Gene ML1226
in Mycobacterium leprae TN
General annotation
| Type | CDS |
| Function | QUINOLINATE BIOSYNTHESIS. CATALYZES THE OXIDATION OF L-ASPARTATE TO IMINOASPARTATE WHICH IS CONDENSED WITH DIHYDROXYACETONE PHOSPHATE TO QUINOLINATE UNDER THE ACTION OF QUINOLINATE SYNTHASE A [CATALYTIC ACTIVITY : L-ASPARTATE + H(2)O + O(2) = OXALOACETATE + NH(3) + H(2)O(2)] |
| Product | Probable L-aspartate oxidase NadB |
| Comments | ML1226, len: 286 aa. Probable nadB, L-aspartate oxidase (EC 1.4.3.16). Previously sequenced Mycobacterium leprae L-aspartate oxidase SW:NADB_MYCLE (Q49617) (424 aa), Fasta scores: E(): 0, 99.8% identity in 400 aa overlap. Note ML1226 differs in length from that previously sequenced, possibly due to a frameshift in the original sequence. Highly similar to several proteins involved in quinolinate biosynthesis e.g. Mycobacterium tuberculosis Rv1595 SW:NADB_MYCTU (O06595) (527 aa), Fasta scores: E(): 0, 76.7% identity in 529 aa overlap and Escherichia coli SW:NADB_ECOLI (P10902) (540 aa), Fasta scores: E(): 6.2e-31, 38.8% identity in 533 aa overlap. Contains Pfam match to entry PF00890 FAD_binding_2, FAD binding domain. |
| Functional category | Intermediary metabolism and respiration |
| Mutant | Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1447771 | 1449354 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium leprae TN|ML1226|nadB
MMAVPDWKHAADVVVIGTGVAGLAAALAAHRAGRNVVVLTKADQRRGETATHYAQGGIAVVLPGSDDSVDAHASDTLAAGAGMCNLDTVYSIVAEGYHAVTELVGYGARFDESIPGRWAVTCEGGHSRRRIVHAGGDATGAEVQRALDHAADVLDIRTSHLALRVLHDGTVVNGVSVLNPNGWGIVSAPSVILASGGLGHLYGATTNPEGSTGDGIALALWAGVAVSDLEFIQFHPTMLFAAGTGTGGRRPLVTEAIRGEGAILLDSKGNSVTSGVHPLGDLAPRDVVAAAIDARLKATGDSCVYLDARGIDGFASRFPTVTAACRTVGIDPAREPIPVVPGAHYSCGGIVTDAYGQTELAGLFAAGEVARTGMHGANRLASNSLLEGLVVGGRAGRAAAAHAAAAGRAYVSKLEPVTHHALKRRELQRVMSRDAAVMRNAAGLQRLSDTLAEAPIRHVTGRRDFEDVALTLTARAVAAAALARNESRGCHHCTEYPDTAPEHARSTVIRLADDQNLVRAEALATVG
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