Refseq_ID Mycobrowser_Version Feature Start Stop Score Strand Frame Locus Name Function Product Comments UniProt_AC Is_Pseudogene Functional_Category Protein Data Bank PFAM UniProt Gene Ontology Enzyme Classification Drug Resistance Mutations InterPro UniProtKB/TrEMBL SWISS-MODEL Orthologues M. leprae Orthologues M. marinum Orthologues M. smegmatis Orthologues M. bovis Orthologues M. lepromatosis Orthologues M. tuberculosis Orthologues M. abscessus Orthologues M. haemophilum Orthologues M. orygis Orthologues M. tuberculosis NC_000962.3 Mycobrowser_v5 ncRNA 759479 759610 . + MTB000115 ncRv10666 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 786021 786074 . + MTB000116 ncRv10685 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 80240 80440 . + MTB000100 ncRv10071 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 80254 80344 . - MTB000101 ncRv10071c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 156452 156567 . + MTB000102 ncRv10128 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 156521 156568 . - MTB000103 ncRv10128c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 177236 177285 . - MTB000104 ncRv10150c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 209683 209841 . + MTB000105 ncRv0179 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 218320 218379 . - MTB000106 ncRv0186c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 293603 293663 . + MTB000107 ncRv10243 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 530246 530353 . - MTB000108 ncRv0441c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 558815 558884 . + MTB000109 ncRv10467 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 579290 579408 . + MTB000110 ncRv0490 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 704185 704246 . + MTB000111 ncRv10609 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 733361 733459 . + MTB000112 ncRv10637 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 734118 734244 . + MTB000113 ncRv0638 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 736166 736284 . + MTB000114 ncRv0641 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 800242 800359 . + MTB000117 ncRv10699 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 815417 815685 . + MTB000118 ncRv0724 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 905075 905164 . - MTB000119 ncRv0810c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 958459 958509 . + MTB000120 ncRv10860 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1000719 1000826 . + MTB000121 ncRv0897 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1063969 1064101 . + MTB000122 ncRv0952 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1113606 1113664 . + MTB000123 ncRv10996 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1165548 1165613 . - MTB000124 ncRv11042c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1197082 1197179 . + MTB000125 ncRv1072 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1271918 1271961 . - MTB000126 ncRv11144c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1275610 1275674 . - MTB000127 ncRv11147c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1313343 1313452 . - MTB000128 ncRv11179c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1342888 1342941 . + MTB000129 ncRv11199 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1821646 1821753 . - MTB000138 ncRv1621c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1960667 1960774 . + MTB000139 ncRv11733 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 2030986 2031038 . + MTB000140 ncRv11793 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 2068863 2068962 . + MTB000141 ncRv1821 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 2096766 2096867 . + MTB000142 ncRv11846 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 2268164 2268231 . + MTB000143 ncRv12023 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 2489205 2489252 . + MTB000144 ncRv12220 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 2762409 2762484 . + MTB000145 ncRv12459 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 2877751 2877808 . + MTB000146 ncRv12557 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 2966410 2966450 . + MTB000147 ncRv12641 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 3092761 3092886 . - MTB000148 ncRv12783c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 3343113 3343216 . - MTB000149 ncRv2986c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 3350950 3351074 . - MTB000150 ncRv2993c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 3363029 3363152 . - MTB000151 ncRv13003c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 3595951 3596059 . + MTB000152 ncRv3220 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 3690941 3691059 . + MTB000153 ncRv13303 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 3837346 3837458 . - MTB000155 ncRv13418cB Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 3880231 3880294 . - MTB000156 ncRv3461c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 3956291 3956550 . + MTB000157 ncRv3520 Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 4088267 4088350 . - MTB000158 ncRv3648c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 4099384 4099477 . - MTB000159 ncRv13660c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 4168192 4168281 . - MTB000160 ncRv13722c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 4265583 4265765 . - MTB000161 ncRv3804c Unknown Putative small regulatory RNA Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017) No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 1446193 1446265 . - 0 MTB000018 argV - tRNA-Arg (CCG) argV, tRNA-Arg, anticodon ccg, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 1512728 1512811 . + 0 MTB000022 leuW - tRNA-Leu (TAG) leuW, tRNA-Leu, anticodon tag, length = 84 No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 1828015 1828088 . - 0 MTB000023 leuV - tRNA-Leu (CAA) leuV, tRNA-Leu, anticodon caa, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 2256084 2256428 . - 0 Rv2007c fdxA Involved in electron transfer. Ferredoxin FdxA Rv2007c, (MTCY39.10), len: 114 aa. FdxA, ferredoxin, similar to many e.g. FER_MYCSM P00215 ferredoxin, Mycobacterium smegmatis (106 aa). P9WNE7 No intermediary metabolism and respiration P64122 P9WNE7 GO:0051539,GO:0009055,GO:0022900,GO:0046872,GO:0006810,GO:0051538 P9WNE7 NC_000962.3 Mycobrowser_v5 CDS 3872617 3873405 . + 0 Rv3451 cut3 Hydrolysis of cutin (a polyester that forms the structure of plant cuticle). Shown to have TDM-specific hydrolase activity (see Yang et al. 2014). Probable cutinase precursor Cut3 Rv3451, (MTCY13E12.04), len: 262 aa. Probable cut3, cutinase precursor, similar to others e.g. Q9KK87 from Mycobacterium avium (220 aa), FASTA scores: opt: 540, E(): 3.5e-24, (43.4% identity in 219 aa overlap); Q00298|CUTI_BOTCI|CUTA from Botrytis cinerea (Botryotinia fuckeliana) (202 aa), FASTA scores: opt: 214, E(): 2e-05, (31.45% identity in 210 aa overlap); Q9Y7G8 from Pyrenopeziza brassicae (203 aa), FASTA scores: opt: 203, E(): 8.5e-05, (31.05% identity in 190 aa overlap); P29292|CUTI_ASCRA from Ascochyta rabiei (223 aa), FASTA scores: opt: 155, E(): 0.054, (31.65% identity in 120 aa overlap). Similar to other proteins from Mycobacterium tuberculosis e.g. the downstream ORF O06319|Rv3452|MTCY13E12.05 hypothetical 23.1 KDA protein (226 aa), FASTA scores: opt: 775, E(): 1e-37, (58.65% identity in 220 aa overlap); Q50664|CUT2_MYCTU|Rv2301|MT2358|MTCY339.08c probable cutinase precursor (219 aa), FASTA scores: opt: 565, E(): 1.3e-25, (44.85% identity in 223 aa overlap); Q10837|CUT1_MYCTU|Rv1984c|MT2037|MTCY39.35 probable cutinase precursor (217 aa), FASTA scores: opt: 489, E(): 3e-21, (47.05% identity in 221 aa overlap); etc. Equivalent to AAK47897 from Mycobacterium tuberculosis strain CDC1551 (247 aa) but longer 15 aa. Contains cutinase, serine active site motif (PS00155). Belongs to the cutinase family. Alternative start possible at 3733. Start changed since first submission (+15 aa). P9WP39 No cell wall and cell processes P0A536 P9WP39 GO:0005576,GO:0008152,GO:0050525 3.1.1.74 P9WP39 NC_000962.3 Mycobrowser_v5 CDS 3873452 3874132 . + 0 Rv3452 cut4 Hydrolysis of cutin (a polyester that forms the structure of plant cuticle). Shown to have esterase and phospholipase activity. Shown to have TDM-specific hydrolase activity (see Yang et al. 2014). Probable cutinase precursor Cut4 Rv3452, (MTCY13E12.05), len: 226 aa. Probable cut4, cutinase precursor, similar to other e.g. Q9KK87 from Mycobacterium avium (220 aa), FASTA scores: opt: 522, E(): 7.3e-24, (46.6% identity in 221 aa overlap); P30272|CUTI_MAGGR|CUT1 from Magnaporthe grisea (Rice blast fungus) (Pyricularia grisea) (228 aa), FASTA scores: opt: 205, E(): 3.8e-05, (29.25% identity in 164 aa overlap); Q00298|CUTI_BOTCI|CUTA from Botrytis cinerea (Botryotinia fuckeliana) (202 aa), FASTA scores: opt: 204, E(): 3.9e-05, (33.5% identity in 209 aa overlap); etc. Similar to other proteins from Mycobacterium tuberculosis e.g. upstream ORF O06318|CUT3_MYCTU|Rv3451|MT3557|MTCY13E12.04 probable cutinase precursor (247 aa), FASTA scores: opt: 773, E(): 1.3e-38, (59.35% identity in 209 aa overlap); Q50664|CUT2_MYCTU|Rv2301|MT2358|MTCY339.08c probable cutinase precursor (219 aa), FASTA scores: opt: 704, E(): 1.3e-34, (53.4% identity in 219 aa overlap); etc. Contains PS00155 Cutinase, serine active site. Belongs to the cutinase family. Alternative start possible at 4553 in cSCY13E12 but no RBS. O06319 No cell wall and cell processes O06319 O06319 GO:0008152,GO:0016787 3.1.1.- O06319 NC_000962.3 Mycobrowser_v5 CDS 2979326 2979688 . - 0 Rv2658c Rv2658c Unknown Possible prophage protein Rv2658c, (MTCY441.27c), len: 120 aa. Hypothetical unknown protein, probably phage protein. P9WL47 No insertion seqs and phages P9WL47 NC_000962.3 Mycobrowser_v5 CDS 2939959 2940612 . - 0 Rv2612c pgsA1 Catalyzes the transfer of a free alcohol (inositol) onto CDP-diacylglycerol. The product of this putative ORF seems be essential to mycobacteria [catalytic activity: CDP-diacylglycerol + myo-inositol = CMP + phosphatidyl 1D-myo-inositol]. PgsA1 utilises inositol-phosphate rather than inositol, in contrast to its Eukaryotic ortholog (see Gräve et al. 2019). PI synthase PgsA1 (phosphatidylinositol synthase) (CDP-diacylglycerol--inositol-3-phosphatidyltransferase) Rv2612c, (MTCY01A10.21), len: 217 aa. pgsA1 (previously known as pgsA), PI synthase/CDP-diacylglyceride--inositol phosphatidyltransferase, transmembrane protein, equivalent to O07149|MLCL581.16c|PGSA|ML0454 putative phosphatidyltransferase from Mycobacterium leprae (239 aa), FASTA scores: opt: 1141, E(): 4.1e-70, (79.35% identity in 213 aa overlap); and Q9F7Y9|PGSA phosphatidylinositol synthase from Mycobacterium smegmatis (222 aa), FASTA scores: opt: 981, E(): 2.7e-59, (67.3% identity in 217 aa overlap) (see citation below). Also similar to other proteins e.g. Q9L282|SCL2.17c putative membrane transferase from Streptomyces coelicolor (241 aa), FASTA scores: opt: 564, E(): 4.9e-31, (43.4% identity in 212 aa overlap); Q9UYD0|PGSA-like|PAB1041 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase from Pyrococcus abyssi (186 aa), FASTA scores: opt: 264, E(): 8.4e-11, (33.15% identity in 190 aa overlap); Q9HQS2|PGSA|VNG1030G CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase from Halobacterium sp. strain NRC-1 (199 aa), FASTA scores: opt: 249, E(): 9.1e-10, (32.1% identity in 193 aa overlap); etc. Contains PS00379 CDP-alcohol phosphatidyltransferases signature. Belongs to the CDP-alcohol phosphatidyltransferase class-I family. Note that in Mycobacterium smegmatis, the psgA homologue is essential to the survival of the bacteria and seems cannot be compensated by any other enzyme of Mycobacterium smegmatis. P9WPG7 No lipid metabolism Q7D6W6 P9WPG7 GO:0016021,GO:0008654,GO:0005886,GO:0003881 2.7.8.11 P9WPG7 NC_000962.3 Mycobrowser_v5 CDS 1950632 1951051 . - 0 Rv1724c Rv1724c Unknown Hypothetical protein Rv1724c, (MTCY04C12.09c), len: 139 aa. Hypothetical unknown protein. P71982 No conserved hypotheticals P71982 NC_000962.3 Mycobrowser_v5 CDS 2006636 2006947 . + 0 Rv1772 Rv1772 Unknown Hypothetical protein Rv1772, (MTCY28.38), len: 103 aa. Hypothetical unknown protein. O06805 No conserved hypotheticals O06805 O06805 INH NC_000962.3 Mycobrowser_v5 CDS 2012081 2012530 . - 0 Rv1778c Rv1778c Unknown Unknown protein Rv1778c, (MTCY25C11.05c), len: 149 aa. Unknown protein. O33181 No conserved hypotheticals O33181 NC_000962.3 Mycobrowser_v5 CDS 2159921 2160328 . - 0 Rv1914c Rv1914c Unknown Unknown protein Rv1914c, (MTCY180.04), len: 135 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). O07719 No conserved hypotheticals O07719 NC_000962.3 Mycobrowser_v5 CDS 2202584 2203129 . + 0 Rv1957 Rv1957 Unknown Hypothetical protein Rv1957, (MTCY09F9.07c), len: 181 aa. Hypothetical unknown protein. P95257 No conserved hypotheticals P95257 P95257 NC_000962.3 Mycobrowser_v5 CDS 2280240 2281082 . - 0 Rv2033c Rv2033c Unknown Conserved hypothetical protein Rv2033c, (MTV018.20), len: 280 aa. Conserved hypothetical protein, similar to many. O53477 No conserved hypotheticals O53477 O53477 NC_000962.3 Mycobrowser_v5 CDS 2287884 2288681 . - 0 Rv2042c Rv2042c Unknown Conserved protein Rv2042c, (MTV018.29c), len: 265 aa. Conserved protein, similar to many. O53486 No conserved hypotheticals O53486 O53486 GO:0006810,GO:0005622 O53486 NC_000962.3 Mycobrowser_v5 CDS 2327491 2328225 . - 0 Rv2070c cobK Involved in cobalamin biosynthesis. Precorrin-6X reductase CobK Rv2070c, (MTCY49.09c), len: 244 aa. CobK, precorrin-6x reductase, similar to many. P9WP89 No intermediary metabolism and respiration Q10680 P9WP89 GO:0055114,GO:0016994,GO:0009236 1.3.1.54 P9WP89 NC_000962.3 Mycobrowser_v5 CDS 2402507 2402722 . - 0 Rv2142A parD2 Unknown Possible antitoxin ParD2 Rv2142A, len: 71 aa. Possible parD2, antitoxin, part of toxin-antitoxin (TA) operon with Rv2142c (See Pandey and Gerdes, 2005). P9WJ75 No virulence, detoxification, adaptation P9WJ75 NC_000962.3 Mycobrowser_v5 tRNA 2835494 2835566 . + 0 MTB000034 lysU - tRNA-Lys (CTT) lysU, tRNA-Lys, anticodon ctt, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 2881252 2881320 . + 0 MTB000068 mpr11 Unknown Fragment of putative small regulatory RNA mpr11, fragment of putative small regulatory RNA (See DiChiara et al., 2010), ends not mapped, 82-100 nt band detected by Northern blot in M. bovis BCG Pasteur. No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 1138076 1138147 . - 0 MTB000016 glnT - tRNA-Gln (TTG) glnT, tRNA-Gln, anticodon ttg, length = 72 No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 1177396 1177469 . + 0 MTB000017 leuX - tRNA-Leu (TAA) leuX, tRNA-Leu, anticodon taa, length = 74. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1220388 1220487 . - 0 MTB000074 MTS0858 Unknown Putative small regulatory RNA MTS0858, putative small regulatory RNA (See Arnvig et al., 2011), ends not mapped, ~100 bp band detected by Northern blot. No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 1535417 1535716 . - 0 MTB000065 mcr15 Unknown Putative small regulatory RNA mcr15, putative small regulatory RNA (See DiChiara et al., 2010). 5'-end mapped by 5'RLM-RACE in M. bovis BGC Pasteur, 3'-end not mapped. No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 2882185 2882276 . + 0 MTB000069 mpr12 Unknown Fragment of putative small regulatory RNA mpr12, fragment of putative small regulatory RNA (See DiChiara et al., 2010), ends not mapped, ~118 nt band detected by Northern blot in M. bovis BCG Pasteur. No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 2765331 2765404 . - 0 MTB000030 proU - tRNA-Pro (TGG) proU, tRNA-Pro; anticodon tgg, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 3020200 3020457 . - 0 Rv2706c Rv2706c Unknown Hypothetical protein Rv2706c, (MTCY05A6.27c), len: 85 aa (unlikely ORF). Hypothetical unknown protein. O07207 No conserved hypotheticals O07207 NC_000962.3 Mycobrowser_v5 CDS 3042542 3043018 . + 0 Rv2730 Rv2730 Unknown Hypothetical protein Rv2730, (MTCY174.10), len: 158 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6Y1K4 No conserved hypotheticals I6Y1K4 NC_000962.3 Mycobrowser_v5 CDS 3115408 3115719 . + 0 Rv2809 Rv2809 Unknown Hypothetical protein Rv2809, (MTCY16B7.34c), len: 103 aa (questionable ORF). Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6YEF3 No conserved hypotheticals I6YEF3 I6YEF3 NC_000962.3 Mycobrowser_v5 CDS 2926355 2926975 . + 0 Rv2597 Rv2597 Unknown Probable membrane protein Rv2597, (MTCY227.04c), len: 206 aa. Probable membrane protein. Predicted to be an outer membrane protein (See Song et al., 2008). P9WL73 No cell wall and cell processes P9WL73 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2962470 2962712 . + 0 Rv2635 Rv2635 Unknown Hypothetical protein Rv2635, (MTCY441.05), len: 80 aa. Hypothetical unknown protein. P9WL57 No conserved hypotheticals P9WL57 NC_000962.3 Mycobrowser_v5 CDS 2976586 2976909 . - 0 Rv2653c Rv2653c Unknown Possible PhiRv2 prophage protein Rv2653c, (MTCY441.22c), len: 107 aa. Hypothetical unknown protein, possibly phiRv2 phage protein (see citation below). P9WJ13 No insertion seqs and phages P9WJ13 NC_000962.3 Mycobrowser_v5 CDS 3366644 3367267 . + 0 Rv3008 Rv3008 Unknown Hypothetical protein Rv3008, (MTV012.22), len: 207 aa (start uncertain). Hypothetical unknown protein. I6YEY1 No conserved hypotheticals I6YEY1 NC_000962.3 Mycobrowser_v5 CDS 3392812 3393201 . + 0 Rv3032A Rv3032A Unknown Conserved protein Rv3032A, len: 129 aa. Conserved protein. I6X630 No conserved hypotheticals I6X630 NC_000962.3 Mycobrowser_v5 CDS 3540882 3541364 . - 0 Rv3172c Rv3172c Unknown Hypothetical protein Rv3172c, (MTV014.16c), len: 160 aa. Hypothetical unknown protein. O53322 No conserved hypotheticals O53322 O53322 NC_000962.3 Mycobrowser_v5 CDS 3611300 3611941 . + 0 Rv3235 Rv3235 Unknown Hypothetical alanine arginine proline rich protein Rv3235, (MTCY20B11.10), len: 213 aa. Hypothetical unknown ala-, arg-, pro-rich protein. O05880 No conserved hypotheticals O05880 O05880 NC_000962.3 Mycobrowser_v5 CDS 3908072 3908236 . + 0 Rv3489 Rv3489 Unknown Unknown protein Rv3489, (MTCY13E12.42), len: 54 aa. Unknown protein. No similarity with other proteins. I6YC91 No conserved hypotheticals I6YC91 I6YC91 NC_000962.3 Mycobrowser_v5 CDS 3909890 3910468 . + 0 Rv3491 Rv3491 Unknown Unknown protein Rv3491, (MTCY13E12.44), len: 192 aa. Unknown protein. No significant homology with other proteins. I6XHD1 No conserved hypotheticals I6XHD1 NC_000962.3 Mycobrowser_v5 CDS 3963605 3964054 . + 0 Rv3527 Rv3527 Unknown Hypothetical protein Rv3527, (MTCY03C7.29c), len: 149 aa. Hypothetical unknown protein. I6XHG6 No conserved hypotheticals I6XHG6 NC_000962.3 Mycobrowser_v5 CDS 4196724 4197107 . + 0 Rv3747 Rv3747 Function unknown Conserved protein Rv3747, (MTV025.095), len: 127 aa. Conserved protein, highly similar to downstream ORF O69715|Rv3748|MTV025.096 conserved hypothetical protein (119 aa), FASTA scores: opt: 494, E(): 6e-27, (64.4% identity in 118 aa overlap). O69714 No conserved hypotheticals O69714 NC_000962.3 Mycobrowser_v5 tRNA 2765541 2765611 . + 0 MTB000031 glyV - tRNA-Gly (TCC) glyV, tRNA-Gly; anticodon tcc, length = 71 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 3174747 3174995 . + 0 Rv2862A vapB23 Unknown Possible antitoxin VapB23 Rv2862A, len: 82 aa. Possible vapB23, antitoxin, part of toxin-antitoxin (TA) operon with Rv2863 (See Pandey and Gerdes, 2005). P0CW32 No virulence, detoxification, adaptation P0CW32 NC_000962.3 Mycobrowser_v5 CDS 4008167 4008433 . - 0 Rv3566A Rv3566A Unknown Hypothetical protein Rv3566A, len: 88 aa. Hypothetical unknown protein. I6YGI1 No conserved hypotheticals I6YGI1 NC_000962.3 Mycobrowser_v5 CDS 4042952 4043035 . - 0 Rv3599c Rv3599c Unknown Hypothetical short protein Rv3599c, (MTCY07H7B.23), len: 27 aa. Hypothetical unknown protein. O06283 No unknown O06283 NC_000962.3 Mycobrowser_v5 tRNA 3431840 3431912 . - 0 MTB000041 alaU - tRNA-Ala (GGC) alaU, tRNA-Ala, anticodon ggc, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 4081365 4081437 . - 0 MTB000044 thrU - tRNA-Thr (CGT) thrU, tRNA-Thr, anticodon cgt, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 2772098 2772331 . - 0 Rv2468A Rv2468A Unknown Conserved protein Rv2468A, len: 77 aa. Conserved protein. I6YDH3 No conserved hypotheticals I6YDH3 NC_000962.3 Mycobrowser_v5 CDS 4053881 4054042 . - 0 Rv3613c Rv3613c Unknown Hypothetical protein Rv3613c, (MTCY07H7B.09), len: 53 aa. Hypothetical unknown protein. O06270 No conserved hypotheticals O06270 NC_000962.3 Mycobrowser_v5 CDS 3708074 3708316 . - 0 Rv3321c vapB44 Unknown Possible antitoxin VapB44 Rv3321c, (MTV016.21c), len: 80 aa. Possible vapB44, antitoxin, part of toxin-antitoxin (TA) operon with Rv0299, see Arcus et al. 2005. Similar to several others in Mycobacterium tuberculosis (strains H37Rv and CDC1551) e.g. AAK48167|MT3800 DNA-binding protein (COPG family) from strain CDC1551 (74 aa), FASTA scores: opt: 142, E(): 0.0016, (48.85% identity in 43 aa overlap); AAK46916|MT2606 hypothetical 8.0 KDA protein from strain CDC1551 (74 aa), FASTA scores: opt: 139, E(): 0.0026, (37.2% identity in 78 aa overlap); O50456|Rv1241|MTV006.13 hypothetical 9.9 KDA protein from strain H37Rv (86 aa), FASTA scores: opt: 134, E(): 0.0066, (39.0% identity in 82 aa overlap); etc. P9WJ17 No virulence, detoxification, adaptation O53373 P9WJ17 GO:0006355,GO:0003677 NC_000962.3 Mycobrowser_v5 CDS 3598051 3598356 . - 0 Rv3221A rshA Binds sigma factor sigh and inhibits it. Probably involved in survival following heat shock and oxidative stress. Anti-sigma factor RshA Rv3221A, len: 101 aa. RshA, anti-sigma factor, similar to Q9XCD7|AAD41811.1 unknown protein from Mycobacterium smegmatis, linked to sigma factor sigH (see Fernandes et al., 1999) (101 aa), FASTA scores: opt: 422, E(): 3.4e-22, (64.9% identity in 94 aa overlap); and to Q9RL96|RsrA anti-sigma factor from Streptomyces coelicolor (see Kang et al., 1999) (105 aa), FASTA scores: opt: 163, E(): 0.00016, (32.05% identity in 78 aa overlap). P9WJ69 No information pathways P9WJ69 NC_000962.3 Mycobrowser_v5 CDS 3879273 3879692 . - 0 Rv3459c rpsK S11 plays an essential role for the selection of the correct tRNA in protein biosynthesis. It is located on the large lobe of the small subunit. 30S ribosomal protein S11 RpsK Rv3459c, (MTCY13E12.12c), len: 139 aa. rpsK, 30S ribosomal protein S11, equivalent to Q9X7A0|RS11_MYCLE|RPSK|ML1959|MLCB1222.29c 30S ribosomal protein S11 from Mycobacterium leprae (138 aa), FASTA scores: opt: 819, E(): 7.6e-44, (89.95% identity in 139 aa overlap); and P45812|RS11_MYCBO 30S ribosomal protein S11 from Mycobacterium bovis (139 aa), FASTA scores: opt: 867, E(): 8.4e-47, (94.25% identity in 139 aa overlap). Also highly similar to others e.g. P72403|RS11_STRCO|SC6G4.06 from Streptomyces coelicolor (134 aa), FASTA scores: opt: 729, E(): 2.6e-38, (79.85% identity in 139 aa overlap); O50633|RS11_BACHD|RPSK|BH0161 from Bacillus halodurans (129 aa), FASTA scores: opt: 618, E(): 1.7e-31, (70.3% identity in 128 aa overlap); P04969|RS11_BACSU|RPSK from Bacillus subtilis (131 aa), FASTA scores: opt: 601, E(): 2e-30, (69.0% identity in 129 aa overlap); etc. Contains ribosomal protein S11 signature (PS00054). Belongs to the S11P family of ribosomal proteins. P9WH65 No information pathways O06326 P9WH65 GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WH65 NC_000962.3 Mycobrowser_v5 ncRNA 4099386 4099478 . - 0 MTB000058 B11 Unknown Putative small regulatory RNA B11, putative small regulatory RNA (See Arnvig and Young, 2009; DiChiara et al., 2010). No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 547344 547517 . - 0 Rv0456B mazE1 Unknown Possible antitoxin MazE1 Rv0456B, len: 57 aa. Possible mazE1, antitoxin, part of toxin-antitoxin (TA) operon with Rv0456A (See Pandey and Gerdes, 2005; Zhu et al., 2006). P0CL57 No virulence, detoxification, adaptation P0CL57 NC_000962.3 Mycobrowser_v5 CDS 883451 884050 . - 0 Rv0789c Rv0789c Unknown Hypothetical protein Rv0789c, (MTCY369.33c), len: 199 aa. Hypothetical unknown protein. I6Y4U0 No conserved hypotheticals I6Y4U0 I6Y4U0 NC_000962.3 Mycobrowser_v5 CDS 3533913 3534395 . - 0 Rv3165c Rv3165c Unknown Unknown protein Rv3165c, (MTV014.09)c, len: 160 aa. Unknown protein. O53315 No conserved hypotheticals O53315 NC_000962.3 Mycobrowser_v5 CDS 474940 475344 . + 0 Rv0395 Rv0395 Unknown Hypothetical protein Rv0395, (MTCY04D9.07), len: 134 aa. Hypothetical unknown protein. P95203 No conserved hypotheticals P95203 NC_000962.3 Mycobrowser_v5 tRNA 658109 658189 . + 0 MTB000005 tyrT - tRNA-Tyr (GTA) tyrT, tRNA-Tyr, anticodon gta, length = 81 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 4013511 4014041 . + 0 Rv3572 Rv3572 Unknown Unknown protein Rv3572, (MTCY06G11.19), len: 176 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). I6X7P2 No conserved hypotheticals I6X7P2 NC_000962.3 Mycobrowser_v5 tRNA 733524 733596 . + 0 MTB000008 trpT - tRNA-Trp (CCA) trpT, tRNA-Trp, anticodon cca, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 1113511 1113583 . + 0 MTB000015 alaV - tRNA-Ala (CGC) alaV, tRNA-Ala, anticodon cgc, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 376573 377055 . - 0 Rv0307c Rv0307c Unknown Unknown protein Rv0307c, (MTCY63.12c), len: 160 aa. Unknown protein. O07234 No conserved hypotheticals O07234 O07234 NC_000962.3 Mycobrowser_v5 CDS 665042 665383 . - 0 Rv0572c Rv0572c Unknown Hypothetical protein Rv0572c, (MTV039.10c), len: 113 aa. Hypothetical unknown protein. P9WM81 No conserved hypotheticals P9WM81 NC_000962.3 Mycobrowser_v5 CDS 1280843 1281484 . - 0 Rv1154c Rv1154c Unknown Hypothetical protein Rv1154c, (MTCI65.21c), len: 213 aa. Hypothetical unknown protein, start uncertain. O06552 No conserved hypotheticals O06552 O06552 O06552 NC_000962.3 Mycobrowser_v5 CDS 2075571 2075828 . + 0 Rv1831 Rv1831 Unknown Hypothetical protein Rv1831, (MTCY1A11.12c), len: 85 aa. Hypothetical unknown protein. P9WLR3 No conserved hypotheticals P9WLR3 P9WLR3 NC_000962.3 Mycobrowser_v5 CDS 1735976 1736212 . + 0 Rv1535 Rv1535 Unknown Unknown protein Rv1535, (MTCY07A7A.04), len: 78 aa. Unknown protein. O06180 No conserved hypotheticals O06180 NC_000962.3 Mycobrowser_v5 CDS 2314661 2314825 . - 0 Rv2057c rpmG1 Involved in translation 50S ribosomal protein L33 RpmG1 Rv2057c, (MTCY63A.03), len: 54 aa. rpmG1, 50S ribosomal protein L33, similar to many. Note that previously known as rpmG. P9WH97 No information pathways P0A5W0 P9WH97 GO:0003735,GO:0006412,GO:0005840 P9WH97 NC_000962.3 Mycobrowser_v5 CDS 1612256 1612393 . + 0 Rv1434 Rv1434 Unknown Hypothetical protein Rv1434, (MTCY493.20c), len: 45 aa. Hypothetical unknown protein. O06824 No conserved hypotheticals O06824 NC_000962.3 Mycobrowser_v5 CDS 2201277 2201579 . + 0 Rv1954A Rv1954A Unknown Hypothetical protein Rv1954A, len: 100 aa. Hypothetical unknown protein. P0CV86 No unknown P0CV86 NC_000962.3 Mycobrowser_v5 tRNA 1946613 1946686 . + 0 MTB000024 proT - tRNA-Pro (GGG) proT, tRNA-Pro, anticodon ggg, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 2139419 2139656 . + 0 MTB000056 AS1890 Unknown Putative small regulatory RNA AS1890, putative small regulatory RNA (See Arnvig and Young, 2009). Alternate 5'-ends at positions 2139466, 2139548, 2139594. No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 2510598 2510669 . - 0 MTB000027 valV - tRNA-Val (TAC) valV, tRNA-Val, anticodon tac, length = 72 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 3082352 3082756 . - 0 Rv2774c Rv2774c Unknown Hypothetical protein Rv2774c, (MTV002.39c), len: 134 aa. Hypothetical unknown protein. O33316 No conserved hypotheticals O33316 O33316 NC_000962.3 Mycobrowser_v5 CDS 3502945 3503277 . - 0 Rv3136A Rv3136A Unknown Conserved protein Rv3136A, len: 110 aa. Conserved protein. I6Y2Q7 No conserved hypotheticals I6Y2Q7 I6Y2Q7 NC_000962.3 Mycobrowser_v5 CDS 3556855 3557064 . + 0 Rv3190A Rv3190A Unknown Conserved protein Rv3190A, len: 69 aa. Conserved protein. I6XGJ1 No conserved hypotheticals I6XGJ1 NC_000962.3 Mycobrowser_v5 CDS 3566696 3566896 . - 0 Rv3196A Rv3196A Unknown Unknown protein Rv3196A, len: 66 aa. Unknown protein. L7N668 No conserved hypotheticals L7N668 NC_000962.3 Mycobrowser_v5 tRNA 4168345 4168430 . + 0 MTB000046 serV - tRNA-Ser (GGA) serV, tRNA-Ser, anticodon gga, length = 86 No stable RNAs NC_000962.3 Mycobrowser_v5 ncRNA 4317073 4317165 . + 0 MTB000080 MTS2975 Unknown Putative small regulatory RNA MTS2975, putative small regulatory RNA (See Arnvig et al., 2011), ends not mapped, ~100 bp band detected by Northern blot. No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 1774860 1775144 . - 0 Rv1567c Rv1567c Unknown Probable hypothetical membrane protein Rv1567c, (MTCY336.36), len: 94 aa. Probable membrane protein. O06623 No cell wall and cell processes O06623 NC_000962.3 Mycobrowser_v5 CDS 1815253 1815693 . + 0 Rv1615 Rv1615 Unknown Probable membrane protein Rv1615, (MTCY01B2.07), len: 146 aa. Probable membrane protein O06132 No cell wall and cell processes O06132 O06132 NC_000962.3 Mycobrowser_v5 CDS 1786307 1786528 . - 0 Rv1584c Rv1584c Unknown Possible PhiRv1 phage protein Rv1584c, (MTCY336.20), len: 73 aa. Possible phiRv1 phage protein (putative excisionase) (see citation below). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06606 No insertion seqs and phages O06606 NC_000962.3 Mycobrowser_v5 rRNA 1471846 1473382 . + 0 MTB000019 rrs - Ribosomal RNA 16S rrs, 16s rRNA gene (alternate gene name: rrnS). No stable RNAs AMI,SM NC_000962.3 Mycobrowser_v5 CDS 606149 606448 . + 0 Rv0514 Rv0514 Unknown Possible transmembrane protein Rv0514, (MTCY20G10.04), len: 99 aa. Possible transmembrane protein. O33359 No cell wall and cell processes O33359 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 710345 710587 . + 0 Rv0615 Rv0615 Unknown Probable integral membrane protein Rv0615, (MTCY19H5.06c), len: 80 aa. Probable integral membrane protein. O07762 No cell wall and cell processes O07762 NC_000962.3 Mycobrowser_v5 CDS 789157 789411 . - 0 Rv0689c Rv0689c Unknown Hypothetical protein Rv0689c, (MTCY210.06c), len: 84 aa. Hypothetical unknown protein. I6WZ39 No conserved hypotheticals I6WZ39 NC_000962.3 Mycobrowser_v5 tRNA 386204 386274 . - 0 MTB000004 glyU - tRNA-Gly (CCC) glyU, tRNA-Gly, anticodon ccc, length = 71 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 2546839 2547087 . - 0 Rv2274A mazE8 Unknown Possible antitoxin MazE8 Rv2274A, len: 82 aa. Possible mazE8, antitoxin, part of toxin-antitoxin (TA) operon with Rv2274c (See Pandey and Gerdes, 2005). P0CL60 No virulence, detoxification, adaptation P0CL60 NC_000962.3 Mycobrowser_v5 CDS 1003805 1003957 . + 0 Rv0900 Rv0900 Unknown Possible membrane protein Rv0900, (MTCY31.28), len: 50 aa. Possible membrane protein, with hydrophobic domain from aa 4-26. P9WJG7 No cell wall and cell processes P9WJG7 NC_000962.3 Mycobrowser_v5 CDS 2307293 2307517 . - 0 Rv2049c Rv2049c Unknown Conserved hypothetical protein Rv2049c, (MTV018.36c), len: 74 aa. Conserved hypothetical protein. O53491 No conserved hypotheticals O53491 NC_000962.3 Mycobrowser_v5 tRNA 850642 850713 . - 0 MTB000009 thrV - tRNA-Thr (TGT) thrV, tRNA-Thr, anticodon tgt, length = 72 No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 3348547 3348619 . - 0 MTB000039 gluU - tRNA-Glu (CTC) gluU, tRNA-Glu; anticodon ctc, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 186495 186623 . - 0 Rv0157A Rv0157A Unknown Conserved protein Rv0157A, len: 42 aa. Conserved protein, showing similarity to C-terminal part (aa 186-220) of O53976|Rv1975|MTV051.13 conserved hypothetical protein from Mycobacterium tuberculosis (221 aa), FASTA scores: opt: 173, E(): 3e-06, (62.5% identity in 40 aa overlap). I6WXK8 No conserved hypotheticals I6WXK8 NC_000962.3 Mycobrowser_v5 CDS 28362 29207 . + 0 Rv0024 Rv0024 Unknown. The P60 protein is a major extracellular protein may be involved in the invasion of host cells. Putative secreted protein P60-related protein Rv0024, (MTCY10H4.24), len: 281 aa. Putative secreted protein, p60 homologue, similar to many. Similar to Mycobacterium tuberculosis proteins Rv1477, Rv1478, Rv1566c, Rv2190c. Could belong to the E. coli NLPC / listeria P60 family. P71594 No virulence, detoxification, adaptation P71594 P71594 P71594 NC_000962.3 Mycobrowser_v5 CDS 1947416 1947643 . - 0 Rv1721c vapB12 Unknown Possible antitoxin VapB12 Rv1721c, (MTCY04C12.06c), len: 75 aa. Possible vapB12, antitoxin, part of toxin-antitoxin (TA) operon with Rv1720c (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others from Mycobacterium tuberculosis e.g. Rv0300|MTCY63.05|O07227 conserved hypothetical protein (73 aa). Start changed since original submission. P9WJ53 No virulence, detoxification, adaptation P9WJ53 P9WJ53 NC_000962.3 Mycobrowser_v5 CDS 4434 4997 . + 0 Rv0004 Rv0004 Function unknown Conserved hypothetical protein Rv0004, (MTCY10H4.02), len: 187 aa. Conserved hypothetical protein (see Salazar et al., 1996). Belongs to superfamily DUF721; this family contains several actinomycete proteins of unknown function. P9WFL1 No conserved hypotheticals P71573 P9WFL1 NC_000962.3 Mycobrowser_v5 CDS 2337306 2337869 . + 0 Rv2080 lppJ Unknown Lipoprotein LppJ Rv2080, (MTCY49.19), len: 187 aa. LppJ, lipoprotein; contains prokayotic lipoprotein modification site (PS00013) and signal sequence at N-terminus. P9WK77 No cell wall and cell processes P9WK77 GO:0005886 NC_000962.3 Mycobrowser_v5 CDS 42433 43365 . - 0 Rv0040c mtc28 Unknown Secreted proline rich protein Mtc28 (proline rich 28 kDa antigen) Rv0040c, (MTCY21D4.03c), len: 310 aa. Mtc28, secreted proline rich 28 kDa antigen protein (has hydrophobic stretch at N-terminus) (see citation below). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WIM9 No cell wall and cell processes P0A5Q6 P9WIM9 P9WIM9 NC_000962.3 Mycobrowser_v5 CDS 31514 31819 . + 0 Rv0028 Rv0028 Unknown Conserved hypothetical protein Rv0028, (MTCY10H4.28), len: 101 aa. Conserved hypothetical unknown protein. P9WM97 No conserved hypotheticals P9WM97 P9WM97 NC_000962.3 Mycobrowser_v5 ncRNA 1471619 1471742 . + 0 MTB000064 mcr3 Unknown Putative small regulatory RNA mcr3, putative small regulatory RNA (See DiChiara et al., 2010). 5'-end mapped by 5'RLM-RACE in M. bovis BGC Pasteur, 3'-end not mapped. No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 33224 33553 . + 0 Rv0030 Rv0030 Unknown Conserved hypothetical protein Rv0030, (MTCY10H4.30), len: 109 aa. Conserved hypothetical unknown protein. P9WM95 No conserved hypotheticals P9WM95 NC_000962.3 Mycobrowser_v5 CDS 33582 33794 . + 0 Rv0031 Rv0031 Normally, required for the transposition of an insertion element. Possible remnant of a transposase Rv0031, (MTCY10H4.31), len: 70 aa. Possible remnant of a transposase, showing partial similarity to mycobacterial transposases in a short overlap, e.g. Rv2791c|MTV002_57 (459 aa), FASTA score: (72.2% identity in 36 aa overlap); Rv2885c, Rv2978c, Rv3827c, etc. P71601 No insertion seqs and phages P71601 P71601 NC_000962.3 Mycobrowser_v5 CDS 51828 52697 . - 0 Rv0048c Rv0048c Unknown Possible membrane protein Rv0048c, MTCY21D4.11c, len: 289 aa. Possible membrane protein. P9WM87 No cell wall and cell processes P71705 P9WM87 GO:0005886,GO:0016021 P9WM87 NC_000962.3 Mycobrowser_v5 rRNA 1476899 1477013 . + 0 MTB000021 rrf - Ribosomal RNA 5S rrf, 5S rRNA gene. Identical to Em_ba:MT5SRR, D10035 M.tuberculosis 5S rRNA, len: 116. No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 86528 87133 . + 0 Rv0078 Rv0078 Possibly involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv0078, (MTV030.22), len: 201 aa. Probable transcriptional regulator. Contains probable helix-turn-helix motif from aa 35 to 56 (Score 1348, +3.78 SD). O53623 No regulatory proteins O53623 O53623 GO:0006350,GO:0003700,GO:0006355 O53623 NC_000962.3 Mycobrowser_v5 tRNA 10887 10960 . + 0 MTB000001 ileT - tRNA-Ile (GAT) ileT, tRNA-Ile, anticodon gat, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 11112 11184 . + 0 MTB000002 alaT - tRNA-Ala (TGC) alaT, tRNA-Ala, anticodon tgc, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 2123174 2123611 . + 0 Rv1873 Rv1873 Unknown Conserved hypothetical protein Rv1873, (MTCY180.45c), len: 145 aa. Conserved hypothetical protein. Some similarity to AL591783 hypothetical protein from Sinorhizobium meliloti. O07756 No conserved hypotheticals 2JEK O07756 O07756 O07756 NC_000962.3 Mycobrowser_v5 CDS 1160095 1160433 . - 0 Rv1036c Rv1036c Possibly required for the transposition of the insertion element IS1560. Probable IS1560 transposase (fragment) Rv1036c, (MTCY10G2.13), len: 112 aa. Probable IS1560 transposase fragment, similar to part of Rv3386|E1202304|MTV004.44 (234 aa) (82.8% identity in 87 aa overlap). P96365 No insertion seqs and phages P96365 P96365 NC_000962.3 Mycobrowser_v5 CDS 882524 882763 . + 0 Rv0787A Rv0787A Function unknown Conserved protein Rv0787A, len: 79 aa. Conserved protein, equivalent to MLCB5.24 hypothetical protein from Mycobacterium leprae (79 aa), FASTA scores: opt: 434, (84.8% identity in 79 aa overlap). Also similar to P12049|YEXA_BACSU hypothetical 9.7 kDa protein from Bacillus subtilis (84 aa), FASTA scores: opt: 172, E(): 4e-06, (44.4% identity in 72 aa overlap). Belongs to the UPF0062 family. I6Y8S6 No conserved hypotheticals Q8VKE0 I6Y8S6 GO:0016879 I6Y8S6 NC_000962.3 Mycobrowser_v5 CDS 2338065 2338505 . - 0 Rv2081c Rv2081c Unknown Conserved transmembrane protein Rv2081c, (MTCY49.20c), len: 146 aa. Conserved transmembrane protein, similar to many. Hydrophobic stretch from aa 32-54. P9WLK5 No cell wall and cell processes P9WLK5 GO:0005886,GO:0016021 P9WLK5 NC_000962.3 Mycobrowser_v5 CDS 1779194 1779298 . - 0 Rv1572c Rv1572c Unknown Conserved hypothetical protein Rv1572c, (MTCY336.31B), len: 34 aa. Partial ORF, part of REP13E12 repeat element; 3' end of Rv1587c (MTCY336.17) after phage-like element (see citation below). Similar to C-terminal ends of other REP13E12 repeat elements e.g. Rv1148, Rv1945, Rv3467, etc. Length extended since first submission (+7 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06618 No insertion seqs and phages O06618 NC_000962.3 Mycobrowser_v5 CDS 25913 26881 . - 0 Rv0021c Rv0021c Function unknown Conserved hypothetical protein Rv0021c, (MTCY10H4.21c), len: 322 aa. Conserved hypothetical protein, similar to various proteins. P71591 No conserved hypotheticals P71591 P71591 GO:0055114,GO:0018580 P71591 NC_000962.3 Mycobrowser_v5 CDS 476394 476642 . + 0 Rv0397A Rv0397A Unknown Conserved protein Rv0397A, len: 82 aa. Conserved protein. I6Y3N9 No conserved hypotheticals I6Y3N9 NC_000962.3 Mycobrowser_v5 CDS 1492320 1494425 . - 0 Rv1327c glgE Function unknown; probably involved in polysaccharides degradation. M1P-dependent maltosyltransferase GlgE Rv1327c, (MTCY130.12c), len: 701 aa. Probable glgE, glucanase, similar to AF172946|AF172946_2 putative glucanase GlgE from Mycobacterium smegmatis (697 aa), FASTA scores: opt: 3816, E(): 0, (78.5% identity in 692 aa overlap). Similar to putative alpha-amylases e.g. Q9L1K2 Streptomyces coelicolor (675 aa), FASTA scores: opt: 2243, E(): 7.4e-132, (54.2% identity in 684 aa overlap). Start changed since original submission (-36) based on similarity to GlgE of Mycobacterium smegmatis; previous start at position 1494531. P9WQ17 No intermediary metabolism and respiration P63531 P9WQ17 GO:0043169,GO:0016798,GO:0005975 3.2.1.- P9WQ17 NC_000962.3 Mycobrowser_v5 CDS 1 1524 . + 0 Rv0001 dnaA Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (DNAA box): 5'-TTATC(C/A)A(C/A)A-3'. DNAA binds to ATP and to acidic phospholipids. DNAA protein binds the origin of replication (oriC), ATP and ADP, and exhibited weak ATPase activity. Chromosomal replication initiator protein DnaA Rv0001, (MT0001, MTV029.01, P49993), len: 507 aa. dnaA, chromosomal replication initiator protein (see citations below), equivalent to other Mycobacterial chromosomal replication initiator proteins. Also highly similar to others except in N-terminus e.g. Q9ZH75|DNAA_STRCH chromosomal replication initiator protein from Streptomyces chrysomallus (624 aa). Contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS01008 DnaA protein signature. Belongs to the DnaA family. Note that the first base of this gene has been taken as base 1 of the Mycobacterium tuberculosis H37Rv genomic sequence. P9WNW3 No information pathways P49993 P9WNW3 GO:0003688,GO:0006270,GO:0005737,GO:0017111,GO:0006275,GO:0005524 P9WNW3 NC_000962.3 Mycobrowser_v5 CDS 2716395 2717138 . - 0 Rv2418c octT Transfers octanoate to glucosylglycerate (GG) and diglucosylglycerate (DGG), the earliest intermediates in methylglucose lipopolysaccharides (MGLP) biosynthesis (see Maranha et al. 2015). Sugar octanoyltransferase OctT Enzymatic studies, synthetic chemistry, NMR spectroscopy and mass spectrometry approaches suggest that, in contrast to the prevailing consensus, octanoate is not esterified to the primary hydroxyl group of glycerate but instead to the C6 OH of the second glucose in DGG (see Maranha et al. 2015). P71725 No conserved hypotheticals P71725 P71725 NC_000962.3 Mycobrowser_v5 CDS 2052 3260 . + 0 Rv0002 dnaN DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP-independent) along duplex DNA [catalytic activity: N deoxynucleoside triphosphate = N diphosphate + {DNA}N]. DNA polymerase III (beta chain) DnaN (DNA nucleotidyltransferase) Rv0002, (MTV029.02, MTCY10H4.0), len: 402 aa. DnaN, DNA polymerase III (beta chain) (see citations below), equivalent to other Mycobacterial DNA polymerases III beta chain. Also highly similar to others e.g. P27903|DP3B_STRCO DNA polymerase III beta chain from Streptomyces coelicolor (376 aa). Overlaps and extends CDS in neighbouring cosmid MTCY10H4.01. P9WNU1 No information pathways 3P16 Q50790 P9WNU1 GO:0003677,GO:0006260,GO:0003887,GO:0005737,GO:0008408 2.7.7.7 P9WNU1 NC_000962.3 Mycobrowser_v5 CDS 3280 4437 . + 0 Rv0003 recF The RECF protein is involved in DNA metabolism and recombination; it is required for DNA replication and normal sos inducibility. RECF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP. DNA replication and repair protein RecF (single-strand DNA binding protein) Rv0003, (MTCY10H4.01), len: 385 aa. RecF, DNA replication and repair protein (see citations below), equivalent to other mycobacterial DNA replication and repair proteins. Also highly similar to many others. Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00617 RecF protein signature 1, and PS00618 RecF protein signature 2. Belongs to the RecF family. P9WHI9 No information pathways Q59586 P9WHI9 GO:0006281,GO:0006260,GO:0009432,GO:0005737,GO:0003697,GO:0005524 P9WHI9 NC_000962.3 Mycobrowser_v5 CDS 7302 9818 . + 0 Rv0006 gyrA DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [catalytic activity: ATP-dependent breakage, passage and rejoining of double-stranded DNA]. DNA gyrase (subunit A) GyrA (DNA topoisomerase (ATP-hydrolysing)) (DNA topoisomerase II) (type II DNA topoisomerase) Rv0006, (MTCY10H4.04), len: 838 aa. GyrA, DNA gyrase subunit A (see citations below). Contains PS00018 EF-hand calcium-binding domain. P9WG47 No information pathways 3ILW,3IFZ Q07702 P9WG47 GO:0003918,GO:0006265,GO:0046677,GO:0005524 5.99.1.3 FLQ P9WG47 NC_000962.3 Mycobrowser_v5 CDS 9914 10828 . + 0 Rv0007 Rv0007 Unknown Possible conserved membrane protein Rv0007, (MTCY10H4.05), len: 304 aa. Possible conserved membrane protein. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WMA7 No cell wall and cell processes P71575 P9WMA7 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 11874 12311 . - 0 Rv0008c Rv0008c Unknown Possible membrane protein Rv0008c, (MTCY10H4.07c), len: 145 aa. Possible membrane protein. P9WJF3 No cell wall and cell processes P9WJF3 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 12468 13016 . + 0 Rv0009 ppiA PPIases accelerate the folding of proteins [catalytic activity: cis-trans isomerization of proline imidic peptide bonds in oligopeptides]. Probable iron-regulated peptidyl-prolyl cis-trans isomerase A PpiA (PPIase A) (rotamase A) Rv0009, (MTCY10H4.08), len: 182 aa. Probable ppiA (alternate gene name: cfp22), iron-regulated peptidyl-prolyl cis-trans isomerase A. Belongs to the cyclophilin-type PPIase family. Alternative start codon has been suggested. P9WHW3 No information pathways 1W74 P65762 P9WHW3 GO:0003755,GO:0006457,GO:0005737 5.2.1.8 P9WHW3 NC_000962.3 Mycobrowser_v5 CDS 583375 583704 . - 0 Rv0492A Rv0492A Unknown Hypothetical protein Rv0492A, len: 109 aa. Hypothetical unknown protein. GC plot suggests CDS. Q6MX36 No conserved hypotheticals Q6MX36 NC_000962.3 Mycobrowser_v5 CDS 13133 13558 . - 0 Rv0010c Rv0010c Unknown Probable conserved membrane protein Rv0010c, (MTCY10H4.10c), len: 141 aa. Probable conserved membrane protein. Belongs to superfamily DUF2581, conserved in the Actinomycetales. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WMA3 No cell wall and cell processes P71580 P9WMA3 GO:0005886,GO:0016021 P9WMA3 NC_000962.3 Mycobrowser_v5 CDS 13714 13995 . - 0 Rv0011c Rv0011c Unknown Probable conserved transmembrane protein Rv0011c, (MTCY10H4.11c), len: 93 aa. Probable conserved transmembrane protein. Belongs to uncharacterized protein family UPF0233. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WP57 No cell wall and cell processes P67376 P9WP57 GO:0005886,GO:0016021 P9WP57 NC_000962.3 Mycobrowser_v5 CDS 14089 14877 . + 0 Rv0012 Rv0012 Unknown Probable conserved membrane protein Rv0012, (MTCY10H4.12), len: 262 aa. Probable conserved membrane protein. Belongs to superfamily DUF881. Contains probable N-terminal signal sequence. L0T243 No cell wall and cell processes P71582 L0T243 L0T243 NC_000962.3 Mycobrowser_v5 CDS 15590 17470 . - 0 Rv0014c pknB Involved in signal transduction (via phosphorylation). Thought to regulate cell division/differentiation. Can phosphorylate the peptide substrate myelin basic protein (MBP) [catalytic activity: ATP + a protein = ADP + a phosphoprotein]. Transmembrane serine/threonine-protein kinase B PknB (protein kinase B) (STPK B) Rv0014c, (MTCY10H4.14c), len: 626 aa. PknB, transmembrane serine/threonine-protein kinase (see citations below). Contains PS00107 Protein kinases ATP-binding region signature, and PS00108 Serine/Threonine protein kinases active-site signature. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases. Experimental studies show evidence of auto-phosphorylation on serine/threonine residues. PknB has been shown to be a substrate for PstP and its kinase activity is affected by PstP-mediated dephosphorylation. PknB and PstP (Rv0018c) may act as a functional pair in vivo to control mycobacterial cell growth. P9WI81 No regulatory proteins 1MRU,1O6Y,2FUM,2KUD,2KUE,2KUF,2KUI,3F61,3F69 P0A5S4 P9WI81 GO:0046872,GO:0008658,GO:0006468,GO:0004674,GO:0005524 2.7.11.1 P9WI81 NC_000962.3 Mycobrowser_v5 CDS 18759 20234 . - 0 Rv0016c pbpA Involved in peptidoglycan synthesis (at the final stages). Cell wall formation; PBPA is supposed to be responsible for the determination of the rod shape of the cell. It synthesizes cross-linked peptidoglycan from lipid intermediates. Probable penicillin-binding protein PbpA Rv0016c, (MTCY10H4.16c), len: 491 aa. Probable pbpA, penicillin-binding protein, similar to many. P9WKD1 No cell wall and cell processes 3LO7 P71586 P9WKD1 GO:0016021,GO:0008658,GO:0009252,GO:0005886,GO:0008360,GO:0016740,GO:0007047 P9WKD1 NC_000962.3 Mycobrowser_v5 CDS 21637 23181 . - 0 Rv0018c pstP Involved in regulation (using dephosphorylation of a specific phosphorylated substrate). Phosphoserine/threonine phosphatase PstP Rv0018c, (MTCY10H4.18c), len: 514 aa. PstP, phosphoserine/threonine phosphatase. Experimental studies have shown that PstP specifically dephosporylates model phospho-Ser/Thr substrates and it is likely that PknB (Rv0014c) and PstP may act as a functional pair in vivo to control mycobacterial cell growth (See Boitel et al., 2003). P9WHW5 No regulatory proteins 1TXO,2CM1 P71588 P9WHW5 GO:0046872,GO:0004721,GO:0005886,GO:0016021 3.1.3.16 P9WHW5 NC_000962.3 Mycobrowser_v5 CDS 39877 41202 . - 0 Rv0037c Rv0037c Unknown; possibly involved in transport of macrolide across the membrane. Probable conserved integral membrane protein Rv0037c, (MTCY10H4.37c), len: 441 aa. Probable conserved integral membrane protein, member of major facilitator superfamily (MFS) possibly involved in transport of macrolide. P9WJY1 No cell wall and cell processes P0A5C1 P9WJY1 GO:0005886,GO:0055085,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 375914 376585 . + 0 Rv0306 Rv0306 Function unknown; probably involved in cellular metabolism. Putative oxidoreductase Rv0306, (MTCY63.11), len: 223 aa. Putative oxidoreductase, highly similar to H83485|9947208|AAG04663.1|AE004557_4|AE004557 conserved hypothetical protein from Pseudomonas aeruginosa strain PAO1 (218 aa); and to other putative oxidoreductases e.g. middle part of CAB76073.1|AL157953 putative nitroreductase from Streptomyces coelicolor (1212 aa); Q52685|BLUB protein involved in cobalamin (vitamin B12) synthesis from Rhodobacter capsulatus (206 aa), FASTA scores: opt: 318, E(): 2e-15, (35.6% identity in 191 aa overlap). O07233 No intermediary metabolism and respiration O07233 O07233 GO:0016491,GO:0055114 1.-.-.- O07233 NC_000962.3 Mycobrowser_v5 CDS 27595 28365 . + 0 Rv0023 Rv0023 Possibly involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0023, (MTCY10H4.23), len: 256 aa. Possible transcriptional regulator. Contains probable helix-turn helix motif from aa 19 to 40 (Score 1615, +4.69 SD). P9WMI3 No regulatory proteins P67704 P9WMI3 GO:0043565,GO:0006350,GO:0045449 P9WMI3 NC_000962.3 Mycobrowser_v5 CDS 29245 29607 . + 0 Rv0025 Rv0025 Function unknown Conserved hypothetical protein Rv0025, (MTCY10H4.25), len: 120 aa. Conserved hypothetical protein, showing some similarity to other proteins from Mycobacterium tuberculosis e.g. Rv0739 (268 aa), FASTA score: (37.6% identity in 101 aa overlap), and Rv0026 FASTA score: (35.4% identity in 113 aa overlap); etc. P9WMA1 No conserved hypotheticals P71595 P9WMA1 NC_000962.3 Mycobrowser_v5 CDS 29722 31068 . + 0 Rv0026 Rv0026 Function unknown Conserved hypothetical protein Rv0026, (MTCY10H4.26), len: 448 aa. Conserved hypothetical protein, showing some similarity to other proteins from Mycobacterium tuberculosis: Rv0025 FASTA score: (35.4% identity in 113 aa overlap) and Rv0739 (268 aa), FASTA score: (32.4% identity in 142 aa overlap). P9WMB1 No conserved hypotheticals P71596 P9WMB1 P9WMB1 NC_000962.3 Mycobrowser_v5 CDS 31189 31506 . + 0 Rv0027 Rv0027 Unknown Conserved hypothetical protein Rv0027, (MTCY10H4.27), len: 105 aa. Conserved hypothetical unknown protein. P9WM99 No conserved hypotheticals P64667 P9WM99 NC_000962.3 Mycobrowser_v5 CDS 32057 33154 . + 0 Rv0029 Rv0029 Function unknown Conserved hypothetical protein Rv0029, (MTCY10H4.29), len: 365 aa. Conserved hypothetical protein, showing some similarity to other proteins from Mycobacterium tuberculosis e.g. C-terminal region of Rv2082; Rv3899c. P71599 No conserved hypotheticals P71599 P71599 NC_000962.3 Mycobrowser_v5 CDS 34295 36610 . + 0 Rv0032 bioF2 Could be involved in biotin biosynthesis (at the first step) [catalytic activity: 6-carboxyhexanoyl-CoA + L-alanine = 8-amino-7-oxononanoate + CoA + CO2]. Possible 8-amino-7-oxononanoate synthase BioF2 (AONS) (8-amino-7-ketopelargonate synthase) (7-keto-8-amino-pelargonic acid synthetase) (7-KAP synthetase) (L-alanine--pimelyl CoA ligase) Rv0032, (MTCY10H4.32), len: 771 aa. Probable bioF2, 8-amino-7-oxononanoate synthase, with its C-terminal similar to others. Contains PS00599 Aminotransferases class-II pyridoxal-phosphate attachment site. Belongs to class-II of pyridoxal-phosphate-dependent aminotransferases. P9WQ85 No intermediary metabolism and respiration P71602 P9WQ85 GO:0008415,GO:0009058,GO:0016874,GO:0030170,GO:0008483,GO:0008710 2.3.1.47 P9WQ85 NC_000962.3 Mycobrowser_v5 CDS 36607 36870 . + 0 Rv0033 acpA Key component in de novo fatty acid biosynthesis. This protein is supposed to be the carrier of the growing fatty acid chain in fatty acid biosynthesis. Probable acyl carrier protein AcpA (ACP) Rv0033, (MTCY10H4.33), len: 87 aa. Probable acpA (alternate gene name: acpP), acyl carrier protein, similar to others. Also similar to proteins of Mycobacterium tuberculosis Rv1344 and Rv2244 (31.5% identity in 73 aa overlap). I6WX95 No lipid metabolism 2CGQ P71603 I6WX95 GO:0048037,GO:0000036 I6WX95 NC_000962.3 Mycobrowser_v5 CDS 36867 37262 . + 0 Rv0034 Rv0034 Function unknown Conserved hypothetical protein Rv0034, (MTCY10H4.34), len: 131 aa. Conserved hypothetical protein, showing weak similarity to AE001980|AE001980_7 hypothetical protein from Deinococcus radiodurans (120 aa), FASTA scores: opt: 141, E(): 0.0028, (29.3% identity in 123 aa overlap). P9WM93 No conserved hypotheticals P9WM93 P9WM93 NC_000962.3 Mycobrowser_v5 CDS 41304 41912 . + 0 Rv0038 Rv0038 Function unknown Conserved protein Rv0038, (MTCY10H4.38), len: 202 aa. Conserved protein, similar to many. P9WFK5 No conserved hypotheticals P67757 P9WFK5 P9WFK5 NC_000962.3 Mycobrowser_v5 CDS 42004 42351 . - 0 Rv0039c Rv0039c Unknown Possible conserved transmembrane protein Rv0039c, (MTCY21D4.02c, MTCY10H4.39c), len: 115 aa. Possible conserved transmembrane protein. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WM89 No cell wall and cell processes P9WM89 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 43562 46471 . + 0 Rv0041 leuS Involved in translation mechanism [catalytic activity: ATP + L-leucine + tRNA(LEU) = AMP + diphosphate + L-leucyl-tRNA(LEU)]. Probable leucyl-tRNA synthetase LeuS (leucine--tRNA ligase) (LEURS) Rv0041, (MTCY21D4.04), len: 969 aa. Probable leucyl-tRNA synthetase, similar to many. Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Belongs to class-I aminoacyl-tRNA synthetase family. P9WFV1 No information pathways P67510 P9WFV1 GO:0005737,GO:0004823,GO:0006429,GO:0005524 6.1.1.4 P9WFV1 NC_000962.3 Mycobrowser_v5 CDS 51185 51727 . - 0 Rv0047c Rv0047c Function unknown Conserved protein Rv0047c, (MTCY21D4.10c), len: 180 aa. Conserved protein, similar to many. P71704 No conserved hypotheticals P71704 P71704 P71704 NC_000962.3 Mycobrowser_v5 CDS 418949 419608 . + 0 Rv0349 Rv0349 Unknown Hypothetical protein Rv0349, (MTCY13E10.09), len: 219 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06300 No conserved hypotheticals O06300 NC_000962.3 Mycobrowser_v5 CDS 52831 53244 . + 0 Rv0049 Rv0049 Function unknown Conserved hypothetical protein Rv0049, (MTCY21D4.12), len: 137 aa. Conserved hypothetical protein. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WM85 No conserved hypotheticals P9WM85 NC_000962.3 Mycobrowser_v5 CDS 53663 55699 . + 0 Rv0050 ponA1 Involved in peptidoglycan synthesis (at the final stages), cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits). Probable bifunctional penicillin-binding protein 1A/1B PonA1 (murein polymerase) (PBP1): penicillin-insensitive transglycosylase (peptidoglycan TGASE) + penicillin-sensitive transpeptidase (DD-transpeptidase) Rv0050, (MTCY21D4.13), len: 678 aa. Probable ponA1, penicillin-binding protein (class A), bienzymatic protein with transglycosylase and transpeptidase activities (see Graham & Clark-Curtiss 1999), highly similar to many (see Billman-Jacobe et al., 1999). Belongs to the transglycosylase family in the N-terminal section, and to the transpeptidase family in the C-terminal section. P71707 No cell wall and cell processes P71707 P71707 GO:0016787,GO:0008658,GO:0009252,GO:0009274,GO:0008360,GO:0016757,GO:0007047 3.4.-.-,2.4.2.- P71707 NC_000962.3 Mycobrowser_v5 CDS 58192 58482 . + 0 Rv0053 rpsF Binds together with S18 to 16S ribosomal RNA. 30S ribosomal protein S6 RpsF Rv0053, (MTCY21D4.16), len: 96 aa. rpsF, 30S ribosomal protein S6, highly similar to many. Contains PS01048 Ribosomal protein S6 signature. Belongs to the S6P family of ribosomal proteins. P9WH31 No information pathways P66591 P9WH31 GO:0005840,GO:0003735,GO:0006412,GO:0019843 P9WH31 NC_000962.3 Mycobrowser_v5 CDS 63909 64967 . + 0 Rv0060 Rv0060 Function unknown Conserved hypothetical protein Rv0060, (MTV030.03), len: 352 aa. Conserved hypothetical protein. O53605 No conserved hypotheticals O53605 O53605 O53605 NC_000962.3 Mycobrowser_v5 CDS 65012 65350 . - 0 Rv0061c Rv0061c Unknown Hypothetical protein Rv0061c, len: 112 aa. Conserved hypothetical protein supported by RNA-seq data. Similar to MMAR_3839, 76% identity in 112 aa overlap. Replaces questionable ORF Rv0061 (MTV030.04). I6X8E6 No conserved hypotheticals I6X8E6 NC_000962.3 Mycobrowser_v5 CDS 65552 66694 . + 0 Rv0062 celA1 The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) exocellobiohydrolases that cut the dissaccharide cellobiose from the nonreducing end of the cellulose polymer chain; (3) beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose [catalytic activity:endohydrolysis of 1,4-beta-D-glucosidic linkages in cellulose]. Possible cellulase CelA1 (endoglucanase) (endo-1,4-beta-glucanase) (FI-cmcase) (carboxymethyl cellulase) Rv0062, (MTV030.05), len: 380 aa. Possible celA1, cellulase, similar to many. Seems to belong to cellulase family B (family 6 of glycosyl hydrolases). Note that previously known as celA. Q79G13 No intermediary metabolism and respiration 1UOZ,1UP0,1UP2,1UP3 Q79G13 Q79G13 GO:0030245,GO:0008810 3.2.1.4 Q79G13 NC_000962.3 Mycobrowser_v5 CDS 72274 74511 . - 0 Rv0066c icd2 Involved in the KREBS cycle [catalytic activity: isocitrate + NADP+ = 2-oxoglutarate + CO(2) + NADPH]. Probable isocitrate dehydrogenase [NADP] Icd2 (oxalosuccinate decarboxylase) (IDH) (NADP+-specific ICDH) (IDP) Rv0066c, (MTV030.09c), len: 745 aa. Probable icd2, isocitrate dehydrogenase NADP-dependent. Belongs to the monomeric-type family of IDH. Note that in H37Rv, Rv0066c is named icd2 and Rv3339c is icd1 while in CDC1551 and Erdman strains, Rv0066c is icd1 and Rv3339c is icd2. O53611 No intermediary metabolism and respiration O53611 O53611 GO:0046872,GO:0055114,GO:0006099,GO:0004450 1.1.1.42 O53611 NC_000962.3 Mycobrowser_v5 CDS 75301 76212 . + 0 Rv0068 Rv0068 Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv0068, (MTV030.11), len: 303 aa. Probable oxidoreductase, similar to many. O53613 No intermediary metabolism and respiration O53613 O53613 GO:0055114,GO:0016491 1.-.-.- O53613 NC_000962.3 Mycobrowser_v5 CDS 76237 77622 . - 0 Rv0069c sdaA Involved in gluconeogenesis from serine [catalytic activity: L-serine + H2O = pyruvate + NH3 + H2O]. Probable L-serine dehydratase SdaA (L-serine deaminase) (SDH) (L-SD) Rv0069c, (MTV030.12c), len: 461 aa. Probable sdaA, L-serine dehydratase. Belongs to the iron-sulfur dependent L-serine dehydratase family. Cofactor: iron-sulfur (4FE-4S) (probable). P9WGT5 No intermediary metabolism and respiration P66773 P9WGT5 GO:0003941,GO:0006094,GO:0046872,GO:0051539 4.3.1.17 P9WGT5 NC_000962.3 Mycobrowser_v5 CDS 81676 82668 . + 0 Rv0073 Rv0073 Thought to be involved in active transport of glutamine across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable glutamine-transport ATP-binding protein ABC transporter Rv0073, (MTV030.17), len: 330 aa. Probable glutamine-transport ATP-binding protein ABC-transporter (see citation below), similar to many ATP-binding proteins. Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00211 ABC transporters family signature, and PS00889 Cyclic nucleotide-binding domain signature 2. Belongs to the ATP-binding transport protein family (ABC transporters). Note that supposed act with near ORF Rv0072|MTV030.16 transmembrane ABC-transporter. P9WQK5 No cell wall and cell processes O53618 P9WQK5 GO:0016887,GO:0005952,GO:0008603,GO:0001932,GO:0005524 P9WQK5 NC_000962.3 Mycobrowser_v5 CDS 85636 86466 . - 0 Rv0077c Rv0077c Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv0077c, (MTV030.21c), len: 276 aa. Possible oxidoreductase, weakly similar to others from Streptomyces. Also similar to MTCY05A6_35 and MTCY1A11_10 from Mycobacterium tuberculosis. And shows some similarity in part with AAL17935.1|AY054120 putative epoxide hydrolase from Mycobacterium smegmatis (203 aa). O53622 No intermediary metabolism and respiration O53622 O53622 GO:0016491,GO:0055114 1.-.-.- O53622 NC_000962.3 Mycobrowser_v5 CDS 87208 87801 . - 0 Rv0078A Rv0078A Unknown Hypothetical protein Rv0078A, len: 197 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L7N686 No conserved hypotheticals Q8VKS0 L7N686 L7N686 NC_000962.3 Mycobrowser_v5 CDS 87798 88004 . - 0 Rv0078B Rv0078B Unknown Conserved protein Rv0078B, len: 68 aa. Conserved protein. I6X8G2 No conserved hypotheticals I6X8G2 I6X8G2 NC_000962.3 Mycobrowser_v5 CDS 88204 89025 . + 0 Rv0079 Rv0079 Unknown Unknown protein Rv0079, (MTV030.23), len: 273 aa. Unknown protein. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WMA9 No conserved hypotheticals P9WMA9 P9WMA9 NC_000962.3 Mycobrowser_v5 CDS 89022 89480 . + 0 Rv0080 Rv0080 Function unknown Conserved hypothetical protein Rv0080, (MTV030.24), len: 152 aa. Conserved hypothetical protein. Belongs to pyridoxine 5'-phosphate (PNP) oxidase-like (PNPOx-like) superfamily. P9WMA5 No conserved hypotheticals P9WMA5 P9WMA5 NC_000962.3 Mycobrowser_v5 CDS 90400 92322 . + 0 Rv0083 Rv0083 Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv0083, (MTV030.27, MTCY251.01), len: 640 aa. Probable oxidoreductase, showing some similarity to other various oxidoreductases. Nucleotide position 91071 in the genome sequence has been corrected, T:C resulting in I224I. P9WIW3 No intermediary metabolism and respiration Q10880 P9WIW3 GO:0008137,GO:0016021,GO:0005886,GO:0042773 NC_000962.3 Mycobrowser_v5 CDS 1211560 1213863 . + 0 Rv1087 PE_PGRS21 Function unknown PE-PGRS family protein PE_PGRS21 Rv1087, (MTV017.40), len: 767 aa. PE_PGRS21, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below). Similar to Rv1090|AL021897|MTV017_43 Mycobacterium tuberculosis H37Rv (853 aa), FASTA scores: opt: 2819, E(): 0, (59.8% identity in 860 aa overlap). Contains PS00583 pfkB family of carbohydrate kinases signature 1 near C -terminus. Predicted to be an outer membrane protein (See Song et al., 2008). Q79FT0 No PE/PPE Q79FT0 Q79FT0 Q79FT0 NC_000962.3 Mycobrowser_v5 CDS 98480 99250 . + 0 Rv0090 Rv0090 Unknown Possible membrane protein Rv0090, (MTCY251.08), len: 256 aa. Possible membrane protein. Contains IPR014511 Protein of unknown function DUF2068, transmembrane, subgroup. P9WM71 No cell wall and cell processes P64683 P9WM71 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 99684 100451 . + 0 Rv0091 mtn Responsible for cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH) [catalytic activity 1: methylthioadenosine + H2O = adenine + 5-methylthio-D-ribose] [catalytic activity 2: S-adenosyl-L-homocysteine + H2O = adenine + S-D-ribosyl-L-homocysteine]. Probable bifunctional MTA/SAH nucleosidase Mtn: 5'-methylthioadenosine nucleosidase (methylthioadenosine methylthioribohydrolase) + S-adenosylhomocysteine nucleosidase (S-adenosyl-L-homocysteine homocysteinylribohydrolase) Rv0091, (MTCY251.10), len: 255 aa. Probable mtn (alternate gene name: pfs), methylthioadenosine/S-Adenosylhomocysteine nucleosidase (MTA/SAH nucleosidase), including 5'-methylthioadenosine nucleosidase and S-adenosylhomocysteine nucleosidase, similar to others. Belongs to the MTN family. P9WJM3 No intermediary metabolism and respiration P67656 P9WJM3 GO:0008782,GO:0008930,GO:0009164,GO:0019509 3.2.2.9 P9WJM3 NC_000962.3 Mycobrowser_v5 CDS 100583 102868 . + 0 Rv0092 ctpA Cation-transporting ATPase; possibly catalyzes the transport of a cation (possibly copper) with the hydrolyse of ATP [catalytic activity: ATP + H(2)O + cation(in) = ADP + phosphate + cation(out)]. Cation transporter P-type ATPase a CtpA Rv0092, (MTCY251.11), len: 761 aa. CtpA, cation-transporting P-type ATPase a (transmembrane protein), highly similar to many. Contains PS01047 Heavy-metal-associated domain, and PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases), subfamily IB. P9WPU1 No cell wall and cell processes Q10876 P9WPU1 GO:0006754,GO:0004008,GO:0016021,GO:0046872,GO:0030001,GO:0005886,GO:0005524 3.6.3.- P9WPU1 NC_000962.3 Mycobrowser_v5 CDS 105324 106715 . + 0 Rv0096 PPE1 Function unknown PPE family protein PPE1 Rv0096, (MTCY251.15), len: 463 aa. PPE1, Member of the Mycobacterium tuberculosis PPE family, similar to many. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WI49 No PE/PPE Q10892 P9WI49 GO:0005886,GO:0016021 P9WI49 NC_000962.3 Mycobrowser_v5 CDS 108156 109778 . + 0 Rv0099 fadD10 Function unknown, but involvement in lipid degradation. Possible fatty-acid-CoA ligase FadD10 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv0099, (MTCY251.18), len: 540 aa. Possible fadD10, fatty-acid-CoA synthetase, similar to many. Contains PS00455 putative AMP-binding domain signature. Contains IPR000873 AMP-dependent synthetase/ligase domain. Belongs to the ATP-dependent AMP-binding enzyme family. P9WQ55 No lipid metabolism Q10878 P9WQ55 GO:0008152,GO:0016874 6.2.1.- P9WQ55 NC_000962.3 Mycobrowser_v5 CDS 110001 117539 . + 0 Rv0101 nrp Involved in lipid metabolism. Probable peptide synthetase Nrp (peptide synthase) Rv0101, (MTCY251.20), len: 2512 aa. Probable nrp, peptide synthetase, similar to others e.g. AAD44234.1|AF143772_40|PstB peptide synthetase from Mycobacterium avium (2552 aa); 7476034|S77657 cyclic peptide synthetase from Mycobacterium leprae (1401 aa), FASTA scores: opt: 4268, E(): 0, (65.7% identity in 1091 aa overlap); part of CAB55600.1|AJ238027 peptide synthetase from Mycobacterium smegmatis (5990). Also similar to e.g. AAD56240.1|AF184977_1|AF184977 DhbF protein from Bacillus subtilis (2378 aa); SRF1_BACSU|P27206 surfactin synthetase subunit 1 (3587 aa), FASTA scores: opt: 1708, E(): 0, (30.6% identity in 1633 aa overlap): etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), 2 x PS00455 Putative AMP-binding domain signature, and PS00012 Phosphopantetheine attachment site. Belongs to the ATP-dependent AMP-binding enzyme family. Thought to be not involved in mycobactin biosynthesis (see citation below). Q10896 No lipid metabolism Q10896 Q10896 GO:0000036,GO:0048037,GO:0016874,GO:0008152,GO:0031177,GO:0004043 6.-.-.- Q10896 NC_000962.3 Mycobrowser_v5 CDS 117714 119699 . + 0 Rv0102 Rv0102 Unknown Probable conserved integral membrane protein Rv0102, (MTCY251.21), len: 661 aa. Probable conserved integral membrane protein, highly similar to P53525|Y102_MYCLE|ML1998|NP_302349.1|NC_002677 possible membrane protein from Mycobacterium leprae (659 aa), FASTA scores: opt: 3107, E(): 0, (70.2% identity in 662 aa overlap). Also similar to others e.g. CAC01497.1|AL391017 putative integral membrane protein from Streptomyces coelicolor (316 aa); etc. Contains PS00343 Gram-positive cocci surface proteins 'anchoring' hexapeptide. P9WM63 No cell wall and cell processes P64689 P9WM63 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 124374 125570 . + 0 Rv0106 Rv0106 Function unknown Conserved hypothetical protein Rv0106, (MTCY251.25), len: 398 aa. Conserved hypothetical protein, similar to others e.g. AL049841|SCE9_33 from Streptomyces coelicolor (370 aa), FASTA scores: opt: 282, E(): 2.5e-11, (32.0% identity in 381 aa overlap); etc. Some similarity to P94400 homologue to nitrile hydratase region from Bacillus subtilis (397 aa), FASTA scores: opt: 226, E(): 5.4e-08, (26.4% identity in 405 aa overlap). Also similar to COBW_PSEDE|P29937 FASTA score: (25.3% identity in 186 aa overlap); and P47K_PSECL|P31521 47 kDa protein (p47k) (419 aa), FASTA score: (25.9% identity in 401 aa overlap). P9WPI5 No conserved hypotheticals P64691 P9WPI5 P9WPI5 NC_000962.3 Mycobrowser_v5 CDS 131382 132872 . + 0 Rv0109 PE_PGRS1 Function unknown PE-PGRS family protein PE_PGRS1 Rv0109, (MTV031.03c), len: 496 aa. PE_PGRS1, Member of the M. tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), highly similar to many e.g. Q50615|Y0DP_MYCTU hypothetical glycine-rich 40.8 kDa protein from Mycobacterium tuberculosis (498 aa), FASTA scores: opt: 1772, E(): 0, (57.3% identity in 513 aa overlap); etc. L0T2H7 No PE/PPE Q79G09 L0T2H7 L0T2H7 NC_000962.3 Mycobrowser_v5 CDS 137319 137909 . + 0 Rv0113 gmhA Involved in biosynthesis of nucleotide-activated glycero-manno-heptose: synthesis of glyceromannoheptose 7-phosphate (inner core lipopolysaccharide biosynthesis) [catalytic activity: D-sedoheptulose 7-phosphate = D-glycero-alpha,beta-D-manno-heptose 7-phosphate]. Probable sedoheptulose-7-phosphate isomerase GmhA (phosphoheptose isomerase) Rv0113, (MTV031.07), len: 196 aa. Probable gmhA (alternate gene name: lpcA), sedoheptulose-7-phosphate isomerase (see citation below), similar to many e.g. AE0005|HPAE000596_11 from Helicobacter pylori (192 aa), FASTA scores: opt: 451, E(): 1.9e-24, (45.1% identity in 162 aa overlap). Belongs to the sis family, LPCA subfamily. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WGG1 No cell wall and cell processes P0A604 P9WGG1 GO:0005975,GO:0005737,GO:0005529,GO:0008968 5.3.1.- P9WGG1 NC_000962.3 Mycobrowser_v5 CDS 137941 138513 . + 0 Rv0114 gmhB Involved in biosynthesis of nucleotide-activated glycero-manno-heptose. Involved in two pathways, D-alpha-D pathway [catalytic activity: D-glycero-alpha-D-manno-heptose 1,7-biphosphate = D-glycero-alpha-D-manno-heptose 1-phosphate] and L-beta-D pathway [catalytic activity: D-glycero-beta-D-manno-heptose 1,7-biphosphate = D-glycero-beta-D-manno-heptose 1-phosphate]. Possible D-alpha,beta-D-heptose-1,7-biphosphate phosphatase GmhB (D-glycero-D-manno-heptose 7-phosphate kinase) Rv0114, (MTV031.08), len: 190 aa. Possible gmhB, D-alpha,beta-D-heptose-1,7-biphosphate phosphatase (see citation below), similar to several hypothetical proteins and phosphatases e.g. HIS7_ECOLI|P06987 imidazoleglycerol-phosphate dehydratase (355 aa), FASTA scores: opt: 250, E(): 3.6e-11, (34.0 % identity in 141 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WMV3 No cell wall and cell processes P9WMV3 GO:0005737,GO:0000105,GO:0004401,GO:0005975 3.1.3.- P9WMV3 NC_000962.3 Mycobrowser_v5 CDS 142128 143876 . - 0 Rv0118c oxcA Involved in catabolism of oxalic acid [catalytic activity: oxalyl-CoA = formyl-CoA + CO2]. Probable oxalyl-CoA decarboxylase OxcA Rv0118c, (MTV031.12c), Len: 582 aa. Probable oxcA, oxalyl-CoA decarboxylase, highly similar to many e.g. P78093|OXC_ECOLI|7449483|B65011|YFDU|B2373|Z3637|ECS325 probable oxalyl-CoA decarboxylase from Escherichia coli (564 aa); M77128|OXAOXA_1 oxalyl-CoA decarboxylase from Oxalobacter formigenes (568 aa), FASTA scores: opt: 2124, E():0, (55.6% identity in 568 aa overlap). Also similar to mycobacterial IlvB proteins e.g. MLCB1788.46c unknown TPP-requiring enzyme from Mycobacterium leprae (548 aa); and AL0086|MLCB1788_19 from Mycobacterium leprae (548 aa), FASTA scores: opt: 831, E(): 0, (33.9% identity in 567 aa overlap). O53639 No intermediary metabolism and respiration O53639 O53639 GO:0033611,GO:0008949,GO:0030976,GO:0000287 4.1.1.8 O53639 NC_000962.3 Mycobrowser_v5 CDS 151148 152215 . + 0 Rv0125 pepA Function unknown; possibly hydrolyzes peptides and/or proteins (seems to cleave preferentially after serine residues). Probable serine protease PepA (serine proteinase) (MTB32A) Rv0125, (MTCI418B.07, MTB32A), len: 355 aa. Probable pepA (alternate gene name: mtb32a), serine protease (see Skeiky et al., 1999), highly similar to other proteases e.g. HHOB_ECOLI|P31137 protease hhob precursor (355 aa), FASTA scores: opt: 400, E(): 3.8e-14, (32.4% identity in 346 aa overlap). Also similar to Q50320 34 kDa protein precursor from Mycobacterium tuberculosis (361 aa), FASTA scores: opt: 1689, E(): 0, (70.7% identity in 362 aa overlap). Contains PS00135 Serine proteases, trypsin family, serine active site. Has a putative signal sequence at the N-terminus. Belongs to the serine protease family. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). O07175 No intermediary metabolism and respiration O07175 O07175 GO:0006508,GO:0004252,GO:0005515 3.4.21.- O07175 NC_000962.3 Mycobrowser_v5 CDS 152324 154129 . + 0 Rv0126 treS Involved in trehalose biosynthesis (protective effect). Converts maltose to trehalose. Mycobacteria can produce trehalose from glucose 6-phosphate and UDP-glucose (the OtsA-OtsB pathway) from glycogen-like alpha(1-->4)-linked glucose polymers (the TreY-TreZ pathway) and from maltose (the TreS pathway). Trehalose synthase TreS Rv0126, (MTCI418B.08), len: 601 aa. TreS, trehalose synthase (see citation below), highly similar to others e.g. CAA04601.2|AJ001205 putative trehalose synthase from Streptomyces coelicolor (566 aa); S71450|1536814|BAA11303.1|D78198 trehalose synthase maltose-specific from Pimelobacter sp. strain R48 (573 aa). Also similar to MAL1_DROME|P07191 possible maltase precursor (508 aa), FASTA scores: opt: 807, E(): 0, (33.7% identity in 504 aa overlap); and similar to proteins associated with amino-acid transport e.g. Q64319 rat protein which stimulates transport of cystine and dibasic and neutral amino acids (683 aa), FASTA scores: opt: 839, E(): 0, (32.0% identity in 531 aa overlap). Also similar to several other Mycobacterium tuberculosis proteins e.g. Rv2471 FASTA score: (31.7% identity in 164 aa overlap). P9WQ19 No virulence, detoxification, adaptation O07176 P9WQ19 GO:0043169,GO:0016853,GO:0005975 5.4.99.- P9WQ19 NC_000962.3 Mycobrowser_v5 CDS 166910 167290 . + 0 Rv0140 Rv0140 Function unknown Conserved protein Rv0140, (MTCI5.14), len: 126 aa. Conserved protein, similar to others e.g. P74567|D90916_48 hypothetical 20.8 KDP protein from Synechocystis sp. (180 aa), FASTA scores: opt: 229, E(): 4.7e-10, (36.1% identity in 108 aa overlap). Also similar to Rv1056 and Rv1670 from Mycobacterium tuberculosis. P96817 No conserved hypotheticals P96817 P96817 P96817 NC_000962.3 Mycobrowser_v5 CDS 156578 157600 . - 0 Rv0129c fbpC Proteins of the antigen 85 complex are responsible for the high affinity of mycobacteria to fibronectin. Possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity. Secreted antigen 85-C FbpC (85C) (antigen 85 complex C) (AG58C) (mycolyl transferase 85C) (fibronectin-binding protein C) Rv0129c, (MT0137, MTCI5.03c), len: 340 aa. FbpC (alternate gene names: mpt45, 85C, fbpC2), secreted antigen 85c (fibronectin-binding protein C) (mycolyl transferase 85C) (see citations below), also highly similar to other Mycobacterial antigen precursors e.g. A85C_MYCLE|Q05862 antigen 85-c precursor (85c) from Mycobacterium leprae (333 aa), FASTA scores: opt: 1937, E(): 0, (81.4% identity in 333 aa overlap); etc. P9WQN9 No lipid metabolism 1DQY,1DQZ,1VA5,3HRH P0A4V4 P9WQN9 GO:0005576,GO:0008415 2.3.1.- INH P9WQN9 NC_000962.3 Mycobrowser_v5 CDS 162644 163249 . - 0 Rv0135c Rv0135c Could be involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0135c, (MTCI5.09c), len: 201 aa. Possible transcriptional regulator, weakly similar to others e.g. P32398|YHGD_BACSU hypothetical transcriptional regulator from Bacillus subtilis (191 aa), FASTA scores: opt: 145, E(): 0.0012, (21.0% identity in 162 aa overlap). P96812 No regulatory proteins P96812 P96812 GO:0006350,GO:0003700,GO:0006355 P96812 NC_000962.3 Mycobrowser_v5 CDS 163366 164691 . + 0 Rv0136 cyp138 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Probable cytochrome P450 138 Cyp138 Rv0136, (MT0144, MTCI5.10), len: 441 aa. Probable cyp138, cytochrome P450 138, similar to others e.g. SLR0574|Q59990 from synechocystis SP. (444 aa), FASTA scores: opt: 315, E(): 1e-13, (25.7% identity in 416 aa overlap); etc. Also similar to MTV039_6 from Mycobacterium tuberculosis (472 aa), FASTA score: (38.2% identity in 442 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop); and PS00086 Cytochrome P450 cysteine heme-iron ligand signature. Belongs to the cytochrome P450 family. P9WPM3 No intermediary metabolism and respiration P63717 P9WPM3 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPM3 NC_000962.3 Mycobrowser_v5 CDS 164712 165260 . - 0 Rv0137c msrA Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine [catalytic activity: protein L-methionine + oxidized thioredoxin + H2O = protein-L-methionine-(S)-S-oxide + reduced thioredoxin]. Probable peptide methionine sulfoxide reductase MsrA (protein-methionine-S-oxide reductase) (peptide met(O) reductase) Rv0137c, (MTCI5.11c), len: 182 aa. Probable msrA, peptide methionine sulfoxide reductase (See St. John et al., 2001), equivalent to CAC32179.1|AL583926 putative peptide methionine sulfoxide from Mycobacterium leprae (177 aa). Highly similar to others e.g. CAC18703.1|AL451182 putative peptide methionine sulfoxide reductase from Streptomyces coelicolor (172 aa); PMSR_SCHPO|Q09859 putative peptide methionine sulfoxide reductase from Streptomyces (187 aa), FASTA scores: opt: 468, E(): 9.9e-26, (45.6% identity in 158 aa overlap); etc. Belongs to the MsrA family. P9WJM5 No intermediary metabolism and respiration 1NWA P0A5L0 P9WJM5 GO:0008113,GO:0006464,GO:0055114 1.8.4.11 P9WJM5 NC_000962.3 Mycobrowser_v5 CDS 167711 168637 . + 0 Rv0142 Rv0142 Function unknown Conserved hypothetical protein Rv0142, (MTCI5.16), len: 308 aa. Conserved hypothetical protein, similar, except in N-terminus, to AB88922.1|AL353862 hypothetical protein SCE34.20 from Streptomyces coelicolor (326 aa). P96819 No conserved hypotheticals P96819 GO:0003824,GO:0006281 P96819 NC_000962.3 Mycobrowser_v5 CDS 168704 170182 . - 0 Rv0143c Rv0143c Unknown; possibly ion channel involved in transport of chloride across the membrane. Probable conserved transmembrane protein Rv0143c, (MTCI5.17c), len: 492 aa. Probable conserved transmembrane protein, CIC family possibly involved in transport of chloride, similar to others and hypothetical proteins e.g. O28857 putative chloride channel from Archaeoglobus fulgidus (589 aa), FASTA scores: opt: 966, E(): 0, (37.7% identity in 453 aa overlap); YADQ_ECOLI|P37019 hypothetical 46.0 kDa protein (436 aa), FASTA scores: opt: 452, E(): 2.4e-20, (28.0% identity in 460 aa overlap). P96820 No cell wall and cell processes P96820 P96820 GO:0016021,GO:0055085,GO:0005247,GO:0006821 P96820 NC_000962.3 Mycobrowser_v5 CDS 173238 174758 . + 0 Rv0147 Rv0147 Function unknown; probably involved in cellular metabolism [catalytic activity: an aldehyde + NAD+ + H2O = an acid + NADH]. Probable aldehyde dehydrogenase (NAD+) dependent Rv0147, (MTCI5.21), len: 506 aa. Probable aldehyde dehydrogenase (NAD+) dependent, similar to others e.g. DHAP_RAT|P11883 aldehyde dehydrogenase (dimeric NADP-preferring) (452 aa), FASTA scores: opt: 1291, E(): 0, (43.9% identity in 453 aa overlap). Also similar to several Mycobacterium tuberculosis aldehyde dehydrogenases e.g. Rv0768, Rv2858c, etc. Contains PS00687 aldehyde dehydrogenases glutamic acid active site, and PS00070 aldehyde dehydrogenases cysteine active site. Belongs to the aldehyde dehydrogenases family. P96824 No intermediary metabolism and respiration P96824 P96824 GO:0006081,GO:0055114,GO:0004030 1.2.1.- P96824 NC_000962.3 Mycobrowser_v5 CDS 177543 179309 . - 0 Rv0151c PE1 Function unknown PE family protein PE1 Rv0151c, (MTCI5.25c), len: 588 aa. PE1, Member of the Mycobacterium tuberculosis PE family (see citation below), with N-terminal region similar to others e.g. MTV032_2 PE_PGRS family from Mycobacterium tuberculosis (468 aa), FASTA scores: opt: 1125, E(): 0, (46.3% identity in 456 aa overlap); MTCY493_24 from Mycobacterium tuberculosis FASTA score: (42.5% identity in 558 aa overlap). Also similar to upstream ORF MTCI5.26c FASTA score: (54.7% identity in 464 aa overlap). Also shows similarity to C-terminal part of some PPE family proteins e.g. MTV049_21 from Mycobacterium tuberculosis FASTA score: (41.5% identity in 591 aa overlap). Q79G06 No PE/PPE Q79G06 Q79G06 Q79G06 NC_000962.3 Mycobrowser_v5 CDS 179319 180896 . - 0 Rv0152c PE2 Function unknown PE family protein PE2 Rv0152c, (MTCI5.26c), len: 525 aa. PE2, Member of the Mycobacterium tuberculosis PE family (see citation below), similar to ORF downstream Z92770|MTCI5_25 (588 aa), FASTA scores: opt: 1492, E(): 0, (54.7% identity in 464 aa overlap); and to many other PE family type members. Predicted to be an outer membrane protein (See Song et al., 2008). Q79G05 No PE/PPE Q79G05 Q79G05 Q79G05 NC_000962.3 Mycobrowser_v5 CDS 181155 181985 . - 0 Rv0153c ptbB Involved in signal transduction (via dephosphorylation). Can dephosphorylate in vitro the phosphotyrosine residue of myelin basic protein (MBP) at pH 7.0. Could be involved in virulence by interfering with phosphotyrosine-mediated signals in macrophages. Also able to dephosphorylate phosphoserine/threonine peptides and phosphoinositide substrates. [catalytic activity: protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate] Phosphotyrosine protein phosphatase PTPB (protein-tyrosine-phosphatase) (PTPase) Rv0153c, (MTCI5.27c), len: 276 aa. PtbB (alternate gene name: MPtpB), protein-tyrosine-phosphatase (see citation below), showing some similarity to several protein-tyrosine phosphatases, polyketide synthase and aminotransferase e.g. Q05918|IPHP_NOSCO|IPH protein-tyrosine-phosphatase precursor from Nostoc commune (294 aa), FASTA scores: opt: 150, E(): 0.0096, (26.8% identity in 269 aa overlap); etc. Supposedly a secreted protein. Potent and selective inhibitor is an isoxazole compound (See Seollner et al., 2007). I6WXK4 No regulatory proteins 1YWF,2OZ5 I6WXK4 GO:0004725,GO:0016311 3.1.3.48 I6WXK4 NC_000962.3 Mycobrowser_v5 CDS 185052 186479 . + 0 Rv0157 pntB The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [catalytic activity: NADPH + NAD+ = NADP+ + NADH]. Probable NAD(P) transhydrogenase (subunit beta) PntB [integral membrane protein] (pyridine nucleotide transhydrogenase subunit beta) (nicotinamide nucleotide transhydrogenase subunit beta) Rv0157, (MTCI5.31), len: 475 aa. Probable pntB, pyridine nucleotide transhydrogenase (nicotinamide nucleotide transhydrogenase) subunit beta, integral membrane protein, similar to others e.g. Q59763 proton-translocating nicotinamide nucleotide transhydrogenase subunit beta from hodospirillum rubrum (464 aa), FASTA scores: opt: 1344, E(): 0, (46.4% identity in 472 aa overlap); P07002|PNTB_ECOLI|P76890|PNTB|B1602|Z2597|ECS2308 NAD(P) transhydrogenase subunit beta from Escherichia coli strains K12 and O157:H7 (462 aa). P96834 No intermediary metabolism and respiration P96834 P96834 GO:0050661,GO:0016021,GO:0055114,GO:0008750 1.6.1.2 P96834 NC_000962.3 Mycobrowser_v5 CDS 190607 191956 . + 0 Rv0161 Rv0161 Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0161, (MTCI28.01, MTV032.04), len: 449 aa. Possible oxidoreductase, similar to hypothetical proteins and various oxidoreductases e.g. AIP2_YEAST|P46681 actin interacting protein 2 (530 aa), FASTA scores: opt: 356, E (): 0, (33.3% identity in 357 aa overlap); DLD1_YEAST|P32891 d-lactate dehydrogenase (cytochrome) (587 aa), FASTA scores: opt: 311, E(): 2.5e-20, (27.9% identity in 366 aa overlap). Also similar to other Mycobacteria proteins e.g. MTCY339.30c from Mycobacterium tuberculosis FASTA score: (29.4% identity in 357 aa overlap); MLCL622.30c from Mycobacterium tuberculosis (449 aa). O07406 No intermediary metabolism and respiration O07406 O07406 GO:0055114,GO:0016491,GO:0050660 1.-.-.- O07406 NC_000962.3 Mycobrowser_v5 CDS 194993 196657 . + 0 Rv0166 fadD5 Function unknown, but involved in lipid degradation. Probable fatty-acid-CoA ligase FadD5 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv0166, (MTCI28.06), len: 554 aa. Probable fadD5, fatty-acid-CoA synthetase, similar to many eg LCFA_ECOLI|P29212 long-chain-fatty-acid--CoA ligase (561 aa), FASTA scores: opt: 612, E(): 0, (29.4% identity in 534 aa overlap). Also similar to many other fatty-acid-CoA ligases from Mycobacterium tuberculosis e.g. MTCY07A7.11c FASTA score: (35.3% identity in 487 aa overlap), MTV013_10, MTY25D10_30, etc. Contains PS00455 putative AMP-binding domain signature. O07411 No lipid metabolism O07411 O07411 GO:0008152,GO:0016874 6.2.1.- O07411 NC_000962.3 Mycobrowser_v5 CDS 198534 199898 . + 0 Rv0169 mce1A Unknown, but thought to be involved in host cell invasion (entry and survival inside macrophages). Mce-family protein Mce1A Rv0169, (MTCI28.09), len: 454 aa. Mce1A; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07789|MCE2|Rv0589|MTCY19H5.33c|mce2A (404 aa); O53967|MCE3|Rv1966|MTV051.04|mce3A (425 aa); etc. Also highly similar to others e.g. AAD52105.1|AF113402_1|AF113402 mycobacterial cell entry protein from Mycobacterium bovis BCG (454 aa); NP_302656.1|NC_002677 putative cell invasion protein from Mycobacterium leprae (441 aa); AAA92845.1|U26018 mce gene product from Mycobacterium avium (88 aa) (similarity on C-terminus); CAC12798.1|AL445327 putative secreted protein from Streptomyces coelicolor (418 aa); etc. Note that equivalent, but longer 22 aa, to P72013|CAA50257.1|X70901 Mcep protein from Mycobacterium tuberculosis (432 aa). Contains a very hydrophobic region around residues 20-35. Note that previously known as mce1. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). Q79FZ9 No virulence, detoxification, adaptation Q79FZ9 Q79FZ9 Q79FZ9 NC_000962.3 Mycobrowser_v5 CDS 200932 202479 . + 0 Rv0171 mce1C Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce1C Rv0171, (MTCI28.11), len: 515 aa. Mce1C; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07787|Rv0591|MTCY19H5.31|mce2C (481 aa); O53969|Rv1968|MTV051.06|mce3C (410 aa); etc. Also highly similar to others e.g. NP_302658.1|NC_002677 putative secreted protein from Mycobacterium leprae (519 aa); CAC12796.1|AL445327 putative secreted protein from Streptomyces coelicolor (351 aa); etc. Weakly similar to downstream ORF Rv0172|MTCI28.12|mce1D (530 aa), FASTA score: (24.6% identity in 552 aa overlap). Contains possible signal sequence and highly proline-rich C-terminus. Predicted to be an outer membrane protein (See Song et al., 2008). O07415 No virulence, detoxification, adaptation O07415 O07415 O07415 NC_000962.3 Mycobrowser_v5 CDS 205231 206778 . + 0 Rv0174 mce1F Unknown, but thought involved in host cell invasion. Mce-family protein Mce1F Rv0174, (MTCI28.14), len: 515 aa. Mce1F; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), similar to Mycobacterium tuberculosis proteins O07784|Rv0594|MTCY19H5.28c|mce2F (516 aa); O53972|Rv1971|MTV051.09|mce3F (437 aa); etc. Also highly similar to others e.g. NP_302661.1|NC_002677 putative secreted protein from Mycobacterium leprae (516 aa); AAF74993.1|AF143400_1|AF143400|996A027a protein from Mycobacterium avium (80 aa) (similarity on C-terminus); CAC12793.1|AL445327 putative secreted protein from Streptomyces coelicolor (433 aa); etc. Has hydrophobic stretch, possibly a signal peptide at the N-terminus. Predicted to be an outer membrane protein (See Song et al., 2008). L0T2W6 No virulence, detoxification, adaptation O07418 L0T2W6 L0T2W6 NC_000962.3 Mycobrowser_v5 CDS 208938 209672 . + 0 Rv0178 Rv0178 Unknown Probable conserved Mce associated membrane protein Rv0178, (MTCI28.18), len: 244 aa. Probable conserved Mce-associated membrane protein, highly similar in C-terminus to CAC32130.1|AL583926 putative secreted protein from Mycobacterium leprae (184 aa). Also similar to mce-associated proteins from Mycobacterium tuberculosis e.g. Rv1363c, Rv0177, Rv1973, etc. Note that there is a 10 aa overlap with the upstream ORF. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O07422 No cell wall and cell processes O07422 O07422 NC_000962.3 Mycobrowser_v5 CDS 215715 216224 . + 0 Rv0185 Rv0185 Function unknown; probably involved in a cellular metabolism. Conserved hypothetical protein Rv0185, (MTCI28.25a), len: 169 aa. Conserved hypothetical protein, equivalent to CAB08794.1|Z95398|MLCL622_2 from Mycobacterium leprae (168 aa), FASTA scores: opt: 861, E(): 0, (76.4% identity in 165 aa overlap). Contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O07429 No conserved hypotheticals O07429 NC_000962.3 Mycobrowser_v5 CDS 237206 237895 . + 0 Rv0200 Rv0200 Unknown Possible conserved transmembrane protein Rv0200, (MTV033.08), len: 229 aa. Possible conserved transmembrane protein, equivalent to Z95398|MLCL622.14 from Mycobacterium leprae (229 aa), FASTA scores: opt: 1147, E(): 0, (74.7% identity in 229 aa overlap). Also some similarity to Rv1973 from Mycobacterium tuberculosis (160 aa); and Rv1362c|Z75555|MTCY02B10_26 (220 aa), FASTA scores: opt: 134, E(): 0.063, (25.8% identity in 159 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53651 No cell wall and cell processes O53651 GO:0016021 O53651 NC_000962.3 Mycobrowser_v5 CDS 219996 221723 . - 0 Rv0189c ilvD Involved in valine and isoleucine biosynthesis (at the fourth step) [catalytic activity: 2,3-dihydroxy-3-methylbutanoate = 3-methyl-2- oxobutanoate + H(2)O]. Probable dihydroxy-acid dehydratase IlvD (dad) Rv0189c, (MTCI28.28c), len: 575 aa. Probable ilvD, dihydroxy-acid dehydratase, similar to many e.g. ILVD_LACLA|Q02139 dihydroxy-acid dehydratase (dad) from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (570 aa), FASTA scores: opt: 1605, E(): 0, (46.0% identity in 561 aa overlap). Also similar to ML2608|MLCL622.06c|O06069|ILVD_MYCLE dihydroxy-acid dehydratase from Mycobacterium leprae (564 aa). Contains PS00886 Dihydroxy-acid and 6-phosphogluconate dehydratases signature 1. Belongs to the ILVD / EDD family. Cofactor: binds 1 4FE-4S cluster (potential). P9WKJ5 No intermediary metabolism and respiration P65154 P9WKJ5 GO:0004160,GO:0009097,GO:0046872,GO:0009099,GO:0051539 4.2.1.9 P9WKJ5 NC_000962.3 Mycobrowser_v5 CDS 230899 231534 . + 0 Rv0195 Rv0195 Possibly sensor part of a two component regulatory system. Possible two component transcriptional regulatory protein (probably LuxR-family) Rv0195, (MTV033.03), len: 211 aa. Possible two-component response regulator, luxR family, similar to many e.g. U00008|ECOHU49_15 regulatory protein narP from Escherichia coli strain K12 (225 aa), FASTA scores: opt: 232, E(): 7.3e-09, (29.2% identity in 219 aa overlap). Start chosen by similarity. Contains probable helix-turn-helix motif at aa 166-187 (Score 1164, +3.15 SD). O53646 No regulatory proteins O53646 O53646 GO:0006355,GO:0043565,GO:0006350,GO:0003700,GO:0000156,GO:0000160,GO:0005622 O53646 NC_000962.3 Mycobrowser_v5 CDS 232231 234519 . + 0 Rv0197 Rv0197 Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0197, (MTV033.05), len: 762 aa. Possible oxidoreductase, similar to others e.g. 9948789|AAG06102.1|AE004699_7|B83307 probable molybdopterin oxidoreductase from Pseudomonas aeruginosa strain PAO1 (769 aa); 5441785|CAB46809.1|AL096811|T36812 probable dehydrogenase from Streptomyces coelicolor (747 aa), FASTA scores: opt: 617, E(): 9.8e-30, (29.9% identity in 762 aa overlap); BAB04334.1|AP001509 assimilatory nitrate reductase (catalytic subunit) from Bacillus halodurans (743 aa); etc. L0T2Z1 No intermediary metabolism and respiration O53648 L0T2Z1 GO:0030151,GO:0055114,GO:0016491,GO:0051536 1.-.-.- L0T2Z1 NC_000962.3 Mycobrowser_v5 CDS 454421 455386 . + 0 Rv0377 Rv0377 Possibly involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably LysR-family) Rv0377, (MTV036.12), len: 321 aa. Probable transcription regulator, lysR family, showing similarity with many hypothetical transcriptional regulators lysR homolog e.g. P32484|YEIE_ECOLI|M89774 hypothetical transcriptional regulator from Escherichia coli (293 aa), FASTA scores: opt: 265, E(): 4.9e-11, (28.6% identity in 266 aa overlap). Also similar to Rv2282c from Mycobacterium tuberculosis. Contains PS00044 bacterial regulatory protein lysR family signature. Seems to belong to the LysR family of transcriptional regulators. P9WMF7 No regulatory proteins O53712 P9WMF7 GO:0006350,GO:0003700,GO:0006355 P9WMF7 NC_000962.3 Mycobrowser_v5 CDS 238392 241292 . - 0 Rv0202c mmpL11 Unknown. Thought to be involved in fatty acid transport. Probable conserved transmembrane transport protein MmpL11 Rv0202c, (MTV033.10c), len: 966 aa. Probable mmpL11, conserved transmembrane transport protein (see citation below), equivalent to Z95398|MLCL622.16c from Mycobacterium leprae (1014 aa), FASTA scores: opt: 4076, E(): 0, (72.8% identity in 1017 aa overlap). Member of RND superfamily, similar to several putative transport proteins e.g. P96687 from Bacillus subtilis (724 aa), FASTA scores: opt: 594, E(): 9.1e-29, (26.9% identity in 717 aa overlap); etc. Belongs to the MmpL family. P9WJT9 No cell wall and cell processes P65374 P9WJT9 GO:0005886,GO:0016021 P9WJT9 NC_000962.3 Mycobrowser_v5 CDS 250120 251598 . + 0 Rv0210 Rv0210 Unknown Hypothetical protein Rv0210, (MTCY08D5.05), len: 492 aa. Hypothetical unknown protein. Possibly membrane protein; has hydrophobic stretches around aa 333 - 381. P96392 No conserved hypotheticals P96392 P96392 NC_000962.3 Mycobrowser_v5 CDS 251782 253602 . + 0 Rv0211 pckA Rate-limiting gluconeogenic enzyme [catalytic activity: GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2]. Probable iron-regulated phosphoenolpyruvate carboxykinase [GTP] PckA (phosphoenolpyruvate carboxylase) (PEPCK)(pep carboxykinase) Rv0211, (MTCY08D5.06), len: 606 aa. Probable pckA (alternate gene names: pckG and pck1), iron-regulated phosphoenolpyruvate carboxykinase [GTP], equivalent to Z95398|MLCL622_21 probable phosphoenolpyruvate carboxykinase from Mycobacterium leprae (609 aa), FASTA score: (86.1% identity in 605 aa overlap). Also highly similar to others e.g. PPCK_NEOFR|P22130 phosphoenolpyruvate carboxykinase [GTP] (608 aa), FASTA scores: opt: 2287, E(): 0, (55.9% identity in 598 aa overlap). Contains PS00505 Phosphoenolpyruvate carboxykinase (GTP) signature. Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. P9WIH3 No intermediary metabolism and respiration P65686 P9WIH3 GO:0005737,GO:0006094,GO:0046872,GO:0004613,GO:0005525 4.1.1.32 P9WIH3 NC_000962.3 Mycobrowser_v5 CDS 296005 296493 . + 0 Rv0245 Rv0245 Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv0245, (MTV034.11), len: 162 aa. Possible oxidoreductase, equivalent to AL022486|MLCB1883_17|T44882 probable oxidoreductase from Mycobacterium leprae (162 aa), FASTA scores: opt: 860, E(): 0, (83.4% identity in 157 aa overlap). Also similar to several hypothetical proteins and various oxidoreductases e.g. AAK24246.1|AE005898 NADH:riboflavin 5'-phosphate oxidoreductase from Caulobacter crescentus (174 aa); Q02058|DIM6_STRCO|CAA45048.1 actinorhodin polyketide dimerase from streptomyces coelicolor (177 aa), FASTA scores: opt: 308, E(): 3. 2e-15, (37.8% identity in 143 aa overlap). Also similar to Z84498|Rv1939|MTCY09F9.25c from Mycobacterium tuberculosis (171 aa), FASTA scores: opt: 517, E(): 3.5e-30, (49.4% identity in 158 aa overlap). O53667 No intermediary metabolism and respiration O53667 O53667 GO:0055114,GO:0016491,GO:0010181 1.-.-.- O53667 NC_000962.3 Mycobrowser_v5 CDS 256064 257677 . + 0 Rv0214 fadD4 Function unknown, but involved in lipid degradation. Probable fatty-acid-CoA ligase FadD4 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv0214, (MTCY08D5.09), len: 537 aa. Probable fadD4, fatty-acid-CoA synthetase, similar to many e.g. 4CL_PINTA|P41636 4-coumarate--CoA ligase (537 aa), FASTA scores: opt: 622, E(): 1e-31, (30.0% identity in 514 aa overlap). Also similar to others from Mycobacterium tuberculosis e.g. MTCY6A4.14 FASTA score: (30.7% identity in 501 aa overlap); MTCY493_27, MTCY07A7_11, MTCI28_6. Contains PS00455 putative AMP-binding domain signature. P96396 No lipid metabolism P96396 P96396 GO:0008152,GO:0016874 6.2.1.- P96396 NC_000962.3 Mycobrowser_v5 CDS 262812 264023 . + 0 Rv0220 lipC Function unknown; lipolytic enzyme probably involved in cellular metabolism. Probable esterase LipC Rv0220, (MTCY08D5.15), len: 403 aa. Probable esterase, similar to others proteins and esterases from various organisms and Mycobacterium tuberculosis e.g. Q50681 (431 aa), FASTA scores: opt: 841, E(): 0, (38.2% identity in 408 aa overlap); Rv1426c, Rv1399c, etc. Contains PS00122 Carboxylesterases type-B serine active site. P96402 No intermediary metabolism and respiration P96402 P96402 GO:0016787 3.1.1.- P96402 NC_000962.3 Mycobrowser_v5 CDS 266301 267764 . - 0 Rv0223c Rv0223c Thought to oxidize a wide variety of aliphatic and aromatic aldehydes. Probable aldehyde dehydrogenase Rv0223c, (MTCY08D5.18), len: 487 aa. Probable aldehyde dehydrogenase, similar to others e.g. A75608|6460525|AAF12231.1|AE001862_57 aldehyde dehydrogenase from Deinococcus radiodurans strain R1 (495 aa); Q47943 L-sorbosone dehydrogenase NAD(P) dependent from Gluconobacter oxydans (498 aa), FASTA scores: opt: 1157, E (): 0, (42.1% identity in 482 aa overlap); etc. Also similar to Rv0768, Rv2858c, etc from Mycobacterium tuberculosis. Contains PS00687 Aldehyde dehydrogenases glutamic acid active site; and PS00070 Aldehyde dehydrogenases cysteine active site. Belongs to the aldehyde dehydrogenases family. I6X8S7 No intermediary metabolism and respiration 3B4W P96405 I6X8S7 GO:0016491,GO:0055114 1.2.1.- I6X8S7 NC_000962.3 Mycobrowser_v5 CDS 269834 271564 . - 0 Rv0226c Rv0226c Unknown Probable conserved transmembrane protein Rv0226c, (MTCY08D5.21c), len: 576 aa. Probable conserved transmembrane protein, equivalent, except in N-terminal part, to AC32114.1|AL583926 conserved membrane protein from Mycobacterium leprae (600 aa), FASTA scores: opt: 2086, E(): 0, (70.3% identity in 579 aa overlap). Also similar to AL021411|SC7H1_20 from Streptomyces coelicolor (483 aa), FASTA scores: opt: 180, E(): 0.00028, (26.5 identity in 388 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P96408 No cell wall and cell processes P96408 GO:0016021 P96408 NC_000962.3 Mycobrowser_v5 CDS 276058 277764 . + 0 Rv0231 fadE4 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE4 Rv0231, (MTCY08D5.27), len: 568 aa. Probable fadE4, acyl-CoA dehydrogenase, similar to many e.g. O29752 acyl-CoA dehydrogenase (ACD-3) from Archaeoglobus fulgidus (576 aa), FASTA scores: opt: 1788, E(): 0, (51.0% identity in 577 aa overlap); ACDB_BACSU|P45857 acyl-CoA dehydrogenase from Bacillus subtilis (379 aa), FASTA scores: opt: 232, E(): 2.2e- 08, (21.6% identity in 291 aa overlap). P96414 No lipid metabolism P96414 P96414 GO:0055114,GO:0003995 1.3.99.- P96414 NC_000962.3 Mycobrowser_v5 CDS 279605 281140 . - 0 Rv0234c gabD1 Involved in 4-aminobutyrate (GABA) degradation pathway [catalytic activity: succinate semialdehyde + NAD(P)(+) + H(2)O = succinate + NAD(P)H]. Succinate-semialdehyde dehydrogenase [NADP+] dependent (SSDH) GabD1 Rv0234c, (MTCY08D5.30c), len: 511 aa. gabD1, succinate-semialdehyde dehydrogenase [NADP+] dependent, equivalent to AL022486|MLCB1883_6 probable aldehyde dehydrogenase from Mycobacterium leprae (457 aa), FASTA scores: opt: 2617, E(): 0, (85.7% identity in 455 aa overlap). Also highly similar to Q55585|GABD|SLR0370 probable succinate-semialdehyde dehydrogenase from Synechocystis sp. strain PCC 6803 (454 aa), FASTA scores: opt: 1676, E(): 0, (55.8% identity in 455 aa overlap); and similar to others e.g. GABD_ECOLI|P25526 succinate-semialdehyde dehydrogenase from Escherichia coli (482 aa), FASTA scores: opt: 929, E(): 0, (36.5% identity in 452 aa overlap); etc. Note that similar to other cytosolic aldehyde dehydrogenases with EC number: 1.2.1.3. Also similar to Rv0768|aldA semialdehyde dehydrogenase from Mycobacterium tuberculosis (489 aa); and gabD2|Rv1731|MTCY04C12.16 possible succinate-semialdehyde dehydrogenase [NADP+] dependent from Mycobacterium tuberculosis (518 aa). Contains PS00070 aldehyde dehydrogenases cysteine active site. Belongs to the aldehyde dehydrogenases family. Could start at different site by homology. Note that previously known as gabD2. P9WNX9 No intermediary metabolism and respiration P71989 P9WNX9 GO:0009013,GO:0006099,GO:0055114 1.2.1.16 P9WNX9 NC_000962.3 Mycobrowser_v5 CDS 298116 298862 . - 0 Rv0247c Rv0247c Involved in interconversion of fumarate and succinate (aerobic respiration) [catalytic activity: succinate + acceptor = fumarate + reduced acceptor]. Probable succinate dehydrogenase [iron-sulfur subunit] (succinic dehydrogenase) Rv0247c, (MTV034.13c), len: 248 aa. Probable succinate dehydrogenase, iron-sulfur subunit, highly similar to CAC44313.1|AL596043 putative succinate dehydrogenase iron-sulfur subunit from Streptomyces coelicolor (259 aa); and similar to iron-sulphur protein subunits of fumarate reductase or succinate dehydrogenases from many bacteria e.g. NP_147618.1|7521083|B72691 fumarate reductase iron-sulfur protein from Aeropyrum pernix (305 aa); NP_069516.1|2649932|AAB90556.1|AE001057 succinate dehydrogenase iron-sulfur subunit B (sdhB) from Archaeoglobus fulgidus (236 aa); etc. Also similar to Q10761|FRDB_MYCTU|7431693|F70762 fumarate reductase iron-sulfur protein from Mycobacterium tuberculosis (247 aa), FASTA scores: opt: 358, E():1e-16, (31.3% identity in 214 aa overlap). Contains PS00197 2Fe-2S ferredoxins, iron-sulfur binding region signature. Note that succinate dehydrogenase forms generally part of an enzyme complex containing four subunits: a flavoprotein (Rv0248c ?), an iron-sulfur (Rv0247c ?), and two hydrophobic anchor proteins (Rv0249c ?). O53669 No intermediary metabolism and respiration O53669 O53669 GO:0009055,GO:0046872,GO:0055114,GO:0000104,GO:0006099,GO:0051537 1.3.99.1 O53669 NC_000962.3 Mycobrowser_v5 CDS 1547832 1549151 . + 0 Rv1375 Rv1375 Function unknown Conserved hypothetical protein Rv1375, (MTCY02B12.09), len: 439 aa. Conserved hypothetical protein, similar to hypothetical proteins from several organisms e.g. Q52871|U39409 Rhizobium leguminosarum (420 aa), FASTA scores: E(): 2e-30, (34.4% identity in 378 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). P9WF27 No conserved hypotheticals P71803 P9WF27 P9WF27 NC_000962.3 Mycobrowser_v5 CDS 287186 288352 . + 0 Rv0237 lpqI Unknown Probable conserved lipoprotein LpqI Rv0237, (MTV034.03), len: 388 aa. Probable lpQI, conserved lipoprotein, equivalent to AL022486|MLCB1883_8|T44873 probable secreted hydrolase from Mycobacterium leprae (387 aa), FASTA scores: opt: 1831, E(): 0, (73.3% identity in 390 aa overlap). Also similar to other lipoproteins and various hydrolases e.g. P40406|2126897|YBBD_BACSU|I39839 hypothetical 70.6 KDA lipoprotein from Bacillus subtilis (642 aa); P48823|HEXA_ALTSO beta-hexosaminidase a precursor from alteromonas SP. (598 aa), FASTA scores: opt: 415, E(): 5.8e-17, (31.2% identity in 343 aa overlap); PCC6803|P74340 beta-glucosidase from Synechocystis sp. (538 aa), FASTA scores: opt: 414, E(): 6.1e-17, (30.6 identity in 320 aa overlap). Contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. L7N6B0 No cell wall and cell processes Q7DA74 L7N6B0 GO:0004553,GO:0005975 L7N6B0 NC_000962.3 Mycobrowser_v5 CDS 290665 292029 . - 0 Rv0242c fabG4 Involved in the fatty acid biosynthesis pathway (first reduction step) [catalytic activity: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH]. Probable 3-oxoacyl-[acyl-carrier protein] reductase FabG4 (3-ketoacyl-acyl carrier protein reductase) Rv0242c, (MTV034.08c), len: 454 aa. Probable fabG4, 3-oxoacyl-[acyl-carrier protein] reductase, equivalent to 3063883|CAA18568.1|AL022486|MLCB1883_13|T44878 3-oxoacyl-[acyl-carrier protein] reductase homolog from Mycobacterium leprae (454 aa), FASTA scores: opt: 2486, E(): 0, (84.8% identity in 454 aa overlap). C-terminal part highly similar to many FabG proteins e.g. U39441|VHU3944 1_2 from Vibrio harveyi (244 aa), FASTA scores: opt: 562, E(): 3.4e-28, (40.2% identity in 241 aa overlap); U91631|PAU91631_3 from Pseudomonas aeruginosa (247 aa), FASTA scores: opt: 584, E(): 1.5e-29, (44.4% identity in 241 aa overlap). Has N-terminal extension of ~200 aa and C-terminal part contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. I6Y778 No lipid metabolism 3M1L,3Q6I O53665 I6Y778 GO:0055114,GO:0004316 1.1.1.100 I6Y778 NC_000962.3 Mycobrowser_v5 CDS 293798 295633 . - 0 Rv0244c fadE5 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE5 Rv0244c, (MTV034.10c), len: 611 aa. Probable fadE5, acyl-CoA dehydrogenase, equivalent to AL022486|MLCB1883_15 from Mycobacterium leprae (611 aa), FASTA scores: opt: 3598, E(): 0, (89.4% identity in 611 aa overlap). Also highly similar to AL0211|MTV007.14 from Mycobacterium tuberculosis (609 aa), FASTA scores: opt: 2576, E(): 0, (64.6% identity in 611 aa overlap); and to various other bacterial proteins described as putative acyl-CoA dehydrogenases e.g. AE0010|AE001025_6 from Archaeoglobus fulgidus (387 aa), FASTA scores: opt: 229, E(): 6.8e-08, (29.8% identity in 409 aa overlap); etc. O53666 No lipid metabolism O53666 O53666 GO:0003995,GO:0055114,GO:0050660 1.3.99.- O53666 NC_000962.3 Mycobrowser_v5 CDS 306374 307858 . - 0 Rv0255c cobQ1 Involved in cobalamin biosynthesis Probable cobyric acid synthase CobQ1 Rv0255c, (MTV034.21c), len: 494 aa. Probable cobQ1, cobyric acid synthase, similar to many e.g. Z46611|RCBLUGNS_8 cobyric acid synthase from R.capsulatus (483 aa), FASTA scores: opt: 1239, E(): 0, (47.1% identity in 493 aa overlap); P29932|COBQ_PSEDE cobyric acid synthase from Pseudomonas denitrificans (484 aa), FASTA scores: opt: 1168, E():0, (44.9% identity in 490 aa overlap); etc. Belongs to the COBB/COBQ family, COBQ subfamily. Note that previously known as cobQ. P9WP95 No intermediary metabolism and respiration P0A532 P9WP95 GO:0015420,GO:0006541,GO:0009236 P9WP95 NC_000962.3 Mycobrowser_v5 CDS 612255 612605 . + 0 Rv0520 Rv0520 Could cause methylation. Possible methyltransferase/methylase (fragment) Rv0520, (MTCY20G10.10), len: 116 aa. Possible fragment of methyltransferase (possibly first part), highly similar to part of several methyltransferases e.g. Q43445|U43683 S-adenosyl-L-methionine:DELTA24-sterol-C-methyltransferase from Glycine max (Soybean)(367 aa), FASTA scores: opt: 190, E(): 2.3e-12, (39.2% identity in 74 aa overlap). Also some similarity to MTCY19G5_5 from Mycobacterium tuberculosis. Possibly continues as Rv0521 but we can find no frameshift to account for this. O33365 No intermediary metabolism and respiration O33365 O33365 GO:0008168,GO:0008152 2.1.1.- O33365 NC_000962.3 Mycobrowser_v5 CDS 312759 314168 . - 0 Rv0261c narK3 Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate. Responsible for the translocation of the substrate across the membrane. Probable integral membrane nitrite extrusion protein NarK3 (nitrite facilitator) Rv0261c, (MTCY06A4.05c), len: 469 aa. Probable nirK3, nitrite extrusion protein, integral membrane protein possibly member of major facilitator superfamily (MFS), equivalent to AAB41700.1|U72744 nitrite extrusion protein from Mycobacterium fortuitum (471 aa); and 2342627|CAB11406.1|Z98741|T44908 nitrite extrusion protein homolog from Mycobacterium leprae (517 aa; longer in N-terminus). Also similar to other nitrite extrusion proteins e.g. NARK_ECOLI|P10903|B1223 nitrite extrusion protein 1 from Escherichia coli strain K12 (463 aa), FASTA scores: opt: 755, E(): 0, (35.0% identity in 466 aa overlap). Belongs to the nark/NASA family of transporters. P95218 No cell wall and cell processes P95218 P95218 GO:0055085 P95218 NC_000962.3 Mycobrowser_v5 CDS 316511 317503 . - 0 Rv0265c Rv0265c Thought to be involved in iron transport across the membrane (import). Probable periplasmic iron-transport lipoprotein Rv0265c, (MTCY06A4.09c), len: 330 aa. Probable iron-transport lipoprotein, most similar to T36412|5763945|CAB53324.1|AL109974 probable iron-siderophore binding lipoprotein from Streptomyces coelicolor (350 aa); and (N-terminus may be incorrect) to T14166|3560508|AAC82551.1|AF027770 fxuD protein from Mycobacterium smegmatis (420 aa), FASTA scores: opt: 385, E(): 1.5e-16, (32.3% identity in 232 aa overlap). Also similar to AAB97475.1|U02617 DtxR/iron regulated lipoprotein precursor from Corynebacterium diphtheriae (355 aa); FECB_ECOLI|P15028 iron(III) dicitrate-binding periplasmic protein (300 aa), FASTA scores: opt: 191, E(): 2.3e-05, (26.5% identity in 196 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Note that previously known as fecB2. L7N6B2 No cell wall and cell processes Q7DA51 L7N6B2 GO:0005381,GO:0006827 L7N6B2 NC_000962.3 Mycobrowser_v5 CDS 321331 322722 . + 0 Rv0267 narU Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate. Responsible for the translocation of the substrate across the membrane. Probable integral membrane nitrite extrusion protein NarU (nitrite facilitator) Rv0267, (MTCY06A4.11), len: 463 aa. Probable narU, nitrite extrusion protein, integral membrane protein possibly member of major facilitator superfamily (MFS), similar to other nitrite extrusion proteins e.g. NARU_ECOLI|P37758 nitrite extrusion protein 2 from Escherichia coli (462 aa), FASTA scores: opt: 630, E(): 4.4e-33, (38.9% identity in 463 aa overlap); and NARK_ECOLI|P10903|B1223 nitrite extrusion protein 1 from Escherichia coli strain K12 (463 aa), FASTA scores: opt: 607, E(): 1.3e-31, (42.0% identity in 457 aa overlap). Also similar to Rv0261c, Rv2329c, Rv1737c, and to MLCB22_25 from Mycobacterium leprae (517 aa), FASTA score: (35.1 identity in 459 aa overlap). Belongs to the nark/NASA family of transporters. P95224 No cell wall and cell processes P95224 P95224 GO:0015698,GO:0016021,GO:0055085,GO:0015103 P95224 NC_000962.3 Mycobrowser_v5 CDS 366150 372764 . - 0 Rv0304c PPE5 Function unknown PPE family protein PPE5 Rv0304c, (MTCY63.9c), len: 2204 aa. PPE5, Member of the Mycobacterium tuberculosis PE family (PPE, MPTR), similar to others e.g. Z95324|MTY13E10_16 from Mycobacterium tuberculosis (1443 aa), FASTA scores: E(): 0, (50.6% identity in 1403 aa overlap); Y04H_MYCTU|Q10778 from Mycobacterium tuberculosis (734 aa), FASTA scores: opt: 989, E(): 0, (42.3% identity in 522 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q6MX49 No PE/PPE Q6MX49 Q6MX49 Q6MX49 NC_000962.3 Mycobrowser_v5 CDS 330422 331003 . + 0 Rv0274 Rv0274 Function unknown Conserved protein Rv0274, (MTV035.02), len: 193 aa. Conserved protein, highly similar to AAK25058.1|AE005973 conserved hypothetical protein from Caulobacter crescentus (174 aa). Shows also some similarity to others hypothetical proteins e.g. AJ002571|BSAJ2571_7 from Bacillus subtilis (316 aa), FASTA scores: opt: 138, E(): 0.033, (27.1% identity in 133 aa overlap). Previous hits with Q56415|M85195 fosfomycin-resistance protein from serratia marcescens (141 aa), FASTA scores: opt: 82, E(): 1.1e -08, (29.1% identity in 151 aa overlap). Contains PS00082 Extradiol ring-cleavage dioxygenases signature near C-terminus. May belong to the vicinal-oxygen-chelate (VOC) superfamily of metalloenzymes (See Rawat et al., 2003). O53680 No conserved hypotheticals O53680 O53680 GO:0008198,GO:0055114,GO:0016702,GO:0019439 O53680 NC_000962.3 Mycobrowser_v5 CDS 333437 336310 . - 0 Rv0278c PE_PGRS3 Function unknown PE-PGRS family protein PE_PGRS3 Rv0278c, (MTV035.06c), len: 957 aa. PE_PGRS3, Member of the Mycobacterium tuberculosis PE family (see citation below), PGRS subfamily of gly-rich proteins, similar to many e.g. Z95890|MTCY28_25|Rv1759c from Mycobacterium tuberculosis (914 aa), FASTA scores: opt: 3849, E(): 0, (67.8% identity in 903 aa overlap). Contains PS00583 pfkB family of carbohydrate kinases signature 1. P9WIG3 No PE/PPE P56877 P9WIG3 P9WIG3 NC_000962.3 Mycobrowser_v5 CDS 340998 341906 . + 0 Rv0281 Rv0281 Possible methyltransferase Possible S-adenosylmethionine-dependent methyltransferase Rv0281, (MTV035.09), len: 302 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), member of Mycobacterium tuberculosis protein family that includes Rv0726c, Rv0731c, Rv3399, Rv1729c, etc. MTCY31_23 (325 aa), FASTA scores: opt: 1386, E(): 0, (69. 1% identity in 301 aa overlap). Contains possible N-terminal signal sequence. P9WFI9 No lipid metabolism O53686 P9WFI9 GO:0008168 2.1.1.- P9WFI9 NC_000962.3 Mycobrowser_v5 CDS 342130 344025 . + 0 Rv0282 eccA3 Function unknown ESX conserved component EccA3. ESX-3 type VII secretion system protein. Rv0282, (MTV035.10), len: 631 aa. eccA3, esx conserved component, ESX-3 type VII secretion system protein, similar to Y14967|MLCB628.18c hypothetical protein from Mycobacterium leprae (573 aa), FASTA scores: opt: 916, E(): 0, (38.7% identity in 568 aa overlap). Also similar to Mycobacterium tuberculosis proteins e.g. Z94121|MTY15F10.26 (619 aa), FASTA scores: opt: 743, E(): 0, (29.9% identity in 612 aa overlap). Member of CFXQ, CBXP family - 9 members in Mycobacterium tuberculosis. Contains PS00017 ATP/GTP-binding site motif A (P-loop). P9WPI3 No cell wall and cell processes O53687 P9WPI3 GO:0005737,GO:0017111,GO:0005524 P9WPI3 NC_000962.3 Mycobrowser_v5 CDS 364044 364469 . + 0 Rv0301 vapC2 Unknown Possible toxin VapC2 Rv0301, (MTCY63.06), len: 141 aa. Possible vapC2, toxin, part of toxin-antitoxin (TA) operon with Rv0300, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to others in Mycobacterium tuberculosis e.g. Rv2757c, Rv0229c, Rv2546, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WFB9 No virulence, detoxification, adaptation 3H87 O07228 P9WFB9 P9WFB9 NC_000962.3 Mycobrowser_v5 CDS 372820 375711 . - 0 Rv0305c PPE6 Function unknown PPE family protein PPE6 Rv0305c, (MTCY63.10c), len: 963 aa. PPE6, Member of the Mycobacterium tuberculosis PE family (PPE, MPTR), similar to others e.g. Y04H_MYCTU|Q10778 from Mycobacterium tuberculosis (734 aa), FASTA scores: opt: 1340, E(): 0, (40.9% identity in 815 aa overlap). Q6MX48 No PE/PPE Q6MX48 Q6MX48 Q6MX48 NC_000962.3 Mycobrowser_v5 CDS 352149 353036 . + 0 Rv0289 espG3 Function unknown ESX-3 secretion-associated protein EspG3 Rv0289, (MTV035.17), len: 295 aa. EspG3, ESX-3 secretion-associated protein, equivalent to CAC32061.1|AL583926 possible DNA-binding protein from Mycobacterium leprae (289 aa); and showing some similarity to Rv3866|G70656|CAB06238.1|Z94121|MTCY15F10.23 from Mycobacterium tuberculosis (276 aa), FASTA scores: opt: 149, E(): 0.0035, (27.7% identity in 289 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WJC7 No cell wall and cell processes P9WJC7 P9WJC7 NC_000962.3 Mycobrowser_v5 CDS 364605 365237 . + 0 Rv0302 Rv0302 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably TetR/AcrR-family) Rv0302, (MTCY63.07), len: 210 aa. Probable transcription regulatory protein, TetR family (see citation below), with its N-terminus similar to N-terminus of several repressors and regulatory proteins of TetR/AcrR family e.g. ACRR_ECOLI|P34000 potential acraB operon repressor from Escherichia coli (215 aa), FASTA scores: opt: 172, E(): 3.1e-05, (22.7% identity in 194 aa overlap). Also similar in N-terminus to N-terminus of MTCY07A7.24 hypothetical regulator from Mycobacterium tuberculosis FASTA score: (38.7% identity in 62 aa overlap). Contains probable helix-turn helix motif from aa 35-56 (Score 1728, +5.07 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07229 No regulatory proteins O07229 O07229 GO:0006350,GO:0003700,GO:0006355 O07229 NC_000962.3 Mycobrowser_v5 CDS 354498 355883 . + 0 Rv0291 mycP3 Thought to have proteolytic activity. Probable membrane-anchored mycosin MycP3 (serine protease) (subtilisin-like protease) (subtilase-like) (mycosin-3) Rv0291, (MTV035.19), len: 461 aa. Probable mycP3, membrane-anchored serine protease (mycosin) (see Brown et al., 2000), similar to several others in mycobacteria e.g. Z94121|MTY15F10_28|Rv1796 from Mycobacterium tuberculosis (446 aa), FASTA scores: opt: 1168, E(): 0, (44.6% identity in 453 aa overlap); Rv3886c; Rv3883c; Rv3449; and Y14967|MLCB628_4|MLCB628.04 from Mycobacterium leprae (446 aa), FASTA scores: opt: 1159, E(): 0, (43.5 identity in 446 aa overlap). Has signal sequence and hydrophobic stretch at C-terminus, followed by short positively charged segment, that seems to act as a membrane anchor. Contains PS00137 Serine proteases, subtilase family, histidine active site signature. Belongs to peptidase family S8 (also known as the subtilase family), pyrolysin subfamily. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). O53695 No intermediary metabolism and respiration O53695 O53695 GO:0004252,GO:0006508 3.4.21.- O53695 NC_000962.3 Mycobrowser_v5 CDS 359758 361155 . - 0 Rv0296c Rv0296c Function unknown; probably involved in cellular metabolism. Probable sulfatase Rv0296c, (MTCY63.01c, MTV035.24c), len: 465 aa. Probable sulfatase, possibly an aryl-/steryl-sulfatase or a sulfamidase (sulfohydrolase) (sulphamidase). Similar to various hydrolases e.g. AAG41945.1|AF304053_1|AF304053 heparan N-sulfatase from Mus musculus (502 aa); NP_061292.1|6851181|AAF29460.1|AF153827_1|AF153827 N-sulfoglucosamine sulfohydrolase (sulfamidase) (sulphamidase) from Mus musculus (502 aa); AAG17206.1|AF217203_1|AF217203 heparan sulfate sulfamidase from Canis familiaris (507 aa); P08842|STS_HUMAN|1360652 steryl-sulfatase precursor (steroid sulfatase) (steryl-sulfate sulfohydrolase) (arylsulfatase C) (ASC) from Homo sapiens (583 aa); ARSB_FELCA|P33727 arylsulfatase B precursor (535 aa), FASTA scores: opt: 231, E(): 1.7e-08, (30.3% identity in 261 aa overlap). Also similarity with 4 others sulfatases in Mycobacterium tuberculosis. Contains sulfatases signature 1 (PS00523). Note that previously known as atsG. Q6MX51 No intermediary metabolism and respiration Q6MX51 Q6MX51 GO:0008484,GO:0008152 3.-.-.- Q6MX51 NC_000962.3 Mycobrowser_v5 CDS 361334 363109 . + 0 Rv0297 PE_PGRS5 Function unknown PE-PGRS family protein PE_PGRS5 Rv0297, (MTCY63.02), len: 591 aa. PE_PGRS5, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to others e.g. Y03A_MYCTU|Q10637 from Mycobacterium tuberculosis (603 aa), FASTA scores: opt: 1884, E(): 0, (53.7% identity in 635 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q6MX50 No PE/PPE Q6MX50 Q6MX50 Q6MX50 NC_000962.3 Mycobrowser_v5 CDS 385173 385943 . - 0 Rv0317c glpQ2 Glycerophosphoryl diester phosphodiesterase hydrolyzes deacylated phospholipids to G3P and the corresponding alcohols [catalytic activity: a glycerophosphodiester + H(2)O = an alcohol + SN-glycerol 3-phosphate]. Possible glycerophosphoryl diester phosphodiesterase GlpQ2 (glycerophosphodiester phosphodiesterase) Rv0317c, (MTCY63.22c), len: 256 aa (start uncertain, chosen by homology). Possible glpQ2, glycerophosphoryl diester phosphodiesterase, similar to others e.g. E75317|6459876|AAF11631.1|AE002044_4 glycerophosphoryl diester phosphodiesterase from Deinococcus radiodurans (285 aa); P10908|UGPQ_ECOLI from Escherichia coli (247 aa), FASTA scores: opt: 220, E(): 5.2e-07, (28.0% identity in 250 aa overlap). Also similar to MTCY01A6.27 from Mycobacterium tuberculosis FASTA score: (27.5% identity in 247 aa overlap). O07244 No intermediary metabolism and respiration O07244 O07244 GO:0008889,GO:0006629,GO:0006071 3.1.4.46 O07244 NC_000962.3 Mycobrowser_v5 CDS 391352 392032 . + 0 Rv0324 Rv0324 Possibly involved in transcriptional mechanism. Possible transcriptional regulatory protein (possibly ArsR-family) Rv0324, (MTCY63.29), len: 226 aa. Possible transcriptional regulator, arsR family, with its N-terminus similar to the N-terminus of other DNA-binding proteins e.g. P30346|MERR_STRLI probable mercury resistance operon from Streptomyces lividans (125 aa), FASTA scores: opt: 154, E(): 0.002, (32.2% identity in 90 aa overlap), and its C-terminal part similar to hypothetical bacterial proteins e.g. P54510|YQHL_BACSU hypothetical 14.6 kDa protein from Bacillus subtilis (126 aa), FASTA scores: opt: 159, E(): 0.00097, (35.5% identity in 76 aa overlap). Most similar to AJ005575|SPE005575_2 ORF1 required for antibiotic production from Streptomyces peucetius (226 aa), FASTA scores: opt: 816, E(): 0, (60.7% identity in 211 aa overlap). Also similar in C-terminus to MTCY164.26 molybdopterin biosynthesis moeb protein from Mycobacterium tuberculosis FASTA score: (36.8% identity in 114 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O08446 No regulatory proteins O08446 O08446 GO:0006355,GO:0008272,GO:0004792,GO:0006350,GO:0003700,GO:0005622 O08446 NC_000962.3 Mycobrowser_v5 CDS 396201 397367 . + 0 Rv0331 Rv0331 Function unknown; probably involved in cellular metabolism. Possible dehydrogenase/reductase Rv0331, (MTCY63.36), len: 388 aa. Possible dehydrogenase/reductase, similar to various dehydrogenases/reductases e.g. NP_103779.1|14022957|BAB49565.1|AP002999 flavoprotein reductase from Mesorhizobium loti (377 aa); NP_147681.1 predicted NAD(FAD)-dependent dehydrogenase from Aeropyrum pernix (381 aa); DHSU_CHRVI|Q06530 sulfide dehydrogenase (431 aa), FASTA scores: opt: 347, E(): 6.8e-15, (25.6% identity in 348 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07255 No intermediary metabolism and respiration O07255 O07255 GO:0016491,GO:0055114 1.-.-.- O07255 NC_000962.3 Mycobrowser_v5 CDS 398658 399524 . + 0 Rv0334 rmlA dTDP-L-rhamnose biosynthesis within the O antigen biosynthesis pathway of lipopolysaccharide biosynthesis [catalytic activity: dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-glucose]. Alpha-D-glucose-1-phosphate thymidylyltransferase RmlA (dTDP-glucose synthase) (dTDP-glucose pyrophosphorylase) Rv0334, (MTCY279.01), len: 288 aa. RmlA (alternate gene name: rfbA), alpha-D-glucose-1-phosphate thymidylyl-transferase (see citations below), equivalent to CAC32020.1|AL583925 glucose-1-phosphate thymidyltransferase from Mycobacterium leprae (288 aa). Also highly similar to others e.g. AAG29804.1|AF235050 glucose-1-phosphate thymidylyltransferase from Streptomyces rishiriensis (296 aa); RBA1_ECOLI|P37744 glucose-1-phosphate thymidylyltransferase from Escherichia coli strain K12 (293 aa), FASTA scores: opt: 1199, E(): 0, (62.0% identity in 284 aa overlap). Belongs to the glucose-1-phosphate thymidylyltransferase family. P9WH13 No intermediary metabolism and respiration P72017 P9WH13 GO:0008879,GO:0046872,GO:0045226 2.7.7.24 P9WH13 NC_000962.3 Mycobrowser_v5 CDS 403193 405841 . - 0 Rv0338c Rv0338c Function unknown; probably involved in cellular metabolism. Probable iron-sulfur-binding reductase Rv0338c, (MTCY279.05c), len: 882 aa. Probable iron-sulphur-binding reductase, possibly membrane-bound, equivalent to CAC32018.1|AL583925 probable iron-sulphur-binding reductase from Mycobacterium leprae (880 aa). Also highly similar to others e.g. T36608|5019323|CAB44376.1|AL078610 probable iron-sulfur-binding reductase from Streptomyces coelicolor (760 aa), FASTA scores: opt: 1658, E(): 0, (49.9% identity in 772 aa overlap); BAB07521.1|AP001520 iron-sulphur-binding reductase from Bacillus halodurans (700 aa). Contains PS00070 Aldehyde dehydrogenases cysteine active site and two of PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature. First of several possible start sites chosen. O33268 No intermediary metabolism and respiration O33268 O33268 GO:0051536,GO:0055114,GO:0016491,GO:0009055 1.-.-.- O33268 NC_000962.3 Mycobrowser_v5 CDS 405950 408448 . - 0 Rv0339c Rv0339c Could be involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0339c, (MTCY279.06c), len: 832 aa. Possible transcriptional regulator, showing very weak similarity with parts of others. Contains PS00017 ATP/GTP-binding site motif A (P-loop); and probable helix-turn helix motif from aa 778-799 (Score 1041, +2.73 SD). O33269 No regulatory proteins O33269 O33269 GO:0006355,GO:0043565,GO:0006350,GO:0003700,GO:0000156,GO:0000160,GO:0005622 O33269 NC_000962.3 Mycobrowser_v5 CDS 409362 410801 . + 0 Rv0341 iniB Unknown Isoniazid inductible gene protein IniB Rv0341, (MTCY13E10.01), len: 479 aa. IniB, isoniazid-inducible gene, (see citations below). Protein very Gly-, Ala-rich, similar to cell wall proteins e.g. P27483|GRP_ARATH glycine-rich cell wall structural protein from A.thaliana (338 aa), FASTA scores: opt: 532, E(): 5.2e-13, (39.3% identity in 321 aa overlap). MEME-mast analysis shows similarity to product of upstream gene, Rv0340. P9WJ97 No cell wall and cell processes P9WJ97 EMB,INH NC_000962.3 Mycobrowser_v5 CDS 412757 414238 . + 0 Rv0343 iniC Unknown Isoniazid inductible gene protein IniC Rv0343, (MTCY13E10.03), len: 493 aa. IniC, isoniazid-inducible gene, (see citations below). Shows slight similarity to P40983|YOR6_THER8 hypothetical protein (402 aa), FASTA scores: opt: 196, E(): 2.6e-05, (25.9% identity in 228 aa overlap). Also some similarity to upstream ORF Rv0342|iniA. Contains (PS00017) ATP/GTP-binding site motif A (P-loop). Note that the iniA gene is also induced by the antibiotic ethambutol, an agent that inhibits cell wall biosynthesis by a mechanism that is distinct from isoniazid. P9WJ95 No cell wall and cell processes O06294 P9WJ95 GO:0003924,GO:0005525 EMB,INH P9WJ95 NC_000962.3 Mycobrowser_v5 CDS 415502 416965 . - 0 Rv0346c ansP2 Thought to be involved in transport of L-asparagine across the membrane. Responsible for the translocation of the substrate across the membrane. Possible L-asparagine permease AnsP2 (L-asparagine transport protein) Rv0346c, (MTCY13E10.06c), len: 487 aa. Possible ansP2, L-asparagine permease, integral membrane protein belonging to family containing many amino acid permeases, highly similar to G467030|B2126_F2_85|NP_301937.1|NC_002677 probable L-asparagine permease from Mycobacterium leprae (498 aa); and NP_301938.1|NC_002677 probable L-asparagine permease from Mycobacterium leprae (505 aa). Also highly similar to others e.g. P77610|ANSP_ECOLI L-asparagine permease from Escherichia coli strain K-12 (499 aa). Also highly similar to ANSP1|Rv2127|MT2186|MTCY261_22|O33261 probable L-asparagine permease from Mycobacterium tuberculosis (489 aa), FASTA score: (72.1% identity in 473 aa overlap). And shows some similarity to MTCY3G12.14 from Mycobacterium tuberculosis. Belongs to the amino acid permease family (APC family). Note that previously known as aroP2. P9WQM7 No cell wall and cell processes P0A4W0 P9WQM7 GO:0006865,GO:0016021,GO:0005886,GO:0055085,GO:0015171 NC_000962.3 Mycobrowser_v5 CDS 422452 423639 . + 0 Rv0352 dnaJ1 Acts as a co-chaperone. Stimulates, jointly with GRPE|Rv0351, the ATPase activity of DNAK|Rv0350. Seems to be regulated negatively by HSPR (Rv0353 product). Probable chaperone protein DnaJ1 Rv0352, (MTCY13E10.12), len: 395 aa. Probable dnaJ1, chaperone protein, equivalent to AAA25363.1|M95576 DNA J heatshock protein from Mycobacterium leprae (389 aa). Also highly similar to others. Contains both DnaJ signatures (PS00636, and PS00637). Belongs to the DNAJ family. Cofactor: binds two zinc ions per monomer. Note that sequence differs from DNAJ_MYCTU|P07881 due to a frameshift at the N-terminus. Note that previously known as dnaJ. P9WNV9 No virulence, detoxification, adaptation P0A548 P9WNV9 GO:0006260,GO:0005737,GO:0031072,GO:0046872,GO:0006457,GO:0009408,GO:0051082,GO:0005524 P9WNV9 NC_000962.3 Mycobrowser_v5 CDS 435471 436769 . - 0 Rv0357c purA Involved in AMP biosynthesis (first committed step). Plays an important role in the de novo pathway of purine nucleotide biosynthesis [catalytic activity: GTP + imp + L-aspartate = GDP + phosphate + adenylosuccinate]. Probable adenylosuccinate synthetase PurA (imp--aspartate ligase) (ADSS) (ampsase) Rv0357c, (MTCY13E10.19c), len: 432 aa. Probable purA, adenylosuccinate synthase, equivalent to AL023514|MLCB4_13 from adenylosuccinate synthetase Mycobacterium leprae (432 aa), FASTA scores: opt: 2555, E(): 0, (87.9% identity in 431 aa overlap). Also highly similar to many bacterial adenylosuccinates synthetases e.g. P12283|PURA_ECOLI adenylosuccinates synthetase from Escherichia coli (431 aa), FASTA scores: E(): 0, (51.1% identity in 425 aa overlap); etc. Belongs to the adenylosuccinate synthetase family. P9WHN3 No intermediary metabolism and respiration P65880 P9WHN3 GO:0004019,GO:0005737,GO:0000287,GO:0006164,GO:0005525 6.3.4.4 P9WHN3 NC_000962.3 Mycobrowser_v5 CDS 453230 454372 . - 0 Rv0376c Rv0376c Function unknown Conserved hypothetical protein Rv0376c, (MTV036.11c), len: 380 aa. Conserved hypothetical protein, highly similar to T35481|4008539|CAA22508.1|AL034492|SC6C5.10 hypothetical protein from Streptomyces coelicolor (395 aa); and AAK64260.1|AF373840_20 ORF377 hypothetical CoxI from Arthrobacter nicotinovorans (377 aa). And similar to other conserved hypothetical proteins e.g. NP_101963.1|14021136|BAB47749.1|AP002994 hypothetical protein from Mesorhizobium loti (245 aa). Note that C-terminus shows similarity with C-termini of CAB76248.1|X82447|COXF CoxF protein from Pseudomonas/Oligotropha carboxidovorans (280 aa); CAB76250.1|X82447|COXI CoxI protein from Pseudomonas/Oligotropha carboxidovorans (330 aa); and AJ224684|BJAJ4684_6 cooxS from Bradyrhizobium japonicum (176 aa), FASTA scores: E(): 1.9e-17, (47.1% identity in 138 aa overlap). Also some partial similarity with AJ224684|BJAJ4684_5 cooxS from Bradyrhizobium japonicum (107 aa), FASTA scores: opt: 321, E(): 4.2e-14, (53.3% identity in 92 aa overlap); E1184330|Z99120|YURF YURF protein from Bacillus subtilis (330 aa), FASTA scores: opt: 170, E(): 2.9e- 16, (27.5% identity in 345 aa overlap). Also similar to downstream ORF Rv0372c from Mycobacterium tuberculosis (251 aa), FASTA scores: E(): 2.1e-06, (30.7% identity in 277 aa overlap). O53711 No conserved hypotheticals O53711 O53711 O53711 NC_000962.3 Mycobrowser_v5 CDS 439871 441253 . + 0 Rv0362 mgtE Thought to be involved in Mg2+ transport. Responsible for the translocation of the substrate across the membrane. Possible Mg2+ transport transmembrane protein MgtE Rv0362, (MTCY13E10.24), len: 460 aa. Possible mgtE, magnesium (Mg2+) transport transmembrane protein; C-terminal region is highly similar to MGTE|G780283 putative Mg2+ transporter from Providencia stuarti (314 aa), FASTA scores: E(): 0, (47.2% identity in 307 aa overlap) (N-terminus extends approx. 150 aa further upstream compared to P. stuarti ORF). Also similar in part to others e.g. AAK20879.1|AF334760_1|AF334760 putative Mg2+ transporter from Aeromonas hydrophila (455 aa); NP_231292.1|NC_002505 magnesium transporter from Vibrio cholerae (451 aa); NP_102305.1|NC_002678 Mg2+ transport protein from Mesorhizobium loti (454 aa); etc. Also similar to Rv1232c|MTV006.04c from Mycobacterium tuberculosis (435 aa). Extended hydrophobic segment spanning last 130 residues. Belongs to the MgtE family. O06312 No cell wall and cell processes O06312 O06312 GO:0006812,GO:0016021,GO:0008324 O06312 NC_000962.3 Mycobrowser_v5 CDS 444844 445233 . - 0 Rv0367c Rv0367c Unknown Hypothetical protein Rv0367c, (MTV036.02c), len: 129 aa. Hypothetical unknown protein. O53702 No conserved hypotheticals O53702 O53702 NC_000962.3 Mycobrowser_v5 CDS 445314 446525 . - 0 Rv0368c Rv0368c Function unknown Conserved hypothetical protein Rv0368c, (MTV036.03c), len: 403 aa. Conserved hypothetical protein, showing some similarity to AJ224684|BJAJ4684_4 cooxS protein from Bradyrhizobium japonicum (422 aa), FASTA scores: opt: 341, E(): 4.3e-13, (27.4% identity in 387 aa overlap); Rv2425c|MTCY428_22 hypothetical protein from Mycobacterium tuberculosis FASTA score: (30.7% identity in 238 aa overlap). Contains PS00213 Lipocalin signature. O53703 No conserved hypotheticals O53703 O53703 O53703 NC_000962.3 Mycobrowser_v5 CDS 448040 448633 . - 0 Rv0371c Rv0371c Function unknown Conserved hypothetical protein Rv0371c, (MTV036.06c), len: 197 aa. Conserved hypothetical protein, similar to other hypothetical proteins e.g. AL132824|SCAH10.09c|CAB60163.1|AL132824 hypothetical protein from Streptomyces coelicolor (207 aa), FASTA scores: opt: 247, E(): 4.5e-09, (32.3% identity in 195 aa overlap). Also weak similarity with YURE|D70017|Z99120|BSUB0017_134 hypothetical protein yurE from Bacillus subtilis (197 aa), FASTA scores: opt: 217, E(): 2.5e-08, (27.0% identity in 174 aa overlap). I6WY86 No conserved hypotheticals 2WE9,2WEE,2YES I6WY86 I6WY86 NC_000962.3 Mycobrowser_v5 CDS 448630 449385 . - 0 Rv0372c Rv0372c Function unknown Conserved hypothetical protein Rv0372c, (MTV036.07c), len: 251 aa. Conserved hypothetical protein, showing some similarity with CAB76248.1|X82447|COXF CoxF protein from Pseudomonas/Oligotropha carboxidovorans (280 aa); AJ224684|BJAJ4684_6 cooxS from Bradyrhizobium japonicum (176 aa), FASTA scores: opt: 186, E(): 1.6e-05, (41.1% identity in 95 aa overlap). Also similar to upstream ORF Rv0376c from Mycobacterium tuberculosis (380 aa), FASTA scores: E(): 6.8e-07, (31.0% identity in 277 aa overlap). O53707 No conserved hypotheticals O53707 O53707 O53707 NC_000962.3 Mycobrowser_v5 CDS 449404 451803 . - 0 Rv0373c Rv0373c Function unknown; probably involved in cellular metabolism [catalytic activity: CO + H(2)O + acceptor = CO(2) + reduced acceptor]. Probable carbon monoxyde dehydrogenase (large chain) Rv0373c, (MTV036.08c), len: 799 aa. Probable carbon monoxide dehydrogenase, large chain, highly similar to others e.g. AAD00363.1| U80806|CUTL carbon monoxide dehydrogenase large subunit CutL protein from Hydrogenophaga pseudoflava (803 aa); S49124|509391|CAA54902.1|X77931|1094915|2107180C|CUTA carbon-monoxide dehydrogenase large chain (cut operon) from Pseudomonas thermocarboxydovorans (842 aa); C56279|809566|CAA57829.1|X82447|OCCOXMSL4_3|COXL carbon-monoxide dehydrogenase large chain (cluster coxMSL) from Pseudomonas/Oligotropha carboxydovorans (809 aa), FASTA scores: opt: 2484, E(): 0, (56.0% identity in 804 aa overlap); etc. O53708 No intermediary metabolism and respiration O53708 O53708 GO:0005507,GO:0030151,GO:0055114,GO:0018492 1.2.99.2 O53708 NC_000962.3 Mycobrowser_v5 CDS 612598 612903 . + 0 Rv0521 Rv0521 Could cause methylation Possible methyltransferase/methylase (fragment) Rv0521, (replaces MTCY20G10.11), len: 101 aa. Possible fragment of methyltransferase (possibly second part), highly similar to C-terminus of several methyltransferases e.g. AAF87203.1|AF216282 sarcosine-dimethylglycine methyltransferase from Halorhodospira halochloris (279 aa). Possibly continuation of Rv0520 but we can find no frameshift to account for this. L7N6C0 No intermediary metabolism and respiration L7N6C0 GO:0008168 2.1.1.- NC_000962.3 Mycobrowser_v5 CDS 457841 458380 . - 0 Rv0382c pyrE Involved in pyrimidine biosynthesis (at the fifth step) [catalytic activity: orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate]. Probable orotate phosphoribosyltransferase PyrE (OPRT) (oprtase) Rv0382c, (MTV036.17c), len: 179 aa. Probable pyrE, orotate phosphoribosyltransferase, equivalent to CAC32004.1|AL583925 probable purine/pyrimidine phosphoribosyltransferase from Mycobacterium leprae (179 aa). Also highly similar to many others e.g. T36540|4753874|CAB42037.1|AL049754|SCH10.28c probable orotate phosphoribosyltransferase from Streptomyces coelicolor (182 aa); H69115|2622996|AAB86326.1|AE000938_10|MTH1860 probable orotate phosphoribosyltransferase from Methanobacterium thermoautotrophicum (180 aa), FASTA scores: opt: 389, E(): 2.7e-20, (40.7% identity in 172 aa overlap); O08359|PYRE_SULAC|2065444|CAA73352.1|Y12822 orotate phosphoribosyltransferase from Sulfolobus acidocaldarius (197 aa); etc. Note that also similar to other puridine 5'-monophosphate synthases (umpA genes; UMP synthases), generally in N-terminus that corresponds to orotate phosphoribosyltransferase activity. Contains PS00589 PTS HPR component serine phosphorylation site signature. Belongs to the purine/pyrimidine phosphoribosyltransferase family. Note that previously known as umpA. Nucleotide position 458282 in the genome sequence has been corrected, A:G resulting in Y33Y. P9WHK9 No intermediary metabolism and respiration P0A5U0 P9WHK9 GO:0004588,GO:0006221,GO:0009116 2.4.2.10 P9WHK9 NC_000962.3 Mycobrowser_v5 CDS 459456 462002 . - 0 Rv0384c clpB Thought to be an ATPase subunit of an intracellular ATP-dependent protease. Seems to be regulated positively by sigh (Rv3223c product) and negatively by HSPR (Rv0353 product). Probable endopeptidase ATP binding protein (chain B) ClpB (ClpB protein) (heat shock protein F84.1) Rv0384c, (MTV036.19c), len: 848 aa. Probable clpB (alternate gene name: htpM), endopeptidase ATP-binding protein, chain B, equivalent to AC32007.1|AL583925 heat shock protein from Mycobacterium leprae (848 aa). Also highly similar to others e.g. P53532|CLPB_CORGL|1163118|AAB49540.1|U43536|CGU43536_1 CLPB protein (heat-inducible expression) from Corynebacterium glutamicum (852 aa), FASTA scores: opt: 4113, E(): 0, (74.5% identity in 846 aa overlap); T36551|4753885|CAB42048.1|AL049754|clpB|SCOEDB|SCH10.39c probable ATP-dependent proteinase ATP-binding chain from Streptomyces coelicolor (853 aa); P03815|CLPB_ECOLI|1788943|AAC75641.1|AE000345 CLPB protein (heat shock protein F84.1) from Escherichia coli strains K12 and O157:H7 (857 aa); etc. Also similar to Rv3596c|ClpC from Mycobacterium tuberculosis. Contains PS00870 and PS00871 Chaperonins clpA/B signatures and two PS000017 ATP/GTP-binding site motives a (P-loop). Belongs to the CLPA/CLPB family. Contains probable coiled-coil domain from aa 411-503. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P9WPD1 No virulence, detoxification, adaptation P63288 P9WPD1 GO:0005737,GO:0017111,GO:0005515,GO:0019538,GO:0006950,GO:0005524 P9WPD1 NC_000962.3 Mycobrowser_v5 CDS 468335 469594 . + 0 Rv0389 purT Involved in third step (first of two transformylation reactions) in de novo purine biosynthesis. This is an alternative enzyme to the PURN|Rv0956 gar transformylase (5'-phosphoribosylglycinamide formyltransferase). Catalyzes two reactions: the first one is the production of beta-formyl glycinamide ribonucleotide (gar) from formate, ATP and beta gar; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [catalytic activity: formate + ATP + 5'-phospho-ribosylglycinamide = 5'-phosphoribosyl-N-formylglycinamide + ADP + pyrophosphate]. Probable phosphoribosylglycinamide formyltransferase 2 PurT (GART 2) (gar transformylase 2) (5'-phosphoribosylglycinamide transformylase 2) (formate-dependent gar transformylase) Rv0389, (MTCY04D9.01, MTV036.24), len: 419 aa. Probable purT, phosphoribosylglycinamide formyltransferase 2, similar to others e.g. P33221|PURT_ECOLI|B1849 phosphoribosylglycinamide formyltransferase 2 from Escherichia coli strain K-12 (391 aa), FASTA scores: opt: 481, E(): 1.3e-22, (40.1% identity in 379 aa overlap); etc. Belongs to the PurK / PurT family. Cofactor: magnesium. P95197 No intermediary metabolism and respiration P95197 P95197 GO:0016742,GO:0000287,GO:0009152,GO:0005524 2.1.2.- P95197 NC_000962.3 Mycobrowser_v5 CDS 483977 485734 . + 0 Rv0404 fadD30 Function unknown, but involved in lipid degradation. Fatty-acid-AMP ligase FadD30 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) Rv0404, (MTCY04D9.17-MTCY22G10.00), len: 585 aa. fadD30, fatty-acid-AMP synthetase, similar to many e.g. MBU75685_1|AAB52538.1|U75685 acyl-CoA synthase from Mycobacterium bovis (582 aa); MASC_MYCLE|P54200 masc protein from Mycobacterium leprae (372 aa), FASTA scores: opt: 888, E(): 0, (44.2% identity in 342 aa overlap). Also similar to Y06J_MYCTU|Q10976 hypothetical 67.9 kDa protein (626 aa), FASTA scores: opt: 1463, E(): 0, (42.4% identity in 568 aa overlap). P9WQ57 No lipid metabolism P95213 P9WQ57 GO:0008152,GO:0016874 6.2.1.- P9WQ57 NC_000962.3 Mycobrowser_v5 CDS 472781 474106 . + 0 Rv0393 Rv0393 Function unknown Conserved 13E12 repeat family protein Rv0393, (MTCY04D9.05), len: 441 aa. Member of Mycobacterium tuberculosis 13E12 repeat family of conserved proteins, similar to many e.g. Rv1148c, Rv1945, Rv3467, Rv0336|MTCY279_3 (503 aa), FASTA scores: E(): 0, (61.1% identity in 347 aa overlap). P95201 No insertion seqs and phages P95201 P95201 NC_000962.3 Mycobrowser_v5 tRNA 924110 924183 . + 0 MTB000012 aspT - tRNA-Asp (GTC) aspT, tRNA-Asp, anticodon gtc, length = 74 No stable RNAs NC_000962.3 Mycobrowser_v5 tRNA 1025321 1025393 . - 0 MTB000014 argT - tRNA-Arg (CCT) argT, tRNA-Arg, anticodon cct, length = 73 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 478566 479753 . - 0 Rv0400c fadE7 Function unknown, but involved in lipid degradation. Acyl-CoA dehydrogenase FadE7 Rv0400c, (MTCY04D9.12c), len: 395 aa. Probable fadE7, acyl-CoA dehydrogenase, similar to many e.g. CAC12923.1|AL445403 putative acyl CoA dehydrogenase from Streptomyces coelicolor (397 aa); G624219 glutaryl-CoA dehydrogenase precursor (438 aa), FASTA scores: opt: 1161, E(): 0, (48.1% identity in 391 aa overlap); etc. P95208 No lipid metabolism P95208 P95208 GO:0003995,GO:0055114,GO:0050660 1.3.99.- NC_000962.3 Mycobrowser_v5 CDS 1896475 1896867 . + 0 Rv1671 Rv1671 Unknown Probable membrane protein Rv1671, (MTV047.06), len: 130 aa. Probable membrane protein. Weak similarity to mercuric transport proteins. O53918 No cell wall and cell processes O53918 O53918 NC_000962.3 Mycobrowser_v5 CDS 490783 491793 . + 0 Rv0407 fgd1 Catalyzes oxidation of glucose-6-phosphate to 6-phosphogluconolactone using coenzyme F420 (an *-hydroxy-5-deazaflavin derivative) as the electron acceptor. F420-dependent glucose-6-phosphate dehydrogenase Fgd1 Rv0407, (MTCY22G10.03), len: 336 aa. fgd1, F420-dependent glucose-6-phosphate dehydrogenase, equivalent to others from Mycobacteria e.g. AAD38165.1|AF152394_3 from Mycobacterium avium (336 aa), FASTA scores: opt: 2082, E(): 0, (89.9% identity in 336 aa overlap); AL023514|MLCB 4_7 from Mycobacterium leprae (336 aa), FASTA scores: opt: 2069, E(): 0, (89.0% identity in 336 aa overlap). Also similar to other dehydrogenases e.g. CAA77276.1|Y18730 F420-dependent alcohol dehydrogenase from Methanofollis liminatans (330 aa). Also similar to many proteins from Mycobacterium tuberculosis e.g. Rv0953c, Rv0791c, etc. Note that previously known as fgd. P9WNE1 No intermediary metabolism and respiration 3B4Y,3C8N P96253 P9WNE1 GO:0016491,GO:0055114 1.-.-.- P9WNE1 NC_000962.3 Mycobrowser_v5 CDS 491786 493858 . + 0 Rv0408 pta Involved at the last step (of two) in the conversion of acetate to acetyl-CoA [catalytic activity: acetyl-CoA + phosphate = CoA + acetyl phosphate]. Probable phosphate acetyltransferase Pta (phosphotransacetylase) Rv0408, (MTCY22G10.04), len: 690 aa. Probable pta, phosphate acetyltransferase, highly similar to others e.g. PTA_ECOLI|P39184|11279789|JX0357|B2297 phosphate acetyltransferase from Escherichia coli strain K12 (713 aa), FASTA scores: opt: 1303, E(): 0, (38.0% identity in 718 aa overlap); etc. Belongs to the phosphate acetyltransferase and butyryltransferase family. P9WHP1 No intermediary metabolism and respiration P96254 P9WHP1 GO:0008152,GO:0008959,GO:0005737 2.3.1.8 P9WHP1 NC_000962.3 Mycobrowser_v5 CDS 499713 500366 . + 0 Rv0413 mutT3 Possibly involved in the go system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite DA and DC residues of template DNA with almost equal efficiency thus leading to A.T to G.C transversions. MutT specifically degrades 8-oxo-dGTP to the monophosphate [catalytic activity: 8-oxo-dGTP + H2O = 8-oxo-dGMP + pyrophosphate]. Possible mutator protein MutT3 (7,8-dihydro-8-oxoguanine-triphosphatase) (8-oxo-dGTPase) (dGTP pyrophosphohydrolase) Rv0413, (MTCY22G10.10), len: 217 aa. Possible mutT3, mutator protein (see citation below), showing some similarity with e.g. MUTT_PROVU|P32090 mutator mutt protein from Proteus vulgaris (112 aa), FASTA scores: opt: 151, E(): 0.0008, (40.7% identity in 59 aa overlap). Seems to belong to the NUDIX hydrolase family. P9WIX9 No information pathways P96259 P9WIX9 GO:0006260,GO:0016787,GO:0046872,GO:0006281 3.6.1.- P9WIX9 NC_000962.3 Mycobrowser_v5 CDS 500350 501018 . - 0 Rv0414c thiE Involved in thiamine biosynthesis. Condenses 4-methyl-5-(beta-hydroxyethyl)-thiazole monophosphate (THZ-P) and 4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-pp) to form thiamine monophosphate (TMP) [catalytic activity: 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-4-methyl-5-(2-phosphonooxyethyl)-thiazole = diphosphate + thiamine monophosphate]. Thiamine-phosphate pyrophosphorylase ThiE (TMP pyrophosphorylase) (TMP-PPASE) (thiamine-phosphate synthase) Rv0414c, (MTCY22G10.11c), len: 222 aa. thiE, thiamin phosphate pyrophosphorylase, equivalent to Q9ZBL5|AL035159|MLCB1450_17 probable thiamine-phosphate pyrophosphorylase from Mycobacterium leprae (235 aa), FASTA scores: opt: 1095, E(): 0, (78.0% identity in 223 aa overlap). Also similar to others e.g. T34974|5689976|CAB52013.1|AL109663 probable thiamin phosphate pyrophosphorylase from Streptomyces coelicolor (223 aa); THIE_ECOLI|P30137 thie protein from Escherichia coli strain K12 (211 aa), FASTA scores: opt: 275, E(): 7.8e-12, (37.8% identity in 196 aa overlap); etc. Belongs to the TMP-PPASE family. P9WG75 No intermediary metabolism and respiration 3O63 P66916 P9WG75 GO:0009228,GO:0004789,GO:0000287 2.5.1.3 P9WG75 NC_000962.3 Mycobrowser_v5 CDS 2584486 2584755 . + 0 Rv2312 Rv2312 Unknown Hypothetical protein Rv2312, (MTCY3G12.22c), len: 89 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLB9 No conserved hypotheticals P9WLB9 NC_000962.3 Mycobrowser_v5 CDS 502366 503124 . + 0 Rv0417 thiG Involved in thiamine biosynthesis. Required for the synthesis of the thiazole moiety of thiamine. Probable thiamin biosynthesis protein ThiG (thiazole biosynthesis protein) Rv0417, (MTCY22G10.14), len: 252 aa. Probable thiG, thiamin biosynthesis protein, equivalent to AL035159|MLCB1450_20|T44741|THIG probable thiamin biosynthesis protein from Mycobacterium leprae (261 aa), FASTA scores: opt: 1380, E(): 0, (86.8% identity in 250 aa overlap). Also highly similar to others e.g. SCOEDB|SC6E10.03|T35490|THIG probable thiazole biosynthesis protein from Streptomyces coelicolor (264 aa); F82761|9105679|AAF83593.1|AE003919_4|XF0783|THIG thiamin biosynthesis protein thiG from Xylella fastidiosa (275 aa); P30139|THIG_ECOLI|7448315|B65206|409790|AAC43089.1|U00006 THIG protein thiamin biosynthesis protein from Escherichia coli strain K-12 (281 aa); etc. Belongs to the THIG family. P9WG73 No intermediary metabolism and respiration P96263 P9WG73 GO:0016829,GO:0009228,GO:0005737 4.-.-.- P9WG73 NC_000962.3 Mycobrowser_v5 CDS 503496 504998 . + 0 Rv0418 lpqL Function unknown; hydrolyzes peptides and/or proteins. Probable lipoprotein aminopeptidase LpqL Rv0418, (MTCCY22G10.15), len: 500 aa. Probable lpqL, lipoprotein aminopeptidase, similar to others e.g. B83278|9949035|AAG06327.1|AE004720_3|AE004720|PA2939 probable aminopeptidase from Pseudomonas aeruginosa (536 aa); P80561|APX_STRGR|SGAP|S66427 aminopeptidase from Streptomyces griseus (284 aa) (homology only with C-terminus of Rv0418); P37302|APE3_YEAST|1077010|A54134 aminopeptidase Y from Saccharomyces cerevisiae (537 aa); etc. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. P96264 No cell wall and cell processes P96264 P96264 GO:0006508,GO:0004177 3.4.11.- P96264 NC_000962.3 Mycobrowser_v5 CDS 508582 510225 . - 0 Rv0423c thiC Involved in thiamine biosynthesis. Required for the synthesis of the hydromethylpyrimidine (HMP) moiety of thiamine (4-amino-2-methyl-5-hydroxymethylpyrimidine). Probable thiamine biosynthesis protein ThiC Rv0423c, (MTCY22G10.20c), len: 547 aa. Probable thiC, thiamin biosynthesis protein, equivalent to Q9ZBL0|THIC_MYCLE|11279601|T44743|AL035159|MLCB1450_22 thiamine biosynthesis protein from Mycobacterium leprae (547 aa), FASTA scores: opt: 3283, E(): 0, (90.1% identity in 547 aa overlap). Also highly similar to others e.g. P45740|THIC_BACSU thiamin biosynthesis protein from Bacillus subtilis (590 aa), FASTA scores: opt: 2295, E(): 0, (65.2% identity in 580 aa overlap); P30136|THIC_ECOLI THIC protein from Escherichia coli strain K12 (631 aa), FASTA scores: opt: 2141, E(): 0, (62.1% identity in 568 aa overlap); etc. Belongs to the ThiC family. P9WG79 No intermediary metabolism and respiration P66911 P9WG79 GO:0016829,GO:0046872,GO:0009228,GO:0051539 4.-.-.- P9WG79 NC_000962.3 Mycobrowser_v5 CDS 510702 515321 . - 0 Rv0425c ctpH Metal cation-transporting ATPase; possibly catalyzes the transport of an undetermined metal cation with the hydrolysis of ATP [catalytic activity: ATP + H(2)O + undetermined metal cation(in) = ADP + phosphate + undetermined metal cation(out)]. Possible metal cation transporting P-type ATPase CtpH Rv0425c, (MTCY22G10.22c), len: 1539 aa. Possible ctpH, metal cation-transporting P-type ATPase (transmembrane protein), showing some similarity with CAA17934.1|AL022118|13093871|CAC32203.1|AL583926 putative cation-transporting ATPase from Mycobacterium leprae (1609 aa). Also similar to others ATPases e.g. AE000873_1 cation-transporting P-ATPase from Methanobacterium thermoautotrop (844 aa), FASTA score: (30.5% identity in 827 aa overlap); AB69720.1|AL137166 putative transport ATPase from Streptomyces coelicolor (1472 aa); etc. C-terminal region similar to other ATPases from Mycobacterium tuberculosis e.g. Y05Q_MYCTU|Q10900 putative cation-transporting ATPase C (855 aa), FASTA scores: opt: 770, E(): 5.3e-32, (44.9% identity in 820 aa overlap). Nucleotide position 511518 in the genome sequence has been corrected, T:G resulting in I1268I. P96271 No cell wall and cell processes P96271 P96271 GO:0006754,GO:0016021,GO:0015693,GO:0015444,GO:0005524 3.6.1.- P96271 NC_000962.3 Mycobrowser_v5 CDS 516017 516892 . - 0 Rv0427c xthA Involved in base excision repair. Apurinic-apyrimidinic endonuclease. Supposed to remove the damaged DNA at cytosines and guanines by cleaving at the 3' side of the AP site by a beta-elimination reaction. Possibly exhibites 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase and ribonuclease H activities [catalytic activity: degradation of double-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing 5'-phosphomononucleotides]. Probable exodeoxyribonuclease III protein XthA (exonuclease III) (EXO III) (AP endonuclease VI) Rv0427c, (MTCY22G10.24c), len: 291 aa. Probable xthA (alternate gene name: xth), exodeoxyribonuclease III protein (see citation below), similar to others e.g. EX3_ECOLI|P09030 exodeoxyribonuclease III from Escherichia Coli strain K12 (268 aa), FASTA scores: opt: 360, E(): 1.2e-17, (29.3% identity in 270 aa overlap); etc. Belongs to the AP/EXOA family of DNA repair enzymes. P96273 No information pathways P96273 P96273 GO:0006281,GO:0004519,GO:0008853,GO:0005622,GO:0003677 3.1.11.2 P96273 NC_000962.3 Mycobrowser_v5 CDS 710782 711009 . + 0 Rv0616A vapB29 Unknown Possible antitoxin VapB29 Rv0616A, len: 75 aa. Possible vapB29, antitoxin, part of toxin-antitoxin (TA) operon with Rv0617, see Arcus et al. 2005. Similar to many others in M. tuberculosis e.g. Rv2530A (74 aa) 35.9% identity in 78 aa overlap P9WJ37 No virulence, detoxification, adaptation P9WJ37 NC_000962.3 Mycobrowser_v5 CDS 516895 517803 . - 0 Rv0428c Rv0428c Acetylation, substrate unknown GCN5-related N-acetyltransferase Rv0428c, (MTCY22G10.25c), len: 302 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain in C-terminal part. See Vetting et al. 2005. P96274 No intermediary metabolism and respiration P96274 NC_000962.3 Mycobrowser_v5 CDS 520324 521454 . + 0 Rv0433 Rv0433 Function unknown Conserved hypothetical protein Rv0433, (MTCY22G10.30), len: 376 aa. Conserved hypothetical protein, similar to other hypothetical proteins e.g. P77213|YBDK_ECOLI hypothetical 41.7 KD protein from Escherichia coli strain K12 (372 aa), FASTA scores: opt: 555, E(): 2e-30, (28.2% identity in 365 aa overlap). P9WPK9 No conserved hypotheticals P64693 P9WPK9 GO:0004357,GO:0006750,GO:0005524 6.3.-.- P9WPK9 NC_000962.3 Mycobrowser_v5 CDS 521514 522167 . + 0 Rv0434 Rv0434 Function unknown Conserved hypothetical protein Rv0434, (MTCY22G10.31), len: 217 aa. Conserved hypothetical protein, similar to AE002052_2 from Deinococcus radiodurans (213 aa), FASTA scores: opt: 258, E(): 4e-10, (31.9% identity in 213 aa overlap); SYCSLRB_122|Q55701 hypothetical 24.5 kDa protein from Synechocystis (214 aa), FASTA scores: opt: 156, E(): 0.00041, (28.4% identity in 204 aa overlap); MXABSGA_1|LON2_MYXXA|P36774 ATP-dependent protease la 2 from Myxococcus xanthus (826 aa), FASTA scores: opt: 160, E(): 0.00068, (28.4% identity in 197 aa overlap); etc. P96280 No conserved hypotheticals P96280 P96280 GO:0006508,GO:0004176 P96280 NC_000962.3 Mycobrowser_v5 CDS 522347 524533 . - 0 Rv0435c Rv0435c ATPase; possibly catalyzes the transport of undetermined substrate with hydrolyse of ATP [catalytic activity: ATP + H(2)O + undetermined substrate(in) = ADP + phosphate + undetermined substrate(out)]. Putative conserved ATPase Rv0435c, (MTCY22G10.32c), len: 728 aa. Putative conserved ATPase, similar to others e.g. SAV_SULAC|Q07590 sav protein involved in cell division from sulfolobus acidocaldarius (780 aa), FASTA scores: opt: 897, E(): 0, (34.5% identity in 693 aa overlap); NP_148637.1|7435761|B72479 transitional endoplasmic reticulum ATPase from Aeropyrum pernix (699 aa); etc. Also similar to Rv3610c and Rv2115c from Mycobacterium tuberculosis. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00674 AAA-protein family signature. P96281 No cell wall and cell processes P96281 P96281 GO:0051301,GO:0017111,GO:0005524 3.6.1.- P96281 NC_000962.3 Mycobrowser_v5 CDS 525387 526082 . - 0 Rv0437c psd Function unknown, but involved in lipid metabolism [catalytic activity: phosphatidyl-L-serine = phosphatidylethanolamine + CO(2)]. Possible phosphatidylserine decarboxylase Psd (PS decarboxylase) Rv0437c, (MTV037.01c), len: 231 aa (start uncertain). Possible psd, phosphatidylserine decarboxylase , equivalent to CAC29819.1|AL583918 conserved hypothetical protein from Mycobacterium leprae (243 aa); and highly similar to MLCB1450.11|T44729|4154044|CAA22695.1|AL035159 hypothetical protein from Mycobacterium leprae (202 aa), FASTA score: (74.6% identity in 197 aa overlap). Also similar to other phosphatidylserine decarboxylases e.g. NP_108058.1|14027249|BAB54203.1|AP003012 phosphatidylserine decarboxylase from Mesorhizobium loti (232 aa); AAK86872|g15156090|AGR_C_1963 phosphatidylserine decarboxylase from Agrobacterium tumefaciens (244 aa); AAG12422.1|AY005137|Psd phosphatidylserine decarboxylase from Chlorobium tepidum (216 aa); etc. P9WHQ5 No lipid metabolism P67547 P9WHQ5 GO:0006646,GO:0004609,GO:0048037 4.1.1.65 P9WHQ5 NC_000962.3 Mycobrowser_v5 CDS 527379 528314 . - 0 Rv0439c Rv0439c Function unknown; possibly involved in cellular metabolism. Probable dehydrogenase/reductase Rv0439c, (MTV037.03c), len: 311 aa. Probable dehydrogenase/reductase, equivalent to AL035159|MLCB1450_6|T44727 probable oxidoreductase from Mycobacterium leprae (304 aa), FASTA scores: opt: 1360, E(): 0, (69.2% identity in 302 aa overlap). Also highly similar to various oxidoreductases, generally dehydrogenases/reductases e.g. PA5031|C83017|9951320|AAG08416.1|AE004916_5|AE004916 probable short chain dehydrogenase from Pseudomonas aeruginosa (309 aa); Q03326|OXIR_STRAT probable oxidoreductase from Streptomyces antibioticus (298 aa), FASTA scores: opt: 400, E(): 1.2e-18, (34.6% identity in 298 aa overlap); etc. O53726 No intermediary metabolism and respiration O53726 O53726 GO:0055114,GO:0016491 1.-.-.- O53726 NC_000962.3 Mycobrowser_v5 CDS 528608 530230 . + 0 Rv0440 groEL2 Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions. 60 kDa chaperonin 2 GroEL2 (protein CPN60-2) (GroEL protein 2) (65 kDa antigen) (heat shock protein 65) (cell wall protein A) (antigen A) Rv0440, (MTV037.04), len: 540 aa. GroEL2 (alternate gene names: groL2, groEL-2, hsp65, hsp60), 60 kDa chaperonin 2 (see Shinnick 1987). Purified 65 kDa antigen can elicit a strong delayed-type hypersensitivity reaction in experimental animals infected with M. tuberculosis. This protein is one of the major immunoreactive proteins of the mycobacteria. This antigen contains epitopes that are common to various species of mycobacteria. Contains PS00296 Chaperonins cpn60 signature. Belongs to the chaperonin (HSP60) family. Phosphorylated in vitro by PknJ|Rv2088 (See Arora et al., 2010). P9WPE7 No virulence, detoxification, adaptation 1SJP,3RTK P0A520 P9WPE7 GO:0005737,GO:0042026,GO:0051082,GO:0005524 P9WPE7 NC_000962.3 Mycobrowser_v5 CDS 533833 534396 . - 0 Rv0445c sigK Involved in transcription mechanism. The sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released. Alternative RNA polymerase sigma factor SigK Rv0445c, (MTV037.09c), len: 187 aa. sigK, alternative RNA polymerase sigma factor (see citations below), highly similar to others e.g. 5531433|CAB50938.1|AL096849|T36745 probable RNA polymerase sigma factor from Streptomyces coelicolor (185 aa); NP_105607.1|14024791|BAB51393.1|AP003005 RNA polymerase sigma factor from Mesorhizobium loti (179 aa); 1654108|AAB17906.1|U11283|A58883 probable transcription initiation factor sigma E from Rhodobacter phaeroides (168 aa), FASTA scores: opt: 299, E(): 2e-14, (32.7% identity in 168 aa overlap); Q45585|SIGW_BACSU RNA polymerase sigma factor SIGW from Bacillus subtilis (187 aa), FASTA scores: opt: 213, E(): 2.9e-08, (26.8% identity in 179 aa overlap); etc. P9WGH7 No information pathways O53730 P9WGH7 GO:0016987,GO:0003700,GO:0006352,GO:0006355 P9WGH7 NC_000962.3 Mycobrowser_v5 CDS 535224 536507 . - 0 Rv0447c ufaA1 Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge [catalytic activity: S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid]. Probable cyclopropane-fatty-acyl-phospholipid synthase UfaA1 (cyclopropane fatty acid synthase) (CFA synthase) Rv0447c, (MTV037.11c), len: 427 aa (start uncertain). Probable ufaA1, cyclopropane-fatty-acyl-phospholipid synthase, similar to others e.g. NP_102178.1|14021351|BAB47964.1|AP002994 cyclopropane-fatty-acyl-phospholipid synthase from Mesorhizobium loti (378 aa); B82240|9655593|AAF94281.1|AE004192 cyclopropane-fatty-acyl-phospholipid synthase from Vibrio cholerae (432 aa); P30010|CFA_ECOLI cyclopropane-fatty-acyl-phospholipid synthase from Escherichia coli strain K-12 (382 aa); X55704|PPLPD_3 LPD-3 from P.putida (394 aa), FASTA scores: opt: 556, E(): 2.8e-30, (33.3% identity in 387 aa overlap); AE0005|HPAE000557_9 from Helicobacter pylori (389 aa), FASTA scores: opt: 539, E(): 3.9e-29, (34.3% identity in 382 aa overlap). O53732 No lipid metabolism O53732 O53732 GO:0008610,GO:0008825 2.1.1.79 O53732 NC_000962.3 Mycobrowser_v5 CDS 630040 631686 . + 0 Rv0538 Rv0538 Unknown Possible conserved membrane protein Rv0538, (MTCY25D10.17), len: 548 aa. Possible conserved membrane protein. Middle region highly similar to AAB63811.1|AF009829|MBE4863a|O32850 unknown protein from Mycobacterium bovis (295 aa) possible transmembrane protein with a repetitive proline, threonine-rich region at C-terminus. O06404 No cell wall and cell processes O06404 O06404 NC_000962.3 Mycobrowser_v5 CDS 538588 541491 . - 0 Rv0450c mmpL4 Unknown. Thought to be involved in fatty acid transport. Probable conserved transmembrane transport protein MmpL4 Rv0450c, (MTV037.14c), len: 967 aa. Probable mmpL4, conserved transmembrane transport protein (see citations below), member of RND superfamily, equivalent to U1740V|P54881|YV34_MYCLE hypothetical 105.2 kDa protein from Mycobacterium leprae (959 aa), FASTA scores: opt: 5051, E(): 0, (78.4% identity in 962 aa overlap). Also highly similar to other proteins from Mycobacterium tuberculosis e.g. Z83860|MTCY98.08 (962 aa), FASTA scores: opt: 3917, E(): 0, (61.3% identity in 950 aa overlap), MTCY20G9.34, etc. Contains PS00211 ABC transporters family signature. Belongs to the MmpL family. P9WJV3 No cell wall and cell processes O53735 P9WJV3 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 545375 545821 . - 0 Rv0455c Rv0455c Function unknown Conserved protein Rv0455c, (MTV037.19c), len: 148 aa. Conserved protein, equivalent to CAC31896.1|AL583925 possible secreted protein from Mycobacterium leprae (153 aa). Has hydrophobic stretch at N-terminus. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). O53740 No conserved hypotheticals O53740 O53740 NC_000962.3 Mycobrowser_v5 CDS 554882 556306 . - 0 Rv0465c Rv0465c Possibly involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv0465c, (MTV038.09c), len: 474 aa. Probable transcriptional regulator, highly similar to AC44331.1|AL596102 putative DNA-binding protein from Streptomyces coelicolor (489 aa); and similar to several hypothetical proteins and others transcriptional regulators. Some similarity in N-terminal region (1-100 aa) with repressors e.g. P06153|RPC_BPPH1 immunity repressor protein (144 aa), FASTA scores: opt: 130, E(): 0.084,(27.0% identity in 100 aa overlap). Very similar to Rv1129c|Z95585|MTCY22G8.18c from Mycobacterium tuberculosis (486 aa), FASTA scores: opt: 1475, E(): 0, (47.4% identity in 468 aa overlap). Contains probable helix-turn-helix motif at aa 19-40 (1827, +5.41 SD). P9WMI1 No regulatory proteins O53750 P9WMI1 GO:0043565,GO:0006350,GO:0045449 P9WMI1 NC_000962.3 Mycobrowser_v5 CDS 557527 558813 . + 0 Rv0467 icl1 Involved in glyoxylate bypass (at the first step), an alternative to the tricarboxylic acid cycle (in bacteria, plants, and fungi) [catalytic activity: isocitrate = succinate + glyoxylate]. Involved in the persistence in the host. Isocitrate lyase Icl (isocitrase) (isocitratase) Rv0467, (MTV038.11), len: 428 aa. Icl1, isocitrate lyase (see citations below), highly similar to many, closest to Z29367|RFISCILY_1 from R. fascians (429 aa), FASTA scores: opt: 2359, E(): 0, (80.7% identity in 429 aa overlap). Belongs to the isocitrate lyase family. Has 2-methyl-isocitrate lyase (MCL) activity in M. tuberculosis Erdman (See Munoz-Elias et al., 2006; Gould et al., 2006). Predicted possible vaccine candidate (See Zvi et al., 2008). P9WKK7 No intermediary metabolism and respiration 1F61,1F8I,1F8M P0A5H3 P9WKK7 GO:0006097,GO:0004451,GO:0006099,GO:0005737 4.1.3.1 P9WKK7 NC_000962.3 Mycobrowser_v5 CDS 560848 561711 . - 0 Rv0470c pcaA Involved in the mycolic acid modification or synthesis; essential for the cyclopropanation function. Required for cording and mycolic acid cyclopropane ring synthesis in the cell wall. Mycolic acid synthase PcaA (cyclopropane synthase) Rv0470c, (MTV038.14), len: 287 aa. PcaA (previously known as umaA2), mycolic acid synthase (cyclopropane synthase) (see citations below), equivalent to CAC31976.1|AL583925 Mycolic acid synthase from Mycobacterium leprae (295 aa); and highly similar to S72886|B2168_F3_130|467038|AAA17222.1|U00018 hypothetical protein from Mycobacterium leprae (308 aa); Q11195|CFA1_MYCTU cyclopropane-fatty-acyl-phospholipid synthase 1 (cyclopropane mycolic acid synthase 1) (287 aa) (see Glickman et al., 2000); U27357|MTU27357_1 cyclopropane mycolic acid synthase from Mycobacterium tuberculosis (287 aa), FASTA scores: opt: 1415, E(): 0, (72.8% identity in 287 aa overlap); and related enzymes e.g. MTCY20H10.25c|Z92772|MTY20H10_26 (287 aa), FASTA scores: opt: 1387, E(): 0, (72.5% identity in 287 aa overlap). P9WPB3 No lipid metabolism 1L1E Q7D9R5 P9WPB3 GO:0008610,GO:0008825 2.-.-.- P9WPB3 NC_000962.3 Mycobrowser_v5 CDS 562723 563427 . - 0 Rv0472c Rv0472c Possibly involved in transcriptional mechanism. Probable transcriptional regulatory protein (possibly TetR-family) Rv0472c, (MTV038.16c), len: 234 aa. Probable regulatory protein, possibly TetR family, equivalent to CAC31974.1|AL583925 possible TetR-family transcriptional regulator from Mycobacterium leprae (233 aa). Also similar to CAC01492.1|AL391017 putative transcriptional regulatory protein from Streptomyces coelicolor (218 aa); and CAC01371.1|AL390975 putative TetR-family transcriptional regulator from Streptomyces coelicolor (228 aa). Also similar to AL0212|MTV012_65 from Mycobacterium tuberculosis (246 aa), FASTA scores: opt: 327, E(): 1.8e-15, (31.0% identity in 232 aa overlap); and Z95120|MTCY07D11.18c (228 aa), FASTA scores: opt: 190, E(): 4.4e-06, (23.1% identity in 186 aa overlap). Contains probable helix-turn-helix doimain at aa 45-66 (Score 1429, +4.05 SD). P9WMD9 No regulatory proteins O53757 P9WMD9 GO:0006350,GO:0003700,GO:0006355 P9WMD9 NC_000962.3 Mycobrowser_v5 CDS 565797 566396 . + 0 Rv0475 hbhA Required for extrapulmonary dissemination. Mediates adherence to epithelial cells by binding to sulfated glycoconjugates present at the surface of these cells; binds heparin, dextran sulfate, fucoidan and chondroitin sulfate. Promotes hemagglutination of erythrocytes of certain host species. Induces mycobacterial aggregation. Iron-regulated heparin binding hemagglutinin HbhA (adhesin) Rv0475, hbhA (MTCY20G9.01), len: 199 aa. HbhA, iron-regulated heparin-binding hemagglutinin (see citations below), equivalent to CAC31971.1|AL583925 possible hemagglutinin from Mycobacterium leprae (188 aa). Contains possible N-terminal signal sequence and K-a-rich region at C-terminus: subcellular location: surface associated. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WIP9 No cell wall and cell processes P9WIP9 GO:0009986,GO:0008201,GO:0009405,GO:0007155 P9WIP9 NC_000962.3 Mycobrowser_v5 CDS 568964 569806 . - 0 Rv0480c Rv0480c Function unknown; hydrolytic enzyme probably involved in cellular metabolism. Possible amidohydrolase Rv0480c, (MTCY20G9.06c), len: 280 aa. Possible amidohydrolase, highly similar to NP_302587.1|NC_002677|CAC31966.1|AL583925 putative hydrolase from Mycobacterium leprae (271 aa). Also similar to other hydrolases and hypothetical proteins e.g. NP_601985.1|NC_003450 Predicted amidohydrolase from Corynebacterium glutamicum (266 aa); NP_459623.1|NC_003197 putative hydrolase from Salmonella typhimurium LT2 (262 aa); AL096822|SCGD3_8|NP_627996.1|NC_003888 probable hydrolase from Streptomyces coelicolor (264 aa), FASTA scores: opt: 368, E(): 6.1e-15, (34.2% identity in 272 aa overlap); YAUB_SCHPO|Q10166 hypothetical 35.7 kDa protein c26a3.11 from S. pombe (322 aa), FASTA scores: opt: 338, E():1.4e-13, (30.3% identity in 277 aa overlap); etc. Start changed since first submission (-60 aa). P9WJ01 No intermediary metabolism and respiration Q11146 P9WJ01 GO:0006807,GO:0016810 3.5.-.- P9WJ01 NC_000962.3 Mycobrowser_v5 CDS 570539 571648 . + 0 Rv0482 murB Involved in cell wall formation; peptidoglycan biosynthesis [catalytic activity: UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH]. Probable UDP-N-acetylenolpyruvoylglucosamine reductase MurB (UDP-N-acetylmuramate dehydrogenase) Rv0482, (MTCY20G9.08), len: 369 aa. Probable murB, UDP-N-acetylenolpyruvoylglucosamine reductase (see citation below), equivalent to CAC31964.1|AL583925 UDP-N-acetylenolpyruvoylglucosamine reductase from Mycobacterium leprae (367 aa). Also highly similar to others e.g. MURB_ECOLI|P08373 UDP-N-acetylenolpyruvoylglucosamine reductase from Escherichia coli (342 aa), FASTA scores: opt: 292, E(): 6.3e-12, (33.5% identity in 355 aa overlap); etc. Belongs to the MurB family. Cofactor: FAD. P9WJL9 No cell wall and cell processes P65460 P9WJL9 GO:0008762,GO:0007049,GO:0051301,GO:0007047,GO:0005737,GO:0055114,GO:0009252,GO:0008360,GO:0050660 1.1.1.158 P9WJL9 NC_000962.3 Mycobrowser_v5 CDS 575348 576790 . + 0 Rv0486 mshA Involved in the first step of mycothiol biosynthesis Glycosyltransferase MshA Rv0486, (MTCY20G9.12), len: 480 aa. MshA, glycosyltransferase (see citations below), highly similar to P54138|Y486_MYCLE|ML2443 possible glycosyl transferase from Mycobacterium leprae (428 aa); and S72892|B2168_C2_201 probable hexosyltransferase from Mycobacterium leprae (409 aa), FASTA scores: opt: 2375, E(): 0, (86.4% identity in 413 aa overlap). Also highly similar to CAC04040.1|AL391406 putative transferase from Streptomyces coelicolor (496 aa); and similar to various transferases e.g. NP_437172.1|NC_003078 putative membrane-anchored glycosyltransferase protein from Sinorhizobium meliloti (416 aa); O26550|U67601_1 LPS biosynthesis related protein from Methanococcus jannaschii (411 aa), FASTA score: (25.3% identity in 387 aa overlap); etc. Also similar to CAC87824.1|AJ316594 putative sucrose-phosphate synthase from Nostoc punctiforme (422 aa). Contains a match to Pfam entry PF00534 glycosyl_transf_1 - Glycosyl transferases group 1. P9WMY7 No intermediary metabolism and respiration P64707 P9WMY7 GO:0016757,GO:0009058 2.4.-.- P9WMY7 NC_000962.3 Mycobrowser_v5 CDS 577664 578269 . + 0 Rv0488 Rv0488 Unknown; possibly involved in transport of lysine across the membrane. Probable conserved integral membrane protein Rv0488, (MTCY20G9.14), len: 201 aa. Probable conserved integral membrane protein, LysE family possibly involved in transport of Lysine, similar to others and conserved hypothetical proteins e.g. AB93746.1|AL357613 putative membrane transport protein from Streptomyces coelicolor (204 aa); D83100|PA4365 probable transporter from Pseudomonas aeruginosa (200 aa); YGGA_ECOLI|P11667 hypothetical 21.7 kDa protein from Escherichia coli (197 aa), FASTA scores: opt: 382, E(): 1.1e-19, (39.1% identity in 179 aa overlap); CGLYSEG_2 C|P94633 lysine exporter protein (236 aa), FASTA scores: E(): 2.3e-07, (33.3% identity in 219 aa overlap). Also similar to Rv1986 from Mycobacterium tuberculosis. P9WK33 No cell wall and cell processes P64711 P9WK33 GO:0016021,GO:0005886,GO:0006865 NC_000962.3 Mycobrowser_v5 CDS 579349 580581 . + 0 Rv0490 senX3 Sensor part of a two component regulatory system. Probably forms part of a two-component regulatory system SENX3/REGX3; phosphorylates REGX3. Putative two component sensor histidine kinase SenX3 Rv0490, (MTCY20G9.16), len: 410 aa. Putative senX3, two-component sensor histidine kinase, transmembrane protein (see citations below), equivalent to O07129|SEX3_MYCBO sensor-like histidine kinase SENX3 from Mycobacterium bovis BCG (410 aa), FASTA scores: E(): 0, (99.5% identity in 410 aa overlap); and highly similar to P54883|SEX3_MYCLE|SENX3 sensor-like histidine kinase from Mycobacterium leprae (443 aa), FASTA score: (83.8% identity in 408 aa overlap). Also highly similar, except in N-terminus, to CAC31957.1|AL583925 probable two-component system sensor histidine kinase from Mycobacterium leprae (441 aa). Also highly similar to sensor kinase proteins from other organisms e.g. CAB77323.1|AL160331 putative sensor kinase protein from Streptomyces coelicolor (426 aa). P9WGK5 No regulatory proteins P0A600 P9WGK5 GO:0016021,GO:0018106,GO:0005886,GO:0007165,GO:0000155,GO:0000160,GO:0005524 2.7.13.3 P9WGK5 NC_000962.3 Mycobrowser_v5 CDS 581489 583378 . - 0 Rv0492c Rv0492c Function unknown; probably involved in cellular metabolism. Probable oxidoreductase GMC-type Rv0492c, (MT0511/MT0512, MTCY20G9.18c), len: 629 aa. Probable oxidoreductase GMC type, similar to others except in N-terminus e.g. P55582|AE000087_5|Y4NJ_RHISN hypothetical GMC-type oxidoreductase from Rhizobium sp. (505 aa), FASTA scores: opt: 873, E():0, (34.3% identity in 502 aa overlap); YTH2_RHOER|P46371 hypothetical 53.0 kDa GMC-type oxidoreductase from Rhodococcus erythropolis (493 aa), FASTA score: (25.7% identity in 521 aa overlap); YTH2_RHOSO|P46371 hypothetical 53.0 kDa gmc-type oxidoreductase from Rhodococcus erythropolis (493 aa), FASTA score: (25.7% identity in 521 aa overlap); NP_085596.1|NC_002679 probable oxidoreductase from Mesorhizobium loti (507 aa); NP_285451.1|NC_001264 GMC oxidoreductase from Deinococcus radiodurans (722 aa); NP_249055.1|NC_002516 probable oxidoreductase from Pseudomonas aeruginosa (531 aa); etc. Contains PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature, and PS00624 GMC oxidoreductases signature 2. Belongs to the GMC oxidoreductases family. Cofactor: FAD (by similarity). Note that start changed since first submission (previously 684 aa). P9WMV7 No intermediary metabolism and respiration Q11157 P9WMV7 GO:0055114,GO:0016614,GO:0050660 1.1.-.- P9WMV7 NC_000962.3 Mycobrowser_v5 CDS 591111 591347 . + 0 Rv0500A Rv0500A Function unknown Conserved protein Rv0500A, len: 78 aa. Conserved protein, similar to proteins from Mycobacterium leprae and Streptomyces coelicolor e.g. U00018_25 from Mycobacterium leprae cosmid B2168 (86 aa), FASTA scores: opt: 428, E(): 1.3e-27, (82.6% identity in 86 aa overlap); AL079345|SCE68_26 from Streptomyces coelicolor cosmid E6 (70 aa), FASTA scores: opt: 252, E(): 1.2 e-13, (72.2 identity in 54 aa overlap). P9WKT7 No conserved hypotheticals P9WKT7 GO:0003677 NC_000962.3 Mycobrowser_v5 CDS 591654 592784 . + 0 Rv0501 galE2 Involved in galactose metabolism [catalytic activity: UDP-glucose = UDP-galactose]. Possible UDP-glucose 4-epimerase GalE2 (galactowaldenase) (UDP-galactose 4-epimerase) (uridine diphosphate galactose 4-epimerase) (uridine diphospho-galactose 4-epimerase) Rv0501, (MTCY20G9.28), len: 376 aa. Possible galE2, UDP-glucose 4-epimerase, highly similar (except in N-terminus) to CAC31944.1|AL583925 possible glucose epimerase/dehydratase from Mycobacterium leprae (364 aa). N-terminus highly similar to S72923|B2168_C1_174|467075|AAA17259.1|U00018 hypothetical protein from Mycobacterium leprae (180 aa), FASTA scores: opt: 934, E(): 0, (89.6% identity in 164 aa overlap); and C-terminus highly similar to S72898|467050|AAA17234.1|U00018 hypothetical protein from Mycobacterium leprae (168 aa), FASTA scores: opt: 928, E(): 0, (82.7% identity in 168 aa overlap). Also highly similar to T36274|5123671|CAB45360.1|AL079345 probable epimerase from Streptomyces coelicolor (353 aa); and similar in part to other epimerases e.g. GALE_ECOLI|P09147 UDP-glucose 4-epimerase from Escherichia coli (338 aa), FASTA scores: opt: 241, E(): 6.7e-09, (28.2% identity in 294 aa overlap); etc. Belongs to the sugar epimerase family. Cofactor: NAD. Note that previously known as galE1. P9WKT3 No intermediary metabolism and respiration P0A5D1 P9WKT3 GO:0044237,GO:0050662,GO:0003824 P9WKT3 NC_000962.3 Mycobrowser_v5 CDS 593871 594779 . - 0 Rv0503c cmaA2 Essential for the cyclopropanation function. Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge. Mycolic acids, which represent the major constituent of mycobacterial cell wall complex, act as substrates [catalytic activity: S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid]. Cyclopropane-fatty-acyl-phospholipid synthase 2 CmaA2 (cyclopropane fatty acid synthase) (CFA synthase) (cyclopropane mycolic acid synthase 2) (mycolic acid trans-cyclopropane synthetase) Rv0503c, (MTCY20G9.30c), len: 302 aa. CmaA2 (alternate gene name: cma2), cyclopropane-fatty-acyl-phospholipid synthase 2 (mycolic acid trans-cyclopropane synthetase) (see citations below). Note that this protein has 302 aa and not 322 aa: we have chosen a different initiation codon on the basis of homology. Equivalent to S72886|B2168_F3_130 hypothetical protein from Mycobacterium leprae (308 aa), FASTA score: (78.9% identity in 303 aa overlap); and highly similar to other proteins from Mycobacterium leprae. Also similar to other proteins from Mycobacterium tuberculosis and Mycobacterium bovis BCG e.g. MTV038_14|UMAA2|Rv0470c|MTV038.14 putative mycolic acid synthesis/modification protein (287 aa) (57.2% identity in 297 aa overlap). P9WPB5 No lipid metabolism 1KPI,3HEM P0A5P0 P9WPB5 GO:0005737,GO:0008610,GO:0008825 2.1.1.79 P9WPB5 NC_000962.3 Mycobrowser_v5 CDS 595464 596585 . - 0 Rv0505c serB1 Removes a phosphate from phosphoserine [catalytic activity: phosphoserine + H2O = serine + phosphate]. Possible phosphoserine phosphatase SerB1 (PSP) (O-phosphoserine phosphohydrolase) (pspase) Rv0505c, (MTCY20G9.32c), len: 373 aa. Possible serB1, phosphoserine phosphatase, equivalent (but longer ~70 aa in N-terminus) to S72914|serB phosphoserine phosphatase from Mycobacterium leprae (300 aa), FASTA scores: opt: 1570, E(): 0, (83.0% identity in 306 aa overlap). C-terminus highly similar to CAB55344.1|AJ010584 phosphoserine phosphatase from Streptomyces coelicolor (266 aa). Low similarity to SERB_ECOLI|P06862 phosphoserine phosphatase from Escherichia coli strains K12 and O157:H7 (322 aa), FASTA scores: opt: 148, E(): 0.043, (24.0% identity in 150 aa overlap). C-terminus is also similar to O33611|AB004855_1|IMD_STRCN protein involved in inhibition of morphological differentiation from Streptomyces cyaneus (277 aa), FASTA score: (37.7% identity in 252 aa overlap). Seems to belong to the SERB family. Note that previously known as serB. P9WGJ3 No intermediary metabolism and respiration P66801 P9WGJ3 GO:0016791,GO:0008152 P9WGJ3 NC_000962.3 Mycobrowser_v5 CDS 613038 614342 . + 0 Rv0522 gabP Involved in 4-aminobutyrate (GABA) degradation pathway. Transporter for GABA. Responsible for the translocation of the substrate across the membrane. Probable GABA permease GabP (4-amino butyrate transport carrier) (GAMA-aminobutyrate permease) Rv0522, (MTCY20G10.12), len: 434 aa. Probable gabP, GABA permease (gamma-aminobutyrate permease), integral membrane protein, highly similar to others e.g. GABP_ECOLI|P25527 gaba permease from Escherichia coli (466 aa), FASTA scores: opt: 1218, E(): 0, (44.3% identity in 424 aa overlap); etc. Also similar to other M. tuberculosis permeases e.g. MTCY13E10.06c FASTA score: (34.4% identity in 407 aa overlap). Contains PS00218 Amino acid permeases signature. Overlaps and extends Rv0523c|MTCY25D10.01 from overlapping cosmid. Belongs to the amino acid permease family (APC family). L7N6B9 No cell wall and cell processes Q8VKK1 L7N6B9 GO:0006865,GO:0016021,GO:0055085,GO:0015171 L7N6B9 NC_000962.3 Mycobrowser_v5 CDS 3004035 3004748 . - 0 Rv2687c Rv2687c Thought to be involved in active transport of unidentified antibiotic across the membrane (export): antibiotic resistance by an export mechanism. Responsible for the translocation of the substrate across the membrane. Antibiotic-transport integral membrane leucine and valine rich protein ABC transporter Rv2687c, (MTCY05A6.08c), len: 237 aa. Antibiotic-transport integral membrane leu-, val-rich protein ABC transporter (see citation below), showing some similarity with two other hypothetical proteins, BAB59668|TVG0517148 from Thermoplasma volcanium (241 aa), FASTA scores: opt: 136, E(): 0.32, (23.1% identity in 208 aa overlap); and Q97U55|SSO3168 from Sulfolobus solfataricus (249 aa), FASTA scores: opt: 136, E(): 0.33, (25.15% identity in 195 aa overlap). Has some hydrophobic stretches and contains bacterial regulatory proteins, araC family signature (PS00041). P9WJB1 No cell wall and cell processes P9WJB1 GO:0005886,GO:0046677,GO:0006810,GO:0016021 P9WJB1 NC_000962.3 Mycobrowser_v5 CDS 601857 602786 . + 0 Rv0510 hemC Involved in porphyrin biosynthesis by the C5 pathway (at the fourth step). Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane preuroporphyrinogen in several discrete steps [catalytic activity: 4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH3]. Probable porphobilinogen deaminase HemC (PBG) (hydroxymethylbilane synthase) (HMBS) (pre-uroporphyrinogen synthase) Rv0510, (MTCY21C8.01-MTCY20G9.37), len: 309 aa. Probable hemC, hydroxymethylbilane synthase (porphobilinogen deaminase), equivalent to HEM3B|Q49808|HEM3_MYCLE porphobilinogen deaminase from Mycobacterium leprae (315 aa), FASTA scores: opt: 889, E(): 0, (88.1% identity in 159 aa overlap). Also highly similar to others e.g. Q9WX16|HE31_STRCO probable porphobilinogen deaminase from Streptomyces coelicolor (319 aa); Q9L6Q2|HEM3_SALTY porphobilinogen deaminase from Salmonella typhimurium (313 aa); etc. Belongs to the HMBS family. Cofactor: covalently binds a dipyrromethane cofactor to which the porphobilinogen subunits are ADDED. P9WMP3 No intermediary metabolism and respiration P64336 P9WMP3 GO:0018160,GO:0006779,GO:0004418 2.5.1.61 P9WMP3 NC_000962.3 Mycobrowser_v5 CDS 602819 604516 . + 0 Rv0511 hemD Possibly involved in the biosynthesis of siroheme and cobalamin [catalytic activity: 2 S-adenosyl-L-methionine + uroporphyrin III = 2 S-adenosyl-L-homocysteine + sirohydrochlorin]. Probable uroporphyrin-III C-methyltransferase HemD (uroporphyrinogen III methylase) (urogen III methylase) (SUMT) (urogen III methylase) (UROM) Rv0511, (MTCY21C8.02), len: 565 aa. Probable hemD (alternate gene name: cysG), uroporphyrin-III C-methyltransferase, highly similar to others e.g. CAC31936.1|AL583925 possible uroporphyrin-III C-methyltransferase from Mycobacterium leprae (563 aa); and S72909|CYSG from Mycobacterium leprae (472 aa), FASTA scores: opt: 1946, E(): 0, (83.3% identity in 472 aa overlap); T36265|5123662|CAB45351.1|AL079345 probable uroporphyrin-III C-methyltransferase from Streptomyces coelicolor (565 aa); and similar to others e.g. AAK00606.1|AF221100_3|AF221100 from Selenomonas ruminantium subsp. ruminantium (505 aa); etc. Also similar to Rv2071c and Rv2847c from Mycobacterium tuberculosis. Note that previously known as cysG. Q6MX34 No intermediary metabolism and respiration Q6MX34 Q6MX34 GO:0004851,GO:0004852,GO:0033014 2.1.1.107 Q6MX34 NC_000962.3 Mycobrowser_v5 CDS 614835 616223 . + 0 Rv0524 hemL Involved in porphyrin biosynthesis by the C5 pathway (at the second step) [catalytic activity: (S)-4-amino-5-oxopentanoate = 5-aminolevulinate]. Probable glutamate-1-semialdehyde 2,1-aminomutase HemL (GSA) (glutamate-1-semialdehyde aminotransferase) (GSA-at) Rv0524, (MTCY25D10.03), len: 462 aa. Probable hemL, glutamate-1-semialdehyde 2,1-aminomutase, equivalent to P46716|GSA_MYCLE glutamate-1-semialdehyde 2,1-aminomutase from Mycobacterium leprae (446 aa), FASTA scores: opt: 1532, E(): 0, (82.6% identity in 460 aa overlap). Also highly similar to others e.g. Q9F2S0|GSA_STRCO from Streptomyces coelicolor (438 aa); Q06774|GSA_PROFR from Propionibacterium freudenreichii (441 aa); etc. Contains PS00600 Aminotransferases class-III pyridoxal-phosphate attachment site. Belongs to class-III of pyridoxal-phosphate-dependent aminotransferases. Cofactor: pyridoxal phosphate. P9WMN9 No intermediary metabolism and respiration P63506 P9WMN9 GO:0042286,GO:0006779,GO:0030170,GO:0008483,GO:0005737 5.4.3.8 P9WMN9 NC_000962.3 Mycobrowser_v5 CDS 617493 618272 . + 0 Rv0527 ccdA Possibly involved in cytochrome C synthesis. Might transfer reducing equivalents across the cytoplasmic membrane, promoting efficient disulfide bond isomerization of proteins localized on the outer surface of the membrane. Possible cytochrome C-type biogenesis protein CcdA Rv0527, (MTCY25D10.06), len: 259 aa. Possible ccdA, cytochrome C-type biogenesis protein, integral membrane protein, equivalent to Q49810|B2168_C1_192|S72890 hypothetical protein from Mycobacterium leprae (262 aa), FASTA scores: opt: 1341, E(): 0, (79.0% identity in 262 aa overlap). Also highly similar to others e.g. CAC08380.1 (253 aa); CCDA_BACSU|P45706 cytochrome C-type biogenesis protein from Bacillus subtilis (235 aa), FASTA scores: opt: 307, E(): 7.4e-13, (30.4% identity in 237 aa overlap); etc. Seems to belong to the DSBD subfamily. Note that previously known as ccsA. L7N671 No intermediary metabolism and respiration Q7D9P9 L7N671 GO:0016020,GO:0055114,GO:0017004 NC_000962.3 Mycobrowser_v5 CDS 622329 622646 . + 0 Rv0531 Rv0531 Unknown Possible conserved membrane protein Rv0531, (MTCY25D10.10), len: 105 aa. Possible conserved membrane protein, highly similar to Y13803|MLB1306_1|CAA74131.1 hypothetical protein from Mycobacterium leprae (86 aa), FASTA scores: E(): 2.1e-24, (74.4% identity in 86 aa overlap); and NP_302557.1|NC_002677 putative membrane protein from Mycobacterium leprae (111 aa). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06397 No cell wall and cell processes O06397 NC_000962.3 Mycobrowser_v5 CDS 624473 625480 . - 0 Rv0533c fabH Involved in fatty acid biosynthesis. Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. KAS III catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities [catalytic activity: acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]]. 3-oxoacyl-[acyl-carrier-protein] synthase III FabH (beta-ketoacyl-ACP synthase III) (KAS III) Rv0533c, (MTCY25D10.12c), len: 335 aa. FabH (alternate gene name: mtFabH), 3-oxoacyl-[acyl-carrier protein] synthase III (see citations below), highly similar to others e.g. Q54206|FABH from streptomyces glaucescens (333 aa), FASTA scores: opt: 1109, E(): 0, (51.4% identity in 333 aa overlap); FABH_ECOLI|P24249 3-oxoacyl-[acyl-carrier-protein] synthase III (317 aa), FASTA scores: opt: 666, E(): 0, (37.1% identity in 318 aa overlap); etc. Belongs to the FabH family. P9WNG3 No lipid metabolism 1HZP,1M1M,1U6E,1U6S,2AHB,2AJ9,2QNX,2QNY,2QNZ,2QO0,2QO1,2QX1 P0A574 P9WNG3 GO:0033818,GO:0005737,GO:0006633,GO:0004315 2.3.1.180 P9WNG3 NC_000962.3 Mycobrowser_v5 CDS 626457 627251 . + 0 Rv0535 pnp Phosphorylates 5'-methylthioadenosin [catalytic activity: 5'-methylthioadenosine + phosphate = adenine + 5-methylthio-D-ribose 1-phosphate]. Probable 5'-methylthioadenosine phosphorylase Pnp (MTA phosphorylase) Rv0535, (MTCY25D10.14c), len: 264 aa. Probable pnp, 5'-methylthioadenosine phosphorylase, highly similar to others e.g. CAB90972.1|AL355832 putative methylthioadenosine phosphorylase from Streptomyces coelicolor (280 aa); etc. Also similar to Rv3307|deoD probable purine nucleoside phosphorylase from Mycobacterium tuberculosis (268 aa). Belongs to the PNP/MTAP family 2 of phosphorylases. Gene name could be inappropriate. O06401 No intermediary metabolism and respiration O06401 O06401 GO:0009116,GO:0017061 2.4.2.28 O06401 NC_000962.3 Mycobrowser_v5 CDS 627248 628288 . + 0 Rv0536 galE3 Possibly involved in galactose metabolism [catalytic activity: UDP-glucose = UDP-galactose]. Probable UDP-glucose 4-epimerase GalE3 (galactowaldenase) (UDP-galactose 4-epimerase) (uridine diphosphate galactose 4-epimerase) (uridine diphospho-galactose 4-epimerase) Rv0536, (MTCY25D10.15), len: 346 aa. Possible galE3, UDP-glucose 4-epimerase, highly similar to CAB76986.1|AL159178 putative epimerase from Streptomyces coelicolor (334 aa); and similar to other epimerases e.g. NP_436775.1|NC_003078 putative NDP-glucose dehydrataseepimerase protein from Sinorhizobium meliloti (368 aa); AF143772|AF143772_7 GepiA from Mycobacterium avium strain 2151 (353 aa), FASTA scores: opt: 577, E(): 3.9e-29, (36.6% identity in 352 aa overlap); GALE_METJA|Q57664 putative UDP-glucose 4-epimerase (305 aa), FASTA scores: opt: 300, E(): 1.6e-12, (30.9% identity in 343 aa overlap); etc. Also similar to Mycobacterium tuberculosis proteins e.g. Rv3634c, Rv3784, etc. Seems to belong to the sugar epimerase family. Note that previously known as galE2. L7N670 No intermediary metabolism and respiration Q7D9P4 L7N670 GO:0005975,GO:0044237,GO:0050662,GO:0003978 5.1.3.2 L7N670 NC_000962.3 Mycobrowser_v5 CDS 634416 635504 . - 0 Rv0542c menE Involved in menaquinone biosynthesis. O-succinylbenzoic acid (OSB) to O-succinylbenzoyl-CoA (OSB-CoA) [catalytic activity: ATP + O-succinylbenzoate + CoA = AMP + diphosphate + O-succinylbenzoyl-CoA]. Possible O-succinylbenzoic acid--CoA ligase MenE (OSB-CoA synthetase) (O-succinylbenzoate-CoA synthase) Rv0542c, (MTCY25D10.21c), len: 362 aa. Possible menE, O-succinylbenzoic acid-CoA ligase, highly similar to Q50170|AAA63145.1|U15187|XCLB 4-Coumarate--CoA ligase from Mycobacterium leprae (352 aa), FASTA scores: opt: 1815, E(): 0, (78.9% identity in 351 aa overlap). Also similar to N-terminus of acid-CoA ligases e.g. NP_471116.1|NC_003212 O-succinylbenzoic acid-CoA ligase from Listeria innocua (469 aa); NP_390957.1|NC_000964 O-succinylbenzoic acid-CoA ligase from Bacillus subtilis (486 aa); MENE_HAEIN|P44565 O-succinylbenzoic acid-CoA ligase from Haemophilus influenzae (452 aa), FASTA scores: opt: 307, E(): 4.6e-12, (25.4% identity in 339 aa overlap); etc. Also some similarity with fadD proteins from Mycobacterium tuberculosis. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family. P9WQ39 No intermediary metabolism and respiration O06408 P9WQ39 GO:0008756,GO:0008152 6.2.1.26 P9WQ39 NC_000962.3 Mycobrowser_v5 CDS 635935 636213 . - 0 Rv0544c Rv0544c Unknown Possible conserved transmembrane protein Rv0544c, (MTCY25D10.23c), len: 92 aa. Possible conserved transmembrane protein, equivalent to NP_302470.1|NC_002677 possible membrane protein from Mycobacterium leprae (96 aa); and shows some similarity to MLU15187_33|Q50172|U296V from Mycobacterium leprae (36 aa), FASTA scores: opt: 151, E(): 2.1e-05, (71.4% identity in 35 aa overlap). Also some similarity with VATL_NEPNO|Q26250 vacuolar ATP synthase 16 kDa proteolipid from Nephrops norvegicus (159 aa), FASTA scores: opt: 80, E(): 11, (26.1% identity in 88 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06410 No cell wall and cell processes O06410 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 636210 637463 . - 0 Rv0545c pitA Involved in low-affinity inorganic phosphate transport across the membrane. Responsible for the translocation of the substrate across the membrane. Probable low-affinity inorganic phosphate transporter integral membrane protein PitA Rv0545c, (MTCY25D10.24c), len: 417 aa. Probable pitA, low-affinity inorganic phosphate transporter, integral membrane protein, equivalent to Q50173|NP_302471.1 pitA from Mycobacterium leprae (414 aa), FASTA scores: opt: 2035, E(): 0, (76.3% identity in 418 aa overlap). Also highly similar to others e.g. CAB59461.1|AL132644 putative low-affinity phosphate transport protein from Streptomyces coelicolor (423 aa); PITA_ECOLI|P37308 low-affinity inorganic phosphate transporter from Escherichia coli (499 aa), FASTA scores: opt: 304, E(): 6.9e-10, (32.5 % identity in 234 aa overlap); etc. Belongs to the PHO-4 family of transporters, pit subfamily. P9WIA7 No cell wall and cell processes O06411 P9WIA7 GO:0016021,GO:0006817,GO:0005886,GO:0005315 P9WIA7 NC_000962.3 Mycobrowser_v5 CDS 641096 642811 . - 0 Rv0551c fadD8 Function unknown, but involved in lipid degradation. Probable fatty-acid-CoA ligase FadD8 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv0551c, (MTCY25D10.30c), len: 571 aa. Probable fadD8, fatty-acid-CoA synthetase, similar to many e.g. LCFA_ECOLI|P29212 long-chain-fatty-acid--CoA ligase (561 aa), FASTA scores: opt: 585, E(): 9.5e-30, (28.7% identity in 536 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Note other possible start sites exist downstream of this start. O06417 No lipid metabolism O06417 O06417 GO:0008152,GO:0016874 6.2.1.- O06417 NC_000962.3 Mycobrowser_v5 CDS 688032 688859 . + 0 Rv0590 mce2B Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce2B Rv0590, (MTCY19H5.32c), len: 275 aa. Mce2B; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07414|Rv0170|MTCI28.10|mce1B (346 aa); O53968|Rv1967|MTV051.05|mce3B (342 aa); etc. Also highly similar to others e.g. NP_302657.1|NC_002677 putative secreted protein from Mycobacterium leprae (346 aa); P45391|YRBD_ECOLI hypothetical 19.6 kDa protein from Escherichia coli (183 aa), FASTA scores: opt: 160, E(): 0.00099, (28.3% identity in 166 aa overlap); P45029|YRBD_HAEIN|HI1085 hypothetical protein from Haemophilus influenzae (167 aa), FASTA scores: opt: 135, E():0.035, (25.9% identity in 143 aa overlap); etc. Contains possible N-terminal signal or anchor sequence. Predicted to be an outer membrane protein (See Song et al., 2008). O07788 No virulence, detoxification, adaptation O07788 O07788 O07788 NC_000962.3 Mycobrowser_v5 CDS 646298 647962 . + 0 Rv0555 menD Involved in menaquinone biosynthesis (at the first step) [catalytic activity 1: isochorismate + 2-ketoglutarate = 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate + pyruvate + CO(2)] [catalytic activity 2: 2-oxoglutarate = succinate semialdehyde + CO(2)]. Probable bifunctional menaquinone biosynthesis protein MenD : 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (SHCHC synthase) + 2-oxoglutarate decarboxylase (alpha- ketoglutarate decarboxylase) (KDC) Rv0555, (MTCY25D10.34), len: 554 aa. Probable menD, menaquinone biosynthesis protein, including 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase and 2-oxoglutarate decarboxylase activities. Equivalent to NP_302478.1|NC_002677 putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase / 2-oxoglutarate decarboxylase from Mycobacterium leprae (556 aa). Also similar to others e.g. MEND_BACSU|P23970 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase from Bacillus subtilis (548 aa), FASTA scores: opt: 488, E(): 2.3e-21, (34.3% identity in 545 aa overlap); etc. Cofactor: thiamine pyrophosphate. P9WK11 No intermediary metabolism and respiration O06421 P9WK11 GO:0000287,GO:0030145,GO:0009234,GO:0030976,GO:0070204 2.2.1.9 P9WK11 NC_000962.3 Mycobrowser_v5 CDS 649689 650393 . + 0 Rv0558 menH Involved in menaquinone biosynthesis (at the last step) converts DMKH2 into MKH2 [catalytic activity: S-adenosyl-L-methionine + demethylmenaquinol = S-adenosyl-L-homocysteine + menaquinol]. Probable ubiquinone/menaquinone biosynthesis methyltransferase MenH (2-heptaprenyl-1,4-naphthoquinone methyltransferase) Rv0558, (MTCY25D10.37), len: 234 aa. Probable menH (alternate gene name: menG), ubiquinone/menaquinone biosynthesis methlytransferase (2-heptaprenyl-1,4-naphthoquinone methyltransferase), equivalent to NP_302480.1|NC_002677 putative ubiquinone/menaquinone biosynthesis methyltransferase from Mycobacterium leprae (238 aa). Also highly similar to others e.g. CAB44537.1|AL078618|T34630 from Streptomyces coelicolor (231 aa); UBIE_ECOLI|P27851 from Escherichia coli strain K12 (251 aa), FASTA scores: opt: 421, E(): 1.2e-21, (43.2% identity in 227 aa overlap); GRC2_BACSU|P31113 from Bacillus subtilis (233 aa), FASTA scores: opt: 345, E(): 1.4e-16, (34.6% identity in 231 aa overlap); etc. Belongs to the UbiE family. Note that previously known as ubiE. P9WFR3 No intermediary metabolism and respiration P0A638 P9WFR3 GO:0008168,GO:0009234 2.1.1.- P9WFR3 NC_000962.3 Mycobrowser_v5 CDS 650407 650745 . - 0 Rv0559c Rv0559c Unknown Possible conserved secreted protein Rv0559c, (MTCY25D10.38c), len: 112 aa. Possible conserved secreted protein, similar to NP_302481.1|NC_002677 putative secreted protein from Mycobacterium leprae (112 aa). Also similar to Y08B_MYCTU|Q11048 hypothetical 11.6 kDa protein FASTA scores: opt: 111, E(): 011, (25.4% identity in 114 aa overlap). Contains possible N-terminal signal sequence. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WKL3 No cell wall and cell processes O06425 P9WKL3 P9WKL3 NC_000962.3 Mycobrowser_v5 CDS 653879 654739 . + 0 Rv0563 htpX Possibly involved in adaptation. Hydrolizes specific peptides and/or proteins. Probable protease transmembrane protein heat shock protein HtpX Rv0563, (MTV039.01, MTCY25D10.42), len: 286 aa. (alternative start at position 654006). Probable htpX, protease heat shock protein X (transmembrane protein), equivalent to NP_302484.1|NC_002677 putative peptidase from Mycobacterium leprae (287 aa). Also highly similar to others e.g. CAC08262.1|AL392146 putative peptidase from Streptomyces coelicolor (287 aa); NP_387431.1|NC_003047 putative protease transmembrane protein from Sinorhizobium meliloti (319 aa); NP_105051.1|NC_002678 heat shock protein (htpX) from Mesorhizobium loti (336 aa); NP_248692.1|NC_000909|U67608|MJU67608_8 heat shock protein HtpX, possibly protease (htpX) from Methanococcus jannaschii (284 aa), FASTA scores: opt: 660, E(): 0, (46.5 identity in 245 aa overlap). Continuation of MTCY25D10.42. Belongs to peptidase family M48 (zinc metalloprotease). Cofactor: Zinc. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P9WHS5 No virulence, detoxification, adaptation P65815 P9WHS5 GO:0004222,GO:0005886,GO:0006508,GO:0008270,GO:0016021 3.4.24.- NC_000962.3 Mycobrowser_v5 CDS 1265472 1266488 . - 0 Rv1138c Rv1138c Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv1138c, (MTCI65.05c), len: 338 aa. Possible oxidoreductase, similar to Q9EWQ8 putative oxidoreductase from Streptomyces coelicolor (343 aa). Also similar to many Mycobacterium tuberculosis hypothetical proteins e.g. Rv1751|P72008|MTCY04C12.35 (412 aa), fasta scores: opt: 89, E(): 4.5e-09, (24.6% identity in 358 aa overlap). O06538 No intermediary metabolism and respiration O06538 O06538 GO:0004497,GO:0055114,GO:0009055 1.-.-.- O06538 NC_000962.3 Mycobrowser_v5 CDS 659450 660868 . + 0 Rv0568 cyp135B1 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Possible cytochrome P450 135B1 Cyp135B1 Rv0568, (MT0594, MTV039.06), len: 472 aa. Possible cyp135B1, cytochrome P450, similar to putative cytochrome P-450 monoxygenases and other cytochrome P-450 related enzymes e.g. P29980|CPXN_ANASP probable cytochrome P450 from Anabaena sp. strain PCC 7120 (459 aa), FASTA scores: opt: 525, E(): 7.2e-27, (31.9% identity in 417 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. Rv0327c|NP_214841.1|NC_000962|CYP135A1|MT0342|MTCY63.32c putative cytochrome P450 (449 aa), FASTA scores: opt: 1080, E(): 0, (40.5% identity in 444 aa overlap); Rv3685c|NP_218202.1|NC_000962 putative cytochrome P450 (476 aa); Rv0136|NP_214650.1|NC_000962 putative cytochrome P450 (441 aa); etc. Contains cytochrome P450 cysteine heme-iron ligand signature (PS00086). P9WPM9 No intermediary metabolism and respiration P63715 P9WPM9 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPM9 NC_000962.3 Mycobrowser_v5 CDS 661295 663373 . + 0 Rv0570 nrdZ Involved in the DNA replication pathway (at the first reaction). Provides the precursors necessary for DNA synthesis [catalytic activity: 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O = ribonucleoside diphosphate + reduced thioredoxin]. Probable ribonucleoside-diphosphate reductase (large subunit) NrdZ (ribonucleotide reductase) Rv0570, (MTV039.08), len: 692 aa. Probable nrdZ, ribonucleoside-diphosphate reductase, large subunit, highly similar to others e.g. NP_070492.1|NC_000917|NRD|AE000988_11 ribonucleotide reductase from Archaeoglobus fulgidus (752 aa), FASTA scores: opt: 2001, E(): 0, (52.5% identity in 562 aa overlap) (N-terminus shorter); U73619|TAU73619_1|T37459 ribonucleotide reductase from Thermoplasma acidophilum (857 aa), FASTA scores: opt: 1678, E(): 0, (43.7% identity in 723 aa overlap); etc. Belongs to the ribonucleoside diphosphate reductase large chain family. P9WH77 No information pathways O53767 P9WH77 GO:0006260,GO:0016960,GO:0031419,GO:0055114,GO:0005971,GO:0005524 1.17.4.1 P9WH77 NC_000962.3 Mycobrowser_v5 CDS 665851 667242 . - 0 Rv0573c pncB2 Involved in NAD salvage. Phosphoribosylation of nicotinic acid. [catalytic activity: nicotinate + 5-phosphoribosyl 1-pyrophosphate = nicotinate mononucleotide + diphosphate] Nicotinic acid phosphoribosyltransferase PncB2 Rv0573c, (MTV039.11c), len: 463 aa. PncB2, nicotinic acid phosphoribosyltransferase (See Boshoff et al., 2008). Similar to e.g. NP_213718.1|NC_000918 hypothetical protein from Aquifex aeolicus (426 aa); AL109962|T36953|SCJ1.20 conserved hypothetical protein from Streptomyces coelicolor (438 aa), FASTA scores: opt: 1089, E(): 0, (49.4% identity in 385 aa overlap); P_391053.1|Z99120|BSUB0017_57|NC_000964 protein similar to nicotinate phosphoribosyltransferase from Bacillus subtilis (490 aa), FASTA scores: opt: 955, E():0, (43.5% identity in 356 aa overlap); etc. Also similar to Q10641|Y03F_MYCTU|MTCY130.15c|Rv1330c conserved hypothetical protein from Mycobacterium tuberculosis (509 aa), FASTA scores: opt: 761, E(): 0, (38.4% identity in 437 aa overlap). P9WJI7 No intermediary metabolism and respiration O53770 P9WJI7 GO:0019363,GO:0004516 2.4.2.11 P9WJI7 NC_000962.3 Mycobrowser_v5 CDS 838451 840856 . + 0 Rv0747 PE_PGRS10 Function unknown PE-PGRS family protein PE_PGRS10 Rv0747, (MTV041.21), len: 801 aa. PE_PGRS10, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to part of MTCY28.25c|Rv1759c|Z95890 antigen wag22 from M. tuberculosis (914 aa), FASTA scores: opt: 2772, E(): 0, (60.9% identity in 941 aa overlap). Also similar to other PE-PGRS family proteins e.g. Z95844|MTCY493_2 FASTA score: (50.2% identity in 815 aa overlap). Contains PS00012 Phosphopantetheine attachment site. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WIG1 No PE/PPE O53810 P9WIG1 P9WIG1 NC_000962.3 Mycobrowser_v5 CDS 671996 675916 . - 0 Rv0578c PE_PGRS7 Function unknown PE-PGRS family protein PE_PGRS7 Rv0578c, (MTV039.16c), len: 1306 aa. PE_PGRS7, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to many other PGRS proteins e.g. MTCY493.04|Z95844 from Mycobacterium tuberculosis (1329 aa), FASTA scores: opt: 3994, E(): 0, (54.6% identity in 1375 aa overlap). Contains two PS00583 pfkB family of carbohydrate kinases signatures possibly fortuitously. Q6MX28 No PE/PPE Q6MX28 Q6MX28 Q6MX28 NC_000962.3 Mycobrowser_v5 CDS 678389 679075 . - 0 Rv0583c lpqN Unknown Probable conserved lipoprotein LpqN Rv0583c, (MTV039.21c), len: 228 aa. Probable lpqN, conserved lipoprotein, equivalent to AAA90989.1|U20446|MK35|U20446|MKU20446_1 lipoprotein precursor from Mycobacterium kansasii (225 aa), FASTA scores: opt: 945, E(): 0, (62.7% identity in 228 aa overlap); and similar to others from Mycobacteria e.g. Rv0040c and Rv1016c from Mycobacterium tuberculosis. Contains N-terminal signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O53780 No cell wall and cell processes O53780 O53780 O53780 NC_000962.3 Mycobrowser_v5 CDS 681885 684272 . - 0 Rv0585c Rv0585c Unknown Probable conserved integral membrane protein Rv0585c, (MTV039.23c, MTCY19H5.37), len: 795 aa. Probable conserved integral membrane protein. C-terminus similar to CAB88984.1|AL353864 putative integral membrane protein from Streptomyces coelicolor (299 aa); and C-terminal region of CAC01311.1|AL390968 putative integral membrane protein from Streptomyces coelicolor (925 aa). Also some similarity with Rv0204 from Mycobacterium tuberculosis. O53781 No cell wall and cell processes O53781 O53781 NC_000962.3 Mycobrowser_v5 CDS 685928 686815 . + 0 Rv0588 yrbE2B Unknown Conserved hypothetical integral membrane protein YrbE2B Rv0588, (MTCY19H5.34c), len: 295 aa. YrbE2B, hypothetical unknown integral membrane protein, part of mce2 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07413|Rv0168|MTCI28.08|yrbE1B (289 aa); O53966|Rv1965|MTV051.03|yrbE3B (271 aa); etc. Also highly similar to conserved hypothetical integral membrane proteins of the yrbEB type, e.g. P45392|YRBE_ECOLI hypothetical 27.9 kDa protein from Escherichia coli (260 aa), FASTA scores: opt: 232, E(): 8.4e-08, (22.1 % identity in 267 aa overlap); P45030|YRBE_HAEIN|HI1086 hypothetical protein from Haemophilus influenzae (261 aa), FASTA scores: opt: 234, E(): 6.3e-08, (24.2% identity in 215 aa overlap); NP_302655.1|NC_002677 conserved membrane protein from Mycobacterium leprae (289 aa); etc. O07790 No virulence, detoxification, adaptation O07790 O07790 NC_000962.3 Mycobrowser_v5 CDS 686821 688035 . + 0 Rv0589 mce2A Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce2A Rv0589, (MTCY19H5.33c), len: 404 aa. Mce2A; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins P72013|MCE1|Rv0169|MTCI28.09|mce1A (454 aa); O53967|MCE3|Rv1966|MTV051.04|mce3A (425 aa); etc. Also highly similar to others e.g. AAD52105.1|AF113402_1|AF113402 mycobacterial cell entry protein from Mycobacterium bovis BCG (454 aa); NP_302656.1|NC_002677 putative cell invasion protein from Mycobacterium leprae (441 aa); CAC12798.1|AL445327 putative secreted protein from Streptomyces coelicolor (418 aa); etc. Also highly similar, but longer 21 aa, to P72013|CAA50257.1|X70901|MTCI28.08 Mcep protein from Mycobacterium tuberculosis (432 aa), FASTA scores: opt: 1324, E(): 0, (62.6% identity in 436 aa overlap). Contains a possible N-terminal signal or anchor sequence. Predicted to be an outer membrane protein (See Song et al., 2008). Note that previously known as mce2. Q79FY7 No virulence, detoxification, adaptation Q79FY7 Q79FY7 Q79FY7 NC_000962.3 Mycobrowser_v5 CDS 688808 689062 . + 0 Rv0590A Rv0590A Unknown, but could be involved in host cell invasion. Mce-family related protein Rv0590A, len: 84 aa. Probable continuation of mce2B|Rv0590. Can find no frameshift to account for this. Possible nucleotide G missing at 688793 as there are 5 in Mycobacterium bovis but only 4 in CDC1551. Strong similarity to C-terminus of other Mce proteins e.g. AL583926|AL583926_38 from Mycobacterium leprae strain tn (346 aa), FASTA scores: E(): 1.2e-20, (67.85% identity in 84 aa overlap). I6X9D2 No virulence, detoxification, adaptation I6X9D2 NC_000962.3 Mycobrowser_v5 CDS 689059 690504 . + 0 Rv0591 mce2C Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce2C Rv0591, (MTCY19H5.31c), len: 481 aa. Mce2C; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07415|R0171|MTCI28.11|mce1C (515 aa); O53969|Rv1968|MTV051.06|mce3C (410 aa); etc. Also highly similar to others e.g. NP_302658.1|NC_002677 putative secreted protein from Mycobacterium leprae (519 aa); CAC12796.1|AL445327 putative secreted protein from Streptomyces coelicolor (351 aa); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and may contain N-terminal signal or anchor sequence. Has highly Pro-rich C-terminus. O07787 No virulence, detoxification, adaptation O07787 O07787 O07787 NC_000962.3 Mycobrowser_v5 CDS 690501 692027 . + 0 Rv0592 mce2D Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce2D Rv0592, (MTCY19H5.30c), len: 508 aa. Mce2D; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07416|Rv0172|MTCI28.12|mce1D (530 aa); O53970|Rv1969|MTV051.07|mce3D (423 aa); etc. Also highly similar to others e.g. NP_302659.1|NC_002677 putative secreted protein from Mycobacterium leprae (531 aa); CAC12795.1|AL445327 putative secreted protein from Streptomyces coelicolor (337 aa); etc. Has highly Pro-rich C-terminus and may contain N-terminal signal or anchor sequence. Predicted to be an outer membrane protein (See Song et al., 2008). I6WYT7 No virulence, detoxification, adaptation O07786 I6WYT7 I6WYT7 NC_000962.3 Mycobrowser_v5 CDS 692024 693232 . + 0 Rv0593 lprL Unknown, but thought to be involved in host cell invasion. Possible Mce-family lipoprotein LprL (Mce-family lipoprotein Mce2E) Rv0593, (MTCY19H5.29c), len: 402 aa. Possible lprL (alternate gene name: mce2E), lipoprotein which belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07417|LPRK|Rv0173|MTCI28.13|mce1E (390 aa); O53971|LPRM|Rv1970|MTV051.08|mce3E (377 aa); etc. Also highly similar to others e.g. NP_302660.1|NC_002677 putative lipoprotein from Mycobacterium leprae (392 aa); CAC12794.1|AL445327 putative secreted protein from Streptomyces coelicolor (413 aa); etc. Contains possible signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site. I6Y461 No cell wall and cell processes O07785 I6Y461 I6Y461 NC_000962.3 Mycobrowser_v5 CDS 693237 694787 . + 0 Rv0594 mce2F Unknown, but thought involved in host cell invasion. Mce-family protein Mce2F Rv0594, (MTCY19H5.28c), len: 516 aa. Mce2F; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), similar to Mycobacterium tuberculosis proteins O07418|Rv0174|MTCI28.14|mce1F (515 aa); O53972|Rv1971|MTV051.09|mce3F (437 aa); etc. Also highly similar to others e.g. NP_302661.1|NC_002677 putative secreted protein from Mycobacterium leprae (516 aa); AAF74993.1|AF143400_1|AF143400|996A027a protein from Mycobacterium avium (80 aa) (similarity on C-terminus); CAC12793.1|AL445327 putative secreted protein from Streptomyces coelicolor (433 aa); etc. Contains possible N-terminal signal or anchor sequence. Predicted to be an outer membrane protein (See Song et al., 2008). O07784 No virulence, detoxification, adaptation O07784 O07784 O07784 NC_000962.3 Mycobrowser_v5 CDS 694839 695231 . - 0 Rv0595c vapC4 Unknown Possible toxin VapC4 Rv0595c, (MTCY19H5.27), len: 130 aa. Possible vapC4, toxin, part of toxin-antitoxin (TA) operon with Rv0596c, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similar to other conserved hypothetical proteins e.g. Rv0627 (135 aa) and Rv0665 (112 aa) from Mycobacterium tuberculosis; and STBB_PSESM|Q52562 plasmid stability protein from Pseudomonas syringae (139 aa), FASTA scores: opt: 131, E(): 0.0035, (35.2% identity in 88 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07783 No virulence, detoxification, adaptation O07783 O07783 O07783 NC_000962.3 Mycobrowser_v5 CDS 695228 695485 . - 0 Rv0596c vapB4 Unknown Possible antitoxin VapB4 Rv0596c, (MTCY19H5.26), len: 85 aa. Possible vapB4, antitoxin, part of toxin-antitoxin (TA) operon with Rv0595c (See Arcus et al., 2005; Pandey and Gerdes, 2005). Highly similar in part to other M. tuberculosis hypothetical proteins e.g. Rv0626, Rv3181c, Rv3385c, Rv3407, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WF21 No virulence, detoxification, adaptation O07782 P9WF21 P9WF21 NC_000962.3 Mycobrowser_v5 CDS 695668 696903 . - 0 Rv0597c Rv0597c Function unknown Conserved hypothetical protein Rv0597c, (MTCY19H5.25), len: 411 aa. Conserved hypothetical protein, highly similar to Rv3179 conserved hypothetical protein from Mycobacterium tuberculosis (429 aa). Also similar to AAF76191.1|AF271296_1|AF271296 putative ATP/GTP binding protein from Mycobacterium smegmatis (428 aa); Rv2008c|YW09_MYCTU|Q10849 conserved hypothetical protein from Mycobacterium tuberculosis (441 aa), FASTA scores: opt: 270, E(): 3.6e-11, (30.5% identity in 416 aa overlap) (N-terminus longer). Also similar to other hypothetical proteins e.g. NP_085874.1|NC_002679 hypothetical protein from Mesorhizobium loti (435 aa) (N-terminus longer). Contains PS00017 ATP/GTP-binding site motif A (P-loop). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6WYU2 No conserved hypotheticals I6WYU2 I6WYU2 NC_000962.3 Mycobrowser_v5 CDS 697564 697800 . - 0 Rv0599c vapB27 Unknown Possible antitoxin VapB27 Rv0599c, (MTCY19H5.23), len: 78 aa. Possible vapB27, antitoxin, part of toxin-antitoxin (TA) operon with Rv0598c, see Arcus et al. 2005. Similar to others e.g. Rv2595|Y0B6_MYCTU|Q50626 conserved hypothetical protein from Mycobacterium tuberculosis (81 aa), FASTA scores: opt: 160, E(): 6.2e-07, (35.8% identity in 81 aa overlap). N-terminus shows stong similarity with N-terminus of NP_104908.1|NC_002678 hypothetical protein from Mesorhizobium loti (89 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07779 No virulence, detoxification, adaptation O07779 O07779 O07779 NC_000962.3 Mycobrowser_v5 CDS 697904 698410 . - 0 Rv0600c Rv0600c Sensor part of a two component regulatory system. Two component sensor kinase [second part] Rv0600c, (MTCY19H5.22), len: 168 aa (probable partial CDS). Two-component sensor kinase (second part), similar to part (C-termini) of many others e.g. Q04943|AFQ2_STRCO sensor protein afsq2 from Streptomyces coelicolor (535 aa), FASTA scores: opt: 347, E(): 1.9e-12, (33.0% identity in 206 aa overlap); etc. Note that sequence was checked and no errors were detected, which would allow this and the upstream ORF to be joined. Start changed since first submission (- 39 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07778 No regulatory proteins O07778 O07778 GO:0018106,GO:0007165,GO:0000155,GO:0000160,GO:0005524 2.7.13.3 O07778 NC_000962.3 Mycobrowser_v5 CDS 699856 700167 . + 0 Rv0603 Rv0603 Unknown Possible exported protein Rv0603, (MTCY19H5.19c), len: 103 aa. Possible exported protein with hydrophobic stretch at aa 7-29. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07775 No cell wall and cell processes 2KGY O07775 O07775 NC_000962.3 Mycobrowser_v5 CDS 700239 701189 . + 0 Rv0604 lpqO Unknown Probable conserved lipoprotein LpqO Rv0604, (MTCY19H5.18c), len: 316 aa. Probable lpqO, conserved lipoprotein, highly similar to Rv2999|lppY putative lipoprotein from Mycobacterium tuberculosis (321 aa), FASTA scores: opt: 1153, E(): 0, (53.2% identity in 312 aa overlap). Contains probable N-terminal signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6X9E2 No cell wall and cell processes O07774 I6X9E2 NC_000962.3 Mycobrowser_v5 CDS 701406 702014 . + 0 Rv0605 Rv0605 Prevents the cointegration of foreign DNA before integration into the chromosome. Possible resolvase Rv0605, (MTCY19H5.17c), len: 202 aa. Possible resolvase for IS_Y349 element, similar to several Mycobacterial hypothetical proteins and weakly similar to Q52563 resolvase from Pseudomonas syringae (210 aa), FASTA scores: opt: 99, E(): 3.1, (35.7% identity in 98 aa overlap). Contains PS00397 Site-specific recombinases active site and probable helix-turn helix motif from aa 9-30 (Score 1815, +5.37 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6Y890 No insertion seqs and phages O07773 I6Y890 GO:0006310,GO:0000150,GO:0003677 I6Y890 NC_000962.3 Mycobrowser_v5 CDS 702016 702759 . + 0 Rv0606 Rv0606 Thought to be required for the transposition of the insertion element IS_1536. Possible transposase (fragment) Rv0606, (MTCY19H5.16c), len: 247 aa. Possible truncated transposase for IS_1536 element, highly similar to N-terminus of other transposases from Mycobacterium tuberculosis e.g. YX16_MYCTU|Q10809|Rv2885c|MT2953|MTCY274.16c putative transposase from Mycobacterium tuberculosis (460 aa), FASTA scores: opt: 1368, E(): 0, (83.5% identity in 237 aa overlap); Rv2978c, Rv0922, Rv3827c, etc. Also similar to N-terminus of MTV002_57|Rv2792 resolvase from M. tuberculosis (193 aa), FASTA score: (87.4% identity in 238 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07772 No insertion seqs and phages O07772 O07772 NC_000962.3 Mycobrowser_v5 CDS 703486 703887 . + 0 Rv0609 vapC28 Unknown Possible toxin VapC28. Contains PIN domain. Rv0609, (MTCY19H5.13c), len: 133 aa. Possible vapC28, toxin, part of toxin-antitoxin (TA) operon with Rv0608, contains PIN domain, see Arcus et al. 2005. Similar to several Mycobacterium tuberculosis hypothetical proteins e.g. YW37_MYCTU|Q10874|Rv1982c|MT2034|MTCY39.37 conserved hypothetical protein (139 aa), FASTA scores: opt: 262, E(): 8.1e-12, (39.1% identity in 128 aa overlap); MTCY20H10.05|Rv0624|MT0652|MTCY20H10.05 conserved hypothetical protein (131 aa), FASTA score: (42.9% identity in 126 aa overlap), Rv0565c, Rv3854c, etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WF81 No virulence, detoxification, adaptation P67238 P9WF81 P9WF81 NC_000962.3 Mycobrowser_v5 CDS 706324 706929 . + 0 Rv0612 Rv0612 Function unknown Conserved hypothetical protein Rv0612, (MTCY19H5.09c), len: 201 aa. Conserved hypothetical protein, highly similar, but in part, to downstream ORF Rv0609A conserved hypothetical protein from Mycobacterium tuberculosis (75 aa); and showing weak similarity with other hypothetical proteins from Mycobacterium tuberculosis. Note that first start has been taken although this overlaps slightly with the upstream ORF. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6X9E8 No conserved hypotheticals I6X9E8 NC_000962.3 Mycobrowser_v5 CDS 706948 709515 . - 0 Rv0613c Rv0613c Unknown Unknown protein Rv0613c, (MTCY19H5.08), len: 855 aa. Unknown protein. Contains a very short region with strong similarity to several preprotein translocases e.g. P47847|SECA_LISMO preprotein translocase seca subunit (836 aa), FASTA scores: opt: 138, E(): 0.18, (38.6% identity in 70 aa overlap, and 72.7% identity in 22 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6Y897 No conserved hypotheticals O07764 I6Y897 I6Y897 NC_000962.3 Mycobrowser_v5 CDS 709356 710348 . + 0 Rv0614 Rv0614 Function unknown Conserved hypothetical protein Rv0614, (MTCY19H5.07c), len: 330 aa. Conserved hypothetical protein, similar in part to Mycobacterium tuberculosis hypothetical proteins e.g. YY16_MYCTU|Q10685|Rv2077c|MT2137|MTCY49.16c conserved hypothetical protein (323 aa), FASTA scores: opt: 200, E(): 0.00016, (28.3% identity in 269 aa overlap); MTCY9F9_15 FASTA score: (40.3% identity in 144 aa overlap), Rv1949c, Rv2542, etc. Several start sites are possible; first start has been chosen. Note that this ORF overlaps with the upstream ORF. Predicted to be an outer membrane protein (See Song et al., 2008). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O07763 No conserved hypotheticals O07763 NC_000962.3 Mycobrowser_v5 CDS 712716 713807 . + 0 Rv0620 galK Involved in galactose metabolism (leloir pathway) (at the first reaction) [catalytic activity: ATP + D-galactose = ADP + D-galactose 1-phosphate]. Probable galactokinase GalK (galactose kinase) Rv0620, (MTCY19H5.01c, MTCY20H10.01), len: 363 aa. Probable galK, galactokinase, similar to others e.g. P13227|GAL1_STRLI galactokinase from Streptomyces lividans (397 aa); P06976|GAL1_ECOLI galactokinase from Escherichia coli (381 aa), FASTA scores: opt: 669, E(): 0, (35.9% identity in 365 aa overlap); etc. Contains PS00106 Galactokinase signature and PS00560 Serine carboxypeptidases, histidine active site. Belongs to the GHMP kinase family. GALK subfamily. P9WN63 No intermediary metabolism and respiration P96910 P9WN63 GO:0046835,GO:0005737,GO:0004335,GO:0006012,GO:0005524 2.7.1.6 P9WN63 NC_000962.3 Mycobrowser_v5 CDS 791070 791666 . - 0 Rv0691c Rv0691c Involved in transcriptional mechanism Probable transcriptional regulatory protein Rv0691c, (MTCY210.08c), len: 198 aa. Probable transcriptional regulator, highly similar to AAC77476.1|U17129 unknown protein from Rhodococcus erythropolis (185 aa); and showing similarity with putative regulatory proteins eg STMTCREP_1|TCMR_STRGA|P39885 tetracenomycin c transcriptional repressor from Streptomyces glaucescens (226 aa), FASTA scores: opt: 178, E(): 8.5e-06, (27.9% identity in 201 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop) and probable helix-turn helix motifs from aa 34-55 (Score 1100, +2.93 SD) and 151-172 (Score 1124, +3.02 SD). P9WMB7 No regulatory proteins P95037 P9WMB7 GO:0006350,GO:0003700,GO:0006355 P9WMB7 NC_000962.3 Mycobrowser_v5 CDS 720005 721732 . - 0 Rv0629c recD Involved in homologous recombination. Probable exonuclease V (alpha chain) RecD (exodeoxyribonuclease V alpha chain) (exodeoxyribonuclease V polypeptide) Rv0629c, (MTCY20H10.10c), len: 575 aa. Probable recD, exonuclease V, alpha chain (exodeoxyribonuclease V, alpha chain) (see citation below), highly similar to other exonucleases e.g. AF157643_3|AAD46809.1|recD Escherichia coli RecD protein homolog from Mycobacterium smegmatis (554 aa); P04993|EX5A_ECOLI|B2819 exodeoxyribonuclease V 67kd polypeptide (exonuclease V alpha chain) from Escherichia coli strain K12 (608 aa), FASTA scores: opt: 512, E(): 1.9e-24, (36.9% identity in 582 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Consists of three subunits; RECB|Rv0630c, RECC|Rv0631c and RECD. P9WHJ1 No information pathways P96919 P9WHJ1 GO:0006281,GO:0004519,GO:0008854,GO:0009338,GO:0004386,GO:0005524 3.1.11.5 P9WHJ1 NC_000962.3 Mycobrowser_v5 CDS 725013 728306 . - 0 Rv0631c recC Involved in homologous recombination. Probable exonuclease V (gamma chain) RecC (exodeoxyribonuclease V gamma chain)(exodeoxyribonuclease V polypeptide) Rv0631c, (MTCY20H10.12c), len: 1097 aa. Probable recC, exonuclease V, gamma chain (exodeoxyribonuclease V, gamma chain) (see Mizrahi & Andersen 1998), highly similar to other exonucleases e.g. AF157643_1|RecC|AAD46807.1 Escherichia coli RecC protein homolog from Mycobacterium smegmatis (1085 aa); P07648|EX5C_ECOLI|B2822 exodeoxyribonuclease V 125 kDa polypeptide (exonuclease V gamma chain) from Escherichia coli strain K12 (1122 aa), FASTA scores: opt: 954, E(): 0, (29.2% identity in 1109 aa overlap); etc. Consists of three subunits; RECB|Rv0630c, RECC and recd|Rv0629c. The transcription of this CDS seems to be activated specifically in host granulomas (see Ramakrishnan et al., 2000). P9WIQ5 No information pathways P9WIQ5 GO:0006281,GO:0004519,GO:0008854,GO:0009338,GO:0004386,GO:0005524 3.1.11.5 P9WIQ5 NC_000962.3 Mycobrowser_v5 CDS 728583 729278 . - 0 Rv0632c echA3 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Probable enoyl-CoA hydratase EchA3 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv0632c, (MTCY20H10.13c), len: 231 aa. Probable echA3, enoyl-CoA hydratase, almost identical to the MTU88877_1 enoyl-CoA hydratase of Mycobacterium tuberculosis field isolate NTI64719, FASTA score: (92.4% identity in 184 aa overlap). Also similar to others e.g. P24162|ECHH_RHOCA enoyl-CoA hydratase from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (257 aa), FASTA scores: opt: 206, E(): 6.3e-07, (31.5% identity in 232 aa overlap); etc. I6Y8B5 No lipid metabolism P96907 I6Y8B5 GO:0016853,GO:0008152,GO:0004300 4.2.1.17 I6Y8B5 NC_000962.3 Mycobrowser_v5 CDS 730320 731033 . - 0 Rv0634c Rv0634c Thought to be involved in glyoxal pathway. Thiolesterase that catalyses the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid [catalytic activity: (S)-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy acid anion]. Possible glyoxalase II (hydroxyacylglutathione hydrolase) (GLX II) Rv0634c, (MTCY20H10.15c), len: 237 aa. Possible glyoxalase II, equivalent to NP_302290.1|NC_002677 putative glyoxylase II from Mycobacterium leprae (238 aa); and similar to U00011_3|Y0BK_MYCLE|Q49649 hypothetical 23.9 kDa protein from Mycobacterium leprae (218 aa), FASTA scores: opt: 281, E(): 3.9e-12, (31.8% identity in 201 aa overlap). Also similar to other glyoxalases and metallo-beta-lactamase family proteins e.g. NP_386770.1|NC_003047 putative hydroxyacylglutathione hydrolase from Sinorhizobium meliloti (256 aa); etc. Also similar to other putative glyoxylases from Mycobacterium tuberculosis e.g. Rv1637c. Belongs to the glyoxalase II family. Cofactor: binds two zinc ions. I6Y4A5 No virulence, detoxification, adaptation I6Y4A5 GO:0004416 3.1.2.6 I6Y4A5 NC_000962.3 Mycobrowser_v5 CDS 731113 731364 . + 0 Rv0634A Rv0634A Unknown Unknown protein Rv0634A, len: 83 aa. Unknown protein. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WKS5 No conserved hypotheticals Q8VKH3 P9WKS5 P9WKS5 NC_000962.3 Mycobrowser_v5 CDS 732825 733325 . + 0 Rv0637 hadC Involved in fatty acid synthesis type II (fas-II) (3R)-hydroxyacyl-ACP dehydratase subunit HadC Rv0637, (MTCY20H10.18), len: 166 aa. HadC, (3R)-hydroxyacyl-ACP dehydratase subunit, equivalent to NP_302285.1|NC_002677|YV31_MYCLE|P54879 conserved hypothetical protein from Mycobacterium leprae (166 aa), FASTA scores: opt: 352, E(): 4e-19, (39.2% identity in 148 aa overlap); and highly similar to others from Mycobacterium leprae e.g. NP_302287.1|NC_002677 conserved hypothetical protein (159 aa). Also highly similar to CAB77410.1|AL160431|SCD82.07 hypothetical protein from Streptomyces coelicolor (150 aa); Rv0635|NP_215149.1|NC_000962|MTY20H10_17 conserved hypothetical protein (two ORFs upstream) from Mycobacterium tuberculosis (158 aa), FASTA score: (49.3% identity in 150 aa overlap); and Rv0504c|NP_215018.1|NC_000962|YV31_MYCTU|Q11168 hypothetical protein from Mycobacterium tuberculosis (166 aa), FASTA scores: opt: 380, E(): 3.8e-21, (43.1% identity in 137 aa overlap). P9WFJ9 No intermediary metabolism and respiration P9WFJ9 P9WFJ9 NC_000962.3 Mycobrowser_v5 CDS 735517 736224 . + 0 Rv0641 rplA This protein binds directly to 23S ribosomal RNA and is located in the neighborhood of the site where elongation factor TU is bound to the ribosome. 50S ribosomal protein L1 RplA Rv0641, (MTCY20H10.22), len: 235 aa. rplA, 50S ribosomal protein L1, equivalent to NP_302281.1|NC_002677 50S ribosomal protein L1 from Mycobacterium leprae (235 aa). Also highly similar to others e.g. P3625|RL1_STRGR 50s ribosomal protein L1 from Streptomyces griseus (240 aa), FASTA scores: opt: 1081, E(): 0, (72.2% identity in 230 aa overlap); etc. Belongs to the L1P family of ribosomal proteins. P9WHC7 No information pathways P96932 P9WHC7 GO:0015934,GO:0019843,GO:0006417,GO:0003735,GO:0000049,GO:0006412,GO:0006396 P9WHC7 NC_000962.3 Mycobrowser_v5 CDS 1894224 1895342 . - 0 Rv1668c Rv1668c Thought to be involved in active transport of macrolide across the membrane (export). Macrolide antibiotics resistance by an export mechanism. Responsible for energy coupling to the transport system. Probable first part of macrolide-transport ATP-binding protein ABC transporter Rv1668c, (MTV047.04c), len: 372 aa. Probable first part of macrolide-transport ATP-binding protein ABC transporter (see citation below), similar to many ATP-binding proteins ABC transporter e.g. X80735|SEABCT_1|Q54072 Saccharopolyspora erythraea ertX gene (481 aa), FASTA scores: opt: 938, E(): 0, (45.6% identity in 353 aa overlap); etc. Similarity to other NBD components of ABC transporters suggests that Rv1667c and Rv1668c should be contiguous. However, sequence has been checked and no error found, also same sequence in Mycobacterium tuberculosis CSU93 and Mycobacterium bovis. Contains PS00211 ABC transporters family signature and two times PS00017 ATP/GTP-binding site motif A. Belongs to the ATP-binding transport protein family (ABC transporters). O53916 No cell wall and cell processes O53916 O53916 GO:0016887,GO:0005524 O53916 NC_000962.3 Mycobrowser_v5 CDS 736298 737203 . - 0 Rv0642c mmaA4 Involved in mycolic acids modification. Catalyzes unusual S-adenosyl-methionine-dependent transformation of a cis-olefin mycolic acid into a secondary alcohol. Catalyzes introduction of a hydroxyl group at the distal position on mycolic acid chains to produce the hydroxyl mycolate. Mycolic acids represent a major constituent of the mycobacterial cell wall complex. Methyl transfer results in formation of a secondary hydroxy group with an adjacent methyl branch; olefinic mycolic acid methyl transferase. Methoxy mycolic acid synthase 4 MmaA4 (methyl mycolic acid synthase 4) (MMA4) (hydroxy mycolic acid synthase) Rv0642c, (MTCY20H10.23c), len: 301 aa. MmaA4, methoxy mycolic acid synthase 4 (methyltransferase) (see citations below). Equivalent to AAC44876|AAC44876.1|cmaA methyl transferase (mycolic acid modification protein) from Mycobacterium bovis BCG strain Pasteur (298 aa); NP_302280.1|NC_002677 methyl mycolic acid synthase 4 from Mycobacterium leprae (298 aa); and highly similar to others from Mycobacteria e.g. downstream ORF P72027|mmaA3|Rv0643c|MTCY20H10.24c putative methoxy mycolic acid synthase 3 from Mycobacterium tuberculosis (293 aa). Phosphorylated in vitro by PknJ|Rv2088 (See Jang et al., 2010). Q79FX8 No lipid metabolism 2FK7,2FK8,3HA3,3HA5,3HA7 Q79FX8 Q79FX8 GO:0008610,GO:0008825 2.1.1.- Q79FX8 NC_000962.3 Mycobrowser_v5 CDS 739327 740187 . - 0 Rv0645c mmaA1 Involved in mycolic acids modification. Catalyzes unusual S-adenosyl-methionine-dependent transformation of a cis-olefin mycolic acid into a secondary alcohol. Catalyzes introduction of a hydroxyl group at the distal position on mycolic acid chains to produce the hydroxyl mycolate. Mycolic acids represent a major constituent of the mycobacterial cell wall complex. Methyl transfer results in formation of a secondary hydroxy group with an adjacent methyl branch; olefinic mycolic acid methyl transferase. Methoxy mycolic acid synthase 1 MmaA1 (methyl mycolic acid synthase 1) (MMA1) (hydroxy mycolic acid synthase) Rv0645c, (MTCY20H10.26c), len: 286 aa. MmaA1, methoxy mycolic acid synthase 1 (methyltransferase) (see citations below). Equivalent to NP_302279.1|NC_002677 methyl mycolic acid synthase 1 from Mycobacterium leprae (286 aa); and highly similar to others from Mycobacteria e.g. upstream ORF P72028|mmaA4|Rv0642c|MTCY20H10.23c putative methoxy mycolic acid synthase 4 from Mycobacterium tuberculosis (301 aa). P9WPB1 No lipid metabolism P0C5C3 P9WPB1 GO:0008610,GO:0008825 2.1.1.- P9WPB1 NC_000962.3 Mycobrowser_v5 CDS 742719 746366 . + 0 Rv0648 Rv0648 Alpha-mannosidase activity: hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. Alpha-mannosidase Rv0648, (MTCY20H10.29), len: 1215 aa. Alpha-mannosidase (see citation below), showing some similarity to hypothetical proteins and various sugar hydrolases e.g. SYCSLRA_6|Q55528 hypothetical 1 20.4 kDa protein from Synechocystis (1042 aa), FASTA scores: opt: 260, E(): 3.6e-08, (23.4% identity in 602 aa overlap); etc. Contains PS00659 Glycosyl hydrolases family 5 signature. P96937 No intermediary metabolism and respiration P96937 P96937 GO:0030246,GO:0006013,GO:0008270,GO:0004559 3.2.1.- P96937 NC_000962.3 Mycobrowser_v5 CDS 786149 786946 . + 0 Rv0686 Rv0686 Unknown Probable membrane protein Rv0686, (MTCY210.03), len: 265 aa. Probable membrane protein, with hydrophobic N-terminus. I6XVZ6 No cell wall and cell processes I6XVZ6 NC_000962.3 Mycobrowser_v5 CDS 749234 749929 . - 0 Rv0653c Rv0653c Involved in transcriptional mechanism. Possible transcriptional regulatory protein (probably TetR-family) Rv0653c, (MTCI376.23, MTCY20H10.34c), len: 231 aa. Possible transcriptional regulator, TetR family, similar in N-terminus to others e.g. CAC03642.1|AL391338 putative TetR-family transcriptional regulator from Streptomyces coelicolor (190 aa); Q51597 cam repressor from Pseudomonas putida (186 aa), FASTA scores: opt: 150, E(): 0.00085, (27.8% identity in 97 aa overlap); etc. Also some similarity to Mycobacterium tuberculosis hypothetical transcriptional regulators Rv0681 and Rv1816. Contains probable helix-turn helix motif from aa 27-48 (Score 1156, +3.12 SD). P96941 No regulatory proteins P96941 P96941 GO:0006350,GO:0003700,GO:0006355 P96941 NC_000962.3 Mycobrowser_v5 CDS 750000 751505 . + 0 Rv0654 Rv0654 Function unknown; probably involved in cellular metabolism. Probable dioxygenase Rv0654, (MTCI376.22), len: 501 aa. Probable dioxygenase, highly similar to others eg AAK06796.1|AF324838_15|AF324838|SimC5 putative dioxygenase (involved in tetraene formation) from Streptomyces antibioticus (456 aa); CAB56138.1| AL117669 putative dioxygenase from Streptomyces coelicolor (503 aa); T51734 neoxanthin cleavage enzyme (9-cis-epoxy-carotenoid dioxygenase) from Arabidopsis thaliana (538 aa); Q53353 lignostilbene-alpha,beta-dioxygenase from Pseudomonas paucimobilis (Sphingomonas paucimobilis), FASTA scores: opt: 280, E(): 2.3e-11, (28.5% identity in 523 aa overlap); etc. Also some similarity with Rv0913c|MTCY21C12.07c possible dioxygenase from Mycobacterium tuberculosis (501 aa), FASTA score: (29.5% identity in 522 aa overlap). P9WPR5 No intermediary metabolism and respiration O06785 P9WPR5 GO:0016702,GO:0055114 1.-.-.- NC_000962.3 Mycobrowser_v5 CDS 753693 754409 . - 0 Rv0658c Rv0658c Unknown. Supposedly involved in stationary-phase survival. Probable conserved integral membrane protein Rv0658c, (MTCI376.18), len: 238 aa. Probable conserved integral membrane protein, equivalent to a predicted homologous protein from Mycobacterium smegmatis (see citation below), and showing some similarity with P33774|YPRB_ECOLI hypothetical 24.3 kDa protein from Escherichia coli (217 aa), FASTA scores: opt: 174, E(): 5.3e-05, (25.6% identity in 223 aa overlap). Also similar to Rv1863c and Rv0804 from Mycobacterium tuberculosis. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06781 No cell wall and cell processes O06781 O06781 GO:0016020 NC_000962.3 Mycobrowser_v5 CDS 756137 758500 . + 0 Rv0663 atsD Thought to play an important role in the mineralization of sulfates [catalytic activity: a phenol sulfate + H2O = a phenol + sulfate]. Possible arylsulfatase AtsD (aryl-sulfate sulphohydrolase) (arylsulphatase) Rv0663, (MTCI376.13c), len: 787 aa. Possible atsD, arylsulfatase, similar to others e.g. P5169|ARS_PSEAE arylsulfatase from Pseudomonas aeruginosa (532 aa), FASTA scores: opt: 653, E(): 0, (33.1% identity in 544 aa overlap); etc. Also similar to P95059|MTCY210.30|ATSA|Rv0711|MTCY210.30 from Mycobacterium tuberculosis (787 aa), FASTA score: (38.9% identity in 769 aa overlap); and other arylsulfatases from Mycobacterium tuberculosis e.g. Rv3299c|ATSB (970 aa), Rv0711, etc. Contains PS00523 Sulfatases signature 1. Belongs to the sulfatase family. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6XVW9 No intermediary metabolism and respiration O06776 I6XVW9 GO:0008152,GO:0004065 3.1.6.1 I6XVW9 NC_000962.3 Mycobrowser_v5 CDS 787099 787926 . + 0 Rv0687 Rv0687 Function unknown; possibly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv0687, (MTCY210.04), len: 275 aa. Probable short-chain dehydrogenase/reductase, highly similar to various dehydrogenases (generally SDR family) e.g. U17129|RSU17129_7 short-chain dehydrogenase from Rhodococcus erythropolis (275 aa), FASTA scores: opt: 1112, E(): 0, (61.2% identity in 268 aa overlap); MMU34072_2 steroid dehydrogenase from Musmus culus (260 aa), FASTA scores: opt: 390, E(): 2.2e-17, (34.1% identity in 267 aa overlap); etc. Also similar to MTV002_16|O33292|Rv2750 dehydrogenase from Mycobacterium tuberculosis (272 aa). Contains PS00061 Short-chain alcohol dehydrogenase family signature. P9WGS7 No intermediary metabolism and respiration P95033 P9WGS7 GO:0055114,GO:0016491 1.-.-.- P9WGS7 NC_000962.3 Mycobrowser_v5 CDS 763370 767320 . + 0 Rv0668 rpoC Catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [catalytic activity: N nucleoside triphosphate = N diphosphate + {RNA}(N)]. DNA-directed RNA polymerase (beta' chain) RpoC (transcriptase beta' chain) (RNA polymerase beta' subunit). Rv0668, (MTCI376.07c), len: 1316 aa. RpoC, DNA-directed RNA polymerase, beta' chain (see Miller et al., 1994), equivalent to P30761|RPOC_MYCLE|ML1890|S31146 DNA-directed RNA polymerase beta' chain from Mycobacterium leprae (1316 aa), FASTA scores: opt: 8295, E(): 0, (95.6% identity in 1316 aa overlap). Also highly similar to others e.g. CAB77429.1|AL160431 DNA-directed RNA polymerase beta' chain (fragment) from Streptomyces coelicolor (1059 aa); P37871|RPOC_BACSU from Bacillus subtilis (1199 aa), FASTA scores: opt: 2367, E(): 0, (52.9 identity in 1317 aa overlap); etc. Belongs to the RNA polymerase beta' chain family. P9WGY7 No information pathways P0A674 P9WGY7 GO:0003899,GO:0006351,GO:0003677 2.7.7.6 P9WGY7 NC_000962.3 Mycobrowser_v5 CDS 767684 769597 . - 0 Rv0669c Rv0669c Function unknown; hydrolytic enzyme probably involved in cellular metabolism. Possible hydrolase Rv0669c, (MTCI376.05), len: 637 aa. Possible hydrolase, highly similar to various hydrolases (N-terminus shorter) e.g. BAA88409.1|AB028646 alkaline ceramidase from Pseudomonas aeruginosa (670 aa,) FASTA scores: opt: 1490, E(): 0, (41.2% identity in 651 aa overlap); NP_063946.1|NM_019893 mitochondrial ceramidase from Homo sapiens (761 aa); P_446098.1|NM_053646 N-acylsphingosine amidohydrolase 2 from Rattus norvegicus (761 aa); BAB09641.1|AB016885 neutral ceramidase from Arabidopsis thaliana (705 aa); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). O06769 No intermediary metabolism and respiration O06769 GO:0016787 3.-.-.- O06769 NC_000962.3 Mycobrowser_v5 CDS 770582 771424 . + 0 Rv0671 lpqP Unknown Possible conserved lipoprotein LpqP Rv0671, (MTCI376.03c), len: 280 aa. Possible lpqP, conserved lipoprotein, similar to U00012|B1308_F2_43|Q49658 from Mycobacterium leprae (302 aa), FASTA scores: opt: 449, E(): 2.4e-22, (37.6% identity in 242 aa overlap). Also highly similar to lpqC|Rv3298c|MTCY71.38c putative lipoprotein from Mycobacterium tuberculosis (304 aa). Also similar to a large variety of proteins including various esterases and poly(3-hydroxyalkanoate) depolymerases, e.g. NP_249234.1|NC_002516 hypothetical protein from Pseudomonas aeruginosa (322 aa); C-terminus of AAD45376.1|AF164516_1|AF164516 cinnamoyl ester hydrolase EstA from Piromyces equi (536 aa); part of P52090|PHA1_PSELE poly(3-hydroxyalkanoate) depolymerase C precursor from Pseudomonas lemoignei (414 aa); CAC10310.1|AL442629 putative secreted protein from Streptomyces coelicolor (348 aa); etc. Has a 17 aa signal sequence and contains appropriately positioned (PS00013) Prokaryotic membrane lipoprotein lipid attachment site. I6XVY0 No cell wall and cell processes I6XVY0 GO:0016787 NC_000962.3 Mycobrowser_v5 CDS 771484 773112 . + 0 Rv0672 fadE8 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE8 Rv0672, (MTCI376.02c), len: 542 aa. Probable fadE8, acyl-CoA dehydrogenase, highly similar to many e.g. CAC33951.1|AL589708 putative acyl-CoA dehydrogenase from Streptomyces coelicolor (557 aa); P33224|AIDB_ECOLI|B4187 aidb protein (acyl-CoA dehydrogenases family) from Escherichia coli strain K12 (546 aa), FASTA scores: opt: 1369, E(): 0, (44.1% identity in 524 aa overlap); etc. Also similar to several other M. tuberculosis proteins e.g. Rv0154c|MTCI5.28c FASTA score: (26.3% identity in 342 aa overlap); etc. Contains acyl-CoA dehydrogenases signature 2 (PS00073). Belongs to the acyl-CoA dehydrogenases family. I6X9J0 No lipid metabolism O86368 I6X9J0 GO:0055114,GO:0003995 1.3.99.- I6X9J0 NC_000962.3 Mycobrowser_v5 CDS 774783 775574 . + 0 Rv0675 echA5 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Probable enoyl-CoA hydratase EchA5 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv0675, (MTV040.03), len: 263 aa. Probable echA5, enoyl-CoA hydratase, similar to several e.g. NP_252116.1|NC_002516 probable enoyl CoA-hydratase/isomerase from Pseudomonas aeruginosa (256 aa); Q20376 protein similar to enoyl-CoA hydratase from Caenorhabditis elegans (258 aa), FASTA scores: opt: 697, E(): 0, (47.3% identity in 245 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. Z92669|MTCY8D5_17 (262 aa), FASTA scores: opt: 493, E(): 3.6e-25, (39.1% identity in 243 aa overlap); etc. I6Y4E8 No lipid metabolism Q7D9G0 I6Y4E8 GO:0016853,GO:0008152,GO:0004300 4.2.1.17 I6Y4E8 NC_000962.3 Mycobrowser_v5 CDS 781560 781934 . + 0 Rv0682 rpsL Protein S12 is involved in the translation initiation step. 30S ribosomal protein S12 RpsL Rv0682, (MTV040.10), len: 124 aa. rpsL, 30S ribosomal protein S12 (see citations below), equivalent to others from Mycobacteria e.g. P41195|RS12_MYCSM 30S ribosomal protein S12 from Mycobacterium smegmatis (124 aa); P51999|RS12_MYCAV 30S ribosomal protein S12 from Mycobacterium avium (124 aa); etc. Also highly similar to others from other organisms e.g. P97222|RS12_STRCO 30S ribosomal protein S12 from Streptomyces roseosporus, lividans and coelicolor (123 aa); etc. Contains PS00055 Ribosomal protein S12 signature. Belongs to the S12P family of ribosomal proteins. Nucleotide position 781922 in the genome sequence has been corrected, A:G resulting in K121K. P9WH63 No information pathways P41196 P9WH63 GO:0046677,GO:0015935,GO:0003735,GO:0000049,GO:0006412,GO:0019843 SM P9WH63 NC_000962.3 Mycobrowser_v5 CDS 787940 789160 . + 0 Rv0688 Rv0688 Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Putative ferredoxin reductase Rv0688, (MTCY210.05), len: 406 aa. Putative ferredoxin reductase, highly similar to others e.g. BAB55881.1|AB054975 ferredoxin reductase from Terrabacter sp. DBF63 (410 aa); CAC04223.1|AL391515 putative ferredoxin reductase from Streptomyces coelicolor (420 aa); PPU24215_8|Q51973 P-cumate dioxygenase ferredoxin reductase subunit from Pseudomonas putida (402 aa), FASTA scores: opt: 738, E(): 0, (38.8% identity in 330 aa overlap); etc. Also similar to Rv0253 and Rv1869c from Mycobacterium tuberculosis. Could belong to the bacterial type ferredoxin family. P95034 No intermediary metabolism and respiration P95034 P95034 GO:0045454,GO:0005737,GO:0055114,GO:0016491,GO:0050660 1.-.-.- P95034 NC_000962.3 Mycobrowser_v5 CDS 793335 794525 . + 0 Rv0694 lldD1 Involved in respiration; catalyzes conversion of lactate into pyruvate [catalytic activity: (S)-lactate + 2 ferricytochrome C = pyruvate + 2 ferrocytochrome C]. Possible L-lactate dehydrogenase (cytochrome) LldD1 Rv0694, (MTCY210.11), len: 396 aa. Possible lldD1, L-lactate dehydrogenase (cytochrome), similar to NP_302368.1|NC_002677 L-lactate dehydrogenase from Mycobacterium leprae (414 aa). Also similar to others e.g. NP_384560.1|NC_003047 putative L-lactate dehydrogenase (cytochrome) protein from Sinorhizobium meliloti (403 aa); NP_251072.1|NC_002516 L-lactate dehydrogenase from Pseudomonas aeruginosa (383 aa); P33232|LLDD_ECOLI L-lactate dehydrogenase (cytochrome) from Escherichia coli strain K12 (396 aa), FASTA scores: opt: 697, E(): 0, (34.5 identity in 380 aa overlap); etc; and also similar to other oxidoreductases. Note that also highly similar to RSU17129_5|AAC77479.1|U17129 unknown protein from Rhodococcus erythropolis (392 aa), FASTA scores: opt: 2006, E(): 0, (74.1% identity in 386 aa overlap). Also similar to lldD2|Rv1872c|MTCY180.46|MTCY359.01 possible L-lactate dehydrogenase (cytochrome) from Mycobacterium tuberculosis (414 aa). Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenases family. P9WND7 No intermediary metabolism and respiration P95040 P9WND7 GO:0004460,GO:0055114,GO:0010181 1.1.2.3 P9WND7 NC_000962.3 Mycobrowser_v5 CDS 795519 796931 . + 0 Rv0696 Rv0696 Function unknown; probably involved in cellular metabolism. Probable membrane sugar transferase Rv0696, (MTCY210.13), len: 470 aa. Probable membrane sugar transferase, similar (except in N-terminus) to NP_069157.1|NC_000917 glycosyl transferase from Archaeoglobus fulgidus (324 aa); NP_279985.1|NC_002607 rhamnosyl transferase from Halobacterium sp. NRC-1 (299 aa); NP_059113.1|NM_017417 polypeptide N-acetylgalactosaminyltransferase 8 from (637 aa). Note that also highly similar to P46370|YTH1_RHOER hypothetical 55.3 KDA protein from Rhodococcus erythropolis (513 aa), FASTA scores: opt: 1514, E(): 0, (51.8% identity in 469 aa overlap). P9WMX1 No intermediary metabolism and respiration P95042 P9WMX1 GO:0016740 2.-.-.- P9WMX1 NC_000962.3 Mycobrowser_v5 CDS 803411 803692 . + 0 Rv0705 rpsS Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA. 30S ribosomal protein S19 RpsS Rv0705, (MTCY210.24), len: 93 aa. rpsS, 30S ribosomal protein S19, equivalent to S36895 ribosomal protein S19 from Mycobacterium bovis (93 aa), FASTA scores: opt: 623, E(): 0, (98.9% identity in 93 aa overlap); and NP_302261.1|NC_002677 30S ribosomal protein S19 from Mycobacterium leprae (93 aa). Also highly similar to others e.g. CAB82074.1|AL161803 30S ribosomal protein S19 from Streptomyces coelicolor (93 aa); etc. Contains PS00323 Ribosomal protein S19 signature. Belongs to the S19P family of ribosomal proteins. P9WH45 No information pathways P0A5X4 P9WH45 GO:0015935,GO:0003735,GO:0006412,GO:0019843 P9WH45 NC_000962.3 Mycobrowser_v5 CDS 803689 804282 . + 0 Rv0706 rplV This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, E.G., L4, L17, and L20. It is important during the early stages of 50S reconstitution. 50S ribosomal protein L22 RplV Rv0706, (MTCY210.25), len: 197 aa. rplV, 50S ribosomal protein L22, equivalent to O06115|RL22_MYCSM 50S ribosomal protein L22 from Mycobacterium smegmatis (153 aa); MBS10OPER_7 50S ribosomal protein L22 from Mycobacterium bovis BCG; and MLCB2492_7 50S ribosomal protein L22 from Mycobacterium leprae (175 aa). Also highly similar to others e.g. CAB82075.1|AL161803 50S ribosomal protein L22 from Streptomyces coelicolor (125 aa); P42060|RL22_BACSU 50s ribosomal protein L22 from Bacillus subtilis (113 aa), FASTA scores: opt: 368, E(): 2.4e-13, (52.8% identity in 108 aa overlap); etc. Contains PS00464 Ribosomal protein L22 signature, and contains repetitive sequence at C-terminus. Belongs to the L22P family of ribosomal proteins. P9WHC1 No information pathways P61181 P9WHC1 GO:0019843,GO:0003735,GO:0006412,GO:0015934 P9WHC1 NC_000962.3 Mycobrowser_v5 CDS 806335 808698 . + 0 Rv0711 atsA Thought to play an important role in the mineralization of sulfates [catalytic activity: a phenol sulfate + H2O = a phenol + sulfate]. Possible arylsulfatase AtsA (aryl-sulfate sulphohydrolase) (arylsulphatase) Rv0711, (MTCY210.30), len: 787 aa. Possible atsA, arylsulfatase, similar to others e.g. P51691|ARS_PSEAE arylsulfatase from Pseudomonas aeruginosa (532 aa), FASTA scores: opt: 439, E(): 2.9e-21, (30.8% identity in 552 aa overlap); etc. Also similar to other hypothetical arylsulfatases from Mycobacterium tuberculosis e.g. Rv3299c, Rv0663, etc. Contains PS00523 Sulfatases signature 1, and PS00149 Sulfatases signature 2. Belongs to the sulfatase family. P95059 No intermediary metabolism and respiration P95059 P95059 GO:0008152,GO:0004065 3.1.6.1 P95059 NC_000962.3 Mycobrowser_v5 CDS 812976 813374 . + 0 Rv0718 rpsH Binds directly to the central domain of 16S ribosomal RNA. 30S ribosomal protein S8 RpsH Rv0718, (MTCY210.37), len: 132 aa. rpsH, 30S ribosomal protein S8, equivalent to O32997|MLCB2492_18 30S ribosomal protein S8 from Mycobacterium leprae (132 aa). Also highly similar to others e.g. CAB82084.1|AL161803 30S ribosomal protein S8 from Streptomyces coelicolor (132 aa); P33106|RS8_MICLU 30s ribosomal protein S8 from Micrococcus luteus (132 aa), FASTA scores: opt: 669, E(): 0, (77.3% identity in 132 aa overlap); etc. Contains PS00053 Ribosomal protein S8 signature. Belongs to the S8P family of ribosomal proteins. P9WH27 No information pathways P66625 P9WH27 GO:0019843,GO:0005840,GO:0003735,GO:0006412 P9WH27 NC_000962.3 Mycobrowser_v5 CDS 822866 823594 . + 0 Rv0730 Rv0730 Acetylation, substrate unknown GCN5-related N-acetyltransferase Rv0730, (MTV041.04), len: 242 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain in C-terminal part. See Vetting et al. 2005. Equivalent to Z98756|MLCB2492_26 hypothetical protein from Mycobacterium leprae (227 aa), FASTA scores: opt: 1180, E(): 0, (83.5% identity in 218 aa overlap). I6XW38 No intermediary metabolism and respiration I6XW38 GO:0008152,GO:0008080 I6XW38 NC_000962.3 Mycobrowser_v5 CDS 814328 814990 . + 0 Rv0721 rpsE Protein S5 is important in the assembly and function of the 30S ribosomal subunit. 30S ribosomal protein S5 RpsE Rv0721, (MTCY210.40), len: 220 aa. rpsE, 30S ribosomal protein S5, equivalent to MLCB2492_21 ribosomal protein S5 from Mycobacterium leprae (217 aa). Also highly similar to others e.g. P46790|RS5_STRCO 30s ribosomal protein S5 from Streptomyces coelicolor (167 aa), FASTA scores: opt: 889, E(): 0, (82.1% identity in 162 aa overlap); etc. Note N-terminus is extented compared to other rpsE genes. Contains PS00585 Ribosomal protein S5 signature, PTS HPr component phosphorylation sites signature. Belongs to the S5P family of ribosomal proteins. P9WH33 No information pathways P66574 P9WH33 GO:0015935,GO:0003735,GO:0006412,GO:0019843 P9WH33 NC_000962.3 Mycobrowser_v5 CDS 815663 817534 . + 0 Rv0724 sppA Involved in digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane. Possible protease IV SppA (endopeptidase IV) (signal peptide peptidase) Rv0724, (MTCY210.43), len: 623 aa. Possible sppA, protease IV (endopeptidase IV), equivalent (but longer 23 aa) to MLCB2492_24|O33003 endopeptidase IV from Mycobacterium leprae (602 aa). Also similar to others e.g. NP_419743.1|NC_002696 signal peptide peptidase SppA from Caulobacter crescentus (594 aa); P08395|SPPA_ECOLI|B1766 protease IV (endopeptidase) from Escherichia coli strain K-12 (618 aa), FASTA scores: opt: 582, E(): 8.9e-27, (34.1% identity in 525 aa overlap); etc. Belongs to peptidase family S49. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P95072 No cell wall and cell processes P95072 P95072 GO:0008233,GO:0006508,GO:0006465,GO:0016021 3.4.21.- P95072 NC_000962.3 Mycobrowser_v5 CDS 821507 822853 . + 0 Rv0729 xylB Phosphorylates D-xylulose [catalytic activity: ATP + D-xylulose = ADP + D-xylulose 5-phosphate]. Possible D-xylulose kinase XylB (xylulokinase) (xylulose kinase) Rv0729, (MTV041.03), len: 448 aa. Possible xylB, D-xylulose-kinase (xylulokinase). C-terminus highly similar to AAD09880.1|U77912 unknown protein from Mycobacterium bovis (102 aa); and N-terminus highly similar to T45387|Z98756|MLCB2492_25 hypothetical protein from Mycobacterium leprae (110 aa), FASTA scores: opt: 427, E(): 1.1e-19, (60.9% identity in 110 aa overlap). Also similar to xylA/xylB genes from various bacterial species e.g. AAC26499.1|AF045245 D-xylulose-kinase from Klebsiella pneumoniae (487 aa); NP_418021.1|NC_000913 xylulokinase from Escherichia coli strain K12 (484 aa), FASTA scores: opt: 260, E(): 7.5e-09, (25.9% identity in 478 aa overlap); etc. Also similar to Rv3696c|glpK probable glycerol kinase from Mycobacterium tuberculosis (517 aa). Belongs to the fucokinase / gluconokinase / glycerokinase / xylulokinase family. I6Y4K0 No intermediary metabolism and respiration O53793 I6Y4K0 GO:0004856,GO:0005975 2.7.1.17 I6Y4K0 NC_000962.3 Mycobrowser_v5 CDS 824800 826125 . + 0 Rv0732 secY Essential for protein export. Interacts with SECA|Rv3240c and SECE|Rv0638 to allow the translocation of proteins across the plasma membrane, by forming part of a channel. Probable preprotein translocase SecY Rv0732, (MTV041.06), len: 441 aa. Probable SecY, preprotein translocase (integral membrane protein) (see citation below), equivalent to NP_302243.1|NC_002677 SecY subunit of preprotein translocase from Mycobacterium leprae (438 aa); AAC04389.1|AF047021 preprotein translocase subunit from Mycobacterium smegmatis (438 aa); and U77912|MBU77912_1 preprotein translocase subunit from Mycobacterium bovis (441 aa), FASTA scores: opt: 2802, E(): 0, (99.8% identity in 441 aa overlap). Also highly similar to others e.g. P46785|SECY_STRCO preprotein translocase SECY subunit from Streptomyces coelicolor (437 aa); etc. Contains PS00755 and PS00756 protein secY signatures 1 and 2. Belongs to the SECE/SEC61-alpha family. Part of the prokaryotic protein translocation apparatus which comprise SECA|Rv3240c, SECD|Rv2587c, SECE|Rv0638, SECF|Rv2586c, SECG|Rv1440 and SECY. P9WGN3 No cell wall and cell processes P0A5Z2 P9WGN3 GO:0016021,GO:0005886,GO:0015031,GO:0055085,GO:0015450 NC_000962.3 Mycobrowser_v5 CDS 831776 832303 . + 0 Rv0740 Rv0740 Function unknown Conserved hypothetical protein Rv0740, (MTV041.14), len: 175 aa. Conserved hypothetical protein; C-terminus (possibly part of truncated IS1557) shows nearly perfect identity to Rv0750|MTV041_24 (81 aa), FASTA score: (92.6% identity in 81 aa overlap). Also shows weak similarity to MTV007_5 hypothetical protein from Mycobacterium tuberculosis (313 aa), FASTA score: (34.5% identity in 110 aa overlap); and MLCL536_27 hypothetical protein from Mycobacterium leprae (315 aa), FASTA score: (34.5% identity in 84 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53803 No conserved hypotheticals O53803 NC_000962.3 Mycobrowser_v5 CDS 834440 834946 . - 0 Rv0744c Rv0744c Thought to be involved in transcriptional mechanism. Possible transcriptional regulatory protein Rv0744c, (MTV041.18c), len: 168 aa. Possible transcriptional regulator, showing weak similarity with O86661|SC4A2.05 putative two-component sensor from Streptomyces coelicolor (436 aa), FASTA scores: opt: 117, E(): 0.88, (37.25% identity in 94 aa overlap); and some putative excisionases or transposases. Also weakly similar to P71902|YN10_MYCTU|Rv2310|MT2372|MTCY3G12.24c conserved hypothetical protein from Mycobacterium tuberculosis (114 aa); and Q11144|Y477_MYCTU|Rv0477|MT0495|MTCY20G9.03 conserved hypothetical protein from Mycobacterium tuberculosis (148 aa). Equivalent to AAK45006 from Mycobacterium tuberculosis strain CDC1551 (179 aa) but shorter 11 aa. Contains probable helix-turn helix motif from aa 5-26 (Score 1350, +3.78 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53807 No regulatory proteins O53807 GO:0000166,GO:0003677 NC_000962.3 Mycobrowser_v5 CDS 842347 843231 . - 0 Rv0751c mmsB Catalyzes the NAD-dependent, reversible oxidation of 3-hydroxbutyrate to methylmalonate [catalytic activity: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH]. Probable 3-hydroxyisobutyrate dehydrogenase MmsB (hibadh) Rv0751c, (MTV041.25c), len: 294 aa. Probable mmsB, 3-hydroxyisobutyrate dehydrogenase, highly similar to others e.g. NP_102847.1|NC_002678 3-hydroxyisobutyrate dehydrogenase from Mesorhizobium loti (294 aa); NP_420167.1|NC_002696 3-hydroxyisobutyrate dehydrogenase from Caulobacter crescentus (298 aa); A32867 3-hydroxyisobutyrate dehydrogenase from Rattus norvegicus (346 aa); etc. Also similar to methylmalonate semialdehyde dehydrogenases e.g. M84911|PSE MMSRAB_3 methylmalonate semialdehyde dehydrogenase from Pseudomonas aeruginosa (298 aa), FASTA scores: opt: 786, E(): 0, (45.8% identity in 297 aa overlap). Also similar to 6-phosphogluconate dehydrogenases from Mycobacterium tuberculosis e.g. Rv1122 and Rv1844c. Contains PS00895 3-hydroxyisobutyrate dehydrogenase signature. Belongs to the 3-hydroxyisobutyrate dehydrogenase family. P9WNY5 No intermediary metabolism and respiration P63935 P9WNY5 GO:0051287,GO:0055114,GO:0006098,GO:0004616,GO:0006573,GO:0008442 1.1.1.31 P9WNY5 NC_000962.3 Mycobrowser_v5 CDS 844421 845953 . - 0 Rv0753c mmsA Plays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA [catalytic activity: 2-methyl-3-oxopropanoate + CoA + NAD+ = propanoyl-CoA + CO2 + NADH]. Probable methylmalonate-semialdehyde dehydrogenase MmsA (methylmalonic acid semialdehyde dehydrogenase) (MMSDH) Rv0753c, (MTV041.27c), len: 510 aa. Probable mmsA, methylmalonic acid semialdehyde dehydrogenase, highly similar to others e.g. NP_420115.1|NC_002696 putative methylmalonate-semialdehyde dehydrogenase from Caulobacter crescentus (499 aa); L48550|STMMSDA_1|CAB75315.1|AL139164 methylmalonic acid semialdehyde dehydrogenase from Streptomyces coelicolor (500 aa), FASTA score: (51.6% identity in 498 aa overlap); M84911|PSEMMSRAB_2|NP_252260.1|NC_002516 methylmalonate-semialdehyde dehydrogenase from Pseudomonas aeruginosa (497 aa), FASTA scores: opt: 1127, E(): 0, (47.9% identity in 507 aa overlap); etc. Note that also highly similar to malonic semialdehyde oxidative decarboxylases e.g. NP_104968.1|NC_002678 malonic semialdehyde oxidative decarboxylase from Mesorhizobium loti (498 aa); NP_384832.1|NC_003047 putative malonic semialdehyde oxidative decarboxylase protein from Sinorhizobium meliloti (498 aa); etc. Contains PS00070 Aldehyde dehydrogenases cysteine active site. Belongs to the aldehyde dehydrogenases family. O53816 No intermediary metabolism and respiration O53816 O53816 GO:0055114,GO:0006573,GO:0004491 1.2.1.27 O53816 NC_000962.3 Mycobrowser_v5 CDS 851608 852351 . + 0 Rv0757 phoP Involved in transcriptional mechanism. Part of the two component regulatory system PHOP/PHOQ. This protein is thought to be a positive regulator for the phosphate regulon, required for intracellular growth. Transcription of this operon is positively regulated by PHOB and PHOR|Rv0758 when phosphate is limited. Possible two component system response transcriptional positive regulator PhoP Rv0757, (MTCY369.02), len: 247 aa. Possible phoP, two component system response phosphate regulon transcriptional regulator (see citations below), highly similar to various transcriptional regulators e.g. CAC32360.1|AL583945 putative two component system response regulator from Streptomyces coelicolor (271 aa); T45446 probable two-component response regulator from Mycobacterium leprae (253 aa); and similar to phoP proteins e.g. P13792|PHOP_BACSU alkaline phosphatase synthesis transcription regulatory protein from Bacillus subtilis (240 aa), FASTA scores: opt: 594, E(): 2.3e-33, (41.0% identity in 234 aa overlap); etc. Also highly similar to Rv3765c from Mycobacterium tuberculosis (234 aa), Rv1033c (257 aa), RV0903c|MTCY31.31c|Q10531 (236 aa), FASTA score: (45.4% identity in 229 aa overlap); MTCY10G2_16 and MTU88959_1. P71814 No regulatory proteins 2PMU,3R0J P71814 P71814 GO:0006355,GO:0000156,GO:0000160,GO:0003677 P71814 NC_000962.3 Mycobrowser_v5 CDS 852396 853853 . + 0 Rv0758 phoR Sensor part of a two component regulatory system. This protein is thought to be a sensor kinase for the phosphate regulon. Transcription of this operon is positively regulated by PHOB|Rv0757 and PHOR when phosphate is limited. Possible two component system response sensor kinase membrane associated PhoR Rv0758, (MTCY369.03), len: 485 aa. Possible phoR, two component system response phosphate sensor kinase membrane-associated, highly similar to various sensor kinases e.g. CAC32361.1|AL583945 putative two component system histidine kinase from Streptomyces coelicolor (524 aa); NP_349365.1|NC_003030 Membrane-associated sensory histidine kinase with HAMP domain from Clostridium acetobutylicum (482 aa); and similar to phoP proteins e.g. NP_372216.1|NC_002758 alkaline phosphatase synthesis sensor protein from Staphylococcus aureus (554 aa); P23545|PHOR_BACSU alkaline phosphatase synthesis sensor from Bacillus subtilis (579 aa), FASTA scores: opt: 515, E(): 1.9e-25, (40.0% identity in 230 aa overlap); etc. Also similar to proteins from Mycobacterium tuberculosis e.g. MTCY20G9.16 FASTA scores: (34.5% identity in 264 aa overlap), MTU88959_2 (509 aa), MTCY10G2_17, etc. P71815 No regulatory proteins P71815 P71815 GO:0016021,GO:0018106,GO:0007165,GO:0000155,GO:0000160,GO:0005524 2.7.13.3 P71815 NC_000962.3 Mycobrowser_v5 CDS 902111 903694 . + 0 Rv0808 purF Involved in de novo purine biosynthesis (at the first step) [catalytic activity: 5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O]. Amidophosphoribosyltransferase PurF (glutamine phosphoribosylpyrophosphate amidotransferase) (ATASE) (gpatase) Rv0808, (MTCY07H7A.01c), len: 527 aa. PurF, amidophosphoribosyltransferase, equivalent to MLCB5_32|Q50028|PURF from Mycobacterium leprae (556 aa), FASTA scores: (91.3% identity in 518 aa overlap); and CAB96578.1|AJ278609 phosphoribosyl pyrophosphate amidotransferase from Mycobacterium smegmatis (511 aa)(see citation below). Also highly similar to others e.g. BAA89439.1|AB003158 amidophosphoribosyl transferase from Corynebacterium ammoniagenes (490 aa); P00497|PUR1_BACSU amidophosphoribosyltransferase precursor from Bacillus subtilis (476 aa), FASTA scores: opt: 1412, E(): 0, (46.2% identity in 470 aa overlap); etc. Contains PS00103 Purine/pyrimidine phosphoribosyl transferases signature. Belongs to the purine/pyrimidine phosphoribosyltransferase family. P9WHQ7 No intermediary metabolism and respiration P65829 P9WHQ7 GO:0004044,GO:0006541,GO:0046872,GO:0009116,GO:0009113,GO:0006164,GO:0051539 2.4.2.14 P9WHQ7 NC_000962.3 Mycobrowser_v5 CDS 854699 855826 . - 0 Rv0761c adhB Thought to catalyze the reversible oxidation of ethanol to acetaldehyde with the concomitant reduction of NAD. Probably acts on primary or secondary alcohols or hemiacetals [catalytic activity: an alcohol + NAD+ = an aldehyde or ketone + NADH]. Possible zinc-containing alcohol dehydrogenase NAD dependent AdhB Rv0761c, (MTCY369.06c), len: 375 aa. Possible adhB, zinc-containing alcohol dehydrogenase NAD-dependent, similar to others e.g. AAC15839.1|AF060871_4 hypothetical alcohol dehydrogenase from Rhodococcus rhodochrous (370 aa), FASTA scores: opt: 1234, E(): 0, (46.8% identity in 370 aa overlap); P80468|ADH2_STRCA alcohol dehydrogenase II from Struthio camelus (Ostrich) (379 aa); Q03505|ADH1_RABIT alcohol dehydrogenase alpha chain from Oryctolagus cuniculus (Rabbit) (374 aa), FASTA scores: opt: 872, E(): 0, (39.1% identity in 379 aa overlap); etc. Also similar to adhD alcohol dehydrogenase from Mycobacterium tuberculosis (368 aa). Contains PS00059 Zinc-containing alcohol dehydrogenases signature. Belongs to the zinc-containing alcohol dehydrogenase family. P9WQC7 No intermediary metabolism and respiration P71818 P9WQC7 GO:0005737,GO:0055114,GO:0008270,GO:0004022 1.1.1.1 P9WQC7 NC_000962.3 Mycobrowser_v5 CDS 856682 858037 . - 0 Rv0764c cyp51 Involved in sterol biosynthesis. Its precise biological substrate is not known. Catalyzes C14-demethylation of lanosterol, 24,25-dihydrolanosterol and obtusifoliol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol. Cytochrome P450 51 Cyp51 (CYPL1) (P450-L1A1) (sterol 14-alpha demethylase) (lanosterol 14-alpha demethylase) (P450-14DM) Rv0764c, (MT0788, MTCY369.09c), len: 451 aa. Cyp51, cytochrome P450 51 (sterol 14-alpha demethylase), similar to others e.g. Q16850|CP51_HUMAN cytochrome P450 51 (CYPL1) (P450L1) (sterol 14-alpha demethylase) (lanosterol 14-alpha demethylase) from Homo sapiens (509 aa), FASTA scores: opt: 848, E(): 0, (33.9% identity in 439 aa overlap); NP_172633.1|NC_003070 putative obtusifoliol 14-alpha demethylase from Arabidopsis thaliana (488 aa); P93596|CP51_WHEAT cytochrome P450 51 (CYPL1) (P450-L1A1) (obtusifoliol 14-alpha demethylase) from Triticum aestivum (453 aa); etc. Also similar to many other Mycobacterium tuberculosis cytochromes P450 e.g. Rv1394c, FASTA score: (22.5% identity in 444 aa overlap). Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. Belongs to the cytochrome P450 family. P9WPP9 No intermediary metabolism and respiration 1E9X,1EA1,1H5Z,1U13,1X8V,2BZ9,2CI0,2CIB,2VKU,2W09,2W0A,2W0B P0A512 P9WPP9 GO:0009055,GO:0020037,GO:0055114,GO:0008398,GO:0016126,GO:0005737 1.14.13.70 P9WPP9 NC_000962.3 Mycobrowser_v5 CDS 860912 862381 . + 0 Rv0768 aldA Oxidizes a variety of aldehydes [catalytic activity: an aldehyde + NAD+ + H2O = an acid + NADH]. Probable aldehyde dehydrogenase NAD dependent AldA (aldehyde dehydrogenase [NAD+]) Rv0768, (MTCY369.13), len: 489 aa. Probable aldA, NAD-dependent aldehyde dehydrogenase, highly similar to others e.g. AAL14238.1|AY052630 6-oxolauric acid dehydrogenase from Rhodococcus ruber (474 aa); NP_285450.1|NC_001264 aldehyde dehydrogenase from Deinococcus radiodurans (495 aa); NP_241405.1|NC_002570 NADP-dependent aldehyde dehydrogenase from Bacillus halodurans (498 aa); P42757|DHAB_ATRHO betaine-aldehyde dehydrogenase precursor from Atriplex hortensis (Mountain spinach) (502 aa), FASTA scores: opt: 1001, E(): 0, (35.6% identity in 486 aa overlap); etc. Also highly similar to Rv0223c aldehyde dehydrogenase from Mycobacterium tuberculosis (487 aa). Contains PS00687 Aldehyde dehydrogenases glutamic acid active site. Belongs to the aldehyde dehydrogenases family. I6X9R9 No intermediary metabolism and respiration P71823 I6X9R9 GO:0016491,GO:0055114 1.2.1.- I6X9R9 NC_000962.3 Mycobrowser_v5 CDS 862412 863158 . + 0 Rv0769 Rv0769 Function unknown; probably involved in cellular metabolism. Probable dehydrogenase/reductase Rv0769, (MTCY369.14), len: 248 aa. Probable dehydrogenase/reductase, similar to others, especially short-chain type dehydrogenases/reductases and 3-oxoacyl-(acyl-carrier protein) reductases e.g. NP_106890.1|NC_002678 probable short-chain type dehydrogenase/reductase from Mesorhizobium loti (374 aa); NP_243357.1|NC_002570 3-oxoacyl-(acyl-carrier protein) reductase from Bacillus halodurans (246 aa); P28643|FABG_CUPLA 3-oxoacyl-[acyl-carrier protein] reductase from Cuphea lanceolata (320 aa); P25529|HDHA_ECOLI 7-alpha-hydroxysteroid dehydrogenase from Escherichia coli (255 aa), FASTA scores: opt: 536, E(): 6.5e-27, (37.7% identity in 247 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. MTCY02B10.14, FASTA score: (33.7% identity in 249 aa overlap); etc. P9WGQ9 No intermediary metabolism and respiration P71824 P9WGQ9 GO:0055114,GO:0016491 1.-.-.- P9WGQ9 NC_000962.3 Mycobrowser_v5 CDS 864586 865854 . + 0 Rv0772 purD Involved in de novo purine biosynthesis (at the second step) [catalytic activity: ATP + 5-phosphoribosylamine + glycine = ADP + phosphate + 5'-phosphoribosylglycinamide]. Probable phosphoribosylamine--glycine ligase PurD (GARS) (glycinamide ribonucleotide synthetase) (phosphoribosylglycinamide synthetase) (5'-phosphoribosylglycinamide synthetase) Rv0772, (MTCY369.17), len: 422 aa. Probable purD, phosphoribosylamine--glycine ligase, equivalent to Q50144|PURD|PUR2_MYCLE|ML2235|MLCB5.08 phosphoribosylamine--glycine ligase from Mycobacterium leprae (422 aa), FASTA scores: opt: 2272, E(): 0, (81.8% identity in 422 aa overlap). Also highly similar to others e.g. CAB56348.1|AL118514 phosphoribosylamine-glycine ligase from Streptomyces coelicolor (416 aa); P1564|PUR2_ECOLI phosphoribosylamine--glycine ligase from Escherichia coli (429 aa), FASTA scores: opt: 1039, E(): 0, (42.7% identity in 431 aa overlap); etc. Belongs to the GarS family. P9WHM9 No intermediary metabolism and respiration P65893 P9WHM9 GO:0046872,GO:0004637,GO:0009113,GO:0006164,GO:0005524 6.3.4.13 P9WHM9 NC_000962.3 Mycobrowser_v5 CDS 996524 997705 . + 0 Rv0894 Rv0894 Thought to be involved in transcriptional mechanism. Possible transcriptional regulatory protein (possibly LuxR-family) Rv0894, (MTCY31.22), len: 393 aa. Possible regulatory protein, LuxR family, highly similar in part to NP_302202.1|NC_002677 possible transcriptional regulator from Mycobacterium leprae (1106 aa). Also similar to others e.g. CAB95788.1|AL359949 putative multi-domain regulatory protein from Streptomyces coelicolor (780 aa); NP_107293.1|NC_002678 transcriptional regulator from Mesorhizobium loti (903 aa); etc. Also similar to other regulatory proteins from Mycobacterium tuberculosis e.g. Rv2488c|MTV008_44 (1137 aa), FASTA score: (53.2% identity in 363 aa overlap); Rv1358|MTCY02B10_22 (1159 aa), FASTA score: (52.3% identity in 365 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WKP9 No regulatory proteins P9WKP9 GO:0005524 P9WKP9 NC_000962.3 Mycobrowser_v5 CDS 870008 871426 . + 0 Rv0777 purB Involved in de novo purine biosynthesis (at the eight step) [catalytic activity: 1-(5-phosphoribosyl)-4-(N-succino-carboxamide) -5-aminoimidazole = fumarate + 5'-phosphoribosyl-5-amino-4-imidazolecarboxamide (also catalyzes: N6-(1,2-dicarboxyethyl)AMP = fumarate + AMP)]. Probable adenylosuccinate lyase PurB (adenylosuccinase) (ASL) (ASASE) Rv0777, (MTCY369.21b), len: 472 aa. Probable purB, adenylosuccinate lyase, equivalent (but shorter 15 aa) to MLCB5.13|Z95151|g2076607|PURB adenylosuccinate lyase from Mycobacterium leprae (487 aa), FASTA scores: opt: 2640, E(): 0, (86.7% identity in 472 aa overlap). More similar to eukaryotic adenylosuccinate lyases than to prokaryotic adenylosuccinate lyases e.g. P54822|PUR8_MOUSE adenylosuccinate lyase from Mus musculus (484 aa), FASTA scores: opt: 762, E(): 0, (32.4% identity in 445 aa overlap); CAB99134.1|AL390188 putative adenylosuccino lyase (fragment) from Streptomyces coelicolor (362 aa); etc. Contains PS00163 Fumarate lyases signature. Belongs to the lyase 1 family, adenylossucinate lyase subfamily. I6XWA1 No intermediary metabolism and respiration P71832 I6XWA1 GO:0004018,GO:0009152,GO:0070626 4.3.2.2 I6XWA1 NC_000962.3 Mycobrowser_v5 CDS 873343 874236 . + 0 Rv0780 purC Involved in de novo purine biosynthesis (at the seventh step) [catalytic activity: ATP + 1-(5-phosphoribosyl)-4-carboxy-5-aminoimidazole + L-aspartate = ADP + phosphate + 1-(5-phosphoribosyl)-4-(N-succino-carboxamide)-5-aminoimidazole]. Phosphoribosylaminoimidazole- succinocarboxamide synthase PurC (SAICAR synthetase) Rv0780, (MTCY369.24), len: 297 aa. PurC, phosphoribosylaminoimidazole- succinocarboxamide synthase (see citations below), equivalent to MTU34957_1|PURC phosphoribosylaminoimidazole-succinocarboxamide synthase from Mycobacterium leprae (297 aa), FASTA scores: opt: 1986, E(): 0, (99.3% identity in 297 aa overlap). Also similar to others e.g. CAB56351.1|AL118514 phosphoribosylaminoimidazole-succinocarboxamide synthase from Streptomyces coelicolor (299 aa); etc. Contains PS01058 SAICAR synthetase signature 2. Belongs to the SAICAR synthetase family. P9WHN1 No intermediary metabolism and respiration P0A5T4 P9WHN1 GO:0004639,GO:0006164,GO:0005524 6.3.2.6 P9WHN1 NC_000962.3 Mycobrowser_v5 CDS 874233 874943 . + 0 Rv0781 ptrBa Cleaves peptide bonds on the C-terminal side of lysyl and argininyl residues [catalytic activity: hydrolysis of ARG-|-XAA and LYS-|-XAA bonds in oligopeptides, even when P1' residue is proline]. Probable protease II PtrBa [first part] (oligopeptidase B) Rv0781, (MTCY369.25), len: 236 aa. Probable ptrBa, first part of protease II, equivalent to N-terminus of NP_302455.1|NC_002677 protease II from Mycobacterium leprae (724 aa). Also highly similar to N-termini of many proteases II e.g. P24555|PTRB_ECOLI|TLP|B1845 protease II from Escherichia coli strains K12 and HB101 (707 aa), FASTA scores: opt: 204, E(): 7.4e-07, (29.6% identity in 230 aa overlap); etc. ORFs Rv0782 and Rv0781 appear to be a frameshifted homologues of protease II, but we can find no error in the cosmid sequence to account for this. Belongs to peptidase family S9A; also known as the prolyl oligopeptidase family. Note that previously known as ptrBb. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P71835 No intermediary metabolism and respiration P71835 P71835 GO:0004252,GO:0006508 3.4.21.83 P71835 NC_000962.3 Mycobrowser_v5 CDS 876818 878440 . - 0 Rv0783c emrB Translocase that confers resistance to substances of high hydrophobicity. Involved in transport of multidrug across the membrane (export): multidrug resistance by an export mechanism. Responsible for the translocation of the substrate across the membrane. Possible multidrug resistance integral membrane efflux protein EmrB Rv0783c, (MTCY369.27c), len: 540 aa. Possible emrB, integral membrane drug efflux protein, member of major facilitator superfamily (MFS), equivalent to AAL16083.1|AF421382_1|AF421382 EmrB efflux protein from Mycobacterium avium (538 aa). Also similar to other membrane proteins e.g. CAB61606.1|AL133210 putative export protein from Streptomyces coelicolor (496 aa); NP_108371.1|NC_002678 efflux pump protein FarB from Mesorhizobium loti (511 aa); P44927|EMRB_HAEINHI0897| multidrug resistance protein b homologue from Haemophilus influenzae (510 aa), FASTA scores: opt: 706, E(): 1.3e-36, (30.4% identity in 408 aa overlap); etc. Also similar to Rv2333c|MTCY3G12.01 from Mycobacterium tuberculosis (537 aa), FASTA score: (28.2% identity in 408 aa overlap); and Rv1410c|MTCY21B4.27c from Mycobacterium tuberculosis (518 aa), FASTA score: (26.8% identity in 496 aa overlap). Belongs to the major facilitator family; also known as the drug resistance translocase family. P9WG89 No cell wall and cell processes P71836 P9WG89 GO:0005886,GO:0055085,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 879340 881040 . + 0 Rv0785 Rv0785 Function unknown Conserved protein Rv0785, (MTCY369.29), len: 566 aa. Conserved protein, highly similar to other conserved hypothetical proteins e.g. NP_105777.1| NC_002678 hypothetical protein from Mesorhizobium loti (552 aa); SC5F8.14|CAB93742.1|AL357613 conserved hypothetical protein from Streptomyces coelicolor (557 aa); AE001863|AE001863_31 from Deinococcus radiodurans (554 aa), FASTA scores: opt: 2243, E(): 0, (61.1% identity in 550 aa overlap); YEF7_YEAST|P32614 hypothetical 50.8 kd protein (470 aa), FASTA scores: opt: 169, E(): 0.0014, (23.8% identity in 542 aa overlap); etc. Also similar to Rv1817|MTCY1A11.26c from Mycobacterium tuberculosis (487 aa), FASTA score: (26.7% identity in 587 aa overlap). And shows similarity with other dehydrogenases. P71838 No conserved hypotheticals P71838 P71838 GO:0016491,GO:0009055 P71838 NC_000962.3 Mycobrowser_v5 CDS 887137 888636 . - 0 Rv0794c Rv0794c Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv0794c, (MTV042.04c), len: 499 aa. Probable oxidoreductase, possibly dihydrolipoamide dehydrogenase or mercuric reductase. Highly similar to CAB62675.1|AL133422 probable oxidoreductase from Streptomyces coelicolor (477 aa); and similar to various oxidoreductases e.g. P08663|MERA_STAAU mercuric reductase (HG(II) reductase) from Staphylococcus aureus (547 aa); AAK70920.1|AC087551_19|AC087551 putative lipoamide dehydrogenase from Oryza sativa (563 aa); NP_437349.1|NC_003078 putative FAD-dependent pyridine nucleotide-disulphide oxidoreductase, similar to mercuric reductases protein from Sinorhizobium meliloti (473 aa); Q04829|DLDH_HALVO dihydrolipoamide dehydrogenase from Haloferax volcanii (475 aa); P08332|MERA_SHIFL mercuric reductase (564 aa), FASTA scores: opt: 522, E(): 3.7e-26, (31.7% identity in 467 aa overlap); P72740|DLDH_SYNY3|Q53395|LPDA|PDHD|SLR1096 dihydrolipoamide dehydrogenase from Synechocystis sp. strain PCC 6803 (474 aa), FASTA scores: opt: 602, E(): 2.3e-31, (31.0% identity in 493 aa overlap); etc. Note that previously known as lpdB. I6Y4U4 No intermediary metabolism and respiration Q79FW1 I6Y4U4 GO:0045454,GO:0005737,GO:0055114,GO:0016491,GO:0050660 1.-.-.- I6Y4U4 NC_000962.3 Mycobrowser_v5 CDS 889347 890333 . + 0 Rv0796 Rv0796 Required for the transposition of the insertion element IS6110. Putative transposase for insertion sequence element IS6110 Rv0796, (MTV042.06), len: 328 aa. Putative transposase for IS6110. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv0795 and Rv0796, the sequence UUUUAAAG (directly upstream of Rv0796) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 50 aa). P9WKH9 No insertion seqs and phages P0C5G8 P9WKH9 GO:0015074,GO:0006310,GO:0032196,GO:0003677 P9WKH9 NC_000962.3 Mycobrowser_v5 CDS 892266 893273 . - 0 Rv0799c Rv0799c Function unknown Conserved protein Rv0799c, (MTCY07H7A.10, MTCI429B.01), len: 335 aa. Conserved protein, similar to Q50021|U2266C from Mycobacterium leprae (146 aa), FASTA scores: opt: 147, E(): 0.0016, (33.3% identity in 117 aa overlap); Q50020|U2266B from Mycobacterium leprae (27 aa), FASTA scores: opt: 94, E(): 1.3, (56.5% identity in 23 aa overlap). Also highly similar to others e.g. CAC01593.1|AL391041 conserved hypothetical protein from Streptomyces coelicolor (316 aa); AF088897|AF088897_9 hypothetical protein from Zymomonas mobilis (322 aa), FASTA scores: opt: 1132, E(): 0, (56.1% identity in 303 aa overlap); P76536|ECAE000330_8 hypothetical protein from Escherichia coli strain K-12 (308 aa), FASTA scores: E(): 2.2e-30, (37.4% identity in 297 aa overlap); etc. Also similar to some tyrA proteins. Predicted to be an outer membrane protein (See Song et al., 2008). I6Y4U9 No conserved hypotheticals O07180 I6Y4U9 I6Y4U9 NC_000962.3 Mycobrowser_v5 CDS 895820 898084 . + 0 Rv0803 purL Involved in de novo purine biosynthesis (at the fourth step) [catalytic activity: ATP + 5'-phosphoribosylformylglycinamide + L-glutamine + H(2)O = ADP + phosphate + 5'-phosphoribosylformylglycinamidine + L-glutamate]. Phosphoribosylformylglycinamidine synthase II PurL (FGAM synthase II) Rv0803, (MTCY07H7A.06c), len: 754 aa. PurL, phosphoribosylformylglycinamidine synthase II (see citations below), equivalent to NP_302451.1|NC_002677 phosphoribosylformylglycinamidine synthase II from Mycobacterium leprae (754 aa). Also highly similar to others e.g. Q9RKK5|PURL_STRCO from Streptomyces coelicolor (752 aa); P12042|PURL_BACSU from Bacillus subtilis (742 aa), FASTA score: (44.7% identity in 716 aa); etc. Start was chosen by similarity. Belongs to the FGAMS family. P9WHL7 No intermediary metabolism and respiration P0A5T8 P9WHL7 GO:0005524,GO:0005737,GO:0004642,GO:0006189 6.3.5.3 P9WHL7 NC_000962.3 Mycobrowser_v5 CDS 899732 901330 . - 0 Rv0806c cpsY Thought to be involved in exopolysaccharide and/or lipopolysaccharide biosynthetic pathway [catalytic activity: UDP-glucose = UDP-galactose]. Possible UDP-glucose-4-epimerase CpsY (galactowaldenase) (UDP-galactose-4-epimerase) (uridine diphosphate galactose-4-epimerase) (uridine diphospho-galactose-4-epimerase) Rv0806c, (MTCY07H7A.03), len: 532 aa. Possible cpsY, UDP-glucose-4-epimerase, equivalent to Q50025|CPSY probable UDP-glucose-4-epimerase from Mycobacterium leprae (542 aa), FASTA scores: opt: 2964, E(): 0, (82.3% identity in 530 aa overlap). Also similar to AAC38286.1|AF019760|SACB CpsY homolog (involved in meningococcal capsule biosynthesis) from Neisseria meningitidis serogroup a (545 aa); Q51151 capsule gene complex UPD-glucose-4-epimerase (gale) from Neisseria meningitidis (373 aa), FASTA scores: opt: 496, E(): 9.5e-27, (29.3% identity in 358 aa overlap); C-terminus of CAB75373.1|AL139298 putative transferase from Streptomyces coelicolor (942 aa); and many hypothetical proteins from Streptomyces coelicolor. Seems to belong to the sugar epimerase family. P9WGD1 No cell wall and cell processes P9WGD1 GO:0016740,GO:0000271 2.7.-.- NC_000962.3 Mycobrowser_v5 CDS 1024684 1025184 . + 0 Rv0919 Rv0919 Acetylation, substrate unknown GCN5-related N-acetyltransferase Rv0919, (MTCY21C12.13), len: 166 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Some similarity to Q50115 hypothetical protein from Mycobacterium leprae (90 aa), FASTA scores: opt: 243, E(): 5.3e-11, (56.5% identity in 85 aa overlap). Alternative nucleotide at position 1025106 (T->C; F141F) has been observed. I6XA42 No intermediary metabolism and respiration O05911 I6XA42 GO:0008152,GO:0008080 I6XA42 NC_000962.3 Mycobrowser_v5 CDS 908485 909318 . - 0 Rv0815c cysA2 May be a sulfotransferase involved in the formation of thiosulfate [catalytic activity: thiosulfate + cyanide = sulfite + thiocyanate]. Probable thiosulfate sulfurtransferase CysA2 (rhodanese-like protein) (thiosulfate cyanide transsulfurase) (thiosulfate thiotransferase) Rv0815c, (MTV043.07c, MT0837, O05793), len: 277 aa. Probable cysA2 (alternate gene name: sseC4), thiosulfate sulfurtransferase (see Wooff et al., 2002), equivalent to Q50036|CYSA|CYSA3|ML2198|THTR_MYCLE putative sulfurtransferase thiosulfate from Mycobacterium leprae (277 aa). Also highly similar to other putative thiosulfate sulfurtransferases e.g. P16385|THTR_SACER putative thiosulfate sulfurtransferase from Saccharopolyspora erythraea (Streptomyces erythraeus) (281 aa); NP_293941.1|NC_001263 thiosulfate sulfurtransferase from Deinococcus radiodurans (286 aa); etc. Identical second copy present as Rv3117|MTCY164.27|MT3199|O05793|cysA3 (277 aa) (100.0% identity in 277 aa overlap). Contains PS00683 Rhodanese C-terminal signature at C-terminus. Belongs to the rhodanese family. P9WHF9 No intermediary metabolism and respiration 3AAX,3AAY,3HWI O05793 P9WHF9 GO:0004792,GO:0008272 2.8.1.1 P9WHF9 NC_000962.3 Mycobrowser_v5 CDS 909611 910033 . - 0 Rv0816c thiX Thioredoxin participates in various redox reactions through the reversible oxidation of its active center dithiol, to a disulfide, & catalyzes dithiol-disulfide exchange reactions. Probable thioredoxin ThiX Rv0816c, (MTV043.08c), len: 140 aa. Probable thiX, thioredoxin, equivalent to ThiX|U15182|MLU15182_21 thioredoxin from Mycobacterium leprae (172 aa), FASTA scores: opt: 556, E(): 8.8e-31, (63.8% identity in 141 aa overlap); and similar to AAL08576.1|AF418548_2|AF418548 thioredoxin from Mycobacterium avium subsp. paratuberculosis (117 aa). Also similar to other bacterial thioredoxins e.g. CAB95303.1|AL359779 putative thioredoxin from Streptomyces coelicolor (126 aa); P33791|THIO_STRAU|TRX|TRXA thioredoxin from Streptomyces aureofaciens (106 aa); etc. And similar to Rv3914|MT4033|MTV028.05|NP_218431.1|NC_000962|trxC thioredoxin (TRX) (MPT46) from Mycobacterium tuberculosis (116 aa). Has hydrophobic stretch at N-terminus. Seems to belong to the thioredoxin family. I6Y8V2 No intermediary metabolism and respiration O53828 I6Y8V2 GO:0055114,GO:0016491,GO:0045454 1.-.-.- I6Y8V2 NC_000962.3 Mycobrowser_v5 CDS 913558 914199 . - 0 Rv0821c phoY2 Involved in transcriptional regulation of active transport of inorganic phosphate across the membrane. Probable phosphate-transport system transcriptional regulatory protein PhoY2 Rv0821c, (MTV043.13c), len: 213 aa. Probable phoY2, phosphate-transport system regulatory protein, highly similar to PhoY|MLU15182_29|U15182 phosphate transport system regulator from Mycobacterium leprae (222 aa), FASTA scores: opt: 1268, E(): 0, (93.0% identity in 213 aa overlap). Also similar to others e.g. NP_384620.1|NC_003047 probable phosphate transport system transcriptional regulator protein from Sinorhizobium meliloti (237 aa); etc. Also highly similar to MTCI418A.03c|Z96070|PhoY1 probable phosphate transport system transcriptional regulator protein from Mycobacterium tuberculosis (221 aa), FASTA scores: opt: 937, E(): 0, (63.4% identity in 213 aa overlap). Belongs to the PhoU family. P9WI95 No cell wall and cell processes P65720 P9WI95 GO:0006817,GO:0005737 P9WI95 NC_000962.3 Mycobrowser_v5 CDS 914257 916311 . - 0 Rv0822c Rv0822c Function unknown Conserved protein Rv0822c, (MTV043.14c), len: 684 aa. Conserved protein, highly similar in the region between aa 370 - 580 to U2266O|U15182|MLU15182_30 hypothetical protein from Mycobacterium leprae (222 aa), FASTA scores: opt: 819, E(): 0, (60.6% identity in 221 aa overlap). More extended similarity to Rv3267|Z92771|MTCY71_7 from Mycobacterium tuberculosis (498 aa), FASTA scores: opt: 434, E(): 2.2e-17, (26.6% identity in 541 aa overlap), and Rv3484. Also similar to various proteins, preferiously putative membrane proteins and membrane-bound regulatory proteins e.g. CAC44512.1|AL596138 putative membrane protein from Streptomyces coelicolor (524 aa); U56901|BSU56901_1 regulatory protein from Bacillus subtilis (391 aa), FASTA scores: opt: 225, E(): 1.3e-05, (24.7% identity in 340 aa overlap). Contains hydrophobic stretch (aa ~ 160-195) and PS00041 Bacterial regulatory proteins, araC family signature. I6WZI4 No conserved hypotheticals O53834 I6WZI4 I6WZI4 NC_000962.3 Mycobrowser_v5 CDS 925361 927610 . + 0 Rv0833 PE_PGRS13 Function unknown PE-PGRS family protein PE_PGRS13 Rv0833, (MTV043.25), len: 749 aa. PE_PGRS13, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), but lacking N-terminal domain (present in preceding ORF), possibly due to frameshift. Similar in part to many others e.g. MTCY28_25|Z95890 (914 aa), FASTA scores: opt: 2726, E(): 0, (60.1% identity in 776 aa overlap); etc. Q79FV7 No PE/PPE Q79FV7 NC_000962.3 Mycobrowser_v5 CDS 927837 930485 . - 0 Rv0834c PE_PGRS14 Function unknown PE-PGRS family protein PE_PGRS14 Rv0834c, (MTV043.26c), len: 882 aa. PE_PGRS14, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002), highly similar to many others e.g. MTCY493_4|Z95844 (1329 aa), FASTA scores: opt: 2577, E(): 0, (52.0% identity in 950 aa overlap); etc. Thought to be differentially expressed within host cells (see Triccas et al., 1999). Q79FV6 No PE/PPE Q79FV6 Q79FV6 Q79FV6 NC_000962.3 Mycobrowser_v5 CDS 930953 931597 . + 0 Rv0835 lpqQ Unknown Possible lipoprotein LpqQ Rv0835, (MTV043.27), len: 214 aa. Possible lpqQ, lipoprotein. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O53846 No cell wall and cell processes O53846 O53846 NC_000962.3 Mycobrowser_v5 CDS 932279 932932 . - 0 Rv0836c Rv0836c Unknown Hypothetical protein Rv0836c, (MTV043.29c), len: 217 aa (start uncertain). Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53848 No conserved hypotheticals O53848 O53848 NC_000962.3 Mycobrowser_v5 CDS 940456 941106 . - 0 Rv0844c narL Involved in transcriptional mechanism and regulates nitrate/nitrite. Possible nitrate/nitrite response transcriptional regulatory protein NarL Rv0844c, (MTV043.37c), len: 216 aa. Possible narL, nitrate/nitrite response regulator protein, similar to many e.g. CAB44989.1|AJ131854 NarL protein from Pseudomonas stutzeri (218 aa); CAA75536.1|Y15252 nitrate/nitrite regulatory protein from Pseudomonas aeruginosa (216 aa); PCC6803|D64005|SYCSLRG_24 NarL protein from Synechocystis sp. (209 aa), FASTA scores: opt: 438, E(): 1.5e-23, (34.6% identity in 208 aa overlap); etc. Also similar to unidentified regulator e.g. CAB76009.1|AL157916 putative two-component system response regulator from Streptomyces coelicolor (224 aa); etc. Contains probable helix-turn helix motif from aa 170-191 (Score 1124, +3.02 SD). P9WGM5 No regulatory proteins 3EUL O53856 P9WGM5 GO:0006355,GO:0043565,GO:0006350,GO:0003700,GO:0000156,GO:0000160,GO:0005622 P9WGM5 NC_000962.3 Mycobrowser_v5 CDS 941190 942467 . + 0 Rv0845 Rv0845 Possible sensor part of a two component regulatory system. Possible two component sensor kinase Rv0845, (MTV043.38), len: 425 aa. Possible two-component sensor kinase, with its C-terminus similar to C-terminal part of others e.g. NP_294951.1|NC_001263 two-component sensor histidine kinase from Deinococcus radiodurans (469 aa); CAC32293.1|AL583943 putative two component system histidine kinase from Streptomyces coelicolor (404 aa); NP_464546.1|NC_003210 protein similar to two-component sensor histidine kinase from Listeria monocytogenes (352 aa); BSUB0017_193|Z9912 two-component sensor kinase from Bacillus subtilis (360 aa), FASTA scores: opt: 275, E(): 1.6e-11, (30.3% identity in 234 aa overlap); etc. O53857 No regulatory proteins O53857 O53857 GO:0018106,GO:0007165,GO:0000155,GO:0005524 2.7.-.- O53857 NC_000962.3 Mycobrowser_v5 CDS 944938 946056 . + 0 Rv0848 cysK2 Thought to be involved in cysteine biosynthesis [catalytic activity: O3-acetyl-L-serine + H(2)S = L-cysteine + acetate]. Possible cysteine synthase a CysK2 (O-acetylserine sulfhydrylase) (O-acetylserine (thiol)-lyase) (CSASE) Rv0848, (MTV043.41), len: 372 aa. Possible cysK2, cysteine synthase A, but could be also a cysteine synthase B cysM2-product, similar to many e.g. NP_109408.1|NC_002682 cysteine synthase from Mesorhizobium loti (357 aa); Q44004|CYSM_ALCEU cysteine synthase from Alcaligenes eutrophus strain CH34 (Ralstonia eutropha) (339 aa), FASTA scores: opt: 511, E(): 1.7e-25, (35.0% identity in 314 aa overlap); etc. Belongs to the cysteine synthase/cystathionine beta-synthase family. Cofactor: pyridoxal phosphate. Note that previously known as cysM3. Q79FV4 No intermediary metabolism and respiration Q79FV4 Q79FV4 GO:0008152,GO:0030170,GO:0016740,GO:0004124 2.5.1.47 Q79FV4 NC_000962.3 Mycobrowser_v5 CDS 947641 948468 . - 0 Rv0851c Rv0851c Function unknown; possibly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv0851c, (MTV043.44c), len: 275 aa. Probable short-chain dehydrogenase/reductase, similar to many e.g. Q01198|LIGD_PSEPA C alpha-dehydrogenase (SDR family) from Pseudomonas paucimobilis (Sphingomonas paucimobilis) (305 aa); D11473|PSELIG_1 C alpha-dehydrogenase from P. paucimobilis (305 aa), FASTA scores: opt: 468, E(): 4.9e-23, (30.8% identity in 279 aa overlap); NP_421969.1|NC_002696 short chain dehydrogenase family protein from Caulobacter crescentus (278 aa); etc. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. O53863 No intermediary metabolism and respiration O53863 O53863 GO:0055114,GO:0016491 1.-.-.- O53863 NC_000962.3 Mycobrowser_v5 CDS 1025497 1026816 . - 0 Rv0920c Rv0920c Required for the transposition of the insertion element IS1554. Probable transposase Rv0920c, (MTCY21C12.14c), len: 439 aa. Probable transposase for IS1554, highly similar to others e.g. MTCY441.35|Q45111 transposase from Mycobacterium tuberculosis (419 aa), FASTA scores: opt: 1113, E(): 0, (43.9% identity in 378 aa overlap); etc. Contains transposases mutator family signature (PS01007). I6Y941 No insertion seqs and phages O05912 I6Y941 GO:0004803,GO:0006313,GO:0003677 I6Y941 NC_000962.3 Mycobrowser_v5 CDS 951632 952711 . + 0 Rv0855 far Function unknown, but involvement in lipid degradation (racemization). Probable fatty-acid-CoA racemase Far Rv0855, (MTV043.48), len: 359 aa. Probable far, fatty acid-CoA racemase, highly similar to CAB08122.1|Z94723 unknown protein from Mycobacterium leprae (253 aa) (C-terminus shorter). Also similar to many eukaryotic and bacteria racemases e.g. T35425 probable fatty acid CoA racemase from Streptomyces coelicolor (387 aa); P70473|AMAC_RAT alpha-methylacyl-CoA racemase (2-methylacyl-CoA racemase) (2-arylpropionyl-CoA epimerase) from Rattus norvegicus (Rat) (382 aa); NP_103687.1|NC_002678 probable fatty acid Co-a racemase from Mesorhizobium loti (389 aa); etc. Also similar to proteins from Mycobacterium tuberculosis e.g. Rv1143|MTCI65.10|MCR from Mycobacterium tuberculosis (360 aa), FASTA scores: opt: 1373, E(): 0, (56.8% identity in 359 aa overlap), Rv1866|MTCY359.07 (C-terminal half) (778 aa), Rv3272 (360 aa). I6Y8Y0 No lipid metabolism 2G04 O53867 I6Y8Y0 GO:0008152,GO:0016853 5.1.-.- I6Y8Y0 NC_000962.3 Mycobrowser_v5 CDS 955077 956288 . + 0 Rv0859 fadA Function unknown, but involvement in lipid degradation. Possible acyl-CoA thiolase FadA Rv0859, (MTV043.52), len: 403 aa. Possible fadA, acyl-CoA thiolase, equivalent to NP_302423.1|NC_002677 putative beta-ketoadipyl CoA thiolase from Mycobacterium leprae (403 aa). Also highly similar to acyl/acetyl-CoA thiolases and beta-ketoadipyl CoA thiolases, e.g. T35428 probable acetyl CoA acetyltransferase (thiolase) from Streptomyces coelicolor (404 aa); NP_250427.1|NC_002516 probable acyl-CoA thiolase from Pseudomonas aeruginosa (401 aa); NP_106253.1|NC_002678 probable acyl-CoA thiolase from Mesorhizobium loti (402 aa); NP_248919.1|NC_002516|PcaF beta-ketoadipyl CoA thiolase PcaF from Pseudomonas aeruginosa (401 aa); etc. Contains PS00098 Thiolases acyl-enzyme intermediate signature, PS00737 Thiolases signature 2 and PS00099 Thiolases active site. O53871 No lipid metabolism O53871 O53871 GO:0008152,GO:0008415 2.3.1.- O53871 NC_000962.3 Mycobrowser_v5 CDS 956293 958455 . + 0 Rv0860 fadB Involved in fatty acid degradation (probably in fatty acid beta-oxidation cycle). Probable fatty oxidation protein FadB Rv0860, (MTV043.53), len: 720 aa. Probable fadB, fatty oxidation protein, equivalent to NP_302422.1|NC_002677 putative fatty oxidation complex alpha subunit from Mycobacterium leprae (714 aa). Also highly similar to others and various proteins involved in fatty acid metabolism, e.g. T35429 probable fatty oxidation protein from Streptomyces coelicolor (733 aa); NP_250428.1|NC_002516 probable 3-hydroxyacyl-CoA dehydrogenase from Pseudomonas aeruginosa (714 aa); NP_418895.1|NC_002696 fatty oxidation complex alpha subunit from Caulobacter crescentus (709 aa); P40939|ECHA_HUMAN trifunctional enzyme alpha subunit [includes: long-chain enoyl-CoA hydratase ; long chain 3-hydroxyacyl-CoA dehydrogenase ] from Homo sapiens (763 aa), FASTA scores: opt: 1176, E(): 0, (32.4% identity in 722 aa overlap); P21177|FADB_ECOLI fatty oxidation complex alpha subunit [includes: enoyl-CoA hydratase; delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase; 3-hydroxyacyl-CoA dehydrogenase; 3- hydroxybutyryl-CoA epimerase] from Escherichia coli strain K12 (729 aa), FASTA scores: opt: 873, E(): 0, (33.6% identity in 693 aa overlap); etc. O53872 No lipid metabolism O53872 O53872 GO:0006631,GO:0016491,GO:0050662 O53872 NC_000962.3 Mycobrowser_v5 CDS 958523 960151 . - 0 Rv0861c ercc3 Involved in nucleotide excision repair. Has ATP-dependent 3' -> 5'helicase activity: acts by opening DNA either around the RNA transcription start site or the DNA damage. DNA helicase Ercc3 Rv0861c, (MTV043.54c), len: 542 aa. Ercc3, DNA helicase (see citation below), equivalent to NP_302420.1|NC_002677 probable DNA helicase from Mycobacterium leprae (549 aa). Also highly similar to others (shorter than several eukaryotic enzymes) e.g. NP_218820.1|NC_000919|AE001217|AE0 01217_6 putative DNA repair helicase from Treponema pallidum (606 aa), FASTA scores: opt: 1275, E(): 0, (47.5% identity in 592 aa overlap); Q00578|RA25_YEAST DNA repair helicase from Saccharomyces cerevisiae (843 aa), FASTA scores: opt: 777, E(): 0, (30.4% identity in 605 aa overlap); P49135|XPB_MOUSE DNA-repair protein complementing XP-B cells from Mus musculus (Mouse) (783 aa), FASTA scores: opt: 761, E(): 0, (36.3% identity in 375 aa overlap); etc. Seems to belong to the helicase family. Alternative nucleotide at position 958922 (C->a; A410A) has been observed. O53873 No information pathways O53873 O53873 GO:0004003,GO:0003677,GO:0006289,GO:0005524 3.6.1.- O53873 NC_000962.3 Mycobrowser_v5 CDS 962890 963393 . + 0 Rv0864 moaC2 Involved in the biosynthesis of molybdopterin. Probable molybdenum cofactor biosynthesis protein C 2 MoaC2 Rv0864, (MTV043.57), len: 167 aa. Probable moaC2, molybdopterin cofactor biosynthesis protein, highly similar to others e.g. CAB59676.1|AL132674 molybdenum cofactor biosynthesis protein from Streptomyces coelicolor (170 aa); NP_418834.1|NC_002696 molybdenum cofactor biosynthesis protein C from Caulobacter crescentus (186 aa); Y10817|ANY10817_3|T44852 molybdopterin co-factor synthesis protein moaC from Arthrobacter nicotinovorans plasmid pAO1 (169 aa), FASTA scores: opt: 491, E(): 2.4e-29, (51.0% identity in 151 aa overlap); etc. Also highly similar to O05788|MOAC1|Rv3111|MTCY164.21 putative molybdenum cofactor biosynthesis protein C from Mycobacterium tuberculosis (170 aa), FASTA scores: opt: 491, E(): 2.4e-29, (54.9% identity in 153 aa overlap); and O53376|Rv3324c|MOAC3|MTV016.24c putative molybdenum cofactor biosynthesis protein C3 (177 aa). P9WJR7 No intermediary metabolism and respiration P0A5K6 P9WJR7 GO:0006777 P9WJR7 NC_000962.3 Mycobrowser_v5 CDS 963390 963872 . + 0 Rv0865 mog Involved in molybdopterin biosynthesis; involved in the biosynthesis of a demolybdo-cofactor (molybdopterin), necessary for molybdo-enzymes. Probable molybdopterin biosynthesis Mog protein Rv0865, (MTV043.58), len: 160 aa. Probable mog, molybdopterin biosynthesis MOG protein, highly similar or similar to other molybdenum cofactor biosynthesis proteins e.g. CAB59675.1|AL132674 molybdenum cofactor biosynthesis protein from Streptomyces coelicolor (179 aa); NP_301253.1|NC_002677 putative molybdenum cofactor biosynthesis protein from Mycobacterium leprae (181 aa); CAC39235.1|AJ312124 Mog protein from Eubacterium acidaminophilum (162 aa); P44645|MOG_HAEIN|MOGA|HI0336 molybdopterin biosynthesis MOG protein from Haemophilus influenzae (197 aa), FASTA scores: opt: 306, E(): 9e-13, (39.6% identity in 139 aa overlap); P28694|MOG_ECOLI molybdopterin biosynthesis MOG protein from Escherichia coli (195 aa), FASTA scores: opt: 265, E(): 3.6e-10, (34.2 identity in 146 aa overlap); etc. Also highly similar to Rv0984|MTV044.12|MOAB2 possible pterin-4-alpha-carbinolamine dehydratase from Mycobacterium tuberculosis (181 aa). I6Y8Y8 No intermediary metabolism and respiration 2G4R O53877 I6Y8Y8 GO:0006777 I6Y8Y8 NC_000962.3 Mycobrowser_v5 CDS 963869 964294 . + 0 Rv0866 moaE2 Possibly a molybdenum biosynthesis cofactor. Conversion of molybdopterin precursor Z into molybdopterin requires transfer of two sulfur atoms to precursor Z (to generate the dithiolene group). This is catalyzed by the converting factor composed of a small and large subunit. Probable molybdenum cofactor biosynthesis protein E2 MoaE2 (molybdopterin converting factor large subunit) (molybdopterin [MPT] converting factor, subunit 2) Rv0866, (MTV043.59), len: 141 aa. Probable moaE2, molybdopterin converting factor E (molybdopterin converting factor (subunit 2)), similar to others e.g. Y10817|ANY10817_4|T44853 molybdopterin biosynthesis protein E chain from Arthrobacter nicotinovorans plasmid pAO1 (155 aa), FASTA scores: opt: 460, E(): 3.5e-27, (49.3 identity in 146 aa overlap); CAC01331.1|AL390968 moaE-like protein from Streptomyces coelicolor (152 aa); NP_389313.1|NC_000964 molybdopterin converting factor (subunit 2) from Bacillus subtilis (157 aa); etc. Also highly similar to Rv3119|MOAE1|Z95150|MTCY164_30 putative molybdenum cofactor biosynthesis protein E from Mycobacterium tuberculosis (147 aa), FASTA scores: opt: 321, E(): 5.9e-17, (40.9% identity in 132 aa overlap); and O53375|GPHA|Rv3323c|MTV016.23c MOAD-MOAE fusion protein from Mycobacterium tuberculosis (221 aa). P9WJR1 No intermediary metabolism and respiration O53878 P9WJR1 GO:0016740,GO:0006777 2.-.-.- P9WJR1 NC_000962.3 Mycobrowser_v5 CDS 966265 967347 . - 0 Rv0869c moaA2 Involved in molybdenum cofactor biosynthesis; involved in the biosynthesis of molybdopterin precursor Z from guanosine. Probable molybdenum cofactor biosynthesis protein A2 MoaA2 Rv0869c, (MTV043.62c), len: 360 aa. Probable moaA2, molybdenum cofactor biosynthesis protein, highly similar to others e.g. CAB59437.1|AL132644|SCI8_6 molybdenum cofactor biosynthesis protein A from Streptomyces coelicolor (341 aa), FASTA scores: opt: 1336, E(): 0, (61.7% identity in 332 aa overlap); S57490|X78980|ANMOAA_1 molybdopterin cofactor synthesis protein from Arthrobacter nicotinovorans (fragment) (374 aa), FASTA scores: opt: 1059, E(): 0, (49.9% identity in 369 aa overlap); Q44118|MOAA_ARTNI probable molybdopterin cofactor synthesis protein A from Arthrobacter nicotinovorans plasmid pAO1 (355 aa); etc. Also similar to Rv3109|MTCY164.19|Z95150|MOAA1 putative molybdenum cofactor biosynthesis protein A from Mycobacterium tuberculosis (359 aa), FASTA scores: opt: 657, E(): 0, (36.6% identity in 309 aa overlap). Belongs to the MoaA / NifB / PqqE family. P9WJS1 No intermediary metabolism and respiration P65384 P9WJS1 GO:0005525,GO:0006777,GO:0003824,GO:0046872,GO:0019008,GO:0051539 P9WJS1 NC_000962.3 Mycobrowser_v5 CDS 976872 978203 . - 0 Rv0878c PPE13 Function unknown PPE family protein PPE13 Rv0878c, (MTCY31.06c), len: 443 aa. PPE13, Member of the Mycobacterium tuberculosis PPE family, highly similar to many e.g. P4261|YHS6_MYCTU (517 aa), FASTA scores: opt: 1044, E(): 0, (47.4% identity in 397 aa overlap); MTV014_3, MTCI65_2, MTCY98_24, MTCY3C7_23, MTCY48_17, MTV004_5, MTV004_3, etc. Predicted to be an outer membrane protein (See Song et al., 2008). P9WI35 No PE/PPE Q10540 P9WI35 GO:0005886,GO:0016021 P9WI35 NC_000962.3 Mycobrowser_v5 CDS 979362 980228 . + 0 Rv0881 Rv0881 Causes methylation Possible rRNA methyltransferase (rRNA methylase) Rv0881, (MTCY31.09), len: 288 aa. Possible rRNA methyltransferase, highly similar to others and hypothetical proteins e.g. CAB76071.1|AL157953 putative rRNA methylase from Streptomyces coelicolor (272 aa); NP_421117.1|NC_002696 spoU rRNA methylase family protein from Caulobacter crescentus (268 aa); D90913_93|P74261 rRNA methylase from Synechocystis sp. (274 aa), FASTA scores: E(): 1.1e-13, (26.3% identity in 278 aa overlap); P18644|TSNR_STRCN rRNA methyltransferase from Streptomyces cyaneus (Streptomyces curacoi) (269 aa), FASTA scores: E(): 3.7e-08, (23.9% identity in 268 aa overlap); etc. Equivalent to AAK45146.1 from Mycobacterium tuberculosis strain CDC1551 (242 aa) but longer 46 aa. P9WFY3 No intermediary metabolism and respiration Q10543 P9WFY3 GO:0008173,GO:0006396,GO:0003723 2.1.1.- P9WFY3 NC_000962.3 Mycobrowser_v5 CDS 981424 982554 . - 0 Rv0884c serC Catalyzes the reversible interconversion of phosphoserine and 2-oxoglutarate to 3-phosphonooxypyruvate and glutamate. Require both in the major phosphorylated pathway of serine biosynthesis and in pyridoxine biosynthesis [catalytic activity: O-phospho-L-serine + 2-oxoglutarate = 3-phosphonooxypyruvate + L-glutamate]. Possible phosphoserine aminotransferase SerC (PSAT) Rv0884c, (MTCY31.12c), len: 376 aa. Possible serC, phosphoserine aminotransferase, equivalent to MLCB57_17 putative phosphoserine aminotransferase from Mycobacterium leprae (376 aa), FASTA scores: E(): 0, (87.5 identity in 376 aa overlap). Also highly similar to CAC08322.1|AL392149 putative aminotransferase from Streptomyces coelicolor (363 aa); and similar to other phosphoserine aminotransferases e.g. NP_386837.1|NC_003047 putative phosphoserine aminotransferase protein from Sinorhizobium meliloti (392 aa); P52878|SERC_METBA phosphoserine aminotransferase from Methanosarcina barkeri (370 aa); P10658|SERC_RABIT|RABEPIP_1 phosphoserine aminotransferase from Rabbit (370 aa), FASTA scores: opt: 271, E(): 3.5e-11, (24.5% identity in 368 aa overlap); etc. Belongs to class-V of pyridoxal-phosphate-dependent aminotransferases. Cofactor: pyridoxal phosphate. P9WQ73 No intermediary metabolism and respiration 2FYF P63514 P9WQ73 GO:0004648,GO:0005737,GO:0030170,GO:0008615,GO:0006564 2.6.1.52 P9WQ73 NC_000962.3 Mycobrowser_v5 CDS 988740 989861 . - 0 Rv0889c citA Involved in tricarboxylic acid cycle (KREBS cycle) [catalytic activity: citrate + CoA = acetyl-CoA + H2O + oxaloacetate]. Probable citrate synthase II CitA Rv0889c, (MTCY31.17c), len: 373 aa. Probable citA (alternate gene name: gltA), citrate synthase 2, highly similar to others e.g. CAB95899.1|AL359988 putative citrate synthase from Streptomyces coelicolor (387 aa); P39119|CISY_BACSU citrate synthase II from Bacillus subtilis (366 aa), FASTA scores: opt: 586, E(): 5.8e-30, (33.8% identity in 367 aa overlap); etc. Also similar to Rv0896|MTCY31.24 from Mycobacterium tuberculosis (29.2% identity in 274 aa overlap) and Rv1131. Contains PS00480 Citrate synthase signature. Belongs to the citrate synthase family. P9WPD3 No intermediary metabolism and respiration P63777 P9WPD3 GO:0004108,GO:0006099,GO:0044262 2.3.3.1 P9WPD3 NC_000962.3 Mycobrowser_v5 CDS 1002812 1003792 . + 0 Rv0899 ompA The protein behaved as a porin of low specific activity. Structural protein that may protect the integrity of the bacterium. Outer membrane protein A OmpA Rv0899, (MTCY31.27), len: 326 aa. OmpA, outer membrane protein A (See Senaratne et al., 1998). C-terminal region similar to C-terminus of many members of the OmpA family of outer membrane proteins, e.g. NP_458280.1|NC_003198 putative outer membrane protein from Salmonella enterica subsp. enterica serovar Typhi (220); NP_418008.1|NC_000913 putative outer membrane protein from Escherichia coli strain K12 (219 aa), FASTA scores: opt: 296, E(): 2.2e-11, (45.3% identity in 117 aa overlap); NP_231844.1|NC_002505 outer membrane protein OmpA from Vibrio cholerae (321 aa); Q05146|OMPA_BORAV outer membrane protein A precursor from Bordetella avium (194 aa); etc. A signal peptide sequence probably exists at the N-terminus. N-terminal domain is necessary and sufficient for membrane translocation (See Alahari et al., 2007). Contains PS00044 Bacterial regulatory proteins, lysR family signature. Belongs to the OmpA family. Pore-forming activity is pH-dependent. P9WIU5 No cell wall and cell processes 2KGS,2KGW,2KSM,2L26,2LBT,2LCA P65593 P9WIU5 GO:0016021,GO:0005886,GO:0009279 P9WIU5 NC_000962.3 Mycobrowser_v5 CDS 1004501 1005841 . - 0 Rv0902c prrB Sensor part of the two component regulatory system PRRA/PRRB. Thought to be involved in the environmental adaptation, specifically in an early phase of the intracellular growth. Two component sensor histidine kinase PrrB Rv0902c, (MTCY31.30c), len: 446 aa. PrrB, two-component sensor histidine kinase (see citations below), transmembrane protein, equivalent to MLCB57_26|NP_302403.1|NC_002677 sensor histidine kinase from Mycobacterium leprae (446 aa); and similar at C-termini to NP_301251.1|NC_002677 putative two-component system sensor kinase from Mycobacterium leprae (519 aa). C-terminus also similar to the C-termini of many sensor-like histidine kinase proteins e.g. P08336|CPXA_ECOLI|ECFB|SSD|EUP|B3911|Z5456|ECS4837 sensor protein from Escherichia coli strain K12 (457 aa), FASTA scores: opt: 364, E(): 1.7e-15, (27.1% identity in 398 aa overlap); CAB89748.1|AL354616 putative two-component histidine kinase from Streptomyces coelicolor (483 aa); CAB82845.1|AJ277081 putative histidine kinase from Amycolatopsis mediterranei (472 aa); etc. Also similar in part to Mycobacterium tuberculosis proteins Rv3764c (475 aa); and Rv0982 (504 aa). Thought to be induced at phagocytosis (see Graham & Clark-Curtiss 1999). P9WGK7 No regulatory proteins 1YS3,1YSR P0A5Z8 P9WGK7 GO:0016021,GO:0018106,GO:0005886,GO:0007165,GO:0000155,GO:0000160,GO:0005524 2.7.13.3 P9WGK7 NC_000962.3 Mycobrowser_v5 CDS 1006693 1008180 . - 0 Rv0904c accD3 This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA [catalytic activity ATP + acetyl-CoA + HCO(3)(-) = ADP + phosphate + malonyl-CoA]. Putative acetyl-coenzyme A carboxylase carboxyl transferase (subunit beta) AccD3 (accase beta chain) Rv0904c, (MTCY31.32c, MT0927), len: 495 aa. Putative accD3, acetyl-CoA carboxylase carboxyl transferase, beta subunit (carboxyltransferase subunit of acetyl-CoA carboxylase), highly similar in part to AAA63045.1|U15184 zinc finger protein from Mycobacterium leprae (201 aa). Also highly similar to others e.g. CAC42827.1|Y17592 putative carboxyltransferase subunit of acetyl-CoA carboxylase from Corynebacterium glutamicum (491 aa); CAB86110.1|AL163003 putative acetyl CoA carboxylase (alpha and beta subunits) from Streptomyces coelicolor (458 aa); Q54776|ACCD_SYNP7 acetyl-coenzyme A carboxylase carboxyl transferase subunit beta from Synechococcus sp. (305 aa); P12217|ACCD_MARPO acetyl-coenzyme A carboxylase carboxyl transferase subunit beta from Marchantia polymorpha (316 aa), FASTA scores: opt: 519, E():1.6e-24, (40.2% identity in 219 aa overlap); etc. Also similar to Rv3280, Rv2502c, etc from Mycobacterium tuberculosis. Belongs to the ACCD/PCCB family. P9WQH9 No lipid metabolism P63405 P9WQH9 GO:0003989,GO:0009317,GO:0006633,GO:0005524 6.4.1.2 P9WQH9 NC_000962.3 Mycobrowser_v5 CDS 1011731 1014124 . + 0 Rv0908 ctpE Metal cation-transporting ATPase; possibly catalyzes the transport of an undetermined metal cation with the hydrolysis of ATP [catalytic activity: ATP + H(2)O + undetermined metal cation(in) = ADP + phosphate + undetermined metal cation(out)]. Probable metal cation transporter ATPase P-type CtpE Rv0908, (MTCY21C12.02), len: 797 aa. Probable ctpE, metal cation-transporting ATPase P-type, transmembrane protein, E1-E2 family, highly similar to many e.g. AB93406.1|AL357524 putative integral membrane ATPase from Streptomyces coelicolor (802 aa); NP_346063.1|NC_003028 cation-transporting ATPase (E1-E2 family) from Streptococcus pneumoniae (778 aa); P37278|ATCL_SYNP7|PACL cation-transporting atpase from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (926 aa), FASTA scores: opt: 257, E(): 4.8e-33, (27.7% identity in 905 aa overlap); etc. Contains E1-E2 ATPases phosphorylation site (PS00154). Belongs to the cation transport ATPases family (E1-E2 ATPases). P9WPT1 No cell wall and cell processes P0A504 P9WPT1 GO:0006754,GO:0015662,GO:0016021,GO:0046872,GO:0005886,GO:0005524 3.6.3.- P9WPT1 NC_000962.3 Mycobrowser_v5 CDS 1018727 1019965 . - 0 Rv0914c Rv0914c Thought to be involved in degradative pathways such as fatty acid BETA_OXIDATION. Possible lipid carrier protein or keto acyl-CoA thiolase Rv0914c, (MTCY21C12.08c), len: 412 aa. Possible lipid carrier protein or keto acyl-CoA thiolase, highly similar to NP_421905.1|NC_002696 thiolase family protein from Caulobacter crescentus (407 aa); and similar to others e.g. NP_107896.1|NC_002678 3-ketoacyl-CoA thiolase from Mesorhizobium loti (392 aa); NP_385796.1|NC_003047 putative 3-ketoacyl-CoA thiolase protein from Sinorhizobium meliloti (389 aa); NP_275932.1|NC_000916 lipid-transfer protein (sterol or nonspecific) from Methanothermobacter thermautotrophicus (383 aa); AB55378.1|AL117263 possible 3-ketoacyl-CoA thiolase from Leishmania major (441 aa), FASTA scores: opt: 547, E(): 3.1e-26, (31.0% identity in 435 aa overlap); etc. Also similar to Rv2790c, Rv1627c, Rv0244, etc from Mycobacterium tuberculosis. Could belong to the thiolase family. I6XWJ8 No lipid metabolism O05906 I6XWJ8 GO:0008152,GO:0003988 2.3.1.16 I6XWJ8 NC_000962.3 Mycobrowser_v5 CDS 1022087 1023868 . + 0 Rv0917 betP High-affinity uptake of glycine betaine. Supposedly responsible for the translocation of the substrate across the membrane. Possible glycine betaine transport integral membrane protein BetP Rv0917, (MTCY21C12.11), len: 593 aa. Possible betP, glycine betaine transporter, integral membrane protein, highly similar to many transporters, mainly glycine betaine transporters, e.g. P54582|BETP_CORGL glycine betaine transporter from Corynebacterium glutamicum (Brevibacterium flavum) (595 aa), FASTA scores: opt: 1367, E(): 0, (42.7% identity in 504 aa overlap); T35264 probable BccT family transporter from Streptomyces coelicolor (578 aa); NP_243511.1|NC_002570 glycine betaine transporter from Bacillus halodurans (504 aa); NP_439848.1|NC_000907 high-affinity choline transport protein (betT) from Haemophilus influenzae (669 aa); etc. Seems to belong to the BCCT (TC 2.33) family of transporters. P9WPR7 No cell wall and cell processes P63695 P9WPR7 GO:0005886,GO:0006810,GO:0005215,GO:0016021 P9WPR7 NC_000962.3 Mycobrowser_v5 CDS 1030578 1031864 . - 0 Rv0924c mntH H(+)-stimulated, highly selective, divalent cation uptake system. Responsible for the translocation of the divalent metal across the membrane. Divalent cation-transport integral membrane protein MntH (BRAMP) (MRAMP) Rv0924c, (MTCY21C12.18c), len: 428 aa. MntH (alternative gene name: Nramp, Mramp), H+-dependent divalent cation-transport integral membrane protein (see citations below), equivalent to O69443|MNTH_MYCBO probable manganese transport protein MNTH (BRAMP) from Mycobacterium bovis (415 aa); and NP_302396.1|NC_002677 probable manganese transport protein from Mycobacterium leprae (426 aa). Also similar (but longer 51 aa in N-terminus) to AAA63075.1|U15184 SMF2 protein from Mycobacterium leprae (377 aa), FASTA scores: opt: 1780, E(): 0, (74.5% identity in 376 aa overlap). Also similar to many orthologues of the eukaryotic Nramp (natural resistance-associated macrophage protein), also known as mntH, e.g. NP_456951.1|NC_003198 manganese transport protein MntH from Salmonella enterica subsp. enterica serovar Typhi (413 aa); etc. Belongs to the NRAMP family. P9WIZ5 No cell wall and cell processes O05916 P9WIZ5 GO:0030145,GO:0006828,GO:0005886,GO:0015293,GO:0016021 P9WIZ5 NC_000962.3 Mycobrowser_v5 CDS 1036028 1037002 . + 0 Rv0929 pstC2 Involved in active transport of inorganic phosphate across the membrane (import); responsible for the translocation of the substrate across the membrane. This is one of the proteins required for binding-protein-mediated phosphate transport. Phosphate-transport integral membrane ABC transporter PstC2 Rv0929, (MTCY21C12.23), len: 324 aa. PstC2, phosphate-transport integral membrane ABC transporter (see citations below), highly similar to others e.g. NP_302394.1|NC_002677 membrane-bound component of phosphate transport from Mycobacterium leprae (319 aa); CAB88474.1|AL353816 phosphate ABC transport system permease protein from Streptomyces coelicolor (336 aa); NP_290359.1| NC_002655 high-affinity phosphate-specific transport system (cytoplasmic membrane component) from Escherichia coli strain O157:H7 (319 aa); etc. Also similar to Rv935|MTCY08D9.04c|PSTC1 probable transmembrane ABC transporter component of phosphate uptake system from Mycobacterium tuberculosis (338 aa). Contains binding-protein-dependent transport systems inner membrane component signature (PS00402). P9WG05 No cell wall and cell processes P0A630 P9WG05 GO:0016021,GO:0006817,GO:0005886,GO:0005315 P9WG05 NC_000962.3 Mycobrowser_v5 CDS 1039936 1041048 . - 0 Rv0932c pstS2 Involved in active transport of inorganic phosphate across the membrane (import). This is one of the proteins required for binding-protein-mediated phosphate transport. Periplasmic phosphate-binding lipoprotein PstS2 (PBP-2) (PstS2) Rv0932c, (MTCY08D9.07), len: 370 aa. PstS2, phosphate-binding lipoprotein component of inorganic phosphate transport system (see citations below), highly similar to AAF74819.1|AF137360_1|AF137360 periplasmic phosphate permease from Mycobacterium avium (369 aa); Rv0928|MTCY21C12.22|pstS3 phosphate-binding periplasmic lipoprotein from Mycobacterium tuberculosis (370 aa), FASTA scores: opt: 1601, E(): 0, (64.5% identity in 372 aa overlap); and Rv0934|MTCY08D9.05c|pstS1 phosphate-binding periplasmic lipoprotein from Mycobacterium tuberculosis (374 aa) (Mycobacterium tuberculosis seems to have three PstS-like proteins, others being Rv0928 and Rv0934c). Also highly similar to MTCY08D9.05c|P15712|PAB_MYCTU protein antigen B precursor from Mycobacterium tuberculosis (374 aa), FASTA scores: opt: 460, E(): 2.7e-20, (31.2% identity in 375 aa overlap). Contains prokaryotic membrane lipoprotein lipid attachment site (PS00013) at N-terminus so the leader peptide of 22 aa is probably removed. Belongs to family of phosphate receptors for bacterial ABC-type lipoprotein transporters. Appears to be co-transcribed with Rv0931c|pknD|mbk. P9WGT9 No cell wall and cell processes O05870 P9WGT9 GO:0006817,GO:0005886,GO:0005315 P9WGT9 NC_000962.3 Mycobrowser_v5 CDS 1042115 1043239 . + 0 Rv0934 pstS1 Involved in active transport of inorganic phosphate across the membrane (import). This is one of the proteins required for binding-protein-mediated phosphate transport. Periplasmic phosphate-binding lipoprotein PstS1 (PBP-1) (PstS1) Rv0934, (MTCY08D9.05c), len: 374 aa. PstS1 (previously known as phoS1 or phoS), phosphate-binding lipoprotein component of inorganic phosphate transport system (see citations below), highly similar to Rv0932c|MTCY08D9.07|pstS2 phosphate-binding periplasmic lipoprotein from Mycobacterium tuberculosis (370 aa), FASTA scores: opt: 460, E(): 5.9e-19, (31.2% identity in 375 aa overlap); and Rv0928|MTCY21C12.22|pstS3 phosphate-binding periplasmic lipoprotein from Mycobacterium tuberculosis (374 aa), FASTA scores: opt: 435, E():1.1e-17, (30.0% identity in 380 aa overlap) (Mycobacterium tuberculosis seems to have three PstS-like proteins, others being Rv0932c and Rv0928c). Also highly similar to MTCY08D9.05c|P15712|PAB_MYCTU protein antigen B precursor from Mycobacterium tuberculosis (374 aa), FASTA scores: opt: 2459, E(): 0, (100% identity in 374 aa overlap). Contains a prokaryotic membrane lipoprotein lipid attachment site (PS00013) at the N-terminus so the 23 aa leader peptide sequence is probably removed. Belongs to family of phosphate receptors for bacterial ABC-type lipoprotein transporters. P9WGU1 No cell wall and cell processes 1PC3 P15712 P9WGU1 GO:0006817,GO:0005886,GO:0005315 P9WGU1 NC_000962.3 Mycobrowser_v5 CDS 1046136 1048415 . + 0 Rv0938 ligD Involved in DNA double-strand break repair, by nonhomologous end joining (NHEJ). Interacts with KU (Rv0937c) [catalytic activity:ATP + {deoxyribonucleotide}(N) + {deoxyribonucleotide}(M) = AMP + diphosphate + {deoxyribonucleotide}(N+M)]. ATP dependent DNA ligase LigD (ATP dependent polydeoxyribonucleotide synthase) (thermostable DNA ligase) (ATP dependent polynucleotide ligase) (sealase) (DNA repair enzyme) (DNA joinase) Rv0938, (MTCY08D9.01c, MTCY10D7.36c), len: 759 aa. ligD, ATP-dependent DNA ligase, with its C-terminus similar to N-terminal parts of many ATP-dependent DNA ligases e.g. NP_250828.1|NC_002516 probable ATP-dependent DNA ligase from Pseudomonas aeruginosa (840 aa); NP_105436.1|NC_002678 ATP-dependent DNA ligase from Mesorhizobium loti (829 aa); CAB92891.1|AL356932 probable ATP-dependent DNA ligase from Streptomyces coelicolor (326 aa); etc. The N-terminal half shows similarity with hypothetical proteins from Mycobacterium tuberculosis Rv0269c and Rv3730c; and the C-terminal half with the DNA ligases Rv3731 and Rv3062. P9WNV3 No information pathways 1VS0,2IRU,2IRX,2IRY,2R9L,3PKY P71571 P9WNV3 GO:0003910,GO:0003896,GO:0006310,GO:0006281,GO:0006269,GO:0005524 P9WNV3 NC_000962.3 Mycobrowser_v5 CDS 1054247 1055008 . + 0 Rv0945 Rv0945 Function unknown; possibly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv0945, (MTCY10D7.29c), len: 253 aa. Probable short-chain dehydrogenase/reductase, similar to various dehydrogenases/reductases e.g. NP_346338.1|NC_003028 oxidoreductase (short chain dehydrogenase/reductase family) from Streptococcus pneumoniae (253 aa); AAB70845.1|AF019986|PksB from Dictyostelium discoideum (260 aa); AAF86624.1|U87786 clavaldehyde dehydrogenase from Streptomyces clavuligerus (247 aa); P37440|UCPA_ECOLI oxidoreductase from Escherichia coli (285 aa), FASTA scores: opt: 275, E(): 1.1e-12, (33.8% identity in 201 aa overlap); etc. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. P9WGR7 No intermediary metabolism and respiration P71564 P9WGR7 GO:0055114,GO:0016491 1.-.-.- P9WGR7 NC_000962.3 Mycobrowser_v5 CDS 1055024 1056685 . - 0 Rv0946c pgi Involved in glycolysis and in gluconeogenesis [catalytic activity: D-glucose 6-phosphate = D-fructose 6-phosphate]. Probable glucose-6-phosphate isomerase Pgi (GPI) (phosphoglucose isomerase) (phosphohexose isomerase) (phi) Rv0946c, (MTCY10D7.28), len: 553 aa. Probable pgi, glucose-6-phosphate isomerase, equivalent to NP_301236.1|NC_002677 glucose-6-phosphate isomerase from Mycobacterium leprae (554 aa); and P96803|G6PI_MYCSM glucose-6-phosphate isomerase from Mycobacterium smegmatis (442 aa). Also highly similar to others e.g. T36015 glucose-6-phosphate isomerase from Streptomyces coelicolor (551 aa); P11537|G6PI_ECOLI|GPI glucose-6-phosphate isomerase from Escherichia coli strains K12 and O157:H7 (549 aa), FASTA scores: opt: 1779, E(): 0, (51.4% identity in 554 aa overlap); etc. Contains PS00765 Phosphoglucose isomerase signature 1, and PS00174 Phosphoglucose isomerase signature 2. Belongs to the GPI family. P9WN69 No intermediary metabolism and respiration 2WU8 P64192 P9WN69 GO:0006094,GO:0004347,GO:0006096,GO:0005737 5.3.1.9 P9WN69 NC_000962.3 Mycobrowser_v5 CDS 1058260 1060575 . + 0 Rv0949 uvrD1 Involved in nucleotide excision repair. Has a 3'-5' helicase activity in presence of ATP. Preferred substrate being one with both single and double stranded regions of DNA. Probable ATP-dependent DNA helicase II UvrD1 Rv0949, (MTCY10D7.25c), len: 771 aa. Probable uvrD1, ATP dependent DNA helicase II (see citation below), equivalent to P_301239.1|NC_002677 putative ATP-dependent DNA helicase from Mycobacterium leprae (778 aa). Also highly similar to others e.g. CAB92660.1|AL356832 from Streptomyces coelicolor (831 aa) (N-terminus longer); P56255|PCRA_BACST from Bacillus stearothermophilus (724 aa); Q10213|YAY5_SCHPO from Schizosaccharomyces pombe (Fission yeast) (887 aa), FASTA scores: opt: 927, E(): 0, (33.5% identity in 659 aa overlap); etc. Also similar to several other UvrD-like proteins in Mycobacterium tuberculosis e.g. Rv3201c, Rv3198c, Rv3202c. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the UVRD subfamily of helicases. Note that previously known as uvrD. P9WMQ1 No information pathways P0A5A3 P9WMQ1 GO:0004003,GO:0003677,GO:0006281,GO:0006268,GO:0005737,GO:0005524 3.6.4.12 P9WMQ1 NC_000962.3 Mycobrowser_v5 CDS 1061964 1063127 . + 0 Rv0951 sucC Involved in tricarboxylic acid cycle [catalytic activity: ATP + succinate + CoA = ADP + succinyl-CoA + phosphate]. Probable succinyl-CoA synthetase (beta chain) SucC (SCS-beta) Rv0951, (MTCY10D7.23c), len: 387 aa. Probable sucC, succinyl-CoA synthetase, beta chain, equivalent to AL035500|MLCL373_3|NP_301241.1|NC_002677 succinyl-CoA synthase [beta] chain from Mycobacterium leprae (393 aa), FASTA score: (86.7% identity in 391 aa overlap). Also highly similar to others e.g. AB92671.1|AL356832 succinyl-CoA synthetase beta chain from Streptomyces coelicolor (394 aa); P25126|SUCC_THEFL succinyl-CoA synthetase beta chain from Thermus aquaticus (378 aa); P07460|SUCC_ECOLI succinyl-CoA synthetase beta chain from Escherichia coli (388 aa), FASTA scores: opt: 933, E(): 0, (41.0% identity in 390 aa overlap); etc. P9WGC5 No intermediary metabolism and respiration P71559 P9WGC5 GO:0046872,GO:0004775,GO:0006099,GO:0005524 6.2.1.5 P9WGC5 NC_000962.3 Mycobrowser_v5 CDS 1067561 1068208 . + 0 Rv0956 purN Involved in de novo purine biosynthesis (at the third step) [catalytic activity: 10-formyltetrahydrofolate + 5'-phosphoribosylglycinamide = tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide]. Probable 5'-phosphoribosylglycinamide formyltransferase PurN (GART) (gar transformylase) (5'-phosphoribosylglycinamide transformylase) Rv0956, (MTCY10D7.18c), len: 215 aa. Probable purN, 5'-phosphoribosylglycinamide formyltransferase, equivalent to AAF05726.1|AF191543_1|AF191543|PurN phosphoribosylglycinamide formyltransferase from Mycobacterium avium subsp. paratuberculosis (209 aa); and AL035500|MLCL373_7 from Mycobacterium leprae (215 aa), FASTA score: (79.4% identity in 214 aa overlap). Also highly similar to others e.g. BAA89443.1|AB003159 from Corynebacterium ammoniagenes (199 aa); NP_241498.1|NC_002570 from Bacillus halodurans (188 aa); P08179|PUR3_ECOLI|B2500 from Escherichia coli strain K12 (212 aa), FASTA scores: opt: 380, E(): 2.4e-18, (36.6% identity in 183 aa overlap); C-terminus of P16340|PUR2_DROPS trifunctional purine biosynthetic protein adenosine-3 from Drosophila pseudoobscura (Fruit fly) (1364 aa); etc. P9WHM5 No intermediary metabolism and respiration 3DA8,3DCJ P71554 P9WHM5 GO:0004644,GO:0006189 2.1.2.2 P9WHM5 NC_000962.3 Mycobrowser_v5 CDS 1068205 1069776 . + 0 Rv0957 purH Involved in de novo purine biosynthesis (at the ninth and tenth steps) [catalytic activity 1: 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-4-imidazolecarboxamide = tetrahydrofolate + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide] [catalytic activity 2: imp + H2O = 5-formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide]. Probable bifunctional purine biosynthesis protein PurH: phosphoribosylaminoimidazolecarboxamide formyltransferase (AICAR transformylase) (5'-phosphoribosyl-5-aminoimidazole-4-carboxamide formyltransferase) + inosinemonophosphate cyclohydrolase (imp cyclohydrolase) (inosinicase) (imp synthetase) (ATIC) Rv0957, (MTCY10D7.17c), len: 523 aa. Probable purH, bifunctional purine biosynthesis protein including 5'-phosphoribosyl-5-aminoimidazole-4-carboxamide formyltransferase and inosine-monophosphate (imp) cyclohydrolase, equivalent to AL035500|MLCL373_8 putative phosphoribosylaminoimidazolecarboxamide formyltransferase from Mycobacterium leprae (527 aa), FASTA score: (88.1% identity in 520 aa overlap); and AF05727.1|AF191543_2|AF191543|PurH from Mycobacterium avium subsp. paratuberculosis (527 aa). Also highly similar to others e.g. CAB92677.1|AL356832 bifunctional purine biosynthesis protein from Streptomyces coelicolor (523 aa); NP_388534.1|NC_000964 phosphoribosylaminoimidazole carboxy formyl formyltransferase + inosine-monophosphate cyclohydrolase from Bacillus subtilis (512 aa); P15639|PUR9_ECOLI phosphoribosylaminoimidazolecarboxamide formyltransferase from Escherichia coli (529 aa), FASTA scores: opt: 1147, E(): 0, (44.8% identity in 533 aa overlap); etc. Belongs to the PurH family. P9WHM7 No intermediary metabolism and respiration 3ZZM,4A1O P67541 P9WHM7 GO:0003937,GO:0004643,GO:0006188 3.5.4.10,2.1.2.3 P9WHM7 NC_000962.3 Mycobrowser_v5 CDS 1606386 1607972 . + 0 Rv1430 PE16 Function unknown PE family protein PE16 Rv1430, (MTCY493.24c), len: 528 aa. PE16, Member of the Mycobacterium tuberculosis PE family of proteins (see citation below), e.g. Y0D4_MYCTU|Q50594 (55.9% identity in 127 aa overlap). The C-terminus shows similarity to Q49633|LEPB1170_F3_112 hypothetical Mycobacterium leprae protein (391 aa), FASTA scores: opt: 342, E(): 1.2e-13, (29.8% identity in 292 aa overlap). Possible TMhelix aa 500-522. L7N697 No PE/PPE Q7D8G5 L7N697 L7N697 NC_000962.3 Mycobrowser_v5 CDS 1078743 1081055 . + 0 Rv0969 ctpV Metal cation-transporting ATPase; possibly catalyzes the transport of an undetermined metal cation with the hydrolysis of ATP [catalytic activity: ATP + H(2)O + undetermined metal cation(in) = ADP + phosphate + undetermined metal cation(out)]. Probable metal cation transporter P-type ATPase CtpV Rv0969, (MTCY10D7.05c), len: 770 aa. Probable ctpV, metal cation transporter P-type ATPase (transmembrane protein) (see citation below), part of cso operon, highly similar (except in N-terminus) to others e.g. NP_391230.1|NC_000964 similar to heavy metal-transporting ATPase from Bacillus subtilis (803 aa); P37279|ATCS_SYNP7|PACS cation-transporting ATPase from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (747 aa), FASTA scores: opt: 1851, E(): 0, (52.1% identity in 664 aa overlap); etc. Equivalent to AAK45246.1 from Mycobacterium tuberculosis strain CDC1551 (792 aa) but shorter 22 aa. Contains PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases). P9WPS3 No cell wall and cell processes P77894 P9WPS3 GO:0006754,GO:0004008,GO:0016021,GO:0046872,GO:0030001,GO:0005886,GO:0005524 3.6.3.- P9WPS3 NC_000962.3 Mycobrowser_v5 CDS 1179396 1180577 . + 0 Rv1057 Rv1057 Function unknown Conserved hypothetical protein Rv1057, (MTV017.10), len: 393 aa. Conserved hypothetical protein, some similarity to X84710|MMSAG_1 surface antigen of Methanosarcina mazeii (491 aa), FASTA scores: opt: 363, E():6.2e-15, (31.3% identity in 294 aa overlap). Regulated by MprA (Rv0981) under physiological conditions and environmental stress (SDS and Triton X-100) (See He et al., 2006). O53405 No conserved hypotheticals O53405 O53405 NC_000962.3 Mycobrowser_v5 CDS 1783309 1783623 . - 0 Rv1579c Rv1579c Unknown Probable PhiRv1 phage protein Rv1579c, (MTCY336.25), len: 104 aa. Probable phiRv1 phage protein (see citation below). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06611 No insertion seqs and phages O06611 NC_000962.3 Mycobrowser_v5 CDS 1083747 1085750 . - 0 Rv0973c accA2 This protein carries two functions: biotin carboxyl carrier protein and biotin carboxyltransferase. Involved in the first step of long-chain fatty acid synthesis [catalytic activity: ATP + biotin-carboxyl-carrier protein + CO(2) = ADP + phosphate + carboxybiotin-carboxyl-carrier protein]. Probable acetyl-/propionyl-coenzyme A carboxylase alpha chain (alpha subunit) AccA2: biotin carboxylase + biotin carboxyl carrier protein (BCCP) Rv0973c, (MTV044.01c, MTCY10D7.01), len: 667 aa. Probable accA2 (alternate gene name: bccA), acetyl-/propionyl-coenzyme A carboxylase (alpha subunit) [includes: biotin carboxylase ; biotin carboxyl carrier protein (BCCP)], highly similar to others e.g. CAB95892.1|AL359988 putative acetyl/propionyl CoA carboxylase alpha subunit from Streptomyces coelicolor (614 aa); NP_250702.1|NC_002516 probable acyl-CoA carboxylase alpha chain from Pseudomonas aeruginosa (655 aa); NP_420971.1|NC_002696 acetyl/propionyl-CoA carboxylase alpha subunit from Caulobacter crescentus ( 654 aa); NP_251581.1|NC_002516 probable biotin carboxylase/biotin carboxyl carrier protein from Pseudomonas aeruginosa (661 aa); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. Rv2501c|P46401|MTCY07A7.07c|BCCA_MYCTU|ACCA1 probable acetyl-/propionyl-coenzyme A carboxylase alpha chain (alpha subunit) (654 aa), FASTA scores, opt: 250, E(): 4e-09, (28.6% identity in 182 aa overlap); and Rv3285|MTCY71.25|ACCA3 (600 aa); Z83018|MTCY349_20 (1127 aa), FASTA scores: opt: 838, E(): 0, (40.2% identity in 500 aa overlap). Contains PS00867 Carbamoyl-phosphate synthase subdomain signature 2 and PS00188 Biotin-requiring enzymes attachment site. P71538 No lipid metabolism P71538 P71538 GO:0009374,GO:0004075,GO:0008152,GO:0005524 6.3.4.14 P71538 NC_000962.3 Mycobrowser_v5 CDS 1087348 1088496 . - 0 Rv0975c fadE13 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE13 Rv0975c, (MTV044.03c), len: 382 aa. Probable fadE13, acyl-CoA dehydrogenase, highly similar to many e.g. T35427 probable acyl-CoA dehydrogenase from Streptomyces coelicolor (382 aa); M74096|HUMACADL_1 Human long chain acyl-CoA dehydrogenase from Homo sapiens (430 aa), FASTA scores: opt: 819, E(): 0, (37.0% identity in 376 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. fadE20|Z98209|MTCY154_4 (386 aa), FASTA scores: (40.3% identity in 375 aa overlap). Contains PS00073 Acyl-CoA dehydrogenases signature 2. Belongs to the acyl-CoA dehydrogenases family. O86319 No lipid metabolism O86319 O86319 GO:0003995,GO:0055114,GO:0050660 1.3.99.- O86319 NC_000962.3 Mycobrowser_v5 CDS 1090373 1093144 . + 0 Rv0977 PE_PGRS16 Function unknown PE-PGRS family protein PE_PGRS16 Rv0977, (MTV044.05), len: 923 aa. PE_PGRS16, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below), highly similar to other PGRS-type sequences e.g. AL0091|MTV004_1 from Mycobacterium tuberculosis (1125 aa), FASTA score: (45.4% identity in 959 aa overlap); Z80225|MTCY441_4 from Mycobacterium tuberculosis (778 aa), FASTA score: (51.5% identity in 750 aa overlap); etc. Q79FU3 No PE/PPE Q79FU3 Q79FU3 Q79FU3 NC_000962.3 Mycobrowser_v5 CDS 1096822 1097508 . + 0 Rv0981 mprA Regulator part of a two component regulatory system (MPRAB system) Mycobacterial persistence regulator MRPA (two component response transcriptional regulatory protein) Rv0981, (MTV044.09), len: 228 aa. MprA, mycobacterial persistence regulator, a two-component response regulator whose expression is required for entrance into and maintenance of persistent infection (see citation below), equivalent to NP_301250.1|NC_002677 putative two-component response regulator from Mycobacterium leprae (228 aa); and highly similar to others from Mycobacterium leprae. Also highly similar to others e.g. AAG36759.1|AF119221_1|AF119221 response regulator from Corynebacterium glutamicum (232 aa); CAB88489.1|AL353816 putative two-component system response regulator from Streptomyces coelicolor (248 aa); BJY09666_1 two-component response regulator (ragA, ragB and rpoH3) from B.japonicum (226 aa), FASTA score: (43.8% identity in 224 aa overlap); BSAJ2571_44 two-component response regulator from Bacillus subtilis (228 aa), FASTA score: (46.4% identity in 224 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. Rv1033c (257 aa); Rv0903c (236 aa), FASTA score: (50.7 identity in 225 aa overlap); etc. Contains PS00217 Sugar transport proteins signature 2. Start changed since first submission (-2 aa). MprAB is involved in the regulation of genes in response to environmental stress (See He et al., 2006). P9WGM9 No regulatory proteins O53894 P9WGM9 GO:0006950,GO:0005737,GO:0009405,GO:0006355,GO:0006350,GO:0000156,GO:0000160,GO:0003677 P9WGM9 NC_000962.3 Mycobrowser_v5 CDS 1097508 1099022 . + 0 Rv0982 mprB Sensor part of a two component regulatory system (MPRAB system) Two component sensor kinase MprB Rv0982, (MTV044.10), len: 504 aa. MprB, two component sensor kinase, probable transmembrane protein (see citation below), equivalent to AL035500|MLCL373_16|NP_301251.1|NC_002677 putative two-component system sensor kinase from Mycobacterium leprae (519 aa), FASTA score: (81.0% identity in 521 aa overlap). Also highly similar to others (especially in C-terminal part) e.g. AAG36760.1|AF119221_2|AF119221 sensor kinase from Corynebacterium glutamicum (455 aa); CAB89748.1|AL354616 putative two-component histidine kinase from Streptomyces coelicolor (481 aa); X58793|SLCUTRS_2 sensor kinase from S.lividans (414 aa), FASTA scores: opt: 451, E(): 4.2e-21, (36.0% identity in 303 aa overlap); P30847|BAES_ECOLI sensor protein from Escherichia coli (467 aa), FASTA scores: opt: 412, E(): 1.3e-18, (30.4% identity in 336 aa overlap); etc. Also similar in C-terminal region to C-terminus of Rv0902c|Z73101|MTCY31_33 from Mycobacterium tuberculosis (446 aa), FASTA scores: opt: 423, E(): 2.6e-19, (28.4 identity in 462 aa overlap). MprAB is involved in the regulation of genes in response to environmental stress (See He et al., 2006). P9WGL1 No regulatory proteins O53895 P9WGL1 GO:0016021,GO:0009405,GO:0018106,GO:0004721,GO:0005886,GO:0006950,GO:0007165,GO:0000155,GO:0000160,GO:0005524 3.1.3.-,2.7.13.3 P9WGL1 NC_000962.3 Mycobrowser_v5 CDS 1101025 1101480 . - 0 Rv0985c mscL Ion channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell. Possible large-conductance ion mechanosensitive channel MscL Rv0985c, (MTV044.13c), len: 151 aa. Possible mscL, large conductance mechanosensitive ion channel (integral membrane protein) (see citations below, equivalent to AL035500|MLCL373_19|NP_301254.1|NC_002677 putative mechanosensitive channel protein from Mycobacterium leprae (154 aa), FASTA score: (71.0% identity in 155 aa overlap). Also highly similar to others e.g. NP_268999.1|NC_002737 putative large conductance mechanosensitive channel from Streptococcus pyogenes (120 aa); CAB90974.1|AL355832 putative mechanosensitive channel from Streptomyces coelicolor (156 aa); Q9X722|MSCL_CLOHI large-conductance mechanosensitive channel from Clostridium histolyticum (133 aa); Z83337|BSZ83337_6 large conductance mechanosensitive channel from Bacillus subtilis (130 aa), FASTA scores: opt: 248, E(): 8.4e-10, (39.0% identity in 136 aa overlap); U08371|ECU08371_1 large conductance mechanosensitive channel from Escherichia coli strain K-12 (136 aa), FASTA score: (36.6% identity in 134 aa overlap); etc. Belongs to the MscL family. P9WJN5 No cell wall and cell processes 2OAR P0A5K8 P9WJN5 GO:0005216,GO:0006811,GO:0005886,GO:0016021 P9WJN5 NC_000962.3 Mycobrowser_v5 CDS 1101803 1102549 . + 0 Rv0986 Rv0986 Thought to be involved in active transport of adhesion component across the membrane: involved in attachment and virulence. Responsible for energy coupling to the transport system. Probable adhesion component transport ATP-binding protein ABC transporter Rv0986, (MTV044.14), len: 248 aa. Probable ATP-binding protein ABC transporter supposedly involved in transport of adhesion component (see citation below), highly similar to many ATP-binding proteins e.g. AE0010|AE001033_8 ABC transporter ATP-binding protein from Archaeoglobus fulgidus (228 aa), FASTA scores: opt: 669, E(): 0, (45.7% identity in 219 aa overlap); CAB81857.1|AL161691 putative ABC-transporter ATP-binding protein from Streptomyces coelicolor (246 aa); X84019|ZMDNAGRP_4 glutamate uptake regulatory protein (grp) from Z.mobilis (232 aa), FASTA score: (44.4% identity in 225 aa overlap); Z99111|BSUB0008_108 from Bacillus subtilis (230 aa), FASTA score: (38.7% identity in 222 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). Believed to have been acquired by horizontal gene transfer (See Rosas-Magallanes et el., 2006; Becq et al., 2007). P9WQK1 No cell wall and cell processes O53899 P9WQK1 GO:0016887,GO:0006810,GO:0005524 P9WQK1 NC_000962.3 Mycobrowser_v5 CDS 1106405 1107382 . - 0 Rv0989c grcC2 Possible supplier of polyprenyl diphosphate. Probable polyprenyl-diphosphate synthase GrcC2 (polyprenyl pyrophosphate synthetase) Rv0989c, (MTCI237.03c), len: 325 aa. Probable grcC2, polyprenyl diphosphate synthetase, highly similar to NP_302483.1|NC_002677 polyprenyl diphosphate synthase component from Mycobacterium leprae (330 aa). Also similar to others (generally hepta or hexaprenyl e.g. NP_471378.1|NC_003212 protein similar to heptaprenyl diphosphate synthase component II (menaquinone biosynthesis) from Listeria innocua (321 aa); NP_371994.1|NC_002758 heptaprenyl diphosphate syntase component II from Staphylococcus aureus subsp. aureus Mu50 (319 aa); P55785|HEP2_BACST heptaprenyl diphosphate synthase component from Bacillus subtilis (323 aa), FASTA scores: opt: 496, E(): 1.4e-24, (31.4% identity in 306 aa overlap); etc. Also highly similar to Mycobacterium tuberculosis proteins e.g. Rv0562|grcC1|NP_215076.1|MTCY25D10.41 probable polyprenyl-diphosphate synthase (335 aa); Rv3383, Rv3398c, Rv2173, etc. Seems to belong to the FPP/GGPP synthetases family. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O05572 No intermediary metabolism and respiration O05572 O05572 GO:0016740,GO:0008299 2.5.1.- O05572 NC_000962.3 Mycobrowser_v5 CDS 1109272 1110192 . + 0 Rv0993 galU May play a role in stationary phase survival [catalytic activity: UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose]. UTP--glucose-1-phosphate uridylyltransferase GalU (UDP-glucose pyrophosphorylase) (UDPGP) (alpha-D-glucosyl-1-phosphate uridylyltransferase) (uridine diphosphoglucose pyrophosphorylase) Rv0993, (MTCI237.07), len: 306 aa. GalU, UTP--glucose-1-phosphate uridylyltransferase, equivalent to AL035500|MLCL373_22 putative UTP-glucose-1-phosphate uridylyltransferase from Mycobacterium leprae (306 aa), FASTA score: (89.7% identity in 302 aa overlap). Also highly similar to others e.g. AB59678.1|AL132674 UTP-glucose-1-phosphate uridylyltransferase from Streptomyces coelicolor (303 aa); NP_244519.1|NC_002570 UTP-glucose-1-phosphate uridylyltransferase from Bacillus halodurans (297 aa); P25520|GALU_ECOLI|B1236|Z2012|ECS17 UTP--glucose-1-phosphate uridylyltransferase from Escherichia coli strains K12 and O157:H7 (301 aa), FASTA scores: opt: 624, E(): 2.4e-33, (38.8% identity in 299 aa overlap); etc. Belongs to the prokaryotic UDPGP family. O05576 No intermediary metabolism and respiration O05576 O05576 GO:0009058,GO:0003983 2.7.7.9 O05576 NC_000962.3 Mycobrowser_v5 CDS 1125444 1127003 . - 0 Rv1007c metS It is probably essential for cell survival, being required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [catalytic activity: ATP + L-methionine + tRNA(met) = AMP + diphosphate + L-methionyl-tRNA(met)] Methionyl-tRNA synthetase MetS (MetRS) (methionine--tRNA ligase) Rv1007c, (MTCI237.24), len: 519 aa. metS (MetG), methionyl-tRNA synthetase, similar to many e.g. SYM_BACSU|P37465 methionyl-tRNA synthetase from Bacillus subtilus (664 aa), FASTA scores: opt: 1506, E(): 0, (44.9% identity in 492 aa overlap); similar to other Mycobacterium tuberculosis tRNA synthases e.g. Rv2448c, Rv1536, Rv0041. Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Belongs to class-I aminoacyl-tRNA synthetase family. Strong, to cysteinyl-tRNA synthetase. P9WFU5 No information pathways O05593 P9WFU5 GO:0005737,GO:0004825,GO:0006431,GO:0005524 6.1.1.10 P9WFU5 NC_000962.3 Mycobrowser_v5 CDS 1114748 1115749 . + 0 Rv0998 Rv0998 Unknown Conserved hypothetical protein Rv0998, (MTCI237.12), len: 333 aa. Conserved hypothetical protein, with cyclic nucleotide-binding domain in N-terminal part and GNAT (Gcn5-related N-acetyltransferase) domain in C-terminal part. See Vetting et al. 2005. Possibly cyclic nucleotide-dependent protein kinase, highly similar to NP_301261.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (353 aa); and AL035500|MLCL373.38|T45457 hypothetical protein from Mycobacterium leprae (143 aa), FASTA score: (61.5% identity in 143 aa overlap). Also similar to many hypothetical proteins and cyclic-NMP-dependent protein kinases (generally at C-terminus) e.g. N-terminus of SC9B10.09|T35878 hypothetical protein from Streptomyces coelicolor (1039 aa); P05987|KAPR_DICDI camp-dependent protein kinase regulatory chain from Dictyostelium discoideum (327 aa), FASTA scores: opt: 177, E(): 0.00036, (32.0% identity in 122 aa overlap); NP_104403.1|NC_002678 hypothetical protein (contains similarity to cAMP-dependent protein kinase regulatory subunit) from Mesorhizobium loti (151 aa); etc. Contains PS00889 Cyclic nucleotide-binding domain signature 2. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O05581 No conserved hypotheticals O05581 O05581 GO:0008152,GO:0008080 O05581 NC_000962.3 Mycobrowser_v5 CDS 1115767 1116525 . + 0 Rv0999 Rv0999 Unknown Unknown protein Rv0999, (MTCI237.13), len: 252 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). O05582 No conserved hypotheticals O05582 O05582 NC_000962.3 Mycobrowser_v5 CDS 1131625 1133259 . + 0 Rv1013 pks16 Potentially involved in some intermediate steps for the synthesis of a polyketide molecule which may be involved in secondary metabolism. Putative polyketide synthase Pks16 Rv1013, (MTCI237.30-MTCY10G2.36c), len: 544 aa. Putative pks16, polyketide synthase, similar to many e.g. N-terminus of Q50857|U24657 saframycin MX1 synthetase B (1770 aa), FASTA scores: opt: 526, E(): 1.4e-25, (29.3% identity in 542 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family. O05598 No lipid metabolism O05598 O05598 GO:0008152,GO:0003824 O05598 NC_000962.3 Mycobrowser_v5 CDS 1134785 1135465 . - 0 Rv1016c lpqT Unknown Probable conserved lipoprotein LpqT Rv1016c, (MTCY10G2.33), len: 226 aa. Probable lpqT, conserved lipoprotein. Similar to several Mycobacterium tuberculosis hypothetical proteins e.g. Rv0040c|Y0H3_MYCTU|P71697 Proline rich 28 kDA antigen (310 aa), FASTA scores: opt: 329, E(): 2e-17, (32.3% identity in 229 aa overlap); Rv0583c. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WK59 No cell wall and cell processes P96384 P9WK59 GO:0005886 P9WK59 NC_000962.3 Mycobrowser_v5 CDS 1146561 1147028 . + 0 Rv1025 Rv1025 Function unknown Conserved protein Rv1025, (MTCY10G2.24c), len: 155 aa. Conserved protein, similar to hypothetical protein AE001768|AE001768_4 Thermotoga maritima (170 aa) FASTA scores: opt: 254, E(): 9.5e-10, (35.7% identity in 143 aa overlap). P96375 No conserved hypotheticals P96375 P96375 NC_000962.3 Mycobrowser_v5 CDS 1147019 1147978 . + 0 Rv1026 Rv1026 Unknown. Could be involved in an adaptive process that allows bacteria to respond to amino acid starvation. Conserved protein Rv1026, (MTCY10G2.23c), len: 319 aa. Conserved protein. Similar to GPPA_ECOLI|P25552 guanosine-5'-triphosphate,3'-diphosphate pyrophoshatase from Escherichia coli (494 aa), FASTA scores: opt: 281, E(): 3.2e-11, (30.6% identity in 291 aa overlap). Equivalent to AL023514|MLCB4.02 hypothetical protein from Mycobacterium leprae (317 aa) (77.9% identity in 321 aa overlap). P96374 No virulence, detoxification, adaptation P96374 P96374 GO:0016787 P96374 NC_000962.3 Mycobrowser_v5 CDS 1152012 1153727 . + 0 Rv1029 kdpA One of the components of the high-affinity ATP-driven potassium transport (or KDP) system, which catalyzes the hydrolysis of ATP coupled with the exchange of hydrogen and potassium ions [catalytic activity: ATP + H(2)O + K(+)(out) = ADP + phosphate + K(+)(in)]. Probable potassium-transporting ATPase a chain KdpA (potassium-translocating ATPase a chain) (ATP phosphohydrolase [potassium-transporting] a chain) (potassium binding and translocating subunit A) Rv1029, (MTCY10G2.20c), len: 571 aa. Probable kdpA, potassium-transporting ATPase a chain (transmembrane protein), similar to others e.g. ATKA_ECOLI|P03959|KDPA|B0698 potassium-transporting ATPase A chain from Escherichia coli strain K12 (557 aa), FASTA scores: opt: 1763, E(): 0, (50.4% identity in 569 aa overlap); etc. Belongs to the KdpA family. P9WKF3 No cell wall and cell processes P65209 P9WKF3 GO:0016021,GO:0005886,GO:0006813,GO:0008556,GO:0005524 3.6.3.12 P9WKF3 NC_000962.3 Mycobrowser_v5 CDS 1153724 1155853 . + 0 Rv1030 kdpB One of the components of the high-affinity ATP-driven potassium transport (or KDP) system, which catalyzes the hydrolysis of ATP coupled with the exchange of hydrogen and potassium ions [catalytic activity:ATP + H(2)O + K(+)(out) = ADP + phosphate + K(+)(in)]. Probable potassium-transporting P-type ATPase B chain KdpB (potassium-translocating ATPase B chain) (ATP phosphohydrolase [potassium-transporting] B chain) (potassium binding and translocating subunit B) Rv1030, (MTCY10G2.19c), len: 709 aa. Probable kdpB, potassium-transporting P-type ATPase B chain (transmembrane protein), similar to others e.g. ATKB_ECOLI|P03960 potassium-transporting ATPase B chain from Escherichia coli strain K12 (682 aa), FASTA scores: opt: 1481, E(): 0, (63.4% identity in 686 aa overlap); etc. Very similar to AL078610|SCH35.47 H+/K+-exchanging ATPase chain B from Streptomyces coelicolor (707 aa), FASTA scores: opt: 2731, E(): 0, (71.6% identity in 676 aa overlap). Contains PS00154 E1-E2 ATPases phosphorylation site. P9WPU3 No cell wall and cell processes P63681 P9WPU3 GO:0006754,GO:0016021,GO:0000287,GO:0005886,GO:0006813,GO:0008556,GO:0005524 3.6.3.12 P9WPU3 NC_000962.3 Mycobrowser_v5 CDS 1157963 1158736 . - 0 Rv1033c trcR Sensor part of the two component regulatory system TRCS/TRCR. Involved in transcriptional autoactivation: TRCR activates its own expression by interacting with the at-rich sequence of the TRCR promoter. Two component transcriptional regulator TrcR Rv1033c, (MTCY10G2.16), len: 257 aa. TrcR, two-component regulatory protein (see citations below), similar to Q50825 two component response regulator from Mycobacterium tuberculosis (234 aa), FASTA scores: opt: 628, E(): 0, (46.0% identity in 226 aa overlap). Note that in vitro autophosphorylation of TrcS requires the presence of Mn2+or Ca2+as a divalent cation cofactor and subsequent transphosphorylation of TrcR is evident in the presence of TrcS-phosphate and Ca2+. L7N689 No regulatory proteins Q50806 L7N689 GO:0006355,GO:0000156,GO:0000160,GO:0003677 L7N689 NC_000962.3 Mycobrowser_v5 CDS 1176011 1176286 . - 0 Rv1053c Rv1053c Unknown Hypothetical protein Rv1053c, (MTV017.06c), len: 91 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53401 No conserved hypotheticals O53401 O53401 NC_000962.3 Mycobrowser_v5 CDS 1176928 1177242 . + 0 Rv1054 Rv1054 Use for sequence integration. Integrase is necessary for integration of a phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome. Probable integrase (fragment) Rv1054, (MTV017.07), len: 104 aa. Probable integrase (fragment), similar to Rv2309c|MTCY3G12_25|Z79702 hypothetical protein (shows similarity to integrases) from Mycobacterium tuberculosis (151 aa), FASTA scores: opt: 273, E(): 8.8e-13, (64.7% identity in 68 aa overlap); and to L39071|MSGINT_1 integrase from Mycobacterium paratuberculosis (191 aa), FASTA scores: opt: 105, E(): 0.9, (31.8% identity in 85 aaoverlap). This ORF continues in another frame as Rv1055|MTV017.08 but no error can be found to account for frameshift. Length extended since first submission (+36 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53402 No insertion seqs and phages O53402 NC_000962.3 Mycobrowser_v5 CDS 1783620 1783892 . - 0 Rv1580c Rv1580c Unknown Probable PhiRv1 phage protein Rv1580c, (MTCY336.24), len: 90 aa. Probable phiRv1 phage protein (see citation below). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06610 No insertion seqs and phages O06610 O06610 NC_000962.3 Mycobrowser_v5 CDS 1185741 1186823 . - 0 Rv1063c Rv1063c Function unknown Conserved hypothetical protein Rv1063c, (MTV017.16c), len: 360 aa. Conserved hypothetical protein, similar to P37053|YCHK_ECOLI hypothetical protein from Escherichia coli (314 aa), FASTA scores: opt: 487, E(): 7.2e-23, (32.7% identity in 321 aa overlap). Also partially similar to Rv3239c|MTCY20B11.14c. Belongs to the UPF0028 (SWS) family. P9WIY9 No conserved hypotheticals P67098 P9WIY9 GO:0004622,GO:0046470,GO:0005789 NC_000962.3 Mycobrowser_v5 CDS 1188421 1190424 . - 0 Rv1067c PE_PGRS19 Function unknown PE-PGRS family protein PE_PGRS19 Rv1067c, (MTV017.20c), len: 667 aa. PE_PGRS19, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002). Similar to Rv3388|MTV004.46 M. tuberculosis (731 aa), FASTA scores: opt: 2227, E(): 0, (55.6% identity in 710 aa overlap). Contains PS00583 pfkB family of carbohydrate kinases signature 1, probably fortuitous. Predicted to be an outer membrane protein (See Song et al., 2008). Q79FT3 No PE/PPE Q79FT3 Q79FT3 Q79FT3 NC_000962.3 Mycobrowser_v5 CDS 1195055 1196092 . - 0 Rv1071c echA9 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Possible enoyl-CoA hydratase EchA9 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv1071c, (MTV017.24c), len: 345 aa. Possible echA9, enoyl-CoA hydratase, equivalent to Y13803|B1306.06c putative enoyl-CoA hydratase/isomerase from Mycobacterium leprae (345 aa), FASTA scores: opt: 1799, E(): 0, (77.7% identity in 345 aa overlap). Also similar to many eukaryotic and prokaryotic enoyl-CoA hydratases e.g. NP_437984.1|NC_003078 putative enoyl-CoA hydratase protein from Sinorhizobium meliloti (356 aa); NP_420165.1|NC_002696 enoyl-CoA hydratase/isomerase family protein from Caulobacter crescentus (350 aa); Q19278 protein similar to enoyl-CoA hydratases from Caenorhabditis elegans (386), FASTA scores: opt: 787, E(): 0, (38.5% identity in 348 aa overlap); etc. O53419 No lipid metabolism O53419 O53419 GO:0016853,GO:0008152,GO:0004300 4.2.1.17 O53419 NC_000962.3 Mycobrowser_v5 CDS 1210595 1211383 . + 0 Rv1086 Rv1086 Catalyzes the first committed step in the synthesis of decaprenyl diphosphate, a molecule which has a central role in the biosynthesis of most features of the mycobacterial cell wall. Adds one isoprene unit to omega,E-geranyl diphosphate. The product, omega,E, Z-farnesyl diphosphate, is the putative substrate of Rv2361c product [catalytic activity: geranyl diphosphate + isopentenyl diphosphate = diphosphate + trans,trans-farnesyl diphosphate]. Short (C15) chain Z-isoprenyl diphosphate synthase (Z-FPP synthase) (Z-farnesyl diphosphate synthase) (Z-FPP synthetase) (Z-farnesyl diphosphate synthetase) (geranyltranstransferase) (farnesyl pyrophosphate synthetase) Rv1086, (MTV017.39), len: 262 aa. Short (C15) chain Z-isoprenyl diphosphate synthase (see citations below), equivalent to NP_302598.1|NC_002677 possible undecaprenyl pyrophosphate synthetase from Mycobacterium leprae (262 aa), similar to many hypothetical proteins and several potential members of the upp synthase family e.g. NP_296167.1|NC_001263 undecaprenyl diphosphate synthase from Deinococcus radiodurans (339 aa); P20182|YT14_STRFR Hypothetical protein from Streptomyces fradiae (259 aa), FASTA scores: opt: 840, E(): 0, (51.0% identity in 259 aa overlap); and P38118|YARF_CORGL Hypothetical protein from Corynebacterium glutamicicum (234 aa), FASTA scores: opt: 729, E(): 0, (56.0% identity in 209 aa overlap); etc. Also similar to Rv2361c|MTCY27.19 (296 aa) (35.6% identity in 233 aa overlap). Contains PS01066 Uncharacterized protein family UPF0015 signature. Seems to belong to the UPP synthetase family. P9WFF5 No cell wall and cell processes 2VFW,2VG0,2VG1 O53434 P9WFF5 GO:0007047,GO:0009252,GO:0008360,GO:0033850 2.5.1.68 P9WFF5 NC_000962.3 Mycobrowser_v5 CDS 1197231 1198082 . + 0 Rv1073 Rv1073 Function unknown Conserved hypothetical protein Rv1073, (MTV017.26), len: 283 aa. Conserved hypothetical protein, similar to several hypothetical mycobacterial proteins e.g. Rv1482c|Z79701|MTCY277.03 Mycobacterium tuberculosis (339 aa), FASTA scores: opt: 810, E(): 0, (47.4% identity in 272 aa overlap); Rv3555c|Z92774|MTCY6G11_2 Mycobacterium tuberculosis (289 aa), FASTA scores: opt: 704, E(): 0, (44.4% identity in 259 aa overlap); and Rv3517, etc., and GIR10|AF002133_10 Mycobacterium avium strain GIR10 (346 aa), FASTA scores: opt: 802, E(): 0, (48.1% identity in 270 aa overlap). O53421 No conserved hypotheticals O53421 O53421 NC_000962.3 Mycobrowser_v5 CDS 1198156 1199373 . - 0 Rv1074c fadA3 Function unknown, but supposedly involved in lipid degradation (beta oxidation). Probable beta-ketoacyl CoA thiolase FadA3 Rv1074c, (MTV017.27c), len: 405 aa. Probable fadA3, beta-ketoacyl CoA thiolase, highly similar to many involved in beta-oxidation e.g. CAB89028.1|AL353870 beta-ketoadipyl-CoA thiolase from Streptomyces coelicolor (395 aa); P77525|PAAJ_ECOLI probable beta-ketoadipyl CoA thiolase from Escherichia coli (401 aa), FASTA scores: opt: 1034, E(): 5.4e-56, (43.5% identity in 416 aa overlap) and X97452 acetyl-CoA acetyltransferase (thiolase) from Escherichia coli (401 aa), FASTA scores: opt: 1043, E(): 0, (43.4% identity in 415 aa overlap); Q43935|CATF_ACICA beta-ketoadipyl CoA thiolase from Acinetobacter calcoaceticus (401 aa), FASTA scores: opt: 992, E(): 0, (41.5% identity in 415 aa overlap); etc. Contains PS00737 Thiolases signature 2, and PS00445 FGGY family of carbohydrate kinases signature 2, although this is probably fortuitous. Belongs to the thiolase family. O53422 No lipid metabolism O53422 O53422 GO:0008152,GO:0008415 2.3.1.- O53422 NC_000962.3 Mycobrowser_v5 CDS 1201717 1203111 . + 0 Rv1077 cbs Thought to be involved in homocysteine transulfuration [catalytic activity: L-serine + L-homocysteine = cystathionine + H2O] Probable cystathionine beta-synthase Cbs (serine sulfhydrase) (beta-thionase) (hemoprotein H-450) Rv1077, (MTV017.30), len: 464 aa. Probable cbs (previously cysM2), cystathionine beta-synthase, similar throughout its length to many eukaryotic cystathionine beta-synthases e.g. P32232|CBS_RAT cystathionine beta-synthase (560 aa), FASTA scores: opt: 951, E(): 0, (40.2% identity in 450 aa overlap); also similar in N-terminal domain (aa 1 - 330) to Rv2334|MTCY98.03 CysK Mycobacterium tuberculosis (310 aa), FASTA scores: opt: 855, E(): 0, (46.8% identity in 314 overlap); and other cysteine synthase proteins e.g. Rv1336, Rv0848, etc. Contains PS00217 Sugar transport proteins signature 2 probably spurious. Belongs to the cysteine synthase/cystathionine beta-synthase family. P9WP51 No intermediary metabolism and respiration Q7D8W0 P9WP51 GO:0019343,GO:0005737,GO:0030170,GO:0004122 4.2.1.22 P9WP51 NC_000962.3 Mycobrowser_v5 CDS 1206520 1207386 . + 0 Rv1082 mca Mycothiol-dependent detoxification enzyme, involved in mycothiol biosynthesis. Mycothiol conjugate amidase Mca (mycothiol S-conjugate amidase) Rv1082, (MTV017.35), len: 288 aa. Mca, mycothiol conjugate amidase (see citation below), equivalent to NP_302547.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (290 aa), FASTA scores: opt: 1737, E(): 0, (86.4% identity in 287 aa overlap); and similar to Q54358|X79146 lmbE protein from Streptomyces lincolnensis (270 aa). Also similar to Rv1170|MTV005.06|MSHB GlcNAc-Ins deacetylase from Mycobacterium tuberculosis (303 aa), FASTA scores: opt: 411, E(): 9.4e-20, (35.8% identity in 299 aa overlap). P9WJN1 No virulence, detoxification, adaptation O53430 P9WJN1 P9WJN1 NC_000962.3 Mycobrowser_v5 CDS 1221959 1222786 . + 0 Rv1094 desA2 Thought to catalyze the principal conversion of saturated fatty acids to unsaturated fatty acids. Thought to convert stearoyl-ACP to oleoyl-ACP by introduction of a cis double bond between carbons delta-9 and delta-10 of the acyl chain [catalytic activity: stearoyl-[acyl-carrier protein] + AH2 + O2 = oleoyl-[acyl-carrier protein] + a + 2 H2O]. Possible acyl-[acyl-carrier protein] desaturase DesA2 (acyl-[ACP] desaturase) (stearoyl-ACP desaturase) Rv1094, (MTV017.47), len: 275 aa. Possible desA2, acyl-[acyl-carrier protein] desaturase (stearoyl-ACP desaturase), equivalent to AL049491|MLCB1222_15 from Mycobacterium leprae (275 aa), FASTA score: (78.1% identity in 274 aa overlap). Also weakly similar to plant stearoyl-acyl carrier protein desaturases, and very similar to U49839|MTV043.16C|Rv0824c enzyme desA1 from Mycobacterium tuberculosis (338 aa), FASTA scores: opt: 525, E(): 8.5e-30, (32.2% identity in 270 aa overlap); and to U15182|MLU15182_32 acyl-carrier protein desaturase precursor from Mycobacterium leprae (338 aa), FASTA scores: opt: 506, E(): 1.9e-28, (34.1% identity in 261 aa overlap). P9WNZ5 No lipid metabolism 1ZA0 O53442 P9WNZ5 GO:0006633,GO:0055114,GO:0046914,GO:0045300 1.14.19.- P9WNZ5 NC_000962.3 Mycobrowser_v5 CDS 1225263 1226144 . - 0 Rv1097c Rv1097c Unknown Probable membrane glycine and proline rich protein Rv1097c, (MTV017.50c), len: 293 aa. Probable membrane Gly-, Pro-rich protein, similar to Mycobacterium tuberculosis Rv2507|MTCY07A7. 13|Z95556 (273 aa), FASTA scores: opt: 219, E(): 0.023, (30.5% identity in 266 aa overlap); and Rv2507. Contains potential membrane spanning region (aa ~68-92). O53445 No cell wall and cell processes O53445 NC_000962.3 Mycobrowser_v5 CDS 1247127 1248077 . + 0 Rv1124 ephC Thought to be involved in detoxification reactions following oxidative damage to lipids [catalytic activity: an epoxide + H(2)O = a glycol]. Probable epoxide hydrolase EphC (epoxide hydratase) Rv1124, (MTCY22G8.13), len: 316 aa. Probable ephC, epoxide hydrolase (see citation below), similar to Q42566 epoxide hydrolase from Arabidopsis thaliana (321 aa), FASTA scores: opt: 298, E(): 8.2e-13, (27.6% identity in 333 aa overlap). Similar to other M. tuberculosis epoxide hydrolases and non-heme bromoperoxidases e.g. Rv1938, Rv3617, Rv3670, Rv3473c, etc. O06576 No virulence, detoxification, adaptation O06576 O06576 GO:0016787 3.3.2.3 O06576 NC_000962.3 Mycobrowser_v5 CDS 1226141 1227565 . - 0 Rv1098c fum Involved in the tricarboxylic acid cycle. Catalyzes the reversible hydration of fumarate to L-malate [catalytic activity: (S)-malate = fumarate + H2O] Probable fumarase Fum (fumarate hydratase) Rv1098c, (MTV017.51c), len: 474 aa. Probable fum, fumarase. Equivalent to AL049491|MLCB1222_11 Mycobacterium leprae (474 aa) (89.5 % identity in 467 aa overlap). Similar to many e.g. P14408|FUMH_RAT fumarate hydratase, mitochondrial precursor from Rattus norvegicus (507 aa), FASTA scores: opt: 1427, E(): 0, (52.3% identity in 461 aa overlap); and P05042|FUMC_ECOLI Fumarate hydratase class II from Escherichia coli (467 aa), FASTA scores: opt: 1355, E(): 0, (50.2% identity in 444 aa overlap). Contains PS00163 Fumarate lyases signature. P9WN93 No intermediary metabolism and respiration 3NO9 O53446 P9WN93 GO:0004333,GO:0006099,GO:0005737 4.2.1.2 P9WN93 NC_000962.3 Mycobrowser_v5 CDS 1232844 1233956 . - 0 Rv1106c Rv1106c Oxidizes 3-beta-hydroxysterols using NAD+ as cofactor 3-beta-hydroxysteroid dehydrogenase Rv1106c, (MTV017.59c), len: 370 aa. 3-beta-hydroxysteroid dehydrogenase (see Yang et al., 2007). Equivalent to AL049491|MLCB1222_7 Mycobacterium leprae (376 aa) (75.5% identity in 375 aa overlap). Highly similar to Q03704 NAD(P)-dependent cholesterol dehydrogenase from Nocardia sp. (364 aa), FASTA scores: opt: 1789, E(): 0, (74.5% identity in 361 aa overlap). Also similar to U32426|MCU32426_1 3-beta-hydroxy-Delta5-steroid dehydrogenase from Molluscum contagiosum virus (354 aa), FASTA scores: opt: 432, E(): 1.7e-22, (34.6% identity in 347 aa overlap). Also similar to series of Mycobacterium tuberculosis hypothetical proteins described as sugar epimerases or dehydratases e.g. Rv3634c, Rv3784, Rv3464, etc. The transcription of this CDS seems to be activated specifically in host granulomas (see Ramakrishnan et al., 2000). P9WQP7 No intermediary metabolism and respiration O53454 P9WQP7 GO:0016853,GO:0055114,GO:0006694,GO:0003854 1.1.1.- P9WQP7 NC_000962.3 Mycobrowser_v5 CDS 1357293 1357625 . - 0 Rv1214c PE14 Function unknown PE family protein PE14 Rv1214c, (MTCI364.26c), len: 110 aa. PE14, Member of Mycobacterium tuberculosis PE family (see citation below), appears to be frameshifted but sequence appears to be correct. The 5'-end is atypical as first 9 aa appear to be missing. L7N6A7 No PE/PPE Q7D8L5 L7N6A7 L7N6A7 NC_000962.3 Mycobrowser_v5 CDS 1236185 1237192 . + 0 Rv1110 lytB2 Unknown. In other organisms, LYTB product is involved in penicillin tolerance and control of the stringent response. Probable LYTB-related protein LytB2 Rv1110, (MTV017.63), len: 335 aa. Probable lytB2, LytB-related protein, equivalent to AL049491|MLCB1222_3 from Mycobacterium leprae (335 aa), FASTA score: (82.9% identity in 333 aa overlap). Also similar to LytB proteins from many bacteria (appears to have N-terminal extension) e.g. P22565|LYTB_ECOLI|B0029|Z0034|ECS0032 LYTB protein from Escherichia coli strains K12 and O157:H7 (316 aa), FASTA scores: opt: 1041, E():0, (52.4% identity in 309 aa overlap); etc. Also very similar to another LytB-related protein from Mycobacterium tuberculosis: LytB1|Rv3382c|MTV004.40c (329 aa), FASTA scores: opt: 975, E(): 0, (51.3% identity in 312 aa overlap). Belongs to the LytB family. P9WKG1 No cell wall and cell processes P0A5I0 P9WKG1 GO:0051745,GO:0019288,GO:0046872,GO:0055114,GO:0016114,GO:0051538 1.17.1.2 P9WKG1 NC_000962.3 Mycobrowser_v5 CDS 1249932 1251404 . - 0 Rv1127c ppdK Catalyzes the reversible phosphorylation of pyruvate and phosphate [catalytic activity: ATP + pyruvate + phosphate = AMP + phosphoenolpyruvate + diphosphate] Probable pyruvate, phosphate dikinase PpdK Rv1127c, (MTCY22G8.16c), len: 490 aa. Probable ppdK, Pyruvate, phosphate dikinase. Equivalent (but shorter) to Z94723|MLCB33_16 ppdK from Mycobacterium leprae (601 aa) (71.8% identity in 478 aa overlap). Highly similar to N-terminus of PODK_CLOSY|P22983 pyruvate, phosphate dikinase from Clostridium symbiosum (873 aa), FASTA scores: opt: 786, E(): 0, (37.4% identity in 514 aa overlap). O06579 No intermediary metabolism and respiration O06579 O06579 GO:0016301,GO:0016310,GO:0050242,GO:0005524 2.7.9.1 O06579 NC_000962.3 Mycobrowser_v5 CDS 1256132 1257313 . + 0 Rv1131 prpC Involved in methyl citrate cycle [catalytic activity: propionyl-CoA + oxaloacetate + H2O = 2-methylcitrate + CoA] Probable methylcitrate synthase PrpC Rv1131, (MTCY22G8.20), len: 393 aa. Probable prpC, methylcitrate synthase (MCS) (previously known as gltA1) , highly similar to CISY_MYCSM|P26491 citrate synthase from Mycobacterium smegmatis (375 aa), FASTA scores: opt:1942, E(): 0, (80.0% identity in 375 aa overlap). Also similar to two other M. tuberculosis citrate synthases, Rv0896c|MTCY31.24|gltA2 (431 aa), FASTA score: (33.1% identity in 381 aa overlap) and Rv0889|MTCY31.17c|citA (373 aa), FASTA score: (31.8% identity in 371 aa overlap). Contains PS00480 Citrate synthase signature. Belongs to the citrate synthase family. I6Y9Q3 No intermediary metabolism and respiration 3HWK O08395 I6Y9Q3 GO:0044262,GO:0004108,GO:0005737,GO:0006099,GO:0008415 2.3.3.1 I6Y9Q3 NC_000962.3 Mycobrowser_v5 CDS 1259067 1261346 . - 0 Rv1133c metE Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation (pathway: terminal step in the de novo biosynthesis of methionine) [catalytic activity: 5-methyltetrahydropteroyltri-L-glutamate + L- homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine.] Probable 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase MetE (methionine synthase, vitamin-B12 independent isozyme) Rv1133c, (MTC22G8.22), len: 759 aa (start site chosen by homology). Probable metE, 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase, highly similar to others e.g. METE_ECOLI|P25665 Escherichia coli (752 aa), FASTA scores: opt: 2251, E(): 0, (48.1% identity in 756 aa overlap). Equivalent to Z94723|MLCB33_14 metE from M. leprae (760 aa) (85.3% identity in 755 aa overlap). Belongs to the vitamin-B12 independent methionine synthase family. P9WK07 No intermediary metabolism and respiration P65340 P9WK07 GO:0009086,GO:0008270,GO:0003871 2.1.1.14 P9WK07 NC_000962.3 Mycobrowser_v5 CDS 1269152 1269958 . - 0 Rv1142c echA10 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Probable enoyl-CoA hydratase EchA10 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv1142c, (MTCI65.09c), len: 268 aa. Probable echA10, enoyl-CoA hydratase, similar to others e.g. CAA66096.1|X97452 enoyl-CoA isomerase from Escherichia coli (262 aa), FASTA scores: opt: 525, E(): 1.3e-26, (35.1% identity in 251 aa overlap); NP_420658.1|NC_002696 enoyl-CoA hydratase/isomerase family protein from Caulobacter crescentus (267 aa); NP_438092.1|NC_003078 putative enoyl-CoA hydratase protein from Sinorhizobium meliloti (263 aa); etc. Also similarity with naphthoate synthases. Also highly similar to upstream ORF Rv1141c|MTCI65.08c|echA11 probable enoyl-CoA hydratase from Mycobacterium tuberculosis (268 aa), FASTA score: opt: 1225, E(): 0, (72.3% identity in 267 aa overlap). O06542 No lipid metabolism O06542 O06542 GO:0016853,GO:0008152,GO:0004300 4.2.1.17 O06542 NC_000962.3 Mycobrowser_v5 CDS 1273355 1274767 . + 0 Rv1146 mmpL13b Unknown. Thought to be involved in fatty acid transport. Probable conserved transmembrane transport protein MmpL13b Rv1146, (MTCI65.13), len: 470 aa. Probable mmpL13b, conserved transmembrane transport protein (see citation below), member of RND superfamily, showing some similarity to putative Mycobacterial and Streptomyces membrane proteins e.g. Q53902|C40046 antibiotic transport-associated protein from Streptomyces coelicolor (711 aa), FASTA scores: opt: 193, E(): 2.1e-05, (28.9% identity in 394 aa overlap); etc. Could be in frame with previous ORF mmpL13A|Rv1145, but no sequence error apparent to account for this; sequence is identical in M. tuberculosis strain CDC1551, and Mycobacterium bovis strain AF2122/97. Belongs to the MmpL family. O06546 No cell wall and cell processes O06546 O06546 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1937313 1938149 . + 0 Rv1709 scpA Involved in chromosome structure and partitioning Possible segregation and condensation protein ScpA Rv1709, (MTCI125.31), len: 278 aa. Possible scpA, segregation and condensation protein, similar to e.g. P35154|YPUG_BACSU from Bacillus subtilis (251 aa), FASTA scores: opt: 271, E(): 8.2e-10, (27.0% identity in 248 aa overlap); Q9S230|SCI51.10C|AL109848 from Streptomyces coelicolor (264 aa), FASTA scores: opt: 855, E(): 0, (56.8% identity in 257 aa overlap). Equivalent to Q49888|MLC1351.05C|Z95117 from Mycobacterium leprae (268 aa), FASTA scores: (78.9% identity in 251 aa overlap). O33208 No cell wall and cell processes O33208 O33208 GO:0051301,GO:0007059,GO:0005737,GO:0007049 O33208 NC_000962.3 Mycobrowser_v5 CDS 1284179 1284862 . - 0 Rv1158c Rv1158c Function unknown Conserved hypothetical ala-, pro-rich protein Rv1158c, (MTCI65.25c), len: 227 aa. Conserved hypothetical Ala-, Pro-rich protein, similar to other proline rich proteins and extensins e.g. MMSAP62|g633250 house mouse (485 aa), FASTA scores: opt: 367, E(): 1.2e-08, (36.3% identity in 212 aa overlap). Has hydrophobic stretch at N-terminus suggestive of secretion signal. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O06556 No conserved hypotheticals O06556 O06556 NC_000962.3 Mycobrowser_v5 CDS 1284992 1286287 . + 0 Rv1159 pimE Polyprenol-phosphate-mannose dependent mannosyltransferase involved in phosphatidylinositol mannoside synthesis Mannosyltransferase PimE Rv1159, (MTCI65.26), len: 431 aa. PimE, mannosyltransferase (see Morita et al., 2006) Conserved transmembrane protein, similar to others in Mycobacterium tuberculosis e.g. Rv2181|MTCY21D4.13 (560 aa), FASTA scores: opt: 172; E(): 0.00035, (25.0% identity in 332 aa overlap). Belongs to the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). P9WN01 No cell wall and cell processes O06557 P9WN01 GO:0009405,GO:0008654,GO:0005886,GO:0016740,GO:0016021 2.4.1.- P9WN01 NC_000962.3 Mycobrowser_v5 CDS 1291065 1292741 . + 0 Rv1162 narH Nitrate reduction [catalytic activity: nitrite + acceptor = nitrate + reduced acceptor]. Probable respiratory nitrate reductase (beta chain) NarH Rv1162, (MTCI65.29), len: 558 aa. Probable narH, respiratory nitrate reductase beta chain. Similar to others e.g. NARH_BACSU|P42176 nitrate reductase beta chain from Bacillus subtilis (487 aa), FASTA scores: opt: 2049, E(): 0, (56.8% identity in 488 aa overlap); etc. Contains PS00190 cytochrome c family heme-binding site signature. O06560 No intermediary metabolism and respiration O06560 GO:0008940,GO:0009325,GO:0055114,GO:0042126 1.7.99.4 O06560 NC_000962.3 Mycobrowser_v5 CDS 1293406 1294146 . + 0 Rv1164 narI Nitrate reduction [catalytic activity: nitrite + acceptor = nitrate + reduced acceptor]. Probable respiratory nitrate reductase (gamma chain) NarI Rv1164, (MTCI65.31), len: 246 aa. Probable narI, respiratory nitrate reductase gamma chain. Similar to others e.g. NARI_BACSU|P42177 nitrate reductase gamma chain from Bacillus subtilis (223 aa), FASTA scores: opt: 652, E(): 0; (41.6% identity in 221 aa overlap); etc. Highly similar to C-terminal part of Rv1736c|MTCY04C12.21c|NARX probable nitrate reductase (gamma chain) from Mycobacterium tuberculosis (68.6% identity in 239 aa overlap). O06562 No intermediary metabolism and respiration O06562 O06562 GO:0009325,GO:0055114,GO:0008940 1.7.99.4 O06562 NC_000962.3 Mycobrowser_v5 CDS 1370920 1371477 . + 0 Rv1228 lpqX Unknown Probable lipoprotein LpqX Rv1228, (MTCI61.11), len: 185 aa. Probable lipoprotein LpqX. Contains possible signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O33224 No cell wall and cell processes O33224 O33224 NC_000962.3 Mycobrowser_v5 CDS 1294168 1296054 . + 0 Rv1165 typA Unknown; probably interacts with the ribosomes in a GTP dependent manner Possible GTP-binding translation elongation factor TypA (tyrosine phosphorylated protein A) (GTP-binding protein) Rv1165, (MTV005.01-MTCI65.32), len: 628 aa. Possible typA (alternate gene name: bipA), GTP-binding translation elongation factor, similar to several e.g. P32132|TYPA_ECOLI|BIPA|B387 Escherichia coli (591 aa); YIHK_SYNY3|P72749 gtp-binding protein TYPA/BIPA homolog from synechocystis sp. (597 aa), FASTA scores: E(): 0, (46.9% identity in 610 aa overlap); and to elongation factor EF-G from many organims e.g. EFG_MICLU|P09952 micrococcus luteus (701 aa), FASTA scores: E(): 3e-24, (29.8% identity in 500 aa overlap). Belongs to the GTP-binding elongation factor family, TYPA subfamily. O06563 No information pathways O06563 O06563 GO:0003924,GO:0005622,GO:0003746,GO:0005525 O06563 NC_000962.3 Mycobrowser_v5 CDS 1300304 1301215 . + 0 Rv1170 mshB Involved in mycothiol biosynthesis. 1-D-myo-inosityl-2-acetamido-2-deoxy-alpha-D-glucopyranoside (GlcNAc-Ins) is converted to 1-D-myo-inosityl-2-amino-2-deoxy-alpha-D-glucopyranoside (GlcN-Ins) by this enzyme. Seems to possess weak mycothiol conjugate amidase activity but SHOWS substantial deacetylation activity with 1-D-myo-inosityl-2-acetamido-2-deoxy-alpha-D-glucopyranoside (GlcNAc-Ins), a hypothetical mycothiol biosynthetic precursor. GlcNAc-Ins is an intermediate in MSH biosynthesis. N-acetyl-1-D-myo-inosityl-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase MshB (GlcNAc-Ins deacetylase) Rv1170, (MTV005.06), len: 303 aa. MshB, N-Acetyl-1-D-myo-Inosityl-2-Amino-2-Deoxy-alpha-D-Glucopyranoside Deacetylase (GlcNAc-Ins deacetylase) (see citation below), similar to Q54358|X79146 lmbE gene from Streptomyces lincolnensis (270 aa), FASTA scores: opt: 308, E(): 1.2e-15, (32.0% identity in 278 aa overlap). Also similar to Rv1082|MCA Mycothiol conjugate amidase from Mycobacterium tuberculosis (288 aa). P9WJN3 No intermediary metabolism and respiration 1Q74,1Q7T O50426 P9WJN3 P9WJN3 NC_000962.3 Mycobrowser_v5 CDS 1302931 1305501 . + 0 Rv1173 fbiC Essential for coenzyme F420 production: participates in a portion of the F420 biosynthetic pathway between pyrimidinedione and FO (biosynthesis intermediate), before the deazaflavin ring is formed. Probable F420 biosynthesis protein FbiC Rv1173, (MTV005.09), len: 856 aa. Probable fbiC, F420 biosynthesis protein, equivalent to AAL91922|FBIC F420 biosynthesis protein fbiC from Mycobacterium bovis BCG (856 aa) (see citation below). The N-terminus (aa 80-420) is similar to Y446_METJA|Q57888 hypothetical protein mj0446 from methanococcus jannaschii (361 aa), FASTA scores: opt: 801, E(): 0, (41.2% identity in 337 aa overlap); and the C-terminus region (aa 530-856) is similar to e.g. YE31_METJA|Q58826 hypothetical protein mj1431 from methanococcus jannaschii (359 aa), FASTA scores: opt: 1089, E(): 0, (48.7% identity in 337 aa overlap). P9WP77 No intermediary metabolism and respiration O50429 P9WP77 GO:0009108,GO:0046872,GO:0016765,GO:0051539 2.5.1.- P9WP77 NC_000962.3 Mycobrowser_v5 CDS 1394179 1395918 . + 0 Rv1250 Rv1250 Thought to be involved in transport of drug across the membrane (export): drug resistance by an export mechanism (conferes resistance to toxic compounds by removing them for the cells). Responsible for the translocation of the substrate across the membrane. Probable drug-transport integral membrane protein Rv1250, (MTV006.22), len: 579 aa. Probable drug-transport integral membrane protein, member of major facilitator superfamily (MFS), highly similar to several including P39886|TCMA_STRGA tetracenomycin C resistance protein from Streptomyces glaucescens (538 aa), FASTA scores: opt: 847, E(): 0, (32.9% identity in 517 aa overlap); etc. Also similar to MTCY20B11.14c|Rv3239C from Mycobacterium tuberculosis (1048 aa), FASTA scores: opt: 629, E(): 6.7e-13, (31.9% identity in 423 aa overlap). P9WG87 No cell wall and cell processes O50465 P9WG87 GO:0005886,GO:0055085,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1309364 1310452 . + 0 Rv1178 Rv1178 Function unknown; probably involved in cellular metabolism. Probable aminotransferase Rv1178, (MTV005.14), len: 362 aa. Probable aminotransferase, weak similarity to many aspartate aminotransferases e.g. Q55679|D64000 SLL0006 aspartate aminotransferase from Synechocystis sp. (394 aa), FASTA scores: opt: 218, E(): 1.3e-25, (32.5% identity in 379 aa overlap). Contains PS00105 Aminotransferases class-I pyridoxal-phosphate attachment site. Also similar to Mycobacterium tuberculosis aminotransferases Rv2294, Rv0075, etc. O50434 No intermediary metabolism and respiration O50434 O50434 GO:0009058,GO:0030170,GO:0008483,GO:0016847 2.6.1.- O50434 NC_000962.3 Mycobrowser_v5 CDS 1310480 1313299 . - 0 Rv1179c Rv1179c Unknown Unknown protein Rv1179c, MTV005.15c, len: 939 aa. Unknown protein. O50435 No conserved hypotheticals O50435 O50435 GO:0003677,GO:0016787,GO:0005524 NC_000962.3 Mycobrowser_v5 CDS 1321520 1324528 . + 0 Rv1183 mmpL10 Unknown. Thought to be involved in fatty acid transport. Probable conserved transmembrane transport protein MmpL10 Rv1183, (MTV005.19), len: 1002 aa. Probable mmpL10, conserved transmembrane transport protein (see Tekaia et al., 1999), member of RND superfamily, similar to many Mycobacterial hypothetical membrane proteins e.g. Q49619|U00010 from Mycobacterium leprae (1008 aa), FASTA scores: opt: 4545, E(): 0, (70.6% identity in 978 aa overlap); etc. Belongs to the MmpL family. P9WJU1 No cell wall and cell processes P65372 P9WJU1 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1329390 1331021 . + 0 Rv1187 rocA Involved in the arginase pathway [catalytic activity: 1-pyrroline-5-carboxylate + NAD(+) + H(2)O = L-glutamate + NADH] Probable pyrroline-5-carboxylate dehydrogenase RocA Rv1187, (MTV005.23), len: 543 aa. Probable rocA, pyrroline-5-carboxylate dehydrogenase, similar to many e.g. PUT2_HUMAN|P30038 human delta-1-pyrroline-5-carboxylate dehydrogenase (563 aa), FASTA scores: opt: 1596, E():0, (46.0% identity in 531 aa overlap). Also similar to other Mycobacterium tuberculosis hypothetical dehydrogenases e.g. Rv0768, Rv2858c, etc. Contains PS00687 Aldehyde dehydrogenases glutamic acid active site and PS00070 Aldehyde dehydrogenases cysteine active site. O50443 No intermediary metabolism and respiration O50443 O50443 GO:0005759,GO:0055114,GO:0006561,GO:0003842 1.5.1.12 O50443 NC_000962.3 Mycobrowser_v5 CDS 1335794 1337215 . + 0 Rv1193 fadD36 Function unknown, but supposedly involved in lipid degradation. Probable fatty-acid-CoA ligase FadD36 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv1193, (MTCI364.05), len: 473 aa. Probable fadD36, fatty-acid-CoA synthetase, highly similar to Q50017|U15181 4-coumarate-CoA ligase from Mycobacterium leprae (476 aa), FASTA scores: opt: 2594, E(): 0, (81.3% identity in 476 aa overlap). Also highly similar to others e.g. CAB86109.1|AL163003 putative fatty acid synthase from Streptomyces coelicolor (485 aa); LCFA_ECOLI|P29212 long-chain-fatty-acid--CoA ligase from Escherichia coli (561 aa), FASTA scores: opt: 605, E(): 8.4e-30, (33.0% identity in 364 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family. O05295 No lipid metabolism O05295 O05295 GO:0008152,GO:0016874 6.2.1.- O05295 NC_000962.3 Mycobrowser_v5 CDS 1341358 1342605 . - 0 Rv1199c Rv1199c Possibly required for the transposition of the insertion element IS1081. Possible transposase Rv1199c, (MTCI364.11c), len: 415 aa. Possible transposase for IS1081, identical to TRA1_MYCBO|P35882 transposase for insertion sequence element (415 aa); region identical to MTCY441.35 (100.0% identity in 261 aa overlap); and almost identical to MTCY10G2.02c (415 aa) (99.8% identity in 415 aa overlap). Contains PS01007 Transposases, Mutator family, signature, PS00435 Peroxidases proximal heme-ligand signature. P60230 No insertion seqs and phages P60230 P60230 GO:0004803,GO:0006313,GO:0003677 P60230 NC_000962.3 Mycobrowser_v5 CDS 1345260 1346324 . + 0 Rv1202 dapE Involved in biosynthesis of diaminopimelate and lysine from aspartate semialdehyde (at the fifth step) [catalytic activity: N-succinyl-ll-2,6-diaminoheptanedioate + H2O = succinate + ll-2,6-diaminoheptanedioate] Probable succinyl-diaminopimelate desuccinylase DapE Rv1202, (MTCI364.14), len: 354 aa. Probable dapE, succinyl-diaminopimelate desuccinylase, similar to DAPE_CORGL|Q59284 succinyl-diaminopimelate desuccinylase from Corynebacterium glutamicum (369 aa), FASTA scores: opt: 1301, E(): 0, (55.7% identity in 359 aa overlap), highly similar to Q49949|U1756G (400 aa), FASTA scores: opt: 2045, E(): 0, (87.0% identity in 354 aa overlap). P9WHS9 No intermediary metabolism and respiration Q7D8M5 P9WHS9 GO:0005515,GO:0006508,GO:0009014,GO:0008237 3.5.1.18 P9WHS9 NC_000962.3 Mycobrowser_v5 CDS 1349332 1351125 . + 0 Rv1206 fadD6 Function unknown, but supposed involvement in lipid degradation. Probable fatty-acid-CoA ligase FadD6 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv1206, (MTCI364.18), len: 597 aa. Probable fadD6, fatty-acid-CoA synthetase, highly similar to several e.g. NP_251583.1|NC_002516 probable very-long-chain acyl-CoA synthetase from Pseudomonas aeruginosa (608 aa); Q60714 mouse fatty acid transport protein fatp (646 aa), FASTA scores: opt:712, E(): 0, (36.8% identity in 600 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family. O05307 No lipid metabolism O05307 O05307 GO:0008152,GO:0016874 6.2.1.- O05307 NC_000962.3 Mycobrowser_v5 CDS 1355836 1357050 . + 0 Rv1213 glgC Involved in glycogen biosynthesis (first step) [catalytic activity:ATP + alpha-D-glucose 1-phosphate = diphosphate + ADP-glucose]. Glucose-1-phosphate adenylyltransferase GlgC (ADP-glucose synthase) (ADP-glucose pyrophosphorylase) Rv1213, (MTCI364.25), len: 404 aa. glgC, glucose-1-phosphate adenylyltransferase, similar to many e.g. GLGC_ECOLI|P00584 Escherichia coli (430 aa), FASTA scores: opt: 1075, E(): 0, (40.3% identity in 407 aa overlap); highly similar to Q49961 GLGC from Mycobacterium leprae (419 aa), FASTA scores: opt: 2532, E(): 0, (92.6% identity in 404 aa overlap). Belongs to the bacterial and plants glucose-1-phosphate adenylyltransferase family. P9WN43 No intermediary metabolism and respiration P64241 P9WN43 GO:0008878,GO:0005978,GO:0005524 2.7.7.27 P9WN43 NC_000962.3 Mycobrowser_v5 CDS 1360155 1361801 . - 0 Rv1217c Rv1217c Thought to be involved in active transport of tetronasin across the membrane (export): tetronasin resistance by an export mechanism. Responsible for the translocation of the substrate across the membrane. Probable tetronasin-transport integral membrane protein ABC transporter Rv1217c, (MTCI364.29c), len: 548 aa. Probable tetronasin-transport integral membrane ABC transporter (see citation below), similar to many e.g. AL049754|SCH10_12 probable ABC-type transport system membrane-spanning protein from Streptomyces coelicolor (539 aa), FASTA scores: opt: 1309, E(): 0, (40.9% identity in 550 aa overlap); Q54407|X73633 TnrB3 protein from Streptomyces longisporoflavus (337 aa), FASTA scores: opt: 692, E(): 0, (39.5% identity in 324 aa overlap); etc. Also has regions similar to Mycobacterium leprae proteins Q49964|U1756Q (109 aa), FASTA scores: opt: 431, E(): 3.1e-20, (64.8% identity in 105 aa overlap) and Q49965|U1756R (82 aa), FASTA scores: opt:154, E(): 0.0028, (61.0% identity in 41 aa overlap). O05318 No cell wall and cell processes O05318 O05318 NC_000962.3 Mycobrowser_v5 CDS 1364413 1365186 . + 0 Rv1221 sigE The sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released. Seems to be regulated by sigh (Rv3223c product). Seems to regulate the heat-shock response. Alternative RNA polymerase sigma factor SigE Rv1221, (MTCI61.04), len: 257 aa. SigE, alternative sigma factor of extracytoplasmic function (ECF) family (see citations below). Similar to many e.g. RPOE_HAEIN|P44790 RNA polymerase sigma-e factor from Haemophilus influenzae (189 aa), FASTA scores: opt: 247, E(): 3.4e-06, (28.5% identity in 186 aa overlap); etc. Also similar to MTCY07D11.03 rpoE from Mycobacterium tuberculosis (35.2% identity in 159 aa overlap). Belongs to the sigma-70 factor family, ECF subfamily. Three promoters and three translational start codons have been detected (See Dona et al., 2008). Fourth transcriptional start point has been identified (See Pang et al., 2007). Note that in Mycobacterium bovis BCG, the sigE gene is transcribed from two promoters, P1 and P2, and that these promoters were expressed at temperatures from 30-50 degrees Celsius. P9WGG7 No information pathways O06289 P9WGG7 GO:0016987,GO:0003700,GO:0006352,GO:0006355 P9WGG7 NC_000962.3 Mycobrowser_v5 CDS 1365875 1367461 . + 0 Rv1223 htrA Possibly hydrolyzes peptides and/or proteins (seems to cleave preferentially after serine residue). Probable serine protease HtrA (DEGP protein) Rv1223, (MTCI61.06), len: 528 aa. Probable htrA (alternate gene name: degP), serine protease precursor (see citations below), equivalent to U15180|MLU15180_31|Q49972|ML1078|HTRA possible serine protease from Mycobacterium leprae (533 aa), FASTA scores: opt: 2777, E(): 4.1e-141, (81.6% identity in 533 aa overlap). Also similar to many others e.g. HTRA_ECOLI|P09376 protease do precursor from Escherichia coli (474 aa), FASTA scores: opt: 581, E(): 9.1e-27, (36.3% identity in 278 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Start changed since first submission (-21 aa). Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). O06291 No intermediary metabolism and respiration O06291 O06291 GO:0006508,GO:0006950,GO:0004252,GO:0005515 3.4.21.- O06291 NC_000962.3 Mycobrowser_v5 CDS 1367891 1368721 . - 0 Rv1225c Rv1225c Function unknown Conserved hypothetical protein Rv1225c, (MTCI61.08c), len: 276 aa. Conserved hypothetical protein, some similarity to other hypothetical proteins e.g. AE001078|AE001078_2 Archaeoglobus fulgidus (265 aa), FASTA scores: opt: 339, E(): 5.1e-15, (27.1% identity in 262 aa overlap), and to NAGD_ECOLI|P15302 nagd protein from Escherichia coli (250 aa), FASTA scores: opt: 167, E(): 6.4e-12, (24.8% identity in 258 aa overlap). Also weakly similar to Mycobacterium tuberculosis hypothetical protein Rv3400|MTCY78.28c (29.1% identity in 251 aa overlap). O33221 No conserved hypotheticals O33221 O33221 GO:0008152,GO:0016787 O33221 NC_000962.3 Mycobrowser_v5 CDS 1374861 1376168 . - 0 Rv1232c Rv1232c Function unknown Conserved protein Rv1232c, (MTV006.04c), len: 435 aa. Conserved protein, similar to other hypothetical proteins e.g. AB013374|AB013374_2 Bacillus halodurans C-125 mamX (449 aa), FASTA scores: opt: 381, E(): 1e-16, (29.9% identity in 251 aa overlap). Some similarity in N-terminus to U15180|MLU1518033 hypothetical Mycobacterium leprae protein u1756u (329 aa), FASTA scores: opt: 300, E(): 4.1e-12, (69.3% identity in 75 aa overlap). O86315 No conserved hypotheticals O86315 O86315 O86315 NC_000962.3 Mycobrowser_v5 CDS 1377524 1378930 . + 0 Rv1235 lpqY Thought to be involved in active transport of sugar across the membrane (import). Probable sugar-binding lipoprotein LpqY Rv1235, (MTV006.07), len: 468 aa. Probable lpqY, sugar-binding lipoprotein component of sugar transport system (see citation below), equivalent to MLU1518034 protein u1756v from Mycobacterium leprae (469 aa), FASTA scores: opt: 2442, E(): 0, (77.4% identity in 470 aa overlap). Also similar to P18815|MALE_ENTAE maltose-binding periplasmic protein from Enterobacter aerogenes (396 aa), FASTA scores: opt: 193, E(): 2.3e-05, (24.2% identity in 297 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. P9WGU9 No cell wall and cell processes Q7D8J9 P9WGU9 GO:0005215,GO:0006810 P9WGU9 NC_000962.3 Mycobrowser_v5 CDS 1380684 1381865 . + 0 Rv1238 sugC Involved in active transport of sugar across the membrane (import). Responsible for energy coupling to the transport system. Probable sugar-transport ATP-binding protein ABC transporter SugC Rv1238, (MTV006.10), len: 393 aa. Probable sugC, sugar-transport ATP-binding protein ABC transporter (see citation below). Highly similar to U15180 protein ugpC from Mycobacterium leprae (392 aa), FASTA score: opt: 2007, E(): 0, (79.9% identity in 389 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). P9WQI3 No cell wall and cell processes O50454 P9WQI3 GO:0043190,GO:0016887,GO:0016820,GO:0006810,GO:0005524 P9WQI3 NC_000962.3 Mycobrowser_v5 CDS 1484279 1484959 . + 0 Rv1321 Rv1321 Function unknown Conserved hypothetical protein Rv1321, (MTCY130.06), len: 226 aa. Conserved hypothetical protein. Equivalent to P53524|YD21_MYCLE hypothetical protein from Mycobacterium leprae (201 aa), FASTA scores: opt: 1144, E(): 0, (87.6% identity in 193 aa overlap). Some similarity to hypothetical proteins from other organisms e.g. Y225_METJA|Q57678 Methanococcus jannaschii (263 aa), FASTA scores: E(): 6.5e-05, (25.0% identity in 212 aa overlap). P9WIY5 No conserved hypotheticals Q10634 P9WIY5 P9WIY5 NC_000962.3 Mycobrowser_v5 CDS 1387798 1388628 . - 0 Rv1245c Rv1245c Function unknown; supposedly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv1245c, (MTV006.17c), len: 276 aa. Probable short-chain dehydrogenase/reductase, equivalent to NP_301801.1|NC_002677 short chain alcohol dehydrogenase from Mycobacterium leprae (277 aa). Also highly similar to various dehydrogenases and oxidoreductases e.g. NP_250228.1|NC_002516 probable short-chain dehydrogenase from Pseudomonas aeruginosa (295 aa); NP_421969.1|NC_002696 short chain dehydrogenase family protein from Caulobacter crescentus (278 aa); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. Rv3085|MTV013.06 probable short-chain type dehydrogenase/reductase (276 aa), FASTA scores: opt: 368, E(): 1.2e-16, (35.3% identity in 224 aa overlap); Rv3057c|MTCY22D7.24 putative short chain alcohol dehydrogenase/reductase (287 aa), FASTA scores: opt: 471, E(): 1.3e-21, (32.4% identity in 281 aa overlap); etc. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. O50460 No intermediary metabolism and respiration O50460 O50460 GO:0055114,GO:0016491 1.-.-.- O50460 NC_000962.3 Mycobrowser_v5 CDS 1389357 1393052 . - 0 Rv1248c Rv1248c Involved in cellular metabolism. Has alpha-ketoglutarate dehydrogenase (KDH) [catalytic activity: 2-oxoglutarate + lipoamide = S-succinyldihydrolipoamide + CO2], alpha-ketoglutarate decarboxylase (KGD) [catalytic activity: 2-oxoglutarate = succinate semialdehyde + CO2], and 2-hydroxy-3-oxoadipate (HOA) synthase [catalytic activity: 2-oxoglutarate + glyoxylate + H+ = 2-hydroxy-3-oxoadipate + CO2] activities. Multifunctional alpha-ketoglutarate metabolic enzyme Rv1248c, (MTV006.20c), len: 1231 aa. Multifunctional alpha-ketoglutarate metabolic enzyme, highly similar to D84102 Corynebacterium glutamicum (1257 aa), FASTA scores: opt: 4418, E(): 0, (59.4% identity in 1223 aa overlap). Cofactor: thiamine diphosphate. Start changed since first submission (+17 aa). P9WIS5 No intermediary metabolism and respiration O50463 P9WIS5 GO:0008415,GO:0006096,GO:0004591,GO:0030976,GO:0006099,GO:0008683 4.1.1.71 P9WIS5 NC_000962.3 Mycobrowser_v5 CDS 1406081 1407340 . - 0 Rv1258c Rv1258c Thought to be involved in transport of undetermined substrate (possibly macrolide) across the membrane (export). Responsible for the translocation of the undetermined substrate across the membrane. Probable conserved integral membrane transport protein Rv1258c, MTCY50.24, len: 419 aa. Probable conserved integral membrane transport (efflux) protein, possibly member of major facilitator superfamily (MFS), highly similar to O32859|tap protein multidrug-resistance efflux pump from Mycobacterium fortuitum (409 aa), FASTA scores: E(): 0, (68.4% identity in 408 aa overlap). Contains PS00216 Sugar transport proteins signature 1. P9WJX9 No cell wall and cell processes P64783 P9WJX9 GO:0005886,GO:0046677,GO:0055085,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1408240 1409358 . + 0 Rv1260 Rv1260 Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv1260, (MTCY50.22c), len: 372 aa. Probable oxidoreductase, highly similar to E1245747|AL021411 putative oxidoreductase SC7H1.18 from Streptomyces coelicolor (397 aa), FASTA scores: E(): 1.4e-29, (45.9% identity in 355 aa overlap); also some similarity to G912582 FAD binding protein homologue from Pseudomonas aeruginosa (286 aa), FASTA scores: opt: 245, E(): 2e-09, (27.5% identity in 251 aa overlap); PCPB_FLASP|P42535 pentachlorophenol 4-monooxygenase (537 aa), FASTA scores: opt: 219, E(): 1.7e-07, (23.3% identity in 360 aa overlap); TETX_BACFR|Q01911 tetracycline resistance protein (388 aa), FASTA scores: opt: 183, E(): 3e-05, (22.8% identity in 373 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins Rv0575c and Rv1751. P9WM51 No intermediary metabolism and respiration Q11058 P9WM51 GO:0055114,GO:0004497 P9WM51 NC_000962.3 Mycobrowser_v5 CDS 1411894 1413087 . + 0 Rv1264 Rv1264 Possibly involved in cAMP synthesis [catalytic activity: ATP = 3',5'-cyclic AMP + diphosphate]. Activity is PH-dependent. Inhibited by polyphosphates. Adenylyl cyclase (ATP pyrophosphate-lyase) (adenylate cyclase) Rv1264, (MTCY50.18c), len: 397 aa. Adenylate cyclase (function proven experimentally: see Linder et al., 2002), showing some similarity to other adenylate cyclases e.g. CYAA_BRELI|P27580 (403 aa), FASTA scores, opt: 270, E(): 1.3e-10, (29.3% identity in 317 aa overlap); etc. Similar to other putative cyclases in M. tuberculosis e.g. Rv2212, Rv1647. The C terminus seems to code for a catalytic domain belonging to a subfamily of adenylyl cyclase isozymes (mostly found in Gram-positive bacteria). The N terminus seems to be a potential novel regulator of adenylyl cyclase activity (autoinhibitory domain). Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. P9WMU9 No intermediary metabolism and respiration 1Y10,1Y11,2EV1,2EV2,2EV3,2EV4 Q11055 P9WMU9 GO:0004016,GO:0006171,GO:0008289,GO:0046872,GO:0030818,GO:0042803,GO:0005524 4.6.1.1 P9WMU9 NC_000962.3 Mycobrowser_v5 CDS 1484982 1485278 . + 0 Rv1322 Rv1322 Function unknown Conserved hypothetical protein Rv1322, (MTCY130.07), len: 98 aa. Conserved hypothetical protein. P9WM27 No conserved hypotheticals P9WM27 NC_000962.3 Mycobrowser_v5 CDS 1422302 1424050 . - 0 Rv1273c Rv1273c Thought to be involved in active transport of drugs across the membrane (export): multidrugs resistance by an export mechanism. Responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane. Probable drugs-transport transmembrane ATP-binding protein ABC transporter Rv1273c, (MTCY50.09), len: 582 aa. Probable drugs-transport transmembrane ATP-binding protein ABC transporter (see citation below), similar to e.g. YWJA_BACSU|P45861 hypothetical abc transporter from B. subtilis (575 aa), FASTA scores: opt: 810, E(): 0, (27.0% identity in 578 aa overlap); etc. Contains PS00136 Serine proteases, subtilase family, aspartic acid active site; 2 x PS00211 ABC transporters family signature; and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters), MSBA subfamily. P9WQJ1 No cell wall and cell processes P0A4W4 P9WQJ1 GO:0042626,GO:0016021,GO:0005886,GO:0055085,GO:0005524 P9WQJ1 NC_000962.3 Mycobrowser_v5 CDS 1431665 1433440 . - 0 Rv1280c oppA Involved in active transport of oligopeptide across the membrane (import). This protein is a component of the oligopeptide permease, a binding protein-dependent transport system; it binds peptides up to five amino acids long with high affinity. Probable periplasmic oligopeptide-binding lipoprotein OppA Rv1280c, (MTCY50.02), len: 591 aa. Probable oppA, oligopeptide-binding lipoprotein component of peptide transport system (see citation below), sharing some similarity to other periplasmic solute binding proteins e.g. OPPA_SALTY|P06202 periplasmic oligopeptide-binding protein from Salmonella typhimurium (542 aa), FASTA scores: E(): 5.1e-05, (22.1% identity in 458 aa overlap); etc. Also similar to Rv1166 and Rv2585c from Mycobacterium tuberculosis. Has possible N-terminal signal sequence and prokaryotic lipoprotein lipid attachment site (PS00013). Belongs to the bacterial extracellular solute-binding protein family 5. P9WGU5 No cell wall and cell processes P66771 P9WGU5 GO:0005886,GO:0006810,GO:0005215,GO:0016021 P9WGU5 NC_000962.3 Mycobrowser_v5 CDS 1437909 1438907 . + 0 Rv1285 cysD Involved in sulfate activation pathway. First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway [catalytic activity:ATP + sulfate = diphosphate + adenylylsulfate] Probable sulfate adenylyltransferase subunit 2 CysD Rv1285, (MTCY373.04), len: 332 aa. Probable cysD, sulfate adenylyltransferase subunit 2 (see Wooff et al., 2002), homology suggests start site at aa 24 or 28, similar to e.g. CYSD_ECOLI|P21156 sulfate adenylate transferase subunit 2 from Escherichia coli (302 aa), FASTA score: opt: 973, E():0, (52.5% identity in 303 aa overlap). Also similar to Mycobacterium tuberculosis Rv2392, 3'-phosphoadenylylsulfate reductase. Belongs to the PAPS reductase family. CYSD subfamily. Thought to be differentially expressed within host cells (see Triccas et al., 1999). P9WIK1 No intermediary metabolism and respiration P65670 P9WIK1 GO:0004781,GO:0000103,GO:0019419,GO:0005524 2.7.7.4 P9WIK1 NC_000962.3 Mycobrowser_v5 CDS 2413348 2414922 . - 0 Rv2154c ftsW Unknown FtsW-like protein FtsW Rv2154c, (MTCY270.14), len: 524 aa. Probable ftsW, cell division protein, related to MTCY10H4.17c, 3.2e-17. FASTA best: SP5E_BACSU P07373 stage V sporulation protein E (366 aa) opt: 755, E(): 1.6e-33; (38.4% identity in 357 aa overlap). FtsW|Rv2154c interacts with PbpB|Rv2163c and FtsZ|RvRv2150c (See Datta et al., 2006). P9WN97 No cell wall and cell processes P63762 P9WN97 GO:0051301,GO:0016021,GO:0009252,GO:0005886,GO:0007049 NC_000962.3 Mycobrowser_v5 CDS 2900226 2900690 . - 0 Rv2576c Rv2576c Unknown Possible conserved membrane protein Rv2576c, (MTCY227.25), len: 154 aa. Possible conserved membrane protein, showing similarity with Q9ZFC2 hypothetical 15.7 KDA protein from Mycobacterium sp. FM10 (146 aa), FASTA scores: opt: 235, E(): 4.1e-08, (31.35% identity in 150 aa overlap). P9WL83 No cell wall and cell processes P9WL83 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1449375 1450700 . + 0 Rv1294 thrA Involved in the conversion of L-aspartate to homoserine (third step). Homoserine participates in the biosynthesis of threonine and then isoleucine and in the biosynthesis of methionine [catalytic activity: L-homoserine + NAD(P)(+) = L-aspartate 4-semialdehyde + NAD(P)H.] Probable homoserine dehydrogenase ThrA Rv1294, (MTCY373.14), len: 441 aa. Probable thrA (hom), homoserine dehydrogenase, highly similar to DHOM_MYCLE|P46806 from Mycobacterium leprae (441 aa), FASTA scores: opt: 2437, E():0, (89.5% identity in 438 aa overlap). Contains PS00017 ATP/GTP-binding site motif A; PS01042 Homoserine dehydrogenase signature. Belongs to the homoserine dehydrogenase family. P9WPX1 No intermediary metabolism and respiration P63629 P9WPX1 GO:0016597,GO:0004412,GO:0009097,GO:0009086,GO:0055114,GO:0009088,GO:0050661 1.1.1.3 P9WPX1 NC_000962.3 Mycobrowser_v5 CDS 1453204 1455012 . + 0 Rv1297 rho Facilitates transcription termination by a mechanism that involves rho binding to the nascent RNA, activation of rho'S RNA-dependent ATPase activity, and release of the mRNA from the DNA template Probable transcription termination factor Rho homolog Rv1297, (MTCY373.17), len: 602 aa. Probable rho, transcription termination factor homolog, highly similar to many e.g. RHO_MYCLE|P45835 Mycobacterium leprae (610 aa), FASTA scores: (81.5% identity in 612 aa overlap). Contains 1 RNA recognition motif (RRM). Nucleotide position 1453608 in the genome sequence has been corrected, T:C resulting in G135G. P9WHF3 No information pathways P66028 P9WHF3 GO:0015986,GO:0003723,GO:0004386,GO:0016820,GO:0033178,GO:0045449,GO:0006353,GO:0003715,GO:0005524 3.6.4.- P9WHF3 NC_000962.3 Mycobrowser_v5 CDS 1458295 1459509 . + 0 Rv1302 rfe Thought to be involved in AG biosynthesis. May be the tunicamycin sensitive transferase that catalyzes the synthesis of GlcNAc-pyrophosphorylundecaprenol (lipid I), the first lipid-linked intermediate involved in ECA synthesis. Probable undecapaprenyl-phosphate alpha-N-acetylglucosaminyltransferase Rfe (UDP-GlcNAc transferase) Rv1302, (MTCY373.22), len: 404 aa. Probable rfe (alternate gene name: wecA), undecaprenyl-phosphate alpha-N-acetylglucosaminyltransferase (see citation below), equivalent to RFE_MYCLE|P45830 Mycobacterium leprae (398 aa), FASTA scores, opt: 2285, E(): 0, (89.2% identity in 398 aa overlap). P9WMW5 No cell wall and cell processes Q10606 P9WMW5 GO:0009103,GO:0008963,GO:0005886,GO:0016757,GO:0016021 2.7.8.n2 P9WMW5 NC_000962.3 Mycobrowser_v5 CDS 1461843 1463183 . + 0 Rv1307 atpH This protein seems to be part of the stalk that LINKS cf(0) to cf(1). It either transmits conformational changes from cf(0) into cf(1) or is implicated in proton conduction [catalytic activity: ATP + H(2)O + H(+)(in) = ADP + phosphate + H(+)(out)] Probable ATP synthase delta chain AtpH Rv1307, (MTCY373.27), len: 446 aa. Probable atpH, ATP synthase delta chain. This protein is much longer than that of other bacterial delta chains, the C-terminal region is homologous to delta chains while the N-terminal region is similar to B/B' subunits e.g. ATPD_STRLI|P50008 ATP synthase delta chain from Streptomyces lividans (273 aa), FASTA scores: opt: 505, E(): 5.4e-23, (35.0% identity in 277 aa overlap); and ATPF_HAEIN|P43720 ATP synthase B chain from Haemophilus influenzae (156 aa), FASTA scores: opt: 216, E(): 1.2e-06, (26.1% identity in 153 aa overlap). subunit: F-type ATPases have 2 components, cf(1) - the catalytic core - and cf(0) - the membrane proton channel. cf(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). cf(0) has three main subunits: A, B and C. Belongs to the ATPase delta chain family. P9WPV3 No intermediary metabolism and respiration P0A500 P9WPV3 GO:0003824,GO:0046933,GO:0016021,GO:0005886,GO:0045261,GO:0045263,GO:0015986 P9WPV3 NC_000962.3 Mycobrowser_v5 CDS 1463228 1464877 . + 0 Rv1308 atpA Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit [catalytic activity:ATP + H(2)O + H(+)(in) = ADP + phosphate + H(+)(out)] Probable ATP synthase alpha chain AtpA Rv1308, (MTCY373.28), len: 549 aa. Probable atpA, ATP synthase alpha chain, highly similar to ATPA_MYCLE|P45825 from Mycobacterium leprae (558 aa), FASTA scores: opt: 3233, E(): 0, (90.3% identity in 547 aa overlap). Contains PS00017 ATP/GTP-binding site motif A, PS00152 ATP synthase alpha and beta subunits signature. subunit: F-type ATPases have 2 components, cf(1) - the catalytic core - and cf(0) - the membrane proton channel. cf(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). cf(0) has three main subunits: A, B and C. Belongs to the ATPase alpha/beta chains family. P9WPU7 No intermediary metabolism and respiration P63673 P9WPU7 GO:0046933,GO:0005886,GO:0042777,GO:0045261,GO:0046961,GO:0005524 3.6.3.14 P9WPU7 NC_000962.3 Mycobrowser_v5 CDS 1468171 1469505 . - 0 Rv1313c Rv1313c Possibly required for the transposition of the insertion element IS1557. Possible transposase Rv1313c, (MTCY373.33c), len: 444 aa. Possible IS1557 transposase, similar to several transposases e.g. U57649|DBU57649 ORF1 from dibenzofuran-degrading bacterium DPO360 (163 aa), FASTA scores: opt: 767, E(): 0, (67.3% identity in 168 aa overlap); TNPA_BORPA|Q06126 transposase for insertion sequence element IS1001 from Bordetella parapertussis (406 aa), FASTA scores: opt: 254, E(): 3.3e-10, (24.9% identity in 402 aa overlap). Also similar to putative Mycobacterium tuberculosis transposases, Rv3798 and Rv0741. P9WKH7 No insertion seqs and phages Q10621 P9WKH7 GO:0004803,GO:0006313,GO:0003677 P9WKH7 NC_000962.3 Mycobrowser_v5 CDS 1477628 1479118 . - 0 Rv1317c alkA Involved in the adaptive response to alkylation damage in DNA caused by alkylating agents. Repairs the SP diastereomer of DNA methylphosphotriester lesions by a direct and irreversible transfer of the methyl group to one of its own cysteine residues. The methylated ALKA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. Probable bifunctional regulatory protein and DNA repair enzyme AlkA (regulatory protein of adaptative response) (methylphosphotriester-DNA--protein-cysteine S-methyltransferase) Rv1317c, (MTCY130.02c), len: 496 aa. Probable alkA (alternate gene name: ada), bifunctional regulatory protein (see citation below) and DNA repair enzyme, similar to 3MG2_ECOLI|P04395 dna-3-methyladenine glycosidase II from Escherichia coli (282 aa), FASTA scores, opt: 437, E(): 8.6e-22, (32.8% identity in 293 aa overlap), also similar to other ada proteins e.g. ADA_SALTY|P26189 Salmonella typhimurium (352 aa), FASTA scores: E(): 5.3e-08, (35.9% identity in 156 aa overlap). Contains PS00041 Bacterial regulatory proteins, araC family signature. P9WJW3 No information pathways Q10630 P9WJW3 GO:0006284,GO:0003908,GO:0006355,GO:0006350,GO:0008270,GO:0003677 P9WJW3 NC_000962.3 Mycobrowser_v5 CDS 1480894 1482501 . - 0 Rv1319c Rv1319c Thought to play an essential role in regulation of cellular metabolism by catalysing the synthesis of a second messenger, camp [catalytic activity: ATP = 3',5'-cyclic AMP + pyrophosphate]. Possible adenylate cyclase (ATP pyrophosphate-lyase) (adenylyl cyclase) Rv1319c, (MTCY130.04c), len: 535 aa. Possible adenylate cyclase. Some similarity at the C-terminus to CYAA_RHIME|P19485 adenylate cyclase from Rhizobium meliloti (193 aa), FASTA scores: opt: 254, E(): 2.4e-10, (33.3% identity in 144 aa overlap); similar to other mycbacterium tuberculosis putative adenylate cyclases e.g. Rv1318c|MTCY130.03c (541 aa), FASTA scores: opt: 2505, E(): 0, (71.0% identity in 534 aa overlap); Rv1320c|MTCY130.05c (567 aa), FASTA scores: opt: 2354, E(): 0, (66.3% identity in 534 aa overlap). N-terminus is hydrophobic. Belongs to adenylyl cyclase class-3 family. P9WQ31 No intermediary metabolism and respiration P0A4Y2 P9WQ31 GO:0016021,GO:0016849,GO:0005886,GO:0004871,GO:0007165,GO:0009190 P9WQ31 NC_000962.3 Mycobrowser_v5 CDS 1488154 1489965 . - 0 Rv1325c PE_PGRS24 Function unknown PE-PGRS family protein PE_PGRS24 Rv1325c, (MTCY130.10c), len: 603 aa. PE_PGRS24, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of ala-, gly-rich proteins (see Brennan & Delogu 2002), similar to many e.g. YQ04_MYCTU|P71933 hypothetical 63.1 kDa glycine-rich protein (778 aa), FASTA scores: E(): 0, (52.3% identity in 724 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). P9WIF7 No PE/PPE Q10637 P9WIF7 P9WIF7 NC_000962.3 Mycobrowser_v5 CDS 1499213 1500559 . - 0 Rv1330c pncB1 Involved in NAD salvage. Phosphoribosylation of nicotinic acid. [catalytic activity: nicotinate + 5-phosphoribosyl 1-pyrophosphate = nicotinate mononucleotide + diphosphate] Nicotinic acid phosphoribosyltransferase PncB1 Rv1330c, (MTCY130.15c), len: 448 aa. PncB1, nicotinic acid phosphoribosyltransferase (See Boshoff et al., 2008). Similar to e.g. O32090 YUEK protein from Bacillus subtilis (490 aa), FASTA scores: E(): 8.6e-22, (37.9% identity in 369 aa overlap). Also similar to Mycobacterium tuberculosis Rv0573c|MTV039.11c (38.0% identity in 437 aa overlap). Start changed since original submission based on similarity; previous start at position 1500740 (-61 aa). P9WJI9 No intermediary metabolism and respiration Q10641 P9WJI9 GO:0019363,GO:0004516 2.4.2.11 P9WJI9 NC_000962.3 Mycobrowser_v5 CDS 1509281 1510846 . + 0 Rv1345 mbtM Thought to be involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins Probable fatty acyl-AMP ligase MbtM Rv1345, (MTCY02B10.09), len: 521 aa. Probable mbtM, fatty acyl-AMP ligase. Similar to N-terminus of T34918 polyketide synthase from Streptomyces coelicolor (2297 aa); and PKSJ_BACSU|P40806 putative polyketide biosynthesis protein from Bacillus subtilis (557 aa), FASTA scores: opt: 537, E(): 8.2e-27, (27.1% identity in 468 aa overlap). Also similar to other proteins from Mycobacterium tuberculosis eg Rv1013|MTCI237.30|MTCY10G2.36c|pks16 putative polyketide synthase (544 aa); etc. Note that previously known as fadD33. P9WQ41 No lipid metabolism P0A4X8 P9WQ41 GO:0006826,GO:0008922,GO:0008152,GO:0005524 6.2.1.20 P9WQ41 NC_000962.3 Mycobrowser_v5 CDS 1513047 1515626 . + 0 Rv1348 irtA Involved in iron homeostasis. Responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane. Iron-regulated transporter IrtA Rv1348, (MTCY02B10.12), len: 859 aa. IrtA, iron-regulated transporter. Probable transmembrane protein, similar to HMT1_SCHPO|Q02592 heavy metal tolerance protein precursor from Schizosaccharomyces pombe (830 aa), FASTA scores: opt: 806, E(): 5.1e-39, (32.9% identity in 504 aa overlap); etc. Also similar to MTCY02B10.13 from Mycobacterium tuberculosis, FASTA score: (31.9% identity in 576 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). Cofactor: FAD P9WQJ9 No cell wall and cell processes P63391 P9WQJ9 GO:0042626,GO:0016021,GO:0055114,GO:0016491,GO:0005886,GO:0055085,GO:0005524 P9WQJ9 NC_000962.3 Mycobrowser_v5 CDS 1515623 1517362 . + 0 Rv1349 irtB Involved in iron homeostasis. Responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane. Iron-regulated transporter IrtB Rv1349, (MTCY02B10.13), len: 579 aa. IrtB, iron-regulated transporter. Probable transmembrane protein, most similar to YWJA_BACSU|P45861 hypothetical ABC transporter from Bacillus subtilis (575 aa), FASTA scores: opt: 721, E(): 1.8e-35, (28.9% identity in 567 aa overlap); etc. Also similar to MTCY02B10.12 from Mycobacterium tuberculosis, FASTA score: (31.9% identity in 576 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). Predicted possible vaccine candidate (See Zvi et al., 2008). P9WQJ7 No cell wall and cell processes P63393 P9WQJ7 GO:0042626,GO:0016021,GO:0005886,GO:0055085,GO:0005524 P9WQJ7 NC_000962.3 Mycobrowser_v5 CDS 1520005 1521876 . - 0 Rv1354c Rv1354c Function unknown Conserved hypothetical protein Rv1354c, (MTCY02B10.18c), len: 623 aa. Conserved hypothetical protein, similar to many hypothetical proteins e.g. the C-terminus of G1001455 Synechocystis sp. (1244 aa), FASTA scores: opt: 933, E(): 0, (36.8% identity in 462 aa overlap); also similar to Rv1357c|MTCY02B10.21c (34.0% identity in 253 aa overlap). P9WM13 No conserved hypotheticals P64825 P9WM13 P9WM13 NC_000962.3 Mycobrowser_v5 CDS 1526612 1530091 . + 0 Rv1358 Rv1358 Involved in transcriptional mechanism Probable transcriptional regulatory protein Rv1358, (MTCY02B10.22), len: 1159 aa. Probable transcriptional regulatory protein, some similarity to AFSR_STRCO|P25941 regulatory protein afsr from Streptomyces coelicolor (993 aa), FASTA scores: opt: 210, E(): 5.5e-06, (27.5% identity in 739 aa overlap). Similar also to Rv0890C|MTCY31.18c (65.5% identity in 884 aa overlap) and to Rv1359|MTCY02B10.23 (43.7% identity in 197 aa overlap). Contains PS00017 ATP/GTP-binding site motif A, PS00622 Bacterial regulatory proteins, luxR family signature. Helix turn helix motif present at aa 1116-1137, (Score 1291, +3.59 SD). Q11028 No regulatory proteins Q11028 Q11028 GO:0005622,GO:0016849,GO:0006355,GO:0043565,GO:0003700,GO:0000156,GO:0000160,GO:0009190 Q11028 NC_000962.3 Mycobrowser_v5 CDS 1541020 1541805 . + 0 Rv1368 lprF Unknown Probable conserved lipoprotein LprF Rv1368, (MTCY02B12.02), len: 261 aa. Probable lprF, conserved lipoprotein; similar to Mycobacterium tuberculosis hypothetical lipoproteins e.g. Rv1270c|Y08C_MYCTU|Q11049 hypothetical 26.4 kDa protein cy50.12. (257 aa), FASTA scores: opt: 286, E(): 5.3e-11, (26.3% identity in 270 aa overlap), also Rv1411c|MTCY21B4.28c, (32.8% identity in 253 aa overlap) and Rv2945c. Contains possible N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein lipid attachment site (PS00013). Belongs to the LPPX/lprafg family of lipoproteins. P9WK47 No cell wall and cell processes P65314 P9WK47 GO:0005886 P9WK47 NC_000962.3 Mycobrowser_v5 CDS 1550579 1551217 . - 0 Rv1377c Rv1377c Function unknown; probably involved in cellular metabolism Putative transferase Rv1377c, (MTCY02B12.11c), len: 212 aa. Putative transferase, similar to YQEM_BACSU|P54458 hypothetical 28.3 kDa protein from Bacillus subtilis (247 aa), FASTA scores: opt: 221, E(): 7.6e-08, (30.6% identity in 144 aa overlap); some similarity to methyltransferases, also similar to Mycobacterium tuberculosis hypothetical proteins Rv0560c, Rv3699, and Rv2675c (~ 39.1% identity in 197 aa overlap). P71805 No intermediary metabolism and respiration P71805 P71805 GO:0008168,GO:0008152 2.-.-.- P71805 NC_000962.3 Mycobrowser_v5 CDS 1551228 1552655 . - 0 Rv1378c Rv1378c Function unknown Conserved hypothetical protein Rv1378c, (MTCY02B12.12c), len: 475 aa. Conserved hypothetical protein, similar to other Mycobacterium tuberculosis hypothetical proteins e.g. Rv3074|MTCY22D7.07C (424 aa), FASTA scores: E(): 0, (73.0% identity in 429 aa overlap). P71806 No conserved hypotheticals P71806 NC_000962.3 Mycobrowser_v5 CDS 1553232 1554191 . + 0 Rv1380 pyrB Involved in pyrimidine biosynthesis (second step) [catalytic activity: carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate] Probable aspartate carbamoyltransferase PyrB (ATCase) (aspartate transcarbamylase) Rv1380, (MTCY02B12.14), len: 319 aa. Probable pyrB, aspartate carbamoyltransferase, similar to many e.g. PYRB_BACCL|P41008 aspartate carbamoyltransferase from Bacillus caldolyticus (308 aa), FASTA scores, opt: 639, E(): 7.3e-36, (39.5% identity in 311 aa overlap). Contains PS00097 Aspartate and ornithine carbamoyltransferases signature. Belongs to the ATCases/OTCases family. P9WIT7 No intermediary metabolism and respiration P65613 P9WIT7 GO:0016597,GO:0004070,GO:0006520,GO:0006221,GO:0006207 2.1.3.2 P9WIT7 NC_000962.3 Mycobrowser_v5 CDS 1555971 1557101 . + 0 Rv1383 carA Involved in both arginine and pyrimidine biosynthesis [catalytic activity: 2 ATP + L-glutamine + CO(2) + H(2)O = 2 ADP + phosphate + glutamate + carbamoyl phosphate] Probable carbamoyl-phosphate synthase small chain CarA (carbamoyl-phosphate synthetase glutamine chain) Rv1383, (MTCY02B12.17), len: 376 aa. Probable carA, Carbamoyl-phosphate synthase small chain, similar to many e.g. CARA_ECOLI|P00907 carbamoyl-phosphate synthase small chain from Escherichia coli (382 aa), FASTA scores: opt: 796, E(): 0, (45.5% identity in 382 aa overlap). Contains PS00442 Glutamine amidotransferases class-I active site. The gatase domain belongs to type-1 glutamine amidotransferases. subunit: composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. P9WPK5 No intermediary metabolism and respiration P71811 P9WPK5 GO:0006526,GO:0004088,GO:0006541,GO:0006221,GO:0005524 6.3.5.5 P9WPK5 NC_000962.3 Mycobrowser_v5 CDS 1557101 1560448 . + 0 Rv1384 carB Involved in both arginine and pyrimidine biosynthesis [catalytic activity: 2 ATP + L-glutamine + CO(2) + H(2)O = 2 ADP + phosphate + glutamate + carbamoyl phosphate.] Probable carbamoyl-phosphate synthase large chain CarB (carbamoyl-phosphate synthetase ammonia chain) Rv1384, (MTCY02B12.18-MTCY21B4.01), len: 1115 aa. Probable carB, Carbamoyl-phosphate synthase large chain , similar to many e.g. CARB_ECOLI|P00968 E. coli (1072 aa), FASTA scores: E(): 0, (52.3% identity in 1118 aa overlap). Contains two PS00867 Carbamoyl-phosphate synthase subdomain signature 2 and PS00866 Carbamoyl-phosphatesynthase subdomain signature 1. subunit: composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. P9WPK3 No intermediary metabolism and respiration P57689 P9WPK3 GO:0006526,GO:0004088,GO:0046872,GO:0006221,GO:0005524 6.3.5.5 P9WPK3 NC_000962.3 Mycobrowser_v5 CDS 1561769 1563388 . + 0 Rv1387 PPE20 Function unknown PPE family protein PPE20 Rv1387, (MTCY21B4.04), len: 539 aa. PPE20, Member of Mycobacterium tuberculosis PPE family of proteins, similar to many e.g. Y05F_MYCTU|Q10892 hypothetical 46.9 kd protein cy251.15 (463 aa), FASTA scores: E(): 4.2e-26, (37.7% identity in 531 aa overlap); similar also to MTCY274.23c (37.5% identity in 168 aa overlap). Contains PS00343 Gram-positive cocci surface proteins 'anchoring' hexapeptide. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WI23 No PE/PPE Q7D8H6 P9WI23 P9WI23 NC_000962.3 Mycobrowser_v5 CDS 1565441 1566697 . + 0 Rv1391 dfp Flavoprotein affecting synthesis of DNA and pantothenate metabolism Probable DNA/pantothenate metabolism flavoprotein homolog Dfp Rv1391, (MTCY21B4.08), len: 418 aa. Probable dfp, DNA/pantothenate metabolism flavoprotein homolog, similar to many e.g. DFP_ECOLI|P24285 Escherichia coli (430 aa), FASTA scores: opt: 763, E(): 0, (40.2% identity in 408 aa overlap). P9WNZ1 No intermediary metabolism and respiration P67733 P9WNZ1 GO:0015937,GO:0015941,GO:0004632,GO:0004633,GO:0010181 6.3.2.5,4.1.1.36 P9WNZ1 NC_000962.3 Mycobrowser_v5 CDS 1566825 1568036 . + 0 Rv1392 metK Involved in the activated methyl cycle. Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme. [catalytic activity : ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine] Probable S-adenosylmethionine synthetase MetK (mat) (AdoMet synthetase) (methionine adenosyltransferase) Rv1392, (MTCY21B4.09), len: 403 aa. Probable metK, S-adenosylmethionine synthetase, similar to many e.g. METK_STAAU|P50307 Staphylococcus aureus (397 aa), FASTA scores: opt: 1484, E(): 0, (58.0% identity in 400 aa overlap). Contains PS00376 S-adenosylmethionine synthetase signature 1, PS00377 S-adenosylmethionine synthetase signature 2. Belongs to the adomet synthetase family. P9WGV1 No intermediary metabolism and respiration 3TDE P77899 P9WGV1 GO:0005737,GO:0000287,GO:0004478,GO:0006730,GO:0005524 2.5.1.6 P9WGV1 NC_000962.3 Mycobrowser_v5 CDS 1571047 1572081 . + 0 Rv1395 Rv1395 Involved in transcriptional mechanism Transcriptional regulatory protein Rv1395, (MTCY21B4.12), len: 344 aa. Transcriptional regulatory protein (see citation below), similar to many e.g. URER_PROMI|Q02458 urease operon transcriptional activator from Proteus mirabilis (293 aa), FASTA scores: E():1.5e-08, (41.7% identity in 84 aa overlap); YHIX_ECOLI|P37639 hypothetical transcriptional regulatory protein from Escherichia coli (274 aa), FASTA scores: opt: 238, E(): 3.5e-09, (27.3% identity in 249 aa overlap); and G296916|X68281 possible virulence-regulating protein from Mycobacterium tuberculosis (339 aa), FASTA scores: opt: 228, E(): 1.9e-08, (27.0% identity in 278 aa overlap). Helix turn helix motif present, aa 261-282 (+4.68 SD). Belongs to the AraC/XylS family of transcriptional regulators. 3' part corrected since first submission (-14 aa). Experimentally shown to induce the expression of the cytochrome P450 gene (Rv1394c) and represses its own transcription. P9WMJ1 No regulatory proteins P68913 P9WMJ1 GO:0006355,GO:0043565,GO:0006350,GO:0003700,GO:0005622 P9WMJ1 NC_000962.3 Mycobrowser_v5 CDS 2752848 2752994 . - 0 Rv2452c Rv2452c Unknown Hypothetical protein Rv2452c, (MTV008.08c), len: 48 aa. Hypothetical unknown protein (see citation below). O53179 No conserved hypotheticals O53179 NC_000962.3 Mycobrowser_v5 CDS 1579598 1580422 . - 0 Rv1403c Rv1403c Causes methylation Putative methyltransferase Rv1403c, (MTCY21B4.20c), len: 274 aa. Putative methyltransferase, similar to PMTA_RHOSH|Q05197 phosphatidylethanolamine m-methyltransferase (203 aa), FASTA scores: opt: 217, E(): 1.1e-07, (37.1% identity in 105 aa overlap); similar to Rv1405c|MTCY21B4.22c (59.3% identity in 273 aa overlap) and to Rv1523, Rv2952, etc. P9WLY9 No intermediary metabolism and respiration P64839 P9WLY9 GO:0008168,GO:0008152 P9WLY9 NC_000962.3 Mycobrowser_v5 CDS 1582166 1583104 . + 0 Rv1406 fmt Modify the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTU-GTP. [catalytic activity: 10-formyltetrahydrofolate + L-methionyl-tRNA + H(2)O = tetrahydrofolate + N-formylmethionyl-tRNA] Probable methionyl-tRNA formyltransferase Fmt Rv1406, (MTCY21B4.23), len: 312 aa. Probable fmt, methionyl-tRNA formyltransferase, similar to many e.g. FMT_ECOLI|P23882 Escherichia coli (314 aa), FASTA scores: opt: 616, E(): 6.7e-31, (39.3% identity in 303 aa overlap). Belongs to the FMT family. P9WND3 No information pathways P64134 P9WND3 GO:0006412,GO:0004479 2.1.2.9 P9WND3 NC_000962.3 Mycobrowser_v5 CDS 1585194 1586213 . + 0 Rv1409 ribG Involved in riboflavin biosynthesis (at the second and third steps). Converts 2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate into 5-amino-6-(ribosylamino)-2,4(1H,3H)-pyrimidinedione 5'-phosphate [catalytic activity 1: 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + H(2)O = 5-amino-6-(5-phosphoribosylamino)uracil + NH(3)] [catalytic activity 2: 5-amino-6-(5-phosphoribitylamino)uracil + NADP(+) = 5-amino-6-(5-phosphoribosylamino)uracil + NADPH]. Probable bifunctional riboflavin biosynthesis protein RibG : diaminohydroxyphosphoribosylaminopyrimidine deaminase (riboflavin-specific deaminase) + 5-amino-6-(5-phosphoribosylamino) uracil reductase (HTP reductase) Rv1409, (MTCY21B4.26), len: 339 aa. Probable ribG (alternate gene name: ribD), bifunctional riboflavin biosynthesis protein, including diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5-amino-6-(5-phosphoribosylamino) uracil reductase, similar to many e.g. RIBD_ECOLI|P25539 riboflavin-specific deaminase from Escherichia coli (367 aa), FASTA scores: E(): 0, (39.8% identity in 364 aa overlap); etc. Contains PS00903 Cytidine and deoxycytidylate deaminases zinc-binding region signature. In the N-terminal section; belongs to the cytidine and deoxycytidylate deaminases family. In the C-terminal section; belongs to the HTP reductase family. P9WPH1 No intermediary metabolism and respiration P71677 P9WPH1 GO:0050661,GO:0008835,GO:0055114,GO:0009231,GO:0008270,GO:0008703 1.1.1.193,3.5.4.26 P9WPH1 NC_000962.3 Mycobrowser_v5 CDS 1587772 1588482 . - 0 Rv1411c lprG Unknown Conserved lipoprotein LprG Rv1411c, (MTCY21B4.28c), len: 236 aa. lprG (alternate gene name: P27), conserved lipoprotein, similar to Mycobacterium tuberculosis hypothetical lipoproteins e.g. Rv1270c|MTCY50.12 (35.1% identity in 245 aa overlap); Rv1368, Rv2945c. Contains N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein lipid attachment site (PS00013). Note that the Rv1410c-Rv1411c operon seems transcribed from two promoters in Mycobacterium bovis BCG (see Bigi et al., 2000). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WK45 No cell wall and cell processes 3MH8,3MH9,3MHA P0A5I8 P9WK45 GO:0005886,GO:0009405 P9WK45 NC_000962.3 Mycobrowser_v5 CDS 1591671 1592153 . + 0 Rv1416 ribH Riboflavin synthase is a bifunctional enzyme complex involved in riboflavin synthesis. Riboflavin synthase catalyzes the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes the condensation of 5-amino-6-(1'-D)-ribityl- amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone- 4-phosphate yielding 6,7-dimethyl-8-lumazine. Probable riboflavin synthase beta chain RibH (6,7-dimethyl-8-ribityllumazine synthase) (DMRL synthase) (lumazine synthase) Rv1416, (MTCY21B4.34), len: 160 aa. Probable ribH, riboflavin synthase beta chain, similar to many e.g. RISB_ECOLI|P25540 Escherichia coli (156 aa), FASTA scores: opt: 330, E(): 1.8e-15, (44.1% identity in 145 aa overlap). Note alternative GTG start possible overlapping the stop codon of Rv1415|MTCY21B4.33. Belongs to the DMRL synthase family. N-terminus extended since first submission (previously 154 aa). P9WHE9 No intermediary metabolism and respiration 1W19,1W29,2C92,2C94,2C97,2C9B,2C9D,2VI5 P66034 P9WHE9 GO:0004746,GO:0009349,GO:0009231 2.5.1.9 P9WHE9 NC_000962.3 Mycobrowser_v5 CDS 1594042 1595982 . + 0 Rv1420 uvrC Involved in nucleotide excision repair. The ABC excision nuclease is a DNA repair enzyme that catalyzes the excision reaction of UV-damaged nucleotide segments producing oligomers having the modified base(S). Attaches to the UVRA-UVRB complex, displacing UVRA, and the damaged DNA strand is nicked on both sides of the damaged site Probable excinuclease ABC (subunit C - nuclease) UvrC Rv1420, (MTCY21B4.38), len: 646 aa. Probable uvrC, excinuclease ABC, subunit C; nuclease (see citations below), similar to many e.g. UVRC_PSEFL|P32966 Pseudomonas fluorescens (607 aa), fasta scores: opt: 738, E(): 8.4e-39, (36.6% identity in 629 aa overlap). Belongs to the UvrC family. P9WFC5 No information pathways P67426 P9WFC5 GO:0009432,GO:0005737,GO:0009381,GO:0009380,GO:0006289,GO:0003677 P9WFC5 NC_000962.3 Mycobrowser_v5 CDS 1597906 1598883 . + 0 Rv1423 whiA Involved in transcriptional mechanism Probable transcriptional regulatory protein WhiA Rv1423, (MTCY21B4.41-MTCY493.31c), len: 325 aa. Putative whiA, transcriptional regulator, probably equivalent to AL035591|SCC54.10 whiA protein from Streptomyces coelicolor (328 aa), FASTA scores: opt: 1505, E(): 0, (70.4% identity in 324 aa overlap). Also some similarity to O06975|YVCL hypothetical protein from Bacillus subtilis (316 aa), FASTA scores: E(): 1.8e-0 8, (25.7% identity in 304 aa overlap). P9WF45 No regulatory proteins P71692 P9WF45 GO:0043937,GO:0045449,GO:0006350,GO:0003677 P9WF45 NC_000962.3 Mycobrowser_v5 CDS 1612342 1612950 . - 0 Rv1435c Rv1435c Unknown Probable conserved proline, glycine, valine-rich secreted protein Rv1435c, (MTCY493.19), len: 202 aa. Probable conserved Pro-, Gly-, Val-rich secreted protein (see citation below) with a N-terminal signal sequence. Similar at C-terminus to AF017099|AF017099_1 Mycobacterium tuberculosis pGB1 (87 aa), FASTA scores: opt: 550, E(): 2.3e-17, (97.7% identity in 86 aa overlap). Shows some similarity to N-terminus of CPN_DROME|Q02910 calphotin. Drosophila melanogaster (865 aa), FASTA scores: opt: 266, E(): 2.5e-05, (37.2% identity in 191 aa overlap). Contains at least five 7 aa imperfect repeats. Also shows similarity to other Mycobacterium tuberculosis proteins e.g. MTCI237.20c (34.7% identity in 193 aa overlap), MTCI65.25c (36.9% identity in 160 aa overlap) and MTCI65.24c (34.2% identity in 196 aa overlap). O06823 No cell wall and cell processes O06823 NC_000962.3 Mycobrowser_v5 CDS 1613307 1614326 . + 0 Rv1436 gap Involved in second phase of glycolysis (first step) [catalytic activity: D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.] Probable glyceraldehyde 3-phosphate dehydrogenase Gap (GAPDH) Rv1436, (MTCY493.18c), len: 339 aa. Probable gap, Glyceraldehyde 3-phosphate dehydrogenase, highly similar to many e.g. G3P_MYCLE|P46713 Mycobacterium leprae (339 aa), FASTA scores: opt: 1933, E():0, (89.1% identity in 339 aa overlap). Contains PS00071 Glyceraldehyde 3-phosphate dehydrogenase active site. Belongs to the glyceraldehyde 3-phosphate dehydrogenase family. P9WN83 No intermediary metabolism and respiration P64178 P9WN83 GO:0005737,GO:0004365,GO:0006096,GO:0055114,GO:0051287 1.2.1.12 P9WN83 NC_000962.3 Mycobrowser_v5 CDS 2894893 2895960 . - 0 Rv2571c Rv2571c Unknown Probable transmembrane alanine and valine and leucine rich protein Rv2571c, (MTCY227.30), len: 355 aa. Probable transmembrane ala-, val-, leu-rich protein, showing some similarity with other membrane proteins e.g. Q99340|YFDA_CORGL hypothetical integral membrane protein from Corynebacterium glutamicum (Brevibacterium flavum) (359 aa), FASTA scores: opt: 338, E(): 2.5e-13, (29.4% identity in 255 aa overlap); Q9RD86|SCF43.02 putative integral membrane protein from Streptomyces coelicolor (379 aa), FASTA scores: opt: 208, E(): 2.1e-05, (26.05% identity in 303 aa overlap); Q9RD81|SCF43.07 putative integral membrane protein from Streptomyces coelicolor (419 aa), FASTA scores: opt: 205, E(): 3.5e-05, (25.15% identity in 362 aa overlap); etc. P9WL89 No cell wall and cell processes P65015 P9WL89 GO:0005886,GO:0016021 P9WL89 NC_000962.3 Mycobrowser_v5 CDS 1625418 1626962 . - 0 Rv1447c zwf2 Involved in pentose phosphate pathway [catalytic activity: D-glucose 6-phosphate + NADP(+) = D-glucono- 1,5-lactone 6-phosphate + NADPH] Probable glucose-6-phosphate 1-dehydrogenase Zwf2 (G6PD) Rv1447c, (MTCY493.07), len: 514 aa. Probable zwf2 (ZWF), Glucose-6-phosphate 1-dehydrogenase, highly similar to many e.g. G6PD_SYNY3|P73411 Synechocystis sp. (509 aa), FASTA scores: opt: 1578, E(): 0, (46.8% identity in 509 aa overlap). Also similar to M. tuberculosis Rv1121, zwf glucose-6-phosphate 1-dehydrogenase. Contains PS00069 Glucose-6-phosphate dehydrogenase active site. Mycobacterium tuberculosis has two genes for ZWF. This one looks like a classical ZWF. Belongs to the glucose-6-phosphate dehydrogenase family. P9WN73 No intermediary metabolism and respiration P0A584 P9WN73 GO:0006006,GO:0004345,GO:0055114 1.1.1.49 P9WN73 NC_000962.3 Mycobrowser_v5 CDS 1628097 1630199 . - 0 Rv1449c tkt This enzyme, together with transaldolase, provides a link between the glycolytic and pentose-phosphate pathways. It catalyzes the reversible transfer of a two-carbon KETOL unit from xylulose 5-phosphate to an aldose receptor [catalytic activity: sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate] Transketolase Tkt (TK) Rv1449c, (MTCY493.05), len: 700 aa. tkt, transketolase. Highly similar to several e.g. TKT_MYCLE|P46708 transketolase (tk) from Mycobacterium leprae (699 aa), FASTA scores: opt: 4216, E(): 0, (89.1% identity in 700 aa overlap). Start site chosen by homology. Contains PS00801 Transketolase signature 1. Belongs to the transketolase family. Thought to be differentially expressed within host cells (see Triccas et al., 1999). P9WG25 No intermediary metabolism and respiration O06811 P9WG25 GO:0046872,GO:0004802,GO:0008152 2.2.1.1 P9WG25 NC_000962.3 Mycobrowser_v5 CDS 1636004 1638229 . - 0 Rv1452c PE_PGRS28 Function unknown PE-PGRS family protein PE_PGRS28 Rv1452c, (MTCY493.02), len: 741 aa. PE_PGRS28, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan and Delogu, 2002), similar to Y03A_MYCTU|Q10637 hypothetical glycine-rich 49.6 kDa protein (603 aa), fasta scores: opt: 2090, E(): 0, (56.3% identity in 641 aa overlap). Q79FP1 No PE/PPE Q79FP1 Q79FP1 Q79FP1 NC_000962.3 Mycobrowser_v5 CDS 1644363 1646138 . - 0 Rv1459c Rv1459c Unknown Possible conserved integral membrane protein Rv1459c, (MTV007.06c), len: 591 aa. Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). Possible conserved integral membrane protein, equivalent to MLCL536.30|Z99125 hypothetical protein from Mycobacterium leprae (593 aa), FASTA scores: opt: 1670, E(): 0, (78.6% identity in 585 aa overlap). Also similar to M. tuberculosis protein Rv2174|MTV021.07 (33.1% identity in 523 aa overlap). O53150 No cell wall and cell processes O53150 NC_000962.3 Mycobrowser_v5 CDS 1646989 1649529 . + 0 Rv1461 Rv1461 Function unknown Conserved protein Rv1461, (MTV007.08), len: 846 aa. Conserved protein. Equivalent of spliced protein from Mycobacterium leprae MLCL536.28c len: 869. Residues 1-253 represent N-extein, and 613-846 the C-extein. The intein present from residues 254 - 612 is different in sequence and site of the insertion from the one present in MLCL536.28c. FASTA scores: Z99125|MLCL536_23 Mycobacterium leprae cosmid L536 (869 aa), opt: 1498 E(): 0, (54.1% identity in 917 aa overlap). The mature protein is similar to Z99120|BSUB0017_150 hypothetical Bacillus subtilis protein (465 aa), FASTA scores: opt:1053, E(): 0, (34.8% identity in 821 aa overlap). The intein shows some similarity to inteins from U67548|MJU67548_6 Methanococcus jannaschii (895 aa), FASTA scores: opt: 181, E(): 0.00023, (25.2% identity in 274 aa overlap). P9WFP7 No conserved hypotheticals P67125 P9WFP7 GO:0016539,GO:0006314,GO:0016226,GO:0005515,GO:0004519 3.1.-.- P9WFP7 NC_000962.3 Mycobrowser_v5 CDS 1651518 1652771 . + 0 Rv1464 csd Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L-selenocystine to produce L-alanine Probable cysteine desulfurase Csd Rv1464, (MTV007.11), len: 417 aa. Probable csd, cysteine desulfurase. Equivalent to Q49690|MLCL536.25C cysteine desulfurase from Mycobacterium leprae (418 aa), FASTA scores: opt: 2333, E(): 0, (85.4% identity in 417 aa overlap); and similar to cysteine desulfurase from other organisms. Also similar to M. tuberculosis proteins Rv3025c|ISCS and Rv3778c. Contains PS00595 Aminotransferases class-V pyridoxal-phosphate attachment site. Belongs to class-V of pyridoxal-phosphate-dependent aminotransferases. CSD subfamily. P9WQ69 No intermediary metabolism and respiration P63516 P9WQ69 GO:0006534,GO:0030170,GO:0031071 2.8.1.7 P9WQ69 NC_000962.3 Mycobrowser_v5 CDS 1653673 1655502 . - 0 Rv1467c fadE15 Function unknown, but involvement in lipid degradation. Probable acyl-CoA dehydrogenase FadE15 Rv1467c, (MTV007.14c), len: 609 aa. Probable fadE15, acyl-CoA dehydrogenase, highly similar to NP_302639.1|NC_002677 acyl-CoA dehydrogenase from Mycobacterium leprae (611 aa). Also highly similar to many e.g. T36481 probable acyl-CoA dehydrogenase (fragment) from Streptomyces coelicolor (491 aa) (has its N-terminus very shorter); NP_384640.1|NC_003047 putative acyl-CoA dehydrogenase protein from Sinorhizobium meliloti (598 aa); ACDS_MEGEL|Q06319 acyl-CoA dehydrogenase (short-chain specific) from Megasphaera elsdenii (383 aa), FASTA scores: E(): 2e-12, (25.4% identity in 410 aa overlap); etc. Also highly similar to fadE5|Rv0244c|MTV034.10c acyl-CoA dehydrogenase from Mycobacterium tuberculosis (611 aa); and similar to other proteins from Mycobacterium tuberculosis. O53158 No lipid metabolism O53158 O53158 GO:0003995,GO:0055114,GO:0050660 1.3.99.- O53158 NC_000962.3 Mycobrowser_v5 CDS 1679322 1680629 . + 0 Rv1490 Rv1490 Unknown Probable membrane protein Rv1490, (MTCY277.12), len: 435 aa. Probable membrane protein. P9WLX1 No cell wall and cell processes P9WLX1 GO:0005886,GO:0016021 P9WLX1 NC_000962.3 Mycobrowser_v5 CDS 1656963 1658936 . + 0 Rv1469 ctpD Cation-transporting ATPase; possibly catalyzes the transport of a cation (possibly cadmium) with the hydrolyse of ATP [catalytic activity: ATP + H(2)O + cation(in) = ADP + phosphate + cation(out)]. Probable cation transporter P-type ATPase D CtpD Rv1469, (MTV007.16), len: 657 aa. Probable ctpD, cation-transporting P-type ATPase D (transmembrane protein), highly similar to others e.g. T35947 probable cation-transporting ATPase from Streptomyces coelicolor (638 aa); NP_442633.1|NC_000911 cation-transporting ATPase (E1-E2 ATPase) from Synechocystis sp. strain PCC 6803 (642 aa), FASTA scores: opt: 1438, E(): 0, (41.9% identity in 592 aa overlap); NP_389268.1|NC_000964 protein similar to heavy metal-transporting ATPase from Bacillus subtilis (637 aa); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. Rv3743c|MTV025.091c|CTPJ (660 aa). Contains PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases), subfamily IB. P9WPT3 No cell wall and cell processes P63685 P9WPT3 GO:0006754,GO:0015662,GO:0005261,GO:0016021,GO:0046872,GO:0030001,GO:0005886,GO:0005524 3.6.3.- P9WPT3 NC_000962.3 Mycobrowser_v5 CDS 1659370 1659741 . + 0 Rv1471 trxB1 Thioredoxin participates in various redox reactions through the reversible oxidation of its active center dithiol, to a disulfide, & catalyzes dithiol-disulfide exchange reactions. Probable thioredoxin TrxB1 Rv1471, (MTV007.18), len: 123 aa. Probable trxB1, thioredoxin, similar to many bacterial thioredoxins e.g. P33636|THI2_ECOLI from Escherichia coli (139 aa), FASTA scores: opt: 290, E(): 1.8e-13, (44.3% identity in 97 aa overlap); etc. Highly similar to Rv1470|TrxA probable thioredoxin from Mycobacterium tuberculosis (124 aa), FASTA scores: opt: 402, E(): 1.2e-32, (54.4% identity in 114 aa overlap). Contains PS00194 Thioredoxin family active site. Belongs to the thioredoxin family. Note that previously known as trxB. L7N664 No intermediary metabolism and respiration Q7D8E1 L7N664 GO:0009055,GO:0006662,GO:0055114,GO:0015035,GO:0045454 1.-.-.- L7N664 NC_000962.3 Mycobrowser_v5 CDS 1659763 1660620 . + 0 Rv1472 echA12 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Possible enoyl-CoA hydratase EchA12 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv1472, (MTV007.19), len: 285 aa. Possible echA12, enoyl-CoA hydratase, highly similar to P53526|ECHH_MYCLE|NP_301896.1|NC_002677 possible enoyl-CoA hydratase/isomerase from Mycobacterium leprae (294 aa), FASTA scores: opt: 1265, E(): 0, (72.0% identity in 271 aa overlap). Also similar to others e.g. CAA66096.1|X97452 enoyl-CoA isomerase from Escherichia coli strain K12 (262 aa); CAC44593.1|AL596162 putative enoyl-CoA hydratase from Streptomyces coelicolor (275 aa); etc. Also similar to others from Mycobacterium tuberculosis e.g. ECHA16|Rv2831|MTCY16B7.11c (249 aa), FASTA scores: opt: 232, E(): 1.3e-15, (33.8% identity in 204 aa overlap); etc. P9WNN7 No lipid metabolism O53163 P9WNN7 GO:0006631,GO:0004300 4.2.1.17 P9WNN7 NC_000962.3 Mycobrowser_v5 CDS 1662641 1663204 . - 0 Rv1474c Rv1474c Involved in transcriptional mechanism Probable transcriptional regulatory protein Rv1474c, (MTV007.21c), len: 187 aa. Probable transcription regulator, equivalent to AF0021|AF002133_1 transcriptional regulator from Mycobacterium avium strain GIR10 (82 aa), FASTA scores: opt: 490, E(): 6.7e-26, (92.5% identity in 80 aa overlap). Also similar to Q59431|UIDR_ECOLI UID operon repressor (GUS operon) from Escherichia coli (196 aa), FASTA scores: opt: 192, E(): 5.8e-06, (28.5% identity in 172 aa overlap). Belongs to the TetR/AcrR family of transcriptional regulators. Helix turn helix motif predicted at aa 33-54 (+3.40 SD). O53165 No regulatory proteins O53165 O53165 GO:0006350,GO:0003700,GO:0006355 O53165 NC_000962.3 Mycobrowser_v5 CDS 1666990 1668408 . + 0 Rv1477 ripA Unknown, but supposed involvement in virulence. Peptidoglycan hydrolase Rv1477, (MTV007.24), len: 472 aa. RipA, peptidoglycan hydrolase (see Hett et al., 2007). Secreted, cell-associated protein. The last 277 residues are nearly identical to those of AF0060|AF006054_1 hypothetical invasion protein INV1 from Mycobacterium tuberculosis (277 aa), FASTA scores: opt: 1833, E(): 0, (98.2% identity in 277 aa overlap); also very similar to AF0021|AF002133_4 invasin 1 protein from Mycobacterium avium (273 aa), FASTA scores: opt: 1452, E(): 0, (78.1% identity in 279 aa overlap). Similar to Rv1566c|MTCY336.37|Z95586 Mycobacterium tuberculosis cosmid (230 aa), FASTA scores: opt: 528, E(): 4.4e-20, (52.0% identity in 150 aa overlap); and weakly similar to p60 proteins of Listeria spp throughout its length e.g. M80351|LISIAPB_1 Listeria monocytogenes iap-related protein (478 aa), FASTA scores: opt: 251, E(): 8e-06, (24.4% identity in 487 aa overlap). C-terminal domain highly similar to next orf Rv1478|MTV007.25. Interacts with RpfB and RpfE (see Hett et al., 2007). Predicted to be an outer membrane protein (See Song et al., 2008). O53168 No virulence, detoxification, adaptation 3PBC,3S0Q,2XIV,3NE0 O53168 O53168 O53168 NC_000962.3 Mycobrowser_v5 CDS 1678552 1678908 . + 0 Rv1489 Rv1489 Function unknown Conserved protein Rv1489, len: 118 aa. Conserved protein, similar to hypothetical proteins from Mycobacterium avium subsp. paratuberculosis and Streptomyces coelicolor e.g. AJ250017_1 insertion sequence IS900, Locus 3, putative invasion protein from M. paratuberculosis (138 aa), FASTA scores: opt: 120, E(): 0.26, (34.375% identity in 96 aa overlap); SCD6.11c|AL353815_11 possible integral membrane protein from Streptomyces coelicolor (136 aa), FASTA scores: opt: 106, E(): 2.2, (35.9% identity in 103 aa overlap). ORF predicted by GC plot. Replaces previous Rv1489c on other strand. L7N692 No conserved hypotheticals L7N692 NC_000962.3 Mycobrowser_v5 CDS 1672457 1673299 . - 0 Rv1482c Rv1482c Function unknown Conserved hypothetical protein Rv1482c, (MTCY277.03c), len: 280 aa. Conserved hypothetical protein, highly similar to O07396|AF002133 Mycobacterium avium protein MAV346 (346 aa), FASTA scores: E(): 0, (65.2% identity in 342 aa overlap); slight similarity to GRPE_ECOLI|P09372 heat shock protein from E. coli (197 aa), FASTA scores: opt: 139, E(): 0.012, (28.3% identity in 159 aa overlap). Similar to Mycobacterium tuberculosis hypothetical proteins Rv3517, Rv3555c, Rv3714c, Rv1073, etc. Start changed since first submission (-59 aa). P71763 No conserved hypotheticals P71763 P71763 NC_000962.3 Mycobrowser_v5 CDS 1674202 1675011 . + 0 Rv1484 inhA This isozyme is involved in mycolic acid biosynthesis. Second reductive step in fatty acid biosynthesis. Involved in the resistance against the antituberculosis drugs isoniazid and ethionamide [catalytic activity: acyl-[acyl-carrier protein] + NAD(+) = trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH]. NADH-dependent enoyl-[acyl-carrier-protein] reductase InhA (NADH-dependent enoyl-ACP reductase) Rv1484, (MTCY277.05), len: 269 aa. InhA, NADH-dependent enoyl-[acyl-carrier-protein] reductase (see citations below). Identical to INHA_MYCTU|P46533 enoyl-[acyl-carrier-protein] reductase from Mycobacterium tuberculosis and G1155270 Mycobacterium bovis enoyl acp reductase. Some similarity to the short-chain dehydrogenases/reductases (SDR) family. P9WGR1 No lipid metabolism 1BVR,1ENY,1ENZ,1P44,1P45,1ZID,2AQ8,2AQH,2AQI,2AQK,2B35,2B36,2B37,2H7I,2H7L,2H7M,2H7N,2H7P,2H9I,2IDZ,2IE0,2IEB,2IED,2NSD,2NTJ,2NV6,2PR2,2X22,2X23,3FNE,3FNF,3FNG,3FNH,3OEW,3OEY,3OF2 P9WGR1 GO:0004318,GO:0006633,GO:0055114,GO:0046677 1.3.1.9 ETH,INH P9WGR1 NC_000962.3 Mycobrowser_v5 CDS 1677397 1678542 . + 0 Rv1488 Rv1488 Function unknown Possible exported conserved protein Rv1488, (MTCY277.09), len: 381 aa. Possible exported conserved protein; contains possible N-terminal signal sequence. Similar to YBBK_ECOLI|P77367 hypothetical protein ybbK from Escherichia coli (305 aa), FASTA scores: opt: 716, E(): 0, (37.1% identity in 307 aa overlap). Similar to stomatin-like proteins e.g. AF065260|AF065260_1 Clostridium difficile (320 aa), FASTA scores: opt: 767, E(): 0, (42.3% identity in 307 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). P9WPR9 No cell wall and cell processes P63693 P9WPR9 GO:0016021 P9WPR9 NC_000962.3 Mycobrowser_v5 CDS 1681208 1681966 . - 0 Rv1491c Rv1491c Unknown Conserved membrane protein Rv1491c, (MTCY277.13c), len: 252 aa. Conserved membrane protein. Similar to hypothetical proteins from many organisms e.g. YDJZ_ECOLI|P76221 Escherichia coli (235 aa), FASTA scores: opt: 223, E():6.7 e-07, (31.7% identity in 145 aa overlap); AL133252|SCE46.15 Streptomyces coelicolor (249 aa), FASTA scores: opt: 378, E(): 1.5e-17, (39.1% identity in 169 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical protein Rv0625c. P9WFS3 No cell wall and cell processes P67117 P9WFS3 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1682157 1684004 . + 0 Rv1492 mutA Involved in propionic acid fermentation. Catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates [catalytic activity: (R)-2-methyl-3-oxopropanoyl-CoA = succinyl- CoA] Probable methylmalonyl-CoA mutase small subunit MutA (MCM) Rv1492, (MTCY277.14), len: 615 aa. Probable mutA, Methylmalonyl-CoA mutase small-subunit, strong similarity to e.g. MUTA_STRCM|Q05064 methylmalonyl-CoA mutase beta-subunit from Streptomyces cinnamonensis (616 aa), FASTA scores: opt: 1512, E(): 0, (45.9% identity in 628 aa overlap). Contains PS00213 Lipocalin signature, PS00544 Methylmalonyl-CoA mutase signature. Belongs to the methylmalonyl-CoA mutase family. P9WJK7 No lipid metabolism P65485 P9WJK7 GO:0019652,GO:0004494,GO:0031419 5.4.99.2 P9WJK7 NC_000962.3 Mycobrowser_v5 CDS 1690407 1690805 . + 0 Rv1499 Rv1499 Unknown Hypothetical protein Rv1499, (MTCY277.21), len: 132 aa. Hypothetical unknown protein; was initially longer but has been shortened (-24 aa) owing to overlap with Rv1498A. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P71780 No conserved hypotheticals P71780 NC_000962.3 Mycobrowser_v5 CDS 1690850 1691878 . + 0 Rv1500 Rv1500 Function unknown Probable glycosyltransferase Rv1500, (MTCY277.22), len: 342 aa. Probable glycosyltransferase, hydrophobic domain near C-terminus. Some similarity to putative glycosyl-transferases from Bacillus subtilis e.g. O34319|YKCC_BACSU (323 aa), opt: 490, E(): 6.1e-25, (28.85% identity in 312 aa overlap) and to N-acetyl glucosamine transferases. Also similar to G1001347 hypothetical 36.7 kDa protein (318 aa), FASTA scores: opt: 523, E(): 7.2e-26, (30.6% identity in 307 aa overlap). P9WMX5 No intermediary metabolism and respiration P71781 P9WMX5 GO:0005886,GO:0016757,GO:0016021 2.4.-.- P9WMX5 NC_000962.3 Mycobrowser_v5 CDS 1703074 1704096 . + 0 Rv1511 gmdA Unknown, probably involved in nucleotide-sugar metabolism GDP-D-mannose dehydratase GmdA (GDP-mannose 4,6 dehydratase) (GMD) Rv1511, (MTCY277.33), len: 340 aa. Probable gmdA, GDP-D-mannose dehydratase, equivalent to AF125999|AF125999_13 Mycobacterium avium enzyme (343 aa), FASTA scores: opt: 2085, E(): 0, (89.1% identity in 338 aa overlap); similar to G755218 pseudomonas aeruginosa GDP-D-mannose dehydratase (GCA) (323 aa), FASTA scores: opt: 1073, E(): 0, (51.9% identity in 320 aa overlap); and to S74433 GDP-D-mannose dehydratase rfbD - Syn (362 aa), FASTA scores: opt: 1405, E(): 0, (63.9% identity in 327 aa overlap). P71790 No intermediary metabolism and respiration P71790 P71790 GO:0019673,GO:0050662,GO:0005622,GO:0008446 4.2.1.47 P71790 NC_000962.3 Mycobrowser_v5 CDS 1704093 1705061 . + 0 Rv1512 epiA Unknown, probably involved in nucleotide-sugar metabolism Probable nucleotide-sugar epimerase EpiA Rv1512, (MTCY277.34), len: 322 aa. Probable epiA, nucleotide sugar epimerase, equivalent to AJ223832|MAS223832_4 from Mycobacterium avium silvaticum (339 aa), FASTA scores: opt: 1821, E(): 0, (84.6% identity in 318 aa overlap); and similar to WCAG_ECOLI|P32055 colanic acid biosynthesis protein wcaG (321 aa), FASTA scores: opt: 835, E(): 0, (53.5% identity in 316 aa overlap). P71791 No intermediary metabolism and respiration P71791 P71791 GO:0044237,GO:0050662,GO:0003824 P71791 NC_000962.3 Mycobrowser_v5 CDS 1712302 1714053 . + 0 Rv1521 fadD25 Function unknown, but involvement in lipid degradation. Probable fatty-acid-AMP ligase FadD25 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) Rv1521, (MTCY19G5.07), len: 583 aa. Probable fadD25, fatty-acid-AMP synthetase, highly similar to many e.g. P71495|U75685 acyl-CoA synthase from Mycobacterium bovis (582 aa), FASTA scores: opt: 2486, E(): 0, (63.4% identity in 584 aa overlap); NP_301232.1|NC_002677 acyl-CoA synthetase from Mycobacterium leprae (579 aa); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. fadD24 (584 aa); fadD28 (580 aa); etc. P9WQ45 No lipid metabolism Q50586 P9WQ45 GO:0016021,GO:0016874,GO:0005886,GO:0006631 6.2.1.- P9WQ45 NC_000962.3 Mycobrowser_v5 CDS 1714172 1717612 . - 0 Rv1522c mmpL12 Unknown. Thought to be involved in fatty acid transport. Probable conserved transmembrane transport protein MmpL12 Rv1522c, (MTCY19G5.06), len: 1146 aa. Probable mmpL12, conserved transmembrane transport protein (see Tekaia et al., 1999), member of RND superfamily. Strong similarity to many Mycobacterial membrane proteins e.g. Q49619|G466786 putative transport protein B1170_C1_181 from Mycobacterium leprae (1008 aa), FASTA scores: opt: 2418, E(): 0, (51.0% identity in 1006 aa overlap); etc. Also highly similar to MmpL8|MTCY48.08c|Rv3823c probable conserved transmembrane transport protein from Mycobacterium tuberculosis, FASTA score: (34.3% identity in 376 aa overlap); and some similarity to MmpL10|MTCY20G9|Rv1183 probable conserved transmembrane transport protein, FASTA score: (27.2% identity in 1011 aa overlap). Belongs to the MmpL family. P9WJT7 No cell wall and cell processes Q50585 P9WJT7 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1720780 1722060 . - 0 Rv1526c Rv1526c Unknown; thought to be involved in cellular metabolism. Probable glycosyltransferase Rv1526c, (MTCY19G5.02), len: 426 aa. Probable glycosyltransferase, highly similar to G467196 Protein L518_C2_147 from Mycobacterium leprae (421 aa), FASTA scores, opt: 1497, E(): 0, (55.0% identity in 424 aa overlap); similar to G452504 rhamnosyltransferase (24.7% identity in 433 aa overlap); and P96565|U84350 glycosyltransferase GTFE from Amycolatopsis orientalis (408 aa), E(): 3.4e-24, (28.4% identity in 429 aa overlap), also high similarity to Rv1524|MTCY19G5.04c (58.7 % identity in 416 aa overlap). P9WLV1 No intermediary metabolism and respiration P64869 P9WLV1 GO:0030259,GO:0016758,GO:0005975 P9WLV1 NC_000962.3 Mycobrowser_v5 CDS 1753716 1755431 . + 0 Rv1550 fadD11 Function unknown, but involvement in lipid degradation. Probable fatty-acid-CoA ligase FadD11 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv1550, (MTCY48.15c), len: 571 aa. Probable fadD11, fatty-acid-CoA synthetase, similar, except in N-terminus, to many e.g. SC6A5.39|T35430 probable long-chain-fatty-acid--CoA ligase from Streptomyces coelicolor (612 aa); NP_301672.1|NC_002677 putative long-chain-fatty-acid-CoA ligase from Mycobacterium leprae (600 aa); P44446|LCFH_HAEIN putative long-chain-fatty-acid-CoA ligase from Haemophilus influenzae (607 aa), FASTA scores: opt: 762, E(): 2.3e-38, (34.4% identity in 436 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family. Possible frameshift with respect to previous ORF Rv1549|MTCY48.16c but we can find no sequence error to account for this. P9WQ53 No lipid metabolism Q10776 P9WQ53 GO:0016021,GO:0016874,GO:0005886,GO:0006631 6.2.1.- P9WQ53 NC_000962.3 Mycobrowser_v5 CDS 1739856 1741247 . + 0 Rv1537 dinX Involved in DNA metabolism [catalytic activity: N deoxynucleoside triphosphate = N diphosphate + {DNA}N]. Probable DNA polymerase IV DinX (pol IV 1) (DNA nucleotidyltransferase (DNA-directed)) Rv1537, (MTCY48.28c, MT1589), len: 463 aa. Probable dinX (alternate gene name: dinB1), DNA polymerase IV. Similar to umuC, mucB, samb, and impb (UV protection and mutation) e.g. IMPB_SALTY|P18642 impb protein from Salmonella typhimurium (424 aa), FASTA scores: opt: 386, E(): 1.7e-17, (27.5% identity in 415 aa overlap); etc. Also similar to Mycobacterium tuberculosis Rv3056|dinP. Belongs to the DNA polymerase type-Y family. P9WNT3 No information pathways P63985 P9WNT3 GO:0006261,GO:0003887,GO:0005737,GO:0003684,GO:0000287,GO:0006281 2.7.7.7 P9WNT3 NC_000962.3 Mycobrowser_v5 CDS 1751297 1753333 . - 0 Rv1548c PPE21 Function unknown PPE family protein PPE21 Rv1548c, (MTCY48.17), len: 678 aa. PPE21, Member of the Mycobacterium tuberculosis PPE family, similar to several e.g. YHS6_MYCTU|P42611 hypothetical 50.6 kDa protein in hsp65 3' region (517 aa), FASTA scores: opt:1142, E(): 0, (40.6% identity in 616 aa overlap); also similar to MTCY31.06c (54.9% identity in 381 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). P9WI21 No PE/PPE Q10778 P9WI21 GO:0005886,GO:0016021 P9WI21 NC_000962.3 Mycobrowser_v5 CDS 1757681 1759432 . + 0 Rv1552 frdA Involved in interconversion of fumarate and succinate (anaerobic respiration) [catalytic activity: succinate + acceptor = fumarate + reduced acceptor]. Probable fumarate reductase [flavoprotein subunit] FrdA (fumarate dehydrogenase) (fumaric hydrogenase) Rv1552, (MTCY48.13c), len: 583 aa. Probable frdA, fumarate reductase, flavoprotein subunit, highly similar to others e.g. P00363|FRDA_ECOLI fumarate reductase flavoprotein subunit from Escherichia coli strain K12 (601 aa), FASTA scores: opt: 2102, E(): 0, (54.7% identity in 585 aa overlap); NP_232284.1|NC_002505 fumarate reductase, flavoprotein subunit from Vibrio cholerae (602 aa); frdA|NP_438995.1|NC_000907 fumarate reductase, flavoprotein subunit from Haemophilus influenzae (599 aa); etc. Contains PS00504 Fumarate reductase / succinate dehydrogenase FAD-binding site. Note that fumarate reductase forms part of an enzyme complex containing four subunits: a flavoprotein (Rv1552|frdA), an iron-sulfur (Rv1553|frdB), and two hydrophobic anchor proteins (Rv1554|frdC and Rv1555|frdD). P9WN91 No intermediary metabolism and respiration P64174 P9WN91 GO:0009061,GO:0009055,GO:0022900,GO:0000104,GO:0006810,GO:0050660 1.3.99.1 P9WN91 NC_000962.3 Mycobrowser_v5 CDS 1761744 1762937 . + 0 Rv1557 mmpL6 Unknown. Thought to be involved in fatty acid transport. Probable conserved transmembrane transport protein MmpL6 Rv1557, (MTCY48.08c), len: 397 aa. Probable mmpL6, conserved transmembrane transport protein (see citations below). Member of RND superfamily, with strong similarity to C-terminal part of members of large Mycobacterial membrane protein family belonging to RND superfamily including: mmpL1, mmpL2, mmpL3, etc. Probably truncated (see Brosch et al., 2002). Belongs to the MmpL family. P9WJU9 No cell wall and cell processes Q10773 P9WJU9 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 1765400 1767142 . - 0 Rv1562c treZ Involved in trehalose biosynthesis (protective effect). Mycobacteria can produce trehalose from glucose 6-phosphate and UDP-glucose (the OTSA-OTSB pathway) from glycogen-like alpha(1-->4)-linked glucose polymers (the trey-TREZ pathway) and from maltose (the TRES pathway). Seems to have additional alpha-glucosidase activity. Maltooligosyltrehalose trehalohydrolase TreZ Rv1562c, (MTCY48.03), len: 580 aa. TreZ (previously called glgZ), Maltooligosyltrehalose trehalohydrolase, confirmed biochemically (see citation below). Similar to Q44316|D63343 TREZ maltooligosyl trehalose trehalohydrolase from arthrobacter SP (598 aa), FASTA scores: opt: 2071, E(): 0, (52.2% identity in 582 aa overlap); also similar to 1,4-alpha-glucan branching enzymes e.g. GLGB_BACST|P30538 (639 aa), FASTA scores: opt: 313, E(): 3.8e-13, (27.5% identity in 462 aa overlap). Also similar to Mycobacterium tuberculosis proteins Rv1326c|glgB, and Rv1563c treY (previously glgY). P9WQ23 No virulence, detoxification, adaptation Q10769 P9WQ23 GO:0043169,GO:0005737,GO:0005992,GO:0033942 3.2.1.141 P9WQ23 NC_000962.3 Mycobrowser_v5 CDS 1769436 1771601 . - 0 Rv1564c treX Possibly involved in trehalose biosynthesis (protective effect). Mycobacteria can produce trehalose from glucose 6-phosphate and UDP-glucose (the OtsA-OtsB pathway) from glycogen-like alpha(1-->4)-linked glucose polymers (the TreY-TreZ pathway) and from maltose (the TreS pathway). Probable maltooligosyltrehalose synthase TreX Rv1564c, (MTCY48.01), len: 721 aa. Probable treX (previously called glgX), Maltooligosyltrehalose synthase. Strong similarity to D83245|g1890053 treX, glycogen debranching enzyme (glgX) from Sulfolobus acidocaldarius (713 aa), FASTA score: opt: 2396, E(): 0, (48.4% identity in 709 aa overlap); similar to GLGX_HAEIN|P45178 glycogen operon protein glgx (659 aa), FASTA scores: opt: 1512, E(): 0, (42.3% identity in 645 aa overlap). P9WQ25 No virulence, detoxification, adaptation P0A4Y4 P9WQ25 GO:0005980,GO:0004133,GO:0004553,GO:0043169 3.2.1.- P9WQ25 NC_000962.3 Mycobrowser_v5 CDS 1771640 1773829 . - 0 Rv1565c Rv1565c Unknown Conserved hypothetical membrane protein Rv1565c, (MTCY336.38), len: 729 aa. Conserved hypothetical membrane protein, some similarity to O05402 hypothetical 72.2 kDa protein from Bacillus subtilis (634 aa), FASTA results: opt: 384, E(): 4.8e-17, (29.1% identity in 378 aa overlap); and to Y392_HAEIN|P43993 hypothetical protein hi0392 from H. influenzae (245 aa), FASTA results: opt: 265, E(): 5.5e-10, (28.3% identity in 247 aa overlap). C-terminal half equivalent to AL049478|MLCL458_19 (274 aa) (78.5% identity in 274 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins Rv0111, Rv0228, Rv1254, Rv0517. N-terminal half hydrophobic. O06625 No cell wall and cell processes O06625 O06625 GO:0016747,GO:0016020 NC_000962.3 Mycobrowser_v5 CDS 1775392 1776705 . + 0 Rv1568 bioA Involved in bioconversion of pimelate into dethiobiotin [catalytic activity: S-adenosyl-L-methionine + 8-amino-7- oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8- diaminononanoate]. Supposedly involved in stationary-phase survival. Adenosylmethionine-8-amino-7-oxononanoate aminotransferase BioA Rv1568, (MTCY336.35c), len: 437 aa. bioA, adenosylmethionine-8-amino-7-oxononanoate aminotransferase , equivalent to a predicted homologous protein from Mycobacterium smegmatis (see citation below). Highly similar to BIOA_MYCLE|P4548 from Mycobacterium leprae (436 aa), FASTA results: opt: 2534, E(): 0, (85.1% identity in 436 aa overlap). Also similar to other Mycobacterium tuberculosis proteins e.g. MTCY227.12c (449 aa), FASTA score: E(): 3.5e-16, (29.5% identity in 421 aa overlap). Contains aminotransferases class-III pyridoxal-phosphate attachment site (PS00600). Belongs to class-III of pyridoxal-phosphate-dependent aminotransferases. P9WQ81 No intermediary metabolism and respiration 3BV0,3LV2,3TFT,3TFU P0A4X6 P9WQ81 GO:0009102,GO:0005737,GO:0030170,GO:0004015 2.6.1.62 P9WQ81 NC_000962.3 Mycobrowser_v5 CDS 1780643 1782064 . - 0 Rv1576c Rv1576c Unknown Probable PhiRv1 phage protein Rv1576c, (MTCY336.28), len: 473 aa. Probable phiRV1 phage protein (capsid subunit) (see citation below). Highly similar to hypothetical Mycobacterium tuberculosis protein Rv2650c|MTCY441.19 phiRV2 phage related protein, FASTA scores: opt: 2782, E(): 0, (89.1% identity in 468 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06614 No insertion seqs and phages O06614 NC_000962.3 Mycobrowser_v5 CDS 1784497 1785912 . - 0 Rv1582c Rv1582c Unknown Probable PhiRv1 phage protein Rv1582c, (MTCY336.22), len: 471 aa. Probable phiRv1 phage protein (see citation below). N-terminus is similar to C-terminus of Q38030 ORF9 Bacteriophage phi-C31 (519 aa), FASTA scores: opt: 331, E(): 6.5e-15, (28.5% identity in 235 aa overlap); and C-terminus to whole of Q38031 ORF10 of Bacteriophage phi-C31 (202 aa), FASTA scores: opt: 353, E(): 1e-16, (31.1% identity in 190 aa overlap). Also similar to part of AB016282|AB016282_42 Bacteriophage phi-105 (806 aa), FASTA scores: opt: 790, E(): 0, (32.7% identity in 459 aa overlap). Similarity to other phage proteins described as putative DNA-polymerase or DNA-primase. Also slightly similar to MTCY441.24c, FASTA scores: E(): 0.0055, (36.0% identity in 75 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06608 No insertion seqs and phages O06608 O06608 O06608 NC_000962.3 Mycobrowser_v5 CDS 1787096 1788505 . - 0 Rv1586c Rv1586c Integration of phiRv1 into chromosome. Probable PhiRv1 integrase Rv1586c, (MTCY336.18), len: 469 aa. Probable phiRv1 integrase, possibly member of the serine family of recombinases (see citation below), similar to several bacteriophage integrases e.g. Q37839 ORF469 protein from Bacteriophage R4 (469 aa), FASTA scores: opt: 623, E(): 1.6e-29, (31.1% identity in 482 aa overlap); and Bacteriophage TP901-1. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O06604 No insertion seqs and phages O06604 O06604 GO:0006310,GO:0000150,GO:0003677 O06604 NC_000962.3 Mycobrowser_v5 CDS 1802035 1802667 . + 0 Rv1601 hisB Histidine biosynthesis (seventh step) [catalytic activity: D-erythro-1-(imidazol-4-YL)glycerol 3- phosphate = 3-(imidazol-4-YL)-2-oxopropyl phosphate + H(2)O] Probable imidazole glycerol-phosphate dehydratase HisB Rv1601, (MTCY336.03c), len: 210 aa. Probable hisB, imidazole glycerol-phosphate dehydratase. Similar to many e.g. HIS7_STRCO|P16247 from Streptomyces coelicolor (197 aa),FASTA results: opt: 763, E(): 0, (57.4% identity in 202 aa overlap). Belongs to the imidazoleglycerol-phosphate dehydratase family. P9WML9 No intermediary metabolism and respiration P64368 P9WML9 GO:0000105,GO:0004424,GO:0005737 4.2.1.19 P9WML9 NC_000962.3 Mycobrowser_v5 CDS 1793997 1794707 . - 0 Rv1593c Rv1593c Function unknown Conserved protein Rv1593c, (MTCY336.11), len: 236 aa. Conserved protein, highly similar to Q49628|B1170_F1_44 from Mycobacterium leprae (286 aa), FASTA scores: opt: 1304, E (): 0, (85.4% identity in 233 aa overlap); similar to several putative DNA hydrolases e.g. Q9S233|SCI51.07C from Streptomyces coelicolor (239 aa), FASTA scores: opt: 415, E(): 4.6e-20, (34.8% identity in 221 aa overlap); also similar to P74291|SLR1690 hypothetical protein from synechocystis (261 aa), FASTA scores: opt: 228, E(): 1.4e-17, (31.5% identity in 213 aa overlap). O06597 No conserved hypotheticals O06597 O06597 GO:0016787 O06597 NC_000962.3 Mycobrowser_v5 CDS 1795805 1797388 . + 0 Rv1595 nadB Quinolinate biosynthesis. Catalyzes the oxidation of L-aspartate to iminoaspartate which is condensed with dihydroxyacetone phosphate to quinolinate under the action of quinolinate synthase a [catalytic activity: L-aspartate + H(2)O + O(2) = oxaloacetate + NH(3) + H(2)O(2)] Probable L-aspartate oxidase NadB Rv1595, (MTCY336.09c), len: 527 aa. Probable nadB, L-aspartate oxidase. Similar to many e.g. Q49617 L-aspartate oxidase (quinolinate synthetase) from Mycobacterium leprae (424 aa), FASTA results: opt: 2152, E(): 0, (82.0% identity in 400 aa overlap). Also shows some similarity to Rv1552 frdA from Mycobacterium tuberculosis (583 aa), FASTA results: E(): 1e-10, (35.3% identity in 566 aa overlap). Heterodimer. The quinolinate synthetase complex consists of the two enzymes quinolinate synthetase a and B. P9WJJ9 No intermediary metabolism and respiration P65499 P9WJJ9 GO:0009435,GO:0005737,GO:0009055,GO:0055114,GO:0008734 1.4.3.16 P9WJJ9 NC_000962.3 Mycobrowser_v5 CDS 1947861 1949345 . + 0 Rv1722 Rv1722 Function unknown, but supposed involvement in lipid metabolism Possible carboxylase Rv1722, (MTCY04C12.07), len: 494 aa. Possible carboxylases. Weak similarity to several e.g. ACCC_BACSU|P49787 biotin carboxylase from Bacillus subtilis (448 aa), fasta scores: opt: 171, E(): 0.00021, (22.8% identity in 237 aa overlap). P71980 No lipid metabolism P71980 GO:0005524,GO:0003824 P71980 NC_000962.3 Mycobrowser_v5 CDS 1800896 1802038 . + 0 Rv1600 hisC1 Histidine biosynthesis (eighth step) [catalytic activity: L-histidinol-phosphate + 2-oxoglutarate = 3-(imidazol-4-YL)-2-oxopropyl phosphate + L-glutamate] Probable histidinol-phosphate aminotransferase HisC1 Rv1600, (MTCY336.04c), len: 380 aa. Probable hisC1, histidinol-phosphate aminotransferase O06591. Similar to many e.g. HIS8_STRCO|P16246 from Streptomyces coelicolor (369 aa), FASTA results: opt: 1353, E(): 0, (59.0% identity in 356 aa overlap). Some similarity to other Mycobacterium tuberculosis aminotransferases e.g. Rv3772|MTCY13D12.06, FASTA results: E(): 7.4e-25, (33.7% identity in 365 aa overlap). Contains aminotransferases class-II pyridoxal-phosphate attachment site (PS00599). Belongs to class-II of pyridoxal-phosphate-dependent aminotransferases. Note that previously known as hisC. P9WML7 No intermediary metabolism and respiration P0A678 P9WML7 GO:0004400,GO:0030170,GO:0000105 2.6.1.9 P9WML7 NC_000962.3 Mycobrowser_v5 CDS 1892270 1893562 . - 0 Rv1666c cyp139 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Probable cytochrome P450 139 Cyp139 Rv1666c, (MT1706, MTV047.02c), len: 430 aa. Probable cyp139, cytochrome P450, similar to many e.g. U38537|APU38537_7 from Anabaena sp. (459 aa), FASTA scores: opt: 516, E(): 1.7e-26, (25.8% identity in 418 aa overlap). Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. Belongs to the cytochrome P450 family. P9WPM1 No intermediary metabolism and respiration P63719 P9WPM1 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPM1 NC_000962.3 Mycobrowser_v5 CDS 1805653 1806000 . + 0 Rv1606 hisI Involved in histidine biosynthesis pathway (at the third step) [catalytic activity: 5-phosphoribosyl-AMP + H(2)O = 5-(5-phospho-D-ribosylaminoformimino)-1-(5-phospho-ribosyl) imidazole-4- carboxamide.] Probable phosphoribosyl-AMP 1,6 cyclohydrolase HisI Rv1606, (MTV046.04), len: 115 aa. Probable hisI, phosphoribosyl-AMP 1,6 cyclohydrolase, similar to several e.g. X82010|RSHISI_2 HISI from Rhodobacter sphaeroides (119 aa), FASTA scores: opt: 378, E(): 2.8e-21, (52.3% identity in 109 aa overlap); etc. P9WMM7 No intermediary metabolism and respiration P0A5B3 P9WMM7 GO:0000105,GO:0004635,GO:0005737 3.5.4.19 P9WMM7 NC_000962.3 Mycobrowser_v5 CDS 1806181 1807263 . + 0 Rv1607 chaA Involved in transport of ions (presumably calcium) across the membrane. Responsible for the translocation of the substrate across the membrane. Probable ionic transporter integral membrane protein ChaA Rv1607, (MTV046.05), len: 360 aa. Probable chaA, ionic transporter integral membrane protein, putative calcium/proton antiporter, similar to many e.g. P31801|CHAA_ECOLI calcium/proton antiporter from Escherichia coli (366 aa), FASTA scores: opt: 736, E(): 0, (35.9% identity in 351 aa overlap). Equivalent to Mycobacterium leprae AL049913|MLCB1610_21 (77.7% identity in 364 aa overlap). Seems to belong to the CaCA family. O53910 No cell wall and cell processes O53910 O53910 GO:0055085,GO:0016021 O53910 NC_000962.3 Mycobrowser_v5 CDS 1807903 1809453 . + 0 Rv1609 trpE Involved in tryptophan biosynthesis pathway (at the first step). Supposed tetramer of two components I and two components II: component I (Rv1609|trpE) catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II (Rv0013|trpG) provides glutamine amidotransferase activity [catalytic activity: chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate]. Anthranilate synthase component I TrpE (glutamine amidotransferase) Rv1609, (MTCY01B2.01, MTV046.07), len: 516 aa. trpE, anthranilate synthase component I. FASTA best: TRPE_CLOTM|P14953 anthranilate synthase component I from Clostridium thermocellum (494 aa), E(): 0, (42.6% identity in 498 aa overlap). Some similarity to Rv2386c|MTCY253.35, E(): 6.3e-17; and Rv3215|MTCY07D11.11c, E(): 5.7e-15. Belongs to the anthranilate synthase component I family. P9WFX3 No intermediary metabolism and respiration P67001 P9WFX3 GO:0000162,GO:0004049 4.1.3.27 P9WFX3 NC_000962.3 Mycobrowser_v5 CDS 1811127 1812359 . + 0 Rv1612 trpB Tryptophan biosynthesis pathway (fifth last step). The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine. [catalytic activity: L-serine + 1-(indol-3-YL)glycerol 3-phosphate = L-tryptophan + glyceraldehyde 3-phosphate + H(2)O] Tryptophan synthase, beta subunit TrpB Rv1612, (MTCY01B2.04), len: 410 aa. TrpB, tryptophan synthase beta chain. Equivalent to AL049913|MLCB1610_25 from Mycobacterium leprae (340 aa) (88.5% identity in 331 aa overlap). Similar to others e.g. TRPB_CAUCR|P12290 tryptophan synthase beta chain from Caulobacter crescentus (406 aa), FASTA scores: opt: 1662, E(): 0, (60.6% identity in 404 aa overlap). Belongs to the TrpB family. Tetramer of two alpha and two beta chains. P9WFX9 No intermediary metabolism and respiration 2O2E,2O2J P66984 P9WFX9 GO:0000162,GO:0004834,GO:0030170 4.2.1.20 P9WFX9 NC_000962.3 Mycobrowser_v5 CDS 1819963 1821693 . - 0 Rv1620c cydC Involved in active transport across the membrane of component linked with the assembly of cytochrome: involved in cytochrome biogenesis (aerobic respiration). Responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane. Probable 'component linked with the assembly of cytochrome' transport transmembrane ATP-binding protein ABC transporter CydC Rv1620c, (MTCY01B2.12c), len: 576 aa. Probable cydC, transmembrane ATP-binding protein ABC transporter involved in transport of component linked with the assembly of cytochrome (see citation below), similar to others e.g. CYDC_ECOLI|P23886 transport ATP-binding protein from Escherichia coli (573 aa), FASTA scores: opt: 631, E(): 1.6e-30, (28.5% identity in 569 aa overlap); C-terminal part of AL034355|SCD78_14 from Streptomyces coelicolor (1172 aa), FASTA scores: opt: 956, E(): 0, (38.8% identity in 554 aa overlap); etc. Contains (PS00211) ABC transporters family signature, and (PS00017) ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters). O06137 No intermediary metabolism and respiration O06137 O06137 GO:0042626,GO:0016021,GO:0055085,GO:0005524 O06137 NC_000962.3 Mycobrowser_v5 CDS 1824430 1825887 . - 0 Rv1623c cydA Involved in the respiratory chain (at the terminal step): aerobic respiration. Cytochrome D terminal oxidase complex is the component of the aerobic respiratory chain that is supposedly predominant when cells are grown at low aeration [catalytic activity: ubiquinol-8 + O(2) = ubiquinone-8 + H(2)O]. Probable integral membrane cytochrome D ubiquinol oxidase (subunit I) CydA (cytochrome BD-I oxidase subunit I) Rv1623c, (MTCY01B2.15c), len: 485 aa. Probable cydA (previously known as appC, but renamed cydA to conform with Mycobacterium smegmatis nomenclature), cytochrome D ubiquinol oxidase subunit I, integral membrane protein, similar to others e.g. P26459|APPC_ECOLI|CYXA|CBDA|B0978 cytochrome BD-II oxidase subunit I from Escherichia coli strain K12 (514 aa), FASTA scores: opt: 870, E(): 0, (35.9% identity in 485 aa overlap); AL034355|SCD78_12 from Streptomyces coelicolor (501 aa), FASTA scores: opt: 1099, E(): 0, (48.6% identity in 510 aa overlap); etc. L7N662 No intermediary metabolism and respiration Q7D892 L7N662 GO:0055114,GO:0016491,GO:0016020 1.10.3.- NC_000962.3 Mycobrowser_v5 CDS 1826614 1827945 . - 0 Rv1625c cya Involved in cAMP synthesis [catalytic activity: ATP = 3',5'-cyclic AMP + diphosphate]. Inhibited by polyphosphates. Membrane-anchored adenylyl cyclase Cya (ATP pyrophosphate-lyase) (adenylate cyclase) Rv1625c, (MT1661, MTCY01B2.17c), len: 443 aa. Cya, membrane-anchored adenylyl cyclase (see citations below). C-terminal half is similar to region in numerous eukaryotic adenylate and guanylate cyclases. N-terminal half hydrophobic. FASTA score: CYG2_RAT|P22717 guanylate cyclase soluble, beta-2 chain (682 aa), FASTA scores: opt: 552, E(): 2.7e-26, (40.3% identity in 226 aa overlap). Some similarity to Rv2435c|MTCY428.11 from Mycobacterium tuberculosis (730 aa), E(): 7e-19. Start changed since first submission (+25 aa). Belongs to adenylyl cyclase class-4/guanylyl cyclase family. P9WQ35 No intermediary metabolism and respiration 1YK9 P0A4Y0 P9WQ35 GO:0004016,GO:0006171,GO:0016021,GO:0046872,GO:0005886,GO:0005524 4.6.1.1 P9WQ35 NC_000962.3 Mycobrowser_v5 CDS 1830665 1833379 . + 0 Rv1629 polA Involved in post-incision events. In addition to DNA polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [catalytic activity: N deoxynucleoside triphosphate = N diphosphate + {DNA}(N)]. Probable DNA polymerase I PolA Rv1629, (MTCY01B2.21), len: 904 aa. Probable polA, DNA polymerase I (see citations below). Has DNA polymerase family a signature (PS00447) at C-terminal end. FASTA best: DPO1_MYCTU|Q07700 DNA polymerase I from Mycobacterium tuberculosis (904 aa). Some similarity to Rv2090|MTCY49.30 (393 aa), E(): 2.2e-18, (38.7% identity in 292 aa overlap). Belongs to DNA polymerase type-a family. P9WNU5 No information pathways P0A550 P9WNU5 GO:0008409,GO:0003677,GO:0006281,GO:0006260,GO:0003887,GO:0005622,GO:0008408 2.7.7.7 P9WNU5 NC_000962.3 Mycobrowser_v5 CDS 1833542 1834987 . + 0 Rv1630 rpsA Binds mRNA; thus facilitating recognition of the initiation point. It is needed to translate mRNA with a short shine-dalgarno (SD) purine-rich sequence. 30S ribosomal protein S1 RpsA Rv1630, (MTCY01B2.22), len: 481 aa. rpsA, 30S ribosomal protein S1. FASTA best: RS1_MYCLE|P46836 30s ribosomal protein S1 from Mycobacterium leprae (482 aa), opt: 2655, E(): 0, (87.2% identity in 483 aa overlap). P9WH43 No information pathways O06147 P9WH43 GO:0005840,GO:0003735,GO:0006412,GO:0003723 P9WH43 NC_000962.3 Mycobrowser_v5 CDS 1837075 1839171 . + 0 Rv1633 uvrB Involved in nucleotide excision repair. The ABC excision nuclease is a DNA repair enzyme that catalyzes the excision reaction of UV-damaged nucleotide segments producing oligomers having the modified base(S). UVRB stimulates the ATPase activity of UVRA in the presence of UV-irradiated double-stranded DNA. It also enhances the ability of UVRA to bind to UV-irradiated duplex DNA Probable excinuclease ABC (subunit B - helicase) UvrB Rv1633, (MTCY01B2.25), len: 698 aa. Probable uvrB, excinuclease ABC, subunit B; helicase (see Mizrahi & Andersen 1998; Sancar 1994); has ATP/GTP-binding site motif A (P-loop; PS00017) near N-terminus (see citation below). FASTA best: UVRB_MICLU|P10125 from Micrococcus luteus (709 aa), opt: 3268, E(): 0, (71.3% identity in 704 aa overlap). Also similar to Mycobacterium tuberculosis Rv2973c (recG); and Rv1020 (mfd). Belongs to the UVRB family. P9WFC7 No information pathways P67422 P9WFC7 GO:0003677,GO:0009432,GO:0005737,GO:0009381,GO:0004386,GO:0006289,GO:0005524 P9WFC7 NC_000962.3 Mycobrowser_v5 CDS 1852273 1852878 . + 0 Rv1641 infC If-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins. Probable initiation factor if-3 InfC Rv1641, (MTCY06H11.05), len: 201 aa. Probable infC, initiation factor if-3, similar to many e.g. IF3_BACST|P03000 initiation factor if-3 from Bacillus stearothermophilus (171 aa), FASTA scores: opt: 560, E(): 1.9e-27, (50.6% identity in 166 aa overlap). Note that an AUC initiation codon has been used, the Bacillus (IF3_BACSU) and Escherichia coli (IF3_ECOLI) proteins use an AUU initiation codon, and the Myxococcus xanthus (DSG_MYXXA) homolog uses a AUC. Belongs to the if-3 family. P9WKJ9 No information pathways P65135 P9WKJ9 GO:0003743,GO:0006413,GO:0005737 P9WKJ9 NC_000962.3 Mycobrowser_v5 CDS 1859758 1862253 . + 0 Rv1650 pheT Charging PHE-tRNA [catalytic activity: ATP + L-phenylalanine + tRNA(PHE) = AMP + diphosphate + L-phenylalanyl-tRNA(PHE)] Probable phenylalanyl-tRNA synthetase, beta chain PheT Rv1650, (MTCY06H11.15), len: 831 aa. Probable pheT, Phenylalanyl-tRNA synthetase beta chain, similar to several e.g. SYFB_ECOLI|P07395 from Escherichia coli (795 aa), FASTA scores: opt: 995, E(): 0, (31.8% identity in 847 aa overlap). Belongs to the phenylalanyl-tRNA synthetase beta chain family - subfamily 1. P9WFU1 No information pathways P94985 P9WFU1 GO:0005737,GO:0000287,GO:0004826,GO:0006432,GO:0000049,GO:0008033,GO:0005524 6.1.1.20 P9WFU1 NC_000962.3 Mycobrowser_v5 CDS 1862347 1865382 . - 0 Rv1651c PE_PGRS30 Function unknown. Thought to be involved in virulence. PE-PGRS family protein PE_PGRS30 Rv1651c, (MTCY06H11.16c), len: 1011 aa. PE_PGRS30, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citations below), similar to many e.g. Q10637|Y03A_MYCTU hypothetical glycine-rich 49.6 kd protein (603 aa), FASTA scores: opt: 1757, E(): 0, (50.8% identity in 714aa overlap). The transcription of this CDS seems to be activated in macrophages (see Ramakrishnan et al., 2000). Q79FL8 No PE/PPE Q79FL8 Q79FL8 Q79FL8 NC_000962.3 Mycobrowser_v5 CDS 1870842 1871354 . + 0 Rv1657 argR Regulates arginine biosynthesis genes Probable arginine repressor ArgR (AHRC) Rv1657, (MTCY06H11.22), len: 170 aa. Probable argR, Arginine repressor (alternate gene name: ahrC). Similar to AHRC_BACSU|P17893 arginine hydroximate resistance protein from Bacillus subtilis (149 aa), FASTA scores: opt: 283, E(): 1.8e-11, (34.5% identity in 142 aa overlap); and ARGR_ECOLI|P15282 arginine repressor from Escherichia coli (156 aa), FASTA scores: opt: 194, E(): 6.4e-06, (30.8% identity in 146 aa overlap). Belongs to the ArgR family. P9WPY9 No regulatory proteins 2ZFZ,3BUE,3CAG,3ERE,3FHZ,3LAJ,3LAP P0A4Y8 P9WPY9 GO:0005737,GO:0006355,GO:0006350,GO:0003700,GO:0006526 P9WPY9 NC_000962.3 Mycobrowser_v5 CDS 1886512 1888020 . + 0 Rv1663 pks17 Potentially involved in some intermediate steps for the synthesis of a polyketide molecule which may be involved in secondary metabolism Probable polyketide synthase Pks17 Rv1663, (MTCY275.02), len: 502 aa. Probable pks17, polyketide synthase, similar to other polyketide synthases e g. ERY2_SACER|Q03132 erythronolide synthase, modules 3 and 4 (3567 aa) from Saccharopolyspora erythraea (Streptomyces erythraeus), FASTA scores: opt: 1207, E(): 0, (43.9% identity in 531 aa overlap). Also similar to other Mycobacterium tuberculosis probable polyketide synthases e.g. pks7 and pks1. Note that the similarity extends into the upstream ORF Rv1662 (MTCY275.01) and this could be accounted for by a frameshift, although the sequence has been checked and no discrepancy was found. Contains PS00012 Phosphopantetheine attachment site. O06585 No lipid metabolism O06585 O06585 GO:0009058,GO:0048037,GO:0016491,GO:0031177,GO:0000036 O06585 NC_000962.3 Mycobrowser_v5 CDS 1891226 1892287 . + 0 Rv1665 pks11 Possibly involved in the biosynthesis of secondary metabolites [catalytic activity: 3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2] Chalcone synthase Pks11 Rv1665, (MTCY275.04-MTV047.01), len: 353 aa. pks11, chalcone synthase, some similarity to BCSA_BACSU|P54157 putative chalcone synthase from Bacillus subtilis (365 aa), FASTA scores: opt: 615, E(): 6.2e-32, (33.4% identity in 308 aa overlap); and to many plant chalcone synthases e.g. CHS_VIGUN|P51089 chalcone synthase (388 aa), FASTA scores: opt: 391, E(): 7.8e-18, (27.2% identity in 349 aa overlap). Highly similar to upstream ORF Rv1660|MTCY06H11.25 pks10 (72.7% identity in 308 aa overlap); and Rv1372 pks18. P9WPF3 No lipid metabolism O06587 P9WPF3 GO:0009058,GO:0016210,GO:0008415 2.3.1.74 P9WPF3 NC_000962.3 Mycobrowser_v5 CDS 1896876 1898207 . - 0 Rv1672c Rv1672c Thought to be involved in transport across the membrane. Responsible for the translocation of undetermined substrate across the membrane. Probable conserved integral membrane transport protein Rv1672c, (MTV047.07c), len: 443 aa. Probable conserved integral membrane transport protein, major facilitator superfamily, similar to several phthalate transporters or tartrate transporters e.g. U25634|AVU25634_2 Agrobacterium vitis plasmid pTrAB (433 aa), FASTA scores: opt: 914, E(): 0, (37.1% identity in 426 aa overlap); etc. O53919 No cell wall and cell processes O53919 O53919 GO:0055085 NC_000962.3 Mycobrowser_v5 CDS 1903299 1904420 . + 0 Rv1679 fadE16 Function unknown, but involvement in lipid degradation. Possible acyl-CoA dehydrogenase FadE16 Rv1679, (MTV047.14, MTCI125.01), len: 373 aa. Possible fadE16, acyl-CoA dehydrogenase, similar to acyl/butyryl-CoA dehydrogenases e.g. NP_244665.1|NC_002570 acyl-CoA dehydrogenase from Bacillus halodurans (380 aa); NP_000008.1|NM_000017 acyl-Coenzyme A dehydrogenase from Homo sapiens (412 aa); Z99113|BSUB0010_119 from Bacillus subtilis (380 aa), FASTA scores: opt: 439, E(): 3.4e-20, (29.6% identity in 287 aa overlap); etc. Weakly similar to many dehydrogenases and to P31571|CAIA_ECOLI probable carnitine operon oxidoreductase from Escherichia coli (380 aa), FASTA scores: opt: 109, E(): 0.0066, (28.6% identity in 98 aa overlap). O53926 No lipid metabolism O53926 O53926 GO:0003995,GO:0055114,GO:0050660 1.3.99.- O53926 NC_000962.3 Mycobrowser_v5 CDS 1907594 1910593 . + 0 Rv1683 Rv1683 Supposed involvement in lipid biosynthesis and degradation Possible bifunctional enzyme; long-chain acyl-CoA synthase and lipase. Rv1683, (MTCI125.05), len: 999 aa. Possible bifunctional long-chain acyl-CoA synthase and lipase. Equivalent to Z95117|MLCB1351_21 possible long-chain acyl-CoA synthase from Mycobacterium leprae (1002 aa) (85.6% identity in 1002 aa overlap). Weakly similar to FATP_MOUSE|Q60714 long-chain fatty acid transport protein (646 aa), fasta scores: opt: 331, E(): 5e-08, (24.8% identity in 630 aa overlap). Also similar to O35488|AF033031 Mouse very-long-chain acyl-CoA synthetase (620 aa), fasta scores: opt: 435, E(): 2.2e-12, (24.8% identity in 545 aa overlap). Weakly similar to Mycobacterium tuberculosis protein MTCI364.18 (27.4% identity in 583 aa overlap). Contains PS00120 Lipases, serine active site. O33185 No lipid metabolism O33185 O33185 GO:0008152,GO:0003824 O33185 NC_000962.3 Mycobrowser_v5 CDS 1912153 1912920 . - 0 Rv1687c Rv1687c Thought to be involved in active transport of undetermined substrate (possibly drug) across the membrane. Responsible for energy coupling to the transport system. Probable conserved ATP-binding protein ABC transporter Rv1687c, (MTCI125.09c), len: 255 aa. Probable conserved ATP-binding protein ABC transporter (see citation below), similar to many ABC-type transporters e.g. P55476|NODI_RHISN nodulation ATP-binding protein I from Rhizobium sp. (343 aa), FASTA scores: opt: 479, E(): 3.7e-23, (34.6% identity in 243 aa overlap); etc. Also similar to many other Mycobacterium tuberculosis ABC-type transporters e.g. MTCY19H9.04 (34.5% identity in 238 aa overlap). Contains PS00211 ABC transporters family signature and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters). Also contains PS00039 dead-box subfamily ATP-dependent helicases signature, though this may be spurious. O33189 No cell wall and cell processes O33189 O33189 GO:0016887,GO:0005524 O33189 NC_000962.3 Mycobrowser_v5 CDS 1917940 1918746 . + 0 Rv1694 tlyA Methylates 16S and 23S rRNA. Also has contact-dependent haemolytic activity; possibly involved in virulence (pore formation). 2'-O-methyltransferase TlyA Rv1694, (MTCI125.16), len: 268 aa. TlyA, 2'-O-methyltransferase; cytotoxin/haemolysin homologue (see citations below), almost identical to NP_301968.1|NC_002677 cytotoxin/haemolysin homologue TlyA from Mycobacterium leprae (269 aa). TlyA homologues were also identified by PCR in Mycobacterium avium, Mycobacterium bovis BCG, but appeared absent in M. smegmatis, M. vaccae, M. kansasii, M. chelonae and M. phlei (see Wren et al., 1998). Also highly similar to CAB83047.1|AJ271681 putative haemolysin from Mycobacterium ulcerans (281 aa); and similar to HLYA_TREHY|Q06803 pore-forming haemolysin/cytotoxin virulence determinant from Treponema hyodysenteriae (240 aa), FASTA scores: opt: 514, E():3e-30, (37.3% identity in 236 aa overlap). P9WJ63 No virulence, detoxification, adaptation Q50760 P9WJ63 GO:0019836,GO:0032259,GO:0008168,GO:0003723 AMI P9WJ63 NC_000962.3 Mycobrowser_v5 CDS 1929131 1929721 . - 0 Rv1703c Rv1703c Catalyzes the O-methylation [catalytic activity: S-adenosyl-L-methionine + catechol = S-adenosyl-L-homocysteine + guaiacol] Probable catechol-O-methyltransferase Rv1703c, (MTCI125.25c), len: 196 aa. Probable catechol-o-methyltransferase, most similar to COMT_HUMAN|P21964 soluble form of mammalian catechol o-methyltransferase (271 aa), FASTA scores: opt: 405, E(): 7.8e-29, (38.9% identity in 190 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical methyltransferases Rv0187, Rv1220c. L0TAD5 No intermediary metabolism and respiration O33202 L0TAD5 GO:0016206 2.1.1.6 L0TAD5 NC_000962.3 Mycobrowser_v5 CDS 1929786 1931456 . - 0 Rv1704c cycA Permease that is involved in the transport across the cytoplasmic membrane of D-alanine, D-serine and glycine Probable D-serine/alanine/glycine transporter protein CycA Rv1704c, (MTCI125.26c), len: 556 aa. Probable cycA, D-serine/D-alanine/glycine transporter, highly similar to P39312|CYCA_ECOLI d-serine/d-alanine/glycine transporter from Escherichia coli (470 aa), FASTA scores: opt: 1906, E(): 0, (59.3% identity in 459 aa overlap); etc. Also similar to other Mycobacterium tuberculosis amino-acid permeases e.g. Rv2127, Rv0346c, etc. Contains PS00218 amino acid permeases signature. Belongs to the amino acid permease family (APC family). O33203 No cell wall and cell processes O33203 O33203 GO:0006865,GO:0016021,GO:0055085,GO:0015171 O33203 NC_000962.3 Mycobrowser_v5 CDS 1934882 1936342 . + 0 Rv1707 Rv1707 Unknown; possibly involved in transport of sulfate across the membrane. Probable conserved transmembrane protein Rv1707, (MTCI125.29), len: 486 aa. Probable conserved transmembrane protein, possibly involved in transport of sulfate, similar to several hypothetical proteins belonging to the sulfate permease family e.g. P40877|YCHM_ECOLI hypothetical 58.4 kDa protein in pth-prsa intergenic region from Escherichia coli (550 aa), FASTA scores: opt: 486, E(): 0, (33.1% identity in 492 aa overlap). Also similar to many other Mycobacterium tuberculosis membrane proteins e.g. Rv3273, Rv1739c. Seems to belong to the SulP family. O33206 No cell wall and cell processes O33206 O33206 GO:0055085,GO:0005215,GO:0016021 O33206 NC_000962.3 Mycobrowser_v5 CDS 1942659 1943573 . + 0 Rv1715 fadB3 Thought to be involved in fatty acid degradation. FADB and FADA are the alpha and beta subunits of the multifunctional enzyme complex of the fatty acid degradation cycle [catalytic activity: (S)-3-hydroxybutanoyl-CoA + NADP+ = 3-acetoacetyl-CoA + NADPH]. Probable 3-hydroxybutyryl-CoA dehydrogenase FadB3 (beta-hydroxybutyryl-CoA dehydrogenase) (BHBD) Rv1715, (MTV048.02), len: 304 aa. Probable fadB3, 3-hydroxybutyryl-CoA dehydrogenase, highly similar to many e.g. NP_107236.1|NC_002678 3-hydroxybutyryl-CoA dehydrogenase from Mesorhizobium loti (309 aa); NP_250319.1|NC_002516 probable 3-hydroxyacyl-CoA dehydrogenase from Pseudomonas aeruginosa (509 aa); P45856|HBD_BACSU probable 3-hydroxybutyryl-CoA dehydrogenase from Bacillus subtilis (287 aa), FASTA scores: opt: 488, E(): 1.5e-24, (38.7% identity in 279 aa overlap); etc. Could belong to the 3-hydroxyacyl-CoA dehydrogenase family. L7N688 No lipid metabolism Q7D836 L7N688 GO:0050662,GO:0006631,GO:0055114,GO:0008691 1.1.1.157 L7N688 NC_000962.3 Mycobrowser_v5 CDS 3004745 3005650 . - 0 Rv2688c Rv2688c Thought to be involved in active transport of unidentified antibiotic across the membrane (export): antibiotic resistance by an export mechanism. Responsible for energy coupling to the transport system. Antibiotic-transport ATP-binding protein ABC transporter Rv2688c, (MTCY05A6.09c), len: 301 aa. Antibiotic-transport ATP-binding protein ABC transporter (see citation below), highly similar to AAK47077|MT2762 ABC transporter ATP-binding protein from Mycobacterium tuberculosis strain CDC1551 (317 aa), FASTA scores: opt: 1714, E(): 5.1e-93, (95.6% identity in 274 aa overlap). Also highly similar to other ATP-binding proteins ABC transporter e.g. Q9K639|BH3893 from Bacillus halodurans (282 aa), FASTA scores: opt: 644, E(): 1.4e-30, (38.% identity in 285 aa overlap); O58550|PH0820 from Pyrococcus horikoshii (312 aa), FASTA scores: opt: 574, E(): 1.8e-26, (39.1% identity in 307 aa overlap); Q9WYM0|TM0389 from Thermotoga maritima (301 aa), FASTA scores: opt: 536, E(): 2.9e-24, (36.1% identity in 291 aa overlap); etc. Has ATP/GTP-binding site motif A (P-loop) at N-terminus (PS00017). Belongs to the ATP-binding transport protein family (ABC transporters). P9WQL7 No cell wall and cell processes O07190 P9WQL7 GO:0016887,GO:0005886,GO:0046677,GO:0006810,GO:0005524 3.6.3.- P9WQL7 NC_000962.3 Mycobrowser_v5 CDS 1954631 1955569 . - 0 Rv1729c Rv1729c Possible methyltransferase Possible S-adenosylmethionine-dependent methyltransferase Rv1729c, (MTCY04C12.14c), len: 312 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), similar to many Mycobacterium tuberculosis proteins e.g. Q50726|Rv3399|YX99_MYCTU (348 aa), FASTA scores: opt: 1019, E(): 0, (55.7% identity in 296 aa overlap); P95074|Rv0726c (367 aa), O53795|Rv0731c (318 aa), and O53841|Rv0830 (301 aa), etc. P9WFH9 No lipid metabolism P71987 P9WFH9 GO:0008168 2.1.1.- P9WFH9 NC_000962.3 Mycobrowser_v5 CDS 1957677 1959233 . + 0 Rv1731 gabD2 Involved in 4-aminobutyrate (GABA) degradation pathway [catalytic activity: succinate semialdehyde + NAD(P)(+) + H(2)O = succinate + NAD(P)H]. Possible succinate-semialdehyde dehydrogenase [NADP+] dependent (SSDH) GabD2 Rv1731, (MTCY04C12.16), len: 518 aa. Possible gabD2, succinate-semialdehyde dehydrogenase [NADP+] dependent, similar to others e.g. GABD_ECOLI|P25526 succinate-semialdehyde dehydrogenase from Escherichia coli (482 aa), FASTA scores: opt: 870, E(): 0, (34.7% identity in 449 aa overlap); etc. Also similar to gabD1|Rv0234c|MTCY08D5.30c probable succinate-semialdehyde dehydrogenase [NADP+] dependent from Mycobacterium tuberculosis (511 aa); and other semialdehyde dehydrogenases e.g. Rv0768|aldA (489 aa), Rv2858c|aldC (455 aa), etc. Contains PS00216 Sugar transport proteins signature 1, PS00687 Aldehyde dehydrogenases glutamic acid active site. Belongs to the aldehyde dehydrogenases family. Note that previously known as gabD1. P9WNX7 No intermediary metabolism and respiration P96417 P9WNX7 GO:0009013,GO:0055114 1.2.1.16 P9WNX7 NC_000962.3 Mycobrowser_v5 CDS 1962228 1964186 . - 0 Rv1736c narX Involved in nitrate reduction, and in the persistence in the host [catalytic activity: nitrite + acceptor = nitrate + reduced acceptor] Probable nitrate reductase NarX Rv1736c, (MTCY04C12.21c), len: 652 aa. Probable narX, nitrate reductase. Contains three domains: N-terminus (250 aa) is similar to e.g. N-terminus of NARG_ECOLI|P09152 respiratory nitrate reductase 1 alpha chain from Escherichia coli (1246 aa), FASTA scores: E(): 0, (58.6% identity in 251 aa overlap); and Rv1161|MTCI65.28|NARG probable respiratory nitrate reductase (alpha chain) from Mycobacterium tuberculosis (1232 aa). Central region (260-410 aa) is similar to Rv1163|O06561|NARJ probable respiratory nitrate reductase (delta chain) from Mycobacterium tuberculosis (201 aa), FASTA scores: E(): 0, (64.2% identity in 159 aa overlap). C-terminus (420 aa-) is similar to Rv1164|O06562|NARI probable respiratory nitrate reductase (gamma chain) from Mycobacterium tuberculosis (246 aa), FASTA scores: E(): 0, (68.6% identity in 239 aa overlap). Contains PS00551 Prokaryotic molybdopterin oxidoreductases signature 1. P9WJQ1 No intermediary metabolism and respiration P71994 P9WJQ1 GO:0009055,GO:0022900,GO:0016021,GO:0046872,GO:0042128,GO:0008940,GO:0009325,GO:0005886,GO:0006810,GO:0051539 1.7.99.4 P9WJQ1 NC_000962.3 Mycobrowser_v5 CDS 1964183 1965370 . - 0 Rv1737c narK2 Involved in excretion of nitrite, produced by the dissimilatory reduction of nitrate, across the membrane. Responsible for the translocation of the substrate across the membrane. Possible nitrate/nitrite transporter NarK2 Rv1737c, (MTCY04C12.22c), len: 395 aa. Possible narK2, nitrate/nitrite-transport integral membrane protein (see Hutter & Dick 2000), possibly member of major facilitator superfamily (MFS), similar to P46907|NARK_BACSU nitrite extrusion protein from Bacillus subtilis (395 aa), FASTA scores: opt: 742, E(): 0, (33.6% identity in 375 aa overlap); and to AL109989|SCJ12.23 hypothetical nitrate/nitrite transporter from Streptomyces coelicolor (412 aa), FASTA scores: opt: 1181, E(): 0, (49.4% identity in 389 aa overlap). P9WJY7 No cell wall and cell processes P71995 P9WJY7 GO:0042128,GO:0005886,GO:0055085,GO:0016021 P9WJY7 NC_000962.3 Mycobrowser_v5 CDS 1965955 1967637 . - 0 Rv1739c Rv1739c Involved in sulphate transport across the membrane. Responsible for the translocation of the substrate across the membrane. Probable sulphate-transport transmembrane protein ABC transporter Rv1739c, (MTCY04C12.24c, MTCY28.01), len: 560 aa. Probable sulphate-transport transmembrane protein ABC transporter, similar to several e.g. P53392|G607186 high affinity sulphate transporter from Stylosanthes hamata (662 aa), FASTA scores: opt: 382, E(): 1.6e-16, (28.0% identity in 564 aa overlap); U59234.1|AAB88215.1 biotin carb. from Synechococcus sp. PCC 7942 (574 aa), FASTA scores: opt: 1838, E(): 0, (50.0% identity in 550 aa overlap); etc. Contains PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters), and seems to belong to the SULP family. P9WGF7 No cell wall and cell processes P71997 P9WGF7 GO:0005886,GO:0008271,GO:0008272,GO:0055085,GO:0016021 P9WGF7 NC_000962.3 Mycobrowser_v5 CDS 3034635 3034883 . + 0 Rv2722 Rv2722 Function unknown Conserved hypothetical protein Rv2722, (MTCY154.02), len: 82 aa. Conserved hypothetical protein, similar to Q9CCB9|ML1001 hypothetical protein from Mycobacterium leprae (91 aa), FASTA scores: opt: 154, E(): 0.00053, (37.5% identity in 88 aa overlap). Equivalent to AAK47111 from Mycobacterium tuberculosis strain CDC1551 (94 aa) but shorter 12 aa. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O33227 No conserved hypotheticals O33227 NC_000962.3 Mycobrowser_v5 CDS 1969004 1970704 . + 0 Rv1743 pknE Involved in signal transduction (via phosphorylation). Thought to be involved in membrane transport [catalytic activity: ATP + a protein = ADP + a phosphoprotein]. Probable transmembrane serine/threonine-protein kinase E PknE (protein kinase E) (STPK E) Rv1743, (MTCY28.05,MTCY04C12.28), len: 566 aa. Probable pknE, transmembrane serine/threonine protein kinase (see citation below), similar to PKN1_MYXXA|P33973 serine/threonine-protein kinase pkn1 (693 aa), fasta scores: opt: 542, E(): 1.1e-19, (35.8% identity in 302 aa overlap). Also highly similar to K08G_MYCTU|Q11053 probable serine/threonine-protein kinase (626 aa) (59.8% identity in 381 aa overlap). Contains PS00107 Protein kinases ATP-binding region signature. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases. P9WI77 No regulatory proteins 2H34 P72001 P9WI77 GO:0016021,GO:0005886,GO:0006468,GO:0004674,GO:0005524 2.7.11.1 P9WI77 NC_000962.3 Mycobrowser_v5 CDS 1977969 1979567 . - 0 Rv1750c fadD1 Function unknown, but supposed involvement in lipid degradation. Possible fatty-acid-CoA ligase FadD1 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv1750c, (MTCY28.13c, MTCY04C12.34), len: 532 aa. Possible fadD1, fatty-acid-CoA synthetase, similar in part to others e.g. O35488|VLCS_MOUSE very-long-chain acyl-CoA synthetase from Mus musculus (620 aa); NP_113924.1|NM_031736 solute carrier family 27 (fatty acid transporter) member 2 from Rattus norvegicus (620 aa); NP_459076.1|NC_003197 crotonobetaine/carnitine-CoA ligase from Salmonella typhimurium (517 aa); CAIC_ECOLI|P31552 probable crotonobetaine/carnitine-CoA ligase from Escherichia coli (522 aa), FASTA scores: opt: 448, E(): 1.9e-21, (25.1% identity in 502 aa overlap); etc. Also highly similar to fadD17|Rv3506|MTV023.13 probable fatty-acid-CoA ligase from Mycobacterium tuberculosis (502 aa); and similar to others from Mycobacterium tuberculosis e.g. fadD6|MTCI364.18|Rv1206|O05307 probable fatty-acid-CoA ligase (597 aa), FASTA score: (28.3% identity in 519 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family. P72007 No lipid metabolism P72007 P72007 GO:0008152,GO:0016874 6.2.1.- P72007 NC_000962.3 Mycobrowser_v5 CDS 1988680 1989006 . - 0 Rv1757c Rv1757c Required for the transposition of the insertion element IS6110. Putative transposase for insertion sequence element IS6110 (fragment) Rv1757c, (MTCY28.23c), len: 108 aa. Putative Transposase for IS6110 (fragment), identical to many other Mycobacterium tuberculosis IS6110 transposase subunits e.g. Q50686|YIA4_MYCTU Insertion element IS6110 hypothetical 12.0 kDa protein (108 aa), fasta scores: E(): 1.4e-43, (100.00% identity in 108 aa overlap). The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv1756c and Rv1757c, the sequence UUUUAAAG (directly upstream of Rv1756c) maybe responsible for such a frameshifting event (see McAdam et al., 1990). P9WKH5 No insertion seqs and phages P0C5G9 P9WKH5 GO:0004803,GO:0006313,GO:0003677 P9WKH5 NC_000962.3 Mycobrowser_v5 CDS 3430387 3430710 . + 0 Rv3065 mmr Involved in transport of multidrugs (tetraphenylphosphonium, erythromycin, ethidium bromide, acriflavine, safranin O, pyronin Y, etc) across the membrane (export): multidrugs resistance by an export mechanism (conferes resistance to toxic compounds by removing them for the cells). Responsible for the translocation of the substrate across the membrane. Multidrugs-transport integral membrane protein Mmr Rv3065, (MT3150.1, MTCY22D7.17c), len: 107 aa. Mmr, integral membrane multidrugs resistance transporter (see citation below), equivalent to Q9CBP1|ML1756 probable multidrug resistance protein from Mycobacterium leprae (107 aa), FASTA scores: opt: 534, E(): 3.3e-28, (77.55% identity in 107 aa overlap). Also highly similar to bacterial proteins involved in resistance to ethidium bromide or methyl viologen e.g. O87866|QACG_STASP quaternary ammonium compound-resistance protein QACG (quarternary ammonium determinant G) from Staphylococcus sp. strain ST94 (107 aa), FASTA scores: opt: 307, E(): 1.8e-13, (39.8% identity in 103 aa overlap); P96460|QAC quaternary ammonium compounds resistance protein QAC from Staphylococcus aureus (107 aa), FASTA scores: opt: 304, E(): 2.8e-13, (40.4% identity in 104 aa overlap); Q57225|QACE_ECOLI quaternary ammonium compound-resistance protein QACE (quarternary ammonium determinant E) from Escherichia coli (110 aa), FASTA scores: opt: 300, E(): 5.2e-13, (48.15% identity in 108 aa overlap); AAG55967|Z1870 methylviologen resistance protein encoded within prophage CP-933X from Escherichia coli strain O157:H7 EDL933 (110 aa); P23895|EMRE|MVRC|EB|B0543 EMRE protein from Escherichia coli (110 aa), FASTA scores: opt: 290, E(): 2.3e-12, (43.55% identity in 101 aa overlap); etc. Also similar to the SugE protein of enteric bacteria. Belongs to the small multidrug resistance (SMR) protein family. Note that previously known as emrE. P9WGF1 No cell wall and cell processes 2IQ4 P69926 P9WGF1 GO:0005886,GO:0046677,GO:0006810,GO:0016021 P9WGF1 NC_000962.3 Mycobrowser_v5 CDS 1989833 1992577 . - 0 Rv1759c wag22 Function unknown. Has fibronectin-binding activity (could thus mediate bacterial attachment to host cells). Thought to be expressed during infection. PE-PGRS family protein Wag22 Rv1759c, (MT1807, MTCY28.25c), len: 914 aa. Wag22, antigen member (see citations below) of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins, highly similar to others e.g. MT1367|Q10637 hypothetical glycine-rich 49.6 kDa protein from Mycobacterium tuberculosis (603 aa), FASTA scores: opt: 2010, E(): 0, (53.0% identity in 724 aa overlap); etc. P9WIG5 No PE/PPE P0A686 P9WIG5 P9WIG5 NC_000962.3 Mycobrowser_v5 CDS 1996427 1997413 . + 0 Rv1764 Rv1764 Required for the transposition of the insertion element IS6110. Putative transposase Rv1764, (MTCY28.30), len: 328 aa. Putative Transposase for IS6110 insertion element. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv1763 and Rv1764, the sequence UUUUAAAG (directly upstream of Rv1764) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 34 aa) P9WKH9 No insertion seqs and phages P0C5G8 P9WKH9 GO:0015074,GO:0006310,GO:0032196,GO:0003677 P9WKH9 NC_000962.3 Mycobrowser_v5 CDS 2005161 2006447 . + 0 Rv1771 Rv1771 Possibly involved in biosynthesis of L-ascorbic acid (vitamin C). Oxidizes L-gulono-1,4-lactone. L-gulono-1,4-lactone dehydrogenase Rv1771, (MTCY28.37), len: 428 aa. L-gulono-1,4-lactone dehydrogenase (See Wolucka and Communi, 2006), similar to e.g. GGLO_RAT|P10867 l-gulonolactone oxidase (439 aa), FASTA scores: opt: 862, E(): 0, (34.1% identity in 434 aa overlap). Also shows slight similarity to Mycobacterium tuberculosis oxidoreductase Rv1726|MTCY04C12.11 (22.9% identity in 441 aa overlap) and others e.g. Rv3107c, Rv1257c, Rv2251, etc. Contains PS00862 Oxygen oxidoreductases covalent FAD-binding site. Alternative nucleotide at position 2006032 (a->G; Q291R) has been observed. P9WIT3 No intermediary metabolism and respiration O06804 P9WIT3 GO:0050660,GO:0016020,GO:0055114,GO:0003885 1.-.-.- P9WIT3 NC_000962.3 Mycobrowser_v5 CDS 2010656 2011960 . + 0 Rv1777 cyp144 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Probable cytochrome P450 144 Cyp144 Rv1777, (MT1827, MTCY25C11.04), len: 434 aa. Probable cyp144, cytochrome p450, similar to CPXM_BACME|Q06069 cytochrome p450 (meg) (410 aa), FASTA scores: opt: 435 E(): 2.3e-16, (28.8% identity in 372 aa overlap). Also similar to several other Mycobacterium tuberculosis p450 genes including Rv0766c, Rv2266, etc. Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. Belongs to the cytochrome P450 family. P9WPL1 No intermediary metabolism and respiration O33180 P9WPL1 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPL1 NC_000962.3 Mycobrowser_v5 CDS 2015302 2017476 . - 0 Rv1781c malQ Transfers a segment of a (1,4)-alpha-D-glucan to a new 4-position in an acceptor, which may be glucose or (1,4)-alpha-D-glucan Probable 4-alpha-glucanotransferase MalQ (amylomaltase) (disproportionating enzyme) (D-enzyme) Rv1781c, (MTV049.03c), len: 724 aa. Probable malQ, 4-alpha-glucanotransferase, similar to many, e.g. P15977|MALQ_ECOLI 4-alpha-glucanotransferase (694 aa), FASTA scores: opt: 964, E(): 0, (31.8% identity in 694 aa overlap). Belongs to the disproportionating enzyme family. P9WK23 No intermediary metabolism and respiration P65336 P9WK23 GO:0005975,GO:0043169,GO:0005737,GO:0004134 2.4.1.25 P9WK23 NC_000962.3 Mycobrowser_v5 CDS 2028425 2029477 . + 0 Rv1790 PPE27 Function unknown PPE family protein PPE27 Rv1790, (MTV049.12), len: 350 aa. PPE27, Member of the Mycobacterium tuberculosis PPE family of glycine-rich protein, similar to Z74024|MTCY274.24 Mycobacterium tuberculosis cosmid (404 aa), FASTA scores: opt: 849, E(): 0, (50.0% identity in 406 aa overlap). Q79FK5 No PE/PPE Q79FK5 Q79FK5 Q79FK5 NC_000962.3 Mycobrowser_v5 CDS 3709715 3710248 . - 0 Rv3324c moaC3 Thought to be involved in the biosynthesis of molybdopterin. Probable molybdenum cofactor biosynthesis protein C 3 MoaC3 Rv3324c, (MTV016.24c), len: 177 aa. Probable moaC3, molybdopterin cofactor biosynthesis protein, highly similar to others e.g. Q9HX95|MOAC|PA3918 from Pseudomonas aeruginosa (160 aa), FASTA scores: opt: 567, E(): 7.5e-30, (58.35% identity in 156 aa overlap); Q9RKA8|MOAC from Streptomyces coelicolor (170 aa), FASTA scores: opt: 553, E(): 6.3e-29, (58.25% identity in 158 aa overlap); P30747|MOAC_ECOLI|CHLA3|B0783 from Escherichia coli strain K12 (160 aa), FASTA scores: opt: 516, E(): 1.5e-26, (55.95% identity in 159 aa overlap); etc. Also highly similar to O05788|MOAC1|Rv3111|MTCY164.21 putative molybdenum cofactor biosynthesis protein C from Mycobacterium tuberculosis (170 aa), FASTA scores: opt: 734, E(): 1.3e-40, (71.8% identity in 163 aa overlap); and Rv0864|MOAC2|MTV043.57 putative molybdenum cofactor biosynthesis protein (167 aa). P9WJR5 No intermediary metabolism and respiration P65392 P9WJR5 GO:0006777 P9WJR5 NC_000962.3 Mycobrowser_v5 CDS 2031066 2031968 . + 0 Rv1794 Rv1794 Function unknown Conserved protein Rv1794, (MTV049.16), len: 300 aa. Conserved protein, slight similarity to Mycobacterium tuberculosis O53694|Rv0289|MTV035.17, (295 aa), FASTA scores: opt: 172, E(): 0.00083, (25.7% identity in 261 aa overlap). Equivalent to Mycobacterium leprae hypothetical protein Q9Z5I1|MLCB596.31|AL035472 (300 aa), (88.0% identity in 300 aa overlap). Contains PS00211 ABC transporters family signature. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53943 No conserved hypotheticals O53943 O53943 NC_000962.3 Mycobrowser_v5 CDS 2033729 2035486 . + 0 Rv1796 mycP5 Thought to have proteolytic activity. Probable proline rich membrane-anchored mycosin MycP5 (serine protease) (subtilisin-like protease) (subtilase-like) (mycosin-5) Rv1796, (MTV049.18), len: 585 aa. Probable mycP5, pro-rich membrane-anchored serine protease (mycosin) (see citations below). Member of family with four other Mycobacterium tuberculosis serine proteases: Rv3886c|O05458|MTCY15F10.26|Z94121 (550 aa), FASTA scores: opt: 1173, E(): 0, (47.9% identity in 578 aa overlap); Rv0291, Rv3883c, and Rv3449. Genes all linked to those of ESAT-6 family. Has possible N-terminal signal peptide and hydrophobic anchor-like stretch at C-terminus. Contains two serine protease, subtilase family active site motifs: a aspartic acid active site motif (PS00136); and a histidine active site motif (PS00137). Belongs to peptidase family S8 (also known as the subtilase family), pyrolysin subfamily. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). O53945 No intermediary metabolism and respiration O53945 O53945 GO:0004252,GO:0006508 3.4.21.- O53945 NC_000962.3 Mycobrowser_v5 CDS 2035483 2036703 . + 0 Rv1797 eccE5 Function unknown ESX conserved component EccE5. ESX-5 type VII secretion system protein. Probable membrane protein. Rv1797, (MTV049.19), len: 406 aa. eccE5, esx conserved component, ESX-5 type VII secretion system protein, probable membrane protein, some similarity to Mycobacterium tuberculosis O05462|Rv3882c|MTCY15F10.30|Z94121 (462 aa), FASTA scores: opt: 181, E(): 9.2e-05, (25.4% identity in 283 aa overlap). Has two hydrophobic stretch near N-terminus. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WJE3 No cell wall and cell processes O53946 P9WJE3 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2036700 2038532 . + 0 Rv1798 eccA5 Function unknown ESX conserved component EccA5. ESX-5 type VII secretion system protein. Rv1798, (MTV049.20), len: 610 aa. eccA5, esx conserved component, ESX-5 type VII secretion system protein, similar to several mycobacterial proteins e.g. O05460|MTCY15F10.28|Rv3884c|Z94121 from M. tuberculosis (619 aa), FASTA scores: opt: 669, E(): 0, (31.0% identity in 549 aa overlap); and O33089|MLCB628.18c|Y14967 from Mycobacterium leprae (573 aa), FASTA scores: opt: 723, E(): 0, (32.4% identity in 568 aa overlap). Also very similar to Rv0282. May belong to the CbxX/CfqX family as last ~320 aa domain very similar to several family members. Contains ATP/GTP-binding site motif A (P-loop; PS00017). P9WPI1 No cell wall and cell processes P63744 P9WPI1 GO:0005737,GO:0017111,GO:0005524 P9WPI1 NC_000962.3 Mycobrowser_v5 CDS 2044923 2046842 . - 0 Rv1803c PE_PGRS32 Function unknown PE-PGRS family protein PE_PGRS32 Rv1803c, (MTV049.25c), len: 639 aa. PE_PGRS32, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below). Most similar to Rv1768|MTCY28.34|Z95890 (618 aa), FASTA scores: opt: 1827, E(): 0, (53.5% identity in 664 aa overlap). Contains two PS00583 pfkB family of carbohydrate kinases signatures 1. Predicted to be an outer membrane protein (See Song et al., 2008). Q79FJ9 No PE/PPE Q79FJ9 Q79FJ9 NC_000962.3 Mycobrowser_v5 CDS 2051282 2052688 . + 0 Rv1809 PPE33 Function unknown PPE family protein PPE33 Rv1809, (MTV049.31), len: 468 aa. PPE33, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, most similar to RV1802AL022021|MTV049.23 (463 aa), FASTA scores: opt: 1238, E(): 0, (51.2% identity in 471 aa overlap). Alternative nucleotide at position 2051746 (T->C; A155A) has been observed. P9WI03 No PE/PPE Q79FJ5 P9WI03 P9WI03 NC_000962.3 Mycobrowser_v5 CDS 2056521 2057423 . + 0 Rv1814 erg3 Involved in lipid desaturation Membrane-bound C-5 sterol desaturase Erg3 (sterol-C5-desaturase) Rv1814, (MTCY1A11.29c), len: 300 aa. Erg3, transmembrane C-5 sterol desaturase (see *), weak similarity to several e.g. ERG3_YEAST|P32353 c-5 sterol desaturase (365 aa), FASTA scores: opt: 154, E(): 0.0011, (22.9% identity in 288 aa overlap). Belongs to the sterol desaturase family. [* Note: work of Jackson, C.J., Lamb, D.C., Kelly, D.E., Kelly, S.L., Characterization of a sterol delta 5,6-desaturase homolog in Mycobacterium bovis (BCG). Submitted (jun-2000) to the EMBL/GenBank/DDBJ databases]. P9WNZ9 No lipid metabolism P68435 P9WNZ9 GO:0006633,GO:0016021,GO:0005506,GO:0055114,GO:0016491,GO:0005886,GO:0016126,GO:0005783 1.3.-.- NC_000962.3 Mycobrowser_v5 CDS 2062809 2064728 . - 0 Rv1819c bacA Thought to be involved in active transport of drugs across the membrane (export): multidrugs resistance by an export mechanism. Responsible for energy coupling to the transport system and for the translocation of the substrate across the membrane. Probable drug-transport transmembrane ATP-binding protein ABC transporter BacA Rv1819c, (MTCY1A11.24), len: 639 aa. Probable bacA, drug-transport transmembrane ATP-binding protein ABC transporter (see citation below), equivalent to AL008609|MLCB1788.47 hypothetical ABC transporter from Mycobacterium leprae (638 aa), (74.9% identity in 634 aa overlap). Also similar to other transmembrane ATP-binding proteins e.g. Q57335|Y036_HAEIN hypothetical ABC transporter ATP-binding protein from Haemophilus influenzae (592 aa), FASTA scores: opt: 1235, E(): 2.8e-61, (40.8% identity in 623 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). P9WQI9 No cell wall and cell processes Q50614 P9WQI9 GO:0042626,GO:0016021,GO:0005886,GO:0055085,GO:0005524 P9WQI9 NC_000962.3 Mycobrowser_v5 CDS 3710433 3710759 . + 0 Rv3325 Rv3325 Involved in the transposition of the insertion sequence element IS6110. Probable transposase for insertion sequence element IS6110 (fragment) Rv3325, (MTV016.25), len: 108 aa. Putative Transposase for IS6110 (fragment). Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv3325 and Rv3326, the sequence UUUUAAAG (directly upstream of Rv3326) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Belongs to the transposase family 8. P9WKH5 No insertion seqs and phages P0C5G9 P9WKH5 GO:0004803,GO:0006313,GO:0003677 P9WKH5 NC_000962.3 Mycobrowser_v5 CDS 2066457 2068883 . + 0 Rv1821 secA2 Involved in protein export. May interact with the SECY/SECE subunits. SECA has a central role in coupling the hydrolysis of ATP to the transfer of PRE-secretory periplasmic and outer membrane proteins across the membrane. Possible preprotein translocase ATPase SecA2 Rv1821, (MTCY1A11.22c), len: 808 aa. Possible secA2, preprotein translocase and ATPase, component of secretion apparatus (see Braunstein & Belisle 2000), similar to several preprotein translocases e.g. P28366|SECA_BACSU preprotein translocase secA subunit from Bacillus subtilis (841 aa), FASTA scores: opt: 1424, E(): 0, (35.9% identity in 786 aa overlap). Equivalent to AL008609|MLCB1788.45 Preprotein translocase SecA 2 from Mycobacterium leprae (778 aa) (87.1% identity in 780 aa overlap). Also similar to Rv3240c|MTCY20B11.15c secA preprotein translocase from Mycobacterium tuberculosis (949 aa). Could be part of the prokaryotic protein translocation apparatus which comprise SECA|Rv3240c, SECD|Rv2587c, SECE|Rv0638, SECF|Rv2586c, SECG|Rv1440 and SECY|Rv0732. Binds ATP. P9WGP3 No cell wall and cell processes P66785 P9WGP3 GO:0005737,GO:0065002,GO:0005886,GO:0005515,GO:0017038,GO:0006605,GO:0005524 P9WGP3 NC_000962.3 Mycobrowser_v5 CDS 2071952 2072356 . + 0 Rv1826 gcvH The glycine cleavage system catalyses the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein Probable glycine cleavage system H protein GcvH Rv1826, (MTCY1A11.17c), len: 134 aa. Probable gcvH, glycine cleavage system H protein, highly similar to GCSH_ECOLI|P23884 glycine cleavage system H protein from Escherichia coli (129 aa), FASTA scores: opt: 428, E(): 2.2e-22, (47.8% identity in 134 aa overlap). Equivalent to MLCB1788.37c gcvH from Mycobacterium leprae (78.4% identity in 134 aa overlap). Contains PS00189 2-oxo acid dehydrogenases acyltransferase component lipoyl binding site. Belongs to the GcvH family. P9WN55 No intermediary metabolism and respiration 3HGB,3IFT Q50607 P9WN55 GO:0019464,GO:0031405,GO:0005739,GO:0005960 P9WN55 NC_000962.3 Mycobrowser_v5 CDS 2075877 2078702 . + 0 Rv1832 gcvB The glycine cleavage system catalyses the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein [catalytic activity: glycine + lipoylprotein = S- aminomethyldihydrolipoylprotein + CO(2)] Probable glycine dehydrogenase GcvB (glycine decarboxylase) (glycine cleavage system P-protein) Rv1832, (MTCY1A11.11c), len: 941 aa. Probable gcvB, glycine dehydrogenase [decarboxylating], highly similar to GCSP_ECOLI|P33195 glycine dehydrogenase (decarboxylating) from Escherichia coli (957 aa), FASTA scores: opt: 2194, E(): 0, (55.4% identity in 961 aa overlap). The glycine cleavage system is composed of four proteins: P, T, L, and H P9WN53 No intermediary metabolism and respiration Q50601 P9WN53 GO:0004375,GO:0055114,GO:0030170,GO:0019464 1.4.4.2 P9WN53 NC_000962.3 Mycobrowser_v5 CDS 2082603 2084636 . - 0 Rv1836c Rv1836c Function unknown Conserved protein Rv1836c, (MTCY1A11.07), len: 677 aa. Conserved protein. Equivalent to MLCB1788.28|AL008609 hypothetical protein from Mycobacterium leprae (710 aa), FASTA scores: opt: 2938, E(): 0, (66.0% identity in 714 aa overlap). Contains PS00036 bZIP transcription factors basic domain signature. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WLQ9 No conserved hypotheticals P9WLQ9 GO:0005886,GO:0016021 P9WLQ9 NC_000962.3 Mycobrowser_v5 CDS 2087971 2089518 . - 0 Rv1840c PE_PGRS34 Function unknown PE-PGRS family protein PE_PGRS34 Rv1840c, (MTCY359.33), len: 515 aa. PE_PGRS34, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see citation below). Similar to many e.g. Y03A_MYCTU|Q10637 hypothetical glycine-rich 49.6 kDa protein (603 aa), FASTA scores: opt: 1693, E(): 0, (53.1% identity in 612 aa overlap); etc. P9WIF3 No PE/PPE Q50594 P9WIF3 GO:0005886,GO:0016021 P9WIF3 NC_000962.3 Mycobrowser_v5 CDS 2090718 2092085 . - 0 Rv1842c Rv1842c Unknown Conserved hypothetical membrane protein Rv1842c, (MTCY359.31), len: 455 aa. Conserved hypothetical membrane protein. Similar to Z99109|0O7589 Potential integral membrane protein from Bacillus subtilis (461 aa), FASTA scores: opt: 723, E(): 0, (31.2% identity in 449 aa overlap). Similar to other Mycobacterium tuberculosis putative integral membrane proteins e.g. Rv2366c, Rv1841c. P9WFP3 No cell wall and cell processes Q50592 P9WFP3 GO:0005886,GO:0016021 P9WFP3 NC_000962.3 Mycobrowser_v5 CDS 2093731 2095188 . - 0 Rv1844c gnd1 Involved in hexose monophosphate shunt (pentose phosphate pathway) [catalytic activity: 6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH]. Probable 6-phosphogluconate dehydrogenase Gnd1 Rv1844c, (MTCY359.29), len: 485 aa. Probable gnd1, 6-phosphogluconate dehydrogenase. Similar to others e.g. 6PGD_ECOLI|P00350 from Escherichia coli (468 aa), FASTA scores: opt: 1661, E(): 0, (53.6% identity in 466 aa overlap); etc. Also similar to Rv1122|MTCY22G8.11|gnd2 probable 6-phosphogluconate dehydrogenase, decarboxylating from Mycobacterium tuberculosis (340 aa), FASTA score: (33.0% identity in 351 aa overlap). Note that Rv1844c is most similar to gnd's from Gram negative organisms, while Rv1122|MTCY22G8.11|gnd2 is most similar to gnd's from Gram positive organisms. Belongs to the 6-phosphogluconate dehydrogenase family. Q79FJ2 No intermediary metabolism and respiration Q79FJ2 Q79FJ2 GO:0055114,GO:0006098,GO:0004616,GO:0050661 1.1.1.44 Q79FJ2 NC_000962.3 Mycobrowser_v5 CDS 2097961 2099694 . + 0 Rv1850 ureC Involved in the conversion of urea to NH3 [catalytic activity: urea + H2O = CO2 + 2 NH3] Urease alpha subunit UreC (urea amidohydrolase) Rv1850, (MTCY359.23c), len: 577 aa. UreC, urease alpha subunit. Similar to URE1_MYCTU|P50042 from Mycobacterium tuberculosis (577 aa), FASTA scores: opt: 3794, E(): 0, (98.3% identity in 577 aa overlap). Contains PS00145 Urease active site motif. Belongs to the urease family. P9WFF1 No intermediary metabolism and respiration P0A660 P9WFF1 GO:0016151,GO:0019627,GO:0009039,GO:0005737 3.5.1.5 P9WFF1 NC_000962.3 Mycobrowser_v5 CDS 2101651 2103042 . - 0 Rv1854c ndh Transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Does not couple the redox reaction to proton translocation. Probable NADH dehydrogenase Ndh Rv1854c, (MTCY359.19), len: 463 aa. Probable ndh, NADH dehydrogenase (see citations below), similar to several e.g. S74826 NADH dehydrogenase from Synechocystis sp. (445 aa), FASTA score: opt: 1228, E(): 0, (46.3% identity in 432 aa overlap). Highly similar to Rv0392c|Z84725|g1817703 from Mycobacterium tuberculosis (470 aa), FASTA scores: opt: 1911, E(): 0, (64.7% identity in 459 aa overlap); and Rv1812c. P95160 No intermediary metabolism and respiration P95160 P95160 GO:0003954,GO:0055114,GO:0050660 1.6.99.3 INH P95160 NC_000962.3 Mycobrowser_v5 CDS 2105773 2106567 . + 0 Rv1858 modB Part of the binding-protein-dependent transport system modabc for molybdenum; responsible for the translocation of the substrate across the membrane. Probable molybdenum-transport integral membrane protein ABC transporter ModB Rv1858, (MTCY359.15c), len: 264 aa. Probable modB, molybdenum-transport integral membrane protein ABC transporter (see citation below), similar to others e.g. Y10817|ANY108175 ModB from Arthrobacter (239 aa), FASTA scores: opt: 937, E(): 0, (67.8% identity in 230 aa overlap); etc. Similar to other Mycobacterium tuberculosis transport proteins e.g. Rv2039c, Rv2316, etc. P9WG13 No cell wall and cell processes P0A624 P9WG13 GO:0015098,GO:0015689,GO:0005886,GO:0016021 P9WG13 NC_000962.3 Mycobrowser_v5 CDS 2106574 2107683 . + 0 Rv1859 modC Part of the binding-protein-dependent transport system modabc for molybdenum; responsible for energy coupling to the transport system. Probable molybdenum-transport ATP-binding protein ABC transporter ModC Rv1859, (MTCY359.14c), len: 369 aa. Probable modC, molybdenum-transport ATP-binding protein ABC transporter (see citation below), similar to others e.g. Y10817|ANY108176 ModC from Arthrobacter (349 aa), FASTA scores: opt: 895, E(): 0, (46.0% identity in 361 aa overlap); etc. Shows similarity to other Mycobacterium tuberculosis ABC-transporter proteins e.g. Rv0073, Rv1238, Rv2564, etc. Contains both PS00017 ATP/GTP-binding site motif A (P-loop) and PS00211 ABC transporters family signatures involved in molybdate uptake. Belongs to the ATP-binding transport protein family (ABC transporters). P9WQL3 No cell wall and cell processes P95155 P9WQL3 GO:0043190,GO:0030001,GO:0015689,GO:0015412,GO:0042888,GO:0005886,GO:0005524 3.6.3.29 P9WQL3 NC_000962.3 Mycobrowser_v5 CDS 3796448 3797437 . - 0 Rv3382c lytB1 Unknown. Possibly involved in drug/antibiotic tolerance. In other organisms, LYTB product is involved in penicillin tolerance and control of the stringent response. Probable LYTB-related protein LytB1 Rv3382c, (MTV004.40c), len: 329 aa. Probable lytB1, lytB-related protein, highly similar to many e.g. Q9HVM7|LYTB_PSEAE|PA4557 from Pseudomonas aeruginosa (314 aa), FASTA scores: opt: 1048, E(): 2e-55, (53.2% identity in 314 aa overlap); Q9JR39|LYTB|NMA0624|NMB1831 from Neisseria meningitidis (serogroup a and B) (322 aa), FASTA scores: opt: 1041, E(): 5.4e-55, (52.25% identity in 312 aa overlap); P22565|LYTB_ECOLI|B0029 from Escherichia coli strain K12 (316 aa), FASTA scores: opt: 1013, E(): 2.5e-53, (51.45% identity in 311 aa overlap) (for more information about lytB protein, see citation below); Q9X781|LYTB_MYCLE|LYTB2|ML1938|MLCB1222.06c from Mycobacterium leprae (332 aa), FASTA scores: opt: 979, E(): 2.8e-51, (51.3% identity in 312 aa overlap); etc. Also similar to Q9PAS9|XF2416 drug tolerance protein from Xylella fastidiosa (316 aa), FASTA scores: opt: 1043, E(): 4.1e-55, (53.65% identity in 315 aa overlap). And similar to O53458|Rv1110|LYTB2|MTV017.63 from Mycobacterium tuberculosis (335 aa), FASTA scores: opt: 975, E(): 4.9e-51, (51.3% identity in 312 aa overlap). Belongs to the LytB family. P9WKF9 No cell wall and cell processes P0A5I2 P9WKF9 GO:0051745,GO:0019288,GO:0046872,GO:0055114,GO:0016114,GO:0051538 1.17.1.2 P9WKF9 NC_000962.3 Mycobrowser_v5 CDS 2113140 2115476 . + 0 Rv1866 Rv1866 Function unknown, but supposed involvement in lipid degradation. Conserved protein Rv1866, (MTCY359.07c), len: 778 aa. Conserved protein, N-terminal region similar to fatty acyl-CoA racemases e.g. Rv0855, Rv1143, and C-terminal region (from aa 370) similar to L-carnitine dehydratases, racemases, and Rv3272|MTCY71.12 Mycobacterium tuberculosis (394 aa), FASTA score: opt: 472, E(): 2.1e-21, (29.9% identity in 388 aa overlap). Also similar to P31572|CAIB_ECOLI L-carnitine dehydratase (405 aa), FASTA score: opt: 306, E(): 2.1e-11, (23.3% identity in 424 aa overlap). P95149 No lipid metabolism P95149 P95149 GO:0008152,GO:0003824 P95149 NC_000962.3 Mycobrowser_v5 CDS 2115764 2117248 . + 0 Rv1867 Rv1867 Function unknown, but supposed involvement in lipid degradation. Conserved protein Rv1867, (MTCY359.06c), len: 494 aa. Conserved protein, some similarity to acetyl CoA synthase and to lipid carriers. FASTA best: E155295 acetyl CoA synthase (388 aa), opt: 213, E(): 4.5e-07, (23.2% identity in 423 aa overlap), P95148 No lipid metabolism P95148 GO:0008152,GO:0003824 P95148 NC_000962.3 Mycobrowser_v5 CDS 2121907 2123151 . - 0 Rv1872c lldD2 Involved in respiration; catalyzes conversion of lactate into pyruvate [catalytic activity: (S)-lactate + 2 ferricytochrome C = pyruvate + 2 ferrocytochrome C]. Possible L-lactate dehydrogenase (cytochrome) LldD2 Rv1872c, (MTCY180.46, MTCY359.01), len: 414 aa (start uncertain). Possible lldD2, L-lactate dehydrogenase (cytochrome), similar to other lactate dehydrogenases and other oxidases e.g. LLDD_ECOLI|P33232 l-lactate dehydrogenase (cytochrome) from Escherichia coli strain K12 (396 aa), FASTA results: opt: 674, E(): 1.1e-37, (40.5% identity in 279 aa overlap); Q51135 lactate dehydrogenase from Neisseria meningitidis (390 aa), FASTA results: opt: 309, E(): 4.1e-15, (42.5% identity in 113 aa overlap); etc. Also shows similarity with Rv0694|lldD1|MTCY210.11 possible L-lactate dehydrogenase (cytochrome) from Mycobacterium tuberculosis (396 aa). Contains PS00557 FMN-dependent alpha-hydroxy acid dehydrogenases active site. Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenases family. Phosphorylated in vitro by PknJ|Rv2088 (See Arora et al., 2010). P9WND5 No intermediary metabolism and respiration P95143 P9WND5 GO:0004460,GO:0055114,GO:0010181 1.1.2.3 P9WND5 NC_000962.3 Mycobrowser_v5 CDS 2179673 2180446 . + 0 Rv1927 Rv1927 Function unknown Conserved hypothetical protein Rv1927, (MTCY09F9.37c), len: 257 aa. Conserved hypothetical protein, similar to SCG11A.10c|AL133210 hypothetical protein from Streptomyces coelicolor (252 aa), FASTA scores: opt: 729, E(): 0, (48.3% identity in 238 aa overlap). Slight similarity with P54543|YQJF_BACSU hypothetical 23.9 kDa protein from Bacillus subtilis (209 aa), FASTA scores, opt: 230, E(): 2.8e-08, (28.0% identity in 164 aa overlap). P95287 No conserved hypotheticals P95287 P95287 P95287 NC_000962.3 Mycobrowser_v5 CDS 2125340 2125819 . + 0 Rv1876 bfrA Involved in iron storage (may perform analogous functions in iron detoxification and storage as that of animal ferritins); ferritin is an intracellular molecule that stores iron in a soluble, nontoxic, readily available form. The functional molecule, which is composed of 24 chains, is roughly spherical and contains a central cavity in which the polymeric ferric iron core is deposited. Probable bacterioferritin BfrA Rv1876, (MTCY180.42c), len: 159 aa. Probable bfrA (alternate gene name: bfr), bacterioferritin (see citation below), similar to BFR_MYCLE|P43315 bacterioferritin (bfr) from Mycobacterium leprae (159 aa), FASTA results: opt: 958, E(): 0, (90.6% identity in 159 aa overlap). Also similar to Rv3841|MTCY01A6.28c|bfrB possible bacterioferritin from Mycobacterium tuberculosis (181 aa). Belongs to the bacterioferritin family. P9WPQ9 No intermediary metabolism and respiration 2WTL P63697 P9WPQ9 GO:0008199,GO:0006826,GO:0055114,GO:0016491,GO:0006879 P9WPQ9 NC_000962.3 Mycobrowser_v5 CDS 2125904 2127967 . + 0 Rv1877 Rv1877 Unknown; possibly involved in transport of drug across the membrane. Probable conserved integral membrane protein Rv1877, (MTCY180.41c), len: 687 aa. Probable conserved integral membrane protein, part of major facilitator superfamily (MFS), similar to many antibiotic and drug efflux proteins. Similar to e.g. Q56175 TU22 dTDP-glucose dehydrtatase from Streptomyces violaceoruber (557 aa), FASTA scores: opt: 895, E(): 0, (34.7% identity in 528 aa overlap). Also similar to Mycobacterium tuberculosis relatives protein, include Rv3728, Rv3239c, Rv2846c, etc. Contains PS00217 Sugar transport proteins signature 2 (PS00217). P9WG85 No cell wall and cell processes O07753 P9WG85 GO:0005886,GO:0046677,GO:0015904,GO:0015520,GO:0055085,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2129377 2130513 . + 0 Rv1879 Rv1879 Function unknown Conserved hypothetical protein Rv1879, (MTCY180.39c), len: 378 aa. Conserved hypothetical protein, similar to SCC22.14c|AL096839 hypothetical protein from Streptomyces coelicolor (368 aa), FASTA results: opt: 772, E(): 0 (40.3% identity in 372 aa overlap); and to N-terminal half of nodulin/glutamate-ammonia ligase-like protein. Some similarity to N-terminus of AL132958|ATT4D2_11 Arabidopsis thaliana (845 aa), FASTA results: opt: 354, E(): 3.1e-16, (29.2% identity in 383 aa overlap); and to P38094|FLUG_EMENI Flug protein of Emericella nidulans (865 aa), FASTA results: opt: 306, E(): 6.2e-13, (26.5% identity in 415 aa overlap). Note that the upstream ORF Rv1878|MTCY18 0.40c is similar to the C-terminus. O07751 No conserved hypotheticals O07751 O07751 GO:0008152,GO:0003824 O07751 NC_000962.3 Mycobrowser_v5 CDS 2130541 2131857 . - 0 Rv1880c cyp140 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Probable cytochrome P450 140 Cyp140 Rv1880c, (MT1929, MTCY180.38), len: 438 aa. Probable cyp140, cytochrome p450. Similar to Q00441|CPXJ_SACER 6-deoxyerythronolide beta hydroxylase (404 aa), FASTA scores: opt: 775, E(): 0, (44.2% identity in 319 aa overlap); and other members of the cytochrome P450 family. Related to Mycobacterium tuberculosis proteins include: Rv0766c, Rv2266, Rv0778, etc. Contains cytochrome P450 cysteine heme-iron ligand signature (PS00086). Belongs to the cytochrome P450 family. P9WPL9 No intermediary metabolism and respiration P63721 P9WPL9 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPL9 NC_000962.3 Mycobrowser_v5 CDS 2132370 2133203 . - 0 Rv1882c Rv1882c Function unknown; probably involved in cellular metabolism Probable short-chain type dehydrogenase/reductase Rv1882c, (MTCY180.36), len: 277 aa. Probable short-chain dehydrogenase/reductase, similar to various dehydrogenases/reductases, generally belonging to SDR family, e.g. NP_250789.1|NC_002516 probable short-chain dehydrogenase from Pseudomonas aeruginosa (251 aa); NP_421760.1|NC_002696 short chain dehydrogenase family protein from Caulobacter crescentus (270 aa); NP_107167.1|NC_002678 oxidoreductase (short chain dehydrogenase/reductase family) from Mesorhizobium loti (253 aa); P50197|LINC_PSEPA 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase from Pseudomonas paucimobilis (Sphingomonas paucimobilis) (250 aa), FASTA scores: opt: 301, E(): 2.3e-12, (30.0% identity in 223 aa overlap); etc. Also similar to proteins from Mycobacterium tuberculosis e.g. Rv3057c, Rv1245, etc. Contains possible helix-turn-helix motif at aa 246-267 (+4.32 SD). Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. O07749 No intermediary metabolism and respiration O07749 O07749 GO:0055114,GO:0016491 1.1.-.- O07749 NC_000962.3 Mycobrowser_v5 CDS 2134890 2135867 . - 0 Rv1886c fbpB Involved in cell wall mycoloylation. Proteins of the antigen 85 complex are responsible for the high affinity of mycobacteria to fibronectin. Possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity. Secreted antigen 85-B FbpB (85B) (antigen 85 complex B) (mycolyl transferase 85B) (fibronectin-binding protein B) (extracellular alpha-antigen) Rv1886c, (MT1934, MTCY180.32), len: 325 aa. FbpB (alternate gene names: mpt59, 85B), precursor of the 85-B antigen (fibronectin-binding protein B) (mycolyl transferase 85B) (see citations below), highly similar to other Mycobacterial antigen precursors e.g. P12942|A85B_MYCBO antigen 85-B precursor from Mycobacterium bovis (323 aa); P21160|A85B_MYCKA antigen 85-B precursor from Mycobacterium kansasii (325 aa); etc. Also highly similar to Mycobacterium tuberculosis antigen precursors: Rv3804c|fbpA (338 aa), Rv0129c|fbpC2 (340 aa), and Rv3803c|fbpC1 (299 aa). Predicted possible vaccine candidate (See Zvi et al., 2008). P9WQP1 No lipid metabolism 1F0N,1F0P P0C5B9 P9WQP1 GO:0005576,GO:0008415 2.3.1.- P9WQP1 NC_000962.3 Mycobrowser_v5 CDS 2194644 2194973 . - 0 Rv1942c mazF5 Function unknown Possible toxin MazF5 Rv1942c, (MTCY09F9.22), len: 109 aa. Possible mazF5, toxin, part of toxin-antitoxin (TA) operon with Rv1943c (See Pandey and Gerdes, 2005; Zhu et al., 2006), shows some similarity to Q10867|MTCY39.28|Rv1991 hypothetical 12.3 kDa protein (114 aa), FASTA scores: opt: 117, E(): 0.021, (24. 5% identity in 110 aa overlap) also P33645|CHPA_ECOLI pemk-like protein 1 (mazf protein) from Escherichia coli (111 aa), FASTA scores: opt: 104, E(): 0.18, (29.1% identity in 110 aa overlap). Also similar to Mycobacterium tuberculosis Rv0659c (102 aa) (32.7% identity in 101 aa overlap); Rv1102c (33.3% identity in 93 aa overlap) and Rv1495. P95272 No virulence, detoxification, adaptation P95272 P95272 GO:0003677 P95272 NC_000962.3 Mycobrowser_v5 CDS 2140739 2141869 . - 0 Rv1894c Rv1894c Function unknown Conserved hypothetical protein Rv1894c, (MTCY180.24), len: 376 aa. Conserved hypothetical protein, weak similarity to some oxidoreductases e.g. Q01284 2-nitropropane dioxygenase precursor (378 aa), FASTA results: opt: 204, E(): 5.8e-06, (34.3% identity in 140 aa overlap). Similar to hypothetical Mycobacterium tuberculosis proteins e.g. Rv3553|MTCY03C7.02c (355 aa), FASTA results: opt: 296, E(): 1.6e-10, (32.9% identity in 167 aa overlap); Rv1533 (375 aa) (48.1% identity in 376 aa overlap); Rv0021c, Rv2781c. O07738 No conserved hypotheticals O07738 O07738 GO:0055114,GO:0018580 O07738 NC_000962.3 Mycobrowser_v5 CDS 2146245 2147633 . - 0 Rv1900c lipJ Function unknown; probably involved in cellular metabolism Probable lignin peroxidase LipJ Rv1900c, (MTCY180.18), len: 462 aa. Probable lipJ, lignin peroxidase, with some similarity to esterases, hydrolases and hypothetical Mycobacterium tuberculosis proteins e.g. Q43936 beta-ketoadipate enol-lactone hydrolase from Acinetobacter calcoaceticus (267 aa), FASTA results: opt: 217, E(): 1.7e-07, (29.2% identity in 260 aa overlap). Also similar to other Mycobacterium tuberculosis hypothetical proteins e.g. Rv2212|Q10400|YM12_MYCTU (378 aa), FASTA results: opt: 216, E(): 6.7e-07, (27.7% identity in 285 aa overlap). O07732 No intermediary metabolism and respiration 1YBT,1YBU O07732 O07732 GO:0004601,GO:0016849,GO:0009190 O07732 NC_000962.3 Mycobrowser_v5 CDS 2153235 2153882 . - 0 Rv1907c Rv1907c Unknown Hypothetical protein Rv1907c, (MTCY180.11), len: 215 aa. Hypothetical unknown protein. Similar to Q50763 Ethyl methane sulphonate resistance protein from Mycobacterium tuberculosis (168 aa), FASTA scores: opt: 638, E(): 0, (69.7% identity in 152 aa overlap). Downstream of a cloned katG gene (EMBL:mtkatg). Differences are due to frameshift errors in the EMBL sequence and the use of an earlier start codon. Alternative nucleotide at position 2153410 (a->G; V158A) has been observed. L0TAY1 No conserved hypotheticals L0TAY1 NC_000962.3 Mycobrowser_v5 CDS 2153889 2156111 . - 0 Rv1908c katG Multifunctional enzyme, exhibiting both a catalase, a broad-spectrum peroxidase, and a peroxynitritase activities. May play a role in the intracellular survival of mycobacteria within macrophages; protection against reactive oxygen and nitrogen intermediates produced by phagocytic cells. Seems regulated by SIGB|Rv2710 [catalytic activity: 2 H(2)O(2) = O(2) + 2 H(2)O]. Catalase-peroxidase-peroxynitritase T KatG Rv1908c, (MTCY180.10), len: 740 aa. KatG, catalase-peroxidase-peroxynitritase T (see citations below), HPI. FASTA results: Q57215 catalase-peroxidase from Mycobacterium tuberculosis (740 aa) opt: 5081, E(): 0, (100% identity in 740 aa overlap). Contains peroxidases active site signature (PS00436) and ATP/GTP-binding site motif A (P-loop; PS00017). Cosmid sequence was corrected to agree with a sequencing read from the H37Rv genome. Deletions or defects in KATG gene cause isoniazid (INH) resistance. Belongs to the peroxidase family. Bacterial peroxidase/catalase subfamily. KATG transcription seems to be regulated by FURA|Rv1909c product. The catalase-peroxidase activity is associated with the amino-terminal domain but no definite function has been assigned to the carboxy-terminal domain. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WIE5 No virulence, detoxification, adaptation 1SFZ,1SJ2,2CCA,2CCD Q08129 P9WIE5 GO:0020037,GO:0042744,GO:0055114,GO:0046677,GO:0004096 1.11.1.7,1.11.1.6 INH P9WIE5 NC_000962.3 Mycobrowser_v5 CDS 2158087 2159091 . - 0 Rv1912c fadB5 Thought to be involved in fatty acid degradation. FADB and FADA are the alpha and beta subunits of the multifunctional enzyme complex of the fatty acid degradation cycle. Possible oxidoreductase FadB5 Rv1912c, (MTCY180.06), len: 334 aa. Possible fadB5, oxidoreductase, similar to various oxidoreductases: 3-hydroxyacyl-CoA dehydrogenase, quinone oxidoreductases, and polyketide synthases, e.g. NP_104067.1|NC_002678 probable oxidoreductase from Mesorhizobium loti (308 aa); NP_464140.1|NC_003210 protein similar to oxidoreductase from Listeria monocytogenes (313 aa); NP_193889.1|NC_003075 putative NADPH quinone oxidoreductase from Arabidopsis thaliana (325 aa); NP_001880.2|NM_001889 crystallin, zeta; quinone oxidoreductase; NADPH:quinone reductase from Homo sapiens (329 aa); part 2983 to 3197 of T17410 polyketide synthase type I from Streptomyces venezuelae (3739 aa); Q53927|SCBAC20F6.16 hydroxyacyl-CoA dehydrogenase from Streptomyces coelicolor (329 aa), FASTA scores: opt: 621, E(): 2e-30, (39.5% identity in 349 aa overlap); etc. Also similar to many hypothetical Mycobacterium tuberculosis proteins including: MTCY24G1.09, MTCY13D12.11, MTCY19H9.01, MTCY24G1.03, MTCY03A2.17c, etc. Contains quinone oxidoreductase/zeta-crystallin signature (PS01162). O07721 No lipid metabolism O07721 O07721 GO:0055114,GO:0008270,GO:0003960 1.6.5.5 O07721 NC_000962.3 Mycobrowser_v5 CDS 2161566 2162762 . + 0 Rv1916 aceAb Involved in glyoxylate bypass, an alternative to the tricarboxylic acid cycle [catalytic activity: isocitrate = succinate + glyoxylate]. Probable isocitrate lyase AceAb [second part] (isocitrase) (isocitratase) (Icl) Rv1916, (MTCY180.02c), len: 398 aa. Probable aceAb, isocitrate lyase (see citations below). Highly similar to the C-terminus of ACEA_MYCLE|P46831 isocitrate lyase from Mycobacterium leprae (606 aa), FASTA results: opt: 1635, E(): 0, (86.3% identity in 278 aa overlap). Although this ORF and the upstream ORF representing the N-terminal half of aceA could be joined by a frameshift no error is apparent in the cosmid, or in a seqencing read from the genome of H37Rv. As this ORF has a RBS and transcriptional start immediately following the stop of the upstream ORF, it is possible that they are expressed as two separate modules. In Mycobacterium tuberculosis strain CDC1551, aceA exists as a single gene, MT1966: the corresponding protein has been purified experimentally and seems have an active isocitrate lyase activity (see Honer et al., 1999). For Mycobacterium tuberculosis strain H37Rv, immunoblot assay didn't detect AceAa or AceAb products (see Honer et al., 1999) but mRNA of AceAa|Rv1915 has been detected (see Betts et al., 2002); so AceAb|Rv1916 could be a pseudogene. Icl2 has 2-methyl-isocitrate lyase (MCL) activity in M. tuberculosis Erdman (See Munoz-Elias et al., 2006). O07717 No intermediary metabolism and respiration O07717 O07717 GO:0004451,GO:0019752 4.1.3.1 O07717 NC_000962.3 Mycobrowser_v5 CDS 2171623 2172486 . + 0 Rv1920 Rv1920 Unknown Probable membrane protein Rv1920, (MTV050.04), len: 287 aa. Probable membrane protein, similar to AL0215|SC10A5.04 putative membrane protein from Streptomyces coelicolor cosmid 10A5 (295 aa), FASTA scores: opt: 292, E(): 3.6e-13, (31.3% identity in 243 aa overlap). Also weakly similar to several Mycobacterial putative proteins with unknown function e.g. Rv0502, Rv1428c, U00018_22 Mycobacterium leprae cosmid B2168. O53962 No cell wall and cell processes O53962 O53962 GO:0008152,GO:0008415 O53962 NC_000962.3 Mycobrowser_v5 CDS 2174067 2175182 . + 0 Rv1922 Rv1922 Unknown Probable conserved lipoprotein Rv1922, (MTCY09F9.42c), len: 371 aa. Probable conserved lipoprotein, possibly peptidase similar to many peptidases, e.g. P15555|DAC_STRSQ D-alanyl-D-alanine carboxypeptidase from Streptomyces sp. (406 aa), FASTA scores: opt: 382, E(): 3.1e-17, (28.0% identity in 379 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins Rv1497, Rv2463, Rv3775, etc. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site. P95291 No cell wall and cell processes P95291 P95291 P95291 NC_000962.3 Mycobrowser_v5 CDS 2177087 2178949 . + 0 Rv1925 fadD31 Function unknown, but involvement in lipid degradation. Probable acyl-CoA ligase FadD31 (acyl-CoA synthetase) (acyl-CoA synthase) Rv1925, (MTCY09F9.39c), len: 620 aa. Probable fadD31, acyl-CoA synthetase, highly similar to others from Mycobacterium leprae e.g. NP_301198.1|NC_002677 putative acyl-CoA synthetase (635 aa); NP_302537.1|NC_002677 probable acyl-CoA synthase (583 aa); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. fadD32 (637 aa); fadD21 (578 aa); fadD29 (619 aa); fadD26|FD26_MYCTU|Q10976 (626 aa), FASTA scores: opt: 945, E(): 0, (39.8% identity in 598 aa overlap); etc. Also similar to N-terminus of G1171128 saframycin MX1 synthetase B from Myxococcus xanthus (1770 aa), FASTA scores: opt: 845, E(): 0, (37.4% identity in 593 aa overlap); N-terminus of T34918 polyketide synthase from Streptomyces coelicolor (2297 aa); etc. Nucleotide position 2177654 in the genome sequence has been corrected, A:C resulting in M190L. I6Y7V6 No lipid metabolism P95288 I6Y7V6 GO:0008152,GO:0016874 6.2.1.- I6Y7V6 NC_000962.3 Mycobrowser_v5 CDS 2180450 2181217 . - 0 Rv1928c Rv1928c Function unknown; probably involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv1928c, (MTCY09F9.36), len: 255 aa. Probable short-chain dehydrogenase/reductase, highly similar to others e.g. NP_228109.1|NC_000853 oxidoreductase (short chain dehydrogenase/reductase family) from Thermotoga maritima (257 aa); T41116 short chain dehydrogenase from Schizosaccharomyces pombe (261 aa); P87219|SOU1_CANAL sorbitol utilization protein (SDR family) from Candida albicans (281 aa); P25529|HDHA_ECOLI 7-alpha-hydroxysteroid dehydrogenase from Escherichia coli (255 aa), FASTA scores: opt: 541, E(): 1.2e-27, (37.5% identity in 251 aa overlap); etc. Also similar to many mycobacterial tuberculosis proteins e.g. Rv1350, Rv0927c, Rv2002, Rv0769, Rv2766c, etc. Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. P95286 No intermediary metabolism and respiration P95286 P95286 GO:0055114,GO:0016491 1.-.-.- P95286 NC_000962.3 Mycobrowser_v5 CDS 2184959 2186188 . - 0 Rv1934c fadE17 Function unknown, but supposed involvement in lipid degradation. Probable acyl-CoA dehydrogenase FadE17 Rv1934c, (MTCY09F9.30), len: 409 aa. Probable fadE17, acyl-CoA dehydrogenase, highly similar to ACD_MYCLE|P46703 acyl-CoA dehydrogenase from Mycobacterium leprae (389 aa), FASTA scores: opt: 414, E(): 2.6e-19, (28.3% identity in 407 aa overlap). Also similar to many e.g. NP_249713.1|NC_002516 probable acyl-CoA dehydrogenase from Pseudomonas aeruginosa (381 aa); NP_420614.1|NC_002696 acyl-CoA dehydrogenase family protein from Caulobacter crescentus (355 aa); CAB61610.1|AL133210 putative acyl-CoA dehydrogenase from Streptomyces coelicolor (393 aa); etc. Also similar to others from Mycobacterium tuberculosis e.g. fadE30 (385 aa); fadE31 (377 aa); C-terminus of fadE34 (711 aa); etc. Could belong to the acyl-CoA dehydrogenases family. P95280 No lipid metabolism P95280 P95280 GO:0050660,GO:0003995,GO:0055114 1.3.99.- P95280 NC_000962.3 Mycobrowser_v5 CDS 2188496 2191015 . + 0 Rv1937 Rv1937 Function unknown; may be involved in electron transfer. Possible oxygenase Rv1937, (MTCY09F9.27c), len: 839 aa. Possible oxygenase, similar in N-terminus to N-terminal part (approx. 350 aa) of dioxygenases (including ring-hydroxylating dioxygenase electron transfer components) and monooxygenases, e.g. AAC34815.1|AF071556 anthranilate dioxygenase reductase from Acinetobacter sp. (343 aa); AAK52291.1|AY026914|AntC putative anthranilate dioxygenase reductase from Pseudomonas putida (340 aa); AAF63450.1|AF218267_7|AF218267 benzoate dioxygenase / ferredoxin reductase from Pseudomonas putida (336 aa); P23101|XYLZ_PSEPU toluate 1,2-dioxygenase electron transfer component [includes: ferredoxin; ferredoxin--NAD(+) reductase ] from Pseudomonas putida plasmid TOL pWW0 (336 aa), FASTA scores: opt: 700, E(): 0, (34.3% identity in 335 aa overlap); S23479 probable benzoate 1,2-dioxygenase reductase component benC from Acinetobacter calcoaceticus (338 aa); AAC45294.1|U81594 soluble methane monooxygenase protein C from Methylocystis sp. (343 aa); P22868|MEMC_METCA methane monooxygenase component C from Methylococcus capsulatus (348 aa); etc. Also similar in part to Mycobacterium tuberculosis hypothetical electron transfer proteins Rv3554, Rv3571, etc. Contains PS00197 2Fe-2S ferredoxins, iron-sulfur binding region signature. P95277 No intermediary metabolism and respiration P95277 P95277 GO:0009055,GO:0046872,GO:0055114,GO:0016491,GO:0051537 1.-.-.- P95277 NC_000962.3 Mycobrowser_v5 CDS 2192094 2192609 . + 0 Rv1939 Rv1939 Function unknown; probably involved in cellular metabolism Probable oxidoreductase Rv1939, (MTCY09F9.25c), len: 171 aa. Probable oxidoreductase, similar to NP_302637.1|NC_002677 probable oxidoreductase from Mycobacterium leprae (162 aa) Also similar to NTAB_CHELE|P54990 nitrilotriacetate monooxygenase component from Chelatobacter heintzii (322 aa), fasta scores: opt: 269, E(): 5.3e-11, (33.1% identity in 151 aa overlap). And similar to Mycobacterium tuberculosis probable monooxygenase components Rv0246, Rv3567, and to a lesser extent, Rv3007c. P95275 No intermediary metabolism and respiration P95275 P95275 GO:0055114,GO:0016491,GO:0010181 1.-.-.- P95275 NC_000962.3 Mycobrowser_v5 CDS 2202138 2202587 . + 0 Rv1956 higA Possibly involved in transcriptional mechanism. Possible antitoxin HigA Rv1956, (MTCY09F9.08c), len: 149 aa. Possible higA, antitoxin, part of toxin-antitoxin (TA) operon with Rv1955 (See Pandey and Gerdes, 2005; Gupta, 2009). Possible transcriptional regulatory protein, contains probable helix-turn-helix motif at aa 52-73 (+4.78 SD). Upon expression in E.coli Rv1956 has been shown to function as a toxin inhibiting cell growth and colony formation that is neutralized by coexpression with Rv1955 (PubMed: 19016878); It is not clear if these conflicting results are due to expression in a heterologous system. The gene names higA and higB have been assigned to both Rv1955 and Rv1956; we have chosen to call Rv1956 higA after consulting the authors. P9WJA7 No virulence, detoxification, adaptation P95258 P9WJA7 GO:0043565 P9WJA7 NC_000962.3 Mycobrowser_v5 CDS 2930344 2930784 . + 0 Rv2602 vapC41 Unknown Possible toxin VapC41. Contains PIN domain. Rv2602, (MTCI270A.03c), len: 146 aa. Possible vapC41, toxin, part of toxin-antitoxin (TA) operon with Rv2601A, contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis (strains H37Rv and CDC1551) e.g. O50457|Rv1242|MTV006.14 (143 aa), FASTA scores: opt: 147, E(): 0.0021, (26.25% identity in 141 aa overlap); P95023|Rv2530c|MTCY159.26 (139 aa), FASTA scores: opt: 131, E(): 0.027, (33.35% identity in 135 aa overlap); O53812|Rv0749|MTV041.23 (142 aa), FASTA scores: opt: 125, E(): 0.072, (26.45% identity in 140 aa overlap). P9WF59 No virulence, detoxification, adaptation O33215 P9WF59 P9WF59 NC_000962.3 Mycobrowser_v5 CDS 2205582 2206802 . - 0 Rv1963c mce3R Involved in transcriptional mechanism; repression of the MCE3 operon. Could also have a regulatory action on the MCE2 operon. Probable transcriptional repressor (probably TetR-family) Mce3R Rv1963c, (MTV051.01c-MTCY09F9.01), len: 406 aa. Probable mce3R, negative transcriptional regulatory protein, TetR family (see citation below); similar to several transcriptional regulator e.g. AL049485|SC6A5.30 Streptomyces coelicolor cosmid 6 a (404 aa), FASTA scores: opt: 319, E(): 6.4e-13, (29.5% identity in 373 aa overlap); and Z84498|MTCY9F9_1 (259 aa), FASTA scores: opt: 208, E(): 1.6e-07, (100.0% identity in 32 aa overlap). Contains probable helix-turn-helix at aa 36-57 (+4.23 SD) and two tet-R family signatures. P95251 No regulatory proteins P95251 P95251 GO:0006350,GO:0003700,GO:0006355 P95251 NC_000962.3 Mycobrowser_v5 CDS 2208507 2209322 . + 0 Rv1965 yrbE3B Unknown Conserved hypothetical integral membrane protein YrbE3B Rv1965, (MTV051.03), len: 271 aa. YrbE4B, hypothetical unknown integral membrane protein, part of mce3 operon and member of YrbE family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07413|Rv0168|MTCI28.08|yrbE1B (289 aa), FASTA scores: opt: 937, E(): 0, (54.3% identity in 254 aa overlap); O07790|Rv0588|MTCY19H5.34|yrbE2B (295 aa); etc. Also highly similar to conserved hypothetical integral membrane proteins of the yrbEB type, e.g. AAD24545.1|AF116213|YrbE1B from Mycobacterium leprae (106 aa); P45392|YRBE_ECOLI hypothetical 27.9 kDa protein from Escherichia coli (260 aa), FASTA scores: opt: 218, E(): 1.2e-07, (24.1% identity in 245 aa overlap); etc. The transcription of this CDS seems negatively regulated by the product of Rv1963c|mce3R (see Santangelo et al., 2002). O53966 No virulence, detoxification, adaptation O53966 O53966 O53966 NC_000962.3 Mycobrowser_v5 CDS 2209327 2210604 . + 0 Rv1966 mce3A Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce3A Rv1966, (MTV051.04), len: 425 aa. Mce3A; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins P72013|MCE1|Rv0169|MTCI28.09|mce1A (454 aa); O07789|MCE2|Rv0589|MTCY19H5.33c|mce2A (404 aa); etc. Also highly similar to others e.g. AAD52105.1|AF113402_1|AF113402 mycobacterial cell entry protein from Mycobacterium bovis BCG (454 aa); NP_302656.1|NC_002677 putative cell invasion protein from Mycobacterium leprae (441 aa); CAC12798.1|AL445327 putative secreted protein from Streptomyces coelicolor (418 aa); etc. Contains a possible N-terminal signal sequence or membrane anchor. Note that previously known as mce3. The transcription of this CDS seems negatively regulated by the product of Rv1963c|mce3R (see Santangelo et al., 2002). Predicted to be an outer membrane protein (See Song et al., 2008). L7N698 No virulence, detoxification, adaptation Q7D7P0 L7N698 L7N698 NC_000962.3 Mycobrowser_v5 CDS 2211626 2212858 . + 0 Rv1968 mce3C Unknown, but thought to be involved in host cell invasion. Mce-family protein Mce3C Rv1968, (MTV051.06), len: 410 aa. Mce3C; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07415|R0171|MTCI28.11|mce1C (515 aa); O07787|Rv0591|MTCY19H5.31|mce2C (481 aa); etc. Also similar to others e.g. CAC12796.1|AL445327 putative secreted protein from Streptomyces coelicolor (351 aa); NP_302658.1|NC_002677 putative secreted protein from Mycobacterium leprae (519 aa); etc. Contains a possible N-terminal signal sequence or membrane anchor. The transcription of this CDS seems negatively regulated by the product of Rv1963c|mce3R (see Santangelo et al., 2002). Predicted to be an outer membrane protein (See Song et al., 2008). O53969 No virulence, detoxification, adaptation O53969 O53969 O53969 NC_000962.3 Mycobrowser_v5 CDS 2219754 2220800 . + 0 Rv1977 Rv1977 Function unknown Conserved protein Rv1977, (MTV051.15), len: 348 aa. Conserved protein, similar to SCC123.20|AL136518 hypothetical protein from Streptomyces coelicolor (402 aa), blastp scores: Score = 311 bits (789), Expect = 5e-84 Identities = 156/316 (49%), Positives = 212/316 (66%); and PCC6803|D90907_31 Synechocystis sp. (303 aa), FASTA scores: opt: 533, E(): 4.7e- 29, (38.5% identity in 275 aa overlap). Contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. Alternative nucleotide at position 2219929 (T->C; L59P) has been observed. O53978 No conserved hypotheticals O53978 O53978 GO:0046872,GO:0004222,GO:0006508,GO:0016021 O53978 NC_000962.3 Mycobrowser_v5 CDS 2234991 2237306 . - 0 Rv1992c ctpG Metal cation-transporting ATPase; possibly catalyzes the transport of an undetermined metal cation with the hydrolysis of ATP [catalytic activity: ATP + H(2)O + undetermined metal cation(in) = ADP + phosphate + undetermined metal cation(out)]. Probable metal cation transporter P-type ATPase G CtpG Rv1992c, (MTCY39.27), len: 771 aa. Probable ctpG, metal cation-transporting P-type ATPase G (transmembrane protein), similar to others, especially cadmium-transporting ATPases, e.g. NP_244904.1|NC_002570 cadmium-transporting ATPase from Bacillus halodurans (707 aa); P30336|CADA_BACFI probable cadmium-transporting ATPase from Bacillus firmus (723 aa); BAB47609.1|AB037671 cadmium resistance protein B from Staphylococcus aureus (804 aa); 3121832|Q60048|CADA_LISMO probable cadmium-transporting ATPase from Listeria monocytogenes (707 aa); etc. Also similar to others from Mycobacterium tuberculosis e.g. Rv0969|MTCY10D7.05c|ctpV putative cation transporter P-type ATPase V (770 aa); Rv1469; Rv0092; etc. Contains PS00435 Peroxidases proximal heme-ligand signature and PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases), subfamily IB. P9WPS7 No cell wall and cell processes P63689 P9WPS7 GO:0006754,GO:0015662,GO:0016021,GO:0046872,GO:0046873,GO:0030001,GO:0005886,GO:0005524 3.6.3.- P9WPS7 NC_000962.3 Mycobrowser_v5 CDS 2224220 2225188 . - 0 Rv1981c nrdF1 Involved in the DNA replication pathway. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides, precursors that are necessary for DNA synthesis [catalytic activity: 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H(2)O = ribonucleoside diphosphate + reduced thioredoxin]. Ribonucleoside-diphosphate reductase (beta chain) NrdF1 (ribonucleotide reductase small subunit) (R2F protein) Rv1981c, (MTCY39.38), len: 322 aa. NrdF1, ribonucleoside-diphosphate reductase, beta chain (see citation below), highly similar to others e.g. RIR4_SALTY|P17424 ribonucleoside-diphosphate reductase (319 aa), FASTA scores: opt: 1402, E(): 0, (66.0% identity in 315 aa overlap); etc. Also similar to Rv3048c|MTV012.63c from Mycobacterium tuberculosis. Contains PS00368 Ribonucleotide reductase small subunit signature. Belongs to the ribonucleoside diphosphate reductase small chain family. Cofactor: binds 2 iron ions (by similarity). Note that previously known as nrdF. P9WH73 No information pathways Q10840 P9WH73 GO:0009186,GO:0055114,GO:0004748,GO:0046914,GO:0006260 1.17.4.1 P9WH73 NC_000962.3 Mycobrowser_v5 CDS 2226244 2227920 . + 0 Rv1983 PE_PGRS35 Function unknown PE-PGRS family protein PE_PGRS35 Rv1983, (MTCY39.36c), len: 558 aa. PE_PGRS35, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002). Similar to other PE proteins e.g. Rv0977, etc. Contains PS00141 Eukaryotic and viral aspartyl proteases active site. P9WIF1 No PE/PPE Q10873 P9WIF1 GO:0005886,GO:0016021 P9WIF1 NC_000962.3 Mycobrowser_v5 CDS 2243816 2245138 . - 0 Rv1999c Rv1999c Unknown. Possibly transporter involved in transport of undetermined substrate (possibly cationic amino acids) across the membrane: so responsible for the translocation of the substrate across the membrane. Probable conserved integral membrane protein Rv1999c, (MTCY39.19), len: 440 aa. Probable conserved integral membrane protein, possibly transporter of cationic amino acid, similar to many transporters, especially amino acid transporters, e.g. CAC08265.1|AL392146 putative amino acid transporter from Streptomyces coelicolor (414 aa); P39277|YJEH_ECOLI hypothetical 44.8 kDa protein from Escherichia coli (418 aa), FASTA scores, opt: 343, E(): 6.6e-15, (27.2% identity in 408 aa overlap); etc. Also similar to Rv1979c from Mycobacterium tuberculosis, FASTA score: (28.2% identity in 277 aa overlap); Rv2127, Rv0346c, Rv0522, etc. Seems to belong to the APC family. P9WQM3 No cell wall and cell processes P63349 P9WQM3 GO:0006865,GO:0016021,GO:0005886,GO:0055085,GO:0015171 P9WQM3 NC_000962.3 Mycobrowser_v5 CDS 2263998 2265038 . + 0 Rv2017 Rv2017 Thought to be involved in transcriptional mechanism. Transcriptional regulatory protein Rv2017, (MTV018.04), len: 346 aa. Transcriptional regulator. Contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature in C-terminal half, may be fortuitous. Contains probable helix-turn-helix motif at aa 18-39 (Score 2243, +6.83 SD); IPR001387 Helix-turn-helix type 3. O53463 No regulatory proteins O53463 O53463 GO:0043565 O53463 NC_000962.3 Mycobrowser_v5 CDS 2268693 2270240 . - 0 Rv2024c Rv2024c Unknown Conserved hypothetical protein Rv2024c, (MTV018.11c), len: 515 aa. Conserved hypothetical protein. Identical to N-terminal part of much larger hypothetical protein, RvD1-Rv2024c' (1606 aa), from Mycobacterium bovis BCG: CAB44655.1|Y18605|13881753|AAK46361.1|AE007059 so probably truncated. Part of RvD1 chromosomal deletion region. O53470 No conserved hypotheticals O53470 O53470 GO:0003677,GO:0009307,GO:0004519,GO:0005524 O53470 NC_000962.3 Mycobrowser_v5 CDS 2272787 2274508 . - 0 Rv2027c dosT Sensor part of the two component regulatory system DEVR/DEVS/dost; O2, NO and CO are ligands, dost is inactive when bound to oxygen Two component sensor histidine kinase DosT Rv2027c, (MTV018.14c), len: 573 aa. DosT, Histidine kinase response regulator, highly similar to others. P9WGK1 No regulatory proteins 2VZW,3ZXQ O53473 P9WGK1 GO:0005737,GO:0016021,GO:0046872,GO:0046983,GO:0000155,GO:0000160,GO:0005524 P9WGK1 NC_000962.3 Mycobrowser_v5 CDS 2289282 2289599 . - 0 Rv2044c Rv2044c Unknown Conserved hypothetical protein Rv2044c, (MTV018.31c), len: 105 aa. Conserved hypothetical protein, similar to many. O53487 No conserved hypotheticals O53487 O53487 NC_000962.3 Mycobrowser_v5 CDS 3871084 3872496 . - 0 Rv3450c eccB4 Unknown ESX conserved component EccB4. ESX-4 type VII secretion system protein. Probable membrane protein. Rv3450c, (MTCY13E12.03c), len: 470 aa. EccB4, esx conserved component, ESX-4 type VII secretion system protein, probable membrane protein (possible membrane spanning region near N-terminus). Similar to hypothetical unknowns proteins from Mycobacterium leprae e.g. O33088|MLCB628.17C|ML0054 hypothetical 51.9 KDA protein (putative membrane protein)(481 aa), FASTA scores: opt: 708, E(): 6.4e-32, (32.9% identity in 480 aa overlap); Q9CD29|ML2536 (552 aa), FASTA scores: opt: 394, E(): 1.7e-14, (33.6% identity in 503 aa overlap); etc. Also similar to other proteins from Mycobacterium tuberculosis (strains H37Rv and CDC1551) e.g. O69734|Rv3869|MTV027.04 (480 aa), FASTA scores: opt: 717, E(): 2e-32, (32.55% identity in 479 aa overlap); O05449|Rv3895c|MTCY15F10.17 (495 aa), FASTA scores: opt: 670, E(): 8.3e-30, (36.4% identity in 475 aa overlap); O5368|Rv0283|MTV035.11 (538 aa), FASTA scores: opt: 467, E(): 1.5e-18, (36.3% identity in 493 aa overlap); etc. P9WNR1 No cell wall and cell processes O06317 P9WNR1 GO:0005886,GO:0016021 P9WNR1 NC_000962.3 Mycobrowser_v5 CDS 2279129 2280124 . + 0 Rv2032 acg Unknown. May have a role for bacteria within the host environment. Conserved protein Acg Rv2032, (MTV018.19), len: 331 aa. Acg (for acr-coregulated gene), conserved protein possibly member of a superfamily of classical nitroreductases (see Purkayastha et al., 2002), similar to Rv3127 and Rv3131. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WIZ9 No conserved hypotheticals O53476 P9WIZ9 GO:0016491,GO:0055114 1.-.-.- P9WIZ9 NC_000962.3 Mycobrowser_v5 CDS 2308131 2310755 . - 0 Rv2051c ppm1 Transfers mannose from GDP-mannose to all endogenous polyprenol-phosphates. Polyprenol-monophosphomannose synthase Ppm1 Rv2051c, (MTV018.38c), len: 874 aa. Ppm1, Polyprenol-monophosphomannose synthase. Transfers mannose from GDP-Mannose to all endogenous polyprenol-phosphates in Mycobacterium tuberculosis, proven experimentally (A. Baulard, Institut Pasteur de Lille: see citation below). O53493 No cell wall and cell processes O53493 O53493 GO:0016810,GO:0016021,GO:0042158,GO:0006807,GO:0005886,GO:0016410 O53493 NC_000962.3 Mycobrowser_v5 CDS 2317169 2320753 . - 0 Rv2062c cobN Required for cobalt insertion. Cobalamin biosynthesis protein CobN Rv2062c, (MTCY49.01c, MTV019.03), len: 1194 aa. cobN, cobalamin biosynthesis protein, similar to many. O53498 No intermediary metabolism and respiration O53498 O53498 GO:0009058 O53498 NC_000962.3 Mycobrowser_v5 CDS 2323175 2324701 . + 0 Rv2066 cobI Involved in cobalamin biosynthesis. Probable bifunctional protein, CobI-COBJ fusion protein: S-adenosyl-L-methionine-precorrin-2 methyl transferase + precorrin-3 methylase Rv2066, (MTCY49.05), len: 508 aa. Probable CobI-CobJ fusion protein, S-adenosyl-L-methionine-precorrin-2 methyl transferase and precorrin-3 methylase. Similar in N-terminal half (aa 1-240) to many S-adenosyl-L-methionine-precorrin-2 methyl transferase (244 aa), and in C-terminal half (aa 240-508) to precorrin-3 methylase (254 aa). P9WGB3 No intermediary metabolism and respiration P66877 P9WGB3 GO:0030788,GO:0030789,GO:0009236 2.1.1.131,2.1.1.130 P9WGB3 NC_000962.3 Mycobrowser_v5 CDS 2333037 2333288 . - 0 Rv2076c Rv2076c Unknown Conserved hypothetical protein Rv2076c, (MTCY49.15c), len: 83 aa. Conserved hypothetical protein, similar to many. P9WLL3 No conserved hypotheticals P9WLL3 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2335355 2337325 . + 0 Rv2079 Rv2079 Unknown Conserved hypothetical protein Rv2079, (MTCY49.18), len: 656 aa. Conserved hypothetical protein; part of Mycobacterium tuberculosis protein family with Rv2542, Rv2077c, Rv2797c, Rv0963c, Rv1949c. Contains PS00120 Lipases, serine active site P9WLK7 No conserved hypotheticals Q10687 P9WLK7 P9WLK7 NC_000962.3 Mycobrowser_v5 CDS 2344411 2346180 . + 0 Rv2088 pknJ Involved in signal transduction (via phosphorylation). Can phosphorylate the peptide substrate myelin basic protein (MBP). [catalytic activity: ATP + a protein = ADP + a phosphoprotein]. Transmembrane serine/threonine-protein kinase J PknJ (protein kinase J) (STPK J) Rv2088, (MTCY49.28), len: 589 aa. PknJ, transmembrane serine/threonine-protein kinase (see citation below). Contains PS00108 Serine/Threonine protein kinases active-site signature. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases. Experimental studies show evidence of auto-phosphorylation. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Cofactor: requires divalent cations for activity. P9WI67 No regulatory proteins P65732 P9WI67 GO:0016021,GO:0005886,GO:0006468,GO:0004674,GO:0005524 2.7.11.1 P9WI67 NC_000962.3 Mycobrowser_v5 CDS 2349334 2352054 . - 0 Rv2092c helY DNA helicase activity. ATP-dependent DNA helicase HelY Rv2092c, (MTCY49.32c), len: 906 aa. HelY, ATP-dependent DNA helicase, similar to many; contains PS00017 ATP/GTP-binding site motif A, PS00402 Binding-protein-dependent transport systems inner membrane component signature. Belongs to the SKI2 subfamily of helicases. P9WMR1 No information pathways Q10701 P9WMR1 GO:0008026,GO:0003676,GO:0005524 3.6.4.- P9WMR1 NC_000962.3 Mycobrowser_v5 CDS 2358389 2360041 . + 0 Rv2100 Rv2100 Unknown Conserved hypothetical protein Rv2100, (MTCY49.40), len: 550 aa. Conserved hypothetical protein. Member of Mycobacterium tuberculosis REP13E12 repeat family with Rv1148c, Rv1945, Rv3467, Rv0094c, Rv1128c, Rv1587c, Rv1702c, Rv3466, Rv1588c. P9WLJ3 No insertion seqs and phages P64945 P9WLJ3 NC_000962.3 Mycobrowser_v5 CDS 2360240 2363281 . + 0 Rv2101 helZ Has helicase activity. Probable helicase HelZ Rv2101, (MTV020.01), len: 1013 aa. Probable helZ, helicase, similar to many. Nucleotide position 2361623 in the genome sequence has been corrected, A:C resulting in M462L. I6YCF3 No information pathways O53499 I6YCF3 GO:0003677,GO:0004386,GO:0005524 3.6.-.- I6YCF3 NC_000962.3 Mycobrowser_v5 CDS 2372630 2373823 . + 0 Rv2113 Rv2113 Unknown Probable integral membrane protein Rv2113, (MTCY261.09), len: 397 aa. Probable integral membrane protein. O33248 No cell wall and cell processes O33248 NC_000962.3 Mycobrowser_v5 CDS 2374461 2376290 . - 0 Rv2115c mpa Involved in proteasomal protein degradation Mycobacterial proteasome ATPase Mpa Rv2115c, (MTCY261.11c), len: 609 aa. Mpa, mycobacterial proteasome ATPase, similar to many. Contains PS00674 AAA-protein family signature and PS00017 ATP/GTP-binding site motif A (P-loop). Identified as a substrate for proteasomal degradation (See Pearce et al., 2006). Pup|Rv2111c and Mpa|Rv2115c interact (See Pearce et al., 2008). P9WQN5 No cell wall and cell processes 3FP9,3M91,3M9B,3M9D,3M9H P63345 P9WQN5 GO:0017111,GO:0009405,GO:0000502,GO:0005524 P9WQN5 NC_000962.3 Mycobrowser_v5 CDS 2382489 2386067 . - 0 Rv2124c metH Involved in biosynthesis of methionine (at the terminal step) [catalytic activity: 5-methyltetrahydrofolate + L-homocysteine = tetrahydrofolate + L-methionine]. 5-methyltetrahydrofolate--homocystein methyltransferase MetH (methionine synthase, vitamin-B12 dependent isozyme) (ms) Rv2124c, (MTCY261.20c), len: 1192 aa. MetH, methionine synthase, similar to many. Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Belongs to the vitamin-B12 dependent methionine synthase family. O33259 No intermediary metabolism and respiration O33259 O33259 GO:0008898,GO:0005622,GO:0009086,GO:0008705,GO:0042558,GO:0008270,GO:0031419 2.1.1.13 O33259 NC_000962.3 Mycobrowser_v5 CDS 2388616 2390085 . + 0 Rv2127 ansP1 Involved in L-asparagine transport. L-asparagine permease AnsP1 Rv2127, (MTCY261.26), len: 489 aa. AnsP1, L-asparagine permease, integral membrane protein similar to many. Contains PS00218 Amino acid permeases signature. Seems to belong to the APC family. P9WQM9 No cell wall and cell processes O33261 P9WQM9 GO:0006865,GO:0016021,GO:0005886,GO:0055085,GO:0015171 P9WQM9 NC_000962.3 Mycobrowser_v5 CDS 2390085 2390288 . + 0 Rv2128 Rv2128 Unknown Conserved transmembrane protein Rv2128, (MTCY26.27), len: 67 aa. Conserved transmembrane protein, similar to many. O33262 No cell wall and cell processes O33262 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2409697 2410641 . - 0 Rv2151c ftsQ This protein may be involved in septum formation. Possible cell division protein FtsQ Rv2151c, (MTCY270.17), len: 314 aa. Possible ftsQ, cell division protein, with some homology to FTSQ_STRGR|P45503 cell division protein ftsq homolog from Streptomyces griseus (208 aa), FASTA scores: opt: 204, E(): 4e-05; (30.6% identity in 193 aa overlap). P9WNA1 No cell wall and cell processes P64168 P9WNA1 GO:0007049,GO:0016021,GO:0005886,GO:0000917 P9WNA1 NC_000962.3 Mycobrowser_v5 CDS 2392517 2393320 . - 0 Rv2131c cysQ Can dephosphorylate a broad range of substrates. Likely involved in sulfur metabolism, controlling the pool of 3'-phosphoadenosine 5'-phosphate (pap) and 3'-phosphoadenoside 5'-phosphosulfate (PAPS) [catalytic activity: adenosine 3',5'-bisphosphate + H2O = adenosine 5'-phosphate + phosphate]. Has also been shown to have myo-inositol 1-phosphatase [catalytic activity: myo-inositol 1-phosphate + H(2)O = myo-inositol + phosphate] and fructose 1,6-bisphosphatase [catalytic activity: D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate] activities. Monophosphatase CysQ Rv2131c, (MTCY270.37), len: 267 aa. CysQ, monophosphatase, equivalent to CYSQ_MYCLE|P46726 cysQ protein homolog from Mycobacterium leprae (289 aa), FASTA scores: opt: 1374, E(): 0, (77.3% identity in 264 aa overlap). Contains inositol monophosphatase family signature 1 (PS00629), significance uncertain. Seems to belong to the inositol monophosphatase family. Cofactor: Mg2+. Inhibited by Li+; PAPase activity is inhibited by Na+ and K+, but IMPase activity is not (See Gu et al., 2006; Hatzios et al., 2008). P9WKJ1 No intermediary metabolism and respiration P65163 P9WKJ1 GO:0046872,GO:0004437 3.1.3.- P9WKJ1 NC_000962.3 Mycobrowser_v5 CDS 2396008 2396838 . - 0 Rv2136c Rv2136c Unknown Possible conserved transmembrane protein Rv2136c, (MTCY270.32), len: 276 aa. Possible conserved transmembrane protein, very similar to hypothetical Mycobacterium leprae protein Q49783. FASTA best: Q49783 B2126_C2_190 opt: 1023, E(): 0; (82.4% identity in 187 aa over lap) similar to BACA_ECOLI P31054 bacitracin resistance protein (273 aa) opt: 477, E(): 7e-26, (35.6% identity in 267 aa overlap) P9WFF9 No cell wall and cell processes O06239 P9WFF9 GO:0016311,GO:0016021,GO:0009252,GO:0005886,GO:0008360,GO:0046677,GO:0050380,GO:0007047 3.6.1.27 NC_000962.3 Mycobrowser_v5 CDS 2400376 2401722 . - 0 Rv2141c Rv2141c Unknown Conserved protein Rv2141c, (MTCY270.27), len: 448 aa. Conserved protein. Shows some similarity to conserved hypothetical proteins and to acetylornithine deacetylase and succinyl-diaminopimelate desuccinylase and contains ArgE/dapE/ACY1/CPG2/yscS family signature 1 (PS00758). FASTA best: CBPS_YEAST P27614 carboxypeptidases precursor (576 aa) opt: 234, E(): 4.3e-08; (24.3% identity in 412 aa overlap). Previously named dapE2. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). L7N684 No intermediary metabolism and respiration Q7D7G7 L7N684 GO:0005515,GO:0006508,GO:0008237 L7N684 NC_000962.3 Mycobrowser_v5 CDS 2417472 2419004 . - 0 Rv2157c murF Involved in cell wall formation; peptidoglycan biosynthesis. Probable UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanyl ligase MurF Rv2157c, (MTCY270.11), len: 510 aa. Probable murF, UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanyl ligase (UDP-murnac-pentapeptide synthetase) (see citation below), also related to other Mycobacterium tuberculosis mur gene products. FASTA best: MURF_ECOLI|P11880 (452 aa), opt: 515, E(): 2.6e-24, (31.9% identity in 511 aa overlap). P9WJL1 No cell wall and cell processes P0A5L4 P9WJL1 GO:0047480,GO:0008766,GO:0007049,GO:0051301,GO:0007047,GO:0005737,GO:0009252,GO:0008360,GO:0005524 6.3.2.10 P9WJL1 NC_000962.3 Mycobrowser_v5 CDS 2425048 2427087 . - 0 Rv2163c pbpB Involved in peptidoglycan biosynthesis Probable penicillin-binding membrane protein PbpB Rv2163c, (MTCY270.05), len: 679 aa. Probable pbpB, penicillin-binding membrane protein, similar to many bacterial PBP2 proteins e.g. P11882|PBP2_NEIME|PENA|NMA2072|NMB0413 penicillin-binding protein 2 (pbp-2) from Neisseria meningitidis (serogroups a and B) (581 aa), FASTA scores: opt: 665, E(): 1.6e-31, (33.2% identity in 591 aa overlap); etc. Also similar to Rv0016c and Rv2864c from Mycobacterium tuberculosis (2.8e-10). Contains PS00017 possible ATP/GTP-binding site motif A (P-loop) near C-terminus. FASTA best: PBP2_NEIME P11882 penicillin-binding protein 2 (pbp-2). (581 aa) opt: 665, E(): 1.6e-31; (33.2% identity in 591 aa overlap). FtsW|Rv2154c interacts with PbpB|Rv2163c and FtsZ|RvRv2150c (See Datta et al., 2006). Cleavage of PbpB|Rv2163c by Rv2869c under conditions of oxidative stress is prevented by Wag31|Rv2145c (See Mukherjee et al., 2009). L0T911 No cell wall and cell processes O06214 L0T911 GO:0009273,GO:0008658 L0T911 NC_000962.3 Mycobrowser_v5 CDS 2432235 2432855 . + 0 Rv2170 Rv2170 Acetylation, substrate unknown GCN5-related N-acetyltransferase Rv2170, (MTV021.03), len: 206 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain in C-terminal part. See Vetting et al. 2005. Equivalent to hypothetical protein ML0903 (210 aa) from Mycobacterium leprae. FASTA scores: ML0903 conserved hypothetical protein (210 aa) opt: 1045, E(): 9.1e-57; 77.143% identity in 210 aa overlap. >emb|CAA18679.1| (AL022602) >gi|13092973|emb|CAC31284.1| (AL583920). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53504 No intermediary metabolism and respiration O53504 O53504 GO:0008152,GO:0008080 O53504 NC_000962.3 Mycobrowser_v5 CDS 2626654 2626980 . - 0 Rv2348c Rv2348c Unknown Hypothetical protein Rv2348c, (MTCY98.17c), len: 108 aa. Hypothetical unknown protein. P95244 No unknown P95244 NC_000962.3 Mycobrowser_v5 CDS 2437941 2439140 . + 0 Rv2176 pknL Involved in signal transduction (via phosphorylation). May be involved in transcriptional mechanism. Probable transmembrane serine/threonine-protein kinase L PknL (protein kinase L) (STPK L) Rv2176, (MTV021.09), len: 399 aa. Probable pknL, transmembrane serine/threonine-protein kinase (see citation below), similar to many e.g. MLCB1770_9 (622 aa). Lacks C-terminal domain and ends with putative transmembrane segment. Contains PS00108 Serine/Threonine protein kinases active-site signature. FASTA scores: Z70722|MLC B1770_9 Mycobacterium leprae cosmid B1770 (622 aa) opt: 732, E(): 5.9e-23; 44.4% identity in 266 aa overlap. Also similar to several Mycobacterium tuberculosis STPK proteins e.g. Rv0014c|PKNB, Rv0015c|PKNA, Rv1743|PKNE, Rv1266c|PKNH etc. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases. P9WI63 No regulatory proteins O53510 P9WI63 GO:0016021,GO:0005886,GO:0006468,GO:0004674,GO:0005524 2.7.11.1 P9WI63 NC_000962.3 Mycobrowser_v5 CDS 2440332 2441720 . - 0 Rv2178c aroG Chorismate biosynthesis 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase AroG (DAHP synthetase, phenylalanine-repressible) Rv2178c, (MTV021.11c), len: 462 aa. aroG, 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase similar to many, especially those from plants. FASTA scores: Y15113|M C3DDAH7P_1Morinda citrifolia mRNA for 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (535 aa) opt: 1421, E(): 0; 48.3% identity in 443 aa overlap. O53512 No intermediary metabolism and respiration 2B7O,2W19,2W1A,3KGF,3NUD,3NUE,3NV8,3PFP O53512 O53512 GO:0009073,GO:0003849 2.5.1.54 O53512 NC_000962.3 Mycobrowser_v5 CDS 2444586 2445329 . - 0 Rv2182c Rv2182c Transfer of fatty acyl groups 1-acylglycerol-3-phosphate O-acyltransferase Rv2182c, (MTV021.15c), len: 247 aa. Probable 1-acylglycerol-3-phosphate O-acyltransferase, similar to many e.g. in Streptomyces. Contains PS00017 ATP/GTP-binding site motif A (P-loop). FASTA scores: pir||T35503 1-acylglycerol-3-phosphate O-acyltransferase homolog SC6E10.16c - Streptomyces coelicolor >gi|5689932|emb|CAB51970.1| (AL109661) hypothetical protein [Streptomyces coelicolor A3(2)] Length = 262, Expect = 6e-61 (54% identity in 215 aa overlap). O53516 No conserved hypotheticals O53516 O53516 GO:0008152,GO:0003841 2.3.1.51 O53516 NC_000962.3 Mycobrowser_v5 CDS 2461504 2462148 . - 0 Rv2197c Rv2197c Unknown Probable conserved transmembrane protein Rv2197c, (MTCY190.08c), len: 214 aa. Probable conserved transmembrane protein, equivalent to ML0878 conserved hypothetical protein (212 aa) of Mycobacterium leprae. FASTA scores: opt: 858; 62.559% identity in 211 aa overlap CAC31259.1|(AL583920). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WLI9 No cell wall and cell processes P9WLI9 GO:0005886,GO:0016021 P9WLI9 NC_000962.3 Mycobrowser_v5 CDS 3491808 3492122 . - 0 Rv3126c Rv3126c Unknown Hypothetical protein Rv3126c, (MTCY164.36c), unknown, len: 104 aa. Hypothetical unknown protein. Shortened version of MTCY164.36c, avoiding overlap. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WL09 No conserved hypotheticals P9WL09 EMB NC_000962.3 Mycobrowser_v5 CDS 2448160 2449962 . + 0 Rv2187 fadD15 Function unknown, but involved in lipid degradation. Long-chain-fatty-acid-CoA ligase FadD15 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv2187, (MTV021.20), len: 600 aa. fadD15, long-chain-fatty-acid-CoA ligase, similar to several e.g. P44446|LCFH_HAEIN putative long-chain-fatty-acid--CoA ligase from Haemophilus influenzae (607 aa), FASTA scores: (607 aa) opt: 992, E(): 0, (31.5% identity in 578 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family. O53521 No lipid metabolism O53521 O53521 GO:0008152,GO:0004467 6.2.1.3 O53521 NC_000962.3 Mycobrowser_v5 CDS 2453819 2455756 . + 0 Rv2191 Rv2191 Unknown Conserved hypothetical protein Rv2191, (MTCY190.02), len: 645 aa. Conserved hypothetical protein, similar to SW:DP3A_B ACSU P13267 DNA polymerase III, alpha chain (31.3% identity in 249 aa overlap) and SW:UVRC_ECOLI P07028 excinuclease ABC subunit C (25.7% identity in 230 aa overlap). Also similar to M. tuberculosis Rv3711c (dnaQ DNA polymerase III e chain) and Rv1420 (uvrC excinuclease ABC subunit C) P9WLJ1 No information pathways Q10384 P9WLJ1 GO:0006281,GO:0006260,GO:0003887,GO:0004527,GO:0005622,GO:0003677 P9WLJ1 NC_000962.3 Mycobrowser_v5 CDS 2459678 2461327 . + 0 Rv2196 qcrB Respiration Probable ubiquinol-cytochrome C reductase QcrB (cytochrome B subunit) Rv2196, (MTCY190.07), len: 549 aa. Probable qcrB, Ubiquinol-cytochrome C reductase cytochrome B subunit (cytB), integral membrane protein, low similarity in amino-terminal half to cytochrome b subunits, highly similar at C-terminus to SW:12KD_MYCLE P15878 12 KD protein PIR:S08427 (86.9% identity in 153 aa overlap). FASTA scores: sp|Q45658|QCRB_BACST menaquinol-cytochrome C reductase (224 aa) opt: 341, E(): 6.8e-15; 28.0% identity in 207 aa overlap P9WP37 No intermediary metabolism and respiration P9WP37 GO:0016021,GO:0046872,GO:0016491,GO:0005886,GO:0022904,GO:0006810,GO:0009055 NC_000962.3 Mycobrowser_v5 CDS 2687128 2687973 . + 0 Rv2393 che1 Possibly involved in inserting FE2+ into sirohydrochlorin to produce siroheme, required for SIRA (Rv2391) function Ferrochelatase Che1 Rv2393, (MTCY253.28c), len: 281 aa. Che1, ferrochelatase (See Pinto et al., 2007). Conserved protein, with some similarity to Q9L2E8|SC7A8.10c putative secreted protein from Streptomyces coelicolor (274 aa), FASTA scores: opt: 407, E(): 2.8e-18, (37% identity in 246 aa overlap); CAC38793|SCI39.05 Conserved hypothetical protein from Streptomyces coelicolor (305 aa), FASTA scores: opt: 394, E(): 2e-17, (35.0% identity in 251 aa overlap); AAK44492|MT0272 Chalcone/stilbene synthase family protein from Mycobacterium tuberculosis (247 aa), FASTA scores: opt: 350, E(): 9.2e-15, (34.0% identity in 235 aa overlap); P95216|Rv0259c|MTCY06A4.03c|Z86089 hypothetical protein from Mycobacterium tuberculosis (247 aa), FASTA scores: opt: 345, E(): 1.9e-14,(33.6% identity in 235 aa overlap). P71751 No intermediary metabolism and respiration P71751 P71751 GO:0016829,GO:0046872,GO:0009236 P71751 NC_000962.3 Mycobrowser_v5 CDS 2468231 2468923 . + 0 Rv2203 Rv2203 Unknown Possible conserved membrane protein Rv2203, (MTCY190.14), len: 230 aa. Possible conserved membrane protein; has single hydrophobic stretch from aa 75 to 97 and is equivalent to Mycobacterium leprae ML0872 putative membrane protein (171 aa). FASTA scores: opt: 821, E(): 3.4e-42; 72.353% identity in 170 aa overlap - CAC31253.1| (AL583920). 2468411. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WLI7 No cell wall and cell processes P9WLI7 GO:0016021 P9WLI7 NC_000962.3 Mycobrowser_v5 CDS 2477190 2478326 . + 0 Rv2212 Rv2212 Possibly involved in cAMP synthesis [catalytic activity: ATP = 3',5'-cyclic AMP + diphosphate] Adenylyl cyclase (ATP pyrophosphate-lyase) (adenylate cyclase) Rv2212, (MTCY190.23), len: 378 aa. Adenylyl cyclase (See Abdel Motaal et al., 2006). Some similarity to e.g. SW:CYAA_STRCO P40135 adenylate cyclase (29.2% identity in 291 aa overlap); ttg at 24614 in MTCY190 has a better rbs. Contains possible helix-turn-helix motif at aa 64- 85, (+2.72 SD). Also similar to Rv1264 and Rv1647 P9WMU7 No intermediary metabolism and respiration P64265 P9WMU7 GO:0009190,GO:0016849 P9WMU7 NC_000962.3 Mycobrowser_v5 CDS 2478338 2479885 . + 0 Rv2213 pepB Protein degradation Probable aminopeptidase PepB Rv2213, (MTCY190.24), len: 515 aa. Probable pepB, leucine aminopeptidase, similar to many e.g. SW:AMPA_ECOLI P11648 aminopeptidase a/I, (41.4% identity in 309 aa overlap). Equivalent to Z98741|MLCB22_6 Mycobacterium leprae cosmid B22; Am (524 aa), FASTA scores: opt: 2793, E(): 0; 83.1% identity in 522 aa overlap. Contains PS00631 Cytosol aminopeptidase signature, ntdaegrl. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P9WHT3 No intermediary metabolism and respiration Q10401 P9WHT3 GO:0005737,GO:0030145,GO:0008235,GO:0006508,GO:0004177 3.4.11.1 P9WHT3 NC_000962.3 Mycobrowser_v5 CDS 2481965 2483626 . + 0 Rv2215 dlaT Involved in tricarboxylic acid cycle; converts pyruvate to acetyl-CoA and CO2. Also involved in antioxidant defense; LPDC|Rv0462, DLAT|Rv2215, AHPD|Rv2429, and AHPC|Rv2428 constitute an NADH-dependent peroxidase and peroxynitrite reductase that provides protection against oxidative stress. DlaT, dihydrolipoamide acyltransferase, E2 component of pyruvate dehydrogenase Rv2215, (MTCY190.26), len: 553 aa. DlaT, dihydrolipoamide acyltransferase, E2 component of pyruvate dehydrogenase, proven biochemically (see Tian et al. 2005), similar to e.g. SW:O PD2_ACHLA P35489 dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex (35.3% identity in 552 aa overlap); contains PS00189 2-oxo acid dehydrogenases acyltransferase component lipoyl binding site. Rhodanine compounds inhibit DlaT|Rv2215 and can kill non-replicating mycobacteria in mouse bone marrow-derived macrophages (See Bryk et al., 2008). LpdC|Rv0462 co-immunoprecipitates with DlaT|Rv2215 (in lpdC|Rv0462 mutant) and with BkdC|Rv2495c (in dlaT|Rv2215 mutant) (See Venugopal et al., 2011). P9WIS7 No intermediary metabolism and respiration P65633 P9WIS7 GO:0004149,GO:0031405,GO:0005515,GO:0006099,GO:0016209 2.3.1.61 P9WIS7 NC_000962.3 Mycobrowser_v5 CDS 2487615 2489051 . + 0 Rv2220 glnA1 Involved in glutamine biosynthesis [catalytic activity: ATP + L-glutamate + NH(3) = ADP + glutamine + orthophosphate]. Glutamine synthetase GlnA1 (glutamine synthase) (GS-I) Rv2220, (MTCY190.31, MTCY427.01), len: 478 aa. glnA1, glutamine synthetase class I (see Tullius et al., 2001), similar to many e.g. GLNA_STRCO|P15106 from Streptomyces coelicolor, FASTA score: (71.4% identity in 475 aa overlap); etc. Also similar to three other potential glutamine synthetases in Mycobacterium tuberculosis: Rv2222c|glnA2, Rv2860c|glnA4, and Rv1878|glnA3. Contains PS00180 Glutamine synthetase signature 1, PS00181 Glutamine synthetase putative ATP-binding region signature, and PS00182 Glutamine synthetase class-I adenylation site. Belongs to the glutamine synthetase family. Note has shown to be essential for M. tuberculosis virulence. P9WN39 No intermediary metabolism and respiration 1HTO,1HTQ,2BVC,2WGS,2WHI P0A590 P9WN39 GO:0005737,GO:0004356,GO:0006542,GO:0009399,GO:0005524 6.3.1.2 P9WN39 NC_000962.3 Mycobrowser_v5 CDS 2489369 2492353 . - 0 Rv2221c glnE Regulatory protein involved in the regulation of glutamine synthetase activity. Adenylylation and deadenylylation of glutamine synthetase. Possibly regulates GLNB|Rv2919c [catalytic activity: ATP + [L-glutamate:ammonia ligase (ADP-forming)] = pyrophosphate + adenylyl-[L-glutamate:ammonia ligase (ADP-forming)]]. Glutamate-ammonia-ligase adenylyltransferase GlnE (glutamine-synthetase adenylyltransferase) Rv2221c, (MTCY190.32c, MTCY427.02c), len: 994 aa. glnE, glutamate-ammonia-ligase adenylyltransferase (see citations below), similar to others e.g. GLNE_ECOLI|P30870 glutamate-ammonia-ligase adenylyltransferase from Escherichia coli, FASTA score: (24.4% identity in 721 aa overlap); GLNE_HAEIN|P44419 Glutamate-ammonia-ligase adenylyltransferase from Haemophilus influenzae (981 aa), FASTA score: (28.1% identity in 199 aa overlap); etc. Note that initiation codon uncertain. P9WN27 No intermediary metabolism and respiration P69942 P9WN27 GO:0008882,GO:0005524 2.7.7.42 P9WN27 NC_000962.3 Mycobrowser_v5 CDS 2495461 2497023 . - 0 Rv2224c caeA Converts unknown esters to corresponding free acid and alcohol Probable carboxylesterase CaeA Rv2224c, (MTCY427.05c), len: 520 aa. Probable caeA, carboxylesterase; has signal sequence and lipoprotein motif at N-terminal end. Very similar to three proteases/peptidases from Streptomyces spp.: L42758, L42759, L27466. FASTA score: L4 2758|STMSLPD STMSLPD NID: g940302 - Streptomyces (539 aa) opt: 1032 E(): 0, (37.5% identity in 533 aa overlap). Similar to hypothetical protein SW:YZZE_ECOLI P34211 (27.7% identity in 412 aa overlap) and highly similar to Rv2224c and Rv2672 (49.3% identity in 507 aa overlap); contains PS00013, Prokaryotic membrane lipoprotein lipid attachment site, and PS00120 Lipases, serine active site. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). Predicted to be an outer membrane protein (See Song et al., 2008). P9WHR3 No cell wall and cell processes P65823 P9WHR3 P9WHR3 NC_000962.3 Mycobrowser_v5 CDS 2497742 2498587 . + 0 Rv2225 panB Involved in pantothenate biosynthesis. 3-methyl-2-oxobutanoate hydroxymethyltransferase PanB Rv2225, (MTCY427.06), len: 281 aa. panB, 3-methyl-2-oxobutanoate hydroxymethyltransferase, similar to PANB_ECOLI|P31057 3-methyl-2-oxobutanoate hydroxymethyltransferase from Escherichia coli (45.9% identity in 257 aa overlap). Identified as a substrate for proteasomal degradation (See Pearce et al., 2006). P9WIL7 No intermediary metabolism and respiration 1OY0 P0A5Q8 P9WIL7 GO:0005737,GO:0046872,GO:0015940,GO:0003864 2.1.2.11 P9WIL7 NC_000962.3 Mycobrowser_v5 CDS 2501644 2502738 . - 0 Rv2228c Rv2228c Unknown Multifunctional protein. Has RNASE H, alpha-ribazole phosphatase, and acid phosphatase activities. Rv2228c, (MTCY427.09c), len: 364 aa. Multifunctional protein with RNase H, alpha-ribazole phosphatase, and acid phosphatase activities. Some similarity to phosphoglycerate mutase and ribonuclease H. Similar to CAB88177.1|AL352972 putative bifunctional protein (ribonuclease H/phosphoglycerate mutase) from Streptomyces coelicolor A3(2) (497 aa); Smith-Waterman scores: 107 bits (424), Expect = 4e-41 Identities = 160/485 (32%). Also similar in C-terminal part to Rv2419c and Rv2135c. P9WLH5 No information pathways 3HST P64955 P9WLH5 GO:0004523,GO:0003676 P9WLH5 NC_000962.3 Mycobrowser_v5 CDS 2504605 2505699 . - 0 Rv2231c cobC Involved in cobalamin biosynthesis Possible aminotransferase CobC Rv2231c, (MTCY427.12c), len: 364 aa. Possible cobC, aminotransferase. Note that initiation codon uncertain. Similar to CobC aminotransferases e.g. sp|P21633|COBC_PSEDE COBC protein (333 aa) opt: 277, E(): 1.7e-11; 28.8% identity in 313 aa overlap and also to e.g. SW:HIS8_ECOLI P06986 histidinol-phosphate aminotransferase (27.0% identity in 289 aa overlap), contains PS00105 aminotransferases class-I pyridoxal-phosphate attachment site. Real Mycobacterium tuberculosis histidinol-phosphate aminotransferase, hisC, is Rv1600 (MTCY336.04c). P9WQ89 No intermediary metabolism and respiration P63500 P9WQ89 GO:0005737,GO:0030170,GO:0008483,GO:0009058 2.6.1.- P9WQ89 NC_000962.3 Mycobrowser_v5 CDS 2506278 2507153 . + 0 Rv2232 ptkA Involved in signal transduction (via phosphorylation). Can phosphorylate PTPA|Rv2234 and the peptide substrate myelin basic protein (MBP) [catalytic activity: ATP + a protein = ADP + a phosphoprotein] Protein tyrosine kinase transcriptional regulatory protein PtkA Rv2232, (MTCY427.13), len: 291 aa. PtkA, protein tyrosine kinase, similar to members of haloacid dehalogenase-like family from several bacteria and to putative phosphatases e.g. Q9I767 and AAK78398. Contains N-terminal extension. FASTA scores: Q9I767 hypothetical protein PA0065 (221 aa) opt: 439 E(): 3.2e-18; 38.679% identity (40.196% ungapped) in 212 aa overlap; >>tr|AAK78398 Predicted phosphatase, had family (216 aa) opt: 427, E(): 1.5e-17; 34.762% identity (35.437% ungapped) in 210 aa overlap. Replaces previous Rv2232 and Rv2233. Predicted to be an outer membrane protein (See Song et al., 2008). P9WPI9 No regulatory proteins P68911 P9WPI9 GO:0008967,GO:0008152 P9WPI9 NC_000962.3 Mycobrowser_v5 CDS 2512539 2515244 . + 0 Rv2241 aceE Involved in energy metabolism; contributes to acetyl-CoA production as part of pyruvate dehydrogenase complex [catalytic activity: pyruvate + lipoamide = S-acetyl-dihydro-lipoamide + CO(2)]. Pyruvate dehydrogenase E1 component AceE (pyruvate decarboxylase) (pyruvate dehydrogenase) (pyruvic dehydrogenase) Rv2241, (MTCY427.22), len: 901 aa. AceE, pyruvate dehydrogenase E1 component, similar to others e.g. ODP1_ECOLI|P06958 pyruvate dehydrogenase E1 component from Escherichia coli, FASTA score: (51.2% identity in 891 aa overlap); etc. P9WIS9 No intermediary metabolism and respiration Q10504 P9WIS9 GO:0055114,GO:0004739,GO:0006096 1.2.4.1 P9WIS9 NC_000962.3 Mycobrowser_v5 CDS 2516787 2517695 . + 0 Rv2243 fabD Catalyzes malonyl-CoA-ACP transacylase (MCAT) activity using holo-ACPM as substrate for transacylation [catalytic activity: malonyl-CoA + [acyl-carrier protein] = CoA + malonyl-[acyl-carrier protein]]. Malonyl CoA-acyl carrier protein transacylase FabD (malonyl CoA:ACPM acyltransferase) (MCT) Rv2243, (MTCY427.24), len: 302 aa. FabD (alternate gene name: mtFabD), malonyl CoA-acyl carrier protein transacylase (see citations below), highly similar to e.g. A57356 acyl-CoA carrier protein malonyltransferase from Streptomyces coelicolor (316 aa), FASTA score: opt: 955, E(): 0, (52.6% identity in 304 aa overlap); FABD_HAEIN|P43712 malonyl CoA-acyl carrier protein transacylase from Haemophilus influenzae, FASTA score: (30.5% identity in 308 aa overlap); and FABD_ECOLI|P25715 from Escherichia coli, FASTA score: (31.4% identity in 309 aa overlap). Identified as a substrate for proteasomal degradation (See Pearce et al., 2006). P9WNG5 No lipid metabolism 2QC3,2QJ3 P63458 P9WNG5 GO:0006633,GO:0004314 2.3.1.39 INH P9WNG5 NC_000962.3 Mycobrowser_v5 CDS 2572076 2573008 . - 0 Rv2300c Rv2300c Function unknown Conserved protein Rv2300c, (MTCY339.09), len: 310 aa (start uncertain). Conserved protein, similar to others e.g. Q9RXY2|DR0172 conserved hypothetical protein from Deinococcus radiodurans (271 aa), FASTA scores: opt: 306, E(): 1.3e-12, (34.6% identity in 229 aa overlap); Q9HZH1|PA3037 hypothetical protein from Pseudomonas aeruginosa (288 aa), FASTA scores: opt: 248, E(): 7.9e-09, (31.5% identity in 238 aa overlap); Q9PDL8|XF1361 hypothetical protein from Xylella fastidiosa (279 aa), FASTA scores: opt: 236, E(): 4.6e-08, (29.7% identity in 249 aa overlap); U70053|XCU70053_3 GumP protein from Xanthomonas campestris (282 aa), FASTA scores: opt: 222, E(): 3.7e-07, (30.1% identity in 248 aa overlap); etc. P9WLD7 No conserved hypotheticals P9WLD7 GO:0016787 P9WLD7 NC_000962.3 Mycobrowser_v5 CDS 2519396 2520712 . + 0 Rv2246 kasB Involved in fatty acid biosynthesis (mycolic acids synthesis); involved in meromycolate extension. Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP [catalytic activity: acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = 3-oxoacyl-[acyl-carrier protein] + [acyl-carrier protein] + CO(2)]. 3-oxoacyl-[acyl-carrier protein] synthase 2 KasB (beta-ketoacyl-ACP synthase) (KAS I) Rv2246, (MTCY427.27), len: 438 aa. KasB, beta-ketoacyl-ACP synthase, involved in meromycolate extension (see citations below). Highly similar or similar to others e.g. L43074|STMFABD3|g870805 beta-ketoacyl-ACP synthase from Streptomyces glaucescens (423 aa), FASTA scores: opt: 1091, E(): 0, (44.7% identity in 416 aa overlap); FABF_ECOLI|P39435 3-oxoacyl-[acyl-carrier-protein] synthase II from Escherichia coli, FASTA score: (37.0% identity in 411 aa overlap); FABB_HORVU|P23902 3-oxoacyl-[acyl-carrier-protein] synthase I, FASTA score: (32.5% identity in 415 aa overlap); etc. Strongest similarity to upstream ORF Rv2245|kasA|MTCY427.26 3-oxoacyl-[acyl-carrier-protein] synthase 1 from Mycobacterium tuberculosis (416 aa), FASTA score: (66.3% identity in 409 aa overlap). Belongs to the beta-ketoacyl-ACP synthases family. P9WQD7 No lipid metabolism 2GP6 P63456 P9WQD7 GO:0005737,GO:0006633,GO:0004315 2.3.1.41 P9WQD7 NC_000962.3 Mycobrowser_v5 CDS 2525565 2526992 . + 0 Rv2251 Rv2251 Electron acceptor Possible flavoprotein Rv2251, (MTV022.01), len: 475 aa. Possible flavoprotein, probably continuation of Rv2250A, similar to MTCY164.18 from Mycobacterium tuberculosis and to several alkyldihydroxyacetonephosphate synthases (e.g. O00116). Also some similarity to D-lactate dehydrogenases. FASTA scores: sptr|O05784|O05784 hypothetical 56.5 kDa protein. (527 aa) opt: 1019 E(): 0; (38.6% identity in 487 aa overlap) and sp|O00116|ADAS_HUMAN alkyldihydroxyaceton ephosphate synthase precursor (658 aa) opt: 558 E(): 6.2e-27; (31.3% identity in 447 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). L0TBR2 No intermediary metabolism and respiration O53525 L0TBR2 GO:0016491,GO:0050660 L0TBR2 NC_000962.3 Mycobrowser_v5 CDS 2532245 2533330 . + 0 Rv2259 mscR Involved in the reduction of S-nitroglutathione S-nitrosomycothiol reductase MscR Rv2259, (MTV022.09), len: 361 aa. MscR, S-nitrosomycothiol reductase (see Vogt et al., 2003), similar to several zinc-containing alcohol dehydrogenases especially from Amycolatopsis methanolica P80094 (360 aa), FASTA scores: sp|P80094|FADH_AMYME NAD/mycothiol-dependent formaldehyde dehydrogenase (MD-FALDH) Length = 360, Expect = e-156, Identities = 268/358 (74%). Also similar to Rv0162c, (MTCI28.02c, 35.0% identity in 371 aa overlap). Contains PS00059 Zinc-containing alcohol dehydrogenases signature. Note previously known as adhE2 O53533 No intermediary metabolism and respiration O53533 O53533 GO:0016491,GO:0008270,GO:0055114 1.2.1.- O53533 NC_000962.3 Mycobrowser_v5 CDS 2534470 2535552 . - 0 Rv2262c Rv2262c Function unknown; thought to be involved in lipid metabolism. Conserved hypothetical protein Rv2262c, (MTV022.12c), len: 360 aa. Conserved hypothetical protein, with function unknown but some similarity to N-terminal 70% of P23930|P77703|LNT_ECOLI|cute|B0657 apolipoprotein N-acyltransferase from Escherichia coli strain K12 (512 aa), FASTA scores: opt: 239, E(): 1.6e-07, (30.4% identity in 359 aa overlap). Note that neighboring ORF shows similarity to N -terminal part of PCC6803 apolipoprotein N-acyltransferase from Synechocystis sp., suggesting possibility of frameshift. Sequence of clones from two sources has been checked but no error found. Appear to be two extra bases at position 1876970 compared to CDC1551 strain. O53536 No lipid metabolism O53536 O53536 GO:0016810,GO:0016021,GO:0042158,GO:0006807,GO:0016410 O53536 NC_000962.3 Mycobrowser_v5 CDS 2540104 2541390 . + 0 Rv2266 cyp124 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Probable cytochrome P450 124 Cyp124 Rv2266, (MT2328, MTCY339.44c), len: 428 aa. Probable cyp124, cytochrome P450, similar to e.g. G405543 cytochrome P450 (406 aa), FASTA scores, opt: 763,E(): 0, (35.4% identity in 393 aa overlap), similar to e.g. MTCY50.26, 33.8% identity in 370 aa overlap P9WPP3 No intermediary metabolism and respiration 2WM4,2WM5 P0A516 P9WPP3 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPP3 NC_000962.3 Mycobrowser_v5 CDS 2553173 2554831 . + 0 Rv2281 pitB Involved in phosphate transport. Putative phosphate-transport permease PitB Rv2281, (MTCY339.29c), len: 552 aa. Putative pitB, phosphate-transport permease, integral membrane protein, similar to YG04_HAEIN P45268 putative phosphate permease hi1604 (420 aa). FASTA scores, opt: 484, E(): 5e-23, (33.5% identity in 498 aa overlap) also to G399598 amphotropic murine retrovirus receptor (656 aa) FASTA scores, opt: 453, E(): 5.8e-21, (26.8% identity in 645 aa overlap). Also similar to Rv0545c|pitA from M. tuberculosis. Belongs to the pit subfamily. P9WIA5 No cell wall and cell processes P65712 P9WIA5 GO:0016021,GO:0006817,GO:0005886,GO:0005315 NC_000962.3 Mycobrowser_v5 CDS 2561675 2562457 . + 0 Rv2289 cdh Involved in phospholipid biosynthesis [catalytic activity: CDP-diacylglycerol + H(2)O = CMP + phosphatidate]. Probable CDP-diacylglycerol pyrophosphatase Cdh (CDP-diacylglycerol diphosphatase) (CDP-diacylglycerol phosphatidylhydrolase) Rv2289, (MTCY339.21c), len: 260 aa. Probable cdh, CDP-diacylglycerol pyrophosphatase, similar to CDH_SALTY|P26219 cdp-diacylglycerol pyrophosphatase (251 aa), FASTA scores: opt: 395, E(): 5.9e-20, (33.5% identity in 221 aa overlap). P9WPG9 No lipid metabolism P63751 P9WPG9 GO:0016021,GO:0008654,GO:0005886,GO:0008715 3.6.1.26 P9WPG9 NC_000962.3 Mycobrowser_v5 CDS 2565327 2566550 . + 0 Rv2294 Rv2294 Unknown Probable aminotransferase Rv2294, (MTCY339.16c), len: 407 aa. Probable aminotransferase, similar to others in M. tuberculosis e.g. MTV030_19, also similar to PATB_BACSU|Q08432 putative aminotransferase b from Bacillus subtilis (387 aa), FASTA scores: opt: 563, E(): 2.8e-29, (31.4% identity in 408 aa overlap); and to MALY_ECOLI|P23256 maly protein from Escherichia coli (390 aa), FASTA scores: opt: 530, E(): 3.6e-27, (31.3% identity in 384 aa overlap). Belongs to class-II of pyridoxal-phosphate-dependent aminotransferases. P9WQ83 No intermediary metabolism and respiration P63502 P9WQ83 GO:0009086,GO:0030170,GO:0016769,GO:0004121 4.4.1.8 P9WQ83 NC_000962.3 Mycobrowser_v5 CDS 2570059 2572002 . - 0 Rv2299c htpG Molecular chaperone involved in protein folding. Has ATPase activity. Probable chaperone protein HtpG (heat shock protein) (HSP90 family protein) (high temperature protein G) Rv2299c, (MTCY339.11), len: 647 aa. HtpG, probable chaperone, heat shock protein 90 family. Similar to HTPG_BACSU|P46208 heat shock protein htpG homologue from Bacillus subtilis (626 aa), FASTA scores: opt: 1551, E(): 0, (39.6% identity in 631 aa overlap). Contains possible helix-turn-helix motif at aa 519-540 (+3.77 SD). P9WMJ7 No virulence, detoxification, adaptation P64411 P9WMJ7 GO:0005737,GO:0006457,GO:0006950,GO:0051082,GO:0005524 P9WMJ7 NC_000962.3 Mycobrowser_v5 CDS 2574096 2575019 . - 0 Rv2303c Rv2303c Could be involved in antibiotic-resistance. Probable antibiotic-resistance protein Rv2303c, (MTCY339.06, MT2360), len: 307 aa. Probable antibiotic-resistance protein, with some similarity to Q54229|G153373 macrotetrolide antibiotic-resistance protein (NONR) from Streptomyces griseus (347 aa) (see Plater and Robinson, 1992), FASTA scores: opt: 438, E(): 3.1e-21, (33.2% identity in 226 aa overlap); and other hypothetical proteins e.g. P95886 ORF C02006 from Sulfolobus solfataricus (269 aa), FASTA scores: opt: 252, E(): 3.5e-09, (25.5% identity in 286 aa overlap); etc. Also similar to Mycobacterium tuberculosis Rv3510c|O53555|MTV023.17. Note that the protein Q9XDF3|NONC from Streptomyces griseus subsp. griseus (317 aa) is equivalent to Q54229|G153373|NONR however the N-terminal end is shorter (30 aa) owing to a changed start codon (see Walczak et al., 2000). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q50662 No virulence, detoxification, adaptation Q50662 Q50662 GO:0008152,GO:0003824 Q50662 NC_000962.3 Mycobrowser_v5 CDS 2577108 2577701 . + 0 Rv2306A Rv2306A Unknown Possible conserved membrane protein Rv2306A, len: 197 aa. Possible conserved membrane protein, similar to several hypothetical membrane proteins from Mycobacterium tuberculosis and Streptomyces coelicolor, e.g. Rv0625c|P96915|Y625_MYCTU hypothetical 25.2 KDA protein from Mycobacterium tuberculosis (246 aa), FASTA scores: opt: 410, E(): 2.7e-17, (53.25% identity in 139 aa overlap). First 140 aa show high similarity, this then decreases but continues in next ORF Rv2306B, suggesting a frameshift near nt 2577473. However the sequence has been checked and no error found. The sequence is identical in CDC1551 and Mycobacterium bovis. Replaces original Rv2306c on other strand. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q79FG5 No cell wall and cell processes Q79FG5 NC_000962.3 Mycobrowser_v5 CDS 2583435 2583779 . + 0 Rv2310 Rv2310 Use for sequence excision. Possible excisionase Rv2310, (MT2372, MTCY3G12.24c), len: 114 aa. Possible excisionase, showing some similarity to others e.g. Q9LCU5 putative excisionase from Arthrobacter sp. TM1 (174 aa) FASTA scores: opt: 341, E(): 6.6e-15, (48.2% identity in 110 aa overlap); O85865 putative excisionase from Sphingomonas aromaticivorans (152 aa), FASTA scores: opt: 205, E(): 2.2e-06, (41.25% identity in 80 aa overlap); etc. Also similar to Rv3750c|O69717 hypothetical protein from Mycobacterium tuberculosis (130 aa), FASTA scores: opt: 228, E(): 6.9e-08, (43.9% identity in 82 aa overlap). Contains possible helix-turn-helix motif at aa 20-41 (Score 2181, +6.62 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WLC3 No insertion seqs and phages P9WLC3 GO:0000166,GO:0003677 NC_000962.3 Mycobrowser_v5 CDS 2587287 2588804 . - 0 Rv2315c Rv2315c Function unknown Conserved protein Rv2315c, (MTCY3G12.19), len: 505 aa. Conserved protein, highly similar to Q9S273|SCI28.10 hypothetical 47.1 KDA protein from Streptomyces coelicolor (435 aa), FASTA scores: opt: 1768, E():5.6e-101, (63.2% identity in 432 overlap); and similar to others e.g. AAK24787|CC2823 hypothetical protein (TldD/PmbA family) from Caulobacter crescentus (543 aa), FASTA scores: opt: 876, E():3.1e-46, (42.8% identity in 505 overlap); O58578|PH0848 hypothetical 54.4 KDA protein from Pyrococcus horikoshii (481 aa), FASTA scores: opt: 661, E(): 4.3e-33, (29.95% identity in 484 aa overlap); Q9UZ95|PAB1547 hypothetical 53.6 KDA protein from Pyrococcus abyssi (473 aa), FASTA scores: opt: 656, E(): 8.6e-33, (29.1% identity in 481 aa overlap); etc. P71897 No conserved hypotheticals P71897 P71897 NC_000962.3 Mycobrowser_v5 CDS 2588838 2589710 . + 0 Rv2316 uspA Thought to be involved in active transport of sugar across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable sugar-transport integral membrane protein ABC transporter UspA Rv2316, (MTCY3G12.18c), len: 290 aa. Probable uspA, sugar-transport integral membrane protein ABC transporter (see citation below), most similar to Q9CBN8|USPA|ML1768 sugar transport integral membrane protein from Mycobacterium leprae (328 aa), FASTA scores: opt: 1593, E(): 1.9e-93, (82.35% identity in 289 aa overlap); and similar to O32940|ML1426|MLCB2052.28 possible sugar transport protein (probable ABC-transport protein, inner membrane component) from Mycobacterium leprae (319 aa), FASTA scores: opt: 600, E(): 9.2e-31, (34.25% identity in 295 aa overlap). Also similar to other proteins involved in transport e.g. Q9X860|SCE134.05c putative binding protein dependent transport protein from Streptomyces coelicolor (327 aa), FASTA scores: opt: 639, E(): 3.2e-33, (40.45% identity in 272 aa overlap); Q9K6N9|BH3689 sugar transport system (permease) from Bacillus halodurans (300 aa), FASTA scores: opt: 590, E(): 3.7e-30, (35.65% identity in 289 aa overlap); etc. P71896 No cell wall and cell processes P71896 P71896 GO:0005886,GO:0006810,GO:0005215,GO:0016021 P71896 NC_000962.3 Mycobrowser_v5 CDS 2594154 2594699 . - 0 Rv2321c rocD2 Involved in arginine metabolism [catalytic activity: L-ornithine + a 2-oxo acid = L-glutamate 5-semialdehyde + an L-amino acid]. Probable ornithine aminotransferase (C-terminus part) RocD2 (ornithine--oxo-acid aminotransferase) Rv2321c, (MTCY3G12.13), len: 181 aa. Probable rocD2, ornithine aminotransferase, highly similar to C-terminal region of other ornithine aminotransferases, e.g. Q9FC90|ROCD from Streptomyces coelicolor (407 aa), FASTA scores: opt: 628, E(): 1.2e-32, (55.35% identity in 168 aa overlap); P3802|OAT_BACSU|ROCD from Bacillus subtilis (401 aa), FASTA scores: opt: 477, E(): 4.3e-23, (42.1% identity in 178 aa overlap); BAB42057|ROCD|SA0818 from Staphylococcus aureus subsp. aureus N315 (396 aa), FASTA scores: opt: 437, E(): 1.5e-20, (41.3% identity in 170 aa overlap); etc. Contains PS00600 Aminotransferases class-III pyridoxal-phosphate attachment site. Belongs to class-III of pyridoxal-phosphate-dependent aminotransferases. Rv2322c|MTCY3G12.12 (upstream ORF) and Rv2321c|MTCY3G12.13 appear to be an ornithine aminotransferase homologue but are frameshifted - we can find no sequence error in the cosmid to account for this. P71891 No intermediary metabolism and respiration P71891 P71891 GO:0030170,GO:0004587 2.6.1.13 P71891 NC_000962.3 Mycobrowser_v5 CDS 2597009 2597857 . - 0 Rv2325c Rv2325c Function unknown Conserved hypothetical protein Rv2325c, (MTCY3G12.09), len: 282 aa. Conserved hypothetical protein, equivalent to O32970|MLCB22.37c|ML0849 hypothetical protein from Mycobacterium leprae (283 aa), FASTA scores: opt: 1405, E(): 1.8e-78, (77.7% identity in 282 aa overlap). Also some similarity to other proteins e.g. Q9Z9J1|YBAF|BH0166 YBAF protein (BH0166 protein) (hypothetical protein) from Bacillus halodurans (265 aa), FASTA scores: opt: 288, E(): 2.8e-10, (25.8% identity in 264 aa overlap); P70972|YBAF YBAF protein (hypothetical protein) from Bacillus subtilis (265 aa), FASTA scores: opt: 259, E(): 1.5e-08, (25.45% identity in 224 aa overlap); AAK34821|SPY2193|Q99X13 Conserved hypothetical protein from Streptococcus pyogenes (266 aa), FASTA scores: opt: 232, E(): 6.5e-07, (25.1% identity in 267 aa overlap); etc. P9WPI7 No conserved hypotheticals P64997 P9WPI7 GO:0015087,GO:0006824,GO:0016021,GO:0005886,GO:0009236 NC_000962.3 Mycobrowser_v5 CDS 2644320 2645774 . + 0 Rv2363 amiA2 Generates monocarboxylate from monocarboxylic acid amide [catalytic activity: a monocarboxylic acid amide + H(2)O = a monocarboxylate + NH(3)]. Probable amidase AmiA2 (aminohydrolase) Rv2363, (MTCY27.17c), len: 484 aa. Probable amiA2, amidase, highly similar or similar to others e.g. O28325|YJ54_ARCFU|AF1954 putative amidase from Archaeoglobus fulgidus (453 aa), FASTA scores: opt: 777, E(): 1.1e-38, (35.0% identity in 474 aa overlap); Q55424|AMID_SYNY3|SLL0828 putative amidase from Synechocystis sp. strain PCC 6803 (506 aa), FASTA scores: opt: 770, E(): 3e-38, (36.4% identity in 456 aa overlap); Q53116|AMDA enantiomerase-selective amidase from Rhodococcus sp. (462 aa), FASTA scores: opt: 701, E(): 3.5e-34, (32.7% identity in 468 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. AMI2_MYCTU|AMIB2|Q11056|Rv1263|MT1301|MTCY50.19c|cy50.19c amidase (462 aa), FASTA scores: opt: 1141, E(): 2.9e-60, (45.4% identity in 454 aa overlap); etc. Contains PS00571 Amidases signature, and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the amidase family. P9WQ99 No intermediary metabolism and respiration P63490 P9WQ99 GO:0016884,GO:0004040 3.5.1.4 P9WQ99 NC_000962.3 Mycobrowser_v5 CDS 2597854 2599947 . - 0 Rv2326c Rv2326c Probably involved in active transport accross the membrane. Thought to be responsible for energy coupling to the transport system and the translocation of the substrate across the membrane. Possible transmembrane ATP-binding protein ABC transporter Rv2326c, (MTC3G12.08), len: 697 aa. Possible transmembrane ATP-binding protein ABC transporter (see citation below). Equivalent to Q9CCF9|ML0848 ABC transporter from Mycobacterium leprae (724 aa), FASTA scores: opt: 3482, E(): 2.8e-182, (76.9% identity in 697 aa overlap) and also to O32971|MLCB22.38c ABC-type transporter from Mycobacterium leprae (726 aa), FASTA scores: opt: 3482, E(): 2.8e-182, (76.9% identity in 697 aa overlap). Similar in part to other ABC transporters e.g. Q9WY65|TM0222 from Thermotoga maritima (266 aa), FASTA scores: opt: 407, E(): 4.2e-15, (38.0% identity in 213 aa overlap); etc. Contains 2 X PS00017 ATP/GTP-binding site motif A (P-loop); and 2 x PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). P9WQI7 No cell wall and cell processes P63399 P9WQI7 GO:0016887,GO:0016021,GO:0005886,GO:0006810,GO:0005524 P9WQI7 NC_000962.3 Mycobrowser_v5 CDS 2605108 2606754 . + 0 Rv2332 mez Catalyzes the oxidative decarboxylation of malate into pyruvate, important for a wide range of metabolic pathways [catalytic activity: (S)-malate + NAD(+) = pyruvate + CO(2) + NADH]. Probable [NAD] dependent malate oxidoreductase Mez (malic enzyme) (NAD-malic enzyme) (malate dehydrogenase (oxaloacetate decarboxylating)) (pyruvic-malic carboxylase) (NAD-me) Rv2332, (MTCY3G12.02c, MTCY98.01, MT2394), len: 548 aa. Probable mez, malate oxidoreductase [NAD] dependent (malic enzyme), highly similar to others e.g. O34389|MALS putative malolactic enzyme [includes: malic enzyme ; L-lactate dehydrogenase] from Bacillus subtilis (566 aa), FASTA scores: opt: 1927, E(): 5.5e-111, (52.9% identity in 539 aa overlap); P45868|MAO2_BACSU|YWKA probable NAD-dependent malic enzyme from Bacillus subtilis (582 aa), FASTA scores: opt: 1849, E(): 3.6e-106, (50.45% identity in 543 aa overlap); Q48796|MLES_OENOE malolactic enzyme from Oenococcus oeni (541 aa), FASTA scores: opt: 1540, E(): 3.6e-87, (44.2% identity in 536 aa overlap); etc. Belongs to the malic enzymes family. N-terminus shortened since first submission (previously 652 aa). P9WK25 No intermediary metabolism and respiration P71880 P9WK25 GO:0016619,GO:0006108,GO:0046872,GO:0055114,GO:0051287 1.1.1.38 P9WK25 NC_000962.3 Mycobrowser_v5 CDS 2608796 2609728 . + 0 Rv2334 cysK1 Involved in cysteine biosynthesis [catalytic activity: O3-acetyl-L-serine + H(2)S = L-cysteine + acetate]. Cysteine synthase a CysK1 (O-acetylserine sulfhydrylase A) (O-acetylserine (thiol)-lyase A) (CSASE A) Rv2334, (MT2397, MTCY98.03), len: 310 aa. cysK1, cysteine synthase A, equivalent to O32978|CYSK_MYCLE|ML0839|MLCB22.47 cysteine synthase a from Mycobacterium leprae (310 aa), FASTA scores: opt: 1756, E(): 8.6e-96, (85.8% identity in 310 aa overlap). Also highly similar to other cysteine synthases e.g. Q9JQL6|CYSK|NMA0974|NMB0763 putative cysteine synthase from Neisseria meningitidis (serogroup a and B) (310 aa), FASTA scores: opt: 1368, E(): 4.6e-73, (66.45% identity in 310 aa overlap); P73410|CYSK_SYNY3|SLR1842 from Synechocystis sp (312 aa), FASTA scores: opt: 1310, E(): 1.2e-69, (64.65% identity in 311 aa overlap); Q43725|CYSM_ARATH|OASC|ACS1|AT3G59760|F24G16.30 cysteine synthase (mitochondrial precursor) from Arabidopsis thaliana (Mouse-ear cress) (424 aa), FASTA scores: opt: 1253, E(): 3.2e-66, (59.2% identity in 309 aa overlap) (has its N-terminus longer 104 aa); etc. Contains PS00901 Cysteine synthase/cystathionine beta-synthase P-phosphate attachment site. Belongs to the cysteine synthase/cystathionine beta-synthase family. Note that previously known as cysK. P9WP55 No intermediary metabolism and respiration 2Q3B,2Q3C,2Q3D P0A534 P9WP55 GO:0004124,GO:0030170,GO:0016740,GO:0006535 2.5.1.47 P9WP55 NC_000962.3 Mycobrowser_v5 CDS 2613107 2614063 . - 0 Rv2338c moeW Involved in molybdoptenum cofactor biosynthesis; thought to be involved in the biosynthesis of a demolybdo-cofactor (molybdopterin), necessary for molybdo-enzymes. Possible molybdopterin biosynthesis protein MoeW Rv2338c, (MTCY98.07c), len: 318 aa. Possible moeW, molybdoptenum biosynthesis protein, showing some similarity to several molybdopterin biosynthesis proteins e.g. O27613|MTH1571 molybdopterin biosynthesis protein MOEB homolog from Methanobacterium thermoautotrophicum (251 aa), FASTA scores: opt: 309, E(): 4.7e-14; (30.7% identity in 254 aa overlap); Q9KPQ5|VC2311 HESA/MOEB/THIF family protein from Vibrio cholerae (273 aa), FASTA scores: opt: 255, E(): 4e-09, (36.25% identity in 149 aa overlap); Q9PD34|XF1545 molybdopterin biosynthesis protein from Xylella fastidiosa (276 aa), FASTA scores: opt: 233,E(): 1e-07, (33.6% identity in 128 aa overlap); etc. Seems to belong to the HESA/MOEB/THIF family. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P95234 No intermediary metabolism and respiration P95234 P95234 GO:0003824,GO:0008152 P95234 NC_000962.3 Mycobrowser_v5 CDS 2620533 2622452 . - 0 Rv2343c dnaG DNA primase is the polymerase that synthesizes small RNA primers for the okazaki fragments on both template strands replication FORKS during chromosomal DNA synthesis. Probable DNA primase DnaG Rv2343c, (MTCY98.12c), len: 639 aa. Probable dnaG, DNA primase, equivalent to O52200|PRIM_MYCSM|DNAG DNA primase from Mycobacterium smegmatis (636 aa), FASTA scores: opt: 3504, E(): 5.5e-202, (81.55% identity in 639 aa overlap); and Q9CCG2|DNAG|ML0833 DNA primase from Mycobacterium leprae (642 aa), FASTA scores: opt: 3443, E(): 2.5e-198, (80.4% identity in 642 aa overlap). Also highly similar to many DNA primases e.g. Q9S1N4|PRIM_STRCO|DNAG|SC7A8.07c from Streptomyces coelicolor (641 aa), FASTA scores: opt: 1899, E(): 5.1e-106, (47.9% identity in 643 aa overlap); P74893|PRIM_SYNP7|DNAG from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (616 aa), FASTA scores: opt: 860, E(): 6.6e-44, (35.3% identity in 513 aa overlap); P05096|PRIM_BACSU from Bacillus subtilis (603 aa) FASTA scores: opt: 800, E(): 2.5e-40, (33.7% identity in 430 aa overlap); etc. P9WNW1 No information pathways P63962 P9WNW1 GO:0003896,GO:0006269,GO:0005658,GO:0006350,GO:0008270,GO:0003677 2.7.7.- P9WNW1 NC_000962.3 Mycobrowser_v5 CDS 2627172 2628698 . - 0 Rv2349c plcC Hydrolyzes sphingomyelin in addition to phosphatidylcholine. Probable virulence factor implicated in the pathogenesis of Mycobacterium tuberculosis at the level of intracellular survival, by the alteration of cell signaling events or by direct cytotoxicity [catalytic activity: a phosphatidylcholine + H(2)O = 1,2- diacylglycerol + choline phosphate]. Probable phospholipase C 3 PlcC Rv2349c, (MT2414, MTCY98.18c), len: 508 aa. Probable plcC, phospolipase C 3 (see citations below), similar to other precursors of several phospolipases C e.g. P15713|PHLN_PSEAE|PA3319 non-hemolytic phospholipase C precursor from Pseudomonas aeruginosa (692 aa), FASTA scores: opt: 1013, E(): 9.3e-54, (38.85% identity in 525 aa overlap); P06200|PHLC_PSEAE hemolytic phospholipase C precursor from Pseudomonas aeruginosa (730 aa), FASTA scores: opt: 630, E(): 1.5e-30, (35.15% identity in 535 aa overlap); Q9S816|T12J13.18|T21P5.4 putative phospholipase from Arabidopsis thaliana (Mouse-ear cress) (521 aa), FASTA scores: opt: 218, E(): 1e-05, (27.05% identity in 451 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. Q9XB13|PLCD|Rv1755c|MT1799|MTCY28.21C phospholipase C 4 (514 aa), FASTA scores: opt: 2497, E(): 9e-144, (68.35% identity in 509 aa overlap); Q50560|Rv2351c|PLCA|MTP40|MT2416|MTCY98.20c phospholipase C 1 (520 aa), FASTA scores: opt: 2494, E(): 1.4e-143, (68.1% identity in 514 aa overlap); P95246|PLCB|MPCB|Rv2350c|MT2415|MTCY98.19c phospholipase C 2 (512 aa), FASTA scores: opt: 2474, E(): 2.2e-142, (67.65% identity in 513 aa overlap); etc. Belongs to the bacterial phospholipase C family. P9WIB1 No intermediary metabolism and respiration P95245 P9WIB1 GO:0034480 3.1.4.3 P9WIB1 NC_000962.3 Mycobrowser_v5 CDS 2634528 2635592 . - 0 Rv2353c PPE39 Function unknown PPE family protein PPE39 Rv2353c, (MTCY98.22c), len: 354 aa. PPE39, Member of Mycobacterium tuberculosis PPE family, highly similar to many e.g. near ORF P95249|Rv2356c|MTCY98.25 from Mycobacterium tuberculosis (615 aa), FASTA scores: opt: 1566, E(): 3.2e-69, (66.1% identity in 349 aa overlap); Q10778|MTCY48.17, Q10540|MTCY31.06c, E241779|MTCY98, Q10813|MTCY274.23c, P71868|MTCY03C7.23, P71869|MTCY03C7.24c, P42611|MTV037.06C, E64997|MTCY98, Q10707|MTCY49.38C, P71657|MTCY02B10.25c, etc. Note that the ATG and RBS appear to be provided by the IR of neighbouring IS6110. Predicted to be an outer membrane protein (See Song et al., 2008). Q79FF3 No PE/PPE Q79FF3 Q79FF3 NC_000962.3 Mycobrowser_v5 CDS 2639673 2641064 . - 0 Rv2357c glyS Involved in translation mechanisms [catalytic activity: ATP + L-glycine + tRNA(GLY) = AMP + pyrophosphate + L-glycyl-tRNA(GLY)]. Probable glycyl-tRNA synthetase GlyS (glycine--tRNA ligase) (GLYRS) Rv2357c, (MTCY27.23, MTCY98.26), len: 463 aa. Probable glyS, glycyl-tRNA synthetase, equivalent to Q9CCG4|GLYS|ML0826 putative glycyl-tRNA synthase from Mycobacterium leprae (463 aa), FASTA scores: opt: 2898, E(): 1e-179, (90.2% identity in 459 aa overlap). Also highly similar to others e.g. Q9L2H9|SYG_STRCO|SCC121.07c from Streptomyces coelicolor (460 aa), FASTA scores: opt: 2210, E(): 2.9e-135, (68.3% identity in 457 aa overlap); Q9PPZ7|SYG_UREPA|GLYS|UU493 glycyl-tRNA synthetase from Ureaplasma parvum (Ureaplasma urealyticum biotype 1) (473 aa), FASTA scores: opt: 1254, E(): 1.7e-73, (45.25% identity in 462 aa overlap); P75425|SYG_MYCPN|GLYS|MPN354|MP482 glycyl-tRNA synthetase from Mycoplasma pneumoniae (449 aa), FASTA scores: opt: 1074, E(): 6.9e-62, (39.45% identity in 454 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00179 Aminoacyl-transfer RNA synthetases class-II signature 1. Belongs to class-II aminoacyl-tRNA synthetase family. P9WFV7 No information pathways P67032 P9WFV7 GO:0005737,GO:0004820,GO:0006426,GO:0046983,GO:0005524 6.1.1.14 P9WFV7 NC_000962.3 Mycobrowser_v5 CDS 2643461 2644258 . - 0 Rv2362c recO Involved in DNA repair and RECF pathway recombination Possible DNA repair protein RecO Rv2362c, (MTCY27.18), len: 265 aa. RecO, DNA repair protein, equivalent to Q9CCN0|ML0633 Mycobacterium leprae Hypothetical protein (268 aa), FASTA scores: opt: 1560, E(): 8.5e-93, (86.6% identity in 268 aa overlap). Also highly similar to others e.g. Q9L2H3|SCC121.13c DNA repair protein recO from Streptomyces coelicolor (251 aa), FASTA scores: opt: 843, E(): 6.9e-47, (52.2% identity in 249 aa overlap); and similar to other hypothetical proteins. Weak similarity with P42095|RECO_BACSU DNA repair protein recombinase from Bacillus subtilis (255 aa), FASTA scores: opt: 270, E(): 3.6e-10, (26.4% identity in 182 aa overlap). Maybe involved in modulating assembly and disassembly of RECA filaments (with RECF|Rv0003 and RECR|Rv3715c) (see citation below). Contains match to Pfam entry PF02565 Recombination protein O. Belongs to the RECO family. P9WHI5 No information pathways P65983 P9WHI5 GO:0006281,GO:0006310 P9WHI5 NC_000962.3 Mycobrowser_v5 CDS 2648916 2649974 . - 0 Rv2368c phoH1 Unknown Probable PHOH-like protein PhoH1 (phosphate starvation-inducible protein PSIH) Rv2368c, (MTCY27.12), len: 352 aa. Probable phoH1, phoH-like protein (phosphate starvation-induced protein), probably ATP-binding protein, equivalent to Q49751|PHOL_MYCLE| ML0627|B1937_F1_20 PHOH-like protein from Mycobacterium leprae (349 aa), FASTA scores: opt: 1952, E(): 4.7e-107, (88.9% identity in 352 aa overlap). Also highly similar to Q9L2L5|SCC117.05 PHOH-like protein from Streptomyces coelicolor (359 aa), FASTA scores: opt: 1407, E(): 3.6e-75, (63.6% identity in 349 aa overlap); Q9RSY1|DR1988 PHOH-related protein from Deinococcus radiodurans (380 aa), FASTA scores: opt: 1053, E(): 1.9e-54, (53.3% identity in 349 aa overlap); Q9KD58|PHOH|BH1361 phosphate starvation-induced protein from Bacillus halodurans (320 aa), FASTA scores: opt: 1019, E(): 1.6e-52, (54.35% identity in 300 aa overlap); P46343|PHOL_BACSU PHOH-like protein from Bacillus subtilis (319 aa), FASTA scores: opt: 1014, E(): 3.2e-52, (50.8% identity in 303 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the PHOH family. Note that previously known as phoH. P9WIA3 No intermediary metabolism and respiration P0A5S0 P9WIA3 GO:0003723,GO:0005737,GO:0005524 P9WIA3 NC_000962.3 Mycobrowser_v5 CDS 2652037 2652825 . - 0 Rv2372c Rv2372c Function unknown Conserved hypothetical protein Rv2372c, (MTCY27.08), len: 262 aa. Conserved hypothetical protein, equivalent to Q9CCN1|ML0626 hypothetical protein from Mycobacterium leprae (257 aa), FASTA scores: opt: 1277, E(): 3e-71, (77.25% identity in 255 aa overlap). Also highly similar to others e.g. Q9RDD9|SDRD hypothetical 26.1 KDA protein from Streptomyces coelicolor (249 aa), FASTA scores: opt: 624, E(): 3.2e-31, (45.05% identity in 253 aa overlap); P54461|YQEU_BACSU hypothetical 28.8 kDa protein from Bacillus subtilis (256 aa), FASTA scores: opt: 375, E(): 6e-16, (32.5% identity in 234 aa overlap); etc. C-terminal half highly similar to Q49763|B1937_F2_57 from Mycobacterium leprae (128 aa), FASTA scores: opt: 577, E(): 1.4e-28, (75.8% identity in 124 aa overlap). Belongs to the UPF0088 family. P9WGX1 No conserved hypotheticals P67202 P9WGX1 GO:0008168,GO:0006364,GO:0005737 2.1.1.- P9WGX1 NC_000962.3 Mycobrowser_v5 CDS 2654062 2655093 . - 0 Rv2374c hrcA Involved in transcriptional regulation (repression) of class I heat shock proteins e.g. DNAK-GRPE-DNAJ1 and groels operons). Prevents heat-shock induction of these operons. Probable heat shock protein transcriptional repressor HrcA Rv2374c, (MTCY27.06), len: 343 aa. Probable hrcA, heat-inducible transcriptional repressor (see citation below), equivalent to Q9CCN2|HRCA|ML0624 putative heat-inducible transcriptional regulator from Mycobacterium leprae (343 aa), FASTA scores: opt: 1926, E(): 3.9e-107, (89.8% identity in 343 aa overlap). Also highly similar to other heat-inducible transcription repressor proteins e.g. Q9RDD6|HRCA|SCC77.22c from Streptomyces coelicolor (338 aa), FASTA scores: opt: 1227, E(): 1.1e-65, (58.8% identity in 335 aa overlap); O52163|HRCA_STRAL from Streptomyces albus (338 aa), FASTA scores: opt: 1196, E(): 7.7e-64, (56.1% identity in 335 aa overlap); P25499|HRCA_BACSU heat-inducible transcription repressor from Bacillus subtilis (343 aa), FASTA scores: opt: 538, E(): 8.4e-25, (28.9% identity in 325 aa overlap); etc. Almost identical, but conflict at C-terminus, to Q49749|YGRP|B1937_F1_18 putative heat-inducible transcription repressor from Mycobacterium leprae (197 aa) FASTA scores: opt: 1126, E(): 6.9e-60, (91.8% identity in 195 aa overlap). Belongs to the HRCA family. P9WMK3 No virulence, detoxification, adaptation P64398 P9WMK3 GO:0045892,GO:0006950,GO:0006350,GO:0016564,GO:0003677 P9WMK3 NC_000962.3 Mycobrowser_v5 CDS 2656215 2656430 . - 0 Rv2377c mbtH Thought to be involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins. Putative conserved protein MbtH Rv2377c, (MT2445.1, MTCY27.03), len: 71 aa. Putative mbtH, conserved protein with no function assigned (see Quadri et al., 1998; De Voss et al., 1999), similar to hypothetical proteins or proteins found in several gene clusters for biosynthesis or transport of siderophores and other nonribosomally synthesized peptides e.g. Q9Z388|SCE8.11c putative small conserved hypothetical protein from Streptomyces coelicolor (71 aa), FASTA scores: opt: 345, E(): 1.4e-19, (68.2% identity in 66 aa overlap); Q9F8V3|CUMB COUY protein (probably involved in the biosynthesis of aminocoumarin antibiotic coumermycin a(1)) (see Wang et al., 2000) from Streptomyces rishiriensis (71 aa), FASTA scores: opt: 329, E(): 2.2e-18, (63.2% identity in 68 aa overlap); Q9F5J2|SIM-CB MBTH-like protein (probably protein involved in the biosynthesis of aminocoumarin antibiotic coumermycin a(1)) from Streptomyces antibioticus (70 aa), FASTA scores: opt: 308, E(): 8.4e-17, (65.6% identity in 64 aa overlap); Q9FB14 MBTH-like protein (involved in the biosynthesis of the antitumor drug bleomycin) (see Du et al., 2000) from Streptomyces verticillus FASTA scores: opt: 220, E(): 8.8e-10, (41.2% identity in 68 aa overlap); etc. P9WIP5 No lipid metabolism 2KHR O05821 P9WIP5 P9WIP5 NC_000962.3 Mycobrowser_v5 CDS 2656408 2657703 . - 0 Rv2378c mbtG Involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins. This hydroxylase is possibly required for N-hydroxylation of the two lysine residues at some stage during mycobactin assembly [catalytic activity: L-lysine + O(2) = N6-hydroxy-L-lysine + H(2)O. No information can be found if this enzyme is NADPH dependent or independent]. Lysine-N-oxygenase MbtG (L-lysine 6-monooxygenase) (lysine N6-hydroxylase) Rv2378c, (MTCY27.02), len: 431 aa. MbtG, lysine-N-oxygenase (hydroxylase) (EC 1.13.12.10 or 1.14.13.59; depending if enzyme is NADPH dependent or independent) (see citations below), showing some similarity with various proteins including ornithine and lysine-N-oxygenases, e.g. Q9K6Q1|TRKA|BH3677 potassium uptake protein from Bacillus halodurans (350 aa), FASTA scores: opt: 153, E(): 0.016, (25.2% identity in 246 aa overlap); P56584|SID1_USTMA L-ornithine 5-monooxygenase from Ustilago maydis (Smut fungus) (570 aa), FASTA scores: opt: 136, E(): 0.31, (22.85% identity in 127 aa overlap); Q9HHV0|HXYA|VNG6214G monooxygenase from Halobacterium sp. strain NRC-1 (477 aa), FASTA scores: opt: 119, E(): 3.4, (40.0% identity in 70 aa overlap); O69828|SC1A6.23 putative lysine N-hydroxlase (fragment) from Streptomyces coelicolor (134 aa), blast score: 76 (similarity in part for this one); etc. Cofactors: FAD (by similarity). P9WKF7 No lipid metabolism P9WKF7 GO:0006826,GO:0055114,GO:0047091 1.14.13.59 P9WKF7 NC_000962.3 Mycobrowser_v5 CDS 2720776 2721777 . - 0 Rv2424c Rv2424c Required for the transposition of the insertion element IS1558. Probable transposase Rv2424c, (MTCY428.23), len: 333 aa. Probable transposase for IS1558, similar to is element proteins e.g. AL021957|Rv2177c|MTV021_10 from Mycobacterium tuberculosis (221 aa), FASTA scores: opt: 1491, E(): 6.2e-87, (98.6% identity in 221 aa overlap); P19780|YIS1_STRCO hypothetical insertion element IS110 from Streptomyces coelicolor (45 aa), FASTA scores: opt: 203, E(): 1.7e-05; (27.3% identity in 238 aa overlap); etc. Contains PS01159 WW/rsp5/WWP domain signature. P71924 No insertion seqs and phages P71924 P71924 GO:0004803,GO:0006313,GO:0003677 P71924 NC_000962.3 Mycobrowser_v5 CDS 2667255 2670269 . - 0 Rv2381c mbtD Involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins. Polyketide synthetase MbtD (polyketide synthase) Rv2381c, (MTCY22H8.04), len: 1004 aa. MbtD, polyketide synthase (see citations below), similar in part to several synthases e.g. Q03132|ERY2_SACER|ERYA erythronolide synthase, modules 3 and 4 from Saccharopolyspora erythraea (Streptomyces erythraeus) (3567 aa), FASTA scores: opt: 971, E(): 1e-46, (29.35% identity in 1043 aa overlap); Q9F829|megaii megalomicin 6-deoxyerythronolide B synthase 2 from Micromonospora megalomicea subsp. nigra (3562 aa), FASTA scores: opt: 787, E(): 2.4e-36, (29.35% identity in 1032 aa overlap); Q9L4W4|NYSB polyketide synthase from Streptomyces noursei (3192 aa), FASTA scores: opt: 761, E(): 6.6e-35, (29.55% identity in 1086 aa overlap); O30764|NIDA1 polyketide synthase modules 1 and 2 from Streptomyces caelestis (4340 aa), FASTA scores: opt: 726, E(): 7.8e-33, (27.3% identity in 1052 aa overlap); etc. Contains PS00012 Phosphopantetheine attachment site. P71719 No lipid metabolism P71719 P71719 GO:0009058,GO:0048037,GO:0031177,GO:0016740,GO:0000036 P71719 NC_000962.3 Mycobrowser_v5 CDS 2983071 2983874 . + 0 Rv2666 Rv2666 Required for the transposition of the insertion element IS1081. Probable transposase for insertion sequence element IS1081 (fragment) Rv2666, (MTCY441.35), len: 267 aa. Probable transposase (fragment), identical in region of overlap to P35882|TRA1_MYCBO|TRA1_MYCTU transposase for insertion sequence element IS1081 from Mycobacterium tuberculosis or bovis (415 aa). Last 4 codons not part of gene. Contains PS01007 Transposases, Mutator family, signature. P71963 No insertion seqs and phages P71963 P71963 GO:0004803,GO:0006313,GO:0003677 P71963 NC_000962.3 Mycobrowser_v5 CDS 2677729 2678649 . + 0 Rv2385 mbtJ Involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins. Possibly required for N-hydroxylation of the two lysine residues at some stage during mycobactin assembly. Putative acetyl hydrolase MbtJ Rv2385, (MTCY253.36c), len: 306 aa. Putative mbtJ, acetyl hydrolase (see citations below), showing some similarity with various hydrolases including acetyl hydrolases e.g. Q9ZBM4|MLCB1450.08|ML0314 putative hydrolase/esterase from Mycobacterium leprae (335 aa), FASTA scores: opt: 449, E(): 6.7e-21, (33.85% identity in 313 aa overlap); AAK47950|MT3591 Esterase from M. tuberculosis strain CDC1551 (327 aa), FASTA scores: opt: 469, E(): 3.6e-22, (35% identity in 283 aa overlap); Q9X8J4|SCE9.22 putative esterase from Streptomyces coelicolor (266 aa), FASTA scores: opt: 430,E(): 8.5e-20, (38% identity in 245 aa overlap); Q01109|BAH_STRHY acetyl-hydrolase from Streptomyces hygroscopicus (299 aa), FASTA scores: opt: 420, E(): 4e-19, (35.1% identity in 265 aa overlap). Equivalent to AAK46748 from Mycobacterium tuberculosis strain CDC1551 (327 aa) but shorter 21 aa. Note that previously known as lipK. Q79FE8 No lipid metabolism Q79FE8 Q79FE8 GO:0016787 3.1.1.- Q79FE8 NC_000962.3 Mycobrowser_v5 CDS 2678653 2680005 . - 0 Rv2386c mbtI Involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins. Possibly plays a role in the conversion of chorismate to salicilate (the starter unit for mycobactin siderophore construction). Isochorismate synthase MbtI Rv2386c, (MTCY253.35), len: 450 aa. mbtI, isochorismate synthase (see citations below), similar to Q9X9I8|IRP9 salicylate synthetase from Yersinia enterocolitica (434 aa), FASTA scores: opt: 887, E(): 7.5e-48, (37.45% identity in 422 aa overlap); and similar in C-terminal region to many anthranilate synthases component I e.g. Q9Z4W7|TRPE_STRCO|SCE8.07c from Streptomyces coelicolor (511 aa), FASTA scores: opt: 509, E(): 3e-24, (40.4% identity in 255 aa overlap); P33975|TRPE_HALVO from Halobacterium volcanii (Haloferax volcanii) (523 aa) FASTA scores: opt: 488, E(): 6.2e-23, (34.2% identity in 298 aa overlap); and similar to Q08653|TRPE_THEMA|TM0142 anthranilate synthase component I from Thermotoga maritima (461 aa), FASTA scores: opt: 478, E(): 2.3e-22, (28.4% identity in 440 aa overlap); etc. Could be belong to the anthranilate synthase component I family. Note that previously known as trpE2, an anthranilate synthase component I. P9WFX1 No lipid metabolism 2G5F,2I6Y,3LOG Q7D785 P9WFX1 GO:0006826,GO:0008909,GO:0016829,GO:0046872,GO:0009058 4.1.3.-,5.4.4.2 P9WFX1 NC_000962.3 Mycobrowser_v5 CDS 2690072 2692075 . + 0 Rv2395 Rv2395 Function unknown (possibly involved in transport across the membrane). Probable conserved integral membrane protein Rv2395, (MTCY253.26c), len: 667 aa. Probable conserved integral membrane protein, similar to AAK24613|CC2646 oligopeptide transporter/opt family protein from Caulobacter crescentus (666 aa), FASTA scores: opt: 1638, E(): 4.8e-86, (51.0% identity in 658 aa overlap); Q9PIS5|CJ0204 putative integral membrane protein from Campylobacter jejuni (665 aa), FASTA scores: opt: 1484, E(): 2.9e-77, (40.6% identity in 658 aa overlap); and P44016|Y561_HAEIN hypothetical integral membrane protein from Haemophilus influenzae (635 aa), FASTA scores: opt: 1449, E(): 2.8e-75, (42.15% identity in 624 aa overlap). P71749 No cell wall and cell processes P71749 P71749 GO:0055085 P71749 NC_000962.3 Mycobrowser_v5 CDS 2696644 2697714 . - 0 Rv2400c subI Involved in the active transport across the membrane of multiple sulfur-containing compounds, including sulfate and thiosulfate (import). Probable sulfate-binding lipoprotein SubI Rv2400c, (MTCY253.21), len: 356 aa. Probable subI, sulfate-binding lipoprotein component of sulfate transport system (see citations below), equivalent to Q9CCN3|SUBI|ML0615 (alias Q49748|B1937_F1_11, 358 aa) putative sulphate-binding protein from Mycobacterium leprae (348 aa), FASTA scores: opt: 1775, E(): 2.3e-102, (76.45% identity in 340 aa overlap). Also similar to others and other substrate-binding proteins e.g. P27366|SUBI_SYNP7|SBPA sulfate-binding protein precursor from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (350 aa), FASTA scores: opt: 703, E(): 4.6e-36, (35.6% identity in 351 aa overlap); Q9I6K7|SBP|PA0283 sulfate-binding protein precursor from Pseudomonas aeruginosa (332 aa), FASTA scores: opt: 591, E(): 3.7e-29, (36.9% identity in 317 aa overlap); CAC49112|SMB21133 putative sulfate uptake ABC transporter periplasmic solute-binding protein precursor from Rhizobium meliloti (Sinorhizobium meliloti) (341 aa), FASTA scores: opt: 569, E(): 8.8e-28, (36.15% identity in 321 aa overlap); etc. Belongs to the prokaryotic sulfate binding protein family. P71744 No cell wall and cell processes P71744 GO:0015419,GO:0008272,GO:0030288 P71744 NC_000962.3 Mycobrowser_v5 CDS 2701287 2703248 . - 0 Rv2404c lepA Involved in translation. Probable GTP-binding protein LepA (GTP-binding elongation factor) Rv2404c, (MT2476, MTCY253.16), len: 653 aa. Probable lepA, GTP-binding protein (a protein of unknown function, but apparently with membrane-related functions and very similar to protein synthesis elongation factors; see citations below). Equivalent to P53530|LEPA_MYCLE|ML0611|B1937_F3_81 GTP-binding protein from Mycobacterium leprae (646 aa), FASTA scores: opt: 3610, E(): 1.2e-205, (88.0% identity in 649 aa overlap). Also highly similar to many GTP-binding proteins LEPA e.g. Q9RDC9|LEPA_STRCO|SCC77.29c from Streptomyces coelicolor (622 aa), FASTA scores: opt: 3046, E(): 2.3e-172, (74.3% identity in 626 aa overlap); P37949|LEPA_BACSU from B. subtilis (612 aa), FASTA scores: opt: 2430, E(): 5.3e-136, (58.7% identity in 610 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00301 GTP-binding elongation factors signature. Belongs to the GTP-binding elongation factor family, LEPA subfamily. P9WK97 No intermediary metabolism and respiration P65269 P9WK97 GO:0003924,GO:0005525 P9WK97 NC_000962.3 Mycobrowser_v5 CDS 2721866 2723308 . - 0 Rv2425c Rv2425c Function unknown Conserved hypothetical protein Rv2425c, (MTCY428.22), len: 480 aa. Hypothetical protein; C-terminal half shares similarity to other unknown conserved proteins e.g. Q53065 hypothetical 24.3 KDA protein from Rhodococcus erythropolis (219 aa), FASTA scores: opt: 398, E(): 9.9e-17, (34.15% identity in 202 aa overlap); C-terminus of O27843|MTH1815 conserved protein from Methanothermobacter thermautotrophicus (346 aa), FASTA scores: opt: 341, E(): 3.7e-13, (31.35% identity in 233 aa overlap); etc. P71923 No conserved hypotheticals P71923 P71923 P71923 NC_000962.3 Mycobrowser_v5 CDS 2706494 2707333 . - 0 Rv2409c Rv2409c Function unknown Conserved protein Rv2409c, (MTCY253.11), len: 279 aa. Conserved protein, equivalent to Q49757|YP69_MYCLE|G466976|B1937_F2_39 hypothetical protein from Mycobacterium leprae (279 aa), FASTA scores: opt: 1564, E(): 4.6e-95, (82.1% identity in 279 aa overlap). Also similar to others e.g. Q9RSX6|DR1993 from Deinococcus radiodurans (274 aa), FASTA scores: opt: 494, E(): 4e-25, (35.1% identity in 282 aa overlap); BAB49898|Mll2875 from Rhizobium loti (Mesorhizobium loti) (294 aa), FASTA scores: opt: 382, E(): 8.9e-18, (29.75% identity in 269 aa overlap); Q9I305|PA1732 from Pseudomonas aeruginosa (266 aa), FASTA scores: opt: 326, E(): 3.7e-14, (31.25% identity in 275 aa overlap); etc. Also similar to Rv2569c|MTCY227.32 from Mycobacterium tuberculosis. P71734 No conserved hypotheticals P71734 P71734 P71734 NC_000962.3 Mycobrowser_v5 CDS 2708310 2709965 . - 0 Rv2411c Rv2411c Function unknown Conserved hypothetical protein Rv2411c, (MTCY253.09c), len: 551 aa. Hypothetical protein, highly similar to Q49755|YO11_MYCLE|ML0605|MLCL536.05c|U1937B|B1937_F1_4 hypothetical 61.8 KDA protein from Mycobacterium leprae (561 aa), FASTA scores, opt: 3163, E(): 4.1e-178, (87.35% identity in 554 aa overlap). Also highly similar, except in N-terminus, to others e.g. Q55587|Y335_SYNY3|SLL0335 hypothetical protein from Synechocystis sp. strain PCC 6803 (481 aa), FASTA scores: opt: 1620, E(): 1.2e-87, (52.8% identity in 468 aa overlap); Q9I307|PA1730 hypothetical protein from Pseudomonas aeruginosa (470 aa), FASTA scores: opt: 1574, E(): 5.8e-85, (52.7% identity in 467 aa overlap); Q9RST1|DR2042 conserved hypothetical protein from Deinococcus radiodurans (655 aa), FASTA scores: opt: 1561, E(): 4.4e-84, (53.3% identity in 467 aa overlap); etc. P9WLA9 No conserved hypotheticals P65001 P9WLA9 P9WLA9 NC_000962.3 Mycobrowser_v5 CDS 2712891 2713784 . - 0 Rv2415c Rv2415c Function unknown Conserved hypothetical protein Rv2415c, (MTCY253.05), len: 297 aa. Hypothetical protein, with some similarity in C-terminal part to comE operon proteins 1 e.g. Q9EU10|come|COME4|COME1|COME2|COME3 come protein (a competence protein with DNA-binding activity) from Neisseria gonorrhoeae (99 aa), FASTA scores: opt: 190, E(): 0.0032, (49.2% identity in 61 aa overlap); Q9JYB8|NMB1657 from Neisseria meningitidis (205 aa) FASTA scores: opt: 191, E(): 0.0052, (49.2% identity in 61 aa overlap); CME1_BACSU|P39694 come operon protein 1 from Bacillus subtilis (205 aa), FASTA scores, opt: 181, E(): 0.017 (29.8% identity in 218 aa overlap); etc. P71728 No conserved hypotheticals P71728 P71728 GO:0006281,GO:0003677 P71728 NC_000962.3 Mycobrowser_v5 CDS 2724230 2725477 . - 0 Rv2427c proA Involved in proline biosynthesis pathway (at the second step). Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. [catalytic activity: L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-gamma-glutamyl 5-phosphate + NADPH]. Probable gamma-glutamyl phosphate reductase protein ProA (GPR) (glutamate-5-semialdehyde dehydrogenase) (glutamyl-gamma-semialdehyde dehydrogenase) Rv2427c, (MTCY428.20), len: 415 aa. Probable proA, gamma-glutamyl phosphate reductase protein, equivalent to Q9CBZ7|ML1458|PROA [gamma]-glutamyl phosphate reductase from Mycobacterium leprae (409 aa), FASTA scores: opt: 2120, E(): 7.4e-118, (81.9% identity in 409 aa overlap). Also highly similar or similar to other gamma-glutamyl phosphate reductases proteins (GPR) e.g. Q9RDK1|PROA from Streptomyces coelicolor (428 aa), FASTA scores: opt: 1073, E(): 4.6e-56, (60.4% identity in 429 aa overlap); P45638|PROA_CORGL from Corynebacterium glutamicum (432 aa), FASTA scores: opt: 993, E(): 2.4e-51, (58.5% identity in 417 aa overlap); P96489|PROA_STRTR gamma-glutamyl phosphate reductase from Streptococcus thermophilus (416 aa), FASTA scores: opt: 863, E(): 1.1e-43, (49.15% identity in 413 aa overlap); etc. Belongs to the gamma-glutamyl phosphate reductase family. P9WHV1 No intermediary metabolism and respiration P65788 P9WHV1 GO:0005737,GO:0004350,GO:0055114,GO:0006561,GO:0050661 1.2.1.41 P9WHV1 NC_000962.3 Mycobrowser_v5 CDS 2726806 2727339 . + 0 Rv2429 ahpD Involved in oxidative stress response. LPDC|Rv0462, DLAT|Rv2215, AHPD|Rv2429, and AHPC|Rv2428 constitute an NADH-dependent peroxidase and peroxynitrite reductase that provides protection against oxidative stress. Alkyl hydroperoxide reductase D protein AhpD (alkyl hydroperoxidase D) Rv2429, (MTCY428.17c), len: 177 aa. AhpD, alkyl hydroperoxide reductase, similar to other alkyl hydroperoxide reductases D proteins e.g. Q9RN73|AHPD from Streptomyces coelicolor (178 aa), FASTA scores: opt: 611, E(): 1.4e-33, (57.4% identity in 169 aa overlap); Q50441|AHPD_MYCSM AHPD protein (fragment) from Mycobacterium smegmatis (52 aa), FASTA score: opt:196. P9WQB5 No virulence, detoxification, adaptation 1GU9,1KNC,1LW1,1ME5 P0A5N4 P9WQB5 GO:0055114,GO:0004601,GO:0051920,GO:0008785 1.11.1.15 P9WQB5 NC_000962.3 Mycobrowser_v5 CDS 2728844 2729134 . - 0 Rv2433c Rv2433c Unknown Hypothetical protein Rv2433c, (MTCY428.13), len: 96 aa. Hypothetical unknown protein. P71916 No conserved hypotheticals P71916 NC_000962.3 Mycobrowser_v5 CDS 2729115 2730560 . - 0 Rv2434c Rv2434c Unknown Probable conserved transmembrane protein Rv2434c, (MTCY428.12), len: 481 aa. Probable conserved transmembrane protein, with some similarity to BAB48444|MLR0973 probable integral membrane protein from Rhizobium loti (410 aa), FASTA scores: opt: 298, E(): 4.1e-11, (27.25% identity in 389 aa overlap); and also similarity with other hypothetical proteins and/or putative integral membrane proteins. P71915 No cell wall and cell processes P71915 P71915 GO:0055085,GO:0016021 P71915 NC_000962.3 Mycobrowser_v5 CDS 2733230 2734144 . + 0 Rv2436 rbsK Involved in ribose metabolism (in the first step) [catalytic activity: ATP + D-ribose = ADP + D-ribose 5-phosphate]. Ribokinase RbsK Rv2436, (MTCY428.10c), len: 304 aa. Probable rbsK, ribokinase, similar to others e.g. Q9RZ99|DRA0055 from Deinococcus radiodurans (300 aa) FASTA scores: opt: 485, E(): 9.1e-21, (44.55% identity in 301 aa overlap); P36945|P96733|RBSK_BACSU from Bacillus subtilis (293 aa), FASTA scores: opt: 398, E(): 8.5e-16, (36.35% identity in 297 aa overlap); P05054|RBSK_ECOLI|B3752|Z5253|ECS4694 from Escherichia coli strain K12 (309 aa), FASTA scores: opt: 387, E(): 3.8e-15, (34.7% identity in 314 aa overlap); etc. Contains PS00583 pfkB family of carbohydrate kinases signature 1. Belongs to the PFKB family of carbohydrate kinases. P71913 No intermediary metabolism and respiration 3GO6,3GO7 P71913 P71913 GO:0004747,GO:0006014 2.7.1.15 P71913 NC_000962.3 Mycobrowser_v5 CDS 2734792 2736831 . - 0 Rv2438c nadE Involved in biosynthesis of NAD. Can use both glutamine or ammonia as a nitrogen source [catalytic activity: ATP + deamido-NAD(+) + L-glutamine + H(2)O = AMP + diphosphate + NAD(+) + L-glutamate]. Glutamine-dependent NAD(+) synthetase NadE (NAD(+) synthase [glutamine-hydrolysing]) Rv2438c, (MT2513, MTCY428.08), len: 679 aa. NadE, glutamine-dependent NAD(+) synthetase (see citation below), equivalent to Q9CBZ6|NADE_MYCLE|ML1463 Glutamine-dependent NAD(+) synthetase from Mycobacterium leprae (680 aa), FASTA scores: opt: 3877, E(): 0. Also similar to others e.g. O83759|NADE_TREPA|TP0780 from Treponema pallidum (679 aa), FASTA scores: opt: 543, E(): 1.1e-25; O74940|NADE_SCHPO|SPCC553.02 from Schizosaccharomyces pombe (Fission yeast) (700 aa), FASTA scores: opt: 354, E(): 4.7e-14 ; P38795|NADE_YEAST|YHR074W from Saccharomyces cerevisiae (Baker's yeast) (714 aa), FASTA scores: opt: 339, E(): 4e-13; etc. Contains PS00591 Glycosyl hydrolases family 10 active site. Belongs to the NAD synthetase family in the C-terminal section. N-terminus shorter since first submission. P9WJJ3 No intermediary metabolism and respiration 3DLA P0A5L6 P9WJJ3 GO:0009435,GO:0003952,GO:0016810,GO:0005524 6.3.5.1 P9WJJ3 NC_000962.3 Mycobrowser_v5 CDS 2737117 2738247 . - 0 Rv2439c proB Involved in proline biosynthesis pathway (at the first step). Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline [catalytic activity: ATP + L-glutamate = ADP + L-glutamate 5- phosphate]. Probable glutamate 5-kinase protein ProB (gamma-glutamyl kinase) (GK) Rv2439c, (MTCY428.07), len: 376 aa. Probable proB, glutamate 5-kinase protein (GK), equivalent to Q9CBZ5|prob|ML1464 from Mycobacterium leprae (367 aa) FASTA scores: opt: 1937, E(): 1.1e-102, (84.4% identity in 366 aa overlap). Also highly similar to other glutamate 5-kinase proteins e.g. P46546|PROB_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (369 aa), FASTA scores: opt: 1241, E(): 3e-63, (54.35% identity in 368 aa overlap); Q9ZG98|PROB_MEIRU glutamate 5-kinase from Meiothermus ruber (390 aa), FASTA scores: opt: 825, E(): 1.2e-39, (45.05% identity in 353 aa overlap); Q9RDJ9|prob|SCC123.25c from Streptomyces coelicolor (374 aa), FASTA scores: opt: 1193, E(): 1.6e-60, (55.85% identity in 367 aa overlap); etc. Contains PS00902 Glutamate 5-kinase signature. Belongs to the glutamate 5-kinase family. P9WHU9 No intermediary metabolism and respiration P71910 P9WHU9 GO:0003723,GO:0005737,GO:0004349,GO:0006561,GO:0005524 2.7.2.11 P9WHU9 NC_000962.3 Mycobrowser_v5 CDS 2752262 2752660 . + 0 Rv2451 Rv2451 Unknown Hypothetical proline and serine rich protein Rv2451, (MTV008.07), len: 132 aa. Hypothetical unknown pro-, ser-rich protein. O53178 No conserved hypotheticals O53178 O53178 NC_000962.3 Mycobrowser_v5 CDS 2738247 2739686 . - 0 Rv2440c obg Essential GTP-binding protein. Probable GTP1/Obg-family GTP-binding protein Obg Rv2440c, (MTCY428.06), len: 479 aa. Probable obg, nucleotide-binding protein, equivalent to Q9CBZ4|ML1465 GTP1/OBG-family GTP-binding protein from Mycobacterium leprae (478 aa), FASTA scores: opt: 1328, E(): 8.4e-70, (58.9% identity in 479 aa overlap). Also highly similar to others e.g. P95722|OBG GTP-binding protein from Streptomyces coelicolor (478 aa), FASTA scores: opt: 1311, E(): 8.2e-69, (60.7% identity in 476 aa overlap); P20964|OBG_BACSU SPO0B-associated GTP-binding protein from Bacillus subtilis (428 aa), FASTA scores: opt: 1006, E(): 3.9e-51, (42.9% identity in 436 aa overlap); Q9KDK0|OBG|BH1213 GTP-binding protein involved in initiation of sporulation from Bacillus halodurans (427 aa), FASTA scores: opt: 978, E(): 1.7e-49, (41.95% identity in 436 aa overlap); etc. Highly similar (identical but shorter 5 aa) to AAK46813|MT2516 GTP-binding protein from Mycobacterium tuberculosis strain CDC1551 (484 aa), FASTA scores: opt: 3205, E(): 7.9e-179, (100% identity in 479 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the GTP1/OBG family. P9WMT1 No intermediary metabolism and respiration P71909 P9WMT1 GO:0003924,GO:0005737,GO:0005525 P9WMT1 NC_000962.3 Mycobrowser_v5 CDS 2740709 2742184 . + 0 Rv2443 dctA Involved in the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the inner membrane. Responsible for the translocation of the substrate across the membrane. Probable C4-dicarboxylate-transport transmembrane protein DctA Rv2443, (MTCY428.03c), len: 491 aa. Probable dctA, C4-dicarboxylate-transport transmembrane protein, similar to other C4-dicarboxylate transport proteins e.g. AAK46817|MT2519 from Mycobacterium tuberculosis strain CDC1551 (491 aa); Q9L1K8|SC6A11.12 putative sodium:dicarboxylate symporter from Streptomyces coelicolor (466 aa), FASTA scores: opt: 1797, E(): 2.9e-98, (61.3% identity in 452 aa overlap); Q9RRG7|DR2525 from Deinococcus radiodurans (463 aa); P50334|DCTA_SALTY from Salmonella typhimurium (428 aa) FASTA scores: opt: 1241, E(): 1.3e-65, (47.2% identity in 415 aa overlap); etc. Belongs to the sodium dicarboxylate symporter family (SDF) (DAACS family). P71906 No cell wall and cell processes P71906 P71906 GO:0016021,GO:0017153,GO:0006835 P71906 NC_000962.3 Mycobrowser_v5 CDS 2746135 2747598 . - 0 Rv2447c folC Conversion of folates to polyglutamate derivatives. Bacteria require folate for the biosynthesis of glycine, methionine, formyl-met-tRNA, thymidylates, purines, and pantothenate [catalytic activity: ATP + {tetrahydrofolyl-[GLU]}(N) + L-glutamate = ADP + phosphate + {tetrahydrofolyl-[GLU]}(N+1)]. Probable folylpolyglutamate synthase protein FolC (folylpoly-gamma-glutamate synthetase) (FPGS) Rv2447c, (MTV008.03c), len: 487 aa. Probable folC, folylpolyglutamate synthase, equivalent to Q9CBY8|FOLC|ML1471 from Mycobacterium leprae (485 aa), FASTA scores: opt: 2425, E(): 2.2e-134, (78.7% identity in 483 aa overlap). Also highly similar to others e.g. Q9L1G4|FPGS|O08416|Y13070 from Streptomyces coelicolor (444 aa), FASTA scores: opt: 774, E(): 6.3e-38, (53.9% identity in 462 aa overlap); P15925|FOLC_LACCA|FGS from Lactobacillus casei (428 aa), FASTA scores: opt: 631, E(): 1.4e-29, (34.55% identity in 437 aa overlap); Q05865|FOLC_BACSU from Bacillus subtilis (430 aa), FASTA scores: opt: 421, E(): 2.6e-17, (32.9% identity in 383 aa overlap); etc. Contains PS01012 Folylpolyglutamate synthase signature 2. Belongs to the folylpolyglutamate synthase family. I6Y0R5 No intermediary metabolism and respiration 2VOR,2VOS O53174 I6Y0R5 GO:0009396,GO:0004326,GO:0005524 6.3.2.17 I6Y0R5 NC_000962.3 Mycobrowser_v5 CDS 2747595 2750225 . - 0 Rv2448c valS Involved in translation mechanisms [catalyic activity: ATP + L-valine + tRNA(val) = AMP + diphosphate + L-valyl-tRNA(val)]. Probable valyl-tRNA synthase protein ValS (valyl-tRNA synthetase) (valine--tRNA ligase) (valine translase) Rv2448c, (MTV008.04c), len: 876 aa. Probable valS, valyl-tRNA synthetases, equivalent to Q9CBY7|VALS|ML1472 valyl-tRNA synthase from Mycobacterium leprae (886 aa), FASTA scores: opt: 5181,E(): 0, (85.4% identity in 876 aa overlap). Also highly similar to others e.g. O06851|SYV_STRCO from Streptomyces coelicolor (874 aa), FASTA scores: opt: 2470, E(): 1.6e-143, (60.45% identity in 880 aa overlap); Q9X2D7|SYV_THEMA|VALS|TM1817 from Thermotoga maritima (865 aa), FASTA scores: opt: 2418, E(): 2.4e-140, (44.2% identity in 891 aa overlap); Q05873|SYV_BACSU|VALS from Bacillus subtilis (880 aa), FASTA scores: opt: 2063, E(): 1.4e-118, (46.08% identity in 894 aa overlap); etc. Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. Contains probable coiled-coil from aa 810 to 846. Belongs to class-I aminoacyl-tRNA synthetase family. P9WFS9 No information pathways P67599 P9WFS9 GO:0005737,GO:0004832,GO:0006438,GO:0005524 6.1.1.9 P9WFS9 NC_000962.3 Mycobrowser_v5 CDS 2751662 2752180 . - 0 Rv2450c rpfE Thought to promote the resuscitation and growth of dormant, nongrowing cells. Could also stimulate the growth of several other high G+C gram+ organisms, e.g. Mycobacterium avium, Mycobacterium bovis (BCG), Mycobacterium kansasii, Mycobacterium smegmatis. Probable resuscitation-promoting factor RpfE Rv2450c, (MTV008.06c), len: 172 aa. Probable rpfE, resuscitation-promoting factor (see Mukamolova et al., 1998), similar to O86308|Z96935|MLRPF_1 RPF protein precursor from Micrococcus luteus (220 aa), FASTA scores: opt: 291, E(): 3e-7, (48.75% identity in 80 aa overlap). C-terminus is similar to other Mycobacterial rpf proteins e.g. O05594|Rv1009|MTCI237.26|RPFB probable resuscitation-promoting factor from Mycobacterium tuberculosis (362 aa), FASTA scores: opt: 344, E(): 1.4e-09, (42.85% identity in 147 aa overlap); etc. C-terminal region similar to N-terminal region of Q9F2Q2|SCE41.06c putative secreted protein from Streptomyces coelicolor (244 aa), FASTA scores: opt: 355, E(): 3.1e-10, (56.65% identity in 90 aa overlap). Also similar to Q9F2Q1|SCE41.07c putative secreted protein from Streptomyces coelicolor (near Q9F2Q2|SCE41.06c) (341 aa) FASTA scores: opt: 317, E(): 2.5e-08, (51.7% identity in 87 aa overlap). With Mycobacterium leprae, high similarity between the two corresponding C-terminal regions of two hypothetical proteins, Q9CD53|ML0240 (375 aa), FASTA scores: opt: 339, E(): 2.5e-09, (59.15% identity in 93 aa overlap) and O33049|MLCB57.05c|ML2151 (174 aa), FASTA scores: opt: 329, E(): 4e-09, (58.14% identity in 86 aa overlap). Contains a possible secretory signal sequence in N-terminus. Possible autocrine and/or paracrine bacterial growth factor or cytokine (see citations below). Interacts with RipA (see Hett et al., 2007). Predicted possible vaccine candidate (See Zvi et al., 2008). O53177 No cell wall and cell processes O53177 O53177 GO:0005576 O53177 NC_000962.3 Mycobrowser_v5 CDS 2758208 2759488 . - 0 Rv2457c clpX ATP-dependent specificity component of the CLP protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of CLPP). Probable ATP-dependent CLP protease ATP-binding subunit ClpX Rv2457c, (MTV008.13c), len: 426 aa. Probable clpX, ATP-dependent clp protease ATP-binding subunit clpX, equivalent to Q9CBY6|CLPX|ML1477 ATP-dependent CLP protease ATP-binding protein from Mycobacterium leprae (426 aa), FASTA scores: opt: 2652, E(): 1.4e-142, (96.0% identity in 426 aa overlap). Also highly similar to others e.g. Q9F316|CLPX from Streptomyces coelicolor (428 aa) FASTA scores: opt: 2178, E(): 8.2e-116, (77.8% identity in 428 aa overlap); P50866|CLPX_BACSU from Bacillus subtilis (420 aa), FASTA scores: opt: 1788, E(): 8.5e-94, (63.6% identity in 426 aa overlap); P33138|CLPX_ECOLI from Escherichia coli (423 aa), FASTA scores: opt: 1694, E(): 1.7e-88, (62.4% identity in 415 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the CLPX chaperone family. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P9WPB9 No intermediary metabolism and respiration P0A528 P9WPB9 GO:0016887,GO:0046983,GO:0006457,GO:0051082,GO:0008270,GO:0005524 P9WPB9 NC_000962.3 Mycobrowser_v5 CDS 2763172 2763774 . - 0 Rv2461c clpP1 CLP cleaves peptides in various proteins in a process that requires ATP hydrolysis. CLP may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. Probable ATP-dependent CLP protease proteolytic subunit 1 ClpP1 (endopeptidase CLP) Rv2461c, (MT2536, MTV008.17c), len: 200 aa. Probable clpP1, ATP-dependent clp protease proteolytic subunit 1, equivalent to Q9CBY3|CLP1_MYCLE ATP-dependent CLP protease proteolytic subunit from Mycobacterium leprae (224 aa), FASTA scores: opt: 1226, E(): 1.3e-71, (95.0% identity in 200 aa overlap). Also highly similar to others e.g. Q9F315|CLPP1 from Streptomyces coelicolor (219 aa), FASTA scores: opt: 713, E(): 9.3e-39, (61.75% identity in 183 aa overlap); P80244|CLPP_BACSU from Bacillus subtilis (197 aa), FASTA scores: opt: 658, E(): 2.8e-35, (54% identity in 187 aa overlap); Q9WZF9|CLPP_THEMA|TM0695 from Thermotoga maritima (203 aa), FASTA scores: opt: 653, E(): 6.1e-35, (55.25% identity in 172 aa overlap); etc. Also similar to downstream ORF Rv2460c|MTV008.16c|clpP2 (214 aa), FASTA score: (48.3% identity in 172 aa overlap). Belongs to peptidase family S14, also known as CLPP family. Note that previously known as clp. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P9WPC5 No intermediary metabolism and respiration 2C8T,2CBY,2CE3 P0A526 P9WPC5 GO:0005737,GO:0006508,GO:0004252,GO:0005524 3.4.21.92 P9WPC5 NC_000962.3 Mycobrowser_v5 CDS 2924817 2925383 . - 0 Rv2594c ruvC Nuclease that resolves holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous ARMS and leaves a 5'terminal phosphate and a 3'terminal hydroxyl group [catalytic activity: endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (holliday junction)]. Probable crossover junction endodeoxyribonuclease RuvC (holliday junction nuclease) (holliday junction resolvase) Rv2594c, (MTCY227.07), len: 188 aa. Probable ruvC, Holliday junction resolvase (see citations below), equivalent to P40834|RUVC_MYCLE|ML0481|B1177_C3_226 crossover junction endodeoxyribonuclease from Mycobacterium leprae (188 aa), FASTA scores: opt: 984, E(): 2.3e-55, (81.0% identity in 184 aa overlap). Also highly similar to others e.g. Q9AE11|RUVC from Corynebacterium glutamicum (Brevibacterium flavum) (221 aa), FASTA scores: opt: 713, E(): 3.6e-38, (56.9% identity in 188 aa overlap); Q9L289|RUVC_STRCO|SCL2.10c from Streptomyces coelicolor (188 aa), FASTA scores: opt: 704, E(): 1.2e-37, (60.65% identity in 178 aa overlap); P24239|RUVC_ECOLI|B1863 from Escherichia coli strain K12 (172 aa), FASTA scores: opt: 322, E(): 1.6e-13, (38.65% identity in 163 aa overlap); etc. Belongs to the RUVC family. Cofactor: magnesium. P9WGV9 No information pathways P66760 P9WGV9 GO:0006281,GO:0008821,GO:0000287,GO:0003676,GO:0006310 3.1.22.4 P9WGV9 NC_000962.3 Mycobrowser_v5 CDS 2765655 2766839 . + 0 Rv2463 lipP Function unknown; lipolytic enzyme involved in cellular metabolism. Probable esterase/lipase LipP Rv2463, (MTV008.19), len: 394 aa. Probable lipP, esterase, lipase similar to others eg O87861|ESTA esterase a from Streptomyces chrysomallus (389 aa), FASTA scores: opt: 964, E(): 1.9e-53, (44.35% identity in 399 aa overlap); Q9I4S7|PA1047 probable esterase from Pseudomonas aeruginosa (392 aa), FASTA scores: opt: 863, E(): 4.6e-47, (40.05% identity in 377 aa overlap); Q53403|ESTC esterase III from Pseudomonas fluorescens (382 aa), FASTA scores: opt: 753, E(): 3.9e-40, (36.3% identity in 380 aa overlap); etc. O53190 No intermediary metabolism and respiration O53190 O53190 GO:0016787 3.1.-.- O53190 NC_000962.3 Mycobrowser_v5 CDS 2767671 2768159 . - 0 Rv2465c rpiB Interconverts ribose-5-phosphate and ribulose-5-phosphate Ribose-5-phosphate isomerase Rv2465c, (MTV008.21c), len: 162 aa. RpiB, Ribose-5-phosphate isomerase, proven biochemically (see Roos et al., 2004) equivalent to AAK46840|MT2540 putative carbohydrate-phosphate isomerase from Mycobacterium tuberculosis strain CDC1551 (159 aa). Equivalent to Q9CBY1|ML1484 possible phosphopentose isomerase from Mycobacterium leprae (162 aa), FASTA scores: opt: 992, E(): 7.1e-59, (89.5% identity in 162 aa overlap). Also highly similar or similar to several diverse isomerases e.g. Q9L206|SC8E4.02c putative isomerase from Streptomyces coelicolor (159 aa), FASTA scores: opt: 661, E(): 6.1e-37, (61.45% identity in 153 aa overlap); P47636|Y396_MYCGE|MG396 hypothetical LACA/RPIB family protein from Mycoplasma genitalium (152 aa), FASTA scores: opt: 357, E(): 8.2e-17, (42% identity in 150 aa overlap); P53527|Y396_MYCPN|MPN595|MP247 hypothetical LACA/RPIB family protein from Mycoplasma pneumoniae (152 aa), FASTA scores: opt: 340, E(): 1.1e-15, (38.6% identity in 145 aa overlap); P26592|LACB_STAAU galactose-6-phosphate isomerase from Staphylococcus aureus (171 aa), FASTA scores: opt: 296, E(): 1e-12, (35.4% identity in 158 aa overlap) and P37351|RPIB_ECOLI ribose 5-phosphate isomerase b from Escherichia coli (149 aa), FASTA scores: opt: 262, E(): 1.6e-10, (32.2% identity in 146 aa overlap); etc. Could belong to the LACA/RPIB family. P9WKD7 No intermediary metabolism and respiration 1USL,2BES,2BET,2VVO,2VVP,2VVQ Q79FD7 P9WKD7 GO:0004751,GO:0005975 5.3.1.6 P9WKD7 NC_000962.3 Mycobrowser_v5 CDS 2772367 2773035 . - 0 Rv2469c Rv2469c Function unknown Conserved hypothetical protein Rv2469c, (MTV008.25c), len: 222 aa. Conserved hypothetical protein, highly similar to other hypothetical proteins e.g. Q9X7B4|MLCB1610.15|ML1254 from Mycobacterium leprae (215 aa), FASTA scores: opt: 1183, E(): 3.3e-70, (77.9% identity in 222 aa overlap); Q9L1Y0|SC8E4A.25c from Streptomyces coelicolor (178 aa), FASTA scores: opt: 589, E(): 1.7e-31, (53.4% identity in 161 aa overlap) (N-terminal region is shorter 50 aa approximately); Q9RRS6|DR2409 conserved hypothetical protein from Deinococcus radiodurans (186 aa), FASTA scores: opt: 440, E(): 9.6e-22, (42.25% identity in 168 aa overlap) (N-terminal region is shorter 30 aa approximately); etc. O53196 No conserved hypotheticals O53196 O53196 GO:0003676,GO:0004519 O53196 NC_000962.3 Mycobrowser_v5 CDS 2773564 2775204 . + 0 Rv2471 aglA Involved in sugar metabolism (hydrolysis of terminal, non-reducing 1,4-linked D-glucose residues with release of D-glucose). Probable alpha-glucosidase AglA (maltase) (glucoinvertase) (glucosidosucrase) (maltase-glucoamylase) (lysosomal alpha-glucosidase) (acid maltase) Rv2471, (MTV008.27), len: 546 aa. Probable aglA, maltase (alpha-glucosidase), highly similar or similar to several e.g. Q60027|AGLA from Thermomonospora curvata (544 aa), FASTA scores: opt: 2071, E(): 4e-116, (57.7% identity in 525 aa overlap); Q9KZE3|AGLAE from Streptomyces coelicolor (534 aa), FASTA scores: opt: 1475, E(): 1.5e-80, (50.1% identity in 537 aa overlap); O86874|AGLA from Streptomyces lividans (534 aa), FASTA scores: opt: 1473, E(): 2e-80, (50.1% identity in 537 aa overlap); etc. Seems to belong to family 13 of glycosyl hydrolases, also known as the alpha-amylase family. O53198 No intermediary metabolism and respiration O53198 O53198 GO:0005975,GO:0043169,GO:0004558 3.2.1.20 O53198 NC_000962.3 Mycobrowser_v5 CDS 2823256 2824593 . - 0 Rv2508c Rv2508c Unknown Probable conserved integral membrane leucine and alanine rich protein Rv2508c, (MTCY07A7.14c), len: 445 aa. Probable conserved integral membrane leu-, ala-rich protein, equivalent to Q9CCU4|ML0430 putative membrane protein from Mycobacterium leprae (454 aa) (alias O07710|MLCL383.37 longer 10 aa), FASTA scores: opt: 2205, E(): 2.5e-124, (75.75% identity in 441 aa overlap). Also similar to hypothetical or membrane proteins e.g. BAB50841|MLL4103 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (458 aa), FASTA scores: opt: 396, E(): 2.4e-16, (27.75% identity in 447 aa overlap); Q9RKX9|SC6D7.19c putative integral membrane protein from Streptomyces coelicolor (486 aa), FASTA scores: opt: 323, E(): 5.7e-12, (28.95% identity in 428 aa overlap); P42306|YXIO_BACSU probable integral membrane protein from Bacillus subtilis (428 aa), FASTA scores: opt: 220, E(): 7.2e-06, (20.35% identity in 413 aa overlap); etc. Also similar to proteins from Mycobacterium tuberculosis e.g. Q10564|Y876_MYCTU|Rv0876c|MT0899|MTCY31.04c (548 aa), FASTA scores: opt: 184, E(): 0.0012, (24.7% identity in 466 aa overlap). O06171 No cell wall and cell processes O06171 NC_000962.3 Mycobrowser_v5 CDS 2777388 2782262 . - 0 Rv2476c gdh Catabolic glutdh involved in the utilization of glutamate and other amino acids of the glutamate family. Generates 2-oxoglutarate from L-glutamate [catalytic activity: L-glutamate + H(2)O + NAD(+) = 2-oxoglutarate + NH(3) + NADH]. Probable NAD-dependent glutamate dehydrogenase Gdh (NAD-Gdh) (NAD-dependent glutamic dehydrogenase) Rv2476c, (MTV008.32c), len: 1624 aa. Probable gdh, glutamate dehydrogenase. Highly similar to Q9X7B2|MLCB1610.10|ML1249 hypothetical 177.9 KDA protein from Mycobacterium leprae (1622 aa), FASTA scores: opt: 8630,E(): 0, (81.45% identity in 1634 aa overlap). But highly similar to Q9F0J1|GDH NAD-glutamate dehydrogenase from Streptomyces clavuligerus (1651 aa), FASTA scores: opt: 3833, E(): 0, (45.8% identity in 1600 aa overlap); (see Minambres et al., 2000). Also similar with others e.g. AAG53963|PA3068|GDHB hypothetical (NAD(+)-dependent glutamate dehydrogenase from Pseudomonas aeruginosa (1620 aa), FASTA scores: opt: 2214, E(): 1e-124, (40.1% identity in 1561 aa overlap) (see Lu & Abdelal 2001); and Q9Y8G5|GDHB NAD-specific glutamate dehydrogenase from Agaricus bisporus (1029 aa), FASTA scores: opt: 194, E(): 0.00099, (22.7% identity in 647 aa overlap) (see Kersten et al., 1999); etc. Contains possible Helix-turn-helix motif at aa 1568 to 1589 (score 1098, +2.93 SD). O53203 No intermediary metabolism and respiration O53203 O53203 GO:0004352,GO:0055114 1.4.1.2 NC_000962.3 Mycobrowser_v5 CDS 2786914 2789283 . - 0 Rv2482c plsB2 Involved in phospholipid biosynthesis (at the first step). May also function in the regulation of membrane biogenesis [catalytic activity: acyl-CoA + SN-glycerol 3-phosphate = CoA + 1-acyl-SN-glycerol 3-phosphate]. Probable glycerol-3-phosphate acyltransferase PlsB2 (GPAT) Rv2482c, (MT2555, MTV008.38c), len: 789 aa. Probable plsB2, glycerol-3-phosphate acyltransferase, highly similar to Q9X7B0|PLSB_MYCLE probable glycerol-3-phosphate acyltransferase from Mycobacterium leprae (775 aa), FASTA scores: opt: 4210, E(): 0, (80.7% identity in 783 aa overlap). Also similar to others e.g. P00482|PLSB_ECOLI from Escherichia coli (806 aa), FASTA scores: opt: 521, E(): 3e-24, (24.35 identity in 612 aa overlap); Q9CLN7|PLSB_PASMU from Pasteurella multocida (809 aa), FASTA scores: opt: 529, E(): 9.7e-25, (27.05% identity in 540 aa overlap); Q9KVP8|PLSB_VIBCH from Vibrio cholerae (811 aa), FASTA scores: opt: 510, E(): 1.4e-23, (26.0% identity in 639 aa overlap); etc. Also highly similar to Q10775|PLSB1|Rv1551|MTCY48.14c from M. tuberculosis (621 aa), FASTA scores: opt: 1013, E(): 1.5e-54, (34.65% identity in 586 aa overlap). Belongs to the GPAT/DAPAT family. P9WI61 No lipid metabolism O53207 P9WI61 GO:0008654,GO:0005886,GO:0004366 2.3.1.15 P9WI61 NC_000962.3 Mycobrowser_v5 CDS 2792723 2793988 . - 0 Rv2485c lipQ Function unknown; lipolytic enzyme involved in cellular metabolism. Probable carboxylesterase LipQ Rv2485c, (MTV008.41c), len: 421 aa. Probable lipQ, carboxylesterase protein (lipase). Similar (greater at the C-terminal end) to AAK46626|MT2342 putative carboxylesterase from Mycobacterium tuberculosis strain CDC1551 (431 aa), FASTA scores: opt: 1134, E(): 4.3e-60, (46.25% identity in 428 aa overlap); and Q50681|Rv2284|MTCY339.26c hypothetical protein from M. tuberculosis strain H37Rv (431 aa), FASTA scores: opt: 1134, E(): 4.3e-60, (46.25% identity in 428 aa overlap). Also similar in part to other putative lipases/esterases e.g. AAK44451|MT0230 from Mycobacterium tuberculosis strain CDC1551 (403 aa), FASTA scores: opt: 763, E(): 4.6e-38, (37.95% identity in 390 aa overlap); Q9RY19|DR0133 from Deinococcus radiodurans (296 aa), FASTA scores: opt: 392, E(): 4e-16, (33.7% identity in 276 aa overlap); Q9Z545|SC9B2.14 from Streptomyces coelicolor (502 aa) FASTA scores: opt: 279, E(): 3.2e-09, (31.15% identity in 292 aa overlap); etc. I6Y9F7 No intermediary metabolism and respiration O53210 I6Y9F7 GO:0016787 3.1.-.- I6Y9F7 NC_000962.3 Mycobrowser_v5 CDS 2801254 2806236 . - 0 Rv2490c PE_PGRS43 Function unknown PE-PGRS family protein PE_PGRS43 Rv2490c, (MTV008.46c), len: 1660 aa. PE_PGRS43, Member of the Mycobacterium tuberculosis PE family, PGRS-subfamily of Gly-rich proteins (see Brennan and Delogu, 2002), similar to many e.g. AAK47971|MT3612.1 PE_PGRS family protein from Mycobacterium tuberculosis strain CDC1551 (1715 aa), FASTA scores: opt: 5161, E(): 1.5e-187, (51.7% identity in 1752 aa overlap); etc. Q79FD4 No PE/PPE Q79FD4 Q79FD4 Q79FD4 NC_000962.3 Mycobrowser_v5 CDS 2808758 2809939 . - 0 Rv2495c bkdC Involved in energy metabolism. The branched-chain alpha-keto acid dehydrogenase complex catalyzes the overall conversion of branched chain alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Probable branched-chain keto acid dehydrogenase E2 component BkdC Rv2495c, (MTCY07A7.01c-MTV008.51c), len: 393 aa. Probable bkdC, branched-chain keto acid dehydrogenase, E2 component, similar to others e.g. Q9XA49|SCGD3.30c from Streptomyces coelicolor (491 aa) FASTA scores: opt: 615, E(): 1.2e-28, (36.45% identity in 491 aa overlap; several gaps); P19262|ODO2_YEAST|KGD2|YDR148C|YD8358.05c from Saccharomyces cerevisiae (Baker's yeast) (463 aa) FASTA scores: opt: 533, E(): 7.1e-24, (28.55% identity in 396 aa overlap); Q9HN75|DSA|VNG2219G from Halobacterium sp. strain NRC-1 (478 aa), FASTA scores: opt: 521, E(): E(): 3.7e-23, (30.25% identity in 486 aa overlap; in part); etc. Belongs to the 2-oxoacid dehydrogenase family. Alternative nucleotide at position 2809621 (T->C; T107A) has been observed. LpdC|Rv0462 co-immunoprecipitates with DlaT|Rv2215 (in lpdC|Rv0462 mutant) and with BkdC|Rv2495c (in dlaT|Rv2215 mutant) (See Venugopal et al., 2011). Previously known as pdhC. O06159 No intermediary metabolism and respiration 3L60 O06159 O06159 GO:0031405,GO:0008152,GO:0005515,GO:0004742 2.3.1.12 O06159 NC_000962.3 Mycobrowser_v5 CDS 2814916 2816880 . - 0 Rv2501c accA1 This protein carries two functions: biotin carboxyl carrier protein and biotin carboxyltransferase. Involved in the first step of long-chain fatty acid synthesis [catalytic activity: ATP + biotin-carboxyl-carrier protein + CO(2) = ADP + phosphate + carboxybiotin-carboxyl-carrier protein]. Probable acetyl-/propionyl-coenzyme A carboxylase alpha chain (alpha subunit) AccA1: biotin carboxylase + biotin carboxyl carrier protein (BCCP) Rv2501c, (MTCY07A7.07c, P46401), len: 654 aa. Probable accA1 (alternate gene name: bccA), acetyl-/propionyl-coenzyme A carboxylase (alpha subunit) [includes: biotin carboxylase ; biotin carboxyl carrier protein (BCCP)], similar to others eg Q9L076|FABG from Streptomyces coelicolor (646 aa), FASTA scores: opt: 2071, E(): 1e-113, (57.8% identity in 659 aa overlap); AAK24139|Q9A6C6|CC2168 from Caulobacter crescentus (654 aa), FASTA scores: opt: 1754, E(): 3.7e-95, (47.2% identity in 661 aa overlap); etc. Contains PS00188 Biotin-requiring enzymes attachment site, PS00866 Carbamoyl-phosphate synthase subdomain signature 1, and PS00867 Carbamoyl-phosphate synthase subdomain signature 2. P9WPQ3 No lipid metabolism P0A508 P9WPQ3 GO:0009374,GO:0004075,GO:0006633,GO:0005524 6.3.4.14 P9WPQ3 NC_000962.3 Mycobrowser_v5 CDS 2821712 2822359 . + 0 Rv2506 Rv2506 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably TetR-family) Rv2506, (MTCY07A7.12), len: 215 aa. Probable transcriptional regulator, TetR family, similar to many others e.g. Q9L078|SCC105.06c putative TetR-family regulatory protein from Streptomyces coelicolor (208 aa), FASTA scores: opt: 333, E(): 1.5e-14, (48.75% identity in 197 aa overlap); Q9X7X6|SC6A5.30c putative regulatory protein from Streptomyces coelicolor (404 aa), FASTA scores: opt: 267, E(): 4.8e-10, (30.45% identity in 207 aa overlap) (similarity only with C-terminus for this one); Q9FBI8|SCP8.33c putative TetR-family transcriptional regulator from Streptomyces coelicolor (213 aa), FASTA scores: opt: 239, E(): 1.8e-08, (29.9% identity in 184 aa overlap); etc. Also similar to transcriptional regulatory proteins from Mycobacterium tuberculosis e.g. O05858|Rv3208|MTCY07D11.18c (228 aa), FASTA scores: opt: 218, E(): 4.4e-07, (30.35% identity in 191 aa overlap); C-terminus of P95251|Rv1963c|MTV051.01c|MTCY09F9.01 (406 aa), FASTA scores: opt: 238, E(): 3.6e-08, (28.25% identity in 177 aa overlap); P96839|Rv3557c|MTCY06G11.04c (200 aa), FASTA scores: opt: 215, E(): 6.2e-07, (38.25% identity in 148 aa overlap); etc. Equivalent to AAK46885 from Mycobacterium tuberculosis strain CDC1551 (231 aa) but shorter 16 aa. Contains probable helix-turn-helix motif at aa 46-67, (Score 1660, +4.84 SD). Belongs to the TetR/AcrR family of transcriptional regulators. O06169 No regulatory proteins O06169 O06169 GO:0006350,GO:0003700,GO:0006355 O06169 NC_000962.3 Mycobrowser_v5 CDS 2925734 2926138 . + 0 Rv2596 vapC40 Unknown Possible toxin VapC40. Contains PIN domain. Rv2596, (MTCY227.05c), len: 134 aa. Possible vapC40, toxin, part of toxin-antitoxin (TA) operon with Rv2595, contains PIN domain, see Arcus et al. 2005. Similar to others in Mycobacterium tuberculosis e.g. O07780|Rv0598c|MTCY19H5.24 hypothetical 14.8 KDA protein from (137 aa), FASTA scores: opt: 254, E(): 8.8e-11, (41.55% identity in 130 aa overlap). P9WF61 No virulence, detoxification, adaptation Q50625 P9WF61 P9WF61 NC_000962.3 Mycobrowser_v5 CDS 2824678 2825484 . + 0 Rv2509 Rv2509 Function unknown; possibly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv2509, (MTCY07A7.15), len: 268 aa. Probable ala-rich oxidoreductase, short-chain dehydrogenase/reductase, equivalent to O07709|MLCL383.36c|ML0429 dehydrogenase (putative oxidoreductase) from Mycobacterium leprae (268 aa), FASTA scores: opt: 1509, E(): 2.6e-84, (88.75% identity in 267 aa overlap). Also highly similar to others e.g. O86553|SC1F2.16c putative dehydrogenase from Streptomyces coelicolor (276 aa), FASTA scores: opt: 492, E(): 9.5e-23, (38.15% identity in 262 aa overlap); Q9I5R3|PA0658 probable short-chain dehydrogenase from Pseudomonas aeruginosa (266 aa), FASTA scores: opt: 472, E(): 1.5e-21, (37.8% identity in 246 aa overlap); AAK22120|CC0133 oxidoreductase (short-chain dehydrogenase/reductase family) from Caulobacter crescentus (266 aa), FASTA scores: opt: 428, E(): 6.9e-19, (35.8% identity in 243 aa overlap); etc. Also highly similar or similar to oxidoreductases from Mycobacterium tuberculosis e.g. Q10782|Rv1544|MTCY48.21 putative ketoacyl reductase (267 aa), FASTA scores: opt: 656, E(): 1.1e-32, (43.05% identity in 267 aa overlap). Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. I6Y9I3 No intermediary metabolism and respiration O06172 I6Y9I3 GO:0055114,GO:0016491 1.-.-.- I6Y9I3 NC_000962.3 Mycobrowser_v5 CDS 2834109 2835335 . - 0 Rv2518c ldtB Involved in peptidoglycan synthesis. Catalyzes the formation of 3->3 crosslinks between peptidoglycan subunits. Probable L,D-transpeptidase LdtB Rv2518c, (MTV009.03c), len: 408 aa. Probable ldtB, L,D-transpeptidase, highly similar to O07707|MLCL383.3 hypothetical 43.6 KDA protein from Mycobacterium leprae (407 aa), FASTA scores: opt: 2300, E(): 1.2e-130, (82.5% identity in 406 aa overlap); Q9CCU5|LPPS|ML0426 putative secreted protein from Mycobacterium leprae (404 aa), FASTA scores: opt: 2279, E(): 2.3e-129, (82.4% identity in 403 aa overlap); and Q9CB49|ML2446 possible lipoprotein from Mycobacterium leprae (441 aa), FASTA scores: opt: 736, E(): 8.4e-37, (35.6% identity in 399 aa overlap). Also similar to other proteins from several organisms e.g. Q9X811|SC6G10.26c putative secreted protein from Streptomyces coelicolor (424 aa), FASTA scores: opt: 867, E(): 1.1e-44, (32.25% identity in 403 aa overlap); Q9L1E8|SC3D11.14 putative lipoprotein from Streptomyces coelicolor (416 aa), FASTA scores: opt: 737, E(): 7e-37, (32.95% identity in 413 aa overlap); Q9KYV1|SCE22.11 putative lipoprotein from Streptomyces coelicolor (407 aa), FASTA scores: opt: 721, E(): 6.2e-36, (33.5% identity in 400 aa overlap). And similar to several hypothetical mycobacterial proteins e.g. Q11149|Y483_MYCTU|Rv0483|MT0501|MTCY20G9.09 (451 aa), FASTA scores: opt: 763, E(): 2.1e-38, (34.85% identity in 402 aa overlap). Has very long signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. Note that previously known as lppS I6Y9J2 No cell wall and cell processes O53223 I6Y9J2 I6Y9J2 NC_000962.3 Mycobrowser_v5 CDS 2838129 2839541 . - 0 Rv2522c Rv2522c Function unknown Conserved hypothetical protein Rv2522c, (MTV009.07c), len: 470 aa. Conserved hypothetical protein, equivalent, but longer 20 aa, to Q9X7E4|ML1193|MLCB458.08 from hypothetical 46.6 KDA protein Mycobacterium leprae (442 aa), FASTA scores: opt: 2521, E(): 4.1e-142, (86.35% identity in 440 aa overlap). Also similar to various proteins e.g. Q9K425|SCG22.20 putative peptidase from Streptomyces coelicolor (451 aa), FASTA scores: opt: 1097, E(): 1.1e-57, (42.5% identity in 451 aa overlap); Q9FCK3|2SC3B6.09 putative peptidase from Streptomyces coelicolor (470 aa), FASTA scores: opt: 669, E(): 2.8e-32, (34.2% identity in 462 aa overlap); Q98AF9|MLL6018 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (486 aa), FASTA scores: opt: 622, E(): 1.7e-29, (33.95% identity in 442 aa overlap); Q9RSU7|DR2025 ARGE/DAPE/ACY1 family protein from Deinococcus radiodurans (459 aa), FASTA scores: opt: 616, E(): 3.7e-29, (34.15% identity in 442 aa overlap); etc (include some similarity to hypothetical proteins from C. elegans and yeast). Alternative start possible at 6687 but then no RBS obvious. I6X4J0 No conserved hypotheticals O53227 I6X4J0 GO:0008152,GO:0005515,GO:0016787 I6X4J0 NC_000962.3 Mycobrowser_v5 CDS 2839538 2839930 . - 0 Rv2523c acpS Biosynthesis of fatty acids and lipids. Transfers the 4'-phosphopantetheine moiety from coenzyme A to a SER of acyl-carrier protein. Catalyzes the formation of holo-ACP, which mediates the transfer of acyl fatty-acid intermediates during the biosynthesis of fatty acids and lipids [catalytic activity: CoA + APO-[acyl-carrier protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] ]. holo-[acyl-carrier protein] synthase AcpS (holo-ACP synthase) (CoA:APO-[ACP]pantetheinephosphotransferase) (CoA:APO-[acyl-carrier protein]pantetheinephosphotransferase) Rv2523c, (MT2599, MTV009.08c), len: 130 aa. AcpS, holo-[Acyl Carrier Protein] synthase (see citation below), equivalent to Q9X7E3|ACPS_MYCLE|ML1192|MLCB458.07 holo-[acyl-carrier protein] synthase from Mycobacterium leprae (130 aa), FASTA scores: opt: 732, E(): 5.5e-42, (87.5% identity in 128 aa overlap). Also similar to others e.g. O86785|ACPS_STRCO|SC6G4.22c from Streptomyces coelicolor (123 aa), FASTA scores: opt: 204, E(): 6.6e-07, (36.7% identity in 139 aa overlap); Q9KPB6|VC2457 from Vibrio cholerae (126 aa), FASTA scores: opt: 163, E(): 0.00036, (32.55% identity in 129 aa overlap); P24224|ACPS_ECOLI|DPJ|B2563 from Escherichia coli strain K12 (125 aa), FASTA scores: opt: 151, E(): 0.0022, (30.55% identity in 131 aa overlap); etc. Belongs to the ACPS family. Acts on fas-I enzymes in C. glutamicum (See Chalut et al., 2006). P9WQD3 No lipid metabolism 3H7Q,3HQJ,3NE1,3NE3 P0A4W8 P9WQD3 GO:0006633,GO:0008897,GO:0009059,GO:0000287,GO:0005737 2.7.8.7 P9WQD3 NC_000962.3 Mycobrowser_v5 CDS 2851751 2852671 . - 0 Rv2528c mrr Involved in the acceptance of foreign DNA which is modified. Restricts both adenine- and cytosine-methylated DNA. Probable restriction system protein Mrr Rv2528c, (MTCY159.28), len: 306 aa. Probable mrr, restriction system protein, similar to other mrr proteins e.g. Q9RWS8|DR0587|MRR from Deinococcus radiodurans (306 aa), FASTA scores: opt: 776, E(): 4.2e-40, (40.45% identity in 309 aa overlap); P24202|MRR_ECOLI|B4351 from Escherichia coli strain K12 (304 aa), FASTA scores: opt: 647, E(): 2.9e-32, (35.25% identity in 309 aa overlap); Q9RX07|DR0508 from Deinococcus radiodurans (336 aa), FASTA scores: opt: 456, E(): 1.3e-20, (37.3% identity in 319 aa overlap); etc. I6Y9K2 No information pathways P95025 I6Y9K2 GO:0009307,GO:0004519,GO:0003677 I6Y9K2 NC_000962.3 Mycobrowser_v5 CDS 2858727 2859290 . - 0 Rv2534c efp Involved in peptide bond synthesis. Stimulate efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase. Probable elongation factor P Efp Rv2534c, (MTCY159.22), len: 187 aa. Probable efp, elongation factor P, equivalent to Q9CCS0|EFP|ML0522 elongation factor P from Mycobacterium leprae (187 aa), FASTA scores: opt: 1158, E(): 2.1e-67, (94.1% identity in 186 aa overlap). Also highly similar to many e.g. Q45288|EFP_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (187 aa), FASTA scores: opt: 843, E(): 3.4e-47, (69.5% identity in 187 aa overlap); Q9KXQ9|EFP from Streptomyces coelicolor (188 aa), FASTA scores: opt: 833, E(): 1.5e-46, (67.0% identity in 188 aa overlap); P49778|EFP_BACSU from Bacillus subtilis (185 aa), FASTA scores: opt: 607, E(): 4.6e-32, (47.8% identity in 182 aa overlap); P33398|EFP_ECOLI|B4147 from Escherichia coli strain K12 (187 aa), FASTA scores: opt: 503, E(): 1.8e-27, (42.3% identity in 182 aa overlap); etc. Belongs to the elongation factor P family. P9WNM3 No information pathways P64034 P9WNM3 GO:0003746,GO:0006414,GO:0005737 P9WNM3 NC_000962.3 Mycobrowser_v5 CDS 2859300 2860418 . - 0 Rv2535c pepQ Function unknown; hydrolyses peptides. Probable cytoplasmic peptidase PepQ Rv2535c, (MTCY159.21), len: 372 aa. Probable pepQ, cytoplasmic peptidase, equivalent to Q9CCS1|PEPQ|ML0521 putative cytoplasmic peptidase from Mycobacterium leprae (376 aa), FASTA scores: opt: 1954, E(): 1.1e-105, (82.7% identity in 376 aa overlap). Also similar to other peptidases e.g. P54518|YQHT_BACSU putative peptidase (belongs to peptidase family M24B) from Bacillus subtilis (353 aa), FASTA scores: opt: 808, E(): 1.6e-39, (39.65% identity in 368 aa overlap); Q9KXQ8|SC9C5.16c putative peptidase from Streptomyces coelicolor (368 aa), FASTA scores: opt: 803, E(): 3.2e-39, (43.15% identity in 380 aa overlap); Q9K950|BH2800 XAA-pro dipeptidase from Bacillus halodurans (355 aa), FASTA scores: opt: 801, E(): 4.1e-39, (39.45% identity in 365 aa overlap); etc. Note that second part of protein is similar to second part of MTCY49.29c|Rv2089c|MT2150|MTCY49.29c probable dipeptidase; belongs to peptidase family M24B from Mycobacterium tuberculosis (375 aa) (33.9% identity in 354 aa overlap) blast results: Score: 142 bits (359), E: 4e-33, Identities: 86/224 (38%), Positives: 119/224 (52%), Gaps: 4/224 (1%). Could be belong to peptidase family M24B. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). I6YDN6 No intermediary metabolism and respiration P95018 I6YDN6 GO:0009987,GO:0016805,GO:0046872,GO:0008235,GO:0006508,GO:0004177 3.4.13.9 I6YDN6 NC_000962.3 Mycobrowser_v5 CDS 2860452 2861144 . + 0 Rv2536 Rv2536 Unknown Probable conserved transmembrane protein Rv2536, (MTCY159.20c), len: 230 aa. Probable conserved transmembrane protein, equivalent to Q9CCS2|ML0520 putative membrane protein from Mycobacterium leprae (202 aa), FASTA scores: opt: 812, E(): 2e-41, (63.2% identity in 201 aa overlap). Also similar in part to Q9HMD5|VNG2594c from Halobacterium sp. strain NRC-1 (117 aa), FASTA scores: opt: 33.6, E(): 1.8, (33.6% identity in 116 aa overlap); and perhaps AAK65752|SMA1996 putative ABC transporter permease protein from Rhizobium meliloti (Sinorhizobium meliloti) plasmid pSymA (323 aa), FASTA scores: opt: 117, E(): 6.1, (30.6% identity in 121 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P95017 No cell wall and cell processes P95017 P95017 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2861148 2861591 . - 0 Rv2537c aroD Involved at the third step in the biosynthesis of chorismate within the biosynthesis of aromatic amino acids (the shikimate pathway). Catalyze a trans-dehydration via an enolate intermediate [catalytic activity: 3-dehydroquinate = 3-dehydroshikimate + H(2)O]. 3-dehydroquinate dehydratase AroD (AROQ) (3-dehydroquinase) (type II dhqase) Rv2537c, (MTCY159.19), len: 147 aa. AroD (alternate gene name: aroQ), 3-dehydroquinate dehydratase (see citation below), equivalent to Q9CCS3|AROD|ML0519 3-dehydroquinate dehydratase from Mycobacterium leprae (145 aa), FASTA scores: opt: 803, E(): 3.4e-46, (85.9% identity in 142 aa overlap). Also highly similar to many e.g. P96750|AROQ_CORPS from Corynebacterium pseudotuberculosis (146 aa), FASTA scores: opt: 559, E(): 4.1e-30, (61.05% identity in 136 aa overlap); Q9K949|BH2801 from Bacillus halodurans (145 aa), FASTA scores: opt: 453, E(): 4e-23, (52.15% identity in 138 aa overlap); P54517|AROQ_BACSU|YQHS from Bacillus subtilis (148 aa), FASTA scores: opt: 419, E(): 7.1e-21, (45.3% identity in 139 aa overlap); etc. Contains PS01029 Dehydroquinase class II signature. Belongs to the type-II 3-dehydroquinase family. P9WPX7 No intermediary metabolism and respiration 1H05,1H0R,1H0S,2DHQ,2Y71,2Y76,2Y77,2XB8,3N59,3N76,3N7A,3N86,3N87,3N8K,3N8N P0A4Z6 P9WPX7 GO:0009073,GO:0003855 4.2.1.10 P9WPX7 NC_000962.3 Mycobrowser_v5 CDS 2861588 2862676 . - 0 Rv2538c aroB Involved at the second step in the biosynthesis of chorismate within the biosynthesis of aromatic amino acids (the shikimate pathway) [catalytic activity: 7-phospho-3-deoxy-arabino-heptulosonate = 3-dehydroquinate + orthophosphate]. 3-dehydroquinate synthase AroB Rv2538c, (MTCY159.18), len: 362 aa. AroB, 3-dehydroquinate synthase (see citations below), equivalent to Q9CCS4|AROB_MYCLE|ML0518 3-dehydroquinate synthase from Mycobacterium leprae (361 aa), FASTA scores: opt: 2059, E(): 3.3e-117, (87.25% identity in 361 aa overlap). Also highly similar to many e.g. Q9KXQ6|AROB from Streptomyces coelicolor (363 aa), FASTA scores: opt: 1363, E(): 4e-75, (60.05% identity in 358 aa overlap); Q9X5D2|AROB_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (366 aa), FASTA scores: opt: 1154, E(): 1.7e-62, (50.95% identity in 359 aa overlap); P07639|AROB_ECOLI|B3389 from Escherichia coli strain K12 (362 aa), FASTA scores: opt: 771, E(): 2.4e-39, (40.6% identity in 345 aa overlap); etc. Belongs to the dehydroquinate synthase family. P9WPX9 No intermediary metabolism and respiration P0A4Z4 P9WPX9 GO:0009073,GO:0005737,GO:0003856 4.2.3.4 P9WPX9 NC_000962.3 Mycobrowser_v5 CDS 2868860 2869237 . + 0 Rv2548 vapC19 Unknown Possible toxin VapC19 Rv2548, (MTCY159.08c), len: 125 aa. Possible vapC19, toxin, part of toxin-antitoxin (TA) operon with Rv2547, contains PIN domain (See Arcus et al., 2005; Pandey and Gerdes, 2005). Similarity to others in Mycobacterium tuberculosis e.g. P71665|Rv1397c|MTCY21B4.14c hypothetical 15.0 KDA protein (133 aa), FASTA scores: opt: 265, E(): 7.1e-12, (42.3% identity in 123 aa overlap); and to Q97WY5|SSO1975 hypothetical protein from Sulfolobus solfataricus (125 aa), FASTA scores: opt: 131, E(): 0.018, (30.0% identity in 110 aa overlap); O52285|YLE hypothetical 14.9 KDA protein from Agrobacterium radiobacter (133 aa), FASTA scores: opt: 128, E(): 0.03, (32.8% identity in 125 aa overlap); etc. P9WF93 No virulence, detoxification, adaptation P95005 P9WF93 P9WF93 NC_000962.3 Mycobrowser_v5 CDS 2871206 2872015 . - 0 Rv2552c aroE Possibly involved at the fourth step in the biosynthesis of chorismate within the biosynthesis of aromatic amino acids (the shikimate pathway) [catalytic activity: shikimate + NADP(+) = 5-dehydroshikimate + NADPH]. Probable shikimate 5-dehydrogenase AroE (5-dehydroshikimate reductase) Rv2552c, (MTCY159.04), len: 269 aa. Probable aroE, shikimate 5-dehydrogenase, equivalent to Q9CCS7|AROE|ML0515 putative shikimate 5-dehydrogenase from Mycobacterium leprae (278 aa), FASTA scores: opt: 1452, E(): 1.8e-77, (81.5% identity in 270 aa overlap). Also highly similar, but longer 101 aa, to Q9KH59|AROE putative shikimate dehydrogenase (fragment) from Mycobacterium marinum (148 aa), FASTA scores: opt: 729, E(): 1.3e-35, (76.35% identity in 148 overlap); Q9F7W3|AROE from Mycobacterium ulcerans (148 aa), FASTA scores: opt: 718, E(): 5.9e-35, (75.7% identity in 148 aa overlap). And also similar to to others e.g. Q9KXQ2|AROE from Streptomyces coelicolor (255 aa), FASTA scores: opt: 572, E(): 2.8e-26, (43.4% identity in 251 aa overlap); Q98DY3|MLR4492 from Rhizobium loti (Mesorhizobium loti) (280 aa), FASTA scores: opt: 385, E(): 2.2e-15, (34.85% identity in 284 aa overlap); P74591|AROE_SYNY3|SLR1559 from Synechocystis sp. strain PCC 6803 (290 aa), FASTA scores: opt: 347, E(): 3.7e-13, (30.9% identity in 275 aa overlap); P15770|AROE_ECOLI|B3281 from Escherichia coli strain K12 (272 aa), FASTA scores: opt: 230, E(): 7.7e-08, (29.5% identity in 251 aa overlap); etc. Belongs to the shikimate dehydrogenase family. I6Y120 No intermediary metabolism and respiration P95001 I6Y120 GO:0005737,GO:0055114,GO:0004764 1.1.1.25 I6Y120 NC_000962.3 Mycobrowser_v5 CDS 2873771 2876485 . - 0 Rv2555c alaS Involved in translation mechanism [catalytic activity: ATP + L-alanine + tRNA(ala) = AMP + pyrophosphate + L-alanyl-tRNA (ala)]. Probable alanyl-tRNA synthetase AlaS (alanine--tRNA ligase) (alanine translase) (ALARS) Rv2555c, (MTCY318.01c-MTCY159.01), len: 904 aa. Probable alaS, alanyl-tRNA synthetase, equivalent to Q9CCT0|alas|ML0512 alanyl-tRNA synthetase from Mycobacterium leprae (908 aa), FASTA scores: opt: 5013, E(): 0, (84.65% identity in 907 aa overlap). Also highly similar to many e.g. Q9KXP9|alas from Streptomyces coelicolor (890 aa), FASTA scores: opt: 2159, E(): 3.8e-118, (53.45% identity in 907 aa overlap); Q9FFC7 Arabidopsis thaliana (Mouse-ear cress) (954 aa), FASTA scores: opt: 1963, E(): 1.1e-106, (41.1% identity in 925 aa overlap); Q9RS27|DR2300 from Deinococcus radiodurans (890 aa), FASTA scores: opt: 1352, E(): 4.1e-71, (38.05% identity in 915 aa overlap); etc. Belongs to class-II aminoacyl-tRNA synthetase family. P9WFW7 No information pathways O07438 P9WFW7 GO:0004813,GO:0006419,GO:0005737,GO:0046872,GO:0000049,GO:0005524 6.1.1.7 P9WFW7 NC_000962.3 Mycobrowser_v5 CDS 2878571 2879929 . - 0 Rv2559c Rv2559c Function unknown Conserved hypothetical alanine leucine valine rich protein Rv2559c, (MTCY9C4.09), len: 452 aa. Conserved hypothetical ala-, leu-, val-rich protein, equivalent to Q9CCT1|ML0510 hypothetical protein from Mycobacterium leprae (473 aa), FASTA scores: opt: 2411, E(): 3.9e-121, (83.4% identity in 452 aa overlap); O69490|O69490 hypothetical 47.1 KDA protein from Mycobacterium leprae (447 aa), FASTA scores: opt: 2406, E(): 6.9e-121, (83.95% identity in 448 aa overlap). Also highly similar to Q9KXP4|SC9C5.30c conserved ATP/GTP binding protein from Streptomyces coelicolor (451 aa), FASTA scores: opt: 1742, E(): 1.5e-85, (64.4% identity in 430 aa overlap); Q9RT67|DR1898 conserved hypothetical protein from Deinococcus radiodurans (434 aa), FASTA scores: opt: 1147, E(): 6.6e-54, (46.0% identity in 415 aa overlap); P45262|YCAJ_HAEIN|HI1590 hypothetical protein from Haemophilus influenzae (446 aa), FASTA scores: opt: 1140, E(): 1.6e-53, (42.5% identity in 428 aa overlap); etc. Also similar to Q50629|MTCY227.09|RUVB|Rv2592c|MT2669|MTCY227.09 holliday junction DNA helicase from Mycobacterium tuberculosis (344 aa), (30.1% identity in 296 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). P9WQN1 No conserved hypotheticals Q50739 P9WQN1 GO:0017111,GO:0005524 P9WQN1 NC_000962.3 Mycobrowser_v5 CDS 2883342 2884334 . + 0 Rv2564 glnQ Thought to be involved in active transport of glutamine across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable glutamine-transport ATP-binding protein ABC transporter GlnQ Rv2564, (MTCY9C4.04c), len: 330 aa. Probable glnQ, glutamine-transport ATP-binding protein ABC transporter (see citation below), highly similar to many e.g. Q9L0J9|SCD40A.12c putative ABC-transporter ATP-binding protein from Streptomyces coelicolor (246 aa), FASTA scores: opt: 598, E(): 2.5e-26, (46.35% identity in 218 aa overlap); O54136|SC2E9.11 from Streptomyces coelicolor (230 aa), FASTA scores: opt: 592, E(): 5.1e-26, (46.55% identity in 219 aa overlap); O29244|AF1018 from Archaeoglobus fulgidus (228 aa), FASTA scores: opt: 580, E(): 2.4e-25, (42.4% identity in 210 aa overlap); P75831|YBJZ_ECOLI|B0879 from Escherichia coli strain K12 (648 aa), FASTA scores: opt: 555, E(): 1.3e-23, (39.65% identity in 232 aa overlap); etc. Also highly similar to O53618|Rv0073|MTV030.17 ABC-transporter ATP-binding subunit from Mycobacterium tuberculosis (330 aa), FASTA scores: opt: 1782, E(): 4.7e-92, (83.65% identity in 330 aa overlap); etc. Shows some similarity to Q11040|YC81_MYCTU|MTCY50.01|Rv1281c|MT1318 hypothetical ABC transporter ATP-binding protein from Mycobacterium tuberculosis (612 aa) (32.9 % identity in 234 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00211 ABC transporters family signature, and PS00889 Cyclic nucleotide-binding domain signature 2. Belongs to the ATP-binding transport protein family (ABC transporters). P9WQI5 No cell wall and cell processes P63401 P9WQI5 GO:0016887,GO:0005952,GO:0008603,GO:0001932,GO:0006810,GO:0005524 P9WQI5 NC_000962.3 Mycobrowser_v5 CDS 2884611 2886362 . + 0 Rv2565 Rv2565 Function unknown Conserved protein Rv2565, (MTCY9C4.03c), len: 583 aa. Conserved protein, similar in part to Q9A6C3|CC2171 hypothetical protein from Caulobacter crescentus (610 aa), FASTA scores: opt: 765, E(): 2.8e-37, (32.15% identity in 575 aa overlap). C-terminus also highly similar to various bacterial proteins e.g. O34731|YLBK_BACSU hypothetical 28.3 KDA protein from Bacillus subtilis (260 aa), FASTA scores: opt: 386, E(): 2.2e-15, (33.05% identity in 245 aa overlap); CAC45997|SMC01003 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (321 aa), FASTA scores: opt: 352, E(): 2.5e-13, (29.65% identity in 280 aa overlap); Q9K9Q8|BH2587 hypothetical protein from Bacillus halodurans (275 aa), FASTA scores: opt: 334, E(): 2.5e-12, (33.7% identity in 175 aa overlap); etc. And shows similarity to C-terminal half of some eukaryotic proteins e.g. Q9R114|NTE neuropathy target esterase homolog from Mus musculus (Mouse) (1327 aa), FASTA scores: opt: 411, E(): 2.7e-16, (24.45% identity in 626 aa overlap); O60859 neuropathy target esterase from Homo sapiens (Human) (1327 aa), FASTA scores: opt: 410, E(): 3.1e-16, (24.1% identity in 627 aa overlap); Q9U969|SWS|CG2212 swiss cheese protein from Drosophila melanogaster (Fruit fly) (1425 aa), FASTA scores: opt: 401, E(): 1.1e-15, (27.75% identity in 544 aa overlap); etc. Also shows strong similarity to C-terminal half of O05884|Z95121|Rv3239c|MTY20B11.14c hypothetical 110.2 KDA protein from Mycobacterium tuberculosis (1048 aa), FASTA scores: opt: 648, E(): 3e-30, (36.55% identity in 572 aa overlap); and O69695|Rv3728|MTV025.076 putative two-domain membrane protein from Mycobacterium tuberculosis (1065 aa), FASTA scores: opt: 643, E(): 6e-30, (34.3% identity in 595 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). P9WIY7 No conserved hypotheticals P0A642 P9WIY7 GO:0004622,GO:0046470,GO:0005789 P9WIY7 NC_000962.3 Mycobrowser_v5 CDS 2886373 2889795 . + 0 Rv2566 Rv2566 Function unknown Long conserved protein Rv2566, (MTCY9C4.02c), len: 1140 aa. Long conserved protein, equivalent to O53120|ML2678 or MLCB1913.12 hypothetical protein from Mycobacterium leprae (1000 aa), FASTA scores: opt: 760, E(): 7.1e-38, (50.2% identity in 1128 aa overlap); and middle part equivalent to Q9ZB40 72.2 KDA protein (fragment) from Mycobacterium leprae (644 aa), FASTA scores: opt: 1017, E(): 1.5e-65, (45.65% identity in 655 aa overlap). Also highly similar to Q98HG6|MLL2877 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (1119 aa), FASTA scores: opt: 1413, E(): 3.7e-77, (52.4% identity in 1148 aa overlap); and N-terminus shows similarity with other proteins e.g. Q9HUN8|PA4926 hypothetical protein from Pseudomonas aeruginosa (311 aa), FASTA scores: opt: 278, E(): 3e-09, (29.95% identity in 284 aa overlap); and upstream ORF Q50652|YP69_MYCTU|Rv2569c|MT2645|MTCY227.32 conserved hypothetical protein from Mycobacterium tuberculosis (314 aa), FASTA scores: opt: 252, E(): 1.1e-07, (28.9% identity in 315 aa overlap). Equivalent to AAK46955 from Mycobacterium tuberculosis strain CDC1551 (1156 aa) but shorter 16 aa. Q50732 No conserved hypotheticals Q50732 Q50732 Q50732 NC_000962.3 Mycobrowser_v5 CDS 2889795 2892449 . + 0 Rv2567 Rv2567 Function unknown Conserved hypothetical alanine and leucine rich protein Rv2567, (MTCY227.34c, MTCY9C4.01c), len: 884 aa. Conserved hypothetical ala-, leu-rich protein, equivalent to O53121|ML2679|MLCB1913.13 hypothetical protein from Mycobacterium leprae (893 aa), FASTA scores: opt: 4326, E(): 0, (75.2% identity in 883 aa overlap); and similar to Q49755|YO11_MYCLE|ML0605|MLCL536.05c|U1937B|B1937_F1_4 hypothetical 61.8 KDA protein from Mycobacterium leprae (561 aa), FASTA scores: opt: 758, E(): 1.2e-38, (32.2% identity in 537 aa overlap). Also similar to others e.g. Q9HUN7|PA4927 hypothetical protein from Pseudomonas aeruginosa (830 aa), FASTA scores: opt: 1247, E(): 2.2e-68, (38.25% identity in 831 aa overlap); Q98HG7|MLL2876 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (803 aa), FASTA scores: opt: 937, E(): 1.9e-49, (32.15% identity in 828 aa overlap); CAC47419|SMC04057 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (802 aa), FASTA scores: opt: 900, E(): 3.4e-47, (30.85% identity in 852 aa overlap); etc. And similar to P71732|YO11_MYCTU|Rv2411c|MT2484|MTCY253.09 conserved hypothetical protein from Mycobacterium tuberculosis (551 aa), FASTA scores: opt: 781, E(): 4.6e-40, (33.75% identity in 495 aa overlap). P9WL97 No conserved hypotheticals Q50654 P9WL97 P9WL97 NC_000962.3 Mycobrowser_v5 CDS 2896013 2897803 . - 0 Rv2572c aspS Involved in translation mechanism [catalytic activity: ATP + L-aspartate + tRNA(asp) = AMP + pyrophosphate + L-aspartyl-tRNA(asp)]. Probable aspartyl-tRNA synthetase AspS (aspartate--tRNA ligase) (ASPRS) (aspartic acid translase) Rv2572c, (MTCY227.29), len: 596 aa. Probable aspS, aspartyl-tRNA synthetase, equivalent to P36429|SYD_MYCLE|ML0501|MLCB1259.19 aspartyl-tRNA synthetase from Mycobacterium leprae (589 aa), FASTA scores: opt: 3534, E(): 1.8e-215, (87.85% identity in 592 aa overlap). Also highly similar to many e.g. O67589|SYD_AQUAE|AQ_1677 from Aquifex aeolicus (603 aa), FASTA scores: opt: 1829, E(): 8.2e-108, (47.5% identity in 598 aa overlap); O32038|SYD_BACSU from Bacillus subtilis (592 aa), FASTA scores: opt: 1732, E(): 1.1e-101, (46.25% identity in 597 aa overlap); P21889|SYD_ECOLI|TLS|B1866 from Escherichia coli strain K12 (590 aa), FASTA scores: opt: 1588, E(): 1.3e-92, (47.35% identity in 581 aa overlap); etc. Contains PS00179 Aminoacyl-transfer RNA synthetases class-II signature 1. Belongs to class-II aminoacyl-tRNA synthetase family. P9WFW3 No information pathways Q50649 P9WFW3 GO:0004815,GO:0006422,GO:0005737,GO:0003676,GO:0005524 6.1.1.12 P9WFW3 NC_000962.3 Mycobrowser_v5 CDS 2899339 2900220 . + 0 Rv2575 Rv2575 Unknown Possible conserved membrane glycine rich protein Rv2575, (MTCY227.26c), len: 293 aa. Possible conserved membrane gly-rich protein, highly similar to hypothetical proteins e.g. Q9RR98|DR2596 conserved hypothetical protein from Deinococcus radiodurans (313 aa), FASTA scores: opt: 734, E(): 2.8e-38, (42.95% identity in 291 aa overlap); Q9HV81|PA4717 from Pseudomonas aeruginosa (297 aa), FASTA scores: opt: 641, E(): 1.5e-32, (43.35% identity in 300 aa overlap); Q98IA4|MLL2493 from Rhizobium loti (Mesorhizobium loti) (306 aa), FASTA scores: opt: 628, E(): 1e-31, (38.45% identity in 307 aa overlap); etc. Contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. P9WL85 No cell wall and cell processes P65019 P9WL85 GO:0016021 P9WL85 NC_000962.3 Mycobrowser_v5 CDS 2900918 2902507 . + 0 Rv2577 Rv2577 Unknown Conserved protein Rv2577, (MTCY227.24c), len: 529 aa. Conserved protein, showing similarity with various proteins from eukaryotes, in particular phosphatases, e.g. Q9SE01|pap purple acid phosphatase precursor from Glycine max (Soybean) (464 aa), FASTA scores: opt: 190, E(): 0.00026, (27.3% identity in 388 aa overlap); Q9SVP2|F18A5.90|AT4G13700 hypothetical 53.4 KDA protein from Arabidopsis thaliana (Mouse-ear cress) (474 aa), FASTA scores: opt: 280, E(): 6.6e-10, (27.2% identity in 331 aa overlap); Q9FK32 similarity to unknown protein from Arabidopsis thaliana (Mouse-ear cress) (529 aa), FASTA scores: opt: 249, E(): 6.2e-08, (25.3% identity in 435 aa overlap); Q12546|APHA acid phosphatase precursor from Aspergillus ficuum (614 aa), FASTA scores: opt: 207, E(): 2.9e-05, (22.95% identity in 458 aa overlap); etc. P9WL81 No conserved hypotheticals Q50644 P9WL81 GO:0046872,GO:0003993 NC_000962.3 Mycobrowser_v5 CDS 2902509 2903531 . - 0 Rv2578c Rv2578c Function unknown Conserved hypothetical protein Rv2578c, (MTCY227.23), len: 340 aa. Conserved hypothetical protein, highly similar to hypothetical proteins (conserved or not) e.g. Q9ZBJ3|SC9C7.17c from Streptomyces coelicolor (348 aa), FASTA scores: opt: 998, E(): 1.6e-55, (47.6% identity in 355 aa overlap); Q9I763|PA0069 from Pseudomonas aeruginosa (352 aa), FASTA scores: opt: 560, E(): 6e-28, (36.6% identity in 284 aa overlap); Q986C9|MLL7417 from Rhizobium loti (Mesorhizobium loti) (356 aa), FASTA scores: opt: 550, E(): 2.6e-27, (39.15% identity in 240 aa overlap); etc. P9WL79 No conserved hypotheticals P65023 P9WL79 GO:0003824,GO:0016021,GO:0046872,GO:0005886,GO:0051539 P9WL79 NC_000962.3 Mycobrowser_v5 CDS 2903639 2904541 . + 0 Rv2579 dhaA Generates a primary alcohol and halide from 1-haloalkane and H2O [catalytic activity: 1-haloalkane + H2O = a primary alcohol + halide]. Possible haloalkane dehalogenase DhaA (1-chlorohexane halidohydrolase) Rv2579, (MTCY227.22c), len: 300 aa. Possible dhaA, haloalkane dehalogenase, strictly equivalent to Q9XB14|ISO-RV2579 haloalkane dehalogenase (1-chlorohexane halidohydrolase) from Mycobacterium bovis (300 aa), FASTA scores: opt: 2075, E(): 7.1e-125, (99.35% identity in 300 aa overlap); note that only two residues, 120 and 293 are different. Also highly similar to others e.g. Q9ZER0|DHAAF haloalkane dehalogenase from Mycobacterium sp strain GP1 (307 aa), FASTA scores: opt: 842, E(): 2.3e-46, (44.95% identity in 298 aa overlap); Q53042|DHAA haloalkane dehalogenase from Rhodococcus rhodochrous, and Pseudomonas pavonaceae (293 aa), FASTA scores: opt: 837, E(): 4.5e-46, (44.6% identity in 298 aa overlap); etc. Note that this protein may also be a 1,3,4,6-tetrachloro-1,4-cyclohexadiene hydrolase, because also highly similar to P51698|LINB_PSEPA 1,3,4,6-tetrachloro-1,4-cyclohexadiene hydrolase from Pseudomonas paucimobilis (Sphingomonas paucimobilis) (see Nagata et al., 1993) (296 aa), FASTA scores: opt: 1494, E(): 6.8e-88, (69.5% identity in 295 aa overlap). Also shows some similarity with proteins from Mycobacterium tuberculosis e.g. Q50670|YM96_MYCTU|Rv2296|MT2353|MTCY339.14c putative haloalkane dehalogenase (300 aa), FASTA scores: opt: 302, E(): 5.3e-12, (30.85% identity in 295 aa overlap); and Q50600|YJ33_MYCTU|Rv1833c|MT1881|MTCY1A11.10 hypothetical 32.2 KDA protein (286 aa), FASTA scores: opt: 286, E(): 5.3e-11, (29.85% identity in 288 aa overlap). May belong to alpha/beta hydrolase fold family. Note that previously known as linB. P9WMR9 No intermediary metabolism and respiration 2O2H,2O2I,2QVB Q50642 P9WMR9 GO:0018786 3.8.1.5 P9WMR9 NC_000962.3 Mycobrowser_v5 CDS 2904821 2906092 . - 0 Rv2580c hisS Involved in translation mechamism [catalytic activity: ATP + L-histidine + tRNA(his) = AMP + pyrophosphate + L-histidyl-tRNA(his)]. Probable histidyl-tRNA synthetase HisS (histidine--tRNA ligase) (HISRS) (histidine--translase) Rv2580c, (MT2657, MTCY227.21), len: 423 aa. Probable hisS, histidyl-tRNA synthetase, equivalent to P46696|SYH_MYCLE|hiss|ML0494|MLCB1259.12|B1177_C3_248 histidyl-tRNA synthetase from Mycobacterium leprae (427 aa), FASTA scores: opt: 2380, E(): 2.1e-131, (85.85% identity in 417 aa overlap). Also highly similar to many e.g. Q9KXP2|hiss from Streptomyces coelicolor (425 aa), FASTA scores: opt: 1542, E(): 1.4e-82, (56.0% identity in 418 aa overlap); O32422|SYH_STAAU|hiss from Staphylococcus aureus (420 aa), FASTA scores: opt: 1135, E(): 7.4e-59, (44.9% identity in 412 aa overlap); P04804|SYH_ECOLI|hiss|B2514 from Escherichia coli strain K12 (423 aa), FASTA scores: opt: 1099, E(): 9.4e-57, (43.9% identity in 417 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to class-II aminoacyl-tRNA synthetase family. P9WFV5 No information pathways P67483 P9WFV5 GO:0005737,GO:0004821,GO:0006427,GO:0005524 6.1.1.21 P9WFV5 NC_000962.3 Mycobrowser_v5 CDS 2906089 2906763 . - 0 Rv2581c Rv2581c Involved in glyoxal pathway. Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid [catalytic activity: (S)-(2-hydroxyacyl)glutathione + H(2)O = glutathione + a 2-hydroxy acid anion]. Possible glyoxalase II (hydroxyacylglutathione hydrolase) (GLX II) Rv2581c, (MTCY227.20), len: 224 aa. Possible glyoxalase II, equivalent to Q49649|YP81_MYCLE|ML0493|MLCB1259.11|B1177_C3_247 hypothetical 23.9 KDA protein from Mycobacterium leprae (218 aa), FASTA scores: opt: 1264, E(): 7.8e-73, (82.0% identity in 222 aa overlap). Also highly similar to Q9KXP1|SC9C5.33c possible hydrolase from Streptomyces coelicolor (235 aa), FASTA scores: opt: 654, E(): 2.9e-34, (46.8% identity in 220 aa overlap); and similar to Q9CI24|YFCI hypothetical protein from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (210 aa), FASTA scores: opt: 360, E(): 9.9e-16, (35.0% identity in 217 aa overlap); AAK75726|SP1646 metallo-beta-lactamase superfamily protein from Streptococcus pneumoniae (209 aa), FASTA scores: opt: 320, E(): 3.3e-13, (35.85% identity in 198 aa overlap); AAK80229|CAC2272 predicted Zn-dependent hydrolase of metallo-beta-lactamase superfamily from Clostridium acetobutylicum (199 aa), FASTA scores: opt: 282, E(): 8e-11, (32.7% identity in 217 aa overlap); etc. Equivalent to AAK46971 from Mycobacterium tuberculosis strain CDC1551 (246 aa) but shorter 22 aa. Belongs to the glyoxalase II family. Cofactor: binds two zinc ions. P9WMW3 No virulence, detoxification, adaptation P9WMW3 GO:0046872,GO:0016787 3.-.-.- P9WMW3 NC_000962.3 Mycobrowser_v5 CDS 2906814 2907740 . + 0 Rv2582 ppiB PPIases accelerate the folding of proteins [catalytic activity: cis-trans isomerization of proline imidic peptide bonds inoligopeptides]. Probable peptidyl-prolyl cis-trans isomerase B PpiB (cyclophilin) (PPIase) (rotamase) (peptidylprolyl isomerase) Rv2582, (MTCY227.19c), len: 308 aa. Probable ppiB (alternate gene name: ppi), cyclophilin (peptidyl-prolyl cis-trans isomerase), equivalent to P46697|PPIB_MYCLE|PPI|ML0492|MLCB1259.10c|B1177_F3_97 probable peptidyl-prolyl cis-trans isomerase B from Mycobacterium leprae (295 aa), FASTA scores: opt: 1423, E(): 1.3e-66, (72.2% identity in 295 aa overlap). Also similar to others e.g. Q9KJG8|PPIB peptidyl-prolyl cis-trans isomerase from Streptomyces lividans (277 aa), FASTA scores: opt: 485, E(): 3.2e-18, (38.35% identity in 292 aa overlap); Q9KXP0|SC9C5.34 peptidyl-prolyl cis-trans isomerase from Streptomyces coelicolor (277 aa), FASTA scores: opt: 483, E(): 4.1e-18, (38.35% identity in 292 aa overlap); Q9RT72|DR1893 peptidyl-prolyl cis-trans isomerase from Deinococcus radiodurans (350 aa), FASTA scores: opt: 296, E(): 2.2e-08, (29.0% identity in 276 aa overlap); etc. Belongs to the cyclophilin-type PPIase family. P9WHW1 No information pathways Q50639 P9WHW1 GO:0006457,GO:0003755 5.2.1.8 P9WHW1 NC_000962.3 Mycobrowser_v5 CDS 2939012 2939962 . - 0 Rv2611c Rv2611c Catalyzes the acylation of the 6-position of the mannose residue linked to position 2 of the myo-inositol in phosphatylinositol mono- and DI-mannosides. Probable acyltransferase Rv2611c, (MTCY01A10.22), len: 316 aa. Probable acyltransferase , equivalent to O07148|MLCL581.15c|ML0453 hypothetical 35.4 KDA protein from Mycobacterium leprae (320 aa), FASTA scores: opt: 1529, E(): 5e-90, (71.45% identity in 312 aa overlap); and equivalent to Q9F7Y8 putative acyltransferase from Mycobacterium smegmatis (303 aa), FASTA scores: opt: 1464, E(): 6.5e-86, (72.15% identity in 291 aa overlap) (see citation below). Also highly similar to Q9L283|SCL2.16c putative acyltransferase from Streptomyces coelicolor (311 aa), FASTA scores: opt: 810, E(): 2.8e-44, (47.7% identity in 302 aa overlap); and similar to other acyltransferases e.g. Q9F0N3 acyltransferase from Campylobacter jejuni (295 aa), FASTA scores: opt: 207, E(): 6.4e-06, (20.45% identity in 220 aa overlap); Q9K379 acyltransferase (lipid a biosynthesis acyltransferase) from Campylobacter jejuni (295 aa), FASTA scores: opt: 203, E(): 1.1e-05, (20.0% identity in 220 aa overlap); etc. P9WMB5 No lipid metabolism O06203 P9WMB5 GO:0016021,GO:0009244,GO:0005886,GO:0008415 2.3.1.- P9WMB5 NC_000962.3 Mycobrowser_v5 CDS 2907826 2910198 . - 0 Rv2583c relA Involved in the metabolism of PPGPP (at the first step). In eubacteria PPGPP (guanosine 3'-diphosphate 5-'diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of PPPGPP which is then hydrolysed to form PPGPP [catalytic activity: ATP + GTP = AMP + guanosine 3'-diphosphate 5-'triphosphate]. Probable GTP pyrophosphokinase RelA (ATP:GTP 3'- pyrophosphotransferase) (PPGPP synthetase I) ((P)PPGPP synthetase) (GTP diphosphokinase) Rv2583c, (MTCY227.18), len: 790 aa. Probable relA, GTP pyrophosphokinase, equivalent to Q49640|RELA_MYCLE|ML0491|MLCB1259.09|B1177_C1_168 probable GTP pyrophosphokinase from Mycobacterium leprae (787 aa), FASTA scores: opt: 4834, E(): 0, (93.4% identity in 790 aa overlap). Also highly similar to others e.g. O87331|RELA_CORGL|RELA|rel from Corynebacterium glutamicum (Brevibacterium flavum) (760 aa), FASTA scores: opt: 3375, E(): 1.6e-196, (67.0% identity in 758 aa overlap); O85709|RELA_STRAT from Streptomyces antibioticus (841 aa), FASTA scores: opt: 3209, E(): 1.9e-186, (63.85% identity in 786 aa overlap); Q9KDH1|RELA|BH1242 from Bacillus halodurans (728 aa), FASTA scores: opt: 2195,E(): 3.8e-125, (45.65% identity in 714 aa overlap); etc. Belongs to the RELA / spot family. P9WHG9 No intermediary metabolism and respiration P66014 P9WHG9 GO:0005525,GO:0008728,GO:0016597,GO:0015969,GO:0016301,GO:0005524 2.7.6.5 P9WHG9 NC_000962.3 Mycobrowser_v5 tRNA 2969497 2969568 . - 0 MTB000035 valT - tRNA-Val (CAC) valT, tRNA-Val, anticodon cac, length = 72 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 2910229 2910900 . - 0 Rv2584c apt Involved in purine salvage. Catalyses a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis [catalytic activity: AMP + pyrophosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate]. Adenine phosphoribosyltransferase Apt (APRT) (AMP diphosphorylase) (AMP pyrophosphorylase) (transphosphoribosidase) Rv2584c, (MTCY227.17), len: 223 aa. Probable apt, adenine phosphoribosyltransferase, similar, but longer in N-terminus, to others e.g. O87330|APT_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (185 aa), FASTA scores: opt: 524, E(): 1.3e-24, (50.95% identity in 159 aa overlap); P52561|APT_STRCO from Streptomyces coelicolor (182 aa), FASTA scores: opt: 503, E(): 2.3e-23, (51.85% identity in 164 aa overlap); P47956|APT_MUSPA|APRT from Mus pahari (Shrew mouse) (180 aa), FASTA scores: opt: 419, E(): 2.5e-18, (44.7% identity in 170 aa overlap); P07672|P09993|P77121|APT_ECOLI|B0469 from Escherichia coli strain K12 (183 aa), FASTA scores: opt: 393, E(): 1.9e-18, (42.6% identity in 162 aa overlap); etc. Contains PS00103 Purine/ pyrimidine phosphoribosyl transferases signature, and PS00144 Asparaginase / glutaminase active site signature 1. Belongs to the purine/pyrimidine phosphoribosyltransferase family. Nearest initiation codon indicated by homology is TTG at 17426 or GTG at 17465. P9WQ07 No intermediary metabolism and respiration Q50637 P9WQ07 GO:0006168,GO:0005737,GO:0006166,GO:0003999 2.4.2.7 P9WQ07 NC_000962.3 Mycobrowser_v5 CDS 2911004 2912677 . - 0 Rv2585c Rv2585c Unknown Possible conserved lipoprotein Rv2585c, (MT2662, MTCY227.16), len: 557 aa. Possible conserved lipoprotein precursor, possibly attached to the membrane by a lipid anchor and substrate-binding protein involved in transport, equivalent to Q49646|YP85_MYCLE|ML0489|MLCB1259.07|B1177_C2_197 hypothetical lipoprotein precursor from Mycobacterium leprae (555 aa), FASTA scores: opt: 2812, E(): 9.8e-158, (78.95% identity in 546 aa overlap); and C-terminus highly similar to C-terminus of Q49638|DCIAE|B1177_C1_166 DCIAE protein from Mycobacterium leprae (344 aa), FASTA scores: opt: 1177, E(): 7.4e-62, (78.6% identity in 229 aa overlap). Also similar in part to various proteins, principally substrate-binding proteins, e.g. O87329|DCIAE dipeptide-binding protein from Corynebacterium glutamicum (Brevibacterium flavum) (502 aa), FASTA scores: opt: 614, E(): 1.2e-28, (30.7% identity in 427 aa overlap); Q9AKR0|OPPA|CAC49261 putative oligopeptide uptake ABC transporter periplasmic solute-binding protein precursor from Rhizobium meliloti (Sinorhizobium meliloti) (532 aa), FASTA scores: opt: 209, E(): 7.7e-05, (22.85% identity in 460 aa overlap); P76128|YDDS_ECOLI|B1487|P77769|P76874 putative ABC transporter periplasmic binding protein from Escherichia coli strain K12 (516 aa), FASTA scores: opt: 182, E(): 0.0029, (20.0% identity in 315 aa overlap); etc. P9WL77 No cell wall and cell processes Q50636 P9WL77 GO:0006810,GO:0005215,GO:0005886 P9WL77 NC_000962.3 Mycobrowser_v5 CDS 2912683 2914011 . - 0 Rv2586c secF Involved in protein export. Part of the prokaryotic protein translocation apparatus which comprise SECA, SECB, SECD, SECE, SECF, SECG and SECY. Probable protein-export membrane protein SecF Rv2586c, (MT2663, MTCY227.15), len: 442 aa. Probable secF, protein-export membrane protein (integral membrane protein) (see citation below), equivalent to P38386|SECF_MYCLE|SECF|ML0488|MLCB1259.06|B1177_C3_239 protein-export membrane protein from Mycobacterium leprae (471 aa), FASTA scores: opt: 1910, E(): 2.9e-104, (72.15% identity in 456 aa overlap). Also similar to others e.g. Q9AE06|SECF from Corynebacterium glutamicum (Brevibacterium flavum) (403 aa), FASTA scores: opt: 1198, E(): 9.8e-63, (47.1% identity in 399 aa overlap); Q53956|SECF_STRCO|SCL2.05c from Streptomyces coelicolor (373 aa), FASTA scores: opt: 670, E(): 6.4e-32, (39.25% identity in 400 aa overlap); Q55611|SECF_SYNY3|SLR0775 from Synechocystis sp. strain PCC 6803 (315 aa), FASTA scores: opt: 416, E(): 3.8e-17, (33.8% identity in 296 aa overlap); etc. Belongs to the SECD/SECF family, SECF family. Part of the prokaryotic protein translocation apparatus which comprise SECA|Rv3240c, SECD|Rv2587c, SECE|Rv0638, SECF, SECG|Rv1440 and SECY|Rv0732. P9WGN9 No cell wall and cell processes Q50635 P9WGN9 GO:0016021,GO:0006886,GO:0005886,GO:0015628,GO:0055085,GO:0015627,GO:0015450 P9WGN9 NC_000962.3 Mycobrowser_v5 CDS 2915846 2916193 . - 0 Rv2588c yajC Thought to be involved in secretion apparatus. Probable conserved membrane protein secretion factor YajC Rv2588c, (MTCY227.13), len: 115 aa. Probable yajC, secretion factor, a conserved membrane protein (see Braunstein & Belisle 2000), equivalent to Q49647|YP88_MYCLE|ML0486|MLCB1259.04|B1177_C3_235 hypothetical 12.8 KDA protein from Mycobacterium leprae (114 aa), FASTA scores: opt: 499, E(): 2.7e-26, (77.0% identity in 100 aa overlap). Also similar to other proteins e.g. Q9AE08 hypothetical 13.5 KDA protein from Corynebacterium glutamicum (Brevibacterium flavum) (121 aa), FASTA scores: opt: 222, E(): 5e-08, (39.8% identity in 103 aa overlap); Q9L292|SCL2.07c putative secreted protein from Streptomyces coelicolor (169 aa), FASTA scores: opt: 203, E(): 1.2e-06, (32.05% identity in 106 aa overlap); Q9CDT0|YWAB unknown protein from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (110 aa), FASTA scores: opt: 150, E(): 0.0026, (30.85% identity in 94 aa overlap); etc. P9WL75 No cell wall and cell processes P65025 P9WL75 GO:0005576 NC_000962.3 Mycobrowser_v5 CDS 2916360 2917709 . + 0 Rv2589 gabT Involved in 4-aminobutyrate (GABA) degradation pathway [catalytic activity: 4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate]. 4-aminobutyrate aminotransferase GabT (gamma-amino-N-butyrate transaminase) (GABA transaminase) (glutamate:succinic semialdehyde transaminase) (GABA aminotransferase) (GABA-at) Rv2589, (MTCY227.12c), len: 449 aa. Probable gabT, 4-aminobutyrate aminotransferase, equivalent to P40829|GABT_MYCLE|ML0485|MLCB1259.03c|B1177_F2_67 4-aminobutyrate aminotransferase (446 aa), FASTA scores: opt: 2468, E(): 4.5e-141, (83.75% identity in 449 aa overlap). Also highly similar to others e.g. O86823|GABT from Streptomyces coelicolor (444 aa), FASTA scores: opt: 1832, E(): 8e-103, (63.9% identity in 443 aa overlap); AAK79395|CAC1427 from Clostridium acetobutylicum (445 aa), FASTA scores: opt: 1283, E(): 8.4e-70, (45.75% identity in 433 aa overlap); Q9KE66|BH0991 from Bacillus halodurans (443 aa), FASTA scores: opt: 1224, E(): 2.9e-66, (44.55% identity in 431 aa overlap); etc. Contains PS00600 Aminotransferases class-III pyridoxal-phosphate attachment site. Belongs to class-III of pyridoxal-phosphate-dependent aminotransferases. Cofactor: pyridoxal phosphate. P9WQ79 No intermediary metabolism and respiration P63504 P9WQ79 GO:0003867,GO:0009448,GO:0030170,GO:0047298 2.6.1.22,2.6.1.19 P9WQ79 NC_000962.3 Mycobrowser_v5 CDS 2917871 2921377 . + 0 Rv2590 fadD9 Function unknown, but involved in lipid degradation. Probable fatty-acid-CoA ligase FadD9 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) Rv2590, (MTCY227.11c), len: 1168 aa. Probable fadD9, fatty-acid-CoA synthetase, highly similar to O69484|FADD9 (alias Q9CCT4|FADD9|ML0484 but longer 14 aa) putative acyl-CoA synthetase from Mycobacterium leprae (1174 aa), FASTA scores: opt: 5247, E(): 0, (68.0% identity in 1178 aa overlap). N-terminal (approximately 700 residues) similar to other long chain fatty acid ligases. And C-terminus highly similar to C-terminus of Q9XCF2|PSTB PSTB protein from Mycobacterium avium (2552 aa), FASTA scores: opt: 2083, E(): 8.4e-116, (40.8% identity in 1150 aa overlap) (and weak similarity on N-terminus). C-terminal part highly similar to polyketide synthases and peptides synthases (weak similarity on N-terminus) e.g. Q10896|Rv0101|MTCY251.20|NRP probable peptide synthetase from Mycobacterium tuberculosis (2512 aa), FASTA scores: opt: 1988, E(): 3.7e-110, (40.2% identity in 1181 aa overlap); etc. Contains PS00455 putative AMP-binding domain signature, and PS00061 Short-chain alcohol dehydrogenase family signature. Seems to belong to the ATP-dependent AMP-binding enzyme family, and to the short-chain dehydrogenases/reductases (SDR) family. Q50631 No lipid metabolism Q50631 Q50631 GO:0000036,GO:0048037,GO:0016874,GO:0008152,GO:0004043 6.2.1.- Q50631 NC_000962.3 Mycobrowser_v5 CDS 2924230 2924820 . - 0 Rv2593c ruvA forms a complex with RUVB. RUVA stimulates, in the presence of DNA, the weak ATPase activity of RUVB. The RUVA-RUVB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RUVAB is an helicase that mediates the holliday junction migration by localized denaturation and reanneling. Probable holliday junction DNA helicase RuvA Rv2593c, (MTCY227.08), len: 196 aa. Probable ruvA, Holliday junction binding protein (see citations below), equivalent to P40832|RUVA_MYCLE|ML0482|B1177_C2_188 holliday junction DNA helicase from Mycobacterium leprae (203 aa), FASTA scores: opt: 923, E(): 9.9e-50, (76.85% identity in 203 aa overlap). Also highly similar to others e.g. Q9L290|RUVA from Streptomyces coelicolor (201 aa) (201 aa), FASTA scores: opt: 549, E(): 8.2e-27, (47.55% identity in 204 aa overlap); Q9AE10|RUVA from Corynebacterium glutamicum (Brevibacterium flavum) (206 aa), FASTA scores: opt: 440, E(): 4e-20, (47.1% identity in 206 aa overlap); P08576|RUVA_ECOLI|B1861|Z2913|ECS2571 from Escherichia coli strains K12 and O157:H7 (203 aa), FASTA scores: opt: 312, E(): 2.8e-12, (34.85% identity in 201 aa overlap); etc. Belongs to the RuvA family. P9WGW3 No information pathways 2H5X,2ZTC,2ZTD,2ZTE P66744 P9WGW3 GO:0003677,GO:0006310,GO:0006281,GO:0009379,GO:0009432,GO:0009378,GO:0005524 3.6.4.12 P9WGW3 NC_000962.3 Mycobrowser_v5 CDS 2927990 2928391 . + 0 Rv2600 Rv2600 Unknown Probable conserved integral membrane protein Rv2600, (MTCY277.01c, MTV001.01), len: 133 aa. Probable conserved integral membrane protein, equivalent (but shorter 18 aa) to Q9K537|YQ00_MYCPA hypothetical protein RV2600 homolog from Mycobacterium paratuberculosis (151 aa), FASTA scores: opt: 543, E(): 4.2e-28, (62.9% identity in 132 aa overlap). Also some similarity with other hypothetical or membrane proteins e.g. Q9L090|SCC24.27c putative integral membrane protein from Streptomyces coelicolor (146 aa), FASTA scores: opt: 241, E(): 8.7e-09, (34.8% identity in 135 aa overlap); O58487|PH0773 hypothetical 15.0 KDA protein from Pyrococcus horikoshii (138 aa), FASTA scores: opt: 116, E(): 0.84, (34.35% identity in 96 aa overlap); etc. Equivalent to AAK46990 from Mycobacterium tuberculosis strain CDC1551 (152 aa) but shorter 19 aa. P9WFG5 No cell wall and cell processes P68915 P9WFG5 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2928388 2929959 . + 0 Rv2601 speE Involved in the biosynthesis of spermidine from arginine (at the fifth, last step). The activity is thought to be regulated mainly by the availability of decarboxylated S-adenosylmethionine [catalytic activity: S-adenosylmethioninamine + putrescine = 5'-methylthioadenosine + spermidine]. Probable spermidine synthase SpeE (putrescine aminopropyltransferase) (aminopropyltransferase) (SPDSY) Rv2601, (MTCI270.04c-MTV001.02), len: 523 aa. Probable speE, spermidine synthase, highly similar to many e.g. Q9L091|SCC24.26c from Streptomyces coelicolor (531 aa), FASTA scores: opt: 1493, E(): 1.3e-79, (48.45% identity in 514 aa overlap); Q9X8S2|SCH10.33c from Streptomyces coelicolor (554 aa), FASTA scores: opt: 1045, E(): 1.7e-53, (40.55% identity in 525 aa overlap); P09158|SPEE_ECOLI|B0121 from Escherichia coli strain K12 (287 aa), FASTA scores: opt: 368, E(): 2.9e-14, (30.5% identity in 272 aa overlap); etc. P9WGE5 No intermediary metabolism and respiration O33279 P9WGE5 GO:0004766,GO:0008295 2.5.1.16 P9WGE5 NC_000962.3 Mycobrowser_v5 CDS 2930805 2931560 . - 0 Rv2603c Rv2603c Function unknown Highly conserved protein Rv2603c, (MTCI270A.02), len: 251 aa. Highly conserved protein, equivalent to Q49645|YQ03_MYCLE|ML0475|U1177B|B1177_C2_181 hypothetical 26.6 KDA protein from Mycobacterium leprae (251 aa), FASTA scores: opt: 1514, E(): 2.2e-84, (92.45% identity in 251 aa overlap). Also highly similar to Q9L288|SCL2.11c hypothetical 26.8 KDA protein from Streptomyces coelicolor (250 aa), FASTA scores: opt: 1268, E(): 1.5e-69, (76.7% identity in 249 aa overlap); Q9AE12|YFCA hypothetical structural protein from Corynebacterium glutamicum (Brevibacterium flavum) (251 aa), FASTA scores: opt: 1231, E(): 2.6e-67, (72.9% identity in 251 aa overlap); O83487|Y474_TREPA|TP0474 hypothetical protein from Treponema pallidum (245 aa), FASTA scores: opt: 780, E(): 4.4e-40, (47.75% identity in 245 aa overlap); P24237|YEBC_ECOLI|B1864 protein YEBC from Escherichia coli strain K12 (246 aa), FASTA scores: opt: 776, E(): 7.6e-40, (47.8% identity in 249 aa overlap); etc. P9WGA5 No conserved hypotheticals P67177 P9WGA5 P9WGA5 NC_000962.3 Mycobrowser_v5 CDS 2931693 2932289 . - 0 Rv2604c snoP Possibly involved in the biosynthesis of pyridoxine/pyridoxal 5-phosphate biosynthesis Probable glutamine amidotransferase SnoP Rv2604c, (MTCY01A10.29, MTCI270A.01), len: 198 aa. Probable snoP, glutamine amidotransferase, equivalent (but shorter 21 aa) to Q49637|HISH|B1177_C1_149 HISH protein (belongs to the YFL060C/YAAE/HI1648 family) (alias Q9CCT5|ML0474 hypothetical protein 223 aa) from Mycobacterium leprae (219 aa), FASTA scores: opt: 1069, E(): 1.7e-60, (83.35% identity in 198 aa overlap). Also highly similar to hypothetical proteins or amidotransferases e.g. Q9L287|SCL2.12c hypothetical 21.5 KDA protein from Streptomyces coelicolor (202 aa), FASTA scores: opt: 702, E(): 2.3e-37, (56.75% identity in 192 aa overlap); P37528|YAAE_BACSU hypothetical 21.4 KDA protein from Bacillus subtilis (196 aa), FASTA scores: opt: 608, E(): 1.9e-31, (48.7% identity in 189 aa overlap); Q9KGN5|BH0023 amidotransferase from Bacillus halodurans (196 aa), FASTA scores: opt: 583, E(): 7.4e-30, (48.7% identity in 195 aa overlap); etc. Also some similarity with several proteins from Mycobacterium tuberculosis e.g. O06589|HIS5_MYCTU|Rv1602|MT1638|MTCY336.02c amidotransferase (206 aa), FASTA scores: opt: 154, E(): 0.00036, (30.6% identity in 193 aa overlap). Contains a Pfam match to entry PF01174 SNO glutamine amidotransferase family. Note possibly co-regulated with snzP (Rv2606c). P9WII7 No intermediary metabolism and respiration O06210 P9WII7 GO:0042823,GO:0016740,GO:0006543 2.6.-.- P9WII7 NC_000962.3 Mycobrowser_v5 CDS 2933171 2934070 . - 0 Rv2606c snzP Possibly involved in the biosynthesis of pyridoxine/pyridoxal 5-phosphate biosynthesis Possible pyridoxine biosynthesis protein SnzP Rv2606c, (MTCY01A10.27), len: 299 aa. Probable snzP, pyridoxine biosynthesis protein. Highly similar to O07145|YQ06_MYCLE|ML0450|MLCL581.12c possible pyridoxine biosynthesis protein from Mycobacterium leprae (307 aa), FASTA scores: opt: 1686, E(): 1.5e-95, (89.7% identity in 291 aa overlap). Also highly similar to several pyridoxine biosynthesis proteins and hypothetical proteins e.g. Q9L286|SCL2.13c hypothetical 32.2 KDA protein from Streptomyces coelicolor (303 aa), FASTA scores: opt: 1461, E(): 7.6e-82, (76.8% identity in 293 aa overlap); O14027|YEM4_SCHPO|SPAC29B12.04 putative stress-induced protein from Schizosaccharomyces pombe (Fission yeast) (296 aa), FASTA scores: opt: 1318, E(): 3.8e-73, (70.35% identity in 290 aa overlap); Q9UW83|PYROA protein involved in pyridoxine biosynthesis from Emericella nidulans (Aspergillus nidulans) (see citation below) (304 aa), FASTA scores: opt: 1288, E(): 2.6e-71, (67.9% identity in 302 aa overlap); etc. Contains Pfam match to entry PF01680, SOR_SNZ family. Contains PS01235 Uncharacterized protein family UPF0019 signature. Belongs to the SOR_SNZ family. Note possibly co-regulated with snoP (Rv2604c). P9WII9 No intermediary metabolism and respiration P60796 P9WII9 GO:0042823,GO:0016829 4.-.-.- P9WII9 NC_000962.3 Mycobrowser_v5 CDS 2934198 2934872 . + 0 Rv2607 pdxH Involved in biosynthesis of pyridoxine (vitamin B6) and pyridoxal phosphate. Oxidize PNP and PMP into pyridoxal 5'-phosphate (PLP)[catalytic activity: pyridoxamine 5'-phosphate + H(2)O + O(2) = pyridoxal 5'-phosphate + NH(3) + H(2)O(2)]. Probable pyridoxamine 5'-phosphate oxidase PdxH (PNP/PMP oxidase) (pyridoxinephosphate oxidase) (PNPOX) (pyridoxine 5'-phosphate oxidase) Rv2607, (MTCY01A10.26c), len: 224 aa. Probable pdxH, pyridoxinephosphate oxidase, equivalent to O33065|PDXH_MYCLE|ML2131|MLCB57.46 pyridoxamine 5'-phosphate oxidase from Mycobacterium leprae (219 aa), FASTA scores: opt: 1038, E(): 8.3e-61, (67.1% identity in 219 aa overlap). Also similar to others e.g. Q9I4S5|PDXH|PA1049 from Pseudomonas aeruginosa (215 aa), FASTA scores: opt: 608, E(): 1.1e-32, (49.55% identity in 218 aa overlap); Q9K3V7|SCD10.19c from Streptomyces coelicolor (234 aa), FASTA scores: opt: 600, E(): 3.9e-32, (42.3% identity in 234 aa overlap); P28225|PDXH_ECOLI|B1638 from Escherichia coli strain K12 (217 aa), FASTA scores: opt: 533, E(): 8.9e-28, (40.3% identity in 216 aa overlap); etc. Contains a match to Pfam entry PF01243 Pyridoxamine 5'-phosphate oxidase. Belongs to the pyridoxamine 5'-phosphate oxidase family. Cofactor: FMN. P9WIJ1 No intermediary metabolism and respiration 2A2J P65682 P9WIJ1 GO:0055114,GO:0004733,GO:0008615,GO:0010181 1.4.3.5 P9WIJ1 NC_000962.3 Mycobrowser_v5 CDS 2935046 2936788 . + 0 Rv2608 PPE42 Function unknown PPE family protein PPE42 Rv2608, (MTCY01A10.25c), len: 580 aa. PPE42, Member of the Mycobacterium tuberculosis PPE family, highly similar to many e.g. O06828|Rv1430|MTCY493.24c from Mycobacterium tuberculosis (528 aa), FASTA scores: opt: 1004, E(): 5.9e-48, (56.05% identity in 307 aa overlap). P9WHZ5 No PE/PPE Q79FC6 P9WHZ5 P9WHZ5 NC_000962.3 Mycobrowser_v5 CDS 2937865 2939001 . - 0 Rv2610c pimA Involved in the first mannosylation step in phosphatidylinositol mannoside biosynthesis (transfer of mannose residues onto PI, leading to the synthesis of phosphatidylinositol monomannoside). Alpha-mannosyltransferase PimA Rv2610c, (MTCY01A10.23), len: 378 aa. PimA, alpha-mannosyltransferase (see citations below), equivalent to O07147|MLCL581.14c|ML0452 putative glycosyltransferase from Mycobacterium leprae (374 aa), FASTA scores: opt: 2044, E(): 8.8e-118, (82.25% identity in 378 aa overlap). N-terminus (from aa 1 to 27) equivalent to Q9FY7 putative alpha-mannosyl transferase (fragment) from Mycobacterium smegmatis (27 aa), blastp scores: 57.4 bits (137), E(): 3e-8, Identities = 25/27 (92%), Positives = 27/27 (99%) (see citation below). Also highly similar to Q9L284|SCL2.15c putative sugar transferase from Streptomyces coelicolor (387 aa), FASTA scores: opt: 1222, E(): 1.8e-67, (52.95% identity in 376 aa overlap); and similar in part to various proteins e.g. Q9YA73|APE2066 long hypothetical N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein from Aeropyrum pernix (392 aa), FASTA scores: opt: 434, E(): 3e-19, (31.5% identity in 378 aa overlap); Q9UZA1|PAB0827 galactosyltransferase or LPS biosynthesis RFBU related protein from Pyrococcus abyssi (371 aa), FASTA scores: opt: 382, E(): 4.3e-16, (28.2% identity in 383 aa overlap); O26275|MTH173 LPS biosynthesis RFBU related protein from Methanothermobacter thermautotrophicus (382 aa), FASTA scores: opt: 372, E(): 1.8e-15, (28.4% identity in 391 aa overlap); etc. Shows also some similarity with O05313|Rv1212c|MTCI364.24c hypothetical 41.5 KDA protein from Mycobacterium tuberculosis (387 aa), FASTA scores: opt: 232, E(): 1.1e -07, (28.4% identity in 402 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). P9WMZ5 No lipid metabolism O06204 P9WMZ5 GO:0009405,GO:0043750,GO:0008654,GO:0005886,GO:0016021 2.4.1.57 P9WMZ5 NC_000962.3 Mycobrowser_v5 CDS 2940609 2941196 . - 0 Rv2613c Rv2613c Function unknown; but could be involved in lipid metabolism. Conserved protein Rv2613c, (MTCY01A10.20A), len: 195 aa. Conserved protein, equivalent to Q9CCU0|ML0455 hypothetical protein from Mycobacterium leprae (206 aa), FASTA scores: opt: 1074, E(): 7.4e-62, (84.7% identity in 196 aa overlap); and highly similar, but longer 18 aa, to O07150|MLCL581.17c hypothetical 20.7 KDA protein from Mycobacterium leprae (186 aa), FASTA scores: opt: 1038, E(): 1.4e-59, (89.7% identity in 175 aa overlap). Also highly similar to other hypothetical proteins (often Hit family member) e.g. Q9F7Z0 from Mycobacterium smegmatis (see citation below) (205 aa), FASTA scores: opt: 975, E(): 1.6e-55, (79.35% identity in 184 aa overlap); Q9L279|SCL2.20 from Streptomyces coelicolor (186 aa), FASTA scores: opt: 638, E(): 5.8e-34, (52.85% identity in 176 aa overlap); Q9YFX8|APE0122 from Aeropyrum pernix (184 aa), FASTA scores: opt: 515, E(): 4.4e-26, (45.9% identity in 159 aa overlap); etc. It seems the Rv2613c and downstream ORF Rv2612c|psgA1 are expressed from the same promoter (see citation below) and that Rv2613c should be involved in lipid metabolism. P9WMK9 No lipid metabolism 3ANO O06201 P9WMK9 GO:0005524,GO:0003877 2.7.7.53 P9WMK9 NC_000962.3 Mycobrowser_v5 CDS 2941189 2943267 . - 0 Rv2614c thrS Involved in translation mechanism [catalytic activity: ATP + L-threonine + tRNA(THR) = AMP + pyrophosphate + L-threonyl-tRNA(THR)]. Probable threonyl-tRNA synthetase ThrS (threonine-tRNA synthetase)(ThrRS) (threonine-tRNA ligase) Rv2614c, (MT2689, MTCY01A10.20), len: 692 aa. Probable thrS, threonyl-tRNA synthetase (Threonine--tRNA ligase), equivalent to O07151|SYT_MYCLE|THRS|ML0456|MLCL581.18c threonyl-tRNA synthetase from Mycobacterium leprae (702 aa), FASTA scores: opt: 3988, E(): 0, (84.05% identity in 702 aa overlap). Also highly similar to others e.g. Q9L278|THRS from Streptomyces coelicolor (658 aa), FASTA scores: opt: 1982, E(): 5.1e-114, (65.1% identity in 659 aa overlap); P56881|SYT_THETH|THRS from Thermus aquaticus (subsp. thermophilus) (659 aa), FASTA scores: opt: 1551, E(): 1.5e-87, (46.5% identity in 650 aa overlap); P00955|SYT_ECOLI from Escherichia coli (642 aa), FASTA scores: opt: 946, E(): 0, (40.7% identity in 612 aa overl ap); etc. Contains PS00339 Aminoacyl-transfer RNA synthetases class-II signature 2. Belongs to class-II aminoacyl-tRNA synthetase family. Cofactor: binds 1 zinc ion (by similarity). P9WFT5 No information pathways P67582 P9WFT5 GO:0005737,GO:0046872,GO:0004829,GO:0006435,GO:0005524 6.1.1.3 P9WFT5 NC_000962.3 Mycobrowser_v5 CDS 3769514 3769807 . - 0 Rv3355c Rv3355c Function unknown Probable integral membrane protein Rv3355c, (MTV004.12c), len: 97 aa. Probable integral membrane protein, equivalent to O32878|MLCB1779.16c|ML0675 hypothetical 9.6 KDA protein from Mycobacterium leprae (91 aa), FASTA scores: opt: 439, E(): 3.9e-23, (78.9% identity in 90 aa overlap). Identical, but with a gap, to O50377|Rv3346c|MTV004.02c hypothetical 8.9 KDA protein from Mycobacterium tuberculosis (85 aa), FASTA scores: opt: 413, E(): 2.1e-21, (85.55% identity in 97 aa overlap). Also some similarity to other proteins e.g. Q9K3J5|SC2A6.10 putative integral membrane protein from Streptomyces coelicolor (178 aa), FASTA scores: opt: 147, E(): 0.003, (31.25% identity in 80 aa overlap). O50384 No cell wall and cell processes O50384 O50384 NC_000962.3 Mycobrowser_v5 CDS 2948636 2949457 . + 0 Rv2622 Rv2622 Causes methylation Possible methyltransferase (methylase) Rv2622, (MTCY01A10.10c), len: 273 aa. Possible methyltransferase, similar in part to others e.g. AAK75664|SP1578 putative methyltransferase from Streptococcus pneumoniae (252 aa), FASTA scores: opt: 406, E(): 6.6e-18, (32.65% identity in 251 aa overlap); Q9F8B8 methyltransferase from Streptococcus agalactiae (254 aa), FASTA scores: opt: 381, E(): 2.3e-16, (31.75% identity in 252 aa overlap); Q9RJB6|SCF91.08 putative methyltransferase from Streptomyces coelicolor (231 aa), FASTA scores: opt: 159, E(): 0.0091, (33.1% identity in 151 aa overlap); etc. Also similar in part to several hypothetical proteins e.g. Q99YR0|SPY1582 hypothetical protein from Streptococcus pyogenes (251 aa), FASTA scores: opt: 397, E(): 2.3e-17, (36.3% identity in 248 aa overlap). I6XES4 No intermediary metabolism and respiration O06190 I6XES4 GO:0008168,GO:0008152 2.1.1.- I6XES4 NC_000962.3 Mycobrowser_v5 CDS 2949593 2950486 . + 0 Rv2623 TB31.7 Function unknown Universal stress protein family protein TB31.7 Rv2623, (MTCY01A10.09c), len: 297 aa. TB31.7, universal stress protein family protein, highly similar to hypothetical proteins from Mycobacterium tuberculosis e.g. Q10851|YK05_MYCTU|Rv2005c|MT2061|MTCY39.12 (295 aa), FASTA scores: opt: 1076, E(): 1.4e-60, (55.25% identity in 295 aa overlap); O53472|Rv2026c|MTV018.13c (294 aa), FASTA scores: opt: 988, E(): 4.8e-55, (51.5% identity in 295 aa overlap); Q10862|YJ96_MYCTU|Rv1996|MT2052|MTCY39.23c (317 aa), FASTA scores: opt: 688, E(): 4.1e-36, (45.1% identity in 315 aa overlap); etc. Also similar to several Streptomyces proteins e.g. Q9RIZ8|SCJ1.16c conserved hypothetical protein from Streptomyces coelicolor (294 aa), FASTA scores: opt: 407, E(): 2e-18, (32.65% identity in 303 aa overlap); and other bacterial hypothetical proteins e.g. Q9HPP5|VNG1536 from Halobacterium sp (147 aa), FASTA scores: opt: 180, E(): 0.00022, (31.65% identity in 139 aa overlap). Predicted possible vaccine candidate (See Zvi et al., 2008). Binds ATP. P9WFD7 No virulence, detoxification, adaptation 2JAX,3CIS O06189 P9WFD7 GO:0009405,GO:0006950,GO:0005524 P9WFD7 NC_000962.3 Mycobrowser_v5 CDS 2977231 2978658 . - 0 Rv2655c Rv2655c Unknown Possible PhiRv2 prophage protein Rv2655c, (MTCY441.24c), len: 475 aa. Hypothetical protein, possibly phiRv2 phage protein (putative primase-like protein) (see citation below). C-terminus similar to P22875|YXIS_SACER hypothetical 28.9 KDA protein (probably does not play a direct role in plasmid integration or excision) from Saccharopolyspora erythraea (Streptomyces erythraeus) plasmid pSE211 (263 aa), FASTA scores: opt: 389, E(): 2.7e-15, (33.45% identity in 269 aa overlap). Weak similarity in N-terminus to O06608|MTCY336.22|Rv1582c Probable phiRV1 phage protein from Mycobacterium tuberculosis (471 aa), FASTA scores: opt: 133, E(): 2.5, (36.0% identity in 75 aa overlap). I6Y1F0 No insertion seqs and phages P71952 I6Y1F0 I6Y1F0 NC_000962.3 Mycobrowser_v5 CDS 2957572 2958870 . + 0 Rv2631 Rv2631 Function unknown Conserved hypothetical protein Rv2631, (MTCY441.01, MTCY01A10.01c), len: 432 aa. Conserved hypothetical protein, highly similar to several conserved hypothetical proteins from various species e.g. O29399|AF0862 conserved hypothetical protein from Archaeoglobus fulgidus (482 aa), FASTA scores: opt: 1496, E(): 2.1e-80, (52.3% identity in 432 aa overlap) (has its N-terminus longer 30 aa); O27634|MTH1597 conserved protein from Methanothermobacter thermautotrophicus (488 aa), FASTA scores: opt: 1428, E(): 2.1e-76, (50.9% identity in 432 aa overlap); Q9YB37|APE1758 hypothetical 53.7 KDA protein APE1758 from Aeropyrum pernix (483 aa), FASTA scores: opt: 1422, E(): 4.6e-76, (49.3% identity in 432 aa overlap) (has its N-terminus longer 30 aa); etc. Equivalent to AAK47022 from Mycobacterium tuberculosis strain CDC1551 (432 aa). 3' part extended since first submission (+175 aa). P9WGW5 No conserved hypotheticals P71930 P9WGW5 P9WGW5 NC_000962.3 Mycobrowser_v5 CDS 2985283 2985753 . + 0 Rv2669 Rv2669 Acetylation, substrate unknown GCN5-related N-acetyltransferase Rv2669, (MTCY441.38), len: 156 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Similarity to several proteins e.g. Q9A6M0|CC2073 acetyltransferase (GNAT family) from Caulobacter crescentus (178 aa), FASTA scores: opt: 242, E(): 1.2e-09, (30.9% identity in 165 aa overlap); Q99RQ8|SA2159 hypothetical protein similar to transcription repressor of sporulation, septation and degradation paiA from Staphylococcus aureus subsp. aureus N315 (171 aa), FASTA scores: opt: 214, E(): 9.8e-08, (27.5% identity in 160 aa overlap); BAB58531|SAV2369 hypothetical 20.1 KDA protein from Staphylococcus aureus subsp. aureus Mu50 (171 aa), FASTA scores: opt: 214, E(): 9.8e-08, (27.5% identity in 160 aa overlap); P21340|PAIA_BACSU|O32112 protease synthase and sporulation from Bacillus subtilis (171 aa), FASTA scores: opt: 209, E(): 2.1e-07, (22.85% identity in 162 aa overlap); etc. P9WQG5 No intermediary metabolism and respiration P63425 P9WQG5 GO:0008152,GO:0008080 2.3.1.- P9WQG5 NC_000962.3 Mycobrowser_v5 CDS 2963586 2964242 . + 0 Rv2637 dedA Unknown Possible transmembrane protein DedA Rv2637, (MTCY441.07), len: 218 aa. Possible dedA, transmembrane protein, equivalent to Q49642|YQ37_MYCLE|ML0467|MLCL581.27|B1177_C2_172/B1177_C1_140 hypothetical 23.1 KDA protein (potential integral membrane protein, belongs to the DedA family) from Mycobacterium leprae (214 aa), FASTA scores: opt: 1160, E(): 4.4e-64, (82.75% identity in 209 aa overlap); and O69601|Y364_MYCLE|ML0287|MLCB4.30 hypothetical protein (potential integral membrane protein) (222 aa), FASTA scores: opt: 292, E(): 6.6e-11, (32.25% identity in 189 aa overlap). Also highly similar to other membrane proteins e.g. CAC42863|SCBAC36F5.27c putative integral membrane from Streptomyces coelicolor (211 aa), FASTA scores: opt: 837, E(): 2.6e-44, (59.2% identity in 201 aa overlap); Q55705|Y232_SYNY3|SLR0232 potential integral membrane protein from Synechocystis sp. strain PCC 6803 (218 aa), FASTA scores: opt: 415, E(): 1.9e-18, (37.85% identity in 206 aa overlap); Q9RV63|DR1167 DEDA protein from Deinococcus radiodurans (200 aa); P09548|DEDA_ECOLI|B2317|Z3579|ECS3201 DEDA protein (DSG-1 protein) from Escherichia coli strains K12 and O157:H7 (219 aa), blast scores: 178, E(): 1.8e-13, Identities = 53/175 (30%); etc. Also similar to O06314|Y364_MYCTU|Rv0364|MT0380|MTCY13E10.26 hypothetical 24.5 KDA protein (potential integral membrane protein) from Mycobacterium tuberculosis (227 aa), FASTA scores: opt: 293, E(): 5.8e-11, (35.85% identity in 184 aa overlap). Belongs to the DedA family. P9WP07 No cell wall and cell processes P63911 P9WP07 GO:0005886,GO:0016021 P9WP07 NC_000962.3 Mycobrowser_v5 CDS 2965026 2965358 . - 0 Rv2639c Rv2639c Unknown Probable conserved integral membrane protein Rv2639c, (MTCY441.09c), len: 110 aa. Probable conserved integral membrane protein, highly similar to many bacterial hypothetical or membrane proteins e.g. Q9X889|YE14_STRCO|SCE15.14 potential integral membrane protein from Streptomyces coelicolor (112 aa), FASTA scores: opt: 597, E(): 3.1e-31, (73.15% identity in 108 aa overlap); Q55939|Y793_SYNY3|SLL0793 potential integral membrane protein from Synechocystis sp. strain PCC 6803 (108 aa), FASTA scores: opt: 341, E(): 4.9e-15, (51.4% identity in 109 aa overlap); O31553|YFJF_BACSU potential integral membrane protein from Bacillus subtilis (109 aa), FASTA scores: opt: 334, E(): 1.4e-14, (47.5% identity in 109 aa overlap); etc. P9WFN9 No cell wall and cell processes P67146 P9WFN9 GO:0005886,GO:0016021 P9WFN9 NC_000962.3 Mycobrowser_v5 CDS 2965478 2965837 . - 0 Rv2640c Rv2640c Involved in transcriptional mechanism. Possible transcriptional regulatory protein (probably ArsR-family) Rv2640c, (MTCY441.10c), len: 119 aa. Possible transcriptional regulator, arsR family, highly similar to many e.g. Q9L1V5|SC4A9.07 putative ArsR-family transcriptional regulator from Streptomyces coelicolor (117 aa), FASTA scores: opt: 261, E(): 5.6e-10, (47.75% identity in 103 aa overlap); Q9X8X8|SCH35.28c putative transcriptional regulator from Streptomyces coelicolor (122 aa), FASTA scores: opt: 252, E(): 2.2e-09, (37.05% identity in 116 aa overlap); Q9L220|SC1A2.21 putative ArsR-family transcriptional from Streptomyces coelicolor (119 aa), FASTA scores: opt: 252, E(): 2.2e-09, (37.05% identity in 116 aa overlap); P77295|YGAV_ECOLI|B2667 hypothetical transcriptional regulator from Escherichia coli strain K12 (99 aa), FASTA scores: opt: 156, E(): 0.0023, (34.1% identity in 88 aa overlap); etc. Also similar to upstream ORF P71941|Rv2642|MTCY441.12 putative transcriptional regulatory protein from Mycobacterium tuberculosis (126 aa), FASTA scores: opt: 237, E(): 2e-08, (38.55% identity in 109 aa overlap). Contains helix-turn-helix motif at aa 59-80 (Score 1166, +3.16 SD). Belongs to the ArsR family of transcriptional regulators. I6Y1A7 No regulatory proteins P71939 I6Y1A7 GO:0006355,GO:0006350,GO:0003700,GO:0005622 I6Y1A7 NC_000962.3 Mycobrowser_v5 CDS 2966910 2968406 . + 0 Rv2643 arsC Involved in transport of arsenic compounds across the membrane (export): arsenic resistance by an export mechanism. Responsible for the translocation of the substrate across the membrane. Probable arsenic-transport integral membrane protein ArsC Rv2643, (MTCY441.13), len: 498 aa. Probable arsC, arsenical resistance transport integral membrane protein, highly similar or similar to others e.g. Q9L1X4|SC3D9.05 possible arsenic resistance membrane transport protein from Streptomyces coelicolor (368 aa), FASTA scores: opt: 1729, E(): 2.2e-96, (74.3% identity in 358 aa overlap); Q9X8Y0|SCH35.26 putative heavy metal resistance membrane protein from Streptomyces coelicolor (369 aa), FASTA scores: opt: 1729, E(): 2.2e-96, (73.8% identity in 359 aa overlap); Q06598|ACR3_YEAST|ACR3|YPR201W|P9677.2 arsenical-resistance protein from Saccharomyces cerevisiae (Baker's yeast) (404 aa), FASTA scores: opt: 591, E(): 4e-28, (36.6% identity in 380 aa overlap); etc. Belongs to the ACR3 family. I6X4W4 No cell wall and cell processes P71942 I6X4W4 GO:0016021,GO:0006470,GO:0004725,GO:0006814,GO:0008508 I6X4W4 NC_000962.3 Mycobrowser_v5 CDS 2979691 2980818 . - 0 Rv2659c Rv2659c Sequence integration. Integrase is necessary for integration of a phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome. Probable PhiRv2 prophage integrase Rv2659c, (MTCY441.28c), len: 375 aa. Probable integrase, phiRv2 phage protein: putative member of the phage integrase family of tyrosine recombinases (see Hatfull 2000), highly similar to others e.g. P22884|VINT_BPML5|33|int from Mycobacteriophage L5 (371 aa), FASTA scores: opt: 836, E(): 1.2e-44, (39.0% identity in 372 aa overlap); Q38361|VINT_BPMD2|33|int from Mycobacteriophage D29 (333 aa), FASTA scores: opt: 786, E(): 1.4e-41, (40.55% identity in 338 aa overlap); etc. Seems belongs to the 'phage' integrase family. P9WMB3 No insertion seqs and phages P71956 P9WMB3 GO:0015074,GO:0006310,GO:0003677 P9WMB3 NC_000962.3 Mycobrowser_v5 CDS 2985731 2986840 . - 0 Rv2670c Rv2670c Function unknown Conserved hypothetical protein Rv2670c, (MTCY441.39c), len: 369 aa. Conserved hypothetical protein, equivalent, but longer 164 aa, to O05683|MLC1351.22c hypothetical 17.3 KDA protein from Mycobacterium leprae (160 aa), FASTA scores: opt: 847, E(): 1.2e-45, (82.4% identity in 159 aa overlap). And highly similar to Q9X824|SC9B1.04c putative ATP/GTP-binding integral membrane protein from Streptomyces coelicolor (350 aa), FASTA scores: opt: 1169, E(): 2e-65, (56.85% identity in 343 aa overlap); and Q9RWB0|DR0759 conserved hypothetical protein from Deinococcus radiodurans (351 aa), FASTA scores: opt: 859, E(): 4e-46, (45.9% identity in 331 aa overlap). Also some similarity with other proteins e.g. P46442|YHCM_ECOLI|AAG58360|BAB37528 hypothetical protein from Escherichia coli strains K12 and O157:H7 (375 aa), FASTA scores: opt: 237, E(): 2.1e-07, (28.0% identity in 325 aa overlap); Q9JRK2|NMA1520|NMB1306 putative nucleotide-binding protein from Neisseria meningitidis (serogroup a and B) (383 aa), FASTA scores: opt: 221, E(): 2.1e-06, (27.8% identity in 356 aa overlap); Q9HVX7|PA4438 hypothetical protein from Pseudomonas aeruginosa (364 aa), FASTA scores: opt: 211, E(): 8.5e-06, (28.9% identity in 353 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). I6Y1G3 No conserved hypotheticals P71967 I6Y1G3 GO:0005524 I6Y1G3 NC_000962.3 Mycobrowser_v5 CDS 3001983 3003269 . + 0 Rv2685 arsB1 Thought to be involved in transport of arsenical compounds across the membrane (export): arsenic resistance by an export mechanism. Responsible for the translocation of the substrate across the membrane. Probable arsenic-transport integral membrane protein ArsB1 Rv2685, (MTCY05A6.06), len: 428 aa. Probable arsB1, arsenic-transport integral membrane protein, equivalent to P46838|AG45_MYCLE|ML1036 46 KDA probable integral membrane protein (antigen 45, a transmembrane protein related to arsenical pumps) from Mycobacterium leprae (429 aa), FASTA scores: opt: 2048, E(): 7.3e-120, (74.25% identity in 427 aa overlap); and downstream ORF O07186|YQ84_MYCTU|ARSA|Rv2684|MT2758|MTCY05A6.05 probable integral membrane protein ARSA from Mycobacterium tuberculosis (429 aa), FASTA scores: opt: 2154, E(): 1.9e-126, (76.8% identity in 427 aa overlap). Also highly similar to other proteins e.g. O59575|PH1912 hypothetical 46.0 KDA protein from Pyrococcus horikoshii (424 aa), FASTA scores: opt: 1075, E(): 1.9e-59, (43.55% identity in 427 aa overlap); Q9UY19|PAB1107 transport protein from Pyrococcus abyssi (425 aa), FASTA scores: opt: 1062, E(): 1.3e-58, (41.8% identity in 428 aa overlap); Q9KDI2|BH1231 hypothetical 46.0 KDA protein from Bacillus halodurans (428 aa), FASTA scores: opt: 993, E(): 2.4e-54, (39.55% identity in 430 aa overlap); etc. Belongs to the NADC/P/PHO87 family of transporters, P subfamily. Note that previously known as arsB. P9WPD7 No cell wall and cell processes O07187 P9WPD7 GO:0015137,GO:0015746,GO:0016021,GO:0005886,GO:0055085,GO:0015105 P9WPD7 NC_000962.3 Mycobrowser_v5 CDS 2989291 2990592 . + 0 Rv2673 aftC Involved in the biosynthesis of the mycobacterial cell wall arabinan Possible arabinofuranosyltransferase AftC Rv2673, (MTCY441.42), len: 433 aa. Possible aftC, arabinofuranosyltransferase (See Birch et al., 2008). Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). Possible conserved integral membrane protein, equivalent to MLC1351.25|ML1338 possible conserved integral membrane protein from Mycobacterium leprae (440 aa), FASTA scores: opt: 2410, E(): 5.3e-143, (82.05% identity in 434 aa overlap); and showing some similarity with Q9CBX0|ML1504 probable conserved membrane protein from Mycobacterium leprae (430 aa), FASTA scores: opt: 159, E(): 0.014, (24.4% identity in 340 aa overlap). Also similar to Q53873|SC6G4.11 putative integral membrane protein from Streptomyces coelicolor (411 aa), FASTA scores: opt: 383, E(): 1.4e-16, (29.6% identity in 422 aa overlap); and with weak similarity with P71061|YVFB hypothetical protein from Bacillus subtilis (396 aa), FASTA scores: opt: 136, E(): 0.36, (24.35% identity in 279 aa overlap); and BAB60134|TVG1014811 hypothetical protein from Thermoplasma volcanium (695 aa), FASTA scores: opt: 133, E(): 0.85, (26.45% identity in 280 aa overlap). Shows also some similarity with O06557|Rv1159|MTCI65.26 hypothetical 47.1 KDA protein from Mycobacterium tuberculosis (431 aa), FASTA scores: opt: 149, E(): 0.059, (22.45% identity in 410 aa overlap); and O53515|Rv2181|MTV021.14 putative membrane protein from Mycobacterium tuberculosis (427 aa), FASTA scores: opt: 129, E(): 1, (24.8% identity in 367 aa overlap). P9WMZ7 No cell wall and cell processes P71970 P9WMZ7 GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 2992634 2993992 . - 0 Rv2677c hemY Involved in heme and porphyrin biosynthesis (at the penultimate step). Catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX [catalytic activity: protoporphyrinogen-IX + O(2) = protoporphyrin-IX + H(2)O(2)]. Probable protoporphyrinogen oxidase HemY (protoporphyrinogen-IX oxidase) (protoporphyrinogenase) (PPO) Rv2677c, (MT2751, MTV010.01c), len: 452 aa. Probable hemY, protoporphyrinogen oxidase, equivalent to Q50008|PPOX_MYCLE|HEMY|ML1044 protoporphyrinogen oxidase from Mycobacterium leprae (451 aa), FASTA scores: opt: 2211, E(): 8.8e-118, (75.4% identity in 455 aa overlap). Also similar to others e.g. Q9RV99|DR1130 from Deinococcus radiodurans (462 aa), FASTA scores: opt: 523, E(): 2.7e-22, (29.8% identity in 453 aa overlap); O32434|PPOX_PROFR|HEMY from Propionibacterium freudenreichii shermanii (527 aa), FASTA scores: opt: 344, E(): 4e-12, (32.1% identity in 495 aa overlap); P32397|PPOX_BACSU|HEMY|HEMG from Bacillus subtilis (470 aa), FASTA scores: opt: 305, E(): 5.9e-10, (26.8% identity in 463 aa overlap); etc. Belongs to the protoporphyrinogen oxidase family. Cofactor: contains one FAD per homodimer. P9WMP1 No intermediary metabolism and respiration P0A5A7 P9WMP1 GO:0006783,GO:0055114,GO:0004729,GO:0005737 1.3.3.4 P9WMP1 NC_000962.3 Mycobrowser_v5 CDS 2993989 2995062 . - 0 Rv2678c hemE Involved in porphyrin biosynthesis [catalytic activity: uroporphyrinogen III = coproporphyrinogen + 4 CO(2)]. Probable uroporphyrinogen decarboxylase HemE (uroporphyrinogen III decarboxylase) (URO-D) (UPD) Rv2678c, (MTV010.02c), len: 357 aa. Probable hemE, uroporphyrinogen decarboxylase, equivalent to P46809|DCUP_MYCLE|heme|ML1043 uroporphyrinogen decarboxylase from Mycobacterium leprae (357 aa), FASTA scores: opt: 2017, E(): 8.2e-111, (83.75% identity in 357 aa overlap). Also highly similar to many e.g. O69861|DCUP_STRCO|heme|SC1C3.19 from Streptomyces coelicolor (355 aa), FASTA scores: opt: 1165, E(): 5.6e-61, (58.15% identity in 349 aa overlap); P32395|DCUP_BACSU|heme from Bacillus subtilis (353 aa), FASTA scores: opt: 859, E(): 4.5e-43, (44.1% identity in 356 aa overlap); Q9RV96|DCUP_DEIRA|heme|DR1133 from Deinococcus radiodurans (344 aa), FASTA scores: opt: 850, E(): 1.5e-42, (43.0% identity in 349 aa overlap); etc. Equivalent to AAK47067 from Mycobacterium tuberculosis strain CDC1551 (372 aa) but shorter 15 aa. Contains PS00907 Uroporphyrinogen decarboxylase signature 2. Belongs to the uroporphyrinogen decarboxylase family. P9WFE1 No intermediary metabolism and respiration O53231 P9WFE1 GO:0006779,GO:0004853,GO:0005737 4.1.1.37 P9WFE1 NC_000962.3 Mycobrowser_v5 CDS 2996105 2996737 . + 0 Rv2680 Rv2680 Function unknown Conserved protein Rv2680, (MTV010.04), len: 210 aa. Conserved protein, equivalent to Q50005|ML1041|U1764V hypothetical protein from Mycobacterium leprae (196 aa), FASTA scores: opt: 1136, E(): 9.7e-66, (83.95% identity in 193 aa overlap). Also similar to O69860|SC1C3.18c hypothetical 24.7 KDA protein from Streptomyces coelicolor (238 aa), FASTA scores: opt: 516, E(): 5.7e-26, (45.5% identity in 189 aa overlap); and similar in part to Q9I6V4|PA0178 probable two-component sensor from Pseudomonas aeruginosa (639 aa), FASTA scores: opt: 120, E(): 3.1, (33.05% identity in 115 aa overlap); and a few other proteins. Equivalent to AAK47069 from Mycobacterium tuberculosis strain CDC1551 (178 aa) but longer 32 aa; and N-terminus highly similar to N-terminus of AAK48352|MT3984 hypothetical 4.2 KDA protein from Mycobacterium tuberculosis strain CDC1551 (38 aa), FASTA scores: opt: 102, E(): 3.6, (62.05% identity in 29 aa overlap). Nucleotide position 2996194 in the genome sequence has been corrected, T:A resulting in V30V. O86317 No conserved hypotheticals O86317 O86317 O86317 NC_000962.3 Mycobrowser_v5 CDS 3010024 3010686 . + 0 Rv2692 ceoC Part of a potassium transport system. TRK system potassium uptake protein CeoC Rv2692, (MTCY05A6.13), len: 220 aa. CeoC (alternate gene names: trkA and trkB), TRK system potassium uptake protein (see citation below), highly similar to others e.g. O54141|SC2E9.16c from Streptomyces coelicolor (226 aa), FASTA scores: opt: 870, E(): 9.4e-48, (58.8% identity in 216 aa overlap); Q58505|TRKA_METJA|MJ1105 from Methanococcus jannaschii (218 aa), FASTA scores: opt: 361, E(): 9.7e-16, (29.8% identity in 218 aa overlap); O27333|TRKA_METTH|MTH1265 from Methanobacterium thermoautotrophicum (216 aa), FASTA scores: opt: 326, E(): 1.5e-13, (30.1% identity in 216 aa overlap); etc. Also similar to downstream orf O07193|CEOB|TRKA|Rv2691|MTCY05A6.12 TRK system potassium uptake protein from Mycobacterium tuberculosis (227 aa), FASTA scores: opt: 259, E(): 2.6e-09, (26.55% identity in 226 aa overlap). Contains a motif common to NAD+ binding pockets (see citation below). Belongs to the TrkA family. P9WFZ3 No cell wall and cell processes O07194 P9WFZ3 GO:0003824,GO:0008324,GO:0008152,GO:0006813 P9WFZ3 NC_000962.3 Mycobrowser_v5 CDS 3034909 3036102 . + 0 Rv2723 Rv2723 Unknown Probable conserved integral membrane protein Rv2723, (MTCY154.03), len: 397 aa. Probable conserved integral membrane protein, highly similar to others e.g. Q9Z503|SCC54.23c putative integral membrane export protein from Streptomyces coelicolor (333 aa), FASTA scores: opt: 883, E(): 2.4e-48, (46.4% identity in 332 aa overlap); Q9RD18|SCM1.25c putative integral membrane protein from Streptomyces coelicolor (316 aa), FASTA scores: opt: 865, E(): 3.1e-47, (47.55% identity in 324 aa overlap); P96554|Y319_MYXXA integral membrane protein (probable) from Myxococcus xanthus (319 aa), FASTA scores: opt: 626, E(): 3.4e-32, (34.65% identity in 323 aa overlap); P42601|YGJT_ECOLI|B3088 from Escherichia coli strain K12 integral membrane protein (probable) (321 aa), FASTA scores: opt: 541, E(): 7.7e-27, (35.1% identity in 279 aa overlap); etc. P9WG93 No cell wall and cell processes P0A614 P9WG93 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 3013683 3014147 . - 0 Rv2697c dut Involved in biosynthesis of thymidylate. This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA [catalytic activity: dUTP + H(2)O = dUMP + pyrophosphate]. Probable deoxyuridine 5'-triphosphate nucleotidohydrolase Dut (dUTPase) (dUTP pyrophosphatase) (deoxyuridine 5'-triphosphatase) (dUTP diphosphatase) (deoxyuridine-triphosphatase) Rv2697c, (MT2771, MTCY05A6.18c), len: 154 aa. Probable dut, deoxyuridine 5'-triphosphate nucleotidohydrolase (see citation below), equivalent to Q49992|DUT_MYCLE|ML1028 deoxyuridine 5'-triphosphate nucleotidohydrolase from Mycobacterium leprae (154 aa), FASTA scores: opt: 928, E(): 2.1e-51, (90.25% identity in 154 aa overlap). Also highly similar to others e.g. O54134|DUT_STRCO|SC2E9.09 from Streptomyces coelicolor (183 aa), FASTA scores: opt: 534, E(): 1.2e-26, (56.1% identity in 148 aa overlap); O66592|DUT_AQUAE|AQ_220 from Aquifex aeolicus (150 aa), FASTA scores: opt: 398, E(): 3.3e-18, (48.05% identity in 152 aa overlap); Q9X3X5|DUT_ZYMMO from Zymomonas mobilis (146 aa), FASTA scores: opt: 396, E(): 4.4e-18, (49.0% identity in 147 aa overlap); etc. Belongs to the dUTPase family. P9WNS5 No intermediary metabolism and respiration 1MQ7,1SIX,1SJN,1SLH,1SM8,1SMC,1SNF,2PY4,3H6D,3HZA,3I93,3LOJ P0A552 P9WNS5 GO:0046080,GO:0000287,GO:0004170 3.6.1.23 P9WNS5 NC_000962.3 Mycobrowser_v5 CDS 3015203 3015853 . + 0 Rv2700 Rv2700 Unknown Possible conserved secreted alanine rich protein Rv2700, (MTCY05A6.21), len: 216 aa. Possible secreted ala-rich protein, equivalent to Q4998|ML1025|U1764H possible secreted protein from Mycobacterium leprae (216 aa), FASTA scores: opt: 1198, E(): 1.2e-65, (82.4% identity in 216 aa overlap). Also showing some similarity with Q9AK75|2SCD60.08c conserved hypothetical protein from Streptomyces coelicolor (204 aa), FASTA scores: opt: 193, E(): 8.9e-05, (31.25% identity in 192 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). I6Y1I5 No cell wall and cell processes I6Y1I5 NC_000962.3 Mycobrowser_v5 CDS 3016858 3017655 . + 0 Rv2702 ppgK Catalyzes the phosphorylation of glucose using polyphosphate or ATP as the phosphoryl donor. GTP, UTP and CTP can replace ATP as phosphoryl donor [catalytic activity: (phosphate)(N) + D-glucose = (phosphate)(N-1) + D-glucose 6-phosphate]. Polyphosphate glucokinase PpgK (polyphosphate-glucose phosphotransferase) Rv2702, (MTCY05A6.23), len: 265 aa. PpgK, polyphosphate glucokinase (see citations below), equivalent, but shorter 60 aa, to Q49988|PPGK_MYCLE|ML1023|U1764FG polyphosphate glucokinase from Mycobacterium leprae (324 aa), FASTA scores: opt: 1411, E(): 5.6e-80, (82.8% identity in 262 aa overlap). Also highly similar (or just similar) to others e.g. Q9ADE8|PPGK from Streptomyces coelicolor (246 aa), FASTA scores: opt: 912, E(): 3e-49, (57.3% identity in 239 aa overlap); Q9AGV8|PPGK from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (277 aa), FASTA scores: opt: 890, E(): 7.5e-48, (57.75% identity in 239 aa overlap); P40184|GLK_STRCO|SC6E10.20c from Streptomyces coelicolor (317 aa), FASTA scores: opt: 233, E(): 3.2e-07, (31.3% identity in 163 aa overlap); etc. P9WIN1 No intermediary metabolism and respiration Q59568 P9WIN1 GO:0005737,GO:0047330,GO:0005524 2.7.1.63 P9WIN1 NC_000962.3 Mycobrowser_v5 CDS 3022461 3023432 . + 0 Rv2710 sigB The sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released. May control the regulons of stationary phase and general stress resistance. Seems to be regulated by sigh (Rv3223c product) and SIGE (Rv1221 product). Seems to regulate KATG|Rv1908c and the heat-shock response. RNA polymerase sigma factor SigB Rv2710, (MTCY05A6.31), len: 323 aa. SigB (formerly known as mysB), RNA polymerase sigma factor (see citations below), equivalent to Q59531|ML1014 RNA polymerase sigma factor from Mycobacterium leprae (319 aa), FASTA scores: opt: 1935, E(): 1.9e-109, (96.2% identity in 316 aa overlap). Also highly similar to others e.g. Q59553|MYSB from Mycobacterium smegmatis (319 aa), FASTA scores: opt: 1874, E(): 9.1e-106, (92.4% identity in 316 aa overlap); Q9ANT6|SIGB from Brevibacterium flavum (331 aa), FASTA scores: opt: 1525, E(): 9.9e-85, (78.9% identity in 303 aa overlap); Q60158|RPOV from Mycobacterium bovis (528 aa), FASTA scores: opt: 1246, E(): 9.3e-68, (62.85% identity in 315 aa overlap); etc. Contains sigma-70 factors family signatures 1 and 2 (PS00715 and PS00716). And contains possible helix-turn-helix motif at aa 282-303 (Score 1887, +5.61 SD). Belongs to the sigma-70 factor family. P9WGI5 No information pathways Q59563 P9WGI5 GO:0016987,GO:0003700,GO:0006352,GO:0006355 P9WGI5 NC_000962.3 Mycobrowser_v5 CDS 3833694 3834521 . - 0 Rv3415c Rv3415c Function unknown Conserved hypothetical protein Rv3415c, (MTCY78.14), len: 275 aa. Conserved hypothetical protein, equivalent to Q9CCV3|ML0383 hypothetical protein from Mycobacterium leprae (281 aa), FASTA scores: opt: 1278, E(): 4.2e-71, (73.5% identity in 279 aa overlap). Also some similarity with P71677|RIBD_MYCTU|RIBG|Rv1409|MT1453|MTCY21B4.26 riboflavin biosynthesis protein R (339 aa), FASTA scores: opt: 143, E(): 0.13, (28.25% identity in 184 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). I6YG27 No conserved hypotheticals I6YG27 I6YG27 NC_000962.3 Mycobrowser_v5 CDS 3025441 3026847 . + 0 Rv2713 sthA Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation [catalytic activity: NADPH + NAD(+) = NADP(+) + NADH]. Probable soluble pyridine nucleotide transhydrogenase SthA (STH) (NAD(P)(+) transhydrogenase [B-specific]) (nicotinamide nucleotide transhydrogenase) Rv2713, (MT2786, MTCY05A6.34), len: 468 aa. Probable sthA, soluble pyridine nucleotide transhydrogenase, highly similar to others e.g. Q983E2|MLR8366 from Rhizobium loti (Mesorhizobium loti) (481 aa), FASTA scores: opt: 1447, E(): 4.1e-78, (49.55% identity in 460 aa overlap); P27306|STHA_ECOLI|STH|UDHA|B3962 from Escherichia coli strain K12 (465 aa), FASTA scores: opt: 1267, E(): 1.7e-67, (43.05% identity in 462 aa overlap); O05139|STHA_PSEFL|STH from Pseudomonas fluorescens (463 aa), FASTA scores: opt: 1257, E(): 6.6e-67, (43.8% identity in 461 aa overlap); etc. Also highly similar to CAC46308|SMC00300 putative oxidoreductase protein from Rhizobium meliloti (Sinorhizobium meliloti) (467 aa), FASTA scores: opt: 1466, E(): 3e-79, (49.55% identity in 462 aa overlap). Shows some similarity to MTCY359.04, E(): 3.1e-08; MTCY210.05, E(): 3.4e-08. Contains ATP/GTP-binding site motif A (P-loop; PS00017). Belongs to the pyridine nucleotide-disulfide oxidoreductases class-I. Cofactor: FAD (by similarity). P9WHH5 No intermediary metabolism and respiration P66006 P9WHH5 GO:0003957,GO:0006739,GO:0045454,GO:0005737,GO:0055114,GO:0050660 1.6.1.1 P9WHH5 NC_000962.3 Mycobrowser_v5 CDS 3027065 3028039 . + 0 Rv2714 Rv2714 Function unknown Conserved alanine and leucine rich protein Rv2714, (MTCY05A6.35), len: 324 aa. Conserved ala-, leu-rich protein, equivalent to Q49847|ML1009|B2235_F1_6 hypothetical protein from Mycobacterium leprae (326 aa), FASTA scores: opt: 1881, E(): 5.8e-107, (89.7% identity in 320 aa overlap); and similar to Q49797|MLCB2533.03c|B2126_F1_36 hypothetical protein from Mycobacterium leprae (317 aa), FASTA scores: opt: 376, E(): 1.2e-15, (30.1% identity in 279 aa overlap); and Q9CC38|ML1306 hypothetical protein from Mycobacterium leprae (274 aa), FASTA scores: opt: 367, E(): 3.6e-15, (29.8% identity in 275 aa overlap). Also highly similar to Q9S2K6|SC7H2.11c hypothetical 34.2 KDA protein from Streptomyces coelicolor (312 aa), FASTA scores: opt: 770, E(): 1.4e-39, (40.9% identity in 286 aa overlap); and similar to Q9ADA5|SCI52.04 conserved hypothetical protein from Streptomyces coelicolor (333 aa), FASTA scores: opt: 386, E(): 3e-16, (29.05% identity in 296 aa overlap). Also similar to O33260|Rv2125|MTCY261.21 hypothetical protein from Mycobacterium tuberculosis (292 aa), FASTA scores: opt: 387, E(): 2.3e-16, (29.45% identity in 292 aa overlap). I6YA29 No conserved hypotheticals 2WAM O07213 I6YA29 I6YA29 NC_000962.3 Mycobrowser_v5 CDS 3031788 3032498 . + 0 Rv2720 lexA Involved in regulation of nucleotide excision repair and sos response. Represses a number of genes involved in the response to DNA damage (sos response), including RECA and LEXA. Has been shown to bind to the 14 bp palindromic sequence 5'-cgaacnnnngttcg-3'. In the presence of single-stranded DNA, RECA interacts with LEXA causing an autocatalytic cleavage which disrupts the DNA-binding part of LEXA, leading to derepression of the sos regulon and eventually DNA repair [catalytic activity: hydrolysis of ala-|-GLY bond in repressor LEXA]. Repressor LexA Rv2720, (MTCY05A6.41), len: 236 aa. LexA repressor (see citations below), equivalent to Q49848|LEXA_MYCLE|ML1003|B2235_F2_55 LEXA repressor from Mycobacterium leprae (217 aa), FASTA scores: opt: 1255, E(): 7.1e-70, (89.8% identity in 216 aa overlap). Also highly similar to others e.g. O69979|LEXA_STRCO|SC4H2.24c from Streptomyces coelicolor (234 aa), FASTA scores: opt: 1034, E(): 2.6e-56, (70.5% identity in 217 aa overlap); O86847|LEXA_STRCL from Streptomyces clavuligerus (239 aa), FASTA scores: opt: 1021, E(): 1.6e-55, (69.1% identity in 217 aa overlap); Q9KAD3|LEXA_BACHD from Bacillus halodurans (207 aa), FASTA scores: opt: 645, E(): 1.5e-32, (47.9% identity in 213 aa overlap); etc. Belongs to peptidase family S24; also known as the UMUD/LEXA family. Start changed since first submission (+19 aa). P9WHR7 No regulatory proteins Q50765 P9WHR7 GO:0006508,GO:0006281,GO:0006260,GO:0009432,GO:0045892,GO:0004252,GO:0006350,GO:0003677 3.4.21.88 P9WHR7 NC_000962.3 Mycobrowser_v5 CDS 3062816 3063634 . + 0 Rv2750 Rv2750 Function unknown; possibly involved in cellular metabolism. Probable dehydrogenase Rv2750, (MTV002.15), len: 272 aa. Probable dehydrogenase, highly similar to other dehydrogenases/reductases e.g. Q9L5X5|cox cholesterol oxidase from Nocardioides simplex (Arthrobacter simplex) (270 aa), FASTA scores: opt: 836, E(): 1.8e-43, (55.7% identity in 264 aa overlap); Q9RA05|LIMC carveol dehydrogenase from Rhodococcus erythropolis (277 aa), FASTA scores: opt: 792, E(): 8.6e-41, (48.55% identity in 274 aa overlap); Q9F5J1|SIM-NJ1|SIMD2 putative 3-keto-acyl-reductase from Streptomyces antibioticus (273 aa), FASTA scores: opt: 435, E(): 3.7e-19, (35.75% identity in 263 aa overlap); etc. Also highly similar to AAK44941MT0715 oxidoreductase (short-chain dehydrogenase/reductase family) from Mycobacterium tuberculosis strain CDC1551 (275 aa), FASTA scores: opt: 702, E(): 2.4e-35, (44.45% identity in 270 aa overlap); and similar to many other Mycobacterium tuberculosis dehydrogenases. P9WGS5 No intermediary metabolism and respiration O33292 P9WGS5 GO:0055114,GO:0016491 1.-.-.- P9WGS5 NC_000962.3 Mycobrowser_v5 CDS 3037427 3038914 . - 0 Rv2725c hflX Possibly a putative GTPase, modulating activity of HFLK and HFLC proteins. Probable GTP-binding protein HflX Rv2725c, (MTCY154.05c), len: 495 aa. Probable hflX (hfl for high frequency of lysogenization), GTP-binding protein ,equivalent to Q9CCC0|ML0997 (alias Q49843|HFLX but longer) possible ATP/GTP-binding protein from Mycobacterium leprae (488 aa), FASTA scores: opt: 2562, E(): 1.1e-133, (84.55% identity in 485 aa overlap). Also highly similar to many e.g. Q9XCC1 from Streptomyces fradiae (425 aa), FASTA scores: opt: 1280, E(): 3.2e-63, (57.7% identity in 423 aa overlap); P73965|HFLX|SLR1521 from Synechocystis sp. strain PCC 6803 (534 aa), FASTA scores: opt: 1028, E(): 2.8e-49, (44.7% identity in 414 aa overlap); P25519|HFLX_ECOLI|B4173 from Escherichia coli strain K12 (426 aa), FASTA scores: opt: 916, E(): 3.4e-43, (40.1% identity in 414 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). O33230 No intermediary metabolism and respiration O33230 O33230 GO:0016787,GO:0005622,GO:0005525 3.1.5.- O33230 NC_000962.3 Mycobrowser_v5 CDS 3039825 3040769 . - 0 Rv2727c miaA Catalyzes the first step in the biosynthesis of 2-methylthio-N6-(delta(2)-isopentenyl)-adenosine (ms[2]I[6]a]) adjacent to the anticodon of several tRNA species [catalytic activity: isopentenyl diphosphate + tRNA = pyrophosphate + tRNA containing 6-isopentenyladenosine]. Probable tRNA delta(2)-isopentenylpyrophosphate transferase MiaA (IPP transferase) (isopentenyl-diphosphate:tRNA isopentenyltransferase) (iptase) (IPPT) Rv2727c, (MTCY154.07c), len: 314 aa. Probable miaA, tRNA delta(2)-isopentenylpyrophosphate transferase, equivalent to P46811|MIAA_MYCLE|ML0995|B2235_C3_232 tRNA delta(2)-isopentenylpyrophosphate transferase from Mycobacterium leprae (311 aa), FASTA scores: opt: 1679, E(): 3.2e-89, (81.85% identity in 314 aa overlap). Also highly similar to many e.g. O69967|MIAA_STRCO|SC4H2.12 from Streptomyces coelicolor (312 aa), FASTA scores: opt: 1006, E(): 1.2e-50, (55.5% identity in 301 aa overlap); O31795|MIAA_BACSU from Bacillus subtilis (314 aa), FASTA scores: opt: 671, E(): 1.9e-31, (38.55% identity in 293 aa overlap);P16384|MIAA_ECOLI|TRPX|B4171 from Escherichia coli strain K12 and Shigella flexneri (316 aa), FASTA scores: opt: 565, E(): 2.3e-25, (35.2% identity in 307 aa overlap);etc. Contains PS00017 ATP/GTP-binding site motif A (P -loop). Belongs to the IPP transferase family. P9WJW1 No intermediary metabolism and respiration P65352 P9WJW1 GO:0016779,GO:0004811,GO:0008033,GO:0005524 2.5.1.75 P9WJW1 NC_000962.3 Mycobrowser_v5 CDS 3043026 3044378 . + 0 Rv2731 Rv2731 Function unknown Conserved alanine and arginine rich protein Rv2731, (MTCY174.11), len: 450 aa. Conserved ala-, arg-rich protein, highly similar in part to Q49849|B2235_F2_77 hypothetical protein from Mycobacterium leprae (266 aa), FASTA scores: opt: 368, E(): 1e-10, (73.5% identity in 83 aa overlap); and Q9KXN9|SC9C5.35 hypothetical 6.5 KDA protein (fragment) from Streptomyces coelicolor (58 aa), FASTA scores: opt: 214, E(): 0.00065, (51.7% identity in 58 aa overlap). Also similar to Q9L296|SCL2.01 hypothetical 37.4 KDA protein (fragment) from Streptomyces coelicolor (328 aa), FASTA scores: opt: 843, E(): 3.7e-33, (45.95% identity in 296 aa overlap) (but N-terminus shorter); and shows some similarity with other proteins e.g. Q26938 kinetoplast-associated protein (KAP) from Trypanosoma cruzi (1052 aa), FASTA scores: opt: 223, E(): 0.0022, (30.3% identity in 297 aa overlap). Start site chosen by RBS and to avoid overlap, although there are several other possible start sites further upstream. I6XF60 No conserved hypotheticals O33236 I6XF60 I6XF60 NC_000962.3 Mycobrowser_v5 CDS 3044986 3046524 . - 0 Rv2733c Rv2733c Unknown Conserved hypothetical alanine, arginine-rich protein Rv2733c, (MTCY154.13c), len: 512 aa. Conserved hypothetical ala-, arg-rich protein. Similar to other hypothetical proteins from a range of organisms e.g. Y195_MYCLE|Q49842 hypothetical 56.0 kDa protein b2235_c2_195 from Mycobacterium leprae (516 aa), FASTA scores: opt: 2689, E(): 0, (80.4% identity in 509 aa overlap). P9WK05 No conserved hypotheticals P67085 P9WK05 GO:0005737,GO:0046872,GO:0006400,GO:0016740,GO:0051539 2.-.-.- P9WK05 NC_000962.3 Mycobrowser_v5 CDS 3052023 3053189 . - 0 Rv2739c Rv2739c Function unknown; probably involved in cellular metabolism. Possible alanine rich transferase Rv2739c, (MTV002.04c), len: 388 aa. Possible ala-rich transferase, equivalent to Q49841|ML0985|MLCB33.02c|U2235C possible glycosyltransferase from Mycobacterium leprae (392 aa), FASTA scores: opt: 2112, E(): 5.1e-114, (80.95% identity in 388 aa overlap). Shows some similarity with other transferases e.g. Q9S1V2|SCJ4.21 putative glycosyl transferase from Streptomyces coelicolor (407 aa), FASTA scores: opt: 290, E(): 2e-09, (27.75% identity in 382 aa overlap); Q9RYI3|DRA0329 putative glycosyltransferase from Deinococcus radiodurans (418 aa), FASTA scores: opt: 267, E(): 4.3e-08, (29.05% identity in 396 aa overlap); P96560|GTFC glycosyltransferase from Amycolatopsis orientalis (409 aa), FASTA scores: opt: 253, E(): 2.7e-07, (27.75% identity in 418 aa overlap); etc. Equivalent to AAK47130 from Mycobacterium tuberculosis strain CDC1551 (420 aa) but shorter 32 aa. O33282 No intermediary metabolism and respiration O33282 O33282 GO:0005975,GO:0030259,GO:0016758,GO:0030246 2.-.-.- O33282 NC_000962.3 Mycobrowser_v5 CDS 3068189 3068464 . - 0 Rv2755c hsdS.1 Implicated in restriction/modification of DNA. Component of type I restriction/modification system. It's possible that the M and S subunits together form a methyltransferase (MTASE) that methylates two adenine residues in complementary strands of bipartite DNA recognition sequence. Possible type I restriction/modification system specificity determinant (fragment) HsdS.1 (S protein) Rv2755c, (MTV002.20c), len: 91 aa. Possible hsdS.1, fragment of type I restriction/modification system specificity determinant (S protein), similar to the N-terminus of other hsdS proteins e.g. O34140|HSDS from Klebsiella pneumoniae (439 aa), FASTA scores: opt: 303, E(): 2.1e-13, (46.65% identity in 90 aa overlap); P72419|sty|SBLI from Salmonella typhimurium (434 aa), FASTA scores: opt: 278, E(): 1.1e-11, (47.65% identity in 86 aa overlap); and Q9P9X9|XF2741 from Xylella fastidiosa (412 aa), FASTA scores: opt: 144, E(): 0.015, (31.7% identity in 82 aa overlap). Also some similarity with O33303|Rv2761c|MTV002.26c|HSDS possible type I restriction/modification system specificity determinant from Mycobacterium tuberculosis (364 aa), FASTA scores: opt: 145, E(): 0.012, (29.9% identity in 87 aa overlap). Note that previously known as hsdS'. I6XF84 No information pathways I6XF84 NC_000962.3 Mycobrowser_v5 CDS 3057251 3058063 . - 0 Rv2744c 35kd_ag Unknown Conserved 35 kDa alanine rich protein Rv2744c, (MTV002.09c), len: 270 aa. 35kd_ag, conserved ala-rich protein 35-kd antigen (see O'Connor et al., 1990). N-terminal part is equivalent to Q49840|MLCB33.06c|B2235_C2_187 hypothetical protein from Mycobacterium leprae (167 aa), FASTA scores: opt: 789, E(): 3.4e-35, (85.05% identity in 147 aa overlap); and C-terminal part equivalent to Q49845|MLCB33.05c|B2235_C3_214 hypothetical protein from Mycobacterium leprae (114 aa), FASTA scores: opt: 465, E(): 3.6e-18, (65.8% identity in 114 aa overlap); note that these two proteins from Mycobacterium leprae are adjacent. Shows some similarity with Q55707||Y617_SYNY3|SLL0617 hypothetical 28.9 KDA protein from Synechocystis sp. strain PCC 6803 (267 aa), FASTA scores: opt: 155, E(): 0.19, (23.4% identity in 252 aa overlap); and C-terminus of Q9L4N1|EMM M protein from Streptococcus equisimilis (592 aa), FASTA scores: opt: 165, E(): 0.11, (23.45% identity in 260 aa overlap). C-terminus also similar to AAK45945|MT1676 conserved hypothetical protein from Mycobacterium tuberculosis strain CDC1551 (85 aa), FASTA scores: opt: 159, E(): 0.047, (50.9% identity in 55 aa overlap). Predicted possible vaccine candidate (See Zvi et al., 2008). P9WHP5 No conserved hypotheticals P0C5C4 P9WHP5 GO:0005737 P9WHP5 NC_000962.3 Mycobrowser_v5 CDS 3058602 3059231 . - 0 Rv2746c pgsA3 Involved in acidic phospholipid biosynthesis. This protein probably catalyzes the committed step to the synthesis of the acidic phospholipids [catalytic activity: CDP-diacylglycerol + glycerol-3-phosphate = CMP + 3-(3-phosphatidyl)-glycerol 1-phosphate]. Probable PGP synthase PgsA3 (CDP-diacylglycerol--glycerol-3-phosphate 3- phosphatidyltransferase) (phosphatidylglycerophosphate synthase) Rv2746c, (MTV002.11c), len: 209 aa. Probable pgsA3, PGP synthase (see citation below), transmembrane protein, equivalent, but longer 19 aa, to Q49839|O08087|PGSA|ML0979 PGSA from Mycobacterium leprae (193 aa), FASTA scores: opt: 925, E(): 3.7e-53, (77.15% identity in 188 aa overlap). Also highly similar to O86813|PGSA phosphatidylglycerophosphate synthase from Streptomyces coelicolor (263 aa), FASTA scores: opt: 692, E(): 6.6e-38, (57.85% identity in 185 aa overlap) (has its N-terminus longer); and similar to others (generally with N-terminus shorter) e.g. Q99XI0|PGSA|SPY2196 phosphatidylglycerophosphate synthase from Streptococcus pyogenes (180 aa), FASTA scores: opt: 368, E(): 5.4e-17, (39.9% identity in 168 aa overlap); Q9ZE96|PGSA_RICPR|PGSA|RP049 CDP-diacylglycerol--glycerol-3-phosphate 3- phosphatidyltransferase from Rickettsia prowazekii (181 aa), FASTA scores: opt: 343, E(): 2.3e-15, (40.1% identity in 172 aa overlap); P06978|PGSA_ECOLI|PGSA|B1912|Z3000|ECS2650 CDP-diacylglycerol--glycerol-3-phosphate 3- phosphatidyltransferase from Escherichia coli strains K12 and O157:H7 (181 aa), FASTA scores: opt: 322, E(): 5.3e-14, (34.45% identity in 180 aa overlap); etc. Also some similarity to PGSA2|Rv1822|MTCY1A11.21c probable CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase from Mycobacterium tuberculosis (209 aa), FASTA score: (27.1% identity in 166 aa overlap). Contains PS00379 CDP-alcohol phosphatidyltransferases signature. Belongs to the CDP-alcohol phosphatidyltransferase class-I family. P9WPG3 No lipid metabolism O33288 P9WPG3 GO:0016021,GO:0008654,GO:0005886,GO:0008444 2.7.8.5 P9WPG3 NC_000962.3 Mycobrowser_v5 CDS 3068461 3070083 . - 0 Rv2756c hsdM Implicated in methylation of DNA. Component of type I restriction/modification system. It is possible that the M and S subunits together form a methyltransferase (MTASE) that methylates two adenine residues in complementary strands of bipartite DNA recognition sequence. Possible type I restriction/modification system DNA methylase HsdM (M protein) (DNA methyltransferase) Rv2756c, (MTV002.21c), len: 540 aa. Possible hsdM, type I restriction/modification system DNA methylase (M protein), highly similar to others e.g. Q9P9X8|XF2742 from Xylella fastidiosa (519 aa), FASTA scores: opt: 1613, E(): 1.9e-96, (52.3% identity in 543 aa overlap); O34139|HSDM from Klebsiella pneumoniae (539 aa), FASTA scores: opt: 1267, E(): 4.4e-74, (45.9% identity in 549 aa overlap); P72418|sty|SBLI|HSDM from Salmonella typhimurium (539 aa), FASTA scores: opt: 1263, E(): 8e-74, (45.7% identity in 549 aa overlap); etc. Possible alternative start site (GTG) overlapping with termination codon of previous ORF 90 bp upstream. Note that the corresponding endonuclease (M protein) does not appear to be present in Mycobacterium tuberculosis. O33298 No information pathways O33298 O33298 GO:0006306,GO:0008170,GO:0003677 2.1.1.- O33298 NC_000962.3 Mycobrowser_v5 CDS 3071546 3072640 . - 0 Rv2761c hsdS Implicated in restriction/modification of DNA. Component of type I restriction/modification system. It is thought that the M and S subunits together form a methyltransferase (MTASE) that methylates two adenine residues in complementary strands of bipartite DNA recognition sequence. Possible type I restriction/modification system specificity determinant HsdS (S protein) Rv2761c, (MTV002.26c), len: 364 aa. Possible hsdS, type I restriction/modification system specificity determinant (S protein), similar in part to other hsdS protein (S proteins) e.g. Q9P9X9|XF2741 from Xylella fastidiosa (412 aa), FASTA scores: opt: 252, E(): 7.4e-09, (24.95% identity in 401 aa overlap); N-terminus of Q9RC12 type I S-subunit from Lactobacillus delbrueckii (subsp. lactis) (389 aa), FASTA scores: opt: 232, E(): 1.4e-07, (28.1% identity in 185 aa overlap); N-terminus of P72419|sty|SBLI from Salmonella typhimurium (434 aa), FASTA scores: opt: 221, E(): 8e-07, (28.45% identity in 130 aa overlap); C-terminus of P17222|PRRB_ECOLI from Escherichia coli strain CTR5X (401 aa), FASTA scores: opt: 197, E(): 2.8e-05, (27.05% identity in 148 aa overlap); etc. Seems to belong to type-I restriction system S methylase family. I6YEB1 No information pathways O33303 I6YEB1 GO:0006304,GO:0003677 I6YEB1 NC_000962.3 Mycobrowser_v5 CDS 3074636 3075373 . + 0 Rv2765 Rv2765 Function unknown; probably involved in cellular metabolism. Probable alanine rich hydrolase Rv2765, (MTV002.30), len: 245 aa. Probable ala-rich hydrolase, similar to various hydrolases or hypothetical proteins e.g. Q9KYM6|SC9H11.13c putative hydrolase from Streptomyces coelicolor (251 aa), FASTA scores: opt: 630, E(): 1.4e-33, (43.1% identity in 246 aa overlap); Q9A5T9|CC2358 dienelactone hydrolase family protein from Caulobacter crescentus (286 aa), FASTA scores: opt: 592, E(): 4.5e-31, (38.45% identity in 242 aa overlap); Q9FCF1|2SCD46.33 putative hydrolase (dienelactone hydrolase family) from Streptomyces coelicolor (254 aa), FASTA scores: opt: 500, E(): 3.9e-25, (37.7% identity in 252 aa overlap); P73163|DLHH_SYNY3|SLL1298 putative carboxymethylenebutenolidase (dienelactone hydrolase) from Synechocystis sp. (strain PCC 6803) (246 aa), FASTA scores: opt: 276, E(): 1.3e-10, (26.95% identity in 230 aa overlap); etc. I6XF92 No intermediary metabolism and respiration O33307 I6XF92 GO:0008806 3.1.1.45 I6XF92 NC_000962.3 Mycobrowser_v5 CDS 3079309 3080457 . - 0 Rv2770c PPE44 Function unknown. May be involved in virulence. PPE family protein PPE44 Rv2770c, (MTV002.35c), len: 382 aa. PPE44, Member of the Mycobacterium tuberculosis PPE family, highly similar to many e.g. downstream ORF O33310|Rv2768c|MTV002.33c from M. tuberculosis (394 aa), FASTA scores: opt: 1135, E(): 2.2e-53, (62.15% identity in 391 aa overlap); and P96362|Rv1039c|MTCY10G2.10 from Mycobacterium tuberculosis (391 aa), FASTA scores: opt: 1010, E(): 1e-46, (55.95% identity in 395 aa overlap). Equivalent to AAK47159 from Mycobacterium tuberculosis strain CDC1551 (402 aa) but shorter 20 aa. Start changed since first submission (-20 aa). P9WHZ3 No PE/PPE Q79FA7 P9WHZ3 P9WHZ3 NC_000962.3 Mycobrowser_v5 CDS 3108416 3110065 . + 0 Rv2800 Rv2800 Function unknown; probably involved in cellular metabolism. Possible hydrolase Rv2800, (MTCY16B7.43c), len: 549 aa. Possible hydrolase, an esterase or an acylase. Similar, but longer in N-terminus, to esterases or acylases e.g. Q9L9D7|COCE cocaine esterase from Rhodococcus sp. MB1 'Bresler 1999' (574 aa), FASTA scores: opt: 510, E(): 3.1e-23, (33.6% identity in 571 aa overlap); Q9L3U2|STTE putative acylase from Streptomyces rochei (Streptomyces parvullus) (554 aa), FASTA scores: opt: 492, E(): 3.7e-22, (34.45% identity in 569 aa overlap); CAC49652|SMB21424 putative esterase or acylase protein from Rhizobium meliloti (Sinorhizobium meliloti) plasmid pSymB (578 aa), FASTA scores: opt: 405, E(): 7.1e-17, (34.45% identity in 569 aa overlap); etc. I6YEE6 No intermediary metabolism and respiration P71651 I6YEE6 GO:0008152,GO:0008239 3.-.-.- I6YEE6 NC_000962.3 Mycobrowser_v5 CDS 3081604 3082341 . - 0 Rv2773c dapB Involved in biosynthesis of diaminopimelate and lysine from aspartate semialdehyde (at the second step) [catalytic activity: 2,3,4,5-tetrahydrodipicolinate + NAD(P)(+) = 2,3-dihydrodipicolinate + NAD(P)H]. Dihydrodipicolinate reductase DapB (DHPR) Rv2773c, (MTV002.38c), len: 245 aa. DapB, dihydrodipicolinate reductase (see Pavelka et al., 1997), highly similar to many e.g. P40110|DAPB_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (248 aa), FASTA scores: opt: 1030, E(): 1.8e-58, (65.45% identity in 246 aa overlap); O86836|DAPB_STRCO|SC9A10.03 from Streptomyces coelicolor (250 aa), FASTA scores: opt: 997, E(): 2.3e-56, (61.15% identity in 247 aa overlap); P42976|DAPB_BACSU from Bacillus subtilis (267 aa), FASTA scores: opt: 608, E(): 1.7e-31, (45.95% identity in 209 aa overlap); P46829|DAPB_MYCBO from Mycobacterium bovis (see Cirillo et al., 1994) (271 aa), FASTA scores: opt: 505, E(): 6.3e-25, (36.2% identity in 246 aa overlap); etc. Belongs to the dihydrodipicolinate reductase family. P9WP23 No intermediary metabolism and respiration 1C3V,1P9L,1YL5,1YL6,1YL7 P72024 P9WP23 GO:0005737,GO:0019877,GO:0008839,GO:0055114 1.3.1.26 P9WP23 NC_000962.3 Mycobrowser_v5 CDS 3083374 3084303 . - 0 Rv2776c Rv2776c Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv2776c, (MTV002.41c), len: 309 aa. Probable oxidoreductase, similar to other oxidoreductases e.g. Q9KZ15|SC10B7.17 putative iron-sulfur oxidoreductase from Streptomyces coelicolor (364 aa), FASTA scores: opt: 846, E(): 1.2e-45, (46.75% identity in 308 aa overlap); O88034|SC5A7.28c iron-sulfur oxidoreductase beta subunit from Streptomyces coelicolor (313 aa), FASTA scores: opt: 745, E(): 2.3e-39, (41.45% identity in 316 aa overlap); P33164|PDR_BURCE|OPHA1 phthalate dioxygenase reductase from Burkholderia cepacia (Pseudomonas cepacia) (321 aa), FASTA scores: opt: 616, E(): 2.9e-31, (33.65% identity in 309 aa overlap); etc. Equivalent to AAK47165 from Mycobacterium tuberculosis strain CDC1551 (363 aa) but shorter 54 aa. Contains PS00197 2Fe-2S ferredoxins, iron-sulfur binding region signature and PS00063 Aldo/keto reductase family putative active site signature. Seems to belong to the 2FE2S plant-type ferredoxin family in the C-terminal section. O86347 No intermediary metabolism and respiration O86347 O86347 GO:0009055,GO:0046872,GO:0055114,GO:0016491,GO:0051537 1.-.-.- O86347 NC_000962.3 Mycobrowser_v5 CDS 3086820 3087935 . + 0 Rv2780 ald May play a role in cell wall synthesis as L-alanine is an important constituent of the peptidoglycan layer [catalytic activity: L-alanine + H(2)O + NAD(+) = pyruvate + NH(3) + NADH]. Secreted L-alanine dehydrogenase Ald (40 kDa antigen) (TB43) Rv2780, (MT2850, MTV002.45), len: 371 aa. Ald, secreted L-alanine dehydrogenase (40 kd antigen); equivalent to Q9CBV6|ALD|ML1532 L-alanine dehydrogenase from Mycobacterium leprae (371 aa), FASTA scores: opt: 2081, E(): 4e-115, (85.45% identity in 371 aa overlap). Also highly similar to others e.g. Q9S227|SCI51.13c from Streptomyces coelicolor (371 aa), FASTA scores: opt: 1575, E(): 2.3e-85, (66.05% identity in 371 aa overlap); Q9K827|BH3180 from Bacillus halodurans (371 aa), FASTA scores: opt: 1341, E(): 1.4e-71, (56.45% identity in 372 aa overlap); Q9RT70|DR1895 from Deinococcus radiodurans (390 aa), FASTA scores: opt: 1319, E(): 2.8e-70, (54.2% identity in 371 aa overlap); etc. Contains PS00836 and PS00837 Alanine dehydrogenase & pyridine nucleotide transhydrogenase signature 1 and 2. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WQB1 No intermediary metabolism and respiration 2VHV,2VHW,2VHX,2VHY,2VHZ,2VOE,2VOJ P30234 P9WQB1 GO:0005576,GO:0055114,GO:0000286 1.4.1.1 P9WQB1 NC_000962.3 Mycobrowser_v5 CDS 3089045 3090361 . - 0 Rv2782c pepR Function unknown; possibly hydrolyzes peptides and/or proteins Probable zinc protease PepR Rv2782c, (MTV002.47c), len: 438 aa. Probable pepR, protease/peptidase, equivalent to O32965|YR82_MYCLE|ML0855|MLCB22.26c hypothetical zinc protease from Mycobacterium leprae (445 aa), FASTA scores: opt: 2346, E(): 4.3e-146, (84.3% identity in 421 aa overlap). Also highly similar to others e.g. O86835|YA12_STRCO|SC9A10.02 from Streptomyces coelicolor (459 aa), FASTA scores: opt: 1394, E(): 1.1e-83, (51.9% identity in 416 aa overlap); Q04805|YMXG_BACSU|YMXG from Bacillus subtilis (409 aa), FASTA scores: opt: 1014, E(): 7.9e-59, (37.55% identity in 410 aa overlap); Q9KA85|BH2405 from Bacillus halodurans (413 aa), FASTA scores: opt: 967, E(): 9.6e-56, (38.6% identity in 417 aa overlap); etc. Contains PS00143 Insulinase family, zinc-binding region signature. Belongs to peptidase family M16, also known as the insulinase family. Cofactor: requires divalent cations for activity. Binds zinc. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P9WHT5 No intermediary metabolism and respiration P0A5S8 P9WHT5 GO:0006508,GO:0008270,GO:0004222 3.4.24.- P9WHT5 NC_000962.3 Mycobrowser_v5 CDS 3212162 3212956 . - 0 Rv2902c rnhB Degrades the ribonucleotide moiety on RNA-DNA hybrid molecules [catalytic activity: endonucleolytic cleavage to 5'- phosphomonoester]. Probable ribonuclease HII protein RnhB (RNase HII) Rv2902c, (MT2970, MTCY274.33c), len: 264 aa. Probable rnhB, ribonuclease HII, equivalent to O33022|RNH2_MYCLE|RNHB|ML1611|MLCB250.40 ribonuclease HII from Mycobacterium leprae (240 aa), FASTA scores: opt: 1242, E(): 6.9e-72, (76.75% identity in 245 aa overlap). Also similar (but longer ~20 aa) to others e.g. Q9HXY9|RNHB|PA3642 ribonuclease HII from Pseudomonas aeruginosa (201 aa), FASTA scores: opt: 572, E(): 3.1e-29, (52.7% identity in 184 aa overlap); Q9PEI7|RNH2_XYLFA|RNHB|XF1041 ribonuclease HII from Xylella fastidiosa (234 aa), FASTA scores: opt: 556, E(): 3.6e-28, (50.25% identity in 185 aa overlap); P10442|RNH2_ECOLI|RNHB|B0183 ribonuclease HII from Escherichia coli strain K-12 (213 aa), FASTA scores: opt: 519, E(): 7.4e-26, (48.65% identity in 183 aa overlap); etc. Belongs to the RNASE HII family. Cofactor: manganese (by similarity). P9WH01 No information pathways Q10793 P9WH01 GO:0005737,GO:0046872,GO:0004523,GO:0003723 3.1.26.4 P9WH01 NC_000962.3 Mycobrowser_v5 CDS 3093905 3094900 . - 0 Rv2786c ribF Involved in FAD biosynthesis [catalytic activity 1: ATP + riboflavin = ADP + FMN] [catalytic activity 2: ATP + FMN = diphosphate + FAD]. Probable bifunctional FAD synthetase/riboflavin biosynthesis protein RibF: riboflavin kinase (flavokinase) + FMN adenylyltransferase (FAD pyrophosphorylase) (FAD synthetase)(FAD diphosphorylase) (flavin adenine dinucleotide synthetase) Rv2786c, (MTV002.51c), len: 331 aa. Probable ribF, FAD synthetase/riboflavin biosynthesis protein, bifunctional enzyme, equivalent to O32968|RIBF|ML0852 riboflavin kinase from Mycobacterium leprae (331 aa), FASTA scores: opt: 1923, E(): 2.3e-115, (87.45% identity in 327 aa overlap). Also highly similar to many e.g. Q59263|RIBF_CORAM from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (338 aa), FASTA scores: opt: 899, E(): 5.7e-50, (45.8% identity in 321 aa overlap); Q9Z530|SC9F2.05c from Streptomyces coelicolor (318 aa), FASTA scores: opt: 862, E(): 1.3e-47, (52.45% identity in 324 aa overlap); P08391|RIBF_ECOLI|B0025|Z0029ECS0028 from Escherichia coli strains K12 and O157:H7 (313 aa), FASTA scores: opt: 517, E(): 1.3e-25, (36.05% identity in 305 aa overlap); etc. I6X5C9 No intermediary metabolism and respiration O33328 I6X5C9 GO:0009231,GO:0008531,GO:0003919 2.7.1.26 I6X5C9 NC_000962.3 Mycobrowser_v5 CDS 3098964 3100169 . - 0 Rv2790c ltp1 Possibly catalyzes the transfer of a great variety of lipids between membranes. Probable lipid-transfer protein Ltp1 Rv2790c, (MTV002.55c), len: 401 aa. Probable ltp1, lipid-transfer protein, highly similar to many eukaryotic sterol-carrier proteins/lipid-transfer protein precursors (see Ossendorp & Wirtz 1993) e.g. O62742|SCP2 sterol carrier protein X from Oryctolagus cuniculus (Rabbit) (547 aa), FASTA scores: opt: 1710, E(): 6e-102, (63.7% identity in 394 aa overlap); Q9QW19 3-oxoacyl-CoA thiolase homolog (fragment) from Rattus sp. (405 aa), FASTA scores: opt: 1696, E(): 3.8e-101, (63.2% identity in 394 aa overlap); P11915|NLTP_RAT|SCP2|SCP-2 nonspecific lipid-transfer protein precursor from Rattus norvegicus (Rat) (547 aa), FASTA scores: opt: 1696, E(): 4.8e-101, (63.2% identity in 394 aa overlap); P32020|NLTP_MOUSE|SCP2|SCP-2 nonspecific lipid-transfer protein precursor from Mus musculus (Mouse) (547 aa), FASTA scores: opt: 1681, E(): 4.3e-100, (62.7% identity in 394 aa overlap); etc. Contains PS00098 Thiolases acyl-enzyme intermediate signature and PS00737 Thiolases signature 2. Also similar to other M. tuberculosis proteins e.g. O06144|Rv1627c|MTCY01B2.19c (402 aa) (35.8% identity in 413 aa overlap). O33332 No cell wall and cell processes O33332 O33332 GO:0016747,GO:0008152 O33332 NC_000962.3 Mycobrowser_v5 CDS 3113658 3114812 . + 0 Rv2807 Rv2807 Function unknown Conserved hypothetical protein Rv2807, (MTCY16B7.36c), len: 384 aa. Conserved hypothetical protein, highly similar, but shorter 35 aa, to Q9KK74 hypothetical 47.4 KDA protein from Brevibacterium linens (418 aa), FASTA scores: opt: 1865, E(): 9.4e-116, (69.75% identity in 380 aa overlap); and with similarity with other hypothetical proteins or transposases e.g. Q981U5|MLR9230 protein from Rhizobium loti (Mesorhizobium loti) (504 aa), FASTA scores: opt: 636,, (36.05% identity in 377 aa overlap); CAC47689 putative transposase for insertion sequence ISRM18 from Rhizobium meliloti (Sinorhizobium meliloti) (507 aa), FASTA scores: opt: 553, E(): 6.6e-29, (33.5% identity in 370 aa overlap); etc. Also similar to Rv3128c|MTCY164.38c (336 aa) (47.2% identity in 339 aa overlap); and high similarity at N-terminal region with Rv2805|MTCY16B7.38c (79.2% identity in 101 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P71644 No conserved hypotheticals P71644 P71644 GO:0015074,GO:0003677 P71644 NC_000962.3 Mycobrowser_v5 CDS 3118224 3119036 . + 0 Rv2813 Rv2813 Function unknown Conserved hypothetical protein Rv2813, (MTCY16B7.30c), len: 270 aa. Conserved hypothetical protein, similar to various proteins (notably secreted proteins) e.g. Q9ZFL2 hypothetical 30.4 KDA protein from Bacillus stearothermophilus (266 aa), FASTA scores: opt: 518, E(): 1.4e-26, (33.85% identity in 266 aa overlap); P45754|GSPA_AERHY|EXEA general secretion pathway protein from Aeromonas hydrophila (547 aa), FASTA scores: opt: 386, E(): 1.1e-17, (32.05% identity in 265 aa overlap); Q9KPC7|VC2445 general secretion pathway protein A from Vibrio cholerae (529 aa), FASTA scores: opt: 366, E(): 2.2e-16, (31.1% identity in 270 aa overlap); Q56674|VC0403 mannose-sensitive hemagglutinin D from Vibrio cholerae (281 aa), FASTA scores: opt: 317, E(): 2.1e-13, (27.85% identity in 262 aa overlap); etc. Also highly similar to AAK40072 Rv2813-like protein from Mycobacterium celatum (270 aa), FASTA scores: opt: 1628, E(): 2.8e-99, (90.75% identity in 270 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6XFD1 No conserved hypotheticals P71638 I6XFD1 I6XFD1 NC_000962.3 Mycobrowser_v5 CDS 3120566 3121552 . - 0 Rv2814c Rv2814c Required for the transposition of the insertion element IS6110. Probable transposase Rv2814c, (MTCY16B7.29), len: 328 aa. Probable transposase subunit for IS6110. Identical to many other M. tuberculosis IS6110 transposase subunits. The transposase described here may be made by a frame shifting mechanism during translation that fuses Rv2814c and Rv2815c, the sequence UUUUAAAG (directly upstream of Rv2814c) maybe responsible for such a frameshifting event (see McAdam et al., 1990). Start changed since first submission (+ 16 aa). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WKH9 No insertion seqs and phages P0C5G8 P9WKH9 GO:0015074,GO:0006310,GO:0032196,GO:0003677 P9WKH9 NC_000962.3 Mycobrowser_v5 CDS 3397214 3398290 . - 0 Rv3037c Rv3037c Function unknown Conserved hypothetical protein Rv3037c, (MTV012.52c), len: 358 aa. Conserved hypothetical protein, similar in part to others e.g. O86799|SC6G4.36c from Streptomyces coelicolor (426 aa), FASTA scores: opt: 545, E(): 5.5e-27, (36.15% identity in 354 aa overlap); Q9UZW6|PAB0687 from Pyrococcus abyssi (386 aa), FASTA scores: opt: 262, E(): 3.5e-09, (31.0% identity in 200 aa overlap). P9WJZ3 No conserved hypotheticals P9WJZ3 P9WJZ3 NC_000962.3 Mycobrowser_v5 CDS 3128253 3128963 . - 0 Rv2821c Rv2821c Function unknown Conserved hypothetical protein Rv2821c, (MTCY16B7.21), len: 236 aa. Conserved hypothetical protein, similar to several hypothetical proteins e.g. Q9X2C9|TM1809 conserved hypothetical protein from Thermotoga maritima (247 aa), FASTA scores: opt: 318, E(): 8.2e-15, (39.45% identity in 213 aa overlap); O27152|MTH1080 conserved hypothetical protein from Methanothermobacter thermautotrophicus (245 aa), FASTA scores: opt: 294, E(): 3.9e-13, (34.8% identity in 224 aa overlap); BAB59251|TVG0114661 hypothetical protein from Thermoplasma volcanium (229 aa), FASTA scores: opt: 252, E(): 3.3e-10, (33.8% identity in 225 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WJF9 No conserved hypotheticals P71631 P9WJF9 P9WJF9 NC_000962.3 Mycobrowser_v5 CDS 3137271 3138020 . + 0 Rv2831 echA16 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Probable enoyl-CoA hydratase EchA16 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) Rv2831, (MTCY16B7.11c), len: 249 aa. Probable echA16, enoyl-CoA hydratase, similar to others e.g. O23468|AT4G16210 from Arabidopsis thaliana (Mouse-ear cress) (244 aa), FASTA scores: opt: 491, E(): 7.3e-25, (42.1% identity in 190 aa overlap); Q98LI4|MLL1009 from Rhizobium loti (Mesorhizobium loti) (258 aa), FASTA scores: opt: 491, E(): 7.6e-25, (40.75% identity in 248 aa overlap); O07137|ECH8_MYCLE|ML2402|MLCB1306.05c from Mycobacterium leprae (257 aa), FASTA scores: opt: 478, E(): 5.3e-24, (38.05% identity in 226 aa overlap); P76082|PAAF_ECOLI|B1393 from scherichia coli strain K12 (255 aa), FASTA scores: opt: 439, E(): 1.9e-21, (37.55% identity in 221 aa overlap); etc. Also similar to O53418|ECH8_MYCTU|ECHA8|Rv1070c|MT1100|MTV017.23c from Mycobacterium tuberculosis (257 aa), FASTA scores: opt: 471, E(): 1.5e-23, (38.05% identity in 226 aa overlap). I6YEH6 No lipid metabolism P71621 I6YEH6 GO:0016853,GO:0008152,GO:0004300 4.2.1.17 I6YEH6 NC_000962.3 Mycobrowser_v5 CDS 3138099 3139181 . - 0 Rv2832c ugpC Thought to be involved in active transport of Sn-glycerol-3-phosphate across the membrane (import). Responsible for energy coupling to the transport system. Probable Sn-glycerol-3-phosphate transport ATP-binding protein ABC transporter UgpC Rv2832c, (MTCY16B7.10), len: 360 aa. Probable ugpC, Sn-glycerol-3-phosphate transport ATP-binding protein ABC transporter (see Braibant et al., 2000), similar to others: CAC48805 probable glycerol-3-phosphate ABC transporter ATP-binding protein from Rhizobium meliloti (Sinorhizobium meliloti) plasmid pSymB (349 aa), FASTA scores: opt: 1018, E(): 4.1e-53, (48.6% identity in 356 aa overlap); Q98G42|MLL3499|UGPC SN-glycerol-3-phosphate transport ATP-binding protein from Rhizobium loti (Mesorhizobium loti) (366 aa), FASTA scores: opt: 1016, E(): 5.6e-53, (48.5% identity in 367 aa overlap). But also highly similar to many msiK proteins, ABC transporter ATP-binding proteins possibly involved in transport of cellolbiose and maltose (see Schlosser et al., 1997) e.g. P96483|MSIK MSIK protein from Streptomyces reticuli (377 aa), FASTA scores: opt: 1277, E(): 1.9e-68, (58.05% identity in 379 aa overlap); Q9L0Q1|MSIK ABC transporter ATP-binding protein from Streptomyces coelicolor (378 aa), FASTA scores: opt: 1276, E(): 2.1e-68, (57.65% identity in 380 aa overlap); Q54333|MSIK from Streptomyces lividans (314 aa), FASTA scores: opt: 1217, E(): 5.9e-65, (63.7% identity in 292 aa overlap); and other ABC-type sugar transport proteins. Also highly similar to O53482|Rv2038c|MTV018.25c ABC-type sugar transport protein from Mycobacterium tuberculosis (357 aa), FASTA scores: opt: 1248, E(): 9.4e-67, (56.8% identity in 354 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). I6X5H3 No cell wall and cell processes P71620 I6X5H3 GO:0043190,GO:0016887,GO:0016820,GO:0006810,GO:0005524 I6X5H3 NC_000962.3 Mycobrowser_v5 CDS 3140487 3141314 . - 0 Rv2834c ugpE Involved in active transport of Sn-glycerol-3-phosphate across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable Sn-glycerol-3-phosphate transport integral membrane protein ABC transporter UgpE Rv2834c, (MTCY16B7.08), len: 275 aa. Probable ugpE, Sn-glycerol-3-phosphate transport integral membrane protein ABC transporter (see citation below), similar to various permeases e.g. Q9KDY3|BH1078 glycerol-3-phosphate ABC transporter from Bacillus halodurans (270 aa), FASTA scores: opt: 620, E(): 4.3e-32, (34.7% identity in 268 aa overlap); Q9X0K6|TM1122 glycerol-3-phosphate ABC transporter permease protein from Thermotoga maritima (276 aa), FASTA scores: opt: 605, E(): 3.9e-31, (32.5% identity in 274 aa overlap); AAG58557|UGPE SN-glycerol 3-phosphate transport system (integral membrane protein) from Escherichia coli strain O157:H7 and EDL933 (281 aa), FASTA scores: opt: 574, E(): 3.7e-29, (32.95% identity in 264 aa overlap); P10906|UGPE_ECOLI|B3451 SN-glycerol-3-phosphate transport system permease protein from Escherichia coli strain K12 (281 aa), FASTA scores: opt: 569, E(): 7.6e-29, (32.6% identity in 264 aa overlap); etc. Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature. I6Y1U3 No cell wall and cell processes P71618 I6Y1U3 GO:0005886,GO:0006810,GO:0005215,GO:0016021 I6Y1U3 NC_000962.3 Mycobrowser_v5 CDS 3141311 3142222 . - 0 Rv2835c ugpA Involved in active transport of Sn-glycerol-3-phosphate across the membrane (import). Responsible for the translocation of the substrate across the membrane. Probable Sn-glycerol-3-phosphate transport integral membrane protein ABC transporter UgpA Rv2835c, (MTCY1B7.07), len: 303 aa. Probable ugpA, Sn-glycerol-3-phosphate transport integral membrane protein ABC transporter (see citation below), similar to various permeases e.g. Q9RK71|SCF11.19 probable sugar transporter inner membrane protein from Streptomyces coelicolor (316 aa), FASTA scores: opt: 643, E(): 3.1e-35, (38.85% identity in 291 aa overlap); Q9KDY4|BH1077 glycerol-3-phosphate ABC transporter (permease) from Bacillus halodurans (315 aa), FASTA scores: opt: 548, E(): 6.2e-29, (31.5% identity in 295 aa overlap); AAK78407|CAC0427 glycerol-3-phosphate ABC-transporter, permease component from Clostridium acetobutylicum (304 aa), FASTA scores: opt: 538, E(): 2.8e-28, (29.1% identity in 292 aa overlap); etc. Contains PS00062 Aldo/keto reductase family signature 2, and PS00402 Binding-protein-dependent transport systems inner membrane comp signature. I6XFF3 No cell wall and cell processes P71617 I6XFF3 GO:0005886,GO:0006810,GO:0005215,GO:0016021 I6XFF3 NC_000962.3 Mycobrowser_v5 CDS 3728624 3729355 . + 0 Rv3342 Rv3342 Causes methylation Possible methyltransferase (methylase) Rv3342, (MTV016.42), len: 243 aa. Possible methyltransferase, similar to various proteins e.g. Q9I5X8|PA0558 hypothetical protein from Pseudomonas aeruginosa (255 aa), FASTA scores: opt: 496, E(): 4.4e-24, (39.85% identity in 236 aa overlap); Q9XBC9|CZA382.22c putative rRNA methylase from Amycolatopsis orientalis (259 aa), FASTA scores: opt: 473, E(): 1.2e-22, (42.45% identity in 245 aa overlap); Q9UTA8|SPAC25B8.10 putative methyltransferase from Schizosaccharomyces pombe (Fission yeast) (256 aa), FASTA scores: opt: 470, E(): 1.9e-22, (35.7% identity in 238 aa overlap); and Q9UTA9|SPAC25B8.09 putative methyltransferase from Schizosaccharomyces pombe (Fission yeast) (251 aa), FASTA scores: opt: 418, E(): 3.4e-19, (31.2% identity in 237 aa overlap); etc. Start uncertain. Belongs to the methyltransferase superfamily. P9WK01 No intermediary metabolism and respiration P65348 P9WK01 GO:0008168,GO:0008152 2.1.1.- P9WK01 NC_000962.3 Mycobrowser_v5 CDS 3148385 3149428 . - 0 Rv2841c nusA Could participates in both the termination and antitermination of transcription. Probable N utilization substance protein A NusA Rv2841c, (MTCY24A1.16, MTCY16B7.01), len: 347 aa. Probable nusA, N-utilization substance protein A, equivalent to Q9Z5J1|NUSA|ML1558 probable transcription termination/antitermination factor from Mycobacterium leprae (347 aa), FASTA scores: opt: 2054, E(): 5.4e-120, (91.95% identity in 347 aa overlap). Also highly similar to others e.g. Q9KYR1|SC5H4.28 putative transcriptional termination/antitermination factor from Streptomyces coelicolor (340 aa), FASTA scores: opt: 1346, E(): 4.3e-76, (63.35% identity in 341 aa overlap); P32727|NUSA_BACSU N utilization substance protein A (371 aa), FASTA scores: opt: 847, E(): 4.1e-45, (43.95% identity in 346 aa overlap); Q9KA74|NUSA|BH2416 transcriptional terminator from Bacillus halodurans (382 aa), FASTA scores: opt: 846, E(): 4.8e-45, (43.15% identity in 373 aa overlap); etc. Belongs to the NUSA family. P9WIV3 No information pathways 1K0R,2ASB,2ATW P0A5M2 P9WIV3 GO:0005515,GO:0031564,GO:0003700,GO:0006353,GO:0003723 P9WIV3 NC_000962.3 Mycobrowser_v5 CDS 3151202 3152950 . - 0 Rv2845c proS Involved in translation mechanism [catalytic activity: ATP + L-proline + tRNA(pro) = AMP + pyrophosphate + L-prolyl-tRNA(pro)]. Probable prolyl-tRNA synthetase ProS (proline--tRNA ligase) (PRORS) (global RNA synthesis factor) (proline translase) Rv2845c, (MTCY24A1.12), len: 582 aa. Probable proS, prolyl-tRNA synthetase, highly similar to others e.g. Q9KYR6|SYP_STRCO|pros|SC5H4.23 from Streptomyces coelicolor (567 aa), FASTA scores: opt: 1161, E(): 9e-64, (57.15% identity in 574 aa overlap); P56124|SYP_HELPY|pros|HP0238 from Helicobacter pylori (Campylobacter pylori) (577 aa), FASTA scores: opt: 1082, E(): 6.6e-59, (37.8% identity in 553 aa overlap); P16659|SYP_ECOLI|pros|DRPA|B0194 from Escherichia coli strain K12 (572 aa), FASTA scores: opt: 926, E(): 2.6e-49, (39.85% identity in 587 aa overlap); etc. Contains PS00179 Aminoacyl-transfer RNA synthetases class-II signature 1. Belongs to class-II aminoacyl-tRNA synthetase family. P9WFT9 No information pathways O05814 P9WFT9 GO:0005737,GO:0004827,GO:0006433,GO:0005524 6.1.1.15 P9WFT9 NC_000962.3 Mycobrowser_v5 CDS 3156148 3157521 . - 0 Rv2848c cobB Involved in the cobalamin biosynthesis. Responsible for the amidation of carboxylic groups at position a and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation. Probable cobyrinic acid A,C-diamide synthase CobB Rv2848c, (MTCY24A1.09), len: 457 aa. Probable cobB, cobyrinic acid A,C-diamide synthase, highly similar to others e.g. O27509|COBB_METTH|MTH1460 from Methanobacterium thermoautotrophicum (447 aa), FASTA scores: opt: 980, E(): 1.3e-49, (39.65% identity in 454 aa overlap); Q9KBM8|BH1898 from Bacillus halodurans (465 aa), FASTA scores: opt: 928, E(): 1.4e-46, (37.0% identity in 457 aa overlap); O68108|COBB_RHOCA from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (435 aa), FASTA scores: opt: 921, E(): 3.3e-46, (39.35% identity in 437 aa overlap); etc. Belongs to the COBB/COBQ family, COBB subfamily. P9WP97 No intermediary metabolism and respiration P63835 P9WP97 GO:0015420,GO:0042242,GO:0006541,GO:0009236 P9WP97 NC_000962.3 Mycobrowser_v5 CDS 3398425 3399408 . - 0 Rv3038c Rv3038c Function unknown Conserved protein Rv3038c, (MTV012.53c), len: 327 aa. Conserved protein, equivalent to Q9CBQ9|ML1723 hypothetical protein from Mycobacterium leprae (327 aa), FASTA scores: opt: 1843, E(): 6.1e-108, (80.75% identity in 327 aa overlap). Weak similarity with e.g. Q9KZI3|SCG8A.16 putative methyltransferase from Streptomyces coelicolor (199 aa), FASTA scores: opt: 227, E(): 3.9e-07, (31.95% identity in 191 aa overlap) and O52570 methyltransferase from Amycolatopsis mediterranei (272 aa), FASTA scores: opt: 228, E(): 4.3e-07, (31.7% identity in 164 aa overlap). Contains PS00044 Bacterial regulatory proteins, lysR family signature but shows no similarity to known LysR family members. I6YAZ1 No conserved hypotheticals O53285 I6YAZ1 GO:0008168,GO:0008152 I6YAZ1 NC_000962.3 Mycobrowser_v5 CDS 3213912 3214253 . - 0 Rv2904c rplS This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site. 50S ribosomal protein L19 RplS Rv2904c, (MTCY274.35c), len: 113 aa. rplS, 50S ribosomal protein L19, equivalent to O33020|RL19_MYCLE 50S ribosomal protein L19 from Mycobacterium leprae (113 aa), FASTA scores: opt: 702, E(): 1.4e-45, (93.8% identity in 113 aa overlap). Also highly similar to others e.g. O69883|RL19_STRCO from Streptomyces coelicolor (116 aa), FASTA scores: opt: 571, E(): 9.5e-36, (77.25% identity in 110 aa overlap); O31742|RL19_BACSU from Bacillus subtilis (115 aa), FASTA scores: opt: 523, E(): 3.8e-32, (72.9% identity in 107 aa overlap); RL19_BACST|P30529 from Bacillus stearothermophilus (116 aa), FASTA scores: opt: 518, E(): 9.1e-32, (71.7% identity in 106 aa overlap); etc. Belongs to the L19P family of ribosomal proteins. P9WHC9 No information pathways P66080 P9WHC9 GO:0003735,GO:0006412,GO:0005840 P9WHC9 NC_000962.3 Mycobrowser_v5 CDS 3160580 3162061 . - 0 Rv2852c mqo Involved in tricarboxylic acid cycle [catalytic activity: (S)-malate + acceptor = oxaloacetate + reduced acceptor]. Probable malate:quinone oxidoreductase Mqo (malate dehydrogenase [acceptor]) Rv2852c, (MT2918, MTCY24A1.05), len: 493 aa. Probable mqo, malate:quinone oxidoreductase, highly similar to others e.g. O69282|MQO_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (499 aa), FASTA scores: opt: 1701, E(): 1.2e-101, (50.7% identity in 495 aa overlap); Q9Z9Q7|BH3960 from Bacillus halodurans (500 aa), FASTA scores: opt: 1632, E(): 3.3e-97, (48.55% identity in 486 aa overlap); Q9HYF4|MQOA|PA3452 from Pseudomonas aeruginosa (523 aa), FASTA scores: opt: 1604, E(): 2.1e-95, (49.1% identity in 487 aa overlap) (N-terminus longer); P33940|MQO_ECOLI|B2210 from Escherichia coli strain K12 (548 aa), FASTA scores: opt: 1525, E(): 2.7e-90, (48.15% identity in 492 aa overlap); etc. Belongs to the MQO family. Cofactors: FAD. P9WJP5 No intermediary metabolism and respiration P65419 P9WJP5 GO:0055114,GO:0006099,GO:0008924 1.1.5.4 NC_000962.3 Mycobrowser_v5 CDS 3165205 3166584 . + 0 Rv2855 mtr Involved in reduction of mycothiol. NADPH-dependent mycothiol reductase Mtr Rv2855, (MTCY24A1.02c), len: 459 aa. Mtr, NADPH-dependent mycothiol reductase, proven enzymatically but previously described as glutathione reductase homolog (gene name: gorA) (see citation below). Similar to others e.g. Q9L7K8|MERA mercuric reductase from Streptomyces sp. CHR28 (474 aa), FASTA scores: opt: 719, E(): 9e-38, (35.2% identity in 460 aa overlap); P30341|MERA_STRLI mercuric reductase from Streptomyces lividans (474 aa), FASTA scores: opt: 712, E(): 2.5e-37, (34.95% identity in 455 aa overlap); Q98ED5|MLL4296 ferric leghemoglobin reductase-2 precursor, dihydrolipoamide dehydrogenase from Rhizobium loti (Mesorhizobium loti) (468 aa), FASTA scores: opt: 670, E(): 1.1e-34, (30.8% identity in 471 aa overlap); etc. Belongs to the pyridine nucleotide-disulphide oxidoreductases class-I. Cofactor: FAD. P9WHH3 No intermediary metabolism and respiration O07927 P9WHH3 GO:0045454,GO:0005737,GO:0055114,GO:0016491,GO:0050660 1.-.-.- P9WHH3 NC_000962.3 Mycobrowser_v5 CDS 3166684 3167802 . + 0 Rv2856 nicT Seems involved in nickel incorporation. Thought to be involved in transport of nickel across the membrane. Responsible for the translocation of the substrate across the membrane. Possible nickel-transport integral membrane protein NicT Rv2856, (MTCY24A1.01c), len: 372 aa. Possible nicT, nickel-transport integral membrane protein, similar to transport proteins and hydrogenase cluster proteins e.g. BAB58860|SAV2698 hypothetical 37.9 KDA protein from Staphylococcus aureus subsp. aureus Mu50 (338 aa), FASTA scores: opt: 1082, E(): 7.1e-60, (48.05% identity in 335 aa overlap); Q97ZB2|HOXN high-affinity nickel-transport protein from Sulfolobus solfataricus (373 aa), FASTA scores: opt: 922, E(): 6.6e-50, (42.2% identity in 372 aa overlap); P23516|HOXN_ALCEU high-affinity nickel transport protein (integral membrane protein) from Alcaligenes eutrophus (Ralstonia eutropha) (351 aa), FASTA scores: opt: 904, E(): 8.3e-49, (41.9% identity in 339 aa overlap); Q45247|HUPN_BRAJA hydrogenase nickel incorporation protein from Bradyrhizobium japonicum (381 aa), FASTA scores: opt: 853, E(): 1.3e-45, (41.65% identity in 329 aa overlap); etc. Seems to belong to the HOXN/HUPN/NIXA family of nickel transporters (NiCoT family). I6YEJ7 No cell wall and cell processes O05804 I6YEJ7 GO:0016021,GO:0046872,GO:0015099,GO:0015675,GO:0055085 NC_000962.3 Mycobrowser_v5 CDS 3169356 3170723 . - 0 Rv2858c aldC Oxidizes a wide variety of aldehydes [catalytic activity: aldehyde + NAD(+) + H(2)O = acid + NADH]. Probable aldehyde dehydrogenase AldC Rv2858c, (MTV003.04c), len: 455 aa. Probable aldC, aldehyde dehydrogenase, similar to many e.g. O88069|SCI35.34c putative aldehyde dehydrogenase from Streptomyces coelicolor (483 aa), FASTA scores: opt: 1872, E(): 6.4e-109, (64.5% identity in 448 aa overlap); Q9FAB1|ALDH|BT-ALDH aldehyde dehydrogenase from Bacillus thermoleovorans (497 aa), FASTA scores: opt: 1157, E(): 2.1e-64, (44.3% identity in 458 aa overlap); O33455|CYMC P-CUMIC aldehyde dehydrogenase from Pseudomonas putida (494 aa), FASTA scores: opt: 1149, E(): 6.5e-64, (43.15% identity in 452 aa overlap); P40047|DHA5_YEAST|ALD5|ALDH5|ALD3|YER073W aldehyde dehydrogenase from Saccharomyces cerevisiae (Baker's yeast) (519 aa), FASTA scores: opt: 1091, E(): 2.7e-60, (38.55% identity in 459 aa overlap); P80668|FEAB_ECOLI|PADA|MAOB|B1385 phenylacetaldehyde dehydrogenase from Escherichia coli strain K12 (499 aa), FASTA scores: opt: 1074, E(): 3e-59, (42.2% identity in 462 aa overlap); etc. Also similar to many M. tuberculosis dehydrogenases e.g. P71823|Rv0768|MTCY369.13 (489 aa), FASTA score: (38.1% identity in 467 aa overlap). Contains PS00687 Aldehyde dehydrogenases glutamic acid active site and PS00070 Aldehyde dehydrogenases cysteine active site. Belongs to the aldehyde dehydrogenases family. O33340 No intermediary metabolism and respiration O33340 O33340 GO:0055114,GO:0004029 1.2.1.3 O33340 NC_000962.3 Mycobrowser_v5 CDS 3170720 3171646 . - 0 Rv2859c Rv2859c Function unknown; probably involved in cellular metabolism. Possible amidotransferase Rv2859c, (MTV003.05c), len: 308 aa. Possible amidotransferase, equivalent (but longer 58 aa) to Q9CBU9|ML1573 possible amidotransferase from Mycobacterium leprae (249 aa), FASTA scores: opt: 1226, E(): 3e-64, (71.55% identity in 239 aa overlap). Also similar to other amidotransferases and hypothetical proteins, but shorter in N-terminus e.g. O88072|SCI35.37 hypothetical 25.3 KDA protein from Streptomyces coelicolor (242 aa), FASTA scores: opt: 683, E(): 1.2e-32, (47.65% identity in 235 aa overlap); AAK79730|Q97I88|CAC1764 predicted glutamine amidotransferase from Clostridium acetobutylicum (241 aa), FASTA scores: opt: 458, E(): 1.6e-19, (32.95% identity in 246 aa overlap); AAK75201|Q97QV9|SP1089 glutamine amidotransferase class I from Streptococcus pneumoniae (229 aa), FASTA scores: opt: 431, E(): 5.6e-18, (34.75% identity in 236 aa overlap); etc. Contains three 17 aa repeats at the N-terminus very similar to those in other Mycobacterium tuberculosis proteins e.g. Q10699|YY30_MYCTU|Rv2090|MT2151|MTCY49.30 putative 5'-3' exonuclease RV2090. O33341 No intermediary metabolism and respiration O33341 O33341 GO:0016787,GO:0016874,GO:0016740,GO:0006541 6.3.5.-,2.-.-.- O33341 NC_000962.3 Mycobrowser_v5 CDS 3175454 3177265 . - 0 Rv2864c Rv2864c Function unknown, possibly involved in cell wall biosynthesis. Possible penicillin-binding lipoprotein Rv2864c, (MTV003.10c), len: 603 aa. Possible penicillin-binding lipoprotein, probably located in periplasm, equivalent to Q9CBU6|ML1577 probable penicillin binding protein from Mycobacterium leprae (608 aa), FASTA scores: opt: 3352, E(): 2.1e-193, (81.5% identity in 606 aa overlap). Also shows some similarity to others e.g. P72405|PCBR from Streptomyces clavuligerus (551 aa), FASTA scores: opt: 543, E(): 6.1e-25, (28.4% identity in 567 aa overlap); Q9F2L0|SCH63.18c from Streptomyces coelicolor (546 aa), FASTA scores: opt: 519, E(): 1.7e-23, (29.3% identity in 577 aa overlap); Q9RKD1|SCE87.07 from Streptomyces coelicolor (541 aa), FASTA scores: opt: 472, E(): 1.1e-20, (34.3% identity in 318 aa overlap); etc. Equivalent to AAK47258 from Mycobacterium tuberculosis strain CDC1551 (618 aa) but shorter 15 aa. Contains signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site, and PS00017 ATP/GTP-binding site motif A (P-loop). O33346 No cell wall and cell processes O33346 O33346 GO:0009273,GO:0046677,GO:0008658 O33346 NC_000962.3 Mycobrowser_v5 CDS 3180548 3181762 . - 0 Rv2869c rip Controls membrane composition Membrane bound metalloprotease Rv2869c, (MTV003.15c), len: 404 aa. Rip, metalloprotease, regulates intramembrane proteolysis and controls membrane composition (rip, see Makinoshima and Glickman, 2005). Similar to site two protease (S2P) in higher eukaryotes. Conserved transmembrane protein, equivalent to Q9CBU4|ML1582 probable integral membrane protein from Mycobacterium leprae (404 aa), FASTA scores: opt: 2250, E(): 1.1e-128, (82.2% identity in 404 aa overlap). Also weakly similar to other membrane proteins or hypothetical proteins e.g. Q9A710|CC1916 putative membrane-associated zinc metalloprotease from Caulobacter crescentus (398 aa), FASTA scores: opt: 368, E(): 7.8e-15, (28.1% identity in 427 aa overlap). Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). Cleaves PbpB|Rv2163c in a Zn2+ -dependent manner (See Mukherjee et al., 2009). Cleaves proteins RskA|Rv0444c, RslA|Rv0736, and Rv3912, in M. tuberculosis Erdman (See Sklar et al., 2010). P9WHS3 No cell wall and cell processes O33351 P9WHS3 GO:0046872,GO:0004222,GO:0005886,GO:0005515,GO:0006508,GO:0016021 3.4.24.- P9WHS3 NC_000962.3 Mycobrowser_v5 CDS 3184847 3186934 . + 0 Rv2874 dipZ Could be involved in cytochrome-C biogenesis. Possible integral membrane C-type cytochrome biogenesis protein DipZ Rv2874, (MT2942, MTCY274.05), len: 695 aa. Possible dipZ, cytochrome c-type biogenesis protein (see citation below), probable integral membrane protein, similar in part to others or hypothetical proteins e.g. CAC48606|SMB20213 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (627 aa), FASTA scores: opt: 844, E(): 7.3e-43, (32.65% identity in 643 aa overlap); Q9ZMH0|CCDA or JHP0250 putative cytochrome C-type biogenesis protein from Helicobacter pylori J99 (Campylobacter pylori J99) (239 aa), FASTA scores: opt: 250, E(): 1.4e-07, (27.3% identity in 227 aa overlap); Q9LA04|CCDA C-type cytochrome biogenesis protein from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (252 aa), FASTA scores: opt: 245, E(): 2.9e-07, (27.85% identity in 244 aa overlap); etc. Also similar to O06393|CCSA|Rv0527|MTCY25D10.06 cytochrome C-type biogenesis protein from Mycobacterium tuberculosis (259 aa), FASTA scores: opt: 280, E(): 2.4e-09, (29.3% identity in 239 aa overlap). P9WG63 No intermediary metabolism and respiration 2HYX Q10801 P9WG63 GO:0045454,GO:0017004,GO:0016021,GO:0055114,GO:0016491,GO:0005886,GO:0016209 P9WG63 NC_000962.3 Mycobrowser_v5 CDS 3188008 3188871 . - 0 Rv2877c Rv2877c Unknown; possibly involved in transport of mercury across the membrane. Probable conserved integral membrane protein Rv2877c, (MTCY274.08c), len: 287 aa. Probable conserved integral membrane protein, Mer family possibly involved in transport of mercury, similar to others, and to the fourth protein of the mercury resistance operon of Streptomyces sp (or other organisms), and to putative cytochrome-c biogenesis proteins e.g. Q9XBD1|CZA382.20C putative integral membrane transporter from Amycolatopsis orientalis (298 aa), FASTA scores: opt: 913, E(): 7.6e-46, (51.55% identity in 293 aa overlap); P30344|MER4_STRLI mercury resistance probable HG transport protein from Streptomyces lividans (319 aa), FASTA scores: opt: 427, E(): 1.2e-17, (32.85% identity in 289 aa overlap); Q9M5P3 putative cytochrome C biogenesis protein precursor from Arabidopsis thaliana (Mouse-ear cress) (354 aa), FASTA scores: opt: 229, E(): 4e-06, (29.85% identity in 221 aa overlap); etc. Contains PS00044 Bacterial regulatory proteins, lysR family signature. Note that previously known as merT. I6YEL8 No cell wall and cell processes Q7D6F5 I6YEL8 GO:0016020,GO:0055114,GO:0017004 NC_000962.3 Mycobrowser_v5 CDS 3383885 3385066 . - 0 Rv3025c iscS Catalyzes the removal of elemental sulfur from cysteine to produce alanine. Cysteine desulfurase IscS (NIFS protein homolog) (nitrogenase metalloclusters biosynthesis protein NIFS) Rv3025c, (MTV012.40c), len: 393 aa. IscS (alternate gene name: nifS), cysteine desulfurase (NifS-like protein) , equivalent to MLCB637.06|O33098 NIFS-like protein from Mycobacterium leprae (396 aa), FASTA scores: opt: 2186, E(): 2.7e-122, (84.9% identity in 391 aa overlap). Also highly similar to many e.g. O86581|SC2A11.20 putative pyridoxal-phosphate-dependent aminotransferase from Streptomyces coelicolor (389 aa), FASTA scores: opt: 1568, E(): 1.1e-85, (61.7% identity in 389 aa overlap); P57795|ISCS|NIFS cysteine desulfurase (NIFS protein homolog) from Methanosarcina thermophila (404 aa), FASTA scores: opt: 1059, E(): 1.6e-55, (46.2% identity in 381 aa overlap); O54055|ISCS_RUMFL|ISCS|NIFS cysteine desulfurase from Ruminococcus flavefaciens (396 aa), FASTA scores: opt: 973, E(): 2e-50, (43.3% identity in 381 aa overlap); P57794|NIFS_ACEDI cysteine desulfurase from Acetobacter diazotrophicus (400 aa), FASTA scores: opt: 958, E(): 1.6e-49, (41.1% identity in 392 aa overlap); etc. Also similar to Rv1464|MTV007.11 from Mycobacterium tuberculosis. Contains PS00595 Aminotransferases class-V pyridoxal-phosphate attachment site. Belongs to class-V of pyridoxal-phosphate-dependent aminotransferases, NIFS/ISCS subfamily. Cofactor: pyridoxal phosphate (by similarity). P9WQ71 No intermediary metabolism and respiration O53272 P9WQ71 GO:0008152,GO:0030170,GO:0008483,GO:0016829 4.4.1.- P9WQ71 NC_000962.3 Mycobrowser_v5 CDS 3189583 3190152 . - 0 Rv2879c Rv2879c Function unknown Conserved hypothetical protein Rv2879c, (MTCY274.10c), len: 189 aa. Conserved hypothetical protein, similar to others e.g. C-terminus of Q9RVT6|DR0936 conserved hypothetical protein from Deinococcus radiodurans (346 aa), FASTA scores: opt: 505, E(): 1e-26, (46.5% identity in 185 aa overlap); O34617|YLON_BACSU hypothetical 41.6 KDA protein from Bacillus subtilis (363 aa), FASTA scores: opt: 459, E(): 1.2e-24, (40.5% identity in 185 aa overlap); YFGB_ECOLI|P36979 hypothetical 43.1 kDa protein from Escherichia coli (384 aa), FASTA scores, opt: 410, E(): 2.8e-21, (41.7% identity in 187 aa overlap); etc. Appears to be a frame shift with respect to following ORF but we can detect no error in the cosmid sequence to account for this. P0A644 No conserved hypotheticals P0A644 P0A644 GO:0008173,GO:0008757,GO:0005737,GO:0046872,GO:0006364,GO:0051539 2.1.1.- P9WH15 NC_000962.3 Mycobrowser_v5 CDS 3190701 3191621 . - 0 Rv2881c cdsA Involved in the phospholipid biosynthesis [catalytic activity: CTP + phosphatidate = pyrophosphate + CDP-diacylglycerol]. Probable integral membrane phosphatidate cytidylyltransferase CdsA (CDP-diglyceride synthetase) (CDP-diglyceride pyrophosphorylase) (CDP-diacylglycerol synthase) (CDS) (CTP:phosphatidate cytidylyltransferase) (CDP-DAG synthase) (CDP-DG synthetase) Rv2881c, (MTCY274.12c), len: 306 aa. Probable cdsA, phosphatidate cytidylyltransferase, integral membrane protein, equivalent to Q9CBU1|CDSA_MYCLE|ML1589 phosphatidate cytidylyltransferase from Mycobacterium leprae (312 aa), FASTA scores: opt: 1470, E(): 1.1e-84, (70.3% identity in 313 aa overlap). Also similar to others e.g. Q9KPV7|VC2255 from Vibrio cholerae (280 aa), FASTA scores: opt: 383, E(): 1.1e-16, (29.3% identity in 280 aa overlap); Q9CDT2|CDSA from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (267 aa), FASTA scores: opt: 361, E(): 2.6e-15, (29.05% identity in 265 aa overlap); P06466|CDSA_ECOLI|CDS|B0175|Z0186|ECS0177 from Escherichia coli strains K12 and O157:H7 (249 aa), FASTA scores: opt: 352, E(): 9.2e-15, (40.4% identity in 156 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the CDS family. P9WPF7 No lipid metabolism P63758 P9WPF7 GO:0004605,GO:0008654,GO:0005886,GO:0016021 2.7.7.41 P9WPF7 NC_000962.3 Mycobrowser_v5 CDS 3193393 3194151 . + 0 Rv2884 Rv2884 Involved in transcriptional mechanism Probable transcriptional regulatory protein Rv2884, (MTCY274.15), len: 252 aa. Probable transcriptional regulatory protein, highly similar to others e.g. Q05943|GLNR_STRCO|SCD84.26c transcriptional regulatory protein from Streptomyces coelicolor (267 aa), FASTA scores: opt: 609, E(): 2.7e-34, (46.4% identity in 224 aa overlap); Q55733|SLL0396 regulatory components of sensory transduction system from Synechocystis sp. strain PCC 6803 (224 aa), FASTA scores: opt: 330, E(): 3e-15, (31.8% identity in 217 aa overlap); Q9A4S3|CC2757 DNA-binding response regulator from Caulobacter crescentus (223 aa), FASTA scores: opt: 311, E(): 6e-14, (30.3% identity in 221 aa overlap); etc. Also highly similar to O53830|Rv0818|MTV043.10 putative regulatory protein from Mycobacterium tuberculosis (255 aa), FASTA scores: opt: 665, E(): 3.8e-38, (47.6% identity in 227 aa overlap). The N-terminal region is similar to that of other regulatory components of sensory transduction systems. I6X5M3 No regulatory proteins Q10808 I6X5M3 GO:0005622,GO:0006355,GO:0000156,GO:0000160,GO:0003677 I6X5M3 NC_000962.3 Mycobrowser_v5 CDS 3198292 3199107 . - 0 Rv2889c tsf Associates with the EF-TU.GDP complex and induces the exchange of GDP to GTP, it remains bound to the aminoacyl-tRNA. EF-TU.GTP complex up to the GTP hydrolysis stage on the ribosome. Probable elongation factor Tsf (EF-ts) Rv2889c, (MTCY274.20c), len: 271 aa. Probable tsf, elongation factor, equivalent to O33039|EFTS_MYCLE|TSF|ML1597|MLCB250.64 elongation factor from Mycobacterium leprae (276 aa), FASTA scores: opt: 1430, E(): 1.9e-80, (83.7% identity in 276 aa overlap). Also highly similar to others e.g. Q9X5Z9|EFTS_STRRA|TSF from Streptomyces ramocissimus (278 aa), FASTA scores: opt: 928, E(): 1.1e-49, (57.05% identity in 277 aa overlap); O31213|EFTS_STRCO|TSF|SC2E1.42 from Streptomyces coelicolor (278 aa), FASTA scores: opt: 927, E(): 1.3e-49, (56.3% identity in 277 aa overlap); P80700|EFTS_BACSU|TSF from Bacillus subtilis (292 aa), FASTA scores: opt: 650, E(): 1.3e-32, (43.85% identity in 276 aa overlap); etc. Contains PS01127 Elongation factor Ts signature 2. Belongs to the EF-ts family. P9WNM1 No information pathways Q10788 P9WNM1 GO:0003746,GO:0006414,GO:0005737 P9WNM1 NC_000962.3 Mycobrowser_v5 CDS 3202420 3203397 . + 0 Rv2893 Rv2893 Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv2893, (MTCY274.24), len: 325 aa. Possible oxidoreductase, showing similarity with various proteins and/or oxidoreductases e.g. Q9AE05|RIF11 eleventh protein in the rif biosynthetic gene cluster from Amycolatopsis mediterranei (Nocardia mediterranei) (294 aa), FASTA scores: opt: 270, E(): 4.8e-10, (34.5% identity in 313 aa overlap); O52567 reductase from Amycolatopsis mediterranei (Nocardia mediterranei) (153 aa), FASTA scores: opt: 251, E(): 5e-09, (42.4% identity in 125 aa overlap); Q58929|mer|MJ1534 F420-dependent methylenetetrahydromethanopterin reductase from Methanococcus jannaschii (331 aa), FASTA scores: opt: 249, E(): 1.2e-08, (29.7% identity in 283 aa overlap); etc. Also some similarity with others proteins from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. P71844|Rv0791c|MTCY369.35c putative oxidoreductase (347 aa), FASTA scores: opt: 264, E(): 1.3e-09, (29.05% identity in 272 aa overlap); and P96809|Rv0132|MTCI5.06c putative oxidoreductase (360 aa), FASTA scores: opt: 260, E(): 2.4e-09, (33.05% identity in 239 aa overlap). I6YEN3 No intermediary metabolism and respiration Q10814 I6YEN3 GO:0016491,GO:0055114 1.-.-.- I6YEN3 NC_000962.3 Mycobrowser_v5 CDS 3206431 3207942 . - 0 Rv2897c Rv2897c Function unknown Conserved hypothetical protein Rv2897c, (MTCY274.28c), len: 503 aa. Conserved hypothetical protein, possibly Mg-chelatase, highly similar to hypothetical proteins and chelatases e.g. Q9RTV0|DR1656 mg(2+) chelatase family protein from Deinococcus radiodurans (519 aa), FASTA scores: opt: 1333, E(): 3.6e-68, (46.55% identity in 505 aa overlap);Q55372|SLR0904 hypothetical 55.1 KDA protein from Synechocystis sp. strain PCC 6803 (509 aa), FASTA scores: opt: 1271, E(): 1.2e-64, (42.65% identity in 504 aa overlap); Q9HTR4|PA5290 hypothetical protein from Pseudomonas aeruginosa (497 aa), FASTA scores: opt: 1248, E(): 2.3e-63, (45.9% identity in 503 aa overlap); Q9K0Z6|comm|NMB0405 competence protein (mg-chelatase) from Neisseria meningitidis (serogroup B), FASTA scores: opt: 1229, E(): 2.8e-62, (43.2% identity in 509 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). P9WPR1 No conserved hypotheticals P68909 P9WPR1 GO:0015995,GO:0016851,GO:0000166,GO:0006508,GO:0004252,GO:0004176 P9WPR1 NC_000962.3 Mycobrowser_v5 CDS 3207942 3208328 . - 0 Rv2898c Rv2898c Function unknown Conserved hypothetical protein Rv2898c, (MTCY274.29c), len: 128 aa. Conserved hypothetical protein, highly similar to O33024|YS98_MYCLE|ML1607|MLCB250.49 hypothetical 11.0 KDA protein from Mycobacterium leprae (96 aa), FASTA scores: opt: 318, E(): 2.3e-16, (58.35% identity in 96 aa overlap). Also similar to other hypothetical proteins e.g. O69890|YE19_STRCO|SC2E1.19 from Streptomyces coelicolor (130 aa), FASTA scores: opt: 253, E(): 1.7e-11, (39.65% identity in 121 aa overlap); Q9HVZ1|PA4424 from Pseudomonas aeruginosa (125 aa), FASTA scores: opt: 234, E(): 4.2e-10, (40.85% identity in 115 aa overlap); O86871 from Streptomyces lividans (85 aa), FASTA scores: opt: 224, E(): 1.8e-09, (46.45% identity in 84 aa overlap); etc. Equivalent to AAK47292 from Mycobacterium tuberculosis strain CDC1551 (141 aa) but shorter 13 aa. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WFM9 No conserved hypotheticals P67230 P9WFM9 GO:0003677 P9WFM9 NC_000962.3 Mycobrowser_v5 CDS 3209406 3211745 . - 0 Rv2900c fdhF Decomposes formic acid to hydrogen and carbon dioxide under anaerobic conditions in the absence of exogenous electron acceptors [catalytic activity: formate + NAD(+) = CO(2) + NADH]. Possible formate dehydrogenase H FdhF (formate-hydrogen-lyase-linked, selenocysteine-containing polypeptide) (formate dehydrogenase-H alpha subunit) (FDH-H) Rv2900c, (MTCY274.31c), len: 779 aa. Possible fdhF, formate dehydrogenase, highly similar to others formate dehydrogenases and prokaryotic molybdopterin-containing oxidoreductases e.g. Q9S2J9|SC7H2.18 putative formate dehydrogenase from Streptomyces coelicolor (759 aa), FASTA scores: opt: 3038, E(): 2.7e-180, (59.7% identity in 767 aa overlap); Q9HU08|PA5181 probable oxidoreductase from Pseudomonas aeruginosa (773 aa), FASTA scores: opt: 2560, E(): 1.1e-150, (53.2% identity in 761 aa overlap); P78160 formate dehydrogenase a chain (fragment) from Escherichia coli strain K12 (740 aa), FASTA scores: opt: 2002, E(): 3.7e-116, (43.1% identity in 733 aa overlap); P07658|FDHF_ECOLI|P78137|B4079 formate dehydrogenase from Escherichia coli strain K12 (715 aa), FASTA scores: opt: 305, E(): 5.6e-13, (25.5% identity in 748 aa overlap); etc. Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. P9WJP9 No intermediary metabolism and respiration P65408 P9WJP9 GO:0008863,GO:0030151,GO:0055114,GO:0051539 P9WJP9 NC_000962.3 Mycobrowser_v5 CDS 3215665 3216357 . - 0 Rv2906c trmD Specifically methylates guanosime-37 in various TRNAS [catalytic activity S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N1-methylguanine]. Probable tRNA (guanine-N1)-methyltransferase TrmD (M1G-methyltransferase) (tRNA [GM37] methyltransferase) Rv2906c, (MTCY274.37c), len: 230 aa. Probable trmD, tRNA m1G methyltransferase, equivalent to O33017|TRMD_MYCLE from Mycobacterium leprae (238 aa), FASTA scores: opt: 1363, E(): 8.1e-86, (87.2% identity in 227 aa overlap). Also highly similar to others e.g. O69882|TRMD_STRCO from Streptomyces coelicolor and S. lividans (277 aa), FASTA scores: opt: 841, E(): 4.5e-50, (55.55% identity in 234 aa overlap); Q9A0B6 from Streptococcus pyogenes (243 aa), FASTA scores: opt: 698, E(): 2.5e-40, (47.6% identity in 227 aa overlap); P07020|TRMD_ECOLI|TRMD|B2607|Z3901|ECS3470 from Escherichia coli strain O157:H7 (255 aa), FASTA scores: opt: 573, E(): 3.8e-33, (42.1% identity in 228 aa overlap); etc. Belongs to the RNA methyltransferase TRMD family. P9WFY7 No information pathways P66968 P9WFY7 GO:0005737,GO:0009019,GO:0006400,GO:0003723 2.1.1.31 P9WFY7 NC_000962.3 Mycobrowser_v5 CDS 3216361 3216891 . - 0 Rv2907c rimM Essential for efficient processing of 16S rRNA. Probably part of the 30S subunit prior to or during the final step in the processing of 16S free 30S ribosomal subunits. It could be some accessory protein needed for efficient assembly of the 30S subunit. RIMM is needed in a step prior to RBFA during the maturation of 16S rRNA. Has affinity for free ribosomal 30S subunits but not for 70S ribosomes. Probable 16S rRNA processing protein RimM Rv2907c, (MTCY274.38c), len: 176 aa. Probable rimM, 16S rRNA processing protein, equivalent to O33016|RIMM_MYCLE probable 16S rRNA processing protein from Mycobacterium leprae (179 aa), FASTA scores: opt: 797, E(): 2.4e-46, (73.15% identity in 175 aa overlap). Also highly similar to others e.g. O69881|RIMM_STRCO from Streptomyces coelicolor (188 aa), FASTA scores: opt: 485, E(): 2.3e-25, (48.85% identity in 176 aa overlap); Q9KA14|RIMM_BACHD from Bacillus halodurans (173 aa), FASTA scores: opt: 289, E(): 3.2e-12, (30.65% identity in 173 aa overlap); P21504|RIMM_ECOLI|RIMM|B2608 from Escherichia coli strain K12 (182 aa), FASTA scores: opt: 237, E(): 1e-08, (29.4% identity in 177 aa overlap). Belongs to the RimM family. P9WH19 No information pathways P66653 P9WH19 GO:0042274,GO:0005840,GO:0043022,GO:0006364 P9WH19 NC_000962.3 Mycobrowser_v5 CDS 3218339 3219214 . + 0 Rv2911 dacB2 Involved in peptidoglycan synthesis (at final stages). Hydrolyzes the bound D-alanyl-D-alanine [catalytic activity: D-alanyl-D-alanine + H(2)O = 2 D-alanine]. Probable penicillin-binding protein DacB2 (D-alanyl-D-alanine carboxypeptidase) (DD-peptidase) (DD-carboxypeptidase) (PBP) (DD-transpeptidase) (serine-type D-ala-D-ala carboxypeptidase) (D-amino acid hydrolase) Rv2911, (MTCY274.43), len: 291 aa. Probable dacB2, D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein), an ala-rich protein. Highly similar (except in N-terminus) to Q9CCM2|ML0691 putative D-alanyl-D-alanine carboxypeptidase from Mycobacterium leprae (411 aa), FASTA scores: opt: 749, E(): 9.3e-39, (46.75% identity in 276 aa overlap). Also similar to penicillin binding proteins / D-alanyl-D-alanine carboxypeptidases e.g. Q9KCJ8|SC4G1.16c D-alanyl-D-alanine carboxypeptidase from Streptomyces coelicolor (382 aa), FASTA scores: opt: 386, E(): 2.1e-16, (31.25% identity in 285 aa overlap); P35150|DACB_BACSU penicillin-binding protein 5* precursor from Bacillus subtilis (382 aa), FASTA scores: opt: 384, E(): 3.6e-17, (30.7% identity in 244 aa overlap); Q9K8X5|DACB|BH2877 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) from Bacillus halodurans (395 aa), FASTA scores: opt: 359, E(): 9.7e-15, (30.3% identity in 241 aa overlap); P33364|PBP7_ECOLI|PBPG|B2134 penicillin-binding protein 7 precursor from Escherichia coli strain K12 (313 aa), FASTA scores: opt: 273, E(): 7.5e-10, (27.8% identity in 263 aa overlap); etc. Also similar to O53380|Rv3330|MTV016.30 penicillin-binding protein from Mycobacterium tuberculosis (405 aa), FASTA scores: opt: 746, E(): 1.4e-38, (47.0% identity in 266 aa overlap). Seems to contain PF00768 Peptidase_S11 domain PFAM. Belongs to peptidase family S11; also known as the D-alanyl-D-alanine carboxypeptidase 1 family. Thought to be a membrane-bound protein. Note that previously known as dacB. I6Y204 No cell wall and cell processes 2BCF Q7D6F2 I6Y204 GO:0009002,GO:0006508 3.4.16.4 I6Y204 NC_000962.3 Mycobrowser_v5 CDS 3219863 3221698 . - 0 Rv2913c Rv2913c Hydrolyzes specific D-amino acid. Possible D-amino acid aminohydrolase (D-amino acid hydrolase) Rv2913c, (MTCY338.01c, MTCY274.45c), len: 611 aa. Possible D-amino acid aminohydrolase, similar (principally in N-terminus) to D-amino acid aminohydrolases e.g. Q9V2D3|NDAD|PAB0090 D-aminoacylase (aspartate, glutamate etc) from Pyrococcus abyssi (526 aa), FASTA scores: opt: 336, E(): 2.2e-13, (27.55% identity in 581 aa overlap); P94212|NDDD_ALCXX N-acyl-D-aspartate deacylase (N-acyl-D-aspartate amidohydrolase) from Alcaligenes xylosoxydans xylosoxydans (Achromobacter xylosoxidans) (498 aa), FASTA scores: opt: 221, E(): 3.4e-06, (25.95% identity in 532 aa overlap); Q9AGH8 D-aminoacylase from Alcaligenes faecalis (484 aa), FASTA scores: opt: 218, E(): 5.1e-06, (28.35% identity in 434 aa overlap); etc. P9WJH9 No intermediary metabolism and respiration P65530 P9WJH9 GO:0016810 P9WJH9 NC_000962.3 Mycobrowser_v5 CDS 3385163 3386077 . - 0 Rv3026c Rv3026c Function unknown Conserved hypothetical protein Rv3026c, (MTV012.41c), len: 304 aa. Conserved hypothetical protein, similar to Q9RCZ0|SCM10.08C putative acyltransferase from Streptomyces coelicolor (275 aa), FASTA scores: opt: 393, E(): 2.2e-17, (41.4% identity in 299 aa overlap). Similar in part to other hypothetical proteins and acyltransferases e.g. BAB51968|MLR5533 from Rhizobium loti (266 aa), FASTA scores: opt: 280, E(): 2.4e-10, (29.45% identity in 258 aa overlap); Q9KIH9 putative acyltransferase (putative acyltransferase transmembrane protein) from Rhizobium meliloti (Sinorhizobium meliloti) (292 aa), FASTA scores: opt: 252, E(): 1.4e-08, (30.5% identity in 210 aa overlap); O69114|PLSC putative 1-acyl-SN-glycerol-3-phosphate acyltransferase from Burkholderia pseudomallei (Pseudomonas pseudomallei) (289 aa), FASTA scores: opt: 216, E(): 2.4e-06, (30.85% identity in 269 aa overlap); etc. So may be a member of acyltransferase family protein. I6XFY8 No conserved hypotheticals O53273 I6XFY8 GO:0008152,GO:0008415 I6XFY8 NC_000962.3 Mycobrowser_v5 CDS 3226363 3228243 . + 0 Rv2917 Rv2917 Function unknown Conserved hypothetical alanine and arginine rich protein Rv2917, (MTCY338.05), len: 626 aa. Conserved hypothetical ala-, arg-rich protein, highly similar (but longer 34 aa) to O33011|ML1624|MLCB250.18C hypothetical 65.2 KDA protein from Mycobacterium leprae (596 aa), FASTA scores: opt: 3117, E(): 9e-183, (79.8% identity in 584 aa overlap). Also highly similar to Q9S2E8|SCE19A.36C hypothetical 66.2 KDA protein from Streptomyces coelicolor (598 aa), FASTA scores: opt: 1921, E(): 1.1e-109, (56.08% identity in 567 aa overlap); and Q9S3Y6|SDRA SDRA protein from Streptomyces coelicolor (597 aa), FASTA scores: opt: 1896, E(): 3.6e-108, (55.75% identity in 567 aa overlap). And shows some similarity with others proteins from other organisms. Equivalent to AAK47311 putative RNA helicase from Mycobacterium tuberculosis strain CDC1551 (602 aa) but longer 24 aa. Contains PS00017 ATP/GTP-binding site motif A (P-loop). P9WL21 No conserved hypotheticals Q10966 P9WL21 GO:0003677,GO:0017111,GO:0005524 P9WL21 NC_000962.3 Mycobrowser_v5 CDS 3228254 3230680 . - 0 Rv2918c glnD Modifies, by uridylylation or deuridylylation the PII (GLNB|Rv2919c) regulatory protein [catalytic activity: UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII]]. Probable [protein-PII] uridylyltransferase GlnD (PII uridylyl- transferase) (uridylyl removing enzyme) (UTASE) Rv2918c, (MTCY338.07c), len: 808 aa. Probable glnD, uridylyltransferase (ala-rich protein), similar to other uridylyltransferases e.g. O69873||SC2E1.02 from Streptomyces coelicolor (835 aa), FASTA scores: opt: 1473, E(): 2.8e-81, (41.03% identity in 858 aa overlap); P43919|GLND_HAEIN from Haemophilus influenzae (863 aa), FASTA scores: opt: 333, E(): 2.5e-12, (25.4% identity in 819 aa overlap); P27249|GLND_ECOLI|GLND|B0167 from Escherichia coli strain K12 (890 aa), FASTA scores: opt: 306, E(): 1.1e-10, (27.75% identity in 858 aa overlap); etc. Belongs to the GlnD family. P9WN29 No intermediary metabolism and respiration Q10961 P9WN29 GO:0016597,GO:0006807,GO:0008773 2.7.7.59 P9WN29 NC_000962.3 Mycobrowser_v5 CDS 3231073 3232506 . - 0 Rv2920c amt Thought to be involved in transport of ammonium across the membrane (export). Responsible for the translocation of the substrate across the membrane. Probable ammonium-transport integral membrane protein Amt Rv2920c, (MTCY338.09c), len: 477 aa. Probable amt, ammonium-transport integral membrane protein (ala-, gly-, leu-, val-rich protein), highly similar to others e.g. Q9ZBP6|SC7A1.27 ammonium transporter from Streptomyces coelicolor (448 aa), FASTA scores: opt: 1246, E(): 7.3e-67, (54.1% identity in 462 aa overlap); P54146|AMT_CORGL ammonium transport system from Corynebacterium glutamicum (452 aa), FASTA scores: opt: 953, E(): 2.1e-49, (41.45% identity in 475 aa overlap); Q07429|NRGA_BACSU probable ammonium transporter (membrane protein NRGA) from Bacillus subtilis (404 aa), FASTA scores: opt: 721, E(): 0, (44.4% identity in 430 aa overlap); etc. Belongs to the AMT1/MEP/NRGA family of ammonium transporters (TC 2.49). P9WQ65 No cell wall and cell processes P63519 P9WQ65 GO:0015696,GO:0016021,GO:0005886,GO:0055085,GO:0008519 P9WQ65 NC_000962.3 Mycobrowser_v5 CDS 3232871 3234139 . - 0 Rv2921c ftsY Probably involved in the reception and insertion of a subset of proteins at the membrane: possibly membrane receptor for FFH|Rv2916c. Probable cell division protein FtsY (SRP receptor) (signal recognition particle receptor) Rv2921c, (MTCY338.10c, MT2989), len: 422 aa. Probable ftsY, signal recognition particle (SRP) receptor, a membrane-associated cell division protein (see citation below), equivalent to O33010|FTSY_MYCLE cell division protein FTSY homolog from Mycobacterium leprae (430 aa), FASTA scores: opt: 1760, E(): 1.1e-108, (81.35% identity in 429 aa overlap). Also similar to others e.g. Q9I6C1|FTSY|PA0373 signal recognition particle receptor FTSY from Pseudomonas aeruginosa (455 aa), FASTA scores: opt: 882, E(): 5.1e-40, (42.08% identity in 385 aa overlap); Q9KVJ6|FTSY cell division protein from Vibrio cholerae (391 aa), FASTA scores: opt: 837, E(): 1.2e-37, (36.3% identity in 394 aa overlap); P10121|FTSY_ECOLI|FTSY|B3464 cell division protein from Escherichia coli strain K12 (497 aa), FASTA scores: opt: 800, E(): 1.3e-35, (39.75% identity in 327 aa overlap); etc. Also similar to Q9ZBP9|SC7A1.24 putative prokaryotic docking protein from Streptomyces coelicolor (412 aa), FASTA scores: opt: 1461, E(): 4.3e-71, (60.3% identity in 423 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00300 SRP54-type proteins GTP-binding domain signature. Belongs to the SRP family of GTP-binding proteins. P9WGD9 No cell wall and cell processes P66842 P9WGD9 GO:0003723,GO:0006614,GO:0007049,GO:0051301,GO:0017111,GO:0005886,GO:0005515,GO:0030529,GO:0005525 P9WGD9 NC_000962.3 Mycobrowser_v5 CDS 3241222 3241959 . - 0 Rv2927c Rv2927c Function unknown Conserved hypothetical protein Rv2927c, (MTCY338.16c), len: 245 aa. Conserved hypothetical protein, equivalent to Q9CBS6|ML1661|MLCB1243.13 (alias O69467) hypothetical protein from Mycobacterium leprae (247 aa), FASTA scores: opt: 1440, E(): 4.9e-76, (90.6% identity in 245 aa overlap). Also similar to many hypothetical proteins from other organisms e.g. Q9ZBR0|SC7A1.13 hypothetical 41.0 KDA protein from Streptomyces coelicolor (379 aa), FASTA scores: opt: 266, E(): 3.4e-08, (29.9% identity in 234 aa overlap); etc. Also some similarity with P46815|AG84_MYCLE|ML0922 antigen 84 from Mycobacterium leprae (266 aa), FASTA scores: opt: 193, E(): 0.00043, (28.7% identity in 136 aa overlap) (see citation below); and P46816|AG84_MYCTU|WAG31|Rv2145c|MT2204|MTCY270.23 antigen 84 from Mycobacterium tuberculosis (260 aa), FASTA scores: opt: 178, E(): 0.0031, (34.35% identity in 131 aa overlap) (see citation below). Contains potential coiled-coil region. P9WL15 No conserved hypotheticals P9WL15 P9WL15 NC_000962.3 Mycobrowser_v5 CDS 3272214 3273209 . + 0 Rv2936 drrA Probably involved in active transport of antibiotic and phthiocerol dimycocerosate (dim) across the membrane (export). DRRA, DRRB|Rv2936|MTCY19H9.05 and DRRC|Rv2938|MTCY19H9.06 may act jointly to confer daunorubicin and doxorubicin resistance by an export mechanism. Responsible for energy coupling to the transport system. Daunorubicin-dim-transport ATP-binding protein ABC transporter DrrA Rv2936, (MTCY19H9.04), len: 331 aa. drrA, daunorubicin-dim-transport resistance ATP-binding protein ABC transporter, probably involved in daunorubicin resistance and phthiocerol dimycocerosate transport (see citations below), equivalent to Q49938|DRRA|ML2352|L518_F2_43|DRRA probable daunorubicin resistance ATP-binding protein from Mycobacterium leprae (331 aa), FASTA scores: opt: 1842, E(): 4.2e-103, (85.2% identity in 331 aa overlap). Also highly similar to others e.g. Q9XCF7 DRRA from Mycobacterium avium (315 aa), FASTA scores: opt: 1040, E(): 4.7e-55, (54.35% identity in 309 aa overlap); Q9X5J8 daunorubicin resistance protein A from Mycobacterium avium (315 aa), FASTA scores: opt: 1030, E(): 1.9e-54, (53.7% identity in 309 aa overlap); P32010|DRRA_STRPE daunorubicin resistance ATP-binding protein from Streptomyces peucetius (330 aa), FASTA scores: opt: 852, E(): 9e-44, (47.15% identity in 318 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). Note that Rv2936|drrA belongs to the transcriptional unit Rv2930|fadD26-Rv2939|papA5 (proven experimentally). P9WQL9 No cell wall and cell processes P96205 P9WQL9 GO:0016887,GO:0005886,GO:0046677,GO:0055085,GO:0005524 3.6.3.- P9WQL9 NC_000962.3 Mycobrowser_v5 CDS 3274072 3274902 . + 0 Rv2938 drrC Probably involved in active transport of antibiotic and phthiocerol dimycocerosate (dim) across the membrane (export). DRRA|Rv2934|MTCY19H9.04, DRRB|Rv2937|MTCY19H9.05 and DRRC may act jointly to confer daunorubicin and doxorubicin resistance by an export mechanism. Probably responsible for the translocation of the substrate across the membrane and localization of dim into the cell wall. Probable daunorubicin-dim-transport integral membrane protein ABC transporter DrrC Rv2938, (MTCY19H9.06), len: 276 aa. Probable drrC, daunorubicin-dim-transport integral membrane protein ABC transporter, probably involved in daunorubicin resistance and phthiocerol dimycocerosate transport (see citations below), equivalent to Q9CB71|ML2350 probable antibiotic resistance membrane protein from Mycobacterium leprae (276 aa), FASTA scores: opt: 1434, E(): 1.2e-81, (79.0% identity in 276 aa overlap); and Q49941|DRRC|L518_F3_76 putative daunorubicin resistance transmembrane protein from Mycobacterium leprae (244 aa), FASTA scores: opt: 1194, E(): 8.3e-67, (76.85% identity in 242 aa overlap). Also similar to others e.g. Q9XCF9 DRRC protein from Mycobacterium avium (263 aa), FASTA scores: opt: 538, E(): 3.7e-26, (32.65% identity in 251 aa overlap); Q9S6H3 daunorubicin resistance protein C from Mycobacterium avium (263 aa), FASTA scores: opt: 533, E(): 7.6e-26, (32.25% identity in 251 aa overlap); P32011|DRRB_STRPE daunorubicin resistance transmembrane protein from Streptomyces peucetius (283 aa), FASTA scores: opt: 276, E(): 6.6e-10, (21.07% identity in 261 aa overlap); etc. Note that Rv2938|drrC belongs to the transcriptional unit Rv2930|fadD26-Rv2939|papA5 (proven experimentally). P9WG21 No cell wall and cell processes P96207 P9WG21 GO:0042626,GO:0016021,GO:0005886,GO:0046677,GO:0006810,GO:0005524 NC_000962.3 Mycobrowser_v5 CDS 3283335 3285077 . + 0 Rv2941 fadD28 Involved in phthiocerol dimycocerosate (dim) biosynthesis. Thought to be involved in the release and transfer of mycoserosic acid from mas onto the DIOLS. Fatty-acid-AMP ligase FadD28 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) Rv2941, (MTCY24G1.08c), len: 580 aa. FadD28 (alternate gene name: acoas), fatty-acid-AMP synthetase (see citations below), almost identical to P71495 acyl-CoA synthase from Mycobacterium bovis (582 aa), FASTA scores: opt: 3828, E(): 0, (99.15% identity in 580 aa overlap); and equivalent to Q9CD79|FADD28|ML0138 acyl-CoA synthetase from Mycobacterium leprae (579 aa), FASTA scores: opt: 3183, E(): 8.8e-186, (81.9% identity in 580 aa overlap). And also highly similar to others Mycobacteria proteins e.g. O07797|FADD23|Rv3826|MTCY409.04c putative fatty-acid-CoA synthetase from Mycobacterium tuberculosis (584 aa); etc. Contains PS00018 EF-hand calcium-binding domain. Note that Rv2941|fadD28 and Rv2942|mmpL7 are transcriptionally coupled (proven experimentally). P9WQ59 No lipid metabolism 3E53 P96290 P9WQ59 GO:0008152,GO:0016874 6.2.1.- P9WQ59 NC_000962.3 Mycobrowser_v5 CDS 3350274 3350993 . - 0 Rv2993c Rv2993c Isomerizes HHDD (2-hydroxy-hept-2,4-diene-1,7-dioate) to OHED (2-oxo-hept-3-ene-1,7-dioate). Possible 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (HHDD isomerase) Rv2993c, (MTV012.07c), len: 239 aa. Possible 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, equivalent to O33119|ML1689|MLCB637.28 possible 2-hydroxyhepta-2,4-diene- 1,7-dioate isomerase from Mycobacterium leprae (242 aa), FASTA scores: opt: 1427, E(): 4.4e-86, (85.9% identity in 241 aa overlap). Also similar to others e.g. Q9LBE3|DR1609 from Deinococcus radiodurans (250 aa), FASTA scores: opt: 723, E(): 5.5e-40, (49.05% identity in 216 aa overlap); O27551|MTH1507 from Methanothermobacter thermautotrophicus (260 aa), FASTA scores: opt: 708, E(): 5.4e-39, (52.1% identity in 213 aa overlap); Q9HQR6|VNG1037G|HPCE from Halobacterium sp. (strain NRC-1) (244 aa), FASTA scores: opt: 590, E(): 2.7e-31, (43.65% identity in 220 aa overlap); etc. Start chosen by homology, but ORF could continue upstream. I6Y276 No intermediary metabolism and respiration O53242 I6Y276 GO:0008152,GO:0016853 5.3.3.- I6Y276 NC_000962.3 Mycobrowser_v5 CDS 3290624 3291325 . - 0 Rv2945c lppX Unknown Probable conserved lipoprotein LppX Rv2945c, (MTCY24G1.04), len: 233 aa. Probable lppX, conserved lipoprotein, equivalent to Q9CD80 putative lipoprotein from Mycobacterium leprae (233 aa), FASTA scores: opt: 1165, E(): 2.1e-65, (76.4% identity in 233 aa overlap); and similar to Q9CCP6|ML0557 from Mycobacterium leprae (238 aa), FASTA scores: opt: 338, E(): 7.4e-14, (30.75% identity in 231 aa overlap). Also similar to others from Mycobacterium tuberculosis e.g. P71679|LPRG_MYCTU lipoprotein (236 aa), FASTA scores: opt: 342, E(): 4.1e-14, (32.05% identity in 231 aa overlap); etc. Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site, and has in its N-terminal a signal peptide. Belongs to the LPPX/lprafg family of lipoproteins. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P9WK65 No cell wall and cell processes 2BYO P65306 P9WK65 GO:0005886 P9WK65 NC_000962.3 Mycobrowser_v5 CDS 3297837 3299954 . - 0 Rv2948c fadD22 Involved in biosynthesis of phenolic glycolipids (PGLs) P-hydroxybenzoyl-AMP ligase FadD22 Rv2948c, (MTCY24G1.01), len: 705 aa. FadD22, p-hydroxybenzoyl-AMP ligase. Highly similar to many e.g. Q9CD82|ML0134 putative acyl-CoA synthetase from Mycobacterium leprae (707 aa), fasta scores: opt: 3554, E(): 6.4e-209, (75.9% identity in 705 aa overlap). Almost identical to G560509|Q50468 PKS002A protein from Mycobacterium tuberculosis (705 aa), fasta scores: opt: 4647, E(): 0, (99.7% identity in 705 aa overlap). P9WQ61 No lipid metabolism P96283 P9WQ61 GO:0048037,GO:0016874,GO:0008152,GO:0000036 6.2.1.- P9WQ61 NC_000962.3 Mycobrowser_v5 CDS 3300596 3302455 . - 0 Rv2950c fadD29 Involved in biosynthesis of phenolic glycolipids (PGLs) Fatty-acid-AMP ligase FadD29 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) Rv2950c, (MTCY349.40), len: 619 aa. fadD29, fatty-acid-AMP synthetase, similar to various mycobacterial enzymes believed to be involved in polyketide or fatty acid synthesis. Equivalent (but shorter 61 aa) to Q9CD84 from Mycobacterium leprae (680 aa), FASTA scores: opt: 3280, E(): 2.2e-192, (80.15% identity in 620 aa overlap); and highly similar to others from Mycobacterium leprae e.g. Q9Z5K5 probable acyl-CoA synthase (583 aa), FASTA scores: opt: 2358, E(): 3.4e-136, (62.35% identity in 579 aa overlap). Also similar to others from Mycobacterium tuberculosis e.g. Q10976|FD26_MYCTU putative fatty-acid--CoA ligase (583 aa), FASTA scores: opt: 2416, E(): 1e-139, (63.15% identity in 581 aa overlap) (N-terminus shorter); etc. Equivalent to AAK47349 from Mycobacterium tuberculosis strain CDC1551 (582 aa) but longer 37 aa. P95141 No lipid metabolism P95141 P95141 GO:0008152,GO:0016874 6.2.1.- P95141 NC_000962.3 Mycobrowser_v5 CDS 3304441 3305253 . + 0 Rv2952 Rv2952 Thought to cause methylation. Possible methyltransferase (methylase) Rv2952, (MTCY349.38), len: 270 aa. Probable methyltransferase, equivalent to Q9CD86|ML0130 hypothetical protein from Mycobacterium leprae (270 aa), FASTA scores: opt: 1584, E(): 6.1e-99, (83.7% identity in 270 aa overlap). Also highly similar to Q9RMN9|MTF2 putative methyltransferase from Mycobacterium smegmatis (274 aa), FASTA scores: opt: 902, E(): 3.8e-53, (56.35% identity in 252 aa overlap). Also similar to other methyltransferases e.g. Q9ADL4|SORM O-methyltransferase from Polyangium cellulosum (346 aa), FASTA scores: opt: 390, E(): 1.1e-18, (36.25% identity in 251 aa overlap); Q54303|RAPM methyltransferase from Streptomyces hygroscopicus (317 aa), FASTA scores: opt: 315, E(): 1.1e-13, (40.75% identity in 135 aa overlap); etc. Very similar to C-terminal part of Q50584|Rv1523|MTCY19G5.05c hypothetical 37.9 KDA protein from Mycobacterium tuberculosis (358 aa), FASTA score: opt: 965, E(): 2.7e-57, (60.3% identity in 247 aa overlap). P9WIN3 No intermediary metabolism and respiration Q50464 P9WIN3 GO:0008168,GO:0008610 2.1.1.- P9WIN3 NC_000962.3 Mycobrowser_v5 CDS 3310714 3312000 . - 0 Rv2958c Rv2958c Function unknown; probably involved in cellular metabolism. Possibly involved in resistance to killing by human macrophages. Possible glycosyl transferase Rv2958c, (MTCY349.30), len: 428 aa. Possible glycosyl transferase (see citation below), highly similar to Q9CD88|ML0128 putative glycosyl transferase from Mycobacterium leprae (435 aa), FASTA scores: opt: 2116, E(): 5.8e-126, (75.05% identity in 417 aa overlap); and Q9CD91|ML0125 putative glycosyl transferase from Mycobacterium leprae (438 aa), FASTA scores: opt: 2104, E(): 3.3e-125, (74.65% identity in 418 aa overlap). Also shows some similarity to variety of glycosyl transferases e.g. Q9RYI3 putative glycosyltransferase from Deinococcus radiodurans (418 aa), FASTA scores: opt: 317, E(): 1.9e-12, (31.0% identity in 297 aa overlap); Q9S1V2 putative glycosyl transferase from Streptomyces coelicolor (407 aa), FASTA scores: opt: 264, E(): 4.1e-09, (27.2% identity in 342 aa overlap); P72650|CRTX|SLR1125 zeaxanthin glucosyl transferase from Synechocystis sp. strain PCC 6803 (419 aa), FASTA scores: opt: 251, E(): 2.8e-08, (26.8% identity in 295 aa overlap); etc. Very similar to P95130|MTCY349.25 from Mycobacterium tuberculosis (449 aa), FASTA score: opt: 2215, E(): 3.3e-132, (77.25% identity in 422 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WFR1 No intermediary metabolism and respiration P95134 P9WFR1 GO:0016758,GO:0008152 2.4.1.- P9WFR1 NC_000962.3 Mycobrowser_v5 CDS 3313773 3315122 . - 0 Rv2962c Rv2962c Function unknown; probably involved in cellular metabolism. Possibly involved in resistance to killing by human macrophages. Possible glycosyl transferase Rv2962c, (MTCY349.25), len: 449 aa. Possible glycosyl transferase (see citation below), highly similar or identical to Mycobacterium tuberculosis proteins G560522 U0002JA, G560521 U0002H, G560522 U0002JA, G560519 U0002KA. Equivalent (but longer 21 aa) to Q9CD91 putative glycosyl transferase from Mycobacterium leprae (438 aa), FASTA scores: opt: 2229, E(): 1.3e-133, (77.45% identity in 426 aa overlap); and highly similar to Q9CD88 putative glycosyl transferase from Mycobacterium leprae (435 aa), FASTA scores: opt: 2129, E(): 2.7e-127, (74.35% identity in 425 aa overlap); and others from Mycobacterium leprae. Also shows some similarity to variety of glycosyl transferases e.g. Q9RYI3|DRA0329 putative glycosyl transferase from Deinococcus radiodurans (418 aa), FASTA scores: opt: 340, E(): 5.5e-14, (31.2% identity in 330 aa overlap); P72650 zeaxanthin glucosyl transferase from Synechocystis sp. (strain PCC 6803) (419 aa), FASTA scores: opt: 244, E(): 6.6e-08, (26.2% identity in 294 aa overlap); etc. Also highly similar to P95134 hypothetical 46.8 KDA protein from Mycobacterium tuberculosis (428 aa), FASTA scores: opt: 2215, E(): 9.6e-133, (77.25% identity in 422 aa overlap). P9WN09 No intermediary metabolism and respiration P95130 P9WN09 GO:0016758,GO:0008152 2.4.1.- P9WN09 NC_000962.3 Mycobrowser_v5 CDS 3316529 3317461 . + 0 Rv2964 purU Involved in de novo purine biosynthesis [catalytic activity: 10-formyltetrahydrofolate + H(2)O = formate + tetrahydrofolate]. Probable formyltetrahydrofolate deformylase PurU (formyl-FH(4) hydrolase) Rv2964, (MTCY349.23c), len: 310 aa. Probable purU, formyltetrahydrofolate deformylase, highly similar to others e.g. Q9RWT1|DR0584 formyltetrahydrofolate deformylase from Deinococcus radiodurans (298 aa), FASTA scores: opt: 1005, E(): 4.9e-52, (52.25% identity in 297 aa overlap); Q9K7U4 formyltetrahydrofolate deformylase from Bacillus halodurans (289 aa), FASTA scores: opt: 982, E(): 1.1e-50, (51.8% identity in 280 aa overlap); Q55135|PURU_SYNY3|SLL0070 formyltetrahydrofolate deformylase from Synechocystis sp. strain PCC 6803 (284 aa), FASTA scores: opt: 839, E(): 2.9e-42, (48.2% identity in 280 aa overlap); etc. P9WHM3 No intermediary metabolism and respiration P0A5T6 P9WHM3 GO:0016597,GO:0008864,GO:0016742,GO:0006730,GO:0006189 3.5.1.10 P9WHM3 NC_000962.3 Mycobrowser_v5 CDS 3319663 3323046 . - 0 Rv2967c pca Involved in gluconeogenesis and lipogenesis. Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [catalytic activity: ATP + pyruvate + HCO(3)(-) = ADP + phosphate + oxaloacetate]. Probable pyruvate carboxylase Pca (pyruvic carboxylase) Rv2967c, (MTCY349.20), len: 1127 aa. Probable pca, pyruvate carboxylase (ala-rich protein), equivalent to Q9F843|PYC pyruvate carboxylase from Mycobacterium smegmatis (1127 aa), FASTA scores: opt: 6232, E(): 0, (83.3% identity in 1127 aa overlap). Also highly similar to others e.g. Q9RK64|SCF11.26c pyruvate carboxylase from Streptomyces coelicolor (1124 aa), FASTA scores: opt: 5526, E(): 0, (74.65% identity in 1125 aa overlap); O54587|PYC pyruvate carboxylase from Corynebacterium glutamicum (Brevibacterium flavum) (1140 aa), FASTA scores: opt: 4811, E(): 0, (64.5% identity in 1132 aa overlap); Q9DDT1 pyruvate carboxylase from Brachydanio rerio (Zebrafish) (1180 aa), FASTA scores: opt: 3133, E(): 1.1e-171, (47.8% identity in 1142 aa overlap); etc. Contains PS00867 Carbamoyl-phosphate synthase subdomain signature 2, PS00165 Serine/threonine dehydratases pyridoxal-phosphate attachment site, and PS00188 Biotin-requiring enzymes attachment site. I6YEU0 No intermediary metabolism and respiration P95127 I6YEU0 GO:0009374,GO:0005737,GO:0006094,GO:0004736,GO:0005524 6.4.1.1 I6YEU0 NC_000962.3 Mycobrowser_v5 CDS 3323709 3324476 . - 0 Rv2969c Rv2969c Unknown Possible conserved membrane or secreted protein Rv2969c, (MTCY349.18), len: 255 aa. Possible conserved membrane or exported protein, equivalent to Q9CBS4|ML1667 possible conserved membrane protein from Mycobacterium leprae (264 aa), FASTA scores: opt: 1101, E(): 9.9e-68, (65.9% identity in 258 aa overlap); and highly similar to O69463 putative transmembrane protein from Mycobacterium leprae (258 aa), FASTA scores: opt: 1097, E(): 1.8e-67, (65.5% identity in 258 aa overlap). C-terminus also highly similar to Q9KK65|996A160 exported protein (fragment) from Mycobacterium avium (85 aa), FASTA scores: opt: 418, E(): 2e-21, (72.95% identity in 85 aa overlap). Also weakly similar to membrane or exported proteins e.g. Q9S2U7|SC4G6.04c putative integral membrane protein from Streptomyces coelicolor (275 aa), FASTA scores: opt: 312, E(): 7.6e-14, (28.25% identity in 230 aa overlap); Q9XAB6|SCC22.22C putative secreted protein from Streptomyces coelicolor (255 aa), FASTA scores: opt: 181, E(): 6.4e-05, (27.0% identity in 226 aa overlap); etc. Also some similarity with P72001|PKNE_MYCTU from Mycobacterium tuberculosis (566 aa), FASTA scores: opt: 264, E(): 2.3e-10, (30.5% identity in 177 aa overlap). O33272 No cell wall and cell processes O33272 O33272 NC_000962.3 Mycobrowser_v5 CDS 3326101 3326949 . + 0 Rv2971 Rv2971 Function unknown; probably involved in cellular metabolism. Probable oxidoreductase Rv2971, (MTCY349.16c), len: 282 aa. Probable oxidoreductase, possibly aldo/keto reductase, equivalent to O69462 putative oxidoreductase from Mycobacterium leprae (282 aa), FASTA scores: opt: 1495, E(): 4.9e-93, (82.35% identity in 272 aa overlap). Also similar to others e.g. Q9KYM9|SC9H11.10C oxidoreductase from Streptomyces coelicolor (276 aa), FASTA scores: opt: 849, E(): 1.2e-49, (51.7% identity in 267 aa overlap); Q9ZBW7|SC4B5.01C putative oxidoreductase from Streptomyces coelicolor (277 aa), FASTA scores: opt: 847, E(): 1.7e-49, (49.1% identity in 271 aa overlap); Q46857|YQHE_ECOLI|YQHE|B3012 hypothetical oxidoreductase from Escherichia coli strain K12 (275 aa), FASTA scores: opt: 827, E(): 3.7e-48, (47.45% identity in 276 aa overlap); etc. Contains PS00063 Aldo/keto reductase family putative active site signature; and PS00062 Aldo/keto reductase family signature 2. P9WQA5 No intermediary metabolism and respiration P95124 P9WQA5 GO:0016491,GO:0055114 1.-.-.- P9WQA5 NC_000962.3 Mycobrowser_v5 CDS 3327023 3327736 . - 0 Rv2972c Rv2972c Unknown Possible conserved membrane or exported protein Rv2972c, (MTCY349.15), len: 237 aa. Possible conserved membrane or exported protein, equivalent (but longer 52 aa) to O69461|MLCB1243.02 hypothetical 20.5 KDA protein from Mycobacterium leprae (180 aa), FASTA scores: opt: 581, E(): 8.2e-32, (55.75% identity in 174 aa overlap). Also similar to membrane or exported proteins e.g. Q9F2P3|SCE41.16C putative lipoprotein from Streptomyces coelicolor (258 aa), FASTA scores: opt: 498, E(): 4.1e-26, (44.08% identity in 186 aa overlap); Q99QB5|SCP1.323C putative secreted protein from Streptomyces coelicolor (219 aa), FASTA scores: opt: 329, E(): 8.5e-15, (36.35% identity in 176 aa overlap); Q9ACQ1|SCP1.267 putative secreted protein from Streptomyces coelicolor (219 aa), FASTA scores: opt: 286, E(): 6.6e-12, (32.03% identity in 231 aa overlap); etc. I6X5W6 No cell wall and cell processes P95123 I6X5W6 I6X5W6 NC_000962.3 Mycobrowser_v5 CDS 3332071 3332754 . - 0 Rv2976c ung Involved in base excision repair.excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. Probable uracil-DNA glycosylase Ung (UDG) Rv2976c, (MTCY349.11), len: 227 aa. Probable ung, uracil-DNA glycosylase (see citation below), equivalent to Q9CBS3 uracil-DNA glycosylase from Mycobacterium leprae (227 aa), FASTA scores: opt: 1394, E(): 8.8e-85, (88.1% identity in 227 aa overlap). Also highly similar to others e.g. Q9EX12 from Streptomyces coelicolor (225 aa), FASTA scores: opt: 1134, E(): 1.3e-67, (72.75% identity in 224 aa overlap); Q9K682|UNG_BACHD from Bacillus halodurans (224 aa), FASTA scores: opt: 652, E(): 8.9e-36, (45.5% identity in 222 aa overlap); P39615|UNG_BACSU from Bacillus subtilis (225 aa), FASTA scores: opt: 625, E(): 5.4e-34, (45.5% identity in 222 aa overlap); etc. Belongs to the uracil-DNA glycosylase family. P9WFQ9 No information pathways 2ZHX,3A7N P67071 P9WFQ9 GO:0005737,GO:0004844,GO:0006284 3.2.2.27 P9WFQ9 NC_000962.3 Mycobrowser_v5 CDS 3332787 3333788 . - 0 Rv2977c thiL Involved in thiamine biosynthesis [catalytic activity: ATP + thiamine phosphate = ADP + thiamine diphosphate]. Probable thiamine-monophosphate kinase ThiL (thiamine-phosphate kinase) Rv2977c, (MTCY349.10), len: 333 aa. Possible thiL, thiamin-monophosphate kinase, equivalent to Q9CBS2 probable thiamine-monophosphate kinase from Mycobacterium leprae (325 aa), FASTA scores: opt: 1738, E(): 4.5e-98, (80.9% identity in 314 aa overlap). Also highly similar to others e.g. Q9ZBR7|SC7A1.06 putative thiamine monphosphate kinase from Streptomyces coelicolor (322 aa), FASTA scores: opt: 959, E(): 7.8e-51, (51.1% identity in 319 aa overlap); O05514|THIL_BACSU thiamine-monophosphate kinase from Bacillus subtilis (325 aa), FASTA scores: opt: 476, E(): 1.5e-21, (35.15% identity in 273 aa overlap); P77785|THIL_ECOLI|THIL|B0417 thiamine-monophosphate kinase from Escherichia coli strain K12 (325 aa), FASTA scores: opt: 418, E(): 5e-18, (36.9% identity in 282 aa overlap); etc. Belongs to the thiamine-monophosphate kinase family. Note that the start, as given, is in IS1538. P9WG71 No intermediary metabolism and respiration P95118 P9WG71 GO:0009030,GO:0009228 2.7.4.16 P9WG71 NC_000962.3 Mycobrowser_v5 CDS 3336796 3337917 . - 0 Rv2981c ddlA Involved in cell wall formation. Along with alanine racemase, it MAKES up the D-alanine branch of the peptidoglycan biosynthetic route. [catalytic activity: ATP + D-alanine + D-alanine = ADP + phosphate + D-alanyl-D-alanine]. Probable D-alanine--D-alanine ligase DdlA (D-alanylalanine synthetase) (D-ala-D-ala ligase) Rv2981c, (MTCY349.06), len: 373 aa. Probable ddlA (alternate gene name: ddl), D-alanine--D-alanine ligase a (see citation below), equivalent to Q9CBS0|Q9CBS0 D-alanine-D-alanine ligase a from Mycobacterium leprae (384 aa), FASTA scores: opt: 2001, E(): 2.4e-115, (81.75% identity in 367 aa overlap); and Q9ZGN0|DDL_MYCSM D-alanine--D-alanine ligase from Mycobacterium smegmatis (373 aa), FASTA scores: opt: 1934, E(): 3.1e-111, (77.95% identity in 372 aa overlap). Also highly similar to others e.g. Q9ZBR9|DDL_STRCO from Streptomyces coelicolor (389 aa), FASTA scores: opt: 1187, E(): 2.2e-65, (52.0% identity in 379 aa overlap); P15051|DDLA_SALTY from Salmonella typhimurium and Salmonella typhi (363 aa), FASTA scores: opt: 946, E(): 1.3e-50, (44.5% identity in 364 aa overlap); P23844|DDLA_ECOLI|DDLA|B0381|Z0477|ECS0431 from Escherichia coli strain O157:H7 and K12 (364 aa), FASTA scores: opt: 938, E(): 3.9e-50, (43.55% identity in 363 aa overlap); etc. Contains PS00843 D-alanine--D-alanine ligase signature 1. Belongs to the D-alanine--D-alanine ligase family. P9WP31 No cell wall and cell processes 3LWB P95114 P9WP31 GO:0008716,GO:0005618,GO:0007047,GO:0005737,GO:0046872,GO:0009252,GO:0008360,GO:0005524 6.3.2.4 P9WP31 NC_000962.3 Mycobrowser_v5 CDS 3339854 3342082 . + 0 Rv2984 ppk1 Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyp) [catalytic activity: ATP + {phosphate}(N) = ADP + {phosphate}(N+1)]. Likely involved in MPRAB-SIGE-rel signalling and the stringent response. Polyphosphate kinase PPK (polyphosphoric acid kinase) (ATP-polyphosphate phosphotransferase) Rv2984, (MTCY349.03c), len: 742 aa. Ppk1, polyphosphate kinase (See Sureka et al., 2007), equivalent to O33127|PPK_MYCLE polyphosphate kinase from Mycobacterium leprae (739 aa), FASTA scores: opt: 4264, E(): 0, (87.85% identity in 742 aa overlap). Also highly similar to others e.g. Q9KZV6|PPK_STRCO from Streptomyces coelicolor (746 aa), FASTA scores: opt: 1979, E(): 2.6e-117, (59.9% identity in 701 aa overlap); Q9KD27|PPK_BACHD from Bacillus halodurans (705 aa), FASTA scores: opt: 1319, E(): 1.4e-75, (45.55% identity in 674 aa overlap); Q9PAC7|PPK_XYLFA from Xylella fastidiosa (698 aa), FASTA scores: opt: 1300, E(): 2.2e-74, (43.3% identity in 693 aa overlap); etc. Belongs to the polyphosphate kinase family. P9WHV9 No intermediary metabolism and respiration P65768 P9WHV9 GO:0006799,GO:0008976,GO:0009358,GO:0005524 2.7.4.1 P9WHV9 NC_000962.3 Mycobrowser_v5 CDS 3342165 3343118 . + 0 Rv2985 mutT1 Function unknown; hydrolytic enzyme. Possibly involved in removal of damaged nucleotide. Possible hydrolase MutT1 Rv2985, (MTCY349.02c), len: 317 aa. Possible mutT1, long MutT protein (hydrolase) (see citation below), highly similar to O33126|MLCB637.35 hypothetical 34.5 KDA protein from Mycobacterium leprae (312 aa), FASTA scores: opt: 1514, E(): 5.1e-91, (71.85% identity in 316 aa overlap); and Q9CBR8|ML1682 hypothetical protein from Mycobacterium leprae (311 aa), FASTA scores: opt: 1510, E(): 9.2e-91, (71.5% identity in 316 aa overlap). Also similar to Q50195|L222-ORF6|ML2698 hypothetical protein from Mycobacterium leprae (251 aa), FASTA scores: opt: 231, E(): 1.1e-07, (36.7% identity in 128 aa overlap). Also similar to shorter mutt proteins and related hypothetical protein e.g. Q9EUS6 hypothetical 16.6 KDA protein from Streptomyces griseus subsp. griseus (152 aa), FASTA scores: opt: 380, E(): 1.7e-17, (50.75% identity in 130 aa overlap); Q9KZV8|SCD84.10C putative mutt-like protein from Streptomyces coelicolor (142 aa), FASTA scores: opt: 376, E(): 2.9e-17, (46.1% identity in 128 aa overlap); P96590|mutt mutt protein from Bacillus subtilis (149 aa), FASTA scores: opt: 180, E(): 0.00017, (35.25% identity in 122 aa overlap); etc. Also similar to O05437 hypothetical 27.1 KDA protein from Mycobacterium tuberculosis (248 aa), FASTA scores: opt: 224, E(): 3.2e-07, (34.03% identity in 144 aa overlap). Contains PS00893 mutT domain signature. Seems to belong to the mutt/NUDIX family protein. P9WIY3 No information pathways P95110 P9WIY3 GO:0006260,GO:0016787,GO:0046872,GO:0006281 3.6.1.- P9WIY3 NC_000962.3 Mycobrowser_v5 CDS 3346147 3346848 . + 0 Rv2989 Rv2989 Involved in transcriptional mechanism. Probable transcriptional regulatory protein Rv2989, (MTV012.03), len: 233 aa. Probable transcriptional regulator (ala-rich protein), highly similar to O86533|SC1C2.33c putative transcriptional regulator from Streptomyces coelicolor (238 aa), FASTA scores: opt: 711, E(): 2.3e-38, (53.05% identity in 230 aa overlap); and similar to others e.g. Q9KND6 putative transcriptional regulator from Vibrio cholerae (244 aa), FASTA scores: opt: 232, E(): 1.2e-07, (29.75% identity in 232 aa overlap); Q9R9U0|SRPS efflux pump regulator from Pseudomonas putida (259 aa), FASTA scores: opt: 224, E(): 4.1e-07, (28.35% identity in 247 aa overlap); etc. Also similar to proteins from Mycobacterium tuberculosis e.g. O06806|Rv1773c|MTCY28.39 hypothetical 26.6 KDA protein (248 aa), FASTA scores: opt: 239, E(): 4.4e-08, (29.85% identity in 231 aa overlap); P71977|RV1719|MTCY04C12.04 hypothetical 27.9 KDA protein (259 aa), FASTA scores: opt: 215, E(): 1.6e-06, (31.85% identity in 223 aa overlap); etc. Equivalent to AAK47396 from Mycobacterium tuberculosis strain CDC1551 (267 aa) but shorter 34 aa. Contains possible helix-turn-helix motif at aa 25-46 (Score 1005, +2.61 SD). O53238 No regulatory proteins O53238 O53238 GO:0006355,GO:0006350,GO:0003677 O53238 NC_000962.3 Mycobrowser_v5 CDS 3351269 3352606 . + 0 Rv2994 Rv2994 Unknown; COULB be involved in efflux system (possibly drug). Probable conserved integral membrane protein Rv2994, (MTV012.08), len: 445 aa. Probable conserved integral membrane protein, member of major facilitator superfamily (MFS) possibly involved in transport of drug. C-terminal part highly similar to O33118|MLCB637.27c hypothetical 14.7 KDA protein (probable pseudogene product) from Mycobacterium leprae (134 aa), FASTA scores: opt: 483, E(): 2.7e-21, (60.9% identity in 138 aa overlap). Also similar to various transporters e.g. Q9I5C8|PA0811 probable MFS transporter from Pseudomonas aeruginosa (415 aa), FASTA scores: opt: 289, E(): 1.3e-09, (26.05% identity in 399 aa overlap); O30210|AF0025 cyanate transport protein from Archaeoglobus fulgidus (393 aa), FASTA scores: opt: 281, E(): 3.7e-09, (24.05% identity in 399 aa overlap); Q9RI35|SCJ12.25C putative nitrate/nitrite transporter from Streptomyces coelicolor (412 aa), FASTA scores: opt: 264, E(): 3.8e-08, (24.95% identity in 409 aa overlap); Q9A5N5|CC2412 major facilitator family transporter from Caulobacter crescentus (405 aa), FASTA scores: opt: 263, E(): 4.3e-08, (27.55% identity in 399 aa overlap); etc. First start taken; similarity to P21191|NORA_STAAU quinolone resistance protein from Staphylococcus aureus (388 aa) suggests alternative start at 7319 but then no positively charged aa before first transmembrane segment. P9WJW7 No cell wall and cell processes Q7ARU7 P9WJW7 GO:0005886,GO:0055085,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 3352458 3353468 . - 0 Rv2995c leuB Involved in leucine biosynthesis (at the third step) [catalytic activity: 3-carboxy-2-hydroxy-4-methylpentanoate + NAD(+) = 3-carboxy-4-methyl-2-oxopentanoate + NADH (the product decarboxylates to 4-methyl-2-oxopentanoate)]. Probable 3-isopropylmalate dehydrogenase LeuB (beta-IPM dehydrogenase) (IMDH) (3-IPM-DH) Rv2995c, (MTV012.09), len: 336 aa. Probable leuB, 3-isopropylmalate dehydrogenase, identical except a single bp to P94929|LEU3_MYCBO 3-isopropylmalate dehydrogenase from Mycobacterium bovis (336 aa) (see citation below), FASTA scores: opt: 2168, E(): 5.1e-132, (99.7% identity in 336 aa overlap); and equivalent to O33117|LEU3_MYCLE 3-isopropylmalate dehydrogenase from Mycobacterium leprae (336 aa), FASTA scores: opt: 1864, E(): 1.8e-112, (83.95% identity in 336 aa overlap). Also highly similar to others e.g. P94631|LEU3_CORGL from Corynebacterium glutamicum (340 aa), FASTA scores: opt: 1526, E(): 1e-90, (69.9% identity in 339 aa overlap); O86504 from Streptomyces coelicolor (347 aa), FASTA scores: opt: 1470, E(): 4.2e-87, (67.85% identity in 339 aa overlap); Q9UZ05|PAB2424 from Pyrococcus abyssi (354 aa), FASTA scores: opt: 998, E(): 1e-56, (50.0% identity in 322 aa overlap); etc. Note that also shows high similarity with many tartrate dehydrogenases. Belongs to the isocitrate and isopropylmalate dehydrogenases family. P9WKK9 No intermediary metabolism and respiration 1W0D,2G4O P95313 P9WKK9 GO:0051287,GO:0005737,GO:0009098,GO:0000287,GO:0055114,GO:0003862 1.1.1.85 P9WKK9 NC_000962.3 Mycobrowser_v5 CDS 3357602 3358567 . + 0 Rv2999 lppY Unknown Probable conserved lipoprotein LppY Rv2999, (MTV012.13), len: 321 aa. Probable lppY, conserved lipoprotein, highly similar to O07774|LPQO|Rv0604|MTCY19H5.18c putative lipoprotein from Mycobacterium tuberculosis (316 aa), FASTA scores: opt: 1153, E(): 5e-62, (53.2% identity in 312 aa overlap); and showing similarity with AAK80743|CAC2799 uncharacterized conserved protein similar to LPPY/LPQO of Mycobacterium tuberculosis from Clostridium acetobutylicum (152 aa), FASTA scores: opt: 165, E(): 0.0077, (26.08% identity in 138 aa overlap); and Q9F2T1|SCD65.01c putative lipoprotein (fragment) from Streptomyces coelicolor (146 aa), FASTA scores: opt: 126, E(): 1.6, (% identity in aa overlap). Equivalent to AAK47407 from Mycobacterium tuberculosis strain CDC1551 (329 aa) but shorter 8 aa. Contains probable N-terminal signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O53246 No cell wall and cell processes O53246 O53246 O53246 NC_000962.3 Mycobrowser_v5 CDS 3360624 3361130 . - 0 Rv3002c ilvN Involved in valine and isoleucine biosynthesis (at the first step) [catalytic activity: 2-acetolactate + CO(2) = 2-pyruvate]. Probable acetolactate synthase (small subunit) IlvN (acetohydroxy-acid synthase) (AHAS) (ALS) Rv3002c, (MT3082, MTV012.16c), len: 168 aa. Probable ilvN (alternate gene name: ilvH), acetolactate synthase, small subunit, equivalent or highly similar to others e.g. O33113|ILVH_MYCLE|MLCB637.21 from Mycobacterium leprae (169 aa), FASTA scores: opt: 843, E(): 5.1e-47, (83.5% identity in 164 aa overlap); Q59499|ILVH_MYCAV|ILVN from Mycobacterium avium (167 aa), FASTA scores: opt: 798, E(): 3.7e-44, (81.05% identity in 169 aa overlap); Q9Z566|ILVN from Streptomyces coelicolor (174 aa), FASTA scores: opt: 678, E(): 1.7e-36, (64.8% identity in 159 aa overlap); etc. Belongs to the acetolactate synthase small subunit family. P9WKJ3 No intermediary metabolism and respiration P65161 P9WKJ3 GO:0016597,GO:0009082,GO:0003984 2.2.1.6 P9WKJ3 NC_000962.3 Mycobrowser_v5 CDS 3361130 3362986 . - 0 Rv3003c ilvB1 Involved in valine and isoleucine biosynthesis (at the first step) [catalytic activity: 2-acetolactate + CO(2) = 2 pyruvate]. Acetolactate synthase (large subunit) IlvB1 (acetohydroxy-acid synthase) Rv3003c, (MT3083, MTV012.17c), len: 618 aa. ilvB1, acetolactate synthase, large subunit, equivalent or highly similar to others e.g. O33112|ILVB_MYCLE|MLCB637.20|ML1696 from Mycobacterium leprae (625 aa), FASTA scores: opt: 3653, E(): 5.4e-208, (87.1% identity in 627 aa overlap); Q59498|ILVB_MYCAV from Mycobacterium avium (621 aa), FASTA scores: opt: 3473, E(): 2.3e-197, (84.7% identity in 614 aa overlap); P42463|ILVB_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (626 aa), FASTA scores: opt: 2754, E(): 5.9e-155, (65.8% identity in 589 aa overlap); etc. Contains PS00187 Thiamine pyrophosphate enzymes signature. Cofactor: thiamine pyrophosphate, and magnesium (by similarity). Note that previously known as ilvB. P9WG41 No intermediary metabolism and respiration P0A622 P9WG41 GO:0003984,GO:0009082,GO:0000287,GO:0030976,GO:0050660 2.2.1.6 P9WG41 NC_000962.3 Mycobrowser_v5 CDS 3372545 3374620 . - 0 Rv3014c ligA This protein catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA [catalytic activity: NAD(+) + (deoxyribonucleotide)(N) + (deoxyribonucleotide)(M) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(N+M)]. DNA ligase [NAD dependent] LigA (polydeoxyribonucleotide synthase [NAD+]) Rv3014c, (MT3094, MTV012.28c), len: 691 aa. ligA (alternate gene name: lig), DNA ligase NAD-dependent (see citation below), equivalent to O33102|DNLJ_MYCLE|LIGA|LIG|ML1705|MLCB637.10 DNA ligase from Mycobacterium leprae (694 aa), FASTA scores: opt: 3844, E(): 0, (84.7% identity in 687 aa overlap). Also highly similar to many prokaryotic and eukaryotic ligases e.g. Q9Z585|LIGA|SC8D9.06 from Streptomyces coelicolor (735 aa), FASTA scores: opt: 2002, E(): 4e-113, (59.4% identity in 714 aa overlap); P49421|DNLJ_RHOMR|LIGA|LIG from Rhodothermus marinus (712 aa), FASTA scores: opt: 1835, E(): 4.6e-103, (45.55% identity in 685 aa overlap); P15042|DNLJ_ECOLI|LIGA|LIG|DNAL|PDEC|lop|B2411 from Escherichia coli strain K12 (671 aa), FASTA scores: opt: 1696, E(): 1.1e-94, (43.8% identity in 680 aa overlap); etc. Belongs to the NAD-dependent DNA ligase family. P9WNV1 No information pathways 1ZAU P63973 P9WNV1 GO:0003911,GO:0006281,GO:0006260,GO:0005622,GO:0046872,GO:0003677 6.5.1.2 P9WNV1 NC_000962.3 Mycobrowser_v5 CDS 3376490 3376852 . - 0 Rv3017c esxQ Unknown ESAT-6 like protein EsxQ (TB12.9) (ESAT-6 like protein 8) Rv3017c, (MT3097, MTV012.31c), len: 120 aa. EsxQ, ESAT-6 like protein (see citation below), possibly secreted protein, very similar to AAK47433|MT3104 putative secreted ESAT-6 like protein 9 from Mycobacterium tuberculosis strain CDC1551 (96 aa), FASTA scores: opt: 315, E(): 1.2e-14, (65.7% identity in 70 aa overlap); Rv3019c|O53266|MTV012.33c putative secreted ESAT-6 like protein 9 from Mycobacterium tuberculosis (96 aa), FASTA scores: opt: 315, E(): 1.2e-14, (65.7% identity in 70 aa overlap) and Rv0288|O53693|CFP7|MT0301|MTV035.16 10 KDA antigen CFP7 (low molecular weight protein antigen 7) (CFP-7) from Mycobacterium tuberculosis (95 aa), FASTA scores: opt: 303, E(): 7.4e-14, (66.2% identity in 68 aa overlap). An alternative start site exists at 3376801. Belongs to the ESAT6 family. Note previously known as TB12.9. P9WNJ1 No cell wall and cell processes P9WNJ1 P9WNJ1 NC_000962.3 Mycobrowser_v5 CDS 3387075 3388031 . - 0 Rv3028c fixB The electron transfer flavoprotein serves as a specific electron acceptor for other dehydrogenases. It transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Probable electron transfer flavoprotein (alpha-subunit) FixB (alpha-ETF) (electron transfer flavoprotein large subunit) (ETFLS) Rv3028c, (MTV012.43c), len: 318 aa. Probable fixB (alternate gene name: etfA), electron transfer flavoprotein (alpha subunit) for various dehydrogenases. Equivalent to O33096|ETFA_MYCLE|FIXB|ML1711|MLCB637.04 electron transfer flavoprotein from Mycobacterium leprae (318 aa), FASTA scores: opt: 1788, E(): 1.1e-87, (89.3% identity in 318 aa overlap). Also highly similar to many e.g. Q9K418|SCG22.27c from Streptomyces coelicolor (320 aa), FASTA scores: opt: 1161, E(): 1.6e-54, (59.45% identity in 323 aa overlap); AAK08137|etfa from Rhodobacter sphaeroides (308 aa), FASTA scores: opt: 792, E(): 5.1e-35, (45.95% identity in 309 aa overlap); P38974|ETFA_PARDE electron transfer flavoprotein from Paracoccus denitrificans (307 aa), FASTA scores: opt: 789, E(): 7.4e-35, (45.95% identity in 309 aa overlap); etc. Belongs to the Etf alpha-subunit / FixB family. P9WNG9 No intermediary metabolism and respiration O53275 P9WNG9 GO:0009055,GO:0022900,GO:0006810,GO:0050660 P9WNG9 NC_000962.3 Mycobrowser_v5 CDS 3389922 3391502 . + 0 Rv3031 Rv3031 Function unknown Conserved protein Rv3031, (MTV012.46), len: 526 aa. Conserved protein, equivalent to Q9CBR4|ML1714 hypothetical protein from Mycobacterium leprae (522 aa), FASTA scores: opt: 3167, E(): 4.4e-190, (86.15% identity in 526 aa overlap); and highly similar to truncated O33093|MLCB637.01c hypothetical 37.2 KDA protein (fragment) from Mycobacterium leprae (338 aa), FASTA scores: opt: 2041, E(): 5.7e-120, (84.8% identity in 342 aa overlap). Also some similarity to hypothetical proteins Q9V0M7|PAB1857 from Pyrococcus abyssi (602 aa), FASTA scores: opt: 477, E(): 3.5e-22, (31.2% identity in 556 aa overlap); and Synechocystis P74630|D90916|SLL0735 from Synechocystis sp. strain PCC 6803 (529 aa), FASTA scores: opt: 282, E(): 4.7e-10, (28.6% identity in 560 aa overlap). P9WQ27 No conserved hypotheticals O53278 P9WQ27 GO:0003824,GO:0005975 P9WQ27 NC_000962.3 Mycobrowser_v5 CDS 3399419 3400183 . - 0 Rv3039c echA17 Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. Probable enoyl-CoA hydratase EchA17 (crotonase) (unsatured acyl-CoA hydratase) (enoyl hydrase) Rv3039c, (MTV012.54c), len: 254 aa. Probable echA17, Enoyl-CoA Hydratase/Isomerase Superfamily member (crotonase). Similar to many e.g. Q9L1E6|SC3D11.16 putative enoyl-CoA hydratase from Streptomyces coelicolor (255 aa), FASTA scores: opt: 625, E(): 1.5e-30, (45.55% identity in 224 aa overlap); O07137||ECH8_MYCLE|ML2402|MLCB1306.05c probable enoyl-CoA hydratase ECHA8 from Mycobacterium leprae (257 aa), FASTA scores: opt: 448, E(): 6.4e-20, (35.3% identity in 235 aa overlap), P97087|CRT crotonase / enoyl-CoA hydratase from Clostridium thermosaccharolyticum (Thermoanaerobacterium thermosaccharolyticum) (259 aa), FASTA scores: opt: 420, E(): 3.1e-18, (31.2% identity in 234 aa overlap). Also similar to Mycobacterium tuberculosis AAK45356|O53418|Rv1070c|ECHA8|MT1100|MTV017.23c probable enoyl-CoA hydratase ECHA8 (257 aa), FASTA scores: opt: 450, E(): 4.9e-20, (36.4% identity in 226 aa overlap). Belongs to the enoyl-CoA hydratase/isomerase family. P9WNN3 No lipid metabolism O53286 P9WNN3 GO:0006631,GO:0004300 4.2.1.17 P9WNN3 NC_000962.3 Mycobrowser_v5 CDS 3400192 3401058 . - 0 Rv3040c Rv3040c Function unknown Conserved protein Rv3040c, (MTV012.55c), len: 288 aa. Conserved protein, highly similar to Q9XA40|SCH17.07c hypothetical protein from Streptomyces coelicolor (312 aa), FASTA scores: opt: 648, E(): 5.2e-34, (50.0% identity in 260 aa overlap). Also similar to Q9F7R7 predicted mutt superfamily hydrolase from uncultured proteobacterium EBAC31A08 (264 aa), FASTA scores: opt: 295, E(): 1.3e-11, (27.2% identity in 257 aa overlap); AAK24293|CC2322 hypothetical protein from Caulobacter crescentus (254 aa), blast scores: 185 (32% identity) and 131 (37% identity), etc. O53287 No conserved hypotheticals O53287 O53287 GO:0016787 O53287 NC_000962.3 Mycobrowser_v5 CDS 3405136 3406215 . + 0 Rv3044 fecB May be involved in active transport of FeIII-decitrate across the membrane (import). Probable FEIII-dicitrate-binding periplasmic lipoprotein FecB Rv3044, (MTV012.59), len: 359 aa. Probable fecB, FeIII dicitrate-binding periplasmic lipoprotein (see citation below), equivalent to Q9CBQ4|FECB|ML1729 putative FEIII-dicitrate transporter lipoprotein from Mycobacterium leprae (364 aa), FASTA scores: opt: 1816, E(): 1.1e-96, (75.65% identity in 357 aa overlap); and Q9LA57|FECB from Mycobacterium avium (364 aa), FASTA scores: opt: 1769, E(): 5.1e-94. Similar to many periplasmic FeIII-dicitrate transporters e.g. P72593|FECB|SLR1319 from Synechocystis sp. strain PCC 6803 (315 aa), FASTA scores: opt: 459, E(): 3.6e-19, (31.35% identity in 303 aa overlap); and P72611|FECB|SLR1492 from Synechocystis sp. strain PCC 6803. N-terminus longer (approximately 30 aa) to AAK47459 from Mycobacterium tuberculosis strain CDC1551 (327 aa). Has signal peptide and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site. O53291 No cell wall and cell processes O53291 O53291 GO:0005381,GO:0006827 O53291 NC_000962.3 Mycobrowser_v5 CDS 3414719 3414958 . - 0 Rv3053c nrdH Involved in electron transfer system for ribonucleotide reductase system NRDEF. Probable glutaredoxin electron transport component of NRDEF (glutaredoxin-like protein) NrdH Rv3053c, (MTCY22D7.29), len: 79 aa. Probable nrdH, glutaredoxin-like protein, equivalent to Q9CBP8|NRDH|ML1736 from Mycobacterium leprae (80 aa), FASTA scores: opt: 478, E(): 2.7e-27, (91.15% identity in 79 aa overlap), and similar to many glutaredoxin-like proteins e.g. Q9XD65|NRDH from Corynebacterium glutamicum (Brevibacterium flavum) (77 aa), FASTA scores: opt: 382, E(): 1.5e-20, (72.35% identity in 76 aa overlap); and Q56108|NRDH_SALTY from Salmonella typhimurium (81 aa), FASTA scores: opt: 243, E(): 9.9e-11, (45.85% identity in 72 aa overlap). Belongs to the glutaredoxin family. I6YB06 No information pathways P95106 I6YB06 GO:0009055,GO:0015035,GO:0045454 I6YB06 NC_000962.3 Mycobrowser_v5 CDS 3416081 3416695 . + 0 Rv3055 Rv3055 Involved in transcriptional mechanism. Possible transcriptional regulatory protein (probably TetR-family) Rv3055, (MTCY22D7.26c), len: 204 aa. Possible transcriptional regulatory protein, similar to Q9RD23|SCM1.20c putative TetR-family transcriptional regulator from Streptomyces coelicolor (234 aa), FASTA scores: opt: 471, E(): 4.6e-23, (44.9% identity in 187 aa overlap); and with low similarity to other e.g. Q9ADK8|2SCK31.12 putative TetR-family transcriptional regulator from Streptomyces coelicolor (198 aa), FASTA scores: opt: 208, 2.5e-06, (32.9% identity in 155 aa overlap); Q9ADD9|SCBAC20F6.11c putative TetR-family transcriptional from Streptomyces coelicolor (199 aa), FASTA scores: opt: 182, E(): 0.00012, (31.0% identity in 184 aa overlap). Contains potential helix-turn-helix motif from aa 48 to 69 (+3.42 SD). so may belong to the TetR/AcrR family of transcriptional regulators. P95103 No regulatory proteins P95103 P95103 GO:0006350,GO:0003700,GO:0006355 P95103 NC_000962.3 Mycobrowser_v5 CDS 3417799 3418662 . - 0 Rv3057c Rv3057c Unknown, but similar to various oxidoreductases and enzymes involved in polyketides synthesis Probable short chain alcohol dehydrogenase/reductase Rv3057c, (MTCY22D7.24), len: 287 aa. Probable oxidoreductase, probably short-chain alcohol dehydrogenase/reductase. Equivalent to Q9CBP7|ML1740 possible short chain dehydrogenases/reductase from Mycobacterium leprae (312 aa), FASTA scores: opt: 1563, E(): 6e-89, (81.8% identity in 280 aa overlap). Also similar to many oxidoreductases e.g. Q9ZBX8|SCD78.21c putative oxidoreductase from Streptomyces coelicolor (585 aa), FASTA scores: opt: 541, E(): 6.7e-26, (37.25% identity in 263 aa overlap); AAK47506|MT3170 oxidoreductase, short-chain dehydrogenase/reductase family from Mycobacterium tuberculosis strain CDC1551 (276 aa), FASTA scores: opt: 521, E(): 6.1e-25, (36.25% identity in 276 aa overlap); AAK45541|MT1283 oxidoreductase, short-chain dehydrogenase/reductase family from Mycobacterium tuberculosis strain CDC1551 (276 aa), FASTA scores: opt: 471, E(): 7.2e-22, (32.4% identity in 281 aa overlap). Also similar to O50460|Rv1245c|MTV006.17C dehydrogenase (276 aa). Contains short-chain alcohol dehydrogenase family signature (PS00061). May belong to the short-chain dehydrogenases/reductases (SDR) family. I6YB11 No intermediary metabolism and respiration P95101 I6YB11 GO:0055114,GO:0016491 1.1.-.- I6YB11 NC_000962.3 Mycobrowser_v5 CDS 3418726 3419376 . - 0 Rv3058c Rv3058c Involved in transcriptional mechanism. Possible transcriptional regulatory protein (probably TetR-family) Rv3058c, (MTCY22D7.23), len: 216 aa. Possible transcriptional regulatory protein, TetR-family, showing reasonable similarity to others e.g. AAK48337|MT3970 from Mycobacterium tuberculosis strain CDC1551 (216 aa), FASTA scores: opt: 261, E(): 2.8e-10, (31.7% identity in 221 aa overlap); Q49962|ML1070|U1756B from Mycobacterium leprae (217 aa), FASTA scores: opt: 234, E(): 1.8e-08, (27.2% identity in 195 aa overlap); Q9CDD3|ML0064 from Mycobacterium leprae (214 aa), FASTA scores: opt: 199, E(): 3.6e-06, (25.65% identity in 195 aa overlap); O66121|CPRS from Streptomyces coelicolor (215 aa), FASTA scores: opt: 183, E(): 4.2e-05, (26.0% identity in 196 aa overlap). Equivalent to AAK47476|MT3144 from Mycobacterium tuberculosis strain CDC1551 (237 aa) but N-terminus shorter 21 residues. Start was predicted by TBParse but alternatives (ATG) are possible. Could belong to the TetR/AcrR family of transcriptional regulators. P95100 No regulatory proteins P95100 P95100 GO:0006350,GO:0003700,GO:0006355 P95100 NC_000962.3 Mycobrowser_v5 CDS 3419492 3420970 . + 0 Rv3059 cyp136 Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Probable cytochrome P450 136 Cyp136 Rv3059, (MTCY22D7.22c), len: 492 aa. Probable cyp136, cytochrome P450 136, similar to other cytochrome P450-dependent oxidases e.g. Q59990|CYP120|CYP|SLR0574 putative cytochrome P450 120 from Synechocystis sp. strain PCC 6803 (444 aa), FASTA scores: opt: 579, E(): 1.5e-29, (27.3% identity in 443 aa overlap); Q64654|CYP51|CP51_RAT cytochrome P450 51 (lanosterol 14-alpha demethylase) from Rattus norvegicus (Rat) (503 aa), FASTA scores: opt: 549, E(): 1.4e-27, (26.2% identity in 458 aa overlap); Q9JIY3|CYP51 lanosterol 14-alpha-demethylase from Mus musculus (Mouse) (486 aa), FASTA scores: opt: 546, E(): 2.1e-27, (25.75% identity in 458 aa overlap). Contains cytochrome P450 cysteine heme-iron ligand signature (PS00086). Belongs to the cytochrome P450 family. P9WPM7 No intermediary metabolism and respiration P95099 P9WPM7 GO:0020037,GO:0004497,GO:0055114,GO:0009055 1.14.-.- P9WPM7 NC_000962.3 Mycobrowser_v5 CDS 3423262 3425427 . - 0 Rv3061c fadE22 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE22 Rv3061c, (MTCY22D7.20), len: 721 aa. Probable fadE22, Acyl-CoA Dehydrogenase, similar to many e.g. AAK44503|MT0284 from Mycobacterium tuberculosis strain CDC1551 (731 aa), FASTA scores: opt: 1804, E(): 1.1e-101, (43.45% identity in 743 aa overlap); AAK48037|MT3678 from Mycobacterium tuberculosis strain CDC1551 (711 aa), FASTA scores: opt: 1630, E(): 3.9e-91, (42.55% identity in 733 aa overlap); and extensive similarity in C-terminal part to many acyl-CoA dehydrogenases e.g. Q9A5G9|CC2478 from Caulobacter crescentus (407 aa), FASTA scores: opt: 767, E(): 4.8e-39, (36.7% identity in 376 aa overlap). Also similar to many hypothetical proteins. Could belong to the acyl-CoA dehydrogenases family. I6X654 No lipid metabolism P95097 I6X654 GO:0003995,GO:0055114,GO:0050660 1.3.99.- I6X654 NC_000962.3 Mycobrowser_v5 CDS 3431979 3433622 . - 0 Rv3068c pgmA This enzyme participates in both the breakdown and synthesis of glucose [catalytic activity: alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate]. Probable phosphoglucomutase PgmA (glucose phosphomutase) (PGM) Rv3068c, (MTCY22D7.13), len: 547 aa. Probable pgmA, phosphoglucomutase, highly similar to other phosphoglucomutases e.g. Q9L117|PGM from Streptomyces coelicolor (546 aa), FASTA scores: opt: 2569, E(): 2.8e-149, (71.4% identity in 545 aa overlap); Q9ABY5|CC0085 from Caulobacter crescentus (545 aa), FASTA scores: opt: 2465, E(): 6.2e-143, (70.4% identity in 541 aa overlap); P38569|PGMU_ACEXY|CELB from Acetobacter xylinum (555 aa), FASTA scores: opt: 2206, E(): 4e-127, (62.25% identity in 543 aa overlap); P74643|PGM|SLL0726 from Synechocystis sp. strain PCC 6803 (567 aa), FASTA scores: opt: 2168, E(): 8.5e-125, (60.0% identity in 550 aa overlap); P36938|PGMU_ECOLI|PGM|B0688 from Escherichia coli (546 aa), FASTA scores: opt: 2111, E(): 2.5e-121, (58.2% identity in 550 aa overlap). Also similar to other phosphomannomutases. Has phosphoglucomutase and phosphomannomutase signature (PS00710) and ATP/GTP-binding site motif A (P-loop) (PS00017). Belongs to the phosphohexose mutases family. I6Y2G3 No intermediary metabolism and respiration P95090 I6Y2G3 GO:0000287,GO:0004614,GO:0005975 5.4.2.2 I6Y2G3 NC_000962.3 Mycobrowser_v5 CDS 3435798 3436322 . - 0 Rv3072c Rv3072c Function unknown Conserved hypothetical protein Rv3072c, (MTCY22D7.09), len: 174 aa. Hypothetical protein, similar in part to O87779 hypothetical 18.1 KDA protein (fragment) from Mycobacterium paratuberculosis (166 aa), FASTA scores: opt: 238, E(): 2.5e-08, (42.6% identity in 108 aa overlap); Q9AH10 putative F420-dependent dehydrogenase from Rhodococcus erythropolis (295 aa), FASTA scores: opt: 228, E(): 1.7e-07, (34.25% identity in 111 aa overlap); P71557|Y953_MYCTU|Rv0953c|MTCY10D7.21 possible oxidoreductase from Mycobacterium tuberculosis strain H37Rv (304 aa), FASTA scores: opt: 208, E(): 3.2e-06, (38.9% identity in 108 aa overlap); etc. N-terminal region similar to several proteins from Mycobacterium tuberculosis. P95086 No conserved hypotheticals P95086 P95086 GO:0016491,GO:0055114 P95086 NC_000962.3 Mycobrowser_v5 CDS 3438050 3438973 . - 0 Rv3075c Rv3075c Function unknown Conserved protein Rv3075c, (MTCY22D7.06), len: 307 aa. Conserved protein, with some similarity to Q9I562|PA0883 probable acyl-CoA lyase beta chain from Pseudomonas aeruginosa (275 aa), FASTA scores: opt: 408, E(): 9.2e-19, (35.15% identity in 273 aa overlap); Q9S2U9|SC4G6.02 putative citrate lyase beta chain from Streptomyces coelicolor (274 aa), FASTA scores: opt: 384, E(): 3.1e-17, (34.7% identity in 265 aa overlap); O06162|cite|Rv2498c|MTCY07A7.04c from Mycobacterium tuberculosis (273 aa), FASTA scores: opt: 349, E(): 5.1e-15, (35.2% identity in 264 aa overlap); etc. Several initiation codons possible, first one chosen. I6YF40 No conserved hypotheticals P95083 I6YF40 GO:0008815,GO:0009346,GO:0046872,GO:0006725 I6YF40 NC_000962.3 Mycobrowser_v5 CDS 3439541 3441352 . + 0 Rv3077 Rv3077 Function unknown; probably involved in cellular metabolism. Possible hydrolase Rv3077, (MTCY22D7.04c), len: 603 aa. Possible hydrolase, with some similarity to variety of hydrolases (aryl- and steryl sulfatases principaly) e.g. Q45087|PEHA phosphonate monoester hydrolase from Burkholderia caryophylli (514 aa), FASTA scores: opt: 239, E(): 7.2e-07, (23.95% identity in 413 aa overlap); Q9I1E5|PA2333 probable sulfatase from Pseudomonas aeruginosa (538 aa), FASTA scores: opt: 231, E(): 2.3e-06, (28.1% identity in 516 aa overlap); P31447|YIDJ_ECOLI|B3678 putative sulfatase from Escherichia coli (497 aa), FASTA scores: opt: 222, E(): 7.4e-06, (27.7% identity in 390 aa overlap); etc. Note that previously known as atsF. Q6MX15 No intermediary metabolism and respiration Q6MX15 Q6MX15 GO:0008484,GO:0008152 3.1.-.- NC_000962.3 Mycobrowser_v5 CDS 3441770 3442597 . - 0 Rv3079c Rv3079c Function unknown Conserved protein Rv3079c, (MTCY22D7.02), len: 275 aa. Conserved protein, similar to other hypothetical mycobacterium proteins e.g. P71557|Y953_MYCTU|Rv0953c|MTCY10D7.21 possible oxidoreductase from Mycobacterium tuberculosis strain H37Rv (282 aa), FASTA scores: opt: 668, E(): 2.4e-34, (40.55% identity in 281 aa overlap); O06216|Rv2161c|MTCY270.07 from Mycobacterium tuberculosis strain H37Rv (288 aa), FASTA scores: opt: 595, E(): 8.5e-30, (40.9% identity in 274 aa overlap); O87779 from Mycobacterium paratuberculosis (166 aa), FASTA scores: opt: 464, E(): 7.2e-22, (41.55% identity in 166 aa overlap); etc. Also some similarity to other proteins e.g. Q9AH10 putative F420-dependent dehydrogenase from Rhodococcus erythropolis (295 aa), FASTA scores: opt: 401, E(): 9.6e-18, (30.2% identity in 288 aa overlap); Q9AE04|RIF17 RIF17 protein from Amycolatopsis mediterranei (356 aa), FASTA scores: opt: 298, E(): 2.8e-11, (35.0% identity in 203 aa overlap); AAK48081|MT3720 luciferase-related protein from Mycobacterium tuberculosis strain CDC1551 (395 aa), FASTA scores: opt: 223, E(): 1.4e-06, (29.4% identity in 211 aa overlap). I6XG43 No conserved hypotheticals P95079 I6XG43 GO:0016491,GO:0055114 I6XG43 NC_000962.3 Mycobrowser_v5 CDS 3442656 3445988 . - 0 Rv3080c pknK Involved in signal transduction (via phosphorylation). Involved in transcriptional regulatory mechanism and in the regulation of secondary metabolites [catalytic activity: ATP + a protein = ADP + a phosphoprotein]. Serine/threonine-protein kinase transcriptional regulatory protein PknK (protein kinase K) (STPK K) Rv3080c, (MTV013.01c-MTCY22D7.01), len: 1110 aa. pknK, serine/threonine protein kinase involved in transcriptional regulatory function (see citation below). Similar but shorter in N-terminus (approximately 300 residues) to others e.g. Q48411|ACOK transcriptional regulatory protein of aco ABCD operon from Klebsiella pneumoniae (921 aa), FASTA scores: opt: 886, E(): 7.6e-37, (27.75% identity in 829 aa overlap); Q9HX92|PA3921 probable transcriptional regulator from Pseudomonas aeruginosa (belongs to the LuxR/UhpA family of transcriptional regulators) (906 aa), FASTA scores: opt: 760, E(): 1.5e-30, (29.55% identity in 822 aa overlap); Q9I2X9|PA1760 probable transcriptional regulator from Pseudomonas aeruginosa (belongs to the LuxR/UhpA family of transcriptional regulators) (907 aa), FASTA scores: opt: 696, E(): 2.3e-27, (25.85% identity in 685 aa overlap); P06993|malt (alias BAB37683|ECS4260 and AAG58520|malt) positive regulator of MAL regulon from Escherichia coli strain O157:H7 (901 aa), FASTA scores: opt: 660, E(): 1.4e-25, (29.25% identity in 530 aa overlap); Q9KNF3|VCA0011 malt regulatory protein from Vibrio cholerae (belongs to the LuxR/UhpA family of transcriptional regulators) (921 aa), FASTA scores: opt: 626, E(): 7.2e-24, (25.8% identity in 659 aa overlap); etc. N-terminal region similar to N-terminus of serine/threonine kinases e.g. Q9KK90|PKMA serine/threonine kinase (similar to the Ser/Thr family of protein kinases) from Amycolatopsis mediterranei (589 aa), FASTA scores: opt: 545, E(): 5.7e-20, (34.45% identity in 334 aa overlap); Q9RPT5|AMK serine/threonine protein kinase homolog (similar to the Ser/Thr family of protein kinases) from Amycolatopsis mediterranei (606 aa), FASTA scores: opt: 537, E(): 1.5e-19, (35.55% identity in 346 aa overlap); Q9L0I0|PKAD protein serine/threonine kinase from Streptomyces coelicolor (599 aa), FASTA scores: opt: 520, E(): 1e-18, (36.1% identity in 324 aa overlap); etc. N-terminal part also similar to O53510|PKNL_MYCTU|Rv2176|MT2232|MTV021.09 probable serine/threonine-protein kinase from Mycobacterium tuberculosis strain H37Rv (399 aa), FASTA scores: opt: 511, E(): 2.1e-18, (35.15% identity in 313 aa overlap). Contains PS00107 Protein kinases ATP-binding region signature and PS00017 ATP/GTP-binding site motif A (P-loop). Contains Hank's kinase subdomain. First part of the protein seems belong to the Ser/Thr family of protein kinases, and second parts seems belongs to the LuxR/UhpA family of transcriptional regulators. P9WI65 No regulatory proteins P95078 P9WI65 GO:0006468,GO:0004674,GO:0005524 2.7.11.1 P9WI65 NC_000962.3 Mycobrowser_v5 CDS 3450920 3451750 . + 0 Rv3085 Rv3085 Function unknown; supposedly involved in cellular metabolism. Probable short-chain type dehydrogenase/reductase Rv3085, (MTV013.06), len: 276 aa. Probable short-chain dehydrogenase/reductase, similar to various oxidoreductases in the short chain dehydrogenases/reductases family e.g. Q9CC98|ML1094 short chain alcohol dehydrogenase from Mycobacterium leprae (277 aa), FASTA scores: opt: 1059, E(): 4.8e-56, (61.65% identity in 266 aa overlap); Q9I3H6|PA1537 probable short-chain dehydrogenase from Pseudomonas aeruginos (295 aa), FASTA scores: opt: 858, E(): 4.7e-44, (48.4% identity in 285 aa overlap); Q9CBP7|ML1740 possible short chain reductase from Mycobacterium leprae (312 aa), FASTA scores: opt: 500, E(): 1e-22, (36.6% identity in 257 aa overlap); etc. Also similar to mycobacterium proteins O50460|Rv1245c|MTV006.17c dehydrogenase similar to the short-chain dehydrogenases/reductases family (276 aa), FASTA scores: opt: 1200, E(): 1.9e-64, (65.2% identity in 273 aa overlap); and P95101|Rv3057c|MTCY22D7.24 hypothetical dehydrogenase (287 aa). Contains PS00061 Short-chain alcohol dehydrogenase family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. P9WGP9 No intermediary metabolism and respiration O53302 P9WGP9 GO:0055114,GO:0016491 1.-.-.- P9WGP9 NC_000962.3 Mycobrowser_v5 CDS 3455761 3457272 . + 0 Rv3089 fadD13 Function unknown, but involved in lipid degradation. Probable chain -fatty-acid-CoA ligase FadD13 (fatty-acyl-CoA synthetase) Rv3089, (MTV013.10), len: 503 aa. Probable fadD13, Acyl-CoA Synthetase, similar to many e.g. MTCI28.06, MTCY08D5.09, MTCY06G11.08 from Mycobacterium tuberculosis strain H37Rv; and to Q9F7P5 predicted acid--CoA ligase FADD13 from uncultured proteobacterium EBAC31A08 (504 aa), FASTA scores: opt: 1126, E(): 2.4e-62, (38.85% identity in 502 aa overlap); Q9EY88|FCS feruloyl-CoA synthetase from Amycolatopsis sp. strain HR167 (491 aa), FASTA scores: opt: 1073, E(): 4.5e-59, (38.5% identity in 504 aa overlap); BAB49118|MLR1843 probable acid-CoA ligase from Rhizobium loti (Mesorhizobium loti) (495 aa), FASTA scores: opt: 937, E(): 1.2e-50, (36.2% identity in 503 aa overlap); Q9KZC1|SC6F7.21 probable long-chain-fatty-acid-CoA ligase from Streptomyces coelicolor (511 aa), FASTA scores: opt: 899, E(): 2.8e-48, (36.1% identity in 510 aa overlap); Q9A5P7|CC2400 putative acid-CoA ligase from Caulobacter crescentus (496 aa), FASTA scores: opt: 874, E(): 9.8e-47, (35.1% identity in 507 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature and PS00061 Short-chain alcohol dehydrogenase family signature. P9WQ37 No lipid metabolism O53306 P9WQ37 GO:0008152,GO:0016874 6.2.1.- P9WQ37 NC_000962.3 Mycobrowser_v5 CDS 3459116 3460807 . + 0 Rv3091 Rv3091 Function unknown Conserved protein Rv3091, (MTCY164.02), len: 563 aa. Conserved protein, similar in part to O60859 neuropathy target esterase from Homo sapiens (Human) (1327 aa), FASTA scores: opt: 177, E(): 0.0062, (30.65% identity in 173 aa overlap); and Q9I385|PA1640 hypothetical protein from Pseudomonas aeruginosa (345 aa), FASTA scores: opt: 152, E(): 0.069, (27.8% identity in 180 aa overlap). I6YB49 No conserved hypotheticals O05770 I6YB49 GO:0006629 NC_000962.3 Mycobrowser_v5 CDS 3460814 3461734 . - 0 Rv3092c Rv3092c Unknown Probable conserved integral membrane protein Rv3092c, (MTCY164.03c), len: 306 aa. Probable conserved integral membrane protein, highly similar to Q9RUT5|DR1297 conserved hypothetical protein from Deinococcus radiodurans (311 aa), FASTA scores: opt: 941, E(): 9.8e-51, (55.65% identity in 309 aa overlap); Q9A8B8|CC1436 hypothetical protein from Caulobacter crescentus (314 aa), FASTA scores: opt: 791, E(): 1.6e-41, (46.9% identity in 305 aa overlap); and also highly similar to Q9I2N8|PA1857 hypothetical protein from Pseudomonas aeruginosa (307 aa), FASTA scores: opt: 373, E(): 8.1e-16, (40.8% identity in 321 aa overlap); BAB36119|ECS2696 putative methyl-independent mismatch repair protein from Escherichia coli strain O157:H7 (305 aa), FASTA scores: opt: 335, E(): 1.7e-13, (39.75% identity in 307 aa overlap). I6Y2I9 No cell wall and cell processes O05771 I6Y2I9 NC_000962.3 Mycobrowser_v5 CDS 3465778 3467091 . - 0 Rv3097c lipY Generates diacylglycerol and a fatty acid anion from triacylglycerol [catalytic activity: triacylglycerol + H(2)O = diacylglycerol + a fatty acid anion]. PE-PGRS family protein, triacylglycerol lipase LipY (esterase/lipase) (triglyceride lipase) (tributyrase) Rv3097c, (MTCY164.08c), len: 437 aa. LipY, triacylglycerol lipase. Belongs to the hormone-sensitive lipase family (See Deb et al., 2006) and member of the M. tuberculosis PE-family PGRS subfamily of gly-rich proteins (see citation below); N-terminal part similar to N-terminus of M. tuberculosis PE-PGRS family members e.g. Q10637|Y03A_MYCTU hypothetical glycine-rich 49.6 kDa protein (603 aa). Other relatives include MTCY1A11.25c; MTCY21B4.13c; MTCY270.06; MTCY359.33; MTC1A11.04. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). I6Y2J4 No PE/PPE P77909 I6Y2J4 GO:0004806 3.1.1.3 I6Y2J4 NC_000962.3 Mycobrowser_v5 CDS 3469301 3469783 . - 0 Rv3100c smpB Binds specifically to the SSRA RNA (TMRNA) and is required for stable association of SSRA with ribosomes. Thought to be implicated in the survival of bacterium within macrophages. Probable SSRA-binding protein SmpB Rv3100c, (MTCY164.11c), len: 160 aa. Probable smpB, small protein b related to several bacterial small protein b homologs e.g. O32881|SSRP_MYCLE|ML0671|MLCB1779.19c from Mycobacterium leprae (160 aa), FASTA scores: opt: 914, E(): 1.1e-52, (84.9% identity in 159 aa overlap); Q9L1S9|SMPB from Streptomyces coelicolor (159 aa), FASTA scores: opt: 568, E(): 3.3e-30, (55.15% identity in 145 aa overlap); O32230|SSRP_BACSU from Bacillus subtilis (156 aa), FASTA scores: opt: 511, E(): 1.7e-26, (47.05% identity in 153 aa overlap); etc. Belongs to the SSRP family. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). P9WGD3 No virulence, detoxification, adaptation P0A612 P9WGD3 GO:0005737,GO:0006412,GO:0003723 P9WGD3 NC_000962.3 Mycobrowser_v5 CDS 3469786 3470679 . - 0 Rv3101c ftsX Involved in growth (principally during log phase cells). Thought to be involved in active transport of septation component across the membrane. Responsible for the translocation of the substrate across the membrane. Is coded in an operon essential for cell division. Putative cell division protein FtsX (septation component-transport integral membrane protein ABC transporter) Rv3101c, (MTCY164.12c), len: 297 aa. Putative ftsX, cell division protein, septation component transport integral membrane protein ABC transporter (see citations below), equivalent to O32882|FTSX_MYCLE|ML0670|MLCB1779.20c cell division protein from Mycobacterium leprae (297 aa), FASTA scores: opt: 1597, E(): 9.2e-93, (80.8% identity in 297 aa overlap); and similar to others e.g. Q9L1S7|SCE59.27c from Streptomyces coelicolor (305 aa), FASTA scores: opt: 585, E(): 1.9e-29, (34.55% identity in 304 aa overlap); O34876|FTSX_BACSU from Bacillus subtilis (296 aa), FASTA scores: opt: 318, E(): 9.1e-13, (24.65% identity in 300 aa overlap); Q9K6X3|FTSX|BH3601 from Bacillus halodurans (298 aa), FASTA scores: opt: 290, E(): 5.2e-11, (22.75% identity in 299 aa overlap); etc. Belongs to the FTSX family. P9WG19 No cell wall and cell processes P96293 P9WG19 GO:0051301,GO:0016021,GO:0005886,GO:0007049 P9WG19 NC_000962.3 Mycobrowser_v5 CDS 3472768 3473904 . - 0 Rv3105c prfB Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA. Probable peptide chain release factor 2 PrfB (RF-2) Rv3105c, (MTCY164.15c), len: 378 aa. Probable prfB, peptide chain release factor 2, equivalent to O32885|RF2_MYCLE|ML0667|MLCB1779.24c from Mycobacterium leprae, FASTA scores: opt: 2197, E(): 1.8e-126, (90.05% identity in 372 aa overlap); and also similar to other peptide chain release factors e.g. Q9L1S3|PRFB from Streptomyces coelicolor (368 aa), FASTA scores: opt: 1674, E(): 1.2e-94, (69.3% identity in 365 aa overlap); O67695|RF2_AQUAE|PRFB|AQ_1840 from Aquifex aeolicus (373 aa), FASTA scores: opt: 1082, E(): 1.3e-58, (44.45% identity in 369 aa overlap); P28367|RF2_BACSU from B. subtilis (366 aa), FASTA scores: opt: 1030, E(): 1.9e-55, (44.0% identity in 359 aa overlap); etc. Also related to Q10605|MTCY373.19|RF1_MYCTU|Rv1299|MT1338 peptide chain release factor 1 (rf-1) (357 aa), FASTA scores: opt: 646, E(): 1.1e-34, (38.6% identity in 350 aa overlap). Contains prokaryotic-type class I peptide chain release factors signature (PS00745). Belongs to the prokaryotic and mitochondrial release factors family. P9WHG1 No information pathways P66026 P9WHG1 GO:0016149,GO:0006415,GO:0005737 P9WHG1 NC_000962.3 Mycobrowser_v5 CDS 3475378 3476961 . - 0 Rv3107c agpS Involved in ether lipid biosynthesis [catalytic activity: 1-acyl-glycerone 3-phosphate + a long-chain alcohol = 1-alkyl-glycerone 3-phosphate + a long-chain acid anion]. Possible alkyldihydroxyacetonephosphate synthase AgpS (alkyl-DHAP synthase) (alkylglycerone-phosphate synthase) Rv3107c, (MTCY164.17c), len: 527 aa. Possible agpS, alkyl-dihydroxyacetonephosphate synthase, similar to others and some various enzymes e.g. AAK46595|MT2311 putative alkyl-dihydroxyacetonephosphate synthase from Mycobacterium tuberculosis strain CDC1551 (529 aa), FASTA scores: opt: 1052, E(): 2.1e-58, (37.1% identity in 542 aa overlap); Q9RJ97|SCF91.28c putative flavoprotein from Streptomyces coelicolor (530 aa), FASTA scores: opt: 972, E(): 2.2e-53, (36.2% identity in 544 aa overlap); O96759|ADAS_DICDI alkyldihydroxyacetonephosphate synthase from Dictyostelium discoideum (Slime mold) (611 aa), FASTA scores: opt: 617, E(): 4.5e-31, (33.95% identity in 480 aa overlap); O97157|ADAS_TRYBB alkyldihydroxyacetonephosphate synthase from Trypanosoma brucei (613 aa), FASTA scores: opt: 567, E(): 6.2e-28, (29.15% identity in 521 aa overlap); etc. Also similar to O53525|Rv2251|MTV022.01 hypothetical 49.8 KDA protein from Mycobacterium tuberculosis strain H37Rv (475 aa), FASTA scores: opt: 1019, E(): 2.3e-56, (38.6% identity in 487 aa overlap). Belongs to the FAD-binding oxidoreductase/transferase family 4. Cofactor: FAD (by similarity). O05784 No lipid metabolism O05784 O05784 GO:0008609,GO:0016491,GO:0050660 2.5.1.26 O05784 NC_000962.3 Mycobrowser_v5 CDS 3477649 3478728 . + 0 Rv3109 moaA1 Involved in molybdenum cofactor biosynthesis; involved in the biosynthesis of molybdopterin precursor Z from guanosine. Probable molybdenum cofactor biosynthesis protein A MoaA1 Rv3109, (MTCY164.19), len: 359 aa. Probable moaA1, molybdenum cofactor biosynthesis protein, highly similar to others e.g. P39757|MOAA_BACSU|NARA|NARAB from Bacillus subtilis (341 aa), FASTA scores: opt: 810, E(): 6.2e-44, (39.75% identity in 327 aa overlap); O67929|MOAA_AQUAE|AQ_2183 from Aquifex aeolicus (320 aa), FASTA scores: opt: 794, E(): 6e-43, (40.55% identity in 323 aa overlap); Q9ZIM6|MOAA_STACA from Staphylococcus carnosus (340 aa), FASTA scores: opt: 783, E(): 3.2e-42, (38.65% identity in 326 aa overlap); etc. Also highly similar to O53143|MOAA3|MOA3_MYCTU|MT3427 molybdenum cofactor biosynthesis protein A 3 from Mycobacterium tuberculosis strain F4 (378 aa), FASTA scores: opt: 1762, E(): 4.7e-104, (74.3% identity in 350 aa overlap); and similar to O53881|MOA2_MYCTU|MOAA2|Rv0869c|MT0892|MTV043.62 molybdenum cofactor biosynthesis protein A 2 from Mycobacterium tuberculosis strain H37Rv (360 aa), FASTA scores: opt: 657, E(): 3e-34, (36.55% identity in 309 aa overlap). Belongs to the MoaA / NifB / PqqE family. Note that previously known as moaA. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WJS3 No intermediary metabolism and respiration O05786 P9WJS3 GO:0005525,GO:0006777,GO:0003824,GO:0046872,GO:0019008,GO:0051539 P9WJS3 NC_000962.3 Mycobrowser_v5 CDS 3480759 3481289 . + 0 Rv3114 Rv3114 Function unknown Conserved hypothetical protein Rv3114, (MTCY164.24), len: 176 aa. Conserved hypothetical protein, with some similarity to Q9F9W7 cytosine deaminase from Bifidobacterium longum (143 aa), FASTA scores: opt: 207, E(): 2.2e-07, (37.05% identity in 108 aa overlap); and Q9RV23|DR1207 cell cycle protein MESJ, putative/cytosine deaminase-related protein from Deinococcus radiodurans (600 aa), FASTA scores: opt: 212, E(): 3.5e-07, (33.35% identity in 177 aa overlap). Equivalent to AAK47536|MT3196 cytidine and deoxycytidylate deaminase family protein from Mycobacterium tuberculosis strain CDC1551 (187 aa) but shorter 11 aa. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O05791 No conserved hypotheticals O05791 O05791 GO:0008270,GO:0016787 O05791 NC_000962.3 Mycobrowser_v5 CDS 3482776 3483945 . + 0 Rv3116 moeB2 Possibly involved in molybdopterin metabolism (synthesis). Probable molybdenum cofactor biosynthesis protein MoeB2 (MPT-synthase sulfurylase) (molybdopterin synthase sulphurylase) Rv3116, (MTCY164.26), len: 389 aa. Probable moeB2, molybdopterin cofactor biosynthesis protein, equivalent to Q9CCG8|MOEZ|ML0817 protein probably involved in molybdopterin biosynthesis from Mycobacterium leprae (395 aa), FASTA scores: opt: 1433, E(): 8e-80, (57.8% identity in 384 aa overlap). Very similar to members of the HESA/MOEB/THIF family e.g. Q9FCL0|2SC3B6.02 putative sulfurylase from Streptomyces coelicolor (392 aa), FASTA scores: opt: 1562, E(): 1.1e-87, (58.15% identity in 380 aa overlap); Q9XC37|PDTORFF MOEB-like protein (putative sulfurylase) from Pseudomonas stutzeri (Pseudomonas perfectomarina) (391 aa), FASTA scores: opt: 1311, E(): 2.1e-72, (52.4% identity in 395 aa overlap); O54307|MPT|MOEB MPT-synthase sulfurylase from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (391 aa), FASTA scores: opt: 1238, E(): 5.7e-68, (51.4% identity in 393 aa overlap); P74344|MOEB|SLL1536 molybdopterin biosynthesis MOEB protein from Synechocystis sp. strain PCC 6803 (392 aa), FASTA scores: opt: 1212, E(): 2.2e-66, (46.5% identity in 398 aa overlap); etc. Also highly similar to O05860|MTCY07D11.20|MOEB1|Rv3206c putative molybdenum cofactor biosynthesis protein from Mycobacterium tuberculosis strain H37Rv (392 aa), FASTA scores: opt: 1445, E(): 1.5e-80, (56.25% identity in 400 aa overlap). Belongs to the HesA /MoeB/ThiF family. Note that previously known as moeB. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). L7N674 No intermediary metabolism and respiration Q7D637 L7N674 GO:0003824,GO:0008152 L7N674 NC_000962.3 Mycobrowser_v5 CDS 3484809 3485111 . + 0 Rv3118 sseC1 Thought to be involved in sulphur metabolism. Conserved hypothetical protein SseC1 Rv3118, (MTCY164.28, O05794), len: 100 aa. SseC1, conserved hypothetical protein, equivalent to Q9CBC7|ML2199 hypothetical protein from Mycobacterium leprae (100 aa), FASTA scores: opt: 545, E(): 3.1e-30, (84.0% identity in 10 aa overlap). Also similar to hypothetical proteins e.g. Q50035 from Saccharopolyspora erythraea (Streptomyces erythraeus) (101 aa), FASTA scores: opt: 345, E(): 9.7e-17, (57.15% identity in 98 aa overlap); and Q9K4H3|SCD66.02 from Streptomyces coelicolor (95 aa), FASTA scores: opt: 249, E(): 2.8e-10, (48.5% identity in 99 aa overlap). Some weak similarity with Q9ZB84|PCAG protocatechuate 3,4-dioxygenase alpha-subunit from Pseudomonas marginata (196 aa), FASTA scores: opt: 109, E(): 1.4, (31.3% identity in 83 aa overlap); and other bacterial proteins. Identical second copy present as Rv0814c|AL022004|MTV043.06c|SSEC2 from Mycobacterium tuberculosis (100 aa) (100.0% identity in 100 aa overlap). Note that previously known as sseC. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P0CG96 No intermediary metabolism and respiration Q7D986 P0CG96 NC_000962.3 Mycobrowser_v5 CDS 3485132 3485575 . + 0 Rv3119 moaE1 Possibly a molybdenum biosynthesis cofactor. Conversion of molybdopterin precursor Z into molybdopterin requires transfer of two sulfur atoms to precursor Z (to generate the dithiolene group). This is catalyzed by the converting factor composed of a small and large subunit. Probable molybdenum cofactor biosynthesis protein E MoaE1 (molybdopterin converting factor large subunit) (molybdopterin [MPT] converting factor, subunit 2) Rv3119, (MTCY164.29), len: 147 aa. Probable moaE1, molybdopterin converting factor E (molybdopterin converting factor (subunit 2)), highly similar to others e.g. O31705|MOAE from Bacillus subtilis (157 aa), FASTA scores: opt: 390, E(): 8.6e-19, (43.95% identity in 132 aa overlap); Q9K8I7|MOAE|BH3019 from Bacillus halodurans (156 aa), FASTA scores: opt: 369, E(): 2e-17, (42.4% identity in 132 aa overlap); P30749|MOAE_ECOLI|CHLA5|B0785 from Escherichia coli strain K12 (149 aa), FASTA scores: opt: 312, E(): 1.1e-13, (38.45% identity in 130 aa overlap); etc. Also highly similar (but shorter 74 aa) to O53375|GPHA|Rv3323c|MTV016.23c MOAD-MOAE fusion protein from Mycobacterium tuberculosis (221 aa), FASTA scores: opt: 733, E(): 3.9e-41, (76.2% identity in 143 aa overlap); and highly similar to O53878|MOAE2|Rv0866|MTV043.59 putative molybdopterin synthase large subunit from Mycobacterium tuberculosis (141 aa), FASTA scores: opt: 321, E(): 2.6e-14, (40.9% identity in 132 aa overlap). Note that previously known as moaE. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WJR3 No intermediary metabolism and respiration 2WP4 O05795 P9WJR3 GO:0016740,GO:0006777 2.-.-.- P9WJR3 NC_000962.3 Mycobrowser_v5 CDS 3490476 3491651 . - 0 Rv3125c PPE49 Function unknown PPE family protein PPE49 Rv3125c, (MTCY164.35c), len: 391 aa. PPE49, Member of the Mycobacterium tuberculosis PPE family, similar to other e.g. P95247|Rv2352c|MTCY98.21c (391 aa), FASTA scores: opt: 1576, E(): 3.8e-72, (62.55% identity in 398 aa overlap), MTCY98.0029c, MTCY03A2.22c, MTCY10G2.10, MTCY02B10.25c, MTCI364.08, M TCY21C12.09c, MTCY48.17. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). P9WHY5 No PE/PPE Q7D631 P9WHY5 EMB P9WHY5 NC_000962.3 Mycobrowser_v5 CDS 3496551 3497549 . + 0 Rv3131 Rv3131 Function unknown Conserved protein Rv3131, (MTCY03A2.27c), len: 332 aa. Conserved protein, similar to other hypothetical bacterial proteins e.g. O53476|Rv2032|MTV018.19 (331 aa), FASTA scores: opt: 568, E(): 2.5e-27, (36.7% identity in 321 aa overlap); O05800|Rv3127|MTCY164.37 (344 aa), FASTA scores: opt: 521, E(): 1.9e-24, (34.95% identity in 326 aa overlap); Q9RI33|SCJ12.27c from Streptomyces coelicolor (335 aa), FASTA scores: opt: 441, E(): 1.3e-19, (35.75% identity in 319 aa overlap); Q9RI44|SCJ12.14 from Streptomyces coelicolor (309 aa), FASTA scores: opt: 328, E(): 9.3e-13, (27.9% identity in 308 aa overlap); Q9CBP5|ML1751 from Mycobacterium leprae (721 aa), FASTA scores: opt: 137, E(): 0.78, (26.15% identity in 298 aa overlap); etc. Equivalent to AAK47555 from Mycobacterium tuberculosis strain CDC1551 but shorter 12 aa. Predicted possible vaccine candidate (See Zvi et al., 2008). P9WIZ7 No conserved hypotheticals P95195 P9WIZ7 GO:0016491,GO:0055114 P9WIZ7 NC_000962.3 Mycobrowser_v5 CDS 3499943 3500749 . - 0 Rv3134c Rv3134c Function unknown. Could play a role in the adaptation to hypoxia, participating in the phosphorelay in the two component regulatory system DEVR|Rv3133c/DEVS|Rv3132c. Universal stress protein family protein Rv3134c, (MTCY03A2.240, len: 268 aa. Universal stress protein family protein. Ala-, Val- rich (see citations below), related to other hypothetical Mycobacterium tuberculosis proteins e.g. O53474|Rv2028c|MTV018.15c (279 aa), FASTA scores: opt: 562, E(): 3.2e-28, (40.65% identity in 273 aa overlap); O06188|Rv2624c|MTCY01A10.08 (272 aa), FASTA scores: opt: 458, E(): 1.1e-21, (36.55% identity in 271 aa overlap); O53472|R2026c|MTV018.13c (294 aa), FASTA scores: opt: 232, E(): 1.9e-07, (30.45% identity in 276 aa overlap); etc. Shares some similarity with other hypothetical proteins from Streptomyces coelicolor e.g. Q9RIZ8|SCJ1.16c (294 aa), FASTA scores: opt: 207, E(): 6.9e-06, (28.9% identity in 263 aa overlap); Q9K4L5|SC5F8.09 putative stress-inducible protein (312 aa), FASTA scores: opt: 204, E(): 1.1e-05, (28.4% identity in 271 aa overlap); etc. Equivalent to AAK47558|MT3220 Universal stress protein family from Mycobacterium tuberculosis strain CDC1551 (268 aa). Rv3134c seems cotranscribed with devR-devS (see Sherman et al., 2001). P9WFD3 No virulence, detoxification, adaptation P95192 P9WFD3 GO:0006950 P9WFD3 NC_000962.3 Mycobrowser_v5 CDS 3505363 3506769 . + 0 Rv3139 fadE24 Function unknown, but involved in lipid degradation. Probable acyl-CoA dehydrogenase FadE24 Rv3139, (MTCY03A2.19c), len: 468 aa. Probable fadE24, acyl-CoA dehydrogenase (1.3.99.-), equivalent to O32890|MLCB1779.30|FADE24|ML0661 putative acyl-CoA dehydrogenase from Mycobacterium leprae (465 aa), FASTA scores: opt: 2587, E(): 4e-153, (83.6% identity in 464 aa overlap). Similar to other e.g. Q9HUH0|PA4995 from Pseudomonas aeruginosa (429 aa), FASTA scores: opt: 1139, E(): 2.8e-63, (45.3% identity in 426 aa overlap); Q9K6D0|MMGC|BH3799 from Bacillus halodurans (379 aa), FASTA scores: opt: 603, E(): 4.7e-30, (30.3% identity in 366 aa overlap); Q9K6D1|ACDA|BH3798 from Bacillus halodurans (380 aa), FASTA scores: opt: 601, E(): 6.3e-30, (32.25% identity in 363 aa overlap); etc. Contains acyl-CoA dehydrogenases signature 2 (PS00073) near C-terminus. Belongs to the acyl-CoA dehydrogenases family. P95187 No lipid metabolism P95187 P95187 GO:0003995,GO:0055114,GO:0050660 1.3.99.- INH P95187 NC_000962.3 Mycobrowser_v5 CDS 3508095 3509066 . + 0 Rv3141 fadB4 Involved in lipid degradation [catalytic activity: NADPH + quinone = NADP(+) + semiquinone]. Probable NADPH quinone oxidoreductase FadB4 (NADPH:quinone reductase) (zeta-crystallin) Rv3141, (MTCY03A2.17c), len: 323 aa. Probable fadB4, quinone oxidoreductase, showing strong similarity to variety of quinone oxidoreductases and domains in polyketide and fatty acid synthases e.g. Q9HTV6|PA5234 probable oxidoreductase from Pseudomonas aeruginosa (325 aa), FASTA scores: opt: 737, E(): 1.4e-35, (39.65% identity in 328 aa overlap); Q9RYQ7|DRA0251 putative NADPH quinone oxidoreductase from Deinococcus radiodurans (336 aa), FASTA scores: opt: 688, E(): 1e-32, (40.6% identity in 325 aa overlap); Q9RVG8|DR1061 putative NADPH quinone oxidoreductase from Deinococcus radiodurans (388 aa), FASTA scores: opt: 559, E(): 3.3e-25, (36.3% identity in 325 aa overlap); BAB49685|MLL2594 probable quinone oxidoreductase from Rhizobium loti (Mesorhizobium loti) (326 aa), FASTA scores: opt: 519, E(): 5.9e-23, (34.25% identity in 330 aa overlap); Q9LXZ4|T5P19_110 quinone reductase-like protein from Arabidopsis thaliana (348 aa), FASTA scores: opt: 517, E(): 8.1e-23, (33.55% identity in 322 aa overlap); etc. Also similar to Q9AA38|CC0770 zinc-containing alcohol dehydrogenase from Caulobacter crescentus (325 aa), FASTA scores: opt: 673, E(): 7.2e-32, (40.2% identity in 326 aa overlap); and Q9ABX4|CC0096 zinc-containing alcohol dehydrogenase from Caulobacter crescentus (332 aa), FASTA scores: opt: 623, E(): 5.7e-29, (40.7% identity in 334 aa overlap). Also resembles Mycobacterium tuberculosis proteins P96826|Rv0149|MTCI5_23, MTCY13D12.11, MTCY24G1.03, MTCY19H9.01. Belongs to the zinc-containing alcohol dehydrogenase family, quinone oxidoreductase subfamily. Thought to be differentially expressed within host cells (see Triccas et al., 1999). P95185 No lipid metabolism P95185 P95185 GO:0055114,GO:0008270,GO:0003960 1.6.5.5 P95185 NC_000962.3 Mycobrowser_v5 CDS 3512628 3513338 . + 0 Rv3147 nuoC Involved in aerobic|anaerobic respiration [catalytic activity: NADH + ubiquinone = NAD(+) + ubiquinol]. Probable NADH dehydrogenase I (chain C) NuoC (NADH-ubiquinone oxidoreductase chain C) Rv3147, (MTCY03A2.11c), len: 236 aa. Probable nuoC, NADH dehydrogenase, chain C, similar to others e.g. Q9XAQ6|NUOC from Streptomyces coelicolor (251 aa), FASTA scores: opt: 1113, E(): 2.6e-64, (67.35% identity in 236 aa overlap); Q9A6X2|CC1954 from Caulobacter crescentus (197 aa), FASTA scores: opt: 351, E(): 1.6e-15, (41.65% identity in 132 aa overlap); BAB48757|MLL1369 from Rhizobium loti (Mesorhizobium loti) (201 aa), FASTA scores: opt: 347, E(): 3e-15, (42.4% identity in 132 aa overlap); etc. Also similar to Q9UUU0|NUGM NUGM protein precursor from Yarrowia lipolytica (Candida lipolytica) (281 aa), FASTA scores: opt: 356, E(): 1.1e-15, (34.55% identity in 162 aa overlap). Also similar to MTCY251.05, FASTA score: E():4.9e-05. Equivalent to AAK47574 from Mycobacterium tuberculosis strain CDC1551 but longer 26 aa. Belongs to the complex I 30 KDA subunit family. P9WJH3 No intermediary metabolism and respiration P65571 P9WJH3 GO:0055114,GO:0019684,GO:0005886,GO:0048038,GO:0006810,GO:0008137 1.6.99.5 P9WJH3 NC_000962.3 Mycobrowser_v5 CDS 3515412 3516749 . + 0 Rv3150 nuoF Involved in aerobic|anaerobic respiration [catalytic activity: NADH + ubiquinone = NAD(+) + ubiquinol]. Probable NADH dehydrogenase I (chain F) NuoF (NADH-ubiquinone oxidoreductase chain F) Rv3150, (MTCY03A2.08c), len: 445 aa. Probable nuoF, NADH dehydrogenase, chain F, similar to others e.g. Q9XAQ9|NUOF_STRCO from Streptomyces coelicolor (449 aa), FASTA scores: opt: 2314, E(): 3.5e-139, (76.25% identity in 434 aa overlap); NUF2_RHIME from Rhizobium meliloti (421 aa), FASTA scores: opt: 1545, E(): 1.8e-90, (53.1% identity in 424 aa overlap); Q9RU92|DR1500 from Deinococcus radiodurans (444 aa), FASTA scores: opt: 1445, E(): 4.1e-84, (52.9% identity in 427 aa overlap); etc. Contains respiratory-chain NADH dehydrogenase 51 Kd subunit signature 2 (PS00645). Belongs to the complex I 51 KDA subunit family. Cofactor: FMN and one 4FE-4S cluster (probable). P9WIV7 No intermediary metabolism and respiration P65567 P9WIV7 GO:0010181,GO:0051287,GO:0008137,GO:0046872,GO:0055114,GO:0048038,GO:0051539 1.6.99.5 P9WIV7 NC_000962.3 Mycobrowser_v5 CDS 3519282 3520514 . + 0 Rv3152 nuoH Involved in aerobic|anaerobic respiration [catalytic activity: NADH + ubiquinone = NAD(+) + ubiquinol]. Probable NADH dehydrogenase I (chain H) NuoH (NADH-ubiquinone oxidoreductase chain H) Rv3152, (MTCY03A2.06c), len: 410 aa. Probable nuoH, integral membrane NADH dehydrogenase I, chain H, similar to others e.g. Q9XAR1 Q9XAR1|NUOH from Streptomyces coelicolor (467 aa), FASTA scores: opt: 1630, E(): 3.4e-90, (58.35% identity in 413 aa overlap); Q9RU94|DR1498 from Deinococcus radiodurans (397 aa), FASTA scores: opt: 1081, E(): 2e-57, (45.5% identity in 391 aa overlap); Q9ZCF7|NUOH_RICPR|RP796 from Rickettsia prowazekii (339 aa), FASTA scores: opt: 976, E(): 3.4e-51, (46.2% identity in 329 aa overlap); etc. Contains respiratory-chain NADH dehydrogenase subunit 1 signature 2 (PS00668). Some similarity to MTCY251.02 (FASTA score: E(): 1.2e-07). Belongs to the complex I subunit 1 family. P9WIX1 No intermediary metabolism and respiration P65561 P9WIX1 GO:0016021,GO:0055114,GO:0005886,GO:0048038,GO:0050136 1.6.99.5 NC_000962.3 Mycobrowser_v5 CDS 3521139 3521927 . + 0 Rv3154 nuoJ Involved in aerobic|anaerobic respiration [catalytic activity: NADH + ubiquinone = NAD(+) + ubiquinol]. Probable NADH dehydrogenase I (chain J) NuoJ (NADH-ubiquinone oxidoreductase chain J) Rv3154, (MTCY03A2.04c), len: 262 aa. Probable nuoJ, transmembrane NADH dehydrogenase I, chain J, similar to others e.g. Q9XAR3|NUOJ from Streptomyces coelicolor (285 aa), FASTA scores: opt: 991, E(): 3.2e-52, (63.7% identity in 243 aa overlap); Q9JX90|NUOJ|NMA0006 from Neisseria meningitidis (serogroup A) (223 aa), FASTA scores: opt: 329, E(): 9.6e-13, (34.85% identity in 175 aa overlap); Q9K1B2|NMB0253 from Neisseria meningitidis (serogroup B) (223 aa), FASTA scores: opt: 326, E(): 1.5e-12, (34.85% identity in 175 aa overlap); etc. But also similarity with Q00243|NU6C_PLEBO|NDH6 NADH-plastoquinone oxidoreductase chain 6 homolog (catalytic activity: NADH + plastoquinone = NAD(+) + plastoquinol) from Plectonema boryanum (199 aa), FASTA scores: opt: 287, E(): 2.8e-10, (34.35% identity in 195 aa overlap). Similar to polypeptide 6 of the NADH-ubiquinol oxidoreductase of chloroplasts or mitochondria. P95172 No intermediary metabolism and respiration P95172 P95172 GO:0055114,GO:0008137 1.6.5.3 P95172 NC_000962.3 Mycobrowser_v5 CDS 3521924 3522223 . + 0 Rv3155 nuoK Involved in aerobic|anaerobic respiration [catalytic activity: NADH + ubiquinone = NAD(+) + ubiquinol]. Probable NADH dehydrogenase I (chain K) NuoK (NADH-ubiquinone oxidoreductase chain K) Rv3155, (MTCY03A2.03c), len: 99 aa. Probable nuoK, integral membrane NADH dehydrogenase I, chain K, similar to others e.g. Q9XAR4|NUOK from Streptomyces coelicolor (99 aa), FASTA scores: opt: 509, E(): 2.7e-31, (78.55% identity in 98 aa overlap); Q56226|NQOB_THETH|NQO11 from Thermus aquaticus (subsp. thermophilus) (95 aa), blast scores: initn: 298, init1: 180, bits: 85.7, FASTA scores: opt: 313, E(): 9.4e-17, (53.7% identity in 95 aa overlap); Q9RU97|DR1495 from Deinococcus radiodurans (103 aa), FASTA scores: opt: 309, E(): 2e-16, (52.0% identity in 100 aa overlap); etc. But also similarity with NADH-plastoquinone oxidoreductases chain 4L e.g. Q9MUL4|NULC_MESVI|NDHE from Mesostigma viride (catalytic activity: NADH + plastoquinone = NAD(+) + plastoquinol) (101 aa), FASTA scores: opt: 280, E(): 2.8e-14, (40.6% identity in 101 aa overlap); and P06261|NULC_TOBAC|NDHE|NDH4L from Nicotiana tabacum (Common tobacco) (101 aa), FASTA scores: opt: 259, E(): 1e-12, (43.0% identity in 93 aa overlap). Similar to polypeptide 4L of the NADH-ubiquinol oxidoreductase of chloroplasts or mitochondria. P9WIX3 No intermediary metabolism and respiration P65565 P9WIX3 GO:0050136,GO:0016021,GO:0019684,GO:0005886,GO:0048038,GO:0006810,GO:0042773 1.6.99.5 P9WIX3 NC_000962.3 Mycobrowser_v5 CDS 3522234 3524135 . + 0 Rv3156 nuoL Involved in aerobic|anaerobic respiration [catalytic activity: NADH + ubiquinone = NAD(+) + ubiquinol]. Probable NADH dehydrogenase I (chain L) NuoL (NADH-ubiquinone oxidoreductase chain L) Rv3156, (MTCY03A2.02c), len: 633 aa. Probable nuoL, integral membrane NADH dehydrogenase I, chain L, similar to others e.g. Q9XAR5|NUOL_STRCO from Streptomyces coelicolor (654 aa), FASTA scores: opt: 2074, E(): 1.1e-111, (61.1% identity in 648 aa overlap); Q56227|NQOC_THETH|NQO12 from Thermus aquaticus (subsp. thermophilus) (606 aa), FASTA scores: opt: 1420, E(): 3.8e-74, (43.35% identity in 630 aa overlap); Q9ZJV6|NUOL|JHP1192 from Helicobacter pylori J99 (Campylobacter pylori J99) (612 aa), FASTA scores: opt: 1279, E(): 4.7e-66, (41.65% identity in 516 aa overlap); etc. Also similar to MTCY251.04 (FASTA score: E(): 1.3e-11) and MTCY03A2.01c (FASTA score: E(): 2.3e-10). Similar to polypeptide 5 of the NADH-ubiquinol oxidoreductase of chloroplasts or mitochondrial. P9WIW1 No intermediary metabolism and respiration O86350 P9WIW1 GO:0008137,GO:0016021,GO:0005886,GO:0048038,GO:0042773 1.6.99.5 P9WIW1 NC_000962.3 Mycobrowser_v5 CDS 3525790 3527385 . + 0 Rv3158 nuoN Involved in aerobic|anaerobic respiration [catalytic activity: NADH + ubiquinone = NAD(+) + ubiquinol]. Probable NADH dehydrogenase I (chain N) NuoN (NADH-ubiquinone oxidoreductase chain N) Rv3158, (MTV014.02c), len: 531 aa. Probable nuoN, integral membrane NADH dehydrogenase I, chain N, similar to others e.g. Q9XAR7|SC10A7.08c from Streptomyces coelicolor (552 aa), FASTA scores: opt: 1493, E(): 1.1e-81, (56.7% identity in 543 aa overlap); Q9PGI2|XF0318 from Xylella fastidiosa (485 aa), FASTA scores: opt: 942, E(): 7.4e-49, (39.6% identity in 379 aa overlap); CAB51628|NUON2 from Rhizobium meliloti (Sinorhizobium meliloti) (479 aa), FASTA scores: opt: 934, E(): 2.2e-48, (35.5% identity in 479 aa overlap); etc. But also similarity with NADH-plastoquinone oxidoreductases chain 4L (catalytic activity: NADH + plastoquinone = NAD(+) + plastoquinol) e.g. P29801|NU2C_SYNP7|NDHB from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (521 aa), FASTA scores: opt: 921, E(): 1.4e-47, (40.25% identity in 395 aa overlap). Belongs to the complex I subunit 2 family. P9WIW9 No intermediary metabolism and respiration P0A5M0 P9WIW9 GO:0008137,GO:0016021,GO:0019684,GO:0005886,GO:0048038,GO:0006810,GO:0042773 1.6.99.5 NC_000962.3 Mycobrowser_v5 CDS 3535431 3536057 . - 0 Rv3167c Rv3167c Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably TetR-family) Rv3167c, (MTV014.11c), len: 208 aa. Probable transcriptional regulator, TetR family, similar to several transcriptional regulators e.g. Q9L2A4|SC8F4.22c (TetR/AcrR family) from Streptomyces coelicolor (234 aa), FASTA scores: opt: 317, E(): 7.5e-13, (33.35% identity in 210 aa overlap); Q9RK47|SCF12.11 (TetR/AcrR family) from Streptomyces coelicolor (206 aa), FASTA scores: opt: 293, E(): 2.1e-11, (32.65% identity in 199 aa overlap); Q54288 regulator of antibiotic transport complexes (TetR/AcrR family) (204 aa), FASTA scores: opt: 260, E(): 2.4e-09, (30.75% identity in 205 aa overlap); etc. Equivalent to AAK47595 from Mycobacterium tuberculosis strain CDC1551 but shorter 21 aa. Contains probable helix-turn-helix motif from aa 42 to 63 (Score 1727, +5.07 SD). May belong to the TetR/AcrR family of transcriptional regulators. O53317 No regulatory proteins O53317 O53317 GO:0006350,GO:0003700,GO:0006355 O53317 NC_000962.3 Mycobrowser_v5 CDS 3538505 3539851 . + 0 Rv3170 aofH Possibly catalyzes the oxidative deamination: oxidize on primary amines, and perhaps on secondary and tertiary amines [catalytic activity: RCH(2)NH(2) + H(2)O + O(2) = RCHO + NH(3) + H(2)O(2)]. Must have important function in metabolism. Supposedly involved in stationary-phase survival. Probable flavin-containing monoamine oxidase AofH (amine oxidase) (MAO) Rv3170, (MT3259, MTV014.14), len: 448 aa. Probable aofH, flavin-containing (mono)amine oxidase, equivalent to a predicted homologous protein from Mycobacterium smegmatis (see citation below), and similar to many eukaryotic monoamine oxidases e.g. P49253|AOF_ONCMY from Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri) (522 aa), FASTA scores: opt: 869, E(): 5.3e-44, (37.7% identity in 448 aa overlap); P21396|AOFA_RAT|MAOA from Rattus norvegicus (Rat) (526 aa), FASTA scores: opt: 839, E(): 3.2e-42, (37.45% identity in 446 aa overlap); Q99NA8|MAO-a from Cavia porcellus (Guinea pig) (506 aa), FASTA scores: opt: 836, E(): 4.6e-42, (37.0% identity in 446 aa overlap); P21398|AOFA_BOVIN from Bos taurus (Bovine) (527 aa), FASTA scores: opt: 806, E(): 2.8e-40, (37.0% identity in 446 aa overlap); P21397|AOFA_HUMAN (527 aa), FASTA scores: opt: 801, E(): 5.6e-40, (37.2% identity in 446 aa overlap); etc. Alternative start possible at position 3538487. Belongs to the flavin monoamine oxidase family. Cofactor: FAD (potential). P9WQ15 No intermediary metabolism and respiration P63533 P9WQ15 GO:0016491,GO:0055114 1.4.3.- P9WQ15 NC_000962.3 Mycobrowser_v5 CDS 3539846 3540745 . - 0 Rv3171c hpx Supposedly involved in detoxification reactions. Possible non-heme haloperoxidase Hpx Rv3171c, (MTV014.15c), len: 299 aa. Possible hpx, non-heme haloperoxidase, similar to other hydrolases (principaly epoxide hydrolases) and non-heme chloroperoxidases e.g. Q9RKB6|SCE87.22c putative hydrolase from Streptomyces coelicolor (314 aa), FASTA scores: opt: 431, E(): 6e-20, (38.05% identity in 297 aa overlap); Q9HZ14|PA3226 probable hydrolase (similar to alpha/beta hydrolase fold) from Pseudomonas aeruginosa (275 aa), FASTA scores: opt: 236, E(): 1e-07, (29.6% identity in 277 aa overlap); Q9DBL9|1300003 D03RIK protein similar to alpha/beta hydrolase fold from Mus musculus (Mouse) (351 aa), FASTA scores: opt: 223, E(): 8.3e-07, (24.35% identity in 304 aa overlap); AAK46260|MT1988 epoxide hydrolase from Mycobacterium tuberculosis strain CDC1551 (356 aa), FASTA scores: opt: 223, E(): 8.4e-07, (40.7% identity in 113 aa overlap); P49323|PRXC_STRLI|CPO|CPOL non-heme chloroperoxidase (chloride peroxidase) from Streptomyces lividans (275 aa), FASTA scores: opt: 220, E(): 1e-06, (29.5% identity in 305 aa overlap); etc. Equivalent to AAK47599 Hydrolase, alpha/beta hydrolase family from Mycobacterium tuberculosis strain CDC1551 but shorter 24 aa. Start chosen by similarity, alternative with good RBS possible. O53321 No virulence, detoxification, adaptation O53321 O53321 GO:0004601,GO:0055114 1.11.1.- O53321 NC_000962.3 Mycobrowser_v5 CDS 3542860 3544347 . + 0 Rv3175 Rv3175 Function unknown; involved in cellular metabolism. Possible amidase (aminohydrolase) Rv3175, (MTV014.19), len: 495 aa. Possible amidase , similar to others e.g. Q9F6D0|ZHUL enantiomer selective amidase from Streptomyces sp. R1128 (507 aa), FASTA scores: opt: 1328 ,E(): 7.5e-69, (44.5% identity in 492 aa overlap); BAB51815|MLR5350 probable amidase from Rhizobium loti (Mesorhizobium loti) (457 aa), FASTA scores: opt: 7487, E(): 1.3e-35, (35.9% identity in 482 aa overlap); O28325|YJ54_ARCFU|AF1954 putative amidase from Archaeoglobus fulgidus (453 aa), FASTA scores: opt: 532, E(): 3.2e-23, (32.05% identity in 471 aa overlap); etc. But also similar to glutamyl-tRNA amidotransferases who belong to amidase family e.g. Q9RTA9|DR1856 glutamyl-tRNA(GLN) amidotransferase, subunit A from Deinococcus radiodurans (482 aa), FASTA scores: opt: 560, E(): 8.2e-25, (30.6% identity in 513 aa overlap); Q9LCX3|GATA GLU/asp-tRNA amidotransferase subunit A from Thermus aquaticus (subsp. thermophilus) (471 aa), FASTA scores: opt: 558, E(): 1.1e-24, (30.85% identity in 486 aa overlap); Q49091|GATA_MORCA glutamyl-tRNA(GLN) amidotransferase subunit A from Moraxella catarrhalis (492 aa), FASTA scores: opt: 526, E(): 7.5e-23, (30.45% identity in 473 aa overlap); etc. Seems to belong to the amidase family. Contains PS00017 ATP/GTP-binding site motif A (P-loop). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53325 No intermediary metabolism and respiration O53325 O53325 GO:0016787,GO:0016884 3.5.1.- O53325 NC_000962.3 Mycobrowser_v5 CDS 3544344 3545300 . - 0 Rv3176c mesT Biotransformation enzyme that acts on a variety of epoxides and arene oxides. Catalyzes the hydrolysis of arene and epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water [catalytic activity: an epoxide + H(2)O = a glycol]. Probable epoxide hydrolase MesT (epoxide hydratase) (arene-oxide hydratase) Rv3176c, (MTV014.20c), len: 318 aa. Probable mesT, epoxide hydrolase, similar to others e.g. O15007|PEG1|MEST|Q92571|O14973 MEST protein (mesoderm specific transcript (mouse) homolog) (similar to alpha/beta hydrolase fold) from Homo sapiens (Human) (335 aa), FASTA scores: opt: 348, E(): 6e-15, (32.15% identity in 280 aa overlap); AAH06639|Q07646 MEST protein from Mus musculus (Mouse) (335 aa), FASTA scores: opt: 342, E(): 1.4e-14, (31.45% identity in 280 aa overlap); Q9I8E7|MEST epoxide hydrolase from Fugu rubripes (Japanese pufferfish) (Takifugu rubripes) (326 aa), FASTA scores: opt: 322, E(): 2.7e-13, (29.55% identity in 301 aa overlap); Q9PUC9|PEG1|MEST epoxide hydrolase from Brachydanio rerio (Zebrafish) (Zebra danio) (344 aa), FASTA scores: opt: 322, E(): 2.8e-13, (32.35% identity in 207 aa overlap); Q9HYH6|PA3429 probable epoxide hydrolase from Pseudomonas aeruginosa (298 aa), FASTA scores: opt: 258, E(): 3e-09, (29.85% identity in 288 aa overlap); O31243|ECHA epoxide hydrolase from Agrobacterium radiobacter (294 aa), FASTA scores: opt: 202, E(): 1.1e-05, (27.0% identity in 278 aa overlap); etc. Also similar to Q50599|Rv1834|MT1882|MTCY1A11.09c hypothetical 31.7 KDA protein from Mycobacterium tuberculosis (288 aa), FASTA scores: opt: 294, E(): 1.5e-11, (29.95% identity in 287 aa overlap). Equivalent to AAK47604 from Mycobacterium tuberculosis strain CDC1551 (339 aa) but shorter 21 aa. Similar to alpha/beta hydrolase fold. May belong to peptidase family S33. Note that previously known as lipS. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Q6MX03 No virulence, detoxification, adaptation Q6MX03 GO:0016787 3.3.2.3 Q6MX03 NC_000962.3 Mycobrowser_v5 CDS 3557311 3558345 . - 0 Rv3191c Rv3191c Involved in the transposition of an insertion sequence. Probable transposase Rv3191c, (MTV014.35c), len: 344 aa. Probable transposase, similar to many especially Q9K2N8 putative transposase from Pseudomonas aeruginosa (338 aa), FASTA scores: opt: 837, E(): 1.3e-43, (42.55% identity in 336 aa overlap); Q9RBF4 insertion sequence IS1088 from Alcaligenes eutrophus (Ralstonia eutropha) (342 aa), FASTA scores: opt: 823, E(): 9.2e-43, (43.05% identity in 337 aa overlap); and Q51379 putative transposase from Pseudomonas alcaligenes (338 aa), FASTA scores: opt: 818, E(): 1.8e-42, (42.35% identity in 333 aa overlap). Contains probable helix-turn-helix motif from aa 25 to 46 (Score 1968, +5.89 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). O53337 No insertion seqs and phages O53337 O53337 GO:0015074,GO:0003677 O53337 NC_000962.3 Mycobrowser_v5 CDS 3560194 3563172 . - 0 Rv3193c Rv3193c Unknown Probable conserved transmembrane protein Rv3193c, (MTV014.37c), len: 992 aa. Probable conserved transmembrane protein, with hydrophobic N-terminal domain (~1-340 aa), highly similar to Q9CCM6|ML0644 putative integral membrane protein from Mycobacterium leprae (983 aa), FASTA scores: opt: 5421, E(): 0, (86.15% identity in 989 aa overlap); and O53609|Rv0064|MTV030.07 putative membrane protein from Mycobacterium tuberculosis strain H37Rv (979 aa), FASTA scores: opt: 3204, E(): 2.1e-142, (50.25% identity in 985 aa overlap). C-terminal part (709-990 aa) highly similar to O32904|MLCB1779.46 hypothetical 29.1 KDA protein from Mycobacterium leprae (277 aa), FASTA scores: opt: 1521, E(): 3.4e-64, (82.6% identity in 282 aa overlap). Also some similarity to hypothetical proteins generally transmembrane e.g. Q9FCI4|2SC3B6.28 from Streptomyces coelicolor (815 aa), FASTA scores: opt: 951, E(): 3.4e-37, (39.2% identity in 826 aa overlap); P72637|SLL1060 from Synechocystis sp. strain PCC 6803 (1032 aa), FASTA scores: opt: 938, E(): 1.6e-36, (29.95% identity in 855 aa overlap); O28851|AF1421 from Archaeoglobus fulgidus (880 aa), FASTA scores: opt: 526, E(): 2.6e-17, (28.05% identity in 970 aa overlap); etc. P9WFL3 No cell wall and cell processes O53339 P9WFL3 GO:0005886,GO:0016021 NC_000962.3 Mycobrowser_v5 CDS 3567024 3568367 . + 0 Rv3197 Rv3197 Thought to be involved in active transport across the membrane. Responsible for energy coupling to the transport system. Probable conserved ATP-binding protein ABC transporter Rv3197, (MTV014.41), len: 447 aa. Probable conserved ATP-binding protein ABC transporter, highly similar to Mycobacterium leprae proteins: Q9CCM8|ML0640 hypothetical protein (473 aa), FASTA scores: opt: 2512, E(): 2.1e-140, (83.0% identity in 447 aa overlap). Interestingly, the N-terminal half (1-219 aa) corresponds to Q49747|ABC1|B1937_C3_233 ABC1 protein from Mycobacterium leprae (267 aa), FASTA scores: opt: 1276, E(): 6.3e-68, (88.6% identity in 219 aa overlap); and the C-terminal half (239-447 aa) corresponds to Q49745|B1937_C2_179 hypothetical 23.1 KDA protein (206 aa), FASTA scores: opt: 1138, E(): 6.5e-60, (77.05% identity in 209 aa overlap); two adjacent orfs from Mycobacterium leprae. Also highly similar to other proteins (generally ABC transporters) e.g. Q9FCJ6|2SC3B6.16c hypothetical 51.3 KDA protein from Streptomyces coelicolor (469 aa), FASTA scores: opt: 1340, E(): 1.8e-71, (45.9% identity in 449 aa overlap); O65576|ABC1AT ABC1 protein (alias Q9SBB2|T15B16.14|AT4G01660 putative ABC transporter) from Arabidopsis thaliana (Mouse-ear cress) (623 aa), FASTA scores: opt: 543, E(): 1.7e-24, (28.4% identity in 405 aa overlap); O27682|MTH1645 ABC transporter from Methanobacterium thermoautotrophicum (623 aa), FASTA scores: opt: 497, E(): 7.8e-22, (33.0% identity in 309 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-binding transport protein family (ABC transporters). O53343 No cell wall and cell processes O53343 O53343 GO:0005524 O53343 NC_000962.3 Mycobrowser_v5 CDS 3568401 3568679 . - 0 Rv3197A whiB7 Involved in transcriptional mechanism. Probable transcriptional regulatory protein WhiB-like WhiB7 Rv3197A, len: 92 aa. Probable whiB7 (alternate gene name: whmC), WhiB-like regulatory protein (see citation below), similar to WhiB paralogue of Streptomyces coelicolor, wblE gene product (85 aa). Equivalent to Q49765|WHIB7|ML0639|B1937_F2_68 putative transcriptional regulator WHIB7 from Mycobacterium leprae (89 aa), FASTA scores: opt: 441, E(): 6.3e-24, (69.3% identity in 88 aa overlap). Similar to Q9FCJ8|2SC3B6.14 putative DNA-binding protein from Streptomyces coelicolor (122 aa), FASTA scores: opt: 348, E(): 2.2e-17, (57.7% identity in 78 aa overlap); Q9AD55|SCP1.95 putative regulatory protein from Streptomyces coelicolor (102 aa), FASTA scores: opt: 166, E(): 7.1e-05, (39.4% identity in 76 aa overlap); etc. Q6MX01 No regulatory proteins Q6MX01 Q6MX01 GO:0003677 NC_000962.3 Mycobrowser_v5 CDS 3605751 3607034 . - 0 Rv3229c desA3 Thought to be involved in lipid metabolism [catalytic activity: linoleoyl-CoA + ah(2) + O(2) = gamma-linolenoyl-CoA + a + 2 H(2)O] Possible linoleoyl-CoA desaturase (delta(6)-desaturase) Rv3229c, (MTCY20B11.04c), len: 427 aa. DesA3, linoleoyl-CoA desaturase, showing similarity with desaturases and other proteins e.g. Q08871|DES6|SLL0262 linoleoyl-CoA desaturase from Synechocystis sp. strain PCC 6803 (359 aa), FASTA scores: opt: 319, E(): 4e-13, (25.1% identity in 295 aa overlap); Q54795|DESD delta 6 desaturase from Spirulina platensis (368 aa), FASTA scores: opt: 268, E(): 7.7e-10, (25.0% identity in 300 aa overlap); Q9ZTU8|S276 protein with similarity to cytochrome B5 domain from Triticum aestivum (Wheat) (469 aa), FASTA scores: opt: 240, E(): 5.9e-08, (27.05% identity in 266 aa overlap); etc. P9WNZ3 No lipid metabolism Q7D5W1 P9WNZ3 GO:0055114,GO:0016717,GO:0005886,GO:0006633 1.14.19.n4 NC_000962.3 Mycobrowser_v5 CDS 3571602 3572543 . - 0 Rv3199c nudC Involved in nicotinate and nicotinamide metabolism. Generates AMP and NMN from NAD(+) and H(2)O. Acting on acid anhydrides, in phosphorus-containing anhydrides. Also acts on NADP+, 3-acetylpyridine and the thionicotinamide analogues of NAD+ and NADP+ [catalytic activity: NADH + H(2)O = AMP + NMNH]. Probable NADH pyrophosphatase NudC (NAD+ diphosphatase) (NAD+ pyrophosphatase) (NADP pyrophosphatase) Rv3199c, (MTV014.43)c, len: 313 aa. Probable nudC, NADH pyrophosphatase, similar in particular to Q9CXN4|4933433B15RIK from Mus musculus (Mouse) (356 aa), FASTA scores: opt: 493, E(): 7.4e-24, (39.65% identity in 232 aa overlap); Q9ABG1|CC0266 mutt/NUDIX family protein from Caulobacter crescentus (313 aa), FASTA scores: opt: 479, E(): 5.1e-23, (38.3% identity in 222 aa overlap); O86062|NUDC_PSEAE|NUDC|PA1823 NADH pyrophosphatase from Pseudomonas aeruginosa (278 aa), FASTA scores: opt: 371,2 E(): 3e-16, (43.15% identity in 153 aa overlap); Q9RV62|NUDC_DEIRA|NUDC|DR1168 NADH pyrophosphatase from Deinococcus radiodurans (280 aa), FASTA scores: opt: 363, E(): 9.6e-16, (34.45% identity in 270 aa overlap); etc. Caution: equivalent to AAK47636 from Mycobacterium tuberculosis strain CDC1551 (386 aa) but shorter 72 aa. Contains PS00893 mutT domain signature. Belongs to the NUDIX hydrolase family, NUDC subfamily. Cofactor: requires divalent ions: manganese or magnesium. P9WIX5 No intermediary metabolism and respiration O53345 P9WIX5 GO:0000287,GO:0030145,GO:0000210 3.6.1.22 P9WIX5 NC_000962.3 Mycobrowser_v5 CDS 3572602 3573669 . - 0 Rv3200c Rv3200c Thought to be involved in cation transport across the membrane. Possible transmembrane cation transporter Rv3200c, (MTV014.44c), len: 355 aa. Possible transmembrane cation transporter, similar to many transmembrane proteins and putative potassium channels e.g. Q9XA52|SCGD3.27C putative membrane protein from Streptomyces coelicolor (365 aa), FASTA scores: opt: 1022, E(): 2.6e-53, (49.85% identity in 325 aa overlap); Q9RRZ3|DR2336 putative potassium channel from Deinococcus radiodurans (320 aa), FASTA scores: opt: 436, E(): 1e-18, (30.9% identity in 304 aa overlap); O28600|AF1673 putative potassium channel from Archaeoglobus fulgidus (314 aa), FASTA scores: opt: 363, E(): 2.1e-14, (27.2% identity in 309 aa overlap); Q57604|Y13B_METJAMJ0138.1|MJ0138.1 putative potassium channel from Methanococcus jannaschii (333 aa), FASTA scores: opt: 356, E(): 5.7e-14, (26.0% identity in 281 aa overlap); P73132|SLL0993 potassium channel from Synechocystis sp. strain PCC 6803 (365 aa), FASTA scores: opt: 330, E(): 2.1e-12, (27.8% identity in 324 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). O53346 No cell wall and cell processes O53346 O53346 GO:0003824,GO:0016021,GO:0008152,GO:0006813 O53346 NC_000962.3 Mycobrowser_v5 CDS 3582532 3583710 . - 0 Rv3206c moeB1 Possibly involved in molybdopterin metabolism (synthesis) Probable molybdenum cofactor biosynthesis protein MoeB1 (MPT-synthase sulfurylase) (molybdopterin synthase sulphurylase) Rv3206c, (MTCY07D11.20), len: 392 aa. Probable moeB1, molybdopterin cofactor biosynthesis protein, equivalent to Q9CCG8|MOEZ|ML0817 protein probably involved in molybdopterin biosynthesis from Mycobacterium leprae (395 aa), FASTA scores: opt: 2285, E(): 3.3e-130, (86.45% identity in 391 aa overlap.) Very similar to members of the HESA/MOEB/THIF family e.g. Q9FCL0|2SC3B6.02 putative sulfurylase from Streptomyces coelicolor (392 aa), FASTA scores: opt: 1776, E(): 1.4e-99, (65.3% identity in 395 aa overlap); Q9XC37|PDTORFF MOEB-like protein (putative sulfurylase) from Pseudomonas stutzeri (Pseudomonas perfectomarina) (391 aa), FASTA scores: opt: 1526, E(): 1.5e-84, (59.1% identity in 391 aa overlap); O54307|MPT|MOEB MPT-synthase sulfurylase from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (391 aa), FASTA scores: opt: 1309, E(): 1.8e-71, (52.95% identity in 387 aa overlap); P74344|MOEB|SLL1536 molybdopterin biosynthesis MOEB protein from Synechocystis sp. strain PCC 6803 (392 aa), FASTA scores: opt: 1308, E(): 2e-71, (50.65% identity in 397 aa overlap); etc. Also highly similar to O05792|MOEB2|Rv3116|MTCY164.26 putative molybdenum cofactor biosynthesis protein from Mycobacterium tuberculosis (389 aa), FASTA scores: opt: 1440, E(): 2.3e-79, (57.25% identity in 386 aa overlap). Has hydrophobic segment from ~45-71. Belongs to the HesA/MoeB/ThiF FAMILY. Note that previously known as moeZ. Thought to be differentially expressed within host cells (see citation below). P9WMN7 No intermediary metabolism and respiration Q7D5X9 P9WMN7 GO:0003824,GO:0008152 P9WMN7 NC_000962.3 Mycobrowser_v5 CDS 3583801 3584658 . - 0 Rv3207c Rv3207c Function unknown Conserved protein Rv3207c, (MTCY07D11.19), len: 285 aa. Conserved protein, highly similar but shorter (57 aa) to Q9CCG9|ML0816 hypothetical protein from Mycobacterium leprae (341 aa), FASTA scores: opt: 1676, E(): 9.7e-96, (81.0% identity in 284 aa overlap). Also similar to C-terminus of Q9FBI6|SCP8.36 hypothetical protein from Streptomyces coelicolor (559 aa), FASTA scores: opt: 426, E(): 8.4e-19, (37.35% identity in 281 aa overlap); and similar to other hypothetical proteins (generally membrane proteins) e.g. Q9K456|SC2H12.28C putative membrane protein from Streptomyces coelicolor (314 aa), FASTA scores: opt: 341, E(): 8.8e-14, (29.75% identity in 296 aa overlap). Contains neutral zinc metallopeptidases, zinc-binding region signature (PS00142). O05859 No conserved hypotheticals O05859 O05859 GO:0006508,GO:0008270,GO:0008237 O05859 NC_000962.3 Mycobrowser_v5 CDS 3585004 3585690 . + 0 Rv3208 Rv3208 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably TetR-family) Rv3208, (MTCY07D11.18c), len: 228 aa. Probable transcriptional regulator, TetR family, equivalent to Q9CCH0|ML0815 putative TetR-family transcriptional regulator from Mycobacterium leprae (228 aa), FASTA scores: opt: 1248, E(): 1.4e-74, (82.4% identity in 227 aa overlap). Also highly similar to Q9FBI8|SCP8.33c putative TetR-family transcriptional regulator from Streptomyces coelicolor (213 aa), FASTA scores: opt: 629, E(): 4e-34, (45.8% identity in 203 aa overlap); Q9KIL9|F58R F58R (fragment) from Streptomyces coelicolor A3(2) (149 aa), FASTA scores: opt: 497, E(): 1.3e-25, (50.35% identity in 147 aa overlap); Q9K3T5|SCE66.08 putative TetR-family transcriptional regulator from Streptomyces coelicolor (225 aa), FASTA scores: opt: 344, E(): 1.8e-15, (31.15% identity in 212 aa overlap); Q9RYK4|DRA0308 transcriptional regulator, TetR family from Deinococcus radiodurans (239 aa), FASTA scores: opt: 290, E(): 6.5e-12, (30.5% identity in 223 aa overlap); etc. And also similar to Mycobacterium tuberculosis proteins P96381|Rv1019|MTCY10G2.30c hypothetical 21.7 KDA protein (197 aa), FASTA scores: opt: 356, E(): 2.7e-16, (34.4% identity in 189 aa overlap); MTV034_4; MTY07A7A_3; MTV032_1; MTCY07A7_12; etc. Contains probable helix-turn-helix motif at aa 60-81 (Score 1517, +4.35 SD). Similar to the TetR/AcrR family of transcriptional regulators. O05858 No regulatory proteins O05858 O05858 GO:0006350,GO:0003700,GO:0006355 O05858 NC_000962.3 Mycobrowser_v5 CDS 3736984 3738438 . - 0 Rv3344c PE_PGRS49 Function unknown PE-PGRS family protein PE_PGRS49 Rv3344c, (MTV016.44c), len: 484 aa. PE_PGRS49, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-, ala-rich proteins (see Brennan and Delogu, 2002). Appears to be a gene fragment, should be in-frame with following ORF, MTV016.45c, frameshift required around 49595 but could not be found on checking BAC and cosmid clones. Similar to many from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. O53557|Rv3512|MTV023.19 (1079 aa), FASTA scores: opt: 1595, E(): 1.8e-54, (52.0% identity in 544 aa overlap). L0TFC2 No PE/PPE L0TFC2 NC_000962.3 Mycobrowser_v5 CDS 3587798 3589381 . + 0 Rv3211 rhlE Has a helix-destabilizing activity Probable ATP-dependent RNA helicase RhlE Rv3211, (MTCY07D11.15c), len: 527 aa. Probable rhlE, ATP-dependent RNA helicase, equivalent (but shorter 22 aa) to Q9CCH3|RHLE|ML0811 putative ATP-dependent RNA helicase from Mycobacterium leprae (544 aa), FASTA scores: opt: 2497, E(): 8.7e-131, (74.75% identity in 531 aa overlap). Also highly similar to other RNA helicases e.g. Q9FBJ2|SCP8.29c from Streptomyces coelicolor (879 aa), FASTA scores: opt: 1458, E(): 3.6e-73, (52.5% identity in 522 aa overlap); Q9DF36 from Xenopus laevis (African clawed frog) (800 aa), FASTA scores: opt: 792, E(): 2.3e-36, (37.15% identity in 385 aa overlap); Q99Z38|dead|SPY1415 from Streptococcus pyogenes (759 aa), FASTA scores: opt: 779, E(): 1.1e-35, (37.1% identity in 380 aa overlap); P33906|dead|CSDA from Klebsiella pneumoniae (642 aa), FASTA scores: opt: 768, E(): 4e-35, (43.4% identity in 387 aa overlap); etc. Contains ATP/GTP-binding site motif A (PS00017) and dead-box subfamily ATP-dependent helicases signature (PS00039). Similar to dead/DEAH box helicase family and similar to helicase C-terminal domain. O05855 No information pathways O05855 O05855 GO:0008026,GO:0003676,GO:0005524 O05855 NC_000962.3 Mycobrowser_v5 CDS 3589394 3590617 . + 0 Rv3212 Rv3212 Function unknown Conserved alanine valine rich protein Rv3212, (MTCY07D11.14c), len: 407 aa. Conserved ala-, val-rich protein, equivalent to Q9CCH4|ML0810 putative membrane protein from Mycobacterium leprae (407 aa), FASTA scores: opt: 2158, E(): 5.3e-119, (79.85% identity in 407 aa overlap). Weak similarity to several eukaryotic transcription factors e.g. P08393|ICP0_HSV11|ICP0|IE110 trans-acting transcriptional protein from Herpes simplex virus (type 1 / strain 17) (775 aa), FASTA scores: opt: 115, E(): 2, (26.9% identity in 334 aa overlap). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). Predicted to be an outer membrane protein (See Song et al., 2008). O05854 No conserved hypotheticals O05854 NC_000962.3 Mycobrowser_v5 CDS 3590692 3591492 . - 0 Rv3213c Rv3213c Function unknown, but possibly involved in regulation of partitioning. Possible SOJ/para-related protein Rv3213c, (MTCY07D11.13), len: 266 aa. Possible soj/parA-related protein, very similar in particular to Soj/ParA proteins (and relatives) from Bacillus subtilis that inhibit the initiation of sporulation by preventing phosphorylation of Spo0A (see Quisel & Grossman 2000) e.g. Q9S228|SCI51.12c from Streptomyces coelicolor (340 aa), FASTA scores: opt: 746, E(): 1.6e-40, (48.2% identity in 249 aa overlap); Q9HT11|SOJ|PA5563 from Pseudomonas aeruginosa (262 aa), FASTA scores: opt: 649, E(): 2.1e-34, (42.2% identity in 256 aa overlap); Q9PB62|XF2282 from Xylella fastidiosa (264 aa), FASTA scores: opt: 624, E(): 8.3e-33, (42.25% identity in 251 aa overlap); Q9K5N0|SOJ_BACHD|SOJ|BH4058 from Bacillus halodurans (253 aa), FASTA scores: opt: 621, E(): 1.2e-32, (41.55% identity in 248 aa overlap); P37522|SOJ_BACSU (253 aa), FASTA scores: opt: 620, E(): 1.4e-32, (41.65% identity in 245; etc. Also similar to various mycobacterial proteins: U00021_10 from Mycobacterium leprae, MTCI125_29 from Mycobacterium tuberculosis, MLCB1351_6 from Mycobacterium leprae, MTV028_9c|Rv3918c|para probable chromosome partitioning protein from Mycobacterium tuberculosis, MSGDNAB_18 from Mycobacterium leprae. Seems to belong to the para family. O05853 No cell wall and cell processes O05853 O05853 O05853 NC_000962.3 Mycobrowser_v5 CDS 3591646 3592257 . + 0 Rv3214 gpm2 Involved in glycolysis [catalytic activity: 1,3-diphosphoglycerate + 3-phosphoglycerate = 2,3-diphosphoglycerate + 3-phosphoglycerate]. Possible phosphoglycerate mutase Gpm2 (phosphoglyceromutase) (PGAM) (BPG-dependent PGAM) Rv3214, (MTCY07D11.12c), len: 203 aa. Possible gpm2, phosphoglycerate mutase, similar to many mutases especially phosphoglycerate mutases e.g. Q9F3H5|2SCC13.14c putative mutase from Streptomyces coelicolor (198 aa), FASTA scores: opt: 487, E(): 4.4e-25, (42.25% identity in 194 aa overlap); BAB49378|MLL2186 probable phosphoglycerate mutase from Rhizobium loti (Mesorhizobium loti) (193 aa), FASTA scores: opt: 423, E(): 7e-21, (41.2% identity in 182 aa overlap); Q9RKV8|SC9G1.08c putative phosphatase from Streptomyces coelicolor (199 aa), FASTA scores: opt: 419, E(): 1.3e-20, (41.1% identity in 185 aa overlap); Q9RDL0|SCC123.14c putative phosphoglycerate mutase from Streptomyces coelicolor (223 aa), FASTA scores: opt: 240, E(): 8.8e-09, (36.9% identity in 168 aa overlap); Q9X194|TM1374 phosphoglycerate mutase from Thermotoga maritima (201 aa), FASTA scores: opt: 218, E(): 2.3e-07, (33.15% identity in 202 aa overlap); etc. But N-terminus also similar to Q9CCH5|ENTC|ML0808 putative isochorismate synthase from Mycobacterium leprae (577 aa), FASTA scores: opt: 346, E(): 2.1e-15, (55.05% identity in 109 aa overlap). N-terminus shows also some similarity with other M. tuberculosis proteins e.g. MTCY427.09c; MTCY20G9.15; MTCY428.28. Equivalent to AAK47652 from Mycobacterium tuberculosis strain CDC1551 (228 aa) but shorter 25 aa. Note that previously known as entD. Q6MWZ7 No intermediary metabolism and respiration 2A6P Q6MWZ7 Q6MWZ7 GO:0004619 5.4.2.1 Q6MWZ7 NC_000962.3 Mycobrowser_v5 CDS 3595713 3595967 . + 0 Rv3219 whiB1 Involved in transcriptional mechanism. Transcriptional regulatory protein WhiB-like WhiB1. Contains [4FE-4S]2+ cluster. Rv3219, (MTCY07D11.07c), len: 84 aa. WhiB1 (alternate gene name: whmE), WhiB-like regulatory protein (see Hutter and Dick, 1999), similar to WhiB paralogue of Streptomyces coelicolor. Equivalent to Q9CCH7|WHIB1|ML0804 putative transcriptional regulator from Mycobacterium leprae (84 aa), FASTA scores: opt: 580, E(): 3.5e-35, (95.25% identity in 84 aa overlap). Highly similar to several e.g. Q9X952|WBLE developmental regulatory protein WhiB-paralog from Streptomyces coelicolor (85 aa), FASTA scores: opt: 477, E(): 9.2e-28, (75.3% identity in 81 aa overlap); Q9AD55|SCP1.95 putative regulatory protein from Streptomyces coelicolor (102 aa), FASTA scores: opt: 383, E(): 6.1e-21, (60.75% identity in 79 aa overlap); Q9K4K8|SC5F8.16c from Streptomyces coelicolor (83 aa), FASTA scores: opt: 346, E(): 2.5e-18, (54.75% identity in 84 aa overlap); etc. P9WF43 No regulatory proteins O05847 P9WF43 P9WF43 NC_000962.3 Mycobrowser_v5 CDS 3611959 3613116 . - 0 Rv3236c Rv3236c Probably involved in transport of undetermined substrate (possibly cations Na/H) across the membrane. Thought to be responsible for the translocation of the substrate across the membrane. Probable conserved integral membrane transport protein Rv3236c, (MTCY20B11.11c), len: 385 aa. Probable conserved integral membrane transport protein, possibly cation (Na/H) transporter, equivalent to Q9CCI5|ML0782 putative transmembrane transport protein from Mycobacterium leprae (385 aa), FASTA scores: opt: 1975, E(): 2.4e-108, (81.55% identity in 385 aa overlap). Highly similar to others e.g. O69958|SC4H2.03c putative transmembrane transport protein from Streptomyces coelicolor (411 aa), FASTA scores: opt: 1226, E(): 1.6e-64, (53.5% identity in 372 aa overlap); Q9XAKO|SC66T3.13c putative transmembrane transport protein from Streptomyces coelicolor (403 aa), FASTA scores: opt: 1198, E(): 6.8e-63, (53.25% identity in 370 aa overlap); Q9RV80|DR1149 putative Na+/H+ antiporter from Deinococcus radiodurans (383 aa), FASTA scores: opt: 1069, E(): 2.3e-55, (47.35% identity in 376 aa overlap); Q9L191|SC10G8.11 putative transmembrane transport protein from Streptomyces coelicolor (446 aa), FASTA scores: opt: 695, E(): 1.9e-33, (38.05% identity in 384 aa overlap); Q9RRW8|DR2367 putative glutathione-regulated potassium-efflux system protein KEFB from Deinococcus radiodurans (575 aa), FASTA scores: opt: 414, E(): 6.2e-17, (30.25% identity in 380 aa overlap); etc. Seems to belong to the CPA2 family. Note that previously known as kefB. L7N665 No cell wall and cell processes Q7D5V5 L7N665 GO:0016021,GO:0015299,GO:0055085,GO:0006812 L7N665 NC_000962.3 Mycobrowser_v5 CDS 3599851 3600699 . + 0 Rv3224 Rv3224 Function unknown; probably involved in cellular metabolism. Possible iron-regulated short-chain dehydrogenase/reductase Rv3224, (MTCY07D11.02c), len: 282 aa. Probable iron-regulated oxidoreductase, possible short-chain dehydrogenase/reductase, highly similar to BAB49551|MLL2413 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (288 aa), FASTA scores: opt: 1053, E(): 6.4e-59, (57.95% identity in 276 aa overlap); Q9AB34|CC0400 short chain dehydrogenase family protein from Caulobacter crescentus (285 aa), FASTA scores: opt: 1051, E(): 8.5e-59, (55.9% identity in 281 aa overlap); and Q9VB10|CG5590 hypothetical protein (similar to the short-chain dehydrogenases/reductases (SDR) family) from Drosophila melanogaster (Fruit fly) (412 aa), FASTA scores: opt: 966, E(): 2.5e-53, (52.15% identity in 278 aa overlap). Similar to various proteins (principaly oxidoreductases) e.g. Q18639|C45B11.3 hypothetical protein (similar to the SDR family) from Caenorhabditis elegans (293 aa), FASTA scores: opt: 921, E(): 1.2e-50, (51.3% identity in 271 aa overlap); Q9HZV5|PA2892 probable short-chain dehydrogenase from Pseudomonas aeruginosa (274 aa), FASTA scores: opt: 847, E(): 5.1e-46, (49.25% identity in 274 aa overlap); Q9I6V0|PA0182 probable short-chain dehydrogenase (similar to the SDR family) from Pseudomonas aeruginosa (250 aa), FASTA scores: opt: 333, E(): 8.3e-14, (29.8% identity in 245 aa overlap); Q9HY98|PA3511 probable short-chain dehydrogenase from Pseudomonas aeruginosa (253 aa), FASTA scores: opt: 330, E(): 1.3e-13, (31.2% identity in 250 aa overlap); etc. Related proteins in Mycobacterium tuberculosis include MTCY02B10.14, MTCY369.14, and MTCY09F9.36. Has ATP/GTP-binding site motif A, (PS00017) near C-terminus. May be belong to the short-chain dehydrogenases/reductases (SDR) family. O05842 No intermediary metabolism and respiration O05842 O05842 GO:0055114,GO:0016491 1.-.-.- NC_000962.3 Mycobrowser_v5 CDS 3607112 3608254 . - 0 Rv3230c Rv3230c Function unknown; probably involved in cellular metabolism. Hypothetical oxidoreductase Rv3230c, (MTCY20B11.05c), len: 380 aa. Putative oxidoreductase, with some similarity to various proteins, especially reductases e.g. Q9HUS4|PA4889 probable oxidoreductase from Pseudomonas aeruginosa (366 aa), FASTA scores: opt: 516, E(): 1.8e-24, (33.8% identity in 367 aa overlap); P95533|TDNB electron transfer protein from Pseudomonas putida (337 aa), FASTA scores: opt: 380, E(): 4e-16, (30.7% identity in 277 aa overlap); BAB34381|ECS0958 NADH oxidoreductase for the HCP from Escherichia coli strain O157:H7 (322 aa), FASTA scores: opt: 369, E(): 1.8e-15, (28.65% identity in 328 aa overlap); Q44253|ATDA5 aniline dioxygenase reductase component from Acinetobacter sp. (336 aa), FASTA scores: opt: 305, E(): 1.6e-11, (27.4% identity in 303 aa overlap); etc. P9WNE9 No intermediary metabolism and respiration O05875 P9WNE9 GO:0005737,GO:0009055,GO:0022900,GO:0046872,GO:0016491,GO:0006810,GO:0051537 P9WNE9 NC_000962.3 Mycobrowser_v5 CDS 3609781 3610371 . - 0 Rv3233c Rv3233c May be involved in synthesis of triacylglycerol Possible triacylglycerol synthase (diacylglycerol acyltransferase) Rv3233c, (MTCY20B11.08c), len: 196 aa. Possible triacylglycerol synthase (See Daniel et al., 2004), similar to C-terminus of Q9RIU8|SCM11.13c hypothetical 47.1 KDA protein from Streptomyces coelicolor (446 aa), FASTA scores: opt: 308, E(): 1.2e-12, (32.0% identity in 200 aa overlap); and several hypothetical M. tuberculosis proteins e.g. O06343|YY80_MYCTU|Rv3480c|MTCY13E12.33c (497 aa), FASTA scores: opt: 248, E(): 9.8e-09, (27.5% identity in 200 aa overlap); MTCY28_26; MTCY493_29; MTCY31_25; MTCY31_25. O05878 No lipid metabolism O05878 O05878 NC_000962.3 Mycobrowser_v5 CDS 3614457 3617603 . - 0 Rv3239c Rv3239c Unknown, but seems involved in efflux system (probably sugar or drug transport). Probable conserved transmembrane transport protein Rv3239c, (MTCY20B11.14c), len: 1048 aa. Probable conserved transmembrane protein, organised in two domains. Domain comprising first ~500 aa residues is similar to various antibiotic resistance and efflux proteins and contains sugar transport proteins signature 1 (PS00216); e.g. Q9RL22|SC5G9.04c putative transmembrane efflux protein from Streptomyces coelicolor (489 aa), FASTA scores: opt: 905, E(): 3.1e-41, (36.95% identity in 482 aa overlap); and O68912|FRNF putative antibiotic antiporter from Streptomyces roseofulvus (517 aa), FASTA scores: opt: 866, E(): 4.1e-39, (37.1% identity in 512 aa overlap). Second part, corresponding to last 550 aa residues, is very similar to Q50733|Rv2565|MTCY9C4.03c hypothetical 62.1 kDa protein from Mycobacterium tuberculosis (583 aa), FASTA scores: E(): 2.1e-28, (36.5% identity in 572 aa overlap). Also equivalent to Rv3728|MTV025.076 putative two-domain membrane protein (similar to sugar transporter family) from Mycobacterium tuberculosis (1065 aa), FASTA scores: opt: 4328, E(): 0, (64.15% identity in 1046 aa overlap); and similar to other Mycobacterium tuberculosis proteins: MTCY3G12.01, E(): 6.3e-32; MTCY98.02c, E(): 6.3e-32; MTCY9C4.03c, E(): 1.5e-26; MTCY369.27c, E(): 2.5e-26. Equivalent to AAK47679 Drug transporter from Mycobacterium tuberculosis strain CDC1551 (1065 aa) but shorter 20 aa. Contains cyclic nucleotide-binding domain signature 2 (PS00889). Probably member of major facilitator superfamily (MFS). O05884 No cell wall and cell processes O05884 O05884 GO:0016021,GO:0004622,GO:0046470,GO:0046677,GO:0015904,GO:0015520,GO:0055085,GO:0005789 O05884 NC_000962.3 Mycobrowser_v5 CDS 3624910 3626613 . - 0 Rv3245c mtrB Sensor part of a two component regulatory system. Two component sensory transduction histidine kinase MtrB Rv3245c, (MTCY20B11.20c), len: 567 aa. MtrB, sensor-like histidine kinase (see citations below), equivalent to Q9CCJ1|MTRB or ML0774 putative two-component system sensor kinase from Mycobacterium leprae (562 aa), FASTA scores: opt: 3208, E(): 7.4e-173, (88.7% identity in 566 aa overlap). Also similar to others e.g. Q9KYW9|SCE33.14c putative two-component system histidine kinase from Streptomyces coelicolor (688 aa), FASTA scores: opt: 1355, E(): 1.1e-68, (48.95% identity in 515 aa overlap); etc. Relatives in Mycobacterium tuberculosis are: MTCY369.03, E(): 1.5e-22; MTCY20G9.16, E(): 1.9e-17. Similar to other prokaryotic sensory transduction histidine kinases. P9WGK9 No regulatory proteins Q50496 P9WGK9 GO:0016021,GO:0018106,GO:0005886,GO:0007165,GO:0000155,GO:0000160,GO:0005524 2.7.13.3 P9WGK9 NC_000962.3 Mycobrowser_v5 CDS 3626663 3627349 . - 0 Rv3246c mtrA Transcriptional activator part of a two component regulatory system. Two component sensory transduction transcriptional regulatory protein MtrA Rv3246c, (MTCY20B11.21c), len: 228 aa. MtrA, transcriptional activator, response regulator (see citations below), equivalent to Q9CCJ2|MTRA|ML0773 putative two-component response regulator from Mycobacterium leprae (228 aa), FASTA scores: opt: 1458, E(): 1.4e-85, (98.7% identity in 228 aa overlap). Also highly similar to others e.g. Q9F9J5|SCRA putative response regulator from Streptomyces coelicolor (228 aa), FASTA scores: opt: 1141, E(): 1.9e-65, (74.9% identity in 227 aa overlap); Q9KYW8|SCE33.15c putative two-component system response regulator from Streptomyces coelicolor (229 aa), FASTA scores: opt: 1141, E(): 1.9e-65, (74.9% identity in 227 aa overlap); Q9F868|REGX3 response regulator REGX3 from Mycobacterium smegmatis (228 aa), FASTA scores: opt: 730, E(): 2.3e-39, (50.90% identity in 222 aa overlap); etc. Relatives in Mycobacterium tuberculosis are: U01971|MTU01971_1; Q11156|RGX3_MYCTU; MTCY20G9.17, E(): 0; MTCY31.31c, E(): 3.4e-29; MTCY369.02, E(): 5.7e-28. Similar to bacterial regulatory proteins involved in signal transduction. The N-terminal region is similar to that of other regulatory components of sensory transduction systems. Experiments showed mtrA is differentially expressed in virulent and avirulent strains during growth in macrophages. P9WGM7 No regulatory proteins 2GWR,3NHZ P0A5Z4 P9WGM7 GO:0006355,GO:0006350,GO:0000156,GO:0000160,GO:0003677 P9WGM7 NC_000962.3 Mycobrowser_v5 CDS 3643177 3644838 . + 0 Rv3263 Rv3263 Causes DNA methylation. Probable DNA methylase (modification methylase) (methyltransferase) Rv3263, (MTCY71.03), len: 553 aa. Probable DNA methylase, equivalent to Q9CCK4|ML0756 probable DNA methylase from Mycobacterium leprae (555 aa), FASTA scores: opt: 2980, E(): 2.1e-184, (81.9% identity in 541 aa overlap). Also similar to others e.g. P25240|MT57_ECOLI|ECO57IM modification methylase from Escherichia coli (544 aa), FASTA scores: opt: 595, E(): 1e-30, (30.35% identity in 507 aa overlap); P25201|MTA1_ACICA|ACCIM modification methylase ACCI from Acinetobacter calcoaceticus (540 aa), FASTA scores: opt: 366, E(): 5.7e-16, (23.35% identity in 467 aa overlap); Q56752|M-ACCI ACCI methylase from Bergeyella zoohelcum (541 aa), FASTA scores: opt: 365, E(): 6.6e-16, (22.95% identity in 466 aa overlap); etc. Contains PS00092 N-6 Adenine-specific DNA methylases signature. Alternative start site at aa 25. P96868 No information pathways P96868 P96868 GO:0006306,GO:0008170,GO:0009007,GO:0003677 2.1.1.72 P96868 NC_000962.3 Mycobrowser_v5 CDS 3628160 3629647 . - 0 Rv3248c sahH Thioester hydrolase which acting on ether bounds. Could be involved in methionine and selenoamino acid metabolisms. Also involved in activated methyl. Cycle adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine [catalytic activity: S-adenosyl-L-homocysteine + H(2)O = adenosine + L-homocysteine]. Probable adenosylhomocysteinase SahH (S-adenosyl-L-homocysteine hydrolase) (adohcyase) Rv3248c, (MTCY20B11.23c), len: 495 aa. Probable sahH, adenosylhomocysteinase, equivalent to Q9CCJ4|SAHH|ML0771 putative S-adenosyl-L-homocysteine hydrolase from Mycobacterium leprae (492 aa), FASTA scores: opt: 3019, E(): 1.3e-177, (91.4% identity in 489 aa overlap). Also highly similar to other adenosylhomocysteinases e.g. Q9KZM1|SAHH from Streptomyces coelicolor (485 aa), FASTA scores: opt: 2258, E(): 5.7e-131, (70.0% identity in 483 aa overlap); P51540|SAHH_TRIVA from Trichomonas vaginalis (486 aa), FASTA scores: opt: 2005, E(): 1.8e-115, (62.05% identity in 477 aa overlap); P35007|SAHH_CATRO from Catharanthus roseus (Rosy periwinkle) (Madagascar periwinkle) (485 aa), FASTA scores: opt: 1941, E(): 1.5e-111, (60.15% identity in 492 aa overlap); etc. Has S-adenosyl-L-homocysteine hydrolase signature (PS00739). Belongs to the adenosylhomocysteinase family. P9WGV3 No intermediary metabolism and respiration 2ZIZ,2ZJ0,2ZJ1,3CE6,3DHY P60176 P9WGV3 GO:0005737,GO:0006730,GO:0004013 3.3.1.1 P9WGV3 NC_000962.3 Mycobrowser_v5 CDS 3630738 3631988 . - 0 Rv3252c alkB Thought to be involved in fatty acid metabolism. Generates octanol and oxidized rubredoxin from octane and reduced rubredoxin. Also hydroxylates fatty acids in the omega-position [catalytic activity: octane + reduced rubredoxin + (O)2 = 1-octanol + oxidized rubredoxin + H(2)O]. Probable transmembrane alkane 1-monooxygenase AlkB (alkane 1-hydroxylase) (lauric acid omega-hydroxylase) (omega-hydroxylase) (fatty acid omega-hydroxylase) (alkane hydroxylase-rubredoxin) Rv3252c, (MTCY20B11.27c), len: 416 aa. Probable alkB, transmembrane alkane-1-monooxygenase, highly similar to many (see Marin et al., 2001) e.g. Q9AE68|ALKB2 from Rhodococcus erythropolis (408 aa), FASTA scores: opt: 2018, E(): 9.6e-122, (68.6% identity in 415 aa overlap); Q9AFD5|ALKB from Nocardioides sp. CF8 (483 aa), FASTA scores: opt: 1485, E(): 1.4e-87, (56.55% identity in 405 aa overlap); Q9XAU0|ALKB1 from Rhodococcus erythropolis (391 aa), FASTA scores: opt: 1400, E(): 3.3e-82, (62.6% identity in 396 aa overlap); Q9XBM1|ALKB from Prauserella rugosa (490 aa), FASTA scores: opt: 1266, E(): 1.5e-73, (57.55% identity in 410 aa overlap); CAC40954|ALKB4 from Rhodococcus erythropolis (386 aa), FASTA scores: opt: 1190, E(): 9.1e-69, (54.3% identity in 383 aa overlap); etc. O05895 No lipid metabolism O05895 O05895 GO:0016021,GO:0006629,GO:0055114,GO:0018685 1.14.15.3 NC_000962.3 Mycobrowser_v5 CDS 3635041 3636267 . - 0 Rv3255c manA This enzyme converts D-mannose 6-phosphate to D-fructose 6-phosphate [catalytic activity: D-mannose 6-phosphate = D-fructose 6-phosphate]. Probable mannose-6-phosphate isomerase ManA (phosphomannose isomerase) (phosphomannoisomerase) (PMI) (phosphohexoisomerase) (phosphohexomutase) Rv3255c, (MTCY20B11.30c), len: 408 aa. Probable manA, mannose-6-phosphate isomerase, equivalent to Q9CCJ5|MANA|ML0765 putative mannose-6-phosphate isomerase from Mycobacterium leprae (410 aa), FASTA scores: opt: 2271, E(): 1.6e-133, (84.45% identity in 411 aa overlap). Also similar to many others e.g. Q9KZL9|MANA from Streptomyces coelicolor (383 aa), FASTA scores: opt: 946, E(): 2.4e-51, (44.4% identity in 403 aa overlap); Q9KV87|VC0269 from Vibrio cholerae (399 aa), FASTA scores: opt: 726, E(): 1.1e-37, (34.15% identity in 404 aa overlap); Q9CMJ5|PMI|PM0829 from Pasteurella multocida (400 aa), FASTA scores: opt: 640, E(): 2.4e-32, (32.5% identity in 391 aa overlap); etc. Similar to family 1 of mannose-6-phosphate isomerases. O05898 No intermediary metabolism and respiration O05898 O05898 GO:0004476,GO:0008270,GO:0005975 5.3.1.8 O05898 NC_000962.3 Mycobrowser_v5 CDS 3640543 3641538 . + 0 Rv3261 fbiA Required for coenzyme F420 production: involved in the conversion of FO into F420. Probable F420 biosynthesis protein FbiA Rv3261, (MTCY71.01), len: 331 aa. Probable fbiA, F420 biosynthesis protein, equivalent to FBIA F420 biosynthesis protein fbiA from Mycobacterium bovis BCG (see citations below). Also equivalent, but shorter 46 aa, to Q9CCK1|ML0759 hypothetical protein from Mycobacterium leprae (379 aa), FASTA scores: opt: 1855, E(): 3.9e-110, (79.3% identity in 333 aa overlap). Also similar to others e.g. Q9KZK9|SCE34.17 hypothetical 33.6 KDA protein from Streptomyces coelicolor (319 aa), FASTA scores: opt: 1151, E(): 1.2e-65, (55.1% identity in 332 aa overlap); O29345|AF0917 conserved hypothetical protein from Archaeoglobus fulgidus (296 aa), FASTA scores: opt: 469, E(): 1.7e-22, (31.15% identity in 302 aa overlap); Q58653|MJ1256 hypothetical protein from Methanococcus jannaschii (311 aa), FASTA scores: opt: 436, E(): 2.2e-20, (27.35% identity in 274 aa overlap); etc. P9WP81 No intermediary metabolism and respiration P96866 P9WP81 GO:0000287,GO:0016773,GO:0016780,GO:0009108 2.7.8.- P9WP81 NC_000962.3 Mycobrowser_v5 CDS 3644898 3645977 . - 0 Rv3264c manB Involved in GDP-mannose biosynthesis and biosynthesis of nucleotide-activated glycero-manno-heptose (D-alpha-D pathway): generates GDP-mannose and phosphate from GTP and alpha-D-mannose 1-phosphate. MANB product is needed for all mannosyl glycolipids and polysaccharides which, like rhamnosyl residues, are an important part of the mycobacterium envelope [catalytic activity: alpha-D-mannose 1-phosphate + GTP = GDP-mannose + phosphate]. D-alpha-D-mannose-1-phosphate guanylyltransferase ManB (D-alpha-D-heptose-1-phosphate guanylyltransferase) Rv3264c, (MTCY71.04c), len: 359 aa. ManB (alternate gene name: hddC), D-alpha-D-mannose-1-phosphate guanylyltransferase (see citations below), equivalent to Q9CCK6|RMLA2|ML0753 putative sugar-phosphate nucleotidyl transferase from Mycobacterium leprae (358 aa), FASTA scores: opt: 2075, E(): 2.7e-115, (86.9% identity in 359 aa overlap). Also similar to others e.g. Q9KZK6|SCE34.20c putative nucleotide phosphorylase from Streptomyces coelicolor (360 aa), FASTA scores: opt: 1314, E(): 2.2e-70, (57.0% identity in 358 aa overlap); Q9KZP4|SC1A8A.08 putative mannose-1-phosphate guanyltransferase from Streptomyces coelicolor (831 aa), FASTA scores: opt: 699, E(): 8.6e-34, (34.45% identity in 354 aa overlap) (only similarity in N-terminus for this one); P74589|SLL1496 mannose-1-phosphate guanyltransferase from Synechocystis sp. strain PCC 6803 (843 aa), FASTA scores: opt: 692, E(): 2.3e-33, (35.1% identity in 342 aa overlap) (only similarity in N-terminus for this one too); BAB59222|TVG0079558 mannose-1-phosphate guanyltransferase from Thermoplasma volcanium (359 aa), FASTA scores: opt: 664, E(): 5.2e-32, (34.6% identity in 338 aa overlap); Q9ZTW5|GMP GDP-mannose pyrophosphorylase from Solanum tuberosum (Potato) (361 aa), FASTA scores: opt: 636, E(): 2.3e-30, (34.65% identity in 361 aa overlap); etc. Belongs to family 2 of mannose-6-phosphate isomerases. Note that previously known as rmlA2. L7N6A5 No cell wall and cell processes Q7D5T3 L7N6A5 GO:0004475,GO:0009058 2.7.7.13 EMB L7N6A5 NC_000962.3 Mycobrowser_v5 CDS 3650234 3650515 . + 0 Rv3269 Rv3269 Function unknown. May be involved in a chaperoning process. Conserved protein Rv3269, (MTCY71.09), len: 93 aa. Conserved protein, similar to many Mycobacterium proteins and chaperonins/heat shock proteins e.g. Q9CCL0|ML0748 hypothetical protein from Mycobacterium leprae (92 aa), FASTA scores: opt: 427, E(): 6.8e-21, (73.65% identity in 91 aa overlap); Q10865|Rv1993c|MT2049|MTCY39.26c hypothetical protein from Mycobacterium tuberculosis (90 aa), FASTA scores: opt: 313, E(): 1.2e-13, (60.7% identity in 84 aa overlap); P71542|Y968_MYCTU|Rv0968|MTCY10D7.06c (98 aa), FASTA scores: opt: 294, E(): 2.2e-12, (55.1% identity in 98 aa overlap); Q50827|MOPA|GROEL|CH60_MYCVA chaperonin (protein CPN60) from Mycobacterium vaccae (120 aa), FASTA scores: opt: 107, E(): 2.1, (39.5% identity in 81 aa overlap); Q9AEB3|HSP65 heat shock protein (fragment) from Mycobacterium gadium (122 aa), FASTA scores: opt: 102, E(): 4.4, (38.25% identity in 81 aa overlap); Q49374|CH60_MYCGN|MOPA|GROEL chaperonin (protein CPN60) from Mycobacterium genavense (120 aa), FASTA scores: opt: 99, E(): 6.8, (40.25% identity in 82 aa overlap); etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). P96874 No virulence, detoxification, adaptation P96874 P96874 NC_000962.3 Mycobrowser_v5 CDS 3653448 3654632 . + 0 Rv3272 Rv3272 Function unknown Conserved hypothetical protein Rv3272, (MTCY71.12), len: 394 aa. Conserved hypothetical protein, similar to various proteins e.g. Q9I672|PA0446 hypothetical protein from Pseudomonas aeruginosa (407 aa), FASTA scores: opt: 643, E(): 6.8e-32, (33.15% identity in 389 aa overlap); Q9RJU8|SCF41.21 putative racemase from Streptomyces coelicolor (403 aa), FASTA scores: opt: 541, E(): 1.1e-25, (31.95% identity in 385 aa overlap); O87838|SC8A6.04c putative transferase from Streptomyces coelicolor (410 aa), FASTA scores: opt: 539, E(): 1.5e-25, (29.95% identity in 395 aa overlap); Q9I563|PA0882 from Pseudomonas aeruginosa (400 aa), FASTA scores: opt: 530, E(): 5.2e-25, (28.8% identity in 396 aa overlap); BAB60328|TVG1215416 L-carnitine dehydratase from Thermoplasma volcanium (399 aa), FASTA scores: opt: 529, E(): 6e-25, (32.9% identity in 383 aa overlap); etc. C-terminus is similar to Q49678|U00012_27|B1308_C3_195 from Mycobacterium leprae (130 aa) (60.0% identity in 115 aa overlap). Also partially similar to MTCY359_7 from M. tuberculosis (778 aa) (29.9% identity in 388 aa overlap). P96877 No conserved hypotheticals P96877 P96877 GO:0008152,GO:0003824 P96877 NC_000962.3 Mycobrowser_v5 CDS 3654637 3656931 . + 0 Rv3273 Rv3273 Generates CO(2) and H(2)O from H(2)co(3), and possibly involved in transport of sulfate across the membrane. Probable transmembrane carbonic anhydrase (carbonate dehydratase) (carbonic dehydratase) Rv3273, (MTCY71.13), len: 764 aa. Probable transmembrane protein (N-terminal part is hydrophobic) with probable carbonic anhydrase activity (in C-terminal part). Possibly involved in transport of sulfate. Equivalent to Q9CBA3|ML2279 putative transmembrane transport protein from Mycobacterium leprae (496 aa), FASTA scores: opt: 1637, E(): 1.8e-89, (59.15% identity in 487 aa overlap). Similar to various proteins (principally sulfate transporters) e.g. Q9X927|SCH5.25 putative integral membrane protein from Streptomyces coelicolor (830 aa), FASTA scores: opt: 1325, E(): 8e-71, (40.85% identity in 788 aa overlap); Q9I729|PA0103 probable sulfate transporter from Pseudomonas aeruginosa (523 aa), FASTA scores: opt: 1015, E(): 1.3e-52, (39.95% identity in 488 aa overlap); Q9KN88|VCA0077 sulfate permease family protein from Vibrio cholerae (553 aa), FASTA scores: opt: 629, E(): 9.6e-30, (30.95% identity in 423 aa overlap); etc. C-terminal part (aa 550-764) shows similarity to carbonic anhydrase e.g. P27134|CYNT_SYNP7 carbonic anhydrase (272 aa), FASTA scores: opt: 350, E(): 8.1e-15, (33.8% identity in 201 aa overlap). Contains PS00704 Prokaryotic-type carbonic anhydrases signature 1. Seems to belong to the SulP family. P96878 No intermediary metabolism and respiration P96878 P96878 GO:0004089,GO:0016021,GO:0055085,GO:0005215,GO:0008270,GO:0015976 4.2.1.1 P96878 NC_000962.3 Mycobrowser_v5 CDS 4145614 4146891 . - 0 Rv3703c Rv3703c Function unknown Conserved hypothetical protein Rv3703c, (MTV025.051c), len: 425 aa. Conserved hypothetical protein, similar to other hypothetical proteins e.g. Q9RD00|SCM1.44 from Streptomyces coelicolor (446 aa), FASTA scores: opt: 1480, E(): 1.4e-85, (53.9% identity in 421 aa overlap); P72841|SLR1303 from Synechocystis sp. strain PCC 6803 (410 aa), FASTA scores: opt: 533, E(): 4.5e-26, (36.6% identity in 429 aa overlap); Q9KYH7|SCC61A.16 from Streptomyces coelicolor (256 aa), FASTA scores: opt: 266, E(): 1.9e-09, (32.25% identity in 248 aa overlap); etc. Also similar to P95060|Rv0712|MTCY210.31 hypothetical 32.7 KDA protein from Mycobacterium tuberculosis (299 aa), FASTA scores: opt: 243, E(): 5.9e-08, (30.6% identity in 304 aa overlap). O69671 No conserved hypotheticals O69671 O69671 O69671 NC_000962.3 Mycobrowser_v5 CDS 3658114 3658638 . - 0 Rv3275c purE Involved in purine biosynthesis (sixth step). This subunit can alone transform air to CAIR, but in association with PURK, which possesses an ATPase activity, an enzyme complex is produced which is capable of converting air to CAIR efficiently under physiological condition [catalytic activity: 1-(5-phosphoribosyl)-5-amino-4-imidazole-carboxylate = 1-(5-phosphoribosyl)-5-aminoimidazole + CO(2)]. Probable phosphoribosylaminoimidazole carboxylase catalytic subunit PurE (air carboxylase) (AIRC) Rv3275c, (MTCY71.15c, PUR6), len: 174 aa. Probable purE, phosphoribosylaminoimidazole carboxylase catalytic subunit, equivalent to P46702|PUR6_MYCLE|pure|ML0736|B1308_F3_98 from Mycobacterium leprae (171 aa), FASTA scores: opt: 878, E(): 1.5e-43, (81.55% identity in 168 aa overlap). Also similar to others e.g. Q9AXD0|AIRC from Nicotiana tabacum (Common tobacco) (623 aa), FASTA scores: opt: 712, E(): 1.4e-33, (69.35% identity in 160 aa overlap) (similarity in C-terminal part for this one); Q44679|PUR6_CORAM from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (177 aa), FASTA scores: opt: 651, E(): 1.5e-30, (68.25% identity in 148 aa overlap); Q55498|PUR6_SYNY3|pure|SLL0901 from Synechocystis sp. strain PCC 6803 (176 aa), FASTA scores: opt: 639, E(): 7.1e-30, (60.5% identity in 167 aa overlap); etc. P9WHM1 No intermediary metabolism and respiration 3LP6 P96880 P9WHM1 GO:0004638,GO:0006189 4.1.1.21 P9WHM1 NC_000962.3 Mycobrowser_v5 CDS 3682110 3683024 . - 0 Rv3298c lpqC Function unknown; lipolytic enzyme involved in cellular metabolism. Possible esterase lipoprotein LpqC Rv3298c, (MTCY71.38c), len: 304 aa. Possible lpqC, esterase lipoprotein, equivalent to Q9CCL5|LPQC|ML0715 putative secreted hydrolase from Mycobacterium leprae (304 aa), FASTA scores: opt: 1543, E(): 1.3e-87, (71.6% identity in 303 aa overlap); and Q49658|B1308_F2_43 tubulin family protein from Mycobacterium leprae (302 aa), FASTA scores: opt: 1541, E(): 1.7e-87, (72.0% identity in 300 aa overlap). Also similar to Q9I5Z3|PA0543 hypothetical protein from Pseudomonas aeruginosa (322 aa), FASTA scores: opt: 439, E(): 8.9e-20, (32.3% identity in 319 aa overlap); Q9F2K9|SCH63.19c putative secreted protein from Streptomyces coelicolor (348 aa), FASTA scores: opt: 394, E(): 5.5e-17, (30.25% identity in 334 aa overlap); etc. And similar to O86367|LPQP|Rv0671|MTCI376.03c from Mycobacterium tuberculosis strain H37Rv (280 aa), FASTA scores: opt: 519, E(): 9.8e-25, (39.25% identity in 275 aa overlap). Probably lipoprotein, esterase membrane-bound, with 18 aa signal sequence as it contains appropriately positioned (PS00013) Prokaryotic membrane lipoprotein lipid attachment site. P96903 No cell wall and cell processes P96903 GO:0016787 3.1.-.- P96903 NC_000962.3 Mycobrowser_v5 CDS 3658635 3659924 . - 0 Rv3276c purK Involved in purine biosynthesis (sixth step). Possesses an ATPase activity that is dependent on the presence of air (aminoimidazole ribonucleotide). The association of PURK and pure produces an enzyme complex capable of converting air to CAIR efficiently under physiological condition [catalytic activity: 1-(5-phosphoribosyl)-5-amino-4-imidazole-carboxylate = 1-(5-phosphoribosyl)-5-aminoimidazole + CO(2)]. Probable phosphoribosylaminoimidazole carboxylase ATPase subunit PurK (air carboxylase) (AIRC) Rv3276c, (MTCY71.16c), len: 429 aa. Probable purK, phosphoribosylaminoimidazole carboxylase ATPase subunit , equivalent to P46701|PURK_MYCLE|ML0735|B1308_F1_32 phosphoribosylaminoimidazole carboxylase ATPase subunit from Mycobacterium leprae (439 aa), FASTA scores: opt: 2168, E(): 2.3e-123, (76.15% identity in 444 aa overlap). Also similar to others e.g. Q44678|PURK_CORAM from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (413 aa), FASTA scores: opt: 1179, E(): 9.1e-64, (48.35% identity in 389 aa overlap); Q9KZ85|PURK from Streptomyces coelicolor (368 aa), FASTA scores: opt: 1150, E(): 4.7e-62, (55.35% identity in 345 aa overlap); Q54975|PURK_SYNP7 from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (395 aa), FASTA scores: opt: 772, E(): 3e-39, (38.1% identity in 383 aa overlap); etc. Belongs to the PurK / PurT family. P9WHL9 No intermediary metabolism and respiration P65898 P9WHL9 GO:0005524,GO:0004638,GO:0006189 4.1.1.21 P9WHL9 NC_000962.3 Mycobrowser_v5 CDS 3661212 3662012 . - 0 Rv3279c birA BIRA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins [catalytic activity: ATP + biotin + APO-[acetyl-CoA:carbon-dioxide ligase (ADP forming)] = AMP + pyrophosphate + [acetyl-CoA:carbon-dioxide ligase (ADP forming)]]. Possible bifunctional protein BirA: biotin operon repressor + biotin--[acetyl-CoA-carboxylase] synthetase (biotin--protein ligase) Rv3279c, (MTCY71.19c), len: 266 aa. Possible birA, bifunctional protein: biotin operon repressor and biotin--[acetyl-CoA-carboxylase] synthetase, equivalent to Q9CCL3|BIRA|ML0732 biotin APO-protein ligase from Mycobacterium leprae (274 aa), FASTA scores: opt: 1189, E(): 2.3e-66, (71.2% identity in 271 aa overlap). But as it lacks a BirA h-t-h domain at N-terminus, may simply be biotin apo-protein ligase. Also similar to others e.g. Q9CNX6|BIRA|PM0296 from Pasteurella multocida (312 aa), FASTA scores: opt: 347, E(): 2.7e-14, (32.95% identity in 270 aa overlap); Q9HWC0|BIRA|PA4280 from Pseudomonas aeruginosa (312 aa), FASTA scores: opt: 335, E(): 1.5e-13, (34.2% identity in 272 aa overlap); Q9A6Z0|CC1936 from Caulobacter crescentus (250 aa), FASTA scores: opt: 332, E(): 1.9e-13, (33.6% identity in 238 aa overlap); P06709|BIRA_ECOLI (321 aa), FASTA scores: opt: 314, E(): 3.1e-12, (34.15% identity in 249 aa overlap); etc. Similar with other bacterial BIRA and with eukaryotic biotin APO-protein ligase. I6YFP0 No intermediary metabolism and respiration 2CGH,3L1A,3L2Z,3RUX P96884 I6YFP0 GO:0006464,GO:0004077 6.3.4.15 I6YFP0 NC_000962.3 Mycobrowser_v5 CDS 3664219 3664887 . + 0 Rv3282 Rv3282 Function unknown Conserved hypothetical protein Rv3282, (MTCY71.22), len: 222 aa. Conserved hypothetical protein, equivalent to Q49670|ML0729 1308R (hypothetical protein ML0729) from Mycobacterium leprae (213 aa), FASTA scores: opt: 945, E(): 5.5e-54, (68.55% identity in 213 aa overlap). Also similar to Q9EWV6|2SCK31.18 conserved hypothetical protein from Streptomyces coelicolor (206 aa), FASTA scores: opt: 459, E(): 1.3e-22, (47.35% identity in 209 aa overlap); P74331|MAF or SLL0905 MAF protein from Synechocystis sp. strain PCC 6803 (195 aa), FASTA scores: opt: 401, E(): 6.9e-19, (43.0% identity in 207 aa overlap); and shows weak similarity with various proteins e.g. Q9BUL6 acetylserotonin O-methyltransferase-like from Homo sapiens (Human) (621 aa), FASTA scores: opt: 282, E(): 8.9e-11, (31.6% identity in 193 aa overlap); O95671|ASMTL ASMTL protein from Homo sapiens (Human) (629 aa), FASTA scores: opt: 282, E(): 9e-11, (31.6% identity in 193 aa overlap); BAB51136|MLR4491 MAF protein from Rhizobium loti (Mesorhizobium loti) (199 aa), FASTA scores: opt: 269, E(): 2.3e-10, (29.3% identity in 198 aa overlap); etc. P9WK27 No conserved hypotheticals P96887 P9WK27 GO:0005737 P9WK27 NC_000962.3 Mycobrowser_v5 CDS 3708438 3709052 . - 0 Rv3322c Rv3322c Could cause methylation. Possible methyltransferase Rv3322c, (MTV016.22c), len: 204 aa. Conserved hypothetical protein, showing weak similarity to proteins including several methyltransferases e.g. Q9X9V1|ORF8 putative methyltransferase from Streptomyces coelicolor (208 aa), FASTA scores: opt: 193, E(): 1e-05, (36.35% identity in 132 aa overlap); and Q9XA90|SCF43A.25c putative methyltransferase from Streptomyces coelicolor (215 aa), FASTA scores: opt: 161, E(): 0.0014, (32.05% identity in 131 aa overlap); P74712|SLR1183 hypothetical 21.3 KDA protein from Synechocystis sp. strain PCC 6803 (194 aa), FASTA scores: opt: 155, E(): 0.0032, (27.35% identity in 150 aa overlap); Q9ABW8|CC0102 rRNA methyltransferase RSMB from Caulobacter crescentus (429 aa), FASTA scores: opt: 148, E(): 0.018, (31.5% identity in 162 aa overlap); etc. Also highly similar to O05796|Rv3120|MTCY164.30 hypothetical 21.8 KDA protein from Mycobacterium tuberculosis (200 aa), FASTA scores: opt: 691, E(): 1.2e-38, (56.5% identity in 200 aa overlap); and shows weak similarity to O69667|Rv3699|MTV025.047 putative methyltransferase from Mycobacterium tuberculosis (233 aa), FASTA scores: opt: 155, E(): 0.0037, (29.15% identity in 168 aa overlap). L7N687 No intermediary metabolism and respiration L7N687 GO:0008168 2.1.1.- L7N687 NC_000962.3 Mycobrowser_v5 CDS 3668169 3668954 . - 0 Rv3286c sigF The sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released. Thought to be involved in survival and proliferation in lung granulomas during infection. Thought to be involved in virulence and persistence processes. Modulates expression of the 16 KDa alpha-crystallin homologue/Rv2031c. Negatively regulated by Rv3287c|RSBW|USFX. Alternative RNA polymerase sigma factor SigF Rv3286c, (MTCY71.26), len: 261 aa. SigF, stress response/stationary phase RNA polymerase sigma factor (see citations below), similar to several Streptomyces RNA polymerase sigma factors e.g. Q9RPC8|sigh from Streptomyces coelicolor A3(2) (354 aa), FASTA scores: opt: 869, E(): 1.1e-45, (51.15% identity in 258 aa overlap); Q9RIT0|SIG1 from Streptomyces coelicolor (361 aa), FASTA scores: opt: 869, E(): 1.1e-45, (51.15% identity in 258 aa overlap); Q9ADM4|2SC10A7.38c from Streptomyces coelicolor (318 aa), FASTA scores: opt: 776, E(): 4.6e-40, (48.75% identity in 240 aa overlap); P37971|RPOF_STRCO|SIGF|RPOX|2SCD60.01c from Streptomyces coelicolor (287 aa), FASTA scores: opt: 717, E(): 1.6e-36, (44.5% identity in 245 aa overlap); P37970|RPOF_STRAU|SIGF|RPOX from Streptomyces aureofaciens (297 aa); etc. Contains possible helix-turn-helix motif at aa 229-250 (+7.38 SD). Similar to the sigma-70 factor family. Seems expressed in stationary phase and under stress conditions in vitro (see citations below). P9WGI3 No information pathways Q7D5S2 P9WGI3 GO:0016987,GO:0003700,GO:0006352,GO:0006355 P9WGI3 NC_000962.3 Mycobrowser_v5 CDS 3670445 3671794 . - 0 Rv3290c lat Possibly involved in L-alpha-aminoadipic acid (L-AAA) biosynthesis. Catalyzes the transfer of the terminal amino group of L-lysine or L-ornithine to alpha-ketoglutarate [catalytic activity: L-lysine + 2-oxoglutarate = 2-aminoadipate 6-semialdehyde + L-glutamate]. Probable L-lysine-epsilon aminotransferase Lat (L-lysine aminotransferase) (lysine 6-aminotransferase) Rv3290c, (MTCY71.30), len: 449 aa. Probable lat, lysine-epsilon aminotransferase, similar to Q05174|LAT_NOCLA from Nocardia lactamdurans (450 aa), FASTA scores: opt: 1702, E(): 1.1e-99, (60.35% identity in 439 aa overlap); and Q01767|Q53823|LAT_STRCL from Streptomyces clavuligerus (457 aa), FASTA scores: opt: 1676, E(): 4.9e-98, (60.15% identity in 434 aa overlap). Also some similarity to 4-aminobutyrate aminotransferase proteins (gamma-amino-N-butyrate transaminases). Belongs to class-III of pyridoxal-phosphate-dependent aminotransferases. Cofactor: pyridoxal phosphate. P9WQ77 No intermediary metabolism and respiration 2CIN,2CJD,2CJG,2CJH,2JJE,2JJF,2JJG,2JJH P63509 P9WQ77 GO:0017000,GO:0030170,GO:0045484 2.6.1.36 P9WQ77 NC_000962.3 Mycobrowser_v5 CDS 3673602 3675086 . + 0 Rv3293 pcd Involved in L-alpha-aminoadipic acid (L-AAA) biosynthesis (in the second step; the first step is promoted by lat enzyme. Probable piperideine-6-carboxilic acid dehydrogenase Pcd (piperideine-6-carboxylate dehydrogenase) Rv3293, (MTCY71.33), len: 494 aa. Probable pcd, piperideine-6-carboxylic acid dehydrogenase, highly similar to others e.g. O85725|PCD semialdehyde dehydrogenase from Streptomyces clavuligerus (512 aa), FASTA scores: opt: 2214, E(): 6.7e-121, (68.75% identity in 496 aa overlap) (see Alexander & Jensen 1998); Q9I4U7|PA1027 probable aldehyde dehydrogenase from Pseudomonas aeruginosa (529 aa), FASTA scores: opt: 1984, E(): 1.4e-107, (64.5% identity in 493 aa overlap); BAB49892|MLL2867 aldehyde dehydrogenase from Rhizobium loti (Mesorhizobium loti) (504 aa), FASTA scores: opt: 1964, E(): 2e-106, (62.8% identity in 476 aa overlap); Q9A8Y1|CC1216 aldehyde dehydrogenase from Caulobacter crescentus (507 aa), FASTA scores: opt: 1909, E(): 3.1e-103, (59.95% identity in 497 aa overlap); O54199|PCD piperideine-6-carboxilic acid dehydrogenase from Streptomyces clavuligerus (496 aa), FASTA scores: opt: 1748, E(): 6.4e-94, (60.6% identity in 467 aa overlap); and Q9F1U8|PCD piperideine-6-carboxylate dehydrogenase from 'Flavobacterium' lutescens (510 aa), FASTA scores: opt: 1656, E(): 1.4e-88, (54.05% identity in 481 aa overlap) (see Fujii et al., 2000); etc. Contains PS00687 Aldehyde dehydrogenases glutamic acid active site. Note that ORF Rv3290c seems to encoded the putative lat enzyme. Note that previously known as aldB. L7N650 No intermediary metabolism and respiration Q7D5R7 L7N650 GO:0016491,GO:0055114 1.5.-.- L7N650 NC_000962.3 Mycobrowser_v5 CDS 3676066 3676731 . + 0 Rv3295 Rv3295 Involved in transcriptional mechanism. Probable transcriptional regulatory protein (probably TetR-family) Rv3295, (MTCY71.35), len: 221 aa. Probable transcriptional regulator TetR-family, equivalent to Q9CCL4|ML0717 putative TetR-family transcriptional regulator from Mycobacterium leprae (223 aa), FASTA scores: opt: 1260, E(): 7.2e-75, (85.45% identity in 220 aa overlap). Also highly similar to other streptomyces regulators e.g. Q9RD77|SCF43.11 from Streptomyces coelicolor (205 aa), FASTA scores: opt: 442, E(): 9.8e-22, (38.6% identity in 202 aa overlap); Q9RKY8|SC6D7.09 from Streptomyces coelicolor (220 aa), FASTA scores: opt: 215, E(): 5.9e-07, (31.85% identity in 135 aa overlap); Q9L0U5|SCD35.06 from Streptomyces coelicolor (240 aa), FASTA scores: opt: 214, E(): 7.4e-07, (28.2% identity in 156 aa overlap); etc. Similar to the TetR/AcrR family of transcriptional regulators. Contains potential helix-turn-helix motif at aa 33-54 (+4.42 SD). P96900 No regulatory proteins P96900 P96900 GO:0006350,GO:0003700,GO:0006355 P96900 NC_000962.3 Mycobrowser_v5 CDS 3687685 3689442 . - 0 Rv3302c glpD2 Involved in aerobic respiration and oxidation of glycerol. Reduces an acceptor and generates glycerone phosphate from Sn-glycerol 3-phosphate. Possibly play a role in metabolism of riboflavin, FAD,FMN [catalytic activity: SN-glycerol 3-phosphate + acceptor = glycerone phosphate + reduced acceptor]. Probable glycerol-3-phosphate dehydrogenase GlpD2 Rv3302c, (MTCI418A.04c, MTV016.01c), len: 585 aa. Probable glpd2, glycerol-3-phosphate dehydrogenase, equivalent to P53435|GLPD_MYCLE|ML0713|L308_C1_179 glycerol-3-phosphate dehydrogenase from Mycobacterium leprae (585 aa), FASTA scores: opt: 3489, E(): 2.2e-198, (90.75% identity in 584 aa overlap). Also highly similar to many e.g. Q9L0I3|SCD63.06 from Streptomyces coelicolor (568 aa), FASTA scores: opt: 2203, E(): 1.6e-122, (59.95% identity in 564 aa overlap); Q9RVK8|DR1019 from Deinococcus radiodurans (522 aa), FASTA scores: opt: 949, E(): 1.4e-48, (37.0% identity in 538 aa overlap); BAB53412|MLR7270 from Rhizobium loti (Mesorhizobium loti) (505 aa), FASTA scores: opt: 861, E(): 2.2e-43, (37.3% identity in 488 aa overlap); P18158|GLPD_BACSU from B. subtilis (555 aa), FASTA scores: opt: 768, E(): 7.2e-38, (32.85% identity in 484 aa overlap); etc. Also similar to Mycobacterium tuberculosis protein Q10502|GLPD_MYCTU|MTCY427_31c|Rv2249c glycerol-3-phosphate dehydrogenase (516 aa), FASTA scores: opt: 843, E(): 2.6e-42, (36.5% identity in 515 aa overlap). Contains PS00978 FAD-dependent glycerol-3-phosphate dehydrogenase signature 2. Cofactor: FAD (by similarity). Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family. P9WN79 No intermediary metabolism and respiration P64184 P9WN79 GO:0009331,GO:0006072,GO:0055114,GO:0004368 1.1.5.3 P9WN79 NC_000962.3 Mycobrowser_v5 CDS 3689457 3690938 . - 0 Rv3303c lpdA Involved in energy metabolism. Can catalyze the reduction of electron acceptors such as 2,6-dimethyl-1,4-benzoquinone (DMBQ) and 5-hydroxy-1,4-napthaquinone (5-HNQ) [catalytic activity: NAD(P)H + a quinone + H+ -> a quinol + NAD(P)+]. NAD(P)H quinone reductase LpdA Rv3303c, (MTV016.02c), len: 493 aa. Probable lpdA, quinone reductase, similar to e.g. Q9EWV3|2SCK31.22c putative oxidoreductase from Streptomyces coelicolor (475 aa), FASTA scores: opt: 1420, E(): 2.4e-77, (54.9% identity in 471 aa overlap); Q9A7J2|CC1731 lipoamide dehydrogenase (E3 component,pyruvate dehydrogenase complex) from Caulobacter crescentus (466 aa), FASTA scores: opt: 696, E(): 3.6e-34, (29.6% identity in 463 aa overlap); Q04829|LPD|DLDH_HALVO dihydrolipoamide dehydrogenase from Halobacterium volcanii (Haloferax volcanii) (474 aa), FASTA scores: opt: 675, E(): 6.5e-33, (29.3% identity in 471 aa overlap); P50970|DLDH_ZYMMO|LPD dihydrolipoamide dehydrogenase from Zymomonas mobilis, FASTA scores: opt: 658, E(): 6.6e-32, (30.4% identity in 464 aa overlap); etc. Belongs to the pyridine nucleotide-disulfide oxidoreductases class-I. Cofactor: FAD. P9WHH7 No intermediary metabolism and respiration 1XDI O53355 P9WHH7 GO:0003955,GO:0045454,GO:0005737,GO:0004148,GO:0055114,GO:0050660 1.6.5.2 P9WHH7 NC_000962.3 Mycobrowser_v5 CDS 3691141 3691620 . + 0 Rv3304 Rv3304 Function unknown Conserved protein Rv3304, (MTV016.03), len: 159 aa. Conserved protein, very similar to Q9CCL6|ML0711 hypothetical protein from Mycobacterium leprae (159 aa), FASTA scores: opt: 1041, E(): 6.1e-62, (91.8% identity in 159 aa overlap); and Q49927|L308_F3_97 from M. leprae (174 aa), FASTA scores: opt: 974, E(): 1.8e-57, (91.2% identity in 149 aa overlap). Also highly similar to Q9AD81|SCK13.10c conserved hypothetical protein from Streptomyces coelicolor (145 aa), FASTA scores: opt: 615, E(): 7.8e-34, (60.55% identity in 147 aa overlap); and shows some similarity to other various hypotheticals proteins. ORF continues upstream with possible start at 2198 (equivalent to AAK47746 from Mycobacterium tuberculosis strain CDC1551 (212 aa) but shorter 53 aa). O53356 No conserved hypotheticals O53356 O53356 O53356 NC_000962.3 Mycobrowser_v5 CDS 3692805 3693989 . - 0 Rv3306c amiB1 Involved in cellular metabolism, active on carbon aliphatic amides and/or on many aromatic amides [catalytic activity: a monocarboxylic acid amide + H(2)O = a monocarboxylate + NH(3)]. Probable amidohydrolase AmiB1 (aminohydrolase) Rv3306c, (MTV016.05c), len: 394 aa. Probable amiB1, aminohydrolase, similar to several belonging to peptidase family M40 (and to hypothetical proteins) e.g. P54983|AMHX_BACSU amidohydrolase AMHX from Bacillus subtilis (389 aa), FASTA scores: opt: 286, E(): 9.9e-10, (26.6% identity in 351 aa overlap); P76052|ABGB_ECOLI Aminobenzoyl-glutamate utilizatio from Escherichia coli (481 aa), FASTA scores: opt: 383, E(): 2.1e-15, (30.5% identity in 328 aa overlap); P44765|YDAJ_HAEIN hypothetical protein HI0584 from Haemophilus influenzae (423 aa), FASTA scores: opt: 297, E(): 2.4e-10, (29.6% identity in 274 aa overlap). Note that previously known as amiB. L7N690 No intermediary metabolism and respiration Q7D5Q9 L7N690 GO:0008152,GO:0005515,GO:0016787 3.5.1.- L7N690 NC_000962.3 Mycobrowser_v5 CDS 3694054 3694860 . + 0 Rv3307 deoD Involved in purine nucleoside salvage. Cleavage of guanosine or inosine to respective BASES and sugar-1-phosphate molecules [catalytic activity: purine nucleoside + orthophosphate = purine + alpha-D-ribose 1-phosphate]. Probable purine nucleoside phosphorylase DeoD (inosine phosphorylase) (PNP) Rv3307, (MTV016.06), len: 268 aa. Probable deoD (alternate gene name: punA), purine nucleoside phosphorylase, similar to others especially P46862|PUNA_MYCLE|DEOD_MYCLE|ML0707|L308_F2_56 from M. leprae (268 aa), FASTA scores: opt: 1373, E(): 1.5e-74, (82.05% identity in 262 aa overlap); Q9EWV2|2SCK31.24 from Streptomyces coelicolor (274 aa), FASTA scores: opt: 1026, E(): 6.4e-54, (60.5% identity in 266 aa overlap); P81989|PUNA_CELSP from Cellulomonas sp (282 aa), FASTA scores: opt: 963, E(): 3.6e-50, (58.9% identity in 270 aa overlap); Q9X1T2|TM1596 from Thermotoga maritima (265 aa), FASTA scores: opt: 584, E(): 1.1e-27, (39.55% identity in 263 aa overlap); etc. Belongs to the PNP/MTAP family 2 of phosphorylases. P9WP01 No intermediary metabolism and respiration 1G2O,1I80,1N3I,3IOM P0A538 P9WP01 GO:0004731,GO:0009116 2.4.2.1 P9WP01 NC_000962.3 Mycobrowser_v5 CDS 3696470 3697093 . - 0 Rv3309c upp Involved in pyrimidine salvage pathway [catalytic activity: UMP + pyrophosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate]. Probable uracil phosphoribosyltransferase Upp (UMP pyrophosphorylase) (uprtase) (UMP diphosphorylase) Rv3309c, (MTV016.08c), len: 207 aa. Probable upp, uracil phosphoribosyltransferase, identical to P94928|UPP uracil phosphoribosyltransferase from Mycobacterium bovis (207 aa). Also similar to others e.g. P36399|UPP_STRSL from Streptococcus salivarius (209 aa), FASTA scores: opt: 658, E(): 4.7e-35, (48.3% identity in 207 aa overlap); Q9A194|UPP|SPY0392 from Streptococcus pyogenes (209 aa), FASTA scores: opt:650, E(): 1.5e-34, (47.35% identity in 207 aa overlap); Q9RE01|UPP from Lactobacillus plantarum (209 aa), FASTA scores: opt: 644, E(): 3.7e-34, (46.4% identity in 207 aa overlap); etc. Belongs to the uprtase family. P9WFF3 No intermediary metabolism and respiration P0A658 P9WFF3 GO:0004845,GO:0006223,GO:0009116 2.4.2.9 P9WFF3 NC_000962.3 Mycobrowser_v5 CDS 3698121 3699383 . + 0 Rv3311 Rv3311 Function unknown Conserved protein Rv3311, (MTV016.10), len: 420 aa. Conserved protein, equivalent to Mycobacterium leprae hypothetical proteins Q9CCL8|ML0703 (423 aa), FASTA scores: opt: 2185, E(): 5.5e-120, (77.55% identity in 423 aa overlap); Q49918|L308_F2_61 (167 aa), FASTA scores: opt: 929, E(): 3.5e-47, (84.4% identity in 167 aa overlap) (similarity at C-terminus for this one); and Q49914|L308_F1_17 (166 aa), FASTA scores: opt: 900, E(): 1.7e-45, (79.0% identity in 162 aa overlap) (similarity at N-terminus for this one); Q49923|U0308N (86 aa) FASTA scores: opt: 149, E(): 0.052, (48.35% identity in 60 aa overlap); etc. Note that the Rv3311 corresponding protein in Mycobacterium leprae is similar to products of two adjacent ORFs. Also some similarity to Q9XI61|F9L1.1 hypothetical protein from Arabidopsis thaliana (Mouse-ear cress) (523 aa), FASTA scores: opt: 134, E(): 1.8, (25.1% identity in 203 aa overlap). Equivalent to AAK47753 from Mycobacterium tuberculosis strain CDC1551 (431 aa) but shorter 12 aa. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). O53362 No conserved hypotheticals O53362 NC_000962.3 Mycobrowser_v5 CDS 3703464 3703865 . - 0 Rv3315c cdd This enzyme scavenge exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis [catalytic activity: cytidine + H(2)O = uridine + NH(3)]. Probable cytidine deaminase Cdd (cytidine aminohydrolase) (cytidine nucleoside deaminase) Rv3315c, (MTV016.15c), len: 133 aa. Probable cdd, cytidine deaminase, equivalent to Q9CBD3|CDD|ML2174 cytidine deaminase from Mycobacterium leprae (134 aa), FASTA scores: opt: 516, E(): 5.8e-28, (56.8% identity in 132 aa overlap). Also highly similar to many e.g. Q9AK37|2SCK8.15 from Streptomyces coelicolor (130 aa), FASTA scores: opt: 523, E(): 1.9e-28, (60.0% identity in 130 aa overlap); Q9KD53|CDD|BH1366 from Bacillus halodurans (132 aa), FASTA scores: opt: 305, E(): 9.2e-14, (41.55% identity in 130 aa overlap); P56389|CDD_MOUSE|CDA|CDD from Mus musculus (Mouse) (146 aa), FASTA scores: opt: 287, E(): 1.6e-12, (40.3% identity in 124 aa overlap); P19079|CDD_BACSU (136 aa), FASTA scores: opt: 270, E(): 2.1e-11, (28.6% identity in 127 aa overlap); etc. Contains PS00903 Cytidine and deoxycytidylate deaminases zinc-binding region signature. Belongs to the cytidine and deoxycytidylate deaminases family. Cofactor: zinc (by similarity). P9WPH3 No intermediary metabolism and respiration 3IJF O53367 P9WPH3 GO:0046087,GO:0008270,GO:0004126 3.5.4.5 P9WPH3 NC_000962.3 Mycobrowser_v5 CDS 3767346 3768140 . - 0 Rv3351c Rv3351c Function unknown Conserved hypothetical protein Rv3351c, (MTV004.08c), len: 264 aa. Hypothetical protein, highly similar to C-terminal region (aa 292-479) of O53608|Rv0063|MTV030.06 oxidoreductase from Mycobacterium tuberculosis (479 aa), FASTA scores: opt: 699, E(): 1.7e-36, (54.75% identity in 190 aa overlap). Shows some similarity to Q9KYD6|SCD72A.20 putative lipoprotein (fragment) from Streptomyces coelicolor (403 aa), FASTA scores: opt: 192, E(): 9.1e-05, (27.9% identity in 154 aa overlap); and P71091|YGAK hypothetical 54.4 KDA protein from Bacillus subtilis (480 aa), FASTA scores: opt: 174, E(): 0.0014, (26.5% identity in 166 aa overlap). Note that the two upstream ORFs Rv3352c and Rv3353c also show similarity to Rv0063 (MTV030_7). Sequence was checked but no errors found. Predicted to be an outer membrane protein (See Song et al., 2008). O50380 No conserved hypotheticals O50380 O50380 GO:0016491,GO:0050660 O50380 NC_000962.3 Mycobrowser_v5 CDS 3704102 3704440 . + 0 Rv3316 sdhC Involved in tricarboxylic acid cycle. Mono-heme cytochrome of the succinate dehydrogenase complex. Probable succinate dehydrogenase (cytochrome B-556 subunit) SdhC (succinic dehydrogenase) (fumarate reductase) (fumarate dehydrogenase) (fumaric hydrogenase) Rv3316, (MTV016.16), len: 112 aa. Probable sdhC, cytochrome B-556 of succinate dehydrogenase SdhC subunit , transmembrane protein, equivalent (but shorter 35 aa) to Q9CCM0|SDHC|ML0699 putative succinate dehydrogenase cytochrome B-556 subunit from Mycobacterium leprae (153 aa), FASTA scores: opt: 692, E(): 1.2e-39, (88.4% identity in 112 aa overlap). Also similar to others e.g. Q9KZ88|SC5G8.26c from Streptomyces coelicolor (126 aa), FASTA scores: opt: 484, E(): 8.3e-26, (65.65% identity in 99 aa overlap); Q9RVR8|DR0954 from Deinococcus radiodurans (118 aa), FASTA scores: opt: 195, E(): 1.7e-06, (36.8% identity in 87 aa overlap); Q9HQ63|DHSD_HALN1|SDHD|SDHC|VNG1310G from Halobacterium sp. strain NRC-1 (130 aa), FASTA scores: opt: 192, E(): 2.9e-06, (37.85% identity in 74 aa overlap); P72109|DHSD_NATPH|SDHD|SDHC from Natronomonas pharaonis (Natronobacterium pharaonis) (130 aa), FASTA scores: opt: 183, E(): 1.1e-05, (35.15% identity in 74 aa overlap); etc. Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, cytochrome B-556, and an hydrophobic anchor protein. Belongs to the cytochrome B560 family. O53368 No intermediary metabolism and respiration O53368 O53368 GO:0000104,GO:0055114 1.3.99.1 O53368 NC_000962.3 Mycobrowser_v5 CDS 3713394 3714332 . - 0 Rv3328c sigJ The sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released. Probable alternative RNA polymerase sigma factor (fragment) SigJ Rv3328c, (MTV016.28c), len: 312 aa. Probable sigJ, alternative RNA polymerase sigma factor (see citations below), highly similar to many e.g. Q9K3H7|2SCG18.10c from Streptomyces coelicolor (295 aa), FASTA scores: opt: 642, E(): 7.3e-31, (42.8% identity in 292 aa overlap); Q9A3D8|CC3266 from Caulobacter crescentus (291 aa), FASTA scores: opt: 607, E(): 8.4e-29, (39.8% identity in 294 aa overlap); Q9RD74|SCF43.14c from Streptomyces coelicolor (324 aa), FASTA scores: opt: 555, E(): 1.1e-25, (41.1% identity in 297 aa overlap); etc. Similar also to U00022_20 from Mycobacterium leprae; and MTCI28_22 and MSU87307_1. Also similar to O50445|SIGI|Rv1189|MTV005.25|MTCI364.01 putative RNA polymerase sigma factor from Mycobacterium tuberculosis (290 aa), FASTA scores: opt: 426, E(): 4.2e-18, (32.65% identity in 294 aa overlap). Equivalent to AAK47774 from Mycobacterium tuberculosis strain CDC1551 (282 aa) but longer 30 aa. Contains probable helix-turn-helix motif at aa 129-150 (Score 1126, +3.02 SD). Belongs to the sigma-70 factor family, ECF subfamily. L0TCG5 No information pathways O53378 L0TCG5 GO:0016987,GO:0003700,GO:0006352,GO:0006355 L0TCG5 NC_000962.3 Mycobrowser_v5 CDS 3724615 3725844 . - 0 Rv3339c icd1 Involved in the KREBS cycle [catalytic activity: isocitrate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH]. Probable isocitrate dehydrogenase [NADP] Icd1 (oxalosuccinate decarboxylase) (IDH) (NADP+-specific ICDH) (IDP) Rv3339c, (MTV016.39c), len: 409 aa. Probable icd1, isocitrate dehydrogenase NADP-dependent, highly similar to many e.g. Q9A5C8|CC2522 from Caulobacter crescentus (403 aa), FASTA scores: opt: 1972, E(): 4.6e-115, (72.45% identity in 403 aa overlap); AAF73472|ICD from Rhizobium meliloti (404 aa), FASTA scores: opt: 1968, E(): 8.1e-115, (73.2% identity in 403 aa overlap); P50215|IDH_SPHYA from Sphingomonas yanoikuyae (406 aa), FASTA scores: opt: 1964, E(): 1.4e-114, (71.45% identity in 403 aa overlap); etc. Contains PS00470 Isocitrate and isopropylmalate dehydrogenases signature. Belongs to the isocitrate and isopropylmalate dehydrogenases family. Note that in H37Rv, Rv0066c is named icd2 and Rv3339c is icd1 while in CDC1551 and Erdman strains, Rv0066c is icd1 and Rv3339c is icd2. P9WKL1 No intermediary metabolism and respiration P65097 P9WKL1 GO:0006097,GO:0004450,GO:0006102,GO:0000287,GO:0055114,GO:0006099,GO:0051287 1.1.1.42 P9WKL1 NC_000962.3 Mycobrowser_v5 CDS 3726127 3727476 . + 0 Rv3340 metC Transforms O-acetylhomoserine into L-methionine [catalytic activity: O-acetyl-L-homoserine + methanethiol = L-methionine + acetate]. Probable O-acetylhomoserine sulfhydrylase MetC (homocysteine synthase) (O-acetylhomoserine (thiol)-lyase) (OAH sulfhydrylase) (O-acetyl-L-homoserine sulfhydrylase) Rv3340, (MTV016.40), len: 449 aa. Probable metC, O-acetyl-L-homoserine sulfhydrylase, highly similar to many e.g. Q9K9P2|BH2603 O-acetylhomoserine sulfhydrylase from Bacillus halodurans (430 aa), FASTA scores: opt: 1716, E(): 3.3e-97, (60.45% identity in 425 aa overlap); Q9HUE4|METY|PA5025 homocysteine synthase from Pseudomonas aeruginosa (425 aa), FASTA scores: opt: 1517, E(): 4.4e-85, (56.95% identity in 425 aa overlap); Q9WZY4|TM0882 O-acetylhomoserine sulfhydrylase from Thermotoga maritima (430 aa), FASTA scores: opt: 1488, E(): 2.6e-83, (55.75% identity in 418 aa overlap); BAB54344|MLR8465 O-acetylhomoserine sulfhydrylase from Rhizobium loti (Mesorhizobium loti) (426 aa), FASTA scores: opt: 1445, E(): 1.1e-80, (53.2% identity in 419 aa overlap); P50125|CYSD_EMENI O-acetylhomoserine (thiol)-lyase from Emericella nidulans (Aspergillus nidulans) (437 aa), FASTA scores: opt: 1442, E(): 1.7e-80, (53.7% identity in 430 aa overlap); etc. Contains PS00868 Cys/Met metabolism enzymes pyridoxal-phosphate attachment site. Cofactor: pyridoxal phosphate. Belongs to the trans-sulfuration enzymes family. O53390 No intermediary metabolism and respiration O53390 O53390 GO:0006520,GO:0016829,GO:0030170,GO:0003961 2.5.1.49 O53390 NC_000962.3 Mycobrowser_v5 tRNA 3348659 3348730 . - 0 MTB000040 glnU - tRNA-Gln (CTG) glnU, tRNA-Gln; anticodon ctg, length = 72 No stable RNAs NC_000962.3 Mycobrowser_v5 CDS 3769804 3770649 . - 0 Rv3356c folD Necessary for the biosynthesis of purines, thymydylate, methionine, histidine, pantothenate, and formyl tRNA-met [catalytic activity: 5,10-methylenetetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH] [catalytic activity: 5,10-methenyltetrahydrofolate + H(2)O = 10-formyltetrahydrofolate]. Probable bifunctional protein FolD: methylenetetrahydrofolate dehydrogenase + methenyltetrahydrofolate cyclohydrolase Rv3356c, (MTV004.13c), len: 281 aa. Probable folD, bifunctional enzyme include methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase , equivalent to O32879|fold|ML0674 methylenetetrahydrofolate dehydrogenase (putative methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase) from Mycobacterium leprae (282 aa), FASTA scores: opt: 1624, E(): 1.2e-93, (86.45% identity in 281 aa overlap). Also similar to many others e.g. Q9K3J6|fold from Streptomyces coelicolor (284 aa), FASTA scores: opt: 1223, E(): 9.5e-69, (66.65% identity in 279 aa overlap); Q9K966|fold from Bacillus halodurans (279 aa), FASTA scores: opt: 886, E(): 7.7e-48, (47.15% identity in 280 aa overlap); P54382|FOLD_BACSU from Bacillus subtilis (283 aa), FASTA scores: opt: 820, E(): 9.7e-44, (45.7% identity in 280 aa overlap); P51696|FOLD_PHOPO from Photobacterium phosphoreum (285 aa), FASTA scores: opt: 778, E(): 4e-41, (44.9% identity in 283 aa overlap); P24186|FOLD_ECOLI|ads|B0529 from Escherichia coli (287 aa), FASTA scores: opt: 741, E(): 0,44.4, (44.4% identity in 277 aa overlap); etc. Also highly similar to MLCB1779_9 from Mycobacterium leprae cosmid B1779 (282 aa) (86.5% identity in 281 aa overlap). Similar to other dehydrogenase/cyclohydrolase enzymes or domains. P9WG81 No intermediary metabolism and respiration 2C2X,2C2Y O50385 P9WG81 GO:0009396,GO:0000105,GO:0004477,GO:0009086,GO:0004488,GO:0006730,GO:0055114,GO:0006164 3.5.4.9,1.5.1.5 P9WG81 NC_000962.3 Mycobrowser_v5 CDS 3771344 3772534 . + 0 Rv3359 Rv3359 Function unknown; probably involved in cellular metabolism. Possible oxidoreductase Rv3359, (MTV004.16), len: 396 aa. Possible oxidoreductase, similar to N-terminal part of various proteins (hypothetical unknowns or oxidoreductases) e.g. Q9ZB94 hypothetical 69.3 KDA protein from Rhodococcus erythropolis (649 aa), FASTA scores: opt: 509, E(): 3e-24, (30.0% identity in 380 aa overlap); O29991|AF0248 NADH-dependent flavin oxidoreductase from Archaeoglobus fulgidus (378 aa), FASTA scores: opt: 478, E(): 1.6e-22, (32.45% identity in 379 aa overlap); Q9HUH9|PA4986 probable oxidoreductase from Pseudomonas aeruginosa (648 aa), FASTA scores: opt: 412, E(): 3.3e-18, (30.45% identity in 384 aa overlap); Q9KCT8|BH1481 NADH oxidase from Bacillus halodurans (338 aa), FASTA scores: opt: 404, E(): 6.1e-18, (30.2% identity in 275 aa overlap); etc. Some weak similarity to Mycobacterium leprae MLCB1779_10. O50388 No intermediary metabolism and respiration O50388 O50388 GO:0055114,GO:0016491,GO:0010181 1.-.-.- O50388 NC_000962.3 Mycobrowser_v5 CDS 3793257 3794867 . - 0 Rv3379c dxs2 Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP). Possibly involved in deoxyxylulose-5-phosphate pathway (DXP) of isoprenoid biosynthesis (at the first step), and biosynthetic pathway to thiamine andpyridoxol (at the first step). Probable 1-deoxy-D-xylulose 5-phosphate synthase Dxs2 (1-deoxyxylulose-5-phosphate synthase) (DXP synthase) (DXPS) Rv3379c, (MTV004.37c), len: 536 aa. Probable dxs2, 1-deoxy-D-xylulose 5-phosphate synthase, similar to many e.g. Q9F1V2|DXS from Kitasatospora griseola (Streptomyces griseolosporeus) (649 aa), FASTA scores: opt: 1274, E(): 5.4e-71, (50.9% identity in 570 aa overlap); Q9X7W3|DXS_STRCO|SC6A5.17 from Streptomyces coelicolor (656 aa), FASTA scores: opt: 1248, E(): 2.2e-69, (50.55% identity in 568 aa overlap); Q9RBN6|DXS_STRC1 from Streptomyces sp. strain CL190 (631 aa), FASTA scores: opt: 1237, E(): 1e-68, (49.1% identity in 570 aa overlap); Q50000|DXS_MYCLE|TKTB|ML1038 from Mycobacterium leprae (643 aa), FASTA scores: opt: 1215, E(): 2.4e-67, (46.75% identity in 571 aa overlap); Q9R6S7|DXS_SYNLE from Synechococcus leopoliensis (636 aa), FASTA scores: opt: 849, E(): 8.9e-45, (38.55% identity in 550 aa overlap); etc. Also similar to O07184|DXS_MYCTU|Rv2682c|MT2756|MTCY05A6.03c from Mycobacterium tuberculosis (638 aa), FASTA scores: opt: 1226, E(): 4.9e-68, (48.9% identity in 558 aa overlap). Belongs to the transketolase family, DXS subfamily. Cofactor: thiamine pyrophosphate (by similarity). Note that the N-terminus of this putative protein appears to have been interrupted by the adjacent IS6110 element. O50408 No intermediary metabolism and respiration O50408 O50408 GO:0016740,GO:0008152 2.2.-.- O50408 NC_000962.3 Mycobrowser_v5 CDS 3777737 3778201 . + 0 Rv3366 spoU Causes methylation Probable tRNA/rRNA methylase SpoU (tRNA/rRNA methyltransferase) Rv3366, (MTV004.23), len: 154 aa. Probable spoU, tRNA/rRNA methylase, equivalent to Q9CCU7|ML0419 putative tRNA/rRNA methyltransferase from Mycobacterium leprae (158 aa), FASTA scores: opt: 861, E(): 1.2e-50, (83.75% identity in 154 aa overlap); and O07698|MLCL383.24c rRNA methylase from Mycobacterium leprae (169 aa), F