Gene ML0300
in Mycobacterium leprae TN
General annotation
| Type | CDS |
| Function | Involved in thiamine biosynthesis. Condenses 4-methy-(beta-hydroxyethyl)-thiazole monophosphate (THZ-P) and 4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [Catalytic activity: 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-4-methyl-5-(2-phosphonooxyethyl)-thiazole = diphosphate + thiamine monophosphate]. |
| Product | Probable thiamine-phosphate pyrophosphorylase ThiE (TMP pyrophosphorylase) (TMP-PPase) (Thiamine-phosphate synthase). |
| Comments | ML0300, len: 235 aa. Probable thiE, thiamin phosphate pyrophosphorylase (EC 2.5.1.3), highly similar to Rv0414c|THIE_MYCTU|P96260 probable thiE, thiamin phosphate pyrophosphorylase from M. tuberculosis (222 aa), Fasta scores: E(): 0, (78.9% identity in 223 aa overlap). Similar to many other e.g. THIE_ECOLI|P30137 thiE, thiamine-phosphate pyrophosphorylase from Escherichia coli (211 aa), Fasta scores: E(): 5e-10, (38.7% identity in 199 aa overlap). Previously sequenced as THIE_MYCLE|Q9ZBL5 (235 aa), Fasta scores: E(): 0, (99.6% identity in 235 aa overlap). Belongs to the TMP-PPase family. |
| Functional category | Intermediary metabolism and respiration |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 382835 | 383542 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium leprae TN|ML0300|thiE
VQEPHRQPAICLSTRLAKARLYLCTDARRERGDLAQFVNAALAGGVDIVQLRDKGSVGEQQFGPLEARDALAACEIFTDATGRHDALFAVNDRADIARAAGADVLHLGQGDLPPGVARQIVCRQMLIGLSTHDRHQVAAAVAALDAGLVDYFCVGPCWPTPTKPDRPAPGLELVRAAAELAGDKPWFAIGGIDAQRLPDVLHAGARRIVVVRAITAAADPRAAAEQLISTLTATS
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