Gene ML0753 (rmlA2)
in Mycobacterium leprae TN
General annotation
| Type | CDS |
| Function | INVOLVED IN GDP-MANNOSE BIOSYNTHESIS AND BIOSYNTHESIS OF NUCLEOTIDE-ACTIVATED GLYCERO-MANNO-HEPTOSE (D-ALPHA-D PATHWAY): GENERATES GDP-MANNOSE AND PHOSPHATE FROM GTP AND ALPHA-D-MANNOSE 1-PHOSPHATE. MANB PRODUCT IS NEEDED FOR ALL MANNOSYL GLYCOLIPIDS AND POLYSACCHARIDES WHICH, LIKE RHAMNOSYL RESIDUES, ARE AN IMPORTANT PART OF THE MYCOBACTERIUM ENVELOPE [CATALYTIC ACTIVITY: ALPHA-D-MANNOSE 1-PHOSPHATE + GTP = GDP-MANNOSE + PHOSPHATE]. |
| Product | Probable D-alpha-D-mannose-1-phosphate guanylyltransferase ManB (D-ALPHA-D-HEPTOSE-1-PHOSPHATE GUANYLYLTRANSFERASE) |
| Comments | ML0753, len: 358 aa. Probable manB (alternate gene name: hddC), D-alpha-D-mannose-1-phosphate guanylyltransferase (EC 2.7.7.-) (see citations below), highly similar to Rv3264c|MTCY71.04c|P96869|Z92771 manB D-alpha-D-mannose-1-phosphate guanylyltransferase from Mycobacterium tuberculosis (359 aa), fasta scores: E(): 0, (86.9% identity in 359 aa); and CAD95384|Mb3292c from M. bovis (359 aa). Similar to several e.g. Q8FRK5 Putative mannose-1-phosphate guanyltransferase (372 aa), fasta scores: E(): 9.4e-93, (67.507% identity in 357 aa overlap); and Q9KZK6 Putative nucleotide phosphorylase from Streptomyces coelicolor (360 aa), fasta scores: E(): 6.7e-73, (56.704% identity in 358 aa overlap). Also similar to ML2503 from M. leprae. Contains Pfam match to entry PF00483 NTP_transferase, Nucleotidyl transferase. Contains 3 Pfam matches to entry PF00132 hexapep, Bacterial transferase hexapeptide (four repeats). BELONGS TO FAMILY 2 OF MANNOSE-6-PHOSPHATE ISOMERASES. Note that previously known as rmlA2. |
| Functional category | Cell wall and cell processes |
| Mutant | Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 895478 | 896554 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium leprae TN|ML0753|manB
LATLQVDAVVLVGGKGTRLRPLTLSAPKPMLPTAGLPFLTHLLSRIAAAGIEHVILSTSYRDAVFEAEFGDGSKLGLQIDYVIEESPLGTGGGIANVIDQLRHDTVMVFNGDVLSGVDLGQLLGFQRTNFADVTLHLVRVGDPRAFGCVSTEDGRVTAFLEKTQDPPTDQINAGCYVFERNVIDRIPRGREVSVEREVFPTLLSDADVKVCGYVDATYWRDMGTPEDFVRGSADLVRGIAPSPALGGHRGEHLVHDGAAVSPGAVLIGGTVVGRGAEIGPGVRLDGAVIFDGAKVEAGSVIERSILGFGVRIGPRALIRDGVIGDGADIGARCELLRGARVWPGVSIPDGGIRYSSDV
Bibliography
No article yet recorded
