Gene Rv0557 (mtfB)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in lipomannan (LM) biosynthesis |
| Product | Mannosyltransferase MgtA |
| Comments | Rv0557, (MTCY25D10.36), len: 378 aa. MgtA (previously known as pimB), mannosyltransferase (see citation below), similar to other various transferases e.g. NP_243554.1|NC_002570 alpha-D-mannose-alpha(1-6)phosphatidyl myo-inositol monomannoside transferase from Bacillus halodurans (381 aa); NP_249533.1|NC_002516 probable glycosyl transferase from Pseudomonas aeruginosa (406 aa); NP_419573.1|NC_002696 glycosyl transferase, group 1 family protein, from Caulobacter crescentus (455 aa); etc. Also similar to Q55598 hypothetical 44.9 kDa protein from synechocystis SP (409 aa), FASTA scores: opt: 703, E(): 0, (33.9% identity in 378 aa overlap); GPI3_YEAST|P32363 n-acetylglucosaminyl-phosphatidylinositol biosynthetic protein (452 aa), FASTA scores: opt: 230, E(): 1.1e-07, (23.5% identity in 328 aa overlap). |
| Functional category | Lipid metabolism |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). M. tuberculosis Erdman Rv0557 mutant has reduced amounts of lipoarabinomannan (LAM) and lipomannan (LM) and kills human monocyte-derived macrophages at an increased rate (See Torrelles et al., 2009). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 648536 | 649672 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv0557|mgtA
VCGVRVAIVAESFLPQVNGVSNSVVKVLEHLRRTGHEALVIAPDTPPGEDRAERLHDGVRVHRVPSRMFPKVTTLPLGVPTFRMLRALRGFDPDVVHLASPALLGYGGLHAARRLGVPTVAVYQTDVPGFASSYGIPMTARAAWAWFRHLHRLADRTLAPSTATMESLIAQGIPRVHRWARGVDVQRFAPSARNEVLRRRWSPDGKPIVGFVGRLAPEKHVDRLTGLAASGAVRLVIVGDGIDRARLQSAMPTAVFTGARYGKELAEAYASMDVFVHSGEHETFCQVVQEALASGLPVIAPDAGGPRDLITPHRTGLLLPVGEFEHRLPDAVAHLVHERQRYALAARRSVLGRSWPVVCDELLGHYEAVRGRRTTQAA
Bibliography
- Schaeffer ML, Khoo KH, Besra GS, Chatterjee D, Brennan PJ, Belisle JT and Inamine JM [1999]. The pimB gene of Mycobacterium tuberculosis encodes a mannosyltransferase involved in lipoarabinomannan biosynthesis. Product Biochemistry Function
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Tatituri RV, Illarionov PA, Dover LG, Nigou J, Gilleron M, Hitchen P, Krumbach K, Morris HR, Spencer N, Dell A, Eggeling L and Besra GS [2007]. Inactivation of Corynebacterium glutamicum NCgl0452 and the role of MgtA in the biosynthesis of a novel mannosylated glycolipid involved in lipomannan biosynthesis. Function Product
- Mishra AK et al. [2009]. Characterization of the Corynebacterium glutamicum deltapimB' deltamgtA double deletion mutant and the role of Mycobacterium tuberculosis orthologues Rv2188c and Rv0557 in glycolipid biosynthesis. Function Product
- Torrelles JB, DesJardin LE, MacNeil J, Kaufman TM, Kutzbach B, Knaup R, McCarthy TR, Gurcha SS, Besra GS, Clegg S and Schlesinger LS [2009]. Inactivation of Mycobacterium tuberculosis mannosyltransferase pimB reduces the cell wall lipoarabinomannan and lipomannan content and increases the rate of bacterial-induced human macrophage cell death. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
