Gene ML0674 in Mycobacterium leprae TN
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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
Gene summary information
Gene namefolD
Gene length849 bp
IdentifierML0674
Location 810693 bp
Protein summary information
Molecular mass29803.2 Da
Isoelectric point6.85
Protein length283 amino acids
Structural information
PFAMPF00763, PF02882
Orthologs
M. tuberculosis H37RvRv3356c
M. lepromatosis Mx1-22AMLPM_0674
Cross-references
UniProtO32879
Gene OntologyFolic acid-containing compound biosynthetic process, Histidine biosynthetic process, Methenyltetrahydrofolate cyclohydrolase activity, Methionine biosynthetic process, Methylenetetrahydrofolate dehydrogenase (nadp+) activity, One-carbon metabolic process, Oxidation-reduction process, Purine nucleotide biosynthetic process, Binding
Enzyme Classification 3.5.4.9, 1.5.1.5
Precomputed results
BLASTReport
General annotation
TypeCDS
FunctionNECESSARY FOR THE BIOSYNTHESIS OF PURINES, THYMYDYLATE, METHIONINE, HISTIDINE, PANTOTHENATE, AND FORMYL TRNA-MET [CATALYTIC ACTIVITY: 5,10-METHYLENETETRAHYDROFOLATE + NADP(+) = 5,10-METHENYLTETRAHYDROFOLATE + NADPH] [CATALYTIC ACTIVITY: 5,10-METHENYLTETRAHYDROFOLATE + H(2)O = 10-FORMYLTETRAHYDROFOLATE].
ProductProbable bifunctional protein fold: methylenetetrahydrofolate dehydrogenase + methenyltetrahydrofolate cyclohydrolase FolD
CommentsML0674, len: 282 aa. Probable folD, bifunctional enzyme include methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) and methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), highly similar to O50385|AL123456|Rv3356c Probable folD, bifunctional enzyme from M. tuberculosis (281 aa), Fasta scores: E(): 0, (86.5% identity in 281 aa overlap); CAD95537|3391c from M. bovis (281 aa). Similar to many e.g. FOLD_ECOLI|P24186 folD, bifunctional methylenetetrahydrofolate dehydrogenase / methenyltetrahydrofolate cyclohydrolase from Escherichia coli (287 aa), Fasta scores: E(): 0, (40.8% identity in 277 aa overlap). Previously sequenced as O32879|Z98271 (282 aa), Fasta scores: E(): 0, (99.6% identity in 282 aa overlap). Contains Pfam match to entry PF00763 THF_DHG_CYH, Tetrahydrofolate dehydrogenase/cyclohydrolase. SIMILAR TO OTHER DEHYDROGENASE/CYCLOHYDROLASE ENZYMES OR DOMAINS.
Functional categoryIntermediary metabolism and respiration
Mutant
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS810693811541+
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
Protein sequence
>Mycobacterium leprae TN|ML0674|folD
VGAITLDGKATRDEILIDLKQRVAALTESGRTPGLGTILVGDDPGSHAYVRGKHADCAKVGITSIRRDLPVDITTAVLHDTIEELNANPDCTGYIVQLPLPKYLDENTALERVDPAKDADGLHPTNLGRLVLSTPAPLPCTARGILHLLRRYGVEIAGTHVVIIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRNLAALTKQADIIVAAVGVPHLLTADMVRPGAVVVDVGVSRVETRLVGDVHPDVWEVAGHVSPNPGGVGPLTRVFLLTNVVELAEGRQ
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