Gene Rv0529 (ccsB)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Required during cytochrome biogenesis at the step of heme attachment. |
| Product | Possible cytochrome C-type biogenesis protein CcsA |
| Comments | Rv0529, (MTCY25D10.08), len: 324 aa. Possible ccsA, cytochrome C-type biogenesis protein, integral membrane protein, equivalent to NP_302558.1|NC_002677|B2168_C3_281 possible cytochrome C biogenesis protein from Mycobacterium leprae (327 aa), FASTA scores: opt: 1779, E(): 0, (82.9% identity in 327 aa overlap). Also highly similar to others e.g. CAC08382.1|AL392176 putative cytochrome biogenesis related protein from Streptomyces coelicolor (380 aa); CCSA_CHLRE|P48269 probable cytochrome c biogenesis protein from Chlamydomonas reinhardtii (353 aa), FASTA scores: opt: 449, E(): 1.3e-23, (34.4% identity in 247 aa overlap); etc. Belongs to the CCMF/CYCK/CCL1/NRFE/CCSA family. Note that previously known as ccsB. |
| Functional category | Intermediary metabolism and respiration |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 619891 | 620865 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv0529|ccsA
LNTLHVNVGLARYSDWAFTSAVVALVVALLLLAFEFAQVRGRGLAPLAVPAGSVATDSATPGIVADQRHRPFDERVGRGGLAVAYLGIGLLLACVVLRGLATQRVPWGNMYEFINLTCLSGLIAGAVVLRRARYRPLWVFLLVPVLILLTVSGRWLYANAAPVMPALQSYWLPIHVSVVSLGSGVFLVAGVASILFLVRTSRLGEPTGEGALAGMVRRLPDAQTLDGIAYRTTIFAFPVFGFGVIFGAIWAEEAWGRYWGWDPKETVSFVAWVVYAAYLHARSTAGWRDRKAAWINVAGFVAMVFNLFFVNLVTVGLHSYAGVG
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
