTA scores: opt: 366, E(): 8.2e-18, (43.9% identity in 123 aa overlap); and Q10772|YF58_MYCTU|Rv1558|MT1609|MTCY48.07c from Mycobacterium tuberculosis (148 aa), FASTA scores: opt: 330, E(): 2.2e-15, (39.75% identity in 151 aa overlap); etc. Predicted to be an outer membrane protein (See Song et al., 2008). P9WP15 No intermediary metabolism and respiration 3R5L,3R5P,3R5R,3R5W P71854 P9WP15 P9WP15 NC_000962.3 Mycobrowser_v5 CDS 3987382 3988296 . - 0 Rv3548c Rv3548c Function unknown; supposed involvement in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv3548c, (MTCY03C7.08), len: 304 aa. Probable short-chain dehydrogenase/reductase, highly similar to various dehydrogenases/reductases (generally belonging to the SDR family) e.g. Q9I4V1|PA1023 from Pseudomonas aeruginosa (305 aa), FASTA scores: opt: 446, E(): 1.7e-17, (43.75% identity in 256 aa overlap); Q9A6K0|CC2093 from Caulobacter crescentus (301 aa) FASTA scores: opt: 437, E(): 5.3e-17, (42.8% identity in 257 aa overlap); Q9HYH8|PA3427 from Pseudomonas aeruginosa (303 aa), FASTA scores: opt: 399, E(): 6.5e-15, (45.5% identity in 257 aa overlap); Q9VXJ0|CG3415 from Drosophila melanogaster (Fruit fly) (598 aa), FASTA scores: opt: 402, E(): 7.5e-15, (40.7% identity in 285 aa overlap); etc. Also highly similar to O53547|Rv3502c|MTV023.09c putative short-chain type dehydrogenase/reductase from (317 aa) FASTA scores: opt: 739, E(): 1.6e-33, (45.15% identity in 310 aa overlap); and other proteins from Mycobacterium tuberculosis. Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. P71853 No intermediary metabolism and respiration P71853 P71853 GO:0055114,GO:0016491 1.-.-.- P71853 NC_000962.3 Mycobrowser_v5 CDS 4029871 4030494 . - 0 Rv3588c canB Catalyzes reversible dehydration of CO2 to form bicarbonate Beta-carbonic anhydrase CanB Rv3588c, (MTV024.06c), len: 207 aa. CanB, Beta-carbonic anhydrase, proven biochemically (See Suarez Covarrubias et al. 2005) similar to others e.g. Q9CBJ1|ML1919 putative carbonic anhydrase from Mycobacterium leprae (213 aa), FASTA scores: opt: 1160, E(): 3.1e-66, (84.55% identity in 207 aa overlap). Also similar to many e.g. Q9X903|SCH35.03 from Streptomyces coelicolor (207 aa), FASTA scores: opt: 689, E(): 1.6e-36, (53.85% identity in 195 aa overlap); Q9RS89|DR2238 from Deinococcus radiodurans (264 aa), FASTA scores: opt: 451, E(): 2e-21, (39.7% identity in 189 aa overlap); Q39589|beta-CA1 from Chlamydomonas reinhardtii (267 aa) FASTA scores: opt: 419, E(): 2.1e-19, (36.55% identity in 197 aa overlap); etc. Contains PS00704 and PS00705 Prokaryotic-type carbonic anhydrases signature 1 and 2. Belongs to the plant and prokaryotic carbonic anhydrase family. P9WPJ9 No intermediary metabolism and respiration 1YM3,2A5V O53573 P9WPJ9 GO:0004089,GO:0008270,GO:0015976 4.2.1.1 P9WPJ9 NC_000962.3 Mycobrowser_v5 CDS 3988319 3989098 . - 0 Rv3549c Rv3549c Function unknown; supposed involvement in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv3549c, (MTCY03C7.07), len: 259 aa. Probable short-chain dehydrogenase/reductase, similar to various dehydrogenases/reductases (generally belong to the SDR family) e.g. Q9UKU3 from Homo sapiens (Human) (270 aa), FASTA scores: opt: 451, E(): 4.8e-21, (38.05% identity in 247 aa overlap); Q9S274|SCI28.09c from Streptomyces coelicolor (234 aa), FASTA scores: opt: 439, E(): 2.4e-20, (36.8% identity in 231 aa overlap); Q9PFI6|XF0671 from Xylella fastidiosa (247 aa), FASTA scores: opt: 437, E(): 3.4e-20, (37.7% identity in 252 aa overlap); etc. Also highly similar to O33308|FABG5|Rv2766c|MTV002.31c alcohol dehydrogenase (SDR family) from Mycobacterium tuberculosis (260 aa), FASTA scores: opt: 504, E(): 2.3e-24, (38.5% identity in 244 aa overlap). Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. I6YCE1 No intermediary metabolism and respiration P71852 I6YCE1 GO:0055114,GO:0016491 1.-.-.- I6YCE1 NC_000962.3 Mycobrowser_v5 CDS 3989896 3990774 . + 0 Rv3551 Rv3551 Function unknown. Probable subunit of a CoA-transferase composed of Rv3551|MTCY03C7.05c and Rv3552|MTCY03C7.03c. Possible CoA-transferase (alpha subunit) Rv3551, (MTCY03C7.05c), len: 292 aa. Possible CoA-transferase, alpha subunit, similar in part to other CoA-transferases e.g. Q59111|GCTA_ACIFE|GCTA glutaconate CoA-transferase subunit A (GCT large subunit) from Acidaminococcus fermentans (319 aa) FASTA scores: opt: 247, E(): 6.3e-09, (27.35% identity in 307 aa overlap); Q9XD83|PCAI from Streptomyces sp. 2065 (251 aa), FASTA scores: opt: 222, E(): 2.3e-07, (27.55% identity in 243 aa overlap); BAB50895|MLL4183 from Rhizobium loti (Mesorhizobium loti) (285 aa), FASTA scores: opt: 206, E(): 2.8e-06, (27.4% identity in 281 aa overlap); etc. Also some similarity with O06167|SCOA_MYCTU|RVv504c|MT2579|MTCY07A7.10c probable succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A from Mycobacterium tuberculosis (248 aa), FASTA scores: opt: 210, E(): 1.4e-06, (25.5% identity in 247 aa overlap). Belongs to the glutaconate CoA-transferase subunit A family. Note that this putative protein may combine with the putative protein encoded by the downstream ORF Rv3552 to form a CoA-transferase that comprises two subunits. P9WPW1 No intermediary metabolism and respiration P71850 P9WPW1 GO:0008152,GO:0008410 2.8.3.- P9WPW1 NC_000962.3 Mycobrowser_v5 CDS 3990771 3991523 . + 0 Rv3552 Rv3552 Function unknown. Probable subunit of a CoA-transferase composed of Rv3551|MTCY03C7.05c and Rv3552|MTCY03C7.03c. Possible CoA-transferase (beta subunit) Rv3552, (MTCY03C7.03c), len: 250 aa. Possible CoA-transferase, beta subunit, similar in part to other CoA-transferases e.g. Q9I6R1|PA0227 from Pseudomonas aeruginosa (260 aa), FASTA scores: opt: 233, E(): 8.6e-08, (24.8% identity in 238 aa overlap); BAB50894|MLL4181 from Rhizobium loti (Mesorhizobium loti) (264 aa), FASTA scores: opt: 210, E(): 2.6e-06, (24.15% identity in 203 aa overlap); and AAK41345|Q97Z51|GCTB from Sulfolobus solfataricus (245 aa), FASTA scores: opt: 122, E(): 1.1, (25.5% identity in 243 aa overlap). Possibly belongs to the glutaconate CoA-transferase subunit B family. Note that this putative protein may combine with the putative protein encoded by the upstream ORF Rv3551 to form a CoA-transferase that comprises two subunits. P9WPV9 No intermediary metabolism and respiration P9WPV9 GO:0016740 2.8.3.- P9WPV9 NC_000962.3 Mycobrowser_v5 CDS 3997029 3997631 . - 0 Rv3557c Rv3557c Involved in transcriptional mechanism. Transcriptional regulatory protein (probably TetR-family) Rv3557c, (MTCY06G11.04c), len: 200 aa. Transcriptional regulator, TetR family, similar to other e.g. Q9RRV9|DR2376 from Deinococcus radiodurans (197 aa) FASTA scores: opt: 326, E(): 2.3e-14, (31.2% identity in 189 aa overlap); Q9HZW2|PA2885 from Pseudomonas aeruginosa (198 aa), FASTA scores: opt: 308, E(): 3.5e-13, (31.55% identity in 187 aa overlap); Q9RFR4 from Pseudomonas fluorescens (207 aa), FASTA scores: opt: 291, E(): 4.7e-12, (29.75% identity in 195 aa overlap); Q9K8P5|BH2958 from Bacillus halodurans (215 aa), FASTA scores: opt: 271, E(): 9.9e-11, (23.95% identity in 192 aa overlap); etc. Also similar to proteins from Mycobacterium tuberculosis e.g. O53641|Rv0158|MTV032.01 (214 aa), FASTA scores: opt: 232, E(): 3.5e-08, (25.5% identity in 192 aa overlap); and O06169|Rv2506|MTCY07A7.12 (215 aa), FASTA scores: opt: 215, E(): 4.5e-07, (35.15% identity in 148 aa overlap); etc. Seems to belong to the TetR/AcrR family of transcriptional regulators. P9WMB9 No regulatory proteins P96839 P9WMB9 GO:0006355,GO:0016566,GO:0006350,GO:0003700,GO:0016481 P9WMB9 NC_000962.3 Mycobrowser_v5 CDS 3999647 4000435 . - 0 Rv3559c Rv3559c Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv3559c, (MTCY06G11.06c), len: 262 aa. Probable oxidoreductase, similar to various oxidoreductases e.g. Q9F5J1|SIM-NJ1|SIMD2 putative 3-keto-acyl-reductase (SDR family) from Streptomyces antibioticus (273 aa), FASTA scores: opt: 510, E(): 2.8e-24, (40.15% identity in 249 aa overlap);Q9L2C9|SC7A8.29 putative dehydrogenase from Streptomyces coelicolor (255 aa), FASTA scores: opt: 500, E(): 1.1e-23, (41.4% identity in 239 aa overlap); Q9HQ41|FABG|VNG1341G 3-oxoacyl-[acyl-carrier-protein] reductase from Halobacterium sp. strain NRC-1 (255 aa) FASTA scores: opt: 500, E(): 1.1e-23, (40.0% identity in 250 aa overlap); etc. Also similar to oxidoreductases from Mycobacterium tuberculosis eg Q11020|YD50_MYCTU|FABG2|Rv1350|MT1393|MTCY02B10.14 putative oxidoreductase (247 aa), FASTA scores: opt: 497, E(): 1.6e-23, (39.2% identity in 245 aa overlap). I6YCF0 No intermediary metabolism and respiration P96841 I6YCF0 GO:0055114,GO:0016491 1.-.-.- I6YCF0 NC_000962.3 Mycobrowser_v5 CDS 4003161 4004294 . + 0 Rv3562 fadE31 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE31 Rv3562, (MTCY06G11.09), len: 377 aa. Probable fadE31, acyl-CoA dehydrogenase, similar to many e.g. Q9RJX2|SCF37.29c from Streptomyces coelicolor (393 aa), FASTA scores: opt: 657, E(): 1.7e-34, (36.45% identity in 351 aa overlap); Q9A5G9|CC2478 from Caulobacter crescentus (407 aa), FASTA scores: opt: 653, E(): 3.2e-34, (33.95% identity in 392 aa overlap); Q9EX72|MLHC from Rhodococcus erythropolis (324 aa) FASTA scores: opt: 631, E(): 6.5e-33, (36.95% identity in 330 aa overlap); P45867|ACDA_BACSU|ACD from Bacillus subtilis (379 aa), FASTA scores: opt: 347, E(): 1e-15, (28.6% identity in 385 aa overlap); etc. Also similar to other from Mycobacterium tuberculosis e.g. P96842|FADE30|Rv3560c|MTCY06G11.07c (385 aa), FASTA scores: opt: 843, E(): 2.3e-46, (38.95% identity in 380 aa overlap). Could belong to the acyl-CoA dehydrogenases family. I6YGH7 No lipid metabolism P96844 I6YGH7 GO:0003995,GO:0055114,GO:0050660 1.3.99.- I6YGH7 NC_000962.3 Mycobrowser_v5 CDS 4011086 4012270 . - 0 Rv3570c hsaA Function unknown; probably involved in cellular metabolism. Predicted to be involved in lipid catabolism. Possible oxidoreductase. Possible 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione hydroxylase. Rv3570c, (MTCY06G11.17c), len: 394 aa. Possible hsaA, oxidoreductase, most similar to hydroxylases and oxygenases (and also some similarity to acyl-CoA dehydrogenases) e.g. O69349 hydroxylase from Rhodococcus erythropolis (393 aa), FASTA scores: opt: 958, E(): 1.1e-53, (39.95% identity in 383 aa overlap); P26698|PIGM_RHOSO pigment protein from Rhodococcus sp. strain ATCC 21145 (387 aa), FASTA scores: opt: 665, E(): 5.4e-35, (32.2% identity in 382 aa overlap); Q9ZGA9|LANZ5 oxygenase homolog from Streptomyces cyanogenus (397 aa) FASTA scores: opt: 588, E(): 4.5e-30, (30.55% identity in 386 aa overlap); Q9F0J3|NCNH hydroxylase from Streptomyces arenae (405 aa), FASTA scores: opt: 580, E(): 1.5e-29, (31.25% identity in 336 aa overlap); O69789|BPFA indole dioxygenase from Rhodococcus opacus (399 aa), FASTA scores: opt: 558, E(): 3.7e-28, (31.8% identity in 387 aa overlap); etc. P9WJA1 No intermediary metabolism and respiration 3AFE,3AFF P96852 P9WJA1 GO:0003995,GO:0055114,GO:0050660 1.-.-.- P9WJA1 NC_000962.3 Mycobrowser_v5 CDS 4050601 4052883 . - 0 Rv3610c ftsH Thought to act as an ATP-dependent zinc metallopeptidase, with ATPase and proteolytic activities. Probably has a regulatory role in stress response and specific proteins secretion for adaptation to host environment. Membrane-bound protease FtsH (cell division protein) Rv3610c, (MT3714, MTCY07H7B.12), len: 760 aa. FtsH, membrane-bound protease (cell division protein) (see citations below), equivalent to Q9CD58|FTSH_MYCLE|ML0222 (alias O69532|FTSH) cell division protein FTSH homolog from Mycobacterium leprae (787 aa), FASTA scores: opt: 4388, E(): 9.6e-205, (87.2% identity in 790 aa overlap). Also highly similar to many FTSH proteins e.g. O52395|FTSH from Mycobacterium smegmatis (769 aa), FASTA scores: opt: 3976, E(): 7.6e-185, (82.4% identity in 761 aa overlap); Q9X8I4|SCE9.11c from Streptomyces coelicolor (668 aa), FASTA scores: opt: 2417, E(): 1.4e-109, (57.2% identity in 668 aa overlap); P72991|FTH4_SYNY3|SLR1604 from Synechocystis sp. strain PCC 6803 (616 aa), FASTA scores: opt: 1926, E(): 7.2e-86, (49.35% identity in 612 aa overlap); P28691|FTSH_ECOLI|HFLB|MRSC|TOLZ|B3178 from Escherichia coli strain K12 (644 aa), FASTA scores: opt: 1859, E(): 1.3e-82, (48.95% identity in 605 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00674 AAA-protein family signature. Belongs to the AAA family of ATPases and peptidase family M41 (zinc metalloprotease). Cofactor: binds one zinc ion (potential). Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P9WQN3 No cell wall and cell processes P0C5C0 P9WQN3 GO:0007049,GO:0051301,GO:0016021,GO:0046872,GO:0004222,GO:0017111,GO:0005886,GO:0030163,GO:0006508,GO:0005524 3.4.24.- P9WQN3 NC_000962.3 Mycobrowser_v5 CDS 4007331 4008182 . - 0 Rv3566c nat Could have a role in acetylating, and hence inactivating, the antitubercular drug isoniazid [catalytic activity: acetyl-CoA + arylamine = CoA + N-acetylarylamine]. Arylamine N-acetyltransferase Nat (arylamine acetylase) Rv3566c, (MT3671, MTCY06G11.13c), len: 283 aa. Nat (alternate gene name: nhoA), arylamine N-acetyltransferase (see citations below), highly similar to O86309|NAT_MYCSM arylamine N-acetyltransferase from Mycobacterium smegmatis (see citation below) (275 aa), FASTA scores: opt: 1114, E(): 3e-66, (60.95% identity in 274 aa overlap). Also highly similar to others e.g. Q98D42|BAB51429|MLR4870 from Rhizobium loti (Mesorhizobium loti) (278 aa), FASTA scores: opt: 697, E(): 1.1e-38, (44.1% identity in 272 aa overlap); P77567|NHOA_ECOLI|B1463 from Escherichia coli strain K12 (281 aa), FASTA scores: opt: 537, E(): 4.4e-28, (38.85% identity in 273 aa overlap); Q00267|NHOA_SALTY from Salmonella typhimurium (281 aa), FASTA scores: opt: 507, E(): 4.3e-26, (34.8% identity in 273 aa overlap); etc. Belongs to the arylamine N-acetyltransferase family. Note that previously known as nhoA (332 aa) and that nucleotide 4007874 has been changed since first submission (G deleted). P9WJI5 No intermediary metabolism and respiration P0A5L8 P9WJI5 GO:0005737,GO:0008152,GO:0004060 2.3.1.5 INH P9WJI5 NC_000962.3 Mycobrowser_v5 CDS 4008719 4009282 . - 0 Rv3567c hsaB Function unknown; probably involved in cellular metabolism. Predicted to be involved in lipid catabolism. Possible oxidoreductase. Possible 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione hydroxylase. Rv3567c, (MTCY06G11.14c), len: 187 aa. Possible hsaB, oxidoreductase, similar to various oxidoreductases and hypothetical proteins e.g. O69360 ORF61 protein from Rhodococcus erythropolis (194 aa) FASTA scores: opt: 974, E(): 3e-59, (77.05% identity in 183 aa overlap); Q9JN75|MMYF putative oxidoreductase from Streptomyces coelicolor (174 aa), FASTA scores: opt: 451, E(): 1e-23, (43.65% identity in 158 aa overlap); P54990|NTAB_CHEHE|NMOB nitrilotriacetate monooxygenase component B from Chelatobacter heintzii (322 aa), FASTA scores: opt: 409, E(): 1.3e-20, (38.3% identity in 167 aa overlap)Chelatobacter heintzii; AAK62356 putative NADH:FMN oxidoreductase from Burkholderia sp. DBT1 (177 aa), FASTA scores: opt: 360, E(): 1.6e-17, (36.15% identity in 155 aa overlap). P9WND9 No intermediary metabolism and respiration P96849 P9WND9 GO:0055114,GO:0016491,GO:0010181 1.-.-.- P9WND9 NC_000962.3 Mycobrowser_v5 CDS 4009297 4010199 . - 0 Rv3568c hsaC Catalyzes the extradiol cleavage of 3,4-dihydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione (3,4-DHSA) 3,4-DHSA dioxygenase Rv3568c, (MTCY06G11.15c), len: 300 aa. HsaC, highly similar to e.g. Q9KWQ5|BPHC5 from Rhodococcus sp. RHA1 (300 aa), FASTA scores: opt: 1715, E(): 3.8e-103, (82.15% identity in 297 aa overlap); O50479|EDOB from Rhodococcus rhodochrous (300 aa) FASTA scores: opt: 1714, E(): 4.4e-103, (82.5% identity in 297 aa overlap); O69359|BPHC6 from Rhodococcus erythropolis (300 aa), FASTA scores: opt: 1647, E(): 9.1e-99, (78.25% identity in 299 aa overlap); Q9RBT2|BPHC1 from Pseudomonas sp. SY5 (301 aa) Pseudomonas sp. SY5 (298 aa) FASTA scores: opt: 767, E(): 3.9e-42, (42.8% identity in 299 aa overlap); P47228|BPHC_BURCE from Burkholderia cepacia (Pseudomonas cepacia) (297 aa), FASTA scores: opt: 670, E(): 6.8e-36, (40.55% identity in 296 aa overlap); etc. Contains PS00082 Extradiol ring-cleavage dioxygenases signature. Belongs to the extradiol ring-cleavage dioxygenase family. P9WNW7 No intermediary metabolism and respiration 2ZI8,2ZYQ P96850 P9WNW7 GO:0018583,GO:0008198,GO:0055114,GO:0019439 1.13.11.39 P9WNW7 NC_000962.3 Mycobrowser_v5 CDS 4016484 4017083 . + 0 Rv3574 kstR Involved in transcriptional mechanism. Predicted to control regulon involved in lipid metabolism Transcriptional regulatory protein KstR (probably TetR-family) Rv3574, (MTCY06G11.21), len: 199 aa. Probable kstR, transcriptional regulator TetR family, similar to others e.g. Q9KXK1|SCC53.10 from Streptomyces coelicolor (250 aa) FASTA scores: opt: 492, E(): 4.8e-25, (44.8% identity in 183 aa overlap); Q9RA03|KSTR from Rhodococcus erythropolis (208 aa), FASTA scores: opt: 294, E(): 3.1e-12, (28.9% identity in 187 aa overlap); BAB54261|MLR7895 from Rhizobium loti (Mesorhizobium loti) (193 aa), FASTA scores: opt: 166, E(): 0.00062, (32.05% identity in 78 aa overlap); P17446|BETI_ECOLI|B0313 from Escherichia coli strain K12 (195 aa), FASTA scores: opt: 142, E(): 0.0034, (25. 6% identity in 168 aa overlap); etc. Equivalent to AAK48038 from Mycobacterium tuberculosis strain CDC1551 (243 aa) but shorter 44 aa. Contains possible helix-turn-helix motif from aa 37-58 (+3.70 SD). Possibly belongs to the TetR/AcrR family of transcriptional regulators. P96856 No regulatory proteins 3MNL P96856 P96856 GO:0006350,GO:0003700,GO:0006355 P96856 NC_000962.3 Mycobrowser_v5 CDS 4017089 4018168 . - 0 Rv3575c Rv3575c Involved in transcriptional mechanism. Transcriptional regulatory protein (probably LacI-family) Rv3575c, (MTCY06G11.22c), len: 359 aa. Probable transcriptional regulator belonging to lacI family, similar to others e.g. BAB53947|MLL8376 from Rhizobium loti (Mesorhizobium loti) (358 aa), FASTA scores: opt: 707, E(): 2.6e-35, (35.5% identity in 355 aa overlap); Q9RRI9|DR2501 from Deinococcus radiodurans (359 aa) FASTA scores: opt: 544, E(): 1.6e-25, (40.35% identity in 347 aa overlap); Q9RL31|SCF51A.34 from Streptomyces coelicolor (347 aa), FASTA scores: opt: 307, E(): 2.9e-11, (30.0% identity in 330 aa overlap); O87590|CELR_THEFU from Thermomonospora fusca (340 aa), FASTA scores: opt: 280, E(): 1.2e-09, (32.3% identity in 353 aa overlap); P21867|RAFR_ECOLI from Escherichia coli (335 aa) FASTA scores: opt: 241, E(): 2.6e-07, (27.15% identity in 269 aa overlap); etc. Equivalent to AAK48039 from Mycobacterium tuberculosis strain CDC1551 (404 aa) but shorter 45 aa. Contains possible helix-turn-helix motif, at aa 9-30 (+5.86 SD). Could belong to the LacI family of transcriptional regulators. P96857 No regulatory proteins P96857 P96857 GO:0006355,GO:0006350,GO:0003700,GO:0005622 P96857 NC_000962.3 Mycobrowser_v5 CDS 4018358 4019071 . + 0 Rv3576 lppH Unknown Possible conserved lipoprotein LppH Rv3576, (MTCY06G11.23), len: 237 aa. Possible lppH, conserved lipoprotein, similar in part with proteins from Mycobacterium tuberculosis; C-terminus of Q11053|PKNH_MYCTU|PKNH|Rv1266c|MT1304|MTCY50.16 probable serine/threonine-protein kinase (626 aa) FASTA scores: opt: 396, E(): 6.5e-19, (36.0% identity in 200 aa overlap); and with P71740|LPPR|Rv2403c|MTCY253.17 probable lipoprotein protein (251 aa), FASTA scores: opt: 134, E(): 0.087, (22.7% identity in 207 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Note that previously known as pknM. I6YGJ4 No cell wall and cell processes I6YGJ4 I6YGJ4 NC_000962.3 Mycobrowser_v5 CDS 4052950 4053603 . + 0 Rv3611 Rv3611 Unknown Hypothetical arginine and proline rich protein Rv3611, (MTCY07H7B.11c), len: 217 aa. Hypothetical unknown arg-, pro-rich protein. Possible ORF containing several direct repeats. O06272 No conserved hypotheticals O06272 NC_000962.3 Mycobrowser_v5 CDS 4021425 4022393 . - 0 Rv3579c Rv3579c Causes methylation Possible tRNA/rRNA methyltransferase Rv3579c, (MTCY06G11.26c), len: 322 aa. Possible tRNA/rRNA methyltransferase, equivalent, but longer 31 aa, to Q9CCW4|ML0324 putative methyltransferase from Mycobacterium leprae (278 aa), FASTA scores: opt: 1517, E(): 3.4e-79, (83.75% identity in 277 aa overlap). Also highly similar to Q9L0Q5|SCD8A.09 from Streptomyces coelicolor (314 aa), FASTA scores: opt: 937, E(): 3.4e-46, (56.75% identity in 319 aa overlap); and similar to others e.g. Q06753|YACO_BACSU from Bacillus subtilis (249 aa), FASTA scores: opt: 616, E(): 4.9e-28, (41.05% identity in 246 aa overlap); Q9KGF2|BH0113 from Bacillus halodurans (249 aa), FASTA scores: opt: 596, E(): 6.7e-27, (38.5% identity in 244 aa overlap); P74328|Y955_SYNY3|SLR0955 from Synechocystis sp. strain PCC 6803 (384 aa), FASTA scores: opt: 585, E(): 4e-26, (35.85% identity in 304 aa overlap); P39290|YJFH_ECOLI|B4180 from Escherichia coli strain K12 (243 aa), FASTA scores: opt: 521, E(): 1.2e-22, (38.1% identity in 244 aa overlap); etc. Equivalent to AAK48043 from Mycobacterium tuberculosis strain CDC1551 (253 aa) but longer 69 aa. Possibly belongs to the RNA methyltransferase TrmH family. P9WFY5 No intermediary metabolism and respiration P96861 P9WFY5 GO:0008173,GO:0006396,GO:0003723 2.1.1.- P9WFY5 NC_000962.3 Mycobrowser_v5 CDS 4022394 4023803 . - 0 Rv3580c cysS1 Involved in translation [catalytic activity: ATP + L-cysteine + tRNA(CYS) = AMP + pyrophosphate + L-cysteinyl-tRNA(CYS)]. Cysteinyl-tRNA synthetase 1 CysS1 (cysteine--tRNA ligase 1) (CYSRS 1) (cysteine translase) Rv3580c, (MTCY06G11.27c), len: 469 aa. Probable cysS1, cysteinyl-tRNA synthetase, equivalent to P57990|SYC1_MYCLE|CYSS1|CYSS|ML0323 cysteinyl-tRNA synthetase 1 from Mycobacterium leprae (473 aa) FASTA scores: opt: 2825, E(): 3.4e-172, (86.5% identity in 467 aa overlap). Also similar to many e.g. Q9L0Q6|SCD8A.08 from Streptomyces coelicolor (613 aa), FASTA scores: opt: 1834, E(): 4.7e-109, (57.5% identity in 461 aa overlap); Q9I2U7|CYSS|PA1795 from Pseudomonas aeruginosa (460 aa) FASTA scores: opt: 1197, E(): 1.2e-68, (41.65% identity in 468 aa overlap); P21888|SYC_ECOLI P21888|CYSS|B0526 from Escherichia coli strain K12 (461 aa), FASTA scores: opt: 1189, E(): 4e-68, (43.0% identity in 463 aa overlap); etc. Belongs to class-I aminoacyl-tRNA synthetase family. Strongly similar to methionyl-tRNA synthetase. P9WFW1 No information pathways P0A634 P9WFW1 GO:0004817,GO:0006423,GO:0005737,GO:0046872,GO:0005524 6.1.1.16 P9WFW1 NC_000962.3 Mycobrowser_v5 CDS 4046303 4047496 . - 0 Rv3604c Rv3604c Unknown Probable conserved transmembrane protein rich in alanine and arginine and proline Rv3604c, (MTCY07H7B.18), len: 397 aa. Probable conserved ala-, arg-, pro-rich transmembrane protein, equivalent to O69526|MLCB2548.03c|ML0228 putative membrane protein from Mycobacterium leprae (432 aa), FASTA scores: opt: 869, E(): 2.9e-31, (59.7% identity in 432 aa overlap). Contains two possible membrane-spanning domains. N-terminus shortened since first submission (previously 462 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06278 No cell wall and cell processes O06278 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 4025056 4025544 . - 0 Rv3583c Rv3583c Involved in transcriptional mechanism. Possible transcription factor Rv3583c, (MTV024.01c, MTCY06G11.30c), len: 162 aa. Possible transcriptional factor, identical to Q9CCW7|ML0320 putative transcription factor from Mycobacterium leprae (165 aa), FASTA scores: opt: 1004, E(): 6.1e-56, (97.55% identity in 162 aa overlap); and Q9ZBM8|MLCB1450.01c putative transcriptional regulator from Mycobacterium leprae (94 aa), FASTA scores: opt: 600, E(): 6e-31, (97.85% identity in 94 aa overlap). Also highly similar to others e.g. Q9L0Q9|SCD8A.05 from Streptomyces coelicolor (160 aa), FASTA scores: opt: 878, E(): 4.3e-48, (85.0% identity in 160 aa overlap); Q9K600|BH3935 from Bacillus halodurans (153 aa) FASTA scores: opt: 383, E(): 3.1e-17, (36.4% identity in 151 aa overlap); Q9KD36|BH1383 from Bacillus halodurans (164 aa) FASTA scores: opt: 305, E(): 2.4e-12, (33.55% identity in 164 aa overlap); etc. P9WJG3 No regulatory proteins O53568 P9WJG3 GO:0003700,GO:0006355 P9WJG3 NC_000962.3 Mycobrowser_v5 CDS 4025830 4026378 . + 0 Rv3584 lpqE Unknown Possible conserved lipoprotein LpqE Rv3584, (MTV024.02), len: 182 aa. Possible lpqE, conserved lipoprotein, equivalent to Q9ZBM7|MLCB1450.02|LPQE|ML0319 putative lipoprotein from Mycobacterium leprae (183 aa), FASTA scores: opt: 722, E(): 6.2e-37, (63.45% identity in 175 aa overlap). Also similar in part to Q9KK69 exported protein 996A010 (fragment) from Mycobacterium avium (41 aa), FASTA scores: opt: 180, E(): 0.00012, (69.25% identity in 39 aa overlap); and Q9L0R0|SCD8A.04c putative lipoprotein from Streptomyces coelicolor (241 aa), FASTA scores: opt: 127, E(): 0.86, (27.15% identity in 173 aa overlap). Equivalent to AAK48048 from Mycobacterium tuberculosis strain CDC1551 (238 aa) but shorter 56 aa. Contains probable N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WK63 No cell wall and cell processes P9WK63 GO:0005886 NC_000962.3 Mycobrowser_v5 CDS 4026444 4027886 . + 0 Rv3585 radA Involved in genetic recombination. May play a role in the repair of endogenous alkylation damage. DNA repair protein RadA (DNA repair protein SMS) Rv3585, (MTV024.03), len: 480 aa. Probable radA, DNA repair protein (see citation below), similar to many e.g. Q9X8L5|SCE94.02 from Streptomyces coelicolor (469 aa), FASTA scores: opt: 1607, E(): 3.1e-84, (56.15% identity in 454 aa overlap); Q9JV51|RADA|NMA0992 from Neisseria meningitidis (serogroup A) (459 aa), FASTA scores: opt: 1275, E(): 2.5e-65, (45.0% identity in 458 aa overlap); and Q9K040|RADA|NMB0782 from Neisseria meningitidis (serogroup B) (459 aa), FASTA scores: opt: 1269, E(): 5.4e-65, (44.5% identity in 456 aa overlap); P37572|RADA_BACSU|SMS from Bacillus subtilis (458 aa), FASTA scores: opt: 1204, E(): 2.7e-61, (39.55% identity in 455 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the RadA family. P9WHJ9 No information pathways P65953 P9WHJ9 GO:0004176,GO:0006281,GO:0003684,GO:0046872,GO:0006508,GO:0004252,GO:0005524 P9WHJ9 NC_000962.3 Mycobrowser_v5 CDS 4073634 4075409 . + 0 Rv3635 Rv3635 Unknown Probable conserved transmembrane protein Rv3635, (MTCY15C10.17c), len: 591 aa (start unclear). Probable conserved transmembrane protein, equivalent, but longer 25 aa, to O69545|ML0203|MLCB2548.28 putative membrane protein from Mycobacterium leprae (569 aa), FASTA scores: opt: 2933, E(): 4.6e-173, (77.0% identity in 569 aa overlap). I6Y460 No cell wall and cell processes I6Y460 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 4031404 4033158 . - 0 Rv3590c PE_PGRS58 Function unknown PE-PGRS family protein PE_PGRS58 Rv3590c, (MTV024.08c, MTCY6F7.04), len: 584 aa. PE_PGRS58, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to e.g. O53439|Rv1091|MTV017.44 (853 aa), FASTA scores: opt: 2005, E(): 1.4e-70, (54.95% identity in 646 aa overlap). I6XHM5 No PE/PPE Q7D580 I6XHM5 I6XHM5 NC_000962.3 Mycobrowser_v5 CDS 4027891 4028967 . + 0 Rv3586 Rv3586 Function unknown Conserved hypothetical protein Rv3586, (MTV024.04), len: 358 aa. Conserved hypothetical protein, highly similar to Q9X8L6|SCE94.03 putative DNA-binding protein from Streptomyces coelicolor (374 aa), FASTA scores: opt: 1338, E(): 5e-75, (59.95% identity in 347 aa overlap); P37573|YACK_BACSU hypothetical 40.7 KDA protein from Bacillus subtilis (360 aa), FASTA scores: opt: 875, E(): 1.4e-46, (42.15% identity in 344 aa overlap); Q9KGG0|BH0105 hypothetical protein from Bacillus halodurans (357 aa), FASTA scores: opt: 844, E(): 1.1e-44, (40.3% identity in 350 aa overlap); Q9WY43|TM0200 conserved hypothetical protein from Thermotoga maritima (357 aa), FASTA scores: opt: 735, E(): 5.7e-38, (39.4% identity in 353 aa overlap). Also some similarity with other proteins. Contains probable coiled-coil from 144 to 179. P9WNW5 No conserved hypotheticals O53571 P9WNW5 GO:0006281,GO:0003677 P9WNW5 NC_000962.3 Mycobrowser_v5 CDS 4033269 4034042 . - 0 Rv3591c Rv3591c Function unknown; probably involved in cellular metabolism. Possible hydrolase Rv3591c, (MTCY6F7.03), len: 257 aa. Possible hydrolase, equivalent to Q9CBI9|ML1921 hypothetical protein from Mycobacterium leprae (256 aa) FASTA scores: opt: 1421, E(): 5.6e-83, (78.5% identity in 251 aa overlap). Also similar to others e.g. Q9K3V0|SCD10.27 putative hydrolase from Streptomyces coelicolor (352 aa), FASTA scores: opt: 193, E(): 5.2e-05, (33.35% identity in 270 aa overlap); O33745|STTC thioesterase from Streptomyces sp (308 aa) FASTA scores: opt: 242, E(): 3.6e-08, (30.35% identity in 270 aa overlap); Q9RK95|SCF1.09 putative hydrolase from Streptomyces coelicolor (258 aa), FASTA scores: opt: 239, E(): 4.9e-08, (30.75% identity in 247 aa overlap); Q9HZ14|PA3226 probable hydrolase from Pseudomonas aeruginosa (275 aa), FASTA scores: opt: 226, E(): 3.4e-07, (26.6% identity in 252 aa overlap); Q9HPT9|est|VNG1474G carboxylesterase from Halobacterium sp. strain NRC-1 (274 aa), FASTA scores: opt: 215, E(): 1.7e-06, (26.95% identity in 256 aa overlap); etc. I6YGL1 No intermediary metabolism and respiration O06157 I6YGL1 GO:0016787 3.-.-.- I6YGL1 NC_000962.3 Mycobrowser_v5 CDS 4034057 4034374 . + 0 Rv3592 mhuD Possibly involved in heme degradation pathway. May be involved in iron storage. Possible heme degrading protein MhuD Rv3592, (MTCY6F7.02c), len: 105 aa. Possible mhuD, heme-degrading protein, equivalent to Q9CBI8|ML1922 hypothetical protein from Mycobacterium leprae (105 aa) FASTA scores: opt: 591, E(): 2.5e-34, (84.6% identity in 104 aa overlap). Shows some similarity with other bacterial hypothetical proteins e.g. Q9RXN8|DR0272 from Deinococcus radiodurans (109 aa), FASTA scores: opt: 178, E(): 1e-05, (34.3% identity in 102 aa overlap); P38049|YHGC_BACSU from Bacillus subtilis (166 aa) FASTA scores: opt: 175, E(): 2.4e-05, (40.85% identity in 71 aa overlap); Q9K649|BH3883 from Bacillus halodurans (102 aa) FASTA scores: opt: 162, E(): 0.00012, (33.75% identity in 80 aa overlap); etc. P9WKH3 No intermediary metabolism and respiration 3HX9 O06156 P9WKH3 GO:0005737,GO:0016491,GO:0017000 P9WKH3 NC_000962.3 Mycobrowser_v5 CDS 4047705 4048181 . - 0 Rv3605c Rv3605c Unknown Probable conserved secreted protein Rv3605c, (MTCY07H7B.17), len: 158 aa. Probable conserved secreted or membrane protein, identical to O69527|MLCB2548.04c|ML0227 putative membrane protein from Mycobacterium leprae (158 aa), FASTA scores: opt: 944, E(): 2.6e-56, (85.45% identity in 158 aa overlap). Also similar to other proteins e.g. Q9X8I2|SCE9.09 possible secreted protein from Streptomyces coelicolor (162 aa), FASTA scores: opt: 174, E(): 9.2e-05, (31.25% identity in 128 aa overlap); etc. Contains possible N-terminal signal sequence. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06277 No cell wall and cell processes O06277 O06277 O06277 NC_000962.3 Mycobrowser_v5 CDS 4043862 4044281 . - 0 Rv3601c panD Involved in pantothenate biosynthesis [catalytic activity: L-aspartate = beta-alanine + CO(2)]. Probable aspartate 1-decarboxylase precursor PanD (aspartate alpha-decarboxylase) Rv3601c, (MTCY07H7B.21), len: 139 aa. Probable panD, aspartate 1-decarboxylase, identical to Q9CD57|PAND|ML0231 putative aspartate-1-decarboxylase from Mycobacterium leprae (142 aa), FASTA scores: opt: 733, E(): 5.5e-41, (82.85% identity in 140 aa overlap). Also highly similar to many e.g. CAC44328|PAND from Streptomyces coelicolor (139 aa), FASTA scores: opt: 578, E(): 6.4e-31, (75.0% identity in 120 aa overlap); Q9X4N0|PAND from Corynebacterium glutamicum (Brevibacterium flavum) (136 aa), FASTA scores: opt: 506, E(): 3e-26, (62.2% identity in 135 aa overlap); P52999|PAND_BACSU from Bacillus subtilis (127 aa) FASTA scores: opt: 421, E(): 9.6e-21, (54.75% identity in 123 aa overlap); P31664|PAND_ECOLI|B0131 from Escherichia coli strain K12 (126 aa), FASTA scores: opt: 388, E(): 1.3e-18, (50.45% identity in 113 aa overlap); etc. P9WIL3 No intermediary metabolism and respiration 2C45 P65660 P9WIL3 GO:0004068,GO:0005737,GO:0015940,GO:0006523 4.1.1.11 P9WIL3 NC_000962.3 Mycobrowser_v5 ncRNA 4040879 4040938 . + 0 MTB000070 mpr17 Unknown Fragment of putative small regulatory RNA mpr17, fragment of putative small regulatory RNA (See DiChiara et al., 2010), ends not mapped, 82-118 nt bands detected by Northern blot in M. bovis BCG Pasteur. No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 4035857 4036684 . + 0 Rv3594 Rv3594 Function unknown Conserved hypothetical protein Rv3594, (MTCY07H7B.28c), len: 275 aa. Hypothetical protein, highly similar in part with Q9ZX49|GP29 from Mycobacteriophage TM4 (547 aa), FASTA scores: opt: 526, E(): 1.3e-25, (46.25% identity in 186 aa overlap); and Q9FZS0|LYSA|GP2 from Mycobacterium phage Ms6 (384 aa) FASTA scores: opt: 147, E(): 0.064, (33.35% identity in 84 aa overlap). I6Y3Z2 No conserved hypotheticals O06288 I6Y3Z2 GO:0009253,GO:0008745 I6Y3Z2 NC_000962.3 Mycobrowser_v5 CDS 4038158 4040704 . - 0 Rv3596c clpC1 Hydrolyses proteins in presence of ATP. May interact with a CLPP-like protease involved in degradation of denatured proteins. Probable ATP-dependent protease ATP-binding subunit ClpC1 Rv3596c, (MTCY07H7B.26), len: 848 aa. Probable clpC1, ATP-dependent protease ATP-binding subunit, equivalent to P24428|CLPC_MYCLE probable ATP-dependent CLP protease ATP-binding subunit from Mycobacterium leprae (848 aa) (see Misra et al., 1996), FASTA scores: opt: 5286, E(): 0, (97.15% identity in 845 aa overlap). Also highly similar to members of the clpA/clpB family e.g. Q9S6T8|SCE94.24c from Streptomyces coelicolor (841 aa) FASTA scores: opt: 4399, E(): 0, (81.0% identity in 848 aa overlap); Q9KGG2|CLPC|BH0103 from Bacillus halodurans (813 aa), FASTA scores: opt: 3279, E(): 3.8e-173, (61.9% identity in 808 aa overlap); Q55662|CLPC|SLL0020 from Synechocystis sp. strain PCC 6803 (821 aa), FASTA scores: opt: 3201, E(): 7.6e-169, (60.5% identity in 820 aa overlap); P51332|CLPC_PORPU from Porphyra purpurea (821 aa), FASTA scores: opt: 3045, E(): 3e-160, (57.65% identity in 817 aa overlap); P37571|CLPC_BACSU|MECB from Bacillus subtilis (810 aa), FASTA scores: opt: 2969, E(): 4.6e-156, (61.15% identity in 811 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Note that previously known as clpC. Belongs to the CLPA/CLPB family, CLPC subfamily. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P9WPC9 No intermediary metabolism and respiration P0A522 P9WPC9 GO:0003677,GO:0004518,GO:0017111,GO:0006289,GO:0005515,GO:0019538,GO:0005524 P9WPC9 NC_000962.3 Mycobrowser_v5 CDS 4043041 4043859 . - 0 Rv3600c Rv3600c Function unknown Conserved protein Rv3600c, (MTCY07H7B.22), len: 272 aa. Conserved protein, identical to Q9CD56|ML0232 hypothetical protein from Mycobacterium leprae (274 aa), FASTA scores: opt: 1585, E(): 1.3e-92, (90.5% identity in 274 aa overlap). Also highly similar to others e.g. Q9X8N6|SCE94.31c from Streptomyces coelicolor (265 aa) FASTA scores: opt: 878, E(): 3.9e-48, (51.5% identity in 268 aa overlap); and Q9KGH5|BH0086 from Bacillus halodurans (254 aa), FASTA scores: opt: 611, E(): 2.4e-31, (37.5% identity in 264 aa overlap). And similar to various bacterial proteins e.g. Q9F985 putative 32 KDA replication protein from Bacillus stearothermophilus (258 aa), FASTA scores: opt: 594, E(): 2.8e-30, (37.45% identity in 267 aa overlap); P37564|YACB_BACSU from Bacillus subtilis (233 aa), FASTA scores: opt: 522, E(): 8.8e-26, (38.95% identity in 213 aa overlap); Q9RX54|DR0461 conserved hypothetical protein from Deinococcus radiodurans (262 aa), FASTA scores: opt: 503, E(): 1.5e-24, (38.45% identity in 268 aa overlap); etc. P9WPA1 No conserved hypotheticals O06282 P9WPA1 GO:0015937,GO:0005737,GO:0046872,GO:0004594,GO:0045941,GO:0016563,GO:0005524 2.7.1.33 P9WPA1 NC_000962.3 Mycobrowser_v5 CDS 4060295 4060591 . - 0 Rv3620c esxW Unknown Putative ESAT-6 like protein EsxW (ESAT-6 like protein 10) Rv3620c, (MTCY15C10.32, MTCY07H7B.02, MT3722), len: 98 aa. EsxW, ESAT-6 like protein (see citation below). Member of the M. tuberculosis hypothetical QILSS protein family with Rv1038c, Rv1792, Rv2347c and Rv1197|O05299|ES63_MYCTU|MT1235|MTCI364.09 putative ESAT-6 like protein 3 from Mycobacterium tuberculosis (98 aa), FASTA scores: opt: 638, E(): 2.3e-36, (97.95% identity in 98 aa overlap). Also similar to Q49945|ES6Y_MYCLE putative ESAT-6 like protein Y from Mycobacterium leprae (100 aa), FASTA scores: opt: 370, E(): 2.1e-18, (57.9% identity in 95 aa overlap); etc. Belongs to the ESAT6 family. P9WNI3 No cell wall and cell processes P9WNI3 P9WNI3 NC_000962.3 Mycobrowser_v5 CDS 4045207 4046118 . - 0 Rv3603c Rv3603c Function unknown Conserved hypothetical alanine and leucine rich protein Rv3603c, (MTCY07H7B.19), len: 303 aa. Conserved hypothetical ala-, leu-rich protein, identical except at N-terminus (really different) to AAK48066|MT3708 chalcone/stilbene synthase family protein from Mycobacterium tuberculosis strain CDC1551 (361 aa) FASTA scores: opt: 1742, E(): 8.3e-95, (100.0% identity in 275 aa overlap). Equivalent to O69525|MLCB2548.02c|ML0229 hypothetical 32.7 KDA protein from Mycobacterium leprae (309 aa), FASTA scores: opt: 947, E(): 2.4e-48, (67.85% identity in 311 aa overlap). Also highly similar to Q9X845|SCE126.02c hypothetical 42.2 KDA protein from Streptomyces coelicolor (420 aa), FASTA scores: opt: 683, E(): 8.5e-33, (49.3% identity in 284 aa overlap). O06279 No conserved hypotheticals O06279 O06279 GO:0003824,GO:0008152 O06279 NC_000962.3 Mycobrowser_v5 CDS 4077884 4078450 . - 0 Rv3639c Rv3639c Function unknown Conserved hypothetical protein Rv3639c, (MTCY15C10.13), len: 188 aa. Hypothetical protein, with C-terminus highly similar to N-terminus of P95044|Rv0698|MTCY210.15 hypothetical 22.3 KDA protein from Mycobacterium tuberculosis (203 aa), FASTA scores: opt: 224, E(): 4.5e-07, (54.8% identity in 73 aa overlap). I6YGU1 No conserved hypotheticals I6YGU1 I6YGU1 NC_000962.3 Mycobrowser_v5 CDS 4060648 4061889 . - 0 Rv3621c PPE65 Function unknown PPE family protein PPE65 Rv3621c, (MTCY15C10.31, MTCY07H7B.01), len: 413 aa. PPE65, Member of the Mycobacterium tuberculosis PPE family, ala-, gly-rich proteins, similar to many e.g. Q10813|YS92_MYCTU|Rv2892c|MT2959|MTCY274.23c (408 aa) FASTA scores: opt: 955, E(): 1.8e-42, (44.45% identity in 423 aa overlap). P9WHX3 No PE/PPE Q7D569 P9WHX3 P9WHX3 NC_000962.3 Mycobrowser_v5 CDS 4075752 4076099 . + 0 Rv3636 Rv3636 Possibly required for the transposition of an insertion element. Possible transposase Rv3636, (MTCY15C10.16c), len: 115 aa. Possible transposase, weakly similar to others e.g. O69924|SC3C8.12 putative transposase from Streptomyces coelicolor (487 aa) FASTA scores: opt: 132, E(): 0.12, (33.05% identity in 112 aa overlap); O96916 TC1-like transposase from Anopheles gambiae (African malaria mosquito) (332 aa), FASTA scores: opt: 117, E(): 0.84, (30.75% identity in 91 aa overlap); Q9R2U5|IS466A|IS466A-ORF|TNPA|IS469|SCP1.276 transposase (insertion element IS466S transposase) from Streptomyces coelicolor (513 aa), FASTA scores: opt: 114, E(): 2, (30.5% identity in 82 aa overlap); etc. Similar in part to P96288|Rv2943|MTCY24G1.06c hypothetical 45.8 KDA protein from Mycobacterium tuberculosis (413 aa), FASTA scores: opt: 533, E(): 1.4e-28, (74.55% identity in 110 aa overlap). Contains possible helix-turn-helix motif from aa 19-40 (+4.98 SD). O06371 No insertion seqs and phages O06371 O06371 GO:0016987,GO:0003700,GO:0006352,GO:0006355 NC_000962.3 Mycobrowser_v5 CDS 4049977 4050585 . - 0 Rv3609c folE Involved in the biosynthesis of tetrahydrofolate (at the first step) [catalytic activity: GTP + 2 H(2)O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)dihydropteridine triphosphate]. GTP cyclohydrolase I FolE (GTP-ch-I) Rv3609c, (MTCY07H7B.13), len: 202 aa. Probable folE (alternate gene name: gchA), GTP cyclohydrolase I, equivalent to O69531|GCH1_MYCLE|FOLE|ML0223|MLCB2548.08c GTP cyclohydrolase I from Mycobacterium leprae (205 aa) FASTA scores: opt: 1112, E(): 3.8e-63, (81.95% identity in 205 aa overlap). Also highly similar to many e.g. Q9X8I3|GCH1_STRCO|FOLE|SCE9.10c from Streptomyces coelicolor (201 aa), FASTA scores: opt: 873, E(): 4.2e-48, (67.4% identity in 187 aa overlap); Q9KCC7|MTRA|BH1646 from Bacillus halodurans (188 aa), FASTA scores: opt: 757, E(): 8.1e-41, (62.3% identity in 183 aa overlap); P19465|GCH1_BACSU|FOLE|MTRA from Bacillus subtilis (190 aa), FASTA scores: opt: 750, E(): 2.3e-40, (58.95% identity in 190 aa overlap); etc. Contains PS00860 GTP cyclohydrolase I signature 2. Belongs to the GTP cyclohydrolase I family. P9WN57 No intermediary metabolism and respiration P64207 P9WN57 GO:0003934,GO:0005737,GO:0046872,GO:0006730,GO:0046654,GO:0005525 3.5.4.16 P9WN57 NC_000962.3 Mycobrowser_v5 CDS 4053518 4053847 . - 0 Rv3612c Rv3612c Function unknown Conserved hypothetical protein Rv3612c, (MTCY07H7B.10), len: 109 aa. Conserved hypothetical protein. Residues 58 to 81 highly similar to N-terminal part of AAK46718|MT2424 hypothetical 3.9 KDA protein from Mycobacterium tuberculosis strain CDC1551 (36 aa), FASTA scores: opt: 108, E(): 0.38, (69.25% identity in 26 aa overlap). I6YGR2 No conserved hypotheticals I6YGR2 NC_000962.3 Mycobrowser_v5 CDS 4080571 4080765 . - 0 Rv3642c Rv3642c Unknown Hypothetical protein Rv3642c, (MTCY15C10.10), len: 64 aa. Hypothetical unknown protein. I6Y464 No conserved hypotheticals I6Y464 I6Y464 NC_000962.3 Mycobrowser_v5 CDS 4081160 4081351 . + 0 Rv3643 Rv3643 Unknown Hypothetical protein Rv3643, (MTCY15C10.09c), len: 63 aa (questionable ORF). Identical to AAK48106 from Mycobacterium tuberculosis strain CDC1551 (33 aa) but longer 30 aa. O06364 No conserved hypotheticals O06364 NC_000962.3 Mycobrowser_v5 CDS 4054812 4055123 . - 0 Rv3615c espC Function unknown ESX-1 secretion-associated protein EspC Rv3615c, (MTCY07H7B.07), len: 103 aa. EspC, ESX-1 secretion-associated protein, equivalent to Q49723|ML0406|B1620_C2_214|MLCL383 hypothetical 11.1 KDA protein from Mycobacterium leprae (106 aa), FASTA scores: opt: 364, E(): 4.1e-18, (60.85% identity in 92 aa overlap). Also shows similarity to P96212|Rv3865|MTCY01A6.03 hypothetical 10.6 KDA protein from Mycobacterium tuberculosis (103 aa), FASTA scores: opt: 198, E(): 6.8e-07, (36.25% identity in 102 aa overlap). Has been shown to interact with itself, by yeast two-hybrid analysis (See MacGurn et al., 2005). P9WJD7 No cell wall and cell processes P65087 P9WJD7 GO:0009405,GO:0005576 NC_000962.3 Mycobrowser_v5 CDS 4057733 4058701 . + 0 Rv3617 ephA Biotransformation enzyme that catalyzes the hydrolysis of epoxides (alkene oxides, oxiranes) and arene oxides to less reactive and more water soluble dihydrodiols by the trans addition of water. Thought to be involved in detoxification reactions following oxidative damage to lipids [catalytic activity: an epoxide + H(2)O = a glycol]. Probable epoxide hydrolase EphA (epoxide hydratase) (arene-oxide hydratase) Rv3617, (MTCY07H7B.05c, MTCY15C10.35c), len: 322 aa. Probable ephA, epoxide hydrolase (see citation below), similar to many e.g. Q9A8W9|CC1229 from Caulobacter crescentus (330 aa), FASTA scores: opt: 965, E(): 1.8e-51, (46.15% identity in 323 aa overlap); Q9M9W5|F18C1.13 from Arabidopsis thaliana (Mouse-ear cress) (331 aa), FASTA scores: opt: 778, E(): 4.3e-40, (40.35% identity in 332 aa overlap); Q9S7P1 from Oryza sativa (Rice) (322 aa), FASTA scores: opt: 774, E(): 7.4e-40, (41.1% identity in 321 aa overlap); P80299|HYES_RAT|EPHX2 from Rattus norvegicus (Rat) (554 aa), FASTA scores: opt: 759, E(): 9.5e-39, (40.5% identity in 306 aa overlap) (similarity only with the C-terminal part for this one); etc. Similar to alpha/beta hydrolase fold. Contains PS00888 Cyclic nucleotide-binding domain signature 1. I6YGS0 No virulence, detoxification, adaptation O06266 I6YGS0 GO:0033961 3.3.2.3,3.3.2.9 I6YGS0 NC_000962.3 Mycobrowser_v5 CDS 4059984 4060268 . - 0 Rv3619c esxV Unknown Putative ESAT-6 like protein EsxV (ESAT-6 like protein 1) Rv3619c, (MTCY15C10.33, MTCY07H7B.03, MT3721), len: 94 aa. EsxV, ESAT-6 like protein (see citations below), highly similar to many Mycobacterial ESAT-6 like proteins e.g. O53942|ES65_MYCTU putative ESAT-6 like protein 5 from Mycobacterium tuberculosis (94 aa), FASTA scores: opt: 582, E(): 4.4e-33, (92.55% identity in 94 aa overlap); Q49946|ES6X_MYCLE|U1756D putative ESAT-6 like protein X from Mycobacterium leprae (95 aa), FASTA scores: opt: 409, E(): 2.5e-21, (64.15% identity in 92 aa overlap); etc. Strictly identical to P96364|ES61_MYCTU|Rv1037c|MT1066|MTCY10G2.12 putative ESAT-6 like protein 1 (94 aa). Belongs to the ESAT6 family. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P0DOA7 No cell wall and cell processes P0DOA7 P0DOA7 NC_000962.3 Mycobrowser_v5 CDS 4062527 4063249 . + 0 Rv3623 lpqG Unknown Probable conserved lipoprotein LpqG Rv3623, (MTCY15C10.29c), len: 240 aa. Probable lpqG, conserved lipoprotein, showing some similarity with hypothetical proteins e.g. Q57432 from Methanosarcina barkeri (251 aa), FASTA scores: opt: 319, E(): 6.8e-12, (31.2% identity in 218 aa overlap); Q9PEA5|XF1123 outer membrane protein from Xylella fastidiosa (242 aa) FASTA scores: opt: 312, E(): 1.7e-11, (28.25% identity in 237 aa overlap); BAB49547|MLR2408 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (236 aa), FASTA scores: opt: 304, E(): 5e-11, (27.05% identity in 244 aa overlap); etc. Has suitable signal peptide and prokaryotic membrane lipoprotein lipid attachment site (PS00013). I6X7X3 No cell wall and cell processes O06384 I6X7X3 I6X7X3 NC_000962.3 Mycobrowser_v5 CDS 4063901 4064872 . - 0 Rv3625c mesJ Thought to be involved in a cell cycle process. Possible cell cycle protein MesJ Rv3625c, (MT3727, MTCY15C10.27), len: 323 aa. Possible mesJ, cell cycle protein, equivalent to O69538|Y0C5_MYCLE|ML0213|MLCB2548.18c hypothetical 34.1 KDA protein from Mycobacterium leprae (323 aa) FASTA scores: opt: 1592, E(): 9e-92, (78.0% identity in 327 aa overlap). Similar to bacterial hypothetical proteins Q9X8I6|SCE9.13c from Streptomyces coelicolor (352 aa) FASTA scores: opt: 705, E(): 1.4e-36, (47.85% identity in 305 aa overlap); and Q9HXZ3|PA3638 from Pseudomonas aeruginosa (442 aa), FASTA scores: opt: 382, E(): 2e-16, (40.6% identity in 271 aa overlap). But also similar (or with similarity) to bacterial cell cycle proteins (MESJ) e.g. Q9KPX0|VC2242 MESJ protein from Vibrio cholerae (440 aa), FASTA scores: opt: 363, E(): 3e-15, (34.8% identity in 253 aa overlap); Q9RV23|DR1207 (600 aa) cell cycle protein MESJ (putative/cytosine deaminase-related protein) from Deinococcus radiodurans (600 aa), FASTA scores: opt: 310, E(): 7.6e-12, (36.6% identity in 265 aa overlap) (similar only at the N-terminal end); Q9PFJ8|XF0659 cell cycle protein from Xylella fastidiosa (437 aa), FASTA scores: opt: 301, E(): 2.1e-11, (35.05% identity in 271 aa overlap); P52097|MESJ_ECOLI|B0188 putative cell cycle protein MESJ from Escherichia coli strain K12(432 aa) FASTA scores: opt: 299, E(): 2.8e-11, (34.65% identity in 277 aa overlap); etc. Belongs to the UPF0072 (MESJ/YCF62) family. P9WG53 No cell wall and cell processes P67151 P9WG53 GO:0005737,GO:0016879,GO:0006400,GO:0006412,GO:0005524 6.3.4.- P9WG53 NC_000962.3 Mycobrowser_v5 CDS 4064851 4065903 . - 0 Rv3626c Rv3626c Function unknown Conserved hypothetical protein Rv3626c, (MTCY15C10.26), len: 350 aa. Conserved hypothetical protein, similar to Q9X8I7|SCE9.14c hypothetical protein from Streptomyces coelicolor (375 aa) FASTA scores: opt: 720, E(): 2.2e-38, (41.55% identity in 361 aa overlap); and shows some similarity to Q9HPS0|VNG1497C hypothetical protein (317 aa) FASTA scores: opt: 226, E(): 4.5e-07, (29.7% identity in 347 aa overlap). Contains neutral zinc metallopeptidases, zinc-binding region signature (PS00142). O06381 No conserved hypotheticals O06381 O06381 O06381 NC_000962.3 Mycobrowser_v5 CDS 4067957 4069054 . - 0 Rv3629c Rv3629c Unknown Probable conserved integral membrane protein Rv3629c, (MTCY15C10.23), len: 365 aa. Probable conserved integral membrane protein, equivalent to O69543|MLCB2548.26|ML0205 putative membrane protein from Mycobacterium leprae (356 aa), FASTA scores: opt: 1547, E(): 3e-89, (66.2% identity in 361 aa overlap). Also similar to other membrane and hypothetical proteins e.g. CAC37534|SCIF3.15c putative integral membrane protein from Streptomyces coelicolor (363 aa), FASTA scores: opt: 819, E(): 7.7e-44, (51.55% identity in 351 aa overlap); Q9CGK3|YKJK hypothetical protein from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (339 aa) FASTA scores: opt: 683, E(): 2.2e-35, (48.3% identity in 350 aa overlap); Q9KY24|SCC8A.24c putative integral membrane protein from Streptomyces coelicolor (380 aa) FASTA scores: opt: 528, E(): 1.1e-25, (50.25% identity in 372 aa overlap); Q9RJH8|SCF73.09 putative integral membrane protein from Streptomyces coelicolor (370 aa) FASTA scores: opt: 439, E(): 3.9e-20, (50.2% identity in 384 aa overlap); Q9PE36|XF1192 integral membrane protein from Xylella fastidiosa (341 aa), FASTA scores: opt: 337, E(): 8.3e-14, (47.65% identity in 361 aa overlap); etc. O06378 No cell wall and cell processes O06378 O06378 NC_000962.3 Mycobrowser_v5 CDS 4079925 4080560 . - 0 Rv3641c fic Could be involved in cell filamentation induced by cyclic AMP and may have some role in cell division. Possible cell filamentation protein Fic Rv3641c, (MTCY15C10.11), len: 211 aa. Possible fic, cell filamentation protein, similar to others e.g. Q9PCU8|XF1657 cell filamentation protein from Xylella fastidiosa (203 aa), FASTA scores: opt: 324, E(): 2.2e-14, (32.8% identity in 189 aa overlap); P20605|FIC_ECOLI|B3361 from Escherichia coli strain K12 (200 aa), FASTA scores: opt: 323, E(): 2.5e-14, (31.0% identity in 187 aa overlap); P20751|FIC_SALTY from Salmonella typhimurium (200 aa), FASTA scores: opt: 322, E(): 2.9e-14, (32.65% identity in 193 aa overlap); etc. I6YCN3 No cell wall and cell processes O06366 I6YCN3 I6YCN3 NC_000962.3 Mycobrowser_v5 CDS 4071791 4072666 . + 0 Rv3633 Rv3633 Function unknown Conserved protein Rv3633, (MTCY15C10.19c), len: 291 aa. Conserved protein, similar to Q9X5S6|MMCH from Streptomyces lavendulae (254 aa), FASTA scores: opt: 368, E(): 3.2e-16, (35.05% identity in 194 aa overlap); Q9APW1 hypothetical 32.7 KDA protein from Pseudomonas aeruginosa (295 aa), FASTA scores: opt: 359, E(): 1.3e-15, (37.65% identity in 170 aa overlap); Q9APV4 hypothetical 34.1 KDA protein from Pseudomonas aeruginosa (309 aa), FASTA scores: opt: 316, E(): 7.6e-13, (28.65% identity in 262 aa overlap). And some similarity to Q9HGD7|FUM9 FUM9P from Gibberella moniliformis (300 aa), FASTA scores: opt: 254, E(): 6.5e-09, (29.95% identity in 157 aa overlap); and P47181|YJ9S_YEAST|YJR154W|J2240 hypothetical 39.0 KDA protein from Saccharomyces cerevisiae (Baker's yeast) (346 aa), FASTA scores: opt: 190, E(): 8.5e-05, (26.75% identity in 127 aa overlap). Also similar to P71782|YF01_MYCTU|Rv1501|MT1550|MTCY277.23 from Mycobacterium tuberculosis (273 aa), FASTA scores: opt: 286, E(): 5.5e-11, (27.5% identity in 280 aa overlap). P9WI89 No conserved hypotheticals P67772 P9WI89 P9WI89 NC_000962.3 Mycobrowser_v5 CDS 4078520 4079749 . - 0 Rv3640c Rv3640c Required for the transposition of an insertion sequence. Probable transposase Rv3640c, (MTCY15C10.12), len: 409 aa. Probable transposase, highly similar to others e.g. Q48882 transposase from Mycobacterium avium (411 aa) FASTA scores: opt: 1574, E(): 6.2e-93, (59.75% identity in 400 aa overlap); Q9AKV5 putative transposase (fragment) from Mycobacterium paratuberculosis (395 aa), FASTA scores: opt: 1566, E(): 1.9e-92, (60.0% identity in 395 aa overlap); Q48368 transposase from Mycobacterium avium (410 aa), FASTA scores: opt: 1561, E(): 4.1e-92, (59.4% identity in 404 aa overlap); etc. O06367 No insertion seqs and phages O06367 O06367 GO:0004803,GO:0006313,GO:0003677 O06367 NC_000962.3 Mycobrowser_v5 CDS 4091841 4092878 . + 0 Rv3651 Rv3651 Function unknown Conserved hypothetical protein Rv3651, (MTCY15C10.01c), len: 345 aa. Hypothetical protein, with some similarity to Q9ZHK1 hypothetical 36.5 KDA protein from Rhodococcus sp. X309 (329 aa) FASTA scores: opt: 332, E(): 3.4e-13, (27.4% identity in 321 aa overlap). I6YCP0 No conserved hypotheticals I6YCP0 I6YCP0 NC_000962.3 Mycobrowser_v5 CDS 4130357 4131712 . + 0 Rv3689 Rv3689 Unknown Probable conserved transmembrane protein Rv3689, (MTV025.037), len: 451 aa. Probable conserved transmembrane protein, with Proline rich N-terminus, similar to Q9KYW6|SCE33.17 putative integral membrane protein from Streptomyces coelicolor (462 aa) FASTA scores: opt: 730, E(): 2.7e-21, (38.1% identity in 412 aa overlap). I6YCR8 No cell wall and cell processes I6YCR8 GO:0016021 NC_000962.3 Mycobrowser_v5 ncRNA 4093468 4093522 . + 0 MTB000071 mpr18 Unknown Fragment of putative small regulatory RNA mpr18, fragment of putative small regulatory RNA (See DiChiara et al., 2010), ends not mapped, 82 and 100 nt bands detected by Northern blot in M. bovis BCG Pasteur. No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 4072667 4073611 . - 0 Rv3634c galE1 Involved in galactofuranosyl biosynthesis: converts UDO-GlcP to UDP-GalP [catalytic activity: UDP-glucopyranose = UDP-galactopyranose]. UDP-glucose 4-epimerase GalE1 (galactowaldenase) (UDP-galactose 4-epimerase) (uridine diphosphate galactose 4-epimerase) (uridine diphospho-galactose 4-epimerase) Rv3634c, (MTCY15C10.18), len: 314 aa. GalE1, UDP-glucose 4-epimerase (see citations below), equivalent to O69544|ML0204|RMLB2|MLCB2548.27c putative sugar dehydratase (putative sugar-nucleotide dehydratase) from Mycobacterium leprae (319 aa), FASTA scores: opt: 1798, E(): 8.2e-100, (86.4% identity in 309 aa overlap). Also similar to other UDP-glucose 4-epimerases e.g. Q9WYX9|TM0509 from Thermotoga maritima (309 aa) FASTA scores: opt: 877, E(): 4.8e-45, (45.8% identity in 308 aa overlap); Q57664|GALE_METJA|MJ0211 from Methanococcus jannaschii (305 aa), FASTA scores: opt: 792, E(): 5.4e-40, (42.05% identity in 309 aa overlap); Q9K6S7|BH3649 from Bacillus halodurans (311 aa), FASTA scores: opt: 723, E(): 7e-36, (40.5% identity in 316 aa overlap); Q9HSV1|GALE2|VNG0063G from Halobacterium sp. strain NRC-1 (328 aa), FASTA scores: opt: 597, E(): 2.3e-28, (36.35% identity in 322 aa overlap); etc. Contains short-chain alcohol dehydrogenase family signature (PS00061) but this maynot be significant. Belongs to the sugar epimerase family. Note that previously known as rmlB2, a dTDP-glucose 4,6-dehydratase (see Ma et al., 2001). P9WN67 No intermediary metabolism and respiration Q6MWV3 P9WN67 GO:0044237,GO:0050662,GO:0003978 5.1.3.2 P9WN67 NC_000962.3 Mycobrowser_v5 CDS 4144913 4145614 . - 0 Rv3702c Rv3702c Function unknown Conserved hypothetical protein Rv3702c, (MTV025.050c), len: 233 aa. Conserved hypothetical protein, highly similar to other hypothetical proteins Q9RCZ9|SCM1.45 from Streptomyces coelicolor (271 aa), FASTA scores: opt: 383, E(): 2.3e-17, (44.85% identity in 252 aa overlap); and P54004|Y199_SYNY3|SLR0199 from Synechocystis sp. strain PCC 6803 (304 aa), FASTA scores: opt: 292, E(): 1.7e-11, (30.05% identity in 263 aa overlap); and similar to others e.g. Q9KMU4|VCA0225 from Vibrio cholerae (254 aa), FASTA scores: opt: 260, E(): 1.6e-09, (29.8% identity in 245 aa overlap). Equivalent to AAK48172 from Mycobacterium tuberculosis strain CDC1551 (194 aa) but longer 39 aa. O69670 No conserved hypotheticals O69670 O69670 NC_000962.3 Mycobrowser_v5 CDS 4082807 4084456 . + 0 Rv3645 Rv3645 Unknown. Inhibited by polyphosphates. Probable conserved transmembrane protein Rv3645, (MTCY15C10.07c), len: 549 aa. Probable conserved transmembrane protein, equivalent, but longer 20 aa, to O69547|ML0201|MLCB2548.30 putative membrane protein from Mycobacterium leprae (530 aa), FASTA scores: opt: 2958, E(): 1.5e-168, (85.5% identity in 530 aa overlap). Also closely related to several other hypothetical M. tuberculosis proteins, e.g. Q10631|YD18_MYCTU|Rv1318c|MT1359|MTCY130.03c (541 aa) FASTA scores: opt: 1105, E(): 2.7e-58, (39.35% identity in 506 aa overlap); Q10633|YD20_MYCTU|Rv1320c|MT1362|MTCY130.05c (567 aa) FASTA scores: opt: 1031, E(): 7.1e-54, (38.1% identity in 509 aa overlap); Q10632|YD19_MYCTU|Rv1319c|MTCY130.04c (535 aa), FASTA scores: opt: 1016, E(): 5.3e-53, (37.1% identity in 531 aa overlap); etc. Also similar at C-terminal end to many adenylate cyclases e.g. O83498|TP0485 from Treponema pallidum (614 aa) FASTA scores: opt: 365, E(): 3.2e-14, (31.55% identity in 317 aa overlap); P94180|CYAA from Anabaena sp. strain PCC 7120 (735 aa), FASTA scores: opt: 364, E(): 4.2e-14, (32.75% identity in 229 aa overlap); etc. I6X7Z3 No cell wall and cell processes O06362 I6X7Z3 GO:0016021,GO:0016849,GO:0004871,GO:0007165,GO:0009190 I6X7Z3 NC_000962.3 Mycobrowser_v5 CDS 4084453 4087257 . - 0 Rv3646c topA The reaction catalyzed by topoisomerases LEADS to the conversion of one topological isomer of DNA to another. [catalytic activity: ATP-independent breakage of single-stranded DNA, followed by passage and rejoining]. DNA topoisomerase I TopA (omega-protein) (relaxing enzyme) (untwisting enzyme) (swivelase) (type I DNA topoisomerase) (nicking-closing enzyme) (TOPO I) Rv3646c, (MTCY15C10.06), len: 934 aa. TopA, DNA topoisomerase I (see citations below), equivalent to O69548|TOP1_MYCLE|TOPA|ML0200|MLCB2548.31c DNA topoisomerase I from Mycobacterium leprae (947 aa) FASTA scores: opt: 5150, E(): 0, (84.6% identity in 936 aa overlap). Also highly similar to many e.g. Q9X909|TOP1_STRCO|TOPA|SCH5.06c from Streptomyces coelicolor (952 aa), FASTA scores: opt: 2754, E(): 1.3e-153, (61.3% identity in 928 aa overlap); P73810|TOP1_SYNY3|TOPA|SLR2058 from Synechocystis sp. strain PCC 6803 (898 aa), FASTA scores: opt: 1442, E(): 9.1e-77, (47.15% identity in 927 aa overlap); P47368|TOP1_MYCGE|TOPA|MG122 from Mycoplasma genitalium (709 aa), FASTA scores: opt: 865, E(): 4.8e-43, (30.3% identity in 736 aa overlap); P06612|TOP1_ECOLI|TOPA|SUPX|B1274 from Escherichia coli strain K12 (865 aa), FASTA scores: opt: 397, E(): 0, (39.6% identity in 704 aa overlap); etc. Belongs to prokaryotic type I/III topoisomerase family. P9WG49 No information pathways P0A620 P9WG49 GO:0003917,GO:0006265,GO:0006268,GO:0005524 5.99.1.2 P9WG49 NC_000962.3 Mycobrowser_v5 CDS 4087610 4088188 . - 0 Rv3647c Rv3647c Function unknown Conserved hypothetical protein Rv3647c, (MTCY15C10.05), len: 192 aa. Conserved hpothetical protein, equivalent to O69549|MLCB2548.32c|ML0199 conserved hypothetical protein from Mycobacterium leprae (200 aa), FASTA scores: opt: 1029, E(): 9e-58, (80.4% identity in 199 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). I6Y469 No conserved hypotheticals I6Y469 I6Y469 NC_000962.3 Mycobrowser_v5 CDS 4094660 4094914 . - 0 Rv3654c Rv3654c Function unknown Conserved hypothetical protein Rv3654c, (MTV025.002c), len: 84 aa. Hypothetical protein, similar to C-terminus of Q9X916|SCH5.14c membrane spanning protein from Streptomyces coelicolor (230 aa) FASTA scores: opt: 176, E(): 2.4e-05, (47.0% identity in 83 aa overlap). Equivalent to AAK48118 from Mycobacterium tuberculosis strain CDC1551 but shorter 18 aa. O69622 No conserved hypotheticals O69622 O69622 NC_000962.3 Mycobrowser_v5 CDS 4091233 4091517 . + 0 Rv3650 PE33 Function unknown PE family protein PE33 Rv3650, (MTCY15C10.02c), len: 94 aa. PE33, Short protein, member of the Mycobacterium tuberculosis PE family (see Brennan and Delogu, 2002), but without the repetitive gly-rich region, similar to the N-terminal part of many e.g. O53809|Rv0746|MTV041.20 PGRS-family protein (783 aa), FASTA scores: opt: 363, E(): 2.1e-15, (76.55% identity in 81 aa overlap). I6X7Z8 No PE/PPE Q7D550 I6X7Z8 I6X7Z8 NC_000962.3 Mycobrowser_v5 CDS 4093940 4094527 . + 0 Rv3653 PE_PGRS61 Function unknown PE-PGRS family-related protein PE_PGRS61 Rv3653, (MTV025.001B), len: 195 aa. PE_PGRS61, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), highly similar to the C-termini of members of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins, e.g. MTCY1A11_25, MTCY28_25, MTCY130_10, MTCY1A10_19, MTCY21B4_13, MTCI418B_6,MTCY28_34, MTV004_1, MTCY441_4; etc. Originally annotated as the second part of a PE-PGRS family protein (Rv3652/PE_PGRS60 being the first part). Start shortened since first submission (-50 aa). Q6MWV0 No PE/PPE Q6MWV0 NC_000962.3 Mycobrowser_v5 CDS 4094923 4095300 . - 0 Rv3655c Rv3655c Function unknown Conserved hypothetical protein Rv3655c, (MTV025.003c), len: 125 aa. Hypothetical protein, with similarity to Q9X917|SCH5.15c hypothetical 15.2 KDA protein from Streptomyces coelicolor (150 aa) FASTA scores: opt: 211, E(): 7.7e-07, (39.65% identity in 111 aa overlap). Equivalent to AAK48119 from Mycobacterium tuberculosis strain CDC1551 (99 aa) but longer 26 aa at the C-terminus. O69623 No conserved hypotheticals O69623 NC_000962.3 Mycobrowser_v5 CDS 4115157 4115894 . - 0 Rv3674c nth Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand (oxidized pyrimidines), 5' from the damaged site [catalytic activity: endonucleolytic cleavage near apurinic or apyrimidinic sites to products with 5'-phosphate]. Probable endonuclease III Nth (DNA-(apurinic or apyrimidinic site)lyase) (AP lyase) (AP endonuclease class I) (endodeoxyribonuclease (apurinic or apyrimidinic)) (deoxyribonuclease (apurinic or apyrimidinic)) Rv3674c, (MT3775, MTV025.022c), len: 245 aa. Probable nth, endonuclease III (see citation below), equivalent to Q9CB92|nth|ML2301 putative endonuclease III from Mycobacterium leprae (272 aa), FASTA scores: opt: 1363, E(): 3.6e-81, (89.4% identity in 226 aa overlap). Also similar to many e.g. Q9XA44|SCH17.03c from Streptomyces coelicolor (250 aa), FASTA scores: opt: 937, E(): 2.2e-55, (61.65% identity in 219 aa overlap); P46303|UVEN_MICLU from Micrococcus luteus (Micrococcus lysodeikticus) (279 aa), FASTA scores: opt: 899, E(): 8.1e-53, (58.45% identity in 248 aa overlap); P73715|END3_SYNY3|nth|SLR1822 from Synechocystis sp. strain PCC 6803 (219 aa), FASTA scores: opt: 684, E(): 1.7e-38, (52.2% identity in 203 aa overlap); P39788|END3_BACSU|nth|JOOB from Bacillus subtilis (219 aa), FASTA scores: opt: 552, E(): 1.2e-29, (43.3% identity in 194 aa overlap); etc. Equivalent to AAK48142 from Mycobacterium tuberculosis strain CDC1551 (262 aa) but shorter 17 aa. Contains PS00764 Endonuclease III iron-sulfur binding region signature, and PS01155 Endonuclease III family signature. Belongs to the nth/MUTY family. Cofactor: binds a 4FE-4S cluster which is not important for the catalytic activity, but which is probably involved in the proper positioning of the enzyme along the DNA strand (by similarity). N-terminus extended since first submission (previously 226 aa). P9WQ11 No information pathways P63540 P9WQ11 GO:0003677,GO:0003906,GO:0006284,GO:0004519,GO:0016798,GO:0005622,GO:0046872,GO:0051539 4.2.99.18 P9WQ11 NC_000962.3 Mycobrowser_v5 ncRNA 4100669 4100968 . + 0 MTB000078 MTS2823 Unknown Putative small regulatory RNA MTS2823, putative small regulatory RNA (See Arnvig et al., 2011), 5'-end mapped by RLM-RACE, 3'-end not mapped, ~300 bp and ~250 bp bands detected by Northern blot. No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 4098096 4099148 . - 0 Rv3660c Rv3660c Possibly plays a regulatory role in celular differentiation. Conserved hypothetical protein Rv3660c, (MTV025.008c), len: 350 aa. Conserved hypothetical protein, similar to O33612 protein concerned in inhibition of morphological differentiation in Streptomyces azureus from Streptomyces cyaneus (Streptomyces curacoi) (370 aa), FASTA scores: opt: 655, E(): 5.9e-31, (42.2% identity in 315 aa overlap); Q9X922|SCH5.20c putative septum site determining protein from Streptomyces coelicolor (396 aa), FASTA scores: opt: 592, E(): 2.9e-27, (43.25% identity in 275 aa overlap). And shows some similarity to AAK65513|CPAE2 probable CPAE2 PILUS assembly protein from Rhizobium meliloti (Sinorhizobium meliloti) plasmid pSymA (586 aa) FASTA scores: opt: 212, E(): 5.1e-05, (25.75% identity in 295 aa overlap); and several cell division inhibitors or septum site-determining proteins. Equivalent to AAK48124 from Mycobacterium tuberculosis strain CDC1551 (261 aa) but longer 89 aa. P9WKX7 No virulence, detoxification, adaptation P9WKX7 P9WKX7 NC_000962.3 Mycobrowser_v5 CDS 4101265 4102035 . - 0 Rv3662c Rv3662c Function unknown Conserved hypothetical protein Rv3662c, (MTV025.010c), len: 256 aa. Conserved hypothetical protein, equivalent to Q9CB99|ML2289 hypothetical protein from Mycobacterium leprae (256 aa) FASTA scores: opt: 1255, E(): 3.3e-69, (78.05% identity in 255 aa overlap). Also similar to Q9X924|SCH5.22c putative oxidoreductase from Streptomyces coelicolor (274 aa), FASTA scores: opt: 289, E(): 1.8e-10, (39.25% identity in 270 aa overlap). I6YCP7 No conserved hypotheticals I6YCP7 I6YCP7 NC_000962.3 Mycobrowser_v5 CDS 4129893 4130357 . - 0 Rv3688c Rv3688c Function unknown Conserved protein Rv3688c, (MTV025.036c), len: 154 aa. Conserved protein, similar to other bacterial hypothetical proteins e.g. Q9X934|SCH66.02c from Streptomyces coelicolor (154 aa), FASTA scores: opt: 425, E(): 3.4e-19, (46.1% identity in 154 aa overlap); Q9WZF4|TM0690 from Thermotoga maritima (149 aa), FASTA scores: opt: 326, E(): 3.4e-13, (40.4% identity in 151 aa overlap); Q9PHU3|CJ0573 from Campylobacter jejuni (147 aa), FASTA scores: opt:290 , E(): 5.1e-11, (36.4% identity in 151 aa overlap); etc. Also some similarity to upstream O69654|Rv3686c|MTV025.034c conserved hypothetical protein from Mycobacterium tuberculosis. I6X831 No conserved hypotheticals O69656 I6X831 GO:0006412,GO:0016884 I6X831 NC_000962.3 Mycobrowser_v5 CDS 4197628 4198137 . - 0 Rv3749c Rv3749c Function unknown Conserved hypothetical protein Rv3749c, (MTV025.097c), len: 169 aa. Hypothetical protein, showing some similarity with O85864 hypothetical 21.4 KDA protein from Sphingomonas aromaticivorans plasmid pNL1 (196 aa), FASTA scores: opt: 148, E(): 0.011, (32.7% identity in 104 aa overlap); Q9LCU6 hypothetical 21.2 KDA protein from Arthrobacter sp. TM1 (192 aa), FASTA scores: opt: 125, E(): 0.35, (31.5% identity in 92 aa overlap); Q9L631|SPCB myo-inositol-2-dehydrogenase from Streptomyces spectabilis (374 aa); Q9WJP8|PRE-S1 PRE-S1 protein (fragment) from Hepatitis B virus (88 aa); etc. Contains PS00092 N-6 Adenine-specific DNA methylases signature. Predicted to be an outer membrane protein (See Song et al., 2008). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L0TGF0 No conserved hypotheticals L0TGF0 NC_000962.3 Mycobrowser_v5 CDS 4103675 4104475 . - 0 Rv3664c dppC Involved in active transport of dipeptide across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable dipeptide-transport integral membrane protein ABC transporter DppC Rv3664c, (MTV025.012c), len: 266 aa. Probable dppC, dipeptide-transport integral membrane protein ABC-transporter (see Braibant et al., 2000), similar to many peptide permeases e.g. Q9F351|SC9E12.04 putative peptide transport system integral membrane from Streptomyces coelicolor (305 aa), FASTA scores: opt: 901, E(): 1.1e-47, (51.15% identity in 262 aa overlap); Q9KFX1|APPC|BH0349 oligopeptide ABC transporter (permease) from Bacillus halodurans (305 aa), FASTA scores: opt: 652, E(): 1.5e-32, (35.55% identity in 270 aa overlap); P94312|DPPC_BACFI dipeptide transport system permease protein from Bacillus firmus (304 aa), FASTA scores: opt: 642, E(): 5.9e-32, (35.75% identity in 263 aa overlap); P24139|OPPC_BACSU|SPO0KC oligopeptide transport system permease protein from Bacillus subtilis (305 aa), FASTA scores: opt: 637, E(): 1.2e-31, (37.4% identity in 262 aa overlap); P26904|DPPC_BACSU|DCIAC dipeptide transport system permease protein from Bacillus subtilis (320 aa), FASTA scores: opt: 621, E(): 1.2e-30, (39.9% identity in 263 aa overlap); etc. Has similarity with integral membrane components of other binding-protein-dependent transport systems. Belongs to the OPPBC subfamily. L0TEV4 No cell wall and cell processes O69632 L0TEV4 GO:0005886,GO:0006810,GO:0005215,GO:0016021 L0TEV4 NC_000962.3 Mycobrowser_v5 CDS 4114474 4115157 . - 0 Rv3673c Rv3673c Function not known: possibly acts on thioredoxin. Possible membrane-anchored thioredoxin-like protein (thiol-disulfide interchange related protein) Rv3673c, (MTV025.021c), len: 227 aa. Possible membrane protein, thioredoxin-like protein (thiol-disulfide interchange protein), equivalent to Q9CB93|ML2300 putative membrane protein from Mycobacterium leprae (215 aa), FASTA scores: opt: 978, E(): 2.5e-52, (71.15% identity in 215 aa overlap). Some similarity with thioredoxin-related proteins e.g. P35160|RESA_BACSU RESA protein from Bacillus subtilis (181 aa), FASTA scores: opt: 212, E(): 5.7e-06, (30.55% identity in 108 aa overlap); Q9RXW6|DR0189 thiol:disulfide interchange protein from Deinococcus radiodurans (185 aa) FASTA scores: opt: 206, E(): 1.3e-05, (33.8% identity in 139 aa overlap); Q9I505|PA0953 probable thioredoxin from Pseudomonas aeruginosa (154 aa), FASTA scores: opt: 180, E(): 0.00044, (34.85% identity in 109 aa overlap); Q9KCP7|BH1522 thioredoxin (thiol:disulfide interchange protein) from Bacillus halodurans (177 aa), FASTA scores: opt: 178, E(): 0.00064, (31.75% identity in 107 aa overlap); P43221|TLPA_BRAJA thiol:disulfide interchange protein (cytochrome C biogenesis protein) from Bradyrhizobium japonicum (221 aa), FASTA scores: opt: 189, E(): 0.00017, (26.85% identity in 227 aa overlap); etc. Also similar to O06392|Rv0526|MTCY25D10.05 hypothetical 23.2 KDA protein from Mycobacterium tuberculosis (216 aa) FASTA scores: opt: 160, E(): 0.0093, (27.45% identity in 142 aa overlap). Contains PS00194 Thioredoxin family active site. Possibly belongs to the thioredoxin family. I6YGW6 No intermediary metabolism and respiration O69641 I6YGW6 GO:0055114,GO:0016491,GO:0045454 1.-.-.- I6YGW6 NC_000962.3 Mycobrowser_v5 CDS 4104531 4105457 . - 0 Rv3665c dppB Involved in active transport of dipeptide across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable dipeptide-transport integral membrane protein ABC transporter DppB Rv3665c, (MTV025.013c), len: 308 aa. Probable dppB, dipeptide-transport integral membrane protein ABC-transporter (see citation below), similar to many peptide permeases e.g. Q9F352|SC9E12.03 putative peptide transport system integral membrane protein from Streptomyces coelicolor (307 aa), FASTA scores: opt: 1145, E(): 1.8e-61, (57.65% identity in 307 aa overlap); Q53191|Y4TP_RHISN probable peptide ABC transporter permease protein Rhizobium sp. strain NGR234 (313 aa), FASTA scores: opt: 653, E(): 5.2e-32, (31.2% identity in 314 aa overlap); P24138|OPPB_BACSU oligopeptide transport system permease from Bacillus subtilis (311 aa), FASTA scores: opt: 643, E(): 2.1e-31, (33.45% identity in 305 aa overlap); etc. Belongs to the OPPBC subfamily. I6YGV9 No cell wall and cell processes O69633 I6YGV9 GO:0005886,GO:0006810,GO:0005215,GO:0016021 I6YGV9 NC_000962.3 Mycobrowser_v5 CDS 4109783 4110481 . - 0 Rv3668c Rv3668c Function unknown; hydrolyses peptides and/or proteins. Possible protease Rv3668c, (MTV025.016c), len: 232 aa. Possible protease (and more specifically a putative alkaline serine protease, equivalent to Q9CB98|ML2295 hypothetical protein from Mycobacterium leprae (234 aa), FASTA scores: opt: 1249, E(): 7.4e-66, (77.5% identity in 231 aa overlap). Also similar at C-terminal end with many proteases e.g. O86984 alkaline serine protease precursor from Thermomonospora fusca (368 aa), FASTA scores: opt: 190, E(): 0.00056, (28.9% identity in 173 aa overlap); Q55353|SAPII alkaline serine protease II from Streptomyces sp (382 aa), FASTA scores: opt: 160, E(): 0.032, (27.15% identity in 199 aa overlap); O54109|SC10A5.18 putative secreted protease from Streptomyces coelicolor (411 aa), FASTA scores: opt: 155, E(): 0.066, (26.4% identity in 163 aa overlap); Q54392|SAL|SCI11.35C serine protease SAL precursor (300 aa), FASTA scores: opt: 153, E(): 0.068, (28.1% identity in 185 aa overlap); P00778|PRLA_LYSEN|alpha-LP alpha-LYTIC protease precursor (397 aa), FASTA scores: opt: 154, E(): 0.074, (26.75% identity in 172 aa overlap); etc. Also similar with Q50618|YI15_MYCTU|Rv1815|MT1863|MTCY1A11.28c hypothetical 22.8 KDA protein from Mycobacterium tuberculosis (221 aa), FASTA scores: opt: 134, E(): 0.69, (30.95% identity in 181 aa overlap). Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). I6YGW2 No intermediary metabolism and respiration I6YGW2 GO:0008233 3.4.-.- I6YGW2 NC_000962.3 Mycobrowser_v5 CDS 4110827 4111345 . + 0 Rv3669 Rv3669 Unknown Probable conserved transmembrane protein Rv3669, (MTV025.017), len: 172 aa. Probable conserved transmembrane protein, equivalent to Q9CB97|ML2296 putative membrane protein from Mycobacterium leprae (181 aa), FASTA scores: opt: 863, E(): 1.4e-47, (77.35% identity in 181 aa overlap). Also similar to two putative integral membrane transport proteins from Streptomyces coelicolor; Q9X930|SCH5.28 (162 aa) FASTA scores: opt: 265, E(): 6.3e-10, (37.4% identity in 155 aa overlap); and Q9X9W1|SCI7.29c (165 aa), FASTA scores: opt: 194, E(): 1.9e-05, (30.6% identity in 134 aa overlap). Contains two hydrophobic stretches in centre. O69637 No cell wall and cell processes O69637 O69637 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 4116478 4117152 . + 0 Rv3676 crp Involved in transcriptional mechanism. Transcriptional regulatory protein Crp (Crp/Fnr-family) Rv3676, (MTV025.024), len: 224 aa. Crp, transcriptional regulator belonging to crp/fnr family, identical to Q9CB91|ML2302 putative Crp/Fnr-family transcriptional regulator from Mycobacterium leprae (224 aa), FASTA scores: opt: 1408, E(): 8.8e-81, (95.95% identity in 224 aa overlap). Also highly similar to transcriptional regulators AAK58838 from Corynebacterium glutamicum (Brevibacterium flavum) (227 aa), FASTA scores: opt: 1178, E(): 1.9e-66, (79.9% identity in 224 aa overlap); and Q9XA42|SCH17.05 from Streptomyces coelicolor (224 aa), FASTA scores: opt: 869, E(): 3.4e-47, (54.45% identity in 224 aa overlap); and similar to others e.g. Q9RRX0|DR2362 from Deinococcus radiodurans (231 aa) FASTA scores: opt: 344, E(): 1.8e-14, (30.8% identity in 211 aa overlap); P29281|CRP_HAEIN from Haemophilus influenzae (224 aa), FASTA scores: opt: 330, E(): 1.3e-13, (32.25% identity in 189 aa overlap); P03020|CRP_ECOLI|cap|CSM|B3357 from Escherichia coli strain K12 and Shigella flexneri (210 aa), FASTA scores: opt: 323, E(): 3.5e-13, (32.25% identity in 189 aa overlap); etc. Contains helix-turn-helix motif at aa 175-196 (Score 1990, +5.96 SD). Belongs to the Crp/Fnr family of transcriptional regulators. Binds cAMP. P9WMH3 No regulatory proteins 3D0S,3H3U,3I54,3I59,3MZH O69644 P9WMH3 GO:0006355,GO:0006350,GO:0003700,GO:0005622 P9WMH3 NC_000962.3 Mycobrowser_v5 CDS 4113521 4114342 . - 0 Rv3672c Rv3672c Function unknown Conserved hypothetical protein Rv3672c, (MTV025.020c), len: 273 aa. Conserved hypothetical protein, equivalent to Q9CB94|ML2299 hypothetical protein from Mycobacterium leprae (266 aa) FASTA scores: opt: 1358, E(): 5.2e-75, (76.4% identity in 267 aa overlap). Also similar to others (generally in C-terminal end) e.g. Q9XA45|SCH17.02c hypothetical 26.5 KDA protein from Streptomyces coelicolor (247 aa) FASTA scores: opt: 524, E(): 1.3e-24, (42.65% identity in 251 aa overlap); Q9AB27|CC0407 mutt/NUDIX family protein from Caulobacter crescentus (216 aa), FASTA scores: opt: 285, E(): 3.2e-10, (36.2% identity in 174 aa overlap); BAB49788|MLL2727|Q98HS8 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (204 aa), FASTA scores: opt: 278, E(): 8.1e-10, (31.45% identity in 151 aa overlap); P43337|YEAB_ECOLI|B1813 hypothetical 21.4 KDA protein from Escherichia coli strain K12 (192 aa) FASTA scores: opt: 252, E(): 2.9e-08, (35.9% identity in 170 aa overlap); etc. Contains PS01293 Uncharacterized protein family UPF0036 signature, LLT. I6XHX8 No conserved hypotheticals O69640 I6XHX8 GO:0000287,GO:0030145,GO:0009132,GO:0016818 I6XHX8 NC_000962.3 Mycobrowser_v5 CDS 4117258 4118052 . - 0 Rv3677c Rv3677c Function unknown; probably involved in cellular metabolism. Possible hydrolase Rv3677c, (MTV025.025c), len: 264 aa. Possible hydrolase, equivalent to Q9CB90|ML2303 putative hydrolase from Mycobacterium leprae (262 aa) FASTA scores: opt: 1400, E(): 8.5e-81, (82.05% identity in 262 aa overlap). Also similar to other hydrolases and hypothetical proteins e.g. Q9XA41|SCH17.06c putative hydrolase from Streptomyces coelicolor (256 aa) FASTA scores: opt: 609, E(): 3.9e-31, (54.65% identity in 247 aa overlap); Q9A9Q1|CC0923 metallo-beta-lactamase family protein from Caulobacter crescentus (297 aa), FASTA scores: opt: 306, E(): 4.7e-12, (35.45% identity in 268 aa overlap); Q9Y392 CGI-83 protein from Homo sapiens (Human) (288 aa), FASTA scores: opt: 281, E(): 1.7e-10, (33.2% identity in 259 aa overlap); Q9F7R6 predicted metallobeta lactamase fold protein from uncultured proteobacterium EBAC31A08 (265 aa), FASTA scores: opt: 257, E(): 5.1e-09, (32.55% identity in 252 aa overlap); Q9PBI4|XF2160 hydroxyacylglutathione hydrolase from Xylella fastidiosa (258 aa), FASTA scores: opt: 232, E(): 1.9e-07, (30.3% identity in 165 aa overlap); etc. Recombinant protein has beta lactamase activity (See Nampoothiri et al., 2008). I6XHY3 No intermediary metabolism and respiration I6XHY3 GO:0016787 3.-.-.- I6XHY3 NC_000962.3 Mycobrowser_v5 CDS 4118059 4118514 . - 0 Rv3678c Rv3678c Function unknown Conserved protein Rv3678c, (MTV025.026c), len: 151 aa. Conserved protein, equivalent, but shorter 23 aa, to Q9CB89|ML2304 hypothetical protein from Mycobacterium leprae (174 aa), FASTA scores: opt: 746, E(): 2.1e-40, (78.15% identity in 151 aa overlap). Also highly similar to many hypothetical proteins or transcription regulators e.g. Q9XA38|SCH17.09c from Streptomyces coelicolor (155 aa), FASTA scores: opt: 637, E(): 1.5e-33, (69.1% identity in 152 aa overlap); BAB48205|MLR0658 from Rhizobium loti (Mesorhizobium loti) (154 aa), FASTA scores: opt: 500, E(): 6.8e-25, (55.35% identity in 150 aa overlap); BAB50615|MLR3802 transcription regulator from Rhizobium loti (Mesorhizobium loti) (153 aa), FASTA scores: opt: 425,E(): 3.8e-20, (44.35% identity in 151 aa overlap); Q9U0W7|L7276.02 from Leishmania major (163 aa) FASTA scores: opt: 404, E(): 8.5e-19, (47.7% identity in 151 aa overlap); Q9UZA3|PAB0825 putative translation initiation inhibitor from Pyrococcus abyssi (127 aa), FASTA scores: opt: 108, E(): 3.7, (30.75% identity in 130 aa overlap); etc. Contains PS00044 Bacterial regulatory proteins, lysR family signature. I6YGW9 No conserved hypotheticals O69646 I6YGW9 I6YGW9 NC_000962.3 Mycobrowser_v5 CDS 4119795 4120955 . + 0 Rv3680 Rv3680 Anion-transporting ATPase; supposedly catalyzes the extrusion of undetermined anions [catalytic activity: ATP + H(2)O + undetermined anion(in) = ADP + phosphate + undetermined anion(out)]. Probable anion transporter ATPase Rv3680, (MTV025.028), len: 386 aa. Probable anion transporting ATPase, equivalent to Q9CB87|ML2306 probable anion transporter protein from Mycobacterium leprae (381 aa), FASTA scores: opt: 2131, E(): 6.5e-120, (88.1% identity in 370 aa overlap). Also highly similar, but shorter 29 aa, to Q9XA35|SCH17.12 putative ion-transporting ATPase from Streptomyces coelicolor (481 aa), FASTA scores: opt: 1190, E(): 1.1e-63, (51.25% identity in 441 aa overlap); and similar to many anion transporting ATPases e.g. Q9UZA6|PAB1555 anion transporting ATPase from Pyrococcus abyssi (330 aa) FASTA scores: opt: 242, E(): 3e-07, (24.6% identity in 297 aa overlap); Q9P7F8|SPAC1142.06 putative arsenite-translocating from Schizosaccharomyces pombe (Fission yeast) (329 aa), FASTA scores: opt: 239, E(): 4.5e-07, (27.9% identity in 197 aa overlap); Q9HS79|ARSA1|VNG0365G arsenical pump-driving ATPase from Halobacterium sp. strain NRC-1 (347 aa), FASTA scores: opt: 238, E(): 5.4e-07, (29.35% identity in 358 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). I6Y498 No cell wall and cell processes I6Y498 GO:0016787 3.6.1.- I6Y498 NC_000962.3 Mycobrowser_v5 CDS 4121198 4121554 . - 0 Rv3681c whiB4 Involved in a transcriptional mechanism. Probable transcriptional regulatory protein WhiB-like WhiB4 Rv3681c, (MTV025.029c), len: 118 aa. Probable whiB4 (alternate gene name: whmA), WhiB-like regulatory protein (see Hutter & Dick 1999), similar to WhiB paralogue of Streptomyces coelicolor, wblE gene product (85 aa). Equivalent to ML2307 hypothetical protein from Mycobacterium leprae (116 aa). Also highly similar to Q9S2B9|SCH17.13c putative regulatory protein from Streptomyces coelicolor (112 aa), FASTA scores: opt: 392, E(): 1e-20, (67.95% identity in 78 aa overlap); Q9X951|WBLA hypothetical 14.3 KDA protein from Streptomyces coelicolor (129 aa), FASTA scores: opt: 392, E(): 1.1e-20, (67.95% identity in 78 aa overlap); Q9ACZ0|SCP1.161c putative regulatory protein from Streptomyces coelicolor (268 aa), FASTA scores: opt: 273, E(): 4.4e-12, (50.0% identity in 78 aa overlap); Q06387|WHIB-STV from Streptomyces griseocarneus (87 aa) FASTA scores: opt: 231, E(): 1.5e-09, (43.85% identity in 73 aa overlap); etc. Also similar to several putative regulator proteins from Mycobacterium tuberculosis e.g. MTCY7D11_7; MTCY78_13; MTCY10H4_23; MTCY1A6_6; and U00016_29 from Mycobacterium leprae. N-terminus shortened since first submission. P9WF39 No regulatory proteins O69649 P9WF39 P9WF39 NC_000962.3 Mycobrowser_v5 CDS 4121916 4124348 . + 0 Rv3682 ponA2 Involved in peptidoglycan synthesis (at the final stages), cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits). Supposedly involved in stationary-phase survival. Probable bifunctional membrane-associated penicillin-binding protein 1A/1B PonA2 (murein polymerase) [includes: penicillin-insensitive transglycosylase (peptidoglycan TGASE) + penicillin-sensitive transpeptidase (DD-transpeptidase)] Rv3682, (MTV025.030), len: 810 aa. Probable ponA2, penicillin-binding protein (class A), bienzymatic membrane-associated protein with transglycosylase and transpeptidase activities. Almost identical to Q9CB85|PON1|ML2308 penicillin binding protein (class A) from Mycobacterium leprae (803 aa) FASTA scores: opt: 4743, E(): 3.3e-217, (87.7% identity in 806 aa overlap); or P72351|PON1|PBP1 high-molecular-mass class a penicillin binding protein from Mycobacterium leprae Cosmid B577 (821 aa), FASTA scores: opt: 4547, E(): 6.3e-208, (88.05% identity in 769 aa overlap) (see Basu et al., 1996). Also equivalent to a predicted homologous protein from Mycobacterium smegmatis. Also similar to others e.g. Q9XA34|SCH17.14 from Streptomyces coelicolor (428 aa; fragment), FASTA scores: opt: 727, E(): 2.3e-27, (36.55% identity in 413 aa overlap); Q9F9V7|PONA from Mycobacterium smegmatis (715 aa), FASTA scores: opt: 446, E(): 6.6e-14, (27.65% identity in 771 aa overlap) (see Billman-Jacobe et al., 1999); Q9CCY4|PONA|ML2688 from Mycobacterium leprae (708 aa), FASTA scores: opt: 413, E(): 2.4e-12, (26.8% identity in 660 aa overlap); Q9X6W0|PONB|MRCB|PA4700 from Pseudomonas aeruginosa (774 aa), FASTA scores: opt: 398, E(): 1.3e-11, (27.2% identity in 666 aa overlap); P45345|PBPB_HAEIN|MRCB|PONB|HI1725 (781 aa), FASTA scores: opt: 380, E(): 9.4e-11, (28.6% identity in 601 aa overlap); etc. Also similar to P71707|PONA1|Rv0050|MTCY21.13 probable bifunctional penicillin-binding protein 1A/1B (PBP1) from Mycobacterium tuberculosis (678 aa) FASTA scores: opt: 372, E(): 2e-10, (28.35% identity in 769 aa overlap). Seems to belong to the transglycosylase family in the N-terminal section, and to the transpeptidase family in the C-terminal section. I6YGX2 No cell wall and cell processes Q7D529 I6YGX2 GO:0016787,GO:0008658,GO:0009252,GO:0009274,GO:0008360,GO:0016757,GO:0007047 I6YGX2 NC_000962.3 Mycobrowser_v5 CDS 4139805 4140242 . - 0 Rv3697c vapC48 Unknown Possible toxin VapC48. Contains PIN domain. Rv3697c, (MTV025.045c), len: 145 aa. Possible vapC48, toxin, part of toxin-antitoxin (TA) operon with Rv3697A, contains PIN domain, see Arcus et al. 2005. Similar to many others in Mycobacterium tuberculosis e.g. Q10800|YS72_MYCTU|Rv2872|MT2939|MTCY274.03 (147 aa) FASTA scores: opt: 223, E(): 7.3e-08, (32.6% identity in 141 aa overlap); O53501|Rv2103c|MTV020.03 (144 aa), FASTA scores: opt: 215, E(): 2.4e-07, (31.4% identity in 137 aa overlap); O53812|Rv0749|MTV041.23 (142 aa), FASTA scores: opt: 192, E(): 7.6e-06, (31.25% identity in 144 aa overlap); etc. P9WF47 No virulence, detoxification, adaptation O69665 P9WF47 P9WF47 NC_000962.3 Mycobrowser_v5 CDS 4125439 4126479 . + 0 Rv3684 Rv3684 Function unknown; probably involved in cellular metabolism. Probable lyase Rv3684, (MTV025.032), len: 346 aa. Probable lyase , and more specifically a cysteine synthase, highly similar to many lyases e.g. Q9K3N2|SCG20A.08c putative lyase from Streptomyces coelicolor (374 aa), FASTA scores: opt: 1469, E(): 3.7e-85, (63.35% identity in 341 aa overlap) (shorter 31 aa at N-terminus); Q9KT44|VC1061 cysteine synthase/ cystathionine beta-synthase family protein from Vibrio cholerae (355 aa), FASTA scores: opt: 1366, E(): 1.1e-78, (63.25% identity in 321 aa overlap); Q9I4R3|PA1061 hypothetical protein from Pseudomonas aeruginosa (365 aa), FASTA scores: opt: 1311, E(): 3.2e-75, (59.8% identity in 341 aa overlap); Q9PH18|XF0128 cysteine synthase from Xylella fastidiosa (390 aa), FASTA scores: opt: 1288, E(): 9.5e-74, (58.55% identity in 333 aa overlap) (shorter 34 aa at N-terminus); P55708|Y4XP_RHISN putative cysteine synthase from Rhizobium sp. strain NGR234 plasmid sym pNGR234a (336 aa), FASTA scores: opt: 376, E(): 2.1e-16, (29.2% identity in 315 aa overlap); etc. Equivalent to AAK48153 from Mycobacterium tuberculosis strain CDC1551 (368 aa) but shorter 22 aa. O69652 No intermediary metabolism and respiration O69652 O69652 GO:0008152,GO:0030170,GO:0016829 4.-.-.- O69652 NC_000962.3 Mycobrowser_v5 CDS 4127295 4128725 . - 0 Rv3685c cyp137 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Probable cytochrome P450 137 Cyp137 Rv3685c, (MTV025.033c), len: 476 aa. Probable cyp137, cytochrome P-450, similar to many e.g. Q9VXY0|C4S3_DROME|CYP4S3|CG9081 from Drosophila melanogaster (Fruit fly) (495 aa), FASTA scores: opt: 376, E(): 1.2e-15, (28.35% identity in 413 aa overlap); Q59163|CYP110A2 from Anabaena variabilis (459 aa) FASTA scores: opt: 320, E(): 3.1e-12, (31.4% identity in 411 aa overlap); O23051|C883_ARATH from Arabidopsis thaliana (Mouse-ear cress) (490 aa), FASTA scores: opt: 313, E(): 8.8e-12, (28.25% identity in 425 aa overlap); etc. Also similar to many from Mycobacterium tuberculosis e.g. O53765|C13B_MYCTU|CYP135B1|Rv0568|MT0594|MTV039.06 (472 aa), FASTA scores: opt: 920, E(): 4.6e-49, (36.25% identity in 447 aa overlap); P96813|C138_MYCTU|CYP138|Rv0136|MT0144|MTCI5.10 (441 aa) FASTA scores: opt: 886, E(): 5.3e-47, (35.5% identity in 445 aa overlap); etc. Belongs to the cytochrome P450 family. P9WPM5 No intermediary metabolism and respiration O69653 P9WPM5 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPM5 NC_000962.3 Mycobrowser_v5 CDS 4128751 4129083 . - 0 Rv3686c Rv3686c Function unknown Conserved hypothetical protein Rv3686c, (MTV025.034c), len: 110 aa. Hypothetical protein, similar to P96893|Rv3288c|MTCY71.28c hypothetical 15.2 KDA protein from Mycobacterium tuberculosis (and Mycobacterium bovis) (137 aa) FASTA scores: opt: 106, E(): 5.6, (29.1% identity in 79 aa overlap); and a few hypothetical proteins e.g. Q9GUV6|L2259.2 from Leishmania major (360 aa) FASTA scores: opt: 118, E(): 2.1, (28.7% identity in 101 aa overlap). Equivalent to AAK48155 from Mycobacterium tuberculosis strain CDC1551 (166 aa) but shorter 56 aa. O69654 No conserved hypotheticals O69654 NC_000962.3 Mycobrowser_v5 CDS 4133516 4134592 . + 0 Rv3692 moxR2 Involved in transcriptional mechanism; regulates methanol dehydrogenase. Probable methanol dehydrogenase transcriptional regulatory protein MoxR2 Rv3692, (MTV025.040), len: 358 aa. Probable moxR2, methanol dehydrogenase regulatory protein, highly similar (generally longer at N-terminus) to Q9KYW3|SCE33.20 putative regulatory protein from Streptomyces coelicolor (329 aa), FASTA scores: opt: 1523, E(): 4.2e-74, (70.9% identity in 330 aa overlap); Q9Z538|SC9B2.21c putative regulatory protein from Streptomyces coelicolor (332 aa) FASTA scores: opt: 1008, E(): 1.1e-46, (50.8% identity in 313 aa overlap); Q9UZ67|MOXR-3|PAB0848 methanol dehydrogenase regulatory protein from Pyrococcus abyssi (314 aa), FASTA scores: opt: 989, E(): 1.1e-45, (50.65% identity in 302 aa overlap); Q9AAN1|CC0566 MOXR protein from Caulobacter crescentus (323 aa), FASTA scores: opt: 988, E(): 1.3e-45, (52.3% identity in 306 aa overlap); etc. Also similar to O53170|MTV007.26|MOXR|Rv1479 from Mycobacterium tuberculosis (377 aa); and O07392|AF002133_6|MOXR from Mycobacterium avium (309 aa). Also high similarity with several hypothetical bacterial proteins. I6YGX9 No regulatory proteins O69660 I6YGX9 GO:0016887,GO:0005524 I6YGX9 NC_000962.3 Mycobrowser_v5 CDS 4134726 4136048 . + 0 Rv3693 Rv3693 Unknown Possible conserved membrane protein Rv3693, (MTV025.041), len: 440 aa (alternative start at 41910). Possible conserved membrane protein, similar to Q9KYW2|SCE33.21 putative lipoprotein from Streptomyces coelicolor (436 aa), FASTA scores: opt: 875, E(): 3.3e-46, (56.25% identity in 448 aa overlap); Q9AAN0|CC0567 hypothetical protein from Caulobacter crescentus (437 aa), FASTA scores: opt: 355, E(): 2.3e-14, (30.9% identity in 450 aa overlap); P73233|SLR2013 hypothetical 48.5 KDA protein from Synechocystis sp. strain PCC 6803 (435 aa), FASTA scores: opt: 340, E(): 1.9e-13, (29.7% identity in 438 aa overlap); etc. Equivalent to AAK48162 from Mycobacterium tuberculosis strain CDC1551 (475 aa) but shorter 35 aa. Also similar to other hypothetical proteins from Mycobacterium tuberculosis; MTV014_7; MTV007_27; and MTCY71_36 M. Predicted to be an outer membrane protein (See Song et al., 2008). O69661 No cell wall and cell processes O69661 O69661 O69661 NC_000962.3 Mycobrowser_v5 CDS 4136122 4137114 . - 0 Rv3694c Rv3694c Unknown Possible conserved transmembrane protein Rv3694c, (MTV025.042c), len: 330 aa. Possible conserved transmembrane protein, highly similar to Q9KZM4|SCE34.01c putative integral membrane protein from Streptomyces coelicolor (335 aa), FASTA scores: opt: 1113, E(): 2.5e-60, (51.5% identity in 334 aa overlap); and similar to Q9KEW6|BH0733 hypothetical protein from Bacillus halodurans (355 aa), FASTA scores: opt: 381, E(): 6.1e-16, (24.15% identity in 331 aa overlap); Q9AAM9|CC0568 hypothetical protein from Caulobacter crescentus (332 aa), FASTA scores: opt: 352, E(): 3.3e-14, (30.3% identity in 310 aa overlap); P74166|SLR1478 hypothetical 35.4 KDA protein from Synechocystis sp. strain PCC 6803 (317 aa), FASTA scores: opt: 330, E(): 6.8e-13, (25.65% identity in 308 aa overlap); etc. C-terminal end shows similarity to O29631|AF0624|AE001061_10 conserved hypothetical protein (putative nifU protein) from Archaeoglobus fulgidus (185 aa), FASTA scores: opt: 154, E(): 0.021, (29.0% identity in 131 aa overlap). Equivalent to AAK48163 from Mycobacterium tuberculosis strain CDC1551 (395 aa) but shorter 65 aa. Also some similarity to MTCY428_20 hypothetical 43.7 KDA protein from Mycobacterium tuberculosis. O69662 No cell wall and cell processes O69662 O69662 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 4143951 4144916 . - 0 Rv3701c Rv3701c Function unknown Conserved hypothetical protein Rv3701c, (MTV025.049c), len: 321 aa. Conserved hypothetical protein, highly similar to other hypothetical proteins e.g. Q9RCZ8|SCM1.46 from Streptomyces coelicolor (251 aa), FASTA scores: opt: 897, E(): 1.1e-50, (59.9% identity in 242 aa overlap); P73759|SLR0865 from Synechocystis sp. strain PCC 6803 (337 aa), FASTA scores: opt: 779, E(): 5.7e-43, (40.35% identity in 327 aa overlap); Q9GWA1|LM12.997 from Leishmania major (383 aa) FASTA scores: opt: 616, E(): 2.1e-32, (39.05% identity in 297 aa overlap); etc. P9WN47 No conserved hypotheticals O69669 P9WN47 P9WN47 NC_000962.3 Mycobrowser_v5 CDS 4140493 4142022 . + 0 Rv3698 Rv3698 Function unknown Conserved protein Rv3698, (MTV025.046), len: 509 aa. Conserved protein, highly similar to Q9AK89|SC10A9.15c conserved hypothetical protein from Streptomyces coelicolor (505 aa), FASTA scores: opt: 1720, E(): 9e-103, (53.65% identity in 494 aa overlap). N-terminal end highly similar to CAC42136|SCBAC25F8.01 conserved hypothetical protein (fragment) from Streptomyces coelicolor (291 aa), FASTA scores: opt: 1078, E(): 8.7e-62, (52.6% identity in 291 aa overlap); and C-terminus highly similar to CAC44687|SCBAC17A6.42c (235 aa), FASTA scores: opt: 911, E(): 3.8e-51, (57.25% identity in 234 aa overlap). I6YCS6 No conserved hypotheticals I6YCS6 I6YCS6 NC_000962.3 Mycobrowser_v5 CDS 4142044 4142745 . + 0 Rv3699 Rv3699 Function unknown Conserved protein Rv3699, (MTV025.047), len: 233 aa. Conserved protein, showing similarity with hypothetical proteins e.g. Q9P3V6|SPAC1348.04 (alias Q9P3E7|SPAC750.03c or Q9P7U5|SPAC977.03) from Schizosaccharomyces pombe (Fission yeast) (145 aa), FASTA scores: opt: 188, E(): 7.5e-05, (31.65% identity in 120 aa overlap); and Q9KB70|BH2058 from Bacillus halodurans (241 aa) FASTA scores: opt: 185, E(): 0.00018, (27.8% identity in 162 aa overlap); Q9XA90|SCF43A.25c putative methyltransferase from Streptomyces coelicolor (215 aa), FASTA scores: opt: 166, E(): 0.0025, (29.95% identity in 147 aa overlap); etc. Also highly similar to O06426|Rv0560c|MTCY25D10.39c hypothetical 25.9 KDA protein from Mycobacterium tuberculosis (241 aa), FASTA scores: opt: 690, E(): 6.5e-36, (53.4% identity in 234 aa overlap); and similar to other hypothetical proteins from Mycobacterium tuberculosis e.g. P71805|Rv1377c|MTCY02B12.11c (212 aa) FASTA scores: opt: 378, E(): 1.5e-16, (35.4% identity in 192 aa overlap); P71972|Rv2675c|MTCY441.44c (250 aa) FASTA scores: opt: 297, E(): 2e-11, (31.1% identity in 193 aa overlap); etc. O69667 No conserved hypotheticals O69667 O69667 GO:0008152,GO:0008119,GO:0005737 O69667 NC_000962.3 Mycobrowser_v5 CDS 4169606 4170169 . + 0 Rv3724B cut5b Hydrolysis of cutin (a polyester that forms the structure of plant cuticle). Probable cutinase [second part] Cut5b Rv3724B, (MTV025.072), len: 187 aa. Probable cut5b, truncated cutinase, similar to C-terminal end of others e.g. Q9XB09|RVD2-RV1758 protein (fragment) from Mycobacterium bovis BCG (143 aa) FASTA scores: opt: 335, E(): 3.4e-12, (53.25% identity in 92 aa overlap); Q9KK87 serine esterase cutinase from Mycobacterium avium (220 aa), FASTA scores: opt: 251, E(): 2.5e-07, (44.05% identity in 168 aa overlap). Also similar to proteins from Mycobacterium tuberculosis e.g. O06793|Rv1758|MTCY28.24 hypothetical 17.9 KDA protein (174 aa), FASTA scores: opt: 641, E(): 2.5e-29, (57.25% identity in 166 aa overlap); O06319|Rv3452|MTCY13E12.05 hypothetical 23.1 KDA protein (226 aa), FASTA scores: opt: 385, E(): 7.5e-15, (46.65% identity in 165 aa overlap); O06318|CUT3_MYCTU|Rv3451|MT3557|MTCY13E12.04 probable cutinase precursor (247 aa), FASTA scores: opt: 307, E(): 1.9e-10, (40.7% identity in 167 aa overlap); Q10837|CUT1_MYCTU|Rv1984c|MT2037|MTCY39.35 probable cutinase precursor (217 aa), FASTA scores: opt: 261, E(): 6.7e-08, (50.9% identity in 169 aa overlap); etc; and U00015_11 from Mycobacterium lepra. 5'-end of gene is Rv3724A|CUT5A; frameshifting may occur near position 4169668. Q79FA4 No cell wall and cell processes Q79FA4 Q79FA4 GO:0008152,GO:0016787 3.1.1.- Q79FA4 NC_000962.3 Mycobrowser_v5 CDS 4148318 4148962 . - 0 Rv3705c Rv3705c Function unknown Conserved protein Rv3705c, (MTV025.053c), len: 214 aa. Conserved protein, equivalent to Q9CB80|ML2320 hypothetical protein from Mycobacterium leprae (215 aa) FASTA scores: opt: 1145, E(): 5.9e-68, (79.45% identity in 214 aa overlap). Some similarity to the C-terminal end of Q11053|PKNH_MYCTU|Rv1266c|MT1304|MTCY50.16 probable serine/threonine-protein from Mycobacterium tuberculosis (626 aa), FASTA scores: opt: 175, E(): 0.0005, (24.9% identity in 201 aa overlap); and to the N-terminal end of P23903|E13B_BACCI|GLCA glucan endo-1,3-beta-glucosidase A1 precursor from Bacillus circulans (682 aa), FASTA scores: opt: 122, E(): 1.6, (25.6% identity in 164 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). I6XI06 No conserved hypotheticals I6XI06 I6XI06 NC_000962.3 Mycobrowser_v5 CDS 4150030 4151040 . - 0 Rv3707c Rv3707c Function unknown Conserved hypothetical protein Rv3707c, (MTV025.055c), len: 336 aa. Equivalent to Q9CB79|ML2321 hypothetical protein from Mycobacterium leprae (336 aa), FASTA scores: opt: 1948, E(): 6.7e-110, (81.95% identity in 332 aa overlap); and P41402|YASD_MYCSM hypothetical 35.9 KDA protein in the aspartokinase gene cluster from Mycobacterium smegmatis (333 aa), FASTA scores: opt: 1731, E(): 7.4e-97, (70.85% identity in 333 aa overlap). I6Y4C4 No conserved hypotheticals I6Y4C4 NC_000962.3 Mycobrowser_v5 CDS 4186089 4186577 . + 0 Rv3735 Rv3735 Function unknown Conserved hypothetical protein Rv3735, (MTV025.083), len: 162 aa. Conserved hypothetical protein, highly similar to several bacterial hypothetical proteins e.g. Q9UX41|ORF-C09_016|SSO0651|AAK40956 from Sulfolobus solfataricus (163 aa), FASTA scores: opt: 627, E(): 1.2e-34, (55.9% identity in 161 aa overlap); O26795|MTH699 from Methanobacterium thermoautotrophicum (168 aa), FASTA scores: opt: 616, E(): 6.7e-34, (56.1% identity in 155 aa overlap); |Q9Y9J9|APE2289 from Aeropyrum pernix (191 aa), FASTA scores: opt: 591, E(): 3.4e-32, (54.65% identity in 161 aa overlap) ; etc. Contains PS00435 Peroxidases proximal heme-ligand signature. O69702 No conserved hypotheticals O69702 O69702 O69702 NC_000962.3 Mycobrowser_v5 CDS 4153740 4155674 . + 0 Rv3710 leuA Involved in leucine biosynthesis (at the first step). Catalyzes condensation of acetyl-CoA and 2-oxoisovalerate to form 2-isopropylmalate synthase [catalytic activity: 3-carboxy-3-hydroxy-4-methylpentanoate + CoA = acetyl-CoA + 3-methyl-2-oxobutanoate + H(2)O]. 2-isopropylmalate synthase LeuA (alpha-isopropylmalate synthase) (alpha-IPM synthetase) (IPMS) Rv3710, (MTV025.058), len: 644 aa. LeuA, alpha-isopropylmalate synthase (see citations below), equivalent to Q9CB76|LEUA|ML2324 2-isopropylmalate synthase from Mycobacterium leprae (607 aa), FASTA scores: opt: 3291, E(): 3.7e-192, (80.7% identity in 642 aa overlap). Also highly similar to many e.g. P42455|LEU1_CORGL|LEUA from Corynebacterium glutamicum (Brevibacterium flavum) (616 aa), FASTA scores: opt: 2547, E(): 5.3e-147, (63.25% identity in 645 aa overlap); O31046|LEU1_STRCO|LEUA from Streptomyces coelicolor (573 aa), FASTA scores: opt: 2226, E(): 1.5e-127, (57.8% identity in 616 aa overlap); BAB49833|Q98HN3|MLR2792 from Rhizobium loti (Mesorhizobium loti) (588 aa), FASTA scores: opt: 1849, E(): 1.1e-104, (58.0% identity in 536 aa overlap); etc. Equivalent to AAK48181 from Mycobacterium tuberculosis strain CDC1551 (659 aa) but shorter 15 aa. Contains PS00815 and PS00816 Alpha-isopropylmalate and homocitrate synthases signatures 1 and 2. Belongs to the alpha-IPM synthetase / homocitrate synthase family. K+ is likely the physiological activator; Zn2+ and Cd2+ are inhibitors. P9WQB3 No intermediary metabolism and respiration 1SR9,3FIG,3HPS,3HPX,3HPZ,3HQ1 P96420 P9WQB3 GO:0009098,GO:0003852 2.3.3.13 P9WQB3 NC_000962.3 Mycobrowser_v5 CDS 4162306 4163718 . + 0 Rv3719 Rv3719 Function unknown Conserved protein Rv3719, (MTV025.067), len: 470 aa. Conserved protein, equivalent to O69516|ML2333|MLCB2407.17c hypothetical 51.8 KDA protein from Mycobacterium leprae (459 aa), FASTA scores: opt: 2593, E(): 7.8e-161, (82.75% identity in 458 aa overlap). Also some similarity to Q9CU63|5830417J06RIK hypothetical protein (fragment) from Mus musculus (Mouse) (479 aa) FASTA scores: opt: 454, E(): 6.1e-22, (27.1% identity in 413 aa overlap); Q9HBA8 seladin-1 (unknown) from Homo sapiens (Human) (516 aa), FASTA scores: opt: 444, E(): 2.9e-21, (26.7% identity in 412 aa overlap); O17397|DIMH_CAEEL|F52H2.6 diminuto-like protein from Caenorhabditis elegans (525 aa), FASTA scores: opt: 419, E(): 1.2e-19, (24.4% identity in 434 aa overlap); Q39085|DIM_ARATH|DWF1 cell elongation protein diminuto from Arabidopsis thaliana (Mouse-ear cress) (561 aa) FASTA scores: opt: 318, E(): 4.8e-13, (24.6% identity in 455 aa overlap); etc. Also some similarity to Mycobacterium tuberculosis hypothetical proteins P72056|Rv3790|MTCY13D12.24 (461 aa) FASTA scores: opt: 174, E(): 0.00016; (25.1% identity in 426 aa overlap); and Q50685|Rv2280|MTCY339_30c (459 aa). O69686 No conserved hypotheticals O69686 O69686 GO:0016491,GO:0050660 O69686 NC_000962.3 Mycobrowser_v5 CDS 4190284 4190517 . - 0 Rv3739c PPE67 Function unknown PPE family protein PPE67 Rv3739c, (MTV025.087c), len: 77 aa. PPE67, Member of the Mycobacterium tuberculosis PE family, showing high homology with O53269|Rv3022c|MTV012.36c (82 aa) FASTA scores: opt: 398, E(): 1.2e-19, (74.0% identity in 77 aa overlap); and similar to the N-termini of other PPE proteins e.g. O53265|Rv3018c|MTV012.32c (434 aa) FASTA scores: opt: 398, E(): 4.8e-19, (74.0% identity in 77 aa overlap). ORF ends at the stop codon at position 97470, which is not present in similar ORFs: MTV012_32, or MTCY21B4_4. Sequence homology with MTV012_32, and MTCY21B4_4 continues in the downstream ORF MTV025.086c|Rv3738c|PPE66. Sequence was checked, but no errors were detected. A similar situation, but with a frameshift separating the ORFs, is found in MTV012_36/MTV012_35. Also ORF MTV025.87c shows similarity to MTV03 _14; MTCY6A4_1; MTV035_8; MTV037_17; MLCB2492_30; MTCY261_19; MTCY251_15; MTCY3A2_23; MTCY28_16; etc. Q79FA2 No PE/PPE Q79FA2 Q79FA2 Q79FA2 NC_000962.3 Mycobrowser_v5 CDS 4195886 4196098 . - 0 Rv3745c Rv3745c Function unknown Conserved hypothetical protein Rv3745c, (MTV025.093c), len: 70 aa. Conserved hypothetical protein, highly similar to others e.g. N-terminus of Q9X4E6 hypothetical 13.4 KDA protein from Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides) (124 aa), FASTA scores: opt: 279, E(): 4.4e-14, (59.4% identity in 69 aa overlap); N-terminus of Q9A2A6|CC3660 hypothetical protein from Caulobacter crescentus (172 aa) FASTA scores: opt: 272, E(): 1.9e-13, (63.35% identity in 60 aa overlap); N-terminus of P74345|SLR1628 hypothetical 14.5 KDA protein from Synechocystis sp. strain PCC 6803 (134 aa), FASTA scores: opt: 233, E(): 1.3e-10, (54.85% identity in 62 aa overlap); etc. O69712 No conserved hypotheticals O69712 O69712 O69712 NC_000962.3 Mycobrowser_v5 CDS 4158227 4158922 . + 0 Rv3713 cobQ2 Involved in cobalamin biosynthesis. Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation. Possible cobyric acid synthase CobQ2 Rv3713, (MTV025.061), len: 231 aa. Possible cobQ2, cobyric acid synthase, equivalent to O69521|ML2327|MLCB2407.23c hypothetical 24.5 KDA protein from Mycobacterium leprae (230 aa), FASTA scores: opt: 1313, E(): 4.7e-73, (86.1% identity in 230 aa overlap). Also partially similar to several cobyric acid synthases and hypothetical proteins e.g. Q9FCA0|2SCG58.13 hypothetical 26.2 KDA protein from Streptomyces coelicolor (242 aa), FASTA scores: opt: 639, E(): 6.2e-32, (46.6% identity in 234 aa overlap); Q9ZGG8|COBQ cobyric acid synthase from Heliobacillus mobilis (252 aa), FASTA scores: opt: 501, E(): 1.7e-23, (40.75% identity in 206 aa overlap); BAB58053|SAV1891 hypothetical 27.4 KDA protein from Staphylococcus aureus subsp. aureus Mu50 (243 aa), FASTA scores: opt: 400, E(): 2.3e-17, (35.95% identity in 217 aa overlap); Q9CGJ1|COBQ cobyric acid synthase from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (261 aa), FASTA scores: opt: 353, E(): 1.8e-14, (35.3% identity in 201 aa overlap); O26880|COBQ_METTH|MTH787 probable cobyric acid synthase from Methanobacterium thermoautotrophicum (504 aa), FASTA scores: opt: 201, E(): 5.6e-05, (33.35% identity in 171 aa overlap); etc. Also similar to hypothetical mycobacterial proteins O05811|COBB_MYCTU|Rv2848c|MT2914|MTCY24A1.09 (457 aa) and P71842|Rv0789c|MTCY369.33c (199 aa). Seems to belong to the COBB/COBQ family, COBQ subfamily. I6XI14 No intermediary metabolism and respiration O69680 I6XI14 GO:0009236,GO:0003824 I6XI14 NC_000962.3 Mycobrowser_v5 CDS 4161048 4161773 . + 0 Rv3717 Rv3717 Function unknown Conserved hypothetical protein Rv3717, (MTV025.065), len: 241 aa. Conserved hypothetical protein, equivalent to O69518|MLCB2407.19c (alias Q9CB75|ML2331 256 aa) hypothetical 25.1 KDA protein from Mycobacterium leprae (244 aa), FASTA scores: opt: 1325, E(): 5.7e-74, (81.95% identity in 244 aa overlap). Also similar to Q9KXK7|SCC53.04 putative secreted protein from Streptomyces coelicolor (336 aa), FASTA scores: opt: 536, E(): 1.2e-25, (41.2% identity in 233 aa overlap); and shows similarity with C-terminal end of other proteins e.g. Q9RMZ0|PXO2-42 PXO2-42 protein from Bacillus anthracis (531 aa), FASTA scores: opt: 191, E(): 0.00022, (26.6% identity in 222 aa overlap); Q9RTX0 putative N-acetylmuramoyl-L-alanine amidase (603 aa); Q9LCR4|CWLU CWLU protein from Paenibacillus polymyxa (Bacillus polymyxa) (524 aa), FASTA scores: opt: 141, E(): 0.24, (29.2% identity in 219 aa overlap); etc. Shows similarity with C-terminal end of O53593|CWLM|Rv3915|MTV028.06 putative hydrolase from Mycobacterium tuberculosis (406 aa), FASTA scores: opt: 176, E(): 0.0014, (25.7% identity in 218 aa overlap). I6Y4D2 No conserved hypotheticals O69684 I6Y4D2 GO:0009253,GO:0008745 I6Y4D2 NC_000962.3 Mycobrowser_v5 CDS 4170214 4171143 . + 0 Rv3725 Rv3725 Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv3725, (MTV025.073), len: 309 aa. Possible reductase, similar to various oxidoreductases and hypothetical proteins e.g. O34285|HPNA HPNA protein from Zymomonas mobilis (337 aa), FASTA scores: opt: 317, E(): 6.1e-11, (30.5% identity in 272 aa overlap); Q9SZB3|F17M5.120|AT4G33360|AAK49584 hypothetical 37.9 KDA protein from Arabidopsis thaliana (Mouse-ear cress) (344 aa), FASTA scores: opt: 314, E(): 9.1e-11, (30.35% identity in 267 aa overlap); AAK59445|AT4G33360 putative dihydrokaempferol 4-reductase from Arabidopsis thaliana (Mouse-ear cress) (332 aa), FASTA scores: opt: 313, E(): 1e-10, (30.8% identity in 263 aa overlap); Q9FSC6|CCR cinnamoyl-CoA reductase from Populus trichocarpa (Western balsam poplar) (338 aa), FASTA scores: opt: 305, E(): 2.9e-10, (30.3% identity in 274 aa overlap); Q9M631 cinnamoyl CoA reductase from Populus tremuloides (Quaking aspen) (337 aa), FASTA scores: opt: 291, E(): 1.8e-09, (30.15% identity in 272 aa overlap); P73212|DFRA_SYNY3|LR1706 putative dihydroflavonol-4-reductase (dihydrokaempferol 4-reductase) from Synechocystis sp. strain PCC 6803 (343 aa), FASTA scores: opt: 278, E(): 1e-08, (29.35% identity in 259 aa overlap); etc. Also some similarity to proteins from Mycobacterium tuberculosis e.g. P96816|Rv0139|MTCI5.13 hypothetical protein (340 aa) FASTA scores: opt: 234, E(): 3.2e-06, (28.25% identity in 269 aa overlap); and O06373|galE1|Rv3634c|MTCY15C10.18 probable UDP-glucose 4-epimerase (314 aa) (27.3% identity in 194 aa overlap). O69692 No intermediary metabolism and respiration O69692 O69692 GO:0050662,GO:0055114,GO:0016491,GO:0044237 1.-.-.- O69692 NC_000962.3 Mycobrowser_v5 CDS 4159889 4160500 . - 0 Rv3715c recR May play a role in DNA repair. It seems to be involved in an RECBC-independent recombinational process of DNA repair. It may act with RECF|Rv0003 (and RECO|Rv2362c ?) for modulating assembly of disassembly of RECA filaments. Probable recombination protein RecR Rv3715c, (MTV025.063c), len: 203 aa. Probable recR, recombination protein (see citation below), equivalent to O69520|RECR_MYCLE|ML2329|MLCB2407.21 recombination protein from Mycobacterium leprae (203 aa), FASTA scores: opt: 1246, E(): 9.2e-71, (91.6% identity in 202 aa overlap). Also highly similar to many e.g. Q9XAI4|RECR_STRCO|SC66T3.29c from Streptomyces coelicolor (199 aa), FASTA scores: opt: 952, E(): 1.9e-52, (68.3% identity in 202 aa overlap); P24277|RECR_BACSU|RECM|recd from Bacillus subtilis (198 aa), FASTA scores: opt: 696, E(): 1.8e-36, (50.5% identity in 198 aa overlap); Q9ZNA2|RECR_DEIRA|DR0198 from Deinococcus radiodurans (220 aa), FASTA scores: opt: 673, E(): 5.2e-35, (49.75% identity in 195 aa overlap); etc. Belongs to the RECR family. P9WHI3 No information pathways P65990 P9WHI3 GO:0006310,GO:0006281,GO:0046872,GO:0003677 P9WHI3 NC_000962.3 Mycobrowser_v5 CDS 4160512 4160913 . - 0 Rv3716c Rv3716c Function unknown Conserved protein Rv3716c, (MTV025.064c), len: 133 aa. Conserved protein, equivalent to O69519|Y1B6_MYCLE|ML2330|MLCB2407.20 hypothetical 11.9 KDA protein from Mycobacterium leprae (116 aa), FASTA scores: opt: 616, E(): 2.6e-21, (84.55% identity in 110 aa overlap). Also highly similar to hypothetical ~12 kDa proteins in the vicinity of recR from other bacteria e.g. Q9XAI3|YT3D_STRCO|SC66T3.30c hypothetical 11.7 KDA protein from Streptomyces coelicolor (115 aa), FASTA scores: opt: 379, E(): 9.5e-11, (50.8% identity in 122 aa overlap); BAB56641|SAV0479 conserved hypothetical protein from Staphylococcus aureus subsp. aureus Mu50 (105 aa) FASTA scores: opt: 295, E(): 4.9e-07, (41.75% identity in 103 aa overlap); Q99WC4P24281|YAAK_BACSU hypothetical 11.8 KDA protein in DNAZ-RECR intergenic region from Bacillus subtilis (107 aa), FASTA scores: opt: 272, E(): 5.3e-06, (39.4% identity in 104 aa overlap); P17577|YBAB_ECOLI|B0471|Z0588|ECS0524 from Escherichia coli strain K and O157:H7 (109 aa), FASTA scores: opt: 256, E(): 2.8e-05, (38.0% identity in 100 aa overlap); etc. Contains probable coiled-coil domain from aa 1-40. Seems to belong to the UPF0133 family. P9WNR9 No conserved hypotheticals P0A654 P9WNR9 P9WNR9 NC_000962.3 Mycobrowser_v5 CDS 4161815 4162258 . - 0 Rv3718c Rv3718c Function unknown Conserved protein Rv3718c, (MTV025.066c), len: 147 aa. Conserved protein, equivalent to O69517|ML2332|MLCB2407.18 hypothetical 15.5 KDA protein from Mycobacterium leprae (145 aa), FASTA scores: opt: 780, E(): 1.4e-44, (81.95% identity in 144 aa overlap). Also highly similar to Q9ZBJ2|SC9C7.18 conserved hypothetical protein from Streptomyces coelicolor (147 aa) FASTA scores: opt: 475, E(): 1.7e-24, (52.05% identity in 146 aa overlap); and showing some similarity to various proteins e.g. P27538|PR2_PETCR pathogenesis-related protein 2 from Petroselinum crispum (Parsley) (Petroselinum hortense) (158 aa); P92918|ALL2_APIGR major allergen API G 2 from Apium graveolens (Celery) (159 aa); etc. Thought to be differentially expressed within host cells (see citation below). I6XI16 No conserved hypotheticals O69685 I6XI16 I6XI16 NC_000962.3 Mycobrowser_v5 CDS 4216404 4216730 . - 0 Rv3771c Rv3771c Function unknown Conserved hypothetical protein Rv3771c, (MTCY13D12.05c), len: 108 aa. Hypothetical protein, highly similar, but shorter 81 aa, to P71640|Rv2811|MTCY16B7.32c hypothetical 21.1 KDA protein from Mycobacterium tuberculosis (202 aa), FASTA scores: opt: 469, E(): 2.7e-25, (73.15% identity in 108 aa overlap) P72038 No conserved hypotheticals P72038 NC_000962.3 Mycobrowser_v5 ncRNA 4168154 4168281 . - 0 MTB000059 C8 Unknown Possible 4.5S RNA in signal recognition particle (small cytoplasmic RNA) (SC-RNA) C8, possible 4.5S RNA (See Arnvig and Young, 2009; DiChiara et al., 2010), part of signal recognition particle with protein Ffh. Alternate 3'-ends at positions 4168212 and 4168224. No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 4322326 4323234 . + 0 Rv3848 Rv3848 Unknown Probable conserved transmembrane protein Rv3848, (MTCY01A6.20c), len: 302 aa. Probable conserved transmembrane protein, similar to hypothetical (transmembrane) proteins e.g. Q9HVG2|PA4629 hypothetical protein from Pseudomonas aeruginosa (192 aa), FASTA scores: opt: 304, E(): 5.3e-11, (35.05% identity in 174 aa overlap); Q9A5S7|CC2370 hypothetical protein from Caulobacter crescentus (207 aa), FASTA scores: opt: 285, E(): 7.4e-10, (29.9% identity in 184 aa overlap); Q9KY43|SCC8A.05c putative integral membrane protein from Streptomyces coelicolor (193 aa), FASTA scores: opt: 245, E(): 1.6e-07, (32.8% identity in 195 aa overlap); etc. P96229 No cell wall and cell processes P96229 P96229 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 4166821 4168128 . - 0 Rv3722c Rv3722c Function unknown Conserved protein Rv3722c, (MTV025.070c), len: 435 aa. Conserved protein, equivalent to O69513|MLCB2407.14 (alias Q9CB73|ML2336, 463 aa) hypothetical 46.8 KDA protein from Mycobacterium leprae (426 aa), FASTA scores: opt: 2505, E(): 8.3e-154, (87.25% identity in 424 aa overlap). Also highly similar to Q9RU17|DR1579 conserved hypothetical protein from Deinococcus radiodurans (452 aa), FASTA scores: opt: 1162, E(): 3.1e-67, (44.8% identity in 422 aa overlap); and partially similar to Q9I371|PA1654 probable aminotransferase from Pseudomonas aeruginosa (388 aa) FASTA scores: opt: 162, E(): 0.0078, (25.85% identity in 348 aa overlap) and other aminotransferases. N-terminus extended since first submission (previously 408 aa). O69689 No conserved hypotheticals O69689 GO:0030170,GO:0003824 O69689 NC_000962.3 Mycobrowser_v5 CDS 4171421 4172614 . + 0 Rv3726 Rv3726 Function unknown; probably involved in cellular metabolism. Possible dehydrogenase Rv3726, (MTV025.074), len: 397 aa. Possible dehydrogenase, similar to many e.g. O34788|YDJL dehydrogenase from Bacillus subtilis (346 aa) FASTA scores: opt: 401, E(): 3.4e-17, (29.6% identity in 395 aa overlap); Q59696|ADH 2,3-butanediol dehydrogenase from seudomonas putida (362 aa), FASTA scores: opt: 326, E(): 1.3e-12, (29.45% identity in 387 aa overlap); AAG59541|YJJN putative oxidoreductase from Escherichia coli strain EDL933 (345 aa), FASTA scores: opt: 325, E(): 1.5e-12, (30.85% identity in 256 aa overlap); Q9HWM8|PA4153 2,3-butanediol dehydrogenase from Pseudomonas aeruginosa (363 aa), FASTA scores: opt: 324, E(): 1.8e-12, (30.5% identity in 387 aa overlap); etc. O69693 No intermediary metabolism and respiration O69693 O69693 GO:0016491,GO:0008270,GO:0055114 1.-.-.- O69693 NC_000962.3 Mycobrowser_v5 CDS 4212293 4212982 . + 0 Rv3766 Rv3766 Unknown Hypothetical protein Rv3766, (MTV025.114), len: 229 aa. Hypothetical unknown protein. Segment 183 to 229 highly similar to C-terminal part of O06288|Rv3594|MTCY07H7B.28c conserved hypothetical protein from Mycobacterium tuberculosis (275 aa), FASTA scores: opt: 128, E(): 0.92, (46.8% identity in 47 aa overlap). O69731 No conserved hypotheticals O69731 O69731 NC_000962.3 Mycobrowser_v5 CDS 4386365 4386814 . - 0 Rv3901c Rv3901c Unknown Possible membrane protein Rv3901c, (MTCY15F10.11), len: 149 aa. Possible membrane protein (hydrophobic stretch from ~30-52), showing some similarity with O53200|Rv2473|MTV008.29 hypothetical 25.1 KDA protein from Mycobacterium tuberculosis (238 aa), FASTA scores: opt: 147, E(): 0.036, (31.35% identity in 134 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O05444 No cell wall and cell processes O05444 NC_000962.3 Mycobrowser_v5 CDS 4174873 4178070 . + 0 Rv3728 Rv3728 Unknown, but seems involved in efflux system (probably sugar or drug transport). Probable conserved two-domain membrane protein Rv3728, (MTV025.076), len: 1065 aa. Probable conserved transmembrane protein organised into two domains. Domain comprising the first ~510 aa residues is similar to various multidrug resistance and efflux proteins and contains sugar transport protein signature 1 (PS00216). Domain corresponding to the last 550 aa residues contains cyclic nucleotide-binding domain signature 2 (PS00889) and is very similar to Q50733|YP65_MYCTU|Rv2565|MT2641|MTCY9C4.03c hypothetical 62.1 kDa protein from Mycobacterium tuberculosis (31.0% identity in 546 aa overlap). Highly similar to O05884|Rv3239c|MTCY20B11.14c probable transmembrane transport protein from Mycobacterium tuberculosis (1048 aa) FASTA scores: opt: 4328, E(): 5e-201, (64.15% identity in 1046 aa overlap). N-terminal end similar to P71879|Rv2333c|MTCY3G12.01|MTCY98.02c (537 aa); P71836|Rv0783c|MTCY369.27c (540 aa); and O07753|Rv1877|MTCY180.41c (687 aa). Seems belong to the sugar transporter family. Possibly member of major facilitator superfamily (MFS). O69695 No cell wall and cell processes O69695 O69695 GO:0006629,GO:0046677,GO:0015904,GO:0015520,GO:0055085,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 4178285 4180615 . + 0 Rv3729 Rv3729 Function unknown; probably involved in cellular metabolism. Possible transferase Rv3729, (MTV025.077), len: 776 aa. Conserved hypothetical protein, possible transferase, similar to several hypothetical proteins and various transferases e.g. O26919|MTH831 molybdenum cofactor biosynthesis MOAA homolog from Methanobacterium thermoautotrophicum (497 aa), FASTA scores: opt: 697, E(): 4.8e-34, (30.7% identity in 492 aa overlap); Q58036|Y619_METJA|MJ0619 hypothetical protein from Methanococcus jannaschii (506 aa), FASTA scores: opt: 670, E(): 2e-32, (30.6% identity in 497 aa overlap); O27968|AF2316 conserved hypothetical protein from Archaeoglobus fulgidus (518 aa), FASTA scores: opt: 477, E(): 6.4e-21, (29.4% identity in 500 aa overlap); BAB60102|TVG0985801 molybdenum cofactor biosynthesis protein from Thermoplasma volcanium (606 aa), FASTA scores: opt: 402, E(): 2.1e-16, (28.1% identity in 509 aa overlap); etc. C-terminus similar to methyltransferases e.g. Q9S0N6|AVED C5-O-methyltransferase from Streptomyces avermitilis (283 aa), FASTA scores: opt: 298, E(): 1.9e-10, (31.5% identity in 292 aa overlap). Also similar to the Mycobacterium tuberculosis proteins P71673|YE05_MYCTU|Rv1405c|MT1449|MTCY21B4.22c (274 aa); and Q50584|Rv1523|MTCY19G5.05c. O69696 No intermediary metabolism and respiration O69696 O69696 GO:0008152,GO:0008168,GO:0051536 2.-.-.- O69696 NC_000962.3 Mycobrowser_v5 CDS 4180680 4181720 . - 0 Rv3730c Rv3730c Function unknown Conserved hypothetical protein Rv3730c, (MTV025.078c), len: 346 aa. Conserved hypothetical protein, highly similar to Q9XAM1|SC4C6.19 hypothetical 38.5 KDA protein from Streptomyces coelicolor (341 aa), FASTA scores: opt: 1313, E(): 2.2e-75, (59.25% identity in 336 aa overlap); and similar to C-terminal end of putative ATP-dependent DNA ligases e.g. BAB49297|MLL2077 from Rhizobium loti (Mesorhizobium loti) (833 aa), FASTA scores: opt: 550, E(): 5.3e-27, (31.3% identity in 294 aa overlap); and BAB54816|MLL9625 from Rhizobium loti (Mesorhizobium loti) plasmid pMLb (883 aa) FASTA scores: opt: 492, E(): 2.5e-23, (33.7% identity in 291 aa overlap); etc. Also similar to the hypothetical proteins e.g. Q9ZC15|SC1E6.07 hypothetical 34.9 KDA protein from Streptomyces coelicolor (319 aa) FASTA scores: opt: 537, E(): 1.5e-26, (34.95% identity in 292 aa overlap); Q9XAF7|SC6G9.25 hypothetical 32.1 KDA protein from Streptomyces coelicolor (293 aa), FASTA scores: opt: 474, E(): 1.3e-22, (33.75% identity in 302 aa overlap); etc. Also highly similar to P95226|Rv0269c|MTCY06A4.13c hypothetical 44.0 KDA protein from Mycobacterium tuberculosis (397 aa), FASTA scores: opt: 940, E(): 7.7e-52, (50.3% identity in 312 aa overlap). O69697 No conserved hypotheticals O69697 O69697 GO:0006269,GO:0003896 O69697 NC_000962.3 Mycobrowser_v5 CDS 4205538 4205840 . + 0 Rv3760 Rv3760 Unknown Possible conserved membrane protein Rv3760, (MTV025.108), len: 100 aa. Possible conserved membrane protein, equivalent to Q50094|ML2366|MLCB12.11c putative membrane protein from Mycobacterium leprae (113 aa), FASTA scores: opt: 423, E(): 1.2e-20, (67.7% identity in 99 aa overlap). Also similar with Q9JST1|NMA2149 putative inner membrane hypothetical protein from Neisseria meningitidis (serogroup A) (104 aa), FASTA scores: opt: 113, E(): 0.95, (33.85% identity in 62 aa overlap); and showing similarity with Q9ZAX7 ABC transporter membrane protein subunit from Streptococcus mutans (498 aa), FASTA scores: opt: 108, E(): 6.7, (42.35% identity in 85 aa overlap) (similarity at C-terminus); and P33108|SECY_MICLU preprotein translocase SECY subunit from Micrococcus luteus (Micrococcus lysodeikticus) (436 aa), FASTA scores: opt: 106, E(): 8.2, (29.05% identity in 86 aa overlap). Equivalent to AAK48231 from Mycobacterium tuberculosis strain CDC1551 (117 aa) but shorter 17 aa. O69726 No cell wall and cell processes O69726 O69726 O69726 NC_000962.3 Mycobrowser_v5 CDS 4182934 4183992 . + 0 Rv3732 Rv3732 Function unknown Conserved protein Rv3732, (MTV025.080), len: 352 aa. Conserved protein. The region between aa 175-352 is highly similar to the region between aa 72-257 of Q9KH39 hypothetical 55.5 KDA protein from Mycobacterium smegmatis (511 aa), FASTA scores: opt: 1122, E(): 7.3e-63, (98.85% identity in 176 aa overlap). Also shows some similarity with Q55304 hypotheticalk protein from Synechocystis sp. strain PCC 6803 (387 aa), FASTA scores: opt: 201, E(): 2.7e-05, (27.1% identity in 251 aa overlap); and P74254|SLR1173 hypothetical 52.5 KDA protein from Synechocystis sp. strain PCC 6803 (463 aa), FASTA scores: opt: 201, E(): 3.1e-05, (27.1% identity in 251 aa overlap). Also slightly similar to MTCY01B2_21 and DPO1_MYCTU DNA polymerase I. O69699 No conserved hypotheticals O69699 O69699 NC_000962.3 Mycobrowser_v5 CDS 4198205 4198597 . - 0 Rv3750c Rv3750c Sequence excision. Possible excisionase Rv3750c, (MTV025.098c), len: 130 aa. Possible excisionase, similar to others e.g. Q9LCU5 putative excisionase from Arthrobacter sp. TM1 (174 aa) FASTA scores: opt: 297, E(): 1.2e-12, (40.35% identity in 114 aa overlap); O85865 putative excisionase from Sphingomonas aromaticivorans plasmid pNL1 (152 aa), FASTA scores: opt: 223, E(): 7.3e-08, (39.15% identity in 97 aa overlap); Q9XBH1|xis excisionase from Bacteroides fragilis (124 aa) FASTA scores: opt: 128, E(): 0.1, (30.7% identity in 88 aa overlap); etc. Also some similarity to transcriptional regulators. Also similar to Mycobacterium tuberculosis hypothetical proteins e.g. P71902|YN10_MYCTU|Rv2310|MT2372|MTCY3G12.24c (114 aa) FASTA scores: opt: 224, E(): 4.9e-08, (42.7% identity in 82 aa overlap). Contains helix-turn-helix motif at aa 55-76 (Score 1925,+5.74 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O69717 No insertion seqs and phages O69717 GO:0000166,GO:0003677 NC_000962.3 Mycobrowser_v5 CDS 4198874 4199089 . + 0 Rv3751 Rv3751 Sequence integration. Probable integrase (fragment) Rv3751, (MTV025.099), len: 71 aa. Probable integrase (fragment), similar to part of many e.g. Q48908 integrase (fragment) from Mycobacterium paratuberculosis (191 aa), FASTA scores: opt: 206, E(): 5.5e-08, (57.65% identity in 59 aa overlap); Q9ZWV7|int integrase from Corynephage 304L (395 aa), FASTA scores: opt: 156, E(): 0.00036, (45.75% identity in 59 aa overlap); Q9K722|BH3551 integrase (phage-related protein) from Bacillus halodurans (378 aa), FASTA scores: opt: 151, E(): 0.00079, (46.15% identity in 52 aa overlap); etc. Also similarity with various conjugative transposons. Also similar to Mycobacterium tuberculosis hypothetical proteins e.g. P71903|Rv2309c|MTCY3G12.25 (151 aa), FASTA scores: opt: 193, E(): 3.8e-07, (50.85% identity in 59 aa overlap); O53403|Rv1055|MTV017.08 (78 aa), FASTA scores: opt: 171, E(): 7.8e-06, (54.15% identity in 48 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O69718 No insertion seqs and phages O69718 O69718 GO:0015074,GO:0006310,GO:0003677 NC_000962.3 Mycobrowser_v5 CDS 4187699 4189288 . + 0 Rv3737 Rv3737 Unknown Probable conserved transmembrane protein Rv3737, (MTV025.085), len: 529 aa. Probable conserved transmembrane protein, similar to others and also some hypothetical proteins e.g. AAK61331|THRE threonine export carrier from Corynebacterium glutamicum (Brevibacterium flavum) (489 aa), FASTA scores: opt: 773, E(): 1.8e-36, (37.25% identity in 424 aa overlap); Q9X8J0|SCE9.17 putative membrane protein from Streptomyces coelicolor (578 aa), FASTA scores: opt: 642, E(): 5.4e-29, (31.6% identity in 481 aa overlap) (shorter 119 aa at N-terminus); Q9CJU6|PM1895 hypothetical protein from Pasteurella multocida (262 aa), FASTA scores: opt: 233, E(): 4.1e-06, (25.0% identity in 256 aa overlap); Q9S267|SCI30A.06 putative integral membrane protein from Streptomyces coelicolor (297 aa), FASTA scores: opt: 163, E(): 0.042, (29.65% identity in 263 aa overlap); etc. Also partially similar to O05435|Rv3910|MTCY15F10.01c|MTV028.01 hypothetical 123.6 KDA protein from Mycobacterium tuberculosis (1184 aa) (34.4% identity in 125 aa overlap). O69704 No cell wall and cell processes O69704 O69704 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 4189285 4190232 . - 0 Rv3738c PPE66 Function unknown PPE family protein PPE66 Rv3738c, (MTV025.086c), len: 315 aa. PPE66, Member of the Mycobacterium tuberculosis PPE family, highly similar to many e.g. O53265|Rv3018c|MTV012.32c (434 aa), FASTA scores: opt: 464, E(): 2.2e-17, (47.05% identity in 338 aa overlap). Probably a continuation of the upstream ORF MTV025.87c|Rv3739c|PPE67. At position 97470-72 a stop codon is present which interrupts a possibly longer ORF, observed in related ORFs MTV012_32 or MTCY21B4_4. The sequence has been checked and no errors were detected. A similar situation, but with a frameshift separating the ORFs is found in MTV012_36/MTV012_35. Sequence similarity is also seen with MTCY251_15; MTCY261_19; MLCB2492_30 from Mycobacterium leprae; MTCY10G2_10; MTY21C12_9; MTCI125_26; MTCY164_36; MTCY6A4_1. P9WHX1 No PE/PPE Q79FA3 P9WHX1 GO:0005886,GO:0016021 P9WHX1 NC_000962.3 Mycobrowser_v5 tRNA 4199131 4199217 . - 0 MTB000047 serX - tRNA-Ser (CGA) serX, tRNA-Ser, anticodon cga, length = 87. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 4193391 4195373 . - 0 Rv3743c ctpJ Cation-transporting ATPase; possibly catalyzes the transport of a undetermined cation (possibly cadmium) with hydrolyse of ATP [catalytic activity: ATP + H(2)O + undetermined cation(in) = ADP + phosphate + undetermined cation(out)]. Probable cation transporter P-type ATPase CtpJ Rv3743c, (MTV025.091c), len: 660 aa. Probable ctpJ, cation-transporting P-type ATPase, transmembrane protein highly similar to others e.g. Q9ZBF3|SC9B5.27 putative cation-transporting ATPase from Streptomyces coelicolor (638 aa), FASTA scores: opt: 1635, E(): 2.5e-86, (62.25% identity in 63.95 aa overlap); Q59997|CADA|SLR0797 cadmium-transporting ATPase from Synechocystis sp. strain PCC 6803 (642 aa), FASTA scores: opt: 1474, E(): 4.3e-77, (42.4% identity in 604 aa overlap); P30336|CADA_BACFI probable cadmium-transporting ATPase from Bacillus firmus (723 aa), FASTA scores: opt: 1327, E(): 1.3e-68, (36.6% identity in 626 aa overlap); etc. Also highly similar to O53160|CTPD_MYCTU|Rv1469|MT1515|MTV007.16 probable cation-transporting P-type ATPase D from Mycobacterium tuberculosis (657 aa), FASTA scores: opt: 1845, E(): 2.3e-98, (55.85% identity in 650 aa overlap). Contains PS00154 E1-E2 ATPases phosphorylation site and PS01229 Hypothetical family signature 2. Belongs to the cation transport ATPases family (E1-E2 ATPases). Transcription is repressed by NmtR (See Cavet et al., 2002). P9WPT7 No cell wall and cell processes O69710 P9WPT7 GO:0006754,GO:0015662,GO:0005261,GO:0016021,GO:0046872,GO:0030001,GO:0005524 3.6.1.- P9WPT7 NC_000962.3 Mycobrowser_v5 CDS 4190833 4192179 . - 0 Rv3740c Rv3740c May be involved in synthesis of triacylglycerol Possible triacylglycerol synthase (diacylglycerol acyltransferase) Rv3740c, (MTV025.088c), len: 448 aa. Possible triacylglycerol synthase (See Daniel et al., 2004), highly similar to several other Mycobacterium tuberculosis hypothetical proteins e.g. O69701|Y1D4_MYCTU|Rv3734c|MT3839|MTV025.082c (454 aa) FASTA scores: opt: 1917, E(): 2.3e-112, (61.4% identity in 451 aa overlap); Q50680|YM85_MYCTU|Rv2285|MT2343|MTCY339.25c (445 aa) FASTA scores: opt: 858, E(): 3.4e-46, (37.4% identity in 460 aa overlap); Q10554|Y895_MYCTU|Rv0895|MT0919|MTCY31.23 (505 aa), FASTA scores: opt: 767, E(): 1.9e-40, (44.3% identity in 467 aa overlap); MTCY31_25; MTCY28_26; MTCY493_29; MTCY21B4_43; MTCY8D5_16; MTCY3A2_28; MTV013_8; MTY13E12_33; MTV013_9; MTY20B11_9; etc. Also similar to Q9RIU8|SCM11.13c hypothetical 47.1 KDA protein from Streptomyces coelicolor (446 aa), FASTA scores: opt: 319, E(): 1.7e-12, (30.9% identity in 453 aa overlap). P9WKA5 No lipid metabolism O69707 P9WKA5 GO:0006071,GO:0008610,GO:0004144 2.3.1.20 P9WKA5 NC_000962.3 Mycobrowser_v5 CDS 4278394 4279215 . - 0 Rv3813c Rv3813c Function unknown Conserved protein Rv3813c, (MTCY409.17), len: 273 aa. Conserved protein, equivalent to Q9CDB9|ML0089 hypothetical protein from Mycobacterium leprae (281 aa) FASTA scores: opt: 1479, E(): 9.6e-81, (80.45% identity in 271 aa overlap); and similar to Q98LI0|MLL1014 from (280 aa). Also similar to many hypothetical proteins from several organisms e.g. Q9ZBX2|SCD78.27c from Streptomyces coelicolor (280 aa), FASTA scores: opt: 597, E(): 2.2e-28, (43.25% identity in 266 aa overlap); Q9RXR7|DR0240 from Deinococcus radiodurans (284 aa), FASTA scores: opt: 543, E(): 3.5e-25, (38.65% identity in 264 aa overlap); Q99YH5|SPY1700 from Streptococcus pyogenes (274 aa) FASTA scores: opt: 373, E(): 4.3e-15, (30.75% identity in 270 aa overlap); P70947|YITU from Bacillus subtilis (270 aa) FASTA scores: opt: 353, E(): 6.5e-14, (30.0% identity in 280 aa overlap); etc. O07810 No conserved hypotheticals O07810 O07810 GO:0008152,GO:0016787 O07810 NC_000962.3 Mycobrowser_v5 CDS 4192179 4192853 . - 0 Rv3741c Rv3741c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv3741c, (MTV025.089c), len: 224 aa. Possible oxidoreductase, probably combines with product of upstream ORF MTV025.090c to form a functional monooxygenase, highly similar to C-terminal end of various oxidoreductases e.g. Q9APW3 aromatic-ring hyroxylase from Pseudomonas aeruginosa (508 aa), FASTA scores: opt: 549, E(): 5.9e-28, (56.1% identity in 155 aa overlap); Q9A588|CC2569 monooxygenase (flavin-binding family) from Caulobacter crescentus (498 aa), FASTA scores: opt: 487, E(): 5.6e-24, (39.55% identity in 225 aa overlap); Q9RZT0|DRB0033 arylesterase/monoxygenase from Deinococcus radiodurans (833 aa), FASTA scores: opt: 460, E(): 4.7e-22, (38.5% identity in 226 aa overlap); etc. Also similar to C-terminal end of Mycobacterium tuberculosis proteins (generally monooxygenases) e.g. P96223|Rv3854c|MTCY01A6.14 hypothetical 55.3 KDA protein (489 aa), FASTA scores: opt: 542, E(): 1.6e-27, (50.0% identity in 162 aa overlap); O53762|Rv0565c|MTV039.03c putative monoxygenase (486 aa), FASTA scores: opt: 462, E(): 2.2e-22, (37.15% identity in 226 aa overlap); O53300|Rv3083|MTV013.04 monoxygenase (495 aa), FASTA scores: opt: 462, E(): 2.2e-22, (45.65% identity in 173 aa overlap); etc. Note similarity to MTCY01A6.14 and MTV013.04 continue in upstream ORF (MTV025.090c) after a gap of ~100 aa. O69708 No intermediary metabolism and respiration O69708 GO:0016491,GO:0055114 1.-.-.- O69708 NC_000962.3 Mycobrowser_v5 CDS 4215881 4216063 . - 0 Rv3770A Rv3770A Possibly required for the transposition of an insertion element. Probable remnant of a transposase Rv3770A, len: 60 aa. Probable remnant of a transposase, similar to many e.g. Rv2812|MTCY16B7.31c|Z81331_17 IS1604 putative transposase from Mycobacterium tuberculosis (469 aa), FASTA scores: opt: 204, E(): 1e-07, (80.5% identity in 41 aa overlap). Continuation of Rv3770B. L7N6A0 No insertion seqs and phages L7N6A0 NC_000962.3 Mycobrowser_v5 CDS 4201289 4201888 . - 0 Rv3755c Rv3755c Function unknown Conserved protein Rv3755c, (MTV025.103c), len: 199 aa. Conserved protein showing similarity to CAC47343|SMC03980 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (196 aa) FASTA scores: opt: 244, E(): 4.1e-09, (30.9% identity in 191 aa overlap); Q9I2B5|PA1994 from Pseudomonas aeruginosa (187 aa), FASTA scores: opt: 226, E(): 6e-08, (29.9% identity in 194 aa overlap); and Q98N73|MLR0268 hypothetical protein (183 aa), FASTA scores: opt: 234, E(): 1.8e-08, (27.05% identity in 185 aa overlap). O86358 No conserved hypotheticals O86358 O86358 O86358 NC_000962.3 Mycobrowser_v5 CDS 4202610 4203299 . - 0 Rv3757c proW Thought to be involved in active transport of osmoprotectant (glycine betaine/carnitine/choline/L-proline) across the membrane (import). Responsible for the translocation of the substrate across the membrane. Possible osmoprotectant (glycine betaine/carnitine/choline/L-proline) transport integral membrane protein ABC transporter ProW Rv3757c, (MTV025.105c), len: 229 aa. Possible proW, osmoprotectant transport integral membrane protein ABC transporter (see citation below), similar to osmoprotection proteins (proW, proZ) involved in glycine betaine/L-proline/choline transport, e.g. BAB58607|Q99RI6|OPUCD|SA2234|SAV2445 OPUCD protein (probable glycine betaine/carnitine/choline ABC transporter) from Staphylococcus aureus (231 aa) FASTA scores: opt: 364, E(): 7.1e-15, (30.0% identity in 220 aa overlap); Q45461|OPBB_BACSU|OPUBB|prow choline transport system permease protein (mediate the uptake of choline for synthesis of the osmoprotectant glycine betaine) from Bacillus subtilis (217 aa), FASTA scores: opt: 348, E(): 6.2e-14, (31.05% identity in 206 aa overlap); O34878|OPCB_BACSU|OPUCB glycine betaine/carnitine/choline transport system permease protein from Bacillus subtilis (217 aa), FASTA scores: opt: 343, E(): 1.2e-13, (30.1% identity in 206 aa overlap); O34742|OPCD_BACSU|OPUCD glycine betaine/carnitine/choline transport system permease protein from Bacillus subtilis (229 aa) FASTA scores: opt: 337, E(): 2.9e-13, (31.1% identity in 193 aa overlap); etc. Could belong to the CYSTW subfamily. O69723 No virulence, detoxification, adaptation O69723 O69723 GO:0005886,GO:0006810,GO:0005215,GO:0016021 O69723 NC_000962.3 Mycobrowser_v5 CDS 4273739 4274593 . + 0 Rv3810 pirG Surface-exposed protein required for multiplication and intracellular growth. Seems to play a role in virulence. Exported repetitive protein precursor PirG (cell surface protein) (EXP53) Rv3810, (MTV026.15), len: 284 aa. PirG (alternate gene names: P36 or erp for Exported Repeated Protein), cell surface protein precursor (see citations below), equivalent to P19361|28KD_MYCLE|ML0091 28 KDA antigen precursor from Mycobacterium leprae (236 aa), FASTA scores: opt: 555, E(): 9.8e-18, (52.65% identity in 281 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WIQ7 No cell wall and cell processes P0A5P4 P9WIQ7 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 4325074 4325478 . + 0 Rv3852 hns Function unknown Possible histone-like protein Hns Rv3852, (MTCY01A6.16c), len: 134 aa. Possible hns, histone-like protein, equivalent to Q9CDD1|HNS|ML0067 histone-like protein from Mycobacterium leprae (121 aa), FASTA scores: opt: 341, E(): 4.3e-09, (51.5% identity in 134 aa overlap). Shows some similarity with other histone-like proteins e.g. O65795|HIS1 histone H1 from Triticum aestivum (Wheat) (288 aa), FASTA scores: opt: 183, E(): 0.091, (34.85% identity in 109 aa overlap); etc. I6YHB0 No information pathways I6YHB0 NC_000962.3 Mycobrowser_v5 CDS 4282449 4283999 . + 0 Rv3818 Rv3818 Unknown Unknown protein Rv3818, (MTCY409.12c), len: 516 aa. Unknown protein. P9WH21 No conserved hypotheticals O07805 P9WH21 GO:0046872,GO:0055114,GO:0016491,GO:0051537 P9WH21 NC_000962.3 Mycobrowser_v5 CDS 4204426 4205373 . - 0 Rv3759c proX Thought to be involved in active transport of osmoprotectant (glycine betaine/carnitine/choline/L-proline) across the membrane (import). Possible osmoprotectant (glycine betaine/carnitine/choline/L-proline) binding lipoprotein ProX Rv3759c, (MTV025.107c), len: 315 aa. Possible proX, osmoprotectant-binding lipoprotein component of osmoprotectant transport system (see citation below), similar to osmoprotection proteins (proX) involved in glycine betaine/L-proline/choline transport, e.g. AAK79442|CAC1474 proline/glycine betaine ABC transport system periplasmic component from Clostridium acetobutylicum (303 aa), FASTA scores: opt: 308, E(): 1.2e-11, (27.4% identity in 314 aa overlap); Q9X4J2|PROXL|SCE19A.33 PROXL protein from Streptomyces coelicolor (322 aa), FASTA scores: opt: 302, E(): 3e-11, (27.2% identity in 327 aa overlap); O29280|AF0982 osmoprotection protein (PROX) from Archaeoglobus fulgidus (292 aa), FASTA scores: opt: 235, E(): 3.4e-07, (23.15% identity in 285 aa overlap); etc. Also similar to MTV006_16 hypothetical protein from Mycobacterium tuberculosis, and MLU15180_43 hypothetical protein from Mycobacterium leprae. Equivalent to AAK48230 from Mycobacterium tuberculosis strain CDC1551 (343 aa) but shorter 28 aa. Contains probable N-terminal signal sequence. O69725 No virulence, detoxification, adaptation O69725 O69725 GO:0006810,GO:0005215 O69725 NC_000962.3 Mycobrowser_v5 CDS 4205862 4206917 . - 0 Rv3761c fadE36 Function unknown, but possibly involvement in lipid degradation. Possible acyl-CoA dehydrogenase FadE36 Rv3761c, (MTV025.109c), len: 351 aa. Possible fadE36, acyl-CoA dehydrogenase, similar to many conserved hypothetical proteins and showing some similarity with few acyl-CoA dehydrogenases, e.g. Q9APX7|FADE36 FADE36 protein from Pseudomonas aeruginosa (360 aa), FASTA scores: opt: 147, E(): 0.046, (26.15% identity in 214 aa overlap); part of AAB52261.2|U97002 protein similar to acyl-CoA dehydrogenases and epoxide hydrolases from Caenorhabditis elegans (985 aa), FASTA score: (31.2% identity in 324 aa overlap). C-terminal part is highly similar to Q50095|U1740AK|MLU15183_45 hypothetical protein from Mycobacterium leprae cosmid B174 (122 aa), FASTA scores: opt: 341, E(): 7.3e-15, (57.6% identity in 99 aa overlap). Contains PS00339 Aminoacyl-transfer RNA synthetases class-II signature 2. O69727 No lipid metabolism O69727 O69727 GO:0016491,GO:0055114 1.3.99.- O69727 NC_000962.3 Mycobrowser_v5 CDS 4216078 4216269 . - 0 Rv3770B Rv3770B Possibly required for the transposition of an insertion element. Probable remnant of a transposase Rv3770B, len: 63 aa. Probable remnant of a transposase, similar to many e.g. Rv2812|MTCY16B7.31c|Z81331_17 IS1604 putative transposase from Mycobacterium tuberculosis (469 aa), FASTA scores: opt: 379, E(): 1.6e-21, (93.55% identity in 62 aa overlap). Continues as Rv3770A. L7N679 No insertion seqs and phages L7N679 NC_000962.3 Mycobrowser_v5 CDS 4288260 4291529 . - 0 Rv3823c mmpL8 Thought to be involved in the transport of lipids, it has been shown to be required in the production of a sulfated glycolipid, sulfolipid-1 (SL-1). Conserved integral membrane transport protein MmpL8 Rv3823c, (MTCY409.07), len: 1089 aa. mmpL8, conserved integral membrane transport protein (see Tekaia et al., 1999), member of RND superfamily, equivalent to Q49619|MMLA_MYCLE|MMPL10|TP1|ML1231|B1170_C1_181 putative membrane protein from Mycobacterium leprae (1008 aa), FASTA scores: opt: 2718, E(): 7.3e-149, (56.25% identity in 1028 aa overlap). Also similar to others e.g. Q9XCF6|TMTPC from Mycobacterium avium (974 aa), FASTA scores: opt: 660, E(): 2.7e-30, (28.2% identity in 1050 aa overlap); Q9XCF5|TMTPB from Mycobacterium avium (963 aa), FASTA scores: opt: 653, E(): 6.7e-30, (27.0% identity in 1014 aa overlap); Q9KH53|TMTPC from Mycobacterium smegmatis (994 aa), FASTA scores: opt: 648, E(): 1.3e-29, (28.45% identity in 1013 aa overlap); etc. Also highly similar to other mmpL proteins from Mycobacterium tuberculosis; O50439|MMLA_MYCTU|MMPL10|RV1183|MT1220|MTV005.19 (1002 aa), FASTA scores: opt: 2777, E(): 2.9e-152, (58.25% identity in 996 aa overlap); Q50585|MMLC_MYCTU|MMPL12|Rv1522c|MT1573|MTCY19G5.06 (1146 aa), FASTA scores: opt: 2433, E(): 2.1e-132, (49.9% identity in 1050 aa overlap); and similar to others e.g. P95235|MML9_MYCTU|MMPL9|Rv2339|MT2402|MTCY98.08 (962 aa), FASTA scores: opt: 651, E(): 8.8e-30, (28.6% identity in 1038 aa overlap); etc. Belongs to the MmpL family. P9WJU5 No cell wall and cell processes O07800 P9WJU5 GO:0006869,GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 4356693 4357535 . + 0 Rv3878 espJ Function unknown ESX-1 secretion-associated protein EspJ. Conserved alanine rich protein. Rv3878, (MTV027.13), len: 280 aa. EspJ, ESX-1 secretion-associated protein, conserved ala-rich protein. Predicted to be an outer membrane protein (See Song et al., 2008). P9WJC3 No cell wall and cell processes P9WJC3 GO:0009405 NC_000962.3 Mycobrowser_v5 CDS 4209047 4209526 . + 0 Rv3763 lpqH Shown to inhibit gamma interferon regulated HLA-DR protein and mRNA expression in human macrophages 19 kDa lipoprotein antigen precursor LpqH Rv3763, (MTV025.111), len: 159 aa. LpqH, conserved 19 KDa lipoprotein antigen precursor (see citations below), equivalent to P31502|19KD_MYCIT|MI22 19 KDA lipoprotein antigen precursor (MI22 antigen) from Mycobacterium intracellulare (162 aa), FASTA scores: opt: 773, E(): 6.2e-35, 75.95(% identity in 162 aa overlap); P46733|19KD_MYCAV 19 KDA lipoprotein antigen precursor from Mycobacterium avium (161 aa), FASTA scores: opt: 743, E(): 2.5e-33, (72.5% identity in 160 aa overlap); and Q9X7A5|LPQH|ML1966 possible lipoprotein from Mycobacterium leprae FASTA scores: opt: 371, E(): 2.2e-13, (42.6% identity in 162 aa overlap). Possibly attached to the membrane by a lipid anchor. Similar to other mycobacterium 19 KDA antigen. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. P9WK61 No cell wall and cell processes P0A5J0 P9WK61 GO:0005886 P9WK61 NC_000962.3 Mycobrowser_v5 CDS 4306236 4306811 . - 0 Rv3832c Rv3832c Function unknown Conserved protein Rv3832c, (MTCY01A6.37), len: 191 aa. Conserved protein, similar in part to various proteins e.g. Q9XBC9|CZA382.22c putative rRNA methylase from Amycolatopsis orientalis (259 aa), FASTA scores: opt: 196, E(): 1.3e-05, (38.2% identity in 110 aa overlap); CAC48459|SMB20059 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) plasmid pSymB (259 aa), FASTA scores: opt: 188, E(): 4.3e-05, (33.8% identity in 136 aa overlap); Q98FP8|MLL3672 methyl transferase-like protein from Rhizobium loti (Mesorhizobium loti) (264 aa), FASTA scores: opt: 180, E(): 0.00014, (32.05% identity in 156 aa overlap); etc. P96246 No conserved hypotheticals P96246 P96246 GO:0008168,GO:0008152 P96246 NC_000962.3 Mycobrowser_v5 CDS 4279230 4280015 . - 0 Rv3814c Rv3814c Function unknown; probably involved in cellular metabolism. Possible acyltransferase Rv3814c, (MTCY409.16), len: 261 aa. Possible acyltransferase, highly similar to Q9CDC0|ML0087 putative acyltransferase from Mycobacterium leprae (257 aa), FASTA scores: opt: 753, E(): 7.7e-42, (46.75% identity in 246 aa overlap). Also highly similar to many acyltransferases and hypothetical proteins e.g. Q9K3R3|2SCG4.01 putative acyltransferase from Streptomyces coelicolor (242 aa), FASTA scores: opt: 587, E(): 4.6e-31, (41.95% identity in 243 aa overlap); Q9ZBS1|SC7A1.02 putative acyltransferase from Streptomyces coelicolor (264 aa), FASTA scores: opt: 293, E(): 6.6e-12, (29.2% identity in 267 aa overlap); Q9PNZ5|AAS|CJ0938 putative 2-acylglycerophosphoethanolamine acyltransferase / acyl-acyl carrier protein synthetase from Campylobacter jejuni (1170 aa), FASTA scores: opt: 274, E(): 3.9e-10, (29.1% identity in 219 aa overlap) (similarity only with middle section); Q9EY25 putative acetyl transferase from Xanthomonas oryzae pv. oryzae (249 aa), FASTA scores: opt: 238, E(): 2.4e-08, (29.2% identity in 209 aa overlap); etc. Also highly similar to downstream ORFs O07808|Rv3815c|MTCY409.15 putative acyltransferase from Mycobacterium tuberculosis (251 aa), FASTA scores: opt: 1069, E(): 2.1e-62, (60.4% identity in 245 aa overlap); and O07807|Rv3816c|MTCY409.14 putative acyltransferase from Mycobacterium tuberculosis (259 aa), FASTA scores: opt: 776, E(): 2.5e-43, (50.9% identity in 228 aa overlap). And similar to O53516|Rv2182c|MTV021.15c hypothetical 27.0 KDA protein from Mycobacterium tuberculosis (247 aa), FASTA scores: opt: 239, E(): 2e-08, (30.6% identity in 232 aa overlap). O07809 No intermediary metabolism and respiration O07809 O07809 GO:0008152,GO:0008415 2.3.1.- O07809 NC_000962.3 Mycobrowser_v5 CDS 4209582 4211009 . - 0 Rv3764c tcrY Sensor part of a two component regulatory system. Possible two component sensor kinase TcrY Rv3764c, (MTV025.112c), len: 475 aa. Possible tcrY, histidine protein kinase, part of a two-component regulatory system, similar to others e.g. Q9ADN6|2SC10A7.25 putative two component system histidine kinase from Streptomyces coelicolor (524 aa), FASTA scores: opt: 1332, E(): 5.4e-70, (49.9% identity in 477 aa overlap); Q9L3C1|KB|CAC42479 putative histidine kinase from Amycolatopsis mediterranei (469 aa), FASTA scores: opt: 515, E(): 1.4e-22, (36.1% identity in 313 aa overlap); P72560 histidine protein kinase from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (438 aa), FASTA scores: opt: 480, E(): 1.4e-20, (40.1% identity in 232 aa overlap); P30847|P76401|BAES_ECOLI|B2078 sensor protein from Escherichia coli strain K12 (467 aa); etc. Also similar to others from Mycobacterium tuberculosis e.g. P96368|Rv1032c|MTCY10G2.17 (509 aa), FASTA scores: opt: 1007, E(): 4e-51, (43.5% identity in 416 aa overlap); and P71815|Rv0758|MTCY369.03 (485 aa), FASTA scores: opt: 738, E(): 1.6e-35, (28.6% identity in 438 aa overlap). Equivalent to AAK48235 from Mycobacterium tuberculosis strain CDC1551 (506 aa) but shorter 31 aa. O69729 No regulatory proteins O69729 O69729 GO:0016021,GO:0018106,GO:0007165,GO:0000155,GO:0000160,GO:0005524 2.7.13.3 O69729 NC_000962.3 Mycobrowser_v5 CDS 4214615 4214887 . + 0 Rv3769 Rv3769 Unknown Hypothetical protein Rv3769, (MTCY13D12.03), len: 90 aa. Hypothetical unknown protein, possible coiled-coil protein. P72036 No conserved hypotheticals P72036 NC_000962.3 Mycobrowser_v5 CDS 4214070 4214429 . + 0 Rv3768 Rv3768 Unknown Unknown protein Rv3768, (MTCY13D12.02), len: 119 aa. Unknown protein. P72035 No conserved hypotheticals P72035 P72035 P72035 NC_000962.3 Mycobrowser_v5 CDS 4215200 4215775 . - 0 Rv3770c Rv3770c Unknown Hypothetical leucine rich protein Rv3770c, (MTCY13D12.04c), len: 191 aa. Hypothetical unknown leu-rich protein. P72037 No conserved hypotheticals P72037 NC_000962.3 Mycobrowser_v5 CDS 4218241 4218825 . - 0 Rv3773c Rv3773c Function unknown Conserved protein Rv3773c, (MTCY13D12.07c), len: 194 aa. Conserved protein, highly similar to C-terminal end of O53773|Rv0576|MTV039.14 possible transcriptional regulator from Mycobacterium tuberculosis (434 aa), FASTA scores: opt: 575, E(): 8.3e-30, (47.4% identity in 192 aa overlap); and some similarity with other proteins from Mycobacterium tuberculosis e.g. P71985|Rv1727|MTCY04C12.12 (189 aa) FASTA scores: opt: 176, E(): 0.00022, (31.1% identity in 180 aa overlap). P72040 No conserved hypotheticals P72040 P72040 NC_000962.3 Mycobrowser_v5 CDS 4218849 4219673 . + 0 Rv3774 echA21 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Possible enoyl-CoA hydratase EchA21 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv3774, (MTCY13D12.08), len: 274 aa. Possible echA21, enoyl-CoA hydratase, equivalent to Q9CD94|ECHA1|ML0120 putative enoyl-CoA hydratase from Mycobacterium leprae (278 aa), FASTA scores: opt: 1593, E(): 2.2e-92, (88.3% identity in 274 aa overlap). Also similar to others e.g. Q9I2S4|PA1821 from Pseudomonas aeruginosa (270 aa), FASTA scores: opt: 761, E(): 2e-40, (42.3% identity in 267 aa overlap); Q9FHR8 from Arabidopsis thaliana (Mouse-ear cress) (278 aa) FASTA scores: opt: 638, E(): 9.9e-33, (39.4% identity in 269 aa overlap); Q9AB78|CC0353 from Caulobacter crescentus (286 aa), FASTA scores: opt: 601, E(): 2.1e-31, (39.25% identity in 266 aa overlap); etc. P75019 No lipid metabolism P75019 P75019 GO:0016853,GO:0008152,GO:0004300 4.2.1.17 P75019 NC_000962.3 Mycobrowser_v5 CDS 4221089 4222648 . + 0 Rv3776 Rv3776 Function unknown Conserved hypothetical protein Rv3776, (MTCY13D12.10), len: 519 aa. Conserved hypothetical protein, highly similar to Q10709|YL00_MYCTU|Rv2100|MTCY49.40 hypothetical 58.9 KDA protein from Mycobacterium tuberculosis (550 aa) FASTA scores: opt: 1646, E(): 1.2e-83, (77.85% identity in 510 aa overlap) (homology from potential start at 7744); and similar to other proteins from Mycobacterium tuberculosis (strains H37Rv and CDC1551) e.g. O33266|Rv0336|MTCY279.03 (503 aa) FASTA scores: opt: 682, E(): 2.2e-30, (41.65% identity in 497 aa overlap). P72042 No conserved hypotheticals P72042 P72042 GO:0003676,GO:0004519 NC_000962.3 Mycobrowser_v5 CDS 4224985 4226985 . + 0 Rv3779 Rv3779 Unknown Probable conserved transmembrane protein alanine and leucine rich Rv3779, (MTCY13D12.13), len: 666 aa. Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). Probable conserved transmembrane ala-, leu-rich protein, equivalent to Q9CD98|ML0116 putative membrane protein from Mycobacterium leprae (654 aa), FASTA scores: opt: 1991, E(): 2e-112, (66.5% identity in 666 aa overlap). Shows some similarity with Q9RRU0|DR2395 putative NA+/H+ antiporter from Deinococcus radiodurans (458 aa), FASTA scores: opt: 138, E(): 0.69, (31.9% identity in 138 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P72045 No cell wall and cell processes P72045 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 4226989 4227525 . + 0 Rv3780 Rv3780 Function unknown Conserved protein Rv3780, (MTCY13D12.14), len: 178 aa. Conserved protein, equivalent to Q9CD99|ML0115 hypothetical 19.1 KDA protein from Mycobacterium leprae (174 aa), FASTA scores: opt: 903, E(): 2.3e-48, (82.95% identity in 170 aa overlap). Also highly similar to Q9XA56|SCGD3.23c hypothetical 19.5 KDA protein from Streptomyces coelicolor (179 aa), FASTA scores: opt: 692, E(): 1.8e-35, (65.9% identity in 170 aa overlap). Note that this putative protein is 4 aa longer at the N-terminus compared to previous annotation (in Nature 393: 537-544 (1998)). P9WKX3 No conserved hypotheticals P65091 P9WKX3 P9WKX3 NC_000962.3 Mycobrowser_v5 CDS 4283996 4284331 . + 0 Rv3819 Rv3819 Unknown Unknown protein Rv3819, (MTCY409.11c), len: 111 aa. Unknown protein. Contains PS00012 Phosphopantetheine attachment site. O07804 No conserved hypotheticals O07804 NC_000962.3 Mycobrowser_v5 CDS 4239863 4243147 . + 0 Rv3793 embC Involved in the biosynthesis of the mycobacterial cell wall arabinan and resistance to ethambutol (EMB; dextro-2,2'-(ethylenediimino)-DI-1-butanol). Polymerizes arabinose into the arabinan of arabiogalactan [catalytic activity: UDP-L-arabinose + indol-3-ylacetyl-myo-inositol = UDP + indol-3-ylacetyl-myo-inositol L-arabinoside]. Integral membrane indolylacetylinositol arabinosyltransferase EmbC (arabinosylindolylacetylinositol synthase) Rv3793, (MTCY13D12.27), len: 1094 aa. EmbC, integral membrane protein, indolylacetylinositol arabinosyltransferase (see citations below), equivalent to Q9CDA7|EMBC|ML0106 putative arabinosyl transferase from Mycobacterium leprae (1070 aa) FASTA scores: opt: 6078,E(): 0, (82.95% identity in 1072 aa overlap); Q50393|EMBC putative arabinosyl transferase from Mycobacterium smegmatis (1074 aa), FASTA scores: opt: 5523, E(): 0, (75.35% identity in 1072 aa overlap). Also similar to Q9CDA9|EMBB| ML0104 putative arabinosyl transferase from Mycobacterium leprae (1083 aa), FASTA scores: opt: 2789, E(): 1.9e-156, (44.0% identity in 1095 aa overlap); O30406|EMBB putative arabinosyl transferase from Mycobacterium smegmatis (1082 aa), FASTA scores: opt: 2746, E(): 6.4e-154, (44.6% identity in 1096 aa overlap); etc. Also similar to to P72030|EMBB|Rv3795|MTCY13D12.29 indolylacetylinositol arabinosyltransferase from Mycobacterium tuberculosis (1098 aa), FASTA scores: opt: 2276, E(): 3.1e-126, (44.45% identity in 1118 aa overlap); and P72060|EMBA|Rv3794|MTCY13D12.28 indolylacetylinositol arabinosyltransferase from Mycobacterium tuberculosis (1094 aa), FASTA scores: opt: 1974, E(): 1.9e-108, (41.0% identity in 1110 aa overlap). Contains PS00044 Bacterial regulatory proteins, lysR family signature; and PS00017 ATP/GTP-binding site motif A (P-loop). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WNL5 No cell wall and cell processes 3PTY P72059 P9WNL5 GO:0016021,GO:0009252,GO:0005886,GO:0046677,GO:0016763,GO:0007047 2.4.2.- EMB P9WNL5 NC_000962.3 Mycobrowser_v5 CDS 4228347 4229261 . + 0 Rv3782 glfT1 Transfers galactofuranose in the initiation of cell wall galactan polymerization UDP-galactofuranosyl transferase GlfT1 Rv3782, (MTCY13D12.16), len: 304 aa. GlfT1, UDP-galactofuranosyl transferase (See Mikusova et al., 2006; Belanova et al., 2008), equivalent to Q9CDA1|RFBE|ML0113 putative glycosyl transferase from Mycobacterium leprae (283 aa), FASTA scores: opt: 1583, E(): 9.3e-96, (81.6% identity in 277 aa overlap). Also some similarity with AAK68916|WCFN putative glycosyltransferase from Bacteroides fragilis (291 aa) FASTA scores: opt: 241, E(): 2.1e-08, (30.75% identity in 195 aa overlap); O58161|PH0424 hypothetical 40.5 KDA protein from Pyrococcus horikoshii (348 aa), FASTA scores: opt: 194, E(): 2.8e-05, (23.85% identity in 302 aa overlap); O26448|MTH348 rhamnosyl transferase from Methanothermobacter thermautotrophicus (313 aa), FASTA scores: opt: 177, E(): 0.00033, (28.2% identity in 333 aa overlap); O07868|CPS19BQ putative rhamnosyl transferase FASTA from Streptococcus pneumoniae (300 aa), FASTA scores: opt: 156, E(): 0.0074, (25.45% identity in 232 aa overlap); and other putative transferases. Note that C-terminal end shows some similarity with part of Q05161|RFB O-antigen biosynthesis protein B from Escherichia coli strain 0101. Note that previously known as rfbE. P9WMX3 No cell wall and cell processes Q7D4V6 P9WMX3 GO:0016757 2.4.1.- P9WMX3 NC_000962.3 Mycobrowser_v5 CDS 4229258 4230100 . + 0 Rv3783 rfbD May form an ATP-driven O-antigen/lipopolysaccharide export apparatus, in association with RFBE|Rv3781. Responsible for the translocation of the substrate across the membrane. Probable O-antigen/lipopolysaccharide transport integral membrane protein ABC transporter RfbD Rv3783, (MTCY13D12.17), len: 280 aa. Probable rfbD, polysaccharide-transport integral membrane protein ABC transporter (see Braibant et al., 2000), involved in O-antigen/lipopolysaccharides (LPS) transport, equivalent to Q9CDA2|ML0112 putative ABC transporter component from Mycobacterium leprae (276 aa), FASTA scores: opt: 1646, E(): 4e-102, (84.3% identity in 280 aa overlap). Also highly similar to Q9PAF1|XF2567 ABC transporter permease protein from Xylella fastidiosa (267 aa), FASTA scores: opt: 723, E(): 7.6e-41, (41.3% identity in 259 aa overlap); and similar to others e.g. Q56902|RFBD_YEREN O-antigen export system permease protein from Yersinia enterocolitica (259 aa) (see Zhang et al., 1993), FASTA scores: opt: 566, E(): 2e-30, (28.05% identity in 264 aa overlap); Q06955|RFBH RFBH protein (involved in the export of lipopolysaccharide) (alias Q9KVA3|VC0246) lipopolysaccharide/O-antigen transport protein from Vibrio cholerae (257 aa), FASTA scores: opt: 358, E(): 1.3e-16, (24.4% identity in 258 aa overlap); Q9HTB8|WZM|PA5451 membrane subunit of a-band LPS efflux transporter from Pseudomonas aeruginosa (265 aa), FASTA scores: opt: 263, E(): 2.7e-10, (25.45% identity in 263 aa overlap); etc. Belongs to the ABC-2 subfamily of integral membrane proteins. P72049 No cell wall and cell processes P72049 P72049 GO:0016020 P72049 NC_000962.3 Mycobrowser_v5 CDS 4231320 4232393 . + 0 Rv3785 Rv3785 Unknown Hypothetical protein Rv3785, (MTCY13D12.19), len: 357 aa. Hypothetical unknown protein. Note that this putative protein is equivalent to AAK48258|MT3893 NAD-dependent epimerase/dehydratase family protein from Mycobacterium tuberculosis strain CDC1551 (712 aa), but shorter 355 aa. P9WKX1 No conserved hypotheticals P9WKX1 P9WKX1 NC_000962.3 Mycobrowser_v5 CDS 4232374 4233597 . - 0 Rv3786c Rv3786c Unknown Unknown protein Rv3786c, (MTCY13D12.20), len: 407 aa. Unknown protein. Segment between aa 265-300 (approximately) is highly similar to part of O03937|RORF1608 minor capsid protein from Bacteriophage phig1e (1608 aa), FASTA scores: opt: 242, E(): 8.4e-07, (26.85% identity in 272 aa overlap); Q9ETT9|ORF36 putative peptidase from Corynebacterium equii (Rhodococcus equi) plasmid pREAT701 (p33701) and Plasmid virulence (546 aa), FASTA scores: opt: 231, E(): 1.6e-06, (34.15% identity in 167 aa overlap); O69910|SC2E1.40c hypothetical 22.8 KDA protein. from Streptomyces coelicolor (226 aa) FASTA scores: opt: 218, E(): 4.6e-06, (34.15% identity in 164 aa overlap); and others. P9WKW9 No conserved hypotheticals P0A5H1 P9WKW9 GO:0006508,GO:0004222 P9WKW9 NC_000962.3 Mycobrowser_v5 CDS 4233610 4234536 . - 0 Rv3787c Rv3787c Function unknown Conserved hypothetical protein Rv3787c, (MTCY13D12.21), len: 308 aa. Conserved hypothetical protein, highly similar to several mycobacterial hypothetical proteins e.g. P95074|Rv0726c|MTCY210.45c from Mycobacterium tuberculosis (367 aa), FASTA scores: opt: 1038, E(): 1.6e-58, (55.85% identity in 283 aa overlap); O53795|MBE50c|Rv0731c|MTV041.05c from Mycobacterium tuberculosis (318 aa), FASTA scores: opt: 1030, E(): 4.5e-58, (56.15% identity in 292 aa overlap); Q9CCZ4|ML2640 from Mycobacterium leprae (310 aa) FASTA scores: opt: 709, E(): 9.9e-38, (43.75% identity in 279 aa overlap); etc. P9WFH3 No conserved hypotheticals P72053 P9WFH3 GO:0008168 2.1.1.- P9WFH3 NC_000962.3 Mycobrowser_v5 CDS 4234780 4235265 . + 0 Rv3788 Rv3788 Unknown Hypothetical protein Rv3788, (MTCY13D12.22), len: 161 aa. Hypothetical unknown protein. P9WKW7 No conserved hypotheticals P65095 P9WKW7 GO:0006355,GO:0003711,GO:0003677 P9WKW7 NC_000962.3 Mycobrowser_v5 CDS 4235374 4235739 . + 0 Rv3789 Rv3789 Function unknown GTRA family protein Rv3789, (MTCY13D12.23), len: 121 aa. GtrA family protein; possible integral membrane protein, equivalent to Q9CDA3|ML0110 hypothetical 13.9 KDA protein from Mycobacterium leprae (123 aa) FASTA scores: opt: 587, E(): 7.3e-34, (72.95% identity in 122 aa overlap). Also equivalent to AAK48262 from Mycobacterium tuberculosis strain CDC1551 (142 aa) but shorter 21 aa. P9WMS9 No cell wall and cell processes P64292 P9WMS9 GO:0005886,GO:0000271,GO:0006810,GO:0016021 P9WMS9 NC_000962.3 Mycobrowser_v5 CDS 4246514 4249810 . + 0 Rv3795 embB Involved in the biosynthesis of the mycobacterial cell wall arabinan and resistance to ethambutol (EMB; dextro-2,2'-(ethylenediimino)-DI-1-butanol). Polymerizes arabinose into the arabinan of arabiogalactan [catalytic activity: UDP-L-arabinose + indol-3-ylacetyl-myo-inositol = UDP + indol-3-ylacetyl-myo-inositol L-arabinoside]. Integral membrane indolylacetylinositol arabinosyltransferase EmbB (arabinosylindolylacetylinositol synthase) Rv3795, (MTCY13D12.29), len: 1098 aa. EmbB, integral membrane protein, indolylacetylinositol arabinosyltransferase (see citations below), equivalent to P71486|EMBB arabinosyl transferase from Mycobacterium avium (1065 aa), FASTA scores: opt: 4998, E(): 0, (83.25% identity in 1076 aa overlap); Q9CDA9|EMBB|ML0104 putative arabinosyl transferase from Mycobacterium leprae (1083 aa), FASTA scores: opt: 4706, E(): 0, (78.0% identity in 1101 aa overlap); O30406|EMBB (alias Q50395) putative arabinosyl transferase from Mycobacterium smegmatis (1082 aa), FASTA scores: opt: 4163, E(): 0, (68.4% identity in 1091 aa overlap); etc. Also similar to Q50393|EMBC putative arabinosyl transferase from Mycobacterium smegmatis (1074 aa), FASTA scores: opt: 2482, E(): 5e-135, (44.7% identity in 1101 aa overlap); Q9CDA7|EMBC|ML0106 putative arabinosyl transferase from Mycobacterium leprae (1070 aa), FASTA scores: opt: 2259, E(): 3.4e-122, (43.4% identity in 1104 aa overlap); etc. Also similar to P72059|EMBC|Rv3793|MTCY13D12.27 indolylacetylinositol arabinosyltransferase from Mycobacterium tuberculosis (1094 aa), FASTA scores: opt: 2276, E(): 3.6e-123, (44.45% identity in 1118 aa overlap); and P72060|EMBA|Rv3794|MTCY13D12.28 indolylacetylinositol arabinosyltransferase from Mycobacterium tuberculosis (1094 aa), FASTA scores: opt: 1288, E(): 2.5e-66, (42.35% identity in 1114 aa overlap). Supposedly regulated by embR|Rv1267c. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WNL7 No cell wall and cell processes P72030 P9WNL7 GO:0016021,GO:0009252,GO:0005886,GO:0046677,GO:0016763,GO:0007047 2.4.2.- EMB,INH,RIF P9WNL7 NC_000962.3 Mycobrowser_v5 CDS 4249878 4251005 . + 0 Rv3796 Rv3796 Function unknown Conserved protein Rv3796, (MTV026.01-MTCY13D12.30), len: 375 aa. Conserved protein. C-terminal end similar in part to Q983J3|MLR8305 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (227 aa), FASTA scores: opt: 288, E(): 4e-09, (38.95% identity in 154 aa overlap). Similar to P54548|YQJK_BACSU hypothetical protein (belongs to the ATSA/ELAC family) from Bacillus subtilis (307 aa) FASTA scores: opt: 263, E(): 1.3e-07, (26.1% identity in 295 aa overlap); and some similarity to other proteins e.g. AAK46775|MT2479 putative arylsulfatase from Mycobacterium tuberculosis strain CDC1551 (224 aa), FASTA scores: opt: 194, E(): 0.00072, (25.85% identity in 259 aa overlap). Equivalent to AAK48269 from Mycobacterium tuberculosis strain CDC1551 (338 aa) but longer 37 aa. Some similarity to the A. carrageenovora AtsA / E. coli ElaC family. Note that previously known as atsH. Predicted to be an outer membrane protein (See Song et al., 2008). P72062 No conserved hypotheticals P72062 P72062 NC_000962.3 Mycobrowser_v5 CDS 4270366 4272279 . - 0 Rv3808c glfT2 Converts UDP-galactofuranose in cell wall galactan polymerization. Has UDP-Galf:beta-D-(1->5) and UDP-Galf:beta-D-(1->6) galactofuranosyltransferase activities. Bifunctional UDP-galactofuranosyl transferase GlfT2 Rv3808c, (MTV026.13c), len: 637 aa. GlfT2, bifunctional UDP-galactofuranosyl transferase (see citations below). Equivalent to Q9CDB7|ML0093 hypothetical protein from Mycobacterium leprae (643 aa), FASTA scores: opt: 3751, E(): 0, (85.4% identity in 643 aa overlap). Contains a beta-glycosyltransferase domain A. Note that previously known as glfT. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53585 No cell wall and cell processes O53585 GO:0016740 2.4.1.- O53585 NC_000962.3 Mycobrowser_v5 CDS 4252993 4254327 . + 0 Rv3798 Rv3798 Required for the transposition of the insertion sequence element IS1557. Probable transposase Rv3798, (MTV026.03), len: 444 aa. Probable transposase for insertion sequence element IS1557, highly similar to Q60255 similar to transposase of ISAE1 from alcaligenes eutrophus H1-4 (fragment) from dibenzofuran-degrading bacterium DPO360 (163 aa) FASTA scores: opt: 767, E(): 3.2e-42, (67.25% identity in 168 aa overlap); and similar to P74920 transposase from Thiobacillus ferrooxidans (404 aa), FASTA scores: opt: 375, E(): 1.1e-16, (27.55% identity in 439 aa overlap); Q48349 transposase from Alcaligenes eutrophus (Ralstonia eutropha) (408 aa), FASTA scores: opt: 324, E(): 2e-13, (3.9% identity in 369 aa overlap); Q9FDC1|TNP transposase from Burkholderia mallei (Pseudomonas mallei) (386 aa) FASTA scores: opt: 282, E(): 9.8e-11, (25.85% identity in 391 aa overlap); etc. C-terminal end identical to O53804|Rv0741|MTV041.15 transposase from Mycobacterium tuberculosis (104 aa), FASTA scores: opt: 582, E(): 1.8e-30, (85.6% identity in 104 aa overlap). Belongs to the transposase family 12. P9WKH7 No insertion seqs and phages Q10621 P9WKH7 GO:0004803,GO:0006313,GO:0003677 P9WKH7 NC_000962.3 Mycobrowser_v5 CDS 4255945 4261146 . - 0 Rv3800c pks13 Involved in the final steps of mycolic acid biosynthesis. Catalyses the condensation of two fatty acyl chains. Polyketide synthase Pks13 Rv3800c, (MTV026.05c), len: 1733 aa. Probable pks13, polyketide synthase, equivalent to Q9CDB1|PKS13|ML0101 polyketide synthase from Mycobacterium leprae (1784 aa), FASTA scores: opt: 7454, E(): 0, (83.6% identity in 1748 aa overlap); and similar to Q9Z5K6|ML2357|MLCB12.02c putative polyketide synthase from Mycobacterium leprae (1871 aa), FASTA scores: opt: 1682, E(): 1.2e-85, (38.3% identity in 1096 aa overlap). Also similar in part to many e.g. Q9ADL6|SORA soraphen polyketide synthase a from Polyangium cellulosum (6315 aa) FASTA scores: opt: 1422, E(): 1e-70, (31.45% identity in 1616 aa overlap); AAK73501|AMPHI AMPHI protein (involved in amphotericin biosynthesis) from Streptomyces nodosus (9510 aa), FASTA scores: opt: 1441, E(): 1.2e-71, (30.45% identity in 1662 aa overlap); Q9RFL0|MTAB MTAB protein (involved in myxothiazol biosynthesis) from Stigmatella aurantiaca (4003 aa), FASTA scores: opt: 1429, E(): 2.8e-71, (33.8% identity in 1089 aa overlap); Q9L4X2|NYSJ from Streptomyces noursei (5435 aa), FASTA scores: opt: 1407, E(): 6.1e-70, (30.5% identity in 1764 aa overlap); CAC37876|SC1G7.01c from Streptomyces coelicolor (3489 aa) FASTA scores: opt: 1382, E(): 1e-68, (31.05% identity in 1489 aa overlap); etc. Also highly similar to Q10977|PPSA_MYCTU|Rv2931|MT3000|MTCY338.20 phenolpthiocerol synthesis polyketide synthase from Mycobacterium tuberculosis (1876 aa), FASTA scores: opt: 1728, E(): 3.4e-88, (36.95% identity in 1269 aa overlap); and P96203|PPSD|Rv2934|MTCY19H9.02. Contains PS00606 Beta-ketoacyl synthases active site. I6X8D2 No lipid metabolism O53579 I6X8D2 GO:0009058,GO:0048037,GO:0016788,GO:0016740,GO:0000036 I6X8D2 NC_000962.3 Mycobrowser_v5 CDS 4266953 4268836 . - 0 Rv3805c aftB Involved in the biosynthesis of the mycobacterial cell wall arabinan Possible arabinofuranosyltransferase AftB Rv3805c, (MTV026.10c), len: 627 aa. Possible aftB, arabinofuranosyltransferase (See Seidel et al., 2007). Probable conserved transmembrane protein, equivalent, but shorter 19 aa, to Q9CDB4|ML0096 putative membrane protein from Mycobacterium leprae (649 aa), FASTA scores: opt: 3511, E(): 1.1e-204, (80.9% identity in 629 aa overlap). Equivalent to AAK48278 from Mycobacterium tuberculosis strain CDC1551 (641 aa) but shorter 14 aa. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53582 No cell wall and cell processes O53582 GO:0016021 O53582 NC_000962.3 Mycobrowser_v5 CDS 4263355 4264365 . - 0 Rv3802c Rv3802c Shown to have phospholipase and thioesterase activity Probable conserved membrane protein Rv3802c, (MTV026.07c), len: 336 aa. Probable conserved membrane protein, with a N-terminal signal sequence followed by Pro-rich region. Equivalent to Q9CDB3|ML0099 hypothetical protein from Mycobacterium leprae (336 aa) FASTA scores: opt: 1759, E(): 1.1e-85, (75.5% identity in 335 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). O53581 No cell wall and cell processes O53581 O53581 GO:0008152,GO:0016787 O53581 NC_000962.3 Mycobrowser_v5 CDS 4264563 4265462 . - 0 Rv3803c fbpD Involved in cell wall mycoloylation. Proteins of the antigen 85 complex are responsible for the high affinity of mycobacteria to fibronectin. Possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity. Secreted MPT51/MPB51 antigen protein FbpD (MPT51/MPB51 antigen 85 complex C) (AG58C) (mycolyl transferase 85C) (fibronectin-binding protein C) (85C) Rv3803c, (MT3910, MTV026.08c), len: 299 aa. FbpD (alternate gene names: mpt51, mpb51, fbpC1), secreted MPB51/MPT51 antigen protein (fibronectin-binding protein C) (mycolyl transferase 85C) (see citations below), identical to Q48923|MPT51|MPB51 antigen precursor from Mycobacterium bovis (299 aa), FASTA scores: opt: 2093, E(): 1.5e-112, (100.0% identity in 299 aa overlap) (see Ohara et al., 1995); and highly similar to other Mycobacterial antigen precursors e.g. Q05868|MPT5_MYCLE|MPT51|ML0098 MPT51 antigen precursor from Mycobacterium leprae (301 aa), FASTA scores: opt: 1624, E(): 9.8e-86, (77.8% identity in 302 aa overlap); O52972|A85C_MYCAV|FBPC antigen 85-C precursor (fibronectin-binding protein C) from Mycobacterium avium (352 aa), FASTA scores: opt: 753, E(): 6.6e-36, (41.5% identity in 315 aa overlap); P21160|A85B_MYCKA antigen 85-B precursor (fibronectin-binding protein B) from Mycobacterium kansasii (325 aa), FASTA scores: opt: 574, E(): 1.1e-25, (37.55% identity in 309 aa overlap); P12942|A85B_MYCBO antigen 85-B precursor from Mycobacterium bovis (323 aa), FASTA scores: opt: 572, E(): 1.4e-25, (39.85% identity in 291 aa overlap); etc. Also similar to P31953|A85C_MYCTU|FBPC|MPT45|Rv0129c|MTCI5.03c|FBPC2 secreted antigen 85-C (mycolyl transferase 85C) (fibronectin-binding protein C) from Mycobacterium tuberculosis (340 aa), FASTA scores: opt: 751, E(): 8.4e-36, (40.65% identity in 310 aa overlap); P17944|A85A_MYCTU|FBPA|MPT44|Rv3804c|MT3911|MTV026.09c secreted antigen 85-a (mycolyl transferase 85A) (fibronectin-binding protein A) from Mycobacterium tuberculosis (338 aa), FASTA scores: opt: 592, E(): 1e-26, (39.05% identity in 302 aa overlap); etc. Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Note that the secreted protein MPB51 is one of the major proteins in the culture filtrate of Mycobacterium bovis BCG. Note that overexpression in an FbpC-deficient M. tuberculosis clinical isolate has no effect on the amount of cell wall-linked mycolates (See Puech et al., 2002). P9WQN7 No lipid metabolism 1R88 P0A4V6 P9WQN7 GO:0005576,GO:0008415 P9WQN7 NC_000962.3 Mycobrowser_v5 CDS 4276571 4278085 . + 0 Rv3812 PE_PGRS62 Function unknown. Thought to be involved in virulence. PE-PGRS family protein PE_PGRS62 Rv3812, (MTV026.17, MTCY409.18c), len: 504 aa. PE_PGRS62, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citations below), similar to many e.g. P96828|Rv0151c|MTCI5.25c (588 aa), FASTA scores: opt: 389, E(): 6.2e-14, (29.2% identity in 473 aa overlap); MTCY7H7B_27; MTCY493_24; MTCY441_4; MTCY39_36; MTCY1A11_4; MTCY359_33; MTCY130_10; MTCY98_9; etc. The transcription of this CDS seems to be activated in macrophages (see Ramakrishnan et al., 2000). L7N680 No PE/PPE Q7D4U2 L7N680 L7N680 NC_000962.3 Mycobrowser_v5 CDS 4265642 4266658 . - 0 Rv3804c fbpA Involved in cell wall mycoloylation. Proteins of the antigen 85 complex are responsible for the high affinity of mycobacteria to fibronectin. Possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity. Secreted antigen 85-a FbpA (mycolyl transferase 85A) (fibronectin-binding protein A) (antigen 85 complex A) Rv3804c, (MT3911, MTV026.09c), len: 338 aa. FbpA (alternate gene names: mpt44, 85A), precursor of the 85-a antigen (fibronectin-binding protein A) (mycolyl transferase 85A) (see citations below), identical to P17944|P17996|FBPA|MPT44 antigen 85-a precursor from Mycobacterium bovis (338 aa), FASTA scores: opt: 2341, E(): 1.2e-132, (100.0% identity in 338 aa overlap); and highly similar to other Mycobacterial antigen precursors e.g. O52956|A85A_MYCAV|FBPA antigen 85-a precursor (85A) from Mycobacterium avium (347 aa), FASTA scores: opt: 1987, E(): 1.7e-111, (82.55% identity in 338 aa overlap); Q05861|A85A_MYCLE|FBPA|ML0097 antigen 85-a precursor (85A) from Mycobacterium leprae (330 aa), FASTA scores: opt: 1936, E(): 1.9e-108, (83.0% identity in 329 aa overlap); O06052|A85A_MYCGO|FBPA antigen 85-a precursor (85A) from Mycobacterium gordonae (339 aa), FASTA scores: opt: 1932, E(): 3.3e-108, (80.45% identity in 338 aa overlap); etc. Also highly similar to P31952|A85B_MYCTU|FBPB|Rv1886c|MT1934|MTCY180.32 secreted antigen 85-B from Mycobacterium tuberculosis (325 aa), FASTA scores: opt: 1830, E(): 3.9e-102, (78.85% identity in 317 aa overlap); P31953|A85C_MYCTU|FBPC|MPT45|Rv0129c|MTCI5.03c|FBPC2 secreted antigen 85-C from Mycobacterium tuberculosis (340 aa), FASTA scores: opt: 1597, E(): 3.4e-88, (67.25% identity in 336 aa overlap). Predicted possible vaccine candidate (See Zvi et al., 2008). P9WQP3 No lipid metabolism 1SFR P0A4V2 P9WQP3 GO:0005576,GO:0008415 2.3.1.- P9WQP3 NC_000962.3 Mycobrowser_v5 CDS 4302786 4303397 . - 0 Rv3828c Rv3828c Prevents the cointegration of foreign DNA before integration into the chromosome. Possible resolvase Rv3828c, (MTCY409.02), len: 203 aa. Possible resolvase within IS1537 element, similar to others e.g. Q97X40|SSO1915 first ORF in transposon ISC1913 from Sulfolobus solfataricus (213 aa), FASTA scores: opt: 275, E(): 1.6e-11, (30.6% identity in 196 aa overlap); Q9V1M0|PAB2076 resolvase related protein from Pyrococcus abyssi (212 aa), FASTA scores: opt: 254, E(): 4.2e-10, (29.95% identity in 197 aa overlap); Q9RMU7|ORFA putative transposase (belongs to the MerR family of transcriptional regulators) from Helicobacter pylori (Campylobacter pylori) (217 aa), FASTA scores: opt: 243, E(): 2.3e-09, (31.8% identity in 154 aa overlap); etc. Also highly similar to proteins from Mycobacterium tuberculosis e.g. O33334|Rv2792c|MTV002.57c resolvase (193 aa), FASTA scores: opt: 970, E(): 1.5e-58, (79.25% identity in 193 aa overlap); O07773|Rv0605|MTCY19H5.17c putative resolvase (202 aa), FASTA scores: opt: 964, E(): 4e-58, (76.25% identity in 202 aa overlap); P95116|Rv2979c|MTCY349.08 hypothetical 21.4 KDA protein (194 aa), FASTA scores: opt: 895, E(): 1.8e-53, (74.75% identity in 194 aa overlap); Q10831|YS86_MYCTU|Rv2886c|MT2954|MTCY274.17c hypothetical 31.9 KDA protein (295 aa), FASTA scores: opt: 826, E(): 1.1e-48, (66.2% identity in 204 aa overlap) (similarity only at C-terminus); etc. Contains PS00397 Site-specific recombinases active site. Possible helix-turn-helix motif from aa 11-32, Score 1305 (+3.63 SD). O07795 No insertion seqs and phages O07795 O07795 GO:0006310,GO:0000150,GO:0003677 O07795 NC_000962.3 Mycobrowser_v5 CDS 4269840 4270337 . - 0 Rv3807c Rv3807c Unknown Possible conserved transmembrane protein Rv3807c, (MTV026.12), len: 165 aa. Possible conserved transmembrane protein, equivalent to Q9CDB6|ML0094 putative membrane protein from Mycobacterium leprae (192 aa), FASTA scores: opt: 714, E(): 2.4e-38, (72.85% identity in 151 aa overlap). Also highly similar to Q9KZA3|SC5G8.11 putative integral membrane protein from Streptomyces coelicolor (169 aa), FASTA scores: opt: 324, E(): 1.1e-13, (41.5% identity in 159 aa overlap); and similar in part to others e.g. Q9K3L3|SCG20A.27 putative integral membrane protein from Streptomyces coelicolor (230 aa), FASTA scores: opt: 277, E(): 1.3e-10, (41.65% identity in 168 aa overlap); P72269|ORF8 hypothetical protein from Rhodococcus erythropolis (487 aa) FASTA scores: opt: 229, E(): 2.7e-07, (36.25% identity in 149 aa overlap); O86625|SC3A7.24c putative integral membrane protein from Streptomyces coelicolor (201 aa) FASTA scores: opt: 200, E(): 9.1e-06, (34.95% identity in 146 aa overlap); Q9KYD7|SCD72A.19 putative integral membrane protein from Streptomyces coelicolor (238 aa) FASTA scores: opt: 178, E(): 0.00026, (35.7% identity in 112 aa overlap); etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WI53 No cell wall and cell processes O53584 P9WI53 GO:0016021,GO:0003824 P9WI53 NC_000962.3 Mycobrowser_v5 CDS 4321538 4322071 . + 0 Rv3847 Rv3847 Unknown Hypothetical protein Rv3847, (MTCY01A6.21c), len: 177 aa. Conserved hypothetical protein, equivalent to Q9CDC7|ML0071 hypothetical protein from Mycobacterium leprae (177 aa) FASTA scores: opt: 1149, E(): 1.6e-64, (96.6% identity in 177 aa overlap); and Q9F9R0 hypothetical 18.5 KDA protein from Mycobacterium paratuberculosis (177 aa), FASTA scores: opt: 1139, E(): 6.8e-64, (96.6% identity in 177 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P96230 No conserved hypotheticals P96230 NC_000962.3 Mycobrowser_v5 CDS 4286721 4287935 . + 0 Rv3822 Rv3822 Function unknown Conserved hypothetical protein Rv3822, (MTCY409.08c), len: 404 aa. Conserved hypothetical protein, similar in part to hypothetical proteins from Mycobacterium leprae: Q9CC62|ML1232 (358 aa) FASTA scores: opt: 601, E(): 1.1e-25, (36.7% identity in 335 aa overlap); and Q49633|B1170_F3_112 (391 aa) FASTA scores: opt: 601, E(): 1.2e-25, (36.25% identity in 347 aa overlap). Also similar to P71862|Rv3539|MTCY03C7.17c PPE family protein from Mycobacterium tuberculosis (479 aa), FASTA scores: opt: 547, E(): 1.3e-22, (38.1% identity in 281 aa overlap); O50440|Rv1184c|MTV005.20c (359 aa); O06828|Rv1430|MTCY493.24c (528 aa); O53642|Rv0159c|MTV032.02c (468 aa); etc. O07801 No conserved hypotheticals O07801 O07801 O07801 NC_000962.3 Mycobrowser_v5 CDS 4309047 4310396 . + 0 Rv3835 Rv3835 Unknown Conserved membrane protein Rv3835, (MTCY01A6.34c), len: 449 aa. Conserved membrane protein, equivalent to Q9CDC2|ML0081 putative membrane protein from Mycobacterium leprae (450 aa), FASTA scores: opt: 2079, E(): 1.8e-74, (69.35% identity in 457 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WKW5 No cell wall and cell processes P9WKW5 GO:0016021,GO:0005886,GO:0005576 NC_000962.3 Mycobrowser_v5 CDS 4385373 4386308 . - 0 Rv3900c Rv3900c Function unknown Conserved hypothetical alanine rich protein Rv3900c, (MTCY15F10.12), len: 311 aa. Conserved hypothetical ala-rich protein, highly similar to N-terminal end of Q10690|YK82_MYCTU|Rv2082|MTCY49.21 hypothetical 73.6 KDA protein from Mycobacterium tuberculosis (721 aa), FASTA scores: opt: 592, E(): 2.7e-22, (37.15% identity in 280 aa overlap). Note that MTCY15F10.12 and MTCY15F10.13 appear frameshifted with respect to MTCY49.21 although the sequence appears to be correct. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O05445 No conserved hypotheticals O05445 O05445 NC_000962.3 Mycobrowser_v5 CDS 4281647 4282402 . + 0 Rv3817 Rv3817 Function unknown; probably involved in cellular metabolism [catalytic activity: ATP + substrate = ADP + substrate 3'-phosphate]. Possible phosphotransferase Rv3817, (MTCY409.13c), len: 251 aa. Possible phosphotransferase, similar to many phosphotransferases e.g. O53023 kanamycin marker from Escherichia coli (264 aa), FASTA scores: opt: 232, E(): 7.5e-08, (32.4% identity in 247 aa overlap); BAA78209|NEO neomycine phosphotransferase from Drosophila melanogaster (Fruit fly) (264 aa), FASTA scores: opt: 227, E(): 1.6e-07, (32.0% identity in 247 aa overlap); AAG09774 aminoglycoside 3'-phosphotransferase from Vibrio cholerae (264 aa), FASTA scores: opt: 227, E(): 1.6e-07, (32.0% identity in 247 aa overlap); P00552|KKA2_KLEPN|NEO|KAN aminoglycoside 3'-phosphotransferase from Klebsiella pneumoniae (264 aa), FASTA scores: opt: 227, E(): 1.6e-07, (32.0% identity in 247 aa overlap); etc. O07806 No intermediary metabolism and respiration O07806 O07806 GO:0008910 2.7.1.95 O07806 NC_000962.3 Mycobrowser_v5 CDS 4284419 4285825 . - 0 Rv3820c papA2 Involved in sulfolipid-1 (SL-1) biosynthesis Possible conserved polyketide synthase associated protein PapA2 Rv3820c, (MTCY409.10), len: 468 aa. Possible papA2, conserved polyketide synthase (PKS) associated protein, highly similar to Q49618|PAPA3|ML1230|B1170_C1_180 PKS-associated protein A3 from Mycobacterium leprae (471 aa), FASTA scores: opt: 1660, E(): 2.7e-102, (53.95% identity in 456 aa overlap). Also similar to Q9F2R3|SCD65.19c hypothetical 52.8 KDA protein from Streptomyces coelicolor (473 aa), FASTA scores: opt: 575, E(): 1.8e-30, (27.8% identity in 464 aa overlap); and weakly similar to part of other proteins. Also high similarity with other PKS-associated proteins from Mycobacterium tuberculosis; O50438|PAPA3|Rv1182|MTV005.18 (472 aa), FASTA scores: opt: 1694, E(): 1.5e-104, (53.8% identity in 461 aa overlap); and O07799|PAPA1|Rv3824c|MTCY409.06 (511 aa), FASTA scores: opt: 1664, E(): 1.6e-102, (53.9% identity in 462 aa overlap); and similar to C-terminal end of O53902|PAPA4|Rv1528c|MTV045.02 (165 aa), FASTA scores: opt: 186, E(): 4.1e-05, (37.9% identity in 66 aa overlap). P9WIK7 No lipid metabolism Q7D4T4 P9WIK7 GO:0008415 2.3.1.- P9WIK7 NC_000962.3 Mycobrowser_v5 CDS 4285973 4286686 . + 0 Rv3821 Rv3821 Unknown Probable conserved integral membrane protein Rv3821, (MTCY409.09c), len: 237 aa. Probable conserved integral membrane protein, equivalent to Q49630|ML1233|B1170_F2_64 hypothetical 24.4 KDA protein/INTEGRAL MEMBRANE PROTEIN (POTENTIAL) from Mycobacterium leprae (230 aa), FASTA scores: opt: 619, E(): 2.4e-32, (46.65% identity in 240 aa overlap). Shows some similarity to P29466|I1BC_HUMAN|CASP1|IL1BC|IL1BCE (404 aa), FASTA scores: opt: 126, E(): 0.88, (29.05% identity in 155 aa overlap). Also highly similar to P71796|Rv1517|MTCY277.39 HYPOTHETICAL 26.9 KDA PROTEIN from Mycobacterium tuberculosis (254 aa), FASTA scores: opt: 284, E(): 5.4e-11, (36.35% identity in 256 aa overlap). Start site chosen on basis of similarity to LEPB1170_F2_64 and MTCY277.39, but may extend further upstream. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O07802 No cell wall and cell processes O07802 O07802 NC_000962.3 Mycobrowser_v5 CDS 4323499 4323897 . + 0 Rv3849 espR Involved in transcriptional mechanism. Regulates transcription of genes required for ESX-1 system. ESX-1 transcriptional regulatory protein EspR Rv3849, (MTCY01A6.19c), len: 132 aa. EspR, ESX-1 secreted protein regulator (See Raghavan et al., 2008), equivalent to Q9CDC9|ML0069 hypothetical protein from Mycobacterium leprae (132 aa) FASTA scores: opt: 724, E(): 8.7e-41, (83.95% identity in 131 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WJB7 No regulatory proteins 3QF3,3QWG,3QYX,3R1F P9WJB7 GO:0005576,GO:0009405,GO:0045449,GO:0006350,GO:0003677 P9WJB7 NC_000962.3 Mycobrowser_v5 CDS 4293225 4299605 . - 0 Rv3825c pks2 Function unknown; supposedly involved in lipid metabolism. Polyketide synthase Pks2 Rv3825c, (MTCY409.05), len: 2126 aa. pks2, polyketide synthase (see citation below), equivalent to Q9CD78|mas|ML0139 putative mycocerosic synthase from Mycobacterium leprae (2116 aa), FASTA scores: opt: 6828, E(): 0, (63.3% identity in 2128 aa overlap); and Q49624|PKS3|MASA|ML1229|B1170_C2_209 probable mycocerosic acid synthase from Mycobacterium leprae (2118 aa) FASTA scores: opt: 5220, E(): 0, (62.4% identity in 2130 aa overlap); or similar in part to others from Mycobacterium leprae e.g. Q9CB70|ML2354 polyketide synthase (1822 aa) FASTA scores: opt: 2787, E(): 2.1e-145, (34.7% identity in 2135 aa overlap). Also highly similar to Q02251|MCAS_MYCBO|mas mycocerosic acid synthase from Mycobacterium bovis (2110 aa), FASTA scores: opt: 3495, E(): 2.6e-184, (61.65% identity in 2130 aa overlap). Also highly similar to other polyketide synthases from Mycobacterium tuberculosis e.g. O53901|PKS5|Rv1527c|MTV045.01c|MTCY19G5.01 (2108 aa) FASTA scores: opt: 9576, E(): 0, (69.8% identity in 2124 aa overlap); P96291|mas|Rv2940c|MTCY24G1.09|MTCY19H9.08c (2111 aa), FASTA scores: opt: 3518, E(): 1.4e-185, (64.05% identity in 2126 aa overlap); O50437|PKS4|Rv1181|MTV005.17 (1582 aa), FASTA scores: opt: 3461, E(): 1.6e-182, (64.55% identity in 1609 aa overlap); etc. Contains PS00606 Beta-ketoacyl synthases active site and PS00012 Phosphopantetheine attachment site. P9WQE9 No lipid metabolism O07798 P9WQE9 GO:0008415,GO:0048037,GO:0006633,GO:0055114,GO:0016491,GO:0031177,GO:0008270,GO:0000036 2.3.1.- P9WQE9 NC_000962.3 Mycobrowser_v5 CDS 4299812 4301566 . + 0 Rv3826 fadD23 Function unknown, but involved in lipid degradation. Probable fatty-acid-AMP ligase FadD23 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) Rv3826, (MTCY409.04c), len: 584 aa. Probable fadD23, fatty-acid-AMP synthetase, highly similar to P71495 acyl-CoA synthase from Mycobacterium bovis (582 aa), FASTA scores: opt: 2571, E(): 4.4e-146, (66.15% identity in 576 aa overlap); Q9CD79|FADD28|ML0138 acyl-CoA synthetase from Mycobacterium leprae (579 aa) FASTA scores: opt: 2520, E(): 4.9e-143, (65.2% identity in 575 aa overlap); P54200|FD21_MYCLE putative fatty-acid--CoA ligase (acyl-CoA synthetase) from Mycobacterium leprae (579 aa), FASTA scores: opt: 2330, E(): 1.1e-131, (60.2% identity in 578 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. P96290|FADD28|Rv2941|MTCY24G1.08c (580 aa), FASTA scores: opt: 2587, E(): 4.9e-147, (66.5% identity in 576 aa overlap); O53903|FADD24|Rv1529|MTV045.03 (584 aa), FASTA scores: opt: 2457, E(): 2.9e-139, (63.35% identity in 584 aa overlap); Q50586|FADD25|Rv1521|MT1572|MTCY19G5.07 (583 aa) FASTA scores: opt: 2389, E(): 3.3e-135, (61.45% identity in 581 aa overlap); etc. P9WQ47 No lipid metabolism O07797 P9WQ47 GO:0008152,GO:0016874 6.2.1.- P9WQ47 NC_000962.3 Mycobrowser_v5 CDS 4306867 4307658 . + 0 Rv3833 Rv3833 Involved in a transcriptional mechanism. Transcriptional regulatory protein (probably AraC-family) Rv3833, (MTCY01A6.36c), len: 263 aa. Probable transcriptional regulator belonging to araC family, similar to others e.g. Q9KYN4|SC9H11.05 putative AraC-family transcriptional regulator from Streptomyces coelicolor (289 aa), FASTA scores: opt: 754, E(): 1.2e-42, (50.45% identity in 232 aa overlap); Q9HXH2|PA3830 probable transcriptional regulator from Pseudomonas aeruginosa (270 aa), FASTA scores: opt: 501, E(): 6.2e-26, (34.85% identity in 238 aa overlap); Q9HX87|PA3927 probable transcriptional regulator from Pseudomonas aeruginosa (262 aa), FASTA scores: opt: 496, E(): 1.3e-25, (36.45% identity in 266 aa overlap); P76241|YEAM_ECOLI|B1790 hypothetical transcriptional regulator from Escherichia coli strain K12 (273 aa) FASTA scores: opt: 388, E(): 1.9e-18, (30.5% identity in 223 aa overlap); etc. Contains probable helix-turn-helix motif from aa 164-185, Score 2014 (+6.05 SD). Seems to belong to the AraC/XylS family of transcriptional regulators. P96245 No regulatory proteins P96245 P96245 GO:0006355,GO:0043565,GO:0006350,GO:0003700,GO:0005622 P96245 NC_000962.3 Mycobrowser_v5 CDS 4325495 4325968 . + 0 Rv3853 rraA Binds to RNASE E and inhibits RNASE E endonucleolytic cleavages Regulator of RNase E activity a RraA Rv3853, (MTCY01A6.15c), len: 157 aa. RraA, regulator of RNase E activity A, equivalent to Q9CDD2|RRAA|ML0066 rraA, regulator of RNase E activity a from Mycobacterium leprae (157 aa) FASTA scores: opt: 896, E(): 1.3e-49, (87.1% identity in 155 aa overlap). Also similar to others e.g. P32165|RRAA_ECOLI|B3929|Z5476|ECS4856 from Escherichia coli strain K12 (161 aa), FASTA scores: opt: 428, E(): 3.7e-20, (45.65% identity in 149 aa overlap); etc. Previously known as menG. P9WGY3 No regulatory proteins 1NXJ P0A666 P9WGY3 GO:0008428,GO:0051252 P9WGY3 NC_000962.3 Mycobrowser_v5 CDS 4303398 4305008 . - 0 Rv3829c Rv3829c Function unknown; probably involved in cellular metabolism. Probable dehydrogenase Rv3829c, (MTCY409.01, MTCY01A6.40), len: 536 aa. Probable oxidoreductase dehydrogenase, similar to others e.g. Q9A3T1|CC3121 phytoene dehydrogenase-related protein from Caulobacter crescentus (543 aa), FASTA scores: opt: 607, E(): 9.2e-28, (28.25% identity in 552 aa overlap); Q98FP6|MLR3676 phytoene dehydrogenase from Rhizobium loti (Mesorhizobium loti) (521 aa), FASTA scores: opt: 605, E(): 1.2e-27, (28.2% identity in 546 aa overlap); Q97W24|SSO2422 phytoene dehydrogenase related protein from Sulfolobus solfataricus (518 aa), FASTA scores: opt: 388, E(): 4.4e-15, (27.35% identity in 530 aa overlap); Q98BS8|MLL5443 probable dehydrogenase from Rhizobium loti (Mesorhizobium loti) (524 aa), FASTA scores: opt: 374, E(): 2.9e-14, (24.35% identity in aa overlap); etc. Also similar to MTCY493.22c|Rv1432|MTCY493.22c hypothetical 50.5 KDA protein (probable dehydrogenase) from Mycobacterium tuberculosis (25.1% identity in 295 aa overlap). O07794 No intermediary metabolism and respiration O07794 O07794 GO:0055114,GO:0016491,GO:0009055 1.-.-.- O07794 NC_000962.3 Mycobrowser_v5 CDS 4305056 4305685 . - 0 Rv3830c Rv3830c Involved in transcriptional mechanism. Transcriptional regulatory protein (probably TetR-family) Rv3830c, (MTCY01A6.39), len: 209 aa. Probable transcriptional regulator TetR family, similar to others e.g. P39885|TCMR_STRGA tetracenomycin C transcriptional repressor from Streptomyces glaucescens (226 aa) FASTA scores: opt: 255, E(): 6.1e-10, (33.65% identity in 202 aa overlap); Q9RDR0|SC4A7.02 putative transcriptional regulator from Streptomyces coelicolor (227 aa) FASTA scores: opt: 230, E(): 2.8e-08, (30.05% identity in 213 aa overlap); Q9EWU3|3SC5B7.06 putative regulatory protein from Streptomyces coelicolor (244 aa), FASTA scores: opt: 221, E(): 1.2e-07, (32.05% identity in 181 aa overlap); Q9AJ68|BUTR putative transcriptional repressor from Streptomyces cinnamonensis (268 aa), FASTA scores: opt: 216, E(): 2.7e-07, (37.8% identity in 119 aa overlap); etc. Contains possible helix-turn-helix motif from aa 33-54, Score 1699 (+4.97 SD). Seems to belong to the TetR/AcrR family of transcriptional regulators. P96248 No regulatory proteins P96248 P96248 GO:0006350,GO:0003700,GO:0006355 P96248 NC_000962.3 Mycobrowser_v5 CDS 4310401 4310814 . + 0 Rv3836 Rv3836 Function unknown Conserved hypothetical protein Rv3836, (MTCY01A6.33c), len: 137 aa. Conserved hypothetical protein, highly similar to Q9RKJ2|SCD25.30 hypothetical 13.1 KDA protein from Streptomyces coelicolor (116 aa), FASTA scores: opt: 395, E(): 3.3e-19, (54.4% identity in 114 aa overlap); and similar to CAC47753|SMC0379 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (144 aa) FASTA scores: opt: 194, E(): 6e-06, (33.05% identity in 109 aa overlap); and Q98E37|MLL4425 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (201 aa), FASTA scores: opt: 184, E(): 3.7e-05, (29.75% identity in 121 aa overlap). Contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. P96242 No conserved hypotheticals P96242 P96242 P96242 NC_000962.3 Mycobrowser_v5 CDS 4311704 4312669 . - 0 Rv3838c pheA Involved in L-phenylalanine biosynthesis [catalytic activity: prephenate = phenylpyruvate + H(2)O + CO(2)]. Prephenate dehydratase PheA Rv3838c, (MTCY01A6.31), len: 321 aa. PheA, prephenate dehydratase (see citation below), equivalent to Q9CDC4|PHEA|ML0078 putative prephenate dehydratase from Mycobacterium leprae (322 aa), FASTA scores: opt: 1690, E(): 1.3e-93, (84.25% identity in 311 aa overlap). Also highly similar to others e.g. P10341|PHEA_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (315 aa), FASTA scores: opt: 843, E(): 4e-43, (45.8% identity in 308 aa overlap); Q9ZBX0|SCD78.29c from Streptomyces coelicolor (310 aa), FASTA scores: opt: 820, E(): 9.2e-42, (46.45% identity in 312 aa overlap); Q44104|PHEA_AMYME|PDT from Amycolatopsis methanolica (304 aa), FASTA scores: opt: 707, E(): 4.9e-35, (45.7% identity in 313 aa overlap); etc. Contains PS00858 Prephenate dehydratase signature 2. P9WIC3 No intermediary metabolism and respiration P96240 P9WIC3 GO:0016597,GO:0004664,GO:0042803,GO:0033585 4.2.1.51 P9WIC3 NC_000962.3 Mycobrowser_v5 CDS 4312765 4313541 . + 0 Rv3839 Rv3839 Function unknown Conserved hypothetical protein Rv3839, (MTCY01A6.30c), len: 258 aa. Conserved hypothetical protein, similar in part to Q9RD78|SCF43.10cfrom hypothetical 25.8 KDA protein Streptomyces coelicolor (241 aa), FASTA scores: opt: 270, E(): 3.2e-10, (33.45% identity in 272 aa overlap); and O00320|F25451_2 hypothetical protein from Homo sapiens (Human) (339 aa), FASTA scores: opt: 126, E(): 0.77, (28.75% identity in 240 aa overlap). P96239 No conserved hypotheticals P96239 P96239 NC_000962.3 Mycobrowser_v5 CDS 4342770 4343321 . + 0 Rv3867 espH Function unknown ESX-1 secretion-associated protein EspH Rv3867, (MTV027.02), len: 183 aa. EspH, ESX-1 secretion-associated protein, highly similar to the hypothetical proteins from Mycobacterium leprae: Q9CDD6|ML0056 (169 aa) FASTA scores: opt: 403, E(): 1.8e-18, (48.2% identity in 166 aa overlap); Q49730|ML0407|B1620_C3_264|MLCL383.03 (216 aa), FASTA scores: opt: 517, E(): 1.7e-25, (51.45% identity in 175 aa overlap); and O33090|MLCB628.19c (338 aa), FASTA scores: opt: 403, E(): 3.4e-18, (48.2% identity in 166 aa overlap). Also highly similar to O06269|Rv3614c|MTCY07H7B.08 hypothetical 19.8 KDA protein from Mycobacterium tuberculosis (184 aa), FASTA scores: opt: 559, E(): 3.4e-28, (54.35% identity in 173 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O69732 No cell wall and cell processes O69732 NC_000962.3 Mycobrowser_v5 CDS 4314178 4314723 . + 0 Rv3841 bfrB Involved in iron storage; ferritin is an intracellular molecule that stores iron in a soluble, nontoxic, readily available form. The functional molecule, which is composed of 24 chains, is roughly spherical and contains a central cavity in which the polymeric ferric iron core is deposited. Bacterioferritin BfrB Rv3841, (MTCY01A6.28c), len: 181 aa. bfrB, bacterioferritin, similar to other ferritin or hypothetical proteins e.g. O26261|MTH158|RSGA ferritin like protein from Methanothermobacter thermautotrophicus (171 aa), FASTA scores: opt: 277, E(): 6.6e-11, (30.1% identity in 166 aa overlap); Q99SZ3|SA1709 hypothetical protein from Staphylococcus aureus subsp. aureus N315 (166 aa), FASTA scores: opt: 275, E(): 8.7e-11, (33.35% identity in 156 aa overlap); Q9X0L2|TM1128 ferritin from Thermotoga maritima (164 aa), FASTA scores: opt: 247, E(): 5.3e-09, (25.65% identity in 156 aa overlap); Q9KDT7|BH1124 ferritin from Bacillus halodurans (169 aa), FASTA scores: opt: 246, E(): 6.3e-09, (28.95% identity in 152 aa overlap); O29424|AF0834 putative ferritin from Archaeoglobus fulgidu (169 aa), FASTA scores: opt: 246, E(): 6.3e-09, (28.95% identity in 152 aa overlap); etc. Also shows similarity with Rv1876|MTCY180.42|BFRA probable bacterioferritin from Mycobacterium tuberculosis (159 aa). Seems belong to the bacterioferritin family. P9WNE5 No intermediary metabolism and respiration 3QD8 P96237 P9WNE5 GO:0005737,GO:0008199,GO:0006826,GO:0055114,GO:0016491,GO:0006879 P9WNE5 NC_000962.3 Mycobrowser_v5 CDS 4314738 4315562 . - 0 Rv3842c glpQ1 Glycerophosphoryl diester phosphodiesterase hydrolyzes deacylated phospholipids to G3P and the corresponding alcohols [catalytic activity: a glycerophosphodiester + H(2)O = an alcohol + SN-glycerol 3-phosphate]. Probable glycerophosphoryl diester phosphodiesterase GlpQ1 (glycerophosphodiester phosphodiesterase) Rv3842c, (MTCY01A6.27), len: 274 aa. Probable glpQ1, glycerophosphoryl diester phosphodiesterase, equivalent to Q9CDC5|GLPQ|ML0074 putative glycerophosphoryl diester phosphodiesterase from Mycobacterium leprae (271 aa), FASTA scores: opt: 1635, E(): 1.9e-100, (88.85% identity in 269 aa overlap). Also highly similar to others e.g. CAC44700|SCBAC25E3.13c putative phosphodiesterase from Streptomyces coelicolor (275 aa), FASTA scores: opt: 413, E(): 5.7e-20, (48.05% identity in 258 aa overlap); P37965|GLPQ_BACSU glycerophosphoryl diester phosphodiesterase from Bacillus subtilis (293 aa), FASTA scores: opt: 405, E(): 2e-19, (31.3% identity in 249 aa overlap); Q99VC9|GLPQ|SA0820 glycerophosphoryl diester phosphodiesterase from Staphylococcus aureus subsp. aureus N315 (309 aa) FASTA scores: opt: 341, E(): 3.5e-15, (29.3% identity in 273 aa overlap); etc. P9WMU3 No intermediary metabolism and respiration P96236 P9WMU3 GO:0008889,GO:0006629,GO:0046872,GO:0006071 3.1.4.46 P9WMU3 NC_000962.3 Mycobrowser_v5 CDS 4315568 4316596 . - 0 Rv3843c Rv3843c Unknown Probable conserved transmembrane protein Rv3843c, (MTCY01A6.26), len: 342 aa. Probable conserved transmembrane protein, equivalent to Q9CDC6|ML0073 putative membrane protein from Mycobacterium leprae (344 aa), FASTA scores: opt: 1420, E(): 2.6e-68, (63.05% identity in 349 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P96235 No cell wall and cell processes P96235 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 4320704 4321327 . + 0 Rv3846 sodA Destroys radicals which are normally produced within the cells and are toxic to biological systems [catalytic activity: 2 peroxide radical + 2 H(+) = O(2) + H(2)O(2)]. Superoxide dismutase [FE] SodA Rv3846, (MTCY01A6.22c), len: 207 aa. SodA (alternate gene names: sodB, sod), superoxide dismutase (see citations below), equivalent to many e.g. P47201|SODM_MYCAV|soda|sod from Mycobacterium avium (206 aa), FASTA scores: opt: 1210, E(): 1.8e-73, (82.5% identity in 206 aa overlap); Q9F9R1|sod from Mycobacterium paratuberculosis (207 aa), FASTA scores: opt: 1207, E(): 2.9e-73, (81.65% identity in 207 aa overlap); O86165|SODM_MYCLP|soda|sod from Mycobacterium lepraemurium (206 aa), FASTA scores: opt: 1204, E(): 4.5e-73, (82.05% identity in 206 aa overlap); P13367|SODM_MYCLE|soda|ML0072 from Mycobacterium leprae (206 aa), FASTA scores: opt: 1169, E(): 9.6e-71, (80.5% identity in 205 aa overlap); etc. Contains PS00088 Manganese and iron superoxide dismutases signature. Belongs to the iron/manganese superoxide dismutase family. Although found extracellularly, no signal sequence is present. An alternative secretory pathway may be used. P9WGE7 No virulence, detoxification, adaptation 1GN2,1GN3,1GN4,1GN6,1IDS P17670 P9WGE7 GO:0005576,GO:0046872,GO:0055114,GO:0004784,GO:0006801 1.15.1.1 P9WGE7 NC_000962.3 Mycobrowser_v5 CDS 4324015 4324671 . + 0 Rv3850 Rv3850 Function unknown Conserved protein Rv3850, (MTCY01A6.18c), len: 218 aa. Conserved protein, equivalent to Q9CDD0|ML0068 hypothetical protein from Mycobacterium leprae (238 aa) FASTA scores: opt: 1071, E(): 7.2e-55, (78.35% identity in 217 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P96227 No conserved hypotheticals P96227 NC_000962.3 Mycobrowser_v5 CDS 4327549 4328199 . + 0 Rv3855 ethR Regulates negatively the production of ETHA. Induced ETH resistance when overexpressed in Mycobacterium tuberculosis. Transcriptional regulatory repressor protein (TetR-family) EthR Rv3855, (MTCY01A6.13c), len: 216 aa. EthR (alternate gene names: aka, etaR), regulatory protein TetR family, involved in ethionamide sensitivity/resistance, negatively controls neighbouring ethA (Rv3854c, MTCY01A6.14; alternate gene names: aka etaA) (see citations below). Equivalent to Q9CDD3|ML0064 putative transcriptional regulator from Mycobacterium leprae (214 aa), FASTA scores: opt: 1017, E(): 7e-62, (77.0% identity in 213 aa overlap). Also similar to other transcriptional regulator e.g. Q9S1R1|SCJ9A.09 putative TetR-family transcriptional regulator from Streptomyces coelicolor (204 aa), FASTA scores: opt: 305, E(): 1.2e-13, (34.5% identity in 200 aa overlap); Q9KYT9|SCE22.24 putative TetR-family transcriptional regulator (fragment) from Streptomyces coelicolor (244 aa), FASTA scores: opt: 179, E(): 4.9e-05, (35.5% identity in 93 aa overlap); Q9RUK2|DR1384 transcriptional regulator (TetR family) from Deinococcus radiodurans (196 aa), FASTA scores: opt: 167, E(): 0.00026, (41.75% identity in 79 aa overlap); etc. Also similar to P95100|Rv3058c|MTCY22D7.23 hypothetical 23.8 KDA protein from Mycobacterium tuberculosis (216 aa) FASTA scores: opt: 261, E(): 1.2e-10, (31.65% identity in 221 aa overlap); and O08377|Rv1534|MTCY07A7A.03 hypothetical 24.5 KDA protein from Mycobacterium tuberculosis (225 aa), FASTA scores: opt: 164, E(): 0.00047, (25.5% identity in 248 aa overlap). Contains helix-turn-helix motif at aa 45-66, Score 1320 (+3.68 SD). Belongs to the TetR/AcrR family of transcriptional regulators. P9WMC1 No regulatory proteins 1T56,1U9N,1U9O,3G1L,3G1M,3G1O,3O8G,3O8H,3Q0U,3Q0V,3Q0W,3Q3S,3QPL,3SDG,3TP0,3TP3 P96222 P9WMC1 GO:0006355,GO:0016566,GO:0006350,GO:0003700,GO:0016481 P9WMC1 NC_000962.3 Mycobrowser_v5 CDS 4328401 4329408 . - 0 Rv3856c Rv3856c Function unknown Conserved hypothetical protein Rv3856c, (MTCY01A6.12), len: 335 aa. Conserved hypothetical protein, highly similar to various proteins from diverse organisms e.g. Q9EWR3|3SCF60.21 conserved hypothetical protein from Streptomyces coelicolor (372 aa) FASTA scores: opt: 1286, E(): 2.4e-73, (64.0% identity in 336 aa overlap); P72464|ORF1 from Streptomyces lividans (343 aa), FASTA scores: opt: 1275, E(): 1.1e-72, (60.1% identity in 336 aa overlap); Q9K899|BH3107 DNA-dependent DNA polymerase beta chain from Bacillus halodurans (571 aa), FASTA scores: opt: 592, E(): 1.2e-29, (39.15% identity in 240 aa overlap); etc. May be a DNA polymerase beta (gene name: yshC) (see citation below). P96221 No conserved hypotheticals P96221 P96221 GO:0006260,GO:0003887,GO:0003677 P96221 NC_000962.3 Mycobrowser_v5 CDS 4346481 4348724 . + 0 Rv3870 eccCa1 Unknown ESX conserved component EccCa1. ESX-1 type VII secretion system protein. Possible transmembrane protein. Rv3870, (MTV027.05), len: 747 aa. EccCa1, esx conserved component, ESX-1 type VII secretion system protein, possible transmembrane protein, equivalent to O33087|ML0053|MLCB628.16c putative membrane protein from Mycobacterium leprae (744 aa), FASTA scores: opt: 4333, E(): 0, (85.4% identity in 746 aa overlap); and similar to N-terminal end of others e.g. Q9CD30|ML2535 hypothetical protein from Mycobacterium leprae (1329 aa), FASTA scores: opt: 1003, E(): 1e-52, (33.65% identity in 725 aa overlap); O86653|SC3C3.20c ATP/GTP binding protein from Streptomyces coelicolor (1321 aa), FASTA scores: opt: 1078, E(): 3e-57, (35.4% identity in 774 aa overlap); P71068|YUKA YUKA protein from Bacillus subtilis (1207 aa) FASTA scores: opt: 529, E(): 4.3e-24, (26.1% identity in 636 aa overlap); Q9KE81|BH0975 hypothetical protein from Bacillus halodurans (1489 aa), FASTA scores: opt: 455, E(): 1.5e-19, (27.1% identity in 734 aa overlap); etc. Also similar to N-terminal end of hypothetical proteins from Mycobacterium tuberculosis e.g. O53689|Rv0284|MTV035.12 (1330 aa), FASTA scores: opt: 982, E(): 1.9e-51, (33.8% identity in 719 aa overlap); O06264|Rv3447c|MTCY77.19c (1236 aa), FASTA scores: opt: 761, E(): 4.1e-38, (38.2% identity in 746 aa overlap); O53935|Rv1784|MTV049.06 (932 aa), FASTA scores: opt: 547, E(): 2.8e-25, (36.25% identity in 276 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Note some similarity (with hypothetical proteins from Mycobacterium tuberculosis and P71068|YUKA) continues in downstream ORF MTV027.06. P9WNB3 No cell wall and cell processes O69735 P9WNB3 GO:0003677,GO:0007049,GO:0051301,GO:0007059,GO:0016021,GO:0005886,GO:0005524 P9WNB3 NC_000962.3 Mycobrowser_v5 CDS 4351075 4352181 . + 0 Rv3873 PPE68 Function unknown PPE family protein PPE68 Rv3873, (MTV027.08), len: 368 aa. PPE68, Member of the Mycobacterium tuberculosis PPE family, highly similar to many e.g. O33085|ML0051|MLCB628.14c from Mycobacterium leprae (302 aa), FASTA scores: opt: 656, E(): 2.8e-24, (46.2% identity in 288 aa overlap); and O53691|Rv0286|MTV035.14 (513 aa), FASTA scores: opt: 566, E(): 7.8e-20, (35.25% identity in 363 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted possible vaccine candidate (See Zvi et al., 2008). P9WHW9 No PE/PPE Q79F92 P9WHW9 GO:0005576,GO:0005886,GO:0005618 P9WHW9 NC_000962.3 Mycobrowser_v5 CDS 4383653 4383985 . - 0 Rv3898c Rv3898c Function unknown Conserved hypothetical protein Rv3898c, (MTCY15F10.14), len: 110 aa. Conserved hypothetical protein. Highly similar, but in part, to Q10691|YK83_MYCTU|Rv2083|MT2145|MTCY49.22 hypothetical 30.8 KDA protein from Mycobacterium tuberculosis (314 aa) FASTA scores: opt: 204, E(): 0.00042, (50.6% identity in 81 aa overlap). Similarity suggests it should be in frame with next ORF and that the stop codon could be read through, the sequence appears to be correct. Homology lost upstream at 15138 gatc sequence may suggest discontinuity due to chimerism in cY15F10 or cY49. O05447 No conserved hypotheticals O05447 NC_000962.3 Mycobrowser_v5 CDS 4337946 4338272 . + 0 Rv3861 Rv3861 Unknown Hypothetical protein Rv3861, (MTCY01A6.07c), len: 108 aa. Hypothetical unknown protein. Overlaps in part next ORF Rv3862c|whiB6. P96216 No conserved hypotheticals P96216 NC_000962.3 Mycobrowser_v5 CDS 4331498 4336081 . - 0 Rv3859c gltB Probably involved in glutamate biosynthesis [catalytic activity: 2 L-glutamate + NADP(+) = L-glutamine + 2-oxoglutarate + NADPH]. Probable ferredoxin-dependent glutamate synthase [NADPH] (large subunit) GltB (L-glutamate synthase) (L-glutamate synthetase) (NADH-glutamate synthase) (glutamate synthase (NADH))(NADPH-GOGAT) Rv3859c, (MTCY01A6.09), len: 1527 aa. Probable gltB, ferredoxin-dependent glutamate synthase large subunit, equivalent to Q9CDD5|GLTB|ML0061 putative ferredoxin-dependent glutamate synthase from Mycobacterium leprae (1527 aa), FASTA scores: opt: 9277, E(): 0, (90.25% identity in 1527 aa overlap). Also highly similar to many e.g. Q9S2Y9|SC3A3.04c from Streptomyces coelicolor (1514 aa), FASTA scores: opt: 5939, E(): 0, (64.3% identity in 1544 aa overlap); Q9Z465|GLTB from Corynebacterium glutamicum (Brevibacterium flavum) (1510 aa), FASTA scores: opt: 5790, E(): 0, (63.25% identity in 1534 aa overlap); P39812|GLTB_BACSU|GLTA from Bacillus subtilis (1520 aa), FASTA scores: opt: 3445, E(): 2.8e-196, (52.25% identity in 1531 aa overlap); etc. Similar to other glutamate synthases. P96218 No intermediary metabolism and respiration P96218 P96218 GO:0004355,GO:0055114,GO:0006537 1.4.1.13 P96218 NC_000962.3 Mycobrowser_v5 CDS 4338171 4338521 . - 0 Rv3862c whiB6 Involved in transcriptional mechanism. Possible transcriptional regulatory protein WhiB-like WhiB6 Rv3862c, (MTCY01A6.06), len: 116 aa. Possible whiB6 (alternate gene name: whmF), WhiB-like regulatory protein (see citation below), similar to WhiB paralogue of Streptomyces coelicolor, wblE gene product (85 aa). Shows similarity with Q49765|WHIB7|ML0639|B1937_F2_68 putative transcriptional regulator WHIB7 from Mycobacterium leprae (89 aa) FASTA scores: opt: 112, E(): 0.49, (41.2% identity in 51 aa overlap). Some similarity to Q9AD55|SCP1.95 putative regulatory protein from Streptomyces coelicolor (102 aa) FASTA scores: opt: 129, E(): 0.038, (32.95% identity in 85 aa overlap); AAK47632|MT3290.1 conserved hypothetical protein from Mycobacterium tuberculosis strain CDC1551 (96 aa), FASTA scores: opt: 126, E(): 0.058, (33.35% identity in 84 aa overlap); Q9FC80|SC4B10.07 conserved hypothetical protein from Streptomyces coelicolor (88 aa), FASTA scores: opt: 119, E(): 0.16, (44.65% identity in 70 aa overlap); Q9K4K8|SC5F8.16c regulatory protein from Streptomyces coelicolor (83 aa), FASTA scores: opt: 114, E(): 0.34, (37.05% identity in 54 aa overlap); etc. P9WF37 No regulatory proteins P96215 P9WF37 NC_000962.3 Mycobrowser_v5 CDS 4338849 4340027 . + 0 Rv3863 Rv3863 Unknown Unknown alanine rich protein Rv3863, (MTCY01A6.05c), len: 392 aa. Unknown ala-rich protein. P96214 No conserved hypotheticals P96214 P96214 NC_000962.3 Mycobrowser_v5 CDS 4363417 4364757 . - 0 Rv3883c mycP1 Has proteolytic activity. Cleaves ESPB|Rv3881c. Expressed during infection of macrophages. Membrane-anchored mycosin MycP1 (serine protease) (subtilisin-like protease) (subtilase-like) (mycosin-1) Rv3883c, (MTCY15F10.29), len: 446 aa. MycP1, membrane-anchored serine protease (mycosin) (see citations below), equivalent to O33076|ML0041|MLCB628.04 probable secreted protease from Mycobacterium leprae (446 aa), FASTA scores: opt: 2448, E(): 1.5e-124, (79.15% identity in 446 aa overlap); and highly similar, but in part, to several putative proteases from Mycobacterium leprae; Q9CBV3|ML1538 (567 aa) FASTA scores: opt: 902, E(): 3e-41, (37.25% identity in 556 aa overlap); and Q9CD36|ML2528 (475 aa), FASTA scores: opt: 873, E(): 9.4e-40, (42.7% identity in 459 aa overlap). Shows also similarity with several proteases from other organisms e.g. Q9PCD0|XF1851 serine protease from Xylella fastidiosa (1000 aa), FASTA scores: opt: 281, E(): 1.3e-07, (27.95% identity in 422 aa overlap); P42780|BPRX_BACNO extracellular subtilisin-like protease precursor from Bacteroides nodosus (Dichelobacter nodosus) (595 aa), FASTA scores: opt: 270, E(): 3.2e-07, (28.9% identity in 384 aa overlap); Q46541|APRV5 acidic protease V5 from Bacteroides nodosus (Dichelobacter nodosus) (595 aa), FASTA scores: opt: 264, E(): 6.8e-07, (28.65% identity in 384 aa overlap); etc. Also highly similar to various proteins from Mycobacterium tuberculosis e.g. O53695|Rv0291|MTV035.19 probable membrane-anchored mycosin MYCP3 (461 aa), FASTA scores: opt: 1168, E(): 1.2e-55, (44.6% identity in 453 aa overlap); O53945|Rv1796|MTV049.18 probable membrane-anchored mycosin MYCP5 (585 aa), FASTA scores: opt: 928, E(): 1.2e-42, (37.85% identity in 555 aa overlap) (note gap from aa 155-264); and downstream ORF O05458|Rv3886c|MTCY15F10.26 probable membrane-anchored mycosin MYCP2 (550 aa), FASTA scores: opt: 910, E(): 1.1e-41, (40.15% identity in 533 aa overlap) (note partial gap from aa 146-234); etc. Equivalent to AAK48366 from Mycobacterium tuberculosis strain CDC1551 (411 aa) but longer 35 aa. Has signal sequence with possible signal peptidase I cleavage site in residues 19-21 (ASA) and hydrophobic stretch at C-terminus, followed by short positively charged segment, that seems to act as a membrane anchor. Activated by Ca2+ (see Dave et al., 2002). Contains three serine protease, subtilase family active site motifs: a aspartic acid active site motif (PS00136); a histidine active site motif (PS00137); and a serine active site motif (PS00138). Belongs to peptidase family S8 (also known as the subtilase family), pyrolysin subfamily. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). O05461 No intermediary metabolism and respiration O05461 O05461 GO:0004252,GO:0006508 3.4.21.- O05461 NC_000962.3 Mycobrowser_v5 CDS 4390437 4390709 . - 0 Rv3904c esxE Unknown Putative ESAT-6 like protein EsxE (hypothetical alanine rich protein) (ESAT-6 like protein 12) Rv3904c, (MT4023, MTCY15F10.07), len: 90 aa. EsxE, ESAT-6 like protein, hypothetical unknown ala-rich protein. Belongs to the ESAT6 family (see citation below). P9WNH9 No cell wall and cell processes P9WNH9 NC_000962.3 Mycobrowser_v5 CDS 4341566 4341877 . + 0 Rv3865 espF Function unknown ESX-1 secretion-associated protein EspF Rv3865, (MTCY01A6.03c), len: 103 aa. EspF, ESX-1 secretion-associated protein, showing some similarity to O06268|Rv3615c|MTCY07H7B.07 hypothetical 10.8 KDA protein from Mycobacterium tuberculosis (103 aa), FASTA scores: opt: 198, E(): 7.5e-07, (36.25% identity in 102 aa overlap); Q49723|ML0406|B1620_C2_214|MLCL383.02 hypothetical 11.1 KDA protein from Mycobacterium leprae (106 aa), FASTA scores: opt: 154, E(): 0.00071, (31.05% identity in 103 aa overlap). P9WJD1 No cell wall and cell processes P96212 P9WJD1 NC_000962.3 Mycobrowser_v5 CDS 4371681 4372706 . - 0 Rv3888c Rv3888c Unknown Probable conserved membrane protein Rv3888c, (MTCY15F10.24), len: 341 aa. Probable conserved membrane protein, showing similarity with hypothetical proteins from Mycobacterium leprae: O33082|MLCB628.11c (478 aa), FASTA scores: opt: 530, E(): 7.7e-26, (32.45% identity in 336 aa overlap); Q9CDD8|ML0048 (586 aa), FASTA scores: opt: 530, E(): 9.1e-26, (32.45% identity in 336 aa overlap); Q9CCI1|ML0798 (592 aa), FASTA scores: opt: 426, E(): 3e-19, (27.5% identity in 342 aa overlap) (similarity only at C-terminus). Also similar to proteins from Mycobacterium tuberculosis e.g. P96217|Rv3860|MTCY01A6.08c (390 aa), FASTA scores: opt: 603, E(): 1.7e-30, (35.2% identity in 284 aa overlap); O06396|Rv0530|MTCY25D10.09 (405 aa), FASTA scores: opt: 573, E(): 1.3e-28, (32.0% identity in 328 aa overlap); C-terminus of O69740|Rv3876|MTV027.1 (666 aa), FASTA scores: opt: 509, E(): 2.1e-24, (31.0% identity in 303 aa overlap); etc. O05456 No cell wall and cell processes O05456 O05456 O05456 NC_000962.3 Mycobrowser_v5 CDS 4343314 4345035 . + 0 Rv3868 eccA1 Function unknown ESX conserved component EccA1. ESX-1 type VII secretion system protein. Rv3868, (MTV027.03), len: 573 aa. EccA1, esx conserved component, ESX-1 type VII secretion system protein. Member of the CbxX/CfqX family of hypothetical proteins; C-terminal end is highly similar to many e.g. P40118|CBXC_ALCEU|CBXXC|CFXXC CbxX protein (317 aa) FASTA scores: opt: 572, E(): 3e-24, (42.7% identity in 294 aa overlap); CAC48589 probable CBBX protein from Rhizobium meliloti (Sinorhizobium meliloti) plasmid pSymB (311 aa) FASTA scores: opt: 569, E(): 4.3e-24, (40.05% identity in 292 aa overlap); P95648|CBBX_RHOSH CBBX protein from Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides) (309 aa), FASTA scores: opt: 559, E(): 1.5e-23, (41.4% identity in 290 aa overlap); etc. Equivalent to O33089|Y2G8_MYCLE|ML0055|MLCB628.18c hypothetical 62.3 KDA protein from Mycobacterium leprae (573 aa), FASTA scores: opt: 3330, E(): 3.9e-175, (89.2% identity in 573 aa overlap); and similar to Q9CD28|Y282_MYCLE|ML2537 hypothetical 69.1 KDA protein from Mycobacterium leprae (640 aa), FASTA scores: opt: 943, E(): 2.4e-44, (37.5% identity in 571 aa overlap). Also similar to many proteins from Mycobacterium tuberculosis (strains H37Rv and CDC1551) e.g. O53687|Y282_MYCTU|Rv0282|MT0295|MTV035.10 hypothetical 68.1 KDA protein (631 aa), FASTA scores: opt: 936, E(): 5.8e-44, (39.05% identity in 568 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). P9WPH9 No cell wall and cell processes O69733 P9WPH9 GO:0005737,GO:0017111,GO:0005524 P9WPH9 NC_000962.3 Mycobrowser_v5 CDS 4350745 4351044 . + 0 Rv3872 PE35 Function unknown PE family-related protein PE35 Rv3872, (MTV027.07), len: 99 aa. PE35, Some similarity to Mycobacterium tuberculosis conserved PE family proteins (see Brennan & Delogu 2002), e.g. O69713|Rv3746c|MTV025.094c (111 aa), FASTA scores: opt: 306, E(): 5.5e-13, (50.5% identity in 99 aa overlap). Equivalent to AAK48354 from Mycobacterium tuberculosis strain CDC1551 (112 aa) but shorter 14 aa. P9WIG7 No PE/PPE Q79F93 P9WIG7 NC_000962.3 Mycobrowser_v5 CDS 4352274 4352576 . + 0 Rv3874 esxB Unknown. Exported protein cotranscribed with Rv3875|MT3989|MTV027.10. 10 kDa culture filtrate antigen EsxB (LHP) (CFP10) Rv3874, (MT3988, MTV027.09), len: 100 aa. EsxB, 10 KDA culture filtrate antigen (see citations below, especially first), highly similar to O33084|CF10_MYCLE|ML0050|MLCB628.13c 10 KDA culture filtrate antigen CFP10 homolog from Mycobacterium leprae (99 aa), FASTA scores: opt: 237, E(): 2.4e-08, (39.4% identity in 99 aa overlap). Also similar to O05440|ES6D_MYCTU|Rv3905c|MT4024|MTCY15F10.06 putative ESAT-6 like protein 13 from Mycobacterium tuberculosis (103 aa) FASTA scores: opt: 126, E(): 0.18, (23.1% identity in 91 aa overlap); and shows some similarity with other proteins from Mycobacterium tuberculosis. Contains probable coiled-coil from aa 49-93. Belongs to the ESAT6 family. Note that previously known as lhp (alternate gene name: cfp10). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WNK5 No cell wall and cell processes 1WA8,3FAV P9WNK5 GO:0005515,GO:0005576 P9WNK5 NC_000962.3 Mycobrowser_v5 CDS 4352609 4352896 . + 0 Rv3875 esxA Unknown. Elicits high level of inf-gamma from memory effector cells during the first phase of a protective immune response. Exported protein cotranscribed with Rv3874|MT3988|MTV027.09|LHP|CFP10. 6 kDa early secretory antigenic target EsxA (ESAT-6) Rv3875, (MT3989, MTV027.10), len: 95 aa. EsxA, early secretory antigenic target (see citations below), identical to Q57165|O84901|ESAT6 early secretory antigenic target from Mycobacterium bovis (94 aa), FASTA scores: opt: 596, E(): 4.6e-33, (100.0% identity in 94 aa overlap). Also similar to Q50206|ESA6_MYCLE|ESAT6|ESX|L45|ML0049|MLCB628.12c 6 KDA early secretory antigenic target homolog (ESAT-6-like protein) (L-ESAT) from Mycobacterium leprae (95 aa), FASTA scores: opt: 236, E(): 3.3e-09, (36.25% identity in 91 aa overlap); and weak similarity with others proteins ESAT-like from Mycobacterium leprae. Also some similarity with O53266|ES69_MYCTU|Rv3019c|MT3104|MTV012.33c putative secreted ESAT-6 like protein 9 from Mycobacterium tuberculosis (96 aa), FASTA scores: opt: 131, E(): 0.03, (26.15% identity in 88 aa overlap); and other ESAT-like protein. Contains probable coiled-coil from 56 to 92 aa. Belongs to the ESAT6 family. Note that previously known as esat-6. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted possible vaccine candidate (See Zvi et al., 2008). EspD|Rv3614c expression but not secretion is required for EsxA|Rv3875 secretion (See Chen et al., 2012). P9WNK7 No cell wall and cell processes 1WA8,3FAV P9WNK7 GO:0005515,GO:0005576 P9WNK7 NC_000962.3 Mycobrowser_v5 CDS 4353010 4355010 . + 0 Rv3876 espI Function unknown ESX-1 secretion-associated protein EspI. Conserved proline and alanine rich protein. Rv3876, (MTV027.11), len: 666 aa. EspI, ESX-1 secretion-associated protein, conserved pro-, ala-rich protein, similar to several proteins from Mycobacterium leprae e.g. Q9CDD8|ML0048 hypothetical protein (586 aa), FASTA scores: opt: 1682, E(): 2.1e-45, (50.75% identity in 672 aa overlap); O33082|MLCB628.11c hypothetical 52.0 KDA protein (478 aa), FASTA scores: opt: 1588, E(): 1.5e-42, (53.5% identity in 542 aa overlap) (also has a proline rich N-terminus); etc. Also similar to other proteins from Mycobacterium tuberculosis, especially in C-terminus, e.g. O06396|Rv0530|MTCY25D10.09 (405 aa), FASTA scores: opt: 670, E(): 2.5e-14, (34.85% identity in 396 aa overlap) (also has Pro-rich N-terminus); etc. Note that N-terminus is repetitive and highly Proline rich. P9WJC5 No cell wall and cell processes O69740 P9WJC5 P9WJC5 NC_000962.3 Mycobrowser_v5 CDS 4372800 4373630 . - 0 Rv3889c espG2 Unknown ESX-2 secretion-associated protein EspG2 Rv3889c, (MTCY15F10.23), len: 276 aa. EspG2, ESX-2 secretion-associated protein. P9WJC9 No cell wall and cell processes P9WJC9 P9WJC9 NC_000962.3 Mycobrowser_v5 CDS 4355007 4356542 . + 0 Rv3877 eccD1 Unknown ESX conserved component EccD1. ESX-1 type VII secretion system protein. Probable transmembrane protein. Rv3877, (MTV027.12), len: 511 aa. EccD1, esx conserved component, ESX-1 type VII secretion system protein, probable transmembrane protein, equivalent to Q9CDD9|ML0047 putative membrane protein from Mycobacterium leprae (512 aa), FASTA scores: opt: 2496, E(): 2.8e-140, (74.0% identity in 512 aa overlap); and highly similar, but longer 32 aa, to O33081|MLCB628.10c hypothetical 51.4 KDA protein from Mycobacterium leprae (480 aa), FASTA scores: opt: 2346, E(): 2e-131, (74.15% identity in 480 aa overlap). Shows also similarity with other membrane proteins from Mycobacterium leprae e.g. Q9CBV2|ML1539 probable membrane protein (503 aa), FASTA scores: opt: 318, E(): 2e-11, (22.7% identity in 520 aa overlap). Also similar to various proteins from Mycobacterium tuberculosis e.g. O53944|Rv1795|MTV049.17 putative membrane protein (503 aa), FASTA scores: opt: 391, E(): 9.4e-16, (24.45% identity in 523 aa overlap); O86362|Rv0290|MTV035.18 hypothetical 47.9 KDA protein (472 aa), FASTA scores: opt: 332, E(): 2.8e-12, (28.1% identity in 509 aa overlap); O05457|Rv3887c|MTCY15F10.25 hypothetical 53.2 KDA protein (509 aa), FASTA scores: opt: 167, E(): 0.017, (24.0% identity in 517 aa overlap); etc. Equivalent to AAK48359 from Mycobacterium tuberculosis strain CDC1551 (479 aa) but longer 32 aa. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WNQ7 No cell wall and cell processes O69741 P9WNQ7 GO:0005886,GO:0016021 P9WNQ7 NC_000962.3 Mycobrowser_v5 CDS 4357593 4359782 . - 0 Rv3879c espK Unknown ESX-1 secretion-associated protein EspK. Alanine and proline rich protein. Rv3879c, (MTV027.14c), len: 729 aa. EspK, ESX-1 secretion-associated protein, ala- and pro-rich protein (N-terminal end is repetitive and highly Proline-rich). There may be an unknown protein Orf14 encoded in the opposite orientation, within rv3879c (See Ahmad et al., 1999; Daugelat et al., 2003). P9WJC1 No cell wall and cell processes P9WJC1 GO:0005737 P9WJC1 NC_000962.3 Mycobrowser_v5 CDS 4360199 4360546 . - 0 Rv3880c espL Function unknown ESX-1 secretion-associated protein EspL Rv3880c, (MTV027.15c), len: 115 aa. EspL, ESX-1 secretion-associated protein, equivalent to O33080|ML0044|MLCB628.09 hypothetical 12.2 KDA protein from Mycobacterium leprae (113 aa), FASTA scores: opt: 397, E(): 2e-19, (56.35% identity in 110 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WJB9 No cell wall and cell processes O69744 P9WJB9 NC_000962.3 Mycobrowser_v5 CDS 4374049 4374372 . - 0 Rv3891c esxD Function unknown Possible ESAT-6 like protein EsxD Rv3891c, (MTCY15F10.21), len: 107 aa (first GTG taken). EsxD, ESAT-6 like protein, equivalent to Q9K547 hypothetical 10.3 KDA protein (fragment) from Mycobacterium paratuberculosis (100 aa), FASTA scores: opt: 498, E(): 1.7e-26, (77.25% identity in 101 aa overlap). Seems to belong to the ESAT6 family (see Gey Van Pittius et al., 2001). O05453 No cell wall and cell processes O05453 NC_000962.3 Mycobrowser_v5 CDS 4390720 4391031 . - 0 Rv3905c esxF Unknown Putative ESAT-6 like protein EsxF (hypothetical alanine and glycine rich protein) (ESAT-6 like protein 13) Rv3905c, (MT4024, MTCY15F10.06), len: 103 aa. EsxF, ESAT-6 like protein (see citation below), hypothetical unknown ala-, gly-rich protein, ESAT-6 like protein. Belongs to the ESAT6 family. P9WNH7 No cell wall and cell processes P9WNH7 NC_000962.3 Mycobrowser_v5 CDS 4366908 4368521 . - 0 Rv3885c eccE2 Unknown ESX conserved component EccE2. ESX-2 type VII secretion system protein. Possible membrane protein. Rv3885c, (MTCY15F10.27), len: 537 aa. eccE2, esx conserved component, ESX-2 type VII secretion system protein. possible membrane protein (has hydrophobic stretch near N-terminus), showing some similarity with O05462|Rv3882c|MTV027.17c|MTCY15F10.30 possible membrane protein from Mycobacterium tuberculosis (462 aa) FASTA scores: opt: 283, E(): 8.3e-10, (26.55% identity in 414 aa overlap); and O33077|ML0042|MLCB628.05 putative membrane protein from Mycobacterium leprae (467 aa), FASTA scores: opt: 260, E(): 2.1e-08, (28.0% identity in 382 aa overlap). Equivalent to AAK48368 from Mycobacterium tuberculosis strain CDC1551 (422 aa) but longer 115 aa. P9WJE7 No cell wall and cell processes O05459 P9WJE7 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 4370155 4371684 . - 0 Rv3887c eccD2 Unknown ESX conserved component EccD2. ESX-2 type VII secretion system protein. Probable transmembrane protein. Rv3887c, (MTCY15F10.25), len: 509 aa. eccD2, esx conserved component, ESX-2 type VII secretion system protein, probable transmembrane protein (has hydrophilic stretch from ~1-130 then very hydrophobic domain), similar to other membrane proteins and with weak similarity to known transporters, e.g. Q9CBV2|ML1539 probable membrane protein from Mycobacterium leprae (503 aa), FASTA scores: opt: 395, E(): 2.3e-16, (28.0% identity in 496 aa overlap); Q9CD35|ML2529 conserved membrane protein from Mycobacterium leprae (485 aa), FASTA scores: opt: 221, E(): 6.6e-06, (24.6% identity in 423 aa overlap); Q9ADP8|2SC10A7.11 putative integral membrane protein from Streptomyces coelicolor (430 aa), FASTA scores: opt: 171, E(): 0.0062, (26.55% identity in 358 aa overlap); CAC44275|SCBAC17F8.03 putative drug efflux protein from Streptomyces coelicolor (416 aa), FASTA scores: opt: 160, E(): 0.028, (27.85% identity in 323 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. O53944|Rv1795|MTV049.17 putative membrane protein (503 aa), FASTA scores: opt: 360, E(): 2.9e-14, (26.65% identity in 514 aa overlap); etc. Equivalent to AAK48369 from Mycobacterium tuberculosis strain CDC1551 (469 aa) but longer 40 aa. P9WNQ5 No cell wall and cell processes O05457 P9WNQ5 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 4391097 4391606 . - 0 Rv3906c Rv3906c Function unknown Conserved hypothetical protein Rv3906c, (MTCY15F10.05), len: 169 aa. Conserved hypothetical protein, strongly related to Q50578|AT9S (sod related in Escherichia coli) from Mycobacterium tuberculosis strain aoyama B (155 aa), but apparently different as flanking sequences differ and shorter 43 aa, FASTA scores: opt: 548, E(): 1.3e-26, (79.4% identity in 102 aa overlap). Selfmarch results suggest that Rv3906c is not related to any other hypothetical protein from Mycobacterium tuberculosis strain H37Rv except itself. Shows also similarity with Q9VFR2|CG9297 hypothetical protein from Drosophila melanogaster (Fruit fly) (930 aa), FASTA scores: opt: 221, E(): 4.9e-06, (36.95% identity in 157 aa overlap); Q9HQ55|CBP|VNG1320G calcium-binding protein homology from Halobacterium sp. strain NRC-1 (385 aa) FASTA scores: opt: 143, E(): 0.13, (35.65% identity in 160 aa overlap); Q24795 calcium-binding protein (fragment) from Echinococcus granulosus (338 aa), FASTA scores: opt: 140, E(): 0.17, (33.95% identity in 156 aa overlap). O05439 No conserved hypotheticals O05439 O05439 NC_000962.3 Mycobrowser_v5 CDS 4376262 4380452 . - 0 Rv3894c eccC2 Unknown ESX conserved component EccC2. ESX-2 type VII secretion system protein. Possible membrane protein. Rv3894c, (MTCY15F10.18), len: 1396 aa. EccC2, esx conserved component, ESX-2 type VII secretion system protein, possible membrane protein (possible transmembrane segments from aa ~37-85), similar to Q9CD30|ML2535 hypothetical protein from Mycobacterium leprae (1329 aa), FASTA scores: opt: 652, E(): 2.2e-30, (27.85% identity in 1425 aa overlap); Q9CDD7|ML0052 hypothetical protein from Mycobacterium leprae (597 aa), FASTA scores: opt: 537, E(): 6.6e-24, (27.5% identity in 585 aa overlap) (similarity only with C-terminal end); Q9Z5I2|ML1543|MLCB596.28 possible SPOIIIE-family membrane protein from Mycobacterium leprae (1345 aa), FASTA scores: opt: 523, E(): 8.6e-23, (31.65% identity in 1412 aa overlap). Also similar to various proteins e.g. O86653|SC3C3.20c ATP/GTP binding protein from Streptomyces coelicolor (1321 aa), FASTA scores: opt: 973, E(): 2.8e-49, (28.1% identity in 1409 aa); Q9L0T6|SCD35.15c putative cell division-related protein from Streptomyces coelicolor(1525 aa), FASTA scores: opt: 524, E(): 8.3e-23, (24.95% identity in 1450 aa overlap); Q9KE81|BH0975 hypothetical protein from Bacillus halodurans (1489 aa), FASTA scores: opt: 444, E(): 4.1e-18, (22.5% identity in 1346 aa overlap); etc. Also similar to AAK46103|MT1833 FTSK/SPOIIIE family protein from Mycobacterium tuberculosis strain CDC1551 (1391 aa), FASTA scores: opt: 769, E(): 2.9e-37, (30.6% identity in 1434 aa overlap); and other hypothetical proteins from Mycobacterium tuberculosis e.g. O53689|Rv0284|MTV035.12 (1330 aa), FASTA scores: opt: 634, E(): 2.5e-29, (28.2% identity in 1443 aa overlap); O06264|Rv3447c|MTCY77.19c (1236 aa), FASTA scores: opt: 632, E(): 3.1e-29, (28.75% identity in 1391 aa overlap); O69736|R3871|MTV027.06 (591 aa), FASTA scores: opt: 588, E(): 6.6e-27, (27.75% identity in 605 aa overlap) (similarity only with C-terminal end); etc. Contains two possible (PS00017) ATP/GTP-binding sites (P-loop) in central portion. O05450 No cell wall and cell processes O05450 O05450 GO:0003677,GO:0007049,GO:0051301,GO:0007059,GO:0016021,GO:0005524 O05450 NC_000962.3 Mycobrowser_v5 CDS 4381943 4382851 . - 0 Rv3896c Rv3896c Function unknown Conserved hypothetical protein Rv3896c, (MTCY15F10.16), len: 302 aa (first GTG taken, although TBParse suggests TTG at 16079). Putative conserved ala-rich protein. C-terminus highly similar to C-terminal end of other proteins e.g. Q9XAS4|SC10A7.01 hypothetical 17.2 KDA protein from Streptomyces coelicolor (244 aa), FASTA scores: opt: 255, E(): 1.4e-08, (32.0% identity in 222 aa overlap); CAC44611|STBAC16H6.32 putative secreted protein from Streptomyces coelicolor (172 aa), FASTA scores: opt: 214, E(): 3.4e-06, (42.55% identity in 94 aa overlap); Q38352|ORF360 from Lactococcus delbrueckii bacteriophage ll-H (360 aa), FASTA scores: opt: 211, E(): 9.5e-06, (40.0% identity in 115 aa overlap); P54334|XKDO_BACSU|XKDO phage-like element PBSX protein from Bacillus subtilis (1332 aa), FASTA scores: opt: 209, E(): 3.6e-05, (38.35% identity in 86 aa overlap); etc. Also similar to P71594|P71594|Rv0024|MTCY10H4.24 hypothetical 30.3 KDA protein from Mycobacterium tuberculosis (281 aa), FASTA scores: opt: 265, E(): 3.9e-09, (29.25% identity in 287 aa overlap). O05448 No conserved hypotheticals O05448 O05448 NC_000962.3 Mycobrowser_v5 CDS 4387892 4390432 . - 0 Rv3903c Rv3903c Unknown Hypothetical alanine and proline rich protein Rv3903c, (MTCY15F10.08), len: 846 aa. Hypothetical unknown ala-, pro-rich protein. O05442 No conserved hypotheticals O05442 O05442 NC_000962.3 Mycobrowser_v5 CDS 4393449 4394195 . + 0 Rv3908 mutT4 Function unknown Possible mutator protein MutT4 Rv3908, (MTCY15F10.03c), len: 248 aa. Possible mutT4, mutator protein, equivalent to Q50195|ML2698|L222-ORF6 hypothetical protein from Mycobacterium leprae (251 aa), FASTA scores: opt: 1270, E(): 3.4e-62, (79.05% identity in 248 aa overlap). Also similar to O66548|APFA|AQ_158 hydrolase from Aquifex aeolicus (134 aa), FASTA scores: opt: 300, E(): 1.1e-09, (37.3% identity in 142 aa overlap); and similarity with other various proteins e.g. O93721 diadenosine 5'5'''-P1,P4-tetraphosphate pyrophosphohydrolase from Pyrobaculum aerophilum (143 aa), FASTA scores: opt: 205, E(): 0.00017, (34.85% identity in 109 aa overlap); Q9HS29|APA|VNG0431G diadenosine tetraphosphate pyrophosphohydrolase from Halobacterium sp. strain NRC-1 (142 aa), FASTA scores: opt: 199, E(): 0.00036, (34.0% identity in 147 aa overlap); Q9YA58|APE2080 hypothetical 19.2 KDA protein from Aeropyrum pernix (175 aa) FASTA scores: opt: 191, E(): 0.0012, (36.9% identity in 141 aa overlap); etc. Also similar to P95110|MUTT1|Rv2985|MTCY349.02 hypothetical 34.7 KDA protein from Mycobacterium tuberculosis (317 aa) FASTA scores: opt: 224, E(): 3e-05, (34.05% identity in 144 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). Seems to belong to the NUDIX hydrolase family. P9WIX7 No information pathways O05437 P9WIX7 GO:0046872,GO:0016787 3.6.1.- P9WIX7 NC_000962.3 Mycobrowser_v5 CDS 4402732 4403082 . + 0 Rv3914 trxC Thioredoxin participates in various redox reactions through the reversible oxidation of its active center dithiol, to a disulfide, & catalyzes dithiol-disulfide exchange reactions. Forms together with thioredoxin reductase and NADPH a redox active system which donates electrons to a wide variety of different metabolic process. Seems regulated by sigh (Rv3223c product). Thioredoxin TrxC (TRX) (MPT46) Rv3914, (MT4033, MTV028.05), len: 116 aa. TrxC (alternate gene names: mpt46, trx, trxA *), thioredoxin (see citations below), equivalent to O30974|THIO_MYCSM|TRXA thioredoxin from Mycobacterium smegmatis (112 aa), FASTA scores: opt: 576, E(): 2.1e-32, (80.2% identity in 111 aa overlap); and also equivalent to C-terminal end of P46843|TRXB_MYCLE|TRXB/a|TRX|ML2703 bifunctional thioredoxin reductase/thioredoxin from Mycobacterium leprae (458 aa), FASTA scores: opt: 628, E(): E(): 2e-35, (82.9% identity in 117 aa overlap). Also highly similar to many e.g. P80579|THIO_ALIAC from Alicyclobacillus acidocaldarius (Bacillus acidocaldarius) (105 aa), FASTA scores: opt: 411, E(): 3e-21, (57.15% identity in 105 aa overlap); P00275|THI1_CORNE from Corynebacterium nephridii (105 aa), FASTA scores: opt: 394, E(): 4.3e-20, (56.7% identity in 97 aa overlap); P00274|THIO_ECOLI|TRXA|TSNC|FIPA|B3781 from Escherichia coli and Salmonella typhimurium strain K12 and LT2 respectively (108 aa), FASTA scores: opt: 364, E(): 4.7e-18, (54.45% identity in 101 aa overlap); etc. Also similar to O53162|TRXB|Rv1471|MTV007.18 thioredoxin from Mycobacterium tuberculosis (123 aa), FASTA scores: E(): 2.3e-15, (41.9% identity in 93 aa overlap). Contains PS00194 Thioredoxin family active site. Belongs to the thioredoxin family. The product of this CDS is supposedly secreted. In this case, this protein could exert its free radical scavenging activity inside macrophages. (*) Warning: note that Rv1470|MTV007.17 correspond also to trxA. P9WG67 No intermediary metabolism and respiration 2I1U,3O6T,2L4Q,2L59 P0A616 P9WG67 GO:0009055,GO:0022900,GO:0006662,GO:0015035,GO:0006810,GO:0045454 P9WG67 NC_000962.3 Mycobrowser_v5 CDS 4396597 4400151 . + 0 Rv3910 Rv3910 Unknown Probable conserved transmembrane protein Rv3910, (MTCY15F10.01c.MTV028.01), len: 1184 aa. Probable conserved transmembrane protein (hydrophobic domain ~50-550), equivalent to Q9CCX9|ML2700 possible conserved membrane protein from Mycobacterium leprae (1206 aa), FASTA scores: opt: 5554, E(): 0, (75.15% identity in 1182 aa overlap); and highly similar, but shorter 380 aa, to Q50199|L222-ORF10 from Mycobacterium leprae (784 aa) FASTA scores: opt: 3297, E(): 5.5e-170, (68.8% identity in 769 aa overlap); and at the N-terminal end with Q50198|L222-ORF also from Mycobacterium leprae (379 aa) FASTA scores: opt: 1955, E(): 5.7e-98, (88.4% identity in 353 aa overlap) (ORFs 9 and 10 are adjacent on L222). Also similar in part (principally at the N-terminal end) to other membrane proteins e.g. Q9X8T0|SCH24.16c putative transmembrane protein from Streptomyces coelicolor (811 aa), FASTA scores: opt: 573, E(): 2.8e-23, (31.05% identity in 573 aa overlap); O05467|MVIN_RHITR integral membrane protein virulence factor MVIN homolog from Rhizobium tropici (533 aa), FASTA scores: opt: 468, E(): 9e-18, (27.1% identity in 524 aa overlap); P56882|MVIN_RHIME integral membrane protein virulence factor MVIN homolog from Rhizobium meliloti (Sinorhizobium meliloti) (535 aa), FASTA scores: opt: 453, E(): 5.8e-17, (26.2% identity in 557 aa overlap); etc. P9WJK3 No cell wall and cell processes O05435 P9WJK3 GO:0016021 P9WJK3 NC_000962.3 Mycobrowser_v5 CDS 4401728 4402735 . + 0 Rv3913 trxB2 Enzyme that catalyse the reduction of disulphides by pyridine nucleotides through an enzyme disulphide and a flavin. Seems regulated by sigh (Rv3223c product). [catalytic activity: NADPH + oxidized thioredoxin = NADP(+) + reduced thioredoxin]. Probable thioredoxin reductase TrxB2 (TRXR) (TR) Rv3913, (MT4032, MTV028.04), len: 335 aa. Probable trxB2, thioredoxin reductase (see citation below), equivalent to O30973|TRXB_MYCSM thioredoxin reductase from Mycobacterium smegmatis (311 aa), FASTA scores: opt: 1575, E(): 1.8e-87, (78.35% identity in 305 aa overlap); and highly similar, but shorter at C-terminus, to P46843|TRXB_MYCLE|TRXB/a|TRX|ML2703 bifunctional thioredoxin reductase/thioredoxin from Mycobacterium leprae (458 aa), FASTA scores: opt: 1766, E(): 8.7e-99, (83.25% identity in 328 aa overlap). Also highly similar to many e.g. P52215|TRXB_STRCO|SCH24.12 from Streptomyces coelicolor (321 aa), FASTA scores: opt: 1249, E(): 7.2e-68, (60.4% identity in 313 aa overlap); Q9Z8M4|TRXB_CHLPN from Chlamydia pneumoniae (Chlamydophila pneumoniae) (311 aa), FASTA scores: opt: 978, E(): 1.3e-51, (49.85% identity in 307 aa overlap); P09625|TRXB_ECOLI|B0888 from Escherichia coli strain K12 (320 aa), FASTA scores: opt: 948, E(): 8.6e-50, (49.2% identity in 309 aa overlap); etc. Contains PS00573 Pyridine nucleotide-disulphide oxidoreductases class-II active site. Belongs to the pyridine nucleotide-disulfide oxidoreductases class-II. Cofactor: FAD (by similarity). P9WHH1 No intermediary metabolism and respiration 2A87 P52214 P9WHH1 GO:0005737,GO:0055114,GO:0019430,GO:0004791,GO:0050660 1.8.1.9 P9WHH1 NC_000962.3 Mycobrowser_v5 CDS 4406488 4407531 . - 0 Rv3918c parA Involved in chromosome partition. Localize to both POLES of the predivisional cell following completion of DNA replication. Probable chromosome partitioning protein ParA Rv3918c, (MTV028.09c), len: 347 aa. Probable parA, chromosome partitioning protein, highly similar to Q9CCX7|para|ML2707 putative cell division protein from Mycobacterium leprae (351 aa), FASTA scores: opt: 1679, E(): 2.9e-93, (78.1% identity in 347 aa overlap). Also highly similar to others e.g. Q9RFM1|para para protein from Streptomyces coelicolor (357 aa), FASTA scores: opt: 1197, E(): 2e-64, (60.45% identity in 306 aa overlap); Q98DZ3|MLL4479|para chromosome partitioning protein from Rhizobium loti (Mesorhizobium loti) (266 aa), FASTA scores: opt: 835, E(): 7.2e-43, (50.95% identity in 257 aa overlap); O05189|PARA_CAUCR chromosome partitioning protein from Caulobacter crescentus (267 aa), FASTA scores: opt: 813, E(): 1.5e-41, (51.35% identity in 261 aa overlap) (has its N-terminus shorter); etc. Equivalent to AAK48403 from Mycobacterium tuberculosis strain CDC1551 (381 aa) but shorter 34 aa. Also similar to other Mycobacterium tuberculosis proteins: MTCI125.30, FASTA scores: E(): 4.3e-32, (35.2% identity in 327 aa overlap); and MTCY07D11.13, FASTA scores: E(): 3e-30, (39.9% identity in 263 aa overlap). Belongs to the para family. Possible alternative start site at aa 107. Note that previously known as parB. Q1LVD4 No cell wall and cell processes Q1LVD4 Q1LVD4 Q1LVD4 NC_000962.3 Mycobrowser_v5 CDS 4404433 4405167 . - 0 Rv3916c Rv3916c Function unknown Conserved hypothetical protein Rv3916c, (MTV028.07c), len: 244 aa. Conserved hypothetical protein, equivalent to Q50200|ML2705|L222-ORF1 hypothetical protein from Mycobacterium leprae (259 aa), FASTA scores: opt: 1266, E(): 2e-74, (76.4% identity in 250 aa overlap). Also highly similar (but with gaps) to Q9R3S2|STH24.10 hypothetical 22.6 KDA protein from Streptomyces coelicolor (205 aa), FASTA scores: opt: 387, E(): 7.5e-18, (40.25% identity in 231 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). O53594 No conserved hypotheticals O53594 O53594 NC_000962.3 Mycobrowser_v5 CDS 4407528 4408202 . - 0 Rv3919c gid Unknown Probable glucose-inhibited division protein B Gid Rv3919c, (MT4038, MTV028.10c), len: 224 aa. Probable gid (alternate gene name: gidB), glucose-inhibited division protein B, equivalent, but shorter 20 aa, to Q9L7M3 putative GIDB (fragment) from Mycobacterium paratuberculosis (245 aa), FASTA scores: opt: 1018, E(): 4.8e-57, (73.95% identity in 211 aa overlap); and Q50203|GIDB_MYCLE|ML2708 glucose inhibited division protein B from Mycobacterium leprae (245 aa), FASTA scores: opt: 966, E(): 9.1e-54, (68.4% identity in 212 aa overlap). Also highly similar to many e.g. O54571|GIDB_STRCO|STH24.07 from Streptomyces coelicolor (239 aa), FASTA scores: opt: 654, E(): 3.9e-34, (47.95% identity in 221 aa overlap); Q9KNG5|VC2774 from Vibrio cholerae (210 aa), FASTA scores: opt: 300, E(): 6.9e-12, (38.15% identity in 139 aa overlap); P17113|GIDB_ECOLI|B3740|Z5240|ECS4682 from Escherichia coli (several strains) (207 aa), FASTA scores: opt: 287, E(): 4.5e-11, (34.8% identity in 138 aa overlap); etc. Contains PS00539 Pyrokinins signature. Belongs to the GIDB family. Nucleotide position 4407904 in the genome sequence has been corrected, G:A resulting in S100F. P9WGW9 No cell wall and cell processes O53597 P9WGW9 GO:0070043,GO:0006364,GO:0005737 2.1.1.- SM P9WGW9 NC_000962.3 Mycobrowser_v5 CDS 4408334 4408897 . - 0 Rv3920c Rv3920c Unknown Conserved protein similar to jag protein Rv3920c, (MTV028.11c), len: 187 aa. Conserved protein, similar to jag protein, equivalent to Q9L7M2 hypothetical 20.1 KDA protein from Mycobacterium paratuberculosis (183 aa), FASTA scores: opt: 1004, E(): 7.3e-52, (85.05% identity in 187 aa overlap); and Q50204|ML2709 hypothetical protein similar to jag protein SPOIIIJ associated protein in bacillus subtilis from Mycobacterium leprae (193 aa), FASTA scores: opt: 871, E(): 4.4e-44, (73.05% identity in 193 aa overlap). Also similar to other bacterial proteins e.g. O54595|STH24.06|jag jag-like protein from Streptomyces coelicolor (170 aa), FASTA scores: opt: 593, E(): 6.7e-28, (62.85% identity in 167 aa overlap); Q9RCA6|jag|BH4063 jag protein homolog from Bacillus halodurans (207 aa), FASTA scores: opt: 282, E(): 1.1e-09, (35.0% identity in 140 aa overlap); Q9X1H1|TM1460 putative jag protein, putative from Thermotoga maritima (221 aa), FASTA scores: opt: 258, E(): 3e-08, (31.9% identity in 138 aa overlap);Q01620|JAG_BACSU jag protein (SPOIIIJ associated protein) from Bacillus subtilis (208 aa), FASTA scores: opt: 196, E(): 0.00012, (28.05% identity in 139 aa overlap); etc. O53598 No conserved hypotheticals O53598 O53598 GO:0003676 O53598 NC_000962.3 Mycobrowser_v5 CDS 3593369 3593852 . + 0 Rv3216 Rv3216 Acetylation, substrate unknown GCN5-related N-acetyltransferase, pseudogene Rv3216, (MTCY07D11.10c), len: 160 aa. Acetyltransferase (2.3.1.-), contains GNAT domain (Gcn5-related N-acetyltransferase. See Vetting et al. 2005), probably pseudogene as appears frameshifted due to 1bp insertion at position 3593438. Frameshift present in all sequenced tubercle bacilli. Start changed since first submission, extended by 50aa. Similar to many acetyltransferases e.g. Q9AB32|CC0402 acetyltransferase (GNAT family) from Caulobacter crescentus (159 aa), FASTA scores: opt: 325, E(): 3.8e-17, (45.65% identity in 103 aa overlap); P79081|ATS1 putative acetyltransferase ATS1 from Schizosaccharomyces pombe (Fission yeast) (168 aa), FASTA scores: opt: 313, E(): 3.1e-16, (47.6% identity in 105 aa overlap). O05850 Yes intermediary metabolism and respiration O05850 O05850 GO:0008152,GO:0008080 2.3.1.- NC_000962.3 Mycobrowser_v5 CDS 4410786 4410929 . - 0 Rv3924c rpmH Involved in translation mechanism. This protein is one of the early assembly proteins of the 50S ribosomal subunit. 50S ribosomal protein L34 RpmH Rv3924c, (MTV028.15), len: 47 aa. rpmH, 50s ribosomal protein l34 (see citations below), equivalent to many mycobacterial 50S ribosomal protein L34 e.g. P46386|RL34_MYCLE|RPMH|ML2713 from Mycobacterium leprae (47 aa), FASTA scores: opt: 287, E(): 8.5e-17, (91.5% identity in 47 aa overlap); and Q9L7L8|RL34_MYCPA|RPMH from Mycobacterium paratuberculosis (47 aa), FASTA scores: opt: 281, E(): 2.6e-16, (89.35% identity in 47 aa overlap). Also highly similar to other ribosomal proteins e.g. P27901|RL34_STRCO|RPMH|STH24.02 from Streptomyces coelicolor (45 aa), FASTA scores: opt: 234, E(): 1.4e-12, (79.05% identity in 43 aa overlap); and P05647|RL34_BACSU|RPMH from Bacillus subtilis (44 aa) FASTA scores: opt: 229, E(): 3.7e-12, (72.35% identity in 47 aa overlap); etc. Contains PS00784 Ribosomal protein L34 signature. Belongs to the L34P family of ribosomal proteins. P9WH93 No information pathways P0A5W4 P9WH93 GO:0003735,GO:0006412,GO:0005840 P9WH93 NC_000962.3 Mycobrowser_v5 CDS 333160 333417 . - 0 Rv0277A vapB25 Unknown Possible antitoxin VapB25 Rv0277A, len: 85 aa. Possible vapB25, antitoxin, part of toxin-antitoxin (TA) operon with Rv0277c, see Arcus et al. 2005. Has in-frame stop codon so may not be expressed. Very similar to others in Mycobacterium tuberculosis e.g. Rv0748 (85 aa). Fasta score E(): 4e-24; 88.2% identity in 85 aa overlap P0CW33 Yes virulence, detoxification, adaptation P0CW33 P0CW33 NC_000962.3 Mycobrowser_v5 CDS 2725571 2726087 . - 0 Rv2427A oxyR' Transcriptional regulator OxyR', pseudogene Rv2427A, Pseudogene oxyR', inactivated by multiple mutations; identical to sequence in u16243 (see Deretic et al., 1995). Yes virulence, detoxification, adaptation INH NC_000962.3 Mycobrowser_v5 CDS 4410053 4410415 . - 0 Rv3922c Rv3922c Unknown Possible hemolysin Rv3922c, (MTV028.13c), len: 120 aa. Possible hemolysin, highly similar to Q9L7M0|YIDD_MYCPA hypothetical 12.4 KDA protein from Mycobacterium paratuberculosis (115 aa), FASTA scores: opt: 521, E(): 1.9e-29, (65.2% identity in 112 aa overlap). Also highly similar to Q44066|HLYA_AERHY putative alpha-hemolysin from Aeromonas hydrophila (85 aa), FASTA scores: opt: 276, E(): 1.5e-12, (51.45% identity in 70 aa overlap); and to many bacterial hypothetical proteins from bacterium e.g. P22847|YIDD_ECOLI|B3704.1 hypothetical protein from Escherichia coli strain K12 (85 aa), FASTA scores: opt: 276, E(): 1.5e-12, (51.45% identity in 70 aa overlap). P9WFL9 No virulence, detoxification, adaptation P67300 P9WFL9