Gene ML1003c
in Mycobacterium leprae TN
General annotation
| Type | CDS |
| Function | INVOLVED IN REGULATION OF NUCLEOTIDE EXCISION REPAIR AND SOS RESPONSE. REPRESSES A NUMBER OF GENES INVOLVED IN THE RESPONSE TO DNA DAMAGE (SOS RESPONSE), INCLUDING RECA AND LEXA. HAS BEEN SHOWN TO BIND TO THE 14 BP PALINDROMIC SEQUENCE 5'-CGAACNNNNGTTCG-3'. IN THE PRESENCE OF SINGLE-STRANDED DNA, RECA INTERACTS WITH LEXA CAUSING AN AUTOCATALYTIC CLEAVAGE WHICH DISRUPTS THE DNA-BINDING PART OF LEXA, LEADING TO DEREPRESSION OF THE SOS REGULON AND EVENTUALLY DNA REPAIR [CATALYTIC ACTIVITY: HYDROLYSIS OF ALA-|-GLY BOND IN REPRESSOR LEXA]. |
| Product | REPRESSOR LEXA |
| Comments | ML1003c, len: 235 aa. Probable lexA, repressor (EC 3.4.21.88). Identical to the previously sequenced Mycobacterium leprae LexA protein TR:Q49848 (EMBL:U00019) (235 aa), Fasta scores: E(): 0, 100.0% identity in 235 aa overlap. Also highly similar to many other LexA proteins known to repress genes in response to DNA damage, as part of the SOS response e.g. from Mycobacterium tuberculosis Rv2720 TR:Q50765 (EMBL:X91407) (217 aa), Fasta scores: E(): 0, 89.4% identity in 218 aa overlap and Escherichia coli SW:LEXA_ECOLI (P03033) (202 aa), Fasta scores: E(): 2.3e-10, 33.5% identity in 215 aa overlap. Note codon 19 represents an alternative possible translational start site. Contains Pfam match to entry PF00717 Peptidase_S24, Peptidase family S24. Contains Pfam match to entry PF01726 LexA_DNA_bind, LexA DNA binding domain. |
| Functional category | Regulatory proteins |
| Mutant | Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1175024 | 1175731 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium leprae TN|ML1003c|lexA
MSDSTDISGITVDGRLHSMDSGLTERQRTILNVIRASVTSRGYPPSIREIADAVGLTSTSSVAHQLRTLERKGYLRRDPNRPRAVNVRGVEETQAAGPAVLTEVAGSDVLPEPTFVPILGRIAAGSPIFAEGTVEDIFPLPRELVGEGTLFLLKVTGDSMVEAAICDGDWVVVRQQKVADNGDIVAAMIDGEATVKTFKRAGGQVWLIPHNPAFDPIPGNDATVLGKVVTVIRKI
Bibliography
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