Gene Rv0362
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Thought to be involved in Mg2+ transport. Responsible for the translocation of the substrate across the membrane. |
| Product | Possible Mg2+ transport transmembrane protein MgtE |
| Comments | Rv0362, (MTCY13E10.24), len: 460 aa. Possible mgtE, magnesium (Mg2+) transport transmembrane protein; C-terminal region is highly similar to MGTE|G780283 putative Mg2+ transporter from Providencia stuarti (314 aa), FASTA scores: E(): 0, (47.2% identity in 307 aa overlap) (N-terminus extends approx. 150 aa further upstream compared to P. stuarti ORF). Also similar in part to others e.g. AAK20879.1|AF334760_1|AF334760 putative Mg2+ transporter from Aeromonas hydrophila (455 aa); NP_231292.1|NC_002505 magnesium transporter from Vibrio cholerae (451 aa); NP_102305.1|NC_002678 Mg2+ transport protein from Mesorhizobium loti (454 aa); etc. Also similar to Rv1232c|MTV006.04c from Mycobacterium tuberculosis (435 aa). Extended hydrophobic segment spanning last 130 residues. Belongs to the MgtE family. |
| Functional category | Cell wall and cell processes |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, but essential for in vitro growth on cholesterol; by sequencing of Himar1-based transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 439871 | 441253 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv0362|mgtE
VSIRPAENSTLDIRHVIGIGTPKAVDLWLDVVTELPDRARELGSLSKAELGKLGPLLDGTNAVELFESIDDKLAAEALHAMDPSLAATFLEALDSDHAANILREFKEPKREALLTLLPLERAMVLRGLLSWPEDCAAAHMVPETLTVRPNMTVSQAVASVRERASGLRSDARTTAYVYVTDADSHLLGVIAFRALVLANPEQRVRELMGDDLIVVSPLTDKELAAQTIMGHNLMAVPVVDADNRLLGIIAEDEAIDIAEEEATEDAERQGGSAPLEVPYLRASPWLLWRKRVVWLLVLFAAEAYTGSVLRAFSDEMEAVIALAFFIPLLIGTGGNTGTQIATTLVRAMATGQVRFRDVPAVLAKELSTGVLVGLTMAAAAVVRAWTLGVGPQVTLTVALTVAAIVVWSSLVAAVLPPLLKKLRIDPAIVSGPMIATIVDGTGLLIYFLVAHLTLTELHGL
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
