Gene ML0555 (ribD)
in Mycobacterium leprae TN
General annotation
| Type | CDS |
| Function | INVOLVED IN RIBOFLAVIN BIOSYNTHESIS (AT THE SECOND AND THIRD STEPS). CONVERTS 2,5-DIAMINO-6-(RIBOSYLAMINO)-4(3H)-PYRIMIDINONE 5'-PHOSPHATE INTO 5-AMINO-6-(RIBOSYLAMINO)-2,4(1H,3H)-PYRIMIDINEDIONE 5'-PHOSPHATE [CATALYTIC ACTIVITY 1: 2,5-DIAMINO-6-HYDROXY-4-(5-PHOSPHORIBOSYLAMINO)PYRIMIDINE + H(2)O = 5-AMINO-6-(5-PHOSPHORIBOSYLAMINO)URACIL + NH(3)] [CATALYTIC ACTIVITY 2: 5-AMINO-6-(5-PHOSPHORIBITYLAMINO)URACIL + NADP(+) = 5-AMINO-6-(5-PHOSPHORIBOSYLAMINO)URACIL + NADPH]. |
| Product | Probable bifunctional riboflavin-specific deaminase/reductase RibG: diaminohydroxyphosphoribosylaminopyrimidine deaminase (Riboflavin-specific deaminase) and 5-amino-6-(5-phosphoribosylamino) uracil reductase (HTP reductase) |
| Comments | ML0555, len: 339 aa. Probable ribG, bifunctional riboflavin biosynthesis protein, including diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5-amino-6-(5-phosphoribosylamino) uracil reductase (EC 3.5.4.26 and 1.1.1.193), highly similar to RIBD_MYCTU|P71677|Rv1409 ribG, bifunctional riboflavin biosynthesis protein from M. tuberculosis (339 aa), Fasta scores: E(): 0, (78.2% identity in 339 aa overlap); and CAD94305|Mb1444 from M. bovis (339 aa). Similar to many e.g. RIBD_BACSU|P17618 ribD, diaminohydroxyphosphoribosylaminopyrimidine deaminase / 5-amino-6-(5-phosphoribosylamino)-uracil reductase from Bacillus subtilis (361 aa), Fasta scores: E(): 7.9e-33, (39.2% identity in 357 aa overlap). Contains Pfam match to entry PF00383 dCMP_cyt_deam, Cytidine and deoxycytidylate deaminase zinc-binding region. Contains Pfam match to entry PF01872 RibD_C, RibD C-terminal domain. Contains PS00903 Cytidine and deoxycytidylate deaminases zinc-binding region signature. IN THE N-TERMINAL SECTION; BELONGS TO THE CYTIDINE AND DEOXYCYTIDYLATE DEAMINASES FAMILY. IN THE C-TERMINAL SECTION; BELONGS TO THE HTP REDUCTASE FAMILY. |
| Functional category | Intermediary metabolism and respiration |
| Mutant | Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 672088 | 673107 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium leprae TN|ML0555|ribG
VNVQTVDSLDAAMRLAVEQSYLVKGATYPNPPVGAVIVDPDGRVVGVGATEPAGGDHAEVVALRSAGVTAAGAIAVVTLEPCNHYGKTPPCVNALLEAKVDKVAYAIADPNASAGGGAARLTAAGVHVQSGVLADLVTAGPLREWLHKQRTGLPHVTWKYASSIDGRSAAADGSSRWLSSEASRADLHRRRATADAIVVGTGTVLADNPTLTARLANGALADRQPLRVVVGMREIPLEANVLNDDSPTMVIRTHDPMEVLKALADRTDVLLEGGPTLAGAFLRAGTINRILAYVAPILLGGPVTAVDDVGVPNIARALRWQFDGIDRAGSDLVISLVTR
Bibliography
No article yet recorded
