Gene ML0548 in Mycobacterium leprae TN
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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
Gene summary information
Gene namepriA
Gene length1956 bp
IdentifierML0548
Location 664261 bp
Protein summary information
Molecular mass69923.2 Da
Isoelectric point9.63
Protein length652 amino acids
Orthologs
M. tuberculosis H37RvRv1402
M. lepromatosis Mx1-22AMLPM_0548
Cross-references
UniProtQ9CCQ3
Gene OntologyDna binding, Dna replication, synthesis of rna primer, Alpha dna polymerase:primase complex, Helicase activity, Zinc ion binding, Atp binding
Enzyme Classification3.6.1.-
Precomputed results
BLASTReport
General annotation
TypeCDS
FunctionRECOGNIZES A SPECIFIC HAIRPIN SEQUENCE ON PHIX SSDNA; THIS STRUCTURE IS THEN RECOGNIZED AND BOUND BY PROTEINS PRIB AND PRIC. FORMATION OF THE PRIMOSOME PROCEEDS WITH THE SUBSEQUENT ACTIONS OF DNAB, DNAC, DNAT AND PRIMASE. PRIA THEN FUNCTIONS AS A HELICASE WITHIN THE PRIMOSOME
ProductPutative primosomal protein N' PriA (Replication factor Y).
CommentsML0548, len: 651 aa. Putative priA, primosomal protein N', highly similar to PRIA_MYCTU|P71670|Rv1402 priA, putative primosomal protein N' from M. tuberculosis (655 aa), Fasta scores: E(): 0, (80.7% identity in 658 aa overlap); and CAD94298|Mb1437 from M. bovis (655 aa). Similar to many e.g. PRIA_ECOLI|P17888 priA, primosomal protein N' from Escherichia coli (732 aa), Fasta scores: E(): 1.2e-16, (28.1% identity in 445 aa overlap). COMPARED TO OTHER BACTERIAL PRIA, IT HAS A VERY DIVERGENT HELICASE DOMAIN. Belongs to the helicase family. PriA subfamily.
Functional categoryInformation pathways
Mutant
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS664261666216+
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
Protein sequence
>Mycobacterium leprae TN|ML0548|priA
MLSVPHLDREFDYLVSAEQSDDAQPGVRVRVRFHGRLVDGFVLERRNDTEHFGKLGWLDRVVSAQPVLTAEVRRLVDAVVARYAGTRPDVLRLAVPTRHARVERETLAIPVSPLPLIPGPVDPSGWEVYGRGGQFLTALAESRAARAVWQALPGEQWADRFAEAAAQAIRAGRAALAIVPDQRDLDVLWRAVTTRVDERSVVALSAGLGQAVRYQRWLKVLRGSARLVIGTRSAVFAPVNDLGLVMVWSDADDMLAEPRAPYPHAREVAMLRAYQARCAALIGGYTRTAEAHALVRSGWAHDVVAARSVVRARAPRVVALDDSGYAEESDPAARTARLPSIALRAARSALAAGAPVLVQVPRRGYVPSLACGRCRTLARCRHCTGPLSLLDRATPGTVCCWCGRADLTLRCARCGSEVVRAVVVGARRTAEELGRAFAGMPVITSVGDTIVPEVGARPALVVATPGAEPRAPGGYGAALLLDTWALLGRQDLRAAEEALWRWMTAAALVRARGDGGVVMVVAEASIPTVQSLMRWDPASHAEAELAARTEVGLPPSVHIAAVDGTTGAVNELLQEARLPDEADLLGPVDLPQGVRRPAGTPLGAPISRLLVRVPREQGWQLAASLRRGIGVLSVRQTHQLVRVQIDPLHIG
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